LOCUS       DAMKMI010000001        98679 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMKMI010000001 DAMKMI010000000
VERSION     DAMKMI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 98679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.001"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            109..300
                     /locus_tag="PC429_000001"
     CDS             109..300
                     /locus_tag="PC429_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF3845532.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="PC429_000002"
     CDS             455..1864
                     /locus_tag="PC429_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3845533.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="PC429_000003"
     CDS             1995..2684
                     /locus_tag="PC429_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF3845534.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="PC429_000004"
     CDS             2687..3622
                     /locus_tag="PC429_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF3845535.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMNGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="PC429_000005"
     CDS             3619..4512
                     /locus_tag="PC429_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF3845536.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="PC429_000006"
     CDS             4590..5336
                     /locus_tag="PC429_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF3845537.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="PC429_000007"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="PC429_000007"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF3845538.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFIFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="PC429_000008"
     CDS             6193..6897
                     /locus_tag="PC429_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF3845539.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="PC429_000009"
     CDS             complement(7085..7513)
                     /locus_tag="PC429_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF3845540.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="PC429_000010"
     CDS             complement(7550..8980)
                     /locus_tag="PC429_000010"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF3845541.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="PC429_000011"
     CDS             9112..9696
                     /locus_tag="PC429_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF3845542.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="PC429_000012"
     CDS             9712..10233
                     /locus_tag="PC429_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF3845543.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="PC429_000013"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="PC429_000013"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF3845544.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="PC429_000014"
     CDS             12596..13366
                     /locus_tag="PC429_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF3845545.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="PC429_000015"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="PC429_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF3845546.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="PC429_000016"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="PC429_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF3845547.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="PC429_000017"
     CDS             14727..15812
                     /locus_tag="PC429_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF3845548.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="PC429_000018"
     CDS             15832..16701
                     /locus_tag="PC429_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF3845549.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="PC429_000019"
     CDS             16694..17560
                     /locus_tag="PC429_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3845550.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="PC429_000020"
     CDS             17601..18314
                     /locus_tag="PC429_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3845551.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18315..19484
                     /locus_tag="PC429_000021"
     CDS             18315..19484
                     /locus_tag="PC429_000021"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF3845552.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="PC429_000022"
     CDS             19488..20324
                     /locus_tag="PC429_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF3845553.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="PC429_000023"
     CDS             20325..21320
                     /locus_tag="PC429_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3845554.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="PC429_000024"
     CDS             21338..23026
                     /locus_tag="PC429_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF3845555.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGTSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="PC429_000025"
     CDS             23038..24105
                     /locus_tag="PC429_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3845556.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDVMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
     gene            24542..25537
                     /locus_tag="PC429_000026"
     CDS             24542..25537
                     /locus_tag="PC429_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3845557.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            25555..27243
                     /locus_tag="PC429_000027"
     CDS             25555..27243
                     /locus_tag="PC429_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF3845558.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            27255..28322
                     /locus_tag="PC429_000028"
     CDS             27255..28322
                     /locus_tag="PC429_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3845559.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            28514..29044
                     /gene="rnmV"
                     /locus_tag="PC429_000029"
     CDS             28514..29044
                     /gene="rnmV"
                     /locus_tag="PC429_000029"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF3845560.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            29215..30084
                     /gene="rsmA"
                     /locus_tag="PC429_000030"
     CDS             29215..30084
                     /gene="rsmA"
                     /locus_tag="PC429_000030"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF3845561.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            30150..31415
                     /locus_tag="PC429_000031"
     CDS             30150..31415
                     /locus_tag="PC429_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845562.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIDEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            31566..32681
                     /locus_tag="PC429_000032"
     CDS             31566..32681
                     /locus_tag="PC429_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF3845563.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            32867..33763
                     /locus_tag="PC429_000033"
     CDS             32867..33763
                     /locus_tag="PC429_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF3845564.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            33833..34630
                     /locus_tag="PC429_000034"
     CDS             33833..34630
                     /locus_tag="PC429_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845565.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(34685..36037)
                     /locus_tag="PC429_000035"
     CDS             complement(34685..36037)
                     /locus_tag="PC429_000035"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF3845566.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            36256..37134
                     /gene="purR"
                     /locus_tag="PC429_000036"
     CDS             36256..37134
                     /gene="purR"
                     /locus_tag="PC429_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF3845567.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            37290..37568
                     /gene="spoVG"
                     /locus_tag="PC429_000037"
     CDS             37290..37568
                     /gene="spoVG"
                     /locus_tag="PC429_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF3845568.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            37781..39160
                     /gene="glmU"
                     /locus_tag="PC429_000038"
     CDS             37781..39160
                     /gene="glmU"
                     /locus_tag="PC429_000038"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF3845569.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            39255..40205
                     /locus_tag="PC429_000039"
     CDS             39255..40205
                     /locus_tag="PC429_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF3845570.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      41049..41134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            41296..41817
                     /locus_tag="PC429_000040"
     CDS             41296..41817
                     /locus_tag="PC429_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3845571.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            41969..43645
                     /locus_tag="PC429_000041"
     CDS             41969..43645
                     /locus_tag="PC429_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF3845572.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            43657..44865
                     /locus_tag="PC429_000042"
     CDS             43657..44865
                     /locus_tag="PC429_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF3845573.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            44880..46592
                     /locus_tag="PC429_000043"
     CDS             44880..46592
                     /locus_tag="PC429_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF3845574.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            46585..47493
                     /gene="pilO"
                     /locus_tag="PC429_000044"
     CDS             46585..47493
                     /gene="pilO"
                     /locus_tag="PC429_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF3845575.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            47520..48086
                     /locus_tag="PC429_000045"
     CDS             47520..48086
                     /locus_tag="PC429_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3845576.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            48123..48650
                     /locus_tag="PC429_000046"
     CDS             48123..48650
                     /locus_tag="PC429_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3845577.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            48671..50209
                     /locus_tag="PC429_000047"
     CDS             48671..50209
                     /locus_tag="PC429_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845578.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRNSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            50309..51391
                     /locus_tag="PC429_000048"
     CDS             50309..51391
                     /locus_tag="PC429_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF3845579.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            51372..52154
                     /locus_tag="PC429_000049"
     CDS             51372..52154
                     /locus_tag="PC429_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF3845580.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            52256..52906
                     /locus_tag="PC429_000050"
     CDS             52256..52906
                     /locus_tag="PC429_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF3845581.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            52920..53480
                     /locus_tag="PC429_000051"
     CDS             52920..53480
                     /locus_tag="PC429_000051"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF3845582.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            53497..56883
                     /gene="mfd"
                     /locus_tag="PC429_000052"
     CDS             53497..56883
                     /gene="mfd"
                     /locus_tag="PC429_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF3845583.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            56914..57909
                     /locus_tag="PC429_000053"
     CDS             56914..57909
                     /locus_tag="PC429_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3845584.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            58033..58572
                     /gene="spoVT"
                     /locus_tag="PC429_000054"
     CDS             58033..58572
                     /gene="spoVT"
                     /locus_tag="PC429_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF3845585.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            58692..60305
                     /locus_tag="PC429_000055"
     CDS             58692..60305
                     /locus_tag="PC429_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF3845586.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            60412..61860
                     /gene="mazG"
                     /locus_tag="PC429_000056"
     CDS             60412..61860
                     /gene="mazG"
                     /locus_tag="PC429_000056"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF3845587.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            61949..62227
                     /locus_tag="PC429_000057"
     CDS             61949..62227
                     /locus_tag="PC429_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF3845588.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            62299..62541
                     /locus_tag="PC429_000058"
     CDS             62299..62541
                     /locus_tag="PC429_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF3845589.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYKILE"
     gene            62603..62857
                     /locus_tag="PC429_000059"
     CDS             62603..62857
                     /locus_tag="PC429_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF3845590.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            62858..63391
                     /locus_tag="PC429_000060"
     CDS             62858..63391
                     /locus_tag="PC429_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845591.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            63397..63681
                     /locus_tag="PC429_000061"
     CDS             63397..63681
                     /locus_tag="PC429_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF3845592.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            63794..64714
                     /locus_tag="PC429_000062"
     CDS             63794..64714
                     /locus_tag="PC429_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF3845593.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            64804..67176
                     /gene="spoIIE"
                     /locus_tag="PC429_000063"
     CDS             64804..67176
                     /gene="spoIIE"
                     /locus_tag="PC429_000063"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF3845594.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            67527..69419
                     /gene="pepF"
                     /locus_tag="PC429_000064"
     CDS             67527..69419
                     /gene="pepF"
                     /locus_tag="PC429_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF3845595.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            69533..70498
                     /gene="galU"
                     /locus_tag="PC429_000065"
     CDS             69533..70498
                     /gene="galU"
                     /locus_tag="PC429_000065"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF3845596.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            70998..72602
                     /gene="rho"
                     /locus_tag="PC429_000066"
     CDS             70998..72602
                     /gene="rho"
                     /locus_tag="PC429_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF3845597.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            72755..72955
                     /gene="rpmE"
                     /locus_tag="PC429_000067"
     CDS             72755..72955
                     /gene="rpmE"
                     /locus_tag="PC429_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF3845598.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            73100..73993
                     /locus_tag="PC429_000068"
     CDS             73100..73993
                     /locus_tag="PC429_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF3845599.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            74011..74859
                     /gene="prmC"
                     /locus_tag="PC429_000069"
     CDS             74011..74859
                     /gene="prmC"
                     /locus_tag="PC429_000069"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF3845600.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            74903..75967
                     /gene="prfA"
                     /locus_tag="PC429_000070"
     CDS             74903..75967
                     /gene="prfA"
                     /locus_tag="PC429_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF3845601.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            76096..76776
                     /locus_tag="PC429_000071"
     CDS             76096..76776
                     /locus_tag="PC429_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF3845602.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            76805..77845
                     /locus_tag="PC429_000072"
     CDS             76805..77845
                     /locus_tag="PC429_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF3845603.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            77862..78311
                     /locus_tag="PC429_000073"
     CDS             77862..78311
                     /locus_tag="PC429_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF3845604.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            78328..78780
                     /gene="rpiB"
                     /locus_tag="PC429_000074"
     CDS             78328..78780
                     /gene="rpiB"
                     /locus_tag="PC429_000074"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF3845605.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            78816..79445
                     /gene="upp"
                     /locus_tag="PC429_000075"
     CDS             78816..79445
                     /gene="upp"
                     /locus_tag="PC429_000075"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF3845606.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            79746..80561
                     /locus_tag="PC429_000076"
     CDS             79746..80561
                     /locus_tag="PC429_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF3845607.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            80787..81224
                     /locus_tag="PC429_000077"
     CDS             80787..81224
                     /locus_tag="PC429_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF3845608.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            81409..81636
                     /locus_tag="PC429_000078"
     CDS             81409..81636
                     /locus_tag="PC429_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF3845609.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            81641..82027
                     /locus_tag="PC429_000079"
     CDS             81641..82027
                     /locus_tag="PC429_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF3845610.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            82030..82734
                     /gene="atpB"
                     /locus_tag="PC429_000080"
     CDS             82030..82734
                     /gene="atpB"
                     /locus_tag="PC429_000080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF3845611.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            82803..83063
                     /gene="atpE"
                     /locus_tag="PC429_000081"
     CDS             82803..83063
                     /gene="atpE"
                     /locus_tag="PC429_000081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF3845612.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            83186..83683
                     /gene="atpF"
                     /locus_tag="PC429_000082"
     CDS             83186..83683
                     /gene="atpF"
                     /locus_tag="PC429_000082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF3845613.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLVEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            83680..84225
                     /locus_tag="PC429_000083"
     CDS             83680..84225
                     /locus_tag="PC429_000083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF3845614.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            84242..85744
                     /locus_tag="PC429_000084"
     CDS             84242..85744
                     /locus_tag="PC429_000084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF3845615.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            85772..86650
                     /gene="atpG"
                     /locus_tag="PC429_000085"
     CDS             85772..86650
                     /gene="atpG"
                     /locus_tag="PC429_000085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF3845616.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            86663..88057
                     /gene="atpD"
                     /locus_tag="PC429_000086"
     CDS             86663..88057
                     /gene="atpD"
                     /locus_tag="PC429_000086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF3845617.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            88060..88320
                     /gene="atpC"
                     /locus_tag="PC429_000087"
     CDS             88060..88320
                     /gene="atpC"
                     /locus_tag="PC429_000087"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF3845618.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            88782..89162
                     /locus_tag="PC429_000088"
     CDS             88782..89162
                     /locus_tag="PC429_000088"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF3845619.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            89177..89638
                     /locus_tag="PC429_000089"
     CDS             89177..89638
                     /locus_tag="PC429_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF3845620.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            89659..90249
                     /locus_tag="PC429_000090"
     CDS             89659..90249
                     /locus_tag="PC429_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845621.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            90264..91421
                     /gene="alr"
                     /locus_tag="PC429_000091"
     CDS             90264..91421
                     /gene="alr"
                     /locus_tag="PC429_000091"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF3845622.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            91454..91741
                     /locus_tag="PC429_000092"
     CDS             91454..91741
                     /locus_tag="PC429_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF3845623.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            91710..92093
                     /locus_tag="PC429_000093"
     CDS             91710..92093
                     /locus_tag="PC429_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF3845624.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            92264..93091
                     /locus_tag="PC429_000094"
     CDS             92264..93091
                     /locus_tag="PC429_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF3845625.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            93084..94025
                     /locus_tag="PC429_000095"
     CDS             93084..94025
                     /locus_tag="PC429_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF3845626.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            94448..95542
                     /locus_tag="PC429_000096"
     CDS             94448..95542
                     /locus_tag="PC429_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845627.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            95768..96184
                     /locus_tag="PC429_000097"
     CDS             95768..96184
                     /locus_tag="PC429_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845628.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(96366..96935)
                     /locus_tag="PC429_000098"
     CDS             complement(96366..96935)
                     /locus_tag="PC429_000098"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF3845629.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            97089..98246
                     /locus_tag="PC429_000099"
     CDS             97089..98246
                     /locus_tag="PC429_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF3845630.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
BASE COUNT        39525 a        10642 c        18100 g        30412 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atatcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaataa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagtatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag atatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagttaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgaatgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggcattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaat attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttattt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaaa acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagttatgc tgatatctga aaagccagca ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacggcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agatgcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaactaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atgggttaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactaa ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacta
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcgaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgtgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggtacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagtttat gaggcactct ttttgtatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagatag tttgttttta tttatttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagttgtt tattatatta ttaaagagaa gtaaacgaaa
    13681 agtagattaa ggaggttgaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaacac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttatttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcat ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcacttac aatgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctagca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt atagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttgaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gacattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcatta actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggggatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgagga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatga ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagaccat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtaa ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggagcttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatgac aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 agattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccga
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagcataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaagaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttgtaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaagattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctctggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaaatctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggttcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaag agtacactac
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga agttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa cataaagttt aaagattttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatatg atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc cataaatatt ccattctata aagtattttt
    22741 taatataaca gttccaatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat acacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaacatcag ctgctatggc
    22921 agtattagtg cttttaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggtaaaaatg
    23041 aaatttaaga agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggtgaagaa
    23641 aaagcatggc agtatatgga taaattgcat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa agttaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgtaatga acgaatactc taagaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaagaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttagaga ttaatttaga aatagatata ctacagttta
    24301 aaatagtata ttttaatttc attattaaag tatagatatt atatataaag taaataaatt
    24361 attgagcgat tattaataag atatgtataa attttaattt gggaacttgt gttatatatc
    24421 atgaaaaaag agaaaaaata aaaggtaaat tgtttcttga taacttgaaa ttggaagtga
    24481 gaatatttaa ctagagaaga attatttaag tttaagtcag ctttaggagg aggaattata
    24541 aatgtctttt ttacaagtaa aaaatgtagg taaaagttat ggacaagtta aggtgctaaa
    24601 agacatatct attgatattg aaaaagggga gtttatttgc ttattaggtc caagtggttg
    24661 tggtaaaagt acattgctta gaataattgc aggacttgag gataaacatg atggaaaaat
    24721 aataataaat gataaggata tgactaactc accacctgaa agtagaaact ttggtattgt
    24781 tttccaatca tatgcactat ttccaaatat gaatgtatat aaaaatatag cctttggatt
    24841 agaaaataaa aatataagta aatctgatat agataagaag gttagagata ttttagaagt
    24901 tgtagaatta agtggatatg ataaaaaata tccatcacaa ttatcaggtg gacaacaaca
    24961 aagggttgca ctagctcgtg cgattgcttt agagccagac tttttgcttc ttgatgaacc
    25021 tttatctgca ttagatgcaa aagtaagact taaacttagg gaacagatta aaagtttaca
    25081 tagaaaacta ggcatcacaa ctataatggt aacacatgac caagaggaag ctctttgttt
    25141 agctgataaa atggttgtta tgaataatgg agaggtaata cagattggaa ctcctaaaga
    25201 agtctataaa agtccaaaaa caccttttgt agctgatttt ataggaacaa taaattttat
    25261 agatgatgat gtgaatatga tagcaattag accagaagat ataaaggtag aatttaatag
    25321 agactttaaa gaaattgatg taaaagtagg agagattttg gatatagaat ttagaggatt
    25381 caactataga attacagttg aatattgttc taagcaaata aagttggacg tagtttcaaa
    25441 ggtagtggaa caaatgaaac tatgtgtagg ttctaaaata aacttcaaga tacctaaaga
    25501 aagaaccata caatataaat cagagggatg tgcttaatag ctagaggtgg ataaatgata
    25561 acaataaaaa ataaaagaag aataagtgat agtaagatac aaaaatgttt tttagttttt
    25621 ttagtgattg cattagtagt atttatatta tttccattaa tatcactatt aaaaaatgca
    25681 tttatggata caagtggaag ttttgtagga atttcaaact ttagtaaata tattgaaaat
    25741 cccactctta tagcatcatt taaaaactca atttttgtat ctacaatttc atccttaata
    25801 tcattatgtt tagcgtttat atattcatat gcaataacaa gaacgaatat aaggtttaaa
    25861 aactttttta aaactatggg catatttccg cttttcgcac cagctatgct atatggtatt
    25921 tcacttatat acctttttgg aaacaaggga atctttacaa ttataggcat agaagtacca
    25981 ctatatggtc cattaggaat aattatttgt caaatactgt ttacatttcc acaggccttt
    26041 ttaatattat ctgtagcact ttcgatggct gattatagac tttatgaagc agcagatagc
    26101 ttaggggcta gtgaatttaa gaagttcttc aaaataacaa ttcctggtat aaagtattca
    26161 ttagttagtt cgttctttgt atcttttata cttgcattta cagattttgg agcagcaaag
    26221 atagttggag gaaactacaa tgtattggct actgacatat ataaacaagt ggtaggtcaa
    26281 tttaacatac caatgggagc tactgtctct atggtaatgt taattcctgt actaatagca
    26341 tttacagtag ataagatagc aagtaaaaag caagggatga ctataactgc aaagtctatg
    26401 ccatataaga ttaaggaaaa taagtttaga gatagagtat ttttaatagc atgtagtttt
    26461 ataactttat taatctttat tttgatattt gtttcagtta taggctcagt tataaaatta
    26521 tggccatatg atttaagctt tacaatggaa cactatacgt ttaaaggtat atcaggaaat
    26581 ggaatcaaaa catatttaag cagtttaaaa atatcaattt taacagcaat atttggaact
    26641 atatttgtat tttttagtgc ttatatgata gaaaaagtgg ataagtttcc tagactgaga
    26701 caaatatctt attttttatc aatgatacca atggcattac ctggtttggc tttaggtata
    26761 tcgtatataa cttttttcaa ttcagcaagt aatccattaa attttttata tggaagtaca
    26821 acaataatgg ttatagccaa tatagttcat ttttattcag tttgctttat aacttcaaat
    26881 tcatctttaa aaatgttgga tagagagtat gaattagttg caaaatctat aaatattcca
    26941 ttttataagg tgttttttta tgtaacactt ccaatgtcca taacatcaat acttgaagtg
    27001 gctgtatatt acttcgtaaa ttcaatggtt actgtttcag cattaatatt tttatatata
    27061 cctcaaactc aaactgcatc aatatcgata ctaaagttag aagagatagg gtatataggg
    27121 ccatcagctg ctatggcagt attagtgttt ttaacaaata taatagtaaa acttttgtat
    27181 gaattgacaa caaagaaatt aaaaactaag acacaaaaat ggcaacaaaa agaagttgca
    27241 tagaggaggt aaaaatgaaa tttaggaaat tagtctcttt aggaatatta tcaacacttt
    27301 tattgagtgt aataactgga tgtaataatg cttcatcagg ttcaaattta aagtctaata
    27361 ataagataaa aggagagtta acagtttata cagcaattga agaagatagt atagatacat
    27421 atttaactac atttaaagag aaatatccag atattaaatt gaatgtactt agattatcag
    27481 taggagatat aacagcaaga ttattagcag aaaaagataa tcctaaagca gatgtgttat
    27541 ggggggttgc agctacaagt ttgataattt tagatgacaa agaaatgcta gagccatacg
    27601 ctccgaaagg ctttgataag gtaaatcctg tatttaaaga tgataagaaa gttccttctt
    27661 gggttggaat agatgcttgg atgactggta taacagtaaa tactaaagaa ttaagtgata
    27721 agaacatacc agttcctagt tcttatgaag atttaataaa gccagaatat aaaggtctta
    27781 tatctatgcc taatccagct tcttctggaa caggatattt aacagtatca gaacttatac
    27841 aaattatggg tgaagaaaaa gcatggcagt atatggataa attgcataaa aatattggtg
    27901 tatacactca gtctggttca gcgccagcta tattagcagc ttcaggagaa tatcccattg
    27961 gtatatcttt tggaaataga ggtataaagt taaaaggtga aggttatcca gtagaagtag
    28021 tatttccaaa agaaggttct ggatgggaga tagaatcaaa tgcacttgtg aaaaaagata
    28081 atataaaaga agcatcaaaa gtatttttag attgggctat aagtaaagat gcaatgaatg
    28141 agtattctaa gaattttgca gttactacaa ttgacacggg aaatccggtg ccagaaggat
    28201 ttccaaagaa tccattagaa cagatgatag ataatgattt aaaatcagca gcaagaaata
    28261 gagaggctat actaaataaa tggatagaaa aatatgatgg aaagacagaa aaagagagct
    28321 aagccttgca ataaaattta gtacaaaaag cgcctagaat ataagtttta tatctaggct
    28381 cttttgatat atttatttaa aatgcatatt tctatataaa aataaatagt ttagtacctt
    28441 gattaaatta aataaaaacc ttaaaataaa gagatgtaaa gaaaaactaa aaacgaataa
    28501 aaggtgaatt tatatgataa aagaaataat agtagtagag ggtagagatg acgttacagc
    28561 agttaagaga gcaatagatg cagaacttat aactacaggt ggatttggat ttccaaaagg
    28621 tgtaatggag cgtataaaag ctgctaatga aagacgtggt gttataatat ttacagaccc
    28681 agattttgca ggtgaaaaaa taagaaaaaa aatagcatca gaagttccag gatgtaaaca
    28741 tgcatttcta ccgagagagg aagctaaaaa ggatggagat ataggtattg agaatgctac
    28801 tcctcaaagt ataatagcag ctctgaataa agttagaact gaaaatacag aaaaaagaga
    28861 tgaatttaag caagttgact taattagaaa tggtcttata ggtaatgaag aagcatcact
    28921 tagaagagat gcacttggaa aaatacttgg aattggatat ggaaatgcaa aacaattttt
    28981 aaatagattg aataattatg gagttgaaag agaagaattt atagatgcat taaaacaaat
    29041 ctaaacgtaa tgtttaatgt atgaaaaata tgtatataaa ataaattgtg gaattacggt
    29101 taatttaaga tgaattattt ataatataca ccttatttta aatcaatgta aatacatata
    29161 aataagtata attaataaaa ttaaatatca aaataagtta aaaaggagta aataatggat
    29221 agactatctt cacataatgc aacaaaggaa gtagtacaaa aatataattt taaattttca
    29281 aagtctttag gtcaaaattt cttgatagat agtaatataa ttgataaaat cttatctgga
    29341 gctagaatca caagaggaga taatatcata gaagtaggac ctggtatagg aactcttact
    29401 cgtgagatgg gtaaaatagc agaaaaagta gtggctatag aaatcgatag aaatttaatt
    29461 cctatattga aagatacctt gtcagattta gataatacag aggttgtaaa tcaagatata
    29521 ctaaaagtag acatacagga attagtaaaa gataaattaa atggtgggcc agttaagctt
    29581 gtagcaaatc ttccatatta tataactact ccaatagtta tgaaattttt agaggaagac
    29641 atacctgtaa cagacatagt agtcatggtt caaaaagaag ttgcagatag aatgaatgct
    29701 attccgggta ctaaagatta tggggcatta tctatagctg ttcaatacta ttgtgataca
    29761 gagattgtgg caaaagctcc aagacatatg tttataccac aaccaaatgt agattccaca
    29821 gttataggtc tacatgtaag agataaaaga aaatatgatg ttcataatga agacatattt
    29881 tttaaaacag taaaagcatc ctttggacaa agaagaaaaa ctcttcttaa ttcgttggga
    29941 ggtcttggtt ttcttaataa agatgaaata agagaagtat taaaagaagc taatatagac
    30001 gaaaaaagaa ggggtgaaac tcttagtata gaagagtttt cagttctttc aaatataata
    30061 aatacaaaag tatcttctaa atagaaggta ctttttttaa ttttgttaac atatattgtt
    30121 tagagaaact gtgttaaggg gggattgcag tgggttctaa aagaaagttt aaaagagact
    30181 atataatact agaagctaaa gacataaact ttagatacaa agaacgagta ctgccaaaag
    30241 cttttgcaaa agttgaagta aatgatgaaa aagcaattat tgcattatat gtagaaaatc
    30301 taaaatatgt taaagatggg tataaagcag tagcaataaa agatgaccat ggattaatag
    30361 acttaggaaa tataatttta aatgaacaag gaagaggtga atttgtttta gatttagaag
    30421 attatgatga tgagataaaa ggtattgcat tattgtatga aagaaatata cctttaattg
    30481 gatttaaagg aaataagctt gaaaattatg aggacatatt atttcaagag gtagaggaag
    30541 aagaatatga agaatatgaa gagttggaag aagaagacga agagatagag gataatgagg
    30601 aaagtgatag agacgaaggt agtgaagaag aatatattat agaagaatat gatgaagatg
    30661 agtatgaaga ctattgggaa gatgatgaag atattgacga aggagagtta aaagcaagaa
    30721 cggaagtaga agattctggt gatgaggaaa tagaagagtt caataattat aaggaaatag
    30781 aaaattataa tagggttaaa gtacaggaag aatatataga catagaatat gatgaggaag
    30841 aagaggaaga agaagaagtt tttgaagtaa atagagaaga agataggttt gtttcctcaa
    30901 aacctactaa atcaaagaaa gttcaaaaag atcagaataa tattgaaaaa ataaaaagag
    30961 ctaatggagt aagtcaagga gagagtggaa ataaacaaac cagtacatta cttatgccaa
    31021 gacaaataaa aaaaggtttg aaattattta aagaagtaaa accttttgta acagattata
    31081 tagataagac aagatggtgg aaaatagaga taaatcctac aacattgtgt ggatatacaa
    31141 tgccttattt aggatattta aacgtgttga attacaccat gtatagtgat gcagttttac
    31201 attcatataa atacagacac tatttatttg gagtccagta tgatgaatat aataagagaa
    31261 aacattatat ttatgctatc ccaggaaata gaaatgaaca gccagacaat ggaagcacag
    31321 gatttttaag atatcaagct tgtgataata gaacaaatag tttaggatat tggctatgtt
    31381 ttatagattc tagacaaaga aatatagtta ggtaaaaata ataaaaatga aaaaatttag
    31441 actcaattct tattttactg aaaaaattaa tgaaatgtag tacaaaactt taaaaactat
    31501 tgataatatt taaatattac actacaatat atcttgaaat acctattata ttatggagga
    31561 aaattatgat aaacgagcaa agaattttaa atgagtttct tgaattagta cagatagata
    31621 gtttatcttt aaaagagggg aatgttgcta aagtattagt taaaaaatta gaagaaatag
    31681 gttgttcagt agtaatagac aatgctggtg aaaaagcaaa tggagaaact ggtaatataa
    31741 tagctacatt aaaaggaaat aaagaaggaa agaaaatatt atttagttca catatggata
    31801 cagtaactcc aggtataggg gtaaaaccta tagtggatga agcaaatgga ataataaaaa
    31861 gtgatggtac tacaatatta ggttctgatg ataaagcagg tatagcagct atattagaag
    31921 gcttaagata tataaatgaa aataatatag aacatactga tatacaagtt gtattctcta
    31981 tatgtgagga atgtggatta gttggagcta aaaatttaga ctatggaaaa atagattctg
    32041 aatatgcatt tatattagat agtggtggtt ctccaggtga aataatagta aaagctcctg
    32101 cacaagatgt aataaatgtt aagatattag gaaagactgc tcatgcaggt cttgaacctg
    32161 aagctggaat aagtgctata atggtagctg ccagagcaat agaaaatatg aatttactta
    32221 gaatagacga agaaacaact gctaacatag gaattataaa cggtggaaca gctacaaata
    32281 tagtaacagg agaagttaat ataattgcag aagccagaag tctaaaagaa aataaattag
    32341 atgttcaaac taaacacatg gtagagactt ttgaaaaagc tgcaaaagat tttggagctc
    32401 aaattgagat agatgtcaat agagcatata caccaataga tgttagtgag gattctgaaa
    32461 taataaaact tgcaaagaag gctttttcaa acttaggtat agaaggtcat actgaatcta
    32521 caggaggagg tagtgataca aatatattaa gtaagaatgg tattgaagct ataactttag
    32581 gaattggtat gaaaaatgct catacactaa gtgaacatat agctataaaa gacttatatg
    32641 attctgctaa aatggtagtg gagataataa aggaagcata aatttaaata atagtgtata
    32701 atatatatat cgcctagctt aagctaggtg atttttcatt ttatagaaga ttgataatct
    32761 ctattgaaag taaagagtat acatttgcat ataagagagt gtttaattta tgttcaaatg
    32821 taggttctta ataatattta gattgtttca taagggagga ataaatatgg aaacaaggta
    32881 tgaagaagca aataaaataa caatacaatc aatactttgg aatgtagtat taacaataat
    32941 taaggttata gctggtgtta ttggtaattc tagcgctatg attgcggatg gattacattc
    33001 agcatcagat attataagtt ctataggagt actaataggc aattatgtat cttctagacc
    33061 tggagataga gagcataatt atggtcatga gaaagcagag acattagtat catttgtgtt
    33121 atcaatattg cttattttcg tttctataac aataggtata gaggcaatta aatctttatt
    33181 taacttagat gcattaagtg ttccatcaat attaccatta gtagtatcag ttatttcaat
    33241 cctgataaaa gagtatcaat atagaataac aataaaagtt gctaaaaaga taaattcacc
    33301 agccctaaaa gctgatgctt ggcatcatag gtcagatgct ttatcttcag tagctgcttt
    33361 tataggtata ggtggttcta ttttagggtt taaaccatta gacccaattg cgtcagttgt
    33421 agtggctata tttgtagcta aagtaggaat tagtatactt ataagttctg ttaatgaatt
    33481 gatggatgtt tcagttgatg aagaagagat aaaagaatta aaatttattg tagcagatac
    33541 agaaggtgtt aaaaatcttg gtgatataaa gactagaaaa catggagcta tggcatatgt
    33601 agatttgact atatgtgtgg atgaaaattt gacagttaag caaggtcatg atattgctac
    33661 caagcttgaa aaacatataa taaaacatat ggaatttgta aaaggcataa cagttcacgt
    33721 tgaaccatgt actaattgcc aaggaaataa gtgtaataat taattaaaac ttaaaaaaaa
    33781 gtgtaaaaag ttatataatt gttttatgat tataaactct aggggtggtt aaatgagcaa
    33841 gaaaagaaaa ctaaagaaaa aagctgcaat attaatagga gtagttgttt tctttataat
    33901 atactttata tcctttagaa taggaataaa tatggaaaag gataaaagtg aagtgccaaa
    33961 gaaggtagaa aaagaagaag aagataatag gtctttaagg actcaaataa aagagaagaa
    34021 gaaggtttat atatcagata aaaatataga aaacataaaa attgaagaag atatgtggga
    34081 tgaaataaag ttatttttct cagattttaa gaaagtaaga aatactactt cttcttacga
    34141 agcagtttat gaaggttatt ctgatgatgg agttaaattt tctactgacc ttaacttttt
    34201 tagagtatat actgtaaata aagaagaata ttataaagta ccagttagtt ctaaggatgc
    34261 ttttaagaaa cttttagatg agagcatata tacttcgttt gaccttttaa gtcattataa
    34321 agattggaaa gaggttacta taactcatgg aaaagaaatt aaaaaagttc atagatggaa
    34381 atttgatgat ttagcacata agatgatttc aaagagggtt gtaggaaaag ttcaacctga
    34441 gaaaagtaag gaaagaagtg actataattt tactataaat ataaaaggtg ataattttga
    34501 tgtaaaagtt gatacaatgg gaaaagatta tgtaaaagtt acttctaaga aaaatgtatc
    34561 ttactatgag gttcatacaa gtttatttga ttaccttaaa aatgagatat ttgaaattgg
    34621 aaagaaataa gactttaaga aggctatcaa attagatatt tgatagccta aattatttta
    34681 tatattattt ttcaaccaac aagtctgcaa ccttataaat tggaccagca ccagcagtta
    34741 ttactaaatc attatcctta acattttcac gtaagtattt tactatatct tcaaattctt
    34801 ttatatagat ggcatctaca ttgttttgat ataatttctc aaccaagtcc ttagaatgta
    34861 tatctcctgg gtctttttcc ctagcagcat atatatctgt tatgataact ttatcagcag
    34921 catagaatgc ctctgaaaat tcacctaaaa gagattttgt tctagtataa gtatgtggct
    34981 ggaagataca ccataaagtt gattttttta attttttggc agcagctaaa gttgctttta
    35041 actcagttgg atgatgtgca taatcatcta ctactaaagc attcttataa taccctttag
    35101 tttcaaatct tcttccgaca cctttataaa tttttatatt ttttcttata gtctctaagt
    35161 caataccaga aacaagagct gccattatgg cagaagaagc attgtatatg ttatgaagac
    35221 catatacaga aagttcaaat tctcctaaat ctcttccttc atattctatt ctaaatattc
    35281 catgaccact attgtcaaag tgaatatctt ttattacagc atcattgtca gaatctctgc
    35341 cgtattttat aatagtagct ttaacatcat acaatatgtc atcaatattt tcatcatcgc
    35401 catttattat gaaatagcct tctgggggta ataattttcc aaatttgtta aaagaagctt
    35461 tgatttcatc tattccagag aaataatcaa gatggtcttc ttcaacattt aaaactatag
    35521 atatctttgg gttaaaatta aggaaactat ctacatattc acatgcttca gttataaagt
    35581 ggttggaatt acctatctta acattaccac ctatcatact taagttacca ccaactagta
    35641 tagttgggtc taagtctgca tattcaaaga ttgtagataa catagatgtt gtagaagttt
    35701 ttccatgtgt tccagaaact gctatagaat ttttatattc cctcatgatt tggcctaaaa
    35761 aagtcgctct gttcattacc aatttatttt tttctttagc agccacaagt tcttcattgt
    35821 ctggatgaac tgctgctgta taaacaacca tatttacatc atctgatata ttatcttttt
    35881 tctgaccaat ataaatagtt gccccttggt ccctaagttt gtctaatagg tatgattcgt
    35941 ttgaatccga acctgaaact tgataacctt tattgataca tatttcagct aatgcactca
    36001 tgctgatacc gccaattcct ataaagtgta tattcataat aaaaccacct ttctttttaa
    36061 ttaagtccta caatatgaac tatttctgtg gtataataat ataatgttca atattttata
    36121 attaatatgc atataatatt atatgtattt tatttttgaa tataataaaa cataagtaat
    36181 attataccac aataagaatt gttatgcatt agttagttta acgttatata aaggtacttg
    36241 taggaggaaa taataatgaa atttaaaaga acagagagaa ttggagcaat agttaaaata
    36301 ctgtctgata atccaaataa aatatatact ttaagttatt ttacaaatca atttaatgca
    36361 gcaaaatcaa caataagtga agatttatta gttgtaaaaa atgtatttga gaagctacat
    36421 ttaggtaaag ttataactat ttcaggagca gcaggaggag taaaatatat acctaaaact
    36481 tctatagcag aaaatgcaga gttcttaatg gaattatgcg agagaatatg tgacaagtct
    36541 agaatactat ctggtggttt tttatacttg attgacctaa tttacgaccc tacaatagct
    36601 gctaagatag gaaagatatt cgcatcaaat attgagtatg tagatgcaga ttatgttgtg
    36661 acaatggaaa caaaaggtat acctatggca cttatgactg caaaagctat gaatttacct
    36721 ctagttataa taagaaaaga tataaaggta tcagaaggtc ctacacttag catgacatat
    36781 gtgagtggaa acagttctaa agttgaaagt atgagtttgc caagaaaagc tcttaaacca
    36841 gatagtaaag ttataattat agatgatttc atgaggggtg gaggtactat aaaaggaatg
    36901 gtagacctta tgaatgaatt tggtgcagag gttataggta ctggagtatt catatctaca
    36961 acgaatcctt cagaaaaaat ggtaaaagat tatatttcgt tgattcaatt ggatgttaat
    37021 ggagataaaa tagtagtaga accaaactta aagactttta aagatgaata tagaaatgaa
    37081 gatttagacg aagaagaaga ttcagaattt gaatttgaga tagatgaaga ttaataaaaa
    37141 aaatatttag aaaaatatgt aaaaataaaa agaggatatc cctagttgtt catagaatta
    37201 tttaaaaaac agtttatatt aatttattcc agcgataata attttaatat aaattaattt
    37261 ctgtcaaaat caaaggggga tattttggta tgaagataac tgacgtaaga gtaagaaaat
    37321 taacagaaga aggtaagatg aaatgtatag tttcaataac tttcgataat ttgtttgtag
    37381 tgcatgatat aaaagttata gaaggccata atgggctatt tatagcaatg cctagcagaa
    37441 aagtaggcga aggaaatttc agagatatag ctcatccaat aaatgcagaa atgagacaag
    37501 ttttagaaga tgcagtttta caagcgtatc atgaagcatt agttcaatgg gaagtagctg
    37561 ctgaataata ataaatatat aaaatggaga gagcccgaac atttaggctc tttttaaatg
    37621 tagaatttct attaaaatat gtacttttta gtaaaaaagt gctatattat tagtgataat
    37681 gtatatgttg tatacaaatt gttagataaa tgtattataa tgtatattga ttaagagaat
    37741 aatacgaata aaattaaatt agattgaatg gagtgtatca atgaacttta aagctataat
    37801 ccttgctgct ggcaaaggaa caagaatgaa gtctaaatac ccaaaagtta tacataaagt
    37861 atgtggaaaa gaaatggtta accatattat agatgtttca aaaaaatcag gggtaaaaga
    37921 tactgtcgtg attttaggac atgaagctga tgtagttaaa gaaaagcttg cagaagaaat
    37981 tataatagcc atgcaaacag aacaacttgg gacaggacat gctgtaaaga tggctaaaga
    38041 atatattaat gatgaagata caatagttgt actttgtgga gatactccac ttataaaaga
    38101 agagacacta aagagactat ttgaatatca tatagaaaat aaatatcatg ctacagttct
    38161 tacaacaaga gttggaaatc caacaggtta tggtagaata ataagagata agaagggtga
    38221 ccttcttaaa atagtggaac aaaaagatgc aaattcagaa gaaaagatga taagtgagat
    38281 aaactctgga atttattgtt ttaatggaaa gagtcttaga gaagcactag atttgctaaa
    38341 taataacaat tcacaaggtg aatattattt aactgatact gctaaaataa tgagagataa
    38401 aggattaaaa gttggtgcat ttgcaggttc aactattgaa gaacttatgg gcgttaactc
    38461 aagagtagaa ttatcaaagg cagaagaaat tatgagacga agaataaatg aatctcatat
    38521 ggtaaatgga gtaactataa tagacactaa ctctacctat atagaatctg atgttatgat
    38581 aggcaatgat actataatat atccaggtgt tatgttacaa ggaaaaacaa ggataggttc
    38641 agattgtata attggaatga attcatctat aacaaattca gaaataggtg atggtacaga
    38701 aataaaaaat tcaactataa ttgatagtaa ggttggagaa aattctactg ttgggccata
    38761 tgcatactta agaccaaaga gtgacttagg aaataatgta aaaataggcg attttgtaga
    38821 agtaaaaaat gctattatag aagatggctc aaaagcatct cacctttctt atataggaga
    38881 tgcacatgtc ggtaaaaatg taaatattgg atgtggagta gtatttgtaa actatgatgg
    38941 taaaaataaa tttaaatcaa tagtaaaaga caatgcgttt ataggttcaa attctaattt
    39001 agttgcacca gtagttgtag aagaaaaagg atatatagca acaggttcaa caataacaca
    39061 tgatgtccca gatggtgctt tggcaatagc aagagaaaga caagttatca aagaaggatg
    39121 ggtagaaaaa aagaatcaaa aagacgacca aagtaagtaa ttatgtagtt tattattttg
    39181 gctaaaaaat ttagctcgaa attattataa attatattca ttagtatttt aaaattttca
    39241 ggagggttac ataaatgaat actagcggaa gcgagattaa aattattgca ggtaattcat
    39301 cgaaagagtt agcacaaaaa atagctgact atataggtgt atcagtacta gattgtgaag
    39361 taggtacatt tagtgatggt gaaatatgtg taaacatgaa tgagacagta agaggttgtg
    39421 atgtgtttgt agtgcaatct actaacagcc cagtaaatga taacttaatg gagttattaa
    39481 tcttaattga tgcgttaaag agagcatcag caggtagaat aacagcagtt attccttact
    39541 atggatatgc aagacaagac agaaaggcta aggcaagaga tccaatcact gctaaattag
    39601 ttgcaaattt aataactgct gctggagcag atagagtatt aacaatggat ttacatgcag
    39661 ctcaaataca aggatatttt gatataccat tagaccactt actaggtgga acaatattag
    39721 caaattattt taatgaaaag aaaatagaag atttagtagt agtatctcct gacttaggaa
    39781 gtgttactag atcaagaaaa tttgctaata ctttaaatgg agaagttcca atagctataa
    39841 tagataaaag aagaccgaaa gctaatgtat gcgaagttat gaacttaata ggtgatgtta
    39901 aaggtaaaaa tgttatacta ttagacgata tgatagatac agcaggaaca atagttaatg
    39961 ctgcaaatgc acttaaagag tttggagcaa aagatgttta tgcttgttgt actcatggtg
    40021 tgttatcagg tccagctatt gaaagaattg ctaattcaga aataagtgag ttaatagttc
    40081 ttgatactat acaacttcct gaagagaaaa gaatagataa gataaagata aaaacagtag
    40141 ctcctttatt tggagatgct ataagaatga tattctctaa tgaatcagta agtaaattat
    40201 tctaatttat tgtatttatg agagttgttt aaaaggatat aaaccttttg agcaactctt
    40261 ttttgatttt ataattatgg aatgttattt ttttcttgtt agaaaggtat atattattgt
    40321 aaaaagagat tatagtttaa aattatgtat atttaagtaa aataaactta ataaagggca
    40381 tattattcaa aatattatga atataaaaaa tattacaaaa aagtattgaa aaaattcaga
    40441 ttatgtagta ttatgtaaat ataaataaag aaagaaagag gtgtttccaa tgggaaggtt
    40501 agaagatgaa cccctaataa ttaaatgatt cagtcacaat ctaaagtgaa tataaaaatc
    40561 tattgaaagt ttatttataa gaattgaaaa tagtatctat tctatcttta gtgtttgtga
    40621 gagggttctg gattatataa gattactgta aagaagtaat tttgcacaat aagcatatct
    40681 gtaaatgaag agtttcgttt atataatgat tgtggctgaa tcataactat aatagataga
    40741 atgtttataa tagatgattt tataaaatac tatattgtaa tagcttttag ctttcaaata
    40801 aatattagca aaggatggaa atgacaatga taaaactaaa tgataattta taaatatctc
    40861 cttacgatat ataataattg tagggagatg tttctttttt ttgtagagta taatttttct
    40921 agaaattctg tttcaatatg taaaaagttg gtatatataa atagtataaa cagtgttgct
    40981 aaaatatggt agatattata taatataaat ggatatgtat atacattatg agttatataa
    41041 tttgaattta ataaaataga aacgttgatt tatgttctgt aatgtgggca ccttggagca
    41101 tattgagtta gtggtgcaac cggctatgaa attgtattta tttataaata ctattatttt
    41161 catagctttt tttatttaaa aaatggttat atgttagaaa taaatttggt tagtacgttt
    41221 aaataatttt acttagattt ttaaccagat atattataag taagtattaa ttaaaaaatt
    41281 taagggggaa taaaaatgaa gttaaaaaag aataaaaaag gtttcacttt agtggaatta
    41341 ttggtagtaa ttgcaattat aggtatatta gcagtagtgg cagttccagc tttatttagt
    41401 aatataaaca aggctaaggt agcaagtgtt gagtctgatt atagctcagt taagagtgca
    41461 gcattatctt attattcaga tactaataag ataccagtta caacaactga taaagctggt
    41521 ttaactattt tagagactta tatggagtct ttgcctgata aagctgatat aggtggaaaa
    41581 tataaattga ttaaagttgg taataaattg gtattacaga taggtacaga tactgaagga
    41641 gttacattaa cagaagcaca atcagcaaaa ttattgagtg atataggtaa agataaaata
    41701 tatacaggtg ttacaggaga taattttgga gacgaattaa cagatactac aaaaatagat
    41761 aataaagctc tatatatagt acttatagat aatactgtga tggactcaac aaaataatta
    41821 aaactatatt tttataaaga atattgtaaa tttaatgaaa aatataatat aaaagacagg
    41881 aagaaaatct tcctgttttt ttatactatt ttctttaatg ttcaattatg aatgatgata
    41941 taattgagat agtaaaggag tgaaacctgt ggctaaaaaa gttagaatag gagacaaact
    42001 ggtcgaaaaa ggatatataa cagaagaaca gcttaaatgg gcactatcag aacaaaaaaa
    42061 cagtggaaaa agactaggag aatttctagt acaagaaggg cttatagata gtaacttact
    42121 aataagtgta ttaaaagaac tcttggatat agaaagtata tttttagaag ggacagaaat
    42181 agacacttta gctacaaaaa tggttccaga aaatatatgc aaaagatata ctgtatttcc
    42241 atttaaaatt gatggaaata aaatatgtct agccatgtca gacccacaag atagagaagc
    42301 agttcaagat gtaaggcgta tgtctggtaa agacgtagag atatttatat caagtacaga
    42361 agatataaac aaagcaatag gtcatgctta tgcacattca gaaataaata aggcaatgac
    42421 tgagtacaac aaaaatagaa ctggtggagt aagagaaaca gtaatacttg aagaagatgt
    42481 aaatgctgct ccaatagtac gattagtaaa taacatacta gaaaatgctg ttagaatgga
    42541 agcaagtgat atacatatag aacaaagtga aaattacatg cgtgttaggt ttaggataga
    42601 tggaatgtta agagaatata tgaggatgaa ctcagcacca tataaggcag taatcagccg
    42661 tatcaagata atgtcagata taaatatatc tgaaaagaga ataccacagg atggtagaat
    42721 ctatttaaaa gtggataata aacccataga ttttagggtt tctacaatgc caactaacag
    42781 ggatgaaaag atagctatga gggttcttga taagagtaat tttatggtta gtaaagaagt
    42841 actaggaata gacgaacatg gctctaagat atatgatgaa ttgataaaca ctccttatgg
    42901 tcttatattg gttgttggcc ctactggaag tgggaaaacc actacattgt attcaatgtt
    42961 aaatcaacta aatacagaaa atagaaatct attaacaata gaagacccta tagagtatga
    43021 attacctggt gtaaatcaat ctcaaataaa tgaaaaggca gggcttactt tcgccagcgg
    43081 acttagagct tttatgcgtc aagacccaga tataataatg gttggagaga taagagatac
    43141 tgagacagca gaaatagcaa ttagagcttc tcttacagga catttagttt taagtacatt
    43201 acatgcaaat acagcagttg gagcaatttc acgtctttta gatatggatg tggaaagttt
    43261 tttaataact tcatctgttt taggtgtaat atctcaaaga cttacacgaa aaatatgtga
    43321 gcattgtaag gtttcatatg aagcagatat tggagaaaaa aaggctcttg gtattgatgt
    43381 aaatgaatca gtaactattt atagaggaaa aggttgtgaa aggtgtaata atactggata
    43441 taaaggacgt ctaggtatat ttgaaatgct tgaaattact ccagaaataa aagagttaat
    43501 agattcatct gcaaatcaaa gagaaatttt aaagatggct agaaaacaag gtatggttag
    43561 cttaaaagag gatatagtta aaaaagtttt aaatggaaag acaacagtag aagaaatgat
    43621 tagaataatt ttgatgactg attaaaggat gatattatga aaagttttaa atacacagta
    43681 ataaaagagg acggaaaaaa gcaaactgat gtgattgaag ccaacgattt taatgaagct
    43741 agaaatcttc ttagaaagag aaatcttaga gttattgaaa tcaaagaaag caaaaataaa
    43801 aatataagat tatctagtag aaagagaaaa aaagacttag gagctgacca gataagccac
    43861 ttttgtagac aatttgctat tatagtatct tctggtataa atagtatttc tggattagaa
    43921 acattagcta gaaggtcaac taacattaca ttaagagaag aaattaatcg tatagtagca
    43981 gaaataaaag taggttctac tattgcagat tcaatgcttt catcaaaatc aaagtttcca
    44041 aagttacttg gtgctatggt agctacaggt gaagctacag gtaaattaga tgaagtatta
    44101 aaaagtatgg cttcatttta tgcaagtgaa cacagggtta aacaaaagct tagaaatgct
    44161 gcaacatatc cattaatagt tttgatttct tcatttataa tgatttttat atttacaaca
    44221 tttatggtgc ctaagatgat taattcaatt acaacagtag gagcaactct tccacttata
    44281 acaagaattg ttatggggtt tggtatgttt atgaagaagt tttggataat agtactttta
    44341 gttattatat tatttatcta tcagtttaaa aaatatctaa aaacgtcagt tggaagggct
    44401 cataaagata gagttataaa taagatacct gttttaggaa aaggaataaa ttgtatggta
    44461 gctacacgat tttcaagagc attgtatcta tttgtatcaa caggataccc attagttcaa
    44521 gggcttgatt atattataga tagtgtaaat aacactatgg cagagaagat tttagcatca
    44581 gctaaagatg gaataacaag aggagaaggc ttagcagaaa atttagagag atatacgtat
    44641 tttgactctg tgttggtaca gatgatagct ataggggagc agacagggga attagagaat
    44701 ataagtagac agatggcaga attttatgag tatgaatccg agatttattt aaataggatg
    44761 gcctcaatga tagagcctgt tttaataatt gcagttggta ttatagttgc gatactggtt
    44821 gtatcaatat tcatgccaat gcttagtata tatgatgcca tgtaggaggg aaaggacaaa
    44881 tgaacaaggt ttatgtatca tattatggcg attctatttc catagttgag ggcagatata
    44941 aacacgataa ggataagttt tatgtaaaaa attttaatgt gttttcaata gaggatattg
    45001 cacaagattt ttctaaggaa aagtatgctc tattgagata cgcattagaa aatgttaagt
    45061 taaaatcaag acatgtagtt ttttgtttaa atacaagaga tgtaatttta aaaccccaaa
    45121 aacttcctaa tatggacaaa aaggatttag acggatttat gaagctagag atggacgaaa
    45181 tgatggcttt ggagtcagat gaatatgtat tttcgtatga agtatctagt gaaacacctg
    45241 ataattcaga tggcgatgaa tcaagcttaa atcttattat tggagctata caaaaggaag
    45301 aggtaaatgc aattgtagag ataatgcatg aatttgattt agtattagat agaatagata
    45361 cattatcaac agcttactta agaatgctta aaaatttaga ctacaatgat ataatggtaa
    45421 ccaatataag tgacaatagc acaattataa atatatataa gaaagatact ttatttatta
    45481 gtgataatat acctataaag ataactgcta aagatatgga catgcaatta ttaaatgtag
    45541 tagaagaatc aaaaggtctt atgaattact attcgtcaag aaactttggt aaggttacgg
    45601 atactatatt aatattagga aaaagatatt ccaataaatt aatttatgaa acttttagct
    45661 ctgcattttc aagctatgtt tctcaaggcc ttactgagat aatggatata tctgatatga
    45721 taactggaga tatagatgaa gatgatataa attcaattgt agagctttta ggaagtatga
    45781 tagaacctaa agggaaaaga gattttgaaa atataaactt tttaccaatg gatatactta
    45841 gaaaacagat aaaagctaaa caaatgaaaa acatactgaa aattgctcca atagttattt
    45901 tgattcttgc tataccttat ttatgtttaa ttttttcaaa taatttagca aataaggaac
    45961 ttgaaaatgt taatggtgaa ataagaaata ttgaatctgc atattatcag attggaaata
    46021 tagaaaaaat gatacagagt aaaagcgaag aaataaaggt atatgatatg ttaattggga
    46081 aaaaggtaaa gtgggaaaat ttattggatg atatagataa aaatactcca agtaaagttg
    46141 aactaacaag tttatcaaca acttatcaaa atcttggaac taataataaa gctgataatt
    46201 caaaaagtaa taagactaca acagataaat caagtaacaa tgaaacaaaa tcaagtacga
    46261 aaagtgatag taatggagat aaaacagata atgatttaaa tgaagataag actacaaatg
    46321 tgtcagaagc attaactgga aatagtgaga ctaatagtaa taacacggat aaagatgata
    46381 aaactccatt gtatgataag ataccaaatg ttataaatat agaaggaaat gctaaagatt
    46441 cctcttatgt aggtcaattt ttatataaat taaagggatt atactatttc aaggatgtta
    46501 aattacatag cataaaaagt aatgcagaag attctaacta tacatttagt atgacattat
    46561 acctgaaaga aggtgtatta actaatgaat aaagatatat ttaaaaaaga tataaaagat
    46621 atatttaaaa tggaagttag tctaaaaggg attcttataa aggttataat tttattagct
    46681 attttatcat atggttattt tggactttac cctcaatatc agaaatatat tgctacaaaa
    46741 aattctgtaa aacaggctac tgaaaaatta aatatatata aagagaaaac tgcacttatg
    46801 ccaaaatatg aaaaacaact tgaggaagtt gaaagtgaat taaaagaaaa aagcaaaaat
    46861 cttttatatg atatgcaaga tggtcttttt ttaataggtt tagataaaaa aatgaaggct
    46921 tataatattg acttaaccag ttatgacgtt ggaaatgtta aagaatacga acatttttat
    46981 ggtatatcaa caacaataag tgttagaggt gattatagaa atgtcaaaaa attaatggct
    47041 tatttagaag aacaaaaaaa tgttactcaa atactagatt atacaatgac ttcatatata
    47101 gaaccagaaa aagaagagga agaaacaaca aacaataagg atttaattaa aaatgtgaac
    47161 ataaataaag atacaaaaat atactgggtg gaaggcgaaa ataagtggca tttatataag
    47221 gattgtccat tgataaaaga gggagagatt ataaaaaatg acaaactttt agataggtct
    47281 actaattatc tatgtaatac ttgtgtactt agagctgcaa cagaaaaaga agcaaaaaaa
    47341 gaagtcgtag ctccaaaggc agaaggtgta gtagatgcta catttaaatt tataatgtac
    47401 actaaagaga atactacaat gatacttgat acagataatc catcttcatg gagaccagga
    47461 aaatataatc catttaagtc tagtgcgaaa taaagctaag taagctttaa agataggtga
    47521 tgagtttgta caaaaataat gaaaaaggtc ttactttatt agaagtaata atagctgttt
    47581 ttatactgac aatagtttta agtatttctt ataaagtgtt caatggtata acatcagcag
    47641 taaaaaagca acagattatt acagatgctc aagtaaatat taatctaatt aataagtatc
    47701 taaatagaga tttggaaaac tgtaaagagc taactaaaac tggttcaggt aataattatg
    47761 aatacaatat agagatgcca gataatgttg taaaatatga agtttctata gaaactaaaa
    47821 aaaatacaga ggtatattct gtaacgagaa tccaaaacaa tacaattgat acagaaaatg
    47881 aagttagaga agagataatc tataatcaac cactagttca aaataataaa gaaatgaagg
    47941 aaacaccatt taaaatagaa aagcaaactg gtaaatctat ttatactgta agtatatact
    48001 ataatgaatc ggtgcaagaa agtcataaaa atagcaattt aaataacaaa acttatacat
    48061 ttgatgttat gtcaagaata gggtaataag aatttattaa aaaataagta ggttgataaa
    48121 atatgagtaa aaagaatagt gaaagaggat tttccttaat tgaagtgtta gtagctatgg
    48181 ctattatggg aattgtacta tttgcatttt ttaatattat caatactaat aacaaagcaa
    48241 acactaaaaa tgacacagat ataacttcct taaactatgt ccaaagtgaa atagaaaatc
    48301 taagagaaaa gataaaaagt ggagaatttg attttgatag tttagataaa ctagaagatg
    48361 gaactgtcgt atatgaaaaa ttaatagata aatcaaaaaa agttgtatat gataaagttc
    48421 ttagtgaagg tgatgtgagc ttatatgata ctccatatga aaagattaca acaataaaag
    48481 atgaagatgg taatttaatc gacaaaggaa atattacaaa taagataaag actattgtag
    48541 aagataaaag tggtcagata tataaaatag ctgtaactgg gaaaagcatg aatgactatt
    48601 caagtaaaaa agaggttaaa attgtaactg aaatatttaa agataaataa ttttacttgg
    48661 gggtgagatt ttgaggaagt ggaataaatt taaaagtgaa agaggggcag ctttagtttt
    48721 agttttggtt gtagtagcat tgctttctat tgttggattg atattttcaa atcagatagc
    48781 taatagaata aaatctacaa agactactaa tgaaggtata caagcaaagt atttagcaga
    48841 gacatgtgtg gaaaatagta ttgataaagc atatgaaaaa ttgtatgatg agcttgagaa
    48901 aatggataat gagtttaaaa gtgaaaatca agaaaaatcc atatctaggt caaaattaag
    48961 aaatatatca gatgaagatt ttaataatca agatgaggaa aatattgaag cagaaagact
    49021 tggatatatg aataatataa atttctattt aaataaagct tcttcagact tggaaaaagc
    49081 tagtatggaa ttgaaaaagc tatatgattt agatatgttg gactatagag acattgaata
    49141 tgtagatgca aatattattt cacatagaaa ttctatctta gaaatttgca agaattatac
    49201 ttctggagat attagtaaaa taaatgaata tattctgaaa gaagatatag actcaactac
    49261 attgattgag gcaaagttag tgaataatga tattctttta aaaaagtttt tagaagaaaa
    49321 taaaattgaa aatgaacatt taaattcagc cttttcacat acttataagg cattagataa
    49381 catttctttg gcgatgcaaa atatgattga gtatagacat acgtttcata tagatgagcc
    49441 aaaagtagaa gtatctaatg ggattccaga ctctcaacaa tattatgaac taatacaaaa
    49501 tcctataata aatagtatgg agtatagctg gaatagtaaa tgggatacat tagaaaacct
    49561 attggaaatt ttaccaaatc aaactcaagg atttaattct ttaagagttc atttgagaaa
    49621 taatgtaaga aaatttgaga agttaagcga taatatatct agtggtaaaa aaaatacagc
    49681 aaaaaatttc ttaaagtata aagagttatt gtatgaaata tcagaccaat gtaatcaact
    49741 aaaaagtatg tcttatgaga aaatacctgt taaatatgat aacatggctt tgataacaac
    49801 ttttgactat atacaaaatg agcttttagc agagataaaa tgcagattaa aagaattaaa
    49861 accacaagaa attgataaaa cagaaggtat tactataaaa ataccttttt ataaggctga
    49921 ctacgacatg acaaaagaag gatggcctaa attaaaagaa aatggttcag gggcggagtt
    49981 aagcttaatg gtaactggtg ataaaaatgg aataaaagaa gtagaagtta cagatggtaa
    50041 aaaaaatatt attggtttag gtgttgaaga aaattcaaat tcaaagtata aggttgacgc
    50101 cattgtaaac tttaatctaa atatagatac aaatgtagtt ggaaattatg atataaaaga
    50161 taaaattttg attaatcatg atataagttc atacaaaaaa gtaaactaaa tataagaagc
    50221 aacttagtat aaataaataa taaaataatt ttattattta ctactaaaaa aataagtaaa
    50281 ctaatagaag atatggagag taaatattat gtatatttat gatttattag agcaaggtat
    50341 aagactaaac gcatcagata tacacataac agttggaaca aatcctgtag ctagagtaaa
    50401 aggtggattt gtaaaactaa gtgaacaaat attgacatca gaagtaacaa tgcaaatggc
    50461 aaaagacata gcaggggaat ctatgtttaa agtaatagaa gaacatggag aagctgattt
    50521 ttcagcttct ctaaaaacag gagaaagatt tagggtaaat gcatataggc aaaaaggaaa
    50581 ttacgcaata gcaataagga ctataacagc agaaattcca acatttgaaa aattaggcct
    50641 tccagagagt ataaaatctt ttactgaaaa gcataaagga ttagttttag ttacaggtcc
    50701 aacaggaagt ggtaaaagta caacacttgc aagtatgata aatataataa atgaaaaaca
    50761 acaaaaacat ataattacac ttgaagaccc gatagaatat gtacatcatc ataaacaaag
    50821 tttagtaaat caaagagaag taggaactga tacagaaagt tttcattcag cccttagagc
    50881 tattttgcgt caggacccag atgtaatact aataggagag atgagagacc ctgaaactgt
    50941 ttcaatagca ttaacagcag cagaaacagg tcatttagta ttttcaacac tacatacagt
    51001 aggggcagct aagacaattg atagaatagt agacatgttt caaccatccc aacagcaaca
    51061 aataaaaaca cagttatcta cagtttgtga aggtgtggta tcacagcagt tacttcctac
    51121 agcagatggg aaaggcagaa tagcagcaat tgaattgatg tttgctactc cagcaataaa
    51181 aaacctgata agagaaggca aaacatatca aattccaaac atgatacaaa ctggagttaa
    51241 agtaggtatg aaaactatgg accaagattt aatggaactt tataaaaatg gaaagattac
    51301 aaaagatatg gcacttagta ggtgtactga ccaagagttt atgacaagaa tgataggtgg
    51361 agtaaactac aatggatatt ataatcgtta atctatatgt atttatagtg ggtatagtgt
    51421 ttggaagttt ctttaacgtc tgtattttta ggattccaga aggtatatct atttctagac
    51481 caccttctca ttgtatgagt tgtaatacta gactaaagcc aatagactta ataccagtgc
    51541 taagctattt aatgagtggc agaaagtgta gatattgtta tgagaaaatt tctagtagat
    51601 atgctatagt ggaattaatt acaggacttt tatttttaac agtatttatg ttatatggaa
    51661 tatctacatc aactatttat tatttggttc ttgtgtcttt actgattata attacattta
    51721 tagatattga ccactttata ataccagata aaatattggt ttttggagct atattttcta
    51781 tgatttttaa ccttttattt aaagaaattc cagttaagga ttcaattcta ggtgttttta
    51841 tttgtggagg aagtgtctgg attatagttc ttttgattga atttgtaata aaaaaagagt
    51901 gtatgggtgg aggagatatc aaactatttg caatgctagg tttttacatg ggtgttaaaa
    51961 atggtctttt gacagcctta ctaagcgttt atgtgggagc agtctatgga atctgtgtaa
    52021 taattataag tagaataaaa ggaaaggaat ataattctgt tataccatat ggacctttta
    52081 tatctattgg agcattaata acaatcttat ctggaaagca attacttgaa ctttattttt
    52141 ctatggttat atagatgata gtcaagttaa ataaagtttt aatttaaaaa aatgaattaa
    52201 agtatttaat aaggacaata ttattattaa ccctattact aaagtgctac aaactatgga
    52261 gtatataata ataatttcct tattttttgc acatttgata aataaatctc tatgtgtttt
    52321 ttgtgataga gaaaagaaaa atttatataa tacgtttttt atttacataa taactgtagt
    52381 tttatctgta ttaatatatc taaaatttgg gattagtgta gaatgtgtac aatatatggt
    52441 tttaattcca tttttaataa ttatatccat tgtagattac tacacaatat atgtatatga
    52501 tataacaata gttagtggta taatagttca aggtataatt tttttggcct ttttcaatat
    52561 agttcaattt gattatataa atcatatatt tggactattg ataggcttta ttatacctta
    52621 tattattgct aaactaacaa aaggattagg ctatggagat gtaggtctct atagcttatg
    52681 ttgctttgca ttgggaaata attatgggtt ttatgtgata gttttatcat ttattttagc
    52741 gtttttgttt tttattattt tatacataac taaaatattt aaagatgtag atataagaaa
    52801 gcaaattcct tttacacctt ttatttcctt gtcaacaatt ttaataatat ttacagagta
    52861 taatatattt aatttttatt ataatttgtt atacaaattt atataacagg aggtaagaaa
    52921 tgtacgtagt agtagggtta ggtaatccag gaaagaagta tgagaagact agacataacg
    52981 ttggatttga tgttatagat attcttgcta aagaatacaa tattagtgtt actaaaataa
    53041 agcataaagc tttgattggt gaagggagag taggtactga aaaagtatta ttagttaaac
    53101 cacaaactta tatgaattta agtggagaaa cattaataga tatatataaa tattataagg
    53161 ttgatttaag taatatagta gtagtatatg atgatataga tttagaagtt ggaaagatac
    53221 gtataagaaa aaaaggaagt ggaggaactc ataatggcat gaaatctata actaagtgcc
    53281 taggttcaaa tgactttcca agagtgagag taggtgtatc taaaccagaa gctggtcaag
    53341 acttagcaga ctttgtactt tctagattta gaaaagaaga atctgataac ataaatgaag
    53401 cattagaaaa agcggcagat gctattgact caataataag agaaaatata gatatgtcta
    53461 tgaacaaata taatggatag tgagagggag atttaaatga atgatgtttt tttatatcct
    53521 ttgcaaaatt caaaagagta taaagatata ataaactgta taaaaaatac aaaaggctca
    53581 ttattggtta atggactttt accagtacaa aaacctcata tatcgtattc tatatttaat
    53641 gatttatcac gacaaatgat ttttataacg agtagtgatt tagaagcaaa gaaagtatac
    53701 gaagatttaa gtttttatat ggaagataaa gttgaatatt taggttttca agatatttat
    53761 ttttatcact tggatgcaaa ggacagaaac gaagaagcga agaaattaaa agtattgtta
    53821 aaactagcaa ataaagaaaa aatcatttta gtaacttcta ttgaggcagt tttaagaaaa
    53881 tacataccaa aacaagtttt gctcgatagt gtatctcatt ataaggtggg agatagctta
    53941 gacttagaaa agttaactga gaaattagtt tcattgggat atgaaagagt ttcaaaaata
    54001 gaggggtttg gtcaatttag tataagaggt ggaattattg atgtattttc acttgaatat
    54061 acaaatccaa ttcgtatgga attatttgat gatgagatag attccatacg aacttttgac
    54121 gtatattcac aaaaatcaat tgataaattg caacaattct ctataactcc atctagggaa
    54181 tttatttatc ccgaaaaaac tacagatgca ttagttaaat taaaaaaaga aacaactaaa
    54241 aatactgatg aaggtacatt tcaaaatata gattatatat caagtaagac ttattttgaa
    54301 ggtgtagaga actatataga ttatatttat ccagaagaaa acaaaagtat atttacttat
    54361 ttagcagatg atgcaatagt atttattaat gatataacga gacttaaaga aagatgcgaa
    54421 aattatataa atgagtttag agaaaattat aaattaaatc ttgaaagagg tctggctatt
    54481 aaaaaccaag gtaaattgtt atatcactat acagatttag agtacttagt aaaagacaag
    54541 tctgtggtat taaactcgct attgccaaag tctataaata acttttctat aaaatctata
    54601 ataaattttg aaagtagaga agttcctaca tttaatggaa aggtagattt gcttgtagaa
    54661 gaattaaatc ggctgaaata taatggatac aaaataattt tagcaacaaa taccttagaa
    54721 agagcaaata aattaggaaa agatttattg gacaaaggct tagaaacgac catttctaaa
    54781 gatagagata ttgaaattaa atcttcacaa gttataattg tacctgcaca cataaacagt
    54841 ggttttcagt ataaatctat aaagtttgtt gttataacag ataacgaaat gataggtgtt
    54901 tataaaagag cgtctaagac tagcaataaa aaagttaaaa agggaaagaa aatagaatcc
    54961 tttttagatt taagtgttgg agactatgta gtccatgaaa atagtggtgt aggtagatac
    55021 acaggaattg aacaaattac agtcaatgct attaaaaaag attatatgaa aatagtatat
    55081 caaggtggag ataatctata tgtgccaata gaccagatgg acaaagtaca aaaatatatt
    55141 ggtgcagaag tagaaaaagt taagttaaat aagcttggaa caaatgagtg gacgaaagca
    55201 aaagcaaaag ttaaaaaaga aattgaagat atgacaaaag acttgattga gctttatgca
    55261 aaacgtgaaa aaatacaagg gtataagttt tcaaaagata caccttggca ggcagagttt
    55321 gaatcgttgt ttccatatca agaaacagaa gaccaattaa aggctataga agaaactaaa
    55381 aaagatatgg aatcaagtaa ggtaatggat aggctagtat gtggagatgt tggttatgga
    55441 aaaacagagg tagctattag aagtattttt aaagcttgta tggaccaaaa acaagttgct
    55501 gtattggttc caacaacaat attggcccaa caacattata atacattcaa agagaggttt
    55561 gaaaattatc ctttgagagt agaagtttta agtaggttta aaacaccaaa acaacaaaaa
    55621 cagattattg aagatgctaa aaaaggatta gtagatattt taattggaac acatagaata
    55681 atatccaaag atataaattt accaaactta ggtcttgtag tcattgatga agaacaaaga
    55741 tttggagtaa aacataaaga agctttaaag aaaattaaat ctacagtaga tgtacttaca
    55801 ctttcagcaa ctcctatacc aagaacatta catatgtctt taagtggaat aagagacatg
    55861 agtgttatag aggaacctcc acaagaaaga catcctgtaa taacatatgt tacagagagt
    55921 aaggaaagcg taattcaaga tgagatagag agagaattaa gtagaggtgg acaggtattt
    55981 tttgtataca atagagttga acacattgaa gaaatggcaa gtatgattca aaaactcgta
    56041 ccagatgcta gagtggctgt tgcacatgga agaatgactt ctaaatcatt agaaaatata
    56101 atcctaggat ttttaaataa agattatgat gtactagtct gtactacaat aattgaaact
    56161 ggtatggaca tatcaaatgc aaatacaatg attatatatg atgcagataa aatgggattg
    56221 gctcaacttt accaattgag aggtagggtt ggaagaagtt caagacaagg atatgcatat
    56281 cttttgtatg aaaaagataa aactttaagt gagatagcgg agaagagatt aaaagctatc
    56341 agagaattta cagagtttgg ttctggtttt aaaatagcaa tgagagacct tgaaataaga
    56401 ggagcaggaa atattttagg ttctcaacaa catggtcata tggcagttat aggttatgat
    56461 ttgtacgtta aaatgcttaa tgatgcaatt aaaaaagtca aaggtgaacc tattgtggag
    56521 gaaattgatg ttgaaataga tttaagtgta aatgcataca ttccagataa ttatatcaaa
    56581 gatgagttga ttaaaattga aatgtataag aaaatagctt ctattgaaaa caaagaagac
    56641 atgttggata tacaagaaga acttgaggat agattctctg atatacctaa accagtccag
    56701 acacttctta caatagctta tataaaatcg ttatgtaaga tattaaaaat agaaaagata
    56761 agacaattaa aagatgaaat attattagtt ccaataacaa agtatagaac taaacagaaa
    56821 ataggttata atatagttac agaattagaa gaattattag aaaagatgtg caaggtaaaa
    56881 taacttgtaa atttaagaag ggatgtttta aatttgaaaa aggtaataac attggtaata
    56941 gctatgattt tggtagtatc tgtgactgct tgcagttcta gtaaaggaga aactgtggca
    57001 acagttgaag gtacaaagat atcatcagat gaatttaaaa aaactatagc tctttataaa
    57061 gattctatgg aacaaactta tggaaaagat atatgggata aggaagttga aaagggtgta
    57121 aaatacaagg acaagtttaa agatttaatt cttgaccaac ttataactac agaagtaata
    57181 tattctcaag ctaaaaaaga taacttactt cctaaaaaag aggatgttga aaaaagcttt
    57241 aaagaattaa aagacgctat gggtaaagat gaaaaatata aagaacaact taagaaatta
    57301 ggtatagatg atgagttttt aagagaccaa caagagaaag atttagcaat gcaaaactat
    57361 caaagtaact ttgctaaaaa gactaaaata tcagatgaag aaatgaaaaa atactatgat
    57421 acacataaag atgaatttaa aaaagatgag gtagaagctt ctcatatttt attgaaaaca
    57481 gtagatgata ataacaagcc actttcagat aaggaaaaag ctgaagctaa gaaaaaggct
    57541 gaagaggctc ttaaagaagt taaatcaggt gaggattttg caaaagtagc aaaaaaatat
    57601 tcacaagata cttcagcaag tgatggtggt aaattaggat tcttttcaag gggtcaaatg
    57661 gttgctgaat ttgaagatgc tgctttttct atgaaaaagg gtgaagtatc tgatttagtt
    57721 gaaactcaat atggatacca cataattaaa gtgacagaca gaataaatga gcaaacatca
    57781 tttgaagatg ctaaagaaac tataaaagac caactgttga aaaataaata tcaagaacaa
    57841 atagaaaaat taactaaaga agctaaggta gaaaaagatg aaaaagtaat aaataaaatc
    57901 acaatctaag acttaaaaaa agagtaatat gcttaaaaaa gctatatctg gataagatat
    57961 agcttttttt gtatattttt cttaacaatg gtaaaaataa acttaaagta atttatggag
    58021 ggattaaaaa atatgaaagc aacaggtata gttagaagaa tagatgatct tggaagggta
    58081 gttatcccaa aagagataag aaagacatta agaattagag aaggagaccc tttagagata
    58141 ttcacagcaa aagatggaga agtcatactt aaaaaatact ctccaatagg tgaattaaat
    58201 gaattttcgc aagaatatgc tgaaacatta gctgaagtac taggatatgg agtgttgata
    58261 acagacttag attctataat agctgtgtct aagcttccta aaaaagatta taaagaaaaa
    58321 agtataagtg atgagttaga agcaataata agtgaaagaa cagtaaaata ttcaaaagat
    58381 aaaaacattt taccattatt caaggaagac caaaaagaat atactagtca agttatcatg
    58441 cctataatta gtgcatctgg tgactgtata ggttcaattg tagttgtttc aaaggataaa
    58501 gatacaattg cagaaagtga agaaaaatct ttaaaaattg ctgctaactt tttaggaaaa
    58561 caagttcaat aattttatca taaagacctt ttacatgaag gtcttttgaa gtttttgtgt
    58621 aaaatattga atatagcatt aaaaatatgg tatattaaag tttgttatat aaacagtatg
    58681 gaggttatgt tatgaacaat aacaaaaata aagactcctt tctaaaagga gcccttatac
    58741 ttgggctagc aggtgtaatc gtaaaaataa tgggtggatt ttttaggatt ccccttggaa
    58801 acatgattgg ttcagaagga atgggatact atcaggctgc ttatcctgtg tatactctat
    58861 ttttaacatt agctactgct ggatttccaa cagctgtggc aaaattggtt tcggaaaaag
    58921 ttgcaattgg aaattttaaa ggtgcaaata agatatttaa ggtatcacat acagttttat
    58981 tcattaccgg tataatatca ttttgtatat tattttttgg agccgactac atagtaacaa
    59041 acgtaatgaa aaatccaggt gcgttatact ctatgaaagc cattgctcca gcattactat
    59101 ttgttcctgc aatgtctgca tatagaggat attttcaagg gagacaagat atgactaaga
    59161 ttgctgtgtc tcaagttgca gagcaatttt ttagagttgt attaggtctt actttggctt
    59221 acttgcttat gaagagtcta ggtcagactt ttggtgcagc aggtgctata tcaggggcta
    59281 caataggttc aatagcatct atgctttatt tggtgtttgc ttatatgcta gggagaaaag
    59341 agcgtagagc agagatagat gcaagccaaa gatttaaaga tgaaagagtg tcttatattt
    59401 ttaaaaagct tttaactgtt gccattccta taacaatagg agcatcagta atgccacttg
    59461 taaatatgat agataatgtt attgtaataa gaagacttat ggaagcaggc tttacataca
    59521 aagtggcaaa ttccatgttt ggtcaattaa caggtatggc aatggccaca ataaatttac
    59581 cagcagtcat aacaactgca atgagtatga gcttagttcc agcaatttct aaagcatatg
    59641 cattaggaaa taagtcaaaa gctagaaaag atactaagag tgcagttaag gtaactttac
    59701 ttatagtatt gccttgtgcg tttggtatgg cttctttagc aataccaata atgggtcttt
    59761 tatttccaca tgaaccatct tctgtaggaa ctattttgtt tactcttact ccatgtgtat
    59821 tatttttagg attaatacag acattgactg gtataattca gggaatggga aaaccaatag
    59881 tacctgtaat tgctttatgt gttggtatgc tatgtaaaat tgtaataagt tatactttaa
    59941 caggtatacc agatattaat gtactgggtt cagcatttgg tacagttaca gcatactttg
    60001 tggcagccat gattaactta ctatatgtta aaaaacacat gaatgtaaac ttttctaaaa
    60061 aagaatttat aataaagcca tttataacag ttatgacaat gtttataatg gttaagttga
    60121 gttatggtgc tttagttgga ttcttaggaa acagcatatc tacaataatt gcaatatgtg
    60181 ttggtggaat tgtatatgta gtagtaatac ttggaatagg tggaattaaa aaagaagaga
    60241 ttctaactat gccaaaaggt gataaattat ataaattgtt aaaaaaagtt aaacttataa
    60301 gataatatag gaatatatat gcaaataaag gaaatactat acaaatagta aatctcctta
    60361 tttgtataat aatataaaaa taaagtgaaa tttgctaata ggagtgatgt tatgggtaag
    60421 ataagtattg taggtcttgg accaggagac tattctctta taagccaagg tgtgttagaa
    60481 tctttaataa atagccctaa agtttttttt agaacagaga aacatccaat aattgataaa
    60541 cttaaagaaa ctatacaata cacttcttta gactactttt atgaaaaaga ggaaaatttt
    60601 gataatgttt atttgaaaat atctgaattt atcatagaag aatcagcaaa gggtgaccta
    60661 gtgtatgctg ttcctggtca tccaagagtt gcagagaaga ctgtaagtat aatagagaga
    60721 ttagccaatg aaaagcacat agcagtagaa atattgcctt ctatgagctt tgtagacgct
    60781 atgtttaact atttggctat agacccatct gagggattta aactggtaga tgcttttgaa
    60841 atagaaaatt catatataga cttggataca agtatgataa taactcaggt atatgataaa
    60901 tttatagcat ctaatgtaaa gttaaaactt atggaacatt atgactataa tcaagaagta
    60961 tgtatagtaa atggagctgg tgtcaaagat ttagaaagta aaaagtttat aaagctttgt
    61021 gaattagaca gaaatgaaaa cttttttgac tatttaacta gcttatatat acccaaaagt
    61081 tctaaaaaga tgtataatac agtacatgac ttagaaataa tagttaatac acttagaagt
    61141 ccatctggat gtgaatggga taaaaaacaa acacatcaat cattaaaaaa ttctgttata
    61201 gaggaagcat atgaactttg taatgcaatt gataataatg atatagatga aatggtagaa
    61261 gagttaggtg atgttctttt acaggttata ttccattgtc agataggaaa tgaggagggc
    61321 tactttgatt taaaagaagt agtaaatggc atatgtaaaa aattaattca tagacatcct
    61381 catatattta gtggtgcaaa attagatatg tctaagtttg agaaaacttg ggaagaatta
    61441 aagaaagaag agaaaggcga aagtactata acagatggcc ttagaaggat accaaaacat
    61501 ttaccagctc ttatgaaggc tagtaaagtt caacataaag cagcacttgt cggctttgat
    61561 tgggacaatg tagaagatgt atttaaaaaa attgaagaag aatatcagga attacttgac
    61621 gaacacaggg tcgggaatat aaaatacata aaggaagagc taggcgattt attattttcg
    61681 atagtaaatc tagctagatt tttagacata gactcaggag aagccttgaa ctgtactaca
    61741 gaaaaattca taaatagatt tgactttata gaaaaaagtg caatgaactt gaataaaaaa
    61801 ttagaagaca tgacacttga agagatggat gagttttgga atcaggctaa aaaacaataa
    61861 aattgtggaa ggaaatttgt caaaaatcta gaacttttat cacaaagata gaagtctata
    61921 ttataaacat ttttaggagg taaatatcgt gaataaagct gaattagtat caaagatggc
    61981 agaaaaaagt ggattaacaa agaaggaagc agaagctgcg ttaaacgcat ttatgagttc
    62041 tgttcaagat gcactagtaa ataatgaaaa agttcaatta gttggatttg gaacatttga
    62101 gacaagagaa agagctgcta gacaaggaag aaatccaaga gatccagagc aagttataga
    62161 tataccagct tctaaagcac cagttttcaa agctggaaaa ggattaaagg atataataaa
    62221 tggataatta gaaatcttgt gaaaaagtgt ggataaattt gccacacttt ttctatagaa
    62281 ccaaggaagg tgataattat gagattagat aaatttttaa aagtatctag gattataaaa
    62341 agaagaactg ttgctaaaga tgcatgtgat aaagggattg tcactataaa tggaaaagtt
    62401 gctaaatctt cttcagaggt gaatattgga gatacattag aaatacaatt tggagagaag
    62461 aagatgaagt ttaagataaa tgaagtaaaa gagcatgtat taaaaaatga tgcaaaagaa
    62521 atgtacaaaa ttttagaata aaattaatag tcctctagta tattaatagt agtattattg
    62581 aaatatttac tggggggata ttatggaaca gaacataact ttgaaagata gatcaaagct
    62641 ggttatttcg ggagtcgagc acatatactc ttttaatgac aaaagagtag agctcaaaac
    62701 ttctgtaggc gagatggtaa ttgaaggcga aaacctagat atgagcaagc taagtataga
    62761 cgaaaatata attagcatag atggaactat aaattctatg gtatatgcaa agcctaaaaa
    62821 gccacaagag agtttcttga aaaaggtatt tagatagatg atacctttta ctcaagatgt
    62881 aagcattttt tatgctacta tttatggtgg aatactcata ggggtgttgt ttgattttta
    62941 tagagggcta agagggaatt ttaaattcat caattatttt gctataatct ttgatgtatt
    63001 gttttggttt ctagctacgg ttataatatt cgtaacaata aaccttacag aattttttga
    63061 tttaagatat tatcattttg tggctttgtt tattggtttt attttatact ataacaccat
    63121 aagtaaaatt gttttatcaa ttatcaataa aataattcgt tttgtgagaa attcttttaa
    63181 aaaagttaca cattacatag tcagtttttt aaataatttg tattacgtta taatctattc
    63241 actacaccta ctatttgata ttatatttta tataccaaac atatttatag caacaagaaa
    63301 aagtataaaa agaaaatcaa ataaaaaatt aaagaacaag aaaaaatcaa aacctaagaa
    63361 gaaaaaaaat aaaactaaaa aaagggtgta ggaaaaatga acttaagaaa aaaattttca
    63421 ggacagatta ttgttatcag tttattttta ggaatatcaa tttttagtat gatgacagga
    63481 tttgtatttg agtatacaaa agctaaggaa tataaaaaag aaatagcaag tctaaataaa
    63541 caattaaaaa agactgaaat acaaataaac agcttaaaga aagatgaaaa atcatatgaa
    63601 ggagacttag aggatatagc aagaaaaaga ttgaatatgg taaaaccaaa tgaaacagtt
    63661 tatgtagaca taaatcgttg attaaaactt gctaataagt ttacagatgt attgatttta
    63721 aaatttaaga tttaataaaa aagtcattca gttaaggatg gctttttgtt tttattgagg
    63781 agggaatttc ataatgaaag taggagctat agatattggg acaaactcaa tgagattatt
    63841 gatagcagat tatagagatg ggaattttat aaacagaaaa aaatttgtta atacaactag
    63901 aattggttat ggtgtggata gtaaaggata tataaaagaa gatgctatca aaagaaatat
    63961 aaaagcatta aaagaatttt ctgataagtg taatgatgaa aagtgtgaaa aaatttattg
    64021 tatggggaca tctgctctta gagacagtaa aaatggagat tactttgtta aattagctaa
    64081 agaagaaact ggaatagatg ttgatatagt tacgggaata gaagaatcaa atttgggctt
    64141 taagggtgtt ttagaaggaa tacaaggtga tgatactatt attgttatag atattggagg
    64201 aggttcaaca gaatttattg taggtgattc agaaggaatt aaatttaata aaagtgagaa
    64261 tgtaggtgct cttaggatga cagagaggtt tttgaagcac gatattattg aagatgaaga
    64321 gtttgaagct atgagttcat ttatatatga tgagattgac aaaacgttaa atcatataaa
    64381 agacagagga ttaaaaactc ttataggtat aggaggtacg attacatcat tatcagcaat
    64441 gaatcaagaa ttagagattt actctatgga aaaaatacat aatagtaaaa tacacaaaaa
    64501 agaaatagaa gaaattctac aaaaattaat aaagatgaca ttaagtgata aaaagaactt
    64561 aaaagggcta caagaaaaac gtgcggatat tataactgct ggtgtaaaaa tattgaatat
    64621 aataatggaa aaattagaaa tagaagaaat aaccgtaagt gaatacgata atttggaagg
    64681 cttaatatgt caaaagtcaa aaaaaatgta ttaatatttt ttgattttta aaaccgatta
    64741 tgacaaaaaa aattactcct ctttgttaca attcttaata taaacaaaag aggaggtttt
    64801 tttatgcaaa gaagtgtagc agttgtgaag aataagactg taaaaccaaa tgtcataaaa
    64861 ttaataagcg gatatattga ttttactacg gcggcattcg ctgtgatggg atttttactt
    64921 agtaggtcta ttatagtaga ctctgtagct cctttaggaa ttgccttttt tatatgtgca
    64981 tcaaagattg ataagtacaa gatacctgta ttcatatcaa cattattagg aatattgtta
    65041 tcatctaatg attttggaaa tgtagcaaaa tatgctgtgt gccttacaat atttatggct
    65101 atgaataaaa aattaaaatt atcagattca attgctaagg ttgcatttat aggtgcaata
    65161 atactacttc caatctccat gggacaagct ttcttttcaa ataggtatgt atatggtctt
    65221 cttatagcag gagtagaagc aataataatg tttatatcta tatatgtgtt ttcttatggg
    65281 ataggtttaa tggccaatgt caacaatagg atgtctgtac ggacagaaga agccatatca
    65341 ataagtttac tgctaacctt tagcataatg ggaataggag atatatctct gtttggaata
    65401 tcaataagga ccattttatc gaccgtattg atattgctga cctcaatagt aggtggagct
    65461 accatgggag catctagtgg tgtaattatt ggtgtagcat ccattttaaa taatatgaca
    65521 tcagctgttt atatggggat atattctttt tcaggattaa taagtggagc atttaataaa
    65581 ataaataaat atttctgtat actaggatat attctaagtt ggacaataat atacctatat
    65641 acttctggta ttacttctaa tatgatgcaa ttaagagata tactattagg ttgtttgata
    65701 gtactagtat tgccagagag attatttaat aagattgaaa aattaataaa aagtaatgtg
    65761 gcttcaaatg aaatcgtata cgattatatt atgagaagca aaaacctaac taatagcaga
    65821 ttgaacagta tttataaaac ttatgatgac cttgcagata catttgataa gataagagaa
    65881 aaagacaaag tgctagacca aagagacata gctaatgtaa tagatatgat tcataatgat
    65941 gaatgtaaaa gttgtagtat gagaagaatg tgctgggagt caagatttaa tcatacatac
    66001 actatgatat ataacattct tgaaaaaatt gaggagaaag gggagttaag tctaaacgat
    66061 atacctaaaa attttagaaa ggaatgtatg aaaccagagt caatagtgaa aatatcaaat
    66121 cactattaca aaatgtttgt attagattat gattggagtg tgaagttttc agaaagtaga
    66181 aagttaatag caaatcagat aagaagtatt tcaaaatcca ttaaatcctt atctcaagat
    66241 ttagaaggcg acataatgct agatatagaa aaggaaaaaa atatatatga acagttagaa
    66301 agatatgata taacagttga taaagtaagt tacttaacaa aaagtaacag tgagtttgaa
    66361 atatcaatag aaaagaaaac ttgccatgat ggatgtatgt gtgaagataa aattgtaaat
    66421 ataatttcag acctagtagg ggaaaatatg tctgttagaa aaataggatg tcactgttta
    66481 ggaggtaaat gcaaggcaac attcgttaaa tctcaaaaat ataaggctgt tacagaagtt
    66541 tcagcaatgt caagggatgg gcatatcctt tgtggagaca attacacata catggagata
    66601 aatgatggta aatacatgat ggctataagt gatggtatgg gaaagggtaa aaaagcatat
    66661 gaagaatctt ctgccactat tgatatactg gaaaaaatga tagatgcaaa aattaaagat
    66721 gagatagtca ttgatactat aaacaatatg ttgttgttaa aatcttcaga agagatgttt
    66781 tcaactctgg atttgggaat tttagattta aaacgaggtt gtttagaaac tataaagatg
    66841 ggagcgtgtt ctacttatat aaaaagagag gatggagaag ttgacttaat atcatcatca
    66901 tcattacctg tgggtatatt atcagatgta aaaattgata gaaaaaatgt aaaagttaag
    66961 gaaggagact atgttataat ggtttctgat ggaattgtag atgcaggaag aaataacaat
    67021 ttaggagaca attggttaat atacttctta aagaatatag agactacaaa tccaaaggaa
    67081 atttcaaatt taatattgga tagagcatta gagttacaag cgttacaaat cgaagatgac
    67141 atgactgttt tagttacgaa aatatgtaca aaataaggtt tacaagatta tgtgtataag
    67201 atgtggataa cttgtgtata atatgttaat agacttttga aaaaagtata gttattaaca
    67261 aaaataacat ataaatcaac aaaatttgtt gataagtatg gcagaaatgt taataaaatg
    67321 taaaaaaaga aaaaacaaaa gctattatca aaagagattc atgttgaaat aactaaattt
    67381 ctaagtaaag aaaacctgtg aatatgttga ttattatgtg gataactatt aattcgatat
    67441 ttatttatat agtatgaata attgaataat atatctaaaa aaagccatcc tattattaag
    67501 taaacttatt gaaaggatgg ctttttatga atagaaaatt gtgcaaagca ataatacctt
    67561 tatttatttc tattttttgc atgttaataa taagtataaa agctgaccaa ggatatcaaa
    67621 agaataatga cagagcaatt actgtcatga ataataatga tactgaagat gatgctgaaa
    67681 aaaaacaaga aatagactat gaaaaaacat gttggaattt gcaatctttg tttaaaagtg
    67741 atgaccaatg gaaaaaagaa cttaagagtt ttgataaaga tactaaagag ttgaaaaatt
    67801 acataggtaa agtaacaaaa tcgccaacac atttgtcttt tgccttgaac attaaagaaa
    67861 aacttgatat aagactaaat aaactttctg cctatgtaaa attaaaacaa gatactaata
    67921 aaaattcgta taagtactta gatatgaatg atagtatgag taaatcctat gggaattata
    67981 tggggatttg ttcagattta gagttagaaa tactaaaatt atcaaacaaa gattacaaga
    68041 aaattatttc aaataaaaat ataaatagga aatatggtgc atatttaaga gatataagaa
    68101 ggaataaagt acattatttg gatgataaat cagaagatat tctaagcaaa gtatcttcaa
    68161 taagtagtct tccaagtcaa gtgtatgaat tatttagaaa tatggataga aaaactaatt
    68221 taacaccagc tcaatatgcg agtgaattag agtcatcaga tagagaaaat agaaaaaaag
    68281 cttatcaaga tgaacttata acttacaatg ataatataaa tacaattgca ggtttaatta
    68341 ctggtcaagt taaaaagaat attttttatt ctcaggaaag aggttatgag tcttctttag
    68401 aaatgtacct ggaagctgat aatgtagatg aaaaagtata caataatttg ataaggactg
    68461 taaatagtaa tatggacagt ctacacaaat atatagattt aagaaaaaaa gtattaaaat
    68521 tagataaggt tcattcatat gatatgtttg tgcctatggt taaggctgtt gataataata
    68581 ttaattatga aaaggcacaa agtatagtat attcagcgtt gtcaccttta ggaaaagaat
    68641 acaatgatgt agtatataaa gctttcaatg aaaaatggat agatgtttat tcacatgaaa
    68701 ataaagtaag tggtggatac tgcttatctg tttatgaaaa tcatccatat atacttttaa
    68761 attataataa ttcattaggt tcagtttcga ctttatcaca tgagcttgga catgctgtat
    68821 atgaatactt aagttctaag aatcagaatt attttaattc aaatccgtct atatttactc
    68881 acgaagttgc atctacaacg aatgaagcat tactttacga gatgcttata aaagaagcta
    68941 aaggcaatga tgaaaaagct tattatataa cacagtattt agacttgata aaagatactc
    69001 tttatacaca aactatgtat gctgaatttg aaaaaactat acacgagtta gtagaaaaag
    69061 gaaaaagtgt aaacgcatta gtattaaatg atgtttgggg acaacttctt aaaaaatatt
    69121 atggacaaag ctatgaatta gaccaattat ctaaggtcgg atgggctaga atcccacatt
    69181 tttataatag tttctatgtc tataaatatg ctacaggttg tagtgcagga gtaagttttg
    69241 cccaagacat cctaaagaat ggttcagata actatatgag ctttcttaaa aagggaagtt
    69301 cagattatcc aataaacttg ttaaaatcaa gtggcataaa tttaactaat acaaaaccca
    69361 tagaaaatac aatcaaaaaa tttgatagtt tagtagcaga acttgaaaag ctattataaa
    69421 cattattgta atccacatgg taaatatatc gaattaatga taaaatatac taaaagttta
    69481 aagtcctatc aagataggac tttttattta ttaattagtg ggagatgatt ctatgcaagt
    69541 taaagttaaa aaggctgtaa taccagcagc aggtttagga actagatttt tgccagctac
    69601 aaaggcacaa ccaaaagaga tgttaccaat agttgataag ccaactctac aatacataat
    69661 agaagaagct gtggcctcag gaatagagga aatactaata ataacaggta gaaataaaaa
    69721 atctattgag gaccactttg ataaatctgt agaattggaa cttgatttag agaaaaaagg
    69781 taagaaagaa ttgttggagg tagtacaaaa catatctaac atgattaaca tacattatat
    69841 aagacaaaaa gagccaaaag ggttgggaga tgcaatttat tgtgcgaggc attttatagg
    69901 tgatgagccg tttgcagtaa tgttagggga tgatattgta gataatgatg taccttgtct
    69961 aaaacaacta acagatgcat atgaggaata tagaactaca atattgggtg ttcaaaaggt
    70021 aaatcaagaa gatactaata aatatgggat aatagaagca aaaaatatag aaggtagagt
    70081 ttacaaggta aaagacatgg tagaaaagcc agagtcagga aaagctcctt caaatattgc
    70141 tattttaggt agatatataa taactcctga aatctttgat attcttaagg atttaccacc
    70201 aggtaaaggt ggagaggtac aacttacaga tgctttaaaa atattatcta agaaagaagc
    70261 tatgtatgca tacaattttg aaggcaagag atatgatgtt ggggacaagc taggattttt
    70321 agaagctact gttgactttg ctctaaagaa agaagacctt aaggaagatt ttataaaata
    70381 tttgaaacat gtatgtagtg aatttgataa aaataacaat gtgttataca ataatacaga
    70441 tgaaaaaata gatttaattg aagtcaatga agctagagaa cttgaaattc aaaaataatt
    70501 aatgaaaggt gatgtatggt ggcaaaatca agtcagaaaa gaattaatag aataatagct
    70561 gttgggattg tgggaatcct cgtaataacg agtataacag ctgttttgag tatgattgca
    70621 actatgtaat aagataaatg acagcagttg atatacataa atataaaaac catatataac
    70681 ttgatgtagt caaaaactta caatatttta gttatatatg gtttttagaa tttataaatg
    70741 taaaaaaaaa tttttataat agttgcatat tttaaaggaa aatattatta taagatataa
    70801 atatagtgat aaatttataa aaacgtgcta agattggact tgaaatacag taatatagta
    70861 ggtaataatg ttttaaattt tatttattga ccataatata taaataatga tattattaga
    70921 cttaactgtt taaataaaac ttttcttttt catgccttca atttccaaaa taagtatgta
    70981 tataaatgaa tatatagatg aggaggattt ttttgatttt agatgataaa gtattagaag
    71041 gattaggtag taagacctta gttgagctac gagagatagc taaagaatta aaaataaaat
    71101 caattactac atataagaaa aatgagttaa ttgagattat aaattccaaa agtaaaaatg
    71161 aggaaaaagt aaacgaagag actaaagaaa aaaatgaaga tattaaagaa gacataaaga
    71221 atagaaaaga aaatatagag cgtgaaagta tagaaaaaga agttgaatat aagagcccta
    71281 ccaaatcata caacaagaaa gaaatagata cccaaaatat ttcccaaaac caagaacata
    71341 gaaatcaaag aaatgactat aataagacaa cgagtgtaaa tgatagtaat aagtcatcaa
    71401 atatatctaa tagaatggtc agaaataata ataaaaacta ttatatgcct aaacaagttg
    71461 atgaatctaa gatagtagat gaatttaaca catcaaaaga ggatgaagta gtaggtgtac
    71521 ttgaaattct gccagatgga tttggttttt taagaggttc aaattatttg tctactgaag
    71581 gagatgttta cgtttcacca tctcaaataa gaagatttaa tatgaaaaca ggagacaaga
    71641 ttaagggaat aactagacat ccaaaaagtg gagagaaatt tagagcactt ttatatgttc
    71701 aaaaaataaa tgatgaaaac cctgacacag ctatacaaag aaatgcgttt gagacattaa
    71761 caccaatatt tccagaagaa cgacttacac tggaaactaa tagaaatgaa atagctacaa
    71821 gaattataga tttaatttca cctataggaa aaggacaaag aggattgata gtagcacctc
    71881 caaaagctgg gaaaacagtt cttttaaaaa gcgttgcaaa tagcatagct aaaaatcatc
    71941 caaatgttga acttatagta ctccttatag atgaaagacc agaggaagtt actgatatga
    72001 aagaatctat tgaaggagac gttatatatt ctacttttga ccaagtatca agtcatcatg
    72061 tcaaggttgc agagatggtg ttaaatagag ctcaaagatt agttgaacat ggtaaagatg
    72121 tagtaatttt actagatagt ataactagat tggcaagggc atataatctt acaatatctc
    72181 ctacaggtag aactttatct ggaggtattg accctggtgc acttcatgga cctaaaaagt
    72241 tctttggtgc agcaaggaat attagacagg gtggttcttt gacaatatta ggaacagcat
    72301 tggttgaaac tggttctagg atggatgatg taatatttga ggaatttaaa ggtacaggaa
    72361 atatggagct acatttagat agaaaattag cagaaaaaag aatattccct gcaatagata
    72421 tttacaagtc tggtacaaga agagatgatt tattgcttga tgatgaagaa aagactgctc
    72481 tttggagatt aagaagagaa atgagtaata attcagtaat ggaaattaca gataaagtca
    72541 ttgaacttat aaagagaact aaggacaata aagagtttgt aaaatcaata aagaatttgt
    72601 aaaatcaata atacaaatgc aaatatggat tatataaact aatagaaaaa tcacttgaat
    72661 ggcataataa gttgtgctat aataatgtag ttgataatgt aaaaagaaag ttaatataat
    72721 taattttttt aaataaagag aagaggtgac tataatgcaa aaggaaatac aaccaaaata
    72781 caatccagtt gaagtgcgtt gtgcttgtgg gaacacattt gttgctggtt caactaagga
    72841 cgaaataaaa gttgaaatat gttcagagtg ccatccattc tatacaggaa aacaaaagaa
    72901 catagaaaaa ggtggaagaa tcgacaaatt caagaaaaga ttcaaaatgg actaattttt
    72961 ataaaagtgg agttggagga agccaactcc tattttatta caaaaaaaca ttcatattct
    73021 actttaaatt gtctttttgt attataataa taaatatgta aaattgagta ttctaaatac
    73081 aataaaatga ggtgagtgga tggcaaaaca agctgtaggt gggcaagctg ttattgaagg
    73141 tgtaatgatg cagtcaaaag acaaaagagc ggtagctgtt agaaaaagtg acggagaaat
    73201 agcactaaaa gaagatagaa taaaaagttg ggtaagagat aagaatatag ataagatacc
    73261 ttttgttaga ggttcttttg ttatgataga tacaatgatt caaggcataa aaagcttaaa
    73321 cttttcttca gagtttttta tggaagaagc tgaagaggat aaatttgatt tatttataaa
    73381 gaaaattttt aaagataagg caaatgatat aattataata ttttcattgg tcatagcaat
    73441 gttactttct gctggattat tcatttttat acctacattg gtaggaggag ctttttctaa
    73501 agtaatgcct aatgatttta tgcttaattt aatagaagga atcataagga tagctatatt
    73561 atttgcatat atagtattga tttcacgaag taaggatata gaaagagtct ttcaatatca
    73621 tggagcagaa cataaatcta tatattgcta tgaaaatgat ttagaactta cagtagagaa
    73681 tgctagaaaa tttaaaagat tacatcctag atgtggtaca aatttcctat ttatagttat
    73741 ggctgtatca ataatattgt ttgcattttt tggttggcca aatccaattc ttagaatatt
    73801 tatgagaatt atatgtgtgc ctatagtagc aggtttatct tatgaggtaa ttagagttct
    73861 tgggaaatat gataatggat ttactaaaat tattgcatat ccaggaatga tgttgcaata
    73921 ttttacaaca aaagagcctg atgatgagca attagaagtt gctttggaag cattgaaagc
    73981 agtagtagat tagagaaaag gatatttgtt atgacgataa aagatataat tattaaatat
    74041 tcagataaac taaaagatat aagtgatact ccaagattag atacagagtt attgttacaa
    74101 aaaacattgg gtgttgatag attatatatt cacttaaatc tgaataaaga gctaactgaa
    74161 gaacaaaaaa caaaatttat gggttttgca gaagaaagat taaatggaag accaatagca
    74221 tatatagttg aaaataggga gtttatggga ttagattttt ttgttaagga aggcgtattg
    74281 attccaagac cagatacaga aactttagta gaagaaataa ttgaaatatg tagagaaaaa
    74341 aaagatgtaa gtatattaga tataggaact ggttctggag ctattacaat aagtttagca
    74401 aagtacattg aaaattctaa aatcatgtct tttgatatat cagagacagc attagaaata
    74461 gctaaaaaaa atgctattat aaatgaagtt ggtgaaaaaa taaagtatat taattctgat
    74521 ttatttacag caataagtga tagtaacata aaattcgata tcatagtttc aaatccacca
    74581 tatattaaaa aacaagatat agaaactctg cacaaacaag ttaaagatta tgaaccatat
    74641 aatgctcttg aaggtggaga agatggatta gacttttata gaagaataac agaacaaggt
    74701 aagaaatatt taaataagtg tggaatatta gcatatgaag ttggtcataa tcaagctgaa
    74761 gatgtaataa atattatgaa aagtaatgga tataaaaaaa tatacacaaa gaaggatatt
    74821 caaggcattg atagagttgt tataggttac aatatatgat ataatcaaca attgatatgt
    74881 tacatttaat cgaggtgaaa gtatgttaaa aaaattagag gtattggaag atacatataa
    74941 agacttaagt gaaaaaatag gtgatccgga tgtaataaat gatcaaaaag tttggcagaa
    75001 atatataaaa gaacatgctg acttggaacc tattgttatg aaatacagag agtataaaag
    75061 cgttttagat agtataaaag aatcaaaaga aattttacaa gaagagtcag atgaagaatt
    75121 aagagaatta gctaaaatgg agttagctga gatggaagaa aaagtagcac ctctggagga
    75181 agaaataaaa atactattat tgcctaaaga ccctaatgat gataagaacg ttatagttga
    75241 aattagaggt ggagctggag gagatgaagc agctctattt gctggtgatt tatttagaat
    75301 gtactcaagg tatgctgaaa gaagaagatg gaaaatagaa ttattgagtg caagtgatac
    75361 tggagttggt ggatataaag aagtatcttt tatgataaaa ggtaaaggtg cttattcaag
    75421 attaaagtat gaatctggag ttcatagagt tcaaagaata ccatcaactg aatctggtgg
    75481 aagaatacac acatcaactt ctactgttgc agtattacca gaagttgaag atgtagaggt
    75541 tgaaataaat ccaaatgatt taagaataga tgttttccgt tcttcaggta atggaggaca
    75601 aagtgtcaat actactgact ctgctgttag ggtaacacat atacctacag gtgaagtagt
    75661 atcttgtcaa gatggaaaat cacaattaaa aaataaagag caagctttaa aaatattaaa
    75721 agctagactt tacgataaag cattggcaga acaacacaaa gatatagcag cagaaagaaa
    75781 aagccaagtc ggaacaggag acaggtcaga aagaataaga acatataatt tccctcaagg
    75841 tagaataagt gaccatagaa taaatttaac tttatataag ttagatgcat tcttagatgg
    75901 agatatagat gaaatgatag atgctttaat tactgttgac caaacagaaa aaatgactgc
    75961 aatataagta atgttttaaa gaagctgttt cttcctagaa attattttct attttgaaac
    76021 agctattttg cattttaggt catgttatca tattctattg tatataattg ttatatacaa
    76081 gtataggagg gttatatgat actaaaagta acaataattg ggcttttggc aggtgttata
    76141 gggacaggat taggaggagt aatatcagcc atttttaaaa gagaggtcga taaatatcta
    76201 agttttttta tgggattatc tggtggaata atgttggctg tagtggtgtt tgatttaatg
    76261 aaagaatcca tggacaaaat ggggataatc aatacagtta tatttacgtt tgttggagct
    76321 ctcataacta tgtacataaa aacaaaatta gatgtgtctg gaaatatggc atctggatat
    76381 cttatattca taagtatatt actacataat ttaccagaag gactagccat tggttcttct
    76441 tttatgtcaa ctgaaagttt aggtattaca cttgctatag ttataggtct acataatata
    76501 ccagaagggt tagctatggc attaggctta gtttgcaata aaatgaaact aagtaaagtt
    76561 attttgttta ctgtaatagc tggattacct atgggtctag gaagtttttt aggtgtttat
    76621 tttggaggag tatttacttc tcttatagga gtatttcttg ctacagcagg aggtactatg
    76681 atgtatgttg tattggaaga aatttttcca cattcaaaaa gtgtgtattg tataattggt
    76741 ttcttgttag gtacaatgat tgtaaattat atataaaatt aaaatgaaag caggcataca
    76801 aaagatgaaa actattataa gtaatattga tataaataat attgattatg aggaaataaa
    76861 aatacaagca aaacttctta gagaaggtaa aacagttatt tttccaactg agacagtata
    76921 tgggttgggt gcaaatgcat tagatgaaaa tgcagtaaag aagatttatg aagctaaagg
    76981 aagacctagt gacaatccat tgatagtcca tatatatgaa aaagaagaag tatatgactt
    77041 agccaaagat ataagtgata aagctaaact tgttatagaa aagctttggc caggacctat
    77101 aacaattata ttaaataaaa aagatataat tccatacaaa actagtggag ggcttgatac
    77161 agttgcaata aggatgcctt ctaatgttat tgccagggca ataataaaag aagctggtat
    77221 acctattgca gctccttctg caaatatctc aggtcgccca tctccaacaa aagcaaaaca
    77281 tgtatatgaa gagatgaatg gaagagtgga tggaatagtt ttaggaggag acagtaattt
    77341 tggattggag tcaactgtat tggatttaac agaagagact cctatgatac tgagaccagg
    77401 aagtataact aaagaagttt tagaggaact tcttggagaa gtaaagcttg acccatcatt
    77461 aagtaaaaaa gaagataatc aaaaagctaa agctcctgga atgaaatata aacattattc
    77521 tccaaatgca gatgtatata taatatctgg aaaaagagaa aatgtagcaa caaagattaa
    77581 tgatatgata aaatctaata gagacaaagg tctaaagaca ggtgtaatgt gtattagtag
    77641 aaaccgagag ttttatgatg gagaagtaat cgatttagga aactctcttg aagaagttgc
    77701 aagtaatctg tttgatgctc ttatagaaat ggacaaaaag ggtgttgatg tcatatattc
    77761 agaagagttc cctaagactg gagttggaca agcaattatg aatagactac tgaagtcagc
    77821 aggatacaaa gtgataaagg cttaagaggg agttgtaaaa tatgaatata cttattgtgt
    77881 gtacaggtaa tacttgtaga agtcctatgg ctgaggctat actaaggaaa gcaattaaag
    77941 aatctggaag aagtattgaa gaatattcta tatcatcagc aggtatatct acagcaaatg
    78001 gaatgggtgc ttctgaaaat tctatagaag tactaaaaga aatcggtata gacttatcaa
    78061 atcatagaag taaagtaatt actaaaaaat taattgatga atcagatata atactaacaa
    78121 tgactaaatc tcataaagaa attttagtac aagcagttcc aaaatgcaaa gaaaaagtgt
    78181 atacttttaa agggtttgca aataaaaatg aggaagatat atctgaccca tttggtggaa
    78241 atttggatat ttatagaagc actatgagag aaataatgta ttctgtcaac gaaatagtaa
    78301 aaaaaatatg aaacggagga taaacaaatg aagataggat taggctgtga ccatggagga
    78361 tataatttaa aaaaagaaat aataagctat ttagaaagta aaggcataga atgtgtagat
    78421 tatggaacta ataatgctac agattctgtt gattatcctg tttatggaga aatagttgca
    78481 aattcagtta taaataaaga agtagattat ggaatattat gttgtggtac agggatagga
    78541 atatctttag ctgctaataa ggttcctggg ataagatgtg cagttgtatc agatgtattt
    78601 tctgcaaaaa tgtcaaaagc acataatgat gcaaatatgt tatcattagg cgaaagagtt
    78661 ttaggaaaag gtctagcact tgagatagtt gaagcatgga ttaatacgga ttttgaaggt
    78721 gatagacatg ctagaagagt aaacatgatt aaatcaatag aagaaaaaca taataaataa
    78781 taaaaattac agttttatca ggaggaatta tcaaaatgag caaagtggta gagacaaacc
    78841 atccattaat acaacataaa ttaactttaa tgagagataa aaatactggt tctaaagact
    78901 ttagagagct tttaactgaa attgcaatgt taatggggta tgaaataaca aaagatatac
    78961 cattaaaaga tgtagaaata gaaactccta ttcaaaaaac atcatctaaa gtagttgcag
    79021 gtaaaaaatt agcaataatt ccaattttaa gagcaggtct tggaatggta gatggattag
    79081 tgagcttaat gccagcagca aaagttggac atgtaggact atatagagac ccagaaacat
    79141 taaaaccagt tgaatattat tgtaagcttc ctcaagacat aggagaaaga gatataatag
    79201 tagttgaccc tatgcttgca acagggggtt ctgctgtagc agctatagat ttacttaagt
    79261 ctaaaggagc taaaagtata aaattagcta atttagtagc tgcaccagaa ggtatagcag
    79321 aagtgcaaaa atatcatgat gatgttgata tatatgttgc atcagttgac gaaagattaa
    79381 atgaacatgg atatataatt cctggtcttg gtgatgctgg agatagattg tttggaacta
    79441 aataaaaata aataactata aatatttatt agggactttc ttatatagtg tatataattt
    79501 tatgtaagaa ggttcttttt tatatatttt ttctcaaaat aggaattatt gaattgataa
    79561 atttctcgaa ttgagaataa aaagttttta ccaattaaaa aagattgctt attttacaag
    79621 gttttataga atagtagtct attttaatgt ggcatgttag ttgcttacga ttaagattat
    79681 aggtattaag tgtattagtt aaattgagaa ttgaatatta ataatattat tgggagtggt
    79741 aatacatgaa gaattgtata gtagtaagag gggctggaga tttagcaaca gcagttattc
    79801 ataaattaca caacagtggt tttaaagtag ttgcgttaga aatagaagag ccattagcta
    79861 taagaaggca aatttctttt tgtgaggctg tttatgaaaa aaaagtaact gttgaaggtg
    79921 tagaatgtat attttgcaat actgaaaaag aaatagaaga agttttatct caacataaag
    79981 tagccttgat tatagaccca aaaggagaaa aattatctaa attaaatcca atcgttttgg
    80041 ttgatggaat actcgctaag aaaaacttag gtacaactaa agatatggca ccacttacaa
    80101 tagctttagg tcctggattt tgtgcaggag aagatgtaga tgttgttata gagacaatga
    80161 gaggtcataa tctaggaaga ataataacta atggtagagc agaaaaaaat acaggagtac
    80221 caggagcaat aaaaggtttt tctaaagaga gagttatgca ttcacagtat gatggcataa
    80281 tggaaaatgt atgtaagata ggtgatatag ttgaaaaggg agacctactt tgctatatta
    80341 ataattatga aaaaaaacat gaagttagag caacaatatc tggagtcctt agaggtttat
    80401 taaaggatgg agcaaaggta aacaaaaatt taaaaatact agacatagac cctagaaaag
    80461 aagaagttaa gaatgctttt acaatatcag ataaagctag atgtatagct ggaggggttt
    80521 tagaagccat actaaacaat gggatttatc cccaaaatta atttatatta gataaagcta
    80581 ttaatatttt attaagatga tataataaat ttaatagatt aaagagagca tatgacgggt
    80641 atttaaaatt taaatggtaa aaatgctctt tttttattgg acattcaatt aacatgtgta
    80701 tataatataa aaataagtat ttatttaggt tgaaatctaa taaatatata ataccagtac
    80761 ataagcaaag aggagaaaag taaattatga gaccatcatg ggatgaatac ttcatggaaa
    80821 ttgcagaagt agtgaaaaag cgttcaactt gtattagaag acaagtagga gctgtaattg
    80881 taagagacaa acaaatatta acaactggat ataatggttc tccgagaaat cttgaacact
    80941 gtgaaaatat tggatgtaag aggcaggaat taaatattcc ctcaggagaa aggcatgaat
    81001 tatgcagagc attacatgct gaacaaaatg cgataataca agctgctcat aatggtatta
    81061 gtgtagatgg ggcaactttg tatgttacta caagaccttg tgttttgtgt gcaaaaatgt
    81121 gcataaatgc aggtatattg aaaattgttt atgaaggtga ttatccagat gatatgtcta
    81181 cagaattatt aaaagaagca ggaatagaac tcataaaatt ttagtggata aaatttatat
    81241 gacaataaat gtcaaattat gacactgaaa tacaatggta tcaaatgatt aaaagaaagt
    81301 atttgaaaaa tcatcagatt agtagtaaga tatattttat aattaaataa atttatgaga
    81361 tagattaatt acatgttatt tattttataa taatgaggga tactatttat gagtaaaaga
    81421 aatacacata cagaaattgc aaagttgttt agtttaataa gccaaatagg attgatgatt
    81481 gtaatatcaa tattaggatg tactttctta ggaaaatttt tagatagtaa attgaatact
    81541 actccagttc tgacaataat atttttattg ttgggtgttg gaggtgcttt tagtggtgtt
    81601 tataaaactc taatagtata tacacgaagg aagtgatata ttggatataa aattacttaa
    81661 ccaggtaagg tcagtaacaa aaggtatagt aatatttgat gtagttttaa ttgtgctaat
    81721 gttgataaca tcaacattaa gtaaaccagt attattagga cttatatttg gctctataat
    81781 agctatgtta aattttagat tattggcgat atcactggaa aaatcagtga catttcctgc
    81841 tgggaaggca caagcttatg catcagcaca atatgctgtt agattattta taatggcagt
    81901 tgttctaatt gcatcagcta cagtaccaca tttaaatata atcggaacag cattgggatt
    81961 attgagcaca aagtttgtca tattatcaaa aaactttata aataaattaa agaggaagga
    82021 ggcataagta tgaatcaagc gcattattat ctcataggaa actttaagat tagtgaaaca
    82081 atactagtaa gttggggaat aatggccttt ttagcgatag catcttattt tttgactaga
    82141 aacttaaaaa aagttccaac tagtaaagtt caaatattct tagaatttgc agtaggtggt
    82201 cttgctaaaa tggttacaga tgttatgggt aaaaatacag taaaaagaat gccatatatg
    82261 ataccataca taggaagctt gtttttattt ttcgcatgtt caaatttaag cccattactt
    82321 ggattaaggt caccaacaac cgacttagat acaactgctg catgggcaat gattacattt
    82381 tttatgattt attttgcagg agttaaattt aaggggttat catactttaa agaattggct
    82441 caaccacttg ccataatgtt accaatcaat attataggtg agttagctag accaatatca
    82501 ttaagtttcc gtccatttgg gaatatttta ggtggaacaa taataatggg tctgttttat
    82561 caacttttag gttttatttc tagtttaata ccaggattat caataccaat tggagaaata
    82621 ttaataccag tcccattaca cttgtacttt gacttatttg caggattgtt acaagcatat
    82681 atatttataa tgttaacaat ggtatttata tcaagtgcag gagagttaga ttaagaaatc
    82741 aacttatatt aagataaaaa taaaataatt taaaaaatta aagttttaag gaggaaataa
    82801 ttatggaaac agcaatagta gcggcagcat cagcaatagg agcagggata gcagttgcaa
    82861 caggtatagg tgcaggtata ggtcaaggta tagcagcagc taaagctgca gaagcagtag
    82921 gaaaccagcc agaagctaaa ggtgatataa catcaacttt attactagga gttgctatag
    82981 cagagtcttc agcaatatat ggtttggtta tatcaataat acttctattc gtaaatccat
    83041 tctttaaata cttaggaatg tagatttagt ccttaaatat tttgagatag gtttataggt
    83101 aaatcatctc aaacttataa tcatgagatg aactttatca tctcgtgatt ataagtagtt
    83161 atgatttatg gaaaggagga taattatgga aaaagcacta gtaggtataa cgtgggaatt
    83221 tgtgttccaa attgttaata cttttataat atttttactt ctgagaaagc ttttattcaa
    83281 gccagtatta aatataattg aatcaagaga aaatgatatt aaaagtgatt tggtagaagg
    83341 cgaaaaagca aaaaatgaag gtttagctct gaaaaaagaa tatgagtcaa aaatcaattt
    83401 tgccaaagat gaaggtcaag aaatcataaa acaagcaact ataagagcag agcagaaatc
    83461 agatgatata gtaaatactg caaagaaaga tgctcttgat ataaaagaaa aagcaaacaa
    83521 agatatagaa caagaaagac aaaaggttat aaatgaaatc aaaaatgata tatctaacat
    83581 agcactactt gcagcatcta aagttattga aaaagattta gataaatcta aacatgaaga
    83641 attgatagaa aactttataa aagaggtagg ggaagctaaa tgataaatgt aatagcaaat
    83701 agatatgccg aagccctatt tcaattagga gaagaagaaa attcaactga tgtcttattc
    83761 aaagaactag aaaaagtagt agatatgatg actaaagtta gtaaagattt ctacaaagtt
    83821 ttaaaatcac cattagtttc aaaatcagaa aagaaaaact tggtagaaat tatttttagt
    83881 aaagaagtaa gttctaacat aaaaaacttt ttaaaagttt tagtagataa agatagaata
    83941 tcatatttag aagatatcga attagcttat aaagaattat taaataagaa aaacaatgta
    84001 attgatggag tagcaataag tgctattcca atgagtgaaa cagatattaa agaacttgaa
    84061 gtcaaacttt caaataaata caacaaaaat gttactatag aaaatgttgt tgacaagact
    84121 atactagggg gagttctagt tagaattgga aatgaacaaa tagatggcac tgtgaaaact
    84181 cgtcttgata agatgaaaga aaagctatct gaagtaatat cttaagaatg gcggtgaacc
    84241 catgaactta aaacctgaag aaataagttc tataattaaa cagcaaataa aaaattatga
    84301 gaataaagtt gagttgacag atacaggtag tgttttaaca gttggggatg gtatagcgag
    84361 tgtatatgga ttagaaaaag ctatgtctgg tgagttgcta gagttcccag gtgaaatata
    84421 tggtatggca cttaacttgg aagaagaagt agttggagct gttatccttg gagatgacag
    84481 tgaaataaaa gaaggcgata tagttaagag aactggaaga atagttgaag ttccagttgg
    84541 agaagcttta ataggtagag ttgtaaactc actaggtcag ccaatagatg gaaaaggacc
    84601 tatagcgtac acaaaaacta gaccagttga atctgaggca cctggtataa tagatagaag
    84661 gtctgtttat gaaccattac aaacaggaat caaatcaata gattcaatga taccaattgg
    84721 tagaggtcaa agagagttaa taataggtga cagacaaact ggtaaaacat ctatagttat
    84781 tgacactata ctaaatcaaa aaggaaaaga tgttatctgt atatatgtag caattggaca
    84841 aaaacgttct actatagctc aattagttag ctctttagaa aaaggtggag ctttggatta
    84901 tacaatagta gtttcagcaa cagcatctga atcagcacca cttcaatata tagcaccata
    84961 tgctggagca gctatgggtg aagaatttat gtacaatggg aaacatgtac ttatagtgta
    85021 tgatgattta agtaaacaag ctgttgcata tagagaaatg tcattgttac ttagaagacc
    85081 accaggacgt gaggcatatc ctggagatgt attctattta cattcaagat tacttgaaag
    85141 agcagctaag ttatctgatg aattgggtgg aggttcaatg actgccttgc ctataataga
    85201 aactcaagct ggtgacgttt ctgcatatat accaacaaat gttatatcta taacagatgg
    85261 tcaaatatac ttacaaccag agttattcta ttcaggtgta agaccagcag ttgaccctgg
    85321 tatatcagta tcaagggttg gtggttctgc gcaaattaaa gcaatgaaaa aagttgcagg
    85381 aacattaaaa cttgcatatt cacaatatag agaacttgca gcattctcac agtttggttc
    85441 tgacttagat gaagatacta aaaagagact tgctcaaggt gaaagaatcg ttgagatatt
    85501 aaaacaaggt gagcatcaac caataaaagt tgaaaatcaa gttatgataa tatatgctgt
    85561 tataaataat catttagagg atattccgat agataatata gctagatttg aatcagaatt
    85621 gtatgcattt gtagataata attatccgga aatatcaaga aaaatattag gtggagaaga
    85681 cttcactcat gatttaacag atgcaataaa tgaatttaaa gaaaagtttg ttgttgaagt
    85741 ataatccttt ttaaaaaagg aggtaaatta attggcagga gctggaatca aagcaatcaa
    85801 aacacgtatg ggaactgttg aaagtacaaa gcaaataact aaagcaatgg aattagttgc
    85861 atcttctaag cttagaaaag caaaagaaaa ggctgaagat tcaagagaat attttaatac
    85921 tttatacaat acagtaaaag atattgccca aacaacacgt ggaattagaa atgaattttt
    85981 aaaagaaaga gaagtaaaaa ataaatgcta tgtagtagta gctggagaca gaggacttgc
    86041 aggtggatac aattctaatg tattaaagga atctgtctct catatgaaag gtaaaaagga
    86101 aacagtgatt actgttggaa agaaagcaca tgaatttttt tctaaaagaa actacaatat
    86161 agcaaaagat ataaatagtg ttgaagcttg tggatatgaa gatgcgacag aaatttcaca
    86221 aactattatt gacctgtata aaaaaggtga agtagatgaa gtttatattg cttatacaaa
    86281 atttaaatct gctttatcac aagaagttga aattttgaaa ttgcttccat tagtctttga
    86341 aaaagataca gacgaacaag gaatggcagt aaaagaagaa aaacaaaata gatcaaaagt
    86401 acaatatata ccatcagcag aagcagtttt aagttatatt gtacctcagt ttatatcagg
    86461 tacagtatat ggtggaattt tggaatcttt tgcatcagag caaggtgcta gaagaactgc
    86521 aatggaatca gccacagaca atgcgaatga aatgctatct aatttagagt taagttataa
    86581 cagagcaaga cagtcagctg taactcaaga aataacagag atagttggtg gagttgaagc
    86641 tctaaaatag ggaggttagg aaatggcaaa tgtaggtaaa gtagtacaga ttgtaggagc
    86701 tgtactagat gttaagtttg atagtgaaca aagcctacct aacttattaa atgcgttagt
    86761 aataaagtta ggagataaag aaatagttgc agaagttgct caacatatag gtgatgatac
    86821 tgtaagatgt atagctatga gtgcaacaga cggacttgta agaggtatgg aagttgtaga
    86881 tacaggaggg ccaataagtg ttcctgttgg agatgaaact ttaggtagaa tattcaatgt
    86941 tcttggaaaa ccagttgatg ggaaaccagc tccaaaatct gcacctaaat tacctataca
    87001 tagaccagca ccggcgtatg atgaactaga aactacagca gagatactag aaactggtat
    87061 taaggtagtt gacttactag ctccatatct aaaaggtgga aaaataggtc tatttggagg
    87121 agcaggagta ggaaagacag ttctaataca agagcttata aacaatattg ctaagcaaca
    87181 tggtggtatt tctgtatttt caggagtagg agaaagaaca agagaaggta acgaccttta
    87241 tggcgaaatg agtgagtctg gagttataaa taaaacagct ctagtatttg gtcaaatgaa
    87301 tgaaccacct ggagcgagaa tgagagttgc tttaactgga cttacaatgg cagaacattt
    87361 tagagatgag caaggacaag acgttttact tttcgttgat aatatattcc gtttcacaca
    87421 agctggttca gaagtttcag cacttctagg acgtatgcca tcagctgttg gttatcagcc
    87481 aacattagct actgaaatgg gtgcacttca agagagaata acatcaacta agaaaggttc
    87541 aataacatct gttcaagcag tatatgtacc agcagatgac ttaactgacc cagcaccagc
    87601 tacaacattc tctcacttag atgcaaaaac agttttatca agacaaatat catcactggg
    87661 tatatatcca gcagttgacc ctcttgaatc tacatcaagg attcttgacc ctagtatagt
    87721 aggaaaagaa cattatgaag tagctagagg tgtacaatca atacttcaaa gatataaaga
    87781 acttcaagat ataattgcta tacttggtat ggatgaatta tctgatgaag ataagttaat
    87841 agttgcacgt gctagaaaga tacaaagatt cttatctcaa tcattcacag ttgctgagca
    87901 atttacagga aatccaggtc aatatgttcc tgttaaagag actgtaagag gatttaaaga
    87961 aattcttgaa gggaaacatg atgacttacc agagtcagca ttcttatttg taggaacaat
    88021 agaagacgct gttagaaaag cgaagggaag tatgtagtca tggccagtga attttcattg
    88081 aaagttgtaa ctccaagtaa tactttttat gatggagatg ttgaaatgat agtagttaga
    88141 actacaaaag gagacaaagg ggtgttgaaa ggtcatatac catttgtttc aggtatacct
    88201 gtaggaactt taaagataaa aaaagaggga agttttaaag aggcgaaaat agcagaagga
    88261 ttcattaatg taactcaaga taaaactgtc atattgacag aatctgctga gtggatttaa
    88321 taaattaatt tagagtataa aaagtcattt tgaccaaaaa aacttttggg taaaatggct
    88381 tttttgtgtt ttggtgttaa taaaataacg ataaaattgt aagtttttag tgataataaa
    88441 ttcacaaaat gtttaaattt actgccaaat tatgttgtaa ttaataagat aaagaagatt
    88501 tagatagctt ttatgtaagt gaaaaagtct tcatataaga aatacatata aaatttttat
    88561 aaaatttaaa aaatttgctt agttgtgtag tataataaat aatataatag atttgtatat
    88621 aatattcatt acaaataatt ttaaaagtta cagttttatt ataatgatta ttaaattaag
    88681 ataagtaaca taaactaaat aataatgtgt attatcgata gtaaatttat aaattcatta
    88741 ttaattattg aaagaagtat tataaatttt ctggaggaac aatgaatata tttgatattg
    88801 gcgtagatat aattgaaatt gatagaataa gaaaggctgt agacaaaaat aataggtttt
    88861 tggagaagat atttacagat agagaaatag agtattttaa ttctaagaat tttaaagcag
    88921 aaagtatagc aggtaatttc gcagccaagg aagctataag taaatctata ggtacaggaa
    88981 ttagagtgtt taattttaaa gatatagaag ttctcagaga tgaaatggga aaaccaatag
    89041 taaaaactta taataatcta gcaaaaatgt gcatagatta taatgtttta gaaataaaag
    89101 tttcaatatc acattcaaaa gattatgcta tagcaaatgc gataaccata attaaggact
    89161 aaggagtgat tacattatga tggataaaac agcaaaagaa ataatgacaa cagatgtaat
    89221 agtagcaaaa caagatgata gtatagcaga tgtagctaat atgctaattg cagaaaaaat
    89281 aggaggtctg ccagttgtag attcagagaa tagagttgtt ggaataattt ctgaaacaga
    89341 tattcttaaa aaagaaaaat atatagaagc acctctctat ataaatctat tacaaggatt
    89401 gattttctta gatgacttaa aaaaagttga aaaagatata aaacaagtag ctgcttataa
    89461 ggttggagaa cttatgtcta aagatataat aaaggttcat gaagatgata agtttgatga
    89521 tgtagctaat gtaatgatta aaaaatcaat aaacagagtt cctgtagtag atgatgacaa
    89581 caaacttaaa ggtataattt gtagatatga tataataaaa gctttataca atgaataaac
    89641 tgaagtagga ggtgtgatgt gagaaaaaag tggaccatag tatgtattat gttcttggca
    89701 ctggttataa ttgtaatagg atgccaaaaa cgacagtcca caaaagaaga agtatacaaa
    89761 gattttcaaa agcagatttc tgatatgaat tattacagtt gtaaagctga agtagaagta
    89821 gttggaaata aaagtccaca caattatgtt ttaattcata cttataaaaa aaccgataac
    89881 tataaattag aagtaatttc cccaaaacat ttaaaaggga aaagtataga atatcaaggt
    89941 gataaaatat tagttaaaaa tccaaagatt agtgatgtag tggagttacc taatacagga
    90001 aaaaataatc aatatctttt tgtaggagac tttataaaaa actatcttca aaatgaggag
    90061 atgaaagtaa agttatccaa aggacatttg gttttagaga cttttattcc tggggataat
    90121 aaatatttta acaagcaagt attatatgta aatgctgaca caaaaaatcc tgaaaaaatg
    90181 gaagtgttag ataaagaggg agtgccaaga tttacagtaa aatacaaaga ttttgaatac
    90241 agaaactaag ggggactaaa gacatgcaaa aaataacagt gcctacatgg gcagagataa
    90301 atctagataa cttaagattt aacttaaata atattaaaaa tttattagaa gaagatatta
    90361 agatttgtgg agtaataaaa gctgatgcat atggacatgg tgcagtagaa gttgcaaaat
    90421 tgctagaaaa agaaaaagta gattacttag cagtagcaag aactgctgaa ggaattgaac
    90481 ttagacaaaa tggcataaca cttcctattt tgaacttggg atatactcca gacgaagctt
    90541 ttgaagattc tataaaaaat aaaataacta tgacagttta ttctttagaa acagcacaaa
    90601 agataaatga aattgcaaaa tctttaggag aaaaagcctg tgttcatgtt aaaatagact
    90661 cagggatgac tagaataggt ttccaaccta atgaggagtc agtacaggaa ataatagaat
    90721 taaataaatt agaatatata gatttagaag gtatgtttac tcattttgct acagctgatg
    90781 aagtaagtaa agagtacact tataaacaag ctaataatta taaatttatg tctgataaat
    90841 tagatgaggc tggtgtaaaa atagctataa aacatgtatc aaacagtgca gctattatgg
    90901 attgccctga tttaagatta aatatggtaa gagcaggaat aatattatat ggtcattatc
    90961 catctgatga tgtatttaaa gatagattag aattaagacc agccatgaaa ttaaaatcaa
    91021 aaatcggaca tataaaacaa gttgaaccag gtgtaggaat aagttatgga ctaaaataca
    91081 caactacagg taaagaaaca atagctacag ttccaatagg atacgcagat ggatttacta
    91141 gaatccaaaa aaatccaaag gttcttatta agggagaagt gtttgatgta gttggtagaa
    91201 tatgtatgga tcaaataatg gttagaattg acaaagatat agacataaaa gttggagatg
    91261 aggttatact atttggagaa ggcgaagtta cagctgagcg tatagctaaa gacttaggaa
    91321 ctataaacta tgaagtgtta tgtatgatat caagaagagt tgaccgtgtt tatatggaaa
    91381 ataatgagct tgtacaaata aacagttatt tgctaaaata aaaccatgat attataatct
    91441 gggaggcgat tttgtgtacg ataagagtaa agaaaaaata gaagttactt tacctgactc
    91501 tctgatttct gaagttgata gtatagttca gatggagaac agtaacagag aagattttgc
    91561 aaaagctgct tttcagttct atataactca gaaaaagaaa attcatatta aagaatctat
    91621 gaaaacgggt tatagagaaa tgggtcaaat taatctgtca ctagctgaat taggtatgac
    91681 agttgaagaa gtatcttctg atgataaaaa tgaagggaag attgcccaag gtgagtacta
    91741 attgtgaaat aaagcgtggt gatttatatt acgctgattt aagtccagtt gttggttcag
    91801 agcagggagg cgttagacct gttctgataa tacaaaatga tgtaggtaat aaatatagtc
    91861 ctacagttat agtatcagct ataacatctc agattaataa agccaagtta cctactcatg
    91921 tagagataag ttccaatgaa tatgggctta ataaagactc agtaattctt ttagagcaaa
    91981 ttagaactat cgataaaaag agactgaggg aaaaaattgg ttgtctagat gaaaatatga
    92041 tgataaaagt agatgatgga cttcaaataa gtttaggatt gtttactttt taaagaggga
    92101 tttgtctttt attagacaat cccttttttc ttttaaatat ctcttaaggt ttaattttaa
    92161 tgtactatgt taataaaatt aaaaataacg caaaaaaata gcataaaagt gatataataa
    92221 tatggaattg atataaaatt acgaactcaa gggaggtaat tgcatgagag accataaagg
    92281 attaaatgag attgcgcgta tcataagaag agatatagtt tcaatgatac atagagccaa
    92341 atctggacat ccaggagggt cattatcagt agttgaaata cttactgcgt tatactttga
    92401 tgaaatgaat gtggattctt caaatcctaa aatggaggat agagatagat ttgtattatc
    92461 aaaaggacat gctgcaccag cattatatgc tacattggct gaaaaaggtt attttgataa
    92521 ggaagaactg aatggattaa ggaagatagg aaggatgtta caaggtcatc cagacatgaa
    92581 aggcacgcca ggtgtggaaa tatctactgg ttctttaggt caaggttttt cagttgcatg
    92641 tggtatggct atggcatcaa agttggataa tgctccgtgg aatgtgtata ctttgcttgg
    92701 tgatggtgag gttcaagaag gaattgtttg ggaagctgct atgagtgctg cacattataa
    92761 acttgataat ttaattgctt ttttagataa taatggactt caaatagatg gagatataga
    92821 aagcgtaatg agtttaggtt caatagttga taaatttaaa gcttttggtt ggaatgtaat
    92881 tgagatagat ggacatgatt ttgaccaaat atttgcggct cttgatatag ctaaatcaac
    92941 agttaaaaaa cctacaatga tagttgcaaa aactataaaa ggcaaaggaa tatcttttat
    93001 ggaaaatcaa gcaggatggc atgggacagc tccaagtgat gaggagctag aaaaagcctt
    93061 gcttgaatta ggaggtgctg acaatgagta aaatggcgac gagagaagca tatggtaaag
    93121 cattagtaaa attgggtcaa ataaatgaca atgttgtagt attagatgca gatttatcaa
    93181 agtctacaaa aacacatgat ttttacaagt catttccaga tagatttttt aatatgggta
    93241 tagctgaaca aaatttaata ggcgctgctt gtggattatc aactgctggt aaaatacctt
    93301 ttgcaagcac ttttgctatg tttgctacgg gaagggcttt tgagattata agaaattcag
    93361 tttgttatcc aaagctaaat gtaaaaatat gtgctactca tgcaggtctt actgttggag
    93421 aggatggagc atctcatgaa agtgtggaag atattgctat aatgagagct ataccaaata
    93481 tgacagttct tgttccagct gatggagttg aaacagaaaa aattattttt gaaatagcta
    93541 agtataatgg acctgtttat gttagattag ggagaagttc agtaccagtt ttatttgatg
    93601 aagattataa atttgaaata ggaaaaggta cagtcttaag agaaggaaag gatgtatcaa
    93661 ttatagcttg tggaattatg gtaaatgaag ctctattagc acaagaaaag ttgcaagaag
    93721 aaggtataag tgctagagtt ataaacatgt cctcgataaa acctatagac aaagatttaa
    93781 tattagaatc agcaaaagaa acgaatgcta ttgtaactgt agaagaacat agcataattg
    93841 ggggattagg ttctgctgta agtgaggtag taggagagtc ttgtccaacc attgtaaaga
    93901 aagttggaat taaagataca tttggagaat ctggtactcc aaatgaattg ttaaaaaaat
    93961 atgaattaac ttgtgatgac ataataaaga ctgttaaaga agctattatt gcaaaaagaa
    94021 tgtaagagat ataaattatt catgtttgtt aataatataa tatatttaaa agagaggtat
    94081 aaagtgttat attaatttat atctctcttt tttttatatg atatcaatat atcaaaaatg
    94141 taataaaata ttcaaatttt tacaaaaata gagaaaatat tcaaatatat attgactatt
    94201 cagaaaaata tgactaatat ataatttata gagaaactaa ttttatatat aatgactact
    94261 tagataaaag aatctaaaaa aattatgaga ggaggagtag ggtataaaat cagattataa
    94321 gttttaagtt attatactaa aaagctataa aacagttatc atgtatttta tgttaaagga
    94381 aattgtaggt agtttatcga attgtttaat cagaagaaaa aatttcacat ggggaggtaa
    94441 ctgagatatg tcagaaaaag taaatgttaa aacagtcatc agtttcgcag gtgcgtatgt
    94501 agcaacagta atcggttctg gatttgcaac aggacaggaa ataatgcaat tcttctcttt
    94561 ttatggtttt gcaggtatta ttggtgggat aatttcaatg gtattattct catggatggg
    94621 tgcttcagtt atgtcaaaag gtaaagagct tcaacttaaa gaacctatca aaatttatcg
    94681 tgtttattgt ggtaagtatt taggaatatt ctttgaatgg tttggacctt tattcttatt
    94741 tggtgttttc gtagtaatga tttcaggagc aggagcaact ttaactgaat actatggatt
    94801 aaatccattt gtaggtagag taggtatggc agtagtagca ttattaactg tttcactagg
    94861 tcttgataaa ttatcaaaga tactaggtgg aatagggcct ataataataa tctttacact
    94921 tttagttggt ggtataagtt tagctaaaaa cataggtaat ataggagaag ctgcacaagt
    94981 tcttagcact gtaaagatag ctaaaccagt tccaaatgcc tatctatctg gtgtaatata
    95041 tactacttat aacacaattg ttgttatggc attcttaact ggattaggag caagtgctgc
    95101 taacaaaaaa gaagctattt gtggtggtat attaggtgga gtagcactta tggctgcagc
    95161 tataatgatg cacttagcta ttctttctga tataggaaac ttatattcaa aagcaatacc
    95221 atcattattc ttagcagata agatttcacc agtaattgga gttttattct caataattct
    95281 tatattaggt atatatacaa cagctgtacc tttattatgg tcagtaacta atagatttgt
    95341 agatgacaaa catcctaagt ttaaattaat aactttaata gcagctatat taggtcttat
    95401 aggtggcttc ctaccatttg ataagctggt tggtatttta tatccatata ctggatatat
    95461 gggcgtcata atactggttt gtgttcttta tagacagatt acaaaaacat ctggatatca
    95521 agaaggaatc agtgacaaat aagtattttt taatattgta aactcaagag ctatatgaaa
    95581 acaatgaaaa tgttttcata tagctctttt tattttttag ctcgtttatt atgaaataga
    95641 aaaaacatgt tttttagtta cattacatat atttataatt atgtataaat atatactaat
    95701 caataattta gagtctaaga taagtttata aatcatatat ttagaatata tgggaggagt
    95761 gataagattg aatgtaaagt ttaatattaa aggtataatt tatggagtta tagctcttgt
    95821 attcgttata gggggattta tattcatacc aagtatgttt gtagagaaat ctaaacccat
    95881 agattataca gtattacaaa gaaattcaat accagaaaag atattagatg tgatggataa
    95941 gtatacaaat gaagaaagag cattagcggc taaaatcgat aacaaaatat acattatagt
    96001 gactagagga aataataaat atggtataga aatggacaaa atagaaagtg tggttgagga
    96061 aggaaaggat gttttgagag tcaagataaa gtataaagat aaggaagatt cttttccata
    96121 catagttgtt gaaacaaata tgagtgaact tccagataga atagaattaa atgcgacgaa
    96181 ataagaaggt attttataat taaataactg catattgtac aataaagtca tggtttttat
    96241 taatgtaagt aaattgtgta tggttcttat tgttgtaaaa gccatgactt aaaatgctta
    96301 taaagtaaga tattaacagt agtatttatt actacattaa atggggtctg gaaatactta
    96361 atattctaac tatttggagt gataattcta gtttcagtaa ctatgtagtc taattgtgca
    96421 tcatgagttt cttttggaat agaatcgaat atctggaagt caaatgctat gccgatagtt
    96481 atagcatctt ttcttaagtt ttctaaaaat ctatcataaa atcctccacc atatcctaat
    96541 ctatagcaat gtatatcata tgctacagct ggtacaatta caacatctaa ggccttaata
    96601 tcaattgtag gtgatttttc gttaggttct cttattccat atgctccaat tctcaatccc
    96661 tcttttaaat ctgttatttg agtagggatt aatgtatgat tttcttttat tgtgatggga
    96721 cttgatacta ttttctttag agacaaaagt tttttgatta aattatctgt ttgaacttca
    96781 ttgttaaaat ttagataaag cattatatta gttgcatttc ttatatcttc catttgaagt
    96841 aatttttcag ttattaaatt tgaattacta agtacaaagt ttgcattctt attttttctt
    96901 gattcaataa ctttttttct aaattctttt ttcataaaat aattcctccc ttaatatgtt
    96961 ataatgtgta cagcaattgt atttaatata aattttagta taatttattg taataaagca
    97021 atatatttgg gtaaatgtaa taaaaataac agctagtcat ataataataa tagtttatgt
    97081 aaaaggagat gtatcttgtg atttcaaaga agaaagctat ttttttaggg gtgattttag
    97141 taattataac agctatggtt acatcagcat ttcagttgac attggggaat aaagttgtaa
    97201 tatcgaaaga actctatgaa gactataaaa aatatgataa gttgttagga ctagaatcta
    97261 ttataaaaca agatttttat aaaaaagtat cagatactga tttggtaaat ggagcagcaa
    97321 aaggtctatt tctaggaaca aatgataagt attctggtta ttatactaaa gatgaaatgg
    97381 agaatctaat taatgatagt gaaggttctt atgttggtgt aggtatgtat ataggggcat
    97441 caaaagatgg tggcttagta gtagttccca tgaaagattc tccagcagag aaagccggtg
    97501 taaaatcagg ggataaatta gtaaaagtca atggaaaatc tgtctcatat aaaaattcag
    97561 atgaagctgt tcgtatgatg aagggtaaaa aaggtaaaac agttgaactg actatattaa
    97621 gagaagataa acaattgaac tttaaagtaa aaacagacca gataattgag aagagtatag
    97681 aaagtaaagt aattgataat gatttaggat atatagaaat tacccaattt atatcaagta
    97741 catatactga ctttgataaa gctttaaaag aattaaaagc aaaaaatatc aagggattag
    97801 taatagacct tagaaataat cctggtggaa tgctagatat atgtaaagaa gttgctgatg
    97861 agttaattgg tgaaggaact atagtatata caaaagataa taagggaaat actgagtact
    97921 tgaaatctga taaagaaaaa ttgggacttc caatagtagt gttaactaat ggagaaagtg
    97981 cttctgctgc tgaaatatta acagctgcaa tcgttgataa taaagaaggt atttctgtag
    98041 ggactacaac ttttggtaaa gggttggtac aatcagttgt aagattgaag gatggaacag
    98101 gatacaagct aactactgct caatatttta cacctaatgg ggattatatc aatgaaaaag
    98161 gtataaagcc tactattgaa gagaaagatg aaaataaaca gttagatgta gcaactaagt
    98221 ggcttagaga acagattgat aaataggatt taatttttat aaacttatac tataaaataa
    98281 agaacattat aaaagagatg tctcaaaatg aactttttag ttcatgaggg agtctggaaa
    98341 tatttatgtg tattctttaa ataaaaattt attttttaag tgataattaa atttttacag
    98401 acagggctat ggttttaaaa atcatagcct tattttgata ttttttaaaa acatattgca
    98461 acccagttgt tccaaatatt atatcattta cttttaaaat atcgcaaact gcatattata
    98521 tattcattat aatataattt atctaactag aacctcagtt tactaattaa attagatgca
    98581 tagaataact tttttaaaca ttgaaaataa attaaactct aaacacattt aaatataaat
    98641 ttatatatca ttttgacttg gttaaacaat ttcatactt
//
LOCUS       DAMKMI010000002        80066 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMKMI010000002 DAMKMI010000000
VERSION     DAMKMI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 80066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.002"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(292..705)
                     /locus_tag="PC429_000100"
     CDS             complement(292..705)
                     /locus_tag="PC429_000100"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF3845631.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(746..1876)
                     /gene="rodA"
                     /locus_tag="PC429_000101"
     CDS             complement(746..1876)
                     /gene="rodA"
                     /locus_tag="PC429_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF3845632.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(1961..2245)
                     /gene="minE"
                     /locus_tag="PC429_000102"
     CDS             complement(1961..2245)
                     /gene="minE"
                     /locus_tag="PC429_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF3845633.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2263..3060)
                     /gene="minD"
                     /locus_tag="PC429_000103"
     CDS             complement(2263..3060)
                     /gene="minD"
                     /locus_tag="PC429_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF3845634.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(3084..3767)
                     /locus_tag="PC429_000104"
     CDS             complement(3084..3767)
                     /locus_tag="PC429_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF3845635.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3865..6843)
                     /locus_tag="PC429_000105"
     CDS             complement(3865..6843)
                     /locus_tag="PC429_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF3845636.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6855..7343)
                     /gene="mreD"
                     /locus_tag="PC429_000106"
     CDS             complement(6855..7343)
                     /gene="mreD"
                     /locus_tag="PC429_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF3845637.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7358..8251)
                     /gene="mreC"
                     /locus_tag="PC429_000107"
     CDS             complement(7358..8251)
                     /gene="mreC"
                     /locus_tag="PC429_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF3845638.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8261..9271)
                     /locus_tag="PC429_000108"
     CDS             complement(8261..9271)
                     /locus_tag="PC429_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF3845639.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9392..10051)
                     /gene="radC"
                     /locus_tag="PC429_000109"
     CDS             complement(9392..10051)
                     /gene="radC"
                     /locus_tag="PC429_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF3845640.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10096..10680)
                     /gene="maf"
                     /locus_tag="PC429_000110"
     CDS             complement(10096..10680)
                     /gene="maf"
                     /locus_tag="PC429_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF3845641.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(10900..11871)
                     /locus_tag="PC429_000111"
     CDS             complement(10900..11871)
                     /locus_tag="PC429_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF3845642.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11887..12462)
                     /gene="rsxA"
                     /locus_tag="PC429_000112"
     CDS             complement(11887..12462)
                     /gene="rsxA"
                     /locus_tag="PC429_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF3845643.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12477..13070)
                     /locus_tag="PC429_000113"
     CDS             complement(12477..13070)
                     /locus_tag="PC429_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF3845644.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(13083..13652)
                     /locus_tag="PC429_000114"
     CDS             complement(13083..13652)
                     /locus_tag="PC429_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF3845645.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13652..14629)
                     /locus_tag="PC429_000115"
     CDS             complement(13652..14629)
                     /locus_tag="PC429_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF3845646.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADISSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14652..15989)
                     /gene="rsxC"
                     /locus_tag="PC429_000116"
     CDS             complement(14652..15989)
                     /gene="rsxC"
                     /locus_tag="PC429_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF3845647.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16205..17149)
                     /locus_tag="PC429_000117"
     CDS             complement(16205..17149)
                     /locus_tag="PC429_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845648.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17260..18534)
                     /locus_tag="PC429_000118"
     CDS             complement(17260..18534)
                     /locus_tag="PC429_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF3845649.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18666..19037)
                     /locus_tag="PC429_000119"
     CDS             complement(18666..19037)
                     /locus_tag="PC429_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3845650.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19265..19864)
                     /locus_tag="PC429_000120"
     CDS             complement(19265..19864)
                     /locus_tag="PC429_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845651.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(20065..20277)
                     /locus_tag="PC429_000121"
     CDS             complement(20065..20277)
                     /locus_tag="PC429_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF3845652.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(20350..22065)
                     /locus_tag="PC429_000122"
     CDS             complement(20350..22065)
                     /locus_tag="PC429_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3845653.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNITSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(22065..22619)
                     /locus_tag="PC429_000123"
     CDS             complement(22065..22619)
                     /locus_tag="PC429_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF3845654.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(22612..23214)
                     /locus_tag="PC429_000124"
     CDS             complement(22612..23214)
                     /locus_tag="PC429_000124"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF3845655.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23223..25871)
                     /gene="polA"
                     /locus_tag="PC429_000125"
     CDS             complement(23223..25871)
                     /gene="polA"
                     /locus_tag="PC429_000125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF3845656.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            26023..26110
                     /locus_tag="PC429_000126"
     tRNA            26023..26110
                     /locus_tag="PC429_000126"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26057..26059,aa:Leu,seq:caa)
     gene            complement(26297..26740)
                     /locus_tag="PC429_000127"
     CDS             complement(26297..26740)
                     /locus_tag="PC429_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845657.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(26743..26940)
                     /locus_tag="PC429_000128"
     CDS             complement(26743..26940)
                     /locus_tag="PC429_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3845658.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(27132..27998)
                     /locus_tag="PC429_000129"
     CDS             complement(27132..27998)
                     /locus_tag="PC429_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3845659.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEAAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(28224..29093)
                     /locus_tag="PC429_000130"
     CDS             complement(28224..29093)
                     /locus_tag="PC429_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3845660.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(29599..29820)
                     /locus_tag="PC429_000131"
     CDS             complement(29599..29820)
                     /locus_tag="PC429_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845661.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(30322..31302)
                     /locus_tag="PC429_000132"
     CDS             complement(30322..31302)
                     /locus_tag="PC429_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845662.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(31466..32338)
                     /locus_tag="PC429_000133"
     CDS             complement(31466..32338)
                     /locus_tag="PC429_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3845663.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(32502..33053)
                     /locus_tag="PC429_000134"
     CDS             complement(32502..33053)
                     /locus_tag="PC429_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3845664.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(33073..33975)
                     /locus_tag="PC429_000135"
     CDS             complement(33073..33975)
                     /locus_tag="PC429_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF3845665.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDD
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(33990..36362)
                     /locus_tag="PC429_000136"
     CDS             complement(33990..36362)
                     /locus_tag="PC429_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF3845666.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(36583..37272)
                     /locus_tag="PC429_000137"
     CDS             complement(36583..37272)
                     /locus_tag="PC429_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845667.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVYDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(37513..38892)
                     /locus_tag="PC429_000138"
     CDS             complement(37513..38892)
                     /locus_tag="PC429_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3845668.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(38886..39599)
                     /gene="rgbR"
                     /locus_tag="PC429_000139"
     CDS             complement(38886..39599)
                     /gene="rgbR"
                     /locus_tag="PC429_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF3845669.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(39849..40409)
                     /locus_tag="PC429_000140"
     CDS             complement(39849..40409)
                     /locus_tag="PC429_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF3845670.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(40505..41308)
                     /gene="zupT"
                     /locus_tag="PC429_000141"
     CDS             complement(40505..41308)
                     /gene="zupT"
                     /locus_tag="PC429_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF3845671.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(41459..42622)
                     /locus_tag="PC429_000142"
     CDS             complement(41459..42622)
                     /locus_tag="PC429_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3845672.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(42682..43869)
                     /locus_tag="PC429_000143"
     CDS             complement(42682..43869)
                     /locus_tag="PC429_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845673.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(44232..45932)
                     /locus_tag="PC429_000144"
     CDS             complement(44232..45932)
                     /locus_tag="PC429_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3845674.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(46187..46462)
                     /locus_tag="PC429_000145"
     CDS             complement(46187..46462)
                     /locus_tag="PC429_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3845675.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(46581..47153)
                     /gene="mobB"
                     /locus_tag="PC429_000146"
     CDS             complement(46581..47153)
                     /gene="mobB"
                     /locus_tag="PC429_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF3845676.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(47137..48318)
                     /locus_tag="PC429_000147"
     CDS             complement(47137..48318)
                     /locus_tag="PC429_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF3845677.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKFSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(48529..49263)
                     /locus_tag="PC429_000148"
     CDS             complement(48529..49263)
                     /locus_tag="PC429_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845678.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            49625..50530
                     /locus_tag="PC429_000149"
     CDS             49625..50530
                     /locus_tag="PC429_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3845679.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(50690..51532)
                     /locus_tag="PC429_000150"
     CDS             complement(50690..51532)
                     /locus_tag="PC429_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3845680.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(51516..52274)
                     /locus_tag="PC429_000151"
     CDS             complement(51516..52274)
                     /locus_tag="PC429_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3845681.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(52291..52761)
                     /locus_tag="PC429_000152"
     CDS             complement(52291..52761)
                     /locus_tag="PC429_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3845682.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(52761..53504)
                     /locus_tag="PC429_000153"
     CDS             complement(52761..53504)
                     /locus_tag="PC429_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF3845683.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(53508..53921)
                     /locus_tag="PC429_000154"
     CDS             complement(53508..53921)
                     /locus_tag="PC429_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3845684.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(53947..54699)
                     /locus_tag="PC429_000155"
     CDS             complement(53947..54699)
                     /locus_tag="PC429_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3845685.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(54939..56333)
                     /locus_tag="PC429_000156"
     CDS             complement(54939..56333)
                     /locus_tag="PC429_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF3845686.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(56353..56562)
                     /locus_tag="PC429_000157"
     CDS             complement(56353..56562)
                     /locus_tag="PC429_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF3845687.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(56591..58036)
                     /locus_tag="PC429_000158"
     CDS             complement(56591..58036)
                     /locus_tag="PC429_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3845688.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            58146..58940
                     /locus_tag="PC429_000159"
     CDS             58146..58940
                     /locus_tag="PC429_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF3845689.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            58991..60313
                     /locus_tag="PC429_000160"
     CDS             58991..60313
                     /locus_tag="PC429_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF3845690.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(60413..61630)
                     /gene="cdeC"
                     /locus_tag="PC429_000161"
     CDS             complement(60413..61630)
                     /gene="cdeC"
                     /locus_tag="PC429_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF3845691.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(61813..61993)
                     /gene="ssrS"
                     /locus_tag="PC429_000162"
     ncRNA           complement(61813..61993)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC429_000162"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(62108..63013)
                     /locus_tag="PC429_000163"
     CDS             complement(62108..63013)
                     /locus_tag="PC429_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845692.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            63225..63623
                     /locus_tag="PC429_000164"
     CDS             63225..63623
                     /locus_tag="PC429_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845693.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            complement(63746..64762)
                     /gene="ccpA"
                     /locus_tag="PC429_000165"
     CDS             complement(63746..64762)
                     /gene="ccpA"
                     /locus_tag="PC429_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF3845694.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(65015..65215)
                     /locus_tag="PC429_000166"
     CDS             complement(65015..65215)
                     /locus_tag="PC429_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845695.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            complement(65249..65428)
                     /locus_tag="PC429_000167"
     CDS             complement(65249..65428)
                     /locus_tag="PC429_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845696.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(65610..65816)
                     /locus_tag="PC429_000168"
     CDS             complement(65610..65816)
                     /locus_tag="PC429_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845697.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(65864..66289)
                     /locus_tag="PC429_000169"
     CDS             complement(65864..66289)
                     /locus_tag="PC429_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845698.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            66461..66688
                     /gene="acpP"
                     /locus_tag="PC429_000170"
     CDS             66461..66688
                     /gene="acpP"
                     /locus_tag="PC429_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF3845699.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(66832..67836)
                     /locus_tag="PC429_000171"
     CDS             complement(66832..67836)
                     /locus_tag="PC429_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF3845700.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(67850..68752)
                     /locus_tag="PC429_000172"
     CDS             complement(67850..68752)
                     /locus_tag="PC429_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF3845701.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            complement(68948..70123)
                     /locus_tag="PC429_000173"
     CDS             complement(68948..70123)
                     /locus_tag="PC429_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF3845702.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            complement(70210..71055)
                     /locus_tag="PC429_000174"
     CDS             complement(70210..71055)
                     /locus_tag="PC429_000174"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF3845703.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(71153..71950)
                     /locus_tag="PC429_000175"
     CDS             complement(71153..71950)
                     /locus_tag="PC429_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF3845704.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(71969..72979)
                     /locus_tag="PC429_000176"
     CDS             complement(71969..72979)
                     /locus_tag="PC429_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF3845705.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(72998..73780)
                     /locus_tag="PC429_000177"
     CDS             complement(72998..73780)
                     /locus_tag="PC429_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF3845706.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(73839..74975)
                     /locus_tag="PC429_000178"
     CDS             complement(73839..74975)
                     /locus_tag="PC429_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF3845707.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(75684..76151)
                     /locus_tag="PC429_000179"
     CDS             complement(75684..76151)
                     /locus_tag="PC429_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF3845708.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(76230..76772)
                     /locus_tag="PC429_000180"
     CDS             complement(76230..76772)
                     /locus_tag="PC429_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845709.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(76824..77510)
                     /locus_tag="PC429_000181"
     CDS             complement(76824..77510)
                     /locus_tag="PC429_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF3845710.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            complement(77510..78370)
                     /locus_tag="PC429_000182"
     CDS             complement(77510..78370)
                     /locus_tag="PC429_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3845711.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            78505..79305
                     /locus_tag="PC429_000183"
     CDS             78505..79305
                     /locus_tag="PC429_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF3845712.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            79330..79899
                     /gene="yfcE"
                     /locus_tag="PC429_000184"
     CDS             79330..79899
                     /gene="yfcE"
                     /locus_tag="PC429_000184"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF3845713.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
BASE COUNT        25383 a        14658 c         8486 g        31539 t
ORIGIN      
        1 ttttattaat gttgtcataa caacatttac ttggttgaaa tagtattacc tataagttta
       61 aatatatata ataatagact tgattctatt tcatattatt aaaatagagt aatagtttaa
      121 attctactac tctatctata atctatttcc taattattta atctcaaatt tagctcttca
      181 tcatttaaat ctttcataag atttctacag gtactagatt tattccaaca gaaagactag
      241 ggcattaaac acttatatat ctgtttcttt agactgtaat attaatattt tttaaatacg
      301 ttgacttttg ctttttctaa cttctctcca tgatagttct cctctatcaa gagcttttat
      361 aaatatttct gctgaagcta gatttgtagc tattggaaca tgataaacat cacaaagtct
      421 tattaacgct tgtatatctg tttcatgagc ctgagatgtc aatgggtctc ttaaaaaaat
      481 aactaaatct atttcttgtg ttactattaa agaacctatt tgttggtctc cacctaaagg
      541 accagatgct aatctattta tatttaattc agtttcatcc attattcttt ttcctgtagt
      601 tccagttgca tatagtccat acttttttaa aattgattca taacctatac aaaatccgac
      661 catagtgttt ttcatttggt catgtgctac taatgctata ttcatatatc taatctcctc
      721 aatttatgtt tagatgtatt taaatttaga aatttatttt ctttcttctc atacacacat
      781 ttagtactaa tcctaagttt gctaaagatg tcaataacga acttcctcca taacttacaa
      841 aaggtaatgt tactccagtt actggtatta acgcaaccgt catacctata ttttggatta
      901 tctggtaacc aaacattccc ataacaccaa ccactatgag ggttccataa aaatcttttg
      961 catctcttgc aattgctaaa agccttaata ggaatatcat aaataagatt atcaacactg
     1021 ccattcctat tacaccaagt tcttcaccta caacagcaaa tataaaatca ctatcttgta
     1081 caggtaaaaa atcttcttga ttttgacttc cattgtaaag tcctttacca gttacgcctc
     1141 cagaaccaat tgcaatcaga gattgcataa cttgatagtt tccttttaga gttacatctt
     1201 ctggatttag aaaagcttct attcttacct tttgatggtc tgccatcatt aaatacatca
     1261 gtggcattga cactaatagt atgattattc ctctttttat caactttgag ctaagacctg
     1321 ctgtgaacag cattgcaaat atcatacaac aaaatacaat tcctgttccc aagtctggtt
     1381 gtgcaatcaa taatccgata aatggaagag aataaactac aacaggcata acttccttta
     1441 acgtatttaa tttatccttt ttggattcaa gtatctttgc ataacttagt acaaatgtta
     1501 atttgactat ttcagatgtc tgcaagtcaa gtggtcctaa gttaatccac gacctagctc
     1561 caccttttac tgtcccaatt ccaggtaata aaactatggc taacaaaata agacttatta
     1621 tataaagcgc tttataatac ctgcctagaa ggttatagtc aaaaaataat atgactataa
     1681 tcattcctat cccaagtaca aaagctaatc cttgtttaat caattgatta tatgaacctg
     1741 ttgaattagc atgtgttgca ctactaagta taacaagccc aaaaataaag atagctaata
     1801 ctgtaactat aagtttccag tctaactgtt ttatcaactt aattgtcgac ttataattaa
     1861 tattgatttt tttcaaagaa tcaccgtctt tctatcttta aacatataat taaataaaag
     1921 gggcatatac caataaggat atcccccttt taagcactaa ttacatacat ctatctttta
     1981 tattctttag tggtatatta gcaactaatg cagatacagg tttgtcatca tgctcacctc
     2041 tgcatttaga catttttatt tcaagaccat catcttctat ttctgcataa ttagctatta
     2101 cttttaaaat gtctgttttt atcatctcta aaagttgtgg tgaacaatct actctatcat
     2161 gaactaaaac tagctttaac ctctctttag caacagattt actagtttta gcctcgttag
     2221 aaaaaactct aaataaatct aacacagatg tatcctcctt tgctacttag ccatgctaaa
     2281 cattttttta agtctaccaa agaatccatc ttcaacttca agatcaagta gaggaacttc
     2341 ttcatttagt atcctttttg cgatattttt gtatgcttga ccagcaagtg acttagaatc
     2401 aagtatagct ggctctcctt tatttgttga tataattatg ctttcatcat caggaacaag
     2461 acctaacaaa tctattgcta atatttctat tatatcttgt ttatccatca tgtctccacg
     2521 cttaaccata tcatttctaa ttctatttat aactaatctt atctctttta tttcatttgc
     2581 ttctaataga cctattattc tatcagcatc tcttactgct gatatttctg gattagtaac
     2641 tacaatagct ctatctgccc cagctactgc atttttaaag ccttgctcaa tacctgctgg
     2701 acaatctatt attatatact cgaaagattc ttttagtttc tcacacaggt caatcatctg
     2761 ctcaactgag acagcatttt tatctctagt ttgtgctgct ggtaataaat atagattatc
     2821 aaatcttttg tcttttatta gagcttgctt aagcctacaa gttccttcaa ctacatctac
     2881 tatgtcataa actattcgat tttcaagacc cattactaca tctaagtttc taagtccaat
     2941 atctgcatcc acaactactg tttttttatt ttctaggctt agcgcagttc ctaagtttgc
     3001 tgcggtagta gtttttccaa ccccaccttt accagatgtt ataactataa cttcgctcat
     3061 aatactaccc cctatttttc tttctatttg tctaactttg acaaatagct ttcgattaca
     3121 attcttccat tacttacaaa ggctatttct gggctaattt ctgattcact ttcataattt
     3181 tcttcatcat ctggagcttc tgcaatatta tctgctattt gtaatatttt aggattaaga
     3241 tttccagcaa caacatatgc aaattcattg ccttctgcac ctgcatgtac aaaaccttct
     3301 attttaccca ttacaaaaac attacctcct gctacaacct tagcaccagc atttatatct
     3361 cccataacaa ctgtatttga ttttgaaagg acttttgaac caggtttcat atcattcaat
     3421 ataactacat caccttcaaa ttcaatattt tcaccagatc ttagggtatt gacatatttt
     3481 gttggaaaat tactattttc ttcaattttt tgatcttcaa caaattccac atcaaatcta
     3541 gaagtaattc cttctttaag ttctagaatc tccatgtcag ttaaacaatc actattaatt
     3601 ttactaattt tagccccatt aaaaaagcca acatatgctt ccaatttgtt tattattttt
     3661 tcctgtattt cctcaaatgg agcttctctt tttatattta ctattatccc tcttttatta
     3721 cccttgaact ctactagttc ttgactacaa atttctctta aagacataac aacctccaga
     3781 gtgatatatc atttatattc ttcaaatacg ctttaaaccc ttttattttt tctatataaa
     3841 gctaaagttt atacatagat tgagctatct attaatttgt gactcgaaat ttatattttc
     3901 gtcagaagaa ttgttaacat cattcgtctt gttgtcattt ttactattag ttggctctaa
     3961 tcccatataa gttccaataa cttctctagt tggtaaaagt gagaatacac ttgaatcacc
     4021 ttgaggaatt acacatacaa cagctatttc tgggtcatct gctggagcaa aagcaacagt
     4081 ccacgtaaaa gaaccataat cttgtttaaa tctatctatt tggtcatctg ttatttttgg
     4141 atttaattct tttatagctt ttcttagata tgaagaattc accttatcag tgtcctctaa
     4201 tctaactttt actccatctt ctaattcttc ctctaatttc tttctttctt cgtctttcaa
     4261 atctttattt tctaatttct tttttatttc cttttcctta gctaatgaaa gctctttttc
     4321 tttttctgct ttcatcttat cagctagttt aatcgcatca tttaggttga cattataaga
     4381 tgacatatga ctttttaaat attcatactc gttatctgta ggtattttcc cacttttctc
     4441 agctgtacca gtttttgctg ctacatctat tggaaaatca gcaaaagcac tcttcgctgt
     4501 accttgtctt gcaaccaatt tcataccttt tgttaattct tttaagttat ctggattttt
     4561 aaaaggtatt ttttcgactt tcttctggtc attaatttta actgatgaat aatcacttga
     4621 aacagctcta tcaactacag ataattctac taaattacct ccatttgcta tagcagctac
     4681 atatcttgat atttgagctg gtgtataagc attttcacct tgtcctatag ctaagttgaa
     4741 tgtatctgca gtactccatt ttgcaaaatt aaaataacta aatactgcta aatctgctac
     4801 ttcttcaatt ctatcttctt taacatccaa tttttttagc ctattcattg tttcaactct
     4861 accaggagtt ttcttttctg ctgcccagct tacaatctca ttgattcttt tctcatattc
     4921 tttaggattt ttttctctag ttatatcaac aaaatcattt gccatttctc tttctaaatt
     4981 tgactttaga agagcttgtg ttgatttcaa tttatcttct gtgctaggaa ctttacctgc
     5041 tctttcttct acttcatctt gaagccctgt attttggtca agcccaaata attttgcata
     5101 ctcaagtatt ttatttgcat tcatatctat accaatgctt tttccatctg gccatgtttt
     5161 acctgtacca attgttgcca tatatatatt acaagactct tgtattgctt tatataagtt
     5221 tgtcatacca tggtttcctc taccatggtt ccatacatag tcaccaaaag attttttacc
     5281 taacattata accccagggt catttatagt aaagtttgga tccagaccat tttctaacgc
     5341 tgccattgaa gttaccatct taaatgaaga acctggctga aattcccctt gtgtaaccaa
     5401 gtttaaaagt ggacttcctg ctagtaagtc atttggattt tttggttgaa gtgctttata
     5461 atcctctgta gaaatacctt ttgcaaattt atttgggtca taattaggat aacttgatga
     5521 agctaatact tctccatttt taacatcaat agctataaga gctgctgact gtgcttttcc
     5581 tgcatatgca ctaattggtt tgtccccaaa tttactttta aaagtacctc ctttagaagc
     5641 aacttcgatt atctgtttta atgcattatc tgaaacttct tgtaaatctt tatctagtgt
     5701 aagatacaca gtatctcctg atttaggttt tttagattca atttctttac tgattctacc
     5761 caatgcatca actttaacca ttttataacc atcagttcct cttaatctac tttcattact
     5821 cttttctact cctgcaagac ctaccatatc tccagtttca taaccttttt gaaggtatga
     5881 ttctatttga gtggatggca tttttcctac atatcctaac atatgagaag ctaatgaccc
     5941 attaggataa tatcttactg gttctactgc tattgaaaca ccaacaaggt ccatagcact
     6001 ttcttcaatt tgagcaatcg tagtttctcc cacatcctta gcaatagtta ctggattata
     6061 ttgagaatat ccttttgatt ttatcaaatc ccttacgacc attatttttc ttgcatcttc
     6121 atcactcaaa cttttatcaa tttctaatgc atcactattt cttacttttt caaacgcttc
     6181 tttggcactc agtttagttt cttttatctt ataactagcc aaccaatctc tcttatctct
     6241 ttctgctgtg aacatccatt tactaactaa aataggtgga taatatccat tttcatttag
     6301 ttttgcctgt aatcttgtcg catccaatct tttatcttct tgagataata ttccagctga
     6361 tataagtttg tccaccaagt aataaaaact ttccttagca ttataatcat ttggaatacc
     6421 attttctgat ttatattccc taatatctct gtcatatgta taatagtact ttccattttc
     6481 tacatatata ggaaactcat ctacatattc ttcaccattt ctctccagca aatttataag
     6541 ttttaatgat atttcatttg ctcttgaatg cttatttgca tctttcttat taatatcgtt
     6601 acctgaaact tgaacagtaa ataagttttt atttcctgcc aacagtcttc catatctatc
     6661 ttttatttct cctcttggag cttctattgc caacttttta tatgttttat tctcagctaa
     6721 ctcattgtaa tattcatatt taaatgtagt catatagatt atttttacta aaatagctaa
     6781 aaatatcacg cccatgacta tcttaattat ttttagtcta tcaacttgtt tattttcttc
     6841 catgaaatca ccaactaatc ttctttaagt tttaaaactg ctcttttaga taagcgatat
     6901 attactaaac caattaaact attcactatt ggtgctacta gtagaccttt tacaaaaagt
     6961 gttgatatgt tgtagctttg ataaattata ctagatgtac ctatattgac aattgaatca
     7021 attaaactgc ctataaaaat taaaacgaaa gtaataatat aactaccttt atatagctga
     7081 tctctcatgt atccaattgc atatgcaata ataaacaaaa ttaatgcatt tacaccaaaa
     7141 attccaccaa cggttatgtc tttaataagc cctattattg ctgctataat cccaccttct
     7201 aattcatcta aataaagaga gattatggtt acatatataa gaactaaatt aaagcttatg
     7261 ccaaaaatat ctatataatt tgttatacta ttttctatag tcataagcac aatccccaac
     7321 agacaaagta aaactttttt cattgttttt atccccttta tcctatattt cttggttcta
     7381 tgactacaac gtcatttata ttcttaaaat caacataagg ttttacaact acatatttta
     7441 atgacttatc tttatcatca accacttttt caacttcgcc aattggtata ccatctggga
     7501 agaatccaag acctgatgtt gttattatat ctccttgaat tacattatat gatgagtcaa
     7561 acatatatcc ttgcaaatat cctttactat tataactatc tttattatct aaagtagtat
     7621 tttgagtaat tgtaccttta gcattggcat cctttgctag tttaaagctt acagatgcct
     7681 tagaatccaa tagtgaaatt gccttactat aattgttgga aacctcataa actatgccaa
     7741 ctaatccact accattcata actatacttt ctttttttac acctgaattt tttcctgcac
     7801 ctatgacaaa actctcatac cagtttccat catttttacc aacaacactt gctgaaatac
     7861 ttgtcgcctt atactcttct tgtacaaagt tcagtgtctt ttttaactct tctagcgatt
     7921 ctgttttatc tagtttggca tttaaagcta tcatattttt tttaagtttt tcattttctt
     7981 cttctaattt ttttaccttt ttagaattat ctttaaaatt tgctacatct ttaaaaccat
     8041 ttttgataaa agaaaatcca tcatttattc ccttacctac acttgtaata gcatctgaag
     8101 ctactcctaa atttactgga ctaccactag aaaatttacc aattgatata cccacaattc
     8161 caattaaagt gatagcaact acgcccgttg ctatcacttt aacattaatc ctttttttct
     8221 catttttgtt cttgtcaaat ctcaaggcca tcaccaactt ctaattctta gtattcatca
     8281 ttaatacttt ttcaaagatt tcttggtctt caacagattt acctgttcca agtgcaacac
     8341 aatcaagagg tgtttctgct atttgtacag acataccagt ttcctgtgta ataagcttat
     8401 caagacctct aagcaaagcg cctcctcctg ttagcattat tccgttttcc attatatctg
     8461 aggctaattc tggtggagtt ctttctagtg tagattttat tgcatctact attgaactta
     8521 caggttcttt taatgcttct ctaacttctg tagagcatat ttctattgtt ttaggtaatc
     8581 ctgagattaa atctctacct cttatttgca tatttatttc ttcatcatct ttaaatgtag
     8641 aacctatgtt aatttttaca ttttcagcag ttctttcacc tatcataaga ttatattctt
     8701 tttttacata agccactata gactcgtcaa attcgtctcc acctattctt atagatttag
     8761 cagtaacgat tcctcctaaa gatattacag ctatttctga agtacctcca ccaatatcaa
     8821 ctaccatatt accttctggt tcttcaactt ttaatccagc ccctatagcc gctgccattg
     8881 gctcttctat aaggaatgca tctttagctc ctgcttgtct tgcagcctct tctatagctc
     8941 tcttttctac ttctgtaact ccaaaaggaa cacatatagc tattcttgga cttacaacac
     9001 cttttttatc agccgctttt tgtataaaat agctaatcat tgattgagtt atgtcaaaat
     9061 cagctattac tccatctttc ataggtctta tagctactat atttcctggt gttctcccta
     9121 tcattttttt ggcttcttca cctactgcta atacctcttt actatcatct cttattgcga
     9181 caactgatgg ttctctaaca acaatacctt gtccttttat gtaaactaat gtatttgcag
     9241 tccctaaatc tattcccata tcctttgtca tcttattaaa actaaaaaaa cctttttttt
     9301 ctttcttttt ttccttagcc atgattttac tccttcctac tattaagaat catgtttaaa
     9361 acattctcct attattttat cacatataaa gtcaaattat catattttct ttaaagctaa
     9421 agtataatcc atctccaata attatatggt ctattatttc tattccaatt atttctccac
     9481 atttaataag tcttgatgtg atatttttat cttctcttga tggttctaca cttcctgaag
     9541 ggtgattatg catcactatt atcttacttg cacttcttct tatagcttct ttaaaaacct
     9601 ctcttggatg gactaatgac gagcttaaag tccctataga tacatctaca tcagatatta
     9661 tctcattttt tgtgtttaaa agaaccgctt taaaaacctc tttttttaaa taccttaggc
     9721 tatccatata atacctctgt atatcccaag gattccttat tttatacttt ataggtttaa
     9781 aactagctac tctagagcct agttctaaag ctgctttaat ttgggttgat tttgataacc
     9841 caattccatc tatattacac agctcctcta ttgtcatatc ttctaaatgt ctaataccct
     9901 gaatatctct atttattata tagttggcta attcaatagc atttttattc ttgttgccag
     9961 ttcttaaaag tatagccaac aactcagcat ttgataagct ttttacgcct ttggcaagca
    10021 tcttttctct gggtctttcc tctggtgcca tttcttttac cttaatggtt gaattaaatg
    10081 actttttcag ataaatcaca ctccataaaa aagatttata ctaaaatatt tttttaatag
    10141 gtcgcctaac cttgaaattg gaagaccaac tatattaaaa taatctcctc taatttcttc
    10201 aactaacaaa gctccataac cctgtatacc ataggctcct gctttgtcaa atgactcatt
    10261 agttttcaga taatcctcaa tatcctgctc tgataacgtt ttaaatttca cattgctgat
    10321 tacatagtca attacctttt tatcttcgca caagtttatt aagctaatac ctgttatgac
    10381 ttgatgctct cttccagata gtctttttaa catatctctt gcacaagact catcttttgg
    10441 ttttcctaaa atagcattgt ctaaaacaac aactgtatca gctccaataa caacatcttc
    10501 attatgttca gatgctacag acatactttt ttcaaaagct aatctcataa ctaaatgttt
    10561 tggtgtttca ccttccaata taatctcatc aatttcattt ttaattatat caaatcttac
    10621 attcgtattc tctagaattt ctttccttct tggagaggct gatgccaaga ttatattcat
    10681 ttattcacct catttgtacg taatttgtac ctatttagtc tatcactata ttgtaaaaaa
    10741 ttcaacagaa atgtcgaacc ttttttgtta cactattgtt atatttatta ccattagaat
    10801 aaatatacat aaaatagagg tatggaaatt attccatacc tctgttttat gtactcatta
    10861 gagatatgga aattattcca tatccttatt ttgtccatat tacaaaattt taatagcttt
    10921 ctttggacat ttttccatac ataaatggca tcctacacat ttatctgcat ctaccttatg
    10981 tttttctttt aattctccct ctattgcatc aaacttacat tgttttttgc acactgtaca
    11041 tccaacacaa agttcttggt caatagtaac tttctttttc ttagttatgt cacctgatat
    11101 tacttttgta gggcattttg ctacacattg catacaacct acacacttat tagggtctat
    11161 cttagctatt ttgtcttcaa atattattgc atcaaattta caagctttta cacacattcc
    11221 acagccaata catccagcag tacatttttc tttaacagct ttaccaaact ctttgctatt
    11281 acactctaca actacttctt gactttctgg ttttgtgatt attataccct taggacaaac
    11341 ttctttacat tttccacaat ttacacactt ttcttcatct attactgcta ttccatcaac
    11401 tatagaaata gcatcaaatt tacaagcatc tttacatgtt ccaagaccta aacatccaaa
    11461 tttacacatc ttagcacctg agtttaaaac atttgctgct ctacaatctg atattccttc
    11521 atactcatat ttatcttttg cacttgaaca agttccttta caaataactt ttgctacttg
    11581 tttctcacct gcttcagcac ttattcccat tatttctcca acttttgcac caacatctgc
    11641 tccacctaca gggcaaccat taactggtgc attgccttca actatcgcat ttgcaagacc
    11701 tccacaacct ggaaatccac aacctccaca gttggctcct ggaagtactc ctagtatagc
    11761 ttccactctc tcgtctactt ctacagcaaa ctttttagat gcaaaatcta gtactatccc
    11821 gaagattaag cccataattc ctaaaactaa tacagctgta agtatcacca ttttcacccc
    11881 tcttttttat agctttataa gtcctgtaaa tcctaagaaa gctattgaca ttaaacctgc
    11941 tgatataagc gttataggga aacctttaaa tgcttctggt atatcagcta attctagtct
    12001 ttctcttata cctgcgaata ttactattgc taaagtaaat ccagctccgg ctccaaatcc
    12061 gtttattatt gtttctacta aattataact tttttgaaca ttaactagag ctataccaag
    12121 aactgcacag tttgtagtta taagaggtaa ataaactcct aatgcttgat ataaagatgg
    12181 actcatcttt tgaattacca tttcaacgaa ctgaactata gaagctataa ccaatataaa
    12241 agctattgtt tgtaagaatc cagttccagt ttttactaat aaaatctgta taaaataagt
    12301 tataattgaa gctagagtta aaacgaacgt aactgcaact cccatcccta ctgctgtatc
    12361 aaccttctta gaaactccca agaaaggaca tatacccaag aattgagatg ttataacgtt
    12421 attaacaaga acgatactta aaaataaaag tattaaattc atttattaca cctccactat
    12481 gcttttttac ttcttaattt attaaatcct gcaaataaga atcctaatgt taagaatgca
    12541 cctggtggta atataaatat taatactggt tggaaagatg ctccaaataa tgtcattcca
    12601 aataaactac catttccaag taattctctt actgcaccta ttattgtaag tgcaacagta
    12661 aatccaagac cttgacctac accatcaact attgaaggca tagctcctgt tttgaaagca
    12721 aaactttctg cacgagctaa tattaaacag ttaacaacta ttaatggtat atatatacca
    12781 agagccttat caagagcagg aacatatgct tttaaaagca tacctactat tgtaacaaat
    12841 gtagctataa ctactataaa tataggtatt cttatcttat ctggtgtaat ttttcttatt
    12901 aaagatataa ctaaatttgc acatattaaa actgctgctg ttgaaagacc cataccaatt
    12961 ccatttattg ctgaagttgt aacggctagt gttggacaca tacctataac ttgaacaaat
    13021 gttgggttct catctattag cccattttta aatactttag ctaaattcat aattaaccct
    13081 ccttatttat taagagcact attgaaaaca tctatagcag catttactcc tgtagtaact
    13141 gctgtagatg taatagttgc acctgatatt gctaatattt gattatctgc tgttgtactt
    13201 ccttttgcta cttcaagagg ctcagcagtt tttcctttgt attgaccctt aaatgcatca
    13261 tccttagctt ttgctccaag acctgctgtt tctgacatat taccaatgtc aacacctgta
    13321 acttgacctt cagatgatat acctaccata acttcaattt ctccaccata tccacttggt
    13381 ttagctttta aagtatatcc aaccacttca gaaccatcta aaccttcata cacttcagct
    13441 ataagaccat ctccaaggcc tttgtaagta tcttccttca tttctttaaa ttcttttgct
    13501 tctggaagga ctgcttttct aagttcatta tttgcttgta tatttctttg ttctattact
    13561 ggagctgtaa cctgattagt agctccaaga gctaatgcag ctattgcact tatagcaagt
    13621 aaagtaccac ctaatctcac catactgttc attatttagc cacctcccca aatactctat
    13681 ttctaacaaa cttatctata agtggagcaa gacagttcac taatagtatt gagtaagata
    13741 caccttctgg atatccacca tacattctta taacagttgt tataagacca gctaatacag
    13801 cgtatattat ttgacctttt ttagtcatag gcgaagttgt ataatccgta agcatgaaga
    13861 atccacctaa cataagtcct ccagcaaaca gttgatatat tgcgaaagtt atattaaatc
    13921 cacctaaaag gaatgttaat ataaatacag ttgctatgta aaatactggt ataacataat
    13981 ttattatgcc tttatacatt aggtatactc cacctaataa taataatatt gctgatgtct
    14041 cacctataca tccaccaata ttccctatca tcatgtcagc aagactaatt ccattgctag
    14101 aaatatctgc cagtcctgct gctccatttt tcaaaaagct taatggagtt gctgtagtaa
    14161 ctgcatctaa atttttacct atccagttaa cacctggttg agtccaagca gtcattgcta
    14221 ctgggaatga tgctaataaa aatgctcttg cagcaagtgc tggattgaca aagttacatc
    14281 caattccacc aaataccatt ttaactacta atattgcaaa tattcctcct actagacaca
    14341 tccaccaagg aagtgatgct ggtacgttaa atgctaataa taatcctgtt acaacagctg
    14401 aaaaatctcc tatagtactt ttttgcttcg taaatttttg atataaaaat tcagtaccta
    14461 ctgtaccaag tatacaaaaa aacatagcac tcaaagcatt aagtctaaag aagtatactc
    14521 ctgctactgc tgctggaagg agtgctataa taacttgttt cattatatat gaagtatctt
    14581 cattacttct cacatgagga gaagatgata ctatcaactt attttccatc gtttttcccc
    14641 cttaactttc tttatttctg agcagcttgc tttcttctct ttgctcctat ttctctcttt
    14701 gctgatctta tagattgaag taatggtctt cttgctggac aaataaatga gcaagaccca
    14761 cattctatac aatccattgc tctatgatat tcagctgtgt catagtcatc ttttaaagaa
    14821 tatgcactta tgaaaagtgg ttgtaagaat gatggacata catctgtaca tcttccacat
    14881 cttaaacagt tttgaatatc tggagtacgt gattcttcct catccaaaca taaaattcct
    14941 gaagaacctt tgttagtcgc tatttcagta gtatattgag ctattcccat cataggtcca
    15001 cccattataa ccttaccaat cttcttatct tctttaaatc caccacattg ttctattatt
    15061 tctgaaacta atgttccaac ttttgttatt aggttctttg gctctttgat gcaactacct
    15121 gttattgtag tgattctttc aaccaaaggc atacctgttt taatagactt agctatctgt
    15181 gctgctgtgg caacgttgtc aacaactgca cctgctgcta ttggtaatcc accagatgga
    15241 acttctttgc ctgtacacgc ataaataagt tgtttctctg ctccttgtgg atatttcact
    15301 tgaaggccaa caacttcgat ttcgctatag tctttagcta cattttgaat tgcttctata
    15361 gcatctggtt tattcgtttc aattcctata aatccttttt tgacatctaa taccttcatt
    15421 aatgctctta aaccaaacac aacatcttct ggattttcaa ccattaatct gtggtctgca
    15481 gttaagtatg gttcacattc tgcaccattt aatataacta cttctgcctt gctatctggt
    15541 ggaggagata cttttacatg gttagggaat gtagctccac ccatacctac tattcctgcc
    15601 tcttttatga tacctactat atcttctttg ctcaaatcct ctaactgacc ttttggttga
    15661 acactctcat gtaattcttc cttaaagtca ttttcgataa ctacacattg agctgaacct
    15721 cctggtactt catgttgttc aatagcaata actttacctg atactgaaga atgaacgtta
    15781 catgaaacaa aaccttgttg ttcacctatt ttttgcccaa actttacttc atcaccaact
    15841 tcaacaatag gtttagctgg tgcccctata tgttgctgaa gaggaatgta aactatcttt
    15901 ggagcttgag ctttttcaag tgcctttgtg ttagagtact cttttctata tggaggatgt
    15961 atccctccct taaaagttaa gagtttcatt gttttacccc cttatctata aaaaaacatt
    16021 ttttctgcct tctataagta tactatatat attaaaaaat cttaacaatt gttaaaaaaa
    16081 tatccgtatt tttataattt attttataaa tcaacacctc tatattaagt caaattgagg
    16141 aaaaggattg cttaattaaa caatcctttt catatagctt tctaaagtcc aaattttctg
    16201 tattttattt tatagaattt ataaacatag agtactcctg catggatgat aaaagcagac
    16261 ttttacttac actatatttt tcttcatttg ctgcttttga agctgtatca attttttcat
    16321 tcacattctt tacatactct attaaattat ctttaaataa acgcatttca gaataatcaa
    16381 tcttttcagc ttcttttgat atattttgtg atattgcctt tctatttgtc aaaatagtat
    16441 tatatttttc catatctaca ttattttcag cattcgacca aaaacttgat tcttctttaa
    16501 agtttgttat taatttattt aattctttag atatactctc tatataagcg tagttacttg
    16561 tatactctaa agacaacata ggtgcatcta agtgtgataa aaatgcatct ggaaacacct
    16621 ttcttacact gcttatctgc tttctaacat cattttcatc aaaagaggaa taggtctgta
    16681 ttttttttac tccatccttc tccataactg aaaaaggtat gtcattatta actaatgatg
    16741 tctgtatttt tttcaaatca tcattattat ctattgcagc aacttgaata gtatacatat
    16801 cccagccttc tatagtagct aatttgactt cttctggctc tttagtgtta tttaccgctt
    16861 tttctggatt agtttcttgt ttatcatctg aattggtttg tgatttagat tttttccctt
    16921 tttttattga atttaattca tcagatatat ctttataagt tatatctgat gaattattta
    16981 gaaatggtat tttagccgat acatactcca atatcttgct atcttttatt tttgtataag
    17041 aataatagcc aattagacac aagcaagcaa tcgttataac atatttacgt aaatcaatct
    17101 tatgtctatt attattaaat cctctgtata tcgtttttct tctattcatt agtttgtccc
    17161 tcccaaaatt atattattaa taaagtatat ttttaagagt cacaatttat gaatataaat
    17221 aattatttct tctaattttt taataagaaa ttgtacttac taaacaaatc ttcttgcacc
    17281 aagtaatact ttagaccaat atgaactagt taaattatcc actttaacct tattaggatt
    17341 agtctgagta ccactacaat ggataaattt actatttcct acgtatatac ctacatgatt
    17401 agttgttcca tcaccatcag tgtcaaaata aactaaatct cctggtgcat aatttcccat
    17461 agaaattgca cttccaaaat tagcctgttc tctagaaact ctaggaatag aaacgcctac
    17521 agaatgttta aaaacatact gtgtaaatcc agaacagtca aatttatcag gtcctgaagc
    17581 tccccattga tatggtattc caataagagt atatgcaaaa tctacgattg ttgaaccttt
    17641 tgctgtagat ttatctgaat ttccagtaaa tgatggcttt gagttactat tagattgaga
    17701 attttggtta gatgatgaat ttggtttttc ttttgtatcc tcattagtct cagaaatata
    17761 actggcactc acccatccaa ttgttccact cgataacttt accttatacc aaccattact
    17821 tttagctttt aattcaacaa catctccacc atttaatttt cctattatag aatagcttgt
    17881 cccaggacca cttcttacat ttaaactcac agcagatgtt accttaccat tcttgccttc
    17941 aattgattct tcgctagatg gttttttatc agaattatta gtactatttg aattactaga
    18001 attgttcgtt ccaacatctt ctgatgtttt agaaatatat tggctactag cccatccagt
    18061 aactccattt gatagtttta ttttatacca tccattgctt tgttctagta actttactac
    18121 atctccatta tttgctttac caactaatga atagttagta cctgcaccac ttcttacatt
    18181 cagtctagaa ctcacattta ctttaccatt tccactaata gttgtcccac ttgaagttgg
    18241 attattttga cttgatgtac cttcagacga accacttgtt gatatgtatt ttgcacttcc
    18301 ccatccaaca acagaactcg atacccttac tttataccat ccattagact tttctaaaat
    18361 ctctacagtt tttcccttgt ataattttgc taccttatcg caatcagaac ttggtccact
    18421 tcttatattc aaggcacttg ctgttactgt tcccttttca agagcacttg catttatgga
    18481 gctaacagat actgctactg ccccaattcc taaagctgct atagcttttt tcactactat
    18541 cactcccccc aacaaatttt tatagtcata aattcataac attattatcc atttttagtc
    18601 cctacaattt aatgataact tattttctta ttaattgtaa agaaattata aacatatcct
    18661 taaaattagt gatttttagg aagtatttca atccaataat tatctgggtc cgctataaaa
    18721 taaattccca tactttcatt ttcaaaacat atgcaatcta aatctttatg aagttttttt
    18781 gcctcatcaa actcctcaac ttctaaagcc agatgaaatt cattgtctcc taaattatat
    18841 ggtctatccc aatctctaag ccaagttaat tctaatgtat gactagtaat gccatctcct
    18901 aaataaacta atataaaact tccatcttct gcttccttgc gctttacctc tttaagccct
    18961 aaagcttttt tataaaaatc taaacttttt tctaaattag taacattgaa attgttatga
    19021 caaaaattaa acttcatttt gaaatcactc tccaattttt atttttttta tattatatca
    19081 ttattataaa tttatttaaa tcatattaag ttaaaaattt gaatgatgat gaccaattac
    19141 caaatcatgg taaattattt aaaagaatat gtcaaaaaaa gaataagctc tttcaaatgc
    19201 ataaaaaatt tatacccaag ttatacttaa tcatgcaaat tggcagaact tattctttaa
    19261 aactctaact tttaagtttt tctttaataa tataatttat tacttttttt ctaatatctt
    19321 cagcattata atcatcttct acttctatat ccaaaagttt tgctattgac ctcatatctt
    19381 ctctagtctt tgacctatag cttgatatta attcatcttt tattttctct ggttctaaaa
    19441 gatattcttc atctcctctt ttgaaaacaa cgtatttttg agcatatgaa cttctattgt
    19501 agtatatata tcttgatatt tttcttaaaa tttgattata tgaaagtcgc ttgttaatat
    19561 caagttttaa gtactctgaa aaagcaaata gctctttttt tgatttaaat aatgcttctt
    19621 tctttatagt tcttgatatt gaagttacac tttcttgata tgcacacttt tttaacattt
    19681 ttaaatatac tctattaaga ccaatacttt gattgaattt ttttatatta ttattaataa
    19741 tcttatccaa gttaccttca aagtattttt ttgctccttt ttgtgataca taactctcaa
    19801 gttctttgtg ggccatatca agtatcattt tttttaaatc ctctttatat cccctagatt
    19861 ccatgatact ccctccctta gctatttata gtatattcaa aaagctcaag aaatgtgagt
    19921 cattacaaaa tttttttact ttttatgtat atattaattt aaaaggggag ataatagtaa
    19981 tgtaaaatca aacatactca atctccccca tttttagacc tctattactc ccccaataga
    20041 ggtctttttt tagtttatca atttctattc aattcccttt agttcaaatc ctaattctgc
    20101 tatcacttct tttaattttt caatagatac atcatctttc atatctacag atgcacactt
    20161 attttctaaa tctacatcta aaacttcaat accatctata tcttctgtta atgcagtttt
    20221 aagatggttt acacaatgac cacaactcat accttctact aatagtttct tcttcattta
    20281 attacctcct tataccctat aagggtattc tatcatatag tttatacttt ttaaatagaa
    20341 attaatcatt taatttgatt cattcgattt tatatctaaa ttagatattc cctcatatac
    20401 atcaaaatca tttggagtaa ttttaccttt taatctacta ttacttacaa tatagttgtt
    20461 tctcacagct aaacttataa atgttgcatt atcttttata tatgtctgta catctttgta
    20521 tgcttttttt gtttcaattt cagacgtaga acttgtaagt gcagacatat atccatttaa
    20581 cttatcatca gtatatccac tactcgctat aatactactt gcatctggta taattgatag
    20641 tttccatcca actaatgcta aatcataatt tttagaatta agagcatttt ccatatcctt
    20701 atctgacagc tgatttactg tactatttat tcctatagct tttaagtttt catttattat
    20761 gtttgcagtt tttattctct cactattatc tctgtttact attatcttta agttcatttc
    20821 agaaagactt ttctttactt cctctttttc tctatttttt ttctcttcag cttctttttt
    20881 gattctatct tcttctgctt ttttagcttt tgcttcttct tctggcgtaa gcttctttga
    20941 gttcttttta gctgattttt tctgattttt tactttattg tcattttgat ttactgagtt
    21001 tacatttgca tattctacct tagcaagaca ctctatcgcc ttatctttat taaactctaa
    21061 atccttcatt tcactattat agtattttga ttttgaatta agtggaaaat taactggagt
    21121 agcatcatcc atataacctt cactaataat tctatcttta tctatagagt gtgcaattgc
    21181 ttttctaaaa ttaacatctt tgaaaaaagg attattataa ttaaataata cacactcata
    21241 atctcttcct tgatattttg ttatattaaa ctctttttct tgaaacttac ttaaatcatt
    21301 taatgtaaca ttggttatat cactatcaag agccataacc atagatgtcc ttgcatcttc
    21361 attaggcacc atacctactt ttatgttctt catggttttt ggaacttctt catagtaatc
    21421 tttgtttaca gataaaacca ttccttctct ttctgtatat gattctattt tatatttccc
    21481 atttcctatc atattattat tcttgtcatt tacatctttt gaatctaaat gattttgaga
    21541 tacaattgga aaaattaaac tatcaattga aaaagcatat ctagctttaa attttattgt
    21601 aaaatcttta tctgatttaa tattcacaga agctatattt ttagtaaatt cattatatgg
    21661 actatctgca cttttttgaa ttaaaccaat agtatagttt acatcatttg aagtaatatt
    21721 ttttccatca tgccactttg catcttttag ctttatatct atactcattc catcctgagc
    21781 tatattatac tccttaacta attgaggaat tacattgtag ttctcatcta tagtaaataa
    21841 actatcataa actaaattca ttatataacc aacagactta tcagtattag ttataggatt
    21901 tatagttttt ggagttacca ttgttaagtt tatatactta gcatctatag aactttgact
    21961 ttgtaaactt gaatcactac tactttgttt tgttttagta ttactacaac cagctaccat
    22021 aagagttata gctaaaacga ctgttaagac ttttattctc ttcattagtt ttttcctttc
    22081 ttgccatata tcttattata atgttcgtaa ttatttttta ctttttctac atattttgaa
    22141 gtttgcttaa aaggtatatc atgtagattt tcgccatctt tactatattc attattttct
    22201 aaccattttt tcacattacc tgaaccacca ttatatgcag ctatcactaa atctaattta
    22261 ccaaattctt tgtataactt acttaaatac caacagccaa gtcttatatt agtctctggg
    22321 tcgaaaatat ccacattttt taaatttagt tcctctgctc cccaatctct agttatgtct
    22381 aatatttgca ttaatccttt tgcttccttt tttgaaacag cagaactatt aaacttactt
    22441 tcggctttaa taacactgta aactatattt tcatctaaat taaattcttt cgaatacttt
    22501 tctacatact ctgaatattt tttaggatat aaaaatttat gtattacttt gctttccatt
    22561 aatagtgccc caaataagat tataaaaata gatagaatca atactttctt actattcacg
    22621 ccaattctcc ttcatgtact caataaattt atgtgcttta ctttctaatt cagttattgt
    22681 tcccgaatta tctattatat aatcaccata tttggatttt tcttcctgac tcatctgtga
    22741 atttatccta ctaagtgctt cctgctcact gcaattatct ctcttttgta ttctactaat
    22801 ttgtacttct tgtttacaag taactactaa tagcttgtca accatatcta aaaaaccact
    22861 ttctaccaat attgccgcat ctaacactac tatattttcg cctttttttc tcaatttttc
    22921 tatttcactt attatttttt ctcttattcg tggatgagtc aagttattta attccttaag
    22981 tttttcttca tcactaaaaa ctattttccc aagagctttt ctatttagag ttccatcatc
    23041 attttttata ctttgcccaa aatggacaaa tactttttcc aataacaact tatcctcaaa
    23101 tatcttcctg gatataatat cagcatccac tataggaatg ttaagatttc taaatatatt
    23161 tgataggctg ctttttccac agcctattcc tcctgtaagt cctaatatca acataaaatc
    23221 acctactttg tctcatacca agaactacct acatttaaat ctacatctaa gtgaacatcc
    23281 atatttacag cattttccat ttcttctttt actattttct taactatatc tatttcatca
    23341 tcaattgctt caactatcaa ctcgtcgtga acttgtagta taagttttga tttcaaattg
    23401 ttctcttcca attttttatg aacatttatc atagcaattt ttattatatc tgctgcactt
    23461 ccttgtattg gtgcattcat tgcaaatctc tttcctctat ttctttccat aaaattgctt
    23521 gactttattt ctggtatata tcttcttctg tttaaaattg ttacagcata tccatcctca
    23581 ctagcctttt ctactgttgt gtccataaac tcttttattt tatggtattt agcaaaataa
    23641 ctttcgatat attctttcgc ctttggaaca ggtatattta aatcatcagc tagaccaaag
    23701 tcacttttac catatataat accaaagttt acagctttag ctgcacctct caattcacta
    23761 gtaacatctt ccataggaac attaaaaact tgtgaagctg ttttggtatg aatatcttca
    23821 tgatgtttaa atgcttctat catattttca tcctgactca tatgtgcaag aactcttagc
    23881 tctacttgtg agtaatcggc atcaaccaac ttacagttat catctgcaat aaacactttt
    23941 cttatttttc tacccatttc catttttata ggtatatttt gaagatttgg ttctgtagat
    24001 gaaattcttc ctgtagtcgt tatagtttga ttaaatgatg aatgtattct attactttct
    24061 ggatttatta tatttaaaag tccttcaacg taagtagaat ttaactttac tatttgtcta
    24121 tactcagtta tcttatctat tattggatgc ttatctctta atttttctaa aacatctgca
    24181 ttagttgaat atccagtttt agttttttta attattggaa gctctaattt ttcaaataat
    24241 ataacaccca attgttttgg tgaatttata ttgaatggct ctcctgataa ttcataaatt
    24301 tccttttcta atgtagctat tatttcttta aattcaacac tcaattcatc caatatactt
    24361 ttgtctactt ttataccttc atactccata tgacctaaga cttctactaa aggcatctct
    24421 acatgataaa aaagaccatc catactcata tccattagac ttctttccat cataggaact
    24481 gtttctttaa ctgtgcatat tatttgaccc atatactctg ctaaatcctc aaattctaaa
    24541 tcctcatagt ttttagattt aacacccttt cctaataatt cttcttttga tttaaccttt
    24601 tttgataaat acttcatagc tattgcacta caatcatatg aggtagatga agttgaatct
    24661 attaagtact cagctattgt tatatcaaaa tacatatttt ctaagtttat attatatggt
    24721 tttaaagata tataatcatc ttttaaatta tatccatact ttttaatttc attatttgaa
    24781 aatatactat ctaactttga cacttcattt gaatctacat aatatatttt ttctccatct
    24841 acacttataa aaatatatat tatatttttt tcaagtatat taccttgtcc tctaacagtt
    24901 tttaaaataa cttgtccttt tttgtttact tcatctataa atttttctat atcttttaat
    24961 tttaatattt ctattttttc ttctacgtgt tctgtgtcat ctgaatcgac caaatcaact
    25021 aatcttgata agagacttgt aaatccaaag tatctaaatt tatctaaaag ttcatcttta
    25081 tcataatcgc caaaaatcat actatctaaa tccacttcta ttggcacatc tcttataatt
    25141 gttgcaagtc ttttactttg aatagctatt tctttgtttt cctctatttt cttttttaca
    25201 ctacctttaa gcttatctgt attctctatt agattctcta tactagaaaa ctcttttata
    25261 agctttatac ctgttttttc acctattcca ggtactccag gtatattatc agacttgtct
    25321 cccataagac cctttaaatc gataaattgt gttggagtca tctcatattt ttcaattaca
    25381 gaattaaagt tgtattcttc tacttctcca accccttttt tcgttataag ggtagttgta
    25441 ttatctgaag caagttgaat agcatcttta tctccagtta ctatgtatac cttatatcca
    25501 ttttcttctg cttttttaga aacagtacct ataatatcat ctgcctcata accatctatt
    25561 tctagtctat ttattctaaa tttatctaaa aggtctttta atggctgtaa ttgttctgcc
    25621 aattcatctg gcataccctt tcttccagcc ttataatctt tatattctaa atgcctaaat
    25681 gtaggtgctt ttctgtcaaa agctacactt atatgtgttg gtttatatat atctatcatt
    25741 ttaaatagca ttgtagtaaa cccatatata gcattggtct ttaacccttc tttattattc
    25801 atttctggaa gagcataaaa agctctattt attattgaat ttccatctat tataattaat
    25861 tttttttcca aattatatca ctcctaaaaa ttatctattt ttgtaacata tatattatat
    25921 accaatatag tgttatttaa tattcccata ataataatag tatattaaaa ttaaatatgt
    25981 tgcaaacaat atattttttt gctataataa tatacatgac atgcccgagt ggtggaattg
    26041 gtagacgcag tggactcaaa atccaccgga ctaatactcc gtgccggttc gagtccggcc
    26101 tcgggcacca ttaaaaactc ctaaagctat ttattcaata gattaggagt ttttttattt
    26161 ttgatttaaa ttttaagtta ttttttgaaa ttactgtgtc tttttaaaat ttatataatt
    26221 atataaattt atcttctagc attaatttaa aaactaattt acttttatat aattttctat
    26281 tttatatcat aacttattac aaccttttat ctaatttatt gtatacaaca acactacaaa
    26341 taaattgaaa taaaataagc aatccaagaa caactaaaac ccacttgctt attaccatta
    26401 cagaaaatac actcaatata gaaacaataa atataagaat tacaagaaca tagcacatga
    26461 tttgattagt aatacgtcca gctttatcac gtaacataat tcttctttca tcattcatct
    26521 ctatcctttg attttttatc ttttcttcat aatctactgc tctttcaggt ttactccaat
    26581 acatatattg aagaatcata catacacctg gacctaaagc agctccagat aatccccaaa
    26641 taaaagcctc aaatgaaaac tcagtaaatg tagccaataa aacaagtaaa attccacaaa
    26701 taaaatatat aattccaaca tataacatat tctttttcat ttttactcct cctcataaat
    26761 aaatatttct tcaatactca tatcaaagta tctagctatc ttaaaggcta agatgataga
    26821 aggattatac ctaccatttt ctaatgaacc tatagtttgt cttgatactt gcaaaataga
    26881 agcaagttct tcttgtttta tgcctctttc ttttcttatt tcttctaatc tatttttcac
    26941 atctatcccc ctttaatatc tcaactacgg aaagtctact ttccataatt atacaactat
    27001 attccattta tgtaaagctt actttccata tttacaaaaa aataacagac tattttcagc
    27061 ctgtaatttt agaatttcat gttgtttatt aattttaatg ttatttatta attccatatt
    27121 atttattaat tttaacttgg tttctgaatt ggaatccaaa cttctgtagt ataatcagga
    27181 gcattgatat cgccattggg ataaagttca atatctggta aattagcata cacataccca
    27241 gaacttggta accactcagt aataattctt ttttgcaatt cctgtattgc cagagcattt
    27301 ggcattggag aaacacattc aaatactgcc catgtgcctg atggtattaa gtattcatga
    27361 gtatcttcta atacaggctt atctgttgga gcagcaatat aataatctaa acccttacta
    27421 tttgtacaca cactaactcc aagcaatcca caaggttcat tatttattaa ctctgaaagt
    27481 ttatcaattg ttccattttt tatgcattta gcccacaact taggaacttt ctcaaagcac
    27541 tcttctacaa tcatgttcat actttctttt ataccaacaa tacgaaaccc tttcttttct
    27601 acaatacgat attgcatttc tatatttcct tttacataaa tgcttaatgt catcttagga
    27661 tatgctttta aaacaacacc ctttgctcta gctgcagatg gagaaatgtt atgtatattt
    27721 tgaaaagctc tattaaaaga tgttggtgaa tcgtaaccat acttctcaga tagttctaaa
    27781 attttgatgt tactgttctg aagttcaaat gcagccttag tcatacgtct tcgtctaata
    27841 tattcagata atggtacttc tgcaatatat gaaaacattc tttgaaaatg aaaggttgaa
    27901 caacatgcaa tttttgctgc ttctgcataa tccactccac tttcgagatt ttcttcaata
    27961 taagtaatag cttgatttaa tttatttatc cattccattt gatattacct ccaagcaaat
    28021 tatacagttt aataaaataa tatacctctc tattcatgca caaaattgta agcatttaaa
    28081 tttataagca tttttcaatc aatacaataa atataaaaat tgtattatac aataaaaaag
    28141 ttcaataaca ttatattgta aaatgttatt gaacttttta taaaaattaa tttaagtgta
    28201 gttaaattaa gtattacact atcttatata ttacaatatc ctactatctt atagtcttct
    28261 ggcactttat aatctttgtc tggagtacct aaattccatt ttaaatcaaa catagttgca
    28321 cttattattg ttgcaaaata aagcataact ataccaatgt ctattttttc ttcatataaa
    28381 ttagtatgac catcttttct aacagctaaa actacagtgc ctccatcaac tataaatctc
    28441 catggttgtc tatttaatgt agatggagct aatcttgcat agtgaaatgc atcaagaagt
    28501 gctctttctt ctaagatttc tatacttgca acatttcccc attcgttcat ataaacaaca
    28561 tcttctacac ctagtctagt agacccctta tctgttacaa cttgcatatc agattttgaa
    28621 taattttgcc cagtttttgt agcatttaat actttagcat tacttacatt ttctccatat
    28681 ccaatagcta taattcctgt tacctcttta tctgaattaa ttccaagttt ttcttttacc
    28741 ttttttccat cttcaaaagt aacccaacaa gaaccaattc caagttctgt tactttaaag
    28801 attatacttt ctcctatgta ccctgaattt tcaatgtaat tatcttttat gtcagataat
    28861 attataatat aacttggaga atctatcata tttcctttgt accctgctac tttatctaaa
    28921 ttattaaaaa cttcacttct attcataatt cttacatcaa catctatttc tggaactagt
    28981 ttcttacaag attctgagta tttcttaatt tcactaagat attcttcttt aatatctgtg
    29041 ttcttatatt ctctaacaga ctttctattt aatatgattg ttttataatc cataatttta
    29101 tcctccccaa actagtattg tcttaattat atacccatta atatattatt ttactcattt
    29161 atttaataaa taaagattac aattttgtaa ttattattta ttaataatat tccactttta
    29221 cattaataat gaccagttgt gcaaaatcca ttgccaacca ccaaataaaa acatattata
    29281 aaggtaatat aaaataacaa acaaatctta acaaaaaatt aaactttttt tacacgaaag
    29341 aaggcttccc gttgccttct ttatttttat aaaaaaataa gctgtttcta aaatgatttc
    29401 acatttttga aacagccaag ttattctata atgacaacta taatttgagg atgtgaggga
    29461 gagttgtcat tataaattta gggtaattat attatgtttt aagctagttc tttctatatt
    29521 tatataatac attttatata tgaattttat atgagattgg tataaatgat atatgaaatt
    29581 acatatgttt atattatatt aaattttatt attacatttt ccacaacaat actcatatgt
    29641 ctccacatta tttggaatat ttacaaatac aaattcttta cagttctcac aatactttaa
    29701 tatagatata gttccacaag tgtaacattc aaatattaca caatcctcat tttcctctac
    29761 tatttcaaac tttttactat gttcaccaca ctctgggcac ttgtgattat ttacattcat
    29821 aaatactcct ccttattagt atagttacat gtcaaaaact taattaatgt attctatatt
    29881 acttattata tattctataa taaatcatat tctaatataa taaaacaaat taaataaaaa
    29941 aaaaaccata aattttaaat ttatggatta attcttgtaa tgataactct atttgaggat
    30001 gtgagggagg gttatcatta caatagggta ttatattatg gtttttaagc tagtctttct
    30061 acacctcata tgatacatat gctatatgaa ttttatatga tttttacata aatattttat
    30121 gaaatttaaa ttttttagtc attttgaaat ttgtaaaaag ttctctaaaa atccacagca
    30181 tcataaacag ccaaaattta agtaaaacat cataaaaaaa tttgtccaat aattttaaca
    30241 agtaatctca cctcattata attattgaca caaacttaaa taaacaatct ataaatcttc
    30301 tataaatctt aaactttccc cctattgata cacaatttct agtttgtcaa ttttattttg
    30361 catttctaag aaaatttctt caacagaatt gttaaatata ttgcctaaat agattctttt
    30421 atctttttga aatgaataag attttacact accatcagaa gaaatatata aatgactcca
    30481 ctctgctcca tattttgaaa acctctcctt tgtatcttta aacctcatat cttctatttt
    30541 tattgctttt tctctgtctc ctcttactac agtgtgaccc ctttttatta aactggttat
    30601 ttttatatct tcttttaatt taatacgtgt atcatcattt tttgaatata ctttccatat
    30661 cactttatga cgataattat ctaattcctt tattaagctt ctatcaatag taaaactatc
    30721 tttagttacc acaattaaat caaaatggtc taacatatat tgaaatattt ctctcatact
    30781 tttatttttt aaaatatcct caccaataac ctctataaat ctacagcctt tctcatacat
    30841 gcagttcaat aaacttgtga tttcagaaaa ataatcattt gtattattat ctccatctat
    30901 ttctatagta aagtacaaag gagtaatgta tgatttattt cctgttatct caaatacact
    30961 tgatagtgtc tgtatatcat cttcaaacgt taagtacaca ttctcataaa acataaaaaa
    31021 ttccataact ttttctttca tattatggat atttctatat ctattggata atatatttat
    31081 tatatcgtca gtagtatttt tcccatcaca ttcagccagt ataacaattt gcttccaact
    31141 taagctatcc acatttccga tacttttttt atatccatct tcttcgtcat aaataggacc
    31201 aattataatg ttttcttttg gccacagatg aaccctagtt ccatctttta atttcggata
    31261 tttatttttt acagactcat aaaatctact tgtatcaccc atgattcttt actacccccc
    31321 atagtttaaa tattctatgc taaaaatagc actttatttt tattaattca acaattattc
    31381 aaaattggtc attatatatg tttaaatgga tttaacatca accttattta tatgttattg
    31441 atgtttatat atgttatata aaatcttatt tctcatactt aaatgtaatc ttaactatct
    31501 ctggtttaca attaagccta attaaaactt ttgagcctcc taagccttta tttaaaatca
    31561 tagtgctatt atctttttca tattttcccc agtcatactt aggaaagaac tctctatttg
    31621 gagataatac acctccaaca aaaggtattc ttattatacc tccatgaaca tgtcctgcta
    31681 aaaccaaatc tgctccccat tttgcatatt tttcaaagaa aaatggtgta tgtgctaaaa
    31741 gaatgttata ttcattttca ttaactttac ctagattgtc ttctacaaaa ttgtcactta
    31801 gttctaattt tttgttatta gcaaataaat atggatagca ttccaatggt ataattaaac
    31861 catataaatt aagatggcta ttacctctat atattcttac agattcattt tccatattta
    31921 ttattttctt tgaatatagt tccttaaaat aatccttata tagatgttta tactttttta
    31981 ttaaagattt ttgttcatga tttccaatta tatgatatac ttcatatttt tcaaccaaat
    32041 tatctattaa atctaatgca acttgaaaat ttttattttc tccatctact aaatctcctg
    32101 ttataaatat aacatctggg tttaatgatt caattttatc tatcaatacc ttattatttt
    32161 tatcaaacac cttattatgt aaatcactca cttgaactat tttaaagtta tcaaattcct
    32221 ttggaatttt tttgttgaaa atagtatatt ctgtcgtttc tatatgctga gtttcatact
    32281 tatacagaat atacaataat attaatacta atactgtcat tgaaaccttt ataatcatat
    32341 tctcttcctc tttattctat tttgttaata ttattataat gcttttatgt tttttttaaa
    32401 acaataacat catcacatac atataaatct tttcgacatt ataaaaataa gttatccttt
    32461 tctaaaattt tcatttattt tagaaaagga taactataaa attatctata aacctctata
    32521 ttgtgaaata tagtttttcc tttatttaaa tttttatata tatgctctaa agttccatca
    32581 aacctaatag aatgtccaga ctctaaaata taagttttcc catccacatt aaggtcaagt
    32641 ttaccttcta ttacgagcac atattcttct aaaacatgac tatgtgtaga tgaaacatga
    32701 gtacagtttt cctcaagctc aattataaaa agttcaaacc ttctttgagg gctaaaaggg
    32761 tatattggat ataacttcat gttccctttt tgctcaacaa taggctctac ttcttctttc
    32821 tttataacct cataggtatt tgtattttca tttaatagtg tagtaaaatt tattttaaat
    32881 ccagatgaaa ttttccataa agtagatact gtcggacttg attcacctct ctcaatctgt
    32941 ccaagcattg ctttactgac acctgtcatc tttgaagctt tgtctaaact tatgcccatt
    33001 tcttgtctca ataaccttaa attttctccc acattaggaa atttattatc catgttaaaa
    33061 tcctcctgat agctatttgt attctatgat ttttactcct gtaagttcta ctatctcttt
    33121 taattttaac atatgattgt tacaaatatc atcaaattca tatatataat cttcaagccc
    33181 taatgcctta tatttatcga ttccaaattt atgatatgga agtaactcca tcttgcctcc
    33241 tggaacattt tgtttgacaa ataatgcagt atttcttata ttttcttcac tatcatttac
    33301 tccataaata attggcactc ttataaccat agatttattc aactttgaaa gcctacatat
    33361 attatctaat ataattttat tacttactcc tgtatattct ttatgaatat tatcatccat
    33421 agattttata tctacaaata tgtggtctat tttctcaaat acatcattta ccttattcca
    33481 atcaaaatat ccacaagttt caattgcaat atttatgcct ttatcataaa aaatatcaac
    33541 taattctctt aaaaaatcaa tctgaagtgt aggttcacct ccagaaaaag ttatccctcc
    33601 attagattca aagaaaaata taaaatcttt ttcaacttcc tctactattt cttcaacact
    33661 catttcttcg gtcattatac aaatggcatc tttaagacat accttgatac actctccaca
    33721 taaatcacat ttattattta tttgcgctct atcaaatagt gaacttatat tttgaggaca
    33781 aacatctata catctattac aggatacaca tttatctttc ataacaccta attttactat
    33841 gttactccag gaatctggat tagaacacca cttacatttt aatttacatc cttctaaaaa
    33901 tatagtagtc cttattccat ctccatcatt tactgaaaaa tgttgtactt ttacaactct
    33961 acctttttta ctcataatct acctcataat taaaaattgc tatgtgaatt tctagctatt
    34021 atagcatctt gcatttctct tgataaatta acaaattgag tactatatcc agcaaccctc
    34081 actaataaat ctttatattc ttctggattt tcttgagctt ttaatagagt atcatttgat
    34141 atcgtattaa attgtacatg gaatcctcct aaagcaaaat atgatttaat catagatgtt
    34201 agatttctaa gtcctctttc agtttccacc aaatcttgat tcagtcttaa atttagaagt
    34261 gttccaccag tatgaacatc atgatttatt tttgatgcag atttcattgc tgctagtgga
    34321 cttgttgtat cagtaccaac aaatggagaa actccctctg tcaaaggttt tgtcgctttt
    34381 cttccacaag gagttgcacc tactattttt ccagttggta catagtttga tatacccata
    34441 aatgcactat taaatttaga gccaaaaata tccttgtatt ttctagtttc agtataataa
    34501 aaatcagaaa cttcaattgc taatgaatct acataatcat tatcatttcc atacttagga
    34561 acatccaacg caagttttct taattcttca taaccttccc aatcattatt tagtgctttt
    34621 gttaattctt ctaacgtagt caccttatcc tcaaatacta atttttttat agctgccaat
    34681 gaatttgaaa ctacacctaa ccctatacca gttaatacag gacctatatt atattttgct
    34741 cctcctttac ttaaatcaac tcccttatcc aaacatccat ctacacaagt agataaaaat
    34801 ggtcttggta ccatttcttt atgaatttgt tgagcaacag tagttcctat aacagaactt
    34861 tttacaagat atgctaattg ctttaaaaac tcatccttaa gttcttcaaa agaagtaaat
    34921 tctgtgatat tttttgtatc taaccccatt ttttctccag ttaatctact cttaccttca
    34981 ttcatagcat attctaaagc tgctgcaaaa ttgatattta cggctgaagt ccactgtcct
    35041 gtttttctaa aatgaggaac tacacaacca cagttactcc aatctctagc atcttctgca
    35101 tcatatccat cttgtaataa catttgtgcc cctgcactat cgctgtgtat agcagggaaa
    35161 ccagttcctt gttttaccaa tttagcaact gccattacga aattatctgg tgctccttga
    35221 ctcactctaa cacttaatcc tggttggtga gtttttacac tttctgtagc ctttaaacac
    35281 atatatgaga tatcattcgt tccatcagta ccatttctct ttttaccacc aacagtaaga
    35341 ttttggaatt gattatatcc tgcaaagtaa tttgctgtat ttgctgatat agtccatacc
    35401 cattctgata acttaatcca aagtgcttct atcaattctt gagcttctga ttcagttatt
    35461 ttaccttctc tagcatcatt ttcataatat ggatacatat attggtcaaa tctacctaaa
    35521 tttaatgcca atggattttc tgatagaata ccacctaatt gaacaaacca tacaaattgt
    35581 attgcttcat aaaaattagt tggagggttt gctggtacat tcatacatat ctctgaaatt
    35641 tttataagtt ctaactttct cttttcatct gtttcttgct tactcatctc ttttgcaaga
    35701 tttgaatatc tttgtgccaa tgtcataata ccctgtgcaa ctatagaaac agatttgtaa
    35761 aaatctatct tttcaatatt ttctgaaact gatatatcta attctttaat ttttttatct
    35821 gcatcttcct taatcccttt atatcctttt acaaacaata tatcttgata atctggtgtt
    35881 acttcaccaa ctgcctgtct ccacttagaa tcattatcta ttattcctgt atctactaat
    35941 atcttagctg tatcttctgg gattcttgct aagaaaactt cttccaaaga ttttcccttc
    36001 caatatggaa atatttcttt tcttataaat tctatttgtt cttttgatat tacatatggg
    36061 tcttgagttc ttttatcaaa agtatccatt tctttgtcta cccattgcca agaaaactct
    36121 ggtgtcaata tccctgttct tctaaatttt cctgctgtac ctactattaa ttcattttca
    36181 aagattttta taggaagttc tttacatgtc tccaaaaaag ctttagctct tcttatacag
    36241 atagcttctc cctcagtttt tttatgtgat tctgtaaaaa ttctagccct ttcacaacat
    36301 atcataggtt tagaattaat atattcttgt ctaaatgctt ctattctatt agtttgactc
    36361 atttaaaatc cccctttgga taatatatac tacatttttg tatgttataa catactttaa
    36421 ttatattata ctgtccttta gcataaaatg gaagcaaaat aacttatttt ttttcatcta
    36481 ccttgaacac ctatatatta atactttttt tgttaaattt ataaactcta aaaaaatttt
    36541 ctatgatata ttttctatat tgttatagtt atttctaata tactaatgat ttgaaatatc
    36601 aaatttgtat actagactta gttttaaaac cttagttgat tgtttaactt tgttaacttc
    36661 aacatgtata tccttcatat aaggaagctc tacaacacct actcccttat taaagtcttc
    36721 ttcaaccata gaaaaaattt ctttctcagt taagttcgta ttagtcaatg acttaaataa
    36781 attgtatata aatttaatat atttatcact ctcagataac ttatcatctt ctggatactc
    36841 tttagcacag agaatatcta ttgttacttt tccttcttta tcgtcttcag acatagaata
    36901 gaataaactt cgaatatttt tttcaggtgg tttttcagca ccatttatag atactacaaa
    36961 gctaccatct gattttgagt cagtaatact ataaccactg tctattatag ctttcgaaaa
    37021 tctttgagat aaatcttttt ttacttcttc tctacctttc gcatcattaa tcttttgtga
    37081 gtaattatta aaaagctcga tagattcatc ttttttatct tcaacttgtt ctttcaacga
    37141 atctttacta atttgttctg agctacttaa gttcttatca tatacaggag acatacaccc
    37201 taccatcaat gctaagctaa gatttaaagc cacaatgatt gccactctat ttatattcct
    37261 ttttactttc atacacaatc ctcccatcac actaaccgct ctaaagtatt acttattgca
    37321 ctagaatatg tttatatttt ctttatatta aagatttatt attcaataca tatcaccttc
    37381 ctatccaatt cttataactt actattagta aaattataag ctccttaata cagaaataag
    37441 gatgttatct tttgttatta aaagataaca tccttatttt tttgtatcaa tacatatgtc
    37501 ccaactggta acttaatcgc cccaactgtc agtatcttgg tccaatggta tatatatatt
    37561 tagtataaat ttatcgatac tattttcaaa taataattct ccattatatt tttgtaaaga
    37621 agatttaata ctctgaatac ctattccatg tacaaactta tccattttgt ttgttaacaa
    37681 tatattttta ttaaaagtaa gctgatttag tttactattt tcacatttaa gtacaaaaaa
    37741 tgaccttgtc atagttcctt taattctaat atatttatta acattttcat ccactatttt
    37801 atcacatgct tctattgcat tatccaaaat attagaaaat atgcttgaca catctatagg
    37861 ttttacaaaa tttacttttg aaaaatttat agagcaagta aatttaattc ctttttttaa
    37921 acatatttca ctcttttcgt tgattataat gtctaatatc atatttcctg tattatagat
    37981 atatttaaat gtttttattt catcttttaa attgttaaca tattcattaa tttctttgct
    38041 attgtttcta aggttgtcta tacaataaat gtgattattt atatcgtgat atagtttctt
    38101 aactcgcata tgagattctt gaatacttaa ataatgcgca tattgcatat ctaacttatt
    38161 ttttattaat tcagcttgag attttaatct attctctttt attatttctt taaacaatag
    38221 tataagaaat atactactag ctcctaccaa ttttggatta acaaaattat caaaatagta
    38281 tccttctgaa tgtaatgacc caaaccgttc taattttttt aatacataat ttacaagcat
    38341 attcattgta ttaactaata aggcaaataa taaatataca taatttctct tatcttggct
    38401 aattaatttt atagacttcc taaaaaagct aaatataaga tatataagaa taattattaa
    38461 attactatat atctgaattt ttatagattc taagtgaggt actaataagg attctttaga
    38521 tgaaataaca ttacctgtaa ttgcaaaaaa tagtattcta tatataacag catccaaaca
    38581 tttataatat acactaaata gataaaagaa aatggaatac ttaatataac ccatcttata
    38641 aaaataatta tagtatcctc cccaagcaat aaatgctaat atataaaata aatagtttat
    38701 attactaaag taatttcctc cattaaataa tctacttatc tgaattataa tataaataag
    38761 aaacaagaca atatagaata ctattttttc atttttttta ctatcctttt tataaattaa
    38821 aattccgttc attatagaaa actgtataaa tatagataaa actactaata gctcagtcat
    38881 aaagtttagc atattgtatc accaagaaat tttaagaatt tctccttaac ttctttatat
    38941 ctatatctac taataggtac ctcttctcca ctttccagta ttgcagtatt aggctttata
    39001 ttttcaacat atcttaaatt tactataaaa cttttatggc atcttataaa tttatatgga
    39061 tttaattctt tttctatttt gcctaaacta tattttatat caaaattctt gtttattgtg
    39121 tgtattagca tatctttttt ttgaacttct atatatttta tttcatttaa ataaatctta
    39181 tatgtattag acttattttt tattgtaata tgactttcct tatttatttc aatatctttg
    39241 atacaagtta gcacatgttt ttttagttct tccaactcta ttggctttag taaataccta
    39301 taagctctta cctcataacc ttcttgcaca taatctatta atgaagttac aaatattatt
    39361 tccatttcat tgtctacttc tctaatcttt cttgcagtat ccattccatt taatttttcc
    39421 atttttatat caagaagtaa tatatctata tcttttaaat tactttccag taactcttct
    39481 ccagatatat acttttgtat ctcatattct atacttatat cttttaatat attctcaagg
    39541 taatcttcta ttaaaagttg tgtttctttt tcatcttcac aaactgctat cttaatcata
    39601 aaatgcctcc ttacataatt tctagttaat acaattaaaa tatttataat atatttaaag
    39661 ataattttgt atatctgata ctttaggata acattttcaa attattttta tcaatactca
    39721 atcccatata actattattt gatatttcaa tttaaagaca gaaattacta taattaaaaa
    39781 cagtacttac tgataaaaga agatgtctta aactattaaa aaaataattt taggacacct
    39841 tcttttattt atttaaactt tttaagcctt aaataataca ttaatctcct atataattgt
    39901 actgcatata aaatcaacaa tataatctgt agtacaatag cagttacaga tgctccataa
    39961 ccactatata aatttcttat ttcaatattc aataaatata taaatattac tactaacata
    40021 aagaatataa acatattcat aaataaaatt gactttactt tgtttttttt atcagaatca
    40081 ctatataatt taggaggtat gtctttgtat tctttcgacc atatatacca tgcagatgta
    40141 cccataaaac taccaatact cctaaataca agattccagc catttgattt atgttcatca
    40201 aaataattat tctctacatc atagtagtaa tcaacagtgt attttatttt tcttggactt
    40261 cccttatgaa aataaaatat tcctccaaat ttatttatct tatataaatt atatccttta
    40321 gcttcaatat cttctaacca tttctctatc ttatcaggtg aactatacca gtcaaatttg
    40381 aattttattt tcttttccat agcactcatt ctccaaaatg atatttttaa atagcttaaa
    40441 aatctattta aataattata tcattttgat actaatttta ggttacaatc atatatttaa
    40501 aaagctattt aaatagtaat aaactaatag ccataacacc cattcctgca atcaatccat
    40561 aaatagataa atgatgttcc ccgtactctc tagctgatgg caataattca tctagtgata
    40621 tgaataccat aattcctcct acaattgcaa atacaattcc aagagttata tcatttaaaa
    40681 aatttctaag aagtacatat ccaataatcg ctcctaaagg ctcagacata ccagataaaa
    40741 aggaataata aaaagctttc tttttatctc ctgtggcata ataaactggt acagatacag
    40801 atataccttc tggtatattg tgtattgcaa ttgcaattgc tatcggaata gctattgtaa
    40861 catcatcaag agctgatata aatgtagcta atccttcagg aaaattgtga attgctatag
    40921 ccattgctgt aaatatacct gttctcaaaa gagacttatt ttttttgata ttcttttctt
    40981 ctatattctc cattttcttt atttcatgtg gattttcttc ttgtggaatt agcttatcaa
    41041 taacagcaat gatagccata cctataaaaa aagctattac agttgaccaa cttcctaatt
    41101 tctctcccat agattttgtc aaagcatctt gtgctttagg aaaaatttct atcatagaaa
    41161 cataaatcat aacaccagca gaaaatccta agcttataga aagaaactta gtatttgttt
    41221 ttttagcaaa aaatgctata caacttccta taccagtaga taaaccagcc aataatgtta
    41281 tcaaaaatgc aaatattaca ttattcaaat aaatcacctt ctttttaatt gatattgatt
    41341 atcattttct ttatgctatc atatatattt tatatagtca atatttataa aaaatataca
    41401 caataaaaaa gcagagatat aaacttagtc tctgcttttt ctttatagta tattaaaatt
    41461 atttatttaa gaaatccact gcatattgca catataaagc agttccaatt tctagagcat
    41521 cttcatctat attaaagcat ccatgatgat gaggataaca agcacctttt gactcatttc
    41581 tagctcctac aaatgctagt gcaccaggtg ctatattcat aaactcagct aaatcttctg
    41641 ctcctgttac tttttcaaat agtgttatac agtcttctcc aaatagctta attgctgcat
    41701 ctgtagctat ctttgaacat tctttgtcat ttataactgc tggtgttaag tacccatact
    41761 ctaattcagc atctgctcta tatgcttctg ctgtagattt tgctatacgt tctaaaattc
    41821 caggaatttg ctttctaagc tctggattaa atagccttat tgtaccttct aagacagctt
    41881 cactcgcaat tacgttaaat ctagttccag aatttaaaac tccaacactc acaactaatg
    41941 gttccagagg acttacttct ctactaacca tagattgtag attcataact attgctgaac
    42001 ttgcaagcac tgcatcaaca ccttgatgag gaagtgaacc atgaccacct ctacctttta
    42061 cagtaatttt aaagaaatca gcagaagcca ttcttggtcc ttcttctacc gatatggtcc
    42121 cagattctac atcagtccaa agatgtattc caaacacact atctacacct tccatagcac
    42181 catcttgaat catagctctt gcacctttac caacttcttc tcctggctga aaaaataatt
    42241 ttacagttcc attgatacta tccttaatat catttaacac ttttgctgcc cctagaagca
    42301 ttgatgtgtg accatcatga ccacaagcat gcataagtcc ttcattttta gatttgtatt
    42361 ctacatcagt gcattcaaca acttgtagag catccatatc acccctaagt gctacagtct
    42421 ttcctggatt agctcctttt atagttgcaa ttacacctgt acctccagct gatacatatg
    42481 gtatccctat tttatcaagt tcttctttaa ctctctttga tgttctcact tcttccatac
    42541 ttttctcagg attttcatga aattcccttc taagcttaat tacatagtct ttataactct
    42601 ttgttatttc tttaatatcc attccatacc tccaagtagt acacattctt cttatttaaa
    42661 atacttttta tcacttaaat actatattag atttacaaag atacctgcta ttactacaga
    42721 agctattgta actgtagtaa atcctccaac aagcatcttt ggaagcatct tatccattaa
    42781 aaattctttt tcttctggtg tttcagccaa agcctttact gcctcttcag ttaatatata
    42841 gtttggtgga aaaccataaa gtgatgtaag cgaaactgaa aaagccattt ctttactttc
    42901 acctaaaagc ttacctacta taatagagaa tattcccata cctattacac caaatattat
    42961 gattcctaca agaggaactg ccatttcaac aagcattgct ggtgtagctt ttgctaaccc
    43021 tgcaaatata aaagccatca atgatgtcat taaccatcca aatgaaccag atttattaag
    43081 tggttttcta tcaactattc caagttctga tgctattaca ccaaatatta aacatataac
    43141 atatcttgat acaacttcat ttgtaagatt tgtaaattgt actgctaacc atgctacaaa
    43201 tcctaatcta agtattatca tatatgtcgt atcatatttt tctggtaagt ctggtattat
    43261 cttaaatctg cttttttgtt caggtaaatc cttaactttt tcttcatctg tatttacagt
    43321 tacttttcca cttctaaagt ctgataaaag tctttttcct tcttttttaa gcattaatgc
    43381 tgtaatagga taacccgcaa atccttgtgc aacatacatc aaaattgcaa gtactgataa
    43441 acttgcaaga ccttttgctg tagcagcatc tgccatcatt atagatgcaa ctagccctcc
    43501 agtaagagga ggtgtagcta ttactgctgt atcccatcca aatactactg tccctaatgc
    43561 caaagcacct acacatattc ctactattcc tgccaaggct attactattg ttttccattg
    43621 acctgctaat tccttaatac tcatcatagt tcccatatga gttattaaca aaagcatacc
    43681 taattgagct aatggtgttc ctaaagcggc taagtccaca atattttctg ggaaaaaagt
    43741 ccaaaatcct actataaata taattgctga tacaaaaacc gacggaacaa aagctttagt
    43801 ttttgctgaa actatatctc ctatcgtcag tgatactaaa actagacaaa aagctgttat
    43861 tgcctccata caaaatcccc cctcataata aatatatttt tataaaatgt atgtatagtt
    43921 atccattatt ttactacttt tgaaaaacat ttcaagtcaa aattcgattt ttttcataaa
    43981 attcgataaa ttccataaat taaagttagt tttctaaatt attctttttg taaaaacgtt
    44041 atcccgacca ttggctattt aactctattc ttacaaagag aattaaatgt aatttgcaaa
    44101 aaataaaaca taggatacat tttattcata aaacatctta taaatataaa atgaattgtc
    44161 tttatgaatc ttaatatatc ctatgtttgt aagtttaatg tgtatgttag ctatggtgta
    44221 aaatttgtat actagttatt gtgtaaccta ctatatactc catttttgtt tactagctct
    44281 tcatgagtac ctctttcttc aattccttta tccgtcaata ctatgatttc atcagcattt
    44341 tttatagtag tcaatctatg tgcaacaact aaatttgttc tatctttact taattcctct
    44401 aaagactctt gaatttctct ttctgttaca ttgtctaaag cagatgtagc ctcatccaat
    44461 attattattg gaggattctt taaaaagatt cttgaaattg aaattctttg tttttgccca
    44521 cctgataatt ttacgcctct ttctcctata taagtatcat atccatctgg caaagtctct
    44581 acaaagtcat gtattctagc ttttttagca gcagctatca tttcttcatc tgtggcatta
    44641 gggttcccac aaagaatatt atctcttata gttcctgtaa ataaaaatac atcttgttga
    44701 actataccta tattttttct caatgatttt aaactaacat cttttatact ctttccatca
    44761 atttttatat cacccttatc aaattcataa aatcttggca ataaattaca taatgtagtc
    44821 tttcctcctc cagaaggtcc aactaatgct atagtcttac cagcttttat agataaactt
    44881 aaattatcca aaacttgagt cttatcttca tatgtgaaag atatattttc tatctcaatg
    44941 tcacctttta cattttcaag ttcaatagga tttttagctt ctttttgatg gtcttgttcc
    45001 attatctcta tgaatctttt aaaacctgtc ataccatttt gatattgctc tgtaaagttt
    45061 attaattttt tcataggttg tataaacatt tttacatata gtatataagc agcaaaatca
    45121 cccaaagtaa tctttcctat ataagtaaaa taaccaccag ctattacaac tacccactct
    45181 aagatatcca tgtataaatt cataccagag taatattctg ccattacctt ataagaatct
    45241 tgtcttgctc ttctaaataa tttattgctt cttacaaact tatttaactc ttctttctca
    45301 gtacaaaaag acttagttac cttcatacct gctatactat tttctaaagt agcatttaca
    45361 tcacctatag taactctact cttcataaaa gcaatattca tcttatcttt tttagctatt
    45421 gcaaaccaaa ttataaacgg taatatagca aatattataa gcgtaagagg aatatttata
    45481 tctataagta taaagaatga accaaccaac attactatag aaatgaataa atcttcaggc
    45541 ccatgatgtg caagttctgt tatatccatt aaatcattta ctattctaga catagttact
    45601 ccagatttgt tattatcaaa atacgtattt ggaagtcttt gtaagtgtgt aaatacctca
    45661 tttctcatat cagcttgcat atctacacca ataacatgtc cccaatactg cataaaatag
    45721 ttaagccctg cttttgctat aaatataagt ataagtgcta ctgcaaaaac tactaacatt
    45781 cttaagtttt tatttggaac aacgtcatcc attatatttc tagttatcat aggataaact
    45841 aaatcacata aggaaaatag aaaagctgct ataaggtcta gtgtaaaaat tttcttatat
    45901 ggcttataat atttgacaaa ttgttttatc ataagtatta aatccctcct ttattagtac
    45961 ttctattagt acttctaaaa aatacccata aattagtata acataacaat aactttaaat
    46021 cttttataaa cttacaaagt aataacaatc tttttcataa aattgaagat ttatatcata
    46081 tagttgtata cttgaaacaa cataaaaaga gctatatctc tatatttaaa attttataaa
    46141 gatacaactc tatatttaat actaaatttt ttttataaat acaaaactac ttcaatgcat
    46201 ttaatatctt tttatctaat gctgttgtat ttatacctgt tatatatcct gttgccttta
    46261 ttgtaggtat atcataagtt gttggtagta tagtagtaga tacgattaaa tcagcacctt
    46321 cttgttttgt tgaacattca gcaattttac attgctcaac ttctgcttgt atattatttt
    46381 ctttaaccaa tctttctact ctgtcacaaa ctattgttga tgttgcgatt cctgctccac
    46441 aggctactaa tatttttttc atatattgtc ctcctacaat ttctttaata ttttataact
    46501 caaaataaaa ttgataaatt tcttatatct ttatttatat aaataattat atcattttat
    46561 ataagaagaa catcttaaaa ttattctttt aatatgtttt ttatttcaac attgcttata
    46621 cttaaaattt cattttttat acaagactct atgtactttg atatattttt ataatcattt
    46681 aaattaaact gttcaatatt caatttatat tctatatcac taacaatacc tatcaaatca
    46741 gacttattag ataaaatatc ctcatactta ccttttctag ttacttccag tcttttaaat
    46801 ctataatttt tatatccttc tatgataatc aagtccatat cttcatataa ttgaatcatt
    46861 ttatataatg gattcttctc ttttagttct tcaatatatg ctactcgttt atctgatatt
    46921 atacagactt ttctcgcccc agattttcta tgtttataag tatcttttcc ttcctcatcc
    46981 atatcaaaat catgacaggt atgtttaata actccaacac gatacccttt tttatttaat
    47041 tcttttatta tgccttcaat caacgtagtt ttacctactc ctgaatatga gacaactgac
    47101 aatatattat acataaaatt gtgtcctttc ataaaactat ctaataacaa catccactaa
    47161 ttgattttct tttattgtct cattcttttt aatcttaatc atacaattac ttcttagagt
    47221 tgcagatagt ctagcttttg catctttctt atcatttagc tctacataca atttaccttc
    47281 acttgtatat agttttccac ttacaaatct atctttattt gaaaatttat taaattctcc
    47341 tttaaatata gcttttatat gatgtgttcc cttcttatat gttaaatact caataacagg
    47401 aactatcaca ttatcaaata ccactattgc tgcatgtgga ttaccagaaa gaccaaataa
    47461 tagcttttct cccaatgtac ctacataaca agctccacca ggtttaatgt ttatcttcca
    47521 aaataattct gttgccccta tttttttgaa tacatctgga attaaatctt tatctccaac
    47581 agaaacacca cctgtggtaa ttaaaaaatc tacactctgt aacatattct ttaccttttt
    47641 ttctaactcc aatatatcat cagaacaatt gcatattgta tctgcttgta ttccaagttt
    47701 taataatctt gaatataagg tatatctatt actatcgtat attttaccat tctctaaagt
    47761 ctcatttata tcttgtagtt ctgtacctgt attcaaaatt ccaactttta aatcatcata
    47821 aacagttact ttggatattc caagacttgc taatactcca ataccatcat atcctatatt
    47881 ctgattttta ttaataatca attgcccctt ttttatatcc tcacccttaa atatgtaatt
    47941 atcgtattcc tttaatttta tatttaaagt tatcatatca cctataactg ttacatcttc
    48001 tttttttatt acacaatttg caccctgtgg tatctttcca ccagtcatta tcttcactgc
    48061 ttcattttta tttaataccc tattaaatac atctcctgca tacaaagtcc ctataacctt
    48121 taaatttcta ttttcagaat ttgcattata tgcataacca tcatagggtg acctatcaaa
    48181 tggaggacta tcaattgttg aatatatatc ttctgcaact accttattta ctgcatctat
    48241 aagatttact atcttgaccc tgtttattgg taaaacattt ttttcaataa tttttatggc
    48301 atcttctaat tctatcattt taaatactcc ccaatatatt ttttatttaa gatatttcta
    48361 tgtaatatat tatagattat aaaagttgga aatactttta tcttatattt aattataact
    48421 aatagttatc tataagtgca gttcaaattt tataaatcta ataaaaaaac tgttcccagc
    48481 tagaaatttc cttctatctt gaaacagcat catatgcttt agaatatttt attctaataa
    48541 agttcctttt attattttta caattttatc actttcaata gtttttatag attttatttt
    48601 gttatccaca atacttattg taagtacctc tttttttaat attttgttat cttctttcaa
    48661 tctataactc ttaactactt tactgccttc atgattatat gctgttaaat cataattcgc
    48721 atctcctaca atttcattta ttctggaatc ttcaacacca acatactctc ccaggtcaat
    48781 atgtctaaaa ttttctagtg atacagccga tttttcatgg tcactttttt caatcactat
    48841 actagattca gcatcaatga ctgatatacc ttgtgatgag aaagaattgg tttcaacatt
    48901 gacaacttta ttttcgttaa tatcaacata taaagcactt ccatctagtt catcatttcc
    48961 agtatatttt ggataaacta atgtcatata acttgatgta tttatatctt gcattgtatt
    49021 tgattctttt aaaactttag gttttatata ataaaatgta ttataaggta tacccatatt
    49081 ctttataatg tacttataat ctttattcat aatttcatta acatcatttt gaacttttgt
    49141 catagttttt tgctgtgttg ctttacttgc tacttgattt ttctctttag tattttcttt
    49201 atgacatcca acaagtataa tacctaaaat agttacacaa actaatgacc tttttaactt
    49261 cataaatcaa tcctcctttc ttaaatttta tagtatttat ctatatctca atttacagtt
    49321 atctcttttt tcaataatta tatttattat attataaaat aattatataa tatttatttg
    49381 aatttaataa gattaagaag ctaattgtaa tttttctgta ataaaaaaca ctttaaagtt
    49441 gaattttgaa aattcaattt acgatatttt tattgtaagt accttacaac attataccaa
    49501 attattccta aacaatcaaa aactccatga atatttaaag gttttctatt ttaaaaatca
    49561 cctttgacta ttaatttatc aaatatgtta taatagtttt agataattta aggaggatta
    49621 aataatggat tttaaacaac ttgaagtatt tgttgctgtt gcaaaacatc agagtttttc
    49681 aaaggctgca agagaacttt ttttaactca acctactgta agcgcacaca tacagaattt
    49741 agagagagag ctagaaactg ttcttataaa tagaagcaac aaagttatta cactcacaaa
    49801 atctggtgaa attctttatg aacatgctat atatatattg aataattgca aacgtgcaat
    49861 ttatgatata aaagaatatt caggaaaaat agaaggtatt atagatatag catgtagttc
    49921 catacctgaa acttatattt tacctgattt tatgaagagt ttttctatga gttatccaga
    49981 tgtaaaattt tccataagcc attatgattc tcaatatgct atatcagaaa ttttaaatga
    50041 acgaataagc tttggattag ttggttctaa aatcaataat cctcaaatag agtacctaga
    50101 cttattagat gatgaacttg ttcttatcac tccttcagat tttaaaattg ataataaaaa
    50161 taattgtatc gatataggag aattggctta tctaaatttt attatgagaa aagaagggtc
    50221 aggtacaaga aacttaatac taaacacact tagtaaaaat aactttccag taagtaaatt
    50281 aaatgtaatc gctcatgtag aatcaaatga ggctattaag gaaatggtta gattaggtct
    50341 tggagtgtcc tttatatcgt acatttcagc tatcgattat ctcaatgcag gtaaaataaa
    50401 atgttataaa ataaaggatg ttgattttac tagaaagttt ttcttcattt actctaaaaa
    50461 gaaaactttt tccccattag aagataaatt tctaaacaga ttatgtgagt atttcgaaat
    50521 tataatataa taactttaat aaagtagtgc ttaattttga gtttataact aattaataag
    50581 ctaattataa ctatcacata aatgcttata aattattatg aaccatcttt cttttttatt
    50641 taatttagtc aaaaaatccc tcgttcaaat gaacttggga tttttttatt tatactactc
    50701 caatagcagc caatactata caaataatca taactgaaat taatatcgta gttgtcttta
    50761 ccttttttcc taataaccaa tacataatac cgaatatcat agctggaagc atacatggca
    50821 ttatttggtc taagtaagtt tgaataggct ctgcccattc tccactacct acattaacac
    50881 taagttttaa cattacatta gatgcagtca tacctccaat taccataagc cctaacattg
    50941 aagctgcttc cataactttt gaaactatac ttgaaccact cacatcttta aagaattgag
    51001 ttccatactt aaatccttta tctagacata tatagcgtat aataaaatga ggaacattta
    51061 ttattaaaag gaataaaatc ggtcccatta tgttaccctg agaagctaaa gctataccaa
    51121 ccccagctgc tataagcttt aatgttcccc agaaaaatga atctcctatc ccagaaactg
    51181 gacccattaa acttgattta acagcattta tactattagc atcaaattct ttattctttg
    51241 cattttcttc ttccatagct ccagatatac ctaccaaaag agtacttata tgaggagtaa
    51301 ctgacataaa ttctaaatgt ctttttaaag ccttagattt cttttcttta tcatctttat
    51361 ataatctacg gattattggt gccatcatgt atgcatatgc tatattttgc atacgttcgt
    51421 agttccaaga tgaatctaat gtcaatgacc ttctaaaaca tttccaaaag tctttcctcg
    51481 ttattttact atctggtgaa attgtctcta acttcttaga actcgttgtc atctactatt
    51541 acctccttct ctcctacaaa gttaacatat attaaagcta ttgctaatcc aaacaatgca
    51601 actcctgtta ctgttatgtt taggtaagca accaatataa atcctaataa taaaaatgct
    51661 actgttttct tattaagcat cattctagct aacatggcaa aacctattgc tggaatcacg
    51721 cctgctgcaa catccattcc atgagttaca tatgatggta taaatgaaag tattttttct
    51781 attactggaa cacctaatga aaatcctata cctactatta ttgctaatat taatttattt
    51841 cctataccaa ataataattg cacacgttct acgccttcaa tattcccctc ttctgcataa
    51901 cggtcagcaa tatgagctcc ccatgtaagt actactgtca ttaagaagtt atctataagc
    51961 aatactagag ttgctatagg aactgcaagt acaactgctg ctgatacatc ttgacccgtt
    52021 aatattacaa acgctgtggc aataatagaa ccagacacaa aatcaggagg attacttgcc
    52081 ccaacaggaa atgcacccat aaatactaat tctaatgttg aacctattac tatacctgta
    52141 tgaagattac ctagtgctaa accaacaagt ggtgcaacaa ctataggtct attatgcatt
    52201 gtagaaccta caaaactatg catatatgca aagaatgcaa ttagagttat caaaataact
    52261 tgtgtaaatt ccattttcat ccccctaaaa ttatattaat ttcattacat ttgtagcatc
    52321 atcatcagga actaattgta caaatacatt tacgccttct gaatctaact ctttaatcat
    52381 ctttatatca tcttttgata catgcactgc tttagatata ttttcacgat tatcatctga
    52441 ttttgttcca ccaaggttta tagatttaat agcttttacc tctttagcaa gtctataaac
    52501 atcttctaca gattcacaaa gtatcaataa gttgtattta tcagtaacac ctgagttaag
    52561 tgcttttata gaatctgcta tgcttttcat tactaactta acattagatg gtttagccat
    52621 tttaagtcct gccattctca attcatcttt taataagtca tcacttgcta tcaatataca
    52681 gtctgtgctt aaaaactttg tccatgtaaa tgctacttgt ccatgtataa gtctgtggtc
    52741 tactcttact aatttaatca ttataattcc tccctaattt ttttgatttc atccatgaat
    52801 gtttttacgt gttctacact aacatcccca aaatctttgt aattatcttt aaagtaactt
    52861 cctattattg ccatatctcc taatttaagc tgtttttttg cattttctaa tgtaactcca
    52921 gcagctataa ccaatgggaa gtctcctaaa tttttacgaa acagttctat tttttccatt
    52981 gatgtttctt gacctgtagc attttctgta actgctatag catcacatct tgtcattcct
    53041 atttttaaat cttcttcaac atcattttct gataatactg gctgatattt aaaacgtacg
    53101 cctccaatta aatacgctgg gcattttgaa cgttgtaatt taaaaaactc ttctaaagta
    53161 gcttcatctc taggtttaac atgacctact acagaatcaa cctgtatata actagcatta
    53221 tattttgttg ccagttcaaa tcccatagta tcaacattta aacagtttac tccatatggt
    53281 attgataaat tagcttttga tacatattca agtattcttt ctaaatcata ataattacca
    53341 tagtaatttt ctaacattat tccatctaca ccattttctt caaagattgt aatctctttt
    53401 ttagctcttt caaagatatc ttctggagta tctcctttta agtgtatcat tgctataatt
    53461 ggttttttac ttttaaatac tgataatatt cccttttttt tcataaacta aaatacctct
    53521 tcttcttctt taatttgtaa tgtcacgctt tgtattcctc ttctagattc ttccactaat
    53581 tggtctatat cttcaacggt taattcatca gaaaaccttg taagaagttc taataccata
    53641 ttcaaattca ttccagaaaa taacctaaca ttatgtagat tagttaatct taacattgct
    53701 gtacatacac ttgctccata taagtcagat aaaattatat attcacattc tttatcttct
    53761 gatattgttt tttctaattc taaagcaaaa tctgaagcac tttgtccagg gtacaaacca
    53821 tacgaactga cacaatctgc taaatctcct actatcattt ttactgcatt taaaagccct
    53881 tttgaaaagt ctccatgaga tgctaatatt atctttttca tactcatcct cctactaaat
    53941 tatagattat atttctgctg atactgcata tttgtactta tcacttctta taaattgctc
    54001 attgtaatat aagacatcat tattttctgt atacaccgat tgtcttactt tcaatagtgg
    54061 catattactt gcacatttaa gatggtcaga atattctttg tttgaatgtt ttacttcaaa
    54121 tatattttct actcgtttag gtaatgtgct tatattatta gatataacct ccattaaaga
    54181 tttatcttct aattcttctt ttaataaaaa catgtaatct attctaaaat agtcaacttc
    54241 aaaaataaca ggtacttcat ctattaatct aagtctttta atacatatta ccttctcttc
    54301 tagttcaata cttaaagctt cagctacttg cttatctgct ttttttatta aaacagagat
    54361 tattttggta cttggttttg cattaatacg atgacaagaa ttagtaaaac tatttcctgc
    54421 acatgttgct tctattctct ctggtattgt tacataagtc cctttaccac gttttttaca
    54481 taatatatct tcttttacaa gttcattaag tgcatttctc acagttatac gacttatatt
    54541 aaattcttta cataagtctc cttcagatgg aagtttatct cctggtttat atatgccaga
    54601 tgctatttgt ttttttatgt tgtcatatag ttgttgatat aaaggaacaa aggaatttac
    54661 ttctaaatcc atttcttttt tttgtctctt ttcatccatt ttgctcacct ccctatgtaa
    54721 catttttata acattatata acaagttctt tattacatat tataacaaga tgaaatcgtt
    54781 gtcaattttt tttaaaataa tttttaattt gacagtattt tcttttattt tctctagtgt
    54841 gacaaattgc tatttttaca taaaaataag actgttgtac aacagattaa attgtttata
    54901 caacagcctt tattcactta ttacatttat atttttactt ataaatttat attaaaaaat
    54961 gccttatatc ccttatacat ttcatatatg tctactttag agacaatctc atgtggtgca
    55021 tgcatattta aaactccaac accacagtct ataacctctg caccttgatt tgccaatata
    55081 tgggctatag ttccgcctcc tccttggtct acttttccaa gttcagcagt ttgccatact
    55141 acatttcctt tattaaaaat tctctttact tctgacataa attcagcatt agcatcatta
    55201 catccactct ttcctcttga acctgtatac ttagttaaaa ctactccatt tcccatatat
    55261 gccgaatttc tcttttcata tgcttctcca aaatttgggt catatccagc agaaacatca
    55321 gctgataata ccttactatt tgctaaagct cttcttactt taatatcaca ataatctcct
    55381 tctaaagcaa tcagttcagc cacagtattt tcaaaaaact ttgagtgcat acctgtattc
    55441 cctttagaac caatttcttc tttatctaca cataatgcaa ctgaagtaaa ttctggttgt
    55501 tcagtttcta gtatagcttt tacagcagca tatgaacata ccctatcatc atgcccatat
    55561 cccaacacca tagacctatc aagccctaaa tctctagcct taccagctgg aactacttca
    55621 atttcagcac ttaataaatc ttcttcaact atattgtatt tctcatttaa aatctttaat
    55681 atatttgctg ttataggttc tttttcttca tcttctaaag gcatattccc aattaatata
    55741 tttaaagctt ctccactaat tccttctgat agtttctttt gcatttggtc tccagccaaa
    55801 tgaatcaata aatcagttac acaaaatact gggtcactat cttcttcccc aatagatatg
    55861 tccacttttg ttccatcatt taatataact ataccatgta aagcaagtgg aatagccgtc
    55921 cattggtatt tttttattcc accataataa tgagtcttaa aaaaacctag attactctct
    55981 tgatacagtg gatttggttt taaatctaat cttggcgaat ctatatggct tcctataatc
    56041 ttcatacctt tttctatata atttttacct attacaaaaa gtgctactgc cttctcttta
    56101 tttataacat aaattttatc accacaactt atgcttcctt tttccatagc ttcatctatt
    56161 gaaatatagt tattttcttt agccaatttt ataatttctt ttactgaaag tctttcagtc
    56221 tttgacttac ttaaaaactc catataattg ctagaatatt gcataatttg ttcaatagta
    56281 ttttcttttc tttcaaaatc ccaagcattc ttaaattcgt actttaagtt catattttcc
    56341 tcctactaag ttctaatttt tattgttttt catgtattgc tcatattctt ctggtgaaac
    56401 tactttgaca ctatctagtc ttgatctaaa gtgggctcta aagttttcta aatattcttt
    56461 ccttaaaatt tctctttctt tgatttcatc agcagaaagg attccctctt tatttttctt
    56521 tgctaattca ttaatcctat ctaatttctt cttgtcaaac attttttcct cctaaaaaac
    56581 ctctatttat ttaataaaat aatttattgc tttttatatc taacttgttt ttatattcac
    56641 aaaaaaactc atctatatta tcacttatta tagatattcc cttttctagt tcttccatgc
    56701 ttggacgagc tatatttatc ctaaagaatc tatcatccac tagattgtca aaaaaataag
    56761 tccctggaag cattgacact cctttgttta acataaaatt tgtaaatgct tgagatgtat
    56821 atcctcttgg taattctaaa aaaaagttta ttcctccaca agattttcta acttttaatt
    56881 taccactcaa tttgtcatta atcaagtttt ttgccaattt atacttatca gtatatatct
    56941 tctctatatg actcaaataa ttcttccaat caaaattatt catatagtaa tacattgatt
    57001 tttgaattaa acctggtgtt gatatatctg atgaatattt agcccataaa acttttttta
    57061 acaactcatt tggcatttct acaattccta tcctaagacc tggcataagt atttttgaaa
    57121 agctttttat atatactact ctatttttat catcataact tctaattgtt ctattgtcct
    57181 ttgattcaaa aacaaaatca cttataaaat catcttctat tatataaaaa tcatgttctt
    57241 ctgccaattc tatcaatttc ttttttttat atgttgagta agatattcca gttggatttt
    57301 gaaaattagg cataatgtat ataagtcttg gttttatttt ttccaacttt aattttaaaa
    57361 tacctatatc aattccatca tctaacatag gtatagatat tatttttgca cctctacttt
    57421 taaacacttc aattgctcca ttgtaagaag gttcttccat aaaaacaaca tctgaataac
    57481 ttataagtcc tttacaaact atatctatac cttgttgagc acctgatatt atttgaatat
    57541 tttccttagt agtttttata tttccatcac ttagatactc taccattttt tctcttaatt
    57601 cttcagagcc aagaccttca tcatattcaa atattgaact accatcttta gataatgcca
    57661 tatttacagc atttttaaat gaatcgatag gaaacatatc attagatgga ttcccattgt
    57721 caaaatgaat taaatcttct ctttgcacaa taatcttatt tttcctgtta tctataccgc
    57781 ttttataaga atttaaactt gatacaaaag taccactccc tataatttta tatacatatc
    57841 cttcattttc taaaagctcg tataccttaa ctatagtagt gttatttact tgtaaaaaat
    57901 ttgatagttt tcttattgga ggcaattttt catgttctac taactcttta tttattatca
    57961 attcttttat atgattaaat atctgtatat atttagttga ttttttctca tttaaatcta
    58021 ttctgtattt ttccaaattt atcaccgctc attgtattta tattttattg atgcttttat
    58081 gtatatttga tattatttag tatgtatata taataccaaa atttttataa aaaggggaaa
    58141 tatttatgtt ttacgactat cacatgcact caagtttctc tacagacgga aaatctacaa
    58201 tggaagaaat ggtaaaaaaa tctattgaac tcggtttgga ggaaatttgt ttcacagacc
    58261 atgttgacta tgatgtatat gctgatgatt ctttttcaat tgtctacgaa gattatttta
    58321 agagtttaga gactcttcaa agcaaatata aagataaaat atcaataaaa aaaggaattg
    58381 aatttggagt tcaaactcaa ttaatggata catacaaaaa ggaagcacat caatatccat
    58441 tggactttat aatatgttct atccatgcta tagatacaat ggacttatac cttggtaatt
    58501 attttaaaga taaaactcaa catgaagttt atgaaaatta ctatctttat ttatataata
    58561 ttgttaagaa ttataaagat tactctgtct taggtcattt agatttgatt aaaaggtatg
    58621 caccttatga cactatttta gatgatagac tattttctga tataattgaa gaaactctta
    58681 aacaagctat ttatgatggc aaaggtatcg agataaatac ctcttgttat agatataatc
    58741 ttccagacct aaccccatct aagtatatac ttcaaatgta caaagacttg ggaggggaaa
    58801 tcataaccac aggttcagat tcacatcata taagtcaagt tgcatgtgag tttgactaca
    58861 tatattcatt actaaaaaat atgggattta aatatgtatc taaatttaat aaattgaaac
    58921 cagaatttat aaaattataa atttttacac ctccttgtga atatatttaa aagataagga
    58981 ggtgattttt ttgaaagtac cttatttagt actatcaaca gttattttat ttatatccaa
    59041 ttttattgta agaatatttg gttttttata taaaatattc ttatcaagag cactaggtga
    59101 aacaggttta ggtatctatc atatgatttt taactttttg atgatttgct tagctgttac
    59161 aacaacaggt atacctactg cacttagttg tctagttgca aagagaaaag ctttaaacga
    59221 tagacacaat actaatgctt tatttatatc aactttatat atatcatttt tcgtagcact
    59281 aattatatct atagttgcat ctttcaatag ttctttttta tcacttaaat ttttaaaaga
    59341 tgctaaatta aatctattta tactagctgt ttgtcctgct atagttataa taactctttc
    59401 aaacgtacta agaggatatt actatggcat aaagaatgta aaaatccctg ctattggaca
    59461 aatcattgaa caaattggta aaatattatt tgttttttta cttgttatgt acataaataa
    59521 taaatctatg aattgttata tagctctatt aggtatatcg attggtgagt tgagtaatat
    59581 tatatttatg cttatttgct tatggcgaga ttcatcattt gacaataggt atattataag
    59641 cataaaagat ttttataatt cttctatgga aacattaaaa atgtctattc ccataacttg
    59701 taatagaatg tcaaacatac ttttgcaatc tataagttct atgatgatac catcaagatt
    59761 agcattatca ggaatgactt atcaacaatc tttaagtatg tatggtattg tcagtgggat
    59821 ggtaatgcct tttatatttt taccttttac tgttggttca gcgcttattg taaatttaat
    59881 accaactatt tcacaagaaa tggctctaag aaagcgtaag agtgtaatta aaaaaatcaa
    59941 atactccata cttcttactt tatttgtagg catactatca tcaatatttt tttatttctt
    60001 tggaaaagat ttatgtatat tagtatttaa aaatacactt gcaggagaat atctaaaagc
    60061 tatgttttta gttccactat ttatgtccct aaatcaaact ctatctggca ttttacattc
    60121 aataagaaaa gaattagctt caagtataaa tactattact ggcatgttaa ttcaactaat
    60181 agctttatat gtatttcttc caattccagg acttaatatt tatgcataca tatacacaat
    60241 gacgatagta tcaatcttta cttgtctatt acatactata gtgcttttta aatcactaaa
    60301 atctatacgc tagttataaa aacgtgaagg ctaccaatgt aaattataca ctttggtagc
    60361 cttccttaga tgatttttat ttataattta aactgtctat atatctacct atctatctgt
    60421 ggcaacttgg ctttccactt ccatgattag gtccacattg cgaaaaatca catactttag
    60481 agaaagtatc aacaagtctt ggttggattg gatttggtga tgctaaaact actatttgtt
    60541 cacttattaa tgtgctaact gtttcttgta agcaaagtac taaatctata tcatttggta
    60601 taattaattc atagaaaggt tcacaacatt cgttgcattc taattttaat atagattttc
    60661 ctatacaatc tacagttaag ttagtttgat attcttgtct taaagtaaca gctcttccgt
    60721 cagctggcaa gcatattggt gctgaaagtg tgttaaaagt aaattctaca taatcaactc
    60781 ttctcttaca tccacctcct gctctaactg ctgggaaagc taatgcaacg aactctgtac
    60841 atccacatct acctcttagc tctagtacta aattacgaac tgctacagtt aatcctcttt
    60901 gtttataagc tactgatttg ccttttcctt gtctacagca ctcagagttt ctttctccac
    60961 aaactgcaaa ttcaaaagtt gtttcacagt ttcttcctac tatatctcct aatgggtcta
    61021 aatcgaaatc ttctaaagtt gtcattcctg tgtctataac gattccatca ttacttaagc
    61081 atattttttc tatagttaca cttgcttgac ctgcacttgg aactgaacca aatacttcta
    61141 ctacttctgt ttcaaaagtt aatggctctc catttggtcc tgttgcatct gtaaatgttt
    61201 ggaattgcat agtatcaaat attctattag cctctattgc aaatggcact gcatttgata
    61261 cagagaagtt tcttggagat ataggattac agcattctgg tccacatttg ttttcaaaac
    61321 attcatctga atccatttca catggttcac aatcacattt acaattgtca tggcatccac
    61381 aattgtcatc acatggatta ggtttgcatg gattgcatgg gttaggttta catggattac
    61441 atgggttgca tggattgcaa tcatcatggc actcacactt attatcagta ttacatccat
    61501 tatgatggtg tgatttttta aaacctctca tgtcttctga atttatttca tctgtataca
    61561 cttcttcttc attcattgta gacattggct gattcatcat tcttctttta ttttttttat
    61621 aatcttgcat ttttcattgc cctccctgta agcgattctt attaagtctt tggctttttg
    61681 aagcctaatt aatactatgc ttttataatc ttaattgtta cttgcttttt gaatttatca
    61741 ataattaggc gtatttagta gaaaaatgca tattaataaa aaaagtacta ctatttgaaa
    61801 tagtagcact ttttttgtcc cgaaacaccg gtggctaagt aaattcacac cctaggcact
    61861 cccaggtggg tgacttgcca gtaaccttta acgtccaaac taactaggca tgttataatt
    61921 taccagacac ggtctcccgt atttcatttg taggttgaat ttaaaaaagc cttatcgtat
    61981 gtttcaggat ttcatattat tattatataa taaattttga attttttaaa gtggttttat
    62041 atagtaattg agataatctt attctatcat tactttttct tccttttttc tttatgtatt
    62101 tagttaatta tcgttttttc ttagctctag ttttagtatt tttttcatta taattgtatt
    62161 cggattcttg ctcattaatg ttgtatagtt cctcatcgta tactatatct tcatcatttt
    62221 ctatattttc atagcctgtg ttcttatctt ctactaattc atcttcatta tcctccattt
    62281 caccttcatc tgttaactca tcatcatcca attctttgtc ttcacaatct aattcttcgt
    62341 cttcataatt taactcatca ttattgtctt catcatcatt attactatca tcacctctta
    62401 aaagacttcc taacaatttg agattattca tactaccaag attgtttata tttgccaaag
    62461 agctcaaact aaatccacct tcaccaaagt catctggtaa aaataattct gcaaataaat
    62521 ctatcttctt tttcattcct atagcttttt caactgaatt ttttcttttt tctgggaata
    62581 catctataat ttcctttatc atcatattct ttttatcatc actatccatt tttctgaaaa
    62641 aatccgtttc atcttctgta tctaccatat ctatattatt taattttttt attcctctaa
    62701 ctaaatctaa aacactttct atttttatta gtatttttct ttcatctctt gacatgtatt
    62761 ttttagttct tgttattatt tccattccca tttctaaatc ttcttcactt aaatccatga
    62821 gccttaaacc tctttttata tcatttgcat caaaccttaa attgccatgt gtagactgtg
    62881 aaagtttatc atttgcattt tttttgtttt ttctaactct attatttcct tttgatttat
    62941 ttttatcatc aaatatagag atattaccat tactcaatgc aatcactcca atcaaaaata
    63001 gtaagttatt caagacaatt cccccttata ttatataatt gccctatatt aaagtatgtt
    63061 tttcatattt taatgttcca tttttaaagc tcagtgcata tatattatct ataagaattt
    63121 ttatttatat tatatatcac tcacaattca ccacttcaat attatttcta tatataaata
    63181 gtatgaatat aattcaatat atatttatag aaaggggttt ttttatgaaa aaattagata
    63241 cagagtcatt agaaaaaatg gctaaacaaa aaaatattga caaggataaa atagaaaaga
    63301 tggctgatag ttacaaagga aaatcagaaa atgaacttat ggaggaactt ataaaaatag
    63361 gtaagaatct agatggcaga gatgaagtag tatcaaaatt caaagcattt ttagatgaaa
    63421 atcaacgcaa aaaactggat aatataatgg aaaaaatctc agatgcagaa aaccaaagag
    63481 atactaaacc agctaaaact aagaaggcta aacctactaa aggtaagtca aattcacctg
    63541 caccaaaaaa tacaccaggt agtcaacaaa aaaaatctaa aagtttattt aagaaaacta
    63601 aaaagtctaa tcctcatgaa tagataaaaa taaactattt aagcaattag ataattattt
    63661 ttagttaatc tattattaaa tttgatatta ttgacataaa aaatcaggta gtgcctaagg
    63721 cactacctga ttgattttaa tagttttatt tttttctttc tgtaacactt tctctatcaa
    63781 caattctata aggtaactct attttcttat tttcaattaa ttccttattt attaatttta
    63841 taaccattct tattgctact gctcccatat cgtatagagg ttgatatact gtagtaagtt
    63901 taggtctata cattctagct atcttaacat cattaaatcc tgcaactgat atgtcttctg
    63961 gaaccttata tccagcatca catatagcat ttatagcacc tatggcagcc tcatcacctg
    64021 ttacaaaagc cgcatgaggt attattccat catctaatag ttctttcata ctattgtatc
    64081 cactctcata atcagttcca ccatacttaa tcaaactctt gtctaattct atattgttat
    64141 ttgaaagtgc tttctcataa ccagcaagtc tttccatttc taaaacagta tcatctttac
    64201 tagtcattat aaaagctatc ttttcatgac ctttctttat aagatgctct gtcatatcaa
    64261 aagtagcttc ttcgttgcta gtacttactg tatatatatc ataatttcta gcagttttac
    64321 ttatatatgt cgctggtatt ccacaatttt gtatatagtt gatatgttct tgttcaacta
    64381 tccatgaaat cataactata ccttccactt gttttgctct caatagattt atactcttaa
    64441 gttcttgttc cttatcagag tatgtattcg ctaaaagaat atcatagtca tacattttag
    64501 caacctcttc tatcccattt aacacctcat taacaaaaga atctgaaact tctggaacta
    64561 ttacccctat taattgactc ttctttgtta ctaagcttct agcaagtgga tttggtatat
    64621 atccagtctc ttttataacc tctaaaactt tttgtttgac ttcatcagtt acaggttttg
    64681 aatcatttat aactctagat acagtagata ttgacactcc tgcttgttta gcaacatctt
    64741 ttatcgttat attgcctttc atcttcatcc tccttatttt actaaattta tctaaaactg
    64801 aaccaaattt ttgaaggttc ctattttaac tacatttata taagttttta ttttaccata
    64861 aaagcaagta catttaaata cattatattt tatattatac cctatattca acaaatttaa
    64921 taatattcat tcttaattta aatcatatta aaaaataaaa taaaaaaatg ctggtataaa
    64981 ataccagcat ttttttattc taatgattta agtattagca gccgcaacct acttcaccat
    65041 caaagaatcc accacatgaa caagttatta gcaataatat taagaaaggt atccatgaca
    65101 ttatgtcaat gtctctccag caatcttcaa aagctaagaa taggaagaat agtactatta
    65161 tccaccatcc tcctccacca aagcatcctt cttctccaaa aaatctatct aacataacaa
    65221 agccctcctt tttttaataa ctgtgttctt attctaagca tccaccacag cagcaaagta
    65281 ttaataaaac tattaggaat ggtatccatg ccattatgtc aaattctgcc cagctatctc
    65341 caaaagctaa gaataggaag aatagtacta ttatccacca tcctcctcca ccaaaacatc
    65401 cttcttctcc aaagaatcta tctaacatta tacgttacct cctgttgata ttttgttgag
    65461 cttttcgctc ctaacatata ctatgcattt ggtttccatt ttgctaatgt atttttacta
    65521 tttttttaaa ttttttaatt tgaactaaaa aaggtaaaac cataacttgt ataagttact
    65581 ggttttacct atttaataat caatattttt tagcaacaac aaccatcatc gaagttaaat
    65641 aataacaata ctattaatat aacccatatc catatattgt catcgcataa taagtcttct
    65701 aaaatgtctg tgcataatag taagaatact ataactatta ttatcaacat tttattccca
    65761 aaaaatcctt cgcatcctcc aaagaaacgt tccatattgt cttctaattt tgtcataact
    65821 aatcctcctt gaatcattaa gagactaagt tttgtctctt taattaaata tatgtagtaa
    65881 tagacagata agttacttct aaaaagtttt tattgattag tttatttatt tctttttcac
    65941 caagtttatt gtatctatca acttcatcca gataatcttt tttctcctct gatatagtat
    66001 agattaaact ttctattgat tttattttgt actccttata tctttctatc aagaaggatg
    66061 tttttgaatt ttctattcct ttctttacat gcattaacct tgatatgtaa tcttcttttt
    66121 cattagatac ataactctcc ttttcagcta catcttcaag gattaaattg gtctccatta
    66181 cataatcctc tatacgttgc atatctaaag ttattgaatt ataataaaaa tttactgcta
    66241 taagtaaaaa cattcctact gaagttagta atagtcctac atatttcata gcatttcctc
    66301 ctaagttata tttgttatca ataaagttta ttcatcaaat aatactttat tactacattt
    66361 ataaaatttt attatttttt atagattcca tattgaaaaa ttcttgatat tatattaaac
    66421 taacattaaa attaattaaa actgctttag gaggcttaaa atgtttgaga aaataaaaga
    66481 aataatagca gaacaattag gggtagacaa tttagatgag ataactatgg aagcatcttt
    66541 aatggacgac ttagaagctg attcattaga tgctgttgaa attataatgg cccttgaaga
    66601 tgaatttgga atagaaatac cagacgagga agctgaaaac tttaaatgta ttggtgatat
    66661 atgtaaatat atcgaagaaa acaaatagta tcgttttgtc tacaaatata aatagtctgt
    66721 gtaaacttct acataataaa ttatattaga gttcgcacag actagattac atttatttac
    66781 aatatataaa atacacgctc ttatatttaa tttatatact aagtatataa attacttttc
    66841 ttcataaaac gtaacattct ttattattat atctatagtt ccatcttgat tttgttttac
    66901 agtatacttc attgggtctt caaaatcagt tagattccct tttatgtcaa aaccattatc
    66961 cgtttttata cttctttttt taagcttttt ctcaacccat tttttatcta tactaaaact
    67021 ttcgtcaaga cctttttctt ccatatgctc tttaaagcta tcctttaact catcatcttt
    67081 aattgaatta tcaacaaagt catttatatc aacttcatgt ttttccttca gtgtataatt
    67141 taatatactt cttacatctt cagcttgctt tatatcatta ctaagagcat tggttatcca
    67201 attttcagct gtatttttaa ataccttggt cttgtactta tcatcatcta tttttttagc
    67261 atttaaaaat tctgttacaa acttagattc tgacccttct ttttcagcat ccttatctaa
    67321 aagccttaga tgaaactcat catttatacc acttagacta atcaaagctc cttgcttttg
    67381 tcttaaagtc tctgggatac caatttcatt tgaaaccatt tgaatattaa atttatcatc
    67441 cacaaattca attgaatgag tgtacaactt tttataatct aatttcagta ttgctacact
    67501 cttttcatct ttaatagtgt ataagcaaat tgccaaatca caagattcta gtgcagcatt
    67561 aattttcata acatcaaaca agtaagatgc aatttcttta gaatttttca aaaaagtact
    67621 ttcatcatat attatttgtt cacaacagtt tttaattaaa ttgtcattat aatctttaaa
    67681 aacacctttt cttaaatcct catcttttgc aattcttttt atagctttct ggaaaaaacc
    67741 atctacttct tgattaactt tgccttcaaa gtcatttaat attggaacat cactattttt
    67801 gtccaaaaca tgtattataa atttatgtat tatcatatat atcaccccat tatcttaatt
    67861 tcgatattaa ctctttcata tcctctggta tatctgcttt aaatttcaat ttttcttttg
    67921 ttcttggttg ttcaaactct agcttatatg catgaagtgc ttgcctattt attagatctt
    67981 catctacata accatacaac tcatcaccta ttattccatg acctatatga gccatatgta
    68041 ctcttatttg atgtgttcta ccagtttcta agcttacttc taatacagtt gcattttcta
    68101 gtctttctat tactttatag tgagtaacag atgactgtcc tctttcatct attatcctct
    68161 ttattgaatc ttctgtaggt ctataaattg gctcatctat agttccctca ttttctttta
    68221 taattccatc aactactgtt atatactttt tttcaacttt attttcactc attgctgtag
    68281 acaaagtatg atgtgcataa gcattttttg ctactataac taaccctgaa gtattcatat
    68341 ctaacctatt tacaaatctt atttttacat tttctttttg attatctata tagtaactaa
    68401 taccatttgc tattgtttta tcatagtggc tttttgttgg gtgaactacc ataaacggag
    68461 gtttattcac cataattata tcaaaatcat cataaattat ctgtaaattt aaatcttgag
    68521 gttcaaaatt tgctttttcc tcgtctattt ttacttctat taaatcacca ctatacacct
    68581 ttaaactcgg ttttttatat actccattta ctaaaacact cttttccctc ttcatttttg
    68641 ataatgacct aactgagaaa tttagcttgt ccaataaaac ttcttttaaa gtcatttctt
    68701 cttcatttgt atacgatata agattatatc tttgattctc ttttttaaac aaacttttac
    68761 ctactttctt ttatatcttc ctactttttt atatcttcaa aatcatgata ttatatattt
    68821 tacactattt ttgtttaata gataataatt atttttaact ataaaaacct gtaagaataa
    68881 caaatagtta ttcttacagg ttaaattcat aattaaatat ttaatttgta aatattataa
    68941 tctaaaacta tctcttaact attaaagtag ttcccattcc tccacctata caaagtgtag
    69001 caagaccagt tttagcatct cttctcttca tttcatataa aagtgtagta agtattcttg
    69061 ctcctgagca tcctattgga tgtcctatag ctattgctcc accattaaca ttaactttgt
    69121 tcatatctat atttaagtct cttattacag ctacagattg ggcagcaaat gcctcattag
    69181 cttcaactaa atctatatct tcaatagtca tattagcagc ttctaaagct tttttagttg
    69241 ctggaactgg tccatatccc attattttag ggtcaacacc agctgttcca taagaaacta
    69301 tagttgcaag aggctctatt cctagttctt cagctttttc tttagccatt actactagca
    69361 tagcagcacc atcatttatt cctgatgcat taccagcagt aactgttcca tcttttttaa
    69421 atgcaggtct taacttagca agtttctcca ttgtagtgcc aggcttaata tattcatctt
    69481 tatctactac agtgtcacct tttcttcctt ttataacaac aggaactatt tcttcatcaa
    69541 attttccttc agcttgagct ttttcagctt tattttgact tgcaagagct aattcatctt
    69601 gttcttctct agttatattc cattgctctg ctatgttttc agcagtaata cccatgtgat
    69661 agttattaaa tatgtctgat aatccatctt ttatcattga atcaacaaaa gcagcatcac
    69721 ccattcttgc accatatctc gcacttggta ctaaataagg agacatactc atgttttcag
    69781 ctccaccaac taacattata tcagcatcac ctaatgctat aagttgagat gccattgaaa
    69841 cagatcttaa tccagaacca caaactatat ttatagtcat agctggtttt tctactggta
    69901 ttcctgctcc taatgctatt tgtcttgcta tattttgtcc aagacctgct gtaagtactc
    69961 cccctaaaag agattcatct atcatatctg gagttatgtt agctcttttt atagcttctt
    70021 tagctgctgt tacccctaac tctaccgctg aaactgattt aaatgctcct ccaaaacttc
    70081 ctactgctgt tctagctgca ctggcaatta ctacttctct catattcatt acccccatta
    70141 aattagattt ttcatatatc cgaaattgtt ttttaaacaa ttttatttaa acttttaatt
    70201 cccaaattct tatttattat aatcatagaa tcctatctta gtttttcttc ctaattgatt
    70261 agctctaacc attttagcta aaagtggatg aggtctatat ttagtatctc caaactcagt
    70321 atataaaacg ttcattatag ctaaaacaac atctaatcca attaaatcac ctaatgctag
    70381 tggtcccatt ggatggtttg ctcctaattt catagcttca tctatttctt ctttacttgc
    70441 aacaccatct gcatatatac caacagcttc atttatcata ggtataagta ttctatttac
    70501 tacaaatcca ggagattcag atacatctac tggtacttta ttgatactct tagataattc
    70561 aaatactgta tcaaaagtaa cttttgatgt taactgacca cttataactt caactaattt
    70621 catcatagga actggattaa agaaatgcat tcctataact ttatctggtc tcttagtaga
    70681 agaagctatt tctgttatag ataatgatga agtatttgtt gccaagatag tatcttcttt
    70741 acataattca tctagtaact tgaaaacatc tttctttata ttcatgtctt ctacagatgc
    70801 ttctattatt aaatccatat cttttaaatc ttcatagtta gtagttgaac taacatgact
    70861 taatatttct gcttttgtag cttcatccat ttttccctta gtaactaact tagttaaatt
    70921 tttatctaat aaagctaaac atttatctat agcaccttga gttctactct ttaaacatac
    70981 atcatgtcca caacttgcaa aagtttgtac aataccactt cccatagttc cactacctat
    71041 tacagctaat ttcatagtta taacctcctg ttattttagt atttatattc tgttaactag
    71101 attaaaatac tatttaatct agttaacagc aaatatattt ttaattagat ttttaccctt
    71161 ttataaagtt agcttctctc ttttcaacga aagctgacat tccttctttt tggtctttag
    71221 ttgaaaaaca aagaccaaat aaattagatt ctatatctat tccagtattt atatcagttt
    71281 gagcaccaag ttgtattgct tctttagagt atctaactgc aagctgagca tttttagcta
    71341 ttatcttagc taatttctca acttcttcta ttaaaatgtc tggctcaacg actctattta
    71401 ctagccctat tttttcagct tcatcagctt ttataacttg acctgtaaag attaattctt
    71461 ttgcttttgc cattccaacc aatcttgtaa gcctttgagt tcctccatat cctggagtta
    71521 ttccaagagt tacttctggc tgaccaaatt tagctttagc agatgcaatt cttatatcac
    71581 atgccattgc aagttcacat cctccaccta aagcaaatcc gtttacagca gctatcacta
    71641 cttttttact attttctatt tctccaaaag cttttgctcc taagatacta aaatctttag
    71701 cagctacagc atctaaatct ttcatgtatg caatatctgc tccagctaca aatgcctttc
    71761 cttcccctgt caatattaca acatcaatag tttcatcatt attaatatct acaaatactt
    71821 cataaagttc ttctaaagtc tttgaattta ttgcattaag ggctttaggt ctattcattt
    71881 tcactgtaca tatatttcca tctacttcaa cagctacatt ctcataaact ttaacatcac
    71941 ttgtactcat aataccctcc ccaagaaatt agttagctaa aacttcacct ttttcttttg
    72001 caacacttaa ctgactgata agttctggaa gcactttatg aacatctcca actataccaa
    72061 catcagcata tttaaatatt ggagcttctg gatttttatt tatagcaact ataaactcag
    72121 catcttccat accagctata tgttgtattg ctccagatat accacatgct atataaaggt
    72181 ctggtcttac agttttacca gtttgaccaa cttgtcttgc tttatctaac caacctgcat
    72241 ctatagtggc acgagagcca gaaacttctc cacctataat ttcagctaat tcataaagta
    72301 tgtctaagtt ttcttttcca cccattccac gtccagcaga aactaatatc ttagcatctt
    72361 ctattttaac ttgtttttta gcttctttta ttacttgtac aacttgaact aatttatcag
    72421 catcattaaa ttctaccttg aaacggttaa ttacagcttc tttagtttca tcaggttcat
    72481 ttttcttcat aacccctggt ctaactgtag acatttgagg tctgaaatct ttacaaacta
    72541 ttgttgccat tatatttcca ccaaaggcag gtcttgtcat taataataat tttgtatctt
    72601 cagctactgc aagacctgta cagtcagcag taagacctgt atgtattcta gcagaaactc
    72661 taggcgctaa atctctacct attgaagttg caccaaataa tacaactata gggtcagctg
    72721 cttttattgc ttcataagct gcttttgtat atggttcagt tgtatacact gctaaagctt
    72781 catcatctac tactattacc tcatctgcac catagtgtgc taatgtatct attaaacctt
    72841 ctaccttact acctaaaagt aatgcagaaa cttttgtatc ataatctttt gctatttctg
    72901 tagcctttcc tagtaattct aaagaaacag tttgaattac attttctctt tgttctatta
    72961 ctactaaaac gttacccatt ctttatcccc ccttaaatta tatgatatac ttctctttta
    73021 atttatctat gataattcca gctgatgttt tcgcatcttc attgtatatt gtaccagctg
    73081 gtttaactga ttttgtaaat gatttaaata cactagttgg agaaccttta agacctaaat
    73141 tagaagggtc aacttctata tcttttacag tccatgtttc tactacatca ttttcgaaag
    73201 catcatatat tcttccaact ttcatgtatc ttggtgtgtt catatcttta agagttgtta
    73261 taaggcatgg cattttaact tttaagtcat ggcaacaatc ttcaaattgt ctttttacta
    73321 atacatattc accttcagtt tttatttctt cagcatatgt tattgatgga agatttaaat
    73381 gttcagctat ttgaggtcca acttgtgcag tatctccatc tatcgcctgt cttccagcta
    73441 ttataatatc aaaatctata ttttttaatg ctcctgctaa tgctgatgaa gttgcccaag
    73501 tatcagcacc tgcaaatgct ctatctgtta ataatatacc tctatctgca cccattgcta
    73561 aagcttcttt taaagccata tctgcttgag gaggtcccat tgttataaca gttacatgag
    73621 cacccatttc ttcttttaat tttatagctt cttctaaacc tgctttatca tcagggttta
    73681 ttatacttgg tactccatct ctaattaaag tacctgtatt aggatctagt ttaacttctg
    73741 ttgtatctgg aacttgtttt atacaaacga ctatattcat aaatccttcc tccatatcta
    73801 ttttaatatc gacctattgt gtatatttta atatttatct attttaataa ttttcctgaa
    73861 ataaccattc tttgaacttc actagttcct tcatatattt cagttatctt agcatctctc
    73921 atcattcttt ctactggata gtcacgagtg tatccatatc ctccatgaag ttgtacagct
    73981 tttgtagtaa cttccatagc tgtttcagct gcaaataatt ttgccattgc tgcttctact
    74041 ccataaggtt ttcctaagtc tttatttata gctgcttgat atacaaggtg tctagccgct
    74101 tgtaccttaa cttccatatc agctaattgg aattgtgtat tttggaattt tgataatggt
    74161 ctaccaaatt gtactctttc ttttacatat ttaacagttt catcaagagc accttgtgct
    74221 aaacctaaag cttgtgcagc tataccaatt ctaccaccat caagagtaga cattgctatc
    74281 ttaaatcctt gaccttcttt tccaagtaaa ttttctttag gtattctgca atcctcaaat
    74341 attaattcac tcgtagctga acctcttata cccattttct tttctttaac tccaaagcta
    74401 aacccaggag ttcctttttc aactataaat gctgatattc ctttgttccc cttagattta
    74461 tcagtcattg ccattactac atatatgtca ccagctattg cgtttgttat aaatattttt
    74521 gagccattaa gtatgtattc atccccgtct aaaacagcag ttgtttgttg gccagacgca
    74581 tctgtaccag cattaggctc agtaagacca aatgctccta atttttctcc acttgctagt
    74641 ggtcttaaga atttttgttt ttgttcttca ttaccatatt gatatatagg ccatgagcca
    74701 agagatgtat gagctgataa tataactcct gtagtaccac aaactctaga caattcttca
    74761 actgccatta tatatcctac agtgtctcca ccttctccac catattcttt tggatatggt
    74821 atacccatca ttcctgcttt tgccattttt tccactgttt cataaggaaa tctttcttct
    74881 tcatcaagtt ctgttgctaa aggtttaact tcattttcag cgaagcttac atatagctct
    74941 ttaagcatct gatatttttt agaatttaaa tccataatag cctcctcagt attttataaa
    75001 tattttattt tcatttatta attaaaatct cggcaacatc tataataagg tatttgaata
    75061 tacgtcgaga aatttataac cttaatcaaa ctacgctcta tatttatatg tgattatact
    75121 aaccaattat gacattaatt taatcataaa ttgttaattt aaaatcaact atacttaaaa
    75181 aaaatctcgc tttttggaat tttgtgaaat ttcaagttag aataagattt ctttcacact
    75241 ggttacagat aaaataattt gtttacgata tttatattag ggtatattaa acattaaata
    75301 ttgtctgtac atctgttttt tagccatgta cagaagattt ttatcgtata aaaattattt
    75361 taatagagct aaataatcgt atttttatct gactattttt tatttttaaa tatcttacat
    75421 ctataatagt atcactcaaa ggataatttt tcaataattt ctaattattt ttttaacaat
    75481 atttattaca ttttttccac ttttttgtca tttttttatt gtttcgaaaa aaaatcagaa
    75541 ttttttaact ttattttgga ttattagttt aaattagatt ttcctcttag atttataaat
    75601 atttacacaa aaaaactacc tctgtataga ttgtatacag aggtagtttt ttactaattc
    75661 ctactatagt ttactatatt tttttaccaa ttcttacaaa catctaccca tttttcacac
    75721 ttagaaaact tctccatttc ttcacatgta agttctattg cagagttact actgccacat
    75781 gcaggaaata ctgtatcaaa tcttttcatt gaatcatcta aatagacttt tatagaattt
    75841 tttaaaccaa atggacagac tccaccaata gcatgtccag taaactccaa gacttcttct
    75901 ggtgtcaaca ttttagcctt acaattaaat tcagccttat acttcttatt atcaatctta
    75961 gcatcaccag ctgtcacaat taaaattgca tcatctccta ttttaaatga gagtgtctta
    76021 gctattcttg ctggaataac cccagctgct tcagctgcaa gttccacagt agcactagat
    76081 tgttcaaatt ctaatatact gtcttccttg ccaaattgtt taaaatactc tcttacatta
    76141 cttattgaca ttaaatatcc ctcctagtct gaacttttat aaatttcata ttattcttaa
    76201 ttatatctat aattgattta tttttcaaat tattccatga tattagcatt ccatgtatca
    76261 ttattttttc caactactag ataaagtcct tttgccattg gaatattgac aatatattct
    76321 cctttatact ctacactcat accagattca ttaaagtacc tataatcaaa atttagtcct
    76381 tttggaatta caaaatcaac ataaccttga ttatttggtt ttattccata tttcagagtt
    76441 ctttcagtta tagaattttt ttcattaaat aaatctacat ttacatatgc agaacactta
    76501 ttcattccct ttttattttc cacacaattt tcaaacttca ttttagcact gtttaattta
    76561 taactatatt tactattatt gcttatttta aatcttacaa tagtaccaac atctctactt
    76621 ctcctgctat caaaatcaaa aacaatttcc tctaaatgat ttatttcata atctttgaca
    76681 cttaccatat ctctatatac taatttattt atagctatga atagtgtaaa aatccccaaa
    76741 ataaaaaatg aaacttttat aaacttcttc atttttagtc tccttatatt ttgtgctatt
    76801 gtgctataat attaatcaaa attttaataa ttaaacttat tcttttttga aatcttaata
    76861 gaaaaataca ttgatattat aaaaattatt attgttaaaa atgtcatact atataaattt
    76921 atatacttag tattagctat atcagttaca taatttgaaa gatatactcc tccaacttta
    76981 ctataattaa aatgctttac ttgatataca atcatactaa tcatcttcaa aagacttcca
    77041 tatacaaagt aaattaaaat acttactata aaaccacaaa ctttcatttt tccatatcca
    77101 aacttaaaaa ttaaaggcag taaaatcgtc aaaattgcta aaaatatatt tacagaaaaa
    77161 aatacatcat ttaaaaccat atttctaaag aacactagtg atgtcaattt acttagtaag
    77221 gacacaatta ttgttacaga aattattaat cctacaccta ataaatattt tgagtaaact
    77281 atatcttcta tactaactgg catactcctt ataaaaatcc ttgaatcatt ttcataatca
    77341 cactcaaacg tatttataaa tatcaaataa ctaacaacaa tacttactgt ataatatgaa
    77401 attgaattaa ggaaaaaata aaacacaaaa aatagcaata tgtactttaa attacggata
    77461 ccatctgaac taatccctat tattaaatct tttcttatta aattgaacat catctcacct
    77521 catttacata ataatacatt agttcttcta tagtaggatt tttgacctta tgcttatcaa
    77581 tttctatcaa taggcttttt actttcaagc tatctccaac tttaacaagt gcctcattat
    77641 aatatttata ctctttcaca ccaactatgc tttctttaat tgcttctaac tctgctttat
    77701 ttgccctaat tatcttatat tcttgttcta ttaattcttt cttaacagta aaaatctgat
    77761 ttcctctatt tataaaaaca agataatcag caattttaat taaattatca gtattatgag
    77821 tagaaattat tacactacta cttccatttt tcacaaaatc ttgtaagtac tgcattattt
    77881 cagttcttac aattgggtct atcccagcag ttggttcatc caaaatcaat aacttagcat
    77941 tgtgactaag tgcacttgat aacataagct tttcagattg acctttagat aaattttcaa
    78001 tatgcatatt ttcttctata tcaaacttat taaggtattc atcaaacctt tctctatcaa
    78061 aattcttata aaataaagaa actatttttc taaattcctt aacctttaaa tgcggataaa
    78121 attgtaaaga atcatataca aaaccaatat tgtttttaaa gcttattgga gaattttcaa
    78181 tagtttcatt acaaagttca atttcacctt catctgcatc aataatcccc attataattt
    78241 ttattaaagt cgtttttccc gaaccatttg gtcctataat cccaacaata tccccttcct
    78301 ttaactctaa gtttatatta tttaacttga attttcctaa tttttttgtg atattcctta
    78361 tgctaaacac atcttcacct cttatgcatt tttttatacc cacctataca tattttaaca
    78421 cattcactac aaaggtcatt tataaattta taaattaaga attataaaac tatgttataa
    78481 tacaaagtat gaagaggtga atatatgaaa agaattataa ctataaatac aaaccaatta
    78541 aataagtccc ttgagactaa agaactctta actagaaaac ttatcaatgc tggctttgaa
    78601 gtgtattcag atatttatcc agacacagag cttattatat caattggtgg tgatggctct
    78661 tttttaagaa cagttagaga tttcgatttt cctgagattc ccataatggg aataaataca
    78721 ggtcatctgg ggttttttcc tgatatttta ccagataaga ttgatagctt tattgaagct
    78781 tatacaaaaa aagattatat tattcaagaa atgtcacttc ttaatgcgga agtatataca
    78841 actactagtg gttctaatat gcttgctgta aatgaggttg ttataagagg agacaagtct
    78901 aggactatac acctaaattt aagccttgat aataaacata tacaaaattt tagtggagat
    78961 ggtatgatta tttctacttc cacaggttca acagcctata actattctgc tggaggtagt
    79021 attgttgata taaatcttga acttatgcag attacacctt tacatccgat aaataccaat
    79081 gcatataggt gctttacatc aagtataata tgttcaaatg aatctgttat aaagattgca
    79141 ccagaatata gatttgaaga ttctgtattg attgtcgttg atggagttga gcacagattt
    79201 agacaaatcg aaaatataaa agttagtata tctgatgcaa aaataaaatt acttagaatg
    79261 tcaaactatg agttttggca tagagtatct gaaaaatttt tatagactgt acttaggagg
    79321 atttttaata tgaaaatagg aataatgagt gatactcatg gtagcttact ttactttgaa
    79381 aaagcgttaa atgtattatc tgattgtgat gttttattac atggtggaga tgtcttatat
    79441 catggaccta gaaatgatat tccagaagga tataatccta aaaaatttat tgagacatta
    79501 aataaattag aaaacattgt gattgtaaaa gggaactgtg atgcagatgt tgaccagatg
    79561 gttatagaac atcctattca gagtccttat gtcatgagcc aatttggtga aattagaatt
    79621 atattgaatc atggttatat agaatcagaa gaagaaatta ttgacaaagc aaagaaaatg
    79681 ggtggtgaca tattagtttt aggacatact catgtaaaaa aactatacat ggatgataat
    79741 ttaatagtaa taaatccagg aagtacttct atcccaaaag atggaagcca ttctgtcgca
    79801 attatagata tcataaaaac tgatgatgag gatgagcttg aacttgatat aaacttaatt
    79861 gacatcaata ctggaaatat aataaatatc aatgattagt aaatttttaa taaaaataaa
    79921 ataagcttta atttattaaa ttcataataa agacaagtaa accattttat ttctatctca
    79981 attagaagtt taagtacaat accttaaact tctaattaaa tagaatctgg tttacttgtt
    80041 ttattatatg ctatattgaa atataa
//
LOCUS       DAMKMI010000003        73141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMKMI010000003 DAMKMI010000000
VERSION     DAMKMI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 73141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.003"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(121..894)
                     /locus_tag="PC429_000185"
     CDS             complement(121..894)
                     /locus_tag="PC429_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF3845714.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC429_000186"
     CDS             complement(894..1280)
                     /locus_tag="PC429_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3845715.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC429_000187"
     CDS             complement(1305..1622)
                     /locus_tag="PC429_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF3845716.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC429_000188"
     CDS             complement(1634..1894)
                     /locus_tag="PC429_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF3845717.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC429_000189"
     CDS             complement(1894..2190)
                     /locus_tag="PC429_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF3845718.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC429_000190"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC429_000190"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF3845719.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC429_000191"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC429_000191"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF3845720.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKRYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC429_000192"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC429_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF3845721.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="PC429_000193"
     CDS             complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="PC429_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF3845722.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8082..9188)
                     /locus_tag="PC429_000194"
     CDS             complement(8082..9188)
                     /locus_tag="PC429_000194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF3845723.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="PC429_000195"
     CDS             complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="PC429_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF3845724.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11408..11545
                     /gene="rpmH"
                     /locus_tag="PC429_000196"
     CDS             11408..11545
                     /gene="rpmH"
                     /locus_tag="PC429_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF3845725.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11623..11967
                     /gene="rnpA"
                     /locus_tag="PC429_000197"
     CDS             11623..11967
                     /gene="rnpA"
                     /locus_tag="PC429_000197"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF3845726.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11999..12250
                     /gene="yidD"
                     /locus_tag="PC429_000198"
     CDS             11999..12250
                     /gene="yidD"
                     /locus_tag="PC429_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF3845727.1"
                     /translation="MNKIIYILKEISKHLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12282..12989
                     /locus_tag="PC429_000199"
     CDS             12282..12989
                     /locus_tag="PC429_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF3845728.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12958..13587
                     /locus_tag="PC429_000200"
     CDS             12958..13587
                     /locus_tag="PC429_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF3845729.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13699..15078
                     /gene="mnmE"
                     /locus_tag="PC429_000201"
     CDS             13699..15078
                     /gene="mnmE"
                     /locus_tag="PC429_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF3845730.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENVIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15176..17068
                     /gene="mnmG"
                     /locus_tag="PC429_000202"
     CDS             15176..17068
                     /gene="mnmG"
                     /locus_tag="PC429_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF3845731.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17061..17780
                     /gene="rsmG"
                     /locus_tag="PC429_000203"
     CDS             17061..17780
                     /gene="rsmG"
                     /locus_tag="PC429_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF3845732.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17943..18728
                     /gene="noc"
                     /locus_tag="PC429_000204"
     CDS             17943..18728
                     /gene="noc"
                     /locus_tag="PC429_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF3845733.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18863..19636
                     /locus_tag="PC429_000205"
     CDS             18863..19636
                     /locus_tag="PC429_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF3845734.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19638..20501
                     /locus_tag="PC429_000206"
     CDS             19638..20501
                     /locus_tag="PC429_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF3845735.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20534..21676
                     /locus_tag="PC429_000207"
     CDS             20534..21676
                     /locus_tag="PC429_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF3845736.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="PC429_000208"
     CDS             complement(21810..22385)
                     /locus_tag="PC429_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF3845737.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="PC429_000209"
     CDS             22645..23937
                     /locus_tag="PC429_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3845738.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="PC429_000210"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="PC429_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF3845739.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="PC429_000211"
     CDS             24717..24980
                     /locus_tag="PC429_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF3845740.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="PC429_000212"
     CDS             24995..25180
                     /locus_tag="PC429_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF3845741.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="PC429_000213"
     CDS             25283..26479
                     /locus_tag="PC429_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3845742.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="PC429_000214"
     CDS             26693..26971
                     /locus_tag="PC429_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF3845743.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="PC429_000215"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="PC429_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3845744.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="PC429_000216"
     CDS             27461..27688
                     /locus_tag="PC429_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF3845745.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="PC429_000217"
     CDS             27831..28151
                     /locus_tag="PC429_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF3845746.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="PC429_000218"
     CDS             28159..29115
                     /locus_tag="PC429_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF3845747.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="PC429_000219"
     CDS             29126..31120
                     /locus_tag="PC429_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF3845748.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="PC429_000220"
     CDS             31121..31570
                     /locus_tag="PC429_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF3845749.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="PC429_000221"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="PC429_000221"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF3845750.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="PC429_000222"
     CDS             33122..33430
                     /locus_tag="PC429_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845751.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="PC429_000223"
     CDS             33512..34801
                     /locus_tag="PC429_000223"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF3845752.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="PC429_000224"
     tRNA            35127..35202
                     /locus_tag="PC429_000224"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="PC429_000225"
     tRNA            35209..35283
                     /locus_tag="PC429_000225"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="PC429_000226"
     tRNA            35288..35363
                     /locus_tag="PC429_000226"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="PC429_000227"
     tRNA            35397..35471
                     /locus_tag="PC429_000227"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="PC429_000228"
     CDS             complement(35854..36846)
                     /locus_tag="PC429_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3845753.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="PC429_000229"
     CDS             complement(36855..37913)
                     /locus_tag="PC429_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF3845754.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="PC429_000230"
     CDS             38038..39501
                     /locus_tag="PC429_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF3845755.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="PC429_000231"
     CDS             39603..40400
                     /locus_tag="PC429_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3845756.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="PC429_000232"
     CDS             40413..42515
                     /locus_tag="PC429_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF3845757.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="PC429_000233"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="PC429_000233"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF3845758.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43211..43522
                     /locus_tag="PC429_000234"
     CDS             43211..43522
                     /locus_tag="PC429_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3845759.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43570..43887
                     /locus_tag="PC429_000235"
     CDS             43570..43887
                     /locus_tag="PC429_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3845760.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44100..44801
                     /locus_tag="PC429_000236"
     CDS             44100..44801
                     /locus_tag="PC429_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3845761.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45025..46326
                     /locus_tag="PC429_000237"
     CDS             45025..46326
                     /locus_tag="PC429_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3845762.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46463..47557
                     /locus_tag="PC429_000238"
     CDS             46463..47557
                     /locus_tag="PC429_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF3845763.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            47559..48692
                     /locus_tag="PC429_000239"
     CDS             47559..48692
                     /locus_tag="PC429_000239"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF3845764.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48710..49819
                     /locus_tag="PC429_000240"
     CDS             48710..49819
                     /locus_tag="PC429_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF3845765.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49884..50624
                     /locus_tag="PC429_000241"
     CDS             49884..50624
                     /locus_tag="PC429_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF3845766.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGYVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50629..51621
                     /locus_tag="PC429_000242"
     CDS             50629..51621
                     /locus_tag="PC429_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF3845767.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVLICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51809..52690)
                     /locus_tag="PC429_000243"
     CDS             complement(51809..52690)
                     /locus_tag="PC429_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3845768.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52963..54351
                     /locus_tag="PC429_000244"
     CDS             52963..54351
                     /locus_tag="PC429_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845769.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54379..55332
                     /locus_tag="PC429_000245"
     CDS             54379..55332
                     /locus_tag="PC429_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF3845770.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            55392..55616
                     /locus_tag="PC429_000246"
     CDS             55392..55616
                     /locus_tag="PC429_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845771.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55609..56121
                     /locus_tag="PC429_000247"
     CDS             55609..56121
                     /locus_tag="PC429_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF3845772.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQEDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56153..57007
                     /locus_tag="PC429_000248"
     CDS             56153..57007
                     /locus_tag="PC429_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF3845773.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57541..58335
                     /locus_tag="PC429_000249"
     CDS             57541..58335
                     /locus_tag="PC429_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3845774.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58439..59245
                     /locus_tag="PC429_000250"
     CDS             58439..59245
                     /locus_tag="PC429_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3845775.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SITVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINEDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59307..59828
                     /locus_tag="PC429_000251"
     CDS             59307..59828
                     /locus_tag="PC429_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF3845776.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60243..62330
                     /locus_tag="PC429_000252"
     CDS             60243..62330
                     /locus_tag="PC429_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3845777.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62345..62632
                     /locus_tag="PC429_000253"
     CDS             62345..62632
                     /locus_tag="PC429_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3845778.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62659..63930
                     /locus_tag="PC429_000254"
     CDS             62659..63930
                     /locus_tag="PC429_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF3845779.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63988..64587
                     /locus_tag="PC429_000255"
     CDS             63988..64587
                     /locus_tag="PC429_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3845780.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            64592..65497
                     /gene="dapA"
                     /locus_tag="PC429_000256"
     CDS             64592..65497
                     /gene="dapA"
                     /locus_tag="PC429_000256"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF3845781.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPSKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65603..66235
                     /locus_tag="PC429_000257"
     CDS             65603..66235
                     /locus_tag="PC429_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF3845782.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            66713..69220
                     /locus_tag="PC429_000258"
     CDS             66713..69220
                     /locus_tag="PC429_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3845783.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69562..71724
                     /locus_tag="PC429_000259"
     CDS             69562..71724
                     /locus_tag="PC429_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF3845784.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNETTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRALNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71729..72346
                     /locus_tag="PC429_000260"
     CDS             71729..72346
                     /locus_tag="PC429_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3845785.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNKKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            72535..72879
                     /locus_tag="PC429_000261"
     CDS             72535..72879
                     /locus_tag="PC429_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF3845786.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
BASE COUNT        27956 a         8683 c        12178 g        24324 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ctttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa atacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttaacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat ctctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 acaatacttt tattttagtt ccttctattt caactatatc tcctgatttt atcttttttc
     7801 ctcttcttaa ttctatttca tcatttactg tcactaaacc ttcttgaatt aaaaattttg
     7861 catgacctcc tgttgatgca atctctgcta atttaagaaa ttgatccaac tttatatatt
     7921 ctgattctat agttatttca gtcataatag ttcctccttg tcactatttt aaacaaattc
     7981 ctattgatac actaaaagta tattatacca taaatatgct tataaaagga gttgtctaag
     8041 attagaaaaa ttttctacaa aagacaactc cttcatgtct gctatatatt tgatgatatt
     8101 cttactggaa gcagtaagta aatataatta acatcatctg ttggtttaat tatacaagga
     8161 tttacatttg tagtaaattc tataaatatt tcctcattat ctatattttt caaaccttcg
     8221 ataaaatatc tagaattgaa agctatatct aggtaatctc catctaaatc tatttcaact
     8281 tcttcataca catttccttt ttctgtatta gaagtaattg ccattacttt atctcttata
     8341 gataacttta taagattatt tttttctgat tgagacaata aagacgctct ctctatacta
     8401 tttaaaagtt ccttagtatt taatttaact ctgctgttat gttctcttgg caataatttt
     8461 ttataatcaa taaaatctcc ttcaagcaat cttgtaataa ttttcgtatc attaattata
     8521 aaaatagcat ttttttcgtt aaatccaact ttaacattat cctctccaga aagcaaacta
     8581 tttacatcta taagtgtctt tccaggaatt atgactttta tattttcagt taaactatca
     8641 accgaacaac tttttacagc taatctatat ccatcaattg ctactaaatt taagtttcta
     8701 tctacaattt ctaaaagttc tcccattaaa acaggttttg tttggtcctg tgagatagca
     8761 aatacagttt gtttaatcat attttttagg atttcttgag gtatgctata taaatcttct
     8821 tcatttaatt ctggtaattt aggaaattct tttgctgcat aacctttaat tttaaatctt
     8881 gaatttacac aatttatgta aatattattt tctgaatcag tttctatctc aacaaaggaa
     8941 tctggtaatt ttctaattat atctccaaat agccttgcat ctacaacaac atcacctttt
     9001 tctatgattt ctgcttgtac ataagtttct attcctattt cagcatcata tccagtaagt
     9061 tttaattgac cttcttctgt agatataagt atacctttta ataattctat tgttgttttt
     9121 ccattaatag ctttttgagc aattccaatt ctatttgcta ggattttttg attacaaatt
     9181 attttcaatg tggataacct cctctggctt tttatatata aaaataagtt aaaatcatag
     9241 tagtaatagt agtaggtgtt gttgataatg ttaataagta tgtataacat tataaataca
     9301 aagatattaa catgtgtata agatgttaat atctttgtta ttttagactt gataatttgt
     9361 taataatttt tgttcaaaaa aagtattaac aggttattca agagatataa acatacaatt
     9421 gtgtataaat tatcccttca aatctgatat aattttgtct attttagtct taatttcttc
     9481 actttcttct atatccttag atactttgtc atgagcatga attactgttg tatggtctct
     9541 acccccaaac tcttcaccta ttttaggaag ggataaatca gtaagctcgc gcgttaaata
     9601 catagctatc tgtcttggat aagctattga acgtgttctt tttttagaat taaaatcttc
     9661 catctttaaa ttgaatacag aagatacttt ttcttttatt ctgagaacat ttatttcttc
     9721 atttttagaa gttgttataa tatcttttaa agcttctgta gcaagatcaa aagatattac
     9781 cctattgcta agagaagaat aagcaaccac tctagttaat gcaccttcta gttcccttat
     9841 gtttgactta atattttttg ctatatatga cataacctca tttggcacat ctatacgttc
     9901 tagttgagcc ttttttctaa gtatagcaat tcttgtctca aagtcaggtg cttgaatatc
     9961 agttataagt cccatttcaa atctcgatct tagcctatct tctagtgtag gtatgtcttt
    10021 tggcggtcta tcactagata taattatttg tttgtttgct tcatgaagag tattgaatgt
    10081 atggaaaaac tcttcttgag ttctttcttt tcctgctata aattgaatat cgtcgattag
    10141 taatacatct acgtttctat atttattcct aaattcttcg tttttatcgt cttttataga
    10201 gtttataagt tcattagtaa atttttctga agaaacgtaa actacttttg agtctttttt
    10261 ttgactaaca atatgatgac ctatagcatg cattaggtga gtcttaccta atccaactcc
    10321 tccatacaaa aaaagtgggt tataagcttt agctggagat tctgcaactg caacgcatgc
    10381 agcatgtgca aatctattac tattaccaat aacgaatgta tcaaatgtgt attttggatt
    10441 tagattgggg taaagaactc tataatttaa atctgtgcta tcagaattta aatcagcaac
    10501 ctctttttcg ctcaatacaa atttaatatt atattttttt aatgacagtt gactaatagc
    10561 atcttcaatt aaatgtaaat atctattctc taagatatct ttattaaaat cactaggagc
    10621 cagtaagatt aaatcattaa gatgtatttt taatggtacg atgtttttaa aaaaggtatt
    10681 aaaacttact gaagttaagt caccttttat taattgtagg gttttgtccc ataaagaaac
    10741 tatatccata atataaacct ccgaacagaa taattaatat tttccacaaa gttattaaca
    10801 gaaaagcaaa aatatatata aattttacag aataactaaa tttatggata atggtgttga
    10861 taaattaata atagtataaa ttattaaaaa aatatgaaaa gatataaaca atatgtagat
    10921 aaagttataa ataacataaa catacaataa atggtgatta taactagagt attgtcagta
    10981 atcttaaaag ttatacacaa aagtatccac aagctgtgaa taatttctaa taagttgata
    11041 acatttatgc acattatcaa caagtatatt tataatatca aaatatatgt caaacttcaa
    11101 tgatttgtaa ctaatttatc cacaatatta actaattgtt gataaaaata taaacatgtt
    11161 aaaattactt tatttgtgta tataattgaa aaaaataatt tagaataaaa ttaaatagat
    11221 ttttactagt attttcaata aaaaaaatac ataatatata tatggtagat tttgtgatat
    11281 aagcgtccca aaaactcgtt gacaacagta gtaaaagtat ttataattat atgtgttatg
    11341 tttactttga gggtaaagtt attattttta taaacggaaa ataaaatctg aaaggcggtg
    11401 caaaataatg agtaaaagaa cttatcaacc aaaaaagaga caaagaagta aagaacatgg
    11461 tttcagaaaa agaatgaaga cgtctaacgg aagaaacgtg ttaaagagaa gaagagctaa
    11521 aggaagaaat agattaactc attaattaca aaagggccgc tgcaaaggtg gcctttttgt
    11581 gtaaataatt acaattcata gctgtgttta tggaggttaa ctatggactt taataggact
    11641 aaagggttga aaaaagactc tgattttaga aaagtatata aacacggcaa atcttttgca
    11701 aacaaatatt tagtaatata tatacttaaa aataaatcag attatagtag agtaggtatt
    11761 tctgtttcaa aaaaagtagg aaaagctatt actaggaata gagtaagaag attaataaaa
    11821 gaagcctaca gattaaatat tgatgaaaaa attaagcctg gatatgatat agtatttata
    11881 gcaagggtat ctagtaagga tgctactttt aaagacatag acaagtctat aaaaaattta
    11941 gttaaaagaa cggatatttc tatttaattg tatgctataa atatggaggt taatgacttt
    12001 gaataaaata atttatatat taaaggaaat aagtaagcat ttatcgaatt tatgtattta
    12061 tttagtgaga ttttatcaaa agtatatatc tcccttaaag ggacctacct gtagatttta
    12121 tccaacttgc tcacaatatt caatagaggc gtttaagaaa tatggatttg taaaaggtat
    12181 gtatttaacg ataagaagac ttttaaaatg tcatcctttt catccaggag gttacgaccc
    12241 tctaaaataa aagagagtaa ctattaggag gtataaaaaa attgaatata ataggaaatt
    12301 tcttaggatt gattcttaag gtaatatttg aatttgttaa tcattatggt atttctataa
    12361 tactttttac gatacttgta aagattattt tattaccatt aacaattaag cagactaagt
    12421 caacaaaggc aatgcaagat atacaacctc gtataaaaga gatacaagaa aaatataaaa
    12481 ataagccaga aaaacaaaat gaagaaattg ttaagttata tggagaagct aaaattaatc
    12541 cactatctgg ttgtttacct ttattaatac agtttcctat attaatagga ttatttagcg
    12601 ttttgagaga accagttgct catggcgttt ttgcaaacaa agctgcattt ttagcagctg
    12661 acaatgggtt tttatggata aaaagcttaa catcaccaga ttatgtatta gcagtatttt
    12721 caggagcaag tgcatatgtc atgcaaaaag taatgacacc aaaagatcag ttacaaggct
    12781 ctatgaaagt aatgacatat gtaatggcag gtatgtcttt ctattgggga tttatattcc
    12841 cagcaggact tacattgtac tggacagtaa gtaacttatt ctcaatagct caatattact
    12901 taataatgaa tcctttaaaa gctaaactgg ctgctaactc taaggaggaa aaagttaatg
    12961 agaaaaagcc taaaattaat aaaaagtaag agtaaagagg aagctataag taaagctata
    13021 gcagagttga atttaaaagc agaggatata gaagtagaaa tacttgaaaa tccaagtaaa
    13081 gggtttttag gttttatagg tgctaaagat ggtacttatg aaatttttgt tatcgagagg
    13141 gaagtagatg tagctaaaaa ttttatagag gtaatgttga aaaatgcaaa tgttgatgcc
    13201 aaagtcaatg tatctcaaaa agataattta atcatggttg atattgaagg caaagaagct
    13261 gcaagtttaa ttggtagaag aggggaaact ttagattcta ttcaattttt gactggatta
    13321 gctcttaata aaataaataa agattctcat acaagagtac ttgtagatat agaaaattat
    13381 agatcaaaac gagaagaatc tttaataaga tatgctaata aagttgctag agaagtagcg
    13441 aaaacaagaa aaactaaaaa attagattat atgaatccat atgagagaag aataatacat
    13501 tcagctcttc aaaatgacaa atatgtaatt acatatagtg aaggaaccga tccatataga
    13561 agattagtaa ttgagtgtaa aagataaagt caacctccta aacattaggg ggttttattt
    13621 tttaatatta ttaaatcatt aatggttggt taaatgtaaa tctatatgta aaaaattatg
    13681 aattaagagg tgataaattt gtttatagat gatacaattg cagcaatagc tacagcacct
    13741 ggagaaggtg gtataggtat acttagaata agtggagaaa aagctttgaa ggttgcagaa
    13801 gaaatcttta aatctatgtc tggaaagagt attgaagaat acaataagag aacattaata
    13861 tatggaaata tagtggataa tgaaaatgta attgatgaag ttttattggc atatatgaaa
    13921 ggaccaaatt cctatacagg agaagatgtt atagaaatta attgccatgg tggatttatt
    13981 tcggtaaaga agatattaga attaatacta tcaaaagatg ttagattagc agaggctggt
    14041 gagtttacta aaagagcatt cttaaatgga agaatagatt tatctcaagc agaggctgta
    14101 attgatgtta taaaagccaa aactgatata gctcatgaag ttgctcaaaa tcaattagag
    14161 ggttctttat caaagaagat aagagaattg agagataaag tcactgagat attagctcat
    14221 gtagaagttg ctatagatta tcctgaggaa gatattgagc atataacata tcaaactctt
    14281 aaagaaaaaa ctgatgaact taaaaaagac attaaaaaat tatatgatac agctgaaagt
    14341 ggaaaaatac ttagagaagg cttaaagacg gtaattgttg ggaaaccaaa tgtaggtaaa
    14401 tcttcactac ttaattctat tttaggagag aatagagcaa tagttactga tataccagga
    14461 actactagag atgttataga agagtttgta aacataaaag gtataccttt aaaaattgtt
    14521 gacacagcag gtataagaga tactgatgat attgttgaaa aaataggtgt tgaaaagtct
    14581 aaagaatcat ttactagtgc agatttgata gtaatggttt tagatgcatc tagaaagttg
    14641 tcagaagaag atatagagat attagaaaaa cttaaggata aacaaacaat agtgctgtta
    14701 aataagaatg atttgaaaca agagattgaa gaagaaaaaa tacttaaata tgtagaaaat
    14761 aatagtataa taaaaatatc agctttacaa caagaaggaa tagaagaatt gcaagataaa
    14821 atagagtcta tggtatataa aggtagtatc aaaaataatt ctagtttagt agtaacaaat
    14881 tcaagacata aagatgcact tagtaaagca tataaatctg caacagatgc tttaattgct
    14941 ttggaacaaa gtatgccatt tgactttgta gaagtcgatt taaaaaatat atgggactat
    15001 cttggatata ttaatggtga tacagtaaca gaggatttac tagataatat attccataat
    15061 ttctgtattg gtaagtaatg tacaaaagta aacttgtttt tattatagag atttttaaaa
    15121 aatttgaagt gtttctcatg aaacattaat tttgaaaaga aaggaattat aaatgataaa
    15181 gtttgaagca ggaaaatatg atgttatagt agtcggagca ggacatgctg gatgtgaagc
    15241 tgctttggct actgcaagaa tgggatataa aactttgata ataacaatgt ctttggattc
    15301 catagcatta atgccttgta atccatcaat tggagggact ggtaaaggtc aattagttaa
    15361 agaaatagat gccttaggcg gtcagatggg attaaatata gacaagacat atatacaaag
    15421 tagaatgtta aatactgcca aaggtccagc ggtacactct ttaagagcac aagcagataa
    15481 atttaaatat catgaagaaa tgaaaaagac attagaagat gaaccaaatc ttgatatagc
    15541 tatggatgaa gttgtagaaa tacttcatga aggaaatgtt gtaataggag ttggaactaa
    15601 actaggatgt tcatttaaat ctaaagctgt aatattggcc actggagttt atctgaactc
    15661 aaagatatat atgggtgaag ttgcatttta tgaaggtcct aatgcattag ggtatgcaaa
    15721 atatcttaca gatagcttag ttgagcttgg ccttagaatg agaagattta agacgggaac
    15781 tccagcaaga gttcatagag atagtataga cttctctgta atgtctttac aagagggtga
    15841 tgaaaaagta actccattct catttatgaa tgaaaatata gaaaaaaaac aagaaccttg
    15901 ttatttaaca agaactacag aagaaactca aaaagttata ctggataatt taaaaaggtc
    15961 tgctatgtat agtggagtaa tagaaagtac tggtccaaga tactgtcctt ctatagaaga
    16021 taaagtagta agatttagtg ataaaacatc acatcaatta tttatagaac cagaagggtt
    16081 aaatactaaa gaaatgtata tacaaggaat ttcaactagt ttaccttttg aagttcaatt
    16141 agatatgtac aaaactataa aaggacttga aaattgtaag ataatgagac ctgcatatgc
    16201 tattgaatat gattgtgttg acccaactca actaaaaata agtttggaaa taaagggtgt
    16261 tgagaactta tttagtgcag ggcaatttaa tggaacttct ggatacgagg aagcagcagc
    16321 tcaagggctt atggctggta taaatgcagt tagaaagata gaaggaaaag agccatttgt
    16381 attagaccgt tcagaagctt atataggtgt cttattagat gaccttgtta ctaaaggaac
    16441 taacgagcct tacagaatga tgacatcaag agcagaatat cgactatatt taagacaaga
    16501 taatgcagat atgagattaa ctcaaaaagg ttatgatata ggattagtta aaaaagatag
    16561 atatgaaagg ttcctaaata aaaaggctgc tgtagagaaa gaatttgaaa gactaaaaaa
    16621 tgaaagagtt acaccaaaag aagtaaatag tttgttggaa gaaaaaggag caacaccaat
    16681 aaaggttgga atatctctat atgaattttt aaagagacca gaggttactt acgaattgct
    16741 tgaagaatta gggaaaggcg ctggagaaga tgtttcaaga gaagttaaag aacagtgtgt
    16801 aataataact aaatatgaag gttatataga gaaacaattg aaacaaatag accaatttaa
    16861 aaaacttgag aataaaaagt tggatgagaa aataaattat tcatctatag aaggtcttag
    16921 attagaagct agacagaaat tagatgatat aaaacctatt tcaatagggc aagcatctcg
    16981 tatatctggt gtttctccag ctgatatatc agttttactt atatacctag aacaaatcag
    17041 aagaactaga ggtggcaaag gtgaataata tagaacttct gaaaaacggg attgaaggtt
    17101 ttaatataga tacgaatgac agtatgttag aaaaatttaa aatctataga gagatattag
    17161 ttgaatggaa taaaaagatg aatcttactg gaatagaaga tgaaaaagaa gtgtatataa
    17221 aacatttttt agactctatt tcagctgtaa aaaatggata tataaaaaat gggatgtcta
    17281 taatagatgt tggtacaggt gctggttttc ctggaatacc attgaaaata tgtttagata
    17341 gcttagaact tactttgtta gattcgttaa ataaaagaat aaacttttta gaggaagtta
    17401 gtagagtact agagttagat aatattacat ttatacatgg aagagctgaa gattttggta
    17461 aaaatgaaga ctatagggaa aaatatgatg ttgctacggc aagagctgtt gctggattac
    17521 cgattcttat ggaattttgt gtaccatttg taaaagttgg agggtatttt atttgcttaa
    17581 aaggtccgaa tgctaattta gagctagaag aatcacaaaa ggctattgat gttttaggat
    17641 tggagtatat tgaaaaaata gatgtagagt taccagaggt agatttaaat cataatatat
    17701 tagtttttaa aaaagtgatg gaaacaccag ttaagtaccc tagaaaagct ggtaaaccag
    17761 ccaaaaatcc tattaagtaa tgaaaaattt attattggta agcttaatta tttctcagga
    17821 aataattaag cttactgtaa aaaaacaaaa ataaaggaat tattaaagta gatgcagaaa
    17881 taaatttgta tgtacatata gagaacaaaa ataatttata tctaaaataa gagaggtatg
    17941 ttatggaaga taaaagagtt atggagatac ctatagaaga tgtagttcca aatccatatc
    18001 aaccaagaaa aatattttca caagtttcat tggaggaatt gagcaattct ataaaggtat
    18061 atggtataat tcaacctata acagttagag caaaagatgg caaatatgaa ctaattgcag
    18121 gagaaagaag attaagagca gctaaattag cagagcttaa aaccatacca gcaattataa
    18181 ataatatgaa cgatgaatct tctgctgttc tagcgttatt agaaaatttg caacgagaag
    18241 acttaaattt cattgaagaa gcaattggtt atgaaaactt aataaaagaa catgctttta
    18301 ctcaacagca attagcagaa aaattgggca aaaatcaatc tactgtagca aataagctta
    18361 gaatattaaa gcttccaaac gatataaaaa tgaagctaat agagaataat ttaacagaaa
    18421 gacatgctag agcatttctt aaactaccaa gtgaagattt aatgcaaagt gtgttagaca
    18481 aagtcataaa aaatgaattg actgttaaaa aaacagaaaa attgatacaa gatgtattag
    18541 aagaaactaa agttcaagaa gagcctgata aaaaacagaa tataaagggt gctatgagta
    18601 taagaattta cataaatact ataaagcagg cctttgatgc tatatcaaat acaggaattg
    18661 atgctaagta taatgaaata gataagggag attatatgga agttgttgta aaaatcccta
    18721 aaaaatagag attactgctt accagtaatc tttttttgta atataagcct tgtaatataa
    18781 attgatttat agacattatt tatacttaaa aaacacctat tcaacattta aaattaattt
    18841 aattcttaaa ggggggaaca tcatgggcaa agttatagct gtattcaatc aaaagggtgg
    18901 agtcgggaaa actacaacaa atgtaaattt aagtgctagc ttaggaactt tgggaaagaa
    18961 aatattagta ttagatttag acccacaagg aaatacaact agtggatatg gtattaataa
    19021 aaacgaagta gaaaatacaa tctatgaaat tatgttagat gggttacata ttaaagaagc
    19081 aataataagt actgaatttg agaatgtaga tgtagttcca tcagctactg aactatcagg
    19141 tgcagagata gagcttacat ctaaaactaa tagagagtat attttaaaaa attccataaa
    19201 agcggtaata gatgaatatg attatatatt tttagattgt ccaccctcac tggggatgct
    19261 tacaataaat tgtttaactg ctgtagatag tgttttaatt ccaatacaat gtgaatatta
    19321 tgctttagaa ggtgttagtc aattaatgga gactataaaa ttagtaaaat caagattaaa
    19381 tgctgatata gaaattcaag gtgttgtttt aagtatgttt gatggaagag ctaatttatc
    19441 aatacaagta gttgaagaag ttaagaaata ctttaaaggt agtgtatata caactttaat
    19501 tccaagaaat gtaagacttg cagaagctcc aagtcatgga aaacctgtaa tttattatga
    19561 taaaagatgt agagggtctg tagcttattt ggaactagca gaagaattta tagatttaga
    19621 agaggaggaa tggtagtatg gagaataagt ctaaaagaag taatagatta ggtagaggtc
    19681 ttagtgcatt gataccagaa attaaaggag aaacttcaga aaaagaaata gtcaacatag
    19741 atatagacaa aatatatcca aacgaagttc aacctagaaa gcaatttgat gaagaaaaga
    19801 taaaagtatt gtcggattct ataaaaaatt atggtgtttt acagccaata gtggttaaaa
    19861 tggatgaaaa taataaatat atgataatag ctggagaaag aagatttaga gcatcaaaac
    19921 ttgcaaataa aaatcaaata cctgctataa ttaaagatat agatatgaaa gatataatgg
    19981 agatagcctt aattgaaaac ttacagaggg aagatttaaa ttcaatagaa gaggctctag
    20041 catataagag tttaatagag cattacaatg taactcagga agaaatatca gaagctgttg
    20101 ggaagagtag acctcatata actaatacac taagactttt aaatcttgga caagatgtta
    20161 tagaaatgat agacagtggt agaattacag caggccatgg taaggcttta ttgagaattg
    20221 cagataaaga tttgcaactt caaatagcga aaaaaataga agaagaagaa ctttctgtaa
    20281 gagaagtcga aaatatagct aaaaaaatat ctgaaaataa acaagaagaa cctaaaaaat
    20341 ctaaacctaa ggatgtattt attttagatg ttgaagataa acttagaaat atatttggaa
    20401 caaaggtgaa tatttctaaa ggaaagaaaa aaggtaaaat agagatagaa tactataatg
    20461 atgatgattt aaatagtatt gtatcaatgc ttcttgaata gaacaaaaat ataaaacaga
    20521 gaggaagatt gttatgattt atttagataa tgctgcaact acatatccaa aaccagaaag
    20581 agtgtataat gctgttttag attgtatgaa aaattattgt gcaaatccag gtagagcagg
    20641 ccataaactt gctatgagag ctgctaggga gatatatgat acaagagaga atatagctaa
    20701 gttgttcaat gtaagtaatc ctatgaatat agtatttaca tctaatgcaa cagattcttt
    20761 aaatttagcg ataaaaggtg tattacaaga aggagaccat gtaataacaa cgtctatgga
    20821 gcataattca gtaataagac ctataaaagc attggaaaaa aggggtattg aaaatactgt
    20881 agtaaaatgc gattatgagg gatttttaga ttatgaagat ttagaaaaat caataaagaa
    20941 taatactaaa ttgattgtaa caacacatgc gtctaatgta tgtggaactt taatagatat
    21001 aaaaaaagtt ggagaaatag ctaaaaaaca taatatacta tttttagtag atgcatcaca
    21061 aacagcaggt gtttatgata tagacgtaaa tgaatgtaat atagatatgt tggcaatgcc
    21121 aggtcataaa tgcttatttg gacctcaagg aacaggtata ttatatgtaa gagaagggtt
    21181 gaacttaaat atattaaaag aaggtggaac aggtagtaaa tcagaagaaa tagttcaacc
    21241 agagttattc ccagataaat atgaatcagg aactcataat actccaggta tagcaggtct
    21301 taaccaaggt atactattta tatttgaaag aggaatcaac aatataagac aacatgaaga
    21361 agaattatgt caatatatga tagacaaatt agaggaagta ccagatataa agatatatgg
    21421 accaaaggac agtaaaaaaa gagcatcagt aatagctcta aatataggag atatggattc
    21481 tggagaggtt actttcttat tagatagtga ttataatata gcgactagat caggaataca
    21541 ttgttcacca ctagcacata ccactttagg tactttaaaa caaggtgcag taaggttcag
    21601 tataggatat ttcaacacaa aagatgagat agataaagca gtggaagctt tgaaaaaaat
    21661 ttcaaaaaat aaatagcatg agatttaata atatagaaat aaaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggttc ctaagaacct
    21781 ctaatctatt tgataatagt ttcaagaaat tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 ggtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagagtaa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa atttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtatttat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttatg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattaaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaacca ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagaatct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaata taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagagcaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt atagggccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttga cttacattga ttttttatta agttaaagct
    34921 acttatacat attctaactg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 ataatattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taaggtacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag attctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aggtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcacttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacttt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tcttcattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag aaacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacataga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagctga aaacgttaga gattttgcat ttatactaag tagtaaattt
    38821 gatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagtga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcttt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aatagtgaat gtaagtaaat tttgtgcaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aaaaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttaa
    40261 gcaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aactgctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtggtaatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac cgttacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt taagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gagcttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctttagggct ttctgatgag gttttaggaa gggctgatta taggttatcc
    42961 ttttctaaga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gtttttgtta
    43081 atttgacact taataatatt aaaataacaa aaaagtattg aaagtatatt gcatttaagg
    43141 tagaattaaa taaaatatta taacattata atgtttatat ggttgttaat aaaaatttgg
    43201 aggaaaatat atgataaaaa taatgttggc ctgttcagca ggaatgtcta caagtttatt
    43261 agttactaaa atggaaagtg cagctaaaga aaatggtata gagtcgcaaa tttgggcaat
    43321 accagaatca actatacaaa atgaaataga aaaatgtgat gtattattac taggaccaca
    43381 agttagatat gtattaccta aggctcaaga aattgctaag ccatataata taccagtaga
    43441 agttataaat atgatgcact atggaacagt taatggagaa gctgttttaa atagagcgat
    43501 agaacttaac aacactaagt aatataaatt ataatgtatt tttaatatat aaattgggag
    43561 aatcaaagga tggatgaaaa gactatagaa atatctttta atataatagg ttatgcaggt
    43621 gaaggtaaag gattagcttt tgaagcaata aaagaggcta agaatggaaa tatagaaaaa
    43681 gcaaaagaat tattaaaaga gtccagggaa gttataaata aagcacatag atatcaaaca
    43741 gaattaatac aaaacgaagc tagtggaaac aggactgaaa taagtgtcat acttatacat
    43801 gcacaggacc acttaatgaa tggcatgaat tttcaacagt tagctgagga aatcgttgat
    43861 ttacatttga aattacaaga taaataaaaa ttaaagataa taaattaaga gagttcttaa
    43921 aagatacatt atgtgtagca cactaattgt tagattattg attcaatatt tagtgagtaa
    43981 tacattagga ttattttgag gactcttttt atatttagtt taaagttaat aaatatttat
    44041 tattaataat ataagattat atgttataat attataacat ataatttaag aggtgaatca
    44101 tgttaagaaa tcaaccatta tatatacaag ttgtagatat attaaagcaa aaaatattaa
    44161 ataacgaata taaaattgat gaaaatattc caacagaaag ggagttagag caagagtttg
    44221 gagtcagtaa aattactatt agaaaagcta ttgagatatt agaatcagaa gggtatgtag
    44281 agaaaaaaag tggaaaaggg acaaaagtaa taagtaatag tctttttaat aagctatcaa
    44341 aagcagagtc tttttcaagt atacttttat ctaaaggtca taagttatct aaaaagacta
    44401 tttcaattga aaaaataata aatgatacaa attcacaagt atttagtata tttggagaaa
    44461 aatgtagcaa gattactaga atgtattact tagatagtaa gccatatata ttttttactc
    44521 attatattcc ttttgatgaa aatctaactg ttgaaagttt taataatgaa agttctttat
    44581 atatgtactt atataggaga aatataaata taactagatt tgaagatgaa ttttttatag
    44641 aagaatcaga actagaggta tcaaaggctc taaatctagc taaagacata ttattaggaa
    44701 gaaaaagaaa gacctatgat gagaatgata atatagttga gctatcattt gcaagatata
    44761 atacagaagt acaaaactat gtaattaaat acgaaatata ataagaatcc tgagtttatt
    44821 gaaatgataa tatgctcagg atttttgttt agttgtaaaa ataaaaaata ttataacatt
    44881 ataatgttta caactaaaca ttataatgtt ataatatttt taataaacaa gtgatattat
    44941 caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa taaaggataa
    45001 tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga gcgcaagatt
    45061 atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag agcaggtata
    45121 atagcaacat taccactaac aatagtgggg tcatttttca caatattatt aaacatacct
    45181 attgatgctt atatggattt tatagctcca tttaaagcaa tattagatat accatttaga
    45241 tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc acttgctaaa
    45301 agctacgatt tagatacaac aggtgttggt atgcttgcag ttatggcatt tttagtttct
    45361 acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc tggtagatgg
    45421 atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac atcattaatt
    45481 acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat gccagcagga
    45541 gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt aattttaatg
    45601 ttattctggg taataagaca tattttagga tttgatataa gtgttgcact aagcgctatg
    45661 ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggtt actgacagta
    45721 ttcctaataa ctttattctg ggtacttgga atacatggtc cagctataat ggggccagta
    45781 ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac agaaggagta
    45841 tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta tgtatggata
    45901 gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa gtcacaatat
    45961 ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa tgaacctata
    46021 atatttggag caccaatagt tatgaatcca atactaggga taccatttat aattggacct
    46081 ataataatga caattctatc atatgttctt actattacag gggttatacc tatgatggta
    46141 gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac taactggagt
    46201 atattagcag gtatcttagt attaatcaac tttgtgatat catttgctgt ttattatcca
    46261 ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat gagtgaaaat
    46321 ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa aaacttataa
    46381 gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat attttaaata
    46441 atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat atccagagca
    46501 tagtaacatg gaagatgata aggcatatat aaaaatggct tcaaaatacg gatttaaaag
    46561 aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa aacattttaa
    46621 agagattata acctatgcaa gagaattagg atttgaagta atccttgata tagctccaaa
    46681 catatttgat atattacaaa tatcttatga tgatttaaca ttttttagtg agttaaatgc
    46741 tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga tttctttcaa
    46801 tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact tggataatat
    46861 aattacttac aagccaaatg taaataatat atatggttgt cacaattttt atcctcaaag
    46921 gggtacaggc ttgccatatg atttttttgt aagatgttca aatagattta aaaagtatgg
    46981 aataagaact gctgcttttg ttaattcaaa atatgcaaca atcggacctt ggaatataaa
    47041 tgatggactg tgtacgcttg aaatgcatag aaacttagat attgctacag caactaagca
    47101 cttattcgca acaggattga ttgacgatgt aattgtaggg aatgcatatg caagtgaaga
    47161 tgaattaaaa agaatgtctg aagtaaataa atatcaagtt gtatttaatg tagtaactga
    47221 ggaaaaaact tctgaagtag aaaagacaat aatggcaaat gagcaacact ttagaagagg
    47281 ggatataacc aaccaagtag caaggtctac agaagtaaga aaaaaatatg ctaaagaaaa
    47341 tttccctaag catgataata cgaccatctt taaaagagga gatgtagtga ttggtaatga
    47401 tgactttgga aagtataaag gggaattaca gttgatactt caagattgtg cagatgaaag
    47461 aaaaaataag gtaggtagaa taaaggaaga cgaaataatg cttttagact ttattgaacc
    47521 ttggagtaaa tttaaactta ttttagaggg agagtaaagt gaagatagac atattaataa
    47581 aaggtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac gataaaataa
    47641 tagaagtaaa aaatcagata gatgattcta agtatcaggc aaatcaaatt ataaacctag
    47701 atggagaaaa atttattagt gctggttgga tagatataca tacgcattgc tacgaaagac
    47761 tagaccttta taaggattat ccagatgagg ttggaataaa aagcggtgtt actactgtag
    47821 tagatgcagg aacaacagga gctttagata taggtaaatt ttatgaagat actaaaaaat
    47881 ataaaacaaa tgtatatgct ttaataaata ttgctaagca gggaataaca tctcaagatg
    47941 aactttcaag tatgatgaat attgatgaat atgaacttaa aagagcagtt aaaaaataca
    48001 aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt tcaaatgatg
    48061 ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct atgatggttc
    48121 actttgggag ttctcctcca acgatagagg atatatttga ctatatggaa aaaggagata
    48181 tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat gaggttaaaa
    48241 aagaaatcat agaagctaga gaaagaggta taatccttga tgtaggtcat ggaaccgaaa
    48301 gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca gacactataa
    48361 gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta tctacaacaa
    48421 tggaaaagtt tatatatatg ggctattcac ttgaagatat aatagataaa gttactaaaa
    48481 atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga tatgatgctg
    48541 atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct ttgaataagt
    48601 cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga gaatatttaa
    48661 atataggtaa gtctatagga gagaatgagt agaagggaga aactattgta tgagtagtat
    48721 atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga ctatactagg
    48781 agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta aaaatttctt
    48841 tataatagaa gaacttataa ataaaacagg tgaatatata gcaaatttat tagggtgtga
    48901 aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg gagcaatgat
    48961 agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac aagttaaaag
    49021 ggaaataata attccaaagg ggcataatgt tgattatgga gttccagtag aagtaatgat
    49081 aagacttgga ggaggagaag ttgttgaggc cggctatgct aatatgtgta acaaggaaca
    49141 tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat ctcatcattg
    49201 tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa gttgctaaag aatataattt
    49261 acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg aattgggagc
    49321 agacctagtg atatacagtg gtgctaaggc tatagagggt cctagctcag ggttagtaat
    49381 aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag gaagagctat
    49441 gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat atataagtaa
    49501 tgataaagta actttaaaag aaatggagga caagctaaat ccatttatag acaagataaa
    49561 tactataaag ggagtgagtg ctagtataac tagagattca gcaggtagag aaatacttcg
    49621 tggagaaatt gactttaatg aagatataat aaataagtca acacaacaaa ttataaatga
    49681 acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg aaggcaagat
    49741 tgagattgat ataagaagtg taactggaag tgatttagat actatttata ataggataaa
    49801 aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta ataaattaaa
    49861 ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa aatagagttt
    49921 gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat gcttgtgatg
    49981 ggtatgtatt gataggagta ctatcaaaga attacagtac tgtagaagca gctattgaag
    50041 atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtggggctt ggagctggag
    50101 acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca caacatataa
    50161 atcaagtgtt tacaggtgtg gctacgtcta gagcattgct tggtcaaaat gatagtataa
    50221 taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc acaggtccat
    50281 taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct atgatgaaag
    50341 atatgggagg aagctcaatt aagttttttc caatgggagg gcttaaaaca aaggatgaat
    50401 atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca acagggggta
    50461 tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt gtagaaaaag
    50521 ttatacctca tgtttataca tcaataattg ataaagaaac aggtgaaacg aaagtggaag
    50581 atataggagt actttataaa ataatgaaag aattattggg atagatttat gaatatatta
    50641 gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt aacacaaacc
    50701 aatgaactaa attatataat tacaggcaca ggattaaata tattatctgg acttaaaaac
    50761 tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg taaagcatca
    50821 agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacatatgg agggaatcat
    50881 ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt gacttatttg
    50941 aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat tgataaatgt
    51001 ttagaagaaa tggatatagt ccttatttgt ggtattgcta tgcaacttac agattcaatt
    51061 aaagagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa agtattcttt
    51121 gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt agtaaatgat
    51181 tataagaaaa tactaccata ttgtcatggt ctatttgcta gttatagaga tataaataag
    51241 atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa aaaagagtat
    51301 gatataggta ttgtagctgg aactatgaga agatttgatg atatgaataa tagatttata
    51361 aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa gttagaaata
    51421 tatgacaggg ttggtgccgg agatggatat gtagcaggaa ttatatattc atatttggaa
    51481 aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt agctcataca
    51541 acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagacttat gaacaatgaa
    51601 aaaatagata tattgagata atacatttgt atataataaa atttttatct tttttagaca
    51661 cacttttgac ttgtttatat ttgttaaaca gagttatttt taatgattat aaatattaaa
    51721 ttaaaagtta aataataaaa tagtagaaaa tagcattaac tatagaaaaa atggttaatg
    51781 ctatttttat cgttaatatt gattaatttt atattataga aatgaacttt tctaatgctg
    51841 gatttgtatt ttttttattc catacaacta caacttcaat tctattttct acttctttta
    51901 taggtactat tgcaatatca cgttctatca ttgtatctgt aacacaaaat gaaggtaaaa
    51961 tggatattcc catgttagaa gataccaatg attcaatagt attaagatta tttgaacgtt
    52021 gtaccacatt aggtacaaac cctgcttcac gatatctatt ctgtatcatt gacttttgtc
    52081 catactcatc ccctgtttct tttacaaaaa taaatttatc atgttgtaat tcaattaaat
    52141 cgatagaatc ctcattgtaa agtggatgac caggaggtaa aagagctacc aaagaatatc
    52201 tttctacagt tttatattca aaatctttta atggttgatt ttctggatta ataacaaaag
    52261 cgaggtccat agaattattt tttactaagt ttaaaagttc ggttatttca ttagtacaaa
    52321 tatccataga tatattaggg tatgtattag aaaattcttt taaatattgt tgaagtttgt
    52381 ttttttcata tcctattaag gttccaatgc tcaaagttcc aaaaaaacca tgtgcaaatt
    52441 gttgtgtttt tgcaatagca tcattgatat tttttattat agtacgtgcc tcagataaaa
    52501 aaacttttcc agctggtgtt agttcaacat gacgtttact tcttgtaaat agagttacat
    52561 ttatttgttc ttctaaagct tttatttgct gtgtaacagc agtttgagaa atataaaatt
    52621 tttctgctgt tttggtaaaa cttaaatttt ctgctacaga tataaaatat tctaattgtt
    52681 tagtattcaa ataatcaccc ctatttaaac ttgattgttt gtaaacctta tttatctata
    52741 attaaagctt attatagtat aagaaaattt acttttaaag aatattagaa aatatttgta
    52801 taaaaatata tgttttaagt atatctttac attagatttt ataaatttat taataagaat
    52861 aacttatata ttccaatgaa aacgatattg gatttaagta agtttgtctc ataaaatgaa
    52921 agtatatcaa ttgataatac tttaaataag aagggagaaa aaatggaaga taaattttat
    52981 gcaaaaggca acggaaataa cggatatatt aaaaatcttg aagtttgttc ctttaataac
    53041 ttagatggaa cttgtggaat gtttcaaatg gctctgtaca aaagagatga aaaatactat
    53101 ttatatggat gctgttttgg aggaaataaa aaaaatggag taatgattag cgatattaca
    53161 gacccttata atccacaatt tataaaacat tttcaaatgt tagaccctaa agagtatcct
    53221 acaacaacaa ctcccaaaat tcaaatagca gatgatttaa tgatagtagc aatgagttgt
    53281 ggaagtggac caggagcact tgttgaccaa gctaaattag caaatattaa gtgtgaagca
    53341 ggaattagaa tatatagttt aaaagaagac cctttaaatc ctaagttttt aggatattgg
    53401 gattgtggct taaagcatgt aatgggtgtt catagattca tgtacaatgg tggaagatat
    53461 gtacatttat caagtgattg tgttggcttt gaaggtctga tttatagggt catagatata
    53521 ataaatccta ctaatccagt ggaaataggt aaatggtgga gaccagacca atatgcagat
    53581 ggatatccaa atagaacttt tgatgcagga gcacctcatt gcccagaatt tatggataaa
    53641 ggatggcttc atggacctcc atttgtaaga gacggaaaag catattgtgg ttatggagga
    53701 gctggtttag ttgtattaga tgttgaagat ttaacaagac caagatgctt aggtgaattg
    53761 ccatttatgc ctgcattttc tagtagactt gcaggtgcaa gaactcatac agcattacca
    53821 ttgccaggaa gagatttagt cgttgttcaa aatgagggag aaagattcca gttctttaaa
    53881 ccagataaca ttacagatgt tcaagctatg aataatatac atatggttga tgttagtgac
    53941 ccaacaaaac caacattaat tgctcaattt ccatatcctg aagttccaaa agatttccct
    54001 tatcctaact ttaatgttgc aggattagga aaaccagggc catttggtcc acataatctt
    54061 catgaaccaa tggataataa accatggtta gagcaaagag gagatagagt atattgctgt
    54121 tatttccatg cagggctaag ggtttatgat gtatcagacc catattatat caaagagcta
    54181 gcatatttta taccaccaaa tccaaataaa acaccagaag aatcttattt cccaggattc
    54241 ccaggaccac gcttggcagt aacagaagat cttatcgttg atgatagagg ctacatcatc
    54301 atagatgctt tagatgatgg attctatata ttaaaaatga aagaagatta aataaatact
    54361 aaagagggag agttgaaaat gaaaatacta ggtatttctt ttggaacaaa aaatggaaat
    54421 aatgacacaa tgtgtaaagt tgcacttaaa ggtgccaaag aagcaggggc tgaagtagaa
    54481 tttattcaaa tgtcatctat taacattaaa cattgtactg gatgttgtgc gtgtgttaaa
    54541 actttattat ctgggaaagg tagtatgtgt gtattaaaag atgattttga atggttgctt
    54601 gacaaaatga aagatgcaga tggtatagta gtttcagacc ctatttttga agaaggggca
    54661 tcaggattat tccatactat aatggaccgt tttggaccca gagcagatag aggaaataac
    54721 attataggaa caaaagttgc agaagctata ggaggaaaaa ttccagattc aagaatgctt
    54781 aatgataaag taatatcatt tatgggaata ggaggttctg actggggtac aagagttcaa
    54841 tgtgaacatg ctatgctagc tctcatacca atgtggaaag ttatagataa tgcttggttc
    54901 ccatgggcaa aggaattagt aatggatgat acaaggcttg ttcaagttca tcaaattggt
    54961 ttaaatatag ttgaagcagc aaaagatttt gaaaaagctt catatcaagg agaagaaggt
    55021 gtatgtccac attgccacaa tagattattc taccttgagc caggtactaa aaaggcaatt
    55081 tgtgctctat gtggtatagt tggagagtta gatactataa gtggaaaaac tatatttagt
    55141 tttccagaag agcaattagg tcatgctcat gatactttac caggtaagtt tatacatggt
    55201 gatgatatta agaaaatgga aggtcattat gcagaagttc gtaaaacaca agaatttaaa
    55261 gaaagaaaaa catcttataa ttttatagag acattaacta aagaaaaaca agctggagtt
    55321 tcattaggat aataattaaa atactaagac tcttttttaa gggatgttag atgttatagg
    55381 aggcttattg tatgaatgaa aagccaagtt ttaaacaaat tgaagttatt ttagcagtta
    55441 tttttttggt acttacatat tacaaggtta gattaactcc agcatttatt gttatattta
    55501 taccactata tttaagttat tttgcagtat ctatgattgt aaatcaaata gcaattaagc
    55561 tattggagat aaggcaagct agggaagcta ctaattacaa tataggaaat ggatagaaag
    55621 aaaaaagaaa tattaacact gtctatgggg attgctttac tacctccttt atgggcagta
    55681 ttagcacctt atataggaat aaaaactgga gctgtagcac taatctgtgc aggattatat
    55741 gtaactaatg gtaataaaca agaagatgga ttaaaaataa tgtttggttt ttggtgtgga
    55801 gatttatggg cagtgttagc cattttaatt atgggatata tgaatttcaa tcaaaaccta
    55861 gaattatttt taacacttag tatattaggt ttttttgcag ttgtaattgc atccttattt
    55921 gaaaaaataa ttttcctacc atcatggctt tgtggttggg ctataggtct aactattatg
    55981 acaggtgaaa atattattaa tttacaagat atttgtattc aaattgctgt agctatggca
    56041 gtaggagtat ggtacgtggg tgcaggtgta gatttgttta caatatatat tttgagtaaa
    56101 gataggaaaa agggaaattg aaagcatgtg aatatacttt gattatagga ggatggctat
    56161 gggttttgaa tttaaaataa tgagaagttt aatatatgta ggacttgcca aggaagaata
    56221 tagacctaag ctaatggact ggttatatcg tcaccatatt ccagatagta ttagcacttt
    56281 tggaccatat tgtactaaat atgcctttta tcaagcatat cctacaccaa atgaaggtga
    56341 gcgttttggt gcacgtaaga tgcaactaac agaacattat tggcttgtag atgaacatat
    56401 gcctgagatg gcaaatagaa ttatgacaga atatatgcct atggatgttc tacgttggca
    56461 agggtgtata ccagatgtag aaaataaaag ggttcatgaa aatgcagaaa gtggagatgc
    56521 aggacgtgca gtaggtggag ataatggatg tccaccattt atatttgcct ttgttccaat
    56581 aaactgggaa gaagacttta gaggaaaagg acgtactgta caagatggac caaactatcg
    56641 ttggcaattt atgattaagt atccagatgg tatctctaaa gaagaaggag aaaaatggtt
    56701 ctatgatgag gtagtgccat actttacaaa ctgttgctat gttaatcgtt ttgtcagtag
    56761 taaaataatg attaattatg gagcaactgc ttttgaccgt gtgtcagaac tatggtttga
    56821 aggggaagaa gaatggtata aagctgtggt tgaagagaca aagtcgttta ttaaaaaacc
    56881 agaatgggca caagaagagg agttcccata tttaaaacca caattcaata tcgcatcagt
    56941 attcttaggt gatatagcaa ctatggatgc atactcacag tatcgtggat atataccaat
    57001 gagataaaaa ttatagtttg tgataagtag agaaggcatt tactcttact ggtagaagaa
    57061 gacttattgt atccttaaaa gtaggtgaat atatagtttt atatttaaat ttatataaaa
    57121 ctaagactaa taaatatgaa acaatagaat tagaataatt tgataatatt tgttaaaaga
    57181 gcatttagaa tgtgtttaaa gattaagata ttccttttat ggtagcagat agttgaaata
    57241 ataaaactgt tatcatgaaa ggaatatttt tatgtatagg aaatttttaa aaataaaagt
    57301 taaatatgtt taagaacatc ttaaagtgaa agaatcattg ttagaaatat gtagacaata
    57361 tagtatttat aacgtatcat attaacaagt ttacacttgg attatgaaat aaaaagagta
    57421 attctaaagg ttgtaaatac tatgctgact ttgatacttc aaagtttagg tgattttgta
    57481 attgttaata gaaatacgtt taatgagcat aaagaattta tctaataata ggagaatata
    57541 atgtatacaa ttggacaagt atctaaattt ttaggaatat ctagagatac actaaaattt
    57601 tatgaagaca aaggattagt aaatcctaag aaaaatgatg aaaatggata tagaatatat
    57661 aatcaggttg atatctatga tattgcaaca ataaactttt atagagaaat tgatatagaa
    57721 attaaaaaga ttcaagaaat aagaaaaagt aaaagtataa acaatttaga actcttacta
    57781 gaagagaagg aacaaatcat actaaaagag atagaatata aaaagcttct cttaaaaaag
    57841 gttcagttag tcaaagaaga ttgtgacaaa atcaaaacat attttggaaa atacacaata
    57901 aaagaaatga agcctttgga agtaaaaggt aaaataaaac atgttactaa ctatgaggaa
    57961 tatgaagcct taaaagagaa tacaaacaac ttaaaaaaag cagttacatt aactgattta
    58021 agaagagtaa taaattttaa tgaagaaggt ataatagaag aaaaatttat aattgtaagg
    58081 gaagtagatg actacaacaa aatcatagaa ggtgaagttc tatctcatcc caattgtatt
    58141 tatactatta ttgaagatgg cagatggtca actggaggag aaaatattga taataaagtg
    58201 gaagacagat taagagaaat agcaatagaa aatgaatgtg aattacttgg aatagtctat
    58261 gttaatatat tgcttactac ttatgaagaa gggattgagc gtatattttt agagatttat
    58321 gcacctataa aataatataa aagcattgac ctgggactta ctcataggtt taaactcatg
    58381 atataagcag ttataactta tatagcaatt aaatattaat ttgaaatgag gattacttat
    58441 gagaacagag aaatcattaa aaaggcctat agccagtttt attattctaa caaatataat
    58501 ttttttacca ctttttttat tggtagtagt gactataatg ttaaaacttc caacagtgat
    58561 atctgacatt gcgttgtgta tatcagcgtg gtcatccact tttgcattta tgatattatt
    58621 taaaaaaatt tatccaggta agaaattttt agtacatgta aaagatagat ttagaaataa
    58681 acttaaattt tcaacagtat ctataataat tagtatacaa gtcctaatag ctgtagtagt
    58741 tatatttata ttagcatcta aaaatcatgg aataatgcct aactttacag tttcatcatg
    58801 gggagcattt atatatttat ttttaaaaaa tttatttgca ggatcattag gtgaggaatt
    58861 aggttggaga ggatttgttc aaaatcatct tcagaaaaga cattctcctt taaaagcatc
    58921 cataacagta ggtttttggt gggggatgtg gcatttacca atatggttta caacaggatt
    58981 caaaggactt gatttaataa aatatattat attttttatg attgcaatta tatcaatcag
    59041 tattataatg acaacttttt ataatctaaa taaaactttg ataataccta ttgcaataca
    59101 tcaactattt aattttttaa tagggataat taatgaagat ttaatagagc ttataaatta
    59161 ctatgcaatg ttatatttaa tatttgcaat tgcattaata gttataaatc caggaaatgt
    59221 attatataaa aatattagag agtaatatat atctttatgg taagatataa agatattatt
    59281 agaagaacat ccaaggagga ataaacatgg ttttactgat tgttgatgca caaaagttaa
    59341 taacaaacga gaggttatat aaatttaatg aatttgtagt aaatgtagaa aatttaattg
    59401 atacagctag aaaaaataat atagaagtaa tatatgtacg tcatgatgat ggagttgaaa
    59461 atgaattgac taaaggtaaa aatggctttg aaatatacga aaaatttaaa ccatgtaatg
    59521 gagaaaaaat atttgataaa aaagtaaata gtgcttttaa agaaactggt ctattagaat
    59581 acctaacaaa taatggtgaa aaagatatta ttattgctgg tctccaaaca gattattgta
    59641 ttgatgccac tataaaatgt ggatttgaac atggatttaa tataatagtt ccagcttatt
    59701 caaatacaac tgttgacaat ttttttatgt cagcagaaca atcatataaa tactacaatg
    59761 aatttatatg gaatgaaaga tatgcgaagt gtatttcatt agaggaaaca cttagaaaaa
    59821 tgaaataaaa atagagtatg aattgtatat gtacataagt tgatttcata aaaaaggatg
    59881 ttaaattcca agttaaaaat tagattaaat atattatatt atagataagg cacatcaatt
    59941 ttattttaaa ttaatgtgct ttttaattgt agtaatttat ttataatcaa tatgattttt
    60001 attatttaaa tcaatttttc ttgagtttct ttgtctaaag aaactttttt tatttataaa
    60061 atctacagtt atattatagt atctatttta gatgagttta atttttgtat caatctaaaa
    60121 caaaagttat acttaaatga aattgaaaac gataaaattc tcaaataaaa taagtattgt
    60181 ggaggtgatg aaagaatcca atgataatat gaagactaat cttaataaga aagtaggggt
    60241 taatggtagt ggacaaagaa attgtatcaa ttctccagat tttattaaat agcacattga
    60301 ttacaactaa tggattacaa gaggagtcta attcatcaaa gagacaaatt acttatagaa
    60361 taaataaaat caatgacatg cttaaagtaa aaaatgtacc acttatatgt ttaagagccg
    60421 acaaagatat tatcgttaga aaagaaacaa aaaaagcaat taaagaaatt ttagaaaaaa
    60481 actattctaa gaatacttat tattttagta agacagaaag acttttatat atgtatttaa
    60541 tgctttttat taacatggag gatatttcaa ttaatcattt tataaattct ataaaagtaa
    60601 gtaggtctac tgtaaatctt gatttcaagg atttgatacc agagcttgaa aagaagaaca
    60661 ttaaagttaa gaacaatcgt ataaatggat attacatagt tggcaatgag atggaaatta
    60721 ggagagtact tattaaaaac attatagaaa ctttgtctaa tgagaaaagt tcaagagtat
    60781 ttgatatttt tattaaagaa tataatttag atagttttga agaggctaaa aaaattatat
    60841 taaagctaat aaaaaaatat gaaattactt ttgttgaaga tagattaata gaatttatat
    60901 atatatttat ctttctaaaa gcaaggatgt attcaactag aatagttact caggaaaatg
    60961 tagatattcc taatatttca gttatgagtt caatgaagga gtataaattt gctaaggaat
    61021 tattagaagt atatgggagt gaagataaga taaagcctca caatataatg tatattagtg
    61081 cttggatttt aggtatatca gtaggaaatt atgaagaaga tacagaagat aaggatgtaa
    61141 tttcaaaaat tgtaaataaa atgatgacaa agtttggata tttaagcgga gtttactata
    61201 taagccaaga aaaaatattt aaacaattat attctcattt tagacctgcc tactatagac
    61261 ttttatttaa attacctatc tataatccga tttgtgaaaa agtaaaagaa gaatataaat
    61321 tagtttatag aattgtaagt aatgcaatga aagagttttg cggattattt ggagaagaaa
    61381 ttcaggaaga agagttagca tatcttacaa tgcactttgc aacacttttt tcaaataaaa
    61441 aagaatttga tggttttaga aaaagaatag cattaatagt ttgttcaaat ggcgttggta
    61501 gttcagctat tttacatgca gaacttacaa acttatttcc tgacttacac tttataccaa
    61561 tgtcggaatc agttggatta gaaaatataa gtgaacctgt agatattgta tttactacta
    61621 attataacgc agagggttta aaaacagatg taccagtaat taaagtaagc cctgttatga
    61681 ccacaaaaga aaaatataag attacacgtg aagtgtatat tcaattaggt gatatttttt
    61741 taagacaacc taaaatagat gaggttatgg atataataaa aaaatatgca aatgtaacct
    61801 ctgaaagtat tctttcaagt gagctattag cttattttac acagatagag aattttacat
    61861 ctaaggaagg agaggggcct atgctaagtg agattactaa tgaaacacta gtaaaaataa
    61921 aggtaaaagc taagaattgg gaggaagcta ttagacagtc agcttctgta ttagttgaaa
    61981 ataacaaagt aactgaaggg tatgtagatg ctatgattaa ctcagcaaaa gcatctggac
    62041 catacatagt aattacaaaa catgttgcac ttccacatgc tagaccagaa gctgggtcaa
    62101 aagagattgc aataggcatt gctactttag atagtcctat tgagtttggg aataacgaaa
    62161 acgaccctgt aaaatatgta ttttgtctaa gtgcaataga caataatagc catttacgtg
    62221 ctatgtctga gctagttgaa ctattggaag aagatgagtt ttttaaggtt ttagatagtg
    62281 ctaaagaagc taaagaaatt attgatttta taaaaacaca cgaattataa aggaggattt
    62341 aattatgtat aaaattttaa tcgcatgtag agcaggagtt ggctcaagtt taatgttaaa
    62401 aataaagact caacaaatta ttaaggaaaa taactttcca attgaagtag agcatggttc
    62461 attggattca ctaaatggat tcaatggaga tgctgtagaa acattaattg atgttgctga
    62521 agaattaaaa aataaaaatt tgaagtttga tataattggg attcacaata ttgttgaccg
    62581 taatgaaata aagacagcac tagaaaaatt ccttgcttca aaagagggat aaatttaaga
    62641 tgataaggag gaatatttat ggagtttatt attagtttgc tgagtacacc tgctgttttg
    62701 cttggcgtag ttgcatgtat aggattgatt cttcaaaaga aatcagctgt tgaagtattt
    62761 acaggctctt caaaaacact tatcggtttt ttgatatttg gtattggtgc ctctgctatg
    62821 acaggagcac ttcaaaattt caataaactg ttccaacatg gctttggaat tactggtgtt
    62881 atagcttcac cagaagcagc tacagcactt gcacaaggta catatggttt tgcagtttca
    62941 tgtacattaa ttcttggatt catattaaat ttagtatttg cacgtattac aaagtttaag
    63001 aatattttct ttacaacagg tcatagtttg ttcttttctt gtgtattagt tttaattatg
    63061 aaagcacatg gatttgataa tacaatcact attttaatag gtggaactat cttagggttt
    63121 atgtctgctg cactacctca attctgtcaa ccatttatgc gtgaattaac aggtggcgac
    63181 gaacaagcta ttggtcactt taacatgatt ggttatggtt tatcaggtta tattgggcgt
    63241 ttattttcaa aacataaaga tgatacaaca gagactatag aatttcctaa gtggttatca
    63301 atattcagag acttcttaat gggtctatcc atagttatgt taatcttatt ttatattgca
    63361 acattaaaag ctggtcaagc atttactcaa gaaattgctg gttcaacaca ttggttggtt
    63421 tttccgctta tagaggcatt tacatttgtg gcaggtatgt caatattgat gtcaggtgtt
    63481 cgtatgttct tagctgaaat aactgcagca tttgtaatca tttcagaaaa gtatattcca
    63541 ggttcacgtc ctgcgctaga tgttccaaca gtatttccat atgcaccaaa tgcagttatt
    63601 attgggttta tatcagcata tgctgctggt ctattagcaa ttgctattat ggcatcattt
    63661 cctagtatat ttcctgtagt aattattcca gctgctcaca tatgtttctt ctcaggaggt
    63721 acagctgcaa tatttggcaa tacttcagga ggatggagag gggctattgc tgggtcattt
    63781 gttgtggggt tattacttgc atttttacca gttatactat atccagttta tggcactctt
    63841 ggtatagaag gagcaacttt ccctaatatt gattacaatg tagttggttc gatattacat
    63901 aatattttac aatttataaa acctatttaa actaaattat ataaaagtat agtcatagtt
    63961 actatgattt caaggaggat acatcttatg gaaaatacat tatctgaatt tttagataat
    64021 atatctaatg aattgtcaga atttattgcc tcaatagatg agacatctat aaataaagca
    64081 tgcgaattaa ttcttgaagc tgaaaaaaat cacggacgtg tacatgtgac tggtattggc
    64141 aaaccaggac atgtatcggg atatatttca tcattgcttt catctactgg aacgtcagca
    64201 tatatattgc atggtacaga agcagtacat gggtcaagtg gtcaagtagt tgaaggagat
    64261 gttgttattg caatatcaaa tagtggtgaa actcaagagt taaaagcaac tcttaagact
    64321 ctaaaagtaa atggagcaaa gataattggt gtaagtggta atgagagttc atttttaaaa
    64381 aatatttctg atatattcct gtttgcaggt gttaaacagg aaggagattg tcttaataaa
    64441 gctcctcgtg cttcaatttt agctgaaaca ataatattac aatcattaag tgttgtatta
    64501 caatatgcta aaggtcttaa tacacagcag tatttgaagt ggcatccagc aggaagtctt
    64561 ggaaagtcta ttagagaggg aatataattt tatgaaatat aaagggatta tatgtgcaat
    64621 gataacacca tttgatgaaa atcaaaatat taattcacaa gcaacatgtc aattaattga
    64681 ttatttgatt gaaaagggaa tttatggatt atttattttg ggcactaatg gtgaatgtca
    64741 tgtattaaca gatgatgaaa aagttgaatt tgcaaagatt gttattaatc atacaaataa
    64801 tcgtgtacct gtttttgttg gaacaggggg taattcaact cgtgaagttg tagatttatc
    64861 taagagaatg gaagcgattg gagcaagtgc attatctatt attacaccat actttgttac
    64921 accaacacaa caggaattaa tcttacatta caaaactatt gctaattcag ttaatttacc
    64981 tattataatg tataatatgc caagtaagac tggaattaat attgaaccag agtcagtacg
    65041 tgaattatca aagataaaga atattgttgg aattaaggat agtagtggta aacttgacaa
    65101 catgaaagct tatattgaag caacgaagaa tgagaatttt agtgtgtttt caggctcaga
    65161 ttcacttatt ctggatagtc taaaagctgg tagtcaagga gctgttgcag caacaacaaa
    65221 cttcctcaca gagattggta tctctatcta tgaaaacttt atgaaagggg atttggaaga
    65281 agctcaaaaa gctcaaagta gtatagaaga acttcgacgt atactgaaac ttggaacaat
    65341 tccatctgtt atgaagaaaa ctattgtctt gaatggaatt aatgttggaa ctgcacgact
    65401 tcctgtaact gaaccaactg gggaagtact tgaagaaatt aaacgagtag ttgaaaatta
    65461 taggacaata cttaataaaa gtagtgagaa tagataatat aaatagggaa attacactgg
    65521 tatataatta atatatatat tataaaacat gactaaaatt aatgaataag taaagtggtt
    65581 tgatgagtat agaggtgata atatgaaaaa gatgaatata accaaaagaa tgtcagaatg
    65641 tggggctctt gcaattgtaa gagaagaaaa tcttaaccgt gcatgtgaaa ttgctgaagg
    65701 atgtataaaa ggtggtataa cagttataga aatgagttat accctaaata atgctggaga
    65761 aataattcaa gggcttaata aaaagtatgg tgaaacactt tgtgtgggag caggaacagt
    65821 tttggatagt gaaacagctc gtcatgctat attacatgga gctcaattta ttattgcacc
    65881 aaattacaat gaaggtgtag cgaagatttg caatagatat cagattcctt atgctccagg
    65941 atgtacatca ttgactgagg cagtagacgc actaagttta ggagcaactt ttataaaagc
    66001 tttcccaata tcagatttct atggacctaa actagtaaag gtatttaaga caccaatacc
    66061 tgatatgcca gttttagcaa gtggagggat taatattgaa aacctacata tatggttaga
    66121 aaatggagtt gatgtatgtg gatttggtgg tttattaact aaaggttctg ttgaagatat
    66181 tgctaaaaat gctaaaaaga ttcgtgaaat cattaaaaat ataagaaaaa tttaacatag
    66241 ggctgtatca tagtatctta taattactat gatacagcct ttttaatatt attcaaatta
    66301 aaaacagttt acatttctat tatattacat ataatttcag taattttatc ttaatttttc
    66361 tatctggaaa aaaatgattt aaaacttaag ttatgatata atttcattaa aagaaggcga
    66421 tttttagtaa atatgagaaa ataatgtgtt tttaggggaa aagttaaata cagatttaag
    66481 tgaacaagaa tcgataatta agatattaaa tggtgaatag ggcatcaaaa aaattactaa
    66541 attaaaagtt ttagagggag aatcttagca agtgtgtgtg aaaattcaat ttcaaacaaa
    66601 aaataattgt aaatgaaatc aatttataca cgttgaagat tcacttttat cattatatta
    66661 aaaagcagag tgaattaaaa gtgagtctaa agataggagg ataattaaaa caatgaaaga
    66721 agacattagg ttacatcgaa tgtgtaaaaa agaacaagca acaaagaatg aagttatatc
    66781 agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa cattttttag atgattcatt
    66841 tacaataata tggggaaata catcattcta tcaattatta ggatatacag aagaagaatt
    66901 tttagttaaa ttttctagtt taaaagatta ttatgaacag aatctttatc attttgaatc
    66961 tatgaaaaat cattttttag aggcttatca tcatggcgaa aagagtatag aatatcaaat
    67021 ttgcatttca gcaaataatg gagaccctgt ttgggtaata atgactggta tattaattga
    67081 atcacaagat aatgagaaat caatggtcta tatattgtat tcaaaaatta atgagttaaa
    67141 aataaagcaa gaagaactat ctgatatgga aaaagagagt acagagaatt ttaaatggat
    67201 gatgtcagag tatgctggga atgtttatat tagcgatatg gatacatatg aattactata
    67261 cttaaataag cactcatgca atactttaca ggcatcagca aatgaactta ttggaagaaa
    67321 atgttatgag gtaattcaag gtagaacttc gccatgtccg ttttgtacaa attcgtattt
    67381 aaaaaaagat gacatatatg aatgggaatt ttataataaa aaactgaaac gaacatttat
    67441 aattaaagac cgtatgttga attggcaagg gcgaagagtt cgtattgaat tatcttatga
    67501 tatgtatagt gctgaatata aattagcaaa aaaagatcag gaaagagaat caattttgaa
    67561 aactattcca gcaggaatgg ttagattaga tgcaagagat tatcttacta tactttggtg
    67621 taatgatata tttttgaata tgattgaata tacaaaggaa cagtttgcag aggaacttaa
    67681 taatcaatgt ggatatttat tttctgaaga ttataaacgt agtaagaaac tggcacaaga
    67741 tttgaaggag tctggggata atgttgtatt tgaagcaaaa atatatacac gttcaaggga
    67801 agaacgtata tggactgtta cattgtgtta tatcagtggg gaagacagtt gggatggaat
    67861 accatctttt tatagtatag gtattgacat aacaaaggaa agaaaacaga ttgagaaatt
    67921 acagcatata tcagagaaag atgcattaac aggaatttat aatcgtgctg agactgaaag
    67981 gcagattaag aaatattttg aaaaaaattt aaatgttatg ggtgcactgt ttatgattga
    68041 tacagataat tttaaacaga ttaatgatac agaagggcat atgattggag atatagtact
    68101 tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac agtgatgttg ttggaagaat
    68161 aggtggagat gagtttacaa tttttatgaa gaatatttcc tcagttaaag atgcagaaaa
    68221 gaaagcagaa gaactattgt atatgttccg tcatctattt cagaaagaaa aaagttttct
    68281 aaaagtaact tgtagcatag gaatagctat atatccaaag gatggtacta cattcaagga
    68341 aatatatgca agagctgata aagccctgta tcaagcaaaa aatatgggaa aaaataatta
    68401 tgtaatctac aaccaagatt attttaaaga attagaagaa tttgattgtt cttctcttgg
    68461 tacagttatt gattcagaaa aacaatacgc tgaatatcct gataatctta cacgttatat
    68521 attccgtatg ctttatcaga ctgatgatat agaccaggcg attaatatga tacttgaggt
    68581 tgttgggaaa cagtttgatg taagtcgtgt ttatattttt gaaaatacag aagatggtag
    68641 atatacttct aatacttatg aatggtgtaa tgaaggaatc tcttctcaaa tggcttattt
    68701 gcaaaacagc aattatcaag actatgagga ttatgaaaag ctttttgagg atgaattagt
    68761 tttttattgt cgagatatcc atacattaag tcctaaacta gaagaattgt tttctagtca
    68821 aggtatccac tctacacttc aatgtgcata taaagaagat agagttttca gtggatttgt
    68881 tggatttgat gaatgcacag gactacgtct atggtcacaa gaggaaataa gtacattatc
    68941 attgatttct caaattatat caatgttttt acaacgtaaa aaaaataata aacttaatca
    69001 acagattcaa caataccaga atattttaga taatattgac cagtgtattt gtgtgattaa
    69061 taaagaaaat aattttttat tatatgtaaa taaaaaattt aaaaatacat attctaaatt
    69121 ccaaattgga caaccttatt atcttgattc tatagatatt cataagatat ccattatatg
    69181 gaataaaaaa gaagcatacc tatgtactag cacgatttaa tggtaaagta aagtttatat
    69241 tggacttaaa ttaaatttcg ttttaaataa gatattattt tagttgctta ctgaaaagac
    69301 tagaatctat attaatgaat tatactaatt ttataccact attaaaatat tgtttgctaa
    69361 aaataaaaaa tagccttaaa acagttgatt ataaaaaatt aactgcttta aggctatttt
    69421 tatctttgtg acgaaataga cacttgccat ttagaatgtg acaatatagt agttgtttag
    69481 aaaaggcaca aagcattata ctttaactaa agtaaaatgt attttgcttt tagaataaaa
    69541 acaattagga gggagctata attgaaaaag ataatatatg tattatttac aattgaacta
    69601 ttaatagtat ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta
    69661 tataacgaga caacaagtat atctgtaatg tttaaagatt ataaaaagtt aaataaaaat
    69721 tatagtaagt ggattaaaga tattgcagat gaaaataatg tgactatatc aaagtatgtt
    69781 tttaataata atgaaaaatt atctatatat acgactgata caagtttgaa taacaatata
    69841 aagttaaaat caggaaaatt tcctaatact aattcaaatg aatttattag taatataaac
    69901 actaataata gtaatcaagt aggtaaattc tcaactatga gtaaagacat atcaatttta
    69961 attagggatt ttggtaaaat aagtgaagtt ggagaatcag gaatcttata tatttcaact
    70021 caaaatgaag atacaataaa taaaatatta agagaactaa atgtagacag aagtattttt
    70081 gtagcaagat tacatgatag atatgttaac tcaaatttat acttaaatcc ttcaataatt
    70141 agagatttaa tactaattat cctttgtttc ttggctacaa taatacatta ttcaattagt
    70201 gtgagtagag aggcttctat tttaagatta aatggatact ctaagctaga tataattgct
    70261 agagttataa aaaatatact tagaattatg attttatcat ctgtcacagc attattaata
    70321 tatataatct atattttaaa attaaatata ggtgttagag catgcttata ctttgctatg
    70381 ttttcgatta tatgtatttt gataaatatt ttgatatcta tattaatagt tggttttaat
    70441 agtagaagtt ctaaatatgt gctatctcta aatggaaaaa aatcttatgg ttttgtaaat
    70501 ataatgcatt tcacattgaa aattgtattt gtatcatttc ttatattatc cataaataac
    70561 tgtattttaa attataatat gctacaaact cagttaggaa atctatctga gtgggataaa
    70621 gcaaaaaatg tttataatct tactcttaaa gatacaggcg aacaatcttt aggaaaggaa
    70681 gaagtaatta aaaatgccaa gattaaaggt ttctataaaa atttggtaga agaaaaagat
    70741 gctttcttaa tagattcaac taattttgaa aaacttgaag atggaagtta catgtatgaa
    70801 ataaattcaa agggtaaaaa cccagaagta agtcaatatg gtaaaaatat aaaaataaat
    70861 aaaaactatc ttaaagttaa ccctatttac tcaaatggca aagaaatatt taatctaatc
    70921 gattatgacg ataagactat aaatttatta gttcctaaaa agttacaagt acatgaaaat
    70981 gagattaaaa aagagtttag agaattattt tattttgaaa aaatagaagt agaaaatatg
    71041 tacaataaag agttaaatag agcgttaaac aaaacaaaga agtcagattt aaatgttaat
    71101 ataatatatg tagataataa tcaaagctat tttacttata actcccttgt tatggatgac
    71161 aataggaatc tcattgatga cccaatagca atagttgaca tagataatat tgatgattct
    71221 ttttatcttt cttatatatc tagatgtgta tattttaatt ctaagaaatt agatgccctt
    71281 gcagatataa gtagcattat tgaagctcaa ggtgtagaag cgcatatcca aagtttacat
    71341 gctgtttata atgagtatgg tttagaaata aataaactag aaaaatattt gaatagtgaa
    71401 atttttacta taataataat agcaatatca aacttaatga ttacctacaa tatagtagct
    71461 agctactatg aaagaaataa atataagtta tatattaaaa aaatatttgg ttattcaact
    71521 acagctagaa gtatgttgtt aatagtctca ttaatattaa caaacattat tccaataggc
    71581 attatatcaa caagagtaga tttaagtaat aatattatct tatttggact tctaatatta
    71641 gttatagaag taattgtatc tattgtatta gacaagatta tctcaaatag ttcatttaat
    71701 aaaattataa aaggagaaca ttaaaaagat gatttcatta ataaatatat ctaaaaaatt
    71761 taaagataaa tctataatag acaaatttaa cctagaaatt tctaagggcg agtttgtagc
    71821 gataacagga aatagtggtt caggtaagac aactttactt aatataatgg gattattaga
    71881 aaaacctaat tctggtgata ttataataaa taatataaaa aatccaaata gtaaacagat
    71941 aagaatttta caaagggatt tttatggcta tctatttcaa aattatgctt taattgagaa
    72001 tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat caaaaaaata tcaaccataa
    72061 taaaaaaata aattacgcac ttgagtctgt aggtcttaga aaatacaata aaaaaaagat
    72121 atacgaattg agtggaggag aacaacaaag ggtagcatta gcaagagtaa tattaaaaga
    72181 atgtgaagta atatttgcag atgaacctac aggtaattta gataaaaaaa atagagacat
    72241 agtatttgat atacttaaaa gtctaaataa gaatggtaaa actatcgtat ttgtaactca
    72301 tgatttagag ttatcggagc aggcagatag agttataaaa ttgtaataat atcaactata
    72361 ataaatataa gaaagagatt acagctaaat caagtttaat aataatacga atagaattta
    72421 aacattaagt aaattagtag tttgtaaata taagtataat atgatatcat aaaggctcaa
    72481 aagaaatttt atataataag aaatctaaaa ataacaaatg gaggacatat aaagatggaa
    72541 aatttaccaa attgcccgaa atgtaattca gaatatactt atgaagatgg aactctttta
    72601 gtttgtccag aatgctctca tgagtgggca ttaggattgg gagctgaaga agattcaaat
    72661 attatcaaag atgtaaatgg aaatgtatta aatgatggag attctgttac agtaattaga
    72721 gaccttaaag taaaaggaag ttcatcatct ataaagattg gaacaaaagt gaaaaatatc
    72781 cgtttaatcc atgactcatc agatggacat gatatagaat gtaagataga tggttttgga
    72841 gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat attataaata agctgtagtg
    72901 tttattgttt ttcagtacag tataaaacca taagaatcta ttaagtaata aaagtttgtt
    72961 tatttatctt tatattttct tatggtttta ttaaatataa attttattga acaccaaaat
    73021 attctctcct ctacaaataa gccaatctta tttaaattaa tatactttgt actatttata
    73081 ctgaaattgt aaatttaaat gctatgttat ttaggtttgt atagttaatt tatatatttc
    73141 t
//
LOCUS       DAMKMI010000004        64034 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMKMI010000004 DAMKMI010000000
VERSION     DAMKMI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 64034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.004"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            215..1090
                     /locus_tag="PC429_000262"
     CDS             215..1090
                     /locus_tag="PC429_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDF3845787.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1108..2598
                     /gene="cls"
                     /locus_tag="PC429_000263"
     CDS             1108..2598
                     /gene="cls"
                     /locus_tag="PC429_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF3845788.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2791..3075
                     /locus_tag="PC429_000264"
     CDS             2791..3075
                     /locus_tag="PC429_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF3845789.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3108..4736
                     /gene="groL"
                     /locus_tag="PC429_000265"
     CDS             3108..4736
                     /gene="groL"
                     /locus_tag="PC429_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF3845790.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            4998..5738
                     /gene="larB"
                     /locus_tag="PC429_000266"
     CDS             4998..5738
                     /gene="larB"
                     /locus_tag="PC429_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF3845791.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5822..6556
                     /gene="larC"
                     /locus_tag="PC429_000267"
     CDS             5822..6556
                     /gene="larC"
                     /locus_tag="PC429_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF3845792.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6586..7005
                     /locus_tag="PC429_000268"
     CDS             6586..7005
                     /locus_tag="PC429_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF3845793.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      7065..7166
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7387..8922
                     /gene="guaA"
                     /locus_tag="PC429_000269"
     CDS             7387..8922
                     /gene="guaA"
                     /locus_tag="PC429_000269"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF3845794.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            9461..10891
                     /locus_tag="PC429_000270"
     CDS             9461..10891
                     /locus_tag="PC429_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3845795.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(11039..11389)
                     /locus_tag="PC429_000271"
                     /pseudo
     CDS             complement(11039..11389)
                     /locus_tag="PC429_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            11384..11905
                     /locus_tag="PC429_000272"
     CDS             11384..11905
                     /locus_tag="PC429_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF3845796.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(12034..12828)
                     /locus_tag="PC429_000273"
     CDS             complement(12034..12828)
                     /locus_tag="PC429_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3845797.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(13052..16480)
                     /gene="uvrA"
                     /locus_tag="PC429_000274"
     CDS             complement(13052..16480)
                     /gene="uvrA"
                     /locus_tag="PC429_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3845798.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHQDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLKGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            16799..19033
                     /locus_tag="PC429_000275"
     CDS             16799..19033
                     /locus_tag="PC429_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3845799.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            19289..21319
                     /locus_tag="PC429_000276"
     CDS             19289..21319
                     /locus_tag="PC429_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3845800.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            21316..21768
                     /locus_tag="PC429_000277"
     CDS             21316..21768
                     /locus_tag="PC429_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3845801.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            21770..22825
                     /locus_tag="PC429_000278"
     CDS             21770..22825
                     /locus_tag="PC429_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF3845802.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            22892..23200
                     /locus_tag="PC429_000279"
     CDS             22892..23200
                     /locus_tag="PC429_000279"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF3845803.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            23304..24569
                     /locus_tag="PC429_000280"
     CDS             23304..24569
                     /locus_tag="PC429_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF3845804.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            24657..25373
                     /locus_tag="PC429_000281"
     CDS             24657..25373
                     /locus_tag="PC429_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF3845805.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(25568..25861)
                     /locus_tag="PC429_000282"
     CDS             complement(25568..25861)
                     /locus_tag="PC429_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF3845806.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(25867..26082)
                     /locus_tag="PC429_000283"
     CDS             complement(25867..26082)
                     /locus_tag="PC429_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845807.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            26531..26929
                     /gene="tnpA"
                     /locus_tag="PC429_000284"
     CDS             26531..26929
                     /gene="tnpA"
                     /locus_tag="PC429_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF3845808.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            26926..28089
                     /locus_tag="PC429_000285"
     CDS             26926..28089
                     /locus_tag="PC429_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3845809.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(28473..28985)
                     /locus_tag="PC429_000286"
     CDS             complement(28473..28985)
                     /locus_tag="PC429_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3845810.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            29435..29911
                     /gene="purE"
                     /locus_tag="PC429_000287"
     CDS             29435..29911
                     /gene="purE"
                     /locus_tag="PC429_000287"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF3845811.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            29917..30615
                     /locus_tag="PC429_000288"
     CDS             29917..30615
                     /locus_tag="PC429_000288"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF3845812.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            30627..31994
                     /gene="purF"
                     /locus_tag="PC429_000289"
     CDS             30627..31994
                     /gene="purF"
                     /locus_tag="PC429_000289"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF3845813.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            31988..33064
                     /locus_tag="PC429_000290"
     CDS             31988..33064
                     /locus_tag="PC429_000290"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF3845814.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            33058..33651
                     /locus_tag="PC429_000291"
     CDS             33058..33651
                     /locus_tag="PC429_000291"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF3845815.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            33644..35176
                     /gene="purH"
                     /locus_tag="PC429_000292"
     CDS             33644..35176
                     /gene="purH"
                     /locus_tag="PC429_000292"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF3845816.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            35187..36437
                     /gene="purD"
                     /locus_tag="PC429_000293"
     CDS             35187..36437
                     /gene="purD"
                     /locus_tag="PC429_000293"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF3845817.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTRVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            36462..40268
                     /locus_tag="PC429_000294"
     CDS             36462..40268
                     /locus_tag="PC429_000294"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF3845818.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDNKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVIGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            40474..41346
                     /gene="rfbD"
                     /locus_tag="PC429_000295"
     CDS             40474..41346
                     /gene="rfbD"
                     /locus_tag="PC429_000295"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF3845819.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            41455..42324
                     /gene="rfbA"
                     /locus_tag="PC429_000296"
     CDS             41455..42324
                     /gene="rfbA"
                     /locus_tag="PC429_000296"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF3845820.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            42373..42930
                     /gene="rfbC"
                     /locus_tag="PC429_000297"
     CDS             42373..42930
                     /gene="rfbC"
                     /locus_tag="PC429_000297"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF3845821.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            42968..43951
                     /gene="rfbB"
                     /locus_tag="PC429_000298"
     CDS             42968..43951
                     /gene="rfbB"
                     /locus_tag="PC429_000298"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF3845822.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            43981..44649
                     /locus_tag="PC429_000299"
     CDS             43981..44649
                     /locus_tag="PC429_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF3845823.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            44658..45086
                     /locus_tag="PC429_000300"
     CDS             44658..45086
                     /locus_tag="PC429_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF3845824.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            45110..45424
                     /locus_tag="PC429_000301"
     CDS             45110..45424
                     /locus_tag="PC429_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF3845825.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            45576..45851
                     /gene="flgM"
                     /locus_tag="PC429_000302"
     CDS             45576..45851
                     /gene="flgM"
                     /locus_tag="PC429_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF3845826.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            45856..46272
                     /locus_tag="PC429_000303"
     CDS             45856..46272
                     /locus_tag="PC429_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF3845827.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            46290..47600
                     /gene="flgK"
                     /locus_tag="PC429_000304"
     CDS             46290..47600
                     /gene="flgK"
                     /locus_tag="PC429_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF3845828.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            47619..48551
                     /gene="flgL"
                     /locus_tag="PC429_000305"
     CDS             47619..48551
                     /gene="flgL"
                     /locus_tag="PC429_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF3845829.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            48567..48959
                     /locus_tag="PC429_000306"
     CDS             48567..48959
                     /locus_tag="PC429_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF3845830.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            48953..49165
                     /gene="csrA"
                     /locus_tag="PC429_000307"
     CDS             48953..49165
                     /gene="csrA"
                     /locus_tag="PC429_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF3845831.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            49187..49582
                     /gene="fliS"
                     /locus_tag="PC429_000308"
     CDS             49187..49582
                     /gene="fliS"
                     /locus_tag="PC429_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF3845832.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            49582..49938
                     /gene="fliS"
                     /locus_tag="PC429_000309"
     CDS             49582..49938
                     /gene="fliS"
                     /locus_tag="PC429_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF3845833.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            49959..51482
                     /gene="fliD"
                     /locus_tag="PC429_000310"
     CDS             49959..51482
                     /gene="fliD"
                     /locus_tag="PC429_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF3845834.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            51485..51820
                     /gene="fliT"
                     /locus_tag="PC429_000311"
     CDS             51485..51820
                     /gene="fliT"
                     /locus_tag="PC429_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF3845835.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            51922..52794
                     /locus_tag="PC429_000312"
     CDS             51922..52794
                     /locus_tag="PC429_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF3845836.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            52887..55028
                     /locus_tag="PC429_000313"
     CDS             52887..55028
                     /locus_tag="PC429_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3845837.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            55052..56929
                     /locus_tag="PC429_000314"
     CDS             55052..56929
                     /locus_tag="PC429_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3845838.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            56922..59036
                     /locus_tag="PC429_000315"
     CDS             56922..59036
                     /locus_tag="PC429_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF3845839.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            59045..60040
                     /locus_tag="PC429_000316"
     CDS             59045..60040
                     /locus_tag="PC429_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF3845840.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            60132..61364
                     /locus_tag="PC429_000317"
     CDS             60132..61364
                     /locus_tag="PC429_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF3845841.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            61384..62328
                     /locus_tag="PC429_000318"
     CDS             61384..62328
                     /locus_tag="PC429_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF3845842.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            62422..62829
                     /locus_tag="PC429_000319"
     CDS             62422..62829
                     /locus_tag="PC429_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF3845843.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            62916..64007
                     /locus_tag="PC429_000320"
     CDS             62916..64007
                     /locus_tag="PC429_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF3845844.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
BASE COUNT        25412 a         6735 c        11447 g        20440 t
ORIGIN      
        1 gaatttcgat aagtttaagt gggtataaac ttatagaaat tcatttttta tttaaggtaa
       61 aaatttaaag taaaatatta ttttacataa taataacctt ataaattttg aaattactta
      121 tctttgtgtt aaaataagta aaatgcacat attagagcta agtttttaag taaataaaca
      181 aaatgaaaat ataaatatta aggagatttg gtatatgaat gtttatgaaa aaggtaatgg
      241 agttatacta gaaggagtaa cagattttga cccagtacat atatttgagt gtggtcaatg
      301 ttttagatgg cacaaacaag aagatggctc atacactgga gttgcaaagg ggagaatatt
      361 aaatgtcaaa aaagagaatg ataaaatata tttaaataat actaatttaa aggagtttaa
      421 cagcatttgg tataattact ttgatttagg aactgattat acagaaataa agaataagct
      481 aaaaaatatg gatgaatatt taaataaggc tactgaattt ggatggggaa taagaatatt
      541 aagacaagat ggatgggaaa tgcttatatc atttataatt tcctctaata ataggattcc
      601 tatgatacaa agagctatag aaaatttatc aagaaaattt ggtaagtata taggtgaata
      661 tgaaggtaat gaatactatg cattcccaac accagaagaa ctaaataagg cctcacaaga
      721 ggaaataaga gcatgtcaaa caggttttag agataagtac ataaaaagta ctacacaggc
      781 agttatagag aataatgatg aggtatctga atatacaaat ttaagtactg aggattgtag
      841 aaaagagctt ttaaaattta atggagtagg accaaaagta tgtgattgta ttgcattatt
      901 tggtatgcaa aaatatgatt cttttccagt tgatgtttgg gttaagagag ttatgcaaga
      961 attttatata gatgaagaca tgagtttacc taaaatgaga acttatggaa tcgataaatt
     1021 taaagaaatg tctggatttg ctcaacagta cttattttac tatgcaagag aactcggtat
     1081 aggaaaataa attaaggggg agaaattatg ggagtaatag gaacaatatt tctgttctat
     1141 ttaattatat catatctagc tggagccata atttctgtga ttatattgct tgaaaataga
     1201 gaccctgcaa aaacaatgtc ttggctattg atgtttatta tatttccagg tgtaggttta
     1261 atgatatatg ctatttcagg tagaaatatt agaaaaagaa aattatttaa gactcaaaaa
     1321 ctagctaata atataaaaga aaagaaacta tttgatacat tagaaaagat aacagaaata
     1381 gttgaattag aaaaagaatc tataaaacaa aataagcttt taagagatga agaggatggt
     1441 agttatagaa aaagggtaat aaatatgttg ttaaagactg gtatgtttcc ctttactaaa
     1501 aataataaag tggatgtatt tgtagatgga aatgaaaagt ttaaaagact tatagaagat
     1561 ataagagaag ccaaagacca tattcattta gagtacttta tcatcaaaga tagtgaaata
     1621 ggtagggtgt taaaagaaga acttataaaa aaggctaaag aaggcattaa gataagaatt
     1681 ttatatgatg atgtaggatg ctggagattt tggtttaata gaaaattttt ccgagaaatg
     1741 agggaagtag gtatagaaat tgcagcattt ttacctacaa aatttcctat aataggaggg
     1801 aaattaaact atagaaatca cagaaaaatt gttgttattg atggaataat tggatataca
     1861 ggtggaataa atataggaga tgaatattta ggcaaaaatg ataagtttgg atattggaga
     1921 gatactcata ttaggattaa gggtatttct gtatacatgc ttcaaatgac atttctgatt
     1981 gattggtatt atacgacaaa agaagtatta gtgactaaaa attatttccc aagtgtaggg
     2041 aatgttggtg aaagtatgat acaagttgtt gcgagtggtc cagacagtga ctgggaagat
     2101 attcattatg cttatttttc tgcaatatgt caggctagaa aaaatgttta tatagaaact
     2161 ccatatttta ttccagatga aagtctacta aaagctataa agagtgctgc tcttagtgga
     2221 gttgatgtga gaataatatt tcctaaaatt gcagaccaca agatagtaaa tatagcatct
     2281 tattcatatt ttgaagaaat attaagagca ggtggtaaag tttatttata taataaagga
     2341 tttatacatt ctaaagtcgt aataatagat gataaaattg cttctgcagg tacagctaat
     2401 atggatttaa gaagttttat gcttaacttt gaggtaaatg catttatcta tgatgaagaa
     2461 gttattaggg taatgacaga tgatttcttt gaggatttaa gtcactgtga agaacttaat
     2521 ttggaggtat ttaagaatag aaatatcata caaaaaatca aagaatctgt agcaagatta
     2581 ttttcaccaa tattataaaa aattattaaa aaaggtattg aaaattatgt gtgattggta
     2641 tattataata attgttagca ctctattaga tagagtgata ataaataaaa agtatatata
     2701 taaatctaca tatataagta gtattaattt aaaaataact aaaaacatag gcgaactagt
     2761 ctatacttaa tattttagga ggcgtgaata atgaaaataa gaccattagc tgacagagta
     2821 gtaattaaaa aagtagaagc agaagaaaaa actgcaagtg gaatagtttt accaggagca
     2881 gctaaagagc aacctcaaat agctgaagtt gtagaagttg gaccaggtgg aatagttgaa
     2941 gggaaagaaa taaaaatgga attgacagtg ggagataagg ttatattcca aaaatattct
     3001 ggaacagaag ttaagataga aggacaagaa tacacaatac taagacagag tgatgtatta
     3061 gctgttattg aataaatata gaataaattt attaggaggg gtttaaaatg gctaaagaaa
     3121 ttaaattttc agaggaaaca agaagagctt tagaagctgg tgtaaataaa ttagcagata
     3181 cagtaaaagt aacattagga cctaaaggaa gaaatgttat attagataaa aaatttggtt
     3241 ctccacttat aactaatgat ggagtaacta tagcaaaaga gatagaacta gaagatagat
     3301 ttgaaaatat gggtgctcaa ttagttaaag aagtagcaac taaaactaat gatgtagctg
     3361 gagatggtac tacaactgct acagttttag cacaagctat aataagagaa ggtttaaaga
     3421 atgtaacagc aggggctaac ccaatacttt taagaaaagg aatacaaaaa gcagtaacag
     3481 tagcagtaga agaattaaaa aatcaatcaa gaatagtaga aacacaagag gctatatctc
     3541 aagttgcttc tatatctgct ggtgatgaag aagttggaaa attaatagca gaagctatgg
     3601 aaatagtagg taaagatgga gttataactg ttgaagagtc tcaaactatg aatactgaat
     3661 tagatgctgt tgaaggtatg cagtttgata gaggatttgt ttctgcatat atggttacag
     3721 atgtagataa aatggaagca gttttaaatg acccatatat attaattact gataaaaaaa
     3781 tatctaacat acaagaatta ttaccagttc ttgaacaaat agttcaacaa ggtaaaaagt
     3841 tattgataat agctgaagat gtagaaggtg aagcattatc tacattagta gtaaataaat
     3901 taagaggaac atttgatgta gtagcagtta aagctccagg ctttggagat agaagaaaag
     3961 aaatgcttca agatatagca atacttacag gtgctcaagt aatatcagaa gaattaggtt
     4021 atgatttaaa agaagctgat ttatctatgc taggtagagc ttcatctgtt aaggtaacta
     4081 aagaaagtac tacaatagta gatggttctg gagataaaaa agctatagaa gatagagtaa
     4141 ctcaaattaa acatcaagta gagcaaacta cttcagattt tgatagagag aaattaatgg
     4201 aaagattagc taaacttgct ggaggagtag ctgttgtaaa agttggagct gctacagaag
     4261 tagagttaaa agaaagaaag ttaagaatag aagatgctct taatgcaact agagcagctg
     4321 tagaagaagg aatagtagct ggtggaggaa ctgcttttgt tagtgttata ccagcaatag
     4381 gaacactaat tgagagttta gaaggagaag ttaaactagg tgctcaaata gttaaaaaag
     4441 cgttagaaga gccattaaga caaatagcta taaatgctgg tcttgaaggt gctgtaattg
     4501 tgcaaaatgt tgttaattct gaagcagaaa ctggatttga cgcattgaat gaaaaatatg
     4561 taaatatgat agaagctggt atagttgacc caactaaagt tagtagaagt gctttacaaa
     4621 atgctgcatc aatagcaagt actttcttaa ctactgaagc tgctgttgct gaccttcctg
     4681 aaaaagaaga tgtaggaatg ccaggaatgg gtggcggaat gcctggtatg atgtaattta
     4741 aaaaatttaa aatataaatt ttaatataaa gaactatttc gacttagatt ttaaactcta
     4801 agttgaaata gttcttttgt atatatctaa tttttgtata tctataaatt caatacacat
     4861 atttagataa tcagtatcta gtttgtaata atattttaga gtagtattgt tattttttag
     4921 gttatgattt tagatatata ttaattaagg tataattgtt gatagagaat aaatcaaatt
     4981 taaaaggatg cgataatatg gacttaagaa aattgcttga agaagttaaa aatgaaaata
     5041 ttgatataga ttttgcgtta gaaaagttaa aagatttacc ttatgaagac cttggatatg
     5101 caaatataga ccatcataga gaattaagaa atgggtatcc ggaagttatt tattgtgaag
     5161 gtaagagtga tgagcatatt ttagggatta ttgataaaat gaatcaaaaa agttctaaca
     5221 tattggggac aaggtgcaga aaagaaactt ttctgaaaat taaagaaatc tataatcatg
     5281 ctgagtacga agaggcgtct aaaattctta agattcaaaa tcatgatata gagaatatag
     5341 gaaaaggtaa aattgtgatt gctacagggg gaacttctga tataccagta gcagacgaag
     5401 cttatcatac agctaagttt ttaggtaatg atgttgatag aatatatgat gttggagtgg
     5461 caggtataca taggctttta aataaaagac ataaaataga tagtgctaga gtaatagtag
     5521 ccgttgcagg tatggaaggt gctcttgcaa gtgtaattgg tggactggtt gatgttccag
     5581 taatagctgt tccaacttct gttggatatg gagcaaattt tggaggatta gcagcattac
     5641 tagctatgtt aaatagctgt gcatctggaa tatctgttgt aaatattgat aatggatttg
     5701 gagcaggata tttagcatca atgataaata aactgtaaat acattgatat aaattaaaca
     5761 aaactatata ataaaattta atttctatta tatttttact attaaaattg aggagacata
     5821 tatgagtgaa aaaatacttt attttgatat aataagcggt atatcagggg atatgaccct
     5881 tgcaagttta ttaaatttag gtgtgcctaa ggaaattttt ttggaggaaa ttcataaact
     5941 aagaatgagc gatgaattta atataaacat cagctcgaaa actgaaaatg gaatagtagg
     6001 tacaaaagta gaagtaatta caaaagaaaa acatacacat agaaatttag tggatatatt
     6061 tgaggttata gataaaagca ctctaaacga aaatgtaaaa tctaaagcaa aaaaaatatt
     6121 tatggtaatt ggagaagcag aagcaaaagt tcatggaact acaatagaca agatacattt
     6181 tcatgaggta ggggctatag attcaattgt agatatagta ggagcttcta tattagttga
     6241 cttactttgt gtagataaag tttgtgctag tacagtgtct gttggttcag gatttgtaaa
     6301 atgtgaacat ggaattatac ctatacctgc acctgcaact atagaaattt taaaaggtgt
     6361 gccaattaaa ttaaataatg taaatggaga atgtacaact ccaacaggag ctgcaatcat
     6421 aaaagtcttg tgtgatgagt ttgtagatga gcttgaattt tctccaaagc aaattggata
     6481 tggtataggt cataagaagt ttgaagtacc aaatatactc agaactgtat taggtgaaaa
     6541 aaaaaagaag aattaattta tgaaattagt gcaaacattg atgatatgtc ttcagaaatc
     6601 tattcttatt tgtatgaaaa aatattagat gagaaagcat tagatattta tacagagagc
     6661 atttatatga agaagaatag accagcaaca aaagtatcaa ttttatgtaa tgaatcagat
     6721 ttagaaaaat ttattaaaat attactttta gaaactagta cttttggagt gagatataat
     6781 gcatataata gagaaattct agagagaaaa tttgtaaaat tagatacaaa atatggagtg
     6841 gttacagtaa aactagggta ttataaagga aaactagtca aggctactcc agaatataat
     6901 gaatgtaaaa ctatagcaaa aaatgaagat attcctataa aaaaagtgtt tgctgatata
     6961 aattttagaa taaaagaaga atttagtgaa aacttattga cataagtaat aaattttgat
     7021 aaattaatat tcagccttat taaataaaag taaacagaaa aaaatatata aaatataaac
     7081 tcactcgtat atgctcagaa atatggtctg agagtctcta ccaagatacc gtaaatatct
     7141 tgactatgag tgaaattatt ataccaaaag acctagttaa gtaaatgtgt aggctgagtt
     7201 aaactcccta tgtgttggct ataagataaa ggtattttgg aagcctgtat agtaatttca
     7261 cttttgctag aaattatgga agttactata caggtttttt gtgtaaaaag atttaatata
     7321 atttagactg tattgtgtta aaataaatat ataaaaacta tattatacaa ggagagatgt
     7381 aaatttatga aacatgaatt agtacttgta atagattttg gaggacaata taatcagttg
     7441 atagctagaa gagttagaga aaacaacgta tattgtgaaa tacttccatg tactgcaagc
     7501 atagaaagaa taaaagaaaa aaatcctaaa gggataatat ttacaggcgg tccaaatagt
     7561 gcgtatttag aagattctcc aacaatatca aaagaaatat ttgaactagg agtaccaata
     7621 cttggaatat gctatggtat tcaaataatg tctcatgtat tgggtggagt tgtaagaaaa
     7681 ggtaataaac aagaaaaaga atatggaaaa acagctataa cttatggaaa atcatcatta
     7741 tttgaaggaa taactacaaa tagtgtatgg atgagtcata cagatttaat agaaaaagtt
     7801 ccagaaggat ttactatagt tgcaaatact aatgattgtc cagtagcagc tatggaaaat
     7861 gtagaacgaa acctatatgg agttcaattc catccagagg tagaacactg tttggaaggg
     7921 gacaagatac ttacaaactt cttatataat atttgtaaag taaaaggtga ctggactaca
     7981 gattcattca tagaagataa aataaaagaa ttaaaagaaa aaataggcga taaaaaagct
     8041 ttatgtgctt taagtggagg agttgattct tctgttgctg cagttcttat tcataaagca
     8101 attggggaca acttaacttg tatatttgtt gaccatggtt tacttagaaa aaatgaagga
     8161 aatgatgtag aaagaatatt tagagagaaa tttgatataa atttaataag agttaatgca
     8221 gaagatagat tcttatctaa attaaaagga gtttctgaac cagaagctaa gagaaaaata
     8281 ataggtgaag aatttataag agtatttgaa gaagaatcaa ataaattagg aaaaatggat
     8341 ttcttagtac aaggaacaat ctatccagat gttatagaaa gtggacatgg aaatgctgct
     8401 actataaagt ctcatcataa tgttggtgga atcccagaag atgtagactt ccaagaaatt
     8461 gtagaaccat taagagagtt atttaaggat gaagttagaa aaataggatt agagcttggt
     8521 atagaagaag gattaatatt tagacatcct ttcccaggac caggtctagg aataagagtt
     8581 ataggtgatg taactaaaga aaaatgtgat atattaagag aagctgatgc tgtttatatg
     8641 gacgagctta gaaaagcagg tttatacaga gaaatatggc aagcttttgc aacattacca
     8701 gatgttaaga ctgttggagt tatgggtgat gaaagaacat atgcttattt agttggttta
     8761 agagcagtta cttcttctga tggaatgact tctgactggt ataagatgcc ttatgatgta
     8821 ttagaaagaa tttctaatag aataataaat gaagttgatg gagtaaatag agtagtttat
     8881 gatataactt ctaaacctcc tggaactatt gaatgggaat aaaatataga atgccaaaaa
     8941 tccacataaa tacaggattt gaggtctctc ttacttctat gttatactgg tttgatacta
     9001 tcaatatttg ccagtatctt atggattaat aagcaaaaaa catcctgctg aatgataaat
     9061 tcagcggggt gtttttattt tgttttagaa ctcctttgct ctgaaactcg aagtatttaa
     9121 cactgatgat tttgttatct tttagttcct tttgtgtcat gctctgaaag acaaattgat
     9181 acaataaaaa agtaatttct taatcattga tggagatatg tgtttatgtt actcttgatg
     9241 gtaatttgag taatattgtt catagctttt tatattcact taattagaaa accatttggt
     9301 ataatcataa ataaacttga tgatataata gaaaagattt ataattatta gaagtatata
     9361 aaagtatttt atgtctatga aatgtatgtc tataaaacat atagatgact tttgtatatt
     9421 aatgtatgaa tattgatatt atagaagaat tgagttgtca atgatactaa atcaaatatt
     9481 tatagaagct ggagggatta tgagaaataa aataattata ggaattactt tttcacttgt
     9541 attactaatg tctggatgtg cttcaaaaca aaccgcagaa ataaacgtat tagcaactac
     9601 tgatttacat ggagttatac cttatgagtt aacttcttat gtaaaagaag aaagaaaaaa
     9661 agataaaaat ctaactttag tagatgcagg agattttttt gatagtggag aagtgtttgg
     9721 ttctagcatg gatagttatt ttagtgctag atataaagat ggtgataata acactgaaag
     9781 ttatattgag acgcctattt caaaagatat gaaagaggta ggttatgatg ttgcagttct
     9841 tgggaatcat gaatttatat ccaacaataa attttatcta gataatatga tatctgattt
     9901 tgagaagcaa aaaataaata ttttatcggc aaatacgtat aaaaaaaatg gagatagtta
     9961 tacaaaacca tacataataa aaaatattaa tacacctgaa ggaaacgtaa aactaggaat
    10021 tttagggctt actataaaag aagttggtga gaggaaacag tggaaagatg gaaaacttgt
    10081 tgactctaag tctttagaac taaaagacca agagggttac aatggtgaat tgtatatgaa
    10141 tgatttagta gaagatgcta aaaagtgggt tggtataatg gaaaaagaga agcctgacat
    10201 tatagttgct gtggcacata caggtgagaa acctaagaaa cctaaaaacc caggaaatag
    10261 aatacaagac ttagctcaac aagttgatgg aattgatgct atagttgcag gtcataatca
    10321 tgttcaaatt gagcaacatg attataaaaa taaatcagga gaaaatgtta tagttactga
    10381 gccaggaaag catggggaat gtatttctaa gattaatttt aaattagaaa aaaacaatga
    10441 taaatggaat atagttgaca aatctagtaa attaattaaa tttgaaaaaa atcaagaaag
    10501 tgataaattt ggagaattta tttcttcaat taataaagta agtaaagata agaaagaaat
    10561 aagattatca gaagtaaaga ctcctgaatg ggataaaata tatgttttta aatctggaac
    10621 atctgcagat gatatatata aaacaatagg atataaatgg agaaatatat catctccaaa
    10681 gggtgatgac atggtgcaga ttgttgtaat gaataataat aagcctgtta gctatgtata
    10741 tggtgaagct gaagttatgc caattaatat aaaatgtgat gaatcagagt ataaagataa
    10801 tgttgtaact atttatccta ataaaaatga taaatttaaa attgaaaaag gtaaacaaga
    10861 atatagtatt aatttaaaac atatacaata gaaactttta tcttatttaa gtttagattg
    10921 tacttaaaat aaagtgtgta ttattttaaa aattgatgat aattaaaatc acaacattaa
    10981 agaatagtac acacttttta tgctaaaaaa tagaatgtta aaaaattaaa cctttaaatt
    11041 aaagtacatt gcttatatta tccaatatta ttttcaaagg ttgtgtaata tttttatcct
    11101 tatgtaatat tgctaacatt ttattttcca tatctatccc atctaattca atcaatcgca
    11161 attttttatg taagagtgaa tctaataata aattttctgg caaaatggtt atacctaacc
    11221 ctgcttgtgc agctttaata agtgcaaaag aattaacact ttcccaaaca ggataagctt
    11281 tttggtttga aagagataat acactgtcca aagtattgcg aatggcactt ccttgctcac
    11341 gtaacaataa cggaaacgtg cataactgtc gtagagataa ggcttgttta gtattggtgg
    11401 tggttatgca atcatacttt taatccaaga gaaagtagta aatcaaactg gatggataag
    11461 tgagaaaatg tttacagata ttataactat ttcacaaatg acaccagggc cactggctgt
    11521 taatacttct acttttgttg gacttcaaat tggagggttt tggggagcag ttgcagccac
    11581 tttaggttgt gtattatgtg gtgtagtaat atctctattt ttatataatt tttttcaaaa
    11641 gcatcagaac tctacttata tatttgaaat attaaatggt ctaaaatcag cttcattagg
    11701 tttaattatc tcagctgcgg ctacaattat aatccttgca ttatttggga cgaataaagt
    11761 gtacttcaat tctatatttg agatgctaaa ctggactgca ctaataatct tttttgttac
    11821 actactgata gtacgtaagt ggaaaattaa tcctattgtg attataataa taagtggtat
    11881 actgggaata tttttttatt tataagtttt tggtaagtag aggatatcta tttttttata
    11941 tcttaaatcc aaatagccaa taaagtaagg ccatttggat ttttaataaa ttctgaaaag
    12001 ataatcagaa acccatataa catttaaatt atatcactgg ttttctacaa accaatcata
    12061 agcttcttgg caaaatttct cactatctat atagtaacta ttatgaacat tattaatttc
    12121 aacaagtttc tttttacatt ttagtgaatt ataatagcta tcaaaatatt gggttttaaa
    12181 atatctatca tgaagtgttt taaagacaag agtaggtatg ttgactccat cttttatctt
    12241 actatctttt gcaataaagc attctaacat tgtattaatt atctttgtac tatttttcca
    12301 aatatgagga tttttctttt caaatatttt ataaaattca ttatcataga cttcattata
    12361 acctttatat ttaggtggtt ttactgttat gtgaaaatta ggaagaaact taaagaaaaa
    12421 gtttaataat ttaacagtga gataggtata gcctactcct gttgtgtttt tataatcaac
    12481 acaagcaaac tgagcaaaac tttttaactt attgtctgaa catgcataat attgtgcaaa
    12541 cataccacca attcccgtat tcccatatag atgtatgttg gaagaataat ttttttctat
    12601 gtatgtaatg acagaatcta agtcttttac aattgaattg cgagaaaaat ctttttcata
    12661 tccatcactt ttccctgttc ctgaaaaatc aaaagcaaat atatttacat tatgtttggc
    12721 aagtggttca tatgcttttt gcatctcaag cgtagtgaca ataggagtat gtgtaacaac
    12781 aatattcatg gatgattttc tggtaataaa tgagttacaa aaaatcattg tatctccatt
    12841 tggtattttt atatgtttcc aactcatttg attttccttt ctctattata aaatataata
    12901 atttttatgt tctgttttat atattattca tattttatca cagataatat actaaagtta
    12961 atttttttat tgtacaccta ttataataat tcttctataa tataagacat tctataattt
    13021 tatatttaag ttcatgtgta tttaattgtt tttatataaa atgtccagta atactatttg
    13081 gatttatttt tatttcatca ggagttccag aagcaacaat ttcccctcca tttattccac
    13141 tgtctggacc tatatcgata atccaatcag aagcacgtat aacctctgta ttgtgttcaa
    13201 taattaaaag tgaatgctgt tcatttacaa gttttctaaa agtaattaac aaattttcta
    13261 tatctttaaa atgaagtcca gtagtaggct catccaatat gtacagcatt cgttttttag
    13321 tttttgtccc taattctttt gctaatttta tcctttgaga ttctcctcca cttaatccta
    13381 atgtattttg tccaagcttt agatatccaa gtcccacttc atctaataag gagagttttc
    13441 tatatatatc agagcaatcg ctaaaaaatg tttttgcttc tgctacttcc atgtcaagta
    13501 tatctgcaat tgtctttccc ttgtattgta tagacagtat ttctttttga tatctcttac
    13561 cttgacattc actacaagtt acccattggt ctgccatata ttgcagaata atttttgttt
    13621 tacctaatcc ttgacagttt gggcattgcc cctttttgct attaaagcta aaatagcttt
    13681 catccaataa tttttgtttt gcttgtggag tttcagcaaa tagagaacga atttcatcaa
    13741 aaatattgat atatgtagca ggtgtagaac ggctagattt tccaataggt gtttggtcca
    13801 ttaagataaa atcatcaata gcatcaaatc cagtaaattc agtataattt ttatctggga
    13861 tagatttttg tttcaatttt tcttctagag caggaagtaa actgtgaaat acaagagagc
    13921 tcttaccaga accactaaca cctgtaatag agcacataca gtttaaggga atttctacat
    13981 caatattttt taagttattt gcatggcatc cttttaagct taaccattta cttggtataa
    14041 ttttattttg tgaacttatt ttattgtatg tgcttaaata tttacctgtt atagagtttg
    14101 gattttttat aatttcttgg gttgtacctt ctgcaataat gaacccccct tttgtaccag
    14161 cacttgggcc tacatcaata atataatcag cagacagtat tgtatcttta tcgtgttcca
    14221 caactataac agtattaccc ttatctctta gctcttctat cattttgatg attaaattat
    14281 ggtctcttgg gtgtaaacca atagatggtt catctaaaat gtaggttaag cctactaatt
    14341 cacttcctag ctgacttgat aatcgtactc tctgcagttc tcctccagat aaagttggag
    14401 ctgttctatc tgttgatata tagctaagac ctacctttaa tagataacta actcgaattt
    14461 ttaattctgt taagatatca tttaccaaag agagctcatt tttttgaagt tgatttggta
    14521 attcacacaa ccaattccat aactgttcta tcgtcatttt tgttagttca gggaagcgat
    14581 aacctttgat tgtagtgaat ctagcctcaa tgcacagcaa ttcgccacca catgtattgc
    14641 aaggaatttt gttcatatat tgatgcaagg tattattttc agaactagat tctctaaaaa
    14701 gtctttgaat gtggttgatt gctcctgaag caggtcttct aatctttgat tctcgtccct
    14761 ttgattcaaa gctaaattca tatatcttat cgtctgtacc atataagatt gcatctataa
    14821 aatctcttgg taattcattc caaggtatat ctaggtctat atttttatct tttgctattg
    14881 catataattc acctaccatc caattaccat ttggcttttt ccccttcaat tttccaaaat
    14941 agtaagtagc tccatctaat attgaaatag ttgggtcttt tacaatcaat tgaggattaa
    15001 cttgataggt aaaacctaat ccattacaat cgtgacaagc tccagtatgg gagttagcat
    15061 tgaaggttga agtatttatc ttagaaaaaa tagtttgaca atgtggacaa taattttttg
    15121 tattattctt tttttctaat ggaattttac aatatggaca gtaacttttt ccgattgata
    15181 caaataacat tcttaaataa tcccaaatgc ttgtaagagt accaacagta gagcgaggat
    15241 tttggcttcc ttttttctgt tcgatagcta tagtaggggt taatccctga agtaaatcaa
    15301 attccggttt ttgaagctgt tctgaaagtt ttgctccatt acttagattt tcaaaataac
    15361 gtctttttcc ttcttcataa atcgtatcga aagctaaaga agattttcca ctaccactta
    15421 cacctgtaat aacagttatt tgattttttg gaattgtaac ctcaacatcc tttaaattgt
    15481 tttgccttcc tccttttaaa tagatagagc ttttattatg acaaagtgaa tttttaattg
    15541 atttatttat tgattttgta aatttgaatt tcttttcgag ttcaagagga ttgcctaatt
    15601 cttttatttc cgaaatgcct aatattttta actgagaaaa gattgatttc aaataatctt
    15661 caggcaattt ttctattaac aaattaaggt cttctatttt tatatttccg cttgataata
    15721 tttctgccat ttcatctaat aaattttttt ctgctcttgt taattgattg tattttttgg
    15781 aacttaattc tgtccataaa tcccatggaa gcaattctaa accaatcata ttattaaagt
    15841 catgtagaag attacccttt acagaatgcc aacctttcca atttttatta tatccaaagt
    15901 catcttgatg agcatgtcct tgccgacatc ttatccaggc ttctgaacca gttaaaaaac
    15961 catctgtttt tccgacatca tgagtgtcaa aattaatttg aaaataattt ttttgtatgt
    16021 catctatttg tgcatctatt cttatccatc tctttgttag agtatcatag tattctacaa
    16081 gccagtggtc ttcataagta agctcacttt caaaataatt agcaaaacct gctctcattc
    16141 tagcctcaaa acctcgatat cttaggagcg atacaagaag taaagaataa tctctgcaca
    16201 ttccaacaag cctttgtttt gggtttcttg gaatagttag tgggttagaa tttatttcaa
    16261 atattctttt taacatttga ggaattgtac gaagcaattc ctcatcagat tgttgtttat
    16321 taaaggatat accatataat tttccttggt ctccatgaat tactaggctt tgaaccactt
    16381 ttaataactc aattggagtc tttggtaaat cttgatattt attttcaaat tttttaggat
    16441 ttgtgatgat gctttgattt ttatagttat ttattttcat ttatatttca cctctctaaa
    16501 tggatgatat accctgacat ggtgtcatat gcaagggcaa tttaaatttt ttggccaaaa
    16561 tttaaaaata aataaaaaat gatacagtga taaattgtaa tacaaatata gtttcaaatt
    16621 gcatataata taattattta ataaaaccat tatctatgga atgttaatga aatgttaaga
    16681 tggcattctt gaatattaag aaatagaata ctataatata gtgtgaagga tttttagtaa
    16741 aaaaatttgt aagtgttaat atacattaaa aaatagattt ataatttgga ggaaaaaaat
    16801 gaaattatca aggtataaga aaaatagttt agttctgtct ataattttat gttcacttat
    16861 tatttcaatc cttgtaatta ctgtttttaa taccgtaaaa ctacacaata tatctaataa
    16921 acaatctaaa aactatttaa atgatgtatc tacacagatt gtaatgaatg ttgattctaa
    16981 aatcaaattt attttatccg acttaagaat aatggcagat tttattaagc aatatgaagg
    17041 tgatagtcga tatgagtatt tatctaaaag aaagactagt tataagtatt atgatgttgg
    17101 aataatagat ttagaaggta aagctgagtt tttaagtgga aaaaagtttg acttaaagaa
    17161 tactgtttcc tataaaaaag gaattgaagg taaagaatac tcagaagtaa tagagtcaat
    17221 gggttttgtt ctttatagtg tacctatatt taatgataat catgaagtat ctagtatttt
    17281 aataggtgta tccaataaag aaagtatgaa tgagattctt aatatagata actttaatgg
    17341 aaaaggaact attgaaatta ttaatagtaa gggaaaacct cttttcatag gaaaaaatac
    17401 tgaattaata aaggacctta gccataaata taagagttct ggagatgaac cttgggcaca
    17461 aaagatgctt gaggatttta aaaatgataa atcagggaat attacaataa cctcatcaaa
    17521 aggtgtacaa tgcttattaa cttatcatcc aattacaaga ggtttaaatg attggcattt
    17581 tcttctcata gtacctgaag atgctgtact tggtgaactt aataaattaa actcttttac
    17641 aataacaatg acatttataa ttacatgtat tattggaatt ataacattaa ttttatatat
    17701 tataagaaga aaatatgtaa atcaaataga aaatattgca tatttagata gtataacaaa
    17761 tggtataaat tcaactaagt ttagtatgct agtcaaacct ttaatttcag aatcacctga
    17821 tagtacttat atgatgatag caatgaatat aaaagatttt aagctaataa atgattgttt
    17881 cggtagtgaa aagggaaata tgacactaaa acatctttac aatattttgt ataaaaacat
    17941 tgatgaaaat gaagagtttg tgtgccgtca tgatgcagac cttttctatt tgcttattaa
    18001 gaatagacca gtgttagaag ccctggagtt attatataaa atagaagctg acacaaatta
    18061 ttttaataaa aatagggaaa atccatactt tttaagacta tcagtaggta tttacacaat
    18121 tgaaaatcat aatgaagatt taattacaat acaagaccat gcaaatacag caagaaaaag
    18181 ctttaataaa actcatcaaa gtgatttttc tttttatagt gatatagaaa gaaaacgatt
    18241 aatatctgaa aaggaaattt caaatttaat ggaaaaagcc cttgaaaata aagaattttt
    18301 tatgtgctta caacctaaaa ttgatataaa aagtggaaaa attagtggag cagagtctct
    18361 tgtgcgttgg aaaaattcag aaaagggcat aatttatcca agtgatttta ttcctctttt
    18421 cgaaaaaagt ggatttatat gtaagttaga tttatatata ctagaagaaa catgcaaatt
    18481 gatttctaag tggataaaag aaggtaaaga agtgttgaga atttctgtta atgtatcacg
    18541 tcaacatctg aatgataaat tgtttttaga aaaatataag catatatgca ataaatacaa
    18601 tgtaccaaca tgtctgatag atttagaact tactgaatct attttcttag aaaatccaga
    18661 agctatagat ataatagagg atattcattc taatggattt aaatgttcta tagacgattt
    18721 tggatttggg tactcatcat tagggatatt aaaggatttt aaagttgata taattaagtt
    18781 agaccgttca ttctttgtta gcaaaaataa tatagaccgt ggtaaggtag ttattaagtc
    18841 tattattgaa cttagcaaaa ggctaggtat gaaagtaact gctgaaggaa ttgaagaatt
    18901 agaacaagtt gagttcctaa aaaatgtggg atgtgattat attcaaggtt atgttttttc
    18961 aaaaccatta ttaatttctg actttgaaaa ttttgcatat gaaaataata aaattaaaaa
    19021 tatccattat taaatgtaca agataaaaaa tttaggcaag ttatataatt aaaaatataa
    19081 cttgcctttt ttatttatat atttaataaa aatatatttg cagattttgt aaaaataatt
    19141 ttcaaatgtt gtaagttgtg gctaaagctt tattgtatta ttctgaatat agaaagatag
    19201 ttactgtctt agataaaaac tgaaacatac ataataatat aaaatttgct atattaataa
    19261 ctctaattat tttggaagga gggaaaatat gataagtggg agaatgttac tcatcatgaa
    19321 ttttctaaaa ggaaaaaata agacaagtta caaggatatt agcaaacaac ttagtataga
    19381 agaaagaaaa gttagatatg atataaataa tttaaatatt gttttgcgtt cattaaacaa
    19441 gtcagtaata caaaagatga acaaaggtgt gttaattatt cctgatgact tccaagtagt
    19501 atgtataaat aataacgaat atgtattttc cttaactgag cgagtatcta ttatgaaaat
    19561 tatatcaatg tttaaaatag ataatctaaa cttagaaaaa ttaagtaaag aatttcaggt
    19621 ttctagaagt tcaattaaaa atgaccttaa tgttttgtca agtgaattag aaagaaacca
    19681 aataactatt acttatgata aatcttttaa aataagtgga gaagaagaca ttatatataa
    19741 gcaaagattg aacatattaa aatcgtattc ttatcttttt ggtaaggaaa aatctgattt
    19801 aagtgtattt gagaagtatt tagtgaatgt aattgaaaaa atattcaaag ggatttattt
    19861 gattgatata tataattggt ctatgaattt attattgcaa atgggatgga ctttaaatga
    19921 tgaatctttt acatggtatg tgtcaaacat tttccttttt acatggtata tacattcaga
    19981 tgaaaaacat cctttagaat ctgcttcatt aagtgagccg tgttttgaaa ataagtttga
    20041 ccaagaattt gaaacaatta ttcaaaaaaa gttgactaag gaacaaattt ttctgttaaa
    20101 gagttttgta ttttttacaa ataaatatgc aagtttaaac gaggatatgg atttgattag
    20161 tacagaaatt atagttcaag acctaattta caatatgtca gaaatattag agattagctt
    20221 taaagaagat atgattcttt ataaaggatt actcaatcat attgccccat tgatagagag
    20281 aatcaagcag aatatacaga tttatgagga attacttcat gtaataccag ataaatatca
    20341 atatgtactt gaagctacta aggtttctat tcaaaaaaac agagttttaa gtcaaataaa
    20401 gaatgaaaat gaaacaatac ttttggcaat acatttctta ggaagtatac atagaaatga
    20461 gaggaataac tacaaaaata tattgttagt ttgtggattt ggatatggtg taattgctat
    20521 gttaaaggat agtttagtaa atgattacca gataaatata gtagagagta ttccatcata
    20581 taagttacaa gattataaag actgggaaag tattgacttc attataacaa catcaaagat
    20641 agtaatagac ttgcctaaaa caattattga gataaatcca tttttacaag aagaggatta
    20701 tttgaagttt gaagaaaaag ggataaaaag aaagaatgtc ttaactaatt atatgtcaat
    20761 aaaaaaaagg ttggactttt tagaagaaga tgcccaaaaa agagtattaa gtattatatg
    20821 tcaagaactt ggatacagtg atgagagaat attatttaga caacttaatt taagtgattt
    20881 aatagggatt gataccataa ggataattga tggagatatg aaatgggaag atgctgtaaa
    20941 atttagttca aacatacttg ctaattgtga ttttgtatca gcacaatatg ctgagaatat
    21001 tatagatatt ttaaaaaatg tgggttttta tgctgtaaaa gataacgaat ttgctttact
    21061 acatggcaat gatagttcat tagtcaaagt aagttcgata agtttattaa tatgtaaaga
    21121 agcagttagt tttggagata aaaaggttaa aatcatattt tgcctagcaa gcagagataa
    21181 aaaagagcaa attcctgcaa taattaatct aactaaaatg gtttataaga ctaattttat
    21241 tgctatgtta gaaagtgcaa agacaaaaga agaagcagaa gttttaattc ataaatatga
    21301 gaaggaggtc actgtatgaa taaattagtt aatgtaaaat gttcatttaa aatacatgtt
    21361 aattcaaaag aagaagccat tatatcgtta gttgatgtag tgtcaaaaga aggatattta
    21421 atagataaaa atcaattttt aaaagatgtt ttaaagagag aagaaacatt gtcgacttat
    21481 attggacatg gtataggtct tcctcatagt caaagtgtag gtgttaaaaa ctcttgtatt
    21541 acaataggaa agcttgatac tcctattgaa tggacagaag aaggagaaaa agtggattta
    21601 atatttttga tatcagtaac aaaagacaat gaaaataatc ttcatttaaa gattttatca
    21661 aaattagcac gtttattgat gcatgaaagt tttagaaatc aaattagatg ttcagatgaa
    21721 caaactgttt ataatcttat aaaagaaaaa atagaggagg aagattaaaa tgagaatttt
    21781 aaagtttttg caaaaacatc ttatgacagc aacatcttat atgattccat ttgtggttgc
    21841 aggaggaata ttatttgctt tatcagtaac tttaagtgga caagctgctg ttcctgaaac
    21901 aggctggttg gctaaactga atcaaattgg agctgctggc ttggctttat tcattcctat
    21961 attaggtgga tatatagcat ttagtatggc agataaacca ggattagctc caggtatgat
    22021 aggtgcatat ttagcaaaag aagtaaatgc aggttttata ggtggtatca tagcaggatt
    22081 tatagcagga tttgttgttt tacaattaaa gaaaattaaa ttagctccta caatgagaac
    22141 tttaggttct atatttattt atcctttagt tggtacattg attacaggtg gtataattgt
    22201 atttttaatt ggagaaccta tagcttcttt tatgacatgg atgacaaact ggttaaatgg
    22261 aatgtctggt gtttctaaaa ttcctttagg tggaatctta ggaggaatga tagcctcaga
    22321 tatgggtggc cctataaata aggttgctgc tacttttgcc caaactcaag ttgacacact
    22381 tccatattta atgggtggag ttggcgttgc aatttgtata cctccaattg gtttaggatt
    22441 agctacatta ttgtttccaa aaaaatttag caaggaagaa agagattcag gtaaagcttc
    22501 actattaatg ggatgtgttg gaattacaga aggagctatt ccatttgcaa catcagatcc
    22561 agttagagta ataccttgta ttatggctgg gtcgattgta ggaaatatta tggctttttt
    22621 attaggatgt ttaaatcatg caccctgggg aggtctgata gtgttacctg tagttgacaa
    22681 taggttgggg tatattgcat cagtattaac tggagcagta gttgttgcgg tattaatgaa
    22741 attagtaaaa aaagacgtga aagaagatga agaaattgaa gaagatttag atgaatcaat
    22801 tgaattaata tttgaagaat tataaatata aatggaagga atttgtataa aaaagtaaat
    22861 ataaaactat aaatttaaag gagataagaa tatgaaaatt gtagcagtaa catcttgtcc
    22921 tagtggagta gcacatactt acatgtcggc tgaatcactt gaattatcag ccaaaaaatt
    22981 tggtatagaa ataaaggttg agactcaagg aagctctgga atagataatg aattgtcact
    23041 taaagatatt gatgaagcaa cttgtgtaat attgactaat gatgttgaga ttagaaatat
    23101 ggaacgtttt aaaggaaaga aggtagtaag aatgagtgta tctgagataa ttaagaaatc
    23161 tgatgcatta attaagaaaa taaaagatac ttttcaataa aaattgattt attttacata
    23221 taactcaaag atgtttttaa agaaattttt attaaaagca tctttgaaaa tatcttaaaa
    23281 atagaataaa aggagatata gtcatgaaaa agttaccaat aaagactgtc gtgcaaagat
    23341 tactaaactt acaagaagag gggaaaagtg caactctttt aggaattggt cctatgtcac
    23401 caaacttgtt acaagcaagt tttgaactag caaaagatga tgattttcct ttaatgttta
    23461 tcgcaagtag aaatcaagta gatgcagatg aactaggtgg cggttatgta aatggttgga
    23521 atcaggaaac ttttacaaaa gatattaaga aggttgccga caaagttgga tttgatggtt
    23581 tatattattt atgtagagac catggtgggc catggcaaag agataaagag agaaatgacc
    23641 atttgcctgt tgatgaagca atggagttag gaaaaaaatc ttatttagcg gacattgaag
    23701 caggttttga cctattgatg attgacccta caaaagaccc atttgaaatt ggaaaagtaa
    23761 ttccactaga tgtagtacta gaaagaactg tagaattaat tgagtattgt gaaaacgaga
    23821 gaaaaagact taatctacca gacataggtt atgaggttgg tacagaggaa accaatggag
    23881 gtctgacttc aactgagacg tatgaaacat ttataactag actaaaggtt gaactcgata
    23941 atagaggttt gccaatgcca acatttatag ttggtcaaac tggtacttta actagaaaga
    24001 cagaacaagt aggaactttt aattttagaa atgcttatga tttagctcaa atggctaaga
    24061 aatatggagt tggtttaaaa gagcataatg gcgattactt agatgatgta actttattag
    24121 aacatatacc atctcaaatt attgctacaa atgtagctcc tcaatatggt actgaagaaa
    24181 ctagggcata tttaaaatta gcagaagttg agtgtaaatt agaaaaagag ggcttagttg
    24241 agaaaacttc taatatcaga catgttcttc ttgttaatgc aattgaatgt ggacgttgga
    24301 gaaaatgggt agtaggagaa caaaaaaatc taactactga agagatattt aaggatgaag
    24361 ttttatctaa tgaaatacta gatattgcag gacattatac atttaataat gatgatgtta
    24421 aaaaagaaat agaagtgctt tatgataatt taagtaagaa taatattgat ggtcaaagat
    24481 ttgttgtaga ccatattaaa cgtcctttgc gtgattatgc agaatgctat aacttaaaag
    24541 gtgttactac gaggatttta aataaataat aggacttata gtggtgagta ttgttatact
    24601 aggtatttat agttataatt atacttacca ctattaaatt taggaggact aatattttga
    24661 ttaatttaat ttttgatgta gatgatactt tatataatca attaacgcct ttttacactg
    24721 catataataa agtgttttct tcaataaagg atatttctat tgaagattta tatatgagta
    24781 gtagaaagta tagtgatgag gtatttcata tgactgaaaa tggagaaatg ccaatcaaag
    24841 aaatgcatat ttataggata atgaaagctt ttgaggagtt gggaaattct attacagaaa
    24901 aagatgcaca aagttttcaa gatgagtata tatatcaaca atcacaaatt acactaatac
    24961 cagaagttga gaggatttta aatttttcta aagaaagaaa tattaatcta ggaattataa
    25021 ctaatggccc atcaaaccat caaagaatga agttaaagca gttaaatatt gaaaactggg
    25081 ttgataaaag taatattttt atttctagtg aggttggatt ttctaagcca gatactaata
    25141 tttttagagt agctgagaat gttatgaatt tagatagaga aaatacatat tatgtgggtg
    25201 attcttatag aaatgatgta ttaggtgcta aaaaagctgg ttggaagagt atatggttga
    25261 accatagagg acatgaggta gaagagttgt tttacaaacc tgattttgta attttggaac
    25321 ataaggattt gatatcttta tttataaaaa tatgtagtta taaaaatatg tagtaaaaaa
    25381 atgttaataa tagaattgta taaaataatt aataataact atataaaatt atatgtttag
    25441 attgtgagca atctatttta tgttaaaaat agattattta gaagaagggg aaatcttaaa
    25501 aaaatttgaa tatccccttt ttattattta attaaaatga aatagttaga aatatatttt
    25561 atttagttta gtcaataaca ttttgggatt ctttataact acatgttaac tgttgaatag
    25621 gtttatcata tggtttaacc catcctttat taatacttaa ttctgtaagt gctgtgtgac
    25681 cttctaccat ttgagttaat gaagctgaat atatagctct taattcaggt gtactacttg
    25741 tcaatgcaga attaagatac atattggcag atgctttagc agatgaaagc atacttaaca
    25801 ttattacttt atcgtctata taagtattgt tcttaattag attttctatc atatttctca
    25861 tgttatctat acctcctcag aaattagtat ttcattttca actataaatt gttgcatagc
    25921 tttaattctt ccttctgttg ctaatatact agattctgat tgtcttttta ggtcttcatc
    25981 tgatattaac atatttattg ctctttgcat aagtaatccg tcagactcca tttttataac
    26041 tgctgctaaa gatatttttt cagtttctga aagtattctc atttaatcat ctcctatatt
    26101 gtaatttatg ctttaatagt atgtctttct tttcaaggaa ataaccatta cactttttta
    26161 cagtatttta tattctcttt atatcaaaat ttagtcgacc taatatatta ataataattt
    26221 tgtaggatat ttatcattaa atatctataa aataaattat ttgaatattc ataaagcaaa
    26281 atttatttgt atgtacttct tcaagagtca atatgagttt tttcaaaaaa ttaattttta
    26341 tgataatcat taagggctat atttttctta aagttgtgat tatcattatt aatttacaat
    26401 aaaattgccg aaaagccctc tcctttaggt gtagggatga aaggtaaggt cttaatataa
    26461 aaaaagttag aataatgctt gtaatctaat accgctactg ataccctata tttaggaggt
    26521 gttagttttt atgtataaat ctaacaaaaa tatagtttat gattgcaaat atcatattgt
    26581 attttgccct aagtatcgta aacctgtttt agttggcgaa gttgaaaaga tgttaaaaga
    26641 aatactacca tataaagcag atgaacttgg agcagaaata atagaaatgg aaactgataa
    26701 agaacatgtg cacttattaa tatcttgtga ccctcaatat ggaattcata aagtagtaaa
    26761 aggtttaaaa ggctttagtt cgagagtatt aagagagaac tttccacatt taaagtctag
    26821 tatgcctagc atgtggacta atagctattt tgtaggtact gttggttctg tgagtttaga
    26881 agtagttaaa caatatatag aaaatcaaca agttaggaca aagaaatgat aaaaactcat
    26941 agagtgaaat taaacctaac tagaacacaa tttgaattag taagggaaaa gcaaatggaa
    27001 agtgctaact gttggaacta catagttaac ttatctaaag aatactattt tgaacataaa
    27061 caatggatta aaaagaatga tatacaaaag ttaatcaaag gtaagtacaa tcttcatagt
    27121 caaactattc aagctatatc agataaattt gatgcaaata gaaagactat atcagaatta
    27181 agaaaaaaag gtaatactaa agcaaaatat ccatacaaga ctaagaaatt ttatataata
    27241 ccttttaaag ctagtgctat taatagaaat gctaaaggca acttaaaatt atcaatgtca
    27301 aaaggcaggt atttagaatt agattttaat gttgagaata taaaaactgc tgaaatagta
    27361 tggcgaaatg gatattattt atactacact tttgacaatg aattaaatgg agttgtagcc
    27421 aaaggcacta ataccgtagg tgtagactta ggggaaatac attcgatagc tagtgtaaca
    27481 aatgaaggtg tagggcttat tctatctaat agagaaggca gaagtattaa acaatttaga
    27541 aataagatgt atgcttatat atctaaaaga ttaagtaaat gtaaaaaagg tagtagacaa
    27601 tcaaagaagt tatggagatt aaaaaataag ataagaagta aaacagataa tcagttaatg
    27661 aatttatatc atcaaacaac tagaaagttt atagactttt gtgtagagca gaaggtatgt
    27721 gaaatagttc ttggggatat aaaaggggtt gaaaaagata ctaagaaaaa gaaaagatta
    27781 aacagagtta atagacagaa aatatctcaa atggagtatg gtcgaataaa ggactatata
    27841 aaatataaag caaaagagca aggtattgaa gttaagttag ttaaagaaaa ctacacttct
    27901 caaacttgcc caaagtgttc taaaaaacac aaaccaactg gtagaactta tagttgtact
    27961 tgtggttatg aaactcatag agatatagtt ggtgcttgga atattttaaa taaaaagcat
    28021 aaatatggtt tagtagattt tagaataaat cataagcaac caataaattt aaaagtatct
    28081 accgtttgag ccatgtgtgg gcgaataact ttaaggtagt agtggcgaag acaccgtccg
    28141 aagtagcttg gctttgtaac cacctctgtt gtactgttta agtataaata agtgtgttag
    28201 ccaccaagaa tccccttgct ttagctatgg ggagtgtgtc aataaaatac tttaatgaaa
    28261 aatagctaac tttaattgta ggatattctt aatatatttg aaataaataa aaatattgaa
    28321 ggatataata ttcaattggt atataaataa taaatgatag ttaaatttta ccttagaggc
    28381 taattaggtg tttagtcaaa gctaatgcct ttagaataca aatgtattct aaaggcattt
    28441 ttactatgta ttttcattta tattactaaa cattatctat ttttatatac atttctatgt
    28501 attttatttt catcaaatcg gtctataaac tcattttttt cttcactata tccaatagat
    28561 ataagtgcat aaggaactaa ttttaatggt aagtctaaag tttgtctaac tttatttacc
    28621 actttatttt taggaaatac tcctgcccaa caagcagcta gaccttgatt agtaacttct
    28681 aataataaat tttgtgtgca agcacctaaa tcttgtatcc atttaccagg ttttaagaat
    28741 ttatctctat ttccaagcac tactatacat aatggtgcaa attctatatg ctttgctcta
    28801 tgctgtgact ttgagagctg tttcagcagt tccttatcat ctactataat aaattcccaa
    28861 ggttgagcat tttttgatga aggtgcttgc atagctgatt ttagtatttc agttatttta
    28921 tcttcttcta tatttttatc tatatatgtt cttattgaac gtctattttt tataacttct
    28981 aacatacaga ttctccttta aataaaatta tttctattta tataatatca ttactttaaa
    29041 ttttagctat tacttatata aagaataaac tttgtgttgg ttttatctaa gtaaaagtaa
    29101 cattcagaac taaaaaacaa gactataaat ccaaagtatt aattaaaaaa gaagtaataa
    29161 aaaataatat acaagattta aaaaagagta ttgggcttaa aatttaatta gaaaaccaat
    29221 tttgagataa tctgtttttt attttaacaa aaaaacacaa ataaagttcg atacgtagcg
    29281 aatgaaaaaa ttacaagaaa tatttttttt aaaaaatttc aaaaaagagt tgataatata
    29341 tgtatattaa tataaaattt aaggtgtata attcgtataa taatacgaat gatttgtagt
    29401 gtaaaataac ttataaacgg agaggggaag tctaatgaaa gtagcagtag ttatgggttc
    29461 aaaatcagat tatccaaaat tagaagaagg gataaaactg cttgaaaaat atggaataga
    29521 agttgtagca agagcattat cagctcatag aacaccagaa caactttcaa tattcctaaa
    29581 agaaatagaa gatgatactg atgtaataat agcagcagca ggaaaagcag cacacttacc
    29641 aggtgtaata gcttcacaaa cattaattcc tgtaatagga ttacctatta aatcatcaac
    29701 tatggatgga ctagactcac ttttatcaat agttcaaatg ccaaaaggaa tcccagttgc
    29761 aacagttaca atagacttag gtttaaacgc agctttattg gcattacaaa taatgacttt
    29821 aaaataccca aaattaaaag aggatttgaa atcatataga gaagagatgg cacaaaaggt
    29881 gttagaagac gacaagaatt taagggggta gcagaaatgt tattatatga aggaaaagca
    29941 aaacaagtat attctacaga taatgaaaat gaatatgttg tttattataa agatgatgca
    30001 acagcattta atggagaaaa aaaagcagaa atatcttcaa aaggtatact aaataataag
    30061 atatcaacaa taatctttga aatgttaaaa gaaaataata taaatactca ctttataaaa
    30121 agtttatcag atagagaaat gttagttaaa aaagtagaga ttttaccatt agaagtaata
    30181 gtaagaaata ttgctgctgg ttctatctgt aaaagagttg gtcttgaaga aggtgtagtt
    30241 ttcgatgagc caatttttga aataagctac aaaaatgatg cttatggtga cccaatgcta
    30301 aatgatgatt atgcagtggc aatgaagtta gcaactagag aagagttaaa attcttaaga
    30361 gaagaaactt taaaaataaa tgagttacta aaagcatttt tcttaaaatt aaacctaaag
    30421 ttagttgact ttaaaataga atttggtaag gattctgaag gaaatataat attggcagat
    30481 gaagtatcac cagacacttg tagactttgg gatgtaaata caaatgaaaa gttagataag
    30541 gacagattta gaaaagacct tggagacctt gtagaaggtt atacggaagt actttcaaga
    30601 atgaataata aataggaggc aaatgtatgt gtggagtttt agggatttac tctaataaag
    30661 atgtaactaa agaactatat tattctttat attctatgca acacaggggg caagaaagct
    30721 gtggtttagc tcttttagat gatggagaaa ttaagtacaa aaaagatatg ggattagttg
    30781 gagatgtttt taaggaaaat gaattatcta agttaaaagg aaatataggg attggacatg
    30841 ttagatattc aacagctgga ggaagtcatg tttctaactg tcaaccttta gttggaagtt
    30901 gtagaaaaag acaattagcc atagctcata atggaaatct agttaatgca aattatttaa
    30961 aagatatgct agaagaagat ggatatatgt tccaaactaa ttcagataca gaagtgattt
    31021 tatatatact tgctagatat tacaaagggg atatagtaga aagtttaaaa gtgacaatgg
    31081 attatataaa aggtgcatat gctcttgtaa taatgagtca agaagaattg gttgcagtta
    31141 gagacccaca tggattcaga cctcttgtac ttggtaaaaa gggtgatgaa tatatatttg
    31201 catcagaaaa ttgtgcaata gatatacttg gcggagaagt tataagagat gtagagccag
    31261 gagagataat agtagtcaaa gatggagaac taaaatcata tttttattca gaaaattata
    31321 aaccagttaa gaaaagttgt atatttgaac acatatactt tgcaagaaat gatgcaacta
    31381 tagacaatgt aaatgcatat gaatttagaa ttaaatgtgg tgagagatta gctcagaatg
    31441 aaactgtaaa ggcagatatg gtagttccag ttccagattc aggttggcca ggagctattg
    31501 gttatgcaaa tgctagtgga ttaaaaataa gtgaaggctt agttaaaaat agatatgtag
    31561 gaagaacatt tataaaacct acacaagaag aaagagaaat agcagtaaaa ataaaattaa
    31621 atcctctttc aacaataata aaaggaaaat ctataatttt agtagacgac tcaattgtaa
    31681 gaggaactac atctaaacaa ttagtaaaat ctttaagaga agcaggagca aaagaaattc
    31741 atcttagaat aacttctcct ccagtagctt actcttgtta ctatggaata gatactccaa
    31801 atcgctctaa attaattgca tcaagtaaca acgtagaaga aatgagagaa tatattggat
    31861 gtgatagttt aaaattccta gatatagaag gaatgttaga tgcaacagaa cataagtcta
    31921 ctttctgtaa agcttgtttt gatggagaat acccagttaa gaaaattgat aaggaggaac
    31981 tactttcatg ttaacatata aagaatcagg tgtagatata gatgaaggaa atagagcagt
    32041 agaccttata aaaggtaaaa taaaaggaac ttatgatgga aatgtagttg gagatttagg
    32101 taacttcagt ggattataca gtttaaaaga ttttgtaggt atgaaagaac cagttttgct
    32161 tgcatctaca gatggtgttg ggactaagtt aaaaatagct cagatgatgg acaagcatga
    32221 tactgttgga atagatttag ttgcaatgtg cgtgaatgat ttaatttgtc aaggagcaaa
    32281 accattattc ttccttgact atatagctct tgggaaatta gtgcctgagc atatcgaaaa
    32341 aatagttggt ggaattgcag atggttgtaa aatgtctggt tgtgcactaa ttggtggaga
    32401 aactgctgaa atgcctggaa tgtatggaga agatgactat gatttagcag gattttctgt
    32461 aggaatagca gacaaagaaa agattgtttc tggaaacaat gttaaatcag gagatgtatt
    32521 ggttggtata tcttcaagtg gagtacacag caatggattt tcttttataa gaaagatatt
    32581 tttagaaact tacaattata aaatggaaca atatgttgaa gaactaggaa tgacagttgg
    32641 agaagcactt ttaacaccaa ctaaaatata tgttaaatta gctttagatg tattagctaa
    32701 acatgacata aaagctatag ctcacataac tggtggaggg cttatagaaa atataacaag
    32761 agttatacct aaagggttag gactagatat caataaaaaa tcttgggaga agcctcctat
    32821 atttaaaatg atagaaggtt ttaatgctgt tgatgagaga gaacttcata agagttttaa
    32881 catgggtatt gggctagttt taatagtaga taaagaaaat gctgatgatg ttgtaaactt
    32941 tataaataat agagaaaatg acaatgctgc ttatgtagat aaaaaatata gtgaattatt
    33001 agaagataaa gcttatataa ttggtgaagt agtagatagt catgaaggtg tagaattatg
    33061 ctaaatatag gagttttaat atctggtgga ggaacaaatt tacaagcagt tatagatggt
    33121 actgagtctg gagaaataaa aggtcaggtt aaagtagtta tctcaagtaa gcaaggtgct
    33181 tatgggctag agagagctaa aaatcataac ataaaagcaa tttgtgaaac tgatgaagac
    33241 aagataatag aaattcttaa ggaaaataaa atagatttag tagttttggc tggttattta
    33301 aaaataatca gcccaaagct ggttaacgag tttagaaaca aaatgataaa catacatcca
    33361 tcattaattc cttcattttg tggagctgga ttctatggag aaaaagttca tcaaggtgtt
    33421 atagattatg gagctaaagt aactggtgct acagttcatt ttgtagatga aggtgctgat
    33481 actggaccaa tcataatgca agatgtagtt aaggtaaatc aagatgatga tgcaaagaca
    33541 cttgcaaaaa gagtgttaga agttgaacat aggattttaa aagaaagtat aagtcttttt
    33601 tgtgaaaata aattaaaact tcaaggtagg agagtgttta taaatgagta agagagcttt
    33661 aataagtgta actgacaaga ctggtgtagt ggaatttgct aaagagttaa ataaattaga
    33721 ttatgaaatt atatctacag gaaatacatt taaaacatta aaagaaaatg gtgttaatgt
    33781 aatgcaagtt gaagatgtta caaattttcc agaaatatta gatggaagag taaaaacatt
    33841 aaatccatat atacatggtg gtatacttta taaaagagat aaagaatctc atgtagaaac
    33901 tgtaaatgaa cacaaaatac attcaataga tttagtagct gtaaatctat atgattttga
    33961 aggaacttta aaagctggaa aaagtcatga tgaaataatt gaaaatatag atattggtgg
    34021 accatctatg atacgttctg ctgccaaaaa ctataaggat gtaatagtag tagtagatat
    34081 aaaagattat gattctataa tagaaaaatt aaaaacagat acaatgacat tagaagacag
    34141 aaaaaaatta tcatataaag ctttctcaac tacaggaaga tatgatgcgt taatatctag
    34201 ttattttgct ggagaagttg gagatacgta tcctgacata ttaaatttaa ctttccaaaa
    34261 agaacaaact ttaagatatg gagaaaatcc acatcaaaat ggtttcttat attcacagtc
    34321 aaatgcaaaa aatccaatct taaattatga gcaattaggc ggtaaagaac tttcttttaa
    34381 caacttaaat gatttacatg gatgtttaga agtaatgaga gaatttaaag atagtgaaga
    34441 agtagtttca gtagctataa aacatgctaa ttcatgtggg gtaggtttag gaaaagatgc
    34501 ttttgaagca tatacaaaat gttatgaagc ggataaggtt tcaatatttg gaggaatagt
    34561 aggaataact tctactatag ataaagctac agcagagaaa ttaaatgaaa tattcttaga
    34621 aatagtagta gcatatgatt ttgaaccaga agctttagag atacttaaac aaaagaaaaa
    34681 tctaagaata ttaaaattag ctaaaataga aaatagttta caaccatatg aaatgaaata
    34741 tttagatgga aaattattga tacaagatag aaacaatata ttagcagaaa aaagtgaaaa
    34801 tgtaacaaag gaaaaaccaa cagatgcaca acttaaggac atggaatttg gtatgagagt
    34861 agtaaaaaat atgaaatcaa atgcaattgc agtagtaaaa aatggacaaa ctctagcttt
    34921 aggttgtgga caaacatcaa gaatttgggc attaaaaaat gctcttgaaa ataacaaaga
    34981 taaagatttt acaggagctg ttttggcgtc agacgcattt ttcccatttg atgattgtgt
    35041 aacattagct catgaatatg gaatatcagc agtagttcaa ccaggtggct ctataaaaga
    35101 taaagattca atagaagctt gtgacaagta tgatatggtt atggtattta caggaataag
    35161 acacttcaaa cactaaggag gatattatga agatattggt tgtaggcggt ggcggaagag
    35221 agcatgctat atgctggaaa ctaagcaaag aaaaaaatgt agaaaaaatt tattgtgcac
    35281 caggtaatgc aggaattgca aacgtagcag aatgtgtaaa tataggtgat actaatatag
    35341 aagaactttt aaaatttgcc aaagaaaatg aaataggtct gacaatagta ggaccagaag
    35401 taccactagt tatgggaata gtagatgagt ttgaaaaaga aggattaaga gtatttggac
    35461 ctaataaaaa atgtgctcaa cttgaaggaa gtaaagcttt ttcaaaagaa tttatgataa
    35521 aacataacat acctactgca aaatataaag aatatacaaa cttagaagaa gctatatcag
    35581 aaatagatag ttttggatat ccagtagtaa taaaagcgga cggattagct gctggaaaag
    35641 gtgttgtaat acctgaaaat agagaagatg ctatagcaac tttaaaagaa atgatgtcag
    35701 ataagaagtt tggagctgct ggagataaaa tagtaattga agagttttta aaaggaatag
    35761 aaacttcaat tctagcattt gtagacaatg atacaatagt tcctatggca agtgcaaaag
    35821 accataaaaa agtaaacaac tatgagcaag gtccaaacac tggaggaatg ggaacttttt
    35881 cacctagtga aatatataca gaagaattag caaataaagt aaaagagaca gtattagaaa
    35941 aaactttaga aggtttcaaa aaagatggac ttaattttaa aggtatttta tttgttggtc
    36001 ttatgataac tgaagatgga gaaaaagtac ttgaatataa tgtaagattt ggagaccctg
    36061 agacacaatc tgttttattt agattagaaa cagatttaca tgagataatg gaagctatac
    36121 tagataataa gttaaaagat atagaaataa actatagtga tgatgaagct gtatgtgtta
    36181 tgcttacatc aggagggtat ccagacagtt atgaaaaagg gaaaattata actggtcttg
    36241 aaaacttgga tgatgatata gtagtattcc atagtggcac taagatgttt gatggtaatt
    36301 tggttaccaa tggaggaaga gtaattggta taactgcaaa gtcaactaga gttaaggatg
    36361 cagcagaaaa agtttatgaa aacataaaga agataaactt tgaaggaatg cactatagaa
    36421 cagacatagg aagataattt ttctaaaatg gaggataagg tatgttgaat actgaaaata
    36481 aagactcaat ggttagaaga gtattggttg aaaaaagaga aggttttgac cttgaggcta
    36541 aagctttaaa aaaagactta gtagaaagtt tacacataga taatatagaa aatttaagaa
    36601 tattaaatag atatgatgta gaaggaatat cagaagaagt ttatgaaaat gcagcaaaga
    36661 caatattctc tgaaccaaat ttagatgtag tatattatga agaaatacca aaattaaatg
    36721 atgaaagagt atttgcaata gaatttcttc ctggtcaata cgaccaaaga ggtgattggg
    36781 cagctcaatg tgtacaaatt gtaaatcaag ggataagacc tgctattaat acagcaaaag
    36841 tttatatatt atcaggcaaa ataactgatg aagagttttc taaaataaaa gattactgta
    36901 taaatccagt agatagtaga gaagcatctt tagagaaacc agaaacgtta aaaatggaaa
    36961 ctgaaatacc aacaacagtt gaagtattag atggatttat agatttagat gaaaatggat
    37021 taagaacatt tgtaagtgaa aaaggattag caatgacttt aggagactta caacacgttc
    37081 aaaaatactt taaagataca gaaaagagaa atccaactat aacagaaata aaagtattag
    37141 atacttattg gtctgatcat tgtagacata ctacttttat gactgaaata gaaaatgtaa
    37201 agatagaaga tggtaagttt aatgatatag taaaagaagc gtatcaaatg tatcttaact
    37261 caagagacaa tgtatatgtg aacagacata aagatatatg tttaatggat atagccactg
    37321 ttgcagtaaa agaattaaag aaaaatggga aattaaatga cctagatgaa agtgaagaaa
    37381 taaatgcatg tagtataaac gtagatgttg aagttgatgg taagatggaa aaatatctag
    37441 taatgtttaa aaatgaaact cacaaccatc caactgaaat agaaccattt ggtggagcag
    37501 ctacttgttt aggaggagct ataagagacc cattatcagg aagaagttat gtttatcaag
    37561 caatgcgtgt tacaggaagt gctgacccaa gaacgacttt agaagatact ttacctggaa
    37621 aacttatgca aagaaaaata actacagaag ctgctcatgg atatagttca tatggtaacc
    37681 aaataggtct tacaactgga caagttgcag aagtatatga tgaaaacttt gttgcaaaga
    37741 gaatggaaat aggtgcagtt atagcagcag caccaaaaga gaatgtagta agagaaagac
    37801 ctgaagcagg agatgtaatc gttcttcttg gaggaaaaac aggaagagat ggctgtggag
    37861 gagcaactgg ttcatctaaa gaacatagtg aagagtcaat cttaacttgt agtgcagaag
    37921 ttcaaaaagg agatgctcct aacgaaagaa aaatacaaag attctttaga aataaagaag
    37981 ttgctcaaat gataaaaaga tgtaatgatt ttggagcagg tggagtttgt gttgctatag
    38041 gagagattgc tgatagctta gatataaact tagacttagt tcctaaaaaa tatgatggac
    38101 ttgatggaac tgagcttgca atttcagagt cacaagagcg tatggcagta gccataaaaa
    38161 aagaaaataa agataaattt atacaattag cagtagaaga aaacttagaa gctacgcatg
    38221 tagctacagt aacagataca ggatatttaa gaatgttctg gaatggaaaa gcaattgtag
    38281 atataaatag agagttttta gatacaaacg gagtaaaaca aactactgat gttcatgtta
    38341 ctaaagtaga tgaagaaaat acattcttta gctctaatga aatagtaaaa gatgttaagt
    38401 gtgcaagtat gaaagacaag tttacaaaag tactatctga ccttaatgtt tgttctcaaa
    38461 aaggattagt agagatgttt gataatacaa ttggtggtaa tacagtactt atgccatttg
    38521 gaggaaagta tcaagcgact ccaacacaag gtatggttgc taagatacca gtacttggag
    38581 gagaaactaa tacttctaca ataatgactt atggatacaa tccaaaggta ggaaaatgga
    38641 gcccattcca tggtgccttg tatgcagtag tagaatctgt gtgtaaattg gttgctattg
    38701 gaggaaatta tagtacaaca agacttacat tccaagagta ttttgaaaaa cttggtaaca
    38761 atccagaaaa atggggtaaa ccattctcag cacttttagg agcgttttat gcacaaagta
    38821 aatttgagat acctgctata ggtggaaaag acagtatgtc tggtacattt aaagatattg
    38881 aagttccacc aacattagtt tcatttgctg ttgacacagt agatgcgaag aaagtagtat
    38941 ctccagaatt taaaaaagca gactcaaaag tagtaatgtt atgtgtaaac aaggcagaaa
    39001 atgatgttgt agattttgag gaattaaaga gaaacttaga taaagtaaga gaacttatac
    39061 atggaaataa agttttatca acatatgcat taggatttgc aggtgtaggt gaagcaataa
    39121 gtaaaatggc ttttggtaat aaaataggat ttaagtttag tgaagaagct gaaaaagcat
    39181 ttacagataa taaattattt gaggctagtt atggaaatat agtacttgaa ttagcaaatg
    39241 atgatttaag tatgttagaa ggatacaatt atgttgtgat tggaagtaca gttaaagaag
    39301 ctagtatatt tatcaaaggt gaagaacttg cattagatga attatataaa gcacattgta
    39361 gtactttaga gcctatattc ccaactaaaa cagaagaagt aaaatcaaag atagaaacta
    39421 taagttatat atcacaagga gaagctaaaa aatcatcatt aagtatagct actccaagag
    39481 tatttatacc agcattccct ggaacaaact gtgaatatga ctctgcaaga gcatttgaaa
    39541 gagcaggagc taatgcaagt ataagagtat ttaagaattt aacttataaa gacatagaag
    39601 attctataga tacaatagta aacgaaataa aatcttctca aataataatg ttaccaggtg
    39661 gatttagtgc tggtgatgag ccagatggtt ctggtaaatt tatagctact gtatttagaa
    39721 atcctagagt gcaagaagct ataaatgaat ttttaactca aaaagatgga ctaatgttag
    39781 gtatctgtaa tggattccaa gtattaatta aattagggtt agttccatat ggagaaataa
    39841 gagttccaag tgaaagtgct ccaacactta cttacaataa tattggtcgc catcaagcta
    39901 agattgctag aactagaatt tcatctaata aatctccttg gttagctcaa actaatgttg
    39961 gagatattca taatatagca atttctcatg gagaaggtaa gtttgttgcc agtgaagatg
    40021 ttatgagaga attaattgct aatggacaag tagcaactca atatgttgac tttaataatg
    40081 aagctactta tgatattgag tttaatccaa atggttcatt ctatgctgtt gaaggtataa
    40141 ctagtgctga tggtagagtg tttggtaaaa tgggacattc tgaaagaatt ggtgaggaag
    40201 tatataagaa tattataggt gaaaaagacc aaaagatatt tgaatctgga gttaaatatt
    40261 ttagataaag ctttgatgtt taattgatag ctagaatata taatttagat atttagattt
    40321 aaagagatgt aataatatga tgataatcat ttaatattac atctcttttt tatttatcta
    40381 agaaataata agttcaacct aagttaaaat acaagctgtg ttataattaa ttattaagta
    40441 aaattgaata tttattggag ggcaacataa ttaatgaaaa tattaataac agggtctaat
    40501 ggtcaattag gaaaagaatt ggttaaccaa ttagaagcaa tcaatcaaag tataaaccaa
    40561 ccaaaatatg ttatattagc tacaacaaga agtgatttgg acatatccaa tcagactaat
    40621 gtagataatt ttattttgca taataaacct gatgtagttg taaactgtgc agcatataca
    40681 aaggttgatg catgtgaaga taatatcgaa attgcataca aaataaatgc tttaggagtg
    40741 agaaatctag caatagcatc agaaaaagtt aatgctaagc ttattcacat atcaactgac
    40801 tatgtattca atggtttttc taaatatcca tatagggaag acaataaaac tgaacccaat
    40861 tctgtatatg gaaaaagtaa acttatggga gaaaaatttg ttgaacaatt ctcacataag
    40921 tactttatat tgagaacagc atggttatat ggtaatggga ataattttgt aaagaccatg
    40981 ataaaacttt ctttagaaaa taaagaggtt aatgtggtta atgaccagtt tggaactcca
    41041 acaagtacag tagacttagc aaaaattata ataagaatca tggaaacaga acattatgga
    41101 gtttatcatg ctacatgtga aggtgagtgt agttggtatg attttgccaa aaagatattt
    41161 aaactaaaaa atatagatat aaaagtaaat ccaattaaga gtaacgaatt taaatgtaag
    41221 gctccaagac cactatattc tgtattggat aattttatgt tgaagttaat tggtttaaat
    41281 tcttttagaa agtgggaaga atccattgaa gaatatttag aaagatatga tataaatgaa
    41341 aagtaaatgt attattttga gaaaagaaaa taagatatta taaaaaatat aaatatttct
    41401 tttatttatc cgataatcat aatatgacaa aaacaaattt aaggatggaa aaagatgaaa
    41461 ggaattattt tagcaggagg gtctggtaca agactatatc ctataacaaa atgtatatca
    41521 aaacaaacac ttccaatata cgataaacca atgatatatt accctatgtc ggttcttatg
    41581 ttggcaggga tacaagatat attaataata tctacaccaa gagatataaa agcatttgaa
    41641 gaattatttg gagatggaag ttcactaggg cttaacataa catatgcaat tcaggagaaa
    41701 gcaaatggaa ttgcagaagc gtttataata ggagaagaat ttataggaaa tgacagagtg
    41761 tctcttgtat taggggataa tatattttat ggatatggat ttagtgaaag gttggagaat
    41821 gcagttaaaa gaaaaggagc aactatattt gggtatcatg taagtaatcc aaattcgttt
    41881 ggagttgtag aatttgataa ggattttaat gtattgtcaa tagaagaaaa acctacagtt
    41941 cctaaatcta attatgctat accaggatta tatttttatg ataatgatgt tgtagagata
    42001 gcaaagctag ttgaaccgtc agatagaggt gaattagaaa taacctctat aaacagtgaa
    42061 tatctaaaac gaggaaaact aaaagtggaa cttctaggaa gaggtatggc ttggcttgat
    42121 actggtacac ataaaggtct attggatgct tctaattatg ttgaagtggt tcaaacaaga
    42181 cagggactgt atatagcatg tttagaagag attgcatata caaaaggata tataagtaga
    42241 gaacaattaa tgaaactagc caagccactg ataaaaactg aatatggtca atatctatta
    42301 aaattggcta gtgaagatat gtaatatggt agataaaatt tattataaat tttattaagg
    42361 gagtttatta taatgggaaa aatcaatgtt ataaagacta aaatagaagg attatatata
    42421 gtcgaaccaa atgtatttta tgatgaaaga ggatatttta tggaaagcta taataaaaag
    42481 gattttatag agcagggtct tgatatgtgt tttattcaag ataatgagtc tagctcaaga
    42541 aaaggcgttt taagaggaat gcattttcag gttaaatatc ctcaaggaaa attagtcaga
    42601 gttacagagg gagaagtttt cgatgttgca gttgatttaa ggaaagattc acaaacattt
    42661 gggaaatggg aaggtgtaat attgtctgat aagaataaaa aacaatttta tattccagaa
    42721 gggtttgcac atggattttt agttttatca gaaaaagcag tatttagtta caaatgtaca
    42781 aattattatt acccagaatt tgagaatggc tttatatgga atgacaagca aatatctatt
    42841 gagtggcctt taaaaggaat tgaagaaata tttttatcag aaaaggacaa aaaacaaaaa
    42901 agttttaaag atatttgtac aagacgttaa taaaaattta ttagaattaa catttaggag
    42961 aaaaaatatg aaaaaaatac tgataactgg aggagcagga tttattggga gtaattttat
    43021 acattatatg cttaacaagt atgtagatta ttttattgtt aacctagact tgttgactta
    43081 tgctggtaat ttagagacat taatagaaat acaagacaat tccaattata aatttgttaa
    43141 aggagatatt tcaaataggg agcaggtgta taagctattt gaagaagaaa ggtttgatat
    43201 agttgttaac tttgccgctg aatctcatgt agataggtca gttgagaatc ctgatttatt
    43261 tattaaaaca aacattatag gaacacaagt acttttagat gcatctataa aatatggagt
    43321 taaaaggtat catcaaatat ctacagacga agtgtatggt gatttaccaa ttgatagaaa
    43381 ggatttattc tttacagaac aatcttctat caatccaagt tctccatatt cagcttctaa
    43441 agcatcagca gatttattgg taagttctta ttatagaaca tatggattgt taacaactat
    43501 atctaggtgt tccaacaatt atgggccata tcattttcct gaaaaattga ttcctcttat
    43561 gattttaaat gctttggaaa acaaacaatt gccagtatat gggaatgggg aaaatgtaag
    43621 agattggtta catgtatatg accattgtac tgctatagac ttaataatac ataaaggaaa
    43681 tataggggag atttacaata taggaggtca taatgaaaga agtaatctag aagtagtaaa
    43741 aatgatatta aatcttttag gtaaatctga agagttaata tcctatgtaa atgatagacc
    43801 tggtcatgat ttaagatatg ctattgatgc aactaaaata gagaatgagt taggttggaa
    43861 ggcaaaatat gactttgatt tgggtataaa agaaactgtt aagtggtata tagaaaatga
    43921 atcttggtgg aaagctgttt tatcagtata actttaaaat ttagaaaggg gcgttttata
    43981 ttgaatgaga taagttcact aattaatata ttagcattga ctcaattatc tggtagtaat
    44041 actacaaatc aatgtaattg tacatataat aattcatctg gatttgatat gattatgatg
    44101 accttgttaa aagcattatc acaaaataat caacatactt ctaatagcta caatttattg
    44161 tctaattctg agaatttatt taatgaactt gataatgtag taaataatac cactagtaga
    44221 tttatagatg ttgatacaaa agataaaaat gtaaagttga gaattgaaaa tgctgtagat
    44281 caagcatcaa aaaaatataa cgtagattca aatcttataa aagcaataat taaagttgaa
    44341 tcagatttta atccaaatac cgtttcttct gctggtgcta agggtcttat gcaacttatg
    44401 ccagaaaact gcagagactt aggagttaca gaccctttta atatagaaca gaatatagat
    44461 gctggaacta gacatattaa ggaatacatt gatatgtttg gagggagcat agaaatggga
    44521 ttgatggcat ataatggtgg tcctggaaga atgaaaagta gaggagtaga atcaatctct
    44581 gacctgtata aaatgccaaa agaaacacaa aattatattc ctaaggttat gaaatattat
    44641 agagggtagg ctttattatg ggaataaata tagataagct aaacataagt ccaagtataa
    44701 atatggaggt taaaaaacta aaagagagta ataaattttc tgacaaatta aactctttaa
    44761 aacaatcttc ttcattggaa gatataaaac gaaatttgga aagtgtaaaa aaggcaggag
    44821 aaaggcttgt acttactcaa aactatggcg atattagtat atataaaaat gcagtaaaag
    44881 aatacctaaa atctgttgta gataatatgt attcacttga taaaaatagt agcttttggg
    44941 agcatcagta ttataagaat gtaaagattg ttgatagtaa acttgaggat atgactagta
    45001 agttaattag tgaagaaaaa gaaaacatag atattgtttc tactgttgac atgatacaag
    45061 gcttattgat agatatgtat agataaggtt atacaaggag aaaaatttta tgaatagcaa
    45121 aaaagaggat atatatgaat tgaattttgg gcaattaata gaagaaaaat ctggtgttat
    45181 agaagaaaat caaaatgttt ttaatgcaaa agtaaatgta gctgtttcta taggaaatac
    45241 aacagaaaca ataaaaaata tattagattt aaaagaaggc tctataataa aattaagtaa
    45301 gaatgtggat gaaaaattag atatatatgc aaacgataga ttgtttgctt atggagaaag
    45361 cattattaca aatgagaggt tatctgtaag attatctaaa atagaagatc cagatgtaaa
    45421 ttagcatttt aaatagtatc cctttaggct attctgtatg agtttagcta aggggatatt
    45481 tttataaaaa atttaaaata tgtgtgaatt atataaatat ttttatttta tgagcgataa
    45541 ctaaattata agtatgtaat tttggaggtg aaactatgga tattaaaaat gtaagttcaa
    45601 atattgtagt gaataattat ttaaagaata catctggtat aaaaaaagtg gaagaggtta
    45661 aaaaaattaa taaaacagat aagatagagt tatctaaatt tgcaaaggaa atggcaagtg
    45721 tgtcaaatat gagtgatgag aatattagaa ataaaaaagt cgatgaaata aaaaatgcat
    45781 tagaaaatgg aacttacaaa ataaaaccaa aagaaattgc gaaaaaaatg atagaggata
    45841 tgcgaggata aagtcatgat tacagactta aaagttataa tatatgaaga aaaaaatata
    45901 attagtaaga tgatggaatt attaaaagaa caatttgatt atatagttga aaaagactta
    45961 gaaaatttaa atatgttaaa tcctaaacta gaagatatta gcagggagct tgcatccata
    46021 gaaataaaga gaagacaact ttttggtgat gatatttcaa tgagtgaagt tgttgaaaat
    46081 agtaatgatg gatacttaaa agaaatatat acagacttga aacacattct tgctttagct
    46141 aaaaatcaac aagaatcaaa tgattctcta ataaagaaag aacttatttt tacgaaaaaa
    46201 atgattaatt tcataaaacc agctgataag caaactacaa cttataattc ttatggaaat
    46261 attagaaaat aaatattagg aggagaaata tggcaggatt attttccatt ttaaatacag
    46321 ctaattcagg aatgaatgcg caccaaaaag caatacagac aatttctcat aatataagta
    46381 atctagatac agacggttat tctaggcaaa ggaccgagtt tgccacaaat agtccaatgt
    46441 atatgcctag tttgagtaat tcaattgcaa gaggacaatt agggactgga gtacatgtaa
    46501 cggatgtaac aagagctaga aatagttttt atgattacca atttagggca gaagctcata
    46561 agtatggtaa gacagtttca aaatatgatt attacaatac tatagaaact atactaaatg
    46621 aaccatcaga ctatggaata tctgcaggta tagatgattt ttttaaagga tgggagtcat
    46681 tatcaaagga tataaatagt gacagtaaaa aaagtctagt ggtagaaaac gcatctaatt
    46741 tagcaacttt aataagtgaa agttataaga agcttgataa gttaaaagaa gatatagatt
    46801 ctaatataga cactgagata aaacaaataa atgacatgtt aaaaagtctt gaagatttaa
    46861 ataaatctat tgatattata tctggttctg gctctacacc aaatgattta cttgatgaaa
    46921 gagatagaat tttagataac ttgagtttta aactagactt tgaaaatagt gatgttaaaa
    46981 atatgcttgc agatggtaaa ttagaactta atgaattaaa aaatccagat ggaacttgga
    47041 aaacaggtat atcagggacc ttacaaggct tgtttgaaat gcatggtaag attgatacat
    47101 ataaaagcga tttaaaagat gtatctgatg gattagctaa acagataaat gatgtttata
    47161 atacgtctgc aggaataaca gtaacagatt tttttataac ttcaaatgta gatggtgaag
    47221 atattataaa gataaatcca gctataaaga gtaacccaaa tgaattaaag cttactgctg
    47281 atgaagcttc aaaaattgct aagttaaagg atgaaaaaat agatattggg gtggctggtg
    47341 gaaaagtaag tacaataagt gaccactata aagcctttgc agaatcaatc ggtttagatt
    47401 ctcaaaaagt aaatcaagat gaagcaaatc aacgtaaaat tataaataat attgataact
    47461 caagaatgag tgtttcaggt gtatctttgg atgaagaaat gacagagctt ataaaaatac
    47521 aacgttcata tcaagctagt gcaaaagtta tgtcaacagc tgtgcaacta ctggatgttg
    47581 taataaatgg aataatttaa tagaaggggg aatttataat gagagtcagt acaggtatga
    47641 tgtctagcag ctatctaaac tcactacaag ataatctaca aagattggat aaggttaata
    47701 gacaagttaa tacaacaaaa gaaataaaca agctatcaga taatccatat aaagcaataa
    47761 agatattaaa ctcaaaaagt gaaataaaga ctatggagac ttatgttgag aattgtaaag
    47821 atacagggaa ttggttagaa actactgata ctgcattaga tcagcttgga aaattattat
    47881 cagacataaa gaaaactttg gtggcaagtg gtgatggtgc atactcagat gatgaattaa
    47941 agactcttag taatgaaact aatgaaaaaa tgaaagaaat tgcaaatgct ttaaatgcaa
    48001 cacatgaagg gaagtacata ttttcaggtt caaatacggg ggtaccacct gttgaatgta
    48061 tagataatgc tgatggttca gtgagtttaa agtttaattc tagtctaaat cttaataaat
    48121 taaatgactc tttgagtatg gatatagctc aaggaataag tgtagattat aacgttaaat
    48181 tgagtagcct tggatttgac ccaacaaaaa ctgacccatt tgaagagtta aataacatat
    48241 caaaaaaatt agcaaatcca gatgctgatg ctataaaaga gcttacaaca acttgtttgg
    48301 gcgatatgga acaactaata gaaaatacag taaatgttag gtctatatat gggacaaaag
    48361 caaatacagt agaggctatg aaggaaaaaa acgatgatag tttgatacag ttaaaggata
    48421 ttttgtcaca aaatgaagaa atagattatg gtgaaaaact tgttcaatta aaggcagcag
    48481 agttaactta tcaagcttca ctacaaacag gaggaaagtt atttaatgta tctatattag
    48541 attacatata ataaaataac gcagatatga tgaaggttac attaaaaaaa ggtatattag
    48601 gttttgaaaa tttaaaagaa tatgagcttt tagatataga agataatgat tatttaaaag
    48661 aatttaattc tactgaagaa gaaggtatag gatttgttgt tgtatctcca tttgagatta
    48721 tcaatgaata tgaaatagtt ttaaatcagg aaacaataga aaaactagaa gtgaaatctc
    48781 caaaggatgt tgtattattt aatataatta ctcttggtca gacatttgaa gaatcaactg
    48841 tgaacatgaa agctcctata gtgataaata taaaaactaa ttttggaatg caaataattt
    48901 tacaggatga taaatattca ataaggcatc cattattgag aggtgatagt ggatgctagt
    48961 aatttcaaga aaaaaagatg aagcagtact tataggagaa agcatagaag taaaaattgt
    49021 aggggtagat ggaaacaatg tgaaactagc tatatcagcg cctaacaata taagtatatt
    49081 aagaaaagaa atatacgaaa aagtaaagag tgagaatata aaagctacaa ataaaaatat
    49141 aaaaatttta aagtcattaa aataataagc ttggaggaag taatatatgt atggagaaaa
    49201 tccttataat tcttataaac aaaatgctgt ttttatggca tcaaaggaac aattgttact
    49261 tatgcttgtt gatggagcag taaaatatac caaaatagcc aaaacagcta tcttagataa
    49321 aaatacacaa aaagcgcata gagagcttat aagagttcag gatatattta cagaactaat
    49381 ggttacatta gaccaaaatg caggtcaatg ggcaaaggat atgtacagag tttatgattt
    49441 tgtaagatat gaactttcaa gagctaatat tagaaaagac attcaaataa tagataatgt
    49501 gttgcctgta atagaacaaa ttaaagatac ttggcatgaa gctgataaaa aaagtaaaga
    49561 agaaagaagt aggtacaaat aatggaatta aatttagctg aattagataa gttatcaaaa
    49621 gaggaattac tattgatgct tgtagatgga acagtaaaat ataccaatat atccaaagaa
    49681 gctcttttaa ataacgacta tttgaaagca cataatgaac ttgtaagagt tcaaaatata
    49741 tttacagaac taatgactac attagaccaa gatgcaggtc aatgggcaaa ggatatgtat
    49801 aaagtttatg aatttataaa atctgaatta gcaatagctg atgaaaataa agatattaaa
    49861 ataattgatg atatattgcc aatagtaaaa caaattagag atacatggca tgaagtttac
    49921 aatcaactaa aaatataatt ggatggaggg ataaaggtat gtcaagtata agtcctataa
    49981 gagttacagg tctttcagga aattttgata tggaaggcat aatcgaagct agtatgatta
    50041 gagacaagga aaaagttgat aaagcaaaac aagaacaaca aatcgttaaa tggaagcaag
    50101 aaatatatag aaatgttata caagaatcaa aagatcttta tgataaatat ctaagcgtaa
    50161 attctcctaa tagtatagta agtgaaaaag catactcttc tacaagaata accagttctg
    50221 atgaaagtat tatagtagca aaaggctcag ctggtgcaga aaaaataaat tatcaatttg
    50281 cagtttctca aatggctgaa ccagcaaaat ttactattaa attaaattca agtgaaccta
    50341 ttgttcaaca gtttcctcca aatgctagtg gagcgagttc tttaactata ggagatgtaa
    50401 atataccaat atctgaacaa gatactacaa gtactattgt aagtaagata aactcccttt
    50461 gcgcagataa tgatataaag gcttcttata gtgagatgac aggtgaattg attatttcaa
    50521 gaaaacagac tggttcgtca tcagacatta atttaagagt aattggaaat gacaatttag
    50581 ctcagcaaat tgctaatgat aatggtatca catttataaa tgatgctggt ggaaacaaag
    50641 tggcaaatgt atatggaaag aatctagaag ctgatgtaac tgatgaacat ggaagagtaa
    50701 ctcatataag taaagaacaa aattcattta atatagataa tattgactat aatgtaaatt
    50761 caaaaggaac tgcaaagttg acttctgtca ctgatactga agaagctgtt aaaaatatgc
    50821 aagcatttgt ggatgattat aataaactga tggacaaggt ctatggttta gttactacta
    50881 aaaaaccaaa agattatccg cctcttacag atgcccaaaa agaagatatg acaactgaag
    50941 aaatagaaaa atgggaaaag aaagctaaag aaggtatact tagaaatgat gatgagttaa
    51001 gaggttttgt tgaagatatt caatctgcat tttttggaga tggaaaaaat attattgcat
    51061 taagaaaact aggtatcaat gaaagtgaaa attacaataa aaaaggtcaa atatcattta
    51121 atgcagatac tttttcaaag gctcttatag atgatagtga taaggtatac aaaacactag
    51181 caggttattc ttcgaattat gatgataagg gaatgtttga aaagctaaaa gatattgtat
    51241 atgaatattc tggaagttca acttctaaac ttcctaaaaa agcaggtata gaaaaaactg
    51301 cttctgctag tgaaaatgta tattcaaaac aaattgcaga gcaagaaaga aatataagca
    51361 ggttagttga aaaaatgaat gataaagaga aaagacttta tgctaaatat tcagccttag
    51421 aatctttgtt gaatcagtat tcttcccaaa tgaattattt ctcacaagca cagggtaatt
    51481 aattatggat aaattaaaag aaaaattaaa tttatataaa gatatatctt tacaaattat
    51541 aaatttgatt gaaaaggaag aatatataaa gataagtatt aagttaggtg aaagacaaga
    51601 gattataaat agtgtaagtg aaattgatag aaatgatttt atacagcttt ataatagaat
    51661 ggaattaatt gaaattgata gcaggataag agatatttta caaggacaat tattagaagt
    51721 aaaaaaagaa ttacatgagt ataagcttac taaacaagtg aacacaatgt attataactt
    51781 aaatagagaa aaagtaaata tttttaataa aaaagtataa agttatgtac catcttgtcc
    51841 gattatataa atgaagaatt aataaaaaag attaaaagta gaaatgacaa ggatgtcaac
    51901 tatactaagg agggtaaaat aatgagagtt aatacaaatg taagtgcttt gatagcaaat
    51961 aaccaaatgg gaagaaacgt aaatgcacaa agtaagtcta tggagaagct atcttctggt
    52021 gttagaatta agagagctgc tgacgatgct gctgggcttg ctatatctga gaaaatgaga
    52081 gctcaaataa aaggtttaga ccaagcagga agaaacgttc aagatggtat atctgttgta
    52141 caaacagcag aaggtgcatt agaagaaact ggtaacatac tacaaagaat gagaacttta
    52201 tcagtacaat cttcaaatga aactaatact gctgaagaaa gacaaaagat agctgatgag
    52261 ttactacaat taaaagatga agttgaaaga atatcaagct caattgagtt taatggtaag
    52321 aaattacttg atggaagttc tacagaaata agattacaag ttggagcaaa ctttggaaca
    52381 aatgttgcag gaacatctaa caacaacaat gaaataaaag ttgctctagt aaatacttca
    52441 agtataatga gtaaagcagg tataacaagt tctacaatag caagcttaaa tgcagatgga
    52501 acttctggaa caaatgcagc taaacaaatg gtatctagct tagatgtagc tcttaaagaa
    52561 ttaaatacat caagagcaaa attaggggca caacaaaata gactagaatc aactcaaaac
    52621 aacttaaata atactataga aaatgttaca gcagctgaat caagaataag agatacagat
    52681 gttgcttcag aaatggttaa cttatctaaa atgaatatat tagttcaagc atcacagtca
    52741 atgcttgctc aagctaatca acaaccacaa ggagttttac aattattagg ataaaataat
    52801 tttgtatatt ggatatagaa gaaagggtaa ggctttgctt tactcctttc tttttttaca
    52861 aatatacttt tgaaaggaaa atttatatgt tacttagtat agttatgatt gttaaaaatg
    52921 aagaaaaaat acttgaaaaa actttaaaat cattaaccac attaagaaat tctatagaaa
    52981 gtgaacttat aatagtcgat acaggttcta cagatgatac tataaaaata tctaagaagt
    53041 acacggaaaa agtgtatttt cataattgga ataatgattt ttctagtatg agaaatatat
    53101 caatatccta tgctaaaggt gaatggttac ttatattaga tgcagatgaa atactaattg
    53161 attcttcaac tattgtaaaa ttttttaaag atggtcttga taaaaaattt aattctgctt
    53221 caatacaatt aaaaaatctt tactcatatg ataaaaaatt atatggatat tgttcagtgc
    53281 ttagactttt taaaaacgtt gattttagat accatggtaa agtacatgaa caaccaatat
    53341 acaaaaaccc tatatttaat aatgttgctg attttgaaca ttatggatat ttgtttgaag
    53401 atgaagaaat aagattaaat aaggtgaaaa gaaatgaaaa actattattt caagagttaa
    53461 aagagaatga taattctcca tatacaaact atcaattagg gaaaaatttt attatattag
    53521 gaaaatatca ggaggcttta tactatcttg agaaatctta tgaattatat tctaaactag
    53581 aattggcacc tggttacttg ataacaagcc ttgtaaaaac atatttatat ttaggaaaac
    53641 ataagaagtg tgaaagattg tgcttaaagt atattaagaa agacatgaat aatattgata
    53701 tatattatta tattgcacaa gcacaagtag atttaggaaa gtatgaaaat agtatagatt
    53761 cgtataagag atatatttac ttgttagata attatgaaat aagtacacaa gcaaatagtt
    53821 tattttcaga cactgatact gtagggctta gagatgaggc tattattaca ctgataaaga
    53881 tttattataa gcttgaaaaa tatgagtttg taatttcaga gtataataac attgaagatg
    53941 tggaaaagaa aaagaatgtg tactttagct tatttatgtc actatataaa ttgaatagat
    54001 ttgaagatat aaaaaattat tataaagaag tttcaaattc taaaattgaa aaaaatagtt
    54061 tttatagaaa tatagaagaa gttataaata atattaaaga ggatgaaaaa gagtatgtat
    54121 ataaaatcct ttgtgacatt gaaggtaatt atggcatttt aaatcaaata aggataaata
    54181 aatgtgtatc tatagataaa tgtaaagaaa tactcaatga agaaaaggaa ataatttatg
    54241 catatattat aaaaatagct tttgaagaga agattgattt actagatatt ttttatgaaa
    54301 tggaatatgt atggatagag aaatatctaa aatatctttt agcattagaa agaagtttta
    54361 atttagtact atataagtac ataattagtc gtccaaatac atcagatatg gaaaagataa
    54421 gagtatataa agctatatct aaagtagttt tagaaagtat aagcttaagt gatgaaaaat
    54481 ataaggagat tttttatctt tatataatgt attcttataa gtacataaga tacatatata
    54541 gcaatttaag tgattttgaa ttaataaaat ttgtttgtaa tgatttagat aagttcacta
    54601 tagaatttaa gcatctactt gatattaaaa ataaagtaaa taatgctaat gaaaaattag
    54661 agtatataca tagtatgagg aaattactaa atgagtatcc tttttataat aaaataataa
    54721 aaatacttat aggtgaatta gaagaaaata taaaagaaag tgaagaattt aaggatttaa
    54781 aagatagttt tattattagt atagaaaata tgattgaaac tggcaaaata aatgatgcga
    54841 aattattggt agatgactat tcaaaaacat ttgtggatga tataaagatt ttaaatataa
    54901 agggaatttt atacatgttt gaaaataagc ttgataaagc tgattttatg ttaaaaaagg
    54961 cattatcatt agatgtagaa aatgaagata ctatatataa tatagaatac ttaaaaagtt
    55021 taagataagt caaaaatagg agaattgaag aatgaatata ccaatagtgg ttaatgaaaa
    55081 atctaaagac ataataaaag attggcaata tacatgtaca aatgaagata taaaatttac
    55141 aagcataata atattgacat ataatcaaat agaatatact aaattatgta ttgaaagtat
    55201 taggaaattt accccaaaga ataaatatga aataataata attgataata actcttctga
    55261 tggaacagtt gagtggttaa agggacaatc tgatataaaa tctgtgtata ataacaaaaa
    55321 cttaggattt ccaaaaggat gcaatcaagg tattgagtta gctactggtg aaaacatatt
    55381 gttgttaaat aatgatgtaa tagttactcc aaattggctc tataatttga ataaagcttt
    55441 atggtctcaa gataatatag gtgctgtagg acctattacg aatgcatgtt catattatca
    55501 gcaaattgat gttgattata agtcaattga ggacatgttt atatttgcac acaaaataaa
    55561 taaatctaat attaacctat ggaaatatag attgaagtta gttggatttt gcatgttaat
    55621 aaaaaaaact gttctggata atgtaggggt tttagatgaa ttattcacac ctggaaattt
    55681 tgaagatgat gatttatctt ttaggatgat agagtcagga tataagttat tattatgcca
    55741 ggatactttt atacaccatt ttggaagtgt gtcttttaaa gaaaatcgta ataaatatac
    55801 agatataatg tatattaata gaagaaaatt tgaaactaag tggggattta atccagaata
    55861 tagtggaatg ataagaacag atttgatttc taaaatagat gatagtttgg aggcaaatat
    55921 taatgtttta gaaataggat gcggtatagg ggctactctt ttagaaataa aaaatatata
    55981 taaaaatgca tcaatatatg gaatagaaat tagtgagtca gcaggtaaac ttgcaaagta
    56041 tagtgcagat gtacttatag caaatataga aaaagtaaaa ttggattata aaaaagattt
    56101 ttttgattat ataatattgg gagatgtact tgaacatttg attgacccat ggaagatagt
    56161 caatgaattg aaacaatatc taaagaagga tggttatctg gtatctagta taccaaatat
    56221 tatgcatgtt tctgttttaa gaaatcttgt gttaggtaat tttacatatg aagattcagg
    56281 aattttggac aagactcatt taaggttttt tacattaaat gagataacaa aaatgtttaa
    56341 tgatagtaat tacataatta atagtattac agctactagt atgccacttt cggaaattga
    56401 taatagcttc attgatacaa tatgtaagga aagctgtaaa gaaatgagac aacagtacga
    56461 atcttatcaa tatatagtaa aagcctctaa aatcctagat actaaaagat attctagtat
    56521 tagtatgatt aatttaaaat acgctttaat gagaattgat aatgaattag atgtagatag
    56581 taatttaagt tatatatttg ataattataa tataactgaa ccatgttttg ttgatgatat
    56641 tgaatatcta attgatacat ttgtgataaa taaagcaact ctattaaata gattaggagt
    56701 tgaggcattt aacagaggtc taggagattt tagtataaac atgtttatga cagtattaga
    56761 gatggataga gatgatattg actcaatata taatataaca agtgtattat gtgaattaga
    56821 agaatttgat actgcttata atttaattaa aaatagtagc gaaaaagtac agagtgaaag
    56881 tgatatacaa gagatacttt gttttattga ggggaagaaa tatgaataat gataagatat
    56941 gttttataac ctgtgtaaat gatgaaaggc tttatgaaga atgtcttgta tatataaata
    57001 gcctagacat tcctaaaaac tttagtatag aaacagtagc tttaagagga gccgattcta
    57061 ttgcctctgc ttataataaa gctatgagag aaacagattc taagtataag gtatacttac
    57121 atcaagatac ttttataatt aataaaaatt tcatacatga tattttagag atatttaaaa
    57181 aaaatacaga tgtcggaatg attggtgtag ctggagtaaa agatatgcct gtgaatggaa
    57241 tttggtggga ttctgataaa aaagcaggga aagtatatga tagtcataca ggtaaaatgg
    57301 atatttatga atttagtaac tttgaagaat gttttaatga agttatggct atagatggat
    57361 taattatgat tactcaatat gatgtatttt ggagagagga taagtttgat gggtggcatt
    57421 tttatgattt gtcacagagt atggagttta gacaaaataa atacaaagtt attgtaccaa
    57481 aacaaaataa gccttggtgt atgcatgatt ctggagtagt aaatataaat aatggatatg
    57541 atgaatatag gataaaattt gtaaaagagt acataaatct tgatttatat agagaaaatt
    57601 tttatgaatt tgataagaag aaagcaagta ccatacttaa gagtaataag catgtaatgg
    57661 attgtatgaa agaggagctt acagaacaat taagattaaa gaattacaag gatgttttag
    57721 gtattgtttc taaaattgct catttttctg tttttaatca tactggtttg tattcttgtg
    57781 aagaatgtga gagagcattg ctagaatgtg caaataattt accagaaata gaaaatacga
    57841 aattatttac aaaaaaagat ttaaaaagaa gaaaagtatt acatgtttta agtgaaggat
    57901 attcaacagg aggtcataca agactagtta aaaattggat tgaaattgac aaagactctg
    57961 ttcattcagt tgtaactaca tggcaatcag atacactgcc agactggtta ttaaaagcaa
    58021 taaatgatag tggtggcgaa gttatatctt tggatttaat ttcagatgat tatttaaaaa
    58081 gagctgtagc tttgaggaaa atagcttatg actgggctga tatagttatt ttatatgttc
    58141 atatgtggga tacaatacct gttgttgctt ttggagttga tgggaaaata cctattttac
    58201 atgttaatca ctatgatagt actttttggt tagggacaag tattgcagat attgtcattg
    58261 attttagcga aactagtcaa ttaattacat tagataaaag aggcatagat aaatctgaaa
    58321 tattaccaat gccattaaaa gaaaaagaag tattttcaaa atctgaagtt agaaaacgat
    58381 ataatttaga ggatgatgtg acaataatat tgactattgc tagtgaaaga aaatacaata
    58441 gtgtaaatga tattaattac tttaaaattg ctaaaacatt gattgaaaat acagataata
    58501 caattattat tgttgtagga gttgatacag agaatctcta ttggaaaact ttaagtaatg
    58561 ttacagataa tagagttata tgttttggaa caacagcaaa tatagatgat ttttataaga
    58621 tttcggatat atacctagag tctttcatgg taggaagtca tacttcaaaa ttagatgcaa
    58681 ttttatatga tttaattcct gttaagttta aaaatgaagt ttcaccaatt tttacaaata
    58741 tgtggaattt tatagataag tttgattata agaatataaa agaggttata gacatgataa
    58801 gaaggtacaa tactagtgaa tttgacaggg ataagataaa agcaaaacaa tcggaaataa
    58861 aacattttgt tatagataaa cattgtattg aaataaaaaa ctatatagac aaaatatata
    58921 atgaagttaa tcatcataaa gttaagtatg atttatataa aaatgatgaa atcgaggact
    58981 atcagctttt tgtagcatta tatggctata tggtaaaaaa taaaaacatg tcataggtgg
    59041 taaaatgaac aatattcata tcaataaatt tgaaaaaagc tgtgaaaaaa actgggatta
    59101 ttttgtagaa aataaatcta taaatggtac atttctgcaa actagaaatt tccttaatta
    59161 tcatgaggag ggaaaattca ctgaccactc attaataata tgtaaaggtg aagaaatact
    59221 agcagtcgta cctgcatgtg aaacttatga agatggtgaa agaatatttt tttctcattt
    59281 agggagtact tttggtggca ttataataag aagtggtttt aatgatatta ggcatgtaaa
    59341 tgccattaca aataagttag aaatatattt acaggaacaa aatttcgata aagtatattt
    59401 aaaaaataca tcaagaatat tttgtaaaga ggatacttca ttactagaat attttttatt
    59461 taagaataat tacatgtttt atgatgaatt aagtatgttt ttagaattaa gtaagtataa
    59521 agaagatata ttagccaatt ttaaatacag taaaagaaga gattttaaat attctttgaa
    59581 gaataatctt acctttaaaa gactgtatag tgatgaagat atagtttgtt tttttaattt
    59641 actttctaat tgtttagata gtaaaggagc taaagtaata cattcagtag aagaactttt
    59701 ggagtttaaa aataatagat taaaagatat tgtagaattt tatgcagtat atattgatga
    59761 cattatgatt tgtgggagca tggtatttaa ctttcaagaa agagtttatc atactcaata
    59821 tctagcatca aattcgacat ataataaata ttatccaatg atatttttat actataatct
    59881 aataaaagat gctttagaga gaaattttaa gtatctttct tttggaatta gcactctaaa
    59941 tagaggcaaa tacttagatg agggtttagc tacatttaaa gaaggttttg gttcaagtgc
    60001 aatttgtaac agaacatact ataaaaatat aaataagtga gaatcatgtt ttatagagtt
    60061 tttagagtat agtaaatatc tagtactata atattacata aaaataatta gtttataaag
    60121 aaggtttgta tatgaaaaga ttaatgattt taggctcaat taagtatttt gagaatattg
    60181 ttatgtcagc taaaagagag ggaatataca caattgtttg tgataataga attgatactc
    60241 ctgcgaaaaa gatttgtgat gaagcaatag atgttgatgt atttgatttt gataaaataa
    60301 agaatatagc tatagataaa aagatagatg gaattttaac tggatttaca gattctttaa
    60361 tgaagccata tgtttatatt gcaaatgaac taaatttgcc atgtgtgatt tcttgcaatc
    60421 aacttgaatc tgtaactaat aaggctgtta tgaaaaatta ttttaaaaat aatggaatac
    60481 ctacaactga ttattgtatt attgaaagtt tgaaagattt agaaaaaata caaggattaa
    60541 attttccact tgtaataaaa cctgttgatg gttatggttc aagaggtgta tattttgtat
    60601 caaacataga agaattaata gaaaaatttg aatattctaa ggaagcctcg caagatggac
    60661 aagtaataat tgaggaattt tatgaaagtg aggaaataca aggactagct tgggttcata
    60721 atggagagtc acatgtattt tatattggag atagagagct tgtaaatata catacaggaa
    60781 gagctggaaa accagataga ttaatttatc catcaaagta ttgttttcaa tatgaagaag
    60841 aaataaaatc catatatcaa aatataacgc aggcttttgg cataaaaaat ggacctcttt
    60901 atgtacaaat gttagtggga gcatatggaa taaaggtatc tgaagtaatg ccaagattac
    60961 ctggaggttg tgactatatg gctatttctc aaataacaga tttagacata ggaagtttat
    61021 ttgcaaactt tagtgtgaat aatgaagtaa attttgatga aataaagaaa tataatatgc
    61081 atctatcaaa atgtgtatat gcattaccaa tatacataag acctggcaca ataaaaaaga
    61141 taaataatat agataaaata gaaaacttag attatgtcac tcaatttcta ttactagtaa
    61201 aagaaggaga tacaatagga ttatatgaag atgagagaca ggattgtgga agagtttatg
    61261 gagtagcaaa taatgtattt gatgcaaata taaagaagaa atgtattcat gaaatgatag
    61321 aaatacttga tgaaaataat gaaaatatga tagaaaattt ttaaataagg gagtatgata
    61381 gatatgaagc taataactca tgaacaaatt atgtcattaa acatttctca gaaacaagga
    61441 tacgagtgga taaaagaatc ctttttaaca aaaaaagatt gtcaattacc accaaaaata
    61501 agcatggtag aagaagggca tgtattttac aatgtaatgc cgtccctaaa tcataatcat
    61561 aagattggtg gaataaaaat ggttacaaga ttgccaggga gaaatccaag tttaaattca
    61621 gagatatttc tatataatat ggaagatgct aaattaaagg ctattttaga tggcaattat
    61681 attacagctt ttaggactgc tgctgttgct gccttggcta tcgatacatt tgctaaaaaa
    61741 gattttaata ctattgctat tatgggttta ggtgtaactg cttctgctat aatgaatatg
    61801 ctttcatata ctcttaaaga taaaaatata aatataaagt tacttaaata taaagaccaa
    61861 gcagagtcgt ttgtaaaaaa gtatgaaaaa aatgaacaat ttagttttga aatatgtgac
    61921 acacatgaag aacttattgt agattcagat gtagttattt ctactgtgac atatacagat
    61981 gaatatttat cagaatttaa atggtttaaa aaaggtgctt tattattacc tgtacatgtt
    62041 atgggatttg gaaatgttga ttatctattt gataaagtat atttggatga ttataatcat
    62101 ttgaaacatc tagaaaatat agatagatat aaatattctt gtgaatttta tgatgtttta
    62161 agtaaacaag ctaaaggaag agaaaatgat gatgagagaa taatatctta caatataggt
    62221 ttgtcaatac atgatatatt atttgcaaat aaaatttacg aattgtgtga aacactaagc
    62281 tgtgaagaaa tagaattaaa agaaccaaaa gaaaaatatt ggatatagtt tatttcattc
    62341 atatttacaa tagtttattg taaatatgaa tatgaagtaa tatattgtta gaaaattagc
    62401 atgaatagga tagataaatt tatggataaa tataatttaa ttaggttcgt atctattgga
    62461 aacgaatata taggaaattt agttgcatta gaatcaaata aagcaatacc ttttgatata
    62521 aagagagttt attacatata tgatgtacca aacaatgtaa agagaggatt tcatgcacac
    62581 aaaaacttgg aacaagtact agtttgccta aatggaagtg tgaagataaa atgttatgat
    62641 ggtgtaaaag agactatatt taatttacat aaaaatgatg ttggattata tataggaacg
    62701 ggagtgtggc atgagatgtt tgattttgat gaaaaaactg ttttaatttc tatagcatct
    62761 gaacattata atgaagaaga ttatattaga gactataaag aatttttaaa gtatttaaat
    62821 aatgtgtagt tattatacaa ttagtgatgt attttcttaa ttaaataaat ttaagatagc
    62881 cacatagaga atagatagat taggatgtga atttattgtt ggttaaattt aataatttat
    62941 taaaacagtt ccaaactcat aaagaagaat atgaacagaa gattaaggat gtaatggaaa
    63001 gtggatatta tatccttgga aatgaagtaa agcattttga agaagaattt gctaactatg
    63061 taggtagcaa atattgtgtt ggtttagcca gtggtttaga tgcactgata attgctttta
    63121 gagcattagg aataagcaaa ggtgatgaag taatcgtgca atcaaataca tatattgcta
    63181 gtgttatggg aatcagtata aatggagcaa caccagtatt tgtagaacca aatgaatatt
    63241 ataatataga ttcatctaag atagaagaaa agataaatag caaaacaaaa gctatattag
    63301 tagtgcattt atatggtcaa gcaagtaata tgaaagatat acaagaatta tgtgataagt
    63361 ataacttgta tttaattgaa gattgtgctc aatctcatgg agctaaatat gatggaaaaa
    63421 tgacaggaac atttggtgat ataggttgtt ttagttttta tccaagtaaa aatttgggtg
    63481 catttggaga tgcaggagct atagtaacta gtaatgagga aatagctgat aaagtgaaaa
    63541 ttcttagaaa ctatggctca gaaaagcgat attataataa atttgtcgga tataattcaa
    63601 gattggacga aattcaggca ggtttattaa gagtaaaact taaatatcta aatgaaattg
    63661 aaattgaaaa atcaaaaatc gtagatagat atcttaatga aattaataat cccaaagtca
    63721 tacttccaaa gatagaaaat aattgtactc atgtatggca tttatttgtt ttacaagttg
    63781 aaaaacgaga tacatttatg gaatacttaa agaaaagtgg tatagagtct ataatacatt
    63841 atccgatacc accacatcta tctgaagcat atgcttattt gggatataaa aaggggagtt
    63901 ttaatatagc tgagaactat tcagataaag ttgttagtat acctttattt attggtatgg
    63961 aagatgaaga agttagttat ataattaaaa ttataaatga atattaaact actcataata
    64021 atatcaaaaa taaa
//
LOCUS       DAMKMI010000005        62237 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMKMI010000005 DAMKMI010000000
VERSION     DAMKMI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.005"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(188..1789)
                     /locus_tag="PC429_000321"
     CDS             complement(188..1789)
                     /locus_tag="PC429_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3845845.1"
                     /translation="MIYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTAINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIARQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            complement(1900..2577)
                     /locus_tag="PC429_000322"
     CDS             complement(1900..2577)
                     /locus_tag="PC429_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845846.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            complement(2590..3285)
                     /locus_tag="PC429_000323"
     CDS             complement(2590..3285)
                     /locus_tag="PC429_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF3845847.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            complement(3337..4536)
                     /locus_tag="PC429_000324"
     CDS             complement(3337..4536)
                     /locus_tag="PC429_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HDF3845848.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARNLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            complement(4797..6842)
                     /locus_tag="PC429_000325"
     CDS             complement(4797..6842)
                     /locus_tag="PC429_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3845849.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKYFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            complement(7253..8062)
                     /locus_tag="PC429_000326"
     CDS             complement(7253..8062)
                     /locus_tag="PC429_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3845850.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQEFLQFLEE"
     gene            complement(8146..8679)
                     /locus_tag="PC429_000327"
     CDS             complement(8146..8679)
                     /locus_tag="PC429_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF3845851.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            complement(8829..10178)
                     /locus_tag="PC429_000328"
     CDS             complement(8829..10178)
                     /locus_tag="PC429_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF3845852.1"
                     /translation="MELKNFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            complement(10190..11572)
                     /locus_tag="PC429_000329"
     CDS             complement(10190..11572)
                     /locus_tag="PC429_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF3845853.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRVRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            11816..13030
                     /locus_tag="PC429_000330"
     CDS             11816..13030
                     /locus_tag="PC429_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF3845854.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            13207..15120
                     /locus_tag="PC429_000331"
     CDS             13207..15120
                     /locus_tag="PC429_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3845855.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYKNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            15391..16365
                     /locus_tag="PC429_000332"
     CDS             15391..16365
                     /locus_tag="PC429_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF3845856.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            16386..16661
                     /locus_tag="PC429_000333"
     CDS             16386..16661
                     /locus_tag="PC429_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF3845857.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            16674..17837
                     /locus_tag="PC429_000334"
     CDS             16674..17837
                     /locus_tag="PC429_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF3845858.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGSAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKTESLGHREFGIYRISSTF"
     gene            18174..20453
                     /locus_tag="PC429_000335"
     CDS             18174..20453
                     /locus_tag="PC429_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3845859.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQSISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVVAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVMNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(20548..21888)
                     /locus_tag="PC429_000336"
     CDS             complement(20548..21888)
                     /locus_tag="PC429_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3845860.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(21900..22682)
                     /locus_tag="PC429_000337"
     CDS             complement(21900..22682)
                     /locus_tag="PC429_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3845861.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            complement(22697..23719)
                     /locus_tag="PC429_000338"
     CDS             complement(22697..23719)
                     /locus_tag="PC429_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF3845862.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            complement(23725..24738)
                     /locus_tag="PC429_000339"
     CDS             complement(23725..24738)
                     /locus_tag="PC429_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF3845863.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTKFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            complement(24866..25804)
                     /locus_tag="PC429_000340"
     CDS             complement(24866..25804)
                     /locus_tag="PC429_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3845864.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEDKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            26074..26538
                     /locus_tag="PC429_000341"
     CDS             26074..26538
                     /locus_tag="PC429_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845865.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            complement(26650..26967)
                     /locus_tag="PC429_000342"
     CDS             complement(26650..26967)
                     /locus_tag="PC429_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3845866.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(26980..27711)
                     /gene="chbG"
                     /locus_tag="PC429_000343"
     CDS             complement(26980..27711)
                     /gene="chbG"
                     /locus_tag="PC429_000343"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF3845867.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            complement(27737..29047)
                     /locus_tag="PC429_000344"
     CDS             complement(27737..29047)
                     /locus_tag="PC429_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF3845868.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            complement(29144..30457)
                     /locus_tag="PC429_000345"
     CDS             complement(29144..30457)
                     /locus_tag="PC429_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3845869.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            complement(30479..30790)
                     /locus_tag="PC429_000346"
     CDS             complement(30479..30790)
                     /locus_tag="PC429_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3845870.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            complement(30990..31694)
                     /locus_tag="PC429_000347"
     CDS             complement(30990..31694)
                     /locus_tag="PC429_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3845871.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCEYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            complement(31785..33146)
                     /locus_tag="PC429_000348"
     CDS             complement(31785..33146)
                     /locus_tag="PC429_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3845872.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            33645..34799
                     /locus_tag="PC429_000349"
     CDS             33645..34799
                     /locus_tag="PC429_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3845873.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            complement(34881..36599)
                     /locus_tag="PC429_000350"
     CDS             complement(34881..36599)
                     /locus_tag="PC429_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF3845874.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(36604..37593)
                     /locus_tag="PC429_000351"
     CDS             complement(36604..37593)
                     /locus_tag="PC429_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3845875.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            complement(37689..38738)
                     /locus_tag="PC429_000352"
     CDS             complement(37689..38738)
                     /locus_tag="PC429_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3845876.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            complement(39015..39683)
                     /locus_tag="PC429_000353"
     CDS             complement(39015..39683)
                     /locus_tag="PC429_000353"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF3845877.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            complement(39689..41062)
                     /locus_tag="PC429_000354"
     CDS             complement(39689..41062)
                     /locus_tag="PC429_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF3845878.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            complement(41059..42837)
                     /locus_tag="PC429_000355"
     CDS             complement(41059..42837)
                     /locus_tag="PC429_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF3845879.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            complement(42954..43277)
                     /locus_tag="PC429_000356"
     CDS             complement(42954..43277)
                     /locus_tag="PC429_000356"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF3845880.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            complement(43264..44241)
                     /locus_tag="PC429_000357"
     CDS             complement(43264..44241)
                     /locus_tag="PC429_000357"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF3845881.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            complement(44256..44819)
                     /locus_tag="PC429_000358"
     CDS             complement(44256..44819)
                     /locus_tag="PC429_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF3845882.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            complement(44871..45362)
                     /locus_tag="PC429_000359"
     CDS             complement(44871..45362)
                     /locus_tag="PC429_000359"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF3845883.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            complement(45365..47290)
                     /locus_tag="PC429_000360"
     CDS             complement(45365..47290)
                     /locus_tag="PC429_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF3845884.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            complement(47292..47603)
                     /locus_tag="PC429_000361"
     CDS             complement(47292..47603)
                     /locus_tag="PC429_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845885.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            complement(48233..48508)
                     /locus_tag="PC429_000362"
     CDS             complement(48233..48508)
                     /locus_tag="PC429_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF3845886.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            complement(48623..50032)
                     /locus_tag="PC429_000363"
     CDS             complement(48623..50032)
                     /locus_tag="PC429_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF3845887.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            complement(50403..51065)
                     /locus_tag="PC429_000364"
     CDS             complement(50403..51065)
                     /locus_tag="PC429_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF3845888.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            complement(51319..53538)
                     /locus_tag="PC429_000365"
     CDS             complement(51319..53538)
                     /locus_tag="PC429_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3845889.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFFGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            complement(53814..56468)
                     /gene="adhE"
                     /locus_tag="PC429_000366"
                     /gene_synonym="adhC"
     CDS             complement(53814..56468)
                     /gene="adhE"
                     /locus_tag="PC429_000366"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF3845890.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            56972..57172
                     /locus_tag="PC429_000367"
     CDS             56972..57172
                     /locus_tag="PC429_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845891.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            complement(57379..57969)
                     /locus_tag="PC429_000368"
     CDS             complement(57379..57969)
                     /locus_tag="PC429_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF3845892.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(57972..58847)
                     /gene="dpsA"
                     /locus_tag="PC429_000369"
     CDS             complement(57972..58847)
                     /gene="dpsA"
                     /locus_tag="PC429_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF3845893.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            complement(58923..60314)
                     /locus_tag="PC429_000370"
     CDS             complement(58923..60314)
                     /locus_tag="PC429_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF3845894.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            complement(60318..61454)
                     /locus_tag="PC429_000371"
     CDS             complement(60318..61454)
                     /locus_tag="PC429_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF3845895.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            complement(61486..62031)
                     /locus_tag="PC429_000372"
     CDS             complement(61486..62031)
                     /locus_tag="PC429_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF3845896.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
BASE COUNT        20724 a        10810 c         7446 g        23257 t
ORIGIN      
        1 attaaaaata ttaatttttt cactataaag tataaatatt ttttgtataa atattatctg
       61 attattaaaa taaatattaa tactataaat aaaaacgaag catgattttt atttaaataa
      121 atctcaaact tcgtctttac ttacttagat atttatttac tttctaaatc ctattatttt
      181 taaaacctta tctttttaat acttcaccac atttattctc agtaatctct ccattttcca
      241 acgcaatttt tccatttaca attacatatt caacaccatt ggctagtaat tgtggctttg
      301 aaaaatcaat atcttctgac ctagaataac tatagttatc catgtcaata actgctatat
      361 cagctatcaa cccttcttta agaatacctc tattttttat tttaaattgc ctagctggta
      421 atgaagtaac tcttcttaca atctcttcca atctaacacc agactttctg tacatattga
      481 agaaaattgg aaaagctcct cttctttgta gttcaaacca tgacatatcc ccacccacac
      541 taaacaaaca atctgaacca accataacaa atggattatc aattatttta tcatcatgta
      601 gtttatctgg gaaatcagct ctatacattc ctcctaaaca aaagataagt tcttcgtctc
      661 catctaaaag taaatcgagt aataacttgt cttcatctat tcctttttgt gtagctattt
      721 cacgtattga tttcttttcc atattaatat ctttagtgtt tagaagtatt atactccccc
      781 tatctccaag tataaatgct tcttttaata taagtgttct tccctcttca tttctaagag
      841 catccattac cccatttata cctaattcaa atagactaga tgatattgcc ataataaatt
      901 gaattactct ttttttcttc atacaatgcc cagaacttct tggtactgtg tcaaccatta
      961 tttcaacacc attataccta gcctttaata tctcatcaaa tgcttctatt tcaactggac
     1021 ttaagtgaga tacttgaacc ctagcaccac ttttttgccc aacctctatg gcttctttta
     1081 ctgcattata tcttcctatg taatctctaa tatgagatgt atatatacca tcatactctt
     1141 taatctgcct tgcaatatca acaagttcat caatatcagc atacttactt ggtatatatg
     1201 ccaatccagt agatattcca aaagcaccac ttttcattcc atcatttatt atatctgtaa
     1261 tttcattttt ttccttttct gtaggaggtc tatccttaga acctcccata acactccatc
     1321 ttatagtccc atgccccaac aagaatccca tattgacaga aataccactt ttagatataa
     1381 ggtcacaata ttctgtaaaa ttattccaac atttattttt atctataaga tacttttttt
     1441 cttcttcaag taatagacca tttttataca tatattcata cacattttca gaagaatatg
     1501 gtgttataga atgtccacaa ttaccattta tagctgttgt aactccttgt tttagaaact
     1561 tttcaaattt accatctagt attgctacta tttcttcatg aacatgaggg tcaataaatc
     1621 caggtgttat aacttttcca caacaatcaa tctctttatt tgtattgcta tttatcttag
     1681 aatctatttt tgctattaag ttgtctttaa ttgctatatc tccataaaaa ccaggattac
     1741 ctgtaccatc tattataaat ccatttttta agattaaatc atatatcaaa atattccctc
     1801 cttaaaaacc aatttttaaa taatcttaat gtaattttta tatttgaaaa tcaaattatt
     1861 aaatttcata tttgaaaatt cactattatt tcattgtaat tagtcactaa gcttatcttt
     1921 atacatagct cttgcaatta atatagtacc aaatacacat ataagaacta ctagagactg
     1981 tggtattata ccttgctttg ataatataat agtcaataca gatattacaa ttgctactag
     2041 acctaatttt ttatcttgta aaaatacttg tccaaaaact gacccaaata gagctggtaa
     2101 aattaagttt aatttttcta ctacttcagc tggaatttta gatagtacgc ttcctcctaa
     2161 aattacccct atagttagta tacttatatt cactaaaata gatacagcta ttcctattgt
     2221 agatattatc gagccttctt ctgtacctga ttcaacttct gctgcctttt gagctgctat
     2281 tgaacatggt agcctcatat tagatatatt accagaaaga aaactcatat aagttcctgg
     2341 tatacctaag attggtgaat aagaaactgg ttctattatg taatttggag cactcatact
     2401 gacaattgcc attgtacttg ctaataatgc tgatattgga ggcataatac caaatccaaa
     2461 gctcaaaatt attccaggaa ctaatgctgc tataataccc aaagatagtg ttagtcttcc
     2521 ataccttatc ataaaaggta aatattcttc attgtatata gattttactt tactcataaa
     2581 actcccccct tatattactg aagctattat cattccgcta aacattgata ttgtaagccc
     2641 ccactctctt aaccatttta tatttttctt cttttcaagc atacacagta ttaccattat
     2701 agccattcca cttatacatg caattgttcc attatcaaaa cgcaacaccc tatcagcatt
     2761 tagatatgca aatgaaccca acattgctcc tagagatatt attggtataa atgatttctt
     2821 gccactagat ataacattgg ttatcttatc tagtttatgt ccaaatagta atgtgaatat
     2881 tatccatcct aaagaaccta atatcatagt ccaaactgca tttgtaaatg caagttttgt
     2941 catttcagga gaacctaaag ttatacctaa tgcatctgtt ccaaatccgg ctgacattga
     3001 ttcaaaaatt acagaaccta taaatgataa tctcatccat gctataggcc cacccatagt
     3061 tactataagc gataccattc cagcaaatat agtcattgaa ggcccaatag ctgaaatagc
     3121 actactctta acagctgact ttatttgttt atctgttatt cccattgttt taccaacttt
     3181 gcaagatttt atagcaaata taagggattg taccactact attgatactg ctactgtact
     3241 cgctatccac aaaagtggat gattagctaa ttttaaataa tccatttata aaacctccct
     3301 ataaatcctc aaattttatt aatatttcta atatatctat ttccaaacct gttttagtac
     3361 tctaagtata tttccaccta gtaccttctc tatttcctca tcactatatc catgctttac
     3421 taaatatcta acaatgtttt ttgaagtttc tgttgggttc tcaagacctt ttacatattc
     3481 tactttggga tgttcttttg ttcctgttat tttttttata tcaaatttag cagctagtac
     3541 tgagtgtaat tttacatggt cgccatatac tgtatctggt ccaaaagcta catggtctat
     3601 tcctactaaa tttttaatat actcaaaatg ttccataaat gactctattg aatgttcttt
     3661 attcttctct gttattgtag tatgaggtgc tgcttctaca cctataaccc ctcctttttt
     3721 agcacatgct attaatactt catctggagt tagtctcttt gtgttccata aatttcttgc
     3781 cccaacatgg cttaatatta ttgggtcctt gctatattct attacatcaa gtgtagtttg
     3841 aactccaacg tgagagcaat ctattgccat tccaatttta ttcattcttt caacagcttt
     3901 tttcccaaaa tttgtaagac ctccatcttt ctcttctttt agtcctgaac ctaatgcatt
     3961 ggattcgctg tatgttatac ccattaacct tataccaaat ccatacagta tatctattct
     4021 atcaagttca ttttctattg gtgctgcacc ttctaatgtt ggtatcaatg ctactttacc
     4081 ttcactttta gctctgtata tatcttctac tttttcacac tttatgacaa aatcttgatg
     4141 agctaaatca cataatctca tgcctaaatc tattaatata tcatcccatt tccaccctga
     4201 atttgatgtt atcgtacata caccatccat taaattatca aatatagcat cataatatcc
     4261 tcttgataat gcttcataag cacatctttg tttaccttcc ctattatatt caaatatatc
     4321 tctttctagg tgttctggaa ataatactgg atgttcatgt aatgatataa atattttttc
     4381 atccactata cttttaacct tagcttcttg aatatcatct aattcaataa ggtattcttt
     4441 aactctttta tctcctttac acatctcaat agatgaaata tcttttcctt tttctaaata
     4501 actataggct tcatatcctt tgtaagctga cttcataaca acatcctcca agttctacaa
     4561 ttttttatat ttttcctatg tagcaattga tgtgccaaaa tataaatttt aattgtataa
     4621 taaatcttgc aaaaaataaa aacagtagta aggttgatat ttactactgt tttattaaaa
     4681 ataatcttct tagattattt attcaatatt tttgaattat cttaatctta aagataattt
     4741 tatttatttc tatccattat agggattttt ttagggtgtt ttgccttttt agtcccctat
     4801 atcactccct taatttttac atatgatata aagttctttc cttcatcagt tataaaacta
     4861 ccttgttttt ttactccaga taaaagtaaa ccatggctct ttaacttatc tattcttttt
     4921 ctaatttggt ctacacttaa aactatatct ttttctttta aaatctcttg cagtttattt
     4981 cttcctaata atatattttt attattccaa gtttctatac tggtcaatat acatatactt
     5041 tcctcaaaga aattagattg tttgaaatct aagataattg aatcgaaatt ttcattaact
     5101 aaagtattgt ttttttcaaa tctaaattgt tctggcaaat gctcgtaatc tactctatct
     5161 tcctcaacta tagtatcaat ataataaatt aaatttttaa gctctcttac atttcctggc
     5221 cattcataca atgttaaagc ttgcattgat tcctctgtaa aatatttgtt gctatttatt
     5281 tcatctaaaa aatacttact tataagaggt atatcttcct tacgttctct aagtctaggt
     5341 atctctatat gtaatacatt aatcctataa tacaaatctt ctctaaaaga accttcttta
     5401 atttttttct ttaaatcttt atttgttgca gctataattc taacatctat tggtataatt
     5461 ttacttccgc ccattctcat aacttctttt tcctgtaata ctcttagcaa tctctgctgt
     5521 acatctaaag atatatcacc tatctcatct aaaaaaattg ttcctgtatg tgctctttca
     5581 aatataccca tttttccacc ttttattgct cctgtaaaac ttccttcatc atagccaaaa
     5641 agttcacttt caataagtgt atctgacaaa gaagatagat taactgcaac aaatggcttg
     5701 ttttttctta gagattcatt atgtattgcc tgggcaaata tttcttttcc tgttccattt
     5761 tctccaagta tcaacacaga aaagtctgta gttgctattt ttcttgcaat attaattttt
     5821 tcttttatta ttttactctc acctacaaca ctctcaaatg tatactttga tacaaaacct
     5881 tttgactgaa gtttattttg aatcttgtcc tcaagcactt gaattgcact tatattttta
     5941 atgcttataa tactctttat tcgttcatta ttttcataaa catttacttt attaattatc
     6001 agttttttgt tatttaaatt tactacttca tcattaactt catcttcttg aaaaaatatt
     6061 tttttgacaa ccttatcact aaataagtcc ataaagttat tagatataat ttcattttgg
     6121 tctattccaa ctagattaga aaatactttg ttgcaatata taaattttcc taatttatca
     6181 acagaagcaa taccttcatc tataatctcc aaaaaactct tcatggtttt attcatagtt
     6241 gtataatacc tatatccact tactgtatct tcattgtatt tttccttaat aatatgtaat
     6301 ttatctatag atatatttag ttttgtaaag atttctataa ctgtatttat atcaattacc
     6361 ttatcaccta tgtcaattat ttgctttatg ttttgtggta tgctgtttct gcttcccgta
     6421 ataattccaa tctcacattt agacttatca caacctggat aatatggtat tagattaata
     6481 tgaattaatc ctagttttct aattatcttg gcagtttcat ctgctgactc tttataagca
     6541 tctataacca taacatcaga atcattttct atacttatta tctgatttat attttcaata
     6601 tttatagtac gatgtactat tacaataggt atatttttat caatattatt atgtatatat
     6661 tcctttatat cattgtctga acatactaca agctcatagc ttgacagttc aatatttatc
     6721 ctatttacat ataccaagtt atctatatta caaaattctc caaatatatt ttccaattgt
     6781 tttgatatat ctatatttat tttgtcatca gttgatacaa ataaaatatt atgttttttc
     6841 atagtcccac ctcctgttta acgagtatag aaagtaataa tttggtctat tgtagcactt
     6901 tttaaaaaag ttttaaatct taaaaatcaa attttgtacc atttactatt attttccata
     6961 ataaaataat gtaagctatc ttttttcaca tataatttaa tttctaaaac ggttagtttg
     7021 acattattgg aatataaaca aaaatttaat ataaattcgt taatatatta atacattttt
     7081 aagaatgaca aggaataaat tataacaaat tttaggaata tataataata gggtcttaga
     7141 acttagttac aagttctaaa accctatttg tttagtgtaa aaaacatcat aaaatttagc
     7201 ttaaatcttt tcacataata ttactctttt tctaagtgat aataaaatat atctattcct
     7261 ctaaaaactg taaaaattcc tgattaaata gtttaagctc atcatagaat atagcatgac
     7321 cactatactc aaaagtatat aaaattgaat cttctatttt ttcattcata aaaactgcaa
     7381 attcatacgg acatatttca tctaatttcc catgaaatat accagtaggc actcttacac
     7441 attttaaatc attaaataat tcttcatctc ttagagatac agcagttcca atagtgccaa
     7501 tacctgatgc actaaaacca atatcattaa accatcttct aaaactttct ggtggatttg
     7561 atgcaaatac tttttcgcca aaatcggtta ccatttctgg tctatcagta catgcttgtg
     7621 ctatcaattt atttacatct tcacgtgtca taccatatgg aaattctgga ggtctttgta
     7681 caaatgatgg tgcagcagct gcagctaata ccaattttga cactccatat ccattaaata
     7741 aactcatata cctaagtacg attgctcctc ccattgaaaa accaacaagg gtaaagtctt
     7801 ttaatccaat agcatgtacc accttataaa tatcgtctgc taattggcta taagtatatc
     7861 ctcctgatgt tgcatcagat ttaccaaaac ctcttaaatc aattgaaact gttctatatc
     7921 cgagttttgg caatatattt gtttgatatt caaatatctt atgtcctaag ggccaaccat
     7981 gtataaaaag tacagttttt ttagcactcg gattcaaatc atatactgct atgtttatat
     8041 cattcacata tacataatac atatcaacag caactccctt caagaaaatg tatgatggtt
     8101 attttatata tatgaataaa atgatgaata actaaatttt caaatttatt tacaatactt
     8161 ctctattgcc ttttcaaata aacttacatg aagtttttca tctaggatta ttctatttaa
     8221 tatagctttt atataagggt cttcaataag acttatatct ttataataat tattaatagc
     8281 cacatgctcc aaatgcaaat ctagttttaa tctgttacaa atagaatttc cataacatac
     8341 aaaaccacca ttccaatatg caccacaaga acttgtagaa cctctatata caggatttcc
     8401 acccaataat cttatagtct ttgctaatat ctccatatgt aacatttctg ttatagatat
     8461 atttatccac atttcactta actctctatg cgtttcatct aaatcaaaat tatggtatag
     8521 atattgattt acactcgtaa attcacttga tactccggca tagtcatcca tcaataattc
     8581 tgcatagtac ttatttggac ctaaaacttt tatctctgga tatggctcat cacttgaata
     8641 tccatttcgc ttgtgcatgt cataatgtgc atcactcata atatctcctc ctttaaattt
     8701 aatgtatgat attaattgta tgcatcacaa ctttttcata taacttagat aataaaatct
     8761 acttaaataa taaaaagcca tattaattaa aattaacatg accttttatc cacaaaatta
     8821 ctttttaatt attcatcttt tccagctact gatagctctt taataacaac tgaaggtgaa
     8881 cctatactac tcattggaaa ttctaaatct tctcctatag cttcaatatc tttcaacagg
     8941 tcaaaatagt tccctgctac tgtaatttgc tccactggaa atactttttt tccttctttg
     9001 atataaaagc ctttggcagc taaagaaaag tctcctgtta ctgaatttgc acctgaatga
     9061 agtcctgcaa agctagtaac catcaatcct tcatctatat cctttataat ttcatctaaa
     9121 gatttatctc ctttctctat ataaaaatta gttggatata tgcttatagg tgatgaatat
     9181 gaagatttta ttccatttcc tgtagtcttt acacctgcct tattagccgt ttttaagtta
     9241 tgaagtaaag ttataagctt tccatttaaa acaacttctt ttttaaatgt agctactcct
     9301 tcatcatcaa aaggagtaga tgccatacca ttttctaaaa gtgggtcatc aactattgta
     9361 actataggag atgctatcat gtctccttct ctatttttca ataaagataa tcctttttga
     9421 gctgaatctg cattaaatat cccagaaaat gtgctaagca aagataccat agcttcatta
     9481 agcaatatag ttttatattt acctgatgga atactcttac cacctacttt agataaagca
     9541 ttatcaacac caagttttgc tatctcacaa ggttttattt cttcaataga atttgctata
     9601 ttatatccta ttccatcata tttttgtcca ttgtcttcta tgataggaac tacaaaccct
     9661 ataagcatat tagttttatt gcttaaatta agacctttag tattagatat accattactt
     9721 gatacagaat aagatatagt acatttagat aaatttacca ccttatctga ataagcttta
     9781 gtctctcttt ccatttctaa agctaaatca atcaacttat ctgcctctaa attttctagc
     9841 tctttagaat aagtctttac atcattatag tgcttatctc cttcatatat aaattgtaca
     9901 tcttcatttt caatagtgtt tactccatct tttacatttt ttatcaacat atcaacagct
     9961 ttatcatcta aaatttctgt ataagaataa cctatctttc catttacctt acctctaaaa
    10021 gataaaccaa aagatttatc aagattgtat ttctcaactt ctccttcata tatatttata
    10081 cttaaatttt ctcctgtata ataatatatt tcacactctt caaaaccttc acttagagcc
    10141 tttttaaata atatatcttt aaaattttta agttccacaa ttatccctcc tatcttccac
    10201 ctacagtaat ttcttttacc cttatcatag gttggcctac attagttggt atacttcctg
    10261 atgatgagcc acacatcccc tgagcttgtt ttacgttatt cccaaccata tctatattca
    10321 taagtatatc gctacctttt ccaattaaac tagcacctct aactggctct tgtatttctc
    10381 cattttttat taagtaacct tctgaaactg caaaattaaa ctctcctgtt acaggattta
    10441 cagaaccacc acccattttt ttagcataaa gtccatctgg tattgacttt ataatatctt
    10501 ctggtttatc ctctccagca gcaatataag tgttagtcat ccttgaagtt ggtgcaaatt
    10561 tgtagttctg tctacgagaa cttcctgtag gattcattcc cattcgtcta ccattaagtt
    10621 tatctatcat ataagacttc aaaattccat tttcaatcag tatattcttt tgagttggat
    10681 ttccttcatc atctatattc atagaacccc agtagtttgg tattgtacca tcatctatag
    10741 ctgtaatctt tgtggaagca atctgttgac ctaatttatc tgcaaataca gagttcccct
    10801 ttgcaactga tgtagcttcc aaagaatgtc cacaagcttc atgaaatatt actccaccaa
    10861 atccattatc tattgctacc atcatattac ctgcaggaca atttttagca tgcaacatag
    10921 tatgcgctac tctagctgat tcttttgcat aatattctgg gtctatttct tcaaatattt
    10981 caaacccttt acaaccccca ggaccttcaa atccagtttg attttcatta cccttagatg
    11041 caattgaact tatccccagt ctggttctta ctcttctatc ctcaacatat ataccttcag
    11101 tgtttgcaat taatattttt tggtctttat ccaaatagct aacacttact tgagatatat
    11161 cattactaaa ttcttttgca ctcttatatg caaccttcat aacacttact ttatctctat
    11221 ttcctattga atttggataa atttttatat tatgtatgtt atttatttta atactgtcat
    11281 ttaatattac actttttcca tcttccaatt tacctaaagc aacagcagct ttgtatgcta
    11341 catccaacag actatttaat gaattgtcac ttgtataagc atatatactt ttcaatcctt
    11401 taaatattct tattcctata ccataatttc ttccacctat tgcattttct actttattat
    11461 ctattaaact aatagaatta tttatagtgt cttcttcaaa aatctcagca aaatctcctc
    11521 cagtaattag acacttttct aataccttag aagctatatt ttttgaaagc ataaatttac
    11581 ctcctacttt aaatttttaa ttaactttta ttcaatcttt ttacttgtta acaaacatta
    11641 tatactatat tacttaaaat aaggtaatta tgaatatatt atacatatgt tttatactaa
    11701 aatgttatca cattttatct atacataccc atataactag ataattaaaa tgtataatat
    11761 tatttgctat agaaaataat acactcatac ataataaatt ttaggagtga agtatatgaa
    11821 tcaagaatat gtaaaaggaa atcctatttt tactaatatt aataaagttc caagacaatt
    11881 tccatacctt actgatgata ttgatactga tgttataatt gttggtggtg gcgtaactgg
    11941 atgtatttgt gcttactatc tagctaaaaa caatataaag tctgtcattc ttgaaaaagg
    12001 aaggattgca catggaagca ctagcgtgac aacttctcta ttacagtatg aattagatga
    12061 caatttgata gatttaacag aggttatgac tctaaaagat gctctaaaag catataacct
    12121 ttgtacttca gcacttgaag aattagatac atttatagag ctatatggaa ataagtgtga
    12181 ttatgccaaa agagatactc tactttatac tgccaacaaa cttgaagtta aggctatcaa
    12241 agaagaatat aacttaagaa aagaaaatgg atttgatgtt gaatatattg atgaatccac
    12301 aaaccctttt agctttgatt taaaatcagg attgatagca aagaatggag gacgagaatt
    12361 agacccatat aaatatagtc accatttgat tgatgtaagt ttaaaaaatg gtcttcaagt
    12421 atatgaaaat acagaagtaa aaaaagtaga tttatcaaat gataaggtta ctgctgaagt
    12481 atcgtatgga tacaaagttc atggtaaaaa attaattgta gctacagggt acaatacaag
    12541 tttatttaca aaaagaaatt ttgcaactaa gtcaaatact tttaatatag caacgaaacc
    12601 acttaaaaac atagcttctt ggaaaaacaa cattttaatt agagataact gtgacccata
    12661 taattatcta agaactacta aagacaatcg attaattata ggtggtgaag atgtaagttt
    12721 tgatgatata gaaaatgaaa ctttagccaa tgaaaagtac gatattttag aacaaagatt
    12781 gaaaagtatg tttaaggata taaaagacat tgaagttgaa tataaatatt gtggttgttt
    12841 tgcttcaact ttagataatt taggttttat tggtccagat aataaaaata acaatctatg
    12901 gtattgctta ggttatggag caaatggtat actctttgca atattaggag gtataatgtt
    12961 aagtgaatta tatcttggca aacaaaataa aaatatgaaa ttatttaaag tagatagatt
    13021 tgataaatag atattatata ttaaaagact gtaaaaagat gaaactttat aattctaaaa
    13081 gttaactttg atacagtctt ttataatgtt ccttttaaaa atactttatt tataattaaa
    13141 tataaagtaa aatgttaaaa tataactgat aaaatttagt cataagataa tatttaagtt
    13201 tatttgatga aatatggagg aattattatg aaggaaatac tcgttattgc tcccactaaa
    13261 ggaacctatg aaaaatcaat tcacatagtt aaaaaaaaca aatataaaaa tattgatgtt
    13321 gtatttggca atctaaaaga aggtatacct cttgctgaaa aaagtattaa tcatggtact
    13381 agaattatca taagccgagg tggaacttac aatatgttaa aagctactta caatatacct
    13441 atagttgaaa ttaaagtaga tgcttatgat attataaaaa gctacaaaga ggtaaaaaat
    13501 tccaacgaac catttggaat aattggattt aataatgtca tctacggttt cgatattata
    13561 gaagaaatat tgaataagaa aataactatg attgaaattg aaaaagaaga agaaatttat
    13621 gatgctatag aaaagtacag aaagaaaggt ataaacacat atataggaga taccactgtt
    13681 gcacatattg ttaagagact taattgtaag ggaatattaa tcgaatctag agaagaaaac
    13741 atacttagag ccattcaaca agcagaacaa atactcgaag caaccaagga tgaacaaaaa
    13801 agaagattac agatagaagc tatgacagat tttgtacatg atggaataat aacagttgat
    13861 aaaaatttta aaataacatt atttaataag agtgctgaaa aaatatttgg tataaaaaaa
    13921 gaaaatgcac tttatcataa tgttgtagat gttatcccaa acacagttct tccacaagta
    13981 gttgagacta tggaacctca aattggtgaa atacaaaata tagggagtac aaaaattatt
    14041 acaaatagag ttccaatcat tgttgatggt cagattcaag gagctgtttc aacatttcaa
    14101 gatagcaagg agataagttc ttttgaacat acaataagaa ggagcttatt aagcaaaggt
    14161 ctatctgcta aatacacttt tgatgatata attcatgcta gtgaaaagac aaaagattgt
    14221 atagaacttg caaaaaaata tgcttgttat gatactccta tgattattac tggagaatct
    14281 ggagttggta aagaactatt ttgtcaaagt atacataact atagtaagcg taaaaatgca
    14341 ccttttgttg ctgtaaattg tgctgccata cctccatctc taatagagag tgagtttttt
    14401 ggttatgagg aaggttcatt tacaggtgct agaaaaaaag gtaaagcagg tgtattcgaa
    14461 ctagcacatg aaggtacaat cttcctagat gaaataagtg aaatccctct tgaacttcaa
    14521 ggtcgattat tgagggtatt gcaggaaaaa caagttatga gaataggtgg tgataaagtt
    14581 attcctatag atgtaaaaat tatctgtgct tcaaataaag acttaaaaaa tatgatgcgc
    14641 aatggtttat ttagacaaga cctttatttt agaataaata tactaacatt atatcttcct
    14701 tcattaaaag aaagaaaaga agatattata aaattatcag aattttttat acataaatat
    14761 tcaagcaaat acaataaaac gcctttaatt atcactccaa atattaaaaa ggcacttctt
    14821 gaacgagaat atgaaggtaa tataagggaa ttggaaggta tgatggaaag ggctgttatt
    14881 gtggaatcat ttgactcgat tttattagaa aaggttagca atgaatatac aaataatata
    14941 gttgataaag ataatagtga ttttgaatgc agtaatttag atttaaaaac tttagaaaaa
    15001 aaatatatct ataaaattta taacgaaaat aatagaaata ctacaaaaac ctgtgaaatt
    15061 ctaaagataa ctcgctccac tctatggcgt aagttaaaag aaatagaaga tgatgtttaa
    15121 aaataataca tacctatata tatcaaaacg ttttatgttt aattttaata cataagacgt
    15181 ttatttgttt tcaataaaaa atacaattat atgataaaaa gaatctttgt aagtgaaatt
    15241 ctcgtcaatc gtacttttaa ttataagtaa tctacagcat ttcaatcgat tattaaaaat
    15301 aatcatgata actaaagata caaactctat atattggcat attaattgct tttataatac
    15361 ctatataaaa atgaaaggga gtgatactag atgcacatct taaaatcagt gaaaaagata
    15421 ccaggaggac ttatggtctg tcccttgtta ttgggagcac tatttaatac agtatgtcca
    15481 caagctcttg aaattggagg attcacaaca tccttattta aaactggtgc aatgagtata
    15541 cttgctctat tttgtctatg caatggagct caaatcaata tcagacaagc tggtaaacca
    15601 ttgactaaag gtatagtact tacagcaaca aaatttataa ttggtgcagt attaggtatt
    15661 attgttagta aatttattgg acctaaaggt ataattggta ttacacctct tgccatagtt
    15721 gctaccatga ctaattcaaa tggaggtcta tttgctgctc ttgcaggtga atatggagac
    15781 tccacagatg ttggagcaat atctatctta tctctaaatg atggtccttt ttttacaatg
    15841 cttgcttttg gagtaactgg acttgcaaat gtacctatag tagcactatt tgcagcactt
    15901 ataccaatac tacttggatt tatacttggt aaccttgatg aagatatacg agaatttctt
    15961 gcacctggta caacattatt aattcctttc ttcgctttcc ctttaggagc agcattaaac
    16021 tttaatcaaa ttataactgc tggactacct ggagttgtac ttggtttagg agttactata
    16081 ataacaggta tgggtgggta ctttgtaatg aaacttatac gtgcagaaca tccagcagta
    16141 ggtgcagcta caggtacaac agccggaaat gcagctggta ctcctgaagc tttagcagct
    16201 atagacccta ctctagctac aatagcagct gtttcaacag tacaggttgc tgctgctatt
    16261 attgttacag caatatgttg tccaatgtta gtttcctatc tagataaaag agaaaaaaag
    16321 aaaaaatcta tttttaaaac aaataaaaat attgaagtaa attaatactt aaggaggtaa
    16381 aattaatgaa tgcaattatt catgcaaatg aaaaagataa tctagttaca tgtgtcagac
    16441 ctttagttaa aggtgaaaaa gtttcaattg atggaaagtc ttacatagta aatgataata
    16501 tacctgtctt tcataagatg gctacagaag atgtaaaaaa aggtgatgtt gtatacaaat
    16561 acggtgaaat cataggaatt gcaacagttg acataaaaac tggtgactat gttcatgtac
    16621 ataacattga aagtacacgt ggtcgtggag ataaaaaata ggaggaagtt aaaatgaaat
    16681 taatgggtta tttaagagaa aatggtcagt ttggtattcg taatcatgtt ttaattattc
    16741 caacatctgt atgttcaagt gagacagcta caagaatagc atcacttgtg cctggagcaa
    16801 tagcaatccc tcatcaacat ggatgttgtc aaattggttc tgatatagag cttacagcaa
    16861 agacattaat aggatttgga aaaaatccta atgttgccgc agttttagta gttggtcttg
    16921 gatgtgatgg tattcaagca aaagaattag catctgaaat agctacaaca ggaaaaaagg
    16981 tagattacgt tgttatacaa gaatgtggcg gaacattaaa aacagtttct aagggagctg
    17041 aaatagtaag taaaatggct cgtgaagtat caaaagaagt aagagttgaa tttggtatga
    17101 gtgaaattac attagcactt gaatgtggtg gttctgaccc aacaagtgga atagcttcaa
    17161 acccttcaat aggcgttgca tcaaaccttc ttgtagatga aggtggaagt agcatactaa
    17221 gtgaaactac agaagtaatt ggtgcagaac atttgttggc tacccgtttt gaggatgaag
    17281 aaatgaaaga taagtttctt aaatttgtaa gtgatgtaga aaaaagagca attgctatgg
    17341 gagaagattt acgtagtggt caacctactc caggcaataa agctggtgga ctttcaacaa
    17401 ttgaagaaaa gtcacttggg tgtatgtata aggcaggcaa taagccattc aaaggtgctc
    17461 ttgaatatgc tgatattgta cctcctgata aaaaaggatt atattttatg gatactccag
    17521 gtcaagatat agattctatc actggtatgg tagcaggtag tgcacaaatt gtaatctttt
    17581 caactggtag aggtactcca acaggaagcc ctatttcacc agttattaaa atcactggga
    17641 atagtgatac atacaacaaa atgccagaca atatcgatat aaatgcaggt agaattataa
    17701 cagatggtgc taaaatagca gatataggtc aagaaatatt taatgaaata atagaagtct
    17761 gtaatggtaa acataccaaa actgaaagtc ttggtcatag agaatttggc atttacagaa
    17821 tttcatctac attttagatt gtaacaaaat atcttttata aatataatac ttatgtagat
    17881 acataagcta aaagttcttt ctaatactat aaaaattttc tatactgaaa aggaactgta
    17941 tcaaaattat tttgaaaaca gttccttttt taacctcttt tactcaaaat attataaaat
    18001 aagatttaaa caataaacgt atcttgatta atctctaaga agacataaat aaagctaaat
    18061 gtaaagtggg ataaataaaa tttaatatct ttgtatgaaa agatatttgg tataatatgt
    18121 ctgagagaat tcttatttgt aaacaaataa aggatggagg atgaaacaaa agaatgaact
    18181 ttgaaaacac aaaaaataaa aaaataatag ctactttact attgacactc cttttaatcg
    18241 tgtcattaac atgtgtcact tttattatga atctaaataa agtaacacat aatgaaacag
    18301 aacgatatct tgtagaagtt tcacaaagta tttccactac aattaatgtg aaatgtaaaa
    18361 gtatatttag gacactatat tctatgggag atacttattt tgagattaat gatagtgaaa
    18421 aagaagtcaa tagatactta tcaaaaaaat taaaattatt tgaatttgat tggttaggta
    18481 ttatagattc taatggaaat tttgtaagtg cccttaaaga tacaggtaat gttaaaggac
    18541 aagactttta tagaaaagcc cttcaaggca aagaaacaat tactcctgtt aaatcacaat
    18601 ctggtacata tggaatttta tattctgtgc cactatacaa tggcgataaa attgttggag
    18661 ctgttgttgc ttggaatact attgatacta tgagaaattt attaagtgta gaaagctttc
    18721 atggtgaagg attttcccaa attatcgacc aagatggaaa ttttattgta agttcagcaa
    18781 acaaaaatgc tccaaaaaat gtgatgaatt tttttgacat gattaaacac caaggaaaac
    18841 aggttgataa aaataaactt gaagaaatga aaataaactt aaaaaagaac aaaactggtt
    18901 ctatgtatta tacattaaat gatggtattt ctaaggcaat gcactatctt ccattacaag
    18961 aagaaaactt atacctcctt tcaattgttc caacaaaaac agcaagtgca cagtttgatt
    19021 tacttctaaa aaaagcaatc ttcatcaata ttttaattgt tatactattt ttattgctta
    19081 ttatattgat tatgtttata aatcaaaaga acagggcaca acttataaaa atcgcctatg
    19141 tagactctgt aactaaaggt tacagtaaag cacgttttga tattgaagct aaaaaaatta
    19201 tacagtcatc ttcatcagaa agctattcat tggtatctat aaatattcag aaattcaaac
    19261 ttattaatga ttcttttggt agtgaagcag gagataaaac attaaagtac atacatgatg
    19321 tcattagtaa ttatctagaa ccaggagaat tgctttgtcg agtttctaat gaccagttta
    19381 atgtacttat taaaactatt cctcaaaaag aaattcttaa gtacatagaa aatatcacca
    19441 atgatattaa tagtttcaat aaaaatgaag aatgtaaata ttttatagct ctatctatag
    19501 gtgtatataa aattgatgac ccaaatctac ctttagtttc aattcaagac cgtgcaaatg
    19561 ttgcacgaaa aaataataaa ggaaacattg gaaatcatcc actttataca tgtgtttttt
    19621 atagtgattt agagcgttta aaaatgatta aagaaaaaga aatggaaaat agaatggagg
    19681 aagcattaaa taataacgaa tttatagtat atcttcagcc taaaataagc ctcaaagata
    19741 atactattgt aggtgcagaa gcattagtaa gatggcaaga cccaaataaa ggccttatac
    19801 caccagacga gtttattcct ttctttgaaa aaaatggttt tattacaaaa ctcgaccact
    19861 atgtttttga aaaagtatgt agtatgataa gaaaatggat agataatgga gaaactccaa
    19921 tacctgtttc agttaatttt tcacgtgcta atttaaataa tatagatttt ttagaaaaat
    19981 acagaatgct tcgtaataaa tacaacattc ctgctaagct cttagaaatt gaattaactg
    20041 aaacacttgt atttgaaaat ctccaaaaat taattaatat aatagaccaa atccatgaag
    20101 aaggttttca atgttcacta gatgactttg gaagtggata ctcttcactc aatatgttaa
    20161 aagaaataaa agttgacaca ttaaaacttg accgtgcttt ttttagctca cctaatgcag
    20221 ataatattgc tgaaaatcat gtaatagaat ctatagttaa tttggcaaaa aaattaaata
    20281 tgaatagtat atcagaaggt gttgaaacaa tcttgcaaat ggattatctt aaaaaaatta
    20341 attgtgatat gatacaagga tatgtttttt caaaacctct acctcaagaa atatttgaga
    20401 aaatgacttt tggaaaaaaa ctagaagaaa ccagtatgga tagttccgtt taaataaaat
    20461 aaaaaacttg tctctataaa ataattttaa tcattttata aagacaagtc tttttatttt
    20521 ccaatttaat tttattttct aaatttctta cactcggctt gttttaaatt ccctttttaa
    20581 gaataataat acgattatca tagcaataaa atctgcaact ggctgtgcca accaaacacc
    20641 atctaaatta aattgaattg gaagtacaat gattaatggt ataaataaaa taaattgtct
    20701 taaaagagtt aaaaacattg aatgtttaac tttacttata cactgaaaat acacaggtcc
    20761 tacaatagaa atccccatta taggcaatgt aattagattt aggcttatac ctcttactgc
    20821 tatatccatc aaatctgaat ctttattaaa tatacctaca aatacttctg ggaaaacttg
    20881 tactactaaa aatccaaatg acaatatgac aactgacact atgatagata atattaaagt
    20941 tttctttgca cgctcatatt gttttgcacc ataattgtat gatattatag tttgcatacc
    21001 ttgattcaat ccaaacacag gcatcataaa catcaataaa atagatgtaa gtgctgtcat
    21061 agctccaata gctaaatctc caccataaac ctttagagaa ttatttgtaa acatgtgagt
    21121 tatacttata gctacttcca tagcaaaagg tgtcattcct attgcaaata ccttctttac
    21181 tatatcttta ttcaatttta agttacattt ttttagtttt aaattggact tccctcttat
    21241 aaaataatgt attacccaaa aaaatactac tatttgacag agtactgttg ctatagctgc
    21301 tccttttata cctaaattaa atacaaatat aaatataggg tccaatatca ggtttaatac
    21361 acatcctaca atcattgtcc ttgctgctaa ttttggatta ccttctgccc taattgcatt
    21421 attaagagaa aatgcaacta aattaaatat acttccaacc aatatgacac tcatatagtc
    21481 ttttgcataa aatattgaat ctttacttgc acccaaaata aaaagtatct cttctaagaa
    21541 aactaatcct aatatagtta taattaaaga gataattata gataatgtca aagcattacc
    21601 taatatcttc tctgcttctt ctctatttct ttcccctaac tttatagaca atcttgtgct
    21661 agcacctact gataccaaca ttccaaaagc tattattaga gtaaatactg gcatagttac
    21721 accaagacct gcaatagcta tagagccaat accttccata gaaccaataa aagctctatc
    21781 tactacatta taaagtgaag ttaccatcat tgctattatt gcaggtatcg aatacctaaa
    21841 taataaaatc catatttttt catgtcttaa tgcttcttga ttctccatgt tgtcaccatt
    21901 tacaccatgt cataagttat acaaattggt ttatttgttc ttgggtctgt tgctatttct
    21961 gcatcaatat taaataattc ttttagatta tccttcgtca taacatcata tgcatcacct
    22021 tcacaaacta ttttaccttt cttaatacct atcatgtggt cagcaaatct tgctgcatta
    22081 ttaagttcat gtattaccat gacaatagtt cttccttgtt ttttatttag ttcatctaat
    22141 aattttagta tctccaattg atgtgccaaa tctaaatatg ttgtaggttc atccagtaat
    22201 aatatatcag tttcttgtgc caaagccata gctatccatg ccctttgtct ttgtcctcct
    22261 gataaatctt ccatatttct atccttaaat tctgcaattc cagtaacttc taaactccaa
    22321 tttacaatct ctctgtcttc tttactagaa ttaccaaatc cacttttatg aggaaatctt
    22381 ccataagaaa ttaattctga aacggtaaga ccactaggag cttgagggct ttgtggaagt
    22441 acagccatat cttttgctat ttctttagac ttctttttgt agatgtcctc tccatttata
    22501 tatattttcc cactattagg gctaataatt ctacctatag ttttaagtat tgtcgatttt
    22561 ccacaaccat tagcccctat gatggtagtt atttttcctt ttggtatatt taaatttaaa
    22621 ttttttataa tatctgtgtt tccatatgat acatttagat tcttagtatt tatacagttc
    22681 ataacaacac cctttcctaa tcatttacca acattaaata aataaagtac ggcactccta
    22741 ttatagaaac tactatacct actggtattt ctataggtgc tagaagattt cttgatatag
    22801 tatctgctat taaaagtaat aatgttccta tcaatgctga tactggtatt aatcttttat
    22861 gctttggtcc tattattttt ctacctatat gtggagctat taaacctaaa aatgaaatac
    22921 tacctgctac tgctgtcgat acaccagata aaattactgc atatattata agtcttcttc
    22981 tttgtttttc tacatctaca ccaagactta tagatacttc atcacccaaa tttaaaacat
    23041 ctatatattt agatttataa atagttatac ccataaaaat cactataaat ggaagcacag
    23101 ctattacata tttccaactt gttccccaaa tgcttcctga tgtccatgcc ataaccctat
    23161 taaattcttg tgttgtaaat ttcaattgga atatcgtcaa aattgaagta aaagctatgt
    23221 ttacacctat accaataagt agtaatcttg acgaatttat accacctttc catgcaagag
    23281 aatatataag aaatgctcca aataaagctc ctataagtgc aacaataggc attgtaaata
    23341 cagtcaagtt actcatacct tcatatacat taccattcat aaggtatata taaactacta
    23401 caaacaatgc agcacctgaa tttattccca aaattccaga atcagctaaa tcattctttg
    23461 taactccttg aagtattaca cctgctgttg ataatgcaga accaaccaaa agtgctatta
    23521 ctattcttgg taatcttaat ttaaatattg caatttcatg attacgctga ccctgcccta
    23581 aaaaagtctt tataacatcc attggttcta tagaaaatga gcccatattt aaactcaata
    23641 aaaaagtgat aaaaataagc accattaaaa ttgatataac aagtaatgtt ttagaattta
    23701 tttttataat atttttcata gtacctattt ctcccccttt ctaacaaggt atataaatat
    23761 tggcactcct ataagtgctg ttatagaacc tactggtgtc tcatatggag gatttatcat
    23821 cctacttaat atatctgtat atacaagcaa tacagctcct aatactaaag agctcggtat
    23881 tatatactta taatctgaac ctactattcc tttagctatt tgaggaacta taagacctat
    23941 aaaaataata ttaccagaca ctgacacaga tgctccagtc attaatatta caagtactat
    24001 acatataaat ctaacaaact tagttttttg tcctaatcct attgccactt cttctccaag
    24061 acttaatata gttattcttg gagccataat aatagctaaa ataaatccaa taccaccagc
    24121 tacaaatagc aattttatac cttcccactt cactcctaca agccctcctg atatccagaa
    24181 acttaattgt tgagataggt taaaatacat agatattgct gatgccaaag aaattagcaa
    24241 tgtaccaaga gcgactcctg caagagctaa ttttacatta ttaactcttc ctcttgattt
    24301 agaacttata aaatatataa ataatccact tatacttgct ccaataaatg caaacatcat
    24361 tacactaaaa cttccatgta taagttgagg aagttttttt tgaagtacca gtgatatcga
    24421 aattgcgaat gtagctcctt gtgtaattcc tataacagat ggttcagcta taggatttct
    24481 cgttattcct tgcattattg ccccagaaac tgctaaaaac cctccaacaa gtgcagctgc
    24541 tattgccctt ggaagtctaa catcctttac tattctcata tttattccat cttccatttt
    24601 tatcaaacta tctataacag ttgatagatt catatctttt gccccaatag ttatggataa
    24661 tacaattcca aatacaagta caactatact tactaatatc aacaaataag ttatattttt
    24721 tttactcagc ttagtcaatt atcctacctc cttttccctt ttaatcgtca gtataatatc
    24781 atctattaag tattttaatt ttaagattta ttaaaaatag gtgtgaaaaa tgaatatcat
    24841 tatctcacac ctactaagtt aagaattact ttgttaattc atttttaact gattctaata
    24901 ataactcttt tccaattgga ttgtatgatt gactgaaaaa tggagcagca tcaagtattg
    24961 ttacattacc atcttttact gctcttattt gagcccagat agcactattt tctaaatctt
    25021 ttttatctgc ttctgtagca attacaacta tatggtctgc atctatatct gttaaacctt
    25081 ccatacttac cattggtagt gttattccat cctgttttgg catattctta ggtctagcta
    25141 atttcatatc atcattgatt agagtaccta ttccaccatc actaaatacc ataaattgac
    25201 ctgaacttgc caacactggt aaataagttt tttctccatt cttttctata acttccttac
    25261 ctaactttgt agctttttca tcatacttct gtagccaact tttatcttct tcttctttgt
    25321 caaataactt tgaaacatca gttaacttgc ttctccagtc atttgcataa tctttcatca
    25381 taactacagg agctatttct tttaattggt catatatttt ttcttgtctt tgactcatta
    25441 ttattaaatc aggatttact tctaatatag cttccatatc catagtatcc atcattgagt
    25501 gcccaactat ctttacacct ttaagctctt ctcttatgta acttggtaat ttatcagttt
    25561 catatgagtc tacatttgct gttgcaacag gcttataacc tgctaataat agttcttcac
    25621 tacttcctga tatatcaact atttttttag gatttgatgg tatcttaacc tctcctttaa
    25681 cagattgtac aactcttgtc tctgatttat ctttatcctc tgtattctta tctgaacatg
    25741 ctgtaacaag tactaacgta gttataatag atataaatat tgcaagtgat tttatttttt
    25801 tcatgattac ttctcctact tgtttattat acttacatga tattgattat cataagcaca
    25861 tatttattat agcatttttc tatttttagt caatattatt ttgtaaaagg ataaaaattc
    25921 ctaattaatc ttaattatat atagcttaga taaggttttc ttatattaaa cagtattttt
    25981 tttaagaatt ggacacaaat acaatgactt ttcagttaaa caatgataaa atgcaattta
    26041 tagataagga atttagtagg gaggattcaa aacatggtac atttagtata ttgtgataat
    26101 gcaggaaaaa aaggagaaaa ggtcttagat aaaatcttgg atggaacaaa aacaatggtt
    26161 attagaggtg ctgctggtag aaaaatacca catagtagag tatttgaaga tgagttactt
    26221 tattttatgg aaaaaggaac tgcaaaaata tctgcaaaag ccactgttaa aaaggtagaa
    26281 aactatgtta aattaacaga agatgaaatt tctaaaattt taaatgaaaa tcaaccaaaa
    26341 ttaaatttat ctgaaaagca aaaagttcgt tggcataaaa aatgtttatg cttagttgaa
    26401 tttgaaaatg ttgaagaaat aacacctctt gattttgacc atcaaggaaa tatggatgat
    26461 tggcttattg ttgaaaaaat tgaagatgta gttgttggta ctagtatacc ttacaattat
    26521 gagaagtcta aattctaaag tatcaataac ataatactat attgctaaac tctacttaaa
    26581 taaaaaaaga gtgcatttaa taattataaa aattattttt atacactctt ttttaataat
    26641 aattttaaat tacttacttt ctaatctttc atatacatct ataaattctt ctgctaattg
    26701 ttgaaaattc atagctgtca ttaaatggtc ttgtgcatga ataagtataa cacttatttc
    26761 agttttatta ccattagctt cattttgtat caattctgtt tggaatttat gagctttatg
    26821 aattgattct ttcgactttt caatacattc tctagcttta tctatattcc cgctctttgc
    26881 ttcttttatt gcttcaaatg ctagcccttt cgcatcacct gcaaatccaa ttattccaaa
    26941 agatatctct atcatcttct catccattta ttcgacctcc taaatatctc tatagcttga
    27001 tatgactaat ccattctttt ctaaaaattc tttgactttt ttagatgtaa gtatcttgtg
    27061 ctctttcgtt ctatcaatag cataagatgt agaatttaaa atataatcat ctaaaaaagc
    27121 tgggtgagac ataatatcca ctacatcata cttcattatt tcgtctatat tttttataaa
    27181 atattcttca cttacatttt ccttataaaa actatctatt aaaggaacta ctttagaata
    27241 ctctaaatta tattcaaatc cacctcttat tggaagatta tacttggtta caatcttctc
    27301 tattactggc tttaaactta ctaaagtatg gatatgatgg tgactatcta aatgagatat
    27361 atctattcca agccctttta ccctatctat ttgagcacaa agttctctat aaatctcatc
    27421 acaatccatt ttttcaataa tttcgtctgt aatcctccta ataaaaaaac cgtctttatc
    27481 aacaatagtt tttaagttag ataataatgg tctaccacaa cttaaagtca tatgaactcc
    27541 acaatttaaa gttttatttt ctttaagtaa gcctactcca tgttctaccc caggcatatt
    27601 tgccatcata gaagtagacc taactatacc attattatga gctgaaatta ttccatagtt
    27661 cacagcttca caatatccaa aatcatctgc atttattata attttagtca taccataacc
    27721 tactttctat agacaactat ctattaaatc taggaagata atctttatga gcttccaaca
    27781 tttcgtctaa tatagttttt gctaagtcat cagatggaat caatgggttt atacaaagtg
    27841 ccaaaagagc agattcataa tcgccatata cagctgcttt agctgccaat acttcaaatg
    27901 atttgatttg acttactaat ccatgcacag aatctggtaa atatcctata cttattggtt
    27961 ttggaccatt ttttgttatt acactactta cttctactgc ttgttcatct ttaaaatctc
    28021 ttatagaacc cttatttaat gtattaacta cttggatatc ttttttgtca ttgtatatag
    28081 aacttattaa attacaagca gcatcactat agtaagcccc tcctcttttt tcaagttgag
    28141 gtggttttac atctaatttt tcatccttat ataattcaaa taattgttct tccaattctt
    28201 taactacttg acctctagct tttccctctt taaactctct caactcatcc tcaagcattt
    28261 ctttagtttt atagtagtat ctatggtatg gacaaggtat tgcacccaaa gattttacaa
    28321 attcagcatt aaaatcaatt gcctttatat tttgcatact tatatctgct ttgacaaatt
    28381 tatcaattgc ttcttttgta acatcttctc catctaaaga tacatttaga ccataaacca
    28441 tatgattaag acctgcaaaa tccatactta ttctgtcact ttctacatta aatagtttgg
    28501 caacatcatt ttttaaatga attggcacgt tgcatagacc tatatatctt ctaaaattag
    28561 tatatttgaa tacagcctct gttattattc ctgttggatt tgtaaaattc accatccatg
    28621 catttggaca taattcttcc acatctttta ttatatcaaa tataactggt attgtgcgaa
    28681 gggctttaaa taaacctcca gcaccattag tttcctgccc cataactcca tgagataaag
    28741 gaatagtttc atcttttatt ctcgcatcta aaagacctac tctaagttgt gttgtcacaa
    28801 agtctgcatc tcttagagct tctcttctat ctaaagtaag atttattttc atatctactc
    28861 cagctttttt taccatcctc ttagctagat ttccaactat ctctaatttt tcttttccag
    28921 cttctatatc tacaagccat aattccttta caggtaactc ttcatatcta tttataaaac
    28981 cctctacaag ctcaggagta taacttgaac ctcctccaat tgtaactatt tttagtccct
    29041 ttttcatctc attataccat ccttacctat aatttaatat actctacaaa accccataca
    29101 tttatttaaa actctataca tttatttacc gaaataaaac tacctagttg ttaattttca
    29161 atttattagc ttccattttt tcagacgcta tgacaaatgg aatatatatt aatattgata
    29221 atattaaatt aaatagtgct aaagctccgc cagtccagtc acctgtagcc attgctccac
    29281 ctaatatagg aggtgttacc caaggtattt taggtaaaat tactggtgct actaatccaa
    29341 aatctattgc taagtatgat acagcactac atattactgg tgcaagtaca aatggaatca
    29401 taaatatagg atttaaaact attggaagtc caaatatcaa tggttctcct atgttaaata
    29461 ttgcaggtgg accacctaat actatcatct ctttacgtct tcttccagct attaataaag
    29521 ctactattaa tcccaatacc attccagaac cacctaaata tacaaatgca tcaaagaatg
    29581 cacctgcaag tacatggtat ccttccgttg ctcctgcttg agccaaggca gcattttctc
    29641 cacctaattt cataagtata gtttctgtaa aaggaagtgc tatatttgca ccatgaagtc
    29701 caacagtcca aagtatatga attataaatg caataagcat tgtagaacct agactatcag
    29761 ctacatttga tagtggtgct cctaaataag tatttaaaat atctgttaag aaacttccat
    29821 tagaaagcat ttttataaat attcctatta cagtaaatat cattatagtt aacattcctg
    29881 gtattaactt tgcaaatgat cttgaaactg ctggtggaac tccatctggc atttttatag
    29941 ttaatttctt taccttagat aatcttacaa atatctcagt tgataataat cctattaata
    30001 tagaagagaa taaagctgct gtacctaagt aggtttgttg tatcattccc catccttcaa
    30061 ctactgtagt tcctccctca ggaactattt taccaatttg tggagccatt ataaaaaaga
    30121 tagataaagc tataagccca gattgtaacc catcactctc ataagatttt gctagaaaat
    30181 aagcaacacc tattactaag aatactgcca ttgtacctat tgctccccac caaaggtcgc
    30241 ctccaaatgt ggtccagttt tctccaaata tacttgccat caaatttttg tatgcttcca
    30301 atggtaagtt atttatcaat gttcccaatg cacctaccat agttataggt atcattacta
    30361 taaatgcatc tcttactgct actaagtgcc tttgagctcc tattttagca gctacaggca
    30421 ctatatattt atccataaat gacatgaatt tttccatttt acatcctcca gtacaacttt
    30481 attttgaact atttaactct acagctctat ctaacacagc ttttccatta catcttccat
    30541 aatccatcat atttataact tctactggaa tattatatgg ttttgccatt tcagttgctt
    30601 ttcctaatac atatctaact tgaggcccta aaagtaagac atcacaattt tctatttcat
    30661 tttttaagtt cacttctgat atagcccata tttttgcttc tataccgttt tcctttgctg
    30721 catcctccat ttttgtaact aataaactag ttgacattcc tgctgaacaa gctaacatta
    30781 tttttatcat tttttcccct ccaaattaga ttcattaaat attttttaag tcataagttt
    30841 tttaaactat acatttaaaa tatgtttttc tattaatttt ttcttaatgt atatatctta
    30901 tttttaaatg tatatacatt taattcttga tattattata taccaaatgt atatacattt
    30961 caagaaaact ttttcatttt atttaaattt tattcgacaa cagcattcaa agttaatgta
    31021 tatccatagt gatatgtgtg tgagtactca aaaatctcac tattttgtaa cttacaaatt
    31081 tggcttatat ataaaagtgg ctctggttct tctaatttta gattatccat acactcttca
    31141 ttcgatatta cagcttgtac ttctctctct gacatggcta tcttgtattc acacacttct
    31201 tcaacaaaat tataaagaga attttcacaa tgagtcttat ttaaatttgg aaataactta
    31261 actggcatat aagtaatcat atatgaaact cttgcatcat tggctaatct tactcttttt
    31321 atttcccaaa cattttcttc taaacttata ttcaacttac ttgataaatt ttcatctgcc
    31381 tgaactattt caagagatac caatatatta gatactttat agcctttact ttccatctct
    31441 ttagtaaatc cacttacaga cgatatatta tcatatatat tatttgcaat aactatacta
    31501 cctacacctc tttgtctagt tatatagcct tctttaacta gattattaag tgcagacctt
    31561 acagtcattc tagttacatt aaattcttca ctcagctgtt tttcagaagg gattaaatcc
    31621 ccttctttca aactgtctga atttattaac tctctaacat gattttctat aactttataa
    31681 attggctctt tcatttaatt cactccttat taaatctttt atataataag ataattttat
    31741 atacaaacaa agaaaatgtc tatacattta agataattta aattttattc agaaatcttg
    31801 tacaactttc cgagttttaa taaagatata aatagactta tcattaatat accttttaat
    31861 atacttgata aggttatact ccaccataca ccatttagac ccaacatttc tggtctagat
    31921 ataaagtaag ccaatggtac tcttgcacca gtcagtatag taattataat agaaggtata
    31981 taggttctcc ctaatccttt gaaagctcct gttgttatta tttctaaaca cataaaaaat
    32041 tgagaatagc ctaatatttt aagataaact gctccttttt ctattgcctc actttcatct
    32101 ataaaaatag aaaatatact ctctcctgca aacactaata ctaaagtagt tattatacca
    32161 agtacagaag atacagttat agctattttg caacctttat ttattctaga atacttcttg
    32221 gcaccataat tttgtccaac aaaactgcct aatgcaactg atagcccttc agccgtcatc
    32281 caggaaattg cttctatttg tgagcctact ttttgtactg caacagccac aggcccccat
    32341 gatgctacta ttactcctaa tagcattgag aatactgtaa acataccact ttgtattgct
    32401 ataggtaatc ccaatttata cagaacttta taatatttag tctctatttc tttaaatagt
    32461 cttaatttaa aatattcttc tttactttta actattatat atataaaaca tgtagtaact
    32521 actatctgag caaatacagt tgcaatagca gctcctgcaa caccaagttt tggagcaaat
    32581 ccccatccaa aaattaatat agggtcaagt ataatattag ttattaatcc tactgtatta
    32641 atacgaaaag gagttttact atttccaaga ccattgaaaa ttgcagtaaa tactggatta
    32701 ataaaataaa ataccatacc tagagctact attatcaaat actgtctaga catggttata
    32761 acttctaagt ctcctaatct aaaaaaacct atcaattgtt tattaagtac tattaaagaa
    32821 attgtatata ttattgctag tataatattt atttctattg ctgacttcat atatgattta
    32881 gtagtgctta tattatttgc accaatactt tgtgctactt ttatctctcc acctactttt
    32941 gaaatcataa taaatgccat tgcaagccat ggaaaaaaac cagctgttcc aactgcagct
    33001 actgctttac ttccggcttt accaacccat atcatatcta tcatgttata ccccatctgt
    33061 ataaatgaag tccccattat tggaagtgat agcttaagta atttttctgt aattcttcct
    33121 tctgttaaat taagtctgtc tttcattttc cacctctttt atattaaatt tatttgctta
    33181 tatagctaga ttaatcattt tatttgattg agttcactat ccttcaattt atattatcat
    33241 atctcaggat tgcatctcaa tcatacttat gtttaagata cagtatttta cctatctatg
    33301 ataaaattaa tacttatgaa tataaatcta gccatttcat caatacatat tctaaccctt
    33361 aacttaactt caattaagta tacatatcgt aaccaattag aatattttat acggtatctt
    33421 ccattaataa acaaccaata tcaatgattt atatataatt ttatttatac ataaaatagt
    33481 atactataat aataaaaaaa ctatatttga atgctttaag catgtttact aattgtaatt
    33541 tccacacaaa tttgataata tatgaatata ttatggtgaa atttatagat atatattaat
    33601 tatggggaga ttgtgggagg ttcttttcac aagacattta agctatgaaa ataaataaaa
    33661 aattcaatag agttgatatg caggtttcaa tacttactgc aattattgta ataatatctt
    33721 cctcatgtgt atattttttt aattatacac taacttataa gagcatgata tactctttaa
    33781 gtgagaggtc aaaacatatc tatgaatatg tagaaagaaa tattgataaa aataccttta
    33841 ctgaaattaa ttctaaagaa gaccaatcaa aaaagtctta taaaagttct aaaatccttt
    33901 tagaatctgt aaaaaatact actggagtca tgtatctata tactgctaaa aaaacaaaag
    33961 atggatcact agtatatgtt gttgatggtt taaattcatc taggagtgat tttagaaatg
    34021 ctggtgattt gatagaaaaa gagatttggg gagaccttaa caaagcatta gataatcata
    34081 tattgttacc taaaaagatt aaatcaacat cttggggata catatttata tcgtattttc
    34141 ctattcacaa tgatgttggt aaggttgttg gagtaatagg aattgaattt gaagcaaaac
    34201 atcaatacaa tacatttaaa tttataagta tagttacacc aataatagca ttattaactt
    34261 gtctaatatc agcattgatt gcagtgattc tatttaaaag aatatctaat cctacatata
    34321 ctgattttgc taatacagat tatttaacag gcctaaaaaa tagaaatgct tttgaaatag
    34381 atatgaataa cttaaacaat tcaaatctaa agaatctaat atctatcata tcaatagatt
    34441 tagacggttt aaaaaagatt aatgataaat ttggccatca gacaggagac ttatatatta
    34501 agcaagcaag taaaattata caatatgtaa taaataaaaa atatatagtt tatcgtattg
    34561 gaggtgatga atacataatt attctaaaaa atttatctca caaagaaata aataatatta
    34621 taaataatat taatttaaaa atagttgaag aaaactcaac taatgaatta aaattatcaa
    34681 tgtctattgg atatgctatt tttgatgaaa aagtagatgt ttctttattt gatacatatc
    34741 gtcgtgcaga ttctcaaatg tatgataaca aaaggaaaat taataaacaa tatcaataat
    34801 aaatttaaaa agtctattct ttaaataata aatcacaaga atagactttt ttaaatttgt
    34861 gtttttataa gataaattat ttaattaaag tttgttcttt tttctaaacg attctttata
    34921 aattcatata ttactttcac taatatattt gtgactaata ttaacacagc tagtgctgct
    34981 gcctgtgcag tactcccctt atcatctagt tgtacaattg atattgcagc tacttttgaa
    35041 cttgcagtat atataaaaac taatgctgat attgtcacca tggagtttag gaaaaagtac
    35101 ataaagttct ctaaaactgc tggcaaacaa agtggtatag ttacgttctt aagaattgat
    35161 gcgctgttta ttcccattga tttagctacc atatctaatt ctttatctac tttcttaata
    35221 gcagtagtag ctgttataaa tgctactgaa aagaaatgaa ctatattaca tataaccatt
    35281 ataagtaatg ttccatataa tccattaaac aaattcttaa tgtatattgt atccataaaa
    35341 ttaaactcta atttattaaa aaataataca taagctatac ctaaaactaa tcctggcaat
    35401 gccataggca atgtacttaa aaaataccca aatgatttta aatatggagc tttttctgtt
    35461 ttttcattca aatatgcaaa taagaaaaca aagctcgtcc ctagaatagc tgtaaatagt
    35521 gacattttta ttgaatttat aaatggttta atcccatcaa tactgttttt catagaaaaa
    35581 tgttccagtg tcagtcttaa atcatatggc caaactgata ttaatgaaga taagattaca
    35641 gttaacatta cagctattat accaaatgca ataattccta caacccctcc taaaatacta
    35701 tctcttaact tattagtttt aaccttatat ggaattgact ttgaagttat atatgaattt
    35761 tgattttttt caattttttt agttactata aacgatatta ttgcaggtat taaaagtaat
    35821 attgatacag ttgctcccat tggcatattc tgttgcccta taatttgctt atatacatct
    35881 attgataata cattaaactc tcctcctatc acctttggag ctccaaaatc tgtaaaacat
    35941 aatgtaaacg atacgaatgc agaacttatt aaagagtatt ttatacttgg gattgttatg
    36001 cttataaatt ctcttatctt gcttatcccc atagtattac tagcttcata tagtctataa
    36061 tcggttgaac ttagagatat ataaagtatt tgaaatgatt gaggaaatac atatataatt
    36121 tgtgccaata ttattcccac tgctccatat attggaacat gcactccaag taaattagtt
    36181 accaaacctt gatttccaaa taaatatact agacctattg catgcatcat tgttggagca
    36241 aacataggaa acatagctaa tgatttaaaa aaacctttaa atttaatctt tgttctagtt
    36301 aatgcatatg catatactaa tgcaagtgtt agtgatatta cagttgatat aactgataca
    36361 aatactgtat tttttagaga atttaataga gattcagaag ccaagtattc ttgaaagttt
    36421 tgtaatccta caaagactcc atctttatct aaaaaagcct gtacaaatag atatattata
    36481 ggtgctaata aaaatattac taaagacata actaatactg taagcataca tttttttata
    36541 tctaaattca tttttgattt aagacttata cttttagaac ttatagcatt attattcata
    36601 cacttatgct cctttaactt ttggatactt taaatagtat ttttcgtcta tacttatttt
    36661 tacaaggtca ccttctacta aatttaaatt aacccactct tttatagaaa catcacttat
    36721 tatgaaattt tcatttaatt tatcttttat ttcaactctc aataaattcc ctctaaactc
    36781 tatatttgat attatccctt ggtagttttc ttttgtcgat ttctctattt gaacatattc
    36841 tggtcttaca gttaatatag aatctccatt atcaaaacag tttgtatctc ctataaattg
    36901 agctgtaaat aggttttgag gattttggta tatttcttct ggagtcccta tttgcattat
    36961 atctcctcca tttagaatag ctattttatc tgccatagtt atagcttctt cttggtcatg
    37021 tgttaccatt attgtcgtta tattaagttc tttttgtaat cttttaatgt ccattctaag
    37081 cttgtgtcta acctttgcat ctaatgcaga cattggttca tctaaaagta aaaactttgg
    37141 ttcaagagct aatgctcttg ctattgcaat cctctgttgt tgtccacctg ataatgcttt
    37201 agggtattta tgagcttcat tagtcaatcc aactgtttca agtacttcct taactttatt
    37261 atctattttt tcttttgaaa cttttctctc ttttagtgga aaagctatat tgttataagc
    37321 agtcatattc ggaaataatg catatgattg aaatactata ccaaaacctc tctttgatgg
    37381 ctctaaattt gtaatatctt tatcctggag aattatagtt ccactattta catcttcaag
    37441 acctgctatt attctaagta atgtagtttt accacatcca ctaggtccga gtaaacatag
    37501 aaattcccct tcttcaatat ccaaactaat attatttaaa accttttttt ggtcataact
    37561 tttaaacaca ttatttattt ttaagtaact catatcttat ctctccttat atagttattt
    37621 ttttatttta aattttatat cctacttttt cttctaaaat tctttcatct aaattattaa
    37681 tttaaacttt acttactttc agattttgaa ccgtattttg attcccaagt tttaagtact
    37741 gtacttctgt ttttggcagc agagtttaaa tcaacttctt ttataagctg ttcaaatggt
    37801 tgtttagaat aaccttttgg aactggatta tctgtcttta tggaagtaac tgctacttca
    37861 tgtgcatatc tatccatagc tgaatcactt attgcccaat ctaaaaatag ctttgcttct
    37921 ttttttatat tatctttttt aactaaggca tttgcttcta agtcccaacc tgaaccttcc
    37981 tttggaaata caacctctat aggttctcca ctagataatt gttgtgtagc tctatatcca
    38041 agtgtaatac ctattggata ctctccagag ccagctaact tcgcaggttt tgaacccgag
    38101 tgtgtatatg tggctatatt tttatgtaat ttatccatgt aattgtaagc atctttttca
    38161 cccatcattt gaataagccc tgatactgta agataccccg ttccagatga tgctggattt
    38221 ggcattgtta taagtccttt atattctggt tttattaaat cttcataact ttttggaata
    38281 tctataccta gtttcttaag ttccttataa ttaactacta ttgctgtctc tgggacactc
    38341 attcctaccc aagatacatc atcttcagta tctttaaatt ttttatcaac tttatcataa
    38401 tttttaggct tatatgcttc aagtgcacca tcttttttta gctctatcat gttcattgct
    38461 gataatcccc atataatatc tgcttgtgga ttatcttttt ctgctaatag ttttgaagct
    38521 attacaccat gagaatccat aactatctct acatctatat tgggatactc ttctctaaaa
    38581 ccttccatat aataagctat ctgctcttcc tctagtgctg catatattgt aagtttatta
    38641 tttccttttc cttgagtagt ttgtggtgtt gttgatatgc aacttgtcat aaatgtaatc
    38701 actacaatgc acagacaagt aaatataaat tctttcatat cttccccctt tgtgtttttt
    38761 ccttttttta tttgtttttt attgtttttg cttatgttta tataatacac ctaatttgtt
    38821 aatataaagt taagtgtaaa ttaacagttc gttaattttt agaaattttt gttaaatata
    38881 ttatttttag ttaaatacag tctgttttta aacaatatat taatttttat agtatctaca
    38941 ttttaattta aacataaaaa ataagtctaa cctctataaa gtagaatgtt agacttattt
    39001 ttataagtat ttaattatac aaccaaatca ttttttttct tttcttcaag tgctttttta
    39061 agcaccatat ccttaacctt cataagtcta gtagtacttg ctctctcatt ttcttcaagc
    39121 ttcatagcta tatacttaat agtctcaatg taatttggta tcattacatt ttcaagagca
    39181 ttaacacgtc tacgtgtctt ttctatctct tgtgcaagaa gctgtgcact tttttcactt
    39241 tctgcaagtc ttaaaagtgg ttgcatagca tctgagaaag cttccatagc agaatctaat
    39301 tcacctgatg taaaagcaag accataagga taaatatcac tttgattgtt ttcagtttta
    39361 aagtcaaaca caggaacatc aacactcatt atatttctcg tagaaacatc tactgaaact
    39421 gattgctttg gcatcataag tgctgaaccc agatactcct gactcataac tgctcttgcg
    39481 attataaagt ttttataagc agtatcaaga gcattttctg cttcttcacg caatcgctta
    39541 ttttctctta caatctcaag aaattgtttc ataagttcat caagtttatc tttaagaagc
    39601 ttatgacctc ttgtagcagt tttaaggagt ttcttaagtc tagtcatttc catacgtgtt
    39661 ggatttatat ttaatctagc cacagatatc actccttagg catatatttt tcaaaatatt
    39721 catcacggat acgtttaagt tcactttttg gtaacatact aagaagcttc catccgattt
    39781 ctaatgtctg ctcaatagtt ctatcagttc taaatccttg tgaaacatat tctttctcaa
    39841 attcatctgc aaacttagca tacattaaat caatctcaga aagtgcagat tcaccaagta
    39901 tagccataag ttcttttgca tccttacctc ttgaataagc tgcaaaaagc tggttcattg
    39961 tatctgcatg gtcttcacga gtcttacctt taccaatacc tttatctttt agacgagaaa
    40021 gtgatggtaa aacatctata ggaggctgta tatctttttt ataaagttca cggctaagta
    40081 taatttgccc ttctgtaatg tatcctgtaa ggtcaggaat tggatgtgtc ttatcatcct
    40141 caggcattgt aagtattgga atcatagtta tagacccttc atgccctttc atttttcctg
    40201 ctctttcata catagtagca aggtcagtat aaagatatcc tggatatcca cgacgtcctg
    40261 gaacttcttt tttagctgct gaaacttcac gcaatgcctc tgcatagttt gtaatatccg
    40321 ttattataac aagtacatgc atatcttgct cgaaagcaag gtattctgct gctgtaagtg
    40381 ccattctagg tgtagataca cgctctacag ctgggtcatt tgcaaggttt acaaacacta
    40441 cagaacggtc gattgcacca gttgctttaa aatcgcttat aaaaaattca gcatcttcaa
    40501 aagttatacc aactgcagca aatacaacgg cgaacttaga atcagttcca cgtacttttg
    40561 cctgtcttgc aatctgaact gcaagattgg catgtggcaa accagaccct gagaatatag
    40621 gaagtttttg ccctctaacc aatgtattaa gtccatctat agcagagact ccagtttgta
    40681 taaactcggc cggataatca cgtgctgctg gatttattgg agctccattt atatcaagtc
    40741 ttttatctgg tataatttct ggacctccat caataggtct acccattcca ctaaaaacac
    40801 gacctaatat atctggtgaa actccaaagt caagactctt tcctaaaaaa cgaactttac
    40861 tttcagcaag gtttatacct gttgagcttt cgaaaagctg aacaagtgca gtatctccat
    40921 ttacctctag aactttgcaa cgacgaattt ctccatttga aagttcaatt tctccaagtt
    40981 catcaaattt tacaccttct acgccgttta ttaacataag aggaccggct acctcttgta
    41041 ttgacttata ttcctttatc atagttcttc agcccccctc tttttaataa ggtctgcaag
    41101 ctcgttgtct atttcatctt caattgaatc gtaagctgca tctatatcgc cttcttctac
    41161 atacttagca cgaccaatct tttcaagaac tgaaagcttg ataatatcgt ttatagtaac
    41221 attttgcttg atagcatctt gtgctgactt atagaatttt aatataagtc ccatcattct
    41281 atattgttta tgaagtgatg tataagtatc aacttcatgg aaagagtttt gatgaaggaa
    41341 gtcttctctt atagaacgag taacctctag tatcaatcta tctccatctg aaagtgaatc
    41401 aaccccaacc aattgaacta tttcttgaag ttcagattca acttgtaaaa gtttcatagc
    41461 ttgagctctt tgagctgaga aattatcatt tacattttta tctgcccata catcaacttt
    41521 ttcctgatag agtgaataac tagtcaacca gttaattgct gggaaatgac gtttatatgc
    41581 taaagatgca tcaagacccc agaatacttt tactatacga agcgttgctt ggctgacagg
    41641 ttctgatgta tcaccaccag gaggagaaac tgcaccaatt gctgtaagtg ttccttctct
    41701 ttcatccata cctagacaat ttacctttcc tgctctctca tagaattgag caagacgtga
    41761 acctaagtat gcaggataac cttcttcacc aggcatctcc tctaaacgac cactcatctc
    41821 tctaagagcc tcagcccatc ttgatgtaga gtctgccata agtgcaacac tatatcccat
    41881 atctctaaaa tattcagcta ttgtaatacc agtgtatata gaagcttcac gtgcagcaac
    41941 cggcatatct gatgtgtttg ctataagcac agttctttgc ataagtgact cgcctgttct
    42001 tgggtctttt aattcaggaa attcaagaag aacatctgtc atttcatttc cacgctcgcc
    42061 acagcctata tatacaacta tatctgcatc tgcccattta gcaagctgat gctgtgtaac
    42121 agtttttcca cttccaaaag gtcccggtat agctgctacc ccaccttttg tgattgggaa
    42181 aaatgtatct ataactcttt gacctgtaac aagaagtgaa tctggtgttt tcttttctct
    42241 atatggtctt tcttttctaa ctggccattt ttgcatcata gttacaggta tatcattacc
    42301 tttttcatct gtaataacac aaactgtatc ttcaacagta aattcaccag aatatatttc
    42361 ttttatagta cccttaactc cataaggaac cataatttta cattctacta ctgctgtttc
    42421 ttgaacgcca ccaattatat ctcctgctac aaccttatca ccaacagatt ttttaggttt
    42481 aaattcccac ttagttgttc tatcaagtga agatacttca acacctttag ttatattagt
    42541 acctgaaata tcatacattt tttcaagtgg acgttgaata ccatcataaa tagttgaaat
    42601 aagacctggt ccaagttcaa cactcatagg cattccaaga gaaactactt cttcccctgg
    42661 tcctagaccg gaagtttcct cataaacctg tatagaagct ttatctccat gcatttctat
    42721 aatctcgcct ataagatgtt tatctgaaac tcttacgacg tcaaacatat tcgcatctct
    42781 catgccctcc gctacaacaa gcggtcctga cacctttaca attgtgcctg ttttcatatt
    42841 ctctcacctg caatcatata ctttatcaaa atttaaatat tccctcctat attttattag
    42901 cacatatatt tagcaccaat aaaatttgag attgtctttt attcaacttt catctattct
    42961 ttaaataata tatctgcacc tatagcacgt ttactagatt ctcttacttc attcatacct
    43021 aatcccatac ttcctccaat acctggaata actataatgg caggaagttg gtaatctttg
    43081 tattttgcta ttgattcttt tgcaagcaat gatgcctgtt ctgtaatata aattatcgca
    43141 tattcatctt caaccaactt atgtatactt tttttaattt cgtcactatc ataggctggg
    43201 aagatatcta ttccaagagc taaaaatccc atgatgctat ctttatctcc aactactcct
    43261 accttataca taagcatccc ttagcctttc ttttatagta tctgcgtcta tattattaag
    43321 cttactagtc aaaataattc taattgtttt tatctctgat tctttagcat ataaatatgc
    43381 aattagtggt tctaatgtaa gaggcttaaa ttttgctcct ctaacatatt ccataagata
    43441 gtcatcacaa agtttttcaa agacagtaaa accactatcc atgccatttt tacagacatc
    43501 agagtaagaa gtagatttaa aacatgatgc tggagtatct gaggaaaaag cttctaagaa
    43561 tttttcctta tcaagtgagc cacctgaaac aaacacattc ataaacatat caaaatcttt
    43621 cttcatattt ttgactctta taaaactctt tagatttgtt aaatcacaaa ctttcataac
    43681 ataatctatt acaaatttgt tttggctttc tttagcagtt tctttcatac tggtaaaggt
    43741 tgctttatct agaacaatat caaccatctg gccattttgt gtcttattat aaacatcaaa
    43801 tgcctctaat atagctgatg acattatctt aggtaaatca ctaaaactac gatttgctaa
    43861 agattctcta agcttaataa gaggaattgt acctccatca attaaatatt tctcaccatc
    43921 tacaccagaa atttcttctt taatcagcac ttttaaattg tgatagtcat ttttataaag
    43981 aaatacatca acaaattttt cttcaggagc tagactttta agaatttcat atgtctttga
    44041 taattcttga gtcaaaatat tctcaaaatt atgtatatta ttatttgatg tatcagcata
    44101 acctgcctca gctataattc ttaaagagtc ttcagggctt ttggcttctg ccatttgaat
    44161 aaacatttgt ctgttaagaa gctgtttttc aagcactctt attcgtgcaa cggcataggg
    44221 ataagttttt tcttctgcca ttttttaacc cctccttaat taaacaaaat ttctgctgca
    44281 atttgttcaa tatattcatg ctctactgca attatggctt caaatgaata attgtactca
    44341 atgtcacctt ttttaactat gaaacctcca gtaatatctc tagtttcatc agacacttta
    44401 atgccttttt ttgatagcac atcttttaat gtttttttat cttttttaga taatactatc
    44461 tctgaatcat ctgtacaatt tgatttttca atcatgttta gtataatctc ttcatattca
    44521 ttatctttaa gatttaaaag tttgttttta gcttctaaaa ttacctcttc taaaatctct
    44581 tgtttttttg ataatatttg ctttttagcc tccatataag ctcctgatat ttccttagaa
    44641 gccgcttttt ctgcttctgc ctctgcaagc tttgtatagg aagccatttc cttttcagct
    44701 ttttcattag ctgaatttac ttttaaatct gcttcacttt tagctttatc caatatttct
    44761 tgtgcttctt gtttggcatc agctataatt ctgtcaatca ttttttgttc attacccata
    44821 ggagtgcttc ctccttttca ttttttttac ttatgaatta taatttataa ttaaccaatt
    44881 gcaattccat tgtaagctaa gaaagaaaca agtagtccta aaagagcata tgtttcaact
    44941 attgctgcaa atgttatagc tttaccaagt tcttcaggtc tttttgcaac tattccaact
    45001 cctgctgctg ctgctttacc ttgtgctata cctgatacta aaccaactaa accaattgga
    45061 agtcctgcca taagaagttg taaaccttga gctgatgttg gtattgcatc tccaccaata
    45121 atccctgctt ttgataaaat aaggaaagct atgataaatc catacagacc ttgtgtacca
    45181 ggaagaagtt gtaaaactag aagttgaccg aatttactag ggtcttctgt tacaacacct
    45241 gctgcggctt gacctgctat tgaagtacct ttagcagaac ccatacctgc aaataatgct
    45301 gctatagcag ctcctgatat tgccaaaaat tgaccatttc ctaacgcgtt taaaaattcc
    45361 atatttacat ctccttttta ttaatgcttg tatattttgt tttatactta aacggaacaa
    45421 aaggaactcc tccgccttca taaaacttat tgaaaaattc tacatactgt aacctacttg
    45481 tatgaacata tgcacctagt gcatttataa gaaggtttat tgtgtgtcct acaacaccaa
    45541 ttacaactgc aagtatcgga ccagctatag cccctaaaag gttgataact tgtgctataa
    45601 ctcctgttgt taaacaaagc gccataattc ttgtataaga taatatatca gcaaaataac
    45661 ttgttatacc atagagactt gaaaatcctt taaaaagctt aacaggaata cctttaaagc
    45721 ttcttccttg agtaagtaca agacctatag cacctataat tgctaaatat tttcctactt
    45781 ctgaaaatac acctatgtcc cctgcaacaa aaggtattat aagtagacat acacctgtta
    45841 tacacaaata ccaaaaacct atgtcaaaga ttgcatctat tacctttcca cttcttatta
    45901 atgtatatgc ttttattccc aaacctacat atatatggat aataccgaac agtaacgaca
    45961 ttcccataag tgtcataaca tcctctaaag gattaattaa tgcttttata ggaattaaat
    46021 cccccaaaat acccccgaat ataagacccc atacagtagt tgaaactcca cagataccaa
    46081 taagtttaat taaatttcct tcaccttttt gtatctttaa tttatataca acaaatccac
    46141 aaactactgc aattattaac ccatatgcgg cgtcacttag catcattcca aagaatataa
    46201 caaagaagaa agttaaaatt gtatttgggt caatatcttt tgtacttgga tagctgtaca
    46261 tgttagttac actttcaaaa ggtgttacta atttattgtt ttcaagcaga attgggaagc
    46321 cttcttcttt acttccttct tcagtctgaa cacaacaatc aaacttttct gtaatttctt
    46381 taattaattc atctgccctt ttgcttggta accaaccatt aagacagaat gttgtttttg
    46441 ttttgacaag tctctctata atcttttttt ggtctctttc tatagtgtaa aaatcatata
    46501 ggttttcaag catgtgtata ctatctgcat gtcttttaat ttcctctgtt ttgtccttag
    46561 aaacgttttc ctgtctaatt atcatttcat tgtatttctt gatagcttgt aacggtgtac
    46621 cttcttcatt tggtaatgaa accactgaaa aattaaattc ttttaaaact tctaaaacat
    46681 catcaaaagt ttcttttaaa gtaattaaat aaacatagtt cattaacttg tcagaattga
    46741 caatcccaat aacactttct ggtatttcat cttctaattt tactcttaaa gcatcaatgt
    46801 ttgcgcttat gggcacagtt ccaagtatag tttttgtata cttagtttcc atattttcaa
    46861 taggaatatc aaagttcacc catggttcta acatactttt attgttgtta agtaagttta
    46921 tgttagacct tattgtacct atctccttgt gcaaattgtt taactccaga gcttcagcat
    46981 aaacttgccc ttctttttca ctatcaattt tgttaaactc tctttttggt gcaagtaggg
    47041 attttttttg tttcactagt gaagaaacaa tattaattgc ttggtttaca tgagacattt
    47101 tctgctctag cattataact tcagattctt cactatcttt tacaagataa ttttttaatt
    47161 cctcctcctc agtaagaaag gcactatcat caatctgaac aaacccacgg ttcataagta
    47221 gctttaaaat ctggcttttt tgagcttcta agccaacaat agatattttg ttcatattaa
    47281 ctattgccat attatctcac tacctttcca ataaccgctt caatagcctt actttttctt
    47341 tcttcagcag ttttttcaat tttcagacat tcacttttag cattagctaa gattttatca
    47401 acctctgctc tactgtcggt gactgccttc tcaataagag tttcttccat aagctttgct
    47461 tgttttttta catcagcaaa tatgctttca catttgttta cagcatcagc tctgatagac
    47521 tctgcatcag taacagcttt tactttgata ctgtctgctg tctgctctgc ctcaacaatg
    47581 ctttttatta tttcaaaagc catccaaatc acctctctta cctttttact atgtatataa
    47641 ccaattagat ttttttattc taattagatt actacctcat tgttaaagta taaacagcat
    47701 ttttatataa taattaatat ttttcaacgt ttatttctta ataaaacata ttttagaata
    47761 gtacttgcct agatttacat ccttttttga cttactttta gactatgaca tgagtatggt
    47821 ttttaaatac cattattttc aataattata atttaaagcg atatagtttg tcaatagcgt
    47881 tattttgttt taaaattaac gttatgtcga cattttaaat ttttatttta ttttttttat
    47941 aattttaatt taatttatta catataaaag ttttttatat tctttttcaa cctctaacca
    48001 ttgaagttta ttgttttttt tgaattttta gaaatatctc gcactatttt atctaacttt
    48061 tataaatcat caagctatat cgatttttgt caccttattt ttaatctaat ttttttatat
    48121 ctagttagaa ataaatcaat atttttaaag attgataaaa cttacaccta gttctaatag
    48181 ttttatttta aggcaatgta ttaatcttaa gtagataata tcattagtta ctctatatat
    48241 tcgacaacat tataaaaaaa tcctcgtacc aatcactcca atcttcaata ggatatgctg
    48301 ctcctccatt tacacttttc agtagattgg atgtatattc ttctcccata gaaaaaagtt
    48361 cccctactgt catatcataa cctctagatg taacaacctt acaaaatgaa tccataacat
    48421 cacgttgttc tcttgttttt ataagttcct ttctaaattc atcatcattc ttagcttctt
    48481 ctaaaatttt agttaatttt tcattcatta tgtatttctc cttttaagta gtctttctgt
    48541 aaacttcaat aagctttata taaaaaggaa gccatcctaa ataaactagg atgacttcgc
    48601 ttaactgtta gcaacacatt tactagtata aaataattgg tgtacctggt tctatatttt
    48661 catatatttt ctttgcctta gaaaaaggtg tatttacaca tccatgtgaa cctgaagact
    48721 tatatatact tccaccaaaa gaatttctcc aagtagcatc atgaatgcct atgttcccat
    48781 taaatggaag ccaataactt acaggtgaac tgtaaccttg accagttaat gaaacatttc
    48841 tagccttata attaagagga tatatacctg atggtgtagc aaatcctcta gctaaatcac
    48901 ctgttacaac gtctgttgat acaagcattt ctccattttt aaagaaccac atatgttggc
    48961 cagaaagact aatctctaca tatgtatttc caatatcatc tttattacga gatacagctt
    49021 tttgtatgta aataggttct cttgtaacat ttttaccagc ttttacaaca tcaactaatg
    49081 cttcagcttc tttcttttta tctatcaacc aaccataaat tccacctgaa attgtaatat
    49141 ctttacctgt ggcagtttta aatggtcttg agtcaccaaa agtactgtat tttttactta
    49201 aacctcttgt atattctacc atagcatcat tatcaaaagt tatgtcataa ttcttttctg
    49261 agtctacttt taaccattta ttaatttcag aaccatctaa agtttcctta cggtcactaa
    49321 aatcatatgt aacgactaca ccaacatact tatctaatgt cttaatagct tgtttcaact
    49381 tttctgaatc ctttgtgttg tttggtgctt cataaacttt actttcatcc agagaaatag
    49441 aagtattccc agaattaatt gagttttgta ctaacttata aaaatcatct tttttcactt
    49501 tgtttccaag tacctctggt actataacat aagcgttttt agtgctatca tacttaggat
    49561 aagcactctt aggttctaca acatttttac tatcaaaaca agataaagca ttaaaccttt
    49621 tttctagtaa atctttatta tatgaaagag ttatcatatt attatactct ttagatttaa
    49681 atattccacc aatccatcca atagcgctct gactatcttt tatcttttga actttatcat
    49741 ctggtgtaaa ttttaaatca atatcttttg cttttatttg ttctttctta ttgtttcttt
    49801 cctctaagtt taaagtatag gaattagtac tagatgctaa caaatcattt acttgctcaa
    49861 ctgtttttcc tgaagccttt acgccattaa tagtcgttcc aaagtaaaaa tgatttctaa
    49921 aaaagattgc cattcctata taagctacaa acaaaacacc tactactata gctatcttaa
    49981 attttagatt tttttgtcca ctttcttgtt ttacctcttt tccatcaatc ataattaaat
    50041 ccttccttac attgtaatta cctatgtacc ttattggttt ttaaacaaaa ccatgtatgc
    50101 cttattttat atacccaaat tactgtatca ttatttatat ttttaaatat atagttattt
    50161 ctataaaacc attttatctt ttagataaaa tgattattat aaatcccccc tttcaaaatc
    50221 ccttaaacac aatctctgct atattaatat attaccatac ttttaataat cgtgggacta
    50281 tctatctatt tttgtaacac taaactacta ttttaaaaaa gaaactaatt ttgtgttagt
    50341 ttctttatct taacttattt ttatattgaa ttttagctta acataacgtt gttatataga
    50401 ttctatttaa ttattttagt ttctatatct tttccatctg catattcttt aagtaattcc
    50461 ttagcatatc caaaatattc ttcaatatca gattcttgac taaaagaaaa tatacaaatt
    50521 aatatttcat ttgcatcttc tgttatcatt gctctttcag aatcactagt tggactccca
    50581 aatttaccca ctgaatcaga caatatagga agattttcta tatttattaa ttctttccca
    50641 atacccttat actgttctcc ttcatctcct acagtaaaac atactggtga atgtaatttg
    50701 cttacattat atgtacctac tggatataat gattttattg atattaaatt gtttatttct
    50761 actatattat ttattgtata aagacccttc ccttgaataa ttcttctaac caatgcttct
    50821 gaagatgttc tatatctact tggatttttt ccaagctctt tataagcatt tcttgcatct
    50881 ttaattttat ctagtttcgt aatgtcttca acttttagac tttgatttat agtatcacaa
    50941 aactcattta tttcattaat taatttttct gagcttttat ttactgctac ttttgcttga
    51001 atgcaaccca gcttagcatt aggccatctt tccttaagtt tgtcatcaat acttatatct
    51061 atcatactta aacaactcct atacactaaa tttttattaa agtttttatc atctatacat
    51121 tatagtacta ttcttttgtt aataacatgt ttattagcaa aataaattta tttttaatat
    51181 aatttttata aaatacaatt attttttgct ttatattaga caatacattg ataaaataaa
    51241 aaaacactaa ttatttagca atgatttatt aaaatcaagc attcataata gtgctttata
    51301 taaaaatcaa attttgtttt aaagtaaatc taaaaaattc ttagctggca ttggtcgtgc
    51361 aaaataaaat ccttgaacta aatcacatcc aaattgtttc attaactcag cttgttcttt
    51421 actctcaaca ccttctataa ttacaactaa atctagtgat tttgctagag acactattga
    51481 ttttgctata ttgcgacttc tctcatcatg ttcaatgcca ccaaaaaaag acttatctag
    51541 cttcaatata tcaattggca tatctcttaa acaactaata gaagaatatc ctgtaccaaa
    51601 gtcatccata gaaatagaaa atccatacgt cctcaactca tttaaaacat tctttaaaat
    51661 atttaactca ttaatagcca cagtttctgt tatctctatt tcaatgaaat ctgaacttaa
    51721 attatacatt tttattgttt tattgataaa ttttacaata tctggttgat acaactcgac
    51781 cctagataaa tttactgaaa ctggaactat ctttttacct tgctttttcc attcatataa
    51841 atcattgcaa accctttcaa atacaaatct cccaattttt aaaatcaact tacttttctc
    51901 tgctattgga ataaatactg cgggacttat aaaaccatgt tctggatgat tccatcttat
    51961 tagtgcttca ctccctatca tttctccagt tacaagtgaa aacttaggtt gataatacac
    52021 ttcaaattga ttttttacca atgctatttt tatatcgtct aaaatcattg attcctcact
    52081 aagcttattc ctaacatctt cattataaat agcataattt atatttttac ctttagcaac
    52141 agacctagcc atatttgctt tatttacagc cttttgaata tctacttcac cttcttcaac
    52201 aaaataaata cccacacaca gagaaatttc aaagactata ctcaaattac tttcaatatt
    52261 acttcgtata ttatcaagtt ttcttataat tttatttctt ccatttttat aatcacagaa
    52321 aattgcaaaa taatcacttg aaagtcgtgc atatacctca ccttctgtta attcttcttt
    52381 taaagctttt gctattgtaa ttaataaatt gtcaccaaat tcatatccaa aagtatcatt
    52441 aatcatctta aagttattaa tatcaaaata tactataata taatttaaag atggtttatc
    52501 taaaagatac ttactacatt ctctataaaa tttattttga tttcctatat atgtaacttt
    52561 atcttcataa gccaaactta atatttcttt ttcattagat ttttttatat aaattatata
    52621 aaaaataatt gcaacaaata ttaatacaat agataacact gcatatacag ctctgttgat
    52681 tatttgtttt gaattactaa atacaacagt ttctggaatt gaaaaaatta aattccagtc
    52741 ttttatataa ttattttcac tattcccatt atccatagtg gcatacccta aatatcttct
    52801 ttcttccaac atatttaata cagttgcacc tgttttattt tcttgcaatt ctttttttaa
    52861 tctatttact tcttgaatat cattatcctg ttctaacaat ttataaatat tttttgatag
    52921 gctatcccct tgtggatgaa ggataattgt accatcattt tttacaacat aagatatact
    52981 attattttca taaaaagata tattaataag tttcatcaga gtattgatat tgtaagtaca
    53041 atataaaact ccaactactt tatcattatt taataatggt actgaaatag caatcttctt
    53101 tatagactta tttacttcat caacatatgg acttgaaaca tacttcttac ctttaattga
    53161 gtttttgaaa aattctctat ctttcactga aaacttatca cctttggtat tttcagcatt
    53221 accatttata tcaactattg ccatagtgta aaatttttga ttttccattt catctaaaag
    53281 atttttcatt ttttcctcat taggtatatc tggatttgaa gcataatagt tagaaaaaat
    53341 atttaaaaca tctaaatctt tttcaatttg tctttgtgct agttgagagc cttgtactgc
    53401 aacttctttt atatgtattt ttgcctctga cataagaaga tgttttgtat cttctatata
    53461 gatatacccc attgtcaaaa ataatgatgt taaaaaaaga attgtaatta atgtaaaact
    53521 tttatttctc cctgtcattc gactcctccc ctaattagtc aatacttcat atagataata
    53581 tatccttttt ataccaattc atcatttata ttgtatataa ctattcttaa atgttacaca
    53641 taaaatcaac ataataacca taaaaacatt cttttattat tataataaca ctatttaatt
    53701 gaattttaaa ttaaagaata aaaactatcc attttctcaa acaaaaaaac tcttatacaa
    53761 taaaactttg ttttatgtat aagagttttt attttatcta ttttatagca aacttatctt
    53821 ccataatatg aatttaaata catatctttt atttcattca taagtggata tcttggattt
    53881 gctcctgtac attggtcatc aaatgcttga attaccattt catctattga atccataaac
    53941 ttagattctt caacgccaaa ctcttttata gatttaggaa taccaacttt aagttttaaa
    54001 tcttcaatag ctaccaataa actttggaat ttctcttcat cagaatttcc ttttaatcct
    54061 aaaaatgaag ctatttcagc atatctcttt aatgcttctg gatatttata ttgagagaat
    54121 gctcccattt tctttggtgc atctgaagaa ttaaatttca taacttctgt tatcaaaaga
    54181 gcatttgcaa caccatgtgg tacatggtgg aaagcaccta atttatgagc catagagtga
    54241 caaacaccca aaaatgcatt tgcaaaagcc atacctgcca ttgtagaagc atttgccatt
    54301 ttttctctag cttctgggtc ttttgcacca ttatcatatg cacgtggaag atattcaaat
    54361 atactcttaa tagcttgtaa agctaatcca tttgtataat cggttgcaag catagataca
    54421 tatgcttcta atgcatgagt taaagcatct ataccagaag cagatgttaa gctttttggc
    54481 atattcatca tcatatcaga atcaactata gccatatttg gcatcagttc ataatctgct
    54541 aatggatatt ttactcctgt atcttggtca gttataacag caaatggtgt tacttctgaa
    54601 ccagtacctg ctgatgttgg tatagctaca aaattagctt tttcacccat tttagggaat
    54661 gtatataccc tttttcttat atccataaat ctcatagcta ggtcttggaa atctacatct
    54721 ggatgctcat acaatgtcca cattatcttt cctgcatcca ttgcacttcc tccacctata
    54781 gatattatta catctggatt aaattctctc atagctttag cgcctatctt agcacattcc
    54841 aaagttgggt ctggttctac ttcaaagaat gtagtgtact taatattcat ttcatctaat
    54901 aaatcagtaa ctgctttagt atatccatta ttgtaaagga atgtatctgt tacaataaaa
    54961 gctctcttct tattcattac atctttaagt tcttttaatg ctactcctaa acaacctttt
    55021 ttgaaataaa ctttttctgg tgctctaaac caaagcatat tttctctcct ctcagctact
    55081 gttttaatat taagtaaatg cttaactcct acattttcag atactgaatt tccaccccaa
    55141 gacccacatc ctaaagtaag agaaggagct agtttaaaat tatataagtc ccctatacca
    55201 ccttgagaag aaggagtatt aattaaaatt ctacaagttt tcatggctga agtaaactta
    55261 tcaagttttt ctctttgatt tacatcatca atatataatg aagatgtatg cccaaatcca
    55321 ccatcttcta ctaatttttc tgcttttcta actgcatcat caaaatcact tgccttgtac
    55381 attgcaagta ctggtgaaag tttctcatga gcaaaagctt cttctatttc aactgattct
    55441 acttcaccta ttaaaacctt agtatctact gggacttcaa agcctgcaag ttttgctata
    55501 gtacaagctg tttgcccaac tattttagaa tttaatcctc catttactaa aattatattt
    55561 cttatttttt ctgtttcatc tttatctaat aaataagcac cacgctcttt aaattctttt
    55621 ttgacttcat tgtatatatt ttctaatact ataaccgatt gttcagaagc acatatcata
    55681 ccattgtcaa aagtcttaga tacaagaatt gagtttacag cagtttttat atctgcacta
    55741 tcatcaatta ttgctggagt attacctgca ccaactccaa tagctggttt tccagatgaa
    55801 tatgctgatt taaccattcc aggacctcct gtagcaaggg tcaaatccac ttctttcata
    55861 acagttgtag ttaattctaa agatggctca tctatccatc ctattatacc ttcaggagct
    55921 ccagctaaaa cagcagcctc aagaacaact tttgcagcct ctatagtaga attttttgct
    55981 cttggatgtg gagagaaaat tattccattt ctagttttta aagctaaaag tgctttaaag
    56041 atagcagtag aagttggatt agttgtaggt acaactgctg caataacacc tattggttca
    56101 gcaattttag tcataccaaa tgcttcgtct ttctctataa caccacaagt tttagtttct
    56161 ttatatgcat tatatatata ctctgaggca taatgattct ttataacctt atcttcagca
    56221 attcccatac cagtttcttt ttgtgccatt atagctagag gaactctttg cttattagct
    56281 gccaaagatg ctgctaaaaa aattttatct acctgctctt gactatatgt agcaaatatt
    56341 ttttgagctt ctctgatttt tgctagtttt ttaactaagc tttcaacgtt atttacagta
    56401 tctaaaccaa ctgacataac ttcattttct tttttatttt ctattattac ttgttcttta
    56461 tgagccattt taacaacctc ctgagatagt tttttattta ttatgttaat ttattaacat
    56521 tatagcattt acttttccta ttttcaagtt atttttttaa cattttattt tttatctaat
    56581 ataacatcat ttctactaat aatatctagc tttacaaata cttaatttaa ctagatatca
    56641 ctttattttt tacgcaaatc actaatgaaa tcatctaaaa acttaaggat aacgttattt
    56701 ttttaacgtt tatatttttg tattttttgc aaataaaact ttataaaaag aatttttaat
    56761 atgatactct atcaagttta aaaataaaaa tccttgaaaa tgaaaattaa aacttaatta
    56821 acaagttaaa gtaaatatag ttaaatgtac aaacatttac aacatatagt ataattaaaa
    56881 aaatatatat tcatgatttt tatataatat gaaataaaat atatttaata ttatatagat
    56941 aaatttgaag tttataagaa ggaggataag catggaaaaa ttaaaaacaa ttagcgtttt
    57001 ttctttgatt ataagtgtaa ttttaacgat tggaggtatt ggaatagtaa cttattatgt
    57061 agataatttg tttattagag gactatcagt atttgtttta attatgtcct cttcttttgt
    57121 atcaacaaca gttagattga tatttgaaga gtcaaaacga tataaattct aatttaaaaa
    57181 ttggattaag taaataattt taatatagca aaaattacgg agattagctc aaaatagact
    57241 ttttagtcca tgaggcattt ttttgtatga aattaaatat attttcatca tttcaacaag
    57301 aaaaagaggc ttctctctat tttgagacag cctctttgct agtattgtat ctaatacata
    57361 atattataag aaccaattct attgatgtgg actttttata acaggctgta gctgtttgtt
    57421 ctctagagca agttctatag ttggaattat tagctcagta tgtgcaatca tagaattagg
    57481 ctttgctttg caattatctt gaccaaaagg tacaaaatat atatttttag aattaaggag
    57541 aatgccaata tttttgaaat taaagcttag agcatcattc gttgatattg atattactag
    57601 tggtttatca ttacgaagat gaccttttgc tgccattaat actggtgaat ctgtaatacc
    57661 atttgctaat tttgcagcag tatttccagt acaaggagct attattataa tatcagcaat
    57721 tcctttaggt ccaaatggct ctgcctcttc aatcgtaact ataggtttat ttcctgtaag
    57781 ttcatatgct tttttcataa actcttcaga atttccaaat ctacaatcga tattttgtga
    57841 tacatctgaa aatatagtgt gtacattagc tccttcttta accaaattct ctaactctat
    57901 aaatatttta tcataagtac aaaacgaacc agtaaatcct actccaattg taagtccttc
    57961 aagtctcatt tctaatccct tctttcattt ataatatttt ctatagcgtt aacaagaatt
    58021 tcaccagatg tctttggtga atatttacca ggaagcccaa ggcaaagagt cgcattaatc
    58081 cctgcttctt taacaacaga ataatctact cctccaggag aagaagctat atctataata
    58141 gttacttctt gtgatacttt ttctaacctt tctttcgtta aaatacactc tggtatggtg
    58201 ttaaaaatat aatcaaattg taaaagtttt tcatcaagtt gacttaaaga aatactggaa
    58261 tatccaaatg cattagccgt acttcttgca tcattatttc ttgcgccaac acatacattt
    58321 gcatccaaag cgtataattt ttttgcaagt atttgtgcac atcttccata acctaatact
    58381 aaacagttac tttgatgtat attgattata cttcttttaa tagcttctgc tatagcccct
    58441 tcagctgtag caactgcatt taaaatagca acatcattca ttttcataaa atcataaatc
    58501 tgaatagagt gttgctcgca aaatttacaa actttagttg gtatgttacc tccaaataat
    58561 atttgattag gagataaatt aattagcaag ttatcaatag tagcatcaag acaatcatcc
    58621 gtattttcaa tattaacttg attctttgta aatggaattg gacataaaat aatggaactt
    58681 ttggatagtg cttcagctag tgaagttgat tttccaacga ttccatttat aatttcattt
    58741 gatatcccat aagtgactac agaataccct ttattaatta gtttttttac catgtacact
    58801 tgtcgcaggt ctcccccaat aacagttata tcgtattgat ttgacatatt gtaagcctcc
    58861 tttactatat acaatatgtt tttatcaata cattggttca ctaaaattac taaatattat
    58921 acttataaca tttttaataa tgctaatcta tctatctttt cagaagaggt atatggaaat
    58981 gaatttaaaa agataatttt ctttgggatt tcatttttca ttagattatc tttcatttta
    59041 atcaacaaat ctttcttcgt aattttgtct atagttatta caaataatac aatttgattt
    59101 cctcttatag tgtctgagta cggcaataca attgcatctt caatttgagg aattttttta
    59161 acttcatttt caatcttagt agaactaact ttaaatcctc caatatttaa aatgtcatca
    59221 cttcttcctt caaatattaa gtatccatca ttatcataat atccaatatc attgacagaa
    59281 taaggtttgg ttgcattata tactgcatat tctgtattca caaaaatttt tccttctcta
    59341 atatatatat ctattcctgg aaatggtctg cctacactta gaggtttttc aattagttca
    59401 tcatagcata aatatgtaat ataatttaat tcacttgccc catagtataa aataatttca
    59461 gactttggca tatatttttt tagatttctt gcagtatctt caaataataa ttgagaccct
    59521 gtaaaaatac ttacaacttt gagtactggt tcttttaagt gtttaaccaa aagttgaagt
    59581 tttgtaggta taaggtatat gttagttaca ttatattgtt ttattatcct tatccatgat
    59641 ttacaattaa gacctgaact tattacaata ctacctcctt catataatac tgacattata
    59701 gaatttagat tccctgtaaa actaagtgtt ccattaacaa acaagattga ttccccagag
    59761 atattaaaaa taccattttg tataggaaaa aatccagccc aactctcata tgttctatat
    59821 aatacctttg gaacacttgt agaacctgaa gataaagctc ccatacaaat acttttatct
    59881 aaataattta taaggttctt atattgatac atatatatat taaatttagg attaaaaata
    59941 gaaaaattaa ctttttttgc tacttcatta ttgttatcat catataaatc aataacgtcc
    60001 atagattcat cagatataat gattgaaata ttatttttta ataaaatatc atctaatact
    60061 tttttagaaa gattataatg acaaataata ggaatatttt ttgaataatt aatagcaaaa
    60121 aaactaatca actgaaacat tatattttta gaatatatta aaattgggat attttcacct
    60181 aaagaaattt gttttcctag ttcctcacta taactcaata tattttcata agtatatttt
    60241 tctccatcta taattaaaaa atttttattt ttatgcctct ctttttttat tcttaaaagt
    60301 tcataatagt tcataatcta caccctttca agcagtaaag ctgaacctag accaccagct
    60361 gcagctatag agcaaattcc atatctaccc ttagtctttt ctaatgcttt taaaagatgt
    60421 aatgcaataa ttgcacctga agcaccatat ggatgtccat acgctaaagc tccaccaaat
    60481 atattatatc tatctattaa ctctggatat tttctttgaa acaacacatc tataactgca
    60541 aatgcttcat taaattcaac agcagataca tctaaataat taagactttc tatctctaaa
    60601 agtttatcca ttgcttgtat cgctgatgtt ggacttaagg ttgcatctgt tcctattgtg
    60661 catgtattaa taatttttgc tttaggcttc ttttttacat gttctaaata cttcttagaa
    60721 catattataa taaatgaagc tccatcatta ataagacatg aatttgctgc atttgtaata
    60781 gtttcttttc ctaaaattga tggcatccta tctaataacc tttgactcat cttatcacgt
    60841 ataccttcat cataggtact attatttata ggagatataa tatcttcaag tattttttcc
    60901 tctcttgctt cttttgctct tttatgactt tcctttaccc aaaaatccaa gtctgctttc
    60961 tcaatttgat ataactctgc tactctttcc gcaccttcga gcatactatt ttggctatta
    61021 tcatcaggag aaaattgagc aactgtataa tttggattat ttgtattata tcgcttatca
    61081 ttcttatgat atgtgcgcat tggttgtaga gaactacttt caaaaccacc tgcaataatt
    61141 aaatcacact gaccagattt aacctttgaa aaagctatgt ctatactcat catagcagaa
    61201 gcacactgca tatccacagt aaaagcaggt acttcatttg acactcctgc tgttaaagtc
    61261 attaatctag ctatattccc tcctgtccca actgcatttc cacatataat ctcatcaatt
    61321 ttatcaattt catacttttt aattaaatct tttaaaacac ttgctcctag taattcaggt
    61381 tgaacaaatt gaaaaattcc attttttaga cctatatgac ttctcaatcc tcctaatata
    61441 aaaacttcct tcatacatta cacccttcct tatattatag atttatcata gatttcttct
    61501 gagtacattg ttcagtttag tgcctaaaaa agatgcaata actgacttta ttgtatctcc
    61561 aattaaaaat ggtactactg ctgcaaaaag tgctgcttct actgtcattt gattaaaata
    61621 gcacataaat actgttccca taaaatatat aataggcatt ccaacaaaaa ttgttattag
    61681 tatatatctc ttaatatcat tatttcgtcc ttttaataaa ctaataatta gtgctgcaaa
    61741 caaaaaccca aaataaaaac cacctgttgg gcttaataac tttccaatac cagatgtacc
    61801 tccaccaaat actggaatcc caattaaacc tatcattata tatacaccaa ctgtactaaa
    61861 tgcttgtctt ggtgttaaaa tcaaagctat taaatttatc attattgttt gtgctgttac
    61921 tcctgctgga gtaaatggca atggtatata tatgtaagat gatatacaca ataatgctac
    61981 acatattgac attttagtta aatcttgaat atttacttta gtatttttca tttttataat
    62041 tcccttcata tgtcttattt catttagttt atatgtacat atacaaagct tctattaaag
    62101 ctttgtttta gtataaaata aagacgtttt tttgtcaact attataagca tttaggttta
    62161 catttaattt ataagtaaac tttatactgt tactttatat tttaatagtc atttataaaa
    62221 ctttgcttaa aaacagc
//
LOCUS       DAMKMI010000006        61977 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMKMI010000006 DAMKMI010000000
VERSION     DAMKMI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 61977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.006"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(145..3480)
                     /locus_tag="PC429_000373"
     CDS             complement(145..3480)
                     /locus_tag="PC429_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF3845897.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(3530..3958)
                     /locus_tag="PC429_000374"
     CDS             complement(3530..3958)
                     /locus_tag="PC429_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845898.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(3948..4328)
                     /locus_tag="PC429_000375"
     CDS             complement(3948..4328)
                     /locus_tag="PC429_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF3845899.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYKSKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(4361..4669)
                     /locus_tag="PC429_000376"
     CDS             complement(4361..4669)
                     /locus_tag="PC429_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845900.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(4714..5874)
                     /locus_tag="PC429_000377"
     CDS             complement(4714..5874)
                     /locus_tag="PC429_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF3845901.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKG"
     gene            complement(5878..7341)
                     /locus_tag="PC429_000378"
     CDS             complement(5878..7341)
                     /locus_tag="PC429_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF3845902.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            complement(7850..8719)
                     /gene="gatY"
                     /locus_tag="PC429_000379"
     CDS             complement(7850..8719)
                     /gene="gatY"
                     /locus_tag="PC429_000379"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF3845903.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(8739..10655)
                     /locus_tag="PC429_000380"
     CDS             complement(8739..10655)
                     /locus_tag="PC429_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF3845904.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGL
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(10658..11578)
                     /locus_tag="PC429_000381"
     CDS             complement(10658..11578)
                     /locus_tag="PC429_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF3845905.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(11680..12432)
                     /locus_tag="PC429_000382"
     CDS             complement(11680..12432)
                     /locus_tag="PC429_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3845906.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(12824..14269)
                     /locus_tag="PC429_000383"
     CDS             complement(12824..14269)
                     /locus_tag="PC429_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF3845907.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKERYKKDSFYWYQNVIRTNGEELREK"
     gene            complement(14291..14644)
                     /locus_tag="PC429_000384"
     CDS             complement(14291..14644)
                     /locus_tag="PC429_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3845908.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(14673..15920)
                     /locus_tag="PC429_000385"
     CDS             complement(14673..15920)
                     /locus_tag="PC429_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3845909.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFTVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(15928..16245)
                     /locus_tag="PC429_000386"
     CDS             complement(15928..16245)
                     /locus_tag="PC429_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3845910.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            16487..18400
                     /locus_tag="PC429_000387"
     CDS             16487..18400
                     /locus_tag="PC429_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3845911.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(18523..19677)
                     /locus_tag="PC429_000388"
     CDS             complement(18523..19677)
                     /locus_tag="PC429_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF3845912.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKDTIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(19937..22552)
                     /gene="mngB"
                     /locus_tag="PC429_000389"
     CDS             complement(19937..22552)
                     /gene="mngB"
                     /locus_tag="PC429_000389"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF3845913.1"
                     /translation="MKKNVHIVPHMHWDREWYFSTEESRILLVNNMEEIMDMLENNHN
                     YPYYVMDGQTAILEDYLAVKPECKERIKKLVQEGRLIIGPWYTQTDEMVVGGESILRN
                     LLYGIKDCDEFGEYMKIGYLPDSFGQSAQMPQILNGFDIKYSMFWRGCSERKGTNKTE
                     FNWKSDDGSSVLVQILPLGYAIGKYLPMNEDELRTRMDKYLPVLDKGATTDHIILPNG
                     HDQMPIQKNIFEVIYKLKECYPERKFFLSRYENIFKELEKNEDIDTIKGEFLDGKYMR
                     VHRSIFSTRMDIKSANARIESKITNILEPLASIAYSLGFEYHHGLIELIWKEIMKNHA
                     HDSIGCCCSDKVHHEIMNRFFLAEEKVDQLITFYKRRIVDAMSCEMALDKLVAFNLMP
                     YDRNEIVSAQVITKMKAFEIFDKDNNKLDFEVVHKEVIDAGLIDRQIVHYGNYDSFMV
                     YTINFKDKIPAMGYKAYMIKEVEHMLEKAYEVCDMVDNDFYTIQVNENGTLKIFDKKL
                     NKTFENVLLMENGGDEGDEYDFSPLPNEKLIFNDNIVANSTIKKNRFNNEIKINYRLK
                     VPKDIQARKNNRADSFIDFDIVINVPNDKAIIDVVFNINNQARDCRVRTYIPTNIASK
                     FSVSDNQFGHIKRSVYDDAMEVWQQEEWSERPDSIYPMLTFVGLSDKEHGVAVLTNST
                     REFEIVGEKFDTIAITLFRSVGFLGKEEMARRPGRPSGIKLPTPDSQMIGDIKIDLAI
                     VTHEKSTLEANVANMAKEYLTKIQTYNKMPYNAMKLNQSEIEVDYSYSLLKETNKDLV
                     LSVVKKAEKQEALVVRFYNSKEETKSANIVFNKNIKTAKWINLNEKELESISIDNNSV
                     NVECLMNQVKTILVK"
     gene            complement(22696..24621)
                     /gene="mngA"
                     /locus_tag="PC429_000390"
     CDS             complement(22696..24621)
                     /gene="mngA"
                     /locus_tag="PC429_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF3845914.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AVLLIITRKQKNQRKIH"
     gene            complement(24751..25476)
                     /locus_tag="PC429_000391"
     CDS             complement(24751..25476)
                     /locus_tag="PC429_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3845915.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEVAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(25739..26509)
                     /locus_tag="PC429_000392"
     CDS             complement(25739..26509)
                     /locus_tag="PC429_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF3845916.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(26511..27938)
                     /locus_tag="PC429_000393"
     CDS             complement(26511..27938)
                     /locus_tag="PC429_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3845917.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            28329..29051
                     /locus_tag="PC429_000394"
     CDS             28329..29051
                     /locus_tag="PC429_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3845918.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            29248..30915
                     /locus_tag="PC429_000395"
     CDS             29248..30915
                     /locus_tag="PC429_000395"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF3845919.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(31011..32330)
                     /locus_tag="PC429_000396"
     CDS             complement(31011..32330)
                     /locus_tag="PC429_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3845920.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(32371..33117)
                     /locus_tag="PC429_000397"
     CDS             complement(32371..33117)
                     /locus_tag="PC429_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF3845921.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(33361..35370)
                     /locus_tag="PC429_000398"
     CDS             complement(33361..35370)
                     /locus_tag="PC429_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3845922.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(35764..37197)
                     /locus_tag="PC429_000399"
     CDS             complement(35764..37197)
                     /locus_tag="PC429_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF3845923.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(37284..39200)
                     /locus_tag="PC429_000400"
     CDS             complement(37284..39200)
                     /locus_tag="PC429_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3845924.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGVFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(39274..40134)
                     /locus_tag="PC429_000401"
     CDS             complement(39274..40134)
                     /locus_tag="PC429_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3845925.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(40485..41672)
                     /locus_tag="PC429_000402"
     CDS             complement(40485..41672)
                     /locus_tag="PC429_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3845926.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(41686..43077)
                     /locus_tag="PC429_000403"
     CDS             complement(41686..43077)
                     /locus_tag="PC429_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF3845927.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(43672..45372)
                     /locus_tag="PC429_000404"
     CDS             complement(43672..45372)
                     /locus_tag="PC429_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3845928.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(45344..46747)
                     /locus_tag="PC429_000405"
     CDS             complement(45344..46747)
                     /locus_tag="PC429_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3845929.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(47011..47502)
                     /locus_tag="PC429_000406"
     CDS             complement(47011..47502)
                     /locus_tag="PC429_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3845930.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(47528..48340)
                     /locus_tag="PC429_000407"
     CDS             complement(47528..48340)
                     /locus_tag="PC429_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3845931.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(48353..49504)
                     /locus_tag="PC429_000408"
     CDS             complement(48353..49504)
                     /locus_tag="PC429_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF3845932.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            49774..50415
                     /locus_tag="PC429_000409"
     CDS             49774..50415
                     /locus_tag="PC429_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF3845933.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            50441..51985
                     /locus_tag="PC429_000410"
     CDS             50441..51985
                     /locus_tag="PC429_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3845934.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(52185..52811)
                     /locus_tag="PC429_000411"
     CDS             complement(52185..52811)
                     /locus_tag="PC429_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3845935.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(52946..53662)
                     /locus_tag="PC429_000412"
     CDS             complement(52946..53662)
                     /locus_tag="PC429_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF3845936.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDKAATKFGLE"
     gene            54088..55302
                     /locus_tag="PC429_000413"
     CDS             54088..55302
                     /locus_tag="PC429_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF3845937.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            55922..57250
                     /locus_tag="PC429_000414"
     CDS             55922..57250
                     /locus_tag="PC429_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845938.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(57378..58568)
                     /locus_tag="PC429_000415"
     CDS             complement(57378..58568)
                     /locus_tag="PC429_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3845939.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSKNGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQSVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(58674..60053)
                     /locus_tag="PC429_000416"
     CDS             complement(58674..60053)
                     /locus_tag="PC429_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3845940.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(60084..61292)
                     /locus_tag="PC429_000417"
     CDS             complement(60084..61292)
                     /locus_tag="PC429_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845941.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
BASE COUNT        20237 a        11099 c         6848 g        23793 t
ORIGIN      
        1 gagatattgc ataattattc aatacctctc tttgtatatt tacattatcc atatctagtt
       61 atctatttca acccaatatt agactaccta ctaataaatt tattctctac atcattaaca
      121 ataattttgc actaaataac atcattagcc tatctctgat gcattttctt taattttatt
      181 tatagtttta atcaatttat ttctctcttc aatatgattt tcaaataaat caattatact
      241 tccaacagtt ctaaatgggt cttccatcaa cactttgtta tcctctataa atccattctt
      301 gactacataa tcgactataa gtttaacaaa atgtatttgc ttagtattaa agctcttatt
      361 atttaaaaac tctgaaaaag cttcatttgc agcttctcta tccaatccca ctagtcttct
      421 cactaattgg cttactggag tatctccaaa ctccttctta taatcatcac aattacctaa
      481 ctgttttaag agtatttcct ccaacgtttc aacatcttgc tttgttatct ttttattatt
      541 tcttaattta aatattgcta attcatcttt atggctattt aaataatact ctaccttctt
      601 tttataattc ttaagattgt ttgcattata tacagaatca tttctatctt ctgctacaat
      661 catatcttca aaacttgtat aatatatctt ttgactagac ttttcaagat acttaataag
      721 ctctcttaat gatatacgta cttcttctac ctcaaatata tctgaatctt cccaaaaatc
      781 ctctgtcatt gctttttcaa tagtatattt ttgttgctga atctgtggta tagtcccaag
      841 ttttgaaagt ttttctgctg tagccattac atgccttata cctttacttg cattattatt
      901 ttgtaagtat gcaagttgta tattatacat aactacatca aatcttttag ccaattcatc
      961 atcactatat gaagttatga gtggtgatat atattctctt atctcatttt gtgctactgc
     1021 tccaatattg ctccaaacat tttcattttt atacttatgt acataactta aattcatcct
     1081 aactatataa ctatcctcat ttaaagcttt tacagaagtt ataagttctt ctaacaattc
     1141 ttttctaagt tctgcatact caccttctat atatttaata tcttgaagtt ctttaaccat
     1201 acttaccttt atattaaata atttttcagt aagtgtttct gttttatttc cctcaaatcc
     1261 tttaggatta aatttaaaaa attcaaaatt attacaaaaa tcaaatatta agaatctttc
     1321 cttatccata tcaacaccta ataaatctgg acataatcta gttcctctac ctatcatttg
     1381 ccaaaactta gtcttacttc tcactttctt gaaaaaaaca agatttagta tttcaggtat
     1441 gtcaattcct gtatcaagca tatctactga aactgctatt tgaggtaatt tattcttatc
     1501 agaaaaatca tctattatag aatctacata atttatacta tagtcaactg cttttacaaa
     1561 gttcccacca tactttggat acagtttatt aaatctttca actattgccc tagcatgctt
     1621 tgtattcttt gcaaaaatta tagtcttacc aatcttttct tctccttcta ttttcaaccc
     1681 ctctgtcatt agcttattta aaactaaatc tattgtgtca gaattaaata gccactcatt
     1741 tactgcatta ttccctattt cgtctccaat actctcatca tcattaaatg tattttcata
     1801 ttcttccttc tcttcatcag aaagctcatc atacttaatt ccatcttcca taatcttagt
     1861 cttaaactca atactagtat aatctactaa atatccatcc tcgactgcct tattatactc
     1921 ataagcatat gtaggtactc cattttccat gtcaaaaact gaataagtat ttttatctat
     1981 ctcatccttt ggtgtagcag taagacccat taagtaagaa tcaaaataat caaagattga
     2041 cttatacttt ttataaatac ttctatgcga ttcatctatt ataattaaat caaaatgacc
     2101 aggtgtaaac aatctctttc catccttaga cttcgtatca tcaattgcat tcatcattgt
     2161 tggatatgta gaaaatatca tcctagcatc ttctgggctg tctttgctat ccaataaatt
     2221 acataacgcc aagttaggta atagcttagt aaaacttttt ttagcttgct ttaccaatgc
     2281 ttttctatct gctagaaata atatattttt aatccaatta tgtcttgaaa gtacatcaac
     2341 tatagatata gcaattcttg tttttcctgt accagtagca cataccaaaa gagttttgcg
     2401 ttgcttattt tcaagagcct cacatacagc cctaatagct tctttttgat aatatctatt
     2461 ggatatttca tcatttattg ttacagaact taaagacttc ttactctttc ttctatctat
     2521 cataagttgt aattctgatt ttttataaaa tccataaact ttcctatctg aatagtcatc
     2581 ccaaatatac atatcaaaac cattagtcaa aaatattacc ggtctttgac tatatctctt
     2641 ttccaaacaa tctgcatata atttagcttg ttgttgacca attttcgggt ctttactcgt
     2701 tcttttcgcc tcaactactg ctaatggctt accattttca ccataaagaa cataatctac
     2761 atatcccaca ccactttcat taggcatacc ttgtacttct atttcttccc atatatcttt
     2821 tccaaattcc caacctgcaa gctttaattc tatatttata tatctttttc ttgtctcaaa
     2881 ttcgcttact tcgtctatct tgaaatcata gtttacagta ttaacttttc tcttttcagt
     2941 tagtgacttt cttaattcct cattttcttt tattacttct tcaagctttt tatcttttga
     3001 acttaatctc tcataaagat ttttaagttc gtctggtctc gtccttttct cttccccatg
     3061 aagtaataaa tcttcattaa agtcttctgc tgtatattca tctgaataac aatagtctat
     3121 ccaactaaca aattgatgca aattgttaag tgaaagtatt gcttctcctc tctctatgtt
     3181 cgcactagta tgtacagata aattacctaa tttaactata tattttaata aaggtaacat
     3241 atcatagtct ataagttcta taaaactatt attatgaatc aacgtactaa gattatcttg
     3301 ataaggcaat actaaatcac tatcattagc atatagccat tttactgcaa gctctaaagc
     3361 tcttcttgtt atagttgcac aagtagatgg gctaactagt atactttttt ctgcttctaa
     3421 gcaagcagta ttaaaactat tgaaattttt cttcttttgt aaaaaatcaa aattacccaa
     3481 ggcttttatc ccccttttgt cccttagtta aaataaatct ctctaaaaac tatttcttac
     3541 agctgtttct tatactaaca aatacttctt cattttctgt aaacaattcg tacatatctt
     3601 caaactgtgg tatatcatat ccatgagaat catcatttct caattttcta agtgtttcta
     3661 taactataga agaattttta ggtaataaat tgtgatactc acaagctaaa aggtagtcat
     3721 aaaagttttt agattttatg tttaaatcaa ttgatttata atacaacatt gcttttgtta
     3781 attctagaaa tgataccata tagcttttaa agccttcttt tatgtatcta gcccataact
     3841 caggcatttc ttccttatca tataagattt tgaagttttg caggtcatac aagcaagaac
     3901 caaaatccct atttacttct tctattgagt tccaagataa tttgtttcta attaccatac
     3961 atgtccctcc aatctaagat atctattaca tcactgtttt caaaatagaa tttatttata
     4021 taatccttaa aatctttaga tttataaata acttcacctt ctaaaatagc ttttttaagt
     4081 gctattgtat aattatctgt aacaattttt aggtctacat caatgtctaa tatatttctt
     4141 agagcttctg tcttagttgc aagttctgta attggtactt ttttgatggg aatccaagct
     4201 atgtctatat cactttctga tgtatgttta tctgttccat aactaccaaa tattatgatt
     4261 gctagagtat tttcttttgg aaatacattt attattgagt ttttaatctc atttggtata
     4321 tttctcattt aatcacctct tgtttattag gtaatattgc ttaattgaat ttatatccat
     4381 aatcttttaa tctatttata catatttctc tttcttcatc agtaaacaac tctttatact
     4441 tttcttttaa tactaatact tctaatgata aatctaatct cttacactcc catagccttg
     4501 taaatccttc agtagctcct tctttattga ttaattcttt agcagctaat aaggctcctt
     4561 tgttatttat cagttgtaaa aatcttgttg gcttatagtt acattgtaaa tcagcttctt
     4621 tatagatatt taccatgtta tggtataaat ctagctctat ttgattcata gattttattc
     4681 ccccatttta ataattgtat tttagttgaa taactatcct ttaaaagctt tttgcattaa
     4741 tgagttaaag ttgtcttcta gttcttttag acttgttttt attcctactt tcaatttgtc
     4801 tacttgatta acaaactctt caaacttatt ttgaagttct attgatggta ctggtattaa
     4861 tattttttga agttctttgc ttctaattcc ttgtgcagag ctaccagttt tatatttatc
     4921 tatttctacc ctaaaagctt ttgtttgcat atattgtgct aaaaaagcat tatttaaaat
     4981 ttttttatct ccctgaattg ttataatcct ttgacccata gcaaactttt ttaaatcctt
     5041 tggtatccta catacatttc caaatgtatg accttctgta acaaaaagta catctccagc
     5101 tactggaaaa cctcttttca tccaagaatt ataggtgttt tcagatatat attcattaat
     5161 atatttaaat acaccattcc ctacagattt tgcatttatt attctaattc cattctctga
     5221 aagaattgga gttttaccac gataatcaat aaaatttgta caatctttca ataatacaaa
     5281 cttttgttcc ttaaaatttc caaacatttc aataaatctt gatttaacta attcatccaa
     5341 tgcttctatt tgctcttttc ttttatctat taactcttga gctttatcta aaacttttga
     5401 tatatctttt tgagcttcca tttcaggtaa atctactttt aaatttttta aatcattaac
     5461 tgaaaaactt gcttgattaa ctgatttttt tattatcctt ggtaactgtc ttctaaattc
     5521 attactatta aaaaaataat ttaaatactt tggataacaa acttctttat ctggtcgtaa
     5581 acataaaaga ttcattccat gtataatttt tttatttata tcttcatata ttgcactttt
     5641 ccctaaatgt ttttcactat taatatgtga cattagtata tctccatttt tcaataaata
     5701 gtcttctaat tttcctaact cttttatgtc agcatatccc attttattag tatcaatata
     5761 agagtttgct atagtttcta ttcttgtaat tggatatcca aattttgtat caaattgttt
     5821 aatactagcc ccatttctaa taaattcaaa aacttcattc aatctatatt ccatactcta
     5881 cacctcaagc attctttcta attcctctaa tccatctacg atttccttct ctaactccct
     5941 aactctacct ataataacac taggagcatc ataaactact tcctcataca ctacctcttt
     6001 atacttatta atagataaat catacccatt ctctcttatc tcttccacat caacaaaaaa
     6061 gctttgctct gttctttttc tatctacttc accatcaaga ttatcaaatc tcttaataat
     6121 atcctctata tcactatcct ttatctcatt tctcttatca tccaaagaat aaccatcagc
     6181 tttcatatca tagaaccata ccttatcagt tccacctgtt cctgttttag taaatatcat
     6241 tatagctgta gatactcctg cataaggctt aaacactcca cttggcattg atattatagc
     6301 ttccaattta tgattatcaa ctatttcttt tcttatcgat ttatgtccac ctgttgaacc
     6361 aaacaatact ccatctggca ctatacatgc gcatcttcca ccaattttta aaactcttaa
     6421 aaaaagtgcc aagaataata actctgtttt ctttgtctta gtcactttta ataaatctgc
     6481 tgacactgct tcataatcta aacttccctt aaaaggagga tttgctaaca ccaatgtaaa
     6541 cttatcttta tctgtattaa cctccgataa agaatcctta tactctatat taggattgtc
     6601 aacgccatgc aacatcatat tcatagcacc tattctaagc atagttctat ccatatcaaa
     6661 tccatagaac ataccattat taaaatgctc cttcagccct tgcactaaga aaagacttga
     6721 atgattatcc cttaagtatt gttgtgatga tactaaaaaa cctgccgaac ctgctgccgg
     6781 gtctactata atatcttctg gtgttggttt aataagtctt actatcatat ctattatgtg
     6841 tcttggagtc ctaaattgac cattagtacc tgctgtagcc actttggata ataaatactc
     6901 atacaaatct ccctttgtat ctctatcctt acttaattct aaattactaa taccatcaac
     6961 tattttagat aacataagag gcgtaggaat tttaaatatt gcatctccca tgtactttgc
     7021 ataagctgat tctttatttc catgtaattt ttttatgaaa gggaacactt cattttgtac
     7081 tatttcatac attttttctg aatttccttc attagaaaac ttactccacc ttaaatgttg
     7141 atgttcatca tcaaatatct tttcaaaaga aacacccaaa agagttgctt ctgcttcatt
     7201 ttttgtttct acttcatcca atcctttaat aaataaaaga tatgtaaatt gttctataac
     7261 ttctaatgga ttagttaatc caccagtcca aaaagtctcc caaatcttgt ccactttact
     7321 ccttaattct cctgtaatca tatatgactt tccctccagt gttcataaaa taatttaaat
     7381 atatatttta gtcgtacgcc ttaatagtta gtgtcttatg ttaaatccaa gtttttacta
     7441 cacaattatt aaattatttt tcctatttac atattttagc taaaaaacta atgcaatact
     7501 cttattttat gattattata tataccatac aaaattattt tatcatatat ccatcataga
     7561 agtcatctac ctggataact cttaccatat tataactttt taatattaat ttaaacttag
     7621 aaagaaattt tttatttaat tatatcatct tccttatttc aaatttctca attacaatct
     7681 taatcaaaaa ataaaataag atgctgtctc aaaatagata taatcttctt ttttattgtc
     7741 ggaatgatga aaatatattt aatttcatac aaaaaagagt gcctcatgaa ctaaaagttc
     7801 attttgagac accccttaat ttaatcttac ttattctttc cctttattat taacacttca
     7861 ctatctctat tgcttttcct acacatccac aaacttccat cctactctta acaaaatctt
     7921 taactgactc taaacctttt ttaccatatt ttttagggtc aattacctct gggtcagaat
     7981 taagcacatc cttaacccca ttgctataag caatccttaa ttcagttgca taattaacct
     8041 tgcaaatccc tctttttata gactccttaa taacctcatc tggtattcca gacccaccat
     8101 gtaatactaa tggaactgat acaacctctc taatttcaga taatctatct aaatcaagtt
     8161 ttggctctcc cttatatagc ccatgtgccg tacctattgc tactgctaaa gatgataccc
     8221 ctgttctctc tacaaactct tttgcttcta atgggtctgt atatgctcca ccatcaccac
     8281 catctaaatc gtcttcctta cctccaactt tacctaattc agcttcaaca ggtatatctg
     8341 aaggcataca tgcatctaca acagatttag atattgaaat attatcttca taactttcat
     8401 gagaaccatc aatcataata gaagtatatc caactctaag tgcctgcata gctagtccaa
     8461 agcttgaacc atggtctaag tgcattgcta ctggaacact tgccttctct gatgctacct
     8521 ttacattcgc taaataataa tctagtcctg catactttat tgttgaaggt gttgtttgca
     8581 atattactgg tgatttaagt tcttcagcag cctctattac tgccataacc atctccatat
     8641 tttcaacatt aaatgcacct actgcataat gcccttcttg tgcatctaat aatatttgtt
     8701 tagttgtcac taatgccata atttctcctc ctatatgttt atactgattt aatttcttcg
     8761 ttatttaatt ttgatactat ttctctactt gttttgctat ttaataactc atttaaaaat
     8821 tcttcattca ttaattttga agatagttga gataatgttt ttaagtgtaa attatcacca
     8881 ttatctgtag ctgctatcat aaatataata tgtgccaagc ttccatcttc tgattcaaag
     8941 tcaaaccctt cttttgatag acctactgct acacgtggta cctttactgc atctgttttt
     9001 gcatgtggta tcgctatccc cataccaaaa cctgtagatg atatttcttc tcttttaagg
     9061 atttcttctt taaacttctt tttatctaaa agaacactag atttatcaag tattccaatc
     9121 atttcatcaa ttacatctgt tttatttttt cccttaacat ctaaaattac tgtttcttcg
     9181 ctatataaag agatattatt agatacgttt tccgattctg cactttcatt tttatgttca
     9241 tcttttgtta catttgtatt ttctatagta tttttctcat ttactacttt aggtgtaacc
     9301 atcatataaa gtacagcacc tactaatgaa cctacaaata tacaccctac ccataaaagt
     9361 ggcttattga ctaatcctag aaccaaaaac cctccatgtg gagctggtac ttctattttc
     9421 attatataag ttaaaattga tgatattgat gatgcaatca taagtactgg caacactttt
     9481 aatggatttt ttgctgcaaa aggtattgcc ccctctgtta tatgagtact acctagtata
     9541 taattgatta atcctgctgc tctatcttct tctgtaaatc tatttttaaa taaagtagat
     9601 gctattgcta ttaccaatgg tggtgttata caagctgcag atacacctgc cataaataaa
     9661 taatttccct gtcctagaag catagttcca gttacatatg ctgctttatt tactggacct
     9721 cccatatcaa atgctgacat acatcctatt actattccca aaattatagg gcttgaattt
     9781 tgtaaccccg atagaaagtt catcatagca ttgttaagtg ctgaacatgg cccaccaagt
     9841 aacaacattg ctacacccac aagacctacg ctgacaatag gaattataaa aatcgattta
     9901 agaccttcta attgtcgtgg taattttctt aacaaatgta caaatagttt aactaagtaa
     9961 cctgctaaaa atcctgatat gattccacct aaaaaccccg aacctgtatc aaatgatatc
    10021 attcctccaa caagtcctgc caacatacca gctcttcccg atatagattg agctataaat
    10081 gctgccaata caggaaccat caagttcata gaatatccac caacagattt taatgtagct
    10141 gctatttgat tatattgttc tgaatttggg tcaaatgaat aaatccccca taaaaatgat
    10201 atagcagtaa gtactccacc tgctactact aaaggtaaca tgtgagatac accattcatt
    10261 aagtctttat acaacttttg accaaacgat aaatttcctt cttctgcaat tttactgctt
    10321 gataagtttc cttttctaat agctactttt ccatcaatac atctttgtat aagttcatta
    10381 gctttatgta taccttcttt gacaggtact tctattactg gctttccatt gaatctgtcc
    10441 atatcaacat ttttatcaca agcaactatt attgcatctg ccatttcgat atcttttgct
    10501 gttaacttat tttctacacc tatagctcca ttcgtttcaa cttttatgtc acaccctaat
    10561 tccctggctg aattttttaa tgcttcttct gccataaaag tatgagctat tccagttgga
    10621 caacctgtta cccctagaat cattttcttt cccattttta taccaccttt ctgactctta
    10681 tattttttat ataatcttcc acattctcca aattacctat accattactt tcagccacac
    10741 ttgctcctgt tgctgctgac ctttttaatg cttcttcaat atgttctgga ttttccaacc
    10801 atatacttaa aaaagcagct aatgcagaat caccagcaca tgcagagctg actactgtaa
    10861 ctggttgagc actagcataa taaattgatt ttccattata aaaataagaa cctttttctc
    10921 ctaaagttag caatatattt tgagcacctt ttaaatgtag taatttaagt acatccttaa
    10981 tatcttcttc atctcttaca ataattccaa atatatcttt gatttcttca tcatttggtt
    11041 ttatcaaata tggattatac tgtagtaact cctttaattt tggtgaactt atatcgagta
    11101 ttgtctttat acttttattt ttacaaattt taaatatatc ctcatagtaa ttatcatcaa
    11161 taccaggagg tagacttcca cttattgata agtaacttat atcttctaat gactgtatct
    11221 tatctagcat atctagtttt tgtttttcgt ttacatatga cccagagttt acaaatttaa
    11281 attcatcact tccatcattt aaaaatatat ttatacgagt tgtatcttct acccaagtag
    11341 gcaatacgtc aaatcctctc ttttcgcttt cttcaactat atattttcct gaaaatcctc
    11401 caaaaaatcc taatatctta gattctatac cataatgacc tagtacaaaa gttacattta
    11461 atccctttcc attaggagaa tatacagcat ttgatgtacg atttactaat tttctcttta
    11521 tcccattagt agatatattc atatctattg caggattcgt agttaatgta tatatcattg
    11581 tcttccccct tgcttgtgct gtgtatgttt tcattataat aaaaaagtct atgaaaatct
    11641 tttcacaaat agaaaatcga ttttcataga cttttcaatt taattttcag aattattata
    11701 tctgaccata taatctataa ttatcctaga tataatatac aagggcaatc tggaagatac
    11761 ttcatattca gaaattgata tatgttcatg tttgtatcct acaatatata aatccgaata
    11821 ctttataagt tttgcacttt cactacaaca gcaagttata accttagctc caactaaatt
    11881 ggcattctgt gctgattcta caagctctat ggtatttcca ttcaatgaaa atataattat
    11941 aagttcgtct ttttttattg atttagattt tattttcatt atgttggggt catttataaa
    12001 aacaacatta aaatcaagta attgcaattt aagactaaac tcttttgcta catattcagt
    12061 aagccctctt gctaaaatat atatcttact agatttattt atagttttta ttatattgat
    12121 tagtgtagtt aaagatattt gttctataac tctctgtgct tctataaagg atttatttat
    12181 tatatccccc atattttgta tatcatcatt ttcatctttt actgttaatc tatacctaag
    12241 ttcattgaat ccatttaaac cacattttct aattgctctt gaaacggttg ctggagatga
    12301 atatgtttca tgagcaatct ctactataga taattctggt aatattgatt cattttcatt
    12361 tataaattta attatttcaa gttccgtctt cgttagattt tcagaattac ttttgtttag
    12421 ctcaattatc atatgttatc tccccttata taagttttat ttgtctaaat ttatcgagta
    12481 atataatata ttacctctta actacttatt tttatataca attaagattt agtcaatgtc
    12541 tatatatgta aatttataat ataataattt tttgcttaaa gtttattatg ttatcttata
    12601 gcattttaaa ataaaaaaga attgtacctc taccaaaatt tggatgaagt acaatagata
    12661 ttcctcatac tgatatgttt atgattaaaa gtataaatat aaatttcgct atgaatcata
    12721 taattaataa atcattttat ttacaattat aacccccctt ataaaactat attaataata
    12781 tatacagtat ttataagggg ggttgtttat atttaaaaac tatttacttt tctcttaatt
    12841 cttctccatt agttcttatt acattttgat accaataaaa actatctttt ttataacgtt
    12901 ctttacttcc atttccattg ttatctatat ccacatatat aaatccatat cttttttcca
    12961 tttcttgaga tgaacaactt actatatcta ttggtgacca catacaataa gctaatattt
    13021 cggttccatc agctatagct tcacctattt gttctatatg ttttcctaaa taatctattc
    13081 tgtaactatc atgaactttt ccatcttcaa atttatcatg cattccaaat ccattttcag
    13141 ctatcattat tggcttttgg tatctatcat aatattgaga taaagtattg tataatcctt
    13201 ttgggtcaat tgcccacccc caaggatttg cttttaaata tggattcttt atattgtcag
    13261 ttggattcat ggtgttttta cttgctgata ctgttgtaga ataatagtat gataaagcta
    13321 gaaaatccat agtattttct tttaagattt tatcatcacc ttcctccata actatattta
    13381 tgttattttc tttgaaaaat ctcttaatat agactggata ctctccccta aaacttacat
    13441 cagtgaaaaa atattgtagc ctatttcttt ttaaagcaaa tactacattt tcagggtcac
    13501 aatcttctgg ataagatgta caatctgcca tcatcatacc tatcatcata tctgaattta
    13561 tttcttttcc aagcttctta gtcaaagctg tagctatgaa ttgatgatga atagcttgat
    13621 atctagtctc ttctacatta tctacttggt ctacacaaaa tgcaactgaa ttaaatgatt
    13681 cataatgtat taaatttatt tgatttataa ctatccaata tttaacttta tttttatatc
    13741 tatttagtat taattctgca tatctaacaa agaaatctac cacttttcta ttagaccatc
    13801 ctttatattt taatactaaa ttcaaaggag tctcatagtg aagtagtgtt acaataggtt
    13861 ccataccatt atctattact tcatttataa gattatcata aaactttaat ccctcttcat
    13921 taggctctaa atcatctcca tttggaaata ttctagccca atttattgaa aatctaaaac
    13981 actttaaccc tagctctttc aaaagcttta aatcttcttt gtatgtatga taaaagtcta
    14041 ttccactacg cttaggataa tatccattat tatctttcat agcaaacttt attctttcta
    14101 tagtcatatc agactcataa ggtttagata tatctctatc tttatcatac atatgtacat
    14161 cagctaaaca ccaacctttg ccaccttcat tccatgcacc ttctaattga ttagctgcta
    14221 cagctcctcc ccataaaaaa cctttaggaa acttacccaa tttaatcact ccttatttta
    14281 tttatttaaa tcatttacta ttatttacct tatattctat agactttact attcctacta
    14341 aagacatagt tatatttaac tcgctgaata tggtcataag agtatcttga gcatgtgcaa
    14401 ataacagcga atgtgctctt ttctctccac cagcttcccc ttgtataatc ttggtttgag
    14461 ctctatgtgc ttctgtcata aacccttttg ctttagctag ttttaaatca gcatttctat
    14521 aatcaaactt ttccaaatca ttaagagcat gtttaatttc tgttctagcc tcacctgcac
    14581 gaagtattat ttgcattgcc acctctacta attcttcatc tattatatgt tcttcaattg
    14641 tcatatttat aatttccttt caatttctaa atttaaacct cttggctctc taattttatc
    14701 tcttctttct cataagcttt aaaaaatgga taccatatca taaatagcaa tactatattt
    14761 attgctaaaa caataagacc tctaacatct tgatttaccg taaaagtaga tataccaaaa
    14821 ggaacatacc ataaattaaa caccttactt ggaattgtca caaatcccat actcattgat
    14881 aagtaagtag ttattgatat tactactgtt cctatccaca taggtaacat taaaaatggg
    14941 ttccatgcca ctggagctcc aaatattact ggttcattta tattcattat agaagggaca
    15001 atacatgctc ttcctattgc ttttattctt ccaactctgg ataaacacat cattataact
    15061 aatggtaatg tagcacccaa ccctcctaaa gttatccaac ctgtttgaat aacttcgtaa
    15121 acatatatat tagttgctac tcctcctgat gctactgctg ctgcattagc tgcaattgca
    15181 ccaagttgaa ttgaataagt tataggagat agtacccacc cacttattcc taaagaatat
    15241 ataaatacta ctaaaaagtt taagagtaca aatccaggta atgtctgtgc tatatctgat
    15301 actggtttaa atactctaac tattatttca aacatgtcaa agttgagtcc ataaattaat
    15361 ccccagccta caaacattat cactgttata ggtaacatat tatcaaacca cttgcttaca
    15421 aaatccggaa gtgctgaatc ttctccgaaa aatgaaaaag aaccaaataa ggacattatt
    15481 aatccagaga ataatcctgc aactagcgat acaagcattc cttctcctcc aaacctgttg
    15541 aagttaaata tagctccttc attcgtaatc tctggacgaa tcatcattaa cattaatcca
    15601 gcacctgcaa gaccagcgac tacactcata ctttcaaatt tcttattctt cattatttcc
    15661 ataggcaata aaaatgctat aaataatcct aaaagtccaa atgtatattg atttattgga
    15721 cttaaatcag gtaaaaagct tatgtaattc ttcaagacag aaactatagt tactaatgag
    15781 cctacaaata ccattggcaa aactttcatt atagttgatt gaactgattg tacccatata
    15841 ttcttagtca tattatttac aacaggagta aatttttcac ttaaaaaatc tataaattta
    15901 ttcataaaca ctctccccat aaatatttta tttgtttgat attagttcta gtgcttcttc
    15961 caaagccttt gccccatcta gtcttccata tgcagtttta gatatacatg ctactggaac
    16021 gttatattca ttagcctttt gttgaatttc ttccttcata taatctaaat gtggtcctag
    16081 taataaacaa tctatttctg ataaatactt atctatctct gactcacttc tcgcttttat
    16141 atctgcttct ataccttttt tctttgcact ctttctcata ctagttgcca taaaacctgt
    16201 acttgctcct gaaccacacg ccaaaagcac tcttatattt ttcataatga tacctccaat
    16261 atttttatcc atcaatttat ttcttaatga actttttatt ttattatgtt tatattgtac
    16321 ttaccaaata agttcatttc acataaatat atttcctaat aataggaaat cagttactct
    16381 taattaaaca tattaatata gtataatgta tcttataata cgtacattca attatataat
    16441 tatcatatca tccagatttt attttaaaac tatgcgaggg aatctaatga aaacatctaa
    16501 aaaagaacta tttttaaatt tcataaaaac acaacatgat tggatagatt catctacatt
    16561 ggctaattat cttaatgtct caacgcgttc aattagaaaa tatgtaaacg aaattaattc
    16621 caatggtgaa tttatattat cctctaaaaa aggatataaa gtaaatctca ataataattg
    16681 tcaaaccaag gttgatagtt cagaaaatat atcacctgat aacaggttaa atttaatttt
    16741 aaaagaactt atagttaata gtaatggaat taatatattt gatttaagtg aagacttgtt
    16801 tgttagtcca gctactattg aaggagatat agtcaaagct aataaattta taggaagtta
    16861 taatttaaag ataaagcaat cgaaattttt attgaaacta attggaaatg agtctgcaaa
    16921 gcgcaaatta atgagctcta taatatttaa agaaacggga agtgattttt taagcttatt
    16981 tgatgtacaa aaaatatatc aagagtataa tttgaccaaa ctgaaagaaa atataatata
    17041 tatattaaaa aaatacaatt tgtttataaa tgagtatgct ataaataaca tacttcttca
    17101 tttaatgata actatagaca gaataaaaaa gaataactac atagaatctg tcgaagtagt
    17161 aaactacata gataataata atgatataag catttcaaga gatatatcaa attttttaga
    17221 gtctgaatac aatataactt taactagtgc agaattatac tacttagtat ttcagttaac
    17281 aaacaaaacc acagtcttaa attatcatga aatggatact aagtcattgc caaattatat
    17341 agatgaacat tttgttaaat taactaaaaa aataattaaa aatgtatatg acttatactt
    17401 tatagattta tctgatgatg aatttgttgt aaaatttact cttcatgtaa aaaatttaat
    17461 cagtagggct aaaaataatc aagttttgag aaatcaaata cctcagaagt taaaagattc
    17521 ctaccctctg atatatgata tatctgttta tatttgtaat cagattcaaa ttttagaaaa
    17581 catagaaata gatgaagatg aaatatcgta tatatctctt catgtaggtt cattttttga
    17641 tagacaaaaa ttactagaag ataaaattct ctgtgcatta attactccaa attactatga
    17701 tttacaattc aaaatagtaa gagaccttga gaagagattt aatgaatcca tagaaattat
    17761 tcagattttt tcagatactc ataatcttga ttttgacaat aaggtagaca tggttattac
    17821 tactttacca attagcgata gatgccctat accttttgtt tatgtaaatc cttacctaaa
    17881 tagaaaggat tatgataata ttcaaagtaa atttattcag attaaagata gaaaaaatat
    17941 acttactgta caaaatcatt tagaaatgta ctttagtgaa tctttgttta tgaaaaatat
    18001 atatttagat agtgctaaag attatataag atttatggga aatattctgt acgaaaataa
    18061 atatgtcaaa cccaattata tagatgatgt tcttataaga gaaaaaatgt cttcaactgc
    18121 atttaataac aatgtagcaa ttcctcatag catgaaaatg gatgctttaa aaaccggagt
    18181 atgtttaatt gttaacgata aaccagttaa atggggtgag gaaaaagttc aaattatagc
    18241 tatgatacct ataaatgaaa aagaaaaaga aaagttcaat tacatatttg aaagctttat
    18301 tgaaatatta tctgagtgga ataatgttaa agaacttact aaggcagata actatagtag
    18361 ttttatgaat agaatagctt atcttattca aaatatctaa atcaattaat ttatttccaa
    18421 tatatgtttc ttaagttatt aacataaaat agataagctg tcacactaaa aagtctaaat
    18481 taatatgtga cagcttttta cctaatatac cttgttttaa tactacatat tttttgactg
    18541 ttgtatacta gttataagat tgaatatttg tgttgttgta gctgtcatat tctcactagc
    18601 agttgcttta tccattgctt cttctattgt catagctcta tttactatag aaaaataagc
    18661 atctataccc tcttcattac atttaactgc atcttctgtt gtggctccag ccaaacctat
    18721 tacttttacc ccatattttt ttgccagttt tgcaactcct attggagcct tgcccatagc
    18781 agtttgattg tccaatctac cttcaccagt tatgacaaaa tctgcatctt ttaactcttc
    18841 ctctaattta atttcttcta atattatttt tattccagat tctaattttg aatttaaaaa
    18901 tgctaaaaaa gcaaaaccta atcctcctgc tgctcctgct ccttctacat tagctacatc
    18961 tttacttaag taatttttaa ctactttgga aaaatttcta agcccattat ctaactcctc
    19021 aattatttct gatgttgcac ctttttgaga cccgtatatg tatgcagctc catttttccc
    19081 aaacagcgga ttatttacat cacaggctat tttaaactta cattcatcta attcctttaa
    19141 tctattttct atttttatat gtcttatttc atttaaaact tttccaccta atcctactaa
    19201 cttgttattt tcatcataaa actcaaaccc taaggcttga agcataccta tcccagcatc
    19261 attagtagca cttccaccta tcccaactat aaaatttcta tagcctttat ttattgcttc
    19321 ttttattatt tccccaacac cataagttgt agtgtataaa ggatttctaa gttcagtcgg
    19381 aaccaatgtt agtcctgatg cttgagctat ttctattatt gctgtacttg tattttttaa
    19441 taatccatat gttgcatcca cttttttagc tattggtcca gtaacttcta tagtatcttt
    19501 tttaccattc atcccttgaa ctaaggcatc tactgtccct tcaccaccat cagcaagtgg
    19561 caatatcttt acttgcgcat cttctttaac cttcaatatc ccctttttta ttgcatttcc
    19621 tgcctctatt gaagataagc ttcctttcaa agaatctata gaaattaata ttttcatatt
    19681 tatctactcc tttttattac tccttatttt aaacatataa ttttataagc ttaatttatc
    19741 ttttatttta tactctacat ctctctttgt atacaactta taccattaat attcttttat
    19801 cttataaaaa gggagtgtct caaaataaat ttcttagttc atgagacact ctttttgtat
    19861 aaaatcaaat atatgtttat tatttcaaca atgataagga ggattatctc tattttaaga
    19921 taattccttt ttagcactat ttaaccagta tagttttaac ttggttcatt aagcactcta
    19981 catttacaga gttattatct attgatatag attctaattc tttttcattt aggttaatcc
    20041 atttagctgt tttaatattt ttgttaaata ctatgttagc tgattttgtt tcttctttag
    20101 aattgtaaaa tctaactact aatgcctctt gtttttcagc cttttttaca acacttaaca
    20161 caaggtcctt attagtttcc ttaagtaaac tatatgaata atctacttct atttctgatt
    20221 gattcaattt catagcatta tatggcattt tattataagt ttgtattttt gttaaatact
    20281 ctttagccat atttgcaaca tttgcttcta gtgtagattt ttcatgagta actattgcta
    20341 agtctatttt tatatctcct atcatttgag aatctggagt aggcaacttt attcctgatg
    20401 gacgacctgg gcgtcttgcc atttcttctt tacctaggaa tcctacactt ctaaataatg
    20461 ttatagctat tgtgtcaaat ttttccccta ctatttcaaa ctctcttgta ctgtttgtaa
    20521 gaacagctac tccatgctct ttgtcagaca atccaacgaa tgttagcatt ggatatatag
    20581 aatctggtct ttcactccat tcttcctgtt gccaaacttc catagcatca tcatacacac
    20641 ttctctttat atgtccaaac tgattatcag acacagaaaa ctttgaagct atatttgttg
    20701 gtatataagt tctaacccta caatctcttg cttggttatt tatgttgaat actacatcta
    20761 ttatagcttt atcatttgga acatttataa ctatatcaaa gtcaataaat gaatctgctc
    20821 tattattttt tctagcttgt atatcttttg gtacttttaa tctataattt atctttatct
    20881 cattattaaa tctatttttc tttatagtgg aatttgctac tatattatcg ttaaatatta
    20941 atttctcatt tggaagtggt gaaaaatcat actcatctcc ttcatctcct ccattttcca
    21001 tcaataatac attttcaaaa gttttattta attttttatc aaatatcttt aatgtcccgt
    21061 tttcattaac ttgaatagta taaaagtcat tatctaccat gtcacatact tcataagctt
    21121 tttctaacat atgttcaact tcttttatca tatacgcctt ataccccatt gcaggtattt
    21181 tatctttaaa gtttattgta tataccataa atgaatcata gtttccataa tgaacaattt
    21241 gacggtctat taatcctgca tctattactt ctttatgtac cacttcaaaa tccaacttat
    21301 tattatcttt atcaaatatt tcaaatgctt tcattttagt tataacttga gcgcttacta
    21361 tttcattcct atcgtaaggc attaagttaa atgctactaa cttatctaaa gccatttcac
    21421 aactcattgc atcaactatt cttcttttgt aaaatgttat taattggtct actttttcct
    21481 cagctaagaa aaatctattc attatttcat ggtgaacttt atctgaacaa caacatccaa
    21541 tgctgtcatg agcatgattt ttcattattt ctttccaaat taactctatt aatccatgat
    21601 gatattcaaa tcctagagaa tatgcaatag aagctagtgg ctccaatatg ttggttatct
    21661 ttgattctat tctagcattt gctgatttta tatccattct tgttgagaat atacttctat
    21721 gaaccctcat atatttccca tctaaaaatt ccccttttat tgtatctata tcctcatttt
    21781 tttctagctc tttaaatatg ttttcatatc tacttaagaa aaattttctc tctggataac
    21841 attcttttaa tttatatata acttcaaata tattcttttg aattggcatt tggtcatgtc
    21901 catttggaag tattatatgg tcagtagttg cacccttatc aagaaccggt aaatatttat
    21961 ccattcttgt tcttagctca tcttcattca ttggtagata ttttcctatt gcataaccta
    22021 atggaagtat ttgaaccaaa acagaagacc catcatcact cttccaatta aactctgttt
    22081 tattagttcc ttttctttca gaacatcctc tccaaaacat tgagtatttt atatcaaacc
    22141 catttagtat ttgtggcatt tgagcacttt gaccaaatga atctggtaaa tatcctattt
    22201 tcatatattc tccaaattca tcacaatctt ttattccata tagtaaattt ctaagtatgg
    22261 attctccacc aactaccatt tcatcagttt gagtatacca aggacctatt attagccttc
    22321 cttcttgaac taattttttt attctctcct tacattcagg cttaactgct aaataatctt
    22381 ctaatatagc agtttgacca tccatcacat aatatggata gttatgatta ttttctaaca
    22441 tatccattat ttcttccata ttatttacaa gtaatattct agattcctca gttgagaagt
    22501 accattctct atcccaatgc atatgaggaa ctatgtgtac attttttttc attgcttatc
    22561 ctccaaaact tatctgataa tattttctgt ctaattaaaa atccaataaa aatactactg
    22621 agtttacaaa attttttaat atttatttta tcactataat taaacctttg atttaaatat
    22681 ctaataacgt atcttttaat gtatctttct ttgatttttt tgtttcctag ttattattaa
    22741 taatactgct gatattatag ctccaagtac tgccgcaaag aaccatattg atgctataat
    22801 aaatcctgac atacttccct ctaaaagcgc caatgagaat atccccgctc caggcacttg
    22861 aagacctata ttgaagaatg ctactataga cccagttatg gctgaaccag ctaccaatga
    22921 tggtataaca cggattgggt cctctatcat aaatggtatt gcaccttcag ttattccagc
    22981 caatcctaat atccaagttt gctttcctat ttccttttct gattctgaaa ataaatctct
    23041 gcctaaaaca gtagctattg taactccaaa agctgaaacc atcttaactg atgcaaatat
    23101 tgcataaggt ataaaatttc cacttgctat tgctcctatt gcaaatgtat aggctgcttt
    23161 atttattggt ccacctaagt caaatgaaac catagctcct attattgcac ctagtagtat
    23221 tgcatttact ccagtcatcc catttaacca atttataaga ccattgttaa gtgctgctac
    23281 aggctttcct actacaaata acatcaataa acctactacc aaagaaccaa ctactggata
    23341 tacccaaaaa cttacgaatc cagctaatgt gcctttggct ggtatattct tctttaaaaa
    23401 ttttactata taaccagcaa gtaaacctcc taacatacct ccaagaaatc cactacctat
    23461 taaatcagct gctagacctg ctgcaaatcc tggtccaaga cctggttttt cagctagtga
    23521 gtaagacata tatgctgata atactggaac catcaatgta cctaacatac ctccagaaag
    23581 ctttcttaat tgccataacc aagaaccttc ttgattgtat agttcttgta gaccaaatgc
    23641 ttgagatgct aatactgcaa atgcaagtat catacctcct gccacaataa gtggtattat
    23701 atgagatact cctgtaagta ttgctgattt tacatctttt ttccatgatg atttttcaca
    23761 atatgatata tcagatgatt tttctatata atcaccttta ccttgttttt gtgctacttc
    23821 taaagcttcg tatattattt cttttgctct ttttagtgga gctgctactg acgtttttat
    23881 gtttggcaaa tgttcaaatc tttctgtatt tttaggtgct acatctgttg catatattat
    23941 tgcatctgcc ctttttatta tatctggtgt atgagggtcc accataccat tagctccttg
    24001 cttttctaca tacacatcca caccgatttc tttaccagct ttaattaatg cttctgctga
    24061 catatatgta tgtgctatac ctgcagggca tgctgttatt gctaatataa ttttttttga
    24121 ttcattttga atcctaatct cttcttttat ttgttctttc ttttctttta tatctaagtt
    24181 attaaataac tctttcttag tttttgaatt taacaacata ttcatataat cttcatttga
    24241 cagtctagta actaactcag aaagtatttg tatgtgtgta tttccacctt cattttctgg
    24301 tactgctatt aaaaatatta tctctacttt attatttggg tctacgcttt cccaattttc
    24361 tattggtgtc tctaaggtca tagctgcaaa tgcagcttct ttaactgact tagattttcc
    24421 atgaggaatt gcaagccctc tttcaaaacc tgttggagat aaagtttctc tgtacattac
    24481 agcttctaaa tattcctcat aagatgttaa cttgccttca tcatatagct ttcttgctaa
    24541 gaactttatt gcgtctttct ttgtttttat tttttgtttt aaaaaaacaa ggttttcatt
    24601 tgtgattttc ttaaattcca aaattacgcc ctccttaaat catttaatgc tatgaatata
    24661 gcataaaata aattatgaaa atgttttttt gaaattatac acacaatttc ttcttaaaaa
    24721 ataaagattt gtgtatttat acacaaatct ctatcttaac cagtattgct ctattattgc
    24781 tctcataatc atcataattg gaaccttatc tgatatattg tacccattta cttgtttaat
    24841 tgttgaatag caatataaac ttatatctgc aatttgttgc aattgatttt cacatatatg
    24901 tgttagagct actaccttac aacccttatt aactgctgtc tttgctactt ctataacttc
    24961 tttcgtttct ccacttaaac taagtgcaaa aaacatatct tgttcaccta attttcctat
    25021 atcatgaagt acttcatgtc tttgactata gtaagttata ttatcatgcc cacctctaat
    25081 ttcttttact aatatctcac ataaaggaat gttttgacct attccataaa ctataatact
    25141 cctagatttt tgaattatat ctgctacttt atttattttt tcataatcta ttagctctaa
    25201 tgtctttttt atatctgatt caaaaccatt ttttggctct ttatcctcat tttttaactc
    25261 ttcttttaaa ttatttttta gttgagagaa accatcatac tccatttttt tacatagcct
    25321 aactatggta tttgggacag taaaaaattt atctgctaaa gattgtattg ataattttac
    25381 tatttcttct ttgttttttc tcatatattc aattatcatg tcttctgtat ctgtaaattt
    25441 atactcatat ttttttgctc tttcataaaa ttccatagtt tctcctccat ctattttatt
    25501 attcataagt aaaattcaaa aatattagct aaaattttcg ttaatataat tataatttaa
    25561 tatttttaat ttattaatgc atactactct ttttattata cataaaaaat gtgtacttcc
    25621 tatattgata gagatgattc aagtttttat tttgatacat ctctatcaat atttttttag
    25681 aaaatacaca tttttttcaa atacttattt ttaattaaaa ctttaggtaa tctattcttt
    25741 atatataatt attagacttg aaaatatctt taaaatcaac caattcaata ttatttcttt
    25801 ttaaccacat tttaacttcc ttatcacata gcattgcatg ttcatatact ctctcttgaa
    25861 taagagaaga tgattttata agactataat ctataaatcc tggatgagta accatcaatg
    25921 ttatatcttt atctaaattt atatattttt cttcaaataa tttaacaaaa gatttattta
    25981 acttgtaaaa atcattctga ggatatttac ttggcatgct aatacatgaa tccaaataat
    26041 agggacaact tggtataccg tattcttttg cagcatcaca cattgctgat attgatacat
    26101 cttcttctat accatgtata tcaatatact caggcaattt actcatatac aacttgtaag
    26161 tttcaatttg agcacaaact tcttcatata cttcttcata attaaaaacc tctttacctg
    26221 tcagtagttg cgttcttctt attttgctag aaacaaattt tccaccttca tctaacaaat
    26281 tctttatctt aatatgttct ttgcaacaag gttctcctat tactaaatta acatgtaaac
    26341 caaaacatac atcatcattt tttaccattt ctatagcttg tcttgaaaaa ggcatattca
    26401 ccatgattcc agcacttgta acaataccgt tcttataaga agatattatc cctagtgtaa
    26461 caccttctga atatcctata tcatctgccc taactactag tttcttcatt ttatgcgtct
    26521 gcctgctttc tcaactcatc aagttgcatt tccatctcat cctttacttt aacaacatcc
    26581 gttcctatga tgatttgaac tgatgtatta ctcaatttaa ttaccccttt ggctcctgtt
    26641 tttttaatat tatcatcatt caaaatagta gcatccttca cttctaaacg taatctcgtc
    26701 atacaattat taagagtcac tatattttca taaccaccac aatttttaat tatctgttta
    26761 gccatatatg aaaaattacc atgagaaagt tttaaattct tttctttttc actaacttta
    26821 tcacctatct ctatctcaac ttcacgtcct ggtgtttgca cattatcttt tattattata
    26881 tatttaaatg ttacataata taatacaaag aatacaactc ctacaggtat aactaaccac
    26941 gctttatctc cataactaaa acttaagaca tagtcaatta tacttgctcc attaaatgaa
    27001 cccaagcgaa cacccagtaa atatagtaca actccaccta gaccagaata aaaagaatga
    27061 attatatata actttggaga tgcaaataaa aacgaaaatt ctattggttc agttactgtt
    27121 gccataaaag aagtcaatgc tccagcggtc attgcacctt ttacttcttg cttcttttta
    27181 tcttgtgctg ttttatatat agcataagaa atagcaggta ttgaaaacat cataattatg
    27241 taaaatcctg tccagaagaa tcctgcattt gggtctcctg ccataaatct aggaatttct
    27301 cctcttataa cttctcctgt tgatgttgta tattcaccca tttcaaataa tatgtaagca
    27361 tttaaaactt gatgtaaccc agttggtatt agtgcacggt tacagaataa aaacaagaat
    27421 attcctattg caccagctcc tcccaaccaa aaaccaaatt gattcattgc agcttgtgga
    27481 tatatccata ataaactaaa tatatatgat aaaattaaag caaccactgg tgatagtgta
    27541 attacacatt tttctcctgc aaagaagctt aaaaatgctg gaactttaac tttatatgtt
    27601 ttattgtaag tccatgctat acaagctcca acaattatcc caccaaatac acctgcattt
    27661 aatgtatcat tcaataactc taaatttgac ttaaatatca tatatgcaac tacggctgca
    27721 accattacac ttgtcttgtc tttagacttt gagtaggcac tagctgctcc taataaaaat
    27781 aagtagtcca catttgaaaa tactatatta gatatattag atatagctgg cacattgagc
    27841 atatttggac caaataataa tcctaatata cctgctgctg gcattacagc tattggcact
    27901 agcatagtct ttcctaaaag tgacagttgt tgcatcattt tgtttaagaa cctcttcata
    27961 tccaatcccc tttctatttc ggtttaattt tttgtgaaaa cgatttattc acattttata
    28021 ttttaaatat tatatttgtt taaacaaatt gtcaatattt tttattcatt ttatttttat
    28081 catttagttt aacatataaa tccttttaag tgtattgcta tatctagtct atttttttaa
    28141 ttcatttttt ttattttaaa aatatgttta accatgtatt tgaaacaacc cacaaaataa
    28201 ttctattttg tcctttttat ggttgatatg attctatttt cactatacat gtctaaacac
    28261 atttgatata atttgttcat atcagttcaa taatattact aaagaccttt aaggagggat
    28321 aggggtatat ggctaaaaat aaattttcag agatatatga agtattgaaa gaggaaatac
    28381 ttgatggtaa atatacaagt aatatgatgc tgcctactga acttcaactt attgaaaggt
    28441 tttcatgtag cagaaacact gtacgtagag caatttctca attaaacaca gaaggatatg
    28501 ttcaaagtat aaaaggcaag ggtgttgtag tgttagaaaa tagttgttct aatgattttt
    28561 ttctaaatat gcacaatttc aaaggtgtag aatcgattgt agaagataaa aaggttaata
    28621 ctgctactag tgttctccac ttcagcaaaa tcttaattga taataagtta tctaaaaaga
    28681 caggttttaa agttggtagt gaagtatatt accttcacag actaagatat attgataaca
    28741 tacctaaaat tctcgacatt aactattttc tatgtagtat agttaaagac cttgatgttt
    28801 ctatagcaca aggctctatt tataaatata tagaagagtg tctaggtaca aaaattgttt
    28861 catccagaaa gatatttaaa attgaaaaag ctacagagct tgagttaaaa acgctacctc
    28921 taaatgatta taattgtgtt ggtgttatta aaaatagtgt atatactgat gatggaaagc
    28981 tttttgaata tactgaatca aagcatacac ctgaaacatt tgtattcatg gatgttactc
    29041 aaagatatta aaaaaattat gtttaattaa acataatttt tttacgttta gacactttat
    29101 cccatactaa cctcactatt ctatatacaa ttatgtgttt atacaaataa ttatttattg
    29161 tatattttag ctttaatatt agtttaatgc ttgacaattt gtttaaacat attataatta
    29221 acttataaat tacaggaggt atcaattatg aaaaattggt ggaaaaaagc aactgtctat
    29281 caaatatatc caaaaagttt caaagatagc aacaatgatg gtataggtga tataaatggt
    29341 attatagaaa aattagatta tctgtattat ttaggtgttg atttattatg gttgactcct
    29401 atgtacgttt ctcctcaaag agataatgga tatgatatag aagattatta taatatagac
    29461 ccaaaatatg ggacaatgag tgactttgaa aaactcttaa aagaagctca taaaagagac
    29521 ataaaaataa tgatggatat ggtcctaaac catacatcta ctgaacataa atggtttaaa
    29581 gaatctaaaa aaagcaaaga caatccatat cgtgattatt atttctggaa agatgctaaa
    29641 cctgatggtt ctgttcctaa taactggata tccagattta gtggaactgc ttggaaatat
    29701 gatgaaacca ctaaccaata ttatttacat ttatttgaag aaactcaagc tgatttaaat
    29761 tgggaaaatg aaaaggttcg tgaagaatgt tacaaaatac tagaattttg ggctgataaa
    29821 ggtattgatg gttttcgttt agatgtagtc aacctactct ccaaaacacc tggattacct
    29881 gatgacccta taacaggacc aaaaggagat ggaagaactc actacgctga tggtcctcgt
    29941 atacacgaat atttacataa tatgaatcaa aaagttttca aacctaaaaa tatagttact
    30001 gtaggtgaaa tgtcatctac tacacctgaa gagtgtatca actatactag agaaaatcga
    30061 gaagaactat ctatggtttt tagcttccat catatgaaaa cagattataa aggaggcgca
    30121 aaatggacta atgaaatgtt ctctctggat aaattaaaaa aagcacaatc tgactttcaa
    30181 tataaaatgt atgaaggaaa aggatggaat gctctatttt actctaatca cgaccaacct
    30241 agagctttat ctagatttgg agatgataga ttttttcgtc aagaaagtgc taagatgtta
    30301 gccataacac tatttggtct acaaggtact acatacatat atcaaggtga agaaataggt
    30361 atgacaaatg cctactttac taaaatagat gaatatgatg atgctgaaag taagaatgca
    30421 tactatcata tgattaaatc tggtattgat gaaaaggagg cattgcttat acttcaacaa
    30481 aaatccagag ataatgcccg tactcctatg caatgggata atactattta taaggggttt
    30541 tctaatcata aaccgtggtt aaaagttaat aatgacaata ttagtgtaga aaatgagctt
    30601 aatgattctc gttcagtatt ttatacttat caaagattaa tcaaatatcg taaacaatat
    30661 gatattttta cagaaggtac ttatagattg ttagatgaaa atcataaaaa tctatttgtt
    30721 tatgaaagag agtataaaaa ccaaaatctt cttgttataa gtaatttcac tcatgataat
    30781 gtagtatata aattacctga aacttatttg aataaattaa actacacaat acttgaaacc
    30841 aattacacta gagatataat tgaaaataaa atggaaataa aaccttacga aagtataatg
    30901 atatattata aataaagtta tttatgtaaa aataggtata acaccctggt gttataccaa
    30961 ttttactaaa ttcaaagtaa tgtttataga atatctatag attacaataa ttatttgact
    31021 tctacatcat tatatttctt tgaatatact ttagtcatat agaaatatat aggaactaca
    31081 attatcatcc cacctaatcc ccaaactatc ttcataggtt catcaatacc tgcttttatc
    31141 agtaataata tacttataat aactgcaaat attggtataa ttgctcctcc aggtattcta
    31201 aaatttacat ctttaccagc atacttcttt ctcaatatta gtacagctaa acatgtagga
    31261 atatattgaa taaatctaga tacaacactt atttgagata atgttgtgaa tgaacccgac
    31321 catgctatta gtaaagttcc tatgacagat actataattg ctatataagg agctccattt
    31381 ttattagttt gtcttataga cttaggcatt aatccttgtt caaccaaagc agcagcagac
    31441 cttggtgtaa atatggatga tgcaatattt atacctccaa cagatactat agttcctatt
    31501 gtaattatat acccacctat aggacctaac atcttaatgg cagcatctgc aacaggagta
    31561 gctgaccctg ctaagccatt acccaacacc cctatactta ttcccagtat aagcatatat
    31621 attactgata ctacaaacat taccattacc aatgctttag gtacattttt cttagggttc
    31681 tccatatctt ttgctgcaac agctaaagat tcaaatcctg taaaagcata aaataaagtt
    31741 atagatactg ctacaaaagc tgatgacgca ctttgacctt tttgtattat tacaaatggg
    31801 gtaaaattag aaccctttat aaagaataat cctacagcta taaataaaac tattggtaat
    31861 aattttccta tagtcatgat attatttaag tgcttagatt gtttcattcc cataaaattt
    31921 ataatcccaa gccctacaac taatatacca attattatat ttttaataag tggttctgct
    31981 gcttgtggcc atacagatga tagtgttaca gcaaagaaat ttgccatagt agcccaagca
    32041 atcatactaa ctatccactt cataaatcca acttcaaatc caacaaaatc tccaaatgcc
    32101 tcttttgcat aaagataggg ccctccatca gtatcaaacc aactacctgc ttcagcaaaa
    32161 cataaagcaa tagaaacagc taaaagcata caaaaaacta atactaatat acttgctgga
    32221 cctatatctg tataagcatc accaggtaac ccgaatatac ctgaccctat tattgcattg
    32281 attcctaata gtacaatact ccaaaaacct aacttaatgc ctttttccat tgttattccc
    32341 cccaaagcca acttaatata tttgtatttt ttatttagat gcttctttaa ctattgccat
    32401 aaatattttt ttcatattat cataatctct agtcatcatt tctggatgcc attgtatccc
    32461 tattacaaac tcacttcctt ccttttctat agcttcaatt aaaccgtctt tagatgtagc
    32521 tgaaacaata tatcccggtg ctactttgtt gacaactaag tgatgaaaac tattaactaa
    32581 aacttctttt tctcctagta tttcatataa ttttgtcccc tcctttatct gtactgtatg
    32641 tgtaggtaca ttagatagat gttgctgatt gtgtttaata taagcacctt caattaaaga
    32701 taaatcttga tacaaactac ctccttcagt tacatttata atttgttctc ctctacatat
    32761 acctaaaact ggttttttca tatctaaagc atgttttaat aatttcatat caaaactatc
    32821 tctctttggt gatattgccc ccaacttatt atgaggttct tctttccata tgagtggatt
    32881 tacatcttgt cccccagata gtataagcgc atctatatta gatacctgct cctttataat
    32941 ctcatcatca tatacaatcg gtacaatata tggtatagct ttacacataa ctactgattg
    33001 tatataatca ttatttacat aagccctttc atagccagga aacattcctc cttcatcaat
    33061 tagtaaatta cctgatattc ctattattgg atatttcttt ttagccaatt tcttcacccc
    33121 ttaacttatt tgtgaacaat aaaaaatgct acttctctta aagagcaagt agcataccaa
    33181 gattttattt atttgaacat tataatttat tttagaaata ctttttttga atacgagtaa
    33241 tataaataac tttatttact tatattttat tttaatcctg tttagtttga cttttttaaa
    33301 ttgttttaag tacaactaat aggtaaaata ggcatttaga ttttcctttt tatcaaccta
    33361 ttaaatactg tttaatatat tttttcccct tctatttaat tcacttccct ttctaccaga
    33421 ttcacatatt atcaaatcca gctcttttaa catattaagt attcctctta cctcaccttc
    33481 tgttatattg tagcctaatt catttattga attaacaata ccatttctac ctatattttt
    33541 attaagatta ttggcatttt ttaaacactc cattactaac tttactttct ctatagatgt
    33601 tctatccttt aatatgctta tttctttact aaaatcttca gttatattta ataagttatg
    33661 aggtaaatca tttattaaaa taacatcctc agaactcata atgttaatat acatagcagt
    33721 attttttaat tctcttatat tacctggcca ttcatatgca ttaataatgt cttgaacatc
    33781 ttgactaatt gctctcttcc ttttcataaa atgctttaac ataacttcta tatcttcttt
    33841 tcgctctctt aaaggtggta tatttatggg caatacattt atcctatagt ataaatctgc
    33901 tctaaatcta tctcctttta tcatttctaa taaattttta tttgtagccg caattactct
    33961 aacatctata tctattacat tttctcctcc aaccctcatt acttgattct cttgtaatac
    34021 tctaagtaat ttgctttgta agtatatagg catgtctcct atttcatcca aaaatatagt
    34081 tccattgtta gcctgttcaa ataatccttt tttacctcct tttaaagctc ctgtaaaagc
    34141 accttcttca tatccaaaaa gttcactttc aagtaaattt tctggcatag ctgcacagtt
    34201 tattgctata aatggctgac tatgtcttgg tgatgcatta tgaatagact gagccatgag
    34261 ttcttttcct gttccacttt ctcccgttat aagaattgta taatcagatt cagctatttt
    34321 catacctaat ttcttagttc tttccatctt cgttgaaact gtttttatat catcaaaagt
    34381 atatctagca acttgtccct tttctctaag cttctttgat aggttttgct ccaattttct
    34441 tatgtaagtt atttcttgaa tacaaaaata atatccagta acccttccaa aactctctat
    34501 attatactta tttacgttta agtacttatt attaaatttt ataacttcat catgtgcctt
    34561 actcaaacta aaaatctttt taatattctc attctctaat tcatctatat ttttccctat
    34621 aatattactt tgaatatcta gtatatccct taaactatta ttacaaataa ttatgtttcc
    34681 cttatctgat accattgcca tacctaagtt tgataaattt attatggaat ttagttcatc
    34741 tgcttttata gtaagttctt tgtatttagt atttattcct gaatataaac taattatttc
    34801 atctgtatac ttaattagcc tctcagtaat ttctttatca tccagtttga gttttgctat
    34861 tatttgaata aacgttgaac tatcaatata cctatggcct aaatcaataa cttcttctat
    34921 atattttgga actaaacttg actctcctgg cgttatagct atcttaatat ttctatattc
    34981 ttcattggga ttgtatggta taaaattcaa atgatttatt cctatattgt ataaattaga
    35041 tattgtttcc aatacagtat gcttattatc gtttactact aatacatcta ttccttctgg
    35101 caatgaaaat aacttatata tatccttttg ctttatgctc ctatttatag ttattatctt
    35161 tgagacatct tccacatatt tttttatttt taatactcta tcatctatca ttaccaatat
    35221 tatgtcatct tttattaatt ccttctctgt tattttattt aaataataat tttttatcat
    35281 tactttatct ttaaagatca tttctagatt tttatttaag aaacattcta atttagacaa
    35341 cccatcagtt actactgcta ttgttttcat ttgattactc cttttaaaat atactctact
    35401 cttttataat atacatttga ttttcacttt aataacacaa aaaattgtac atttagtata
    35461 ctaacatatt cctttattag ttttatgtcc ataaatatca tagttgcaat atctatcata
    35521 tttcttaaac ttctataaag caaaaattgt gccaaattaa taattttctt aattttattc
    35581 taaagttcct caatagtatt tacagactta gcacttatag cttataagtc tttatattat
    35641 taatctattt ttataggtta attgtacaaa tcatatatca cttttaccta ttaaatgcat
    35701 tataaaaaat tgtatttata aacactattt actactcttc taagtacaaa cagtatctac
    35761 atactataac ctttcaccat tagattctat tacttgttta taccagtcaa aactcttttt
    35821 cttaatccta gacaagtctc cacttccatc atcatactta tccacgtata taaacccata
    35881 tctcttggcc atctcaccag tagatgcact tactaaatct atacatcccc atggagtata
    35941 tccaaataaa tcaacgccat cttctacagc ttccttcatt tgctctatat gtgcttttaa
    36001 atactctata cgatagtcat catttatcac tccattagat tcttttttat cataagctcc
    36061 taaaccattt tcaactacca tcataggtat tctatatcta tcataaattt catttagtgc
    36121 atatctaagc ccttcagggt ctatttgcca tccccattca ctagacttaa gatatggatt
    36181 tttcactcca cctaatatgt tgccacttgt attatcttct ttagaatcac tacttacaca
    36241 tgtagacata taataactaa atgtataaaa atctacacaa ccttctttta aaatttctaa
    36301 atctcctttt tccattttta tttcaatatt attttctcca aaatatctat tcatataagt
    36361 tggatattcg ccacgcactt gtacatctcc acagaaataa ttcattactc tactatctct
    36421 ttgagtttta actgcatcat ctggattaca agtataagga tacattgttg caaatatttg
    36481 catacatcca acttgataat ttgaatctat ttcatgagct aacttaacag ccttagcact
    36541 tgctacaaat tgatgatgta acccttgaaa acgaagttga ggtatatcta cttggtttat
    36601 aaaatctgta gtcccttcat ttaatattcc ttgtgataag tagcctccca taggcattgt
    36661 agctgaattt atttcattaa atgtcagcca atattttact tttcctttat atcttttaaa
    36721 tattgtttca cagtaattca tatacaagtc tattacatct cttgaaaccc atccattgta
    36781 tttctctgtt attgcaaatg gtatttcata gtgagatatt gttattaatg gttctatatt
    36841 atattttaaa cattcatcta tcacattttc ataaaattta agtcctgctt catttggttc
    36901 tttctccatt ccagttggaa atattctagt ccatgctatt gaaaatctaa ataccttaaa
    36961 tcccatttct gcaaataaag ctatatcttc tttgtatctg tgataaaaat ctattgcttc
    37021 atgacttgga taaaatgttc ctttttctat aactctcgtt attcttttac ttactttttg
    37081 agaacctcca gtacacatat ctgaacaaga aggacctttc ccatctaaat tccatgctcc
    37141 ttcacattga tttgctgctg ttgctccacc ccataaaaaa tcttttctaa gtgccataat
    37201 tctacctcca ataatttatt tatagtaact ttattttaaa ataaaaatac atatatataa
    37261 ttcttaatta atagaactct cttttacgct aaaacagtta tcaatttctg actattattt
    37321 acatgctttt gtgtattttc tacaatatcc acataatcag aataattagt aacaactact
    37381 ggtgtttcta agcaatatcc ttcttttgaa atactttcta tgtcaaaagt tattaatgtc
    37441 tgaccttttt taatcttatc cccttgatta acaaaagatt tgaaatattt accttccaac
    37501 tgaatagtat ttaaccctat atgtatcaat agttcacaac cattatcaga tataattcct
    37561 attgcatgtt ttgttggaaa taaagtcatt acagtcccat caaatggtgc cactactttt
    37621 ccttctttag gtgttattgc tacacctttt cccaatatac ctgttgaaaa cgctgcatct
    37681 tgtatctgct ccaaagcctt tacttcacct tttattgggc tatatactac ttctcttttg
    37741 tttttttcta taacgccttg cccattatca tctttagaaa tttctactat agcatcatct
    37801 ttccaaaaaa ataatgttaa aacaaatcct actacaatag ctactattat tgctattata
    37861 gttgcataaa cacctttcat atctccagtt gttggattta tataattagg tataccaaat
    37921 attccaagtc cacccattac ataacttttt acattcatta tccccattac agctcctgcc
    37981 acagcaccac ctacacaact atatatgaat ggtgtttttc ttggtaaagt tattccatat
    38041 attgcaggct ctgttacacc acatatccct gaaataactg caggtataca cagctctttt
    38101 atcttcttat cttttaattt aaagtacatt gctgctacta ctgctgtttg tgcaaaagat
    38161 ggtacaaata ctcccactaa tattgtgtca aaccccatag tcataaggtt tgacatagct
    38221 attggtacta atccccaatg tagaccaaat attacaagca cttgccacaa taaaccagtt
    38281 aatgcaccac ataaaattgg tgaaaagtta aataaagcta aaaatccatt tccaactata
    38341 ttagaagcat aggtagctat aggccctata accataaatc ccaatggtaa agaaactaat
    38401 aaaactgcca ttggtacaaa gaaattcttt actacatctg gtattatttt agcaaacact
    38461 ttctccaact tagctgctaa taatactatt aaaattactg gtactactgt tgaagtgtag
    38521 ttcattgcta ttactggtaa tcctaagaaa gttatatata ctggtgattc aaatattgtt
    38581 ccagtaaata gattgtataa aggttgacct gtagatgcta gagtatctaa ttgaatacct
    38641 gaatagcata aaactgcacc tataagcatt ccaataaatg gttttaattt gaatttctta
    38701 gcagctgtat atccaagaat tattggcata tactggaaca ttgcatctcc aattgcattt
    38761 aacataatat atgcaccatc agtttctcca tacaaaccca atgctgtaaa tactgcatta
    38821 aatcctttta tcataccagc agcacacaga actcctaata ttggttgaaa aactcctgat
    38881 attatgtcta taaacttatt aagtaatcct tttggcgcat ctgagtctga attatctatt
    38941 gtatctcctc ctaatccagc caaatcgcac acaacttcat aaacatctgg aacatgatta
    39001 ccaataacta cttggtattg acctgcactt ttcattacag taactatacc atccattttt
    39061 tttagcacat catcatttgc tattgactca tcttttaatt taaaacgtag cctcgttata
    39121 cagtgagata aactgctaat attatccttt cctcctacat tcgcaatgat ttcttttgat
    39181 agttgttcat actttctcat tttttctcct ccattttctt tttgaaatat gaaggtttgg
    39241 ccaacttaat gtcaccaccc aatatatata aacctaatta ttaggattta tatccgactt
    39301 aatactttta cttaccactc tttcaacgtg aatagttaag tataattttt cctcatctgt
    39361 taaatcgtag ttgtattttt tatgtataaa ttcatccaac ttttctatga atttataagc
    39421 atttttatat ttaactttta ttacatttaa taaatcatct tcattatttt catgtaaatt
    39481 attactaaca attcgttctg caaaatactt taagtgagta ataaatctat gataataaac
    39541 tgaatcttca tcaaatacta ttctgtaatg gtatttaacc atatttgaaa tttcttgtat
    39601 tatttgtgta atctcatata tagtttttat actttgatcc atttgtgcat taactatatg
    39661 cagtgctata aatccagctt catcttctga tagtctaatt tcaaattttt tttctatata
    39721 atccaatgcc tctaacccta ttttaaattc actcttataa aatcttttta tatcccaaag
    39781 taatacattt tttacactta ctccttcacg agctctttct attgcagtat acatatggtc
    39841 tgttaaagaa atataaatac tatcatttaa tcttttccct agtttttctt tagcatataa
    39901 aattatttca tctgataatt ccatatatct tataggaata tctgctatta gttctttaaa
    39961 tttatcagat atatttgggt ctgaaagttt gaatatctta tcaattttat cctttgatat
    40021 atgctctcct gtacgttttt tataagctaa acctctcccc ataactacta tttccttatg
    40081 attttcatca gttgtaataa ttacattatt attgagtatt ttttcaataa tcattgtgat
    40141 gcccccttta ttagcattaa gttaaaataa atctttttta attatttaga atatttaaaa
    40201 tgtaatttat tacataaaaa aaaacctatt tattacacat taaatagact cccatgagtc
    40261 ctgagtaata aataggttta gcttgtattt aaacaatcac tatccgtttg tattttattt
    40321 taattattat ttattatatg ttagcaaatt tattttttag tgtcaacttt ttctcaatat
    40381 tttaatttta aataaattat ataaattgaa tcttcaaagt actattttta tcaataaaca
    40441 aaataaaaaa ggtattactt tctttaagta atacccattt atttctaatt taagtggttt
    40501 ttcttaatct catcaagtag tccatcttct aaataaattt ctaatgcaca tcctaccatt
    40561 gcctttactg ctttgtgaag tttatcataa gcttcctcag aatttgcata atttagaaat
    40621 tctttttcat gaacatatac cattggactt atatctaagg ctatttctgt atacatagta
    40681 gggcaaactt gacttacatt acctatatct gtagaaccta ctggaccatc tttaccttct
    40741 aatatatctg ttatacctgc ctcaatcaaa ttattctttg ttatcttttg aagtactttg
    40801 ttatttatta tgttgtcaaa agaattttca aagtatctat aagaaagctt agctcctgtc
    40861 attaactctg caccttttgc acagtttata acttttttag ttacttcatc taaataactt
    40921 ctttcagcag ctcttacgaa aaacttacaa gctgcttttt ctggaactat atttggagcc
    40981 tctcctccat ttgaaactat accatgtatt ctaacatctg attttacatg ttgtcttagt
    41041 gcatttatcc cagcaaaagt aagttgtact gcatctagtg cattgactcc catatgaggg
    41101 tgactagctg catgtgctga taccccttca aaactaaact ctaatgaatc tatcgccaaa
    41161 gccttacagt ttatattatt aaaagcttct atatgcattt gataacttac atcaacatca
    41221 tcaaaagctc ctgccttaac taaatcacat tttcctcctg ttgtttcttc agctggtgtt
    41281 cctattagta caatttttcc tttaaaattt tcttttaact tacttaatac tatacaagct
    41341 cctagtgtag atgctgcaat ccagttatgt ccacaagcat gagctggttc tttattttct
    41401 ccatatccag gaagtgcatc atactcagct aaaaatgcta tggtaggtcc atcattatct
    41461 cctaattcag ctctaaaagc agtctccata ttacaatatg gataaactgt atcaaatcca
    41521 tattccttca ttttttctac taagtatttt gatgatatgt attcttcttc ccctaattca
    41581 ggattgttaa atataaaatt acaaacttct tcaatttctt ttccaagttc attctctatc
    41641 tcataagctc tcttctttat actctctatc ataatttacc tcctactaga atcctagata
    41701 taatcctatt gtcaaaaatg ccattacaag aattgttaat actaccatta aaggaattac
    41761 gaatctaacc cactttgacc aaggaatatt ggctactgct agtgctccca ttaaaacacc
    41821 tgatgtaggt gttatcaagt tagttacacc tataccaaat tgataagctg taactatcat
    41881 ttgtcttgat acatctacta agtcgccaag tggtgcaagt acaggtattg taagtgatgc
    41941 aagccctgat gctgatggta ttaaaaatgc tattaatcct tctattaaga aagttaaatt
    42001 tataaatata gtttttggta atccatttaa taaatttgct gcagaattta aaattgtatc
    42061 tattataaat ccattttctg ctattattac aattccacgt gcaaatgcta tacacaatcc
    42121 tgctgtcatt aaatctcctg ctccacttat aaatgaatta actatatcat cttgtttaag
    42181 acctgatgct atacctgata ttatacctat agctgtaaat atcattgcaa tttcagttat
    42241 ataccatccc aactttaaaa ctccatatat cataatcgcc atacctattg tgaaaattgc
    42301 tattaccatg gcttctcttt ttgtaaattc ctttatattt gtatcactat taaccattga
    42361 ttcttgattt agtgatatat cgtgtactaa agatttttta gggtcttttt taactttatt
    42421 ggcatacatc ataacaaatc ctatagatat agccatatat attataaaca taacaactct
    42481 aaactctatt ccagaacctg gtgctatttg tgataatgct tgtgctattc ctacagagaa
    42541 aggattcgta gtagctgctc caaatccagc tgttgctcct ataaatactg tggccactgc
    42601 tgtcaatgaa tcatatccca tactggtcat aagtggaata aatatcatat aaaaaggtag
    42661 tgtctcttca ctcattccaa acgtcgtccc acctagtcca aatagaatca ttgaaatcgg
    42721 tattattaaa aattgaaact tcttcatttt atttacagta tgagctataa cagcttctat
    42781 agctcctgtt ttattaacta tcccaaaagc tcctccaact attaaaacaa atccaactac
    42841 ttcagcagca tctataaacc cgtttatagg tgctgtaaat acatcagtaa ttccttgtgg
    42901 atttgatggt gctttttcat aagttccagc tacaacaact gaccttccgt ctatatctac
    42961 tctttcaaaa tttccacttg gcactatcca agtaagaatt gccattaaca ctattagcaa
    43021 aaatattatt gtaagtgtct gtggcatagt aaactttttc ttttttaaac tgctcatttt
    43081 taatctcccc actttttaaa ttaaatattt tattattgca tccctttatc ctattttgta
    43141 tagatgtaaa aaagctcaac atggatacat gttgagctaa atgtgctaaa tacaacatac
    43201 ctgcatcttc cctttatctt gtttaagata gctctccatc aaaattcggg ataattttga
    43261 tgacagtact gtacctatta aatacagtcc cagcactagc ttcggacact agcacttcgg
    43321 cacttactcc ttttatattg cctcattggt ttccaacctc ttcaatatta atcttagtaa
    43381 atgcaacctc tacctcaatc tgtttgttag atgaggtaat tcttattaat ttttattaat
    43441 tcaaagtaat tgtagcataa tatgtcgaga ttgtaaatat tttcagtata ataattctta
    43501 ctttatgatt tcattccatt tttcacaaat aattttttct ccatataatt taaatactgt
    43561 agtatatttt aaatacacac aaactttcca caaattttaa attattataa tacctttata
    43621 ataaaaaata aaagctatgt aaacttatca atttacacag ctttatatac attaatctac
    43681 tccaagtaca gctttaagct ttgatgctat tgtaataact cgtggaccaa atataacttg
    43741 aactgagttt ttctttttaa caactccact tgcacccaat tcatttaccc agaagccatc
    43801 ttcatagaat ccatcaattg aatttagctc taccctaagt ctagttgcac agtttgtaac
    43861 attttttata ttattacttc ctcctaaacc ttctattata gaatgtacaa gctttgtatc
    43921 tttatcatct acttcctctt ctactgcatt gttgtatgct gttttaccag catctacaga
    43981 ttgtctttct ctatattctt tcttactata aagcttaact tcttctgaac ttcttcctgg
    44041 tgtttttata tcaaacttct tgattgccca tgtaaatatt atataagtaa ctataaaaaa
    44101 tattggtatc aaaaataata atggtaacgc atccactttt tgtggttgta ataaattagg
    44161 aaacatattt ctaaatgatt ctcccattat agaaacatta aacatttctg taaatacata
    44221 cgtcaatcct gccagtggta catatgctac aaaatacaat aatggtgcaa caaataggaa
    44281 tgtatactct attggttccg ttacccctac caacatggct gttactattg ctggtatcaa
    44341 tatacttatt gcttttttct tattagcatt atcagccaac ttatacatag ctaaagctgc
    44401 tgctggtaat attgcaaaat gtattaataa acgacctgtt gtgaagttac gagttatata
    44461 agatagtgta tctgggtctc ccatttgagc taatcttata ttattaacac cctcgtaaat
    44521 tgctccacct acttccattg ttccacctac tcttgtatat tctataggga atgcaattaa
    44581 gtgatgtata ccaaatggta ataatgcttt atccattgct ccaaaagtaa atgttcctaa
    44641 taatccacta gaacctatcc aattaccaag tttagataat gccaacccta ctggtggcca
    44701 tataaagtac attgctgtac ccaatactat tgatgctata aacatcataa tcattgaaaa
    44761 tcttggacct gagaaaaaag ataatgcact aggaagttct atgtcaacaa atttgttatg
    44821 tactgcacta gctacaaatc cagctattat agctccaaat atactacaat catatgaaaa
    44881 gattcctaag aaagaaccat ataaagaact tcgactatat gcttctgcct ctcctaatcc
    44941 agatgccata aacgcttctg ctgtaactgt atctgaattt ataccatttg ctgctaacaa
    45001 tgtacttata actgtgtgca ttcctataaa taaaacaatt cctcctaatg caccccatcc
    45061 tttttccttt ttagataatc caaaggctat acctgtaaca aagaatattg gtaagtatct
    45121 cattaccata aatcctaaat cgtttattac tttaaagaat acatttataa atgacccttc
    45181 ttttattagt ccgctaagcc ctaatgcaga aacgttttcc atatttgcaa aagctgaccc
    45241 tagtcctata aatactcctg ctacaacaag taatataaca ggtatcatca ttgctccaga
    45301 aagtacttgt aatttatcct tcatagtatt atttcctcca tttttattga aatgccaggc
    45361 tatataatgc atatatatat acttctattg ctttgtataa tgtatctata cttatatttt
    45421 catttgcctg atgtgaattt ggattatccc cctctattgt gggtccaaat gcaactccat
    45481 tttttaacac tcttgcataa gatgcagctc cttttgtaaa aagttcggct tcttttccaa
    45541 aaccttgttt atatgcattt gataatttat ttattaattc actatctggt tttacataca
    45601 gtaattttaa atccttatat atatcaacta ttaaattttc tttttttcca atttcattaa
    45661 ttctattagt tataaactcc caacttatac ccttaggata tctaaagtct attttcatat
    45721 tcaattcatt tttctctaag aagatactca ttatacatat tgtagaaact cccatatcta
    45781 catcttcttt atatatccct aatttttttc catgattatc atctgtaaag tatgaattta
    45841 aaatatccct tattataacg cctttatcat ttagtttttt aattttctct aaatcttttg
    45901 ctaaactaaa tgcacagtta tatgacctgt gtggatttct cgataaagcc ctttctcctg
    45961 tatatcttac taatacttta ccttcttcta gttcttctat ctctgtataa taagctaatg
    46021 attctactaa atctttctta tcattagttt taaaaacagc ctcaattttg tcacatacaa
    46081 atccatcttc tttgtttgac tttatatcaa ctaaattgtg atttctaaat ttatctttat
    46141 ctattttctt tcttagattg aagtataaaa tacctttttc tccatttact attgggaagt
    46201 ttccatctgg agaaaatgca aattttggct gttcattgac actaaagtaa tgctccatac
    46261 attcccaagt cgtctcttca gctccaccca ctatcaatct aatctttctc ttcggctttt
    46321 cattaagttc tcttaagaaa agtaatgcat ataaaatccc tatcaaaggt cctttgttgt
    46381 cattaactcc tctaccataa agatagttat ctttttgaca tactttaaaa ggtttactct
    46441 tccaaagttc tttattatat attggcacca catctatatg attaagcacc cctactactt
    46501 cttctccttc tccatattct atatgaattg catatccatc aaagtctttt acaacaaaat
    46561 ctttgtcttt tgctatttga ataagcttat cgaaggcatt tcttatttct atcccaaatg
    46621 gttgatttat atctgttgta ctttcatctc taattgaagg tatttcaact aaacttctaa
    46681 tatcatttat gatattttct tgattggatt ttaaataatc aataattagt tctttgttta
    46741 ctatcatctt aattatttat caccccttta tattaatttt acaaagcatt tcagttcata
    46801 tctacattat aaattttgaa tattattttt tcaatatttc aaatacttct ctaagttacc
    46861 ataatgatac ggtaaacaat caacagatta tgacaaaatc atactatacc tattactaat
    46921 attttaactt atctattacg tcttttttaa tgtaagttta ttccaaaaat acataaaaaa
    46981 agatgtagcc ttagctacac cttcttatat ctattcaact tccattatta tgctattaat
    47041 atctacattt ccaggttcta tcctatcttt tactgttctc ttaacagaat cagcatcatc
    47101 cattataact acaggagtta ttatatttaa atcatttgac gttataaaat ctaaatctac
    47161 ttccattatt aaatctttag cttttacctt atctcctgcc tttaccttaa catcaaacgg
    47221 ctttccttct agtgacattg tctctaaacc tacatgtatt aatatatttg tattatcatt
    47281 actcttaatc attattgcat gctttgtatc aaatacattg actatttcac catcaacagg
    47341 agaatataac ttaccttcac ttggtattat tgctactcca tctcccatca tcttttgggc
    47401 aaaaactgca tctggaacat cttctaagtt cactaaattt cccttaaaag ggctatatat
    47461 tttctcaatc ttattttctt ttttcttaaa tatattaaac ataacacact ctccacatta
    47521 taaaatttta tgataatatt tttcttaaag cttttgctac accatcattt acattagtat
    47581 cagttacaaa atctgccata gattttactt catctgtagc atttcccata gcaataccaa
    47641 gacctgcaaa tttaatcatg gatatatcat tttcattatc acctatacaa ataacttctt
    47701 tgctatctat acctaatata tcacaaaatt ttttaactgc aatacctttt gaagtacctt
    47761 tactcataac ttcaaagttt atctttccag aacttactac ttctaaatct tcttgttttt
    47821 taaattcttc ctttgcttct ctcaaagaat ctacgttttc tgagaaaagt atacatttta
    47881 aaatttcatt atcagctttt tctaataaag tttcacaact tgcattttct attatttcaa
    47941 ttctgtcttc ttctttattt ttactattta ttaatctata tggatgttct tcacctattt
    48001 cattgttgga tataactatt ttacagcctt taaaatgttt gcttaaatta tacttttcac
    48061 ctattgtata gattttcttt agagcttctt tgctcaaaac cttcttatac tcatatccca
    48121 gtaatttaaa atatgtccca ttagttgata taatatatat atcatcccct aaaacacttg
    48181 taaaatattt catagcatta taaggtcttc ccgtaactaa ggctaccttg ataccctttt
    48241 ccatgacttc ttttagtgct ttaatatttt cttctgatac ttcaaatcct ttacccatca
    48301 aagtaccatc catatcagta caaattaatt tatatcgcat ttgtatcaca tcctagtaag
    48361 atttttctaa tatttcaaca aggtcttctt tctttccaaa aggtaaaaat cctaaaactg
    48421 gacagcttgt tatttgttct aattgttcct ttgttacatt aaactcagac atttttgtct
    48481 taagtcccac tctctttaaa aaactttcta tttcatttcc taaagtttta gctgcttcat
    48541 catcttccac tttttctaat tcttggttta gcattctcgc tactttggca aatttctcta
    48601 tattatcctt atagaacttt tctgcaaatt ttgggaacac tatagccaaa gcctcaccat
    48661 gaggaatatg tgtaactcct cctattattt cacttagtgg atgtggtgct gctgctccag
    48721 catttgccag tgaacttcct cctaatgtat ctgctactgc taactttgtt ctgtattcta
    48781 tattattttt atcttccatt actttaggta gatagttaat gactaattcc atagattttt
    48841 cagacattat ctctgtaaaa tcattagcat ttggatttat ataactttca aatgcatgag
    48901 taaaagcatc aaatgctgtt gatgcagtaa ttctctctgg cagagttaac ataagctctg
    48961 ggtcaactat acattctttt gagaaacaat tttgatgaaa tattgtattt ttctcgcttc
    49021 ctctcgatat tactgctgct tgtgtaactt ggcttcctgt tccagaagta gttgatacag
    49081 ctattaatgg taattctttt tcacttacct tatcgtatat tttaaatgga tttgtatatg
    49141 tactaaacat ataatcccaa tctatctttt ctaaaccaaa agtcaatgct aatacttttg
    49201 cagtatctat acttgaacct cctccaacag ctaaaacaac atctacagat tcttttttag
    49261 ctaattcaaa cccttcattt attaattcta cagttgggtt tggctctact ctatcaaagt
    49321 gtacaacttc tatacctttt tctattaaat tcaatgtaac tcttttatat aacttgtcta
    49381 atggctctac atttggagta gtaactaaca aacatttctt tccatatttt cctattattc
    49441 taggtaattc acttaaagtt ccttgtccaa aatttattct tgatggttga taaaagttaa
    49501 tcatattttt cctcccttaa attattgcct tcatattcaa attatacata tcataggatt
    49561 tttaaggcaa taccttcact gttaactctt atttactaaa gcttatggca attattagac
    49621 aatcaatgtt ttattattac catatcatta tgtaaatttt attaagtacc catgttataa
    49681 ttttataatc aatcttattg tataattgac ttaataataa aaatagagta tatacacttt
    49741 tttatgacaa atagcttaaa ggagaattaa attatgaaat atttgataga cagtgctaat
    49801 cttgatgaaa ttcaaaagtg tttaaagtat ggatttaagg gaataacagc taatccatct
    49861 atgtatctta aagagaaagt aaacttccat gaatttattg agacatgtgc taatttaaat
    49921 ccagaaatat taactgctga agtcattgga aataatttat acgaattaat taattcatgt
    49981 gataagatat taaatataaa taaaaacact ataataaaaa taaatttctc tgaagttggc
    50041 ttagaactta taaacatatt aaatagtaaa ggtgtaaaaa ctgcatgtac cttgattttc
    50101 aatgtaaatc aagcatcatt agctataaat gcaggtgctg attacctatt cccttttata
    50161 ggtagaaatg atgaaaatgg gaatgatggt ataaaaaacc ttacagaaat atgtaacctt
    50221 atatcaataa atggatataa tactaaagtt gttgcagctt ctattaaaaa tgtattccac
    50281 ttaactcaag catctctttg tggatgtgat tatgcagctg taacatatga tttatttaaa
    50341 aaagcttctt ttaaccagct tacaatagat ggtgccaata cttttgaaag agattggtcc
    50401 aaggttaata aatagtaact aagatttagg agggtttgca ttgaacaaaa gattattaga
    50461 tattgctgaa ttattactca acagctcaga ctatattact gtagatacta ttgccgaaga
    50521 attaaaagtt agtaataaaa ctattcgtaa tgaccttgtt attttagatg aatggctttt
    50581 agagtttaat ttatccctag ataaaaaaac tggttcaggg gttattattc taggtaatga
    50641 agatataaaa cttaaagtta ttagagatat caatgataaa tcaaattgta tatatgcata
    50701 ctctcctgaa gatagaaaaa ggtatattct aagtaaatta tttattaaaa attctaagtt
    50761 taggattcgt gatatttgca atgaactaca tgttagtaga gctactgtgc ataaagactt
    50821 ggtagttatt caaaattttt tgcaaaactt taaagtgact ctagttagaa aaaccaataa
    50881 tggagtatat ttagagggaa aagagaaaga tattcgaaaa gcaatttttg aacttgctac
    50941 ggttaataaa agttatactg aacttaaaga aatattattc tcgaaatcta atgaatgcaa
    51001 agatacccct tcaacaaaaa tatttaagga gctatttaac tatgattttg agaaattatc
    51061 tcaaatcact ctacacacct tacaagtaga aaaaagtaac cttagtgatg agtattatat
    51121 aaacttctta atacatatag ctatatgtat taaaagaatt tcaattaaaa gatatattaa
    51181 cttatctgaa ggttttttta gagaattatt agaacatgaa tactttgaaa aatcaaaaat
    51241 tttatgtgct aacctaagtg aagcttttga cgtagaattt attgatgaag aggtctgtta
    51301 tatccttctt catatagaag gattacttaa atcaataaag aaaactacta ctcttgaacc
    51361 tcttaaaaat gaagaaatta cagagtataa tctatctgaa gcaatagctg ttcattgggg
    51421 aagtatacta aatttaaact taaaggatga tttaatatta ataaagtctc ttgctaatca
    51481 ccttaaatca gttctacaca gaataaacta tgggtttact attacaaatc ccatattaaa
    51541 tgatataaag agaacatttc cttatactta taaggctgct aaagagtcta atactgttat
    51601 caaacagttg atgaattatg aagtcaatga tgacgaaata ggatatcttg ctttatattt
    51661 aatatcagca atagatagaa gcaaatctcc ccttaatact attttaatat gtcactgtag
    51721 tgatggtgta tctaatttat tagtccaaaa gttaagtttt gaatttaatc agattaatat
    51781 agttaaatct atacctctat cttctatttc atttacgaac tttgatgatg tggacttaat
    51841 actaaccact gctcctgtag actttgaaca taatgctgaa ctgataaata taaatgctct
    51901 tttaagtaag aatgatattt ctagacttag tacaattata aaaaaactat attctgaaaa
    51961 aaataaaata ttatcttata aataaacaaa aataggcttg tctcaacata gagataaacc
    52021 tatttttcat tgtctaaatg ataaaaatat atttgatttc atagaaaaag tgcctcatga
    52081 actaaaaaat tcattttgag acacctccat tttttatatt taagcttttt atttaagctt
    52141 ttatattttt taaactttta tatttaaagt aaattttatt atttttattg agcacaagca
    52201 ttatttatat tattaatttc ttcagtattc ttaccaaact ttgtagttac aaatcctatt
    52261 gtttttatta tgagtaataa agatatcaat gaatatatag atatcataaa attcttactt
    52321 attattccag acgctactat tataaatcca tctagcataa ataatataac tggcaacttc
    52381 agaaacttaa ttaccttatt aataagcatt gcaactaaat cagttccacc agtactacag
    52441 ccccttatta ttgctatccc tatagcagtt cctaccaata acccacctat cactcctgct
    52501 actaatagat tgtcagttat gtgagtttgt ggtattactt ttaagaataa tgggcccata
    52561 agagttatat ataatgtttt tattccaaaa cctttcccta aaaataataa tcccattact
    52621 aaaagtggca tagatattcc taatgatata ttctcaacag gcattgataa gaatgtgcta
    52681 agtacgactg ataatcctgt tatccctcct ggagctaatg tatgttctgc aaagaaaaaa
    52741 ttaactgcta atgccattaa aaatgctgat attgttatca gtaaataatc ttttatcatt
    52801 tcttttttca tttaataacc tcctatgatt atattatttc agtataaaaa atcaattaaa
    52861 ttagtctagc ttcctgtagt ttttcataaa attaatgctt ccttaaaaca ttaagaaagc
    52921 atctctttta tcatagttta ttatcttatt ctaaaccaaa ctttgtagct gctttatcta
    52981 tagcttttct acaattctta acacctgctg ttataccatc tttatccttg aatattgctg
    53041 aagataatat tacaacgtct ggattacatt ctattaatgg ctccatattg tctgctctta
    53101 gtccaccatc tattgctaat tcacacttag gattcttttc gtctatcatt tttctagctc
    53161 tttttactaa atcaacagca ctctttctcc atccccagtt atcttttcca tcagtttcat
    53221 caacaccatg aacgactatt tgcaatctat ctatatcata tatagcttca tcaacaaaac
    53281 ataatggtgt gtaacatcca attgttaatc ctactttcat accaaattct ctacagtaat
    53341 ttattatgta tgcaagagga gcacctaaaa aatgttctgc tggtattatc aacaagttac
    53401 atcctgctgc tgctattttt tctataaata atctatcaca atctcttgtg tatatatggc
    53461 attctattgg tttatctgtc actggtctta ttgcttctat tatttgatga ccacccataa
    53521 gttgcatatt ttttaagtca tgcatatctg ctgcatctga atgcacgtag tctacacctg
    53581 catccattgc ttctttaact acggctgata tatgaccgta atttacatgt gctaatcctg
    53641 ctgctatttt tatgtgtttc atatttgtat acctccatat tttactttaa ttagtattaa
    53701 atattagttt ttgatatttt tttaatattt tttaatccag tattactttt atttagcatt
    53761 acttttattg ctgtatatat ttcttttatg aacttattct gcttttgtta actgtgaact
    53821 tattttgctt tgtaaattaa tattacactg atatattttt tttcacaaca ctttgactac
    53881 tccttaaaag tacctttata ttgtggtaaa ttatagacat taattgtata taaaaagtta
    53941 gttttttacc acattcttat ggcatttatt gtctatacta actcaattga tatagctagt
    54001 tcatttaata tataatcaga ctataaatat aaatttactc gataaaaagt accattcata
    54061 acaatattaa tgaaagggag atgtaaaatg agaaactgta ttattgccca atctggaggc
    54121 cctacagctg ttatcaatgc ttctgcagtt ggtatttatg aaaaaaattt agaaactaaa
    54181 tactttgata aagtatattt tggtatcaat gggattgaag gtatattaaa caaaaatata
    54241 atagattcag actccttaaa caaagataat gtattaaatt taaagcaaac accttcgtct
    54301 ggtcttggtt cttgtagata caaactggct gattataaaa taaattctta tgattatgat
    54361 agagttataa atatactaga tgaatatgaa atagatacat tattttatat aggcggaaat
    54421 gattctatgg acacagtgat gaaattttct caatactgca aaaacactac ttgtaaaata
    54481 aaatttatag gtattcctaa aacaatagat aatgatttat taggaactga caactgtcct
    54541 ggatttggtt ctgctgcaaa gcttatttct acactaataa tggaaacata cttagatttt
    54601 tctatataca aaaataatgg tatatttata gtagaaacta tgggtagaga tactggatgg
    54661 ttagcagctt cttcttcact agctagactt aatgatactc cacttgttga tttaatatac
    54721 cttccagaaa ttgatttcta tgaagataag tttatagaag atgttagaaa aatatttaaa
    54781 gaaaaaaata aggcttatat agttgcgtca gaaggtataa gggataaata cggcaacttt
    54841 atatgcgaac aaaaaacttc tgctcatgat aaatttggtc attcccaatt aggtggagta
    54901 agcaactatc taaagaatct tatagtatca tcatcaatta caacaagagt aaaaacctta
    54961 gaacttggtg taatacaaag atgtgctatg catttagctt cagatactga tttaaaacaa
    55021 gcagctgaag ttggtgcata tgggatagaa ctatctaaaa aaggatatac aggagttatg
    55081 gctatcatta atagaacatg caataatcct tataagtatg aagttagtca tactgatatt
    55141 tctaaaatag ctaacaagac aaaatatttt cctaaagagt ggataaatga agaatgtaat
    55201 tatataactg atgaagccct aaattatcta tatccactta ttcaaggtaa accagatatc
    55261 aaatataaaa atggactacc ttatttttca attatttact aggaaattat tataatttga
    55321 tatacactca attctatcgc tcttaaatac ctaagtggct gttaaataaa attataaatt
    55381 gttatatgac agtcacttaa tcgtttaatt atttaactgt ttagtttttt aattttttaa
    55441 tcttttttaa tactaatacg atttgttgta ataatatctc acacccattt tcattttgga
    55501 tagattataa ttagttagac aataaatatt ttgtcatgat attattatat atagtgatac
    55561 aaatagaaat acttttatca tggctagaaa aacaaagatt acacaaagga tttaaaaatc
    55621 aaacaacttc aattaaaata aatggttaaa ataacttcta aatcaactac tataaacaat
    55681 aaaagaacat aaagtagtta agtctacaat tataaaatta gtaagactat aataacttaa
    55741 ataaaattaa agttaacaaa gactattaaa gttagcaaat attcctctac ctaaataatt
    55801 gctttttata aagagttatt tattttaatt gaatttaatt gaaatacgat tttaactaga
    55861 acaatagatt aatgtaatat ttaatataaa ataattataa taattgaaaa ggaagtaatc
    55921 tatgatgaaa atagcagtca taacaaccga atttttaaaa gagtttgtag ataattctat
    55981 aaaaaagtta aatattaatg cagaaattga aatatatatc tatagagatt tttctcatgt
    56041 tggagattta tatttagaaa tagaagatag gtttgatgga tttgcagtta gtggccccat
    56101 ccccaaaaaa gctattacaa aaaaggctgg aactattaaa aaacctctag ttgattttgg
    56161 tactgactta caaagttact atgatatgtt tctaaagctt atctttaaat ataatacatt
    56221 agatttttct agagcgtact ttgacttaac agactgggca gataatccaa aagatatatc
    56281 atattattta tcaaatggca catttggaga cttaatggat agtattgatg aaaatgcatc
    56341 taaaatgagt ttatctgaca tagcaaaact tgaaaaaaag attgcaaaaa aacatattaa
    56401 tctatggaat gaagggaaaa tcgatttttc tactactcgt tttagtagta ttgttcctat
    56461 gttacaaaat gcaggagtaa actttcattt catataccct gatatagatt tattaaaaaa
    56521 tacttttaat aacttaataa aagatattca tttacataga atgaagggaa atcaaccaag
    56581 tgtaatatat ataacagtta aagatacaaa ttatgatgac tctactctag accaagttta
    56641 taaaatatta aatagtatta aaaaagataa attaaccgat tttataattg aaaaaaatag
    56701 taattctttt aaaattttca ctaaatgtga aacgattaaa tcattaacta aaaattttac
    56761 tgcatgttat ataaaagaat ctattgaaaa tagtacaaat attaaagtat gtgttggata
    56821 tggaattggt aatgatataa agcaagctat gtctagtgcc ataagtgcta ataaggaatc
    56881 taaaataaat catttaaatt acagtttctt agtcaacgaa aattatgaga caataggacc
    56941 tttactgtgt gataagttac ttactgtttc aaataaagta actccacata taaatacaat
    57001 tgccaaaagt tctaaattgt ctactcttac tattcaaaag attatatctg ttactgaaat
    57061 tctacaaaga gatgaagtta ctcctaagga attatcttca tgtttaaatg taacagttag
    57121 aactgctaat agatttctaa gtagtctctt aaagtctgga aacgctacta tattatatga
    57181 aaaacaaaac tcaacaaaag gtagacctga aagagtatac aaattattta tatcagaaaa
    57241 taaatattaa aatccaaaat gcataaaaat attttatcat ctaatattaa gtaaaattat
    57301 atatgacttt ctaaaaggta tatctttgca tctttttaag atataccttt tataatattt
    57361 aataatattt tatgttatta tctactaaaa ttattttcta accattttac tgcacaatga
    57421 gcaaataaag ctgaaccata aactaatccc tcttcattaa aaattgcttt aggattatgc
    57481 ataccaaaat tatatccctg actaatactt cccattccaa tccataaaaa tactcctgga
    57541 acccttagta aaatctctga aaaatcttct gaacccattg cagatggaaa gtctattaca
    57601 ttttcatttc ctataacttc tttaacataa gatacaatct catctcctac atccaaatca
    57661 tttataactg caggagtccc cattttaaat tccactttag catttcctcc aaatgattta
    57721 gctattcctg tagatatttc aataattcta ttttttaatt tttctctaac agattggtca
    57781 aatgttctta aagtacctga catagatgct ttatttggta tagcatttgg tacgcttcct
    57841 gcattaaaca taccaatagt taaaactaca ggctctttct gaggtttttc tcttgaaagt
    57901 atagtgttta atgctatttg tatatgtgac attatgttta ttgggtccac tccaaattca
    57961 gggcttgctc catgagttgc ttttccttct actgttattt caaatatatc tgcagatgcc
    58021 atagctggac cacgtgaata agctacttga cctgtgttaa gaaatttacc caccatactg
    58081 tgaaatgcca tagctgcatc gacctgagga ttttctaata taccatcatc aatcattgat
    58141 tttgcacctc tggtagtctc ttcatctggt tcaaatacaa actttactct acctttaagt
    58201 aagtgcttat tatccataag tattttagct gctcctaaga gcattgctgt gtgattatcg
    58261 tgtccacaac tatgagatgc atctttattc ttacttttaa aaggtaaatc cgtctcctcc
    58321 ataataggaa gtgcatccat atctgccctt aataataagg tctttccacc atttttactg
    58381 tctaaaacag ctactattcc attattagac gtcaatttcg gagaatatcc cattttttct
    58441 aaagtactgg taacaaaggt tactgttttc ggtagattgc aaccaatttc gggattttca
    58501 tgtaaatatc ttctccatct aataatttcc ccttctattg actgcgcttc tttaaaaaaa
    58561 tttttcatat tccttcctcc taatcagata gtcagcaaaa tctcactttc ttttagtaat
    58621 gaaatgatgc tgatataaaa attataattt tatatattaa atttcatgtc tttttaagga
    58681 gttggaatat catctggaat aggacattta tatgttttac cattcataga tttatcaaat
    58741 tcttcttttg cctttttagc tatctctgga ttatttaata ttttagctcc aaacatagcc
    58801 ataatctttg atgcatatat catacctttt tctcctatag aacttcctgc acaagatgta
    58861 aactgccagc tatgacctgg tgctcctaga ttcgtacacc ctgtaaaaaa gtatgctgtc
    58921 ggaactatat gcattacatc tccaatatca gtagagccat aactattaaa gtttgtaaca
    58981 tttcctccac cataatatag atgagttcct tctggcaaat tatatttact tatcatagcc
    59041 ttatattgtt gtgggctttg tctatctaat tcagctgcaa aatccaattc ctctcgagtc
    59101 caaggctctt gtttaatctc attcatacat tctgatacaa gatttgctaa aacatgattg
    59161 tttaaagtat tataacaccc tcctaaaaac tctacttcaa cttcagtttc agtcatcatt
    59221 gcagctcctt ttgctacctt tacaagtctt tcatatgtac tttctacagc ttctctactt
    59281 aaagctcttg tataatacca tactgatgct ttatcaggaa ctatgttggg tgctactcct
    59341 ccatctgtaa ttgtataatg aatccttaca tcagaagtaa catgttctct caaataatta
    59401 gctcccacat tagtaagttc cacagcatct agtgcacttc ttccattttg agggtctgag
    59461 cctgcatgtg cagtctttcc cttaaagtga aactttacag agttaacagc tgttgaagta
    59521 ccaattgtag cttcactaat tttatttgga tgaaaattta tagctaagtc taacccttca
    59581 aacgctccac ctctagccat aaatccttta cctgttaatt gttcttctcc tggacatgca
    59641 taaaatacta tagtcccact taagttattt tctatcatct ctcttttaag ccctactact
    59701 gcccctacat gtgctgtaca taataaatta tgcccacatc catgaccata tgcacccaat
    59761 tcaaatgctt cttgttttgt tgaaactttt tgatttaaac caggtaatgc atcaaattct
    59821 cccataaaac ctataactgg tttacctgaa ccaaaactag ctctaatagc cgtggctaaa
    59881 cctcctacac ctacttccac atcaaatcct tcacttctta gaaagtttgc tgtattttca
    59941 catgctttaa attctttaaa tgctttttct ggattttccc atattttctt acttaaatca
    60001 tcaagtttga atcttttttt atctatctca tttatagata tacttcctat cattttctat
    60061 tcctcctata aatctattta ctatcaacca aatataattg gacctattat actagcaaat
    60121 gccactgatg caatggttac agtaacaaat cctcctataa tcatctttgg taataaataa
    60181 tctgttgccc tttgtttttc ttcttcagtt ccttccatag catcaatacc ttcattggaa
    60241 attatctgtg ttgcaggata agctagcata caagttacac caattgcagc tgacattctt
    60301 ggagaatatc ccaatagttt tcctacaatt gcagtaaata gtattataaa tacagagcct
    60361 acaacaagta ttcctatagt tggaataatc atcccaaaca atgctgttgg agttacctta
    60421 gcaagtcctc caggtaacat caacatacaa ccaaacatta taattccatt agattgagat
    60481 ttatctaaaa ttcctttttc taaaaaacct attcgtgtaa aaatcaaccc tactaacaaa
    60541 catgctatgt tgggatttaa ataatagttt gcaggggtaa atcctggtat taaagtagcc
    60601 aatcctacaa aattaccaat catagcaaca aatgaaagct ttgcaagata tactaaagta
    60661 gatgattttt tacctaaatc ttctttaaaa attctcatac ttggaagttt aaaattactt
    60721 tttgactcat ctttcaaaaa atccccgtta tctcttttaa attttaattc tttatttata
    60781 caatatgtag ttatcggaat gccaaagaac ttttgaaatg ataatactag aattacaaat
    60841 gcagcaagtt caggtctatt tgcagcttct gcaacagttg ttacgataat accagcaaca
    60901 gtagaacccg ctattggagg tgctgctatt aaagcatact ctctaccaaa taacatacta
    60961 cccactgtta aacagccaac ccctattcct acaataccta ctaatgcaat tacaaccgtt
    61021 ttccattcat gacataagtc ttcaagattt ataagtgttc ctaagttagt tattaataaa
    61081 gctataccaa agttgctcat tgcagctaat atccctgatg ctcctgtaat gtcttttggt
    61141 aaaatatttg tccaaaatcc tagtaaaaat ataatacata caaataacaa tgttgaaatt
    61201 gcaccttttg ttttcttaga taaaaactca ctaaaagcat aaatcattac acatacagta
    61261 aagcaagtaa gtggattcca aaaattttcc attgttgttt cctcctaaat ataataattt
    61321 tttatcaata aaacaataat tataaaatgt atattatgca gttattctta actacatatg
    61381 gtatagcttg atattttttg atgatattat atacgtccta aattattcct aaaatatcta
    61441 aataaatata actagagtta actctctaaa tattaactat agttatcaac ttactagatt
    61501 tctttttgat ttttactttc tacataattt tacgagttag aattgtactt caactattct
    61561 ctatattagt atacatgttg gaagacttat atttatttaa atattagata tagaatatat
    61621 ttttataaac gtcttaaatt attcttaaaa tatctaaaac gaaataataa tatacccatt
    61681 aaaccaatat tatcagaata tacttcactt taagtcttct ttatatagaa attttatcta
    61741 ttagtgcatc ccctcccttg taaattagac tatttctaag cgaatttaga tatcttattc
    61801 ctagagacta ttactaaaaa caaaaattat ttattatatt aaatcaatga taagccatac
    61861 tttaaaattc tttctataca agtataattt atcatataat gacatctata cctttatctt
    61921 tatagtagtc ctaaattatt cttaaattga caataaaaat aatttcacta tttataa
//
LOCUS       DAMKMI010000007        58860 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMKMI010000007 DAMKMI010000000
VERSION     DAMKMI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.007"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            199..1275
                     /locus_tag="PC429_000418"
     CDS             199..1275
                     /locus_tag="PC429_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3845942.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1378..2220)
                     /locus_tag="PC429_000419"
     CDS             complement(1378..2220)
                     /locus_tag="PC429_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF3845943.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEIGSVMGTYTSKGAIL
                     ITAI"
     gene            2404..2976
                     /locus_tag="PC429_000420"
     CDS             2404..2976
                     /locus_tag="PC429_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3845944.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3139..3411)
                     /locus_tag="PC429_000421"
     CDS             complement(3139..3411)
                     /locus_tag="PC429_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845945.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3525..4397)
                     /locus_tag="PC429_000422"
     CDS             complement(3525..4397)
                     /locus_tag="PC429_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3845946.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4693..7800)
                     /locus_tag="PC429_000423"
     CDS             complement(4693..7800)
                     /locus_tag="PC429_000423"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF3845947.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGAKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7893..8125)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8300..8827)
                     /locus_tag="PC429_000424"
     CDS             complement(8300..8827)
                     /locus_tag="PC429_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF3845948.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8862..9647)
                     /locus_tag="PC429_000425"
     CDS             complement(8862..9647)
                     /locus_tag="PC429_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF3845949.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYERAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9657..9914)
                     /locus_tag="PC429_000426"
     CDS             complement(9657..9914)
                     /locus_tag="PC429_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF3845950.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9933..10391)
                     /locus_tag="PC429_000427"
     CDS             complement(9933..10391)
                     /locus_tag="PC429_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF3845951.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10431..11135)
                     /locus_tag="PC429_000428"
     CDS             complement(10431..11135)
                     /locus_tag="PC429_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF3845952.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11145..11780)
                     /locus_tag="PC429_000429"
     CDS             complement(11145..11780)
                     /locus_tag="PC429_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845953.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVNTLVLKNEKVDAK
                     IHVKGSIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IIVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12161..13201
                     /locus_tag="PC429_000430"
     CDS             12161..13201
                     /locus_tag="PC429_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3845954.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13664..15019)
                     /locus_tag="PC429_000431"
     CDS             complement(13664..15019)
                     /locus_tag="PC429_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF3845955.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15045..15314)
                     /locus_tag="PC429_000432"
     CDS             complement(15045..15314)
                     /locus_tag="PC429_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF3845956.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15604..17574)
                     /locus_tag="PC429_000433"
     CDS             complement(15604..17574)
                     /locus_tag="PC429_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845957.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYNIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGEDFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENNNNENDNSD
                     EKQPNTPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLS
                     SKSVFGTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIF
                     LFCILTPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17757..19232)
                     /gene="spoIVA"
                     /locus_tag="PC429_000434"
     CDS             complement(17757..19232)
                     /gene="spoIVA"
                     /locus_tag="PC429_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF3845958.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19404..20699)
                     /locus_tag="PC429_000435"
     CDS             complement(19404..20699)
                     /locus_tag="PC429_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3845959.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSVINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASVA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENATKVS"
     gene            complement(20883..22292)
                     /locus_tag="PC429_000436"
     CDS             complement(20883..22292)
                     /locus_tag="PC429_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3845960.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(22344..23483)
                     /locus_tag="PC429_000437"
     CDS             complement(22344..23483)
                     /locus_tag="PC429_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3845961.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVAGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23802..24452
                     /locus_tag="PC429_000438"
     CDS             23802..24452
                     /locus_tag="PC429_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3845962.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAE"
     gene            complement(24609..25628)
                     /locus_tag="PC429_000439"
     CDS             complement(24609..25628)
                     /locus_tag="PC429_000439"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF3845963.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNIIISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25659..26273)
                     /gene="plsY"
                     /locus_tag="PC429_000440"
     CDS             complement(25659..26273)
                     /gene="plsY"
                     /locus_tag="PC429_000440"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF3845964.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26308..27627)
                     /gene="der"
                     /locus_tag="PC429_000441"
     CDS             complement(26308..27627)
                     /gene="der"
                     /locus_tag="PC429_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF3845965.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27661..28989)
                     /locus_tag="PC429_000442"
     CDS             complement(27661..28989)
                     /locus_tag="PC429_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF3845966.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEMGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPENILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29227..29427)
                     /locus_tag="PC429_000443"
     CDS             complement(29227..29427)
                     /locus_tag="PC429_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845967.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29429..30361)
                     /locus_tag="PC429_000444"
     CDS             complement(29429..30361)
                     /locus_tag="PC429_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF3845968.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30406..31338)
                     /locus_tag="PC429_000445"
     CDS             complement(30406..31338)
                     /locus_tag="PC429_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF3845969.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31393..32682)
                     /locus_tag="PC429_000446"
     CDS             complement(31393..32682)
                     /locus_tag="PC429_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3845970.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32711..33403)
                     /locus_tag="PC429_000447"
     CDS             complement(32711..33403)
                     /locus_tag="PC429_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3845971.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33418..34179)
                     /gene="pgeF"
                     /locus_tag="PC429_000448"
     CDS             complement(33418..34179)
                     /gene="pgeF"
                     /locus_tag="PC429_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF3845972.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34176..34637)
                     /gene="nrdR"
                     /locus_tag="PC429_000449"
     CDS             complement(34176..34637)
                     /gene="nrdR"
                     /locus_tag="PC429_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF3845973.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34659..34922)
                     /locus_tag="PC429_000450"
     CDS             complement(34659..34922)
                     /locus_tag="PC429_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF3845974.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35026..35799)
                     /gene="sigG"
                     /locus_tag="PC429_000451"
     CDS             complement(35026..35799)
                     /gene="sigG"
                     /locus_tag="PC429_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF3845975.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35891..36613)
                     /gene="sigE"
                     /locus_tag="PC429_000452"
     CDS             complement(35891..36613)
                     /gene="sigE"
                     /locus_tag="PC429_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF3845976.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36638..37510)
                     /locus_tag="PC429_000453"
     CDS             complement(36638..37510)
                     /locus_tag="PC429_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF3845977.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(37801..39075)
                     /locus_tag="PC429_000454"
     CDS             complement(37801..39075)
                     /locus_tag="PC429_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF3845978.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39359..40516)
                     /gene="ftsZ"
                     /locus_tag="PC429_000455"
     CDS             complement(39359..40516)
                     /gene="ftsZ"
                     /locus_tag="PC429_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF3845979.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40706..41059)
                     /locus_tag="PC429_000456"
     CDS             complement(40706..41059)
                     /locus_tag="PC429_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF3845980.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41158..41832)
                     /locus_tag="PC429_000457"
     CDS             complement(41158..41832)
                     /locus_tag="PC429_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF3845981.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(41837..42535)
                     /locus_tag="PC429_000458"
     CDS             complement(41837..42535)
                     /locus_tag="PC429_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF3845982.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42558..43298)
                     /locus_tag="PC429_000459"
     CDS             complement(42558..43298)
                     /locus_tag="PC429_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF3845983.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEILTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(43568..44794)
                     /gene="murG"
                     /locus_tag="PC429_000460"
     CDS             complement(43568..44794)
                     /gene="murG"
                     /locus_tag="PC429_000460"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF3845984.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(44812..45942)
                     /gene="spoVE"
                     /locus_tag="PC429_000461"
     CDS             complement(44812..45942)
                     /gene="spoVE"
                     /locus_tag="PC429_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF3845985.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(45994..47349)
                     /locus_tag="PC429_000462"
     CDS             complement(45994..47349)
                     /locus_tag="PC429_000462"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF3845986.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASVIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(47368..48336)
                     /locus_tag="PC429_000463"
     CDS             complement(47368..48336)
                     /locus_tag="PC429_000463"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF3845987.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(48377..49750)
                     /locus_tag="PC429_000464"
     CDS             complement(48377..49750)
                     /locus_tag="PC429_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF3845988.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHAYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(49917..51896)
                     /locus_tag="PC429_000465"
     CDS             complement(49917..51896)
                     /locus_tag="PC429_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF3845989.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(51908..52255)
                     /locus_tag="PC429_000466"
     CDS             complement(51908..52255)
                     /locus_tag="PC429_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845990.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(52736..53671)
                     /gene="rsmH"
                     /locus_tag="PC429_000467"
     CDS             complement(52736..53671)
                     /gene="rsmH"
                     /locus_tag="PC429_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF3845991.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(53701..54447)
                     /locus_tag="PC429_000468"
     CDS             complement(53701..54447)
                     /locus_tag="PC429_000468"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF3845992.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(54841..56127)
                     /locus_tag="PC429_000469"
     CDS             complement(54841..56127)
                     /locus_tag="PC429_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF3845993.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDIIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(56142..57407)
                     /locus_tag="PC429_000470"
     CDS             complement(56142..57407)
                     /locus_tag="PC429_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF3845994.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSAKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRKLYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            57723..58820
                     /gene="ychF"
                     /locus_tag="PC429_000471"
     CDS             57723..58820
                     /gene="ychF"
                     /locus_tag="PC429_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF3845995.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        18442 a        10660 c         6335 g        23423 t
ORIGIN      
        1 acttaattta ttacttttat agaattttca caataaaaag tcattttact tccgtattta
       61 atgcaaaagt agaaattaaa cttaatataa aaaatatata acaaattttt tttatatttc
      121 tagaagaaaa gtatactctt ttttgtttaa taatgctaaa attgatttgt attgaatttt
      181 gattaggagg aaattattat gaaagctcaa agattaaatg ctgtacttga acaaatgaaa
      241 aaagatgata tttctcaaat gttagtaagt gaccctactt ctatatttta tcttacagga
      301 gtactaatcc atccaggcga aagactatta gcattatact taaatttaaa tggcaataat
      361 aaattattta taaatgaatt attcccagtt tcagaagatt taggtgttga aatggtttgg
      421 tttaatgata ctcaaaatcc agtagaaata ataactgaac atatagacaa aaatgctact
      481 atgggtgttg ataaaaactg gccagctaga ttcttattaa acttaataga attaggtgga
      541 ggaagtaagt ttgtaaattc atcttatata atagatactt taagaatgtg taaagatgaa
      601 gaagaaaaag agttaatgag aatagcttca aaattaaatg acaaggctat ggaacaactt
      661 aaagctactg tttctggtga acttacagaa aaacaacttg ttggtaaatt atctaaaata
      721 tatgaagacc taggtactga tggattttca tttgacccta ttataggttt tggacctaat
      781 ggagctaatc cacatggtga acctggaaat gcacttgtga aaccaggaga tgcaataatc
      841 ttagatatag gttgtataaa agataactat tgtgctgata tgacaagaac tgtattctat
      901 aaagaaatac ctgaaaaagg tagagaaata tttgaaatag ttcttgaagc taataagaga
      961 gctgaagcta ttgtaaaacc aggtgttaga ttctgtgata tagatgctgc tgctcgtgac
     1021 tacataactg aaaagggata tggacagtac ttcactcata gaactggtca ctctatagga
     1081 cttgaagttc atgataaagg agatgtatct tctataaata cagatactgt acaaccagga
     1141 atgatattct ctatagagcc aggtatatat ttaccaggtg agttcggagt tagaatagaa
     1201 gatttagtac ttgttactga agatggttgt gagatattaa acaaacatga caaagaaata
     1261 tgtgtagttg gttaattcta aaatcaattt gataaaatta aaaagccata taaaaatgtt
     1321 ttaaacattt ttatatggct ttaatttctc aatctatgat ttatttcaat ccattattta
     1381 tattgcagtt attaagattg caccctttga agtatatgtc cccatcactg aaccaatttc
     1441 acagattaca acatctttgt attttacatc cttttccata acttccctta ccttttgagc
     1501 cttttctggg caattagcat gtcctataaa taaaaccttt tcttctttat ctttaatttt
     1561 tgactctact aaagtaatca gctttttcaa actgtttcct tcacctcttg ccttatctat
     1621 aggagttaca acgccatctg caacttgtat tatcgcctta aaatttaagg tatttataat
     1681 ttttcctgct attggattta ttctaccacc cttaatagca ttttctaaag tttcaagtgt
     1741 tccaaaaaat ggaggattat ctttatattt atttattatt gaaacaatct cttcaatttc
     1801 ttttccttct tttattaata ttgctgcttt taacactaat tgtcctattc caattgaccc
     1861 actaagtgaa tctataacct caactttctt gttatttcct tccttttcaa acatatcttt
     1921 tgctaaaagt gcactattat aagtcccaga taatcctgaa cttatattaa tcactaatac
     1981 actttcttcc tgacaatggt atgcttctaa aaatttatca ggagaaggac atgatgtttt
     2041 tggtagtact ttactctcac tcatttttct atagaaagtt tcattatcta tttcctttcc
     2101 agatatataa ctttcttctc caaaagatac atttaaacct acaacttcta tgttgtattt
     2161 ttctattata tcatcaggta agtcacatgc actgtccact atcaatttta ttttatccat
     2221 attaacctca ctataagata ttattttatt tattcagtcg gtcgtacgac cgatttaatt
     2281 attattttat atattgaatg aaaaaaagtc aatagatata gattctcgct ttattgacaa
     2341 taaatatcca taagcttata atgttattaa tttgttataa atatacatgg aaaggtaggt
     2401 tttatgaaaa caaataatat gaaacaggct attttatcat ctgcaaccaa tttaatagcc
     2461 aaaaatggag ttcaaaatac tagccttgct gatattgcaa aagatgtaaa tataagtaaa
     2521 ggtactttgt attatcacta cgcatctaaa gatgatatta tatatgatat tgcggatact
     2581 catttagaag ttataactag tgctatacta aactgtgtaa aaaatgtcaa atcaaaaaat
     2641 tctcaaatag aaatggttaa cttaatctta gagaaaatat ctactataga aagtcgtggt
     2701 agggtacata tgtatcttat ttgtgaggca attacttcaa ataatgattt aaaggaaaga
     2761 ataagattaa aatatattga atggagaact actcttaaat ctgaaattgt tgaatcacta
     2821 gaaaaatata atagtagcga aaatgaacag gatgctgaat cattttcttt tttactcatg
     2881 tcaatagttg atggtttagt tgtccaaagt ttattaaaaa cagaaaaaat accttatgaa
     2941 aatatagctt cttttttagt taatcattgg atttaaactt aatacttaga taattaataa
     3001 aagtgaacta tttaagttat ctactttaac agttcacttt cttatttatt ctcaataatt
     3061 tttacttaaa acattcaatt tttatttaaa ccttcatgga atcacaaaat aaaaatctct
     3121 tataaattta aaatgttttt atttatgttc atgtgtatgc ccatctgaat gttcatgacc
     3181 atgtgtatgt gtatgctcat ggctatgctc atgagtacct gaaaaaccat catcatgatt
     3241 atggtcgtga cctacaccat ttacatgctc atgttcatgc ccatgactgt gagaatgttg
     3301 atgtgaatgt tcatggttat ctcctccaca gcatccttct cctccagtcc cacaacaccc
     3361 acttgtatgt ggattatgat gatttcttac tacaaatctc tctcttaaca taattcttat
     3421 acctcctagt atattattga gtacttcatg taccccatta actacagtat acatctaact
     3481 aggtataatc tcaatacata tggcattttt taacatatat aatcttaaag aaatttatcc
     3541 ttaaaacctc caccttttac ttcttcaacg ttattgacag aaataaaagc ttcactatcc
     3601 atatcataaa ttaaatcttt aaatttacct atttcatttg cagatattat acaataaatc
     3661 atctttttct tattttgtgt atatgcacct tctgcctcca aaaaagtgac tcctctaccc
     3721 attttattca ttatcacttt ggatatttca tcatatttat ttgatattaa aagtatagat
     3781 ttttttctat caaagcaatc ttttcctata tccatagtaa aagaaattat atacattgta
     3841 attaaagtgt acatcaccaa tttagctcca aataaaaaag ctcctgcaca tactacaaaa
     3901 aaattaataa ataaaaatgt atttttcata ggtatatcat actttctttt cattatggct
     3961 gctattatat caagtcctcc agcagtagct cttgctttaa aagttaaccc catacctata
     4021 ccagttaatg caccaccata aatacactgt agcatagtat catctattgg gaaatattta
     4081 gcaatatctt gtgttagccc cagtgctaga gaaaatagta acatattgac aaaacttatt
     4141 agcaaaaatt tattatcaaa atactttcta gctataataa atattggtat gttcatcaag
     4201 aaacttgata ttccctgatt aataccaaat aatttgtata atataacaca aattccagct
     4261 actcctccac ttaataactt ggctggtgct agatataaat ttattcctaa ggttgataga
     4321 atcataccca atataatcag acaatattgt aaaaatatat tattatcaat acttagcttt
     4381 ttactttcac catacatcaa aacgcacccc tttgcataat aaataaacca ccctgcataa
     4441 cacacgataa atttacaaat aaatttatca gaagtagttt cctaaaaata ttattcatcc
     4501 tcacttaaac gtttattcat taatattcta tcacatgaca taaataattt atatgattta
     4561 tatagattta tttattgtaa attttggaat attaaaacat tttattgaat agataattat
     4621 attacataaa aaatccctat agaaatctat aaactaattt ctatagggat tttggttaac
     4681 taactattat ttttattttt ttataacttt tatacctgtc atatggtcat ttaagttttg
     4741 ttcctctaaa gatttatcat ctactctgtt tatttctaaa gctaaagttt cagacttgat
     4801 atattcatta aaatggtcaa cagccttagc aatttcatca tcaccacagt attctattac
     4861 tatattatct aatacttcaa agttattaga ttttcttaat tgctgtactt tagatataaa
     4921 ttctctagca taaccttcat taatcaactc ttcagttagc ttagtatcta gtataacaaa
     4981 aagattgttt tcaacagata cattaaatcc ttccttagca gttataccta ttaatactaa
     5041 atccttatta aattcaaaag cttcaccatc taaatcaact gttaaagtct cacctttttc
     5101 aagtttatta gcagtttcat gagcatttaa tttacttaat gctccaacga ataaattcat
     5161 cttagcacct aataatggcc ctgcttcctt aaagtttggt tttaaactaa agttcatata
     5221 ctcatctaaa tccttagcaa atattacctc tttaacatta agctcttctt ttattaaatc
     5281 aactacatca cttattgtat cttcaaactt accatctact aacacttttt gtaatggttg
     5341 acgaactttt attctttcta cttctcttga agctctacct aaagtaacta agtttttagc
     5401 taaatccatt ttttcttcta atttagtatc tactaactct tcattacatt taggatattc
     5461 agctaaatga acagatactt ctcctgttaa gtttctatac atttcttctg acatatatgg
     5521 tgcaaatgga gctatagctc tacaaagacc atgtaacaat tcataagttg tattgtacac
     5581 tgctttttta tcttctgtta attcagttgc ccagaatctt cttcttgaac gtcttatata
     5641 ccagttagat aaatcatcat ttataaaatc ttgtatcatt cttaaagttt tatttaactc
     5701 aaatatagct aaattttctt ctacatcttt cattaaatta ttgaacttag ataatatcca
     5761 tctatcaagt tctggtctat ctttatattc tacaaaaaat tcagttggat ttatatcatc
     5821 tgtgtttgca tataaagtaa agaagttata tacattcttc atagtaccta taaatttact
     5881 ttggatttct tttaatccat ccatatcaaa tttagttgga gtccatggtg gagaaacata
     5941 tagtaaatac catcttaatg catctgctcc atattggtca aataattcca ttggatttac
     6001 tgtatttcct cttgatttac tcattttctt accatcttta tcaagtacaa ggtctggaac
     6061 taaaacactc ttatatggtg cttttcctaa tacaaatgtt gatactgcaa gtaatgagta
     6121 gaaccaacct ctagtctggt ctataccttc tgatatatag tcagctggga acaattggtc
     6181 aaagttttcc ttattttcaa atggatagtg gtgttgagca aatggcatag ccccactatc
     6241 aaaccaacag tctataacct ctgttactct tgtcattggt tttccacatt tttcacattt
     6301 taagtgtata tcatctacat atggtctatg aagttcaact gtttctggat ttacatcttc
     6361 tatagctttt tcagctaatt cagctcttga accaactgaa tctgtatgac cacattcaca
     6421 tctccatata ggaagtggag ttccccaata tctagttctt gatattgccc agtcatttag
     6481 attttctaac cagtttccaa atctcttctc tcctacaaaa cttgggaacc attttacacc
     6541 attattattt tctattaatt tatctttaag tttagtcatc tctatatacc agcttggttt
     6601 tgcatagtat actaatggag tttgacatct ccagcagtgt ggataattat gaactacttt
     6661 ttctttgcta tataatttat tttcagaagc tagccatttt attatttcaa catctagccc
     6721 atcttccatt acaaatctac cttcccaagg agtagatgta aatttaccag tttcatctac
     6781 tggttgaata actggtaaat catatctttt acctgtgttg tagtcatctt caccaaatgc
     6841 tggtgctgta tgaactatac cagttccatc ttcagtagtt acataatctc ctactgtaat
     6901 aaaaaatgct tttttgtcaa cttctacaaa aggcattaat tgttcatatt ctaaaccttc
     6961 taggtcttta cctttcattt cttctaaaac ttcataatct tcacctagta ctttatcagc
     7021 taaaggttta gatacataat atacctcatc acctttttta acttttaaat aatctacatc
     7081 agcatttact gttaatgaaa catttgatgg taaagtccat ggagttgttg tccatgctaa
     7141 gaagtattca tcagcatctt ttttcttaaa tttagctata actgtattag tttttacttc
     7201 tttatatcct tgtgcaacct catgactagc aagaccagtt ccacatcttg ggcaataagg
     7261 aagtatttta tgcccttcat atatatatcc ttctttgttg aatttgttta gtatccacca
     7321 tacagactct atatagttat tatctaaagt tatatatgga ttatctaaat caacttcata
     7381 agccattctc tcagtcatta ttctccattg tttttcaaat gagaaaactg actctctaca
     7441 tttttggtta aattcagcta ttccatattc ttctatttgt tgcttactag taagtcctaa
     7501 ctctttctct acttgtattt caactggtaa tccatgtgta tcccaaccag cttttctctt
     7561 tacttgataa ccagacattg ttttatatct acaaactgaa tctttaagcg ttctagataa
     7621 aacatggtgt actccaggat ttccattagc tgttggaggt ccttcataga atacgaaact
     7681 tgggtcatct ttcccttttt ctaaagtttt ttcaagtata tttatgtctt tccaataatc
     7741 aaaaacttgt gcttctgctt gttttacaga actgtctacc aatggcttaa atttcgccat
     7801 gtaaatcacc ctttcctact aaatattata attccaaaat tttttattct ttcacataaa
     7861 tttaaatact aaaaaactcg ccccctttaa accaaaggga cgagttataa atcgcgttac
     7921 caccctaatt ctatatgtaa actaaatact agctacatat aacactctaa tagtataacg
     7981 gtactaaccg gcacagctta gtagaattta atcgttcagc ctgcagctca agagtgattt
     8041 ttatctttat aaattcattg gttctcagca tacccaattc tctgtaaaat tatctaaaga
     8101 taaatgtctc tatcaacgct tttaaaaatt tatctgtgtt tatatgaaat tctataacaa
     8161 ataatttgaa tagtcaatat atttttgaaa aatataataa tttctatttt tcttacattt
     8221 ttatcctatt tttaatcttt gttatagatt tctcaaactc acacaattat tctacataaa
     8281 aaataaacaa attacaagct tattctaaag ttgtagcagc ttcatcatta ctataattat
     8341 acactgttgt tccttcaaat gcgtttacac atttttcatc tacatcataa aaaatctcat
     8401 caacacttct tatttcatct tctaacagtg atttaaattt atttctaaat atcttaaatt
     8461 ctttgactaa agcatcatac tcttttctta tctctataac acggttatta gcttgttcta
     8521 taatttgtct agcttttaaa tcagcttctt caacaattat cttagccttt ttattagccg
     8581 cactacaagt gtcttctgct gcactttgag cagttattaa agtggctttt agtgtttctt
     8641 caatattttc atacttgttt acttggtctt gatataaccc taatttttct ttaagagctg
     8701 tattttccct acatagagat tcataatctt ccttgatgat atctaaaaat tcatcaacct
     8761 cttcatctct ataccctctt aaaccttttt taaactcttt attttctatc tcaattggag
     8821 ttagcattac atttcctccc agctataatt taaattagtc cttatacaat taatcttgct
     8881 tgaactttta tttttccttt tttagtaata tctcctattt gtgttacctt agctcttcct
     8941 ttgcctctaa tagaaatcaa atcatcattt tttactattt tagaagttga agttatcttt
     9001 tcataattga catggacttt ttctccattt atgtattttg cactatcttg tctagatata
     9061 ttgtaaatac cacttatcac acaatctaac ctatctgaag atactgtaaa tgatacttct
     9121 ttatattttg aagaactatt aacaatattt tctctggaaa tttcctttac aattacatta
     9181 tttcttgaaa ccttttctaa gtttacaatt ataaaatcgc aaatatcact acttacaact
     9241 acttgacaaa agctatcatg tataattata tctccaattt tttctctttt tatacctaga
     9301 cccagtattg accctaagta atctttatga gatatatttt taaatttgaa attcccttct
     9361 atttgtaaaa atctaagagt atcttctata tcttcatatt ccatgtaaaa tggataaata
     9421 aatacagtgc ttctttcagc tcgttcataa cctccatcca cactgtattt tatttcattt
     9481 gtagaattta atattgctac agcatttttc acttcatatg gattaagaaa ctccgtagac
     9541 ttaacatcat agttttttat acaactatta gccttgtcta tcactttaaa cattttattt
     9601 ttcaagtcta tatcctttat gtgatttgtt agttttaatt tatccatcct atcaccttag
     9661 aatataacca atactaattg tcttaggaac atcaataata ctattgcaat taaaggagaa
     9721 aaatctatag gtccatttgt atatttatac attatagacc ttattggccc ttctattggc
     9781 tctgtaagat tttccaaaat atcaaaaatt ttactttgtc ttccatttgg aaaccaagtc
     9841 atcaagcttt ttataactat catccatgct attatatcta aaagataata aagtgcaatt
     9901 cttattgttc ccatatatta accgcctttt tactatttat tttgccaagg gaagaatgct
     9961 ttactttcta attctttttt catatttgca tctatatcca cattattagg agctagtata
    10021 aatattgatt tagaaacctt ttgtatactt ccatctaaga cataaactgc cccattcata
    10081 aagttaaata tagatttacg aacttcagaa ttagtcaatc cttccaaatt cactataact
    10141 gcccttcttt gttttagatg gtctgctata gttgcagctt catcataaac cttaggttca
    10201 actattacaa ctttcatttg gctagttgta tgtaaattta caattttatt tgcttttgca
    10261 gtactaaagc cactgttcat ttcttcttct tgaactatat cgtccatgcc actttcatat
    10321 tcatcttcaa tgtaatcttc ttcttcatca actatccaat tcttaaattt agatattatt
    10381 ccatttgaca ttattaactt cctccttaaa atttattcaa atttgagtaa ttatttttta
    10441 ttgtagtttc tttctccaaa gattgatgtt ccaactctaa caatggtagc cccttcctct
    10501 atagcaattt cataatcgtg actcattccc attgacaaaa catccatccc tacattaggg
    10561 atgtttagtt cttttatttg ttttgataaa tctttaagac ctttaaatac acctcttatc
    10621 tcatcttcat cttctataaa aggtgccatt gtcattagac cctttatttt tatattttta
    10681 tatttttctg acacttcttt gacaaagtct acaacttctt ctctatcaag tccatgctta
    10741 gactcttctt tagatatatt cacttgtact aagcaatcta tttctctgtt tatttgttct
    10801 gcacgttttt gaatttcttc gcataatgca attctatcta aagagtgtat catacaaact
    10861 ttatcgatta tgtacttaac tttattggtt tgcaaagttc ctatcaggtg ccagtttaat
    10921 ttgtcaccta taatatcata ctttctagct aattcttggg gtttattttc accaacagaa
    10981 gtgattcctt ccctcattgc ctcattaact aagtctacat caacagtctt tgtgactgcc
    11041 aaaagggtaa tctctttgct gtctctatct actttttttg cagctttatc aattttgttt
    11101 cttatctcag acaaattatc tttaatagaa cccattatta tcacctactt tatccttata
    11161 tttgtgttta tactgttagg tcttaaaatt attttgtcat atagattaac acttgtaact
    11221 ccatttatgt catttttata ataatccaca aatacagaag attcattctc ataagttatt
    11281 ccttctaata tgacaaattt tacactttgt gattgttcat caattacata tacaccacgt
    11341 ttattgtctt ttgtttttat agaagtttta ggtattttta aacattccat ctgattatat
    11401 attatatcaa attcttcgac acttgtatcc tgatttgcaa caatttggtt acttattttt
    11461 aattttacta ttattttatt attattttca tatattttgt aaacttttgc atctaatttt
    11521 tgctcatgat attcaattat tacattttga tttttttcaa agttattaga atcttcttta
    11581 ctgatgtatg tagatagata attactctgg tcattggcat ctttttctat acttcctttt
    11641 acatgaatct ttgcatcaac tttttcattt tttaacacca acgtatttac atttttgcta
    11701 attatcatta ctaatatatt aaatactaaa tatataagaa taccaagtat cactaatctg
    11761 gtatatttta cctttttcaa agactctccc cctataaaat tactttcatt ataagtactt
    11821 cttctaaaaa atgtttattc cttttttata agctaatatt ttacgttcta ataattattt
    11881 tttccatatt aaatttattt tttatcaatc ttcctataca agactgcatt atttatatat
    11941 aattgtatta taaagtataa gtttaattta cattttatac gttaagctta ctttatatgt
    12001 ttttatagtt atttttattt ttagatacaa tataaaacca attaagattt aaaataactt
    12061 ttgttatgtt gacatttatt taggtaatca tttacgaatc aggataacgt tatcataaca
    12121 aaagtttatg tcctaaaaaa aatacaagga ggattttctt atgtcagaaa ttttattagg
    12181 aacaggttta cttttattgg ttctatgttg tttcacaatt tttagttaca aggccccata
    12241 tggtatgaag gctatgggag ctctagcaaa cgctgcatgt gctactttct tagtagaggc
    12301 attcaacctt tctttacttg gtgatgtttt aggtatggaa ttttttagaa gtgttggagc
    12361 tgctaatggt agtcttggtg gagttgctgc tgctatactt gtgcctttag ccttaggtgt
    12421 atctccagtt tatgcagttt taataggatt atcttgttta gatttttcta tattatctgg
    12481 attcgttgct ggatactttg tttctttctt aattaagttt atagagaaaa aagtgccacc
    12541 tggagtggat ttagtggttg taattcttct atgtgcacca ctatctcgtc ttatagcatc
    12601 aaatgtagac ccattagtta atagtacact actacaaatt ggacaagttt tgatagaagc
    12661 taccaacatt agccctataa taatgggaat tatactagga ggtctaataa cagtagttgg
    12721 aacgtctcca cttagttcaa tggctcttac atctataatt ggtcttactg gtcttcctat
    12781 ggcaatagga gcattaggtg cttttggttc agcttttatg aactcaacac tatttgcaaa
    12841 attgaaatta ggttctaaaa aagatattat atctgttgct attgaaccat taacccaatc
    12901 agacataatt tcagctaatc caatacctat atattctact aatttctttg gaggagcctt
    12961 ttctggtatc attgtagcct tgatgggtct tgtaaacatg acaccaggta cagctacacc
    13021 tgtggctggt tttgctataa tgtttgctta taatccagtt caacaagttc ttataacagc
    13081 tggtttatgt gctttattca atgtaattgc aggattttta ggttcaaaaa tattcagtac
    13141 ctttaaagtt cttactgctg gtgaaatacg tggtactgat gtagaagaag aaattgctta
    13201 aaaaacaata agatttataa atttatatct caagtagtag agttttatat tgtaggcaat
    13261 tttattaatt gcaaacgttt tattttttat ttatgtttat gattattatg tgtgttgaat
    13321 atttagataa atttttattt attattttta tttagatttt tgtttagttt tttatttggt
    13381 tgatatatta ggaataaaaa ttagtatagt ttattactag ttagatggca taacttttta
    13441 gatacataaa aaattttata ttaattagat attattaaaa taaaagcgat acatgaattt
    13501 ttaaatgtcc atgtatcgct ttatttttgc cataatctta ataactgatt tttaaagttt
    13561 attatcttaa caaagttcat tatcttatat aatatttaaa tgcataggct tctttaattt
    13621 aaccatctaa atatttaatt aaacattatt atatgtaata taactaattt ttaaatgaat
    13681 gtataggagc tggaatcctt cctcctcttt gagtaaataa ttcagatgaa tttgtattta
    13741 taggcattac tggtgctcgt cctaaaagtc ctccaaattc taccatatca cctacatcac
    13801 atcctggggc tggtataatt ctaacggctg tagtcttatt attaatcata cctattgctg
    13861 cctcatcagc tatcatggct ccaagcgtac ctgctgtagt atttcctggc acagctatca
    13921 tatcaagacc tacagaacaa atagcagtca ttgcttcaag cttatctata cttaaagcac
    13981 ctttaataac agcatcaatc atacctgcat cttcagatac tggtataaac gctccactaa
    14041 gtcctccaac atgtgaacat gccattactc cacctttttt aacagcatca tttaaaagtg
    14101 ctaatgctgc tgtagtccca tgagtaccaa caacttcaag acccatttcc tctaatatat
    14161 ttgctaccga atcaccaata gcaggtgtag gagctagtga taagtctatt atcccaaatg
    14221 gaacatccaa tctactagaa gcttcctttg ccacaagttg tccaactctt gtaattttaa
    14281 atgcagtctt tttaactgtt tctgaaacaa catcaaaagg ctctcccttt actttctcta
    14341 aagctctctt aacaacacca gggccactta caccaacatt tataattcta tcagcctcac
    14401 caacaccatg gaaagctcct gccataaatg gattatcttc tactgcatta caaaatacta
    14461 ctaattttgc acaaccaaaa ccttttgcat cttttgttaa ttctgcagtt tcttttatga
    14521 tttctcccat ttctttcact gcatccatat ttataccaca tcttgtagaa cctacattta
    14581 ctgaagcaca tactttatta gtacttgcta gagcttttgg aattgactta attagtatct
    14641 tatcaccttt tgtatatccc ttttgaacta atgctgagaa tccaccaata aaatctatac
    14701 ctaaatcctc tgcagcctta tccaatgtct gtgcaaactt tacatagtca ttttcatctg
    14761 ttgcacctgc aataatggaa attggagtta ctgataccct tttatttaca attgggatac
    14821 caaattcacg ctctatatct tttccaacct tcaccaaatc tttagcagaa ctcataatct
    14881 tattgtatat tttttctcta gctttatctc catcagggtc tatacaatct aataaagaaa
    14941 tccccatagt tatagttctt atatccaact gttcttcatc tatcatcttt attgtttcaa
    15001 gtatatttcc tacattcact attatcacct ccccttactg tctactacag attatgcatt
    15061 gaattaaata tttcttctcg ctgaattttt acatccactg ataatttctt acctctttca
    15121 accaagtcct caaccgtttt ctcaaaagaa tttaatgatt tctccaaatc aactatcata
    15181 atcatagtaa aataatcctg taatattttt tgtgatatat ctactatgtt gatattttgc
    15241 ttgtataatt catcactaat tcctgccact atacctggtt tatcctttcc aattactgtt
    15301 aaaattgctt tcataataat atcctccctt atttttttat aaatcttata tttttacaaa
    15361 ttgaactatg tgttaacata tatttcattt atttgttagt aaaccacttt ttatgtatat
    15421 atttaatata ttttaatacc tatacattca tttgtcaatt ttttttcaat attctattat
    15481 ttttatacta tttagaaaat tcatatatta tatatgtttt ctcaacaata aatacagttt
    15541 tcataataat gaaaggtatt acttagcaat ctcatgattt tctaaataat acctttctaa
    15601 aaattatttt tttaatttta tgaatttaat tatacttttt ctagtatcag gatttacaat
    15661 taatatagaa ccaatcataa ttggtgttag tatacaaaat aaaaaaataa tatctttttt
    15721 tagattatat tctttagatt ctaatttata attattattt attattccat cataatcact
    15781 tatatttgac ttatttttat ttgtatcttg atttttgtta gaaatacttt ccctataagg
    15841 aaaagaatgt tcattggtac caaatactga ttttgagctt aaatcataat tatcatcatt
    15901 ttctgtaatt gaatttttat tgtaaaatcc tttttcttta tcagaattat tttctttatt
    15961 accgtcttta ggtatatcat gaggattttt attttcattg ctatcagtac ttgattctac
    16021 atcatcttta ggagtatttg gttgtttctc atcagaatta tcattttcat tattattatt
    16081 ttcattatta ttattttcat tattattgtt ttgattgtta ttactatcat tattttcatt
    16141 tccattattc ccatcttgat tgttatcatc ctcatctcta tccgggtcgt ctctacctcc
    16201 tcctccacgg tcaccatcaa tatcatcatt ttcccgttca tcatctggtg tctcttggtt
    16261 gtctccgcct cccattataa atcctggttt aaaaactata tttgaatacc cttcttttaa
    16321 tcctcctacc acttttactc taaaacaacg caacttaaaa tcttcaaaaa aaagtatcgc
    16381 ttgatctcta atatcctcct gttcttcaga tatccaattg attccatcgc ttgaatattc
    16441 aacaaaaacc ttacttgcac tatacggcat atcataaaat aaaacagcta catatccatt
    16501 ttttgtattt tgaaattcca gttccaaatt gtctattggt tttttctcta taacagatac
    16561 attcaaaata ggaactacat catcatttat tataattttt tctttattta acatttctgt
    16621 tttaaacttt cccgttatat taaaatcttc tcccttttcc aacttttcat aataatcagt
    16681 aaaatcccat tctacatcta tatcaagtcg attaccacta gatgtttcta ataccatata
    16741 tttgggaaat tttatatttt ctattttatc atctttactt acttttattt catcactaaa
    16801 tacaccaatt cccctatgac aacatttcac aatattatag ccagaatctt cagctataga
    16861 gggtataagc tcattataat catcaattat ataaactttc ttactttctg atataattga
    16921 ttgtgccaac ttattattat ctcttagtaa cccactttca ttgttgctac tatatgcttt
    16981 tattgaagtt tcttctatct caacatcacc tttagaataa acacctatag catgttctcc
    17041 attcacttca atatccttat ttagtattct aatatcattt tcagtataaa ttcctattgc
    17101 atcttttcca tttgccttta ttttttcaac agattcgtag gatgatttaa tatgtaagtc
    17161 tcctccttct tcaacgtata aggctgtccc atcagtagca attatgttgt ttataccaat
    17221 acttatactt ccaccatttt ctatatgtat tactccttct tttcctcctt cacctattat
    17281 attcaattca ttatcatcaa taataaatct cccatgattt tttatcaata ttttatattt
    17341 gttggtatct attgtaaaat tactgtcata aggaatataa aatctaaact cttcatctat
    17401 taccatatca caagaaagag tataaactat ttcttcttta ttatttttat tttcttgaac
    17461 ccacctttca aaatcttcaa gacaattttc ctcttgtcca aaaactttat gtggaaaact
    17521 tataaaacac aaaactatta tactcataaa aataaatatc tcatactttt tcatcttaca
    17581 ctcctagaaa tttttagctt tgaaacaatc ctgtcgaaac atatacttca cccactataa
    17641 tttttccaca attatcaatt attgtcaatt tatataagta tctttattaa ttgttataaa
    17701 taaaaagcta cttactaaag caaacagacg tttcacttta ataagtagct atatttttat
    17761 aacaaaatag ttataatatt agaacttcct tcatttacaa ttttttgtaa agtcttttgc
    17821 attttaactc ttatctcttc tggcatagta tataatttac tttgtaattg ctcttttact
    17881 aagtcatgta gtgatttacc aaacatattt gattcccata aatctgctgg ttgctcttca
    17941 aatacttcca taagatattt tatcatctct tcaccttgat tttgatttcc aactattgga
    18001 gaaacctctg tagatatatc tgcttttata atatgaagtg atggtgcatt ggcttttaac
    18061 ttgataccat attgttttcc ttgtttcatt atttctggtt cttccagact aagttcttct
    18121 aaagaaggag ctactactcc atatcctttt atcttggcat ctattaaagc actttctatt
    18181 ttatcatact catttttaac tttagatagt ctagttatca gactaagcag ttgataatct
    18241 ccttcaattt taaatccact tttttcttca aggacattat agaataaatc ttgctttgtc
    18301 tgtaaatcaa tatttataac accttctcct agctctacat tatcaacacc tgtatcttcc
    18361 aaaaactcta actcagaaaa tccttgtata ataccttcta tatcccttat tttacctata
    18421 tctataatac tttgttttaa tgttgttatt atgctacttt ttatccaatg atttctttca
    18481 agaccttcaa cccattttgg taagttgatt cttatttctg taagtggaaa atcatataat
    18541 acagcttcca taacctcctc tatatcttct tcttccattt caaccacatt cattggaaga
    18601 actggaactt catacttttc ttccaattca cttctaagca tactcgtttc ctcacttttt
    18661 ggacttaacg tatttaaaac cactgcaaat ggttttttaa gatttttcaa ttcttgtata
    18721 actctctctt ctggttctac atagcttttt ctgtcaattc cagtaactga accatctgtc
    18781 aagacaacta tccctattgt agaatggtct tttataactt ttttagttcc tatctctgct
    18841 gccttttcaa atgtcatagc ttcttgtgac caaggagtgg atactaatct ttgttttcca
    18901 ccttcctcat gccctaaagc tccttcaact atgtatccaa cacaatctac cattcttact
    18961 tttaaagata ctgtgtcttt tatttttatt tctacaccat ctgctggtac aaattttggt
    19021 tctactgtca ttatcgtttt acctgaacca ctttggggta tctcatctct tgttctatcc
    19081 tttttaaatt cattgtctat attaggtata accaactttt ccataaattt tcttataaaa
    19141 gttgattttc ctgttcttac aggtccaact actccaatat atatatcccc ctgagtcctt
    19201 ttggatatat cttcgtatat gttattattc atattattcc tcctatacat ctaatcttat
    19261 taaattatat ttagagttaa gggttaatat tcttaaaata gaaaaataaa gaccagcaat
    19321 attaatttaa tttacatata gaaatttgta aatttcaatg taaattaaat taatattgct
    19381 ggtccaaata gtagtgtatt atattaactg actttagtag cattttcatt tgaattatta
    19441 ttcatctcaa taagttcttt acgtctttct tttgtcatgt tcatccatat aaatgtaaac
    19501 accaatccaa ttatggctac tatagtaaga actatgaagt agtacctata tccagttaca
    19561 cccggaaatg catccaatat cttccctgct gctaaaggac aaagtacttc tggaagatat
    19621 cctaacgtag caactattcc tatagcagtt cctgatattg ataatgaata attaccttct
    19681 tcaagtaacg aatagtgtaa accttgaatt gcatacatag atacatatat tgcaacacat
    19741 ccaatcaata gtatataaat catagatgat tttgttggtg taaatattac acctaaaaga
    19801 cctgcaatca taagtataaa tccataagat ataatctttg atgaaccaac tttatctcca
    19861 agtaatccag aagcaactga tgctactggt ctacaatact gagctaatat tgagattgca
    19921 gatgcaaaca ccacagtagc tccaaatgct tcagttgaat aaggcgtaaa ataataaaat
    19981 gacatattag ctgaatatga acaaaacatt attattgtta ttatccatgt atgaggcatt
    20041 tttattactg caaaaaaggc ttctttatca aattttgctg atacctcttt ttcattatcc
    20101 tttaatttga aaaatactaa tactccaact attacattta ttaccgagta taatactata
    20161 atagctgtaa gaccagattt atcacttact tttgatgaaa tatacccaaa tagtatcaat
    20221 actagagata gatgaattgc attggtaatt ccacgaccac tttccataaa tccaaaagct
    20281 tttccttgtt cattttcaga tgctaacatt ctaacagctt ttacaagagc tggccaaaaa
    20341 gtaagtattg ttgttattcc ccataaggca tggattaaaa acacaacaat atatggagga
    20401 taaagtgtta atacaagacc agatattcct gtaataatta aagaacctga aagcaatttt
    20461 ctggctgaaa tacggtctgc taaaaaacca ccaaaaaggt atgataccat tgcaagtaga
    20521 ccataaatgc ttcctaagct tcccatttgt gtatttgtaa gattaaatgc ttgtgcaaaa
    20581 gtatcataat aataattctt taaatatggt aaagcatata taaatgctcc tgctacaaag
    20641 agtattatca agactttgaa atttttccaa atgcttggtt tatttacttc aactgacata
    20701 cttcctcctt atgtaaatac ctgtaaattt tcatgataat taattattgc ttattgtatt
    20761 gtttattcaa tatttcggaa tatttattaa tatattaggt taatctatca ataatttatg
    20821 gtgagttatg agctaaaagt aaactctttt agctcaccat aataattata tttttattta
    20881 tactaacgtc cagagttatt agatatcttt ttttcatagt tcttccatag cattaaacct
    20941 gttattataa caaaaagacc tcctcctatc atttcaatct catagattat attatctttt
    21001 aaagtctcca taactggtgc cgttgagaaa agtatgccaa ctacagttac tacaagtacc
    21061 attctggcaa tattgataca aactgaatca ctttttgcaa ttgaatatgg tctttcttca
    21121 tttggtcttg tctttctaag tctaatataa gatgtaaaaa gcaagacata tggaaataat
    21181 gctgttaatg ctgtcatagt aacaagaaca ttatatatat tatctactga tggtaataag
    21241 tttgttccta caagaagtag actcactatt atagcctgaa ctattactgc atttgatggt
    21301 atattgtatt tatttgtaac agtcaattgc ttaggaaata caccttctct tacatttcca
    21361 aaaagcatct ttactggacc tgctatataa agtattaatg ctcctagtac tgataaagtt
    21421 attccaaatg ctactattct tataaaccaa cttcctatac ctaaatcttg tgcaacttta
    21481 gctaatgcat ctagtatccc tgtagatgct gcaattttat ctggaggaag tatcattgtt
    21541 attgctactg aacctaagat atagattcct cctattaaaa cagccacagt taatatagct
    21601 ttaggaaagt ttttcttagc attatccatt tcggttatga aattagcagt cgtttcagct
    21661 cctgataatg caaatattat agcagatata gaaactaatg aattggcatc tatttttggt
    21721 attatatttt ggattgtata tatcgatgct gatggagctt ttttgagaat aactacagac
    21781 ataaatgcca tcactataag taatatagca ggaattgtag aacctaatgc tcctgtattc
    21841 gtaaaaaatt ttccaaatgc catcccacga gtacttacta aggacaacag ccaaaatatt
    21901 atgagtgaaa gtgctaaaat aaacatctta ttgtttgata aatctggatt tccaagcata
    21961 tatgcaatat ttatagctaa aaatgttaag aaagaagaat accaaaatat ctttgatgtc
    22021 caatttagcc atgacaccat aaatccccac ttctctccat aagcttgttt aacccattct
    22081 ccaagtccac catcttttgg atatgttgca gccaactctg cacatataag tgatgcagga
    22141 acaaagaata taaaagcaaa caataaccaa actggtatag aaccaagacc caatccaaaa
    22201 ctagaggcag tagatttagc tatccatcta atcccaaata gtgctgatat attcataaat
    22261 actaaatccc aaaatccaat tttctttttc actgtaattc ctccaatttc aaatttattc
    22321 aaactcatac actatgtatt gatttaaata tcaagtataa gttcttcaac acttcttgga
    22381 taattagtta taattgtagc accattctta cctactattg ctgtatctga gtgtctaaat
    22441 cctccaactc caggtatata tatccctggt tccatagaaa ataccattcc ttcttctaat
    22501 attaattcat tgtcaaatct taaatatggc tcttcatgtt ctgataaacc taaaccatga
    22561 ccaattctat gatttgaata taactctaga ccatactttg caacaacagc tcttgctgct
    22621 tcatccacca ttcttgctgg tataccagct tttattgtat ctaatccagc ttgttgagcc
    22681 tctactgcaa tcttaaacac ttctttttgt ctttctgttg gtttgcctat aataaaagtt
    22741 ctttcatttt ctgctctata gttttcatac caaacctgtc tactatgtat tactatatct
    22801 cctctttgaa gtattctagt attagaatct aaatgaggtt gtgctgttct atcaatccct
    22861 gaacaagtcc agtttgcaaa tcctatatat gtatcaggat aattttcaga aacatatttt
    22921 agtaaagcat tatcccctgc aacgtcaaac tccaattcac tcatcccaac tctaacattt
    22981 tctaaagacc cttttattcc tatatcagat aggtaacctg ctatcttcaa aaattctatt
    23041 tcttttgcat cttttacata tctcatttca aatattttac ttccaacatc tttgatagca
    23101 aatgacttat cagttatata ctctgaaaag cttgcagata ctatatcttt ttctattcca
    23161 acagttgttc ctttagggta tttatttaaa attatatcta agtaatgttt atgtgatatt
    23221 ccatgttctt tcatttctgg tatttcataa tacacatata cattatcaac ttttgcaact
    23281 tctttagcat gtaattcttc taatccaggt actattaatt ctattttatc ttgagttact
    23341 actgttacaa ttggtctaga ataagttatt gctttaaatc cactaatata aaattgattg
    23401 tctggttcaa atattaaagc aatttcaatg cttttttcat tcatgtactt tctcaatctt
    23461 tctagtctca tattatttga cataatgcac ctctttctta atattcagat aatttattca
    23521 atacaacaag gatgaatata ttattttaaa attcttaata tttttagtac ttgtcgtatt
    23581 tgtatgtaaa ttaattttat ttgtgattat tttatcataa tcatgattat tttgtcaatc
    23641 atgattttat tttaaaaaat tttttatatt ctagtgattt taaattatct aaaatataac
    23701 gagtatacat tgtattttgt aatattattc ttgatttatg gtaagtttaa tgataaaatc
    23761 acgattatat aaactatatt gattatttgt aggtgaataa aatgacaaat aaagaattac
    23821 aaaagaaaag gatattgatg tatttcatag aagctgctca aaatattatg gaaaacgaag
    23881 gtatagaaaa tattacactt cgtaaggttg cagatatggc tggctataat agttcaactc
    23941 tatataaata ctttaaaaat ttagaccatt taatatcatt tgcatctata aaatatttta
    24001 aagattataa cttgaatatt tcaagatgta tagaaaatgt caatgatgaa tataagaaat
    24061 atataattat gtggaaatta ttttgtaagc attcttttga taatgctcaa gctttttacc
    24121 aggtattttt taacttgtct agtgatgaac ttagctatat aacaaaacaa tattatgata
    24181 tgtttccaga agatttaggt attcatgata gtgacatatc tttaatgatt acaggagaaa
    24241 gtataaagga aagaaataag atactattaa acaaccttgt agatactgga tatataccag
    24301 ctaaagattt agacataatc aatgatataa ttatttcttt ttatcaaaat ctattattct
    24361 taaaaaagaa cacaggaaaa aattctgatg atgatgaatt aactatgaaa atgatttctt
    24421 ctattgaata cataataaaa catgctgaat aaaattaaaa tatggctgtc tcaaaataga
    24481 gataagcctt ttttattgtt gaaataataa aaatatattt aatttcatat aaaaaagagt
    24541 gcctcgtaaa ctaaaaagtt cattttgaga cacccctaaa ataaatttat tatttattat
    24601 acaatagttt atatatcatc acctaaaact acttcttcca tttcatgctt tttacttctc
    24661 atcattagtt ctattccaac ttctttagga tttgaacctt tctttattac agaatatata
    24721 gcattagtta taggcatatc tatatctaat ttttcagcaa catcatgtgc tacttcagta
    24781 gcagttatgc cttctacaac catctttact tcttttaaag tatcttctaa actcatacct
    24841 ttacctatga gtataccagc tcttctattt ctactatgca tacttgtaca agtaactatt
    24901 aaatctccaa tacctgaaag acctgcaaag gtagacatat tagctcccat agcaattcca
    24961 agtctgctca tttcactaat acctcttgtc ataagggcag cttttgcatt atcaccatac
    25021 cctaatccgt cacatatccc agctccaaat gctataatat tttttaatgc tcctccaagt
    25081 tctactccaa ctatgtctgg attcgtataa actctaagtt tagggctcat aaataaatct
    25141 tgtatcatct gtgctatttt taaatcttta gaagcaacta ctacggtagt aggaatatcc
    25201 cttgctactt cttcagcatg agatggccca gatagtatta catatggatt ttgtggtaat
    25261 tcttcttcac agacttgaga taatctaagc ccagtccctt tttcaagacc tttagctaca
    25321 ttaacaataa cttgtccttc ttttataaat ggttttattt tttggcaagt acttcttaca
    25381 gcctgtgatg gaactgctaa aactatcacc ttacaatctt ttatagtttt ttctatatca
    25441 gtacttataa ttatattatt tggaaataat actcctggta aatagtctgt attttcttta
    25501 gttctgttta tttttttagc ttgttcctct tttcgagtcc acatgctcac atcattacct
    25561 ttttttgcaa gccctaaagc taatgcactt ccccagcttc cagtacctaa tacacatact
    25621 ttctccatgt ttaacaccac ctgagtataa tatatttact attctttctt ttgaccaatt
    25681 tttctctctg tcccatttaa taatcgcttt atatttgctc tgtgattata tattactgat
    25741 gcagttaaga acagagctac tattaaacca gctttatttt ttaccataat cataaatatt
    25801 ggtgaacatc ctattcctac aacagaaccc aatgaaacat actttgttat agctaccacc
    25861 aatataaata ctgctagaca cattagtgtt attactggat ttacagctag cattgcccct
    25921 agtgaagttg caacaccttt gcctcctcta aatcctaaaa ctgctggcca attatgacct
    25981 gctacaacac atataactgc cagatatcca gctaaaagag tatctattcc aaccaatctt
    26041 gctgcaaatt ctgatattaa aacagccatt acacctttaa gaacatcacc taaaaatgtc
    26101 attgctcctg ctctttttcc aagtgtccta agtacatttg tcgaacctgc attaccagaa
    26161 ccttgagtcc ttatatcaac cccagcaatt cgctttgcaa ctatgtatga ggtagatata
    26221 tttcctaaaa gatatgcaac tacagcaata attatataac taaatatctc cactgaattt
    26281 ccccccttta gttatatttt aagatattta ttttttattt ttttgtctat actcaaactt
    26341 tatggaagtt ccttcaaatc caaaattttc tcttatctta ttctcaaggt atctttgata
    26401 tgagaagtga gttaaatcct tatcatttat aaacaatgta attttaggag gtctaatgtc
    26461 agtttgagtc ccatagtaaa tttttaatct tctacctttg tcagatggtg gttgatttaa
    26521 cattacagct tctccaatta cttcattcag tgctgaagtt gatattctct tagaatgttc
    26581 atttgacaca tattcaactg tatctagtat cttgttcatt ctttgattag tttttgcaga
    26641 aacaaatacg attggagcat acatcataaa tggaaatttt tcttttatat cttttgtgta
    26701 attactcata gtcttattat ctttctctat taaatcccat ttattgatta caaatatgca
    26761 tccttttcct tcatcatgtg ctatacctgc aacctttgta tcttgttctg ttacaccttc
    26821 taaggcatct attactatca atactacatc tgctctatct acagcactca tagctcttat
    26881 tacactgtat ttttctattg tctcatatat tttacttttt cttctaagcc ctgctgtatc
    26941 tattagaaga aatttctgtc catttttttc aaaataagta tcaattgcgt ctctagtagt
    27001 tcctgcaatt ggacttacaa ttactctttc ttctccaagt atcttattga gtatagaact
    27061 ttttcctgca tttggtttac ctgttatagc tactcttatt atatcttctt catactctgt
    27121 atctaatcct gctggaaaat tttcaactat ctcatctaat aaatctccaa atcccatact
    27181 attggctcct gaaactgcaa aaggcgttcc aaatcccaac tcataaaaat cgtatatatt
    27241 atcaaattga ctttgactat caatcttatt aaccactaag attactggtt tttttgtctt
    27301 tctaagcaat tgtgcaattt ctttatcagc agctgttatt ccagctttac catcaactat
    27361 aaaaagtatt acatgtgaca tgtccattgc aagcatggct tgatttctca tttgagataa
    27421 tattatatct tcgctatcag gttcaattcc tcctgtatcc actaatgtaa aatatttatc
    27481 taaccattct acttcagcaa atattctatc tcttgtaact ccaggtgtat tttcaactat
    27541 tgatatcctt tttcctgcaa atttattaaa tattgtagac ttaccaacgt ttggtctccc
    27601 aactacagct acaactggcc tacttatact cattttattt ctccttccat cttttgtttt
    27661 ctattttaaa attttatcca caaaatcttt tccttcattc aaacaaggga taatttttat
    27721 tctcataagt tcttctaatt gatttaattc aatattatct aagaaaattt cttcatctgc
    27781 ttttagcata cttcttgtta tataaagggc ttcacctaat tctttatctt caagttgttc
    27841 ttttaaatcc ttagctgtta aaagacctga tacagttatt gtctctccaa aaaacttgtt
    27901 tttgatttca tatacattta cttgaagatt tttaaacttg tccatcatag catctgccat
    27961 actctgtata aattcatatg cagaatgacc agtagctatt gatactttct tctctctttt
    28021 tagaatattc tcaggcaata tatttagata ctcttcaatc tctgttttta acttacgtat
    28081 catacctaca ccatcttcaa actgtaaaaa accttcatat tcttcatatc caggcaattc
    28141 actattagat agtatataaa attcatctga taaaaatgca aatcgagtac ctaatttttc
    28201 aagatatttc tgttgtattt gatgtatttg ctctatagtt ttacttgcgc tcttatcatt
    28261 aaatatattt aattctacta gattttctct atgctttgta atcccaactg gaactatcgc
    28321 aacactgttt acatatggat aaagttgagc tagttctttt attgttctat ccaattcctt
    28381 tccatcattt atacctggac ataaaacaat ttgacagttc atttctatat gagcatctgc
    28441 caatctcttc attataccat ataattttcc tgcaaattta ttgcttatca ttttttgtct
    28501 taactctgga ttagttgtat gcacagatat atttatagga cttattctat attttataat
    28561 attatttata tcttcttcac tcatatttgt aagtgtgaca aaatttcctt gtaaaaaaga
    28621 taatctcgaa tcatcatctt taaaataaag agtttctctc ataccttctg gcaattggtc
    28681 tataaaacaa aacatacact tatttctaca gcttttagct tggtcaatta tcggatttgt
    28741 aaattcaaca cccaaatctt catcatagtc tttttctatc tcgtatatgt agacttcgcc
    28801 atcttttttt tgtatttcta catctaaata ttcatcactt agtaagaatc tatactctat
    28861 tatatcatgt attggctgtt cattcacaga tataagtaaa tctcctacct ctatacccat
    28921 ttcttctgca atactatctt tataaacctt acttataata ttatttactt tttttacatt
    28981 tacttccaat attttcacca ctcttttgta ttcatcaatg cttattataa tgtattttaa
    29041 tatgaaattt atttatttat tatattttat gatagcattt ttaagcattt catgtcataa
    29101 taacacattt taattctaat agtcaatata agtaaatagc tataaaatag attagaacaa
    29161 ataatattca aatttaagtt tcttaaaaat ttaaatatta tttgttctta aaaaaatttt
    29221 ataaatttaa tgttttacta tgattaacgt cccatttgtc atatcatgaa ccattccacc
    29281 tacacacttt gacatcaaca atgccttatt ttgtaagtct ttctcatcat ttgcataaaa
    29341 tattggacaa cctccaccat ttttcatatt cttatcaagt gtaactatag ctagggtata
    29401 ttcatttaaa cctatatcca ttcccatctt atgcacttcc tttctttcct attttataat
    29461 tttttagtga taagtttttt cctttagagc ttgaaagtat tgggcatgac tttacagctt
    29521 ccattacttt ttccacattc ttatctactg gtaaataagc aatcattata ctttcatcct
    29581 ctttatttct tctaggtaat ggtacgaatg aaggttctcc ataatctcca tataaaccta
    29641 accttgaata tatattgtat actattgctt gtctttgacc ggagtcaaat aatattcctg
    29701 cattagcata atccttattt ttaggaatta tttgtattcc tacacctcta ttaatatata
    29761 cttctctatc gctttttaga cctatatttg ttataccttt taaatctccc acctttagta
    29821 tagattcgtc cacaaaacta atttcagcag gcattacatc agctatgtct ccaatacttt
    29881 ttctagtaag aagttttttt aatataaagg ctaacactaa tccacaaatt gaacttatta
    29941 ccaaactcat cccttcactt attttcgttt ctgcaagtat tacataatac aatcctattg
    30001 ttaaaaaaga tgtaactata cacatataat ttcttacttc ataggttcta gctatctctt
    30061 ctatgaatgc agctcctctc tttacaagtt gaacttcctc taagttttgc aatgtatttc
    30121 ttctatttgc tctcacttgt ctaaattgtt ctgctgctag agataaaaat gttatggatg
    30181 tataagaacg atttataaga gctggaacca ataatgctcc taatgaagaa gcgacgaatg
    30241 ctaatataag ttgagatagt aaaacatttg gttgcgttgg atactgtttt tggtctaaat
    30301 tcaacattat aattctagat gttataccaa taataacagc aacaaaaaat actctatcca
    30361 ttaaatcact cctaaaatat ttaatttgaa attttacatg taaatctaca aagtctttct
    30421 agaaaaatca ctctgctttc cttttgatgt ttctatgata ggtgtacttt tcactacatc
    30481 tatcatgaca tctatatctt tgagtattgg catatatggt ataaccacag tacttttttc
    30541 taaattggtc ttagatatag caacaatatc aacctcatct acatctctat ctatccccat
    30601 atgaataaat agattatgta ttatagcttg tctttgccct atatcattta ctattccaaa
    30661 tgcacctaaa tctcttggta taacttctat agccagacat tcatttctat acttttctct
    30721 tgtactttct agacctatgt tagttattat tacaccattt accattaaaa taggtccttc
    30781 aaaacttatt tttgcaggaa ctatatctgc tatatctgct atactgttcc ttcttaaaaa
    30841 tcttctaaaa atcaaaccaa caatagctcc tgacacaata gctaagattg tgcaataaaa
    30901 aaagctaagg ccatactttc ttgcaaaaac gatataaact atcgaagata ccagtgcaga
    30961 aaaaagactt atataacttc tagattcata cgtagatgct atttcttcaa cgtaagcgtc
    31021 tccttttggt actaattctt cattatcgat attctttagt gttattcttt cttgttctgc
    31081 aagaccttga aactgttgta ttgctactgc aaaaaatgtc aaggctgcaa actccttatc
    31141 tataagtgca ggtaatgcta tagcacctaa cgaagctgaa agcccagata taattatctg
    31201 ttctaaataa tcttgtggtc ttgatgggta ttgtttgtct gtaactctta aaactaatcc
    31261 tctacataat attcctacaa ctaatgcaac tataaaagag tgtctaaata aatttgcatt
    31321 caataaactt tcttccattt tctcagctcc ttctcttata tagtttgtgc aaaaaacaaa
    31381 aaaaattccc ttctatttaa ggaaattttt ttgtaatatt gtaactataa attcgctacc
    31441 aatgccaagt tcacttttta cttttataca tccatttagt gatttgacta tatgctttgt
    31501 tatagccaat cctaaacccg ttcctcctac atccctactt cttgctttat ctaccctata
    31561 aaatcgttca aatatgcgtt ctacatcttc ttttggtatc cctacaccat tgtctataac
    31621 tttaattacg attttttctt catcttttaa tactacaact tgtacaatcc cattatcttg
    31681 ggtatacttt atagcattat caactaggtt taagaacact tgctttatat agtctttatt
    31741 ggatagtata cttattgatt tatcatcaca tttatataaa acttctatat ttttttcact
    31801 agctgaatgt tgagttattt catatacctc tttaaataca tcatatacta caacattttc
    31861 cataagaaga gtatctttat tttctacaaa tgataacaac aatatatcat caatcaacct
    31921 ttttaatcta tctgattcgc tttctattat ttctaagaat ctatttctag tattcacatc
    31981 tatattttca tttgacttta gtgtttcaac aaacccactt atagatgtaa gtggtgtttt
    32041 aagttcatga cttacatttg caacaaaatc acttctcata ttttcaagtt taactttttt
    32101 agtaatatct tctatattta ctatcgaacc tattattacg ttgttagtat tctgaagata
    32161 aactgggtct acttttatct tatatacgat atcatcttta gtagtaatat tagcagtttc
    32221 attatcttta gaaccaataa attgaagtat ttgttttaat atttcttctt cttttattac
    32281 aacattaata ttatgacctt caactaattg atttggttca ctctttatca tttcttttgc
    32341 ttcatcattt atcagaagta tatttccatt tacatctata gctaaaattc catgagatat
    32401 actctttaat atagacctta actgtaaatg tttatactgt acttcttcta aggttttatc
    32461 catattcttt atcatatgat taaaatttct agctaattcg ccaagttctc ctttggctga
    32521 tatgttaagc ctagcatgat attcattatt acttactttt tttgagacgt atataaactc
    32581 ttttaaatat ttccttaatg ttattgtgta tcttacagct aatataacaa tggctatgga
    32641 caacataaca ataaaaaaat atatactatt taaatcttcc acaagataac actcctaaaa
    32701 tataaccttt ttaatctatt ttgtatccta cacctcttat agtctctata tatttttccg
    32761 atttatcttc atcttctatt ttttttctta aatatcttat atgaacatct actgttctag
    32821 tttctccata gtattcatac ccccatatct tatccaaaag ataatttctt gaaagtactt
    32881 ttcctttatt ttgaattaat aattttaaca attcaaattc cttaagagtt aactctatct
    32941 tttcagagcc tttggtaact tcatgttttg ataaatctag ttttaaatta cctgtagtaa
    33001 gtatattgtt ttcttcacct aagctagtca aattatatct ccttaaaaca gaactaatcc
    33061 tagctaacaa ctcttttata ctaaatggct tagttatata gtcatcagca cctatttcaa
    33121 gcccttcaac cttgtcattt tccatatttt tagcagtaag cattataaca ggaatatttt
    33181 ttaagtcctt atcacttctt atttttctca aaacttctat tccacttata tttggtaaca
    33241 tccaatctaa aagtattaaa tctggtttaa tttccttagc ctttataaat ccgtcaaatc
    33301 catcatatga ataactaact tcgtaatttg acacctccaa attgaatttt aatagctcaa
    33361 ctatatgcat ttcatcgtct ataacaagca ctttagtatt cattaaaaag ctcctctcta
    33421 ttttatctgt agtaccgttc ctattcgttc tgatgcctta ttatgttttc tataagagtg
    33481 gaatttatct gctctacaac tagtgcataa atttaaattt attatatttt cttttttaac
    33541 tccactacat ctaagcatat actcattaat tttccataaa tctaaatagt aacttccctc
    33601 ttttattata tagaactttt catcattatt tgtaagaatt gtgttaaatt tttctactaa
    33661 atcttcagac acttcataac aacatggtcc aattgatggc ccaattatac atactaaatc
    33721 ttcaggaact gttttatagc attcagacat taattttatt gttttatgac ctatattact
    33781 aaaagtccct ctccaacctg catgagccag tcctatggct ttatttttag catctataat
    33841 tgatataggt acacagtccg ctgtaaacag taaaagtggt actttttcca agtttgttat
    33901 aagtgcatct ccatctcttc ttttccctat agtatcttta ttgactatat taactatatc
    33961 tgaatgtatt tgagaattac tagttagatt ttcaaatgtc aaatcacatt ctttacatac
    34021 ttttaacata tcttctctat tttttgcatc tatattctta catgttataa ctagatttac
    34081 agaatttaat ttttcatcaa gttcttcaca attaccactt aaattaacat actctttaat
    34141 atcttgtaca ttttttatat ttatataatc tttcatcatt tgtctccttt ttctatcagt
    34201 atacttttaa gttcatttac aaaagtattt atatctttaa attgtctata cacagaagca
    34261 aatcttacat aggcaacctc atctaactct ttcaatttat ccattaccat ctcaccgatt
    34321 tttttggttt caatttcttc tataaaacat ttatttattt cattttcaat atgtacaaca
    34381 atactttcaa tctcgtcaat agaaacaggt cttttttcac atgcttttag caatccatat
    34441 tttatctttt ctctatcaaa atattctcta ctattatcct ttttaatgac cattaaagga
    34501 gttgtttcta ttttctcata cgtagtaaac ctctttttac atgattcaca ctcacgtctt
    34561 cttctgatag attttaaatc tgtatgtcta gaatctatta cctttgattc tttataatta
    34621 caataaggac attgcatcta tattcctcct ttgtttaact atatctcaaa ctcatctaag
    34681 cttaattctg aacctatatt tactattatt gtatcacaac ctattttaag tatatcattc
    34741 caaaatataa cttcttcatc ccctcctctt gaaaaaaaac ttctacttcc atctccaaaa
    34801 atggtaaatg agactacctt accttcgtga atatccatgt ctatgtctat tataaacccc
    34861 atcctttttc cattttttac atttataatt tctttttcca ttatatcaga aactctaatc
    34921 atattgtact caccctttct ttaaaaataa atatgtatcg aagtaaaaaa aaatgaccta
    34981 tataggtcat ttaaattaaa ataatgttta cagtccatgg attacttata catattttct
    35041 catatttttt aaagcatttt tttcaattct tgatacttgt gcttgagata taccgatttc
    35101 atcagcaact tctatctgag ttcttccttt gtaaaacctt aaatctaata ccaatttttc
    35161 tctactattt aatttcttaa tagcttcttt aagtgatatt tcttgcaacc aattttcatc
    35221 agtatctttt ttatcttgaa cctgatccat aacgaatatt gcatctccat tgtcttgata
    35281 tactgggtca aacagagata ttgggtcttg tatggcatca agtgccatta caactgattc
    35341 tacttctaat tctaattcct ttgctatttc tgatactgta ggttctttag aatttgttct
    35401 aattaatctt tctctaactt gtagtgcctt atatgctata tcttttaatg acctacttac
    35461 tctaattggg ttattgtctc ttagatatct tctaatttct cctataatca taggaacagc
    35521 ataagttgaa aaccttacat tttggcttaa atcaaaatta tctatagctt ttataagacc
    35581 aatacaacct atttgaaata aatcgtctat attttctcct ctgttgttaa acttttttat
    35641 tacactcaag actaatctta aattccctct tacaaactgt tgtctggctt cttcatcacc
    35701 attttttatt tgtaataaaa gttccttcat ttgcttattt ttaagaactg gaagttctga
    35761 tgtattaaca ccacatattt caaccttatt aacttgcata tattttcagt cctctctcaa
    35821 aagtattcat ctattgaaat tatttacatt tccaattttt ttatactttt taagaagaat
    35881 taatttagca ttatacaaat tttttcattt ctttttgcaa ccttgagatt attttctttt
    35941 caagtctaga tatgtaggac tgtgatattc caagcattcc agcaacttct ttttgtgttt
    36001 tttcaatacc tttatcaata agaccaaatc ttaattccat aatttgcttt tctctgtcac
    36061 ttaatctgtc aagtgccata accaacaaat ctctatctat ttcttcttca attattttgt
    36121 atatttcatc attttctgta cccaatacat cagataataa aagttcattt ccatctaaat
    36181 ctatatttaa aggttcatca aatgatactt ctgttttctt tttattattt tttcttaagt
    36241 acattaatat ttcattttct atacatctcg aagcataagt tgccaatttt atatttttat
    36301 tcagtttaaa agtattaact gcttttatca aacctatagt tcctattgaa atcaaatctt
    36361 ctacatctat acctgtattt tcaaattttc tcgatatgta gactactaat ctaagatttc
    36421 tttctattaa aattgacttt acactttcat ctgtttctag cttttgtaaa agctccattt
    36481 cttcttctgg tttcaatgga ggaggaagta tatttgcccc tcccatatag tatattccct
    36541 ttggctttat tatttttaat ttaataccta acatagtaat aaagcttatt attctttctt
    36601 tcaatcgtaa cattccttgt cccccttaaa ttttcaccta aaatccaggc aatatatttg
    36661 gattcattat tgctccatat tctctatcat taaaatttga aataccaagt attatatttc
    36721 ctatcttttg ctcatctact tctatatagt cagcttttaa gcctattatt atgcttgtct
    36781 tattacttcc agcatgttta taaggtatta ttctaactct acctgaagtt ttatcatcta
    36841 aacttcctat aatttcttgt gctttcttca tatctatatc ttcataatta atatttaata
    36901 gtttttctgg aataaatcct tctaacacac tagacttaac tattattaca tcactttgac
    36961 ttattgggtc ttttaacaaa ttaccactat ctactaaagc tttacagcaa aaactttttt
    37021 ctagtaagtt tatatttata gtctttgtaa gctccttaat gtacttaagc attttcaaat
    37081 ctttataaat gtattttaat aattcacaac tcacataagt acatgcaatt aagaaagata
    37141 tttttaagtg acttattcct gtaaaataaa ttataaaata agtgcttcca gaaataaaaa
    37201 tgtttactag ataaaaaact aacgctacat gaacataatc ttttttgctc ttacttctaa
    37261 atgaaattaa agttattata ctcatgatta ttattttaaa tggtacagta aatagtatat
    37321 tcattgatgg atacaaataa gcaacagaat aggcacctcc taaactagcc cccaataact
    37381 tatttttttt aggattatat cttttagtaa gaatagaagt acaacttata ataatataat
    37441 ttattactag gttttctata acatagtact caatatacac catgtatccc cccttaaaac
    37501 ctttaaacat attataagat agtatgtaca aaattttatg tcacttttga tggtcgaatc
    37561 caaaacaaat tctatttaaa aagaacaatg atatatgtct tatactttat gtcgtcaaaa
    37621 gatatttttt taataccaaa caaatagact tttttgtaga atccaatata tctaggtaga
    37681 agttattcgc tctacttttg gtatatttac aaaaaaaaga ctacataatt taataatata
    37741 tcaagtaaat cttttactta tataaaatca aattatatag tctgtaaatt caaatataaa
    37801 ttattgtgct agtaatccat ttgcatattt tgcagattca tttagtgctt gttctggagt
    37861 tatgtctcca gctatcatca tttgaagttg tttccataaa tattcatata tctctactcc
    37921 atgaggtcca actgtcaatg gtctatacat atgtttatct cttgtcacca tttcttcaaa
    37981 tcttgggtct ccttggtatt tttcaccttt agcaactgga gctctaggta tttctttgtg
    38041 gaaagcttcc attgattctt tgcttagcat atactcaaga agtttaaatc caagttcttt
    38101 atttttacat gctgacatta atgttaaatc atctactgaa gcaaaacttc caactgtctt
    38161 atcttctaat gatgttacat atccccagtt taaatccggg aatgatttat caaatgtact
    38221 tgtagcagca gaggatagca ttatagtaaa tgcagctttt cctggtccaa atacactttc
    38281 aatcatttca ttactatctt ttgataaagc atcttctgga atatactttt tcatatcatt
    38341 tataaagttt gcagctttca agccttctgc attattaaat ttaactgatt ttaaatcatc
    38401 attatatata tctccaccag cttgccataa atacggatac cagttccagt ttaagcttcc
    38461 aaatacttta gaaccccaac cttgtgcaag accccactgg tcgatttttc catctccatc
    38521 agtatctttt gttgcttttt cacagattct tttaaaatca tcccatgttt ttggaggttt
    38581 ttctcctaat ttttctagta tatctttatt gtagtacaat acgcctggat tagcagcttc
    38641 tataggtaaa ccataaatac cacccatcat ttttgcatca tctaagtata gataattatc
    38701 tgaagttcct gatttttcta aataaggtgt taaatcttct accgcaccca tttctataaa
    38761 ttgaggaaac atctctgcat acatgtatcc tatatctgga ccttcacctg cacttattgc
    38821 tgttgcatat ttttctgcgt agttatccca tggtactatc tgaaattcaa tttcacagtt
    38881 attttctttt tcaaattttt caaatatcgg cttccatgca tctgcatcat ggtctgttaa
    38941 tggtgcagac catacaacta atttttcttt accctctttg cctttagaat cttctttttt
    39001 tgaacatcct gcaagaattg atactgatag aatccctacc ataactaaag aaagaacctt
    39061 ttttagttta atcatgctta atcccctttc agactttaat ttgtcttttt cctcaaatgt
    39121 ttacatttaa ttcagattta atattaacaa ataagattac tcttgtctaa tcataaaaaa
    39181 ctatactaac tcctataact atattttttt tctataccta ttagatttaa ttcatactaa
    39241 aaacttactt tttaaatttt atcaatattt aaaaattttt ttaagtctag ccacaaacta
    39301 actatctaaa ttgagcaaaa taaaaagaag tctattgact tctttataag cttacatatt
    39361 atcttcttct tcttaggaat gtaggtatct ccatgctctc atcatcatct tcttcaatta
    39421 tacttgcctt tttaggaggc tctatttttt cctcagcaat tacttcttct tcaatttctt
    39481 ttacagcttg ttttactgtt gtatttaatg aagaacgtat gcttgattta ggtttagtat
    39541 ctaaatctaa ggcaccattt tgtaatcctt caaatcctgt cgcgattact gtaatcatta
    39601 tttcatcacc taaatcttct tttatagaag ctccaaatat aacatttgct tctgggtcac
    39661 aggattccat aactaaagtt gatgcctcat taacttcaaa taatcctaag ttaggaccac
    39721 ctgtaacatt aagaagtact cctttagctc cttgtattga tgtttctaga agtggagatt
    39781 gaatagcttg tcttgcagca tcaattgctc tagtttcacc actagcacta cctataccca
    39841 tatgagcaag tcccttgtct ttcattatag tagtaacatc tgcaaagtcc aagtttatta
    39901 acccttcaac tgctataagg tctgaaattg attgtatacc ttgttttaat acatcatctg
    39961 caactgcaaa agcatctaac attgatgtat ttttttgtac tatctgtaaa agtctatcat
    40021 ttggtatcgt tataagagta tctacttttg atttaagttc tgctattcca ccttcagcgt
    40081 tcttcattct tatcttacct tcaaatgcaa aaggtttggt aactacacct actgtaagta
    40141 tccccatttc ttttgccaag cctgcaacca ctggagcagc tcctgtacca gtaccaccgc
    40201 ccattccggc tgttacaaag accatatcag ctccttgaag taactttact atttcatctt
    40261 tactctcctc agcagctctt ttccctactt ctggatttgc tcctgctcca agtcctctag
    40321 ttaatttttc tcctatttga actttatatt cagcttttga tgtatataaa gcttgtttat
    40381 ctgtatttac tgatataaat tcaacgcctt ttagttgagc ttctaccatt ctattgacag
    40441 cattgtttcc accgccacct acacctatta ctttaatttg tgcacattct tctaattcta
    40501 cgtcaaagtt tagcatcata aacccccttc tctcatgatt caaagttatc gtatgtatta
    40561 attatatcat tttcttcttt tattttgtta acctttaagt aacatattcc acaaaaacgt
    40621 aaattttcct ttttaaattt cttacttata ttgtcaaata ttactcttta agtataaaat
    40681 aatttacgta tatattaaat gctctttaat ttttacgttt tttgttcata atattactaa
    40741 taatatatcg tctaattata gataagttat taaacaatct cataccaaaa actaatacag
    40801 caacatagta aagagaaact cctaactttt ctcctatata agtcaataat atagccaata
    40861 tagcattacc tatgaaacca gtaataaaaa ttacattatc atagttatct tcaaaattgg
    40921 attttatcgc tccaaaaata gaatccatta ttgcaagaat agctactgac atatatatgg
    40981 aataatccat agaatatgta aatggaatat agtaacctac tactacacca aatgctaatc
    41041 ctcctaaaac ccataacata aatcttcacc tctttatttt actttgaaac acaactacat
    41101 tacaatcctt tttattaatt ttttttactt tttctttatt tttttgtatt tttcgtttta
    41161 ttttgcaaat aaattcactt tatttccatt attttcctga acttttatat taattccata
    41221 tatatcctta agagatttag catatgattc tggagagttt atagctgcac tcaataaatc
    41281 tatatctcct atagctttta ttataaatgg ttgtccatat gtagtatcat ttactgtaat
    41341 agtagctcct gaacatttta tcttacttgt tgctagtact ctttctccat ttatagaaat
    41401 tgctcttgct ccagcctttt ttaaatcatt taaaaccctc aaaatatcta tatcatgtat
    41461 aatcaaatcg tttggattct gtccgtcttt taactctctc tcactatccc tcatagtaat
    41521 agttatgcct ggccctgtaa cagttgaata accacttaat tctttaactt cttcaagctc
    41581 tttttgcata agtgtttgga tagattcatt tccattcttt tttgcttctt tatattcatt
    41641 caaagttttt ttgttgtttt taagagtttc ttttaaattt tttatttctt tttttgtatt
    41701 ttctacctca ctttcaatac tttcagtttg acctaatgtt atgtatactc tatttgggtc
    41761 tagtgatttt atataagttg atatgaaaac gcctaatatg aatgctccta atataactat
    41821 tttctttttc atactattat ttcccctctg ctttgtttat atcactatga ccatttattt
    41881 ctatattgtc ttctttggtc acttttactt tggcattata atatgtttct atttcccaga
    41941 caataccata ctttatatta agtgctgaag ccaatgtatc agaatcacca attgctttta
    42001 ttataattgg ttcttttatg acagtgtcat tcatataaag cttgtcttct tttaaataca
    42061 tatatgtatt agaaactatt ctctggtcgt ttatagatat ggcacttgct tgtgctgaat
    42121 ttaatttatt tatcattgat aataataaat cataattata tagtatacta tcatttttat
    42181 cagagtttcc taagtctttt gtagaattta tctcaatttt tatgcctgtt ccttcaacat
    42241 ctgtatatcc agctaatgtt tcatattttt ctatttctga cttaagtatg tcttgttctc
    42301 cttcattgtt tttgcctcta taagtctcta tatctttctt cacactatta acttctgatt
    42361 gtagattctc tttttctttt tttaaagatt taagttcttc tgtaagttgc tctcctcttt
    42421 taaaagttgt catccctcta ttttctatat taataatttt aagttgtaaa ctaacaagaa
    42481 cacccaatac aatacttcct atgattatac ttttatttat aaatcttttt ttcaatatat
    42541 cacttcctta caattgtcta tccttctgga ctatacactg ccaactttcc atagttaaga
    42601 tttatagtcc ctccattagt atttttattt tgcaggtcaa tcagaatttt acttacccta
    42661 gatatattat aatctaagtt atcatcatta gaaagtaaga tttttatatt acttcttgta
    42721 agtatttcta tattgctttc tttttttaag tttacataat ttatttttct ataaattcct
    42781 tcttctttta aataatttaa agttttaaat acattctcct tagttttata atttttaaat
    42841 tttatagatt tattatcatc aatgctataa tctacatcta ctatcaagtc ttttttactt
    42901 tcattagaac ctcttagttc ttccaataaa tttccttttt tatctatata acaataatta
    42961 tcttcatctt ttaatactgc aaatatttct ttttctttta aacttataat tatcttattg
    43021 ggaagttttc tttttatttc cacattttct atatatggat tttttataag tttatttttc
    43081 atatctttaa aattgtatgc aaaaatattt tcatttagat ttacatttag ttctttcatt
    43141 atatttgatt tggtcaccct cttatttcca attacatcta ttttctttac atcaaataaa
    43201 tcacttttta caattataca tatacaaaaa gataacataa gtataaaaac taaaactatc
    43261 atgacattat tcgtatttaa ctttcttctt tttttcaaat ataatcaacc cctttggtta
    43321 ataaatacac ctttagagct tgtttttcct acaaaaaaat tataatattt aaagtcaaat
    43381 ttttgcattt tattacataa catccaaata aggtattaaa ttttataaat aaatcaatat
    43441 ttcatcaaaa taaacatagc atactacata ctatttatat atttaaatct gtatacatac
    43501 aaataaaccc ctaatttata ataatttaga aaatctaata tattataaat taggggtaaa
    43561 tatttactta tcttttcttt attcctatta ccttagcttg accttctata cttggagtcg
    43621 tttctttttt tatttctttt acttccttaa taactttttc tttttttgtt tttttagaag
    43681 ttgacttttg tgttgaattg tatactttca ttatctcatc atatataagg tctatggctt
    43741 caggcttacc tatagtttta cttgcatttg ccatatcaac caaaagttct ctatctccta
    43801 aaagtttaaa tactgctgta tttaggctct ctggagttag atttttttct aaaatagcaa
    43861 ttcctgcccc ttgtttttct atactcttgg cattatactc ttgatgattt tcagcagtat
    43921 aagctttagg tattattata gatggctttc caagagccgt tatctcagct aatgaaattg
    43981 ctcctgcgct acctataacc aaatcacttg ctgcaagtgc atttgccata tcttctaagt
    44041 aaggcacaac tttttggtat ggtttaagat ttatatcgct aatacttccc ataaattcat
    44101 cataatagct tttgcctgta gcaaatataa aagctatatc ttcatttacc atattcttta
    44161 ttacaagtct cattgcatca tttatttttc ttgaacctcc actaccacca tagcaaagta
    44221 ccattctttt ttcgtcactt attgatagat tctttcttgc aatatttttt cttgataata
    44281 aaatttcttt tctaactgga tttcctgtaa aaactaattt atcttctgct gcctctggaa
    44341 atcttttatg cgaatcttca aaacttgtaa gtacctttgt aactgtttta gataaaatct
    44401 tattagttac accaggaaaa gaattttgct catgtattat ggctggtatt ttccccatag
    44461 atgcattaaa taaaacaggt ccactaacat atccaccagt tcctataact atatctggtt
    44521 taaatttctt aacaatcttt cttgattgct ccaaaccttt aaaaagctta aaaactcttt
    44581 ttacattatc gaaatctatt tttcgcttaa agccttgaac tgttacagtc tttaattcaa
    44641 atccatactt aggaactatt tctgactcta ttcccttttc cgtcccaaca aaaattatct
    44701 cagcgtctgg atgctcatct cttattttgt ttgcaatagc tatagctgga tacacgtgcc
    44761 ctccagttcc accgcctgat aataaaactt tcatcaaatc atcatctcct gttaattaat
    44821 tttgacatgc tttgagatgt ttaacactat tcctacagct cccataaaga tggtgagtga
    44881 agttcctcca taacttataa atggtagtgc tacacctgta gctggcattg atgatgtcgc
    44941 aactgctata tttaaagctg cttgtattcc aatttgtgca cctataccta taacaaccat
    45001 acaagcaaat acattagaag tcttcaaagc tattcttaca catctgtaga ctaaaactac
    45061 aaatagcata ataactatta tacaaccaat caatcctaac tcttctccta ttatagcaaa
    45121 tataaagtca ttttgtggct ctggtatata aaaatacttt tgtttactct ttcctaagcc
    45181 tagaccaaac aatccacctg aacctagtgc atatagacct tgtattacct gatacccctt
    45241 accaagaggg tcttgaaaag ggtctaaaaa tgatgtaaca cgactaagtc tatagggttc
    45301 agcaattact aatgcagcaa ataaagcagc cccactccct atcatagcaa atacaatttt
    45361 catatccatt ccagcaacaa atatcattac aaaagtaact agaattacag tacctgctgt
    45421 tgacaaattt ggctgaagta ttatcaacgc aaaaaatatc cctggaacta cgagaagtgg
    45481 tacaacacct tttgtcaaag attttatttt atcataattt ttttcaatta attttgcagt
    45541 taatattatt gtagcaaact ttgcgatttc tgcaggttga aatgttaaag ctcctattcc
    45601 caaccatctt tttgctccgt ttgcttctat ccctaaaggg gtcaaaacta acaataataa
    45661 tactactgct atagctccaa ttgctgtagc atttttcttc caaaaactat aatctattct
    45721 tgatgtaata atcataacta taaatccaag tacagcatat attacattct tttttagaaa
    45781 atagtacgca tcattgtgtt taaatgaaga ttgtataaaa cttgcactaa acaccataac
    45841 tattcctaca aatactaaaa gcatagtagt gtaaaacact actccatcaa attcagtctt
    45901 catccttatg tcaatttgtt ttttcaaatt ttctttaggc atccaactaa tctacctcca
    45961 ttcttccatt taagtaagtt tagaggtaag agattatttt aaattgttta cattatcttt
    46021 gaagtctttt cctcttactt caaaactttc atacatatcc caacttgcac aagctggtga
    46081 aagaagcact atatctccac tttttgaaat ttcataagaa gtcttaactg cttcttccat
    46141 atcttttact atatgtatgt cattaaatcc tttattttta gcacaatttt ctatatttga
    46201 tgcagtttct cctaataaga ccaatgattt aacatatgac tttgcagttt caagaagttc
    46261 atcaaaatta cttcccttat ccatcccccc tgctataagt attataggtc tatcataaga
    46321 ctgtatagct tttatagtgg aatctggatt agttcccttt gaatcattta catatattac
    46381 attgtctaaa tttcttacaa actcttgtct gtgttccact cctttaaatg tcataagtac
    46441 atcctttata acttctaaat caattttgca gacatatgct attgctatag ctgccataca
    46501 attttctaag ttatgacctc caggtaaact tacatcatct ttatttagaa atttgatttt
    46561 atcatctata tctattatta tgtcattatt ttctaaatat actcctccat ttactttttt
    46621 agttcttgaa aagtaaatta ctttggcatt gcatttatca gctaaagatt ttactatctc
    46681 atcatcatag tttaatacac agaaatcctc aacagtttgg ttcataaata tatttgcctt
    46741 tgcttctatg tatttttcca tagtatggtg tctatttaaa tggtcctctg taatatttaa
    46801 tattgcgctt actttaggtc taaattcatc tatactctca agctgaaaac tgcttagttc
    46861 agttactaac acagactctt cacttgaagt ttctatagta tctattacag gatttcctat
    46921 attaccaact acatatgtat ctcgttttgc tcttgaaaaa atttctccta ccaaactagt
    46981 tgtagttgtc tttccatttg tacctgtaat ccctataaaa attggattat tcgatagttt
    47041 aaaagctaat tctacctcac caattatata cttgcctgaa ttttttaatt tcaatatgaa
    47101 tggtaggtct agtggtacac ctggagacac tacaaccata tctatatcat ctacatcttc
    47161 tgggtgatgt cctaatatgt attttatgtc atttatagat ttcaattcat caagtatatt
    47221 tctcaattta ttttcatctt ttatatcatt tacgattaca cttgcaccca atttatctaa
    47281 atgtttaatt gtagatatac cagtttttgc aagtcctact aataaaactt tttttccttt
    47341 taagtccatt tttcattccc cctagcccta aaatactgct attatactta tccaagctaa
    47401 aacaactgtt ataatccaaa aaataaatac aacccttgtt tctggccaac cacactgttc
    47461 aaagtggtgg tgtattggag ccatcttaaa gaatctcttt cttgttgcct taaaatatcc
    47521 aacttgaatc aaaactgata gagcctccgc aaaataaatt cctccaatta taggtattat
    47581 aagcactgag ttagttagaa ctgaaaatgc tacaactgca cctcctaatg ccattgaacc
    47641 tgtgtcgccc ataaacactc tagccggata tgaattaaat cctaagaaac ctaaacatgc
    47701 tccaacagtt gctgctgcta aaactgcgac ttctgtattt ccagcaatag aacttgcaaa
    47761 tagcataaag aatacagaaa ctattaatgt tactcctgat gctaatccat ctaatccatc
    47821 agtcaagttt acagcattta ctattgctac gattataaac atcattattg gtatatataa
    47881 tattccaaca tttatcacaa aatctgtaaa tggtatcata agttgtgaag cacttgatga
    47941 tgatgtatac tgataaaaag caacataaaa agataatgca acttgaagga ttattttttg
    48001 atatgctttt agtccaagtg atctttttag ctttattttt ataaaatcat ctaaaaagcc
    48061 aataaaacca aaacctgcaa tacatataag acctactgcc atatcatgac taacttttac
    48121 tcttgtaagt ccagttatta gaattgcaac aatcattata attccaccca tagtaggtgt
    48181 tccgttttta gctagatgtg tttggggtcc atcatctcta actgtctgac caaatttaaa
    48241 ttttctaagc attggtataa atattggtcc tattataatt actattaaaa acgcaatcaa
    48301 tgcggtgtac gtaagctctg ttattcctaa catcataaaa ctcctctccc ttgtcaatta
    48361 actcattagt ttaagcttat ttatttagat attcgactat tttttctaat ctcatgcctc
    48421 ttgagccttt tactaaaata gtgtctcctg tttttactaa ttcatttaac ttgttaaaca
    48481 catcttctct attctcaaag tgatatatat tggctgaatt gaatcctaat tgttttgctt
    48541 cttctccaat aaacactgaa ttttcaccta tagttattat aatatcagta ttattcatag
    48601 atgattttcc aactaatcta tgaccatact ctgaaatttc acccatttct agtatatctc
    48661 caagtatcgc tacccttcta cctttatatc ttccaagtat ctttaaagca gcatccatag
    48721 aatctggact agcattatat gaatcattaa ttattgttaa tttttcattt tttattatgt
    48781 ctagtctcat ttttgtccct ttaaaatttt ttaaaccttt ttttatataa tctaaagaaa
    48841 tatttagaca caaaccaact aatatagcag acattgcatt atatatatta tgttctccta
    48901 ttgttggtat aaatatttcc tcttttttcc catttattac acatataaat gttaaactat
    48961 cctcttccat agtataactt tcacaataaa catcattgtt tttattaaag ccaaatgttt
    49021 ttaacttata tgcatgttct ttttctttta atgtagataa aaacttatca tctccattta
    49081 caattaaagt atttgtttca tcaaagtttg aagtaatctc catctttgct ttcaaaatac
    49141 cctcttgtga acctagtttt tctacatgtg ataatcctat attcgatatg actgcaattt
    49201 taggatttac tatatccact aaatatttta tttcattgaa tccagacata cccatctcta
    49261 ttacagcaca ctcatgttct ttatttagat taaataaagt caatggaact ccaaaatgat
    49321 tatttaagtt tttttcattt tttagtatat ttaactttgc agatatggca gagtaaatca
    49381 tatctctagt agtagttttt cccacactac ctgtaacacc tataaatgga atatcaaatt
    49441 tttctttata gtattttgaa atatctccta gagccaaaga tgtatctttt acctctatta
    49501 aatttatatc cgaactttct aattttattc cattactttc attttttata aaagttttac
    49561 atccactatc atatgcagac ttcataaatg tatgcccatc taaattttct ccaactatgg
    49621 ctacaaatgc attttgggca tttgcctccc tgctatctat aactatatca cttacacagt
    49681 cattataatc tccatatatt aacttcccat tagttgcaag tactaactct tttattgtta
    49741 agcactccat tattaaactc ctcctttaaa accttagcta tattctctta gcaacaaaag
    49801 gcaatgtata ccgttatata tacattgcct acgataactc ttaactttat aattatatac
    49861 tatatttctg tttttttgaa gatgtttatg ggtatatgtt aattatctgt tactttttag
    49921 ttttcaaaat atagggttat acttgagtct tcattaactt ttactccagg ttttgggaat
    49981 atatccttta cctttgtatc ttctggaagt tctatatctg catctaagtc tggttttaac
    50041 ttcgcatcct ccaatgcctt tacagcatct cctattttta gatttcttac atctggcact
    50101 ttaacttgct tcttttcata ctcagctttt tcttcttctt tatatactgg tttaactccc
    50161 aaatatttta aagagtcatt cattatttct ttcactattg gtcctgctgt agtactacca
    50221 aatgcactta ctcctgttgg ttcgtcaaca atagcaagaa ctactatttg cgggtcatca
    50281 caaggtgcta ttccaacaaa tgaacatata tattttcctg gagcatactt accatctata
    50341 actttttgag ctgtacctgt tttaccacca agtctataac ctggtatata tgctatcttt
    50401 cctccacctt cagttacaac tgattctgca atttctaaca tttttttaga tgtttcttta
    50461 gatataacac ttcttacttt ttttggtttt actgtttcag ttatatttcc tttattgtca
    50521 gtataagatt ttaccactct tggttgcatt aaatctccac cattagctat tgatgatata
    50581 gctgttataa gttgtattgg agttactgat atagattgac caaatgatat tgtagccaat
    50641 tcaactggtc ctacattttt ttcgttatac agtattccct tagcttctcc tggtaaatct
    50701 atacctgtct tatccataag accaaatgat tctatataat catacatttt tccaacacct
    50761 aatctgcttc ccaactcaac aaatactggg ttgcaagagt tttgtactgc ttgtttaaat
    50821 tcttgtgttc catgaggtct gtaatgtctc caacatttaa tctttcttcc tccaacagtt
    50881 acactacctg tacatgtgaa tttttctcca tctttaatta ctccttcttc aagagcactg
    50941 gaagatgtaa taagcttaaa tgtagaacct ggttcatatg tatcacttac tgctggattt
    51001 ctccacattt gataatagcc ttttatttta tctttatcat tatatttttc aagttcttct
    51061 tgataatatg gatatattgg cgttcttgag tcattagggt cataatctgg ttttgatgct
    51121 aaagcaagta tatcacctgt tttagggttc atagctattg cagtaacctt ttttgcatta
    51181 tttaactcat aagctttctg tactgctttt tcagtatagt gttgaattac ttcatctata
    51241 gacaacacaa gaccattacc ttgtacaggc tcataatatt tttccattcc ttgtggtatt
    51301 tctcttcctg aagcatcagt acttactata agttttccag gctttccttt taacttttta
    51361 tcatattgca tttctacacc agatatacct gttgcatctg aagaagtatg tcctaacaca
    51421 tatggtgcaa aattcccata tgggtaatat ctttgattat cttctgccac ccatattcca
    51481 cttaatttag catctcttat ttgacttgcc ttatcatcat ctatccatct ttttactttt
    51541 acaagagcca tatttttttt gcttattaaa gcgtaaatat ctttataatc ttcatccaaa
    51601 atctcagcta ccttttcggc tgcttctttt ttatcttcta cttccacagg tttacaccaa
    51661 acagtatact tagttacact tactgctaac tctttcatat ttctatcata tatagttcct
    51721 cttttgggtt ctattggtat atctcttgtt tgttgttcta gtgcttttgt actcaaccaa
    51781 tttcccttca taagttgtag atacccagtc ctaataacaa ggcaaaaaaa caatgcacat
    51841 gctagaataa gaacgagtac cagccttttc ttacttatcc tctttacttt tctcaaatct
    51901 attcccccta gttatccaca tctatatact ttatttgtcc atcttttgga tagtccatat
    51961 tgagcaattc cttagccttt agctcaattt cttttattga tgttttttca gtttcttttg
    52021 cctttagttc ctctaatact atctcttttt ttcttaaatc tttttgtaaa taatagatat
    52081 cgtacttcat ttccgagatt tttgaataac cacatagatt caatattatt actgaaaaag
    52141 ttgcaatcag taaaatgcat ttagctccta tttttttctt atttctcttt ttataattat
    52201 tctttttttt atttttatat tcatttagat tatgtatttt ataatttttt ttcaaattct
    52261 caccccgata gtatatagtt taaattttcc tctatactat atttgttatt aatcatattt
    52321 cttcctctaa aatttcatcg ctaaattaat acactattac acaatctaat tgtatttttt
    52381 tctatacaca acaaaaacct ataaattcac ttatactttt atatgaatct ataggttaga
    52441 tattaatttc tatttatttt tatcacttct agtattttta gataatttct tccataaata
    52501 taatatgtaa ctaactatct actctctgat tcctaagttc tccattatat tacttaatac
    52561 catctatatc tatattttta tatctaaatt ttcattttat atctcaataa caaagaacat
    52621 ttcttatagt gttaattgtt ttttaattgc tttaccagtg caacaaaata tataatactt
    52681 tttaattaat tataataatc ttatatataa gattattctt tagccttata actacttata
    52741 ttttttctgc tactcttagc ttagcacttc ttgaacgtgg atttacttct atttcttcct
    52801 cactaggtaa tataggtttt ctagttatta ttttcacctc agattcttta tcacattggc
    52861 atataggtaa atgctgtgga catatacaat ctgatgctaa atgcttaaat gcatttttca
    52921 ctattctatc ttctaatgaa tggaatgtta ttatacatat tcttccacct tcattcatta
    52981 tagaagaagc atcatttatc atctttgtaa ttactccaag ttcattattt acttctattc
    53041 gtattgcctg aaaagttctt ttagctgggt gaggtccatc tattctagct ttcttaggga
    53101 tagctttttt aattacatct actaattctc ctgtagtttc tattggtttg tttgctcttt
    53161 cttcaactat aaatttagct atacgcttag cccagttatc ttctccataa tcttttatta
    53221 tcttagttaa ttcatcttct gtataagtat ttacaacatc atatgcagaa aactcacacc
    53281 taacatccat tctcatatca agtggtgcat cttgcatata agaaaatcct ctatcagctt
    53341 catcaagttg atgagatgaa actcctaaat cagctaatac accatcaatt ttgtaaactc
    53401 ctattttttc taattcctct tttatatttt caaaattgct gtgaacaaac ttcactctac
    53461 tttcatattc actcaatctc tcttttgctg tgcttatagc atttttatct tggtcaaacc
    53521 ctataaataa tcccttatct gacagttttt ttactatttc tttagaatgt cctgctcctc
    53581 ccatagtaca gtcaacatac acgccatctg gctttatatt taaattctct atacactcat
    53641 ttaaaagaac tgatacatga tgaaattcca tagttacctc ccttaaattc actttttaaa
    53701 ctattcttca ctaatattat ttttattttt ctttgataaa taaacatgtg caattatgcc
    53761 accaataact ccaattagac ttataacttg tgccattcta agtggtccta acattaaact
    53821 atctgttctt aatccctcta taaaaaatct tcctattgaa tataaagtta tataagtaac
    53881 tataacttga ccttcatatt ttttattttt tctaaatagt aataaaacta taaatattcc
    53941 aaaatcccat atagactcat ataaaaaagt tggatgtacc tttactccat caaccattat
    54001 tccccaaggt aaattggtag cacctccatg tgcttctcca ttaataaaat ttccccatct
    54061 tccaatagct tgtgctaaag gcattccaag tataactgta tcagccattt ttaagaagtt
    54121 aatattttta atcttagtgt atatataacc agctaatatt ccaccaatta accctccatg
    54181 tatagcaagg ccaccacctc taaaattaaa aatttgagac atattttgag catagtaact
    54241 ccaattaaat acaacataat agattcttgc acagattagt ccaactggaa ttgctattat
    54301 agctatattt aatacatcat cttctttaat tcccactctt tttgcttctt ttattgctat
    54361 taaagttcct aaaatcattc cacatgccat aagtattcca taccacatga tgtctatgcc
    54421 aaacagtgta aatgctactc tatccataac gtcacctctt tgtattaatt gaaaatattt
    54481 ccaaagcaat atgctataat tacaattaat tgtaattata ctaaattatg ataacacatt
    54541 aatgttaatt ctacaaaaaa tattaaaatt tattctacaa ctccctacac tatattatat
    54601 ctaattacta taaaattttt taaactgaat ctctcatgac taaagttaca agtgtacata
    54661 gatatttttt aatcaaaaag attgatgcat atatacatca acctcttcta tttcttatct
    54721 atagctctta aatttttacc tcaatttata taaatatatt ttataaattt gttttaccaa
    54781 aataaacatt taaaaacata aactaatact taattagtta ttcttattat gattaagttt
    54841 ctatttaggc tctacaatag aaataacaca atactcttct ttaaaatgct cttttaatct
    54901 ttcttcaact tcctcaaaag taatatcttt aataatatct aaataatcca atagatttat
    54961 atccttaaaa acataagata taaaactatt acctataaag tttatagaat caaagcactt
    55021 aataaatgac cctatttttt tctttttagt tctctcaaac tcttcttttg ataatccttc
    55081 tttttttgat ttttctatat aatcaagaat tatttctttt acttttttag gctctttaga
    55141 atctccagct attatgctaa aagcataatc cacttgagac gaaaatccag ctccaaaatt
    55201 ttcatttatt aaaccttgca tatacaaatc ttcataaagc ttacttcctt ttttaaataa
    55261 catccctaca agtatatcag ttacaatctc tttacgtaaa agttctttcc cttttaaacc
    55321 aacattacta tctttaaaac ctatgttgaa cataggcata gatataggga atttttctat
    55381 aacctctttt tcttttacac cttctggttc ttctggataa aatctttcta tactcttaga
    55441 gagtttgtct actttgtaat tgtttgactt tttagttaca tctataactt tttctacatc
    55501 taagtcacca acaacaaata aagccatatt tcctggatta tagaaagtat tataacattt
    55561 atatagctct tccttagtta tcttatatat actatcaaca gtacctgcta tatcaattct
    55621 agcaggataa tttacataca ttgccttcaa gcaattaaag tatacattcc aatcagggtc
    55681 atcattgtac atttttatct cttgtgctat tatacctttt tccttttcaa cattttcatc
    55741 tgtaaaataa ggtgtttgaa catagtcaat tagatgttct aaactctcat aaaaattttc
    55801 agtagcagaa aataagtagg ctgtcattgt aaaattagta aatgcatttg catttactcc
    55861 taattttgaa aatttatcaa atgcatctcc tccatcaggt tgctcaaaca tcttatgctc
    55921 tagaaaatgt gcaatacctt cattcactct aattttctta tcttctccaa ttggtacaaa
    55981 ttctaagtca tttgaaccat aattagtagc caaaatagca tactttttca taaaacccct
    56041 tttaggcata aaatatacat ctaatccatt ttgcaatttc tcataataaa cttcttcttt
    56101 caatatatca ttaactattt tttccataga catctcctcc attaatttct caagaagtat
    56161 acagtatcaa gctctatatt ttttatagct tcaactatat ctttttttgt aaccttttct
    56221 atactagata ttatatcttg tacttcagaa tttgtctttg ccatagattg agagaaaaca
    56281 aaatcagaca taccacctat attatcagtt atagatttca tagaattaac taaagcaagt
    56341 ttactgtttt ctaattcttc atcagatata tcacctgctt ttacactttc tacttgtttt
    56401 tttatcaatt ctacagcttt atcataatct ttagtttcta taccagatga tataaacatg
    56461 atacttttat acttctctat tgaagagaat atatagtaac ataaactttc tttttctctt
    56521 acattaacaa ataatttaga gtgtggacca ccacctaaga cattgctccc aactactaaa
    56581 gcataatatt tatctatgtc tgcataatcc acatttgctc tataacccat aactaactta
    56641 ccttgagtta tttccatttg ttcatctata acttttacct catccacatt ttttataaaa
    56701 tctgctcttg gtatatttat aatttcttct ctttcaaatt ttagattttt acttattata
    56761 tctacaactt tatcttcatc aaaattacct tctacaacta tatctatagg tgatgtttta
    56821 agtatgtttt tataatgttc atataacttt cttgaagtta tactatctat ttcatcttca
    56881 taaccaaatt cacttacact atatctctct cctttacaca tagcctcaat acatttatct
    56941 tgcgcatatt cctttttatc atttattatt gattgtatct tttctcttaa attctctttt
    57001 tcaatattta aatattcttc tttaaaccca ccatctacaa caagcgtatt atttattact
    57061 tcattaaaaa attctactac ttcttcaaat atactctcat ctaaatactt ttcacttatg
    57121 ttaattactt taaaactcaa tacttgacgt tctccacgtt ttacagcatc agctcccata
    57181 gatgaaccat ataaatcatc taatttattg gatattgctc tagcactagg atatttagcg
    57241 cttccacttg ctattattga tggtattaga gcattttttg tagtttcttt ttcatccaat
    57301 agtctctgaa catataaact tataagattc gatttaaact tttctgtttt tatcaaagta
    57361 agattaatat tattacctaa atttattatt tttttagtat taaccatatg tacctcctat
    57421 ttttatttaa acatcaattt taaaattgaa atttatttat tttttaatta catatactca
    57481 catataaaaa ccttctattt aattatatcc tttttattat tttctaaaag caatattttt
    57541 atttaaatta aatacaaata ttatttaaat caatatgctt taaagtctag tttacaagct
    57601 tgatatatat gatgtataat caatatgtat gtctataatt aaaacaattt tgcaatccta
    57661 tagattgatt gtttaaagtt atataaagaa tataaaaaag aataagagag aggacgatta
    57721 atatgaaatt aggtatagtt ggtttaccaa atgtaggtaa aagcacacta tttaacgcaa
    57781 taactcaagc aggtgcagaa tctgcaaatt accctttttg tacaatagac ccaaatgtgg
    57841 gagttgtatc tgttccagat gaaagattaa acaaactaca agaattatat aattctgaaa
    57901 aaatagttcc tactgctata gaattttgtg atatagcagg tttagttaga ggagcttcta
    57961 aaggagaagg tttaggaaat aaattcttat cacatataag agaagttgat gcaatagttc
    58021 atgttgttag atgttttgag gatgaaaatg ttgtacatgt agatggttct gtagacccac
    58081 ttcgtgatat agaaactata aacttagaat taattttctc agatatagag attcttgaaa
    58141 gaagaattaa taaaactcaa aaagctgcta aggctgataa aactttaggt tctgaacttg
    58201 atttattaaa atcaataatg tctactttag aagaaagtaa gtgtgtaaga actatggaat
    58261 ttacagaaga tgaacaaaca tttgtaaatt cattggattt acttacatct aagcctgtaa
    58321 tatatgcttc aaatgtatca gaagatgatt tagctgataa tggagaaaat aataaatatg
    58381 ttcaacaagt taaagcattt gcagaaacag aagatgccga agttgttgta gtatgtgcac
    58441 aaatagaagc agaaatttct gaacttgatt ctgctgaaga aaagaaagag tttttagaaa
    58501 cattaggtct tgaacaatca gggcttgata agcttataaa atcttcttat gctttacttg
    58561 gtttaatttc attcttaact gctggtccaa aagaagttag agcatggact ataaaagttg
    58621 gaagtaaagc tcctcaggca ggaggaaaaa tacattctga tatagaaaga ggatttatca
    58681 gagcagaaac aatagccttt aatgatttgg ttgagcatgg tactatggct gctgctaaag
    58741 aaaaagggtt ggttaaatta gaaggtaaag aatacatagt taaagatgga gatgtaatat
    58801 tatttagatt taatgtttaa aatgattaat cgtatacact ttgactatta tatataataa
//
LOCUS       DAMKMI010000008        53554 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMKMI010000008 DAMKMI010000000
VERSION     DAMKMI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53554
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.008"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            288..686
                     /locus_tag="PC429_000472"
     CDS             288..686
                     /locus_tag="PC429_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3845996.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(903..1112)
                     /locus_tag="PC429_000473"
     CDS             complement(903..1112)
                     /locus_tag="PC429_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3845997.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1144..1353)
                     /locus_tag="PC429_000474"
     CDS             complement(1144..1353)
                     /locus_tag="PC429_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3845998.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1652..2071
                     /locus_tag="PC429_000475"
     CDS             1652..2071
                     /locus_tag="PC429_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3845999.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2299..2754
                     /locus_tag="PC429_000476"
     CDS             2299..2754
                     /locus_tag="PC429_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846000.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(2961..3221)
                     /locus_tag="PC429_000477"
     CDS             complement(2961..3221)
                     /locus_tag="PC429_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846001.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(3484..3837)
                     /locus_tag="PC429_000478"
     CDS             complement(3484..3837)
                     /locus_tag="PC429_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846002.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANVEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3854..9175)
                     /locus_tag="PC429_000479"
     CDS             complement(3854..9175)
                     /locus_tag="PC429_000479"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846003.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(9192..10175)
                     /locus_tag="PC429_000480"
     CDS             complement(9192..10175)
                     /locus_tag="PC429_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF3846004.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(10176..10874)
                     /locus_tag="PC429_000481"
     CDS             complement(10176..10874)
                     /locus_tag="PC429_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF3846005.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(10871..11923)
                     /locus_tag="PC429_000482"
     CDS             complement(10871..11923)
                     /locus_tag="PC429_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF3846006.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(11916..12344)
                     /locus_tag="PC429_000483"
     CDS             complement(11916..12344)
                     /locus_tag="PC429_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF3846007.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(12344..12670)
                     /locus_tag="PC429_000484"
     CDS             complement(12344..12670)
                     /locus_tag="PC429_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF3846008.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(12686..14215)
                     /locus_tag="PC429_000485"
     CDS             complement(12686..14215)
                     /locus_tag="PC429_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3846009.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(14497..14919)
                     /locus_tag="PC429_000486"
     CDS             complement(14497..14919)
                     /locus_tag="PC429_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846010.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(14919..17372)
                     /locus_tag="PC429_000487"
     CDS             complement(14919..17372)
                     /locus_tag="PC429_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846011.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(17372..17539)
                     /locus_tag="PC429_000488"
     CDS             complement(17372..17539)
                     /locus_tag="PC429_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846012.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(17566..18012)
                     /locus_tag="PC429_000489"
     CDS             complement(17566..18012)
                     /locus_tag="PC429_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3846013.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(18126..18554)
                     /locus_tag="PC429_000490"
     CDS             complement(18126..18554)
                     /locus_tag="PC429_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF3846014.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(18568..19632)
                     /locus_tag="PC429_000491"
     CDS             complement(18568..19632)
                     /locus_tag="PC429_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF3846015.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(19637..20080)
                     /locus_tag="PC429_000492"
     CDS             complement(19637..20080)
                     /locus_tag="PC429_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846016.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(20329..20769)
                     /locus_tag="PC429_000493"
     CDS             complement(20329..20769)
                     /locus_tag="PC429_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846017.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(20874..21071)
                     /locus_tag="PC429_000494"
     CDS             complement(20874..21071)
                     /locus_tag="PC429_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3846018.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            21240..21560
                     /locus_tag="PC429_000495"
     CDS             21240..21560
                     /locus_tag="PC429_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF3846019.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            21642..22148
                     /locus_tag="PC429_000496"
     CDS             21642..22148
                     /locus_tag="PC429_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846020.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            22849..23604
                     /locus_tag="PC429_000497"
     CDS             22849..23604
                     /locus_tag="PC429_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3846021.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(23747..24175)
                     /locus_tag="PC429_000498"
     CDS             complement(23747..24175)
                     /locus_tag="PC429_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF3846022.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(24596..24796)
                     /locus_tag="PC429_000499"
     CDS             complement(24596..24796)
                     /locus_tag="PC429_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF3846023.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(24979..26148)
                     /locus_tag="PC429_000500"
     CDS             complement(24979..26148)
                     /locus_tag="PC429_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF3846024.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            26384..27205
                     /locus_tag="PC429_000501"
     CDS             26384..27205
                     /locus_tag="PC429_000501"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF3846025.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRHEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(27281..28669)
                     /locus_tag="PC429_000502"
     CDS             complement(27281..28669)
                     /locus_tag="PC429_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF3846026.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(28804..29562)
                     /locus_tag="PC429_000503"
     CDS             complement(28804..29562)
                     /locus_tag="PC429_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF3846027.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(29564..30280)
                     /locus_tag="PC429_000504"
     CDS             complement(29564..30280)
                     /locus_tag="PC429_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF3846028.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(30282..30989)
                     /locus_tag="PC429_000505"
     CDS             complement(30282..30989)
                     /locus_tag="PC429_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF3846029.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            complement(31105..31641)
                     /locus_tag="PC429_000506"
     CDS             complement(31105..31641)
                     /locus_tag="PC429_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF3846030.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(31743..32813)
                     /locus_tag="PC429_000507"
     CDS             complement(31743..32813)
                     /locus_tag="PC429_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF3846031.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(32806..33864)
                     /locus_tag="PC429_000508"
     CDS             complement(32806..33864)
                     /locus_tag="PC429_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF3846032.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(33851..34183)
                     /locus_tag="PC429_000509"
     CDS             complement(33851..34183)
                     /locus_tag="PC429_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF3846033.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            34382..35503
                     /locus_tag="PC429_000510"
     CDS             34382..35503
                     /locus_tag="PC429_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF3846034.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(35631..37529)
                     /locus_tag="PC429_000511"
     CDS             complement(35631..37529)
                     /locus_tag="PC429_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF3846035.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            37844..38620
                     /locus_tag="PC429_000512"
     CDS             37844..38620
                     /locus_tag="PC429_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF3846036.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(38712..39464)
                     /gene="xth"
                     /locus_tag="PC429_000513"
     CDS             complement(38712..39464)
                     /gene="xth"
                     /locus_tag="PC429_000513"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF3846037.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(39625..40575)
                     /gene="galT"
                     /locus_tag="PC429_000514"
     CDS             complement(39625..40575)
                     /gene="galT"
                     /locus_tag="PC429_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF3846038.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            40960..42156
                     /locus_tag="PC429_000515"
     CDS             40960..42156
                     /locus_tag="PC429_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3846039.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(42268..43608)
                     /locus_tag="PC429_000516"
     CDS             complement(42268..43608)
                     /locus_tag="PC429_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF3846040.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(43747..44499)
                     /locus_tag="PC429_000517"
     CDS             complement(43747..44499)
                     /locus_tag="PC429_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3846041.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(44647..46224)
                     /locus_tag="PC429_000518"
     CDS             complement(44647..46224)
                     /locus_tag="PC429_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3846042.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGIIAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(46636..47088)
                     /locus_tag="PC429_000519"
     CDS             complement(46636..47088)
                     /locus_tag="PC429_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3846043.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(47399..48844)
                     /locus_tag="PC429_000520"
     CDS             complement(47399..48844)
                     /locus_tag="PC429_000520"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF3846044.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(48988..49896)
                     /locus_tag="PC429_000521"
     CDS             complement(48988..49896)
                     /locus_tag="PC429_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3846045.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            50153..50977
                     /locus_tag="PC429_000522"
     CDS             50153..50977
                     /locus_tag="PC429_000522"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF3846046.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            51017..51547
                     /gene="lepB"
                     /locus_tag="PC429_000523"
     CDS             51017..51547
                     /gene="lepB"
                     /locus_tag="PC429_000523"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF3846047.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(51629..53410)
                     /locus_tag="PC429_000524"
     CDS             complement(51629..53410)
                     /locus_tag="PC429_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF3846048.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT        17629 a         8776 c         5933 g        21216 t
ORIGIN      
        1 tcttttacag acaatcaaaa agcaatctta gtttatcgaa ataaatttat acaatctatt
       61 catacacccc tttacattta tataacaatt aaacctattg ttatatatac aaatcaaaat
      121 aaaccatata ttttcaacta taatatgtta aaatcatatt atgagcatat taatttaagc
      181 aaaatgtcat ttagacttta atttagtagt tttatattat aaatttatat atttttaatt
      241 ttaaatgtca tttagacatt actcataaaa agaaaggagt gtattttgtg gatactttag
      301 gaaaaagaat tgcttatttg agaaactcca aaaaacttac tcagcgcaaa cttatggata
      361 ttttaaaatt tgaaaactta ggcaagtatg aaacaggaga tagaaaacct aattgtgata
      421 tactaatgtc aatagctgat tattttaatg ttacgactga ctggcttctc tatggtaaag
      481 agaaggtaaa tgtcaatagt agtgtaaaag aagataaaga agattactta catgttacta
      541 atgatgaaat gacgatatta aatctataca gacaactaaa cgaacgtgat aaaataaaaa
      601 tagaaggaat acttgaactt aagatatctg aatataaaga cttaaaaaag cattcctcta
      661 ataataatga agataaaatg gtttaaacat acataatttg atacgatttt acatcatcta
      721 ttaaccttct ttttttgtag tatattacta aaatatggat attggattat ttaaaaacaa
      781 gtatagtcta atttcaagca catttataat atttagatta tcttatccat attttagttt
      841 aagtactttg aaaaatataa tacatctcta tgtataactt atattcctaa gaaatgcatt
      901 actcatgttt ttaattcttc caacatcttt ctatacacaa ttgtacatcc ttcataattc
      961 ccctctttaa gaaggattga acatgtacta gaatatttag ttaaagtatt taatctttcc
     1021 atatttaagc tttttatatt actgagtgct gtaataaaat catcaattgc tctacaacac
     1081 tctataaccc catctgaatc caatttacac acaagaatat tcacctactt tatatactat
     1141 ttattaatca tcttctgaat attctatatt aagtaattga taaattgatg atttatattt
     1201 tttacctgtt cttcttccat ttaaaatagc tgacaaatag ttttcattaa cacctatttt
     1261 cttcgctaat tccctttgag tcatatttaa ttcaataagt ctttgcttga cttgcactcc
     1321 caaaaaaggt atccattttt ttcgagcatc catttatcaa cttcctttct aagcgattta
     1381 tatttacttt tatattattt tagcttacgc accccctcta tttagtatat atttttaata
     1441 tgtacacata ttaagtatat aaatttattt aatactaaat aagatttttt gattactttg
     1501 tgctaaaatt aggatgtaaa ttcgtatcat taagttaatt ataaagtcat tttgacctta
     1561 agtcaatggt ttttatgtca ttttgacctt taatttgaaa ctatttacat cttaaaaagt
     1621 ctttttgaca ttaattgaat ggagagtgtt tatggatact ttaggagaaa gaattgttta
     1681 tttgagaaaa gcaaaaaacc ttaaacaata tgaactagaa gaaatgttgg gctgtgacaa
     1741 cttaagtaaa tttgagagaa acattagaaa accaaattat gaaatattaa aatctatagc
     1801 tgagatattt aatgtttcag tagactggct attaaatgga gataatctat cacataaatc
     1861 agatttaatt tgcgattcct cctcaaacta tccacttaat agcataaact ctaatgaaat
     1921 aaagctttta aacaatttta gaaaattaaa tgattatgat aaagctaaaa tagaaggtat
     1981 gattgaatta aaacttcatg aatatgaaaa agagaaggat ttaggaaaaa ttgagtataa
     2041 caaaaataaa gatgagaaaa tagataaata atttttatgt tgcttttgat acgattttac
     2101 atcatttctt tgttctaata tttttatttc atttattttc ttttttataa ttttatgata
     2161 aaataattag tatattttgc tttacttgaa tgtaattatt tatacaaaag tcaaacataa
     2221 tataaatgta gtaaattttt agtttcaagt aatatctcgt tattataact aaatatttat
     2281 taaaattagg aggataaaat gggcttaaga gacaaattcg cgcaatcatt tgctagatca
     2341 aaaactatga gtggccctga gaaaaaggct aatgaaataa tgggtaaatt gcttcttaag
     2401 aaagctatat tacctatcgt tttaatgttc gtgataataa tcgctggagc tatgcttaaa
     2461 atcaattctt gggttacact tggcataaat ttagttattg ctgttggtgc tttcttctac
     2521 ataagaaatt ctagtaaaaa gtatcagaac tttaaacctt atgttggcaa tttgattagt
     2581 ttagagaaaa aaggtaaaaa agagtacgtt gctattataa aacaaggtaa gttaccagtt
     2641 aagttacaaa tagcttatgg tggtgaagac cttgaacatg ttaagaaaaa tcaaatggtt
     2701 caaataagtt ataatccaga tgctaaaata gctattttgg ttaatagaca ataatttaat
     2761 aaaaaaaagg agttgtctta aaattgattt tttctaatca aatttaagct caactccttt
     2821 tttaattttt atatgttatt tttattactt tttttaatta atattttaaa tattaaatcc
     2881 ttctcaataa aatagtgaac tatctacaat actactttat cttttcctaa caatatataa
     2941 tctggtgcta ctctttcatg ttatacacca ttttgtaaca taccttttat atcagtaaca
     3001 tcttccttaa tttcctctac atctgttttc attataccca tttgaacaag tatatttttg
     3061 tttatttctt gttgttgtgt agataattct ataaaatttt ctacagtttt tttatacatg
     3121 tctctatctt cttttttctc ctgcatagta tttttaaata atagagcaca tagtatacct
     3181 attgccccta gactactcaa ttcggttatt aatttatcca ttatagcctc ctattgtcta
     3241 aattttttat acttatagct ttaatttttg ttgtattctt acatcatgca atattatatt
     3301 ttcaggatta tcaatttaat cttctgtact ttaaattgta ggtgtaattt atagattgaa
     3361 taaataataa atatatatac aagttctaat gtaacaaaaa ataagtacaa ggcttactta
     3421 ttaaagttaa gaccttgtac tttacataaa aaaaataact aatacatagt tattctaaat
     3481 ttactattca actggtattt ctaattcttt acatctaatt ctaacttgct ctctaattac
     3541 tgctggtact tcttctattg tttttttgcc tcttaaaatt aaagctacat atacttctac
     3601 cattccactc tcacctccag attttcttac actaaaagtg cttatattac ttgcttcatt
     3661 gacagaactt tgatttaaca acatctcata tatttctaca tttgctataa aattatttgt
     3721 tatatcatca tcttgcttta cttgacctat acttatgtct gaaatgtctt tatactgcat
     3781 ttctactttt ctaccttgtt tgatttgttc tattttattt ttatcgaatt taaacaattt
     3841 tcacacctcc attttaagct tgtggctcgt tgttttcagc atcatatcta aaaatcatag
     3901 atggacgttt tatagctgtc catttatctc ctttttctac tatgatttta ggctgagcac
     3961 tttttatgtt ggcttcttct aattcataga tttcttggta acttgagtct gattttttga
     4021 tttcaactag tttcatgtct ttgaataagt cagatttatt tatttttact tgaactttag
     4081 ttttatttat taattgatat ataggattgt tttccatcat atcatccttt aatccctcat
     4141 ttaatgttac tattaagtca gtgttatttt cagtaatgtt tatgattgaa tatactttat
     4201 tgtttatcat taaaatagaa tcctttttta ctttaagccc tgtttctgat ttaaatataa
     4261 acatacttga atataaatcg cctgtaggac tactggcttt aatcagtatc ttattttctc
     4321 ctaatactat tttattttgt ggtatctcaa atataatgct atttatttcc aaattagtct
     4381 tagtagatat taattcatca tttataaaca tttcaagttt ttctataaca ttattagggt
     4441 cttgtactac aactctcact tgagatattg atttttcaac tatttctata gatgctttac
     4501 ttgtatctac ttttgttatc atgtatattg tatcatctga aagctctata tctaaaatat
     4561 catttagatg ttcaggaatt ggtattttag taaattcata taaatcagta attgatatag
     4621 atattttata catagattta tttttaccag ttatccatag actattatca tttttaatta
     4681 ctagtatgtg gtcatcatta tgtgacttaa tttctttaac attagttaat actttagtaa
     4741 aagttcttat tatcttatct ttttcacttt ctcctagtcc aaataatcca tttgtattgt
     4801 acccttgagc gtacaactct ccctcattag ttaaaacaag tgtcattgag ttatatatac
     4861 ttatatcttt aacagaaggt aatccagtaa ccttttgtaa ctctcttaca tcattattgt
     4921 ggccaactcc caattggtac tcagtattct ttccatatga ccatagagta ttatctgtct
     4981 taataatata ggagtagtta tctccacaag caactttttt gacatctgat atatttactt
     5041 cagtaagagt taataattct atatcttcaa gggtttgatt aatttcttta tatgaattac
     5101 ttcctattcc atataacttg ccatccttag caacagccaa tgaatgtgtt tttgatatat
     5161 caatatcaga tacctcaaat ggcaatatta ttgaagtata cttaaaatta tctttaaata
     5221 tgtttttata atttatatac ttattatttg tttttatata cttatcatta aacattacta
     5281 gcttttcatt tttcttatta aaaactattt tatctgatgt gttatctgtt ttaaaatatg
     5341 aatgtaggga accttcaaga gcttcatttt tatcattatc atatctatgg tgaataccta
     5401 aatgattata tctattatct cctgttgaag aataaaattt tccatatata tctttaataa
     5461 atatagtatt gtaactagaa aatacagtat caacatcttt ctttatagat actttataat
     5521 atggaactga catatcccta taagattcat cagatatacc ataagtatct ctacctgatg
     5581 atatcaaatt gttattatta tctatataaa gtgtattatt agctgttaca aaaacatctt
     5641 taacatcttc cacactattt gtattattat tatttgctat acttccatat ccactaagac
     5701 ctccaacaaa tagtttattg tcatttgtta aaatggctga agtatatcca ttaactacta
     5761 ttttttttgc atttataata ttattaatta ctcgtatccc ctttatatca ttagtctctg
     5821 ttgaaaagaa taccctctct tttttccctg tagtataaat catattgtca tttgttataa
     5881 aaaatgtagc ttcacttcct gctgtcacaa cagattttac attctcaata tttatttttg
     5941 taaatgtact tatacttcct tcagtaaacc caaaatagcc atttacaagt ccacatacat
     6001 agcatgtatt atcattttta acaatgattg atacatcatt acatagatat acatctctaa
     6061 caccatttat aggtatacgt gtaaatatat tcctacttgt agtatcaccc aatcctaact
     6121 gtccttttcc attattacct gctccatata aaagaccatc attagttaat aatagagatg
     6181 tgtttccata tacagctaca tattttacat tttcaacatt tattttagta aatatatttc
     6241 tatttacatt atctcctatt cctaactgac cagaattatt taaaccaaca gcataacagg
     6301 tattatcatt ttttatcgca aatgcatgtt tactattgtc agtaaatcta tttacattta
     6361 tagattttat attatcaata tttattttag taaatacctt tctatttatc tcatctccca
     6421 ctcctaattg acctacatca tttaaaccaa cactgtaaag catattatta ttagttaata
     6481 aatatgtaga ctttgaatgg ctatatattt cttgtatatt agaagatatt tcactactgc
     6541 tattcttttt attgtaattt atcttattat ttaaatttaa actactatta actgatatac
     6601 catgcgtacc cttagagtta tatccacata taaataagct cttatcagtt aatattgcaa
     6661 atctagtttt atctctactt aaatcataag gaggtataaa tatatttttt atatttttca
     6721 ttaccttttt ataattagtg gcattatatc catcttgtgt atctgaaaca tctagttctt
     6781 ttgcactttc agatatattc ccaaaaccat atagcgtgtt agaagataaa tacaatagag
     6841 ctttatataa aaatatggct ttatcagagg taataggacc tgaatttata ttagatgctg
     6901 caatattatt agatgattga ttgttaagat aattccttcc atattgatgt atataagatg
     6961 aacctccaga aataagtaaa ttatctggat tgacattttt taaattactt gacgattccc
     7021 cattatataa ataagcaaaa gttgtagaag atgatgattg tgtacatctt gtaggataat
     7081 aattatatat ataagaatca agatatcctg catatgaaat tccctgatat tgaactctat
     7141 agcttgaatc agttggtttt gtccatgaag taccatggta tccagttaga aaagatccaa
     7201 tgtttgtaca aaatacagtt acatactcat atatgataat attattaatt ttagtaatat
     7261 tattcgtgca tgaaactcta ctataacttg accctgatat atttatataa taccaatcac
     7321 ttaataaacc tagatttaca ttagatccat cagaagtact aaaacctaac aggtaatttg
     7381 tatttatacc agttgcatat accttttttt catcaactac aataaatgtt gcattatatg
     7441 aactataagc atcagttaca ttactaaggc taatctcagt atattgactt attaatgcat
     7501 tactaagtcc attaacataa cttgaactct ttccagaagt gtatatttgc ttattattag
     7561 ttacaattaa tgaaaaccag tttgataaat cagtatttat aacaactttt tttacattat
     7621 ctatagatat tttcttaaat acatctgaat tagaaggaag aacacctgct attgagttac
     7681 tatttggtcc agaataataa caagtatcat catttttaat gtagaatatt cctccagaaa
     7741 tattttttga tgctactata gactttacat tagagatact taattttgtc aactcattta
     7801 attgacttgg taagtcagaa cccattcctt tgtatctatt atttcctaga ccatatacat
     7861 ctccattccc taaaataacc agagtacaat aaggactaaa aacgacatct cttacagaat
     7921 gtatatctag tggaagttca atatccttta tttccatttc ttcttttaat aaagttaatt
     7981 tatcctcaga tttaattaaa tttatataat tactataact atatttttca ctatccattt
     8041 tcttaaattc tacaatgtgc atatcatcaa aaaacgaacc taattgtcca taagtattat
     8101 ctcctgtaca atatgaatca ttatttgaat caattagtat gctatgtgat aaaccaatga
     8161 aaatattctt agctacaata ttttttaaat ctataaatga attatctata ttctcagagt
     8221 aatttgctat acctaactga tatatatcat tatttccagc aacctttaaa gtacctttgt
     8281 tatctataag catagtgtta ccatgaccac atgcaatagt ttttatattt acttcttcta
     8341 tcttagtaaa tagtgatgat tgaagtgcat ttccaaaaca tttttccttt ccacaaccat
     8401 atatatcatt attatacgtc attaacacag tgtgttcaga accacaagct atagtcttaa
     8461 catcattaat agaaactaaa ctaaataaat ctctattatt cttatctcct aaacctagtt
     8521 gaccttgatt attaaaacca gtagaatagc atttattgtc attctttatt ataaaagctg
     8581 atttattata tgaaaataca gcctttacat tgtcaacatc tacttttgta aatgtattta
     8641 catttgcaga tgctccatta gttcctagac ctaattgacc atacaaatta tttccagcaa
     8701 catataaagt tccatcattg tataaaagat atgttgattg aagtccacat gcaatatcct
     8761 ttacgccttt tatttctgga accttctcaa atgtagaaac tgttttccct actttttcta
     8821 atcccaatcc catttgacca tctgtattta tacctgcgac aagtaattct ccatctgaag
     8881 taagtataac tgcatgagat tctccacatg caactttttt aatattggta gatatatttg
     8941 gtattttcgt aaataatttt cttgttgtat tgtcttctcc caatccaagt tgaaagttac
     9001 tattatctcc acatccatac aagatattat tattttttag accaaatgta tagttacaac
     9061 atccagtaaa aaaattaata aagtcataat tatcataccc ttcatcaaat tcatcaacta
     9121 ccatattttt atcgccaaag aaattacctc tttgaagtag tttagttctt ttcattataa
     9181 aaacctccta attatcttat ttcatacgaa tatttattta aagttatatc tgtctgacca
     9241 attgaatatg ccctattaga ttttgtttca acaaagttat tatccatttc atgtatatat
     9301 ctaactctat ttgcattaaa tggtagcacc acattcttaa atgtaacact accaagttct
     9361 ccagttatac actttctatt taaatctaac ttaaggcctt ctatagctat tatgttttta
     9421 gcattagtta aggtatcata ccaatatcca gcttcatcta catctatttc tgactctaat
     9481 tctaagtata ttaagatatc taatactttt tcagataatt tattgtaact attttttaat
     9541 acagatagtt cagtttgtat ttgacctcct attacatcat caatagatat atttttaatt
     9601 ttttctacta cctcatttat agctcctgat aagtttttag cttctgtaga taagagttct
     9661 tcgccaattt ttacttttat ttcttttata tttttatcaa acttatcatc taaatcttga
     9721 atatcctttt gagttacaaa aaccaacgtc gggtctactt ctaatgtaac atttgaagta
     9781 ttggacaatt gtaaaatcat ttttacaacc aaatctttaa tactgccatc ttcagcacgt
     9841 ggcttatacg tctctggata cttacctata gctattatat tattctcatt atccagaaca
     9901 gcagcttctc ttatcataaa tccaccaact gttcctggta aaatagtctc tataactatc
     9961 caattaggat ttttttcatc aattcttaga gaatttatat ttccttccca aactacattc
    10021 tttaaagcag tttgttcctc tgttggctca taatattctc cattactatc tccaacttga
    10081 aacttggtta atccaacaag ctctcctagt gcagttgcat ttgctatttt ttctctacca
    10141 attttggtta atattatata aaatttttct tccaattata ccacctccga tttaggataa
    10201 actactacat tttctacacc tgtatcattt gcaatagcta tattaatctt acaattattt
    10261 tcaatgcatt taatgcttaa aggataaacc tttatttctt ctctacttat cattccagct
    10321 ccacagttta caacagcact acattttata attggttcaa agtatggatg aacttttacc
    10381 tcttcactac ataccatacc tccaccacaa tagaccatac ttttatctag tattattggt
    10441 tctaaatagt gtaataagtg agcaggtttt actctttcaa tcatatcgct acaatctaaa
    10501 agagttttat agtcacagtt atttgctata aaactcaata cgaatgtaaa ttcatcacta
    10561 taaactttta tatctgtttc tgattttgta tatgcctcac atatactttt tataacttca
    10621 atagtactag tacctctgct tctcatttta gcttttatat tgctacgtct tgtgtcaaaa
    10681 tcaagttctt ttttttcaat gcagagtatg tcttcccaca tatctaatcc ccatgttgca
    10741 gtgtcaacaa ataattggtt aaaggtatca tcaatatctt ctttaagaat atttaattct
    10801 gtatcgtatg caccttgtat ctcctctaca atgtaatttc tatcaaatga tggtagttta
    10861 tcaattagct tcattgattt tccacctcac taaaattaac agttgttaca ctaggtattt
    10921 tatcttcatt aatagttata ttatctgtac ttccatttat aagtaggtta cttaaatcgt
    10981 gtacaccagt agtatttata agtattccca ttacttttgt atagattatc tctcctctaa
    11041 tatctctaaa gtatgtattt atactttcta ggaaagattc ttttacattg tctaatgtat
    11101 atccatcttc tagtttcatt actgcactta tacttatttc tattggtaat ggtgtcacaa
    11161 ctgttatagt tggtccaata ggcttctctt catctatatg ttgctgacac ctttcaattg
    11221 tttctgtatc aacagcttga ttatttttcc caaatatcaa gaccttaact gttcccggac
    11281 catcccatct tggataaacc ttaacattat agactccatc tacttccaaa gcccattctt
    11341 catagtgagc tttatttcca cttgtagctt gatttctttg gattttataa aatctttctt
    11401 ttagttcttc atctgtctct atatctgtac caccttcaaa acttcttgtg ttagtaatct
    11461 ttgttactcc agatatatta tcaactagtt taaattcaca atttgtaggt aaattatatt
    11521 tcttaccaac ttccagagct tgaactgggc tattgtcacc ttcttcacta ccaatagtta
    11581 catcttttat tactacaaat agtaaatctc tatatgatat tattgtgcca ttatttatta
    11641 cagtaccttt ctctccaata aattcaactt ctccatttga ctctgtacct aactttctat
    11701 atacaccaaa ttcattgact cttttatcaa gaaactggtt atatgtgtct tgtataaaag
    11761 ccattttatg catctttgaa agttctagat atatcttcga aagttctaga ttatttccag
    11821 ataccatgtt gtttagaaaa gaaccttctc ctttataaat atcaagatta atattttcaa
    11881 gagttctatt ttttattact tcatatgtct ggtcactata catttatttc cacctcccca
    11941 tagatagtgg atacttttac atttgcactc aatatgtctc tgttaaagtt agcacttttt
    12001 acgtttactt ctaatatata tggatttatt agaagggcct cttttatgaa tctactagct
    12061 tcactttctg taagaccttt tgtatatttt tgtcctatta gttctgaaag ctctgtccca
    12121 tattctaaag agtatatctc atgttcatat ctatttgtct ttatacactt gtatatccaa
    12181 acttttaagg cttctttttt ttcaattatt ttaaagtcac ctttttctaa aataggttca
    12241 tctttttcaa aatcccatgc cacttcacga aagattggca attcttctgt tttaggtaag
    12301 atataatcct ctgggacacc tataaaagga aatattgtac tcattataag ctcaccaact
    12361 tacttacaac agcaaatttt tcgcctattc taaacattat taccatatcc ccagattcaa
    12421 agttatctat aaaaggattt tttacttcat gctggtgttc ttgatttgtt tctgtgtcaa
    12481 atgtttctag ctgtctatca atcatccaac tatctatcaa aatatcttct ttttctaata
    12541 tgatgttatt tgtctctatt tttaaatctg gtaatttact tttaattttt ccaataaaaa
    12601 aagaaggttg attatgatac tttccttctt ctcttattat tcctataaat tcatttattg
    12661 gattagccat tatatcacca tccttttata atactctcct agctgtgtta aaatctcttc
    12721 tactacttaa cttacttatt ttaactacat cacctttttg tggagcatgt aaaaattctc
    12781 cattacctat atataatcca acatggctga ctggattatg aaaaaatact aaatctcctg
    12841 cttgaagatt tttttgttct actttcttgc cttttttaga ttgttgattt gacgttcttg
    12901 gcaaactaac attaactttt ttaaaacaat agaccattaa accagaacag tcaaaactac
    12961 ttggtccatt tccaccccat ttataaggtt tacctagatg tttttttgct tctgatatta
    13021 ctattttagc tttatcagat acattaccag aataaccacc aataataatt tttccttttc
    13081 ttcttccaaa tttactagct tcttcaacat ttccaaataa aatatctata tggtatgttc
    13141 cattcttttc tatagttatt gcaggtccta cgtcatttac tttatagact ccatcacgac
    13201 cagaaactcc tgtaattacc tgtatttcat ctccatagtt taataaagga tgtttattta
    13261 aaaactcttt tgtataataa gtttgctcat atttaccaac cataggagca gcgcaagttt
    13321 ttttagatgg gtcaagtttt ttctttctac aatctgtatc tccaccttct tcttttctag
    13381 gacagtaagc tgtaaattct gctgtaaact cttttcctcc tgcataatct tctcccccta
    13441 aattactttc ttccttaggt tcatcctgtc ctgctgactt ttcatccata agattttgaa
    13501 aattaagttc aagctcaatt tgatattctc cattttgcca agtatgtttg tctgtatcta
    13561 tataaaatag tcctacaagc tttgtataag aatctttaac ttttactcct ctaccagtta
    13621 tacaacttac atctccataa cctttaagag aacagctttt ttctatccca ttaaactcgc
    13681 tatcaatatc tacatcttga ttttcttgtt gctgaattac tttttgcatt attacattta
    13741 cttccttaaa aatttcatta tctatttttt cgctaatctt gcttccatac tggtctacca
    13801 ctattacttt attttttaca ttttccatgc tctccgaaaa ggtggtattt ataatattaa
    13861 atccctcttc aaacataaca cttaatgtaa cagttccttt ttcaataaca ttaaacttat
    13921 ctaaattggc ctctatcata tactttttct ttgtcttttt acttgcttct gtatatgcac
    13981 tcattattgt gtcataacca tttactccta taaacatctt tgtatacttg acattggtct
    14041 ttgctattat tccaactgaa agcctatttt cagcaaatac ttgctttgct atgtcttcaa
    14101 ctaacttatc tttaaaattg tatgacactt cactttgtgt aagtaaaaat cccatatctt
    14161 tagatacaaa actaatttca ttactgcttg aatctttaga cctattaatt atcattcctc
    14221 taaagagttc tttctcatct acatagaaac agactgtact agctattggt atatcgattt
    14281 gttggaaatt tacatcaaat gatgattgta ttattgaaaa ctctagtgtc cttgatggag
    14341 atttataatc acctgaccat gataccttgt ctactatgtc agttatgtca tatatacttc
    14401 cgttttttat gtgtacccaa atcttaataa taaaaaatca cctgcctttt catttaattc
    14461 tatcagcagt tatttataac ttattatcta taatagttat tgttttggag ttacttttgg
    14521 tatctttatt cttttatact cttttaaatc taatgtaaaa tatacatctc gagtcccatc
    14581 tctttcttca taactaaacc cttcaattat gacttccata tttatatttg tttccgtaat
    14641 tgtaaatctt aatataaaac cttccttcat ccatttttct atcttattta cacaatcata
    14701 tggtgatgga aaacctgtat agtcacagaa agtatagtct ttatttggaa aaaaacttga
    14761 tattgaaatg cttttaagag ctacaccacc aaaagttgct atttcaccta ttttgagtat
    14821 actagaagag tttacagcag cttttccatt aatactaaag gaagatggaa atactggaaa
    14881 tctaaatcta tcttcagctt gtctaagcca catttccatt ataagacacc ccctaactgt
    14941 gccaattgga tacttgcaac taattttgat gttatctttt ctatatcagc ttcttctcta
    15001 atcactattg tatcagctaa cttagctatg tttatatttc caccattttt tctgcttcta
    15061 tattgatttg cttcttgttt agttaaaacc atttcacctt catggagtct ggtttgatag
    15121 tcattataag gaacatagtt caatccaaat gcattattac ctttcttacc tgaataagct
    15181 ttaccagaat caccagcctt tgttccagca tatggattat ctttgtaatt ttttttactc
    15241 ttttccacta gatttaaaac tgttcctgtg atgcttccat tttttatctt attaatcata
    15301 gtttgccacc atttacatat atctttaact attttacata ctttatctaa tatgttaaaa
    15361 aaagctccca atattggctc tataattttc catccagttt ccaacagagg tcctaaggtc
    15421 tcccatacag ttttccaaat aacacccagt gtctctataa aattgtttat ttgaggtgca
    15481 tattcaccta aaaaagtaaa aattcttgtg ataataggac taacagcttc aactacaggt
    15541 ccgattagtt ggaatgctgt agttactatt gtaccaaata tacttattaa tccttgtgca
    15601 attggtgatt tattgtttat actatctagt aaattattta ctaagtttac tactggaagt
    15661 aatgaatcta ttaatgctgt aaaatcgata ttttgtaata gtgtacttcc tgcgttaaca
    15721 attgcttttg gtgcattccc tatcattgtt gctgcatgat tttcaaaacg ttcttgctta
    15781 ttttcaacca tcattttacg cttttcagcc tcattagaaa ttcgatattg agtagaatca
    15841 gaaaactctt ttccattttt tacacttcta gattgcatct ggtcatattt gttgccaata
    15901 attatcttac cattcattac attgcctatt ttaccagcca ttttttcaga taagccagct
    15961 aaattgccta atccactttc tataaaagtt tttattttct ttagtccttg acctaaaata
    16021 tcatcaaaag gtttaacaat actcttcaag ccattgttaa tgcttgtttg agctcctgat
    16081 attatacctg gtattgtatt actcatttct ttagctaacc ccatctgctt tacacttatg
    16141 ccttccaatg cttcatcaaa agttttataa ttttctccta ccagagtatt aagagattcc
    16201 atgtttcctt ttgctgcact taagaaagct gctgatacat cttgttcact acttgtattc
    16261 atatttaaag ctctaacatc taccatagct tgagttatat ctttttcttt tttgccacca
    16321 gtaatattac taatcatttt agctttagtt cccattgcaa caacatcttc agggtcaaaa
    16381 ggcgtatcat ttgccattct tactaaactg ccataataat catcaacatc tatttgatta
    16441 acttttccag tatttctttt cattacactt tctagaacaa ctttctgatt ttgttcctga
    16501 ccaagtaaat caacaccctt tttcacagcg acacctatag tagcaccatt tgtcaaatca
    16561 gatatcttcc cttttatcga accaagtatt ccacttgcca tatcctttac agctattgca
    16621 ccactccata ccttacctgc aaataatctc aatttgggaa atactttact tataatgcca
    16681 cttgccatat cttttatagc tattacacca ctccaaaaag tatttttaaa caacattaac
    16741 ttaggcaaaa ttctacccat agcactactc accatatctt taagattaat acttccttga
    16801 taaggacgtt tctctacttg ttttaatttt cccaagatac tacttatcac tctcttagtc
    16861 atatcttttg cttttattac gatattccaa atcttgccac caatcttttt taaatttcca
    16921 agtaatttat ttttagtttt ttctacttca tcacttttag ctttaaatat taatataatc
    16981 ataatatttg aattaaactc atttttcttt tgaatcaact tattaaaata atcaataatc
    17041 atagctatag ttattgctag tctcataata cttccattta aacttccaat aactcctgaa
    17101 acagcattta tgctagaact taattttgtc atagttgaac ttacactact agcagtagtt
    17161 tgaacttttc ttaacgagga atttaaactg tttactctat tacttgcact tccaatagaa
    17221 ttttttatgc tactagccat tacattagtg gaaagtgaaa cagccacagt tgctctccta
    17281 aaattatcag ttactgatat aatttgattc atttttggtg tgtaattatc tgtaaggtac
    17341 atatttatac cttcttctct cacatttccc attacatacc acctcctgtc aaaggacaca
    17401 ctccatatcc atgtttcatt ttttcaactt cttcctcaat ttctaaggcg aaaaaagctt
    17461 caataagctt taattcgcct ttatccattg cataaaaaag ggacggtctt ataccttttt
    17521 tcttccaata atagtacatt attgtagtcc tggtatccgt ccctattagt tttttacctc
    17581 ttctatcact gcatttttac cataacccat aacatcttga agtattctat ataagttggt
    17641 aatttcacct ggtaataata attttcttgc taattctttt ggtgttggag ccttaaattt
    17701 atccattagc tccttatttt taaataaaag ctttccatcc aaatcacaaa caccttctat
    17761 aattgtaagc atctgcatct ccggtaagtc tatatccgtt ttcatatttt catcaatttt
    17821 aagacaagta ttttgtattt cttcatcttt acttattgtt aaagctctac acattacttt
    17881 aaattcctgc ccaaatatag ttgataatct cttaagttca actatagtac ttggtctttc
    17941 taagttttct gtatctgaat ttaaaagtaa atctactatg tttatatttt ttgataatcc
    18001 attttcactc attattattt gctcctttat caatcaaatt caatcaacta acttttttac
    18061 ttattaacta tctgaattta ttaagtatat acttactaaa tatcagtata aaactaaata
    18121 taaaactaaa ttatatcaag aaaatcatac tcagtaaatg tgaaaggagc ttctatctct
    18181 ccttttactc caacctccca atcagctaaa gttaaatcat caaatgctat attttttact
    18241 gctattcttt ctgctccata agaatctggg tcatttaatt ttgatatagc aacaaatcta
    18301 ggttcagaac ctctctttat cttttctcca attaacttgt gcattcttga actaacgtgg
    18361 tatagagtta ttgagccttt tcctttatat cccatatact ttgtatcagt acccatttga
    18421 cctgctatta taatatcttc ttttgtaaat tccatctttg cttgaaattt ctttacttct
    18481 gctactttgt ttccatcaag ccaaagttct ccccaagtac cactcattac atttctagct
    18541 tccatatttg ccatattaat aatcctccta tatttctatt gataaatcta tatcttccat
    18601 agcatcaagt acttttattt ttgcttttaa aaatacttta gaacctgtgt tagcttcttt
    18661 tatttcttgt aatgtcatat aagataaatc tactcctttt gattttaaat acgatttttg
    18721 tgcttcaaaa tctatttcaa cagtagaatc agattctata agtgctgatt tttctaattc
    18781 ttctaaataa ctttttatag ctactatcaa taaacatttg ttgtcataac tgttagtaac
    18841 ctttcctata tagtcatcta ttatcacctt tcttatgtca ctatgtataa tatctaaagt
    18901 gtcaactatt tttattttct ggaacatttc tcctttttct gctgttaatt cagttaaaga
    18961 atttactcct ctagcaattc ttatagctcc tgcttcttta ataagtataa gctctccttt
    19021 attaaccctt gattctgcat caactttcgt catcttaggt atatcgacta catcactaag
    19081 tttagtataa gttactgatt gacttaaagg tgtacctgct ataagtccag ccaccctgct
    19141 tgtaaactca tcaacactgt atttcttttc tccaactaac acatcttctg tagtaaaatt
    19201 aattatccct tcatgatttc ctacaacttt tcctaataca gctttaacct taaccttatc
    19261 tatatctcta agtttaatta tccaattttt tatagcagtc ttatcagctt ctactgcttt
    19321 tggcatacat agataattaa attccttagt ctctaaaaaa tctaatgcag tttgaatatc
    19381 tgcttctcct tctattacat aaactaataa tttatttgga gtgttaacat ttcccatcaa
    19441 agctaaattt atatattctt tattttcagc agataaatca actggtatat cctctttttc
    19501 atgtatttca ttaagaccta gtgccttagc atctttaagt accattgcaa ttattcctct
    19561 agctgaacgt tctttaacag ttgtagctag ctccttaaat gatatgttga tacttggtaa
    19621 tcctatagcc atactatcac tctcctaaat ttaaattgat ttctttcata ttttcatata
    19681 ctacatcttc ttcatataca ctatccagat agtttagtgt aaatgtaaag ttcaatacat
    19741 aaattgactc ttctttttta atactttgtt ctagctttgt tatatttaat tttctgtctg
    19801 ttacctttat atttctgtta aatagcttat ttaattcatc tgaaatttca tacattctaa
    19861 tactctttga ctgtggtttt ggtaagtatt ttatagagat aacaatatct ttgttattcg
    19921 tatttaacat tacaggtgta caaataactg atagtatact tacaaaaaaa caagaccctt
    19981 cagagccttg cacactttca tcatcaataa aaatactttc agtaaagttt tttttgagaa
    20041 tctcaataat tgtttctaat atttccttgt atttaagcaa ataatcacca cactatcact
    20101 aaatattctt gcttaatctg ataatcttac tataaatgat ataagtcatt tttaaatata
    20161 tatctcattt attcctgtca agtatcttct atatttttag gtaatattat tcttttcaat
    20221 ctatcacctc cttcaatatg gatttctttc acaataacat tatctcacat attttattgc
    20281 ctattgttgc aatggtgtat ataaagtgtt gtaaaattgt cctatttttt ataatatctc
    20341 actcaattta aatatcatct ttttctttaa attgtagcaa gatgttctat ctaagtgtag
    20401 tttcatagaa atatatgtca tattgttttt tgtcttacta ttataaaaaa gattaaaaaa
    20461 ttccatttct atatcattta gacacgttag agcattttct atcttctctt tttctatttc
    20521 tatatcttct ttttccattt ttaattttct taatctttcc tctcttttta acacctcatt
    20581 ttccacagaa gagcttatgt tatatgtgtt actagtcttt tctgtatata ccattgctcc
    20641 acatccagag tattcattct cacatctttt tatgtccaag ttaatattct ttatctttat
    20701 atccaaatac ttataattat gtaatctttt atctgtttct ttaaataata tatctttttt
    20761 gttttccata gtacttcccc ttttttatat acttattatt agcttcaaat ttacaataat
    20821 ctctttagtg cacagttttt tactaatctc acaactttca agaacatatt gttctataac
    20881 tgtataaaaa aaacttcttc tatattttgt tctaaaatac tagaaatctt cttagccata
    20941 agaagtccag gattttttat acctctttct atttgagata tataatcatt tgtaattcca
    21001 gcctttctgg caagttgaat ttgtgtcata cctttgcatt ttctataata tttcaagtta
    21061 tttttaaaca ttgttttata cctccattac tattaagaat attttgttct tcctcactta
    21121 gattatatag aattatttgt tctttatcaa tagattttag aaaaaaatat tcttttatat
    21181 tttattagtt ttatttttta gaacaatttg ttataatcta tacataaggt ggtgttataa
    21241 tgtttgccaa aagattaaga gaacttagaa aggaatttgg attgactcaa agagaacttg
    21301 gagaaaaagt aggcgtttcc caaagagtct tagggtacta tgaaacagaa aacagatttc
    21361 ctgatgaaca cattttaaat aaattggctg atgtatttaa tgtatcagtt gattaccttc
    21421 ttggaagaac cttagttaag gaaaatattg atacagtagc tgcacataga aaaaatccac
    21481 atgaagagtt acctgaagaa gcacaagaac aacttaatga ttatatagaa tttttactaa
    21541 ataaatataa aaaaaaataa accatatgat gagcagtcta tttttctgct cttttaatat
    21601 aatttaaagg caaacataca ttctatttta gggggatttt tatgaataat ttggataagc
    21661 tttttgaatt agcttctcaa gaagagataa taattcatta taccacttat attgcaggtg
    21721 atttagaagg actatatata aacaaacatg gtattaaaat tatatcatta ctcagtaatt
    21781 taaaacaaaa ctcaaaaaaa ttgacatcca tcttagctga agaacttgga catcatttca
    21841 ctagccttgg atactatgta tcctcttata acgattatta tacaaaaatt attatagata
    21901 agtgcgaaaa taaagcctta aaatgggctt gtgagttctt aattactgaa gaagatataa
    21961 taaacataat taactctggc atcacttgcg tttatgaaat ggcagacata cttaatgtgg
    22021 atattacttt ctttcaaaaa agattagaat ttctatcatt aaaaaaacag tctttacaac
    22081 ttggaaataa taaatattta atattaacta atttgccgta tttctacata tttgatccaa
    22141 tttcataata aaaatagtag taattttaac tggcttaaca gtaaaatcta tatatttatt
    22201 ttaacaaatt ttggtttcta taaattcaat attaatatat aataatgata tcatattatt
    22261 taaaattcta aaaacaaata tatgcttttg ttaagaaagg ttgtttgatg aatgtataaa
    22321 gaaaagttgt ttgataatta ttttaagttc ttagcactat tattttggcc aattatgtgg
    22381 tataagtgga tagtaatttc aaatggtact cttgaaaata tgttatttac catatatgca
    22441 atcgtcgcaa ttgttttcat cattttatat tcggtgttta tgataaaata taaagacatt
    22501 acacaaatag atttctttta caggataagt actctacttg cttttatatt tacacttttt
    22561 agctttttaa tatacccaaa gagtttattt ttcttgtatt taaagattat ttttactgga
    22621 atataccttt attattcaat agtaaaaact cttaaattta aagatgacga aggtgtagtg
    22681 ggtattatga gctcactttt attaattgta ataactttgt tctactaaat ttaattctat
    22741 ttgacttaat atggctttaa atattagaca tctacattat tttctgatat tatttaagat
    22801 ggtagtctta tattaataat gaaatacact atgaaggagt taactattat ggaaaaaaaa
    22861 gttttagcaa cagttggaga aaaagagata actaatattg atattgaaaa tgcattaaaa
    22921 tcattagacc cttatcaagc tatgcaattt aaaactgaag aaggaaaaaa acacttatta
    22981 aatgatttag tgaatcaaga attattcttc ttagatgcta aagaagaaaa attagatgaa
    23041 gaagaaatat ttaaattaga aatgaaaaag atagaagaaa atgtattaaa gcaatttgct
    23101 ataaataaag ttttatctag tgttaatgta acagaagatg aaaaagtaaa attctttgaa
    23161 gctaataaga gctctttctc taaaccagaa agtgcaactg ctaaacatat attagtagat
    23221 agtgatgaaa aagctaaaga aatacttgct caaataaaat cagaagaaat atcttttgaa
    23281 gatgcagctc ttaaacattc ttcttgccca tctaaagata tgggtggtga tttaggaaca
    23341 ttcggtagag gacaaatggt tcctgaattt gaagaagctg tgttctctat ggctaaaggt
    23401 gaagtaagtg aaccagttaa aactcaattt ggataccata taataaaatt agaagacctt
    23461 caagaaagta cagagtctac atttgatgaa gttaaggctg aagttgaaaa aagtttatta
    23521 tatcaaaaac aaaatgaagt ttatggaaat aagataaatg ctcttaatgc taagtacgga
    23581 aaccttgtaa aatacaacga ttaatcataa ggtcatatat aaaaaagctg tcttttcaca
    23641 atatttatta gaaaagacag ctttttatat taaagtttaa atattcagtt tcatattatc
    23701 attcttttat tgtaaagaac tcagcttaaa agtttaaata agatttttaa ttatcattag
    23761 ctaaattttt atatccacgt attatctcat aacagtaacc tattacatct cttcttttta
    23821 ttatcccaat aaatgtgtct tggtcatcta taacaggaat gaaattttga tttaaagatt
    23881 ttataactaa atcttcaata tctgcgttga tggaaacagg agaattatcc atttttctat
    23941 ttatatctgt cactggaaca tcttccaatc cctttaagtc cagatttaaa tcatttttta
    24001 atgtccataa aaaatcacct tctgttatag ttccaacata tttaccatcc ttacttatta
    24061 ttggaattgc agagtatttg tgatattcca ttttttctag ggcttgcctt atagtgtagt
    24121 cttcatatat atatgctact tcactttttg gtgttagaaa gaataatatg ttcattacac
    24181 tacccaccgt tactgaaaac ttataaatat tactcaatat ttataatgtt acttttttgc
    24241 agtaactttc tcctttctta ttttctaagt attcttaata ctatacgctg taacaagcgt
    24301 ttttcctttg ctcattttaa tcttattatg ctttattaat atttttatat ggtctatcag
    24361 tatcacttat tctcttttta gaaaatttac taaaaattaa ttgacttaaa cttagccata
    24421 ataattttac tctacactat taattttaac atatttatct cattattaat gattttattt
    24481 taataatttt aatttatttt gctattaatt ttatttacaa aaactgaaaa aaccgctaaa
    24541 tgattagcgg cttatctctc atattactag aacactaata tatatgaatt tttaattata
    24601 atctaactac gttagcagct tgaggacctt tagctccatc tactacttcg aactcaacgc
    24661 tttgtccttc ttctaatgtt ttgtatccat ctccagttat agctgagaaa tgtacgaaaa
    24721 catctccttc accttctaca gatatgaatc caaatccttt ttcgttgtta aaccatttaa
    24781 ctacaccagt tttcattaat aaatcctcct aaaaaaacac aaatatttag ctactctttt
    24841 ctgtaacctt attactactc tatcatatat agctatgttt gtcaattatt attattttta
    24901 tacattaata atcaattttt ttactttata attttaaatg attattatct tttacacttt
    24961 atttttcttt ataatttttt aataatttat ttttattata tggaatctta tggacttatc
    25021 actccaacct aaatcccatg gcaccaacat tctatcagta ttatgacaag ttaccaaagg
    25081 ataccctctt gagtctaatc ctgtcactgt cgaaacatgt gttattctgc ctttcttctc
    25141 ataaccaaca aaatcccctg gacgtaaatt ataagcttct ttgtatactt cttcatagga
    25201 acctttagca attaatgaac ctcttccact atacagtgca taatttttaa aagcttgtgc
    25261 atttacccat gcttttgtac ctgctctctc gctataattc caaattgagt tctttttgaa
    25321 ccttccactt tcaaacatta tctgtgatgc aaagtttgca caatctccac cttctggatt
    25381 ataatcccta tagtttggat taaatttcat accatgttct tcatcagctg cggctccaca
    25441 gtatttatgt gcataatcta tagccttttt ttgttcatct gtaagctgta actctacacc
    25501 atcttgttga ccaatataat ttcttatatc atcagatttt atattttcta aatttaaaga
    25561 gtctgcaaaa gggtctgtat accactcttt agttatgaca tattgattat ctataatttt
    25621 tacatttaga taatgatatg tacctattct aaaggcattt tctttgtcct tttgattttc
    25681 atatgaatac ttatattcag tcgatactga acagattata ccatagaggt ctttttcttt
    25741 ttcttttatt tttttaatct taacccttgt ctttatgtct ttaaatttta caccttgctt
    25801 actagaccaa ttttctaaat acttcatctt ttttacttca ttttcataag cccacagacc
    25861 aaatttttta tccgtctcat aaagaccttt taaaatgttt tcatttttct ctaatattgc
    25921 cttatttctg taatcaaata agttttcaag caatagttta tattggtctt ttacatcatc
    25981 accttcaaca tttgcagatt ccatgacttt ttcttccata tttttcatac ttaaatcatt
    26041 gattaaaaat acacctgctg tagtagctaa agacaaaaat acaaatgcca ttataccttt
    26101 gatgtactta ttcttatttt tcagtatttt caaagctaca cctcccaaat agtacacctt
    26161 acaaatcaaa ttacaaagaa tattctgtca catatagtaa ttttcatatt taataattct
    26221 ctattttatt atgtccaata ttaaaaatta attgtatcaa tacactgttt tttttacaat
    26281 tttttatttt ataatctact tttttctttt ttttatagta atatatatgt atataaatat
    26341 tttggtatca ttataattaa tacatactaa tggagggttt tttatgcaaa aaaaagtcgc
    26401 agcaattaat gacttatctg gaataggaaa atgctcatta tcggttgcta tacctatact
    26461 ttcagcatta aaggttcaat gttgtccatt tccaacggcc atactttcaa gtcaaactgg
    26521 ttatcctgaa tttacttttt tagactttac cgatgaaatg gtaaaatatt caaatgtttg
    26581 gaaaaattta aaagtcaatt ttgatagtat atatagtggt tttttgggtt caaagcatca
    26641 aattgagata gtggctaatt ttataaacga ttatcccaat gcttttatcg tagttgaccc
    26701 tgtgatgggt gataatggag ttatgtatcc aatattcaca gaagaaatga gacatgaaat
    26761 taaagaatta gttaaacatt cagatttaac tactcctaat ttaacagaag cttgtttctt
    26821 aactggaaat gattatacca aaagcgatta taatagagat gagcttatat atattgcaaa
    26881 atctgtatct gatttgggac ctagcaaagt cgttattact ggtatattag aagatgataa
    26941 tatattaaat ttggcttacg atagagataa tgaccatgta ttctttacat cagtcaaata
    27001 taataattgt tcttatagtg gaactggaga tatattcaca tctatacttt gtggtatgtt
    27061 agtaaataaa catgatttgg gagtagcggt taatactgca acagatttta tatataaaac
    27121 tataaattac acatctcaat ttgatactga tagaaatgat ggagttatgt ttgaaaactt
    27181 tttatcagat ttaactaata tataaagtca aaaaaactat ccattttata aatacctata
    27241 aatcataaaa tttaggtatt tacaaaatgg atattattta ctaaactatt ggtatttcaa
    27301 ttgttaccat agcaccacca gttttcacag aattttcaat atataaaatc ccattatgtt
    27361 tttgtacaat attatttaca atatagagtc ccattccata atgagattta gaatttctac
    27421 tcaaatctcc tgtataaaac tgttctgtag cttttgacaa atctgcttta gaaaaaccac
    27481 ttccacaatc tgtaactaca aattttaagt atttattacg tactgataca cttatgtata
    27541 gtttactctg gcttggtgaa taatctatag catttgaaat tatattcata atagctctga
    27601 ataacagttc tctatctata gttatatttt taggcaaatt atcttgttta taaaactcca
    27661 catctaagtt tttagtacta gctaatgctt ctatttgcac taagacatct tttataaact
    27721 cactcatact gattgcttct ttatttaata tatagccatt ctctgttttt gatatatcta
    27781 ttaaagtatt aatatatttt tctatttgag tgacaccctt tgagatatat tccatgtatt
    27841 ctaataactc aggattttca tttatttcaa ttaataagtc agtatttcca tgtataattg
    27901 ttagtggtgt ttttatatca tgtgccagtg cagatatctg ttcctttcta ttttcttcta
    27961 gcatccattg tttttctaaa gaatctttta acgccaactt cattttatcc atagaaaaaa
    28021 ggacattatt aatttcataa attttactag attctacaac aaaatctaaa tcctgttgtt
    28081 ctattttttc tgtagtattt tttaaaagct ccatttcaat attaaatttc ttgccaaata
    28141 cttttgataa tataacaatt tcaataaaaa atattatact aaaaataatc atacccaatg
    28201 tctctggttt tggtaaatat gctcttaaaa ttggagaact atactctgat actattttgt
    28261 attttattat aaaaacttca tctttcttaa aaaactttat ataactttct gaatatgcat
    28321 gcctttcttc aatatctctc attaaattcc atacttcttt tgattctttt ttattaaagg
    28381 tccctgaaat taaactccca tccaaagtat aaaccccata ctcatatata cttggaatta
    28441 tatcttcagt tactttttca cttgaaataa tctgttcttt atatttatta aattgttttt
    28501 ctgcataatt agctggtaaa attacacctg atgataataa tacagaaaat gataacatta
    28561 aaaggagtac aattgaaatt gtcataataa agaataaaga taaatatttt ataaaaatag
    28621 ttcttaactt ttcaactttt ttgttgttag attttttacc tccaagcatt tgtaccctgt
    28681 cctccaatag tttaattaat tcataattaa tatctaacca gttttttatt tgattatttt
    28741 atatcttttt attgctaagt tatattttaa cataataata taaataaaca gtcaattttt
    28801 actctattct tctgattttt taccttccca tttagaaaac caaatacata aaattacaaa
    28861 tgctaaaatt gttacaaaac cacacattaa acctgttgat tgatatgctt ttattgattc
    28921 aacctttata tattcaaccg ttttagaact cgaaaaagac gaccaaatag ttataaatct
    28981 aacaccccat gcacaaggaa aatactgcca aataccagaa cccaatccag ttaataataa
    29041 tgctgaaagt aacatttcaa ctatacctac tccaattgat gttccttttc cgaaccttaa
    29101 acttaagaat aaatgtaata tgtattcaaa taaattacac ccaattaaaa ttagaattga
    29161 aatcatataa aagcttgatg caaagtaagc cttagatata ccaaatttaa aacctaatac
    29221 agacactaat gttgctacaa aaccaagaca tactaagatt atatatttac ttataagagt
    29281 aagatatttt ggattagatg ttgttaacat atgtttataa ttccctgcat aatattcttg
    29341 ttcaacaaac attgaacaca caacacctga caataaagga aaagctattg ctataacttg
    29401 taaatatgct actatcttta gtatattatc agctcctgaa accttataat accataaaaa
    29461 caatcccatc cccattaaag caatgcaaat gtgcattagt attataaaat ttctctttaa
    29521 ttttattaaa tcagaacaaa atattcttat taactgtctc atattacttc acctctctat
    29581 tattgaacca aacccttgta gctaaagcaa ataatacaaa taaaacaata gatattaata
    29641 ctcctggcaa aataacagca ttatttaaaa attcacttcc ctctggtaca ggaagaccat
    29701 ttggcagaat atgcaataca acagccatta atcttgaagg aatcgaatat ggaaacatat
    29761 accacatttc tgtagttgag aaaacagcaa ctccaacaat agtaaagaac atatttatta
    29821 gaactgtaac aaatatacca aattttgaag ctaaaaacat acaaagagga atctgccaca
    29881 taaatagtaa aataattaat atagttgcaa tgatattttg acctattgaa atagtttctt
    29941 tatagattat cccaacaaaa actacaccta ttgcagaaat aattgatgct ataaaacaac
    30001 aaataataca gtaaatcatt tttccagtcc aaacgtcttt catttcaatt ggcaatggta
    30061 aaataccacg atatttactt ttttcatctt ttaatactat taaagaagat attaatgtta
    30121 atgctcctgg aagaaatgtc gtataccacc agttgaaagc agcattttgc cctccaccta
    30181 gtccacataa aagtatacaa aacactggaa ctatccatat caacttcatt ccaaaagttc
    30241 ctttcatctt ttgaaattcc actctaataa atctttccat attactgccc tctctttcta
    30301 ttttcagcta caactttcat aaaaagtttt tctaagtttt gttcttttga tacttctcct
    30361 tgataaccca ttactccatt agaaattatt cctatttcat ctacaacttg ttcaacttca
    30421 gacaagatat ggcttgataa aataactgta attccttgtt ttggaaaaga acgaatcaat
    30481 tctctaagtt cttcaatacc aatagggtca agaccatttg taggctcatc taagattaaa
    30541 agttttgggt gatttaataa tgcaatagca ataccaaggc gttgcttcat gcctagagag
    30601 aattgacctg aacgcttttt aaaggtatct cttaaatcta cagtctctaa aacttcttca
    30661 atgcgagagt caggaagacc aagtaaagta gtatggactt ttaaattttc tcgtgctgtt
    30721 aaattctcat atatagcagg agactcaata agagaaccaa tatttgataa atcctttcta
    30781 gtccatctat gtccctcaaa cattattttt cctgaagttg gatttaaaat cccagttatc
    30841 atttttaata aagtagattt cccagctcca tttggtccta ataatccata tatagaatta
    30901 gatttaattg ctagtgaaat gttatcaact gccatctgct ccttaaattt tttagaaact
    30961 ccttttacct gcaaaatata attactcata attttcatcc tttcttttgt caaacttatt
    31021 tacaagaatc attctaaatc ataaatataa ggattttata agaattaaaa aacaaaaaaa
    31081 gatgtgttaa aaacacatct ttttttatct taattctatt aattttccat tgtagtatat
    31141 ctctacttct tcaatccaaa tgctattatc ttcttcttgg atgacatttt taataatatt
    31201 atttatagta tatattatat tttgttcatt caaataatca tctttccaag aaactctact
    31261 ttttttatct ggttctatca agtttatttt aacaagatta tctttttcct taacttctac
    31321 ttccatgtct aaaccattaa aatactcatt tgataatgta tctaagatta atcgcagttt
    31381 atccttaagt gaactttctt catcaatctg taagtacttt actattttct catcctttga
    31441 aattatctct ataggtattt tagtagtttt tttagaatta atcattttac tagttgtttc
    31501 ttcttttgca tcttcatcct tctttacaac tgctgattct ttagaatcaa ctagctcaac
    31561 tgcactgatg ctataatcca tacatgccgt actcaataaa gtagtagcaa ttattaaaaa
    31621 acttactgct attttattca taagtatccc tctcctaatt ctctttcgat acttattata
    31681 ccatagtata gattatattt ttatactttt aaatcaattt ataaattatt atagtaaatt
    31741 atttacaatt ctaagctatt tgaatttatt tttatagtat attctttctc taaattcgac
    31801 agatttttat tcaaaagccc ttttatttct ctcgtatttc tatacttgtt gctatgttct
    31861 tcactatgtt cataatcata tgaatcattg tcatattctt catcattatt aaattcttca
    31921 attgcttctt ttgactttat atcacaatta aatttatact tatttactgg tatatcaagt
    31981 cttaccttag aatttggaac ttctatgtcc aggaacttag caattggcat atcagagtac
    32041 aagtaaaatt cactttcata atttgaattt ctacctattt tctcttttat atttacattt
    32101 tcaggtatat actcttctat attattgata aatacatctt cattctctga agatatatac
    32161 aatgactctg attctatgtt tacattttta acgcctaaaa ttgattttgt tgacaagtga
    32221 agattgttat tagatattaa gtttaggttt tctaatctat ttactttgtt ctctgttata
    32281 gctctactaa aacctccata agaagtttta tatgttatag tatttgaagc tacattatcg
    32341 tatacaaata agtgtgagct tatacttgat gcatttatgt ctacattatt tggtacatat
    32401 attataattt ctctataatc atgtgaaaac atactattta tagatgtatt taataattgg
    32461 tcgaaatttt taatcttttt aattttattt tcatattttc tttcaccttt tacaactaaa
    32521 gtcttatctt ttaaagttac attgtatttg taaaactcat tatttcctct tgttgttaca
    32581 cgcacatctt cacttgaact tttctttatt gtaacgttta tattgtcaac agctatatct
    32641 aacttgtcaa tatttacttt ttcttgataa agtttatttt cttttttaaa ttcagcttcc
    32701 ttttctaaac caaagtttat taggcttggc ataactaaaa tccctgacca tgttgtccct
    32761 attataccta ttaccaataa aaccgcagca aatatagcaa gttttttatt catgtttatc
    32821 ttctcctttc ttattttcta tatcttcaag gtccttatat atcgcctcaa aatttatact
    32881 atcatcatta tattcatcag tttcttttat gctttcttct tctctattgt agtaatcatt
    32941 attatatgaa tcttcattaa tgtctttatt caaatcttca tcatttaaag tagctttttt
    33001 tcttttttcc atcttttcat ttgcttttat ataaatattt tttctcttta tccaatgtat
    33061 ataattattt agtaattgtt tacctagttt tatggcaaat aataaaattt gccatgcaag
    33121 tatctcaagc cctacaaatg ctatggttgc gaaaatacaa aacatcataa catctggaac
    33181 cattgctata aatttaatca caaatggaat taatataaca aaagcaatta atgagcatac
    33241 taatgcaata gtagcagata caatagcaca tacaaacgca aatgctggaa caattagaaa
    33301 taaagataac actactaata acccctttat aacttgtctg gataatcctc ctacaccttc
    33361 ttctatactt ggtgtaagtt tatcatttat attttctttt gatttattgt acttttcctt
    33421 agcttcatct ttcaagatag aataatcctt ttttacattt gatttaaatc tactaaattt
    33481 accatttaat atgtctgtat tcattatttt tccatctata ttttcttttg atttactata
    33541 cttttctttt gcttcatctt ttaagataga gtaacctttt ttcatatctg atttaaattt
    33601 actaaactta tcattcaaaa catctgtatt tattattttt tcctcttcaa tatttttttc
    33661 tttagtttgt aagataagtt cttttgctat agattttggt gagccaagtg atgctattat
    33721 ctccatatca ctctttccct ctataagacc atctacaaaa tactcttcat aatctctcaa
    33781 tatatcatta acttcgctct cagaaaaatc tttttttaga taatccttta aaatttcaag
    33841 aaactcagtt ttattcaagt tcattacctc cctcatcttc acttattaaa atctcaacag
    33901 cctttacaaa ttctctccac tcttccatca gattggataa attctctttt cctagtgaag
    33961 ttattctata gtattttcta gcaggacctt cattagattc tagtatatat gtttcgaaat
    34021 acaaatcttt tgtaagtctt cttagaatag gatatattgt tccttcattt acatctatta
    34081 ctttagagat gttatgtact atctcatatc catacatatc tttcttagat atgagagcaa
    34141 gtacacaaat ttctaaaaca ccctttctaa attgtgtgtt catatactcc atcctttctt
    34201 taaatacaca gtattgtgtt ttacaaggta ctatgttaaa acttcttata tcctcataat
    34261 aacacagtac tatgttctgc acaatagtta tttttaaata ttcttgttga ttaaatcctt
    34321 ctataaatgt ataatactct aatcagatat attttatgaa agtggtgata aattggctaa
    34381 aatgaaagat gataaaacaa tattaatcct aactgctcaa tttggtgctg gacatataag
    34441 tgctgcaaaa gcagtcaaag aatgtattat tgaaaaatat agtaactata atgttgttat
    34501 acaaaacttt ataaatgcaa gtattcccat gatgaataag cctatggtaa aactttatga
    34561 aaacaataca aaatatactc ctggattata taattactat tattacttta agaaatcgtt
    34621 tgattcaaga catgatttat ctcataaatt atatacacct aaactttctg agtatattgc
    34681 tgatataaat ccagatttga ttatctctac atttccacta gctgctgctt gtgtaaataa
    34741 ctttaagata aaaaatcctg atataaatat acctacactt acagttataa cagatgttgt
    34801 agatagtatg gaatgggttt ttgaaaatac agatttgtat tttgttcctt ctcctgaaat
    34861 aaaaaataga ttttttcaga aaggaataaa tcctgactcc ataaaggtta ctggagttcc
    34921 tgttgataag agatttcaaa ttgaaagtaa agaactatgt tgtgataaat atagactatt
    34981 acttctaggt ggaggaagag gtctatttga tatagatgag gattttatgc attggataga
    35041 tgaatttatt gaagaacata gtaattctat agaaattaca atagttactg gtaaaaacaa
    35101 aaaattatat gacaatctaa ctcataaaaa acctctaaaa aatattaaag tacttggatt
    35161 tgtaaacgac atgtataatc taataagaga atgtgaccta atgctaacta agcctggagg
    35221 agcaactata tttgaagcta ttcaatcaca aactccagta ctcgtaaaaa tgccaaaagt
    35281 tggacaagaa attgaaaacg ctaaatttat aatagataag ggtcttggca tgatttatag
    35341 tgatgaccta gaccttaaaa acatatttta taaattagta tcaaatgaat ttgattctat
    35401 aataaacttt atgaaaaaaa atttaaaaga atttaaaaca gtcattcatc cagagaaaat
    35461 tgctgattat atatcagaat taatagataa tcattacagt taaattgtac tattaaaaaa
    35521 tttcaaacat aaaattaaag gcaatcaagt atgattttac acttgagaat ttactcatag
    35581 tgtttaataa tacttgattg cctttatcat atcaaaatca aattgttata ttagtatata
    35641 tttttcttgt actgcatata cagttgttgt atttcttcca tacttctctt taattctatt
    35701 tgtaaatctg aagcagtctg gtaatcctta ttttctatag cttctctcac tctagtgaat
    35761 agtgattctc tagttaaagt atccttcata atattgaact ttaaatccga tattgagttc
    35821 caaactacct catcaagcgc atccatattt tcttttgtgt gtatcttgac aatacttctt
    35881 attttttgaa tcccagcctc tattattcta gtttttaatt tcttttgcca cttatctata
    35941 gctccaatct taaatgattc tattatacta tcagtaaata catcaccttg ttttaaagat
    36001 tttaattttg aagcatatat ttctaagttt ttcatatttt cataaactgt agctggtggt
    36061 attccaaatc ttgcatttct ttcttccaaa ctataatgtt caaatacatc attttcatct
    36121 ctatatgctc tatctttttc taaatagaaa ctttcttcac ctacattttt agataactct
    36181 ttttcaagtt cttttgtact aagaccactc ttagctgctg cttttatacc atccaacatt
    36241 gtttgataac atcctgctat tactaaatac gtgttagata gagggcttgg tgaccttaat
    36301 tcaaatctta cagtctttgg atttttcata tctcttatta atccaacaag cactgaccta
    36361 tttcttgatg gaatctcata actatgccct aaagatgtaa ctatacatac aggagcttca
    36421 aatccaggaa ctaatctatt aaatccatca ttagaagctg ttacaattgg gtttaatact
    36481 tcataattgt aaagaagtcc cataagagcc ccatatccta gttcacttag ataatcttct
    36541 tttaaatctt ttggagcaaa tagatttttt atacttccat cctttaactt cgcacttact
    36601 cctacatgtg tatgtccacc acttcctgct actccatgaa ttggttttgc tttaaatgta
    36661 acttctagtc catgtgatgt aaatacatcc tctataacat ctcttactaa taattcattg
    36721 tctgctgctt gtaatggtgt agaaaacttc caagaaacct ctaattgctc catagcatgg
    36781 tttgttcttc catctatact aatagaactt tgtattcctc cgacctcttt gtgagccatt
    36841 tcagggttta cacctagctt ttgaagtata attaaacttt tctctaaaca agttcttata
    36901 ataccatgag ttcttttcca gtattgctct tttaggcttt gtgatacata taatttctct
    36961 aagtcagctt tatcctcagg agtattaacc cagaactcaa gttctgtagc tgctgttaac
    37021 attatttcct ctatatcatc tactgaatct attcctatgt tatttattac atgtggatat
    37081 tctctaaata tttcatacat tgacttttta aagtacttat ctgctttttg aagtactcct
    37141 ctagaacata cttttttatt atcatgtatt aaaaatgcag gtatctttaa tgttccaaca
    37201 ggtagaccta cttcttcgtc tatatattcc atattgtaat ctacatacca atgacaactt
    37261 ttatctggca ttaaatctac tttcgcatta tttaatgtag ctatattata aagctcaaca
    37321 cttgaaccat cagtctgtat agctgattcc aaaaatttat ctatatcctc caaaaataat
    37381 tctacaggaa tcttttcatc tgttgcattt cctcctaaat caacccccat taaggaaaca
    37441 aatcttatat ttttattgct ttttaggatt tctcttaatt cttcattagt gtgtttatct
    37501 ttctctatta cgtataatag actatccatt tttccactct cccataattt ttatgtatat
    37561 tttattttgt taaatacaag ataaatttca ttttccttac atttatgaat ttttcttgca
    37621 aattattcgt gttttattcg tgtttttatt tatattatta tatatcaagt ataaaaagtt
    37681 cgcaatattt tttattggaa aaaatattat acatacaata gtttaacatt tttacaaata
    37741 tggggtatac taaaataaaa gtatttttat ttgaaaattt aattttattt ataatataat
    37801 ctatagtaaa agaaataata aaaaaattta ggagataaac tttatggaat acataatttt
    37861 cgatttggaa tttaaccaag gatttgataa aaaactcaat aaaactgtat ccaatgaaaa
    37921 gtgtcctttt gaaattatac aaataggagc aattaaattg gattctaaat tcaatataat
    37981 agatacgttt aatagttatg taaaacctac aatttataaa gacatacatc cctttattag
    38041 tcgtatgact aatattaaaa attctgattt taatgattcg cctactttcc ctgaggtcta
    38101 caacaatttt ataaaattta tatcatcaca agaccctata ttatgtgttt ggggagctgg
    38161 agatttaaag gaattataca ggaatatcaa ctatcacaag ctaccatcaa attccttacc
    38221 taaatcttat attaatatac aacaacatgc atctaaatat ttcaataatc cagcaggaaa
    38281 aagcattggc ctacaaaatg ctatttctat attagaactt gatgaaaaaa tgtcttatca
    38341 caatgcttta aatgatgcat attatactgc taaagttttt attaagatat acaatccaag
    38401 tatagtccca gatatatatc tttatacaag tataaaacct aaaactataa gatattctaa
    38461 caaaaaaaga gttgattatg ataagttatt tgatgaattt aggaaaattt taaatcgtga
    38521 attaactaaa gacgagaaaa aaatcattaa cttagcttat aatatgggga aaacaaatca
    38581 atttacatta gaaaatgtta agcaaagaaa aaacaaatag agtttattta gataaaaaat
    38641 aggcatgtag attaaaaaac tagttaactt taaatttata tctacagccc aatttttatc
    38701 ataattaatt tttattttat ctctaattca actggacaat ggtctgaacc taatatttca
    38761 gtgtgtatat ccgcacttac taatctatct tctaattttt tggatgcaca aaaataatct
    38821 attctccacc ctgcattatt ttttcttgca ttaaatctat atgaccacca actataaaca
    38881 ccttctttat caggattaaa atatctatat gtgtctataa acccactgtc taataattta
    38941 gaaaacttct ctctttcttc tttagtaaaa cctgcatttt tcatattatt tttaggattt
    39001 tttaaatcta tttctttatg tgcaacgttc atatctccac acacaattac aggcttatga
    39061 ttatctagtt tggttaaata atctataaag tcgtcttccc atctagttct atattctagt
    39121 ctctttaatt cagattgaga atttggcgta tatactgtaa caaagtaaaa atcttcaaac
    39181 tctagagtta tcactctacc ctctttgtca tgttcttcta tatttattcc atacattact
    39241 tttaaaggct ctttttttgt aaatatagca gtacctgaat aaccctttct ctcagcataa
    39301 ttccaatatt gaaaatatcc tggtaaatct aactctattt gcccttcttg caatttagtt
    39361 tcttgtaaac aaaatatatc tgcatctact tctttgaaaa agtctaaaaa tcctttccca
    39421 acacaagctc ttattccatt aacattccat gatataaatt tcatacttat actcctcatc
    39481 taaacattga cacactcccc atagctaaag atgggggctt ttcaacaatt ttattgtaaa
    39541 ttcttaattc acttttattt taacaatgct atactataat ttcatctaaa attactattt
    39601 acttaatacc taaaaatata tgttttaatc aatttttaac ttttttgtta aatcttcagg
    39661 gctcattgaa gaaacataca tatttgttcc aagttcaaat cctccaaaat tatattttct
    39721 tgggattatg tgcatatgaa cattaaaata cgattttgtt tcaatctttg ttggatgagt
    39781 gtgaatacat aaattaaatg gcatatatcc attgatatta tataacttct taaacaactt
    39841 ttcaaatatt gtagaaagct cctgtatatt attatcattt aactcaccaa actctatttt
    39901 atctttaaat attattctta cttctccact atacttggtt gcatatggta taagtaccaa
    39961 aaaagcttct ccattataaa caactcttct atctaaactt atttcatttt ctattaaatc
    40021 atcatacaaa gagcttttat tgctgtcata atactccttt gaaaccaaca gttcattcgt
    40081 aatttcaggt ggaataaaag acatagacaa tatttgagca tgtggatgca tcaaagatgc
    40141 tcctgccttt ctcaagaagt ttttgaagat actaacatac tctactttat catctttcga
    40201 caaatctcta aatctatttc tatacataaa aaaaacatct tcaaattctt tttgactcat
    40261 gttataaaaa ctacgattat gtttatcgct ctctatcatt acttcatgaa caccatatac
    40321 atctcttgcc atatcatcaa ttataggaaa tttattgtag acagatttta caagccatcc
    40381 ttcttcactt tctatttcaa ataatgcatc agttgcatga gcttcatttc ctttgcaaaa
    40441 tgggcattct tcatcatatt cattagtttc ttcatcttca tttgggatat ctgctgtatc
    40501 taaaggtctt tttaacctat ctgtagcaaa tataacaaca tcattggtaa ttggatctat
    40561 tcttaattct ttcataatta acacctctta gttaatagcc tttaatataa ttatatgata
    40621 tttatcgtat ctgttaaata aatttataat attttttata attttgcata tttattatta
    40681 tatattttgt ctatagctga aagattataa ctaaaaaatc actatcagta tatcagaata
    40741 ttatcttact ataaattaat ttatgaatac ataatttagt ggcataaaca tgggtatttt
    40801 actataattt gttaaaagtt tattttaaag ctatattaat tttttttgat gatttttacc
    40861 cataagtatg ttattattat cttgtaaaga tgtcttgtat tcaaacatac aatttacata
    40921 atacaatata cgtatttaaa ttaaataggg ggtattaaaa tgaattattc aaacagagtt
    40981 agtgcaatgc aagcctctcc aatcagaaaa cttgttccat ttgcacaagc agcaaaagat
    41041 aaaggaatca aagtatatca cttaaacata ggacaaccag acataaaaac tcctaaagga
    41101 ttttttgatg ctgttaaaaa ctttgatagc gaagtcttag aatatgctac atcagaaggt
    41161 attccagaat tattagaagc acttcaaaat tactataaaa cttacgatat gaattttgaa
    41221 aaagatgaat tattagtaac taatggtggt agtgaagctt tacttttcac tatgatggca
    41281 gtttgtgacc caggagataa tctattagtt ccagaaccat tctatacaaa ctacaatgga
    41341 tttggtcaat ctgtaaacgt agaagttaat gctgttacaa caaaggcaga aaacggtttc
    41401 caccttccat ctaaagaaga aatattgtct aaagtagatg ataaaactaa ggctataatt
    41461 ctttctaacc caggaaatcc aactggtgca atttatacaa aagaagaact taatatccta
    41521 gctgaaatag ctaaagaaaa agatttatgg ataatagcag atgaagttta tagagaattt
    41581 gtttatgatg ggcttgagta tacaagctgt ggtaacttag agggtgtaca ggatagagtt
    41641 ataataatag acagtgtttc taagagatac agtgcttgtg gtgctagaat tggttctatc
    41701 gcttgcaaaa ataaaggtct tatcgctcaa atccttaaat tatgtcaagg aagactttgt
    41761 gttcctacat tagagcaaat aggagctgtt gagttataca aaactcctgt ttcttacttc
    41821 aaagaagtta atgaagagta caaaaaaaga agagatgtat tatacaatga attaatgaag
    41881 gttgaaggtg ttatatgtaa aaaaccaact ggtgctttct atatagttgc taaattacca
    41941 gttgaaaatg cagaagattt cacaatttgg atgttaaaag aatttaacaa agataatgaa
    42001 acagttatgg tttgccctgc tgaaggattc tatgctactc caggtttagg acgtgatgaa
    42061 ataagattag cttacatctt aaatgaaaaa gaccttcata gagctgctac attattaaaa
    42121 gaaggtttag agcaatatgt agctttacaa aaataatttt ttaaacttaa tttatataaa
    42181 tttaatgata taaaatgtcc ccttttcaga tattatttga aaaggggaca tttcttaatt
    42241 aatatattaa ttaagactta gacctgacta tttttcattt tctttatata gtgttggcca
    42301 gtaatcttta ttcactttaa ttaaagcatc taaaacttgt cttgccttag ttggagagat
    42361 tatagttcta tttaatgtta aagataaaat agctttttca taagattgtt caaagtatgc
    42421 atcaactgct aacttttcat aagctagttg accttctaac agtcccttat aaaatgttgg
    42481 tatattacct acatgtaaag gtctcggtcc attctttgta agaatcgcag ctacttctac
    42541 cattgcatca tcaggtaagt tgctaatagc cccattattt tctgttataa ctatggctat
    42601 ttggtgtttg ttatgagcta ttgcttctgc aacttcaact ataaattccc catgagcatc
    42661 attatgcact aaattagaac ctatagatgt tcctttttct ataacagatt tacattcttc
    42721 aaatacacgc ttttctcttc cattcataac ttctcttgct ctagtacaat ttatatttga
    42781 tttagctact tttttatcag gatatagata atactgtaca tatgtattag gtaagtaatc
    42841 tggtacatct tccaacatat ctttaaccat tccataagtg tctaaccaag atttatctct
    42901 ttgttctgca tcaacaggtt ggaatccaga gtttgctact aaatctttta tcttaggtac
    42961 caaatcttca ccatttttat catacaaatg tgtaaaccat ccaaagtgat ttaatccaaa
    43021 gtatacaggc tcaaatgttc ttgaatccat gtctaacaat cttccataag accttaacaa
    43081 gttaactggt tggtcacaca tattaagtaa tcttttatca tctggaaata ctctttttaa
    43141 tgcttctgca acaatagcag caggatttgt ataatttaat atccatgctt taggagagta
    43201 ttttcttaca gtcttaacca tttctatcat atcacctata gaccttaacc cataagcaaa
    43261 tccaccagct ccacaagttt cttgaccaac cagtccaaat tgtagaggaa tcttttcatc
    43321 taattctcgc atcttaagac caccagttct catttgaaca aatacaaaat ctacatctat
    43381 aaatgcttca tctgggtttg tagtatattc aagtgttgct ttaggatatt cttctttaaa
    43441 taatattttt ccaaattccc ctattaatct ttgacgttct tctactacat ctaacatagt
    43501 tactttattt agaggaaatc tatcactcat tttacataaa gactttaaaa gtcctggagt
    43561 ccaagtactg cctcctccaa ctatagctat attgtatctt tttttcattg tcatattaat
    43621 cacccctgtt ttttattaac taacttgtaa gatgattata atatataaag ttttctacat
    43681 gaatactttt aggtaatcat taaaaatagt tcaattttag tatttttttc aaaattgtaa
    43741 tttttttcat ctattgtatt tttcaatgta tttatgaaac atatattcca taagagaaag
    43801 tgctggcact ctagatgtat agttaacact attaaaatta tattgtggag aagatacaaa
    43861 taattttata tcagctatat ttgataatga attgacactt atatttgtca gagaaattac
    43921 tttaacttga tttgtttttg ctatattaca tgccttaata acttgagcag tctctccaga
    43981 catagataca gcaattacaa ggtcatcttt agtaatattt gatgcagcca aatacatgag
    44041 agttgaatca tcatagtatt ttacatttac acctatagct gaaaaacgtt gagacatttc
    44101 agtacatact aatctagaag acccaacacc aaaaaaatat atatgcttag aattatgtat
    44161 ttcatttaaa cacatatcta tattctctac atttattaaa ttttgtgtat ccataacatc
    44221 agtaaaaata tcactttgac ttttagtaaa ctccttattt ctttcatttc tatttgaata
    44281 tgaaaaaact aaaaagtttt taaattcact atatccagaa aagccgagct tctgacaaaa
    44341 ccttactatg tatgcagtcg aattaaaagt ttcatttgct atatctttaa tctttttttg
    44401 atttattttt tcagaatttt ttagaaaata gtttaaaaga gatatttcat ttgtagataa
    44461 ttttttttga tttatatcta atttgtctat taggttcatt tatacctcca taataatgat
    44521 ttgtagttaa acaactttct catatttatt atataaatat acactataca gcatttatct
    44581 tatgtttatt atactataaa aaagacaata caaattataa attagtattg ccttttaata
    44641 tttactttaa atagacattg caaaagaaac ttcgtctctt acttgagata cagatggccc
    44701 ataaattatt tggatatctt tatcagatac ttttataact cccattgcac ctgtatattt
    44761 tttaaaagca ttttcatcta ctttactcat atctttaact actactctta agcgagttat
    44821 acagtttgtt atatcttcta tattgtcctt accacctaaa tatttaatta tagataatgc
    44881 tctcttagta gtttctccac tttgagctat ttctacttta tttgataaag gttttaaatc
    44941 aaaatttata tctgttattt cttctcgtcc tggagtttgt aagttaaatc taagtatcaa
    45001 aatcttaaat acattataat aaactattgc atacactaaa ccaagtatta tagaataaat
    45061 ccactttgta tttggttgca taactccaaa tactactaaa tctattatgc caccttgagt
    45121 atttcctatt gctactccaa atatatgcat catcataaat gatatacctg ataaaaatgc
    45181 atgtataaca aataatattg gtgatacaaa taagaatgaa aattccaatg gttccgtaat
    45241 tcctgttaca aatgttgcta aagcaccagc tagtaatata cctttaacct tcgacctatt
    45301 ttcaggcttt gcagttttat acatggcata tgcagcagca ggaagaccaa ataccattat
    45361 aggtattttc ccttgagcta aaaatctagt tgcttctgtt gaaaaactga ttgtttcagg
    45421 gtcacttagt tgagctaaga atatgttcaa tgctccaaat acttcttgcc ctcccacaac
    45481 taaagaacct cctacttcag taaatctaaa catagcattt aatatatgat gtaatccaaa
    45541 tggtattaaa agtctttcaa gaaatccaaa tacaaatgtt cctaaatatc ctaaactagc
    45601 tataccaata cctacactct gaataattgt atttacatat ggccatatat atggtattat
    45661 tatccccaaa atagagaata ctactgttgt tattattgga acaaacctag ttccaccaaa
    45721 gaatcctaaa taatctggca gttttatatt atagtattta ttatgtaatg atgctgttat
    45781 tataccaact acaattccac caaatactcc taattctaat gtttgtatgc ctaaagttac
    45841 agcttgaccc atttctctca tcatgtcagg ttcagcaata atccccctac accctaacat
    45901 ataatttaca ctagtgtgta atactaaata accaacaaaa ccagaaaatg cagaaactgc
    45961 tttttcttct ttagcaagac caacagatat tgatatagca aagaatattg ctaagtttcc
    46021 aaaaagtact cctgttatag cttttgtaaa acccaatatt ccttgaataa aattatttgc
    46081 taaaaaaggc atatcactaa ctatattagg atttgaaaat gctgaactta ttcctaaaaa
    46141 tatacctgct gcagatatta atgctattgg caataaaaat gattttccta actgtaaaaa
    46201 gaaccctttc attttttgtt tcatttctat ccctccattt ttaaattttt aaaacgtttt
    46261 ctatgtcata attatatatt tttattttgt ttttgaaact ttataatttg ttttaaaata
    46321 tttttacata aatagtaatt ttttcaaaac tgtaaatttt ttcaaacaaa gacatagtaa
    46381 cgttgtaaca tatataaatt taaaaagagc ttagaaaata aaattattga atataaaatg
    46441 gaaaaatata aattacacaa agagatatga gtacgtacta acaaatacga aaatgtataa
    46501 gaaattataa aaaagtacat caagaaaatt taagatgtac tttttagatt gtatttagac
    46561 ttataattat atagttaaat gaataacaaa gaattaatct tagtactatt aaatacaaaa
    46621 atttttatat tttttctatc taaattcttc tttgtttaat atatattgaa gagtatttat
    46681 tgcttctaaa tatttttttc tgtcttcttc agttaattta gaaagttttt ctcctaaacc
    46741 atcacttaat ttatccataa aatcagttgc tatcttatct ccctcttcag taatgtcaat
    46801 tagatatttt cttctatcaa ctaaatctct tttccttgtt acatacttct tatcagttaa
    46861 atcatcaacc atactagtta aactgccttt ttctatattt aacttagagc ataaatcagt
    46921 catacttatt actccataat tttttatgaa aactaatgct cttaattgtg ttttatttac
    46981 attataatct ggtgcaaatt tttttaggtc ttctaagtac aggctagaat aaatcttagg
    47041 aaatgttttt actaaaaaac ttatggtatc ttttactata tagttcatat aatcacctcg
    47101 gttgttttaa aatttattaa attatctaaa taactttatt tcagtttaca ttaaaaatat
    47161 tgcaagtaca atagtttttg ctgaatgttt aaatttaatt agtaatctta tcattttttt
    47221 atttttattt atattattcc tattaaatat tatttatata gctattttat taaattcatg
    47281 gtatagtaag taaagaagaa ctcttcacag cggtggagag aagacgctag cccattaaaa
    47341 tggctatctt atttataaat aagatagccg tttttaatta tattttatct gtacaattct
    47401 atactcttaa gtctacatct acttttccta agtcttcctt ataatctttt atagcaggtt
    47461 ctacaacttc atttacaaat tctacaactt gttctggagc tctacctata aagttttttg
    47521 ggtccattat agatagtatt tcttccttgc tcattttaaa tgaatcatca ttcattataa
    47581 gttcaattaa gttattatcc ttgccttcat gctttactct ataggcagct ttcatagaat
    47641 attctcttat tatttcatgt aattcctgtc tatctccacc tctttttaca gcttccataa
    47701 gtattgtttc tgtagccata aaaggaagtt cttcatttac acgtctattt atcatatttt
    47761 catatactac taaaccatca gttacattta tccctatttc aagtatagca tctgctgcca
    47821 taaatgcttg aggtattgcc aatctcttat tggctgagtc atctaatgtt ctctctaacc
    47881 attgagtggc ttgtaccatt gcagggctta tagattcaga gattatatac ttagataaag
    47941 atgctattct ctcacttctc attggattcc tcttataagc cattgctgat gaacctattt
    48001 gatttttttc aaatggctct tctaattctt ttaaactttg taaaagtctt atatcattag
    48061 tcatcttatg catactttga gctatccctg atagtatgct tattacttga taatctaact
    48121 ttctagtata tgtctgtcca cttactgcat aggaagaatg gtatcccatc tttttacaaa
    48181 ttttcttatc taactctttt actttttcgt ggtctccttc aaataagctt aaaaaacttg
    48241 cttgagtacc agtagtacct ttaactcctc ttaacttcat gttgtctatt ctatagttta
    48301 aatcttccaa atcaagaatt aaatcttgag cccataaaca agctcttttt cctactgttg
    48361 taagttgagc agcttggaag tgtgtaaatc caagagctgg catatctttg tacttaagtg
    48421 caaattcttt taaattgttt attaaattta caagctttac tcttagaagt tttaaagctt
    48481 cattcatttg tataacatct gtattatctc caacatatgc agatgttgct cctaaatgta
    48541 ttattccttt ggctttttca cattgtaaac cataagcttt tacgtgagac attacatcat
    48601 gtcttacttc tttttctatt tttctagctt catcaaaatt tatattatct atattagctc
    48661 ttagctcatc taattgttct tcagttatat tgattcctaa ctcatgttca ccttctgcaa
    48721 gtgcaaccca taaccttctc catgtagaga atttaaattg tggagaaaat atataactca
    48781 tgtccttgct acaatatctg tctgtaagtg ggtttgagta agtagtatat ttattatcta
    48841 tcattatatt agcaccttcc ttaaatcttt tgtcgtgtga ttactgtgta cacattagta
    48901 cataaataat tatatataat tttacgaact ataacaagac ttttttattt tttgttcaca
    48961 tttatatata gtatttttta tttattacta ttttttattt atatccaaat tatctttttc
    49021 aattaagtaa tccactattt cactatcaac aaaatttaat acatacttat atctaattgc
    49081 atagtattta tcactccatc caagctgatt ttttacatca ctttcatctg ccccaagcct
    49141 taaacttatt gctgcaaatg aatgtctaaa atctcttgct gaatattgag aaagtccagc
    49201 taaatctaat gcttttttga ctataagtct gacatttcta tctgttatag aattactctt
    49261 ttgagttgta aaaataaaat catcactagg tattattacc tctggaagcc cagaataatt
    49321 cctgtaatct tctaaaagtt taaaaaagta tggatgtaat ttaactactc tttcttttct
    49381 tcccttacct aaccttacat atgagttatc gttttcatct accataaggt ctttccactt
    49441 taagctaact aattcattta ataaacaccc agtagtaact agacagacta ttattactct
    49501 atctcttata tttaattttg gtaggatacc tataagttta tttatttctt gtatacttag
    49561 aacatcatct tttgttttta ttcttgttac tgtgggtttt tctacatatc taaatggatt
    49621 tatatctatg tatccttctt tttttaagaa gttataaaaa ctatgtaaat aactatatat
    49681 cttttcacaa gttgatttcg catatttagt ctgtatatag tctacaaaat ttttacaatc
    49741 ttctttcgta gcataaatca attctttccc ttttaaaaat tctttaaaca gtataacctt
    49801 tgataaataa tcgtgtttcg tgtttggatt taattttgta gaaaaaacat taaaagtttc
    49861 atactcataa tcatctagta tttttgtact atacataata actcctttcc ttttcataaa
    49921 ataaaatagc tatactttta ctataccata tatttccccc tcaaatatac cccttattca
    49981 atagataact tttaagttta ttttgttata ttgtgaaggt tttatgaaaa tatcaatctt
    50041 ccattgtgca ctacacagta ctatggtatt ataagttagt aaaaggtggc atttttttat
    50101 taatctatga ataaaatatg ctactataaa tttgtattgg aggtattttg atttggaaac
    50161 atttagtttg cttgaaattt ttaaggcagt cattttgggc atagttcagg gaataacaga
    50221 atggctacca gtaagtagta cagggcatat gatattagtt gatgagttta taaaattgaa
    50281 tttttcaaat acatttataa gtacattttt agttgtaatt caatttggtt caatattagc
    50341 tgtacttgtt atatttttta gaaaattaaa tccttttgat agcgcaaaga atattaagca
    50401 gaaaaaagaa acagtccgtt tatggcttaa ggttataatt gcagttatac catctggagt
    50461 tattggaata ctgtttgaag atgatataga tagattattc tttaattcaa cagtagttgc
    50521 tattgctctt attgtatatg gtataataat gatagggctt gaaaagagaa ataaaaaacc
    50581 taagtataaa gatttttctc aagttacata taaattagct ctttgtatag gattattcca
    50641 gtgtctagct cttataccag gtacatctag gtctggttct actataatag gtgcagtatt
    50701 acttggtact tctagatatg tagcagcaga gttttcattc ttcttagcca tccctacaat
    50761 gcttggagct agtgcattaa aacttctaaa agctggattt ggatttactg gatttgaatg
    50821 gcttatttta ggtgttggtt ctgtagtagc atttgttgtt tcaattgtag ttattaaatt
    50881 ttttatggac tatatcaaaa aacatgactt caaagtattt ggttattata gaatagtctt
    50941 gggtatagtg gttcttgctt atttctttct attataatgt attctaattt aataacaaat
    51001 gataaggaga aaagttatga gtgttaaaaa agaaatattt gattggatta agtcaatagc
    51061 tatggctatt gtacttgcat ttgtaattct acaatttata agaccttcta ttgtaagtgg
    51121 agaatcaatg tatcctactt tagatgataa agattatctg attttaaata ggatatcata
    51181 caaggttggt aaacctgaaa aaggcgatat tgtagttttt aaaaccaatt tagttgatgg
    51241 agaaacagga aagaaaaaag acttaataaa aagagttata gctactgaag gtgacagaat
    51301 aaaaatatca aattctaaag tgtatgtaaa tggaaaatta ttaaatgaac catatataca
    51361 caataactat acttctggag atatagatac tgttgttcca aaaggtaaac tatttgcaat
    51421 gggagataat agagaaaata gtaatgatag tagattccct gatgtaggta tggttgatga
    51481 agatgaagtt cttggtaagg ttatggtgag actattacct cttgataata ttgggaaagt
    51541 agactaaaaa ataagagact gaattggtaa agtttatttt acaaatctca gtctcttttt
    51601 attttcttaa attacaaata tttttttact aaatatcttt tgattctgta ataagtctta
    51661 ttatatcact tgcttcttca ttaaaagttg ttactatact taggtagtct gttgctctac
    51721 tcatagctaa ataaacctta tttaaatctc ctacaaagtc atttacttga taatcttgtt
    51781 caatatcatt tatagtttga ttgtataaca tctctaattc acatactatt accgctttat
    51841 actctaaatt ctttattgca tatatatttg ctattgtagt tcctaccttc ttacttatat
    51901 ttgtcaaatt atcttctgca catatataag gaatttttgc ctcttccaat gcttttttta
    51961 acatatattg aaagtaaatg gtttttccac tttttaattt cttcttattg taaggataaa
    52021 cgatagcaac ttcagaataa tcaagacctt ttttacttat taaatactca atttcccaga
    52081 ttacagaact tatttggtca tcaaggtcac ttacttttat tatatcaaca gacttatttc
    52141 cacctctaag tgctttagta taaaagaaag tactaaattc tgtatttggt cttaattcgt
    52201 tgatatatga gttagaatta tttgaaaatt tatttgtaaa atctacaatc tctttagctt
    52261 gtctataatt tttacttaag ataatcttat catcaaattc tataccatta tataattttt
    52321 taaatatatt caatctatta gagatattca aggagttaca agaacacaca ttaaatatga
    52381 attttgtttt atataaaaac tcccttataa aatctatttc atactctaaa aagctttctg
    52441 cttcatcaat aaaaatagcc ttgaacatat ttttattttt tataatgttc tgtgcttgtt
    52501 ttacaagatt attaaaggtc ttttcgtaat cattttttaa catgttataa tctacaatta
    52561 aattgaattt tttagctagt ttaaatataa aagaactaaa tgtgtgaact tctaaattgt
    52621 tattatcttt gtataataac tctatcctct ctcgaagctc attgcacaat tgttttgtat
    52681 gtgtaaatat gactaattta tgatgtggat atacttttgc taattttata gccctagaaa
    52741 gcataattgc tgttttgcct ataccagaac caccttcaat aagtgtattc ccatatttaa
    52801 tagaaacaac atctttaatt tgaacgtctt ccatcatagt tgctgtatac ttatagtcat
    52861 cattgtagaa tgaaattttt ttaaattttt catttagatg aagtttatca tttattatat
    52921 agtattctga acatatgtct agtaaaaata aatttaagtt tatttcatca ttctcatcat
    52981 taagatattc ctccagcgaa cccttattca ttatatcttg aagcttattt ttatctatta
    53041 tattattatt aacaaattct tcaaattcat atgtttcctc aacttcaacg tatggcataa
    53101 tgtaaacgta gttataactt atatttctat tcttttgtcc catttttagt ttcaaaagtt
    53161 catattcctc attcataact tcaagtaact cttcttctaa aataaaaaat atatcttctg
    53221 ttgtatccat aaattttata aataaaattc ttccatcttt aacatacaac aaatcggtat
    53281 ttattccttt aaaaggagta acttttggca ctactttaac gtcctcattg atattttcgt
    53341 aaaatatttt ctcgaactcc ttcaatttta gggcgtctaa ctgagaattt atatttatgt
    53401 attggctcac ttacattacc tccttctgtt atacttaatt ataccatttt gaactatatt
    53461 tttatatata ctttttatag gcaaatttag taaataaatt caaaataaat acaaataaat
    53521 acaaaactgc taaaaaaatt ttgcaataag tata
//
LOCUS       DAMKMI010000009        51761 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMKMI010000009 DAMKMI010000000
VERSION     DAMKMI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.009"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            156..539
                     /locus_tag="PC429_000525"
     CDS             156..539
                     /locus_tag="PC429_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF3846049.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            541..891
                     /locus_tag="PC429_000526"
     CDS             541..891
                     /locus_tag="PC429_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846050.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(992..1450)
                     /locus_tag="PC429_000527"
     CDS             complement(992..1450)
                     /locus_tag="PC429_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846051.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1495..2283)
                     /locus_tag="PC429_000528"
     CDS             complement(1495..2283)
                     /locus_tag="PC429_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3846052.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2422..2859)
                     /locus_tag="PC429_000529"
     CDS             complement(2422..2859)
                     /locus_tag="PC429_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF3846053.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3681..4190
                     /locus_tag="PC429_000530"
     CDS             3681..4190
                     /locus_tag="PC429_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3846054.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4604..5035
                     /locus_tag="PC429_000531"
                     /pseudo
     CDS             4604..5035
                     /locus_tag="PC429_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5222..5415)
                     /locus_tag="PC429_000532"
                     /pseudo
     CDS             complement(5222..5415)
                     /locus_tag="PC429_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            5517..6023
                     /locus_tag="PC429_000533"
     CDS             5517..6023
                     /locus_tag="PC429_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF3846055.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            6028..6304
                     /locus_tag="PC429_000534"
                     /pseudo
     CDS             6028..6304
                     /locus_tag="PC429_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
     gene            6573..7145
                     /locus_tag="PC429_000535"
                     /pseudo
     CDS             6573..7145
                     /locus_tag="PC429_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7248..8063
                     /locus_tag="PC429_000536"
     CDS             7248..8063
                     /locus_tag="PC429_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF3846056.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            8144..8620
                     /locus_tag="PC429_000537"
     CDS             8144..8620
                     /locus_tag="PC429_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF3846057.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTNFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            8634..9197
                     /locus_tag="PC429_000538"
     CDS             8634..9197
                     /locus_tag="PC429_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3846058.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEVLKNVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITRWCYG"
     gene            9452..10363
                     /locus_tag="PC429_000539"
     CDS             9452..10363
                     /locus_tag="PC429_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3846059.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKR
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     SEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            10441..10875
                     /locus_tag="PC429_000540"
     CDS             10441..10875
                     /locus_tag="PC429_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF3846060.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            11343..12083
                     /locus_tag="PC429_000541"
                     /pseudo
     CDS             11343..12083
                     /locus_tag="PC429_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            12034..12645
                     /locus_tag="PC429_000542"
                     /pseudo
     CDS             12034..12645
                     /locus_tag="PC429_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            12858..13715
                     /locus_tag="PC429_000543"
     CDS             12858..13715
                     /locus_tag="PC429_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3846061.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVTSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            13793..14374
                     /locus_tag="PC429_000544"
     CDS             13793..14374
                     /locus_tag="PC429_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3846062.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            14478..15323
                     /locus_tag="PC429_000545"
     CDS             14478..15323
                     /locus_tag="PC429_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846063.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            15320..16003
                     /locus_tag="PC429_000546"
     CDS             15320..16003
                     /locus_tag="PC429_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846064.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIICFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            16000..16698
                     /locus_tag="PC429_000547"
     CDS             16000..16698
                     /locus_tag="PC429_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846065.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            16710..17621
                     /locus_tag="PC429_000548"
     CDS             16710..17621
                     /locus_tag="PC429_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF3846066.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALNIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            17673..17813
                     /locus_tag="PC429_000549"
     CDS             17673..17813
                     /locus_tag="PC429_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846067.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            18104..18730
                     /locus_tag="PC429_000550"
     CDS             18104..18730
                     /locus_tag="PC429_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3846068.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            18852..19568
                     /locus_tag="PC429_000551"
     CDS             18852..19568
                     /locus_tag="PC429_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3846069.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            19718..20314
                     /locus_tag="PC429_000552"
     CDS             19718..20314
                     /locus_tag="PC429_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDF3846070.1"
                     /translation="MKILWIIIIMADNLLPLLLARFYPNYKHKEMALSVLGSRQSPVK
                     WIYNVWCIISGVVFCIAPYALYQENDSGWAIAIWMLLAIYGIGCEIISGVCPLNENRQ
                     DEDIITKIHGGTSAIGFMALLVVPLLFAILQFQTTKVVLGAISLVCFTIAFIFFCFFI
                     MGEKEKFANTVLRYGGLWQRLVLISCYIPLFVWSVSNQ"
     gene            20401..20592
                     /locus_tag="PC429_000553"
     CDS             20401..20592
                     /locus_tag="PC429_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF3846071.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            20616..20846
                     /locus_tag="PC429_000554"
     CDS             20616..20846
                     /locus_tag="PC429_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846072.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            21113..21532
                     /locus_tag="PC429_000555"
     CDS             21113..21532
                     /locus_tag="PC429_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3846073.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            21535..21789
                     /locus_tag="PC429_000556"
     CDS             21535..21789
                     /locus_tag="PC429_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3846074.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            22164..22367
                     /locus_tag="PC429_000557"
     CDS             22164..22367
                     /locus_tag="PC429_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF3846075.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            22445..23635
                     /locus_tag="PC429_000558"
     CDS             22445..23635
                     /locus_tag="PC429_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF3846076.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKEKQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(23892..24692)
                     /locus_tag="PC429_000559"
     CDS             complement(23892..24692)
                     /locus_tag="PC429_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846077.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(24887..26485)
                     /locus_tag="PC429_000560"
     CDS             complement(24887..26485)
                     /locus_tag="PC429_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3846078.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            26813..26989
                     /locus_tag="PC429_000561"
     CDS             26813..26989
                     /locus_tag="PC429_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846079.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            27163..28293
                     /locus_tag="PC429_000562"
     CDS             27163..28293
                     /locus_tag="PC429_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF3846080.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            28534..28884
                     /locus_tag="PC429_000563"
     CDS             28534..28884
                     /locus_tag="PC429_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF3846081.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            28892..29341
                     /locus_tag="PC429_000564"
     CDS             28892..29341
                     /locus_tag="PC429_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF3846082.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            29443..30018
                     /locus_tag="PC429_000565"
     CDS             29443..30018
                     /locus_tag="PC429_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF3846083.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            30011..31420
                     /locus_tag="PC429_000566"
     CDS             30011..31420
                     /locus_tag="PC429_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF3846084.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            31604..33037
                     /gene="eutA"
                     /locus_tag="PC429_000567"
     CDS             31604..33037
                     /gene="eutA"
                     /locus_tag="PC429_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF3846085.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            33055..34419
                     /locus_tag="PC429_000568"
     CDS             33055..34419
                     /locus_tag="PC429_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF3846086.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            34432..35313
                     /gene="eutC"
                     /locus_tag="PC429_000569"
     CDS             34432..35313
                     /gene="eutC"
                     /locus_tag="PC429_000569"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF3846087.1"
                     /translation="MNEKDLKALVEQLVGQMLGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            35333..35986
                     /gene="eutL"
                     /locus_tag="PC429_000570"
     CDS             35333..35986
                     /gene="eutL"
                     /locus_tag="PC429_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF3846088.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            35997..36698
                     /locus_tag="PC429_000571"
     CDS             35997..36698
                     /locus_tag="PC429_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF3846089.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            36701..38170
                     /locus_tag="PC429_000572"
     CDS             36701..38170
                     /locus_tag="PC429_000572"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF3846090.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            38266..38553
                     /locus_tag="PC429_000573"
     CDS             38266..38553
                     /locus_tag="PC429_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF3846091.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGATAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            38680..39441
                     /locus_tag="PC429_000574"
     CDS             38680..39441
                     /locus_tag="PC429_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF3846092.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            39454..40083
                     /locus_tag="PC429_000575"
     CDS             39454..40083
                     /locus_tag="PC429_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF3846093.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            40125..40850
                     /locus_tag="PC429_000576"
     CDS             40125..40850
                     /locus_tag="PC429_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF3846094.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDGFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMNGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDILNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            40863..41135
                     /locus_tag="PC429_000577"
     CDS             40863..41135
                     /locus_tag="PC429_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF3846095.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            41128..41679
                     /locus_tag="PC429_000578"
     CDS             41128..41679
                     /locus_tag="PC429_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF3846096.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            41691..42782
                     /gene="eutH"
                     /locus_tag="PC429_000579"
     CDS             41691..42782
                     /gene="eutH"
                     /locus_tag="PC429_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF3846097.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            42784..43257
                     /locus_tag="PC429_000580"
     CDS             42784..43257
                     /locus_tag="PC429_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF3846098.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            43770..44291
                     /locus_tag="PC429_000581"
     CDS             43770..44291
                     /locus_tag="PC429_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3846099.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            44670..45845
                     /locus_tag="PC429_000582"
     CDS             44670..45845
                     /locus_tag="PC429_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3846100.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDILKLGCEFMAKYAVEFLNKNNN"
     gene            45879..47417
                     /locus_tag="PC429_000583"
     CDS             45879..47417
                     /locus_tag="PC429_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF3846101.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            47449..48651
                     /locus_tag="PC429_000584"
     CDS             47449..48651
                     /locus_tag="PC429_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3846102.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(48818..49417)
                     /locus_tag="PC429_000585"
     CDS             complement(48818..49417)
                     /locus_tag="PC429_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3846103.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            49587..50945
                     /locus_tag="PC429_000586"
     CDS             49587..50945
                     /locus_tag="PC429_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846104.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            50938..51570
                     /locus_tag="PC429_000587"
     CDS             50938..51570
                     /locus_tag="PC429_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF3846105.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
BASE COUNT        19339 a         6956 c         9309 g        16157 t
ORIGIN      
        1 atatagaaaa tccttataaa aatttaaatt gtaatgaata ggaagtaatt aaaaagaatt
       61 tttattgatt tttaaaaatt tagttagaaa ctatatcatt atggtaaaaa atgtattata
      121 atgtttagaa atattataat aaggagcgat taaacatgaa tctatcgaag aaaatagaag
      181 ataaaagaaa agctaaacag agagctgaaa aagttaagaa agctaaaatt gctacagctg
      241 gtgtggtatt aggagctgtt actggtgcag tgagtggagt acttttggct cctaaatctg
      301 gaaaagaaac aagagaggat ataaaagatg cttcacaaca aattgctgaa aagataaata
      361 tgaaaactgt agatgtaaaa ggcaaagtat ctgagaagct agaggataaa aaaggaaact
      421 ttatagaatc aaagaaaaaa ataaaaaaat atatagataa caaaaagagt gttgaaaatt
      481 ctagtgagga agataaaata atagatatag ttgaaccagt tagtgttact gaggaataat
      541 atggacaata caataacgat taacataagt ataatatatg tattattagc tatacttgct
      601 tgtattgctt tagtttattt aattattgta cttgttaaac tacataagat tttaaataat
      661 gttggaacta tgttaaatga aaatactcaa aatataaata caactttaaa taatcttcct
      721 aatgttgtag aaacttttgg agaagtagga gaaaatatga aagatgtaac agaagtagtt
      781 actgaagtta cagcagattt tcttgttact aaagacagtg tcaaatcaaa tatggatgtt
      841 atagttgata tattgactat aattaaaaac atttttgtag ataaaaaata ataagttaat
      901 ataaaataag cctataagat ataatttaaa cgaccttaaa atagcattta taaggtcgtt
      961 ttttattagc ttgatttgac actatgtgtt tttaataatt ggtaatattc ctctttatca
     1021 tttgaacata tatatctatg cttgttgttt caattacatc cataagaaca tctttacatt
     1081 caagctcaaa atcttccatt ccttctaaat attcatcatc ttcatcaaaa aagatataag
     1141 aattatatag ccattcttca aatttttctt caagtatatc ccaatacgaa ctccaaaata
     1201 atccacaacc tgatatataa gatgctctac tttgatttat ataaacttca tttagaagct
     1261 tttgtaatct actgctataa agaaaatctg ctcttccact gcttgatatt aattcattta
     1321 aatcttctat tgacattaat tcaactttac cacttttatc ataaggagta taattttcat
     1381 caacccattt atgtggtttg ttttctctgc atactgttgc tatcaactct acaacttttg
     1441 ttcttatcat ttttttatcc taacttattt attttttata aaataatcat gattttataa
     1501 tttatctatt tcttgtaatg ctaatttaac agtttcatat ctatcagcac catttattac
     1561 tacatcacct tttacaagtt tattagcttc tccacctaca acataaacct ttttacctaa
     1621 accttgctta taactagacg cttcaattat taaagaatct ttcaattgcc aatgtaaaat
     1681 ctgagcagcc actttatcag catcacctgt ataaacgatt acattttcat acactttatt
     1741 ttcccctccc ccttgagaat ttgatataga tttgtttagt ataccttctg ctatcaattt
     1801 agcaacttta tctttattat taacataata atctgtatct gctttactat ctacaaaaca
     1861 tacttctatt aatattgctg gtgcaatagt atgattaagc caataaagac ctctaacatc
     1921 agactttgca cccctatctt tgaatactgt agctaaccta gtattcactc tttctgcata
     1981 ggttttgcca ttattagtct tatatattgt ttctgtccct actggagttg aagttgtagc
     2041 atttgaatta aaatgaatct gaacagccaa atcagtttcc tgtttatttg ctatagcaca
     2101 ttggtctgct aaatgattac ttgattcatc aactcttcct gtataaacag tagctccacc
     2161 tattttaagc catttaacaa ttaagtcatt cagtattctt gtttctttgc tttcgtttat
     2221 atacccaaca gctcctgtac cttttccaat taaagtgtgc cctggtatta ttactacttt
     2281 caaactaaca tctcctttac aattaaattt aacttctatt taatatatgc ataacctaga
     2341 attttgtttc tttttatatc ttatactttt attataaccc attttgtaaa aatatagaat
     2401 attaagtaaa taatgttaaa attataattt tgtatataag acattacttg attgcaataa
     2461 ttttatagca tattcatcta ttctatatcc agttctataa tatatatgtt taattccagc
     2521 ttgaattata gccttagtac aatttaagca tggaaagtga gttacataaa tagagcaatt
     2581 tttaactgaa ataccttctt ttgcacaata ataaagggca ttttgttcag cgtgaatggt
     2641 tctaatacaa tgtccatctc tcatttcatg accaacttca ctacaatgct tatctccaga
     2701 aatactacca ttatatccag tagacactat tctgttttct gaattgacaa ttatagctcc
     2761 tacataagct ctatcacaag ttcctctttc agctactgtt tcgcacagtc tcataaaata
     2821 ttcttgccat gagcatctat tttcaagttt tttattcaaa tcatatacct ccaaaattta
     2881 tatctaatat ataagattta taccttaaaa ataatattaa aaacaaattt atatgataaa
     2941 taaaatttat taatactgtt tttagtctaa attatttcat tttaaattaa agttacaagg
     3001 aatctaagat tatatatatc tttcttcgta atatgaatgt tacaatataa aaaactgtct
     3061 caataaaata gagacagttt tacagtcaaa gtttataata caagttcata tttttcttta
     3121 aattctttta cagcttcggg ccatgccttt tgttctgtaa tacctttttc tttagctatt
     3181 ttaattgcaa tctctcttat cattgcagcg ccttctaata ttcccatttt taatctccct
     3241 tcaaatatct ttttgtatag aatataaata gtgctaagat ttattacatg ctgcatatac
     3301 aatatctata caacacataa cttgtaaatt ccttttattt tcatacgtct ttaaccgaca
     3361 tataattata ttcaataagt tttaaaatta tgaactcaat tataattatt tttatttttt
     3421 tagaatctaa acactaaaaa ttgtaggtaa atttgtctta tgcctttata aatatttcta
     3481 cagttttaat tactggacat atctattttt attataaaaa gcgattaaaa atggagaata
     3541 tcctcataaa aactatatat aagcaaaacg gttaaaggaa aaagaagaaa tttacttgaa
     3601 tgttaaaatg cgttgcttga agcaatgggc agttttgcct ataatatcgg taacaactga
     3661 aagaaaggtg gttagcccta atgttaagcg aaaacattaa aacaatcagg aagtcaaaag
     3721 gactttcaca acaagaactt gctataaaac tgaatgtagt gcgacaaaca atatctaaat
     3781 gggaacaagg actgtcggtt cccgattctg atatgttgat ttccatatca gaggtgcttg
     3841 aaacgcccgt aagcacttta cttggagaaa ctattgttga gtcggaagtt gataacttaa
     3901 aagtgatttc tgaaaaatta gagattataa acttgcaact tgcacaaaga aaaaacatga
     3961 gaagaaaaat gtttcattgg ttatttattt tattatgtgc agtcatatta atagtttttg
     4021 tggtcttagg gagactaaat agtccttact tggattggaa ttataccgac cctgaaaccg
     4081 ctgtttttgg agtagctttt catgcttttg agtggctatt tgtcagatta gtaccgattg
     4141 tccttatagg agcaattatt ggaatctttt tgacacgtaa gaaaatatga agtaactctg
     4201 ctttttataa tacagcaagc gttccttaaa agcgtcattc tacatggcaa tcgaggacga
     4261 ctatgtaagg aagaaccttt ttgatttcca actaagcgaa gttattgagg acgacagtag
     4321 gcccaaaacc gcattaagta aagtacagga agcaagactg ctggacttat actctcatgc
     4381 gtcagatgca ggaaatgcga ggtctggtag catgatttac aacgatttgt acaacgacta
     4441 cgagtagaaa tatatggaga taagtattat ctacgaaatc agaaaagaaa tgacttgata
     4501 atttactcat gttataatta ttgatgtgaa attaataaat cgaaattcct tttttggatt
     4561 taacaatact gagtaaggaa aaagtgaatt acttttgcaa ggaggtgtta tttggatggt
     4621 gctagcaggt attttgattg tagtacaagg ctttttgact ggtcctttag caaaaaaaat
     4681 tggtgagaag cgactaatac tgataggtat gttttgcaaa gccttagtag cgtttgccat
     4741 ggtattgaca actgggtttg ttagtgtgtt ggtggttttt ggcttatttg cggtatcttc
     4801 tgctattaca gtacccacat taaacgcaac cctttcaaaa actgacaatc agcataaagg
     4861 aacactgatg ggatttgcca gtaccgccgg aaatcttagc aaagttattg ctcctttatt
     4921 aactgggtat ctgtatgaaa gcaatattga attaccatat ataaccacgg gagggatagc
     4981 cttaatcgga gtggttatct gctatatctg gataaaaaat caaaagaaca aatgatggaa
     5041 gaggacgttt atatggaggt tatacacagt aactaatctg gtatagaaat tcaattgagg
     5101 cttttctgct ttattaaagt cttgctacta cttaacccta taatatcctt aattcattat
     5161 gacaaattcc aattcaacag ttttgataca cataaggagg taacaatact atggaaaatt
     5221 atcaatgaag tgctaattct ttatcagtga agttatccat aagtttttct acctgtaatc
     5281 gtctggtgct ttttgccaga tcattggtag gtaagaaaaa ttcatcaaca gacacatgaa
     5341 gtaatgatac aaggtcactt gtatgcttgg atgttgccct ttattttcaa tattagttaa
     5401 gtaacgtggg tcaatatttg taattcgatt ttagggaaat atttaaagaa atattttcgt
     5461 tttatttctt taaatcttca agaatattga tagttttttg aaagtaggtg tgtattatga
     5521 agaatgagaa aaaaattaca gtcgcattgc tcattgttta tttattgatt ttatcatgga
     5581 ttattttatt taagctacaa ttttcatttt ctgcgttaga ccacataaga caaataaact
     5641 taattccttt tggtggttca gttattgcaa atggaaaaat tgacttcgat gaaattataa
     5701 acaatgccat tgtttttatc cctgtaggtg catatttcag tttgcttttt aaaaataaat
     5761 ctgttctaaa agcaataggt tctgttttag gaattagctt tgtatatgag ataattcaat
     5821 ttatatttgc tatcggagca agtgatatta cagacttaat tagcaacaca ttagggggta
     5881 taattggaat tgcattagta tatgtattat ctattgttct aaaggataaa acacataaga
     5941 tattaaatcg aatagcaata gtatgtactg tattagtagt tgccttctta tttatgcttc
     6001 ttgctgtaaa taatatattt taatgccatg ttaattacat gttgttttgg aaaagacttt
     6061 aatctgtcaa agtttgcttt tgaaattgaa catgaaatta caccaatgga acaaatgaaa
     6121 gctataaatt tttcattgga gaaagatgac tgtacaagta aagtattaat atacaatcca
     6181 gacaaatgga aatatgtaga atactatttc tataaagata gacctattaa aaaaactatt
     6241 ggaaaaattt attcaatttt gcatttgtca caataaaata tgttttgaaa tattgaatac
     6301 ttaaatatgc aaaaaaacaa atgtaactgg ctatggggct atatctacta aaaagctatg
     6361 tgataggttg ggattaaatt taaacatcac ttctagaagg catgaataca caaggctgac
     6421 aaactataca gttatgatta aacgatacca acgggattga aaatttcgtg aagaactttg
     6481 agaagtccta ttacacatga gcaacagcaa cgaagctatt gaagcaagga cgcaggcaat
     6541 ctagcggtta tctgacaaag gagttgtagg acttgaatgt agatactatc agaacggata
     6601 taccgaccag ctctgcggtt acagatgtga ttgtgtagaa tatcgagcag tcgggaacgg
     6661 acactttatc tgctacctac gaagtagatc agcagataaa agagagagaa cagacaacag
     6721 cggtcaaggc aacctatacc gtaaaagtcc atgtggacgc tgacggggat atggtaatcg
     6781 ttcagaacct tactcttgca ccagcaatcg aaaaatcaga ctatgagcca aagatatcag
     6841 aaacagacgc aagcgtggac gctgataccg ttaatgacgc taccgcattt ctggaaacat
     6901 tctttgaggt gtacccgaca gccacagaaa aagaacttgc ctattatgta tcggaaaatg
     6961 tgattgaacc tatcggcagg gactaccttt attctgaatt ggtaaaccca gtctttacaa
     7021 aggacgggga caatgtgaaa gtcaaggtag ctgtgaaatt ccttgataat cagacaaagg
     7081 caacgcaggt gtcgcagtat gatcttgtac tccaaaagga tagcaactgg aagattttag
     7141 gataacatgg aaatgggctt gtatcataat tatagtgtgc aagccctttt catattgaag
     7201 tatcaataat caataattat aattaggtac atgaatggag ggataaaatg ttcaaaattg
     7261 gtgagttttc aaaattgaca caagtttcta ctcgtatgtt gcgttattat gatgaaacgg
     7321 gtttattaaa acctgctaaa attgattcat taactggata tcgtatgtat tcagcagaac
     7381 aaatctcaac tttaaataga attatatatt tacgtgatag tggcttcaat gtcgctgaaa
     7441 ttgcattagc cctcaatttc agtaataatg ccattataga acagcttgat agaaagtcta
     7501 ttgaaattat ggataatata caaaaagaac aagaaaaact aaagaaaatt gagattgcta
     7561 aaaaagaagt gttacatggc aaaagtgaat tgtattttaa cataaccatt aaagagatac
     7621 cgagttattg cgttttatct ttaagaaaag aaataccaaa ttattattat gagggcgaac
     7681 tctggaaaga actctctgct tttgctaaag aaaataaaat aaatatttct gatgaaacat
     7741 tctcaatata tcacaatgaa gaatataaag aaaaagatgt tgatgtagaa ttatgtgcca
     7801 ttgtaaataa aataggaaaa gctacaggag attttaagtt tcgttttacc gaaccagttt
     7861 ctatgatggc ttgtactatg gtatatggag atttttctaa tatttcttat gcttatcgtt
     7921 catttgcaaa atggcttcaa gaaaacagtc aatataagat gaaaggttca agccgtcaaa
     7981 tagttcatcg tggaccgtgg aacgagaaaa atccagagaa ctatctaaca gaaatccaaa
     8041 ttcctttaga aaatctcttg taaaaaatga taatctgcct tgactttaac atggtgttaa
     8101 ggtttatcgt ttatttatca agaagaaaac ggaggaaaat ataatgacaa cttataaagt
     8161 aaattcaata ggtactatct gcaacaatga aaaaggtgct ttcattcaac tagaagcacc
     8221 atatattcta gcattaaggg cattagacgg attcagtcat ataaatgttt tatggtggtt
     8281 cagtgatttt gataatacga attttagaag tgtattggaa acagaacagc cttataaaaa
     8341 tgctccagag aaaatgggga tttttgcaac aaggtcgcca gtacgcccaa atccgattgc
     8401 tttatcagca gtagaaatta tcgatattga ttatatgagt gggctgatac aaattgctta
     8461 tattgacgct aatgacaaaa cgcctatctt ggatattaaa ccatacacgc cgagtttgga
     8521 tagagtggaa aatccgcaag ttccagattg gtgtagtcat tggcctcaca gcttagaaga
     8581 atctgcttct tttgcatggg aaagcgaatt caatttttaa ggagtgatga accatgagtt
     8641 tgcaggacta catttttgaa aaaccagtaa ttgaaacaga acatcttatt ttaagaacaa
     8701 tgaataaaaa tgacatagat gatttgaaag aatggattag tgatatttct ttatacgagt
     8761 attgggggaa acgtccctca aaaagcgaaa aaaatccaga attactcttt gataagtcaa
     8821 aaaaatctac taaaagtttt cattggggta ttgtccataa acaagatagc aaagtgattg
     8881 gtgaaatatg gatatatctt attgaaaata atagaatggc aaaagtggct ttcagacttt
     8941 ctaagcatta tcaaggtaat ggactaatga gtgaagtgtt gaaaaacgta attgaatttt
     9001 gtttttcaaa aacagaatta caaaaaatat ggacagatgt gcatatttta aatcatgctt
     9061 cttacaagac attggagaaa gtcggttttc aaagggaagg acatattcga ggggggaaaa
     9121 tggtaaatac ctattgcgac tactacctat atggaatttt aaaatctgat tatatcacta
     9181 ggtggtgcta tggctaatat tgaagcgacg attggcgaag caatgaacaa tacagttcca
     9241 aaggtctatg atacgaaaag cattaaaaga tatgtgatga atttattaga tatggctcat
     9301 taaaaagtac caaagaaata aaagttacca tatagacaaa attggttttt tatatctttt
     9361 gatttcttat aagataatgg cattgtgcga caagctatgc aaaaaatttt catgtatact
     9421 ttgattataa tttatacaag gaggtactga tatgtacgca tatgaaaata ttgaaatgtc
     9481 attaaactat attgaacaac atttaagtga gaaaatcgaa acagagaaac tggcggaaat
     9541 tgcctgtctt tctacatttt attaccaacg cttgtttaaa aggttagtta agaaatcagt
     9601 tcaagagtat atcaaacttc gccgtttagc aatggtaata gcagaattga ataattcaga
     9661 ggaaagaata ttagacatag ctttaaaata tggattttca gaccatgcta acttcacaag
     9721 agcatttaaa gaagtttacc atattactcc cgatgaatac agaaagaatg ttcctatgtt
     9781 aaatacattt gacaagccag aactttcttc acgttatatc atgattgatg aaaatgtccc
     9841 attgattgta ggaaatattg ttttggaaat tcaaagaaaa acattaagaa caaaggaaac
     9901 atattttggt tttgaaaaaa cagtaaatat ttctgaacaa attcctgctg gagaaagtac
     9961 aggaattgat gttccaggtc agctatggcg cgaattccat gcaaagaaac tgcaaaccga
    10021 aaatgccatt tttaacaatg atattgaact tggtgtatct cacttagcaa gtccagataa
    10081 aggaacattc ctttactttg caggaggtat ggtttcatca acagatgtat gtcctaaaaa
    10141 tatggtacaa catacattac cagcaggtga gtatattgta tgcaaaattg aggcagaatc
    10201 ttttgaagaa cttgttacta ctgctttaaa tcaagcaaat aagtatctgt ttgaaacttg
    10261 gcttccaaat cataatttgg ttacaaagcc attcatggca gaaaaatatt acaaaggaaa
    10321 tgcagagatt aattatatgg aaatatggat aattcctgtg taaacagtaa atgctaaata
    10381 ggaattatct gacgtaataa aaaggaaata gaaagcttat aaaggagggc atagtccgtt
    10441 gtgataaaca taaaagatat aaattatgta cctaatatta gtgaaataag cgtttatatt
    10501 gaaaactctg catttgatga gttgtatcag catatgaaca atgaatataa agcagtttgc
    10561 aaaatcgaat acagtaaaga tgtctgggca cgaggttgga acgtaaagtt taaaaaatct
    10621 gggaaatcat tgtgtgttgt ctatccgaag aagaactact ttactgtatt ggtggttgta
    10681 ggtaataaag agaaagaaga tgttgaaaac ttgttaccta ggctttctga atatgtacag
    10741 gaaatttatc gtaacactag agagggaaat ggacaaaggt ggcttatgat agacttaaaa
    10801 tctaaaaata atgtatatca agatgtatta aggcttattg aaattcgtta tgccataaaa
    10861 tgtgtaaaac cgtaataaaa atatttttca catacgtaat ctcaaggtat tatcgttgag
    10921 acaagtaacc tatataatta tgggaaatga gatttttaga tacttaagaa agtactttag
    10981 ggggcttgcg ttatggaaag atttaagtac aattccacac aatcaaatac aatctaataa
    11041 aactttacta aaaattgaat gcaatacagc tttaaaggct cgtacaaact atatgttttg
    11101 tgcgggcttt tttcatttaa taggaatttg gattcgtcag agagtatatt ataaaaataa
    11161 aacgataagg gtaccttaaa aataaggtac ctcaggctgt agacaaagtc ccggactctc
    11221 gtctgggctt ttcttatatt tgagttatcc acaattcaac tttttaagag ttatcttatt
    11281 aagaaaaaag tgtatgtttt ccaccctata gttttacttc tgtgatattt gcagatgcca
    11341 ctcaagtaga aagtggtggt acggcagaag atgtcatgca gagttcagaa agtctgggac
    11401 taccgcctaa ttccttagac acggaaagct caatcaagca ggggtgtaag tattttgcgt
    11461 ccctgctttc ttcctgcaaa aatcaaggta tcgacgattt gaatgtagcg atacaatctt
    11521 acaactatgg cgggggttat gtgggctatg tggcaggaaa agggaaaaag cacactttta
    11581 atcttgcaga gagcttcgcc cgtgagaaat cgggtggaaa gaaagtaacc tacgccaacc
    11641 cgatagccgt tgcaaagaac gggggctggc ggtatggcta tggaaatatg ttttatgtgg
    11701 aattagtcaa tcagtattta actgttccgc aggtgtcggg ggaactggca caaaaggtaa
    11761 tgaatgaagc gttgaagtat caagggtgga aatatgtgtt tgggggaagc aatcccaaca
    11821 cttcctttga ttgtagcgga cttgtgcaat ggtgctatgg aaaagctggt atctacttac
    11881 cgagaacggc acaaacacag tatgacgcaa cccagcatat ccctttatcg caggcaaaag
    11941 ctggggagtt gttctttttc cattcaacct ataacgctgg ttcgtatgta acgcacgtcg
    12001 gtatctatgt gggaaataat cagatgtacc atgcaggcaa tcagcggtta tctaacaaag
    12061 aaattgcagg acttgaatgt tgatactatt agaaccgata tactgactag ccccacagtt
    12121 acagatgtaa ttgtgtggaa tatcgaacag tcgggaacgg acactttttc tgctacctac
    12181 gaagtagatc agcagataaa agagggagaa cagacaacaa cagtcaaggc aacctatacg
    12241 gtaaaagtcc atgtagacgc tgacagggat atggtaatta ttcagaaccc tacccttgca
    12301 ccagcaatcg aaaaatcgga ctatgagcca aagacaccag aagcagacgc aagtgtagac
    12361 gctgataccg tgaatgacgc taccgcattt ctggaaacat tctttaagct gtacccgaca
    12421 gccacagcaa aagagcttgc ctattatgta tttggaaatg tgcttgaacc tatcggcagg
    12481 gactaccttt attcagaatt ggtaaaccca gtacttacaa aggacgggga taatatgaaa
    12541 gtcaaggtag ctgtaaaatt ccttgataat cagacaaagg cgacgcaggt attgcagtat
    12601 gagcttattc tacataagga tagtaactgg aaaattatta tgtaagacat tttgttggca
    12661 ggatctattt tcggatatag tctgcctttt ctgtagcaaa tgtactgcac tgacaagcta
    12721 tttcattgta ttttaagcaa ttaaaccttt cttgacaatt ttatcttaaa tcatatattt
    12781 atggatattg actttctgct tgcaataagc tataatatga ctataaaatc aaatcagtca
    12841 tataggaggt atgtggtatg tataaagaag ttggtatttc ttcaaataat gttccgataa
    12901 cactttctat ctggtataca gaaagaagta gtcctaccat tgtttttttg ccgggtacta
    12961 tgtcacaccc tctcatgtat gagaattttc tatgtggttt gagtgaaaga ggatttaata
    13021 taataggcat tcactatctt tcacacggaa aaagccctaa aatcaaaaaa aattatacaa
    13081 tggaagattt gctgcataat gtatatgacg caacaactta cggaatagaa aatttcggtg
    13141 aaaacatagc tgtcatgggt tccagtcaag gcgggatttt agctgcaatg gcggcaggta
    13201 gggacacaag gttaaaagcc gtttttccac ataatatcat gctgaccaca ttaaaagaaa
    13261 ctatgagctt aacaaaatat cccacatggt cgcaccgttt tatggggctg atacagtggg
    13321 gctttcgtgt aggtggaaaa ttgctgccta actacaaggt tccaggagac gcttatttag
    13381 actatgacag agtattttat agtgaacagg caaaacaaga ttgcttaaat gaccctatgc
    13441 ttttacccta ttatccttta agatttgtca ccagcttatt taatgctgac ttatcgtgcc
    13501 tggagaatgg ggctatacaa tgtcctgtta ttttaattac tgccaaaggc gacccgcttt
    13561 ttagctttga ttataccaac aaagtatttg atttaattca tgtaccgcaa aaggaattac
    13621 ttgctttaga gcttaatcgt cacatgattt ttaatgagga cacggacact acaataaacg
    13681 cattggaagc taccttgcat aaatatcttg gatagcatat tgattatata atcagtaaat
    13741 gttatacttt tcatgacgat ataaatattt tagtcataag gaggttatga gtttgccacg
    13801 atttacggaa caagaaaaag aaatcataaa cagtaaactt ttgatagagg gagaaaagct
    13861 gtttgcatta cacggcttaa aaaaagtaac agttgatgat ttagtagctg ctgtaaacat
    13921 atcaaaaggt tctttctatg ctttttatcc cagtaaagaa catctatatg ttgaaatcaa
    13981 ttttcgctta caaaaagagt tatttacaag tatagaaaca accattaaaa agaaaaagta
    14041 taagaaccat agagatttag caaaggacgt tatcatgcta agtttaacgg gggtgattac
    14101 ttcgcctatt ctttcacaga ttgatttatc tttaatggac tatctgcaaa gaaaaatatc
    14161 gtcagatatt tttgaaaatc atatgtacag tgatattcgt atattggaga tattggaaga
    14221 tttaggggtt gtatttttgg ttccgcatac ggttattata aaatccctgt attctgtctt
    14281 atcctgtttg gaacaattca aagaggacga agaactgaat atgatacaaa atcttttagt
    14341 gaatggtatt attcagcaag tagtagcaga gtaacaattt tgtttggtta tttgacaggc
    14401 aggtagccaa acaaattttt tacagtttat atgactatat atctataaac gtcataaagt
    14461 taaaggagga atgaataatg attgatgtaa aaaaactgta ttttagctat actgacaaac
    14521 cgtttgtgga gaatgtaagt ttccatgttg ggagaggaga aatttttggt tttttaggac
    14581 cctctggtgc tggaaaatca actattcaaa aagttttaac tgggcttaat acaagatata
    14641 aaggcagtgt aaaagttgca ggtacagaaa taagggagca tacaaatcag ttttatgaaa
    14701 atattggggt agactttgaa ttttcaacct gctacgagaa attcacagca cgacaaaatc
    14761 tcgcctattt tgcttctcta tatgaaaagc aacctcggtc tattgatgaa ttattgcata
    14821 tggtaggctt ggaaaatgac ggggacaaaa aagttgctga cttttccaaa ggtatgcgtt
    14881 ctcgcttgaa tttcattaaa gcgttagtcc atgaccctga tatattattc cttgatgaac
    14941 ctacaagcgg tcttgaccct acaaatagtc gtttaatgaa agatattatt cttgcggaaa
    15001 agaaacgcgg aaaaacgatt atcattacta ctcacaatat gtttgacgca acagagctat
    15061 gtgaccaagt agcctttatc gttgccggaa aagtcagtgc attggatagc ccgcataatt
    15121 tgattatgtc aaggggagct gccaaaatac aatataccta ctatgataaa ggcgagaaaa
    15181 ccggagaatg tctgcttgat agaaccacag aagataagtt gttaaaaagt ttaatttccg
    15241 aaaaccgttt gctttctatt catagtagtg agcctacatt aaatgatatt tttgttgata
    15301 ttacagggag gacacttcaa tgagattggg acgtttaatt tgtggtgata tacatttcca
    15361 atggaaatat ggtttctatt ttatctactt tatattaaca gttttatatg tgtgcgggat
    15421 tgccgctttg ccggaacatt ggaaaacgga tattgcttct attatgatct actctgaccc
    15481 ggcagcaatg ggattatttt ttatgggagc gattgtcctc ttagaaaaaa gtcaaaaggt
    15541 gttaaatgct atggtggtat cccctgtaaa aatatcggaa tatatacttt caaaaacagt
    15601 tgcgttgatt gctatttcta cggtaatcgc attgatatta ggggtagttt ccggcagcaa
    15661 tcatttactg ggtatcgcag ttggtacggc tttgacctca gcaatcttta ctatgcttgg
    15721 aattattgca gctacaaaaa tttcaaactt gaaccaattt ctcattgtga ttatgccgat
    15781 agaaattatt tgctttgtac cacctattgt tggtctgttt gtgaagttac catatctgtt
    15841 ccgctttttt ccattcacag cttgcatgaa ccttataact ggaaaaagtg ttttactatc
    15901 gtttgatatg gtacttgtaa ttgctacgtt gattatcctc tacatagttg ctcgacatac
    15961 agttgagcat atgtggaaaa gtttgggagg tgtaaagcta tgaaaaagat attatccagc
    16021 accatacaga tatttaagca aatcaaaagt gaccctatga tgtttgcggc ttgcttctcc
    16081 ccttttatta tggggacttt aattaagttt ggtattccat ttcttgaaag aataacgaag
    16141 ttttctttac aagcgtatta tccgattttt gatttattac tttctatcat ggctcctgta
    16201 ttgctctgct ttgcatttgc aatgattaca ttggaggaaa tcgacgataa agtgtcacgg
    16261 tacttttcga ttacccctct tggtaagtcg gggtatctct ttacaaggtt gggagtaccc
    16321 tcaattattt cggcggtcat cgcttttatt gtactgttac ttttttcatt agaaaagttg
    16381 tccattggaa tgacaatatg tttggcgctt ctcggctcag tacaggcaat tattgtttca
    16441 cttatgatta tcacattatc aagcaataaa ttagagggta tggcagttac aaaactagct
    16501 gcacttacat tgctcggaat accagccccc ttttttattg atagttacta ccagttcgcg
    16561 gttggcttcc ttccatcatt ttgggtagcg aaagccatgc agaatgaagc ggttctttat
    16621 ttctccatag gattggtggt agctttagtc tggtactact tccttacaaa acgtctgttt
    16681 cggaagctgg caggataagg aggcatatta tggaaaaagg tagacttctg caaaacaaaa
    16741 atatgaataa ggctattcag gtttccaaaa ttacactaat catcaatctg agtttatcgc
    16801 tacttaaatt tgctgccgga tatataggaa agtcaagtgc catgctgtcg gacgctgtac
    16861 attcggcttc tgatgtagta agcacaatcg ttgtgatggt tggaattaaa atatcagaaa
    16921 aacagcctga tagagaacat ccttatgggc atgaacgtat ggaatgtgtg gcttccatta
    16981 ttctctctgt tgcattggct ataacgggtg ctggaatagg atattcaggt atcaagaaaa
    17041 tcttttcagg acagtacaat acgctttctg tccctagtgg aatcgcactt acggcagcag
    17101 tcttatctat tgttataaaa gagtggatgt actggtttac aagaagtgct gcaaagcata
    17161 caaattctga cgcacttatg gctgacgcat ggcaccaccg ctctgatgca ctctcgtcgg
    17221 ttggttcctt aattggcatt ttaggagcgc gtttaggcta tgctattctc gatcctattg
    17281 caagcgttgt gatttgcggt tgtatcttaa aagctgcttt gaacattttc aaagaaagca
    17341 ttaacaaaat ggtagaccat agttgtgaca atgctactga aacaaggatt agggaagttg
    17401 tgcttcagca gcaaggggtt gatggtattg atgaattaaa aacccgcatg tttggagcga
    17461 aaatgtatgt agatattgaa atattagctg atggtaattt agctctttat gacgctcata
    17521 gaattgctga gggagtccat caaacgattg aaaacaactt tccacaatgt aagcattgta
    17581 tggtacatgt taatacgaat gaacttctct attctgcata ggacagggaa gatgatttaa
    17641 ggctattgaa agggaaagga gggaagtagg ttatggaaca atactggtgg attatcattg
    17701 ttgtggtttt gattgtcgtt ggcttactta aaattgtaat tatgaagaag tataaccaaa
    17761 ggagaaattc taacaatcgt tcaaaagata gcgaggacga caagatgtat taaggtttat
    17821 tgaaatctgt tatgttgtag aatgatgttt ttagatacta gaaagtgctt taaggggggc
    17881 taacgttaag gaaagctttg agtacaattc tttacaatca acagtctttt gcatagagaa
    17941 atgtatgtag gaaatatggt gcaaggcaca aaacaccagc aattgagaaa tcaacctatg
    18001 aaccaaaggc acactacatt ttatactact tgacaagtaa acgaacattt actttaatgg
    18061 tttaaagaag taaacgttcg tttactaaag aaggaggttg taaatgtctg acacaaaaga
    18121 aaatatatta aaaattgcac tacacttatt tgcagtgaat ggttatgagg ctgtttctgt
    18181 tagtatgatt gcggaagcct tgggaatgac aaaaggagca ttatataaac attataaaaa
    18241 taagcaagat atttttgact gtatcgtaga acgtatgtat caaattgata ataagcgagc
    18301 taaggattat caagtaccag aaagcacaat agaggtaacc ccaaatgcat acagaaatac
    18361 aactattgga agtctaataa catttactct tgcacaatac gatttttggg tgactgatga
    18421 atttgcgtct aattttcgca aaatgttaat actagagcaa tatagaaatt taaaaatgaa
    18481 tatgctttat caaaaatgtc ttgtaaatgg tcctatcagt tatatagaag atatatttaa
    18541 tgaaatgatt gaaaaaggtg ttcttcaaga gtgtgataca aaacagcttg ctattgaata
    18601 ttatgcgcca ttatttctat tgataagtgt agcagacgca gttgaaaatt gttgggattt
    18661 aaaaactcaa ttaactacac acgttaaaca atttgtcaag aaatatatta taaatgagaa
    18721 agtaaaataa tcctttattc tgtcctaaat ataaacctaa ctttttaatt tcagcagaga
    18781 aactcaaaat gaatattgtt ataagtcaca aatattaact attaaaatat tacaataacg
    18841 gagatgagaa aatggaatat attcgcagta aaaaatattt tacacccgac ttacagacaa
    18901 aaattatggg gccgaatcct ataaaattac aagaggaact attacaaaat cataaaatcc
    18961 cgtctggtag ctatgtctgt gaccttggaa gtggacaagg tttaacttct gtatttcttg
    19021 caaaagaata tggatttgtt gtttttgcag ttgacttatg gagtgagcca aaagaaaatg
    19081 aagaattttt caaaaaaatg ggattatctt ctgcacaaat taaatctgta aaagctgacg
    19141 ccaatgaact taaatttgag aaagagtttt ttgatgctgt agtaagtaca gattcatata
    19201 attattttgg acgtgaccct atatatttag atgaaaaatt gcttccattt gttaagaaag
    19261 gtgggtttgt ttatatcaca gttcctggca tgaagaaaga ttgccatgag aaacttccag
    19321 aagagttatt gttatcatgg acaccagaag aacttgatta tatacatgat ataacatatt
    19381 ggtctaacat aattagtcat tgtaaaaagg cagatttaat ttcaatatat gaaatggaaa
    19441 gtaattcgga agtttggtcg gattggataa agcaaaataa tgaatatgca ataaatgacc
    19501 gtaaggcaat agaagctggc ggtggagatt atctgaattt tattgctata attctacaaa
    19561 aaaaataaat tctttagaac caaacactat aatttgcgtt ctaatatgtt taccgattga
    19621 catcaataat tttatctttg agggtaatga tttgaaaata gaagaataca agaagatacg
    19681 aaattgataa attagaattt gatgggatgg tgatattatg aaaatattat ggatcattat
    19741 tattatggca gataatctgc tgccgttact gcttgctcgt ttctatccaa actataagca
    19801 taaagaaatg gcactatctg tccttggctc tcgtcaaagc ccggtgaaat ggatatataa
    19861 tgtgtggtgc attatatcag gtgtggtatt ttgtatcgct ccatatgcgt tgtatcaaga
    19921 gaacgatagc ggatgggcaa tcgctatatg gatgcttctt gctatatacg gcatcgggtg
    19981 tgaaatcatt tccggagtgt gcccgttaaa tgaaaataga caagacgaag atataattac
    20041 aaaaattcac ggcggcacat cggcaatcgg atttatggcc ttgcttgtcg ttcctttgct
    20101 ctttgcaatt ctgcaattcc aaacaacaaa agtagtgtta ggcgcaattt cactggtgtg
    20161 ctttacaata gcattcatat tcttctgctt ttttattatg ggtgagaagg aaaagtttgc
    20221 aaatactgtt ttgagatatg gcggtctttg gcaaaggctt gttttaataa gttgttatat
    20281 accgttattt gtttggtctg tttctaatca ataaaattta gtttatagga ataacaaatt
    20341 ccagtttgta aaagtcaata aatccaaaga taaatttgta tttgaaagga gttttgaaga
    20401 atgaaaaaat atgagtatgt agaaattaac tataatgcga aaggagttgt attcctttgt
    20461 actgataaac acaaagaaat tattgacagt tacgcaaaaa cggggtatca ctacgcaggg
    20521 tttgtaccca cagaaattga cgcaaaaggc tgtatgcgaa agatagattt aatttttgaa
    20581 aaagaagatt aacttatttt agggagttgg ttctaatgat aggacttaaa agacgagata
    20641 tgaattatat tatgaaagtt ggtgctaaag ctcaaggtgt ttctgtatct gaatttaggg
    20701 ctatgctaca agataccata gacaaaacta tggaaagtac agacccagac gtgcaggcaa
    20761 attttaaacg atactttgga aataagcgcc caacaccaga ggaatatatt tatacgatta
    20821 ccaaaaagac aaaggttaag gtttaaatgt tgtaaagggg gcttgccgta tgaaacgctt
    20881 tatgtacaat tcagtatatt caagcccaat ttaacagcat actatttaca attaaatacg
    20941 ctataccagt agctcgtaca aaatttatat tttgtgcggg cttttttaat acccgaaaag
    21001 tttttttaat tattttcatt tttttggaag caaatcacac cttgctgtac agatatggtg
    21061 cggaaagagg gataaacact tttcaattca tagctggaaa ggaggtgaga ttatgaaacc
    21121 gtctgacttc caaaaaacaa ttcagtgtca gtttgaatgt aagctgaaaa aagttgtcaa
    21181 aggtattatc tggaactacc gtaaagaatt aaaacgaaga aaaaacaagg aaatatcttt
    21241 tagcgaatta ccagacatta cggtagaaag tctggcaaca tgggacgact acgaaacaaa
    21301 ctacacaatt tttaatgtgt gtggcataga tattaaagtc cttgatgatg aacttgcaga
    21361 agctctcaaa cagctacccg aaaaaaaacg taacaatgta ttgatgtatt acttcttgga
    21421 aatgagtgat acagagattg cagatattat gaattatacc agaggggctg tgttcaaaca
    21481 cagacaccgt gcactagaaa ctatgaaaga gatactaaag gagaagaaat agaaatgaag
    21541 aaaaagtaca agcacccgtc ttattatttg atttcatcag ctatggatgg ggatgaaact
    21601 gcgattgaga aattactggc attctatgac ccgtacatat caaagtgttg tttacgtcct
    21661 ttatatgatg aatacggcaa tgtttatatt gttgttgata tggaattaaa gggacgtatt
    21721 cgagaagcct taatcaaaat gattttagac tttgagattg atatagatat agaaaccgaa
    21781 gaagaataag caaataccag cagggcgtga ttggttaaat tcccttcttt cctgccctgc
    21841 tcctgctttt atacatggaa gcctacggct ttcgccgatt gttctttgac aactgaataa
    21901 agcagacaga tacgtttgtg atacagcgag ccacggggac tgtacgccat gacctttccg
    21961 acaagaaagc gagcgaccca cgcagttgat ccgagagcga cttgtggaat ggtcgttttg
    22021 ccatgacctg ctatcacaga ataatgatac gtccgcatgg tgcggttctg cccacaagaa
    22081 tgggaatagt tgagatacta acggagcttt ccaaagccgt ctgtctgctt atgataagac
    22141 acacagaaag gagttgttgc aacatgaaca atactgatgt gccgatttgg gaaaaataca
    22201 cccttagtat agaagaagcg tcccgatatt ttcgtatcgg ggaaaagaag ttgcgtaaac
    22261 ttgccgagga aaatctggac tctggttggg ttatcgttaa tggaaaccgt attcagataa
    22321 agcgaaaaca gtttgaaaaa attattgata cactggacga aatctgatgt cttagagccg
    22381 tagatatggt ataatattag tagcgtatca cggctcattc catgacggaa aggagcttta
    22441 cactatgtca aaaatgaaaa gacaagacag taaaggacgc aatttgcgtc ttggagagag
    22501 ccagagaaaa gacggaagat acgtttataa atataccgat atattcggga agccacagtt
    22561 tgtatatgct tggaaacttg taccgacaga caaaacacct actggaaaac gtgatgataa
    22621 atcattgagg gaaaaagaaa agcagataaa aaaagacctt gacgacggta tcgacactat
    22681 cggcggtaaa atgacagtct gccagcttta tgcaaagaag aacaaccaga aaaagaacat
    22741 caagagaaac acggaaatag gacgacagta tcttatgaac gcactagaaa atgacccatt
    22801 aggtatgagg gcgattgata cagtcaaaca gtccgacgct aaagaatggg ctatcagaat
    22861 gaatgacaaa ggttattcct ataagacaat agataactat aagcgttcct taaaggcttc
    22921 attctatatg gcgatacaag acgactgtat cagaaaaaat ccttttcatt tcaagctaag
    22981 tgatgttctg gaagatgata cagagcctaa agttatcttg acaccagagc aggaagaaaa
    23041 acttctttcc tttatggaaa cggataatgt ttacagcaag tatcgtgatg aagttattct
    23101 tctgctggaa acgggacttc gtatctctga attatgcgga ctgacaacac acattgatat
    23161 gcttaatcgt gtgattaaca tagaccacca gctattacgt gatacagagg tgggctacta
    23221 tatttccaca cctaagacaa aaaacggcaa gcgagagctt ccattgacag aaagagcata
    23281 tcaggcactt gaacgaattt taaagaaccg aggaaaagct caaccgcttg ttgtagacgg
    23341 ttatagcaat ttcttgttct tgaaccgtga gggcttgcca aaggtagcag gaaattatga
    23401 aagtatggtg cgtgggctga ttaagaagta caacaagacc catgaggaca agttgccgaa
    23461 catcacgccc cactcattca gacataccta ctgtacgaac atggcaaata agggaatgaa
    23521 ccctaacacc ctgcaataca tcatgggaca cgcaaacata accatgacat tagggtatta
    23581 tgctcacggt actttccagt ctgcaaaggc agagttggaa aggctggctt cttaatatcg
    23641 aaaatgtatc ttactactta tttactactt ttggttgcat tttcatgctg gtaaatgcca
    23701 gcttatgcga ggtatcttcc aaaaagaaaa tgcccataca gcccgaaaat acgggatata
    23761 tcggcattta ccaacttatg aaaagataat cagaaattag taagttttta tatcaaaaaa
    23821 atgattactg taaagaaatc attttttatt ataatcactg atacatcaaa tattctaaat
    23881 aaaattttta tttatctgga attgtatatt ttttctataa tattaactac agcgtacatt
    23941 gcccaagcac agatagccaa tacaaatact cccatcatta ctaaatctaa tttaaatact
    24001 tgagaaccat atactatcaa gtagcctatt ccatatcttg aaactaaaaa ttctccaact
    24061 attacaccta cccatgccat accaatatta attttagtta aatttattaa atttcctgtg
    24121 tttgcaggta atatgagttt aaataatatt tgagatttag tagctccaaa actttttaac
    24181 atcttaattt tttcttcatc tatattcata aaataattat aagctgaaag tatagtaaca
    24241 acaacagaaa tcgttacagc tgttacgacg attccttcta ttccagcacc tacccaaact
    24301 ataattatag gagctaaagc agtcttagga agagcattta aaactaccaa aaatgggtct
    24361 aatatttttg ataatttttc tgaccaccaa agagcaattg ctaccaaaat acctaataca
    24421 gtacctataa caagaccaag aacagtttca tatacagata ttccaatatg tttaaataat
    24481 tgaccactaa atgcatattg aataaataaa ttgtaaatat cacttggttt gctaaataga
    24541 aatgtattta taacatttaa gtcagctaaa agttcccaga ttacaataaa accaattaaa
    24601 attaatagct ggtagaataa tacatttctt ttttctcttt ttacaccttt aacatagttt
    24661 atatatcctt cagatctttt attaagcctc atgtgtatct agctccttcc ataatatgtc
    24721 gaaatacttt tgaaaatcag gaactttacg tgttttgaga ggcgtttttg ttccatccat
    24781 gttaagttca atatcgtaaa tattttttat ggtagctggt cttttagata gtattgcaac
    24841 cttatctgat atgcttattg cttctgctat agctaaaaaa gtgcaactaa ttgcttaaag
    24901 aaacagaatt atcaacttca gcacccatat aaacaatatt aagatttcct aaactgctat
    24961 cccaaacgat actattaata atagtagata ataacaatct cttatcatct aaactggcat
    25021 tatcaacttc atcattaaat ctcttcaaat tactaacaat aatatcaata ttaatactgt
    25081 cactactaat attgatgtta ctagattcca aaatattaat cttatccttt aaaatactaa
    25141 tctcattatt taggctctca atctgggaaa ctatataatc agaagcagta ctaccagtat
    25201 tttttgcaag ctctataact aaatttttaa taagtctttc cttatctaaa atttgatttt
    25261 taagtaaatt agtctcagat ttaaaaatat taaaaacatt atcagtctta atgttattac
    25321 tattgaatgc acttataata gactcttctt tacaattctt aattttatct ataactaaac
    25381 tttcaactaa tggaccatta agatttttac agttgcaact atcagaccca agtgtttttt
    25441 tagtaccaca aatataataa tactttaaat ccttgtcagt ttttttgcct ttataggtaa
    25501 ttctcatatt agaaccacat ttaccacatt ttataagacc agataaaaga gcagtttttc
    25561 cagttgatat ccttggagtt ttatcacgat ttttatctaa ttgattttgt acttcaagcc
    25621 atatttcagg ttctatgact ccatggtgtt ttgcaattgc tgctattgag ttagatgtat
    25681 ttttagcata ggtaaggatt ccacattttc tatctggagt acctacaaca tccataccac
    25741 tatttttaag gtaatctaaa atttcatcat tagcttttac atatgctgga tttctaagta
    25801 ttctagataa agaactaggg tcaaatttgc caccacgagg accccttatt ccattattat
    25861 agacatattt atataactta gataaggacc taagttctaa atatttattg tatattagct
    25921 ttacaatctc catttgttca tcatttactt gaagcttata catacttctt tcttttagat
    25981 tttcatctaa gaatgtgatt ttttcactgt tgaatccatg aggtgtattg cctccaagcc
    26041 atctaccact acgggctaat tcatacatat tgtcttttat acgttctgct attgtttctc
    26101 tttctaattg agcaaataca cttgatataa acatcatagc tcttcccatt ggagttgaag
    26161 tatcaaattg ttctttgata gatataaatg atatgcttag ttcatttaat ttttctatta
    26221 aagtagagaa gtcagataca tttctcgata tcctatctaa tctataacaa actaggtaac
    26281 taaatttctt atctttggca tcctgcatca ttttcttaaa ttgtggtcta tctatattgc
    26341 taccactaaa tccctcatct tcatatatca aaaaatcttt tacaccaata gactttgcat
    26401 aatcaataca taattgtatt tgattttcta tagaatcacc ttttccagta aatcttgatt
    26461 ttcttgaata aattgcagct ttcaaaatta aaacccccta taagttagga tatatatttg
    26521 aaactattta tctacaacct tttaagtaaa cttattagaa tgaatttatt aattaggtaa
    26581 atttatataa ttctactttt tataatatat gactatttat tttatttctt tactattcaa
    26641 gttctaaatc aataataaat tttgatattt aattgtatca ggacaaatct ctaatttcat
    26701 agattattaa tataaaatca taggttagtg tgaaaaagtt gaaatttaaa tattttaaaa
    26761 gataattttt ttgaactaat aggtataaca aaaatttata gggggattgc atatggaaat
    26821 caatgttata gaaattttcc ctaaagataa agctaaactt aataaaatag aaatggataa
    26881 agctagttgg tttgtaaata taataagtaa aaaatatcct aaagaagctt taaatgaagc
    26941 atttagtact ttagaaaaag aattaaatat aagtaaagct aatacataag ctttgattat
    27001 tgattttata tactattggt tttatacgct atgacttgac attatattaa tcaaatagta
    27061 tatttttaaa tatagcacaa tgaagtgtta aaagagcaat ggagctccat agataaagtc
    27121 tatggagctt ttttgcgtat ttaacttaag gaggatagga ctttgaataa ctttgagatt
    27181 aaaactagaa taaaatttgg agaaggctca ctaaaagctt tgcaagaatt aaaaaacaaa
    27241 aatgtactaa taataacaga cccttttatg gtgaaatcaa aaacaataga taaaataaca
    27301 tctaatttaa taaactcaac atatcaagtt tttgaagaaa tagtaccaga cccaccaata
    27361 gaagtagtag tagcaggaat agaagcattt aaatctataa atccagacgt aataatagca
    27421 ttaggtggtg gttctgcaat agatgcagca aaatctataa tggacttttc taaaaaaata
    27481 ttaaaaatta acgaagtaga gtttatagca ataccaacaa caagtggaac aggttcagaa
    27541 gtaacatcat ttgctgtaat aacagatagt caaaaaaaat gtaagtatcc attagtgagt
    27601 gatgatttac ttccagatat ggcaatacta gacccagaac ttgtaaaaac tgtaccaaat
    27661 tttataacag cagatacagg gatggatgta ttgacacatg ctatagaagc ttacgtatct
    27721 acaaattcta atgacttttc agatgcacta gctgaaaaag ctataaagtt agtatttaaa
    27781 tacttaataa aagcatataa agatggaaat gacatagaag ctagagaaaa aatgcacaat
    27841 gcatcttgtt tagcaggtat ggcatttaac caagcatcac ttggcataaa tcattctatt
    27901 gcacatgtat taggtggaaa attccatata ccacatggaa gaacaaactc tatattactt
    27961 cctcatgtaa tagaatataa ttcatcaata gtaggatata ctaacacaac ttactctgct
    28021 acagctaaaa aatatgcaca aatagcagaa ttagtaggac ttgggagtgc taatataaga
    28081 atatctgtta gaaatttagt aaatgaaata aagaaactac aaaaagaaat gaatatgcca
    28141 actaaattga cacaatgcaa attaagttta gaagatataa ctaatgaaga aagagaaata
    28201 gcaaaacttg ctataaaaga tacttgtaca ttaactaatc ctaaggtacc aactgaagat
    28261 gatataataa acatattaaa gaacattaaa taactacatt ttattacatg taaatataat
    28321 attatacaaa tagggaaaat gtttttaaaa atataaatta tatattgaca aatataaaac
    28381 ataaaactat aatgaaagta tcacaaaggc gtgatacttt agtagaaagc aatggagctt
    28441 cttaggaata ttaatattat tcttatggag ctccttttat tttaatcaaa tagcttttta
    28501 ataaactata ttcaaagaaa gcagggaata aatttgactg aagaatctaa gcaaagagtt
    28561 attcaagaat atgttcctgg aaaacaggtt acattagcac atatcatagc taaccctaat
    28621 gaagatatat ataagaagtt aggattagta cttgataaaa aagatgcaat aggaatactt
    28681 acaataacac caagtgaagc gtcaataata gcagcagatg ttgctacaaa ggcttcaaat
    28741 gtatctttag gttttataga tagatttagt ggttcagtag taatatcagg tgatgtaagt
    28801 tctgtagaat cagcgttaaa tgatgtttta gaagtcttag gaaatatgtt aaacttctca
    28861 tctacaaaaa tcacaaggac ataataatac aatgaagaat ataatattta tgggaaagac
    28921 aggtagtggt aaaactacct tatgtcaaaa actagaccag ttagaattaa agtataaaaa
    28981 aactcaatca gtggaacttt ataataatgc aatagacact cctggtgagt atatggaaaa
    29041 tagaaaccta tataatgcct taataacaac atcagcagat gctaaaatta tagcaatagt
    29101 gtatgatgcc acacaagaag aaaattatat agcaccaggt tttgccagta tgttttgcaa
    29161 agacgtaata ggaattataa ctaaaataaa taaagctact gaaaacgaag tcaatatagc
    29221 ttatgaaaga ctatcactag caggagcaag tcaaatcttt aaaatagaca cagttgataa
    29281 tattggactt gaaaaattat ttaaatatct agaaaaatat aaaaatatag atgaagttta
    29341 aggggaaata tttttttatt caaaaatatt attaaagtat gataaaataa aaaaatgtat
    29401 aaactatttt aagaagattt aatctttaag cgaggtagga acatggggaa aaaaatacta
    29461 ttggttgatg atgaaccact gttaaggatg gatacaaaag atatattaga aagtcatggc
    29521 tatgaaataa taggcgaagc aagtgatgga tttgaagctg ttgaaatgtg taaaaaacac
    29581 aaacctgatt tagttataat ggatatagag atgccaatac ttgatggtat aaaggcaggc
    29641 aaaataatat gtaaagaaaa tatagctggt ggagtacttt tattaacttc atttgaagat
    29701 aaagaatacg tagaaaaggc taaaagtata ggtgcttatg gatatttagt aaaaacagca
    29761 agtgaaaaat cacttatccc aaccatagaa atgtgtttaa ataaggtaaa tgaatttgaa
    29821 aaaatgaaaa aagatttaga aaaagtaaat tctaaattga atgaaagaaa attagtagaa
    29881 agagcaaaag gtatacttat aagagagttt aagatttctg aggatgaagc atacactaga
    29941 ataagaaaat taagtatgga taaaagaact acaatgatgg aaattgccaa aatgattata
    30001 ataggatacg atgactaata cgataaaaaa aatatcaact gcacaaacat acttaaataa
    30061 taacgaaatt gaaaaaatta tagaagtatc aaaatcctta gatttaatgg cttcttttta
    30121 tgaagctgat gtttttatag atgtactaag taaatttgat gatgaagcaa ttgttgtggc
    30181 acatggtaga cctaaagaaa agtcaatgta ctgtgaaaat gttataggta aaaaggcttt
    30241 aattgaaaat gaaccaggag ttataaagtg cttaaaaaca ggagaaagag taagagatat
    30301 taaagcttta acacaggaat ataaactggt caaacaaaat attcagccaa taacactaga
    30361 tgacaaagtt ttagcagtac taatagttga aaaagatata agtagtgaag ttaagaatga
    30421 gtttaatgct agtgaagacg aagaaaataa aaatttacat cagtttataa aacttattgg
    30481 aaataatgac tttttagttg ataatttaaa ttcagccata ttaatatttg ataaaaatgg
    30541 aaaattaaaa attaaaaatc gtattgcaac tgagatatac aaaaatcttg ggtttggata
    30601 tgatatacaa gatatgcatt acaatgattt agatatagaa accaacacct ttgaaaatat
    30661 attaatggat gaaaattatg atgagacaaa tgaagtttca ataggtaata tgttttttaa
    30721 aataaagacc atatacatca aagatgatga atttaaggtt tgcaaaatag tacaagatat
    30781 aagtgattta aagagaaaag aagaagaatt agttttgaaa acagtggcta taagagaagc
    30841 tcatcataga gtaaaaaata atcttcatac agtaatatct ataataaaaa aacaaagtag
    30901 attatctcaa aatgatgaag taaagcagtg tttagataac ataataaata gggtatttgc
    30961 aatactttcg actcattatt tactatctaa agaagttgat aataatatat ctatacgaga
    31021 aggtatcaat ttattagtgt caaatattca aggtggctat ttatttagta aggatataga
    31081 tatacgtata actggtgaag attttaaaat tagtggagaa aaagcaacag caatactttt
    31141 ggttataaat gaaatactac aaaattgtta tgaccatgca ttttcggata gagaacatgg
    31201 tactatacaa atattaataa ataaagataa cgactataga aatattgcta ttatagatga
    31261 tggaattgga tttgatagta ataatataaa taaaaatagc ttagggacat atataataga
    31321 tagttatata acacagatgt taaaaggaaa tatagagaga aaaagtagtg agaatggtac
    31381 agaagtatta attactatac ctacacaaat taatatttaa ataataactc atctacaaag
    31441 tagtagtttt aattgagcaa agaggcttaa ggttattaat agattaagtt aattgaatgt
    31501 atttataaaa attataagta tatattcaat ttaacaggta tttattataa ctttaagcct
    31561 ctttgcttgt ttaagacata cttaaatttt aggaggtgag aaaatgagag aagagttatt
    31621 aagtgttggt attgacattg gaacaagtac tactcagtta attttttcta aattaatagt
    31681 agaaaatact gaatcaaatt tttcggtacc tagaataagt atagtagaca aagaaattat
    31741 ttataaaagc aaagtttatt tcacccctct tatttcaaat aatgaaataa atggtcaaca
    31801 aataaaagat atagttgaaa atgaatataa aaaagcaaat atatctaaag aagatataca
    31861 tacaggagca gtaataataa ctggtgaaac agctagaaaa gaaaatgcaa atgatgtact
    31921 tcatactttg agtggatttg ctggggactt cgttgttgca acagcaggac ctgatttaga
    31981 aagtataata tcaggaaaag gagctggagc tcatatttat tcaaaagaac attcaactac
    32041 aattgtaaat atagacatag gtgggggaac tagtaacata gcctcattta aaaggggaga
    32101 agtaaccgat actgggtgtt tggatatagg tggaaggctt gtaaagatag ataaaataag
    32161 taaaaaaata acttatatat cacctaaaat agataaaatc ataaaagata ataatttaaa
    32221 tcttcaagta ggtacaatgg ctactgtaga aaatacaaag ccaatagtgg atataatggt
    32281 tgacttatta atggaaagca taggactaaa gaaacagaca cctttgtttg attttataat
    32341 aaccaataaa tcaataagta tagatgaaaa tattaaaaat gtttcattct ctggtggagt
    32401 agctgattat atttactatg atggagaaat aaaagattat tttgaatatg gtgacttggg
    32461 aattttactt gggcaagcaa taaaaaattg tgaatacctt aaaaaagtaa atgtagttaa
    32521 atctatggaa acaataagag ccacagtggt tggagcaggt tctcatacta ctgaagtaag
    32581 tggaagtaca ataacttaca ctagcgaaaa gtttccactt aagaacttgc cagttttaaa
    32641 attatcaatt gatgaagaaa caggtacttc aaaacaaatc agtgaagcta taaaaaataa
    32701 acttaattgg tttaaactag aaaatgattt acagaaagtt gctatagcca tgataggaaa
    32761 gaaaaatcca acctttttag atatacaaga gtatgctaaa gggtttgtag atggaatgca
    32821 acaaatcata gaaaatgatt taccttttat agtaattgtt gaaaatgata tggcaaaagt
    32881 gttaggtcaa gcaatatatg cattattaaa ttatgaaaaa gaaattgttt gtatagacag
    32941 tgtcaaggtt gaaaatggag actatatcga cattggaaaa cctatagttg aaggtaaagt
    33001 tttacctgtg gtaataaaaa cattagtttt taattaatag aaggaggtaa gtaaatgatt
    33061 ttaaatacaa aattatttgg acgtacttat cagtttaaag gcttatatga tgtaatggct
    33121 aaagctaatg aagaaaaatc aggagataaa ttagcaggtc ttgcagcaga atcagctgaa
    33181 gaaagaatag cagcaaaagt tgttttatca cacataaaat tggaggatat aagaaataat
    33241 cctgcagtgg catatgaaga agatgaagta acaagaataa tacaagatgc tgtaaatgaa
    33301 aaagtttatg aagaaataaa aaattggaca gtgtctgaac ttagagaatg gctattggat
    33361 tcaaatacta caagtcttga tataagaaga atatcaagag gattaacttc tgaaatgata
    33421 gcagcagttg caaaattaat gtcaaatatg gacttagttt atgcagctaa aaagataaag
    33481 gttactgctc actgtaatac aactatagga gaacaaggaa cattatcagc aagacttcaa
    33541 cctaatcatc caacagacga cccagatgga atacttgcat cacttcttga aggtttaact
    33601 tttggtatag gagatgcagt acttggatta aatccagttg atgactctgt tgagagtgtt
    33661 actaaagtat taaaaagatt tgaagaagta aaacaaaaat atcaaatacc aactcaaaca
    33721 tgtgtattgg cacacgttac tacacaaatg gaagcaataa gaaatggtgc tcctgcagat
    33781 ttaatattcc aatctatagc aggttctgaa aaaggaaatg aagcatttgg atttaatggc
    33841 gacactatag aagaagctag acagttagcc ttaaaacacg gtacagctac aggacctaac
    33901 gttatgtatt ttgaaactgg acaaggttca gaattatctt cagaagcaca tcatggaata
    33961 gaccaagtta ctatggaagc tagatgctat ggatttgcta aaaagtatga accattctta
    34021 gtaaatacag ttgttgggtt tataggacct gagtacttat atgatgctag agaagttgta
    34081 agagcaggac ttgaagacca ctttatgggt aaactacatg gcttaccaat gggtgttgat
    34141 gtttgttaca ctaaccatat gaaggctgac caaaatgata tggaaaactt agccatacta
    34201 ttaacaacag caggatgtac gtattttatg ggaatacctc atggagatga tgttatgctt
    34261 aactatcaaa caacaggata ccatgaaaca gcatcactta gagaaatgtt tggactaaca
    34321 gcaataaaag aatttgatgc ttggatggaa aagatgggat tctctaaaga tggtaagtta
    34381 acagagttgg cgggagacgc atctgtatta ttaggatagg aggtatttaa tatgaatgag
    34441 aaggatttaa aagcattggt agaacaatta gttggacaaa tgcttggaga attagatact
    34501 aatgttgtgt ctgagactgt taaaaaggca actgaagttg tagtagacaa caatgcttgt
    34561 atagatgata taacagaagt tgatataaga aagcaattac tagtaaaaaa tcctaaggat
    34621 gcagaagctt atttagatat gaaagcaaaa acacctgcaa gattaggtat aggaagagct
    34681 ggtactagat ataaaacaga aacagtttta agatttagag cagaccatgc tgctgcacaa
    34741 gatgctgtat tctcttatgt agatgaagaa tttataaaag aaaataatat gtttgcagtg
    34801 gaaactttat gtaaagataa agatgaatac ttaacaagac cagatttggg aagaaaattt
    34861 tctccagaaa ctataaataa tataaaatct aaatttggta caaatcaaaa ggttttaata
    34921 ttagttggag atgggcttag ctcagctgca atagaggcaa atttaaaaga ctgtgtgcca
    34981 gctataaaac agggtctaaa aatgtatggt atagattcaa gtgaaatttt atttgttaaa
    35041 cattgtagag ttggtgctat ggaccactta ggtgaagaat taggttgtga agttatatgt
    35101 atgttggttg gagaaagacc aggattagtt actgctgaat caatgtcagc atatatagct
    35161 tataagccat atataggaat ggcagaagca aaaagaacag ttatatcaaa cattcacaaa
    35221 ggtggaacaa ctgcagttga agcaggtgct catatagctg aacttataaa aactatgtta
    35281 gataaaaaag cttcaggaat agaccttaaa taaagtcgag ggagtgtaga atatgaaaaa
    35341 tgatgtaatt cgtcctaata tactaggaat taaaataatt tcaaatataa gtccagaaat
    35401 ggcacaaaaa ctagaattaa aatcacatca caaaagctta ggtttaataa ctgcagactg
    35461 tgatgatgtt acgtatacag ctcttgacga agcaactaaa gcttcagaag ttgatgttgt
    35521 atatgcaaaa tcaatgtatg caggtgctgc aaatgcttca acaaaacttg ctggtgaagt
    35581 attaggaata atagcaggtc caagcccagc agaagtaaga agtggtctaa atgcagtagt
    35641 tgattttcta gagtatggag caacttttat aagtgcaaat gatgatgatt caatagcata
    35701 ctatgctcac tgcgtatcaa gaacaggtac atacctttca gaggtagcag gaataagaga
    35761 aggtgaagca ttagcttatt tagtagcacc accacttgaa gcaatgtatg cactagatgc
    35821 agcaatgaaa gctgcagatg ttaagatgtg tgaattattt gcacctccaa ctgaaacaaa
    35881 ctttggtgga gcattactta caggttctca atcggcttgt aaagcagcat gtgatgcatt
    35941 tgcagaagca gtaaaatcag ttgcagacaa tccaacagga ttttaaggat agttagatgg
    36001 atgctatagg cttaatagaa gtaataggat atgttgctgc aatagaagct tctgatgctt
    36061 gtttaaaatc tgcaaatgtt aatattgtaa gagttgataa ggttggggca ggtattgtta
    36121 ctttaacaat atgtggagat gtcggtgctg taaaagctgc tttagaagcg ggtgaaatag
    36181 cagcttccag agttggtaca ttaagaacat cacatattat accgagaatc cataatgaag
    36241 taacaaatgc tttattcaaa actaaagaac ctaaagtctg tgaagtttca gaagataaag
    36301 aaaaaaactt tgagattaca ttggaaatag ctgataatgt atcgaaaaat ttatcagaaa
    36361 aagaaaatgt aattttagat gtcaatgaaa acagtggaga tactgaaaat ttatatagtg
    36421 atatagataa aacaaacaaa aaaattgaaa cagttgatga tagtttaaca gaagctaatg
    36481 aggaaaaaat agaaaacact aatgataaag aagtattgga acaagttaaa atagaaaatt
    36541 ccgattttga tagagatgac ctatcaaaga acagtgtaaa aaaacttaaa gctatggcta
    36601 aaaaattaga ttcttcaata acttataaag acttaaatgt tcttaaaaaa gaagaattaa
    36661 taaacttagt taaaaagctt agcagaaggg atagatagtt atgacacttt tagataaaga
    36721 tttagtatct atacaagaag taagaactct tgtaagaaga tctaaagtgg ctcaagagaa
    36781 actttctaaa atgagtcaag agcaaataga taaaatagta aaagctgttg tagatgcagc
    36841 atttaataat gctactaaat tagctaaaat ggcaaatgaa gaaactggat ttggaaaatg
    36901 ggaagataaa gtaattaaaa atacttttgc atctatggga gtttatgaaa ctttaaagaa
    36961 cacaaaaact attggaataa ttggtgaaga taaagaagcc aatacgatag atatagctac
    37021 accagttgga gttgttgcag gtcttatacc ttcaacaaac ccaacatcaa cagtaatata
    37081 caaaactata atatctctaa aagcaggaaa tactatagtt ttctctccac atccaaatgc
    37141 aaaaaactgc atacttgaaa cagttaatat aataagtgag gctgctataa aggctggagc
    37201 tccagaggat tcaatattat gtataactaa tccaagtata gttggaacca atgaattgat
    37261 gaaacataaa gatacaaact taatactagc aactggagga gaagcaatgg ttagagctgc
    37321 ttactcttca ggaacaccag ctattggagt tggaccagga aatggaccag catttataga
    37381 taaatcagca aatgttaaat tagcaatcaa aagaataata gattctaaaa cttttgataa
    37441 tggtgttata tgtgcatcag aacaatctat agtaatagaa aaatctatgg aagaaactgt
    37501 tttaaaagag cttaaaaatc aaggtgcata tgttttaaat gaagaggaaa gtgaaagatt
    37561 agcaaaattc atattaagag ctaatggtac tatgaatcca caaatagttg gtaaaagtgt
    37621 tgaggtaata gcaaatttag cagacttaac aaatgtacct aaaaatacaa aagttctagt
    37681 tgcaagagaa aataaagttg gtcacaatgt agcctattca agagaaaaac ttacaccaat
    37741 actagcgttc tatgttgaag aaaatgtaga agctattatg aacagatgca gagaaatatt
    37801 gttaaatgaa ggtgcaggtc atacattctc aatgcatgta aatgatgaag aattggtaaa
    37861 aagatttgcc cttaatatgc cagtatcaag aatacttgtg aatactcctt cagcacttgg
    37921 aggaataggt gcatcaacaa accttgtacc agcactaaca cttggttgtg gtgcaatagg
    37981 tggaagttca acttcaaaca atataggacc acttgattta ataaatgtta aaagagtagc
    38041 ttatggaatc aaagaaatag aagaacttgg aagagataaa tctcaagttg aaaaatcaat
    38101 gtttgactca atggataaag aagaattaat aaatcttctt gttcaaaaac tagtcaaaga
    38161 actagcataa ctaaaataaa caatgaaact tatggctatc gctataaatt tctaaaaata
    38221 taaactataa aaaactataa aattaaattt ttggaggata agattatggc aagtgcaaac
    38281 gcattaggaa tgatagaaac taaaggatta gtaggagcaa tagaagcagc agatgcaatg
    38341 gttaaagctg caaatgtaca attagtagga aaagagcaag taggtggagg attagttact
    38401 gtaatggtaa gaggagatgt tggagctgtt aaagctgcta cagatgcagg agctacagct
    38461 gccgagagag ttggagaact aatatcagtt catgttatac caagaccaca ctttgaagta
    38521 gatgctatat taccaaaggt atcagctgag taatataaga ataattataa aatttaaatt
    38581 ttgattgaaa aagaggctat ctcaaaaatg agtcttacta ataaactcaa tgtattttga
    38641 ggtagtcttt tttatacatc taataggaga tgtgataaaa tgggtgtttt aacagaaaac
    38701 gaagtaaaaa gaaaaattgg aaaaaacaaa aataatataa ctgaatttta tatagaaaaa
    38761 ggtcaaataa taactccttc tgcaaaatct tatttagctg agagaagtat tgagcttaaa
    38821 tatgttgata aactttcaga tgtaaacaat gaacctaaag aagttgtaaa agaagtagtt
    38881 aaagagaaaa aatataaata tactactgta tttggcgcca aattggatga aaaaccagaa
    38941 catatgactc aattaaatgg aaatctttta gtttttaaag accataaaag aataatattg
    39001 agaggaaaaa tagattctct tgaatcagaa atactaaagg tacagatact ttgtcagaaa
    39061 ctagatatgc caaagcttgt taaggaattg caagaagtat tagactttgt aagaaatata
    39121 atgagatgtg aagttttaga tgaaaagcta ggggacttta aacttttagg attaactcct
    39181 caagaactta gagaacaatc tcactatcct aaaaaatact ttggtatagg acatgagttc
    39241 ccaagttatg aaatgggaga agtagtagta gctataaata gtataagaag taatacacga
    39301 gaggttgaac ttgcagccta tgaagcattt aagggtgagt atggaaatgt tcacagagaa
    39361 gatttaataa aggcacttaa tagattatcg tcagtatttt ggataatgat atataaggtt
    39421 agaactaata agtataagta gttggaggac ataatggaga atctattaaa tgaaatagtg
    39481 gaagaagtaa taactagagt taaaaaagaa gcatttatag aagttgaagc atcaggaaga
    39541 cacgttcact taagtagaga agatgtcgat aaattatttg gagaaggata tactcttaca
    39601 aaactaaaag acctatctca accaggtcaa tatgcatgta aggaaagagt tacaataact
    39661 ggtcctaaag gaagtataaa aaatgtagtt gtacttggac catgtagaaa tgaaactcag
    39721 gtagaaatat ctttaacaga tggttcaact cttggactaa aagctcctat aaaacaaagt
    39781 ggagacttag aaggtacttt aagtattaaa atatcaacac aatatggtga agttgaactt
    39841 gataaggggc taatggttgc taaaagacat atacacatga ctcctaaaga tgctgaaaaa
    39901 ttcaatgtat gtgataaaga gatagttgaa gctaaggtga tgggtcaaag acctttaaca
    39961 tttgatgatg tagtaataag agtgagtgac agttttaaaa cttatatgca tatagactat
    40021 gatgaagcca atgcatgtgg ttattcaaaa ggaactgttg ctaaaataat aaagaaagca
    40081 taaatttaac ctactatata aattacctct agggaggatt taatatgaat tatgataacc
    40141 ttgtagattt aataacggaa gaaatctata aaaaaataaa tagtaatgaa ataaaaatat
    40201 ctaataaacc taaagctgtg attgtatttg aacaagataa caataaattt aacttattaa
    40261 aagatggatt tgaaatagta gaatttgata aaagtataag ggaatgtgaa atagtgatag
    40321 tttcaagact ttgtatgagg gggctatcga atatagcact tggaaattca acaagtgatg
    40381 aagaaagatt tatactaaaa atgattatga atggcaaaaa agtatatgtc ctagatgaag
    40441 gaattgaata caaaaaatat aaggatactg caccaaaagc actatataag aaatttatgt
    40501 catttgaaga tgaaatatgt aaatttggtg tagaaattat aaaagactta aatgttataa
    40561 ctaaaaataa gttaaatata aagaaagaac tagaaagtac tagaggttct aaaaaagaag
    40621 atacaaaagt tttagataaa gatattttaa atttaaccag tgtagaaagt gtaaaaatag
    40681 ataatgaatt tagtttggat ttaaggaata aaaaacttat atcggaagct gacttaagaa
    40741 aaccaactat aaatggagtt aaaaacatat tagttaataa aaaaagtata ataacaccat
    40801 tagctgtaga ttttatgaga atacaccatc tcaaactaaa gaaactgtaa ggaggtaatt
    40861 ttatggatat aggaaaagtt gtaggtaatg tttgggctac aagaaaagat gaaaaattat
    40921 gtggccaaaa acttttagtt gtaaaaattt taaaaactaa agacacatac aaggaaggct
    40981 tcatggttgc agccgataat gcaggagcag gaaatggaga cttagttctt atatccaaag
    41041 gttcctcagc tagagagtcg attgaaaaat ctcatgttcc agtagatgca actatagttg
    41101 gagtagtcga ttcgtttgag gtgtttgatg aataaaagta taggagctat agagtttaaa
    41161 agcattgcta aaggtataga agtatccaat gaaatgataa agaaatcttc agttgatgta
    41221 ttgtacttaa aaagtatttg cccaggtaaa tttctaataa tagtaggtgg agaaacttct
    41281 tatataaatg aatgtgtaga ttatggaata aaacttggag aaggatatat tgtagataac
    41341 ttcgtaataa atgcaatttc acaagaaata ttagatggat ttaaaaataa gtatcaaaag
    41401 ctagatagta tagtctctat aggcgttgtt gaaagtagta aaatatgcgc tggaataaaa
    41461 atgcttgata agactttaaa atctggcgac ttagtattag taaaacttca attatctttt
    41521 gctataggtg gcaaattagt atatatagta gcaggagatt taagtagttt agaatatgca
    41581 ttaaaagaaa gtgaaaatgt agtaagagaa aaagaagtaa tatataagac tgtaatacca
    41641 tcagtagaca gtcaaataat aaaaagtcta ataaaataat ggggggaatt atgagtataa
    41701 acgaaattat aatctatgta atggtagtct tcatggtact tggagctata gataaatgta
    41761 taggtaacaa atttggtctt ggagaacaat ttgaggaagg tataatggca atggggtcac
    41821 ttgcagttgc aatggttggt gttatatgtt tagcaccagt tcttgctgat gtattaagac
    41881 caattgtagt accagtattt aatatacttg gagctgaccc agctatgttt gctggaagtt
    41941 tattagctaa tgatatgggt ggtgcgccac ttgcacttga acttgcgaaa gaccctaatg
    42001 ctgggttatt tggtggatta atagtagggg ctatgatggg acctacaata gtatttataa
    42061 taccagttgc gcttggaata attgaaaaag aagaccaaaa attcttagca acaggaatac
    42121 ttgcaggtat aataacaata cctattggag cattcgttgg tggattagtt gcaggattcg
    42181 aggtaatgat ggttcttaga aacttagtgc caataataat atttgcagta gttatagcac
    42241 ttggattatt aaaattcgaa aatgctatga taaaaggatt cacatacttt ggaaaaggtg
    42301 tagtaatagt tataacttta ggacttgctg ctgccatagt tgaagcttta acaggaatag
    42361 ttgtaatacc tggaatgaaa ccaatcagtg aaggtataga aatagttgga gatatagcaa
    42421 tagttttagc aggagcattc ccattagtgt ttgttataac aaaggtattt aataaaccat
    42481 taatggcttt aggaaaagtt cttggaatga atgatgtatc agcagcaggc cttgttgcaa
    42541 gtttagctaa ctgtatacca atgtttggta tgatgaaaga tatggacaat agaggaaaaa
    42601 taataaatgt agcattctct gtatcagcag cattcgtatt tggagaccac ttaggattta
    42661 cggcaggatt taattctaat atgataacac caatgatagt tgctaaatta gttggtggta
    42721 taacagcagt attgttagct atggttatcg caaataagac actaaagaag gagaatgtat
    42781 aagatggata tatcaaatat agataaaaat ttaattgaaa cattagtaag acaaataata
    42841 gaagaaaaaa taagtggaac taaagacaca gtagattttg ttagaaataa agacattagt
    42901 ggaataactt ctataaagtt accaactgta aaagttagtg aatctgatag attagataca
    42961 gggaatccaa gtgatgtagt ttacacaaaa gatttattta cattagagga aagcccaaga
    43021 ttaggttgtg gtatgatgga aatgaaagaa acaacttttg attggactct aaattatgat
    43081 gaaatagatt atgtaataga tggaacttta gatataataa tagatggaag aaaggtttct
    43141 gcttcttcag gtgaattaat attcatacca aaaggaagta agatacaatt ttctgtacct
    43201 gattatgcta gatttatata tgtaacatat ccggcagatt gggcatctca aaattaataa
    43261 aaatcaagtt aagttaataa tcaaataaat aatttaaaat acaaactctt aaataagcac
    43321 gcatatttaa aaaagctatg aattaaaatt catagctttt tttataaaga atgttatatt
    43381 ttagacgtat tttttctata tttactggga gatatattat attttttatt aaataatcta
    43441 ataaaatgct ctacatttgg ataaccaata ctattttact gataatcttt tccttatcac
    43501 taatcttaca ttttaaaata ttatttttac tcgtaatatt atcataagaa tttgtttctt
    43561 atttaaaact aattagaatt ataaagttta ttgccatgtt attaataaaa taaatccagt
    43621 taaatcgagt gctagttaaa gtaataagaa acttttacaa tggtttcctt tatcgattga
    43681 aataaaagtt aatatggtat aatatctaaa gagagaatct tatcaattag aatatgcata
    43741 gaaacaagtg gcttaaaagg agacgttata tggaagagat agtaaagaag gaacaagcaa
    43801 gtatgaatga tatttttttg tatcttaaag ctaaatcaaa ctcattatat aaatttgtaa
    43861 tattatataa tgagtttatc aataaaccta aagattatgg aactggggaa cttataaaca
    43921 tggttgaagt acatatgcta acagcgattg aagaaaatcc aggaataagt ccaactcagt
    43981 tagctaaaat atggaacaga actaaaggtg caatatctca aactatatct aaattggaga
    44041 aaaagggtta tataactaaa gaaaagaaag aaggaaatgc aaaaactata ttactatatc
    44101 caaccacatc aggagtagag ttaagtaagg cacataaact atatgacacc atagatgtta
    44161 caagaacatt tgaatactta atcaaggagt gtacattaga ggagatagat agtttttata
    44221 aagtaattga tttgtttatt aatttattag aagaatctcc gcaggaaaat aatgattctg
    44281 aaaaagtgta gctgagattg aagttgaata aagagaaata gatattatag aagtagtact
    44341 taagtacaca ttatataaaa atataatagg tataggatta aaaagtttag tggtttaaag
    44401 tttaaccact aaactttttt aatgtctata atctactatt atttaattta ctaaaaataa
    44461 aaaaactcat tatattaaga tagaaataga aaaataacta gaaaaatact ataatatatc
    44521 agcttaatac agaaaaaagt taaaaaattt gagaaaaata aaaatgaaaa cgttttatat
    44581 tgacatagag aaaaagtagt gataccattt aaatatagtt tagtagctaa actatattta
    44641 aattataata actattttag ggggatgtta tgaatttaaa acaattaagt gaaaaatacg
    44701 aagaatatgt aattcaaatg agaagagaat ttcataagta tccagaatta tcttttaaag
    44761 aaataagaac aacccaaaga ataagggaag aattacaaaa aataggggtc ccttttgtat
    44821 ctattgaacc aaatatcgta atagctacta tacaaggcaa aaatgaaggt aagacaattg
    44881 caattagagg agatatagat gcgcttccaa tgcaagaaga ttcagatgtt gaatacaaat
    44941 cagaatatga tggaataatg cattcttgtg gacatgatgc tcatggagct atgttactag
    45001 gacttgcaca tatgctaaat gatataagaa aagatttaaa aggtagagta ttattagttt
    45061 ttcaagcagc tgaagaagtt ggtggtggac acaaggagat aatacagtac tttaaggaaa
    45121 atggaggacc ggatagatta atagctactc atgtttggtc cgatattgaa gctggtaaaa
    45181 tttctgttga accaggacct cgtatggctg gtgggtctgg ttggagaata gacataacag
    45241 gtagaggagg tcatggttct agaccagatc aaagcataga tcctataaag ccagcttgtg
    45301 atatagtttt aaaagtatct tctataccag ttaatcatat aagtgtacta gaacaatgtg
    45361 ttgtacatgc atgtgcaata catggaggaa ctacaatagg aaatgttatt ccaaacaatg
    45421 cagaggtaat aggtggatat agatatttta ctgaagaatc aggcaaaaaa gtgtttgaca
    45481 ttataaagca aatgagtaat ggtgttggac aagtttatgg agtggatgtg aaggttactc
    45541 atactggagg cataggacct gttatcaatg atgaagaagc agttaagatg gctaaagaaa
    45601 tagtttctaa tatagatgga ttggagttag attcttatga gccaatatgt gcatcagatt
    45661 gttatggtga aatattaaaa gagtattctg gcttttattg ttatctaggg gtaggtaaca
    45721 aagaaaaagg aataatatat gcacaacacc atcctaagta caatatagat gaagatatat
    45781 taaagttagg ttgcgaattt atggctaaat atgcagttga atttttaaat aaaaataata
    45841 actaaaataa atctaatttt ataaatgggg ggatgaaaat gcaaggtgca ttaaaggaaa
    45901 ataagaaaaa aagtggtgga ggatttttag gatggattga aaaagttggg aataaaatgc
    45961 cacatcctct agcattattt acatatatta cactgatagt agtagcatta tctgcaatag
    46021 caagcttttt aggtttttca gcaacaagtc ctgctacagg aaagttaata aaagttatga
    46081 acttaataag tgcaaaaggt ttagtactat ttatgcaaga gtttgttaag aacttccaaa
    46141 acttccctgt gcttggagta gttttagtta tggcagttgc cacaggagtt tgtgaaaaga
    46201 caggattttt ttcaactgcc ataaaaatga gtttatcaaa agttaagggg aatgcggttg
    46261 tatttataat agcttttata ggagtatttg ctaatcaagc tggtgatgct gcttttgtat
    46321 tagtaccaac tcttgcagga gcaatatttt atggattaaa tagaaatcca ttagctggta
    46381 tattctgtgg atttgcatca gttggtggtg gatttgcaac agctgtaatt cctgggggat
    46441 gggacgttac gctaacgcca ataactgtat cagctgctca aactattcaa cctgcatttg
    46501 atatgacatt attagctagt tattatgcac tatttgtatc tgcgtttata gtagctacag
    46561 tatcaacaat agtaacggtt aaatttatag aacctaaatt aggtaagtat actggtaaac
    46621 cagaaggtat agatgataat caaggttttg aggtcacaaa agaacaagca aaagcagcaa
    46681 agttagcagg ttttacagtt ttagcttatg tagcgttatt aattgctttg tgtataccag
    46741 ctaatagttt tttaagaagt ccagaaggtt ctttaatatt tggagcacca cttatgtctt
    46801 gcttacagtt ttttatagta atactattct tcttgccagg gattgtgtat ggaatgaggg
    46861 ttggtaaaat caagactgta aaagacttaa atgatatatt atgtcaatca atggcagcaa
    46921 tggcaccgtt tatcgtactt gcaatagtta taggtcaatt cttaacgcta ttctcagtgt
    46981 ctaatttagc acaggtacta gctataaaag gtggagcatt actcaactca ttgccactac
    47041 caccacaagc tataatactg ctattcttag tactagtagc gtttataaat atatttgtta
    47101 taagcggaag tacaaaatgg atgatatttg gaccagtatt tgttccaatg ttaatgcaat
    47161 taaacataca tccagcattt actcagtttg tatatagatt aggagattct gtaaccaatc
    47221 atttatcacc attaaatgca ttctttataa tacttttagc aacagtccaa aaatacgata
    47281 aaaatgcagg tatgggaact atattctcag ctatgatacc ttacactata ggttactttg
    47341 tagtacttgc agttttagta gtagtttgga tgacaatagg tataccagta ggaataggtg
    47401 gacaagtttg gttataaaac atatttaata attttgggag gaaaaaaaat ggaaacaact
    47461 gaaataagat acaattttga tgaagtaata gatagaagtg gaacaaactg tcttaaatat
    47521 gatcttaaga gtcaatttat gccagaatct ccagaagatg cactaccact atgggtagca
    47581 gatatggatt ttgcatgtgc ccctgagata attaatgcaa tgcatgaaag aatagacaga
    47641 aaaatatttg gatatagctc tcaacaaact gatgattatt atgaagctgt atgtggatgg
    47701 tttaagagaa gatttgattg gaaaatagaa aaagagaata tattctttag cccaggggtt
    47761 gtgcctgcgt taggattttt cacacaaata ttaacacaaa agggtgatgg tatcataata
    47821 gaaaagcctg tatactatcc ttttgaagat aaaataacta acaatgaaag aaaagtagtt
    47881 aatagtcctt taaagtttga agatggtcat tattctatgg attatgatgg atttgaagaa
    47941 ttagctaaag atgaaaataa taaggtgttt atattatgta gtcctcataa tccagttggt
    48001 agagtatgga aaaaagaaga gcttagaaaa atagtagaca tatgtaagaa atatgatttg
    48061 tggataatat ctgatgaaat acattgtgat attattcgta aaggtgaaaa gcatatacca
    48121 ttagaagtag catgtcctga ttataaagat agaataataa cttgtacagc tccaagtaag
    48181 agttttaact tagctggtat gcaattatca aatatagtaa tcaataataa ggaattacaa
    48241 gaaaaatgga tgcttgaaac agattctaaa ttagctatat tcctacctaa tccattcgca
    48301 atagttgcta ctaaagttgc ttataatgat tgtgaagatt ggatgaatca agtaaatgag
    48361 tatatagatg gtaatattaa atatgtagaa gattttgtta atgagtatat gcctaaagta
    48421 aagataataa aatctcaagg aacttactta gtttggttag actttagagg gtatggatta
    48481 gatgcaaaac agcttgaata tgtaatgctt aacaaggcga aggtattgct agatgaagga
    48541 tacatattcg gagaagaagg agcaggattt gaaagaataa atgttgcttc acctagaaca
    48601 attatagaag agtgtatgaa tagaataaaa tgtgcatttg aaggattata atagtaatat
    48661 ctaaataata tatttgtgta ttttaaaaaa agactatgct aaaaaaatat ttcaaagcat
    48721 agtcttttaa agttttatga tgagtttaaa aaatttcaaa acgttactat tcacttatat
    48781 gtttatatta gaaataattc cacaatatac tcattttcta aagttgcact tttatacacc
    48841 ttgcttcacc aatatcatgt gtaaccaaaa tagtagactt attctcattc ttaataatac
    48901 tataaacatc atcacaaact aataatctag tctgataatc aagagctgaa aaaggttcat
    48961 ctaaaagtag aatatctgga tttacagaaa gtgttctaat aagcgcaact ctttgtctca
    49021 tacctccaga taattctttt ggatacatac ttctaaaatc ccataatcca taagtcttta
    49081 aaaggctttc aactctatct aaagattttt tatctttttt gccttgaatt tccaatccaa
    49141 tagttatatt atccattata tttctccatt caagcaaatt atctttttga aacatatatc
    49201 caatatttcc agtaatcttt atatcaccac tagttggttt taaaagatta gtaagaatat
    49261 ttaatattgt agatttacca cttccagaag gaccaagcaa agttaatatt tctccttctt
    49321 tcaagttaaa attgacatcc tttaatactt caagctcgcc ttccttagtg tagaagttca
    49381 tatttatatt tttaacttct actatgttgt cattcattat gccaccccct ataactttta
    49441 gctcgtatat attatgttat taacaatatg acctatttgt accatttttc ataaatataa
    49501 tataaatatt ttgtttattt acgtattttc ttagtaactt gtacgtataa attatagata
    49561 aataaattat aaggtagggg ataaatttga gtaacataat aacgaattta atagagtcaa
    49621 tatttactaa tccaataact atattgtttt tacttgcaat aactgtgtat tctgttttta
    49681 aaataataga aaataacaga gtatattcat ttgtaagttc aaaattagac gaaatcaata
    49741 gagactataa gacaagtgag ttttataaaa aggtaaggga tgactactat acatatagta
    49801 aagaaaatcc ttatgcagac gtaaatataa cttcatttat agaggaagtt gtatctgatt
    49861 taaagcataa taatttgcca ttgttagaaa aaattagaag tataaaaaat tcatcttcaa
    49921 taagtatatt acttggggtt ttaggtacgt ttgttggtct ttctacaatg cttttatgtg
    49981 ttgatactaa agacattata aattcacttc cttcaactat tagtagtatg caaactgcat
    50041 ttacaactag tatatttgga gttgtatttt ctctaattat aggttatttt actaagataa
    50101 aagattgtga acatgttttg atacaaataa tgttaaaatc tgaaaattta ctaacatctg
    50161 aaataactca ttttaagtca gaaagaatgg atttaaaagt agaagaggta aaaaatacaa
    50221 taaaacaaat aagtaagtct atagaagcta ttgaaaaatt tgataaaata tctaaagatt
    50281 taaatgactt taatgatgaa tttatcagtg gtatagaagc tttaaaaagc cttttagaag
    50341 gttctcaaag ttctattaag acatttgacc agagcgtaag aaaattagat aaacagttta
    50401 atattttaaa tataaaattt gtaaagcttt ttgataaata tgataatcaa gataatataa
    50461 ataaagagat tttatttgat ataaaagaat cttcaaaaaa tatatacaat gccactgaaa
    50521 gccaattcaa aattagagat tatataaaaa atataaatgc aggatttgct ctatatgaaa
    50581 gaagtgctca agatttatta actaaattaa tgacccatga aaataaaatc tctcaaaatc
    50641 aaaaaatcct tttagatgaa aagtttacct tagatgactc aataaaaaat ctctcaagta
    50701 ttattgaaaa cttttctaat gacctacaag ttaaactgga tatgatgttt gaaaattctt
    50761 tagatattca agataagtta gatgttatgt ttaataactc ttttatgaat gatgaagttc
    50821 ctcttgatag tgaagaatta tttaaggatg atgttaataa tgtatttaat ccttttagtg
    50881 aagaaattta tgagatagag gataaagaaa taaaggtcat aggtgaggat gagttaaatg
    50941 aataataaat atagaagaac tgttaacaga gaatttgaaa agagtagctt ttggccaaca
    51001 tttacagatt tattatctac tgtattgatg gttgtaattc ttatcttatt tagctcagaa
    51061 agtatttcag gttctgttga acaagattta gctaagaatg tcaatgcttc tgttgaagaa
    51121 acttttaaaa aaagtggaat acctgtgaag gtagataaat ctaatgggca agtaactttt
    51181 ggtgaaaaaa ccatgtttga tgtagatagt gatgttttaa aaccagaggc taaagaaatg
    51241 ctaaaaatgt ttgttccaaa gtacatagag actatttata aagattatgg tgactatata
    51301 tccaaaatag ttatagaagg tcatacagat gatgtgggaa gttatatata caacttagat
    51361 ttatcgcaaa gaagagcata tagcgttgct aaatttatag tgggagatga aataggtgac
    51421 tataaataca aagataaagt gactaagcat attattgcaa taggaaggtc aaaagctgaa
    51481 ctcatcaaaa atggtgacaa tagcgtgaat agagatgcat ctagaagagt agaactaaaa
    51541 tacgaaataa atattaatca aaataaataa ttaaattttt ataaaaaaaa agaaggcagt
    51601 tggtgtgagc tttaagcctt ctttaaggaa aaagtttatt tatttttgtt gatacttaac
    51661 atattatagt ttttagaagc agtttgcaat atattaaacg taaatggtcg tttaaaatgc
    51721 ataactggat actactgatg taaaatattt cattttatat a
//
LOCUS       DAMKMI010000010        49980 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMKMI010000010 DAMKMI010000000
VERSION     DAMKMI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49980
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.010"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            64..1758
                     /locus_tag="PC429_000588"
     CDS             64..1758
                     /locus_tag="PC429_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF3846106.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2090..2200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2304..2675
                     /locus_tag="PC429_000589"
     CDS             2304..2675
                     /locus_tag="PC429_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF3846107.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2772..3719
                     /gene="trxB"
                     /locus_tag="PC429_000590"
     CDS             2772..3719
                     /gene="trxB"
                     /locus_tag="PC429_000590"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF3846108.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3823..4140
                     /locus_tag="PC429_000591"
     CDS             3823..4140
                     /locus_tag="PC429_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF3846109.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            4409..5695
                     /locus_tag="PC429_000592"
     CDS             4409..5695
                     /locus_tag="PC429_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF3846110.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            5807..6280
                     /locus_tag="PC429_000593"
     CDS             5807..6280
                     /locus_tag="PC429_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5939..5941,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF3846111.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            6312..7622
                     /gene="grdB"
                     /locus_tag="PC429_000594"
     CDS             6312..7622
                     /gene="grdB"
                     /locus_tag="PC429_000594"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7359..7361,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF3846112.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            7878..9410
                     /locus_tag="PC429_000595"
     CDS             7878..9410
                     /locus_tag="PC429_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF3846113.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            9421..10566
                     /locus_tag="PC429_000596"
     CDS             9421..10566
                     /locus_tag="PC429_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF3846114.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            10876..11955
                     /locus_tag="PC429_000597"
     CDS             10876..11955
                     /locus_tag="PC429_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3846115.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            12068..12667
                     /locus_tag="PC429_000598"
     CDS             12068..12667
                     /locus_tag="PC429_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846116.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(12854..13726)
                     /locus_tag="PC429_000599"
     CDS             complement(12854..13726)
                     /locus_tag="PC429_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3846117.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            14143..15546
                     /locus_tag="PC429_000600"
     CDS             14143..15546
                     /locus_tag="PC429_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846118.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            15573..17117
                     /locus_tag="PC429_000601"
     CDS             15573..17117
                     /locus_tag="PC429_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF3846119.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKSGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            17148..18539
                     /locus_tag="PC429_000602"
     CDS             17148..18539
                     /locus_tag="PC429_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF3846120.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            18732..20228
                     /gene="abfD"
                     /locus_tag="PC429_000603"
     CDS             18732..20228
                     /gene="abfD"
                     /locus_tag="PC429_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF3846121.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            20363..20638
                     /locus_tag="PC429_000604"
     CDS             20363..20638
                     /locus_tag="PC429_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846122.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            20662..21969
                     /locus_tag="PC429_000605"
     CDS             20662..21969
                     /locus_tag="PC429_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF3846123.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            21989..23107
                     /locus_tag="PC429_000606"
     CDS             21989..23107
                     /locus_tag="PC429_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF3846124.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            23394..24314
                     /locus_tag="PC429_000607"
     CDS             23394..24314
                     /locus_tag="PC429_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF3846125.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            24429..25586
                     /locus_tag="PC429_000608"
     CDS             24429..25586
                     /locus_tag="PC429_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF3846126.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(25667..26563)
                     /locus_tag="PC429_000609"
     CDS             complement(25667..26563)
                     /locus_tag="PC429_000609"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF3846127.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLISVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAKDCILHTDQGSQYTSRSY
                     SKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKEMISLINDYIYF
                     YNNERIQLKNGMSPIEYRTHSE"
     gene            complement(26521..26838)
                     /locus_tag="PC429_000610"
     CDS             complement(26521..26838)
                     /locus_tag="PC429_000610"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3846128.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            complement(26975..28474)
                     /locus_tag="PC429_000611"
     CDS             complement(26975..28474)
                     /locus_tag="PC429_000611"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF3846129.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            29070..30494
                     /locus_tag="PC429_000612"
     CDS             29070..30494
                     /locus_tag="PC429_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF3846130.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            30529..32658
                     /locus_tag="PC429_000613"
     CDS             30529..32658
                     /locus_tag="PC429_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF3846131.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFSLASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIIK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKGKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDE"
     gene            32688..33110
                     /locus_tag="PC429_000614"
     CDS             32688..33110
                     /locus_tag="PC429_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846132.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAK"
     gene            33129..34280
                     /locus_tag="PC429_000615"
     CDS             33129..34280
                     /locus_tag="PC429_000615"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF3846133.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      34386..34487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            34670..35242
                     /locus_tag="PC429_000616"
     CDS             34670..35242
                     /locus_tag="PC429_000616"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF3846134.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            35306..35956
                     /gene="fsa"
                     /locus_tag="PC429_000617"
     CDS             35306..35956
                     /gene="fsa"
                     /locus_tag="PC429_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF3846135.1"
                     /translation="MIFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHEGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            36108..39032
                     /locus_tag="PC429_000618"
     CDS             36108..39032
                     /locus_tag="PC429_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3846136.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPVLNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            39609..40067
                     /locus_tag="PC429_000619"
     CDS             39609..40067
                     /locus_tag="PC429_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846137.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            40128..40403
                     /locus_tag="PC429_000620"
     CDS             40128..40403
                     /locus_tag="PC429_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846138.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            40462..41736
                     /locus_tag="PC429_000621"
     CDS             40462..41736
                     /locus_tag="PC429_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF3846139.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            41866..42918
                     /locus_tag="PC429_000622"
     CDS             41866..42918
                     /locus_tag="PC429_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF3846140.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            42918..43949
                     /locus_tag="PC429_000623"
     CDS             42918..43949
                     /locus_tag="PC429_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF3846141.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            44041..44853
                     /locus_tag="PC429_000624"
     CDS             44041..44853
                     /locus_tag="PC429_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF3846142.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            44856..45776
                     /locus_tag="PC429_000625"
     CDS             44856..45776
                     /locus_tag="PC429_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF3846143.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            45951..46397
                     /gene="rpiB"
                     /locus_tag="PC429_000626"
     CDS             45951..46397
                     /gene="rpiB"
                     /locus_tag="PC429_000626"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF3846144.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            46427..47092
                     /locus_tag="PC429_000627"
     CDS             46427..47092
                     /locus_tag="PC429_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF3846145.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            47202..48704
                     /locus_tag="PC429_000628"
     CDS             47202..48704
                     /locus_tag="PC429_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF3846146.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(49016..>49980)
                     /locus_tag="PC429_000629"
     CDS             complement(49016..>49980)
                     /locus_tag="PC429_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3846147.1"
                     /translation="QYLVLIFFMYIYYAEAIAIVILFTLVNTVFHFYIYNFLSVKFNY
                     INNISKNIKNIENGNLKYKLEVIGDDEISTLAKSINNITGGLETAVDANIKNERMKAE
                     LITNVSHDLKTPLTSILSYVDMLKNNDFDRETINDYINILDKKSQRLKLLIDDIFEAS
                     KLSSGDVEFNITKTDIRELLIQSIVEFEDKIQNSSLDFIIETPEYAVFTMIDGKKTWR
                     VFENLICNILKYSMPNTRVYIDMFIKEENIILTFKNISNDKLNLKPEELIERFRRGDI
                     SRKTDGSGLGLSIAQNIIELENAHMEIIIDADLFKVMLTFRKVN"
BASE COUNT        19702 a         5571 c         9241 g        15466 t
ORIGIN      
        1 catagtacat gtaaatctat atttataaat acaataaagt aaagttgtaa aggagtgctt
       61 taaatgaagt tttcagagtt taagtatgaa agacccaatt ataatagtat gaaaaaagag
      121 tttttgagtt gtgtagaaga tataaataat tctcgtaatt ataaagagca acaaaaaaat
      181 atacataaga ttaatttatt aagaaataaa atagaaacgt tgtcaaatat agcttcgata
      241 agatatagta cagacacatt taataaattc tacaaagaag aaaaaaatta ttgggatgaa
      301 tatatgcctt tgtatgaaga attaaattca tatttttata atgccattgt aaattcaaaa
      361 tttaaatatg atttgataaa agagtttgga gagcaatttt tcacaatagt agagtattct
      421 ttaaaaagtt tttccaaaga aataataagt gaattgcagg aagaaaataa attgtgttca
      481 gagtatacta gacttttagc ttcagctgag ataatgtttg atgggaaaat aagaaattta
      541 tctggtatgg ggaaattcat gtattctaag aatagaaaaa caagagaatt agcaaacaaa
      601 gcatattata atttctttga agaaaatgaa actaaatttg atgacatatt tgataaattg
      661 gttaaactta gagataagat gtctaaaaaa ttaggttttg aagattttgt agagcttggg
      721 tatgttagaa tgatgagaag tgactacaga gaatatatga taaaaaatgt tagaaaacaa
      781 gtactaaaat atgttgttcc aatggcaaat gagctttatg aaaaacaagc taaaagaatt
      841 ggtctagaat atttaagtta catagatgaa ggcgtggaat ttttaacagg aaatgcaagt
      901 ttaaagggag attcaagata cataattaaa aatggtaaaa ggatgtattc tgaactgtca
      961 aaagaaacaa atgaattttt tgattttatg ttagaaaatg aacttatgga tttagaaacg
     1021 aagaaaggaa aaggagctgg agggtattgt acttatatac cagattataa gtcaccattc
     1081 attttttcta attttaatca aactgctgat gatatagatg tattaacaca tgaggcagga
     1141 catgcatttc agttgtatat gtctagatgg attgatatgc cagaaattaa ttttccaacg
     1201 cttgatagtt gtgaaatcca ttcaatgagt atggaattta taacatggcc gtggatggat
     1261 ttatttttta aagaagatac agataagtac aaattcactc acttatcatc ttcaattaaa
     1321 tttatacctt atggagtaat tgttgatgaa tttcaacact atatttacaa aaatcctaat
     1381 gtagataagt caaaacgaaa agaaatctgg agatttttag agaaaaaata cttacctcat
     1441 agaaaatatg gtgataattc ttttttagag agaggttgtt ggtggtttaa acaaggacat
     1501 atatttaaaa atccgtttta ttatatagat tatgtattag ctcaaatttg tgcgttacaa
     1561 ttttggaaaa aaatgattca agatagagat gcaggatgga aagattatat aaatatctgt
     1621 aaagttggag gaacaaaatc atttttagat attgtgagta tgggaaattt gtattctcca
     1681 tttgatgatg gctgtattga gtctattata ggtgatgtta aatcttggtt tgatgaaata
     1741 aatgacagca aattataaaa aattatgaga attttattgt ataaaagtga aaaattaaaa
     1801 aatatataaa aagtgattat ttgttgtgaa aatagtcttg tttttaacta aattgataaa
     1861 ataaactaaa aaagatttgt acttaaaaaa aaaagaaaat acaagaaata tgcaatatca
     1921 acatttggaa aaaaaagtaa aaatgtatac aaaatacata taaaaaaact attgcaaatt
     1981 ttatagaatg tttatatact taagtcaaca gagatagaaa aaagctattt atagaaaaca
     2041 aaggataaat tgcttaattt taaattttct gaaaaaaata ataatccgga tgaagagtat
     2101 gggagactgc tttataaaaa gcgaccgaag ggacaagttt aatcttactt acccaaaaga
     2161 ttagatgaaa ttctcaggta aaggtgactg tacttggacg gggctctgaa aatgatttta
     2221 acagggaaga caaattggga gatttgtttt tcctgttttt tatttatcaa gtaataaata
     2281 aaagtttgaa agagagtaaa aaaatgataa taattacgaa taatccaaaa gtaaaagaag
     2341 aagttcaagg cagagaagtt ttgtttaaag acacaactta tattggaatc ttagaagcaa
     2401 gtagagattt gatacatgaa ggttatgaac ttttatctca tccactctat gggagtgtaa
     2461 agccaaatga aacaccttac aggactgtta tactaaagaa aggaaatcgt ttggatataa
     2521 actctttaac cttaattgaa gaagcaatca tcactgcaag taaatttcag aataataaaa
     2581 agactccaaa atggacagaa agtgttcaag atgattttag agtaattgat tatgatatat
     2641 tttataatac aatacaaaga atgcagtatg aataaaaaaa aattgttaaa aagttattaa
     2701 aacagcctaa atattaaatc taatttaata ttaaataaaa tgagaaattt aattggggag
     2761 gataagtcaa aatggaaaat gtgtatgact tagtaataat tggttcagga ccagcagggc
     2821 ttgcagcagg tctttatgga gcaagagcaa agttaaaaac tctaatccta gaaaaagaca
     2881 agactggtgg acaaattgta ataacacatg aaattgcaaa ttatccaggg tcagttccaa
     2941 atgcaacagg accaagttta atagctagaa tggttgaaca gtgtaaagag tttggagcag
     3001 aaatgctaag agacaacatt gttgatactg agctagatgg agatataaaa gttttaaaag
     3061 gggaaaaggc agaatataga gcaaaagcag taataatagg aacaggagca acaccaagaa
     3121 agattggttg tcctggagaa aaagaactta ctggtaaagg ggtttcatat tgtgcaactt
     3181 gtgatgcaga tttctttgaa gattttgaag tatttgttgt aggtggagga gacagtgcat
     3241 tagaagaagc aatgtattta actaaatttg ctagaaaagt aacaattgta catagaagac
     3301 aaggatttag atgtgctaag agtgttgaag aaaaagcaaa agctaatcca aagatagagt
     3361 ttttattaga tacagtaatt gaagaaatca aaggtgatgg aatattagaa tcagttgtat
     3421 ttaaaaataa agttactgga gaaactcatg aatactttgc agatgaagaa gatggaacaa
     3481 tgggagtatt tgtgttcgtt ggattagatg ctcaaactga tttattcaaa ggtaaagttg
     3541 atatggatga aaaaggttac ataataactg atgaagatat gagaactaac ataccaggcg
     3601 tatttgcagc aggagattgt agatctaaga ctctaagaca ggttgtaact gcaactaatg
     3661 atggagcaat tgcatctata gtagctgaga aatacattga tgaaaaattt gggaactaga
     3721 atctgtaaat taagaaatag tgtctgtaaa tttaatgaat aatatttaaa attaaaatta
     3781 atatttaaaa ttaaaataaa aatttcaagg gggaataaga aaatgttaga tttagataag
     3841 gctacatttg aagaagaagt tttaaatgca gaaggttttg tatttgttga tttttggagt
     3901 gaaggttgtg agccttgtaa agctttaatg ccagatgttc ataaattagc tgagacttat
     3961 ggggataaaa taaaattctg taagatggat acaacaaaag ctagaagatt agctataaaa
     4021 caaaaagtat taggtcttcc aactatggct atatacaaag atggagaaaa agtagacgaa
     4081 gttactaagg atgatgctac agttcctaat atagaaaaca tgattaaaaa atatctttaa
     4141 tatgttgata aattattaac attaaaagaa aaaatctatt tttatctgaa gataagaaga
     4201 aaaaggtttt tatgtccaac tttgttaaaa aaataatttt aaataaaata atttttttac
     4261 aataaatatt ttttagtaat aaaataaaga tttaaggcat aaaaacacaa taatatatct
     4321 ttaaaaaaat aagataaatt gtaaaatgat ataattttga gaaaaactca aatgcatata
     4381 cacaaattaa ataacaggag gtgcaagtat gcgtcttgaa ttagggaaaa tctttataaa
     4441 agatattcaa tttggtgatg taacagaagt aaaagatgga gtattattca tcaataagca
     4501 agagatgcta caagaaattg gtggagatga gcatattaaa tctatagata tagaattagc
     4561 tcgtccaggt gaaagcgtaa gaataacacc tgtaaaagat gttattgagc caagagtaaa
     4621 agttgaaggt aatgggggaa tcttccctgg tatcatgtct aaagttgaca ctgtaggaga
     4681 aggaaaaact catgctttaa aaggtgtagc tgttgtaact actggaaaaa tagtaggttt
     4741 ccaagaaggt ataatagaca tgactggaga aggagctaag tacactccat tttctaaatt
     4801 aaataatgta gtagtaatag ctgaacctat tgatggttta aaacaatacg ctcatgaaaa
     4861 agctgttaga atgataggat tcaaagcagc aatgtactta ggagaagtag caagaaatct
     4921 tactccagat gaagtttcag tatatgaaac taaacctctt cttgaatcaa taaaagaata
     4981 tccagagctt ccaaaagtag gatatgttta catgcttcag actcaaggtt tattacatga
     5041 tacttatgta tatggagtag atgcaaaaca aatagtacca actttattat atccaacaga
     5101 attaatggac ggagcaattg taagtggtaa ctgtgtttct gcatgtgaca aaaacccaag
     5161 ttatgttcat ataaacaatg gggttgttga agacttatat gcaagacatg gtaaagatat
     5221 aaactttgta ggtgtaatta taactaatga aaatgtttat ctagcagata aagaaagatc
     5281 ttcaaactgg acttctaaat tatgtaaata tttaggatta gatgcagtta tagtttcaca
     5341 agaaggtttt ggaaatcctg atactgacct tataatgaac tgtaagaaga tagaaatgca
     5401 aggtgttaaa acagttatag ttacagatga atatgctgga cgtgatggag gttctcaatc
     5461 acttgcagat gcagatgtaa gagctgatgc tgttgtaaca ggtggaaatg ccaaccaagt
     5521 tgtagttctt cctaagttag ataaagttat tggacattta gaagtagtag atgtaatagc
     5581 tggtggttct gatggaagtt taagagctga tggaactatt gaagttgaaa tccaagctat
     5641 aacaggtgca actaatgaaa caggatttgg tcatttaact gctaaaggat attaagaagt
     5701 attttattaa acgattgagt aatgttaata aaacatatta tagataatta taataagtgt
     5761 tatagatata taaaaatata ttagaaatat aataaggagg attattatga gtttacttag
     5821 taataaaaag gttcttataa taggtgaccg tgatggtata ccaggacctg cgatagaaga
     5881 atgtgtaaaa acagtagaag gagcagaggt tgttttctca tctacagaat gctttgtctg
     5941 aacagctgct ggggctatgg acttagaaaa tcaaaacaga gttaaagatg ctgctgataa
     6001 attcggagct gaaaatgttg tgattttact aggtgctgct gaagccgaag ctgcaggtct
     6061 tgcagccgaa acagtaactg ctggagatcc aactttcgct ggaccacttg ctggagttgc
     6121 cttaggatta agtgtttacc acgttgttga ggaaccaata aaatcattat ttgatgaaag
     6181 tgtatatgaa gaccaaataa gtatgatgga aatggtttta gaagttgaag aaatagaaga
     6241 agaaatgtct ggtataagag aagaattttg taaattttaa tgcctaaaag caaaaggggt
     6301 gaaactaaga gatgggaaaa ctaaaagcag tacattatat taatcagttc tttgctggta
     6361 taggtggaga agaaaaagct gatactaagc cacatgtggc agaaacttta cctccaataa
     6421 gtttacaact tgataaatta ttaggtgaag atattgaaat agttggaaca gtagtttgtg
     6481 gagatagcta tttcaatgaa aatatagata gtgcaagtga agaagtttta tcaatggtaa
     6541 aaggttttga accacaactt ttcatagctg gtcctgcatt taatgctggt agatatgggg
     6601 ttgctgctgg aacaataact aaagttgtaa aagatgcttt gaatatacca gctttaacag
     6661 gtatgtatgt tgaaaatcca ggagcagata tgttcaaaaa ggatgtttat gtagtagaaa
     6721 cttctgattc tgcagcaggt atgagaaagg cacttcctaa gatagctaaa cttgcagtaa
     6781 aacttgctaa tggagaagaa attggaactc ctaaagatga aggatacata gctagaggaa
     6841 taagagttaa ctacttccat gaagacagag gttctaagag agctgttgac atgttagtta
     6901 agaaaataaa aggtgaacca tttgaaactg aatacccaat gccaaacttt gatagagttg
     6961 acccaagtaa agctgtaaaa gatttatcaa aatgtaaaat agctttagtt acttctggtg
     7021 gtatagttcc aaaaggaaat ccagatagaa tagagtcttc ttcagcttct aaatatggaa
     7081 cttattctat agcaggagtt atggatttaa cagaagagac ttatgaaact gcacatggtg
     7141 gatatgaccc agtgtatgca aatttagatg cagatagagt attacctgtt gatgttctta
     7201 gagatttaga aaaagaaggt gtaattggaa aattacatga gactttctat acaactgtag
     7261 gtaatggtac atctgttgca aactctaaaa aatatgcttc tgaaattgga gcagcattag
     7321 tggctgatgg tgtagatgca gtaatattaa cttctacatg aggtacttgt actcgttgcg
     7381 gtgcaacgat ggtaaaagaa atagaaaaaa ctggacttcc agtagttcat atgtgtacag
     7441 tagtacctat ttcattaaca gtaggggcta atagaatagt tccaactata gcaatacctc
     7501 atccacttgg aaatccagcg cttgacccta cagaagaaaa agccctaaga agaggccttg
     7561 tagaaaaagc attaaatgct ttaactacag aagtagatgg tcaaacagta tttgaaaaat
     7621 aaaaataaca cacacatata ttatatagat aaaaatataa acacacacta aaaataaaaa
     7681 ataactatac acaaaaaaat aatatagatt tcatgaatgg ataaattgga gttattcatt
     7741 catgaaatct taaaaaatgc caaaatacat aaacaataat agaatcaaat atacaataaa
     7801 atacaatgat tagagctttt aataagttta ataagattaa aaaataagtg ttcataaaat
     7861 ttatggaggt gcacaaaatg acttatccag ttttaaaagg ggcaggctat gtacttattc
     7921 atactccaga tatgatagtg caaaatggaa gtacttgtac agttgaaaga gcaacaaatc
     7981 cagattcaga atttttaaaa gaagtaagta atcatataag aagttatgaa gatgtagtta
     8041 actacatgcc aaatcaagtt tatataggaa atagaagacc agaagaatta agagatttac
     8101 caatgccatg gtgtgaacaa aagattgagg gaactagaaa tggtaagttt ggagaaataa
     8161 tgcctcaaga tgagttcata gctttaatgc aaataagtga tgcatttgat ttagtaaaat
     8221 tatcacaaga atttattgat gaagtaaagc caaaaatcga aaataactac cctgaaattg
     8281 caccatttgt aggtaaatta aagggtgatg atatagaaga aggtaaagaa ttagtagcaa
     8341 cacacatagc tgaaggatta taccatgatg gaaaatttgt tggttatgta aaaagagcac
     8401 atgatgtaga tgttaactta aatgcccata caatgtttga aaatctagta gtaaaagcat
     8461 ctggagtttt atctgctatt caaatgctaa gacatagcaa aatagaccct gctgaaatag
     8521 attatgttat tgagtgttca gaagaagctt gtggagatat aaaccaaaga ggtggaggaa
     8581 actttgctaa atcaatagct gagatagcag gacttcaaaa tgcaactggt tctgacacaa
     8641 gaggattttg tgcagcacca actcatgcat taattcaagc agcagcttta gtaaaagctg
     8701 gcatacataa aaatgtaatg gtagtagcag gtggagcaag tgctaaactt ggaatgaatg
     8761 ctaaagacca tgttaaaaaa ggtcttcctg tacttgaaga tgttgttgga ggatttgcag
     8821 tacttgtatc tgaaaatgat ggtgtaaatc cagttataag aactgattta acaggtaagc
     8881 atactgttgg aactggttct tcaccacaag cagttatgac agcacttata acttcaggtc
     8941 ttgatagagc taatttaaaa ataacagatg ttgatgtgta ttcagttgaa atgcaaaacc
     9001 cagatataac taagccagca ggagcaggag atgttccaga agcaaactac aaaatgatag
     9061 gggcacttgc tgttaaacgt ggagacttag aaaagaaaga attgaaagat tttgtttcta
     9121 acaaaggatt accaggatgg gcaccaactc aaggacatat accatcagga gctccttata
     9181 taggattttt aatagatgat ttaactacag gaaatagaaa tagagctatg atagttggaa
     9241 aaggtagttt attcttagga agaatgacta acttatttga tggagtatct tttatagctg
     9301 aaagaaatac aggtgttact gaagagactt caggaatatc taaggatgaa attaagaaga
     9361 taatagctga atctatgaaa aaactagcat tagatatgtt agaagaatag gggtgaggat
     9421 atgtcaaaaa aagtaatcgc agatgtattc ttagaagtag ctaatgcaat agaaagcggc
     9481 gaatttggca aaaaagtaaa aattggtgtt actacacttg gaagtgaaca tggtgtagaa
     9541 aacatggtta atggtgcaca attagcaaaa tctaatttat ttgatattgt gcttattgga
     9601 ccaaaagtgg aaactgacct tgaagtagtt gaagttaatg atgaaaaaga aatgcatgca
     9661 aaaatggaag aattattaga ttcaggatat attgatgctt gtgttactat gcattataat
     9721 tttccaatag gtgtatctac agtaggtaga gtaataactc cagcaaaagg aaaagaaatg
     9781 atacttgcaa caacaacagg aacaagtgct actaatagaa tagaggcaat ggtaagaaat
     9841 gccatttatg gtattgctac agctaaatca atgggtaaca aatgcccaaa agttggaata
     9901 cttaatgttg atggagcaag acaagttgag aagtgcttaa aagaattaaa agataatgga
     9961 tatgacatgg agtttgcgga ttcaattaga gcagatggtg gatgtgtaat gagaggtaat
    10021 gatttacttg taggtgcacc tgatgttatg gttactgata cattatctgg aaatatattt
    10081 atgaaagtat tttcttcata tactacaggt ggagattatg aagctcaagg ctttggatac
    10141 ggacctggag ttggagagga ctatgataga aaggtactta tagtatcaag agcatcaggt
    10201 tctccggtag tagcaaatgc tttaaaatac gcttatgatg ttgtaaaggg agatataagt
    10261 aatgttgcaa gaaatgaatt tgcaaaggtt aaaaaagcaa aatttgatga cattatatcc
    10321 tctcttacta aaaaagaagt taaagctgaa aaagtagaag ttaaaatgcc agataaagaa
    10381 attgttactc gtcaaatagc tggtgttgat ataatggatt tagaagatgc agtatctgag
    10441 ttatggaaaa acggaatcta tgctgaaagt ggtatgggtt gtacaggtcc aatagtttta
    10501 gtaaatgaag ctaaaggaga cttagcagtt gaaactttag ttaaagctgg atatactgca
    10561 aaataagtac attttttata tgtaaataag aacatatagt tataatttta ttgattataa
    10621 ttatatgttc tttttatttt atatcttgtc aatatttata tattaacaca atccatttat
    10681 ttgattctct agctaattta ttatatttat tcaattaaaa ttaagctttc taactaaata
    10741 aaaactaatg aagtattaaa atgaaattga taaaatatga aaaaatttta ggaataaagt
    10801 atctgttaga taaaaataag attaattagt gatataatat aattaagata aaatttatta
    10861 tttggaggta aaatcatgag cagagtaaaa aatgtagtag aattgttgga aactaaaggt
    10921 gttgatgcac tatatctaac aaagaaaact aatgtaaact atataagtgg ttttcctgat
    10981 gaagaagcat atgcagtgat ttgcaaagat ggaaactttt tagttactga tagtagatat
    11041 atggagcttg cagaaaaggt ttgcaaagat tttgaaatta taaattggca taattttgat
    11101 agaagtgtgg caaaggcagt taaaagtgta tgtgataaag tgggtataaa gaaacttgga
    11161 tttgaaagaa ctaatatagt gtttgataaa tatgaagaac ttaaaaatct gattgagaag
    11221 gataatggag aattaatacc tacagaaaat attgtagaga ctttgagata tgtaaaagac
    11281 aaagatgaaa tcaaaaatac aagaaaagct tgtgaaatag cggataaggc attagaagaa
    11341 ttgattccac atataaaagc tggagttagc gaaattgaac tggctacaag gttagagtac
    11401 tttatgaaaa tgaacggagc acaaaatata gggtttgaaa ctatactgat atcaggagca
    11461 aagacttcat tgcttcatgg taagcctagt gataagatta tagaaaaagg tgattttgta
    11521 cttatagact atggtgctat gtataatggc tatatatctg atacaactag aacttttata
    11581 gttggaggag cttctgaaaa gcaattagaa atatataatt tagttaagga agctcaaaat
    11641 gtaggggttg agaacatgaa agctggtgta catgcgacaa ttccagatgc tgagataaga
    11701 aaagttgtca aaaaatatga agactattac tatcaaggta taggacatgg agtcggtaga
    11761 gatgttcatg aagaaccttt cattggtaat tatggtgata aaatcataga agagggatgt
    11821 ataataacaa tggaaccagg tatttatttc cctggatggg gtggagttag aatagaagat
    11881 actgttttga ttactaaaaa tggtccagaa agacttacta aattccctaa agatttgatg
    11941 attttagaca aataatttaa tttatatata tttttttaca taaaatataa cataatctca
    12001 ctattaagct ctatttacat attatagtaa agagggctta attttattgt gagaggtgat
    12061 agtatatatg tataaaaaac ataaatttat aaaaaatata aaaaaagcct ggtataaaat
    12121 caaattaatt gatagatgcc ttataattgt aatggcaata ttaatgtttc aatctatata
    12181 taacttattt gttaatgaag taaattcaca agatactaca actattgatg tagtagtaag
    12241 aaccacttca gcagctatat ttgggtattt tttaagtgct aattttataa agagaccttt
    12301 aaggaagtat acaaatactg atgtatcttc aaatcttttt atagataata atttatcccc
    12361 aaaaggtcaa gagtctaatc aaaataatat aatgaatgtc aaaaatacca ttggatttac
    12421 ttcagaagct gataattatg aaaagaaaat acctattaat aataatgaag gttttgaaga
    12481 aggagaaact agtgaattgc aaattattat agctactgta atttgcatag tagcattaac
    12541 agtacttttt gttgtaagaa attttacaac tacaacatca gcttctttag gtactatttc
    12601 acagatgaga gattttgtct caggatgcgt gggttttttg ctaggttgtc caagtaaacc
    12661 tgattaaaaa agtattatca ataaaaggta ttttaaaaag tatcagtata ataatattta
    12721 gatttaaatg tatacaatta taattctgta acattctata ttgtattttt atactgatac
    12781 tctaaactat actatttatt aaaatatttt aacattgtat ttaaacaaaa tattatatct
    12841 gtataatata aatttagttt atatgaatag atgccatata ctcaataaaa agtttcatac
    12901 tatgagatat gtattgattt cttctccaaa gaaccccaat atttgcagta attgcacgtt
    12961 ctaatggtat tgaacagaga ttttctgtat tttgaaatat ttctttatac aatattgttg
    13021 aagccaagtc attttctagt aaataacgta tggtagaaag ttgatttgag tgtaaaatta
    13081 tatttggctt agctttttca agtctttttg taataatctt atagtgataa gaaccttcag
    13141 ataagattac aaaaggtaca ttttttaaca tataattcga tataatagga tgttttgcca
    13201 attcattttt gggatttgta actaagtata attctgtttt atagattgtc tttgaatcta
    13261 tatcatcata aatatcactg tcaattatac caatacaaaa atcaagtgta gattcttcaa
    13321 tcattttagc tccaacaaat gttggaactt caaaaatttt aagctctata tctggataaa
    13381 ttacattaaa cctaggaagt atttttgaaa gcaaaaacgt acctaaaact gtaggaatac
    13441 ctattttgag agaagctttt ctcttttttc ctaaatcaag tgcgtcacat tgaaaatcct
    13501 caaattgttt tataaaatgt tttgacttgt ctaaaaaaat ttttccttca ggcgttaact
    13561 ctattttatt acctattctg taaaaaagtt caaagccaag ttctttttct aattctttaa
    13621 ttgctaatgt tatggatggt tgagaaatat gaatactttc cgcagcctta gtcatgtttt
    13681 tatatttaca gacagtttga aaatagtata aatagtttat attcatttgg tattagttcc
    13741 tttcatttac atattagtga caagtatata attaacaata gcataagttt tgtatattgt
    13801 tgtaaaaaaa tagaatagtt ttaaactatc atagcataag tttttgatat tttcaatcta
    13861 ttaagaatag tgctattatt aaattaacaa aagagaatag ctggtatggt ttagagtaaa
    13921 tcaatctata ttacagttat atcaacgaat atcaaaaaat tatctattaa aaaataaaaa
    13981 ttatttttac aattaaattg ttaattaaat cataaagaaa aaagtgatat taacaagata
    14041 aaattttttt aatcagaaat gttaatttaa taacaaaata gagtattggg gagaaaggag
    14101 aaatatattt tttaataata tttgttaatt taataacgaa aaatgaaagg gagtgattta
    14161 gtgaagtttg aattgatgga ttttttaatg aatccttttg tccttatgtt tgctgcagta
    14221 attacaggaa ttttatttgg aaaaattaaa tttgggaagt ttaattttgg ggtatcagga
    14281 gcattattta caggattatt tattggatgg cttgcatata gcttggggaa tctaataata
    14341 gcaaaagggg aaacagcagc aggatataag gcagcaactg taatgatggg gaatggaatt
    14401 atttcttctg acttctttga tttcttttta ataatatttg ttgcagcagt tggtctattg
    14461 gcagcaaaag atatgaaagc tgtattaaag aagtatggag caagatttgt aattttagga
    14521 gttcttataa cttttatagg tggctctatg acttatgcaa tgactttact atcaagtgat
    14581 aaaggaagta gtgcttatga agtttctggc gtttacacag gagcattaac aagttcgcct
    14641 ggattagctg ctgctttaga aacagctggt aaacatgcag aagatgtatc aaaagaattt
    14701 gaaaaagctt caataaaaga taaaaaagaa attttaaaag tagtcgaccc agaaggcaaa
    14761 ttggatgtga atacaactac atcactgact caagagcaaa tagataagta tattgcatat
    14821 gctgaagctg gtgttggtat aggtcatgca gtcgcatatc cttttggagt tttaatagta
    14881 atcttaggtg taaatttcct tccaaaatta tttaggatgg acctaaaaga agaaagaaga
    14941 aaatatgaga aggaaatgaa ggaagcaaga gatagtgttt ctggtaaaac gataccagaa
    15001 gttccattta atataataac atttttcttg acatgtttag ctggatactt agttggagga
    15061 atacatgtat ttatgggacc actagggtac ttcacactag gtgcaacagg aggttcacta
    15121 attgtatctc tagttcttgg atatataggt aagatagggg tagtaaattt cagaatggaa
    15181 gaaaaggttt taaatatttt aaaacaaata gggctagtgt ttttcttagc aatagttgga
    15241 ttaagatatg gtggaaaagt agttgattca attatgacat ctggtatgca cctagcctta
    15301 gtagcaatag cagtaggtgt tacagctatg atgataggat tcttagttgg caaatacgta
    15361 tttaaattaa attggatttt attatctgga gctgtctgtg gtggtatgac atcaacacca
    15421 ggtcttggag ctgctgttga tgctcttgat agtgatgacc cagcagcagg ttatggtgct
    15481 acgtatccat ttgcattatt aactaaagtt atattggtta tagttttaca caaacttcca
    15541 atgtaaataa gattttatat ggggggatat ttatgaataa tgaaggagca tttgaagaat
    15601 taagtggaag attaagaaat aaagaattgt gtgatttaat aatgaatgca gatgaagctg
    15661 ctaaaattat agaggatggc atggttattg gagttagtgg atttacacct tcaggctatc
    15721 caaaagcagt accattagca gtttcagaaa gagctaaaag tggagaaaag ataaaattaa
    15781 ccgtatattc aggagcatct ttaggtccag aagttgatgg agcatggtca gaagcaggta
    15841 taattgaaag aagacttcct tatcaaacta attcaatact tagaaataat attaataaag
    15901 gtgttgttga ttatatagat atgcacctaa gtcattctac tcaattttta aattatggaa
    15961 ctatacctaa agtagatgta gctatagtag aagcacttgc aataactgaa gaagggaata
    16021 ttataccaac tagtgggata ggtaattctc catcttttat aaaaagtgca gataaagtaa
    16081 tagtagagat aaatcttgca aaaccaatgg aaatggaagg tatggcagat atatatatta
    16141 cggaaaatcc accaaataga aaaccaatag aaataaatca tcctaaggat agaattggaa
    16201 caacatatat accttgtggt ttggataaaa ttgcaggagt agtaattacc aatatgcaag
    16261 ataagacccg tccattgggg gttgtagatg aagcttcgaa aaaaatatca aataatatta
    16321 ttgctttttt aagagaagaa gtaaagtcag gcagattatc aaagaattta cttcctcttc
    16381 aatctggtgt aggaagtgta gcaaatgcag tattatatgg attatgtgaa tctgaatttg
    16441 aaaatttaac ttgttataca gaggttgtac aggactctat gttggattta ataagaatgg
    16501 gtaaagttac aatggcatct actacatctg taagtccatc tccagaagga ttaataaaat
    16561 ttgaaaaaga tattgatttt tttaaggata agattatatt aagacctcaa gaaattagta
    16621 acaatccaga aatagccaga agaattggtg taattgcaat gaacactgca attgaagttg
    16681 atatttatgg aaatgtaaat tctactcaca taatggggtc aaagatgatg aatggaattg
    16741 gtggttctgg tgattttgct agaaatggag caataactat atttagtact gagtcaattg
    16801 caaaaaatgg tgatatatca tcaatagtac ctatggtatc tcacgtagac cacactgaac
    16861 atgatgtaat ggtaattgta acagaacaag gatatgctga tttaagaggt cttgcaccta
    16921 gagaaagagc tataaaaata atagaaaatt gtgcccatcc agattataaa gaacaactta
    16981 gggattattt aaatagagct tgtaaaagtg gagcaaaaca aacacctcac atattggacg
    17041 aagctttatc atggcatagt aaatttatga gtacaggtac aatgaaaaag gctgaaactt
    17101 ttaaaagtgc attataaaat atttaataaa ttggaggtag attttaaatg gaaaaagcag
    17161 tggaaaactt tgaagattta agtaaggaat acattaatgg ttatattgag agagcaagaa
    17221 aagctcaaag agagtttgaa tgttatactc aagaacaagt agataaaata gtaaaaatag
    17281 ttggaaaagt agtgtattat aatgctgaat atttagctaa acttgctgta gaagagactg
    17341 gaatgggagt atatgaagat aaggtagcta aaaataaaag caaagcaaaa gttatatata
    17401 acaatttaaa agataaaaaa tcagttggta taattgatat agatagagaa actggaataa
    17461 caaaggtagc taaaccagtt ggagtagtag cggcaataac tccttgtaca aatccaatcg
    17521 ttacacctat gagcaatgca atgtttgcac ttaagggaag aaatgcaatt attatcacac
    17581 ctcatcataa agcaattgga tgtagtacaa aaactgtaga gatgattaat gaagaattag
    17641 aaaaaatagg agctccagaa aatttaatac aaatacttga ccaacaatca agagaaaata
    17701 caagaaattt aatttcatca gcagatgtag taattgccac tggaggaatg ggaatggtaa
    17761 aagctgcgta ctcaagtgga aaaccagcac ttggtgtagg ggcaggaaat gttcaatgta
    17821 tcatagatag agatgtagac attaaagaag cagtacctaa aatcatagca ggaagaattt
    17881 ttgataatgg tatcatttgt tcaggtgaac agtcagtaat agtagcagaa gaaatgtttg
    17941 ataaaataat ggatgaattt aaaaataata aaggttttat agttagagat aaagtacaaa
    18001 aagaagcttt cagaaatgct atgtttgtaa ataaatctat gaataaagat gcagtaggtc
    18061 aatcagttca tacaattgca aaaattgcag gtgttgagat accagaagat acaaaaataa
    18121 tagtaataga agctgatgga ccaggagaag aggatattat tgcaaaagaa aaaatgtgtc
    18181 cagttatatc tgcatacaag tataaaagtt ttgaagaagg tgtagctatt gcaaaagcaa
    18241 atttaaatgt agaaggaaaa ggacatagtg tatctataca ttcaaataca gttaaaaata
    18301 tagaatatgc aggagaaaat atagaagtat caagatttgt tattaatcaa tgttgtgcta
    18361 ctagtgctgg aggaagtttc tttaatggtc ttgcacctac taatactcta ggttgtggtt
    18421 cttggggaaa taacagcatc tctgaaaatc ttgattataa gcatctaatt aatatttcta
    18481 gaattgctta ttatatgcca gaaaatgaag tccctacaga tgaagaactt tggggataaa
    18541 atttagacaa aaaatattca ttctaaatta aagtaggaat atattttaat ttagaatgaa
    18601 tttaagacat aaattaaatt taaattagtt tattggacta aatttaaaat attaagagac
    18661 atatagaaga aggaagtatt ataatttaaa ataaataaaa aaatctataa aagagaagga
    18721 gtggttttat tatggcatta atgacaggag ctcaatatat tgaaagtttg agaaaattaa
    18781 atactaaggt ttatatgttt ggtgaagagg ttaagaattg ggttgaccat cctatgatta
    18841 gaccatctat aaattgtgta gcagcaactt atgacttagc tcatgaccca gaatatgcag
    18901 atttaatgac agttacttca aatatcacag gtgaaaaaat aaatagattt ggtcatcttc
    18961 atcaaagtgt tgatgatttg ataaaaaaag taaaaatgca aagactatgt ggtcaaaaaa
    19021 cagcttcttg tttccaaaga tgtgttggta tggatgcgtt taatgcagtg tattcaacaa
    19081 catttgagtg tgataaagcc catggaacta attatcatga taattttgta aaatacttaa
    19141 cttatataca agaaaacgat ttagtagttg atggagctat gactgaccct aagggagata
    19201 gaagtctatc acctagtgct caacctgacc cagatatgtt ccttcatata gtagaaagaa
    19261 gagaagatgg gataattgta agaggagcaa aggcacatca aactggttca ataaattcac
    19321 atgagcacct tataatgcct actataagta tgactgaagc agataaagac tacgctgtat
    19381 catttgcagt tccttcagat gctgaaggag tgtttatgat ttatggaaga caatcttgtg
    19441 acactagaaa attagaagaa ggtgcagatg ttgatttagg aaataaggag tttggaggtc
    19501 aagaagcttt agtagtattt gataatgtat ttgttccaaa tgatagaatt ttcttatgtg
    19561 gagaatggga tttctctggt atgttagtag agagatttgc aggataccat agacaaagtt
    19621 atggtggatg taaagtagga gtaggtgatg taataatagg agcagcagct ttagcagctg
    19681 attataatgg agcaaacaaa gcttcacata taaaagataa actaatagaa atgactcacc
    19741 ttaatgaaag cttatattgc tgtggtatag catgttcatc agaaggtcac aaaacagaag
    19801 ctggaaacta tcaaatagat ttacttcttg caaatgtgtg taaacaaaat gtaactagat
    19861 ttccttatga aatagtaaga cttgcagaag atatagctgg agggcttatg gttactatgc
    19921 cttcagaaaa agactttaaa tcagacctta aagttggaac aagtggaatg actattggcg
    19981 aagtatgtaa caagtatttt aaagcttctt cagtggcttc aacagaagaa agaatgagaa
    20041 tattaagatt cttagaaaat atatgccttg gttcatctgc tgttggttat agaactgagt
    20101 caatgcatgg ggcaggttca ccacaagctc aaagaataat gatttcaaga caaggaaaca
    20161 taaatcaaaa gaaagaatta gctaagaaaa ttgcgggaat aaaaaaagag gaagctttaa
    20221 atttataaaa agttgatata taaagggaaa ttatatatta atgtgtatta attaatgaaa
    20281 aaatatgtag tataagagat agttataaaa ttatggtggt ttttagctaa gtaaagaaat
    20341 ttttaataag gagaatttac acatgacctg tggagttaga ttttgtgggg gctgtaaccc
    20401 tagatacgaa agagggaagg ctcttacaat aatcaaagag cacttcaatg gtagggtaga
    20461 ttttagtata gcacaagaga acgtcgagta tgatttgcta cttataattg gaggatgcgc
    20521 aactctatgc gcatcctatc aacaatataa agtgaaaaat gaatgtgtta agatgtggga
    20581 ttttagccat atagaaagag ttataaatgt gattgaatca gcaatttgtg aaacttaaaa
    20641 aagctaaatg gggggatttc attgagttgg caagaattgt atcaaagtaa attatgtagt
    20701 gctacagaag ctgtaaaaca gattaaaaac ggtgatactg tagtatttgc acattgtgta
    20761 ggtgaacctc ctgcactagt agaggctatg attgaaaatg ctgaacaata taaagatgtt
    20821 gagattaaac atatggtaag cttgggtagt ggtggatata ctgctaaggg aatggaagct
    20881 cattttagag taaatccaat gtttgtaagt ggaaatgtaa gaaaggcaat tgaaaatgga
    20941 gatggtgatt ttacacctgc attcttccat gaagtaccaa agttgcttag agaaaaaaga
    21001 ttaaaatgtg atgttgtttt agcacaggta actccaccag atgaacatgg gtattgtagc
    21061 ttaggaacat cagttgatta tacttatgaa gccattaaaa ctgctagaac agtaattgtt
    21121 caagtgaatg accagtttcc tagaacctat ggtgaggtag tccatgtatc agagtttgac
    21181 tatatagtgg aaaaatcaca acctttattt gaactacaac ctgcaaagat tggagaagta
    21241 gaagaagcaa ttggtaaaaa ttgtgcttca ttaattgaag atggttctac tttacagcta
    21301 gggattggtg ggataccaga tgcagttatg ttatttttaa ctgataaaaa ggatttaggg
    21361 attcattcag aaatgatttc agatgggact ttggcccttt atgaaaaagg tgttataaat
    21421 ggtaaatata aaaattttga taaagaaaaa atgacagtta ccttcttaat gggtactaaa
    21481 aaattatatg actttgcaaa taataaccct gcagtagagg taaaaccagt agactatgtg
    21541 aatcatccag caattataat gaaacaacat aagatggttt ctataaattc tgctatacaa
    21601 gttgatttaa tgggacaggt agttgcagag gctatgggac ttagacaatt tagtggtgtt
    21661 ggaggtcaag ttgactttat acgtggagtg tctatgggtg aagatggaaa ggcaattata
    21721 gctatgcctt caataactac gaaaaaagat ggtacagtaa tttcaaaaat cgtgtctatt
    21781 gtggatgaag gtgcacctat aacgacatca agaaatgatg ttgactatat tgtaacagaa
    21841 tacggtatag cagaattaaa aggaaaatct cttagagaaa gagcgagaaa tttaataaat
    21901 atagctcatc caagtgtaag agaaagtcta gcagtagaat ttgaaaagag atttaaggag
    21961 aaatattaat aatagagggg tgttaataat ggaagcactt agagtagttc caaagatatt
    22021 ttactttgat acatttaaag aatttaatga agaatttaaa ataggaaaaa atgatttagt
    22081 aattactaat gagtttatat atgagccata tatgaaacct cttggaatag atacaaattt
    22141 aatttttcaa gaaaagtttg gaacaggtga accatctgat gaaatgatag actccatgac
    22201 aaaggaaatg aagaaataca attttgatag aattattgca tttggaggag gaacaatagt
    22261 tgatatatgt aagatattag cacttgatgt tccagaaaag tctattgatt tatttgaagg
    22321 agaagtatca cctaaaaaag taaaagagtt agtagtagtt cctacaacat gtggtacagg
    22381 tagtgaagta acaaatgtag ctattgcaga gttaaaatct aaacatacta aaaaggggct
    22441 tgcagtagaa gaaacatatg cagactatgc agtgttaatt ccagagacag taaaagggtt
    22501 accttacaaa ttctttgtta caagttcggt agatgctctt atacatgcta ttgagtctta
    22561 tctttctcca aaagcaagtc catttacaga gatgtattct ctacaagcaa taaaaatgat
    22621 tatggatggg tataagaaga tagttgataa aggagaagaa gagagattta atcatcttag
    22681 agactttgta cttgcatcaa attatgcagg aattgctttt ggaaatgctg gttgtgcagc
    22741 agttcatgca ctatcttatt caataggtgg agctttccat gttgcacatg gtgaagcaaa
    22801 ttatcagttc tttactgaag tatttaagat gtattctaga aaaaaaccta atggaaaaat
    22861 tataaaatgt acaaaaattt tagcagatgc ccttgaatgt gaccctaact gtgatgttta
    22921 tggagaatta gaaaagtttt taaataaatt aatagctaaa aaagctttga gagaatatgg
    22981 aatggtggaa agtcagatag atgaatttac agatagtaca atagcaaatc aacaacgact
    23041 acttgctaat aattatgtag agcttagtag agaggaaata agagagatat ttgcaaatct
    23101 ttattaatac aattttttat ttatatgtat agtcaacata aacaatgcta attatattta
    23161 tgtacaaata aaaataaggg aacttaaatt ttaattaaag ttaagttctc ttatttttat
    23221 atggagttaa atataagtat atttttaatt taaaatatgc ttatatttaa gtctattact
    23281 ttatttgaaa caatttgtag aatttcaaat tccttgacag taataagtaa gtaattgtat
    23341 aatcaaactg caatatatta ataatattag tgaaaatgaa aggattataa aatatgtata
    23401 aatcagaaaa tttttcaaaa aaatctgtat taataaattc atttttaagt aaatttttaa
    23461 aaataattgg atggggtttg gtaagcttat ttacattatc agccttagta ctttcaacta
    23521 tttccattat aattggatgg gctgtaatac ttcctattca agtatgtgca gtatttttac
    23581 ttataggagg agtttgtatt tttaaaggta agagattagg agaccaaatt tctcgttata
    23641 aaaaatattg tacaattata aacaatagaa atattattcc agtagaactt attgcagaaa
    23701 acacatctaa atcattaaat tttataataa aagatgtaca aaagatgata gacaaaaact
    23761 actttataaa tacttatatt gataaaagaa ataatcagat tgtgttaaca aatgaagatt
    23821 ttgttccacc aagatatgaa gatgtagcat atgaagtcaa agaagaagaa aagactgatg
    23881 aatccaatga agttgataca attataaata aaggaatgaa ttatctaaca caaataaaag
    23941 aagctaataa aaatattaaa tcagaatcaa tgggcgataa aattgtacaa gtagaagatg
    24001 ttacaagtaa aatatttgat gttgtaaaac atgacccatc aaaacttact caaatacaaa
    24061 aatttatgga ttattatctt ccaaccactt taaaactatt aaattcatat catacattgg
    24121 aagaacaagg aattgataga gaaaatataa caacaactat ggaaagtatt gaaaatacaa
    24181 tgaatacgat tgtagtagct tttgaaaatc aattggacta tctgtttgaa gatgaagcca
    24241 ttgatatttc tacagatatt actgttttag agaatatgtt agtccaagaa ggattaacga
    24301 gtggtaaatt ttaacttcaa tagaaaaaat attctgttat ttttaaacaa ttaattagtc
    24361 ataaagtata aaatgtgtat tatataagtt aatcagtact ttatgaagga agaggagaga
    24421 aagatacaat gaataattta gatgatatac cagttatgcc aactttgaca cttgacccat
    24481 ttggagaaga atcaaacact aatgatatag ataattctga cttattaatg aaaaaggatg
    24541 aaaaagaccc agaagaagaa aaactgtctg aatctgaacg caagatggta aaggaatttg
    24601 cggaaaaaat agatattaca aatacaaata tgattttgca atatggagct ggtgctcaaa
    24661 aaaaggtagc aagtttttct gaaacagctt taaagagtgt aaagacaaga gatttaggag
    24721 aagttggaga tatgcttaca aatctagtta ctgatttaaa agcattttca gcagatgaaa
    24781 aagaacaaag tggtttttta ggaattttta aaaaggcaaa taataaaatt tcaaatttaa
    24841 aaactaagta tgatagtgca gaggtaaatg tggataaagt aagcaaagag cttcaaaagc
    24901 atcaggttaa aatattgaaa gatatagcaa tgcttgataa gatgtatgaa ttgaacttag
    24961 cttactcaaa agagcttact atgtatatta tagcaggaaa acaaaaatta aaggatatga
    25021 aagaatatga gatgccaaag ttgagagaaa aagctcgttt atctggttca acagaagatg
    25081 ctcaatctgt aaatgatatg gtttcacttt gtgacagatt tgaaaagaag attcatgact
    25141 tagaattaac tagaatggta tcgcttcaaa tggctcctca gataagattg gttcaaaata
    25201 gtaataatct gatggcagaa aagattcaat caactatagt aaataccatt ccattatgga
    25261 aaaatcaaat agtgcttgct cttggaattt ctcacttaaa tcaagctatg aaagcacagc
    25321 gtgaagttag taatatgaca aatgagcttt tattaaagaa tgcagaaact ttgaagatgg
    25381 gaacaataga aacagcaaaa gaatcagaaa gaggaattgt agatatagac acaattaaga
    25441 aaactaatca atctctaata tctactattg atgaggtagt taagatacaa catgatggta
    25501 gaataaagag acaagaagca gaagttgaat tatcaaaaat agaaaatgaa cttaaaagca
    25561 agcttcttga atttacagtt aaataaaaat tatactaaga taaaaagaga ctgtctcaaa
    25621 attggatatg tccctgtcaa gtagacagac ttaaaaaagg ctatctttac tcggaatgag
    25681 ttcgatattc aatcggactc attccgtttt ttaattgtat tctttcatta ttataaaaat
    25741 aaatataatc atttattaga ctaatcattt cctttggatc tgtagtatca attaaatata
    25801 ttaattcaga tttaaaatga ctgaaaaaac tttctatagg agcattatcc cagcagttgc
    25861 cccttcggga cattgattgt ataataccat tatccttaag ccttttagag taagatctac
    25921 ttgtatattg tgaaccttga tctgtatgta aaatacaatc tttagctaaa ttcatattta
    25981 ttagttgatt tactgtatta ttaactaatt tagtatccat ttttgtactt atctcatatg
    26041 ccactatttc accattaaat aaatctttgg ctacattcat atataagaat tttttaccga
    26101 taggtatgta tgttatatcc atacaaattt tttctaatgg ttgattagct ctaaattcac
    26161 gatttaatat attatcatat atctcatatg atttaaactt acgtctatat actttctttc
    26221 taataataga tttaatacct agttctttca tcaacctata tactctctta tgattaatag
    26281 gggtatcaag tattttattt aaataagtac aaattctttt tctaccataa gttcctctat
    26341 attttttatg tatttcaatt atataatcct taataattct atctttgata cccctatcgg
    26401 aagatttttt agaataactt agccatttat agtaaccgct tcttgacaca cttatcaact
    26461 tacacatact agaaatacta tacttcctag acagaagttt aattgtttta aatttatcgc
    26521 ttactttgac ttgtccaata gggtacttaa cttttttaag aattcattct ccatgcgtaa
    26581 gtacttcaat tcttcttcaa cagatttaaa ttttctatta tcattttcta gactaatatt
    26641 tttaaatctg cccctagttt catcatcaaa tgcagttttg cctttatttt tataatcttt
    26701 tacccagttt tgaacttgtg ttttgcttct tatattcagc atctcagcaa ttttgtatga
    26761 tgagtatcct tcttttatat ataagtttac agcttttagc ttaagttctt tgctatattt
    26821 tttgtgtttt ttagacatag aaaaaccccc ttagagtata cgctttttag acctattata
    26881 atagcctttt atttaaatgt ctactataag gggattatac taaaatagag ataagtctct
    26941 ttttcattgt ttaaatgatt aaagtatatt tatattataa atttctatta tcttttaaca
    27001 taacatcatg agctccacct tcaactatac tagtagaaga tacaagtgta agtttagcat
    27061 ttttttgaag ttcaggaatg ctaagtgctc cacagttaca catagtagaa cgaactttac
    27121 ttaaagaaag agttacattg tctttcaaag aaccagcata aggaacataa gagtcaacac
    27181 cttcttcaaa agaaagtttc ttatctccac caaggtcata tctttgccag tttctagcac
    27241 gagcagaacc ttctccccaa tactctttca tataactacc atttatatta actttgttag
    27301 ttggactctc atcaaaacga gagaagtatc taccaagcat tataaaatct gcacccatag
    27361 ctaaagctaa agttatatga tggtcataaa cgataccacc atcagaacaa attggaatat
    27421 aaatacctgt tttttcaaag tattcgtccc tagcttgagc aacttcaatt atagaagtag
    27481 cttgtccacg tcctatacct ttttgttcac gagttataca tatagaacca ccaccaatac
    27541 caactttaac gaagtcagca ccagcttcag ctaggtaaag gaatccctca cggtcaacta
    27601 catttccagc tcctacctta actgtatcac catatttttc acgaatgaaa tcaagtgtta
    27661 ttttttgcca ctcagaaaaa ccttctgaag aatctataca taaaacatca gcaccagctt
    27721 caactaaagc aggaacacgt tcagcaaagt ctctggtatt aataccagct ccaactacat
    27781 atctcttaga agaatctaaa agttcaagtg gattttcttt atgagaagag taatctttac
    27841 ggaataccat atacataaga ttaccattgt catcaagtac aggaagagaa ttaagtttgt
    27901 ggtcccatat aatgttgttt gcttccttta aagtaacatc tttgttagca taaactatag
    27961 atgacatagg agtcataaat tcactaactt tagtatctaa atccatacga cttatacgat
    28021 aatcacgact tgcaacaata cctaataatt taccagttga agttccatct tcagtaacag
    28081 ctactgtaga atgtccagtt ttttctttta agtcaagtat gtcttttaat gtgttatcag
    28141 gtttaatatt tgaatcactt acaacaaaac cagctttgtg ggatttaaca cgtgctacca
    28201 tagcagcttc atcttcaata gtttgagaac cgtatataaa tgaaatacca ccttctttag
    28261 caagtgcaat agccattttg tcatcagaaa cagattgcat aatagcagaa gtcaaaggta
    28321 tattcatata aatatctgct tcctcgccct ttttaaattt agtaacaggt gtttttaaac
    28381 ttacattagc aggtatacaa tcagtagaag agaaacctgg tacaagaagg tattcactga
    28441 aagtatgtga tggtgtatca aaataaaacg ccatttttta atcactcctt atatttttat
    28501 actttttata attgatagta ttaattataa cttattcttt aattttatta aagtattttg
    28561 tcaaatattg agatttttga aaaatttaaa ctattttaat tgaatttgtt taatatatat
    28621 tgccttatag ggtaaagttg tatattaaat aatgtttagt gtttaatagt agctaatttg
    28681 aaattaatct ttagttattc atataattac atttgtatag tttatattga taatattgct
    28741 agagagtgca taaacctcaa aaatacagat aaattaaatc tatattagta tatccaaaag
    28801 tacatttaat aaatttattt aactgaatta ttattttcat ttctattaaa tttaaatatt
    28861 ttataaaaat agtattattt acaaaatcat atactccaaa atactacata aataaaatta
    28921 aacaaaaact aaaatgacaa atgtcattat ctaaagtgac atagaaacgg tttcttaaag
    28981 ttgttactat aaaatataaa ctctataata aaagtataac aaaagggaat catatcgata
    29041 tagaatataa ttcaagagga ggtaaaatta tgagtagttc taatcaatcg acaataaaga
    29101 catctataca gcgagtaggg aaatttttaa gtggtatggt aatgccaaat ataggagctt
    29161 ttatagcatg gggtcttata acagcattat ttataccaac aggatggatg cctaatgaaa
    29221 aattgagtac tataggcgac ccaatgataa aatacctttt accactattg atagcttata
    29281 ctggtggtaa agcaatagca ggacaaagag gtggcgtaat aggtgcagca gcagctatgg
    29341 gagtaattgt tggggcagat ataccaatgt ttatcggagc aatgataatg ggaccatttg
    29401 caggatgggt aattaagaag ttcgataaat ttgtagatgg aaagatacca acaggatttg
    29461 aaatgttagt aaataacttt tcaataggta taataggtat gcttcttgca atactaggat
    29521 tttatgcaat tggaccagca atagtagcag ggacagctct tatagaatct ggagttcaat
    29581 ttatagttag caaaagttta ttgcctttag tttcagtatt tatagaacct ggaaaagtat
    29641 tattcttaaa caatgcaata aatcatggta tattagggcc tataggtata gctgaggcaa
    29701 aagaagcagg taaatctata atgttcttac tagaaagtaa ccctggacca ggtttaggag
    29761 ttctattagc atattggatg tttagtagag gaagtgtaaa acaatcagca ccaggagctg
    29821 taataataca tttctttggt ggtatccatg aaatttactt cccatatatt ttaatgaatc
    29881 cagttttaat attggctaca atggcaggtg gagcagcagg aatattgact ttttcaatat
    29941 taggagctgg gcttgtagca gcaccatcac caggaagtat atttgcactt atggcactag
    30001 ctccaaaggg aggattactt ccagtattag caggggttgc agtggcaaca gttgtatcat
    30061 tcttagtggc agcaccattt gttaagagag cttcagcaaa tgaaagtgaa gaagacagta
    30121 cttctttaga agaagctaaa gcaaaaatga gtgatatgaa atctgcaagt aagaactctg
    30181 agaaaaatat tgagaaaaaa cagcttgaag taaatgagat taagaagatt gtatttgcat
    30241 gtgatgcagg aatgggttcg agtgcaatgg gagcttctag atttaaaaat agaataaaaa
    30301 acttagattt agatatagaa attacaaatt catctgttga taatcttcca gatgatacac
    30361 aaatagtagt aactcacaat acattagttg aaagagttgc taagaacaat tcaagtgttg
    30421 agatagtttc aataaacaat tttttaaatg acccaaattt agatacatta tttaaaagat
    30481 tagaatctaa ataaaaataa aaagtaccac taattaggag tggataattt gaaaaaaaag
    30541 aagattacat ctaggcaaaa aaagataata ctaatgatag tagaaaattc aaagaaaaac
    30601 attccaataa ctatttctga gattgcaggg acgttagagt taagttcaag aactgtactt
    30661 agagatatgt ctggaattga aaaatggttt gatgaaaatg attttaactt tgtaaaaaaa
    30721 ccaggagtag gattgatttt agaggaaaat atagaaaatc aaaactttat aattgagttg
    30781 ttagaagaag aaaaaataga aaaagaatac agcaaggaag aaagaaattt aattatatta
    30841 tcaaagctac tagtttctaa cgagccagta aaatcttatt attttgcaaa aatattaaag
    30901 gtatctgaag gggtactgaa taatgatttt tcattagcaa gtaaatggtt ggaaagattt
    30961 gatattgagc ttgttagaaa accagggctt ggtgtctacc taaaaggtca agaaaaaagc
    31021 tttagagagg catatgtaaa tcttatatac gattcattca atgaaaaaga aatactagat
    31081 atggtcagaa atataagtga aaatatacaa acagataaag caatagaaat tttaagtgaa
    31141 aatagacttt taaatttaat ggataggtgc ataataataa aagttgaagc tacattaaca
    31201 aagaagcttt cagatttaga tgtaaattta gctgatagtg catatatagg gcttgtagtt
    31261 catatttcat tagccttaca gaggataaaa aatggtgaaa atataactat ggataaagag
    31321 tttctaaaag aattatctat aacagaagag tttaaattag ctaaagaaat cgtaaaaggt
    31381 atggaaacag attttaacat ggatatacca gtagatgagg ttggatatat aactatgcat
    31441 ataagaggtg caaagcaaag gtcatcttca aatcataagg ctttaaactt ggatgatata
    31501 gaaataatgg aaattacaaa taagatgata gatttggctg aagatgaatt taaaataagt
    31561 ttaaaaaatg acgagaggtt atttaaagac ctagcaaatc acttaggacc ttctataaat
    31621 agattgaata tgggattaga aataagaaat cctcttttag atgaaataaa aagcaagtat
    31681 tcatatgcct atgatggagt tgaaaaaatt tctagaatta taaaagataa actaaatata
    31741 aattcaatac ctgaatcaga aataggatat attgcaatgc actttgcatc agccatagaa
    31801 aaaaatctta tgatgaatac taatataaat attgttgtgg catgtcccac tggaataggt
    31861 acttcaagat tcttatcaac taagattgaa aataaatttc ctaatttaaa tatacttgaa
    31921 accatatcag ctataaacat agatgaagag tatttaaagg gaaaagatgt tgatttaata
    31981 gtgtctacag ttgagttaaa tacaagttta aattatatat gtgtaggtcc atttatgagt
    32041 ttagatgatg aacagataat caaggaaaaa ataaaatcta tagctcaaaa taaattaatt
    32101 aatttaaata caaaaaatga tactaaaagt aagaataaag tatatgaaca gataacagaa
    32161 agtatgaata taggaaaaga tattcttcaa tttttagagg aaattagatt tgaaaaattt
    32221 gaaagtaaag atttaagtga gcttattgaa gactcatcaa aaatatttgc aaaatctagt
    32281 gaagatataa tgtctataaa agaatcgtta aaagaaagat taaaaatctc aattccatat
    32341 atagaagaat caaaaattct attattacat tgtatgagtg aaagaataga tattatgaag
    32401 ttatcaataa taaaactgga gaatcgaatc gtgctagatt caaatgaaga aatcgacaat
    32461 gtagttttta tgttacttcc taaaaattca ccaagttatc aaagacaaat aatgagtgaa
    32521 ataagtggtt cattaataga caatataatt tttactaata agataaataa attctctata
    32581 gaagagatga cattagaggt aaaagatata gtttttaatt tttatacaaa cagattaaaa
    32641 gcatttatag atgaataaaa ttaatattaa aaaggaaggt tgatactatg agtaaagtat
    32701 tgaatgagaa taatatattt ttagggttag atagtgtttc aaaggaagaa gctataactt
    32761 tagcaggaag aaaattagtt gaaaatggat atgtaaaaga agaatatata ccagcaatgc
    32821 tagaaagaga aaaagtaatg actacatata tgggaatggg agttgctatt ccacatggtg
    32881 taaatgaagc aaaaaaagaa atattatctt ctggaatagt tatactacaa ttccctaatg
    32941 gtatagattt tgatggagaa aaagcatact tattaatagg aatagctggt gttggagatg
    33001 aacatttaga aatactatct aatatagcag tagttttaga tgatgactta acagaaagac
    33061 taaaaaattc aaatgacaaa caagctttta tggaagcttt tgccaaataa tcttaggagg
    33121 gatttaaaat gaaaaaggca atccagtttg gagcaggaaa tattggaaga ggttttattg
    33181 gaggcttgtt agttaagtcg ggttatcatg tagtatttgc tgatgtaaat gaggagatat
    33241 taaactcaat aaataaagat aaaaagtata ctatacacat aagagatgta gaatgtatag
    33301 atgaagtaat tgataatata agtgcggttt catctataaa agaagaaata atagatgaga
    33361 ttgtacaggc tgaaataata actacagcag ttggaccttt agttttgact aaaatagcct
    33421 ctacaattgc aaaaggtata aaggctagaa aagaaaaggg tttaacaagt aatctaaata
    33481 taatagcctg tgaaaatgca atatacgcaa gttcttcttt aaaagaagag gtacttaagt
    33541 atttaaataa ggaagaagta gaatatttag agatatatgt tggttttcca aactgttcag
    33601 ttgatagaat agttcctcct ggtaaaaatg aaaatcctct tgatgtaaca gttgaaaatt
    33661 tttatgagtg gaatgttgaa aaacaaggat ttaaagggga aataccaact atagttggaa
    33721 tgaatttagc tgacaacctt atggcttata tagagcgtaa attgtttaca ctgaatacag
    33781 gtcatgctat aacagcttat ataggatatt taaaaggata taagactata gaagagagta
    33841 taaaagataa atttatctgt gatatagtca aaagtgcaat ggtagaaagt ggagaaggat
    33901 tgattaaaaa atacaatttt gactcagaag cgcactataa atatattgat aaaattttaa
    33961 atagatttaa aaatccatat cttaatgatg atgtattaag agttggaagg gaaccattaa
    34021 gaaaactaag tgataaagat agattgatta aacctttaat gacagcaaaa tcatatggat
    34081 tatcagttga caatttgatt cttggaatag gtgcagcgct tcattataat aatagtgaag
    34141 atactcaaag tgtagaatta caagagttga taaagtctaa tggtgtaaaa aaagctgttg
    34201 ctaaaatagc taatataagc aatgacgaag aacttttaaa taatatcgaa aaaagttata
    34261 tatttatgaa aaatttataa atttacttga aaaaaagtag ttctgataaa gtaactataa
    34321 tatattattc tacacagttt gaattatttg ttgaataata gagaattata atatataatt
    34381 aaaaggaata aattaatata tttacctgta tatgtttgat aatatggttc aaatgtttct
    34441 actaagctac cattaatagc ttgactacag gagtgaagaa gatatagttt atacttattt
    34501 taaattatat attaagttaa ttactttgaa ttttaagctc ctgtctatga tatagacagg
    34561 agctttttta gtattttaat ataaaatact tacaagaata gatagcaaaa ttagaacaaa
    34621 tataattttt ttattaatat aactttagaa agggctaggt ggaagttcaa tgaaggcttt
    34681 acaagaaaag atattgagag aaggcagtgt ttcaggaaat gatatactaa aagttgatag
    34741 ttttttaaac catcaaattg atgtagcttt tttaaatgaa ataggaagag aatttaaaga
    34801 gagatttaaa gaagaaaagg tggataagat atttacaata gaggcttcag gaatagctat
    34861 agcatcaatt gtttcacagt attttgataa tgcacctgta gtatttgcaa aaaaatcaga
    34921 atctaaaaat ttggataaag atgtatatga gactaatgta tattcattta ctaaggcaag
    34981 agaatattca gttaaagttt ctaaaaagta cataaacaaa ggtgaaaata tattgatagt
    35041 ggatgatttt ttggcaaatg gaagagctgc tcttggactt aaagatttaa ttgagcaagc
    35101 agaagctaat ttagttggag taggaattgt cattgaaaaa ggatttcaag ctggaggagc
    35161 acttttaaag gctaatgatg taagattaga gtctttggcg gtagttgaat ctatagataa
    35221 tggaactgta aaatttaggt aatttatgat attatatact tgtgtttagc acatatttta
    35281 actatgtaat tatggaggat tttttatgat attttttata gacacagcca atattgaaga
    35341 aataaaagaa gcaaatgatt taggtgtaat atgtggtgta actactaatc catctctaat
    35401 agctaaagaa ggtagagatt ttatagaggt agttaaagaa ataagtgaaa tagtagatgg
    35461 accaataagt gcagaagtaa taagtttaga gcatgaaggt atgatagagg aagcagaaaa
    35521 attatctaag atacataaaa atatagttat aaagatacct atgacagcag aaggtctaaa
    35581 agcggtgaaa gtactatcaa gtaaaggtat aaaaactaat gtaacactta tattctctgc
    35641 tggacaagct cttttagctg ctagagctgg agctacatat gtaagtcctt ttgttggtag
    35701 attggatgat atatcacaaa atggtcttga tttaattgaa gaaatcgttg atatatttag
    35761 tgtaaatagt attgaggctc aaataatagt tgctagtgtt agaaatccaa tacatgtttt
    35821 acaagctgct agaatgggag cagatatagc aacagttcct cttaaagtta taaaccaaat
    35881 gattaagcat cctttgacag acaaaggaat agatagcttt atgaaggatt gggaaggtgc
    35941 ttctttaaaa ttataattat aaactacgct ttataagtat attaaagagg ttattatgga
    36001 aattctaagt aataaggcta ggctattttg cctagtctta ttttggtgta taatatattg
    36061 aaacatatta ttgaaataga ttaatttttt aagtgaggag gcgaaaaatg gataaaatat
    36121 taagagagat acaaagagaa gataaaaaaa atccatatac tgaccaagaa cttgctgaaa
    36181 tgttaaatgt agctaggtct gaagtgatat cagtgaggaa aaaaaataat attttagatt
    36241 cgagagaaag aagaaaaaaa atattaataa aagatatatc aaaaattgta agtgaaaatc
    36301 ctcaaatgtc tgaaagaaaa ataactgaga tattaataga gaatggatat aatatatcga
    36361 gaagtgcagt ttcaaaatta ttaaaagaag aaaatttaaa tcagtcaaag attgggatta
    36421 aagataaaga gccagcatca tcaggaaagt atgtgaaggt gagtaaagtt gtagaagaag
    36481 atggatttga agaattgata ggtataaagg gaagtttgaa agaaaaggta aatctagcta
    36541 agtcagcaat aatgtatcct cctaatggcc ttcatactat aatatatgga gaaacagggg
    36601 taggtaaaag tgaattagct acatgtatgt ataaatatgc tataaaaaac aacatcaaag
    36661 aagagaacag cccatttata gtgtttaact gtgcagacta tgcagaaaat cctaatctat
    36721 tgatagctca actctttggg gtagtaaaag gtgcatatac aggagctgat gcaaatagag
    36781 aaggtcttgt agaacaagct aataatggaa tattgttttt agatgaaatt catagattgc
    36841 ctgctgctgg gcaagaaatc ttattttcat taatagatag aggagaattt agaaggttag
    36901 gagaaagtag ttctaataga aaggcaaatg ttctcataat aagtgctaca acagaaaacc
    36961 cagattctaa tttacttcaa acatttagaa gacgtatacc aatggtaata aatttaccta
    37021 atatgagtga aagaccaaaa tctgaaagat atgaaataat aataaagttt tttgaaagag
    37081 aagcaaagcg agtaaacaag aattttataa taactaaaga agttatgtgt gcacttatga
    37141 attataagtg tactggaaat ataggtcagt taaagagtga tatacaagta acttgtgcta
    37201 gagcttttag taaatcaata ttttctagcg ataaggtaat tatagattta gatagtttaa
    37261 gggattatat aaaaagtgga tattatgatt gtaattgtaa aaatgaaaag tattctgagt
    37321 tttcagttga ggatatatat atagatatca gtgaattggt gagtggaaaa aatagaattt
    37381 tatctgataa tgagataagc gagatatata attatgcaga aaaagagctt aaggttttgg
    37441 aaagtaagtg tttttcagaa gaagagttaa aaaatgcatt tatagaaaag ttagataaga
    37501 agtttaatgc tattaaaaac aataaaaatt tatctgatag aagaagtcga atagactggg
    37561 gaggacaatt aaaagagtct acattggaga taatggataa ggtaattgta tttattaagg
    37621 agcagtttaa gcatgtaaat caaggattgt acttggctct agcaattcac attgagcatg
    37681 ctataggtag gataaaagat ggaaaaacta taataaatcc gagtttagat aaaataaaag
    37741 catcaatgcc aatggaatat gaattgtcaa gatatataat gggtatagtg gaagatttga
    37801 cacaagtaac atttccagaa gatgaacttg gatatttggc ttattatata aataaatttt
    37861 gttataatga ggaaagtata aaagataaag ttaaagtagt aatagttacg catggtaaag
    37921 ttggaattga aatgtctaaa gttgtaaatc atattttagg tatagaatgt acactaggta
    37981 tagaaatagc acttacagat tcaccatcag aggggataga gcatgtatta gaagagcttc
    38041 aaaagataga agctagaaaa ggtattctag ttctaattga tatgggttct ttggtaattc
    38101 ttggtgatga ggttgaaaaa agacttggaa ttagatgtaa aactgtaaac agagtagata
    38161 cgttattggc aatggaagca ggaaaacttg caaccattga aggaaaaagt ttagatggta
    38221 taatagcaga tttaaagaaa aataagaatt atgccatgat aaatacaaat aaatttagtt
    38281 acagaaaaaa cgagtatgga aagaaaaatg taatcattac tttatgtttg agtggtgtag
    38341 gtacagcttt aaatttaaaa gagcatatag aaaaacaaat taaggaatat gacactttaa
    38401 ttgaggtaaa gccagttgct tttttaaata ataatttaga agaagagtta aatgatattg
    38461 aagctgaata taatattatt gctatctgtg gaactatcga tatagattat aaaaacgtac
    38521 cttttatttc ttataatgag gtacttggta aaaatggtat taataaagta ttagagcatt
    38581 taaacagtaa acaaccagtt ttaaatattg aaaaccagtt gaataattta atacatgaag
    38641 atttgatttt atacaatttt gaaggaattt caaaggagta tataattgat actcttgttt
    38701 caaagcttga agaaggtgga tatgttgact caaagtatat attaagtgta tataaaagag
    38761 aagcaatggg aagtgttgta atggcctcta aggtagccgt gccacatgga cttccagaaa
    38821 acgttataaa gcctgcaata gcaatcgcaa ggttgaataa gcctatagtg tgggacaata
    38881 aatttatggt ggatttggta gtactactgg cacttaaaga aaataataag aaagaaatac
    38941 gaagcttatt ttcaaagatt aatgaccaac atactttaga agttttatta aatactgaag
    39001 ataaaaatga aataaaagaa cttttaagct aattattagt acagagaaga aagacaaaaa
    39061 atttgcttta gtataaaatt taaaactact taattgctaa gtagttaatt actcgatata
    39121 aggctagttc tatgaaattt atttatatga ttttatagaa ttagcttttt tcttggtaaa
    39181 taaaatatat gaatttattt atgcaaaaag taaattttat tggacaattc tatgagaatt
    39241 tttcctatat ttcttataaa atatagaata tataaaaata aattaaattc ataataaata
    39301 attttataaa atagttgtta aacataaaaa accatttgta aaaaacaaaa aaatcatttc
    39361 tctaacaaaa aaaaccacaa aaaatataaa aaagtatggc tagttaagtg attaacaagt
    39421 aaaaattaaa ataaaaatca aaaaaaattt atcaatgaaa gtcagctaaa atcctggaag
    39481 agagcattat ttttgggaaa acattttctt ttgtttcaca aaaaaagcca ctttttggca
    39541 cacaacatgc tttatataaa gtcaagaaaa actaaggtat aagacaaagg caattaggag
    39601 gtgtaaatat ggagtttata aattttgtaa gtgataaagt tatagacctt aaaatggatt
    39661 gtaaaacgaa agaagatttc tttaaagaaa tacataataa agtttttgat ttaggctttg
    39721 taaaagaaga atttggagaa aaaatattag caagagaaaa tgtatttcca acaggtttaa
    39781 atttaggaga ttatggagta gcaataccac atacagatgc agaatatatt aaagagcaat
    39841 ttatatcagt ctgtactttt aatgaaccag tagtgtttag ttctatggaa gaccaagatg
    39901 aaaaagtacc tgtaaatttg gctttcgtat taggacttaa tcaaccacac agtcaattat
    39961 ctgtattaac agaattaatg ggaattatgc aaaataaaga gttagttgat aagttgatga
    40021 gttctactga taaagaagaa gttttaaaga cactaaagtc tttataaaaa taaaaaagta
    40081 ctaaaaataa aaaatataaa aataaaaatt attaggagga aataaaaatg aaaaaaatat
    40141 tagtagcatg tggagcagga atagcaacat caacagtagt ttgtgacaag gtagagagat
    40201 tagttaagga aaataatata caagcagaag ttatacaatg caaaatagct gaatgtgcaa
    40261 caaagcaaga aggagcagat ttaatagtat caacaactat actaccaaca acttataata
    40321 taccagcaat aaaagcaaca gcatatataa ctggagtaaa tacagcagca ttagataaaa
    40381 aaatattaga tgctttaaaa taatcctatc atagatacat tgaaataata ttttataaaa
    40441 cagaaaaagg aggatttata aatggataaa ttattagcag tagtacaata cattttaaat
    40501 atgggaccga cagtaatact accaataaca attgtagtac taggaatgat atttagaatt
    40561 aagtttacaa aagctttaaa gtcaggtctt acaattggta taggatttgt gggtataaac
    40621 ttagttgtaa acctattaac atcaacacta gggccagcgg ctcaaggaat ggttgaaaga
    40681 ttcggactta atttaacagt aatagatgtt ggttggccaa caacagcatc agcaacatgg
    40741 gcttcaccag ttgcaccaat attaatacca gtttgtttat tagtaaactt actattatta
    40801 tacttcaata aaacaaagat attaaacgtt gatatatgga attattggca ctttatagta
    40861 gcaggtgcag ttggatatat agtaacagga agttatgttt gggctatatt ctgtgcaata
    40921 ctaatggagt tattagtact gtttattgca gaaaagacag caaaaaatgt tcaggagttt
    40981 tatgatttag aaggtatagc attaccaact ggttcaacag cttcatttgc tccacttgga
    41041 tatttagtag gtaaatttgt tgaaaaaata ccaggaataa ataaaataca agcagaccca
    41101 gactctatac aaaaaagatt tggtatattt ggagaaccaa tgatgatggg gattatacta
    41161 ggtataatac ttggagcact tgcgggatat gatgtagcag gaatattcaa tgtaggtata
    41221 tcaatgggtg cagtaatgtt acttatgcct agaatggtta gaatattaat ggaaggttta
    41281 attccaattt cagaatcaat aagagatttc ttacaagcaa aatatggaga cagagaactt
    41341 tatataggtc tggatgcagc agtagcaact ggacatccag cagttatatc aacagctctt
    41401 atacttgttc ctataacttt gttcttagca gttatactac caggaaacaa agttttacca
    41461 tttggagact tagcgacaat accattttat atggcattta tagtgtgttt tagaaaaggt
    41521 aatatagttc aatcagttat aacaggaaca atagtaatag ctatatcact ttatatggct
    41581 acaaactttg cagatgttca tacacaatta ttacaacaag caaatatgat gccagaagga
    41641 acaacaatgg ttacaagtat agatacaggt ggaaacttgc taaaatgggg aatattgaaa
    41701 ttatctcaat tagtttcttc atttggaata ttctagtatg tagttaatag agattactct
    41761 tttttagtaa agtatgcgaa aatttaacat ttagcagcta ctaaaaaaga gtaatatttt
    41821 ttataaattt ttaaaaatac aaaatacaag aggaggagat ttatcgtgaa agcagcagta
    41881 ttacatggca caaatgatat gagatttgaa gatatagaaa taaaaccatg tgaaagtgat
    41941 gaagtaaaaa taaaagttat ggcagcagga atatgtgggt cagatcctcc aagagtatta
    42001 aaacattgga aatatccagt tccagctata ccaggacatg agttttctgg agtaattgct
    42061 gaagttggta aggatgtaaa aaatgtaaaa gttggagata gagtagttgc aattcctttt
    42121 ataccttgta atgaatgtga atattgtaaa agaggattat tttcattgtg tgatgaccat
    42181 ggaatgttag gagctaaaag ttttggagct tttgcagagt atgtaaatat aaaagctaca
    42241 aatgtacttc caataggaga tatgaacttt gaagatgctg caatgataga gccactagca
    42301 gttgctatgc atggagtact taatataggt gttcaagttg gagacactgt agctgtaatg
    42361 ggtagtggaa caatggggca gttggtaata caaggactta aaatagcagg ggcaggaaca
    42421 attatagcag ttgatatatc tgataataaa ttaagagaat caaaggaatt aggggctgat
    42481 ataataatta atgctaaaga tattaaccca gtagaaaaaa taaaagaact tactggtgga
    42541 aaaggtgttg acatagctct tgaatgtgca ggttcaaaaa taactcaaga acaatgttta
    42601 cttataacta aaaagaaaag taagatagga tttttaggga tagcatattc agatataact
    42661 ttatcagaag aagcatttga aaatatattt agaaaagaac tagaattaaa aggtttttgg
    42721 aactcttatt ctgcaccatt tccaggacaa gaatggacta agggaatcaa tctagttaat
    42781 gaaggaaaga taaaattaaa agaaatggta tctcatagat tctcacttga agatacttac
    42841 aaagcatttg aaatgataag agatagaaaa gaagaattta acaaaatatt aattttacca
    42901 caaggggttg aaaaataatg aaagcagtag ttaaaactaa accaggctat gataatgtag
    42961 aagttttaga agtagaagaa ccaaaagcta caggagacaa ggttaaaata aaagtagaat
    43021 atagtggtat atgtggttca gatatacatt catttaaagg tgaatatgca aatataaaag
    43081 ccccagtaac attaggtcat gaattttctg gaattgttgt agaagtagga gaagatgtta
    43141 aaaatataaa agtaggggat agagttacat cagagactac ttttgcaact tgtgagaaat
    43201 gtatatattg tgaaactaaa gattataact tatgcccaac tagaaaaggt attggtactc
    43261 aagtaaatgg aagttttgct gagtatgtat tatcaagaga agaaagcata catgtacttc
    43321 ctgaaaatgt atctttatta tcagcagccc ttacagagcc actagcttgt tgtgttcatg
    43381 cagctcttga aaagactact atagaaagtt ctgatacagt tttaataata ggaccaggac
    43441 caattggttt attattagct caagttgtta aatctcaagg tgctacagtt ataatgtctg
    43501 gtgttacaaa agataaggaa agattagaaa tagctaaaaa tcttggagta gatagaactg
    43561 taaatgtaat ggaagaaagt ttggcagatg tagtgaatga aatgactgat ggaaatggag
    43621 taaacaaagg atttgattgt tctgggttta tgccagcagt taacgaagcc ttaagactgc
    43681 ttagaaaaaa aggtactttc gtacaagttg gaatctttgc taaaaaatta aatgaaatgg
    43741 accaagaagc aataatccaa agagagttac aatatatagg aagcaggtct caaaagccaa
    43801 gttcttggat tacagcatta gatttaatgg caaatgggaa agtaaataca gaagctttag
    43861 ttactagaac tgttggttta gacgaattta aagaaggtat tacagcttta atgaacgggg
    43921 aagaaataaa agtagcagta aaatcttaaa aaaacatttg tattatttta aaaaaaattg
    43981 taaaatctac tgtaggataa aaaaatgtaa ggtcttaaaa aataacagga ggaagaaagt
    44041 atgagcactc ttagagatca ttctaataat attagaaaaa acataataaa aatggttgca
    44101 gaagctaaaa gcggacatcc aggtggttca ttatcaatag ctgatatgtt gacagtatta
    44161 tattttgata agatgaacgt atgtgcagaa aatccaaaaa tggctgatag agatagattt
    44221 gttctatcaa aaggtcatgc agcaccagct ttatatgcta cattagcaga aaaaggattt
    44281 ttcccagaag aagaattaat aacactaaga aaatttggtt ctaagttaca aggtcaccca
    44341 gacatgaaaa aagtagcagg aatcgatatg tctactggtt ctcttggtca agggctatct
    44401 gcagcaaatg gtatggcact tgcaggaaaa ttagataaca aagactatac agtatatact
    44461 atattaggtg atggagaaat ccaagaaggt caaatatggg aagctgcaat gacagcatca
    44521 cattacaaat tagataattt aattgcattt gttgacttaa atggattaca aatagatggt
    44581 tctaatgaag aagtaatgaa tgtaagtcca gtagatgaca aattcaaatc ttttggatgg
    44641 aatgtaatag taataaatgg acattctttt gaagaaatag gaaatgcagt tgatgaagct
    44701 aaaaaagtta caggaaaacc aactgtaata atagctaaaa cagtaaaagg taaaggtgtt
    44761 tcatttatgg aaaataatgc tgcatggcat ggaacagctc caaatgcaga acaagcagag
    44821 caagcattga aagatttaga aggaggtcta taggaatggg aatagcaact agagaagctt
    44881 acggacaagt gttaaaagaa cttgcagaaa ataaagatat agtagtatta gatgcagatt
    44941 taggaaaagc tacaaaaagt atatcattta aagaagttgc cccagataga ttttttgaca
    45001 tggggatagc agaaggtgac atgattggta cagcagcagg tcttgctact tgtggaaaaa
    45061 taccttttgc gagtactttt gcaatatttg cagcaggaag aggctatgag caaataagaa
    45121 actctgtagc atatccaaat ttaaatgtaa aaatagcagc tactcatgca ggtgtaacag
    45181 taggtgaaga tggtggtagc catcaagcta tagaggatat atctctaatg agaggtatac
    45241 caaatatggt tgtattaaat ccagcagatg ctttagaagc aagacaagct attcttacat
    45301 ctattgatta taatggacct gtttatataa gattaggtag agctgcaact cctgatgtaa
    45361 acagtgaaaa ttataaattt gaaataggaa aaggaactgt tttaagagaa ggttctgaca
    45421 taacagtaat agctacagga ataatggtag caaaagcctt agaagcagca gaagaacttg
    45481 ctaaagaagg tataaatgta gaagttgtta atatatctac aataaaacca ttagatgaag
    45541 cattaataaa agaaactgct aagaaaactg gaaaagtagt tacagcagag gagcacagta
    45601 taataggtgg tttaggttca gctgtatgtg aagctttagc agaaactaaa gatgtagtag
    45661 ttagaagaat tggtgttaaa gatgtatttg gtcaatcagg tacaccagct gatttattaa
    45721 aacactatgg tttaactaca gaagatatag taaaaaatat aaaagagttg ttatagatat
    45781 aaatatatct aaataacaaa ttgatttgac ataagtcaat taaagctgtc ttaaaaatag
    45841 aaagaaattt ctacaatggg acagctttat ttttacaata ctttattttt atgataatta
    45901 taaaatagtc tgaaagtaat caaaaaaatt ataaaaagag gtgctattta atgaaaatat
    45961 ctattggatg tgaccatggt ggttatgaat taaaggaata tataaaggaa aatttaataa
    46021 ataaaggtat agaagttgaa gatgtgggta caaattcaaa agaaagtgta gattttccaa
    46081 tatatgcaaa aaaggtaagt caaaatgttc aaagtaaaaa atctgattta ggcatacttt
    46141 gttgtggaac aggaataggt atgtctattg cagcaaataa gtttaaagga atcagagcag
    46201 ctgttgtttc tgactgtttt tcagctaagg caactagaga acacaataat acaaatgtac
    46261 tttgtttagg agaaagagtt attgggaaag gattagcaat gagaattgtt gaagaatggt
    46321 taaattcaag ttataatgga gaaagacatg caagaagatt acaaatgatt gatgatatag
    46381 aaaaagaaaa tttataaata tataatttac aacaaggggg agcgttatgt ctattatttg
    46441 tgcttcaata atgtgtgcag accaaatgaa gttaaaagag gaattagaag ctcttgaaga
    46501 agcaggtgta aaattacttc attgtgatgt tatggatgga atatttgtta aaaacttggc
    46561 tatgggacca gagttattaa aatcaataag tgaaaataca acaatacctt tagacataca
    46621 cttagccaca gaaactccag ataaatatat tgatatgatg tcttatataa agccaaaata
    46681 tatttctttt catgtagaat caagtacaaa tgtaaaagct gatatacaaa agttaagaaa
    46741 ttatggtata gggccagtgt tagcaataag ccctcaaaca agtgtggata aaatagaaga
    46801 atatataagt ttagttgagg gtatcttagt aatgacagta aatccagggt ttgcaggtca
    46861 aaagtttaat ctttctgtat tggataagtt ggataaactt acagagatac taaaagatta
    46921 tgataatcct ccatttatag aggttgatgg aaatataaat aaggatacta ttaaacttat
    46981 gaatggtaaa aaagtagata tatatgttgt tggtacttca gctttattta atgataagcc
    47041 tccaatttca tataaagata aaatagaaga actaaaagaa agtataaagt aaccatgtaa
    47101 taaaattata atatgtttct gtttaatagt aaaaatttag gtggaaacaa gaaattttat
    47161 aatatttaat aaaaattttg gttgagtaag gagaattttt tatgggagac ctatataaac
    47221 tacaaaatgg tactgatatt agaggaatag cgtatgaaaa tgattctaaa gaagttaacc
    47281 taactgtaga agaagtaaaa aaaatagcta aagcgtttca tatttggtta aaagaaaaga
    47341 ctaagaaaga taaagtaact gttgccatag gaactgattc taggataaca ggaagccaat
    47401 ttagaagtac tgttattgaa actttaacta atgatgattg taatgtaatt gattgtgaga
    47461 ttgccacaac tccagctatg tttatgacaa ctattatgga tggatacaat tctgatgggg
    47521 cgattatgat tacagcaagt cacttacctt attactataa tggtttaaag tttttcacag
    47581 aaagtggagg tttagaaaaa actgatataa aagagatgtt ggatatagct gttaaaaatg
    47641 atagtgtaga ctatgaggaa gtaaataaaa aaggtgaagt tatagtaaaa aatttaatag
    47701 aagattattc taatttattg atagataaaa ttagaaaagg tgtaaattca agtaaaaatt
    47761 atgaaaagcc tttttctggg cttaagatat tggttgatgc aggaaatggg gctggtggat
    47821 tctttgctga aaaagtttta cacatactgg gagcagatac gacaggtagt cagtttttga
    47881 acccagatgg tatgttccca aatcatatac ctaatcccga aaacaaagaa gcaatggaaa
    47941 gcatatgtaa agctgtgttg gataataaga gcgacctagg aataatcttt gatacagatg
    48001 tagacagagc tgctattgta ggtaaaaatg gcaaaccaat aaataaaaat gctcttatag
    48061 ctgttatatc atcaatagta ttagaagaac atccaaaaac agctatagta acagattcga
    48121 ttacttctga aggacttgct aaatttataa atgaattaca aggaagacat catagattta
    48181 aaagaggata taaaaatgta ataaatgaag ctataagatt aaatagtgaa ggtgaagagt
    48241 gccatttagc tatagaaacg tctggacatg ctgctttaaa agaaaattat ttcttagatg
    48301 atggggcata tttaatagct aaaattttaa taaaagtagc aaagctatcc ttggaaggaa
    48361 agactataga agaattgata gaaaaccttg aagaagctca agaagagaaa gaagttagaa
    48421 taggcattaa taaagaagat tttagaacat atgcagagga tatattaaaa gatttagatt
    48481 ctcatgttaa gagtattgat ggatggtctg tagcaccaaa taattttgaa ggcataagag
    48541 ttaattgtga tgaatcaaat ggagatggat ggtttttact tagaatatca ctacatgaac
    48601 cattattagc tttaaatatt gaatcaaata aaattggcgg agcagaacaa atttattcta
    48661 agttaaaact tttcttagaa aaatatgatt taaaaggtct ttagggtaaa tagaaagaat
    48721 atatttatat tgaagaataa atgtaaattt gtacctaaga ttacaaatat tacaaaattg
    48781 taatcttagg taacagagat gtaactgatt atggaaacac tatgtgatat tatagataca
    48841 gagagatgaa atatgtgatg aattatagag aaatataatt taaaatgtat taatatcaca
    48901 ccaaaaaggt ataatcaaaa aatatgtaaa tataaacccc aacatttaga aaaagacctc
    48961 taaggaggtt tttttcttgt ttaaataact taaaatatat ttttatatta atatatcaat
    49021 taacttttct aaaagtaagc attaccttaa ataaatcagc atctataatt atttccatgt
    49081 gtgcattttc taattcaatt atattttgag caattgataa accaagacca gagccatctg
    49141 tttttctaga tatatcaccc cttctaaatc tttctattaa ctcttctggt tttagattta
    49201 gtttatcatt tgaaatattt ttaaacgtta atattatatt ttcttccttt ataaacatat
    49261 ctatatagac cctagtgtta ggcatagagt attttagtat attgcaaatt agattctcaa
    49321 aaactctcca agttttttta ccatctatca tggtaaaaac agcatattca ggtgtttcta
    49381 ttataaaatc taaactgcta ttttgaattt tgtcttcaaa ttctacaata ctttgaatta
    49441 aaagttctct tatatcggtt tttgttatat taaattctac atctccacta cttagcttag
    49501 atgcttcaaa tatgtcatct attaataatt ttaatctttg tgatttttta tctaaaatat
    49561 ttatatagtc atttatagtc tctctgtcaa agtcattatt ttttaacata tcaacatagc
    49621 ttaatataga tgtaagtgga gtttttaagt catgagatac gtttgtgata agctctgcct
    49681 tcatcctttc attttttata ttagcatcta cagcagtttc aagaccgcct gttatattat
    49741 ttatactttt ggctaaagta gatatttcat catctcctat aacttctaat ttatacttta
    49801 aattgccatt ttctatattt tttatatttt ttgatatgtt atttatgtaa ttaaacttaa
    49861 cagataaaaa gttgtatata taaaaatgaa aaactgtgtt tacaagtgta aataaaatta
    49921 ctatagctat tgcctcagca taatatatat acataaagaa tatcaaaaca agatattgga
//
LOCUS       DAMKMI010000011        49039 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMKMI010000011 DAMKMI010000000
VERSION     DAMKMI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.011"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(130..324)
                     /locus_tag="PC429_000630"
     CDS             complement(130..324)
                     /locus_tag="PC429_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846148.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(633..2162)
                     /gene="lysS"
                     /locus_tag="PC429_000631"
     CDS             complement(633..2162)
                     /gene="lysS"
                     /locus_tag="PC429_000631"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF3846149.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2177..2656)
                     /gene="greA"
                     /locus_tag="PC429_000632"
     CDS             complement(2177..2656)
                     /gene="greA"
                     /locus_tag="PC429_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF3846150.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="PC429_000633"
     CDS             complement(2765..3733)
                     /gene="dusB"
                     /locus_tag="PC429_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF3846151.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3870..4640)
                     /locus_tag="PC429_000634"
     CDS             complement(3870..4640)
                     /locus_tag="PC429_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF3846152.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4659..5249)
                     /locus_tag="PC429_000635"
     CDS             complement(4659..5249)
                     /locus_tag="PC429_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3846153.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5433..6401)
                     /locus_tag="PC429_000636"
     CDS             complement(5433..6401)
                     /locus_tag="PC429_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF3846154.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            complement(6394..7371)
                     /locus_tag="PC429_000637"
     CDS             complement(6394..7371)
                     /locus_tag="PC429_000637"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF3846155.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            complement(7686..9656)
                     /gene="ftsH"
                     /locus_tag="PC429_000638"
     CDS             complement(7686..9656)
                     /gene="ftsH"
                     /locus_tag="PC429_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF3846156.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9690..11078)
                     /gene="tilS"
                     /locus_tag="PC429_000639"
     CDS             complement(9690..11078)
                     /gene="tilS"
                     /locus_tag="PC429_000639"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF3846157.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11261..11689)
                     /locus_tag="PC429_000640"
     CDS             complement(11261..11689)
                     /locus_tag="PC429_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF3846158.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(11706..12512)
                     /locus_tag="PC429_000641"
     CDS             complement(11706..12512)
                     /locus_tag="PC429_000641"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF3846159.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(12714..13199)
                     /locus_tag="PC429_000642"
     CDS             complement(12714..13199)
                     /locus_tag="PC429_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF3846160.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13250..13909)
                     /gene="spoIIR"
                     /locus_tag="PC429_000643"
     CDS             complement(13250..13909)
                     /gene="spoIIR"
                     /locus_tag="PC429_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF3846161.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(14054..14734)
                     /locus_tag="PC429_000644"
     CDS             complement(14054..14734)
                     /locus_tag="PC429_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3846162.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(14718..15608)
                     /locus_tag="PC429_000645"
     CDS             complement(14718..15608)
                     /locus_tag="PC429_000645"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF3846163.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15726..17276)
                     /locus_tag="PC429_000646"
     CDS             complement(15726..17276)
                     /locus_tag="PC429_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF3846164.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17370..17636)
                     /locus_tag="PC429_000647"
     CDS             complement(17370..17636)
                     /locus_tag="PC429_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF3846165.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17784..18644)
                     /gene="yabG"
                     /locus_tag="PC429_000648"
     CDS             complement(17784..18644)
                     /gene="yabG"
                     /locus_tag="PC429_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF3846166.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18728..19666)
                     /locus_tag="PC429_000649"
     CDS             complement(18728..19666)
                     /locus_tag="PC429_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF3846167.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(19776..19937)
                     /locus_tag="PC429_000650"
     CDS             complement(19776..19937)
                     /locus_tag="PC429_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846168.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19947..20444)
                     /locus_tag="PC429_000651"
     CDS             complement(19947..20444)
                     /locus_tag="PC429_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF3846169.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20559..21269)
                     /locus_tag="PC429_000652"
     CDS             complement(20559..21269)
                     /locus_tag="PC429_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF3846170.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(21445..21975)
                     /locus_tag="PC429_000653"
     CDS             complement(21445..21975)
                     /locus_tag="PC429_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846171.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21992..22222)
                     /locus_tag="PC429_000654"
     CDS             complement(21992..22222)
                     /locus_tag="PC429_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846172.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(22237..23631)
                     /locus_tag="PC429_000655"
     CDS             complement(22237..23631)
                     /locus_tag="PC429_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF3846173.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23757..25010)
                     /locus_tag="PC429_000656"
     CDS             complement(23757..25010)
                     /locus_tag="PC429_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3846174.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(25061..26215)
                     /locus_tag="PC429_000657"
     CDS             complement(25061..26215)
                     /locus_tag="PC429_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3846175.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26775..27344)
                     /locus_tag="PC429_000658"
     CDS             complement(26775..27344)
                     /locus_tag="PC429_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3846176.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            27547..27774
                     /locus_tag="PC429_000659"
     CDS             27547..27774
                     /locus_tag="PC429_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3846177.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27993..28736)
                     /locus_tag="PC429_000660"
     CDS             complement(27993..28736)
                     /locus_tag="PC429_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF3846178.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            28904..29776
                     /locus_tag="PC429_000661"
     CDS             28904..29776
                     /locus_tag="PC429_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3846179.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29776..30441
                     /locus_tag="PC429_000662"
     CDS             29776..30441
                     /locus_tag="PC429_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF3846180.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30462..31319
                     /locus_tag="PC429_000663"
     CDS             30462..31319
                     /locus_tag="PC429_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3846181.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31475..34021)
                     /locus_tag="PC429_000664"
     CDS             complement(31475..34021)
                     /locus_tag="PC429_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3846182.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34021..34707)
                     /locus_tag="PC429_000665"
     CDS             complement(34021..34707)
                     /locus_tag="PC429_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846183.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34790..35779)
                     /locus_tag="PC429_000666"
     CDS             complement(34790..35779)
                     /locus_tag="PC429_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3846184.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(35766..36452)
                     /locus_tag="PC429_000667"
     CDS             complement(35766..36452)
                     /locus_tag="PC429_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846185.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(36952..40158)
                     /gene="carB"
                     /locus_tag="PC429_000668"
     CDS             complement(36952..40158)
                     /gene="carB"
                     /locus_tag="PC429_000668"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF3846186.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(40351..41400)
                     /gene="carA"
                     /locus_tag="PC429_000669"
     CDS             complement(40351..41400)
                     /gene="carA"
                     /locus_tag="PC429_000669"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF3846187.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(41657..44863)
                     /gene="carB"
                     /locus_tag="PC429_000670"
     CDS             complement(41657..44863)
                     /gene="carB"
                     /locus_tag="PC429_000670"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF3846188.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(44916..45959)
                     /gene="carA"
                     /locus_tag="PC429_000671"
     CDS             complement(44916..45959)
                     /gene="carA"
                     /locus_tag="PC429_000671"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF3846189.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(45985..46701)
                     /gene="pyrF"
                     /locus_tag="PC429_000672"
     CDS             complement(45985..46701)
                     /gene="pyrF"
                     /locus_tag="PC429_000672"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF3846190.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(47077..47712)
                     /locus_tag="PC429_000673"
     CDS             complement(47077..47712)
                     /locus_tag="PC429_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF3846191.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(47736..48650)
                     /locus_tag="PC429_000674"
     CDS             complement(47736..48650)
                     /locus_tag="PC429_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846192.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
BASE COUNT        15276 a         9228 c         5384 g        19151 t
ORIGIN      
        1 tttcgtcaat actttttttg ttttttattt taaaaaaggt tataactttt gtttttttta
       61 ataaatataa ttaaaactaa tagatataac ccttgatatt acaaacaata catgcatata
      121 taattttacc tattttgcaa ttttattttc ataagaatat gaattagctc tcactctatc
      181 tgcccaaaca tttatactct taactttatt aacattagtt gtaccctcaa aaatatccat
      241 tgcatcctta caaaaatata tcataacatc aagaaccaaa aatccaacac aaaataaaca
      301 taaaaaatta attacttgaa acatcagaca ttcccccaat aattttttat atatagtatt
      361 ctcataaatt attcttttta ccttctcata aattattcta aatattttca taataaatta
      421 ctttcaatca tattttctaa atataaagtc tcaatcaaca aaaactaata tcaatttcta
      481 acgtttgttg gtaatttata aattaaatat ctaaaagaat ctttgaagca tataaaaagc
      541 attctacact gacattttaa ataaaattta gctatttatt gataatttaa tagctaaact
      601 ttatttaaaa cttagtagaa tgctaatatt tcttagtttt cttcttcttt atttggattg
      661 ttatcaatag gtttcatagt cgggaataat aatacatctc ttatagatgg agagttagtt
      721 aataacataa ttactctatc tatacctatt cctagtcctc ctgtaggagg tagacctacc
      781 tctaatgctt ttaagaaatc ttcatccatt tcaaaagctt catcatcacc taattctctt
      841 tttcttaatt ggtctataaa tcttcctttt tggtcaatag ggtcattaag ttcagaaaat
      901 gcattagcta actcccactt attagcaaat gcctcaaatc tatcagttct tcttgggtct
      961 tcaacatttc tcttagataa tggagatact tcaactggat gatgagttat aaatgttggt
     1021 tggattaatt tatcttctcc aaattcttca aagaaagcat ttattacttc tcctcttctc
     1081 attccaggtt ttatctctat acctttttct ctagcaactt ctagtgcctc ttcatctgta
     1141 tttatagtag aaaaatctac accagaatat tctttaacac aatcttccat actcattctt
     1201 ttccaaggtg gtgtaaagtt tatttctgtt ccttgatagt taactatcat acttcctgta
     1261 gcaacttcag ccatatgtgc aataacattt tctgttattt ccatcatatc agtatagtct
     1321 gcatatgctt gatataattc tattgcagta tattcaggat tatgttttaa atccatacct
     1381 tcatttctaa acattcttcc catttcataa actttatcaa atccaccaac aataagtctt
     1441 tttaaataca attcattcgc tattcttaaa tacattggta tatgtaaagt attatgatta
     1501 gttataaatg gtctagcatt tgccccacct gctatagtat ttaatattgg agtctcaact
     1561 tctaaaaatc ctctatcatc taagtatgct cttaatgctt ttaatgcttt tgttctaatt
     1621 aaaaaagcat tttttacttc tggatttact attaagtcaa catatctttg tctatatctt
     1681 agttcttggt cttttaatcc atgatatttt tctggtaaaa cttgtaaaga tttacatagt
     1741 aatactacag atttagcctt tacagatatt tcaccttttt tagttttaaa gatttctcct
     1801 tcaattccaa ctatatctcc aatatcatat gttgaaaata cactatattc ttcctctcct
     1861 atagcatcta gtcttacata agattggatt cttccctctt ggtcttgaat atctataaag
     1921 ccagctttcc cttgtattct cttgctcatt atacgtcctg ctatttttgt agttttaccc
     1981 tcaagagaat caaagttatc ttttatatcc atagaataat gagttctatc atatctactt
     2041 tctttaaaag gatctctacc tgattcttgt aattttttta gtttatctct tcttacttga
     2101 agtacttcac taaggtcttc ttgaatttgc gcttcttcat tgctttgttg attatttttc
     2161 atttttttcc tccaaattat cttcttattt ctaatatttc aaacttagca actccatcag
     2221 gaacttgcac ttccacaact tctttcgcag ctctacctaa aagtgctttc cctacaggag
     2281 attcgtttga tattttacca tcatatgggt ctgcttctgt cgaaccaact atagtatatt
     2341 caacctcttc atcaaattct aaatccttaa ctttaactat tgatcctatt gtaacaacat
     2401 taaggtctat tttgctttca tctatgataa cagcttttct aaccatattt tctaatttta
     2461 aaattctttc ttcaacttgt gcttgttcct ttttagcttc atcatactca gcattttcag
     2521 ataaatcacc aaaagatata gcaactttta ttctctctgc aacttctttt ctctttacaa
     2581 cctttaagtt ttctaattct tcttctaatt tatcatatcc ctcttgtgtc aataaaaact
     2641 ctttattttc ttccattata aatacaactc cttcacaaat ttcttaggaa ttttcctaat
     2701 tatattttaa aaattttata atcttttttt aatttatgcg ctatattata agtcactatt
     2761 atttttatgt caaggagtct tccatatagt actccaattt cgacacaact tcttgatagt
     2821 cttctatttt gttaatttca tcccttaatt ttgcagagtt tctaagtcct tttaaatacc
     2881 aagcgatgtg ttttctcatt tcccttacag ctacatactc tccatgttct tctactgcaa
     2941 gttttaaatg cttaatggca gtacttattt tttcgttaag agttggctct ggcaaaatct
     3001 cgcctgtatt catataatgg tttattcttt taaatatcca agggttacct tgagcacccc
     3061 tacctatcat tatagcatca caaccagttt tgtctaacat atttattgaa tcttctattg
     3121 taaatacatc accatttcct ataacaggta tactcaaatt tttcttaatc tctgcaatta
     3181 tatcccagtc tgctttgcct gtataaaact gttctctagt tcttccatgt attgctaaag
     3241 cacttattcc acaatcctct gctatctttg ctatttcaac agcatttaca ctattatcat
     3301 cccagccttt tcgaatcttt aaagtcactg gcttttttga gttcttcact actgccctca
     3361 atacctcttc tgcaagctta ggatttttca taagtgctga accatctcca tttttaacta
     3421 cttttggagc tggacacccc atatttatat cgagaatctc atttgaataa tcattcatta
     3481 tctctgctgc tcttcccata aactctggtt ctgaaccgaa tatctgaact gctacaggat
     3541 gttcttcctc ttctatctta agcatttttt tagtattttc atcatcatag caaagtgcct
     3601 taccatttat catttctgta tataaaagcc cacatccttg ttctttacaa attaatctaa
     3661 atggaaggtc tgttacacct gccataggtg atagaaaaac tttatttttc aattctaaat
     3721 ttcctatttt cattgttttt ctcctatttt tgcctttatt ttgtgattta actttaaatt
     3781 cttatttcct cattataata taccacatat agctagaaat ctatcataaa aagggaattg
     3841 gatatataac ccaattccct gtatattaat tattcttggt ttttttcata tattattctc
     3901 aatccttcta gtgttaaaaa accatctaca taatctatac ttttcgtctc tgaagcaatc
     3961 agtttagcta acccacctgt agctataacc ttaacatcat cacaattcaa ttctttcttc
     4021 atcatactta ttattttgtc aactaaacca acatatccat aaattattcc agattgcatg
     4081 gctgatacag tactcttaca aatagtcata cctggcttag ctaattctac tctaggtaat
     4141 ttagacgcac tttgaaataa cgcctcacta gatattttta ttcctggtgc tattgttcca
     4201 cccaaatatt cacctttttc agagatagca caaaatgtag ttgctgttcc aaaatcaaca
     4261 agtatacttg gtgctccata cttttctatc cctgctacag cattaactat tctatctgcc
     4321 ccaacttgtt ttggattatc atattttata tttagacctg tttttatgcc tggacctact
     4381 attaatggct gttttttaca gtactttata caaaagtttt caagagaatg cataacattc
     4441 ggaactacag atgatattat aacatcatca atatcactta tattcacatt atcataatca
     4501 aataggttac ttattaggat tccatattca tcagacgttt tttctctatc tgttttaatt
     4561 ctccagtaat taactaattt gtcaccttta tatataccta aaaccatatt agtatttcca
     4621 acatcaaata ctagaagcat atgtcattaa ccttctttct attttttgct tttcttaagt
     4681 acagtggcaa cagcagctac tactaaagcc cctactattg cttctggcac cccatttgct
     4741 gcggcaagag ccattattac ttttcctgct gaaccacttt tacccattgc atgaagatac
     4801 ttgtcagcat ataagacata tatcattcct aaaactccag ctgtatttaa cagagaacct
     4861 attgcccctg taattaaacc tgatatgtat accttatttg tagccttaat tattaactga
     4921 tatacataat aagctaatac acctatcata actcttggta atattgaaac taatggattt
     4981 ataaaaacaa aagatgttac tgttgggttt gttatagcat ttaagagact tgatatccca
     5041 aatataaatc ctattgctgc cccaactatt ggtccttcca taatagctcc aataataact
     5101 ggtatgtgca ttatagttgc ttttgtaggt ccaactggaa taaatcccaa tggagtcata
     5161 gatagcataa tagaaatagc tgatagtact cctattatag tcatctttct tacatttact
     5221 tttcttactg atgcacttgt gttcatcata aaaaatacac ctccgttcca gctcattaag
     5281 atgccggact acttaatttt ttaatttttt tcgttttttt cttataacta tgagtcaagc
     5341 taccattgta ggatgcccgc tctaacaaga ataattttat caagatgagt tgccattgtc
     5401 aacaatttga caagaaaaat atgctaattt catcatattt ctcgtttttt tactatatct
     5461 ttatatgcca ttatattttc tacactatcc gcttttttta ctgcatttat tatacttttt
     5521 tccacaacct ctacagccaa tgaacctact aatgttatat ctgcttctat ttctcctgtt
     5581 gatattgtga atattgtatc tccatcatga ggtgtatgga taggaaaaat agacttagca
     5641 tatccatcat gtcccatttg agatatcttt ttacatccag ctttggttaa cttagcattt
     5701 gttgcaatta tcccaattgt agtattatct acactaaatc cacctttact ttcaccttgt
     5761 ttcattaatt tatatgtatc aattgtttct gtctttttat catttaaagc accagctata
     5821 accttaccat tttcataaac atctccaaga gcatttacag caattaatgc agatacaatt
     5881 agaccattat ctaattttat agaatagcta cctattccgc ctttcatagc atattcctga
     5941 cctcttatct ttccaacagt ggctccacaa cctgctccat aatttccttg atttaaaata
     6001 tcagtatttg caacttcaca agctctataa cccatcttta aatctggtct acatttaaag
     6061 ctccctactc ctaaatcaaa taaaactgct cctacaacta taggcacttt acctacacct
     6121 acatcaaatc caattccttt ttcttctaag tatctactta caccacaagt ggattcaaga
     6181 ccatatgcag aacctccagc caaaactact gcatgaactt tttgaaccat atttacaggg
     6241 tctaataaat cggtttctct agtacctgga gcagagcccc ttacatcaac accgcaagta
     6301 gctccttctt cacatattac tactgtgcac ccagtaagac catttctatc ctcaacctgc
     6361 ccaaccttta agccttctat atccaaaata ttattataca tatccttttt ctcctcttat
     6421 agaaacttca cctgaaataa tttcttttat acttccatca cttctttgaa ctaataaatt
     6481 tccctcttca tttatgtcta aacatttcac tagttctcta ttatctcctt ttaatacata
     6541 tatattttta tctataattg cagaataatc tctacatatt tttaaggttt tttctttgga
     6601 attatttaca gtataatcta aatataattt ttcaaattca gtcaatacat tcctaacaat
     6661 atctactctg gataacatat acccttcttt atacaatgat gtggctttgt cagctatttc
     6721 ttgttcaaaa tccattattt taacatttat tccaattcca agaacaacat aatttgttct
     6781 ttcaatttca gcagataact ctgttaatat accacatatt ttttttccat ttaaaataac
     6841 atcatttggc cattttatta atgtatttgc tcctagattg tttaaagctt tcgttacact
     6901 tgctcctgct attaaagtta taaatggggc tttgaatggt tccatatctg gttttaatat
     6961 aatactcatc cagattcctt catgaggttt tgaatcccat cttcttccca atcgtccttt
     7021 ccccatatct tgatgttcac ttaaaatcac agtcccatct acactattta tagctatcct
     7081 tttagcatgt tcatttgttg aatctatagt gtcaagatgt tcaatatttt ttccaataaa
     7141 ctctgtatct aatttatagt aaatattctg tgggcttaat aaatcagttg gattttccgc
     7201 taatcgatac ccttttttat ttacagattc tatattatat ccctcttctt ttaaggcttt
     7261 catatgtttc catatagctg cacgtgacac ccctaattgt tttgataatt cttctcctga
     7321 aataaattca gatttattat ttaatataat ctctattatt ttatctctca tatttcacct
     7381 ctaatatgac ataatttcat gacttatttt cttctaataa ataattatac attattaata
     7441 tacttttaat actattaatt ttaaatttcc tttttaatta ttataatggt tgttttatat
     7501 tttatattga tgatttatat aattttacat tttatttaaa taattttaaa ataataaaaa
     7561 aactgaaagt atcaattaaa ttaatacatg caaatataat ttgcttatta atttaatatt
     7621 atactttcag ttttagtatt taatccacat cagataataa tcttaaaatg accaaggtct
     7681 taaaactatt tatctttatc agatttatct tctaaatttt tatttatatc aataacattg
     7741 tttctttctt cttcatcttt ttctaatttt atagttaatt gttcttcaac ttctttagaa
     7801 gaattttcct ctgtaactgc attttcaatt tcattcagag gtaactcttt attaaatgct
     7861 tttacaaatt gttctgcatc taaagtttca tatataagta aagcttgtgc tacatactct
     7921 aatctatcca tattttcttg taatatactt cttgtcttat catatgcacc atcaactata
     7981 cgctttgttt cttggtctat ctcaaaagca acttcttcag aataatttct cttacttgaa
     8041 aaactattac ctaggaatac ttcatcatca ctgtcaaatg acattggacc aagtttagaa
     8101 ctcattccat actttgttac catacttctt gcagtagctg taactctttc taagtcattt
     8161 gaagcacctg tagatacatc cttaagtact agctcttctg ctactctacc accaagtaaa
     8221 actacaatat tttctttcat ttcatttttt gtagcataga acttatcttc aacaggaagt
     8281 tgcattgtaa accctccagc tcttcctctt ggaactattg taacttgatg aacaggactt
     8341 acttcttcaa gtacatgggc acaaactgca tgccctcctt catggtatgc tgtaagtctt
     8401 ctttctttct cacttattac tttcgatttt ttagccacac ctgctataac ctttgttata
     8461 gcttcttcta tagtttccat cttaattttc ttttctcttt ttctagctgt taatattgca
     8521 gcctcattca ttaagttctc tatatcagca ggagtaaatc ctggtgttct tctagccaag
     8581 acatccattt ttacatcatc acttaatggt ttatttcttg agtgaacttt aaatatagcc
     8641 tctctacctt ttacatctgg tgtaccaaca acaacttgtc tgtcaaatct tcctggtcta
     8701 agtaaagctg ggtcaagtat atctggtcta tttgtagctg ccatgattat tataccttga
     8761 tttaccccaa atccatccat ttcaactaga agttgattaa gagtttgctc tctttcatca
     8821 tgacctccac caagacctgc acctcttttt ctaccaactg catcaatttc atctatgaat
     8881 atgatagctg gagcactttt cttagcctgt tcaaataaat ctcttactct tgaagcacca
     8941 accccaacaa acatctcaac gaaatcagaa ccacttatac tgaaaaaggg aactcctgct
     9001 tctcctgcaa ctgctcttga tagatatgtt ttaccagttc caggaggtcc taccataagc
     9061 attccttttg gtattcttgc acccaattca atatatttct taggattctt taagaaatct
     9121 actacctcct gcaaatcttc tttttcctca tctagtccag ctacatcttt aaatgtaact
     9181 ctagtttttt catcatcttt atgaacctta gcttttgatt tgccaaaact cataaccttt
     9241 ccgcctccac cttgagactg attcataaac acgaaccaaa taatcaccat gaagaatatt
     9301 aacagtaatg taggcaacat ttcgacaaac caaggtgtag atggcttagc ttcaccacca
     9361 aaagttagtt tcccagcctt agcttggtct aacacctcat ctgcaagttt atttcccatc
     9421 acttctgttg gaacatatga cttaaacttt gtatttgtat cttttatagt accttctaca
     9481 gaagtttgat ttacaaagta taatcttgat atattctcat ctgttaattc tctatatact
     9541 tcagagaatt ttaaatcttt tattttttct gtaggcttac ctgagaattg aactatacct
     9601 actataatta taaaaacaag tagataaaaa cctgcccctt ttagcaactt attcaatata
     9661 gaagccctcc tttcatacat tgctttgccc tataatttgt taaaattaat ttttaatact
     9721 tcttttgtgt tttcatcaat tttataattt tcactgattt tataatctcc aacacatatt
     9781 attccttcac tatctgtgat tataggaatt ttacatcttt cttctcttgg gatttttaaa
     9841 tctatgaata aatctttcac ttttttactt cccatagcaa gctttatctt atcaccttgc
     9901 cttctgcttc ttattactat accccctttt accttattaa aatcaaaccc tttagatgaa
     9961 ttgtctaatt tcatactctt atacctatct atgctcatta cttgggtctc aataatttgt
    10021 tttaattctt ttattttaat aaacccatta ctaggtacat tgtaataaaa ttcaatttcc
    10081 tcattaacaa tttcctcatt cgttaatatt atactgtctt tttttctata aacaaatata
    10141 tttcttggaa gagttaacat tttatttact tttgaattat cttctaaaga cattatatct
    10201 tctatatgtc tttggtcaac aaaattagta tctcctagta tatgttttat ggaatttcta
    10261 agaactctaa ctttgatagc atcatgtaag caaacaaaat cgtctaaatt tatctctaca
    10321 aagccttttt cttttatatt tgaaacttct ctaaatttag cttctgcctc tttctcaata
    10381 taatcgttat cactttttaa actattgctc atcctcacta tagattctat tacatttgaa
    10441 ttaaaattat ccttcatata tggtaaaagg tctaacctta tcttatttct agtgtatata
    10501 ttttctaaat ttgttttatc tatcctcgga tttaaattat aagcttcaca atactcctct
    10561 atctcatttc tttccacgtc taatatgggt ctaattatgc aattatctct aatataatca
    10621 attcccttta agccttttaa acctgttcct ctcatgatac gcatcataac cgtttcggct
    10681 tggtcattga gattatgacc tattgctatt ttattggctt taatcttatc cttaatttca
    10741 taaaacattt catatcttag cttccttgct ccttcttcta atgataatcc ctcattttca
    10801 caatattttg gtacattaat agactttaca aagaatataa ttcccatatc ctcacaaagt
    10861 ttagatacat ataatgcatc tttttgagct tctatccctc ttatttgatg gtttaaatgt
    10921 gctgcatata tttctatatt aaaatctttt tttaatctat ttagtacatg caataaacaa
    10981 actgaatcag gtcctccaga aagacctaaa actattttat cgcctttttg tattaaatta
    11041 tgtttattta tagtacttag taccttatca aatatcatat ttatccctct tacacttagt
    11101 tttattcact tttaagtata ctttctataa aaaaacgcta aacccttgta tcaattttaa
    11161 gaaattaaag tttaatataa atataataat accaaataaa ttaaataaaa ggtagttttt
    11221 tcactacctt tataaaattt ctggaaaata gccatagtta ttatttagta cctatgtata
    11281 tatctatttt attcctacct aaaaataaat cttgtacctt tatattatag tctattttca
    11341 attttatgtg ttccccttct tgtatattta tatctaattc tcttgtatca atatcaatta
    11401 ataaagtacc ttgtggggtc acatactttg tagtactact ttctccacat ttaaacagca
    11461 ttgttgagtt aatagcacca aactttttga tactaacttg atttttaagt atctttatag
    11521 tattagttat ttctatatct tcttcttttt ccttgtaaat aacatatatt ccatcatttt
    11581 tataaaaaat tttaccaaag gcagtcatct ctatggtgtc catatttcct ttttcatcat
    11641 attgtctagt atttatactt atttttacac tcaacgattt ttcctccata gattcttcat
    11701 ataaactagt atttttgtat ttctactttt tgaatcttat caattttttt ctttaagaac
    11761 tctcttgcta tatctgaaaa tttttcaggt atatctgata cataatattg ataagttcca
    11821 tgaatttctg tattgtttat aatattttgc acctctaaaa tcttttttaa atcctttgca
    11881 gtctctttag ctggattaac aagttttata tgctctccaa cttcttcacc tattgttctc
    11941 tttaaaatag gataatgagt acatcctaac actaaagaat ctatattttt ttctttcaat
    12001 tcttgtaaat atatctttgc tgtcagttta gccacttctg tatttgccca tccttcttca
    12061 acaataggca caaataaagg acatgcttta ttaacaattt ctatactctc atctatttta
    12121 gatatttcca agttgtatgc ttttgaactt atagttcctt cagttcctat tattcctaca
    12181 attttgtttt ttgtagaact tacagctgtt ctagctcctg cttctataac tccaattatc
    12241 ggtatatcat atttttcttg agcttctttt aagcttcttg cagtagcagt gttacatgct
    12301 ataactatag cttttacacc ttttgttttt aaaaagttta tagcttgaaa agtatattta
    12361 attatagttt ctttggatct tgaaccatat ggtattctgg cagtatctcc aaagtatatt
    12421 atatcctcat ttggtaatat cttcattatc tcttttagaa ctgttaatcc acctaatccc
    12481 gaatcaaaaa ctcctattgg tttattgttc atctttatac aacacctact ttacattttt
    12541 gtgtttaaca acaacttatt tatcgctaaa atttatattt atattcataa ttaatatatt
    12601 attacctaaa tgattattat agtttatttt acacaaaaaa taaaggagct atcttaaatt
    12661 agaaaataat ttctacaaaa agacaactcc tttcactact taaaaattta aatttatttt
    12721 gcaaacacat gatttcctat tctcatataa ggattcaatc tatttatcca tttgcaagtg
    12781 gctatttctg gattccaaaa atatactgct ttattagttg ggtcagttcc ataaagagct
    12841 tcctgagctg ctttacgtgc tgagtctgtt ggtgccatct ttattgaacc atcaagcaca
    12901 actgagaaag catttttttg atatatgact tcttctattg tgtctggaaa tctagggtcc
    12961 aatactctat ttattactac agctgctaca gcaacctgtc cctcatagct ttctcctcta
    13021 gcttctcctg caaccaactt agataataac atcaagtcat tatcagataa atttattact
    13081 tctttctttg ctatattttt ttcttgtttg tttactttct taatattttc tgttggttca
    13141 tatgcaaata cctctttaat aggcataaat ataaaagtca aagctaatag tgtagccatc
    13201 agtttactcc acctgtccat gattaaaacc actccttaca ttcttatttt taaaattttt
    13261 tctctaaatc cttgactact tcaagtactt taaatttaat ttttactcta cttgtttttt
    13321 ttggtgtatc ttgttttatt aacttatatt ctttatcagt taaaacttcc ttcaacttat
    13381 catctgtaga tttatctatc actccattag attcatcaac aaaacatagt ggagggaaca
    13441 taacacacca ccagtttttt ccttcacctt tcccaatgat aatctttaat gctttatatt
    13501 caccagctgg aagaactata ttagaatatt gtttagtagg gaaattgcta tattgtatac
    13561 ctacctttac actgtaatta tatccatttt taagaataat atttttactt atttcttcca
    13621 aactactata ttcctttttt ataatagctt cactttcttt tatacttttt gaattttgaa
    13681 gctttggctg taaatatttt attacttcat ctctaacttt tagtttaagt tcttggtctt
    13741 catctgtatt gctgtttgct ataacatgaa atcttattaa tttatcttta taatcttttg
    13801 atatattgtc tacttttttt acctcaccat ttattactat tgtcattatt gatatgactg
    13861 atataagact taatattaat agtcccaatc taatcttaat ttttctcatt gaaaattccc
    13921 cctcaagata aatttatatc atcaattatg tccaattaaa tttaatttat tcataatatt
    13981 ttttaatact aataaaaaaa gctatcttaa aatgattaca atcattttaa aatagctttt
    14041 taaaccttat attttataag tctttactaa tcatagcctt atctatcata aactgctctg
    14101 ctttttctat atgctctgta gcatacttct tggatagctc actatcacct ttaattatag
    14161 catcaagtaa atatttatgc tcttcaacaa tattatgtga tgtatcataa gcagacatat
    14221 aggtaactct aaatctatat acttgttccc acaatgagtt tattaattgg attaattttt
    14281 tattattagt tgctttaaat atacattcat gaaattctac atcaagtttt aataacgcat
    14341 ctacatcgga ttccaaagtg ctttttttaa attcttctga tatatcttta agctttttaa
    14401 tatcttcatc actaattctt tcagcggcaa gatatgctgc taagccttct aggcttgctc
    14461 ttacttctaa tacatccata atatccttta atgacatatt tgccacatac gcaccttttc
    14521 taggcaacat aacgactaaa ccttctaatt caagctttct tattgcctct ctcactggag
    14581 tcctacttac tcctaattgt tctgctagtt gcacttccat tagcctttga ccaggcttta
    14641 attttccttc taagattgct tctcttaaat tctcaaagac tacatctctt aatggcttgt
    14701 aattatccaa gttcagttta gttaaattct ccacaaattt caaccccttt ttgactgcta
    14761 ttaacaacat acacctgttt atattttttt aataattctt tttttccaat tagagcatct
    14821 tctttatttt taaatagtcc aaaaactgta ggaccacttc cactcatcat agaacctaaa
    14881 gcattatttt tcatcattac ttgttttata tcacttattt ctttgtgttt acctatagta
    14941 acattttcaa gaatatttac catgctttct gatacagctt ttatatcttc agattttaaa
    15001 cactctatca aatatttatt atttggtctt tttttaatat tattcaaatc taatccttga
    15061 taaacttctt tagtagaaac gaataagtct ggtttgcata tcaatatatt tgtatcacat
    15121 ggtaaacctt tgatgtttgt tagcttttct ccaatacctt gagctaaggc aggtcttcct
    15181 gatatacaaa aaggaacatc tgcgcccaaa ttaagtccta tttcttttaa ttcatcttca
    15241 gataatctaa gttcccatag gtgatttaaa cctactaata ctgcggctgc attagaactt
    15301 cctccagcca tccctgctgc tacaggaata tttttttcta tgaatatttc tacacctttt
    15361 tttatataaa atttattttt aagtattttg gcagctttat aaacaatatt atcttcatct
    15421 aaaggaatat ctaaactagt gctttttacc tttatttcat cttcgtctaa ttcttttatc
    15481 tttactatat cgtataaatc tatcgtttgc attatcattt caacaaaatg ataaccgtct
    15541 tgcctttttc ccaagacatc aatggataaa tttatttttg ctctactctt tagtcttata
    15601 aaatccattc tatcctcctt gtttaaagaa taatgcatca ctaacaataa tattatacta
    15661 caaaaaataa aataataggg tattataata ccctattata cttaattact ttaaattttt
    15721 taatattaat ctactaatac gacttttttt tctaaaataa gacacttacc aggcttaatt
    15781 ggttcatcaa gttttatatc attaagttct gcaatttcat tttctgttgt attgtatttt
    15841 tttgctatat tccagaaagt atctccttct ttgcatatat atacgattat gctaggagct
    15901 ttagataaat catatacacc ttggtcttct cccttgataa taaaattttc tgctttctta
    15961 tctaatgctt cagtaaatct cttaattttg ataatcaagt ctatctggtc cctattcaaa
    16021 tcaacttcga ctttatcaat acatgctgta ttaaacacag atgctgtatc agttagatta
    16081 tccatttcta tatcatgttc aaatggaatt tcctcactaa ttttataaac caattttaaa
    16141 ccttcaactg ggacaaataa aatttcaact tttatgattc cttgtataat gcttttattt
    16201 ccttcaatat aactattttc tatagacatg gttggacaaa cgcttacaat atcttttatt
    16261 tgtatatcat cattatcatt tcttatgcct tctctaacca taaatgtttc ttcactacgt
    16321 cttaaagtct tatttaattg aataggtttg tggtcaaatt ttattatctt ttgaggcgaa
    16381 tatgcatctt gtaatacttc tcttgtaact tcatctgtaa cttttacctt acaacaagcc
    16441 atacaatcta tttctaaaag acctgtgttg ctttcactat tttgcttgaa aatatgatta
    16501 aaatctgaca tagacaacaa tacttcttca gtcataccat cactaactcc agggacttct
    16561 acaaattgag taaactcaat acctactcta tccaactcta caagttcacc ctcatatgta
    16621 caagctaacg gatttatttc taatactcca ccaataataa ctttattgtc agtcactcta
    16681 ctttctttta ctttaacaca tggatttaaa cttattatag attgaatttc ctctgtgttt
    16741 attgttatag tatctcttat agaactttct gctttttcta ttccaactat atcttgaaaa
    16801 caaatttcct ttctgtgctt ttgtatgcct tccacttgcg ccacatcttt tacaatgtct
    16861 aacctttgct tttcaaacaa acttcccctt atattcataa gagctcctac tttaatcttt
    16921 ctctcattca ttatagtaca atccatatgc tctacttctg aaaataacat gtattccata
    16981 tcttgaacaa cattgtcttt ttctattact tcgtttatat ctacttttcc gtctacatta
    17041 gaaatagtat ttttatcatc tgcaatatag attacattat aattaaaact acctctgcaa
    17101 agtatcttcc catctgccat ttcaattttt ttaagtgata tgtatccttc tgtttttaca
    17161 atttcatata catctaattt tttgtctggt acaactgcct ccgcttcaat gaatgtttga
    17221 aattttccaa agtctattct attgtcaact ttaattacat ctttaattaa ttccatagtc
    17281 tacctcctta agtattataa aaatatcaca ctatatttat atttaaggag caaaaaaaaa
    17341 attacaccta tttttaaata ggtataattt taacttattt gtaatttatc ttgatctcta
    17401 aaaacttgca actttacatt agatgttaat aaatctgagt aactataaga tactctagga
    17461 tatccattac tttcatcatc taactttata acaaacacgc ttggataaac tttttctagt
    17521 ataccctctt ttgtaataat ctgctttctt cctttattag ctttcaacaa tattttcttt
    17581 cctatatgtc tctctaagct tactcttatc ttatctagag tttgaacagt agccacaata
    17641 tcaccttctt taacgtttat atgtttacat aatatcacat ataaacattt ttgtcaaatg
    17701 tttaaactaa ttattttata tattttttgc aaaaatgtca atactatttt tgaagatata
    17761 cagtataatt atgttaattt ttgctaatgt aatattgttt ttggcattgc ccatctaaat
    17821 tttcctctgg tttcagaacc accaattccc ttaacacctg taatagtctg ctctataacc
    17881 tcatcaatag gaactactac atcaattctt gaacaagcaa ttttctccac tataaaaaca
    17941 gggtctaatg catgtatcat tatccttcca ggcgaacttg catagtttgc tcctgcactt
    18001 attatttttt cataatttga ttggcatgct cctgcgaata tgacaagatt atccaaattt
    18061 ggttgccatt tacgtgcttc ttttactgtt ttaacaaaat ttgaagagtt tctatagtta
    18121 ctcatatctt ctatatctcc tctttttatt gtcattgcat catgacctgt tattactagt
    18181 atatccggat tatatttttc tagtagtgct cttacttctt tatactgatt ttgctctgaa
    18241 acagctacac ctactgcagg tattccaagt tgagcatata catccaagca aatttttaaa
    18301 tattctttat ctccatctat ctgtagtact tttccaggca ttccatatgt gttaggattt
    18361 aattgaagtt ttggaactgc tctagtcatt cttttttgtc tttctttagc ttttcttact
    18421 gacttatata ataaattttc tacattttta tctattaata tatcttttat atcaggtgct
    18481 tttactaatt ctaggtcatc aatataagca tctgctatta ctctaaatgc aattcccttt
    18541 agtatagcta ttttctcatt attttcatct acaccaaaag agactatttt aaatactata
    18601 tctttgttat gtgactttct agctacaatg tctcctacct tcatagttaa acctccaata
    18661 aatttttatc ttagtttata ctatgttcga tataaaactt tgtttcctaa tgtcaaaaaa
    18721 acatttctta taatacttct tttatcactc ttaaagcatt tttatagtat atcttttcta
    18781 tatcatcttc actaaaccct tcttttttta gaggttcata cagtttaccc atctgagata
    18841 tatcttcaat ctcaactttt gaatctattc catcaaaatc tgaccctaga gatacaacat
    18901 ctattcctcc aacatttaca atatgtttta tgtgtctaac catatcttca agtttacttt
    18961 cacttttatc acttaaaaat aagtggaaga agtttatacc tgtaactcca cctttatttg
    19021 ctaatacttt aatcatatca tcagttaaat ttctagaatg attcatcatt gctcttgaat
    19081 tagaatgtgt agcaattatt ggtttagaag aaatctttgc aatttcataa aatccaccat
    19141 ctgaaatatg agaaacatca accaacattc ccaattcatt catcttatgt accacttctc
    19201 ttccaaaatc agtaagccct ttttctctat attctacttt attatgagga aagctcaatt
    19261 cattcacatg attccaactt attgtcatca ttctaacccc taagtcgtaa aatttcttta
    19321 aattttctaa tttaccttca agtactgcac cttcctcaat agataaaaga gctgtaagtt
    19381 taccttctga ttggtttttc ataatttctt catagttagt agctaaagct atttcatcac
    19441 tatttttttt catttcctca tgaaatttat ttgccatact catgcaataa tcaaaaggat
    19501 gttttacttc ttcagtatca acaaaaagag caaatgtttg agctaatgaa tccccctttt
    19561 tcaatctgtc tatatcaaca gaatatttat tagacttaag ttcacttgtt tctacatttt
    19621 ccattaattt tgctatagta tcacaatgta aatcaataaa tttcatattt ataccccctg
    19681 tatattttat atttatatct aaaaatagaa atagttgtcc ttatagacaa ctattgacaa
    19741 ctatttttta atattctttt taatattact taatcttatt cttcttttaa gttaggtatt
    19801 acttttttaa cttcttcaat ttgatgccat gttttatcta tattattttc actaatatat
    19861 ttttttaaat cagttaaaga tgtaaaatct ttaactgata aattgtcatc atcatctgca
    19921 aatcttaaaa taaacatgag actcccttac tttccacaac atttcttata tttttttccg
    19981 cttccacatg ggcatgaatc atttcttcct atttttgctt ctttaactat tgttttagac
    20041 ttcttataag cctttattat ttcttttctt tggtctgctg gtagtatatc ttcccaacct
    20101 tgtaaattat atagccaatg agcctcaaca gttaccatat tgttatataa tttttcccaa
    20161 tctattttta tagaaacttc tttatcagct tctacttctt ctaaaactat atcttctttt
    20221 aagctttcac ttattccatc tatgaatccc atgaaaaatt cattagtagt tccaaatttt
    20281 tcagccaatt cacttacttt accttcaaca acatccattt tattatttaa tatttcattg
    20341 tatattccag cttcaacttt caaatattct tcccagaatt ttacttctgc ttcttgactt
    20401 tcatgattat cacttaaatt tctccattca gtatataaac tcatactttt tcctcctaag
    20461 tatacctata tttttatagt ttttatagta taaaatcttt tagttagatt ggaagtttct
    20521 tctttttgat tataccatat tctatttaaa gttaactctt atattatatc ataattaaac
    20581 aattctttta atacttttat agcatactct tggtttataa aataagggct tgtcgcaatt
    20641 gttattactt tggctttttt aattaagttt tttatcttgt aaaatttgag ttcaaatggt
    20701 atatagtcca tatcacttga aaatatatcc aaatctatgt caagaacaaa ctctgattct
    20761 acttctaaat caaatccata tagactatct attattatta attctgaaaa tatgttatat
    20821 tttaaagcag gttttataaa acttcccaca tttaaaactt catttgtata tctaaataca
    20881 tcttttagat tatttacatc tacatcatag ttctcaggtt ctctagtatc cttatgttgg
    20941 tctacatgca ctaacttgca tccttttgta aactcaccct ttaacaaaga ctttgcccaa
    21001 aaataaaatg catggttatg attgtcaaac acatatatct ttttatttcc caggacatat
    21061 tcgaccatat tctccaatcc ttttgctttt atttccttat tttcatctac ttcattaaat
    21121 actacctcat tgcctaacct tacatcattt aatgtaccat ttataagttt tggaacgtat
    21181 atgctcttat tttctctttt atcatatgaa aatacattat ttcctactgg tttttctata
    21241 taaaatccac tatattcata attttccata aaactttatt tcctccattg ttatataaat
    21301 atataagtta taattttaaa aattaaaatg cacttattgt aaatcatatc atctattata
    21361 gaaaacatct tatataacta aatataatat cttctctcaa tatgtattat acaataagtg
    21421 taaaatatat ttatataaaa tatactacat tcctataaca aaaccatcag cttcaattgg
    21481 aagataacta actcttctat tcaactccac ctttccaaaa aatactaccc aatatggtct
    21541 aaaaatactt tcaattttaa ctatttcaat ctctgggatt cctcccataa acctatgagt
    21601 taatctcata gccattttat ttgcctttgc ttttactctt cgttcgtcct tatcactata
    21661 ctgcacaata ttacaatcca cattttttac aacttcatat gtaggaaaat cttgtactat
    21721 actaccagaa cctgtagaac cattcactaa aatttttaat tcatttatat cattagaata
    21781 tctcctattc ttgaaaatgg atttcgtttt gtgaatttta agtgtgataa ttttaaactc
    21841 tatataatga agtctaactt catgagcata tttgttcttt aatattagct tactaaataa
    21901 agaatttttt ccacttttag ctatctctat agcttcttct tttgtaattc ttataggtaa
    21961 cgctcttata tccataaaat cattctcttt cttatttttt tcttgtcatt tttaaaactc
    22021 tatatatata atcaaaaaat attacaatta caactgacat aactccatat ggaattattt
    22081 ttccaactat aatatcattt ctagtcaata taccataaaa tataaatcct gttattaaaa
    22141 agtacaataa tactagaaaa ccgtctattc caagccattt tatttctaaa tcttttttta
    22201 ccttagacac taatttagac atagcagttt ctccttttat agattatttt gtgtatttaa
    22261 attttcacct attctattac caatcatcca tgcaataaat gaaaaaggta tagctatcac
    22321 aagaggctct aactcaaagc tctgaactgg aaatatttta aaataaccaa aagtcactcc
    22381 accaacaatc attcctaaaa taccacccat agatgttctt cttccatttc caagaagccc
    22441 acctactata ggtacaaatg ccgaacccat ccaaatactt cctacaaata tacatgcatc
    22501 atatacattt tcaaatctaa atgctacgct aagccccata actgctgcta aacatacacc
    22561 tattctagta aactttacta attgtttctg agataactct ttttttgtaa ttcttccata
    22621 tatatcattt gctaatgtag ccccagctgc tagataatag ctatccaatg ttgatattat
    22681 agaacctaaa attcctatta taaatattac tcttattcct tgtggtaatg tatctaaaac
    22741 taaagttata tatccagcag ctggagccat ttctggataa gtagctctta ctataagtcc
    22801 tgtcatagtt aacaccatgt caaaacatat ccatagtgaa aaacaggtta ataaggctct
    22861 tcttccaacc ttaggagaat ctgctgctga aaatctttga taaaaagttg ggtctgaata
    22921 agctccaaaa gaatatacaa ttaacatcaa tgctttcata gctgacatac cctttgtagg
    22981 gtgcataagc tgtggtgcat ctttgagtgc ttctgtaaga ccttgaaacc ctcctatttg
    23041 ttcaaattga cctggcataa ccatagttaa accaaatacc atacaaaaaa acaataccat
    23101 atctgtaatg gcaactccca taagcccacc aagtactgta agtacaacga ctactcctac
    23161 cattattaat cctattaatt ctttatctat tttccatgct acgttaccta tagttcccat
    23221 agcagttatt tctccaagtt gtgcacaata tataaacatc aaaattgccc caattatagc
    23281 aacatgcttg ccataaaaac tttccagtaa atcaggaact gttatattgg ttcttatatg
    23341 aatccttgga ccaacccaca atgctaaagg cattctccca atatgtgcag ctattgacca
    23401 aactgcccat gcagatattc catatatagc cgcatactct acactaccta ctgtaccaac
    23461 tcctccatac catgatgcaa ccaaagttcc tactattaag gaccatggta aacttcgatt
    23521 tcctaggtaa taactttcca tatcttttac atttctagaa aaataataac ctattcctaa
    23581 taatataaat gcaaatataa ctataatccc attttcccaa aatgttaaca tatctaatct
    23641 ccacattctt atattttaat ttttaacttt aatatctaaa tatgaacaac tctacccatt
    23701 tccataaaaa taggtagagt tatataataa ttataagtat ttaagtaatc ttgttattaa
    23761 gcaattttat tgtttaaacc tttattttct cttttattta ttttatttaa agcataagta
    23821 gcaaatatcc ctataactgc acatactaca gataaaataa atatatatgt atatcctgtt
    23881 tgtccatatt tatctaacca attacctgca atagtgtaat aaaacgcatc tggagaaaat
    23941 cctataaatg aagcaaatcc aactactgct cctgtagttt ctaaagatat attagactct
    24001 gaaacagatg caaagtatat acctctaaat ccaaataaaa ttacacttag tattatcatg
    24061 tttattacag ctatccatat aaatcctgta ctcttaggaa taaccaagta agcagctgta
    24121 cttactgtca ttaatataaa tcctacaaac attactctta tagatgaacc aactttatct
    24181 gttataacac cagcaactgg agatgctccc atttttataa cataagttct tattattgat
    24241 aaagcagaaa ctagagtcat tggcattaca taaacttctg ataagtatgg acttaaataa
    24301 gttaaacttg agaatactaa ataagttgaa aatattataa gaccaattag ccaagccttt
    24361 ggcattgtaa ctacttttaa caatgactta atacttaatc cttcatctga tgctccacta
    24421 tcttttttct cttccactaa gaaaaataga agtataccac atattataga tgctgcagaa
    24481 tataaccata ctacatattt gaatcctact gttatttcag caaatttacc aaagaagtaa
    24541 agacctataa aagaaattaa tgtacctgat acacctgata acccttcata aaaaccaaat
    24601 attcttcctt gctctgactc acttccttgc attctaacaa ccttaacaga ggctgagaag
    24661 aaagttaata ttgttgtaaa tccccataat acaaatatta aaagtatcat actatatgat
    24721 gggaatgtag agaaataaaa tccaagtatt cctgaagcaa ttaaagaaaa agttattaat
    24781 tttctaactg agaatctatc tgcaaaccat cctcctataa aataagatac cattcctact
    24841 aatccatata aactcaataa gttccctatt tgtgtatgac ttaatgctaa agcttgttgc
    24901 attgggtcat aaaatgatga tttcatatat ggtaaactat acatagctgt tgttccaaat
    24961 gccaatatga atattatcaa aaatctttta acattatttt tgctttccat tgattctctc
    25021 tccttaaaat tttattgttt ataatctcat tcatataatt ttattttaat ttttctattg
    25081 cttgtttaaa atcatttatt atatcatttg aagattctaa tcctacagct attcttatta
    25141 gattttcccc attcatttga actaatgtat gctcttcccc taagcttgtt gctattgttg
    25201 caagctctaa agaattcatt aatcttctac tagcttggtc tcttgtcaat ccattgatac
    25261 catccttaag tttaaatgaa actacacctc cacctaaacc attcatttga gttttacaaa
    25321 gctcatattg aggatgagat tttaatgctg gatactttac caattccaca ttctcttctt
    25381 cctctaaaaa ttctgctact gctaatccat tttcacaatg ctttttcatc ctcatatcta
    25441 acgttttcat accccttaaa atcatccatg cactcattgg tggtaaagat gctcctgtaa
    25501 aacaacataa acttggccat ctaactaaat ctattaattc ttttgaacct gctactgctc
    25561 cccctaaagt atccccatgt ccatttataa acttagtaag actatgtatt actaaatctg
    25621 cacctaaatc tattggtctt tgtacaattg gtgtcccaaa agtactatca actatagcta
    25681 agcttttatt ttcatgagca atttctgcta ttgtttttat atctactaag tctaatgatg
    25741 gatttaaagg actttctata taaactattt tagtatttgg tctcattgct tcttttacat
    25801 tttgtggatt tactgcatct acaaatgtaa cttcaactcc aaatttagat aacaactcag
    25861 acatgaataa tttagtgtgt gaaaatattc catctgaact aactatatgg tcaccacttt
    25921 ttaccaatgc taatatagta gatgttattg cccccatccc tgatgtacta gatatggcac
    25981 tttctgcatt ttcaagcata gccaacttag cttctaactc tgtaagagta ggattacact
    26041 ctcttgtata cacatatccc catgtcatac acaactcttc aaaatgctct actgtctttg
    26101 cagcaaatgt tgctgtttga catattggtg tagccaatgc acctgttgtt gggtctgcac
    26161 tatgtcccca atgtattatt tttgtctcta aatcttgatt aatattgtta ctcattttga
    26221 tttcctccca ttttgactta tttatacaac gttttcccta ccaaatttca tcaatttagc
    26281 ttagtcttaa tacatttttt caagtatact tttttatttg ttataatttc taaaaatatg
    26341 acacttattt tgttttttta cagttctaat tataaactct atagtaacta tagagtcaat
    26401 atgtttttga tatttttttt acaatgttta aaatagtcag tacaaaaata ggtatatctt
    26461 agaataacta aagtcatcct aagatatacc tatttttata tcacaatata aataattaca
    26521 tataagcatt agttttttta tcataaatag atttttcaag atttatcatc ttttcataaa
    26581 tatattatag ttaaatattt taatattacc taacatctac aataaaatat actcttaaaa
    26641 taaaattata taattaaaaa tactctataa aagctaaaaa gtttttctat tcgctacatc
    26701 taaaaataat tagatagagg tataaatgta tttagtttat ttatacctct agtcttttat
    26761 aaattatatc tctactaaat tgatttttta gcacgaataa gtgtgctctt ggagatacca
    26821 gtcatcttca caacctctct atatggctta gaatttaata accccaatgc atattgtatc
    26881 tgttgattgc tgtatttctt tggcctacca tctttaaagt cctttttctt tttagcaaat
    26941 tcttttccta attgagtacg ttctattatc atatttctct caagttctgc aactgcaagt
    27001 aatgtagtca aaaagaattt acccattgaa gtattttcta aaaggcctat atttaaaata
    27061 tgaacactta cttctttagc aaataaacct tctattagtt gtattccttc tgatgtactt
    27121 ctagctagtc tatccagctt agttgcaaca agtaagtctc ccttttttag agatgatata
    27181 attttttcaa atttaggcct gtttgtagta gtgcctgtat aagtttcctt tattatttta
    27241 gcatcattat atttattcaa aatttcttgc tgttgctgat acaaactgtt gttgtctgtt
    27301 tgattttttg tacttacacg gcaatatcca tatataataa tcatcacttc ctaacataaa
    27361 gattttgtac agaagttacc cagaagcttg tttttactta tctttatcca ttttactaaa
    27421 acgatttttg ggtgtcaata ctgttaactt ttgacaccat aataattcct ttataatttg
    27481 tttgactatg tcaaaaatta ccaatataat gaaattaatg agtttataca aattggagga
    27541 aataatatga atagtctgtt aaaagaagca atagatgaat taaaaaacat ccattgcggg
    27601 gaagtttttt tattaaaaga attgttcaaa ggctatgaat ggaacaggtt ggagatgaat
    27661 atcagattga aattaggaaa actttttttg tatgaagtgg taactaatcc caatttaaat
    27721 gtagaggtca tcaataaaaa ttctgcttat cagcaaatgt ataaaaaaaa atagtgactt
    27781 aaatgtatat aatagtataa tcttcttatt gattcaaaag ttaaaacttt taatacaaaa
    27841 caatcattct attttaacat ttatcaatat gtctaaattt atataattaa cacatacgaa
    27901 tagtattgtt taaatcacat tatttaagat agataaatct taaccctact ccagcataaa
    27961 tactaaaata gggcttagaa ttatctatat ttttatccca taaaatgctt agctcttcct
    28021 cttgattgaa catatatatc tggggaaata caatagaagc gtaaatttat aaaatttcca
    28081 aagtcagaaa acttcagttc aaacttaggt ataccaaaac tttcaggtgc tatttcgtca
    28141 acgccaaaat agatgataat tccattatct gtaagataaa atgcttggtc ttcaggtata
    28201 actaactcaa cattagaata atacatttct ctattttcat ttatttgaga tttaatgtaa
    28261 tcagtaatta tattaatata atcaactcca tctctaaaaa tgtcattaag taaaagttct
    28321 tttccagttg tcaaatctat attgtaatta ttcaatgtat tgtatttagg cccatagtta
    28381 ttagcaaacc ccataaggtt taatatcata ctcagtacat aatttttatt aaaagtaact
    28441 gcgtaactgg tagataccca atagctttgt tttggaagtg aattttgaga agttgcgtta
    28501 ttatattctc gttcttcttc ctgtaagcca tttttaaaat tatttacagt cattcttaca
    28561 gcattgttta tgttatttaa aataactggg tttacatacg tggattgatt tctaatattg
    28621 tatataacag gatactttac atcatatgaa aaatacttac ttctttcaaa atcttcatta
    28681 aattgtacat ataatatatt atatgtacag gtatttgtag attgataatt ttccattaaa
    28741 atcctccccc ttttataaaa aaatcagata caatataata tgcttaaaac agtaataatg
    28801 ttaatggtaa atgtaaagaa tagaaagtat ctaaaacagc aaaaaatgac taattttgtg
    28861 gtataataca taggattgag agttatttta taggaggaaa aatatgttat tattagcaga
    28921 taaatggaaa gattatgaac ttatagatat gggaaatgga gaaaaattag aacgttgggg
    28981 aagttatgta cttagaagac cagacccaca ggttgtatgg ccaatggaat ctgaatgggc
    29041 tttatggaaa aaccctcatg gacattatca cagaagcaat aaaggtggag gtcaatggga
    29101 acacaaaaaa agatatccag aacaatggac tataaattat aaaaacttaa agtttcacat
    29161 taaaccaact ggctttaaac atactggatt attcccagag caagcagcta actgggattg
    29221 gatgatagag aaaataaaga aagctaacag acctataaaa gtgttgaatc tttttgcata
    29281 tacaggtgga gctactgtag catgtgcatc tgctggtgca gaagtatgtc atgttgatgc
    29341 tgcaaaaggt atggttaatt gggcaaaaga aaatatagaa ttatcaggtc ttaaaaatca
    29401 aacagtaaga tttatagtgg atgatgttgt aaagtttgtt gaaagagaaa taagaagagg
    29461 taaaaaatat gatgcaataa taatggaccc tccttcttat ggaagaggtc ctaaaggaga
    29521 agtatggcaa atagaagaaa agttatacag ttttgtagat ttatgtatgg gagttttatc
    29581 agataaccct ttatttttct taattaattc ttatacaaca ggattttctc caatagttct
    29641 tgaaaatgta ttgtctacaa caataggtaa aaagacaaat acaggagata tatacggtgg
    29701 ggaattagga ataccagctt ccagagatgg taagatactt ccttgtggta tatttggaag
    29761 atgggagaaa aaataatgat aaaagtcata tatgaagata accatttatt agttgtagaa
    29821 aaaccagtta acatattgtc tcaaggagat aatactaatg ataaagatat ggtaaatctt
    29881 ttaaaagact atattaaaaa aaagtataac aaacctggaa atgtatatat tggtttaata
    29941 catagattgg atagaccagt aggtggagtt atggtgtttg caaaaacatc aaaagcagca
    30001 tctagattgt ctgagcaggt aagaagtaag actttcaaaa aaacttatct agctgttgtt
    30061 catggcaaga taaaatctaa ctcagatata ctaaaagatt ttttatataa aaataaaaaa
    30121 actaatatgg taactgtagt gaaaaaaagc cataaagatg ccaaagaagc tgaacttaac
    30181 tataatgtat tagattttaa agaaaatcta agtttagttc aagttaattt aaagactggt
    30241 cgttctcatc aaataagagt tcaatttgca agcagaaaac atccattata cggagaccaa
    30301 agatatggca aagaaataaa taagattgga caacaaatat ctctttggtc caataagata
    30361 gaaatttgcc atcctactac aaaagaaaaa atggaattta catgtcatcc acctgacaaa
    30421 tatccatgga atatatttta aatctcaaat gaggtgatta tatgagtaaa aaattttcaa
    30481 taggagaaat gtctaaactt cataaaataa gcatacagac actaaggtac tatgaccaaa
    30541 tagatttatt taaacctgtt catgttgacc aagaaagcaa ttacagatat tactcaattg
    30601 accaattttc acaattagat attataaaat atttaaaata tttaggtatg cctttaaaag
    30661 aaattaagtc aaagcttgat gaaaataatg aagaaattct aaaattacta acaaataaaa
    30721 ttaatatagt ggatgaaaaa attaaagaat tagagcttat aaagaaggta ctaagcacaa
    30781 agaaagaaac tatggaaaaa ggtatgaaag tagatgaagt tggtaaaata gtgagaaaac
    30841 atattccacg aagaagtatc ttatctatag agtatgaaga aggattaagt agtgaagaaa
    30901 ctatggaact ttccagaaga caaatagcaa atatattaga agataatata tctgtgttct
    30961 atggtggagt aagtggactt gtatctatag ataaattttt aaaagaagat aaagttattt
    31021 atgacaatag ttttgttact gttgaaagag acttatttga tataaaatca caaaaacata
    31081 taacagaaat ttcatctggt gattttattt gtatgacata taaagggcct tacaaagaaa
    31141 attataccgc ttttaagaaa ctggttgatt atgttgaata taaaagaata cctattgaaa
    31201 attatatata tgaaatacct ataatagacc ctctgtctac aaacaacgaa gaggaacttc
    31261 taacagaaat tcaaatacaa gttaaaaaga gagaaattga cttaagatgt ctagtgtagt
    31321 aatctagttt acttacacat cttaatatag ttgtctatta tcaacatatg atttttctag
    31381 atagtgattc ctaacagtga gattttcaaa aaataggagg aaatatggct aatgctaatt
    31441 ccctcctatt tttaattaag ccatatcatt ttaattaagc catgtcatca cgaagtgcat
    31501 caataatatt ttcttttttt atcttattaa tagtatacaa cattgtaata aatactataa
    31561 ataaaacact aaatatacta attccaatac taaaccatgg aatcacaaaa tcaatatcat
    31621 ctgtaaccat tactttatga attagccatg aagaaattac tgctatagga agtccaaaga
    31681 acaatgctct catgccataa aaggcacact caaaattcat catcttttgg aaatcatgct
    31741 cagacattcc tacagagcga agcatggcaa gttctctacg acgtaactta atattagttg
    31801 aaattgtatt aaacacattg gcaatcgaaa tcagagaaat cataattatg aaagtataag
    31861 caaaaacatt ggcaataaaa atgtaattac ggttctcatc catcattcca tacatattgt
    31921 acagattgta ctcattcgta actccctcac tctcaataat ttgctccatt tctgcaactg
    31981 actgagtagg atttttcgat tgaaagctta aactttcaat tcctgtagaa acaccaggaa
    32041 tctcaaactt atctttggct gaataaggag ccaatactct gaagcttgct ttcttcacat
    32101 ctgaacttct gattcctgga ggagaatcag gtggcactac tttgacaaat gaaatattca
    32161 cattttgtct ctcaggtatc tccaacttgc cattagtttc aggagtgagg ctaacattca
    32221 tagaagaact gttgaaaata tcaataatat tattatcctt tgttaccata gaaacagcaa
    32281 tcagttttgc attttgtcca gtatattctt ctgctggtaa tccaagactt ttaataatat
    32341 tcagataagt tctatcatca agaaattgca tatctactgg taattcaagc atttcactca
    32401 tagaatgtga accctcatag ttcaaataat cgtctgtaag ctcacttact ttggcaacac
    32461 atgaataggt cataagtttc tgatacaaac cttcgtaaac accatttgca gtttttagct
    32521 tatcgtaaag cttcaacatt tcgttgtctt tcataccttg tgtagaaaaa ataatatcct
    32581 ggtcagtaac ctctgacctc tgatttgaca tctgttttag gtctgctact aaagcacttg
    32641 tagatataaa tagaactatg cttaaaacaa gcgacaatac aatacttcga taacgtttct
    32701 tgtttctttt aaagtttttc atagcaagaa ctccctcaag accataaact cgctgtgtta
    32761 gttttgatgt cttcacagat ttagattcaa tttttacctc atttgtctga cgaatacact
    32821 ccatcactgg tgtgttggca gcctttcttg ctggtatgta agcagaaatt aagattgtaa
    32881 ccatacttac cactgctgca acgatgattg cagggataga caatgttaat gtcaacggaa
    32941 cgtttacata gagaatattt gcaaagtttt tagctatgac agaaatcaca agtccaatac
    33001 tgactatacc gattgcaaca ccaattggta taccaatcgc accaatgcaa attccttcaa
    33061 atagcaccga atttcgtaac tgttttgctg tagctccaac agatgaaagt attccaaatt
    33121 gatgtgttcg ctcgttcaat gacatgttaa atgaattata tattagaaaa attgagccta
    33181 gcatgattat ggcaagcaca atgccaccaa ccgagtacaa aaacatattg aacaaattgt
    33241 tatcagaaac acctatgaag cgcaacacat tgtcattgaa aatggaagca tggtctttag
    33301 tgctacttat gtaagagcga atctgatgtg gatttttcaa tgtgacaaaa aggctaagac
    33361 tgtcagtctt gacttgttta tcttctttgg ttatcaaggt atatcctggt gcagaagatt
    33421 cttcaaatac gggctttcta cagataccca caactgtata agttttctta tcctgtggca
    33481 caaaaacttc actcccagca gtgtatgggt cactctgacc aagcttttca ttttcactca
    33541 ttcgtttccc tacatccaaa gaaatagtat cacctactgc aaatttgaca ccaccatctg
    33601 tcgcaacttt acttgaaaca agaatctctc tactattttc aggtaatctt ccagaaatta
    33661 aagttatagg caaagtatca aaagcttctt tgctaaatcc agcaataaaa agatatggtt
    33721 tatctggatt tctgttacct tcaagtgttg catagccaat attttcaaat gttgcagtat
    33781 ttgcaacttc atcattgact ctttgttcct gtacaaaaga agaatccaca tccaaaaact
    33841 ggacatgcca atcaccatat tttataatag ctccatttgt catatagttc atcaaagaaa
    33901 ctccaaaggt agtaactgct gtaatcatag cagctgacag aacaactcct ataattgtta
    33961 caagagttcg agtgcgattc tttttcaatc cttgcagagc aactttgtta aaaatgttca
    34021 ttaacgattc accttcctct catcacgcac tacttttcca tctgaaatac agataattct
    34081 atctgcctgt aaagcaatgt tttcatcatg tgtgacaata atgagagttt ggtgatattt
    34141 actatggctc tctttcagta actggataat ctcttgtcca tttttactgt ctaagctacc
    34201 tgtaggctca tcagcaagca taactgctgg tgcattcatc aatgcacgac caatggcaac
    34261 acgttgctgt tggccaccag aaagttgatt tggtaaatgt gtttttcgtt cttttaaacc
    34321 aagcaattct aacaagtcat ttaaccgttc ttgattgacc ttacgcttat ccataagtat
    34381 aggcaatgtg atattttcta ctacatttaa tgtgggtatc agattgtgaa actgataaat
    34441 caatccaact tgtcgcctac gaaaaatagc aagtttttca ttgctttggc catatacatc
    34501 ttgcccctcc agatatatct ttccacttgt tggtacgtct actcctgcaa tggaatgtag
    34561 tagtgtagat ttaccagaac cagaagaacc aatgattgca gtaaactctc ctttttcaat
    34621 agtaagtgag acatggtcaa gtgcagtaac cttattttca ttcttaccat aaaccttaca
    34681 taaatcttca atctttaaaa acgccattat ataagtctcc tttcatattt tataaatata
    34741 tcataggact ttcaacttac atttaagtga catttaaatg agagattcgt cactttggaa
    34801 aacgaataac aaatattgca ccaccttgtg ggtgattttg tgcagtaatt gttcctcctt
    34861 gtctcataat aatcgtctta caaagtgcca atcctattcc ataaccggct gtgcttgaat
    34921 ttttcccacg ataaaatctg ttaaatagac atggtaaatc ttctttttta aagcctgctc
    34981 cgttgtcatg aatagtaagt tgagtaaaca aaaagttgtc ctcacaaatg atatcaattt
    35041 ttccattatc acccacactc tccatgcagt ttttaaatat attttgaatt gcttctgaaa
    35101 gccaatttaa atcacccaaa atccttactc cttttggtat atcaatatgt aaagtgatat
    35161 tgtgcagttc catgggaatc aaaagttgat gaagtgcaga actaatcaaa ttattcacat
    35221 caatttgttc tttttgaaag actacaatac ctgcatctaa acgtgataat tttaaaaggg
    35281 aagtaatcag ccagtccatt cgtataagta attcctctgt ttctcgtatc aactcttttc
    35341 tctcatcttc atcagaagta ttctctaaca gtgacaaaat aagagttact gatgtaagag
    35401 gagtgcgaag ctggtgggct atgtcagcta gtgagtcagc aaggtgttct ttttcttttt
    35461 tcaatgcgta attttgctct ctaatacgta acgtcatttt agttatttca ctttgcagaa
    35521 tagaaagttc accctcatct gattcagcaa tatacagatg gttagcatta tgaagcacaa
    35581 gattaatttg ttctgaaatc tgtgcaatac ttttgtatcg atttttagta aacacaaaaa
    35641 atgctattcc aaaggtagtg gcagaaacaa tagaaagtat tccagtcata atactgattg
    35701 caaatcccag ggctacagtg agaatagtaa ttaataagaa taaaatagca aattttcgaa
    35761 actctctatt tcgaaacata acttcccccc aatctatacc ctgtcccacg aacagtcaaa
    35821 attatttgtg gacttgaagg gtcatcttct atcttctctc gcaagcgttt gatgtacaca
    35881 gtcaatgtgt tgtcattaac aaactcacct gccgcatccc acaattcatc aagtaatctt
    35941 accctcgtaa taatactttt aggattgcta ataaacacca ataacaaacg gtattctaaa
    36001 gctgaaagaa aaacctcatt gccattcttt ttcacaacac cggttgccgt atctacataa
    36061 agcccacaaa tttcaaaagt tgacccaaaa cgcccacttt ttcgtagtgc tgttctaatt
    36121 cgcgcaatca gttcacgtgg acgaaaaggt ttagtaatat aatcatctgc acccatattc
    36181 agtccagtaa caacacttga ttcatcaccg gaagctgtca aaaagataac aggaatgtct
    36241 tgcttttctt tgatttctgt gcaaatcgta aacccatttc catcaggtaa agaaatatca
    36301 ataagtgcca aatcaaattt attcttagca atctctacaa gtgcttcact tcttgtggat
    36361 acatgggtta ctataaatcc ttctgaatta agcaaaagaa taagattttt ggcaattgct
    36421 ttatcatcct caactaaaaa tatccgtttc atttactttc atcctccttt attttattag
    36481 ttttatatat tccagcagag caatagtaca ctactatttt acttatcatt acacaatttt
    36541 cagtagtttc ttttgttctt atataatcca tgcataatac ctccattgat ttattatatt
    36601 tcttataaca taaatacaca agataagaca aaaaacataa tgtaagtgca agttttttaa
    36661 ctcactaact tataaacata cactcctact aacactactt tcttgatgac acatcttaca
    36721 agcttatatt catacatcaa tgaaaaatta aatttatcca tttattaaaa taaaaggcaa
    36781 tagcttctat agctaatgcc ctttatctta aaatgattta aatactatac aatttttata
    36841 aatggctata aataaactcg tatagaatat aaaatttatc tacagtcttt tgttatacaa
    36901 aatttataac ctcttataga acaaatttac cactttgcaa acttatcact tttactcagc
    36961 tatattataa acttttaatt tatctttgtt taaatgtttc ttcttaactt ctactaaagc
    37021 ttttagcgta tccaaagatg tcattatttc agtagaaaat tcaatggctg ttcttctcat
    37081 cttaaatcca tctcttgtag agtcatttcc tttagttggt gtatttatta ctatatcaac
    37141 ttgtttattt cttatagcat ctaatatgtt tggtctaggc tcttcaattc tatttactat
    37201 atcagcctct attccatttt ctcttaaact cttcgctgtt ccttctgtag caacaaaagt
    37261 atatcctaat tctttcatat cttttgctat ctctataaac tcatctttat catgattatt
    37321 tacagtagca agtactactc ctctttcatc agacatatgt ctacctgctg ccaagaaccc
    37381 tttatataag gcttcttcta aattttctcc tacacctaga acttctcctg tagacttcat
    37441 ttcaggtcct agacttactt caactttaga taatttagac attgagaata ctggtacttt
    37501 tactgatact aattttggct ctttgtaaac tcctgtacta tatcctaaat cctttaattt
    37561 agctcctaac atacacttag tagccaagtc aactattggt actccactta ctttacttat
    37621 atatggaact gttctactag ctctagggtt aacctcaatt atatataatt cattttgaaa
    37681 ttctataaat tgtatattaa ccataccaag cacattaagg tctaaagcta tttttttagt
    37741 gtattctact attttttctt ttatttcatc agatatattt tgacttggat acattgttgt
    37801 actatctcca gagtgaactc cagctctttc taaatgctcc attataccag gtatcaatat
    37861 atcttcttta tcacatatag catctacttc tatttctcta ccagttagat atttatctat
    37921 aagtactgga tttttatggt ctctatcaaa tgcatcatcc aaatactgaa ttagtttttc
    37981 ttcgttataa gttatttcca taccttgacc accaagtaca taagatggtc taactaatac
    38041 tggataacct aactccttag caatttcaac accttcatta gttgtccata caccttttcc
    38101 ttttggtcta tttatatcta gtctctctag taaatcatca aatttttctc tatcttctgc
    38161 tgcatctata tctctaaagt ctgttcccag tataggtata tttttttcat gtaaaaactt
    38221 agccaatttt atagctgttt gtccaccaaa ctgtagtatt acaccttctg gtttttcttt
    38281 ttctattata tttaaaactt cttcttctgt taatggttca aaatacaact tatcagatgt
    38341 atcaaaatcc gtacttacag tttcagggtt attgtttaca attatagttt ctattcccat
    38401 ttttcttaaa gattttacac agtgaactga acaatagtca aattctattc cttgacctat
    38461 tcttattgga ccagaaccca atactacaac ttttctctta tcactaacta ctacctcatc
    38521 atattgttca taagttgaat agtaataagg tgatagagct tcaaattctc ctccacatgt
    38581 atcaaccatt ttataaactg gttgtatatt atataaactt ctcaattcat ataatttttc
    38641 tgggcttatc ttcattaagt cagatatacc tttatcagaa aatccctttt tctttaattc
    38701 atgtaaatat tctttatcta agtcttctat ttttagaact tttaactttt cttcttgttc
    38761 tactatccat ttaaatttat ttataaacca cttatcaaca ccagttattt ctgatatcat
    38821 ttctacttta tagcctcttc ttatcatttc agctaagtca aatagtctct catcatcagg
    38881 tactactact ctagccttta attcttctaa tgttctacct tctgatggag catgaaccaa
    38941 tgaatatttt cctgtttcaa gtgatcttat acctttaaga atagcagcct caaagttact
    39001 tcctatactc atgatttcac cagttgccat catcttagtt cctaattttc tattggcatg
    39061 tctaaattta tcaaatggcc actttggtat ttttactacc acataatcta atgttggctc
    39121 aaaacaagca taagtttttt tagttactgc attttctatc tcatccaaag tataacctaa
    39181 agctatctta gctgctactt ttgctattgg gtatcctgta gcttttgatg ctagggctga
    39241 tgacctacta actcttggat ttatttctat tatcgcatat tctaaactat gtgggtttaa
    39301 agctatttga acgttacatc caccttgaac ctctattgca tttattatgt ctattgatgc
    39361 ctttctaagt agttgatact cttcatcact tagagtttga cttggagcaa caactatact
    39421 gtctcccgta tgaactccaa ctgggtctac attttccata ttacatacag ttatacagtt
    39481 tccatttcca tccctcatta cttcgtattc tatttctttc caacctttta tactcttttc
    39541 aagaagaact tgtccaacag gacttagctg tagtccatgt gaaagtattt ctcttaattc
    39601 ttcctcagta tcagctatac ctccaccagt tccacctagc gtatatgctg gtcttacaac
    39661 tactggatat cctatcttat tggcaaactc aagacctgct tctaaatttg taactatatc
    39721 actaactatt actggttgat ttatttcttt cattacttct ctaaataaat ctctatcttc
    39781 gccctttttt atagattcta tagatgttcc tataactttt acattatatt tatctaatat
    39841 accagcatca tgtaattcta cagctaagtt taatcctgtc tgaccaccca tacctgcaag
    39901 taaactatct ggtttttctt tttctattat tttttctata aattctattg ttaatggttc
    39961 tatatatatt ttatctgcaa tttctttatc agtcattata gtagctgggt tactatttac
    40021 taatacaact tcaattccct cttcttttaa tgcttggcag gcctgtgttc ctgaatagtc
    40081 aaactctgca gcttgaccta ttattattgg ccctgaccct aatactaaag ttttctttat
    40141 actatctaat ttaggcataa ctctttctcc ttatctaaac aattttcaca gttcttatct
    40201 tcatcatcta tacaatcaac tttaattact ttattttatc ttaaattcaa atttactcat
    40261 caatacatct atttaataca tctatgttaa ttaaaccaaa ctttttgatt taagttattc
    40321 ttgtttttta gttagctatt ctaatgttat ttattttact aattctaaaa atttatcaaa
    40381 aatatagtca ttatcttttg gtcctgggca agcttcagga tggtattgaa cactgtatac
    40441 atctagtttc tcatgtctca ttccttcaac agtattatca tttaaattta tatgtgtcac
    40501 tttcatttgt tcaggaactt ctgaaacata ataaccatga ttttgagaag ttataaatac
    40561 ttttccttct tctaaatctt taactggatg atttccacct ctatgcccaa atttaagttt
    40621 agcagttttt cctccaagag ctaaagctaa aagttgatgt cctaagcata ttccaactat
    40681 aggtttttta ccaataagag cttttatatt ttctataaca tcttctaggt cttctgggtc
    40741 tccaggtcca tttgacaaga atattaagtc agggtttata ctcaatacat cttctggaga
    40801 tgttgtagct ggaaatattg ttatatcaca tccacgagct ataaatgatc ttaatatatt
    40861 tctttttact ccaaaatcca taacagccac ttttggacca tttcccttta tatgctctac
    40921 ttcttttctt gtaacagttc taactgcttc tgtatttgaa aacttatcca atttatgttt
    40981 aacatcttct aatttactgt cttctaatgt aattatacct ttcatggttc cattatttct
    41041 aagtatctta gtcaaagctc ttgtatctat tccttcaagt ccgataactt tattttgctt
    41101 taaatattgg tctatatcaa tttcacatct aaaattactt ggagaatcac ttttctccct
    41161 tactatgaat ccctttactt gaactttttc agactctgct gatgataaat ttataccata
    41221 atttcctaca agaggataag tcatagtaac tatttgacca tagtaagaag ggtctgttaa
    41281 aacttcacca tatcctatca ttgaagtatt aaatactact tctcctacac tttcttctaa
    41341 atatccaaat gcctttccta taaaaactgt tccatcttct aatattaacc ttgctttcat
    41401 actacagtac ctcctataat tctctttaga caaactaaag aacattaact aaatataagc
    41461 ttttaaatat ctatatctat ttttcacttt tacattctag ttttatactt atttacattt
    41521 attttttatc tttttctatt atactacaaa tttcttaaaa aaaagaagtt atctcaaaag
    41581 taaaaactcc tagaaaaaat aacctctttt ttttatttaa taattaaatt cttaaactat
    41641 acaatcaact taatttctat tcagctatat tgtaaacttt taactcatct ttatttaaat
    41701 gtttcttctt aacttctact aaagctttta gtgtatccaa agatgtcatt atttcagtag
    41761 aaaattcaat agctgttctt cttatcttaa atccatctct tgtagagtca tttcctttag
    41821 ttggtgtatt tattactata tcaacttgtt tatttcttat agcatctaat atgtttggtc
    41881 tagattcttc tactctattt actatatcag cctctattcc attttctctt aagctattag
    41941 ctgttccttc tgtagcaaca aaagtatatc ctaattcttt catatctttt gctatctcta
    42001 taaactcatc tttatcatga ttatttacag tagcaagtac tactcctctt tcatcagaca
    42061 tatgtctacc tgctgccaag aaccctttat ataaggcttc ttctaaattt tctcctacac
    42121 ctaggacttc tcctgtagac ttcatttcag gtcctagact tacttcaact ttagataatt
    42181 tagacattga gaatactggt acttttactg atactaattt tggctctttg taaactcctg
    42241 taccatatcc taaatccttt aatttagctc ctaacataca cttagtagcc aagtcaacta
    42301 ttggtactcc acttacttta cttatatacg gaactgttct actagctcta gggttaacct
    42361 caattatata taattcattt tgaaattcta taaactgtat attaaccata ccaagcacat
    42421 taaggtctaa agctattttt ttagtgtatt ctactatctt ttcttttatt tcatcagata
    42481 tattttgact tggatacatt gttgtactat ctccagagtg aactccagct ctttctaaat
    42541 gttccattat accaggtatc aatatatctt ccttatcaca tatagcatct acttctattt
    42601 ctctaccagt tagatattta tctataagta ctggattttt atggtctcta tcaaatgcat
    42661 cttgtaggta ttgagataat ttttcttcgt tataagttat ttccatacct tgaccaccaa
    42721 gtacataaga tggtctaact aatactggat aacctaactc attagcaact tcaatacctt
    42781 cgttaagtga ccatacaccc tttccttttg gtctatttat atctagtctc tctagtaaat
    42841 catcaaattt ttctctatct tctgctgcat ctatatcatc aaaatcagtt cccagtatag
    42901 gtatattttt ttcatgtaaa aatttagcca attttatagc tgtttgtcca ccaaactgta
    42961 gtattacacc ttctggtttt tctttttcta ttatacttaa aacttcttct tctgttaatg
    43021 gttcaaaata cagcttatct gatgtatcaa agtctgtact tacagtttca gggttattgt
    43081 ttatgattat agtttctatg tccatttttc ttaaagattt tacacagtga actgaacaat
    43141 agtcaaattc tataccttga cctattctta ttggaccaga acctaatact atcacctttt
    43201 tcttatcact aacaactacc tcatcatatt gctcataagt tgaataatag taaggagata
    43261 tagcatctat ctcacctgca caagtatcaa ccattttata tgctggtttg atattgtata
    43321 gacttctcaa ttcatataat ttttctggac ttatcttcat taggtcagat atacctttat
    43381 cagagaatcc ctttttcttt aattcaagaa gatagtcttt agttaaatct tcaattttca
    43441 taacttttaa tttttcttct tgctctacta tccatttaaa tttatttata aaccatttat
    43501 ctatacctgt aatttgttct atcatttcta ctttatagcc tcttcttatc atttcagcta
    43561 aatcaaatag tctctcatca tcaggtacta caactctttt ctttaattcc tcaatgcttc
    43621 tctcctcaga tggtgtgtga actaatgaat attttcctgt ttcaagtgat cttatccctt
    43681 taagaatagc agcctcaaaa ttacttccta tactcataat ttcaccagtt gccatcatct
    43741 tagttcctaa ttttctattt gcttttttaa atttatcaaa aggccatttt ggtatttttg
    43801 ccaccacata atctatagtt ggttcaaaac aagcataagt ttttttagtt actgcatttt
    43861 ctatctcatc taaagtataa cctaaagcta tttttgatga aactttagct attggatatc
    43921 ctgtagcttt tgatgctaaa gctgatgacc tactaactct tggatttatc tctataactg
    43981 catattcaaa actatgtgga ttaagtgcaa attgaacgtt acaaccacct tgaacttcta
    44041 ctgcatttaa tatatctaaa gatgcttttt taagcattgc acattctttg ttacttaaag
    44101 tttgagttgg agcaacaact atactgtctc ctgtatgaac tccgactggg tctatatttt
    44161 ccatgttaca tacaactata caatttccct ttgaatctct tattacctca tattctattt
    44221 ctttccaacc ttttatactc ttttcaatta atacttgtcc aacaggactt aattgtagac
    44281 catgtgaaag tatttctctt aattcttcct cattatcagc tataccccca ccagttccac
    44341 ctagtgtgta tgctggtctt acaactactg gataaccaat tgtaagtgca taatcaagac
    44401 ctgcttgtaa atctgtaact atatcactaa ctattacagg ctgatttatt tctttcatta
    44461 cttctctaaa tatatctcta tcttcacctt ttttgataga ttctactgaa gtacctatta
    44521 tttttactcc atatttatct agtatgcctt tttcataaag ttcaactgct aggtttagcc
    44581 ctgtttgtcc acccatacct gcaagtaaac tgtctggtct ttctttttct attatttttt
    44641 ctataaattc tattgttaat ggttctatat atattttatc tgccacttct ttatcagtca
    44701 ttatagtggc tggattacta tttattaata caacttcaat cccttcctct ttcaaagact
    44761 ggcaagcttg tgttcctgaa tagtcaaact ctgcagcttg acctattatt attggtcctg
    44821 accctaatac taaagttttc tttatactat ctaatttagg cataaatttt tctccttatc
    44881 taaatggttt ttactatctg taaataatac taaatctata ttaatgctaa gaatttatta
    44941 aatatacaat ctaaatcttc ttttgttgtg tattctggaa tgtactgaac actatatatt
    45001 ggtaattcct tatgtctcat tccttctata gtattgtcat ttaaatttat gtgagttact
    45061 tctgtatttt ctggcatttc tgaaacatta tatccatgat tttgagaagt tataaatacc
    45121 tttccagttt ctaaacattt aactgggtga ttacctcctc tatgtccaaa tacaagcttt
    45181 gaagtctttc ctcctaatgc caatgctaaa atttgatgtc ctaaacctat tcctgtcaat
    45241 ggctttttgc ctattaattt ttttatattt tcaactatat catttatatc ctctggattt
    45301 cctgggccat tagataataa gattaagtca ggatttatat ttaaaatttc ttctactgta
    45361 gtcattgctg gtactatagt tatatcacag ttacaagcct taagagactc tagtacacta
    45421 tttttaacac caaagtctat aactacaact ttagctccag taccttttat attttcaatt
    45481 tcttttcttg taacattttt tactgaatct ttgttacaat gctcttctaa tttagactga
    45541 acatcttgta atgatacatt ttctaaagtt ataattcctc tcttagttcc attgtttcta
    45601 ataattttag ttaatgctct tgtatctatt ccttcaagtc ctattacctt attttgcttt
    45661 agatatgtgt ctatatccat ttcacatcta aaattatttg ggttatcact tttttctctt
    45721 acaataagtc cttttacttg caccttattt gattcaacat cttctaaatt tattccatag
    45781 tttcctataa gtggataagt catagtaact atttgtccat aataagttgc atctgtcaat
    45841 agttctccat acccagccat tgaagtatta aatacaactt ctccaatagc ttcctttaga
    45901 tatccaaatg cttttccttc aaatactgtt ccatcttcta aaattaattt ccccttcatg
    45961 taagctacct cccaaatatt ttatctaagc attttctatt tctcttacaa tattaatagc
    46021 tgattctctt ggattttctg cttttgttat aggtcttcca acaactaaat aatgagcacc
    46081 tttttttatt gcatctgatg gagttacaac tcttttttgg tctccaacct ctgctgattt
    46141 aggtcttatt cctgggcaaa ctgttataaa atcagttcca catgcttcta ctattttatc
    46201 tacttcttgc gctgaacaaa caaccccatc tattcctgat tcttttgcca gtttagctct
    46261 ctttatagct aactcttctg ttgtcatatt acatcctatg tcctgtaaat cttggttgct
    46321 taatgaagtt aaaactgtta ctccaactac taatggcttt tctatattta atctttctgc
    46381 ttcttcccta gcaattagtg cacattgtct cattccttcc atatctgata catgtattgt
    46441 cattaaccaa acattatctc tcactgcagc tttcactgca cttttcatag tatttggtat
    46501 atcatggaat ttaaggtcta aaaatatttt tttacctttt tctttaagat aatctattgt
    46561 tttacctttt gtggcaacgt attgctctag ccctacctta aatatgtcta cactatcttc
    46621 taatctgtct attagttctt tagctttatc aaattcgtca gtatcaattg caactatcaa
    46681 tttttcttta ccatttatca tttttacatc tcctttttaa taaagcctaa ataatcataa
    46741 aaaaatcctc acaccaaaag aggcataagg attttatcta tacataactc tattcattag
    46801 ctatgagata tctttcccag tctctctgga ctagtttaaa agactttatt atatttaaat
    46861 ttttatttta tactcatcaa aaacctctag aagatttact agaggttata tactaacatt
    46921 atctataacc ctttagtctc tctggactac tttaaggaat ttagctgtaa tatttttctt
    46981 tatttatata actttatgta tctataggat aatttaaagt tttatctttt ttataattaa
    47041 accactaatt gctttttatg tcaataagtc tttgaattat tcttcatctt tatcaaacaa
    47101 ttttttctca aaatatgatt ctgcattata tattgcagca actggattat gcccagttac
    47161 tctatctttg acaactaaag ttgttatagg agcttccata tatttcatgg ctaaagtatc
    47221 gtgaccaaca cataatccaa gaattacatt aaggtcaacc ttagcttcat ttaaaaataa
    47281 agcttgtcct attggattgc acattacctc atctgaacac tttgatattg tttgagatgc
    47341 ttctagtcca attgtactct taggcattgc cccattttta catataacag aaaatacttc
    47401 aaacccatga tgttcaaata ttttttgcac tgtttgcatt tctcttttta gtccaataca
    47461 aaatgctatg ccaatctttt tataaccaca ctttttagca aactctataa tctcttttag
    47521 tctagtatat tgacaatacc cctctgcttc aactaatcca gcattataag ctatttttct
    47581 attctcttct tcttcataaa gttgctttgc tttttgttgt agttcaattt ctctagttgg
    47641 acatatcttc attaatttat ccatatttcc attagaacag cctctaagat tacattttcc
    47701 acacttatac atatccaata tccccttcca aaattttatt catcttgcga ttcttctttt
    47761 tctagagtta cattttcttc ttgggtttct tcttctggtt tcacatgctc gactacagtt
    47821 ataaaatatg attttcctat tttattcctt ataggaagtt tttcatatga aacatctatt
    47881 ttatttttta tacaagctat atcaaaatgt cttaaggcta cacctaagct aaccttattc
    47941 atatcttcac agtgtttctt aggtctcttt tcaaataaat gaagacctcg ttcatcctta
    48001 tggaaatacc acggttgaga attttttaca gatggagctc ttcttgtcaa ttctaagata
    48061 tctttccact ttctattcct atttatctta tttatcatat cttttactgg ctttctatca
    48121 ggtctagcat atctcgtcct aaacaactct tcattttttg ctggatatcc aaatgctatt
    48181 attatataag gattttctat tttgttttca tcatcttctt ctgtttttat tccagctaga
    48241 taatctaaat catcttcctc atcttcttca aaattcttat tcccaatatc tacaaattcc
    48301 aagatatctt ctctctttaa gtttccttcc aaccagcaag tagctattcc caatgttgta
    48361 aggcgcaaaa ctacaccttc tgtagcaaat cctatattct gtaaatagtc ctcacctttg
    48421 tctgatgtaa ctactatata atgaggtgcc tttactttac attccttacc catcaaaaaa
    48481 tgtattatat gacctctatc aacaacatgt gctttaatat ttaaatcttc atttaagtat
    48541 tctaaatttt tacaacttct ttttatttct tccatcaatg gcttttttat cttcttactg
    48601 gaaaaatttc ttacagattt tctataaaaa atggcttcat aaagctccat ttatgccctc
    48661 ctaactgaaa tcttctgacg tataaactct ataaatttat ataaatactt tatatttttt
    48721 atagttttat ttcctttttc ctcctgccct attttgaaat tcataagaat atctactcta
    48781 gtttaatata gcattcaaaa ggcttaaatt ctaccttatc aatgtctata cattagcata
    48841 atttttttat ttgattttct atgctaattt aaataacaac actagctatt acctttttaa
    48901 ttaagctttg ccagaaaaat tttttttcat ttataaatat ctatctcatt attatattat
    48961 aagttttttt cggtattctt gtatagcttt tcactaaaaa agtattttta tttctacttt
    49021 aatattatta tcaacaaaa
//
LOCUS       DAMKMI010000012        48855 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMKMI010000012 DAMKMI010000000
VERSION     DAMKMI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.012"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            23..1219
                     /locus_tag="PC429_000675"
     CDS             23..1219
                     /locus_tag="PC429_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF3846193.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1312..1836
                     /locus_tag="PC429_000676"
     CDS             1312..1836
                     /locus_tag="PC429_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF3846194.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1861..2037
                     /gene="rpmF"
                     /locus_tag="PC429_000677"
     CDS             1861..2037
                     /gene="rpmF"
                     /locus_tag="PC429_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF3846195.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2244..2801
                     /gene="fapR"
                     /locus_tag="PC429_000678"
     CDS             2244..2801
                     /gene="fapR"
                     /locus_tag="PC429_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF3846196.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2812..3834
                     /gene="plsX"
                     /locus_tag="PC429_000679"
     CDS             2812..3834
                     /gene="plsX"
                     /locus_tag="PC429_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF3846197.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3860..4846
                     /locus_tag="PC429_000680"
     CDS             3860..4846
                     /locus_tag="PC429_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF3846198.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            4955..5884
                     /gene="fabK"
                     /locus_tag="PC429_000681"
     CDS             4955..5884
                     /gene="fabK"
                     /locus_tag="PC429_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF3846199.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5900..6850
                     /gene="fabD"
                     /locus_tag="PC429_000682"
     CDS             5900..6850
                     /gene="fabD"
                     /locus_tag="PC429_000682"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF3846200.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6850..7599
                     /gene="fabG"
                     /locus_tag="PC429_000683"
     CDS             6850..7599
                     /gene="fabG"
                     /locus_tag="PC429_000683"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF3846201.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7653..7877
                     /gene="acpP"
                     /locus_tag="PC429_000684"
     CDS             7653..7877
                     /gene="acpP"
                     /locus_tag="PC429_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF3846202.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7906..9144
                     /gene="fabF"
                     /locus_tag="PC429_000685"
     CDS             7906..9144
                     /gene="fabF"
                     /locus_tag="PC429_000685"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF3846203.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9370..11088
                     /locus_tag="PC429_000686"
     CDS             9370..11088
                     /locus_tag="PC429_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3846204.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKHDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11119..13119
                     /locus_tag="PC429_000687"
     CDS             11119..13119
                     /locus_tag="PC429_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3846205.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSELSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13470..13715
                     /locus_tag="PC429_000688"
     CDS             13470..13715
                     /locus_tag="PC429_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF3846206.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13750..14475
                     /locus_tag="PC429_000689"
     CDS             13750..14475
                     /locus_tag="PC429_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF3846207.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14497..15810
                     /locus_tag="PC429_000690"
     CDS             14497..15810
                     /locus_tag="PC429_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3846208.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYDENLKKIV"
     gene            16016..16555
                     /locus_tag="PC429_000691"
     CDS             16016..16555
                     /locus_tag="PC429_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3846209.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16644..18629
                     /locus_tag="PC429_000692"
     CDS             16644..18629
                     /locus_tag="PC429_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF3846210.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18853..19821
                     /gene="spoIIIAA"
                     /locus_tag="PC429_000693"
     CDS             18853..19821
                     /gene="spoIIIAA"
                     /locus_tag="PC429_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF3846211.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19811..20332
                     /locus_tag="PC429_000694"
     CDS             19811..20332
                     /locus_tag="PC429_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF3846212.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20349..20546
                     /gene="spoIIIAC"
                     /locus_tag="PC429_000695"
     CDS             20349..20546
                     /gene="spoIIIAC"
                     /locus_tag="PC429_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF3846213.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20562..20948
                     /gene="spoIIIAD"
                     /locus_tag="PC429_000696"
     CDS             20562..20948
                     /gene="spoIIIAD"
                     /locus_tag="PC429_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF3846214.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21033..22238
                     /gene="spoIIIAE"
                     /locus_tag="PC429_000697"
     CDS             21033..22238
                     /gene="spoIIIAE"
                     /locus_tag="PC429_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF3846215.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22298..22915
                     /locus_tag="PC429_000698"
     CDS             22298..22915
                     /locus_tag="PC429_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF3846216.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22927..23481
                     /locus_tag="PC429_000699"
     CDS             22927..23481
                     /locus_tag="PC429_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF3846217.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23508..24197
                     /locus_tag="PC429_000700"
     CDS             23508..24197
                     /locus_tag="PC429_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF3846218.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSYSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24307..24669
                     /locus_tag="PC429_000701"
     CDS             24307..24669
                     /locus_tag="PC429_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF3846219.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24754..25260
                     /gene="nusB"
                     /locus_tag="PC429_000702"
     CDS             24754..25260
                     /gene="nusB"
                     /locus_tag="PC429_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF3846220.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25244..26314
                     /locus_tag="PC429_000703"
     CDS             25244..26314
                     /locus_tag="PC429_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF3846221.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26311..27516
                     /locus_tag="PC429_000704"
     CDS             26311..27516
                     /locus_tag="PC429_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF3846222.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27518..27733
                     /gene="xseB"
                     /locus_tag="PC429_000705"
     CDS             27518..27733
                     /gene="xseB"
                     /locus_tag="PC429_000705"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF3846223.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27736..28623
                     /locus_tag="PC429_000706"
     CDS             27736..28623
                     /locus_tag="PC429_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF3846224.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28653..29090
                     /locus_tag="PC429_000707"
     CDS             28653..29090
                     /locus_tag="PC429_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF3846225.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29124..30989
                     /locus_tag="PC429_000708"
     CDS             29124..30989
                     /locus_tag="PC429_000708"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF3846226.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31122..31934
                     /locus_tag="PC429_000709"
     CDS             31122..31934
                     /locus_tag="PC429_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF3846227.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            31954..33633
                     /gene="recN"
                     /locus_tag="PC429_000710"
     CDS             31954..33633
                     /gene="recN"
                     /locus_tag="PC429_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF3846228.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33848..34714
                     /locus_tag="PC429_000711"
     CDS             33848..34714
                     /locus_tag="PC429_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846229.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34807..35298)
                     /locus_tag="PC429_000712"
     CDS             complement(34807..35298)
                     /locus_tag="PC429_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3846230.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35359..35799)
                     /locus_tag="PC429_000713"
     CDS             complement(35359..35799)
                     /locus_tag="PC429_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3846231.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            35960..37018
                     /locus_tag="PC429_000714"
     CDS             35960..37018
                     /locus_tag="PC429_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF3846232.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGGAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37160..37984
                     /gene="spo0A"
                     /locus_tag="PC429_000715"
     CDS             37160..37984
                     /gene="spo0A"
                     /locus_tag="PC429_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF3846233.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38397..39518
                     /locus_tag="PC429_000716"
     CDS             38397..39518
                     /locus_tag="PC429_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846234.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39536..40411
                     /locus_tag="PC429_000717"
     CDS             39536..40411
                     /locus_tag="PC429_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF3846235.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40435..41127
                     /locus_tag="PC429_000718"
     CDS             40435..41127
                     /locus_tag="PC429_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3846236.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41135..41959
                     /locus_tag="PC429_000719"
     CDS             41135..41959
                     /locus_tag="PC429_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF3846237.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            41964..43031
                     /locus_tag="PC429_000720"
     CDS             41964..43031
                     /locus_tag="PC429_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF3846238.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43033..43569
                     /locus_tag="PC429_000721"
     CDS             43033..43569
                     /locus_tag="PC429_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF3846239.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43637..44242
                     /locus_tag="PC429_000722"
     CDS             43637..44242
                     /locus_tag="PC429_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF3846240.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44254..45168
                     /locus_tag="PC429_000723"
     CDS             44254..45168
                     /locus_tag="PC429_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3846241.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45210..46382
                     /locus_tag="PC429_000724"
     CDS             45210..46382
                     /locus_tag="PC429_000724"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF3846242.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46429..47241
                     /locus_tag="PC429_000725"
     CDS             46429..47241
                     /locus_tag="PC429_000725"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF3846243.1"
                     /translation="MFEKIAQSSKFINSKSDIKPKIGLILGSGLGDLANDIEDPVAIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47445..48770
                     /locus_tag="PC429_000726"
     CDS             47445..48770
                     /locus_tag="PC429_000726"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF3846244.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
BASE COUNT        19748 a         4932 c         9183 g        14992 t
ORIGIN      
        1 aaaatctaag gaggaaataa taatgaaaat attagtatta aactgtggga gttcttcatt
       61 aaaatatcaa ttaatagata tgaataatga agaagtttta tgtataggat tggttgaaag
      121 aataggaata gaaggttcta tattaaaaca tgaaaaagca ggtagagatg ataaatacgt
      181 tgttgaacaa ccaatgaagg accacaaaga tgcaatagca ttagttttag aagctgttgc
      241 tcatccagaa tttggtgcag ttaaagaaat gaaagaaata gatgcagtag gacatagagt
      301 tgtacatgct ggagaaaaat ttgcaacttc agtagtaata actccagaag tagaagaagc
      361 tttaaaagaa tgtatagatt tagctccact tcataatcca gcaaacataa tgggaataga
      421 tgcttgtaaa gctatacttc cagatgtacc aatggtaggt gtatttgata ctgctttcca
      481 tcaaacaatg cctaaatctt catacttata tggtttacct catgagctat atactaaata
      541 tggggtaaga agatatggat tccacggaac ttctcataat tatgtatctc agagagcagc
      601 agagatatta ggaaaagata taaaagattt aaaaatagta acttgtcact taggaaatgg
      661 tgcttctata gctgctgttg atggtggaaa atgtgtagat acatctatgg gattcactcc
      721 attagaagga ttaataatgg gaactagatg tggagatata gacccagcta tattaccatt
      781 cttaatgaga aaagaaggtt tagatgctga tggattggat aaattaatga ataaagaatc
      841 tggagtatat ggaatgactg gaatttctag tgacttcaga gatatagaag atgcagctaa
      901 aaatggagac gaaagagctc aagctacatt agaagcgtat gttaaaaaag tacaaaaata
      961 tataggcgct tatgctgctg aaatgaatgg attggatgtt gtagtattta ctgcaggcgt
     1021 tggtgaaaat ggaaaggcta taagagcaga tattgcttct aacatggagt ttttaggaat
     1081 gaaattagat aaagaagcta atgatgttag aggaaaagaa acagtaatat ctactgctga
     1141 ttctaaagta aaaatgcttt taataccaac taatgaagaa ttaatgatag ctagagatac
     1201 attaagattg gtaaaataat taaattaaac ctatgcctat caagtaaaaa tacttgacaa
     1261 acaaatgcta gatttgtata ataaatttgg tgatatgttg aggtgaactt aatgaaagtt
     1321 agcatagaaa aaataaatag aaaagaaact gacaaaatag acttgaattt ttgtgaaaaa
     1381 attgatacta ttagttattg tgatgagatt tacaaattag tatcacctgt aaatttaaaa
     1441 gggaaagtgt caaagactaa taaaggtttg tatctagata tagatgtcaa ttttacaatt
     1501 gttgacaatt gttcaagatg tcttaaagaa gtagaaatac cactagagta ttctatacaa
     1561 ggttttttag taaaagaaga agattatgat gaagatgagt ttgaggaatt tgatcctttt
     1621 atattcgatg gtgaagaaat cgaccttatt gatataatag aacagacatt agattttaat
     1681 gtacctcata aagttctttg tagtgaaaac tgtaaaggtc tttgtcaagt atgcggggca
     1741 aacttaaatg aagaagagtg ttcttgcagt gaaattacaa acgatgagga atacatagat
     1801 cctcgttttg ctaaattaaa agatttattt aattaaattt tgactaagga ggtggcgtaa
     1861 atggcagtac caaagcgtaa aacgtctaaa tcaaacacaa aaatgagaag agcagctaac
     1921 tcaaagatgg aagcaacagg atttgtaagt tgcccacaat gtcatgagcc aaaattacca
     1981 catagagtgt gtccagactg tggatattat aaaggtaaag aagttgtgtc taagtaatag
     2041 cttctaaaaa gcatgtagag tatactctac atgttttttt tgtacatata aaaaaataag
     2101 atgatttact agagaaaata agtggatttt atgcatatta aaaaatagaa aaataataat
     2161 ataagtattg aaaatttttg tgtgttacta tatactacta ttagtagctg gtactaatta
     2221 cagctataaa atttggaggg aacatgaaaa agaaaagtaa agcccaaaga caaaaagagc
     2281 ttatagatat gctaaagaca gatccttttt atactgatga agaattatca agtctgtttg
     2341 atgtaagtat acagaccata agattagata gaatgagtct aaacatacca gagctaagag
     2401 aaagagtcaa atcaatagca gaaactcaga gttcaaaagt aaagacttta ggagtaaagg
     2461 aaattacagg agaaatcatt gatttatctg tagggcgatt aggtatatct atgttagaag
     2521 ttacacagga catgatttac tctaagacta atactttaaa agatacgtat attttttctc
     2581 tagcagattc attggcaatg gctataatag atgcacctaa ggttattatg agagttgcaa
     2641 atgtaaaaag ttttaagttg atagaacaac aagatagact tatagcaaaa gcagaagtgt
     2701 atagaaatat agataaaaag cattatgtaa aagttgttat caacaataaa gcccaagagc
     2761 aaatatttag aggaaagttt atctttgaag aattagatta ggagggaaat tatgaaaata
     2821 gtaattgatg gaatgggtgg agataatgca cctaaatcta atgtagaagg cgcagtaaat
     2881 gctataaaag aatatcaggt tgatttaata atcacaggag ataaagattt acttgaaaag
     2941 gaattttcaa attatgaatt tgatagaaat aaattagaga tagttcatac tactgagatt
     3001 atagaaaacg aagataaacc agtaaaggct ataagaagta aaaaagattc atctatggta
     3061 gtagcattaa atcttgtaaa agaaggaaag gcagatgcta taatatcagc tggaaataca
     3121 ggagccttac ttgctggtgg attatttgtt gttggtagaa taaaaggaat cgatagacct
     3181 tgcttatgtt cagcaatacc aaatgtaaag cgaggaatga cacttatagc agactgtggc
     3241 gcaaatgcag attgtaaacc taagaatcta gttgaatttg cagctatgag taatatttat
     3301 tcaagaaaag ttttaggtct tgaaaatcct aaggtagcat tagcaaatgt tggactagaa
     3361 gaaggcaaag gcaatgattt agtcaaaagg tcatatgaag aaattaaaaa attagattta
     3421 aattttatag ggaatgtaga agctagagaa gttataaatg catacacaga tataatcata
     3481 tgtgatggat ttacaggtaa tatattactt aaatctgctg aaggtgtagc actttcagtt
     3541 atgagtctta taaaggaaac ttttatggcc agtacaaaaa gtaaaatagg agcattactt
     3601 ataaaggatg atttaagaaa attaaaaagt tttatagatt attctgagta tgggggagct
     3661 ccacttttag gtctaaatgg aggagttata aaagctcatg gtagttctga tgctaaagct
     3721 ataaaaaatg ctataaatca aggaataaaa ttttcaaagg gaaaagtcgt tgaagatatt
     3781 aaccaattta taagcaaata caatgaagaa aataaaaata acgaagatga ataaactcgt
     3841 tttggaggtt taatatataa tgaatacaaa ggctggaata ttaggagtag gaagttatct
     3901 acctgagcaa tcatatgata attttcactt cgaaaaaata atggatacat cagatgagtg
     3961 gataagtact agaactggta ttaaggaaag aagatttgcc aaagagtctg aagcaacttc
     4021 tgacttagca tctaaagctg ccttaaaagc tatagaatgt gcaaagttaa acgtagagga
     4081 tatagagctg attatattag ctacaattac tcctgatatg tctttgcctt caactgcatg
     4141 tatagtacaa gatgcaatag gagcagtaaa tgctactgca tttgatatat cagcagcttg
     4201 ttctggattt gtatatggag taactattgc aaagcaattt gtggaaacag gttgctataa
     4261 aaatgtgctt gtaataggag cagaaacttg ttctaagttt ttaaattatg atgatagaac
     4321 aacagcagta ttgtttggag atggagctgg agcagctgtt attggccctg taaatgaggg
     4381 tggaatactg tctacacata tgggctctga tggtaaagga aaagattgtt taaaagtacc
     4441 tgctggaggt tcaagactta aggcaagtaa agagactgta gaagctaatc tacatacaat
     4501 agaaatggca ggtagtgatg tgtttaaatt tgcagttaga aagatggcag aaacttcttt
     4561 aagagctctt gaaaaagcaa atctaaatac aactgatata gactacttag tgccacatca
     4621 agctaatata agaatcatac aagcttcttc taagagatta gagcttgata tgaaaaaagt
     4681 ttatgtaaat atagacaaat atggaaatat gtctgctgca tctataccag tagcattaga
     4741 tgaagcttat agagaaggta aaattaaaaa gggtgataat gtagttttag ttggctttgg
     4801 tggaggtcta acttgggggg cttcagttgt caagtggact ttatagtata aaatatctaa
     4861 aatgatttaa ttaacaaaat taaacttata cacaaaataa agttgattaa taataaaaag
     4921 tttactttta tgatttcaag tgaaggagaa ttgtatgaat aaaatttgca aaatattaaa
     4981 tataaaatac ccagttatcc aagggggaat ggcatgggta gctactgcat cattagcaag
     5041 tgctgtatct aatgcaggag gacttggcat aatagcagca ggaaacgcac caaaagaagc
     5101 tataaagaaa gaaattgttg agtgtaaaaa attaacagat aaaccttttg gagtaaatgt
     5161 aatgcttatg tcgccatttg ttgatgatat aattgatttg attatagaag aaaaagttca
     5221 agttattact actggtgctg gaaatcctgc aaagtatatg gatagattaa aggaagctgg
     5281 aacaaaggtt attcctgtag tacctacaat agctttggca caaagaatgg aaaagctagg
     5341 agctacagca gtaatagcag aaggtactga aggtggagga catataggag aacttactac
     5401 tatggtctta gttccacaag ttgctgatgc tgtaaacata cctgtaatag ctgctggagg
     5461 aattgtagat ggtagaggaa ttgcagcatc atttgcatta ggtgccagtg cagttcaagt
     5521 aggaactaga tttatttgca gtgaagagtg ttctgtccat tcaaactata aaaacttagt
     5581 actaaaagca aaagatagag atgcaattgt aacaggaaga agtactggtc atccagtaag
     5641 aacattaaaa aataaactat caaaagaatt tttaaagatg gaacaaaatg gagctactcc
     5701 tgaagaactg gataaaaaag gtacaggagc tttaagattt gcaacagtag atggagacat
     5761 agaaaaaggt tcatttatgg caggtcaaag tgctgctatg gtaaaagaaa taacaccttg
     5821 taaggaaatt atagaggcta tggtaaatca agcaagagag attatgccag caatagaact
     5881 gtaaatcagg aggagcaaga tgggaaaagt tgcactagta ttccctggtc aaggagctca
     5941 atatgtaggt atggccaagg atttatatga aaataatact gttgctaaaa gtgtaataga
     6001 tgaggctagt gatgccttaa atatggattt aaaaaacctt atgtttaatg gaaatgaaga
     6061 agaactttct aaaactgaaa acactcaacc tgctatggtt acacatagtg tagctgtatt
     6121 aaaagcagta caagcccaag tagatttaaa atatagtgca tgtttaggtt tatctttagg
     6181 agagtatagt gcattggttg cagcagatgc aattgacttt aaagatgctg tatgcttggt
     6241 caaaaaaaga ggtaagttta tgcaggatac agttccaaca ggtgttggag ctatggcagc
     6301 aattttaggt ctagatagaa gtatattaga aaatgtagtt aaagatatac aaggaggtat
     6361 tgtggaagta gcaaattata attctcctgg tcaaatagtt atatctggtg aaaatgaaaa
     6421 gatagaagaa gcaatggtga aatgcaaaga agcaggagca aaaagagctg ttaaattaaa
     6481 tgtaagtgga ccttttcact catcaatgct aagagaagct ggtgtaaaat tggccagtga
     6541 attagaaaaa gttaacataa ctaaacctaa agttgatgtg gtggcaaatg taaatgcaga
     6601 ttactataaa aacgaaggta aagacctgct tataaaacaa gtaagttcat ctgttctttg
     6661 ggaagattct atagaaagac ttttaaatga tggatatgat acttttatag aaatgggacc
     6721 tggaaaaact ttaaaagcat ttattaagaa aatagcttcc aataaaaaag caaatgtcaa
     6781 tatttataat atagatggta tagattctct taatgaattt gtacaaaatt atagaaatgg
     6841 agagatataa tgataaatct tacaggacaa gtagcagttg ttactggtgg ctctaggggt
     6901 ataggaaaag aaatagcaaa aaaactagca tcttttggag ctgatgtagt aatcaattat
     6961 acttctaaag aagatgaagc actaaaaact aaaaatgaaa tagaaagtat gggggtaaag
     7021 tgtacctcta taaaatgtga tgtgtctaaa tttgatgaag tgaatcaaat gatagattct
     7081 gttgtaagcg aatttggaaa aattgatata ttggttaata atgcaggcat aactaaagat
     7141 ggtctgctta tgagaatgaa agaagaagat tttgatagag ttatagatat aaacttaaaa
     7201 ggtgtcttta attgtacaaa agcagttact aaacctatga tgaaaaagaa gtatggaaga
     7261 ataataaata tgacttcagt agttggaatt atgggtaatg cagggcaaac taattattgt
     7321 gcatcaaaag caggtgtaat tggatttaca aaagcttctg caagagagtt agcatcaaga
     7381 aacataaata taaatgcagt agcacctgga tttatagaaa cagatatgac taaagtacta
     7441 agtgatgatg taaaagaatc aacactagca aacataccaa agaaatctta tggtaaacca
     7501 gaagatgtag ccaatgccgt agcattttta gttagtgaca tgtcaagtta tataacagga
     7561 caagtaataa atgtagatgg tggaatggta atgcaataat aatttaaaaa gtagagaaat
     7621 ctattaaaat aaaaaatttt aggaggtaca caatgtttaa taaagttgta gaaataataa
     7681 tagagcaatt aggagtagaa gataaagaaa taactatgga aacatcatta atgaaggatt
     7741 tagaagcaga ttcattagat gcagtagaaa taataatggc acttgaagat gaatttggta
     7801 tagaaatacc agatacagaa gcagaaaact tcaaatctat aggagatata gttaagtata
     7861 tagaagctaa taaatagtcg agctttaaaa aagtggaggt atccgatgaa taaaagagta
     7921 gtaataacag gacttggatg tgttacgccg ttgggaacag gaaaagaaga gttctggagt
     7981 aatataaaat ctggagtatc tggtatagat aaaataacta attttgatgc tagcacatat
     8041 caaactcaga tagcaggaga agttaagaat tttcatccag aagaatatat aagtaaaaaa
     8101 gaattgaaga gattagataa atttgctcaa tttgcaatag tatctgctaa attagcagtt
     8161 gaagatgcaa atcttgattt agataaagtt gatagagaac gttttggtgt tataataggt
     8221 tctggaatag gtggagttga agctatagaa actcaacata aaattctttt ggaaaaagga
     8281 aataaaagag taagttcact ttttgtacct atgatgatag gaaatatggc agctggtcaa
     8341 gtttcaatat ttttaggagc aaaagggcct aacactaatg tatgtacagc ttgtgcatca
     8401 ggaactcatt caataggtga tgcttttaag gtaattcaaa gaggtgatgc agacatcatg
     8461 gtagcagggg gttctgaagc tgctgtaaca ggtcttgctt ttgctggatt ctgtaacatg
     8521 aaggctatgt caactagaaa tgatgaccct aaaactgcat caagaccatt tgataaagac
     8581 agagatggtt ttgtaatggg agaaggagca ggtattgtta tcttagaaga cttagaacat
     8641 gctttagcaa gaggcgctaa aatatatgca gaagtagttg gatatggttt aactgctgat
     8701 gcataccata tgacaactcc agctgaaaat ggagagggag cagctaggtc tatgaatatg
     8761 gctttgaaag atggaaatgt tccattagaa gaagtagatt atataaatgc tcatggaact
     8821 tcaacttatt acaatgattt atatgaaact atggctataa aaactgtatt tggagaaaag
     8881 gcatatgact tatgtgtgtc atcaacaaaa tctatgactg gtcacttatt aggagcatca
     8941 ggtgctatag aggctgttgt ttgtgctatg agtatagaag atagttttgt gcctccaact
     9001 ataaacatac aagaagttgg tgaagatttg gatttagatt atgttccaaa tcaaggtaaa
     9061 gaaaaaaata ttagatatgc actatcaaat tctttaggat ttggtgggca taatgctaca
     9121 atagtactaa aaaaatatgt ataaataaat attaaatata aaactaaaaa acaagcactt
     9181 gttttaactg tataaattta tattagctta aatttaaagt ctcctaaatt attttaggag
     9241 acttttttat taagatttaa gtctagcata tgttgtaaat cattttgata tattgtatta
     9301 ttaattatta agaggttatt tatatatgta agttttcatt ctacaatata caaaaggagg
     9361 actaaaattt tgaattttag actaccaaag aaaaggttac ccaaaaagat aatttttata
     9421 tgtataatac ttattagtat tattttgttt ataaagcatg accaaaatgg attgaaagaa
     9481 agagaaatta taaataaggg aaaatatatg tctgaaagcc ttgtggcaga taaggagtat
     9541 gatgaagcaa aaaaagtggt taagacttct ttcgaatcta ttccagaaaa aaaatacaat
     9601 aaagtatata atgaagtttg ggatatgtta aagactattt catatgttcc agatggaacg
     9661 aagatagtca ttgattcttt agaaaagatg agagaatcag atgtgaaatt atctgatgaa
     9721 tgtagattta atatagaaaa aagattagca tcagtgtata taatggataa taattattct
     9781 aaggcagtag atttaactgt aaagtctata aagttggctg agaaattggg gaataattat
     9841 gaaaaagcaa gactagatgt tgatttggcg agcatatttt taaagatagg tggctatgaa
     9901 acaggagaga aattaataaa ggattctttt aaaatagata ttgatgaagg tgaaaaaaat
     9961 ggaatggtta gattatatgc tattataaat ctagcagaaa tatatgtaga agagggcgaa
    10021 tatgataaag ctattgaaat aagtagcagg ataaaagatt ataaaaagtt tgtaaatacg
    10081 gataattaca atgattttga tataatggcc tctataatgc agtcaaatgc gtatgtgggc
    10141 aaaaataata tacaaaaggc aaagactttt ttagaaaaag ctgattatct tataaaatca
    10201 gatagaacag tttatatatt agataaagat atgtactatt atatggcaat gggaaactta
    10261 gaatataaaa gcgaaaatta taattctgca atagataatt ataaaaaaag tttagagata
    10321 tctactaaaa gaaagttaac ccaggagaaa ataaaaaatt taaataagat attaaatata
    10381 tatgaaaaac aaggaaatat agaatcttta aatgagtatc aaaagatgtt aataaatgaa
    10441 tataaagaga acaaaagtat aagagattca gagactagtt tctatataat tgataaagta
    10501 tcaaacgaaa gtaagctatt tgaaaaaact aaaaaagaaa taaaatatta taaaatatta
    10561 tttacagtaa ttttaattgc tatattaggg tctacttttt tatacaaccg attgaagtat
    10621 tttaaagcac aaaatttaca tgatggtctt acaaatgttt ataatagaaa aagttttgat
    10681 attatgtatg aaaagtacag agagaaagat gataattttg cacttgtgat gatagatatt
    10741 gataatttca agctcataaa tgataattat ggacataaat ttggtgacac tgttataaag
    10801 ggaataacaa ataccatttg tgttatgtta gaacaagatg ataaagtctt taggtacgga
    10861 ggagaagaat tttctgtatt aataagaaat aaatctggtg atgaagtagc agatataatt
    10921 gacagtataa gggttgcaat tgcaaataaa aagtggaatg atgatgtgac tgtgactata
    10981 agtgcaggtg tagctcatgt ttcagatagc aaagatgtac tagaagaagc tgataaaaat
    11041 ttatataaag caaaacaact tggtagaaat aaagtagtat ataagtaatt atatactata
    11101 taaataagag gtataaatat gaaacaatca atagctatag taactgataa aataactaaa
    11161 ctatcatctt tcttagaaga aaatatacgt ttggttttag gtaattatat tgatataaac
    11221 cactattatc tggaagtttt aaaagagaat gataaaatac aaggtgattt tgtactagta
    11281 atgaatgatg atagattaaa cagtatgaga aagcatttag tacaagatag taagattata
    11341 gtgataagga gaacacttaa agaaaatgaa atatatggat tattttcaat accaaatgga
    11401 actgaagtat tggttgtaaa tgatacaaaa gaaactacct tagaaacgat aagcttattt
    11461 tacaaaatag gagtaaaaaa tttaaagttt actccatata ttgaaggaaa aatttataaa
    11521 aatatagaca tagctataac gcctggagta aagggaaaag taccaagtta tataaataaa
    11581 gtaatcgacc ttggaaatag atatattgat atatctacct ttatagaaat aataaatggt
    11641 cttagaatag atgttaaaga aatacgtaaa aatttaatgg catattctga gagtttaata
    11701 agtttagata atggagttaa agataattat aggcaactgt ttttaaaaat agaagaacaa
    11761 gatgttatat tgaatctatc aaaagatgga ataatattta cttcagcaga tggtataata
    11821 aacacattta ataaagaagc tttaaaaatt ttaggtataa atgaaaatcc acaaggcaaa
    11881 aacatagaag atatcatttc tgaagattta aatattttac ttgaagacaa agaaataatt
    11941 gatgaagtgg tatttacaaa taaaaaatat ataaatgtaa ataaaaaaag tattttttct
    12001 atggggaata aagctgggat atattataac ttacaagaaa taacgtatat aaaaaaatta
    12061 gaacaaaatc ttactaataa attaagagaa caaggtcaga tagcaagata tacatttaaa
    12121 gatataaaaa ctaaaaataa aaatatggta aagtgtgtag aattggctaa aaaaatatct
    12181 aaatcagaac tttctgtatt aataatagga gaaagtggta ctggtaaaga attattatca
    12241 caatcaattc ataatgcttc caatagaagt aatcaaccgt ttatagcagt taattgtgca
    12301 gcagttccag atagtctatt agaaagtcaa ctgtttggat atgaaaaagg ctcttttaca
    12361 ggagctttaa gagaaggaaa aaagggtcta tttgaacttg ctaataatgg aactatattt
    12421 ctggatgaaa ttggagatat gcctccattg ctacagacaa agttactaag ggtattacaa
    12481 gaaaagcagg ttatgcctat aggttcccat aatgttataa acatagatgt tagggtcata
    12541 gcagcaacaa acaaaaactt actaaagatg attagagaag gaaaatttag agaggacctg
    12601 tattatagac taaatgtatt gccaatagat gtaccatcat tgaggaatag aaaagaagat
    12661 ataataacac ttatgaattt ctttttgaaa aacaatataa gactttcgcc agaagttata
    12721 actattttag aaaaatataa ttggccagga aatataagag agttacaaaa tgttgcttct
    12781 tatgtaagtc taatgtgtga tagtatggtt tttagagatg acttaccacc atatataagg
    12841 gtagaatgtg ctaacaatta tgaagatgga tttggttata caagaaaaga atttgaggaa
    12901 atactgagga ttctatatga aagaagagag tcatcctctg gaattggaag agggtttata
    12961 atcgaaaagc ttatacagga aggtattgag atttctgaaa gcaaagttag aaaggtgcta
    13021 tcttatttaa gtgaatgtgg atacatatca tctaaatctg gtagaggagg aagtcaaata
    13081 acctcagagg ggataaaact ttataataat ataaattaag ctaagtattt gatattgaaa
    13141 tcacatattt tgaatttaga tatttaaata ataataaact tttatattta tagagattaa
    13201 tttatattat gtcagaaaga atattgtaat ttataattaa atattaatag gtttttaaaa
    13261 taggattaga taggtaatag gttaagttag aataacccat tacctatttt tatttgtaag
    13321 aaaagtgtac atagaattag aagtaaaaaa tacaactaat aaaactattg aaaatactag
    13381 ataaataata aataaaaata attatagatt ttggcataca tattgctaat tatagagata
    13441 agacgacaaa ataattggga ggtttaatga tgaaatacaa aacaacaggg acttgtgcta
    13501 cagaaattga atttgaagta aaagaaaata aagttacaaa tgtaaacttt ataggtggat
    13561 gtgatggaaa tttaaaaggg ctaaaagtat tagttgaagg catgaatata gaagatgtaa
    13621 tacaaaagtt aaaaggaata gagtgtaaaa caaagccaac atcttgccca gaccaactgt
    13681 ctctagcatt ggaaaattat atgaatataa agtaatacat aaaacaatcg attagttagg
    13741 agtttaatta tgaaaccaat aatagggatt ttaggaaatt taataataat ggaaaatggt
    13801 atgtttccag gtcttgaaag gtcatatgta aataatgatt atataaatgc agtgctaaaa
    13861 ggaggaggta gtccagtaat aattcctgta aatacagata aagaagttat aaaaaaacag
    13921 attgaaatgg ttgatggagt cttaatatct gggggatggg atatcaatcc tcaactgtat
    13981 ggagaagaaa caagagaaga aacgacattt atatatccag aagtggatga atttgattta
    14041 attgcaataa gtatagcatt agaacttaaa aagcctatat taggtgtatg taggggacta
    14101 caaattttaa atgtaagttt aggaggaact ttatatcaag ataataatct tatagaagga
    14161 agttatataa aacatacaca atcttctaaa agacatgtag ctactcataa agttgatgtt
    14221 aaagaaggaa gtattttaga gggaatttta ggaaaacaat tacttacaaa tagttatcat
    14281 catcaaagtg tcaatcagct tggaaaagga ttaaaagcca tagcatactc aaaagatggt
    14341 ataatagaag ctattgagaa agaggatgaa aattttgtag taggagtaca gtggcatcca
    14401 gaaatgatgg tagattactg tgataaaatg gaaaaactat ttaaatattt tataaatatt
    14461 tgttcaaatt tataatatac cttgggggga ttgaacatga acgaaaagaa caaaatggga
    14521 ttaataagta ttatactttt aggtattaat gcagtagtag gagctggagt ttttctatta
    14581 ccaggagatg caatgaaatc atttggtgta gcaagtatat ttgtatatat atttgatatg
    14641 ttgttagttt tatctatggc attctgtttt gctgaggttg caggtaaatt caataaaaat
    14701 ggtgctgcct atgtatatac aaaagaagct tttggagatt tttgtggttt tgaagtaggt
    14761 cttatgaaat gggtaatagg atgtatttct tggggagctt taatagtagg ttttcctaca
    14821 tcattatcag cagtatgggc tccagcaggt gaaccacata tacaaaaaat tataattgta
    14881 gtcatgatag ttgggcttac aattataaac ttattaggtg tatctctatc aaaaattgta
    14941 caaaatgtta taactgtagg taaactaatt ccattaatac tatttatagg aataggtata
    15001 ttttttataa aaggagttaa ttttacttct tcaacaatgg tacctccagg agctggagca
    15061 acagagtttg gagcagcagc acttttaatg ttttattcat ttacaggatt tgaatctata
    15121 gcagttgcag cagaagatat ggagaatcca caaaaaaata taccaatagc aattataagt
    15181 gtaatagtaa ttgcatcaat aatatatata ttaaatcaag ttgtatgtgt tggtatacta
    15241 ggagattcgt tatctagtac atctacacca gtggcagatg ctgcaagaat atgctttgga
    15301 aatatgggag ctggattggt tacctttgga accttggttt ctgttggtgg aatttgtatg
    15361 tgtggtgctt ttgtaaatcc aagaagctgt gtagctttag ctgatgataa aatgttaccg
    15421 agaatatttg caagaaaaga taaaaaaggt acaccatatg tagcgataat agctactatg
    15481 ctaatcacta taccaatagc tctatctgga agttttgctg aattagcagc aataagtgca
    15541 gttgctagat tcatacaata cataccaaca tctttatccg tattagtgtt tagaaagaaa
    15601 agaccagaat tagtaggtac ttttaaaact ccttttggag cagtgattcc tctaattgct
    15661 gtatgtgttg gaacatggtt attattccaa gcatctatgc atcaattaat tatgggatta
    15721 ggagctttgg caattggagt accattatac tttataatga agtcttataa tagaaaagtc
    15781 tatgatgaaa atttaaagaa aatagtttaa ttatgttata acaatttatt gagttaaaat
    15841 tagacattgg cataaaatta aatacaaata agtatctaca aggctatatt aaaatattat
    15901 taatttttta ttttaatata gccttatatt gttatcaata aatgaaaaat attgtaacaa
    15961 tagaagaaca atagtattat gtttatagac aaaaaactta ttaggaggta aatttatgcc
    16021 aacaataaca ttaaaaaatg gtgtagacgt acttattaga gaaggtgtaa gagaagatgc
    16081 tcaaagcatt atagattttt ataatgaagt gggtggagaa actcattttc tgtcatttgg
    16141 aaaggatgaa tataaaatat ctttggagga acaagaaaat gctatagaat cagcaaaagc
    16201 atccgataat tctgttaaac tgattgcatt tatagatggt gaaatagttg ggattgcaac
    16261 gattgattct aatcaaaaag caaaaggaaa gcatgttgga gtattaggaa ttgttgtaaa
    16321 agaaaaatat tggggaattg gattgggtaa aaggcttatg ttagacctta tagaatggtg
    16381 taaatcaaat ggaataacca agaagataac tcttgtcact aatgaagaaa attataatgc
    16441 tataggatta tacaaaaaag ttggatttga agtagaaagc atactaaaga aagaatgtta
    16501 ttataatggt gtttatactg atttaatagg aatgtcactt ttattgggaa tataattagt
    16561 gtacatttta tctcgaaaaa agtatattaa gtatggtata attaaaaaag aatataactt
    16621 aatataattt tggaggacga attatgaaaa atctgtgtaa aatatcagat gattatttaa
    16681 acagcaagct taagtattta aagttattat caaaacaata tccaagtata tcaaaagcaa
    16741 gtacagaaat aataaattta gaggctatat taaatcttcc aaagggaaca gagcatttta
    16801 taacagatgt acatggtgaa tatgaaccat ttgtacatgt attaaaaaat ggttctggtg
    16861 taataaagag aaaaatagaa gaattgtttt caaatacaat aagagacagc gaaaaaaaaa
    16921 tgttagcaac tcttgtatat tatcctgaac aaaagttaga cctaataatt aagcaagagg
    16981 aaaatataga tgatttttat agaataaata tttatagact tatagaactt tgtaaatatg
    17041 cttctagcaa atatactagg tctaaagtta gaaaactgtt accagaaaat tttaaataca
    17101 taatagaaga acttttgcat gagcatgtta aaagtgaaca taaagaagag tattataaaa
    17161 gtatagttga aacaatagtt gacataggaa tagctaagga gtttataatt gctatttcta
    17221 cagttataca aaaattggtt gtagatagac ttcatgtaat aggagatatt tatgatagag
    17281 gtccaagacc agatataatt gtcgataagc ttattgaaca tcattgtgta gatattcaat
    17341 ggggaaatca tgatatattg tggatgggtg cagcctcagg tgaaaaaact tgtatagcaa
    17401 atgcactgag gatatcagct agatatgcga atttagacat tgtagaagac atatatggaa
    17461 taaatctatt gcctttagcc acttttgcta tagaaatgta taaagatgac ccttgtaaag
    17521 aatttattcc aaaggttaat gaccaaagtg taacaacaac agaaaagtct ttaatggcaa
    17581 aaatgcacaa agcaataagt ataattcaat ttaagcttga aggtgaggtt atcagacgaa
    17641 gacctgaatt tgaaatggaa catagactct tgcttaatat gataaactat gatgaaggta
    17701 cgattaattt aaaaggtaaa acatataaat taaaagatac ttatttacct acaatagaca
    17761 aaaaagaccc atataaatta actatggaag aaagaaatgt tattgataaa ttagtatctt
    17821 catttagagg tagtgaaaaa ttacaaaaac atgtttcatt tttgttttct aaaggaagta
    17881 tatatttaaa ggctaactca aatttactga ttcatggatg tgtaccgtta aatgaagatg
    17941 gaagctttat gtctatgaat ataatgggaa aagagtataa aggtaaggct cttatggata
    18001 agatggagtc tctagcaaga gaaggatttt tctttaaaga taaggcagaa gaaaaactgt
    18061 atggcatgga tattatgtgg tatttatgga ctgggaaatg ttcatcatta tttggtaaag
    18121 atgatatgac tacatttgaa agatacttta tagctgaaaa agagactcat aaagaaaata
    18181 agaacccata ttttaaactt agagaaaatg agatggcttg taaaagatta tttgaagaat
    18241 ttgatttaga acttgatgaa tctcatataa taaatggtca tgttcctgtg gagagtaaaa
    18301 atggagagag cccaataaaa gctaatggta aaattctcgt tatagatggt ggtttttcta
    18361 gggcttacca aaaaactact ggtatagctg gatacacttt gatatacaat tctagaactt
    18421 tgcaattagt atcgcatgaa ccatttaatt ctgctgaaga agccattgca aatgaaagtg
    18481 atatcttatc gacaactgtt gtagtagagc ataaggcaaa gagaaaaatg gtgagagata
    18541 ctgatgaagg agtaaagata caagaggaaa tagaagattt aaaactactt ttaatggctt
    18601 ataagaaagg gctaataaaa gaaatgtaga tgttatagag gcacttcatg attgaagtgt
    18661 ctttttgtta atacttatta aatatattat aaagattaat aatagtagtt tatattatac
    18721 ttactaagtg aatccaagat aaatgggttc acttttttta tttataaaca ttattaaaaa
    18781 aatatagatg ttttggttat attccttgtc ctttataaat agaattatat aaacaaagga
    18841 ggaggacaag agatgaataa actttctgat gaaataataa actctctttc tacaactata
    18901 agagaaaagg tagaaaaagt ctcaaacaat aatctgaaca tagaagaaat taggttacgc
    18961 tcacaaaaac cattgatact aaatgcaaac tctaaagatt atttttacaa tcaaaaaact
    19021 atgacacttg atttaaatca acaaaactca tatgtagtaa caagagaaga tgtagagcaa
    19081 acttttcaaa taatatgtaa atattcaata cattcattta tggatgacat aaaaaaagga
    19141 ttcataactt tgagaggagg tcatagagta gggttagttg gaaaagcaat agtagaagat
    19201 ggacaagtca aaaatataaa gcatatatca tctttaaata taagggtatc aagggaaatt
    19261 attggatgct cggataagat tttaagtcat atcataaagg gaaaaaatca gattaataat
    19321 actttgataa tatcaccacc tcaatgtgga aaaacaactt taataagaga tatagtaaga
    19381 aatttaagta atggtaatga agattatgga tttaaaggtt taaaagtggc tttagtagat
    19441 gagcgtaatg agatagcagg agcttatcta ggtgttccac aaatggatgt gggaataagg
    19501 actgatataa tagaaacttg cccaaaagac ttgggtatta caatgctttt aaggtctatg
    19561 tcaccaaatg ttattgtgac agatgaaatt ggaagtgaaa aagaaataaa ggcattatac
    19621 acagcattaa atggaggtat aggattaata actactgtgc atggagattc aatagaggat
    19681 atacaaaata gaaaagaatt aaatagactt ctggataaag agttatttaa aaaggttata
    19741 attctttctg caaaaagagg agcaggaaca attgaaaaaa tttatgattt agaagaaaag
    19801 agatggtatt ttgcaaatta aaataattat tatagctttc ttgataggaa gtagttattt
    19861 gataggtgaa caaatataca aaacttatac tcgtagacat aaacaattaa acgatttaat
    19921 aagagtttta gaaattttga ggatggattt atcttttgga ctttatacat tagaagaaat
    19981 ctttaatagg ataggtggaa ataaagagtt ttgtttttgg aaatttttct atcaaatatc
    20041 agaaggactt cacaatgaac aaagtaaaac tttggaaata ataatatcag aaaatattga
    20101 tgtcttaagt aaagaaactt atctaggcaa taaagaaatt gaggaactga aaaatctaat
    20161 acttacttta gggaaaagcg atatagagtc acaacaaaga atgatagatt tatctataga
    20221 aaatttgaaa aaacaaacct atgagacaaa ggaagatata aacaaaaaag gcgtattgta
    20281 taaaaaatta gtgacattca taggtatagg tatatgtatt atattgattt aatatgggga
    20341 ggtttattat ggaaatatca ttgatattaa aggttgcagg tgtcggtatt ctaatatcag
    20401 tattgaatat gatattggaa aaaacagata gaaaagattg ggcaggtctt actacattag
    20461 caggtgtaat aatcgtattg ggtatggtaa taacagaaat aagtgacttg tttaatactg
    20521 taagaacaat gtttcaactt tactaaagag gagggagtaa tttggaaata atgcaattaa
    20581 taggaattgc aatcatttca actacactct gtttagtaat taaaaaagat agaccagaga
    20641 tagcaaattt catagctata ataactggag taataatact attgtcagtt atgtttaagc
    20701 taaattttat agtagatagt atacaagatt tggctaacaa ggctaacatc ccgactatgt
    20761 atatatcatt aataataaaa ttaattggga tagcatactt aatggaattt gcgatacaac
    20821 tttgtaaaga ttgtggggaa ggaaacattg cttcaaaact agaatttgga ggtaaaataa
    20881 tagttatgtc aatgtctttc cctatacttt tgtccattgt agaaatggtg gtgaatataa
    20941 ttccatagat gtgtatcttg gaatgaatat aagtatatta gagagctttt gttgtattga
    21001 ggtttataga gtgaagagag gcatggttgc ttatgaaaaa aagattttta ccaatgatta
    21061 taggtttttt atttactttc ttttttgtaa atatctttgc tattatgata tttgctaatg
    21121 aagtaccaag tagtgaagat aaagaatatg gtgaaacaaa aaatagcata gataagtata
    21181 tagatggtca attagacaag ctagatataa atgagattca agactatata aataaagaaa
    21241 tagttattaa tgatgttaat ttaaaatcat ttgtaaaaga tttaattagt ggagaaaaaa
    21301 atatcttaga tttattcaat aaagatggat tgaagatatt gatgtttgat gaatttaaag
    21361 caagtttaaa ggtagtagca gtaattttgg tactggctct gttatcatct attttaaaga
    21421 gtctggaaaa ctctttttca tcaggtgcag taagtcaaat agcaacatat ataatattta
    21481 tcacaatggt atcacttaca cttattggtt ttaaagatgt attacagatt tgctatgatg
    21541 caatagacca tactgtaggt cttatgcaag ttataatgcc aatattaata acatttttac
    21601 ttttaattgg attcccaata acgtcgacta ctttgaatcc tatcttcata ggaggagtaa
    21661 catttataaa tgtatttttt aaaaattttc tatttgtatc tattactgtt gcatttggaa
    21721 ttttaataat aaacaattta tcaaaaaata taaggctaaa gagatttttc tcctttgtaa
    21781 aacaaataaa ttatgtttct attggagcta tgtttactgt ttacttaggt cttgtatcca
    21841 tacaaggatt gtatgtaacc agttttgata aattcagtgt aaagactgct aaatttgcta
    21901 taggtaattt tattcctgtt gtaggtggat ttgtatctga ctctgtggat attttattat
    21961 cttcatctca gcttataaaa aatatatttg gaggaatagg gcttatctta cttgttggaa
    22021 tttgcttact tccagtaata aaaattttat cagtaatagt agtgtacaaa ttagcagcaa
    22081 taattgtaga gcctgttgga gaggatggaa tatctaattt tttaaatgaa gtggcaaatt
    22141 taatgataat aatgctagca tcagtaattg ctataacagt aatgttcttt gtaactgtag
    22201 ctatcttgac ctcgataagt gtcgtaagtc aagggtaatt tatatatcag tgaaaaataa
    22261 ttacatttaa tctaatgttt ttagagagga ggatgctatg ttagagggca taaaggcatg
    22321 gatagttagt gttttgatag gagcatttat agtaaatata gtagatatga ttttaccaag
    22381 ttctaagata aaaccttatg tcaacctagt tttaaacttt atgtttgtat ttattgtaat
    22441 aactccagta gtaggatttt tttcaaaaga tatgagttta gaagatagaa tattaaaatc
    22501 tatgggaaac tacaacaagc agtatgtaga tagtacaaat gctttggcaa aagagactgg
    22561 aaataatagt ctatccaagg gatatgagga tgggttaaaa gaagtactta aattaaaact
    22621 tgatgaatat ggttatgatt tagaagatat agagcttaat ggggcaaata taaataatat
    22681 aaaaataaaa gaaaagaata atagtgcaaa aagtaataac agttccaatg aagaaaataa
    22741 aaataatagt actaaaagag aggaagaaaa cataaactct aatgacaaag aaaattctaa
    22801 acaagtcttc aaaaaaggta cagagtatgg gttgaaccta aatgaagaaa aattaaaaaa
    22861 cgacttaata aaggtgctgg atgtttctat agaagatata caaattgata aataggagtt
    22921 gggagaatgt tgaaaaactt aaatgacaaa gataagagaa aggtatattc actaataact
    22981 atagcaggaa tatgtgtggt ttcattagta ttattatctt gttttcctag tagcaaaagc
    23041 gataaacaag ttggaaaaac agagactaat aaaaatgctg aaaagcaagt cactaaagaa
    23101 caagaaaaag atgatttaga gtcaaaatta acagcaatac tatctaaaat tgatggtgca
    23161 ggagacgttg atgtaatggt tacatttgaa tcaagtgaag aaatacaacc tgcttttaat
    23221 tcaaataata caacagaaac aacagaggaa aaagatgcac aaggtggaga aagaacagtt
    23281 acgacttcta gtgaaaataa aacaatgata acttcaaatt caagtgaccc tgtagttata
    23341 aaaactacag agccaaagat aaagggagtg atagtggtag caagtggagc aagtgaccca
    23401 agtgttaaag agacacttta tagtgcagta caaacatcat tacaagtagc tggtcatcaa
    23461 gtagaaatat attctaaata aatacatata accgggggga taaatgaatg aagtttaatt
    23521 ataagggaag aggatttgta ataataactt taactgcaat gttagtggta gtaggaacag
    23581 tgaattatca actaagtaag aaatctttat tagaaacatc aaaagagttt aaggcatatg
    23641 aagaagcaca gttgcaaaaa aatactgatg atagttatag tagtaagaca gaggattcta
    23701 gtaatgtaga taaacaaggt ggtaaggaaa gtgctgatat tgatatagtc gatagcaaag
    23761 ctagtaaagt aaaagaaaag gcaactgaga caagtaagga aataaaagct caattatcat
    23821 cagagaaaaa tatgaaaaag gcatcttata ttttagatat gaaaatgaat agagaaaaac
    23881 aaagaaatga gttagttcaa gatttaaatg aaatgataaa taatccatct acaacagaag
    23941 aatctagaaa agaagcttct aatatgaaat taaatatagt taaaattcaa gaaaaagaac
    24001 ttcaaataga aaaccttttg agtacaaaag gatatgaaga agcgttagtt tatataagcg
    24061 ataacaaagt taatgtagtt gtaaatgaag ctaaattaga aaagaaagat gcagcaaaaa
    24121 tatttgattt agttgcagaa caagccaatg tgaagtatga aaatattaaa cttacaaata
    24181 ataatagtaa taagtaaagt ataaacctca gaattaactg aggttatttt cttgcaaagt
    24241 agacttgtat ttggtattat tatacttata aaaatagttc cttaaaatca ggaggtaagt
    24301 tttaatatgg aagataataa atttggacaa gtaaaaatat ctaatgatgt aatagctaca
    24361 atagctggtt tagcagcatt agaagttgaa ggcatagaaa ctacagcaac attaacagat
    24421 aagttgttaa aaaataatgg agtaaaaata caaatcgaag aagaggatgt taatttagat
    24481 gtaatggtta cgataaagta tggaatgtca ataccagata cagcttttaa agttcaagaa
    24541 aatgtaaaaa atactgttga gacaatgaca ggactgaagg tttctcaagt aaatatacac
    24601 attcaaggaa tcagctttaa gaaggataaa gttgataaag aagaagcaaa agcaactaag
    24661 aaaaattaat attagccctc tgaaaagtca gagggttttt tgctggtaat tgattttata
    24721 tggataatta aacttaaagg agaattttga ttgatgaaaa aagatagggc acaaaagagt
    24781 acaacaagag aatatataat gaaacttata taccaaataa atattaataa agaagatttt
    24841 gaaactttag aagataaagt agataatttt ttaaaagata attcagaaca tattataaat
    24901 agatataaag aacttgcact tcaatactca aaaaatacaa atttaaagtt agaagatact
    24961 gaaattgaag atgttataga caaaaaatat ataaatacag tatgcaaagc attgaaagaa
    25021 aatcatgaca aaatagatga gttaataaat aagcatgcta aaaactggac agtagataga
    25081 atgccaaagg tagatgtttc tatacttaga ttgtctgtgt gtgaaatact ttatttagac
    25141 actcctaata aagtatcaat aaatgaagca gtagaacttg caaaaatata ttgtgatgat
    25201 aagtctccta aatttataaa tggtatattg ggaagtgttg ttgatgaaat tgggaaataa
    25261 tataataatt ggaattgata ctagctgcta tactacttct atagcagcta tctctttaga
    25321 taagaaagtt atattcaatg aaaaaataat gttagaagta agggataatt caaaaggttt
    25381 aaggcaaagt gaagctgtat ttcaacatat caataattta gggattctta gtgataggat
    25441 aaaatctttt aaagataagt ttaatgtaga aggagtttgc tcatcaaaaa aaccgagacc
    25501 tgttgaaaac tcatatatgc ctgtattcaa tgtaggtcat aattttggaa aattattgtc
    25561 tagtatatat ggttgtagat tttatgagac tactcatcaa gagaatcata ttgaagcaag
    25621 tttattaaat agtaaattaa aaaataataa taaatttatt tctgttcata tgtctggtgg
    25681 tactacagag atacttttaa caagcaaaca agatagtcat cacaatgtat gtgatactaa
    25741 tttaggtaaa attgccaaaa taagtattaa aaaagatgat aaaagtaagt tatacaataa
    25801 ttttggatat aatatagata taattggagg aagtaaagat attagctttg gtcaacttat
    25861 agatagagta ggaattaagc ttggatataa atttccttca ggaaagtatt tggatgaaaa
    25921 tgctttaaat tgtaatctta aaatagaaag tggtctgaaa acttctgtaa gagatgggta
    25981 tatgaattta tcaggattag aaaatcaggt aaataagatt ataaatgata atggagataa
    26041 tactaatcaa aaagaatata tatctaaatt agtattagat tcagtagtta gaaatatgtt
    26101 taaatctcta gtgtatttat gtgaaactta taatgtaaat gaagtagtat ttgctggtgg
    26161 agtttctgca agtaagtata ttcttagaga attaagcatg aagttaagaa aaaagcatat
    26221 agaagcttat tttacagaac cacaatattc aactgataat gctgtcgggt gtgctataat
    26281 aggattgaat aactttttag gagaaagagt atgaaactaa gagctttaga tataagtgaa
    26341 gcaaactcat atattaaaag aattttaata aacgacccaa tactttctaa tcttaaagtt
    26401 aaaggagaaa tttctaattt taaggttcat agtagtggga atgtatattt atcacttaaa
    26461 gatgaaacat caaagttaaa ctgtgtaatt tttaaaagta attttaatcg caatttaaaa
    26521 ttagataatg gtgtaaaaat tatagctaat ggatatatat cagtctatga aagagatggt
    26581 gcgtatcaat tatacattaa tgaaattgaa atagagggaa taggaaattt gcacatagag
    26641 tttaatagat taaaggaaaa acttaataaa gaaggattat ttgaccctaa atacaagata
    26701 cctataccta aaatgccaaa ctcaattggc gttataacat ctccaacagg tgctgttata
    26761 agagatataa taaatgtaat aaaaagaaga tatccgaaag ttaatataaa gctttatcca
    26821 gtaacggttc aaggagataa atcagctgaa gaaatctgtg aagcaattag atttttcaat
    26881 catatgaaga atgttgatac acttatagtt ggtagaggtg ggggttcaat agaagaactt
    26941 tggtctttta atgatgaaat ggtagcaaga gaggttttta attctcaaat tccgataata
    27001 tcagcagttg ggcatgaaac ggattttact atttgtgact ttgtgtctga tatgagagcc
    27061 ccaacgccat cagcagcagc tgagattgct actccatcat tggatgatat aaactataaa
    27121 cttggaaaca taaaaagtag gatgagtaag tctcttacaa atcaaataga gctagaccaa
    27181 tataggttag aaacagtatt taataagata aataactatt tagattcgta cacaataaaa
    27241 gataaagtta tacaattaga caaaatatat gataaaataa tttttggaat agaaaataat
    27301 ttaaaattag aagatgaaaa attagtaaaa attggagcat tactccataa tttaagtcca
    27361 ttagctacta tggatagagg ttatagtata actcaaaaaa atggaaaagt aataaatagt
    27421 ataaaaggat taaaaataaa ggatagtata gatatagtat taaaagatgg aaatttagaa
    27481 tgtatgatag ataaaattga aaataaagag ggatagtatg aatttaacct acgaagaagc
    27541 ttataaaaga ttagaaagta ttttaagtga attagaatct aaaaatgcta gcttggatga
    27601 atctttaagc ctatatgaag aaggtataag cctttataaa cattgtaata agcttttaga
    27661 tgatgctaaa ttgaaaataa gtaaatttaa tcaattggga atagaagaag actttaaaat
    27721 agaggaggaa taaaattgga atttaaacaa tgtctaaaag aaaaagcaag ttttgtagaa
    27781 aaagtactta aagaatatat gcctaaagaa gaagggtatc aaaaaactgt tattgaagct
    27841 atgaactata gcttaagtgc tggaggtaaa agattaagac caatacttac attggaggct
    27901 tgtaaaatag ttggaggtaa tgaagatgaa gctattcctt ttgctattgc aattgaaatg
    27961 atacatacat attcactaat acatgatgat ttgccagctc ttgataatga tgatttaaga
    28021 agaggaagac caacaaatca taaagtatat ggagaagcta tggggatttt agcaggagat
    28081 gctttgttaa actatgcttt tgaagtaatg cttgcaggct caatcaataa agaaaatcca
    28141 gaaaagtatc taaaagctat aaatgaaatt gccaaaggtg ctggtatata tggaatgata
    28201 ggtggtcaag tagtagatgt agaaagcgaa aataaacaaa ttgaaaaaga aaaattagat
    28261 tatatacata tgaataaaac tgctgcaatg atggtaggct gtatgagagc aggagctact
    28321 ataggtggag caaattctga acaaatggaa gaaataacta aatatgcaaa aaatatcgga
    28381 ttatcattcc agatagtaga tgatatacta gacatagtag gagatgaagc taagttaggt
    28441 aaaaaggttg gaagtgatat agaaaatcat aagtcaacat acccatctct tttaggttta
    28501 gataaatcta aagaaattgc acataatttg attgatgaag caaagaaaag tatagaaaaa
    28561 ttatctgatg atgtagattt tttaaaggga ttggctgaat atataataga tagagaatac
    28621 taaaatatac agctaaaatc gaggagggaa aaatggattt tttttcggag atttttaaca
    28681 atggggcttt gggaataagt ctgattgctt gtttcttagc acaatttata aaaatattta
    28741 ctggtaagga aaaaagaatt gagttatcaa gaatacttat ttcaggaggt atgcctagtt
    28801 cccatagttc ttttgttaca agtttagcta cagtggtagg tattgaaaaa gggtttaatt
    28861 ctactgattt tgctataatt accgtgcttg ccttaattat aatgtatgat gcagcaggtg
    28921 ttagacgggc tgttggtaaa caagctacta tattaaatca aatggtggca gatatacagc
    28981 atggaaagca tatagagcag aagaaattaa aagaattaat aggtcataca ccactagaag
    29041 tttggtttgg agctttactt ggaatagtaa cagctttaat attgatgtaa attgaaaatt
    29101 tacagaataa aggtgaggat aatatgtata aatatttaga taaagtaaat tctccaaagg
    29161 atataaaaaa tatgagtata gaagaaatgg atttacttgc aaaggatata agaaagttct
    29221 tagtaaaatc tgtttcaaag acaggaggac acttagcttc taatctagga gtggttgagt
    29281 taacattagc acttcataaa gtatttgata gccctaaaga taagattgta tgggatgtag
    29341 ggcatcaatc ttatgttcat aaaatagtta ctggaagaaa agattgtttt gtatctttga
    29401 gacagtttaa tggactaagt ggttttccaa aggagaatga aagtcctcat gatatttttg
    29461 atacaggaca tagcagtaca tctatttcta tagctacagg tattgcctgt gctagggata
    29521 taaaaaaaga aaactatagt gttatatctg tgataggaga tggttctata acaggaggta
    29581 tggcacttga agctttaaat caattaggat atatagatac caatatgata gtaatcctta
    29641 atgataatga gatgtccata gataaaaatg ttggaggaat gtctaaatat ctgtcaagta
    29701 ttataagaaa ctcaacagtt gagaaaatga cagatgaagt tgataaaatt ttaaatgtta
    29761 cacaaacagg tgaaatttta tctaagactg cacatagatt taaagataaa ctaatgtata
    29821 gcttttctcc tcaggactgt tcattttttg attcattagg tataagatat tatggaccta
    29881 tagatggcca taatactaaa gaactaatag atatattgag aaaagctaaa cacaaaaaag
    29941 ggcctgttct tttacatgta attacgaaaa aaggaaaagg gtacagattt gcagaagaac
    30001 aacctgataa atatcatgga gtatcaaagt ttgatataaa gacaggtgtt acatctgcaa
    30061 aagttaagtc tatgtcaatt agtgttggag aaaaacttgt tgatatggct aatagtaatg
    30121 aaaatatagt agcaataaca gctgcaatgc catctggtac agggttaaac ctgtttgaaa
    30181 gtgcatatcc aaaaagatat tatgatgttg gtatagctga acaacatgca acaggatttg
    30241 cagcaggtct tgcaaaaaat ggtatgaaac cttactttgc tgtgtactca tcatttttgc
    30301 aaagggcata tgaccaagtt attcatgatg tatgcataac aaaaaagcca gttacttttc
    30361 ttatagatag agctggatta gttggaaatg atggagaaac tcatcatggt atgtttgatt
    30421 taagttactt aaattctatt ccaaatattg tagtaatggc tccaaaagac acaagagaga
    30481 tggagcttat gatggatttg tcattaaaat tagattgtcc actagcaatt agatacccaa
    30541 gaggaagtag ttattaccta gataagggag agtatggaga gattgtactg ggtaaatatg
    30601 aggtattaga tgacggtcaa gatacagtca tactttgtat tggaagtatg gtaaaacatg
    30661 ctttagaggc taaagaaata ttatcaagag aaggaataaa tccgacaata gttaatgcta
    30721 gattcttaaa gccaattgat gaaggtatgt taaaagcatt attaaaaaat cataaaaatg
    30781 tagttactat agaggataat attgtaactg gaggatttgg aagtagaata aataagttta
    30841 ttatagataa tgaatataac gtaaatatac ttaatatagc aataccagaa gaatttgtaa
    30901 aacatggcaa tatagatgaa ttatatgact ttgtaggatt atcgccaaaa agtattgctg
    30961 ataagataag gaaattagtt attgagtaaa aatctaatgt agatattgta aattaaaatt
    31021 tatataaaat atagaattta tcaattagaa taaatatcaa gtgtgttact tcaagctact
    31081 ttagttatca ttaaagtagc ttttaacaga aaggtaattg tatgaaaaaa agaatagatt
    31141 tattattagt agaacaaggg tactttgaaa gtagagaaag agccaaaaaa gcaataatgg
    31201 ctggattggt ttttgttgat aatcaaaggt gtgataaggc tggtacagaa gtaaaagaag
    31261 attgtagtat agaagtaaaa ggaaatccaa taccatatgt cagtagaggt ggtttaaagc
    31321 ttgaaaaagc aatgaaaaac tttgatttga ctattgatgg aaaggtatgt atggatatag
    31381 gggcatccac aggtggtttt acagattgta tgctcaaaaa tggagcaata aaagtatttt
    31441 ctatagatgt tggttatggt caacttgcat ggaaattgag acaagatgat agggttgtgt
    31501 gtatggaaag aaccaatata agaaatgtga caatagaaga tacaaagcag tttgctgatt
    31561 ttgcgtcaat tgatgtgtct tttatatcgt taaagttagt acttccaaag gcaaaggagt
    31621 tagtacgtca tgatggggaa gttgtcgctt taattaaacc tcaatttgaa gcaggaagag
    31681 aaaaagtagg taaaaaagga gtagttagag aaaaatctac acatatagaa gttataaaaa
    31741 tgatttctga tttctcagtc gaaaatggat ttgagatttt agggttagat ttttctccta
    31801 ttaaaggtcc tgaaggtaat atagaatatc taattcattt gagaaatgga aatgaaggat
    31861 atgaatttga tggtgagaca tacaataaca aaatagtaga agttgttgaa gcttctcata
    31921 atttagacaa ataggttaaa agggggctta gatttgatcc tcgaattata tatgaaaaat
    31981 tgtgccttag ttgaagaact aaggttgaat attgataaaa acttaaatat acttacaggt
    32041 gaaactggtt caggaaaatc tataataata gatgcacttg gactttgttt aggtgataaa
    32101 tatgataggt cttttttaag aaaaggaaca gataagggac ttgttgaggc tgtatttttt
    32161 tcagacaata gatatcttaa aaaaatatta gaagaaaatg atataagtat ggaagatgat
    32221 aatttattgg taataactag gttgatatac tcagatggta agagtactgc tagagtaaat
    32281 ggaaggactg ttaaagtgtc tcttcttaaa gaaattgcat ctacacttat agatatacat
    32341 ggtcaacatc aaaatcaagc tttgtttaat aaagatactc atttaaaatt tctagacctt
    32401 tttggagaaa atgaattgga agagtttaaa attgcttata aaaatgtgta ccataagtat
    32461 agtgaagtaa aaaaagcttt aaattgcttg acagaaaata aagatgagat gcaaattcaa
    32521 cgagaaatag atttattaag atttcaaata aatgaaattg aagctgcaaa tctaaataag
    32581 aatgaatatg aagatttatt aaaacaaaga gaagtttata gaaatagtga aaagatatat
    32641 aataatctaa attcgagtta tagcaagtta cataatggtg agtataatgt tattgaccta
    32701 ataggtcttg catctaagga acttaatgac atatctaaat atgacagtgt tttaagtgaa
    32761 tatagtgata ctgtagaaag aatcatgtat gagttacaag acatatctgg tgagataaga
    32821 aattacaaag ataatataga ctttgaacct tatgagctag agcagattga gcttagaatc
    32881 gacgagatta ataacttaag aagaaaatat ggagacagta tagatgatat atttgagtat
    32941 tatggaaaaa ttaaagatag attagatgaa atattaaaca gagatgaaag agttgaacag
    33001 ttaagaagca aactgatgaa tatagaagaa gatttaaaaa taaaagccag taaattgact
    33061 aaagcaagaa tagaagtagc aactacttta gaaaaaatac ttcttgatga attaaaaagc
    33121 ttaaatatga aaaatgtaat gttcaaggtt aattttgaaa aaggtccatt tactttagat
    33181 ggagtagatg atattgaatt tatgatatct tttaacttag gagaagatat taagcctata
    33241 tataaagttg catcaggtgg tgagatgtcc agatttatgt tagcgtttaa aaccatattg
    33301 gctgatatag atgatattga tacattagtg tttgacgaga tagatactgg aataagtggg
    33361 atagcagcac agatagttgg tgagaaattg agtgatatag caaagaaaaa acagataata
    33421 tgtataactc atttacctca aattgctgca aatgcagaca ctcattattg tatagagaaa
    33481 gatacttcaa ataataggac gtttaccaat gttagtaagt taaatgaatc tcaacgaaaa
    33541 aatgaaattg ccagacttat agcaggtaac aatataacag aaaaaactat tgaacatgca
    33601 agtgaaatta ttgagttggc taaaaaatgt tagtgaaaaa caagcatttt aataggtgta
    33661 aagaattttt atagaaatgc tttacatcta ttttttatga tgaaattatt ttaaaatatc
    33721 tagtgcctat attgaagaaa agtaggtttg aaaatggaat gaactttgta taaatttgta
    33781 actttgaaat ttctaaatgc ttaagtataa tattatttat taaggtagta gtgaggggag
    33841 gcataaaatg aataaaaaag atactatgca cttaattttt ataattgtaa tttttatttt
    33901 attatggtat attgtggtag cagtaagtga gattaataaa aatattgaaa gttgtagtaa
    33961 caaattagat tatttgtcac aagagcttat gagtactaag tcggatatta gtaatactat
    34021 aaatgaagaa atgtcaaaaa gctatataac aaaaagtatt gacttaaaag tgaaaaaaat
    34081 cgagaaaaaa gaatgtatta ttgatataga tgttcaatta agtaaacttg gaaaaaatgg
    34141 aaaagtattt tttatgtata aagaagatag tgataaatgg aatgaaactg agatgacaaa
    34201 acatggagaa ttatcatatg catgtgaaat taagataaat acaggaagtg aatatgacta
    34261 taaagtagtt acaagtggag ctatatctga aagttctgat gtgcaaaaat tgactaaatc
    34321 tgattatatg cctgaacaac ccatatttaa ttctgggata agagataata gagaatactt
    34381 tattgaaata gtagaaaata gtaatctttt caatcatgag agtgaaaagt caaaaaataa
    34441 agtttatgat aatattagat tagaaaaagt agatattatt gtaaatgaag gaaaaaaaga
    34501 cactatatac aaagccaaat tagctgaagg cttagatgat ggcaaaacta ataattcaaa
    34561 tagaaatcaa gtgaattatg aagtaagttt tcctaaaagg gatattgatg acataattta
    34621 tattaaagct aaacttacat acaataatgg agtagaatat acaaaggata taactgaaga
    34681 tataactatg ggattacaag atttagaaaa ataaatacat atttaaataa atattcaaat
    34741 agatttgttc aataaaatag agtataaata aaactgtagt agttttactt atactctatt
    34801 aaaaaattat aattttaatt ccatatatct agccccttca attggattat aagtataagg
    34861 ttctatatca taaaaaccaa acttttcgta taatccttgt gcacttttca tgcttggaag
    34921 agtatctaat ctcatgtgtg tatatccaaa ttttttagct tcttcaatga tttcttccaa
    34981 tagtattttt cctattttta atcctctaaa ttgatttctt acatataatc tttttaattc
    35041 acatatttta ccttcaagtt tttttaaagc aacacaacct gccaaatttt catcaacaaa
    35101 tgccaatatc aatgagcctg aaggtttttt atattttcct ggaagtgttt ttaattcatt
    35161 attaaagtct tgaaaacata agtctatatt tagagaattt gaatattcag taaatagaag
    35221 ttttacgttt tcaaggtttt ctaaatcttt ttcttcaaca aattgtaaag ttatattttg
    35281 agccatttta tccgccatta tatacacatg tttatataat gacttcacct ccttgatata
    35341 tgtatatttt tcttaatatc aattagattt taataccatt actttttcac caccatagtc
    35401 aagctctcca ttaaactcaa atccaaaatc tttataaagc ttaatagcca ttttattgtc
    35461 ttcgaatata ctaagatata gttcatcaca gttatattca tttttaagat gatttacaag
    35521 aaaatctaat gatgcttttc catatccttt accttgatat tttgaatcaa tcataaatct
    35581 atcaagccaa actctatcat cttctaaacg accatacata gcaaatccta ttaaagaatt
    35641 ttcatcatat attccaacag gtacccaagc tgttataaat ttagattcag caatagaaag
    35701 tgcattgctt tctatataat ctaactgact tttatctaca gacaaactaa ctaaatcatg
    35761 ccaattattt atgtctgcaa ttcttacatt taatttcaag atattaccgc ccttcaaact
    35821 ttttgttaca atctaatact atcatattat gtattaaaaa tcacttatta agattttaaa
    35881 taaacttttt aaatgaataa attctcagga aaaaggaatt ataaatacat aataatacag
    35941 cataggagta gatggatata tgaacaaaat aaataaaaat ttcaaaaata tttggataat
    36001 attaggagct gtatcgattt ttaccatggt tataattttt aataaaaatt gtaatgttaa
    36061 taaagagact caaatagaag cttttacaat taagaatcat gataaaaaat ttgtatatcc
    36121 tatgggaaat ataattggag taaaagcaaa tacagatggt gtattggtct taggatatga
    36181 ggaagaggat gtagactata ttggaggcat acaaatcggt gataatatag taaaaataga
    36241 taataaaagg ataaaaaata gtcaagatgt atcagagatt ttgaataaga ttaaaaaaag
    36301 taaagttgaa gtgacttttg agagaaaaaa taagtataaa acagaagtaa tagagactaa
    36361 aaaagaaaat ggcaagtata aacttggatt atgggtaaga gataaaatat ctggaattgg
    36421 aactatgact ttttacgacc ctagtatgga aaagttcaaa gcaattggtc atgccataaa
    36481 agattcggat actaatgaac tattaaagat aaagcaagga tatatatata agccagaaca
    36541 attaaaaata gtaaaagcta gtaatgaaaa agtaggtaaa ataaaaggag attttaatga
    36601 cagcaattta atgggtaatt tctcaaataa ttcagagtta ggtataagtg gtaatattac
    36661 agaaaaccat aataaagagt ttaatgtagc aaataaagaa aagcaattaa tagaagttgg
    36721 aagaccgcaa gatgtaaaaa taggaggtgc agtaattctt tttgaggata aaaacaaaaa
    36781 tataacaagc tatgatataa aaatagaaag tatagtatat gataaaggaa attatagaga
    36841 tatggtaata aaagtagtag atgacaagtt attggaatac acaggaggta tcgtacaggg
    36901 gatgagtgga gctccaataa tacaaaataa taaaattatt ggtgcaataa ctcatgtttt
    36961 tagagataat ccgaaaaaag gatatggtat ttttatagat gaaatgataa aattgtaggt
    37021 gaggcattaa aaaattttat tattttatca attatctagg aggaatataa ttttggagtg
    37081 tcgaatatgc tttagagtag ataattagga agcaattgtg taaaaagttt agttttctgt
    37141 aataagaaga tgttttttaa tggggggatt tttagtggaa aaaatcaaaa tagttttagc
    37201 agatgacaat aaggattttt gtcaggtatt aaaagagtat ttgtctaatg aagatgatat
    37261 cgatatatta ggcatagcta aggatggaat tgaagcatta gacttagtaa aaaagacaca
    37321 accggactta ttaatactag atgtaataat gccacatcta gatggattag gtgtaattga
    37381 aaaattgaat actatggata tacctaaaat gccaaaaata atagtactat cagcagtagg
    37441 tcaagataag ataactcaaa gcgcaataaa tctaggagca gattactaca tagtaaagcc
    37501 atttgatttt gttgtgttta taaacagaat tagagagcta gtttctaata gagttacaca
    37561 agttgagcca aaacctagac cagttcaaga gactcaaatg actagaagtg attttgttaa
    37621 gaatgtagga aatatagaga cagaaatcac aaatataata catgaaatag gagtaccagc
    37681 tcatataaaa ggatatttat atttaagaga agcaataaaa atggttattg ataatgttga
    37741 gcttttaggt gcagtaacaa aagagttata tccaagtata gctaaaaaat tcaatacaac
    37801 accaagtaga gttgaaagag caataagaca tgcaatagag gttgcatgga gtagaggaaa
    37861 agttgacaca ataaatcaat tatttggata tacggtacac aatactaaag gaaaaccaac
    37921 taattcagaa tttatagcaa tgattgctga taaattaaga ctagaacata gtatggttaa
    37981 ataaacaaga cataaaaagt aaggcttttt aattaaggca ttggctataa atgcgtatta
    38041 caagcagcga aacggtataa ccactagggt tataccgttt cgctatttta ataaatataa
    38101 aaattttatt tattatttgc ttactatatc aatataataa ttttattata ctatggatat
    38161 agtatgtgtc tttacaagtt gtaaactgac agtggtttat tttttaatat aaatattgac
    38221 tttgatgcag gtaaactttg tatttttaag cgtattatgg aatatgttaa ataaaaaaat
    38281 gatgaaatat agtattgtaa atgccaaaga tgcaaaacaa acttaaaaca tttattttat
    38341 tgttaagtaa tgctataata taatgtgatt ttaataatga tagtggaggt ttaaatatga
    38401 gagtcgaggc ccctataaaa gtagatcgaa aaaccaaaaa acttgctaaa agagttgaaa
    38461 gtggggaaat agcagttata aatcatatag acatagatga agttgctgca aactctttag
    38521 tagaagctaa aataaaactt gtcataaatg cggctccttc tataagtggt aggtatccca
    38581 ataaaggtcc agggatatta actgaaaata atatactaat aattgataat gttggagaag
    38641 agttatttga agaattaaaa gaaggagaaa ctatagaagt tgtagatgga aagatatata
    38701 gaaaaggaaa atttttaggt gcaggagaag ttttagacaa gtatgaggtt tcacaccaaa
    38761 taaaggctgc ctatgagaat ttagctgtag aattggatag atttatagat aatactatag
    38821 attatgctaa aaaagaaaaa ggatttatac ttggagaagt agaaattcca aaagtgaaaa
    38881 ctaattatgc taataaacat gtattgatag ttgtaagagg tcaggactac aaagaagatt
    38941 taagtactat gctttcttat atagaagagg taaagccagt attggttggt gttgatggag
    39001 gagcagacgc tttaattgag tttggatata ctccagatgt gattgtaggt gatatggata
    39061 gtgtaagtga tgaagcttta aaaaaggcta gtgaaatagt agtacatgct tatacagatg
    39121 gaagagcacc aggtcttaaa agggttgagg aattaggctt agatgcagtt gtatttcctg
    39181 caccaggaac tagtgaagat atagctatgc ttatcgctta tgagtataag gctgagttaa
    39241 tagtagcagt tggtacacat tcaaatatga ttgattttct tgaaaaagga agaaaaggta
    39301 tggcaagtac ttttttagtt agactaaaaa taggttcaaa gcttatagat gctaaaggcg
    39361 ttaatttgtt atacagaagt aaattaaaaa taaagtacat atgggcatta atagcaacag
    39421 cattatttcc tgtgttggta gttgcatcat tgtcaccagg agttcaacag ttcatacaac
    39481 taatgcaatt aaagtttaga gtattattac agatgtaaat tttaggagga taaatatgca
    39541 tattaatatg aaatattata tagtcactat aggggctata tttatagctt tgggtatagg
    39601 catgttagta ggatttaatt taaataataa tcaacaatta agtgaacaac aagcaaacat
    39661 aataaatgat ttggatgata agtttgatat cttaaaagaa aaaaatgata aacttgacag
    39721 tgacttagca agtgtaaata gagattatga agaggcagtt aattttataa ataaaaatgt
    39781 agataagatt ttagctggtt cgctgaatgg taaaagtata ggaataatat ctgctaatga
    39841 aaatgacgat tatacaaaaa atatagaaga cataataaat aaatctaatg gaagcattgc
    39901 atttaatata atattaaaag aaaacataac aaatccagaa aagttaaagg aaatgtcaac
    39961 taaactaggt acagatgtaa aaaatgcaaa tgatgcagtt aattatataa ttgacactct
    40021 taaaaaggaa gatgcaagtg acattttaac atatcttcaa gaactagatg taataaagtt
    40081 taattttatt ggagatacat atctaaaata tgattccgtt gtaatagctg gtggaaatga
    40141 tgcaaaagac agtacaaaac agtttgaaaa aatagaaaaa tttgtagttt caaaacttaa
    40201 atcagagaat aaatatttag tagaagttca aaatacagga gttaaaactt cttatgtaga
    40261 actatattct aaaaacaaag ttgcaactat agacaatatt gatgaaggta taggaagtat
    40321 atctctagct attcttctac aacaaggtaa tatagttggt aattttggta gattggatac
    40381 tgcaacttca ttgttgccat ctattaaata gaaataattt taaggagaga aaaaatggat
    40441 tcttatataa gtataataat accagcttat aatgaagaaa gtagaataaa atatacatta
    40501 gaaagcatat tggacattaa gcaaataaat gaaataatag ttgtggatga tggttcaatt
    40561 gacaatactg ctaaggttgt atctgagcta gaaaatgaaa agattaaatt ttttaagtta
    40621 gataaaaata gaggtaaagg atatgcttta aattatggta tcaaattagc aatgaaaaat
    40681 gcagatatta taggttttct ggatggagat ttaggaagtt ctgctaaaga agttgaaaaa
    40741 ttaatagtac ctatattaaa tggtgaggcg gatgttacaa tagccaagtt tcctcctgca
    40801 aagagaaaag gaggtttggg atttgtaaaa ggtttagcta aaaattctgt gtttgagatg
    40861 actggtgttg agttagatgc aactttatca ggtcaaagaa tatttaaaag agaagtttta
    40921 gaaaagtttg atgaaatacc ttttggatat ggagtagaag ttggaatgac cattgatata
    40981 ttaaagtatg gatttaaaat taaagaagta ttagtaaata tgactcataa tgaaacagga
    41041 agaaatttaa aaggttttat tcatagaggg aaacagtatt atcacataaa aaaggtttta
    41101 ggacaaaaaa agaaagaatg gaggtaatat cttagtgaaa gattatatac tttataccat
    41161 tttattatta actggattat ttggaaccta tgctgtaatt ccactattta aaaatttatt
    41221 gataaatgga aatgtactta gaccaaacta taaaaaagat atgataccag taagtatggg
    41281 aattgtattt ttgcctatga ttgtaatcaa tggaataata cttgctttct ttactacaga
    41341 atttaaggat ttagcgtata tatttatgtt tatgtttggt atgatatcca tgttttttgc
    41401 agggatacta gatgatgtca ttggaaatag agatgtaagt ggtttaaagg ggcattttaa
    41461 aagtttattt aaaggaagtc ttacaacagg aggttttaaa gctttatttg gtggatttgt
    41521 agggcttata gtttctgttg ctatatcaaa agacataatt gatataatag taaatacact
    41581 aataatagct ctatcaacta atttaatgaa tcttttggat ttaagaccag gaagggcaat
    41641 aaaagcatat ttattcataa tggtaattat atttttaaca ttgacaggat ttgttcaagt
    41701 tcttccatta cttatagtac caaatgtact tgcatacttt aattatgatt taaaagcaag
    41761 ggctatgatg ggagatactg gttcaaacgt ccttggaata tctattggaa tgctcatttc
    41821 ttttgggtat ccatttaaca ttagattagg atggttaata tttgtagtta ttatgcatat
    41881 attcactgag aaatactcat taactaagat aatagaaaaa aataaactat taaattttat
    41941 agataaattg ggaaggtagt attatgataa gcaaaagagt ggggattgtt gaatcaatag
    42001 taagtcaaac tgaaacacta gatgatataa gagtaaatat aaatggagaa atacaaaggg
    42061 catataatta tcccaaaata tctggaacta taaatatagg tgacgaagtg gttttaaata
    42121 caacagcagt agaactgagc ttaggtacag gtggatacca ttttgtaata actaatttaa
    42181 ataatataga atctacttta actgaaggtg gacatataat gaaacttaga tatacgcctc
    42241 ttcaaataaa agtggattct gttgaggaac aagaaagtat ttatcatgat aaatttgcga
    42301 attttaaagg attagaaggt ctttctgttg tagtagggac tcttcatagt atgttaactc
    42361 catttgcagc ttcatttaaa agaaataatc caaataaaaa attagtgtat ataatgactg
    42421 atggagcatc tcttccaatt tatttaagta aaaatgtgga tactttaaaa gaaaaaaaat
    42481 taatagatag tacaataact ataggaaatg cgtttggtgg agattatgaa tgtataaata
    42541 tatatactgc attaattaca gcaaaagaaa tactaaaagc ggatgcagta tttgtcagta
    42601 tgggacctgg aattgctggg acaggaacaa aatatggatt tacagggatt gaacaaggct
    42661 ctattcttga tgctgtaaaa aaacttgaag gaagagcaat tgcaattcca agaattagtt
    42721 ttgctgacaa acgagaaaga catcaaggta tttcacatca tagtatgact gtatttaaag
    42781 aaattgtaaa tgtaaatgta gacataccaa taactatata tgatgatgaa aaattgaact
    42841 ttataaaaga gcaaatcaat ttaaatggtt tagacaaaaa gcataatata atttatatag
    42901 ataataataa aacgaaagag gatttagact attttaatct aaaagtaaag agtatgggaa
    42961 gaaattatga acaagatgag gcatttttca aagctgcaag cacagcagca tattacttaa
    43021 tggaggttta agatggtatt agaagaaaaa actattagta gtgatagggt ttatacaggt
    43081 aaagtaatta ctttaaaggt tgatactgtt gaaattccag ggcaaggata tcaaaaaagg
    43141 gagttagtag aagttggtgg agcagtagga atagttgcaa tcacagatga taataaagtt
    43201 gtgttagtta agcaatttag aaaacctata gaaaagccaa tctttgaaat tccagctggt
    43261 aaactagaaa aaaatgaaag tccaaaagaa tgtgctgaaa gagaattaaa agaagaaaca
    43321 gggtatagtg caaaaaatat taagctaata cataaatttt tcacttcagc aggtttttct
    43381 aatgagataa tgtttgtata tttagcaaca ggtcttactc caggagagaa taatttagat
    43441 gcagatgagt ttttagatgt ctatgaaatt gagcttgaag aagcatataa catggtatta
    43501 aaaaatgacg ttgaagatgc aaaaacatct attggattat tattagtaaa agatatgttt
    43561 aaaaattaat atttataaaa tgaataaccc tttgtcctaa agcatattat tttataaagg
    43621 acaaggggga attaaattga gaagaacata tagaaaaaat gattttagag aattaaatag
    43681 acaaataatt attataggat tgttgtttat gatgtctatt gttgttgggt cttacataaa
    43741 taaaatattg ccaggttcta gtaacaatat attaaacaat ataaatcctg ctgtagagta
    43801 ttataatcta aatataagca taaaagatac agtgatacaa aatttaaaat cagatgcaat
    43861 ctttatgggc tcaatagctc tattaagtct atttgtagta acaattccag cagtgttggt
    43921 agcttttgta ttaaaaggga tgtcaattgg ttatacaata aatagttgta tattggcatt
    43981 aaaatttaaa agtataaaaa tgattttaat aatattattt aaaaatttga ttattattcc
    44041 atgtgcaatc atattagcat taattagttt aagttatttt aaagaaatgg tttatgaatt
    44101 taaaaagaaa aatagaaaaa atatgcaatt tttaattaag aggtatatac taaatattat
    44161 aattataata gcactatcat tggggttgca attaatatta aatactgcaa gtattggtat
    44221 tattaagttc ctagctaaat aaaggagttt ataatggata ttatagaggg atatatagat
    44281 tacataaaag ataaaaaaaa attatcagag aatacagtgg catcatactt tatggatata
    44341 aaaaaatata tggaatatat aaatcaaaaa agcattaaac tagtagatat agtagaaaat
    44401 gatattataa gttacttaat aaagttggaa aaagataatg tatcaatagc tacaatagcg
    44461 aggatgatat cctctataaa atcttttcat gattatttat ttttaaatca tatatgtaca
    44521 aataatcctg caaaggatat aaaaaaacct aaaataaaaa aagaaaatat aaatatactt
    44581 acagaagaag aaattgaaag actattaaat tttccaaagt taacaacacc taaattgatt
    44641 agggacaagg ctatttttga agtattatat ggtactggta taaaagtatc tgaattagta
    44701 gagatgaata tagaggatat tgacctagat attgattata tatattgtaa ttcatgttgt
    44761 aaaaatcaaa gagtgatacc actttgtgat attacaaaac tttacttaga aaagtatcta
    44821 aaagaagcaa gacctaaaat ggctgtagaa ggtgaaaaat cattattcgt aagttcatta
    44881 ggtcaaaggt ttacacgaca aggtctttgg aaagtcataa aaaaatattc taatctagcc
    44941 aatatagata aaaatataaa tcctacaatg cttagacatt catttgctat tcatttgtta
    45001 aatgaaggtg ctaacattgc tgttgtaagt aaaattttag gaaatgttaa tttatctagt
    45061 ttgcaagtat atttaaatca tatagataaa aacgtgagaa gagaaataaa ggagaaacat
    45121 cctagaaatg atgttgatgt agaattacaa aaagcagaag ctaaatagac tatatagcaa
    45181 attttataat ttagaaggag catatttata tgagcagagt aatttggatt gtaattgata
    45241 gtgttgggat aggagcattg cctgatgctg aaaaatttgg tgatagtaag gatgtaagta
    45301 cccttggaaa tatatttaag gaatatccag atattcagat tcctaatatg agaaatttag
    45361 gtataggaaa catagatggt atagattttt ttgaaagtat aaaagaacca ataggttgtt
    45421 ttggaaagtg taaggaaatg tctcagggaa aagatactac tacaggtcat tgggaaatga
    45481 ctggtataat agttgacaaa ccatttaaga catttgaaca tggattctct aaggagataa
    45541 tagaagagtt tgagaaaaaa actggaagaa aagtagtagg aaataaacca gcttcaggaa
    45601 ctgtaataat agatgagtat ggagaacatc aaataaaaac tggagatgta atagtatata
    45661 catcagctga tagtgtattt cagattgctg ctaatgaaga agtaattccc ctagaagaac
    45721 tctataatat gtgcaaaatc gctagggaaa taatgatggg ggataatgca gtagctagag
    45781 taattgctag accattcata ggtaagaaaa aaggtgagtt tgtgaggact tcaaatagaa
    45841 gagattattc actagaccca tttgaaccaa cagttcttga taatataaaa gaatcaggac
    45901 ttgatgtttt agcagttgga aaaatagaag atatttttaa tggaaaagga attacagatg
    45961 ctatccacac taaaagcaat atggatggag tggatgaaac tttaaattat atgaaacaag
    46021 ataataaagg tctaatatat tcaaatctcg ttgattttga ttctaaatat ggtcatagga
    46081 gagacccaga aggatataag aaagctcttg aagagtttga tagtagactt cctgaaataa
    46141 tggctaatat gagagaagat gatattttaa taatcaatgc agaccatgga aatgacccaa
    46201 catataaagg tacagaccat acaagagaat atatacctgt tatgatttat ggcaataaga
    46261 ttaaaaaagg ttttaattta ggagtaaaag atacttttgc tgatattgga gcaactgttg
    46321 cagatatact aaatgtaaag ttgcctaaac atgggtcaag ctttaaagaa gatttatttt
    46381 aaactataaa atgataaaaa cttagcttaa taggatggag agattggaat gtttgaaaag
    46441 atagcacaaa gtagtaaatt tataaattct aaaagtgata taaaacctaa gattggtttg
    46501 atattaggtt ctggactagg tgatttagca aatgatatag aagaccctgt cgcaattaaa
    46561 tatagtgaaa ttccaaactt ccctgtatca acagtagcag gacatgctgg acaacttgta
    46621 attggaaaac ttgaaggaaa agaagttatt gctatgcaag gtagatttca ttactatgaa
    46681 ggatattctc agaaagaagc tacttttcca gtaagggtta tgaaagaatt aggagtcgag
    46741 attcttatag taactaacgc tgctggaggc gtaaataaag aatttaaagc aggagatttg
    46801 atgcttataa gagaccatat aaactttagt ggaagtaatc cattagtagg aaaaaatgat
    46861 gacagatttg gtgctagatt tccagatatg tcagatgcat attcttctaa atatgtagat
    46921 gtagttaaaa actgcgctaa agaatgcaat atagatgtca aagaaggcgt ttatatgttt
    46981 tttagtggtc caaactatga aacaccagca gaagtgagaa tggcacaaat tttgggagct
    47041 gatgctgtag gtatgtctac tgttccagaa gtcatagtag cttctcactc taatatagga
    47101 gttattggta tatcttgtat aacaaatatg gctgctggca tcttaaatca accactaagt
    47161 catgaggaag taatagaaac tactcaaaaa gttaaatcag aatttatgaa tttagttaaa
    47221 acaacggtta aaaatttata agttaaagct tattatagat tttttgagga agtttataat
    47281 aaaatagtta atgtttaatt tgaaaagaat taataaaaag ctgtcttgat atagaaagtt
    47341 atttctaata aaggacagtt tttttataat actacaacat ttgaaagtgt tttaaagttt
    47401 aatatttatt taaattatta taggttagaa tggagatttc aattatgaga atgtatgata
    47461 ttataaaaaa aaagagagat aacctagagc ttagtaaaaa agagataaac tactttatag
    47521 aaaactattc aaagggagat atacctgact atcaagcttc tgcactatta atgtcgattt
    47581 atttaaataa gatgaataaa caggaaactg tacatcttac agaagcaatg atgaactctg
    47641 gagacatgat aaacctatct gagatagatg gaataaaggt agataagcat agtactggtg
    47701 gtgttggaga taagactacc atagctctta tacctttggt tgcttcatgt ggagcaccag
    47761 ttgcaaagat gtcaggtaga ggtcttggtc atactggtgg tactattgat aaattggagt
    47821 ctataccagg tttttcgaca gaaatggaga taagtaaatt tattgattct gtaaataagt
    47881 ctaaaatagc agtttgtggt caaagtgcaa aggtagcaat tgcagataag aaaatatatg
    47941 ctcttagaga tgttacagca actgttgata atatatcatt gatatcatca agtgtaatgt
    48001 ctaagaagtt agcttcagga gctgatgcaa ttgtgctaga tgtaaaaact ggtgatggag
    48061 catttatgaa gacattagaa gagtcttttg agttagctaa atctatggtt gatataggta
    48121 ctggtatgaa tagagataca atagggattg taacagatat ggatgaacct ttaggttttg
    48181 cagtgggaaa ctctttagaa gttatagaag caattgagac attaaaagga aatggaccta
    48241 aagaccttgt tgagctatgc gaagtattag gagcatatat gttggtttta tcaaaggtag
    48301 cacatgattt tgaagatggt gttgaaaaaa taagagaatc aataaaatct ggtactgcta
    48361 tacaaaaact caaagagttt atagagaatc aaggtggtaa taaagatgta gtagatgatt
    48421 attctttatt caaaaaatca aagtataaat atccagttaa atcaactaaa tcaggttata
    48481 taagcaaaat taaagctgaa gatgttggtg tatcggctat gattcttgga gcaggtagag
    48541 aaactaaaga tgatatttta gatttatcag cagggataat tttagaaaag aaagtaggag
    48601 attatgtaaa tgaaggtgaa gttttagctt acatgtatta taatgatgaa caaaagcttt
    48661 cattggcaaa gggaagattt attgattcct actctattgt agattcaaaa atagaaaaaa
    48721 ataaattaat atatggtatt gtaactaaag aagagattaa aaagttttag aaaattttag
    48781 aaaagtgatt ataaaaggcc taacatgtca aaaataatgt taggtttttt attttttgat
    48841 agactataaa ttata
//
LOCUS       DAMKMI010000013        47763 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMKMI010000013 DAMKMI010000000
VERSION     DAMKMI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.013"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            35..916
                     /locus_tag="PC429_000727"
     CDS             35..916
                     /locus_tag="PC429_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF3846245.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSIKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(1229..2008)
                     /gene="hisJ"
                     /locus_tag="PC429_000728"
     CDS             complement(1229..2008)
                     /gene="hisJ"
                     /locus_tag="PC429_000728"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF3846246.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLILGSDSHCAEEINKYDIV"
     gene            complement(2554..2904)
                     /locus_tag="PC429_000729"
     CDS             complement(2554..2904)
                     /locus_tag="PC429_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF3846247.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2920..3336)
                     /locus_tag="PC429_000730"
     CDS             complement(2920..3336)
                     /locus_tag="PC429_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF3846248.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3355..3546)
                     /locus_tag="PC429_000731"
     CDS             complement(3355..3546)
                     /locus_tag="PC429_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF3846249.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3697..4512)
                     /locus_tag="PC429_000732"
     CDS             complement(3697..4512)
                     /locus_tag="PC429_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF3846250.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(4559..5497)
                     /locus_tag="PC429_000733"
     CDS             complement(4559..5497)
                     /locus_tag="PC429_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3846251.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5494..6366)
                     /locus_tag="PC429_000734"
     CDS             complement(5494..6366)
                     /locus_tag="PC429_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3846252.1"
                     /translation="MNKNLSEKMEIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(6391..8319)
                     /locus_tag="PC429_000735"
     CDS             complement(6391..8319)
                     /locus_tag="PC429_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF3846253.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8431..8808)
                     /gene="gcvH"
                     /locus_tag="PC429_000736"
     CDS             complement(8431..8808)
                     /gene="gcvH"
                     /locus_tag="PC429_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF3846254.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8895..11021)
                     /gene="cdhC"
                     /locus_tag="PC429_000737"
     CDS             complement(8895..11021)
                     /gene="cdhC"
                     /locus_tag="PC429_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF3846255.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(11078..11884)
                     /locus_tag="PC429_000738"
     CDS             complement(11078..11884)
                     /locus_tag="PC429_000738"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF3846256.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11934..13301)
                     /locus_tag="PC429_000739"
     CDS             complement(11934..13301)
                     /locus_tag="PC429_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF3846257.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13332..14276)
                     /locus_tag="PC429_000740"
     CDS             complement(13332..14276)
                     /locus_tag="PC429_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF3846258.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14296..15066)
                     /locus_tag="PC429_000741"
     CDS             complement(14296..15066)
                     /locus_tag="PC429_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3846259.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(15122..16507)
                     /gene="lpdA"
                     /locus_tag="PC429_000742"
     CDS             complement(15122..16507)
                     /gene="lpdA"
                     /locus_tag="PC429_000742"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF3846260.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16551..17432)
                     /locus_tag="PC429_000743"
     CDS             complement(16551..17432)
                     /locus_tag="PC429_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF3846261.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17468..18142)
                     /locus_tag="PC429_000744"
     CDS             complement(17468..18142)
                     /locus_tag="PC429_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF3846262.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(18179..19051)
                     /locus_tag="PC429_000745"
     CDS             complement(18179..19051)
                     /locus_tag="PC429_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF3846263.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLIKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(19143..19775)
                     /locus_tag="PC429_000746"
     CDS             complement(19143..19775)
                     /locus_tag="PC429_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF3846264.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(19880..21556)
                     /locus_tag="PC429_000747"
     CDS             complement(19880..21556)
                     /locus_tag="PC429_000747"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF3846265.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21805..22584)
                     /locus_tag="PC429_000748"
     CDS             complement(21805..22584)
                     /locus_tag="PC429_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3846266.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(22894..24813)
                     /gene="cooS"
                     /locus_tag="PC429_000749"
     CDS             complement(22894..24813)
                     /gene="cooS"
                     /locus_tag="PC429_000749"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF3846267.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(25420..26331)
                     /locus_tag="PC429_000750"
     CDS             complement(25420..26331)
                     /locus_tag="PC429_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF3846268.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(26447..27025)
                     /locus_tag="PC429_000751"
     CDS             complement(26447..27025)
                     /locus_tag="PC429_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF3846269.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRAIDEIPE"
     gene            27251..28174
                     /locus_tag="PC429_000752"
     CDS             27251..28174
                     /locus_tag="PC429_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3846270.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(28346..29488)
                     /locus_tag="PC429_000753"
     CDS             complement(28346..29488)
                     /locus_tag="PC429_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3846271.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(29506..31260)
                     /locus_tag="PC429_000754"
     CDS             complement(29506..31260)
                     /locus_tag="PC429_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF3846272.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKKSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            31396..31602
                     /locus_tag="PC429_000755"
     CDS             31396..31602
                     /locus_tag="PC429_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846273.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(31607..32530)
                     /locus_tag="PC429_000756"
     CDS             complement(31607..32530)
                     /locus_tag="PC429_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846274.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(32784..34484)
                     /locus_tag="PC429_000757"
     CDS             complement(32784..34484)
                     /locus_tag="PC429_000757"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF3846275.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(34894..35307)
                     /locus_tag="PC429_000758"
     CDS             complement(34894..35307)
                     /locus_tag="PC429_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF3846276.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(35373..37751)
                     /locus_tag="PC429_000759"
     CDS             complement(35373..37751)
                     /locus_tag="PC429_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF3846277.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            38070..39521
                     /locus_tag="PC429_000760"
     CDS             38070..39521
                     /locus_tag="PC429_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF3846278.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKEVKGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(39725..42238)
                     /locus_tag="PC429_000761"
     CDS             complement(39725..42238)
                     /locus_tag="PC429_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF3846279.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGEINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPIEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIAMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTSQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKALDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(42461..43282)
                     /locus_tag="PC429_000762"
     CDS             complement(42461..43282)
                     /locus_tag="PC429_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3846280.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(43800..44276)
                     /locus_tag="PC429_000763"
     CDS             complement(43800..44276)
                     /locus_tag="PC429_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF3846281.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(44311..44889)
                     /locus_tag="PC429_000764"
     CDS             complement(44311..44889)
                     /locus_tag="PC429_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF3846282.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGNEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(44993..47593)
                     /locus_tag="PC429_000765"
     CDS             complement(44993..47593)
                     /locus_tag="PC429_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF3846283.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTAPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLGTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHSNTAVTDKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNVFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        14625 a         9142 c         5366 g        18630 t
ORIGIN      
        1 gtcgacacaa taaatttaaa aaggagaatt aaatatgaaa aaaagattaa taataatgat
       61 attgtctgtt gtcttagtac tatcaagtat acttacgata tttgcatatt caaatattaa
      121 atataataat aataaattga tatattctaa tatgatagat aaaaaaactc aaaatagtgt
      181 aaaggaaatc ctaaaagaaa ataaaattaa tgaaaaagac atagatacat ttattaaagc
      241 agtcaacaat tataataaat tgcaagttaa aatacttcaa aataatataa atatatctaa
      301 atctggatat tcttcaatca atgcaaaaca agttccatat aacctagaaa aattgcaaga
      361 taattgggtg aaaaaattcc ctgattatat ggatgtaaat tgtagaataa ctgcttttag
      421 actttttaaa gattttataa attcaaataa gaaatttact ggagactcta ttgatttgaa
      481 tgtcgacttg gacactataa tgaataataa agatgctaaa tttagcatca aagatgtaga
      541 aaaatttatc aacttctttt ctgcaatacc agctaaagat acagatgata caatcaaaat
      601 ttcagaacaa ataaaaaatg aatggaaaaa aagaaaaata tcttttaaaa ataataaaaa
      661 tatatctatt ataaatggat ttcttcgtta tccagaaact aaaaacgtat ttataggtca
      721 tacaggcata tgtattaaaa ctaaaaatgg cattctattt ttagaaaaat acggtgttac
      781 atcaccttat caagttacta aatttaaaaa taaagaagat gtaaaaaatt atatgtttaa
      841 tagattgaag atgtcagaag gtgaaattga attacctgac cctataataa tggaaaacga
      901 taaacttatg aaataataac tttaaacgca aaataatatt tataattact aagtcaatat
      961 aatagagaag gtctatagat ttgtgtaaat acaagcttat ctacaatctt atataagaat
     1021 atttatataa ataagataga ttaattctaa gtcctattat tctattgaat tttattatta
     1081 ttttatttag attctattgt tattttactt agaacttatt attattttgt ttaagttcta
     1141 ttactatttt caatcatata aatttacaaa agactgtaca taaatttatt ttatacaaac
     1201 ttatctacag tcttttgtgt gaatatatct aaacaatatc gtatttattt atttcctctg
     1261 cacagtgaga atcagaacct aaaattaatg gaatatcgta tttttttacc aactctaata
     1321 agtatccatc tatataaggc tctttgcagt actcttttct atttccagaa acattataat
     1381 caagttcata acctttttct ttaattaact ttattaactc ttcaagaatt gtatttcctt
     1441 tataatcgta tggaaatacc tgattaaact ttctaactaa atttaaatga cctattcttt
     1501 ttggtttatg tatacctaaa tctgaattta cagcttttat caatgtttca taatacttat
     1561 tataaacagc ttcaatactt cctaatttgt cgattaaatt ttgaaactcc tctacactgt
     1621 aatctacaca ataataatca tcatcaatct taattatatg tactgataaa agagaatcct
     1681 ctaattcctt tccatattta tttaaattct cctttatccc ttcttcgtat ccttctataa
     1741 aatccacttc cgacccaaca tttatcttga ttttaccctc atacttttct ttcaacttct
     1801 tgacatcatt aaaatacttt ggtaaatctt caatactcat acaactatca ttttcaggag
     1861 cagggtctct aaatccattt gcatacggaa aatgttctgt aaatgtcatt tctgttattc
     1921 caacatccat tgctctttgt acatattttt caaaatcatc cttacttcca tgaggacaat
     1981 aaggtgaatg tatatgacca tctttcataa ataattcact ccttttttaa agaaaaaatc
     2041 taatataaat gcataaatct tttaacttca aaataatatt tttaatcatg ctaattttta
     2101 ttatatctaa tattagagta attttaaact gccttttaag tatcttatca ttagtactat
     2161 tttcaactta acattaatat tattttttaa cttaacatta atactatctt taaatagtaa
     2221 tactcaatat ataattactt aacataaaat aactcaaatt gcttgatgag gcttttgcag
     2281 tatttaaaga atatataatt atttaacatt aaataatcca atcttgtaat aaaatttact
     2341 cattaaagta tattacagaa aatacctcaa aataaacttt ttagttcatg atatactctt
     2401 ttttgcatga aattagatat atttttatca tttcaacaat gaaagggagg cttatatcca
     2461 tctttagaca accccttaga taaaattttt aataattaaa catcacaaaa tataaaaatc
     2521 gcttatatca aacgtataag cgattaatta tatttacata tgttttttag cctctaacaa
     2581 cattttatta gcttcttcca tatactctat agccttttca atactctttg ctgtttcttc
     2641 ttttccaata gctctagcct tttcaaccca ctctctaaat ccttcttcat gagtttcatt
     2701 gtgatttatc caatgtacta aaagtatttt taaagttttt tcatccttag actctgatga
     2761 ttcctcatga ctatgttcgt ggtcgtggtc gtgagcatgg tcatgtggat gttcatgaca
     2821 tatatctcca tgagtatgtt cgtggcagtg gtcatggtca tgatgtccat gatgatatct
     2881 tacaatctct ttatcaaaaa tcattagtta ttccccctgt tattttatat taaactcttt
     2941 ttttataagg tttacagaat cttttacaag tagtgctaat ggtttacctt ctaatccaac
     3001 taactccaca ttttctggca taataggtaa aagtatctta agtgcctcac tgtctgcaat
     3061 agcttcactt atattacaag taacttctcc catcatagaa ttaggtatta ttacagaaat
     3121 aggtgcaact attatatttg cttttttagc tgatactact atagcatttt cccctgtagc
     3181 tcctttatta gcatgagctt tcatcataga actagttgct atagaattag tccctagtgc
     3241 ataaatttct acatatgtag gtaactcttc tctcaaagaa gaaactatct gggctccaat
     3301 acctccaccc attccatcta tcactgctat tatcatatga aattccccta ttaattaatt
     3361 ttcttgtatt attatcttat ggtccatcaa tcttatttct ttaattatcc catctattat
     3421 cttttgctct cctaataaat ccgttaaata aatctttcca tcacaagggt ctatattaac
     3481 cacattttcc ataacctttt ctaactcatt attactttta caaatgaaag ctgatgattc
     3541 acacatataa atctcctcct aatatgattt ttattaaaaa tcaaaaaaag attcccttag
     3601 gtatttcaat acaccaaaga aaccttcatg atttcagttt ttgtatatta ttatacctta
     3661 agtatatcac tttaatttat gtaaagtcaa ttaaagttaa cgctttttat tatactcttc
     3721 attcttgtat ttttcatata gttctttaac taattgcttc tcataatcag tcaaatcgcc
     3781 ctccacaaac tcaacattaa attgttcagc taatcccttt actatgtttt tctgtagaat
     3841 atctatgtct atttttctgc caatttcatt ttctattcca cttgcttttt tagctgtaaa
     3901 tttcatagct ctttctttca attctggtgt cttagttttt attaatttaa acaatttttc
     3961 tacatcaaaa tccagtatta ttgaaccatg ttgtaataaa acaccatctt ttctgctttg
     4021 agcacttcca attaccttct tattattaat agtaacttca tatgatgcat gtgcattaaa
     4081 acatgcagct gataaatttt ccctacttat tctctcgcct ctatttaaat tatctgtttc
     4141 tataccactt agattcaacc ctttaactag accttcactt atatatctat aagataggtt
     4201 gatgctctta tcgattaaag gattatcttc tcctataatt atactataag ttagctcatt
     4261 atcatgtaat acagctcttc cacctgtaat tcttctagta tagtctatat tcatacatct
     4321 acacttatct aaatctatct catcttccaa tttctgaaaa tatcctatac tcaaacaagc
     4381 tggttcccat gtataaaatc taagagttgg cttattatgt cccttcttat atgcagtaaa
     4441 tatagcttcg tcaattgcca tattcattgc tccttcatat gatttattat gtataactct
     4501 ccactgattc atgattatta cgcccccatt tttagtataa atatatccta gaaatcctct
     4561 atagatttat ttctcttatt gctaaatcta tctcactttg atttaaaggt cttgaatagt
     4621 tgtatatagt acttcctggt ctttcattat agtatggtct attacaatcc ttacaacctc
     4681 ttatttcaaa aggatagcct ttgtttatgt cttttaaaat atcattgtca agctttatac
     4741 tatttaagta tccattttta aacttaaaat actcttttgg ataaccttta tttatcatat
     4801 aagacattaa ctgtaccctt ctataacttt ctatgcttgg ttgatttatc ttttccattt
     4861 tagttcctct aactggagta aatgcaaata aactaatcgt tacatcattt tcctttaaat
     4921 aagtatataa attgtatata tcttcatgac tttcacccat accaactatt atatgtgtac
     4981 ttattttatt gggatatgat tttgacattt cagttataaa ttttaacttt tcatcatagt
     5041 tatttccttt tattttttca tatagttctt tatttgctgc atctattgct attcctattt
     5101 tatcaactcc tactgaaaaa aacttcttta tctgttcatc attttctaat tttgcagata
     5161 gtgaaattgg catgtctatc ttaccactta tatagtttat aaagtctaaa acagagttat
     5221 gtgcttcttc acttgccatt gattgaatac atattctctt tatatttttt cctttataag
     5281 cttttaaagc atctaatatt tcttcttttg aaaactctgg ccaaattact cttgataatt
     5341 tatcttttct agtactactt tctattgatt gagaacaaaa actgcattta tttatgcatt
     5401 tttctccaat cattatatat gctgtggtag gaggtatatc gctttttttg tttaatatcc
     5461 caagttctat tgctgtccca actgatagtc taatcataaa acgcaacact ccttacactc
     5521 atttattgtc atattcattt ctatagcctt attctttgca gaccttgatg gtaatactat
     5581 tctattgact ccacacatta tagataattg gtctagctct tttctataca ctcctcttgg
     5641 tctcatacaa cctagattaa tttcagtatc tggtaaatta attctagctt cacataaaat
     5701 atctgctacc ccttctaact caggaggttc tacattttca tactcagtcc cttttgttgg
     5761 aataaggact ataaatgtaa gcttattagg aggatttttt tgtaggtatt ctataatttc
     5821 atattcaccc tttatttgtc ctccttttaa accaatacat atatgtggtg ctacttctgt
     5881 atgttcttgt attgtattat agacttcaat ataatcttct ttactctttt tcatcttata
     5941 aacttctctt attgtctcat catcaaacac taaatcaaaa gaaactatat ctaaatactt
     6001 acacaactct acaatgctat ctttatccat aagacctaaa tgagcattca atctatatcc
     6061 ttgctctttt aagtttttaa tggtattaat atgagtattt atagggacat ctccctcata
     6121 actacaaccg ccactcaata gaaagctagt tatattcctt gattgtactt tttcctcata
     6181 ttcttctatt ggcaccatat tgtttaagta gtgaccgttg caatgggcac atttcaaact
     6241 acattgattg cttgtagtac taagagctag tgtctggtta ggataagcaa actctatttt
     6301 atttccaaaa ttctttaatt taatatcaaa tgctatttcc attttttcag atagattctt
     6361 attcatagtt tattccttct taagtacatt ttactcttct agtaatttct ttgctgttgg
     6421 gaactgctct atattcgtat gtggtaagaa acatgcagaa ataaattcat ccatataact
     6481 tggatataca ctaagttcaa cataagttat ttgattacct atttcttcaa gtttattttt
     6541 tgcatcttta cttataagag ctaagtaaga accaacaagt gaactattcc ctatataagt
     6601 aaatttttct ctatcaatat caggaagtaa ccctataatt attgagtttt ctatgtctag
     6661 gttgtttcct ataccacctg ctatataaac cctatctaat atactaaaat ccatacctaa
     6721 actatctaca agagtgtatg cgcctgaata tatagctcct ttagctctga tgaaattatc
     6781 tatatcaact tcattgacta ctatatcatt ttctaaatca aattcttctt taaatgctaa
     6841 tacatactct cctatctcat gctcattaaa tctaactctc ttattatcta aatccctgta
     6901 tattttacct cttctatcta ttaccccttt agtaatcatt tgacagatta aatcaattat
     6961 accagaacca catataccaa ctggtgcaca ttcatcaata atctttaatg ttggctctaa
     7021 agtttcttta tcaataatta ctttctctat tgctcctgct gatgctctca ttccacagct
     7081 tatccctcca ccttcaaaag caggtcctgc tgaacatgca cagctcatca tataatcttt
     7141 atttccaaat acaatctctc cattagttcc taaatctata aataaaacat tttcttcact
     7201 tgaccatatc cctgctgata atacacctgc tgttatatct cctcctacat aacttgcaac
     7261 agatggagct aagtacacat atgcactgtc atttacaaaa agaccaacat tttcacccat
     7321 aagttttgga gattttaaaa atggtggtat gtaaggttct tgtcttaaga agtctgtata
     7381 cacacccaag aataaacttg tcatagttgt attcccagca gccactaatg taactacatc
     7441 ttccttatct attccatttc tttcatatat tgtttttaat agtggattta tagtttcctc
     7501 aactatagct ttatgaagtg tctctaatcc atttttctta gttgaatata taattctatt
     7561 tataacatct gctccatatt ttatctgtgc atttccagaa gatgctttat ctactacctc
     7621 ttttgagtat aggtctacta aacaaacaac tactgaagtt gtaccaatat ctattgcaac
     7681 tccgtataag gaattttctt tatttccttg ttctatattt atgattgtaa gtttcttttg
     7741 tttttgaaca tatgttattg taaccttaaa atcactcttt ctaaatacag ttggcatttt
     7801 tctaagtata tccaatctaa aatctatttc attatatcct aggttatttc taacatatct
     7861 ttccaatcta tctacatcac ttatattatc atctaaattt ggttcttcca tttctatgta
     7921 tttctttttt atatttgttt taaattgaag gttatgttct tcaattattt ttttagctct
     7981 ttcaaaaatt tccctatcct cttttttatt actaccttct atcttcatac catgcataga
     8041 agatgatact ttagaaggaa cttcaatttc tatatctgat attacctttg tacaacaagc
     8101 tagtatataa ccttgctccc actcatcatc tgttatatgt cttgtttttt ctgtatcaac
     8161 ttttccattc aataatttta ctttacactt cccacaagac acgtttccat tacaaggagc
     8221 atctatgaat atatctgcat ttcttgcaac ctctagtaat atatccccct cattacaata
     8281 aacctcttta ttgtttggtg taaaacttac ttttatcatg ttatccctcc caatttgttc
     8341 tatatatgat aaaaggaaag tatagctaaa tttgcgtgca ctcaaatttt actatacttt
     8401 ccccaattat aataatatct taatctttaa ttattctaat ccagcctcgt attctttatc
     8461 acttaataac gtatcaagtt cagccacatc agctagtttt acttttaata tccagttttc
     8521 atatgggtct tcatttatac attctggctc atcttctaat ttttcattaa cttccaatac
     8581 ctcaccatta acaggagata ttaaatcaga agctacttta gaagactcaa ctactccaaa
     8641 atctactccc ttagttacag tatcttctac ttctggtgtt tcaacaaaca atatttctcc
     8701 taattgatct tgagcatagt ctgttatacc tatatataca acatctccat caattacttt
     8761 tacccactca tgatctttag agtattttaa ttctggtaat aatttcattt cgatagaccc
     8821 ccaatattat attattgatt aaattactta aatcatatta atattatatc atattatttt
     8881 aaattttcca tcaattacat tacactttcc atagctaatg ctggatgacc tttttcttct
     8941 aagaaagcta atactccttc agaatcgtca gctatagttt catcacaaac catatcacag
     9001 aagttttcta ttccagtcat ttctttaact gtagcattta atttatcagc tacataatct
     9061 tttaattctt ttggcatcca aactattctt tctggtccac cttctgcata agcgaatttt
     9121 ttagaagata tgaaatgtct tccgtgtccc ataaatcctg gagtttgaac tcctccacct
     9181 gtcatagaag caagttctcc gaaagtcata ccaactggag taacacttgc aaattctctg
     9241 ttaactacaa caaatccatt tgcttctggc attataccac agatacattc gaagcatcca
     9301 caagaagtca ttgggtcttc tagtatagag tataatgtaa cagattcaac tgcaccttga
     9361 gatatttggt ttacagtttc atttactgaa ttccatacac ctgttctatc atctaagcac
     9421 tcgccttttt ctattggttg acaaggacct gttgggtcta attctttagt agccttagca
     9481 tctaaccagc taactgctcc acaaagacca agtctttcag gagtaacaac acaaacgtga
     9541 gctggtgcaa atgattgaca taagttacaa gaatagaaag tatcaacact ttcatcaact
     9601 aatgaagcta atctttcatc tctagcattg tacttagcta ttgcttctcc ttttaattca
     9661 gaaacttttt cagcatctgt aattatagtt atttcacact tatcaactac tgattcaaat
     9721 tcatctaaca tcttagcata taaaacttct cctatatgct caagtctaaa tcctttatca
     9781 aatgcatctt tagagattct tacccaagcc atatctcttt gtccaacgtg cattacacct
     9841 tctatatagt ttaagaagta gtggaaacgt ctttcaagaa ctggctcgaa atcttcttgc
     9901 atatttttac cagctatttt aactattaca gcaagtggta atcttaatac accatctgca
     9961 tcaacttcat ctatatttgg tcctattatt tctatcttat ggtcttcaac ttctgttgct
    10021 tctttcttaa caacaagctc ccaagcttca gttctgtttc caccaaactc agcaaacata
    10081 tcgctctttc taattctctc accttcaaat gctgctgcaa atgaaactgg gataggtatt
    10141 tcagtaactt ttatttttat tcctctagct tctaatgaag tagctactat cttatcgtaa
    10201 tctttttgag ttaataggtt cattggaact tcaagaactg tttggtcagt tataactggg
    10261 aatcctaaag ctattgctcc agcacctgca gatacaacta attcacttaa tggtccaaat
    10321 gcattaacaa atgcaggaac tctattagct gtgtactcta ataatccgtt taaatcacca
    10381 ggagttaatc caccgaatat taatgctgct cttactgcaa cagatacaac gtgtataaca
    10441 gaagttacat catatcctag aggtataact cttagctcaa gacccatttt tactccagct
    10501 tctatagctt ggtctataac tttaccaact aagaaagtta gtaaaccttt agattggtaa
    10561 tcttttataa cttttgctgc tgattctgca tctggacact caccaagaac aactgcaaca
    10621 ccaggtatat ctcctgtaac taatggtaca ccaagtgatc tgattatagg gtcagttata
    10681 tgacctgcac atggctcact atatggagca tccatagttg aatattttaa tgcttcaatt
    10741 acttctgctg ctaaagctgt agctaaacca gcattaaaag catgttctag taaatgagtt
    10801 ctatttatta aagattttac tacttctaaa gcaccttcta agtcccctaa agtgttcatc
    10861 ttttgtcctg ttgcagcata tatacaaggt aatgaatatg ctgtatcagg gaaagcaact
    10921 ttatgctctt ttccattctt ttctatagct tcagctaaca tagcttgagc cgcacctaaa
    10981 gcttgttctg accctgtaaa gattatatta tatagattca ttctatttcc ccctttaata
    11041 tttacaccta ttttttatta actataccct aaaaacccta gaaagcaaat ccaccttcat
    11101 ttaactctaa gaaagagtct gcatataaag atgccccgtt gattatgtca caagatttta
    11161 cagttttcat taattctggg tcacatggat tcattatagc tgaactaaat ccattagcca
    11221 ttgccattgc taagaatgca ctatctaaaa caggtctaac atgcttagga catccattag
    11281 atacgttaga taatcctcct gtagttaaaa gtcccatctc agtcatcatt tttatagctt
    11341 ctaaaacttc tacttgtttt tcttgcatac cttttataac taagcataat gggtcaaata
    11401 gtatatcagt tggctccatt cctaacatta agcctttttc taatatttct tggcagtaag
    11461 ccatacgctc atcattatct cttgggatac cttcttttgc acataaacct ataaccattg
    11521 cttcgtattc agctgctata tctattaaat caaatcttga accagcatca gcagagttta
    11581 ttattggttt tgcattagtt ctatcataaa ctttaagtcc agcttcgata gcttttttat
    11641 tagctgtatc taatgctata ggaacattgt tgaactcaga ttgaagtaat ttgattcccc
    11701 atgtcattat ttcttcgcca tctctttcgg caggtcctat attaaaatct atatagtgtg
    11761 ctcctgcttc taattgctct tttgctcttt ttaagattgg agctgggtct ctttctgcta
    11821 atgcttttct tattgaaggt gatatacaat gtattctctc acctataatc ataaattttt
    11881 ccatttataa tgtccccctt taaatttaat attaatagtt agttaatatt ttattagttt
    11941 gtttggcatg ctttttcttg gtatcctttt aagaacttag gtaattccat agattcctct
    12001 ggtcctataa ctacattcca tcctggtaag ttatcttcta tatcaccttt aagtactgca
    12061 acttttcctg gaatgataag gtctttacaa ttagttactt cttctacctt actttcttta
    12121 atgaaagcag agattgagtt tccaccaaat ttaccagcag cccaagatgt aagaactgaa
    12181 tatccacctg catctggtat tactaaccat actggaactt ttgatctttc tatatcacca
    12241 gcaactatga agtaagttaa tgcaaagtca acagtaacta atactggaga attttcatct
    12301 gggttgttga ttggataaat ctttggttca actctcattg gtctttgtgg gtcagtgtat
    12361 atgttttgtc ttagtgcaaa taatggtaat gccttagcat aactaatatc atctattact
    12421 attatagaac cgtattttat agtgaatatt gatgataaag caacttccat catagggttt
    12481 gaattagata atctatttgc aaatactatt gaaggatatc caaatgttct atcttgctct
    12541 tttaaagctg ttcttctaac ttgtatagca ttagtgtaag tatcttttat gttttctcct
    12601 gttacatcta atactaattc tttatatcca gcagcttgaa ttaattcaac tgtttcatat
    12661 aattcttcta agctaccagc ttttacacct aatggtaaac ttgctccttt aactacttct
    12721 atcatatctt tgtagttctc tttagttgct ccatatacca ttggtctagc atcttttaat
    12781 acttcaacag cttctttaac tacttgtgca tcatcacaag ctaaaatata agctacttct
    12841 aatccactgc cttttatttt atctattaat tttaaatacg catctttatc tccacaatac
    12901 tctaaaacag ccatttcaac tttcatttgt tcaccaattc ttacatagtc aacttttttc
    12961 atgttagcta ttttagcatc tactgcttca tcactcatac aagtacagaa tgaaactgca
    13021 tatctatttc tacttactaa tgttttttca tgtctaaata atactgtttc tccacctagt
    13081 tcatactcag atgcaccagc accaactttt aaagctttca ttaaaggtgc agtagcctct
    13141 gataattttg ctatagcgtc atcagacata tgtgggcatt tacctacttc tacagctcct
    13201 gaagcaacct tcatagagaa agccatacaa gttgggaatc cacaatcctt acagtttttc
    13261 tttggtgtta atttaaatat atctaaagct tttaatgcca ttattttttc cccctaacct
    13321 taacaattta attaagctaa tgcgttaacc aattctttta tagtttctat tgattttgga
    13381 tgacgaagta taactgcgtt tgaaccacct actaaaacac ttgctgcagt tgaaacttcc
    13441 attgctatag ttctttcttc tggacaaccc caatctggct catcttctat tggagctata
    13501 gcttctttaa catgacctac ttcaaaagct actggtgtta tgataggcat ttgtaatgtc
    13561 ttatcatttt gaccaaatgc tgcaagtcta attctatcca tagtagatgc aacgtactca
    13621 tatccataac caactgctga acatccaaca ttcataacta tattttcacc tttaacgcct
    13681 agttgagtta acaatacgtt taattgttta gctaggttta tatcaacaga agattcagct
    13741 cctactttat gtgcataagc cataccagct gatgctccta ctcctttgta gttatcttct
    13801 actgctgaca taaataagca gttatgtcca tccaatgttt gagctaattt ttcaaataat
    13861 ttaccatctt tttcatgatt tcctgaacca gctacaacta aaggtaattt tattgcttca
    13921 attactgcct tagctacatc agcacattca tcaactgact tatctaatcc atttgggtca
    13981 gaaccatcaa attttaaaca aataaaatct gcttgtgtat tagcttcaac atatttagcc
    14041 cactctactg gacaattagc tacatctttg tataattcct tatatgaatc agtccagctt
    14101 tcaggataaa catctgatat ttgtatccct atttttggag aattacctac ttctccatca
    14161 aaactataaa aaggtaatac attttcccca cctaatttaa ttgctttttc ccctattcct
    14221 acttcaactt ctgatatttt tccagaatat ttttgagtag acattttaaa tgccatattt
    14281 ttttccccct taagcctatt ttaattctaa tttagcaaaa atttctttca tagccacttt
    14341 agattttgaa tcttcaggta aattaactaa aggaatacct gtagaatcat attcatatat
    14401 caattcatcc ataggtacaa caccgattag ctctaggtta tgcttttcaa tttcttcttt
    14461 aacaccatca ttcataaccc catttggaac tttatttaca atcaaaagta ttcttccaac
    14521 acttaacttt agttcctcag ctaaatctct tattctagca acagcttgta tgctacgtct
    14581 tgagcaatca ctaaccaata aaagtgtatc aacatgtcta gtgacttttc tacttagatg
    14641 ttccatacca gcttcgttgt ccatgactaa gtatttgtaa tgaccagata ttttgtttac
    14701 ttgttctcta agtatcccat ttacaaaaca gtaacatcct tcaccttctg acctacccat
    14761 tactaataag tcatatcctt cgccttcttc gattatagaa tttaatctaa attgaagata
    14821 ttgtgccttt gtcataccac cagggaatgc attgcctaac ttttctcttt gatttacttc
    14881 ttctcttatt tctcctattg ttgcttcaac ttcgatacct aatacttcat ttatgttagc
    14941 attggcatca gcatctacaa ctaatactgg tccctttttg tccttaacca aataatcaat
    15001 caaaagccct gtgagacttg ttttaccagt tccacctttt cctgcaacag ctatattgta
    15061 tcccatttta agacttcccc cttaaatcta gccacagagc cttatgtact ctgtctgtct
    15121 attataattt aggtacagaa tgaacacatt ctccatgtac atcatgaagt gcttccatta
    15181 gcccttcaga taaacttgga tgtggatgta ttgcatcccc tacttgttct actgttaatc
    15241 ctagatgaac agcaagtgac aactctgtca ataaatctgt agcatgaggt cctactactg
    15301 ctgcacctat tatcttatca gtttctttgt cagctataac ttttacaaat ccttggaaat
    15361 gtcctatagc ttgtgctttt ccaagacctc taaaatcaaa ttgacctaca ttgtattcta
    15421 caccttcagc ttcaagttgc ttttcagtct taccaacacc agcaacttca ggttcagtat
    15481 aaacacatct tggaattgct ctataatcta ctaccttagt cttacctaaa gcattttcaa
    15541 ctgctacgat accttcttta gaagcaacat gtgctaggaa aggagtatct attatatcgc
    15601 ctattgcgta tataccttct acattagttt caagatgttc attaactact acttttcctc
    15661 tttccatttc aattcctata tcttctactc cagaattgtc aaggttaggt cttctaccaa
    15721 cacatactaa tgcatattgt gcttctataa cctttccatt agaaagagtt gcaacagcct
    15781 taccatcaac tacttcacaa gtttgtactc caatacctgt aataacctta attttatctt
    15841 tcttaaattg tctaagtagt tgtttagcta catctttgtc ttcatttaat agtatttggt
    15901 caaccatctc aactatagtt acttctgttc ccaaggctct aaagaactgc cctatctcac
    15961 atcctataac tccaccacct actattaaca tagactcagg tatctcttca agtccaagaa
    16021 cttcatcact agttattaca acttttccat catatgggaa cattcttggt acaactggaa
    16081 cagaaccatt tgctagtatt atcttatctg cttttatagt ttctactgtt ccatcatctt
    16141 tagttacttc tatagtgttt ttatcaatta attttccaaa accattaact aagtttactc
    16201 ctcttttttc aaataagaac tctataccac ttattaattg gtttacaact ttattttttc
    16261 tttccattat ggcagtgaag tttggcttaa ctgtaccatc tatttctata ccaaaatcct
    16321 ttgcttcttt tacagtattc aatactcctg aagaagcaag aagtgcttta gttggtatac
    16381 atcctgcatt taagcaagtt cctccaactc ttcttttttc aacaactgtt acatctgcac
    16441 caagcataga agcttttata gctgctacat atcctcctgg tccaccacct actactacta
    16501 tcttcatctt taattccccc aaacctaata taatctacaa tcttgcaata ctataaacct
    16561 gcggcatcca gtatagcagg tatattctct tctgccccaa ttgccattat atgaacacca
    16621 tcacataaat cttcttcttt taattgtttg ataagttccc ctgccatttt tattccttca
    16681 cttacccagt tttcttttcc agctgctctt aatctttcga tttgttcatc tggaacaaat
    16741 attcctggta catttgcagt cataaattta gccattcctg gtgattttaa aggtactatt
    16801 cctgctaata ccttgcaatc catatccctt gttaattctc taaactttct cattgtattt
    16861 atgtcataaa gtgcttgtgt ttggaaaaat ttagcccctg cttttatttt ctttttcatt
    16921 tttaataatt gaacttctat tggagaatat tctggagtta ctgatgctcc cagataaaaa
    16981 tctggagaac cttttaactt attaccagcc atgtccgtac ctgccattaa agtctcagca
    17041 gtttgaagta tccccacaga atctaaatca aacactccct tagcttgtgg atggtctcct
    17101 acgctagtat ggtctcctgt tagtgccaac agattgttta tcccaaatac acctgctgac
    17161 aacatttctc cttgtatagc tattctattt ctatctctcc ctgttatttg aataactggc
    17221 tctaatccag catcttttaa taatttacat gtagcaagag aagttgctct cataactgcc
    17281 gattgaaagt ctgttacatt tgctgcatga actcttccaa ctaacggctt tgcacattct
    17341 attaaatgtg aaaggtcagt cccttttgga ggagccattt cagtagtaac tgcaaatttt
    17401 ccactttcta atgtttctct taataagctc atctctaatc ccccttttaa ccttctaaaa
    17461 aaaactttta tgaatttgct gctgcttctt tctttttctt tgcacttaaa cttcttggat
    17521 tattttgctt agaatagtct tttggtggtc ttatttctgc taagttatct aattgtccta
    17581 tagcttctag tctttcatat atctttatcc aagcacaatc attctctgag tttacttcac
    17641 acttaccatt tttagctcca ccacaagccc cattcataag acctttagca cacatagtta
    17701 ctggacatat cccacctgtc caaccaagtt cacagtctcc acaagcctta caggcttctt
    17761 catattctcc aactctttgt acttccccta taaacaaagt attgttggct ggataaactg
    17821 gcttatcttt taaattctta actattgtct gtgtaccatc tccacatgct aaagataaaa
    17881 ctgcatcagc ttccttagtc tcttccttta gagcctttaa atccttttta gactttaaaa
    17941 ggttacaagc agggtcaagc attacatatc ctaagatttt tttgccttca ccttcaagag
    18001 tttccttcat ttttaaaact tcttcttccc caccactttt acaagttgct gcacattgat
    18061 tacaaccaac taaaaccagc ttgtcaaaat cttttaagta ccctagcact tcttctaatg
    18121 gtttattttc agaaattatc atatctactt cccccctaaa ccttccacat atatatatct
    18181 atttattgtt ttgtaattta caagccttaa ctacatgttt tgcaagtata gatgttgtaa
    18241 ctgaaccaac ccccgctgga actggagtta tcattgatac tttgtctaaa actgcatttg
    18301 tatcaacatc tccacataaa tttccttctt catcaacatt tataccaacg tctataacta
    18361 ccgctccatc tttaacaaaa ctttcatcaa ccatcttagc tctaccaata gctgctatta
    18421 gcacgtctgc ctctgctgct acacctgata gatttttagt ttttgaatga catattgtaa
    18481 cagttgcatg ttcacttaat aaaagcattg atactggctt tccaactacc attgatcttc
    18541 caagaactgt taccttactt ccttttaaat caacattata gtgttttaat atctcaacta
    18601 ctgctgtagg tgtacatggt ggaaatccac ttttatcccc ttccattacc ttagcagagt
    18661 ttattggact aaaacaatct acgtcttttt ccggtgcaat aacatatttt ataacttctt
    18721 catttaagtg ttttggaaga ggtctaaaac ataatatacc attaacattt ttgtcatcat
    18781 taactctctt taatacatca atatattcct cttgtgttat atcctcagct aactctaaca
    18841 cttctgttgt tatacctatg ttttgacatc tctttagtgc tcctctttca taagataaat
    18901 catcacttct tgccccaact ctaactattg ttagtttagg atttatacct tctttaatta
    18961 gtaaatcgac ttcttttatt aattcttcac taatcttatc agctacaggt tttcctttta
    19021 taatttgtcc tttagttgac attccttcca tacattcaca actcccctac aagcaaactt
    19081 aaattaattt attctatata ccacatatta ataacatagt atctactttc gtcatttaaa
    19141 aattattttc caagagcttt ttcaactttg gcataaactt catcacaaat ttttacacca
    19201 tcttcaacta acttatctac ttctgttttt accttattaa catattcttt atcttgtata
    19261 gaatttatat ttataactac atttaattgt ccaccaagta tagcagctct taaacactga
    19321 actcctacac caacatcact tatagcaagt cttgaacctt tatcaactaa atcttcatgt
    19381 aacttaatag cttcatagca aactcttact atgtttaatg gtacttcaca agcaactttt
    19441 aatgcctttt ccattgtttc tgcttttatt cttttttctt cttctgtttc tttaggaagc
    19501 ccgtaagctt ttgataatgg taagaaacat tctgcatcat catctatcat ttttaataaa
    19561 tctttttcta actttcctgc cttacttaat atctctttta cactttcttc atactcagca
    19621 tacttcttct tccctattgt aaggttacat accatactac ctaaagccat acctattgca
    19681 cctactagtg cagcagctcc cccaccacct ggtactgctt ctttggaaga tagtacttct
    19741 acaaaatcaa cacaagtttt atctgctatt ttcatgataa tcctccttga aaggatgtag
    19801 gaaaatatct caaattgttc gagatatttt acgaactatt ttaagatatc tcctactctt
    19861 tatttataaa ttaatttcct tagaataatc cagctattac tccattttca tcaacatcta
    19921 ttttttctgc tgctggaact tttggtaatc ctggcatctt cattatttct ccagtaagtg
    19981 caactataaa tccagctcct gctgaaattg ttgcttgtct tacagttatt ctaaatcctg
    20041 ttggtctacc taatttagtt tggtcatcag ttaaagaata ttgagtttta gccatacata
    20101 ctggaacttt agtaaatcca agtttttcta agttagctat ttccttatca gcttcaggag
    20161 tataatctac accatctgct ccataaatct tagtagctat agcatttatc ttgtctttta
    20221 tagataaatc atcttcatag cagaatctaa agttattttc ttcttcatcc aataatctta
    20281 aaacttcttt agctactgca attccacctt ctccaccttt tgcccatact tcagaaagag
    20341 caacattaac acctaattcc ttacacttgt cttcaacaag tttaagttca gcttcagtat
    20401 caagtgggaa tctattgatt gcaacaactg ctggtaatcc atatacttga gttatgtttt
    20461 caacatgttt taataagttt ggaagtcctt tttcaagagc ttctaggttt tcgttattta
    20521 agtctgcttt agcaactcca ccattgtatt ttaatgctct tacagtagca actattataa
    20581 cagcatctgg ttttaagttt gccattctac attttatatc taagaatttt tctgctccaa
    20641 ggtcagcacc gaaaccagcc tcagttatta cataatctgc aaagtgcata gccattcttg
    20701 tagctataac agagttacat ccatgagcta tatttgcgaa tggtccaccg tgtacaaatg
    20761 atggtgttcc ttcaagagtt tgaactaggt ttggttttaa agcatctttt aataatgcag
    20821 ccatagcacc attagctttt aattgtctag ctgttacagg ttcaccttcg tagctgtatc
    20881 caactattat gtttcctaat ctttctttta aatcagatat atcacttgcc aaacagaacg
    20941 ctgccattat ttcagaagca acagttatat cgaatccatc ttgtctaact gctccatcac
    21001 cttttttacc cataccatca acgatatttc taagttgtct atcgttcatg tctacacatc
    21061 ttctccaagt tattttctta gggtctattc taagtgcatt tccttggtga atatggttat
    21121 caagcatagc agctaaaaga ttgttagcag ctcctatagc gtgaaaatct cctgtgaagt
    21181 gtaagtttat atcttccata ggaacaactt gtgcatatcc tccaccagct gctccacctt
    21241 taacaccaaa tactggtcct agagatggtt ctcttaaagc aactaataca tttttgtcta
    21301 gtttagcaaa tgcatcagca acacctatag tagtagtagt ttttccttca cctgctggag
    21361 ttgggtttat agctgtagtt agtattaact tagctttttt accagtctct ctttttagta
    21421 gattgtagtc aacttttgct ttgtatttac cataaagttc tacatcatct tcacctagtc
    21481 ctaatttttt tgcaacctct ctaatatctt gaggcttagc ttcttgagct atttcaatgt
    21541 cagatttaaa tcccataact gaatctcctc ccacaaccta aatagattta aaatataatt
    21601 tttgtaaaaa ttgtattaat atgtaaaaat tatattttat aagataatta tacatgtaaa
    21661 attacttaaa aactagctac taagctatta aaatattcaa acttaatagc tagttattta
    21721 tctaattact tacttattac ctatctattg cctatgtcta acctttaacc aacttaaaat
    21781 cccaaatcaa aatttctatc tattttactt atccttaagt gacattaatt tatcttttat
    21841 tgctttaatc tgttctttcg ttgtttcact agaatcatat ggagattgat tagctctatc
    21901 agaatcaata acatcttggt tataataaat aaatcctaaa gattctccat cttctaagtt
    21961 ttggattata aattcttcat cttccttatt tctaatttta tttcctacta caaatacttt
    22021 atttacacct atatcatgtc caagtttctt aacttttcta taagtctgta gacttctctc
    22081 tcctggttct acaactacta taaaagcatc tacaccttgg gctgttcctc ttcctaagtg
    22141 ttcaattccc gcttccatat ccataaccac tacatcttta ttttgtagta ttaaatgtga
    22201 gcagagtctc ttcaaaagaa catgctctgg acatacacat ccagtaccac cagaatctac
    22261 ggttcccaat gtaagtaatc ttactccatt gtattcttta cagaaatttt cagggatatc
    22321 gtctacttta ggattcatct taaacatttt tccaaatgaa cctacagaag cagctgttct
    22381 atctgaaact aattttttca tttctgatat aggtactatt gattcatata cttcttttgg
    22441 aaaacctagt gctaaagcta aattagcatc agggtcggca tcaacagcaa caactctgta
    22501 tccgtcttca gcaaacatcc tagagagcat tgaagaaaaa gtcgttttac ctactccacc
    22561 tttacctgtt attgctatct tcataatccc taccaccttt tattactatt tgctttttag
    22621 tagttaattt tacagttaaa tattttttat actctatatt tttatacact aaaaataaac
    22681 tacagctagt ccttacagga accaattaaa tttaccctac tagcttttca aagcagcatg
    22741 tatagtttta ttattcttta atattgttta tattttattt attcagtata ttcattatat
    22801 tttgatgttc tttactttaa tatttgactt tattcaactt tattcaacca gaaacttctt
    22861 ataaaaacgt ttattctata acttcactat tttttaagct tcagcctttt ctgccatttt
    22921 ttcttcaact aatgcttcaa agtgaacacg ttttttctct atatcttcta ttattgtagc
    22981 tgctaactct tttggatttg tatttacagt aaatgtagct ccaactttat cttttaattc
    23041 actagttaat atatccactg ctcttgaaga accagctatt ggaggcatta ttcctaaata
    23101 agtgtctata ccagaagcta caacatagca tcctatagca actgcttttt ctgacatcca
    23161 ctctggagct atacctacaa ctggaaggtc acacatatcc atatctaagt aattagctgc
    23221 tgctccaact aaatctagta tacggcttat atcaacacaa gaacccatat gtagtactgg
    23281 tgggattcct actaatttac agacagttgc aagaccttta cctgcatatt tttcagccgc
    23341 atcagattcc attaaaccaa acttagctgc tgctgaagct ccacaacctg tagtaactac
    23401 tattatgtcg tttttgatta attctttcat tattgttacg tgagcctcat ttgaaactcc
    23461 tttagcattg ttacatccaa ctacaccagc tgctcctctt aatactccag cttttaaaca
    23521 gtctgttaat ggcttaactg ttcctgctgg gtctatatga gagtttacaa ctctgtctaa
    23581 ttgatttact actgcttcta cactataccc aactgttgcg cctgatttta attcaggtat
    23641 aagtactttt gatttatctc tatttttaaa gtttaaaata gcttctttaa ctattgcttt
    23701 agcatcatca agtgcttgtt cttctctaaa ttcaatataa gttgaacctg ttatcttagc
    23761 ttttggagaa gttgttacaa atttagtatg gtaacaatct gcaactctag ctaatgcagg
    23821 gaatatacat tgaacgtcaa ctattacagc ttcaactgct ccagttatta ttgcaagttc
    23881 ttgttgatgg aagtctcctg ctatttttac accatgtctc attgtaatct cattacctgt
    23941 acaacacata cctgctaagt ttattccatc agcaccaact tctttagcta aagctactaa
    24001 gtctggctct tctgatgcta aaactatcat ttctgataat gctggctcat gtccatgtaa
    24061 tattatgtta actttatttt cttctaatac tgctaaattc gcctctgttc ttcttggcac
    24121 tggagttcca aataagatat cgcttagacg agtacctatc atagaaccac cccaaccatc
    24181 agccatagaa gttctaagac tcatgtgtat taaactatct atatcaccag tacatcctat
    24241 atgagtagag tgcattattg ttgcaatttc tctatctata gctcttggct ctatagcgta
    24301 ttctttccat attttttgtc ttggttctgg tgcatttttt ataaatctta atgtcccaaa
    24361 aggttttcca aattccatta atgcaatttc tgccacttca tgtgctacat catagatatc
    24421 tctaccttct gtctctacat cccattcttt agctaaagta attaacttag cttcgtctct
    24481 tattttatag tttccatttg gatcagctaa tgctaatgta tgtgctatat ctctagcatg
    24541 gtctgaatgg gcagctgtcc caccagcaac cattcttgca aagtttctac ctactatagt
    24601 atgctcatct gctccacata acccccttgg agtcttaggg cttattctac atggtcccat
    24661 tccacatatt ctacagcaaa caccttgaag accaaaacca cattgtgcct taaagccttc
    24721 ttttctatca tacattgttt ctacgccttc ttctctagct ttagctatca tcttctggct
    24781 gtttaaatca attgttaaca ttttttcatc catattccta tccccttttt ttgattttta
    24841 aaatccccca ggtgactgac ttcatgtcat aaaataataa aataaatgct ttctaagttg
    24901 attatatact aattttcagt gctttgcttt agatttaaat atttttttaa caaaattaga
    24961 taatacataa aaattttatt ttctacactt aatttaagtt gattttatcg cttgtttttg
    25021 gctatttgtt aatatctaaa cctacttttt tgatacttca gtttaattaa attatgtttt
    25081 gattttttta aatcaaattt gaaatttttc tagtcatttt tatattattg cagattgttt
    25141 gtgtatttat tatctttcac tactgtttgg acttattaca gatttttact ccctaaactt
    25201 aattagtaaa aatttcttag aatagcattt tttggactaa tttttttgaa tttttttatt
    25261 tttcctactt taatattaat tatagcttca aattgaacat ttgtatatat tcatttgaag
    25321 ctataattat tttttatata aaactttttt aatacagcat taatttagac attttgacta
    25381 ttttttctac tttaattcat ttatttcaag cgatttatcc tatttcatat ttttagctac
    25441 aatagcccca tatgcaatag aatataattt agattcatgt gaatcagctc ttgacactaa
    25501 aactatagga gcttttgcac ccataattat acctgctgat ttagagtctg ccatatatgt
    25561 aaaggctttc cctattccat taccaacctc tattgttgga accaatatga tatctacatc
    25621 tccagatact tcacttttaa acccttttat ctcactagct tcttttgaaa cagctaaatc
    25681 aaaagccaga ggaccttcta caattacatt ttcaccaaat tctccttctt tacaagcttt
    25741 tgctaacata tctgcatcta cagttgcttg catcttagga tttactttct ctttagctgc
    25801 caaacatgca acttttacag tttccattcc taaagctttt gcagctttta tagaattttt
    25861 taatatcttt gccttttgtt cataatcagg agctatattc atacctccat ctgtagttat
    25921 aagtaattta tggtattttt ctaactcata taccataaca tgacttaata aactatcagt
    25981 tctaagacca tagtctttat ttaacacttc ttttaatatg acagatgtat ctaataatcc
    26041 tttcattaca aagtcagctt ctttacttga tactaattta actgctattt ctgcacactc
    26101 tttgatatca tccttatcca ctattttgat accacttaaa tcaaatccta tttcttttga
    26161 tatttctctt attttgcctt catttccaac caatatagga gtaattatat ttctttctac
    26221 agcatccttt attgccatta aaacttcttt atcatgagct gctgctacag agagtacaat
    26281 tttctctcct cccttaagtt gttctaaaat atcatctaat ttttttatca aaagtaaccc
    26341 ctcccttata tatatttaac taaataatac catatttgct ataccttttc tcaatttatt
    26401 ttttatgtta taaagaattt atataactaa aagacagttt aatattttat tctgggattt
    26461 catcaatagc tcttaaaagc tttgcaggaa caccacctac aatagtattt tctgcaacat
    26521 tttttgttac aactgctcca gcagcgataa ttgcattatc tccaatagta actcctggaa
    26581 ctacagttac attagcacca atccatacat tttttcctat tgtgattgga gaaggataag
    26641 ttgtaccacg tttctctggt gcaataccat ggttaagtgt agccaatacc acattatgcc
    26701 caatttgcgt attatctcca atctcaatcc ccccctggtc ttgaaaatgg caactgctat
    26761 ttataaaaac attttttcct attgtgatgt tttttccaca atctgtataa aatggtggaa
    26821 acataaaaaa tgattcatct accttttttc caattaactc agaaaataaa ctacggactt
    26881 cttctggttc atgataagaa gaatttaact tacttgtgat tttcattgcc tcattactca
    26941 acttaaccat aaattcatgt atttctgatt tactagaaac aggttttctt tgattgatat
    27001 gtgttaaaaa ttcattgata ttcattttat acatcctttc aatatgtgct ttaatttatt
    27061 tgtacattct aaataatatt ttaataaaaa ttaatttaat ttatttttat taaaatatca
    27121 ttgcttcttt tagtgtaata tacaattata tctatatcaa atatctatat taaatactta
    27181 actatactta aaaagcatat aaattgatat aattatataa taaataagtt aatattgagg
    27241 agggataatt atggaaataa gagtattaag atatttttta gcaattgcaa gagaagaaag
    27301 tatatctggt gcggcaacct ttttacatat aacacaacca acactttcta gacaaattat
    27361 ggaacttgaa gaagagttgg gagttaaatt attggttcga ggaaatagaa aaatcacctt
    27421 aacaaatgaa ggtaggcttt tacataaaag agcagaggaa attatatcat tagttgataa
    27481 ggttgaatta gaatttgaca atcccaatga gattattagt ggtgatatat atataggaag
    27541 tggtgaaact gaagctatgc gacttatagc tagaactgcc catcattttc atcaaaaata
    27601 ccccaatgta agatatcacc tttttagtgg gaatgctgat gatgtgactg aaaaattaga
    27661 caagggactg cttgattttg gtattttaat tgaacctgca aatatacaga agtatgatta
    27721 tgcacaaatt cctgctactg atgtatgggg tttactaatg cgtaaagata gtgcccttgc
    27781 atcacaaagc acaataaatc caaaagattt atgcaatata cctcttatta tttcaagaca
    27841 gacacttgtt gataaagcaa tttctaaatg gcttcaaggc agtaatgaaa aactaaacat
    27901 tattgcaaca tacaatttag tttataacgc ttctcttttg gtagaagaag gtctgggcta
    27961 cgctctgtgt ttagatagac tcgtcaatac tacagaaaac agtaatcttt gttttagacc
    28021 tctatctcct cctttagaag ctaaattaaa tattatatgg aaaaaacatc aaatattttc
    28081 aaaggcagca gaaaaatttt tacttcaaat acaaaaagat ttttcttcta tcatgttgga
    28141 tacatctaag ttggatacac ctaaagaaaa ataaatcata ataaaaagtg agagtgtctc
    28201 aaaatgaact ttgcactgta taaggcactc ttttttatac aaagtaaaat atatgttcat
    28261 catttcagca ctaaaaaaga ggcttatctc tattttgaga tagccccact taaccaataa
    28321 aattaaatac aacttaacaa tatccttact tatttattcc aagttctctt agacaagcaa
    28381 tcgtaatttc atcttgtgga tttaattcat aagctctttc tatatagtaa gttgcatcat
    28441 caatatcacc attatttaaa taagccattc ctaaattgca aagtatttct gggtcttcct
    28501 ttatcttagc cgcttgttca aaatactcta ttgctttatc taaattattc ttataagcct
    28561 cacaaagccc caattccact atagtatcca cctgattagg ctttattatc aatatcttct
    28621 caagatacat cttagcctgc tctatttcgc ccataccctt atatccaagt gctatcataa
    28681 acagcaagtt ccaccaatcc atatgttctt cttctaatgg aagtaacttc tctaatcctt
    28741 ccttaaactt accttggaat acaagtgtat atccttcttc atattgaact ttaaagtcca
    28801 tcttgcccag attctcttgt acctcagcta ctaaatcagc atctaaccct agccttaacg
    28861 cttcttccca agtcagctta gtctttaaat attgaccttg attataataa tggtagccta
    28921 agtgataata acctaatgca aaattctcat caacatcaat taccttttca agcttagcca
    28981 tggcttcaag taagaatgca ttcattgcct tatcatccat atctttttga tactgtttag
    29041 ctatctcttg gcaaacaatc gcatagttat acattgcatc taaatcatct ggatacattg
    29101 ttataaatgc ttttatatat attaaagaat ctgtatgttc accaatctca aagtattttc
    29161 tcgacatata tcccatatac gatttcaaat ccaaatcaat atcttttgat aaagcattta
    29221 aaaacttctc atactctgca ttgtttttaa acttactatc tattccaatt atatatatca
    29281 ttgcatctgc tatagacatt gaatttaact tatcttgagc agtcttttct tttatccctt
    29341 ttactaatac ctcatttttt ataggtacat ctaatccacc ttttggcact tcatagcttt
    29401 ttaatttaaa ctctccacca tgttttatag ttataaaagc taattcttct gcttttttta
    29461 gcaaatattt ttctatctta aaatccattt tatcctccta aatacttaaa tctctacttt
    29521 atgagtttta gatttctcaa atattttttt atcatcatac tcaaataaga ctacattttc
    29581 tacttctctt atttctgtat ttatatcatt ttttaaatcc acaacattaa atctaaatgg
    29641 ctcaaaatga acatatttta ttatatgttt agctggttta tcaacaaaac taggtaaata
    29701 tgttgataag ttatcattat cttgtaaaaa tacatggcat ctattcttaa aatcatccac
    29761 atctagctta ttaaaaaact gtggtatatt tgatgtttta cccagtgata tatagtcata
    29821 ttttaatata tcgttgaata catcattatc tatgttgatt ttttcattat agaaatccat
    29881 caatatttta tataactgat tctttccttg tgccaaatca aagtagccat tttcatcaaa
    29941 atacttggca aaaacctcaa agaatttaaa agggcttttt ttataaaatc tgcctataat
    30001 atatctcata gataacacaa agtttttaga attgtagtat ctctccagta tatcttctat
    30061 atcctttagc ttcaaagttt cttcatagct tatataatta ttgtataaaa actcatatgg
    30121 agcataatcc ttatatttaa aaccatgctc atctgcaact tttctcatac ctgtcccttt
    30181 aatcatcttt aaaaagccta attgcagatg ctctatacct agattaaata catcattaaa
    30241 agaattttca aagctcttat aatcttcata aggcagtcct gcaattaagt ctaagtgttg
    30301 atgtatattt ctataagatg ctacagtttg tacaacatga gacaactttt taaaatcatc
    30361 ccttctacct actgcatcaa gtgttttttc attcgttgac tgaaccccta tttcaaattg
    30421 aaaaagacct tctttacaat cagccaaaaa ctcaagcatc ttatcatcta tcaaatacgc
    30481 tgtaacttca aaatgataag ttgtatatcc attatcattt ttcataagaa aatccatgat
    30541 ttccatagcc acttttctat ttgcattgaa tgtcctatct ataaacttta tttgtgatac
    30601 tcttgcatct attaaagctt ttaaatcact ctttatcctg tctaaactaa aataccttag
    30661 acctggaata gtagatgata aacagtattg gcagttaaaa gggcaacctc ttgatgattc
    30721 ataatacact attctatttt catattcttt tggattcaag ttttcataag gactaggaat
    30781 ctcatccaaa tcttgaagta attccatagg cttattcact attatctcat tgccttgtct
    30841 atatacaagt ccatttacat catttatttc catctttcct tgcaagtgaa gtactaaatc
    30901 tctaaaaatt aattctccct ctccataaag tatataatcc acaaaatcat actttttcat
    30961 tgcctcataa ctatcataac tcacttccgg tccgcccaaa gctattttag tattaggtct
    31021 aatttttttt agattatcac aaagtttaac tatatctccc acattccaga tatatgttga
    31081 gaataatatt atatcatatt catttttata tatatccttt aaaatatagt ccaaatcgtt
    31141 gtttatcgtg tattctttca tattaacttc tatcaaatct cttacaaatt cttttagata
    31201 tcttatagcc aaatttgtat gaataaactt ggaatttaat gttgttaata aaattttcaa
    31261 atatttctca cctcttttta agattatact gtctttttgt accccttata ggttaaaata
    31321 gtatatgtaa agtctaaata atatctatat aattaattta ttaatttcaa gtaatgtgag
    31381 gaaaggaagt tttttatgga attaattgaa aaagtatcta tcaactcttt atcaaaaaga
    31441 gatttactac ttattataaa gtctcttgaa tacactaatg aaaatactca aattagtgat
    31501 ttcattgacc ttaggaataa tatagtaaag gaattatgtt ttttaactga cactaaggaa
    31561 aaagattttt tagattatct agaaaaaaat agcacactct aataacctaa acctttttaa
    31621 actttaatat ataaatacct gatttagaat ttatttttat gcttaatata ttatcattaa
    31681 aggttttcga cataactgtt atttgattaa atatagggtc tactatagta ttaccttctt
    31741 caagtcttat aagacttctt gggtctatgc ttttattata tgccttaact ccaattttat
    31801 attttctcaa tttaacaaca tattcggaca tagaaaaaat aaaatctata ttatcttcat
    31861 ttaatttact atcttcaccc aaaatggata atatagattt ttttatgcta ttaattcctt
    31921 cattttctac agttctatct tccatatata catcatatag aacatttaaa acatttaata
    31981 aaatactttc ttcatcataa tcttcagatt tcaaatcaat gtacttatca attaattgta
    32041 ttgctttaat ccttgccatc tgaaacttta caacatctga tttgtctaat tctatagaaa
    32101 atcctattat cttgtcttta atactctgta gtaattcgac atactctatt ttactattat
    32161 ttataagatt gtgcataatg gaattataca ccacagaacc taataaataa tcaaataaaa
    32221 gttcttcttt tttaaaataa ataacatact tttttattag tttaataacc tcatcttcac
    32281 ttttttgaat attagtattt gataaaatta gtggtaataa aatatttaaa aaattgatat
    32341 ttcctatttt ttctccataa aatctatagt aaataagttc atctctcagt ctattttcgt
    32401 ctatatacac acatctttgc gttttatcat ctattaagga ttttgtattt attatagtta
    32461 aatctttaag agccctactt gcactactaa aagaagatgt gtagactttt tttagtaatt
    32521 cgtcattcat aaatatatcc tttctgaaaa attgaatgta aataaattgt tattaaaaaa
    32581 aaagagacag cccgtttaag aagctgtcat atataggggg atagtagtgt acaaaaatca
    32641 ttgtaatcta ctatctatat attattatac gatactactt ttttatatgt aaagcatcag
    32701 ttttcgacat ccctatattt tacagttata caaattgcgt actaagttca attttattct
    32761 ttttatttca acattttatc atgctacatt ctttcaggag cgtgcattcc caacaatttc
    32821 agagaatttt ctaaagtttg ttttgttgct aaaactaatg caagtcttgc ttttcttatt
    32881 tccaaatttt ctactaaaat tggattgtca tgataaaatt tattaaacgc ttgagctaaa
    32941 tctagcataa atctagtcac tatatgtggc tcatttttct ttagagcaag taaaatattc
    33001 ttattgaatg attctataac tctaagaact tctgcactat cttcatcagc taatagacta
    33061 taatctatat cagaagtcac ttctacttct gcttttctta aaactgcaca gcatctagca
    33121 tgtgtatact gaacatatgg tccagtctca ccatcaaaac taagcgttct ttcccaagag
    33181 aaggtatagt ctttaattct actattagat aactcttgga atactactgc cccaacacct
    33241 acttgcttag atatttcatc cacattttta gcatttgggt ttttagctag tataatttcc
    33301 ttagttttat ctatagcttg atttaaagca tcttctaaaa atactactct tccttttcta
    33361 gtcgacatag taccttcttc aagtgcaacc ataccaaatc caacatgaat taaatctttt
    33421 gcccaatcat atcccataag ctcaattacc ttaaaccatt gttggaaatg taagttttgt
    33481 tgagaaccaa ctacatatat acacttgtca aaatcataag tttcttttct atatattgca
    33541 gcagctaaat ctcttgtcat atataacgtt gaaccatcat tcttagtaat aagtgctggt
    33601 ggcattttat actcttctaa atcaacaatt cttgctcctt tagactcttt taaaagattt
    33661 ttctcttcta atagttcgat aactctgttc attttatcag aataaaaact ctctcctgct
    33721 aaagaatcaa attctatatc taaaagttta taaactctgt taaactcttt taaactctca
    33781 tttctaaacc attgccataa ttcttgtgct tcttcgtcac cattttctaa tttattaaac
    33841 catgctcttg cttcatcttc catttctggg tgttgttcag cttcatcatg gaatcttacg
    33901 tataatttta gtaattctgg aattggatta gactctacta cttctttttc tccccacttt
    33961 ttaaaggcca ctattaactt accaaactga gttccatagt cacctaaatg atttattctt
    34021 atagtcttgt atccttgcga atcatatatc ttatataaag cattacctat aactgtagtt
    34081 cttatgtgcc ctatatggaa aggttttgct atatttggag aagaatattc aactattaca
    34141 gttttatctt ttccaaaatc actatgtccg taattttctt tttcatctaa aactttttta
    34201 attacagttt ctgctaattg agatttattt acaaagaaat ttacatatcc gcctaattgt
    34261 attacttttg ttatctctcc acttggttct atactttccg ccaattcact tgctatcatg
    34321 tttggagctt ttctaaacac tttggctaat ttaaagcatg gaaaagcata gtcccccata
    34381 tccttatttg gtggaacttc tataagtgcc tctatctctt ctttcgataa atcttctatt
    34441 ttttctttaa ggcaatttga tattgccacc ttaaaatctt gcattctgtt gctcctctct
    34501 acattgttcc tggtttatat tgtgcttatc tctttatgtt aaatcaaatt tcgtgttttt
    34561 tcaagtattt cattacagtg taaaacactt tagtaattat gttttctgta tatattaaaa
    34621 atactataat ttcagttttt aatcacacta ttattgattt tttataagac ttttttctat
    34681 tttattgttt cttgtactat ccagttgact ttatatactt tttttaatct tttatctgtt
    34741 ttcatacatc aatgtcagct ttatatacat ttttttaatc ttttatctat tttcatacat
    34801 caatgtcagc tttatatact ttttttactt ttttatctat ttttatacat caatattagc
    34861 tttatatact ttttttactc ttttatctat tttctataaa tctttttcac tcttaacttt
    34921 tattccttct tcttctaata gccttgctgt tatacctttc cctggtatct ttttacctga
    34981 aaaactacca tcatatatta gattacatcc acaagagggg ctaccctctt ttagtattgc
    35041 ttcgctacat acatataaat ttgctatttt tagagtttca ttagcccctc ttacaaagtt
    35101 ttctgttaca tccttattat ctttagtaat tacttttcca tcaactatct cagcaggtgg
    35161 tcttggtgta gatagaccac ctaactgctc tgggcataca agtgtaaatt ctttgccttt
    35221 taaatattcc ttgacttttt cattatcatt attatcgcca ctatatttac aatttattcc
    35281 tattaaacat gcagatacca atatcataaa aatgtgctcc tatacaaatt atatatattc
    35341 tcatacaaat tactaatgta ttcttataca aattatttta atttaacaat tgttactcct
    35401 gctccacctt ctccatattg accacctctt tgtgatttta catgtctatt tcttcttaaa
    35461 atgtcttgta gccctgcttt taatactcct gtacctattc catgtattac tgttacagac
    35521 tctaaacctg ccacatatgc atcatcaaga tacttttcta cttccattat agcttcttct
    35581 aggtttaacc ctctaaggtc aacttcattt tttacactac cagattttga ccttataatg
    35641 tttctcgttg atttagttac atttgttgaa acatctttgc gtgttttttg taaagattta
    35701 aaaggtagag tcatcttcat tataccaatt tgcactactg cttcttttct ctttttatct
    35761 actgaaacta cactaccatt ttgatttaaa gttattacct taacttcttc acctggcttt
    35821 aagtctttta tctccttatt tgataccttt ggaactatca tactttttac tgttggctga
    35881 agacttccca tagagctagt caattctttt ctaagttctt ctatcttacg attcttttct
    35941 tttgaagctc tttcttgttc aagacttcta agttctttta ttataatatc tacttcttct
    36001 ttagcttgtc ttataattga aaatgcttct gatttagctt tttcaatcat cttatctctt
    36061 tgactgacca atttttcaag tttttcgtca tactctactt tcagtttttc aatctcttct
    36121 tttaatcttt cagcttcttc tctatcttct actgctttga ttctattctt ttcaacattc
    36181 tgtaacacat cttctaaagc tatgttttct gtatttatat attctttagc acgtgaaatc
    36241 acataatcac ttagcccaag ttttctagaa atctcaaacg catttgattt tccgggaaca
    36301 cctataagta acttatatgt tgggcttaaa gtttctatat caaattcaac tgctgcgttt
    36361 tctacacctg gtttagtaag agcataattt ttaagctcac tatagtgagt ggtagctata
    36421 catttagctc ctacagaatt tatatcttct aatactgcta ttgcaagggc agctccttca
    36481 acagggtctg ttcctgctcc taactcgtca aatataacta aagagtctgc tgttacgttt
    36541 tgtaatatag atactatatt cgtcatatga gatgaaaatg ttgatagact ctgttcaata
    36601 ctctgctcat ctcctatatc tgcaaataca ttatcatata cacacatact acttccataa
    36661 tcagcaggta tatgaagtcc actttgtgtc ataagtgcaa atagacctac agtttttatt
    36721 gtaacagtct taccacctgt gtttggacct gttatcacca atgtatggaa gtctcttcct
    36781 aaataaatgg tatttgctac taccttcttt ttatccagca gtggatgtct tccatttttt
    36841 atgtttaaat atttatcctc atttaaagtt ggttctattc ctctcatctg tatagaaagt
    36901 tttccctttg aaaatgcaaa gtccaatctt ccaagtatct cttgattaga gattaaatct
    36961 tcgctcactt ctccaaccat agcagaaagc tctgataaaa ttctttctat ttcttcttgc
    37021 tcacctagtt ttaactgtct caattcattg ttcatctcta ctatagtcat tggctctata
    37081 aaaagtgtcg caccagaaga tgactggtca tgcactatac cagctacttg cgacctatat
    37141 tcagatttta caggtactac aaatctatca cctcttagag aaattattgc atcttgtaag
    37201 tacttttgat aagtagttga acttattatt gaatttaatt ttgacctaat ggattgattt
    37261 ttttgggcta ttcttcttct gatatctcta agtgtacttg aagcattgtc agatatttct
    37321 acttcactaa ttatagcatt gtatatctgg tcttctatgt ccttgtatac atatagagaa
    37381 tttgatagag attgtattat tggataatta aaatcttctt catcactact agataagcta
    37441 tttcttaatc ttctagccac tcttaaggta tctgctatca taatcagact tcctgggtca
    37501 agagaagcac ctatataagc tctctttacc ttatcttcta tatcatgaat accttccaac
    37561 cctactgaac ctctctttat aataattgat tgagcttcac tagtttcttc tagcatagat
    37621 ttcacttcta caaaatcagt atttggtact aaattttcta tatattttag acctaaagaa
    37681 gatgaggctt tcttctttaa caaatctatt attttattgt attctagaac tcttaacgat
    37741 ttctcattca ttttctatat tcaccatcct cgatatttta acttttttat ttaattttaa
    37801 aaatgtatgt tttcaagcat ttccaataaa aactgcaagt tttatttctt tttcatttct
    37861 aaaattatta tattttttct cttttagata tattttacac atattttgta ttatactatg
    37921 tatgtagcta tctatatatt atacataaac atgctttatt tgtaatataa tctgttaaaa
    37981 aatcttcttt aatattttta aaggagttta ttttagctta taaaaaaaaa tagctactta
    38041 tatagataaa ttaaggagga acatgagtca tgggaaacgt attagaagga ttaaaaccag
    38101 aaagtgtttt taaaaacttt gagaaaatat ctcaaattcc tagaggttct ggaaatgaaa
    38161 aaggcataag tgatttttta cttagctttg gtaaaaattt aggtcttgaa actattcagg
    38221 atgaaagctt aaacataata ataagaaagc ctgctacaaa aggatatgaa aattgtccag
    38281 gtgtagttct tcaaggacat atggatatgg tttgcgaaaa agaaaagaat gttgaacatg
    38341 attttcttaa agaccctata aaattaagaa ttgatggtga catgatatat gctactggaa
    38401 ctactctagg agctgacaat ggtatagctg ttgctatggg tatggctata ttagaagata
    38461 atactttaga gcatcctgca ctagaagttt tagttactgt taatgaagaa gatggtatga
    38521 atggtgcaga tgcactagac cctagcttaa ttaaaggtca atatatccta aatatggact
    38581 ccgaggagga aggatactta ttagttagct gtgctggtgg taaaacttgt gttgttagtt
    38641 tacctgttga atacaaggaa gttaaaggtg acaaacaagg attattagtt gaggttactg
    38701 gactattagg tggacactct ggtatggaaa ttgttttaca aagagcaaat gctaacaaag
    38761 caatagctag agtattaagt gtattgaatg ttgattatga attagcttct gttgatggtg
    38821 gtactaagca caatgctata cctcgtgaag ctaaatgtgt aatagctgtt aataaagctg
    38881 atgttgaatc tgctaaaaaa caaataaatg acattttaac tgcatttaaa catgagttta
    38941 ctacttctga ccctggcatg acttactcag tagctgaaac ttctgtagac aaagttctta
    39001 ctaaagattg taaagaaaaa gttgttcaaa tgtcttgctt aactcctcat ggagttcaat
    39061 cagtaagtct tgatatagaa ggattagttg aaagttctac taacttcgct ataatcgaaa
    39121 ctaaagaatc tactattgaa tttttaactt ctgttagaag ttctgttatg tctattagag
    39181 atgaaatcgc tgatagaata agacttttgg ctcaagcttt aggtgctaat tacgacctta
    39241 tagctcaata cccagcttgg gaatttaaaa aaggctcaaa attagaaaaa atatgcagtg
    39301 aaacttatga gaaattaact ggtaaagttc ctactgttat ggctctacat gctggtcttg
    39361 agtgtggatt attacttgat aaacttcctc atgctgaagc tatctctata ggaccagata
    39421 tgtttgatgt tcatactcca aatgaacatg ttagtatccc ttctgtagct aatgtttggg
    39481 attatgttat tgaaatatta aaatctatga accaatatta aatttaagtt ttgattctaa
    39541 gtagatttta attttaagta atctatataa aattaactct gtaaattaat actacggtta
    39601 gtataatatt tactttcaat attaacttat agaaataaag cctaatgata gataataaca
    39661 tgtatcatta ggctttattg taatttaaat attattaagc tttattctgt tttaaaatta
    39721 attcttatac tattttaaat ctatttcttc cttgtctctt agaatcataa agagcattat
    39781 ctgctatttg atacagttca tcatactctt gaccaggctt tgctataact acccccatac
    39841 taatagataa actttctcca tttggcaact taatgagatg aatatgattg tatacatcat
    39901 tacatttatt aataagttct tccttattta ttatatcatt gatataaaca ctaaattcat
    39961 ctccacccaa acgacctact atatcatttt ttgcaaaact ttcatcaagt gcctttgcaa
    40021 catcaataag tactttatct cctaacaagt gaccataagt atcattaatc gatttaaaat
    40081 aatcaatatc tataatcaat aaagcaccat ttttttcagt tgataaattc aacaaatttt
    40141 gtgaagttaa ttttctaata tatgctgcat tataaatatt ggttaaacta tccctttgag
    40201 ccttatctaa caatctattc ttttcctctt tttcagaatc aatattacat attttcccga
    40261 ttacatacat tggtattttt ctatcattat aaattatttt tgaagtaact ctaatccaat
    40321 gaaaagtatc atcaagcata atagaataca tttcagttgt tccattttct tgtgatttta
    40381 ttacatcaaa aaacttttta ttatattctt tccttatttt atctctttca ttttctatat
    40441 tgccattttc actttcctta tcatttttat ccaaaccata ataagtctca ataccctttt
    40501 ctggcataga aattatcaat ttatcttgct tataatcata ctcaaaaaag tattcacttg
    40561 tcatttcata caactgagta tgttttttga gttctaaaga tatcttctcg ttcatacttg
    40621 tacgagtatg taaaccaaat gccataaatc caataataca aattaataaa ctggctacaa
    40681 taaaaatagt ctcaattgga ttctgttgca taatataaag caacgagaaa tcttgtctat
    40741 atgtagtatt ttggtagaca attgattgca ttttttctga tgaaatactt ataatagctt
    40801 tattaaatat attcaataat tcttgtttat cttttttaat tataccaaaa caaatactct
    40861 ttgctttata ggtttgagga actagacgca catttttata cttaggttga ttcatatagt
    40921 actgcataga ataaccttcg ccataagtgt aatctgcata gcctttatta acagcttcta
    40981 tacactcttc taaattatca taccaaacta tattctcttc aaactgacca taataattaa
    41041 tacctttggc tagagctaca cgttttccat ctaaactatt ctcatttaat ttattattaa
    41101 tcatcatagt atattgagta gaaagaaaag gccgagacat agctatatta tgttcacgag
    41161 agtattcata atcataaatc attcctgcta caatatcaat ttcacgcttt ttagtcatgt
    41221 tttctaaatc ttcttgagat tttacaggca caagttcaaa ttctaagcct gttttatcac
    41281 atatatgatt taacaaatca attgatatac ctcttaattc acctgtatct ttatctatat
    41341 tttgaaatgg tggtttgttt aaaagtactc ctacttttaa tttttctgtc tctttaatgt
    41401 aattagtttc ttcatcagat aaaaacattt gattattctc tggtgtaaag tatttttcat
    41461 ataaagtagt tgaaaaatat gggtctttct gttcaatatt attaattgct gagttaagct
    41521 tttgagttaa atttgtacta cctttaggag taatgaaata aaatggtttt gatgaaaatt
    41581 ttgctacagt acgtacacct tctattgggt tcatactcac attaagaagt acatctgctt
    41641 taccactctt tagtgcttct agttgctcat catttgtttt acaatatacc aattcaggtg
    41701 acataagatt cattttacaa tactcatcta attctttaag tctagtatca gaattttcta
    41761 ttacagcaat acggattttt ttcacatctt gtgaactcat tatgttatca atatctgtat
    41821 tttcatataa tgcttgtagt actgtatttg ctactccata attattccct gcataattat
    41881 atattttttg cattggttca ttgtataaca tacctcccat tatatcaatt tcaccatttt
    41941 ctaacatctt cattaatttt gtaatagatt catttatctc tccaggaact tgaacaaatt
    42001 cataattcca tccagtatac tgtgcaattt ctagtaaata ttcatatgta tacccaaaat
    42061 agttaccttg ctcatctatt tcagttaaac ctttttgaat aggataagca acacgaatgg
    42121 tacgattatc attacctata gcgttgatac caaaagaaaa tatgcaagaa gtgaatatag
    42181 atgtaattac aataattaat aaaagtaatt tttttaaatt cactacacaa tcctccatat
    42241 agttaagatt tacagtataa attactgcat aaacttgtag acatgtaaaa atagattagt
    42301 atataaaagg atatataaat ctacttagaa atttaaatat ccttaatata tacaatatat
    42361 ttaaattata actcatttat atgtctatta caacaaattt accctgctaa atacctactc
    42421 tatatcccat aattttacaa gttgtagtga agtttaagtt ctatctcacc tcaaatttag
    42481 cagtgtttct atttacagtt cttttatatt tcacatctgg ataacccaat ataaatgaag
    42541 tgataactgc ttcgttctcg ctcattccta acagttcttt tatctttggc tctacatttg
    42601 atgccataac aaaaaatcca ttataacaaa ttcctaatcc ttgagaatag gccataagtt
    42661 ccatattaga tgcagccaat cctccatcta cataagcact tccacctaga gacatatcac
    42721 ctacaactac cataagtagt ggggcatcat aaaataaaga atcatttccg ttctctttgt
    42781 atcttttgta cattttctta aatgtatttt tatagataga acgtacaggg tcattgtcat
    42841 cagtatctaa tgccaaatta taaagacctt gtattgccag ttctttcact tcttttagct
    42901 tttcttttac taatatgtat cttataggct gtctattacc acctgttggt gtgtatctac
    42961 cagcttccac tatgtttttt attttttctt cttctacctc tatatcttta tattgtctta
    43021 tacttcttct aaatttaata aaattcaata acttttcggg ttctaattca aaactttcac
    43081 gattgtattc tattacttca tttttatcaa atccttgcat ttctattgca tctgttgggc
    43141 aaattgccat acagtgacca cagttaatac agagaatatt tttaaccttt gctttatcat
    43201 ccttaacttt tattgcattt acaagacagt ctgattcaca catcccacag cctacacatt
    43261 tttctaaatt tatgtctatc attccaaatt attcctcctt ttaaaaaatt tactttagta
    43321 taaaacaaat agttgtctaa gtcaattttt atttttttaa caatatacta gccttaacta
    43381 cactttaatg gagaaaaata tacataaatt ggaacttaaa ttagaactta ttcaaagtaa
    43441 aagataaaag aagtagatgt ctaaaagtga actttttagt taattagata ttctttattg
    43501 tatgaaatca agtggatatt tatcatttca acaataaaaa aggcttacct ctattttgga
    43561 tatgtccctg tcaagtagac agactaaaaa aaggctatct ttatgcactg agatagcccc
    43621 ttttcccaac tactattgtt tatcttaata ttagtcaata aatttaattt gctataacct
    43681 gtaagttaca ttttaatttg atgtaacttt aaattacatt tttaagtaat atctttaaat
    43741 tatattctta aaatatatca ttaaactaca ttttaattaa tctaaaaact atatcttaat
    43801 taatctaaga aacttacatt ataatctttt agtttcccat tatcatatac aatctttgca
    43861 ctaaagatat ttccagtctt agcccaattt aagaacagcc tgtcaccttc ccacatattt
    43921 aaatcgaata ttttatcatt atcaacccaa attaaatttc cttctttaca atcatttaaa
    43981 tctttttcag ataattcacc ttcaaactca tctgagaaat acaaaaacat ataccaatct
    44041 tcttcatctt taaattttgg aaaagttatc aaccctttat atgacaatga tttaagagta
    44101 agaccagtct cttcttttac ttctctaatt acacattctt ctggagtctc tccttgctca
    44161 aactttcctc caactcctac atacttacct tcatgtatat catctttctt tttatttctg
    44221 tacaacatca aagtcttgtc atctttttct atataacaaa tagttgtaag tttcatgtat
    44281 ctcgccttgc tttctctttt atattctcaa ctaattattt tcagcacttt caagcacatc
    44341 acttatattc ttcattgcat taagagaaag ctctgcaaat tcatcaaatg gaatccctat
    44401 atcttctata cttctcattg catctctatt tacatttgaa gcaaatctcg tttctttcat
    44461 tcttttaata acagacttag tctttacact ggtaagtttt ttatctggat aaactaaagc
    44521 tatagcagtt ataaacccag ttatagggtc tgctgcatat attgcttttt ccattaggct
    44581 agttcttgta actcccattc catcatgatg accaagtatt gctctataca tttcttcgtt
    44641 accgaagttt tcttttttaa gcagttctac tgttgttgta gcatgtcctt cacatatttc
    44701 tcctggcttt cttccaacta tatctgaatc caaatcatgt aaaagacctg ccactgccca
    44761 ctcttcttct ttttctggct ctagtctttt ggctaattct ctcataacag cttcaacagc
    44821 ataacaatgt tttctcattc tatcagtttc taaatactta aataaaattt ctaaagcttc
    44881 ttctctcatt ttcaccaatc cccctgtttt ttcacatata tgatattttt atatattaat
    44941 acacataaat tcaagctttt atatatattc atgtatattt ttcatgttat atctattcta
    45001 cacctttata gtagtgtcct gcaactgttc caatttcatc taatttctta tacaagtctg
    45061 tagccttttt tccaagttta taattattac tcagtacctc aatgtgagct tcaataacct
    45121 ttttaaccat ctcactatct tcaactgtaa aatctatcct aaatacatct attccaattt
    45181 cctctaactc atatagattg tccaacatac aagtaacctt cgaattgtaa attgtacttc
    45241 tacagaatat atcttgtgtt atcctataac ttgcatccgt aaagtctctt aaagcataac
    45301 tactttcctt acatttagca actctcttgt cctttttaca atctctaaca actactccca
    45361 taggacagta ttctgtaacc atcataggag catatccata tattactgct tcaatttcta
    45421 catctgtata ttttaaagtt tgtttaattt catttaagtt tagttcttgt gacaaacaaa
    45481 ctgtacttgc tccctcttct ttgaagtgat ttatactttc actattgaat acatttaagt
    45541 agcaatctat atttaagtct tttttcttaa agtattccat agagccccag ttaccaattt
    45601 gaaccttatt aattcctttt atattatcca atatctcaaa ttgcttgtac tctttatttc
    45661 taacaatcct tggtgctgaa tagattaatt ttttgtcagt gactgctgta ttagagtggt
    45721 tcttattcaa agtatctatt ttacttgtat ttgacaaagt atctatgtta tttgtatttg
    45781 aacccattag ttctttagga gtacctaaag acgaagaagt atctaaatcc atagaaatac
    45841 ttgaatctat agaaatattt gaatctatag agatacttga gaccatttgt aaagcttttg
    45901 ataatgtatt tatatcttca taatagatta aatctacatt ataatttaat actgcttcta
    45961 attgctccaa gttcttaact ttaactctaa tcttaggagc tgtatcaagt tttttctgtt
    46021 tatttactct atttggctgg tattttaatc tattcttctt aaattttcta tcttttatct
    46081 ttattctttc ttcatctaaa agctcaatac aatcccttct catttgattt aacacactta
    46141 taggcatact tacaccttca tccaggtcaa tttctatctc ttttaactta tatggtgtat
    46201 ttccaagctt ttgaatttgt gtttctattt tttctttact taaagccacc tttatagctt
    46261 tttcaactat tttgtctcct tctaccaccg atttatttcc acaaaaatca cttataaaaa
    46321 gctctggctt tttacctagt ttaatactca cttttgcacg tatattagtt tttatgaact
    46381 ctttatcttg ctgaaatgta gcttgtactc tatccattaa ttcgctgtca gaagtcttat
    46441 atataagctg atttctcttt gcttctccaa caaaatctaa ttctatcaaa tcccctttat
    46501 gtgctattgt agttatctct ccatccttta ttattcttcc aatagtacct ccacctaagt
    46561 taattccatc acctttctta agcgtatttt ctagcattac tctaagtctt ttgttctttc
    46621 tattataatc taaaactttt ccaatatata gcccttgatt attaggtcta ttagagttca
    46681 ttatatcctt accaacttca cctaatatat atccttttgt aaactttcta ttaaatatgg
    46741 tatacaaatt atttattatt tcctcagaaa tacttatttt tttcttatct acaaattcat
    46801 taatcgcttc tctatatcct gaaaccacag tagctacata ctctggcttt ttcattctac
    46861 cttcaatttt taaagataat acacctgctt caattatttt atccaaatct tctatagtac
    46921 ttaaatctct aggactcaac aaataatctc cattactttt tactacttct cctgtattta
    46981 tatctatcaa ctcatacttt tgtctacatg gctgtgcaca cctacctcta tttccagacc
    47041 tattgcctat catactactc ataaggcatt gacccgagta acatacacat aaagctccat
    47101 gtacaaaaac ttctatatct acagttgtat tttcacaaat ccattttatc tcatcaacat
    47161 ttaattctct tgctaaaaca actctatcaa accctacact ctgaaggtat tttacatctt
    47221 ctaaggaatg tgctaccatt tgagtgcttg catgtagttc aaagtcaggt aatagttttt
    47281 ttataagtct tgccattcct atatcctgta ctattaaagc atcaatatct atatcgtata
    47341 aaaattttac atactctaca aaatcttcta tttcatactg ttttatcaat gtatttacag
    47401 taacaaagac ttgcactccc cttatatgag agtatttaac agcctcaatc agctcatttc
    47461 tatcaaagtt atttgcagat gctctagcac taaagtcttt accacctaaa tacacagcat
    47521 ttgctccgtt ttgaactgct gcttttaatg aatcaaaaga gcctactggt gcaagtaatt
    47581 ctattttttt catatttttc tcctctattt atacttgtta ggttaatata attatatcta
    47641 taacttaaat atttgtatta aatttatata tttcttacta tattttaaat tcttcaatca
    47701 atttatatat tagttattta tatttggttc atttgagtta acatatacat tatttactat
    47761 att
//
LOCUS       DAMKMI010000014        47613 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMKMI010000014 DAMKMI010000000
VERSION     DAMKMI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.014"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            125..313
                     /locus_tag="PC429_000766"
     CDS             125..313
                     /locus_tag="PC429_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF3846284.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(473..625)
                     /locus_tag="PC429_000767"
     CDS             complement(473..625)
                     /locus_tag="PC429_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF3846285.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(723..1199)
                     /locus_tag="PC429_000768"
     CDS             complement(723..1199)
                     /locus_tag="PC429_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF3846286.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1418..2179
                     /locus_tag="PC429_000769"
     CDS             1418..2179
                     /locus_tag="PC429_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3846287.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(2439..4367)
                     /locus_tag="PC429_000770"
     CDS             complement(2439..4367)
                     /locus_tag="PC429_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3846288.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            4601..5053
                     /locus_tag="PC429_000771"
     CDS             4601..5053
                     /locus_tag="PC429_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846289.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDASHLVKYITNKMKEE"
     gene            5056..5364
                     /locus_tag="PC429_000772"
     CDS             5056..5364
                     /locus_tag="PC429_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF3846290.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            5478..6584
                     /locus_tag="PC429_000773"
     CDS             5478..6584
                     /locus_tag="PC429_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF3846291.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            6659..9337
                     /gene="mngB"
                     /locus_tag="PC429_000774"
     CDS             6659..9337
                     /gene="mngB"
                     /locus_tag="PC429_000774"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF3846292.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(9654..11987)
                     /locus_tag="PC429_000775"
     CDS             complement(9654..11987)
                     /locus_tag="PC429_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3846293.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(11962..12678)
                     /locus_tag="PC429_000776"
     CDS             complement(11962..12678)
                     /locus_tag="PC429_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846294.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            complement(13093..13749)
                     /locus_tag="PC429_000777"
     CDS             complement(13093..13749)
                     /locus_tag="PC429_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3846295.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(13804..14433)
                     /locus_tag="PC429_000778"
     CDS             complement(13804..14433)
                     /locus_tag="PC429_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF3846296.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(14564..15205)
                     /locus_tag="PC429_000779"
     CDS             complement(14564..15205)
                     /locus_tag="PC429_000779"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF3846297.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(15214..15885)
                     /locus_tag="PC429_000780"
     CDS             complement(15214..15885)
                     /locus_tag="PC429_000780"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF3846298.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(15890..16774)
                     /gene="rsgA"
                     /locus_tag="PC429_000781"
     CDS             complement(15890..16774)
                     /gene="rsgA"
                     /locus_tag="PC429_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF3846299.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            complement(16872..17609)
                     /locus_tag="PC429_000782"
     CDS             complement(16872..17609)
                     /locus_tag="PC429_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF3846300.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(17717..19732)
                     /gene="pknB"
                     /locus_tag="PC429_000783"
     CDS             complement(17717..19732)
                     /gene="pknB"
                     /locus_tag="PC429_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF3846301.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(19749..20501)
                     /locus_tag="PC429_000784"
     CDS             complement(19749..20501)
                     /locus_tag="PC429_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF3846302.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(20521..21552)
                     /gene="rlmN"
                     /locus_tag="PC429_000785"
     CDS             complement(20521..21552)
                     /gene="rlmN"
                     /locus_tag="PC429_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF3846303.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(21560..22885)
                     /gene="rsmB"
                     /locus_tag="PC429_000786"
     CDS             complement(21560..22885)
                     /gene="rsmB"
                     /locus_tag="PC429_000786"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF3846304.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(22947..23648)
                     /locus_tag="PC429_000787"
     CDS             complement(22947..23648)
                     /locus_tag="PC429_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF3846305.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(23717..24469)
                     /locus_tag="PC429_000788"
     CDS             complement(23717..24469)
                     /locus_tag="PC429_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF3846306.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            complement(24479..25408)
                     /locus_tag="PC429_000789"
     CDS             complement(24479..25408)
                     /locus_tag="PC429_000789"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF3846307.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(25423..25863)
                     /gene="def"
                     /locus_tag="PC429_000790"
     CDS             complement(25423..25863)
                     /gene="def"
                     /locus_tag="PC429_000790"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF3846308.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            complement(25881..28406)
                     /gene="priA"
                     /locus_tag="PC429_000791"
     CDS             complement(25881..28406)
                     /gene="priA"
                     /locus_tag="PC429_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF3846309.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            complement(28645..29844)
                     /gene="coaBC"
                     /locus_tag="PC429_000792"
     CDS             complement(28645..29844)
                     /gene="coaBC"
                     /locus_tag="PC429_000792"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF3846310.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(29849..30115)
                     /locus_tag="PC429_000793"
     CDS             complement(29849..30115)
                     /locus_tag="PC429_000793"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF3846311.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(30118..30735)
                     /gene="gmk"
                     /locus_tag="PC429_000794"
     CDS             complement(30118..30735)
                     /gene="gmk"
                     /locus_tag="PC429_000794"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF3846312.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(30765..31646)
                     /locus_tag="PC429_000795"
     CDS             complement(30765..31646)
                     /locus_tag="PC429_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF3846313.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(31650..32480)
                     /locus_tag="PC429_000796"
     CDS             complement(31650..32480)
                     /locus_tag="PC429_000796"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF3846314.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(32767..33801)
                     /gene="ltaE"
                     /locus_tag="PC429_000797"
     CDS             complement(32767..33801)
                     /gene="ltaE"
                     /locus_tag="PC429_000797"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF3846315.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            34097..35872
                     /locus_tag="PC429_000798"
     CDS             34097..35872
                     /locus_tag="PC429_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF3846316.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            complement(36087..37739)
                     /locus_tag="PC429_000799"
     CDS             complement(36087..37739)
                     /locus_tag="PC429_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3846317.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(38038..39249)
                     /locus_tag="PC429_000800"
     CDS             complement(38038..39249)
                     /locus_tag="PC429_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF3846318.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            complement(39533..40066)
                     /gene="pyrR"
                     /locus_tag="PC429_000801"
     CDS             complement(39533..40066)
                     /gene="pyrR"
                     /locus_tag="PC429_000801"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF3846319.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            complement(40426..41373)
                     /locus_tag="PC429_000802"
     CDS             complement(40426..41373)
                     /locus_tag="PC429_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF3846320.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(41342..41788)
                     /gene="lspA"
                     /locus_tag="PC429_000803"
     CDS             complement(41342..41788)
                     /gene="lspA"
                     /locus_tag="PC429_000803"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF3846321.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(42098..42877)
                     /locus_tag="PC429_000804"
     CDS             complement(42098..42877)
                     /locus_tag="PC429_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3846322.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(42932..43414)
                     /locus_tag="PC429_000805"
     CDS             complement(42932..43414)
                     /locus_tag="PC429_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF3846323.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(43680..45101)
                     /locus_tag="PC429_000806"
     CDS             complement(43680..45101)
                     /locus_tag="PC429_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF3846324.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(45247..46017)
                     /locus_tag="PC429_000807"
     CDS             complement(45247..46017)
                     /locus_tag="PC429_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846325.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(46022..47224)
                     /locus_tag="PC429_000808"
     CDS             complement(46022..47224)
                     /locus_tag="PC429_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3846326.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT        15872 a         8160 c         5149 g        18432 t
ORIGIN      
        1 aataagaaat tcttcaatat gtgtaaggca aatatatatt tctattgcaa atttttgtta
       61 tttatgataa tataaatatg tttttagtct ttaaatagtt atcattttaa ggaggtgtgc
      121 gtagatggca aaagtatgta gcgtatgtgg taaaggtaag gtttctggaa accaagtatc
      181 acactcaaat aaacataata aaagaacatg gtcagctaac ttaagaagcg taagagcgat
      241 tatagatgga gctcctaaga gagtaaaagt ttgtactaga tgtttacgtt ctggtaaaat
      301 tgaaagagct tagtaaccac gtttaaaagc ctacttccgt aagtaggctt tttaatttaa
      361 tcatttttac tctttctcat aaattttagg attatacttg aaatccattt tggtaatttt
      421 attgttacta ctttcatcaa tactcacccc acactttata gttaaaacta ttttaacaaa
      481 aaaataataa tgctccacat ccaataagta ttaatgcggt aagtgctgac caaatccagc
      541 caggaagcaa cattgacaga attacagcca ttcctgttgc tagacaaagt attcctagca
      601 ataccttaga cttgttattt ctcatggaac acctccacat agaacctgta tctataaata
      661 ttttcatatt caagtttatg cgacagtttt ccattttaga ccaataaaca cattatgtaa
      721 ttttaatttt tcatactaga attattactg aactccgata tagattctac aatatccaaa
      781 attccttttt ttctacttaa cctttcaggt ccactatata aaacaactgg tataacttta
      841 ggaagtttaa aatttttatc ttctatttct tcaaggcttt ttttcataat aaaacttttc
      901 caaatttgtt ctctatacga tttcaatctt gggacaattt cagtatccat ttctgtttga
      961 aattctaaca ctatataatg tgatactttg ccacttttag aaaatacctt ataaattata
     1021 tcaacttctt ttcctttata ttcacatata aaagatgttt ctaactctat atttttaaga
     1081 gttaattctt ttgctattga gacacttgta aaattttgaa tcaaacttaa aaaactttct
     1141 ttatctgaat acattcttct gtactcttct cgtttttcat tgcttctttt taaattcata
     1201 cagttcctcc tagactatag accctctatt ttttagtaaa gatagtctat aaattttatt
     1261 ttttaatttt cattagtgag tattgacagt tgtgtgtaaa atatccagta aagatatttt
     1321 tttatatttt tattaaaaga tataaatttt tatattttaa tgtattgaca tttacgttgc
     1381 gtaaaagtgt atatttaatt tataggaggt actacttatg gaatatacag ttcaaaaatt
     1441 aagtaaacta gctggtataa gtactagaac acttagatat tatgatgaaa taggtcttct
     1501 taaaccactt aaaataaatt cttcaggtta tcgtatatat ggtcaaaatg aagttaataa
     1561 acttcaacaa atactttttt atagagaatt aggaattaac ttagaaaata taaaaaacat
     1621 tataaattca ccaaacttcg atagtttaag tgcgttaaaa gaacaccata ataaattact
     1681 agccaaaaga aagcaaattg atttactaat agaaaatgta actaaaacta tagctctgaa
     1741 ggagggaaaa tacactatga cagatataga aaaatttgaa ggttttaaag aaaagatgat
     1801 tgatgaaaat gaaaaaaatt atggaactga aattagagag aaatatggta aagatattat
     1861 agatacatca aataaaaaac ttaaaaatat gtctaagcaa gattatgaaa attggcaaaa
     1921 cctaaatatc gaaataatta gtaaacttaa aaaagctttt aaaactggtg atgcctctag
     1981 tgaactggct caggaagttg caaaattaca tcataaatgg ttgagttaca cttggaacac
     2041 atactctaaa gaagctcatt cctctctagc acagatgtat gtatatgatg aacgttttac
     2101 atcatattat gatagagaac aacctggatt agctaagttt ttaagggatg ctatagtaat
     2161 atatacaaga gataaataat ataaaataat aaaggtggtg tctcaaaatg aactttttag
     2221 ttcatgagac actctttttt gtatgaaatc agatatattt ttatcatttt atcaatgaaa
     2281 aacaggtttc tctctctatt ttgagacaga atctttattg tataataatt ataatcaata
     2341 caaaataata acaaattata caaaacttac ttatactatt tatgctaaaa acaaaaaata
     2401 ttttattaaa gtttttaaaa cttatgtaat ataaaaatct atactataga cttgattatg
     2461 gaaagaaaat tatcaaaatc attacaatta gatatactat ctctaatatc attgtcatga
     2521 attatcttta aaaggatatc ataaatggag tttattttta tataatcatc tttatttata
     2581 gccaacatca tgattaattt aacagtagaa tcttcactcc aatatattgg ttcatccaat
     2641 atggaaatac aaatcttagt tcttatagag cttaattcca taggatgagg tatagctaaa
     2701 aaatcatcta tattggtaga agataaactt tctctgtaaa atacagattc agcaaatgat
     2761 ggaaatacaa tttcactttg agttaatttg ttgcacatca actttataag ctcatcttta
     2821 tctttgattt taggattaac aataaataaa tttttatcaa agaaattgtc caaaagatta
     2881 ctataaatat gggaattatt agtgattgac ttagatatat tctctatatc tttttttaat
     2941 agttttaaat ccactaatac aataggaata ctatcatgat ttaaaggaat tgtcgatatt
     3001 attatatcta catctgacaa ttcactagct aaaaattgat taaatgatag acattgaagt
     3061 atatttattt tatcatgaaa aactttgttt aattgaacct ctaataacct acttgaacca
     3121 taccctgaac cacatactaa tactacgttt ttacacttaa tattactttg aaaaaatctc
     3181 tctattccag cacctatatg cagggataca tatccaattt catcttctgt taaagagtaa
     3241 atactatttt taaaaacttt ttctatagca tttaaagtta tttcaaatgc taaaggataa
     3301 ttactcttta ttgtatttat aaggggattc accttattta aattataaga tttagaattg
     3361 agaattgatt taaagtgcag tactaaatca tcaaataata cactatcatt tctaaggtca
     3421 aaagtatatt gattgttgat tacttctaaa atttcttcaa catactctat aattttagta
     3481 tctaccctat tggaaacgtt atccaaaaga gaacttttag ttataaaatg attatataga
     3541 taaactctgt ctgcctttga aataattata gtatatttac tctcaatata attaaatata
     3601 ttttcaacag ttgtattcga ttgaaaatct gttaaaacaa catctaaaac attatctaag
     3661 cagtaaccat caagtattct acttacagta atagctaaat ggataataaa gttttttaga
     3721 ttaaaatcta ggaaattgat attaaattta ttaatttcac ttaacactac cctttgaagc
     3781 tctattaaat ctacattatt aaataattct atttctctat ctgtaattcc aattatatag
     3841 ttttcaaaat tcctatttat catattatta gaaatacatc ttcgaatatc tatttcctta
     3901 ccttcaactt taactccata atatggctta gaaactaata ttaaattata ctttgacaaa
     3961 ataggcttta ttctttttat gtcatttagt attgtaaatt tactcacata caagctatta
     4021 gctaaatctt ctagttttat atatgtattg ttatagagaa tatattttaa aatatattga
     4081 tttctttcta taggagaatc tggtatttca cttgaatcca taatatcatc agatattaat
     4141 gctagatact gttggtataa tgctaaatta agaatatcta tataatatcc ttctcctttt
     4201 tttattttaa taatagcacc atttttctct aaagttccat ttatagaagt aatatcattt
     4261 cttatagttc tctcactcac ttttaaatga ttacttaaag ttttacaagt aattttatca
     4321 tcactttcat ttaataattt aaacaacgtg agcaatctct tgtgcataaa tatcccccct
     4381 tatagaattt cttctaaatt atactattta cactcatata atacaattac tctaataaaa
     4441 ttgtattata ataatttatt tccacttcta tatggaaatt agttattatc ataatataaa
     4501 tttccatgtc gaagtggaaa ttagttatat ttaatttaat taggataatg attacaatat
     4561 aaatataagt taagacatta tgaatggagt ggaaaaatat atggtactac aagaaaaata
     4621 tatatttata gataaagact taaaaagcaa agaagaagta ttaaatttta ttgcagatag
     4681 tgcttatata ctaggtatta caactaataa agaggctctt ttaaaagacc ttctaaaaag
     4741 agaaaatgaa tttccaacag gaataaaaga tggatttgcg atacctcata caaaaagtgt
     4801 aaatgtaaag gaagcttcca tactctatat taaaacaact aacgagttag agtggaaaac
     4861 tttagatgat agtaaggtta gatatgtatt tagtttgtta gccccacata aaaatgaagg
     4921 aaatatacat ttgaaaactc tatctaaatt agccacatgt ttaatggagg atgattttac
     4981 aaatgaagtt aaaaatgcta gtgatgcaag tcatttagta aaatatataa ctaataaaat
     5041 gaaggaggaa taattatgaa aatagtagca ataacatcat gtatagcagg attagcacac
     5101 acacctatgg cagcaaaagc tttagaacaa gctgcatcaa aaatgggtca tgaaatcaaa
     5161 gtagaacaac aaggtgctat gggacgagta aatactataa caaaagaaga agcacaagat
     5221 gcggacttta tacttatagc atctgaccaa actatatctg gaatggaaag atttgatggt
     5281 aaaaaggtga taaaagtcaa aataggtata gcgttaaaaa aaccagaagc tgtcttaaca
     5341 aagtgtatag aagctatttc ttaataaaaa aatctcactc taatatagaa tgagaaaaat
     5401 caaaaattaa aaaattgtct aatgtaatta taacatatta tcaaaaaata taacaaactt
     5461 atataggagg attaaaaatg agtaaaaaaa ataaatctct tttaagtgaa cttaagggac
     5521 acttaatgac aggtatctca tatatgttgc cacttgtaat aggctcatct ttagttgtag
     5581 ctatcccaaa gcttacagct ttagcaatgg gtataacaag tttagatgct tataaaaatg
     5641 tatctggttt ttatcatatt ctttacttaa tggaacaagt tggttggact ggcataggtc
     5701 tattaaatac ggtattagca ggatttatag cattttcaat aggtgagaaa ccagctatgg
     5761 gtgcaggttt tattggtggg ctcatagcaa gtaatactaa tgcaggattc ttaggagcag
     5821 taatagcagg tttctttgct gggtatgttt gtaagttttt aaagaaaaaa attaaaatat
     5881 ctgggtcagc atcaggaatg atgccactta taataatgcc acttataaca gttggattaa
     5941 ctggtttttt aatgagtgtt gtattagcag gacctttagg aaacataaat acttcattaa
     6001 atgcttgggt agctgaaatg tgtcaaaatg gaacaaattc ggtggtattg gctttaattt
     6061 taggcgcaat gataggattc gatttaggtg gaccagtaaa taaagcagct tggatggcag
     6121 gaaatgcatt attgttagag ggaatctatc taccagcgat attggtaaac tgcgcaatag
     6181 ttatacctcc actaggatat ggtttagcta cattcataag aaaatcaaag ttttccaatg
     6241 cattagctga aacaggaaaa ggcaatatag ttatgggtat aattggaata acagaaggtg
     6301 caattccttt cactttaaca aatccattaa aattagttcc tgttaatatg ataggatgcg
     6361 ctttgggtgt aggattatca gctttgttag gtgtacatgc tataatgcct ccagttggtg
     6421 gattatatgg atttatatca ataggttctg gatgggctta cttagtaggt gcaatatttg
     6481 gagctttagt aatagcaata ttatcaacaa ctttagtaga cttcaatgaa gatgaaacta
     6541 cggaaaatac agaatttgat gaaatagagt tagatataat gtaaattaaa aacttaattt
     6601 tagataattg agaattgagt gtactctcaa ttctcaatta aattttggga ggatttttat
     6661 gaaaaagaca aaagtacatg tagttccaca cagtcattgg gatagagaat ggtattttac
     6721 aacatcaagg tcaaaaatat atttaatgaa agattttcaa gatatattag acatattgga
     6781 agaaaaagaa gactttaaat actttactat ggatgcacaa gcttctctct tagatgatta
     6841 tttaaaatgg agacctcagg atgaatatag aataagagat ttagttcaaa gaagaagatt
     6901 agttattgga ccttggtata ctcaaacaga ccaattggtt atatcaggag aatctataat
     6961 tagaaacctt tactatggaa tagatagatg caatgaattt ggtgaaccta tgagggttgg
     7021 atatgtacca gattcttttg ggcaatcagc tcaaatgcca caaatataca gaggttttgg
     7081 aatagatagt tcattatttt ggcgtggagt ttctgatgat atggtagata caacagagtt
     7141 tatatggaaa ggttctgatg gttcaaaagt attagcagtt caaataccat ttggttatta
     7201 ctatggaggt aatataccag aagatgattc tgatttaaaa gaatatttaa aagatataat
     7261 cggaaaactt aaggttaaag cttctactaa taacgtatat tttccaaatg gatttgacca
     7321 agcaccaata agaaaaaacc ttcctgatat attaaaaaaa gctaatgaaa tagataatga
     7381 aaatgaatac gaaataagtg atatagaaaa ctatatagat tcagtaagaa atgaaagaaa
     7441 tgaatttaca gagcttaaag gtgaattttt aaatgctaaa catatgagaa tacacaaatc
     7501 catattctcc tcaaggtcag accttaaaat aatgaataac aaattagaaa attatattac
     7561 aaatgtactt gaaccattat taacattaag ttattcactt ggaaatgaat accctcacct
     7621 agttataaaa gatatatgga aacttatgtt tgaaaatgca gctcatgaca gcataggttc
     7681 ttgtaatagt gacacaacaa atgaggatgt atacacaaga tacaaacaag ccagagattt
     7741 gagtataaat ttattagatt tgcatatgag acttataagt actcaaatca aaaataataa
     7801 tgaagaaata acattaacag tattcaatgg attcccagaa gtcagaaatg aagttgtaga
     7861 atttgatact tatattccag gtcaagattt tgtgataaag aataaaaatg gaaaaattct
     7921 taattatata ataaaagaaa aagaagatat tacaaattat atattaactc aaacaataag
     7981 attaaaccca agtaaaaaaa tatatatacc caaaactgta tatagagcaa aaattgcact
     8041 ttcagtaaaa gatataccac caatgggata tactcaacta gtttttgaat tggatactaa
     8101 aaataaatgt gaaatagaaa caagtaattg caaggaaata gaaaacaaat actacaaaat
     8161 aacagccaat aaaaatggtt cattagaaat attagataaa gaatctaata agacttataa
     8221 aaaccaagct gtaattgaag acaatggaga tgatggagat tcatacaact attctcctcc
     8281 aagaaaagat atttatgttt catctttaga ttctgtatct tcagtagaag tgctaaaatc
     8341 aaacattcaa gaagaaatga tacttaaata ttcattgacc ataccaactt cactggaaga
     8401 aagggctata ggtaaagttt ctgtgaaaat gccagtaaca atgaaaatca ccttagaacc
     8461 taaagaacaa ataattaagt ttaatgttaa tataaaaaat aaccaagcac tatctcatag
     8521 agtaagagtt ttatttaata cagaaatagc atctaagttt tctatagcag atcaacaatt
     8581 tggaataata caaagaccaa cagtattaga aaaagacctt gcattgtgga aaagagacaa
     8641 ttactcatgg caagaaaaac ctataactat cgaaccaatg caaagttttg ttactttaga
     8701 ggatggacaa agaggaatag cattaataac tggttgtgta agagaatatc aaatagttgg
     8761 tgataattta gatacaatag cattaacact attcaggtca tttggatata tgggaagaga
     8821 aaatttactt tatagaccag gaagagcttc tggagaaaaa ataatagcaa caccagatgc
     8881 acaattactt aaagagttaa actttgactt tggtctttac atatataaca ataaatttga
     8941 tgaagcaaat gtagctaata cagctaagag attcttaaca cctatacaaa tatatgaata
     9001 tgctgatttt ttaaatggta gacttatatt tgcattcaat gacgaaaaac aaatatatga
     9061 aaatgaattt agtttgatga atgtaaataa ttcaaatttc acagtaagtg caataaagaa
     9121 agaagaaaaa ggagatggaa ttgtagttag aatcttcaat ggtatgaaaa atgaggatgc
     9181 taagggaagt cttaacatca ataaaaatgt caatgatgct tatagtgcaa tgctcgacga
     9241 aatctatgat aatactagca agcttaaagt aaatactaaa actgtagagg taaattcttt
     9301 atcacattgt aaagtacaaa caatagtaat taaataatat aaaatagctt atacaagatt
     9361 tcaaaataaa ttgctagtac tatgttaata gtaaagccag atattatgct atttataagc
     9421 tctgtaacac taaataacat tttgtaaaat aaaaaggtga accttgtata ttaggattca
     9481 ccttttttaa ataaatcaac attctgtttt aaaataacat ttataagctg ttttgaaata
     9541 atgcttacaa attattttaa aacaatacct ataaattttt atttaaagaa tacacataaa
     9601 tatttccaga ttcttttatt ctatagactt tataattatt aaagaagtct aatctaattt
     9661 ttgttaaaag taattgtaac tttgaataaa tcaccatcca cacttatatt catcttacca
     9721 ccctgtaatt ctataaaact ttttgctatc gctaaaccaa gaccagaacc ttctgtgtta
     9781 cgtgatttat ctcctcttac aaatctctcc attatatcat ctacactaaa atcaatctca
     9841 gatgcagaca tatttttaag cataatattt actttattat ctgtttctaa aatatcaata
     9901 tacactcttg aatttggcat tgcatatttg cttatattta ttataagatt ttcaaatact
     9961 ctaaatgtac gctgactatc taatggtaat attatttttt cattagaaaa gttattcttt
    10021 actattagag atgaatctgc aagtttgtca tctaattcta aaagtgtctg cttcattaga
    10081 gatacaatat ccacatttac tatattcaaa taaacatctc cactatttgc cttgcttact
    10141 tcaaacaaat cttctattag atgttgcaat ctttgtgact ttctgtctaa tgtatcaata
    10201 tacaacttac gattttcatc tgttatactt tcatctttta ataaatctat ataagtgatt
    10261 atagatgtta gaggtgtttt taagtcatgt gatacatttg atataagctc tgttttcatc
    10321 ttttgactct taacctcttc gtcaacagct tttttaaatc cagtttgaat atttcctaat
    10381 tcatctttta taggattaaa aaatcctaaa tctttttcca tatttacatc taagtttcca
    10441 tctgaaattt tctttgtaac atctaaaagt tgattatatt catatattgt ctttttagag
    10501 taatctgaca gtatcttaaa taatactact gaatatatta atgcgagtat tattccaaag
    10561 aaccacatac tacacattat aactataatc aataaattta ttcccaaaat tgtaactata
    10621 gttttatttg gcttttcttt cagatttatt gttctaattt gattaataac tgcttttcct
    10681 gcttttttta accatacaaa aaacattcct gtcacagtat gttttgtaaa atactctctt
    10741 attccaacat ttaaaatgtg ttttaaaagt attataaata caaatattat tgagaatatt
    10801 acaaaccaat aagttaaatt cattaaactt accaatatac tagccatttt agcacttatg
    10861 tcattgttta taaggtactc tacataattc ccactcaagg tattacttat tataacagta
    10921 ggatataaat ctacaagccc ctgtataata gcaagagcaa taaagagtat ctctaacggt
    10981 attcttgata atgtatcaaa tcctagcaac tctttagatt ttctatatgg gaatgctaaa
    11041 gctacaataa caactatagc taatataatt tttaaaatca ttccagaaat cttataataa
    11101 gaattatttt ctacattgct tatatatgaa attataccat catcaaaata agtatttcca
    11161 taaccatact ctacactatc acctttactt attactttag gtacagcata tactaatgtt
    11221 atatttttta ttggagataa cttatatgaa attgaatttc ctcctaaact tgtatccatt
    11281 aaatcttctc ttgctttaga taacaacatt tgattaataa ttttttgatt tactccataa
    11341 gaatccacaa tatttacttt tccatcttta tcaaaattca ttacaacgta gaatctatag
    11401 ttttctttta tatctgtaat ctgcttttgt ctttcctcat cattcatttc tgtatttttt
    11461 gactgatttt cattagaatt ttcttttgaa gcatcatttt ttgttgtatc aagtactcct
    11521 aattctttat tatcattaaa aaattcaaaa ttgctttctt tatcaactaa atagtacttt
    11581 aaattctttc ctgacgcttt aatgaaatgc tcccaatttt caaagttttc attaaaatct
    11641 gcattaatac tatcatctgt ataataagtt tctgaaccat cataagtatc tttttcttta
    11701 cttacatttt ttatatagtt catagtttct tcattgataa atagctttga tggttgtatc
    11761 atcttacctt cttgttcttc cttcatatta tgatataaaa cataacttcc tttatatata
    11821 ctatctacaa agctttcctg ttcaaatgga ttctctgctc cacctttagc acttgcttgt
    11881 atagtagaat attgactaat catacctaat gatgctgata acataagtat aaaaactata
    11941 atagtaacta tatatttatc tttaaatttt ttcaattttg tatccaacac cccacaccac
    12001 ctttaaatat ttaggatttt ttgggtcaat ttctatcttt tcccttatat tcctcacatg
    12061 taccataact gtatctgtat ttacagcatt ctcattccaa actctttcat atatttcatc
    12121 agcagaaaaa actcttcctg catttctcat aagtaaactt agtattttaa attctatagg
    12181 agttgttttt atatactttc catcaatact aacttctttt gtattttcat ttaattctaa
    12241 tccgcctatt gcaaacacac tatcgtcact ttctaccttt tgctctttat ttccaaccat
    12301 gtttagatac ttagtgtacc ttctaagttg tgaattaact ctagccaata gttctaatgg
    12361 cttaaaaggt tttgttacat aatcgtctgc tcctatatta agccccatta ttttgtcaac
    12421 ttcttctgat tttgcagata gcattattac tggaaaatta tagttttctc taagcttcat
    12481 tgtaagtgtt ataccatcca ttttaggcat cattatatca actatagcta aatgtatttc
    12541 ttggttttct ataaccttaa gaccttctaa tccattgtat cccttaaaaa cattatatcc
    12601 ttgatttaat agatatattt ctatagcatc tgctatctcc ttttcatctt ctaccaccaa
    12661 aatattatat gaattcattc taatctcctt tatattaatt tataaatttt ctttacatat
    12721 atgattatac aatatgatag aagtttttcc atttatatat atatttatat cttttgttct
    12781 ggttatttgt atttaatgtc catcttagaa acctccttct agtttaaatt taatattttt
    12841 tcattttgtt ttatatactt aatattaaaa ttaaaaactt aagatattac atatactttt
    12901 ctaaagattt tctaaagatt taaatatatt taaaattaat atacaaaaaa aagtagttct
    12961 tattgtatta attaaattaa tgatttgaaa tataaaatta atatcaataa aacctacttt
    13021 ttaatttttt aaatatttat ttttaattta ttttgttttt aatttacttc gattttaaaa
    13081 tgtccttgac ttttatttta taaatgtaca tatttcttct actggttttt tagatggact
    13141 cttcaaattg tcctctggca ctccaagagc taacattgca cctaaaaaat actctccctt
    13201 ttcaaaacca gtttctgctt ctaaaacagc ttctatctca tctgctgcat agttttgact
    13261 tgtcaaccaa caagaaccat atccaagttc aattgctgat agtgtaaagt tttcaattgc
    13321 agcacctaga ctctgcatac ctggatttct tataaataat ttatctatag tctccttagg
    13381 tgcatctata agctctaatt cataatatcc tgatggatta tatacctttg taaaaactaa
    13441 tactaaaact ggagctttta agtaaaatag tgtaaaattt ttaacaaatt ttctaaatct
    13501 attagcctta tccactgata ctttatccat ttctacaagt atttcttgtt gctttttagt
    13561 tatcacatct gctatctttt ccattaaatc acggcgtttt ataacaacaa aatgccagtt
    13621 ttgaatattc ttgcctgatg gtgctgcacc tgctgctttt atcattttta aaatatcttc
    13681 atctgataca tcttgatttt taaactttct tacactttgt cttttaaaaa tagtatcttg
    13741 taattccatt tttaaatccc cccataactt ttttattatt ttaatataat ttttaaactc
    13801 atcttatttt aatatatcat gttctaacaa atctttaaat gaatctataa aataatcata
    13861 tacatctaca ttcccaaacc catatttggc atatataaaa ggtataccta caaacttagc
    13921 actatttgca tcaccttgcg tatcaccaac atagattgga tttattaatt tatttctttc
    13981 aattataagt ttattatttt ctcctttatg caaaccagta ttaccaggac attcaaaatc
    14041 tataaaatac tgttctagtt tatgtgcttt taaaaaagat tctatatatc cagcttgaca
    14101 attacttact ataaataatt tatatttttt agatagtaat ttaagagtat cttctatatc
    14161 atcaaataat ttagctccat tttcagataa atagtcacat tctttcaaag aacattcatt
    14221 caataaattc attctttctt cttcacttaa atttggaaat aaacgatttg ctatttcctc
    14281 aaaagaaagc cccataagac ttctaaataa atcttttgtg acacttaatt ctatttcctt
    14341 atgctcactt aaaacatcct gccagacttt gcatacttct tcagttgaat cccacaatgt
    14401 tccatctaaa tcaaaaataa tactatctac cacaaaaaat cccccttatg tcaaagttta
    14461 ttatatattt ataattatat actaaaaagc agatacaatc ataaatatat tttacttgat
    14521 tgtatctgct ctacaatata aaaccaattg tcaaaatcaa attttataaa tttctaacta
    14581 caagtacaca gccttgctct actttaatct tacaagaatt actcaacata acatttgata
    14641 ttccaatagg tacagagtat ttcatataat aattatcgag agggtattct aagccagtaa
    14701 gtgttacacc cttagcatca ccatttatag ctattacaga aatagtgtcc cctgggtttc
    14761 catacaatga aatttcctca ttttcaacta tatacatctc ttctttatca gaaagtatcc
    14821 tagaatatat accatcttct tttaaataat ataatagctt tatatttgca agagtgtggt
    14881 caatccttcc ccctaaagca ccaaaaaaat caatatgatt tgctttaagt gtcttagcta
    14941 aaaaaagaca aagctctgta tcagtctcat cttttttaga tggaaatttt tcaaatttaa
    15001 cacctttatt tttatagaag tttatctttt cttcttctac tgaatctaaa tctcctaata
    15061 tataatctgg cattatttcc attttataag tgtgatttgc ccctccatct gcacatatta
    15121 tgcagtcata acattcctta attataatat ctcttgtaac ttcgtaatct tcaatttcgc
    15181 catttaaaac aatacagatt ttcattatta aatttaaact aattcttcaa gagaagcatt
    15241 ctttctaaat aaattaactg tttcttgaat gtcatcactt ccaaatattg cagaacctgc
    15301 tactattaca ttagctcctg cttgtacaac ttcggctacg tttttagggc ttataccacc
    15361 atcaacttct atatcaatgt ttaatccttg actatctata atagctttta actctttaat
    15421 cttatctaaa acacaaggaa taaatgattg tcctccaaat ccagggttaa cagacattaa
    15481 taataccata tctaaatcag atagtacatg tttaatcgta tctactggtg tagctggatt
    15541 aagtactaca ccagctttta ttccatgtga ttttatattt tgtatagttc tatgtagatg
    15601 aacacaagcc tcctggtgaa cagttattat atcacatcct gcttttacaa attcttcaat
    15661 atactggtct ggattttcta tcataagatg agcatcaaat accatattta tattttcttt
    15721 ctttaaactt ttgactacaa gtggtcctaa tgtaatgttt ggtacaaagt gtccatccat
    15781 tacatcgata tgaagatatt cacatcctgc actttctact tttcttacat cttctaataa
    15841 ttttgcaaaa tctgctgata atattgatgg tgctaattta atcatgtttc taatacctcc
    15901 tactattatt ttgtcttatt tcatgtagca gctgtatata actatcatat ctttctttag
    15961 atatttctcc atttgtaaca gcttctttta tagcacaatt aggttcattt tcatgaatac
    16021 actttgaacc aaacttacaa tcattaaatt tatcaaattc tataaaataa tctttaagtt
    16081 caacttcctc tatatcttca agtgcaaggg aactaaaacc tggtgtatct gcaaccattc
    16141 caccaaattc taattttaaa agttcagcgt gacgagtagt atgttttccc ctttttattt
    16201 tatcacttac aactcctgtt tgaagtttaa aattttcatc tatttcattt aataagcttg
    16261 attttccaac tccagatggt cctgcaaaca ctactacatt ttcttttaat tcttctttta
    16321 ctttatctat atttaactta gtaatattgc ttacaggaat gactttataa ccactaagtt
    16381 catatatatt tttaacagtt tctaatgtat cattatcatc taaatctgct tttgtaagga
    16441 ttattacagt ttctaaattt tctttttctg ccaaaactat aaatctatct aaaagagata
    16501 agtttggttt aggatttttt attgcgaata ctattaatgc tttatctaca tttgcaatag
    16561 gaggtcttat aagttctgta tctctactgt cgatttcttc taagataccc tttttttcat
    16621 cttcatcaac aatgcttatt ttgactctat caccaaccaa aggtgttatt ttctgctttc
    16681 taaatatccc tctagcctta cattcataaa taccattata agtatctaca taataaaatc
    16741 cactaatccc ctttataatt tttccttcta gcatcaaatg cctcccttta taaaatttat
    16801 ataaacacat actgtttatg agtagtatat cgtgttattc tttcctattt ttatataata
    16861 ccacattttt tttaaataac aaatcctccg tttggactaa ttacctgacc agtgataaaa
    16921 tttgatttgt ctgatgccaa gaaaatagca caatttgcta tatcttctgg agttcctagc
    16981 ctcattagag gggtttcttc cacaagagcg tctatatctt cctcattata tccagataac
    17041 atgtcagtat taatgacacc tggagctatg ctatttactc taatatttga tggtcctact
    17101 tctttagcta atgcctttgt catacctatt atacctgctt ttgttataga ataatgcact
    17161 tcacaagacg cacctgatat accccagatt gaagatatat taattatatt tccttttttt
    17221 tctgatatca tgtattttaa agcaacttgt gaacaataaa agcttccttt taaattaata
    17281 ttcatcatat tatcccaatc ttcatcagtt atatctgtaa acaacttatc ttgacttaca
    17341 ccagcattgt ttacaagtac atccaatcct ccaaattctt ttatacaata atctaccata
    17401 tcctctacat cttctctatt tgaaatatta gccttaaaaa gttttacaga aaaatttttt
    17461 tcatttaata tagtgtacaa ctctttagct tcattttctg acttattaaa atttactaat
    17521 acattgtaac cttcttttgc aaaagcttta gacatagctt taccaatccc tctagctcct
    17581 cctgtaatta aaactgtttt cttcttcata aatacttttc tccttatatt ataaccaatt
    17641 cagtatttta tacaaaacaa actgcccttt aaagaaaata ttttctataa aaggacagct
    17701 cttattttat taatatttat tctgttttac caatgtttgc gccagttcct gatgaagttc
    17761 ctccattatc tcctgagttt gagcctcctg aactatctcc attgtctcct gagtttgaac
    17821 ctcctgaact tcctccattg tctcctgagt ttgagcctcc tgaacttcct ccattatctc
    17881 ctgagtttga gcctcctgag tttccaccac tatttcctga gtttgaacct cctgagttac
    17941 ttccattatt gcctgagttt ccaccactat ttcctgaatt tgagcctcct gaatttccac
    18001 cactgtttcc tgagtttgaa ccaggttctg ttggtgtatt attctctggt gttgttggtt
    18061 ttttatctgg tactgttgga gtctgtgtat ttgatttttc tgaacctttg cttacatata
    18121 gatttacctc atcaccttcc tgagctcttg atgagccagc acctggactc tgacttaaaa
    18181 ctgtaccttc tttatatatt gagctgtatt catacttaac agttcccact tttaagttat
    18241 tttcttcaat aagttgtttt gcattactca aagtaagtcc cactacatta ggaacagatg
    18301 cctgacttga acctttactt acgacaacat ttatagtatc accctttttg acatttgtac
    18361 ttccaccttc aggtgtctgt gatattatac aattttcatc aacttcacta ttatactctt
    18421 cactttttac actcaggtat aatccttctt tttcaacagc actttgagct tcttctaatg
    18481 tcatatttac aagattagga acagtcaaag atttattgcc aaaccctcca gcaaataaaa
    18541 acttatatgc taagaatact tgagcacaca atatcagtat caaaacagcc gcaattactt
    18601 ttaatcttct tctagatttt gggctttcct gttttttctt ctttttctta gttctcttac
    18661 ttggagtact cttaggtctc tgattctttt tagctctcat tatctcttcc tcttcttcat
    18721 cttcatcatc atcttcttca taaaaatcat catagtaatc ttcatcatca tccaaatcag
    18781 ctacttcaac aggcttaacc actttttcag gagctggttt tgcaagtgtt ggatttacaa
    18841 ctttattaat ttctttttca tcaattttct tagttgcaaa atcatcatat tcttttataa
    18901 aatctaaatc aatatttttt tctatatact ctatatcttc tatcaattct tcagcagttt
    18961 gatatctatc tgcactagat ttttctgtaa gcttttttat tgtagttctt acactttgag
    19021 gaatcctaac tttttcttct gatgtaaagt ctatatcatc attaatgtgt tgaagtgcta
    19081 tagaaattgg actatctccc ctaaatggaa cttttcctat caacatttca tataaaacta
    19141 tacctagaga atacaggtca gcattattag ttacaaattt acctttagct tgctctggtg
    19201 aaaaataatg cactgaacct attatactac caatattagt cattgttgaa tttgaaacag
    19261 ctttggctat accaaaatca gccactttta ctactcttcc ttcatttgat atgagaatat
    19321 tatgaggttt tatatctcta tgtataatcc cctttttgtg cgctgcacta agtgccatag
    19381 ctatttgttt tgttatatct aatgcagtat attcatctaa tataccttca tcttgtataa
    19441 tctcttttag attcttccca tctacaaact ccattacaat gtagtgaact tttccatctt
    19501 caccaacatc ataaacattt actatatttg gatgtgaaag acttgctact gcttctgcct
    19561 ctcttttaaa ctttgttaga aattcatcat catctacaaa ttcaggtcta agtactttaa
    19621 gtgcaacagt cctgtttaat aatctgtctt tagcctcata tacaaatgcc attcctccat
    19681 caccaatctt cctgatgatt tcatatcgat ttcctaaaat tgtatctccc acaatatcac
    19741 cgtcctaatt atacttttat cactataacc gaaacattat ctttaccaga aacatcattg
    19801 gccatactta tcaattcttc acttatatta ctagttctct catattcata atctaatatt
    19861 aaatctctta tatcttcatc atcaataaac ccagttaatc catctgtcgc aagaagtatt
    19921 atatcacttt ttttgatttc tttcttaaaa atatctacaa caaccatatc atctgttcca
    19981 attgctctag ttattctatt cctctgtgga tgtcttcttg cctcttcttc tgtaatgaca
    20041 tgggccatca taagttcttc aaccacggaa tggtctatag ttattttatc aaatttttca
    20101 tctgttaaca aataacatct gctatctcca acatttgcca catataagac attattatac
    20161 actatagccg taactaatgt agttcccatt ccttcaaatt ctatatcttc catagatttt
    20221 ttgtgcacaa ttgagtttac attattataa gcctgtttta atatatcatc tatatagtct
    20281 atttttacat tatcatgttg taataagttt tcctttagaa aatcaattat attttctact
    20341 gctaatttac tggcgacttc acctttttta tgtccaccca ttccatcagc tatggcaaat
    20401 attcctattg gaccatgttc tgtatctata acttctccct cacaatagtc ttcattgttt
    20461 tttcttattt ttcctatatg ggaggcacaa ctataaacca tacgacttcc tccttacaaa
    20521 ttacttgtat tttcttctca attgcccaca agctccacta atatcagaac ccatagacat
    20581 cctaactgtg gcaggtatat tgttcttctc caaactatcc ctaaatttat aaataaatgc
    20641 tttatctggc ttttcatatt ctctttcttc aactttattt attggaatta aatttacatg
    20701 acatagcata ccttttagga gcttagctaa tgcctttgct tctttttctg agtcatttac
    20761 cccttttata agagaatatt caaatgtaac ccttctattt gtctttttta tataatatct
    20821 acatgcattc aatatttctt caatagaata agcatttgct acaggcataa tttttcttcg
    20881 ttcttcatca tatggtgaat gaagtgataa agctaaattt atagctattt ctaagtctgc
    20941 caactcatac attttaggta ctattccaca agttgaaaga gttatatgcc tataccctat
    21001 attaagacca tttttttcgt taattatttt taaaaattgt tttgtatttt caaagttatc
    21061 aagtggctca ccactcccca tcaatacaag atttgatacc ctcttaccag tatcttcttg
    21121 aattttaata acttggtcta aaatttccca tggctctaag tttctaatta atccatccat
    21181 tgtagatgca caaaaattac atcccatcct acaacctact tggttagaaa cacagacagt
    21241 aactctacta tcataatcca tcatcacagt ttctattata tttccatcat ctaataaaaa
    21301 caaatatttt tttgtattat ctaccttaga ttctaacttt aattctatat ctatatgccc
    21361 tatacatgaa acctcttcta atttatttct caaacttttt ggtatattgt tcatatcatc
    21421 aaaagttttt gcacccttat atatccaaga gaatatttga cttcctctaa agggcttttc
    21481 atctatagtt ttcataaatt ctttaagttc atcttcagta aaatttttta atactatctt
    21541 tttcttttcc attctctcac taccttattc tctttaattt tgctatgaaa aaaccatcta
    21601 taccatgaat attaggatat atctttaaat atcctttatc ttgattgtct aagtctacat
    21661 ttactgtatc aattggaaca aattcaaagt tgtgattttc ttcaacaaaa gaagtaacta
    21721 tatttatatt ctccacatct tgaattgtac atgtactata tactaaagtt ccaccaactt
    21781 tgacatattt agatgcattt tctaaaattt tcttttgtat agaagtgatg tcttttaact
    21841 cttcttcttt cttatactta atttctggtt tacgtcttat tataccaaaa ccagagcaag
    21901 gaacatctgc taaaacataa tcaaatttac ttatactatt ttcatcaatc tcagaagcat
    21961 caaatctttc tacaaacaca tttttaagcc ccaatctatt tacagtagag tttataagct
    22021 ttagtttatg ctcaaaaata tctctagcta ctacttgacc agtattgttc attaaagtag
    22081 ctaagtgagt agatttccct ccaggagcac tacatacatc caaaacaaat gagccttctt
    22141 ttggattaat tactttccct actatcatag aacttatatc ttgaattgta aataaaccag
    22201 ccttaaataa ttcatttttt tctatatttt tcattttttc gactttaata gcttcttcta
    22261 ccataggaac ttttaaacat acaataccca tttcacttaa tttttcaata agttcttctc
    22321 tacttatttt aagtgtattc gttcttatat agatactagg cttttcatta tttgcttcca
    22381 acaaatcttc agcaaattct tgtccaaatt tatctatcca gttttttata atccaagagt
    22441 tataggaata ctttatagat aagtatttta cactatcttc ctcagttatg tccattatag
    22501 tctctttagc tcttatttca tttctaagaa tagcatttat gaaaccagaa gattttttat
    22561 catatttctt agataatttt acagtttcat taactgctgc ataatctgat atactatcta
    22621 aaaataaaat ctgatatgtt cccattctta aaaagatttt aacataagtt gacatttttt
    22681 ttacctttat ttttgaaagt ttattgatta tataatctaa ataatattta ttttctatta
    22741 ctccataaat taactctgtt gcaagtcctc tatccatatc acttatttct aaatttttaa
    22801 aatgcttatt tattgcaata tttgaataat tgtcattttt ttctatatca cataatactt
    22861 taaatccaac ttctcttgca tccatataaa tttctccctt ttattaaaca taaaaaaggg
    22921 ttgtttctaa acaacccact ttacttttag tcatttcttc tttgagctat tagtaacaat
    22981 ctaagtaatt gaagtattga aaccaatgct gctgcaacat atgttaaagc tgctgctgaa
    23041 agtactcttc tactttgtct tgactcactt tcatctataa tacctaaatt cccaagttga
    23101 acgattgctc tacttgaagc attaaattct acaggcaatg ttattatttg gaataacact
    23161 gaagccgaaa acaatagtat accaattttt aaaaatggac cagccataat aaatcctaaa
    23221 aatattaaaa accaagatat acttgaagca aaattcacta ctggtactaa actacttctt
    23281 atttgaagag gtgcataacc tttagcatgt tgtatggcat gaccacattc atgtgcagct
    23341 actgcaacag aagttataga tgttccataa tatacatctt ctgacaacct tacagccttt
    23401 cttcttgggt cataatggtc acttagatgc cctctaacca tttctatctt tacatcatac
    23461 aaaccatttg aatcaagtac tcttcttgct gtttgctctc cagtatatcc tcgacgtgta
    23521 tttactctta aatacttatt tgttgttgaa ctcactttaa attgtgcata tatagtaagt
    23581 aatatagctg gaattaatac aaccattgtt gggtcaaatc cccaaaaacc gccatataca
    23641 gggtacataa ataacactct cctaaaatat atttttatta tacatcctct taatattcac
    23701 ttaacttaaa aacagtttaa ttacaaacta atatattttt tcctgttaaa aagcctatag
    23761 ctttttcaat atccatcatg tctacattag tatttacaca aggtccatta ggtcttttgt
    23821 taaatacacc taaaacagga attttcttaa tatcttgtat tccagatgtc aaatctcttt
    23881 cacatgcaac agctacaaca gctttaggtt tagtgtccat tataattttt ctagctagag
    23941 ttcctcctgt agctacaaat atttttacat tagttttttc ctttagctta attaattctc
    24001 ctatgttgca tcttccacat tctttacaat tatctatatc attagtaact tttaatttac
    24061 aactattctc ttgaatacaa tgaggtatta aaattattat atcctctgga ttaaaattat
    24121 atttattact gtatatatat tcattattta actttatata tattcttcta atatcacttt
    24181 ttgacatacc aaatgatgat gcaatgaaag atataatagg aaataatcca ctgacaatat
    24241 taaaatttat tttcattata tttgagttaa catactttcc tttaattact ctataagtaa
    24301 ctactgttga acatagtatt ataagtatta gtgatattat tataatattt atcatcaagg
    24361 agaatatttg agttaaactt attataaaaa aattaacaac tcctatcaaa attcctaata
    24421 ttaaaatcaa aatcccaaca cttgttaaat acttctttat atctgtcata cttttcacct
    24481 aacccaatac taaaccagtt tctatagtat tcccttttat atattcacca accaacattc
    24541 tctttttatt cggcatctga atttcttcaa ttaaaagtac attatctata gtacttactt
    24601 ttatcccatc tttgcttaca tcaattattg tacctacatc ttttgtgctt ttttcatcta
    24661 acactttagt tttccaaact ttcattatga catcattata ggtagtataa gcacttggcc
    24721 atggatttac tcctctaact aaattgtgta tttctctggc actttttgaa aaatctatgt
    24781 tacctaatga tttattcatt attggcgcat aggaaaattc ttcatgattt tgtacttctc
    24841 ttggagcatt tccttcttca attaatctta atgtttcttt taaagtttct gctcctatat
    24901 tcatcatttt atcatgaagt tctcctgctg ttatattttc atcaagattt acttctgtct
    24961 ttagtatcat atctccagta tctaatcctt catccatata catagttgta acaccagtct
    25021 tttcttcacc atttattatt acccaattta taggtgccgc acctctatat tttggaagta
    25081 aagaaacatg aacatttata catccaagtt taggaatctc taatatttct tttggaagta
    25141 tctgtccaaa agctacaact actattacat ctggatttaa agatttaatt ttatctataa
    25201 attctttatc tctagccttt actggttgat atacaggtat attattttca attgccagtt
    25261 cttttactgg actcatacct agttttttac ctcttccttt tggtttatct ggttgagtta
    25321 caacacccaa tatttcatac ttctcatcta ttattttttg taaacatgga actgctatat
    25381 caggagttcc cataaatact attttcatca aatcaactcc tactactttt ctattttgtc
    25441 aacaaataaa attccatcta agtggtctat ctcatgacaa aatgctctag ctaaaagttc
    25501 ttctccctct aactctattg tttcaccatt tctatttaaa gctctgacct ttacataatt
    25561 tggtcttcta acatttcccg cttctccaac aacacttaaa catccttcct catctatttg
    25621 ctccccagat gtttctatta tttcaggatt tataagttct ataagctctt ctccaatatc
    25681 tattactact actcttttta atattccaac ttgaggtgca gccagtccaa caccatctgc
    25741 atcatacata gtttctgcca tatcatctaa taattgtatt atcttttcat caattttttc
    25801 aacctttttt gatttttttc ttaatactgg ttctcctatt tgaactattt gtcttaaagc
    25861 cattataatt cctcctaaat ttataataca ctgtttggat taatatctat agatacattg
    25921 atattcttat caaatatcaa atctcgtttt gttatacata tatattttat tatacccttt
    25981 aagagattaa tttcaatatt ttcatcttta aagagtattt gccatctata attttgattt
    26041 atttttgata ttgaacatgg atttggacct aatataaatc ctaaatcatt tatacccctt
    26101 ttttctaata aataaatcaa tgatgcatgc atatttttta tattttttat cactaatctt
    26161 tcatcttcac cactaacaac tacacttaac atattattaa aaggagaata tccaaatgct
    26221 tttcttattt ttatctcatc ttcataaaaa ccctcataat catattcaat agcatgctta
    26281 atagcatagt gttcagtgtc ataagtctga agtactactt ttccctcttt atcagctctc
    26341 cctgctcttc cagagacttg agttatcaat tgaaatgtag tttcaaaact tttaaagtct
    26401 ggaaaattta atatcatgtc tgcagacaaa atacctacta aagttacatt ctcaaaatcc
    26461 agccctttac ttaacatctg tgttccaata agtacatctg cttctttatc tttaaattta
    26521 tttagtatct catctaaagc accttttttt gaagttgtat ctttatccat tctcaaagtt
    26581 tttatatctg gaaaaatata ttttaattcc tcttcaattt tttgagttcc aactccaaat
    26641 ggcttaacat aagtactttt acattcagga cactcttttg gaatttcttt ctcatatcca
    26701 caatagtggc atcttccagt attgcttttc ttatgataag ttaaagatat atcacaattt
    26761 tcacattgaa acacatatcc acactttcta caagatacaa aatttgcata acctcttctg
    26821 tttaaaaata gtattacttg attattttct tcaatcgcat aacgtatttc tttttgtaat
    26881 ttcaaactaa atatacttct atttccttta tctaactcat ctttcatgtc tacaacttca
    26941 atgtttggaa gaggcttatc atttgctcta ctttttatat ttataagttt gtattctcca
    27001 gattttgccc tataatactc ttcaattgag ggcgttgctg aacctaaaat aagagttata
    27061 ttatttttta atgccataaa tctagcaact tctatagcat taaattttgg attcttctca
    27121 gacttatatg atgactcatg acattcatca attattatta agcctaagga attaaaaggt
    27181 gcaaataaag ctgacctagc tccaattaac actcttactt tacctgcttt gacagctttg
    27241 taaacgtcat gtttttctcc ttcagatagt ttactgtgga acactccaac taaatctcca
    27301 aacctactct ttagcctatc tattgtttga ggagttaatg ctatctcagg tactaagaat
    27361 atactgtcta agccttgatt tagagcaaat tctattattt ccatgtaaac ttcagttttt
    27421 ccacttccag ttactccatg aattaagtat ggttttttat catctacaaa catattcgac
    27481 tttatctcat caactgcttc ttgttgctca ttgtttaatt ttatactttt tagtacggat
    27541 ttgtaaatac tttttggctc tctataataa tccttaaatt ctagggttat tagctttttc
    27601 ttacttagag aatttatact ttgttttgaa gcatttaata aatccaataa atcatttatc
    27661 tctacatttt cattattttt aagaaaactt aatatctctt tttgtttact tcccacattt
    27721 attttatttt gctctatata atcgtcaatc ttatcacttt ctaaactcaa agatatataa
    27781 catactttct tttcattttt gtgattttta tattcccaac ataattttat aattccattt
    27841 ttattcattg tgtacaaaga actatttaag tttggtatat attttaactt atcaacctta
    27901 attttacctt tactatcaat cactttctct actatttctc tatttttatc acttaaagtt
    27961 gatatttttt ccttcaattc aatatcattt aatccactta aactttcacc taataggact
    28021 actttataat tatttaattt gtatcccttt ggatatatca aatttataca ttctatataa
    28081 gtacacaaat atctattttt catccaataa accaactcta aatcttcttt tctaaatatc
    28141 ggattttcat ctaatatatc aataatttct tttattttta ttttttcatc taagctatct
    28201 gtaaattgaa atacaaaggc ttctgttggt ttattacctt ttccaaatgg tactaatatt
    28261 ctgtgcccaa tttttattac atcagataaa aatacaggaa tttgatatgt aaataaatta
    28321 tctgtatata tagtattact tctaactatt acctttgcat attttttcat tataaaacct
    28381 ctacttaaat ttaatatatt gttcatattt taatatactt tacaagatat taaaatctca
    28441 gaatttataa gcaatttcaa gttattaata ttaactttat tttaataatt aactattaat
    28501 tatattttat actttatgac aaaataaatg ctgaaaacta caatttaact gacatttgcg
    28561 agtttttact cgctaaaatt aaattgcagc tttcagccac tttagctgtg ataaaataca
    28621 caaatatcat atttaaaaaa ggtcttattt ctcaagcaac gctttaactt tatctaaaat
    28681 aacattggct acttcttctt ttttcatctt aggatactca gtaatatttc cctctacatc
    28741 tattattttt acaatattag tatcagctct aaaaccagca ccttctttag ttaaatcatt
    28801 tgccacaata aaatctaaat ttttcttttt tatttttcct ttggcatgtt ctataaggtc
    28861 attagtttca gctgcaaatc caattagtat cttatcttct tttatttttc ctatttcata
    28921 agcaatatca taatttctac ttagttctat tgataaatca tcattcttct ttttaatttt
    28981 tttgtcagaa taagtttttg gtttataatc tgcaactgct gcactttgaa ttactatttt
    29041 attttcactt aaatttgata ctactgcttc atacaaatct tgtgctgact ctatttgaac
    29101 aaatttttta agattttgtg gaatagaaag atttgtagga cctgatatta atgttacatc
    29161 agcacctcta gctatagctt ctcttgctat agaatatccc attttaccag aagacctatt
    29221 agttaaatat cttactgggt ctatactctc tacagttgga ccagctgtaa ctatcatact
    29281 ttgacctttt aaatcttgct ctttagtaag attagtcaaa acttcctcta ctattatatc
    29341 tggattagct agtttacctg ttccaatatc tccacatgca agccttccac tttccggttc
    29401 aataaattta tatccaaagc tctttaaagt ttgtatattt ttttgaacaa ttggattttc
    29461 atacatatta gtattcatag ctggtgctat aagaacctgt gcttttgttg ccataacagt
    29521 agttgtcaac atatcgtcag ctataccatt tgcaatcttg cctataacat ttgcacttgc
    29581 tggcactatt aagaataaat ctgctttttt tgctaatgat atatgttcaa cttcccaagt
    29641 ttttggttct tcaaacatat cttctactac ataattttga ctcagcgatt gaaatgtaag
    29701 tggtttaaca aattctgttg ctgattttgt cattataaca tggatatttg catttagttt
    29761 ttttaactta cttacaacat cacaagcctt atatgcagct ataccgccac ttatgccaat
    29821 tacaacagtt ttatctttta gcatgacctt actcctcttc tgaaatttgt tggtgttgct
    29881 cagcatgatg tctagcttct tctatctcta tttcctcttc tgtcaataat ctatatgtta
    29941 tttttccact tgcaacttct ttagttgcta cacatacagg cttttcctta gttttatttg
    30001 aaacatatgg ttcttctcca tcaattaatt ttcttgctct ctttgaaaca gtccccacta
    30061 aatagtatct gttgtctatt ttttctagta cctcattgat agatggtttt aacataatta
    30121 taattcctcc ttaaatttct ctattataat atttttatat ctgctaactt tattcttttc
    30181 agatgatatt ataccttcta tttctttagc agatgttttt acatcttcat taaatataaa
    30241 ataatcataa tccttaatca ttttaatttc ttcatatgca cagctaaatc tcttctctat
    30301 ttcagcttct gtttctgtac ctcttccaac aattctattt tttaattctt caagtgatgg
    30361 tggtagtaca aatataaata ctccctctgg gcaaacttct tttatttgtt ttgctccttg
    30421 catttcaatc tccagtaata catcttgtcc tttttctaaa cattccatta ttgccgcttt
    30481 tggagttcca taaaaattat cataaatttg agcatactcc aaaaactcac ctttttctat
    30541 catctcttca aacttttcct tacttatgaa gaaataattt actccatcaa cttctccagt
    30601 tctgggtttt cttgtagtag ctgatacaga aagttttatt gtatcatttt ctttaagtag
    30661 ttctttgcaa atagtacctt ttcctgcacc tgatggtcct gatacaacta gcaataatcc
    30721 ttttttcaca aacatttaag cattcccctt ctaatattgt attcttactc tatattttgt
    30781 atttgttctc ttattttttc caattcgctt tttacttcta ctactaaatt agttatattt
    30841 atatcagatg acttcgaacc tatagtgtta gtttctctat tcatctcttg tattaagaaa
    30901 tctatttttc tacctataga gtcgtcttta aatatagtat ttttaagttg ccctatatga
    30961 cttttaaatc taactatttc ttctgttata ctgcttttat ctgcatatat tgcaacttct
    31021 tgtgctaatc tattctcatc tattatactt gggtcatcca gtaattcact tattctcaaa
    31081 ttcaattttt ctctataatc ttctacaaca ctacttgaat atttttcaat ctcttctata
    31141 tgatttttta ataaatcaca tctcatggca atatcttcag ctaatttttt accttcttca
    31201 cttctcattt ctcttaattt gattaatgca tcttcaactg cttgattcaa cattgaccac
    31261 aataaatctt catcttcttc cttttcttct attttcacaa tatcaggaaa ctttgcaaca
    31321 ttcataacgc taatatcatc tataatatca aatttatttt ttatttcttt tagtatgtct
    31381 atatactgag atgctaatcc ctcatcaaaa tttaagttta catcttcttt gcctaataag
    31441 tcaaacttta tgtataaatc tactcttcct ctttttacat aattttttac taaatttcta
    31501 actttatcct ctaaaaatga aatttttctt ggaagacgta tgtttatatc tgaatactta
    31561 tgatttatcg ttttacactc tactaaaaag taataattat catccttgta ttctcctctt
    31621 ccaaaaccag tcatacttat agccattaat taaacctcca aaataccttc acatattttt
    31681 actgctggac ctgtcatata agagaaatct cctacatcta ttttaactgt tccaccttga
    31741 gacgttacat ttacactact ttctaccata ttaagtaaat tgcacactat tgctgatgca
    31801 gtcataccag taccacaacc taaagtatat ccacaacctc tttcccaagt ataaacatgt
    31861 atattatact tatcttctac ctttacaaaa tttacattcg ttttttcagg aaatatttta
    31921 ttattttcaa tctcttttcc atatttacaa acatcatcta catctaattc atctactata
    31981 actactgtat gaggaacccc catcaacata gaagtaactt taagctccct atctaaaacc
    32041 tttatacttt cttgcacaaa acgttcttta tttgtaagta caggaacgtc cttagctata
    32101 aaacttcctt ttcccatatt gactcttata gagtttattt ctctattagt aagatttact
    32161 tttatatctt ttataccatc taatgtatct actgtaaaac tttctttttt aactatacaa
    32221 ttatcataaa caaatttaca aaaacatcta aggccatttc cacacatagc tgctcttgaa
    32281 ccatcagaat tataatatac catttctaca tctgcaactt ttgaatcttt cacaactaac
    32341 aatccatccg ctcctacaga aaatcttcta tggcatactt tttttgctaa agcagtataa
    32401 tcagatggat ttatctggtc gaatcttcca tctattgcta taaaatcatt tcctattcca
    32461 tgtagtttcc aaaatttcat aagtatcgct ccttcgtaaa ttaacgctta aactattata
    32521 acataaaaaa attcccataa acttataatt tatgggaaaa gttttgtaat gacaactata
    32581 atttgaggat gtgagggaga gttgccatta caattagggt aattatatta tgtttttaag
    32641 ctagttcttt ctatgtttat atattaatat ttctatatga attttatgta tgttttattt
    32701 aaactgtcta tgaatttttt aattaaacat ctggtaacta gctataatcc ttctagtaga
    32761 tatctattaa catacaccta ttaattcttt aagtgttttt aaaacatagt ctaaatcttc
    32821 cttagtaata tctacatgag ttacaaatct atattcacca ttttccattt tattaatttt
    32881 tatatttctt tcaaaaaatt cttttattaa aacatcctct tttataatat cttcactcat
    32941 cttaaaaaat accatactta tatctggatt aacattgttt acttcaatgc catcaatttt
    33001 atttaattca caagctaaat attttgcatt ttcatggtct tcattcaatc ttaaagtcat
    33061 tttttcaatg gcaattatac cagctgctgc aactatacct acttgtctca ttcccccacc
    33121 cataactttt tgctttcttc tagcttttgc tataaattct ttatttccag caagtataga
    33181 acccattggt gcagctaaac cttttgacac acagaacata acactatcac aacactttgc
    33241 tatttcttta gcttctacac ccaaagttaa tgctgcgtta aataatcttg ctccatctaa
    33301 atgtactggt aagttatgtt tctcagctat ttttttaatt tctcttaaat tatcaagact
    33361 cacaactgtt ccacctgaat atgcattttc tacacatata agccctgtct gtggttcatg
    33421 tatgtcatct cttctaattg ccttttcaac aagttcagga tttagagctc cactttcgtc
    33481 ttcatctata gttctaagct gaactccagc tattacagca ggtgccccaa cctcatgagt
    33541 cacaatgtga taacctttac caataattat ttcttgccct cttaggcaat gagtaaatag
    33601 agctaattga ttcccaaaag ttccacttgg aaggaaaata gcatcttctt ttccaacttt
    33661 agctgctgct aattcttcaa gtttatttac agtagggtca tctctaaaga ttgaatctcc
    33721 aacaggagcc tttgccatag cttctctcat ttcgtcagta ggcatagtaa cagtatcact
    33781 tcttaaatcg ataaatctca ttttttcaac cttctttcat atttatatac tatagaacct
    33841 taaaattgat tttttctgta aagttcatat actttcatag cttatactct acacaataat
    33901 tatactatat ttactttata tttaatctac tttaaattat taattttaaa aataaagttt
    33961 cctctctaat aaatataaat aattatacaa tttaaatatt aatctctatt gtttaaattc
    34021 cccctagtca caaagcgtat attatgtgta taattaattt actgactcat tttggtcaaa
    34081 gaaaggggtg ctataaatgg cattagatgg attagttata cattcaatag tcgatgaatt
    34141 atcatcaaaa cttacaggtg gaaaaataga taagatacat caacctgaag atgatgaggt
    34201 aatttttaat ataagaaata ataaagaaaa ttttagattg gttttaagtg caagtgcctc
    34261 aaatcctaga gtttatctta caagtaatta tcaaaaagaa aaccctctaa aagcgcctat
    34321 gttttgtatg ttacttagaa aatacataca aggtggaaat atagtagaaa tttctcaaat
    34381 aggttttgaa agaataataa aaataagtgt tgaatcttta gatgaattaa aagaaaagac
    34441 agttaaaaat attatgattg aaattatggg aagacatagc aacataataa taactcatgg
    34501 tgaagaaaat aaaattatag attctataaa aagagtccct tttagcataa gtagagttcg
    34561 tcaagtttta cctggtcatg attattcatt gccaccagaa caaaacaaac tcaaccctct
    34621 tgatgatata tctaaggatt tgtttattaa aaacctagag gaattggaag gacctatctt
    34681 taagtctata tattctaaat ttctaggtat aagccctatt atagctaaag aaatttgcta
    34741 cagagcaggt gtaaatcaaa atgctatcat aaaggatata tctgatgaac aatttgatgc
    34801 tttacacaaa gtattttgta atttattcaa tgatataaat tctaacaaat actctccttg
    34861 cattattata gataaaaagg tggataaggt agttgatttc agttgtataa accttacatt
    34921 atttagcgat ttatcatata taaataagga tagtatgtct cgtattctgg aagatttcta
    34981 tagaactaag gatattaaag atagaattaa tcaacgttca tctgatttaa agaagagtat
    35041 atctgttaag cttgatagac tttataataa acttaaaaaa caagaagaag aattatcaga
    35101 atccgaaaat gcagacattt ataagataaa aggagaattg attacttcat atatttatat
    35161 ggtagaaaaa ggtatggaaa gtattgaagt tgctaacttc tatgatgaaa attgtaatga
    35221 tgtaacaata gaattaaata aaaacttgac accatctgaa aatgctcaaa aatactttaa
    35281 aaagtataac aaaatgaaac atgctaaagt cgagatatct catcaaattt ccttaaataa
    35341 ggaagaaata gattacttag aaaacataat tttaagtatt gaaaactgtg aaaatttagc
    35401 tgaattacaa gatataaaag aagaacttgc taaagttgga tacatcaaga ctcaaaagaa
    35461 aaacagtaaa aaggatacta tcccatctac aaaacctcat gaatttgtgt cttctgatgg
    35521 atttaaaata ttggttggta aaaacaataa gcaaaatgac tatcttacac taagacttgc
    35581 tgataatgat gacttatgga tgcatacaaa aaatattcct ggttctcatg taataataaa
    35641 gtgtgctggt aaggaagtcc ctgataatac tgtatttgaa ggagctatgc tagcagcatt
    35701 ctttagtaag tcaaaacttt cttctcaggt gcctgtagac tatactaaga gaaaaaatgt
    35761 gaaaaaacct agtggttcta aaccaggtat ggttatttat gaaactaaca gtactattta
    35821 tgttactcct gaagaagaaa cagtagccaa gcttaaggtt aaatctgaat aaattctgca
    35881 ctaaatacaa aataaatgtt agacaactac acattatcat tctaaataaa aatagtggcc
    35941 ttcttataaa gtacaataat aaggccactt tctaattagc ttacttttaa ttttctaaca
    36001 aaatactttt ctgttttaat aactatactt atattcccaa caaatatttt cagtcttctt
    36061 atatagatgt aattaaaaag ttaacactaa aagctatttt ttaatttatt gatttgtttt
    36121 agtaattcat tatattctga ttcatacaat tcttttttta gattatcttt ttctatagat
    36181 aacatagata taacagttgt aagtctattt tctaatacta gaagattttc ttttttgatt
    36241 tggttttctc tactaggagt ttttttattt ctgctaattg tatattcgtc ataattacaa
    36301 tcaaactccc ttattttagc atcttttatc tctataatat agttacaaat atgtgataca
    36361 aatactctat catgagacac tataagcata gttttatcag tatttatcag tgccttttcc
    36421 agtgcttcca tagattttat atctaagtaa tttgttggtt catcaaatac taaaaaatta
    36481 ttatcttcca atattatttt acatagtgct atttttacct tttctccacc actaagtact
    36541 tttacttttt tgtagacatc atcaccttta aacccaaata ggcttaagtt tatccttata
    36601 aaagattcat caaatgaact attaacttta gtattgtata gaacactctt ttcctcatct
    36661 aataaacttt gattttggtc aaaataacct acttttacct tgttatttat ttttatattg
    36721 tcatttttat cagccaatat ttcttttatc agagtacttt ttccacaccc attatcacct
    36781 aacaatgcta tctttttacc tctttttatc ttaaaggaaa cattatctaa caggagctta
    36841 ttttctaact ttagagtcat atcttttacc tctactagat ttttgctaat tatctccata
    36901 ccatcttgca tcttaatgtt catttcttta gaaactttag gtttattctt tacctcaagt
    36961 ttatcaattc tgctttttat agccttcaca tttgagtcca actttttctt attagtctga
    37021 ccacccattt tgtgcaacct agcttctgaa ttacccattc tttttggtgt ttttcttatg
    37081 ccatcactta aagctttttt atataaaaga gctttttcaa gccttttttt ctctttaaca
    37141 tattcttgat actcaaaatc agctcttttt acttcttcat ctttttgcat taaataatca
    37201 gtgtaattgc ctttgtaagc ttttagtttt ccatcttcta gttctaatat agttgtacat
    37261 aactcatcca gaaaacgtct atcatgagat attaataata atgctccctc atgcctttta
    37321 aacatatctt ccaaaacccc aatgcttttt ttatctaagt ttgaagttgg ctcatcagct
    37381 attatgatat ttttcttatt actcagcgca tctgctattt ttaacttaac cttttcccct
    37441 cctgataagt gttcttcaaa tttatctgga gcatttaaaa gactcttctc cttactatga
    37501 ctacatgttt caatattatt ttcactctga cttatataag aatagctttc tgtaaggtaa
    37561 gtatatcctt catcaatttc tatttctccc aatattgctt ttaataatgt tgtttttcct
    37621 tgaccatttg aaccaactag acctattttg tcattttcta atatttcaag tttatctata
    37681 tccaaaatta atttatctgc ataatatttc tttaaattct ctacttttac taacaacata
    37741 aaaaatccct ccttaacaca cttaaatata aggagagatt ctataaatag gcataacaaa
    37801 taaacgtata gactataatc tactctcctt aatttaagca ttgcaaataa agccaccaaa
    37861 ataggctttt caaaaattac aatactttaa attataaaaa atagattata ttctcattgt
    37921 cctagttacc ttgccctttc tttcatttat ttttaatcat tatatcaaca ttaattacaa
    37981 atgtctatag aaaattaact aattttagtt attcaatata caacttattc tttaaattta
    38041 ttcttcttca actttaaagt cctgattatt taaaattctg tttaatacaa ttccaacaat
    38101 tgcagctaaa ctaagaccag ttatctttac tgtacttgtt atagggattc caacagaaat
    38161 acctttatta gataaatatg ttgttccaag acctattaat aatataactg ctatgattaa
    38221 tatatttatt ttactttcta cacaagaact gtcccttata gtttttaatc ctacataagt
    38281 tatcatcgaa aatagcatta tactaattcc acccataact gaacctggta tactttgaag
    38341 aaatccacca aacttgccta cacatgcaag caatatagca aatattgctg tacgtctaag
    38401 tattgaaggg tcatagtttt tagttatagc aagtactgct gtattttctc cataagttgt
    38461 attggctggt cctcctaaac accctgcaac tatagtcgca aatccatccc caagtaatgt
    38521 tctatttaat ccgggatttt ctataaagtt tttacctact acagcaccat tagtagtcat
    38581 atctcctata tgttccataa atacagctaa tacaactgga gatataatca ctattgctcc
    38641 taaactgaac ttaggaactg taaagttagg tattgcaaat aaacttgctt ctgtaatagt
    38701 agttatatct actaatctta atatcaaaca gattatatac ccagaaaaaa ctgctattaa
    38761 tattcctaat tgttttataa atcctctacc aaacttattg gttaaaattg ctattgctaa
    38821 agttataaat gctatcacaa aattggctga agccatacta aaagctgttg gcaataaatt
    38881 taatcctata actactatca tagctcctgt aacttgtgca ggaaaatata atttaatttt
    38941 atttacacca actactttta ctataatcga cattataata taaattaatc ctgctactat
    39001 tataccacct tgagcatatg ctaaatctcc accatatgcc tctttagctg ctagtataac
    39061 tggtataaac gcaaaacttg aacctaaaaa ggcaggtact tttccttctg tacaaaaatg
    39121 aaatatcaat gtaccaacac cagcacagaa aattgcaact gacggattaa aacctgtcaa
    39181 aatgggaact aatactgtcg ctccaaacat ggctaataga tgttgtaaag acattatcgt
    39241 ttttttcata aaaacctcca aaattattat atataaataa atcctctgtt taattaatat
    39301 cttttaatta attatcattc cctagattct attttttaac ctctatatac ttattatata
    39361 atttatcatt attttacatt ttacgacatc aaaaactcaa ataaaggaga ataaaaacta
    39421 cattaagact cacatccata aaattataat atttattttg tgcaatctat gtaagcttta
    39481 tcctcctccc tgactattca atgctaagct tttatataat acttaaaaat aattattctt
    39541 tgattgtaac tgaatcttct ccatcaattt ctaataaact gacagatata atttcatgtc
    39601 tagaagtagg tacatttttt cctacataat ctgctcttat aggcaactct ctatgccctc
    39661 tgtccactag cactgctaat tgtattgatt taggtcttcc tatatctata atagcatcta
    39721 gtgaagctct cacagttctt ccagtgtata aaacatcatc aactaaaact actgtcttat
    39781 catttacatt aaaatctaaa taagttcctt taactacagg ttcaacatga attttttcca
    39841 aatcatctct atacaaagtt atatcaatat ctcctgtatc tacttttgtt ccttctattt
    39901 gttctatttt cttagaaatt ctatttgcaa ttggcacacc tctagtcttt attccaacta
    39961 aaactagatt ttctacacct ttatttcttt caattatttc atgacttatt ctagtaattg
    40021 ctcttgctat tgccttttca tccattaatt gagctttttc taccatatca taattattgt
    40081 caaagatata atatatctat ttgacaaatc tactcctttc ttttaatttt ttataaattt
    40141 atttttattt aaaatttcaa tttaaacaac aaaaaaactt ctcatcacaa gacaagaagc
    40201 tcttatataa aaaatataca gtgcaattta gcaattgtct tacgatatct ctgtatcgtt
    40261 taaagataat tttttaatct atcaaatcaa attatatatt tatatttcaa atttttcaag
    40321 cttttttaaa caaaatactt taatataaga tattttattt aaatttcact aaatttattt
    40381 acttatataa aaacaaaaaa tgatttataa gtattataca aatagttatt ttatcttttt
    40441 aataacttcc ttaaaatact caggtaattc tgaattaaat tcgatatatt ccttagtagt
    40501 aggatgtata aatcccaact tcttagcatg caaagtttgt ccttttaatt taaatttagt
    40561 ttcttttgga ccatatacat aatcacctaa aagtgggtga tttatagaca aagcatgaac
    40621 tcttatttga tgagttctac cagtttcaag cctagctctc atatgtgtaa acttatcaaa
    40681 tctttctaca acttcaaagt gagtgattgc attttttcca tcttttacta cagccatttt
    40741 taatctatcc ttaggatttc tccctatagg tttatcaaca gttattttat cttctttaac
    40801 cacaccatag catataaatt catactccct agttatagta tgttccttta actgttctgc
    40861 tagaaaatta tgtgcataat tgttcttagc tatcataaga agaccagaag tatctttgtc
    40921 tattctatga actataccag gtctaataac gccatttatt gatgacaact tatctttaca
    40981 atgatataaa atcccgttta ctaacgtatt ttcatagttt ccaggagcag gatggacaac
    41041 catatcctga ggtttattta ttattaagac atcatcatct tcatatacaa tatctattgg
    41101 aatatcttga ggtaaaactt ctaatacttt aggttctggt atctctataa caatattatc
    41161 ttcctcttta accagatatc tagctttttc tactttatta ttaaccttta cttttccatc
    41221 ttttataatt ttttgtatat aacttcttga catatcacct aattgttctg ctagataaat
    41281 atcaagtcta acttcttctt cctcttcaag tactaaaaac tcttttactt catccattat
    41341 atcacctact tttactttca aaaaataaaa catatataca taaaaacaca gttcctacaa
    41401 ctacaaatac atcagctata ttgaatacat attcccatat aattctaaag tcgaagtaat
    41461 ctactacaaa acctaatcgt actctatcaa ttagatttcc caatgcacca gatataatta
    41521 atataattcc caaccttcca aatatatgta ctttccttgt attaagatag tatagtccaa
    41581 atactgttgc aagtaatgca acaattataa atatccattg attattttgt aataatccaa
    41641 atgctgcacc tctattttcg acataagtta aatgaaatac attatttatt attggtattg
    41701 ttgatacatc caccaaatta ttcaataccc atattttaga cagttggtct aaacctatga
    41761 gtagaattat tattaatata tatagcaatg atttttcccc ctataaatta tttaaaaacc
    41821 aattttattt gaagcacact attattatta tacaaaaaaa atgctctaaa gagcaacttt
    41881 attaaaattg tattttctta ataaagctag aacaaaattt aaaatagtac tacttttttt
    41941 atattgtaga tttctacata tttaaaatca tatcattatg tataaaaaat ttaaaaatac
    42001 catactaatc aatatttttt gtgtatttta tagattaaag attaatgatg tctgtaattt
    42061 aaacttacaa tcaccactaa tcttcaaaca aatattttta cttttgcact tgcttatttt
    42121 tatctaaatg ttgtataagt tcagatactg ttacgaattt atatcctctc tcttttaatt
    42181 taggaattac tacttctaaa gcttgtatag tttgtgattt atcatttctt ttagtgttat
    42241 aatcatgtaa aagtattata ttaccatttt ggacctttgt aaggatagta tttataattg
    42301 catctactcc agggttagac caatctctag ggtctaagtt tgaccaaaga attatattca
    42361 tgcctttaga aactataata tcacagacac ttttactcat ggctctatat ggaggtctaa
    42421 atatctttgg ttctactcct attacacttt ttataatatt ttgcgtatct gttatttctt
    42481 tttcaacact actataacta ttttttgcaa catttatatg tgtaaatgta tgattcccta
    42541 tttcatgccc ctcatcatat tgtcttttta ataattcttt gttatattct actttttctc
    42601 caactacaaa aaaagttgct tttacatcat ttttctttaa tatatcaagt acttgtggtg
    42661 taaaatcctc atctggacca tcatcaaaag ttaatgctaa aattttttca tcctcatttc
    42721 ctttttttat tattacatct tcataatcgt cttttatatt gtcatttaaa ctatatgctt
    42781 cttttgaact atcttgatta tgtgtattat gattatatgt atatacacct actatagata
    42841 ataaacatat acatataccc catattattt tcttcatgtc attacctctt ttctaattca
    42901 ttctagcaat atattttata atgtcagttt tttaatctct caatattcca tttttattta
    42961 aatcagttaa atttgttagg tcttctgaat aaaagttaga atcttctaca tcagggtttt
    43021 gcattgcatc acctaataaa ttattttctt tctttgcaca atcattacat aaacttgtat
    43081 ctggcaatat atccaatctt tctaaatcta tttgattatg acataaatca caaatcccat
    43141 atgttccatt ctctatttta gataatgcta agtctatttg atacaatctt ccctcttcat
    43201 gatttattag actgttttgc atattttgca tatataaatc tgttcccatg tctcctatat
    43261 gattatcata actagataat tctcctgatg aatacttttc acttgtatgt ttagttgtat
    43321 ctccaaataa agtattatct ttcatatcct ctactaaatt agttaaattt tctctttctt
    43381 tcaataattt atctttatat tttttagtat ccatttttaa cacctctaat tttaatattt
    43441 acatcataaa aatagtatgt ggaaaagtaa attaaacata caataaaaat aagatttgat
    43501 tcgaaaaatg gaatgtctca aaatgaaatt tttagttcat ttaaccctct tttttatatg
    43561 aaatcaaata tatttttatc ctttcagcaa taaaaaagag acttatctct attttgagat
    43621 agtctcttct ttgaacataa tttaaataca attttttaat ttttatagtt attattttac
    43681 taacttgcca atttatgatt attttgttct ttaatatgtt ttgctttttt aaagaataaa
    43741 agaccaagaa ttattgctac aataacacct attccattag atatattaac tggtagtcta
    43801 aatccttctt ttgcttgcat tatataacta aatgttacta cagtcataaa aagtgctggt
    43861 aatgtagtta tataatggtt tttatttgct ttgactaaat atgcagaacc agtccacaac
    43921 attatcattg caagtgtttg atttgcccaa gagaaatatc tccaaattat attaaaatct
    43981 ataaatgtaa gagcaacacc aactgcgaat aatggtaaag ctattaaaaa tctatttttg
    44041 actgcatctt gattataatt cattgcatct gctattgtca gcctagcact tctaaatgct
    44101 gtatctccag aagttatagg acatgccaca actccaagta tagctaaagc tcctccaata
    44161 ggccccatta atccaacaga tactgtattt actactgttg ctgctccacc atcattaaat
    44221 atcacattaa gttgaggtat gccatggaaa tatgacatgg ctgctgctgc ccaaactaaa
    44281 gctattatac cttctgcaac cattgaccca taaaatactg gtcttgcctc actctctttc
    44341 tcaacacatc tcgccataag tggtgattga gttgcatgaa aacctgatat tgctccacat
    44401 gctattgtta taaataagta tgggaataaa ggtgttccat ttggattcat attttgtaaa
    44461 tttatatttg gtatgttata cccctttatt attaatcctc cagctacacc tactgccata
    44521 attaataatg ctgctccaaa taaaggatat attttaccaa tcaatttatc tattggtaaa
    44581 acagtagcca taatataata aagtattatc actccaaccc atatttgtgc gtttacacct
    44641 gtcaatgtct ttaataatcc tgctggacct gttacaaata caactccaac caatactaaa
    44701 agaataacac taaatattct cattgctatt tttgcaccat ttcctagata aaggcctacc
    44761 aactctgatg cagatgtacc tttgtttttc attgatagat atcctgaagc aaaatcgtgt
    44821 actccaccag caaagattgt tccaaacaca atccataaaa atgctgatgg tccaaataat
    44881 gctccttgta tagctccaaa tatcggacct aaccctgcta tatttagaaa ctgaattaaa
    44941 aagattctct tccatttcat tggcacataa tctactccat ccctacaagc tatagctgga
    45001 gtttgatttt tgtcattggt tccaaaaacc ttttctacaa aagtaccata gacaaaataa
    45061 cctactataa gtactactat tgaacctaaa aagcttacca taaattcatc cccttagttt
    45121 ttatataatt gctttataaa tttttatgag tttaagaaaa ctatttccag ttcaaatagt
    45181 aatacttttt tatgctaata taaatattaa ttacattaag tgcatattta agtacatgaa
    45241 atgactttat atatgcatta acagcctaaa ttcttttact ttgcttctac ttacagttac
    45301 ttcataatca ctatttttta attttaatat gtatgtatta ttaaaccatg gtataatttc
    45361 ttttactttg gttaagttta cgatataaga cctatgtgtt ctaaaaaatg ttttatcatt
    45421 tataagattt tcgacttctg atatgccttc ttttaatata aacttttctt tctcagtata
    45481 tataaatgtc tgcctttcat tagcttcaca gtaatatata tcatcaatat ctattacaac
    45541 taatttatca cctttccaaa ggctaatttt atgtgttatt tcttctatct cctgatttac
    45601 tgcttccttc ttatatttgt ataaattact atttacatta aatagttcaa tatcactcat
    45661 ctcacttttt tctaatttat tcagcataga aataattctc tcttcagaat atggtttcaa
    45721 tatataatca aatacatcca aagaaaaagc atctactgca taactttcat atgctgttat
    45781 aaaaacaatt ttaggtcttg atttaaactg atttaatgtt tttgcaagta gcattccatc
    45841 aagatgaggt atatttatat ctaaaaaaat tacatctatt ttattttcct gaataaagtc
    45901 taacacctct ataccattgg taaattcact tactacctcg attccacttt tattttcaat
    45961 aaaatatctc aattcctttc ttgctggaaa ttcatcttcc actattatag ctctcatact
    46021 tctactcctt tacataaaat actatcaaag tcccattatc tattcttctt atattaagcc
    46081 ctcttccata catcagttta agccttaaat gtacattata aagcccaatc ttattttctg
    46141 gcatattatc ttgatataca ttatttataa tttcctcact tatacctata ccattatcct
    46201 ctattgatat tctcacagta ttttctaatg aatacggtaa cttttttatg gatattttca
    46261 ctacaccagc tctaccactt tctaaaatac catgtattat tgcattttcc actaatggtt
    46321 gtataatcaa acttggaatc tttatattta catcttctat atcataagat acagttagta
    46381 attctccaaa cctagctttt tctatctcta caaaagattt aacttgttca atctctttat
    46441 ttatatcaat taagttatca ctatattcaa gattatatct tagataagtt gataaattta
    46501 ttattaactc tctagcctta tctggactaa atcttataaa tgaagctatt gtgtttaggg
    46561 aattaaacaa aaaatgtgga ttaatttgtc tttgaagtgc ttttatttct gctttatttc
    46621 tcgcttctat atctttttta tctttctcaa tcccctctac tatagatata acaaatccta
    46681 ttccaatctg cccaactatt aaaggtaaat atattccacc gattatttga attgctaaag
    46741 aatgtggata tgatattaag tatataagta atatagtaaa actttcagaa atcatgccta
    46801 ccaatattcc atacattact ctatgttgtt ttggtatcct cttatataga aatcctgata
    46861 atacacctgc tagtatactt gataaaagac atggaacaga tgttatcccc ccaatatcca
    46921 taaaatatct atgaatccct gaaaaaactc ctgctgtaat acctaccatc ggaccaaata
    46981 aaatgccacc tgatactata gatattattc tagtgtttac tattgacccc atataattta
    47041 taccactata agttccaaat atggctaaaa aagtaaaaac taatgctata cagaccaagt
    47101 cattaaaact atattcttcc ttttgaaata tctgcttaaa tatttttaac tttgttatta
    47161 gaagaaacaa tactacaata aggcttgtct tttctattaa tgcgattaaa attgatgttt
    47221 tcataatctc cctttaaaaa ataaattttt acacactcta aatttattat ggttgttata
    47281 aattacatat ttgaagtaat cctatcacaa aactttccac atttaatctt ttaattaaat
    47341 aagaaatagt tgtacttcta ttaacaacta atactagcta gcataagctt gtaataactt
    47401 gtagttatta ttaactttac aattctagta tttaatttta tactttagaa atctcttttc
    47461 ttagaaataa tactttcgaa tatcatataa tacaatatta tgtagattct tactcataca
    47521 acatatttct ttaaaaattt tatactattc tttgttcaaa tatctcattt gatttaattg
    47581 tgttaaacac acattatcat ctctctgtta tat
//
LOCUS       DAMKMI010000015        44347 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMKMI010000015 DAMKMI010000000
VERSION     DAMKMI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 44347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.015"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            512..1351
                     /locus_tag="PC429_000809"
     CDS             512..1351
                     /locus_tag="PC429_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF3846327.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1348..2529
                     /locus_tag="PC429_000810"
     CDS             1348..2529
                     /locus_tag="PC429_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846328.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            2833..3735
                     /gene="ptb"
                     /locus_tag="PC429_000811"
     CDS             2833..3735
                     /gene="ptb"
                     /locus_tag="PC429_000811"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF3846329.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            3757..4836
                     /gene="buk"
                     /locus_tag="PC429_000812"
     CDS             3757..4836
                     /gene="buk"
                     /locus_tag="PC429_000812"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF3846330.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            4826..5503
                     /locus_tag="PC429_000813"
     CDS             4826..5503
                     /locus_tag="PC429_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3846331.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            5546..5761
                     /locus_tag="PC429_000814"
     CDS             5546..5761
                     /locus_tag="PC429_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3846332.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            5790..6869
                     /locus_tag="PC429_000815"
     CDS             5790..6869
                     /locus_tag="PC429_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF3846333.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            6869..7621
                     /locus_tag="PC429_000816"
     CDS             6869..7621
                     /locus_tag="PC429_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF3846334.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            7622..8179
                     /locus_tag="PC429_000817"
     CDS             7622..8179
                     /locus_tag="PC429_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF3846335.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8349..9695
                     /locus_tag="PC429_000818"
     CDS             8349..9695
                     /locus_tag="PC429_000818"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF3846336.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            9962..11794
                     /gene="glmS"
                     /locus_tag="PC429_000819"
     CDS             9962..11794
                     /gene="glmS"
                     /locus_tag="PC429_000819"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF3846337.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            12245..13540
                     /locus_tag="PC429_000820"
     CDS             12245..13540
                     /locus_tag="PC429_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF3846338.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNRQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            13997..14734
                     /locus_tag="PC429_000821"
     CDS             13997..14734
                     /locus_tag="PC429_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF3846339.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTKYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            14755..16008
                     /gene="murA"
                     /locus_tag="PC429_000822"
     CDS             14755..16008
                     /gene="murA"
                     /locus_tag="PC429_000822"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF3846340.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            16095..17159
                     /gene="spoIID"
                     /locus_tag="PC429_000823"
     CDS             16095..17159
                     /gene="spoIID"
                     /locus_tag="PC429_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF3846341.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            17225..17893
                     /locus_tag="PC429_000824"
     CDS             17225..17893
                     /locus_tag="PC429_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF3846342.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            17991..18251
                     /gene="spoIIID"
                     /locus_tag="PC429_000825"
     CDS             17991..18251
                     /gene="spoIIID"
                     /locus_tag="PC429_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF3846343.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            18448..19467
                     /locus_tag="PC429_000826"
     CDS             18448..19467
                     /locus_tag="PC429_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF3846344.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            19483..19911
                     /gene="fabZ"
                     /locus_tag="PC429_000827"
     CDS             19483..19911
                     /gene="fabZ"
                     /locus_tag="PC429_000827"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF3846345.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(20022..20531)
                     /gene="yyaC"
                     /locus_tag="PC429_000828"
     CDS             complement(20022..20531)
                     /gene="yyaC"
                     /locus_tag="PC429_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF3846346.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      20720..20823
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            20959..22152
                     /locus_tag="PC429_000829"
     CDS             20959..22152
                     /locus_tag="PC429_000829"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF3846347.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            22467..23564
                     /locus_tag="PC429_000830"
     CDS             22467..23564
                     /locus_tag="PC429_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846348.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMTIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            23828..26047
                     /locus_tag="PC429_000831"
     CDS             23828..26047
                     /locus_tag="PC429_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF3846349.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHVEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            26050..26832
                     /locus_tag="PC429_000832"
     CDS             26050..26832
                     /locus_tag="PC429_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF3846350.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKDNVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            27164..29077
                     /locus_tag="PC429_000833"
     CDS             27164..29077
                     /locus_tag="PC429_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3846351.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIGGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMFTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSSNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIRIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            29218..29526
                     /locus_tag="PC429_000834"
     CDS             29218..29526
                     /locus_tag="PC429_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3846352.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            29550..29876
                     /locus_tag="PC429_000835"
     CDS             29550..29876
                     /locus_tag="PC429_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846353.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            29915..31249
                     /gene="celB"
                     /locus_tag="PC429_000836"
     CDS             29915..31249
                     /gene="celB"
                     /locus_tag="PC429_000836"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF3846354.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQVVLLILGIFMYIPFIKMLDKQYLREENATVEEEDDDISFDDLVL"
     gene            31260..31778
                     /locus_tag="PC429_000837"
     CDS             31260..31778
                     /locus_tag="PC429_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF3846355.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGIMMEQFLNE
                     SKVIATLFCGDEFFVNNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            31874..32833
                     /locus_tag="PC429_000838"
     CDS             31874..32833
                     /locus_tag="PC429_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF3846356.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            32882..33934
                     /locus_tag="PC429_000839"
     CDS             32882..33934
                     /locus_tag="PC429_000839"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF3846357.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            33937..34611
                     /locus_tag="PC429_000840"
     CDS             33937..34611
                     /locus_tag="PC429_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF3846358.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            35164..35715
                     /gene="raiA"
                     /locus_tag="PC429_000841"
     CDS             35164..35715
                     /gene="raiA"
                     /locus_tag="PC429_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF3846359.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            35847..38522
                     /gene="secA"
                     /locus_tag="PC429_000842"
     CDS             35847..38522
                     /gene="secA"
                     /locus_tag="PC429_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF3846360.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            38537..39644
                     /gene="prfB"
                     /locus_tag="PC429_000843"
     CDS             join(38537..38611,38613..39644)
                     /gene="prfB"
                     /locus_tag="PC429_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF3846361.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            39788..41929
                     /locus_tag="PC429_000844"
     CDS             39788..41929
                     /locus_tag="PC429_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF3846362.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLEKLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMPPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DNEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKTIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNICEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            42077..43243
                     /locus_tag="PC429_000845"
     CDS             42077..43243
                     /locus_tag="PC429_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3846363.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      43303..43420
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            43544..44149
                     /locus_tag="PC429_000846"
     CDS             43544..44149
                     /locus_tag="PC429_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3846364.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPFVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVILSLVTLPLYKKMGNVIKK"
BASE COUNT        17965 a         4750 c         8445 g        13187 t
ORIGIN      
        1 ttcactgtag gagattatat ctagttatac ataataagtt ttaatgatag tttatagtaa
       61 gatattaaaa taaatttatc taatctagat atgaaataag ttattacttt aatataaaaa
      121 tgaatctaat ggaataatat tgatgataag taatatttag attttgtatt tgtttatagt
      181 taataagtgg attatagaat ttataataat taatatatat taaacaaaga tatattaaga
      241 tattttagaa cataaaactg aattaaggcg cgagtactat tttgtattcg cgccttgatg
      301 gtttgagtta tttgagtaaa aactaacttt aataagaaat atgtatttat taagttgata
      361 tttttaatgt gtttagagat tatttaacaa tctccaaata taaaactaga attatttaca
      421 taaaagttaa atagtttgta tagagttaga tttatatagt ataatactta gtgtaaataa
      481 ttaagaagtt atcaaacagg aggataaaac tgtgttagat ataaattctt ttttaaatgg
      541 attttttaat ataatattaa gaatgagtat atatgattta attgacatat ctatagtagc
      601 gtatattttt tacaaaatat ttatgtttat aaaagataca agagcagaac aagtgtttaa
      661 aggtataata tttcttttat tagcaactca acttagtaac acctttaaat tgcataccgt
      721 ttattggata tctcttaaag ctttagatta tggagttata gctgctctta taatttttca
      781 accagaattt agagcaggac ttgaacatat aggaagagca aagtttaatt tatttggaaa
      841 gaatgtaaat acttctgaag aaactctcaa tagaaatata gaagaaattg tagaagcttt
      901 atattcactt tctagacaaa aaataggagc tcttatcata atggagagag aaactagaat
      961 aagtgatata ataaatacag gaactattat tgatgctgaa atttctaggc aactattaat
     1021 aaatatattt ataccaaata caccattaca cgatggagct gttgtaatta gagattctaa
     1081 ggtaaaagca gctgcatgtt ttttgccatt gacagaaagt aaggatttaa gtaaagattt
     1141 gggcactaga catagagcag gaataggggt aagtgaggtg tctgactgta taactttgat
     1201 tgtatcagag gaaacaggtg gcgtatcaat tgctaaagca ggtaagttat atagagatat
     1261 ttctagagaa agaatgatga atatattacg tagtaattta aaaacaaata cagaaacaag
     1321 aagttttttc aaaggtggta tatttaaatg ataaatagac taaaaaataa taccaaaata
     1381 aaaataatat catttttaag tgcaattgca ttatggatgt atgttatggc tgtagttgac
     1441 cctgaagaaa ctaaagggtt tgaagatata cctgtatcta taagtaatat gagtgattta
     1501 aaggataaaa atcttgttat atatccagac gaaaaattaa cagcagatgt atatataaaa
     1561 ggtagtttat caaaggtaca aagtgttaaa aaagaagata tacatgtata tggaaccatt
     1621 gaagacccaa tacaaggaca gaaggaagta tatttaaaag caaatattcc tcaaggtgtt
     1681 actctagaat ttaagcatga tgttttagta gtaaatcttg aaaaaaacat aaaagaaaaa
     1741 agagatataa aggtagaagt aagtggaaat tcaaaaagaa atataaataa aattgaactt
     1801 agtaaggaat ttgtcgatgt atcaggtcca agaactctag ttaaagaagt caaaagtgta
     1861 agagctaatc tagatgttgg taatgagaca gatgattttt ctaaaaaatt gcaattaata
     1921 ccattaaata gtgatggaac taaggttgaa ggagtaacat taagttcatc ctttgtgact
     1981 gcaaagatag agctattaaa ggaaaaaatt gtcccaataa gagtaagtct aaatgataat
     2041 aaagataatg atgtactgaa aaactataaa ttaagtaagg aacaagttac aataaaagga
     2101 aagaaagaag atatagacaa aataagttat attaagacaa aacctattga cttagctgat
     2161 ttggctaatg gtgaagaaaa agaagttagc ttagaaattc cagagggtat aactcttgag
     2221 gatagctcta taacaattaa attaaatgat acaaaacaat tgagtgcaga atttttatat
     2281 aatgcgggag atattgaatt aagaaatatc ccaacaaata tggataatcc aaatgtaatt
     2341 tctgatgtag atataaaagt tgttgtagag tcaacggagg atttgagtaa gttaaataaa
     2401 aaagatattt cattatatat tgatttatca caaggtcctg aggaagatag taaatataaa
     2461 ataaaatatg aaacaacata tcaatttaaa aaaataaata tagaacctaa tattgttgaa
     2521 gtggaatgaa caaatatttc gaatatttag ttgttacaag atgcgacaaa aaaacctata
     2581 aaattaagtt tgttaatttt atgtttttgt tgcatcttgt ttattttagt tatattataa
     2641 aataaggaca atgagatatt tgttaaatct aaaaagcgct agatatatga aaaaagactg
     2701 ataaatagat ttaaataaat attttgaatt aaatatttca attatctcta ggaaaacgtt
     2761 gctgaacgag ttataattta attgagatat aaaaagttct atttcaaaaa attcaagatg
     2821 aggtggaaat gcatgagaag ttttgaagaa gtaattaagt ttgcaaaaga aagaggacct
     2881 aaaactatat cagtagcatg ttgccaagat aaagaagttt taatggcagt tgaaatggct
     2941 agaaaagaaa aaatagcaaa tgccatttta gtaggagata tagaaaagac taaagaaatt
     3001 gcaaaaagca tagacatgga tatcgaaaat tatgaactga tagatataaa agatttagca
     3061 gaagcatctc taaaatctgt tgaattagtt tcacaaggaa aagccgacat ggtaatgaaa
     3121 ggcttagtag acacatcaat aatactaaaa gcagttttaa ataaagaagt aggtcttaga
     3181 actggaaatg tattaagtca cgtagcagta tttgatgtag agggatatga tagattattt
     3241 ttcgtaactg acgcagctat gaacttagct cctgatacaa atactaaaaa gcaaatcata
     3301 gaaaatgctt gcacagtagc acattcatta gatataagtg aaccaaaagt tgctgcaata
     3361 tgcgcaaaag aaaaagtaaa tccaaaaatg aaagatacag ttgaagctaa agaactagaa
     3421 gaaatgtatg aaagaggaga aatcaaaggt tgtatggttg gtggaccttt tgcaattgat
     3481 aatgcagtat ctttagaagc agctaaacat aaaggtataa atcatcctgt agcaggaaga
     3541 gctgatatat tattagcccc agatattgaa ggtggtaaca tattatataa agctttggta
     3601 ttcttctcaa aatcaaaaaa tgcaggagtt atagttgggg ctaaagcacc aataatatta
     3661 acttctagag cagacagtga agaaactaaa ctaaactcaa tagctttagg cgttttaatg
     3721 gcagcaaagg cataatttta agggggaaat aggcaaatga gcaaaatatt taaaatctta
     3781 acaataaatc ctggttcgac atcaactaaa atagctgtat ttgataatga ggatttagta
     3841 tttgaaaaaa ctttaagaca ttcttcagaa gaaataggaa aatatgagaa ggtgtctgac
     3901 caatttgaat ttcgtaaaca agtaatagaa gaagctctaa aagaaggtgg agtaaaaaca
     3961 tctgaattag atgctgtagt aggtagagga ggacttctta aacctataaa aggtggtact
     4021 tattcagtaa gtgctgctat gattgaagat ttaaaagtgg gagttttagg agaacacgct
     4081 tcaaacctag gtggaataat agcaaaacaa ataggtgaag aagtaaatgt tccttcatac
     4141 atagtagacc ctgttgttgt agatgaatta gaagatgttg ctagaatttc tggtatgcct
     4201 gaaataagta gagcaagtgt agtacatgct ttaaatcaaa aggcaatagc aagaagatat
     4261 gctagagaaa taaacaagaa atatgaagat ataaatctta tagttgcaca catgggtgga
     4321 ggagtttctg ttggagctca taaaaatggt aaaatagtag atgttgcaaa cgcattagat
     4381 ggagaaggac ctttctctcc agaaagaagt ggtggactac cagtaggtgc attagtaaaa
     4441 atgtgcttta gtggaaaata tactcaagat gaaattaaaa agaaaataaa aggtaatggc
     4501 ggattagttg catacttaaa cactaatgat gctagagaag ttgaagaaag aattgaagct
     4561 ggtgatgaaa aagctaaatt agtatatgaa gctatggcat atcaaatctc taaagaaata
     4621 ggagctagtg ctgcagttct taagggagat gtaaaagcaa tattattaac tggtggaatc
     4681 gcatattcaa aaatgtttac agaaatgatt gcagatagag ttaaatttat agcagatgta
     4741 aaagtttatc caggtgaaga tgaaatgatt gcattagctc aaggtggact tagagtttta
     4801 actggtgaag aagaggctca agtttatgat aactaatgat aaaagaataa gaatcataac
     4861 tggtcactat ggaagtggta aaagtgaatt tgcgatgaac tatgttgtta agttaagaga
     4921 tatggtttct ggcaaagttg ctatagccga tttagatgtg gtaaatgtat actttagaac
     4981 tagagaaaag aaagagttaa tgaaatcctt aggtatacaa ccaatagata gctcaattaa
     5041 tgctccaaca ttagatttac cagcagtatc agcagaggta atgtctccta tggtagacca
     5101 ttcatacaat tctgttatag atttaggtgg agataatgta ggtgctaggg ttataggaag
     5161 atttagtcat ttgttaaaag aaggcgatta tgatatgctt tttgtaataa atgcaaatag
     5221 ggagaaaact caaacttctg aagaagttat ccaatatata aaagaaatag aaaaatcttc
     5281 taaattaaag gtaacaggtc taattaataa tactcatctt attaggttta ctactattga
     5341 tgatgtttta agaggtcaga aagttgcaaa agaagtttct gaaaaatgta atataccgat
     5401 aagatatgtt gcatgtttag aaaatcttgt tgaacagtta ccaaaagatt tagaaggaga
     5461 aatttttcct ataaaattat atatgagaga agactggatg tagtttgagc tcaaatatat
     5521 agaaagttta aggaggctta ggtaaatggc taaaggaaaa gtatctttta atcaagaacg
     5581 ttgtaaaggt tgtggattgt gtgttgaagc atgtccagta aaaatcatac aattagactc
     5641 aaatgtaata aacaaaaaag ggtataaccc agcaactgtt tttgaaatgg aaaaatgcat
     5701 aggatgtgca agttgtgcaa ctatgtgtcc agatgtagtt ataacagtag aaagagatta
     5761 atttaattaa tatttaaggg ggaattttaa tggctaagat acttatgaaa ggtaacgaag
     5821 catttggaaa agcagctata gaagctggtt gcaaatattt cttcggttac ccaataactc
     5881 cacaaagtga attaccagaa tatttatcaa gagagttacc taaaataggt ggagcgttcg
     5941 ttcaagctga atcagaagtt tctgcgataa acatggttta tggtggagca ggtgcaggag
     6001 caagagttat gacttcttca tcttcaccag gagttgcttt aaagcaagaa ggtataacat
     6061 atgcagtagg agcagaagtt ccttgtgttg tacttaatgt aatgagaggt ggtccaggac
     6121 ttggaagtat acaaccttca caagctgact actttatgtc tacaagaggt ggaggaaatg
     6181 gagactacag aactccagta ttcgcaccag ctacagttca agaagctgtt gatatgataa
     6241 tggaagcttt tgacgttgca gattattaca gatcaccagt tatggtagtt gcagatggta
     6301 tgataggtca aatgatggag ccagttgaat ttagagctcc tgagaagaaa agagaattgc
     6361 ctcctaaaga ttgggcaact gttggaacta aaggaaaaag aaaacctaat gtaataaact
     6421 ctctatactt agagccagaa gtactagaag accattgttg gcatttacaa gaaaaatttg
     6481 atgcaatgga aaagaatgaa gtacaatacg aaatgtacaa aacagaagat gctgaatttg
     6541 tatttgcagc ttatggaact acttcaagag tagtaaaaag cgcaattgat atattaagag
     6601 aagaaggaat aaaagcaggt cttataagac ctaaagtatt atggccattc ccattcgaag
     6661 cattcaacca aattcctaat gctagaaaca tattgactgt tgaaatgagt atgggacaaa
     6721 tggtggaaga tgttaaaatg gcagtagaag gtaaattacc agtttacttc catggaagac
     6781 caggaggaat gactccaaca ccagcagaaa tagttgaaaa agctaaaaaa attattgccg
     6841 gagaattggt tgcaggaggt gctagataat ggctgttgta tttaagaaaa ctgaaggatt
     6901 acaagatact caaactcact actgtccagg atgtacacat ggtataatcc atagattagt
     6961 tggtgaagta ttagaagaac taggagtatt aggtgatgct gtaggtgttg ttccagttgg
     7021 atgttcagtt ttaggatata aatattttaa ctgtgacact caagaagctg ctcatggtag
     7081 agcgccagct gctgctacag gaataaaaag agttcatcct gaaaatacag tattcactta
     7141 tcaaggtgat ggagacttag cttcaattgg tactgctgaa atagttcatg cagctgctag
     7201 aggagaaaaa ataactacta tatttgtaaa caatacaact tatggtatga ctggtggaca
     7261 aatggctcca actacattag ttggacaaag agctactact gctcaatcag gaagaaatgc
     7321 tgaaactcaa ggttatccaa taagagtaag tgaaatgtta gctacattaa caggagctgt
     7381 atttgtagaa agagtagcag ttgatactcc agctcatgtt agacaagcta aaaaagctat
     7441 aaagaaagca ttccaagttc aacaagcagg attaggattt ggaatagtag aagtattatc
     7501 tacttgtcct acaaactggg gactagctcc aaacgatgct ttacaatggt taagagacaa
     7561 catgatacca tactatccat taggaaattt caaaaatgtt gaagttgagg aggtgaagta
     7621 aatgtctaca gctagagtaa tatgtgcagg cttcggaggc caaggtgtta tgtctatggg
     7681 acaattactt acttatgcag gaatgctaga aggaaaagaa gtttcttggt taccatctta
     7741 tggaccagaa atgcgtggag gtacagcaaa ctgtgcagta actgtatcta atgagccagt
     7801 tggttctcca cttataacag atgatgcaac tgcagctata ttattaaata taccagcatt
     7861 tgaaaaattc aaagatgatg ttattcctgg tggaaaaata atagtaaata gttctttaat
     7921 aaaagaaaaa gttgatagaa cagatgtaga tgtttattat ataccagcaa atgaattagc
     7981 agctgaatta ggaaatgata aagttgctaa catgataatg ttaggtgctt acttaaaagt
     8041 atctgatact gtagatatag aatctgtact agaagcattc aaaaaagttt tcggacctag
     8101 aaaagaaaaa ttcgttccat taaacagaga agctttacaa aaaggtatgg atgctacatg
     8161 ttctaaagca actaactaat aaagaaaaag atgtcaaaaa aaagacaata gaattatttc
     8221 tattgtcttt tttgctgata agtattaaaa tatttttatg actgaaatta agcctataat
     8281 gatattttat gaactataaa aatataatac aaattacaag cttttcaatt atgaaaggag
     8341 aatctttagt gagaaagtat tttggaacag atggagttag aggagtagca aatacagaac
     8401 taacatgtga tttagcatat aaacttggta gagctggtgg atttgtctta gctcaaggag
     8461 accacagagt aaaagtagta gtaggtaagg atactagaat atcaggtgat atgttggaag
     8521 catctttaat agcaggactt atgtctgttg gatgtgatgt tattactgtt ggaataatac
     8581 caacacctgc agtggcatat ttaactagaa aatatggtgc tgattgtggt gtggttatat
     8641 ctgcatctca caatcctgtt gaatataatg gaatcaaatt ctttaataag aatggatata
     8701 aattagatga tgaaattgaa ttgaaaatag aagaatatat agacgatata gacaaaattg
     8761 attgtcttcc aattggtgaa aatgtaggaa gaaaattaca tgaacattgt gcacaaagag
     8821 actatgtaga ctacctaaaa tcaataataa gcactgactt taagggatta aaagtagttt
     8881 tggactgtgc taatggtgcg tcatataaag tggcaccaat agtgtttgat gaactaggag
     8941 caagtgtaat ttcaataaat agttcaccag atggaaataa tataaactat aaatgtggct
     9001 ctacacatcc agaacaactt caaagggctg ttttggaaca taatgctgac ttaggtcttg
     9061 catatgatgg agatgctgat agattaattg cagtaaatga aaagggtcaa attgttgatg
     9121 gagaccatat aatgatattg agtgccttaa atttaaagaa aaataataaa ttggcacaag
     9181 atactttggt agttacagtt atgagtaata tagggttgac aatagctgct aaggaaaatg
     9241 gaataaattt atctactaca gcagttggag acagatatgt tttagaagat atggttaaaa
     9301 atggatataa tcttggtgga gagcaatctg gacatatgat atttttagat tacaacacta
     9361 ctggagatgg agtgcttagt tctctaatac ttgcaaatat aatcttgcaa gaaaagaaac
     9421 ctttatcaga aattgcatca ataatgagcc aatacccaca agtattagta aatgcaacaa
     9481 taaaaaatga aaataagaac aaatatatgg aatatccaga aataaaaact gagatagaaa
     9541 gaatagaatc tattttagac ggaaatggta gagttttgat aagaccatca ggaactgagc
     9601 cattagttag agttatgtta gagggtaaag aggaaggtca aataaaagaa ttagcaacaa
     9661 acttagctaa tcttatacaa gaaaaattat cgtaatttaa agggtattct cagatataaa
     9721 tactgaggac acctttctaa gcgccagaac ttagttatag acataattat tgagtaatat
     9781 aggaactaag ttgacgaggt cagagttaat cgaattgtcg gcggatgctc tgcggtgtgt
     9841 ttaccatcga aagacttctt taaataatag gagcaatcct atggacaaag ggaagccaaa
     9901 gtaaacttat atgacctaac tcttaaaata tataaataat atttttagga ggacacaaat
     9961 aatgtgtgga atagttggat atttaggaag tagaaaggca gcagaagtta tagtagaagg
    10021 gctttctaag ctagagtaca gaggttatga ctcagcagga gtagcagtta atagttctaa
    10081 tgaaaaagaa cttaatatta gaaagtttaa aggtagactt tctgtacttg cagaagactt
    10141 agaaaagaat cctattgatg gaaatttagg tatagggcat acgagatggg caactcatgg
    10201 agaaccatct gatgtaaact cacatcctca ttttaatcaa gccaaaacaa tagcagttgt
    10261 tcataatggg ataatagaaa actatatgga aataaaagaa gaattaattt ctgaaggtgt
    10321 taagtttgaa tctcaaacag atacagaggt aatagcacat ttagtagata aatactatga
    10381 gggtaattta ttagatgctg tatacaaaac tatatcaaaa ttaagaggag cttatgcatt
    10441 aggtgttata tgtaaagaac atggaaatga attagttgca gtaagaaaag atagtcctct
    10501 tgtagttggt gttggtgaag gtgaaaactt tatagcatca gatatacctg cattattaaa
    10561 atatactaga gatgtttatt tcttagaaaa tggagaagta gttcatctaa aagacgaaaa
    10621 tgtaacagtt tatgactcta atagaaatct tgttgaaaaa gaagtatttc atgtaacttg
    10681 ggatgtagaa gctgcgtcta aaggtggata tgactacttt atgtcaaaag aaattcatga
    10741 gcaaccaaca ggagtaagag aaactttaga aagaagactt gatgataatg gaaatataat
    10801 tctagacagt ataaatattt ctaaagaaga cttagaaaaa ataaataaag tttatatagt
    10861 ggcttgtgga acagcatata atgctggatt acttggtaag tatgccatag aaaaatttgt
    10921 aaatatacca gtaataactg atatagcatc agaatttaga tatagcgacc catttgtaga
    10981 tgaaaattca ttagtaatat tagttagtca atctggtgag acagcagata ctctagcagt
    11041 attaagagat tcaaaggcta aaggagcaag aatactttca ataactaacg ttgtaggttc
    11101 atctatagca agagaatcag atgatgtatt ctatacttgg gcaggacctg aagtagcagt
    11161 tgcatcaact aaggcatata ctactcaaat aacttcttta tacatgatag ctttagactt
    11221 tgctatcaaa aaaggaacaa taactagaga attttatgat agtatgattt ctaagatgaa
    11281 agaaatacca tcaaaaatac aagaaatact agacaatgaa gaatatataa aagaagttgc
    11341 taaaacagtt gttagttctg aacatgcatt ctatttagga agaggtatag actatagctt
    11401 agcaatggaa ggttcattga aattaaaaga gatttcatat atacatgcag aagcttttgc
    11461 agcaggagag ctaaagcatg gaacaatagc tttaatagaa aaaggaactc ctgtaattgc
    11521 catagctact caagaaaagt tatttgaaaa aatggtttct aatatggaag aagtaagagc
    11581 aagaggagct tatgtagtag caatagctca aagccataat aaggatgttg agaaagctgc
    11641 tgataaaata atctatatac caaattcaga tgatatttta tcaccaatat tagcagttgt
    11701 accaatgcaa ttacttgctt atcatgtctc tgtactaaga ggatgcgatg tagataagcc
    11761 aagaaatcta gctaaatcag taacagttga ataaaaacag attaaaaatt aaatagataa
    11821 tatttattga aaagaactca tcctgttatt tgtattttat aggatgagtt ttttaaataa
    11881 atattaaatt tgttttgttt ttgctataat aaattttata aaaagattga tattaaatca
    11941 tctgtaaaat gataaatata tagaccatac caggtatagg gaacgataaa tatagttcat
    12001 ataaaatata gtgagtactt aataaacata tagtcaatat cagataaaaa aaataatagc
    12061 ttatgagaaa aattaaaata caaggtatta gtaaccttgt gataagataa agtgagtagt
    12121 ttaaaataag aatataagga actatttata aaagaatata atgaagctaa taaagtataa
    12181 gaaaattact ttattttagt aaaataattt caaataaaaa ttttttaaat tgggagtata
    12241 aattatgaca aatgatttta aaaaagaaaa gaaagaagat tattttgtta atcttaaggc
    12301 aatatctact gaagttttac aagaaaaagt gcaagataac gcttgggtta tagttgatac
    12361 tcgcttaaat gatgcttata atggctggaa gttagatgga gtaaaaagag gtggacatat
    12421 aaaaggagca gtagattttt ctgctaattg gttaagtgta tactcagata gaaaagatga
    12481 agtgctagaa caagcattga aaactaaaag aattgattta gataaaaata tagtattata
    12541 tgatgcaaat ggaaaagatg ctttggttgt agcagattat ttatctaaaa aaggatataa
    12601 atatctatat aaatatgata ttaaacaatg ggcagacaac gaaaatcttc caatggaaag
    12661 atataaaaat tatcaaatga tagttcctgc ttttataata aaagatatat tagatggaaa
    12721 aattcctgag acatttgaag attcaaagaa tataaaaatg attgaagcga gttggggaga
    12781 agaatcgtat acaaaagggc atatacctac aagtgttcat gtaaataccg atattataga
    12841 acctcctcca acatggatgc ttgataatga tgataactta attaagtttg ctttagacta
    12901 tggattaaca aaggatgata ctgtaatagt ttcaagtagt acacctatgg catcttatag
    12961 actagctgtt atattgagat atataggagt aaaagatgta agagttttga atggtggaac
    13021 aaactcatgg ttatcagcag gatatgaatt agaatttact agtaatccaa aacattcatg
    13081 tactaatttt ggagcagata taccagttaa ctcacaatta gttgttacta cctctgaatt
    13141 aagacaaaaa ctaaaagaaa aaaataagtt tatactagta gataaccgta cttgggatga
    13201 acatatagga aaagtatctg gatatacata ttatgataaa aaagggcgta taccaggagc
    13261 tttatatgga cattcaggta gtgatagtgt ttcactagaa gaatatcgta atattgataa
    13321 tacaatgaga aataaatatg agattttaga aatgtgggac aaggaaaata tagatgtgaa
    13381 tagacagctt atatttatgt gtggaagtgg atggcgtgct gctgaagtat taacatacgc
    13441 aaatgtcata ggagtggaaa atacctccct ttatagtgat ggatggatgg gctggagtct
    13501 tgataattca aatttaattg aagtgggaga acacaaataa agtttttgaa agtacgatag
    13561 agttgtttaa aacaaaggtg tttttgattt attgggaaga gtgtgtaaat tgtgttttgg
    13621 gaagtgtact taggtatgtt catttggagc tatggaaatt gataaaaaag tattatctgt
    13681 ttattttgct ggtaaaatgt gaatagatta tagattatag attatagatt atagattata
    13741 gatttaagta tagactaagt aatttatatt acagaagaga atatataaga actagtgcaa
    13801 gatgtatttg atacatataa aaaattggca aatccagaat aaggatagag aaaattgaga
    13861 ttgaagagtt tgaaagagac ttattagatg aaaaatacaa agatgtataa aaatacatat
    13921 tgagaaagta tatctctctt aaatttgtta ataattctat tatacagaaa aacatattta
    13981 agggggattt tttactatga aaagagtaaa attagctata agctttttta tattaatgat
    14041 tatagggatg acaatatctt atgcagatac aagtataaat actaaataca atagattaag
    14101 taaagctctt gaaatttcag aagcaaattt taaattttat aatgttaaaa ttaattctgt
    14161 tataaatcat aatttatcaa ataaagaatt tcataatatt tttatagaaa taattaacaa
    14221 tctaaagttg gataaaaaag gaataaattg gaatcaaaaa gggtctggcg ataaaataca
    14281 attatgtgct actataaaag gtagcgataa agaaatttct ataaaaggaa taaataaaaa
    14341 taacaaagaa agttacatag tagttgacat attggataac aaagtatata aacataaaga
    14401 ggttatttat agtaacgtag aaaatgtgtt aaataaatat tcatctcaag tagatgtgtc
    14461 tgcttgtatt gttggagaat acacaaaaag gttacaaaac aacaaatatg atgatatttt
    14521 acaaaaaata ttatataata tgagtgctaa gcaaatagat gaagtgaaag acgtaaactt
    14581 tttatctata acagcatata gtaaagtatt aaatgattat gaattagaat atttagggga
    14641 aaaaattaat ttaaatatag gaattaaata taatgaggac gacggaagaa cactcatata
    14701 tattgccaca ccaattataa aattagatta ttaattatga ggagatcaaa aaatatggct
    14761 aagataatag taaaaaagag taatccactt aaaggtagtg ttaaaataga tggtgcaaaa
    14821 aatgcagttt taccaataat agcagcgact ttattagcaa acgggaaatc aacattaaat
    14881 ggagtaccaa atttaagaga tgttcatgtt atatcagatt tattaagaca tgtaggggca
    14941 gaagttgaat ataaagaaaa tacacttact gttgatgcaa gtaatataaa aacttgtgaa
    15001 gctccatatg agcttgtaag aaagatgaga gcttcttttt tggttatggg gccattgctt
    15061 gctagattta atagtacgaa aatttctatg ccaggaggat gcgctatagg aacaagacct
    15121 atagatttac atttaaaagg atttaaagcc ttaggtgcaa agatagaaat ggaccatggt
    15181 tttgttgaag cagcaacaga aaaattagta ggaaataaat tatatttaga tttcccatct
    15241 gttggagcaa ctgaaaacat aatgatggca gcttctttag cagaagggac tacaataata
    15301 gagaatgcag cagaagagcc agaaatagta gatttagcaa acttcttgaa cgaaatggga
    15361 gcagatgtta aaggtgcagg tactaatact ataaaaataa aaggtgttaa agagttaaaa
    15421 ggagcagaac ataatgtaat tccagacaga atagaagcag caacttatat ggttgcagca
    15481 gctatgacaa aaggtgatat aactgttgaa aatgttttaa tggagcattt aaaaccagtt
    15541 gtagctaaat taagagaagc tggttgtgaa ataacagaaa tggacaattc tgtaagagtt
    15601 gtaggtccaa aagtattaaa accaatagat ataaaaactt taccacatcc aggatttcct
    15661 acagatgttc aagcacaatt tatggctatg cttactgtag ctaatggaac tggtgtagtt
    15721 atagaaactg tttttgaaaa cagatttatg catgttgctg aatttaatag aatgggtgct
    15781 aatataaaaa tagatggaag aagtgcggtt gttaatggtg tagatgagct acatggtgca
    15841 gcagtaaacg ctactgattt aagagcaggg gcagccctta ttttatgcgg tcttatagca
    15901 gaaggagaaa ctcaaatagg agaaatatac catatccaaa gaggctatgt tgatatagat
    15961 aagaagataa cagcattagg tggtcaaatc gaaatagtag aagattaata attggtttat
    16021 agaagaaagt tataaattaa tagggttgtt catatactta aatacaaggt tttaaaagct
    16081 aaggaggaga gtatatgaag aacccattgg ttgttttgtt aggatttgtg acttgctctg
    16141 tgttagttcc atcacttata actttagttt cttataaaaa tgtagaatta actgaaaaag
    16201 cagagagccc agtatctata aacaaaacta ttaaaaaaag tgatattgaa gacaaaggta
    16261 ataaagaaga aaaaaataca gtgaactatg aaactgtaaa taaaaaagca ccaattatca
    16321 atgtatataa tcatataaca gggaaaaccg aaaagatgga catggagaac tatttatgtg
    16381 gagtactagc tggtgagatg tcttcagagt ttgatataga agccttaaaa gctcaatctg
    16441 tagcagctag aacatatgta gtatataaac aggaacatgg taaatctagt aaacataaaa
    16501 atgcagtagt atgtactgat tataaacatt gtcaagaata taagagttat gatactctta
    16561 aaaaattaaa tggtgaagaa tggattaaga ataaatattc caaaatccaa gaagcagtta
    16621 gaggaactaa aggtcaaata ataacttata acgataaggc gatacttcct ctttattttt
    16681 ctacatcttc aggaaaaaca gaaaatagtg aagaagtatt ttctgcaaaa tatccttatt
    16741 taaagtctgt ggaaagtcct tatgataagt attcccctaa atttgcgtca acacttaaaa
    16801 taagcaacac agattttgtt aaaagtctta gaagggcata tagcactatt gtgatagatg
    16861 tgaataattt aagtaaacaa gtttcaataa ctaaaagaag tgatgccgga actgtagaaa
    16921 aaataaaatt aggaaataaa gaattgactg gaaaagacat tagaactgtt tttaaattga
    16981 actctgcaaa ctttgacata aaatttggag aaggatacat agatttcgtg gtaaaaggat
    17041 atggtcatgg tgttggtatg agccagtggg gagcagaagg aatggcagaa gagggctata
    17101 aatattatga tatattatca cattactata cagatacaaa aataaaagat atatactaat
    17161 aattgtataa aatgccaaaa acacgtcaat aatccaaatg tagaaatata tttggaggtg
    17221 caaaatgaag aaaaagctgt tagaaaaaga tggtttttat ttatctttgt ttgtatgtgt
    17281 ttgtttatta gcagtaggtg gagtttggtt tacaaataat aatgtagata aattggcttc
    17341 taataaagga attatggaaa atgctaataa agatagtgaa gaggaaatac atttaattga
    17401 aaaagacaag aaagatgcta tccctactgc aacggattca aaacaaaact tagaaaaagc
    17461 taagtcaaaa gaggaaaata aatcgagtac aacaaaatta aattacattg gagataaagt
    17521 aataagagga tattcagaaa aagaacctag ttattctaaa acacttgatg tttgggaaac
    17581 tcataaaggt gtagatatta gttgtactaa aggcaaagaa gtaaaatctc tattaaacgg
    17641 aatagtagta gatgtattta atgatgaaga atatgggcaa tcagtcaaaa taaaaagtga
    17701 caataacatt gtagtagtat attcaaatct agataaaaat gttagtgtta agaaagaaca
    17761 gaaggtaaca gaaggacaat ctctaggtac tgtaggtagt acttctcaaa tagaaagtga
    17821 agaaggtata catgttcatt tagaggcgta tagtggagaa aaatctatag acccaatgag
    17881 tctaattaag taaaagtata acaagtttaa tcatatttgg ttaagcttgt tatatttttt
    17941 ggtgttttta catatatatc attaaagcta ttatgtgagg ggggtaatct ttgagatctc
    18001 atatagagga aagggccata gtggtagcaa aatatatact tgaaaaaaac actacagtaa
    18061 gacaaaccgc caagacattt ggagtaagta aaagtacaat tcataaggat gttacagaaa
    18121 gattgaagga aataaatcca tcccttgcca aagaagttaa aaatgtgctg gacaaaaata
    18181 aatcagagag acatattaga gggggattag caacgaaatt aaaatatgag aaggaacata
    18241 aaaaaatgta ggttaaaaac ctactttttt tatttaatta aattataaaa agaaatatat
    18301 taagtaatta taaatagcaa aataataata ctctcaaaag atttattttc tcttgattaa
    18361 agcgagattt ttgtatatgc tttattatag gagtattaaa ataggcttat aatataatct
    18421 aatattataa ataaaggagt tgaaaaaatg gctggagctg atataggcat agatttaggt
    18481 actgcaaatg tgcttgttta tgttagtggt aagggaatcg ttttagaaga accgtctgtt
    18541 gtggcgatag acaacagaac agattctgtt ttagctgtgg gagaagaagc taaaaagatg
    18601 atagggagaa caccaggtaa tatagtcgct ataagacctt taagagatgg tgtaatttca
    18661 gattatgatg taactgagaa gatgttaaaa tcttttatag ataagatagt tgataaaaag
    18721 ggatttggaa gattttttat gccaagaatt atggtatgcg taccaacagg tgtcacagaa
    18781 gtggaaaaga gagcagtaga agatgctacg agacaagctg gagctagaga agtatatata
    18841 atagaagaac caatagcagc agccatagga gctggtgtag acatatcaca gcctaatggt
    18901 aatatgatta tagatattgg tggaggtact gtagatatag ctgtaatatc tctaggagga
    18961 gctgttgtaa gtgaatctat aaaggttggt ggagatagat ttgatgatgc tatagttaaa
    19021 tatatgaaaa aacaacacaa tcttcttata ggagaaagaa cagcagagaa aatcaagttt
    19081 gaaataggtt ctgcatttaa gagagaagaa gaaaaatata tgaagataac aggaagaaat
    19141 ttaattacag gacttcctag ttctataact ataaattcaa cagaaatgtt ggaagcactt
    19201 agagaatgtg ttgagcaaat agtagttgct actcatgctg ttttggaaaa aacgcctcct
    19261 gagttagcag cagatatagg agatgttggt ataataatga ctggaggagg ttcattatta
    19321 tatggattag ataaaataat agaacagaga acaggaataa ctgttacaat agcagatgaa
    19381 ccattatctt gtgttgcaaa aggtacagga atagctttag gttcaataga cctacttgaa
    19441 actggcgggt ctttcaaaag aaaataatta ggagagttta gaatgttaaa tatagatgaa
    19501 ataaaaaaat taataccaca tagatatcct tttttattag tggacaagat tacagagcta
    19561 gaagttggga aaagagctgt tggtataaag aatgtaacag ttaatgaacc atttttccaa
    19621 ggtcactttc cagaatatcc tttaatgcca ggagtattaa tagtagaagc tttagcacaa
    19681 gtgtgtggag tggcaatgat gtctgtagag gaaaacaaag gaaaactagg tgtatttgca
    19741 ggtatagata aagttagaat caagagagaa gtaagaccag gagatacatt aactatggaa
    19801 gttgaaatga caactcttag aagaaatata gccaaggcag atgctaaggc atatgttggt
    19861 gaagaacttg tctgtaaagg ggaattaatg tttgccttag tagaaaaata aaaacttgaa
    19921 atataaaagt aaaaaggtag ataatttgga gtatttaatc agatactcta aaatatctac
    19981 ctttttatat aaagaatttt atcatttagt ttatatttag attaggtgga tactaataaa
    20041 gccaaggcta ttgtttcagc taagtctaat ataaagctaa gacgtatatt attaaatgaa
    20101 agtttattat tttcatcaat tttatctaca attcctacta tagaataatt tcctatttga
    20161 ggtagttttt tgccaacacc ttttccgggc aggataggtc cttctctaat ctgtatatta
    20221 cctatgttac tctgcgaccc caagcaagca tctatagcca agaagttacc ttgtatatgt
    20281 gtatttttta tagtatctaa agactcatat atatttaagg cgtgaatagg gttatctagt
    20341 gtaccgtaaa cggggtattt aaagttactg ttcttaagta ttgttccaac taaagggcct
    20401 aaagtatcac ctatagccct gtctgttcct atgcaaacaa caatcgtatt ttcattaatt
    20461 atagttttta gaactgaact tagcatctgt ataacatcat catcgctgta atttacttta
    20521 gctaaataca ataggaacac ccccttcaaa aaaatatatg aataaaaagc attaaaaaga
    20581 cctaacttta aatttcagat agatagaaaa ttttatttgt cttacaaaaa aaggaataga
    20641 aaaaatatga ctaaaaatat tgacaaaatt ttaaaaaaat agtaagctag taaagtaaaa
    20701 caaaacttaa atcttgatta ccttatcaag agaggctgag ggataggccc tgtgaagccc
    20761 agcaaccacc caaatgggaa aaggtgctaa ttcctacaag acaaaagtct tgaaagatga
    20821 ggtcagttaa ttagtatgta attacgatta ttaacctctt cttataaagg agaggttttt
    20881 tattttgagt aaatttctaa aattaaaaat aaggtaaaac ctataataaa aactaagaat
    20941 aaataagggg gaataaaaat ggcaagacat ttatttacgt cagaatcagt tacagaagga
    21001 catcctgata agatatgtga ccaaatatca gattcaatat tagatgcatt attagaaaaa
    21061 gacccacaat ctagagtagc ttgtgaaact acagttacta ctggtttagt tttagtagca
    21121 ggagaaataa gtacatctgc ttatgtagac attcctaaat tagtaagaga aacagtaaga
    21181 gaaattggat atacaagagc aaaatatgga tttgattgtg atacttgcgc agttataact
    21241 tctattgatg aacaatctgg agatatagct atgggagttg atgaaggcct agaaagtaag
    21301 actggagaag agatagaaga agaaatagaa aaagttggag ctggagacca aggtataatg
    21361 tttggatttg catgtaatga gactccagaa ttaatgcctc ttccaatatc tttggcacac
    21421 aaattatcaa gaagacttac agaggttaga aagactggtc tagttgatta tttaagacca
    21481 gatggaaaaa ctcaagttac tgttgaatat gaaggaagta aagctgtaag agtacataca
    21541 gttcttatat cagctcaaca ttgtgaaaca gtatcaaatg ataaaataag agaagattta
    21601 attaatcatg ttattaaaga agttatacca gcagaattac ttgatgaaga tactaaaatt
    21661 tatataaatc caacaggaag attcgttata ggaggaccac aaggagatac aggtcttaca
    21721 ggaagaaaaa taataataga tacttatggt ggatattcta gacatggtgg aggagctttc
    21781 tcaggaaaag accctacaaa ggttgataga tctgctgctt atgcagctag atatgttgca
    21841 aaaaatattg ttgcggctgg tcttgcagac aaatgtgaga tagaacttgc atatgctata
    21901 ggtatagcta gaccattatc tatatttata gatacttttg ggacaggcaa agtttcagaa
    21961 gaaaaacttg ttgaattagt aaataaacac tttgatttaa gaccaggagc cataataaga
    22021 gacttagatt taagaaagcc tctttataaa aaggttgctg cttatggtca ctttggtaga
    22081 actgatatag atttaccttg ggaaagaact gacaaggttg agcagttaag aaaagatgct
    22141 ttaggtgaat aatttttaac taaaaagctg ttagaatgta attttacgat tactttctaa
    22201 cagctttttt attatgagtt aaatattttt aataaatgtt aatttatcat catataatga
    22261 ataaacagta tttaatctgg tcaatacatt ttatataata ctatactaaa tttatgcata
    22321 ttatgggata tatgttaaag gatggttaag aatgtgttaa ctaatgttaa ataattaaaa
    22381 atatattgaa atatcataat aatgagtcta atataatctt aataaactta cactttaaaa
    22441 ataattttaa ttggggggag tagtgtatgt caggcaaagt aaatattaaa acggttataa
    22501 gttttgctgg agcttatgtt gctactgtaa taggttctgg atttgcaact ggtcaagaaa
    22561 tactgcaatt ttttacattt tatggatatg ctggtattat aggtggaata atctctatgg
    22621 tactattttc atggtttgga gcagaggtta tagataaggg aagagaattg aaacttaagg
    22681 agccaataaa aatttatcaa gtatattgtg gaaaatatct tggaacattt tttgaatggt
    22741 ttggtccatt gtttttattt ggaacattag taataatgat tgcaggagca ggagctacat
    22801 tatcagagta ctatggatta aatccatatg taggtaggat tggtatgaca atcgtttccc
    22861 taataactgt ttcattaggt ttgacaaggc tttctaaaat actaggtaat ataggaccta
    22921 taataataat atttacactt ttagtaggag caattagttt atttagcaat attgatgctt
    22981 tatcaaatgc tggaaatatg gttgatagtt taaatattaa aactgctaca tcaaatggat
    23041 atttctcagg agtactttat acttgttata atgtaataat agtaataaca ttcttgacag
    23101 gaatgggagc ttctgcagta aataagaaag atgctgtgtg gggtggtata gtaggtggag
    23161 tagcacttat ggcagcagct ataatgatga atttggcatt actttcagac ataggaaata
    23221 tatacacaaa agagatacca gcactttact tagctgataa gatatctcca ataataggta
    23281 ttttattctc agtagtactt ttactaggaa tatacactac agctgtacca ttgttatggt
    23341 cagtaactaa ccgatttgta gaagatgacc accctaaatt taaaattata acaatagtag
    23401 taagtatatt agcttgtata ggaggtctac tgccatttga taagttagta ggtacactat
    23461 atccatatac aggttacatg ggaattttaa ttttactatg tatgctttat agaagaatta
    23521 ctaaaactga ggggtacaag gagaataaat ctgaaataag ctagtactgt aatatactta
    23581 tgaaatttaa tttatactct aaatatggag taattaaaat aaaatagcta tttaaatatg
    23641 gtacctgtat ttcatggtac catattttat ggatgcagat tttttttgtt tctgataaaa
    23701 tgtataatag ttggaaatta cgttaagtat aaaaatataa aacttttcta cgattatcat
    23761 agttttaata tataaaatta tactaacatt taaatggagc gattattagt atttttagga
    23821 gataaaaatg gaaaaattag aaggaatgat aagtgaaata gtctttaaaa acgaagataa
    23881 tggttataca atagctcatc tggtaaatga aaatgatgaa attgtcgttg taggatgtat
    23941 gcctactttg gcaattggag aaagcataga agttgaagga aagtgggtaa accataagat
    24001 ttatgggact caatttgaag tgaatagttt tatgccagta acaccatcat ccttagaagg
    24061 aatttatgtt tacttatctt ctggaatgat acatggtatt ggtgaaaaaa tggctaaaag
    24121 aataattgat aagtttggtg tagatacgtt agaggtgata caaaattcac cagaaaaact
    24181 acaagaagta gaaggtatag ggagtaaaaa agtaaaacaa atagtaaaaa gttatgaaga
    24241 agacagagaa ttgagaaata taataataca gctatctcct tttggaataa caccaaatta
    24301 ctgcctaaaa atatataaaa aatataaaag cagtgcaata gaagttataa ataaaaaccc
    24361 ataccaactt gctgaagata taagaggaat tggatttaaa gtagctgata gtatagcaaa
    24421 taaaattggt atagataaaa attctaagga tagaatttgt cagggaatct tatacacttt
    24481 aaataaatct ttaagtaatg ggcatacata tttaccagag catgttctaa tacaagattc
    24541 ggaaaaatta ttggagctaa atggagaaat aataaaagag tgtgtaatga tgctagttta
    24601 taatcaaaaa atacatgtag agaaagtaaa caatgaaaat cttatatacc ttatgccata
    24661 ctatttagct gaaaatggag tatgtagtca aatagtaaaa ttatcacagt atgaatttga
    24721 agacttaaaa atagatatag ataacgagat taatgtttta gaagaagata agaaaattaa
    24781 gttagcagaa aaacaaattt tagcagtcaa agaatctgtt aatagtggcg tattaattat
    24841 aaccggtggt ccaggaactg gtaaaacaac taccattaat gctatcatag atatatttga
    24901 aaataatggt aaaagcgtga ctttggcagc tccaacggga agagcagcaa agagaatgag
    24961 tgaaacttct aataaagaag caaaaactat acataggttg ctagaaatgg gattttcaac
    25021 agatgatgat ttaacttttt ttaaggatga agaagaccca ataaattcag atgtaataat
    25081 tgttgatgag gtatcaatgg tagacattat cttaatgtac aatttactta gagctataaa
    25141 gttaggaaca agagtaattt tagttggaga tagtgaccaa ttaccttctg taggtgctgg
    25201 aaatgtactt aaggatatga ttaattctaa tattataaat gtagttaaat taaatgaaat
    25261 atttagacaa gcgcaagaaa gtatgataat tgtaaatgcc cataagataa acaatggaga
    25321 accactttat ctaaatacaa aaggtaaaga tttctttttt ataagaaaat caactaatga
    25381 agaaatttta aatgaaataa taggattggt taatgaaaga ttacctaaat tttataaagt
    25441 tgataaatta aaagatatac aagttttatc ctcaatgaga aaaggtgaat tgggagttac
    25501 taatctcaat atagagttac agaagtatct taacaaaaaa gaaaaattta aagttgaaga
    25561 aagtttttct aaaagattat ttagagttgg cgataaggta atgcaagtaa aaaataatta
    25621 tacaaaaaaa tgggaaacag aagaccaaaa agaaagtgga gaaggtatct ataatggaga
    25681 tataggatat gtttatcaca tagataaaga taaaaaaact atttatatat tatttgacca
    25741 gactaaaatt gtatcatacc tatatgatga attagatgaa attgaccata gtttttgtac
    25801 aacaatacat aaaagccaag gaagtgaatt tccagttgta gtattgccaa tagcttgggc
    25861 tcctcctatg ttacttagcc gtaatttatt atatacagca gtaacaagag caaaaaaatt
    25921 agttgtatta gttggagatg tgaaatatct tgaatatatg ataaaaaata atagagttaa
    25981 tcaaaggtat tcaaatttag gatataagct aaataaattt aagcaagaag ggctgttgat
    26041 agaataaata tgaaaatatg caatgataat tttattaaga attttaaaaa tatgattaat
    26101 aaatgtttgg attttatata tcctgagaat ataagttgca taatttgtga taaatccata
    26161 aagaaaacaa atacttattc aatatgtaaa agttgtttta aagaaatgaa ttttatacaa
    26221 gatggttgca tgaagtgtgg aaaaccaata atacgtcatt caattgaaaa agaatttatt
    26281 gaagaatgta gttattgttt taataaagac ttttattttg ataaatcaat atcttgtatt
    26341 gaatacaatg atgtgacaaa aaagatagtg ctagggctga agtataacca aaaaaccttt
    26401 atggctaagt atatagctca aataatgaaa gaaaaattat atttagaaaa cataaaattt
    26461 gattatatat tatttgttcc acttcataaa aaaaggctaa ataaaagagg ttttaatcag
    26521 gcacaaaaaa tagcatttaa tttaagtaaa atagtaaata ttccattatt ggattgtata
    26581 agtagaaaaa aatatacaag aatgctatat aaattgaata agaaagaacg aaaagaagaa
    26641 ttaaaaaatg tatttgttgt taaagacaat gttaaattaa taaataataa aaatatactt
    26701 ttaatagatg atatatttac tactggtcta acaacaaacg aaatttctaa acttttaaag
    26761 ttatcgggtg ctaataaggt ttttgtattg actctactta caaaagcaaa tgataattat
    26821 attatggaat aatcttatat attgacaatt ttaatttaaa atattaaaat gaataagtga
    26881 tatttaggtc tgtggttgaa agtccaagcc agtcgcaggc aaagggatcc acgtaagtta
    26941 acactaaaaa ttgggttaat gatcatggtg cggcttagaa gtaagaccta ccgataataa
    27001 aatcgagaga gagtagtagt gcggcgaacc caagcgaacc ctccagtagg cgagtgtggg
    27061 gtcaaaaacc aggtcagcta aatatcgtat taaaagggag ctagcatacc tttatgtata
    27121 gctctttttt atatataata aatttaggtt gagtcataaa gaggtgatat gtttgaataa
    27181 aagacaggaa aacttattaa caacgttgct attaaaagaa gagtttataa atgttaataa
    27241 gattgccaat gattttgagt gttcagaaag gacaataaga aatgattgca aatatattga
    27301 tgaatggtta tctacttttt gtaatgctta tatcaaaaga aaacctaata ttggaattaa
    27361 atttagtgga gatggaacag acaaaatact ggtaaatcag aagttgcgag gtgaagttaa
    27421 aagagtaaat agtgagcttc ataagcaagt tgagatatta aatttactac tttgtaataa
    27481 taaaaaagtt actttaaatg atttatctaa taagttgtac ataaataaaa atattgtcag
    27541 agaagaaata agtaaattat cagtggcatt aaataattat aatttaaatg tctcgactaa
    27601 aaaaggcagt ggtatatata ttggagggga tgagaaagac ataagaataa tgttagtatc
    27661 attcttattt aaatacatag ataaatacag attaaacata gatgaaatag agtacttcca
    27721 tatagtagat ataataattg taaaaaacat aatgtttact atagaggagg atatggatat
    27781 aagacttaca gatatatcat tcaagcaatt gatgctattt ttacttataa atataatacg
    27841 aataaggcta ggaaattctt taaaagtatc aaatactaag aaagagctta agctaaaaat
    27901 gctgatatta gatatagaag atgaattgaa atctaattta tccatagtat taaattatga
    27961 agaaaaattg tttatagagt ctttaatatt gggtatgaac aaacaaataa atagtagtaa
    28021 tattaagaaa tctttatatc taaatgaaga attagcaagt tatacaaaaa aaattataaa
    28081 tttagtatca gaagaatccg gaataaattt taataatgat atattacttt atgaacaatt
    28141 aatatgtcat ttgaatgtta caatgcacca aatgaaaagc aatgtttact tagaaaatcc
    28201 acttttagat aacataaaaa ctaaattttg ttttttattt acagtaatat caagttcaat
    28261 agaaattgaa ttaaaaggaa aacaaataac agaagatgaa attggttatc ttacacttca
    28321 ctttcaaacc tctctagaaa gaaaatacag caaaagggaa tcaaataaaa aggcaagtat
    28381 tgtatgtcca tttagttttg gagtaagcat gctattaaaa gtaaagatag aaaagagatt
    28441 tgataacata aaaattattg aaacattaag agaggaagat ttaaaaagag ataacttcaa
    28501 taagactata gattttataa ttgcatttca agagtatgaa aatatccaaa aaccaatttt
    28561 tataacaaca ccattattta ctgatgagga tgagaataaa cttaaagagt tcacaaacaa
    28621 aataaaagaa aaagacactt catataaact tatgaacaga ttaatgatgt ctgattattt
    28681 aataagagag ttagaacatg atgatattta tgaagctata acatatttaa ctaatttatt
    28741 aattgaaaaa cattatgcag aaaaagagta tttacaatct attataacaa gagaaaagtc
    28801 atatccaacc aatgttggaa aaggaatatt attccctcat ggagatatga aatatataaa
    28861 acaatctgtt ttatgttttg caaggttaaa aacaccaata agaattagaa atggaaagga
    28921 tataaagatt atacttttgt tagcatataa aaaagatgat gatagaaaag tatttagaga
    28981 attatttaaa gaaatatcta atttaacaga ggatgaaaat atgctagata ttctaagtaa
    29041 atgtgacatt gaaaaagtaa aagatattct tatataaaat tcatagtgaa ctatggataa
    29101 gcacaaaatt ttccatgtga tggaaaaagt tgtgcatgtt tttttgtttt tataagatat
    29161 tatatgtttg taaggtataa ataatctaaa agtagagaag caacttagga ggaaaaaatg
    29221 gaagaaatag ttttaaagat tataattcat gctggaaatg caaaatctat gctttatgaa
    29281 gcacttgact atgcaaagga aaacgatttt aaaaaagcag atgaattaat tgaaaatgca
    29341 aatgaagaaa ttttaaaagc acataaagta caaactgaac ttatacaaaa ggaagctggg
    29401 ggagataaat cagatatatc tattttattg attcactctc aagaccattt gatgacttgt
    29461 atgagtgaga gaaaccttat aaaagaaatg attatgttaa gaaaagaaat tcaaaaatta
    29521 aaataaaaaa attaaggggg atttaaaaaa tgaaaagaaa agtatattta ttttgtagct
    29581 ttggtatgtc aacaagttta cttgcagata aaatgcagaa ggtagcagat gaacacaatt
    29641 taccaataga agtagaggca tttccgatag cagaaattga caaaatagtt gaagaaaaaa
    29701 atccagattg tatattacta ggaccacaag ttaaatacat gctaaaggaa ttaaaaccaa
    29761 agtttgaagc tcaaggaaaa cttatagata taatcaatga agttgattat ggaactatga
    29821 atggggaaaa agtattaaaa ctcgctataa agctaataaa atccaaacaa gcgtagaatt
    29881 atagtaaaaa ataataggaa atgagggtag atagatgaat agattggaaa gaatacttat
    29941 gccattggct gaaaaaatag gtaaaaataa attattaatt gcaattagag atggtttttt
    30001 aatatcatca ccattattaa taataggttc attattttta ttagtagcaa attttcctat
    30061 aaaaggatgg aatgaatttg taggtcaaat ttttggttca gattgggctg taaaattgca
    30121 acaaccaact gtagcaacat ttgaagtaat gactctactt gcagttttag gaattggata
    30181 ttcttatgca aagcaaatag atgtagaccc aatagcatca gcagcagttg caatagttgc
    30241 cttttttata ataacaccat ttgtaatacc atatacacca gaaggaacag aatcagtcta
    30301 catggtaaca ggtataccac taggttggat gggctctaag ggaatgtttg taggtatgat
    30361 aacagcaata acctctgtaa aattatttgc tgcagttgta aaaaaaggat ggacattgaa
    30421 aatgccagat ggagttcctc caaccgtatc aaaatcattt gcagcattaa taccaagtgc
    30481 aatagttatg gtagttttct ttttagttaa gatagctttt gaagctacac catatggaag
    30541 tatacatgaa tttatattta agtttttaca aatgccatta ttaaaattag gaaatagttt
    30601 aggtgctatg gcaatcgctt atatattctt acatttattc tggttctttg gaataaatgg
    30661 aagtagtgtt gttggggctg tgtataatcc aatattaaaa attctatcag ctgaaaattt
    30721 agctgctttt caagctggtt caaaaatacc aaacataata acaggtcaat tccaagatat
    30781 gtttgcgaca tttggtggag caggtagtac actatcatta gtcatagcta tgttattaat
    30841 atgtaaaagt aaacgtataa agtcaatggg aaaactatca attttaccag gaatatttgg
    30901 tataaatgag ccattgatat ttggtctacc aataatgctt aacccaatac tattgatacc
    30961 atttgcaata gttccaacaa taaatataat aatagcttat ttctgtatgt cagcaggatt
    31021 agtgcctctt acaaatggag ttcagttacc gtggacaact ccaattatat tctctggttt
    31081 tttaacaaca ggatggcaag gtgcagtctt acaagtagtg cttcttatac taggaatatt
    31141 tatgtatata ccatttataa aaatgctaga taagcaatac ttaagagaag aaaatgcaac
    31201 tgttgaagag gaagatgatg atatatcttt tgatgattta gtattatagg aggaagatga
    31261 tgaagaaaat agttatgata ttggatcaaa tacaagctgg agctggagga aaagagaaaa
    31321 gtaatatacc tcctgctgga aaatcaagcc cattaggacc aggtataatg atggagcaat
    31381 ttttaaatga atctaaggta atagctacct tattttgtgg ggatgaattt tttgtaaata
    31441 atgaagaaga agtaactagt aagatgatag ctatggtaaa aaaattaaac cctgatgtag
    31501 taatctgtgg accaagcttt aattacgaaa acttttctaa gatgtcagca atactgtcta
    31561 aaaatataaa tgataaaaca gatataccag cttttgcagc tatgtcagaa gaaaatatag
    31621 atgtgataaa cgaatataaa aatgacatat gtatagttaa gactccaaaa aaaggtggaa
    31681 ttggtcttaa tgattcatta aataacatat gtaaattggc aaaagcaata gcaaataaag
    31741 aagatataac tttgatgaaa gaagaatttt gttattaata agagtattaa ttattagtaa
    31801 gtttaaatat tatactattt ataataagca tacattagta aaatctatat aatttaagga
    31861 ggggaatttt aacatgtggg gaatgatagc gacatggaga atggccctag aaggaataac
    31921 tgaggcatca gaacatttag aaaaaggcat ggaagcaggt gatgctgtag agcttgctgt
    31981 aagaagagtt gaagactatc cattctataa atcggttgga tatggtggac ttccaaatga
    32041 aaattgtgag gtagaacttg acgctgcatt tatggatggt aacacattag atattggagc
    32101 agttgctggt ataagagatt attctaatcc tgtcagcata gctaggaagt taagttatga
    32161 gaaggtaaat aattttttag ttggaatagg tgctgaagat tatgctcata aaaatggatt
    32221 tgaaagaaaa aatatgttga ctgatagagc gaagttacat tataaaaaga gaaaaaaaga
    32281 gactttagac aaaggattaa gtccatatgc tggtcatgat actgttggta tgatatcttt
    32341 agacaaaact ggaaaaatgt gtgcagctac atcaacaagt ggattgttta tgaagaaaag
    32401 aggtagaata ggagattctg cactatctgg ttcaggattc tatgttgata gtagtatagg
    32461 aggagctaca gctactggtt tgggagaaga tttaatgaag ggatgtatat cttatgagat
    32521 agttagactt atgaaagaag ggaagcatcc acaagaagca tgtgatattg cagtaaatga
    32581 acttgataaa cagcttatca aaagaagggg aaaagctggt gatttatcat tagttgccat
    32641 gaataataaa ggtgaatggg gagtagctac aaatatagct gattttactt tttcagtagt
    32701 aactaaagaa gaatctccta ctgtatatat ttcaaataga gtagatggaa agactataca
    32761 taaggttata tcaaatgagt ggatggaagc atatgaggct agaataaaag caccaataga
    32821 agtaagtgag taaagtagtg gttacataat taaaatagag ttttataata aggagatttt
    32881 tatgaatatc ataagtaata aaatagatga gttaaaagaa gatttattaa gtgatattat
    32941 agatattgta aaaataccta gtgtcaaagg ggagtcagaa aatggcttcc cttttggcga
    33001 aaaagtaggg gaggcactta ataaagcatt agaaatatca gaaaaattgg gatttaaggt
    33061 tagaaacttg gataattata ttggttatgc agagcatgga gatagtgatg attatgtttg
    33121 tgtaattgga catgtagatg ttgtacatga aggagatggt tggaagcatc agccttataa
    33181 gggagaagaa actaatggaa gaatttatgg acgaggagta ctagacaata aaggacctat
    33241 aatgtctgcc ttatatggat tatatgctat taaagaatta aatttaaaat tagataagag
    33301 agttagaata atatttggta caaatgaaga aagtggattt gaagatatac catattattt
    33361 agaaaaagaa aaagcaccta ttatgggatt tacaccagat tgtaaatatc ctgttgtata
    33421 tggtgaaaaa ggcatggcaa agattagaat aaaaagtaaa atcaattatg aagaagatgt
    33481 atacttaggt tttatagaaa atatgtctga aaatgtgctt gttacatata aagaactgaa
    33541 tatagaaaat agtgatacta tattagatat aaaggtaaaa tacgatttta gttataaatt
    33601 gaaagatgta ttggatgaaa taaaagctag ttttccaaat agtatcgata tagaggtaat
    33661 ttctaatttt aatcctgtat attttgataa agagagtaat cttgttaaaa agcttcagct
    33721 agcttatgaa agggttacat cattagatgg aactccagta acaactaatg gaggtacgta
    33781 tgcaaaagta atgccaaata tagttccgtt tggaccttca tttccaggac aaaaaggaat
    33841 agctcataat cctgatgaat acatggatat agaagatata atattaaatg caaagatatt
    33901 tgcaaatgca atatatgaat tagcaaagga gtagttatgt tagaaataat aggaatgtca
    33961 gtagaagacg ctaaaattat agaagattgt ggtgcagata gaatagagtt agttagtgct
    34021 ttaactgaag gtggattgac accaagtttt ggtcttatag aaagtgttgt taacagtgta
    34081 aagatacctg tgaacgtaat gataagacat catgcgaaaa gttttgtata cagtaaagaa
    34141 gatataagta taatgcaaaa agatataagt gtggttaaag agattggtgc taatggagtt
    34201 gtatttggtg tattggataa aaacaatagt atagatgaga aaaatctaaa tgttttattg
    34261 aaatgttgtg ataatcttga tgttacattt catagagcaa tagatgaatc aaatacaata
    34321 gatagtgtta aaattttgaa ggattatgat aaaattacga atatattaac atcaggaggg
    34381 aaaggaagta tagttgataa catacaaatg ataaagaata tgatgcttag ttctaatcac
    34441 ataaaaattc ttttaggtgg aggtcttaat tttaataata tagaaaagat aaaggaatta
    34501 actaaggcaa gtaattttca ttttggtaca gcgattagaa taaataacag tccttttgaa
    34561 gatatagata gacaaaagct taaacaactc gtaaatataa tttcaagata gtatatgaat
    34621 ataaaatact tagtttaaat aaagagagta agttaattat aatttgggta tatgaatgtt
    34681 aataggtatt aaatatgaaa ttattgttga gtatgcttta taacttttaa gtagttttaa
    34741 atataaataa tgaaagaaaa gatagattat ggaggtttta gagggtttat atttattaaa
    34801 aaattataaa ggtatattgg catataatac acataatttc ctaaaattac catcttatga
    34861 catgaattca catattaata aacaattaat ggagattttt gtggatagaa ataggagtaa
    34921 aaatagcttt aaataaacaa aaacaagagt tattaacagt tttatccaca ttatccacag
    34981 gtgtttgtaa taatattaat ccacataagt atcaatgtaa ctaaaaatga taaataattc
    35041 tatcaattga atagaaaaaa tcataaaagt aaataataaa aagaggatta tgagagttcg
    35101 tgtagaatat attattaaag tataaatttt cttatactgg cataattgaa gggagatgta
    35161 tttatgaaca taattatatc tggaaaacaa atcgaattaa ctgatgcaat aaaaaataaa
    35221 atcgaaacta aactaagtaa gttagataat tacattcatc cagacactga tgtaaaagtt
    35281 acagtaagtg cgagaaaagc aagacaaaaa atagaagtaa caatagctac tgtaaatggt
    35341 cctataatta gagcagaaga tatgcaggaa aatttataca cagctataga tgttgtatat
    35401 gataaattaa gtaaacaatt aaaaaaatat aaaaagagat tacaagataa acataaagac
    35461 aacaagagta taagattcca agatgcagag ataaatctag ctgatgaaca agaggattat
    35521 ggggatgaaa atcaagaatt agtaattcaa agacgtaaga aatttagtgt taagccaatg
    35581 agtgaagaag aagctgtatt acagatggaa ttaataggac atgattttta tatgttcaag
    35641 aacatagatt cagatgaaat agcagtagtt tataagcgta ataatggtgg atatggtata
    35701 atagaacatg aataaagcaa tttagattta atcatattag ctatcgataa ataaattatc
    35761 gatagctttt taaattaata ttaaaagtat agtttttatg gtaaaatgaa taatagtaca
    35821 ataaagaacg aggagagatg taaaatatga gctttatgga taatttattc aacatggcag
    35881 ataaaaaaga attaaaaaag ttcaataaaa ctgttgatat aatagattca ttagaaccta
    35941 aatttgaatc tatggcagac tcggaactta aaaatatgac aaatatattt aaagaaagat
    36001 tagcaaatgg tgaaagtata gatgatatac ttccagaagc ttttgcagta gttagagaag
    36061 tttctaagag agtgttagga cttagacact acagagttca aatgattgga ggtatagtac
    36121 tacatcaagg aagaatagca gaaatgaaaa caggtgaagg taaaactttg gttgcaactg
    36181 ctcctgttta tttgaatgcg cttactggaa aaggtgtaca tgttgtaaca gtaaatgatt
    36241 accttgcaaa acgtgataga gaccaaatgg caaaaatcta tgagttttta ggaatgagtg
    36301 taggcgttat tatacacggt caaaatccaa aagttagaaa agaacaatat gattgtgata
    36361 taacttatgg tacaaataat gaatatggat ttgactatct aaaggacaat atggtaatac
    36421 acaaagagca aagagttcaa agaggattaa attatgctat agtggatgag gttgactcta
    36481 tactaataga tgaggctaga acaccactta tcatatctgg acctggagac aaatcaactc
    36541 acttatattc agatgcaaac acatttgttt taaccttaaa accagatgat tatgaattag
    36601 aagaaaaaga taaggctgta tctctaacag catcgggtat acaaaaagca gaagtgtatt
    36661 ttaatgttga taatataaca gatatatctc atacagaatt ataccatcat ataaatcaag
    36721 ctttaagagc acatgtaata atgaagaaag atgttgatta tgttgctaaa gatggagaaa
    36781 tagtaatagt agatgagttc acaggtagac ttatgtttgg tagaagatat tcagaaggtt
    36841 tacatcaagc aatagaggct aaagaaggtc ttaaaataca aagagaatct aaaacactag
    36901 ctactgtaac attccaaaat tattttagaa tgtacaaaaa actttctggt atgactggta
    36961 cagcaaaaac agaagaagaa gagtttaaag caatctacaa aatggatgtt ttccaagtac
    37021 ctactaataa gcttatgata agggaagact taccagattg tgtatacaaa agtgaaatag
    37081 gtaaatttaa tgctgtagca caagaaataa ttgaaaggca caaagtcaat caaccaatac
    37141 ttgtaggtac agtatcaata gaaaaatcag aattattatc acaaatacta aagaaaaagg
    37201 gcataaagca cgaggtatta aatgctaagc atcatgataa agaggcagaa ataatagcac
    37261 aagctggtag acttggagct gttacaatag ctactaatat ggctggtcgt ggtacagata
    37321 tagttctagg tggaaatcca gactttttaa ctaagagaga aatgagaaga aatggattta
    37381 aagaagaaat agtaaataga gtagatactc caatagaagg tatacctgta aaaggtaatg
    37441 aaattctatt tgaagctaga gaagagtatg aaaaactatt tgaaaagttt aagcaacaaa
    37501 cacaagaaga acaaaaacaa gttgtagaag caggtggact tgctataata ggtacagaaa
    37561 gacacgaatc tcgaagaata gataaccagt tgagaggtcg tgctggacgt caaggtgacc
    37621 cagggagttc aagattctat ataggtcttg atgatgacct tatgagattg tttggtagtg
    37681 atagaatatc tggtattgta gataaaatag gtcttgaaga agatatgccg atagaacata
    37741 gaatattatc taagtctatt gaaggagcac aaaagaaagt cgaaggtaaa aactttggaa
    37801 taagaaagca tgtacttcaa tatgatgatg ttatgaataa acaaagagaa ataatatatg
    37861 cagagagaaa acgtgtatta gaaggtgaag atttacaaga acaaatacaa tcaatgactc
    37921 attctataat agaagaagca gttactcttt atacacaaga taaaggattt gatgaagaag
    37981 gatttaaaga acatatgtat aatctgttct tgccaaaagg tagcatagaa ataccagaaa
    38041 tagaaaaact taatccagtt gaaataacag aaaaagttta tgaaatagct atgaagatat
    38101 atacttcaaa ggaagaacaa gttggctatg agagaatgag agaagttgag agagtaatct
    38161 tacttcaagc tgttgataac cattggatag accatataga tgctatggac caattacgcc
    38221 aaggtattgg tcttcgtgct gtaggtcagc aagacccagt tattgcttat aaaatggaag
    38281 gttttgatat gtttgatgaa atgaataaac atatcaaaga agatactgta agatatttat
    38341 ttaatataac aatagaaact ccagtggaaa gaaaagcagt tgtagatgtt gaaaatctat
    38401 cttctccatc agatggaaca cttccaacaa gtaaaactgt taaaaaagat gaaaaggttg
    38461 gaagaaatga cttatgccct tgtggaagtg gtaaaaaata taaaaactgt tgtggaagat
    38521 aatctcggag gattaaatgt taaagttaca agagtataga catagtatgg agcagatggc
    38581 ttcaaattta gaagaattga gggtttctct ttgacatagc ttcattgatg aaaaatatac
    38641 aaattaatga agaaaagatg aataaacaag acttttggaa tgataatgaa gtcgctcaaa
    38701 gagtacttca agaaaataag tctttaaaag agacattaga agaatatgaa agcttaaaaa
    38761 gcttactaga agatatagaa gtattaattg aaataggttt agaagaagat gatgattctg
    38821 tagaaagaga tatagaaaag tccatagaat caatggaaga aaaattatct gaaatgaaaa
    38881 taaagactct tctaaatgga gagtatgata aaaataatgc tatattatct ataaatgcgg
    38941 gtactggtgg cttagatgct caagattggg ctcaaatgct tttgagaatg tatataagat
    39001 ggtcagaaag taagggttat aaagttaagc tcttagatat aatatcagac cctgaagcgg
    39061 ggataaaaac agctacaatt cttgtagaag gaacaaatgc ttatgggtat ttaaagagtg
    39121 aaaaaggagt tcatagatta gtgagaatat ctccatttga cccatcagga aaaagacata
    39181 cttcatttgc atctatagat gtaacaccag agttagatga aaatatagaa gtagaaatta
    39241 atccatcaga tttaaagata gacacatata gagcttctgg agctggaggg caacatgtaa
    39301 atacaactga ttcagcagtt agaataacac atatccctac tggtgtagta gttcagtgcc
    39361 aaaatgaaag gtctcagcat ctaaataagg acagagctat gagactttta atggcaaaat
    39421 tgatagaatt aaaagagtta gaacaaaagg aaaaaataga agatatacaa ggcaaatact
    39481 cacaaataac ttggggtagt caaattaggt catatgtttt tcaaccatat aaattggtta
    39541 aagaccatag aacaaatgct gagtttggaa atgtagatag tgtaatgaat gggaatatag
    39601 acttgtttat taatgaatac ttaaaaatga ataaaatagt ttaataaata tgcactgtaa
    39661 ttaagatata tgtataagaa ggttagtgct taatatgcac ttacagcact taggctgagg
    39721 acgataaccc tcagccttcg ttctgttttg ttatacttat gaaaaactaa cattaggagt
    39781 gaataaattg gatataaatc aaattttaaa aaaagagttt aatcttagag atgaacaaat
    39841 aaataataca ttaaaactta ttgatgaagg aaatacaata ccatttatag ctagatatag
    39901 aaaagaaatg actggcgaaa tgagtgatgt tacactaaga gaattttatg aaaagttaat
    39961 gtatttgaga aatcttcaaa gtagaaaaga tgatgtagtt agactaatag atgaacaagg
    40021 taaattaaca gatgaaataa cacaaaatat agaaaaagca aaaacactac aagaagtaga
    40081 agatatatat gctccatata aacaaaagaa aagaactaga gcaacaatag caaaagaaaa
    40141 aggattagaa aaattagcat tgtcaatact agagaataat ttagataata tagaaataga
    40201 ggcaaagaat tatcttgatg aagagaaaga agttctttct attgaagatg ctctaaaagg
    40261 tgctagagat ataattgcag aactagtctc agatgatgct aagattagaa agtatataag
    40321 agaattagct ttaagagaag gtatgatagt tagtaaaagt gccactgatg agaaatcggt
    40381 ttatgacatg tattatgatt atagtgaagc agtaaaaagt atgcctccac atagagtttt
    40441 ggcaataaac agaggagaaa aagaaaactt cctaaaggtt aagttagaaa taaataatga
    40501 taaagtttta aattacataa taaacgaata tgtaaatgat aaaaatttca aaaataaaga
    40561 agaaatcgta agttcaatag aagattctta taaaagatta attttcccat caatagaaag
    40621 agaaataaga aatcatttaa cagaaatagc tcaagaaaga gctataagtg tatttggaaa
    40681 gaatgttaaa agtttattgc ttcaaccacc agttaaagac aaagtagtta tgggatttga
    40741 ccctgcattt agaactggat gtaaaatagc tgtagtagat aaaaatggta aactactaga
    40801 ttatacaaca gtatatccaa cagaccctca aaatgatgta gagggagcaa agaaagtttt
    40861 aaaaggattg attgaaaaat acgatataga tataatttca attggaaatg gtactgcatc
    40921 tagagaatca gaaacttttg tatcagaaat gataaaagaa atagataacg aagtgcagta
    40981 tgtgatagtt agtgaagcag gagcatctgt ttattcagcc tctgaacttg caaatgagga
    41041 acatccagat ataaatgtat caataagagg agctatatct atagctagaa gattacaaga
    41101 cccattagca gagcttgtaa aaatagaccc taaaagtatt ggcgttggtc aatatcagca
    41161 tgatttaaac aaaaagagat tagaagaagt actagatgga gttgttgaag attctgtaaa
    41221 tagtgttgga gtagatttaa atactgcatc atactcccta ctagaacatg ttgcaggaat
    41281 aagtaaaact atagcaaaaa atataatagc atatagagaa gaaaatggag attttacgtc
    41341 aagagcacaa ttgaaaaaag taaagagact aggaccacaa gcatttactc aatgtgctgg
    41401 atttatgaga atcttagagg gtaaaaatcc actagataat acaggtgtgc atcctgaaag
    41461 ttatgatata tgtaaaaaaa tgattgagat aattggatat tctttagatg atgtaaagaa
    41521 taagaatata tgtgaaatag atgaaaaaat aaaagaaatt ggcttgagag aattaagtga
    41581 aaaactggaa gtagggcaag ttacgttaaa agatatcata actgaaatca aaaaaccagg
    41641 aagagaccca agagaagaag gtataaaacc tatacttaga actgatgtct taaaaattga
    41701 agatatacaa gagggtatga ctttaaaagg tacaataaga aatgttgtag attttggtgc
    41761 atttgtagat attggaataa aaaatgacgg attagttcat aaatcagaaa tgagtaatag
    41821 ttttgtgaaa gaccctatga gtattgttac agtaggtgat atagtagatg taaaagtcat
    41881 tggaattgac ttaaacaaaa aaagagttgc cttatcaatg aaaaaataga taagtttatg
    41941 tatggttata gtacttaaat tctatgtttg taaatgaggt tactcgagtg tataaatgtt
    42001 aaaaatttgt aaaagtatac agtaagaaaa tatattaaaa taagaacgat agtcttgaaa
    42061 atcaggagga aaatgaatga tttttataaa aaatggtaaa ataaatacca taacaaatgg
    42121 gataattcat ggagatatac ttatagatga aggaaaaata atagaaattg gagaagattt
    42181 gatagctcct ttagatgtag aggtaataga tgctagtaat aaacttgtgt ttcctggatt
    42241 tatagatgct catactcatt taggtttgtg ggaagatggt atagggtttg aaggagcaga
    42301 tggaaatgaa gagactgacc caataacacc acaattaaat cccatagatg gaattaatcc
    42361 aatggataga acttttaaag aagcttttga aggtggaata acttctgttt gtacaacccc
    42421 tggaagtgca aatgttatgg gtggtcaatg tatagctata aagacatgtg gaaaaagaat
    42481 tgataaaatg gtaataaaaa atccagttgc atctaaaatc gcttttggag aaaatccaaa
    42541 gagctgttat ggtcaggatg ataaatctcc tcaaactaga atggctatag ctgctttact
    42601 tagagaaaac ctaaaaaaag cagaagaata tttagaggat attgatatgt atgagagtca
    42661 tgatgatgaa gactgtgaaa aacctgaata tgatataaaa atggaaagtt taataccagt
    42721 tttaagaaga gaaattcctt ttaaggcaca tgctcataga gcagatgata tgtttacagc
    42781 aataagaatt gcaaaagaat ttaatttaaa gcttacatta gaccattgta cagaaggtca
    42841 tcttattgta gatgaattag tggaagaagg gtttcctgta atagtagggc catctttgtc
    42901 agaaaggtct aaatttgagc ttagaaattt gacatttaat acagcaggaa tactttcaaa
    42961 tgcaggtctt gatgtatgta taatgacaga ccatccagta ataccagttc aatatcttcc
    43021 aatatgtgca ggtattgcag tcaaacatgg tatgaaagaa gaaaaagcaa tagaatcaat
    43081 tacgataaat ccagctaaga ctttaggaat agaagataga gtaggctcta tagaagttgg
    43141 taaagatgca gacctagtaa tttgggataa ttctccacta gagattcaaa gtaatgtact
    43201 ttacactata ataaatggca aagtagtgta tgagaaaaaa taacttgaca attattaaaa
    43261 aattaactaa tataagagtg taaggaaaat taaaaaatca aagaagatct tcggggcagg
    43321 gcgaaattcc ctaccggcgg tatagcccgc gaactactta agtggttgat ttggtgagat
    43381 tccaaagccg acagtaaagt ctggatgaga gaagatattt atggaagata aataagttta
    43441 ttttagtttg tttaaactat atccataaaa actcgaagat agggtcttcg agttttttgt
    43501 ttttcctaaa aaatataaac atattgatta gggggaaata attatgcaaa acacagccaa
    43561 aagaggcaat atgatttcaa caagaacttt agtagtaatg tcaattttat ctgcaatatc
    43621 ttatttatta atgtttattc aagcaccaat accaggtata tttccagatt tcttaaaaat
    43681 tgacttatca gatattccag caatattcgg aggaatgtct ctaggaccat ttgttggatt
    43741 tggaatagtt gtagtaaaaa atgtgttaca agcaataaca gcaacaacaa ctggaggaat
    43801 aggagaattt gcaaatatat taattggtgg ttcttatgta atgatacttt gttattcata
    43861 taagagaagt ggagatttaa aaggtgtatt gattggtttt gtattaggaa caatatctat
    43921 gactataatt ggttcattag taaattattt tgtaatgttg ccactttatg gtcaacttat
    43981 gggactagat gctataatag gtttaggttc agctataaat cctcaagttc atgatttatt
    44041 cacttttgta atttggatga tagctccatt taatgtgctg aaggctgtta tactatcttt
    44101 agtaacattg ccactttata aaaaaatggg aaatgtaata aagaaataat tagacaagaa
    44161 aatataaaat taaatgtatt gaatagccat aatttattta taacttcagt cctatattgt
    44221 gattgatata ttaaatatat tatggcttta ttaatttatc aaaaaatgta tataaaataa
    44281 gaaatattta gacaaattta aattttaaaa taaaggaaga attaaaacga ttaactatta
    44341 aaatatg
//
LOCUS       DAMKMI010000016        43261 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMKMI010000016 DAMKMI010000000
VERSION     DAMKMI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.016"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            197..661
                     /locus_tag="PC429_000847"
     CDS             197..661
                     /locus_tag="PC429_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF3846365.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            668..1162
                     /locus_tag="PC429_000848"
     CDS             668..1162
                     /locus_tag="PC429_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF3846366.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            1178..2203
                     /locus_tag="PC429_000849"
     CDS             1178..2203
                     /locus_tag="PC429_000849"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF3846367.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            2190..4637
                     /locus_tag="PC429_000850"
     CDS             2190..4637
                     /locus_tag="PC429_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF3846368.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            4817..6190
                     /gene="radA"
                     /locus_tag="PC429_000851"
     CDS             4817..6190
                     /gene="radA"
                     /locus_tag="PC429_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF3846369.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            6193..7263
                     /gene="disA"
                     /locus_tag="PC429_000852"
     CDS             6193..7263
                     /gene="disA"
                     /locus_tag="PC429_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF3846370.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            7352..8446
                     /locus_tag="PC429_000853"
     CDS             7352..8446
                     /locus_tag="PC429_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF3846371.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            8575..10752
                     /locus_tag="PC429_000854"
     CDS             8575..10752
                     /locus_tag="PC429_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846372.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KETYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TIYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWYIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(10938..11783)
                     /locus_tag="PC429_000855"
     CDS             complement(10938..11783)
                     /locus_tag="PC429_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3846373.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            12000..13166
                     /locus_tag="PC429_000856"
     CDS             12000..13166
                     /locus_tag="PC429_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF3846374.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYVCGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            13188..14834
                     /locus_tag="PC429_000857"
     CDS             13188..14834
                     /locus_tag="PC429_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF3846375.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            14931..16304
                     /locus_tag="PC429_000858"
     CDS             14931..16304
                     /locus_tag="PC429_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3846376.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            16749..17702
                     /locus_tag="PC429_000859"
     CDS             16749..17702
                     /locus_tag="PC429_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF3846377.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            17740..18702
                     /locus_tag="PC429_000860"
     CDS             17740..18702
                     /locus_tag="PC429_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF3846378.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            18727..19713
                     /locus_tag="PC429_000861"
     CDS             18727..19713
                     /locus_tag="PC429_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF3846379.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            19726..20772
                     /locus_tag="PC429_000862"
     CDS             19726..20772
                     /locus_tag="PC429_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF3846380.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            20939..22669
                     /gene="lpdA"
                     /locus_tag="PC429_000863"
     CDS             20939..22669
                     /gene="lpdA"
                     /locus_tag="PC429_000863"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF3846381.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            23087..26035
                     /locus_tag="PC429_000864"
     CDS             23087..26035
                     /locus_tag="PC429_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3846382.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            26013..26477
                     /locus_tag="PC429_000865"
     CDS             26013..26477
                     /locus_tag="PC429_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846383.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            26590..26892
                     /locus_tag="PC429_000866"
     CDS             26590..26892
                     /locus_tag="PC429_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846384.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            26954..28318
                     /locus_tag="PC429_000867"
     CDS             26954..28318
                     /locus_tag="PC429_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF3846385.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFASVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            28353..28826
                     /locus_tag="PC429_000868"
     CDS             28353..28826
                     /locus_tag="PC429_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846386.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            28998..29648
                     /locus_tag="PC429_000869"
     CDS             28998..29648
                     /locus_tag="PC429_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF3846387.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            29822..30754
                     /locus_tag="PC429_000870"
     CDS             29822..30754
                     /locus_tag="PC429_000870"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF3846388.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            31028..31741
                     /gene="ispD"
                     /locus_tag="PC429_000871"
     CDS             31028..31741
                     /gene="ispD"
                     /locus_tag="PC429_000871"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF3846389.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            31738..32223
                     /locus_tag="PC429_000872"
     CDS             31738..32223
                     /locus_tag="PC429_000872"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF3846390.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    32318..32526
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            32590..34305
                     /locus_tag="PC429_000873"
     CDS             32590..34305
                     /locus_tag="PC429_000873"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF3846391.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            34427..35872
                     /locus_tag="PC429_000874"
     CDS             34427..35872
                     /locus_tag="PC429_000874"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF3846392.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            36312..37793
                     /locus_tag="PC429_000875"
     CDS             36312..37793
                     /locus_tag="PC429_000875"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF3846393.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            38014..38169
                     /locus_tag="PC429_000876"
     CDS             38014..38169
                     /locus_tag="PC429_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846394.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            38314..39711
                     /locus_tag="PC429_000877"
     CDS             38314..39711
                     /locus_tag="PC429_000877"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF3846395.1"
                     /translation="MKVYNALTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            39696..40109
                     /locus_tag="PC429_000878"
     CDS             39696..40109
                     /locus_tag="PC429_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF3846396.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            40215..40973
                     /locus_tag="PC429_000879"
     CDS             40215..40973
                     /locus_tag="PC429_000879"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF3846397.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            40986..41723
                     /gene="rlmB"
                     /locus_tag="PC429_000880"
     CDS             40986..41723
                     /gene="rlmB"
                     /locus_tag="PC429_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF3846398.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            41726..42253
                     /locus_tag="PC429_000881"
     CDS             41726..42253
                     /locus_tag="PC429_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF3846399.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            42318..42965
                     /gene="sigH"
                     /locus_tag="PC429_000882"
     CDS             42318..42965
                     /gene="sigH"
                     /locus_tag="PC429_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF3846400.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            43039..>43261
                     /gene="tuf"
                     /locus_tag="PC429_000883"
     CDS             43039..>43261
                     /gene="tuf"
                     /locus_tag="PC429_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF3846401.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETP"
BASE COUNT        17728 a         4481 c         8115 g        12937 t
ORIGIN      
        1 catgaagcaa aaaattagca tttggttatc aataaatata ataaataaaa actcataatt
       61 aatgaaagaa taaattcatg tctaaatttc tataaaaaat ttaaatttat acaatttagg
      121 gctttaaaaa tttaaataat aggtgtaaaa taatagtcaa agaaagtcaa aaataaaaag
      181 gtgtgtggtg atattaatgg caactatgac agatataatt gaaaaattta ttaaagattt
      241 gatggaagaa gataatagca tacaaattca aagaaatgaa ctagcaaact tgtttagttg
      301 tgcaccatca caaataaatt atgtcttaac aacaagattt acaatagata gaggatatta
      361 cattgaaagt aaaaaaggtg gcggaggata tgttcaaata gaaaaaatac gaaaaagtaa
      421 agatgggcat attagagaac tactaaatga aaaaatagga agtcagatat cctataaaaa
      481 agcaaaagaa ttattagaag gtttaaaaga gtcggatttg attaacgaaa gagaattaaa
      541 attaatatta tatgcaatag atgataaatc attgtgtatg ccaatatatg aattaaaaga
      601 gaaggtaagg tctaatattt taaagaatat aataatagga ttatttagta tagaggagtg
      661 ataatttatg ctatgtcaaa aatgcaataa gaataaagca tctgtatatt ataataagat
      721 agtcaatgga gaaaaaacag agatgtattt atgtagtgag tgtgcaaaag agaatacaga
      781 aatgaatttt aacttagata tgcctttttc aatgatggat atattttcta atttaggttt
      841 tcaacctaaa aaagaacttg aggaaaagct agtttgtcct aaatgcaata caacatatag
      901 tgagtttaag aataatggga gatttggatg tagtgaatgt tataatgcat ttagtagtca
      961 agtaaatcca atgcttcaaa atatacatgg gcatatagaa catacaggaa aagcacctaa
     1021 aaaatctttt tataagataa gtgtagaaaa tgagattaaa gaactaaaag aagatttaga
     1081 tagagctata aaaaatgaag agtatgaatt agcagctcaa tttagagata aaattaaata
     1141 tttaaaaggt tctattgact aagattggag tgatattatg aaagagaaca tagtcatgaa
     1201 atcaagagtt aggctagcta gaaatttaaa taactatcca tttccaaata aattagataa
     1261 agaatgtgcc atggaaataa tagaaaaagt aaaaaatgcg tttattaatt ctaacttaga
     1321 acaaaaagaa gaatttgatt tctataaaat tgaagattta gaccaatcta aaaaaatgct
     1381 tatggttgaa gaacatataa taagcccaga tttagcagaa aatgataaaa gtgcagttat
     1441 agtaaaaaaa gataaaacta taagtataat gataaatgaa gaagaccata taaggataca
     1501 aactatatgt gatgatttaa acttagaata tgcatatagt gtagcaaatg aaatagatga
     1561 tttgttagaa tcaagtttag aatatgcctt taacacaaag ctaggttatc taacatcttg
     1621 tccaactaat acaggaacag gaatgagagc ttctgtaatg atgcatttac cagcattaag
     1681 tcaactaggc tatatggatg aactttataa aatttcatca caaataggta tagcaattag
     1741 agggatatat ggagaacgaa cagaagcatt gggaaatata tatcaaatat ctaatcaact
     1801 tacacttgga agaacagagt ctaatattat agaaaatgta agtggactta caaaagatgc
     1861 aatttcaaaa gaaataaaag ctagagaaat attgcaaaaa aaattaggta taaaactaga
     1921 agataaaata tttagatcga tagggacact tgaaaattca agagtaatga gtagcgcaga
     1981 ggcaatgagc catctatcaa atataaaaat gggaatagaa atgaactata tagataaact
     2041 agatttaagg gctatagaac aacttatgat aggtatacaa ccagcgcatc agtcaataat
     2101 gtataagagt gatgatgttg agaatagaga tataaataga gcaacatata ttagagaaac
     2161 tttggaaaaa ttaagaggag ggatgaattg tgaacttcaa tagatttaca caaagagcta
     2221 aaaaggcaat tgatttagcg tttgaatctg ctaaaagtct aggacacaat attgtcggaa
     2281 gtgaacatat acttttaggt cttttaagag aagaagaagg tatagctgct aaagttctaa
     2341 gtaaagttgg atttacagaa gcttatttag aaggcaaaat agttgatatg gaaggtaagg
     2401 gagaagaaat ctcagaagat atagtattaa gtccaagaag taaacaaata cttgaattat
     2461 caggaatgtt tgcaaataaa ttaaaaacaa attatattgg gactgagcat atattattag
     2521 caattattca agaaggtgaa ggaatagcta ataagatttt aaattatgct ggagtaaatg
     2581 ataggacttt agcccaatta acaattgata tgatgggtat tagtgataaa aatcaataca
     2641 aagcagaaaa tagttacacg ggcaaccaga atcaagcaga atctaaagta cttgataagt
     2701 atggaagaaa tcttacacta tatgcaaaac agaataaaat agaccctgta ataggtagag
     2761 aaaaagaaat acaaagagta atacaaatat taagtagaag aactaaaaac aatccagtgt
     2821 tgataggaga cccaggtgta gggaaaacag ccatagctga aggtctggct acaaatatag
     2881 ccttaggaaa tgttccagaa acactaaaaa acaaaacttt atactcttta gagatgggtt
     2941 cattattggc tggggctaag tatagaggag aatttgaaga aagaattaaa gaagttgtag
     3001 atgaagttgt taaaaatgga aacataatct tatttataga tgagatgcac acaataatag
     3061 gggctgggtc tactggagaa ggctctatag atgcttcaaa tatattaaag ccagccttag
     3121 caagaggaga aatacaagtt ataggtgcaa ctacaattga tgaatataga aagcatgttg
     3181 aaaaagattc tgctctagaa agaagattcc aaccagttat ggtagatgaa ccaactaaag
     3241 aggactcaat aaaaatatta gaaggattaa gagataaata tgaagctcat cataaagtta
     3301 aaataactga tgatgctata aaggcagctg tagaattatc aactagatat atatcagaca
     3361 gatatctgcc agataaagct atagacttga ttgatgaagc tgcatcaaaa gtaagattaa
     3421 aagaaaatac acctccttca gaaataaaaa aattagaact agaaatagaa aatatagata
     3481 aagaaaaaga agaagcagta agatgtcaag actttgagaa agcagccaaa ataagagatg
     3541 aacaaggtct acttaaaaaa caattagaag atgttagaga aagatggaac aagtcatcta
     3601 aacattcaga tttagttgat ggagaagtta ttgctgaagt agtgggctta tggacaggaa
     3661 taccagttaa taaaattctt gaagaagagg ctgatagact tttgagactt gaggaaatac
     3721 tgcacaatag agttataggt caagaacaag cagtaaaatc tatttcaaag gcaatcagaa
     3781 ggtcaagagc aggtcttaaa gaccctaata gaccaattgg ttcattttta tttttaggtc
     3841 caacaggagt aggtaaaaca gaattatcta aggcattagc agaagtgcaa tttggagatg
     3901 aaaatcaaat aattagaata gatatgtctg aatatatgga aaaacatgct gtgtcaagaa
     3961 tgataggttc acctccaggt tatgtaggtc atgatgaagg tggtcaatta actgaaaaag
     4021 taagaagaaa tccatattca gttatattat ttgatgagat agaaaaagct catccagatg
     4081 tatttaatat tctgttacaa attctagatg atggtagact aactgattca aaaggaagaa
     4141 ctgtagattt taagaatact attgtaataa tgacatcaaa tgttggagca tctacaattg
     4201 gtagacaaaa aactttagga tttagtatag ctaaaggaga cgaagaagaa aaatctcaat
     4261 atgaaaaaat gaaagaaaat ataatgggtg aattaaaaca aagattcaga ccagagtttt
     4321 taaacagaat agatgatata attgttttcc actcactaaa tgaaaatcat atatcaaaaa
     4381 tagtactttt aatggcagcg aaactacaag agagattaaa agaaatggat ataaaattag
     4441 aaatgagtga tgaggctgtt aaattaattt ctaaatctgg atttgactta gaatatggag
     4501 caagacctct taaaagagct ttacaaaaag agttggaaga tgagctatct gaagcaatct
     4561 taaaaggtaa tgtgaaaaag ggaagtaatg tagtagcaaa agtcaaagat gaaaaaatag
     4621 tttttgaaac taaataattt aatagagttg cattttaaaa tattgcaaat ataagtttta
     4681 taatctaaac aaaattttaa gggcaatgaa tctataataa ttcattgtcc ttaattattt
     4741 tatttaatct ttacatattg tctttcagat atgctaaaat tatagtatag aataaaaaat
     4801 taaggatgat aaaagtatgg caaaaataaa aacaaaatat gtatgtcaat cttgcggata
     4861 tgaaacagct aaatggcttg gtaaatgtcc agaatgtaca aaatggaata catttgtaga
     4921 agaaatagac caaaaaagca ccaaaaaaga ggtctttata atagataaat cttcatctaa
     4981 accagtcagt ataaactcaa tagaaagtaa agaagaagag cggtttacaa cagatataaa
     5041 tgagttagat agagtgcttg gaggaggtat agttaaaggc tctttagtgc ttgttggtgg
     5101 tgaccctggt ataggtaaat caacactttt aatccaagta tctagtaatg ttgccaattt
     5161 aggaaaaact gttttatata taactggaga agaatcagaa tctcaaataa aaatgagagc
     5221 taaaagatta ggtattaact cagaaaattt atatatattt gctgaaaata atttaagtat
     5281 aattgaatct tatttagaaa gtgtaaatcc agaattaata attctagact caattcaaac
     5341 tgtcttcagt ccagaaatat cctcggcacc aggaactgtc agccaaatta aagaaggtac
     5401 ttcaaaattc atgaaaattt ctaaaaaaat gggaatttca acgttcatag ttgggcatgt
     5461 tacaaaagaa ggttcactag caggaccaaa attattagag catatggttg atactgttct
     5521 atattttgaa ggagaaagat ataatactta tagattagta agagctgtta aaaatcgatt
     5581 tggttcaact aatgaacttg gagtatttga aatgagggat ttaggattag tagaacttga
     5641 taatccatct aaaattttaa tctctgaaaa accaaaagat gtagcaggtt cagtaataat
     5701 atctactgta gaaggtacga gaccaatgtt acttgaattg caagcattag tttctcctac
     5761 aagttttggt ataccaaaaa gaacatctac aggagtcgat tacaatagag taggtatgtt
     5821 attagcagta ttagaaaaac gtgttggttt gcaaatacaa aatcaagatg tctatataaa
     5881 catagttgga ggaataaaga taaatgaacc ctcaattgat ttagggatag ccatttctgt
     5941 agcatctagt tttagaaata taccgataga tgaagatata gctgttacag gagaagtggg
     6001 acttacagga gaagttagag cagtaagctt tatagaaaag agaatagctg agtgtaaaaa
     6061 acttggattt aaaaagatag tagttcctag aagtaattat gatgttgtaa aagagaccaa
     6121 aggcatagaa atatggccag tagataactt aagacaagct ataaatatag tacttgggag
     6181 gaatcaataa atatggagaa ttttctagat aataaaaata tgctatatgc attaaaaatg
     6241 atatctcctg gaactccact tagattaggt ctaaacaatg tactaagagc taagactggt
     6301 ggattaattg taattgcaac aaacgaagat gtaatgaaaa tagtagatgg aggatttgct
     6361 ataaatgcag aatattcacc atcatatcta tatgaattag ctaaaatgga tggagctata
     6421 gttttaagtg gtgatgtaaa gaaaatatta tttgctaatg cacaacttat acctgactat
     6481 tttatagaaa catcagagac aggaacaaga catagaacag cagaaagagt agcaaaacaa
     6541 actggtgcta tagtcatagg aatttcacaa agaagaaatg ttataacagt ttatagagga
     6601 aatgagaagt atgtagtcga agatatatct aagatattta ctaaggcaaa tcaggctata
     6661 caaactctgg aaaaatataa gacagtattg gaccaagctg taacaaattt aaatgcctta
     6721 gagtttaatg atttggtaac tatttatgat gttgcattag tcatgcaaaa gatggaaatg
     6781 gtaatgagag ttacaagtat aattgaaaaa tatgtgatag aattgggtga tgaaggaact
     6841 ttagtaagta tgcaattaga agaattaatg ggtacaacca gaatagacca gaaattaata
     6901 ttcaaagatt ataataaaga aaacacagaa ataaaagaac ttatgaaaaa ggtcaaaaat
     6961 ttaaattcag aagaactaat agaattggtc aatatggcaa aactattagg gtatagtggt
     7021 ttttcagaaa gtatggatat gcctataaaa acaagaggtt ataggattct tagcaaaata
     7081 catagactac caacagcaat aatagaaaac ttagtaaatt attttgaaaa ctttcaacaa
     7141 attttagatg catctattga agaattagat gaggttgaag gaataggtga aataagagca
     7201 acatatataa aaaatggact cataaaaatg aaacaattag tcttattaga tagacacata
     7261 tgatgaattt taaatgaatt ttactacttt tttcttgaat ataatataca ctttggtcta
     7321 taatagataa gtataactag ggggtgaagc attgataagg aaagtaacaa gaatattgtt
     7381 tattttactt ggatttacaa taggtataac aacctattta acattaatga aagattttga
     7441 aatattaaca tttggaaaag aaacatatgg ttatatcgca gctgtagtag caggtataat
     7501 aatagccatt ttaggatatc ttatagaacc atgggttgta aataaagtaa aagaaattgc
     7561 taaaatagtg gataaagaat tgtcaaaata tcctcagact gatatattgt tagggtctat
     7621 gggtcttata gtaggatttg ttattgcgta tttattaagt ggattagtaa atagaatacc
     7681 aatagtaggt ggaatactat ccttactatt atatttattc ctaggatatc ttggaatgaa
     7741 agttgcgctt aaaagtaaaa atgatttatt taatattgga aaactaggaa gattagcaaa
     7801 tcctattaag gataaagata aggataaaga aaataaaaaa gaagtaaaag ccattcctcc
     7861 aaaagtatta gatacaagtg taataattga tggaagaata gctgatattt gtaagactgg
     7921 gtttatagaa ggaaaactta ttattcctgc ttttgttttg gaggaactaa gacatattgc
     7981 agattcttca gatgatttaa aaagagtaag aggtagaaga ggtctggata tattaaatat
     8041 aattcagaaa gaattaaata tagaagttga aataagtgag agagactttg atgatatagc
     8101 agaagttgat agtaaattat taaagcttgc tcaggtttta aatggaaaag ttgttacaaa
     8161 tgattataac ttaaataaag ttgctcaatt ccaaggtgtt gaagtattaa atataaatga
     8221 gttagctaat gctataaaac cagttgctat accaggtgaa gatatggttg ttcaagtggt
     8281 taaagaagga aaagaggctc aacaaggtgt tgcatatcta gatgatggaa ctatgatagt
     8341 tgttgatgga ggaagaaaat atatgaatga gactataaaa gtactcgtta cttctgttct
     8401 tcaaactcct gcaggtagaa tgatatttgc aaaacctaaa aattaatatg ttcaaagaaa
     8461 cctataattt gggctgttta ttttaaatag atggtccaaa tttatttatt tataacgata
     8521 atgtttaatg tattcttaaa gatggggatt taaatcagaa agcagggaga atttatgaga
     8581 aaaaagatta tatctttctt agcaacattt atcataatat taacatcagc aagtcagtat
     8641 agttttgcag atgatatatc aacacgtgga aaagtaatat ttatagatat gaatagaact
     8701 agtatgagta atatgttaag aataaaatca ttaagagaag aactagacaa tagagggtat
     8761 attggcttga tgaatataag aggcgataaa ggaagtgatg atagaaggtc atatgctagt
     8821 atgggtgctg gtggtagagc taatgtggct aatgaagaag atattaattt tgaaagctcg
     8881 agtaaggata gaaatatagt ttttgaatca gctacaggaa aaagtgcaaa aggtataaat
     8941 aatttaacta taaataagtc tataaatgaa aatttaaact ttggagagta tggttctgta
     9001 ttagggtcat taggacaaag tttatctgat aatggattaa aagcatctgt attgggtaat
     9061 tcagatataa ttgaaaatgg tcaacttata aaaaatagaa acttatgtct aactgcaatg
     9121 gatgaatatg gaagaatacc taatggaaat gtagatacta taaataaaaa agacttatct
     9181 atgccttatg gaatatcaac tgattatgat aaattgattg tagagactaa agaaacttac
     9241 aaaaataatg atgtgatttt tgtggagctt ggagacacat atagattaga tttatataaa
     9301 ccaaatttaa atgaaaaaac atatgagagt atgaaagata acatagaaaa aaatatagat
     9361 atatatttaa aaaatatatt ttctatggtt gaagaaaatg acactatcta catagcaagt
     9421 gcatttccaa gtgatttaga ttataaaaat aaaagaagac tttctccaat aataaaatta
     9481 aatggagaag ggaaaggtat attatcatct tcaactacta gaagagaagg tattgtaaca
     9541 aatttagatg ttggggttga aatattagac aactttaata taaaaaatca aaatatggtt
     9601 ggaagaaaat atgaacttat taacagagat gataataaag aatttttgat ggatgaatat
     9661 caaaagatag tatctatttc aagcataagg tctacaatac tgaatggttt tgtaagtata
     9721 gttttcttgt catggatagt tgctatgatt gcaattttat ttagaaagca tatatcaaaa
     9781 aattataaag aaacaacatt ttttatatta aaggaacttt taaaaatagg aatagtaatg
     9841 ccactgtcat ttatgataac accaataatg aatttcaaaa caccattagc aatatcttta
     9901 ggaataatta taataacact tactatatat cttataagta aagttttaat aaagaacgat
     9961 ttaaaaaaca tgctgttttt tactggatta accatagtaa ttatggttat tgatgctggg
    10021 tttggctcat atttaatgaa aagcaatgtt atgagttatg attgtataat tggagctaga
    10081 tattatggag taggaaatga gtatcaaggt gtggcaatcg gtagtgcaat atttacattt
    10141 gcaattttac ttacatataa aaatatacca aagtggtata taatagtttt ttctttggtt
    10201 atcttaatta caagtgcatc tccaattatg ggagcaaatg ttggaagtgc tatttctgaa
    10261 tgtgttgcat ttttactatt tatactattg atatataatg taaagataga tttcaaaaaa
    10321 atcgtattat taggtatagc agtagtattt gtattaggag tttttgtagc aatagatatg
    10381 attttgggaa gtaattcaca tttaggtatg tttgtaaaag aaatttactt taatggacca
    10441 ggagaaataa tacaaacatt tagtagaaag atagagatga atttaaagct agctcaaacc
    10501 agtgcatggg taaatatact gttaactgga ataggtgtaa ttttagttct tatgattaat
    10561 cagattagat actttaagca actgatggat gagtatccaa ttgtatttaa aggatttata
    10621 gcatcaatag caggatgttt agtaactctt ttagtgaatg actctgggat tgtgtcatca
    10681 gctacagcct ttatttatgt aattgtacct atgataacat taagtataaa tcttacagca
    10741 ttaaaagaat aagtactgta tgaataatat gtaaatatta atacattaat ttaacttctt
    10801 tatttacaaa ataaagaaga aagtttataa aaaataaatt ataataaaaa agagtttatc
    10861 tcaaaattga ttattctaat caaattttaa gataaacttt ttttattata aagataaata
    10921 acatataacc tcatatttta ggtagatttt tttcttatat gtttaggagt gcatttaaat
    10981 ttttccttaa aaattctgta aaataatttt gtattagtaa atccatgagc ttctaatata
    11041 ctgcataagg tatcatttgt gttaattaaa tctttataaa tatgagaaat acgcacatca
    11101 tttaaatact ctaagtaagt ttgtcccata tactgtttaa attttctaca aaaatattct
    11161 ggttgtaagt gtgctacctt agcgattttg ttaatagaaa taggcatttt gtaattaatc
    11221 tttgtatatt ctaaaactgg ttcaaatttt gacaaatcaa gtactgtttt tttcatattt
    11281 ttattaccta cagatatttt aaagtttgta tataattcaa ataaaagttc aaataataag
    11341 cttgtaaaga gcagattagc tgcaggagga gaactaagtt taatttctct catgttttct
    11401 aatataagtt taactttact tgaatttttt ttatagttat tgtcattggc attaacctta
    11461 aagtcaaaat agcaattttt aaaatcaggg atatatttgt ttagaaaaga caatggtact
    11521 tgcaataata tagaggtatt ttgataaata catctagttg agtgaataat tccagaatta
    11581 atgaacatac actcattagc ttttaaatta taagtgtaac cctccatgtt aatttgtaaa
    11641 tcaccagaag ttatataaat tatttctaat gagttatgcc aatgatttgc tacataacta
    11701 ccactatcat caaatagttt gaaattaaca tctaaattat caataggttc aattatttca
    11761 tgtgaaaaat tatttctgtt cataataata tcctccttat aattttaaat tataaatagt
    11821 aaatttataa tgttcaatat taaaaagata atatagacta ataaattaga taataaacat
    11881 acttaagaaa acggataaaa tcaaatataa taactgtata ttaaaataac ttaaaaaaat
    11941 tattttatta taagtgcaaa atatacctat ttaaataaga taagagggga ggattttatg
    12001 tgtatataaa aggtgtaaat ttaggaggtt ggttagtttt agaaaaatgg atgacctcaa
    12061 gtttatttga aggcacagaa gctgaagatg aatactattt accaagacag ctttcaagag
    12121 aggcatatga atctagaatt aagactcatc gtagtgaata catcacagag agagattttg
    12181 caacgattaa atctatggga tttaattcag ttcgtatacc agtaccatac tttatttttg
    12241 gagattgtga accattcata ggatgtgtta aagaacttga taaagcattt gcttgggcag
    12301 ataagtatgg gcttagtata ttgatagact tacatacagt tccaggaagc caaaatggat
    12361 ttgataatgg aggaatatca ggtatatgta gctggtcaca aaatccagaa tatgtagcgt
    12421 ttacattaaa tgtattagaa agacttgcaa aacgttatgg gatgaggcat gaattgtatg
    12481 gaatccaaat tttaaatgag cctataacag aaagaatgtg gaacataatg aatgttccaa
    12541 atcggtttaa ggctgtagat aaggagatgg caagggggag taaacctaat tcattagagt
    12601 ttttacgtga tttttatata aaagcatata gggtaatgcg tccttatatg agagaggaaa
    12661 acgttatagt gtttcatgat gcatttgaat taaaagcatg gaaagacttt atgagagagg
    12721 aagaatttaa aaatgttgtt ctagatacac accaatattt gatgttagct gaagcagatg
    12781 gatgtgaaca aagtattgat agctatctca aatatattag agaaaattat gctaaagata
    12841 ttttacaaat gcaaaaatat tttcctgtta tatgtgggga gtggagctta tttaattcat
    12901 atgtttgtgg tatagataca aatggaggtc aatcacctct aaatgggatt gaatcaaata
    12961 tagataaatt atcaaaagac gataaaagag aattatatag gaagatagca aaagcacagc
    13021 ttgatgcatg gagaaatgga agtggtcact actattggaa ttataagtta ttactagata
    13081 cagttaatga agaaggttgg ataggatggg atagttggga cctaggcaaa tgtgtagctc
    13141 aagaatggta tcctatagaa tattaaacta aaattggagg aaaagatatg attaaaaacc
    13201 ctatacttcc aggttttaat ccagatcctt gtatttgtag aaagggagat gattattatc
    13261 ttgtagtttc atcttttgaa tggtttccag gtatacctgt ttatcactct aaagacttaa
    13321 aaaattggga gttatatact catattttga cagatgaaac aaaaatagat ttgaagaagc
    13381 ttccttcatc taaaggaatt tgggcaccct gtttgactta ttgtgaagaa gaagatttat
    13441 tttacatagt ttatggaatt atgaactcta tgaatgcaag atattttgat gttgataatt
    13501 atttaattac atcaaaagat ataaagggag agtggagtga accagtatat ctacactcat
    13561 caggttttga tgcgtctatt tttcatgatg atgatggtaa aaagtggatt gcatcattag
    13621 actgggagac tagagaagga tatgaaaaac ctggtgttat atgtttagtg gaatactgta
    13681 caaaaaagaa agaaatagta ggatatccaa agagaatatg gtcaggggga actgatagag
    13741 gttgtattga agcaccacat ataacaaagc gtggagatta ctattatata atgtgtgcag
    13801 aaggtggaac tggatatggt catagtgtaa ctatgggaag ggctaaaaat atatggggac
    13861 cttatgaaaa agattcaatg aatccaattg taacttcaat tccaggcgat ttttatgaaa
    13921 gacatgaccc tgaccatcta aagcctaaat actataatcc agaatcaaaa cttcaaaaat
    13981 ctggtcatgg aagctatata gaaacaacat caggtgaagt atatttagtt catctaacat
    14041 ctagaccatt tgtaccagag ttaaggtgta ctttaggtag agaaacagct attcaaaaaa
    14101 tgaaatggac aaaggataat tggcttcgaa tggaagatga aagtaatttg gcaaaagaat
    14161 acgtatcaga aagtaaacta gaagaacatt tagtttcatc tataccaagt tttgatgact
    14221 ttgattcaaa tgaattaggt ttacagtact atgctcctag gatttcacca ttatcatttg
    14281 cagatgttaa gtctagacca ggatatgtaa ggattagagg acaagaatca agaacatcat
    14341 taaataaagt aagcatattg gcaagaaaat taacttcagt atatgcaaga ataaccacta
    14401 agatggagtt ttacccagag gtacatcagc atagtgcggg tttgataatg tattatgata
    14461 atatgaatta tataaattta agaaagtatt atagtgaaac attgggtcaa agtgcactat
    14521 caataataca tcttgaaaat ggagagaaaa cggaattttt aaatactagg ataccaataa
    14581 aagatatacc aatatatctt cgtctgtata tacaaggtcg taagtcatat tttgaatgga
    14641 gttatgatga aaaaaactac cagagaatag gaaaggtatt tgatacaact aagttttcag
    14701 atgagtactg taaatatgga gaatttacag gtacgtttat aggattaact tgtgcagatc
    14761 gtgtaaagca taaacactat gctgattttg atttttttga atatatagta gatgaatcaa
    14821 aagatgtcga ttaataaaat cattactaaa attttcagag caaaggtaaa tcttatgctc
    14881 tgaaataaga aacattagaa ggcaaatctt acaaaaagga gtatggtatt atgaatacag
    14941 aagttaatgt taaagcaact acgattagat atgaaaagat ttcatttttt gaaagaattt
    15001 cttatggttg tggggatttg gggtgcaata tcatatactc agctatgtca gcatttttgc
    15061 tgttttatta tactaattat gcagatgtta gtgcagcagc tgtaggtagt attatgcttg
    15121 tatctaggat actggatggg tttagtgact taacaatggg aattatagtg gatagaacta
    15181 aatccaaata cggtaaggct aggccttgga ttttgagaat ggcgattcct tttgctatag
    15241 cagcagtatt attattttct gttccatcaa atcttggtat cacttctaaa ttaatttata
    15301 tttttattac atataactta gtttctactg ttatttatac agcaatcaat gtaccctatg
    15361 caactctaaa ttcacttata actcaaaacc agtatgaaag aggagtatta agtatattta
    15421 gaatgatatt agccacttgt ggtaccttac taataaatgg acttacacta cctctagttg
    15481 aatactttgg aaataattta tcagcatgga ctaaaacatt ctttgtattt ggaatagcat
    15541 ctataatggt attttttata acatttactg gaaccaagga aagagtaaaa gcagttaagc
    15601 aagataggaa tgaagtaata ccatttaaaa taggtattaa atctttattt agaaataagt
    15661 actggataca aataacatta tgtttagttt gcatatttat tgtatttgcg ataaatggtg
    15721 gttcatccgt atattatgca aagtttattt tgggagatga aaaattattt ggtcctataa
    15781 atatggtttc aaatatatcc caaattatag ctatgtttat ggttgctcct tttataaaaa
    15841 aatttggaaa aagaaatgta ttaatagtag gttcaataat attaatatcg tcaaatgtta
    15901 tgtttataat agttggacaa aatcatatag gagtaatatt tgcaagtgct ataaagggaa
    15961 taggaagtgc tggaatagca tcaacaatgt ttgctattgt ttcagatact attgaatatg
    16021 gagaatggaa gactggatat agaactgagg ggcttataaa tagtgcatct agttttgggt
    16081 ttaaagtggg aaatggatta ggttcagcaa tattaggtgc agtattatca attggtggtt
    16141 atgtaggaac atcagctact caaagtgatt tagctatagt atcaataaaa gtatgtttta
    16201 tatacctacc tatatttata acaatattac aaattatcat aatgtctttt tataagcttg
    16261 ataaagaata tagtactata cttaatgaat taaatactaa ataatgaaaa agtttcttat
    16321 aaagtcttaa gtttaattaa ggtctattgt aaatgttccc tataaaaact attatatatt
    16381 tatgattatt gaagaatttt tagagaagcg aataataaaa atataagaag actttaaatg
    16441 atgaaatggt tggtttttta gaaatgattt aataagttat tgttatttaa agcattataa
    16501 gtggacaatt aatttttaat ttatggagat gatatttaac aattagaagt aatatataat
    16561 atgcactaag aagcaatttt tcaactattt atgaaggtgg tggtacaaga aaagtatcac
    16621 cttttttttg ttatacaaaa aaacagttga ctttttatat gaaccccttc tataatgtta
    16681 ctgaaattaa gtaaaatgat tgaacaaatg taaagataat tgaaaagctt gattttggag
    16741 gaaagcaaat gaaaaaaatt ggagacttaa gattaatgat gaagtgttgt tcactttatt
    16801 acgaagataa tttgaatcaa caggaaatag caaatcaatt gggaatatca agaccaacaa
    16861 tatcaagaat attaaaagaa gcttttgaac aaggaatagt aaaaatacag atagttgatg
    16921 ttttgaaaaa tgattaccaa aaaatagaga gaagtttgga gagaaaatat aaattaaaag
    16981 aagtaatagt agtagatgat aaacaggatg cactaacaca aaaacaagaa ctagcaagag
    17041 ctgtatctga atatttaact agagtagtaa aggaaaatga tataataggt gtttcaatag
    17101 gaacaacact aaaagaaata ccaagatatg tggaaaaaag taactgtaaa aatgttacat
    17161 ttatacctct attaggaggg attggagaca atgaaataga tattcatgca aatcaaatag
    17221 ccgtaagttt agcgagggct tttggaggtg attttaaatt attacatgct ccagctgtta
    17281 tgtctgactt gtctacaaaa gagaaacttt gcaaagacga aaaaattaaa gaagtactag
    17341 atttaataga taaaacaact atagcaattg tgggtatagg aaatcctatg agtttaaact
    17401 caacaataat ggcttctgga tacatgaatg aagcagatat agaagattta aaaaaataca
    17461 actcaatagg tgcaatttgt ttacaagcct ttgataaaga aggaaaaact tctatacttg
    17521 aatttaatca aagagtatta ggagtgaaac tggaagattt aaaaaagata aaaagaacaa
    17581 taggtgtcgc ttcaggagat gaaaaaatag aggcaataaa ggcatcatta aaagcgaaat
    17641 ttataaatag tttagcaatt aatcacagtt tagcacttaa attattagaa gattgtgact
    17701 aatagaaaga tagataaaag ttaacaggag ggaatgtata tgtcaatatc aaaagaaaca
    17761 ttgcttgaaa tgtataagag aatgaatcag gctagaaaat ttgaagagaa agttagttgg
    17821 ttttttgcaa gaggtatggt tcatggaaca actcacttat ctgtaggtca agaagcgtca
    17881 agtgtggcgg ctgtaatggc tttagaaaaa ggagatttag tatcattaac tcatagagga
    17941 catagtcaat ttataggaat gggaatagac ctaaataaaa tgatggctga acttatgggt
    18001 aaagaaactg gattttgtaa gggaaaagga ggctcaatgc atatagcaga tattgagtca
    18061 ggtaacttag gagcaaatgg agttgtaggt ggaggtctta caatagctcc aggagctgca
    18121 cttacacaac agtataaaaa aacaggcaaa atagttttat gtagttttgg tgatggagca
    18181 tcaaatgaag gtacttttca tgaaggaata aatttatctt caatatggaa attgccaata
    18241 atattttact gtgagaataa tttatacggt atgtcaacaa gcataaaacg tcatatgaat
    18301 atagaaagta ttgccactag agcagcttct tatggaatag aaggaatatc aatagatgga
    18361 tataacccaa tagaagtata cgaaacagta caaaaagctg ctgagaagtg tagaagagga
    18421 gaaggacctg tattaattga aagtagaact tatagatggt taggtcattc aaaatcagat
    18481 gcaaatgtat atagaacaaa agaagaaata gaatcatgga aagcaaaaga ccctattgaa
    18541 tttttaaaaa attatcttat agaaaataat ctatctaatg aagatgaatt agataagatt
    18601 caagaatttg caaagcaatc aatagaagat gcagtagagt ttgctcaaaa tagcccaaat
    18661 ccaaaaatag agtctttatt agaggatgta tatgcagact aagaatggta aaggaggaaa
    18721 ataaaaatga gtacaaggga attaacatat gcacaagcaa taaaggaagc aatgtcagaa
    18781 gaaatgagaa gagatgaaaa tgtaatattt atgggagaag atataggaat atatggagga
    18841 gcatttggtg tatcagttgg tatgatagat gaatttggac cagaaagagt aagggatact
    18901 cctatatcag aagcagctat agcaggagcg gcagcaggtg cagcagcaac tggcttaaga
    18961 cctattatgg aagtaatgtt tatggacttt gtaacaattt ctatggatgc aattgtaaat
    19021 caagcggcaa aaatgagata tatgtttgga ggaaaagcac aagttccaat ggttgttaga
    19081 tgcccaggag gttctggaac tggttcagca gagcaacatt cacaaagttt agaagcttgg
    19141 ttttgtcatg taccaggagt aaaagtagtt gcaccttcta caccagcaga tgctaaggga
    19201 ttgttgaaag cagctataag agataataat ccagtcatat ttgttgagaa taaattgtta
    19261 tacagaaaaa aaggttttgt gccagaagat gattatgtaa ttgaaattgg aaaggcagat
    19321 attaaaagag agggtactga tgttacagta attacttatg gaagaatgtt acaaagtgtt
    19381 gaagaagcag cagagactct atcaaaagaa aatataaatg ttgaaattat agatttaaga
    19441 actttatatc cattagataa agagactata gtaaagagtg tatgtaaaac tgggagagtt
    19501 ttaatttgcc atgaagcagc taagactggt gggctaggtg gagaaatatc agccctgata
    19561 acagaaagtg aatcatttga ttatttagat gctccagtaa aaagaatttg tggaaaagat
    19621 gtgccaatac catataatcc agagttagaa aaagcagttg tcccaagagt tgatgaaata
    19681 gaagaagcaa taaaatcttt aattgttaga taaggaggga tagatatggt ggcaaataaa
    19741 ataaaagcca caccagcagc tagaagtcag gcaagaaaag acaatataaa attagatagg
    19801 ctgattggaa gtggagaaaa tggaaggatt catttagtag atgtgttaaa ttatttaaaa
    19861 gataataaag caaatactac tcctttagct agaagaatag cagaagattt aaatatagat
    19921 ttagaaacta ttgtaggtac tggatataat ggaaagataa gaaagtgtga tgtggaaaaa
    19981 ttaactggaa aagaaactat tgtatcaact aatacatcta aatctagtga gaaaaaagag
    20041 ctgaaaattg aaaatgaaaa ttcaagaatg tttaacacag tagaaggaat atttgaaaag
    20101 ccaaatccaa tgagagctac tgtagcaaaa cgaatgtcag aaagttattt tagtgctcct
    20161 gtatttacat tcaatataga agtagatgct acagaattga aggtacttag agcaaagtta
    20221 atcgacacag taaaagaatc aactggagta aaattaacta tgacagattt aatagtaatg
    20281 gcagtatcta aaatacttcc aaatcatcaa gccttaaatt cagcatggac agatgagggt
    20341 atatttagat ataaagatgt aaatatagct attgcagttg gtttagatga aggcttatat
    20401 gttccggtag taaaaaatgc aaacaaaaag tctttaaaag aaatagcaaa agaaagcaaa
    20461 gaacttgcgg aaaaagtaaa gacagggaaa cttatgccag cagaccaaga aggtaataca
    20521 tttacaataa gtaatgttgg aatgtatggt ataactacat ttacacctat aataaatatg
    20581 ccttcaagtg caatcttagg ggtaggtgct acacaagata agtttgttcc agtaaatgga
    20641 gaggctaaaa taaaacctat aatgaactta tcattaacat cagaccatag agtaatagat
    20701 ggaacagtag ctgcaaaatt cttaaaagat ttaaaagaat tgttagaaaa tcctttatca
    20761 atgcttgtat aggttttgat attttgttac tgatataaat agtttgaata gattatatta
    20821 ttataattgt ttcagtaaat ttaaagcttt tatatattaa gaattatttt taacagtaac
    20881 tatatgtgaa tatgatttgc ttaattaaaa tatatgaaaa taaataggag gagagcctat
    20941 gtcagtagaa gtaataatgc caaaagcagg agtagccatg gaagaaggta ctatagtatc
    21001 ttggctaaag caagaaggcg aagaagtaaa gattggagaa cctatactag aaataacaac
    21061 agataaagtt aatatggaga tagagtcaga aggagaaggt acacttgctg taatcataca
    21121 taaagaggaa ggtgaagtat taccagtatt tacagtaata ggtgtaatag cagaaaaagg
    21181 ggaaaatcaa gaggaagtaa aagctaaata tttatctgga aatgtttcaa aagaagatat
    21241 agttgaagag aatcaaaata tagaatttaa agaagaaaaa ataaacaaaa aagagtgtaa
    21301 tcatgattat gatgtagtgg taataggagg aggacctggg ggatatttat cagcattaaa
    21361 agcagctctt ttaggtggaa gagttgcttt agttgaagaa aatatactag gaggtacttg
    21421 cttaaataga ggttgtatcc ctacaaagac ttatataaaa acagcagaaa tattagaaga
    21481 aattgaccaa ttatcaaaga gaggtgtaaa agtaactgta gataaagaac aggacattaa
    21541 gaaggccata aaatataaaa atagagtagt taaaaaactt acagctggtg ttggcggatt
    21601 attaaaaagt agagatgtag atgtgtttaa tcttaaagct agtgttaaag aagagcataa
    21661 agtaatttta tcagatggaa aagttttaga tacagaaaat ataataattg ctacaggttc
    21721 caaagtaagg atattaccta taaaaggaat agaatcaaat ttaataataa ctagtacaga
    21781 agcattagat ttggaaacag tgccagaaga attagtaata attggtggtg gagtgattgg
    21841 ttgtgaattt gcagagatat tcaattcaag aggttctaaa gtaactatag tagaaatgga
    21901 agatagagta atcccaagaa tggataaaga gttaagcgaa tctttaaaat attctcttag
    21961 taaaaaaggt ataaatgtat taactaaaaa gaaagtttct gaatttaaag aagaaggaaa
    22021 taacatatta gtgtgcattg aaggtgaaga gccaataaaa gcagatttat gtctatatgc
    22081 tataggaaga gaagctaatc tttctggcat agaggactta gatattaaaa tagataaagg
    22141 ttcaatagtt gtaaatagta aaatggaaac aagtatacca agcatatatg cagtaggtga
    22201 tgttactgga ggtgtaatgt tagctcatgc tgcttttaaa atgggagagg tagcagcttc
    22261 aaatgctcta ggtgtgaata aagaagttga cttaggtgca ttacctagtt gtgtttatac
    22321 aattccagaa gtagcatcag ttggtattac agaagaggat gctagaaaaa aatataatgt
    22381 aaaagttggt aagtttaatt ttgctggaaa tggaagagct ttagcttctg gtcaagaaca
    22441 aggatatgtg aaggtagtag cagatgctaa atatggagaa attttaggta tacatatgtt
    22501 tggatgtggt gtagcagaac ttataaatca tgcagcatca tttaaagcgt tagagatacc
    22561 tactgatgaa gcaagtgagt tgatatttgg tcatccatgt acttcagaag ctttaatgga
    22621 agctttggca gatgtaaatg gagaatgttt acacttacct aaaaaataaa tctaaacata
    22681 aattattaat ttaagtctat tattaatatt aatttatatc ataaaatttt aaaacactta
    22741 cttagccata ccccttatta tgcaaataat ttatttgcat aataaggggt attttttatg
    22801 gaaaaatata gaatatagaa tccttgtagt ttttgttgta caaaaaagat agtaatataa
    22861 aataacatta tcattttaga atgatttcaa ttgtaattag gaaaagtgat ttttttgttt
    22921 ttaaaaaaag tattatttgt aaaatataaa aaaaaatttc ttaatatttg aataaaagaa
    22981 tagcttaatt taaatgttat tactttataa aaaaataata agtacaaaaa aaacaatata
    23041 tggcatataa gttgctatat aaataataag gagggaacaa ttataaatga ggcaaagaat
    23101 aaaaaactta attgaaaatg aagataagaa aaatccttta acagatgaag cgatttcaag
    23161 taggttaaat attcgaagag aagatgttac ctttttgaga aatgagttaa aaatagaaga
    23221 ttcaagacaa agaagaaaag ttgttttaat taaagctata aaagatattt taaaagaaga
    23281 aaaaactata aataaaaatg atataacaag aagattaaac agcattgggt ttacagtatc
    23341 aagatttaca gtcatacaat acttaaaaga gattcaagat tctggtgacc taaaacttga
    23401 taaagagagt gatgataaaa aagaagaaat aatcaataat agagaaaata aagatatagc
    23461 atttgataaa cttataggta gtagtggtag cttaagtaca attataaaac tagccaaagc
    23521 agcaattctt tatccacctc atggtcttca tactattatt atggggccaa ctggtgttgg
    23581 aaagagtgag ttagcagagt gtatgtataa atttgcagta gaatcatcca gattccctaa
    23641 gaatgcacca tttatagttt ttaatgcagc agattattca gagaatccac agcttttgct
    23701 atcacagtta tttggacacg ttaaaggtgc ttttacagga gctgatgaga acaaagaagg
    23761 attagtatca aaggcagatg gaggaatatt gtttatagat gaaatacata gattacctca
    23821 tgaaggtcaa gaaatactat ttcaactgat tgataaaggt atggtcagaa agttaggaga
    23881 aacaagacta acacataaaa tagatgtaat gattatttca gcaacttctg agccaataga
    23941 ttctcattta cttaatacat ttaaaagacg tatacctgta acaattgaaa taccagaatt
    24001 agttgcaaga ccattaaatg agaggtttga cattataaat aatttcttct taatagaagc
    24061 tcaaagaatg ggagcaaaca tacatataaa gtcagatgta ttaaaagctc ttatgcttta
    24121 cgattgtata ggtaatgtag gtcaacttag aagtgatatt caagtagctt gtgctagggg
    24181 actattaaac caacttacaa acaaactaaa agaagtaaat ataagcatat cagatgtacc
    24241 tggatatgta aaacaaggtc ttataaaaat cagaaattgt agagggaaaa tagaaaatta
    24301 tgtagatggg gatttaatta tagactcaaa tcttcaaggt gagatagaaa agaaaaatga
    24361 agatatatat acttttccag atgaaatata caaacttact gaaagaagac atattgattt
    24421 attggaacaa ggtttagatt ttgatgttat aaatagaatc attggaggag aattagaaag
    24481 ttgcattcaa aaatacataa aacaagttaa aaaagtttct attagtaaca atcttccaga
    24541 tataagtcca attgttggaa aatatatcgt agagctaact tatgaagtaa tcaaaatagc
    24601 atcacaatac ctaagttcac tagaaccgaa tctacattta tgtttagcag ttcacctaaa
    24661 agcagctttt gagagattaa aagaaggaaa agtaatatca aatgctcatt tgaaagaaat
    24721 aataggaaat tatccagtag aatattctgt agcagaaaag gtagctcaat atttaaagga
    24781 aagatataac ataaacttac caaaagaaga aataggtata atagcaatat attttaaaat
    24841 gagtagtaga agagatatgt ctgaacaaaa aaagataggc gtacttctta tggctcacgg
    24901 aagagtagca tcttctgtat gtgaggttac aaataaacta ttaggaatta atcacgcaag
    24961 gtatttagat atgccatttg agaagaagcc ggaagaaatg attgaagaag ctactacaat
    25021 aataaaagat attgatgaag gtagaggtgt gattatatta gtagatatgg gttcactttc
    25081 atattttgga gatattattt ctggaaagac tggaattaat ataaggacaa tatctagatt
    25141 tgatacattg ctttctatag aggttatacg aagagcagtt ttaccagatt taacaataga
    25201 cgagatagtt gatgaagtta aaggtataga gccacaagta aaagaaaagg ttagtaaatg
    25261 taacaataca aaagtagatt ttagaaaacc tataatttta acattatgta ttactggatt
    25321 agggggagct tgtaagataa aaaaaatctt agaggaaaat attaaaagta taactgaaaa
    25381 ggttgatata gaacctatag gtatgattga atatccaagt cttgatttag agataaaaca
    25441 aataagcaat gataaaaatg taatcgccat agttggaaca attgaccctc agatagcaaa
    25501 tatccctttt tttagtctag tagacataaa tgataaagcg aagttaaatc aaataaataa
    25561 cctaattgat acaaatcaaa ttggtgaaac aaataaaaaa tatgaattat cagatttaat
    25621 acataaagaa gatatatttg tggatttaca ggttaaatca aaagaagaag ttctaaaata
    25681 tatgagtaaa aaacttagag agaaaggtta tgtgacaaaa aatttctata aaaaagtagt
    25741 ggaaagagat aacttatttc caactgaatt atcagatgaa atagcaatac ctcacacaga
    25801 tggaatagat gtaattaggc cagctataag tatagccatc ttaaagaagc ctattgtgtg
    25861 gagtaaaagt aaagtaaaaa taatactttt attagctgta gaccaaaagt gttttaatcc
    25921 attaagcact ctactctcat ttatagaaac agatgaattt aagaagataa aaaacattaa
    25981 agatagagat tttattaggg agatgatatt agatggagtc aatgaaaata attagtagca
    26041 atttaatttt taaaaatatt gaagtgtcta acaatgaaga tgcattaaag tttttgggac
    26101 aacgtttgtt tgacgaacag tatgtaaaag aaagttacat acaagctgtt gtagcaagag
    26161 aaaaaaagta tgctacaggg ttaccaacag aaatatacgg agtggcaata ccacatacag
    26221 atatagtaca tgtaaatgag ccaggaatag caattggaat actgaataaa cctgtaaaat
    26281 ttataatgat gggtacagac gatactgaaa ttgatgtaaa agttgtattt atgttagcag
    26341 tgaaagagcc acaagaacaa ttgcaactat tagaaaaact tatgactata tttcaagata
    26401 agaatatact aaataatatt gttgacttaa gtgaagaaag tgtgagtgat ttattaaact
    26461 ctaaattgaa aaattaaaca tgtaaagtat attaaaaatg taaaattaaa catgcaaagt
    26521 atgttaaaaa atataaaaag attattaaag atttctaact atagatatct ttaaaataaa
    26581 ataatttcaa tgggggtgac taatgtggct aaaaagatat tagttgcatg tggaacagga
    26641 gtatgtactt caacaattgc tataaacaaa ttaaagaaag cacttcaaga cattggtaaa
    26701 ttggatatgg ttaatattac acaatgtaaa gtagtagaag ttgcatcaaa agcacctgat
    26761 tatgatttaa taatttgtac tacacaagta tctagtagta taaaaacacc agtgataaat
    26821 ggattacctt ttttaactgg tgtcggaatg gataaattaa tcaatgatgt attagaagag
    26881 ttagaattat aattaaaaaa attaaggaac atggtctcta aaatcataag ttttagagac
    26941 ttaaaattaa ataatggagg gtctttatat ggagatatta aatttcattg ttggtctagg
    27001 tgcacaagta atgatgccaa taataatatg tatttttggt ttgatattag gtacaaaact
    27061 aggaaaatct ttaagagctg gtttaactgt gggagttggg tttataggtc ttaacactat
    27121 tatagctttg ctaacagata atttaggtcc agctacacaa caaatggtta agaatttagg
    27181 gctaagttta tcaataatag atgtaggatg gccagcagca tcagctatag cttttgcatc
    27241 aacagtaggg gctttaatta ttccaatagg tcttgtggta aatatagtta tgctaattac
    27301 taatactact caaactgttg atgtagacat atgggattac tggcattttg cttttacagg
    27361 agctttagtg gcaggtgcta ctcaaagtgt aatgtgggga gtatttgcat cagtagcaaa
    27421 tatggtaatt gtattggtaa tggcagattt aactgctcca ggtatagagg aatatttagg
    27481 tatgccaggt atatctcttc ctcatggatt tacacaggca tttgttccaa ttgctatagt
    27541 agtaaataaa ttattagacc taataccagg tataaataag attgagataa atgctgatac
    27601 attgcagaaa aaatttggat tatttggaga acctttaata atggggtctg taataggtgt
    27661 aataattggt attgctgcga aatatgatat aaaaggaata ttacaattag gtgtaactat
    27721 gggagctgta ttaatattaa ttccaaagat ggcagcatta ttaatggaag gattattacc
    27781 agtatcagaa gctgcacagg aatttattga gaagagattt aaaaacagag gcaagattta
    27841 tataggctta gattcagcag taggaatagg tcatcctgtt acattatcag ttgcactagt
    27901 gttggttcct ttgactatac ttatagcagc tatattgcca ggaaataaag ttttaccatt
    27961 tgcagattta gcagtaattc catttgcact tgtattaatc gtaccaatta caaagggaaa
    28021 tgtgtttaga acattaataa taggaataat tataattact tctggattat taatcgcaac
    28081 taacttagct ccattattta cccaaatggc tttaaatgct agttttaaaa tgcctgaagg
    28141 agcaactatg atttcaagta tttgtgatgg tgcgaatcca ttaagttggg tatttgtaaa
    28201 agttatgaac tataaagtta ttggaggagt agtttttgga gttatagctt taggaatggc
    28261 tatttacaat aggaatagaa taataaatga aaataaaaag ctatctatag aagaataagg
    28321 ttaaaacaat tttattaagg agtgtatagg ttatgaaaaa atataaatat gattctaaaa
    28381 gatacttact cagtgttaca ataccaggat tttttatgat tttaatattg atttatgcac
    28441 tgtttaataa ctatacaaat tttaatttga atatatatag tttgttaata attgtctgta
    28501 cttatggtat attcaatact tttatatcat caagtaatcc taaaaaaatt attattgata
    28561 gcaaatgtat acattttacc tcatttatga ctacacataa gtatgaaatt aaagatatag
    28621 aaaagataag aataagagaa ttttataata aaaaaattta tgtaaaaatt aatgatggaa
    28681 acctttttaa aggtaggtat tggataagaa caaatatgtt taataatagt atagcattat
    28741 atagctattt cattgagttg gaagagtgct tatatccaga ttcactaaaa tttagaaata
    28801 aaaaatttga gaataagaac atataaaaat aaaaaattga attgaagcct agcttaatct
    28861 tttttgataa gatataagtt tgaactttat actaaatttt tagaattttc aatcaagaca
    28921 ataacataat agctaagcaa ttagaacaca attaagatgt ataaggcatg tatatgatga
    28981 atgggaggat ttaattaatg ttattagaga aattaagaaa agaagtatta caagcatctt
    29041 tagacctttt aaactataat ttagtgacat taacaggagg aaatgtaagt gggagagatg
    29101 aacaaactgg ctatatagca atcaccccaa gtggaatgga ttatagaaat cttacaccaa
    29161 gtgatattgt tatagtagat gtagatggaa atattataga tggaaaatgg aaagcatcag
    29221 tggatttatc agaccacttg tatatatata agcatagaga agacataaat agtataattc
    29281 atacacattc tacatactca agttgttttg ctattttaaa tgaacctata gaatgtgcaa
    29341 gtacaactct tgcaaatgaa gtagggggtt cagtaccagt tgcaaaattt tcaccaccaa
    29401 catctaaaaa gatgggaaaa tgtgttattg aagcaattgg tgacaaaaga gcatgtcttt
    29461 tagcaaatca tggcgtaatt gcagttggtc catctgtagg acatgctctt acagccgctg
    29521 taatgttaga agattcagca aaagtatatt acttagcaaa atcaattggc acaccagtgc
    29581 tactgcctga tgaagaaatt caaagagcga gagatgtatt ttttaatgtc tatggtcagg
    29641 ataaataaat tagataaaat tttaaaagta atatgaaaat atagatattc aatgatatta
    29701 aggagattta taatgatata ataagtttaa tagttaagaa gtaaaatatg aaatgtaata
    29761 gtatataaat tcatttaaga ctaaaaagaa taagataaaa taaaaaggag tctgtatttc
    29821 tatgtattac ttattaaaca aaagcacaaa tccttacttt aatttggcat tagaagaata
    29881 tctattttta aatgataaat ataatgatga tattataatc atatggagaa atgaagaatc
    29941 catttttata ggtaaaaatc aaaatccata tcaggaagta tatcatgatg ttattgaaaa
    30001 gggagaaatt cctattttaa gaagaatatc tggaggagga actgtttatc atgacttagg
    30061 taatatcaat atgtcattca tacaaaaaga taggcaactt catgagatag attttttgga
    30121 gcatactaag tttatgcaaa agatgctttc aaccttagga ctagatgtat ctataactga
    30181 aagaaaagat ttatttttaa atgggaaaaa aatttcaggc tctgcacagt ctataaaaag
    30241 aaaaaattct ctttatcatg gaactcttct ttacgattca gatttaaata aattaactaa
    30301 atatttaaat tcaaataaag caactgaatc aaatgcaact aaatctgttt caagtaaagt
    30361 tacaaatatt aagtttttac tcgaaaaaga tataaattac tttttagatt attgtgttga
    30421 atatttaaaa tctaatataa agaatataaa agaacttgaa ttggatgaag aagatattaa
    30481 aagtgtatac aatataggag aagataaata taaaaagata gagtggacat atggtaaaac
    30541 gccgaaattt caagtagtgc taccatttaa taatgaaagt aaaataaata tacatgttaa
    30601 cagatggaaa gtatcaaaat tttttatttc ttatgatgat aatgtaatta atatggacaa
    30661 gttgttagat ttgaatttct ttaaagaaga aataaaagag agtattttag aaaattatcc
    30721 acattacata gatttaatca atttaatatt ttaatataaa acctctaatt tgataaaatt
    30781 gtcttatatc aaactagagg tttttaatat tcttgcatat taacaaccat tacaaaattg
    30841 taaaattaaa aacaacacta ttgaaaaatc aatatatatt gtaataaata tatgtgtttt
    30901 tcattagcaa atagacatta actatttata caactgaaaa tttaatatat aattaatcag
    30961 atggttaaaa taaaaatatt aaaagtttta ataaatgatg gttaaaaata aaatacgagg
    31021 tgataccatg tatagtgtta taattgttgc agcgggaagc ggaaggagaa tgaacttaga
    31081 tataaacaaa cagttcataa aattaagaga aaaagaaatt attgctcata caattcaagt
    31141 tttttatgaa aatataaata tagatgaaat agtggtatgt ataaaaaaag aagaagaaga
    31201 tttttttaaa gaaaatataa taaataagta taactttaaa aatataaaaa tagcatatgg
    31261 cggaaaagaa agacaggact caatatataa tggtttaaaa aaattagatg aaaattgtga
    31321 tatagtactt atccatgatg gagcaagacc atttgtcgac catagaataa tcaatgaatc
    31381 tataaaggta gctaaagaaa aaaaggctgt ggttgtaggt gttccagtaa gtgatactat
    31441 aaagatagtg tcagatggaa cagtacaaga aactccagaa agaaatttgc tgtgggcagc
    31501 acaaacccct caaacctttg aatataatct cataatagat gcttatgaac aagcatataa
    31561 gaacaattat tatggtactg atgattcaat gttggtagaa aatataggtc aaagtgtcac
    31621 tatggtaatg ggttcttatg aaaacataaa aataactagt ccagaagatt taaatatagc
    31681 agaacaaata ttaaatatgg aaaaaagaga tgaagttaac agtagaagga gaattatatg
    31741 agaataggat taggatatga tgtacacaaa ttaacagagg atagaaaact tataatcggt
    31801 ggtgtagaga taccacacga taaaggtttg ttgggtcatt ctgatgctga tgtattgata
    31861 catgcaataa tggattctat tttaggtgct ctagcattag gagatatagg gaagcatttc
    31921 ccagatacag atgaagaata taagggagca gatagtatga aattattaga acatgtgtat
    31981 aatctaataa caagtaaagg atataaaatt ggtaatatag acagtactat aattgctcaa
    32041 agccctaaga tggctccata tattgagagt atgagaagta atatatcaaa agttttaaac
    32101 acagacattg ataatataaa cataaaggca acaacagaag aaggtcttgg ttttacagga
    32161 gcaaaacagg gaatagcttc acaaagtatt tgtttattat tgttgacaag tcagaataat
    32221 taatatatta ttagtaataa gtttaatgga tatcaagtaa taggcctata tagaatatct
    32281 ataatgtatg aatttaagtg aaattaatat aaatataaaa ttagctatga taaagaaata
    32341 gtaaaatgat gaaatcttca cagagagaaa acggtggtga gagttttcaa gaggaagtct
    32401 ttgaacctgt cttttagctt aaaggtataa aaatctttac ggtaggaacc gttatattcc
    32461 caaaagagag ctattcatat agctaactag agtggtaccg cggaaattaa acctttcgtc
    32521 tctatacttg tatagagatg agaggttttt tatattttca ggaagttaat attcaggagg
    32581 aatctattaa tgaaaatgtc aaaaatgttt atgccgacat taaaagaaat cccagcagat
    32641 gctgagatta caagccatca attaatggtt aggtcaggca tgataaaaaa aatgacttca
    32701 ggtgtttata accaattacc aatggggtta agagtgttca aaaaaattga acaaataatt
    32761 agagaagaat taaataaaaa agattgtcaa gaaatacttt gtgcagcctt acttccttct
    32821 gaattatgga aagagtctgg aaggtggact gctatgggtg aagaaatgtt tagactaaaa
    32881 gacagaacag aaagagagta ctgtcttggg ccaacacatg aagaagcatt cactgatata
    32941 ataagacaag aaattacttc atataaacaa ttgccactaa acttgtacca aatacaagta
    33001 aaatatagag acgagagaag accaagattt ggtgttatga gaactaagac ttttacaatg
    33061 aaggatgcat atagttttga tgtagatgat aaaggtttag ataagtcata tcaagatatg
    33121 tttgatgcat atgtaagtat atttgataga tgtggattgg agaatagccc tgtacaggca
    33181 gattcaggag ctataggagg ttctacatca gccgaattta tggttaagtc agaagttggt
    33241 gaagatgaag ttgtattttg tagtggatgt gattatgcag ccaatgtaga aagagcagaa
    33301 tcttgtaatc tagcatcaca aaaagaagaa atgaaagaat tagaagaggt tcatacacca
    33361 ggagcagcta caataaaaga gttagaagaa tttttaaaaa cttctccaga taagtttgct
    33421 aaaactttag tatatgaggc agatggaaaa acagttgtag tcgttgtaag aggagataga
    33481 gaagttaatg aaatcaaagt atctaatgcc ataggttctg ttatagaatt tgctcttgct
    33541 acagatgatg tagtgagaaa agtaactaat gcagaagtag gatttgcagg tccaataggt
    33601 ataaatgctg actatgtatt tatagataaa gaaattgttg aacaaagaaa catagttgtt
    33661 ggggctaata aaactgagta ccatataaaa aatgctaact atggaagaga ttttgaagga
    33721 atagttggag attttagaaa tgttcaagaa ggcgacaagt gtatagtatg tggtaagcca
    33781 cttgaaatag ctagaggtgt tgaagtagga catatattta aactaggaac taagtactca
    33841 gaatctatga atgcaaactt catagacaaa gatggaaaat caaagcctat agtaatggga
    33901 tgttatggaa taggtgttga aagaacagca gcagctataa ttgaacaaca taatgatgag
    33961 aaaggtataa tatggccttt gtcagtagct ccataccatg ttgtgatagt tccagcaaat
    34021 atgaaaaatg aagaacaaat atcaatagct gaaaatatat ataatgattt gcaagctatg
    34081 ggtgtagaag ttttattaga tgacagagat gaaagaatag gtgtcaaatt caatgattca
    34141 gaattaatag gaatacctat gaggattaca gtaggtaaaa atattaatga aggtaaagta
    34201 gaatttaaac ttagacataa agaagataaa gagataattg acatagaaga aataaatgaa
    34261 aaagtaaagg cagagtttat caggaataat gttaggttag gataataact taaataagat
    34321 attgtataga taatcaaagt tgtactttat agacaccatt ttatctatat ataaggatat
    34381 aaaatggtgt ttttttataa aacaaataaa tgaggaggta attaaaatgg caaaaaatga
    34441 aaaacaattt gttgaagaaa taacaaagat ggaagatgat tttccacaat ggtacacaga
    34501 tgtaataact aagactgact tagttgatta tgcaccagta aaaggtttta tggttgtaaa
    34561 gccatatgga tatgcattat gggaaaagat gcaagaattt atggataaaa aatttaaaga
    34621 aactggacat aaaaactgtt attttccatt attaatacca gagagtttat taaacaaaga
    34681 agcagaacac gttgaaggtt ttgctccaga agttgcatgg gtaacacatg gaggaaataa
    34741 aaaattagaa gaaagattat gtgtaagacc tacttcagag actataatat gtacaatgta
    34801 tgcgaaatgg ttaaaatcat atagagaatt accatatctt tataatcaat ggtgctcagt
    34861 tgttagatgg gaaaaatcaa ctagaccttt cttaagaaca tctgagtttt tatggcaaga
    34921 aggtcatacg ttacatgaaa cagcagaaga agcacaagaa gaaacaatac aacaattaga
    34981 agtatataaa gcgctatgtg aagaattact tgcaatgcca gttgtagctg gtcaaaaaag
    35041 tgaaagtgaa aaatttgcag gtggtgaaag aacatatact atagaagcta tgatgcatga
    35101 tggtaaagca ttacaatcag gtacaagtca cttcctaggt caacacttta ctaaggcttt
    35161 tgatattact tttgcagata gagaaggaaa tttagctaat ccatatcata catcttgggg
    35221 agcatctaca agacttatag gtggacttat aatgactcat agtgataaca gaggtttagt
    35281 attaccacca agagtagctc caattcaagt tgttatagtt cctatagctg ctaaaaaagg
    35341 aaatgtaatg gagactgttg ataaaatata tgctgattta aaagctaagg gagtagctgt
    35401 agaagttgat gatagagata actatacaac tggatggaaa tttaatgagt gggaaatgaa
    35461 aggtgttcca gtaagagttg aaattggacc aaaagatata gaaaataatc aagctatggt
    35521 atttagaaga gatactttag aaaaggattc tatgccttta gaaggattag ctgatgctat
    35581 atgtgattta tttgatgtta tacacaatga tatgtttgaa aaagcaagaa aacatagaga
    35641 agacaataca tctatagttg aaaatatgga tgagtttaga aaagctttag aagaaaagcc
    35701 aggattcata aagactatgt ggtgtggaga tgcagaatgt gaagctaaga ttaaggaaga
    35761 aacaggagca actataagat gcttaccttt tgaacaagaa aacttaggtc acaagtgtgt
    35821 gtactgtgga aaagaagcag atagtatggt agtaatggca aaagcttact aaaatacatt
    35881 taaataaaaa ctggggtaga aagtatttaa tactttatcc cagttttttt gttttttatg
    35941 agtaataaaa tgtatttgta actatgtcat aagataagtc atagatactt atattaaaaa
    36001 gaatataata gagaactatt tttaataatt acatattaat gaaagcaaaa tatttttgtt
    36061 atatagattt tcaataaagc aaacaataaa tataaatgaa tattagtaag gagtgctttt
    36121 aatttttaga aaataaatga cttaaaagaa agatttatga aattaaaact aggattttct
    36181 gatataataa acatgtgtat ttttaaataa aaaggatatt attattagat ttgattaaaa
    36241 ataaacttgc aaaacaatgt actagtttaa tattatagtt atattgaata aaatatcagg
    36301 aggaagtaaa aatgaatgta agagtaaggt ttgcaccaag tccaacagga tttgtacata
    36361 taggtagttt gagaactgct ttatataact atttatttgc aaaaaaaatg ggcggagaat
    36421 atatattaag agtggaagat acagaccaaa gtagattagt agaaggtgct atagagaata
    36481 tgcttaatgc tatgaaatgg gctggggtca accatagtga aggtgttata ttagatgata
    36541 gtggaaaagt agtacaaaaa ggagaatatg gtccatatat tcaatctcaa agattagata
    36601 tataccaaga atatataaag caacttttag atagtggaaa agcttattac tgtttctgta
    36661 caaaagaaag actggatgaa gtaagagatg ctcaaagagc agcaggagaa acagctaaat
    36721 atgatggtca ttgtaagaat ctttctaaag aagaagtaga agcgaatata aaagcaggaa
    36781 taccatatgt tataagatta agattgccag agaatcatac aattaagttt acagacttag
    36841 ttagaggaga tatggaattt aatactaatg acttagatga ccaagtatta atgaaaacag
    36901 atggatttcc aacttatcat tttgcagtag ttgttgatga ctatttgatg aaaattacac
    36961 atgttataag aggagaagaa tgggtatcat ctactccaaa acatgtttat ttatatgaag
    37021 cttttggatg ggaagcacct gtatttgtgc atttaccaaa tatacttaat aaagaaaaga
    37081 aaaaattaag taaaagacaa ggtgatgtag ctgttgaaga ctttaagaaa aaaggatatt
    37141 taccagaggg attagtaaac tatgtagcat tagttggttg gtctcctgaa gataatcaag
    37201 aattgtttac aatggaggaa ttagaaaaag ccttctctgt agaaagagtc tctaaaagtg
    37261 gtggagtatt tgatactgaa aaattaaatt gggttaatca gcattatata aaagatggtg
    37321 atgatgctta tctaacagac ttagcaatac catttttaat agaagatgga tttataacag
    37381 aagaagaagc tactaataaa tatgatttct taaagagtat gatatcagta cttaaagaga
    37441 aattacaata tgtaaaagaa gttactgaac atgcaagtat attttttgga gataaaatag
    37501 aggtagaaac tgaagaggga agagagttct taagattaga acatatacct actttaatag
    37561 atgctttgag agaaaaaata gaaaaaacag aggtattaaa tgctgagttt gttcaagcta
    37621 tgcttaaaga aatacaaaaa gagtacaaaa taaaagggaa aaatttattt atgggctcaa
    37681 gaataatttt gacagggcaa atgcatggtc cagatttacc aaaagtaatg gaagttttag
    37741 gaaaagaaac ttgcttaaat agaatagctt atgtaaaaaa taatatatta tagtcataaa
    37801 agtccgctaa tttagaagcg gacttttttt gtgttagtat ttggtgaaaa tatctgctta
    37861 agaaaatgaa ggtatttaag gtaataaatt taatatataa aaagcaaagc aaaaatataa
    37921 ataaaatatg aataataaac taataaaata ttaagcatag aagaagggaa tctattattt
    37981 tagtttgatt taattattaa gggggtaatt actatgtata gtaatattga tgatgttaaa
    38041 aaggaattaa aggagctatg cttagaatat gttacaattt tagaaaaatt aaaagatgaa
    38101 aaaatgatta cagaagaaac ttttgaaaag tgctcttctc aaaaaaaaat atttttagaa
    38161 gaacaataga ggatggtaat agtattatta ttatgtagtt tgaaagatag tacgaaaaat
    38221 gatagtaata ctattattta ttgcaatttt caactataat ataatatata atgttaagaa
    38281 gataatttat aaaaatacag tgaggtgatg agtgtgaaag tttataatgc attgactaga
    38341 acaaaagaag aatttgttcc attagaagaa ggaaaggtaa aaatgtatgt gtgtggtcca
    38401 acagtttata actatataca cataggtaat gcaagaccat ttataatatt tgatactcta
    38461 agaaggtatt tagagtatag aggatatgat gtaacttatg tacaaaactt tacagatgtt
    38521 gatgataaga ttataaatag aagtcatgag gagggaatat ctcctgaaga agttgcggct
    38581 aagtatataa aagaatattt tgtagactgt gatgggctag gaataaaaag agctactgtt
    38641 catcctcagg ttacagataa tatacaacag attatagagt ttataaaaga gttggaagat
    38701 aagggatatg catatgcagt aaatggtgat gtatattttg atacaaataa gtttgaaggt
    38761 tatggaaaac tttcaggtca aaaacaagaa gatttagagg caggggctag aattgaggtt
    38821 aatgaccaaa aaagacatcc tatggacttt gttctttgga aggcaaaaaa agaaggtgag
    38881 ccaggatggg atagcccttg gggagaaggt agaccaggat ggcatataga atgttctgtt
    38941 atgtctaaga gatatttagg tgaaacaatt gatatacatg caggtgggca agatttaaca
    39001 ttcccacatc atgaaaatga aatagctcaa agtgaagcta gaagtggaaa aacattctca
    39061 aaatactgga tgcataatgg atatataaat ataaatgatg aaaagatgag taaatcaaaa
    39121 ggtaatttct ttacagtaag agatatatca aaattatatg atttagagat agtaagattt
    39181 ttcatgttat cagcacatta tagaaatcca gtaaacttta gtgatgagat gttaaatcaa
    39241 gctaaagcag gtcttgaaag actatataat actaaagaaa aattagaatt tacgctaagt
    39301 aatttagtag aatctccttt aacagaaaaa gaagtagagc ttgttaaaga attagatgac
    39361 tttagacaaa aattcataga tgctatggat gatgatgtta atacagcaga cgcagtaagt
    39421 gtaatatttg aattagctaa gttaataaat tctaatgtag atgaaaattc ttctttggag
    39481 tttgctaaaa aatgcttaga tgaatttaat gaacttacag gagtattaaa tattgtaaat
    39541 aagaaaaaag atactgtatt agataaagac attgaagaat tgatacaaaa aagaactgat
    39601 gcaaagaaaa ataaagagtt tcaattagct gatgatatta gacaacaatt actagatatg
    39661 ggaattgtat tagaagacac aagacaaggt gttaagtgga aaaggatata aaaatggaaa
    39721 aaactgaatt agttactatg tcaccattag ttttagcata cttaggtgat acagtgtatg
    39781 agacatatat aagagaatat ttaattagac agaatacaca aagaaaagtt aatgatttgc
    39841 ataaactagc tataaagtat gtaaaagcta aagctcaggc aactataatt catgaaattg
    39901 aaattgagtt aactgaagaa gagagtaaaa tttataaaag gggaagaaat caaaagtcaa
    39961 atacttctcc taagaatgca gatattatag actacaaaca tgcaactggg tttgaggcat
    40021 tagtgggata tttgtattta aataatgaaa tagagagact tcaatatatt atcaataaag
    40081 gaatcaaaat tattgaaaga gatatgtaaa tattaaagag tgttgtctta ataataagta
    40141 ataaggcaac acttttttta taactaatgt ataataaata tatagaaatt agtaatgaga
    40201 aaggataaaa agttatgaaa gtaaaattaa tatcacatac acctgaacca gaaaaagtaa
    40261 tagctatggc agctaagcta tgttattcac cagttgggac agacgaaata gaaaaagact
    40321 taacagatga aagtatagag aaatttttaa atatgttatt aagtataggt catgggtcaa
    40381 tattagaaca tgcttcattt acattttcaa tagaaggtat ttcaagggct tgttcacatc
    40441 aaattgttag acatcgtata gctagttttt cacaacaaag tcaaagatat gttaaattag
    40501 aacaatttga gtacataatt cctccagaaa tagaaaaaat agaaaaggca aaagagttat
    40561 ttatagactc catgaaaaaa gaccaagaga attatgataa gttagttgaa atattgtttg
    40621 aaaatcatta taatgaccta ataaaaaacg gtaaaaatga gaaaacagct aaaagacaag
    40681 ctgaaaagaa agcaatagaa gatgctagat acgtatttcc aaatgcatgt gaaacaaaaa
    40741 tggtatttac tataaatgct agaagtttgt ttaatttctt tgaacataga tgctgtgaaa
    40801 gagcacagtg ggaaataaga aatttagctg tggagatgtt aagagaggtt aaaaaggttg
    40861 ctcctatatt atttaaaaag accggtccaa gttgtgtaaa tggaagttgt ccagaaggaa
    40921 ctatgacttg tggagatata gttcaagtta gagaaaaatt taaggccttg taggaggtga
    40981 ataatttggc gagtatagaa ggaagaaatc cggtaataga agcaataaaa agcgacagag
    41041 aaatagataa aatattaatt gcaaattcag ctaaagaagg ttcaattaag aaaataatag
    41101 gaatggctaa agataagaat ataattattc agtatgttga taaacataag ctagatgaag
    41161 taagtacaag tcattcacat caaggtgtaa tagcttatgc aagtgagtat aagtattatg
    41221 aattagatga gctaatagat ttagcaaaga ataaagatga agacccattt ttcattatat
    41281 tagatgagat aacagaccca cataatttgg gttcaataat aaggacagca gatgcagtag
    41341 gagcacatgg agttatcatt ccaaaaagaa ggtctgtaca cataactcct gtagttgcca
    41401 aagcatctgc tggagcagta gaatatatgc cagtttgcaa ggtaaccaat atagttaata
    41461 caataaaaag actcaaagaa gaagggctgt ggattgctgc agctgatatg gatggagaaa
    41521 ctttttataa acaaaatctt actggtcctc ttggtgtagt aattggaagt gaaggttttg
    41581 gaatatctag attggttaaa caaaactgtg actttatagt aaaaatgcca atgataggta
    41641 atgttacatc tttaaatgca tctgtagcag gtggaatact tttatatgag atattcagac
    41701 aaagactgga taaaagtaaa taggcatgaa aaaaaatata aatcattact taataataga
    41761 tggctacaat attataaatg cgtgggacaa tttgaaagaa ctggctaaag aggacttaga
    41821 agattctcga gaaaagctta ttgatgacat tatagaattt tctgagttta tgggttataa
    41881 gactataata gtttttgatg cttataatgt taaaaattct cgagaaaaag ttgaaaaaag
    41941 aaaacacata acaatagttt atacaagaga acaccaaaca gcagatagtt atatagagaa
    42001 atttataact tctttatcaa agtatgatga tgtaaaagta gccactaatg actatgcaga
    42061 acagcaaatg attttaggaa aaggtgctac tagaatgtct gcaagagaat taaagttaga
    42121 gttagatcgt tccaagaata aaatgaagga aaaaaatatc ggtttgagga aaaaaattca
    42181 gcgaaattgg ttggaggaaa ggttagataa agaaacattg tcgaaacttg agaacattcg
    42241 tagaaagcgt tgaaattact aggttcttta gactataata tacctataaa tttgttttat
    42301 atatggggga gatttacatg ttggtagcaa aagaaaaaag ttatgagtta gtggacaatt
    42361 gtcagcagga tgagtataac attgtattaa gagcaagtga aggggataaa atagcactag
    42421 agtacattat tactaaatac agaaattttg taaaggcaaa agcaaagtca tattttttaa
    42481 ttggagcaga taaagaagat ataatacaag aaggaatgat aggcctttat aaggcagtta
    42541 gagattttga tggaagtaag accaattcat ttaaatgttt tgcggagata tgtataacaa
    42601 gacaaataat aactgctata aaaactgcaa caagacaaaa acatatacct ttaaattcat
    42661 atgtttcttt gaataaacca atatatgatg aagagtcaga cagaacactt ttagatatta
    42721 tagctacaag tatagtaaca gatccagaag agcttataat tagtaaagaa gagttaaaaa
    42781 atatagaatc aaaaatgaac gaattgttaa gtgacttgga attagaagta ctagaattat
    42841 atttaaatgg aaagtcatac cagtttatag cggacaagct taaaagagat gtaaagtcaa
    42901 tagataacgc tttacaaagg gttaagagaa aattagaaaa acatcttgaa aacagaaatg
    42961 attaatagta aaatacttac cataagctta ttgtgagcaa aattaattta tatcgaaaga
    43021 taagggagga aattcaaaat ggctaaagct aaatacgaaa gaacaaaacc tcatgttaat
    43081 atagggacaa taggacacgt agaccacggt aaaactacat taacagcagc aataacaaaa
    43141 acattatatg acagatatca attaggagaa gcagtagatt tcgcaaacat agataaagct
    43201 ccagaagaaa gagaaagagg aatcacaata tcaacagcac acgttgagta tgaaacacca
    43261 a
//
LOCUS       DAMKMI010000017        40762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMKMI010000017 DAMKMI010000000
VERSION     DAMKMI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.017"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            152..811
                     /gene="cprR"
                     /locus_tag="PC429_000884"
     CDS             152..811
                     /gene="cprR"
                     /locus_tag="PC429_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF3846402.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1022..2080
                     /locus_tag="PC429_000885"
     CDS             1022..2080
                     /locus_tag="PC429_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF3846403.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2331..4475
                     /locus_tag="PC429_000886"
     CDS             2331..4475
                     /locus_tag="PC429_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2745..2747,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDF3846404.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4524..5357
                     /gene="fdhD"
                     /locus_tag="PC429_000887"
     CDS             4524..5357
                     /gene="fdhD"
                     /locus_tag="PC429_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF3846405.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5368..5541
                     /locus_tag="PC429_000888"
     CDS             5368..5541
                     /locus_tag="PC429_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846406.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5599..6150
                     /locus_tag="PC429_000889"
     CDS             5599..6150
                     /locus_tag="PC429_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3846407.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRQCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6175..7551
                     /locus_tag="PC429_000890"
     CDS             6175..7551
                     /locus_tag="PC429_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF3846408.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7551..8066
                     /locus_tag="PC429_000891"
     CDS             7551..8066
                     /locus_tag="PC429_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3846409.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNLDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8144..9427)
                     /locus_tag="PC429_000892"
     CDS             complement(8144..9427)
                     /locus_tag="PC429_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF3846410.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9615..10037
                     /locus_tag="PC429_000893"
     CDS             9615..10037
                     /locus_tag="PC429_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF3846411.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10139..11077
                     /locus_tag="PC429_000894"
     CDS             10139..11077
                     /locus_tag="PC429_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF3846412.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11263..13287
                     /gene="ligA"
                     /locus_tag="PC429_000895"
     CDS             11263..13287
                     /gene="ligA"
                     /locus_tag="PC429_000895"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF3846413.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13447..14796
                     /gene="rph"
                     /locus_tag="PC429_000896"
     CDS             13447..14796
                     /gene="rph"
                     /locus_tag="PC429_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF3846414.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKIIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14796..15266
                     /locus_tag="PC429_000897"
     CDS             14796..15266
                     /locus_tag="PC429_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3846415.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15460..16746
                     /locus_tag="PC429_000898"
     CDS             15460..16746
                     /locus_tag="PC429_000898"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF3846416.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16896..17480
                     /gene="clpP"
                     /locus_tag="PC429_000899"
     CDS             16896..17480
                     /gene="clpP"
                     /locus_tag="PC429_000899"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF3846417.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17499..18749
                     /gene="clpX"
                     /locus_tag="PC429_000900"
     CDS             17499..18749
                     /gene="clpX"
                     /locus_tag="PC429_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF3846418.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18820..19347)
                     /locus_tag="PC429_000901"
     CDS             complement(18820..19347)
                     /locus_tag="PC429_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF3846419.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19335..19847)
                     /locus_tag="PC429_000902"
     CDS             complement(19335..19847)
                     /locus_tag="PC429_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF3846420.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            20027..22390
                     /gene="lon"
                     /locus_tag="PC429_000903"
     CDS             20027..22390
                     /gene="lon"
                     /locus_tag="PC429_000903"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF3846421.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22380..22991
                     /locus_tag="PC429_000904"
     CDS             22380..22991
                     /locus_tag="PC429_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF3846422.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23257..24627)
                     /locus_tag="PC429_000905"
     CDS             complement(23257..24627)
                     /locus_tag="PC429_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3846423.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFVGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24802..24917)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25205..26284
                     /locus_tag="PC429_000906"
     CDS             25205..26284
                     /locus_tag="PC429_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3846424.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26676..27356
                     /locus_tag="PC429_000907"
     CDS             26676..27356
                     /locus_tag="PC429_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF3846425.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27349..28728
                     /locus_tag="PC429_000908"
     CDS             27349..28728
                     /locus_tag="PC429_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF3846426.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28899..29984
                     /locus_tag="PC429_000909"
     CDS             28899..29984
                     /locus_tag="PC429_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF3846427.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            30038..30397
                     /locus_tag="PC429_000910"
     CDS             30038..30397
                     /locus_tag="PC429_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF3846428.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30450..31322
                     /locus_tag="PC429_000911"
     CDS             30450..31322
                     /locus_tag="PC429_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF3846429.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYISNIGMII"
     gene            31323..31766
                     /locus_tag="PC429_000912"
     CDS             31323..31766
                     /locus_tag="PC429_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846430.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINIITKISIDK
                     NKLEIEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31781..32365
                     /locus_tag="PC429_000913"
     CDS             31781..32365
                     /locus_tag="PC429_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846431.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANLLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32618..33655
                     /locus_tag="PC429_000914"
     CDS             32618..33655
                     /locus_tag="PC429_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF3846432.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33736..34101
                     /locus_tag="PC429_000915"
     CDS             33736..34101
                     /locus_tag="PC429_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846433.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34106..34423
                     /locus_tag="PC429_000916"
     CDS             34106..34423
                     /locus_tag="PC429_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846434.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34471..36075
                     /locus_tag="PC429_000917"
     CDS             34471..36075
                     /locus_tag="PC429_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846435.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36199..37827
                     /locus_tag="PC429_000918"
     CDS             36199..37827
                     /locus_tag="PC429_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF3846436.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAALDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNIYFSEEGQSELD
                     VMYNRVVSAYTYALESMRSDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38092..39441
                     /locus_tag="PC429_000919"
     CDS             38092..39441
                     /locus_tag="PC429_000919"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF3846437.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39597..40676
                     /locus_tag="PC429_000920"
     CDS             39597..40676
                     /locus_tag="PC429_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF3846438.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16750 a         4306 c         7345 g        12361 t
ORIGIN      
        1 aaagtttttg tatctaaaat attttatttg taaaatagta attaattatt gtaagctata
       61 atatgagagt attatggtta atgtgaattt taaagatatt attaaatata taaattagaa
      121 tactattaaa cggataaatg gaggagttat tatgtctaag atattagtag tagatgatga
      181 gttagatatg ttaaagttga ttgaaaatgt gttgaaaaga gatggacatg aagtaaaggt
      241 aattagtgat gtagaaaaag ctatgaacat ggattttaat tattttaatc taataatatt
      301 agatgttatg atgcctaaga tggatggttt tgaagtatgt aaaactataa gaaataaggt
      361 tgattgtcca atactgtttt tgacagcaaa aaatatggaa agtgatatta tgtacggtct
      421 tggtataggt gcagatgact atataaccaa accttttggt gtaggagaat taagggctag
      481 gataactgct catttaaggc gagaaagtag agaaaaaaag aatttattaa ctatctcaaa
      541 tgttcaattt aactttttag gcaaagaagt ttcaattgat ggtaaaaaaa ttaattttac
      601 taaaagtgaa tatctaatat gtgaatttct agcaaaaaat aagggacaag tattttcaaa
      661 agaaagaata tatgagtcgg tatatggttt tgatggagaa agtgatataa gtactatcac
      721 agagcatata aaaaatatac gaagcaaatt aaaacaatat gatatttata caatagaaac
      781 tgtatggggg atagggtata aatgggtgta ataagtataa gtgttgttta gtgaaaatat
      841 tatatatatc tacaaaatgt ttgtaaaata ttaatctata tattaaaatt taatttatag
      901 attaatattt tttgtaaatg acaaaattgt aaggtatttt gttataaaat attaagattc
      961 atagtgtata ctaataatat agtatttagt ataaatcttg agcaaaggat ggttatttct
     1021 aatggataga ctaaagaata ttagaaagag tttactacag tatacatttt tatttttgtt
     1081 aataggtata acaatttacc tagtgtttaa aacattggat attaaaatgc tatcaaatgt
     1141 tatggttatg gtagataaga agtttttatt cataggtgct ttggcaataa tgtgtcacat
     1201 aatgcttgaa ggagtagtaa tgaagataat tatagaaagc actcacaaag ttaatattag
     1261 atttattggt tttaagctag ccattatggg attttattat aatttaataa ctccttttgc
     1321 ttcaggaagc caaccagtac agatatatgt acttaaaaaa tgtaaaatgc cacttagtaa
     1381 ggcaagtgca gttgttacca ataagtctat tatcttccaa atagtagtta ctttttattg
     1441 tacaatactt gttttaatga attttgctat gttagaaaaa caaatgaaaa tgattatgcc
     1501 tctagtgttt ctagggatag ccattaattc atttgtctta attatgataa tattagttat
     1561 tttaaatcct aaaaaaataa aatattttat gagaattact ataaagcatt tatcaaaaat
     1621 taagttttta aaatttttag ataataagat tgaatccata gaaagtttta tagatgatta
     1681 tagtaaagca ataaattttt ttgcaaagaa taaaaaggta ttaatttcaa ctataatagt
     1741 aacttttatt caattaagtg cttactttag tgtttcattt tggatatata aagcatttaa
     1801 tttacaaggt tatagctata tttatatact gacattacaa gcatttttat atatggcaat
     1861 atctccaata ccaacacctg ggaatgtagg agctaatgaa ttagcctttt tcactatatt
     1921 caaatcagta ttccctcaac ctttgatggg atatgcagta tttttatatg gtggatttat
     1981 gtactatttg attctgattg gaagtggaat atttactgta attactcact atagaatgaa
     2041 aaatagaatt ggcagaaata ttgttttaag tgaaaactaa ataagtttaa agaatatata
     2101 ttgagtatat taaaaaatat gtcataagaa ctagaatatt ttaattaaat ggttgaaata
     2161 taaagattat tatgacatat tttttttgaa atttaagagg aattgtgaga aaattgttga
     2221 atttaataga tagtattgca aaatatttat gattttgcat attataaaaa cactcattat
     2281 ctttaagata aattttactt atcaggggat aaatctaagg ggggaattgt atggagaaaa
     2341 aaattttaac agtttgtcca tactgtggag ctggatgcca attatatcta gtagtaaaag
     2401 ataataagat attaagagct gaaccggcta atggtagaac aaatcaagaa agtttgtgtt
     2461 taaaaggtcg ttatggatgg gattttctaa atgaccctaa aattctaaca cctaggctaa
     2521 aaaagccaat gataagaaaa caaggtgtac tagaagaggt tgaatgggat gaggctataa
     2581 actatacagc ttcaagacta aatgaaatta aagaaaaata tggtcctgat tctattatgg
     2641 gaacaggttc tgcaagagga cctggtaatg aagcaaacta tataatgcaa aaatttatga
     2701 gagctacaat aggaactaat aatgttgacc attgtgctcg tgtctgacat gcaccatctg
     2761 tagccggtct ggcttactca ttaggtagtg gtgcaatgtc taattctata ccagagatag
     2821 aaaatgcaga tgttttattt atatttggat ataatggagc agattcacat ccaatagtgg
     2881 caaatagaat agtaaaagct aaaaaaaatg gtgctaaatt aattgttact gaccctagag
     2941 taactgaatc tgcaagaatt gctgatatac atctacctat aaaaggtgga acaaacatgg
     3001 ttttagtaaa tgcttttgga aatgtattaa tagaagaagg tctttacaat aaagaatttg
     3061 ttcaaaatca tacacaagga tttgatgagt ataaagaaat tgttaaacca tatacagcta
     3121 aatatgctga aaagattaca ggaatcccag aagagcttat aagaaaagct atgagagaat
     3181 atgcaaaagg taaaaaagct atgatacttt atggaatggg tgtctgtcaa tttggtcaag
     3241 ctgttgatgt agttaaggga cttgcatcaa ttgctctact tacaggtaat tttggtagag
     3301 aaagcgttgg aataggacct gtacgtggac aaaataatgt tcaaggagct tgtgatatgg
     3361 gagctcttcc taatgtatat cctggctatc aaaatgtaac tgatgataag ataagagaaa
     3421 agtttgaaaa agcttggggg gtaaaattat ctcctaataa tggttatagt ttaactcaag
     3481 ttccaaattt agtactaaaa gagaaaaagc ttaaagctta ttatatattt ggagaagacc
     3541 cagtgcaaag tgacccagat gcatcagaag ttagagaagc attagatgaa ttggaatttg
     3601 tcattgttca agatatattt atgaataaga cagcacttca cgctgatgtt atacttccag
     3661 ctacttcttg gggagaacat gagggagtat atacatgtgc agatagagga ttccaattga
     3721 tgagaaaagc tatagagcca caaggagatg taaaaccaga ctggcaaatt ataagcgaaa
     3781 tttctacagc aatgggatat cctatgaatt ataaaaatac taaggaaata tgggacgaac
     3841 ttagacaatt atgtccaagt ttcttaggtg ctacttatga aaagatagaa actcaaggat
     3901 gcgttcaatg gccttgtaag agtgaaagta tggaagataa aggaactatg tatctgtatg
     3961 aaggtcaaaa gttttctact cctaatggta aaggtaattt gttcgctgct gaatggagac
     4021 cccctatgga agttgaagat gatgaatacc catttagttt atgtactgta agagaagttg
     4081 gacactattc agtaagaaca atgactggta attgtagaac acttagttca ttagaagatg
     4141 aaccaggacg tgttcaaata aattctaatg atgctgaaaa attaggaatt gaagatgatg
     4201 aattagttag aattagttca agaagaggaa gtgtaataac aagagcaaca gttactgata
     4261 gagttaagga aggtgcaaca tatatgactt atcagtggtg ggttggagct tgcaatgaac
     4321 ttacaatagc aaatttagat cctatatcta aaacaccaga gtataaatat tgtgctgtta
     4381 aacttgaaaa acttgaagac caagaattag cagaaaaatg tgtaagagaa gagtatcaaa
     4441 gtttgaaaga caaaatgaca gctacaaata tctaatgatt tattagattt aagatttata
     4501 aaaggcagaa aggctaatat tttatgaaca atatatactc aaaaactaaa ttacaagaaa
     4561 atagtaagga taaagttttt gaaaatagtt atagtctagt ggactacaaa tatttaaatt
     4621 cttttaatgt agaagtaagc aaaataaatg aacatgaagt atcaatagat aatgaagaaa
     4681 taattgcaga atatccactt agttttattt taaatgataa atatataaat acatttttat
     4741 gtaccccata taatttagag gaacttatag taggattctt gaaaacaaaa ggatacatag
     4801 aaaacaaaaa aagcttatta aaatttgaga taaatgagag tgaacgtttt gcaaaagtaa
     4861 ctattcaaga gaaacaaaaa aatcaagatg agcaaattat atttttaaat tcactagact
     4921 atataacatg tgctcctgta aagagcaata taaaaataaa ttatgatacg atttacagta
     4981 taatggatag aaatttaaat tcatcagaat tatttaaaaa tactggtggg gtacatagcg
     5041 tatctgtatt taattttgat aaaaaaatag tcacttgtga agatgtagca aggcataatg
     5101 caatggataa ggctattgga ttttgtgttt taaatgacat aacattaaaa gataaaattg
     5161 tggctgtaag tggaagaata tcattggaaa tgatattaaa agcagctatg actcaaattc
     5221 ctattgtagt atcaaaatca gcaccgacta atttatcaat agagctttct aaaaaattaa
     5281 atataacgct tattggattt gtaagaggaa gacggatgaa tatttatgca aatccacaga
     5341 gagttatcaa aaagtgagaa tgaatatatg tgtactaaaa aagaagagca aagatattca
     5401 aagtgtgaaa tctgtggagt attaattgat gatactgata aggtgtataa taacacatac
     5461 aatttgtgca aaaaatgtaa acgaaatata agtactgaga aaatagtgaa atatataaat
     5521 atatatgatg aaagtaagta gacatatgat gaaaataact agagggttgg aaatatacaa
     5581 attgaaaggt gggataatat gaaaaataaa ataggctctt ttgtaatagc agacccaaac
     5641 aaatgtacag gttgtagagc ttgtgaagta gcttgtttta caatgcataa tcagcataat
     5701 aatgtgggtt atacagttgg aacagttgat atacctgtta tacctagact ttacttagta
     5761 aaaggtgaca atttctgtat gccaatacag tgtagacagt gtgaagatgc tccttgttta
     5821 aactcatgtc cacaaaaagc tatagttaaa gaaaacaata ttatgtctgt aaatgaagaa
     5881 aagtgtatag gatgtaaaac ttgtctgcta gcatgtcctt ttggagctat agacttactt
     5941 ccacaatatg aagatggtaa agaagtagaa caagtaattt tagatgaaaa taagaagata
     6001 gcttataaat gtgacttatg taaagacaat gaaaaaatag cttgtataag tgcatgtcct
     6061 caggaagcat taaaacttgt tactccaata gatgacaaaa aagctaagaa tagacaagct
     6121 gcacttagtt tattgttaac aaataaataa aggatacagg ggtaggtgac gtttatgata
     6181 ataaatatag ataaggattt atgtactgga tgtagagaat gttcaaaggt ctgtccagta
     6241 aatgctattg aaggtgaaga gggaaagcca caagagataa atttagatag atgtgttatg
     6301 tgcggtcaat gtgttcaaac ttgtaaatct tatgcttcag ttatagatga aggatttgag
     6361 tttttacaag agaaaaaaca agaaagagag ataccagagt ctataaatga acctatattt
     6421 gcagcataca atgtatgtga tatagataag gtgaagaaag ctttaagtga cccaaatgta
     6481 ttcactatgg tccaatgtgc accagcagtt agagttgctt taggagaaga tttcggatat
     6541 tatttaggat acttaggtgc tgggaaaatg gcagcagcac ttagagcttt aaattttgat
     6601 agagtctacg atactaattt tgcagcagat ttaactataa tggaagaagg tagtgaatta
     6661 attaaaagag tgacagaagg tggaactctt ccaatgttta catcttgttg tccagcttgg
     6721 gtaaaattca tggaaaaaaa ttatcctaaa ttaactaatc atttatcttc ttgtaaatca
     6781 cctcagcaga tgggtggtgc aatatttaaa acttatggtg cagagataaa taatgtagat
     6841 gcatcaaaga tatttagtgt ttctataatg ccatgtacat gtaaaaaata tgaatgtgat
     6901 agagaagaaa tggattctag tggatataga gatgtagatg tagtattaac tactagagaa
     6961 ctagcttatt taataaaaga catgggaatt gattttaaaa atttaagaga agaaaaattt
     7021 gatagtcctt taggtagtta tagtggagct ggaactatct ttggtgttac tggtggggtt
     7081 atggaagctg ctataagaac aggatatgag cttataactg gagaaagtat tcctgatgta
     7141 gaagtaaaac aagtaagagg agaaaatggt tttagaaagt caactattaa aataggagac
     7201 ttagacttaa gagttggagt agtaagtgga ttaaaaaatg taataccagt gcttgaagac
     7261 ttggaaaaag gaaaattaga tgtagatttt attgaagtta tgacttgtcc tgttgggtgt
     7321 gtaagtggtg gaggtcaacc aaaaatttta ctagaagaat ataaggaatt ggcttatgaa
     7381 aatagaacaa aagcaacata cgtgcatgat gaacatttag caattagaaa atctcatgag
     7441 aatcctgata ttaaaaaact gtatgatgaa tatctggtag aaccattagg agaaaaatct
     7501 catcatctat tacacactac atattgtata ggaaaggaag tggctaagtg atgaactatt
     7561 ttgttatagc agaccctgat aaatgcatag gatgtagaac ttgtatgata ggatgtgtag
     7621 ttgcacattc agatgaagat attttttacc aaaatctaga tgaaataaat tttaatccaa
     7681 aactttctgt cataaagaca aaagaagtaa gtgctccaat acagtgtaga cattgtgaag
     7741 atgctccttg tgcaaaggca tgtccaaatg gtggtatagt tagggttggg aacacaataa
     7801 aaataaatga agaaaattgt ataggatgta agacttgtat gttagcatgt cctataggtg
     7861 caatagacat agttacatta aaagatgtag atgaaggaaa gctttgcttt agagaaagaa
     7921 tgacagcaaa taagtgtgat ttttgtatag aatcgccaga agggcctgca tgtgtaaatg
     7981 tttgtccaac taaggcattt actatagtta aggatgagga tatagatagt aaagttaaaa
     8041 ataagagaaa attagctata ttgtaatata aaaaagatgg agttttattt atgattaaaa
     8101 gctccatctt ttttattata atatttatat atctaaaaat caattattgc attagtttat
     8161 ctaatctttc tttattcaat tttttcattc ctatagaagt tataataagt aaagtatagg
     8221 caattataac tgataaaatt ataatagacc aagaaaatct gtatccaaaa ttatcaacta
     8281 acaatccaaa taatgttgaa cctaatgcaa atcccactgc aaaaattaat tgtacgattc
     8341 catatatgct accaaactcc ttttgaccaa agaaagagcc agttaaataa gcaggaccaa
     8401 tcatataagc aaatacagaa agacctttca aaatggcaaa tacaaatgca aaagtagaag
     8461 atttatctac caatagtaat gataaagttg ctataagaac caatacaaaa gatataataa
     8521 gtggctttgt ttgaccgaat ttatcaaaca ataatcctcc aaataaatta ccaatgagag
     8581 caaataaagc aaatgtggag cctactgttc ctattagaat aggatttaga taatttctaa
     8641 gataagttgg atattgtacg gataatgatg aaacatatat accaacaaat ataaatccaa
     8701 tagtcataat ccaaaaatat ttaatttttt tagcttcttt taatgtataa ccccatactg
     8761 aaggagaaat tgtatcagat gctttctctt ttttaccttt agattttacc ttttcattaa
     8821 tatctttagg catcctgagt atgaataata ttacaggtat accaactatt aatgctataa
     8881 ttccaaatat tagataagaa cgactgtatc cttgagttgc taataagcga actgcaagtg
     8941 gttgtagaaa tatactacca atagaaccac cagcaaaagc aaagcccaaa gcttttcctt
     9001 tgctttcttc atcaaaccaa gcacttatta aaacaggtat tcctatagca gatataattg
     9061 tagcgccaat ttgaacaata catgatacag tgtaaaattg ccatagttca gtacatatag
     9121 aaaaagtagc aaaagcaata ctagaaatta tacaacctaa taaatatagt atcttaatat
     9181 tgactttaga aaataataca cctataaaag gtgatgcaat tgctgaggca atagtaccaa
     9241 tagtgaatat tagagaaaaa gaagctaaac taaagccttc tccttctaca acataactta
     9301 taaattgagg ctgtatactt gctacaatac taaatggaac agcttgtatc aacatacaac
     9361 caagtactat aacccaagca tttgaaaagt ttttaatgct aaatttattg tttagtaatg
     9421 tattcatggt atctctcctt taggttatga tttgtagata ttaatctaat tattctaatt
     9481 ttatatatta gttttggata gcccaactat ttaaatataa tttgggtatt aatataaaaa
     9541 ttaactataa aaattgatta ttttaaatgt atggggtaaa ttaaagtata taaataaatg
     9601 aaaggatgga taaaatgtca tttgaaaaaa taaataataa taacctagat aaaatacaca
     9661 acaggaagtg tattatgctt gtaaacttta ataaaaaaga attatcatta attaaaaacg
     9721 taagtaactt tgctggaata agagaccata tcatcttaag tgataaaaat ggagagaata
     9781 taataagaga cattttagat ggaaatatat caaatgaatg tgaaaatggg attaagagta
     9841 agtctgtaat atttaataac actccaagca cacaagtaaa tgcattttta gaagggctaa
     9901 aaaaagtgag aataaataga ccttttacag ctatggtaac agagacatct attgattgga
     9961 cattgaataa tttaatagaa aatttagttc aagaacgtaa tgctatgtct ttaggaaaaa
    10021 caattgaaca taagtaaatt taagatatct tatagtaatt ttaactaatg tacattgagt
    10081 atcatattat attttagaat ataaaatttt aaatgtgggt agatggagga gatttgagat
    10141 gaaagtttta tttacaataa attatggaaa agaaaaattt gataaaataa gagaattagg
    10201 ctatgatgtc atacattact ctgaagatag tattgaaaat aatgaggatg taaatacagc
    10261 agatgtacta gttacataca atccatttga gagattagat atttctaaga tgaaaaattt
    10321 acaatatata cagacaacta gtgtaggtat agaccaactt ccaaaagaag aggtattgaa
    10381 gagaaatatt aagatagcta ataatagagg cggatatagt gtacctattg gtgaaagtat
    10441 tgtattgtat attttagaaa tatataaaaa tagtgcaaaa ttttttaaac aacaacaaaa
    10501 taaagaatgg aagatggatt tttctgtatc agaattgtat ggacaaagag ttggattttt
    10561 aggaacaggt actatatctt cagaagcagc aaaaagatta aaagcttttg gtgtagaaat
    10621 atggggtgtc aatactgatg gaagtagtaa aaaatacttt gataagtgtt ttgctactga
    10681 agatatgaat attgtatttg aagaatgtga tatagtagtt gtagcaatgc cttctacaaa
    10741 aagtacagat ggaatagtaa acaaagatat gtttaaactt atgaaagatg gttctgtatt
    10801 tataaatgta ggtagaggaa atacagttaa tcagaaagat ttagaagaat gtgtagagaa
    10861 atttagaggg gtagcattag acgtatttga aagtgaacct ttaaacaagg acaataagtt
    10921 gtgggagtgc gaaaatgtca tagtaactcc tcataattct tgggtgtcag ataaaaacaa
    10981 agagagaaca tttaatatgg tttataataa tttgaaacac tatataatgg agaataaacc
    11041 tgtagataat gttgtagaca tatcaagagg ctattaaaaa taaaaaattt tgataaaaat
    11101 aaaaactctt attagtcgta ttaaagatac taataagagt tttttttgta ataaatttaa
    11161 agcccaaaaa gtgatatact aaaaaatata taaataggag ataattaatt atagggaaat
    11221 ttcaaaataa tttaatttta aaaatatgaa gaggtgcaaa aagtgagcgt aaaagatgaa
    11281 attaataaac ttatagagac aataaactac cataatgaaa ggtattataa tcaagatagc
    11341 ccagaaatat cagattatga atacgataag ttaatgaagg agttaatttc tttagaagaa
    11401 gaacatccag aactaaagag aattgattct ccaacaaata gggtaggagg taagccatta
    11461 gataagttca atcaaattgt acataaaact cctatgttga gtttatcaaa tgctttttca
    11521 gagcaagatt tgagagattt tgataagaga gttcaagaat atgtgggaga aaatgttgaa
    11581 tatgttgttg aatttaaaat agatggtctt tctgtaggtc taacttataa caatggagag
    11641 tttgaaaaag gagcaactag aggagatgga gttgtaggag aagatatttc tcaaaactta
    11701 atgacagtaa aaagcatacc tcttaagata aatgataaaa aagaactaat agttagagga
    11761 gaagtatata tatcaaaaga taattttaga aaagtaaatg aacaacaaga agaacaagaa
    11821 cagccattat ttgctaatcc aaggaatctt gcagctggtt cattgagaca attagaccca
    11881 aaactaacag caaagagacc attagatata tttgtattta atatggaaaa tatagaagaa
    11941 tacgatttag aaagtcacag tgaatcacta gagtacttag aaaaactagg attttcagta
    12001 agtcagagtt ataaagtttg taaaagtata gatgaagtta ttgagcatat tgaatactgg
    12061 acagataaca ggggtaattt agactacgaa atagatggta tggtaataaa agtaaacaat
    12121 ataaaacaaa gagacgaaat gggatataca gccaaaagtc ctagatgggc aattgcatat
    12181 aaattcccag cagaaaagaa gaaaactaaa atagtagata taatagtaga agttgggaga
    12241 actggtacaa taacaccttc agcaattctt gaaccagtca gacttgcagg tactactgta
    12301 agtagagcta cacttcacaa tgaagattat ataagagaaa aagatataaa aataaatgat
    12361 acagtattag ttcaaaaagc aggggacata atacctcaag ttttagaagt tataaaagaa
    12421 gaacgtactg gagatgaaat agattttaaa atgccagaaa agtgtccagt ttgttcagag
    12481 ccaacaataa gattagaagg agaagctgct gtaaaatgta tcaatatgtc ttgcccagcc
    12541 caaataagaa ggggaattat acactttgtg tcaagggatg caatgaatat agatggattg
    12601 ggagaatcta taattacact gctattaaat gaaaaaatta ttcaagatgt atctgattta
    12661 tattatataa aaaaggaaga tgtagtcgac ctagagagaa tgggcgaaaa atctgctgac
    12721 aatttaataa attcaattga aaaatcaaaa gaaaatgatt tatggagact tataaatgga
    12781 ttaggtatta aatttatagg agttaaagca gctaagatac ttgcatataa ctttaaggat
    12841 ttagatgaag taatttctgc aactagtgag caactagaag aattagagga atttggaagt
    12901 ataatggcaa acagcgttgt cgagtttttt aaggaagaca aaaatatgaa cgttataaac
    12961 aagttaaaaa atgtaggagt aaatacaaaa tcattggata atacaggtga aaaagtagaa
    13021 aaaatatttg aagggatgaa aatcgtattg actggtacac ttcctacatt aaaaagaaat
    13081 gatgcaaaag atatgataga aaaaagaggt ggaaaagcta catctagtgt tagtaaatcg
    13141 acaacatttg ttttggcagg tgaagaagct ggttctaaac ttactaaagc aaatgattta
    13201 ggaattaaag ttatagatga agagagattt ttagaattat taaagttatc atcgaaagat
    13261 gaagtaaaag ctgtattaga gagttaaaaa gtcgcttaat ttagtagaaa actactataa
    13321 ttaatgatag aatagttcag taagctaata agattacgaa aaatgacaat aattaatatt
    13381 tgacaaaatt aaaaattata aaaataaatt tataaaaaat aaaaaatatg agaaaggata
    13441 taaaaaatga ccagaagtta tgatagaagt aacgaacaga taagacctgt aaagataact
    13501 agagattata caaagtatgc agaaggttct gttcttatag aaatgggaga gacaaaggtt
    13561 atatgcactg catcagtgga agaaaaagtt cctccttttt taagaaatag tggtactgga
    13621 tggataaatg ctgaatattc tatgctacca agagcaacac aacaaagaaa gataagagat
    13681 gcatctaggg gaaaaattga tggaagaaca caagaaatac agaggttaat aggaagagca
    13741 ataaggtctg ttgtagactt gaataaatta ggagaaagaa ctatttgggt agattgtgat
    13801 gtgattcagg cagatggtgg aactagaact gcatctataa caggggcatt tgtagcagtt
    13861 gcagaagcaa tatataaatt atataaagca aagataataa aagagatgcc aataacaaat
    13921 tttgtgtctg caattagtgt tggtattgta aatggaatac acatgctaga tttatgttat
    13981 gaagaagatt ctaaagcaca ggtagatatg aatataataa tgactgataa gtgtgagttt
    14041 gtagaagtac aggggacagg agaagagaaa cctttttcga gagcagattt aaatttattg
    14101 ttagagttag gtgaaaaagg gaataaagaa ttaataaggc ttcaaagaga agctttaggt
    14161 gaaatatcag atgagatatt gggtatggat tatggtaatg aagtagtgat tgctactaat
    14221 aatgctcata agctagaaga aataggtgaa atcttaaaag attttgaata taaagtgtac
    14281 tctttaaaag atgttgattt agcaggtata gagatagtag aagatggaaa aacatttgag
    14341 cataatgcgt tgataaaagc tagaacaata gcaaaaaaga ctaaacttat agcaatatca
    14401 gatgattctg gcttagaagt agatgcttta ggcaaaaaac ctggtgttta ttcagctaga
    14461 tatgcaggag aacatgcaac agatgaagaa aatagaaaaa aactgttaaa agctatgcaa
    14521 aatataccaa tgagcaaaag aaatgcaaga ttcgtctctg caatagcagt agtttttcct
    14581 gatggaaaag aatttgtagt aagaggaatt tgtgaaggaa tgataggatt tgaagaaaaa
    14641 ggtaaaaatg gttttggata tgatagctta tttatagtaa aaggatatga taagactttt
    14701 ggtgaaatac ctagtgttat aaaaaattca atcagtcata gagcaaacgc actaaaactt
    14761 atgaaacaag agtttataaa aagggtagtg aagtaatgaa aataggcata gtaagcgaca
    14821 cacatatgat gattaaaaat atggaaaaaa caataccata tttgaaagaa tgtgacctaa
    14881 taattcatgc aggagataac tttactgatt caagatatat tcatagcatg actaatgtag
    14941 gtattattgc agttaaaggt aattgtgact ttgatgctgt tgaggaagaa gttgtctttg
    15001 aagtggcaaa taaaaccata ttcttatgcc atggagataa gtatggcgta aaatatggaa
    15061 ctcatatgtt agaaaaaaag gcaactgaag ttgatgctga tatagttata tttggccata
    15121 cccatacccc atttagagaa attaaagatg gtgtacttta cataaatcct ggaagcactt
    15181 cacttccaag aggagtatcg tataaaagtt ttgtcattat ggatatagaa gaagatgaca
    15241 taaaaattga agaaatacgc atataaaggt gtatttatct aaaaaaatgg caaaaaacgc
    15301 tcaaaaaatt aaattttatc aaataagtac aacgatatta atggaacact tgtaaataaa
    15361 aaaacttatg tgatataatt aagtggttga attataaata gaatgaatta taataccgtt
    15421 atcgggtatt acataaatat cactaggagg attagaagga tgaaagcaga actaattaaa
    15481 aaagagggta ataaagttac tttaaaaata acagtagata acaataaatt tgaagcagca
    15541 gtaaataagg cttataataa aagcagaaat aaatacaata tacctggatt tagaaaaggt
    15601 aaagctccaa gaattgttat agagactcaa tacggaaaag gtatattcta caatgatgct
    15661 atagaaatat tattcccaga agtttatcca gaagctatca aggaattaga catagaccca
    15721 atagacaatc cagatttaga tatagaagaa ataagtaaag acaatgggtt ggttatggtt
    15781 cttaatgtag aagttaaacc agagtttgaa ttaggaaact acaagggtat agaaatagct
    15841 aaggttgaga atactgtaag tgacgaaaat gttgatgcta aactacaaga aatggttgaa
    15901 aaaaatgcca gattagtaag tgtagaagac aaagctttag aagatggaga tacagctata
    15961 atagacttcg aaggttttga aaatggtgta gcttttgatg gtggtaaagg tgaaaattat
    16021 aacttagtta taggttcaaa cacatttata ccaggatttg aagaacaatt agtaggtaaa
    16081 aaagctggtg aagaagtaga agttaatgtt actttcccag aagaatatca ctcacaagat
    16141 ttagctggaa aaccagttgt atttaatgta aaaataaatg atgtaaaagt aaaagaatta
    16201 tctgcacttg atgatgaatt tgctaaagat actagtgaat ttgattcttt agatgaatta
    16261 aaagcagatg taagagctaa gttagaagaa gaagctaaaa atagagcaga tgcagaaact
    16321 agaaattcag tagtagaaaa agttgctgaa aatacagaaa tagaaatacc agaagttatg
    16381 atacaacatc aaatagataa tatgttaaat gaacttaact accaattaca atatcaagga
    16441 tttggattac aacaattact agagatgact ggtagaacta tggaagaatt aagagaagag
    16501 agaaaagaag atgctaaaaa attagttaag tcttctttag tattagaagc aataactaaa
    16561 gctgaaggaa ttgaagcaac agaagaagaa tttaaggctg agttagaaaa aatggcttct
    16621 gcttacaata tggaagtaga aaaaatagaa gcttctttaa gagatgccga caaagaagat
    16681 ataaaaggtc aaataaaaat aagaaaaaca atagatttat tagttgataa tgcaacaata
    16741 gcttaataaa ataaacgcta actaatatag ttgacgttat gaaatgttta gtatataatg
    16801 atgtgcaaat ataatttagt taaatgcgta taataattta cagtagctta aaaaaagcta
    16861 ctgtaaatta tatagaataa agggggtatg aaaccatggc attagtacct gtagttgttg
    16921 aacaaacagg aagaggagaa agatcttatg atattttttc tagactatta aaagacagaa
    16981 taatcttctt aggagatcaa gttaacgatg ccacagcagg gcttatagta gcacagctat
    17041 tatttttaga agctgaagac ccagacaaag atatacattt atatataaat tctccaggag
    17101 gaagtataac ttctggaatg gctatatatg atactatgca atacatcaaa ccagatgtaa
    17161 gtactatatg tataggtatg gctgcttcta tgggagcatt cttgttggca gcaggagcaa
    17221 aaggaaaaag actagcacta ccaaatagtg aaataatgat acatcaacca ttaggtggag
    17281 ctcaaggtca agcaacagat atagaaattc atgcaaagag aattttaaaa ataaaagaaa
    17341 ctttaaatga gatattatct gaaagaactg gtcagccatt agaaaaaatt aaaatggata
    17401 ctgaacgtga taacttcatg agtgcactag aagccaaaga atatggttta atagatgaag
    17461 tatttacaaa gagaccgtag atagagagag gtgttaatat gtctaaatat gaagaaaaac
    17521 gacaattaaa gtgttcattc tgcggtaaaa atcaagatca agtaagaaga ttaattgcag
    17581 gtccaaatgt atatatttgt gacgagtgtg ttgaactttg tgacgaaatt attcaagaag
    17641 agatagaaga tactatagat gaggatacta catctttacc aaaaccaaag gaaatgatgg
    17701 agattctaaa tgattatgta ataggtcaag aaaaagctaa aaaagcttta tcagttgcgg
    17761 tttataatca ttataagaga atatacagta aaaaatctag tagtaaggat atagaaattc
    17821 aaaagagtaa tatactttta ttaggaccta caggttctgg aaagacatta cttgctcaaa
    17881 ctctagcaag aactctaaat gtaccttttg caatggcaga tgctacatca ttgactgaag
    17941 ctggatatgt tggagaagac gttgaaaata tacttttaaa acttattcaa gctgctgatt
    18001 ttgatataga aaaagctgaa agaggtataa tatatataga tgagatagat aagattgcaa
    18061 gaaaatctga aaacccatct ataactagag atgttagtgg tgaaggtgtt caacaagcac
    18121 ttctaaaaat attagaaggt actgttgcaa atgttccacc acaaggagga agaaaacatc
    18181 ctcatcaaga atttttaaag atagatacta ctaatgtatt atttatatta ggtggagctt
    18241 ttgatggatt agagaagata attcaaaaac gtggtggaga taaaacacta ggttttggag
    18301 ctaaaataga aagtaaaaaa gaattagatt tagggaagct atatgaaaaa gtacttccag
    18361 aggatttatt aaaatatggt ataataccag agtttatagg aagaatacca gttttagcta
    18421 ctttagagtt gttggatgaa gatgcattaa tgcagatatt acaggaacct aaaaatgctc
    18481 tagtaaaaca gtataaaaag ttattggagt tagatgatgt tgagctagaa tttgaagaag
    18541 gtgctcttcg tgcaatcgct aaaaaggcca tagaaagaaa tacaggagct agaggactta
    18601 gaagtatagt tgaaagcgta atgatggaaa ctatgtttga agtaccatct agagataata
    18661 taaagaaggt tatagtaaca gagaaatctg tgaacgaaga ctcagtaaat cctatcatag
    18721 tacttaagga tcaagaggaa agtgcataag atgatgaaaa ggagctttaa aagctccttt
    18781 ttttatgtta atatgctaaa tataaaatag gtatcattat tataaaaatg tgagtaatat
    18841 acctagaata ccaaagacga atacaattgc aatggaacca atttttcctt tatataaaag
    18901 ataaaatgat gctataaatg caattattcc atataaatcc caattaactt ctttggcaga
    18961 tgaaaaagct gctgataata tgaatcctag agtaattggt ctaagaactg tcaataagtt
    19021 ttctaccttt ttattatttt taaatttaga aaataacttt gatataatag ttagacctat
    19081 taatgcaaaa attgtaacac caatagttgc ggctagagaa ccaaagaagc cagcagtctt
    19141 aaatcctaca aatgtagcac tatttattgc tacaggacct ggtgtagatt gtgatatagc
    19201 aagtaaatcc aaaaactctt gattagttat aaagtgatat ttagatataa attcttgctg
    19261 gataaatggt agcatggcat aaccgccacc aaaactaaat gcccctattt taaggaatat
    19321 taagaagagt agtattattt ctgacataga ctatatacac ctcctccaat tattaaataa
    19381 atagggctta tattgaatac accaacagat aaaactgcta taattaatag tagtatgtta
    19441 gttttagact ttggtagttt cttgaacatg ctcgtaaaag aatacaatat aagagctatt
    19501 acaacaggcg caactccttt aaaaaatcca tctaaaattt tagatgatga atttttaaag
    19561 tatagatacg ataagactaa gactatacaa aatgatggta aagcagagcc taaggtacat
    19621 ataagaactc ctggtatacc ataaagttta tatcctaata atacagatat attaactgcc
    19681 agtactccag gataactttg agctatggat aaataatcaa taaattcttc ctgctctaaa
    19741 agtttttgtt tgtttacaag ttcatcttgt ataagaggaa ccatagcgta tccaccacca
    19801 aatgtaaacg caccaatttt taaaaaggtt aaaaacaatt tcaacataat agaaacctcc
    19861 aaaagataaa taacatctaa gtatactaat attaatatta tatcataata aaaagataat
    19921 atagtatgat agtattctaa gaatttatca ttaatgttga aaattaactt gttcaaatat
    19981 ataatattaa aagcaaagca attagactag aaaaggagta atgttgatgg aacaaaatta
    20041 tactaaaata gatcatgaat taccattaat tcctctaaga ggattagcta tatttccata
    20101 tatgatatta aattttgata taggtagaga gatatctctt aaagctttag accaagctat
    20161 gatggatgaa gaacttatat ttttaacttc tcagaaagaa gcagaggtag atgagccagg
    20221 agaagaggat ttttatcatg tgggaacaat ctgtaaagtt aagcaaatga taaaattacc
    20281 aggagataca gttcgtgttt tggttgaagg tgtatcaagg ggtagagtaa agaaaataga
    20341 acaagaagat gggtatttca gagcagtaat agaagaaata gtatttgata gtgataattt
    20401 ggatagtgaa acagaagttg aaattgaagc atttgtaaga aatgtatttg atgcctttga
    20461 agagtatata aatattggaa atagggtatc accagaaata ttaatatctt tagctgatat
    20521 agaagatgta gatagattta tagatacgat agctgcaaac atatatctaa aatcaagcca
    20581 aaaacaagaa atattagaag aatttgatat tagaaaaaga cttgaactta tttattctat
    20641 attattagag gaaatagata tattaaaaat agaaaagaaa ataactttaa gagtaaaaaa
    20701 acagatgaat aaggttcaaa aagaatatta tcttagagaa caattaaaag ctatacagaa
    20761 agaattaggt gaagaagaag atataaactc tgaagcagat gagtatagag aaaagcttaa
    20821 aaaaataaaa gcacctaaga caactaaaga gaaaatagaa aaagagatag ataaattttc
    20881 aaaaatatct tctatgtctc cagatgtatc tgtaagtaga aactatttag atactatatt
    20941 ctcattgcca tggaataaag agacaaaaga taaattagat ataactaagg caaaagatat
    21001 attagatgaa gaccattatg gattagaaaa agttaaggag agaatactag agtatctagc
    21061 tataagaact cttgctaagt cacttaaagg gcctataata tgtctagttg gtcctccagg
    21121 aacaggaaag acatctatag taaaatcaat cgccagagca ttaaatagaa aatttgtaag
    21181 aatatctttg ggtggagtta gagatgaagc agaaataaga ggtcatagaa gaacttatgt
    21241 aggttcaatt ccaggaagaa taattaatgg ggtaaaagaa gcccaaacaa aaaatcctgt
    21301 attcttattt gatgagatag ataagatggc agcagattat aaaggtgacc cagcttcagc
    21361 aatgctagaa gtattagacc cagagcaaaa taaggatttt gtggaccatt acttagaaat
    21421 accttttgat ttatcaaaaa tactatttgt tacaacagca aatagtctaa gtaatatacc
    21481 aagaccatta ttagatagaa tggaagtaat agaagtatct ggatatatag aagaagaaaa
    21541 attaaatata gcaaaaaaat acctacttcc aaaacaaatt aaagaacatg ctttaaaaga
    21601 aaactttata aaaatagatg atgaaacttt aagaagtata atcaaccatt atacaagaga
    21661 agctggtgta agaacactag aaagaactat tggtaagatt tgtagaaagg ttgccaaaaa
    21721 atacgttgaa gacccaactc ttgaagaggt tgttataaat aagtctgacc tagagacata
    21781 tcttggaaaa gacatgttta agtatcaact tgcagaagtt aaccctcaaa taggattagt
    21841 aaatggtctt gcgtggacag aagttggtgg agttacactt gaagtagaag taaacgtact
    21901 taaaggtaaa ggcgaaattg tattaactgg aaaattagga gatgttatga aggaatctgc
    21961 gaagacaggt atctcttata taaggtcaat agtggacaag tttgatatag accctgaatt
    22021 ttataagacg aatgatattc atatacacat tccagaaggt gctgttccaa aagatggacc
    22081 atcagctggt ataactatgg ctcttgcagt aatctcagca cttactaaaa gacctgtgcc
    22141 tggaaatata gcaatgacag gagagataac tttaagaggt agagtcttag cagtaggagg
    22201 agttaaagaa aagttattag cagctcatag agctggtata acaaaagtat taattcctaa
    22261 agaatgtgaa gctgatttag atgaaatacc agaaaatgta aaagaaaaaa tggagtttgt
    22321 gcttgtagaa catatggatg aagtattaga acaagcttta ttaaagagtg gtgaaaataa
    22381 tgaaaattag aagttcagag ataactatga gtgctgtgaa taaatcacag tatccagcag
    22441 aaggaatccc agagatagca ttggctggta ggtcaaatgt tggaaaatca tcaataataa
    22501 atacattgct taacagaaga aactttgcta gaaccagtca aacaccagga aaaactagaa
    22561 ctataaattt ttatctaata aataatgaat tttattttgt agaccttcca ggctatggat
    22621 atgcaaagat agctaagtct gaaaaagaaa agtggggagg tataatggaa cgatatctag
    22681 aaagtagaca agagttatgt tctatattcc tacttgtaga tattagacat gagcctacag
    22741 cagatgataa gcttatgtat gaatggataa agcactttgg atataactgt gtagttattg
    22801 caactaaggc agataaaata tctagaggac aatatcaaaa acacataagt ataataagaa
    22861 aaaaattgca aatggaaagt agtgaaaaag taattcctgt ttcttcacta aaaaagactg
    22921 gtgttgaaga attatgggaa gagattgtaa atcaatacaa tcaacatgga tatgaaataa
    22981 cagtagatta aatttaaaat gttaaatatt gacataagat ttatttgaat aataaatgta
    23041 ataatagtta gttttaatta tattaatatt aaagtaaaat ataggatgcc ttaaaatagt
    23101 tttaatgaac ttttttttgg catccttatt gtttataaac aataaatatc agtaattttg
    23161 ctaaatacta ataaatttta gtaaggtatg ggaaaattag tataaaatag ttaaactaaa
    23221 aaattattga tttatgtagt cagtaataaa tagcatttaa gaaatagatt tatctgtttt
    23281 cctcataaag aaaacagtta gtataagccc tacaaaacat actgatgctg caatccaata
    23341 agactgattg ctagctataa cataggctga tgtgtgactt ttaaactttt gattgtatgt
    23401 aagactttgt gcaaaaaaac tactaaaaaa tgctgttcca agagcaaatg ataaattacg
    23461 aatagttgca agaaaactgc ctccgtaccc ctgtttttga ggaggaacat ctgctaaaat
    23521 actactattg tttggtgaac taaatgctcc tagcccaagt ccaaaaataa ctaaacatac
    23581 aattaaaagg tacaatggag aatctgcttt taaaagtgcc attccaacta gtgagatggt
    23641 cataaatgaa aaagatgctg ttagaatgtt ttttgcaccg tgtttatcag aaagtgaacc
    23701 tgcaataggt gctataagta ccataataag tggatgtaca gtcattacat aaccagccat
    23761 cataggagat aaatttttta attgttctaa ataaaatgga agtagaaaag atactgccat
    23821 ttgaggaaaa taacaagcta ctccaatgat attgcctaca gtaaagtttc tatttttaaa
    23881 tagagggagg tcaataaagc tttgttcaaa ttttacttct cttaatgcca acaataagaa
    23941 gattataagt gctgttatac ctaaatacaa tcttgtatta cccttctcat tcattgcaag
    24001 taatataagc gtagtgcaag atgcgaaaag tagaagacct ggaatatcaa agctcttgtt
    24061 ttgttcatcc aaaacaggtt taggcagaaa tttaatgcca agaaagactg ctataatacc
    24121 aatagggata ttgataataa aaatagaagg ccatccaaaa tgttctaaaa ttatacctcc
    24181 aattacagga ccactcatat ttcctatacc aactacagct ccagtgattc ctatagcttt
    24241 tcctttttca tttgctggaa atgcattgga aactatacca agcccagtag ccattaaaat
    24301 acttgctcca actgcctgaa agattcttga agaaattaat gttgataaat tgtttgacat
    24361 actacacaga aaagaaccta ttgtaaatat aaaaaatcct aatgtgtata gacgatttga
    24421 acctacttta tcgccaagtt ttccgaaaaa taacatagta gctgttacaa caagcatata
    24481 agctgttaca acccactgaa tttgtgacat gttaatttta aagtcagcag caattgtagg
    24541 atttgcgata tttagtatac ttccatcaag agtagacata aatacaccaa tacagatgat
    24601 aatcaatact atccattttt gtttcatgat aacactccaa tcttaataga ttttataagt
    24661 acgatataat tgtaaaataa acaacaaaaa aagcacctgt atattaggta ccttgaatgt
    24721 gtctaatttc atgaataaat acggtagttg aaggatttgt atagattact catgtaaaat
    24781 tacataaagg tactcatata ctattttctt ctaccatcca gattttactg tcggctttgg
    24841 aatatgacca aattatgcca taaggctcgc gggcttttac cgccgatagg taatttcacc
    24901 cgtttatgaa gaaaaacaga tgtattttgt aatattattt taacataaaa ttataatgga
    24961 aacaattgga tttaagtaat atccagctgt attacaatac aaatatttca atatggacac
    25021 ttaaattgta tttatagata gaataaggca cagtatgaga atctataata attagataaa
    25081 attaacctgt tattagtatt agatggcaag ttttaaattt gtcaaaatca gaaagctgta
    25141 ataaaattct tataatatga atatcttaat taagagatat tgtaaattat gaggaggcgc
    25201 taatatggaa ggaaaggtaa agaaggcatt cccgggtgga aatacatcac aaggctcata
    25261 ttcttttttt gactatataa ttcctgaaaa tgtaaaaaga gttttttgtt taaagggtgg
    25321 acctggagta ggaaaatcct cacttatgaa aaaaatttat aaagagtttt tgcatagagg
    25381 atatgatatt gatttatatc attgtccttc tgacccaagt tcgttggatg gtttagttat
    25441 aaagaaatta ggcgtggtac ttatggatgc aactgcccca catactatgg acccaaagct
    25501 acctggagct ttggatgaga taattaattt aggtgattac tggaatacag aagaattaga
    25561 aaagaataaa caagaaatat cagtttatga taaagatatt agtaattcat ttaaaagagc
    25621 atttaagttt ttaaaatcag cagaaccaat ctttagagac atagaagaaa aatattctga
    25681 ttgtatggat tatggcaaag taaatttggt cacagaagaa tttataaaaa ggttatttga
    25741 tggagttaaa agcacaggaa atctaaaaag agaaaagcat ttatttggtt cagctataac
    25801 tcctgtaggg tgtttagatt atacagaaaa cattttaaaa gatgtaaaga aagtctatta
    25861 tttagatgga gaaataggaa ctgggaaaac tacattatta aacaaagtat ataagaaagc
    25921 tattgaaaaa ggtttaaatg tagaagttta tcattatcca cttattccag agaaaattga
    25981 aactgtacta ttaactgatt tagatatagg cataactata agtacgactt ttaaagaaga
    26041 agatacaatt gatttaaatc agtttttaaa tagagaaaaa ctacttaaat atgaagaaga
    26101 tattaaattt gatgaaaaag ttttagatga tttaatttgt tgggcagtac taaatcttaa
    26161 gagagccaag tctaaacatg atattataga aagttactat agtcctaata tgagatttga
    26221 tgaagtaggg aaattaagag ataaattaat taaaagaatt ttaaaatatg aggaaaactt
    26281 ataaacgtat atgtttaaaa ttaatgttaa taatgatata atttttagta aattggtgta
    26341 gctttgaggc tatgccattt tacttttttt tgctaaaaaa tgatattaat aatctccaaa
    26401 gcaattattt gtttttcata attattattt cctttcctaa tttatgtttt tttataatat
    26461 caaaacaatg tttattgaaa gtttatataa ttttaagaac atctaaaaat gcatacttga
    26521 tattggtaaa ttagtgatag gagttcaatg tatgatatta tatactaaag atgaatttta
    26581 atatataact ttgatataat aagcatatgt aaatgtttaa tatttaatta aagaaaatta
    26641 tcatataaaa ataaatttgt aaggagcaat acagtatgaa aaaacaatat gtgtttataa
    26701 tactagcaag tactatttta ggtatacttg ttggaaccat atttagagat tatagcaatg
    26761 aagaaacagt ttatacaagc aaagacaagt caataagaaa agaaataaaa atgacaagaa
    26821 aatctataaa agaattaacc aaagagaaag acaagttaga taaagaaatt aattcaatga
    26881 aacaaaatca aaaaaattct gaagaaatat cagaaataga aacacttaga gatagtctat
    26941 catattcaga tgtaaagggt agtgggataa tattaagtat agatgctata gatgaaaaaa
    27001 cagggaatat agctaatagt atagactata ataagattct tgtaaaagtt ataaatgaat
    27061 taaaagtaaa cggaggaaaa tacatagaaa taaatggtca aagaattaat caatattcag
    27121 aagttgtact tgcaggaagc catataaata taaatggagt tcctgtagca caaccatatg
    27181 aaataaagtg cattggagat aaggagaaac tatctaatta tgtagataaa ggtagtaaat
    27241 atttagatag catacaaacc aattatccaa tgaaaataag cataaaaact gaagaaaata
    27301 tctctatgaa aaaaatgaat atacctaata aattaaagta tataaagggt gattaagatt
    27361 atattagagg aaagcagttt gccaatgtta ataccagaaa aacttgcaaa aaaatataat
    27421 gtatttccag ttgagtttca agatgataat ttagttgtag aaatagaaga tgaggatatt
    27481 tatgctctac aggacttgag attagctaca ggtaaagaaa ttatattgaa gaaggagaaa
    27541 aaagaactta taagtgaaaa gattgaaaaa tattataata gctttgaact tgatgaggat
    27601 tatgcaaaaa aattatttga aaatctttta gaaaaagcta taaaagaaaa cgcaagtgat
    27661 atacatatag aaccatttaa agaacattta atagtgagaa tgagggtaga tggagaacta
    27721 aaggaagtat caaagttttt agttgatgtc tatccatcat tgtctacggt tataaaatta
    27781 aaggcatcaa tggatataac agaaaaaagg cttcctcagg atggtagggt ggatataaag
    27841 ttaggtggaa atttaattga cattagagtt tcatcaatac caactgtcta tggtgaaaaa
    27901 atagttttga ggatacttaa tagagatact tttttaaaag acaaattaga acttggtttt
    27961 tcagaggaag ctataaaatc tataaatcgt ataataaata aaagagcagg aatattatta
    28021 gtgacaggtc ctacaggtag tggaaagact acaacagtat attccttatt aaatgattta
    28081 aaaggaataa ataaaaatat tatgactata gaaaatcctg tagagtataa gatggatggc
    28141 ataagtcaga ttcaagtaaa ttcaaaagta gggttgagct ttgatgtagg tcttagagcg
    28201 atattaagac aagacccaga tataataatg gtcggagaaa taagagatgc agaaacagct
    28261 aagatagcag taagagcagc tataacaggt catttagtta taagtacttt acatactaat
    28321 gatgcagtat cttccatagc tagattacta gaaatgcaaa taccaccata tcttttaaat
    28381 gcttctctta taggtgttat atctcaaaag ttggttagaa aagtctgcaa tcattgtagc
    28441 catgagatta tgataaaaga taattttagt ggagatgtaa atactaaggt agctgtaggg
    28501 tgtgaaaaat gtaatgataa aggatacttt ggaagaacag caatttatga aatcttagaa
    28561 attaatgatg atataaagac atgtataaga aatatggagg attctagtac tattaaagat
    28621 gtagcacaaa ataatggaat gataacattt gaagatagtt gtaaaagact aataaatgaa
    28681 aaaataacga cattagaaga gtgtttagta gtaaatgaaa tgatttaact gaattgtata
    28741 gttaaacttc gtttggtcag ttgtctttat ttaatctatt ttaattaaga tgtttgagta
    28801 taaattatat ttaagtgaaa taaaattaaa tgcattaaat tagaaaatat taagtttatt
    28861 taattgtaat taatataatt tggaaggtat tatagtacat gagattatct aaaaatatat
    28921 tttataattt agaaaagttt tatttagtat taaatagaat cagtgataaa gaaattagag
    28981 taatttgcaa agagataggc atattgctgg aatctggatg tgaaattaca aaaatatttg
    29041 aaatcattga gagtcagtca agtaaaaaag caaagaattt gcttagtata gtatcaaatc
    29101 atattcaaaa aggtaactca atagcagaat catttcaaat aacaggtata ttttctaaat
    29161 tctttataag tatgataaaa gcaggagaaa ctagtggaaa cttagatata ataatgagtg
    29221 atttatctaa ttattatgat aaagaataca aattaaaaat gaaaataata actatttcaa
    29281 tatatccaat aatacttata atattatcta tcttatcaat gttgtttata ttcgtatttg
    29341 taatacctaa ttttcaggtg gtttttacca acaatggaat tgaaccacct ttaataacta
    29401 gagttttaat gggaatatct acagtggtta caaataactt agcttatata atatttagtt
    29461 ttatattgtt ttcaatagga agcatttatt tctttataac aaatgataat ataaaaaaat
    29521 tgattaattc gttaaaattc aaaatacctt ttataaaaaa aattaatcag ttagtagcta
    29581 caacaagatt ttcaagggca ttgtccgttt taattagcag tggaatacaa attgtggaag
    29641 ctatagacat gtcagctagt gttatagaca acgaatttat ttatgaaagg ttaaagattt
    29701 ccaataatta tataaaaaaa ggaaatagca taggtaaatc tttgaattta gcaaatgttt
    29761 ttccaaaatt atttatttca atggcaaata caggagaaga aagtgggaaa ctagataaaa
    29821 gtctaaatgt aataaataaa ttttatgaaa atgaattaaa taacaaaata gagcaaataa
    29881 tgaaatttat agaacctatg ctaattgtat ttatgggatt aattattggt acttttattt
    29941 tagcaatggt tgttcctatg tttgatgcaa ttactaattt ttagagagaa aaataaattt
    30001 tattagatga aacttttgaa aaggagagaa tatttaattg ataaatttga taaataaaaa
    30061 acgaaaagga tttacacttg ttgaaatgat tgtagtagta actattttag gcgttatatc
    30121 tagtatagca ttagttaagt atagtaaggt tcaagaaagt gccaaattaa atgcagacta
    30181 tacgaatgct gctaatatag taaccgcagc tagcatggca attaatgatg atgaaaagac
    30241 aatagactct ctaagtgtag agacattgaa ggaaaaggga tacctaaata ctgttccagt
    30301 tcctcagagt acatcaggta aattcgaact tgtcataaat gatagcggaa cagatataag
    30361 cgtaaatata aattctaagc aattttatcc aaaataatat tttatattga ttgctgtaag
    30421 ttttaaaatt atttttattt aagaaggaga tggctataat taaaaaaatt ctaaacttag
    30481 aaataaatga tgattatata aggcttgtat ttttaagaaa aagtcataaa aaagttttta
    30541 tagataaatc aattataaaa gagattaact ttgatataaa aaatgatagt aattcgattg
    30601 agaagattaa atatatagaa gatgtagtta gatttgtaaa agaggagatt ggtaaatcaa
    30661 aaatattata taaaaattta tactttaaca ttcaaattca ggatatagtc attagaaatg
    30721 ttgaagttct aaatactaag aaaaaaagag acatattaag catgattgaa tttgaaataa
    30781 cacaatacat tccaataaac ataaataact attcaataaa atataaagtt ataaattcta
    30841 aaaatgataa attgaatata caagtaattc ttttcccaaa gagtattatt caattaatta
    30901 agaacatttc agaaaatcta aatatgaatc caaagactat aaatataaat tttgatatat
    30961 tacaaaagct tataaactta aatttaatag agaatttttc agaagaaggg gtatttatag
    31021 aatgtaaaaa aagtcagtta attataaata taacaaaaga taaaaaaata agagaaacat
    31081 acttgctatc aaaagaggga aaatcatatg agttcataaa aagattatta agggatttta
    31141 agtatgttta ttactatggt atagaaaatt gttttattga agaatacttt aaatatacag
    31201 ataaatctat tgaagtaaat ccattaagat taaaagataa agtaaaagta gaaaataatt
    31261 ttgatgcaga tttagaagac aataggataa attatataag taatatagga atgattattt
    31321 aagtgaaagt ggataaaacg ataaattttc ttaatggatt taaaaaaact aaaccatgta
    31381 ataaaatcta taaaataata atagtcttaa ttttaacgtg tattttattt aaatcattta
    31441 tgtatgtgaa atctataaat gaacttgaag attatataag tcaaaataag ctagtggata
    31501 aatcaagaca cactttacca acaacagata aaactagaga gaaattttct aaaataaaac
    31561 ttaatgaaat aaaaaaaata tatgaaacaa ttggaataaa tattattact aaaatatcga
    31621 tagataaaaa taagctagag attgaaggtg tgtgtagtga tttagatgtt ttaaacaatc
    31681 taaggaaaat aaagtacata aaatattatt acatagacag tattgtaaga gatggagaat
    31741 attatagatt tagaataaaa agttagatgg gtgattattt ttgagattaa ataatagaga
    31801 aaaaaatcta cttaaaatat tgttaatatt agttattata ttggcaagtg atatagcagt
    31861 aagttatttg gaagaaaact taactagttt gaagaatgaa aagcaaaata taataaataa
    31921 aataaataat caagataatt atcaaataaa ggataataag catatagatg ataaatatat
    31981 agaaagtgtt gaatcaaata aaaatgaaga attaaaaaat aattttaata actcattttt
    32041 agataaaaaa acaaataaag atatagtgtt attagtagaa aatgaactga gagatattgt
    32101 gaatgtagaa tatattaatc aagaaagtag tttagataat cacaataaaa aacaagttaa
    32161 tttagaaata aaagttaatg gagaacttgc aaatttgttg aaacttgaag gtgtaatgaa
    32221 gaagttaaga ctaaataata aacttgaaaa gatagaaatt aataaattaa aagtcaataa
    32281 ttttgatgtt caaaatattc caaatgaata taaacaagaa aaagacaata ttgttgaatg
    32341 taaaatgaca gttagagtca tataacaaaa aagaatagat aaagtaaaaa actattatca
    32401 attaataaaa ttttatagag gaattagttt tacgataatt catttctaca agagggattg
    32461 ttgatgattt tatcgaaatc ctaattaaag ttaatatatt gtaccaaaat ataagtgaat
    32521 ttaatagaat atttacttat actatagatt gattaataaa aaaatggtat aataacaaat
    32581 tgtactttaa tttaataata agggagtgga agtcatattg ttagggagaa atgagctatg
    32641 cccttgtgga agtggcaaaa aatacaagaa atgttgttta aataaagata ttgtttcaga
    32701 aagagcatgc agaaaaactg aactttcaca aaaacaatat acagatttat attcaaagtt
    32761 atatgattat tcaagacagg ataagtttaa agaagaatgt gaaaaagcta aagaaatgtt
    32821 ttatataatg caaaatgata gtgtaaatga aaaatttgaa agatttttta atacttattt
    32881 aatacaagac cacataatgg aaaataaaaa ggttatgaca gttggttttc ttgaagaaaa
    32941 tggtgcaaac ctaagtcaat cagaagtatc aatattaaaa agtatgtttg aatcatacgt
    33001 aagtatttat gaggtaaaag aagtttctgc tggaaaaata gtattaaagg actgtttaag
    33061 tggaaatgaa ctttgtacag aagatgtaaa acttctgaga agttttaaaa tcggaagttg
    33121 tatgatagct agaatagttg atattgaagg aactaacata ttgatagata taactataag
    33181 tatatcaaat gaggttaaag atataatttt aaaggatatt atgaatctat tcaatcaata
    33241 tcaagatgta tacaaagata tgaagtcgtt cttaatttat catacacata tactatacaa
    33301 gtatatacaa cagcttttag acccaagtat agctgaatat ttaaagagag aaagagataa
    33361 aaacgataca aaagttaatt cagaaaatga attagctgaa gaagaatgta aagttttaga
    33421 tactcttaaa gaaaatgtag aaaaagaaaa tatagatgca tgtatagagt tttggaatga
    33481 atataagaaa aatcatgaaa atataaaagg agctgaaaat ggttgggcag ctgctgttga
    33541 atactatgtt aaaaaagaag caggacaacc tataactcaa gttcaaatat caaagaaata
    33601 cgatataagc tctagtacac taggaaagag atacaaggat ttaaaaatat cttaattaat
    33661 tataaaaagc tttaagtcat agataaagaa tctatagtat aatatatgta taatgtttag
    33721 aaagaggtat taataatgag tcttagttta ggaaatgcaa ttgctgtatc aacgaacttt
    33781 ataaaatcta acccagctgt acctgtgtac aatggagaag aaatatatgc agttatttat
    33841 catatagaaa taggatataa taatggtgta tcacctgata ccaaacaata tataataata
    33901 gactataaag agtttgattt atctatggat aaaaaaaatg aactaataaa tattgctaaa
    33961 gagtactgta aatctaaagg tattgtagaa aacgaaaacc ttgaaataaa ccttttagac
    34021 aatgaagaag cagagaactt cttagaaaag gtgtttaata atatgaaggt tataagaggt
    34081 cttataacta aaaaaggata aataaatgga ctatatagga atagaaaata taacacctta
    34141 tgaaaataca tatgaattta gtgtatatga atatgatgat gaaatcacct taggtagtga
    34201 aaagttatat gtatgtgaat taagggttgt attgattaaa gttaattctc tgtatgttga
    34261 aagattgcat aaatcagttg aagcaatggt cttagtaaaa aacttgaaaa aagatttaga
    34321 taaaacactt gttgtaaaca aaataaagaa ttttgtgcta gatgagattt gggtagaaaa
    34381 tctagtaaaa gagaatatag aagttatatt tgtagaaagc taggaataac cctagctttt
    34441 ttatattaca atctaagaga ggaaaacaat atgttaagag tatctaatgt aaaacttgga
    34501 attgataaag atatatcttt attaaaagat gttatactta agaagctaaa aataaaagaa
    34561 aaaaacttag tcaaatatag tatatataaa gaatctatag atgctagaaa aaaaggaaaa
    34621 atggaatttg tttattctgt agatgtagaa gttaaagatg aaaataaaat acttaactca
    34681 aaaataaaag atgtaacaaa aataaaggaa atcagatacg taaatgtacc tgtgggtgat
    34741 aaaaaattaa agaataatcc tttggtaatt ggtagtggac cagctggttt gtttgcaggt
    34801 ctgcttttag ctcaaatggg ttatgaacca atcatacttg aaagaggtct tgatgtagac
    34861 agtagaacca aagatataag tgatttttgg acaactagaa aattaaaaaa taattcaaac
    34921 gttcaatttg gagaaggtgg agcaggaact ttttcggatg gtaaacttac aactagaata
    34981 aaagatatac gatgtagaaa agtattagaa gagttagttc agtttggggc accagatgaa
    35041 atattatact cacataagcc acatgttgga acagatatat taaaaaatgt agttaaaaat
    35101 ataagaaatg aaataataag tttaggtggt caagttagat ttaattcaaa agtaacagat
    35161 atagttgtat caaatgattc tatacaatct gtaataataa atgatactga aaaaatagat
    35221 acagatacaa ttatattagc tgttggacat agtgcaagag atacttatga aatgttgtat
    35281 tcaagaggta taaaaatagt tcaaaaacct tttgcaatag gagctagagt tgaacatcca
    35341 caagatttaa taaataaagc acagtacaag gatttttata atcaccctag gttaggagct
    35401 gcagattata gacttataga gcatgtttca aatggaagaa cagcatatac tttttgtatg
    35461 tgtccaggag gtagtgtcat agcatctgca tcaaatgatt ttgaaatagt tacaaatggt
    35521 atgagtgaac atgcaagaga taaaataaat gcaaatagtg catttttagt aaatgtaacg
    35581 ccagaagatt ttaagagtga taatccacta gcaggtgttc atttccagca gaagtatgaa
    35641 aaattagctt ttgaattagg tggaaaaaat tataatgcac cagttcagct tgttggagat
    35701 tttataaaca ataagacttc tttgaattta ggaagtgttg agccatcata tagaccagga
    35761 tatacactta caaatttaag tgaatgtttg cctagcttcg taacagagac tatgagagaa
    35821 gcgtttatat ctcttgataa aaaattgaat gggtttgcaa tgtatgatgc tgttttgacg
    35881 ggagtagaaa ctaggtcatc atcaccaata agaatagtga gagatgaaaa tactttagaa
    35941 tcagtgagta ttaagaatct atatccatgt ggtgaaggtg ctggatatgc aggtggtata
    36001 gttacagcag cagttgatgg tataaaatgt gcagaaaaaa taatacaaaa atacaccaat
    36061 aatacaaaaa tttaacataa acttaatgat tttttaatat ttaacatgaa tttaacatta
    36121 tcactcctat aatttgataa aatacaatag gttataaaat ttttagatac agtaatctct
    36181 ttacaaggag gaaataattt gaacattata ataagtctaa tgggtggatt aggtctgttc
    36241 ttatatggta tgaaccttat gggagaaggt cttcaaaaat ctgctgggac aaagctaaaa
    36301 aagataataa aactacttac tagtaaccta tttatgggag ttttggtagg tacaggagtt
    36361 acagcagtaa tacaaagttc aagtgctact actgtaatgg ttgttggttt cgtaaatgca
    36421 ggaataatga ctttaaaaca ggcaatagga gttataatgg gtgctaatat agggactact
    36481 gttacagccc aattggtatc tttcgactta actggtatgg ccccagtagc attaggtgta
    36541 ggtattatac tttatttatt tggaaataag cctagaataa aaaatatagc cgaaatactt
    36601 ataggttttg gtatattatt tacaggtatg gattttatga agatggcagt ggagccacta
    36661 agagactatc aaggatttac tgacttactt gttacatttg gtagatatcc actacttgga
    36721 ttattattgg gatttgggat tacagcaata atacaaagtt caagtgcatc tatgggtatg
    36781 ttggttgcac tggcagcaga aggtctagtg ccactatcag cagcactgcc aattctttat
    36841 ggccaaaata ttggtacatg tgtaacttca ctgttatcaa gtattggtgc taacaaaaat
    36901 gcacgaagag cagctatgat gcatctaatc ttcaatgttt taggaacggt gatattctta
    36961 atattcttga ataaaccagt agtaagcatg gttacaagtt gggatccaag caatgttgca
    37021 agacaaatag ccaatacaca tacattgttt aatataatat ctgtattgat acttttacca
    37081 tttacaagtc ttattataaa actagctata aagcttgttc ctgatagagc aggtgatata
    37141 gatgaagatg aaactaaaac tattaaatat atagatgata gaatgataga aactccttct
    37201 atagctcttg caaatacgat aaaagaagca cttcgtatgg gtgagaaggc gaaggaaagc
    37261 ctaaatgcat ctatggaagc tttagtagaa cattcaactg aaaaaataga taagacatat
    37321 agacgagaga gattaatcaa tgatttacag aaagctattt taaattattt acttaaactt
    37381 tcaaaagcag ctttagatga tgattcaaga gaagttgtgg atactttgtt taatacagtc
    37441 aatgatatag aaagaattgg tgaccacgct aaaaatatag ctgaattatc acaagttgca
    37501 atagattcaa atatttattt ctcagaagaa ggacaatctg aacttgatgt tatgtataat
    37561 agagttgttt cagcatatac ttatgcatta gaatcaatga ggagcgataa tgttgattta
    37621 gcttgcaagg ttataaaaat tgaagaacaa gttgatataa tggagaaatc ttgtagagct
    37681 aatcacatgt atagattgaa taataatcta tgtagtatag aaaatggagt tatttattta
    37741 gatgtaataa gtaatttaga gagaatatct gaccatgctg tcaatatagc acaacaagtt
    37801 atagcgaaga gattaggaaa tgattaaaaa taattaattt aaaataatat aaaatttaaa
    37861 atagaggata aataaaagag aaactttaaa tgatacgttt agagtttctt tttttgtaaa
    37921 tttatttgac taaaattttt aaatttatac atagttatag tatttagaag aatcgatttc
    37981 ctgataataa atgatgagat atgtatgatt ttgtggcaaa cttttaaaaa aaacacctat
    38041 attttgtgtt ataatctctt taaaacgaat atgctgaaaa gagggattta aatgggaaaa
    38101 ataaattttg attatagtaa agctacagag tttttttgtc aaaacgaaat agatgtcatg
    38161 caaccatatg tagatgttgc gcacgacatg ttacataata aaactggttt aggaaatact
    38221 tttttaggat ggatagacct tcctaaaaat tatgataaag aagagtttga cagaataaaa
    38281 aaatctgctg ataagataaa atcagactca gatgttcttt tggtaatagg tataggaggc
    38341 tcttatttag gttcgagagc tgctatagat atggtaagtc attcttttag gaatggactt
    38401 aaaaaagagc aaagaaaggc tccagaagta tattttgtag ggcataatat aagctcaaca
    38461 tatattatgg atttattaga tataattgaa ggaaaggata tttctgtaaa tgtaatatct
    38521 aaatcaggaa ctactacaga accagctctg gcttttagaa tatttaaaga ttacttagag
    38581 aaaaagtatg gtaaggaaga agcaagaaag agaatttatg caacaacaga tgcaagtaaa
    38641 ggtgcactta gacagttagc tacagaagaa ggatatgaga cttttgtaat accagatgat
    38701 gtaggtggtc gtttttctgt attgacagca gttggattac ttcctattgc tgctgctgga
    38761 cttgatattg atgctatgat gaaaggcgca aatgatgcta gagaagcatt tcaaaatcct
    38821 gacttaaaaa gtaatgatag ttatagatat gctgttgcta gaacaatttt acatagaaaa
    38881 ggaaaagatg tagagttatt agtgaactat gaacctcaac tacattatgt aagtgagtgg
    38941 tggaaacagc tttatggaga aagtgaagga aaagaaaata aaggtttatt ccctgcttct
    39001 gtagatttct caacagattt acattctatg ggtcaataca tacaagatgg aaaaaggctt
    39061 ttatttgaga cagtacttaa tgttgaaaac tgtaagagaa atataactat aagttcagag
    39121 gaagtagact tagatggttt aaattattta gcaggtaaga ctgtagattt tgtaaatcat
    39181 aaagcttttg aaggaactct attggctcat acagatggaa aagtacctaa tttagtaata
    39241 aatatacctc aactagatga gtataatttt ggatatttag tttatttctt tgagaaggct
    39301 tgtggaataa gtggatattt attgggagtt aatccatttg accaaccagg agtagaagct
    39361 tacaagaaaa atatgtttgc tttactaggc aaaccaggtt atgaaaaaga aaaggaagag
    39421 ctagaaaaaa gacttaaata attttaaata gttaaggttt aattaagatt aaaataatat
    39481 aatgaaatca tcaaaaagga gtttactcaa aatagatgat ttcatttttt ttatgaaaaa
    39541 ttgggaaaaa tatattttga taatttatat tatattaaga tagggagtgg tatgagatgt
    39601 caagaagaaa gaaagggata agtttagtaa tattagttgc tataataagt tcaatactta
    39661 gtagttttct aacaataatt ttagttaagg acaatttagt aagcaaatct acaggaagtt
    39721 ctacgccgat agttgtaaat gatgatggta agagtcaaaa tatttatcaa gcagttgcag
    39781 aaaaagctac gccatctgtt gtaggtatta caacaactag tgttgatact agtaatatgt
    39841 ttgcaatacc aactgaaaca caaggtgtag gaactggaat catagttgat tcaaatggat
    39901 atatacttac taattctcat gttatatcag atggtcaagc tacaagtgta aatgtattat
    39961 tcaatgatgg ttcaacaact tcaggtaaag tagtatggtt tgaccaacaa cttgatttag
    40021 ctatagttaa ggttgataaa actggactaa caccagcaga atttgctgat agtgacaaag
    40081 taaaagttgg agatatatca atagcaatag gtaatcctct tggattagat tttcaaaaaa
    40141 cagttactca agggataata agtggattag atagaactat acaaacagaa aaaactaata
    40201 tgactggttt attacaaact gatgcaagta taaatgcagg aaatagtgga ggaccattgt
    40261 taaatcaaaa aggtcaagta attggtataa acacagcaaa agcttctcaa gcagaaggat
    40321 taggatttgc aataccaatc aatacagcta agagtatagt tgaagaagta atcaaaaatg
    40381 gaaaatatga aaaagtaact ttaggcataa aaggtacaga tgtatcaaat tatgaagcag
    40441 caacaggtac aaaattaagt actgataaag gtgtttatgt tgcagaagta atatctggtt
    40501 catcagcaga aaaagctgga gtaaaggtag gggatattat aactaaagta ggagatacag
    40561 atataacagg tatgaatgat ttaaataaaa agttgtatac tttctcaaaa ggtgctagta
    40621 caaaaataac agtaaataga ggcggaaaag cagtaacaat aaatgtgaat ttctaaatat
    40681 gaccaaaaag atagagacta catggtaaag tgtagtctct atttttattt aatttttata
    40741 gtatatacat ctaaattaat ta
//
LOCUS       DAMKMI010000018        39834 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMKMI010000018 DAMKMI010000000
VERSION     DAMKMI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.018"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            177..1037
                     /locus_tag="PC429_000921"
     CDS             177..1037
                     /locus_tag="PC429_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3846439.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            complement(1276..1833)
                     /locus_tag="PC429_000922"
     CDS             complement(1276..1833)
                     /locus_tag="PC429_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3846440.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(2023..2910)
                     /locus_tag="PC429_000923"
     CDS             complement(2023..2910)
                     /locus_tag="PC429_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3846441.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            3029..4228
                     /locus_tag="PC429_000924"
     CDS             3029..4228
                     /locus_tag="PC429_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF3846442.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            complement(4422..6977)
                     /locus_tag="PC429_000925"
     CDS             complement(4422..6977)
                     /locus_tag="PC429_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3846443.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(6974..7660)
                     /locus_tag="PC429_000926"
     CDS             complement(6974..7660)
                     /locus_tag="PC429_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846444.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            complement(7737..8645)
                     /locus_tag="PC429_000927"
     CDS             complement(7737..8645)
                     /locus_tag="PC429_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3846445.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            complement(8647..9318)
                     /locus_tag="PC429_000928"
     CDS             complement(8647..9318)
                     /locus_tag="PC429_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846446.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            9580..10644
                     /locus_tag="PC429_000929"
     CDS             9580..10644
                     /locus_tag="PC429_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF3846447.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            10802..11488
                     /locus_tag="PC429_000930"
     CDS             10802..11488
                     /locus_tag="PC429_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846448.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYKDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            11478..12509
                     /locus_tag="PC429_000931"
     CDS             11478..12509
                     /locus_tag="PC429_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3846449.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            12591..13256
                     /locus_tag="PC429_000932"
     CDS             12591..13256
                     /locus_tag="PC429_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846450.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            13269..15686
                     /locus_tag="PC429_000933"
     CDS             13269..15686
                     /locus_tag="PC429_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846451.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSST
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(15867..16658)
                     /locus_tag="PC429_000934"
     CDS             complement(15867..16658)
                     /locus_tag="PC429_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3846452.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(16691..17278)
                     /locus_tag="PC429_000935"
     CDS             complement(16691..17278)
                     /locus_tag="PC429_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3846453.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(17455..18846)
                     /locus_tag="PC429_000936"
     CDS             complement(17455..18846)
                     /locus_tag="PC429_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF3846454.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(19036..20334)
                     /locus_tag="PC429_000937"
     CDS             complement(19036..20334)
                     /locus_tag="PC429_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF3846455.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGYPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(20691..21566)
                     /locus_tag="PC429_000938"
     CDS             complement(20691..21566)
                     /locus_tag="PC429_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF3846456.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(21759..22445)
                     /locus_tag="PC429_000939"
     CDS             complement(21759..22445)
                     /locus_tag="PC429_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF3846457.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(22494..23027)
                     /locus_tag="PC429_000940"
     CDS             complement(22494..23027)
                     /locus_tag="PC429_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF3846458.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(23071..23703)
                     /locus_tag="PC429_000941"
     CDS             complement(23071..23703)
                     /locus_tag="PC429_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF3846459.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(23793..24872)
                     /locus_tag="PC429_000942"
     CDS             complement(23793..24872)
                     /locus_tag="PC429_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF3846460.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(25005..26351)
                     /gene="pepV"
                     /locus_tag="PC429_000943"
     CDS             complement(25005..26351)
                     /gene="pepV"
                     /locus_tag="PC429_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF3846461.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(26420..27700)
                     /locus_tag="PC429_000944"
     CDS             complement(26420..27700)
                     /locus_tag="PC429_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF3846462.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(27827..28087)
                     /gene="hfq"
                     /locus_tag="PC429_000945"
     CDS             complement(27827..28087)
                     /gene="hfq"
                     /locus_tag="PC429_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF3846463.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(28201..29136)
                     /gene="miaA"
                     /locus_tag="PC429_000946"
     CDS             complement(28201..29136)
                     /gene="miaA"
                     /locus_tag="PC429_000946"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF3846464.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(29174..31141)
                     /gene="mutL"
                     /locus_tag="PC429_000947"
     CDS             complement(29174..31141)
                     /gene="mutL"
                     /locus_tag="PC429_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF3846465.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(31152..33995)
                     /gene="mutS"
                     /locus_tag="PC429_000948"
     CDS             complement(31152..33995)
                     /gene="mutS"
                     /locus_tag="PC429_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF3846466.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(34156..34890)
                     /locus_tag="PC429_000949"
     CDS             complement(34156..34890)
                     /locus_tag="PC429_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846467.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(34859..35872)
                     /locus_tag="PC429_000950"
     CDS             complement(34859..35872)
                     /locus_tag="PC429_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3846468.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(35894..36664)
                     /locus_tag="PC429_000951"
     CDS             complement(35894..36664)
                     /locus_tag="PC429_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846469.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(36639..36929)
                     /locus_tag="PC429_000952"
     CDS             complement(36639..36929)
                     /locus_tag="PC429_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF3846470.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(37060..37159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      37779..37868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            37921..38097
                     /locus_tag="PC429_000953"
     CDS             37921..38097
                     /locus_tag="PC429_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846471.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(38212..38568)
                     /locus_tag="PC429_000954"
     CDS             complement(38212..38568)
                     /locus_tag="PC429_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3846472.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            38824..39737
                     /locus_tag="PC429_000955"
                     /pseudo
     CDS             38824..39737
                     /locus_tag="PC429_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT        13552 a         6196 c         4634 g        15452 t
ORIGIN      
        1 aaaacttcat aggagagtaa caacatcagt aaaaatatga aaacactata taaaaattca
       61 aaaatattaa gaataatgaa atacttgaaa ttgcttatat caagaaattt tatataatat
      121 aaactggtca aatatgttat aatattaaat tgtgacaagt tcatggaggt gtttatatga
      181 aaaaacaaaa gtcgccaatt ttacttgaaa tgcttggact attttttggt tgtatatcaa
      241 tgagtattgg tataaatatg tttttaaaac cacatacaat tgctcctgga ggacttagtg
      301 gtctatcatt agtactaaac aaggttacag gacttccagt atcagctatc atgttaatta
      361 taggtgtccc attagtaata cttgctttta gaattatggg tacaaaaaat tcattaaaaa
      421 ctttatttgg aacagttgta ttttcagcta tagttcaatt aacagaccca ttatcaaaac
      481 taagatttac taatgactta ttattgtctt ctattgcagg aggaatccta gttggtatag
      541 gtttaggaat aatgtttaaa tcagatgctt ctactggagg aacagactta attgcactca
      601 tacttagtaa gaaattccca ggaataaaag ctacaaaatt catgtcatgc ctagatggaa
      661 tggttgttat atcttctggt atagtaaatc gtagtttaga aactgctctt tactctggta
      721 tagcactttg tgtattaata aaaatagcag atatgataat ggaaggattt gaccattcaa
      781 aagcattttt tattatctct gatgagcctg aaagtttaag acaagctata actgaagaac
      841 ttgatagagg tcttactatt cttgatggta aaggtggata cactagagaa aataaagaag
      901 ttctattagt cgtagtttct aaaaaacaag aactttatct aaagagatta gttaaaagaa
      961 ctgaccctac tgcatttgtt attgttacag atgtacatga ggttcttgga gaaggattta
     1021 aaaatttaga gaactaataa aaattaatat tttaagtata tatactattt taattaaaat
     1081 acgatttatt caaaaagagt gtctcataaa ctttttagtt catgagacac tcttttttgt
     1141 ataaaatcaa atgcattttc agtattttaa caataaaaac aggattatct atattttgag
     1201 gcaaccactt tattgaaatt ttatttagaa aacaatcatt actaaataac cagcatagat
     1261 attaaatgat tatatctatt gttttttcaa ttcatattga taacaaggaa catttttcac
     1321 agtatttcca gtatgaattt ttttgatata ctttcctgta catttaaatc ctaataaatc
     1381 taatgatttt gtagaatttt ggttttcttc ataggttatt gcaattattt tatttaagtt
     1441 tagttctcta aatccatact gtattactct ttttgttatc tcagtagcaa aatcatgtcc
     1501 ccaatagctt ggatttatac atatacctat ttctgcttct aatgctttag gatttatatt
     1561 attaaacccc atattcccaa taactttatt gtctctttta tatactatag caaagtggcc
     1621 aattttccct tttttatatg gatttagaaa aaatgatttt ataaactttc ttgtatcttc
     1681 taaagattta tgctcactaa atggtaaatg ttttactact attttatttt tataacagtc
     1741 aaaaaaatct tcaacatcat ctaatgatgc aggtcttagt atacaatttc tagtttctaa
     1801 ggttttcatt tgaggatatt cagaatcaga catttctaat ccccctttat acattaatct
     1861 aaatatactg ttcatattta aaatacgata ttaattatat aaatgttctc aaattaacct
     1921 tatttttatt tttaataaaa actaattact atatgaatct ttcaaattta cgaatttatc
     1981 tctattttta tattaaatta cttgtgaaat atgtattcta aattatatat tattagatat
     2041 catgcttaaa aattctttgg ttgctaagga taaagatata tcttttaaat ggcagtaacc
     2101 tatatttctc ttaggtattg gctcttttat atctaattta aacaatttct cattttccaa
     2161 ataatctatg ctaaattctt ctataacaca agacactcca agatttatat acgcaaattc
     2221 taaaagtaat tcatgagaac ccaattctat atccgggttt agttttatac cttttgataa
     2281 aaaatattca tttacataaa gtcttgaatt tgctttattt tctagcatta ttaaaggcaa
     2341 tgtgtttaat tcttccaatg atattttcct acctttgtat tctatgtaat catttgcaca
     2401 tacaaatata tcatgtattt caatacactc aacaatattt agtgtatcat cttctattgg
     2461 catgtttata attgctatat ctatattccc atctttgagt aaagatataa gttctctact
     2521 cgttctattt attgtagata catgtatatg tggatacttc ttatgaaaaa tttctaaata
     2581 ttttaacaag aaaaaacgtg cagctgtatc tcctactcct attttcagag aaccgtattc
     2641 taatttttta aatttattta ttctatcttc tcctgatgat attaacttta atgcagtagt
     2701 gacatgttca cttagaattt tcccctctgg agttaatttt actcccttat tgtttctata
     2761 aaataataaa gtatctaatt gttcttctaa ttgctttatt gactggctta cagctggctg
     2821 tgacataaac aattccttag cagcaagaga aaaactctta ttatttacaa ctgcattaaa
     2881 aaccttataa agttcaagtt ttatattcat atataattac tccttatgtc aaatattagt
     2941 attatttatt ttacttataa ttatatttga attataatat acatggtact aatatgacaa
     3001 tattaattct ttaggaggat tttagataat ggatagatta gttggaactg tttcaagagg
     3061 ggttagagca cctattataa gacaaggaga tgacttagtt aaaatagttg ttgattctgt
     3121 tttaaatgcg tcaaaaagtg aaaactttga agttagggac aaagatgtaa ttgccgttac
     3181 agaagctgtt gtagctagag cacagggaaa ttatgctcac gttgacaata ttgcaaaaga
     3241 tgtaaaagat aaatttggag atgatactgt tggggttata ttcccaattt taagtagaaa
     3301 tcgttttgct atatgtttaa aaggaattgc aaaaggatgt agaaaagtcg tattaatgtt
     3361 aagctaccct tctgatgaag ttggaaatca cttaataagt attgatgaac ttgatgataa
     3421 gggaataaat ccttggtcag atgttttaac agaagaaaaa tacagagaac tatttggata
     3481 taacaagcat acatttactg gtgtagatta tgttgattat tataagaatt taatcaagga
     3541 ttgtggtgca gaggtagaaa tagtatttgc aaacaatcca aaaactattc ttaactatac
     3601 aacaagtatt ttaacttgtg atatacatac aagacaaaga acaaagagaa ttttaagaca
     3661 aaatggagct aaaaaagtat attcacttga tgatataatg acagcaagtg tagatggtag
     3721 tggttacaat gaccagtatg gacttttagg ttcaaacaaa gctacagaag aaacagtaaa
     3781 attattccct ataaattgtg atgaagttgt aaacaaaatc caaggtaata taaaagaaat
     3841 aacagggaaa gatgtagaag taatggttta tggagatgga gcttttaaag accctgtagg
     3901 taaaatatgg gaattagctg acccagtagt atctcctgct tatacaaaag ggctagaggg
     3961 aactccaaac gaagtaaaat taaaatactt agcagataat gattttgctg acttatctgg
     4021 tgatgagtta aaagaagcaa tttcaaaata catagttgaa aaagataata aatctgatga
     4081 tttaacagga aacatggttt ctcaaggtac tactccaaga agattaacag accttatagg
     4141 ttctttagca gatttaacat ctggaagtgg agataagggt actcctatta tttacataca
     4201 aggatatttc gataactata caaaatagta tgttttaata agatttaatt tttttggatt
     4261 atatatatta acatgaaaat ttattaaaac ctcttaatag atatgacttt tcaagagtta
     4321 tatctattaa gaggtttatt taatgttcac agataagata tatttaaata atttaaacaa
     4381 gattgtcaga aagttatata atcttaaaaa tttatataac tctaattctt atttatttct
     4441 gaaataatat cactcttcaa aatttgtctt actggaataa ttacagcaac tatactaata
     4501 gtgatattta aaataatcat acctatgtat agaggtgtat ttattttaag aattggattt
     4561 atatataaat atacacgttt taccataaaa taaatcataa attgtccaat aacacttcca
     4621 attaccatta taatacttgc atagaatcca tataaaaaac cttctcttat catcataagt
     4681 aaaaacttat tatccgtaat acccatagct cttaaaattc caaactcatg tcttcttgaa
     4741 aaaattaagt agcttactgt attgattata tggaacaaac ttattgcaag aagtacaaat
     4801 acaataccat atataaataa gagtttttga tttaagtagg tattattttt ttcaatagca
     4861 atggtataat ctgttacgat acatcttttt acatcactta caacactttt tatgttctca
     4921 gacaattcct cactgttact atcttctgtc ttttttatac ttactgaatt ataattttcg
     4981 atatcaaagt tatttttcat ttgcccattc gtcataacta tatccatacc ataatcacca
     5041 atgaaatata tgttatttgc catcacacgt tttacagtag cagcaactac aaattctttt
     5101 gttgtataat cattcttatt ttcttcttta aatttaacaa tttcatctgt tggtttcatt
     5161 gtctttggca ctttaacttt tattgtatct cctggattta tatcaactgc atcatacatc
     5221 ccagttccat ccataggtag tctaactata accttattat ttttaaccat atcatgggta
     5281 ttaatctccc catctataat ataagaattt aatccttcta acataccatt gttataacca
     5341 taaatatttg tttttaaaag atagccacct tcattttgct ttgtacaaat accatcaaaa
     5401 aaagtcttta tcctgtaatc tttttcaaga ggtttaaaga aatttttcca aaatagttgt
     5461 tcttctttaa tttgcatagc tccaaataaa tatcttactg gatagacttt tgatattccc
     5521 tcaacattgt ttaatcttga aattttatct tttggtatac ctacatcaaa atctgttgtt
     5581 tgcatagtta tttggtagtc agaatttaaa ccatcatcag ctttcattgt aagttcatta
     5641 ttatttttag taagctgtat agagtaacta gaacatagaa atataattcc accaagtgat
     5701 aatgaaaaca aaattccaat aaacattccc tttttttgag tcatgtattt atgagataat
     5761 acttttgtca tgttagagtg ttttacactt tctaatattc tctttttctt attagtaatc
     5821 ttaaagtttt tcatagattc tatactagag tatttattca actgatgtac aacacgtaca
     5881 gcaattaaaa ttaacaacaa taagataaat aaaaagccta aataaataga tttttcactt
     5941 ataaaaaatg ctcctggaac aatatcacta ctcaaaaata tatgtggaaa ttgttcatat
     6001 attgtagacg ctactccaat acctaagaag caacctatag gaaatccaac aataaaaagt
     6061 aaaaataact ctaaaaatag tagaaataaa agttgtttat tattagcgcc taatacttct
     6121 aaaaccccat attgtgatat tctctgaagt attgaaacat ggaaaatact atatactgct
     6181 gctgcaccaa acactccaac aagcaatata attgcatttg tatttagttc taaatttttt
     6241 aagaactcaa atatattata tttttctata ttttttggtg gttttactcc aaattcaact
     6301 gaaagagacc agtttatata agttttttct tttgatatgt tgaatttttt cataaattta
     6361 taattttgct ttttaatatt tttattttca tcatatttta cataaactat ataagaatcc
     6421 ttttctttag gctgaaaata atcgtttaca aatccctcca gagcagtttc tgggtttgaa
     6481 agacttgata aaataccaca taacttatag attttaccat ctaaattgat ttctgttcca
     6541 atttctttgt ttacatttaa attgtttaga gaatatgtgt caagtactat ctctccatct
     6601 ttactaggaa gagttccttt taataatttt ctacctgtca tgtctaaata tgaagaatca
     6661 gcatttaaaa attctataat aaaaggttca tctatctgat ttttaggttt tgtaacgcca
     6721 agtctatcta cagtgtattc ctttgatttt ttattttctt taattttatt aagttgttcc
     6781 ttatctactt tatagaaata atgataatct ccactatctt cccttatctt ttcaattcta
     6841 cttttgtctg cactatgaac taaagaacca attcccgtca ataatgctac tgatagtact
     6901 atgcttaaaa ctatagatat agattgtttt ttataatgct ttatatattg aattgaaaat
     6961 tttactagag acatcatagt aattcaccac cagtattaac cacaactcca tcttcaatat
     7021 ggataattga gtcgcatact tgtgctatat tttcattgtg tgttaccata agtatcgttt
     7081 gatggaattt tgcactactt tcttttaata aacataccac ttccattgta gttttactat
     7141 cgagatttcc tgttggttca tctgctaata taagcgcagg tttatttgcc aaagctctag
     7201 caattgctac cctctgttgt tgaccaccag atagttcgct tggaaatttt aaacgcttat
     7261 cccaaattcc caatgtcttc atcagcattt caatatattc atggtctata tggtttcctt
     7321 tatctaatgt aacaggaaga gctatattat cataagtgtt taatacgggc attaaactgt
     7381 agttttgaaa tataaatcct atatttcttc ttctaaacac tgtcaaatct ttttttccta
     7441 ttttagatat gttctttcct ttaattatta catcacctga ggttggatta tctaatccac
     7501 ctataatatt taacaatgtg ctttttccag aaccagaagt tccaactata gcagtaaatg
     7561 tatctcgttc aatttctata cttactccat ttaaggcttt tactttagtc tcaccttttc
     7621 catagtgttt tactaaatta ttcgtcacta atatactcat ttaatttctc ctttcaatac
     7681 agatatatgt tctataaaat attgtaagtg acgataatta cactcatcct acaaaattag
     7741 tttaaactct tacaattctg taagaagata ctaaaacaac atccacttcc tagtttagat
     7801 tctactataa tgtttccatt ttctttttct aaaattaatc ttgtaagata tagaccaatc
     7861 cctgtgccat tttggttttc aacattttta cttctgtaaa atcttttaaa aatatcattt
     7921 agttctctac tatcaatacc tattccctca tctttaatag taatcttcgt atatagttcc
     7981 atttttatta cagaaatagt aatcgtgcta tctgatggtg agtattttat tgcattttct
     8041 aaaacattta caattgcttc tatggtccac tttttattat gaactaattt tatgtctgaa
     8101 aatggttcga gatttatcct tatattttta gcttctgctt tattaaagat agtactgata
     8161 ctttgaataa gagtatcttt aattagtagt tcttctactt caaattctat aactccatcc
     8221 tctaatttag tcattttaaa taaggattgt aacagccagt caatcttaaa agtttcattt
     8281 ttcatctttt gtaaaaactc cttcttctgt aatgtggaaa tatttatatt ctccagaagt
     8341 tcacaataaa tggttatatt tgaaagtgga gtttttaatt gatgtgacat atttgatatt
     8401 aaacctttaa tcgcttcttt ttctgacttt gactgttcta cctctatttc tatcatatcc
     8461 ttaatttttt ttacttggtg agaaattttt gacgctcttg tatcctttgt atctggataa
     8521 gtaacttctt taccttccaa tatttgattt aacatcaaat ttaattcatc atatcttttt
     8581 cgatttattt tagtagatac tataaatata acaactgtac atattataaa ggctaatata
     8641 aacatattat tctacctcat tccataaata acccattcca taagctgttt ttatatattt
     8701 tggattagat gggtcatcct ctacttttac tctaagtctt cttatattta cagatacaat
     8761 attatcatca acataatttt cttcgctgtc ccaaatatgg tgtaaaattt gttcctttaa
     8821 taatatttga cctttatttt caattagata acttagaagc ttatattcta cagagctaag
     8881 ttccaaacat tctttatttt tatatacttt tagtaacttt tggtccaatt ttattccatt
     8941 ggaatatatt actttattat tcgatttttt tcttattgct actttgactc ttgcctttaa
     9001 tacagataaa gaaaatggtt ttgtaatata atcatcaagc cctatttcta atccttttac
     9061 ttcatcaatt tctgagtctc ttgcagtaag cattataatt ggtatatctg acttttccct
     9121 tattatttga catacatcaa atccatctcc atctggaaga ttgcagtcta agattataaa
     9181 atctatattt ttactattaa atatatttaa tccttcttgt tttgtataag ccttaaatac
     9241 attaaatcca tcattctcaa atgcataaaa aagaccttca tttaagttta tatcatcttc
     9301 aataattaat aagttcaatt ttactctcct ttagattttt tagataaata attattttct
     9361 tgcattatca tactaaaaat attttaattt tatttatttc actaaaaaga ttgtatttaa
     9421 atttgttttc aaaataaaaa cattataatc aagcatatag ttatatattt cccaatttaa
     9481 ttacgcttaa attgaaaata taactttatt cttatataat taaattatac aacaataaag
     9541 ttatattttt taaatatatt tttactaggg ggaatagaat tgaaaagggt tgatgagaga
     9601 aatactatgt ttgcaagagc aaattacaaa aaagatagta ctgcttataa tgactattat
     9661 aagaaaaatc ctgacaaaaa agaaattgat gatagtataa gaaatagacc aaatttatgt
     9721 agtgaaggaa caatgactta caatgagtta aactctccaa tggcaagtag tgcattcgat
     9781 tttttatctg atatcaaatc tttatgcgaa ggaaaagttt ctgatactaa agtagatgta
     9841 gatgcaaagt caatgacaaa aaaaattaag ggtcttgcaa agcagtatgg agcttctgta
     9901 gttggtataa ctaaattaaa agactatcat atttatacaa ataggggtag acatgaagaa
     9961 aattacggtg aagaaattaa tctaactcat aagtatgcaa ttgtatttgg atgtgagatg
    10021 gataaagaaa tgataaatag agctccaatg atatgtgaag ttatagaaac atcaaagtgc
    10081 tatgtggatg catctatcgt cggcatgatt ttaagttact atataagaaa tttaggatat
    10141 gatgctagaa atcatatgga tgctaattat ttagtaatgc ctgtttttat agctcgtgat
    10201 gctggtttag gagatatagg aagaaatgct atacttacaa ataaagatta tggctctaga
    10261 cttagattag gtgttgttac tacagatatt cctcttttag aagatgaata tgttgatttt
    10321 ggactagaag atttttgtaa ggtctgtaaa aaatgctctt ttaactgccc ttctcattcg
    10381 ctttctaatg atattaaaat tggtgatgat ggaaaatata attgggtaat tgaacatgag
    10441 acatgctata ttaaatggag atatttaggt acagattgtg gtatgtgtat atctgcttgc
    10501 ccatttagcc aaaatctcga aactataaag aatactactt catttaaggg taataatgag
    10561 cttattcaaa aagctttaga tgagtatact tcaaaatttg gtaaaagaat atttatacct
    10621 ggaaatccat cttggttgaa ataattttat ttaataaaga atctgtacta gaatttatca
    10681 taggacaggt tctttattaa atatataaat ttttaaatac tttttctcgt ttataacaat
    10741 tcagcaatgt ttttagtcta taattgacat tataaaatat taaactaaaa ggagtaactg
    10801 tatggaaaaa atattagtag tagaagatga ttctatatta aataaaactc tatcatataa
    10861 cctaattgaa gatggatata taattacttc aaaatttaca gctaaatctg cactaaagtc
    10921 tatttttgaa tgtgaatttg atttaataat tttggatatt aatttaccag ataaaagtgg
    10981 gtttgaatta tgtaatgaaa taaaggaaaa ttacaatata ccaataattt ttttaactgc
    11041 taatgatatg gaatgtgata tgattaaagg ctatgaactt ggagctttgg attatataac
    11101 aaaaccattt aatatcaata tatttaaaca aaaaattaaa gcttttttaa atcatcttac
    11161 aataaaaaca aaacaagatt actataagga tggatatctt gaaattaact tttctgagtt
    11221 atctgccaat ataaatggaa atcaaattat atttacacca ttagaatatc gtactttaaa
    11281 actgcttact gaaaatccta aaagtatttt aacaaggaaa gtacttttag aaaaactctg
    11341 ggatatagat gctaactttg tagatgaaca tactttaaca tctgttataa gtaggattag
    11401 aagcaaaatt gaaaaagaca atcttaagta tatcaaaacg gtatatggta tgggatatat
    11461 gtggttaggt gaaagaaatg aattttaaaa aattttcttt tagttctatt tatttaataa
    11521 ttgctgttgg tataattttt acagctattt tatttagtat ctcaatttat ctaataactg
    11581 gtgatttaaa actaattgct ctattattaa tatatacaat ggtactttgt ggatttatag
    11641 cattaataat atttttactt cataaaaaaa tagttatatt ttgttctaaa atatgtcaca
    11701 ctttagatag tatgatgaat agtgaagata atattgaaat tgaattagaa gaagaaacta
    11761 ttttatcacg tatcaatcac agattaatta gaatgtatga agttattcaa gaaaatcgca
    11821 atagtattgc tatagagaaa gctgatttac aagaactagt ttctgatata tctcatcaaa
    11881 ttaaaactcc tattgcaaat ttaaaaatga ttaatactac tctactaaat caaacattta
    11941 aaacagaaat tcaaaaggat tttttaatgg atatggaaaa ccaattagat aaattagatt
    12001 ttttaatgca atctatggta aaaacctcca gactagagac aggtataatc acactatcta
    12061 aaaagaaaaa ttcaatttat gaaacaattg ctacagcact tagtggaatt ttgtttgatg
    12121 ctgaaaaaaa gaatattgaa gttacagttg attgtgaccc aaatttatat ataattcatg
    12181 acaagaaatg gacttctgaa gctattttta atatattaga taatgctgta aaatacacaa
    12241 gttctaatgg taagatacgt gtagctacag aatgttgggt tatgtataca aaaatagata
    12301 ttattgataa tggaaaagga atatcagaaa gtcgccaagc agaaatcttt aaaagatttt
    12361 atcgtgaaga agatgtccat gacatagaag gtattggaat aggtctctat ctttcaagaa
    12421 aaattatatc tcttcaaggt ggctatatta aagttacatc agatattgga aaagggtcga
    12481 ctttttctat tttcttacct aataaataaa tttttaaaaa tttcatttgt aatgattctg
    12541 caacattttg gttttatact gtatttacaa gatgttaagg aggtattatt atgagtattt
    12601 tacaagtaat taatttaaag aaatattatg gacaaagttc aaacattaca aaagctcttg
    12661 atggtgtaac tctatctatt gaaaaaggcg aatttatttc tattgtaggt acatctggta
    12721 gtggcaaatc cacattatta aatatgatgg gaggattaga taatcctaca tctggaaagg
    12781 ttattataaa gggtaaggaa ttatctacta tgtctgatga acagcttaca atctttagac
    12841 gcagaaatat aggttttata tttcaaaatt ataatcttgt tcctttactt aatgtatatg
    12901 aaaatattat cttaccaata ggattggatg gagagaccat agataaggat tttataaatg
    12961 aaattataaa tatgctttct cttgaagaaa aattatatga tataccaagt aatctttctg
    13021 gtggtcaaca gcaacgtgtt gctatagcta gagcacttgt aacaaaacca gctattatac
    13081 tagctgatga accaacaggt aaccttgata gtagaactag ttcagatgta ctgagtttac
    13141 ttaaagtgac aagtaaaaaa ttcaaccaaa caattgtaat gattacacac aataatgaaa
    13201 ttgcacaact aactgataga attattcgta ttgaagatgg aaaaattgtt aactaggagg
    13261 tgtagtatat gtttagaaat aataatcgca catttataag aaaaatagct ttaaatgatt
    13321 taaaaattaa taaacttaaa acatatcttt caggtatcat tataatgatt tctacatgtt
    13381 tattattaac agtgacctta gtttcatata atgcctctgt agatttagtt aatgcatctc
    13441 catatcatgc tatatataaa tctgtagatg aaaaagcaaa aaatatacta tataaaaata
    13501 aaaactttag tggcgtagga acttacaaat taattggaag taataaaaag acagattgta
    13561 ttatgtcgat tgtttatgca gatgatacag caattaaact tatgaatttt caaccattga
    13621 aaggcaaact acctacaaat aaaaatgaaa ttgctatttc agaaaaatat ttacaggaat
    13681 ttggtttaga taaagatatt ggagatagca ttaaattaaa ttattatgat gatattacga
    13741 ataaagaagt tcaatgtgat tttattattg ttggatttct agaaaattat tatcaagata
    13801 atgccaagca atactataca gtcgtttcaa atgattattt taagaatata gcttctacac
    13861 ctttacaaaa taaaagtaat acttttgacg agtcaagacc tgatactgta gatgtattag
    13921 ttaaactatc agaagaaaat acagagaaag attcagcttc aattaagaca cagttaaata
    13981 aaattggttt atctttaggt attaagaaat ataatatata cttaaatgat aactatattg
    14041 aatcaaattt aattgatggt gagcagatta ttacagtttt agtagttgga atagttgttc
    14101 ttttttctag cgtttttgtt atatatagta ttttttatat ttcagttgtt aattcagttc
    14161 agatgtatgc taagttgaaa tcgttaggta tgacttcttt tcagttaaaa aaaattattt
    14221 ctctacaagg aaatattttg tcaattattt ttattccatt aggggttata gcttcttgta
    14281 taattgctta cataattcaa ccattagcat ggcaaatgat agctgattta tttataattt
    14341 taattttatc actggtaatg tttttaactg ttagaatttc tttatttaaa ccagctagaa
    14401 ttatttctaa aatatctgct attgaagcca tgcaatatac agaaactaaa tttagaaaaa
    14461 aagcaaaaaa ttttagttat ataaatataa aaaatctagc attaaagaat atcgagtcaa
    14521 atagaaagaa aaatttgatt gcattaatat cgctaagcat tagtggagtt ttatttattt
    14581 caatcgctaa tcttgctaat tcaatagatt tcaaaaaaca actatctcaa caatttgtct
    14641 ataacgaaga ttatataatt agcattaaga gtgataactt atatgagcat attacagaaa
    14701 tccaacaaaa taatccacta acagattctc ttaagaatga aattaaatct attcatggag
    14761 taaaaaaatt aattgaatcc aaatcaatta aaagtaatat tatagaacct tacatcaaag
    14821 atagagacga aaaacaattt attactctaa ttaaaggtat tacgcctgaa ctttcaaaat
    14881 cacttgaaga atatattgtc agtggaaaaa tagactatac acatttagat tcagatagtt
    14941 taattataaa taaatataga acaaaatatt atggattaaa tttaaaagtt ggagatacag
    15001 ttacatttaa tatctttagt ggaaatgata ttattaaaaa gaaaatgaaa attataggaa
    15061 tcattgacaa ttccaataca ggaggtatgt tttttgcttc agacaaaact ttagaatctc
    15121 taacaccata taatacaaat ttagattttt ctattttaat agataaaaag cattctaaag
    15181 aagttgaaga aaagttgtta agtataattc agaaaaattc aaatttaatg ctttattcat
    15241 atgaagatga ttatagaatg attactagag catttcaata tataattata gcttcataca
    15301 tttttgttgg tttaatttct tgctttggta tactaaatat gaccaatacc ctaataaaca
    15361 gcgttttaat tagaaaaaaa gagtttgcat tacttcaagc cgttggaatg acaagaaagc
    15421 aactgcgaaa tatgctttat agagaaggtc taaacataag tatcaaagct atatgtactt
    15481 caagtacact aggttatttt ggttcaaatc tactttgcac ttttataaaa gatgtaatta
    15541 gattagactt tattaatttt aaattttcta tatttactat tttaattttt tcattcgtat
    15601 taattggtat acaagtttta gtgactgaat tacttgtaag aaatatagaa aaaaaatcac
    15661 ttacagaacg cttacgttct cagtaagaat gtatataaaa aatagccatt ttttatacaa
    15721 gttttaatat gattttttaa aagctatagg gatagaaaat tttgacttta cataattttc
    15781 tatccctata cttgttttta ttttatacag atacacttac ttatatctcc gagcaacatc
    15841 accagagaca taaacaagca tattccttat tttctaaata tgtcaattgc atgacttgta
    15901 acccctacta aatcttcacg ttcacaagta gcaaggtgat attttaaata taatgcaaat
    15961 gcatcatcat ccatagagtc tactgcttca cgcatatata aagccaaacc atctgatgcg
    16021 acataatgca gtctagttac attaaatgca gacatcaaat catcaatgtt ttctttacgt
    16081 acaagctcaa acaaatcctt tggctcagac ttagcagcaa atgtctgtgg atcaatcaaa
    16141 cctttttcaa tatattcaga tacattaata tttcctctat ggaacccctc gtcaatgaga
    16201 cagccatcag atataacata tgcagcaaaa accacaccac caggttttgt tacacgaatt
    16261 gcctcatgta aagcttgttg tttatcttct ttgttataaa gatgatatag tggtcctaac
    16321 agtaacgtaa tatcatattt attatctggg aaaacagata aatccatagc attaccctga
    16381 gtaatgctta tattttcatt tgattgagta tttttatgga aaacctctat attatgttcg
    16441 actaattcca ctgcgtcaac atcatatccc tgacgtgcca gtgtgtgaga ataacgacca
    16501 gttgcagcgc caatttcaag cacacaatca cctgatttta tgtacttatc aatgtaatgc
    16561 atagtagtga gaaattccac cattccatgt tttaatgcaa gtcgactatc ttcatcataa
    16621 gtattataaa agtcaattaa gtattgtttt gtttccataa tttcacctct tctatattag
    16681 ttaataaaca tcaaatagac aaattccaat ctggtttttc ttgtcttata aaagctgtta
    16741 atatattttt caacattttt tcagcactat atctatctaa tgttaatttt tttgttgctg
    16801 ttaagacagc tatgccatga gtataaagaa ataaatctaa aaatattact tttgcacatt
    16861 ccaactctat tccaatgctt tccgaaaaaa gtcttgcctt tttttcattg tcaatttctt
    16921 tataaaaatc atttgcctct tccatcatca aatctaaatc atttataaat aacaacttaa
    16981 ataaatgagt ttcttcttga gcaaattcaa tgtatgaaag cggaagcagt aaataaggac
    17041 ttacactttt agaattacta taattaacaa catactgatt atagtattca tatgcaaaag
    17101 caagaaattc attttttaat tcattcatgt tttcgtaaca agtaaaaatt ggtcgagtgg
    17161 aacactgtaa tttacttgca atactcctcg catttacagc ttcaaatccc acttctcttg
    17221 taatatccaa gactgtattc aaaatcattt cttttgttat ctttacttta ggtggcatat
    17281 atgttcacct ctccattaaa tttttataaa acacatatgt tatataacta tagtaacata
    17341 tcaagaaatt atgtcaagaa aaagctttgc atttttttgc aattcctaaa acttataaat
    17401 aaaacatgag ttgcaattaa tctattaata gataacagca actcagattt tcctttaaat
    17461 actcttcttt ttgtctaata tttcatatac acaatcttct attgaattaa tatctttttc
    17521 ttttcttttt ttagaatcaa ttaaaatgaa atacagtata aaacctacag caagtcccca
    17581 tgttccgcct ttagcagcaa ttacacctcc cataagtcca atcaatccct tctcaacacc
    17641 tgtattacat agctccattg atagctttgt acataaatat ccttgaacta aaagtgttac
    17701 tgacaatgct aatggtataa gaggtgtagc aatctgcgca attggataaa aacagataaa
    17761 aattgctgaa acccatacta atgtacccat gccactaaaa acagactgca tggcttcctt
    17821 tccaaccttg tacctttgag caacacttcc tgtaagtgtc gcagctaatg gaccgcacat
    17881 tggtatatat ggagaaaaga ttgccataaa aatatttctt atcccactta taacattaga
    17941 cctatttgaa ttgaaatcta tatattcatc ttttctattt tcactttcag aaagtactaa
    18001 gttttcacct gttataaaat caccaaaggc aattatgtat atcataaaag ccattggtat
    18061 cccttgtata aataacatta cacttggaaa acctattcca aaaggagata ctgtatttat
    18121 cagattttta aaatctacta tgtaaactaa attgtcaaaa ctaaatctag gaatttctat
    18181 ttctttacta aatataccta ctattaaact cactaaaata gctgatacta caccatattc
    18241 tcctaaatga aataaaaatt tattcttaac ttttagagta tcaaatcttt cagaaaacat
    18301 tacaaaacaa gcaaccaaaa ttcctactgt tatagatata ggataagaat caaatctccc
    18361 tttaggatta aactctgata ttatagtcga tataccgaca cccattagta ttccaccttt
    18421 aatagaattt ggtatcttac taactaactt tccagctaca ccagttatac ccattaaaat
    18481 aaatattatt cctaaatcta tttgtaaagc tgttaatgct tgtattcttt caatgcccat
    18541 gctgaacttt aacaaatatg cagttgtaag agttatagaa gctgtaatcc agccaggtac
    18601 aactgggtct cccataaaaa catgcaaaca gtataatatt gagttaatga tagccatact
    18661 ccatgccact tcatagggta atcccaaaat ttgtgtacta aggccaattg tgcctaaact
    18721 tgtcatactt gatattatac cttgaatagc ttctggtttc tcaaatctgt aatgtataaa
    18781 tggaattctt atcttaaaca ttccaaattt tatatgaggt tgttcccttc tagcgagtct
    18841 ttccataact tcactccacc tttcaataac tcttataagt ttacaacttt ataattgttt
    18901 ataaattatc aatgtcatta aaattagtat ataacatgca aattatataa acaataaatt
    18961 aaatctataa aattgataga ttttgtgcat atatagaaag accgcttcat ttttagttac
    19021 ttaaatcact taatattagt tatttaaatc acttgataaa ggttttttat aaaattcaat
    19081 agttctattc catgattcaa actgtccttt tgaatttcct tctacagttc cacctgaaga
    19141 aaaaatccct ctactcatac tcatacataa agtctccttt agaggcataa tatatggaat
    19201 tggtaacgga taacccatat tattgtatgt taataattct acgttatatg gataattatt
    19261 ttcttcaaag atttctaatg cctctaaaca agcttcataa ctattccata tgttatcttc
    19321 ttttccagca atcataagta tatctgcttt ggaattttct atttttattc tagcctcttt
    19381 tctattctct gctcttttaa gacttttctt ataagtacta ttaaatccaa atggaatatc
    19441 ttttgatata ttttttttct gttcttcaaa gaatatatca ttgtctactt tgatatatgg
    19501 aaatggctct cctttatatg accatgatga agtatcattt ccactcatca atccattaag
    19561 agcttggaaa caataaatgt gaggttgtga tactacaacc tttttaattt gttcatatct
    19621 tgaagcaagt aaaaaggcca attctccacc ctttgatgtc ccatgtacaa atatttcttt
    19681 agtactggta atttcatttt cactaatcca ctcaaatact ttttcaaaat attctaatgg
    19741 aacctcttct aatttatctg gtaacccttc taaagcaaag tatgcaactg ttagtacatt
    19801 gaatcctcta gatgcaagtg gagctgctaa aaggtctaga gcgtctaaat ttccatcaga
    19861 accacctagc attaaaattg ttttattatt agaattttgt ttataaaata attttcctat
    19921 aaactcatca gatatgtatt ttgtggtaat atcttctgat ttaaatattc tctttaattc
    19981 aacttctttt ctttcaatag aagtttcaaa tatcatattc ataattattg gtttatcaat
    20041 agatatattt tctgcaaaat ttttaccttc agttttagat ttttccagcg aataaattaa
    20101 ccccatacaa ttatcagcct tataagtacc ttctataggt tcaactaaat ctaaatctac
    20161 ttctcctttt tcatttgaac aaaacactcc ataagatgaa aattcttctc ccttacacca
    20221 tggaaattcc attttcacac ttattttaag tttagtattt ggaattaagt ctgaaaccat
    20281 aatcttaagt ctttcatcca caaatgattc tacattgtat acatttactt tcataatcta
    20341 tttctcctta taataattat ttatgtttta tataaaacag ctttatctaa tacaagataa
    20401 ataaattttt aaattaagac accatattga ctatttcatt ttatcaacat atttcatctt
    20461 tgtcaacata ttgtcttttt tagtaattta aaattatggt ctatatgaaa tccagttgat
    20521 ttttggctaa ttaaagatag gaatgctatt aggtttagaa tatatatatt caaaataaaa
    20581 aaccactcat ttaaagaatg gtttttaata taaactattt gttattaata aggatatttt
    20641 tttatttaaa tttatataat cgaacatcta atatttaatg tttaataaaa ttatgcattt
    20701 gcaaaagtat atatggcatt aactcttgct acatataaat tctcatcact acatccaata
    20761 tatctagcat atttttcaat cttttctttt gcattttcaa catcattttc atcgctctcc
    20821 attaactcat ttatataaat atccattaat gacgctttat attctcttat atcatagcct
    20881 aaaaattcag tcaatctctc aatttcctta ctaaaatctc catcttcttt taccaaaaat
    20941 tcgtatacat cttccctata tggatttaaa gttattattt gttttactac tttgcattcg
    21001 tcagccttcc caataaaacc atctttataa ttattaaata aggcattaga tttttttata
    21061 gattcttcat caatataaga atgtaccgca gttatacgat tttctataag ggcatctaca
    21121 acagcataat gaattttaaa acaatcattg tataaataat tgctcatttt ctttattgtc
    21181 gattctattt ctaagaagct cgcgtggcca ttttttttat ttttatttgg tataataaaa
    21241 gttttattca aattattaaa aagtctctta aaatctaaat atttttcaca ataaacactt
    21301 ttaaaggtat cgatatctat agttataatt ccaaaactta ctatagtctc aattcctttc
    21361 ttaaaagact cttctatata tttttctcct aaacttaaac ctctatcaga tatttgcttt
    21421 agattttgaa aactatttaa acaatctctt tcaaagtttt tagatatttc taaagcatat
    21481 aatttaaaga ttttccttat cttattataa ttattttttg tatcatcaaa tctgataata
    21541 gctccattta catcaaaagt aatcattgag ttttaacctc cccattagtt tcatcaataa
    21601 aataaattta atataaactt tataatcata atattaaact tatttatact ataattatta
    21661 atttgatgat aaaagctatt ttccttctaa ataaaaaatt tgtctaaagt ttctaaaaaa
    21721 tttagacaaa tcttaggtta ttactcttta aattattttt aaattattcc aaatacgcca
    21781 aataatattg gtataaatat agctggtaac aaattggcta cttttatatt cgttaattta
    21841 agtacattta gaccaagtcc tataattaat aaacttccag ctgctgtcat ttctgtgact
    21901 acaggatcac ttaaaaatcc atttaaaaat gatgctccca cacatataat accttcatac
    21961 aaaaatacag atattgcaga aaacattaca cctataccta aagaagcagt aaatataatt
    22021 gaagctattc catctataac agattttaca aataaagtat catgacttcc tgtaagacca
    22081 ctttctaatg aaccaactac tgccatggct cctatacaga ataataaact agatgatata
    22141 aaaccctctg ctatagaatc ttttttattt cctttagaaa atttactttg taaaaaagca
    22201 cccaacttat ttacccattt gtcaatatct attaattctc ctatcagagc acctacagct
    22261 acggatatta ttgtcactaa tgtgtttttt ccttctagtg ctcctgatat accaatgtac
    22321 aatacacata aagcaattcc attcattata gtatcgctta ctctttgagg taatcctcct
    22381 tttattataa gacctactat acatccacct gcaattgcta gactatttgc tattactcca
    22441 aacatttttt ctcccctcga ttagctaatt gaaatttatc ctattattaa aacctaaaca
    22501 tctatacttt gtattggata ttttaaatat tccaaaaatt cttcaaaacc ttttctagat
    22561 atatttacag ttttagtatt tatgttagga tgaaaattta agtaatcttc tttaattaca
    22621 tctttatcta tgtaaagctc aacttttctt tctgtatcat ttattaatga aaaaggattt
    22681 acacttccag gaagaagttt aagtacattg tatagtcttt caggtgaagc aaacgataat
    22741 ctagaacttc ctatcttatc tttaagtgag tttaaatcaa cttgtttttc tcctttaact
    22801 acaataagat aatacttatt tcctttggca tttcttaaaa ataaattttt acattgacac
    22861 ccctttgtta ttccatctaa aatgactaat tgctctgctg tatatacagc ttcatgctct
    22921 actacatcat attcaacatt taatttatcc aaaatatcat atattttagt ttcattcatg
    22981 ctagattcat tttcatcttg ttctttttta ttatttaaat cactcatact tttctcctta
    23041 aacacttcat cataatatca ttgtgaggta ttattctact atagctattg ctcttactgg
    23101 agctccatca gcatcattat acttaaatgg tgctgctata aatagaaatt tttctggaac
    23161 attttctaaa tttgtaagat tttctatgat tatttttcct ttttcaaata aaatatgatg
    23221 cacttcaaat actgatgtgt catatctatc cactgatagc atatctattc ctatgccttt
    23281 tatattagta ttagctatat agtttgcagc ttcttttgtt aatgtaggat aacctacata
    23341 gtactgcttt ttatcccaaa attttgacca acctgattta aatattataa aatcactatt
    23401 ttttatttca tcttcatatt tttttagata ttctaattct atattctcat ttcccttttc
    23461 aagttccaat acataagctt ttccaacaaa attttctata tccaacttat ctagggtctc
    23521 acctttagta tacatatgtc ttggtgaatc catatgagtt ccattgtgag aaaatacact
    23581 aattaatgtt tcttgataac cattttcttc aataatagct actttcttga tatctggtct
    23641 attaggctct gcatatacag gcatgtcatt atgtgtcaca tgagttaaat caaaaacctt
    23701 catctttata taccccctat gtttttattt tctattatat acctaaattc attttatttc
    23761 tatcagattt tattccatta tataccatac tactatatat tttttataaa cctcaaggga
    23821 tttaccgaaa aatcttggaa agaattatca gtaaatataa catcttcacc caaaaaacgt
    23881 ttataccttc cttcttcttt ctttaaagta attctctgtt tttgtgaatg attattaaaa
    23941 accattatct taagaggagg tttttttcta ttagtctgca ctctttttaa catgtatttt
    24001 atgtgcatat cagcttcagg aaatgaatat ccacaaaata ttaaaaggtc agtatctctt
    24061 aaagtttttt ctgtttcatt ccaaacatta cttaaaaata tgtttgacat attcttaaaa
    24121 tatgttggtg gcactataat aggttctgtt aactcaccac actctaaaca ttttgtctct
    24181 gaactatttt caataagctt catgacacca ccttcatgtg gtgtaagagt tacactatta
    24241 caaatggggc aataaagcca attcaatgac ccatgtatct tatataattt gattaatggt
    24301 tcttttggtt ttctccaatt taactcctct ttaaaatttg taaactccac accataatca
    24361 agcataattg gcaaatcatt cttatctctt aatccagtaa ctgtattgtc tatatgtata
    24421 tcataatttg cactaataaa agtagtatca agaagcaaat cattttccaa aagattttca
    24481 actaaaagtt tatgatattt attatctgtt ctaggtgtat tatgaattgc ttcagccatt
    24541 agcagtatta aatattgtct taaaaaacgt atactatcac ttctgttatt tagattttct
    24601 agtccaaaat ttctaaatgc ttcttttctt tgttctgcta agtctaaaag acctaaaact
    24661 tcttcaaagg taggaaaatt tgcattatct atattatctt ttactatatc aatattgaac
    24721 atctgtttaa agaaaatata caattctcta ttcattttgg tattataatt tgtgggattt
    24781 agttttttaa aatattcgct aaaaagttca ttttgaatag gtagattctc tgctgctgaa
    24841 gctcctgctc ctaaaaatat tgctattttc atatagttcc tcctaatata aatattactc
    24901 ctatttttat atccattaaa tatccatatc atttatttta tgatacaaca ataaagctgt
    24961 cttttactag aaaaaatcct attacaagac agccttatat tatattaaat ctttaataat
    25021 tcgtaaatag aaagagcata tatttgagta gcttttataa tatcctctac tattaagtat
    25081 tcattagctt ggtgttctgt ttctggtttt cctggaaaaa tagctccaaa tgcaacacaa
    25141 ttatctaaag ctcttgcgta tgttgctcct ccagatgaaa gtggtgttcc atcaagacca
    25201 gtttcttctt catatacttt tctaagtgtc ttaacaagaa gagtatcttc tggaacatag
    25261 attgaatcca agaaatcata ttcttcataa tttaaattgt atttatctgt cattttcttt
    25321 aactctttta caaaatcatc ctttttgtat gttactggaa tccttacatc tattcctgca
    25381 aattcttttt cgttatcaat agttactcta cctatattta ctgttagttt acctgataca
    25441 tcatccttgc aattaggaat tatattgttt ccattagcat cttcaccaat aacttctgct
    25501 aaaaacttaa ttatattaga atctatacct atattattaa gagctataca aagtctagcc
    25561 actgcattta taccagtatc acttgctgct gaatggacac tttttccaat tatacagatt
    25621 ttattacctt caacagtata ctcaaaattt aatttatcaa gttctttttt taatttatca
    25681 gagtattttc caatatagat tgctttacct ggaactgcat ttaaagcctt tccaactgac
    25741 aactctatat cacttttgct atcacaagtc aaatgaactt gtaaaagacc tttctctgca
    25801 tttgttattg ggaatctaga atctggagta aatccatagt ttggtatttc ttcattattt
    25861 tctttatatt tatttataca tctccaaaga ttttcttcat cagccccaaa aatgaatctt
    25921 atcctcttat taaagtctac atttaaatcc ataagagctt tcactgcata aattgctgat
    25981 attgtaggtc ctttatcatc ttgtgttcct cttccataca atttaccatc ttctaaaaca
    26041 gcttcaaatg gagggtaatt ccagctttct aaatcacctt ctggaaccac atcaacgtga
    26101 ccaagtatac ctatcatttt ttcaccttgt cctatttctg catatccata atatccatct
    26161 ttgtatactg tcttaaatcc taaagattta cataagtcta atatacctct taaagcttta
    26221 tctacatttt caccaaatgg gcaattttca gttgcttcag atataacact aggtattttt
    26281 acactttctt gtattgaaga aatcatttca tcttgtagag aatttacttt ttctttaatt
    26341 tgctctttca tattatcacc cttcaatttt catcataata tactaataaa tattttgacc
    26401 ttttaaaaaa aaaatatgct tattttttca gtttttttat aaattcaatc gcttttaaag
    26461 tccccatttt tgaatggtca aatgtaagtc caccttgaaa ataaacatta taaggaggtc
    26521 ttattggagc atctgcactt aattctatag aagaaccttg tataaatgct cctgctgcca
    26581 ttatgacttc actttcgtat ccaggcatgt cccaaggaac aggttttaca taagaatcga
    26641 ctggtgcagc ttcttgtata ccttcacaaa aacttattac ttcatcagca ttattaagtc
    26701 taatacattg aataatatca cttcttaagt catcatattt tggtaataca tcatatccta
    26761 atttttcaaa agctcttgag caaaagatag ctcccattac agcctgagat actatataag
    26821 gcgccaagaa aaaaccctga agtacatttc tagttgttcc aaaagtaaga ccacattctt
    26881 ttcctattcc aggagaagtc attctataag atataagctc aatcaaatct tttctaccag
    26941 ctatatatcc acctgtcaat gcaagcccac caccaggatt ttttattaaa gagccagcca
    27001 taacatctgc tccaacatca gtaggctctt tggtatctaa aaactcacca taacaattat
    27061 caaccattac tatagcttct ggtttaacag actttattac ttctatagct tcttttatat
    27121 cactaattga aagtgatttt ctccaagaat atccttttga cctttgtatc ataacgagtt
    27181 tagttctatc atttattttg tttttaattc cttctaaatc taagtttcca tcctctaaaa
    27241 aatctacatc atcatatgta acaccatact ctttcaatga acctttttct tctcttatgc
    27301 ctataactcc ctctaaagta tcatatggtc taccagtaac agataaaatt tcatctcctg
    27361 gtctaactat accttgtata cataaagtaa gtgcatgtgt accatttaca attattggtc
    27421 ttacaagagc atcttctgta ttaaatactt tagaataaat ttcctctatt ttttcacgac
    27481 caatatcatt atatccatat cctgtagtcc agttaaagtg catatcactt agtttagatt
    27541 cttgcatagc ttttaaaact ttatattgat tatattctct tatttctttt atttcttcaa
    27601 acttatcttt tatttcttcc attatttctt gcgatagttt aaaagtatca tcatctattc
    27661 catagtagtc ttttaataat tcttttgtct cattaagcat tttatcacct gtctctttat
    27721 tattttattt aaacatgtaa aggagactat tttactaaaa caaataaatt atagtttata
    27781 agttagtata agtctcctct caatcatctt aaattaaatt aattatctat ctgttgttat
    27841 tattattgtt gttttggttt tgatttgtta gattaatata ctttccaggt tgtatagttg
    27901 atacagcatg tttataaatc atgttttgtt gtctattatc cccttctatt aatattatat
    27961 aactatcaaa cccctttaca agccctttaa cttgcactcc attaactaaa tatatagtaa
    28021 caggtatcct ttctttcctt gcattattta aaaacaaatc ttgtaaattt aaaactgtat
    28081 ttttcattag atttgtaccc tcccaagatt aaattgaact tctaaaatat tcctacctta
    28141 tattctatat aagttatgac aaattttcaa tattaagttg tatacttaaa aatttatata
    28201 ttattttata gaatctttaa tatataagat tatttcattt tttaattcgt ctatattttc
    28261 atattggtct aaatcaaacc attttgctgt tttatatctt ttaaaccatg ttatctgacg
    28321 ttttgcatac cttctagaat ctcttttaat tatttcaata gcttcttcgt atgtatattc
    28381 tccatccaga tactttaata tctctttata acctatacct tgcattgata tcatatcttt
    28441 tttaaatccc atacttaaaa gtttttttgc ctcttcaacc aatccatttt taatcataat
    28501 atcaactctc atatttattc tttgatacaa atgttctcta tctctattta gaactattat
    28561 tattggttga tattcttcat taaattttaa atcacttgaa aaatcattca ttttcttacc
    28621 acttagacaa acttcaagag ctcttatgac ccttttagta ttgtttttat ggattctatt
    28681 agcagattct tcatcaagtt cttttaactt attatgcatg taatctattc ccttttcagc
    28741 aagctgtttt tgaagttctt ctcttaattc attattggca tcagattgtg caaaatccat
    28801 attatatatt aaagagttta aataaagtcc tgtgcctcca gttactaaaa cacatttttc
    28861 cttttcattt atttcatgta tatatttttt tgctctttgt tgaaactctg aaactgaaaa
    28921 tggatagtca ggcttaactt catctataag gtaatgcttc actccttgca tctcttcctc
    28981 tgtaactttt gcacttccaa tatccatata ttcatatatt tgcatagaat cagctgaaat
    29041 tatctctgca ttcatatctt ttgctaattt tattgacaaa tctgtttttc caacagcagt
    29101 tggacctgta agtattataa gtggtatttt tttcataaca ttcctttcca tatattttaa
    29161 atatccaaat attttacata attcttttaa acattttttc tatttctgtc ttagatattt
    29221 cgaccattat aggtcttcca tgaggacatg taaatggatt ctcacacttt tctagctgtt
    29281 ccaataaact ttttatttca atatcgtata ttttatcatt tgcttttata gcagacctac
    29341 atgccataga tgcaaatctc tctcctttta aatcataatt acttgtaatt tcttctatat
    29401 tatcaattat ttgaagtata aatttctcag tttcaggtac tccaaatata gtaggaacac
    29461 atcttaccat tatatgatta ttgccaaaaa tttctagctc aaaaccaaat ttcataaata
    29521 actccaagtt attttcaatt tgaagcatat ctatatttga tacttcaata accactgggt
    29581 ctaaaagtat ttgcatattt atatcttgtc tatagaactt ctccatatat ctttcatata
    29641 gtattctttc atgtgcagca tgttggtcca acaaatacat agaatcgtct ttgctcaaaa
    29701 ttatataagt attaaataca actccaatta caaaatatcc gtataaagaa aacttgcgtt
    29761 ttgaactaca caagtattct tcttcactgt ccttaatact atctcctaag taataatttc
    29821 cttcattttt aattccatca acttgaaact cttcttgtat attatcattt gcactatctt
    29881 cacttagata attaatgttt tttacatcat cttctacact tgcatttagg acttcactta
    29941 ctgattgaaa ttcatttatt ggtttttcac ttgataaagt aacaacttca atgacttcat
    30001 ccttaccttt agtaatatta ctatttttag gagtagattc taataaatca ttatttctta
    30061 atttataatc tgtacttttt tctctactgt taatagcgat tctaggttgt gtttttttat
    30121 cactataagt agcatatttt ccaataagat ttgagtgtat taatttaacc tttaaaaaat
    30181 ctctaagttc tatatacact tcttgttctt tttcaaattt aatttcaagt ttattggggt
    30241 gtatatttac atctatacat gatgggtcta cttctatatt taagaagcac actgcatgtt
    30301 tacctattgg aattatagac ttataacttt ctgttattgc atctatgatt attttacttt
    30361 ttacaaatct tttatttata tagatatgtt gtaagttttt atttgaacgg tatatattgt
    30421 tattacctat atatccattc attttaaagt gattgcactt aaactctaca tctattatat
    30481 tttccgttat ttcttttcca tatatacttc taatagtgtt tacaagtttt ccatcccctg
    30541 gagtatttag catctgttta ttgttatttg tatatttaaa ttgaatgttt ggattaccta
    30601 ttgcaagttt atttatcaaa tcacttatgt ttatagtttc tgcatgagta gattttaaaa
    30661 atttctgtct tgctggtgta ttaaagaaaa tatcttttat aatgatggta gttccattag
    30721 tagaacctat tggttctttc gatattattt ttccaccttc aacatatatt ttagttccta
    30781 ttatttcatc tttagtttta gtcgtcattt ctaattttga tacagctgaa atagatgcca
    30841 atgcttctcc tctaaaacca agagagtata aatcatacaa atcatcaatt tttttaattt
    30901 tactagtagc atgtctcaaa aatgattttt caacttcact tgaaggtatt cctattccat
    30961 tatctgtaat cttaattaga ctttttcctc catcaattat atctatggaa attttatttg
    31021 cacctgcatc aatggagttt tcaataagtt cttttactac agaagaaggt ctttctacaa
    31081 cttcacctgc tgctatctta tttatagtca aatcatctag gatgttaatt atatttttca
    31141 tttttacacc tctaagattt tatttctttt gcttttcttt gaagattata aagagagttt
    31201 atagcatcta atggagtcat atttaaaata tccagattta aaatttcttc tgatagtata
    31261 tctctattta cactatcaaa agagatttga gttaaggcta cttcttttac agaatcttct
    31321 tgattaattt tctctttatc atcattttgc ttcattaaag caacttcatc atttaatgct
    31381 ttaaatttag aatttaattt atcataatcc ttttttatat gtttgtagtc ttttactata
    31441 agttcatgtt ctatttttag agattcatat ttattcttaa aattactttg atttacctta
    31501 actaattctt cgtccaaatt actattaatt atattattgt tttctttatc tccatttatt
    31561 gcaacactgt tgtaaacatg atttttttcc aagtctttaa gtatatattt tgctctttct
    31621 atgacttcgt ctggtaattt agctaattta gcaacatata ttccataact cttgtctgct
    31681 ccctgaggaa ttatttttct taaaaatatt attccttccc catcttcttt gactgctatt
    31741 gaataatttt taacctcttt aaactcttct tctaaatcag taagttcatg ataatgtgtt
    31801 gcaaataatg tcttacatct aatatttttt tgaatatatt ccactattga ccatgctaaa
    31861 cttataccat cataggtact agttccacga cctatttcat ctaatataac taaacttctc
    31921 tctgttgcat tttttaatat aagggatact tcgttcatct ctaccataaa agtagattga
    31981 ccttgagata aatcgtcact agctccaact cttgtaaata ttctatctaa tattggaata
    32041 tcagcagact ctgctggtac aaatgagcct atatgtgcca taagagctat tatagcagtt
    32101 tgcctcatat atgttgattt acctgacata ttaggaccag ttattatgtt aataatgttt
    32161 tctccacgat ttaaataagt atcatttgga acgaaatttt cctctcctac tatattttcg
    32221 acaacaggat gtctaccatt tcttatatca agtttattat tttcatttat tgctggtttt
    32281 acgtagttat ttatatgtgc aactgttgca agggacacaa aaacatctat attagctata
    32341 gtcttagcca ctttttgtat tctatctatg tttttatata ttgtatctct tatctctaca
    32401 aatatctcgt attctaaaga tttaatcttt tcctctgcat gtagtatctt ttcttctatt
    32461 tcctttaatt ctggagttat atatctttct gcattactaa gtgtttgctt tcttatatat
    32521 gtctcatcta gctttgcctg tttaaagttg gcttttgtta tttctatata atacccaaaa
    32581 actttattaa atcctatttt taatgattta actcctgttt tttctctttc cctgttttct
    32641 atttccttta caagaaaggc tccattctta gaaatctctc ttagttcctt aagttcatca
    32701 ctaaaatctg atttaattat gtttccatct tttatggtta tcgtaggttc ttctagaata
    32761 gcttcatcta ttaaattgta tatatcatct aatttatcca tttctgaaac atactctttt
    32821 aggatttttg catttgataa atttatagta tcttttaaat taggtaactt ttctatcgaa
    32881 ttttttaagt gaattaactc ttttggtgtt actctctcaa atgctatctt tccacatatt
    32941 ctttctatat cgtatatatt tttaagtata tcatttaggt cttctcttaa tataaagtca
    33001 tcttttattt cttctattac atctaatcta ttttcaattt tagatttatt tatcaaaggt
    33061 tcttctacat attttcttaa aagtctacct cccattgctg tagaagtttt gtcaagtaca
    33121 tgtaataatg aacctttttt cttattccct cttatagttt gagtaagttc taaattagtt
    33181 ctagtaaaca tatctagaac catatattct gatgaattgt atatatttat attattaatg
    33241 ttagatgtta tttgtttttg agtattgtat atataattca gaagaattga taagcttgac
    33301 tttattagac ctttatcatc aaactttagt ttttgtagat aaacatctgg aaaatattct
    33361 tttaatatat ttatatccaa atagttatca ctaaaacttt catttatgta tatattactt
    33421 acagtagcta tatctctaag cttttctata aagtctaaat catttataat tatttcagtt
    33481 ggatggattt ttgcaatttc ttcaattacc ttatcttcat ttaaacatgt tgcattagtt
    33541 tctcctgtac ttatatcaac ataagttaga cctatattag taccatcctt atacaaagat
    33601 aataaataat tattcttttt attttcaagt aaatttccat ctaaaactgt accaggagtg
    33661 atgaccctta taacctctct tctaacaatt ccttttgctg tagatgggtc ttccatttgt
    33721 tcacctattg ctactttata tccattttca actaattttg atatatatga attggctgag
    33781 tggaaaggaa caccacacat aggagcacgt tcttctaaac cacaagcttt tcctgtcaat
    33841 gctatttcaa gagcttttga agctactaat gcatcttcaa aaaacatttc ataaaaatct
    33901 ccaagtctaa aaaacagtat acagtcttta tatctatttt ttacttcaag atactgtttc
    33961 atcataggag ttaatttatt catatctatt tgcattatca aacctccttc ttttctaatt
    34021 aaatattata tcatagtaga tgattattat taacacaata tataaaattt tgacatataa
    34081 ctaaaaacaa tccaaaatgt atactcacat tttggattgc taagtttaat gcttattaaa
    34141 taaaaatatt tttttttaca ataaagatat tatttctttt ttaagtttaa tgatattttc
    34201 aaggttatct tcatttaact ttctactatc tataacaaat tctttcttta ctattgatgg
    34261 tttttttgaa ataacataaa ttctgtttga tagcattata gcttcgtcaa tatcatgagt
    34321 tataagaagt attgttgaat ttactttttt cttaacatct aaaagccatt tttgcatgga
    34381 tgttttagtt aatgaatcaa gtgccccaaa aggctcatct aaaagcatta tgtcattaga
    34441 atttataaaa gttctcaaga agttagctct ctgtctcatt cctccagaaa gttctgaagg
    34501 atatttatct tcatatccgc ttaatccaaa cacctcaaaa taaggtttta ccattgacct
    34561 agctttactc tttttttctt tttttaaaat tagtggtagt gacacattat ctattatagt
    34621 tttatatggc aagagtaaat ctttttgata catataacta tacttaccat ttatattcac
    34681 tttgccacta tcataatctg taagtttagt tataatatta aaaatagtac tttttccaca
    34741 tccacttggc cctaatatag aaacaaactc accttcataa acatcaatac ttatatcttt
    34801 taaaacttca atatctttat aagtttttga tacattttct atagatactt ttactttttt
    34861 attgtggtaa aaattcattt gtgtaagcat cacttgcctt catatcctta tctattaatt
    34921 tatattcttt taagaatgat gtatagttgt tccatacact atctttcatt tctccccatc
    34981 gtggtgcatc atctttgtac ttgcttgcta aatattcttg actctttaaa gctagttttt
    35041 catctacttc aggtgcatgt tttactaaaa tttttgctga ttcttctgga ttttttattg
    35101 catattcata accttttgtt gtagctttta agaatttttt agcaagctct ggattatttt
    35161 taagcacagt ttcattagaa attataagtg gtgtataata atccaatctc tcatctaaat
    35221 cttttactgg tataaagttt aagtcatatc caattaaatc agctttaact gcatcccaac
    35281 cttcaaatat ccatgcaaaa tctacagttt tcatagctgc aaagaaatca tcttgaccag
    35341 tattaactat ctttaactta ttaaaatcag ctccattttt cttcataact gctttaaaaa
    35401 cagcttcttc tgatggagag ccccaaccac cataagtttt tccttcaaaa tctttagctg
    35461 ttgttatatt cttttctttt ggtgatgcaa acccagaagt attatgttgt ataacagttg
    35521 ctacagcttt tattggaagt ggatcttcac tagtctttgc ataagtaact tgttcttgat
    35581 agcttatacc aaagtcagct tttcctgttg ctactaaagt ttctgctcca ctttcaggtg
    35641 gttgtactat ttcaacatcc aaaccttctt ctttatagta acccttatca agtgctacaa
    35701 ataatccagt atgatttgta tttggtgtcc aatcaagcac catagttact tttttagaag
    35761 aagtatcttt atcattgttt ttgtctttat tgttatttga agaacatcca gttattgttg
    35821 ccaatgtaaa aacacttact aaacctaata ttcctaactt tttaaaattc ataattgcct
    35881 ccctcaaata cctttaatct attaatctat gtcttataac aacttttttt acagcctgta
    35941 caattcctac aaacattaaa ctaaatataa ctactaatat agtagatgca aatactttat
    36001 caagtgcata tgcacttttt gacctaagca tataaacacc aagtcctttc gttccaccta
    36061 gccattctgc aacagttgct gccattacag catacgttgc agatatttta agtccagaaa
    36121 aaaatttatc cattgccata ggaaatttaa ggtgaatgaa tgtattaact ttatttgagt
    36181 ccatagtctt aaataaattt aaataatctt tatcaatatt ctcaatgcca tctacaaaac
    36241 ttattaatat aggaaaaaaa caagtaagca ttaccatcaa tacttttggc attgttccaa
    36301 atccaaacca tatcataaag agtggtgcta taattattgt aggtatcatc tgtgtaacca
    36361 acataatagg ataaagacat ttttttatta ttgatacaaa atccatcaat attccaatta
    36421 ccaatgatat tataattgcg actataaaac caagtattgc ttctcctata gtaaccacag
    36481 catgtatgga taaattttga taatctttaa aaaacacatt aattatatct actggtgtgg
    36541 gtagtatata ctgaggaact tctaaaactc ctaccataga ttgccaaaga ataagtataa
    36601 acaaaaatgt tgctattgga tatatactat ctttgacttt atctatgatt tctaagtttt
    36661 tcatctatag tcagacctcc agctttttca tctactctag ctactgttat gactctttca
    36721 catccaagat taaaagggat tttatgtacc ttttttaata attcaaaaac ttcatcaaaa
    36781 tcaccttcaa cagtagtgct cattgctcct atttcacatt taagaccact tttttcaatt
    36841 tcagcaatac aagcatctac tactttatac atctcttctt cacctttgta aggtcttagt
    36901 ggcattactg ctatgtctgc gattaccatt taaatcactc ctttaaaaat aattaatacc
    36961 tactaggtat attttgtttt ttattaaaaa attttattac taaaaagata ttatatttaa
    37021 ataaaagaaa agtcttatcc aaaataggat aagacttatt tttatcttaa ctggtctccc
    37081 tacactggca ttatccaaac aggttcaaag ggtcgagata aaatctcctc tcagcacatt
    37141 agctccccga aatatattat atcttttcta cttgattata ctacacttat catattttaa
    37201 caaatttttt atttatatta gatattttat ccgttcaaaa atatctaata taaattgtac
    37261 attattacac caaataaatt atcttaaaat ggtaatttat caacatcttc caaaactaga
    37321 ttaactgaat tttctagttt ttattttgct atttcaagca tatttggatg tatatcaatt
    37381 ccatacaaat attgtgctaa atttctgttt tcactcatct aatttgcaat cgttacaaat
    37441 tacagattag aaatagcttt tagactctac ttatctttat ctacaaaaaa tccttcaata
    37501 aattcattaa taatattcag taatctaaaa ataaacctct atctagattt ttaagataca
    37561 agccttttag gaagtatctt ttgtgataat ataaaaatta ctcatcatta tcatcaaaat
    37621 cctcagcaac caacatttta gataagaatc aaactctata atataattat tttatccaat
    37681 ttatttttga gaaataaaat atttttttaa tttttaaatt ttttaatatg caccttgaaa
    37741 aaagaaacgt tttcctatat tatggtaata gtagaataaa actaaatagg caaaactaga
    37801 gaaatttagt gacgcaaagc tatagggact aagacttatg aaatgagtta tgtcagccag
    37861 ttgccaaaga gatattttct ttttgttttt atgaatgttt tttaggggga tatatgatat
    37921 atgtttatgg ataaaatgga tagatgtaca catatactaa ctgcatatat atgcagttca
    37981 cgcgattact gcaattttat agatacacaa ttaaatgatt ttatactaga atatggagag
    38041 aatgtggtag aatcttgttt gcatcaagtg atggtattgg taagcagata taattaaaga
    38101 aattttattt tctagaaaac cctttttagt tttatctacg ttttataatt cattaaagaa
    38161 ctaattaaaa taagcttcat tatatatttt tcataaagta aacagttgag tttatctcat
    38221 atactgttta ccccaattac ataattctaa taatatagat tctaatgatt taccacgttt
    38281 agtagacaaa tattcaactt ttggaggaat agtcggataa gaaatcctct taataagtcc
    38341 atctctttca agatacttta attcttgtga cagtatctta tgacttatat gaacaacttc
    38401 tttttttaat tgactgtaac gtattggctc ttgttttaaa atatgccata aaataattgc
    38461 cctccattta cttcctatca cagtcatggt aaaaccaatt gaacaattaa aatctttttg
    38521 tctttcttca atatttggaa attcttcaaa attattatct atatccatct caatcctctc
    38581 tttcaaaata gtaagtatct aactaaaaag tgcattcttg tttagcgtac taattcgtaa
    38641 tataatcttg attgtagtat agagccttac ataatactac ttttttagat gtatatgttt
    38701 aaactttaat atagacatat gttattttat taattttatt taaacagata ataataaata
    38761 ataatttatc tcaagttttc tatgtagata aactaatcta atgtttagaa aggattatat
    38821 aatatgaata aaaaatttcg tctttatcaa gttgactcat tacaaaagaa aaatttacag
    38881 gtaatccagc tggtgtaatt acaaatgctg atggcttatc tgaatcacaa atgcaaaaaa
    38941 tagcaagaga attaaataat tcagaaactg cttttatttt caataacttg gctaaaaatc
    39001 atcaagaaaa ttatgatgta catatcagat tttttacact aactaatgag gttcctatat
    39061 gtggtcatgc tactattgct gcccattatg ttagagcagt agaaaaccaa ttcaataaca
    39121 aaacagtatt tcaaaaaact agtgctggaa tattaccagt tgatattata aaaaaagata
    39181 atgattatga aataattatg acacaaggaa aaattgaagt atctccaccg ttacaacaaa
    39241 cctatcaaaa aagattactt aatgcatttg gtattttaaa ggaagatatt cgtacagatt
    39301 gtcctattgc catatcatct acaggacatt caaaagttat gattggaata aaggaattat
    39361 caacattaaa tagtttatct ccaaatttat atgaattatc gaaaataagt caagaaataa
    39421 aatgtaatgg atattatatt tttacattac ataataaaga aactctacaa gttcatggaa
    39481 gaatgtttgc tcctgcaatt ggtatttcag aagaccctgt tacaggaaat gctaatggac
    39541 ccttaggtgc gtaccttgta cattataaag taatacctac acccaacaca gagtttatat
    39601 ttgaagcagt tcaaggagaa acaataaaac gcaaaggcaa gatgttagta aaagtaatta
    39661 ttttagataa taaaccagtt gaagtaaaaa taattggaaa agctgtaatt gcattctcaa
    39721 caaacataga aatctaatta tagattcctt ctaagtaaat ttttgaactt tttaatacta
    39781 atttaataac aaactaattt atatacaatc aaattaattt ttttattatt tgtt
//
LOCUS       DAMKMI010000019        39711 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMKMI010000019 DAMKMI010000000
VERSION     DAMKMI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 39711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.019"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(76..480)
                     /locus_tag="PC429_000956"
     CDS             complement(76..480)
                     /locus_tag="PC429_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF3846473.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPLIYLKYKPDK"
     gene            complement(672..1859)
                     /locus_tag="PC429_000957"
     CDS             complement(672..1859)
                     /locus_tag="PC429_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF3846474.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1891..2316)
                     /locus_tag="PC429_000958"
     CDS             complement(1891..2316)
                     /locus_tag="PC429_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3846475.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            2774..3523
                     /locus_tag="PC429_000959"
     CDS             2774..3523
                     /locus_tag="PC429_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF3846476.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(3689..4054)
                     /locus_tag="PC429_000960"
     CDS             complement(3689..4054)
                     /locus_tag="PC429_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3846477.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(4070..4846)
                     /locus_tag="PC429_000961"
     CDS             complement(4070..4846)
                     /locus_tag="PC429_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3846478.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(5293..6720)
                     /gene="ascB"
                     /locus_tag="PC429_000962"
     CDS             complement(5293..6720)
                     /gene="ascB"
                     /locus_tag="PC429_000962"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF3846479.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(6781..8709)
                     /locus_tag="PC429_000963"
     CDS             complement(6781..8709)
                     /locus_tag="PC429_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3846480.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(8854..9696)
                     /locus_tag="PC429_000964"
     CDS             complement(8854..9696)
                     /locus_tag="PC429_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3846481.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(10043..11413)
                     /locus_tag="PC429_000965"
     CDS             complement(10043..11413)
                     /locus_tag="PC429_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3846482.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            complement(11452..12123)
                     /gene="pgmB"
                     /locus_tag="PC429_000966"
     CDS             complement(11452..12123)
                     /gene="pgmB"
                     /locus_tag="PC429_000966"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF3846483.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            complement(12125..14515)
                     /locus_tag="PC429_000967"
     CDS             complement(12125..14515)
                     /locus_tag="PC429_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF3846484.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVEDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(14541..15989)
                     /locus_tag="PC429_000968"
     CDS             complement(14541..15989)
                     /locus_tag="PC429_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF3846485.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMIIGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(16392..17279)
                     /locus_tag="PC429_000969"
     CDS             complement(16392..17279)
                     /locus_tag="PC429_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3846486.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(17379..17828)
                     /locus_tag="PC429_000970"
     CDS             complement(17379..17828)
                     /locus_tag="PC429_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846487.1"
                     /translation="MEIREILTEESIILDLKSTTKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            complement(17818..19725)
                     /locus_tag="PC429_000971"
     CDS             complement(17818..19725)
                     /locus_tag="PC429_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3846488.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(19993..21888)
                     /locus_tag="PC429_000972"
     CDS             complement(19993..21888)
                     /locus_tag="PC429_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846489.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(21924..22802)
                     /locus_tag="PC429_000973"
     CDS             complement(21924..22802)
                     /locus_tag="PC429_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF3846490.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(23507..24940)
                     /locus_tag="PC429_000974"
     CDS             complement(23507..24940)
                     /locus_tag="PC429_000974"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF3846491.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(24963..26804)
                     /locus_tag="PC429_000975"
     CDS             complement(24963..26804)
                     /locus_tag="PC429_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3846492.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(26930..27763)
                     /locus_tag="PC429_000976"
     CDS             complement(26930..27763)
                     /locus_tag="PC429_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3846493.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(28807..29019)
                     /locus_tag="PC429_000977"
     CDS             complement(28807..29019)
                     /locus_tag="PC429_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3846494.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(29020..29436)
                     /locus_tag="PC429_000978"
     CDS             complement(29020..29436)
                     /locus_tag="PC429_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846495.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(29506..29919)
                     /locus_tag="PC429_000979"
     CDS             complement(29506..29919)
                     /locus_tag="PC429_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846496.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            complement(30753..31382)
                     /locus_tag="PC429_000980"
     CDS             complement(30753..31382)
                     /locus_tag="PC429_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF3846497.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(31461..32423)
                     /locus_tag="PC429_000981"
     CDS             complement(31461..32423)
                     /locus_tag="PC429_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF3846498.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(33185..36883)
                     /gene="cbpA"
                     /locus_tag="PC429_000982"
     CDS             complement(33185..36883)
                     /gene="cbpA"
                     /locus_tag="PC429_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HDF3846499.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNAGGDANAGNAGGDANAGNAGGDANAGNAGGD
                     ANAGNAEEDSNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVVEEATAED
                     PTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAEL
                     IFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRAGKGTTLT
                     IDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSLKVVGNYY
                     SAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDITINGGTIT
                     AKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGGAHGSAFG
                     DGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKATTFKSLG
                     GAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAPSYTDDQG
                     VLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYEEFTIDDT
                     PPYLIIKRYDGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTISSQRLNEE
                     TMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAEIIPADTE
                     TSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGKEYKDPVE
                     LKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGSEDEKKLK
                     LDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGN
                     GDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGGYDTGNPN
                     LNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGN
                     GGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNGGNSKPDI
                     DTDGDGKPNINIDIDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPDI
                     NIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQNVQTGDQS
                     NIMLDLALMFISLFFLIKNLTNKYLRRK"
     gene            37152..37724
                     /locus_tag="PC429_000983"
     CDS             37152..37724
                     /locus_tag="PC429_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF3846500.1"
                     /translation="MEKLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
     gene            complement(38724..>39711)
                     /locus_tag="PC429_000984"
     CDS             complement(38724..>39711)
                     /locus_tag="PC429_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF3846501.1"
                     /translation="KLETRGKMPKWYLELINKVKNILENMLYNRNLSDEQIFYLNLFL
                     DIQNLFLGIYELKSESVIFENTLDDNKIIAKMDTYIIIDYINASFEYLGYVLNGSALN
                     SIDYGVPQNRERFVLIGAKKDFLKNKKIETPKPIVGENYVTVRDAIGDLAEYEASKGS
                     MDYCFEKNSNNIEKDFYRKIILDSNKIYNHVCTDTRNIALKRFEYINQGNNFHSLPDE
                     LKGTYADPERTQNTIYKRLVYDKPSDTVVNVRKSMWIHPIKNRAVSAREAARLQSFPD
                     SYKFLGTKDSVYQQIGNAVPPLLGRVVAEKILNLFNDEPNEYLRDVFEALDG"
BASE COUNT        12866 a         6996 c         4130 g        15719 t
ORIGIN      
        1 aatttagttt tatttaaacg ctctagtgat atttttaaat aaattttaat aaaaatcatt
       61 ctattttgtc ataaatcatt tatctggctt atatttaaga tatattagtg gatactttac
      121 actttttaat tcctcattac ttcttaaaag tttattatta ttttccatat cataaatttg
      181 taacattact ttatttacat atagagcata ataattatca ctataaaaat attgatactc
      241 atatcgaggg tcatctttgt aatacacttt gtatagtatt ccttcgtaac ttgccttctt
      301 ggctttttct tttgtaatac tttttatatt atcttttgaa atatcttgtt ttaccatata
      361 cttatcaata tatgtttcag catctttcca tggtttaatg cagtttttat atacaacaaa
      421 ccctgttaca cttataatac ctataattaa tactataaaa ataactttat attttttcat
      481 attaatcctc cttcatttat tatttaaaac tcgatataat gcactaaaaa aaggatgtta
      541 tcttttttat taaaatataa catccctcat atttagcatc aatatatatg ccccaactgg
      601 taggttaaaa gaccccactg tcagtacttt gattaaatta catgtatgta tatatatttt
      661 taacaaatct attatatgtc aacttgtcta ctattacata taatctgtaa tattacatct
      721 gctgcttttt ttgagcctcc tgcattataa aagctttttg atagtttagt tgcattttct
      781 ttatattcat tgtcatttat aacttgaaat actgcttttt taatactttc tgacttatcc
      841 tcttttaaca taattcctgc tcccaaatga acaactctct tagctaccat tctctgctcc
      901 gaatgttgag gaaataatac cataggtact ccataatata aactttcatt aacactattc
      961 atcccagaat gagtaacaaa cacatcagtt ttttgaagta tagcaatctg ttcaacacta
     1021 tttttgacct caaaattatt tggtatattt cctaaacttt taatatcagt acttctacca
     1081 acagacataa tcacatcaac atcagaatct ttaaatgcac ttatacagtt tttataaaag
     1141 ctaacatttt tattattaat tgtaccaagc gaaatatata ttagctttct ttttctctct
     1201 ttaggttcaa taatcaactt tgatactgat ggacctacaa atgaatactt actagagaag
     1261 gtttctacca ttggttgaaa ttcttttgat gtatatacaa tggtatccgt atcattatca
     1321 ttagaaacta tagaaataaa attttttact tcataccctt ttctttgtaa aagctttatt
     1381 tttttattaa tgcgtcttac accaaaaatc atacgtataa tttctataaa gttttgtttc
     1441 atcaactttg ctgtatgttt attaaatgca aaagttgttg tagagcatac atatggaata
     1501 ttaagttttt gtgcaaataa ctttccccaa aaacttaatg aatctgatac aatgcaatct
     1561 ggattaaaat ctttaagttc tctacaaact ttttcatcta aagaaatagt agtatctact
     1621 atcatctcaa ttaatccagg aaaatcctta ccaatttttt tctcatctcc agggagtaat
     1681 tctggaaggt atttatcaca acaaataaat tttgctccag caccttcaat tttatcttta
     1741 aattcattaa aagaataata caatacctca tttcctctat ccactaattc acgaactact
     1801 tcaatagtag gattagtatg accatatgca ggaatgctaa aaaatactat tttactcata
     1861 tttcatacct ccttttataa tttttttaaa tcattcttct ttacgtacta ctcccacatg
     1921 acctataaaa tttatcaaat cacgttgatg aacaattcct aatcctcttt cctcatctcc
     1981 aagtaccaat aatttatctc ctgcatttat attaaatact tctcttgctt cttttggaat
     2041 gacaatctga ccacgttcac caactacaac tgctccaaag aaaaactttc ctttaggagg
     2101 tactataata cctgtttttt cttctgaatg attaactaag tcatctagtt tgacattgta
     2161 caattttgca agtttaatgc aactacatat atcaggaata gattctccac tttcccattt
     2221 tgctattgat tgtctagaaa cattaagttt ttcagctaat tcttcttgtg tatactggtt
     2281 gatttttctt agacttttta aattctcatt aatcatattt ttcatacctc ctatgtttat
     2341 aatattggat ttattattta aattctatca actaatctta acattcatat atatatttat
     2401 atacttttat tatataaatt tattattcag gtaacatatt tccttaagtt atcgtaaaca
     2461 aaaaaacaag cacaaatcta ctaactgttg ttaaagtatt taagttaagg tcatttacat
     2521 tatcaaatat ttctacacct ttaaaataac ctttatattt ataatctttc ataagcaaat
     2581 tattatccct tctcatatac aagtccagtt gatgtcttgt tgctaaattt aatggtaatc
     2641 aaattcttta ccaataagta taaaaattac taatgtgtta taattttctt aactactaaa
     2701 ttttacatac atttgaatag aatatatcaa taaattacta taataaataa aaaatagtta
     2761 gggagatatt attatgattt tatattttag cggaactggt aatagccgat atgtggctcg
     2821 taaaattgca caagaattaa atgatgaact gatatcacta aatcaactta taaaagagga
     2881 aaaaacagat gaattaatat cagcagataa accattcata tttgtatgtc ctacttatgc
     2941 atggagattg ccaatagtag ttacggattt tataaaagaa acaaaatttc taggaagcaa
     3001 cagagtttac tttgtaatga cttgtggtgg agatacagca aaatccataa actatattca
     3061 aaaactatgt aaatacaagg aatggcaatt aaaaggtatg gcagaaatta aaatgccaga
     3121 gaattatata gctttatttt ctacaacaga caaagaaaca tcaaaacaaa tgattaaaga
     3181 agcagataaa caaatatata ggattatttc tgatatacgc aatgaaaatg aatttgaaac
     3241 tattactcct agtggcttag gaggtacaat taaaagtggt ataataaata cagtttttta
     3301 taagacaatt attagtgcca aaggatttca ttatactgat aaatgcattg gctgtggaaa
     3361 atgtgtagag ctatgtcctt taaataatat taatttaaaa aacaaaaaac cagtttggaa
     3421 aaataactgt actcattgta tggcatgtat ttgtggttgc cccactgaag ctattgagta
     3481 taaaaataaa actcaaaaca gagaaagata ctatttagaa taatatattc ttaatattta
     3541 aaccattata aaatgaacta tacccattgt tttatacagt tttacatatt ttaaccgtct
     3601 cctaaaatgg gtatagttca aaagatagtg tctcaaagtg aattttttag ttcctaaatc
     3661 aatctttttg tattaaatca agtataattt aatcaccttt tcttatatca ttttcactaa
     3721 tatgttgtat tccaacaaat ataagattta ttagctctgt tagtgacttc cattgtgatt
     3781 tttttgaatc aagataataa taatttttcg ttccttctct acgcaaatta acaatctctg
     3841 cttcttttaa tatcttaaga tgatgagata cagcaggacg agaaagatgt gttttttttg
     3901 tgatttctcc cactcgtata ccattaaaat cactctctaa aagagcaatt aaaatcaatt
     3961 gtctagtctc atcacccata gcagagatag ctttgcgaca ttctttaaat ttagacgtta
     4021 tttcttgcat tctttgttga cattcttttt ccataaaatc caccctctct tataagtatc
     4081 taacagttgc tacttctttc tgtgcagtac gtctatattt aacatctggg tatccaatta
     4141 ctaatgttgt aagaacatga ttactacgtt ttagacctaa aagctttttt aattttggcg
     4201 aattatttgc tacatctgaa aaatatccac taaataaaac acctagacca taagactctg
     4261 ccattaatgc catatttgat gctgcaagtg aaccactaat cttatctttt gtaattatca
     4321 ttattgcaat aggagcatgc ttaaagaaaa agttatcgtc aatctcaact tcttttgaat
     4381 atttaatagc tatatttgca ataggtttta tttttctaaa aaatttcact gcttcgtttt
     4441 cataaattga ctttttattg tctaaaacaa tatatgatac atcttgtgaa tttttagcac
     4501 taggggtata cctacctgcc tcaataattt gctttataat ttctgaagaa acttctttat
     4561 ctttaaattt acgtatactt cttctagatt taattgccat tagtaattca tctgaatcta
     4621 gctttggctt atttgtaagt tctataggag gttcatcaaa tcctgtcaag gtaatagctt
     4681 ttgtgggaca tattgctgca caatgtccac acattagaca atcttgtttt ttaattactg
     4741 actttttatt ttctataatt atattgttta ctggacaatc atttttacaa agcccacatc
     4801 caatgcaaag ttctttgtca acttctatta tatgttggta tttcatattc attcatctac
     4861 ctttcgttta ttagtttaaa ctattataac acgtagacat tttttatatt cattggtaaa
     4921 gaatttgatt accattaaat ttttaatttc tctattaaat aaatttttca tagaatttta
     4981 aaatatcata gtttatacat aataaaaaag acaagcttaa aacctaagct tgcctttttc
     5041 atcttttatt acaaagtaaa ttgcacttac ttcccatact tcttaacaat tttattttct
     5101 aaaattttat ttgtgaatca tactattgca tcaatacaat gtaaaataat tctatattga
     5161 ttttctatag ttaaaaaagt taaaaattat acttattatt aatttttata ctcttcttat
     5221 ttaatacaaa gttcttattt aacatagatt tcttgtctaa tatagatttc ttatttaaca
     5281 tagatttctt gtctaatata aatcttcacc atttgaagca attactttct tataccattc
     5341 aaaacttttc tttttcattc ttcttagact tccattacca ctattgtctc tatctacata
     5401 tataaatcca taacgctttt ccatttcacc tgtaccagca cttactaaat caataggtgc
     5461 ccatgtagta taacctaata aatcgacccc atctatagaa actgcatcat gcatagcttt
     5521 tatatgttct cttagataat caattctgta atcatcttct acataaccat tttcatcagc
     5581 tatatcttta gcacctaatc cattttctac aataaatagt ggcttttcat atctatcata
     5641 tacatgattt agtgtaattc tcaaacctaa tgggtcaatt ggccatcccc attgactgta
     5701 tgaaagatat ggatttttag cagatgtaaa tatgtttcct tcagccatat catctgcatt
     5761 ttcatcggtt ctaacacaac ggctattgta atatgaaaat gaaataaaat ctactgtata
     5821 tttattcaat aattcaatat caccaggttc catttctggt gtaatacctt tttgctccat
     5881 atactttaac gcatagttag gatatttacc tttagattgt acatccacaa agaagtaatt
     5941 ttctctatct aactttgtag cttcccatac atcattagga ttacaactat aaggatatac
     6001 acttccagct gcaaacatac atccaattaa gttatttgaa tcaacttcat gtgctatttt
     6061 agttgcaatt gcactagcta caagctcatg atgggcagct tgatatttta tcttttcaac
     6121 atcttcacct tcttcaaagc aaataccagc acccataaat ggagcatgta aaatcatatt
     6181 tatttcattg aaagttagcc agtacttcac taatccttta tatcttctaa agataacatt
     6241 gcataatttc tcatagaaac ctatcatttt tcgattacgc catgcaccat actttttaac
     6301 taaatgcatt ggacaatcaa agtgtgtaat agtgactaaa ggctctattt tatatttttt
     6361 acactcctta aatatatttt cataaaattg aagtcccttt tcatttggtg tttcttcatc
     6421 accttttgga aatattcgac tccatgcaat gctcattcta tatactttaa atcccatttc
     6481 agcaaataac tttatatctt ctttataatt atgatacata tcaataccag ttttagctgg
     6541 ataataatgc tcatcatcaa aatcaaacat ttttagtttc cctgtaataa ctgctgttct
     6601 atcttttcct gttggacata catctacatt tgccaatcct ctttcgtctt cattgtatcc
     6661 accttcacat tgattggccg cagtagctcc tccccataaa aaatcattct taaaactcat
     6721 agtcaaagcc tcctaaaatt ataaccaaag cactagatta tgctaatacc tcaatcttta
     6781 ctaaaataag actgtaatta gattgtcttc taattcaata tttttacttt ctgtttctac
     6841 tacatctaaa aattgtgatg aatttgtaat aacaattgga gttacagtac tataccccaa
     6901 ctcttttatt gcataaacat caaagtttaa tatagtttga ccttttgtaa ttctatcacc
     6961 ttgttttata catgcctcaa aacctcttcc ctctaattga atagtattta atccaatatg
     7021 aataagcact tcaacacctg tatcagaaag aatccctata gcatgtagac taggaaataa
     7081 ggttgtaact accccatcta ctggagcaac tacttttcca tcactaggta ttattgccac
     7141 cccttgacct agtacaccag atgcaaatgc tgcatcctct atatcactta attttaatac
     7201 ttcacctttt ataggacttg ttactatttc tctttctaat ttagtagact tgacttcttt
     7261 tatttcttct ttacccttat ttcttacttt tttagactct tctactttct catctttata
     7321 taaaatcata gttaatacaa aagctacgac cattgatata gctataccag aaagagctac
     7381 tattaagtta cccattcccc catctggttc aatcattgct ggaagttcaa atattcccat
     7441 tcctccagtt ataaattttc taagattaaa gaatccatag aaagcgcctc caataccact
     7501 agcaatacaa cttattataa atggtttctt taaaggcaat aatattccat aaattgctgg
     7561 ctctgtaact ccaaatatac cagatataaa gtttggtatt gccatctctt ttagtttttt
     7621 atctttagtc ttgaaaaata ttgccaacac aactgccgaa cttgcaaatg tacaggcaaa
     7681 gaatggcatc ataacattat catatcctaa agtcataaca ttgtttatat atacaggaat
     7741 aaatccccag tgcataccaa aaataacaag aatttgccaa gtaaatccta ctatagcacc
     7801 tgccaatagt ggactaaaat ctcttattga aattacaatt tgtgaaataa gtgtagaacc
     7861 aaatgttgct atagggccta taactataag tgcaacaggc actgttatta ataatgtaag
     7921 cattggcaca aagaaaaact taactaaatt tggaacacac ttattaaata atttttcaca
     7981 cctagaagca aaatacacta taaatataat tggtacgact gttccagtat aatcaattga
     8041 tacaattggt agtccaaaga aatcaatata tactggtgat gcaaacattg ttcctttaaa
     8101 taaagtgtac atcacatctg ctcctgctga tatactgcta ccttgtatag caggataaca
     8161 tactgcagct cctagtgcta atcccaacat tggttttaat ccaaacttct tagctgaagt
     8221 atatcctaag aacaaaggaa ggaatgtaaa taatgcatct cctgctgcat ttattaccat
     8281 ataaccacca gatacatcac tatataatcc cattgctaca aataatgcat taaatccttt
     8341 caacatacca caagctgcca ttattcctaa cactggctga aaaattcctg atattatatc
     8401 tataccttta tctagtatat tacctgattt tttagtttct gaacttgtac ttgaatcatc
     8461 taaaccaatt atttcaacta catctttata gacagcttca acatgatttc ctataactac
     8521 ttgatattgt cctccacttt tcattacagt aacaatacct tccatgtttt ttaatacatt
     8581 gtcatttgct atactttcat cttttaaagt aaaacgtaat cttgtaatac agtgtactaa
     8641 cccactaata ttttcttttc caccaacatt gtttactatc tcttgtgcta attgtttgta
     8701 ttttcccatt tttctcctcc atatttcgtt taaattaatt ttttgaaatt tgaaggtttg
     8761 gccaactcaa tgttaccatc caaccataag tataattttg ttagtatcac tcctttcaat
     8821 cattcttaat atactactga aaaattattt cacttacata tttgagtctt taactacttt
     8881 agctaaatgt attgttaaat atataagctc atcatctgtt aatatgtagt tatattgttt
     8941 tttaatgaaa tttttaattt taactacaca ttcaaactct ttattatatt tagatttaat
     9001 aatgcttagt aattctttgt ctgtctcacc ttcatgtgca ctatctaaaa gcaatctttg
     9061 cgcaaaaaac tttaaatgca ttataaatct ataataaaac actgattctt catgaaattc
     9121 tatcctaaaa tgatacctaa ctatgctaag tacttcttgt attaacttcg taatatcaga
     9181 tgccaaagta tgaccttcac ttaactgagc attcactata tgaaaggcta taaatccagc
     9241 ctcatcttct ggtaatttta tacctgtttt gttttcaata atatctagtg ctttcattcc
     9301 aatttcaaac tcttctttat aaaatctttt aatttcccaa aggattgcat tttttacatt
     9361 tattccttct ttcatccttt gaattgcaga atatgtatgg tcacttaagg atatgtaaat
     9421 actttcattt agcttcttgt ttaactttat ttgtgcgtat tctataattt cacttgataa
     9481 tttcatgtgt tctataggaa tattattgag taattcttgt agtttgtttg atatttcttt
     9541 atttgatatt ctaaatactt tatttacttt agttttatct attagattgc cttttgattt
     9601 ttgataacca attcctttac ccataacgat aacttcttca tcattatcat ctaatgtgat
     9661 tacaacatta ttgtttaata tcttattaat tatcatgatt atcctccttt taagcataaa
     9721 aaaagcttat ccataataat gggcatactc cttcctagga tactgctatt attaatgaat
     9781 aagcttagct tgtacttaaa caattactac ctaattccaa ttaatatgat aaaacatcag
     9841 acatattttg tcaacttttt atttccatat acttcatcaa tcttttatgt tgctttttgt
     9901 cagttctaat aattattctt attaatatta acttatttct ttgcatcaat ataaatacaa
     9961 aacaagttat agtcaataat aataaaaatt ctattaatgt aaatagttaa tattatactt
    10021 cctctactac attatctgta ctttatttaa catttttttc aactccaaaa acatatgtca
    10081 taataaatgg agcaaccaat gctataaata taactataag tccatttact atatttgaaa
    10141 tccccttttc ccctgataat ccaagtactg ccattatact attttgtgga acatataaag
    10201 tcacatgagt aattcctgca aataaccctg cgattcctgc tcctatagat gctaacatag
    10261 caggtttttt aagtttcaat gtaactccat aaagagctgg ttcagttata cccatgatag
    10321 ctgatatcca tgcagctgat gctaactgtt tcatcttact atctttgctt cttaatgcta
    10381 cagctaatgt tgcgccacct tgcgctaaat tagcacaaac ttgagatatt aatattagcg
    10441 attcttgtcc tgtagttgca aatgctgata tgaatatagg agtcaatgca tgatgcatcc
    10501 ctgtcataac gataaatggc attgcagctg ctaataaaca aaccattata aatcctaacc
    10561 ttccttgtag taaatttatt atactagcta atccctctcc taagaaattt cctattggtc
    10621 caattactat taatgctatt ggcgcactta ttaataatac taaagttgga tttaaaattg
    10681 tttttaacat gctaggtgtt aatttatcaa tgaatttttc tatatatgac atcatccata
    10741 ctgttaaaag tgcagggata acagatgctg catatataac atcttgaatt ggtactccta
    10801 taaatgatat agggtcccca cttgataccc atgcagagaa atttggatgt aaaaacacac
    10861 ctactactat aactgctatt gatgtattaa cattaaattt tttagctgca tttattgcaa
    10921 gtattatagg cataaagtaa aatgctacat ctcctattat atctaatact ctatatgtac
    10981 ttgaatttga tgatagccaa ttaaaagtgg tgcaaagaga taatataact tttatcatcc
    11041 cagatgctgt aagggctggt atcataggtg tcatacaacc agttattgta tctattatat
    11101 tatttaaaat actatttttt ttatttttat tttttatatc gcttttaata tctcctgtgg
    11161 tatttatccc cttatttatt attgcattaa atacaacacc tacctcatta cctattatga
    11221 cctgatattg agttgaagtt gagtttactc caactacctt atctatagat tttaatttat
    11281 ctgcattgac tttcttttca tcttttaaga tgaaccttaa tcgtgtcata caatgagtta
    11341 agtcaattat attatccttt cctccaagtc catcaattat caattgagct acttcttcat
    11401 atttattctt cataccttat cctctctttt aaaataattt ttaatataat tttaatccaa
    11461 attaatcttt ttactccaag cttgtaatag tgtattgtaa tttaataagc tagtatcttc
    11521 tacaatatgg tcacattttc ctagtacttc ttttttcccc acaccaattg atgtcatatt
    11581 agcagcattt atagctttaa tacctgaata agaatcttct attcctacac aatcatattg
    11641 ttttaaattt agaatcttgc tcccttcaat aaaaatatca ggatatggtt ttcccctttt
    11701 taataaagat ggatttacta tacaatcaaa ttcatctttt attcccaaag attctaatat
    11761 cctaggtgca ttcttactag cagatgctat tcccaatttc acattatttt ttttaagttc
    11821 ttttataagc tcatttatcc ctggtaatat atctttatat gttaatgagt ttattaactt
    11881 tatataaatg ttattttttt cttcacacac ctttaacttt tcctctttag tataactatc
    11941 tactttatta gcagataaca atattttatc taatgattct tccctgctta tgccttttaa
    12001 tttttcatta aaagtttcat ctatttctat atctagcgat tttgctatct catgccaagc
    12061 ttcaaaatga tacttagcag tatctgttat aactccatca agatcaaata agactgcttt
    12121 catattaatt actcccttta aaacaattat taacttctat tatgttcttt gataatttta
    12181 tttctttatt atcatgtaat atggttatat ttctaccttt gattaactta taataagatt
    12241 tatattcttc tatatttaca gaaactaaat tatctctaaa taatatatta aatttaatat
    12301 ttttccagtg tttaggtatc cttggttcaa aacttaattt atcatttttt attttcattc
    12361 ctgcaaatcc atatactaaa ctcatccatg ttccacccat attagcagca tgtattccat
    12421 cacttgtatt tttttgtagg ttattaatat ccattaatgc tgtgtccata aaatagttat
    12481 aagctttttc atgatttcca atttcacttg ccatcattcc aaatattgac cttgacaaag
    12541 atgaatcatg agtagttatt ttttcatagt attcaaaatc tctcttcttt tgttctaagc
    12601 taaactcatc agggaaaagc aacgctgaaa gtacagtatc tgcttgttta tttacttgat
    12661 atctatatat tgttaatgga tgataatgaa gaagtaatgg atatttttct tttggtgtat
    12721 ttttaaaatc ccatactgat ttttctaaaa atgaatcgtc ttgtttgctt attttcttct
    12781 cttcatcata tggtaaatac atattttgag cggcatttat ccagtttttc acttcatatt
    12841 cttgtaattt aacttttcta aatacttcat ttgaaaataa attatgctta aatttatcta
    12901 ccatctgtac tgcatatatt aaattatact tagccattaa attagtgtag taattattgt
    12961 ttactattgc tgtatattca tctggtcctg ttactccatt tatacagaat ttattatttt
    13021 ttgattctat ataatctcca aattcaaccc aaaatcttgc tgtttctata agaatttcta
    13081 agccattttg catcataaat tcttcatcat tagttgcttc aaaatacgtt ctaataccat
    13141 aagctatatc tgcatttata tgaaactgtg ctgttcctgc tggataatat gcactgcatt
    13201 cattaccatt tatagttctc catgaatata agcaaccttt atttattcca agttctctag
    13261 ctctttctct tgatgcatct aagattgagt atctataatc taataaagct ctggcaatgg
    13321 atggttgagt atatataaaa aatggtaata tatacatttc tgtatcccag aaataatgcc
    13381 cctcatagcc atcacctgta agaccttttg ctgatatatt agtctttcca tctcttccta
    13441 ctgattgata aatatgaaat aaattggttt ttatccccag ttgaagagtt ttatcgcctt
    13501 caatctctac atcagaggtt gtccaaaact cattcatttt ctttctctga atatttttga
    13561 ctttatcaaa tccaatatta catgcttctc ttaaaaccaa ttctaatgat tttaaatgtg
    13621 cttctgattc taaacatttt aacgactcca gacattctaa tgattttaaa cgttcttcat
    13681 ttagatttaa atcagaatat actctactat agcctacaac aatttctaaa tttaaccttt
    13741 catctttttt ggcatagact tctatttctc gctcaataca ttcatctgaa acattatagg
    13801 tctcatttgt aatatttcca caagtaatat atgaatcaaa cccacaccat aaccctaatc
    13861 tacttctttc tgtaactaaa ttcataaaat acttattgtt atattttttt acattaattt
    13921 taaattgctt cttatttata gatgccactc ttgggtcctc tactttttca caattaaact
    13981 tctccatact attttccatt ttatttttaa attttatttt tccattaaaa ttcaatggtg
    14041 ttattgtata tgaaatcatc attaattctt ctatatcttg tgatactaat ctttctatag
    14101 atactttaac ttttctacca cgagagtttt cccatataaa tgaacgttta agaatccctt
    14161 catttaaatc tagtatccta gaatgttcac ttgtaatacc ttttgataaa tcaaagcaat
    14221 ctccttcaat agagaaattt actatatgag cattaggtaa cttacagata gtctgatttt
    14281 gttttgcata gccataagcg ctttctccat atgtaattgg agattcttca taaaatccat
    14341 ttacatatgt acccatatta tctacatact cattattaaa atcagactct tcaatattcc
    14401 ctctaactcc taaatgtcca ttagctagtg taaacagcgt ttcattggat tttattgtat
    14461 ccttactaaa cccttgctct ataagttggt ccttagtagt aaatattaaa ctcataataa
    14521 cactcctttc ttaaaatgaa ttatgcatga gtttccatgt cattaaataa aatttctcca
    14581 tctgtttcta ttactttttt ataccaaaaa gcactttgtt ttttagttct tttaaatgag
    14641 cctcttccat agtcatcaga atcaacatga attatgccat atcgttttgt catctttcct
    14701 tcacttacac tggttacatc tatacatccc cacataagat atcctataag ctctattcca
    14761 tcttcattta ctccaataat catattttta atatgttctt tcatgtaatc tatacgataa
    14821 tcatcaatta tttttccatc ttcatttaca atatcttgag tacccagtcc attttctact
    14881 ataaataaag gtaactgata cctttcatat aactgattca tagttattct taaccctaca
    14941 gggtcgtttt gccatacact atttgatttt ggaagatatg gattttttat ggtttctggt
    15001 ccgtttgcct ttgatttttc taaaccttca gctctagctg aactacaagc actagaataa
    15061 tagctaaaag atacaaaatc tacagtatta tccttaagtg ttgataaatc ttcctctttt
    15121 attattaatt ttatttgatt tgattttaaa taataattca taaagcttgg atacacaccc
    15181 ctaacttgaa catcagaaaa aaacaaatta tgcctttcat gctctaaagt tgcaaataca
    15241 tcttctggtt tacatgtgta tggatatgtt tttccagcag ctaacataca cccaaactta
    15301 aattctttat ttatatacct tcctatttct atagtttttg cattagctaa taattgatgg
    15361 tgtgctgctt gatatttttt ctgaagttga ttttcatcag ctataaaagt taacccagca
    15421 cctatatatg gaatatgcat tatcatattc atttcgttaa aaggaatcca gtatttgaca
    15481 tcatctttat atcttttcat caaaatttca gcatatttta catataaatt aaccactctt
    15541 ctatcagccc acccagaata tttttcaact agagaataag gtatatcaaa gtggcttatc
    15601 gttattaatg gctctatatt atattttttt aattctttta ttatattatc ataaagttgt
    15661 aatccctttt catttggttc actttcttct cctgttggga atattcttga ccaagatata
    15721 gaaaatctaa aacatttaaa acctatttct gctaacattg ctatatcttc tttgtaattt
    15781 tcacaaaatt taatccctat atgacttgga tagtattgat tattttcatt taaaatatta
    15841 tcctcatcta aacatacatc ttttaatctc ttatcattta atgggagttt atcaaactga
    15901 gataaacctt ttcctccatc taaataaaat ccttcacttt gattggctga aattgcccct
    15961 ccccataaaa aatctttccc taatttcatg catctacctc tttcacaata taaagtttta
    16021 taatttctat ctcatattta gttataagat ttttttcata ctataccttt taaataattc
    16081 aatttaaaaa tccaattata ttttttactc actataatta atattgtcta ttttcaatac
    16141 tttcatatta aatatcaata ttagatatct atgtatataa ttatatatat agcaaataca
    16201 tttttatatc ctcattattg ttatcttatt ctcatatatt gctgtatatt tatatataaa
    16261 aggatttatt tcgaaataga ggttgtattg agaactaaaa tttagacttt ataaatgaat
    16321 ggaattttca ttgttgaaat actgaaaata tatttgattt catataaaag aaagtgcctc
    16381 atgaactaag ttcattttga gacactccct tttttttcca agctattata tcttttttta
    16441 aattctaatg gagagactcc aattttttct ttaaatactc tgtaaaaagt ttttgtactt
    16501 ccaaaaccaa cttcttcaac caaatctaca atggatatat tgttattaag taatatccac
    16561 tttgcttttt ctaatctata ttcatttaaa aaagttataa atgtttttcc tgtatttttt
    16621 ttgaaaaatt tagtaaaata atatacacta aatcctactt cataagacac atcttctaat
    16681 tttataggtt gtttataatt tttttctaca tatttgaata tattgttcaa tctctctaat
    16741 atctctttag agtttatttc atattctact gtattttctt caatttctct aggaatctgt
    16801 ctatataata taggaattaa attatacatt ttacctttta ttgcataact atatccttct
    16861 attttgtata ggtcttcatt gtagatatct atcaatatat cgaaaactga ctttcttatt
    16921 tttgctgacc aatctctact acattttttt atactactga ataaattaat taagctttct
    16981 ttttcctcat ttaataaaat taaatcatta aaaaaagata tatcaaattg aaatactaat
    17041 ctttcacttc ccggtgatgg cagtatataa tgaatatctc ctccttttat tatcactatc
    17101 tcgccttttt ttaattgcat aggcttgtca tttataaata gatttactac accttctgtt
    17161 ataagtaata tttctatttc cttatgccag tgtggtgttg ttaatacttc tccttcattt
    17221 gatagactac gaaaaggaaa ttcatgtatt aatttaggca tttctaaata tattgccata
    17281 tatatcctct cctctctagt gtctaaaaat taatttatac tatttaaaag aatacacatt
    17341 ttattttaaa aacctcaaat ttataattta tacaatacct acacttcata aaatatatct
    17401 aatatttctt tttttgtatt tgcatttaaa agtctttcac ctatattttt accacctaaa
    17461 atgtaagcta ttttagatat taatatcata tcttcattgc ctccagattg tggcatagca
    17521 aacataatta ttacctttac aggtaagcca tctatacttg gccaatctac acctttttta
    17581 gatttaccta ttactatgga tgccttcttt acatattttg tttttccatg tggcatggcc
    17641 atttgataac ctatgctagt agagcctatt ttttctctat gttttaatgc ctttataaac
    17701 ttacttttta aagttatact tcctgtatcg tataatttat ctactaattc ctgtattatg
    17761 ccttcttttg tagttgattt caaatctaaa ataatgcttt cttctgttaa tatttctcta
    17821 atttccattt gttttctcca atagattatt cttaaataat attttaatat cctctttgct
    17881 cttgcaatct attatatagt ttatatagtt cttttgccta tataaatttg atatttccat
    17941 taaaatattc tttaaaaaac tttgattact aatttctaaa taaatatcaa tcgttctagt
    18001 aacattattg atattcatta tccctttatt taatttactt tgcttatgta tatatatatt
    18061 taaatctttt cctaatgaaa tagattcata tttactccct aaattcacac ctattaaact
    18121 ttccatctga gctacatcaa aattaaaata tatattatct tctaaataca ttcctaataa
    18181 gtttgtatct aagttcatac tttttttagg tttagaaagt aaagcatttt ttatattatt
    18241 aatatcattt tccattaata gtactgatac aactatatgt aatttatctt tcaaatctaa
    18301 gtttacagta gatattacaa agtctacatc atctaaattt cgcttactca actgatgcat
    18361 aggaactata tcaactatag tccattcagg aaattctctt tttaatctgg ctgcaaggag
    18421 ctgagatgtt ccatatcctg tatgacaaac tactattact cttttactac tcatatttct
    18481 ttctattgcc gcttgaaaat atgtggcaag atacgaaact tcatctatac taatattctt
    18541 cagattatca tactctgtca taatatttat agtcatcata cataatccca gcacatctga
    18601 aaatcgctct tgtatctctc ctaacaacgg gttttttatt tggatatcat atcttaatct
    18661 gtttaacata ggccttacat gagatgctag acttttctgt agtaagtcat catttttgag
    18721 atttatattt aaaaatcgtg acatattctc tatcattata gatgaaactt tttcactttc
    18781 acttaatcta tctgattcat cattgtaact actattattt tcgctactaa atccagatga
    18841 taccaaaaaa atatatatgt acataatttc atctttcgtt atttttactc tgtatctatt
    18901 ttctatttta tctataagtg tactgacatt tttatatact aactcgtcca aattatctac
    18961 agaaatttct ctttcttcct gagattctat ttgattacct tcttcgattc tctttaaaca
    19021 tataagtata tgggtaatta aatttatata gtagggctga tttatagtat atcctaaatt
    19081 gtattctaat tctgtaatta tagtttctac aaatataata gaatcaatat caaataaatt
    19141 tatcaatgca ctaaatgtag cagaatctaa tcttgttaac tcagatatat catgttgttc
    19201 ataattttca tctaataaat catccatcat tttggcaatc gatttcctta tatccacttc
    19261 tttacctata atcttagttc cttctaaagt tttattaagt tttaaattgt attcttttat
    19321 ccatgcctct atatatttaa aatcatttac tatagaagtt ttacttacat aatatttatc
    19381 tgctaactga tttattgaag ttatattatt tgaatttagt aataaatact ttaaaatctc
    19441 cattcttctc tgctctactg ataaagattt attttccttc gattgtatat ttaaactatt
    19501 aattaagtct atattcttct ttgctaaatc ttctattaat attccatagc ctctttttat
    19561 atcaattttt atattaaaag tttctaaata tttctcaatc atcttcaaat ctttttgtaa
    19621 agttttatca gatacattta atttatcttt aaagtatttt ataggtctat atttattttc
    19681 gtttagtatc aatttaagaa attcaatttg cctcgacaca agcatactta gccctcccta
    19741 cctatgttta tatatttcta tgtttatatt aagtactatt ttaacatatt tactaactat
    19801 gtaaaattac taagatactt ctgtatcata aaagaattta tgatacacct tactaaattg
    19861 atataataga taatataatt tcaaaataaa aattaaggat atttagaagt agttaactga
    19921 ttctaaatat ccttattatt attctatata cttgttgtta ttccattaat tatttttact
    19981 attatttata atttaagcac caagtaattc aacttcacta ttttcactcc ttatagtagg
    20041 ctttgttacc cctataatca atgctgttat tactgtacca atcaatatac aagctatata
    20101 tgcaactggt gcactacatg caaatggtat tacaaatatt cccccatgtg gtgccattaa
    20161 agttatatct aaactcatac atattgcccc acctatacaa gaaccaatta tatttgctgg
    20221 aatcattctt aatgggtctg cagctgcaaa cggtattgcg ccttctgtta tgaaagatgc
    20281 ccccattata gcagttggta gagatgactt tctttcttct ttagtaaact tatttttgaa
    20341 tattaacata gctaatgata ttgctaatgg aggtaccatt cctcctacca ttaatgctgc
    20401 tgatggttca aatatattac ttgaaaacat agccaatcca aaagctgaag ctgttttatt
    20461 aataggtcct cccatatctg atgccatcat ccctgctaat attgcaccta ataatacttt
    20521 attagttcca gacatacttt gtaaccaaga ttgtaaagct tcatttaacc atgatgctgg
    20581 cgtattaact acaaagaaca ttatagctcc actcgtaaat gcaatcaaaa gtggacatat
    20641 caatacagca tttactcctt ttaatacatc tggtaaattt tctgttatct tcattactcc
    20701 taaagctata tatcctgttg caaaaccagc taccatacca cctataaaac ctgaacctcc
    20761 aataactgcc ataaaaccag ctatcatagc aggagcaaat ccttgtcttc ctgctattga
    20821 tttagctata aaaccagcaa gtatcggtat aaataactta aatgctgaat cgcctccaag
    20881 ttgtccaaag aatccagcta tagggttgta tgtttcatga gttgggtctg atgcaaatat
    20941 accaaacata aaagctatgg ctataccaat tccacctatc actacaaatg gaatcatttc
    21001 agatacacca ctcattaagt gcttataaac accctttttc tctgaccctt gaagcatttc
    21061 ttttgaatct tctaaagtct ttccacctgg tttaatttct ttagccattc cattttcagc
    21121 gtttataatt aaatttttag ctaatgaaac attcttagca tctactactt tcttttgtag
    21181 cactttcttt cctataaatc tactcagttg aacttcttta tcacaagcta ttattacgta
    21241 ttcagctctt tttatatctt ctttagtcaa cttattttct acacctatag ctccatgagt
    21301 ttctatttta acttcatatc caagttcttg acctgcttcc tctaatgctt tttttgccat
    21361 aaaagtatga gctatacctg ttggacatcc tgttatccca actataagac ctttattttg
    21421 attattacta aaatctcctg attctgcagc tttattatta aatatactta atatttcgta
    21481 tggagtttct gcacttagta gatgcttaag aaaattttct tccattagat atgtagctag
    21541 tttagatata atcttaagat gttcatctga acctgattct ggaactgcta tcataaaaaa
    21601 tgacctaagt ttttgaccat cattatctgt aatctctcta tctgttctta aatacatcat
    21661 tacaggttga tttactacat ttgacttagc atgaggtata gctattaaat tacccatatt
    21721 agttgatgat tctttttcgc gtttattaat atcagctata aacttttctt tattatttat
    21781 ataatttcca tggtacaaat gatttgccat ataattaatt acatcttttt ttgtttgtaa
    21841 ttgtttatct agtacaatac aatcgcttcg tagtatttta cctatcataa attctcccct
    21901 ccttttaatt tgttacctca taattaattt ttatttttat aaaagcactt taacttatct
    21961 atagcattga atagattcat tttaaactct ataacttctc ttgcagattt tatacatggt
    22021 ggatatatag tatctggctc atataagctt gggttttgtt ctaatagttc tctgcacttc
    22081 ttataatatg ccgacttaac atcactcgaa atatttattt tacaaactcc tagtttaact
    22141 gcttgtgcta tttcttcatc tggatttgat gaaccaccat gaagaactaa aggtatatct
    22201 actacttctc ttatttcttt taataaatct agtttaagct ctggtttaaa accttttgga
    22261 tatataccat gtgctgtacc aattgcaata gctagtgttt ctactcctgt tttttcaaca
    22321 aaatccttag ctaattttga atctgtatat attatttcat cagtaccacc ttcacttgat
    22381 gttcctgttg ttcctattgt tccaagttct ccttctacgg acacatttaa tccttttgct
    22441 atttctacta tttccctaga tatagctaca ttctcttcat atggtaaatg tgagccatca
    22501 atcattactg atgtaaatcc acatcttatt gcccttaata tttgttcttt attttcccca
    22561 tgatctaagt gtattaccat aggtacttta ctcttatttg cttcttctat gcaactagct
    22621 acaaagctat ctcctagaaa acttagctca tttgggtgta ttgcaagtat tactggtgca
    22681 tttttttcgt tagcactctg tactactgct ttaagtattt gcccacttcc tatattaaat
    22741 gctggtacag caaattgatt ttcttgtgcc acttttagca tttccttcat atttattaac
    22801 atattgtacc tcccgatatt ttaaatttta ggttgaattt gcttcttgtt aattttgttg
    22861 ttgtttatat ttaaataata tcagggtaat gaagatcaga aaataacaat cgttttcctt
    22921 agtgaaaagg aacttttatt atacactcta agttcctttt cactaaataa tttttagaag
    22981 tctgcatcat agttaattat atttctcata tacatcttct ctttttaagt gttttatgct
    23041 ttagccttct taactctttc caataaacaa ttagatagtt tagctataaa tttaaagata
    23101 ttcgtatcgt agttagcact atattggaat caaaaaaatt aaaaagatac tagatagcag
    23161 ataccaatta ttaatacacc ataagtcata gatacttcta tcttaaattc ttgcatatta
    23221 gttacaatag aaattaaata ggactattat aaaaatcgta aggtaagact gacatttatt
    23281 ctccaactat acaaatgaat actaatatag ctccttctaa ttaaatcata ctatataaat
    23341 catatatatc ccaattttta accttacctc tttatattta ctttttaata aaaaaagata
    23401 ttgactaaat agtcaatatc ttttagtgga tttaaatacc cacgatttta atctaatttt
    23461 taatggtttc cttaacataa tcttataaat attaaaattt atctacctat aaaatttctc
    23521 cattaaattt tattacattt ttataccaat caaaagattt ctttttatat cttttaagag
    23581 tcccacttcc atcattattt ttatctacat atataaatcc atatcttttt ttcatctctc
    23641 ctgttgtaaa tgatactgga tctatacatc cccaaacagt ataccctata acatcaacac
    23701 catcaatttc aatggcattt ttcatttccc ttatatgact agccaaatac tctattctat
    23761 aatcatcatc tacagtatta tctgatttta atacatcttc tgctccaaat ccattttcaa
    23821 caatgaatat aggaagttca tacttatcat atagttgatt aaggtaaaac cttagaccta
    23881 cagggtctat attccatccc caatcagaaa tttttaagta tggattttca actacatcag
    23941 cctttcctgc cgagtcatta cctgttgttt tcatagtttt atcagctgtt actacagtag
    24001 ttaaataata actacatcca ataaaatcaa ctattccttc ttttaggatt tttttatctt
    24061 cctctgtaat atctaaatta taattttttc tttcccataa ttttagtgta tgtcttggat
    24121 aatgacctct tacatgtaca tcagtaaaca taagttgttc tctattagat tctacaaatt
    24181 taatcatatc ctctggattg caagaataag gataacttgg catagctgcc accatacatc
    24241 caattttaaa ctcagggttt atttcatgac ctatcttcac aacctttgca cttgctacaa
    24301 attcataatg aactgcttga tacataactt cttctttgtc ttcctgctct tcatagatta
    24361 ttcccgagtt agtaaatgca taaataggat ttgtagttat actttgatta tttatttcat
    24421 tgaaagtcat ccagtactta actttatcct tgtatctatt cattaccact tcacagaagt
    24481 ttacaaagaa atctattaat tttctatttc tccatccacc atagtttttt actaaatgat
    24541 aaggcatttc aaaatgactt aaagttatta ctggctctat attgtatttt aataattcat
    24601 caaataaatc atcataaaat ttcaatccat cttcatttgg agttgattca tcgccctttg
    24661 gaaaaattct agtccaagca atacttgtcc taaaacactt atatcccatt tcagcaaata
    24721 atctaatatc ttctttataa ttttcataaa aattaatggc ttcatgatta ggataattta
    24781 aaccctcaat tactccatct gttatctttc ttgaaatagt atgagtacct gcagtcatca
    24841 catctgcaat acttattcct tttccacctt tattatatcc accttcaact tgatgggcag
    24901 caactgcccc tccccataaa aattcttgat tagatttcat agaaatatct cctccagtat
    24961 aattaataaa caacttttat tacaggcgtt ccaattttaa tatgtttatt ttctttaact
    25021 attacatctg ctaaatcatt tgtgttagtt agtattattg gtgtaacaat actataccct
    25081 tcaccttcta ttttatcctt atcgaaagaa acaatcctat ctcctttttt aactttatct
    25141 cctgcattca tatgagtagt ataatacttt ccatctaaca tagtagtgtt aagacctacg
    25201 tgtattaata tttctacacc tttatcagag gtaattccta ttgcatgctt tgtaggaaaa
    25261 atagttgtta ttgttccatt tacaggagac acaacttctc cctttgaagg tattattgcc
    25321 atgccattac ctatagttcc tgatgcaaac acttcatctt ctacttcaga taactctatt
    25381 tgtaagcctt ctattggaga taatatctcc tctataccat catcttcatc aaaagcaact
    25441 tcttcattac cttcttcaca ttcttcttta ctcaatccaa agaaatatga caatactgct
    25501 cctagcacta atgctgttcc tattcctaat atatatccta ggaatggtgt ataaactggc
    25561 attgagaata tattatgaaa tacatatgca ttacaagtaa caccaaatac accacatata
    25621 ccaccaccaa tacctcctgc aatagccaat actggaatca ttcttctgta tcttaatata
    25681 attccataaa ttataggttc tgttactcct gctaaaattc ctgtaactgc tgttgaacct
    25741 gctaatacct ttaattctga atttttttta gattttctaa ccttcataaa taaacctatt
    25801 gcaataccta tttgagcaaa tactgctgca acagctgcac cttctattgg gtctccaccc
    25861 attgtagcca agttttgcaa agtaattggt gtgaagcccc agtgtagtcc taatactgct
    25921 aagaatggca tagctatacc tattgcagct cctgcaatta ctctgttaaa ttcaaataat
    25981 cccataaaaa tattagatat tatatcacca acattagttg ctattggtcc aaataaaatc
    26041 acagttaaag gaaccatgac taataatgat atcatcggta ctaagaataa ttgtaaatcc
    26101 tttaatataa tcttctttaa aagcttatca acatagtaat aaataaatat agcaacaaat
    26161 atagggaata caccaaaaga ataatcaatt gctgttactg gcatcccaaa aaagttagca
    26221 ccaccttttg ctccaattaa agctgtaaaa ttaggatcta acaaagctgc tcctatcact
    26281 ccaccaacat atggatttgc actaagtttt atagctaagg taatacctaa gaatattggt
    26341 aaaaagtaaa atacagcatt tccagctgct gataatatag cataagtgct acttgtatca
    26401 gacatccatt tcatagtagt tagtattgta agcagtgcct taatcattcc tgctccagct
    26461 agtgcaggta ttaatggaga aaatgcacct gaaattgatt caaatatttt agaaactatt
    26521 gaaacttttt ctccctgttc ttcagtattt tgtccttgta aaactgcttt ttttataata
    26581 gtttcaaagt aatcagttac tttatttcca attaccactt ggtattgtcc tccactattt
    26641 actacagata gtacatcctt catttttaat aactcttctt tgttagtttt tgaagtatct
    26701 ttcagcttaa aacgtagcct agttgcacaa tgaactaaac taataatatt ttcttttcct
    26761 ccgacatatt ttataatttc ttcagactct ctttcaaatt tcatacaatc tatcctccat
    26821 tttcataatt ttatgtataa aaatagacct aaatcaatac aattagatat taaaaactaa
    26881 ttataccaac ttaggtcatg cctgccaaac agtaacaatc ctacactatt tactttatat
    26941 ttgataatct atttaaatgc aatgttaagt acaacaattc attttcgcta attttccatt
    27001 tatacgtctt ttcaagaaag ttttttactt tttttgcaca attgtaatca cttttatatt
    27061 tcatttttat tatatctaac aatgatgtgt ctacatttgt attctcacca cttaattgcc
    27121 tttttataaa acatcttaaa tgacttataa accttgtgta gtcaaaggaa tattcattta
    27181 tttcgattcc atagtgatac tttatgatat ctaatacatt atcaattatc ttagttaaca
    27241 ataaagtttc ttgcatatct tttgtttcta actgagcatt tgcaaagtgt atcgtaataa
    27301 atgctatttc agaatcagga atatttttat ctaatttttt tcttaaatat tctacagctt
    27361 tcttactaaa attatattca ttaggatata tttgtcttat ttcccattga agcgggcttt
    27421 gaaatactaa tccttcatcc attcttttta acatataact tatatgttct gataatgtga
    27481 ttaatatact atcattaaga ttatcaccca attcttctct tcccatttga ataatttcac
    27541 ttacaagctc aacatctttt aaaggtatcc tatctagtag tgaatgtaat tcacttaatg
    27601 attcctcatt attgaatata aatttctttt caattttatc tatttcaact aaatctttag
    27661 taaatttatt aaatccaatt ccttttccca ttaatattac ttcttgtcct gaattatctc
    27721 tggcaaatac aacattatta ttaaatactt tctttataac cataaactac ctccactgta
    27781 aacaaaaaga gacctaaaaa agactaataa tactttaagc atttactaga atttttaggt
    27841 cacgcctgat tacatctatc tcagtaacga tccgtttatt tatctgtatg ataactttat
    27901 tttcattata aaataataac tgtacttagt caatatataa tttcatgtat atctctacta
    27961 gattattgtc ataagtattc actcattcta ctttttttat atgatttata taatctaaac
    28021 atgctaaaat aagttttgaa gttatttcat aacaaaatta aggtcatata ttttttatta
    28081 tatatactcg attatttatc catatataag gaaatccttt attttgtaac tacaataact
    28141 gcctaagttt tgattttctt ttatctttta cttctcctta ttaaaaaata attcttttac
    28201 tgttgtatct aaggctctaa acagtttaat catcaaatca ttgtttggat tattaacctt
    28261 acctaactta ctatatagct ttttaactgt atatctttaa tagacctttt ggtatgctaa
    28321 tccattttat aacactaatt cttgaatcaa gcatatatta cccttacaaa gcatatctta
    28381 taccctctgt agagttatga tagttgatga acctgaagaa attaccctta caagacatat
    28441 ctgatacaac ctcacttatt agtttatatg tttcagaagt atataaataa cacatactta
    28501 cttccaaatt cttaactgtt ttcttagaaa attctaatat gatttcctta catagcgatt
    28561 ataacagtgt cttattagat ttcataaaat tatacatcct ctttacattt aaactttaaa
    28621 tttttatcta ttgtttatta attaactgta tattcactat tttttatcat ttataatact
    28681 cttcttatta tcttaaaaca attataatct aaccttaatc acatattata taaatatttt
    28741 taataaactt aataattaaa taactatatt tcattatcca tcttataaat gtttataaac
    28801 tttccactat tcttcttcaa taaattcaaa caaattttct actgtagtat cgaatacttt
    28861 agcgatatcc atagctaact tcaaagaagg attatatttc ccattttcta agtgtacaat
    28921 agtctcacgt cttaccccaa ccaatttagc aagttcattt tgcgtcataa ggagtttttc
    28981 tctgtattct ttaatttttg ttttcatcaa tattgccact tagttacata tccccctttt
    29041 atcaaagtat atatacaaca ctaatttaga taaagactgt ataaataaaa ctgcaatgag
    29101 gattatacct gtatctaggt ttgatatatt tatattactt gtataaggtg ttgcaaagaa
    29161 aatagatatt attactatag aatatatcat tatttttatt gcaatcttat taacctttga
    29221 tagtataaat tcagatgatt catcaactaa atcattatat tttttattat aaattgaata
    29281 acatccaaaa actattaacc aactcaaccc tggaagagaa gcagtgcttg gacctttaaa
    29341 aatatattta gtagctaata cagctataaa aaaataagtt attacaagta attccaagat
    29401 gagctttgat cttacaccag tttctgattt agacatataa aatcactcct taaaagttat
    29461 atattctcta tattttttaa tttttgtttt cataaatatt attacttagt tacatattcc
    29521 ctttttatca aaatatatat acaaaattaa ttttgacaaa gattgtatga ataaaactgc
    29581 taagagcatt atacctatat ccaaatttga tatgtccaaa gctcttgtat gattaactga
    29641 aaagagaaca gctattacaa acatagagta taacattatt tttgatgcaa tctgatcaac
    29701 ctttaacaac ataagttttg atgcctcatc aactaaatca ttatattttt tattatacat
    29761 gagattgcac acccaaaaag ctaataaaat tataaaagta aaccaaatac cttctccttt
    29821 aaaaataaac ttttgtaata acaagtctat aaaaaaatag gatagcatca ttaactctga
    29881 aacaagattt gccttaacac caagctctga tttagacata taaaatcacc ccttaaaagt
    29941 tatatattta taacacctta tgttataaat atataacttt tccaaataaa ttgcaataga
    30001 atattgaaaa tattcaataa tatcttcata aatatatttt ataaaagatt ttataataaa
    30061 cattacaata cagtactaaa ttagtttatt tcatctatca tctcaatcat atcatttact
    30121 aactattatt ctaatgagca tataacattg aaatttataa ttatacaacc taaatgcctc
    30181 ccaatgtacc ctcaaaaaca tcatttcctg taactgaagt ataattggta tattttatca
    30241 ctgaaataaa aaaacactag tcaataaaga ctagtatttt caagtatttt atcattcttt
    30301 ttttaattat tggagacgaa tcatcagaat aatgcccata atttatctat atgcaaattc
    30361 taaatttaaa ataaagtttc agatgaattt actattctat gctatgatac aacagaaaaa
    30421 actgaaatct atgagtggta gcttcatata atcctagttc ctacagtaat caaaactgag
    30481 gtttattcta tatgtaacta atgataaatt ttattatagg agttattagc tctatttgtg
    30541 ttactataag tagtaattat atatatgata attttttaac acttttctgt tggctatata
    30601 gaaagtggtt tagaatttaa atttaaataa aatttaaatt caccaaaaag aaatctatat
    30661 tagaaaccac tactctattc caccagattg taattttttg ttctttatat cacaatgcta
    30721 taccatatta aattgaaaaa taaattttaa atttatttgt tacaattcat taattcaaaa
    30781 aaatattttg agtcatgagt tttgtaaaga tttagccatg aatttaatgc ttctgattca
    30841 aaaacatcta aaagtttcaa tacttgaaac gtaaattcca acggagcgat tcctgaagcc
    30901 gtaactaaat tttcatcact aactacagat tccgttttat aatatttttc tccagagtaa
    30961 tttggtatta ccattttaag aaactctaag tcattacttg tatgatttct aacatctaat
    31021 agcccttttt tagctaggcc taatgtagct ccacatatgg ctgcaactat agaatcttgt
    31081 attaaagctt ctttaacttt ttctaatatt atctcatgat tcatggataa ccaagtattt
    31141 ccccctggca aaattaataa atccttactt tccatattaa actcatcaac tgtaatttca
    31201 ggtaaaactt ttaacccacc cattgttata ataggatttt tatcaattcc tacagtaatt
    31261 acttttagtg gctttaatcc ttttttaaaa tatcttcctg tatttaattc agcagttaaa
    31321 tatgccactt cccaatctga catagtatca aatacataca tatatacttt tctatttttc
    31381 atctttttta ctccttatgt aattaattta tatatctaat tataaaataa cttaactgac
    31441 atattcagtc agttaagcta ttaaatttga taataatcaa ttaattctag taaatcttca
    31501 attattttct tcctaaggct tagtggttca attacttgta ttgtctttct ataaggtaaa
    31561 agtatatttg gtatgtactt gcatattact tcctcttcga gcataaatat tattttatta
    31621 ggtgttcttt ctttcaagta gtgccctaaa aaccaatgtt ggcttaaact atctaaagaa
    31681 tctatactcc cttctacaac taattgaatt atattttgtt catcttttat ttcaggtata
    31741 attcctttta taaaaaattc acttgctgaa aattctgtcg gtctgttaaa tgtatttgat
    31801 gtttctatga tttcactaat cctatccact ctgaaactac gtacctcatt tctcatatta
    31861 caaaatgcaa ctacgtacca attattattc cagtgaacaa ttccatatgg atttataatt
    31921 ctatattttg attttgcttt acgacctgca tgatagttga tttctacaga tagctccttt
    31981 aatatactat tctccaattt tttaagtact gattctatag acactttccc aattggtttt
    32041 attacttcaa gggctcttaa gtggtttgtt aaaatatctt cttggttttg atttgaatac
    32101 atttttaact ttaatatcga tttatctaat gcttcgtcta agaagtatcc ttctttttta
    32161 gcaaatagag ctgcatgaag tagagatttt tgctcttcta tatcaaaaaa aagaggtgct
    32221 ttagtaaaat tatttaaaag gctataccca ccattatgac ctgtatctga aattataggt
    32281 actccactcg cagaaagtac atcaatatat cggtacactg ttcttatatt tatttctaat
    32341 ttttcagcta tctgttttgc tgtaattttt ttgtcagagt ttaacatcca cagtatagac
    32401 atcatattgt caattttagc catgtagttt cacctctttc tatttgttat ataaagttat
    32461 tatataactt tttgattgat tttttacaaa aaaacattag taaactgcta ataatttata
    32521 ctaagattta tttcaaatat ttatagatag tctttttcta tagttaagtt atccattaaa
    32581 ttacatagta gtatatccat tttagttgat atattttcag ctacctccct tgaatattat
    32641 tacaataagt ctaaatcttc tttcttccat aaatatatta tgaggaaaga ctatcatctt
    32701 taatggttta atagtataaa agatattaat ttaaaaaaat ctatttttat atcagatggg
    32761 tattgttttt tataaaactg aaaaaatcac ctaatagaat ctatgatttt ttaacataac
    32821 ctccataaat tagtaccaat tatcacttca aattcaaatt tttatctgaa gaatatacaa
    32881 tcatatttcc atactcaaca taatattgag tgtttggctt gtatttacaa attattattc
    32941 taacaaaaac aactatacat agattaaaat caccttctaa acataattat taaaatttaa
    33001 ttaatgatat caattccatt aatctctttt aataaaaaat tctcttttct atatgaaaat
    33061 tatttcaaaa cttttaagtt aaatacaaaa atgactataa atagattttt atttacacaa
    33121 atctatttat agtcactata gtttatccaa taaaataata aaataaatat gtacacaata
    33181 aatgttattt acgtcttaag tatttatttg ttagattttt aattaaaaaa aacaagctaa
    33241 tgaacattaa tgctaaatct agcattatat tactttgatc tcctgtttgt acattttgac
    33301 ctacattact atctaaactt tcatctgtat ctatatttat atctggtttt ccattcccat
    33361 ctgtatctat atttatatcc ggctttccat ccccatctgt atctatattt atatccggtt
    33421 ttccatctcc atctatatct atatttatat ctggttttcc atctccatct atatctatat
    33481 ttatatctgg ttttccatct ccatctatat ctatatttat atctggtttt ccatctccat
    33541 ctgtatctat atttatatct ggttttccat ctccatctat atctatattt atattcggtt
    33601 ttccatcccc atctgtatct atatctggtt tgctatttcc tccattatct gggtctggtt
    33661 tactatttcc accgttatct ggatccggtt tgctatttcc tccattatct ggatctggtt
    33721 taccattgcc accgttatct ggatctggtt tgctatttcc tccgttatct ggatccggtt
    33781 tgctatttcc tccattacct gaatccggtt tgctatttcc tccattacct gaatctggtt
    33841 tgctatttcc tccattacct gaatccggtt tgctatttcc tccattacct gaatctggtt
    33901 tgctatttcc tccattatct ggatccggct tgctatttcc tccattatct ggatctggct
    33961 tatttagatt tggattacct gtatcataac ctcctgagcc agtatctaca tttttatcag
    34021 gcttccaata atcatctact ccatcattat ctgtatctat tgctggacga ttataatctg
    34081 tatcagggtt tccatctcca ttggtatcta tatttttatc aggtttccaa ataccatctc
    34141 catttccacc ttctgtacta ggcttccatt cacctgtatt atcagtatct atgttaatat
    34201 ctggcttcca atcaccactc gtatctatat tgatatctgg ccttccatca ccatcagtgt
    34261 ctatatttat atccggtatt ccatccccat ctacatctac atttgtcata tctaacttta
    34321 acttcttttc atcctcacta ccttcaatgt aatttatatc atctccaata tattttacag
    34381 taatagtttg ttcagtagta aaaatatgat gattgctatc taaaggtctc cattcaatag
    34441 atgcagtact ataattatga ctctcactac ccttattatg agtttttaat tctactggat
    34501 ctttatattc ctttccatta atatagaatt gtacgtaacc tcttggagag gcacaatctg
    34561 gtccttcttt cttatctggt gaaatagtaa catttaatgt aaacgtatct gttggtttaa
    34621 aaacatcagt tttagcttta gactcttcta caactaatga agtttctgta tcagcaggaa
    34681 taatttctgc cttataatat gctctatgcc cccaataagc ttcattaaaa ggatatacat
    34741 ttgaaaactg tgttgatgta atgcttaatt gatattttcc agcatcaact tgctttgtaa
    34801 ggtcatcact tcctaaattt gtatctggtt ccattgtttc ctcatttaaa cgttgagaac
    34861 taatagtcat aaatttttta tctgtgagtt tttcaccttt aggagtaatt attggaatac
    34921 ccccatcttt taatatccaa tctaaaaata atttttctac atcttcttca aatggtaatc
    34981 catcatatct tttaataatc aaatatggtg gagtatcatc aatagtaaat tcttcatatt
    35041 gctttaaaaa ttgagaacct gaacctatat ctttaacata aaaatcatct ggttttatat
    35101 ttcctccagt aattttatct ataacaacta aattaacaga tacttcagat cccttaaatt
    35161 gatcagaaaa ccataagtaa agaacccctt gatcatctgt ataactaggt gctccataag
    35221 gataaatttt tccatcaatt agcattttaa aatcatcaac taatgtagta ctaactttat
    35281 cttgtcctaa atactttaaa gatattttat tcataaaaac ttttgttttc ttttctaaat
    35341 caccgtatgc aactgctcca cctaaacttt tgaatgttgt tgcttttaat gaaccacctg
    35401 ttactataac atctcctgtt gtagcactta ggtcaaaaat ccaatctccc actcctgtca
    35461 tataaggtaa aatcgttcct ccagttacaa gtatacgata tcctctattt gttcctggat
    35521 taacacatcc atccccaaat gcactaccat gtgcacctcc atatgaatag gaaagtccac
    35581 cattaattgt aatatttcca ctataaccag aacctgtgct aggatctact actgagggac
    35641 ttaatgcatt ctttgttgtt gtatagtatc ctccacctat tccagctcca tggtatgcac
    35701 ctttagcagt tatagtacca ccattgattg tgatatctcc accactacca ttaccgcctc
    35761 cacctattcc agctccatat cctgtattac gactcgattc ttgacctata gcagttatag
    35821 caccaccatt aataataata tttcctccac tttcagtatt tccaccgcca atacctgcag
    35881 aataataatt tccaacaact tttaaacttc ctctattatt actatctagt agtgtaaggc
    35941 gatctccttt ttttaacttg tctccatttt ctagagttac atctctagga atttttccac
    36001 cctcaggaac tataaactct ccttcaatat ctctatttac aacagaatca tcaatagtta
    36061 gagtagtacc ttttcctgct ctcatacctg gataatatac atcacctgac tctagcttgt
    36121 tttcactacc atcttgtaat ataacatgtg catatgcacc accatcttta tctggtgtta
    36181 aagtaaatgg gctattagat gcagttttaa tatttactcc atcaaatatt aattcagctt
    36241 ttacatcttt aggtataaca atcctatgtg ttgttggagt atttggatta ttattttgaa
    36301 ttgtcatttg ttttccacta agaatagtta aaacgccact tgcaaattta aaatcgacat
    36361 ttatttctcc accactaatt ttaaagtctc ctacagatgt aggatcttct gcagtggctt
    36421 cttccacaac taatgcattc atatcttctt ctgcatttcc tgcattagaa tcttcttctg
    36481 catttcctgc attagcatct tcttctgcat ttcctgcatt agcatctcct tctgcatttc
    36541 ctgcattaga atcttcttct gcatttcctg cattagcatc ccctcctgca tttcctgcat
    36601 tagcatcccc tcctgcattt cctgcattag catcccctcc tgcatttcct gcattagcat
    36661 cccctcctgc atttcctgca ttagtatctc cttctgcatt tcctgcatta gtatctcctt
    36721 ctgcatttcc tgcattagta tctccttctg catttcctgc attagtactt tcttctatag
    36781 tatctgcgaa agtatttgac gcattaaatg atgctactga tccaagtccc attgagatac
    36841 ttaaaatcat tgatagtgct ttctttttac atttttgttg cataaattcc ccctcccctt
    36901 aataattttt aatatacact gtatacatcc ttatatacac tctaataatt cttaatatac
    36961 actgtataca tccttatatg cactctaata atttttaata tatacttaaa acttatattt
    37021 gtcaatgaat atactgtttt tttcaacctt atatattata agtattcatt atttgctaaa
    37081 taactacaat aaaaatttta attatataaa atttatttaa ttttttatat aataatacta
    37141 aggggtaatt aattgaaaaa ctagtaaaaa tattaataaa tattatttgc attattgcgc
    37201 taactttctc tagcctttcg atttacataa aattatctga atataaaaaa gctgatgaag
    37261 tatatacgga actcagagaa aacactatca ataatagcaa acatcaagaa ctttatgata
    37321 aaaataatga ttatagattc tggcttaaaa taaacaacgt aaatataaac tatcctgtag
    37381 tacaaggata taacaatgat ttctacctaa ctcatgattt ttataaaaat tatcttcctt
    37441 taggtagtat tttcatggat tatagaaata attttgaaaa tgataagagt ttaatagtct
    37501 atggtcatta tatgaaaaat aaaactatgt ttggtcaact agaaaactat actgatgaag
    37561 tatttttcaa agaaaataac ttagtagaaa taaattataa agtacaaact tatacttatg
    37621 aaattttttc agtatatact gctgacttaa ttaacagaga ttatttaagt atacacttta
    37681 ataataatga tgagtttaaa tattctttga attatataac ctaaaaatca gtacacaaat
    37741 ctgatataaa ggtagatatt tcagataaaa ttataacttt gtatacttgt aactatgaat
    37801 ttaaagatgc tcttactata gttcatgcta aattaatttc aaaaaagaga cctataagta
    37861 ggtttgtgta aatgcagact tatctacagt cttttttgaa cgtaaaattg gtaagtatgg
    37921 aataatattt atattaaact tttgtaggca aagaaatgaa aaatcaaaga gaaataatta
    37981 ttacagattt agattaccaa gctgaattaa gaaagtgtaa aactatggag gatgtagttg
    38041 gtgaagatag tatataatag cttaacttat aaattatctt ggtgatatta atgaagtttc
    38101 aaagtatata ttatttattg atataaataa aattaaatct gtttctaata gatgtacctt
    38161 tgtttggaga caaactattg aagaaatata actaaaatgt tgaagtagct acctgacttt
    38221 attttgaaat gcgaacaaga ttttggcatt aagataattt agaaagaatc actggtacac
    38281 aaggatgtga acttagaatg aatagaagta ttcaggcata aggagctttc acccaaataa
    38341 aacatagtat gaactttaaa agaattctaa tcaaaggcaa tcaaaatatt ctttcagagt
    38401 ttatacctgt agtgttagcc tatagtattt ctaagtttca caaataagat aatcattaat
    38461 aaatgtagta tgtatattca tcctctcaaa aatatttttt aatcttacac tttgtaaatt
    38521 attctatttt aaaattgtat ttttatacct ttatttctta tgaccgtttt caagctcaaa
    38581 ttttgcataa atataaatta atgaaaagca agtaaaaaaa gaactgtcgc acagcaattt
    38641 aaaattactc attcgacagt ccttatttcg tggattaatg ttggataaga atcacaagaa
    38701 taatacccac tattttattt gaactatcca tctagtgctt caaacacatc tctaagatac
    38761 tcatttggtt catcattaaa taaatttaat atcttttctg ctactactcg acctaataaa
    38821 ggtggtactg catttcctat ctgttgatat acagaatctt ttgtacctaa aaacttataa
    38881 ctatcaggga atgactgcaa tctagccgcc tctcttgcgc taacagctct gtttttaata
    38941 ggatgtatcc acatagattt tcttacattt acaacagtat ctgatggctt atcatatact
    39001 agtcttttat aaatagtatt ttgagttctt tctggatcag cataagttcc ttttaactca
    39061 tctggcaaag aatgaaaatt atttccttga tttatatatt caaatctttt tagtgcgata
    39121 tttcttgtat ctgtacatac atgattgtat attttattgc tatccaatat tatctttcta
    39181 taaaaatctt tttcaatatt attactattt ttttcaaaac aataatccat actaccttta
    39241 gaagcctcat attcagctaa atcgcctatt gcatctctta ctgtaacata attttctcca
    39301 actataggct taggagtttc tatcttttta ttttttaaga agtctttttt tgctcctatc
    39361 aatacaaatc tttctctatt ttgaggtact ccataatcaa ttgaatttag tgcacttcca
    39421 tttaatacat atcctagata ctcaaaggat gcatttatat aatctattat aatgtaagta
    39481 tccattttag ctataatttt attatcatca agtgtatttt cgaaaattac agattcagat
    39541 tttaactcat atattcctaa aaataaattt tgaatatcca agaataagtt caaataaaat
    39601 atttgttcat ctgataaatt tctattatat aacatatttt ctaatatatt ttttacttta
    39661 tttattaatt ctaaatacca ttttggcatt tttccccttg tttccaattt t
//
LOCUS       DAMKMI010000020        38892 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMKMI010000020 DAMKMI010000000
VERSION     DAMKMI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38892
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.020"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(287..1126)
                     /locus_tag="PC429_000985"
     CDS             complement(287..1126)
                     /locus_tag="PC429_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3846502.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1150..1908)
                     /locus_tag="PC429_000986"
     CDS             complement(1150..1908)
                     /locus_tag="PC429_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3846503.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1955..2428)
                     /locus_tag="PC429_000987"
     CDS             complement(1955..2428)
                     /locus_tag="PC429_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846504.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2457..2873)
                     /locus_tag="PC429_000988"
     CDS             complement(2457..2873)
                     /locus_tag="PC429_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846505.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2920..3972)
                     /locus_tag="PC429_000989"
     CDS             complement(2920..3972)
                     /locus_tag="PC429_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF3846506.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4368..5048
                     /locus_tag="PC429_000990"
     CDS             4368..5048
                     /locus_tag="PC429_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF3846507.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5181..5501)
                     /gene="sugE"
                     /locus_tag="PC429_000991"
     CDS             complement(5181..5501)
                     /gene="sugE"
                     /locus_tag="PC429_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF3846508.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5883..6710
                     /locus_tag="PC429_000992"
     CDS             5883..6710
                     /locus_tag="PC429_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF3846509.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7532..8206
                     /locus_tag="PC429_000993"
     CDS             7532..8206
                     /locus_tag="PC429_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846510.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKGALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREKRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8194..9354
                     /locus_tag="PC429_000994"
     CDS             8194..9354
                     /locus_tag="PC429_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3846511.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASTLTTFSYILLNNLYNSIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9625..10098
                     /locus_tag="PC429_000995"
     CDS             9625..10098
                     /locus_tag="PC429_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF3846512.1"
                     /translation="MLSFTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            10384..11052
                     /locus_tag="PC429_000996"
     CDS             10384..11052
                     /locus_tag="PC429_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846513.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            11055..13523
                     /locus_tag="PC429_000997"
     CDS             11055..13523
                     /locus_tag="PC429_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846514.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFATIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDVNL"
     gene            complement(13762..15186)
                     /locus_tag="PC429_000998"
     CDS             complement(13762..15186)
                     /locus_tag="PC429_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3846515.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15205..15897)
                     /locus_tag="PC429_000999"
     CDS             complement(15205..15897)
                     /locus_tag="PC429_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846516.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15952..16728)
                     /locus_tag="PC429_001000"
     CDS             complement(15952..16728)
                     /locus_tag="PC429_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF3846517.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(16725..17495)
                     /locus_tag="PC429_001001"
     CDS             complement(16725..17495)
                     /locus_tag="PC429_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF3846518.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17492..18196)
                     /locus_tag="PC429_001002"
     CDS             complement(17492..18196)
                     /locus_tag="PC429_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF3846519.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18451..18963)
                     /locus_tag="PC429_001003"
     CDS             complement(18451..18963)
                     /locus_tag="PC429_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846520.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19289..19546
                     /locus_tag="PC429_001004"
     CDS             19289..19546
                     /locus_tag="PC429_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846521.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19689..20315
                     /locus_tag="PC429_001005"
     CDS             19689..20315
                     /locus_tag="PC429_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF3846522.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20562..21341)
                     /locus_tag="PC429_001006"
     CDS             complement(20562..21341)
                     /locus_tag="PC429_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF3846523.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNRCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21409..21870)
                     /locus_tag="PC429_001007"
     CDS             complement(21409..21870)
                     /locus_tag="PC429_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3846524.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21846..22523)
                     /locus_tag="PC429_001008"
     CDS             complement(21846..22523)
                     /locus_tag="PC429_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3846525.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22756..23142
                     /locus_tag="PC429_001009"
     CDS             22756..23142
                     /locus_tag="PC429_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3846526.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23145..24944
                     /locus_tag="PC429_001010"
     CDS             23145..24944
                     /locus_tag="PC429_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF3846527.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSSNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILKDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25299..27173)
                     /locus_tag="PC429_001011"
     CDS             complement(25299..27173)
                     /locus_tag="PC429_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3846528.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27178..28842)
                     /locus_tag="PC429_001012"
     CDS             complement(27178..28842)
                     /locus_tag="PC429_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF3846529.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(28856..29677)
                     /locus_tag="PC429_001013"
     CDS             complement(28856..29677)
                     /locus_tag="PC429_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3846530.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29776..30540)
                     /locus_tag="PC429_001014"
     CDS             complement(29776..30540)
                     /locus_tag="PC429_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3846531.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30537..31286)
                     /locus_tag="PC429_001015"
     CDS             complement(30537..31286)
                     /locus_tag="PC429_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3846532.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31790..32395)
                     /locus_tag="PC429_001016"
     CDS             complement(31790..32395)
                     /locus_tag="PC429_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3846533.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            32574..33212
                     /locus_tag="PC429_001017"
     CDS             32574..33212
                     /locus_tag="PC429_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3846534.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33344..34006
                     /locus_tag="PC429_001018"
     CDS             33344..34006
                     /locus_tag="PC429_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846535.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            34006..35187
                     /locus_tag="PC429_001019"
     CDS             34006..35187
                     /locus_tag="PC429_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3846536.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(35368..38028)
                     /locus_tag="PC429_001020"
     CDS             complement(35368..38028)
                     /locus_tag="PC429_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846537.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(38025..38708)
                     /locus_tag="PC429_001021"
     CDS             complement(38025..38708)
                     /locus_tag="PC429_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846538.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
BASE COUNT        13744 a         6342 c         4800 g        14006 t
ORIGIN      
        1 aatatataac taatatgtta taatatttta attaaattaa ataaatttat atttttttga
       61 aatcacttaa tattttatat aaaaatgaaa aagcacctca ttctgattaa aaatctttct
      121 gagatacttt ttaaaatatt aaatactaca tatagcttac ataagtttaa aatttaatct
      181 attataaaat taccctaatt gccaaacttt ttaatataaa ttttccaaca tcctacaaat
      241 ataatcaata gatatagaat cctaacttac accaatatat atttatttaa aatagtccta
      301 aaatacctgt aatagctccc actactatca gtataaacat tatttttatt ggagaaagac
      361 ctttttttaa agcaaagagt actaataaag taagtaccaa aggaattaac ccaggaaata
      421 tcttatcgag taaatccgtt tgaagactaa atttaaaatc accactagta taacttattg
      481 gtgtagataa attaataaac ctacctacaa gtccccccat gactaaggta cctagtatag
      541 aagccccttc tataacctta tttactattc caccttgaag tatactttca acggcttttt
      601 tcccatactt atatccattt gtccacattc caaaagaaat tgaagtcatt attatatatt
      661 gagctattac aaataatata ggtcctgcaa ttgatgctcc tccctcagtt aaaccaatac
      721 aaacagccaa cactatagga gttataactc cttgagttaa agtatcgcca attcccgcta
      781 gtggacccat caagcctgtt ttgatagcat tcataccatc tccacttatt tcatctgctc
      841 cattagcttt ttcttcttcc atagctattg ttattccatg tataacacat ccacatatag
      901 gttctgtgtt aaaaaatgct agatgagtct ccacttcttg ttttaactct tccttatctg
      961 gatataattt tttaagtact ggcaacattg aatgtgaaaa agctgttgat tgcaatcttt
     1021 cataattgta attagattgt gcaaagaaaa accatcttaa ccatgatttt attacatctt
     1081 tcttgcttaa cgttttctta gatacttcat tttttatatc tgacataata ttttccccct
     1141 cacatgtatt taagatgtag cactatcttt tccgtactta attgaagtaa ttgtaagagc
     1201 aagtattact ccaataacag atattactat tatatctact ttaaaataaa cagccattag
     1261 tattcctaat atgaaaaagg gcatagtaaa cttgtttcca atcaatttta aattcatagc
     1321 aatcccaagt gctggcaaca tagccccaat tacattaaat acatgtaaca cattatcatt
     1381 cattacttcc aacataccat ctatcgcatt tggaccaaaa taaacaacta ataatgctgg
     1441 tataaaactc attgcaaata acagtatctg tggtggtact atatttataa atgctactct
     1501 atcaacattc cctttttgaa cctctctatc agcccaatgt gcaaaaaaag aatttactgt
     1561 catctttcca atccaaagta cagttgcaac agttcccaat ggtacagcta ttgccattgc
     1621 agtacttaca tctagcttag atgctatagc taaagcagta cctacatatc ctgcaaaagc
     1681 tgggtccccc atctgagctc cccctgctga tataaaacct agatatatca tatttatacc
     1741 tgctcctata attgctcctt gaacagggtc tcctaatata attcccgtta caaatccagc
     1801 caatagaggt ctattccatg taaaaaatcc aacataagtc aaccacggac tccaagaaag
     1861 ataataaaaa attgctatta atactgcttg aattagtgaa atgtgcatat tcttcctccc
     1921 tattttcttt taaagattta caagatagtc ttctttacaa taatgttcct acatcaattt
     1981 tctttgcatc tggtactatt tgtataaagc atttaacatc ttttgatatt agctctctaa
     2041 atatttcttt ttcttcatct gacacagata tatttttata aaaacttttt ctaccaagcc
     2101 ctgctcccat acctccaaca ttcaattcct ttaaatctac cccgctattt actaaaccta
     2161 agacagtttt agggtatttg accaatacta ttaaattttc atctccatta tatgatttta
     2221 agacttcagc acctgattct acatcatgta cttcaacctt aacagttgat ggagctgcca
     2281 tttgtaaaac ttgaactata aatgggtcct ttactactaa atcatccacg attactatcc
     2341 tttttgctga tgtaatctta caccaagcag taataacttg cccatgtatt aatctatcat
     2401 caattcttgt taatgctata ttactcataa ttattcctcc agatttttaa tttattttac
     2461 aactttaaaa cagttttaac atctttgatt cctgatatcc caacttcctc acataaattc
     2521 ttcaagtctt ttaacttcat agtatctctg tttaaaaata actctaaaag cataggtaaa
     2581 tttacacctg tcaaacattc taactcacat accttagcaa atttatttat tataatagca
     2641 gatgtattaa atggagttcc gccataaaaa tcaagcaata ctaaaacccc ttcatcctta
     2701 tatttctcaa ttccatcttc taactttcta ctaaactcat ctatgctgtc accatggttt
     2761 aatcctaaag tttctacatt ttcctgtttg cctactatta attcagcact ttctaacatt
     2821 gctttagcca tatttccatg tgtggctata attattcctg ccatatgaat catacctttc
     2881 ttaacctttt ttatatggat tattatttag tactctaatc taattatcta tattaatcat
     2941 tatcttacca aagaaaatat cccttttgta tactttttca aaataagctg gtacttcttc
     3001 taaatttatt ctatgtgtta ctaatttttc aacattcatg tcaccctttt gcatatgata
     3061 caatccagca ctccattctt ttcctgggaa tggaaaggag tttccaaacc aagttccctt
     3121 tacagttaat tcacttctta ctatcttttc aaattgctct ctagtcaaag ctacatcccc
     3181 atatggtaca cctgcataca atactgtacc tcccttttta gctagcaaca gtacttgacc
     3241 acatgtcagt ggagtaccag ccgattcaat aacaatatct gcaccatctc catcagttaa
     3301 cctttttatt tcttctacaa tattcttttc ttttgcattt atacaaatat ctgcccctaa
     3361 ctcttttgcc aagtctaact tctcatcaaa tacatccaca gctattattt ttgtagaacc
     3421 aaatattttt gcccattgta ttgaaaataa tcctattgga cctgtaccaa gtactacaac
     3481 agtatctcca actttagctt ctgacctaaa aagaccatgt cctgctatac atactggttc
     3541 tagcgcagcg gcagtctcat aacttatctc atctggtatt tttattaaat ttctctcttt
     3601 cacctttgta tactctgcaa aacaacctcc aagttcttta tttcctatta ttgctacatt
     3661 attacatctt gaatacaagc cttttttaca ttcatcacat tcaaaacatg gcatagctgg
     3721 acaaacagca actctatctc ctattttaaa actccttact tctttaccaa cctgtgccac
     3781 ttctccagaa aattcatgcc ctaaaatttc tccaaccata tgtggacctg tctttgaata
     3841 ttttgatata tcagaaccac atattccagc tacttttact ttaattatta catcatcttt
     3901 ttctaatatt tttggtacat ccacatcctc cactcttgtg tctcttatac catagaatct
     3961 aactgacttc actattaacc acccctactt tttatttgag aatatatccc tattgtttat
     4021 atgttaaata tctaaagcaa ctttcatgcc aatatatgta agcattgtat tcatgcattt
     4081 tataaaatta tcattaaatg tgtgtcaaag tgtgtcaaaa gtttcttata ttaaaactta
     4141 tgtgtgtcaa tataaacgac actaacattt tatattagtt ctcttattat aagttttata
     4201 aaatgtactt tttttgttat ttttataaaa ttgttcggaa atttcccttc tatttgtgat
     4261 ataatattaa atataaggta tcaatataaa ttttcatgcg aatatcagaa cttttttgac
     4321 actaatgcct tattcaatat aataatttaa ctttttggag gttgtttatg aaaaaagtgt
     4381 atcaaggaaa gactaaagat gtttttgaat tagataatgg aaattactta cttaaattta
     4441 aagacgatgt aactggtaaa gatggagtat ttgacccagg cgaaaatagt gtaggtctaa
     4501 gtatagatgg aattggtaga gctaatttag agacatctgt taagttcttt gaaattctaa
     4561 acaatgctgg aattaaaact cattatgtaa gtgctaattt agaagaagca actatggaag
     4621 ttctaccagc aaaagttttt ggtaatggat tagaagttat ttgtcgttat agagctgttg
     4681 gtagtttctt acgtcgttat ggttcatgtg ttgaagaagg tgctaaacta gattgttacg
     4741 tagaagcaac tttaaaagat gatgaccgtt gtgaccctct tataacatct gaaggtttag
     4801 aagctttaaa cattatgact caagctcaat ttgattcaat gaaaagcatg actcaaaaaa
     4861 tttctaacat agttcgcgat actatagctg aaaaaggtct tgagctttat gatattaaat
     4921 ttgaatttgg attctacaat gatgaagtaa tcttaataga tgaaatagca tctggaaata
     4981 tgcgtgttta taaagatggt gtaatagttg accctatgga tttaactgct cttttattag
     5041 ataaatagta tatacatata atttacaaat ataataaact tataaataag ataagctatg
     5101 aagtaaagac tttttaaatg ttgattattt ttaaacattt tctcgccttt aatttcatag
     5161 cttatttaaa ttaatatact ctaattagta gataatagct ttaaccctac aattcccacc
     5221 acaataaaac ctatacatgc aattcttgat atactaatag gctcatttaa aatagccatg
     5281 cctacaatag ctgtaccgac ggtaccaatt ccagtccata ttgcatatga agttccaagt
     5341 ggaagatgtt ttagtgctaa tgacaaaaaa taaaaactta caatcattcc aacaacagta
     5401 agtatacttg gagttaattt agaaaatcca tgtgaatttt ttagttgaaa agcccaaaac
     5461 acttcaaaca aacctgcaac aaacaaaaat aaccatttca taaattcatc cctcttttca
     5521 aatatgtctc ttatttttcc aataaaataa cctgagaaat attataaaat atctcccagg
     5581 tttttgtcct tccgtgttca caacaaaaac tatgttgtgg gttttatctc ggaccagacc
     5641 aatcattaat aattgcggaa ccctataaaa ctctattaat tattctattt tatctataat
     5701 aatactatca acatatataa aaaaagtcaa tatatgggtt ccatatataa taaaaataac
     5761 ttttatatac cttttaatat tcttattcac ttctctccat acccatttgg gtcaatctaa
     5821 gaatatctaa tttttattta atatgttata ataacataaa aatattgaaa ggagtaattt
     5881 ctatgaattt ctataaaatt gcagtatgtc agatgattac tacagaaaat aaaattgaaa
     5941 atatcaatca tgctgtagat atggtaacag aagctgccat caatggatct aaaattgtag
     6001 ttttgccaga aatgtttaac tgtccatatg aaaataaata ttttcctaaa tttgctgaag
     6061 aatatccagg tgaaacaaca acaatattga gtaaattagc tgagaaacat ggaatttatc
     6121 tagtttctgg tagtattcct gagttagaag atggaaaaat ctataatact tgctatgtat
     6181 ttgataaaaa tggagctcta attggaaaac atcgtaaaat gcacttattt gacattgaag
     6241 ttacaggaaa agttagtttt aaagaatcag atactcttac agctggaaat gatgttactg
     6301 taatagatac cgaatatgga aaagttggta tagctatttg ttatgatatt cgtttccctg
     6361 agttatcacg tttaatggct cttaaaggtg ctgaaatcgt tatcttgcct gctgcattta
     6421 atatgactac tggtcccgcc cattgggagt tatctataag aatgcgtgct ttagataatc
     6481 agatattcta tgttggtgct gcacctgcta gaaatatgaa tgcttcctac atagcttttg
     6541 gaaattcaag aatttcagac ccttggggaa gaataattgc tcaagctgat gaaaaagaat
     6601 gtattattta tgctgacatt gatagagatt taattcctga cataagacaa cagttaccac
     6661 ttcttaagca cagaagaact gatttatatg aacttaatac tttgaaataa aacagaacat
     6721 aaccccataa atcataaatt aagtgttaaa aaaatttatg gggttctcat taatttgatt
     6781 taaaattaat ttcataaaaa taatactaat attaatttaa ttaaataata ctaataatta
     6841 ctaatgatta tgctaattat cagcatttac taatttaatc actattagtt atttctttta
     6901 ttaaatatct ttactttttt tacttattat gtcttctagc tttacatttg gattatagta
     6961 tttctttcta tttttagtca catctttcta ttgaattact tgcttaaaac accaatgcac
     7021 acattccaga caatgttcac aattatgtaa aaatcttgct ctaccttctt caatgttgat
     7081 attatctctc ctagttgata ttatctttcc taaaatttct tttaattaaa aaacaacatt
     7141 cgttgatata tcgagtatat tttaagcaac tgaaaaaaac aataataatt gatatatcaa
     7201 ctaattaatg tagattaatt catttttata atttatatac tttttaatta atctaccttt
     7261 ttaattaaaa ttcttccttt ctatttatcc attttaaact taaattataa gatttcatca
     7321 gctgtgtttt tgcattccaa taagtattaa atatcaaata tatattctat taaaatttct
     7381 aataatttca tccttgattt atttaatata taaaaatata ctaactttaa tgattaagtt
     7441 ttgaaatata actaagaatt tgagatttta ttgagatttt tatactaaag tatacatact
     7501 atacaagctt aatttagaag ggagcattct tatgaacaat aaaatactcg tcgtagatga
     7561 tgaggaaggt ataataacac tcttaaaaga ttactttgaa atgaatggtt atgaggttta
     7621 cactgctcaa agtggaaaag gcgcattaaa aaaaatatcc aaaaatccag atattattct
     7681 acttgatata aacatgcctg aaatagatgg aattgaagtt tgtaatagaa ttagagatta
     7741 tgtatcatgc cctattctat ttcttacagc tagaattgaa aatactgaca agataaatgg
     7801 ttttcgtgct ggtgctgatg attatattgt aaaacctttt gatttagacg aacttggagc
     7861 tagagtatct gctcatttaa gacgtgaaaa aaggaagtca aatcaaacta aaattaaatt
     7921 ttataatgat ttaacgatag attacgaaaa aaggttaatt tgtatagata ataatcagat
     7981 ttcactttca aaaaaggaat ttgatatagt agaacttctc tctatgaatc ctggacaagt
     8041 atttgataaa gaaaaaatct atgatagagt ctggggatac aatagcaatg gaaatagtga
     8101 tgttataatg gaacacatta gaaagatacg aatcaaacta tcaaaacata ctctggaaaa
     8161 ttatatagaa acagtctggg gagttggtta taaatggata aaataaataa tatgtcatta
     8221 aaaaaagctt tctttgttct tactttatct tcactaatta tagcatcaac acttactact
     8281 ttctcataca ttttgcttaa taatctatat aattctatac aagataagta tttaaaaaca
     8341 cctataaata cgggttttat aaccatagtt caaaacaatg tgaaccaatt tagtgattat
     8401 gataaaacgt tacttaatat aatcaacata cttcagcttg tacttccact tatatttttt
     8461 ataggacttt tattattagc tgatataatt ttttacaagg taaaattaaa aacacccata
     8521 gaaatactca ataaaggtgc aatggaaata tccaacaaca atttagactt ttgcttagag
     8581 tataataata atgatgaatt aggtaatcta tgtaatgctt ttgaaaaaat gaggtctgaa
     8641 ctaaataaaa acaacataaa gatgtggact atgattaatg atagaaaaca acttaatgct
     8701 gctttttctc atgatttaag aaatccccta acagttctaa aaggttactc aaactattta
     8761 acaaaataca taccaacagg taagcttagt gatgagaaga ttttatcaac cacacaactt
     8821 atgtctgagc atattaacag aattgaatac tatgtagaaa atatgagtaa tgctcaacga
     8881 ttagaagatt tagttgtttc aaaatctatg tcgaatataa acaagtttat agaaaactta
     8941 gatgaaaata tatctatcat tgctaaacaa gaaggaaaat cttttacact taaaagtcaa
     9001 ataaataata taaatctatt ttttgatgaa aatatcattc ttagagtcgt cgaaaatata
     9061 atatcgaatg cttttagata tgctagaaat aatgtatcca tacttatata tcttgaacaa
     9121 gaactcctta cctttgtaat tgaagatgat ggtataggat ttagtaagga atctctaaaa
     9181 ttagctttaa gacctttcta tagagataag actttaaatg acagcaatgc acactttggt
     9241 atgggtcttt atatctcaaa aatattatgt gaaaaacatg gtggctctat ttctattgaa
     9301 aataattcta ctaacagtgc taaaattact gctaaattct caacaagaaa ttaaaatgat
     9361 tttgtaagct ttcactgcta ttatataaaa tttgttagtt tagaatttat aaaatgtatt
     9421 tcaatttata accaatatat atactatttt gtagaaattg taagagttgc ttcaattata
     9481 atattttggc aaaaggcatt tgaaacaata atagaaatat atatttaaat atgtagaata
     9541 gtatgaaatt agaattttgg aggtgattat attaaaagtt ttgctaataa tattacattc
     9601 tctcgtattg tgtttgcatt gctaatgctt agttttacat tctgttcact gcctttctgg
     9661 atatgttatt tttggtgtgg aattagtgat atatttgatg gtcttattgc ccgaaagttg
     9721 agacaacaaa gtggaatagg tgcaaagtta gatagtattg ctgatttcat tttttcggtt
     9781 acaattttgg ttactgtcgg aaaaaatatt gctcttccca tgtatctatg gtggtgtatt
     9841 gttgcaatag cattgttacg gttgattagt tacagtattg gtttttttaa gtatcgcaca
     9901 ttttcatcgc ttcatacttt gatgaataaa atcactggaa tttctatctt tgcgtttcca
     9961 ttactctata tttgctttgg tcttgatgtt gcaggaatta tgatatgtat cgtggctttt
    10021 ctttcttctt ttgaagaaat agcaattatg ataaaatcaa aagaattaaa tcgtgatata
    10081 aaaagtatat ttatgtgaaa acttagaatt tgtgacaaaa ataatttata taataatgag
    10141 gaagtaaaac ataaaaaatt atccacaagt ttatgtgtaa aatagtttta aagcaataga
    10201 gttgtattgt aaagccttta gtgcataaat tacattttaa attaaaaatg atactgaaac
    10261 tgcttatcag cactgcgaat tatccgcaaa tagtgaagac tttttagatt taagctaaaa
    10321 gttgggaaaa aattgagttt taaaacttac actcttctta tatagaaaag gagagtgttt
    10381 tttatgatta taaaagcaaa acaattatca aaaatatatg gttctaataa caataaagtt
    10441 atcgcactta ataatgtaaa tttagaaata aactcaggtg aatttgtttc tgtaattgga
    10501 ccttctggaa gtggaaaaag cacactttta catattttaa gtggattaga taatcctacc
    10561 tctggacaag tgttacttga tgataaagat atatataaac acactgaaaa agaactatct
    10621 gcacttagaa gaaaatcctt tggattcgtc tttcaacaat ttaatctttt acctgttctg
    10681 acagcatctg aaaatatttc aatgcctgta cttctagata aaaaacaacc tgataaagaa
    10741 tatttaaatg aaatctcatc attacttgga attgcagata gactcaatca tcttccccat
    10801 gaactttcag gtggtcaaca acaaagagtt gcaattgcta gagcacttat tgcaaaacca
    10861 gatataatat ttgctgatga gcctacagga aacttggaca gtaaaagtgg cagtgaagtt
    10921 atgaatcttc ttataaaaac ttcaaaacag tttggaaaga ctttagtggt aattactcat
    10981 gatgatagaa ttgcaaaact tgctgataga aaaatttcaa ttattgatgg tgttcttatg
    11041 gaggtgaaat agaaatgaag aattaccttt cactttcaat aaaagaatta aaaagccaga
    11101 aacttatgac tacattcatc attatagcaa tagtactatc aactattatg acaactgtgg
    11161 ttggacagtc aattggtatc ctacaaaatt taagaattga acaagcccgt tcttttaacg
    11221 gtgatagaca tgtatccttt caccaactta ctaaaaatca agttgatgac ataaagaaag
    11281 atgatagagt ttatcaagct ggtacttcaa taactcttgg cagtagtaaa ataaaggatt
    11341 ctggtatctc tgtacttgta aaagagtatg ataaaacagg actttccaac taccccaaat
    11401 taatgaaatt aaaatctgga aatttgccta aagataaaaa cgaaatagcc ttagatgaaa
    11461 acacactaaa gcttatgggt ataaaaccaa gactaggagt gactatacct atgaatttag
    11521 atatttcact tttaaatgat acaataccac catataatta cacagctaac tttaaattga
    11581 gtggtatttt agaggatgat tatacaggat atgtaagtgg tatagtaaac ggtattgtag
    11641 gcaaaggtgc atcagaaaat ctattaccta agagatatat tttatcctct ttggatttta
    11701 aaataaaaca gcaagagaag tttcaagaaa tagtaaatca attagctaaa aaaataaatc
    11761 tttctcacaa ttcaattcaa tataattgga tttacttaaa tgctcttaaa atacagtttg
    11821 aaaaagatga aaatagttct aatagtgatg gtatatctat gattatattg gtatctttat
    11881 ttgttgcctt attggtttta ctggcttctg gacttgtaat ctacaatatt ttaaaaatct
    11941 ctgttacaaa gaaaattaag gagtatggat gtttaagagc aataggagct gaaccaaacc
    12001 agatatataa gatagttatt ttacaaatct taattttatg tactattgcc atacctattg
    12061 gagctgtaat tggaattata tcttctaaag gcataactgg aatggtcact aatatcttaa
    12121 atccagatat acttctagca aatgataata aagaaattac tgagcttata cataaaaaca
    12181 caacagcata tatattccca ttagtattaa gcactagtat atctttgatt ttttcactta
    12241 tttcagcatt accatcagca atatatgctt cacatgtctc tcccaaaatt gctatggctg
    12301 gcagtactac caaaatcaaa agaaaaatta aacgagaaaa aacaataaaa aactttgaaa
    12361 gacatctagc ctggctaaac ttaaaaagaa ataaaggaag aactataata accatactat
    12421 cattatttat gagtataaca gtattcgttg cactgagtga gttctctaat gtattagatg
    12481 tatctaggtc agtaagtaat ttaaaagaag gagatttttc cttaacaaat gaaatatctg
    12541 gttttgataa gtcaatacta gataaaatag ataaaatgaa aaacgtcaat cgcacttcct
    12601 ttataaagta ctctgaatac aaacagggcg aaatagatac agatataaat tttgaaaata
    12661 gtggtgagat gttaaaaatc attggaattg atgagcaaac tttaaaagac ttaatgcctt
    12721 ctattacaga ttctatacta gaagacttta aaaatggttc tatctgtttt ataaaaaatc
    12781 cgattgctat ttctacacca ggtgtaaaaa ctagacatac aaatttaaaa cctaaagata
    12841 atatcacaat taacaaaaaa caactagata tatatagtac agtagataaa atgttctttt
    12901 tacaaggtaa tggctggata aatggtgttg atgttattgt atatgattct gtttataaca
    12961 ccttgacaaa taaaaacaag ttcaaccaga taaatattta tgctaaagat aaatcaaaat
    13021 tagaacaaat aagattgtct attgaacaga tttgtgaaaa taatcctggc tctcattgga
    13081 tatcatatat tgaatcagat aaacaactta aagagagttt taaacaaata gaatttctag
    13141 catgggcagt tatattattt gtgggattga ttggtacttt aaacatcatt aacacaactc
    13201 atacaaatat aaatactagg actaatgaga ttggagttaa aagagctatt ggtatgagta
    13261 atagtagctt atataagatg tttttatggg aaggtgttta ttatggaatt tttgctacta
    13321 tatttggttc aattgctggg tatgtcagtg ctattattat aaacatggca actatagaaa
    13381 agcttgattt tactaatata ccaattactt caattttaca agctactatt atttctatac
    13441 ttgcatgtat tatagctact cttattccgt taagaaaagt taaaaagatg aatataattg
    13501 actgtataga tgtcaattta taactatttt acagataata aataaattaa attatcaatg
    13561 actgtctcta ttttctttta aatctaacta ataaatcttt ttaactataa tataagcttt
    13621 aaaaaaggcc tgtctcaaaa tagagataag ccttttttca ttgtcgaaat aatgaaaata
    13681 tatttaactt cacaaaaaaa gagtgcctca cgaattaaaa atttcgcttt gagacactcc
    13741 cctttaattt aaaatatata gttaaatgct atgccaaaat ttcacacatg ctccacctga
    13801 ttttgaattt tctattttaa cagaacctcc aagctgttcc actaattgtt ttacaatgta
    13861 aagcccaaga ccagaatgtc ctcctttagt ctgcctagct tcatctcctc gataaaactt
    13921 atcaagagcc ttttttaaat ctttagagct aaaccctcta ccagaatcac aaatctcata
    13981 agaaatacaa tttttttcat cctttacagt aatactaata ttaccacctg atggagtgta
    14041 ttgcagactg ttggatatga tattatcaaa aattcgtgtc aacctatcca cgtcgatttg
    14101 aattggggat tgtatattcc cctgcatttt caatataagt tcaacttcct tttgatgagc
    14161 ctgtaattca tagtcttgta ccttttgcct caaaaattca ggtaaattta taggaacaag
    14221 atttaattgt atatttgact tttctaagtc agacgtatat tgcatctttt ggacaagagc
    14281 agcacttttt tctgtatttt ctcttatcac tgtaagatac ctatgaagtt tttcattgtc
    14341 atctgtatta ttaccaataa gtgcatctgt atatcccaaa ataatagaaa gaggagattt
    14401 taaatcatgt gctagagctt ccaccatttc aacacgttcc tgctccatct tccactgtgc
    14461 agataaagag ccttttaatt cgtctttcat ttcagaaaat gcactacaaa gtttaccgag
    14521 ttcattatct gaataatagt cgatttcaaa atcaaggtcc ttttctttta tcttcttgga
    14581 cgcatcaatc aatagatgta atggttgatt gatattcttt acaaagcgct tagaaagcca
    14641 tctagtaaaa cccacgatat atagaaatgg agaaaacaga gccattataa taactgcaaa
    14701 tacaaatctc cctctaatat ttgcaaaggt gattttcact tggtagaata gaataacagc
    14761 tccctcaatc tttccattat cccctttgat tggaacagta tggatataac cacctttacg
    14821 aacagtttta ttgataaaat tattaaataa ttcttctttt gttttaaata gttttttggg
    14881 gttagtacca tataaaatat tgccattatt atccacaact tgatatagca tatcatcccc
    14941 acgaatcgtt ctttttaaac cttcttcatt cgattgtgat aacaaagtta tgtttttttc
    15001 attgatgtat ttttcaattc taggaacttg tcgctcataa tagtttggtg gatagatatc
    15061 cttatttaag gaatgaatat acaaatagag agcaaataca taagtaatca ctgtggcaac
    15121 tatactggct attataataa aaataaaagt atgtctaaat tgggtaataa gggattgatt
    15181 tttcatttaa taacctctct ctctttacat tttattccac ttatacccaa taccccaaac
    15241 tgtggaaata tattcttttt ctggtgtcac agcagaaaac ttagctcgta ttttttttac
    15301 tcgttcaaca acagttgagg aatcaccttc agaatcacac ccccatactt tttcatagat
    15361 ttgttctctt gagaaaactt gtcctgcatg gagtgctagt aattcaacaa tatcatactc
    15421 tatttttgtt aaatcaatat tcttaccatc aattttaatt gcacgttcct tcatatataa
    15481 gcataacttc ccaaaataca aagtagatct cttgttctct tcattgatat actgtgaacg
    15541 cttttcccta cgcagattag cttcaatcct tgccataagt tcacgcagtc caaatggctt
    15601 tgttacataa tcatcccctc caaaggtcaa ccctttgatt ttgtcggttt ctgattgctt
    15661 tgcacttaaa aatataattg ggcataaaac atcatctcta attgcctgac atacctcaaa
    15721 tccattcatt ttaggcatca taatatccag tataataagg tcaggctcct gttttgataa
    15781 ctctatacct gcctgaccat cataagctat caacacttca tgtccttttg ccttcaattc
    15841 atcctcaagt agcattacta ggtccatttc atcatcaata atcaatattt tactcaatat
    15901 aacaccacct ttctcttatg ggaaggtcag tattaaaaca atattcgtct actaataact
    15961 tttccttccc tcccacattt taaaccaaat aacgcctcct atcaagaata taatcaaacc
    16021 tattgctact actataagct ctatactagc tgtttgcatc actttagagt aaaatgcctc
    16081 agttgaaata gttccagctt ctttaataaa ataaattcct ggaagcatac ctagttttac
    16141 tggccatgtc catggaacaa atgcccatac tttttcacca acgctagtta atccaagtat
    16201 tgctgccatc aaaattccac cgatactaat accaatagat gcacccatac cccatgcaaa
    16261 actcacccaa agatgaatag ctaatagtgg aagtgtccca atcaccacaa gtcctgctgc
    16321 tgacaggaat aaccatattg ttgctccaga tggcactgca atactcattc ctatgcaaag
    16381 tatcaacgta gcaataaatg tgcatatagt taggcaaaac agaagaaaaa gaaattttcc
    16441 cagatataat ctaacacgag aaatacctac acataaaaaa ccattaaaat ttccagcaag
    16501 ctcttcctcc tgcactataa atcctgctaa aatacctact ccaataggaa tgatgaatac
    16561 agtccatact gtaaaaaatc cttcaaaagc aaattcttgt gtacttccag atttagttgt
    16621 gagataaaaa accacacata atgaaaaaat aataggcata aaaagagtaa gccactggac
    16681 agctgtcctc tttgctcgaa gccattctga tgataatagc tttatcattt gtcatccctc
    16741 ctattgaacc aagctgcagt aagtgctgtt aatactataa acacaaccaa agaaacaaca
    16801 atcccaatag gaatcactga agtattcaaa agtgggtctc cagcttctaa tatagtaccg
    16861 ttaggatgta tgttgatgat tgggcatacc attctaactg cccaactcca aggtactgca
    16921 atccaaaatg gttttgtcgc agcaaggaca ccaaatatca tacctaaagc tccaactccc
    16981 atacttagga acataccttt ccatgtagct agccatagct gaagtggaag tatagcaaga
    17041 gaaacaaccc agcatacaat acttgcccct ataatctgtc caaaaggaac tggaccagct
    17101 ttcaatatta aaccagttat aattgttacg actattaaaa ctagcgttga aataaaactg
    17161 tagactgcca tagctataac tttattaatc caaagtgtca taggagacac attgtgtgta
    17221 cgtaaagcac gataatttcc agcttttttt tcctggaaag ctaccaaagt agcaaacaat
    17281 gcaaatccaa taggcagaaa taaaaatgac caccagttaa aaattaatgc taaaagactt
    17341 tgccacgccc aagaagttgt ttgagataat ggattttgca tgagtgttga ttgcattata
    17401 aatgcatatg ctgcaaaaga cactgggaaa aaaacaataa gtttacccat aaatgttctt
    17461 ttatacttta aaagttctga ttgtaaagca ttcatctgtt ttgtcctcct ttaagaataa
    17521 catctgtaaa aaattcttct agattttcat tagggtcagg cattccttga aataatagtc
    17581 gtcctccatt tataattcct atattatcta caacttgagc aacctctgat agaatatggc
    17641 tagaaagaat tacagttata cctttttttg gaaaagaagc aatcaattca cgcaattctt
    17701 gaatgccaaa ggggtcaaga ccattggttg gttcatctag tattaacaac tctgggtcac
    17761 tcaaaagtgc aatagctatt cctaaccttt gtttcatacc catagaaaat tgtgaagctc
    17821 tctttttacc tgtatttttc aaatttacta tttccaacac ttcataaatt ttatctttag
    17881 gtattcccaa tatttttgta tgtactaaca aattttcctc tgctgtaagg tttccataca
    17941 atgcagggga ttctattaaa gaacctattt ttgcaagaga ttctcgtttc caaggctttc
    18001 tatcaaaaat aatacgacca tttgttgggc gaagaagacc aacaagcatt ttaagtgttg
    18061 tggactttcc tgctccattt ggtcctaaaa gtccatatat actatttcgt tcaatttgta
    18121 gagaaacatt gttaactgcc atttgttctc catatctttt tgataagttt tttgtttcaa
    18181 gaattaaatc tttcatatta atttctccct tcttgtaata ccttaaatct gttactaaga
    18241 gaatactaac atgaaaaaat aacaattcta taacacctac taattttaat aaaatttaaa
    18301 ataaaaaata gagttaccaa ttttattttg gcaactctat ttaagtttat gttttacagt
    18361 tatattagat ttaagcttag tttcattatc acactaactt caaatttact ctataatcct
    18421 taaatcaaac actaatttat taatcaaaca ctaatctatt aatttatatt tctttagaca
    18481 tctaatatag tttgctacta aataataatt atttatatca tatttatcta atcctttaaa
    18541 agaagttatt tcatcattaa gctcatcact accttctaca aaaacataac ctgtttcaaa
    18601 ttccatacct acaataatct tacagccact ataaatttcc tcttgagtat cgtacaattt
    18661 ttttgcattt ccttcatttg gaattgtaaa tacaataggt tttaattctt tatcactgga
    18721 aatcttttgt gaaaaaggct tgtttaactt aatattattg ataactacat gtttaaattc
    18781 ctctgaagtt tcttctacta ttacatattt tcgctttaaa aactctatta tttctaaaac
    18841 agacttagaa tttactttta aaatactttt atatttttca tataaagatt tccactgttc
    18901 aattttttcc tgtggtactt cttggatatg tgtacttatt ttttcttgag atattttttt
    18961 caattaaact ctcctttcaa tacttaaata tccttataaa tctcacatga ctaaagttac
    19021 aaggttccta cttaatagaa ttttgcatac ttaaatatgt cttattaatt ctctacagga
    19081 tatctccgta gttcctacga ttcttacata agttacttat tttaaattgt tattagttag
    19141 attataaaaa ttatttattt tatcactaat ataatactct taaaaacttt acaaatcaat
    19201 ttaaatttgc taccttttat ttataacaac ttatagtaca tttctcctat gcttttcaat
    19261 atattacttt taaaaggagg ttttcattat gattttaaat agagctgagc ttttccgagg
    19321 atattcttta aatagattta acgagatatg caatatatta tcagtaaata aaattaaata
    19381 taagtataaa gtgaaaaata atgcttactc taaaaaacct ttttttaatg aaataacact
    19441 tggcaattta ggccaaaagg aagatttctc ttatgagtac ttaatttatg ttcataaaga
    19501 cgattatgaa caggcagagt ctctaataaa ttctaagttg cgatagatta tctataaaac
    19561 atgacattct gttgtcatgt tttatatgtt attatacttt caaatatgct actatatttt
    19621 caaaattgtg tgctttaaaa ctagcttttt ttagtaattt aaagcttaaa actaaggaga
    19681 ctcgttatat gaaaataaat ataaaatcaa agttaagtga atttattaaa cagaacaatc
    19741 tttttgatga cagcagaata atatcctatc ttcctaatat aacaatagaa acagacaaaa
    19801 taaaaactat aatgattaat gaggttgtcc caactaacac caatgacgat ttttatagtg
    19861 tagataaaga tgctgattat ttaaaaacta ctattccttt atttgacagt gcaggaataa
    19921 aagtatctaa cataaatgat atcctagata tgggtatcta tataactaat gcagtaaaac
    19981 ttcctaaatc agaatataca ataactcgtg atacaattaa attacatatg cctattttag
    20041 aagaagaaat taaattattt aaaaacctag aggtagtaat gttaatggga gatgttgcaa
    20101 aaaaatcttt taatatgatt actaaaaagc atataaaaaa gaatgtcatc ccttccattt
    20161 caacatataa acttcgtaat aatgaacttt attatgataa tttaagagta tttccatcat
    20221 atattatgac tggtggaaac atactgattg aaaaatcaaa atttgaaatg gcatcagaag
    20281 atataaaaaa aatgttcgag attataaacg gttgatatta caaaataata aaaactaata
    20341 taaaataaaa agtgcctcaa aaacttctat ttatgaggca cttttatttt atattagtgt
    20401 aactatatac tattaacata atttattatt tatattgttc cttttaatct ctattccatt
    20461 tagtctaggt aacattactc tttacaagat tactttatat ttcttttata tctctaacgt
    20521 ttttatttaa taaaacaatt attaatacaa gtcaaatata tctatttatc taaaaatata
    20581 tcttttacag taatatctgg atggtgatac ttcttcctat tcgttaaaaa agattttttt
    20641 acattaatag cctcttttgg acaccagtga aaacaagcta ggcatctttc acactcatta
    20701 ccccactttg gctttttatc aactatactt atattctcta caggacaaac ccttttacat
    20761 gtcccacatc tattacattt attttctgca aaaaatttag tagttgcaat ttgcatattc
    20821 ttacctacta aacgaagtac aacactatct atatgtgact ttaaattgtt ttttccttct
    20881 atatttccaa tctccaagtt attgatacat tttgcaatct cagctaccct attagtctga
    20941 tttttcaatc tttcttcttc aacatctgga ggtgttatca aagcatttcc aggcatattg
    21001 attaaaaaac cagcagataa ttttttcccc ctttttttaa gtaacttatc tatagtaaat
    21061 aaactatgtc cagctacccc attacaagtg gcaatggcat atatatgagt attaatatta
    21121 aaatttaatt tctctacaaa agactttact atatcaggaa catcagcaaa ataaacagga
    21181 aaaacaatta ccaatatatc ttcatttatg tctatattat tttcttttct atgtatagct
    21241 aagggtataa cttctttact tccatttaac gtttctttta aaatatttga aactactagc
    21301 gaatttcctg ttccagaaaa ataataaatc ttactattca ttcttatccc tctttctata
    21361 tttctacatc atcaatagtt aaaaagtctc ttaaaatagg atttatattt actttccaat
    21421 tctatccaaa atatcagtaa acaaaggcaa ttcttcatca gagatagatt taaatgtctc
    21481 tgttgcatat atacttgcaa tttcatttaa tggttcttct aattctctac ctaaatctgt
    21541 taggaatatt tgaaacttac gtctatcttt aacatctact ttacggacaa ttaatccctt
    21601 atttttcata acatctaaga ttcttgtcgt attagattta tccttacatg atttttcaca
    21661 taaatctatc tgagaaattc catcttgctc ccatagaaac cttaataacg cataatggtc
    21721 agttgttata tcatatccat tttgtttaaa catctttttt aaatcatctt ttacttttaa
    21781 cgaagtactt actaacttag ttgtagcttc atatagtttc tcaaaatctt tcattttacc
    21841 tccatttaaa taatcccttg ctcctcccat ctatcaatta ttcccttata ttgctcaata
    21901 tctttaaaag ccattggctt taaaacagca tttatagcaa taaaattata gatttgagaa
    21961 agcacaggtg gatttttctt ttcctctatg caataaataa attttttaga taccttttgt
    22021 atctttgatt ctactttttc agttatagga ttttgattaa atccagtttt tatagataaa
    22081 ctaccacaaa cataacctcc cgaatctaag accatggatt ttataaaaga aattgctttt
    22141 gaaccacgtg cattttcata tgtagtaact gctatacatg gcttacgata caataatctt
    22201 tcaagcatca tatgtgctct atctgtaaac accttgaaaa gtccagttac actacttcca
    22261 taagttgggc taccaaatat aacaccatca ctcttagtaa ttatatcatg aatatactct
    22321 actttatcat tttgattgat acacttgccc attttataac aatttaaaca accaatacaa
    22381 tatgatggat tgacctcact taaatcaaaa taattaattt ttacatctgg tattaatctt
    22441 tctatatctt tatacaagta agtcaaaact tttgatgtag ctccattttt tctagggctt
    22501 ccatttataa ctgtaattat catttctata tctccttttt agttgttata tcaacaataa
    22561 gtatatcaac tatattttta gctgtcaata taaaccattt atttaaagct atacttacac
    22621 agcttaaata aaagttttat ttaactacat gtgcttattt aatcaactat aaatgaatta
    22681 tttttaataa ttgacaaact aaattaatga taatatactt tatgtattac aaatgtaagt
    22741 agaaggaggt atagtatgaa aaagttacca catatttcag aagcagaata tcaagttatg
    22801 aaaattattt ggaaatatgc tcctatcagc actactgaag taattgaaaa gttggtagaa
    22861 acaagtacat ggagtcctaa aacaattcaa acaatgttat tgagattagt taaaaaagga
    22921 gctttaacat atgaaaaaaa tagcagagta ttcgtttata ctccacttgt taaagaagaa
    22981 gaatacgtag ctacagaaag tagttctttt ttaaatcgtt tttataatgg tactcttaat
    23041 tctatggtac taaacttttt agaaaatgat aaactatctg aagatgatat tgaggaactg
    23101 agagaaatct taaataaaag gacgactaag ggggataaat aaatatgtat gccccattct
    23161 ttatacgatt tttactcagt acattaacct tatcaatatt aattgttgta attttacttg
    23221 ccaaaaaagt atttaaaaaa catatttctg caaagtacca atacaatatt ggatttatat
    23281 tgcttataat gttaataatt ccatttgtcc ctcttaaata tattaatctt ggaaatgtat
    23341 ttaactattt atatactttt aatcaacttg gaagcagtac taataggact tctatgataa
    23401 aaaatagttc aaattctgta attaataact caaatgcatt aaacgatttt tcagtatccc
    23461 taaatcaagt taattttgat agtttaaata ttttattact aatatcatgg ataattggaa
    23521 ttttaatcat gatagctatt actattcatt gcaacattaa aataaaaaat atgaaaaaat
    23581 caatacaaat tttaaaagat gaggagatag tatttatatt tgaaaagtgt aaaaaagata
    23641 ttggtataaa taaggatttt atactatgca aatcacctat tataaataca cctattacat
    23701 ttggatactt taaaccacac attatattac cagataagtc tatatctaaa ttatctttaa
    23761 aagatataaa atatatctta cttcatgaat tacaacattt taaaaataaa gatatactga
    23821 taaattatat aatgtgcttt cttcaaatat tatattggtt taatcctctt gtatggtatg
    23881 catttaaaga aatgcgtata gaccgtgaaa ttgcatgtga tatttcagtt ttaaaaaagc
    23941 ttaataaaga tagtcatata gaatatggac aaactctaat aaactttgtg gataatattt
    24001 caagaccatc aaatcttaca cttacatcag atattggtgg ttcaaaaaaa caaataaaaa
    24061 agcgactatt gaagataatg gattttcata atgaatcaaa tacgttaaaa ttcaaaagca
    24121 ttttaatttt tacattgata gctttaattg tatttggaaa tattcccaaa ctatctatta
    24181 tggcagatat caacgaaaaa tatagaccta caaatgaaca aactgtgtac gaggacttaa
    24241 gtcagtattt taaaggtttt gatggaagtt ttgttatgta tgatttaaaa tccaagcaat
    24301 atcatattta caataaagaa aaaagtaaaa caagaatttc tccaaattct acttataaga
    24361 tttatagtgc cttattggga ttggaaaatg ggataataac aagaaataat tcacatatcc
    24421 cttgggatgg aaagcatcat tctaatagtt cttggaatga agaccaagac ttattttctg
    24481 ctatggaaaa ctccgtaaac tggtactttc agactctaga taaaaaaaca gggctaaaaa
    24541 acatacaaac ttatcttagg caaattggat atggcaatta tgatgtttca ggtggtatct
    24601 caaacttttg gctagaatct tcattaaaaa tatcacctat tgaacaggta gaattacttg
    24661 aaagttttta tacaaataag tttaaatttg aagataaaaa tatagaaaca gtaaaagatt
    24721 cattgttaat atcaaaaaag gatggggtat cactttttgg taaaacaggc actgccaata
    24781 taaatggaaa agatgtaaat ggttggttta taggttatgt ggaaaaaaat gaaaatactt
    24841 atttctttgc tacaaatatt caaagcaaag attattctaa tggtagttca gcatctaaga
    24901 taacattatc catattaaat gacaaaaaaa tctataatcc ttaatatatt tactgtacat
    24961 tctaaaaaat tcacataatt ttcatattaa ttatcaatta aattcatata caaggcttat
    25021 tatataaaca tagaagatag taaaactagc ttaaaaacat aatataatac cctatacaaa
    25081 tagtggcaac tctccctcac atcctcaatt gttgccacta tttaattttt tgtatgaagt
    25141 taaatatatt ttcatcattt caacaatttg ggtgcaatac aatctctatt ttgaaacaac
    25201 ctctttaatt gttttagagt ttatgaatta tttctcatac tttaaaaaga ttgaattact
    25261 cttgataatc ttcttttgta gtcattacat atcacctact actcaacaca tattctttga
    25321 cctattttta catccttgtt tatatccaaa atatttacct tcttaccatc tggaaacaat
    25381 accattgtca aagtagactt tcctaattca tgtattctct ttaaatcaac attaacaaga
    25441 acgtctccct gtttaaccat atctccttgt ttaacagcaa catcaaagcc atctccattt
    25501 aatgcaactg tatcaattcc aacatgaatt aatacttctc cattagttcc tttaatccca
    25561 aatgcatgtc cacttgaaac cactgtttct aatttacctg aaataggaga aacaattttt
    25621 ccatctataa tatcaatagc aaaaccatca cctaacattt ttttagaaaa tgtttcatct
    25681 ggtacatttt ctaatgcaac aattttcccc ttagctggag acacaaagtt ttcattattt
    25741 tcattaaaca ctaattcttc gtcttcatct tcatttatag cttctttagg cactccaaat
    25801 aaacatgtta caataaagca gaatgcaacc acaaatattg atactaataa gtatgtgatt
    25861 aaattatatc catcatagat atacattaaa taagaaggta aaactgctaa tccatatgaa
    25921 ttagattgaa tatgtaaaat tgataatatt gctgctccta aacctgaaga tgcacacata
    25981 ataatcaaag gctttaaatt atatcgaagc tgaatcccaa ataaaacagg ctcacttata
    26041 ccaaaaagtt gagatagcat tgctccaacc gcagtttgtt taacttgatt atttttagat
    26101 tttttcataa atgctagaca tgttccaata ttcgcaaatc catacattgc acaaagagta
    26161 ataagggcat taaatcctgt atttgctaat agtgatgttt caatcattac ataagtatga
    26221 tgtaagccag taataacagc taatggatat gtaaatccaa taataaatcc tccaattcct
    26281 aaaggcaaat caatcaataa gctgatgata tcaaccattt ttaattctat tgtatgcatt
    26341 atagggccta cacctaacaa aataactaac attgttatta gcataacaaa aaatggtgtg
    26401 aaaataaggt ctaatacatt aggcatatgt ttacggaacc atttttcaag attagctcca
    26461 ataaatgctg taacaatagc tgttaaaaca cttccttgac aacctacaat aggaataaat
    26521 ccaaatgcca taattgcttc aatttcattt gatgggtcag caacagaata tgcattagga
    26581 agtattgggg aaactaacat aagtccaata acaagtccaa taacaggtgt tcctccaaat
    26641 actttaaatg ctgaccatac aataattgct ggtaaaaaag caaatgctgt ttctgtaaga
    26701 accgaaacaa gtgtctgaat atacaatgga atatttgcag aagacattcc aaataatcca
    26761 agtacattat cattaaacaa acaacccttt aatcccaaga ataatcctgt tgctgcaata
    26821 actggaataa taggaacaaa tatatctgct agagttctca taactttctt aaatccagtt
    26881 tcattatttt taactaattc atcttgttct ttttttgata aagtttttaa tcccattttt
    26941 tcaacttctg cataaacttt attaactatt cctgttccta aaataatttg atactgtcca
    27001 gaagtaaaga aaaccccttt aacctcatca actttttcaa ctttcttatc attaataatt
    27061 tctctatctt taaccatcac tcttaatctt gtggcacaat gtgtaataga aataatatta
    27121 tcttcaccaa taattttaat aagctcattt gctattttat tatatttatt catttttcta
    27181 cctcactttc aataatactg catgaaatgg tttcaaacgc actgttttat ttaatagatt
    27241 tatatcatct aaattttgga atacaatatc atatttttct tctactctta tttctacagg
    27301 tttatcagaa aaattataat aacaatcaat aactaccaaa tctaattgcc ttctatacgc
    27361 aataatatca tcactttcta tatcaatagg ttctattgaa ccataaatca aacaatcaga
    27421 atactcacta tcttgtctca actttatcat ctttttataa aattctaaaa tactctctga
    27481 attgttaatc tgactctgtg cattaatatc tttataattt tcaataacct ttaaccatgg
    27541 tgttgtatct gaaaaacctg cattttcttt atctgcccat ggaaatggcg ttcttgcatt
    27601 atctcggctt cttaaattca caaattctaa cgcttcttct tctgagtaac tttcatctat
    27661 tgaacgatag tattggtcaa tgctagataa atcattaaaa tcatttatgg tatctctttt
    27721 aaaatttgtc attcctaatt cctgtccctg ataaataaat ggggttcctc ttaaaaagaa
    27781 atacattgct cccaacattt ttactgcatc atcattcctt tcgtattgca aaagatactt
    27841 tgttgtagct cttggttggt catgattttc aataaaatta gctgcccatc catgttcctg
    27901 aacatacatt tgactatcta aaatgcattt tcttaaatct ggaattgtcc atgaaattct
    27961 tttaaaccac tcactaccag aagcaacatc taagtcagca tgtttaaagt caaagaccat
    28021 tgaaaagaaa ccatcttctc caataaaatc acctagctgg tcatagctaa tacctgctgc
    28081 ttcagcaaca gtcatacaat catatttttt aaacgtattt tcttttaatt cataaagcat
    28141 tttttctatt ccaggctgat ttctaactgt ttttgtacat ttaacaagac catcaacacc
    28201 atcactttct aaactttgaa aacttaaatt ctttttgatg aatgtaatag catcaactct
    28261 aaatcctgcc actcccattt gagaccaggt attaatcatt tggtaaagtt cacttcttaa
    28321 tattggattt tcccaattaa ggtcaggttg ttttttacca aaagaatgaa aataatattc
    28381 gtctctattc tctacctttt cccaaacact tccaccaaaa acagacctcc agttatttgg
    28441 taaatcgttt ccttttttaa aaatataata atcatgataa ggactttctg gattttttaa
    28501 tgcttcttga aaccaaggat gttcatcact tgtatgatta agaactaaat ctaatataat
    28561 tttgattcct ctatctttac tttctttaat taactctttt aaatcttcca tacttccaaa
    28621 ttcacttgca atatccaaat aatcactaat atcataccca ttatcgtcca taggtgattt
    28681 aaaaattgga cataaccaca atgatgtaat acctaaattt tttaaataat ctaaacgaga
    28741 aataattccc tgtaaatctc caattccatc attattacta tcttgaaagc ttcttggata
    28801 aatttgataa acgatttctt tttgccacca tttcttttcc ataaattact cctttttata
    28861 tgatatttct tctttttaat tctttataaa ttccattatc gaatatgtcc tcacatatag
    28921 atgtcgctat atctttaagt tgttgatgac tatttttcat tgcaattgta acgtctgatg
    28981 cttgaaacat gacaacatcg ttttgaccat caccaaaaca tatggtttct ttttgagtta
    29041 ctcctaatct ttcttgtaat ctcttaattg ctttgccctt atgaaaatct ttttgaataa
    29101 tttcataata ctgactagaa atatctctct gtgctaattc cattttatct tgtagtatat
    29161 ttttcacttc gtcaaatacc ttttcatttg accataaaca aattttatgt atatcttgtg
    29221 acttatactc ttcaatgtta ttttcatatg ttattttttc ttggatatgt tgtttattta
    29281 tacaagaatt agttcctttc aactgattca ttgtttcaaa tatttctttg gctttttgat
    29341 tcatgaaaac tttttcctga ctctcaatcg aaaaagcaac ttctctcttt tttaataaac
    29401 aaacaacttc ctttatcaat ctttgattaa aagattgatt ataaagtaat tcaccatgat
    29461 attgaatgta attacctcct cctgcaatat atccatcaac tcctaatgat aaaacatcat
    29521 cttgtattgt acccatactt cttccagtac aaataacaac actgcaatga ttcttttgac
    29581 ataaacgtat agcatgcttt gcagattctg ggattccata tacctcatct ctaagcgtac
    29641 catctatatc aagtattaat aatttgtaca atttcatctt cctttcttac tatcaaaatc
    29701 ataatagcat tcaaaaaaaa agatatcaat attttgatat cttttaaaaa atatgttcat
    29761 ttttagcaca aattttcatt ttcggggaat atgttcactt tctaaataca tcttgtattt
    29821 aatataaagc agttcaatta aaataaaata aggtgttgtt acatcgtagt ccagttgttt
    29881 atctacagaa atagatatag attgaatata caggttttca tcacataatc ttgctaaagg
    29941 attacttatc atttttgtaa tagaaaccat ataaattcct ttcattctca tttctttagc
    30001 aaaatcaata attgtagatg catctccatt caatgaaata aagaaaatca tatcatctgt
    30061 ttttataaaa tgattaagtg cctgaatcat atcataacca taaacatcat atataatttt
    30121 ccctgttggt aagaaaatcc ttttcatctc tttagcaact ctttcctgag aataaccttc
    30181 tccaaagacg attatacgat ttgccttttt cattctttca aacataatcg aacaatcctt
    30241 cttctctata tcatctatca ccttattata acaatccatt aattggtgtt tactttgatt
    30301 tttatattct acttctgttt catttaagcg tataataact cttaattcac tatatccagg
    30361 taattgtagt ttttgagcaa aacgagaaag tgcacttgta gatatatggt atttgtctgc
    30421 aaattcatct atagaaagtt taatacaatc ttctttatga ttaagaatac actcacagat
    30481 atacttatca tttgctgtaa atgaattata atatttattg aaaagtcctt caattttcat
    30541 tcttttctcc ttctatactc tatgtaattc atatgaagca aatctaatag agcataaaat
    30601 gctcctacga cttcataaga tatagcttgt aatgtatcaa gtttttgcgt ttcaacatac
    30661 agattatact cacaaagtgt tgcaattgta ttattatcaa gcctagttaa agaaatcgta
    30721 tgtatatgat tgttgataaa ttttgcaatt tcaattcctg cttttgtttc tccagacaaa
    30781 gaaatgataa taaataaatc atttttttga aatgattctc tcataaattc aatttcacct
    30841 atatcaaata agtcaataac acatttacct actgaaagaa atattctctt tatttcttga
    30901 gctaaacttt tctgttcatt acctgtccca taaatataaa ttgtttctgc atcatatatt
    30961 attttacaaa tatcttgaaa tgatattttt tgtaattcat tcattaatag tcgataattc
    31021 tcgcaaacat catgaaaatt attatcatta ttaacaaatt ctagttgatt atttttaagt
    31081 aaatacttta aatcagagaa tgaatctaat ccaatttttc tacaaagcct taataaagta
    31141 gctctagaaa cattacattc atttgcaatt ttttcacacg aaaaattacg gtattctctt
    31201 tgacaaatca atatttgttt aataataatt ttatcatttt catttaaatt atcataattt
    31261 tggttaacta acgattctaa tctcattttt tccactccct aaatattatt ttaatataca
    31321 ttagttgctt tttctaggga agagttttaa ataataagga taaaataatc atttgatttt
    31381 atttttatta aaataaaaat aacctgctaa aagcaggcac caaaaagtac ttatcctttc
    31441 gaacatgtac tttagatatt gctttcttcg gaagcttcat ctcttcatac tctaaaatat
    31501 agttttttct aactcaaaag tattttccat ggtagaaatt actttaattc atctgccatt
    31561 tactttttat cttatttaat aattactatt acttgaatat ttacttcttt aagtaaattt
    31621 catagttaac aaagctaaaa ctattattat agatgttaaa atacaaaatt gtaagtattg
    31681 ttataaatat gcttacagct acaaatatac atgatatatt aaaaagaagc tgtaaattat
    31741 tatcatacat acaagcaact atgcctctat taatccatgt taatttttat tatttaatac
    31801 aaggagttcc atgagacata catatccatt catattttaa attatccaat tctcttatag
    31861 atttcaccat ctttttataa tcccaattcc atggattagg ataaggaact atatttccat
    31921 tcttattttt gactaaatca cctgcaaata atacagcttc aaaaatcatg cagacatgcc
    31981 ctggagtatg accaggagta tggataattt ttatattccc aatctgcata ttctctccat
    32041 aaggttcaac atccttaatg agccttctgg aaattgcctt tccaataaac ttcttaaacc
    32101 caggtctatc cttctctcct ataataaaag gtatatcctc tacatgagcc catacttttg
    32161 ctcctgtttt ttctttcaat aatttaatat tagatatatg gtcaacatca tgatgagtta
    32221 ataaaatatg cttaatatct gtaagtttaa tgccaagtgt ttttaattct ttaattatat
    32281 ttttccccat aaaaggaaga cctgtgtcta taagaataat ttctttatca aacacaataa
    32341 aacaatttga gccacgagta ctatctaaca ttaatacatt ttcttttatc ttcataaata
    32401 aattcccctt atttggtatt ttacctatat aattaattaa taaagttata aatagttata
    32461 atacttgata ttaattttat tttttattaa agtatactat aggtatacaa gtattatatt
    32521 tacataagta taccatcagt atactttttt atcaatagaa aggaataatt tatatgtcag
    32581 aattatcaaa aagagataaa gaaaaaatac aaagagaaaa tgagattatt gataaagcag
    32641 aaaaattatt ttgtcttaat ggatttgata atacaactat gaatgaatta gcaaaagaag
    32701 ttgaatatac taaacgcacc atatacaagt acttttcatg taaagaagat ttattctttg
    32761 ctgttgtctt aagaggatat aaaagacttt gggataatgt taaaatagaa agtgcaaaag
    32821 gcaaaacagg gtttgaaaaa ataaaacttt catactttgc ttttcataaa ttttactctg
    32881 tagaaccttc tctattatgc cttatgggaa tgattggtat tgtaaactct gaaaaatcgg
    32941 atactgatat gctgtttaaa gaaaagtttt tttcttttaa taaatttatg tttgatgaaa
    33001 tacaaggaat gtttgaaatt ggaaaaaatg atagaagtat taggcatgat attgaaatac
    33061 ctactcttat gtattcatca atatttacac ttacaggatt ttttaattta ctatctgtaa
    33121 caggaaaatc atacctgaac aactttaata tagatgagga aaaatttatt gaaacaactc
    33181 ttgctctttt aatagattca attaaagcat aaacctaagt ttataaatta aagtttatat
    33241 acttaaatga taattttttc tatctctaat ataaaaaata ttaaacataa aactaataac
    33301 ttagttaatg gaataactaa attactgaaa ggattaaaag taaatggcta aaatacttct
    33361 tttagaagat gatgatacaa ttacatttgg aataaaaact tcccttgaaa agaaaaatca
    33421 cactgtatgt tgttataata cgattgctga agctaaaaaa aattttgata aaactattga
    33481 tttaattctg cttgatttaa atctacctga tggtattgga tacgagtttt gccaatatgt
    33541 taaaagtata gaagatactc cagtgatatt tttgacagtc cgtgatgatg aaaaagacat
    33601 tatacgtgga ttagatatgg gtgcagatga ctatgtggtt aaaccgtttt tgctctcagt
    33661 acttcagtcc agaattaatg cagttcttag aagaactcat tcaaatacta acaatactat
    33721 tacatgtgaa aatataagct tgaataaggt tgaagtcaaa gcatatttag ataatgttga
    33781 agtacctttg acagcaggag aatataaact tcttctaagt cttatggaaa ataaaaatag
    33841 agctatgact cgtacaaagt tactagaaat gctctgggat gtagatggta atttcgtaaa
    33901 tgataacact cttactgtta caataaaacg tcttagagaa aaattaaaaa atccaccttg
    33961 tataaaaacg ttaagaggca taggatatag aatggaggac agctagtgaa aactccaaga
    34021 aatatcaaca gtacagttgt ttttataata ttctgcttaa ttgtaacttt tttattgagt
    34081 gtaattctaa caactataac ttcttttaat gaatacaatg tactgtctaa ggctcttggt
    34141 gcatcatatg agtacaatcc taagtctaca gaaagtatac taaaatcact gaaaaattct
    34201 agtgataagg attttaaaac aggaaatgaa attttatcaa aacatggata ttctaaaagt
    34261 acattttgga gtaaaaactt tttttatttt ctagtgatat cgtttggtat aatttcaaca
    34321 atggctacag ttatatatat aatcaaacat agaacaaata agcaaaaact gagtagaata
    34381 aacaatctta ctcaatactt agaagaagtg aatttaggaa aagagtctgt acttttacga
    34441 tgtgaagatg aattttctca ccttgaagat gagatatata aaacagttgg ggaacttcgt
    34501 tgttcaaaag aatttgcttt aaaagaaaga caaactcttt cagataatct tgcagatatt
    34561 gctcatcaac ttaagactcc aattacttct atgtcactta tggcacaatt actttcagaa
    34621 aattgtccag atgaagaaat agattatatt aacagactta caaatcaaat ctcaagactt
    34681 gagagactag tttcatcttt acttacatta tcaaaattgg atgcaagtac tttaatattt
    34741 gaaagcaaac ttatagatgt acattctctt cttacttatg caattgagcc tatagaaggt
    34801 tctttaagag aaaaacatca gacatttact atcgaggctt ctccttcggt agaatttgaa
    34861 atcgatatta attggacttt agaagctctt ttaaatattc ttaaaaattg tagtgagcat
    34921 atagaagatg gtggatatat agttgcttat tatagtcaaa atcctctata tgttgagatt
    34981 atcatagaag ataatggtaa ggggtttgct caggaagaac ttccatatat ttttaataga
    35041 ttttatcgtg gtaaaaatgc tagtaaggac agtattggta tagggcttgc tctatcaaag
    35101 tctattatag aacgtcaaaa tggtactcta catgcagaaa attgccataa tggtggtgca
    35161 agattcataa ttaaatttta cacataaaat gctggattat aggtaaaata cctataatcc
    35221 agctatatct atattctatg ttatctattg ttttagtaat atacttctat ttacatttaa
    35281 ggttttatta aacaaacaac ttaactatcc ctatacttgt aagtagtctt aaatcgaaac
    35341 ctttaaataa gttcaaatag attttgttta tattgtttca ttttttatag attctactat
    35401 attttcattc ttcaactgct tgtatcctgt catatatgat gctattacaa taaaaagtat
    35461 caatatagta aaacctatta ttgtagatat aggtgcaaat ggtaaatact ctataaaata
    35521 tatttcattt atacgtagaa aagcatacac tattcctatt tgcacaggaa tactaagtag
    35581 taatggtgta attcctaaga ataaaccttc caaaataagc atctttttta tgccttctgg
    35641 agataatcca acagaacgca acatagcaaa ctcttgtcgt ctacttctaa tatttcctga
    35701 cactgtagca agtacatttg ataatccaat catagccaat aaacctgtaa taaagcctac
    35761 tattaaattc atggtcttta ttccactagc cttttgtttt tcactttcta aaacatcctc
    35821 aatactataa tctcctgaac catagtcttt attataaatg gactctaatt tttctcttac
    35881 tctaggaatg tcctcacgat tatttacttt aaatactcct atcagcctat ttgaacgtaa
    35941 cctacttact tcatcataat ttttagatat ttcctgtacc atagacattg gaactatttg
    36001 cattaatgta tactttgaac tagtatctac tattttagga agtgtgcttg taatagctcc
    36061 cactttcatg ttgtaagtat aatccccttt atcttcgtca tatacttttt catttaattt
    36121 aatgttatct cctattgaaa gatttaaata atctatatat actttatttc tacgtgtact
    36181 acgattaata tcttcctcct tattatatac aattgaggtt ggattttttg tattataaaa
    36241 taactttggg tctacagaaa tttgcctaca atattctgta aaactcttat catcaagccc
    36301 aattatattt gaaattatac gaaacttgcc atcacgttct ataggagagt attttttaga
    36361 tgaaactata cttttaaatc ctccattttt cgcaagttct tttgatgcat ctttttcagt
    36421 cagccaagtg gaagcagaca ttttttgata atatagtgaa ctttttacac cttctgtatt
    36481 atctaatttt tcaatgaaag cagactctat aggttcacta ttttccaaat aaactgctat
    36541 atctctttgg tccttataat tatcacttcc ataaacagct tttgctcctt cttgatttgc
    36601 aattatatgc aaaaaacatg taagtaataa aaaagataat gttaaagata ttgttgcagt
    36661 acgataagat tttttacgag cacgtagtgc gtttgatgca agttctcctt ctattccaaa
    36721 tatctttcct aatgaatatt tatgcgactt tttgatttta acactatctc cttgtcgtat
    36781 tgcttctata ggagatatct ttgatacttt tcgtgctggt ataagtgcag aaatccagac
    36841 tgttactatt gtaagtaaaa ctgctggcag aaaagcagga ataccataag taaataccac
    36901 ttctggaaca tcagtatgat aattaactga attaacataa actataagcc tattacacaa
    36961 taaccatcct aaaaataaac ctattggtaa tggtataatt gtcagtaaaa agccttcaaa
    37021 cactacagac cttttaattt gttttggtga tgcaccaaca ctagataata tccctaactg
    37081 agtaagtcta gcatttgcag acagtgaaaa cgcgttgtaa atcaccatta caaacacggc
    37141 tactgtaaaa attgctattg taagatacat aataggagtt gaaaattttg atatactact
    37201 catagatgct ttctgttctg gtgaaaagac taacatcttt aaaagataat tactattgta
    37261 tttaagattg tattttccat attcattagt cttccatccc aatgatttag caagttttgg
    37321 aagttcctta tacgtgtctc ttatattttt aaatcgcaag tacacagtta tactatcatt
    37381 aggttttata cttgttttat caagatatcc aagagctgta tatgcaggta ttactgatga
    37441 agtagaaaca tctacctttc ctactattgt caatgtagcc tctgaaattt ttttaaaact
    37501 ttcccctttc gattttgggg ctatgggttc aagaggcttt ccatttaaga ttctatatcc
    37561 tattgggaga gtaattttat caccaatttt tacacttgga ttattctcaa aatattgttt
    37621 tgataatgca atttcattag cattatttgg tcgacgaccc tctattatag tatccttttc
    37681 tggcatagaa ttccaatact ctgatgtagc atttctataa attatataat ctcttttctc
    37741 atcattaatt ttgcctacat accattgtcc ttttaccatt acatcatcaa ctgaagaaaa
    37801 attttcaatt aatggtaagt cacttccata agtatagtca aacaattcac catgccagtt
    37861 accttctttc tcaattgaaa gtcttataaa atctgtccac atggtataca acagtccaca
    37921 caaagaagac atcatggttg tagtaagtaa tatagctaac attatagcta tactactacg
    37981 tttattgcgt cgtacaaagt ctaaggtgta ttcttgaaga atattcattt cttcaccacc
    38041 ttttcagaga caatcactcc atcttccata gttacaatac gatttgcctc tagtgctatt
    38101 ttctcatcat gagtaataag aagtattgtc tgattaaatc gtttatttga taagtttaac
    38161 aagtctacaa tttcttctga attttttcta tcaagattac cagttggctc atctgctaat
    38221 aaaatagctg gtctatagat taaacttcta gctatagata ccctttgttg ttgtccacct
    38281 gaaagttgac ttggaagatg gtttaatcta tcagataatc ccaatataga tacaatctca
    38341 gagaatctat cctcatccac ctttctttta tcaagtaaca tgggtaataa tatatttttt
    38401 ctaacatcaa gggtaggtat aagattataa aattgataaa taagacctac ttttctacgt
    38461 ctaaaaactg caagagcttc ttcctttaaa cttgatattt ctgtatcttc aacataaatc
    38521 tttccagatg taggtctatc tactccccca agcagatgta acaatgtaga tttgccagaa
    38581 ccagatgctc ctacaatgga tacaaaatca cctttttgaa ctgataaatt tacattatta
    38641 agggctgtca ctctggtttg atttgaacca tatatttttg tgagattttc acattttaat
    38701 atttccatat acaatcatct cctttatggt taaattttag caacctaaaa tgacattata
    38761 gtgacatgaa tacaaatata aattactatt aaaaatattg tttagttttt gcacattatt
    38821 taaaattata atataaaaca aaaactatgc aatatatttt atttatactt ttaatatatt
    38881 acatagtctt ta
//
LOCUS       DAMKMI010000021        37160 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMKMI010000021 DAMKMI010000000
VERSION     DAMKMI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.022"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(370..1734)
                     /gene="rlmD"
                     /locus_tag="PC429_001022"
     CDS             complement(370..1734)
                     /gene="rlmD"
                     /locus_tag="PC429_001022"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF3846539.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(1909..3669)
                     /gene="pyk"
                     /locus_tag="PC429_001023"
     CDS             complement(1909..3669)
                     /gene="pyk"
                     /locus_tag="PC429_001023"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF3846540.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(3721..4680)
                     /gene="pfkA"
                     /locus_tag="PC429_001024"
     CDS             complement(3721..4680)
                     /gene="pfkA"
                     /locus_tag="PC429_001024"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF3846541.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(4889..8458)
                     /locus_tag="PC429_001025"
     CDS             complement(4889..8458)
                     /locus_tag="PC429_001025"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF3846542.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(8615..9562)
                     /gene="whiA"
                     /locus_tag="PC429_001026"
     CDS             complement(8615..9562)
                     /gene="whiA"
                     /locus_tag="PC429_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF3846543.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(9579..10004)
                     /locus_tag="PC429_001027"
     CDS             complement(9579..10004)
                     /locus_tag="PC429_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF3846544.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(10073..11179)
                     /locus_tag="PC429_001028"
     CDS             complement(10073..11179)
                     /locus_tag="PC429_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF3846545.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(11191..12048)
                     /gene="rapZ"
                     /locus_tag="PC429_001029"
     CDS             complement(11191..12048)
                     /gene="rapZ"
                     /locus_tag="PC429_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF3846546.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(12079..12792)
                     /locus_tag="PC429_001030"
     CDS             complement(12079..12792)
                     /locus_tag="PC429_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF3846547.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(12796..13710)
                     /gene="murB"
                     /locus_tag="PC429_001031"
     CDS             complement(12796..13710)
                     /gene="murB"
                     /locus_tag="PC429_001031"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF3846548.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(13983..14753)
                     /locus_tag="PC429_001032"
     CDS             complement(13983..14753)
                     /locus_tag="PC429_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF3846549.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
     gene            15071..16552
                     /gene="cls"
                     /locus_tag="PC429_001033"
     CDS             15071..16552
                     /gene="cls"
                     /locus_tag="PC429_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF3846550.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            16870..17367
                     /locus_tag="PC429_001034"
     CDS             16870..17367
                     /locus_tag="PC429_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF3846551.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            17384..19270
                     /locus_tag="PC429_001035"
     CDS             17384..19270
                     /locus_tag="PC429_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF3846552.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            19293..21074
                     /locus_tag="PC429_001036"
     CDS             19293..21074
                     /locus_tag="PC429_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF3846553.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(21214..23010)
                     /locus_tag="PC429_001037"
     CDS             complement(21214..23010)
                     /locus_tag="PC429_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF3846554.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(23224..24153)
                     /locus_tag="PC429_001038"
     CDS             complement(23224..24153)
                     /locus_tag="PC429_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF3846555.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(24153..25970)
                     /gene="uvrC"
                     /locus_tag="PC429_001039"
     CDS             complement(24153..25970)
                     /gene="uvrC"
                     /locus_tag="PC429_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF3846556.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            complement(26229..29054)
                     /gene="uvrA"
                     /locus_tag="PC429_001040"
     CDS             complement(26229..29054)
                     /gene="uvrA"
                     /locus_tag="PC429_001040"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3846557.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            complement(29073..31043)
                     /gene="uvrB"
                     /locus_tag="PC429_001041"
     CDS             complement(29073..31043)
                     /gene="uvrB"
                     /locus_tag="PC429_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF3846558.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(31230..32492)
                     /locus_tag="PC429_001042"
     CDS             complement(31230..32492)
                     /locus_tag="PC429_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF3846559.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            complement(32555..33859)
                     /locus_tag="PC429_001043"
     CDS             complement(32555..33859)
                     /locus_tag="PC429_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3846560.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(33890..34750)
                     /locus_tag="PC429_001044"
     CDS             complement(33890..34750)
                     /locus_tag="PC429_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF3846561.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(34794..35663)
                     /locus_tag="PC429_001045"
     CDS             complement(34794..35663)
                     /locus_tag="PC429_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF3846562.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(35647..36717)
                     /locus_tag="PC429_001046"
     CDS             complement(35647..36717)
                     /locus_tag="PC429_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846563.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
BASE COUNT        11418 a         6683 c         4584 g        14475 t
ORIGIN      
        1 cgacacgttc ctctgattcc gtccagtatt gcttgctttc tgcatggacg gcggtactcg
       61 gtaaagcagt aacgacagtc gcaaaggcaa gtactcccgt acacaatcgt ttcaatatct
      121 ttttcataat tctaaatgct cctttcattt tgggtaaaaa aatagacgct caatattgaa
      181 cgtcttacat tgaaaaggga tactttttac aagtacccct caaatctaat atttaattgt
      241 ttgtgttccc acactctata tcaacaacta ctgaaacatt gatatagttt tttatctctt
      301 tatttctgat tttatcgccc tcgctcgtaa agactatatg cttaaaaaag tgcctaaaat
      361 taaggatttc tacatagttc gtctttgtag caacacacaa gtctctatat gtgtagaatg
      421 agggaacata tctacaggtt gtgcctcaac acatttaaat ccgtgaacat ttagataatc
      481 taaatccctt gctaaagtag aaggattaca agaaacatag acaattcttt ccggattcat
      541 tgaaacaata gtctctaata ccttttcgtc acatcccttt ctaggtgggt ctacaacgac
      601 tacattggca gtcttaccct ctctatacat cttaggtaca atttcctctg cctttccaac
      661 ataaaattca gcattatgta tattgtttaa tttagcattt atcttagcat cttttatggc
      721 atcatctata atttctattc cataaacctt tttagccttt tgtgccaaaa ataaagatat
      781 tgtacctatc ccacagtaaa tatcaaaaac agtatcagta tcatttaaat ttgcatattc
      841 aagagcctta tcatacaata ctttagtttg gacagggttt acttggaaaa atgataatgg
      901 tgaaatctca aacactaagt ctcctatata atcgtttatt tttccatctc catatataac
      961 tatattttct cttcctagaa tagcattcgt atctttttga tttacattta caaccaaagt
     1021 cttaaatcca ggtatatttt ccttaagcac tgaagctaat tctttaagat atggaagttt
     1081 gtttccattt gctactaata caaccattac ttctttagtt gtaaaaccta tttttgttac
     1141 taaatgccta atcaaccccg tgtgagtctt ttcatcatat atgcttacct tataggccct
     1201 tatatatgtt tttattattt taacaatttt atcatttatc tcatgttgta ttacgcattt
     1261 ttctgtcgat attacatcat gagtcttctt tttatagaag ccaataattg gaataccatt
     1321 attcttttgt attggaaatt gtgctttatt tctatatcta tatggattat ccattcctat
     1381 agtgtcatga acttctacat cctctaactt acctattctc gaaagtactt gtttaacttc
     1441 atttgttttc atttcaagtt gtttattata atctaagtct tgtatctgac accctccaca
     1501 atccctaaga ttgtcactac acactctttt caccctgaat ggagactttt ctattatctc
     1561 aacaatatct cccacagcat aatttttctt tactttatta atcctaactt taactttatc
     1621 atgtattaat ccgccctcta caaaaactgt aataccattg tactttccta ctcctactcc
     1681 gccttgacca atgtctacta tatctacaat atacatctcg tttttcgaaa gcataaatca
     1741 ccctccaatc aatctattat atttaaatct ttacaaactt ttttctttaa atttatatta
     1801 aaatattgct attaattaaa ctatattttt tactaagcta caatatttta ttttaccaaa
     1861 aaaaaaggag cttataaagc cccttagtaa ataaatttat tatttttctt ataaaactct
     1921 agttgaacct ctatatacca cgcctcttga tgcatccaca gttacaactt ctccatcttt
     1981 tactagattt gttatatcag ctgcagacac aataactggt atatctaaat ttatacctac
     2041 aatagctgta tgtgatgtca ttccaccatt ctcagttact acagctcctg ctttttctat
     2101 ataattgttc atttcagcat cagtagcagt agaaacaagt atgtcgcctt cattaaagtc
     2161 tacacaagca tttccattct ttattatacg aactttacct tctactgttt gagtacctac
     2221 acctatacct tgcacgattt cttcgcttat tacatgaacc tttattaaat tagtagttcc
     2281 acttactcct actggtactc cagcagttat aactactaat tccccattat ctatataatc
     2341 agcttgtcta gctgcctcaa ttgccttttc tataacttca tctgtatttc ctgcaacaga
     2401 agacttaact ggatatactc cccatgtaag agctaattta ttcatagtct tttcattatt
     2461 tgtagtagct attattggag attttggtct gaatttagat accattctag cagtatatcc
     2521 tgaagaagta gatgttatta tagctgatgc atttaaatct actgctgttg tacaagttgc
     2581 atggcttatt gcatctgtta cagtaacatt gttagtacca ttttccttta gtaatctatc
     2641 ataatctaaa gtctcttctg ttctttttgc tattgtagcc atcattttta ctgcctctac
     2701 aggatatttt ccagcagcag tttctccaga taacattatc gcatctgttc catcatatat
     2761 agcattggca acgtctgtaa cttctgctct tgttggtctt ggatttctta tcatagaatc
     2821 aagcatttga gtggcagtaa caactggctt tgctaattca ttacattttt ttatcatcat
     2881 tttttgaact attggcatct cttcagttgg tatctcaaca cctaagtcac ctctagccac
     2941 cattatacca tcagaaactt ttaatatttc atctaggttc tctacacctt cttggttttc
     3001 tattttagat attatttgta tatcagttgc attattgttc tctaatactt ctcttattgc
     3061 tagtacatca gatgcctttc ttacaaaaga agcagctata taatctattc cttgacttat
     3121 tccaaactct atatcactta tatctttagg agttattgct ggtaaattta tcttaacacc
     3181 aggtacattt actcctttat gatttttaac tattcctgaa ttttcaacaa cacaaactat
     3241 atcttctcca tttatttctt ttactcttaa gccaactaaa ccatcatcta ttagtataga
     3301 atctcctgat ttaacgtctt cagcaagacc tttatagctt actgtacaca tttctttagt
     3361 ccccataata tctttcatag ttatagtgaa cttttgccct tcttctaaaa atacctctgg
     3421 gtcttcaaag ttacctgttc taatttctgg acctttagta tctaataata tagcaactgg
     3481 cttatttaat ttttctctaa ctttctttgc catatcaatt ctttctttat gttcttcatg
     3541 cgaaccgtga gaaaagttaa atctacacac atttagacca ttttgcataa gttgagttaa
     3601 aacttcctca ctttgtgatg ctggacctag tgtacaaaca atttttgtct tctttacatt
     3661 atttaacata tttgctcctc ctgagatatt attccacaga tttattttta atactaaaac
     3721 ttatatagat aatatttttg ccatctcata agcttcttta tcaaatactt tcttttgagc
     3781 taaagcttca cttatttcta aatcaattat tttgttttct tttattccta caactctagc
     3841 tgattttcca tctaataata attctactgc tcttactcca agtctacttg ctagtattct
     3901 atcagataca gttgggcttc ctcctctttg gacatgcccc aatacagtga ctcttaaatc
     3961 tgctcctgac tcttttttaa gttccttttc taaatcacta gcacttccga ctccttctgc
     4021 taaaacgatt atactatgtc tcttacctct tttttgagta gtctttaatc ttagagcaac
     4081 ttcatctact gtaatttcta cttctggtac tattatagtt tccgctcctc ctgcaagacc
     4141 tgcatataaa gctaaatctc cacagtgtct tcccataact tctactatgt taactctttc
     4201 atgagatgat gatgtatctc ttatcttacc tatagcatct ataatagtat tcattgcagt
     4261 atcaaatcct atagtataat ctgtatatgc taaatcgtta tctatagttc ctggtatgcc
     4321 tatagctggg aatcctaagt cactaagttt ttgggctcct gcaaatgaac catctccacc
     4381 aattacaact aagcaatcaa ttttatactt tttaagaatt tttactgctt ttaatctacc
     4441 ttcttctgtt ttaaattctt cacatctgga agattttaat atagttcctc ccctgtgtat
     4501 aatatctcca acagaagata agttcatttc agttaaatct tcttctagta atcccttata
     4561 tcctctattg attccatata ctttgcatcc ataatatatt gctgatctta caaccgctct
     4621 aatcgcagca ttcattcctg gagcatctcc tccacttgtt agtaatccta tagttttcat
     4681 tttttacctc cttggatttt atgtcaattt tttattataa tcattgttat tgtattctaa
     4741 tggacttttt ttgtccctat atcacttttt aatcccatca agactaaaaa aattaaatgt
     4801 ccttaaatgt ccattttgtg atattatatc acaaaacgga caatattttt catattatta
     4861 taatatattt agatttttat ataaaaaatc aagcttttac ttttatgtta tctttaggaa
     4921 gtaactcttc taagttattt ataaattcat cacatataca agtcaatgcc ccggtcattt
     4981 tatatgcttt acgtttacca tttatatttt catccatagg gtataagtat atactgtcat
     5041 tacctggata ttgtttggct atttctacaa tattattagt tgtatcagta tcagacatac
     5101 tatctatttt caaatacaat tttttaccac tttctggctt aatattttct acatttctag
     5161 ttcttcctga attgtagttt ctatctgcaa agtcagtatc atcatttata tcttttatct
     5221 ctctagctat aagttttgca ttttcgcctt cttttatact taatgttcct tttacatata
     5281 ttatattatc ttcattcaaa atgacattat acttttgtaa taattgagga aaaactatta
     5341 tttctatagc accatacaaa tcttctaatt caataaatgc cataatttca tttcgttttg
     5401 ttgttttaat agttttagtt acaatcattc cacccattat aacatctgtt tcattcattt
     5461 gtaggtatga ctcttcttcc tcttttaatg aatttaattt tccattatcc atagatgttt
     5521 tttcttgtag ttctttttca aattgtgata aaggatgtcc acttacatac attcccaaga
     5581 cttctttttc aaggttaagt ctctctcttt cttcgtactc ttgcctttta ggcaaactgt
     5641 atatttgttg aaaatcacta tcttcctcac tatctccaaa agcatcaaat aaagaaattt
     5701 gaccttgaac atttttcttt ctatccatag atacactttc taataaactt tcataaccag
     5761 ccatcagact agctctattt tcacttatat tatcaaaagc tccacatttt attagacttt
     5821 ctattactct cttatttgta tctttttggt ctaatctttt gactaaatct actaaatcta
     5881 caaataatcc attagattct ctttcagcga ttatattgtt tattatattt acgccaacat
     5941 tttttacggc tgctagacca aatcttatat tgtcgccttc aactgaaaac tttgaaaaac
     6001 ttttgtttat atctggaggt aatacatcta tttctagtgc tttacattct cttatatact
     6061 caactacctt atcagtattt cccattacac tggttatcaa tgctgccata aattcaactg
     6121 gataatatgt ttttagataa gctgtctcat atgctaaaac cccatatgca gctgcatgtg
     6181 atttattaaa tgcatatcta gcgaagtcta tcatatcatc aaatatttta ttagctattt
     6241 cctcttctac accatttctg atacaccctg caatttctat attaccatct tcatcaaatt
     6301 taccatgaat aaaatattgt ctttcttctt ccatcacgtc catctttttc ttactcatgg
     6361 ctcttctaac taggtcactt cgtccgtaac tataaccacc taaatctcta acgacctgca
     6421 taacctgctc ttgatatacc aaacaaccat atgtaacttc cattataggc tttaattttt
     6481 catgtagata tgttacatct tttgggttgc ttttattttt tatatatgca ggtatagaat
     6541 ccattggacc tggtctaaat agggatattc ccgctacaat atcttcaaaa ttgtctggtt
     6601 ttagctgttt cataaaactt ctcatgccag cactctccaa ttgaaacacc cctagtgtat
     6661 ttcctgatga aagcatttca tatactttcg ggtcgtcata atccatcttt gaaaagtcta
     6721 ttttttctgt gtaatttttt ataactctat ttttttctat taaatcaaga gtatctctta
     6781 taactgtaag agttcttagc ccaagaaaat ccatctttaa aagcccaagt tcttcaagtg
     6841 ttgtcatagt aaactgtgtt gtgattgcat cttgatgttt gtacaaaggt acatattcat
     6901 ctacgggatt ttttgatata acaactcctg ctgcatgagt agaagcatgt cttagcattc
     6961 cctctatctg ttttgatata tttattattt cttttgtttc cgggtcttgg tcatacaatt
     7021 ctttaagatt aggatttgta tctagggcct tatctattgt cattcctaaa gcaaatggta
     7081 tttccttggc taccttgtca actttattgt agcctatatc caataccctt ccaacatctc
     7141 ttattgcagc ttttgcaccc attgttccaa aagttattat ctgggctaca tggtcatcac
     7201 catattttct ctttacataa tctataactt cttctcttct ctcataacaa aaatctatat
     7261 ctatatctgg catggatata cgttctggat ttaaaaaacg ctcaaataat agagagtact
     7321 ttattgggtc tatatcagtt attttaagtg tatatgctac tatcgaacca gccgcactac
     7381 ctctacctgg tcctactata atattatttt ctttagaaaa atttataaaa tcccaaacaa
     7441 ttaaaaaata ctctacataa cccatttttt caattacatc taattcataa tttaatctct
     7501 ctagtatctc attactagga ttatcatatc tctcttttaa tccattaaaa cacaactccc
     7561 ttaaataagt ttctggtgta tatccttctg gaacatcata cttaggaaga tgtatagtat
     7621 taaaatcaaa ctccacatta cacatatcag ctattttcac agtattgtca atagcctcta
     7681 atgcatatgg aaataattct tccatttctt ctcttgattt taaataaaac tcatcactac
     7741 caaatctcat tctatttggg tcatttaatg tttttcccat ttgaatacac ataagtacat
     7801 catgtatttt cgaatcttct ttatttacat agtgaacatc attagtagca actagtggta
     7861 taccagtctc ttttgataat tttactaaac cagagtttac ttctttttgc tcaggtaaat
     7921 tgtgatcttg aatctccaaa aagaaatttt cttctccaaa tatgtctctg tagtcaagag
     7981 caaattgttt tgctttttca taatttctat tcattaaagc acgtgctaca tctcctgcaa
     8041 ggcatgcaga aagtgctata atccctttgt tatgtttttt aagttcttcc atgtcaactc
     8101 taggcttata gtaaaaacct tctacgtatg aagttgaaac tatttttatt aaatttttat
     8161 atccttccat attcttagct aacagaacta aatgtccttg atatttgtca tagtttgggt
     8221 ctttatctct caatccacgt gcagctgtat agacttcaca ccctataatt ggctttattc
     8281 cttcttttat agctgttttg tagaaatcta taacaccaaa catacaacca tggtcagtta
     8341 tagctattga gctcatgttt agttccttag ccctttttat aagttttttc accctcgaaa
     8401 acccatcaag taaactatac tcagtatgta catgaagatg agcaaaatct ttgttcatta
     8461 aatcacctcc tgctttataa ttttaatttt accacaaata tatgtcctaa aacaaaaaca
     8521 gagactttta tctcaaaata ggaattaact tcaacatata ccattatatg ttataaatta
     8581 atgcttattc tgagattgtc ccctcaattt attgctatga tttttttttc aaatctgttg
     8641 caatttcttc tatttttcta agtctatgat tgactccaga ttttcctatt ggtggttcta
     8701 acatctctcc aagttctttg agagtcatat cctcatactc gattctaatc ttagctatct
     8761 cttgtaaatt ttctggcaaa ctgctaattc ctatagtctc ttgtatgagc ttaatatttt
     8821 ctacttgtct tactgctgca tttactgttt tagaaaggtt agctgtctca caatttacta
     8881 tcctgtttac attatttctc atttctttta ctattttagt attttgaaga cttaaaagag
     8941 cgtggtgtcc tcctataata ctcaacaaat ctgatatttg ttcactctct ttgatataaa
     9001 caacgtgatt attttttcta gccacaattt tactattaaa acctaaagag tttataagtt
     9061 cttttagaga atctgcaaac tcctcattat tagttacaaa ttccatatga tagttctttt
     9121 ctgggtcgct tatagaacct ccaccaagaa aagctcctct tatgaaagct ctcttacatc
     9181 cttcatgctt aattaagttc tctgaaattt tgtttatagt aaaaaatcct tctttatctt
     9241 caagtatacc taactttttt aataacattt ctgaacccat ttcgcttgtt accattaata
     9301 cataactgtt atttctcttt agcatttgat tcttatttac tgagattgta gtattgatat
     9361 taaaattctt ttttaataat ttaaatactt tacgggctat tgaatttaac tctgttgtta
     9421 tctttagatt tagcttctta tatccaacaa tttgtatact tccccctgat ttaactagag
     9481 ctgacaattc agctatatta caacattcat tcccagatat aactcttgct agttcatttt
     9541 tggtttcagt tgaaaaagac atatctatca cctattaact aatttttctt taaccttttt
     9601 tttatttctt gtaaggcaac ctttattatc tccttttcat catcatacct agctaagtct
     9661 actactctat ctaggtggat aaatttagca tcaataaagc cttcctcttt ttgtggaata
     9721 gtatccatat tctttgcttg catcaaatac caaaaaactg ttttatgaac tgctctattt
     9781 tcatcccagt tttctttgaa tgtatagtgt atttcaccta aatatttcaa tatatctgct
     9841 tttactccag tttcttcact tacttcacgt aatgctgcct cttggttatt ttccccttct
     9901 tctacttttc ccttgggtaa cacccaatct ccattgaact tccttagtaa aagaatcgta
     9961 ttgccaaaca ggactacacc accagcacta acctcttctc tcatcgatgt tcactcctta
    10021 aaactaacat aatgataagg atttctacac aattttttaa aatccttttt tactaacttt
    10081 tgttgtaatt atgatttagg gctaactcaa taactatgtt agatatatac tttgcatcat
    10141 gtcttatata attatttttt atttcaatta aattcttctc tacagttttt atacccatct
    10201 cttttaattt atctttttgc tttttatcta acaagacttg ctctgctcca tcttgtttat
    10261 ataagtcaag catttcttct ggcaatatct cattatttgc gattacataa tctattaaat
    10321 tctcatcagt gtgttttact atagcattaa catgctctaa tacattgtac ccttctgtct
    10381 cacctggttg agtcattata tttgctatat atacttttgg agctacagac gatttaatag
    10441 cgtctacaat cccctctaca agtaaattgg gtattatact tgtatataaa cttccaggac
    10501 ccataattat aatgtcagca tcataaatag atgcaataac atcctttaat ggttttacat
    10561 cttttggatt taaaaaaatt ttatcaatag agcttttttg aatttttgac tcttcaggta
    10621 tactagattc tccatttatt atattgccat tttctaattt ggcaactaaa tttacatctt
    10681 ccaaagttac tggtaaaact cttcctgtta tagcaaaaat ttcacttaat ttatatacag
    10741 ctttttcaaa gtttccatac aacccattca tagcagccaa aaacaaattt ccaaaactct
    10801 gaccttttaa aagcccttct gtaaacctat attgcataac ttcattcatt gttggctcta
    10861 tatttgcaag tgctaataaa cagtttctta tgtctcctgg tgggagcatt cctaagtctt
    10921 ctctcaatac acctgaacct ccaccatcat cagcaacagt tacaattgca gttatatttt
    10981 gcgtagtgtg tttaagacct cttaaaaata ccgattgacc agtacctcca cctatcacaa
    11041 ccacatttgg ttctctattt tcaacaatag acctttgaaa tagttttact ttttcatttt
    11101 ttgctttttt atatataaat aacgatacaa ttaatgcaat ccaacctaaa attcctacaa
    11161 taataagtaa tttagccatg tttgcctcca ttatcgcaac atagagtctc tatgtttctt
    11221 tacaactcta tatcccttat tagataaatc ctccgctata agatttgtta tagtaactga
    11281 cctatgtctt ccacctgtgc atccaacacc aattactaga tgttgttttc cttcctctat
    11341 atattgaggt actaaaaaat gaatcatatc taaaagtttc acataaaatt cttcactgat
    11401 tttagaattc ataacataat ctctaacttc tttatcatca ccagttttag ctcttaactc
    11461 ttcaacataa tagggatttg gaagaaatct tacatcaaat actaaatctg catcagcaag
    11521 tataccatgt ttaaatccaa aagatactac agaaatagtt aggtttggat tatcttcacc
    11581 tgaagaatat attttcttta tttcttcctt aaggtcttta ggtttcatgt tagttgtatc
    11641 aattatacat gtagataact cctttaaagg ttccattatc tttctttcca tttttatacc
    11701 ttctggaatc tgcatatctt ttgccaatgg atgatttctt cttgtcattt tatatctctt
    11761 taacaataca tcatcattac aatccaaata taccatttca tattcataat tttctttttc
    11821 taaataattt aagctttcat gaagcgcttc aaaaaacttt ctccctctta tatctatccc
    11881 tagagcaact ttatcaatac ttgaatttgg ttgataacat aattctgcaa atttagttat
    11941 caaagcagga ggtagattat ctacacaata aaatcccata tcctctaaag ctctcattgt
    12001 ctcacttttt ccagatcctg aaagacctgt aactataaca aatttcattg ctaactcctc
    12061 ccttttatct tatgtttatt atattataat gttttctact aacgatatat ccagattaga
    12121 gtcagatata gtcttcatag ctagttcaaa atcaccaact ctttctggac aatgtgcatc
    12181 tgagcctatt ataaatttat tttctagccc ttttatttta tttaattgat ttacattcaa
    12241 aaaagaatgg ctactattta tctcaagtct agtatttgtc ttttttgctg tctttgctac
    12301 ttcttctata tacacatcgc ctttatctcc tggatgagta attataaaaa tatcattatt
    12361 acttatcgca tttattacag cttttgtatt atattctttt gctttttcag ttttaaaaat
    12421 atagttatta caatgattta ataagcttct taatgatgtt ggagtagaac caaaatgata
    12481 tccagccata atataatcaa aatctctaat aaatttatca tccatatcta tatttccttt
    12541 atcatctaat atgttacact ccatacctaa aagtatattt atatcagtgt acttatcatt
    12601 taaataatct acttcttctc tcattttgag aatatctttt ttctttacac catatgcaat
    12661 atgtttataa ctatggtcag atattcctat tgtttttatt cctttagaaa tggctacctt
    12721 tacattatcc tcaatagtcc ctttgccatg actatataat gtatgtgtat gataatcagc
    12781 cagtattttc atttgttaat cctctccaag tattttgact tcttcttcca acataacccc
    12841 aaacttggca tatacagcac tttttataac atatattaaa tccaaaatat ctttagcact
    12901 tgcttctcct tgattgacta caaatccaca atgcttctca gatacttgtg ctcctcttaa
    12961 agtaagacct ctcaatccag tttcttctat taatttacct gcaaaatatc ctgttggtct
    13021 cttaaatgta cttcctgcac tagcaaaatt taatggctgt ttagttattc ttctcgtcgc
    13081 taaatcatcc attaaacttt ttatttcatc ataatttcct tcttgtaatt ccaattctgc
    13141 tgataaagct atatatccgt ttttcgatat tatacttttt ctatacccaa attccatctg
    13201 ttcatttgat aattcaaata tattgccttc catatccatc agtcttactg acttcactat
    13261 gtccttcatt tcaccaccat atgctccagc attcatggct aatgctcctc ccaatgtgcc
    13321 aggtattcct gatgcaaact caaacccttt taattcttgc ttttggagag ctttccctaa
    13381 aactgataat aaagctcctg actgagcagt catcctagta tcatttatct cataagaatt
    13441 gaaattatca gctagttcta ttaccactcc tcttattcca ccatctttaa ttaaaatgtt
    13501 tgaaccattt ccaattatta aatacggaat agattcttct tttaccaact taagtacatt
    13561 ttttaattgt tcttcagttc taggtctaac taatatatct gctggtcctc ctaccctaaa
    13621 tgaaatatgt tttttcatag gttcatcgac ttttatatcc tctttactta atatatttaa
    13681 taaatttata tatatatctt gattattcat tgtatttcct cccaaattat actgaacttt
    13741 agctgtcatt attatttaaa ttacattagt atatagtatt aactatattc aataatatat
    13801 gattttgctt atcatatgga ttttatattt taagcttctt aattacaaac tatattcaat
    13861 aaatatataa taaagtaaaa aaaaaaaaat aacaacatat aaaagaatat aaataattta
    13921 aaaaaagcat caatatcact gatatcaatg ctttaaaagg caatttaatt cgtattattt
    13981 atctaactat ttttgacata gcaaagataa taaatccctc caactactat ggaacctcca
    14041 acgatatttc caagcgttac aaataccaaa ttagttataa attgaaccat agttatattt
    14101 gctcctaatg ccattcccat tggtataaaa aacatgtttg caacactatg ttggaatcca
    14161 cataatacaa atagcattat tactgtccaa catgaagcca attttgacat tatatcttga
    14221 gatgctgtag ccatccatac agctaaaacg actagaatat tacaaagtat tgcttttaaa
    14281 aacattgatg ctatagttga attaacctta gcttctgcca cagctatagc ttttgtagct
    14341 gcatctcctg tcaatgtaga agaataaaac acaagtgcaa ctagcaaaat tgaacctact
    14401 aaatttgcaa aataaaccag tccccagttt cttaacattt ttcctaatgt tatttttttg
    14461 tccataagtg caatagccat taaggtgttt ccagtgaata attctgctcc tgccactact
    14521 acaagcataa gacctactgg aaagaccata gcacttaaaa agctagctac ccctgggtct
    14581 atcttgccaa aagtttgact aactatcata ctccccaatg ccccaaaacc tataaatgct
    14641 cctccaaata aacctaatag aattgcgttt tgtgtcgaaa tatttgcttt tttaatgcct
    14701 gcacttatag ttgcttgtgc agtttcccca ggtgtcaaga atcctttact cataaatcct
    14761 tcccccttaa aaattatact ttcccttgtc tttttatggc aataattata taataacaat
    14821 atgatatata ataaatttta ttaacataaa aaatatacta tatacttata tatacttgtc
    14881 tcatataatt atactattta ttatataaca actttgttaa tttcgaaata atttatattg
    14941 aatttgaaaa atttaccttt tgattattta ttttactttt taattattga ggtattatat
    15001 ataatagata tatgtttgtg ttattatata taattactta ttttaataaa tatttgaagg
    15061 agggaatttt atgtttgata tatcattttt cgaaatgtca atatatgaaa tagcagttac
    15121 tacaatatat ataattaatt tcatggttat actaaacctt atctttagag aaaaaagaaa
    15181 cataaataca actttaacat ggcttcttat attagtattg attcctgctc ttggattcat
    15241 actttatata gcttttggca gaaatatttc aaaaaacaat atgtttagat tgaaagaaaa
    15301 ggatgataag ataatcaaaa gtaatatact agatactcag gttaaactcc aatctacatc
    15361 tgaaattgat tcagatatac atcaacataa ggatatgatt tatgcattgg ctaattcaaa
    15421 taatgctcat tataccaata ataacgatgt gtggatttat gctgaatcta gtcaattttt
    15481 taacagtctt cttgaagaac ttaaaaaagc taaaaaatat ataaacatac aattttacat
    15541 tttcaaagat gacaaaatcg gaactgaaat aatagacatt cttatagaca aagctaaaga
    15601 aggtgttgaa gtaagacttt tatttgatgc agttggtggt agaacactaa aaaatagtac
    15661 actttccaga cttaaagaaa gtggtgttaa agttggaagc ttcttcccat cattcttgaa
    15721 aattgtaaac tttaacttaa actatagaaa tcaccgaaaa attgttgtca ttgatggtaa
    15781 ggtaggattt gtaggtggat ataatgttgg tgatgaatat ttaggtagaa atccaaaatt
    15841 tggtctatgg agagatacac atactaagct tactggtgat tgcgtaattg acctaaatat
    15901 gagattcatc cttgattgga gatatacaac taaagaagac ttagatttag aaaagtactt
    15961 tatagaagaa gatacaaatt ctaatcatcc ttctgaaaat attgggattc agatagtatc
    16021 aagtggtcca gatatatcag aacttgatga gattaaatat ggttacctta aaatgattca
    16081 gaaagctaga aagtatatat acatacagag tccttattta atacttgact caacttttat
    16141 agatacttta aaaatagctt gtttatctgg tgttgatgtg agagttatga ttccttctaa
    16201 acctgaccat ccctttgtat actgggcatc atattcatat gctggagaac ttttaaagtt
    16261 tggagctaaa atctatactt atggtcaaaa tgcattttta catgctaaga caatagttat
    16321 agatgactct atatgctcta taggaacagc taatatggat atcagaagtt ttgagcttaa
    16381 tttcgaagtt aatgcattta tgtactcttc aaaaaaagca ttggaacaaa gaattatatt
    16441 tgaaaatgat attttaaatt ctaaggaaat aacattagat atatataatt ctaggtctac
    16501 ttatataaaa atcaaggaat caatttcaag actattatca gcagttttat aaatctttta
    16561 ttttataaaa attttacatt ttggtttatt ttttaaagtt ttttatatgt attatttttt
    16621 ttttattgaa tttagctcat tttttttatt ttttttaact attctgaatt attttatatt
    16681 ataatagact agtatagttt ttatttttaa taggtaactt ttttgcattg tttttataaa
    16741 taaagaaatt gttgtatatt attctttctt tattgttttg gttttgttca cccacataat
    16801 gttggggtca actcatggtt aattatttta tgaaaaataa taactttaaa aagaaaaggg
    16861 ggatttgtta tgtgcgattt tatttcacac aacaagcaat tatttgacga actagatata
    16921 tttattgact ctcttgcaac aaaggaaggt gcgttaatac aagttctaca tgaagcccaa
    16981 ggtatatttg gatatcttcc aaaagaagtt caacttcatg ttgctagaaa acttggagta
    17041 gctcctgcaa aggtatatgg ggttgttaca ttctattctt attttactac agaacctgta
    17101 ggtaagtata aaatcagtgt ctgtctagga actgtatgtt ttgtaaaagg tgctgacaaa
    17161 atcttaagtg cttttgaaaa acagcttggc ataaaagttg gagaaactac tagtgatttt
    17221 aaattttctt tagaaggttt aagatgtttg ggggcatgtg gtttagctcc agttgttact
    17281 gtaaatggta aagtttatgg taaagtgaaa cctgaccaag taagcgaaat attagatact
    17341 tatagagaat tagaattgaa ttgttaagag gggtgaataa ataatgcgta aagtaaattc
    17401 atttgatgaa ttaaaagttt tagctgatga gctgaaacct aatcttagct taagaaaaaa
    17461 ttattctaag gaaaatgtat taagaagaga attacttgtt tgttgtgata ctggatgtac
    17521 atcttcaaac agtctagaaa tagttagtga attagaaaat gaaataaaga aatcaggaat
    17581 ccaagataaa gttagtgttc gtttgacagg ttgttttggt ttctgtgctc aaggtcctat
    17641 tgtaaaagtt tatcctgata acgttttcta cgtaaaagtt gagccatctg atgctgaaaa
    17701 gatagttcaa agccacttaa ttagaaatac agttgtcgag gaattattat atgaggaaga
    17761 atctttaagt aaaaaagttg aagaacaaga agaaatgtct ttctacaaaa aacagttacg
    17821 tatagcttta aaaaattgtg gtcttataga cccagatagt atagaagaat atattgccaa
    17881 tgatggttat ttagctctag gcaaatgttt aacttctctt actcctcaag aagttattgc
    17941 agaggtaaaa acttctggcc ttcgtggtag aggtggtgca ggtttcccta ctggctctaa
    18001 atgggaagct gcttcaaaaa atccagctgg tccaacagat aaaaagtttg ttgtatgtaa
    18061 tgctgatgaa ggtgacccag gtgcatttat ggatagatct gtacttgaag gtgaccctca
    18121 tagtgtacta gaagctatgg caatatgtgg atacgctata ggttctgata caggatacat
    18181 atacataaga gcagaatatc caaaatctat agaaagatta aaagtagcca tagctcaagc
    18241 tgaaaaatac ggtttacttg gcgaaaatat cttaggcact ggatttaact ttaaattaga
    18301 attaaaatat ggtgcaggtg ctttcgtttg tggtgaaggt acagcgctaa tgcattcaat
    18361 tgaaggtaga cgtggtgaac ctagaatgaa aacttatagt tcttctaaga gtggtctatg
    18421 gaaatctcct acttgcttaa ataacgttga gacttttgct aacatacctg caattatact
    18481 taagggtgga gattggtttg ccaacttagg tactgaagat tctagtggta ctaaagtctt
    18541 tgctcttggt ggaaaagtag aaaatgtagg acttgttgaa gttccaatgg gtactacttt
    18601 aagagatatt gtatttgaaa ttggtggggg aatccctgaa ggtaagaaat ttaaggctgt
    18661 tcaaacaggt ggaccatctg gcggatgtat accaactgaa catttagaca ctcctataga
    18721 ctttggttca ttaagttcta tcggttcaat gatgggctct ggtggtatgc ttgttcttga
    18781 tgaatctgac tgtatggtag atatagccaa gttcttcctt gagtttactg ttgaagaatc
    18841 ttgtggtaaa tgtacacctt gtcgtatagg tactacaaga ttattagaaa tattaactaa
    18901 aattacagaa ggtaatggta ctctacaaga tttagatgac ctagaaaacc ttgctgaaac
    18961 tatacaaact gcttcattat gtggtcttgg taaagctgct ccaaaccctg ttttaagtac
    19021 tcttcaatat ttcaaagatg agtatatagc tcatgttgta gataagaaat gtcctgctgg
    19081 taaatgtcag aacttacttt cttactttat aacagatgac tgtaagggtt gtacaaaatg
    19141 ttctagagtg tgtcctgctg gttgtataac aggttctgtt aaggaacaac atacaataga
    19201 tacttctaag tgtctaaaat gtggtgcttg tatagataat tgtacattta acgctataat
    19261 caagaaataa cttatttgga ggaggaataa caatgagttt agttaattta actattaatg
    19321 gcaaacacgt ttcagcacct agtggaactt ctatcttaga tgctgcgaaa ttaataaata
    19381 taaaaataca caacctttgt catttacata tgaatgaaat agataagctt gacacttgtg
    19441 cttcttgtcg tgtttgtatg gttgagacag agagaggatt agttccagct tgtggaacag
    19501 ttataaagga aggtatgaaa gttcagacta atagtgctaa agcattaaat gctcgtagaa
    19561 ctattgttga attattgtta tcagaccatc cacaagattg ttttgtctgt gaaaaaaatg
    19621 gagattgtga attacagact atagctgctg atttaggagt aagaaaaata agatatcaag
    19681 gttctaaatc ttttgcaggt aaagatactt ctactaaatc tttagttaaa gaccattcta
    19741 agtgtatatt atgtagaaga tgtgagactg tatgtaatga catacaaaca gtaggagcat
    19801 tatctggagt taatcgtgga tttaatactt tagttagtac attctttaac tctgatatgg
    19861 ttgaaacaga gtgtacattc tgtggtcaat gtatatctgt ctgtccaact ggagcattaa
    19921 cagaagtaga taatgtacct aaactttggg atgttttaaa taaaaaagaa aagactatag
    19981 tagtgcaagt tgctcctgct gtaagagttg ctattggtga agagtttgga ttagaaccag
    20041 ggtcaatctc tacagggaaa atggtagctg ctttaaaagc acttggattt gaacatgtat
    20101 ttgatacaaa ctttggtgca gattttacta taatggaaga agctactgaa tttatagaaa
    20161 gaatccaaaa aggtgaaaat cttcctatat taacaagttg ttgtcctgca tgggtaaact
    20221 tcttagagca caattatcca gacaagttaa acttagcatc tagctgtaag tctcctcaag
    20281 gtatgtttgg ttctatagct aaaaactact atgcacctaa aatattagga atcaatcctg
    20341 aagaacttta tgttgtatct gtaatgcctt gtgtagctaa aaaatatgaa gcttcaagag
    20401 aagaattaag tgaatctgga atactagatg ttgacctttc tataactact agagagttag
    20461 ctaagatgat aaaagaagct gcaatcgatt tacctaatct tcaagaccaa gattttgata
    20521 atcctctagg aaaatctaca ggtgctgcat ctatatttgg agcaagtggt ggagttttag
    20581 aagctgccct acgtacttca tatgagaaga taactaataa gactttagat aatgtaaact
    20641 tcactaatgt tagaggtctt aaaggaatta gggaagcaag tatagatgtt gatggaacta
    20701 cagttaatgt gtgtattgta aatacactta aaaacgctag aaagattatg gataaggtca
    20761 gaagtggaga atgtaaatac catatcatag aagttatggc atgtcctggt ggatgtgttg
    20821 gaggtgctgg tcaaccttat caccatggta atactgaaat agttgataga cgtgcaaatg
    20881 cgttatatga aatagataga aataaggcta ttcgtaaatc tcatgagaat cctgatttac
    20941 aagctattta taaagacttc tttggagaac caaatagtga tgtagcccat aaataccttc
    21001 atactcatta ctttgataag agttgtgtct atggagagtg tccacaagaa tgtgcttgtg
    21061 aagaagctaa gtagtcataa caaaatataa aatttattct taaaaaagga ttgtcttaaa
    21121 atacttttac gtaatttaag acaatcctta ttaatttcac aaattaaatt atatttctaa
    21181 atagacttat tctttaatta acctatctat taattaaaga atacctgcca ttctcataag
    21241 ttgttttgca tttgtagttt tacatcttcc atcttttatc ttatagtcaa aatgaatttt
    21301 atcgtctaca tagtactctg agaaatgata gtttacaact tcaacatctc cactacctga
    21361 aaggtcacat agctcaaagt catgtgttgt caccatactt ataacccagg gttttgataa
    21421 tttctttact gcctcacttg ctccaataat tctatcagca gagttagttc ccttaaaaat
    21481 ttcatctact agaaccaaca ttggcagttc ttttaaacta tattgaatca ttgattttat
    21541 tctaagaatc tcagcataga aagttgatat tccttgactt acatcatctt gtattctcat
    21601 agaagtaaat atagccatac aggtcgcatc aaaactctta gcgcatactg gagcacctgc
    21661 atatgccagc actaagttta caccgatact tcttaaaaat gttgtctttc cagacatatt
    21721 tgaacctgtg ataatacaag tctgagaatt taattgtatc gaatttgcta tgacactttt
    21781 ttctgctatt aatgggtggt atacctcttc aactttcaat cttggagtat cttcatattt
    21841 tattactgga aaacatgtat tttcctttac atgagaaaca actgttaaac ttattagagc
    21901 ttcaaattct ccaatagatt caaaccaatc tctaatttga tttccatata tacctttcca
    21961 tcgctccaaa gcttctgcac aatgatagtt ccacatcaaa acaccacagg caattatata
    22021 tagtatctga ttatacctta ggtttacagc atttcctatt gaatttaatt gtttaacacc
    22081 tcttgaaact ccacttccct tagaaagttt atcttgtaat tctttcaaat atgcactctc
    22141 aaacgtctct ttttctaaaa cctcaaacat tttttcatat acttgtatac ttctactaaa
    22201 tgaaaataat ggagcaagag tttgcataag tcttggatat ccaaaaccac taaaccctaa
    22261 ctggattata aaaagtaaaa aaacaggtaa aacaggtaaa aatcctaaca tacaaaatat
    22321 aaaagataag attgttgcag ttggtaaacc ccaagtaaaa atatgcatcc attttggtat
    22381 atacacattc ttattttctc catattctat aaacgattct atactactat ctgaattgtt
    22441 tttttgctct ttaccaatta tattactgag tgtttgaata tgaatagaaa aatcatcttt
    22501 tgataaaagt tcttttatag ctctctgtct ttctaagatt acattctcat ctggattttc
    22561 attccacaaa tatttggcta aaaaattttt cccataagat gtattagcta cacagatata
    22621 ttgatacaat gatgctcttc caaataagtc taaatctttt gcttgcgaat tttcatcttt
    22681 taagtattct ttaccatcta ttttaaattc cttccaacca tctccaaatc tcttaatata
    22741 cttatttaat acttcgctct tacttttaaa atatgtttct tcttctttta ctttactatg
    22801 aataactaag agtgctataa atattattat tgataaaact ccaacaaaaa tacctactat
    22861 atttttattg taaaatccaa tagcaaaccc tgctatcact atcaaaaaag atattaatct
    22921 tgctgtactt atgttatttg actttttact taagagcaat tctttttcag tactactttc
    22981 taaaagttta ctaaaatatt ctattcccat aaaaaattcc aactttcttg ttatataatg
    23041 aaactcaaat ataaaattaa tctagtatta ttataatcta acactgtact ttaaattatc
    23101 cttatataat atatcttatt aataaacatt atatatatac aacttgattt taatttataa
    23161 ggtgtaggac ataatcctaa tcaatattta attaaatgat gtgtagttca ctattattca
    23221 gtactactta gtatcgatta attttgataa ttttttagta aattcttgag ctgcatcgta
    23281 tcccattcct ctttgtctaa aattcattgc tgctacttct aatatcatag cagtatttct
    23341 tccaggttta acaggtacaa ctaatttctc tacattaaca ccaagtattt cttcatattc
    23401 tttatccagt cccagtctat cataatactt agtttcatcc caagcttcta aatgaactac
    23461 taaatctatc attttagaat ttttgattgc ccctactcca tataaacttc taacatctat
    23521 tattcctatt cctcttatct caagaaaatg tcttattagt tcaggtgact gacccacaag
    23581 catactttca tctaattttc ttatttctac agcatcatct gcaactaatc tattacccct
    23641 ttgaatcaat tctaatgctg tttccgactt tcctatacta ctttcacctt ttatcagtac
    23701 acctatacca taaatatcta ctagcacccc gtgaattgtt gtgtgtggtg ctaacttatt
    23761 atccaaatag tttgacagtc tatttataag tctagtggta tttctatttg tgctcaaaag
    23821 tattctttta tattttttag ccttttcaat tacatctggc ttgatttcca agtctctaga
    23881 aattattaac gctggtattt catatgagaa aaatctatct agtacttctt cccgaatatg
    23941 actatccatt aatccaaaaa aattatattc tgtctttcca actatttgta ttctttcata
    24001 tgcaaaataa tcaaaatatc ctgcaaactg taatcctggt ctatttatat cttgagagta
    24061 gacataataa tctacatcat caggcatgta tactactttt aacttcaact cttttattat
    24121 ctctcttatt gatactttat tgctagtttc cattaatttt cttcctttct aaaaaaatta
    24181 taaacattat ctgcaatgct tttattcatt ccatctactt ctaaaagttc ttccatagta
    24241 gcttttttta tagcatctac atctttaaag tgatttaata atgatttctt tcttttttca
    24301 cctattcctt gtatatcatc taagatagat tttgtcaaag ctttatttct tagacttcta
    24361 tgatatgtta ttgcataatt atgaacttct tcttgtatac tagcaataaa tctatataaa
    24421 ttagttgttt tatctagttc tatttctttt tctttgcata tcaatccttt agttctatgc
    24481 ttatcatctt tgtacattcc ccaaagaggt atatctacat cattgagttc tagtactttt
    24541 tttactgcac taacttgtcc ttttccacca tccaacaata tcaagtcagg taaatttcca
    24601 tgttttaatc ttctatctac aatttcagcc atagaatcat aatcattagg tccaataact
    24661 gtctttattt tatatctcct atattccttc ttatcttttt tagcattcgt atacactacc
    24721 atagaaccaa tcgaatctac accttgtata tttgatatat cataagcctc tattcttatt
    24781 ggcaactttt ctagatttaa tatttgcttt aattcttcca atgctccaat acttttttca
    24841 tattttcttt tattcatatc agaaaatttt tcaagatatt ctacagcatt tttcctaacc
    24901 atttcaacta agcttttctt ctctcccttt tgaggaactc ttattgttac tttttgcccc
    24961 tttttagatg acagccattc ttctaatata gaactatctt ctatctcatc ttctataatt
    25021 aattctttag gtatatactc ttgttcatta taaaattgtt ttacaaatga acttaatata
    25081 gaagctcttg gactatccat taccccttct aatataaaat gttccctacc tacaatctta
    25141 ccatttctta taaaaaatac ctggacacaa gcttcattat gagctcttgc cattgcaacc
    25201 acatcttggt ttaagtcact tacagtagca tctatttttt gtttctgtat catctcttca
    25261 aggctcttta tcttatccct atatacagca gcctcttcaa atctaaagtt catagaactc
    25321 tcattcattt tttcctttaa aagttccatc aatttttctt ctttaccaga taaacacatt
    25381 ataatctctt ctatcatttt tccatactct tctttagaaa catttcctgt acaaggtcct
    25441 aaacatttat ttatgtgtag atttaaacaa ggtcttgtct tattctttat tgctttatct
    25501 atatctattt tacagcttct tattggatac gtactactta taagctccaa ggtatcattc
    25561 acagcagtta tattggtata gggcccaaaa tactttgcct tatctttaag aactcttcta
    25621 acttttaaga ttcttggata atcttcattc gtagttactt ttatatatgg ataagtttta
    25681 tcatctctaa gcactacatt atactttggt ctgtatcttt ttattaaatt acattcaagt
    25741 attaatgcct ctaactctga atccgttata atatactcaa acttatatat attcttaacc
    25801 atagatttta ctttagatgt atggtttttt gaagattgaa aatactgcct taccctattt
    25861 tttagagata tggcttttcc cacatatatt atatggtcgt atttgtcttt cattaaatat
    25921 actccgggct cagaaggtaa tttttttaaa tgctcttgta tatcaaacat ctcgccctcc
    25981 aaaacattaa attttatact aaaattatac cctaaaatat gaattttaaa aagttataca
    26041 atttccagtg ctttaaacaa gttaaagccc atattccctt ctataattga tttgaaaaca
    26101 attttaacca tgaagagtaa tatggacttt actttgtatt actatttaca acttatattt
    26161 gactaattta cttataacaa aacagtttat cgtatatctg ctctaaaatt tttcacaatt
    26221 tatataactt aagaaaaata tttctttaag aattgacctg tataagaacc atcaacttta
    26281 gatacttctt ttggtgttcc agttgcaact atagttccac ccttatctcc gccttctggt
    26341 cctaagtcta ttacataatc acaagtcttt ataacatcta aattatgttc tatgactata
    26401 attgtatttc ctgtatctgc tagtctttgt aatacttgga ttagcttgtc tacatctgcc
    26461 atatgaagtc cagtagtagg ttcatctaat atatacaaag tctttcctgt tggtctttta
    26521 ctaagttcag cagctaattt tatacgttgt gcttctcctc ctgataattg agttgaaggc
    26581 tgtcctaatt taatatatga aagtcctaca tccattaaag tttctaattt tctttttata
    26641 tttggtatat tttcgaaaaa ctcaacagct tcttccacat tcatatctaa tatatcagat
    26701 atagttttat ctttgtattt tacttgcaat gtttctctat tgtatctttc acctttacaa
    26761 atttcacaag gaacatatac atctggtaag aaatgcattt ctatttttat tattccatca
    26821 cccttacaag cttcacatct accacctttt acattaaaac taaatctacc ttttttataa
    26881 cctcttgctt tagcttcatt agtagatgca aaaatatctc ttataaaatc aaatacacct
    26941 gtataagtag caggatttga acgaggtgtt cttccaatag gactttggtc tatatttata
    27001 actttatcta tactttcaat tccttttatc tccttatgct taccagctct ttgcttaagt
    27061 ctattcactt tacttgcaac acctttgtaa agtatatcgt ttattaatgt actttttcca
    27121 gaacctgaaa cacctgttat gcatgtaaac ttacctaatg ggaactttac atcaatattt
    27181 ttcagattat tttctgatgc tttaactatt tctataaaac ttccatttcc ttctctagta
    27241 gtctcaggaa gcaatatttg ttttttacca cttaaatatt gacccgttat tgagttctca
    27301 ttttcaatta tttcatctaa agtaccctgt gcaactattt caccaccatg tatacctgca
    27361 cctggaccaa tgtctataac ataatcagct tctctcattg tgtcttcatc atgctctaca
    27421 acgattaagg tatttcctaa atctgttaga tgtctaagag tagttataag cctatcatta
    27481 tctctttggt gaagccctat acttggctca tcaagtacat ataaaactcc aaccaatgca
    27541 gaacctattt gagtagctaa tcttatacgt tgcgcttctc ctcctgataa agttccagct
    27601 tttcttgata aatttaaata atcaagacct acatctctta agaacatagc tcttcctttt
    27661 atctctttta taatctcatg agctataaat ctctgttttt ctgtaagatt tatattttcc
    27721 ataaagtcaa tcaattcatt tacagataag tttgtaactt ccattatatt ttttccatca
    27781 actaaaacag ataaaacttc ctttttaagt ctcatccctt tacactttgg acatggtctc
    27841 tctgccatgt attcttcaat cttatctctc atgtaatctg aattagtttc tctatatctt
    27901 ctctctaagt ttactattac accttcaaag tatgatttat aatttctagt tcctccaaat
    27961 tttgactcat aagtaaactc tacaattata ttatctttac cataaagtaa ttcctccaca
    28021 aaatcatctg gtaaatcttt aaacggtgtt gtcaaagata cattaaaatg ctctgctaaa
    28081 ctttttatca ttttactgta ataagtgtca tcattaacac ctgttgaacc ccatgctgcc
    28141 actgcacctt gttttattga taaatctttg tttggtacta ctaattctgg gtcaacttct
    28201 ttactttccc caagaccatt acaaacatca caagcaccaa agggagcatt aaatgaaaac
    28261 attcttggag ataattcttc tatgccaatt ccatgttctg ggcatgcaaa tttagttgaa
    28321 aaaagaattt cttctccatc tattatttgg gctattacaa gaccatcaga tagcttaact
    28381 gcagtttcaa ttgaatctgc tagtcttgat tctattccat ctttaattac tattctgtcc
    28441 actacaactt ctatattatg tttttgattt ttatttaaga ttatatcatc actaacttca
    28501 tagttttcac cattaactct aactcttaca aatccttctt ttttgatatt atcaaagact
    28561 tttttatgtg tacctttttg tcctctaaca attggtgaaa gaatttgtaa tttcgttcta
    28621 tctggaaaat ctagcatttt atcaactatt tcttgaatag tcatctgact tattttttca
    28681 ccacatgtag ggcaatacac atctcctact cttgcaaata atagtctcaa ataatcatat
    28741 atttctgtta ctgttccaac cgtagaacgt gggttttttg aggtagtctt ctggtcaatt
    28801 gagatagctg gtgataaacc ctctatatat tctacatttg gcttttccat ctgacctaaa
    28861 aattgtcttg cataagcaga tagactttct acatatcttc tctgtccttc tgcatatatt
    28921 gtatcaaatg ccaaagaaga cttcccagac cctgacaaac ctgtaaaaac aataaattta
    28981 tctcttggta attctaaatc tatatttttt aaattatgtt cttttgctcc tcttattata
    29041 attttatctt ccataatgtt gtttttacct ttctattttt ccaatttttc ttctaattgt
    29101 ttcactttat ctctaagttc tgctgctctt tcaaattgta agttttgagc agcttccatc
    29161 atctctgatt ttaatttatc tatattagct cttatttcgt ctttatcatc tgtatcagat
    29221 attccatata caacttcttc ctctgcaacc ttagtagctt ctatactatc tcttatattt
    29281 ttctgtatag ttttaggaat gatattgtgc tcttcattat ataaattttg tatatctctt
    29341 cttctctttg tttcatcaat agcattttgc attgagtcag ttatcctgtc tgcatacata
    29401 attactcttc cattttcatt tctagctgca cgtccaatag tctgtattaa agctgtttcg
    29461 gaacgtaaga aaccttcttt gtctgcatct aaaattgcta taagtgaaac ttctggtata
    29521 tccaatcctt ctctaagtaa gtttattcct acaaggacat caaacttccc taatcttaaa
    29581 tctcttatta tttctgttct ttctaatgta actatatctg agtgtaaata tttaactttt
    29641 atccctattt ctttaagata gttagtcaag tcctcactca tttttttagt taaagtggtt
    29701 ataagcactc tttcattttt ttctatagtt ttagttattt ctcctaccaa atcatctatt
    29761 tgattattta taggtcttac ttctacaatt gggtctaaaa ggccagttgg tcttattatc
    29821 tgttctgcta ttgtctctga gtgttgaatt tcataaggcc ctggtgtagc acttacaaac
    29881 aacatttgat ttatatttcc ttcaaattct tgaaaattta atggtctatt gtcaagtgca
    29941 gaaggtaatc taaaaccatt ctctataagt gatgtttttc tagacctgtc tcctgcatac
    30001 atacctctta cttgtggtat agtgacatgt gcctcatcta caattatcaa ataatcatct
    30061 gggaagaaat ccattaaagt atatggtctt tctccttctg accttcctgt aatatgtcta
    30121 gaatagttct ctatcccctg acaaaatcct atttccttta acatttctat atcatactta
    30181 gttctttgtt caattctttg tgcttctaat agcctatcat tttccttgaa aaatttaatt
    30241 tgttcttgaa gctcgttttc tatttctaca attgcctttt caattctttc tggcgttgtt
    30301 acatagtgag atgctgggaa tataacaaca tgatttctag taccaacaat cttccctgtt
    30361 acataatcaa tttctgttat tctatctacc tcatcaccaa aaaattctat tcttattgct
    30421 ttttcatcat tactagctgg gaatatttct agtatatctc ctctaactct aaaggtacct
    30481 cgtgtaaagt ttatgtcatt tctttcgtat tgtatctcta ttaacctttt tattacatca
    30541 tctctgtctc tttgcatacc tggtcttata gataacataa gttctttata gtcttttggg
    30601 tcaccaagtc catatataca agatacagat gatattatta tagtatctct tctctctaaa
    30661 atagatgctg tagcagaatg tcgaagttta tctatttcat catttatgct tgcatctttt
    30721 tctatataag tgtcactgtg agctacatat gcttctggtt gataataatc atagtaactt
    30781 acaaagtatt caactgcatt gtctgggaaa aattctttaa actcactgta aagttgtgct
    30841 gctagagttt tattatgagc cattattagg gtaggtttct taacttgttg aatgatattt
    30901 gccattgtga atgtttttcc tgaacccgtt acacctaata atgtcgaaaa tttctcattt
    30961 ctatttatag aatccactat tgatttaata gcttctggtt ggtcacctgt aggtttgaag
    31021 tctgatttta ttttaaaatc catagtatca ctcctttttt cgaacattag ttcctgacat
    31081 gtattattat atcatacctt tataaaattg tttaagttat tttaaatatt ttacccattt
    31141 aaattttact taaatctaaa acataaaaaa agacttctat ctttgcaata gaagtctata
    31201 attcatggaa aataaatact ttattcaact tacataataa aatcaaatgc ttctttttca
    31261 actccatcta attttaaatt aaacattttt aatatagttg ctgcatgtgc tctcaggtct
    31321 ccttcttcta taaccacacc acctttaata tttggtccaa aagctataaa tggaggtcta
    31381 ttacctttag taggtaaatg accatgtgtc gcaacagcaa acttgtaatc ttttatatct
    31441 gaatctttta taatacttcc ttcgaaatca tttccaaatg aaattccttc aagaccttca
    31501 attaccattt caaaatcacc ttcaagacca aggttagatg cttcctcttt ggtaaaaata
    31561 ttctctatga ataattcttc tttaaattct tcaagcagtt ctcttaattc tgttaaccta
    31621 tctaagttat ttactattat ttgagctgat attccagaag aatttacata ggcatcaaaa
    31681 tccttaactt ctccattttc aacttttata aatccatttg atttaagcaa gacatttgga
    31741 tttataactt tatcaactct aagatgacca tggtccccaa gtattataaa atttgtatct
    31801 ttgtatgtac ctgcatcctt tgtagcctga ataatatctc ctaaccattt atcattcatt
    31861 tttaatgctt tatctacttc gtcattaaat aagccataat tgtgtcgtgt atggtccaat
    31921 gtagctaaat gtattagcat taaatctggt ttatattcct ttattatatc tactgaacag
    31981 tttactccaa ataaatccat gttgggttct aatttccaat cgatataatg acaatgtcta
    32041 ttgtaaatac cgtccattat aaccttactg gaacttcgtt caaaagtttc tcttgggtca
    32101 tcttctctac taggagccca tatttctgca atattataat ctatatttgg atttgctccc
    32161 attacaggcc ataatacact agaggtagtt agattatttt cttttgctac atctataata
    32221 gtttttgctt ttatgtcctt tgagtaccag taccagtctt tattttcttt atttgggtca
    32281 aatttttcat tatgatatat accatgtttt ttaggatata ctcctgtaac catactagta
    32341 tgacacggat atgtcagagt gggataaaca caatttatat tttttactat ggagcagttt
    32401 tctagtatag atttaaagtt tggtagattt tttacaaatt ccaagtcttt ttcaaataaa
    32461 gaatctactg ataatactat aacctttgtc atttacatta cctcttcatt tatatattat
    32521 tgaatacttt taatcaactt acaagtctat ataattattt atttgtttta tctattactg
    32581 cttgcattgc agaagttcct ttgcttacta catcttttgg atttgcattt aaatcagtaa
    32641 gccccttttt aatctcatcc attagcttat cagttgcttc cttgtatcca tttaacattg
    32701 gaagtgcaac agcatattgt aattggtcag ttgcaacctt atattgaggt ttttctttat
    32761 ataatgtttg taacttttct gagtttgttg catcttcagt tgttggtaag tatcctgttg
    32821 atgaactaaa ctgaacgatt ttatctgttt gtgtcatcca ttctataaat tctgcacttg
    32881 cttctttttc tttatctgtt ccaccttcaa gcataactag attagctcca cctgtaggag
    32941 ttgcgtattt ttgatttttt ggtaagaatg ctgttgatat ttcaaactta ccttctgttt
    33001 gttgcataag tcctgctaaa tctgctgttg atgtaaatat catacctact ttaccattta
    33061 caaaatcttg ttttgctacg tcccaggcat tataatcttc tccaggaggc attttcatta
    33121 ttccagcttt catcatatct tgccagaact taactggtgc ttgtcctgcc tcattattaa
    33181 atgctacttt tttaccatta gttatttctc ctccactttg cattaccata gcttcataga
    33241 accaaatatc tattggtgtt tcaaaaccat aagtatcttc tgatttattt gtcattttac
    33301 tagcataatc cttaagctct tcccatgatt ttggacctga agggtcaaga cctgctttag
    33361 ttgccatatc tttatttatg tataatattg gtgtacttct attaaatggc ataccaacaa
    33421 acttatcctc ataatatgaa tttgttaaaa gaccagaaaa gaatctttct tgatatgttt
    33481 tatcaaaata tttactcata tctactaaag cacctgctga tgcgaatgta cctgtttgag
    33541 ttatttctaa taatgttaca tctggttggt ctttagatgt taaggctgcc tgtagttttg
    33601 ttgcattttc atagtaatct ttctgtttta acatatttac ttgtacatcc ttatgttcac
    33661 tattaaattg gtcaaccatt ttttgagcag tttctccatt agttccacct aaaggagccc
    33721 agaaatctat cttcgtaata ttgctatcag tcttttctcc tttagatgca tttgcatcat
    33781 tagaatttga acacccaact aaagaaccta ataataatgt tattgctaat cctgttgcta
    33841 attttctttt aagcttcatt ttcatcctcc aaatttagtc tttaattttt tattttattc
    33901 ctgaatacat aaaagcttct tttacttttt tattcgcaaa tatataaaca agaattattg
    33961 gtaaaattag aattatattt cctgccatta ttacattcca tgcagtaatt ccttctgaag
    34021 atttaaggag cgcaactcct attggtaaag ttcttattgc atctgtattt gtcattacta
    34081 agggccagaa ataattattc caatggtata taaaattaaa taatataaat gttattactg
    34141 ctggttttgc cataggaacc attattctaa acattatttt taagtcagaa gcattatcta
    34201 gtttagctgc ttcaataatt tcatctgaaa tctgcataaa attctgtcta agcagaaata
    34261 tcccaaatga agatgtcata aatggaatta taagtggaac ataagtattt ataaatccaa
    34321 acttactcat catcatatag actggtagaa aagttacttg tggtggaatc ataagtccta
    34381 caagcaaaag cccaaatagc attttttcac ctttaaattt aatcttagaa aatgcatatg
    34441 ctgctggaac cataactaaa aattgtacaa ttaaaacact tcctgttaca aatacactgt
    34501 ttagcaaata tctaaaaaat ggacctgaat gccatgctgt actataattt tctaacacta
    34561 tatcatttgg tataatagtg ggcggtaatt gcatcatttc ttgtggattt ttaaaggatg
    34621 tagatagcat ccatataaaa gggaatataa atattgcaga taaaagtaat agaattaata
    34681 tttctattga ttttgatgct atttttttta tatcaacttt gtcttttttg ataggttgta
    34741 taatatccat tgtctcctcc ttttggataa accttataat atctactttt tctttattga
    34801 taatgaactt tctttgataa gagtctaaag ttagcatatg tagcaatcat aactatagct
    34861 aacaaaaaca tactcgctgc actcgcttgc cctatgtcat agtatataaa tccagactca
    34921 tatatataat aagataacat gttagttgaa cccactggcc caccttgtgt cattatactt
    34981 atctcatcaa atacttgaaa agatgcagtt gttgttgtta tcataagaaa aaatattgtt
    35041 ggtgataata gtggcaatgt cactttaaag aaattcgtta ttttgtttgc attatctaat
    35101 ttagatgctt catatatata gtttggtatg gatttaagac ctgataacac tattaagata
    35161 ttatatccaa gagtcttcca tattcctatt agaattagtg acattaaagc tgaactttca
    35221 cttgaaagcc actttgatgt tggtaaatgt aatgtcgata gtatccagtt taaaaagcca
    35281 tattgtggct ccataatcca aagccacaat acagctacag atactagtga aattacatgt
    35341 ggtgtaaaga ttatactttg cgttaaattg tgtattatag aatttttatt tagccaaaca
    35401 gcaagtacta tggctagtga tatagatata gtcaccataa aaaaagtata aattgcagtg
    35461 tttgttatag tttgtataaa ctctggattc tttaaaagtt ctgtataatt gtttaagcct
    35521 acattttcaa aggttgaact aagaagacta ccagatttga agcttagaga tatcgtctta
    35581 attattggat agactataaa caataaagtt cctataatag caggtgttat atataaatat
    35641 gctttattaa attgcttttt cataaatatc tcctttaatt ttagaataca tacttttaca
    35701 tacttttaca tcttctattc tttctccatt tttatcaaaa taatataaat ctttttctaa
    35761 tacacttatt cttaaagttc ttccaatatc aaatatatca ttttctgttt tgactcttat
    35821 ctctctatta agaacagata aacaatatat agtttcattt cccaacactt ctttagttac
    35881 aacttttcca aatagactaa atacatcatc attttttcca tctatgttta tactctctag
    35941 tgttgctttt tctggtctga atccaaattt aatatcattt tttatatcta gtatgttcat
    36001 acaaggactc cctataaatt gagctacaaa taaattttta ggattttgat atatcttctt
    36061 tggagaacct atttgcatta tctttccttt gttcattata actatcttat ctgccattga
    36121 cattgcctca acctggtcat gagttacata aacaaatgta gactttagtt tttgatgtaa
    36181 ttgtattaat tctgttctca tttgatttct taatttagca tctaaatttg acaatggttc
    36241 atccattaaa aatacctttg gatttttaac catagctcta gcaagtgcca ctctttgtct
    36301 ttgacctcca gataagttgc ctggtttcac atttaaatat tctctcaatc ctactacatc
    36361 aataacttct gttataagtt tttctctttt ttctttttct acttttttgt ttttaagacc
    36421 aaattctatg ttttctctaa cagtcatatg tggatataat gcataatttt gaaataccat
    36481 agctatatcc cttttactag catccgtttt tgtaacatca ttttcttcaa tcttcaacct
    36541 acctgaagtt atatcttcaa gacctgcaat cattcttaac gtagtagatt taccacatcc
    36601 acttgggcct accaatactg taaaagaacc atcttctata gtaagattta aattttctat
    36661 aactgtattt tttttattat aagattttgt tacattttct agtcttatat ctgccatttt
    36721 tatcctccca aaatatttaa tttaattcta cgatttactt atagattaat aatactctta
    36781 atttgtaaag gatatattaa gacaaagtga atatatggta aacttattaa aataaaggtt
    36841 aaattataaa cattttttgt taaatatact gaggttaaga tgatatacag cttatgttag
    36901 ctataatttt ctatattttt catattcatt gtttgctatc acaatgtaat tttaatttat
    36961 gaagagtcat ataaaactat ttatttttta tataatttga tttgaaagta aaaaagacct
    37021 ctatggggga tagaggtctt taagtggggg agattgagta atttattttt gctatattaa
    37081 gttttacaat tatattatgt cccaaattta caaggtttaa acataaaaaa taaaaatttt
    37141 ttaattttta taaatttacc
//
LOCUS       DAMKMI010000022        35800 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMKMI010000022 DAMKMI010000000
VERSION     DAMKMI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.023"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            112..393
                     /locus_tag="PC429_001047"
     CDS             112..393
                     /locus_tag="PC429_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846564.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVTNYLRFAALDNDMKIKL
                     FQTIIEAVARKKVSLPNDSEFTEGILQSSELEMTTNVGFIIEGLSYHNN"
     gene            complement(638..1114)
                     /locus_tag="PC429_001048"
     CDS             complement(638..1114)
                     /locus_tag="PC429_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846565.1"
                     /translation="MNKQKAKRFLRAIDMNIDKIEEEATKIYKERYLIETTDAIRISI
                     NLEGVVKSTISSWQGYSDDIFNMREVIVYEFSQEKVQIDDFLGELCFLNDYEEFATWC
                     DTESETLNWNSYEKFNKENFDELVERNIEDGLPIFLKELSESIENCKQDLKLMIEI"
     gene            complement(1154..1795)
                     /locus_tag="PC429_001049"
     CDS             complement(1154..1795)
                     /locus_tag="PC429_001049"
                     /inference="COORDINATES: protein motif:HMM:NF013043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846566.1"
                     /translation="MKIGDKFENLTILDIEQRNSRKYCLCKCGNCGNEKWIRADSLKR
                     IKACGCMKSNTQFKQNDLTGKKFGRLTAIKNTNKKAKSGHYIWICKCACGNEIKTTEN
                     NLTTGRTKSCGCLKKESNIKNAKIALKVHKEKNIIDDTNLSIIKKTEAYPNSKTKIRG
                     VYYNKEKRKYCAQIEFKKTHYNLGYYDNIEDAEKAYIEAKDNLHKKFLEKLHE"
     gene            complement(1807..2256)
                     /locus_tag="PC429_001050"
     CDS             complement(1807..2256)
                     /locus_tag="PC429_001050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846567.1"
                     /translation="MEKFIRLDFDKGFRGKEHRSSATGDGEHFEAGISCYKINKEKCV
                     DAIINLCEYWFEFAGECQFKDFDINIFEGYYVGEGASYEDLATCENHLHCVDGSLFNE
                     VYDLYYMHETYLEENRDIEELEENYKDEYITTEEFETKIKEMFIKYL"
     gene            2409..2618
                     /locus_tag="PC429_001051"
     CDS             2409..2618
                     /locus_tag="PC429_001051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846568.1"
                     /translation="MKDIFEELISIKDACELYKKGESTLRLNIRNKKFVEGIDCKKFG
                     TTWVFKKTALEREYNKKDKKRESGN"
     gene            complement(2645..3484)
                     /locus_tag="PC429_001052"
     CDS             complement(2645..3484)
                     /locus_tag="PC429_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3846569.1"
                     /translation="MKICITVGHSILKSGACTSANGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEKIYKIPRVNSGGYDLLIELHLNSSGVGAFGTEVFYYSE
                     KGKEYAQRVVDKLSKPFIRKKGDKEVGNRGVKLDKSLYILNSSKPTAILIESFFCDNK
                     EDYEKAKKLGHEGIAKLIVEGVLNKNINNEGVKQMYKHTIIYDGEVDKIPATVVGWGY
                     NDGKILICDIKDYVPGQTQNLYVVGGGACEKISSITKEHYTMIKGNDRFDTLHKVLDF
                     IKK"
     gene            complement(3484..3915)
                     /locus_tag="PC429_001053"
     CDS             complement(3484..3915)
                     /locus_tag="PC429_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF3846570.1"
                     /translation="MNVTIVFLATNIFIKLVILAIAFDTLLGCLRAIKTHQFNSSFGI
                     NGGIRKVAMIACIFFLAVVDILTKFNFLFMLPQDWVDFLRLNHLGISEFFSILFILYE
                     SVSILKNMYLCGLPVPKRLKEKIGSLLDTMTDELNVKGGSK"
     gene            complement(3978..4160)
                     /locus_tag="PC429_001054"
     CDS             complement(3978..4160)
                     /locus_tag="PC429_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846571.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFELDDVTNAEYRKAVED
                     YIIEHSGVVEGTQPTK"
     gene            complement(4160..4453)
                     /locus_tag="PC429_001055"
     CDS             complement(4160..4453)
                     /locus_tag="PC429_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846572.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILEHDTITELHYIDVEYGS
                     IDYTRNRIIGINIETKEPILEEIPVFISEEEKRIQELENQILLNENEKVGGIL"
     gene            complement(4470..6161)
                     /locus_tag="PC429_001056"
     CDS             complement(4470..6161)
                     /locus_tag="PC429_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846573.1"
                     /translation="MAIVYEFNYTGAEQSVVLPPGKYKFECFGACGGNYYDFVQCARG
                     GYTAGSLILKENTTLHVYVGQSGYCKGVNGIETCRSGFNGAGGITTYKSTSDGYYSLA
                     GGGATDIRLIGGNWDNLQSLLSRIIVAGGGGGGSGNSHDSIGHGGGTKGKDGISIANK
                     YFAGGGSQFQGGLTFNSLYNGSFGVSGAGDGISGVGGGGGWYCGAGSFYAEFGGGGSG
                     YILTKDSYKPANYSPSSKYYFSDINSVVGGNTTKQDGYAKITLLQALPFLTISSYNST
                     TATFKADHTDTTLLTKIEYFIDDVLKETITTDLTAEKTINYTVEDNALHALKIVVTDS
                     ANATAEKVVSISKGIAPLPAGSTTDEVTSKWMEIKDTFKSGKTSIINTLALKNIEASL
                     NNTLVELSEKIKTSFDSSDASVQDLMNQLTQANNTISQLNSKYKVASGNSVVNYCTNT
                     KGFYFNSDYLFLFPGAIQINGLNFVPNIFFTTFELIDDGYFHKYFVFACRGIFTQDFV
                     ITAHYYRLKSYLQDFKVGGEVLKLNERDVYMDNRGIQLPCSRQGSSFKWQAIKFI"
     gene            complement(6178..7059)
                     /locus_tag="PC429_001057"
     CDS             complement(6178..7059)
                     /locus_tag="PC429_001057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846574.1"
                     /translation="MEKSFVFNSVNGDRRYKAEDFREYFASFIGNGVFPNPSSNLQVV
                     DNNNMTITVKKGKGWINGAIYINTDDLVINIDPADGILNRIDRVVLRFDTLNRNIKLA
                     VKKSAFNSSPAATELQRDADAYELGLADIYIRAGAISITQSSITDLRLDKNLCGIVKG
                     TIEEIDTTTLLAQLSTWKDEEISNFNDWRENQENKQEDWYNTTTTNFVSEFNIWFESI
                     KDILDEDAAGNLLNEINKVKEELAKIETTAEKTSYNNTTSNLTATNVQGAIDEVVRKI
                     EKFNEVNISIQNDMLPI"
     gene            complement(7059..8138)
                     /locus_tag="PC429_001058"
     CDS             complement(7059..8138)
                     /locus_tag="PC429_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus ReqiPepy6 Gp37-like family protein"
                     /protein_id="HDF3846575.1"
                     /translation="MQQSIRILDKNINLIAEIDNYEELQIIKKFHKVGEFSLKINANK
                     NHVDKLVKNNIILLGKNYNKVCLILHREFMYTETGEKSDILSVKGVDLKGLLNRRLII
                     PDTGEAFESHEGTQEEIIKAFVNNNCVDPSNSKRVIDNLIISENKNLGSADMWRSSYE
                     NLSDKIQEISEFCGLGWEVVLDANKKKFIFDVITGKDLTINQTENPPIIFRSDFNNIR
                     ARHFTESIINSKNVVYAGAREDATKLVISTGEVEGFERNEVFADVSENTISILQKEGE
                     IKLKEYEELKSFELEIDPKNTFMYEKDYKLGDIVTVQDRKLKVTMDTRIVEVQESYSK
                     NGIKLKITFGSSIPTLFSKIKRMVK"
     gene            complement(8140..9006)
                     /locus_tag="PC429_001059"
     CDS             complement(8140..9006)
                     /locus_tag="PC429_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HDF3846576.1"
                     /translation="MQKLICRNSKGQEVKLGNSRPFLLEKIENGANTSANISVSNNLG
                     DGVSIDDISIKERLLPISAAIYAKNKEDLELKRAYLTSLFNPKLGEMEFIYTNNALTK
                     RVKGVVQDITFQTPVGLMQKFLIQLLTPSPFWMDEFVLKEEVALWVGDFEFTLEISNE
                     GIEMGHRVSNLICNVVNNGDVECGMKIQFKALATVDNPSLFNVNTREYIKINRTLEAG
                     DLLEITTEFANKRIELVKSNYERINVFNWIDLDSEFLQLEVGDNLFRYDADVGIDNLE
                     MSIYYNPLYLGV"
     gene            complement(9038..14239)
                     /locus_tag="PC429_001060"
     CDS             complement(9038..14239)
                     /locus_tag="PC429_001060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF3846577.1"
                     /translation="MAEDVGELVVRVAMENSSFQQGINNLNRQMKLIRSEFKNSGAGV
                     KNFGTSLDGLKSKQQMLSSTIQQQTKVVEAYKNKLNESKTTLSNTAQKQVELKEKVNQ
                     AKSAYEQSKATLGENAEETKRLKSELDSLEQEYAKNEEKIRSNSAAVDNWSIRVNNAE
                     SRLSEMRSELDDTTERINQQENKWNQLATKMTEIGNKFSSVGKQMQDVGKKMAMSITA
                     PVLAVGAAASKLGMDFEASMSNVQGLSGATADEMVQLEKAAREAGASTSKTAKDAADA
                     LGYMALAGWDAKTSMEALMPVLRLSEAGNLDLARTSDLVTDSMSSLGLSVKELPTYLD
                     QVAKTAASSNTNIDALMEAMIVSGGTFKNLNVPLSEANALLGILANRGLKGSEAGNSL
                     NSIMINLTSGAGQAGVAMKELGLSAFDSNGKFKGMANVLMELKEKTKDMTEEQRTMYL
                     SMIGGKTQISTLQALLSGVGEEYSELEKKVGNSTGALDKMAKTMQNNNKGSITALISA
                     VQELGLKIYDVLKPSIAQVVSWFQNLTNKLNEMKPATVETIVKIAGLAAALGPVLLIG
                     GKIVSMIGGVIGAFGTFSAAMAVATTGAAAATPAVGALAAILGALSSPAAIAVAGITA
                     LGISVATVAKDMSKDAIEPISRFGKSVSESTKEAVGAFMDLEEKTTISLNQLMWSGET
                     VSEKMKQSIVSNFTEMSNQIIAKLQESKEQSIQSLQEMFATSKNLSDKEKEELIKNTE
                     EAYSSKEEKIKKSNEKVNQIMTKASQEHRALTKEESAEINQIKTDMLNTAVDTMSKSE
                     AEQAAIMERMKANHVDLSAKEAAEVVKNSIKKKDESIKAANEEYNERLKIAAQLRAEG
                     GAENEKLADKVVEEATRQKDESINKAKEMHQGVVAEAQKQAENQASKVDWTTGEIKSK
                     WQVLNDTVKGKMQEWDDMAGNWVEGLKEKISTGWANLKENTAILWDNIKLTVTTKWTE
                     IKESIGLKIEEIKQNISEKWESIKLFFTTTWENIKMIFGNAWDAIKQQVEEKFAPFVE
                     RLKIIFDGIKEFFRSTWEVIKNIVLGAVLLILDLVTGNFDKLKTDAIQIWENIKQSLS
                     DAWESIKEVFNNALTAISDLLSNMWEGMKSTASSVWESITQTLSSIWENIKSTCSNAW
                     EGLKTICSNTWTNIKTTLVTSWENMKTAVSTKLTEIKNNIVTVWNSVITFFKTLPAKL
                     KQAAIDMFTRLREGIDSKKQDAVNASQKVGTDVVNKIKEFPSKFLQVGKDFVSGLANG
                     ISGFAYKVVDEAKKLGQKAADAVRKVLGIHSPSRVMAEIGKFVDEGLAQGIANNSDSV
                     KKATEKITKIIEEETKKATKACEEDIKLFNKQIDELSKQEKAALKKVKGDAKDALKEE
                     YAAKKEKIKEAIALRKEQKEKEVEQLKEIANSAKEQLNKELQDRQEFVKKVDDLTKAI
                     VDALKEKYKQEYEAQQASIKKELDALDKWKEESIDRINSVYDAKIKAIDNELEAFEKA
                     EKEKDRLEQDKEELNKIEEIKTAIKFEHDDTNKEQLQKELEKVLAERKKRIEQQEIED
                     KKEALKKRKEELEEKKKDEIENINQIYESEKEYYNKRLEDAKKFYDERIKESKLQAEA
                     EKLIMDKNQKEIVALLNSYSDAYKNAGQTLGEKLLEGFKPAINEIKNLIDSITREINN
                     ARNEALSLNSDIRKGNSAANSNSGRREATYNNNSRTANYNVNVNSTDSNTAREIERTI
                     RSLAFSL"
     gene            complement(14302..14517)
                     /locus_tag="PC429_001061"
     CDS             complement(14302..14517)
                     /locus_tag="PC429_001061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846578.1"
                     /translation="MCKYCEVEESEGAFKRGKTDRGELITCNCDIEMWIEKEGNEYFL
                     NSECWCGYYSESFEPINFCPFCGRTLI"
     gene            complement(14530..14685)
                     /locus_tag="PC429_001062"
     CDS             complement(14530..14685)
                     /locus_tag="PC429_001062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846579.1"
                     /translation="MFLFEKNWSMVDIDNMDIFYYLDILAYMKKNKSDKNNPNENDVY
                     IDQLSWL"
     gene            complement(14769..15056)
                     /locus_tag="PC429_001063"
     CDS             complement(14769..15056)
                     /locus_tag="PC429_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846580.1"
                     /translation="MEIKLTINEEEKIFKAPPIALKKLDNAFALSDKINDGLSMRGLF
                     QELLDFTIDIYGEQFTKEELLNGFYPADEFMSKALEDLSKVSGTFEEKVKN"
     gene            complement(15074..15670)
                     /locus_tag="PC429_001064"
     CDS             complement(15074..15670)
                     /locus_tag="PC429_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF3846581.1"
                     /translation="MAIIGLRNYRIVKLLTDTEETLEYDTKITRLTGAKSVKISPKVD
                     SAENYGDDQLLETASAMGAIEVEIEVADLTLEERALILGYQYQDGVLIEDKNFNPPNL
                     AFGFESPKSQNGSRMVWLTKGVCEPFEEEAKTKEDKIEFQSQKIKLKFMPRINDGRHK
                     ITADTDVENAPTDVEFFTTEFLKTGAKPTAPAKANLNK"
     gene            complement(15674..16009)
                     /locus_tag="PC429_001065"
     CDS             complement(15674..16009)
                     /locus_tag="PC429_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846582.1"
                     /translation="MININNLVIETLKPLKIPVSFQRYNGEEKTYITFFSYLEQNFYA
                     NDEVVGTEHYIQIDLFSKNKMSYINKEIERLLKKNNFIKRSIHEIKESDYSYHTVFRF
                     LFFAKNEED"
     gene            complement(16009..16452)
                     /locus_tag="PC429_001066"
     CDS             complement(16009..16452)
                     /locus_tag="PC429_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF3846583.1"
                     /translation="MGVEITTEGFDAILSKIESMGKSGDKLLNEAVKAGGNVILQDAL
                     PRVSKRSGKLKDGLKVSGVKKKGGTKYVLVGITKEDNSKIFYGKFLEFGASAHQIPIK
                     KGKKKGRVINHPGVSPKPFLAPAYESKKDEAKNVMKEILKRGLGL"
     gene            complement(16453..16779)
                     /locus_tag="PC429_001067"
     CDS             complement(16453..16779)
                     /locus_tag="PC429_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846584.1"
                     /translation="MHSKIVTILKKLNLGIGFQEYDNFSNDEEYIIFFIENEYDANFS
                     DNENKSIVYSIKIEYWYKSLKNINKYISIKDIFKKENFIFLNSSDFIENEYYCKELKF
                     KYEEVV"
     gene            complement(16799..17449)
                     /locus_tag="PC429_001068"
     CDS             complement(16799..17449)
                     /locus_tag="PC429_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HDF3846585.1"
                     /translation="MKKIKFDNFNDGIIEFGEYKESYDIEGNALDEKEFIVNSKLFYS
                     NEYIREQDRLKFDNTGLKVSIKLKVPYMRLIKTSDVIKLNNEFYSIVKLDISNDKTSI
                     YLYLSELVDVLDKHISIFIKERKNVLEDVAMKFYKKIWACVEDLNSKELIENNSIKTS
                     TNKKFKIRYIEELDLSIKQNAITNFVIKYKDKFYNITQILNLEEENKILEITAESK"
     gene            complement(17446..17715)
                     /locus_tag="PC429_001069"
     CDS             complement(17446..17715)
                     /locus_tag="PC429_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846586.1"
                     /translation="MVEEVKEYLNITWIEEDAKIEKMVVRGINYLENEIAGIALDFKD
                     NFNRELLFNYCRYVRNNAIEYFEENFSKELFRLQIKSAANEVTKI"
     gene            complement(17749..18915)
                     /locus_tag="PC429_001070"
     CDS             complement(17749..18915)
                     /locus_tag="PC429_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDF3846587.1"
                     /translation="MAIKNLDEATNVELRTNLMEAIKSNDEEKITTSFLRMAEDIQSN
                     LLKEARSIANIESADRAILDKRGMPQLTSEERAYYSQVIEKRGFTDLEITMPRTIFDR
                     VFDDLEQNHPLLSAITFENTTGVTEWVIRKTGTEAAWWGQLTDPIKKELEGGFAKINT
                     VAYKLSSYLPVAKSMLDLGAEWLDRYVRVVLTESISLGLEMGIVAGTGKDQPIGMMKD
                     LKGSVVEGVYPDKTAKPLADFKPNTLGTEIMAPLTNGGKRTVTSVIMLVNPVDYWAKI
                     FGATTMLTSNGAYVYGVLPIPGNIIQTVAVPKNKLIVGVAKDYFMGIGSAGKIEYSDE
                     YKFLEDVRVYLAKQYATGTPKDNFSFLVFDIENLDTTLATEINSNTKTSATRAK"
     gene            complement(18919..19548)
                     /locus_tag="PC429_001071"
     CDS             complement(18919..19548)
                     /locus_tag="PC429_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HDF3846588.1"
                     /translation="MEKRAVSLEIRDSNLESRKISGYAAIFNDDYTKLQDRWGDTFYE
                     KISRGAFLKTLADNTRDKFMLINHDWNKVIGRTNSNLILEEDERGLRFELDIPATTDG
                     NDLLENVRLGLIKGCSFGFNIVNSKTRFDDDWTYYRDITEVELFEITATPIPAYNDTE
                     INCRSDISIKELRESQKEKVKQNDNKDNIEKRNVDLVSAFFNAFNRGGK"
     gene            complement(19508..20698)
                     /locus_tag="PC429_001072"
     CDS             complement(19508..20698)
                     /locus_tag="PC429_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3846589.1"
                     /translation="MNFKNFISNFLFKDKMSGADGEYFKKLEATIFYKEFALKSCISI
                     IANALVLSEFQTFKNGKSIKEKNYYLFNIEPNQNQNATEFWQEVITRLVYDNECLVIQ
                     ENEQLFVAESFSVDKYVFYEDIYKDVTIRNYKLDRSFRESDVLYFKLNVESIKTVIDY
                     LYNDYKDLLSSAMKGYKKNNSEKGVLEIDTNYPQTKEAQERLNDLMSNKFKRYFESDN
                     AVLPLSNGLKYTENTKASSIKDSRDIRAIIDDVMDFVCAAFHVPAGLVKGNVAGVEGI
                     TDNFLNFSVNPVAKLITNELTRKMYGRDNFFEKSYVKVDTQKIANMGLEKISKASDLL
                     FRIGVNSINDNLEMLGREKINESWADEHYVTKNYQSILNPDLKGGANNGNGEKSGIAG
                     NKGQ"
     gene            complement(20714..22342)
                     /locus_tag="PC429_001073"
     CDS             complement(20714..22342)
                     /locus_tag="PC429_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDF3846590.1"
                     /translation="MILNYKYNKYIDEYFSLADKGEIVLCKEMKLAVKKIKKDLKKPN
                     IYIDHEKVEKAIEYMEKYFYKMYAWEKFVVALVVGCFEGDELLYDEIFIMMGRGNGKN
                     GFISQLANYLQTHLHGVKNYGIDIVAMSEEQAKTSFDDVYNMLSEHPAICKKFFNRTK
                     EVITFKKTNSYLKFRTNNAKSKDGLRPGCIIFDEVHAYENYDNIKVFTSALGKVKNPR
                     IFYITTDGNVRGGVLDDFKEEAKQILNEEIKNSKMLPLIFKLDSIEEMEDVNLLEKAN
                     PSIKYNSDLRTQILKDIDKAKNRPQMMIELLTKRFNLPAEDLATVVAEWDDIVATNEE
                     LPDLKGFSCIGGLDFSSIRDFTSVVLLFKKDKKRYIMHHTFICYKSLEITNFKFDIEL
                     AKQKGLCTIIYDETIKAEYIADWFIEKAEDYNILKICCDSYRAAHVKEEFTNKGLPIE
                     EVRNGSITHAKIAPLVEQIFAERTIKWGDDMMMRWYTNNVYVDTDKKGNKTFLKIEPI
                     KRKTDGFFAFLHALSKDDELMQESTIKFYDCYTY"
     gene            complement(22326..22832)
                     /locus_tag="PC429_001074"
     CDS             complement(22326..22832)
                     /locus_tag="PC429_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HDF3846591.1"
                     /translation="MDDNEELEIRRDVQNDYVLGMTYKEMSAKYNIPENTLKSWRKRG
                     GWTRNCTPKKGCVKNGLQESGNNLKNSIRKDLLRQIKENNMTEEYFKDLVEDYLKMWD
                     IKNELIEDVKTRGVQVKYKNGQNQWGYKKNDSVSELIKYNAQMLSLLDKLGIKATPKV
                     LGDDDIEL"
     gene            complement(23004..23336)
                     /locus_tag="PC429_001075"
     CDS             complement(23004..23336)
                     /locus_tag="PC429_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDF3846592.1"
                     /translation="MSYKHMTLDRLKELILIDRLDIFYRSTDWRKLRKKALERDNNEC
                     QICKEKKKVSKAECVHHIKEVKEYPGEALKLNNLTSLCNLCHNEIHEKILKINQEKKK
                     KFFSEERW"
     gene            complement(23326..23598)
                     /locus_tag="PC429_001076"
     CDS             complement(23326..23598)
                     /locus_tag="PC429_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846593.1"
                     /translation="MNKYGKFKCNNCGKKVNVNNRDLKTKVIDNTTVTYFMCRRCFEK
                     FVVSCEDDYIKHKREEYKKLKDDEVKLLQDMKEYSDGLGELYINEL"
     gene            complement(23771..24319)
                     /locus_tag="PC429_001077"
     CDS             complement(23771..24319)
                     /locus_tag="PC429_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF3846594.1"
                     /translation="MNFVEPIRDLEKLESMCAYLEEANMRNNVLFSMGIYTGLRVGDI
                     LKLRIKDVHDKRFITIKESKTGKTKPIEVNPILKRILKEYTKDKNQNEFLIKSREQTN
                     KAISRVMAYKIIREVGEMFGIDNLGTHTMRKTFGYHYYKRTHDVVTLQKLFNHSSATI
                     TLRYIGIEQDDLNKAYRNIKYF"
     gene            complement(24496..24957)
                     /locus_tag="PC429_001078"
     CDS             complement(24496..24957)
                     /locus_tag="PC429_001078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846595.1"
                     /translation="MKIKKEYIEKVENILKMLGDKYRKLRILKKEIAILKHKENYREI
                     NFEELGFKVKRSTKGIDDIVVTIEDTIYNKETEIEIIERKLEFYNIYLKELSELEQKM
                     IELIYFYNWDEKLPITKIAYELNYDRTSLYNKKAIAINKIALMIYGDEALA"
     gene            complement(25238..25408)
                     /locus_tag="PC429_001079"
     CDS             complement(25238..25408)
                     /locus_tag="PC429_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846596.1"
                     /translation="MGKVEYIVFYYNCETFEVCKKSFSALTDAKVFKNEIIEEYESVD
                     IIKRTVFEELIL"
     gene            complement(25410..25670)
                     /locus_tag="PC429_001080"
     CDS             complement(25410..25670)
                     /locus_tag="PC429_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846597.1"
                     /translation="MNFKMIANIPNWTAKEQFEKVLEEVLELKEAVHLKDNKKIMEEG
                     LDVIQAIFTLFKVLKIDKELSEGLKLHNKKLRKRKWKLEKLK"
     gene            complement(25690..25959)
                     /locus_tag="PC429_001081"
     CDS             complement(25690..25959)
                     /locus_tag="PC429_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846598.1"
                     /translation="MENNFWWSFMDLEEAEKVVTEESIKDLENAINEIENEAEEVKIL
                     IIKKNGDEKYINFKHDGYMKDVIKDRIVQCFEEIKIIHETILEEI"
     gene            complement(25949..26209)
                     /locus_tag="PC429_001082"
     CDS             complement(25949..26209)
                     /locus_tag="PC429_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846599.1"
                     /translation="MKEYRIEIGEMIFLKHKVIVEVPEQICAFDIYKYIENECQNKSE
                     ISEYIEKIGGKQLNFIEGDSGDSDLTVESCKRIKKGGKKNGK"
     gene            complement(26206..26355)
                     /locus_tag="PC429_001083"
     CDS             complement(26206..26355)
                     /locus_tag="PC429_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846600.1"
                     /translation="MSNTNKKTAKICGWCGTTIYSYMDGKIAYCSKKCKKKREKNLET
                     EGAKK"
     gene            complement(26372..26779)
                     /gene="ssb"
                     /locus_tag="PC429_001084"
     CDS             complement(26372..26779)
                     /gene="ssb"
                     /locus_tag="PC429_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3846601.1"
                     /translation="MNSVILIGRLTKDPELKYIPGSGTAVSTFTIAIDRDYIKKDGTK
                     ETDFIPIEVMGKLAEVCANNLGKGRLVAVEGSIRVNSYEKDGEKRTYTKVHANKIKFL
                     DYKKDDTEKEYMFEPKGLEQQGFQAIDDPDIPF"
     gene            complement(26794..26967)
                     /locus_tag="PC429_001085"
     CDS             complement(26794..26967)
                     /locus_tag="PC429_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3797 domain-containing protein"
                     /protein_id="HDF3846602.1"
                     /translation="MKMINVFKLSSMYCICPKCGSDKLGKNEGKLIVDEHTYYRECKC
                     GFNVLVDEREDEI"
     gene            complement(27026..27241)
                     /locus_tag="PC429_001086"
     CDS             complement(27026..27241)
                     /locus_tag="PC429_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846603.1"
                     /translation="MNKISVTQALEKFDSLLDNWNDLPNHVYKKEYRGKFYDWIKSLE
                     RKDSLQNYKIVEVLNNERNGEEAPFWN"
     gene            complement(27234..27410)
                     /locus_tag="PC429_001087"
     CDS             complement(27234..27410)
                     /locus_tag="PC429_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846604.1"
                     /translation="MDKFVTCARCERTINVEENNYVKYEEETLNLSFTLYFCLGCVDK
                     LIEEQLKSMEGENE"
     gene            complement(27430..27795)
                     /locus_tag="PC429_001088"
     CDS             complement(27430..27795)
                     /locus_tag="PC429_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846605.1"
                     /translation="MNIGSYTLVKKQLVKELEEENKQKEECLKEREKHIKELEESLEY
                     FARDALESEECIIDIENILIEQAVELEAYNLFIDCILNKDIRKAIAIYNRTKSIRIKH
                     KNINSISKKIEKIKQIISL"
     gene            complement(27782..27958)
                     /locus_tag="PC429_001089"
     CDS             complement(27782..27958)
                     /locus_tag="PC429_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846606.1"
                     /translation="MQKRITEMSILEKVDLIGQKQKKLQEKYKNLSKGELFSIACNEV
                     KKIEEQEGKYFEYR"
     gene            28047..28391
                     /locus_tag="PC429_001090"
     CDS             28047..28391
                     /locus_tag="PC429_001090"
                     /inference="COORDINATES: protein motif:HMM:NF022172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HDF3846607.1"
                     /translation="MVTNQDCIRDILLLIESNTTPISPVLQFDTILDNLTYDKSTIVH
                     HINLIYQNNLVDDVNYGDDTVLEVMQLSLKGYQYLDKIRDNTKWNKIKSSTSSFLNMS
                     LPIAIEYILGKF"
     gene            complement(28430..28711)
                     /locus_tag="PC429_001091"
     CDS             complement(28430..28711)
                     /locus_tag="PC429_001091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846608.1"
                     /translation="MIKNNVLSYEEKIKVLKKHGYSDFTKEYVLKEFDKEHAFYKSFL
                     RAIEKEDDGKGVLIKKIGKGQHQFSKGFIKTHTISELLEEAKRIRKILE"
     gene            complement(28757..29359)
                     /locus_tag="PC429_001092"
     CDS             complement(28757..29359)
                     /locus_tag="PC429_001092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846609.1"
                     /translation="MEINSVRELRELFELFTVIKAEGNNVVSCNETVNELSNKELIKA
                     NENKFKKPWNDALLYEINALDIIKKHKHSDRKEKAYKTYLGLENADIEEIYKIHYGDK
                     ENSETVASCEEISSAKTDTISCNHDSVSKGKDTSDSECTCFICSKKIENQEDVFKIPF
                     LTRDYLSSAKEIKNIEICEKCLDGNSWSIIRRKQFKKMKK"
     gene            complement(29384..29479)
                     /locus_tag="PC429_001093"
                     /pseudo
     CDS             complement(29384..29479)
                     /locus_tag="PC429_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            complement(29540..29935)
                     /locus_tag="PC429_001094"
                     /pseudo
     CDS             complement(29540..29935)
                     /locus_tag="PC429_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
     gene            complement(30577..30792)
                     /locus_tag="PC429_001095"
     CDS             complement(30577..30792)
                     /locus_tag="PC429_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846610.1"
                     /translation="MNISNFNKYQKGEIVQLYETKFSCNRNCIVTYADEKELVLMYYD
                     KEIEELIYKTLTKEDLIFNDYELKLLS"
     gene            complement(30805..30945)
                     /locus_tag="PC429_001096"
     CDS             complement(30805..30945)
                     /locus_tag="PC429_001096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846611.1"
                     /translation="MVNINEMREEIYRKIELLDPTKLKLALNRVNILKEIQDIEEENI
                     QN"
     gene            31091..31459
                     /locus_tag="PC429_001097"
     CDS             31091..31459
                     /locus_tag="PC429_001097"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3846612.1"
                     /translation="MIDKRIRELRLKLNLSQDEFANELTLKRNTISLIENGKRNTSER
                     TLNDICKKWNVSEEWLKNGTGEMFTIKDLDEQYASLVGELSKKENEVEREIILKMFKL
                     NKKYLTVFDKMIDSLLEIEE"
     gene            31831..32130
                     /locus_tag="PC429_001098"
     CDS             31831..32130
                     /locus_tag="PC429_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3846613.1"
                     /translation="MESLLKYHRKKKKLTQRRLSELTGISQSYISRMENKKFKHSPTL
                     TQIILLADILEIDQLILSEFFLEKEREHLKNTKKYNKKAVKTLSRDFLKEYKNKD"
     gene            complement(32158..32832)
                     /locus_tag="PC429_001099"
     CDS             complement(32158..32832)
                     /locus_tag="PC429_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846614.1"
                     /translation="MMLKYITKHKNMEESRMKKVLLLITVCLLSVGLVACSSTNNKDT
                     GNTTKKEKKANENEVDGIKITVKDVTKEDIKGDINEDGSFNENGEYFSDGIEKKKAAD
                     YVYEVVNVEVENKTDKAVKLFQTGWNAVGTDGYEFKDIKVTDKLDNQQVPANYKFDAQ
                     VKILVEKNMNVKEIVLKYNLKDYSRLFEAMEYANQGASKSDVEKKFPELYKDNWIELG
                     EIKVEQ"
     gene            complement(33471..34163)
                     /locus_tag="PC429_001100"
     CDS             complement(33471..34163)
                     /locus_tag="PC429_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3846615.1"
                     /translation="MSLEEAKELEKNYKLPMPTYCLNEKNYCAKEFGALMLISKGSMR
                     IMHIEKNRYLYQNKFKMEELAKQIGVARTTLERNIKKLNSLDCKVLEIENSRNGIIYR
                     LNYGTSTGYNDNVHKFVTIHHDMLQELISAFNTNAIKVYCLLCYMTTENSFKCMTEKF
                     ICEKIGLCGDSKNNRSKIRKIITVFEVSKYIEVKKENKFEWDEEKNKKVPRIQKLYRL
                     CSFSEWKNARKK"
     gene            35544..35726
                     /locus_tag="PC429_001101"
     CDS             35544..35726
                     /locus_tag="PC429_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF3846616.1"
                     /translation="MKKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
BASE COUNT        10644 a         6493 c         3918 g        14745 t
ORIGIN      
        1 tagttttatg tagaatgtta aaataattta ttaataatat cgatatattg ttgcagcatt
       61 atagaaacaa tactatagtg ttattataaa taaaaaaagg ggttggaaaa gatgaatgtt
      121 tatttgcaaa ttggaaaaga gttatctgaa aaaatgacta aatctgaatt aaataagttt
      181 tcacaagaag taacaaatta tttgcgattc gctgcacttg ataatgatat gaaaattaaa
      241 ctatttcaaa caatcataga agcagttgca agaaaaaaag tatcattacc aaatgacagc
      301 gaatttacag aaggaatatt gcaaagtagc gaattggaaa tgacgacgaa cgtaggattt
      361 ataatcgaag gattaagtta tcataataac taaatcttta gcattaaaaa agcagtgtat
      421 ttattgaata cactgttttt atttccgcat agttaatcta aaataatttg gatattgtat
      481 catttcgata taaattaaat tgatatttaa atcccttata gttaatataa aaattcttat
      541 tacaatatca tttctaaata aattaaaaaa tattttaaat ccctcgtagt taatcttaaa
      601 agcgatacta tatacattaa taataatact ctttcattta aatttcaatc attaatttta
      661 aatcttgttt acaattttct atactttcac ttaactcttt caaaaaaatt ggtaaaccat
      721 cttctatatt tctttcaact aattcgtcaa aattttcttt gttgaacttt tcataactgt
      781 tccaatttaa agtctcgctt tcagtgtcac accaagtagc aaattcttcg taatcattta
      841 aaaagcaaag ttctcctaaa aaatcatcaa tttgaacttt ttcttgacta aattcataaa
      901 caataacttc tctcatatta aaaatatcat ctgaatagcc ttgccaacta gaaattgtgc
      961 tttttacaac tccttccaaa tttatagaaa ttcttatagc atcagttgtt tctattaagt
     1021 agcgttcttt ataaattttc gtagcttctt cctctatttt atctatattc atatctatag
     1081 ctcttagaaa tcttttagcc ttttgtttat tcattttaat ttcctcctct actatcaaat
     1141 atatttatta ttattattca tgcaattttt ctaaaaattt tttatgtaaa ttatctttag
     1201 cttctatata tgccttttca gcatcttcta tgttatcgta gtaacctaga ttataatgtg
     1261 ttttcttaaa ttctatctgt gcgcaatatt ttcttttttc tttattataa taaactcctc
     1321 taattttagt ttttgaatta ggatatgctt ctgtcttttt tataatagat agatttgtat
     1381 catcaataat atttttttct ttatgtactt ttaatgctat ctttgcattt tttatattag
     1441 attccttttt caagcatcca catgatttag ttctaccagt agttaaattg ttttctgttg
     1501 tcttaatctc atttccacaa gcacacttac aaatccaaat ataatgacca cttttagctt
     1561 ttttgttagt gttctttatt gctgttaatc tgccaaactt tttgcctgtt aaatcatttt
     1621 gtttaaattg tgtgttgctc ttcatgcacc cacatgcttt tattcttttc aaactatctg
     1681 ctcttatcca cttttcattt ccacaattcc cgcatttaca tagacaatat tttctactgt
     1741 ttctttgttc tatatctagt attgtaagat tttcaaattt atctccaatt ttcatgtttt
     1801 cacttcctac aggtatttta taaacatttc ttttatctta gtttcaaatt cttctgttgt
     1861 tatatattca tctttgtagt tttcttctaa ttcttcaata tctctatttt cttctaagta
     1921 tgtttcatgc atataatata agtcataaac ttcattaaac aaagaaccat ctacacaatg
     1981 taagtgattt tcacaagttg ctaaatcttc ataactagcc ccttcaccta catagtaccc
     2041 ttcaaaaata tttatatcga aatctttgaa ttgacattca cctgcaaatt caaaccaata
     2101 ttcacataaa tttattatag catcaacaca tttttcttta tttattttat agcaactaat
     2161 tcctgcttca aaatgttctc catctcctgt tgcgctactt ctatgttctt tacctctaaa
     2221 acctttatca aaatcaagtc tgatgaattt ttccattttt taaatccccc ttttatttat
     2281 ctctttcttt aattacatta taaacgctta cgtttattaa gtcaatagtt ttttaaacat
     2341 aaacgtttat ttttttcttg tctttttaaa cttttgcgat tataatataa gtaaaaggag
     2401 ttgttaaaat gaaagatata tttgaagaac taatctctat aaaagatgct tgcgaacttt
     2461 ataaaaaggg tgaaagtacg ttaagactta atataagaaa caaaaaattt gtggaaggta
     2521 tagactgtaa aaaattcgga acgacatggg tttttaaaaa aacagcactt gaaagagaat
     2581 acaacaaaaa ggataaaaaa agagagagtg gtaactgatg aaatagttac cactctttac
     2641 aaacttattt cttaataaaa tctaatactt tgtgaagtgt atcaaatcta tcattacctt
     2701 ttatcattgt ataatgttct ttagttatag aacttatctt ttcacatgct ccaccaccaa
     2761 caacataaag attttgtgtc tggccaggta cataatcttt tatatcacat attaatattt
     2821 tcccatcatt ataaccccat ccaacaacag ttgcagggat tttgtcaact tctccatcat
     2881 aaatgattgt atgtttgtac atctgtttaa ctccctcatt atttatattt ttatttaata
     2941 caccttctac aatcaactta gcaatacctt catgacctag tttcttagct ttctcataat
     3001 cttctttgtt atcgcaaaag aaactttcaa ttagtattgc agtaggttta gaactattta
     3061 aaatatataa acttttatct aatttaacac ctctattacc tacttcttta tctccttttt
     3121 ttcttatgaa aggtttagat aatttatcta ctaccctttg cgcatattcc ttcccttttt
     3181 cactatagta aaatacttct gttccaaagg ctcctacacc acttgaattt aaatgcaact
     3241 ctataagtaa gtcatatcct ccactattaa ctctaggtat tttataaatc ttttcttctg
     3301 ctttagtttt aaactgcttt tcagggcata ttattacatc tgccttatga ccttcttttc
     3361 taaaagtatc tgctaatact ggtgcaagag atttattgta ttgatactcg ttaactactc
     3421 cattagcaga agtacatgcc ccgcttttta aaatgctgtg tcctactgtt atacatattt
     3481 tcattattta ctacctcctt taacatttaa ttcatctgtc atagtatcta ataaactacc
     3541 gattttttct tttaatcgtt taggcactgg taatccacac aaatacatgt tttttaatat
     3601 acttacactt tcatatagaa taaataaaat agagaaaaat tcagatattc caagatgatt
     3661 taatcgcaaa aaatcaaccc aatcttgtgg taacataaat aaaaagttaa actttgtaag
     3721 aatgtcaact actgctagaa aaaatataca tgctatcatt gcaacttttc ttattcctcc
     3781 atttattcca aaacttgaat taaactgatg tgtttttatt gctcttaaac aacctagcaa
     3841 tgtatcaaat gctattgcta atattactaa ttttataaat atatttgttg ctaaaaaaac
     3901 tattgttacg ttcatatttc ctccttattt tgcattaaaa taagacttag aattatctaa
     3961 gcctataaaa aaagaaccta ttttgtaggt tgtgttcctt ctactactcc actatgctct
     4021 ataatataat cctctactgc ttttctgtat tctgcattag ttacatcatc cagttcaaat
     4081 tctcgatttt ttaaagggtt taagcctccg tttaatattc tctctgctaa tattcttact
     4141 acaacattat ttatattcat tataaaattc ctcctacctt ttcattttca tttaataaaa
     4201 tttgattttc taactcttgt attctttttt cttcctccga gataaatact ggtatttctt
     4261 ctaaaattgg ttcttttgtt tctatattta tacctataat tctatttctt gtatagtcta
     4321 tacttccata ctcaacatca atataatgta attctgttat tgtatcatgt tctagtatat
     4381 ctcctgttgc ttctcctgtc tgcaaaagta ttttacctgt ttggtcacat ataattctat
     4441 ttgctctatt cattttatca cctcatttat taaataaatt ttatagcttg ccatttaaaa
     4501 gaagaacctt gtcttgaaca aggaagttgt atacctctat tatccatata aacatcacgt
     4561 tcatttaatt taagaacctc acctccaact ttaaaatctt gtaaatatga ttttagacga
     4621 taataatgag cagtaattac aaaatcttga gtgaaaatac cacgacaagc aaaaacaaaa
     4681 tatttatgaa aataaccatc atcaattaat tcaaaagtag taaaaaatat attaggaaca
     4741 aaattaagtc cgttaatttg aatggctcca ggaaatagga acagatagtc actattaaaa
     4801 taaaatcctt ttgtattagt acaataatta acaacactat tgccactagc aactttatat
     4861 ttagaattta actgacttat agtattatta gcttgtgtta gctgattcat caaatcttgc
     4921 acactagcgt ctgaactatc aaaacttgtt tttattttct ctgacaactc gactaatgtg
     4981 ttatttaaac ttgcttctat attctttagt gccaaagtgt ttataatact tgttttacca
     5041 cttttgaatg tatctttaat ttccatccat ttgcttgtaa cttcatctgt tgtagaacca
     5101 gcaggaagag gtgcaattcc tttgcttata cttacaactt tttctgctgt agcattagca
     5161 ctgtctgtaa caactatttt aagtgcgtgt agtgcattat cttctactgt atagttaatt
     5221 gttttttctg ctgttaaatc tgttgttata gtttctttta gtacatcatc tataaaatat
     5281 tctatcttag tcaataaagt agtgtcagtg tggtcagctt taaatgttgc tgtggttgag
     5341 ttatatgagg atatagttaa aaatggcaat gcttgtagta atgttatttt agcataacca
     5401 tcttgtttcg tagtatttcc acctacaacg ctatttatat cgctaaaata atatttagaa
     5461 gatggactat aatttgctgg cttataacta tcttttgtta gaatatatcc acttccacca
     5521 ccaccaaatt cagcataaaa actacctgca ccacaatacc aaccaccacc gccaccaaca
     5581 ccactaatgc catctccagc accgcttaca ccaaaagaac cattataaag actattaaaa
     5641 gttaaaccgc cttgaaattg gcttccaccg cctgcaaaat atttatttgc tatagaaata
     5701 ccatctttac cttttgtacc accaccatga ccaatactat catgtgaatt tccgcttcca
     5761 ccaccaccac cacctgcaac aattatgcga gatagcaaac tttgtaaatt atcccagtta
     5821 ccaccaataa gtctaatatc agtagctcca ccacctgcaa gactataata accatcagaa
     5881 gtacttttat aagtggttat accacccgca ccattaaaac cacttctaca agtctcaata
     5941 ccattaacac ctttacaata accactttgc ccaacgtaaa catgtaatgt agtattttct
     6001 ttcaatatta aagaaccagc agtatagcca cctcttgcac actgtacaaa atcataataa
     6061 ttaccaccac aagcaccaaa acattcaaac ttatatttac caggtggcaa cactacactt
     6121 tgttcagcac ctgtataatt aaattcataa actatagcca tttttcactc tcctctctta
     6181 aataggtaac atatcgtttt gtatagatat attaacctca ttaaattttt ctatttttct
     6241 aacaacttca tctattgccc cttgaacatt tgtagcagta agattacttg ttgtattatt
     6301 atagcttgtt ttctctgctg ttgtttctat ttttgctaac tcttctttta ctttgtttat
     6361 ttcatttaat aagtttccag ctgcatcctc atccaaaata tcttttatac tttcaaacca
     6421 aatgttaaac tctgatacaa aatttgttgt tgtagtgtta taccaatctt cttgtttatt
     6481 ctcttgattt tcccgccaat cattgaaatt acttatttct tcatctttcc aagtacttag
     6541 ttgtgcaagt aatgttgttg tgtctatttc ttctatcgta ccttttacaa ttccacacaa
     6601 atttttatct aatcttaaat ctgttataga actttgtgta atacttattg ctccagctct
     6661 gatataaata tcagctaatc caagttcgta cgcatctgca tcacgttgta attctgttgc
     6721 agctggggaa ctattaaaag cacttttttt aacagctaat ttaatatttc tatttaaagt
     6781 atcaaatctt aaaactactc tgtctattct atttaaaatt ccatctgcgg ggtctatatt
     6841 aattactaaa tcgtctgtgt ttatatagat agctccgtta atccagccct tgcctttttt
     6901 tactgttata gtcatattat tattatctac aacttgcaaa ttgctacttg gattagggaa
     6961 tacaccatta cctataaaac tagcaaaata ttctctaaag tcctctgcct tgtatctcct
     7021 atcgccattt acactattaa atacaaaact tttttccatt atttcaccat cctttttatt
     7081 tttgaaaata aggttggtat gctgctccca aacgtaattt ttaattttat cccattttta
     7141 ctatagcttt cttgtacttc tacaattcta gtatccattg ttactttcaa ttttctatct
     7201 tgaactgtaa caatatcccc tagtttgtag tctttttcat acataaaagt atttttaggg
     7261 tctatttcta attcaaaact ttttaactct tcatattctt ttaatttaat ttctccttct
     7321 ttttgtagta tgcttatagt attttcactt acatctgcaa agacttcgtt tctctcaaaa
     7381 ccttcaacct caccagttga aattaccagt tttgtcgcgt cttctcttgc tccagcatac
     7441 acaacatttt tagagttaat tatactttct gtaaagtgtc tagctcttat gttattaaaa
     7501 tcgcttctaa aaataatcgg cgggttttct gtttgattaa tagtcaaatc tttaccagtt
     7561 attacatcaa aaataaattt ctttttatta gcatccaaca ctacctccca accaagccca
     7621 caaaattctg atatttcttg aattttatca cttaaatttt cgtaagaact tcgccacata
     7681 tcagcacttc caagattttt gttttctgaa atgataagat tatcaattac acgttttgaa
     7741 tttgaagggt ctacgcagtt attattaaca aaagctttta taatttcttc ttgtgtccct
     7801 tcgtgacttt cgaaagcttc tccagtgtcg ggtatgatga gccttctatt taacaagcct
     7861 tttaaatcca cgcctttaac agataaaatg tcgctttttt ctcctgtttc ggtgtacatg
     7921 aactctcgat gaagtatcaa acaaacttta ttataatttt taccaagtag aattatatta
     7981 tttttaacta atttatctac atgattttta ttagcattta tttttaaact aaactctcct
     8041 actttgtgga attttttaat aatctgtaat tcttcgtaat tatctatttc agctattaaa
     8101 tttatatttt tatctagtat tcttatgctc tgctgcatat tacaccccca aataaagtgg
     8161 attataatat atagacattt ctaaattatc aattcctacg tctgcgtcat atctaaataa
     8221 attgtctcca acttcaagtt gtaaaaattc gctatccaag tcaatccaat taaatacatt
     8281 tattctttca tagtttgatt ttactaactc tattctttta tttgcaaatt ctgttgttat
     8341 ttctaataag tcaccagctt caagggttct gtttatctta atatattctc ttgtgtttac
     8401 attaaatagg ctaggattat caactgttgc aagtgcttta aattgtattt tcatgccaca
     8461 ttcaacgtct ccgttattca ctacattaca aattaaatta cttaccctat gacccatttc
     8521 tattccttca ttacttattt ctaaagtaaa ttcgaagtca ccaacccata aggcgacttc
     8581 ttcttttaaa acaaactcat ccatccaaaa tgggcttgga gttaagagct gtataagaaa
     8641 cttttgcatc aatccaactg gtgtttgaaa tgttatgtct tgcacaactc ctttgacacg
     8701 tttagttaat gcgttatttg tatatataaa ttccatttct cctaatttag gattaaacaa
     8761 agaggttaaa tatgcacgtt ttaattctaa atcttcttta ttttttgcat aaatagctgc
     8821 tgaaatagga agtaatctct cttttatgct tatatcatct atacttacac catcacctag
     8881 attgttcgag actgaaatat tagcactagt atttgctccg ttttctattt tttctaacaa
     8941 aaaagggcgt gagtttccta atttaacttc ttgccctttc gagtttctac atattaattt
     9001 ttgcaagttt cacacccctt ttttataaac tattttttta taaactaaat gctagacttc
     9061 ttattgttct ttcgatttct cttgctgtgt ttgagtctgt actattaacg tttacattat
     9121 aattcgctgt tcttgaatta ttattataag ttgcttctct tcttcccgaa tttgaattag
     9181 ctgctgaatt tccttttcta atatcgctat ttaaactcaa tgcttcattt ctagcattat
     9241 ttatttctct tgtaatacta tcaattagat ttttaatttc gtttatggca ggcttaaatc
     9301 cttcaagtaa tttttcacct agagtttgac ctgcattttt atatgcatct gaataactat
     9361 ttaataacgc aactatttct ttttgatttt tatccattat caatttttca gcttctgctt
     9421 gaagttttga ttcttttatt ctttcatcat agaacttttt agcgtcttca agtcttttat
     9481 tataatattc cttttcgctt tcatagattt gatttatatt ttctatttcg tcttttttct
     9541 tttcttctaa ttcttctttt ctctttttta aagcttcttt tttatcttct atttcttgtt
     9601 gctctattct tttttttctt tcagctaaaa ccttctctag ttctttttgc aactgttctt
     9661 tgttagtatc atcatgttca aactttatag ctgttttaat ttcttctatt ttgtttaatt
     9721 cttctttgtc ttgttcaagt ctatcttttt ccttttcagc tttttcaaaa gcttctaatt
     9781 cgttgtcaat agctttaatt ttcgcatcat agacactatt aattctatct atactttctt
     9841 ccttccattt atctagtgcg tctaactctt tttttatact cgcttgctgt gcttcgtatt
     9901 cttgtttgta tttttctttc agcgcatcaa ctattgcttt tgttaagtca tcaacttttt
     9961 ttacaaactc ttgtctgtct tgtaactctt tatttaactg ttctttagct gaatttgcta
    10021 tctcttttag ctgttcaact tctttttctt tttgttcttt tcttagtgct atcgcttctt
    10081 ttattttttc ttttttagct gcatattctt ctttcaatgc gtctttagcg tcacctttta
    10141 cttttttaag cgctgctttt tcttgtttag ataattcgtc tatttgttta ttaaatagtt
    10201 ttatatcttc ttcgcatgct tttgtagctt tctttgtttc ttcttctata atctttgtta
    10261 tcttttcagt tgcttttttg accgagtcgc tattattagc aatcccttgt gctaaacctt
    10321 catcaacgaa tttaccaatc tcagccataa cacgagatgg agaatgtatt cctaacactt
    10381 ttctaaccgc atcagcagcc ttttgcccta gttttttagc ttcgtctact actttataag
    10441 cgaagccgga tataccattc gctaagccgc ttacaaaatc tttaccaact tgcaggaact
    10501 tgcttgggaa ctcttttatt ttgtttacaa catcagtccc gactttttga gatgcattaa
    10561 cagcatcttg ttttttcgag tctatacctt ctcttagtct tgtaaacata tcaatagcgg
    10621 cttgttttaa tttggctgga agtgttttaa aaaaagtaat aacactattc caaacagtta
    10681 caatattatt tttaatttca gtcaatttag tactaacagc tgttttcatg ttttcccatg
    10741 aggttacaag ggttgttttt atatttgtcc aagtatttga gcaaattgtt tttagccctt
    10801 cccaagcatt agaacatgtt gactttatat tttcccatat actgcttaaa gtttgtgtta
    10861 tagattccca tacgcttgat gcagtacttt tcataccttc ccacatgtta gaaagtaaat
    10921 cgcttattgc agtcaacgca ttgttaaata cttcttttat agattcccac gcatccgata
    10981 agctttgttt tatgttttcc caaatttgta ttgcgtcagt ttttagttta tcaaaattcc
    11041 ctgttactaa atctaaaatt aataacacag caccaagtac tatatttttt ataacttccc
    11101 aagtgcttct aaaaaactct tttataccat caaatataat ttttaacctt tcaacaaaag
    11161 gcgcaaattt ttcttcaact tgttgtttta tagcatccca agcgttcccg aaaatcattt
    11221 ttatattttc ccacgttgtt gtaaagaata attttataga ctcccatttt tcgctgatat
    11281 tttgttttat ttcttctatc tttaagccta tagactcttt tatctctgtc catttagttg
    11341 ttacagtaag ctttatatta tcccacaaaa tagctgtatt ttcctttaaa ttagcccatc
    11401 cagtagatat tttttctttc aagccttcta cccagttgcc cgccatgtcg tcccattctt
    11461 gcatcttacc ttttaccgta tcatttaaaa cttgccactt agattttatt tctcctgttg
    11521 tccagtcgac tttactcgct tggttttctg cttgtttctg tgcttctgca acaactcctt
    11581 gatgcatttc ttttgcttta tttattgatt cgtctttttg tcttgttgct tcttctacta
    11641 ctttatccgc taacttttca ttttctgcgc cgccctctgc tctaagctgt gcggctattt
    11701 ttaatctttc gttatattct tcattagctg cttttatact ttcgtctttt ttctttattg
    11761 agtttttaac tacttccgca gcttctttag ctgacaaatc aacatgattt gctttcattc
    11821 tttccattat tgcagcttgc tctgcttcgc ttttactcat tgtgtcaact gcggtattta
    11881 gcatatctgt ttttatttga tttatctctg ctgattcttc ttttgttaat gctctgtgtt
    11941 cttgactagc tttagtcatt atttgattta ctttttcatt gctctttttt attttttctt
    12001 ctttgcttga atatgcttct tctgtatttt ttattaactc ttctttttct ttgtcactta
    12061 aatttttaga agttgcaaac atttcttgca aagactggat actttgctct ttactctctt
    12121 gaagtttagc aatgatttga tttgacattt ctgtaaaatt agatactatt gattgtttca
    12181 ttttctctga aacagtctca ccactccaca ttaattgatt taaagatatc gtcgtttttt
    12241 cttctaaatc cataaaagcg cctacagctt cttttgtaga ttctgatacg cttttaccaa
    12301 aacggctaat tggctctata gcatcttttg acatatcttt agcaactgtc gcaactgata
    12361 tgcctagcgc agtaattcca gctacagcta tagccgcagg gcttgataat gcacctaaaa
    12421 ttgcagctaa agcaccaaca gctggagttg ctgcggctgc tccggttgtt gcgactgcca
    12481 ttgccgctga aaaagtacca aaagccccta taactccacc tatcatgcta actatttttc
    12541 caccaataag taaaacgggt ccgagtgctg ctgctaatcc tgctatttta actatagttt
    12601 ctactgtagc aggtttcatt tcgtttaatt tatttgtaag gttttgaaac cagctaacta
    12661 cttgcgctat agaaggtttt agtacatcat atattttaag tcctaattcc tgtacagctg
    12721 atattaaagc cgttatagaa cctttattat tattttgcat agtcttagcc attttatcta
    12781 atgcacctgt gctatttcca accttttttt caagttctga atattcttct ccaactccag
    12841 atagcaaagc ttgcaaagta gatatttgcg tttttccacc tatcattgac aaatacattg
    12901 ttcgttgttc ttctgtcatg tcttttgttt tttctttaag ttccatcaaa acattagcca
    12961 tgcctttgaa cttaccattg ctatcaaaag cacttaatcc tagttctttc atagctacac
    13021 cagcttgacc tgctccactt gtcagattta tcatgataga gtttaaagaa tttcctgcct
    13081 cgctgccttt tagtcctcta tttgcgagta ttccaagtag tgcgttagct tccgaaagag
    13141 gtacgtttaa atttttaaaa gtacctccag acacaatcat agcttccata agtgcgtcaa
    13201 tgttagtgtt tgaacttgca gcagttttgg caacttggtc taaatatgtt ggcaattctt
    13261 ttacactaag cccgagactt gacatagagt cagttactaa atccgaggtt cttgcaaggt
    13321 ctaaatttcc agcttccgac aatctcaaaa caggcatcaa agcttccatt gatgttttcg
    13381 catcccagcc ggccaaagcc atatacccaa gcgcatcagc cgcgtcttta gctgttttgg
    13441 atgtagatgc tcctgcttct ctagctgctt tttcaagctg aaccatttcg tcagctgttg
    13501 ccccgcttaa tccttgtaca tttgacatag atgcttcaaa atccattcca agcttactag
    13561 ccgctgctcc gactgctaaa acaggagcag taatactcat agccattttt ttacctacgt
    13621 cttgcatttg ttttccaacc gagctaaatt tgttacctat ttcagtcatt ttagttgcta
    13681 actgattcca tttattttct tgttgattta ttctttctgt tgtgtcatct agttctgaac
    13741 gcatttcaga aagtctcgac tctgcattat taactcgaat agaccaatta tcaaccgctg
    13801 ccgagttaga ccttattttt tcttcatttt tagcatattc ttgttctaaa ctatcaagtt
    13861 cagattttag cctttttgtt tcttctgcgt tttcacctaa tgtcgctttg ctctgttcgt
    13921 aagcactctt agcttgatta actttttctt taagttcaac ttgcttttgt gccgtgttgc
    13981 ttaaagttgt tttactttca tttagtttat ttttatatgc ttcaactact ttagtttgct
    14041 gttgtatagt actagataac atttgttgtt tagatttcaa accatcaaga ctagttccga
    14101 aatttttcac tccagcgcca gaatttttaa actcacttct tattaacttc atttgtctat
    14161 tcaagttatt aatcccttgt tgaaacgagc tgttttccat tgcaacacga acaactaatt
    14221 cgcctacatc ttccgccatt tcctcacctc acttgcaaaa aaataaacca agatatttta
    14281 tcttggttta tttcaaaatt attaaattaa tgttcttcca caaaaagggc aaaagttaat
    14341 aggttcaaaa ctttcactat agtaaccaca ccaacattca gaatttaaaa aatactcatt
    14401 accttctttt tcaatccaca tttctatatc acaattacaa gttattaatt cccctctgtc
    14461 tgttttacct cttttaaaag ctccttcgct ttcttcgact tcacaatact tacacataca
    14521 ctcacatcct tataaccagc ttaattggtc tatatagaca tcattttcat tcggattatt
    14581 cttatcgctt ttatttttct tcatatacgc tagtatatct aaataataaa atatatccat
    14641 attgtctata tcgaccatgc tccaattctt ttcaaataaa aacatactat ataaatcttt
    14701 tataaagtcc cgtggagata gatacttatt ttctttatta gaattatttt ctgtaccttc
    14761 tccatcactt agttttttac tttttcttca aaggttccag atacttttga caaatcttct
    14821 aatgcttttg acataaattc gtcagcagga taaaacccat ttagcaattc ttcttttgta
    14881 aattgttcgc catatatatc tattgtaaaa tctaataatt cttgaaataa ccctctcata
    14941 ctaagcccat cattaatttt atcagataat gcaaaggcat tatctaattt tttaagtgct
    15001 ataggtggag ctttaaatat tttttcttct tcatttatag ttaattttat ttccattttt
    15061 tattttctcc tttttattta tttaaatttg cttttgctgg tgcagttggt tttgctcctg
    15121 tttttaaaaa ttctgttgtg aaaaactcta catctgttgg ggcgttttca acatctgtat
    15181 ctgctgttat tttgtgtcta ccatcattta tgcgcggcat aaatttaagt ttaatctttt
    15241 gagattgaaa ttcgatttta tcctcttttg tttttgcttc ttcttcgaaa ggttcacaga
    15301 cacctttagt tagccaaacc attctacttc cgttttggct ctttggggat tcaaaaccaa
    15361 aagcaaggtt tggaggattg aaatttttat cctctatcaa cacaccatct tgatattggt
    15421 atccaagaat aagcgcacgc tcttctaatg tcaaatcagc aacttcgatt tcaacttcga
    15481 ttgctcccat agccgaagca gtctctaaaa gctggtcgtc tccataattt tctgctgagt
    15541 caactttagg tgatattttc acacttttag cacctgttaa tcttgttatt ttagtatcgt
    15601 attcaagtgt ttcctctgta tctgttagta gctttacaat tctatagttt cttaatccta
    15661 taatagccat ttcttaatcc tcctcgtttt tcgcaaaaaa taagaatcta aaaacagtat
    15721 ggtaactata atcagattct tttatttcgt gtatacttct ttttataaaa ttattttttt
    15781 taagcagtct ttcaatttct ttatttatat aactcatttt attcttacta aacaaatcta
    15841 tttgtatata atgttctgtg cctaccactt catcatttgc ataaaaattt tgttctaaat
    15901 aactaaagaa agttatatat gttttttctt ctccattata cctttgaaat gaaacaggta
    15961 tttttaaagg ttttaatgtt tctattacta aattatttat attaatcatt ataaccccaa
    16021 tcctcttttt aatatttctt tcattacatt tttagcttca tctttttttg actcgtacgc
    16081 aggtgctaaa aacggtttag gactaacgcc tggatgattt attactctac cttttttctt
    16141 accttttttg attggtattt gatgcgcgga tgctccaaat tctaaaaact ttccataaaa
    16201 aatttttgaa ttatcctctt tcgtaatacc aactaaaaca tacttagtac cacctttttt
    16261 cttaactcca cttactttta aaccatcttt taactttccg cttcttttgc tgacccttgg
    16321 taatgcatct tgcaaaataa cattaccgcc agcttttacg gcttcattta gcagtttatc
    16381 gcccgattta cccatactct ctattttaga taaaatagcg tcaaaacctt ctgttgttat
    16441 ctctacaccc atttaaacaa cttcctcata tttgaatttt aattctttac aataatattc
    16501 attttctata aaatcactag aatttaaaaa gataaaattt tcttttttaa atatatcttt
    16561 tatactaata tatttgttta tattctttaa gctcttatac caatattcta tttttattga
    16621 atatacaata cttttatttt cattatcaga aaaatttgca tcatattcat tttctataaa
    16681 aaatattata tattcttcat catttgaaaa attgtcgtat tcttgaaagc caatacctag
    16741 atttaatttt ttaagtatag taactatttt tgagtgcata ctatctccta actttttttt
    16801 acttagattc tgctgttatt tcaagtattt tattttcttc ttctaaattt aagatttgag
    16861 ttatattata aaatttatct ttgtatttaa taacaaaatt agttatagca ttttgtttta
    16921 tacttaaatc taattcttca atatatctaa ttttaaactt tttatttgta gaagttttaa
    16981 tcgaattatt ttctatcaat tctttactat ttaaatcttc aacacaagcc caaatttttt
    17041 tataaaattt cattgctaca tcttcaagaa cattttttct ctctttgata aaaatagaaa
    17101 tatgcttatc tagtacgtca actaattcag ataaatataa atatatacta gttttgtcat
    17161 tactaatatc aagttttact atactgtaaa attcattatt taactttata acatcacttg
    17221 ttttaattaa tctcatataa ggtactttta acttaataga tacttttaac cctgtattat
    17281 caaatttgag tctgtcctgc tctcttatat attcattaga ataaaagagt ttactattta
    17341 caataaactc tttttcatct agtgcattac cttctatatc gtagctttct ttatattctc
    17401 caaattcaat aataccatca ttgaaattat caaattttat ttttttcata tctttgttac
    17461 ctcattagct gcgcttttaa tttgaagtct aaaaagctct ttactaaaat tttcttcgaa
    17521 atattcaata gcattgtttc ttacatatct gcaatagtta aataacaact ctctattaaa
    17581 attatcttta aaatctaatg ctattccggc tatttcattt tctaagtaat taatccctct
    17641 tacaaccatt ttttctattt ttgcatcttc ttctatccaa gttatattaa gatattcttt
    17701 tacttcttca actatacttt taatattttc attttccata tttttcacct attttgctct
    17761 agtagctgac gtttttgtgt ttgaattaat ttctgttgct aaagttgtgt ctaagttttc
    17821 tatatcaaat actaaaaaag aaaaattatc ctttggtgtt ccagttgcgt actgcttagc
    17881 aagatagact cttacatctt ctaagaactt atattcatct gaatactcta ttttaccagc
    17941 tgacccaatt cccataaaat aatctttagc aactcctacg attaatttat tttttggaac
    18001 agcaacagtc tgaattatat ttcctggtat aggcagaact ccatatacat aagcgccatt
    18061 tgaagttagc atagtcgttg caccgaagat tttagcccaa taatcaaccg gatttacaag
    18121 cattataacg cttgtgactg tccttttacc accatttgta agtggtgcca tgatttcagt
    18181 tcctaatgta tttggtttaa aatcagctaa tggttttgcg gttttatctg gataaacacc
    18241 ttcaacaaca gagcctttta agtccttcat catcccaata ggttggtctt ttcctgttcc
    18301 tgctactatt cccatttcaa gccccaatga tatagattca gtcaaaacta ctcttacata
    18361 tctgtctaac cattcagcac ctaggtcaag catagatttt gcaactggaa gataagaaga
    18421 taatttataa gcaacagtat taatttttgc aaatccacct tctaactctt ttttaatagg
    18481 gtcagttaat tgcccccacc aagctgcctc cgttcccgtt tttcttatta cccactccgt
    18541 aacgcctgtc gtattttcaa aagttattgc actaagtagg gggtggtttt gttctaagtc
    18601 atcaaacact ctatcaaata tagtgcgcgg catagttatc tctaaatcag taaatcctct
    18661 tttttctatc acttggctat aatatgctct ttcctcgctt gttaattgtg gcatacccct
    18721 cttgtcaagt atcgccctgt cagctgattc tatattagca atagaacgtg cttcttttag
    18781 taaattactt tgtatatctt ccgccattct taaaaatgat gttgttattt tttcttcgtc
    18841 attactcttt atcgcttcca ttaaattcgt tcttagttca acattagttg cctcatctaa
    18901 atttttaatt gccatttttt attttcctcc tcgattaaat gcattaaaaa aagccgatac
    18961 taagtcgaca ttcctttttt ctatattatc tttattatca ttttgtttta ctttttcttt
    19021 ttgagattct ctaagttctt ttatagatat atcacttcta caatttattt cagtatcatt
    19081 ataagcagga ataggggttg ctgtaatttc aaatagttca acttctgtta tatctctgta
    19141 atatgtccaa tcatcatcaa agcgagtttt tgaatttaca atattaaaac caaaagaaca
    19201 ccctttaatt agtccaagtc ttacattttc aagcaaatca ttcccgtccg tcgtagctgg
    19261 tatatctagt tcaaacctta acccacgttc atcttcttct aaaattagat ttgagttagt
    19321 tctacctata actttattcc agtcatgatt tataagcata aacttatccc tagtgttatc
    19381 tgctaaagtt tttaaaaatg cacctcttga aatcttttcg taaaaagtat cgccccatct
    19441 gtcttgcaat ttagtataat catcattaaa aatagcagca taaccactta tcttacgact
    19501 ttctaaatta ctgtccctta tttccagcga taccgctctt ttctccattt ccattgttag
    19561 cccctccttt caaatccgga tttaaaatcg actgatagtt tttagttaca tagtgttcat
    19621 cagcccaaga ctcgtttatt ttttctctgc caagcatttc taaattgtca tttatagaat
    19681 ttacacctat tctaaataat aggtcgcttg ctttcgatat tttttcaagt cccatattag
    19741 caattttttg tgtatctact tttacataac tcttttcaaa aaaattatct ctaccataca
    19801 tttttctagt taattcattt gtaataagtt ttgctactgg attgacagaa aaattcaaga
    19861 aattatctgt tataccttca actccagcaa cattaccttt tacaagtcca gcaggaacat
    19921 gaaaagcagc gcatacaaaa tccatgacgt catcaataat cgctcttata tccctgctat
    19981 cttttatact tgaagctttt gtattttctg tatattttaa tccatttgaa agcggtaaaa
    20041 cagcattatc actttcaaaa taacgtttaa acttattact catcaaatcg ttaagtcgtt
    20101 cctgcgcttc ttttgtctgc gggtaatttg tgtctatttc aagtacgccc ttttcgctat
    20161 tatttttttt ataacccttc atggcactac ttaataaatc tttataatca ttataaagat
    20221 aatctattac agtttttata ctttcaacat ttaatttgaa atataaaaca tctgactctc
    20281 taaaacttct atctaattta tagtttctaa ttgtaacatc tttgtatata tcttcataaa
    20341 aaacatactt atctacacta aaactttcag ctacgaaaag ttgttcgttt tcttgtataa
    20401 ctagacattc attgtcatac acaagtcttg taattacttc ttgccaaaat tctgttgcgt
    20461 tttggttttg gttaggttct atattaaaca agtaataatt tttttcttta atagattttc
    20521 catttttaaa tgtctgaaat tcacttaaaa ctaaagcatt tgcaataata gaaatacatg
    20581 actttaacgc aaattcttta taaaatatcg tagcttctag ttttttaaaa tattctccat
    20641 ctgctcctga cattttatct ttaaataaaa aattacttat aaaattttta aaattcaatt
    20701 caccacctca ttattaatac gtataacaat cataaaactt aattgttgat tcttgcatca
    20761 gttcatcatc ttttgataaa gcatgtaaaa acgcaaaaaa gccatcagtt ttccgcttga
    20821 ttggctctat ctttaaaaaa gttttatttc cttttttgtc tgtatctaca taaacattat
    20881 tagtatacca acgcatcatc atgtcgtcgc cccacttaat tgtcctctct gcgaagattt
    20941 gttctacaag aggggctatt tttgcgtgag ttatcgaccc atttcgaact tcttcaatcg
    21001 gtaaaccttt gttagtaaac tcttctttta catgtgcagc tctataagag tcacaacaga
    21061 tttttaatat attataatct tctgcttttt caatgaacca atcagcaata tattccgctt
    21121 ttatcgtttc atcataaatt attgtgcaga gacctttttg ctttgctaat tcaatatcaa
    21181 atttaaaatt tgttatttct aaagacttat aacatataaa agtgtgatgc atgatatatc
    21241 gttttttatc ttttttaaat aacaatacca cgcttgtaaa atctcttata ctagaaaaat
    21301 ctaatcctcc aatgcacgaa aagcctttta agtctggtaa ttcttcgttt gttgcaacta
    21361 tgtcatccca ttcagcgaca acagttgcca aatcttcagc tggcaaatta aatcttttag
    21421 ttagtagctc tatcatcatt tgtggtctat ttttagcttt gtcaatgtct tttaaaattt
    21481 gtgtccttaa gtctgaatta tactttatac ttgggtttgc cttttctagc aaattaacat
    21541 cttccatttc ttctatacta tcaagtttaa aaataagtgg cagcatttta gagtttttaa
    21601 tttcttcatt taaaatttgt ttagcttctt ctttgaaatc gtctaatact ccacctctta
    21661 cattcccatc agttgtaata taaaaaattc taggattttt tacttttcca agcgcagagg
    21721 taaagacttt tatattatca taattttcgt atgcatgaac ctcatcaaaa ataatgcagc
    21781 cgggtcttaa tccatccttt gacttagcat tatttgtgcg gaactttaaa tagctgttag
    21841 tttttttaaa tgttattact tctttagttc tgttaaaaaa ctttttacat atagcaggat
    21901 gttcagataa catgttataa acatcatcaa aacttgtttt tgcttgttcc tctgacattg
    21961 caacaatgtc aataccataa ttttttacac catgcaagtg cgtttgtaaa taattagcta
    22021 attgggatat aaaaccattt ttcccatttc ccctacccat catgataaaa atttcatcat
    22081 ataaaagttc gtcgccttca aagcagccaa ccactaaagc aacaacaaat ttttcccaag
    22141 catacatttt atagaaatac ttttccatat attctatagc tttttcaact ttttcatggt
    22201 cgatatagat attaggtttt tttaaatctt tttttatttt cttaactgca agtttcattt
    22261 ctttacatag aacaatttcg cctttatcag ccaagctgaa atactcatct atgtatttat
    22321 tgtacttata attcaatatc atcatcaccc aaaacttttg gcgtcgcttt tatacctagt
    22381 ttatcaagta aacttaacat ttgagcgtta tacttgataa gctctgatac actatcgttt
    22441 tttttatagc cccattgatt ttgaccattt ttgtatttaa cttgaacacc tctagttttt
    22501 acatcttcta ttaattcatt ttttatatcc cacattttta aatagtcttc aactaagtct
    22561 ttgaagtatt cttctgtcat gttattctct tttatttgtc ttaataaatc ttttcttata
    22621 gagtttttta aattatttcc cgactcttgc aacccatttt tcacgcaacc cttttttggg
    22681 gtgcagttgc gagtccaacc ccctcttttt ctccaacttt tcaaagtgtt ttcgggtatg
    22741 ttatacttgg ctgacatttc tttataagtc atacctaaaa cgtagtcatt ctgtacgtct
    22801 cgcctaattt ctaattcctc attatcatcc attttcacca cctcattttt atgttttttc
    22861 tgcacccgca accatgaatt ttggttgcaa gacccccacc ccccacacct tttgttaaat
    22921 tggtgtctcc ttgcggagcc atgtacctac ctcggttcac aaaattttgc cttgaaagcc
    22981 agttttttca ataggggggc tatctaccat cgctcttcac tgaaaaattt tttctttttt
    23041 tcttgattaa tttttagtat tttttcgtga atttcgttgt gacaaaggtt gcacaagctt
    23101 gttaggttat taagcttcaa agcttcacct ggatactctt ttacctcttt gatatgatgt
    23161 acacattccg ccttagatac tttctttttt tctttacaaa tctgacattc attattatct
    23221 ctttcaagtg ctttttttct taatttacgc cagtctgtac ttctatagaa tatatcaagt
    23281 ctatcaatta aaattaattc ttttaatcta tctagtgtca tgtgcttata actcattaat
    23341 atacagctct cctagaccgt cgctatactc tttcatgtct tgcaatagct tgacttcatc
    23401 atctttaagc ttcttatatt cttctctctt atgtttaata taatcatcct cacatgatac
    23461 aacaaacttc tcaaagcatc tgcgacacat gaagtatgta acagtagtat tatcaataac
    23521 tttagtcttt aaatctctat tatttacatt aactttcttt ccgcaattat tacatttaaa
    23581 cttaccatac ttattcatag tgatactcct ttaatttcct agttgagttt aacataagat
    23641 atattctgtt aaactcattc aagtattatt aaacagcctt acattttagt actaacaata
    23701 cattcaagac tttattatta gactatattt tatccagttt aacgtgacct ttttatgtta
    23761 aattcttaag ctaaaagtat ttaatattcc tatatgcttt gtttaaatca tcctgttcaa
    23821 ttccaatata acgcaaggtt atcgtcgctg aactatgatt aaaaagcttc tgtaaggtca
    23881 caacatcatg agtcctttta tagtaatgat aaccaaaagt tttacgcatt gtatgtgtgc
    23941 ctagattatc tatgccaaac atttcgccta cttctcgtat tatcttataa gccattactc
    24001 tacttatagc tttattagtc tgttctctac tcttaattaa aaactcattc tgattcttat
    24061 cttttgtata ttcttttagt attcttttta gaataggatt aacttcaata ggcttggttt
    24121 tacctgtctt actttcttta atagttataa accgtttgtc atgaacatct tttattctaa
    24181 gctttaatat gtcacctact ctaagtcctg tatatatgcc catactaaag agcacattgt
    24241 ttctcatgtt agcttcttct aaataagcac acatgctttc tagcttctct aaatctcgaa
    24301 taggttctac aaagttcatt tactcacccc cttccttttc ggcaataaaa aaagtctaaa
    24361 cttcgagcca agtttagact ttaggtttaa ggggatattc agttgttaat gtacactttt
    24421 ttctatgata caattctaac atgctttctt tagaaatgaa gtataaaaat agtctttttt
    24481 ttgtaaaaat attgtttacg ctaaagcttc atcaccgtat atcattagag ctattttatt
    24541 aatagcaata gcttttttgt tataaagact agttctatca taatttaatt cataagctat
    24601 ttttgtgata ggtagttttt catcccaatt ataaaaatat attaattcta tcatcttctg
    24661 ttcaagctct gataattctt ttaagtaaat attataaaac tctagctttc gctctataat
    24721 ttcaatttca gtttctttgt tgtagatagt atcctctatt gttacaacta tatcatctat
    24781 acctttcgta cttcttttaa ctttaaatcc cagttcttcg aaattaattt ctctatagtt
    24841 ttctttgtgt ttaagaattg ctatttcttt ttttaatatt cttaactttc tatatttatc
    24901 tcctaacatt tttaatatat tttccacttt ttcaatatac tcttttttta ttttcatttt
    24961 tgcacctctt gcactttttg ttataaatac ctaactaatc cttaaattaa ggtttgggga
    25021 ctagttaggc aatttgtagc atttccaact actgcatatt attcaaaata ttctggattt
    25081 ttattttgtt tttttgttat aagacttcat ttttttaagt ttaaacaaaa tctatttata
    25141 taaatacttt tatgaataat gctttaataa ttaatgtaaa tccaaattat ttatctttta
    25201 cactttaatt tatatttttg ccgtttttga atgttaatta caaaatcaat tcttcaaaaa
    25261 ctgttctttt tataatatca acactttcat attcttctat tatttcattt ttaaaaactt
    25321 tagcatctgt taaagcacta aaactttttt tacacacttc gaatgtttcg caattataat
    25381 aaaaaacaat atattcaact tttcccatgt tactttaatt tctccagttt ccattttctt
    25441 tttcttaact ttttgttatg taacttcaat ccctctgata gctctttatc aattttcaaa
    25501 actttaaata gtgtaaaaat tgcttgaata acatctaaac cttcctccat tattttctta
    25561 ttatctttta gatgaacagc ttctttaagt tctaaaactt cttcaagtac tttttcaaat
    25621 tgttcttttg ctgtccaatt tggtatattt gcaatcattt taaaattcat cttttatttt
    25681 ctcctttttt tatatttctt ctaaaattgt ttcatgtatt atttttattt cttcaaaaca
    25741 ttgaactatt ctatctttta taacgtcttt catatatcca tcatgtttga aattaatata
    25801 tttttcatcg ccatttttct taataattaa aattttaact tcttccgctt cattctcaat
    25861 ttcatttata gcattttcta agtcttttat actttcttct gtaacaactt tttccgcttc
    25921 ttctaaatcc ataaaactcc accaaaaatt attttccatt cttttttcct ccttttttta
    25981 ttctcttaca actttcaact gttaagtcgc tatctccgct gtcaccttct ataaaattta
    26041 attgtttgcc accaattttt tctatatact cagatatttc acttttgttt tggcattcat
    26101 tttctatata tttatatata tcaaaagcgc atatttgttc tggaacttca acaataactt
    26161 tatgttttaa aaatatcatt tctccaattt caattctata ctctttcatt tttttgcccc
    26221 ttctgtttct aaattttttt ctcttttttt cttacatttc ttagaacaat atgcaatttt
    26281 accatccata taagaataaa ttgttgttcc acaccatccg caaattttag ctgttttttt
    26341 atttgtatta gacattgtta tccctcccac tttaaaatgg tatatctggg tcatctatag
    26401 cttgaaatcc ttgttgctct aaacctttcg gttcaaacat atattctttt tcagtatcat
    26461 cttttttata gtctaaaaac ttaatcttat tagcatgaac ttttgtataa gttcttttct
    26521 ccccgtcttt ttcataacta ttaactctta tagacccttc aaccgctact aacctacctt
    26581 tacctaaatt attagcacat acttctgcta acttccccat cacctcaata ggtataaaat
    26641 cagtttcttt agttccatct tttttgatat aatctctatc tattgctatt gtaaaagtag
    26701 atacagctgt tccagaaccc ggtatatatt taagttctgg gtcttttgtt aatcttccaa
    26761 ttaaaataac actattcatt tccagcacct tccttatatt tcgtcttctc tttcatctac
    26821 aagaacatta aaaccgcatt tgcattccct gtaataagta tgttcatcaa caattaactt
    26881 tccttcattt tttccaagtt tatcgctccc gcatttgggg caaatacaat acattgatga
    26941 taatttaaaa acatttatca ttttcatatt tatatacatc ccttctatct aacaaaagtt
    27001 taagtccttt tcaagttata tttcttcaat tccaaaacgg cgcttcttct ccatttcgtt
    27061 cattatttaa aacttcaact atcttataat tttgtaaact atcttttctt tcaagtgact
    27121 ttatccagtc ataaaattta cctctatatt cttttttgta aacatgattc ggaaggtcgt
    27181 tccaattatc tagtaaacta tcaaattttt ctaatgcttg tgtaacacta atcttattca
    27241 ttttctccct ccattgattt taattgttct tcaattaatt tatctacgca gcctaaacag
    27301 aaatacaacg taaaacttaa atttaaagtt tcttcttcat acttcacata attattttct
    27361 tctacattta ttgttctttc gcatctagcg catgttacga acttatccat tattaaacct
    27421 ctcctatttc tacaaggaaa ttatttgttt tattttttca attttcttag atatagaatt
    27481 aatattttta tgcttaattc tgatgctttt agttctgtta taaatcgcaa tagcttttct
    27541 tatatctttg tttaaaatgc aatctatgaa taaattataa gcttctaatt ctacagcttg
    27601 ttctatcaaa atattttcaa tatctattat gcattcttct gattctaacg catccctcgc
    27661 aaaatattct aagctctctt ctaattcttt tatgtgtttt tcacgttctt ttaaacattc
    27721 ttctttttgt ttattttctt cttctaactc tttaactagt tgtttcttaa ctaatgtata
    27781 actacctata ttcaaaatat tttccctcct gctcttctat ttttttaact tcattacaag
    27841 caatagaaaa caattctcct ttacttagat ttttatattt ttcttgtagt ttcttttgtt
    27901 tctgtccaat taaatcaact ttttctaata tactcatttc tgtaattctc ttttgcattt
    27961 gctttgctcc ttctcgttct gttacagtaa aatttattat tgtctcgttt ctcctgtgct
    28021 atactaaagc aaaaggaggt gatactatgg ttactaacca agattgtata agagatattt
    28081 tattacttat tgaatctaat acaactccca tttcgcctgt attacaattt gatactatac
    28141 tagataatct cacatacgat aagtcaacta ttgttcatca cataaattta atttatcaaa
    28201 ataatctcgt tgacgatgtt aattacggtg atgatactgt tttagaggtc atgcagttgt
    28261 ctttaaaagg ttatcaatat ttagataaaa tacgagataa cactaaatgg aataaaataa
    28321 aaagttccac ttcaagtttt ttaaatatgt ccctacctat cgcaatagag tacatattag
    28381 gtaaatttta atttaagtat aaggtttaga ttatagacat ttctaagcct tattctaata
    28441 tttttctaat acgttttgct tcttccaata actcactgat agtgtgtgtt tttataaaac
    28501 cttttgaaaa ttgatgctga ccttttccaa tttttttaat taacacacct tttccatcat
    28561 cttctttttc tattgctctt aaaaatgatt tgtaaaatgc atgttccttg tcaaattctt
    28621 ttaaaacata ttctttggta aaatcactat aaccatgttt ttttaatact tttatttttt
    28681 cttcataact taaaacatta ttttttatca ttttttcact ctcctttttc acatgtaaag
    28741 atactaattt aaatatttat ttcttcattt tcttaaactg ctttcttctt ataatactcc
    28801 acgaattacc atccaaacat ttctcacaaa tttcaatatt ttttatttct tttgctgaac
    28861 tcaaataatc tcgtgttaaa aaaggtattt taaatacatc ttcttgattt tctatttttt
    28921 tagaacaaat aaaacaagta cattctgaat cactagtatc tttacctttt gaaacactgt
    28981 catgattgca agatattgta tccgtttttg ctgaactaat ttcttcgcaa cttgcaacag
    29041 tttcagaatt ttctttatct ccataatgaa ttttataaat ttcttctata tctgcatttt
    29101 ctaatcctag ataagtttta tatgcctttt cttttctatc actgtgctta tgctttttta
    29161 ttatatctaa agcatttatt tcatataata gagcatcatt ccaaggcttt ttaaatttgt
    29221 tttcatttgc ttttattagc tctttgttac ttaactcgtt tacagtttca ttgcaagaaa
    29281 caacattgtt gccttccgct tttataactg taaaaagttc aaacaattct cttaactctc
    29341 ttacgctatt aatttccatt tttttacccc acctttttta attttattta aatttagcac
    29401 tttgactttt ctttataatt gcatcaagtt catcttctga atatttatca aatgtttgat
    29461 tgaaattagc aaatttatta ttatttgtct ttgtttttcc tttgacgctc tcattgcctt
    29521 ttagagactt tttaggtgtc tcgtttaagt aagactcgaa tttgtttcca aacaacgtct
    29581 cagggcgtaa ataagcgttc attttatcat catctagcca atttaacact ttgttatcta
    29641 tgaccttgta aaattcttct tctgtaaagc cttctttgag tcttgcatca atcaaagata
    29701 tagttttctt tgttgtactc ttaaaagatt tatctgctgt tttatttaag taatcaacta
    29761 ttcttttata tatttctttg tcttttgtct tttcttcttt ttgctctaca gaactatata
    29821 tattattagt taaattagtc ttgttaatat tagtcttgtt actcggcacc tcattcctaa
    29881 catgtacagc accacatgcc gtagggttaa gcacctcatt cctaacgtta ggcattgtgt
    29941 gctgtacctc ttgtttattt aaaggactat atctatcaat atcattaata gaataatcat
    30001 taatagaata atctttgttc cggtattgat accgattagg ttccggtatt gataccgaat
    30061 ctatatcggt attgataccg aatcgttccg gtattgatac cgaatcggtg tttatactat
    30121 cgtcattgtt agttattttt tgttttaact tataaaactt aagagtaaat gcaaaataac
    30181 ttctaactcc tgcaactgga tttcttattg ttttattttt taaaagacca atatattttc
    30241 tatttttaac tttttttaac attttttcag aataacattt cattttttcc tctagctcta
    30301 ttggtttttc tcctaaaaca tgaagcatta tatctcctaa tctatctttt gatattttta
    30361 atattggcaa atcttcaagg aatttattat aattaaccca ataatacata tatccttctt
    30421 cataaaaaga atccattttc ccactgtgaa caaaatcctc gaaatgcctt aaaatcaaaa
    30481 gttcatcatt agttaatcca aattcaacag ctgttttttg gtcaaaacca tgtatactaa
    30541 acctcaaata aatcacccct ctctaacaat tatttattaa gaaagtaact taagttcata
    30601 gtcattaaat atcaaatctt ctttagttaa agttttatat attaattctt ctatttcttt
    30661 gtcgtagtac atcaatacta attctttttc atctgcatat gtaacaatgc aatttctatt
    30721 gcaactgaat tttgtttcgt ataattgaac aatttcacct ttttgatatt tattaaaatt
    30781 agagatattc attttacaac tcctttagtt ttgaatattt tcttcttcta tgtcttgtat
    30841 ttcttttaat atattcactc tattcagtgc taatttaagt ttagtagggt caaggagttc
    30901 tatctttctg tatatttctt ctctcatttc gtttatgttt accaatttat tcaccccctt
    30961 tttgttgatt ctgtaatata attataattt aaatatgctt acaagtcaac tactttgtaa
    31021 tacttttttt atttttattt gttgacttgt aagcatattt aaattataat aaccatatgg
    31081 aggtataaca atgatagata aaaggattcg agaattaaga ttaaaattga atttaagtca
    31141 agatgagttt gcaaatgagt taactttaaa aagaaatact atttctttaa ttgaaaatgg
    31201 caagagaaat acaagcgaaa gaactttaaa tgatatttgt aaaaaatgga atgtttcaga
    31261 agaatggctt aaaaatggta caggagaaat gtttactatt aaagatttgg acgaacaata
    31321 cgcttctttg gttggagaat taagcaaaaa agaaaatgaa gttgagagag aaattatttt
    31381 gaaaatgttt aaattaaata aaaagtattt aacggtattt gataaaatga ttgactcctt
    31441 actagagata gaagaatagc gtacgctatt cttctatctt tttaaaaaac ttattatgta
    31501 attttttatc attagcaaac ttcttttgtc attatgaatg tctaattttt ttataacctc
    31561 tattatctct tgctttatct cttccataat tcttcctcct attttaaata ctcttaatta
    31621 aattcttaaa accctttaaa aactgcatga ttttgtattc agctttgttc tacactctaa
    31681 tattaacacg gaaaaccatt ccaaaaatat tcgtacgacg aatattccga ctttaattta
    31741 caaaatttac caattaattt gtctattttc taaattgtaa aaaatttcta ttgattttaa
    31801 tattttttaa tactatagtt taatttcgac atggagtctt tattaaaata tcatagaaaa
    31861 aagaaaaaac tcactcaacg tcgattaagt gaattgacag gaataagtca aagttacatt
    31921 tcaaggatgg aaaacaaaaa atttaaacac agcccaacct taactcaaat aattttatta
    31981 gctgatatat tagaaattga ccaattgatt ttatctgaat tttttttgga aaaagaaagg
    32041 gagcatttaa aaaatactaa aaaatataat aaaaaagcag ttaaaacact tagcagggat
    32101 tttttaaaag aatataaaaa taaggactag gtaattattt atacctagcc ccttttttta
    32161 ctgctctact ttaatttctc ctagctcaat ccagttatct ttatataatt ccggaaattt
    32221 cttttctaca tctgattttg atgctccttg atttgcgtac tccattgctt caaaaagtct
    32281 tgaataatct ttcaggttat attttaaaac tatttctttt acattcatat tcttttcaac
    32341 taatatttta acttgtgcat caaatttgta gttagctggt acttgttgat tgtctaattt
    32401 atcagttact tttatgtctt taaattcgta accatctgta ccgactgcgt tccatcctgt
    32461 ttgaaataat ttaactgctt tatctgtttt attttctact tctacattta caacttcgta
    32521 cacataatca gctgcctttt tcttttcgat tccatcacta aaatattctc cattttcgtt
    32581 aaaacttccg tcttcgttta tatctccttt tatatcttct tttgtaacat cttttacagt
    32641 tattttaatt ccatcaacct cattttcatt tgctttcttt tcttttttag ttgtgtttcc
    32701 agtatctttg ttattagttg atgaacaagc aaccaaccct acacttaata aacatactgt
    32761 aattaataat aatacttttt tcatcctcga ttcctccatg tttttatgtt ttgttatata
    32821 tttcaacatc aaaacttata ttcctttaat tttctattct agtagtatga aaataattta
    32881 aaagaataat acctatttat ttccattcta agcatttttt tatgctattt aacataaatt
    32941 atttataaca aacagaaaaa tcgtttctgg tgtcttgtat cgcttctaaa tgatattcta
    33001 gtttcttaat caaataatca ttttcttttt atccaaagat acattctaat agtttcttta
    33061 cttttgaaaa atgctaataa tattttaaat agcgaccacc actttttgag acaagttaga
    33121 tttctaatta agttaagtac tttaataaat aaatacaaaa gatacatatt ttgttctcta
    33181 taatacaagt tctctataat aatatattct ctatatattt actgcaccac tttttgcact
    33241 accaaagcac cactttttgc actaccaaag caccactttt tgcactacca aagcaccact
    33301 ttttgcacta ccaaagcacc actttttgca cgtagtaaga aatctaatta atttttaaaa
    33361 acttattaac atgttatcaa cataaaaata atggttatca acataaaaat caaagttata
    33421 aacagcattg ttatatttag acataaaaat agaccttttt taaagaaggt ctatttttta
    33481 cgtgcatttt tccattctga aaaactacat agtctatata atttttgtat cctaggaact
    33541 tttttattct tttcttcgtc ccactcaaat ttattttctt tttttacttc gatatatttg
    33601 cttacttcaa atactgttat aatttttctt attttacttc tgttattttt gctatcacca
    33661 cataacccga ttttttcaca aataaatttt tcagtcatac atttaaaact attttcagtt
    33721 gtcatgtaac ataaaaggca ataaactttt attgcattag tattaaatgc agaaattaat
    33781 tcttgtagca tatcatgatg aattgtaaca aatttatgaa cgttatcgtt gtatcctgta
    33841 cttgtaccat agtttaatct ataaattatt ccgttgcggc tgttttctat ttcaagtact
    33901 ttacaatcta aactattgag cttttttata tttctctcta aagtagtacg agcaacccct
    33961 atttgtttag ctaattcttc cattttaaat ttattttgat acaaatatct atttttttct
    34021 atatgcataa ttctcatgct ccctttactt ataagcatta aagcgccaaa ctctttagca
    34081 caataatttt tttcatttaa acaatacgta ggcattggta atttataatt tttttctaat
    34141 tcttttgctt cttctaaaga cattttattt cccccttttg acggggcttg aagcactttt
    34201 aactaattaa gttaaaaatt tcaaattatt tttaacctta aaacattgat ttataagttg
    34261 ttttaatcta taatataact tataaattag ttaaaaaagg atataattat atcctataag
    34321 agcttcaagc tccgttggta aggaataagt cgagtcgcca aacaaacgct tattcctttt
    34381 atttgttttt gtaatctgta ataagttaat attaattata gcataaatat aaaataaaaa
    34441 aatatattga ttttgaaaat aaattatatt ttaaaagact tagtacactt actaagtctt
    34501 tttttatgta aatatatgtt ataataattt tagcgaggaa aaaagtgatt gaaaaagcgt
    34561 aagagtggct atttccatat ttaaacgcca aattccataa cggaaggagg tggaacaata
    34621 tggtgataaa ttttttattg agtatactag ctggtgttat atcagcttcc atatatgata
    34681 aaataaaaaa ccactcaaac gccaataaga gtggttcaag aaaataagtt atttttcaaa
    34741 acattggaaa tagctactct tgtgtaaagt aaatcattat ttccttgctt ttattatatc
    34801 acatttttta aaaaagatac aattttacat cacttcatat atgttaatta gtttaaaaat
    34861 acaaaaccac tctgcgcaaa agagtggttg ggaatttgat ttaaaaatca aattcaaaaa
    34921 gtctagataa ttttttactt tagacgaaaa ctctactctt atgactgaaa tacagttctc
    34981 ttgcttttat tatatcacat tttctttaaa agatacgaat ttatatcatt tctaatatac
    35041 taaaatcttt actttttacc aaaatttgtt taaaaatagg agaaaactat tgcttttgac
    35101 gccccgtcat gatataatat atttatagag aaggaggtga gaaagcaaaa aaaaataaaa
    35161 gaaaggagga acgggaatgg ggagattaga aagaagtaaa aagaaaagag aaaacaaatt
    35221 taacatagtt aaaaaagttt tctctttcat cctattatta cttaacataa tcctcgcagt
    35281 tctaagaatt ttaaaagaac tgtaaggata agcctagagg gaatagccct tctctctagg
    35341 tgacttcttt ctaatattat aacacatttc taaaaaacat gagtgataaa ttttataaga
    35401 tttgcattgt tttagttttg ataactatag tcttaaatat agtatctata gtattaaact
    35461 ctagtacaag caacatgctc ggtttagttt ttagtgttgt attattgctt ttctttgtaa
    35521 tacagcaaaa aaggagctaa tgaatgaaaa aagaaaaaag agatttaaag tttaactttc
    35581 ataaaggtgg gagtgggtcg tttactccta gaatgagtgt tcctaaaaaa tgggctgatg
    35641 aaatgggcat aacaagagaa aaccctaatg ttacggcaac attcgagggc gacaggataa
    35701 taattgaaaa ggctaagaat gaatgacttt ttaaagaagg ttgatttttc ttagccttct
    35761 ttattgatta ttaaaaaaat aatataaaat tatatataaa
//
LOCUS       DAMKMI010000023        35192 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMKMI010000023 DAMKMI010000000
VERSION     DAMKMI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.024"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            245..1219
                     /locus_tag="PC429_001102"
     CDS             245..1219
                     /locus_tag="PC429_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846617.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(1335..2396)
                     /locus_tag="PC429_001103"
     CDS             complement(1335..2396)
                     /locus_tag="PC429_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF3846618.1"
                     /translation="MKKIRKLTTKLLITTIVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(3008..3859)
                     /locus_tag="PC429_001104"
     CDS             complement(3008..3859)
                     /locus_tag="PC429_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3846619.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(3876..4796)
                     /locus_tag="PC429_001105"
     CDS             complement(3876..4796)
                     /locus_tag="PC429_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF3846620.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(4906..5820)
                     /gene="murQ"
                     /locus_tag="PC429_001106"
     CDS             complement(4906..5820)
                     /gene="murQ"
                     /locus_tag="PC429_001106"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF3846621.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(5919..7016)
                     /locus_tag="PC429_001107"
     CDS             complement(5919..7016)
                     /locus_tag="PC429_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF3846622.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDIGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLTGCHNFYPHRYSGLNLEH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(7249..8595)
                     /locus_tag="PC429_001108"
     CDS             complement(7249..8595)
                     /locus_tag="PC429_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3846623.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPELLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(8684..8992)
                     /locus_tag="PC429_001109"
     CDS             complement(8684..8992)
                     /locus_tag="PC429_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846624.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(9254..9652)
                     /locus_tag="PC429_001110"
     CDS             complement(9254..9652)
                     /locus_tag="PC429_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846625.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(9654..10814)
                     /locus_tag="PC429_001111"
     CDS             complement(9654..10814)
                     /locus_tag="PC429_001111"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF3846626.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(11245..12264)
                     /locus_tag="PC429_001112"
     CDS             complement(11245..12264)
                     /locus_tag="PC429_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3846627.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRNNTMFEDVFINKFNLEKLIKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LSLDS"
     gene            complement(12258..12938)
                     /locus_tag="PC429_001113"
     CDS             complement(12258..12938)
                     /locus_tag="PC429_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846628.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVGLIDDFEVVLDEFIDR
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(13090..14997)
                     /locus_tag="PC429_001114"
     CDS             complement(13090..14997)
                     /locus_tag="PC429_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3846629.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQDENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNVYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(14998..15750)
                     /locus_tag="PC429_001115"
     CDS             complement(14998..15750)
                     /locus_tag="PC429_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846630.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFISIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPTVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(16066..17220)
                     /locus_tag="PC429_001116"
     CDS             complement(16066..17220)
                     /locus_tag="PC429_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3846631.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(17223..17690)
                     /locus_tag="PC429_001117"
     CDS             complement(17223..17690)
                     /locus_tag="PC429_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3846632.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(17704..18192)
                     /locus_tag="PC429_001118"
     CDS             complement(17704..18192)
                     /locus_tag="PC429_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846633.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSSF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(18206..19537)
                     /locus_tag="PC429_001119"
     CDS             complement(18206..19537)
                     /locus_tag="PC429_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF3846634.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     DRQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(19551..21116)
                     /locus_tag="PC429_001120"
     CDS             complement(19551..21116)
                     /locus_tag="PC429_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3846635.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(21286..22071)
                     /locus_tag="PC429_001121"
     CDS             complement(21286..22071)
                     /locus_tag="PC429_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3846636.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(22238..23437)
                     /locus_tag="PC429_001122"
     CDS             complement(22238..23437)
                     /locus_tag="PC429_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF3846637.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEEMSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(23959..25296)
                     /gene="xylA"
                     /locus_tag="PC429_001123"
     CDS             complement(23959..25296)
                     /gene="xylA"
                     /locus_tag="PC429_001123"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF3846638.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(25392..26942)
                     /gene="xylB"
                     /locus_tag="PC429_001124"
     CDS             complement(25392..26942)
                     /gene="xylB"
                     /locus_tag="PC429_001124"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF3846639.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            27150..28310
                     /locus_tag="PC429_001125"
     CDS             27150..28310
                     /locus_tag="PC429_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF3846640.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(28466..28870)
                     /locus_tag="PC429_001126"
     CDS             complement(28466..28870)
                     /locus_tag="PC429_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846641.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(28891..29373)
                     /locus_tag="PC429_001127"
     CDS             complement(28891..29373)
                     /locus_tag="PC429_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846642.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(29393..30202)
                     /locus_tag="PC429_001128"
     CDS             complement(29393..30202)
                     /locus_tag="PC429_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3846643.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(30195..30974)
                     /locus_tag="PC429_001129"
     CDS             complement(30195..30974)
                     /locus_tag="PC429_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3846644.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(30974..33052)
                     /locus_tag="PC429_001130"
     CDS             complement(30974..33052)
                     /locus_tag="PC429_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF3846645.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(33851..34372)
                     /locus_tag="PC429_001131"
     CDS             complement(33851..34372)
                     /locus_tag="PC429_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF3846646.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            34538..35104
                     /locus_tag="PC429_001132"
     CDS             34538..35104
                     /locus_tag="PC429_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3846647.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTIGTVLGAVV
                     LGPFWSALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
BASE COUNT        11569 a         6124 c         3784 g        13715 t
ORIGIN      
        1 ttgattattt tcatttgcta tttaaaatca attttaagtt ttcttcttat aataaacttc
       61 ctctttgtgg tctcatgtta gtctctaaat ttaaaatttt acaaaaaatt ttcataaaat
      121 aaaattatat tgattgtatc taaacaaata gggcaataaa ataattaagt atagttactt
      181 acctttcaag caatattagt tttatgttag aattttatta tattgattta caggaggttt
      241 gcatttgaat aatattaatt cacttaaagt aataaaatta tctcttggat gttctcttgc
      301 tatatttatt gcttggctat taaaattaga atattcaatg gttgctggag ttatagtact
      361 acttacagta aaggacacta aaagagaaac tcttaaaggc tccattggaa agatttatgg
      421 ttttctttta tgtactattt tttcatattt atgttttaat attttaggat ataatttgac
      481 ttcattttca atatttatat ttataattat accactttgt tttttgttaa atattcaaga
      541 tgttattgct atgtgtgtag tcatagcatc ccattactac cttcaaggag aaacttctat
      601 caaatggatt ttaaatgaag ctggaatatt tgcagtagga actggaatag gtgtcttaat
      661 aaatatgtat attccaacaa atatacataa aatccatgaa gttcaaaaaa agttacagga
      721 agaagtgagt attgtactta tagacattgc agatattatt gtaaatccta aaaaagaaaa
      781 tggatatagt agagatttat atacattaaa ttcacttatt gacagcagta tttctgaaac
      841 ttacgataat attaataata ctttattaag tgatactaga ttcttcttag aacatatgga
      901 tattataaaa agccaacggg atatcttaga aaatttgtat agttatgtat ctcaactaaa
      961 ttctacacca ccacaagctc atattctatc tgcttttata cataagatag gttatactga
     1021 atttgaagct gaaacaggca atcttcttct tgaagaatta aagagactaa tgcttagtat
     1081 gaaaaaccag cctctgcctg ttgacagaac agagtttgaa aatagagcta ttttattcct
     1141 atgcttgact gagttaaaac agtttttagt taatcgtaaa catgctcaga tgcttagaga
     1201 taataatttt tataaataaa aaggggagaa atctagcagt tgacctgact acaaaatatc
     1261 agatataata ttaaatgcgc ccctagtaag agggcgcaat gtttataact tattctactg
     1321 tcatgattaa atcattattc atatcgaaca tacattattt gaccaagtcc aatattagct
     1381 ttttcatcag tgatttctat tttaatagtc acatcatttt ttttacagta cttcgctaat
     1441 tctgcaccag aacgattttt accactaaaa tctaaagaaa atggcttatc atatttaaaa
     1501 ggatattcaa caggtttacc ctctggtgct aatgcaaaca tcccatatgg tgtgttcata
     1561 aatcctatta cagcaatcgt aagatttgat gggtctaaat ttgattcact acctgtaaat
     1621 gtaattgtat ttactgatga attaactaca ctacatctaa caccttcata cattacatgt
     1681 ttttcattta tatcttctcc tttttttcca aaatttccaa cagcctttgc cccttcttga
     1741 tgtggtaaca tagtgtgctt tccaggtggt gctggtttct gtgtctgttc tggttttacc
     1801 gtctgtacta ttgcaccaac ttttggatat gctgtcttat caaatggtaa tgggtggcta
     1861 agactcttgt atatgatatc ctttcctgct gatttattag catcaatatc tacatgatac
     1921 aaacgcccat caccaaaacg gataagattc cactggtggt catttgatgt gctgtgtaca
     1981 taataagatt caattcccat agcatcacac aaagctttca tgctcctagc cattcctgag
     2041 catgcacctt ttttagtaac caatgtatag gcagtatttg ctccctctac tgcattccag
     2101 tcaacatatg ttgtgttatt tttaatgtga ttgtaggcaa ctaaaatctt agcatctgtg
     2161 gtcatattct ttgtgatttt cttgttcata aaatctgctg ttgcttgtgc tactgagttt
     2221 agttgggctt gtgacagctt tggaccatat acgctattgg ttccagtgac atagcttttt
     2281 ggtgtttttg ctgttacctc tttggctgtc ataccataaa ctgtcatact catacctgag
     2341 attaaaacta ttgttgtaat caataacttg gtagttaact tcctaatttt cttcatactt
     2401 aatattctcc tttataaaac attgttactt cctattctct accattaata aaattaattt
     2461 agtatttaaa aaggtggatt gtcaacatgt atatttctcc attcatacat tcgtgaaata
     2521 tttaaatttt atggaaaatc taatagatat tttctagata atttgtatac aatacaactc
     2581 gcaaatcagg gtatttgctg ttagtttgag aaaatgatga tgattaatac attcggaatc
     2641 tttgacctat aataaatacc attgtcttca atcattaata atggtaagag taaagagaat
     2701 tatcaataat acagttaaaa tcaactttgt atacatttca tacattctct cccaacaaca
     2761 ttatacttct tataatttgt atattcaata cgttttctgg gaaaatatag gatatatcta
     2821 taaaaatata aaaaaggagt tgaacttaaa atttgattaa aaaaaatcaa ttttaagaca
     2881 actcatttgt attataaaaa tgctatatat tattattaag taagtcaata ttttagatat
     2941 ttatctattt cttattgact tacgcttata gcctaactta tttgtttagt tgatatataa
     3001 cctagtttta agcattatta atatttaaat aatcaagtat cctttttgtt tcttgtagtt
     3061 tctgttctgc ctcatttaag ttttcttgaa ttatagaaat atatattata tctatcaacg
     3121 taagcattgc tattcttgaa gaaatagctc cttctctaag tcttttttct atatttggta
     3181 ctactagctt taaatcacta atcttactaa gatgattgtc actatattta gttatacaaa
     3241 tgaccttaca tccttttaat tttgcatttt ctatagactt tattacttct tttgattctc
     3301 cagagtaaga tattgctata gctatatctt ctttatctac atttgaagat accataagtt
     3361 gtgtatgact atctaaagag gtaaatgcct gtttatttat ccttaaaagt ttcatctgta
     3421 aatctagtgc aactaaagcc gaaccaccta ctccaaatat atatacattt ttagccttta
     3481 ttatctgttc aactgcttct tttattatat ctttttcatt caacaacctt gtttggttga
     3541 tagtctctat gtttttgttt gtaattttgt ttataacact atctatatta caatcatagt
     3601 tttcatctac atatgtgtat tcattttctt tgcccatcaa atctttagct atctcaattt
     3661 ttaaatcccc aaaacccgaa tatcccattt ttctagaaaa cctaactata cttgctgtac
     3721 ttgtttttga gtttgacgcc aattctgata cagaaagctt acttatagcc tctgtattat
     3781 taagtatgta atcagctatt tttttatcta cttccgttaa atcttcatat atctgtttta
     3841 attttaaaat gcaactcata ctctatcacc tctacctatt tactataata tttcttctta
     3901 gcaagtaaac atgctcctaa atctggagtc aacactggtc tcactatacc aaatctcgtt
     3961 ttattcaaat attttttaag tggctctaat attaaatctc ctgacttaaa cactccacct
     4021 gaataagata cctttatctt tgtaccttgt tcaaaatgat gctctaatcc tattataagc
     4081 ctacttaatt cataagctgc atcatcaaat attttactag cagtattatc accttcaata
     4141 actgctttta aacatatcgt agcaaacttt gctatttcta ttctatctcc ttgtatttca
     4201 tcatttacat attttattat gtcataatca ttctctatat taagatgtct ttttattaaa
     4261 tcatataatt ttgtttttga acaccttcca tcagactgca ttgtgaacat ttttaaggta
     4321 gccattccta tataatacgc tgaaccttca tctcctaata catagcccca tcctccacat
     4381 ctgtgaagat tcccatcttt atctaaagcc tgtgctattg aaccagtacc tgcaattata
     4441 tttatacctt tttccccatt taaagaacct gctaatgcta tttctacatc attacctaga
     4501 gtataattta tatgactata tgcatttttt gttgctactt ctaatgcata caatacttct
     4561 tttatttttc catatccagc aagtccaaca aatgcatacg ttatttcttc tacatttatc
     4621 ttagcatctt tacaaacttc ttccaaccca gttattaata atttcgttag attatcaaat
     4681 cctatttgat tataatgaca agttcctttg gttatggttc ctactatatt taattcttca
     4741 tctactaatg taaatgttgt tttcgttcct ccaccatcta cacctaaaaa atacatacta
     4801 tcctcctaca atatctttaa taattctttc aatacttaat atctaaaaat aaccttccaa
     4861 tgctgttttt ttaatattga aaggttatca gtactattat ttattttaca atttttctat
     4921 tgattttaaa gcttcactta tataaccttc ttgtctatct aggatactct ttgcactatc
     4981 cttatctaaa ccacttttta tcataaatat agccaattta acgctattat ctgcttcttc
     5041 taagaatttt tctgcaacat catattttac atttgtagct atcattatta ttctttttgc
     5101 tctttcaacc aacttttcat ttgatgtctt aacatctacc atcaagtttg agtatacttt
     5161 tcccatctta accatagaag ctgttgttaa catattacat accatttttt gagctgtacc
     5221 acttttcatt ctagttgaac ccattattac ttctgcccct actataatgt ttattggtaa
     5281 atccgctttt ttactcattt cactattcgg attcatagtt attgatagta ctgcacaccc
     5341 caattcctta gcatactcca ttgcacctat aacataagga gtccttcctg atgcagctat
     5401 accacatata acatccttag atgtaactcc tctatctatt atatcttgct ttccaagttc
     5461 tttatcatct tcagcacctt ctattgcttt aaatattgct tgatttccac cagctattat
     5521 agcttgaaca agctcaaaat ccactccgta agttggagga cattctgtgg catctagtat
     5581 tcctatccta ccactggtgc ctgctcctat atagaataat ctacctccct gcttcattct
     5641 atctactatt atattaacag cttttgctat actctccttt tgttcttcaa cagcataggc
     5701 tacagtttta tcttcgttat ttattaattc tattatgcct aatgtatctt ctttatctat
     5761 ttgcaatgta ttcttatttc taccttctgt aactaaattt tctaaagttt ttattaacat
     5821 atcatttcct cattttctta atattaaatt tttaaacaca ttttaattcc aactttcata
     5881 attatatttt tataataaat taatattttc ataataaatt attttgttga gggtttaaaa
     5941 ttaaactttt gccatggttt aatataatct aatataaata attcttcttt tcttatatat
     6001 cctactacat tcgatttacc actgtttttc atatcagtca aggctatttg taactcccct
     6061 gcataatgac catattcact actttcaatt actatatctc ctctttttat tattttagga
     6121 gtattaaata gttcaaattt gtatccttta tatttaacac gagattgagt agaccttatc
     6181 agactgtcag atatgtcccc tctattaaaa tgtatttctt caaagagtat cttcttctca
     6241 acttctcgta tattttcctc tattgctaca tcaaatgtaa ccatatcttt tctcatagaa
     6301 cctaatacct ttagctcctc atatgttgga taacaatttg atattattat atcatttata
     6361 ttattcatag ctattatatg ctttacttga atatctatag gtagatttct gtgcatctca
     6421 attgttggaa gcccatcagt tacaggccat ggtccaaatg tagtttcatt ttgacttgtt
     6481 acaaaaactg ctgttcttaa tccatactta ttaaatcttt ctgtacattt tataaagtgt
     6541 tctaaattta atccactata tctatgtgga taaaagttat gacatcctgt caagttttct
     6601 ttatttggtt ggtagtccat tattgtatct atataattag tattattact catattgatt
     6661 tctattttaa gattttgagg attaaaagtc attaatgcct cttcagaacc tgtaaatcct
     6721 atatcaagcc ttattccgtc tgccttcgtc tttttaaaaa aagtcaaatc tttgtagctt
     6781 atttctaatt caccaaatac tctgggattt acatcaagta ttacctcaaa acctttaaac
     6841 ttagcgtatt catttatacg tttaaatttt ttaattatat tttctttagt atctttaact
     6901 gataataaac atgaaaatat acgtgtaaat ccattttcag cagctttatc tatataatca
     6961 tatatttcct tagatgttgt tttttctgga taaattgata ttcctaatct tcccatctgt
     7021 gtcatctcct tatatcctat atgtgtttaa atttatttcg aattacacga aactttttta
     7081 aaaatttggt gaaaaaaagt ttcatatata tttataaaaa aagaaaagtt taccttttct
     7141 ttttttataa acaatcaaca aaatatcttc gttacttatt tcataactgt tatactttga
     7201 aatttatact aatctaatat agtttttaat ttccaatata gtttctaatt aataagcttc
     7261 ttgctcttca actttttctt catgaagcaa tctcttatcg tacatcttga caaatggata
     7321 gtaaattatt attgatatag ctatacataa taagttaaga actgaagctc tccaatctcc
     7381 accagtggct aaatatgccc ctactggtcc tggtaatgtc caaggagcag taactactac
     7441 tctggatact aaattagcac tcatagcaaa ccatgaaact gttgccatta ctattggtgc
     7501 tacaacaaat ggtattatta atattgggtt taatactatt ggtgctccaa atattattgg
     7561 ttcatttata ttgaatattg ctggtattac agttgctttt cctaaagact ttccataagc
     7621 tgattttgct ctaaatgcaa gaagtaaagc taagcctatt gtagctccag aacctccaat
     7681 ccatataaac cattgaaaaa agggttcagc agcaatacct ggaactgctt gacccgcagc
     7741 atatgaagct gtattagcat ctaataattg tatccataaa ggtcttgcca atgaacctat
     7801 tattgataca ccatgtattc caaatgacca aaaaaacgtt attaaaaata ttagtataat
     7861 tactgatggt aatgtgtcag tggctgaaac taatggagtt actaatttag ctatgaagcc
     7921 atgccaatca aaccctagga aataagtaac aatagccatt aaaattatta ttaatgcggc
     7981 tggtgtcaga gcttcaaaag accttgtgac tgatgctgga actgcttctg gcatggttat
     8041 cttgaattta gatttgtcag taaatctata aatctcaaca cctattatag atgttacaat
     8101 agcaacaaac attgaacctc caccaagatt agccattggc atttgccatc cttgaggaat
     8161 tgaattatac caagctaaaa gttctggact cttttcaact atctttaata actcaggggt
     8221 aagaccctct attgcagtag gaactattgt caaaaggaat gtagctgtag ctaataaacc
     8281 tcctgtcaat ccatctaact tatatgactt tgccaaacta taacctatgc caaatgtaga
     8341 atatactgat ataataaaaa atgtcattct atatggtagt aatattgtaa ctgcattttc
     8401 ctttagatac tgagttaccc cccatgaatc tggtaatgga ggaaatgcta atatcaagaa
     8461 aaatgaccca actattataa gtggtaatga agctacgatt ccatctctta cagctcttaa
     8521 gtgacgttgt tctgccaatc tggccataga agttgataat ttagtgtcta aaaattctgt
     8581 aaatttatct agcatctttt ctctccttaa ttaatattta tttttattat cattaaaatt
     8641 tttataaaat caactttctt ctaatctaac aacactgcaa ctactattta ctcaacagtt
     8701 cttgaacttg ttttaataac tttggtcctc ctaatggact ataagcttgt gcttgtatta
     8761 atgcacatgg tacactaacc tcatcagcaa aagcttttaa atagtcaaat ctatgtctga
     8821 cttgtggagc taccatagca atatcccatc catttttaac ttcattctca aactcttgag
     8881 taccaacagc caaaacatct atatctattc cattttttgc tccttctttt tgtaatgcat
     8941 taactgctat agcacttgac atccctgatg aacaaacaaa taatactctc atattgattc
     9001 cccctgtttt tttatttctt ttcaatcatt atagcatgta tttttgatta caggaatacg
     9061 tttgtatata ttttttcata ttttacattt taaattgtaa ttttatttca tgcatttata
     9121 tcttgcttta attgctttat ttatctaatc attcttttgt ctaaaattac acactactta
     9181 aaatactcat atcttttctt aaaaaagaaa aaagactatc ttattattaa cataaaatag
     9241 tctcaatact tttttaaata tgtattcctt tatatatcca gaagtaacct attattgcaa
     9301 atgataaagc aaataaaata tacagtctaa aaaatcccaa agaaaaacct ctcttagttt
     9361 caacaaagta tcttgtatat gcaactgcaa aagatgtaat taatgaattt cctataacaa
     9421 ctccaaatct attatctatt ccaaatatag ataataacaa aggaaaaact acagtactta
     9481 caagagatga aactattagt attaatccac ttttaaaact tatagttgga atattataat
     9541 ctgttctttg atttttctcc atttgtttct tagtattttt attacttttt gtactttttg
     9601 catttttata attattattt ttctttcttt tctttttttt attattagac atgtcattta
     9661 cctccaaaag gtttaggtgt tatattccct aaaataactt tatttttagc ccctgttgca
     9721 gatattacat tagaaaaaga attatttaat gtttcattgc ccaagattac aaatgccaaa
     9781 gcctcctttg catcagaaga atacccatat tcatcttgag ttaatacttc aacctctaat
     9841 aataactctt ttatatatcc tattaaagtt agattatgag ctcctccacc tccaattaat
     9901 aaagtatcta cattaacctt tggaagtatg aagtttttat aattataata tatagctttt
     9961 gccgtaaaca tagttactgt tgctatcaaa tcatatttat ctacatgttt atatttgtta
    10021 agcatgttat ccaaataaac ttgtccaaat acctctcttc cagtgctttt aggtggcgct
    10081 tgactaagat acttatgact catcaaatct tttaacattt cttcattaat tttacctttt
    10141 gatgcaaagt atccattttt atcatacttt ctattaaata atctttgaca tactccatct
    10201 attatcatat ttcctggtcc tgtatcaaat gcaaatacat catctatgtt acatgattta
    10261 ggtattatag taatatttcc tattccacca atattttgaa gtgctaaatt tttattgtta
    10321 tcactatata ataaaaattc actatatggt actaatggag caccttctcc acctgctgct
    10381 atatccatta ctctaaaatt agatattacc tttgtatttg tttcatatgc tataactgct
    10441 ggttcaccta tttgaagcgt tgaagataca aaattattat aacttcttgg tatatgaaag
    10501 attgtttgac catgagatgc gataaaatcc aaatttgcta tatgaaagtt tgctttatga
    10561 cacactgatt ttacagcttt actaaataaa tatccaagct taaaatttaa gctacagatt
    10621 aaatcaacac tcgattcctc ttctatacag cactttctta tttcgtcttt tatatcttta
    10681 ggaatttcta gagttataaa ttctatttgc ttaactttag tatttctacc attcccacta
    10741 atttcagcta gtactgcatc aactccatct aaggaagttc ctgacattaa acctacacta
    10801 tacatagtta tcatcctttt aagtttatag ttattcctcc ccaaatcaag gtattaagtt
    10861 taataatttt agaattaatc ttatttcatc tttaattacg ataatttact cttatgaaat
    10921 taattgagtt aatcttatac acgtttttta ccttagctaa atcttacatt aatttatttt
    10981 cagtgtcaat gtaataccat gtaatttttt ttcacaaaca aaacatattc tctctccaaa
    11041 aaccatatta aaatagttac tacaaataaa aaacatacca ggtacatatc tgatatgttt
    11101 ttcatatata ataattatta ttttataata tagatagagc atcatttaag gcactaatta
    11161 aatcttcact attttcaaga actaaattta ttcttattgt atatgtcgat ttgtatatga
    11221 tgatagattt tatagtctaa taacctaact atctaggctt aaataatctg atatatcata
    11281 aaaatagata ttaaaaattg tatatgaacc attttccgaa catacctcaa tctcatgccc
    11341 taaactatct atcattttct ttacaaaata catccccatt ccagtagact tatacaattg
    11401 atttcttcca tttttccccg taaatccctt gtcaaaaacc ctagatatat cttcctcatt
    11461 tatacctata ccaaaatctt ttatagataa taccactcca ttttctaact tttttgaatt
    11521 aaattcaatc ttaccaactt cctttgaata ctttatagca ttattaataa tttggtctaa
    11581 aacatatgac atgcatttag aatctgtgta aacatcattt tctaattcat tcaaactaac
    11641 ttctacatta ttttttataa gcaaaaatga attatttttt atagacattt ttatcaattt
    11701 ttctaaatta aatttattta taaatacatc ttcaaacata gtgttgtttc tactactata
    11761 caaaatagag tttactaaaa aatttatctt tgcaacctga ttcttaatat ccaaactatc
    11821 ttctatgcta cttaacctat ctgtaattat acttaaagaa gatattggaa tttttatttc
    11881 atgaacccat ttagctatgt actcatccaa ttcttttaat gattgtttat aagtctctat
    11941 gtcactatta ttttttattt ttaagttttc tataatatct tttattagat tttcttcaaa
    12001 agattgtcca tcaactaaat aactgtctat ttcaccacta ttttctatac aattatataa
    12061 atttgtgtaa ctatttctaa aatttatata atctataaca aaaaatacca ttattataaa
    12121 cagcactaaa aaatctaaat atattaactc tgaatattta tttttaaaaa tatctatagc
    12181 aaataagtag atatttatta tactaactac aactaaaggt agagcaatat acttagacct
    12241 accttttaaa tattttacta gcattgtatt atatatccta tccccttctt agtttttata
    12301 aaatcatcaa aacctagttc tttaacttta cttctcaatc tattcatatt tacagtaagt
    12361 gcattgtctg tcacaaactc atcattatcc catagagtca tcataagact ttcccttgat
    12421 acaaccctat tcttattttt taataataat gttaaaattt ttatttcgtt tttagtcaat
    12481 tctatacttt tatccttata cttatgtttt ataatccctt tccccacatc aaacatcaaa
    12541 tcttcacaat acaaaatact attagaatca ctataattat agactcttct tattatacca
    12601 tttattttgg taaccaatat ttctatagaa aatggtttgg taatgtaatc atctgctcca
    12661 ttatttatac ccattattaa atccatatca gaatctcttg atgataaaaa tattaatggt
    12721 acatttgaaa tagctcttat tttttcgcac cagtaaaatc cattatataa tggtaaattt
    12781 acatctaaaa gtactacatc tggttttcta tctataaatt catctaatac tacctcaaaa
    12841 tcatcaatta atcctacttt aaatccccat ttttcaagtg ctaatgcaat ctcttttgat
    12901 aaatcaatat cgtcctctac taataaaatt ctatacattt gtatattctc cattatccta
    12961 atatttcact atgctatgaa ttattaatat cataacatat tttatatgaa tttattaccc
    13021 aaattattta gttaatcaac aaggagactc ttattacaag aatctccttt ctctttatta
    13081 atatatcaac taatttttat acactacact cttaaactta ttaacagata ctgctccata
    13141 tattatgaat attatcaaac atatcactaa acttagtata tatataccaa ataaatcggt
    13201 catcaataaa tctcctgcta atctcaatgc aaaataacta gatatagttg cacttaacat
    13261 tggcaaagta taaaaaatag ctacttcttt aagtatagct ctatttatat ctttttctgt
    13321 agtacctatt tttaacaaaa tcttatattt ctctttatct tcatatgcat cactatatat
    13381 tttcatatat attatacttc cagctacaat tgcaaagact aagaataaaa acacaagcac
    13441 tacatatgca aacttcatcc attgactctc accttgagct ttatatccat tttgaatata
    13501 cacatttttt aaacttaaat tcttttcaat attgttgaac attacttgac tattttcttc
    13561 ttgaaatacc tttgcaccat aaaaatacac ttgttgaccc ttatctctta gtttaaaata
    13621 tgtatcatca tttaccacta taatactttg attaagtcct gtccctagta ctggaacttt
    13681 tacttctccc cttgaaatat tatataactg accatcaagt tttatcttgg cttcttttgt
    13741 agctattgat gctatcgttt gagggctttt taactctatt acattatttc cactaactaa
    13801 tctttcacca tactctttta attctttatc attgccattc actttaagag tattacaaaa
    13861 atcactatat ttcattatag tatctgttgt atcataagct tcttttatat ctgttttctt
    13921 taaaattgtt agttctggat ttactgagta ctttatctta ttagtatctt ttattatatt
    13981 ctctatttgt tttttattca cttcatcttt atccattgaa actatgctaa tagtgtacac
    14041 atttctttgt tcttgggcat tcttatgtat acgcttcatc gcaactgaag caccaagagt
    14101 tgatattgtt gtagttataa ttatagctat tatagcataa gttttatagt ttttatttaa
    14161 tcggtagttt atattgttta ttgcaattat attatttcca ttaaataata tttttttatt
    14221 tttgattata atatcaaata taacaggcat aactgatttg aataatccat aagtacctac
    14281 tattattata cctataacag gtagtacact agttatatct ccagttctta tattggtaga
    14341 aagatagtag ccatatccta ttactataat tgatactata gctaatatat atatacctat
    14401 tcctaccttt ggtatttttt cttgtttttt agatatattg attagattta ttacactact
    14461 tctacatatt gttataaaac cttttacagc cattattata aatatagaaa taaagattaa
    14521 agtagcatat agtatagctt ctatactaag tccattagat acctttatat taaatccaga
    14581 taactttatt atcactactt gaaaaaatct agatgttatt atccctataa ttattcctgt
    14641 tatacaagaa cttattccta taagcatatt ttctacaaaa tatatttttc ctatagtata
    14701 taaatctaat cccataaaag acaatattcc aatttcttta gacctattct taaaaaatat
    14761 atttgttgca taccatataa agaaaaataa aaatactgat agtatcatta ttacagctac
    14821 taacactgat agtgtaaatt ttttattttc atcttgtaac acatttatag catcaccata
    14881 tcttaataat tcaaaattag ttagcacaac tactgaaaaa accatagata taaaaaatgc
    14941 tagataagtt cttatgttac tcttaaaatt attataagca attccaagta tactcactta
    15001 catctcacct cccatagagg ctagcatatc cataattttt ccataaaatt cttttctact
    15061 atttcctttg tttaatttac actttatatt tccatcattt atcatatata cttcatttga
    15121 ataacttgct gttagagggt catgtgtaat cattataata gtagcgttat tttctctgtt
    15181 tattttttct aatgattcta aaagttcata tgctgattta gagtctaaag caccagttgg
    15241 ctcatcagca aatatgactg ttggatttgt aattaatgac ctagctgctg cgcctctttg
    15301 tttttgtcct cctgataact gatatggata tttatctaaa tgattttcta atccaaattt
    15361 tttagctata gaaaatacct tagcttctat ctccttactt ttcttttttc ctaatgctag
    15421 aggaagtgct atattgtcca ttagtttcat attgtctaat agattatagt cttgaaatat
    15481 aaaccctatt ttttctcttc ttaattttga tagttcatta ttttttactt ttgatatatt
    15541 aattccatct agtattattt ctcctgaagt ttgtttgtca agtgtagaca ttaaatttag
    15601 caatgttgtc tttcctgcac cagatggacc cattattgat ataaaatcac cttcattgac
    15661 tgtaagagat atatttttta atacatttgt tttaaatcct tttattccat aatcttttga
    15721 tatatttttt atttttaata tttctttcat aataatagcc tccagttgct aattttttaa
    15781 tatatattta ttttaaaggt tttatataaa taaatcctta agtaaatatt acattttaac
    15841 tgttagcatt acacttttgt aacatatgga agactattat aattataatg gaaatatctt
    15901 aaaataaaag catgatttac ttctttgtat aaacaaagaa aagaagctcc taagaagcct
    15961 ctaatctata caattattct tctctataaa aaaccaaaat acacattgat tgtggcaaat
    16021 acagatacta taaacaagtg atattactac tatatattaa aacaattatt cctgaagata
    16081 attcaataag actatcaagc aataagctaa gcatccaaaa agacttaata ccattaatgt
    16141 ataattatac ccaatattaa tgctcaaaaa accagcaaca gttggaataa tcagagatga
    16201 acctcccata aatatactta aagctattaa ttggctacta cttctaagtt ttttaggaga
    16261 aacttcaaat attaatcttt ttgaagtaat taaaattaaa ggagtagaaa acacttgaaa
    16321 aacacacagt ataataatga tatttgaatc atttgttaaa gcaaataata gaaactgaac
    16381 tgttatcata atagcactta attttaataa gtttaaactg ctatattttt ttaataaata
    16441 agaaccagcc agatatgttg gtatctctaa caatgactgt aaagaccact ttaaagatat
    16501 ctgagaattt gtagcattta aagctatcat tttatctatt acagtacagt tatttgctac
    16561 caccattgag tataataaga ataaaataca tactagtaag atatattttt tatcttttaa
    16621 taattcaata atatctttat tagttacatt tattttctta ttaatctttt caatatcagg
    16681 taataatagt atatttataa taagtaatac tacaattaaa ccaatcgcta cagataatcc
    16741 aaaatatgag aatctaagaa tcaagtaaga tgctaccata cttccaatag cccatccaat
    16801 cgacccaaaa gtcttaataa atgacaaata attattaata ttatctccac aacctattat
    16861 ccatgtatca tataaactac agcaagtatt taataatcct cctgataaag caacaagtaa
    16921 taagtatatt ataaaagttg cagattcagt agaaaacata ataactgatg caattccata
    16981 aactgataaa ctaattaaaa caattttttt cattgtttga tacttatcag ctaatactcc
    17041 aaataataat tgaaacaata tattggttat tgcatagctc gataatataa ttcctctttc
    17101 aaacgctgag taacctaatt ttgataagaa aggtgtgaat tttgttgaag caaaggcaaa
    17161 aataatatat gagaaaaaat agaatattat aaatttaaat ttaatcgctt tattaatcat
    17221 aatcaatcaa aattgataac tttaatacca ctatttcctt cctgatttaa gtttgaaaga
    17281 tgatatttta ccgtattagg attctctaca ataactaaag gaatatataa agtattttca
    17341 agtaacttat tatcaatttc tactaacttt tcccccatat taacttcttg accttgttta
    17401 acttctaatt taaagttttc tttttttgtt gttgcactat ccaatccaat atgaataaga
    17461 atttgaacat cattttgcaa tttaattcct atagcatggt tagtttgagc aataaaactt
    17521 attactcctt tagtcggtga tttaagaata ttgtcctttg ctatcatagc aaatccatct
    17581 cccattacct tagttgaaaa tactttatca ggaatttctc caagattaat tatttttcca
    17641 tcaatgtatg aattaattgt aattgcttct ttttttcttt taaagaacat tatagacact
    17701 cctctacata attaacttta ttctaattat cattacaaat aacgatatta agcatactgc
    17761 aattgaggaa aaaagagcct tatagctctt tccccattgt agttctttaa tactgcttat
    17821 gatttgttta attccaacaa atttataaat ataggtaaac atgataacaa tagttattgt
    17881 gtaaatttgg aatatctgcc ctaataagac taaatagaaa gcaatcatgt tttcttttat
    17941 ctctttattc ttttttatat tgtaaataac atatgcactg tatactgcac taaagttaga
    18001 gattaaattt aaacttgcat taacatcatt gtttaaaagt tgtgaaaatg aaatatttgt
    18061 aaatgatgat gtcaattcta aatacactat atataaaaca ggtaatatta gaaaacaaac
    18121 cataaccact gaaagaaata tattttcatc ttttaatact gctgtgatat tttttatttt
    18181 atttgttatc atgtttttgt tctccttatt taaaagttgg ccagaaatcc ttattagcca
    18241 caatcatttc atctaagatt tcttttgcta cttttgcact tggaacagtt ttactcatag
    18301 taaacgactg ccataatttt tgataacttt tttctatcca tgcttctaca gctaattttt
    18361 cagaagcaac ttgttgttcc attaaacctt tttggaaagt tggaatttta cctatagaca
    18421 taggttcaac catatctttt ccaacaaggc aaggaatttc aaccattgca tcatctgcaa
    18481 aattttcaat agctccatta ttaggaacaa tcagtaaaaa tctttcctga gtattaaatg
    18541 ctaatgcaca agctaattca acaataaatt gagaatgttc acttgcatca aaccatgtat
    18601 ctcttgctgt gccatttttt ataattcttt cacattcttc gaatgtatcc ttttctcttc
    18661 tatccataat ttcatttgct cttgtataat ttggattagc atgttcaaca gtttcatcag
    18721 ccattaaata atattttaaa taagtattag gtaatgtagt tggttctatt tccaataaat
    18781 cctttgctgc tctcatagtt tctaaccaac tagattcttt gtgttgacct tgaggtgtaa
    18841 attcctcata tcctttttta gcacaatatt cctgtaattt aggcattaaa tcatttcctt
    18901 ctttgtcctt aatacttgtc caccacccga aatgattcaa tccataatat ctaacatcaa
    18961 agtctttacg agaatttaaa cctaaaactc tagcaaatat ttcctctaaa catactggca
    19021 tatcacaaat attaataatt cttgaatttg gtcttaatac acgacatgct tctgcaacta
    19081 tagctgctgg atttgaataa ttaagcatcc aagcatttgg agaatatttt tccatataat
    19141 caatcatttc gattattgga cctattgttc ttaatccata ggcaattcca cctgccccac
    19201 aagtttcttg accaacaacc ccatatttta gtggaatttt ttcatcttgt tcacgtaaag
    19261 catataaacc aacacgaatt tgtgccatta caaaatcaat atcagtaaat gcttcttctg
    19321 gatttgttgt ataactaaat ttaatttctg gatttttctc tctaacaatt attgcacatg
    19381 cttcagcaac ttttctttgt ctatcatcat tattatcata taatttaatt gattcaatag
    19441 gaaatttagc aacactttca attaacattg ttacaattcc agcagtataa gtactacccc
    19501 caccagcaat tagtatcctc tgtctttttc tttccatctt tccttctcct ttataataaa
    19561 ttttcaaatt cttctcttac acttggtact gaaagcccaa taataatctg aatagctttt
    19621 ccattatgca ctaaaccatg tgcacctact gccttaaaat cttcttcttt agaaataatt
    19681 gagtcatctt tcaaggttaa tctcaacctc gtagcacagt ttgtaacatc tacaacattt
    19741 tctgctccac caactaattt tatgtattca gatgcttgtt gagaggcttt actcaattta
    19801 tttccttttt catctttagt ttctaagtat tcatttttag tatatagttt tgtttcttca
    19861 ctatcttcct ctctaccagg agttttcata tcaaacttct taattaagaa agtaaataca
    19921 aagaaccaga taaatgaaaa tataatccct acaacaactt gaacaacata taccttccag
    19981 tgatacattc caagaggaag ccaatttaat acaacccagt taataattcc tccaccaaaa
    20041 tcaccaacaa ctcctagggc aaacgaaatc gtttgcaagc atgcatctaa aaatgcatgt
    20101 actaggaata aaagtggtgc tacaaataaa aatgtaaatt ctaacggctc agtaattcca
    20161 gttaatactg cagtaagagt agcaggaatc attaaaccta atactttttt ctttttttct
    20221 ggctttgcag ttacatagaa agcaccaaat acacctaatg aaccaaacac cttcgataat
    20281 ccggataatg aaaatccgat tggatacatt tcctttagag tcttagctga agtttgaaaa
    20341 tcctgaatat gagtagccca atatactgat gtaccacctg gaacaacaac actatcataa
    20401 caaattggtg catatacaaa atgatgtaat ccagttggta ttaaaatctt ttgtaagaat
    20461 gcataacacc aaactcctat atttccacta tttataaaaa acgtttgcaa atgtctaata
    20521 acattttgaa tatgaggcca aataaaacaa gttaataatg caactggtat cataacaaag
    20581 aaacaaattg caactacaag tgatgagcct ctaaataatc caataaaatc tggcagttct
    20641 ttatcataaa atctattatg aatataaatg acaatacctg atattaacaa agcaccaacc
    20701 ataccagtat ctaatgtttt aataccagca atgcttacaa gtccacttcc agcacttatt
    20761 tcagctgcga aatcaactcc aaagcttttt ccccaaaaac ttaacataga acctacaaaa
    20821 taattaaaag tcaaatacgt tgctaaagcc tccatacaag ctcttgcttg ttgctttttt
    20881 gcaagtgcta ttggtaaaga aatagcaaat aataatggca attgattaaa taccatccaa
    20941 gctccactag atattacacc ccaaaattgg taccaaatat tttctggtgt agcaagtgaa
    21001 cccataattg actgattttg aaatacaatt gagaatccaa ccattatccc agcaaaagca
    21061 aataatagtg ttggtgtaaa cattgcccct ccaaaacgct gtattttctt catcatataa
    21121 attactcccc tcctcatatt tttagtttat ctttggcctt agatatctat tattttaaca
    21181 ggttaataaa tatatacaat ctttagggta cattcggagc ttattcacag tcaaataagt
    21241 ttttgcacat ttttatgttc aaattctcat aaaaaaaaag aatgactaca atttgcaatc
    21301 aatcccttct gtaatcattc tatgcctttt ataaatacta tatttaataa ataataactc
    21361 aatcaatata aaatataaca ttgtcaattt ataactaggg tgcatttgta aaaatgagat
    21421 attagtcgct gaaatataaa ttgattcatc acttaaatca gtcaatgtat tatttttaaa
    21481 ctctgtaatt gaaattattt tagttcctat taattttaat cttctagcaa tattaagtgt
    21541 attctcattc tcaccactta gggatataat aatcataaca tcatttttac tgacaatatt
    21601 gtaaaaagaa tcatcaaatg ttcttccctc aaaatcatat attatttctt gacatgataa
    21661 gaacattctc tttatctgtt ttgcaactac attttgaata tcacctgaac cataagcgta
    21721 tattgttctt gattcataaa gaagtttaca caatgcatca taatctttat ttttttgatc
    21781 ttcaacatac ttaattattg aattacatgc aatatcaaat gactgatttt ctatgacatg
    21841 aaccttttct gattcccagc ttaataatac tttaaactca ctgaaacctt ttaaatctaa
    21901 ttttttaaca aatcttgtaa tagttgcacg agacacatta catttcatag caagttcatt
    21961 tattgacatc ttactacaat gttttttatt tgcagttata tatttccata ttaataaatc
    22021 tgtatcattc aatttattat aatttaaaaa aatcatttct tctaatctca taaactatta
    22081 ccccctttaa ttaaaaatcg tcataaatat tataccaata ttcaacatta aattacagcg
    22141 tattcttata tttactattt agcccataaa attgactata aaaaatactg ctataattga
    22201 tgtttaaaaa aatgtagttt taattttatt cttttatcta ataattaata ttttcaagtt
    22261 ctagtgcttt tcttatacaa ttatcatctt taaaatcagc agtcctttta caatctttta
    22321 ctttaaattc tggaatagtt ttaaattcct catcacatat tccattctta tttaaataca
    22381 tacaacttgc cattctaact attagaccac tattttttaa attaaacaat acagggtcta
    22441 tacaccctcc atctccgcca gtagtttcac caactaaagt cgcaaattta gtttctttac
    22501 aaaacattgc aaatgactct gatgatgaat atatttttct atcaacaagt aagtatatat
    22561 tacccttaaa ttttgaatca tctgtagatt ttatagtata ggaagtatct tcaaaatcat
    22621 taaaatcttt tataatttct ttaggtgcat tttcctttac acttgttggt aaattttcta
    22681 caggatttaa ttttatattt cttgcatttg tatattgttt tactatttca ttattatttc
    22741 tataaattct ataaccattt attttgacat cattttttat gagtttagat acgattccct
    22801 gccaataact atcacttcca cctaaattac ctcttatatc aataattaaa gctttatgtt
    22861 tttctaaagt gtcaatataa tctccaatta tttttaaatc attatttata gagccattct
    22921 ttgatgacat tgatggtaaa tacatatatc caatctgacc cttaataaca tcctttaaaa
    22981 ttaattcttt ttttacaaaa atatcatctg gcatttttat tttaagattc atagagtcat
    23041 atctatctac aactttttta tcattaagaa aatcatacca atcattatta gagtatgatt
    23101 tcttaaaaag ttcatatctt ttcttattat ctattacttc tgtgtgttta ttatttaaat
    23161 cagataatat agatgacatt tcttctataa attcttcatc attttcagtg ttttttattc
    23221 gttttgtata ttcatcctta tttgctaacc aatcaatatt attaagcctc ttatttacac
    23281 ctagatatgg atatcctgtc tttacaacat tatacacata attaaagtca tccacctttt
    23341 cctcttcaga caactgattc ttttgagaat aagtagaatt acctaataag attataaatg
    23401 ctaaaataat tactaaaaat aatactttct ttttcatgta attctcatcc actttcagat
    23461 tttatatttt aatacattgt atcattattt tattatcacc taaacagtta aaagaaattc
    23521 ttgaagatat attccactat tattctcttt aaaaattttt atttgtattt tcaaaattat
    23581 ttcatgaaag ttataaaata acaattatat tcaaatatct attttactgc tatatcctga
    23641 aggtaaattc ttatcagtga tatactaaaa aagatatgct aaaaaattta tccattataa
    23701 tgacttaaat ttagatactt tactgatttt tatttattag ttgagaaaga tattgataat
    23761 taaaaatatt gataactata gaaatgatta ttttttatag aaattttaag gatataaact
    23821 aattatactt aagtctatat ccttaattta tcatgattat gagaattttt acaaatactt
    23881 atctttgtcc gttttagaat aaaaacaaga acatatcctg ttattttatt caaacatcca
    23941 aatttaatta ctacacaatt acttattaga aattgtatct acttcatata tatatctgtt
    24001 aagtatagct tctagcattt cttgtcttcc agacttattc tttataactg gcatttgcag
    24061 tgcataagat tctaactctt taaatccaac cttaccttct actatatcct tacctatacc
    24121 ttcactaaaa cttgcatatc tttctttagt aaagttttca aatacctcat cttctaataa
    24181 tttatgagct attaataatc cctttgcaaa tgtatccatt cctgcaatat aagctaggaa
    24241 taagtcttct acctcaaaag atgctcttct tactttagag tcaaagttaa atcctcctgg
    24301 tgctattcca ccttgcttta acacttcata catagcaagt gttgcatcat atatatttgt
    24361 tggaaattgg tctgtatccc aacctaataa taggtctcct tgatttgcat ctatactacc
    24421 taaaacattg tttattcttg ctatatttaa ttcatgttgg aaagtatgtc ctgcaagtgt
    24481 tgcatggttt gcttctatat tcattttgaa gtatttatcc agattatact ttcttaaaaa
    24541 tcctaaaaca gtagcagtat caaaatcgta ttgatgttta gtaggctctt ttggcttagg
    24601 ctctattaag aattggccag tgaatcctat ttccttagca taatctacag ccatttgtaa
    24661 gaatcttgca aagttatcca tttcaagttc tgtattagtg ttaagtaaag tttcataacc
    24721 ttctcttcca ccccagaata caaaattttc tcctcctaag tacttagtta tttccatagc
    24781 ttttttaacc tgagctgctg catatgcata tacatctgca ttacatgttg tagatgcacc
    24841 atgaacaaat cttggatttc caaacatatt ggcagttccc cataataact ttatacctgt
    24901 cttttccata ttaactttta tatactcaac tatttcatct aatatcttat ttgtctcttc
    24961 taaatctctt ccttctggtg ctatatctct gtcatgaaaa cagaaatact caattcccat
    25021 cttagacata aattcaaatc ctgcatcaac tttaatcttt gctatctcaa ttgggtctgt
    25081 tatattattc caaggtctct ctactgtgcc tactccaaat gggtcattac cctctccaca
    25141 taaagtatgc caatagctca ttgcaaatct taagtgttct ttcatagtct tattacctac
    25201 cacttgttca ggattgtagt atctaaatgc taattcattg tcagatttta ctccttcaaa
    25261 ttttatctgt cctattcctt taaatatttc actcatttca tgtcctccta atattttaca
    25321 catataatat atgtagtatg ttttcttaat ttaaaactat tttctatctt tatttttctt
    25381 tttaagctaa tttaattatt tatttttgca ttaactactt catctaataa cttaaagtca
    25441 tctttcaaag atttgtatat tcttttatac aatctatgat atatattgta tgtatctaca
    25501 ttttcactta ttggttctaa tttagttgaa gtttttatta tagcatcaca tgctttttgt
    25561 atattttcat atattccagc tcctactaat gcaagaattg caactccaag tgctggtcct
    25621 tcacttgaat ttatagtata tacatcttga ttaaacatat ctgcttgcat ctgtctccat
    25681 actttacttt ttccacctcc acctgaggct cttacttcat taacgtctat attaagattc
    25741 tctattatat ccatacagtc tttaagacta tatgaaacac cttccattat tgaccttgca
    25801 atttcttttt tagaatggat tggagataat ccaaagaaca cacctcttgc ataagggtca
    25861 agatgaggag ttctttctcc catcatatat ggtaagtaaa gcaatccatt acatcctgtt
    25921 ggtacttgac ttacttgatt atttataatc tcatatacat cttcacctaa agaatttgaa
    25981 acttccactt cactttgaca gaaattatct ttaaaccatt ttaatgacaa gccagctcct
    26041 tgagtaactc ccataacatg ccatgtattt ggtattgcat gacagaaagt atgaactcta
    26101 ccttctttat caatttttgg ctcttttgta taagcaaata ctacacctga tgtacctata
    26161 gtagatgaaa ctactccatc tttaactatt ccatttccta tagctcctgc tgcttggtct
    26221 ccagctcctc ctaccactat tgttccttct ttaagccctg tcaaacttgc tacatctcta
    26281 gtcacataac ctgtaatctc ttgtgattca taaactttac ctaaaagatt tttatctata
    26341 tttaactttt ctagtacttc atcgctccaa tctctttttc ttatatcgag caactgcata
    26401 ccacttgcat ctgatacctc tgtagcatat tcacctgtca atttgtatct tatataatct
    26461 tttggaagaa gtattttatt agtattttta tatatatctg gttcattgtt tctaacccat
    26521 aatatctttg atgctgtaaa tcctgtaagt gctggattag cagttatttc aatcagtctt
    26581 tcttcaccta ctaaatcagt tatctcctca cactctttta ctgttcgttg gtcacaccat
    26641 attatagagt ttctcaatgt tttaccttca ttgtccagca taactagacc atgcatctgt
    26701 cctgataatc caattccttt tatacattca ctgcttatat ttgaactttg aactacttct
    26761 cttatagatt cgactacagc tttccaccaa tcatttgcat cctgctctgc ccaacctgat
    26821 ttctcttgta taagaggata ttcatatgta caacttttta tagtatttcc tagtttatca
    26881 aataaaactg ttttagtacc tgatgtacca atatccacac ctaatacata ctccaacttc
    26941 atccctcctt gaatgccact tcataactat cgttttccta tcctaatttt tttatatttt
    27001 aggtatattt tacttgcttt atttttgtta gtatattaaa ctaacttatg aactaataat
    27061 aaaatattta attatctttg tcaatgcaaa ataacctttt atttttgtgt aaaaagtatt
    27121 acaatattat tttaaggagg aatatcaaat tggttactga taaatacact attcgagaaa
    27181 tgaatgaaag attggtactt gaacaaatta taaaaaatgg tcctatttct agggcaagta
    27241 tagcttctac tataggattg aataaagcaa ctatatccgc tattaccaaa aaacttattg
    27301 atgaatctct agttcatgaa atcggaattg gaaatagtac tcatagtggc ggaagaaaac
    27361 ccatattatt agtatttaat aaatgtgcag ggatttcttt aagtatggac attggatacg
    27421 actatatatt ttcttctcta tcttacttag atggaactat tataaattct aagaaattaa
    27481 cagatataca agtaagtaaa gacaatgtta tacaattgat agatgaaatt ataaatagtt
    27541 ataacatatc caaaatagac actccttata aggtaattgg tcttactttg gctatacatg
    27601 ggattacttg tgaaaataaa gtattgttta ctccatatta caatcttaat gagattgact
    27661 tatatagcat ccttagcaaa aagtatgact ttcctattca tatagaaaat gaagcaaact
    27721 taacagcttt agctgagaat actttttcaa ctgtacataa ctcactactt agtctcagta
    27781 ttcacagtgg atttggttca ggcattataa taaataataa attatatagt ggcagaaatg
    27841 gtatgagtgg agaaattggt cacactatca tcatgcctaa cggtaaacta tgtccttgtg
    27901 gaaatcgtgg ctgtttagaa cagtattgtt cagaaaaaaa ggtatttgaa cagctttctt
    27961 ctttagaaaa tattcctaaa atagattctg atatagtaaa gcaactatat tatgaagata
    28021 accataatgc caaaaaagtt attcatgagt tttgtagtta cttaacaata gctataaata
    28081 atgctattac aacttatgct cctgaaataa tatatttaaa tagccaaata attagtgata
    28141 tacctgagat attacagatt acaaaagata tgttagttag ctcttttaac aaaggaataa
    28201 atatagaaat ttcatcatta gggtctgagg catctctata tggtggctct gctgtaaata
    28261 tcaagagctt tttgaatata caaaatttaa ctttaattaa tgaaatataa gggttatgac
    28321 tatatacagc cataaccctt taaattatac attctaaaat tagatttatt atatagttta
    28381 tgcaagatta gctatatttt ttaaaaattg tatatcaatt aagtattaac ataagtactt
    28441 cgttttaaat attaatataa tcttcctact catcatcaga taataaactt gcattttgtt
    28501 caaccataga aatattatct ttacctattt tctctaattt tgctgccaat tcctttgtat
    28561 ctaaaaaagc tgattcaata accataccta aatttagccc tgaaataatt ttaattcgac
    28621 tgtcattatt ggctactaat aaagcagcat tgcatggagt tccacctagt aaatctgcca
    28681 ttataattat ttctgaactt tccttgtaca cttctaaagc tttttttaat ttttctattg
    28741 ttccttctat tccatcatct tgtttcatat ttacagtagg ataatctata gaatccccta
    28801 ttatcatttt tgcagaattt aagacttctt cagccatatt gccatgactc atcaaaataa
    28861 tactattcaa tttaatacac ctctttaaat ctataagatg tctacaagat aagttttctt
    28921 atcatcaggc gtcattatag ttgtaatttc aattccttca tctaaaagtc ttttaaatgc
    28981 ttctttctct tcttcagtaa cactgatatt aggtcttaga actgttgtat cttttctact
    29041 tgacatattt cctacattta tattatgtat tttaaatcct gcctctacaa atggaataag
    29101 gtccacagga gacttaacaa ttaataatac attttgtcct tcatactttc ctgcagtaat
    29161 attttttata gcagtctctt ctgtaattat agatgacgct atacctgcag gtgtagccat
    29221 tcttaaaact tgtttttgaa cagaattgtt agctactgca tcatttacta ccattaatct
    29281 tgtaacaccc aatttattac tccacatagt tgcaacttga ccatggatta aacggtcatc
    29341 tatacgaata tttttaatac ctttcatttc catatctaat atcatccttt ccttatttta
    29401 aaatacctaa tgcatataaa attattgaaa caaccattac tcctaaaatt aagtatatag
    29461 gttttatatt ctttttaaga gcccaataaa caatcacaac tagaattgca ggaactaacc
    29521 caggcataat ttggtctaat atttcttgac ctttcattgt aaaatcacct tgttgaaagt
    29581 tcaaagcaac attagctttt acaactgaag gtattaatgc acctacaacc attaaaccta
    29641 aaatagatgt actctcagtt aaaatcttta aacttttttc aattgaagat attagcttag
    29701 tacctgatgt ataaccagca ttaaatagtg gtatacttaa taatttcaat gctacactca
    29761 ctacaatcca cagtaagatt ccaaatggac ttccatctat cgccatatta gctgctatag
    29821 ctccaaaaat tgtccacgga ataacgacaa ataaactatc tcctaaacct gctactggtc
    29881 ccatcaagcc tgttttaatt gaagcaacag cctcttcagt ctcttcactt gataactcct
    29941 ccattgctac attaataccc aaaatataag gggcaaccca aggattagtg ttaaaaaatt
    30001 gtaaatgatt taatacagca ctttgtaact tttctggatt atctttgtat actactttta
    30061 aaaaaggtag tactgcataa cagtatgcca aggcctgcat cttctcataa tttgaagaaa
    30121 cagaactagt aaaaaaccag cgccataaga cacttttttt caccttttca tttgatttat
    30181 ttaatgatac cttatcactc atcttcgtcc atacctccta ctacagtata ttgttgttct
    30241 tgattttcca aattcttttt gtaaattata agtgcaattg ctccaccaat taaagctaca
    30301 cctaataatg gtactttcat atatactgct aaaacaaaac caattactaa gtaactaaaa
    30361 tactccttag caggaagata tctaagtaat aatcctatcc ctactgctgg aagtagtcct
    30421 ccagctactg ttaaaccacc tgttaaccat tcaggtgtat atttcaaaat ggtattaatt
    30481 aaagaaggtc caaatattac agatagagca actggaatac ctgttgtagc tgctgttaat
    30541 agtactccta acatttgcat ataggttata tttttataat ttcctttttt agctccatct
    30601 tctgctttat gaactagcca tataccaata gtatttctaa gaacatctaa ttgtaccatc
    30661 aataaagcaa ctggaatacc tatagagatt cctacagatg cttcttgctt agtcgtaata
    30721 gttataaatg tggctactat acttgctgtt tgataatcag gtattgatgc tccaccaaag
    30781 ttactaatac ctaatgataa tagctgtaat gtacctccaa tatataaccc tgtatttata
    30841 tctcctaaga taagaccagt aataaaacct gctgttactg gttgatatgt tccaaacatt
    30901 gttgaatttt tttcatagtt tataaaaaat ccatatagta cgattaataa taattgccat
    30961 aattgtggct ccattatgat ttcacctctc ccaaaatatt ttttagttct ttaaaattat
    31021 aactggaatc ttgctttagg aaaataggaa tttcttccag tgggcattca acttcataag
    31081 ttttcccacc ttcataacta actccactaa atatattttc ccatgtatgt ccttttggta
    31141 aatatacttt tctacttcta tctttataat tcattattgg agctaccaaa atttcatctc
    31201 caagcatata tgtattatcc acctcccaag ttttatcatc ttcaggaaat tcataaaata
    31261 aagttctcat aactggtgtt ccatcttcat gcgcttcttt catcagtttt tttaaataag
    31321 tttttaagct ctctctaatt tttatgaatt ttgtaagaat tacttcaact tcttcaccat
    31381 aagaccatat ttcattagga gctccagtta ccattgagcc tccaccttta tttgataatg
    31441 gtttactatg aggcaatcta tctccatgca ttctcaaaat tggtgaaaaa gtagcatatt
    31501 ggaaccatct taccataagt tctctaaatt ctgggtcttt aggatttcct ccatggaaac
    31561 ctccaatatc agtagtccac catggtatac ctgccagtcc catatttaac cctgtattta
    31621 cttgattatt aaaagcttca aaactagaat caatgtcacc agaccatact aaagctccat
    31681 acttttgagc tcctgcccaa gctcctctta caagagttac aacagactct atgtcattat
    31741 gtaaaccatc ataaatcatt tttaaataat caataggata aatattacca caacttaaaa
    31801 catttccttt tttatatcta tagttatcaa aatcatacac agaatagcca ggttcagcta
    31861 catctaacca ataatagtca atcccaagtt gtttataatt tttatctatt ctatcccaaa
    31921 catattctct tgcattttca tttgtcatat ctacaaagac tgtatttcct tgaatttgca
    31981 ttgtcattct aacaccacga ttaacattga ctaaatagcc attttctaaa taatcattgt
    32041 agttttctgc atcactttga actgtaggcc atacagatac tattggttct actgaatact
    32101 cttctttcaa ttttttaact aattctttag gatttttcca ataatctaag tcaaatttat
    32161 attcaccttg cttaggccaa tgaaaatagt caattgcaat tgatgaaagc ttaattcctc
    32221 ttttagaata ttccttcaca acatctaaaa cttcttctga agttctatat cttagtttac
    32281 tttgccatag tcctaaaagg ttttcaggca tcataggaac agttccagta acttgagaat
    32341 aatttttatt taactctttt gggctttctc cacaagtaat ccaataatct ataaaatttg
    32401 tagaaaacat cttccattct gtcatatttt tagcaaaaga tacttttcca attcccggat
    32461 tattccataa aagtccataa cctagactag aaatataaaa aggaactgat atttgagaat
    32521 ttctttgggc taactccaaa acagtattct ttaaatctaa aaatttatgt tgatattgtc
    32581 ccattccaaa aatcttttca gaaggctcag attcaaatct agtagttact tcaaattctc
    32641 cactatagct tggtcgatat tctcttgatt ttaactttag agtagagtta aagtctttgg
    32701 taatctcaat tgtccctaca tcttctccac catcatcatg ttttacagct cttaatcgaa
    32761 tatattcttt caataatatt tcatttttat cattataaaa agtaattcta tctctatggt
    32821 ctaagacagc ttttatcttt ccatttttaa tgattgcact tccatcttca tttttattaa
    32881 ttgtaatatc atctttattt aacttaggtt tttcgttaag agcatagttt tcatcaacaa
    32941 aattttggtc tacaaacgac ctaatacgta aagagttttt tccccatgct tctattcgca
    33001 atagttcatt gtcaaatctt ttttcaatat agtcttcaga tatttttatc acggctacct
    33061 cctaaatcaa tggtataata ttctatcttt gtgtagctta agttaaataa tgtcgtttag
    33121 tatcgttatt tataaattag gttttatata taattcattt gtacattaaa ctaacttacc
    33181 aataatatac tttatttgac tttgtttgtc aatcgctttc cggattttag taaaattttc
    33241 taatatattg ctggaaagtc gttcttaata tcttttattt atacttttaa tatctgtgat
    33301 gatacctata tacatctagt ttttttaaaa cacatacagg aataacattt gcctattatg
    33361 tagataatat cggacttgaa aatatgtata gtgaccttat aaatacagat aataatatat
    33421 tagatattgt tcaaaacaat ggattttata attataaata atttattaaa ttatttaaga
    33481 aaatatacaa ttgtatacca agtcaaaaac gacaacaaat aaataaaact aaaaaagatg
    33541 ttgtctcaaa atatagataa atttattttt cattgctaaa atgattaaaa tatatttgat
    33601 ttaatatact tgatttaata taaaaaaaga gtgtctcata aactaaaaag ttcactttta
    33661 gacactcctt tatatatatt atctacttct taattcattt attacctctc tacattttct
    33721 ttcaactagt ggatacatct ttataagtac taattactcc acttaaaaat gattcaggta
    33781 ctgtccatca gtcatatttt tctgccaaat ataatggtgt ttcataatga gacaataaat
    33841 atttatatct ctacttatct tctctaagct cgtcaaatcc aactataggt atatttaaat
    33901 tatattcttt aagtagcttt tctagctctt ctataaaaat acctttccca tccaccattt
    33961 gtatatcatt tattttatcc atcaattcat tgcaaccact taactcacca aaccaacgtt
    34021 tagaactaca tgtcttatta taaccacaac ttggactgcc atctattgca atgatacttt
    34081 ttatatcata atcatttttt aaatattcat caaactgcct tacatgaggt attaacattt
    34141 gcctacattg ttctctaaaa aacaaattgt caaactgttc ttttacatgt ccccacctat
    34201 ttattccata cataatcatt tctgggcatg gtagttgaat tattccatac cctttattta
    34261 ttaaatctat cgctatattt tgtgctccct taaattctga caatccctct actttagaat
    34321 tacaatttaa tatacaatga catactacta caatctctct atttctcttc ataataatct
    34381 ccttagttat tcttaatttt aatgatacta catattttga taattatatt atttatttgt
    34441 tctatatttc caatattcta tatagaaata tacaattagt aatcttttat aatctatact
    34501 atgataaatg tatatatttt tttaggaggt attataattg gatactaaaa aagtcacatt
    34561 ttcttcaatg tgcatagtta ttaatattgt cttaggtatg gttatatcta tgataaatat
    34621 accattatta tttttagata caattggtac tgtacttggg gctgtcgtat taggtccttt
    34681 ttggagtgct cttattggtg ggtgtacaaa cttagtttta ggtgttatat caggtcctac
    34741 aaatatacct tttgcccttg taaatattgt tcttggtctt attgttggat atatttctaa
    34801 gaaaaaaggt tttggttata aagaagctat tattacagga gtaattttat ctattgtttg
    34861 tcctttaatt ggtactccta tttcagtttt attgtttggt ggtttaagtg gaagtggtgc
    34921 tgatttattg gttggttttt tagttcagtc tggtcagaag atatttactg ctgcgttctt
    34981 tccaaggatt cttagtaata tagtagacaa acctttgtct tgtattatgg tggtatattt
    35041 tataagtaaa atgcctaaag gttttataaa acaatatagt aaaaatactt ctagttcata
    35101 ttaataaatc atttattatg taagatttat tactctttaa aatgctatat actcattttt
    35161 atataacatt tttattaata atttaattag at
//
LOCUS       DAMKMI010000024        35082 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMKMI010000024 DAMKMI010000000
VERSION     DAMKMI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35082
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.025"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            38..214
                     /locus_tag="PC429_001133"
     CDS             38..214
                     /locus_tag="PC429_001133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846648.1"
                     /translation="MNLKKKEKIVKPVLKKLTEIVLNILSKKILNYAIYAISVLIVTK
                     VVINIDLYISILYY"
     gene            315..1052
                     /gene="cas6"
                     /locus_tag="PC429_001134"
     CDS             315..1052
                     /gene="cas6"
                     /locus_tag="PC429_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF3846649.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            1057..2532
                     /locus_tag="PC429_001135"
     CDS             1057..2532
                     /locus_tag="PC429_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846650.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMEKNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2552..3568
                     /gene="cas7i"
                     /locus_tag="PC429_001136"
     CDS             2552..3568
                     /gene="cas7i"
                     /locus_tag="PC429_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF3846651.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3584..4381
                     /gene="cas5"
                     /locus_tag="PC429_001137"
     CDS             3584..4381
                     /gene="cas5"
                     /locus_tag="PC429_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF3846652.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4408..6816
                     /locus_tag="PC429_001138"
     CDS             4408..6816
                     /locus_tag="PC429_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF3846653.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7258..8205
                     /gene="prmA"
                     /locus_tag="PC429_001139"
     CDS             7258..8205
                     /gene="prmA"
                     /locus_tag="PC429_001139"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF3846654.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            8224..8982
                     /locus_tag="PC429_001140"
     CDS             8224..8982
                     /locus_tag="PC429_001140"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF3846655.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8984..10282
                     /gene="mtaB"
                     /locus_tag="PC429_001141"
     CDS             8984..10282
                     /gene="mtaB"
                     /locus_tag="PC429_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF3846656.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10325..10675
                     /locus_tag="PC429_001142"
     CDS             10325..10675
                     /locus_tag="PC429_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF3846657.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10838..11017
                     /locus_tag="PC429_001143"
     CDS             10838..11017
                     /locus_tag="PC429_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF3846658.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            11047..11490
                     /locus_tag="PC429_001144"
     CDS             11047..11490
                     /locus_tag="PC429_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF3846659.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11601..12098
                     /locus_tag="PC429_001145"
     CDS             11601..12098
                     /locus_tag="PC429_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF3846660.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12291..12854
                     /gene="raiA"
                     /locus_tag="PC429_001146"
     CDS             12291..12854
                     /gene="raiA"
                     /locus_tag="PC429_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF3846661.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            13067..13303
                     /locus_tag="PC429_001147"
     CDS             13067..13303
                     /locus_tag="PC429_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF3846662.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13316..14659
                     /gene="yqfD"
                     /locus_tag="PC429_001148"
     CDS             13316..14659
                     /gene="yqfD"
                     /locus_tag="PC429_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF3846663.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14598..15713
                     /locus_tag="PC429_001149"
     CDS             14598..15713
                     /locus_tag="PC429_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF3846664.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15770..16231
                     /gene="ybeY"
                     /locus_tag="PC429_001150"
     CDS             15770..16231
                     /gene="ybeY"
                     /locus_tag="PC429_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF3846665.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16237..16956
                     /locus_tag="PC429_001151"
     CDS             16237..16956
                     /locus_tag="PC429_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF3846666.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            17075..17476
                     /locus_tag="PC429_001152"
     CDS             17075..17476
                     /locus_tag="PC429_001152"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF3846667.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17540..18433
                     /gene="era"
                     /locus_tag="PC429_001153"
     CDS             17540..18433
                     /gene="era"
                     /locus_tag="PC429_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF3846668.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18445..19824
                     /gene="mgtE"
                     /locus_tag="PC429_001154"
     CDS             18445..19824
                     /gene="mgtE"
                     /locus_tag="PC429_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF3846669.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19897..20052
                     /locus_tag="PC429_001155"
     CDS             19897..20052
                     /locus_tag="PC429_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF3846670.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            20018..20791
                     /gene="recO"
                     /locus_tag="PC429_001156"
     CDS             20018..20791
                     /gene="recO"
                     /locus_tag="PC429_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF3846671.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20784..21383
                     /locus_tag="PC429_001157"
     CDS             20784..21383
                     /locus_tag="PC429_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF3846672.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21543..22421
                     /gene="glyQ"
                     /locus_tag="PC429_001158"
     CDS             21543..22421
                     /gene="glyQ"
                     /locus_tag="PC429_001158"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF3846673.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22421..24487
                     /locus_tag="PC429_001159"
     CDS             22421..24487
                     /locus_tag="PC429_001159"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF3846674.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(24653..26200)
                     /locus_tag="PC429_001160"
     CDS             complement(24653..26200)
                     /locus_tag="PC429_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF3846675.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26480..27247
                     /locus_tag="PC429_001161"
     CDS             26480..27247
                     /locus_tag="PC429_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF3846676.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27530..27727
                     /locus_tag="PC429_001162"
     CDS             27530..27727
                     /locus_tag="PC429_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3846677.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27756..28832
                     /gene="vorB"
                     /locus_tag="PC429_001163"
     CDS             27756..28832
                     /gene="vorB"
                     /locus_tag="PC429_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF3846678.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28835..29554
                     /locus_tag="PC429_001164"
     CDS             28835..29554
                     /locus_tag="PC429_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF3846679.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29567..30106
                     /locus_tag="PC429_001165"
     CDS             29567..30106
                     /locus_tag="PC429_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF3846680.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            30263..31321
                     /gene="buk"
                     /locus_tag="PC429_001166"
     CDS             30263..31321
                     /gene="buk"
                     /locus_tag="PC429_001166"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF3846681.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31339..32232
                     /locus_tag="PC429_001167"
     CDS             31339..32232
                     /locus_tag="PC429_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF3846682.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            32262..33470
                     /locus_tag="PC429_001168"
     CDS             32262..33470
                     /locus_tag="PC429_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3846683.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33494..34753
                     /locus_tag="PC429_001169"
     CDS             33494..34753
                     /locus_tag="PC429_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3846684.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
BASE COUNT        14181 a         3549 c         6477 g        10875 t
ORIGIN      
        1 gtttaataaa tataaaaaga gaaagaggga attttaaatg aatttgaaga aaaaagagaa
       61 aatagttaag cctgttttaa agaagcttac agaaatagta ttaaatatat tatcaaaaaa
      121 aatattaaac tatgctatat atgcaatttc tgttttgata gtaacaaaag ttgttataaa
      181 tatagatttg tacatatcca tattgtatta ttagatatct tatagatata aaaaattaat
      241 agatatatat ctattaattt tttatattac aaatagagaa taatgaatta tactagaaag
      301 taggaggtga aaatatgagc agaatttcaa tttttatgga atcatgtaat ggcaaatttc
      361 caaatgaaaa ttcaatgttg tcagtttcct ttataaaaaa tattttaaat gcagaaaaca
      421 aagattttgc taaaaacata tttaactatg gggaagaaag aaaaaacaat aagcaaataa
      481 aggatataaa tacagccatt tacataccaa atcttataag aagcaaaaat gagttacttg
      541 ttgatggaga tataatattc aatataagtt tttataatta ttctatgttc agtaaattat
      601 acaatggaat tttaaaagta aaggagctag aatataaagg ttatagattt aaaataaaaa
      661 atataaaaat acataaagaa cacgagataa aagaaaatgg tgttatattt aaaactatgt
      721 ctcctataat agtaaagaat aaagaaggta aatatttaga tgtagaagat tcaaattata
      781 tagaatgttt aaactatata gctaatctaa ctctggagag tataagaggt agtgggctta
      841 gaaagccatt ggaatttata cctcttaact tcaaaaaaag agtgttaaaa gaaaagattc
      901 gtggatttaa agagagagaa ttttactata taaatgctta tgcaggaaca ttttttttaa
      961 aaggaaatat ggaagattta aatgcactgt ataaaatggg aattggatat agaaggacgg
     1021 aaaatgcagg aatggttgat atattaaagt agcgaggtga gagaaataaa agctaaattt
     1081 tatagatcag gtgatattgt aagagatatt ggcattgttt acttttgtga attactaatg
     1141 gatttaaaaa acgaactaga aaaaaatgat tataagctaa aatttacttg tgagttgaat
     1201 agaaattatt tatctttaga atctgcagaa tatatagacc ctaagtatat atctgattat
     1261 atattagaaa atcaactttt taaggtattt aaaaatggga aaaaggaaac cttagaaaaa
     1321 atatttagta atatagataa tttaacatat gtaaatttta ttgaagaatt ggataaatct
     1381 tctgcaactg agaaacaaaa ggaagggatt aaaaaagatt ttaaaaacag aagatttcca
     1441 tatgttagaa atagtggtaa gtatggatta aacacgagtc tagaaaatat ggaaaaaaat
     1501 gtaaaaagaa tagtcgatat tgtgattaaa tcaaatatag agatagatga aatagataaa
     1561 cttgatttga aacaatatga agaaaaagat agtgtatgta atgtttgcaa tataaataaa
     1621 acaacaaaac tagatattga tttaagagaa cgaaaggact ctaagtataa ctttttattt
     1681 agaggtgcag aaaaatcagg ttttaaaaga agtggacaag ttgaaagtaa tatttgtttt
     1741 gagtgtgaat ttttcaactt aatgtgtctt ttatatataa atttaaaaag accaatggtt
     1801 tttgcatata cgaatgattt aagagaatta gcatttttaa atcataagat aatgcttaaa
     1861 agaaaaatgt attcagataa atcattttat aaaaaacttt tgcatgaaaa aataagttca
     1921 attagactat ataggattga tattgatacg aataaaggta taattctaaa atttgactct
     1981 attatagagt ataaagaatt attaaaaaaa attgaactta tagatattat agataattat
     2041 aatttttcaa gagagccaat gaatactaga aatttgggta aaaaaatgat taataatggg
     2101 aatttatcaa atttaaaaga attattactt gataatataa gtgttttaag gcaactaact
     2161 ggaacttctc gagaaatgga ttttgtatct agttcatata atatcggttt atatataaaa
     2221 ttgtgttgga ttgtagatgg aagggaggag tataaaatga aaaactatat agaaagtaat
     2281 ttaatatatt caagagtagg taaagactta tttaataaaa tgacagatga atccagaaga
     2341 aaaaattttt ctatgaaagt gattcaacta cttaagtcta atgatagaac tcagctattt
     2401 caaactataa tgcatgtgtt agtttctaat ggaatcttaa taggagaagg gtttgtagaa
     2461 ggtctaatgc agagttctga attagaatta aatacgaatg taggtctatt tattcaagaa
     2521 atcatgaaat aaaaaagggg gaaaatttaa aatgaatgaa aaaagagtaa aaggagtaac
     2581 attaactata ctgacagaaa gcccagttcc actttcgtat gaccaagggt atggaaatta
     2641 tacaccaata aaaaaagaac aatatagaga taaagtacat gcaaaaacta gtatagctac
     2701 aataacatat gatttaagaa gaatactcca tcaagaatat ggttggaatt taagtaatat
     2761 tgtttttgga aaaaaaggaa atatatatcc atctattaag aaaaatgtag gatgtgtaga
     2821 tgaaaatgga ttagagactg atgtatttgg ttatttaata ccacttaaag agaaaggttc
     2881 tataagtaag gcttctccgc ttagaataat accttttagg tcactaaatt catatagagg
     2941 ttcaacacag ttgataacaa atagaggttt tttaagtagc gaatttggta gaaaatatta
     3001 tgatgagagg gaagaaaatg aagtacttag agatgaaaac tttccaacaa cacaagcatt
     3061 agcaatagaa gagactttat cagattacta cgtttataca ataacattgg agcttgatag
     3121 aataggagta gtagaagtag aagaagatgg aaagttactt ttaccagaag aaagaaaatt
     3181 tatgagtaag gagttaagag aaaaagcagt aaaagatata ttagatgcaa ttactgtgtt
     3241 tacaagaaac ataaaacatt catcagtact attaaaacca cttgcagtaa tgggtggagc
     3301 ttttgataaa gttgtaccat ttttttggga tgatgtagat tataatgctg atagtggtga
     3361 gataaattta gagggagtca tagaaactat agaaagttat agccttaaag aaagtaacac
     3421 aatacttgct atcaatgaca gattaaaaat ttctaataaa aaggaattaa ataagtttaa
     3481 tttaagtaaa taccctgtaa aagagattaa aaatctagca gatagattgg aaattggaga
     3541 agataatatg tggtatttaa aagagtagga ggttttttga attatgaaag tattacaatg
     3601 taaacttaag caaccaactg ctcactatag agacccaaaa gtatttcaaa atgaatatat
     3661 aagtacttta aatttaccat ctaagaccac aataatggga atgataacgt atttatgtga
     3721 tagaagatta aactctgata tagatatagg aataattgga acacatcatc atagagaact
     3781 agaattttca agaggagaga acattgattt ttggaatgaa tatacaaata tgagaaaagg
     3841 caaagataaa gaaaagtttt tgcttcaagg aaactactat gattactata aagaacataa
     3901 agctcaaaat agcatattaa actatgaagt tcttaaagaa gtagaactga ctatatttat
     3961 ttcttgcaag gatgatgaag aacttgaatt tattagaaaa aagcttgaaa gtccatgtaa
     4021 atatgcaaat ttaggaagaa aagaagattt tgtaatacca agtgaaaaag ggtgttttgt
     4081 gaaagaagtt gagttaaaag aggttatacc tatgaatact cgtgatgcta taaaagaaaa
     4141 tataaaatta aagaatacat atgtaagagt tgacttaaga gataaagaga atgtcgagac
     4201 tataataaat caaggtgtat tgattgcatt accttataaa tataaagatt tagaagcaaa
     4261 tagagatgat agagtttatg agttttgcca ttatatatat gttgataatg atggtatata
     4321 tcctaaaaat attaaggtaa atgtatgtat tgatacaaaa gaagttttta catggcttta
     4381 aattattttg aaagataggt gtttatcatg aaaaatatca tgcaaaaaga aatattagct
     4441 aagtcatata atttaagaac taatcaacaa gagcaaacat tagttgaaca tattaaggat
     4501 ttatttgaag tattagaatc tatattagaa ctaaatctat attcagataa agatgtagag
     4561 atactaaaaa tatgttgtgc tttgcacgat ttaggaaaaa ttaattcaat tatgcaacaa
     4621 aaaatggaga ttaataataa aatatcgtat agttgctcag aagaagagag gaaaaagtta
     4681 gagtctgaca aaaaatcttt aaaaaagata gcaagacata atatattttc tggagcattt
     4741 ttaaaagata tattagaaaa aatgaacttg tcagaagaag ataagttata tatatataaa
     4801 agtataatgt tacatcatgg aaactatgaa gattatatga ggttaagtat tagtaaagtt
     4861 caagaagaaa tttatgagta tattgaaaaa ggcattttag aaaaagaaga gtttaatctg
     4921 aaagatatag aaagttatat aaatagcatt ttaaatgtag actttaaatt tggtgaagat
     4981 gtattggatt atgattacat agataaatta agtgaaagta tgtttataga cagtgattat
     5041 aacaatggtc aaatagatga ttcagtttta aattatagaa agtttaagta tattttatat
     5101 aaagggatgc tgaacttaat agaccatagt gcatcaagta gacagaaagg tataaaattt
     5161 tataacgatt ttactgatga agaaattgat aatatgattt taaatgagat atataagagt
     5221 cagggtaatt tggaaaaaaa taatatagag ttcaatacta tacaaaaaag gttgagagaa
     5281 ctttctggaa gaaatgttct gactgttgca tttacaggtt caggaaaaac agctgcagat
     5341 tatagaaaga cttttaaaag aaaattcttt ttagtgccaa ataaaatatc agcagaaagt
     5401 ttttatagaa aaaatatttt tcaaaataaa aatgatttag atacaagaag tagtaatgat
     5461 tacattggat tacttcatgg agatataaac ttatattcag agaatgaaga taatggagaa
     5521 catgattttg tattgacatt aagggatata gatttgagta taaacttttg taaaccttgt
     5581 gtaattgcaa cagttgacca gttattactt agtatgttta aatttccggg atatgaaaaa
     5641 atattcgcag ccgtaaaaaa tgcatctata actgttgatg aagtacattt attggaacca
     5701 aagatgtttt tgattttgat atattttatg cagtttgcat gcaagtactt agatgttgat
     5761 tttcacttaa tgacagctac acttccaaaa tcatataaag agcaaatgat aaacaaggga
     5821 attatttttc aagaagaaag taatgaaaat gttactgata aaggagaaat agtttttatt
     5881 gagagcaaca aggaagaaga gatttgtgaa ggtaaagatg taaaggtttc ttttataaag
     5941 gaaaaagaaa tcaagtcaat agttgaagat gctttagaaa ataaacaaaa aattcttata
     6001 ataaagaata ctattgattc agtcaataga acatatgagt ttttaaaaga aaatttatct
     6061 gataagtatg gtggtgtaga tatagatgtt ttacatagta gatttaagtt caaagataaa
     6121 aaagagaaat atagtaaaat attaaatgga gagggtgata tttggatatc aactcaatca
     6181 gtagaaatat ccttggattt ggatttcaat ataataataa gtgatttagc aactatggat
     6241 agcttgatac aaagaatggg tagatgtaat agaaataata aatatgaata tggaaacttt
     6301 tatattctac ctagtgaaga taaaatttat gataacaaac ttaagaacac aactaaaaac
     6361 atcttaaagg acatattaaa aactaactct atttttacaa tgggaattag aaaaactatt
     6421 ttggatgatt actatgataa tagtgtagtc aaaaaatatt ttgaagagaa ttttataagt
     6481 tgtgataaag aaattaagaa tatttatggt ataaataaag aaatttttga tggattggat
     6541 ttaattttta attttgaacc ttacaaaaat attgttgata gtaagaagga agcagctaaa
     6601 atttttagag atgttgatgt tagttacaaa attattctgg aagaagattt ttataaggaa
     6661 gatagagagt tacaacaaga ttcgattcaa gtatcagggt ttatatttaa tagattatat
     6721 tattatgggt taataagcaa agtagaagga tacatggtac taaaatctag tagtaagttt
     6781 gagtataata gtactgtagg cttaattttg aagtgagcat atgtttaaat ataatatacc
     6841 tttagacatt gaaaatacaa aaagtactac tgtaagctca ctgtaaaaac tattttttta
     6901 aaaggaaaaa taagttgtga gatagagtgt ttacaagtgt atatagataa taaatttgag
     6961 gtttaagatt aactaagtgg gtttcaggta catcgcgaaa ataattataa gttggagatt
     7021 ttgtatgttt aagattaact aagtggattt caaatattga tatagtagat ataatagaag
     7081 ttttattatt aggtttaaaa ttaactaatc ggatttttaa atattctgac tattatataa
     7141 ggatattatt agcttgaatt taaaaccaat aatataaaac aacaaaatat tatatttatg
     7201 atataataaa agttatggaa aaacaggaat attactatag agaagagagg taaacttatg
     7261 aataattgga tagaagtaac tataaaaaca acaacagaag cagttgaacc tataacgaat
     7321 atactatatg aacaaggagc tggaggagct gtaatagaag acccaaaaga ctttttattc
     7381 caaaagaaaa atgaactaga ctgggattat gttgaagaag aagtatttaa aaagaatgaa
     7441 gaagatgatg ttcttataaa aacttatgtt tctgaagaaa aaaatgtgat ggaatttgtc
     7501 gaaataataa agcaaaaagt tttagggtta aaagattttg gtatagatat aggtgaagga
     7561 agtgtatcat tggaccaagt aaatgaagct gattgggcaa atgcatggaa agcatactac
     7621 aaaccaacaa aagtaggaca aagggtagta gtaaagccta catgggaaga ttatgctatg
     7681 caagaggggg atttaatcat agagcttgac cctggaatgg catttggtac aggtacacat
     7741 gagactacta gtatgtgcat tagagaatta gaaaagtacg tgaataaaga ttctaaagta
     7801 tttgatattg gctgtggtag tggaatcctt gcaatagcag cagctaaatt aggagcaaaa
     7861 gaagttgtag ctgtagattt agatgaagtg gctgtaaaag ttgcaaaaga aaatgttctt
     7921 gaaaataaag ttgaaaaaag tgtatctgta atgcatggta atttaactga tgtaataaaa
     7981 gataaagcgg atgtaatagt tgcaaatata atagctgata taataaagat acttgcaaaa
     8041 gatgttcaaa actttatgaa agaagatgct atattcatat cttcaggtat aattttggat
     8101 aaagtagaag aagttaaaga aagcttaatt gaaaatggat ttgaaatagt tgaagttcaa
     8161 aaattaggtg aatggtcagc aatagtttct aagcttaaaa aataagggat atggtgacgt
     8221 ataatggata gattttttgt tgaaaaaaat aatataaatc tacaagataa aacttgcact
     8281 atagaaggtg aagatgtaaa acatatttca aaagttttaa gatgcaaatt aggtgagaaa
     8341 cttgaaatat gtgacaagaa taataatgag tatatttgtg agataatgaa tatagataag
     8401 agtatagtta atttagaaat tttagaaaaa gttgatataa atagagaatc agaacttaaa
     8461 gtaaggctat accagggact tccaaaagct ccaaaaatgg aaatgatact tcaaaaatta
     8521 actgaagtgg gtgtagaaga aataatatta gttcaaacta agagaagtgt tgttaaagtt
     8581 gatgacaaaa aagaagataa aaaatttgaa cgttgggaaa gaataattta tgaagcagca
     8641 aagcaaagca aaagaggtaa gataccaaaa ttaagaggtg ttttaagttt taaagaagct
     8701 ttagaagata tgaaaaaaaa taatgtaaat atttgccctt atgaaaatga aagaacagtt
     8761 tcaataaaac atgcactaaa aaaatgtgat tctaatatag atagtgttgg aatttttata
     8821 ggtccagaag gtggattttc tgaagaagaa atagaacaaa tacaaaaaaa taattgtaat
     8881 gttgtatcat tggggcctag aatattaaga actgagacag cttctgttgt agcatcaaca
     8941 atagctttgt atgaactaag tgatttagga ggagaaaaat aaattgaaaa aagtagcttt
     9001 ttatacactt gggtgtaaag ttaatcaata tgaaacagaa gctatgctag aactatttga
     9061 gaaagatggt tatgaacaag tcaatagtga agagtatgct gatgtttatg ttataaatac
     9121 atgtacagtt actcatatga gtgatagaaa atctcgtcaa tatataagac gagttaaaaa
     9181 gaagaatcca gatgcaataa tagccgttgt aggatgttat tctcaggtat ctccagaaga
     9241 aattttggat atagaagaag taaatttagt catgggcaca aatgatagaa gaaagattgt
     9301 tgaagaaatt aaaaagataa attcaagtaa gaaagttagt acagttgatg acataatgaa
     9361 agttaaagct tttgaagaaa tagaaatcag ccaaactaat ggaaagacta gagcatttat
     9421 gaaaatacaa gatggatgtg acagattttg tacttattgt atcattcctt atgcccgagg
     9481 acgcgttaga agtagagata ttgatagtat agtcgatgaa gtgaaaaaat tggctaataa
     9541 tggctataaa gaggttgtat taacaggaat acatgttgcc tcttatggaa aagatttaaa
     9601 ggatagggat attaaacttc ttgatgtaat aaaacaaata aaccagatag aaaaaataga
     9661 aagaattagg cttagttctg ttgaaccaat tttatttaca gatgaatttg taaatgaagt
     9721 tttaaagatg gataaagttt gtcctcatta ccatttatca cttcaaagtg gatgtgatga
     9781 aactctaaaa agaatgaata gacgatacac aactttagag tacaaaacta ttgtagatag
     9841 attgagaagt aaaatgccag atgtggcaat aactacagat gtaatagtag gttttcctgg
     9901 agagacaaat gaagagttta aaaaaacgta cgagttttta aaagaaatag agctttcaca
     9961 aatgcatatt tttaaatatt caccaagaaa aggaactcca gctgcaacta tggaaaatca
    10021 agttgaccct caaatgaaac actttagaag tgaacaactt ttaaatttaa gtaaagtaaa
    10081 ttttaataaa tttgcaacta aatttatcgg ccgtgagttg gatgttttat ttgaacaaaa
    10141 tatagaaggc aataaatatg agggattaac ttcaaattat ataagagttg ttgttgaaag
    10201 tgacaaaaat atacaaggtc agatattaaa agtgaaaata aatgatgtaa aagatgaata
    10261 tgtagaagga attttgctat aacatgtaga aattataata tttataagat aggaggtgga
    10321 aagaatggat tgtatttttt gtaaaatagc gaacggagaa ataccttcaa ctaaagttta
    10381 tgaagatgat agagtcttag cttttaatga cttgaaccca gttgctccat atcatatatt
    10441 agttgtacct aaaaaacatt atgatagttt aattgatatt cctgataaag agatggacat
    10501 tgtgtcacat attcatgttg ttattaataa aatagctaaa gagaaaggtt ttgaccaaac
    10561 tggattcaga gtcataaata attgtggaag tgatggaggg caagaagtta aacatttgca
    10621 ctatcatatt ttagctggta aaaagcttcc taattacgaa gcgggacaaa actaaactaa
    10681 attattgaaa aattgagttc cttcttgata aaaatacttg ataaaagtaa aaatttagtt
    10741 tataataaac aaagtgttag gttgtaatgc atattatgca aaaagaacat agttacagtc
    10801 ccagcataat tgcgctattg gagggaggga aaaagaaatg tcagaagtaa gagttagaga
    10861 gaatgaaaca ttagacagcg ctttaagaag attcaagcgt caatgtgcaa tgtctggcat
    10921 catgtcagaa gtgagaaaaa gagagcacta tgataagcca agcgttaagc gtaagaaaaa
    10981 agctgaagca gcaagaagaa aaaacgctaa gaaatagtag taaataagaa taaagaggtg
    11041 aaggggatgt cccttaagca aaagttacaa gaggatttga aatcttcgat gaaaaacaaa
    11101 gatacagtga gaaaatctgt agtaacttta ataagagctt ctataaagca gtatgaggtt
    11161 gataataggg tcgagcttga tgaagatgga attatcgatg taatagcaaa acaattaaag
    11221 caacgtagag atgcattagt tgaatttgaa aaagctggaa gagaagactt aataaaagaa
    11281 acagaaggtg aaattgaagt tttaaaagag tacctacctc aacaattaag cgaagaagag
    11341 ttagaagaaa ttgtaaaatc tactatatct gaagtagggg caacttctat gaaggatatg
    11401 ggaaaaataa tgtcagttat ccaaccaaaa gttaaaggaa gagctgacgg aaaacttata
    11461 aataagttgg taaaacaaaa cttacaatag attgatatag cctagagaga aatctctagg
    11521 ctttttatag tttttgggaa taacgagtca tatttgagca tatctataat caaagaaaat
    11581 tttgaaagaa ggggaaaaat ttgattatat cagtaaaaga gattaggaat tttttgataa
    11641 acatatttat accactaata ataggttact taagtagcat cttaacaatg attatcagtg
    11701 gaacagatat ttcaacctat tatttacaac tcacaaagcc aagctttgca ccaccagctt
    11761 ttatctttcc tatagtttgg actatattgt acatattgat gggtatatct tcatacctaa
    11821 tattaagaaa gggttacaac ttacctaaag taagagatgc aattttttat tatggattac
    11881 aacttgtatt aaactttatt tggagtatac tattttttgg atttggactt agatttacag
    11941 ctttgataga tataataata atgattttgg tttctataat aatgatttcg aagttttcta
    12001 aaatagataa gagatctgga tacataaatt tgatttattt aatgtggctt gtttatgcag
    12061 gatttttgaa ctattttata tggtttatta ataaatagaa tgtaaagatt ttgtaaatta
    12121 ccctgtactt ttaataatat cataacaaac gaaaaataaa tagactatta aaaataatca
    12181 ttttcatata caataatatt aatttaaata caataaattt ataaaaattg tatttaaaga
    12241 ttaacatatt aaagtattag aattggaaag gcttgaaggg agaggttttt atgcaaataa
    12301 tagtatctgg gagacaaatg aaattaactg acggaataaa aggatacgta gatggaaaat
    12361 taagtaggtt agaaaaatac cttgatcctg aatcagaagt gaaagtaaca gtaagtgcga
    12421 aaaaggatag acaaaaagtg gaggttacaa taattcctat taacggtcaa ataataagag
    12481 cagaagacgt agaagatgac ttatatgcag caattgatat tgtttgtgac aaattaagta
    12541 gacaagttgt caaatataaa actaaagtta aagacaaagt tcaaaataat aagagcataa
    12601 gatttgaaaa tcttgacttt atagataata gcagtgaatt tgatgattat gattatgatg
    12661 atgaagaaga tgagaatata gtaatagaaa gaagaaagaa atttaatgta aaacctatga
    12721 gttctgagga ggcgatactt caaatggaac tagtaggaca caatttctat atgtttagaa
    12781 accaagataa ttttgaaata aacatagtgt ataagcgtaa agctggtgga tatggtttaa
    12841 tagaacaaga ttaataaaaa ttgtatacgt ttagatttta tataagaaaa aataaaaagg
    12901 taatcatgtt gattttttat caatatgatt acctttttct tgtttaaata atatattaga
    12961 tattaggtat ttattatata aaaattttaa aactaatctg ttacaacatc aataaagtgc
    13021 ataaatacct atttcaagca taaaattaat taagggggta atgcttatgt tagaaatatc
    13081 cactgattta caagtaaatc aaccagttat tactgttaca agtaatacgt ttattagtat
    13141 agaaaattat ctatcaatat tagaatatga agttgattta ataagaataa agactaaagt
    13201 taaaacaatt aaaatatctg gggataaact ttcacttaag tatattactg atagtgagat
    13261 aggtataaaa gggattatct acaatgttga gtatgttgat taggaggaga tacacttgat
    13321 aagttttata aggggatact atgttatagt tgtggaagga gtaggactcg aacaattttt
    13381 aaatcatctt ataagaaatg ggataagtgt ttacaatgta actagaataa aaaatactaa
    13441 aatggagttt catatagata gacaagatat aaaagaattt aagaatgttt atagaggaag
    13501 taagtttgat ataaaggtaa agcaaaaaac tggggtccct tttattatca aaagagttta
    13561 taagcacaaa ggtatgtgga tttgtgctct tgtttcttta tttttattga tgtctacatc
    13621 acagtttgtg acagatgtat atatacaatc tcctgaaggt ataaaaaaag aagctcttag
    13681 aaatgagctt tataaggttg gagtaagacc aggagtctat aagaagagta ttgatagaaa
    13741 agaagttaga gaccatatga tgtcaaaatt taatgatgtg gcatatttat ctatcaatgt
    13801 aaaaggaaca aatatatttg ttactgttac aaaaaaggca gaatccctaa aatctacaga
    13861 ccaatcaaat tattgtaatg taattgcttt aaaaaatggt ataatagaaa aggtaatccc
    13921 tagaagtggt aaatcagtta ttaaatctgg agacatagtc caaaagggag atgtcttatt
    13981 aaatggagct aataccaagt caataccaga agtatgggca agtacttttt atgagtccac
    14041 aaaaaaggca agttatgtag atactgtaaa taaaaaaact ggagaaaaga aaaatatata
    14101 tacactaagt ttttatgata aagagtttac aatgagaaaa aatataaaat ataaagatta
    14161 tgttgttgaa aataaagaaa aaaaactatc aataggaaac tatacttttc caataaagat
    14221 aaaaacgagt actttttatg aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt
    14281 aaaaaaagaa ttatctgaaa aagctttaaa agagttagaa tacataatcc cagcttcggc
    14341 tagaataata gatgtaaaac ataactttaa ggttaataaa aatatgttag aatatttaat
    14401 aacagttcaa actagtgaaa atattgctaa aatttatcct cttagtaagt cagaagcaga
    14461 aaggtttata aaggaagaaa gtaaaccaga tgaaggtgaa gaggaagttc cttccaatcc
    14521 agaaaaaaga ccattaaatg atataagaaa tgagtttgat gaggataata aggataataa
    14581 agatcaaaac aattctgatg aaaataatag taatcaaaat agtaataata gtcaaaataa
    14641 taatagcaat aacaactaat aaaattaaac ttaaaatctt aggaggacta attcaattga
    14701 taatacaaaa gaaatttaca atagcagatg gtaactttga aagagagtta tttggcaact
    14761 ttgatgaaaa tgtaaaatta atagagaaga ctttaaatat agatgtaata cttagagaag
    14821 gcaatataat tttaattgga gaagaaaaaa atgtagattc tgcattaaaa cttatgaatg
    14881 aattacacca aactgtatca aatggtaagc atttagataa gcagagtatt tcttattcat
    14941 tatctctttt gcttgaagga agtgaacaaa aaataaaaga acttgagggg acaatcgtca
    15001 taactcaaag gggaaaagct gttcaaccta aaactttagg tcaaaaagaa tatataaaac
    15061 ttatagaaaa taatgatata acttttggtg ttggacctgc tggaacagga aagacttatc
    15121 ttgcggttgc tatggcagta aaagctttta aaagagatga agttagtaga attatactta
    15181 caagaccagc tgtggaagca ggagagagtc taggtttttt accaggagat ttaaaggaca
    15241 aagttgaccc atatttaaga ccactatatg atgccttatt tgaaatgttg ggtgctgata
    15301 aatttaataa gtatttagaa agaggtacta ttgaggttgc accacttgca tttatgagag
    15361 gtagaacact tgataattca tttataatat tggatgaagc tcaaaatact acttctgaac
    15421 aaatgaaaat gtttttaact agacttggat ttggctctaa agctgtagtt acaggagatg
    15481 taactcaaac agatttacca caaaataaga agagtggtct aatacaagct acagagattc
    15541 ttaaaggtgt tccaggtatt gggtctataa tgctaacaga tagagatgtt gtaagacatg
    15601 agttggtaca aagaataata agagcttatg agaagcacga caaaagagaa gaatttaaaa
    15661 aagaagaacg taaaaaagcg aaaatgcagg aaaagacttt caaaagaaag taaaagtgta
    15721 gtaatataga tatatagcat tattaatgaa taaaaagtag gtgtagtgaa tggatttgat
    15781 actagatgat agacaagata aattagaagt aagtgaggag ttaattgaaa agataaagga
    15841 tataatcata gaatgtttag attatgaagg atatgatgat aattatgaag tgagtctttc
    15901 ttttgtagat aataaagaga ttcatgaatt aaatagagaa tatagaggag ttgatagagt
    15961 gactgatgtt ttatcatttc ctcttttaag tgatgatttt gaggatgttg agttagaaga
    16021 agaatcccta ggtgacatag tagtatctct tgaaagagcc ttagaacaga gtatagagta
    16081 taaccatagc tttgaaagag aagtatgttt tttaatatgt catagtatgt ttcatctttt
    16141 aggttatgac cacgatacag atgaaaatac aaaagaaatg agagaaaaag aagaacacat
    16201 attaaataag ttaaacataa caagggagta gtttttatga aaccagaaaa aactcgtcaa
    16261 ggtataatta aagcatttaa tgcagctata gaagggatat tgtatacatt taaatttgaa
    16321 agaaatatga aaatacatta tttaggttca gttgctgttc ttataataag tttgtttttt
    16381 aacttttcaa aattagaaat gataatgtta cttatgtcca tatgcttagt tgtagttgct
    16441 gaaatgttta atacagctat agaaaaagct gttgatttag taactgatga atatcatgtt
    16501 cttgctaaga tagcaaaaga tgttgcagct ggaggcgttc tagtggcagc actaaattca
    16561 gtagtagttg gatatatttt gttttatgat aaattgacag atatatctgg aatattaatt
    16621 tataaaataa gagaatcaga attacatata accttgatat gtatattatt ggttcttata
    16681 gctgttgtag ttgtaaaagc attgacttct acaggtactc ctctaaaagg aggaatgcca
    16741 agtggtcatg ctgccttagc atttgctata gcaactgcaa ttacacttat gactgaaaga
    16801 gttgttgcct ctactcttgc atatataatg gctgtattag ttgcccaaag tagaattgaa
    16861 ggaaaaatac ataccttttg ggagactatt gctggagctt tgttaggtgt gttgatagct
    16921 atattagtat ttcagcttgg aatgttctat aattaaaata aatgtacata aaatatttaa
    16981 aataaaacaa atctgtgcta attaaatttt tatgatataa tgtagagtat taaataaaaa
    17041 taacttttaa ttattgtata caggaggagt tgttgtggat aacatagaat tgttaaggtt
    17101 ggcagaagat gccagacaac attcttatgc tccttactca ggttttagag taggtgcagc
    17161 tcttttaact aagagtggta aagtttatac tggatgtaat atagaatgtg catcattagg
    17221 tggaacaaat tgtgcagaaa gaacagctat ttttaaagca atatcagagg gtgataagga
    17281 tatctataaa atagctatag caagtgataa ctcagaaaat aatgagcaaa catatccatg
    17341 tggaatatgc aggcaggtaa ttatagagtt tggttctgat ataaaaataa taacgggata
    17401 tactaaagga gaggtgtttg agcatagtat aaaggatttg ttgccaaact atttctctgg
    17461 aaatgattta aaataaattt aaaagtataa ataaatttaa gtaattaaaa caagaagata
    17521 aaaagttagg agacagttaa tgttcaaatc aggttttgtc agtatagtgg gtagaccaaa
    17581 tgtaggaaaa tctactttaa tgaataatgt agttggagaa aaaatagcta taatgagtga
    17641 taagcctcaa actacaagaa atacaataca agcagtatat acagatgaag aaatgcagat
    17701 agtattctta gatacaccag gtattcacaa accaaaaaat aaattaggtg aatttatggt
    17761 aaaggcagca acagaagctt ttaaaaatgt agatttaata ttatttgtag ttgatgattc
    17821 taaaaaaata ggtcctggag atagaaagat aatagaggat ttaagaagtg ttaagacacc
    17881 aataatttta gttgtaaaca aaatagacca actagaccaa aaagatgagc tatttgatat
    17941 aatcaaaatg tatgatagag aaggcatttt taaagaaata gtaccaatat cagcattaaa
    18001 aggaaagaat acggatacct taataaaagt tatacaaaat tatttagaag aagggccaaa
    18061 gtattttcca gattatatga ttacagacca gccagaaaga gtattaatag cagaacttat
    18121 aagagaaaaa gttcttcact atcttaatga tgaaatacct catggtgtag ctgttgaaat
    18181 tgaaaaaatg aaagcgagaa atgataagga aatagtagat gtatctgctg taatatactg
    18241 tgaaagagat tcacataagg gaataataat aggaaaaaat ggtaggaaac taaaaggaat
    18301 aggtaagtca gcaagacaag atatagaatt gcttttaggt tcacaaataa atcttcaatt
    18361 atgggttaaa gtcaaagaaa attggagaaa tttacaaaat tatataaata attttggata
    18421 taatgataaa taaaggtggg ttatatggat aggaaactgg atgcttccca agatttatta
    18481 tatgaagtaa aatcattaat tgataataat aaagtattgg agttaagaga attaatagag
    18541 gaatatcata taattgatat attcgatata atggaaaatt tagaggaaga catgaaaatt
    18601 caattatttg aagtacttcc tttagatatg gcatcttcta tattagaaga aggtagtgta
    18661 gagtttttta ttagtatttt atctaaactg gatgtagaac actcgaaaaa tatacttgaa
    18721 cttatgtctc ttggagatat ggcagacaag ttgagtgaac ttgaagaaga agaaagagaa
    18781 cacataataa atcttttgaa tcaagagaat gccgattatg taaaagagtt actattctat
    18841 gatgaagatt ctgctggtgg aacaatgacc actggatata tttctataaa taaagatatg
    18901 acagctcttg aggctataga ccatatgaga gaagaagctg aagaagctga aactatttat
    18961 tatatatatg tggttgatga tgaagaaaaa ctagttggag tattgtcatt aagagaatta
    19021 ataatagcta gagacgcaaa tatagtagaa gatttgatga gtgaaaatat aatctctgtt
    19081 tatgtagatg aagatagaga agaagcagta agattagtat ctaaatacaa tttaatagca
    19141 attccagtag tagacagaca agaaaaatta aaaggaataa taacagtaga tgatataatt
    19201 gatgtaatgg aagaagaagc aactgaagat atgtataaat ttgcaggaag ttcagaacat
    19261 gaaagagaag ttgctgaaaa agaaaaccct acattgagag agcaaataat atcagcactt
    19321 agaggacgat taccttggtt aattattacc ttagttggag ggttgttggc ttcgttaata
    19381 ctttctaact tagactatat aatgaatcct gtatatgctc cattagtatt ttttatacct
    19441 gtagtaattg gtatgggtgg aaatattgga actcaatcat catctgttac agtaataaca
    19501 ttatctaata aggatttaaa ttttagcaat gtggttagag aaggtattgt aggtattatt
    19561 acaggtcttt tgtgtagtat aataacaggt attgtaattt actttgttat gagaaagtta
    19621 gacattgtat taattgtttc aatatcatta tttataaata tggttttagg tgcaacaata
    19681 ggtgcgttta tgcctgtttt attaaaaaaa atggatgcag acccatctac agtttcatca
    19741 ccaattatat ctacagctct tgatataact ggtatagcag tttactttat aataacaacg
    19801 gcattattgt caaaaattgt ttaataactt tatgtatgat ttatcctctt cttactaaaa
    19861 atataagtat aaatgtaaga agggggtttt ttaaaaatga acaatatcag ttgggaagtt
    19921 tttaaaaaaa caggtagtat agatgcatat ttatatttct gtgattgcaa aaacatacat
    19981 gaagaaattg aagaagtaag ggagaaaaaa gaagacgatg ataatcctga acacccaggg
    20041 gatagtactt aaagctataa ggtataagga aagtgatata atacttacac tatttacaag
    20101 aaagcttgga aaagtatctg caatagcaaa aggagctaaa aaaaataaga gctctttgct
    20161 ttcatcatca cagttatttt cttatagcaa ttttacctta aaaaaacaag gtaatatgta
    20221 caaggttact caaagtgaaa taataaaaag tttttataat atatcctatg atatagaggc
    20281 attttcttat gctacatata ttacaaaatt agttgaaaat tctatacttg aaaatcagac
    20341 taataatagg ctatttatat tacttgcgca gactctgtat ttgtatacac aggataatac
    20401 agacaatagg tttataacag cagcattcga gttaaaattt ttagactata taggatttaa
    20461 acctatagta aataaatgca ttaattgtga atgtaaaatt ttacaaaatt ctgtgtttaa
    20521 tatctacgaa ggtggtatac tatgtagtaa gtgtagtaaa ctttttgata ataatataaa
    20581 attagactta acaactataa gtcttatgga atatgtatta agaaatgata ttttaacatg
    20641 tagtaaagct aaagtatcaa aatatataac acatgaactt gagaatattc tagacaagta
    20701 tttgaaagtt tatgttgata atattaattt taaatcatta cacgtattgc aaagtgtaaa
    20761 aaataataag ggagtggata atgatgagta gtaatataac tattgaaatg gttgattcag
    20821 tctttgaaag agttccagga gcaacttatg cagaagcaaa agaagcatta gtaatgtgca
    20881 atggagatgt aattgaggca gtaatatatt tagaaaataa aaaaaatact tgtaattgta
    20941 aagcaaaatc tgctaaagaa actatggaag aagtatttgg caaagatgga gaaaatataa
    21001 agtttcaatt aaaagagctt cttagaaaaa gtagtgtagt gagaataata gttgaaaaag
    21061 atggaaaaac tatgatgaat attcctttaa cagttggagt tgttggatta gcttttgtac
    21121 cattagctac tttagttgga ttatcagctg ctgttataag taaatatcgt ataaaagttc
    21181 aaaatgaaga agatggagaa attgtagatt taggtgaatt gaatgaagaa aaacttcacg
    21241 ttttgaagaa tatgataact aatgcagcaa aagatgtgaa ggatgtagta gttgacaaaa
    21301 aagaagatga caaagatgta actgctgacc ttatgaaaga agaatctgaa aagaataaag
    21361 aagacgaaga cgaaaataaa tagatataaa gtgactttta aaaaatgaag agaagataat
    21421 tataagaatt atcactaaca ttgacaaatt ttatagaatt tgatattcta aaagttgtag
    21481 aagaactatt aaaatagcct ttcgtgtaga cgagaggctt tattgttata ggagggattt
    21541 ttatgaattt tcaagagatg atactggcgt tgcaaaaata ctggtcaaag caaggttgta
    21601 tcatgatgca accttatgat attgaaaaag gtgcgggaac tatgaatcct aatacttttt
    21661 taagatcatt aggacctgag ccttggcaag tatgctatgt agagccttca agaagaccag
    21721 cagatggaag atatggtgaa aacccaaaca gactatatca acatcatcaa ttccaagtta
    21781 tactaaaacc ttctccagat aatatacaag aattatattt ggaaagtttg aaagagattg
    21841 gaattgaccc aagtgagcat gacataagat ttgttgaaga taactgggaa gctgctacag
    21901 ttggtgcttg gggacttgga tgggaagttt ggctagatgg tatggaaata actcaattta
    21961 cgtattttca acaagtaggt aatattgaat gtgagcttga gacaggagaa attacatatg
    22021 gattagaaag attagctatg tacattcaag aagttgatag tgtttatgat ttaaaatgga
    22081 atgataaaat aacttatggt gaagtattta ataaagctga gtatgaaaac tcaatgtacg
    22141 catttgaatt atgtgatgca gatatgttgt ttaatctgtt tgatatatat gaaaaagaag
    22201 cattaagact tatggaaaat ggccttgtaa taccgtcata tgactatgtg ttaaaatgct
    22261 ctcatgcatt taatacattg gatgcaagag gtgcaatagg agttagtcaa agagcttcat
    22321 ttataggtag agttagaaat atggcaaaga ctgttgcaga aacttttgta aaacaaagag
    22381 aagaaatggg attccctctt ttaaaggacg gtgacaaata atgaaaaatt atcttttatt
    22441 tgaaatcggt gttgaagaaa tgccatcaag atttgtaggc agtacactag aacaattaaa
    22501 aaacaatctt agtaaattat ttgatgaaaa tagaatagct tttgataaaa taaatgctta
    22561 tgggacacca agaagactta ctttagtagt cgaaggattg agtgatagac aaagtgattt
    22621 agaagaggaa ataaagggtc ctgctaaaaa aattgctgtt gatgcagaaa ataatttaac
    22681 aaaaccagca ttaggattta taaaaagcaa aggattaagc gaatctgaca tatattttaa
    22741 acaagttgga aaagaagaat atatatttgc gactataaag caagatggaa aagaaacttc
    22801 agaagtacta aaagacatat taccagagac tataaaatct gtagtattcc caaaagctat
    22861 gcgttgggga ggaaaaaatc ttaaatttgc aagacctata agatggatag ttgcattgtt
    22921 aaatgataaa gtattagagt ttgatttaga aggaataata tcttctaatg taacaaaagg
    22981 tcatagattt ttaggtgaaa gtacaataga agttgattct atagaagatt attttgaaaa
    23041 attagaaaaa aattatatag ttttagacca aaacaagaga aaagaaatga taagaaaaca
    23101 atgtatagaa gtagcaaatt ctttaggtgg agaagttgaa tttgatgaag atttacttga
    23161 agaggtaact tatttagttg aatatccaac tgctttttat ggagaatttg atgtggatta
    23221 tataaaacta ccaaaagaag ttgttataac tcctatgaaa caacatcaaa gatattttcc
    23281 agtacttaaa gaaggaaaac tgttacctaa ctttatagct gttagaaatg gtgattctta
    23341 cagaatagac aatgttaaag ctggaaatga aaaagtttta gaagcaagac ttgcagatgc
    23401 tttatttttc tatagagaag atactaagag aaacttagaa agctacatag aaaaattaaa
    23461 aactgttgta ttccaagtaa aattaggaac tatttacgac aaaactttaa gacttgaaaa
    23521 attaagtgct gatatacttg atagtttagg tttagtagat gaaaaggcag atactataag
    23581 ggctgctaaa ttatctaagg cagatttagt tactggaatg gtaggagaat ttgatgaatt
    23641 acaaggtttt atgggaaaag aatacgcaaa agttggagga gaaaatgatg ttgtaagtga
    23701 agctattttt gaacattatc ttccaagatt tgcaggagat atacttccta agactaatgc
    23761 aggggttgcc ttatcaatag ctgataagtt ggattcaata gcaggtttct ttgctatagg
    23821 aatacaacca acaggttctc aagacccata tgctttaaga agacaagctt taggtatact
    23881 tagtatactt atggacagaa aagttgctgt ggatataaaa atacttattg aacatgcttt
    23941 agacaattat tctgaattag attttaataa agaagaagtt gttgagcaaa taatgaattt
    24001 cttcaatgag agagtaaaaa atctatttaa agatttagga ataagatatg atgttgttga
    24061 tgctgtttta agttctgata taaatgatgt ttctgatatg catatgagag ctttagagct
    24121 taataattgg ctagaaaaag atgaattagt ggagatgctt actgcattta atagagtttc
    24181 tactctagct caaaaagcag aaagtgataa aattgatgaa tctttattaa aagaagatgc
    24241 tgaaattaaa ctttacaatg aatttaaaca agtaaaggta aaagttaatg aattattaaa
    24301 ggataagaaa tatggtattg ctttagatga atttgcatct ttaagaccag tagttgataa
    24361 tttatttgat acagttatgg tcatggataa tgatgaagct attaagaaca atagacttgc
    24421 tttacttaaa caagtatatg atactatgct aggaatttgt gatttatcta aaattgttta
    24481 taaataaaat ttaaactatt tataagaaat aaggctgtct caaaatagag atagtcttat
    24541 ttttcattgt tgagatgatg aaaatatatt tgatttcata gaaaaagagt gtctcataaa
    24601 ctaaaaagtt cattttgaga cacccatgtt ttttgtaagt aaatataata aattatattt
    24661 tatacttatc agttattttg tttaaaatat tatcatttga atttacccat tcataaaaat
    24721 tgcctgaaga tgcgtccgat attttataaa gcatttcagg aatatcacta gctttaaaat
    24781 ctccaagtga ttttcccaat ctagtcttac ctatttcaaa actgcctcca acaaataatt
    24841 caaatgcatc tgtagatata ccatcaacat tcttcttttt accacataaa cctatgctac
    24901 caacttgatg aacaccacat gaatttaaac atcctgatat atataactta ggtgctttat
    24961 ttaaaatttc ttcattattt tgtaatctaa aataatctat gatttcatgt aacattttct
    25021 gactattttg tatgcccatt tgacaaattg gaacacctat gcaagatata ctttgttcta
    25081 attgagaatt acaactaata gttttactta tttctaatat tctttttgct tcatttccat
    25141 ttaaatttaa aatatataat ccctctgtca ttcctagtct aatcatagga ttttcaacat
    25201 tatctaattc tttaagtaaa gtacttaaat cctttgttaa caaaatacct ccaactggat
    25261 gtatatatac agtgtataaa ccatcctgtt tttgaggtat tagtctagaa tcttgtatat
    25321 ctattttaac tccaggttta gagtaatcta tttcttcaat attgagttca agactaccat
    25381 tttctttttc taatttatag tattctttaa atttttctaa gaatagttct tcaccaagtg
    25441 attcgaccat atatctaacc ctagctctgt ttttattttc ataatttcca taatccataa
    25501 agaatttaat cataccttca acacaatata aggcgtcttt cggctttata agttcatcaa
    25561 gttctaaagc aactttgggt tgtttaccta gacctccacc taagaaaact ctgaaatatg
    25621 gttcatcatt tttcaaagta gcaacaaatc ctaagtcttg tatactacaa tgagcagtat
    25681 ctgtgtaaca attagaataa gaaactttta attttctagg taaatggtat gtagtagcat
    25741 ttttgataaa atatttatca gtagcaactg catatggagt aacatcaaag acttcactag
    25801 ggtcaactcc agataatgga gataatccga catttctagg ataattacca ccaccacctc
    25861 tagtgaatat atcatttttt attccttctt ccatgatatc aacaattgaa tttatagata
    25921 aatcgtgtaa ttgtatagct tgtctagttg ttaagtgtat cttatctaag ttgtgtttaa
    25981 tagctatttc atagatttta ttaagttgat actgagataa aactccagca gatactctaa
    26041 gtcttatcat aaaagatttt ttgtctcttt gagcataaac accatatcca ccagaaaatc
    26101 ccttatattg cattttattt atttctccat ttaaaaattt tgaagaatat tctttaaaat
    26161 taggtagttc atttaacaat atctttttaa aattatccat tatttcacct cattgttagt
    26221 cttgtataaa aatatatcta ttgtataata gatatatata ttattatcta gtcaggataa
    26281 aaatataaca gaataattct attaaaagta agaataataa ggacatagtg agtattttgt
    26341 ttttatttta caaattaagt ataatatttt tgttttataa aatataatta agataaatag
    26401 ataattttgt aattttaata tataatattt agtagagtgt gggagtagca cttaatattt
    26461 gcaggaggag tttttgtata tgggagaaat aataaatgca ttagatagag ccctagacat
    26521 aattttgctg ttatatcatg aaaagagaga aatgggaata actgaaataa gtaaggctat
    26581 gggagtatat aaaagtactg tacatagaac tttggttact cttgaaaaca aaggttttgt
    26641 tatacaaaat gcagagaatg gtaagtattg gctaggaata aatttatatg caataggtat
    26701 ggttgtggga gaaaaaatgt cattgactga aatagtaaaa ccatatacga aaaaattaaa
    26761 tcaagaattt aacgaagtag ttaatgtatc tatattagaa gaaagagctc aagattctcc
    26821 aagaagtatc ataatacata aagagtatgg ttcaaatcaa ttactttcag taaatccatc
    26881 cgttggttca agtagtgaat gttattgttc agcagttggt aaatgtctta tggcatttaa
    26941 tgattctata gattttgaaa aatatagaaa aacaccaata cacaagtata cagaacatac
    27001 aattgataat tgggatgata tgatgctttt tttagcaaaa ataaaagaac aaggatatgc
    27061 tatagatgat gaagaactag aacatggact aacttgtatt ggagcaccta tcttagataa
    27121 aaataataaa gctattgcag ccataagttt gtcaggtcca actataagaa tgagagaagg
    27181 ggactttgag tataagatta aaagagttat agaaactgca aaaagtatat ctgaactttt
    27241 tagatgattt ttaatctcga taataaaaat aggctatatt taaattgtta tttgtaaata
    27301 tcagtttaaa tgtagcctat ttttctttaa tctaaaatta aatattctta aaatttaaaa
    27361 tgttattgaa atatataagc gaatgtgtta tcttttaatt aagcaataaa aatagaacaa
    27421 ggttctatac tatagaacaa aattgatgca tatatagtat ttatacaaaa tataaatatt
    27481 atatatttaa aattaagtaa aaatattaca taaaggggag atttgagaaa tggaaaaagt
    27541 caagttgagt gttgaaagat gtaagggatg ctatctttgt attgaagctt gcaaaaaaga
    27601 tgcaataagt ctttctgact acacaaataa aaaaggcttt gtaactatta aagtcgatga
    27661 agaaaaatgt attcaatgtg gcgcttgtta tacaatgtgc cctgatttag tatttgagat
    27721 attataaatt atttttatta gaggggagaa aaggtatgca aagtttatta attaaaggaa
    27781 atgaagcttt agcagaaggt gctatacgtg gtggttgcag attttatgca ggttatccaa
    27841 taacgccaca atcagaaata ttagaaatga tggcatggag acaaaaagaa gttggaggag
    27901 tatttatcca aggtgaatca gaaatagcca gtgtaaatat ggttatggga gcaagtgcac
    27961 ttggagcaag agctatgact tcttcatctg gtccaggatt ttcattaaaa caagaaggta
    28021 ttgcatattg ggtatcagct ggtttgccag cagttgtagc atgtgtgcaa agatatggta
    28081 taggtgatgg atttatagga gctggtcaag ataattattg gcaagcagta aaaggcggtg
    28141 gaaatggaga ttatcattta atcgtgttag cacctaatag tgttcaagaa aatatggata
    28201 tggcatatga atcttttgaa ttggctgaaa aatatatgca tccagtactt atactttctg
    28261 atgctacaat aggtcaaatg gtagaacctt gtatagcacc accaatgaaa gaatacgata
    28321 tggataaagc tccatgggct gcaaaaggaa cacctgtagg agaaaaaaat aaagtaataa
    28381 ctgatataac atattttgaa gaccaagata catggcaaaa agggtatata gaaaaaatga
    28441 gaaaagttca agaagatgaa caaagatggg aagagataga aatagaagat gctgaagtgg
    28501 tatttgtggc atacggtatt tcatcaagaa tagcagaagg tgcagttaga gatgctagag
    28561 caaaaggaat gaaattaggt ctaataagac caaaaacatt gtggccttac ccaagaaaag
    28621 catttaagaa attaagtgat tcattgaagg gacttgtaac agttgaaatg aacatgatgg
    28681 cacagatgaa ggaagatgta attatagcat gtgataatag atttccagta tattcagtag
    28741 caacagctca atatatggca gaagtaaatc aattaataga ttttgctcaa cagattatag
    28801 atggaaaagc taagcaagag gaggtatttt aattatggct aaaaaagcac cagcaataat
    28861 gatgtgtgaa aatatgttct gtggaggctg tggacactct atagtcgata gaattattgg
    28921 agaagtatta acagaaatgg atatatgtca agatacaatt atatgttcag atataggttg
    28981 taatcatatg tttcaattta gtatgaatgt agatgttgtc gttccacctc atggtaaaat
    29041 gggagcagca atggcagcta tgaaaagagt tagaccagat aaatatatat ttacaatagc
    29101 tggagatggt ggagcttatg caatagggct tggagaaact atgtcagctg ctactagaaa
    29161 tgataatgta atatttcttg ttatgaacaa taatatgttt gcaatgacag gagggcaaat
    29221 ggctccaact actatagaag gtcaatatac agcttctact ccagcgggta gaaattacgc
    29281 agatcatggt tcaacatttg atgttgtaaa agttatggga aatttagata tagcatatct
    29341 agcaagagga acaataacta accctaaaga aataaataca acaaaaagat acatcaaaaa
    29401 agctctagaa aaacaaaaag aaaagaaagg tttttgctta atagaagtgc ttgtaccatg
    29461 tccaacaaac tggggattaa agccagtaga tgctatgaag agaattgaaa atgaacttgt
    29521 tccaaagtat ccacttggag aatttattga ttagggagga aataatatgt tgaatgaaat
    29581 tatatgtgct ggttttggag gacagggagt tcttacagca ggaaaaatta taatgtatgt
    29641 tgcatataag gcagaaaaga aaattacatg gttcccatct tatggaaatg agatgagggg
    29701 aggaagtgca aattgcaatg ttgttgtaag tgatgaaaaa attgcaaatc cttgtgtaaa
    29761 aaatccagat atactattgg gaatgaacag cgtatcagta gataagtatg aaagtttaat
    29821 gaaaaaaaat ggttatatgt ttgtcaatga atcactagta agtaaggata gaaagtacag
    29881 agaagacatt aatgttataa aagtacctgt tactgaaata gcacaaaatt taggcaatga
    29941 aaaagctgct aacatttgca tgttaggtgc tatggtaaag agtttggatt tatttacaaa
    30001 agaagaattt gaaagtggta tgtgtgaata ctttgaaaat caaggcaaag gtaaattcaa
    30061 ttctaaaaat gtagaagcat ttgaagctgg atacgactac gaatgaatat tttaaatatt
    30121 taaaataatt attgaaatac ttatttttat atgttagtat ttaattaata aaatatagaa
    30181 cgtcgttctg aacaatagga cgaagtcttt aggtcggaat acaagaacga cgttctgtat
    30241 agtagaacta gggggtgtta aattgaaagt tttagtaata aatcctggtg gtacatccac
    30301 aaaaatttct gttttccaag atgaaaatga aattttgaaa aaaaatataa cccatacaag
    30361 agaagattta aaaaattttt ctaaagtatt tgatgaatat gattatagaa aacaactgat
    30421 agtagatatt ttaagtagtg agaatcatag tataaattct tttgacgcag tagttggtcg
    30481 tggtggactt atgaaggcta taaaaggtgg tacctatact gtgagtgaag aaatgattga
    30541 ggatatgaga aatgaaataa atggggaaca tgcatcaaat ttaggtgcat tattagctaa
    30601 aactatagct gatgaaatag gagtacaatc atttgtagta gacccagttt ctgtagatga
    30661 atttgatgat gtatcgagga ttacaggaat atcagacata gaaaaagcaa gttggttaca
    30721 tgcattaaac cataaagctg tttcaagaaa aattgcacaa aaactagggg gaaaatacga
    30781 ggattataac tttattgtag ctcatttagg ttcaggaata tcgattgtag cacataaaca
    30841 aggcaaaatg attgatggaa gtggtggtag gtctgatggt ccattttctc cagaaaggag
    30901 tggtggatta cttgcatatc ctcttataga attgtgttac tccggaaaat atactcatga
    30961 tgaaatggtc gcaaaagtaa gttcgattgg gggaatgtac gattatttag gaacaaagga
    31021 tatgatagaa attgaaaaca gaatcaagga gggtgatgaa aaggcaaaac ttataatgga
    31081 tgcatttatt tatcaagtag caaaagaaat ttctatgtat ggagcttcac tttcagggaa
    31141 tgtagataga gttatcttga caggagggat atctcactca gaaatggtag tcaaaggagt
    31201 agcagaaaaa gtttcatatc tagctccaat agagatagta ccaggagaga tggaaatgga
    31261 ggcacttgct ctaggggcac ttagagtact taatggagaa gaaatagcaa aaacttatta
    31321 atatttagga gaaagagtat gttaaaagat tttaataatt tattagaaga ggctaaaaag
    31381 cttgaacctg ttattgtaag tgttgctgtt gcagaagaca aagaagtatt aaaggctgtg
    31441 aaatcagcaa cagatttaaa cattataaag ccaattcttg taggagacaa ggataaaata
    31501 gaaagtatat caagagaaat tggattaaaa ggtcatgaaa taataggttg tcaaaatccc
    31561 gatgaatcag ttaaaaaagc tgtagagata gttaaaaata aggaagctaa agttcttatg
    31621 aagggattag taaatacaag tgtctatatg agagctatat taaataaaga aacgggtctt
    31681 agaacaggaa gattattaag tttgcttgca gtatatgaat tacctcaata tcataaactt
    31741 ctttattgta ctgatagtgg aataaatgta tcgccaaact taaatcaaaa aaaagatata
    31801 atgacaaatg cacttcttac tatgaaaagt ataggcatgg aaaatcctaa aactgcgata
    31861 cttactgcaa atgaaatggt agaccctaag atacaatcta cagtggatgc caacctactt
    31921 gtagagatgg aaaaatcggg agaaatacca aagtgtatag tggaaggtcc aatagcattt
    31981 gatgtagcat ttgatgcaca tgcagcagaa cataaaggta ttaaaagtaa tattgctggt
    32041 gatgttgatt tattagtatt tccaaatata gaagcaggga atatattagg aaagtcatgg
    32101 cttagattta ataaagctaa gtgggcaggt atagttttag gagcaacaaa tccgataatt
    32161 ttaggttcac gttctgacac agctgaaata aaaataaatt caatagcatt agcgtgccta
    32221 gcatcacaat aagaaattat tattaaggag gaaaaagtat tatgaaatat gcaaaaagaa
    32281 tggatatggt aaaagcttca gctataagag attctcaaaa gaaaatagct cagaaggtag
    32341 caagtggtgg aactgttata tcttttgctg cagggcttcc tgacccaaat ttatttccaa
    32401 tagaagagat aagtgaaata actcaaaaag tacttaatga aaggggaaaa tttgccctag
    32461 catatggacc tactaaaggt gaagatgagt tgttagatct attagtaaaa agaatgaaag
    32521 aagaagaaaa tattgataca aaagcagaaa atattataat aactactggg tcacaacaag
    32581 ggatagcatt aagtgctatg attttattag aaaagggaga tgtagtagtt acagaaaatc
    32641 caagttactt aggtgcaata aatgcattta gaccttatga atgtgatttt ttaggagtag
    32701 atacagatga agaaggaatg gtaacagatg atttagacaa attattatct gaaaattcta
    32761 acataaaggt gatatatgta ataccaacat ttcaaaaccc tacaggaaaa acatggtctt
    32821 tacaaagaag aaaagatttt atggaagtcg tgaataaata tgatgtcata gtaatagagg
    32881 ataatcctta tggagaaata agatttacac cagaacctct acctacactt aaatctcttg
    32941 atactaaaga taaagtattg taccttgggt cattttcaaa ggtattatgt ccaggattta
    33001 gagttgcttg gatgtgtggt aatgtagaca tcatagataa ggcagaactt ttaaaacaag
    33061 gtatagattt acagtcaaat caattttcgc aagtacaagt aatagaattt ttaaagaaat
    33121 acaacttaaa tgaacatata gaaaaaataa gagcagagta taagaaaaga tgtctgctaa
    33181 tgttagatgc tatgaagaaa catttcccag aggaagctaa atatacagaa ccagatggag
    33241 gaatgtttat atgggttgaa cttcctgaaa gtataaatgt agatgattta ttagataaag
    33301 ccattgatgc tggagttgct tatgtttctg gagagtcctt ctttgcaaat aatggtccaa
    33361 aaaatactat gagacttaac tatacaacaa tgtcagaaga acaaatcgtt aaaggtgttg
    33421 agatacttgc agaagttata aaaaaagaac tcaattatgc ttgtgaataa atataaaaag
    33481 tgagggttta aatatgggag aaaaagaaaa cggtataaag tttggaaagt taatgtttca
    33541 acttgttatt atagcaatat cttgggaatt agtttatatt ataccattta tccaatatac
    33601 attatatgat ccaattctaa aagcattaga atgtacaaac actcaactag gttttctact
    33661 tacaatttat gggttaggta atatatttgg agctcctata ggaggctggt tagcagatag
    33721 atttgattat agaaagataa ttttaggttc tgtatttctt aatggagtag tatcattttt
    33781 atttgcattt aatatgaact atccatttgc agttgctact tgggtaggtt gtgcaatcac
    33841 atcattagtt atgaactatc cttctatggt aaaaatactt agagttatag gaaaagataa
    33901 tcaaggtaag gtttatggat ttaatgaagc tatggttgga gtttctggag tagtaatggg
    33961 agcaatattc ttatatattt atacatgctt tgcaactcct acattgggta tgagatgggt
    34021 aatgatatct ttaggaatat tatcaatagc aatgtgtcca gtattatggt ttgtaattaa
    34081 agatgtagat attaaagaag aaagagaaga agcaggtgaa aaaatgtctg ctggtgattt
    34141 tataacagta ctaaaatcac caaatacttg gctagtagga ataagtatat tctgtgtata
    34201 ttcattcaca gttacaatgt cttattttac accatatata acatctgtac ttggaggttc
    34261 tgtagcatta tctggtgcat tagcaataat aagacagcat ggtctaaaat tatttggagc
    34321 accatttgga ggatattgtg cagacaaatt aaaatctcct acaaaagtac ttttaccaat
    34381 atatgtattt ggtatagcag taatagtttt attcctagta ttacctgcat caactccaat
    34441 gactatattc atagctttaa catttgtagt aggtatatta ggttacatgg gaaaaggtat
    34501 atattatgca gtacaagatg aagtaaaagt tccagttaaa tattcagcaa ctactatagg
    34561 tatagctgca gctttaggat tttcaccaga tgtattccaa tttgcattaa taggacactg
    34621 gatagatact tatggcaata aaggttacac atatacattt atattccaaa tagcaattct
    34681 agttattgga atactatctt gtttatatat attaaaggtt aaaaagagaa gagcaagtaa
    34741 attagaagct taaatattca atttggtaat taagtatata taaatacaat acttattttg
    34801 atgaatgtga ttgtataaat aacataaaaa atatcaatag attaagtttt ataataatgt
    34861 ttagagaata aagtataaaa atcaaaatgt tatcaagttt tctaggtttt aacataaagt
    34921 tacccctgaa ggtgtaaaat tgagtggcaa ttattatcta ttttcttacc tacataaacc
    34981 tatttaattt aaattctaag agattttaaa aatagatggt agttgtactc aattttaaac
    35041 ctcataataa atacgaaaat ttaaaatcta actagatact at
//
LOCUS       DAMKMI010000025        34917 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMKMI010000025 DAMKMI010000000
VERSION     DAMKMI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34917
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.026"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(114..1517)
                     /locus_tag="PC429_001170"
     CDS             complement(114..1517)
                     /locus_tag="PC429_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3846685.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(1501..2025)
                     /locus_tag="PC429_001171"
     CDS             complement(1501..2025)
                     /locus_tag="PC429_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3846686.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            2305..2820
                     /locus_tag="PC429_001172"
     CDS             2305..2820
                     /locus_tag="PC429_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF3846687.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            2869..3372
                     /locus_tag="PC429_001173"
     CDS             2869..3372
                     /locus_tag="PC429_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3846688.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            3369..4019
                     /locus_tag="PC429_001174"
     CDS             3369..4019
                     /locus_tag="PC429_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846689.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(4154..4933)
                     /locus_tag="PC429_001175"
     CDS             complement(4154..4933)
                     /locus_tag="PC429_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3846690.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(5045..5296)
                     /locus_tag="PC429_001176"
     CDS             complement(5045..5296)
                     /locus_tag="PC429_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF3846691.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            complement(5625..6494)
                     /locus_tag="PC429_001177"
     CDS             complement(5625..6494)
                     /locus_tag="PC429_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3846692.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            complement(6832..7593)
                     /locus_tag="PC429_001178"
     CDS             complement(6832..7593)
                     /locus_tag="PC429_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846693.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            complement(7609..9144)
                     /locus_tag="PC429_001179"
     CDS             complement(7609..9144)
                     /locus_tag="PC429_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF3846694.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            complement(9558..11798)
                     /locus_tag="PC429_001180"
     CDS             complement(9558..11798)
                     /locus_tag="PC429_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3846695.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            complement(12325..13125)
                     /locus_tag="PC429_001181"
     CDS             complement(12325..13125)
                     /locus_tag="PC429_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF3846696.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            complement(13516..14391)
                     /gene="hslO"
                     /locus_tag="PC429_001182"
     CDS             complement(13516..14391)
                     /gene="hslO"
                     /locus_tag="PC429_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF3846697.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            complement(14676..15422)
                     /locus_tag="PC429_001183"
     CDS             complement(14676..15422)
                     /locus_tag="PC429_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3846698.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            complement(15409..15654)
                     /locus_tag="PC429_001184"
     CDS             complement(15409..15654)
                     /locus_tag="PC429_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF3846699.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            complement(15785..16903)
                     /locus_tag="PC429_001185"
     CDS             complement(15785..16903)
                     /locus_tag="PC429_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF3846700.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            complement(17109..18302)
                     /locus_tag="PC429_001186"
     CDS             complement(17109..18302)
                     /locus_tag="PC429_001186"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF3846701.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            18625..19506
                     /locus_tag="PC429_001187"
     CDS             18625..19506
                     /locus_tag="PC429_001187"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF3846702.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGIISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            19931..20932
                     /locus_tag="PC429_001188"
     CDS             19931..20932
                     /locus_tag="PC429_001188"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF3846703.1"
                     /translation="MKKVNVAIIGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            21049..21936
                     /locus_tag="PC429_001189"
     CDS             21049..21936
                     /locus_tag="PC429_001189"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF3846704.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            22003..22752
                     /locus_tag="PC429_001190"
     CDS             22003..22752
                     /locus_tag="PC429_001190"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF3846705.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            22976..23692
                     /gene="dapD"
                     /locus_tag="PC429_001191"
     CDS             22976..23692
                     /gene="dapD"
                     /locus_tag="PC429_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF3846706.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(24144..25133)
                     /locus_tag="PC429_001192"
     CDS             complement(24144..25133)
                     /locus_tag="PC429_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF3846707.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            25573..26328
                     /locus_tag="PC429_001193"
     CDS             25573..26328
                     /locus_tag="PC429_001193"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF3846708.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHDTLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            complement(26682..28358)
                     /gene="bclA2"
                     /locus_tag="PC429_001194"
     CDS             complement(26682..28358)
                     /gene="bclA2"
                     /locus_tag="PC429_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF3846709.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGVTGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(28736..29275)
                     /gene="hpt"
                     /locus_tag="PC429_001195"
     CDS             complement(28736..29275)
                     /gene="hpt"
                     /locus_tag="PC429_001195"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF3846710.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(29603..30910)
                     /locus_tag="PC429_001196"
     CDS             complement(29603..30910)
                     /locus_tag="PC429_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF3846711.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(31335..33110)
                     /locus_tag="PC429_001197"
     CDS             complement(31335..33110)
                     /locus_tag="PC429_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3846712.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(33107..33244)
                     /locus_tag="PC429_001198"
     CDS             complement(33107..33244)
                     /locus_tag="PC429_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846713.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(33322..33891)
                     /locus_tag="PC429_001199"
     CDS             complement(33322..33891)
                     /locus_tag="PC429_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3846714.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(34075..34710)
                     /locus_tag="PC429_001200"
     CDS             complement(34075..34710)
                     /locus_tag="PC429_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3846715.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
BASE COUNT        11457 a         6061 c         4201 g        13198 t
ORIGIN      
        1 ttctatactt tctaaattag tttattatac ttctttagat taattttcta tacttcctaa
       61 attagtttat tatacttctt taaattaatt ttctatactt cttaaatatt gtcttatacc
      121 tctgcaatct tattatggtt actcatcaaa ttattttgct ttatagtcaa tgatttcatt
      181 tcatgaactg caaagtatat acataccatt gccgctgctg catctgctat tggtcctgca
      241 aacattactc catcaattcc cataaacatt ggtaaaatta ctatcaatgg aattaaaaat
      301 aaaatttgac gagtcaaaga agtaaaaagt cccaacactg atttcccaat tgatgtaaag
      361 aaagttgatg atactatttg aacaccattt ataaatgtta agaacaagaa agttctaaaa
      421 taatgttctg caaatataaa ataagtctca tttccgttac caaatataga cgtaatttgt
      481 cttgggaata attggaaaca taagaatgca ataacagata taatagttgc acttcccact
      541 gcaaacttat atgcttcttt tacacgatta tacttttcag ccccataatt ataccctata
      601 attggttgca taccttgtga tataccaata attatagata taaaaatcat atttacttta
      661 ataatgatac cgacacaagc aagtggaatc tcacttccat aaactgacct agccccataa
      721 tatgccaatg tattattcat aactatctgt gttacagtca ttgctaattg attaaaacat
      781 gagcttgaac ctagcgcaaa tatatgttta taaattatac tatctaattt aaaacttttc
      841 ttatgtaatt taatatgttg aaaattcttt atataatgaa cacttattcc aaaagatata
      901 agttgcccaa taacagttgc taaagcagac ccagcaattc ccatattaaa aacaaaaatg
      961 aataatggat tcaaaattgt attaataact gctcctatta acatacaaaa catagaagct
     1021 ttcggtctcc catctgcaag tatcaactta ctcattccag tggacattat aagaaatggt
     1081 aatccaattg atgtaatttt tgtatatgtg tgtgcataag gtagtatttc tgatgtagag
     1141 ccaaagaatt ttaatagtgg tgttaaaaat attgatacta ctataaataa acttattcca
     1201 aaaacagcca tcaagcatat agaatttcct gctgcttttg tagctttttc tgaattttta
     1261 gcacctaatt ccaatgaaca cttagacgct cctccaattc ctaacaataa tgcaattgca
     1321 gtacatattg ttgttagtgg aaaagtgaca tttgtagcag cattgcccaa tacaccaaca
     1381 ccttgcccaa taaatatttg gtcaacaatg ttatataaag cacttactaa cattgctata
     1441 atactaggaa ttgcgtactt agttaataac tttgaaacct tttcatattc caatggctcc
     1501 ttatattttt cttccatttt ccttcgcctc caattttgct tcttcattca tatcaatagc
     1561 caaaaatgca actttcttta acattgacat aaacaactct tgctcttctt tctcaaatga
     1621 actcaacaac tcctcaacat acccatttgt tatttttagt atttcatcat aacatcctaa
     1681 agatttttct gtaggatata aatgccatgt tcttttatcc tttacacctg gttctcttgt
     1741 tataaagcct tctttttcta aatttgccaa agctcttgtt atattactag gattcacaag
     1801 aattttctca aatatagtgt cctgtgtaat acctggatta ttacatatgt gtaatacata
     1861 aaaatattgg ctattattga ttccaatatc cgccaatttc ctgtcaatat aatttgtatt
     1921 gtatctattt ataatggaaa cccattttaa tagctctctc atattctctc ctttaatatc
     1981 tattgtcctt tttttgcgtt cgcaacaatc tatttgctca tgcaattatt ctactgctaa
     2041 ttttatactt tgtcaatagc acacaagttt atatttaaac cataaagtta ctataaattt
     2101 tatatattag actcttactt tgatttaact atttttaaat ctttattgat atttaattac
     2161 cttttatatt aatttttttg catatttatc aataactcct aattcttttt agcttatctt
     2221 aaaaagcatc ttgacgtaag atttattatc tgtaataata aatatatcga atcgatatat
     2281 atctaattga taccaggagg taatatgtta gaatacatta ttttaggatt tttaatggaa
     2341 aaagaattaa gtggatacga cttaaaacaa ataatgtcag aaagtacctc gtactttttt
     2401 gacgccagct ttggaagtat ctatcctgca cttaaaagat tagaaacaaa aggatatata
     2461 cactatcacg aagttataga tggtaacaaa ttaaaaaaac tatattccat aactaatact
     2521 ggtaaagaag tatttcttga atggttaaag aaacctataa atttttctaa aacaaagcaa
     2581 gaccaccttg ttaatatatt cttttacaag tatctaccca aagaaataat tgaaacaaac
     2641 ttaaaaatgt taataactga agtagaatta cttctaaata aattaagtaa acaaaaaaat
     2701 gatgcagaag aaaatcattg tgttgaccaa tatacatata tgtatgcaac aatgatttat
     2761 ggagttgact attataattt tgttattaat tggtgtaaag atttactgaa aaatctataa
     2821 gatataattt ttagaagtaa acatacataa taaaaggaga tggactttat gaggctttta
     2881 atacatgatt taactgacaa agaattagaa aagttattac ccaaacttga tgacaatata
     2941 aaaatacttc caaacacaaa agaaatccac aaatgtattg gttgttttgg ttgctggatt
     3001 aaaactcctt ctgaatgtgt tgtaaaagat tattacagca acatgggtaa acttataaat
     3061 gactctgatg gaattattat aataagcaga tgctgttacg gagggtttag cccttttgtc
     3121 aaaaacgtac ttgataaaag cattaattat attcttccat actttgtaat aaaagataat
     3181 atgatgcgtc ataaaagcag atatgataaa cagataaatt taaaagctat attctatgga
     3241 gattgtatta cagaagctga aaaagaaact gctacgaaat tagtaaatgg aaatgctata
     3301 aatttcaata taaaagaaca ctctgtttgt ttctatgaaa atatacaaga tataagaggt
     3361 gaatccatat gaatatagta atgataaatg gtagtccaaa agtaaataaa aataattcac
     3421 agtatttttt atctgaatta aaacaactta ttcaaaatga taataaagtt tttacattta
     3481 aagtagataa cacatctaag tatcctgacg ctcttaatgc catacttaat tgtgatagat
     3541 taataatcgc atctcctctt tatgtagatg gtattccatc tcatttactt cattttttag
     3601 agaaatgtga aaaattttta aaaacacaaa ataaaaaacc tattccagtg tatgcaatag
     3661 ttaactgtgg attcaatgaa ggtgaacata atcatttggc aattcaaatg atagagcatt
     3721 ggtgtaaaaa aatcaacttt acatgggttc aaggtattgg aattggagct ggggagatgt
     3781 ttggttcact agaatccgta cctttgggta aaggacctaa aaaagattta ggaattgcat
     3841 taaaagaact ttcaaaaaat atacttagtg ataatataca tatagatgat tccaacaaaa
     3901 gtactatttt tatttcacct aacttcccta gattcctata taaacttatg gggaatcatc
     3961 aatggaatac tcaagctaaa tcaaatggat tgactaaaag tgatatacta aagaaataaa
     4021 attttacttc aacaaatact aaaaaccgtc aagcacgaat aaactattca tcttgacggt
     4081 tttttatgtt atttatattc actagacatg ggataattca ctataacatt gattatctag
     4141 tctaatattt atcttattct ttttgtaaaa tccaagttgc tccaacaagg tcctgctgta
     4201 aatcagtata ttcatttttt tggctaataa catattgttc aaacatatct ttttgagaag
     4261 gatatttttt aattagctca tttgcacgag ttataatcca attcatttcc tcttcaacat
     4321 cattttcaag ttctaaacat ggtatacatt caattagctt cagacctaat tctttgaaca
     4381 atatacacca ctcattgtaa gtgggataca ccctctcaaa attaagctgt tcatttgtag
     4441 atttatctac tacatatgca tcatcaatta aaatgaaccc cttacttttt attgtaggta
     4501 atagcttact tatagtttct ttataattac ctaaaatatc ccctacagct ccaaaaatca
     4561 cacaatcata atcatgctca atttttacag aactattagc atcattcatt tcaaaatcac
     4621 acaaagcatc aaccttatat tcaatagcct ttcttctagc ataatcaata aattcaggta
     4681 tcaaatcaat acctttgata tgtatattca actcttttgc aagcttcaca gatacagcac
     4741 cttttccaca agctaaatcc aaaacttttg tatcctcatt aaaaggtata tgctttctaa
     4801 ataattgtaa catttgttct ggtgaacttc ctaattccca aaaatcttgt aataaataag
     4861 gcaaataagg taaaatatca gtagtatcag aagttagtga ctctgccaat ttttctttta
     4921 cagtatcatt cattttttca acctccaaaa tttaaattat tattcaaaag taatattatt
     4981 ttatatactt cttcataggt actacaaaca gtggaaaatg cttttgacct ttgtattttg
     5041 caattcattt cttttcttta tgagctgtaa taattgtata actgtgagcg tttactgtta
     5101 taatacaatc atatacactt atataccagt tttttccctt tctattaata atggcatttg
     5161 gagattctat tttagtttta caccagttca ctacatcatt tgtctccaaa gaaagatttt
     5221 tcttaattct tacaacgcct aactctgttg tatgcaatct atctaggttt ttaagtaatt
     5281 cgttttctac attcattttt attaaaacac cttatccttt gcaaaattaa atctattatt
     5341 tagtactatt ttatcatagt attgtgtatt ttatcataat cttatagtaa aactatttct
     5401 ttcctacaga ttataaatta tttatgaata cacaccatta tagaactttt cctatgcaaa
     5461 attaaatcta ttatttaaca ctattttgtc atagtattgt gtattttatc ataatcttat
     5521 agtaaaatta tttctttcct acaaactata aattatttat aaatacatac tgctgtaaaa
     5581 ctttgcctat ataaatccat ctactttata cataatacct tcttttaaat gattggaacc
     5641 caaaactgaa tttcactcaa accattctca tcagtttttt ctccatattt tacaaagtca
     5701 tataaattat tttcattaaa ctggcaactt gaattaggga accattcttg atagatatat
     5761 tctacaacat tttgcatact atcctcattt ttcccaataa aagtaaaaac aatataatta
     5821 cttttcggta atacgaattt ttccattcca ttaggtaatt tgccaaaagt agctacttga
     5881 gcacctgcaa tgtatgtaaa tgtaggagta ttctcaatct ctttaaaatt gctataatca
     5941 ctaactccaa ttaaaaaacc taaatctacc ttattttcta tcatctcttt atttttatgc
     6001 aaatccctcc agcattttcc aataatatga aatccttttc ttactttacc agaatagcca
     6061 acaatattta tatctgcaca attaactttt ttaacatcta aaaccatatc tccacgtaat
     6121 ttttttctat tatgaatatc aaattttaat tgtagtggta aaaaatcttg atgtcttcta
     6181 aaaaaattag gactacgttt aaacattttt ttaaaccctc ttgaaaaaga gcgttgtgta
     6241 tcataccccg agattaaagc aatctcaata attggttttt ctgtaaaaat tagcattctc
     6301 gctgcttcag taagtctacg tctttgtata taattatgta cactaaatcc gacaactgat
     6361 gtaaacattc tatgtaaatg gtattttgaa tagcctatct ctttagaaat tacatttaaa
     6421 tctaacatct catctaaaag atgtttttct atataatcta aagttttttc aatggtaatc
     6481 aaattgttat ccatatagca tccctcctat tttaaagata gcatattatt ttattttgtt
     6541 tttcacattc tttgctaata ttttgattta ttttatggca cacaaaagac acttctattt
     6601 gaagtgccta tctactctgt atattgagct tcttatttca atttattata atcttcaaca
     6661 gatacaggtt ctagccattt attgaaagca ccttcagcag ttacctcgtc tacagccaac
     6721 tgctaaatgc ccttctatac cttaatctaa cttttccact tcaagtatac aacttgaagt
     6781 tagctctaaa tcaatagttt ttatcacaac aatttgattt taaatttaaa tttattcttt
     6841 taaattatca tcatctatta tagattttct tatattcaat aactggtcta caaattggtt
     6901 agcaggagta tttaaaatat tctcaggagt atcatactgt tgaacaactc catgatttaa
     6961 taccaaagtc tttgttccca aaatcaaagc ttcataaata tcatgcgtaa taaagataat
     7021 tgtaactcct gtttttttat gaatttcttt tatctctttt tgtaattgct ttcgtgtaat
     7081 ctcatcaact gcaccaaaag gttcatccat taataaaatt tctggagaag aagctaaagc
     7141 tcttgcaatc cctacacgtt gttgctgacc acctgacaat tctgatgggt aacgcacttt
     7201 catatcattt ggcaatccca ctatttccat ccacttgttt acagcacttt ttacttctaa
     7261 tttattcttt ttacttagga gatttggtac atatgaaata ttttcttcaa cagtcatatg
     7321 aggaaaaaga acacttccct gtacacaata tcctattctt cttcttaatt taattaagtc
     7381 ctcgtttttg atatctgttt ggtttatcaa aatatttcct ccatctggca aaatcagacc
     7441 attaatcatt tttaagagtg ttgtcttacc agaaccagac gaaccaatta cagtcaaaaa
     7501 ctctcctttt tctatatcta aattaagatt atctattata gttttatcat tatattgttt
     7561 tttaatattt ttaaattgta taataggtgt cattatattt cctccaattt ataaaagacc
     7621 tttcttattc agaaactcac gagcaacttc tctaggttct aaattgttgt tttctacgtc
     7681 ataattcatt ttagccattt ctttatcagt caaaatatct ccaaccatct taaatacctt
     7741 tgatagttct ggattttcct ctaatacttc caaacgaaca acatttccac attgataaga
     7801 tgggtagaaa tttttatcat cttttagaac tacaacatct gaaacactat actgaccatc
     7861 agttgtaaat ataggcatta cgtcaatctt accttcattg atagcttggt attttaatcc
     7921 aatatctaaa tcaactgtat ctttaaagtt cagtccatat gtattacaca atggagtata
     7981 tccatcctct ctctcataaa agtcatattc agctccaaat gtcaatttag aagatacatc
     8041 ttttaagtcc gttatatttt ttaaattgta tttatctgca acttcttttc taactgctaa
     8101 tgcatagcta ttagaaaaac catacatacc agtccattgc attttaagtt tagttttgta
     8161 ttccgactgt agtttactaa acatactttc agtatataat tcatttttct taagtaccat
     8221 attccagcct gtacctgtat actctggata aatgtcaaat tctccattct ccatagctgg
     8281 ctggatatta gatgttcccc ctcctacacc ttgtgtaatt tccacgttta gacctgtgtt
     8341 tttttcaatt aaaatatcca acatctcacc aagaatatat tgttcagtca taggtttagt
     8401 agcaatatga atagtttgag tctttgaagg attaaataaa taagacgtaa agatagttgc
     8461 tataagaata aggcttccta gaatggcaaa aacttttttc ctatttttct tttttctttt
     8521 tcgttgaaaa aatttttcta ccagccctaa aataaaatca aatattaaag ctaatagagc
     8581 aataagtata cttccagtaa ttgtcattat tgtgttattt gtcgtgattc ctctatatat
     8641 ggctacacct agacctccag cacctataaa tgaagcgata ccagctaatg caattgtcat
     8701 aatcaccata tttctaatac cagacataat aacaggtagg gctaacggaa gttttatttt
     8761 atacaatatc tgaaaatctg tacttcccat tcccctagct gcttcaagaa tatttttatc
     8821 cacattagta attccagtat atgtatttct aaccattggc aataatgcat aaatggtaag
     8881 tgcaataaca gcagtagtat ttccaattcc ggaaaatggt atcagaaatc ctagcataga
     8941 aatagatgga atggtgtata gaaaattgat agttcccaat ataggtttag aagaattatg
     9001 aaattcactt acaataatac caattatacc acccaaaaca atcgcaatta ttatagaaat
     9061 aatagaaatc atcaaatgtt ctatggatag ctttataaaa aaatcacttc tttcagataa
     9121 taaggttaga atatcattta tcatccatct ctctcctttt ataagtttta cttaacaaga
     9181 tttaaatttt aatgtagtta atctttattg tttatacatc agcaaatcgt catcaaatta
     9241 tttcacaaat tatatattaa gattataatc tgaacctccc atgactgaag tcacaagtgt
     9301 gtatagccat tttttaatca aacttgcaag tagacacaaa aaagtaacta ttagagactc
     9361 catcctcgat attctccata aaaataagaa aaaaggtcag aaccgttaaa atactaaaat
     9421 ttaaacaaat ttctgacact ttatttaatt aaatttgaat atttctcaca ttatttatta
     9481 ctgttaagtt taattatcat tttatatacc cattatattt attaaaatat cattataatt
     9541 ttattatatt ttatttatta tactatatat tttgctgtta atgatatttt attgttacat
     9601 aattgttttg gaataccttc aaatattact ctcccgcctt ttgttcctcc ttcaggtccc
     9661 agctcaataa tccaatcagc tcttttaatt acatctatat tgtgttcaat tattatgacc
     9721 gtatttccat tatctacaat ctcttcaatt atacttataa aattctcaat atcagccata
     9781 tgaagacctg ttgttggttc atctaatata tacactgaac tttccttatt taattcacta
     9841 gcaagcttta atctttgaca ctctcctcca gacaaagtat caagagtttg tcctaaagta
     9901 aggtatccaa tacccatttt ttcaatacat tgtaatttcg ttttaatctg ttttaaatta
     9961 aaaaactcaa ttgcctctaa aacagacatt tcaagaactt caattatatt cttcccttga
    10021 aacctatatt ctaaaacttc ttttttaaat tttttacctt cacaaacatt gcaggtactc
    10081 tttatactct ccataaaagc aagattcatt tctactattc cagaaccctt acagttatca
    10141 caagctcctt ctgaatttga actgaataaa gaagcattta cgccatttgt ttttgaaaat
    10201 atgcttctta tgttgttcat aattccacta tatgttgcca aactcgacct tgaatttgct
    10261 gaaacagggc tttggtctat aagtacagca ctaggatttt gctttaaaaa ttcatcctta
    10321 atcaacgtac ttttacctgc tcctgctact ccagtaacta gagtcaatac acctttaggt
    10381 atcttaatcg atacattttt taaattgttt ttattacaat ttttaacttc taaatatcca
    10441 ctatgttctc ttacactttc tttaatagga agaaatttac ttaaagcatt ccctgtcaaa
    10501 gtaccacttg taagtagatt ttcataactg ccttcataca caacttctcc accatactta
    10561 cctgcctttg gtcctacatc tacaatatgg tctgcaatct taataacatc tgggtcatgt
    10621 tcaacaacta taattgtatt ccctttatca cgcaattttt ttaaaagatt atttaattta
    10681 tacacatctt ttggatgaag tcctacactt ggttcatcaa aaatatacat taaatcaaca
    10741 agattactat ttaaatactt aaccatcttt atacgctgag attcccctcc tgatagagaa
    10801 gatgtctctc tatccagtgt taaatatcca agacctatat ctatgatact gtttaacttt
    10861 tctattattc ctttaattaa aggttgtgct tctggctcat ctatactttt aataacttca
    10921 gccaattcat caatttgcat actagttaaa tctgctatat tataaccatt aattaaagac
    10981 ctatatactc tttcattaag tcttcttcca tcacatatag gacatctatc ttctataaga
    11041 agtttactca attttttctt agtagcttca gatatttctc cctctctact taagtatagg
    11101 cgattaaatt taaccattaa tccctcatat gtcatgttag tttgaccagc attttctatc
    11161 ttaatctttt ctgctttacc atgcaaaaac ttttccaatt ctacttctga ataatcacat
    11221 atcctcttat taatatcaaa aaatcctgat tcagcgaata tattccaatg ccaagaaccc
    11281 actccaaacc ctgaaagcaa tatagcacct tcatttaatg atttattcat atcaagaatt
    11341 tggttcatat taggaactag cttttttcca agcccatgac agtgtgggca cataccatca
    11401 atattattaa aagaaaacat attagcatta cctatatact cactaccaaa ccttgaatat
    11461 aaagctctta aaaaggaatt tacatctgta atagtaccta gggttgacct tgaatttcca
    11521 ccaagtctac tttggtctat gataattggt gttgacaaat tttctataca atcagcttta
    11581 acctctccat acttaggtaa aaaacttctt acaaatgtac taaacctttc atttagttgg
    11641 cgttgtgatt cttttgcaat tgtctcaaat acaatagatg actttccaga acccgacacc
    11701 cctgtgaata tggttatttt tctttttggt atttttaagg aaatattgtt caaattgttc
    11761 tctttgcaac cttttaaaat tataaattca ttgttcatat tttacctcct gcattatttt
    11821 tctttaaatt atttagagaa aatcgtaata atagttgtta gttatacata agtgaaatac
    11881 tattttatta gattactcct ttttttataa acacaggtca aattatagca tgtagatttt
    11941 atctggtagt atagtgtagg gtttgatttt tataaaaaat acagttattt tatttaagcg
    12001 taattgcatc tattctacct aaatatcatc attatatatg agtgttaaat tatataaaat
    12061 tcttaagtta ataatctcaa aatggtatta gttacataaa aaaagtatct tgcctatgat
    12121 tttaaaacca taaataagat actatgtttt acactgtttc ttctttcaca aataattcta
    12181 tacaattatt caacttaact ttaaaatttt aaacactaaa aacctctatc tttaaatctt
    12241 taaataaaac taaatttcca ataccttcaa aactatagaa aattccaatc aacttatcaa
    12301 acataactta ctacttataa gaaattaatc ttcacttaat acagacgtaa gttcagggtc
    12361 atttagaaga tgttcaaaat aactatgtgc cgaaatatat cctataccat acattttacg
    12421 ctttgaattt acaaccttat attcataaca agcactctta accttattgt agtcatcaaa
    12481 atccattgct ttatagatag ctctttcatt tcttggtata tactttacat attttggctt
    12541 aacaaaatac aagtcatctt ttatcttacc ttcacgttta agtttcttta aaacttcccc
    12601 attctttcta tgcattatat ggtcatcata cttatagtga tgggctatat gggtcacatt
    12661 gcctttaaac ttatgttcaa aatccaatat agtcttttcc attaattcat aatgtgtgcc
    12721 ttccttttta tctatatctg caagtataat cacattttga ggtttcactc caagctctct
    12781 caaagctgat ttaaactcgt tatttctaag ttcttccttt tgcttcctag ttaaatttct
    12841 aaattttgtg ttagctttga aaacgttaac tccagaacca tctgatacta ataccacata
    12901 tacattatca acaccacact gttttatagc atctacaatg gcacttcctc cccataaaac
    12961 ctcatcatct tgatgttgag gataaaacac tgctgctgat ttaaattttc tactattact
    13021 ctcaggaata ggtatttcaa gttttccgtt aaaatttttt gcaattaaaa atatcatcaa
    13081 taatattaat atacttattc ctatgtactt cgttttactt tccatattta aaattttctc
    13141 cttcttctga acctcccatg attaaagtca cagggttact acttcataga atttcgcata
    13201 atcaactcaa ataccttata aacctttgtg ccaatcaggt ctaaatatac aaattatttt
    13261 acaatatttt attgtttcat acaatctttg acacgaacta taataaaata aaaaagcctt
    13321 ctaactgcaa cattgcaaac attacagcca gaaagcttct taattcttaa aatttattaa
    13381 gcttaaaaat ttattaaact tattttttaa tataaatatg tctattatta ataaattata
    13441 ctttaattac tatatcaata cgactttact atatcactat aacattactt atatcaatac
    13501 aactattttt atagtctaca tttcctttaa aatttcaagc aactcttctt cactatacat
    13561 atatttctta ttacagaact ggcaaccaac ttctgctttt ttatcttctt ctattatttg
    13621 agccaatgat tttttaccaa ttgcaaccaa agcagttttt actctttctt ttgaacattc
    13681 acacataaac ccaacttcac aggtatcaag tattttaggt tccaaaccat ccaaaacaat
    13741 atttaatata tcctctggtg attttccttc ttttaacatg ttagttatag aaggtaattt
    13801 agctacattt tcctctaact tagcaatagt ctcttcttct gcatctggca ttaattgaac
    13861 tataaatcct ccagcaaact ctacttcatc ttcttttgta agaacaccta aagctactac
    13921 tgatggagtc tgctctgata cagcaaaata ataagtaaag tcttctgcaa tttctccact
    13981 taccaatgga taagtcccat tatatggttc tctaagacct aagtctttta taacttttac
    14041 aacaccttct gtacctactg ctgctgctac atttaacttt ccatttggat agtcttcaac
    14101 ctctacttgt ggatttccta catatccttt taccattccc ttagagtctg atgtggcgat
    14161 aatagttcca attggacctc caccttttat tattacagtc aatttatcac tatcattttt
    14221 catcattaat cccattatag atgtggctgt taatgttctt ccaagtgctg ctgttgctac
    14281 ttttgtagtt tcatgtaatc ttctagcttc ttctactgta tttctagtag ttgcgacaaa
    14341 tgctctaact tgaccatttc ctgatgtcgc tcttagtaca taatctctca ttaatattcc
    14401 tccatgtccc gttaattgtc ttatttttat tatattacta ccttaatttt aatctataaa
    14461 tacttatcta tcttaacctt gggcaaaacc cattttattt taattttact aagcaatagt
    14521 tattatattt taatttttat ctatagaact ttataattat taaaaaataa aagtcataaa
    14581 gtatcatcac tttactttta atctataaaa ctgcattttc tatattttta tatcatggtt
    14641 atttttaata attatcttat atagtcaaaa atatcttatt ttttacatac aaagaagatt
    14701 ctttgacttt cttcactagg tttgtcaaat gtaaaatccc cataatattt tatatcctta
    14761 aatccagatt tttcaagcat atcaactatc tcatcttcag tatatgctct ttgttggtgt
    14821 atctcttcaa atctttcaaa ttttccacat tcatctttaa taaagaatgt aagttccatt
    14881 tcaattagat tctcttcttc atcaaagtaa ttttgccaca tatacgctat atcttctcta
    14941 ttttctccat acatgttatt tcccaatatg ttggctaatt tataaaaaga acttatatca
    15001 aatatgaata ttccatcttt ctttaataat tcatatgtct ttgaaaatac attttccaaa
    15061 tcatcatcat aagttatata attgaatcca tcacaagcac aaagtacaca gtccaaatca
    15121 gatatttcaa aatcaagctc tgatatatct tgttgaagta aaactaattc tactccttct
    15181 ttttctgact tttctcttgc tacactaagc atctcatcag atatatctat accagctata
    15241 tcgtaatttt ttttagtcaa tggaattgta agattacccg tgccacatgc caattccaat
    15301 atatttttta cttttacacc ttcactatct attatatctt ctatatattt aacccaacca
    15361 ttataatcaa cttcattcat aagttcatca tatacaaacg caaaatcact atattgactc
    15421 atccttttta ttctgctcct ccacaatctt tttaagcctc tttttgcgtg atgtaagcat
    15481 acctccaatt cttcctgctt ccttagctgt aagtcctgcc catccacgtt caccaacttt
    15541 atccataaga cctagttctg aggctatctc atatttcatt atatcatcat ttgtcaatat
    15601 tttagtattc ttgccattag ttatcttctt ttctctttgt actttttctt tcaaaaaaat
    15661 caccccttct tatttgtatt tttgtcaatt aaaataaatt tatacaaata tgtaaggggt
    15721 atttattaat acactttttt taaatttagg tatttatgct ttttaatctt tataacttat
    15781 cttcctaatc tattgcttct ttgtatttta ataattttga ataaacattc aatgcattta
    15841 cacatatctt ttcatcaaaa ttaaagttta tattatgaag accatttaca aatccttttt
    15901 cttcgtttct tgaacccaac ataaaaaata atcctggaac ttctttttga taataagaaa
    15961 aatcttctga tatcataagt ggctctaact ctacaacagt atcacttcca atagcctcta
    16021 caaactcttg atacaaatcc ttgtcatttt taacagcgat ataatcatct ataatattta
    16081 tattaacctt acagttataa gccaattcaa accctctagc aagttctcta actctaagct
    16141 tcattttctc atacacttct gggctgaaac atctcatagt accttccatc tttatatttt
    16201 cagctattat atttcttcta gctccccctt caattctgcc aatagtcaac actgcattgt
    16261 ctattgggct tatatttcta gacactatag tttgaagtga gcttacaaag tttgaagcta
    16321 tcactatacc atcaatacca ttttgtggca tagccccatg tccactttta gacactattt
    16381 ctatgtcaat atctcctatt tgagccataa aatagccctc tcttgtacct acataacctt
    16441 ctggtaactc tggatatata tgaagcccat atatttcatc aatattgtat tttctaagca
    16501 ctccttcatc tattaaagct tttgctcccc caggaccttc ttctgccggt tggaatataa
    16561 atactatatt atcatttaag tcttctttat tatcatttaa atactcaatt aatccaagta
    16621 agatagtagc atgtccatca tgaccacata aatgttttac cccttcttca ctatgaagac
    16681 catctatgtc agctctaaaa gcaatatctt ttttaggtct tttaccttct attacaccta
    16741 ctatacctgt ttctaaataa acttcatact caattcctaa tttatcaaga taatctctta
    16801 tatattttga agtcttatac tcttttctcc caatctctgc aataggattt aatgacttcc
    16861 tatgtttata aagttgttct tgtaaatatg atgcctctct catgtaatct cctccaattt
    16921 ctaatttatt aaaaaatatt tctatatttc tttataatta aaatattata tagcatgtta
    16981 tactaattaa aaataaaaag ctaaattgaa tataaaaata ttttgcatta ttaaataatt
    17041 caccattaat aataattccc ctgtttcata aatcaaaaca gaggaattat tattggaaaa
    17101 ttttattttt agcaagagaa gaaatgttta gcaagtccac ccttagctgt ttctttgtac
    17161 ttatccatca tatctatacc agtttctttc attgtttcaa caacttggtc taaggagaca
    17221 gaatgtgagc catcagttaa taatgcataa ttagctgcat cataagctct ttgagctgcc
    17281 attgcatttc tttctataca tggaatttga acatatccat atacagggtc acaagtcatt
    17341 cctaaatgat gttcaagtgc tatttctgcc gcatattcaa tatggtctat agttccacct
    17401 tttaagtatg ctactgctgc tgctgccata gaacaagcgg ctccaacttc accttgacac
    17461 cctacctctg ctccagatat tgaagcgtta gtttttatta tattacctat tattccagct
    17521 acaagtagtg cttcaattat tttttcgtca tcataattat agaaatcctg cattgcaaag
    17581 aatattcctg gtattactcc agcagaacca caagttggtg ctgtaactat tatattacca
    17641 gaagcgtttt gctctgaagc tgctagtgca tatgcatata caagtgttga gaagctctta
    17701 tcttttttat atgcattata aaatgtactt gctcttcttt ttaataataa cttgcctggt
    17761 attatttcat ctgtacttag tccatcatca atagattttc tcatagcatc ttttatagtt
    17821 tttatataat cttttatgtc ttttggttca cattccaaca caaaatctac caaagtctta
    17881 ttattttctt tgcaccattt tactatctca tccatagtat ctaagtgata tatactattg
    17941 cttttactgt ttctttgacc ttcttctgct atagttcctc cacctacaga atatacagtc
    18001 cattcagctg ttacattttt atctttatct aaagcttcaa acttcatccc atttgggtga
    18061 aaatctttta taatatcttc ttcccaaaca atttcgactt ttttaggtgc tatagttttt
    18121 tcaattatat agtcagttaa gtgacctttc cctgtagctg ccaaactacc atataatatt
    18181 gctctaaaac tttctgcatc tgggttttca ttcttaaatc tctctgctgc tctttgtggt
    18241 cccatagtat gagaacttga aggtccactt cctattttaa aaagttcctt taaagtatcc
    18301 atattattcc tcctaaattt acggtattat tgcttattgt tagattttac gcacatattt
    18361 attataacct aaatatatta attattagtt attaaattct cataaaattt attttttatt
    18421 tccattttgg tatattttat taaattaata tgcatatttt gagaataaaa catttattta
    18481 aaatttttct aatattaata taatataaat atcttattta aacagttaag atattaacta
    18541 tacctattaa aattaattaa tttgtactat aattattatt gtacttaaaa aattaaattt
    18601 agataaaaaa ggagatttga ttatatgtta tttaaaggtt ctgctgttgc tttagttacc
    18661 ccatttacag aggataataa tgtcaacttt gaaaaattag gtgaattaat cgaatatcac
    18721 attgagaatg gcacagatgc actagttgtt tgtggaacaa caggtgaggc gactactatg
    18781 agtgaatctg aaatttttgc tgtaataaaa tatacagttg aaaaagttaa taagagaata
    18841 cctgttatag caggtactgg ttcaaacaat actatgttgt ctgttcatat gagtcaagaa
    18901 gctgaaaaat taggggtgga tggtctttta ataataactc cttactataa taagactaat
    18961 gagaaaggtc ttaaacttca ctttgaaaca atagcaaata gcgttaagtt gccaattatt
    19021 ttatacaacg taccaggaag aactaaagtg aatataaagc catctgttgt ggctgaactt
    19081 gctaaaatag ataatatagt tgctgtgaag gaagcaagtg gagacttagc tcaagttgca
    19141 gaaatagcta aattagtacc taaagacttt gctatatact ccggtaatga tgatactata
    19201 ctaccactat tatcattagg tggaagtggt attatctctg tacttgctaa catatgtcct
    19261 aaagaaacac atgatttagt aactaaattc tttgaaggtg atattgaagg ctcaaaaaaa
    19321 ttacaacttg atatggatgc tttaattgct gctttattta tagaagtaaa tccagttcct
    19381 gttaaaactg ccatgaacat tttaggtttt aatgttggtg atttaagact tcctctagct
    19441 gaaatggaag aaactaatct taatgtgtta aaacaagaat tgacaaattt tggatttaaa
    19501 ttttagcaat atttatataa ataagttgat tttttcttaa atatgtatta ttattagaat
    19561 aatcaaattt taatatccta ttaacctagg gtagaggtag ctgtaactaa gagtatccaa
    19621 tagtagctgg caggtgttaa tgattggaaa aaggatttac agccgaagaa aatatttctt
    19681 gcaataatat tttctgggat tgcacataac atgtgtaata ctgtcactta tttttgtagg
    19741 tgatgagcta cagggtttac tttgagtgat aaattagacg tacaaagtat acctttgtac
    19801 gttttttgta tataattaaa gttgttaata taatattaaa gtttttggat atataaaaat
    19861 attaacatat cttacttgta taactaaaat aaaataatgt tttaataatt taaaattggg
    19921 gtgaatttat atgaaaaaag taaatgtagc aataattggt gctactggaa tggtaggtag
    19981 aacattttta aaagtattag aagaaagaaa ttttccaata gaaaatctat ttttattctc
    20041 ttcttctaaa tcagctggtt ctaaagtaat gttttgtggt aaagaatata tagtagaaga
    20101 attaaaggaa acttcattta ctgatagagg catacaaata gcattattct ctgctggtgg
    20161 aagtattagt gaaaagtatg ctccaatagc tgcttcaaat ggaatcttag tagtagataa
    20221 ctctagtcaa tggagaatga atccagatgt gcctttagta gttcctgagg ttaatccaga
    20281 agccataaaa aatcataagg gtataatagc taatcctaac tgctctacta tacaagctat
    20341 ggttcccctt aaagctcttg atgataaata caaaattaag agagttgttt actctactta
    20401 ccaagctgta tctggttctg gtgttaaagg tgttgaagat ttagagaaag gaatgaacgg
    20461 taatcctact ggattctacc ctcatcaaat ctcttacaac tgtcttcctc atatagattc
    20521 attcactgaa aatggataca ctaaagaaga aatgaaaatg gtaaatgaaa ctatgaaaat
    20581 acttgataac tacgatttaa aaataactgc aactactgta agagttccag taaaaaatgg
    20641 tcatagtgaa tctataaatg ttgagtttaa caatccattt gagttagatg atttagtaaa
    20701 aacacttgaa gaagctccag gtgttgtagt agttgataat cctgctaaaa acgaatatcc
    20761 aactgctgta gaatttgatg atagagatga agtatttgtt ggaagaataa gaagagattt
    20821 tagtgttgat aatggtgtaa atctttgggt tgttgctgat aatataagaa aaggtgctgc
    20881 aactaacgca attcaaatag ctgaaatggc tttatcatac gatttagtat aatatacaaa
    20941 cttgtataaa tatataattt tgtaaaatat taatagtaac tcaaaaacaa gcattagaag
    21001 taactgtaat ataaaacaca aaaataaaaa agaggggatt gattttatat gatatttaaa
    21061 ggttctgctg ttgctctagt tacaccattt acaaaggata acaaagttga ttttgacaaa
    21121 ttaggtgaat tagttgaata ccaaatagct aatggtactg atgctatagt atcttgtggt
    21181 actactggtg aagctaatac tatgactgat gaagaacaac ttgctactat taagtatgtt
    21241 gttgaaaaag ttaataaaag agttcctgtt atagctggaa gtggttctaa tgatactatg
    21301 cactcagtta acttaagtca agaagctgaa aaattaggag tagatgctct attaataata
    21361 acaccttatt acaataaagc taataaagct ggacttaagc gtcattttga aacaatagct
    21421 aacagtgtta aacttccaat tatactttac aatgtccctg gaagaacttg tgtaaatata
    21481 agtcctagcc ttatagttga acttgctaaa attgataaca tagttgctgt aaaagaagct
    21541 agtggagacc taggacaggt tgcagaaata gctagtttag ttccagatga ctttgctatc
    21601 tactctggta atgatgatac tatacttcct ttattatctt taggaggtca aggagttata
    21661 tcagtacttg ctaatgtttg ccctcaagaa actcacgatt tggtagctaa attctttgaa
    21721 ggcgatattg aaggttctag aaaattacaa cttggtatgg atgctctaat cgcagcttta
    21781 tttatagaag taaatcctat accagttaag actgcaatga atttattggg attcaatgtt
    21841 ggagatttaa gattacctct agcagaaatg gacccagcaa atttagaagt attaaagaaa
    21901 gaattagtta actttggact aaaagttaat gcatagtaaa gattaatgca tggtaaaaag
    21961 taagtttggt caaaagttag cccttaatca taggaggaaa ttatgttaaa agttatagca
    22021 actggttatg atggaaaaat gggtaagatt ttagccgata ctataagaga agataatgaa
    22081 ttagaattgg tttgtgttgc agctagaggt ctggatagct atgaaggcga ccttaaaatt
    22141 tatgaagata tgagtactat agttgaggaa gctgatgtag ttatagattt ttctcatcac
    22201 tctaatttag ataatatctt atcttacgtt ttaaagacta aaacaccttt agtaattgct
    22261 actactggat ataatgatga cgaaatgaac aaaatacgtg aagcggctaa agttattcct
    22321 gtattccaat catataatat gtctcttggt gtaaatgtat tagtaaaatt ggttaaagat
    22381 gcagctaaat tactagaagg atttgatata gaaataatag aaaaacatca caacagaaaa
    22441 gtagatgccc caagtggcac agctgttatg attgctaacg ctataaaaga ggttttgcca
    22501 aacgttgaaa ataattatgg tagatatggt cgtagtgcaa aaagacaacc aaatgaagtt
    22561 ggtattcatg ctataagagg tggaaatatt gttggcgaac atgatgctct atttgctgga
    22621 gacaatgagg ttcttacaat aagccatcaa gctcaatctc gtggtatctt tgcaagtggt
    22681 tctatagctg ctgccaaata cttagttaag caaaaaccag gattttataa tatggacagc
    22741 atgttgggat aatagtataa atataataat acacaatata aattaaacat atacaatata
    22801 tatataataa tatgcaatac aaatcaaaca tatataataa atttatttaa tactatgtct
    22861 atacaataaa agctaactaa aataaaatat aaataataag aagtaagtat atactaaaat
    22921 tgacaaataa gtatataata aaagaaatat aatgaaatac aaggagaatt aacaaatggt
    22981 aaatttaaac gatgcttatg aaatagcaag gtatataaaa gaagttaaaa agaaaacacc
    23041 tgttaaggta tatgtaaata ctaactcttt acacttccaa tcaactgata aatttaaggt
    23101 atttggaagt aatggttctt atatatttgt aggtgactat gaggatataa tggaagtatt
    23161 agataaacat ggaaatgata taacagatac tcatgtagaa tatgatagaa gaaattctgc
    23221 tattcctcta aaaaatactt taagtgaaca tgcaagaatt gagccaggtg caataataag
    23281 agacatggtt actatagaaa aaaatgctgt tgtaatgatg ggagctgtta taaatatagg
    23341 agctgttatt ggtgaaggtt ctatggtaga tatgaatgct gttattggag ctagaggaac
    23401 tcttggtaaa aatgttcact taggtgctgg tgctgttgtt gctggtgttt tagagccacc
    23461 ttctgctact cctgttatag ttgaagatga tgttttaata ggagctaatg cagttattct
    23521 tgaaggtgtt agaatcggta aaggtgctgt tgttgcagct ggtgctgttg taactactga
    23581 tgtagaagct ggtgccgttg ttgctggttc tcctgctaaa gttataaaaa tgaaagatga
    23641 aaaaacggct gacaaaacta agcttatgga agaccttaga aatttagatt aattaaatag
    23701 tactatgcta ttaattaaac aataatatac taatttaaaa aattaattaa aaaggtgtct
    23761 ctgtgtgaat ttaattcacc agaggcaccc tcttttttac aatattttta tcttttattt
    23821 ataatcattt tattttttta caattctctt attcattctt atatcatttt tatttcttgc
    23881 tgacaagtat ttttatttcc ttcaattttt caatatatca atgtaataaa ctattaaact
    23941 ttttataatt taataaagtg aaaaccatta atctttatat aatttaataa aatgaactac
    24001 taaattttct atatatcaat agaataaatt attaaacttt ttataattca ataaagcaaa
    24061 ccattaatct ttatataatt taataaaatg aactactaaa ttttctatat atcaataaaa
    24121 taaactatta aactttttat aatttaataa atagaactgc tgtttaccac tggttgtgca
    24181 tctttattgc cacaaagcac aactaacaga ttacttacca tagctgcttt acgttcttcg
    24241 tccaaagtca caacattatt ttcacttaaa ttagtcaatg ccatctctac catacttaca
    24301 gccccctcaa caatcttttt acgtgctgct attatagcct ctgcttgttg acgttgtaac
    24361 attgctgctg caatctctgg tgcataagaa agatgagtaa ttctcacttc acacacttca
    24421 attcctgcaa tgtcaaccct tgattgtaat tcatctttta atctatctgc tatttcctga
    24481 ctactaccac gaagagattt ttcatctcca tcttcagata catcatatgg ataaagtctt
    24541 gaaacattgc gaatagtaga atcacactga atagataaga atgtattata attatccaca
    24601 ttaaatactg cctttgttgc atctataacc ttccaaatta caaccactcc tattataatt
    24661 ggatttccca ataaatcatt gactttctgc ttttcattat taagagtcat tgtttttaaa
    24721 gatacttttt tgccccttga attagttgat atatctacct tttcatcaga tgaagatttc
    24781 gatacattaa ttcttgatac tgctgggttt attgctgaac aaaatggatt cacccaatag
    24841 tacccttctt tttttatagt tccataatag ttaccaaaca aaactagaac tatagcttct
    24901 ttaggattaa tcatttttag cccaaaaaat aggataaaat ctactactat aaatacaagt
    24961 cccaaaatta acataagtga acctattcca gtaaatccac tatcaagttt tactatccca
    25021 aaaaccatga tacctattga aactactaaa aataataaaa tcattatcaa agctgccact
    25081 ccattgaata cttttgcgtc tttctcttga taagtttttt cttcttttaa catatctatc
    25141 actcccatct cagtatatta agttaagata tttctaattt aataataaca cttttaacaa
    25201 ttaaattgtt gaatttaaaa ttaatactat atgtttatat attttttata catctttatg
    25261 catacatgta tttagttatt ttaatgtatt aatattaatt tccaaataaa tcatatgata
    25321 tatgaatatg gtatttcaaa attaactttt tagttcatgg ggcactcttt ttggtatgaa
    25381 gtcaaatata tttttatcat ttaaacaatg aaaaagaaat ttatctatat tttgaaaaat
    25441 ccacttaaat tttttaatgt aagataaatt gtaagcttat tatcactaat attattttat
    25501 ataaattata ttagtgataa aataaactta tagaaatata acaaattttt attaatttca
    25561 taggaggaat ttatgttgaa agttataatt agtggctata atggaacaat gggaagagtt
    25621 ttagcagaat gtgtaaaaaa agataatgaa ttagagttgg tttgtggtgt agctaggtct
    25681 gctgatgaaa aaggtcatca tagtgataca aagctttaca aaacaatgac tgatgtaact
    25741 gaaaaaactg atgtaattat agacttttca catcatgata ctttagagcc aatattgtca
    25801 tatgctctaa aaaataatac acctgtagta ttagctacta ctggatatga cgaaaatgac
    25861 ttaaataaaa tagaagaagc atcaaaaaac attcctatat tattatcatc aaatacatct
    25921 cttggagtaa atatattaat aaaattagtt aaggatgctt caaagatgtt agaaggtttt
    25981 gatattgaaa tcatagaaaa acatcacaat agaaaagaag acgcccctag tggaactgct
    26041 actatgatag ctaatgcaat tagagaagtc ttgcctgaaa gtgaaaatac acatggtaga
    26101 tatggtagag attgtaaaag aaaaccaaat gaaattggaa tacactctat aagaggtggc
    26161 aatataatca gcgaccatga tgttgtattt gcaggtgata atgaagttct tacattaagc
    26221 catcattctc aatctgatga agtatttgca aacggctcta tagttgcagc taaattttta
    26281 attggtaagc aatctgggct ttataaaatg tcagatatat taggataagt tttgtacata
    26341 ataaatctca atctatttag gtattcatat tcatcaattt gaatatctaa ttgattgaga
    26401 tttctattct aaaagtttat aaattttatg cttacctttt aaatataatc atttttctta
    26461 aatattatct tatttattgg tatatgaaat catattgttt attattttat agtcttttca
    26521 tcatataaac ctattaaata ttatcctatt tattggtata tgaaatcata ttgtttatta
    26581 ttttatagtc ttttcatcat ataaacctat taaatattat tctatttatt ggtatatgaa
    26641 atcatattgt ttattatttt ataatctttt catcatataa actattgtat tctataaact
    26701 gatacatatc cagctgcaga agtacctgtc gtgcctgaac caaatgtaaa acttaaagta
    26761 acagctgctg ctctagcatc aactaaaaag cttaaactta cacttacatc tgatgaagta
    26821 agtgtattta ctgcttttgc aataaagtta ttttgagtta ctccattaaa gttagtagta
    26881 attgacataa atcctgcaag tgaaagtgtg gcacgtatac cgaaagatac attgtatatc
    26941 cctccaactg caagatttat agtactactt gttggaaaag ttatattagt tccatttata
    27001 aatgatgaat taaatgtcac taatgtatta ttcactaaac ttgaagagga cactgtaaat
    27061 tgtgcattgt ttgctgctaa cactcctgtt gctcctgtcg ctccggttgg acctgttgct
    27121 ccatttgctc cggttgctcc tgtcgcccct gttgctcctg tcgctccatt tgctccggtt
    27181 gggcctgttg gtcctatcgc tcctgctact ccatttgctc ccgttgctcc tactgctcct
    27241 gttgggcctg ttgctccatt tgcccctgtt gctcctgtcg ctcctgttgc tcctgctact
    27301 ccattttctc ccgttggacc tgttggacct attggacctg ctactccatt cgctcccgtt
    27361 gctcctactg ctcccgttgg gcctgttgct ccatttgccc ctgttgctcc tgtcgctcct
    27421 gttggaccta ctgctccatc tgctcccgtt gggcctgttg ctcctactgc tcccgttgga
    27481 cctattgccc cagctactcc tgttgctcct gtcgctcctg ttggacctac caaaccattt
    27541 gctccggttg ctccggttgg gcctgttggg cctaccaaac catttgctcc ggttgctccg
    27601 gttgggcctg ttgggcctgt tggacctacc aaaccatctg gacctgttgc tcctactgct
    27661 cctgttggac ctacctctcc atctgctcct gttgctcctg ttgctcctat tgggcctgtt
    27721 gccccagttg ttcctgttgc tccagtcgct cctatacttc ctgttggacc tgttgctcct
    27781 gtatttcctg ttgggcctgt agcacctgtt actccatttg ctccggttgc tcctgtattt
    27841 cctgttggtc ctgttattcc atttgctccg gttgctcctg ttgctccagt tagtccggtt
    27901 ggtcctgttg ctcctgttgc tcctgtattc cctgttggac ctgttattcc atttgctcca
    27961 gtcgctcctg tatttcctgt tggtcctgta gaacctgtta ttccatttgc tccggttgct
    28021 cctttgtttc ctgttggtcc tgttattcca tttgctccgg ttgctcctgt attccctgtc
    28081 ggacctgtta ttccattcgc tccagtcgct cctgtatttc ctgttggtcc tgtagaacct
    28141 gttgttccat ttggtccagt cgctcccata tttcctgttg gacccgttat tccattcgct
    28201 ccagttgctc ctgtatttcc tgttggtcct gttattccat ttgctccggt cgctccagtt
    28261 acacctctag gccccgtcgg tcctgttgga ccagtagcac ctgttggccc tgttggacct
    28321 acatcttgat ataaacttgg acctgaaata tcactcatat aattaatcct ccttttttaa
    28381 agttagagta atactctata aatattgagt attactctat ataattatat gaatcgacaa
    28441 tttcttatgt gataatgtat ctatttgctt atatcaacat atatgctttg attcttaaat
    28501 ttcattactt ttttgtaaaa tcttttacaa ttattagtac tcttttacaa ttattaatac
    28561 aattaaaaaa ctcatttccc atcaattatt taaaattttt ttattatgct ataaaaaata
    28621 gataattaaa ttgcataaaa ttaatttaat actactttaa ttacttttta aataaaaaaa
    28681 cctcaacacg attaataaaa atcatattaa ggcatccaca aaattatata attgcttatc
    28741 tatcttcatc agtaacattg aatatataag gaacatttct ataataatca ccaaaatcaa
    28801 acccatatcc aaccacaaac ttatcttcta tttcaaatcc acaataatct ggcactaaat
    28861 cgacttttct tctgcttggt ttatccaaaa gaacacagca ttttaaacta gcaggatttt
    28921 tagcttttag atgttttaac acaaaatcca tagtaagtgc agaatcagta atatcgtcta
    28981 caaccaaaac atcatatcct gctatatcag tagtcacatc tgaaactact ttaacctttc
    29041 cagtactttc ttcattgttt ccataactag atgtagtcat aaaattaact ctaagtggta
    29101 agtttatatt tctaactaag tctgcacaaa atataaagct accttttaat agagaaacta
    29161 ccataagatt tttatcttta taatcttttt ctataattgc tcctaattcc cttaatttat
    29221 ctttaatttt ttcttctgaa tacaatactt cccatttttt tgtttctata tccataaaaa
    29281 gacctccaat tgtaataatc cttcttaatt ttataagaga ttgctcactt tttcaatact
    29341 cttattcaat attagtcaat attattgagt ttttatgttc attcaacaat tattaacaat
    29401 gtatcttata ttttaagtag ttcatatatc cacactaaat tacatactat atattttaga
    29461 caaaaaaaga aaaaacttgc attaacaagt tttttctatt atacataaat ttatacataa
    29521 tcatatttta ttagagggta ctaattaaat ttatttatgc tatttttgtt atacctcata
    29581 atacaaaaca tattataaag tactagtcaa ctttattcat atcattcatt acatttaaga
    29641 ttatctcatc tgttatagac atacctttgt cacttactct acctaagttt tgtatacttt
    29701 gctcaacact tgaaccaact ataccattta gtggtggtac aaaacaatct tgcttagcta
    29761 ttatagcact ttgtatagct gcagatgctg cagaagataa ttttaatgca caaccagctt
    29821 tcgcaccgtc acatatcatt ccacttaagt cagctatcat attttctata gctccttcta
    29881 tttgcttttc agtaccattc ataagccaag agataccagc agttgctcca gtagaagcag
    29941 caacaccaca tccacataca gcagataatc ttcctgtata gttcttaata taaccagtta
    30001 ctagatgaga tatagctaaa gcttttgcta gtctttcatc agattgtggg aattttttat
    30061 tataagcaac gattggaaga attgctgtta atccatgatt tccacttcca tttgaactca
    30121 taacaggcat ttttacccct gccattcttg catctgaagc tccagcagtt aacatcattg
    30181 cataatttat aacatcatta cctaaaagac cttcttctat agacttttta attccatatc
    30241 ctacaccaat accagttttt tgttttaatc catattcagc catctcttca ttcattttta
    30301 ctccatctag taaaaattct atatctttaa aatcaattcc ttctacattt tttattaatt
    30361 cttgtatagt tactgtatcc attaaacttt cagctttttc tgccgcatca tttgaagctg
    30421 atacttttgg ttcattgtct aataatactt ctccatcttt ttctaagaac gtaaaattat
    30481 catgtttagt tcttattctc actttagcaa tatgattttt tccttttaac acaacttcta
    30541 taaatacttt ctctcttgta tcagcaggtg ttattgatag tggagtatta tccatataat
    30601 cctcagctat tttaacttcc tcttttgtta gagtttctaa cacacttaat ccattttcag
    30661 aatgtccacc tactatacct agagctgctg caatttttaa tcctaatctt tcagtacctg
    30721 gtatacctac gcacatacca tttttataaa tactaggact aactaacata cgattttcaa
    30781 ctatttcttc tcctaataat tcctttgcct ttgcacatgc tagagctaag gctactggtt
    30841 cagtacaccc tactgctggt ttaacttctg cctttaacat ttccactaat tcttttctta
    30901 tatctctcat tatatataaa cctcccaatt aagtttagat ttagtactca aataattaat
    30961 taaataattt cttatctata tttatagcaa aatacatgcc atatttttta tatcttttta
    31021 tatttttagt cttatattct ataaccattg gtatgactgc ctctatttat gtattttttt
    31081 tatttgtata gagtatttat attctttaaa atggagaaac atatgacaat ttcgcaaaaa
    31141 gacaaaaaaa agtctcaaaa tgagattcta tatcattttg agacggctca aatatattat
    31201 tttttaactt ataaaaacca ttttttaatt cataaaaaaa gattctgcaa aacacttata
    31261 gtataagttt aaagctttct ttaaagttat gaatttagaa aaatttaaat tttatatagc
    31321 tttttattat attactatga aattatattg tatttttcaa tttttctata taatgtagct
    31381 cttgatattc caagtgcttt agctgttaat tctttatctt ttttataatc tttgtactta
    31441 tttaaagctt ttattatctc aatcttctct agttcatcaa gtggaattat cctatcgctt
    31501 ttaacttcta actcttctac agaggtattc ttaatttttt gaggaattac attcattgta
    31561 attattgaag aagaagacat attcacacta tattcaataa tattctgcaa ctctcttata
    31621 ttccctggcc acttatattc catcatagca tcaacaaccc tgctatctat gtcaataaca
    31681 tttttatcta atttttttga atactcttta accatatgct tgattataag tgggatatct
    31741 tcttttctct ctcttaaact tggtaatttt ataggtataa catttaatct atagtataaa
    31801 tcttctctaa aagtgccatt ttgaaccatt ttttctaaat ctttatttgt agcagcaata
    31861 attcttacat ctattgaaac atttgattta cttccaattt tattaagttc cttttcttgt
    31921 aatactctca ataactttga ctgtaaatgc aaactcatat ctcctatttc atctaaaaat
    31981 atacttccct tatgagctac ttcaaacatt ccaagctttc cacctttttt agctccagta
    32041 aaagctccct cttcatatcc aaataattca ctttctaata aattgtctgg tatagcagca
    32101 cagtttacag ctataaaagg attatctact ctattgctat ggttgtgtat ggctcttgca
    32161 aatagctcct tacccgttcc actttcccca gtaataagta ctgttgatgt tgatttagaa
    32221 gctattaggg cttccttttt agaatttctt atagctacac tttctccaat tatgttttcc
    32281 aattttattc tataatcttt attcatctta ttgtagtttt ttatagcatc tcttttatct
    32341 ataaaatcaa taacatatcc ctttaactca ttttttaata ctattttatt gatgttgtat
    32401 attcccttcg aatcatatcc gtatctttta taaaaaaaac tacaggattt gtctttctta
    32461 aagttattta tagaaggttt ctttataaaa tctaattcga caaatatgtt tttgccagtt
    32521 atactatcct ttatattgaa aagctcttta aattttgaat tatatttatc aatttttcca
    32581 tccacatcaa tagacacaac agctttgtcc atattgttta acagtgtctc taattttttc
    32641 ttttctaatt caagctcata agttttaatt tgagctttta atttattaga tattaagtct
    32701 gccattttgc ctaaaaagtt tataagacca cttttattat tttctataat ctgtgcttgt
    32761 tcctttgtaa aggctataag gcctataaca ccatgagaga ctccatcaca tataatggga
    32821 caacacacct ctgcaaactc tacacaagcc ccacttttat agcattccct acatatatca
    32881 ctactctttg ggtcatcaat tagtattgta atctgctctg taagtgattt tttaaaagcc
    32941 gtatatttat ctactttctc acctattttg tctatatata ctcctgtacc tgcaatccta
    33001 aataactcct tatttactat agttacatct atattgagta ctgatactat agcttcagct
    33061 atattttgaa tatcagattt tatatattct aaacctatca taatcatcat tccttatttt
    33121 tctttaacat aagtatttgt cttatttctt taagaatcaa gtatatctta tttaaagtaa
    33181 attggattga catcaggatt acaaagagca taacaattat tattaactca taatttattt
    33241 ccatctaaac atctccttat aacaaaagac taggcttaac ctagtctttt atattatatc
    33301 catatttata ttttaactca tttatcttct ttttcccaca taaatatatt ttaatttatt
    33361 tgactcttta atcataccat caaagtttaa tcttgaaaat atattaaata cttcatgtgg
    33421 atagtgaatc tttgcaacag aaccacaaaa tttatgttta agatagttta acttaacttt
    33481 taaattcttt aaatttaaat cctctaatat aacctctcca ttgcccttta acactctttg
    33541 tatttcagat gaagcttttt cacaatctgt aataacatta aattggtcaa ctattattat
    33601 cttgttgaac tgattatcat caaatggtag aaactctaac ttacctttaa ctacattgca
    33661 attttcatta tacactagag aataacagac atcttcataa ttactattct ccagtatagt
    33721 tacagaagtt ccaagacctc tcaatctctt gcctaggttt cctatatgac caacaagcaa
    33781 tattttatca cctacattat actcaattgt atccaaaaaa cattctaatt catcagatgt
    33841 atatttagct ttaaccggct gtttatttat ctgacataat atgcttttca caatgcttca
    33901 cctcttcatt tcctcaatta taaattatgt acagattaca ttataataaa tggcgagcaa
    33961 ctattcaaga gatatttcat tagtattttt ttaaacaatc tttatacatt ttaatatgat
    34021 attataccaa atttattagg attttattca taaaaatcag tctatttttt atgtttattc
    34081 attgttattt tcatccaatt tttttatttt cgatatggat acttcatatg ccattttttt
    34141 gaccacatta ttttcatcta tttttttctc atattctcta ctttgaaccc taccccaaag
    34201 ctgtattcta tcaccaacct ttagattctt tgcaaattta gcatttctac cccaaactat
    34261 tgatggtata taatcagatt tattataagg tctgtttata gcaactaata aatcggttat
    34321 ttcccttcct aaaggtgtct tcctatacac aggttcctta catacatatc catctaaaaa
    34381 tatactatta gggtctttat tttcctcgtc tatgcttttt atttctctga caaacactgt
    34441 aagcactaat ctatttttat tatttaatgt tttgttatat gatcttaatt ggcctacgac
    34501 attgactaaa ttttgtttat caaggtcaat atcttggaga agtctttctg atacagtcat
    34561 tggaagtata tcaaaagaat cgcttagtct gtttattttt attttcatat tataaaattt
    34621 ttctccaaat atctcatggc taaattctag cttattgtct aactcccctc ttaagtttac
    34681 tacgttattc tcatctttaa cagctatcat attttatccc tccacaattg ttaattctta
    34741 tatttctggt atcagcttat ataataaata tatttataag ggatgtattt tattacaaat
    34801 ctttgattgc tgtttaaaag tttttattta gttataaatt tcatttaata tttcactttt
    34861 ataattataa aattattatc tttttgtacc acatatacta attaaactac ctatttc
//
LOCUS       DAMKMI010000026        34697 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.027, whole genome shotgun sequence.
ACCESSION   DAMKMI010000026 DAMKMI010000000
VERSION     DAMKMI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.027"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC429_001201"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC429_001201"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF3846716.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTDPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="PC429_001202"
     CDS             complement(2851..3216)
                     /locus_tag="PC429_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3846717.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="PC429_001203"
     CDS             3415..4263
                     /locus_tag="PC429_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846718.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="PC429_001204"
     CDS             complement(4700..5788)
                     /locus_tag="PC429_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846719.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="PC429_001205"
     CDS             5913..6641
                     /locus_tag="PC429_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF3846720.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="PC429_001206"
     CDS             complement(6843..7334)
                     /locus_tag="PC429_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846721.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="PC429_001207"
     CDS             complement(7345..7788)
                     /locus_tag="PC429_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846722.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="PC429_001208"
     CDS             complement(7778..8617)
                     /locus_tag="PC429_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846723.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="PC429_001209"
     CDS             complement(8836..10221)
                     /locus_tag="PC429_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3846724.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="PC429_001210"
     CDS             complement(10394..11137)
                     /locus_tag="PC429_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF3846725.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="PC429_001211"
     CDS             complement(11157..12419)
                     /locus_tag="PC429_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3846726.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="PC429_001212"
     CDS             complement(12603..13601)
                     /locus_tag="PC429_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF3846727.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="PC429_001213"
     CDS             complement(13585..15096)
                     /locus_tag="PC429_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF3846728.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="PC429_001214"
     CDS             complement(15150..16112)
                     /locus_tag="PC429_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF3846729.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC429_001215"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC429_001215"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF3846730.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="PC429_001216"
     CDS             complement(17092..18105)
                     /locus_tag="PC429_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3846731.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="PC429_001217"
     CDS             complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="PC429_001217"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF3846732.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19516..19779)
                     /locus_tag="PC429_001218"
     CDS             complement(19516..19779)
                     /locus_tag="PC429_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846733.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19849..20922)
                     /locus_tag="PC429_001219"
     CDS             complement(19849..20922)
                     /locus_tag="PC429_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF3846734.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21181..21939)
                     /locus_tag="PC429_001220"
     CDS             complement(21181..21939)
                     /locus_tag="PC429_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846735.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(21936..22853)
                     /locus_tag="PC429_001221"
     CDS             complement(21936..22853)
                     /locus_tag="PC429_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846736.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22853..23053)
                     /locus_tag="PC429_001222"
     CDS             complement(22853..23053)
                     /locus_tag="PC429_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF3846737.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23058..24152)
                     /locus_tag="PC429_001223"
     CDS             complement(23058..24152)
                     /locus_tag="PC429_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3846738.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24662..24802)
                     /locus_tag="PC429_001224"
     CDS             complement(24662..24802)
                     /locus_tag="PC429_001224"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF3846739.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(25112..26419)
                     /locus_tag="PC429_001225"
     CDS             complement(25112..26419)
                     /locus_tag="PC429_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3846740.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIVHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26484..26963)
                     /locus_tag="PC429_001226"
     CDS             complement(26484..26963)
                     /locus_tag="PC429_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF3846741.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27010..27873)
                     /locus_tag="PC429_001227"
     CDS             complement(27010..27873)
                     /locus_tag="PC429_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3846742.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27873..28628)
                     /locus_tag="PC429_001228"
     CDS             complement(27873..28628)
                     /locus_tag="PC429_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3846743.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28655..29143)
                     /locus_tag="PC429_001229"
     CDS             complement(28655..29143)
                     /locus_tag="PC429_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846744.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29161..29562)
                     /locus_tag="PC429_001230"
     CDS             complement(29161..29562)
                     /locus_tag="PC429_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF3846745.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29565..30062)
                     /locus_tag="PC429_001231"
     CDS             complement(29565..30062)
                     /locus_tag="PC429_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846746.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30086..30490)
                     /locus_tag="PC429_001232"
     CDS             complement(30086..30490)
                     /locus_tag="PC429_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846747.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30720..33287)
                     /locus_tag="PC429_001233"
     CDS             complement(30720..33287)
                     /locus_tag="PC429_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3846748.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33453..34196
                     /locus_tag="PC429_001234"
     CDS             33453..34196
                     /locus_tag="PC429_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF3846749.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT        11241 a         6353 c         3558 g        13545 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttaaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggatctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcatgcc taacaatgtg cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggca tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctgtac aaggtatacc acaagctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtgttgg tgattcagaa
     4501 tagtatggta ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gcaaaaaaat
     6301 ataatgtaac cgattttcta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatgtttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagtttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaaaccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgatcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaat cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcatagttca aagaaaacct agagatttct ccatgatttc
     8521 tttttaagta tttacatagt ccacaatagt aagctttgta ataatcatat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttatgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttgttta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcctt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg tagctaacat ctccaatatc taatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg cacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg gtattcttat tggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcttct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta ccaagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttagta gtggatactt tttgagacaa
     9961 accatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccataact
    10021 agccacaaat gctgtatcca taccttctaa tccctctata ctaaaaccat gtttttttaa
    10081 acattctttt tgtaaatttg aagcatagta ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattatcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctacc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgttcag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccaaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaaccc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattac tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 ttttttccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gaccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacccgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatccacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattct cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaatat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcctcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcacc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattttgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatattt ttcaacatct tctttataag
    16201 gcataccaga ttgtgcacct agtttagtta cagacagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tgtttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattgcact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg tagccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgaa aaaaaaatta atatactttt ataattatct
    18301 aaaagtatat taattaattt aagcagtatt tattaaagtt caaatccttg ctctataatc
    18361 atttttttat cattttcaat cccacttcca acagttgtca aataatcacc aacaattgca
    18421 gaatttactc ccccattaaa tccaactttg tcatatccct ttaatgatag ccttccacca
    18481 gcatacctta tgtacacatt aggaattata aacctaaata ctgctgttgt ttttagtatt
    18541 tccattggtt ctaatacatc ataattttcc attggtgtat tcttaatagg atttaaaatg
    18601 tttattggaa atgattttac atttaattct cgtatttcaa aagccatttt tagtctctgt
    18661 tcttcactct cattcattcc aattataccg cctacacata catctagccc agctttttta
    18721 gcatctttta tagtttttat cctatcttta tatgtgtgtg ttgtacatat attatgaaaa
    18781 ttatctctac acgtctcaac attgtggtga tatgtagtca cacctgctga cttcaacatt
    18841 ttagcttgct cataatctat aatacctaaa gatgcacata attttaaatt agtattttcc
    18901 ctcaatcctt catagatatt taaaatatta ttaaactcct ttcctgacat acctttacca
    18961 cttgtaacca atgagaatct atgcactcct ttactctcca tctctttagc tctatttaag
    19021 atttcattgt aatctaataa tgaatattct tctacaccag ttttataata agaagactga
    19081 gcacaaaatt tacagtcttc actacatttg ccagatttag cattcattat cgtacataaa
    19141 tctgcttttc ttcccataaa atattctctt atttcatttg cagattttaa tagaatatca
    19201 aaatcgtttt tattatttgt atcaaggctt attaaattaa gagcttcttc ataacttatt
    19261 tctttttctc tcaatacttt attttttaat tttattatgt atttttccat atatcctcct
    19321 atttgtcaac ttaatatttt tatgtagttt acttattata tatagtattg catatataac
    19381 atttattggt caactttatt ttagacacaa gtttactata cacaaaaaaa tactgcttca
    19441 aaacaatatt agtatataga ttatacatat tattattaaa gcagtatttt ttattagaat
    19501 ataattctta ctggtttata aaactatcct tgtcttaact ccatccaagc tttccattgt
    19561 aataacttct aagccttcat attctctaaa tagccatttt aaatccttca tggcttgatt
    19621 aacctgactt tttgttagat tatttataaa atattctttt ctagtttcct catactcttt
    19681 gctagtcaaa aagtttctta gaaaattaaa cgcaaataca cttggaatat tgtttacatt
    19741 agccttcttg ctgttttcct tctcatagta aattttcata aatgcatact cctttgatac
    19801 ttttagattt ctcttacaat aattatatgc atttaagtgc tactattctt ataattttat
    19861 taattcatat tctttacttc ccatacccat ttcacatgca tagtctatta ttctagttcc
    19921 atttacatga ggatgttcat gtttgaaaac atcatgaccc atttccttac aaatcaaatc
    19981 atagcatgct tgttcaatag ctacagggtc tgttgatgct aatattccta tatcatgggc
    20041 aattggtcta cctgaccaag gtacacaatc acaaagtgga gttacattca taacaaaagt
    20101 tatataccct accttgtctt ttttatttga tactgctcca taagcgtatt ctgccatctt
    20161 ttcgacaaac acatctgagt cactttccca ttgtatagtt actgctctag ttggacagac
    20221 agttggacat tctccacatc cataacatac atcagaatct ataactgctt ttttatccac
    20281 tatacttata gcttttgtag gacaagaatt aacacacttt ccacaaccta cacacttctt
    20341 ttcctttact actggagtaa catcagaatg ttgcatttgt tttcctgctg cacttgcaca
    20401 tcccatagct aagtttttta atgctcctcc aaaacctgct gcttcatgac ctttaaaatg
    20461 actcattact atcattgcag aagaattata aatttctcca cctattttta cactttcaaa
    20521 atgttttttg tctattttta cattctcata atttctacta tataatccat ctgcaattat
    20581 tactggtgca tttacaactg cataagcaaa tccattttct atagcagttg taagatggtc
    20641 tacagaattt gttctactcc ctgtatacag tgtatttgta tctgttaaaa atggctttcc
    20701 ttcgcagtct ttaaccttat caaccacttg tctaactgcc acaggactca tatatgtagt
    20761 atttcccttt tctccaaagt gaagtttaat tgcgacttgg tcattttttt ctattaaatc
    20821 tttgaatcct gcttcatcaa ataatcttct tacattgttt ggtacgtttt tattttgcga
    20881 atttgagtat aaatcacaaa aatatacttt tgacttttcc atatctacac ccctttaaaa
    20941 tatttactta tactaatttt atcactttta aagtaatttt tagtatattt taacataatt
    21001 aaatattctt atatttattt gatacaaata atattatttg tattattttc tacacttaat
    21061 tctatatatt aaactacacc taaaaatcaa aaagacttga aacttaagtc caagtctttt
    21121 tgatttttag gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta
    21181 ttaaactggt tttttattaa ataaaataac agctgctagt aaacatacta tagaaataag
    21241 aactgttata ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc
    21301 tatatttttt gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat
    21361 taaactcaat aagagagttg ctaccaatac aagtaaaaca ctggcaaatg ctggtctaaa
    21421 tagtacacac ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa
    21481 tgctgaaaat ataatatgat taagatttgc agttggccaa aaatacattg tatagccata
    21541 agcaaataaa aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt
    21601 agacaatact atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt
    21661 agaatattca tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc
    21721 agagatattg ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt
    21781 taaagtcggt gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact
    21841 tataccaaat attagaaata aaataattaa actaaataat ttataatttc ttaaattctc
    21901 tttaaattct ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa
    21961 ttttcaagtg ataattctat tagttccatc tttactggac aaagattcat ttctgcgagc
    22021 gtagatataa ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata
    22081 ttagtttctt ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga
    22141 atttcatcat tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt
    22201 tctgaaagtg taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag
    22261 attctttcaa catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt
    22321 ttaagcataa tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca
    22381 tcacaaataa gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc
    22441 attccacgag aaaacccacc tattcgtctc ttttcatttc taagacccac aagtgataac
    22501 aactcttcac tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga
    22561 gaaagatatt caagtggtct catataattg taaaactctg gaacatcagg cagataacct
    22621 acatgacgat tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt
    22681 ccactatctg gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca
    22741 ttcttaccta caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt
    22801 atctttcgat taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt
    22861 cacctcttcc tattgtaaaa tacaaaattg gtcctattaa actaataaac agtactacaa
    22921 taatccatat tgccttattt ccaattttat aatttggatg tctcaacaca tgaacaagtg
    22981 cagtaattat aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca
    23041 tcaaattttc cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat
    23101 tctttgtttt cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct
    23161 gcttttacga ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca
    23221 agtttattaa aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa
    23281 tctacatatt ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt
    23341 tgtgcctgtg ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat
    23401 atatctgaaa ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt
    23461 tgcaaaatac ccactagatt ttggactaat gatataaggt tctgatatgc accaagttga
    23521 aatgtttctc ttgcttcatc catttgacca ttcatcattt gcccttgtgc aagaagtata
    23581 gattcattta tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga
    23641 tttaacaata tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca
    23701 ttacaagttt cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc
    23761 tttttttcat ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa
    23821 ggaaaacatg aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat
    23881 ggtttaaccg taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc
    23941 atttgaggtt cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc
    24001 ggtaaaaagg ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta
    24061 gaaacgccta tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa
    24121 atcagcgttt ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat
    24181 ccttaaacat gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca
    24241 cagagaaaca aataaacaag tatataaaat atatatttaa atttatcttt aatctccttt
    24301 gtaaaatatg taaactttgt aaataaaatc catattatat aaaactaata gttaatctat
    24361 ctaaatacag tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat
    24421 aaacacctta ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc
    24481 gattttccat ttaatccttt tataaagtta ttattcgtat ttattcctct aattataaca
    24541 ggcaactatc atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc
    24601 aatcatttag attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt
    24661 attattctcc attttcatcc caagtcataa atccccatcc actatctgga taaatatcga
    24721 tagacttttc tctaatcata taaccaactt tataacactc ttttgttatt ttattagata
    24781 gtatacatcc ttctccattt atctaattcc atttaattaa cttttctttt caaccttaac
    24841 ttttataaaa tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat
    24901 gagggtgtct caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa
    24961 tatattttca tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat
    25021 ttgatggtgt tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc
    25081 tgtaaatttg cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca
    25141 aagtatatat ctccatattc tttcgtatat gatttattgg tactctttca tctactccat
    25201 gaccaccagt tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac
    25261 ttgcatatgt gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat
    25321 aggcttctaa taatttttta agtactaaat tactctcatc cacataatgt aagtctttat
    25381 gaacaataac ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat
    25441 tgtttaaaac tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat
    25501 ttacttttaa tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa
    25561 tccccaatgc atctccataa taatcactac aaaggtattt tgaaactaat tctatatctt
    25621 ctctatgctg aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat
    25681 tttctccctt ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa
    25741 taattccatt aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc
    25801 cacttactat tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat
    25861 gaacccttcc tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac
    25921 ttggaatttc ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat
    25981 ttgtaccaaa aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt
    26041 tcaatacatg tataagcata gatacagcac ccttattatc tatgacacct cttccataaa
    26101 taatattgtc aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa
    26161 ctatatctaa atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc
    26221 caatataatt atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt
    26281 tatctaaaga ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt
    26341 gtgaatcaat ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta
    26401 cacttctgct tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttgaa
    26461 gaacaggctt ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca
    26521 tcatgagcac atttttgagc aaaatctaat ttaacacctt gtgcacagtc aactcttcca
    26581 taatccccat aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat
    26641 tctttccaag cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta
    26701 ttttctacta attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc
    26761 atttttcctg gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat
    26821 attgcttctt tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga
    26881 agatagaacc atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt
    26941 tctgtataca ttttttctga catttctttc tcctcctaaa attaaattct atattttaat
    27001 cactgtgaat tagaatattc caatgaatga ccctaccaat gctaatacaa ctaaaattac
    27061 taataactta gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac
    27121 accaagtggt aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt
    27181 accaccaata tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc
    27241 catcataaac attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga
    27301 tagaattttc ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc
    27361 ttcgcaaaat agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc
    27421 tgctccaaat gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt
    27481 atctccaatt ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc
    27541 tgggattttt ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa
    27601 tcctccccat atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa
    27661 atcttctttt ctcttatata acttttctaa agccggagtg aaacttgcac aaactgctaa
    27721 tgcttgcatt ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt
    27781 ttttgttaca tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc
    27841 atttttaact tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct
    27901 ttcataaagg taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat
    27961 atttcaaaat attgaaccat aaaaaatcct ataataaaca ttggtaaaaa tttattttta
    28021 cctatagtaa atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt
    28081 aatccattca ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca
    28141 aaaaagttac atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa
    28201 agtgttgcac atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta
    28261 tgaaccaata cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct
    28321 aaaggtaccg caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt
    28381 attgcaataa gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt
    28441 cctagataaa ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa
    28501 agtaatccaa ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac
    28561 cctgcaagac ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca
    28621 gattccattt ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt
    28681 catatgtcat agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat
    28741 taagttcatt aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt
    28801 tctttcctgc ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt
    28861 tataagcctt atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta
    28921 aagtgttgtt ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc
    28981 ctgatggtgc tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat
    29041 caactattat tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat
    29101 gaattaatct gtggtctact cttactaatg ttatttttgc cattatattc acctcataat
    29161 ttatattttt ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat
    29221 tctttctggt aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat
    29281 tgtctgtaag ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc
    29341 gacacttgat gtagatcctc catataaatc tgatattata attttcacat cttctttatc
    29401 tactacttct tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa
    29461 aacttcatat tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc
    29521 ccaagttcca tggcacagta tgattacctt atatttattc attgctaaat aaactctcct
    29581 ttatagcgat taatattttg gtttaattct acttcttctt catcaggtac catttgaatt
    29641 gatacattaa tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg
    29701 tatacatttt gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt
    29761 tttttgatat taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat
    29821 acaaaaacgt tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt
    29881 aatatcgata aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca
    29941 tcattggttg tttcatcatc tactactact atctttttta cacctagatg cttaacccat
    30001 ttatatataa cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac
    30061 atttttaatc aaactccttt ttctattaca ttgagctaag aactttatta atattgttta
    30121 taccagtttg tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc
    30181 tattaattaa agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc
    30241 cacctttttt aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta
    30301 atactaaaac tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta
    30361 agtcttcaat attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg
    30421 caataagttc acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta
    30481 ttcctatcac caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc
    30541 caacttatgt gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttact
    30601 caattttttt taaaaactta ttatttctat atgtatcaaa agattttttc aacctgtatc
    30661 aaatctgtat caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat
    30721 caacttttga atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat
    30781 tccatatctg aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata
    30841 actaaatata attctttatt tctactaatt ctttcttcat atgatttata aactaaagac
    30901 tcttttatta ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt
    30961 tcatcttgaa ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata
    31021 aaataagcct tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat
    31081 ctatcaaaat atctaccacc attatctttt tcagcatatg taacttccat atctccataa
    31141 tgtttcttta tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt
    31201 agagcctttc tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact
    31261 tcttttcctg tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt
    31321 aaaattaaaa ttccttttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa
    31381 atatcctgaa ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt
    31441 aaagtattgg ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat
    31501 attgctatac tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa
    31561 attgcttcat cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct
    31621 tttgctatgt tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt
    31681 actatatttc caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt
    31741 attaatttat ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt
    31801 acagtagttg caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta
    31861 tatataaaag tattcattaa attataggaa tacttctgta tgtctccatc tataacactt
    31921 ctaatttgtg actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt
    31981 ccataaaaat ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc
    32041 ttttcttctg gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac
    32101 tcctcttttc tattatttcc atataatgat tctctaatat tatttggtag aagagagagc
    32161 cttacatata cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt
    32221 aattgtatat cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc
    32281 ttaataactt cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa
    32341 aaataacata taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt
    32401 atagttacag gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta
    32461 gtggctgcaa ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta
    32521 tatatccctt tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga
    32581 acttcatcta aaaacaatac tccattatta gcttcatgta caataccata cttatcttta
    32641 tcagctccag taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga
    32701 ttatcagaat aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt
    32761 atagttaaac tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt
    32821 cctattatta atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct
    32881 atttgggacc ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt
    32941 atactctcta aaatatactc atctttgata gaatcatttt cactatattc gtttatagag
    33001 ttaatattat caaatatccc tttatttagt ttctttccta aaatactctc aatactttct
    33061 ttatataagt actgaacagg cttacccttt atctttataa taatactttc ttttattaat
    33121 tgatttaatt ccttactagc attacttcta tttataccta cttcagtagc tatatattct
    33181 gcatcaaatc caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc
    33241 aagcaaatta aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc
    33301 tataagcagt aatactgtta aacatttatt attaagctta attctacaat attttaatat
    33361 ttttgtctaa ttatgtaact attgattttc tttcctattc gttttataat ataaattacg
    33421 ttaccctcat cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc
    33481 actctatagt cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag
    33541 aaaatggaat taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc
    33601 acagatggta ctttcacaac attcaaaata tcccaagaat cataaataac attataattt
    33661 taagaggctg tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg
    33721 taattcctcg cttagattat cttattttgt catttcacga agctgtgttt tctccaaata
    33781 cattagagaa taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa
    33841 tattaggaca ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag
    33901 ctatagaaaa gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa
    33961 atggtagctc ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa
    34021 tcatactgac ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa
    34081 atatcttaaa ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta
    34141 ttaatcattt tcaccaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa
    34201 taatacatgc ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata
    34261 aagaaattat aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga
    34321 ttgatatggt ctatgatagc aatagaaatt ataacatttt taataaactt agttttatat
    34381 accttatagt ataaatctaa aaaagcatca tctaaaaagg agctgtcaca tgagcaaatt
    34441 aaatttgttc tatgatagct ctttttttac ttattttaat taggtatatt tttaattatt
    34501 ttatataaag cttatgttct aagtaaaatt atgcataaca aacaaaggta taaaaatacc
    34561 attttttcag ataatattta attataaata ataaatttag aatacttctt ttaaatgatg
    34621 agagtctaga aatttttatg tgtattcttt aaataaaaat ttccagactc tagccccttt
    34681 ttgaatattt taaaatt
//
LOCUS       DAMKMI010000027        33934 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMKMI010000027 DAMKMI010000000
VERSION     DAMKMI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33934
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.028"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(383..1450)
                     /gene="rsgA"
                     /locus_tag="PC429_001235"
     CDS             complement(383..1450)
                     /gene="rsgA"
                     /locus_tag="PC429_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF3846750.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            complement(1447..1848)
                     /locus_tag="PC429_001236"
     CDS             complement(1447..1848)
                     /locus_tag="PC429_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF3846751.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     regulatory      2645..2741
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2795..2971
                     /locus_tag="PC429_001237"
     CDS             2795..2971
                     /locus_tag="PC429_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846752.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3381..4019
                     /locus_tag="PC429_001238"
     CDS             3381..4019
                     /locus_tag="PC429_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846753.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(4508..6268)
                     /locus_tag="PC429_001239"
     CDS             complement(4508..6268)
                     /locus_tag="PC429_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3846754.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(6331..6474)
                     /locus_tag="PC429_001240"
     CDS             complement(6331..6474)
                     /locus_tag="PC429_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3846755.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            complement(6793..7032)
                     /locus_tag="PC429_001241"
     CDS             complement(6793..7032)
                     /locus_tag="PC429_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3846756.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            complement(7037..7453)
                     /locus_tag="PC429_001242"
     CDS             complement(7037..7453)
                     /locus_tag="PC429_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3846757.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            complement(7450..7749)
                     /locus_tag="PC429_001243"
     CDS             complement(7450..7749)
                     /locus_tag="PC429_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3846758.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            complement(8800..8967)
                     /locus_tag="PC429_001244"
     CDS             complement(8800..8967)
                     /locus_tag="PC429_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846759.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            complement(8980..11745)
                     /gene="mgtA"
                     /locus_tag="PC429_001245"
     CDS             complement(8980..11745)
                     /gene="mgtA"
                     /locus_tag="PC429_001245"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF3846760.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            complement(11911..12222)
                     /locus_tag="PC429_001246"
     CDS             complement(11911..12222)
                     /locus_tag="PC429_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF3846761.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            complement(12469..13146)
                     /locus_tag="PC429_001247"
     CDS             complement(12469..13146)
                     /locus_tag="PC429_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF3846762.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            complement(13284..13787)
                     /locus_tag="PC429_001248"
     CDS             complement(13284..13787)
                     /locus_tag="PC429_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3846763.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            complement(13803..16466)
                     /gene="mgtA"
                     /locus_tag="PC429_001249"
     CDS             complement(13803..16466)
                     /gene="mgtA"
                     /locus_tag="PC429_001249"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF3846764.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            complement(16559..16768)
                     /locus_tag="PC429_001250"
     CDS             complement(16559..16768)
                     /locus_tag="PC429_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846765.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            16718..17053
                     /locus_tag="PC429_001251"
     CDS             16718..17053
                     /locus_tag="PC429_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846766.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            complement(17252..18154)
                     /locus_tag="PC429_001252"
     CDS             complement(17252..18154)
                     /locus_tag="PC429_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3846767.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(18171..19178)
                     /locus_tag="PC429_001253"
     CDS             complement(18171..19178)
                     /locus_tag="PC429_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF3846768.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQTMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVTHVGIYVGNNQMYHAGDPIGYTDLSSSYWQQHLIGAGRVK
                     Q"
     gene            complement(19175..21379)
                     /locus_tag="PC429_001254"
     CDS             complement(19175..21379)
                     /locus_tag="PC429_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3846769.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(21379..23829)
                     /locus_tag="PC429_001255"
     CDS             complement(21379..23829)
                     /locus_tag="PC429_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3846770.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYEMIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(23807..24205)
                     /locus_tag="PC429_001256"
     CDS             complement(23807..24205)
                     /locus_tag="PC429_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3846771.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(24323..24826)
                     /locus_tag="PC429_001257"
     CDS             complement(24323..24826)
                     /locus_tag="PC429_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF3846772.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(24844..25347)
                     /locus_tag="PC429_001258"
     CDS             complement(24844..25347)
                     /locus_tag="PC429_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF3846773.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            complement(25266..25562)
                     /locus_tag="PC429_001259"
     CDS             complement(25266..25562)
                     /locus_tag="PC429_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3846774.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            complement(25661..26455)
                     /locus_tag="PC429_001260"
     CDS             complement(25661..26455)
                     /locus_tag="PC429_001260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846775.1"
                     /translation="MGTINYFLKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYE
                     KYLLELVNKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKE
                     LTGQIQKFCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFD
                     IQTYVKLLTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAE
                     YDTFLAYIYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            complement(26494..26715)
                     /locus_tag="PC429_001261"
     CDS             complement(26494..26715)
                     /locus_tag="PC429_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846776.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(26716..26850)
                     /locus_tag="PC429_001262"
     CDS             complement(26716..26850)
                     /locus_tag="PC429_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF3846777.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSISKEADYEMKQLEESEDN
                     "
     gene            complement(26863..28059)
                     /locus_tag="PC429_001263"
     CDS             complement(26863..28059)
                     /locus_tag="PC429_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF3846778.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(28244..29638)
                     /locus_tag="PC429_001264"
     CDS             complement(28244..29638)
                     /locus_tag="PC429_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3846779.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            complement(29732..30169)
                     /locus_tag="PC429_001265"
     CDS             complement(29732..30169)
                     /locus_tag="PC429_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846780.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(30271..30654)
                     /locus_tag="PC429_001266"
     CDS             complement(30271..30654)
                     /locus_tag="PC429_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF3846781.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(30670..30996)
                     /locus_tag="PC429_001267"
     CDS             complement(30670..30996)
                     /locus_tag="PC429_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF3846782.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(31122..>33934)
                     /locus_tag="PC429_001268"
     CDS             complement(31122..>33934)
                     /locus_tag="PC429_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF3846783.1"
                     /translation="FKNGYKTRADASTRMSADQISDVALSLEYVKQYGEAHKELNYKQ
                     VYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDEVFAGAKAFVKENKGRYECGG
                     YIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNGNYSIAGATYGVFSDKDCTKQ
                     LATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTTVYSLKVEAGKTATLNVSDTP
                     KVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYAGFYNKDNLPAEATRTWVTKT
                     IAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAILPLGTLSVEETKAPNGYLLDGAY
                     MQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDKVIRGGVKIQKRDLETSDTKP
                     QGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIRTDIEGVASTSADLLPYGKFR
                     IVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSIYNQIKRGDIEGVKIGAGTHK
                     RLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHKHNTNAGKTSEDGVWFGTSEA
                     DDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRNNLVIDLGTLTDEYEKEISIH
                     TTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLKGWQMLKEENAELIIDGKRVE
                     NDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYDLSNPDEPVKVAEHKDIEDDG
                     QTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVTLEGLEVGTQYKLVGWQMLKE
                     ENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSLDGKQLVTFEELYDLSNPDEP
                     KKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPNRPSDSPKTGDSTNVMAFLVM
                     LLTSAGGLAGTYLYKRRTMKKS"
BASE COUNT         9938 a         7391 c         5785 g        10820 t
ORIGIN      
        1 caataatttt tataaatata ttatttgtat tttataattc atatattttt actttataaa
       61 ttagcaatat tgcagttttg ctcttaagaa acatctctat catacataag catatactct
      121 attatgtatt agtataaata tgccttttct tatcttacat aagacctctt tataaaggag
      181 cctatcgtaa caaatatgta tacattatca catgtatata tcatcatata aaaaatcaaa
      241 ctttattaaa aaaattaata aattgcttta aataatacca caaaccaaat ttgccatcaa
      301 aatcacatta aagtaacaaa ttgatggcaa atttaatata ttaatagtat atagactcat
      361 agaatgaaca aaaaaactac acttatcttc taatcttctt atttattttt gagatatttt
      421 taaatttctt ttcttttttt gctaagtatt cgcttttatt gtctgcaaat actgcttcag
      481 ttttaagcct acgataggat tcccatcttt taggcgaaag ttcttgatga gcaatagctt
      541 cccagatagc acagcctggt tctgaggtgt gagtacaatt acgaaaatga cattttccga
      601 aataagcttc tatatctgca aaacttttat ctaaaccttc tgaaacatcc cacattccca
      661 actctctcat acctggtgtg tcaataacaa taccaccacc tgaaattaaa aatagctctc
      721 gtctagtcgt ggcatgtctt cctttatcat catctatgcg aatttccttt gtatttaaaa
      781 attctccacc catcagacaa ttaattaatg aagattttcc aactcctgaa gaaccaagta
      841 aggcaacagt acagcctggt ttaatatact tagaaatctg tttataacca tctggctcta
      901 tagatgaggt aacaactaca tctacaccca aggctataga attaactact tgtaatttac
      961 gatgcacatt gtcacataag tctgatttag taagtactac tactgggata gctccacttt
     1021 cccatgttaa agacagatac ctctccaatc tatgaatgtt aaaatcctta ttcagtgaca
     1081 tgcacaaaaa aacaatgtca atattagctg caaccacctg ctcctcattt acctttccag
     1141 cagccttacg cacaaacaag ctttttctat gaagcacatg atgaataata gcattaccat
     1201 ttttattatt gcaacgatcc aacataacga aatcaccaac tgtgggaaaa tcagattgta
     1261 tagtagcctc aaaacgcagc tttcctgaaa cctctgcaaa cagtacacca tttacacata
     1321 cagcttgata aaagttcttt tcctgcgaga taatccgagc aaggtataaa tcatgatatt
     1381 ccatactttg agtaataaaa tttctattta gaccatagtc ttgtaaattg atttgtttca
     1441 taccattcat tctgtttcct ccttttccaa cttttcaatg attctttcta gtgggaaagg
     1501 gggctgtgcc agtggtttta atgcaattga cattaatttc actgaattat catcagctgc
     1561 aatacgatta gaactaagat gtccacagcg tgtacaacga tgaataattg cccactcacc
     1621 attttttctc acccacactc caataggttc catattagca tcacaatctg cagcacggtc
     1681 accaggaatg atatctacat gaagactatg taaacaatgt ggacaatgat ttctatgttg
     1741 tgtaccagct ccttcaggaa ctacaaggca accacaattt ttgcaaataa agttatccat
     1801 acaggaattt tcctgataat aagctcgttt tctattttta taattcattt ttatttcctc
     1861 cgtatcgtgt attttctaca aaaaaagtcc acaggtattt atatctgcct ttttatgtgg
     1921 tatacatatt tacctacaca aaaacagaaa acccctgtgg actagaaaat ttagtatctt
     1981 ctatttcaac ataattatat tcaaaataat actgtgtaaa attaataaaa gtgtagcaga
     2041 ttaaagccca ctacaccaca atttaacaaa gtaatatgta atattattat ttgttgtcta
     2101 atgttagtta tctataaagg cagagtaaga cacaacaatc aaatgtatca aaaatacact
     2161 taaaaattct gattaaacag aacccatatg cgtctccaac tatttatttg ttggttacca
     2221 ttatatataa ctctgacatc aggcaagaca ctccttcact aataattatt attgaaattt
     2281 tatcataaaa tataagtatg ttcaagtata atttaatata ttcatatatt tatgtttgtc
     2341 tcattttctt attctataaa atctagtggt attttgaaat ttaatgttca cctagtatta
     2401 ataataaaaa ttaactgcta ttatttgttt tctcatttta tgcctgttgc tcaatttatc
     2461 tcacttaaat taataacaac tgtcttttca tacaaaacct acaatcaagt ttttccatat
     2521 attctacatg catagtctat tttttttgat ataaaataac gttcatattt gtaaatttat
     2581 ttaattttca aataagtata ttgaataata agcaattcta ttgtattatt gtaattgtag
     2641 aataaaacta aatcggcaaa actagagaaa tttagtgacg caaagctata gggactaacg
     2701 cttataaaat aaactatgag ttatgtcagc cagttgccaa aaagagatat tctttttgtt
     2761 tttatgaaag ttttttaggg ggataataat atatatgttt agagataaaa tggatagatg
     2821 tacacatatg ttgactgctt atattggtag ttcatatgat tattgtgatt ttatagatac
     2881 acaactagat gattttatac tagagtacgg agaaaatgtg gtagaatctt gtttgcatca
     2941 agtgatggta ttagtaagta agtataatta aatagatttg gttttctaga gaaccctttt
     3001 tagttttatc tactttaaat agccttatgt tagaaagcta attaagatag atttctagta
     3061 ttcataattt tattttttat ttaaaataca tataataaag atgtaaattt gtatcaaata
     3121 ataaatatga gaacttaaat tctcttatct atgtgaaaat aaacatctct taaaatgaaa
     3181 ttctgtaaat ataaatgatt ttttagtttg aaggattaga ttaacaaaat tataaatttg
     3241 caaataagct atattaaaaa atataataat tttttatata acttattctt ttataacttt
     3301 taattttaaa agaggagaat gtatataaga gtattcaatg tactagaaaa aatcaaaaga
     3361 aatattagaa gaaatggata ttgagtaatc aaagaaaaga ttttgatatt acaaatgata
     3421 tgtttcaaga aagcattcct atcacaacaa aaattattct tgaagacatg ccttcaaatg
     3481 atgaattaaa tcatgttttt tcaaaaggtt gtgaacgaaa aatgaaaaag agaaaaattg
     3541 tattaatcac actactatta ataggtgttt tgttattagg aagtatccta tataatcttt
     3601 ttttaggaaa aacagctaat atttcaatgc taaaggaatc ttggaatttt gatataccaa
     3661 tacctaacaa agaaattgaa gtatttgata ctcaagatag tattaatgga gatggacaaa
     3721 gttattttat tcaaggattt tctgaaaaaa atttcaaaaa agtttttaat ttaaaaggtg
     3781 gtatagttgt aagcaaagat aatattaatg aaatagaaaa atatattgat aaatttaaaa
     3841 gagattctgt taacattaat aaaagtaata aaaataaaat tgaagaagat tttaaaaaat
     3901 ataagttaga agttaaaaaa gatgataagt atatatataa gagaaattat gagaactacg
     3961 tagttttaat tataaaaaaa gatgaacaaa aattatacag tttaatatgg aatcagtagg
     4021 tacatatgta ttattaagaa ttataaataa taaaaataaa ttttagtcaa tatacaaaaa
     4081 ccttttaaat tgtctggaaa tcacctcttc atttgttata aaaacataca acaaatgaag
     4141 aggtgatttt atggttttaa aaaaaataat tataagtgga tatgtgcttt cacatgttta
     4201 tcaacatttg taatgcctat atttgctaat gaattataaa aatattaaat aatattcaac
     4261 tatagattat gtaaattact ctatgaaaaa aatgagttgt ataaaatctg gctactgtag
     4321 attgactatt tcttttaaaa ttacaattat aataagtttt atagaattaa aaataaaact
     4381 ttagagattt ttgataacat tgtagactaa atttgccacc gaaatcgctt tacagtggca
     4441 aatcggtggc aaatacaata tattattagt atataatcta tataaacatt actattgttt
     4501 gattatctta gctacagttt ccacatgtgt cgttactact atcggaacac attttatcaa
     4561 cctcgttgct gtcatgtgaa cacaattcat tagtcctatg ccgtcctctt gcatttttcc
     4621 tttgtgtttt aaatttagca ccatctacgc tattcttcaa gcctgtttta taaacaaagg
     4681 tcaactgtgc agggtcaaca tttccgtttt catcaagtcc acctacaatt actttttcaa
     4741 caatgctttc aaacacatag cggtcaaatt tatcaagaac ctcattctgt tccagagttt
     4801 tcttaaactc tttcaaacgt ttttttatgt ctttctcatt atcagaagtt tcttgtaact
     4861 tttgtctttc ttcaacaagc tgttcttgtt tacttactaa atcagcatat ttactttcgt
     4921 aggtttcctt atcaataatt tcttccagac gcatatccac aagtttactt tttttcttct
     4981 ctaatgcgtc tatctccttt tccgcttttg caagttgttt tgacaccaca ctactactaa
     5041 gagtatcgtc cattctctgc aaaaattctt ccagaacatc tttattatca tcacataata
     5101 aacgataact ttctacaaat gcttcttcaa tagctgtttc tggaatacct ttgctatgtt
     5161 tacaaaattt ctttcctttc ttcgtagccg ttacacattg ccatataacc ttgctgtatt
     5221 ttgagccact gtgccaatta cgtcttgtta aagtaccacc acaaaagcca cattctagca
     5281 tacagctaaa tgcgtatttc ctgctatact tctcacgctt accgctgcct accgttgttc
     5341 ggttcttatt tctccgatta agaatttcct gtgctttttc aaaaacttct tcactaataa
     5401 tcggctcatg gtgatctttc atataaaatt tatcttcttc acccatgttt tcaaggcgac
     5461 gtttggaaat cggatcaaca gtaaaagttt tgcctaagag tatatcaccc ttgtatttct
     5521 cattcttaat aattccaata accgtagatg gtgcccatgt ggaactccca tacttcgttt
     5581 tataacctag actttcaagc tcctgtccaa taacagaacc accagcacca tcaacataac
     5641 ggttgaaaat atatctgaca atctctgctt cttcttgatt tacagttatt gtcttatcct
     5701 ctgggtgata atcatatcca atacaacctt gaaaacctac cagctcgccg cgcttcattt
     5761 tcattttcaa cccttttttt acattggctg aaatgttctc gacttcctgc tgtgcaacag
     5821 aactaagaac aacaagcaat aactctccat ccattgtaag tgtattgatt ttttcatctt
     5881 caaaataaac ggcgatattt ctctctttta gcatacgaac atatttcaat gtgtctaaag
     5941 tatttctggc aaatcttgat atggattttg taaataccat gtcaatttca ccattcatac
     6001 agtcatttat catacgctga aaatcttctc gttttgttac ttgtgtcccc gtaattgctt
     6061 catcagcata aatgtcagct aacacccatt ccttgttttt cttaatcatg tcagtataat
     6121 actgaacttg tgatttataa ctgtttaact gatcttcgct atctgtactt acacggcaat
     6181 atgcagctac acgcaaacgc tcaatggctt ttccacgggc ttgtcttgcc aaaacatttt
     6241 ttgcttttat tacttcaact tcctgcatag tacacctcct tattttgtgt tcctttcata
     6301 ttgttattgt actactttaa aaaacacaat tcaagcagtt atgtcagaaa gcaccttata
     6361 gtctttcatc agcctttgtt ttacaagagt gtattctttt tcagaaatca gtttacgggc
     6421 aagcagttgt tttaacatag caatttgcat actataccta attatattat tcattaaaac
     6481 cctccttgct tttttattta tggaagtaat cagacggctt cggcagctcc actggacttt
     6541 caccattccc attctgatgg gtgaaccgtg tcatacgggt gtatcattat tcagatatac
     6601 gagtcatggc agcaaccttt ccaaagatcg cttgcggttc actggcataa tcgctcgctg
     6661 ctttccccat ttcagtggtc gtggcgttcc agtccgctgg ctcgtcgtat atcaaacgta
     6721 tctgattact gtattcactt gtcaaagaac ggaaaagagt taaaatgagg tggtagatac
     6781 tctctgttat atttacttca ctttgacttc aaaccctaag attactttaa tcaacgctgc
     6841 ccgtatcctg cctttcagtt ccatatcaag cacgatatac atattcccgt gttcgtcata
     6901 gaacgggcgt aggctggctt ttgatatgta agcgtcataa tggttcaaaa tcttttcaat
     6961 tgccacctcg ttaccgtcag ctgcagaact gatggtcgag aatgatggac acttttttgt
     7021 agacttcatc atgtttttat tcctcctcgt acatatcctt aataagcttt aatgtgtgca
     7081 gcctgtttct acatacggaa aatcgttcca tttccataac ctctgcaatt tcagcgtcgg
     7141 tcatttcaag aaagtaggac ataagcagaa tgttacgtcg gctttctgtt aattctttga
     7201 tagcttcaca taagcgttca tcatagatat ggacttctga accaaataca tcaaatgacg
     7261 taaactcaac gcaatattcg tccatggcac cgatctggtt taactccatt tcagataaat
     7321 cacagaacgg cgtttcacgt cttgcacgtc tgccaagctc tcggtcatag ttctttacag
     7381 ttcgtcctac aaccttgcga gctagacaat caaattgaag tctgatagcg ccctcgaaag
     7441 aagatggttt cataatctca ccccctttcc agcgaaagtt gctaaagcga aaagacttct
     7501 acctcttttc gcactaacac tcaccatgaa aggtgggatt tgatacccaa ccgcaaaaat
     7561 tttttgaaaa agttttatgc agtaaaaaag cacaagggca atacagacgt actgatcttg
     7621 tgctaaacaa attattcctg ttatatgctg taaaaaacca catataggag cattatgttc
     7681 cgtagaattt aaatgttttt ttaatgaact accgatggtc gaaagttgtt gaatatgtaa
     7741 gtgtttcata cggctacccc tagagccgat aaaacaagac catccagtaa tttgtccaac
     7801 gttaggcaaa aataaaacgg cggctatata accgtcgtct tattcaaaag ttcaaaaaga
     7861 actaaactaa accctctaaa gcagtatgta tttataatca gtcttgcaaa ataacaaagt
     7921 agaattgaaa tataaggtag caatccttgt gcatctaata aacttatagg ggttacagca
     7981 gaccacggaa taatattttg tatactttca gatcaatata gtccaacaaa accaattccc
     8041 gatatttagt aactaaaaag taaactagag tagaaaacat ttctttgcaa aacacaaata
     8101 ataagtaatg gactcattga caatagattg agaaatccag ttttttagac ttagatagat
     8161 tatctcactt gtaaatgatt taagttcttt gacttcatat atagcggtat aatctgtcaa
     8221 cgtgatcatt acaaaccata atgcctatat aacatacttt ccaagcaaaa actttattct
     8281 attagttggg gaatacaaaa tatttatcat tgtgcgttca acatattctt tttgtataaa
     8341 aatgtaatta caaaacacca ctcaatataa gatagataat aatttgaata ggaattatca
     8401 atctacgtaa ccattcatta cctgtcattg ttgttacaat agatttggga ttctgtttcc
     8461 attacataat ttttttatcc ttctttcaga tatctcctca ttttatcgaa gcttcttcta
     8521 cagacataca ttccataata aaatctacct ttgaatatta tttcacgatt tcgttataga
     8581 cgcgtaaaca aacaataaaa aacgaaattt cttttaagta ccataatttt tatatcgaaa
     8641 ttacaattat ccacttatat acagcattta taggtaattc aatgataaaa aaacacatac
     8701 tttactacta aattgccaca attgaatgcg ccttgatatg atagggagat tccacaataa
     8761 aaaactgtgc atctcccttg attcattcat actgatatct tatttataag actcttttgt
     8821 gtcttttttt ggtttcatgg accaaaatac tacattcatt acaataacaa tcagcaacac
     8881 aactgccata gccacccaaa agccgatatt aagaccaagc cattcggtag ttccaaaaag
     8941 ggtcatccaa agttctttcc agttcatcat tccacctcct taaagcagtt ctccgaaatg
     9001 acggacatag gcttttttta aacttgttgc caaagtcata tacagcagaa tacatggaat
     9061 cagataagca aagtatgctg caggcaatgc aacaaatcca agcatagttc caaacacagt
     9121 aaacggaata atggtcagaa ctataattcc agtcattgtt agcaaagtca ccggagctga
     9181 tgcacgactc tgaacaaaag gtagctttgg agtacgaatc atgtgaatga ctaatgtctg
     9241 gctccacata gattccacga accaccctgc ttggaacatc ccgatatact gtgcctgaag
     9301 cattgccagt tctgcaccgc tgaaatgagc aggcaaatca ttaaataata caccattcga
     9361 tacgaacaat ggacaaaata caaagtacat aaaaatatag gttgtaaaat caaagataga
     9421 actggttggt ccaatccaga tcataaaact tccaacactg gaagcgtccc atttacgagg
     9481 tttggtaata aactcctcat ccacattatc ccaaggaatt gcagtacagg ataaatcata
     9541 aattaagttt agaaatataa gatgaacact catcattggt aagaaaggta gcaaagcaga
     9601 tgcagcaagc actgagaaca tattgccgaa gttggatgaa gcagtcattt taatgtactt
     9661 gatcatgttg gcatatgtct ttcgtccctc aataataccc tgttccaaaa ccatcagatc
     9721 tttttccaat agaataatgt cagcagattc ttttgccacg tccacggcgg tatccaccga
     9781 aataccaata tcggcggctt tcatagctgc agcatcatta ataccgtcac ccatgaaacc
     9841 gaccgtatga ccattttcac ggagaacaga aaccacacgg gctttctgat caggggttag
     9901 ctttgcaaat acatctgtag actctgccac tcttgcaagc tctgcatcgc ccatttgttc
     9961 caaatcagaa ccgagaagca tattgcgaac ctctaatccc acctgcttgc agatagttcg
    10021 agtcactttg tcgttgtcac ctgtcaaaat tttggttata acaccatggt ctttcagagc
    10081 tttaatagca tccgcagtag attcttttgg aggatcaagg aatgccagat atccaatcag
    10141 taccatatca cactcatctt taacaccaaa tgcgccgatt ggggaaggat tactcttctg
    10201 tgcaatagct agtacgcgga atcccttatc gttaagattg tccactgtct ccagaattct
    10261 gtaacgaacg tcatcagtca gaggctgcac tcctccatta cactctgcaa acgcacaaat
    10321 agagagcatt tcttccacag caccttttgt taccatctgg gtcttaccct tattatcctg
    10381 taccactgta gtcagacgac gacgtgtgaa gtcaaaagga atctcatcta ctttcacata
    10441 gttttcagac aggtcaatga gacgcgaatc tgcggcttcc tcttcctccg tcttatggat
    10501 aattgccaaa tccatcaggt ttttatagcc agtctggaag taactattga ggtaagcgtg
    10561 acgcagtact cgcgtatcgt cctcgccatt tacattcaga tggtattcca gcacaacctt
    10621 atcctgggtc agcgtgccag tcttgtcagt acagagaata tcaatagcac cgaagttctg
    10681 aatggaattg aggtttttaa cgattgtctg cttcttgctc atggaaacag cgcctttcgc
    10741 aagacaggtt gttacaatca tcggcaacat ttctggtgtc aaaccaacag caatagaaac
    10801 gccgaataag aaggccgcca accaatcgcc cttggtgata ccattgacaa agaacaccag
    10861 cggaaccata atgagcataa aacgaatcaa tacccaagaa acggcattga cacccttggt
    10921 aaagcttgtt tcaaccgctt ctccagcaac tgcggacgcc atagagccaa acagagtatg
    10981 atcgccaaca caaacaacaa cagctgttgc gctaccagaa attacattac tccccataaa
    11041 agcaatgttt gtgtaatcag tcacgctttc tttctgagca cttacattgg gtgttttttc
    11101 aataggttca ctctcaccag taagacttgc ctgactgaca aacaaatcct tcgcatccaa
    11161 aatacgaaca tccgccggaa tcatatctcc agcagaaaga tgcacaatat ctcccaccac
    11221 caaatcatcc ataggaagtt cttttttttc atagtttcta cgagtaactg tacaagtggt
    11281 cgtaatcatg gctaacaatt tctctgctgc atttccgctt ctggattcct gtacaaaacg
    11341 aagcgtaccg gaaatgaata ccattgttaa aatgattaca acagtcaggg aatcaaagtc
    11401 ctctggcaca ctgccaaaca gagaaaaata cggaaaaatc atatctgtaa ttgtggatac
    11461 taaagccaga caaaataaaa tggcggtaaa tggattgata aatgcgccaa ccagtcgttt
    11521 tgccagagat ttcttttttt catgagtgac tttattggta ccatatttag cacgacttac
    11581 ggatacttca tctacttcta agccacgaag tgtggtatga agttttttta aaacttcttt
    11641 aataggattg gttgccgcaa attgaatgcg gcggttctgt tcgtcacgga tgactgcttt
    11701 ttctacagtc tgacgaatag ccatacgatt ttcttttttg ttcattggtc ttacctcctt
    11761 gaattttcat aggggcaagg aatattatat gagcgacacg gaatctccaa attctcatat
    11821 aatattctct gccttcgtgg actgacatca gagtccatgt ctttcacctc acaatcttaa
    11881 aaaaatatct atgtgaaacc tcaatgtgga ttattggttg cgatagcgga cacttttttc
    11941 gatataccag acatcaaagc cggcaacaat cagccatgcg attattgcaa atacaaacca
    12001 gtggtttgaa aatcccggca aaaatccttt cattaagaat ataatcccat ttaacgcaat
    12061 catttctcca acattacgac aaagcgggac aatattaatt ttatctttct cctcctgcgg
    12121 catatttttc cacagggaaa gatgacgata tccttttcca aaggccaacc aaaagcctgc
    12181 aagagtaaat aaaattccaa aaaagataca tgtaaaatcc atcgtttgtt cctcctttca
    12241 atgcttaaac ctgtttctta gttttgtcag cttcagaagt ttaatgaaga gcacaataat
    12301 agtaacagca agaataaacc agatcacaat actcacctga taccatatct taaagattac
    12361 cattctttgt tcaaagaaat tccaacccaa atagctacat aagattgtaa atacagggta
    12421 gaaagcaaaa agaattgctc gtttcaaatc ttcgatttct ctcctcactc acaacacctc
    12481 ccaaccagca ctaatcactt ccggaattac caatacctgc ccaacgattc cttctaaaat
    12541 attgtttttg gattttccga cagaacagta ttctgcgaca atctcaacct tgtcaccaac
    12601 cacatcaccg ctttccagat tgttcaaaaa gagcgtctta caggaattgc tgttaatcag
    12661 caataagcgt atatcctgct ctgccttttc ctgacatgta accgaaatac gatactgctt
    12721 ttcagattca tcactggaag taattgggtg aaactttctt gcaaagggac gtagaatcag
    12781 gttggaacca attaaaatag tagcggcggt gatcgccatt gcatacattc ccgtacttgc
    12841 aagaattcca atcgctgcag tacaccataa ggtggcagcg gtattcattc cacgtactgt
    12901 ccctctgtcc ttgaaaataa caccactgca taaaaaacca acaccggata taatactgct
    12961 tccgacacgg aatacctgat ccgaaccgta gagcatagga aacaaggtaa agaaacttgt
    13021 acccatgcag attaacacat tgatacgaat acctgcagga tgaccagtca attgccgttc
    13081 taatccaata agaaatccaa gtgttaagga gagggcaatt ctcaaaataa aatctgtgta
    13141 aaacatatga aacacctccc tttaactact aacagtatat aaaaaatcca aatacaatcc
    13201 tatggctaaa gtcttgcgat tttcttgcga tttggtaaga aaatagacgg actgaaaccg
    13261 aaaatggtct tagtccgtct ttgtcatatc tgatcattaa atttgtatcc aataccccag
    13321 atggtcaaaa tatatgtggg acaatccgga gcaggttcaa tcttttttcg caacttccgg
    13381 ataaaagcca taatgttgct gttatccaga atataatctt cctcccatac ggcacgataa
    13441 atctgttcct ttgtaaaaac ttcacctttg ttctggacaa gaaagtacaa aatatcaaat
    13501 tcctttggag tcaggttgac ttcagaacct tttatcagta cttttctgcc tcttggataa
    13561 atttccagat cccctgccag gattctatct gacagagtac ccactggcca atcgctttct
    13621 tctgatgctc ctaacaataa cgaagatacc tctccacgaa tcatctcctg aataccagaa
    13681 agtaaatcct tcgcacaatc actgtccggt ggatctctta gtagtttttc taatagccag
    13741 atttcaagag ccgaatccat cggaatataa ctaatattct ttttcatcat gaatgcctcc
    13801 tcttaaatca actcttgata ttttttctta taaaaatatt tggctactga attcagcagc
    13861 atatagagag ataccaccag cagtaaaaat acgaaatacc aaagaggtaa tttcgtcagt
    13921 ccaaacaacg acgcaccgcc gacaaatgta agggaagtaa aagcaataat ccccactaga
    13981 gttatagaaa tgaccggtgc agatgccctg ctctggaaaa aaggaatctt ggaagttcgc
    14041 agaaaatgta aaatcaaaac ctgtgtccac atagattcca aaaaccatcc agtctgaaac
    14101 agtgctacat actgaagttg cagggaagga tctgtaagct gcagataggt tgcccctcca
    14161 catagcatcg gacataggaa ataatacaaa aataggaagg tgacaatatc aaacagagaa
    14221 ctgatcggtc cgaaagacag cataaactgt ctcagtgttt tgccggacca gtctcttggg
    14281 gacagcatct cttcctcatc tacattgtcc caaggaagca caatacacaa cgtatcatac
    14341 agcagattca aaagcagaat ctgaattgaa gtcataggca ggaacggtaa aaaggcactg
    14401 gcacaaatga tggaaaaaat atttccaaag ttagagctag cggtgatttt aatatatttc
    14461 agcatattgg taaaagtttt tcgcccctcc attacacctt gctccaaaac attcaagtcc
    14521 ttttgcagca aaaccacatc ggcagcatct tttgctgcgt ccactgccgt atctacagag
    14581 attcccacat ttgcttcatt cagtgccgga atgtcgttga ctccatcacc aagaaatccc
    14641 acggaatgac cattatcctg catagcagag acaagacgca ccttatgtcc cggagtaagc
    14701 tctgcaaaaa catgagtatg ttccacagcc tttttaagtt cacagtctgt catttcatcc
    14761 agttgagtac cagttaagat acgctctgta taaataccca cccggcggca aacagacaca
    14821 gcaatcgctg cctggtcccc tgtcagaatc ttagggataa cttttaatct tttgagtgca
    14881 gttacagact cctttgctgt ctgctttggt gcatcaaaga aggatagata gccaactaga
    14941 gtcatgcccc attcgtcatc aggagttatt ttcttttgcg ctcccacatt ttttcgtgcc
    15001 accgcaatga ccttcattcc gtcctgtaac atttcaccta ctacagaaga tacactctgt
    15061 ctcgcatcct gccccatggg gagaagtttt ccacgatatt ctacatggtt gcagcgtgag
    15121 aatatatgtt caatatcacc tttcataata agctgactat tcccattgct atcctgtaca
    15181 agtgtactga caaacttacg agcatagtca aagggaattt catcagcttt ttggaatttt
    15241 gtcagaagat ttgcgtaatg ggcttcccgc cctggcatgg tattgcaggc aagaatggca
    15301 ttatcaatgg tattgcagac accagaatga tagcagctat tcagataagc aagatcaaga
    15361 acctctgcat tttcattccc aaaaatatcc atataatatt ccagcaggat gctttcattg
    15421 gtaagtgttc ctgttttatc catacaaagc acatccatgc taccaaagct ctgcatggca
    15481 ttaatatcct tgataatggt ctgctttttg ctcatagcaa gacttccttt ggcaaggcag
    15541 gctgtaataa ccataggaag catttccggc atcagaccta cagcaactga cagggcgaaa
    15601 gcaaaagatt ccatccattt tccatcagta atccccagta atataaatac aatgggaact
    15661 aatatcgcca taaaacgtat catcacccag gcgatggaat cggctccttt tttaaaggaa
    15721 tttttatcat cagaatccgg tttggtaaat cctccataca gggtatcttt gcctactgcc
    15781 agtacaatgc cttccccttt tccactgatg actgtggttg ccataaaagc gaggttttca
    15841 agctgagtca gtgtatcctg ctctccgtaa ctgagttttc ggcagctctt ttcaatgatg
    15901 gcgctctcgc cagtaatcgc agcctgagag ataaagagat ccgtaatctt cgtcaatctg
    15961 atatccgccg gaacacgatc acctgcagag aatagtacaa tatcccctac cactaattct
    16021 tcgccaggaa tctctattaa ctttccatca cgcctcactg tgacgctttc atgaactagg
    16081 cgatcaagct gagcagcagc gctctttgct cgcagctcct gaatcagacg aattacgcca
    16141 ctaattacga tcatggaaaa gatgataatg gcggtagtag catttctcgc aaaattggat
    16201 gctacgaaaa catccgtaac aagagaaaca atgcccagaa caaaaagaat cacatgaaac
    16261 gggttaataa atgcccgtct caaccgttgc atcgtcgtat catttttccg cccattgaag
    16321 ctgttcgctc cgtatttctc ccgcattgaa ccgatttgct caatggataa accatccgga
    16381 gatgagccaa tgtctcgata aatttctgat gttctacagt atgcgtattt tttgatacga
    16441 ctatcaaata gtgttgcttt tgacacgaaa catcccctcc tttataagta taagtaataa
    16501 cattttaaca cagtgttctg aaaatgctat atttatattc ttgctttttt cttgcaattt
    16561 actttgatat ggcatttttc agctcgatca aatccgtctc gttagggcga gataaatcct
    16621 tatcaaagtt ttcaaccatt ccttctaaat ttggagcacg gatggatacg gcaagcattt
    16681 tctcatagcg ctctcgcaca taaataggca tttccccttg acacatatga gtgctgacaa
    16741 acttcttctt ggttttcacc ttgaccatgt atcaaattta aatgaactac ttagaaaagt
    16801 tgctcataat aatatttgta ttatgagtga tagaatgaca aaagtggcag tatggaaacc
    16861 gagtgtaaaa acaatacaat tagtttgtat ggaatatctg aactacataa ttgaccactc
    16921 tgtaatttat gaaaaaatac aatgcgcaaa ttggaacggg acagaagaaa ccaatagtat
    16981 ttttaataat tccccttcac cattgattac tcttatctat tcttgcgatt tacaacaaaa
    17041 taaaacaaca taaattttaa atacgttgac tggcataatt catatgtata gtaagtctac
    17101 aattaagatg cgctttttct aatccaaata tattaagaaa aaagttttgc gaagccatta
    17161 atactttgtt taagatgtct ttcataaata taaagaataa tgctcttaac aaaagcgtgc
    17221 aaattcctaa agaaatgcac gctttcaata attatcctat aatcttccaa ttgctctcct
    17281 tatgtagcac taattcaaac tgcgatacct gtgttgcctt tgtctgatta tcaatgaatt
    17341 tcacggcaac cttgactttc acattgtccc catcaaccgt aaagataggg tttaccaatt
    17401 cagaatagag gtagtccctg ccgatagact caagcacatt tccagacaca tagtaagcaa
    17461 gctccttttc tgtggctgtt gggtatagct taaagaaagt ttccagaaat gcagtagcgt
    17521 catttatggt atccgcgtct acacttgcgt ctgcttctgg tgtctttggt tcatagtctg
    17581 atttctcgat tgctggtgca agggtagggt tctgaataat taccatattc ccgccagcgt
    17641 ccacatgaac ttttacggta taggttgcct taaccgctgt tgtctgttcc ccctctttta
    17701 tctgctgatc tacttcgtag gtagcggaaa aagtgtccgt tccagactgt tcaatgctcc
    17761 atacaatcac atctgtaatg gcagagctgg tcggtatatc cgttctaatg gtatccatat
    17821 tcaagtcctg caattccttt gtcagataac cgctgattgc ctgcgtcctt gcgtcaatcg
    17881 cttctttgct gttactccat gtgtaatagg actttgcaaa attcttcacg aaattttcta
    17941 tcccgttggt gtcctgcaag cgaagctcaa tcacttcttt ttcatgggtc gtgtgctggt
    18001 caatagcggt aaaattctta tatactccaa agctcacgct tgcaataagc accacccaca
    18061 atgcaatcac ggttttctta tgtgtgccta ccttgacagt acgtaccttt ttttctttga
    18121 cattttctgt ctgtttctta ttcttcttaa acatattttt caagtccttt ctattgtttt
    18181 actcgtcctg caccgattaa gtgctgttgc cagtaactac tacttaggtc tgtatatcct
    18241 atcgggtcgc ctgcatggta catctgatta tttcccacat agataccgac gtgggttaca
    18301 tacgaaccag cgttataggt ggaatggaaa aataccaaat cgccagcctt tgcctgcgag
    18361 agtggaagat gttgggtagc gtcatactgg gcttgtgccg ttctcggtaa ggagatacca
    18421 gcttttccat agcaccattg cgtcagtccc gaacagtcaa aggaagtggt tggagaagaa
    18481 ccaccaaaca catatttcca cccttgatat ttcaacgctt cattcattac cttttgtgcc
    18541 agctctcccg atacctgcgg aacagttaaa tactgattga ctaattccac atagaacata
    18601 tttccatagc cataccgcca gcctccgttc ttcgcaacgg ctatcgggtt ggtataggtt
    18661 actttctttc cacccgattt ctcacgggcg aagctctctg caagattaaa agtgtgtttc
    18721 tttccgtttc ctgccacata gcccacatag ccaccgccat agttatagga ctgtatcgct
    18781 acattcaaat cgtcgatatc ttgatttttg caggaagaaa gcagggacgc aaaatactta
    18841 cacccctgct tgattgagct ttccgtatct aaggaactag gcggtaaacc aagactttct
    18901 gaactctgca taacatcttc tgccgtaccg ccactttcta cttgaatgat agccagtagt
    18961 acattgacgt actcggagat accgttttct ctggcgtatt tttctaccat agtctgatgt
    19021 ttcaaaactt ctgcggataa gttcataccc gtaatgccag aagaaaagtt gctgttttcg
    19081 tcgtcgctgt ccgcactaat cagcacccca aagaaaagca ccagagaaaa gagaatagga
    19141 aacagacttc caatgatagc gatatgtttc agtttcattt tttcttctgt cctttcttcg
    19201 ttacaagatt acggattttc tctgtggtct gctggttctt ctggacagtc tgggtctgct
    19261 gaacctttgt cctacggtct ttggtgtaat tctggcgtgt ttcctttgac acggtaactt
    19321 tttctacatt ctgcctatat gtcgattggg tcgctttccc gtcaggagta ctagaagata
    19381 gcggacgttc tttgacaccg cttgtcttaa ctggttcgct tgctttcgta gtcggagttg
    19441 tggcagggcg tttgacttcc tgcatttttt ccgcacccgt ctttgaaacg gttgaagctg
    19501 tggctggtcg ctcatgggga cgggtagctc ccgttgtcgc tgatccgtca gcctttcgct
    19561 gtgcctgcct tgcttcttgt gccttttgaa gctccatacg cttgacagcg atacttttct
    19621 tatgctgttc ctgcttttcc aagcgtcccg tctgtctgga ctgctgttcc tgcaccatac
    19681 cacgcttgaa atcagacacg ctggactttg ccttttcctt tgcggaatac accgcataag
    19741 cggtctgtgt gggcatatcc ttgatattct ctttgacagc gtttgccttg tctttcactt
    19801 tatttttcgt atctaagaca gcacccacct ttgagcctgc acgctgtccc atactggaag
    19861 tggtattgcc ccgattttct ttagaagccg tattttttct ttcggctctc ttgccagcaa
    19921 cggcacttcc agctaccgca ccagccacac cgcccgaaat accgccagca cttacagccc
    19981 ttgcaatacg gtgttccata cgcctagccc gatgtcgcat gaacagatac ggtctgcgga
    20041 atatcctgcg tcccatgctt tgactgtcgc cagcgttgag agaaaacata ctcattaagt
    20101 cgcccagctt catatagata ccagcgaaac atactatctg caagaacgcc accatgaaaa
    20161 acggatagtc cgtggatatg ttataaaaca tactggaaat gctaaacgca accgttacaa
    20221 tgagcgttat tcctgcccgt gtcattatgg tattaaatac cctcacgatt gcctgctttg
    20281 ccatgctttc atagctcggt atcatggaaa gtagaaagct gataggtaaa aacatagcga
    20341 agataataaa aagtatctgg ctgaacaaca tcatacccgt aagcaagaat acaaatatcg
    20401 taataccaag attaaagaac aagaggaaga acaccatacc taaacggttt accacctgcg
    20461 gtatcgtcag attgttgttg tcgttgtcct cgatttctgt tttcacgact tcttctctgg
    20521 tctttccgtc ctcgtcctct ggacttgccg atacgagagc ttcgacacgg tctgtcccga
    20581 tttcctctgc gttgctgttt ccaaactgta agagtagcca cggctgttgt acttgaatag
    20641 aaaataggct gtcccgtatc aagtctacgc tgtccttgcc ctcgctgtca gagttgggga
    20701 gcatgatttt tgttcccaaa tcaagcgaag cggtactgat gtctgatgaa aattcattta
    20761 tcttcttgat gtagtcggga gcgtaggcaa taaacgaagc ggacagcacg aacaccacca
    20821 caaagttgat aacagcgtga agtgccttgc tggtttcccg ttttatcagt cccgtatagg
    20881 caacatacat tcccaccact aagataataa gaagcaggaa accaacatag aagcccgtag
    20941 aagaaaagcc gttctcggta acgcctgcaa gggtctgtat gctttttccg atactgtctg
    21001 ccatatcgtt aataaaatca agtttatagg cttcctgcac cacatagcca gttgcattgc
    21061 ttaaataaag gcttatcgtc cagacaaagt tggtaatgca gtaaagcccg tactgtaccg
    21121 attttccgat accgtccagc cagttccacg gaagccacga ccagctatta tcgacgaaaa
    21181 aatcaagctg gtagttggaa agcggatatt ttgaatatag attttctgcg tttatggtat
    21241 cgtccacaag ccccgtcgca tgagccaccg tccccagaag tgaaagcagg ataagggaaa
    21301 gtgccaccac gaacagagcc attttgagaa agtgaagtat cttccttttt gtgaacgcac
    21361 tctgtatcct ttctttcatc actccacctc atttctctgt acgggcggtc tagtatcaaa
    21421 ggcgtgtagt aattcttcaa agactgggtg tatctgcacc acaccgacac gcccgtataa
    21481 gtcctgcaat aaacattgtc cgttctccaa atcacgcaag cgtttctggt tgttttcgtc
    21541 gtccttgtcg ataccgaaaa attctaaggt ctgctttatc tcgttgatgt cagtagaacg
    21601 aaatgcaaac ttcaaaccga tattgttttt cagactttcc tttgacacgt cataggcaga
    21661 ctgggtaacg aaataaacgc ctgcctgcat agctcgtcca gcacgaacca gcttatttga
    21721 tagggtttcg ccttgtgcca catttaagaa cgcccacgct tcgtccaaat ctacaatctt
    21781 aaaaatgctt ctgtccgaat ggataaaatc aagggcaaag gtactaatca caatcagcat
    21841 agacaccgac aataattcaa tggtcgtgta ttcctcaaaa gtggtatctt tatctggcag
    21901 tacaaggtct gctacttgaa tgatattgag ctggttatca aggcttatag cattttctac
    21961 cgtcccgtct gaaaacagca ggtgtgcaaa gtcgtagtcc gtgaagctgt cgatatggtc
    22021 tgcgatattg cgtgagatag cggtatcttc ttttctcaat tcctcaataa cgtgtaacag
    22081 tcctctattt tcactctggg taacagaacg caccgctttt cgtaacacgg ggaatttttc
    22141 gccgtcccta gaggaaatac ccgtaaggaa tgttaagatg tcgattgcca gactttcagc
    22201 gtctttcaca ttcttcataa tcacaaatgg gtcaagaagt cctgcattgt ccttgtcgct
    22261 ggtaaggttt acgatattga tttcatgggc tatctctggg agcgtttctt tccagttgcc
    22321 acgctcactt ttagggtcta acagaagtgc ttgaccgcca aacagcaccg cataatatac
    22381 aagtagattg ttgcagaatg actttccacc gccaagactt cccacaaaag cagaagcaag
    22441 ggcattagta actgtgcctt ttactccctg cgaagctaaa gacggttgca ggtacacatt
    22501 tcttcccgta tcaacggaat agcccatata gatacccgtg ttttccccta actgctgggt
    22561 cgcaccaaaa ccaagcccag ccaagaagtc tgactttaca tactgcacat agtcattgat
    22621 atatcgcttg ctggcaggta aaaattcaga atgaagtccc aacatatcgc cagcaggacg
    22681 caccagtttt acattgaggt cgtcgtaaaa gtccttgact tcatcacaac ggcgtttcag
    22741 ctcgtcaaga tcgggtgcag acacccgtat gacgtagctt aacttataca tactttcttt
    22801 gctctggtct aaatcggttt ccagctcgtc cacgctgtct aaagcgtcca ccacatttga
    22861 gctggtttca cttcctgctt gataggcgtg attatccaaa tctttcaatt cctttttctt
    22921 gttgcggact gtcgttaatg ccttacggtt ctccacgatt tctacattca tagaagtatc
    22981 aacggggaat gtgaattgct gttgctggaa atagaagatt tcagaggacg gaaaatccaa
    23041 ctccccgaca attacattga cggtaaaata ggacacatag ctttccttgt cctcatgttc
    23101 caatcgtaaa taccgctggc tttcctcaat cacacacctt gtcggacgga taaggtcgta
    23161 gtattttatc agcgtttctt tcttcaattt cttctttggt agctggtact cataatcttc
    23221 ataggcgata ccgtccctgc cgtaaagatg ttccatgaga tacccaaaat catggatttc
    23281 caagcgacgc accttaaagc gacgggagat tttattttcc agtaactttt ccattttcat
    23341 gtaacggttg atttcatcat tcggcatgga aacaaaatca ttcatcagcg tgtggttcac
    23401 ttcatgaaga aattccttga acgtcagcca tgccgatttt ttgatgttct tcagattgag
    23461 ctgttcttcc gtaaccatga gcttaaagcc aagaaaaaag cggtagtcca cttgattgtc
    23521 cccaatcatt gatactaacg cttcggtctg ttcgtctatt ttttggactg ccacctcacg
    23581 aagtttgcct gttaccagtt tctttgactg ctcctgcata cttctaatgg agctttctgt
    23641 ggcaatctgc aacgcatgaa tttttccctc acgggactgt gcgattagct gtcgaaaact
    23701 gtcatgcacg ataaatttct gctctgcgga tagaaaagaa tagttgtacg gtatcatctc
    23761 atagtaggcg aacacctcat tgtccttgtt ccagacaagg ttattgtcaa tatattttat
    23821 cgggaacata gttcacactc cttactgccg tgattgtttc attcaatatc tgcttatgca
    23881 gttttacggc ttttcctgca taagtgattt taggtcgcaa ggcataggtt atctgtgatt
    23941 tcaaaaagct gtacggcttc tttccgtcaa aggttttctg cgacataaac caagtgagag
    24001 caacgggaat accgaagtat ttgagaaatg ctccctcaat catggaaagt gggggcatat
    24061 ccccaaacag aatgatgata aattccgtaa tcacaaacca tgtaatctgt gtaaaggtaa
    24121 cgggaaaggg taagttaaag tcattgattg catataagac tttttccacg ttccagatac
    24181 ccgtgtagct tttaatcttt ttcaagttct ttcagcccct ttcgtaagtt cttcaaagag
    24241 aaaaggacag ccattttcag actgtccctt aaagagaaat acgctgtcag tagcaacaat
    24301 gcttgttgct gatcttccag cgttaatatg gtatctcaca gatacctcgg cttgtttcaa
    24361 tgaatcgacc acccatatcc aaatctcgcc cgtaggcttc atagtcaata tagttctgta
    24421 agttagctgg aatgtcccca agtgcgttca attcgtctat gtagtagtag gcaacatctg
    24481 tcatggtttc acaatcggga taaaaataaa tatcgtcctt atgttccacg acttcttcca
    24541 gcgtcccata gtggctgata aattcgtcca gacactctac gatatagtcg ggaagttcct
    24601 ctatcatttc atacatttca ttgagttctt caatggaaac atattcccct atgtcgatag
    24661 gaaagttgtc ggtatcatgg acagcgtatt cttcgtactg ttcattcaag ccgatttttt
    24721 cttttacatc ttcctcgtca atagggaaag tgaaccacgc accgactaaa tatccctcat
    24781 tgtatctgcc aagattagca atataaaccg ccatatcatc aatcataagc accacctcat
    24841 ttcctattct ggcagttcaa agatactgtt ttccgttaca agatagcgtt tgttttcaag
    24901 taagtgagaa gcgtaggctt caaaatcgaa atagtatgct tgacagtcct cggataaatg
    24961 tttgaatgtc gggtcgttca tcagcttttg ccttgcaaca tctatcatgc ttttacagtc
    25021 gtgataaacg gtaatcacgt tcctgcaaat ataaagtgct tctaagttct caaacacggt
    25081 aagaagttct acatattctt cctgcatatc ggctgatagc tggcggtaca taaaatctaa
    25141 ttcgttaagc tggtacacgc tggtgtgttc gtggacttca tcagcataag gcaacacctt
    25201 ttcgataatg cggtaatctt gactttctgc accgacacct aacttttctt caaactctgc
    25261 aacatctatc ggtaagtcaa accagtatga tgttgtttct tcgccatttg ttcttcccgt
    25321 ttccaccagt acccttgttt cctgcattgt tcctcacgtc ctttctgttg tttcatcaca
    25381 tcttttaagg tctggtatga cataccaaac acatatttag aaaaatcttt caactgtttt
    25441 tcttcatagt cggcagggtt cagccgtttc atttcatgcc acaccttacg cttgtaagag
    25501 ggcaggtcgg aaatgtattc acagccgact tttgcctgca tatctttaaa aatatcgttc
    25561 atttcatcac tccaatctga aatttaggta agaaaaaagc agtcaatcct aagactaact
    25621 gctttgtgtg taagtatgaa tttgtttaag tgtaactgat ttaccatgtg tactcgtaaa
    25681 gtttactgta tgtttgactt ttaaataaat atatacaatc aatcatttgt aacttgccgt
    25741 cttgttcaaa tttacttatt atcagattat cttcatagat atatgctaag aaagtatcat
    25801 actctgcata gtatttttct ctaaaaagat ttgactctct aaaatctttt tctaaagcaa
    25861 ttcttatact gtctaaagca tatttaaagt tataacaagt attaaaacta aacttgtatg
    25921 gaaaaaagaa aaataagttt ttcttgaaag taagcaattt cacataggtt tgtatatcaa
    25981 attcaatcgt tccatactca tatttctctg taagacaaga atgtaaactt tcacaatcat
    26041 aatcccgcaa cgaagcaaat aacctagtta cagtcatttg tgtatttggt cttctcggtg
    26101 gacacttacc aattacacca tcacagaatt tctgtatttg accagtaagt tcttttgaag
    26161 catgaagtct tgttgtgctt tcaagtaatt taaagtccat ttgataatta ggtgagttac
    26221 aatcacattc tccatgattt tcagatttag gtggtacata ttctgcaaaa tttgattttt
    26281 ctctaaaata tattgattta ttaactaatt ccaacaagta tttttcataa ttacaatttt
    26341 cttcatcttt aataaaattt ttgtttatga atactgcatg taaaatatcc actctcattt
    26401 catctgaaat cataagcact tttattcctt tcaaaaaata atttatcgta cccacattat
    26461 aacattctca tattacagca acaataaaat tcgttatgct ccaataatct tattgaacag
    26521 ctctaacagt acatctttca caccgccagc gttgaatact aagccgacag caataagggc
    26581 aactaccaag aagccaataa gttttgaaaa ctcacgcttg aaccctaagt agataccgat
    26641 aaccacaatc gccataagca ctaagctctg tgcatttgat aaaaaccatt gatacaaatt
    26701 ttgtccgaaa ttcatttaat tgtcctcgct ttcttctaat tgtttcattt catagtcagc
    26761 ttctttgctg atactcaaaa tacacatcaa gactacgccg atacccattc ccatagatac
    26821 cagcaggaag tcttttacta attcccacat tttttatcac tcctaacttt ccaccaaatc
    26881 ttttgcaggg gtcgtctgct gtttgattat catttcatgc ttttcactca agattgcctg
    26941 cttttcaatc gtttccatgt agtctgtacc gtttccctta tcaatctttt tcagcatttt
    27001 caaggtcggt gctacctgtc tttgtaccca acgcaatgtg cggtctaagg tgtaaggttc
    27061 tggttttgtc gtcagtttca gactttgccg attatcgcca ataaaccaag cccagcggtc
    27121 attgagtttc cagtcatttt tccgcttgtc tggttcttca tcaataaacc gcacatactg
    27181 gttgatgata gaaaaagcgg tctgctctgc gtcatagtag gtcagcaaat ctcgtactgc
    27241 atagtaagca cgttcatttc taagccgtat ctcaaaacgg ttgataatgt cggcttcttc
    27301 cagaggtgta cctaatttga catactgctc atagtccttt tcatagatac agaaatacac
    27361 atctgatttc agcgaaccaa gataaagggt actccccata tattctctgt cgtcctctct
    27421 gtgcttgata agctcgcccg actggtaata cttatagctt ctggactttc cgatatattc
    27481 ccgtttcctg catttctccg caagctctgg aatatccaaa atgcccgtat ggtcgttgat
    27541 agcaaggtcg atacgcttca taatgcctcc gtctatgagt gcgtccataa gaaagtcata
    27601 ccagcttctt tgctgtgcca gcaggtaact ttcaaactgc ctgcaacccc gaccctttaa
    27661 ctctaaaagg acaccttttt cttcgtcagc cgacgtatag ataaagatgt cccctaatga
    27721 ataatgctcc gtataactgt aatgtccgta atcttcatgg agcatataat tgatattcag
    27781 ttttaatatg tctttgatga tgtgctgtat atccagcgtg ggaaaacgaa ttttcacata
    27841 atcaaacagc atggtaagcg gtgcttctgg attgaacctt gccagttctt tatgcaaagt
    27901 ttccagaagt tcttttgacg gcttcatttt tccgctttct atcttgttta gatattccct
    27961 tgtgatacca gaagctaccg ccagcctgcc ttgtgagata ccgtaagcaa tccgcttctc
    28021 ccgtaattct tttatccatt gttcttcatt cagaaccata cccccaatct gtcaagtgtg
    28081 aagtttttta aggcgacttc acagccgatt tttgctagga aaccatgcca gaagccttat
    28141 gtttcctatg tttttttgtc tattgtctat ttgcaaacgt acccctctgt tagataccga
    28201 ggggttttac ctgctggcgt ggcttacgcc acaccagcaa cggctagtcc gagccgtcgc
    28261 ctttcgcttc gcacgtcgcc gtcccgtcct gccttgcttg tgcaagaaaa ccgattgtct
    28321 gcaaaaaatc atgtcctttc gggactaaag gcgtgtaaaa ttcacttatg acgcttgttc
    28381 ccacatcaca atagccacgc cctttgatac gcttttggaa aaactgcttt ttcacatctg
    28441 aaccaaacag cataccgtaa cctaactcgc tgatacgccc caatcccaca cgtaaattga
    28501 agttatctct gataccgtcc gagaaatatt ttgcgtctgg acgctggcag gcaacgataa
    28561 ggaaataacc tgcttgtcgt cctaacatga cgattttctt taactggcta agtaagccca
    28621 cactttcttt tgtccccagc atttcaaaaa acgccacata ttcatcaaag ataagaaagc
    28681 agggtggcag tcccagatag gcgtaatttt cgcccgtctt atagttcggg tgtcgtttca
    28741 tttgctcgct tcgctgtacc atgccctcat aaaaagcatt gacacagtct atcatttctt
    28801 ctttggtgtg atacacgttt cccatgactg ttcccaagtc tgctaagtcc gcatttttcg
    28861 ggtctaagat gtaaaggaca gcgttggtat gcagtagggc ttcaatgagc gtcagaagaa
    28921 aatatgtttt accgccacca gtcccaccag caatcagagc gtgagggagt gcgtcatatt
    28981 cccagacaag atttttcatc agtctaagac agccgttctc tgcccgtact tcatcaatgg
    29041 taatgcggtt cgctatcata tcataaagca gggtatattc gatatagccg tcatgcaggg
    29101 tcttgtcggt cagctcacaa tacaagccac tttccaattt atcctctaac cgtaaaagct
    29161 ggtcttgata tttccccagc gtaatttcac agcggatatg aagcagtccc ttttccattt
    29221 gataataaat ctttggaaac cagacgattt tttcccttga tctgctttgc aggtcagtaa
    29281 aaaaaacgct gtcttgtacg gtatcagctt cataccactt gttttccagt atcattcgtg
    29341 ccagcttttg acggtgcagt agctttttga aactgtcgta acagaagcgg taatacagaa
    29401 aagcgaccaa tgcacaaaca ccagtcgcta tcagtatggt tatgaagttg taaagggaaa
    29461 gcgtaaagcc attgtctaat aagctgaaat gttcccagtc ggtacgcatg agctgttttg
    29521 tatttaatag caggataacc gctacgaaca caaacaggag tgtccccatt gaaaagcgat
    29581 agacaaggtt cttatcgctg gctctgatac ggtgtccttt gtttaaaatc attctcataa
    29641 atcaatcact cctttctggg tatgaaaaaa gcagtcaatc gtatagacta actgctttgt
    29701 gtgcatggat atgaagttgt caaactggaa tttaacgttc actcatatat tgttttattt
    29761 catcagaagt caattttcgt acttctgtat gtggatattc tttatactct ccaacattaa
    29821 acacaggtgt catttgtttg catttcttgt ctttattctt tttcaagaat agatgtaaat
    29881 cctcattccc ggcgaatatt ttataagata ctctgccttt actgttctct atctcataaa
    29941 taccacatga acttttcgtt gtataatcgg ctgttcgtaa ataaagtttt gcaatttcca
    30001 atgacttaaa aggaaaattc catctttgta atcctctgtt tgggtcatgt tcaatacaaa
    30061 tgcaacgtcc acaagttcca caaatatatt gagtgtggtt ttctaagcag tccaagggat
    30121 ttagtaatgg tgttattcta ttttcatcag agtaacattc ttcacacatt tttcttttcg
    30181 ctccttatca aattctaatt tgtcgctttc ttcataccca tattataaca gttccgtata
    30241 tcctctacaa cgaaaatcta ttcagcaagt ctattttttc ggttgtccct gcggtgtatg
    30301 gttctgtgaa gtgcctgtgt tctgattgcc tttattcttc agtacaatat cttctgcctt
    30361 tacataccag tccacatctg cacccgtata ggtttttcgt gataccgtat cggctacggg
    30421 attgacaagc tccacgactg cattgtaagg aaattccctt aacggtactt ctggcggtac
    30481 ggacacgggg ataattccac catgcagact gcacttcaaa tcatagatac gctttttaag
    30541 ctgggtactc ggtgtcccgt cctcattttg caagaacaca tcacgcaccg ctgtaaattt
    30601 taattctcca aatgttttct ctttgtcaat gacaaatcca tttgataatc tcataaattc
    30661 ttcactcctt tacttttctt caactgctac aatatcatca gctactaata cataatcggt
    30721 atgtcccaca tccccgattg cgtaccctct gccgtataac ttcggattga caagttttac
    30781 tttctgttca tacttgaaat gcttttcgcc agcctgcacg ggaatttcca ccacaacatt
    30841 ttcgcctttc tgtacgtcag aataaaggtt atagcttcgt ctggctatga ccttgcggtt
    30901 gtttttatcc ctttcaaaga taggctcgct ttcgcctgca aattcaagag ttccaaaaga
    30961 ctgtgccata tctggcacga catatttcat ttccatattg ttttacctcg tttctttcta
    31021 tatttgataa gtttatgatt gtaatttttt attctggctt gtctgccgtt tgacagtttc
    31081 ggcggagaac gtgatttttc tttcatcata ccgttaccgc cttatgattt tttcattgta
    31141 cggcgtttgt aaagatatgt tccagccagt ccaccagcag atgtaagcaa catcacaaga
    31201 aatgccatga catttgtact gtcgcccgtt ttaggactgt cgctaggtct gtttggcttt
    31261 tctggtgtct gcggtgtttc gagtttctct gggacttctg gtttttcatt aaaagtaatc
    31321 gtctgtccct cgtcctcaat atccttatgc tcggtaactt tcttcggctc gtctggattg
    31381 cttaaatcat acaattcttc aaaagttaca agctgtttgc cgtcaaggga agtagcgtca
    31441 aaggtaaagg caacttccgc tttcatagtt tcgctgtcag cggtaaaggt gtaatcactt
    31501 tccacacgtt tcccattgat aagaagttct gcattttctt ctttcaacat ctgccagcct
    31561 acaagtttgt actgtgtacc gacttccaag ccctctaagg ttacggtatc aatgattgta
    31621 acgtcttttc cagcttcaat ctctttgttg ccgtctttgt cggtagcagt cgtatgtatc
    31681 ttgatgatac gttctgtaat caatacagtt tgtccgtcgt cctcaatgtc tttgtgttcc
    31741 gcaactttca cgggttcgtc tggattgctt aaatcgtata attcctcgaa tgttaccaga
    31801 tttttaccgc caagctcgga cgcattgaat gtataagaaa tttccacttt catttcttca
    31861 tcatcagcga taaaagtata gtcattttcc acacgttttc cgtcaatgat aagctctgca
    31921 ttttcttcct ttaacatctg ccagcctttg agctgatatt ttgtaccttt tgtaagcccg
    31981 tctaatttga cagtatcgac gattgttacc tcttttcctg caaggatagt cttttcgctg
    32041 tctttgctgg tcgctgtggt atggatagag atttcttttt cgtattcatc agtcaaagtc
    32101 cctaaatcaa tcacaaggtt atttcttgat accacgattt caaaaggtgg gataagttca
    32161 aagcctttgt tactatcgca acgcatttct tcaatgatgt aagtatcata aggcaatgca
    32221 cctttgctgt cgtctgcttc agaagttcca aaccacacac cgtcctcgct ggtcttgcct
    32281 gcgttagtat tgtgcttatg agaagtccat tcagcagaag tggagaattg cccgttatca
    32341 tcagtaacta caacatgact ttctcccgtc gtcttgcttg tgatcctaaa gggaacatca
    32401 gcaagacgct tgtgtgtgcc tgcaccgatt ttcactccct caatatcccc acgcttaatc
    32461 tgattgtaga tagagtgggc ttcgtcggtt aagtccacga tttttccatt ctctgtgatt
    32521 gcaaagtcaa tcggcttcgc accgtctgtc aagtaaccgt cgggagcttc actctcaacg
    32581 atacggaatt tcccataagg taaaaggtca gcagaagtag aagccacacc ctcaatatct
    32641 gtacgaattg tctttaccac ttcatttttc ttatatagct tgccctcaac caaaacagaa
    32701 ttgtcattta aggaaatgac gtcaaaagca gtatctttca aagtggcact tccttgtggt
    32761 ttggtatcgc tcgtttctaa atctcttttc tgaattttca caccgccacg gataaccttg
    32821 tctgatacgg aaaactggtt acttcccgtc aatacagcaa ggtcgccgtc ctctgtaatt
    32881 tgtgtaaggt ataagccttt tatctgttca gacttatcgc cagcctgcat atatgcaccg
    32941 tccagcaagt agccgtttgg agcttttgtt tcctcaacag atagtgttcc taaaggaaga
    33001 atcgctttgc cgtcctgcat atagaagctg tcgccagata ccttgtaagc gtccgctaat
    33061 tttgtgatgt agtgagttgt cccgtcactg tctgtttcag cgattgtctt tgtaacccat
    33121 gtacgagtag cttcggcagg gagattgtct ttgttataga agccagcata atacttccat
    33181 gtaaactctg caccctctaa agaagcgttt ccctgcggat tgtctttctg cgtttccata
    33241 tcaatcttga aaagctcaat caaggtgtcc gttacttttg gtgtatctga tacgttcaag
    33301 gtcgctgtct ttccagcttc aacttttaag gaatacacgg ttgtatcaac tttatatcct
    33361 gctggtgcgg ataattccct aatatagact gtacctgctt ttacctctac aacatctgta
    33421 tttccgtttt tatcagtcgt aagggtggca agctgtttcg tgcagtcctt atcagaaaag
    33481 acaccatagg tcgcaccagc gattgagtaa ttcccgttac cgtctgtaat gctggtatta
    33541 ctggaagtct tttgcagttt tgcatttccg acatttaact tcgcccagaa ttgtcccaat
    33601 tcctgcccct cgccagagta gatataaccg ccacattcat agcgtccttt attttctttc
    33661 acaaaggctt tagcaccagc gaaaacttcg tcctgagttg cttttggtat ttcatcataa
    33721 gaagctcgca cgttgtcgca ttgccagcca agatgtacgc tcaatctctg ccagacaaca
    33781 cattgttcca acagatagac ttgcttgtag ttcaattcct tgtgtgcttc gccatactgt
    33841 ttgacatact ctaaggataa cgccacatct gaaatctggt cggcactcat acgggtgctt
    33901 gcgtcagctc tggtcttgta gccattctta aaat
//
LOCUS       DAMKMI010000028        32860 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMKMI010000028 DAMKMI010000000
VERSION     DAMKMI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.029"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            9..1865
                     /locus_tag="PC429_001269"
     CDS             9..1865
                     /locus_tag="PC429_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF3846784.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    1948..2188
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2254..2489
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2574..3791
                     /locus_tag="PC429_001270"
     CDS             2574..3791
                     /locus_tag="PC429_001270"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF3846785.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            3908..4402
                     /gene="cdeM"
                     /locus_tag="PC429_001271"
     CDS             3908..4402
                     /gene="cdeM"
                     /locus_tag="PC429_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF3846786.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGENRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4667..5932
                     /gene="hisD"
                     /locus_tag="PC429_001272"
     CDS             4667..5932
                     /gene="hisD"
                     /locus_tag="PC429_001272"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF3846787.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6135..6887)
                     /locus_tag="PC429_001273"
     CDS             complement(6135..6887)
                     /locus_tag="PC429_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3846788.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7005..7274)
                     /locus_tag="PC429_001274"
     CDS             complement(7005..7274)
                     /locus_tag="PC429_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846789.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            7749..8843
                     /locus_tag="PC429_001275"
     CDS             7749..8843
                     /locus_tag="PC429_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF3846790.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            8845..9795
                     /locus_tag="PC429_001276"
     CDS             8845..9795
                     /locus_tag="PC429_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF3846791.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKVDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            9788..10972
                     /locus_tag="PC429_001277"
     CDS             9788..10972
                     /locus_tag="PC429_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846792.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            10985..12481
                     /locus_tag="PC429_001278"
     CDS             10985..12481
                     /locus_tag="PC429_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF3846793.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEECMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVND
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            12504..13454
                     /locus_tag="PC429_001279"
     CDS             12504..13454
                     /locus_tag="PC429_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846794.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            13475..14437
                     /locus_tag="PC429_001280"
     CDS             13475..14437
                     /locus_tag="PC429_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF3846795.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            14809..15441
                     /locus_tag="PC429_001281"
     CDS             14809..15441
                     /locus_tag="PC429_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3846796.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            15852..15944
                     /gene="kdpF"
                     /locus_tag="PC429_001282"
     CDS             15852..15944
                     /gene="kdpF"
                     /locus_tag="PC429_001282"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF3846797.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            15985..17667
                     /gene="kdpA"
                     /locus_tag="PC429_001283"
     CDS             15985..17667
                     /gene="kdpA"
                     /locus_tag="PC429_001283"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF3846798.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            17692..19749
                     /gene="kdpB"
                     /locus_tag="PC429_001284"
     CDS             17692..19749
                     /gene="kdpB"
                     /locus_tag="PC429_001284"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF3846799.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            19768..20394
                     /locus_tag="PC429_001285"
     CDS             19768..20394
                     /locus_tag="PC429_001285"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF3846800.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVEDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            20631..21539
                     /gene="cysK"
                     /locus_tag="PC429_001286"
     CDS             20631..21539
                     /gene="cysK"
                     /locus_tag="PC429_001286"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF3846801.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            21566..22156
                     /gene="cysE"
                     /locus_tag="PC429_001287"
     CDS             21566..22156
                     /gene="cysE"
                     /locus_tag="PC429_001287"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF3846802.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            22316..22504
                     /locus_tag="PC429_001288"
     CDS             22316..22504
                     /locus_tag="PC429_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF3846803.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(22558..23427)
                     /locus_tag="PC429_001289"
     CDS             complement(22558..23427)
                     /locus_tag="PC429_001289"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF3846804.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDVINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(23624..24868)
                     /locus_tag="PC429_001290"
     CDS             complement(23624..24868)
                     /locus_tag="PC429_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846805.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYNMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            25159..25470
                     /locus_tag="PC429_001291"
     CDS             25159..25470
                     /locus_tag="PC429_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF3846806.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      25563..25655
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            25755..26549
                     /gene="thiD"
                     /locus_tag="PC429_001292"
     CDS             25755..26549
                     /gene="thiD"
                     /locus_tag="PC429_001292"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF3846807.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            26573..27361
                     /gene="thiM"
                     /locus_tag="PC429_001293"
     CDS             26573..27361
                     /gene="thiM"
                     /locus_tag="PC429_001293"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF3846808.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            27384..28016
                     /gene="thiE"
                     /locus_tag="PC429_001294"
     CDS             27384..28016
                     /gene="thiE"
                     /locus_tag="PC429_001294"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF3846809.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKQYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            28155..28457
                     /locus_tag="PC429_001295"
     CDS             28155..28457
                     /locus_tag="PC429_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846810.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(28523..29272)
                     /locus_tag="PC429_001296"
     CDS             complement(28523..29272)
                     /locus_tag="PC429_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846811.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(29266..30228)
                     /locus_tag="PC429_001297"
     CDS             complement(29266..30228)
                     /locus_tag="PC429_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846812.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(30232..30690)
                     /locus_tag="PC429_001298"
     CDS             complement(30232..30690)
                     /locus_tag="PC429_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF3846813.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            31046..31402
                     /locus_tag="PC429_001299"
     CDS             31046..31402
                     /locus_tag="PC429_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3846814.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            31474..32190
                     /locus_tag="PC429_001300"
     CDS             31474..32190
                     /locus_tag="PC429_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846815.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            32183..>32860
                     /locus_tag="PC429_001301"
     CDS             32183..>32860
                     /locus_tag="PC429_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846816.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYI"
BASE COUNT        12658 a         3594 c         5793 g        10815 t
ORIGIN      
        1 gttagaatat ggatacccat aataaatatg taaattttat aaaaaatata cctgtacctt
       61 ttttatattg tagaattgta aagagacaag aagatataga atatcgagta gaatatataa
      121 gtaagggtat gggcaaagta ttacaattag aagaaggtat ttgtgataaa aatatactag
      181 atgtattgcc tgtatttaag tctaaaaaat atttcaaaga gttgttctca aatgaagtgg
      241 attgtataaa aaggtacatt ccgacactta aaaactggat aaatataaaa aaacagataa
      301 taggggattc atacataatt ttgtattttg gtaaaattgt atttgattat agacagataa
      361 tcgattcctt tgataaaaag gaaaaagtag cttatattaa agacgaagaa ggcatatata
      421 tagattgtag tgaaaattta ataccaatat taaataacaa cattaaaaca acgaaagata
      481 tctttggtaa aaatgatata gaagtatggg gagagaatac aggaaaatta tttagagatg
      541 attatagaga aggagtatct agtaaaaaaa gatttttaca aaatcttttt gaatatgaag
      601 aaacattttt tatggttgaa aaatattttt tgtatgatga agacgaactt ttaggtacta
      661 tagggatagt agacaatata atatatagtg gttacagtaa tagaaattat aactcaaaag
      721 atttaatgaa gatgatagaa cactcaattc cagagaatat gttttataaa gatgtgtatg
      781 gaaattatat tggatttaat tcaggttttt tgaatttagc ttgtatgaat aaagaagaat
      841 tattagggaa gaatagttat aaaatatcag aagaagaagc tttaatagat aagatatttg
      901 aaagtgataa gggtgtagtt gaaaataaaa aagtagttac atttgagtta aatataagta
      961 tgaatgatga aaacaagtgt attgagatta caaaaaggcc gttttttgat agctatggaa
     1021 gtgttattgg aataattgga acagcaaggg atataagtag aagaaaaaga ttagaagagg
     1081 aaatggataa aactaggatg gaattttttg caaatttatc ccatgaactt agaacgccta
     1141 taaatttaat atcatcttca ttacaagtga tagaaaaaaa agaagcagat ttaatagaat
     1201 caaatgatac tttaaaaaga aatctaggta taataaaaca aaatggaaat agaatattga
     1261 ggttagtaaa taatgttata gactttacaa aaatgcaatc aggatattta gattttaaac
     1321 cagaagaatc tgatataatt gcatttatag aggagatatg tatgtctgta gctgattttg
     1381 caagccaaaa taatatacaa cttacatttg atacagaaat agaagaattt tcaatgttat
     1441 ttgattcaga aaaattggaa agaataatat taaatttact ttctaatgga attaagtaca
     1501 ataagaaaga tggaaaaata aatatatttt tatatgtgaa agataatgta tttaatatga
     1561 aaatatctga tagtggtata ggtataccaa aagaaaaaat agataaaata tttaataggt
     1621 ttgaacagat tgacaatgaa ctttcttaca gagttaaagg aagtggtatt ggactttcat
     1681 tagtgaaatc tttggttgag ttgcatgagg gaagtatatc tttaaaaagt caacttggta
     1741 ttggaagtga atttatagtt tcattgcctg ttagaagtaa aaataatatt gaaaaatata
     1801 atcataaaag agaaatcagc aatgaactaa gtaaaaaatt agaaatagag ttctcggatt
     1861 tataagttta aatataaaaa ataaaatttt aaaaaagtat tgacaattga tttagaaggg
     1921 tttaaaataa attacaacat aataaataaa aaggcgatga ataggaagag taaatctaat
     1981 tcgattacca gagagaagag tccgttggct ggaagacttt ttaaattaga gggattgaaa
     2041 actacctagg agctactttc tgaagtcatc tgcattagta gattttagta agaatagtcg
     2101 ttagctgcgt taaagtgtaa agtgagtttt catgacatat gaaaactaaa ttaggtggaa
     2161 ccgcgggaat aatactctcg tccttatgtt ataaggatga gagttttttg ttatttaaaa
     2221 atataaacag taatcataat aattatagaa aatataaaag caatgaatag gaagagtaaa
     2281 tttaattcga ctatcagaga ggaaagttca caggctgaaa gactttctaa gtttgagaaa
     2341 ttgaaaacta cctaggagct gtggtctgaa gttactttaa aaagttttag taagactaac
     2401 cgttaactgc gttaaagtgt aaagtggatt ttatcaataa atgataaaat caaattgggt
     2461 ggaaccgcga gtatagactc gtccctttat atagggatga gtctattttt attgcaataa
     2521 aagattgaaa ataaaaaaaa atcggaaaat tataaattta ggaggaaaat aaaatggaga
     2581 ataaagtgaa aattggagta ctaggatatg gagttgtagg aagtggactt atagatataa
     2641 tagataataa taaagaaaaa agaaatatag aaatagttgg aattttagtt aataatttgg
     2701 aaaaacataa ggataaaaaa tattctaata taataactaa taatatagat gatatattta
     2761 ataaagatat agatatactt gttgaagtca tgggtggttt agaaccatca ttatcttata
     2821 taaaaaaagc attaaacaat aaaattcatg ttgttacagc aaataaagat ttgttagctg
     2881 aatgtggaga tgagcttgca aaattagcaa gtgaaaataa agtaagtatt aaatttgaag
     2941 cttcagttgc aggaggtata ccagtattaa aaccaataat agaatcacta gaaggaaata
     3001 atatagatag tataaatgca attttaaatg gaactactaa ttttatatta tctaaaatgt
     3061 atgatgagaa tctatcttat gatatggcat taagacaagc tcaagaatta ggttttgctg
     3121 aagcaaatcc agagtcagat gtattagggt atgatgctgc tagaaaacta tcaatattat
     3181 caactctagc ttatgacaat agagtatatt ggaaggatgt gtatttagaa ggaattacag
     3241 atatagatga aaaagatatt gaatatgcaa aaaaattaaa ttgtaaaata aaattgattg
     3301 gtcaaagtaa gtatgaaaat gataaagtaa gtgcttttgt tagacctgtt ttagtagaaa
     3361 aagataatat ccttgctaga atagacaatg agttcaatgc tgtcatagtg aatggagatt
     3421 cagtaggaga agtttctttt gtaggtaaag gcgctggaag tttagcaaca ggaagtgcgg
     3481 tttattcaga tgttatagat ataatagata atagagtttc aagtatagat tcttttacta
     3541 aagataaaat acaagttaat aaaatagtta gagaaaagtg tggagcactt cttagattta
     3601 aaaaatgtaa taaagatgaa attctaaata tagttgagaa ttgtttagta aaattcgata
     3661 ttttaaatga tgatgatgaa cttgctatta tggtttatgc tgattctgaa tatgaaataa
     3721 acaacagttt atgtctaata aaagataaag gatactgcga aaaaatgaat aaaatgttga
     3781 aaataagtta aaaaagaaaa aatataagtt tatatgtaac atattttgac gattttaata
     3841 ctatatagta tgagatgaaa caaaacatct cataaaaaaa ttaaaattca agggagagat
     3901 atgttatatg gaaaataaaa aatatgcaaa tggtggttat tcagaagact ggtatgaaag
     3961 aggagaatct acagctaaat ggttccagaa tgatagagaa gaatatgaaa gagcagcata
     4021 cgatgaagat agagaaagaa gaggctcaaa ctgtggatgt tcagattcag gagaaaatag
     4081 acctagaaac tgtgaaagat ttagaagaga agcagagata agagaaagag aagcaagaga
     4141 agcattctgt gaatcttcag agagaaagaa agaagcacta gcatatgaat gtgaagctag
     4201 aaaattatgg gaagaagcag aaaaatactg ggatgaatat tcaaaataca actataaagg
     4261 aatcgaatat ttagcagaag ctgctagatt atttgatgaa ggtatggaat gtgaagctag
     4321 aagaaatgga aataatggag gaaacaataa taattgttgc cataaatgcc ataaatgtaa
     4381 ttgtaactgc tgtagaaaat aaaaaaatta attgacaaat caatagttaa aatatatcat
     4441 attatgtata ataaagagaa aattcagaaa aaaataatga ttagaagaaa tataagtata
     4501 tttattagac aaatagtgct cagttagtga ggtgagtaca atatgattaa actcccatcc
     4561 tctagctttt gatagagggt gggagttttt atttatttat atatcttaaa tgaggataat
     4621 attgtaaaat aaatgagtat gagattgtaa aattaggagg taaaaaatga agagtattaa
     4681 aacatcaagt atgaaagaaa aggtagatga aaagatatta agagagcaag taagttctat
     4741 aatagaagat ataagaaata ataaggatgt tgcacttaaa aaatataatg agaagtttga
     4801 tagaaataca agagatgaat ttagaattac taaggaagaa ataaaggaag cttataagca
     4861 tgtagatgac gaatttataa ataacttaaa gattgctgcc aaaaatataa gagaatttgc
     4921 taaagctcaa aaactaagtt ttgaaaaccc ttttgaaaaa gaaatttatc caggtgtaat
     4981 cttaggtcaa actaatattc caatagagtc atgtcttgca tatgttccag gaggtcagta
     5041 tccattgttt tcaacagctc ttatgcttat aatacctgca aaagtagcag gagtaaaaag
     5101 aatagtggca tgttcaccaa ctatgaaaaa tactgaaaag ataaatccta agacattggt
     5161 agctatggat atagcaggtg ctgatgaaat atatgcaact ggaggagttc aagctatagc
     5221 agcatttaca tatggaacag aaaagattaa tccagttgac ataattgtag gaccaggaaa
     5281 taagtttgta actgaagcta aaagacaatg ttatggacaa gtagggattg attttgtcgc
     5341 tgggccaagt gaagttctta taatagcgga tgaaacttca aatccagttt atattgcagc
     5401 tgatttatta gcacaatgtg agcatgattt aaatgcaaga ggaatattat tgacaaatag
     5461 cttggaagta gcacaaaaag ttgaaaaaaa tatagaaagt atgctaaaag atttgccaac
     5521 aaaggacata gcatatagtt catgggaaaa taatggagag ataatattgg ttgatgatat
     5581 ggaagaagcc attaaaataa gtaattttta tgcaccagaa catttagaag ttgcagtaaa
     5641 tgagtataat gatatttgtg atagattaac taactatggt tctttattta ttggaaatct
     5701 ttctgctgag gtgtttggtg actatgtttc aggtaccaat catacattac caacacttaa
     5761 agcatcaaga tatactggtg gtgtttgggt tggaactttt atcaaaactt gtacaaagca
     5821 gatatttaat gaaaaagcta tacaatcatt agcacctgta gctgaaaagc tggcaaaaga
     5881 agagggatta tatgcacatg caaaggctgc tgaggttagg tttaaaaagt agtaaaaaat
     5941 tttattttgg cagtcgagat ttagtgttag actgagataa ggaaaacgct gagaacccga
     6001 ataaatacta tattttgtgg gcctcggcgt ttcttttata tctgtatata ataggataat
     6061 actacctgtg tgtttaaatg ctagcttaat atttcttttc taaataatgt ttatgaaata
     6121 gttaatattt ttagttaata cttgaaaaaa acttttgcta actgtgcaaa ttcatctgca
     6181 ttcgtcaata tagcagggtg tcctccctga ctaaaaatgt gaatacttgc ttttgaaata
     6241 aaagttgcca tttgtttgta ctcttcttca agattatgac gacacatttc gtcttcctta
     6301 cttcctgtaa ataaaattgg catttctaat ttacacaatt ctttatgaaa caaagggcgt
     6361 ttctcgtttg cacattgcaa tagagctttt gtatcaagat taacaacatt ctcccaatct
     6421 tttccatgac accattcata gaattttcgt gcttgaatat cttgctttga tttttccctt
     6481 ccagtgatta gattatctat gaagttatca ttaagagtac gtccatcaaa gctatctgct
     6541 ataattgctt caactaaatc gggacgttct aatccagtat taacagctgc ccatgctcct
     6601 ccacttgttc caattaagcc aactttacca caattcaaat gttcaatcaa tgctattgtc
     6661 tgtaaggctt catcatgcca tatgtcagga gaaaattgag aaactctatc cgactgtcca
     6721 ttacctaaaa aatcaattaa gatacatcta aaattttcta catataatgg cacaagtgct
     6781 ttaaacatat tagaagaacc agtattacca tgcaggaaaa ttatcggttt accttgtcca
     6841 tattctttat aaaaacaaga attaccttga tactgaaaat atgacatata ttttcctcct
     6901 ttttatttgt taagttaatt ataacaatat aggtatgaat gaaaaacctc atgttctttc
     6961 ttagaataaa aatgtgccac gcataaaacg tgacacattt ctaattataa attttgctgt
     7021 ttaagtattc tttgtatgct tttctctgtc aaaaagtatt tctctgataa ctgcttgata
     7081 ctcattccat tttggaaatt tatacagatt tgagaattac gagaagctaa gacttgtttg
     7141 gtgtttgtgt tttcacccca atgctttttg tgctcttgct ttttgggaat ataaatcact
     7201 ttaccatcta tatattcttg cacttgctca atcagttctt taggcagtaa gtcaatcgaa
     7261 ttttgatagc tcattttgca cctcctaaat aattattctt taggattagc aaaggctact
     7321 gccactcaca attaaaaata aaatttttta ttatgtacaa tagcctttgc tatgcacaag
     7381 ttccaattcc cataaactaa atactccttt ctggtttgaa aaattcatct gaacattgat
     7441 ttcgctttta agatagagtt caatgacaaa tttacatttt tattatatat attcagatag
     7501 ataattgcaa ctgtctttgt atgaggatat tttattatag tcaatactat aatatgtatt
     7561 tatatcatca aactaatatg aacagttgaa aggaatatga acaataaaac ttagtcagga
     7621 agaataatta tgaagcagac aatagagaaa tttgatttta aagttttatt gaaaatttat
     7681 tgacaaattt aacttgtaaa tattatacta aacttaataa ataaagttta taaagtaagg
     7741 tgttgtctat gaaaaatata gttggaaaac cgcaaacgat aaaaaaagta aatgaagatt
     7801 taataaaaaa tattataaaa aatgaaggtc ctataacaaa accagaaatt tcaaacatta
     7861 ctgaattgag tttagctaca gttaataaga ttgtagagca attagctttg aaaaatgaag
     7921 ttaaagtaag tggactaagt gaatctactg gaggaagaag agctcaatta tttgaaataa
     7981 atgcaaactt agaacacata atagctttat attattatag aaattgttgt ataggggtag
     8041 ttgccaattt attaggtgaa ataatttatc aagaagaatt taatataaga atggatacat
     8101 atgaaaacgt taaccaagat actttttttg ttatagatac tctaataaaa aaggaaaaag
     8161 gaaacaatat aagggctatt ggaattggtg tcccaggagt tgtaaaaaaa ggtgtagtta
     8221 aaaatatacc aactataaaa agttgggaag gtataaattt agaaaattta ataaagagta
     8281 aatacaatac taaagtgttt atagaaaatg atacaaatat tactactatt gggatttatc
     8341 aaaaacagta taaagacaaa tataaaaata tggctcttat gtatttagaa gatggtattg
     8401 gttcaggtat agttataaat ggtgaattgt atttaggttc tacaaatttt gctggagagt
     8461 taagttatct aaatatagaa tttggtaagt ataactctaa caacttaaat cttgaagatt
     8521 atattatgag tttaagaaaa agatatttag aaagtaaaga taataaattt aagaaagaaa
     8581 ttttatctat aatatcaaac tttataagga ctcttgtatg tgtcctaaat ccacaagcta
     8641 tgattataca gtgtagtata ttaaccaaga gtgacatgga gtttatagaa aaagatgtaa
     8701 gcaataatgt tggagaagaa aattttccta aattaataaa agtagagtca ttgagagaag
     8761 gaagtataaa tggagtaatt agtatgtgtt taaaagaaac tgactatcaa tattccttat
     8821 ctaataaaaa aggaggatat taatgtgaaa tatttggttt caatagatgg agggggaaca
     8881 aaaactaaat tttgtgtaag tgacttagat ggaaacatac ttaaagaaca taccactggt
     8941 tcaactaatt ataagtcagt tggaattaaa aaaacatatg aaaatattaa taatggattt
     9001 aaaaaaatat taaaagattt atatatcgat tatgacgata ttgagtacac tgtatttggt
     9061 atatctggtt gtgattctcc taatgattat aagattataa tggatgaaat attaaaaatt
     9121 gggattaaca aagaaaaaat atatctggca aatgatgcag ttttagcttt ttatgcacaa
     9181 gcggattccc ctggtttagt aattgtggct gggacaggtt caattatact gggaattaaa
     9241 gaagatggag aaatatatag agttggtgga tgggggtata attttagtga tttgggttct
     9301 ggatatgata tagggagaaa attattaaag aaagtattgc tttattgtga tgaatgtcat
     9361 gagtatagtg atttatttag ttgtgtcttg gatttctttg gagcaaatag ttttgaacaa
     9421 cttgcatata tgattactga tataaacaat aatgttgaaa tagcaaactt agcttcatta
     9481 gtgattgatt gtgccgaaca aggtgataaa ttggctattg aaatattaag agaatcatca
     9541 actgaactat ctaaattagc tcaagtaata ttgagaaaaa tatctaatcc aatgaaagtg
     9601 gataaaagcg aaataaatat agttttgtct ggtggtacat taaataaaac tgtatatgct
     9661 caaatgttaa tgaataattt aaaaacagaa aatacagata aaaatttaaa atttatatct
     9721 caagaaaatc agccagtgta tggggggata agacttgcta tggcattagc taataggagg
     9781 gtcaagcatg gatagaaaaa aggtaatagt acaaattttt actggaggtt ttaaggctaa
     9841 agaaataaag tttaatgata taaaatgtaa actactagaa ttattggact gtattgatat
     9901 agaaaaggtt atcatgggat ggagtgtaga tgaagaatta tatgtagaaa ctaaaaaact
     9961 attaaataaa tataatgttg aactatatct ttggttgcca tgtttttctg aaataggatt
    10021 acttgaaaaa agtaatttgc taattgatta tatggggaag gaagtcaagg gctatgcttt
    10081 acaagaggat gaaaattttg agttttattg cccaaataat aaggaaaata taaaaaatat
    10141 aaagaaaata tatagtactt attttagtga tattaaattt gatggaattt ttttagataa
    10201 aataaggtat ggttcttttt cgaataaact aaatggtgtt tttaattgtt tttgtaaaga
    10261 ttgtttaaat atatatgaaa aaaataatat tgataaagag tcgttaatga gagaaatgaa
    10321 taatgtgtct aagggagtta tagataatga agatactcca tttggtataa cagaatacat
    10381 taatggaaaa tacaaattta aaaataaaat atgggaagat ttttttgaaa caaaaaatca
    10441 aaatatatac aaggcattat tagagataag taattatttt aaaagtaagg gattgaaaat
    10501 aggtatagat actttttctc cttttatttc ttactttaca ggtcaagatt tagaaaaatt
    10561 acaagaagta gcagatttta taaaaccaat gatgtataga actacaaaag ctccagctgg
    10621 tttaccattt gaatttgaaa ggtttattga tgaatccata cataagaata aagagattgg
    10681 aaaaagaaat ttcttagata ttttaggaat taaggagtat gaaggagatg tatatcctat
    10741 agaatttgta ctagaggaaa tcgattttat gacaagaaat cttaataaag atatatatgt
    10801 aggagttgaa ataaataaaa aggaaattat atctcctgtt acacctaagt acataagtga
    10861 aaatatttca aatataaata agacaacagt aaaaggatat gttttatctt gggatttgtt
    10921 gtcagcacca aaagaaaata ttgatgaaat tataaaatgg tttcggaatt agtgaggtga
    10981 taaagtgagt aagatattgg aaatgcaaca aatatataaa agttttggaa aggtagatgt
    11041 tttaaaaggg ataaattttg agttagaaaa gggagaagta agagctcttt tgggagctaa
    11101 tggagcagga aaatctacac ttattaaaat tttaggagga gttcattctc aaactaaagg
    11161 taaagtaatt ttgactggta atgaaataat gtttaaaaat ccacatcatt ctaaagaaaa
    11221 tggtattagt ataatttatc aagaattaag cttgatacca acgcttacag tacttgaaaa
    11281 tatttttctt ggtagagaaa ttttaaaagg aagtgtgttt ttaaacaaga aagacatgct
    11341 tgaggaatgt atgaaaattt gtgaggattt tgattttaat atagacccac atatggtagt
    11401 ttctaattta agtgtagcaa accaacaaat ggttgagata atgaaagctg tttcatgtaa
    11461 ttctgaaatt atcataatgg atgaacctac aacatcactt actaataatg aaaaagatgg
    11521 gctatttaaa ataataagta gactaaagtc taaaggtaaa acaatagtct atatttctca
    11581 catattagag gaagttttta aagtatgtga tagtgcaagt ataatgaaaa atggaattat
    11641 gattggaact tatgatgtag aggatttaac taaattaaag ataactcaac ttatgacagg
    11701 aaatgataat cacaaactta ttgagaagaa ggattataca tttgcaaaat atgaagagcc
    11761 accagtacta gaagtaaaga atataagctg gtctaacaaa ataaaaaatg tttcatttaa
    11821 agtttataga ggcgaagttg taggtcttgc aggtcttgta ggttcaagaa gaactgaaat
    11881 tataaatttg atatttggta tagataataa agatagtgga gacattttta tcaatggtaa
    11941 aaaagtagac ataacttctc ctaaggttgc tataaaaaac aaaataggat tgattcctga
    12001 agatagaaaa aatctaggat taattttaga acaagctata tacacaaact catcatcagt
    12061 acaaattgat aaattcaaga aattcttgtt tatagataga aataaagaaa ttgagttttc
    12121 taaaaaaggt gttaaggaat taggaataaa agttaatgat gtaaagcaag aagtaaaaga
    12181 gcttagtggt ggaaatcaac aaaaagtagt agtttctaag tggttagaga aagatttaga
    12241 tttaataata tatgatgagc cgacaaaggg aatagatatt gctgcaaaag aagatatttt
    12301 caatacaatc aaagaatttt caagtaaagg aataggagta atatttatat cttctgattt
    12361 agaagaagta ataagagtaa gtgatagaat tttagtaact agagaaggtt ctattgtaga
    12421 tgatttaaaa aatgagaata tacaggttca agatataatg aacagtatat ttaatgtata
    12481 aaaaatgaaa atggaggact gaaatgaaga aagcttttga gaagttaaat ttaagagatt
    12541 atggagtatt attaggtttt ttagctttat gtatagctat aagtattgca actccatcat
    12601 ttttaggtaa acaaaatata cttaatcttt tacgacaatc ttctataatt ggaataattt
    12661 cagcaggtat gacttttgtt ataatatctg gaaactttga tatatcagtt ggagcagtag
    12721 cagctcttgc aggtgctata acaatgaaat ttgcgactgg tggaactaat ttgtttgcat
    12781 ccatgctctt aggtgtagcg atttgtgcag taataggatt aatcaatggt atagtagttg
    12841 caaagataaa tgtaccatca ttaatagcca ctatggctat ggtcactatt gtaagaggat
    12901 tgctgttgat gcttacagga ggatatccaa ttactgaaaa tataccaata cttgattaca
    12961 ttggaaatgg atatttgttt aatataccaa taccagtagt aattttcttt atagtggtgt
    13021 tagcttcatt tattgtactt aataaaacta agtttggtag atatgtttat tctgtaggag
    13081 ggaatcaaga agcaagtaaa cttaatggta taaatgtaga ttctcacaaa gtgaaagtat
    13141 ttattattaa tgctgtactt gcaggaatag ctggaatcgt actaacagga agacttggga
    13201 ctgcaactgc gattgcaggt gaaggatatg atatggatgc aattgcatct gtagtaatag
    13261 gaggtacatc agtggcagga ggttctggtt ctgtacttaa aactgttata ggagtattac
    13321 ttatgagtgt tataaacaac agttttaatc ttcttggaat agatgtatat ttccagtaca
    13381 tatttaaagg attaataatt ttagcagcag ttggatttga ttcttatagc aagaaaaaac
    13441 ttgcttcaag ataaaaaata agggggattt ttttatgaaa aaattaatga aacatttagc
    13501 attgttgcta tcacttgtta tgatatttgg tttagttggt tgttcaaatg gtggtgacaa
    13561 agaaaaaaat ggagaaaaga aaatagcagt attactacca ggttcaacag gttactttgt
    13621 agcaactaaa caaggtatag atgcaaaatc taaagaatta ggtgtaagcg tggaatatgc
    13681 agatgctcaa tgggatgcta gtaaacaatt atcacaagct gaagatttta tggctaaagg
    13741 ggtagacatg attgcattat gtggagttga ttctgctgta agtgagagaa tagttaaagc
    13801 tgctaatgat tcagacgttc ctatagtagc atttacaaat tctataggaa gtaacccaac
    13861 tggagaattt aaaggtttag ttacatatat aggacaaaat gaggaagaaa caggagcatt
    13921 aactggaaaa attgctaaaa acttattagg agaaactggt gggaaagctg tattaataga
    13981 aggagtacca ggaactactc cacaagtatt aagaaaaaaa ggtcttgaaa aagaattaaa
    14041 agatagtaat atagaaatag tttataatca aacttcaaat tgggagaaag agcaagcttt
    14101 aaaggtaaca gaagatttaa ttcaaaagaa aactgatttt aatgttataa tatgtcaaga
    14161 tgataattct gctacaggtg caggtcaagc tttaaaagat gctggtctta aggataaagt
    14221 taaagtcata ggtttgggtg gaagtaaaga tggattaaaa gctataactg atgggttaat
    14281 agatggtaca acttatatgt ctgctgtaga agaaggtgga ttagtcatag aaaaggcatc
    14341 taaattcctt aaaggtgaaa aaatagaacc tgttacacaa attaaacaag ttgaagttaa
    14401 caaagataat atttcagagt tcaagggtga atggtaaatt gtcatagata ataaataaga
    14461 tttttagagt tactttagaa actatataaa gtaactcttt ttttaattta atttagtgat
    14521 aaattattga atagtatttc taaacaaata aaaaattaat ataaacaaag acttaattat
    14581 actaaattat aagtaaaact aatataaact tatggaaaaa aggaaaacgt ctaaagtatt
    14641 ggaattacta tagtgtaggt gtggtattaa aaaaacataa aaaaacataa aaaaattgtt
    14701 gaaaaaaata tagaaatttt gtataattaa agatttgaaa agtgcggtat acaaagccaa
    14761 ttcttttgta tataatatat ataaattcag aaaaaggaag ggatatgtat ggccaggcgg
    14821 ttatttttag aaaggattgg acttatattt ttaggttcag caatattagc atttggagtt
    14881 tataattttt attatttaaa caacataaca gaaggtggag tgttaggtat tttactactg
    14941 ttaaaaaatt tattcaatat acaaccagca atagcaaatg ttgtaataga tggtttgcta
    15001 cttttagtag gatataaatt tttcggtaaa aaattcctta tatattcaat agtagcttct
    15061 ataacttttt cagtgttata tgacttattt gaagctatag gacctctagt accacaatca
    15121 caaaatatgc ttttatcaac tattttggca ggtgtaactg ttggaactgg tgtaggtata
    15181 gtagtaaaag caggttgtgc atcaggagga gacgatgctt tagcattagt aatttctaag
    15241 actacatctt tgaacatagg gcaagtttat ttagcaacag atgttattgt attgttgtta
    15301 tcattatttt atctatcagc gtttgatatc ttttattctt tgattgctgt tacaattagt
    15361 ggcaaggtaa ttgattttat ttattatcat ggtaaaagtt tggatatgga tataagtaat
    15421 gatatagttc cagaatgtta atataaatta aataacttta gattttatta ggggtggcag
    15481 atttgccacc ttttttgtgt ctaaatcttt atagaaagta taatcctatc taggtttttg
    15541 caaatctcaa atatagaatt atgttgaaat gactgggaat acgattgcta gatgtgcatt
    15601 ttaaaaaccc aattttatat ttatgaaata ctaatcatta atggatgttt cttaatccta
    15661 tctttatacc taaaatgata agataaatga agtagcaata caaaactaaa atttgtaaaa
    15721 gagattaaat taaatggagg agctaaaatg ttggatatag ttatgatagc attgttattc
    15781 ataggatttg tttccatgaa gttacttgct gattggtgtg ggaaacaaac tgaaaaaaaa
    15841 taatgtaatg tatggaggga ttacctatgt ggtttctggc agttgtaatt ataatgttaa
    15901 taatatattt ggtatatgcg cttattaatc ctgaaaaatt ttaaacagta taaggctaat
    15961 aagtaagttg aattgggggg aacaattttt atggtgcaga tagtaatagt gttagcgatt
    16021 tttatgattt tagtaatacc tatgggcaag tatttatatc atatagctac taatcaaaaa
    16081 acatttggag atagactatt tgataaagtg gacaatttta tatataaggt atgtagtata
    16141 gataagaaaa aagaaatgaa ctggaaacag tatgcattag ctttattatt tacaaatgca
    16201 gttatggtat ttataggata tatcatactt agaactcaaa gtatgcatat ttttaatcca
    16261 agtggaatta aaagtatgga acaaggacta tcatttaata caataataag ttttatgaca
    16321 aatacaaatc ttcaacatta ttcaggtgaa tcaggacttt catactttag tcagatgaca
    16381 gtgataatat atatgatgtt tacatcagct gcaacaggat atgcggctgc tatggcattt
    16441 gtaagaggtc tagtcggtaa aaagaaaaca ttgggaaatt tttatgtaga tttaattagg
    16501 ataacaacaa gagtactttt accaggagct ttaattattg ggcttatatt agttacacaa
    16561 ggagtaccac aaacttttgc tgggacagaa acagtgacta ctatagaagg aaagcttcaa
    16621 gatatagcta gaggtcctgt ggcagctctt gaatcaataa aacatttagg aacaaatggt
    16681 ggtggattct ttggttcaaa ttcatcacac ccatttgaaa atccaacaat aatatcaaat
    16741 atagtagaaa tattatctat gatgatatta ccaggagctt gtgttgttgc atttggtcac
    16801 atgataaaga ataaaaaaca aggttgggtt gtgtttggcg caatgtcaat catattttta
    16861 ataggtctag tagtgtgctt taaagcagaa agtgcaggaa atcctatttt atcacaatta
    16921 gggcttaatc aaagtatggg aagtatggaa ggtaaggaag ttagatttgg tatagcacaa
    16981 tcatcattat tcacaacagt gactacttcc tttacgacag gtacagttaa taatatgcat
    17041 gatacactga ctcctttagg aggattagta ccacttttaa atatgatgct aaatgtagta
    17101 tttggtggta aaggcgtagg tctgatgaat atgttgatgt acgctataat agcagtattc
    17161 ttatgtggat taatggtagg aagaacacca gagtttttaa ctaagaaaat cgaaggaaaa
    17221 gaaatgaagc ttattgcttt acttattata ttacatccac ttttaatact aatgttctct
    17281 ggtctttcag tagcaatacc agcaggactt gaaggaattt caaatcctgg attccatgga
    17341 ttatcacagg tattgtatga gtttgcatct tcagcagcca ataatggttc aggatttgaa
    17401 ggtctaggag ataacactat gttttggaat ataacaacag gtatagttat gttctttgga
    17461 agatatgtgt ctattatagt actactagca atatcaagtt tgttagcatc taaaaaagct
    17521 gtaaatgaaa gtataggaac acttagaacg gataatttta catttacaat agttttagta
    17581 cttgttgttt taattgttgg tgctttaaca tttttcccag ccttagcgct tggacctatt
    17641 tcggagcatc tagtgttgtg gcactaaaaa attgggaggg agtaaagttt tatggaaact
    17701 aaaaaaacaa agtttattac aaaagatatt ttcaaaactt ccattataga agcttttaaa
    17761 aaattaaatc ctaaatatat gatgaaaaat ccagttatgt ttgttgtaga aattggattt
    17821 atagtaacat tgatacttac aatagtacct agtttgtttg gtgatgaagg caataactta
    17881 agagcttata atgggattgt aacaataatc ttatttataa cagtcctttt tgcaaatttt
    17941 gctgaagctg tagctgaagg aagaggtaaa gctcaagcag atacacttaa gaaaactaga
    18001 aaagatacag tagcaagggt tatagatacg ttaggaaatg aaaaaatgat aagtgcaagt
    18061 gagctaaaac ttggtgatat tgttttagta aatgctggag aagttatccc aaatgatgga
    18121 gaagttatag agggtatagc ttctgtggat gagtcagcaa taactggaga atctgcacct
    18181 gtcatgagag aatcaggtgg agatttcgca tctgttacag gtggtactac agttgtaagt
    18241 gactggttaa agataagaat aacaacaaaa ccaggagaat cgttcttaga taaaatgata
    18301 gctcttgtag aaggtgcttc aagacaaaaa acgccaaatg agattgcttt aaatactttg
    18361 ttagtagggt taacacttat atttatggtt gttttaatat ctctttatcc aatggcaaac
    18421 tatgcaggtg taaagattcc aatttctact atgatagctc tcttggtatg tttaatacca
    18481 actactatag gtggattgtt gtcagctatt ggtattgcag gtatggatag agtaactaga
    18541 tttaatgtaa ttgcaatgtc aggaaaagca gtagaagctt gtggagacgt tgatactatg
    18601 attttggata agacaggtac gattacttat gggaatagac ttgcatcaga atttatagca
    18661 gttggaaatg cagattcaaa agatttaata aattattctg ttatgtgttc attaaaagat
    18721 gatacaccag aaggaaaatc tatagttgat ttaggtatga aattaggttc aacaggtaag
    18781 acaaaagagg cagaggaagc tgagtttgta gaattttctg cacaaactag aatgagtggt
    18841 attaacttag ctgatggtag agctataaga aaaggtgctt atgatagcat aataaaaaga
    18901 gtaaaagaat ctggtggtac agtgccagaa gatttagaag aaaatgtaaa tcgagtagct
    18961 aaactaggag ggacaccttt agtagtgtgt gtagataatg agatatatgg aattatctac
    19021 ttaaaagata cagtaaaatc agggcttgta gaaagatttg ctagacttcg agagatagga
    19081 ataaagacaa taatgtgtac aggggataat cccttgacag cagctacaat tgcaagagaa
    19141 gctggagtag atggatttat tgcagaatgt aaaccagaag ataaaataga tgcaataaaa
    19201 aaagaacaat tagagggtaa aatagttgca atgacaggtg atggaacaaa tgatgctcct
    19261 gcacttgctc aggcagatgt aggtcttgca atgaatagtg gtacaacatc tgcaaaagaa
    19321 gcagcaaata tggtggattt agattcagac ccaactaaga tattggaagt tgttgaaata
    19381 ggaaaacagc ttttaataac tcgtggggct ttgacaacat ttagtatagc aaatgatgtt
    19441 gcaaaatatt ttgcgattat accagctata tttactcttg caatacctca aatggagata
    19501 ttaaatgtaa tgaaactttc tacgtcattt agtgcgatat tatcagcttt aatatttaat
    19561 gctataatta tcccatgttt aatacctctt gcaatgaagg gagtaaaata tagacctatg
    19621 aaatcacaag ctatgttaat gagaaatatg cttatatttg gtcttggtgg agttatagta
    19681 ccatttttag gaataaaatt aattgattta ttgattacac ctttagtaat ggttcttaat
    19741 ttaggttaat aaatggagga tataaaaatg aaaacattaa agccagcatt acttgtaagt
    19801 attgtattgt tagttgtttg tggacttgta tatccacttg ttttgacagg tgtaagccaa
    19861 gttgcattta aagataaagc taatggaagt atgatagaag taaatggtgt aaaagtaggt
    19921 tcagaactta ttggtcaaag ctttacagat gcgagattct ttaagggaag agtatcatca
    19981 gtaaactata atacttatac aaaagaagat ttagtaccag ataaagatgg aaacacatct
    20041 tatggaggtg tatcatcagg gtcttttaat tatggagcta caaatccaga acttcatgat
    20101 agagtacaaa aagatataga acagttttta aaggataatc caactgtcaa aagagaggat
    20161 ataccaacag atttacttac agcatcaggt tcaggacttg accctaatat aagtccagcc
    20221 tctgcaaaaa ttcagatacc agctatagca aaagcgtctg gaatatcaga aagtaaatta
    20281 caaaaaattg tagaagataa tacttccaaa aaactttttg gagttttagg tgaagataga
    20341 gttaatgtat taaaggttaa tgtagaggtc gcaaaaatat taggtcttat atagtatttt
    20401 gtggaatttt agcaacaaaa tattactcaa gatttaattt aaaggttaaa ttttaaagag
    20461 tgaaaatcga taatgatttt cactttttta tttgaatatt actaatattt ggaataaagt
    20521 gattgtaagt ataaaatcca aaaaaaggtt gcattatcaa aatgataata ctataatata
    20581 tttatcgtta tcaatttgat aatagatttg gacatatagg aggaaatagt atgttatata
    20641 ataacgcatt agagttgata ggaaacacac ctgtagtaag gctgaataat ttaggctgta
    20701 ctaatgggtg ttctaacata tatgtaaaat tagaaaaagt aaatccagca ggaagtataa
    20761 aagatagagc tgtttatgaa atgttagagg gtttagagca aagaggagaa ttaaaaaagg
    20821 gagatgtact agttgaagca actagtggaa atactggtat tgcattatct atgataggaa
    20881 aattaaaagg atatgaagta gtaatcgtaa tgcccgaaac aatgagcgtt gagagaagag
    20941 atttaatgaa ggcttatgga gcaaatttgg tgcttacaga tggtaaagct ggaatggcag
    21001 gttccataga aaaagcaaat gaacttttaa aagaaaatcc aaactataaa agcttaaaac
    21061 aatttgaaaa taaagataat ccaaatgcac attataaaac aacagcagtt gaaattatga
    21121 atgatgtcaa agatttagat atattcgtat gtggagttgg aacaggtggt actttaatag
    21181 gaactgcaag atatttaaaa gaacaaaatc caaatataag agtaattgca cttgaaccaa
    21241 aaaaatctcc agctatatca gaaaacaaga ctggacctca taaaatacaa ggaataggtg
    21301 ctaactttgt tcctgaaaat tatgattcta gtgtagttga tgaagtaata ttagtagatg
    21361 atgaagatgc atttgataca gttaaattac ttgcagcaaa agagggaatt ttagtaggga
    21421 tatcttctgg agcaaatata tttggtgcta tagagatggc aaaaaagtat ccagataaaa
    21481 agatagttac agtagctcca gatggagttg ataagtatat gtctatggga atattttaat
    21541 aattttattt gagggagtga atactttgtt taagaaaata aataaagata ttgaatatat
    21601 aatgaagaat gatccagcag ctaggtctaa gatagaagtt tttttacttt atccatcagt
    21661 tcatgcaatg ataatgcata ggatggctca tgctctttat aagaagaaga agctttttac
    21721 agcaagatta atatcccaga tatccagatt tatgactggc atagaaatac atcctggtgc
    21781 taaaatgggg gaaggtatat tgatagacca tggaatgggt gttgtaattg gagagacagc
    21841 tgaagtaggt aatagggtga caatatatca aggagctact ttaggagcta caggaaaaga
    21901 tactggaaag agacatccaa ctgttggtga tgatgtatta attggtgctg gaacaaaaat
    21961 actaggacct ttaaatatag gttcaaattc aaaaattggt gcaaattctg ttgtcgttaa
    22021 ggatgtacct aatggtgcaa cagttgttgg tataccagca aaaatagtaa aaataagaaa
    22081 cctagaacct gtaaaaaaga ataaaaaaga agtgtcctat gagtatgatg aattagataa
    22141 tgtatattat atataggatt tatataacta taaaataaac tttataaaaa ttattttaaa
    22201 aggtattttt ttcaaatttg aggacataat aaaaaagaac tgaatgttac agttttggtt
    22261 catcaatctt tgataaacaa gattttaaat ttgtagcgtc taggaggatt ttattatgaa
    22321 agcaaatgtt aatcaagata cttgtatagg ttgtggatta tgtccatcaa tatgtccaga
    22381 agtatttgat atgaaggatg atgggaaatc acatgttata gttgatgaag taccaagtga
    22441 tgctgaagaa tcagcagcag aagctagaga aagctgtcca gttgatgcaa tagatgtgca
    22501 ttaataaaat aaatgcctat aaaaattgaa ttttcaattt ttataggcat ttgtctttta
    22561 ttcagttagt atttcaacac ctgtttctgt aactaaaacc atacattccc attgagcaga
    22621 taatttacca tcttttgtat aaatagtcca attattttct tcatcaacaa aaatttcgtg
    22681 agttccttca tttaccatag gctctattgt gaatatcatc ccaggaacca atagcatctc
    22741 agtccctttt ttagatacat aacttacaaa agggtcttca tgaaagctta atccaactcc
    22801 atgaccacct atttctctaa caacagagta tccatttttc tttgcataag agttgataac
    22861 atcacctata tctcctaaga atccccatgg tttggcaact tcaagacctt tttggacaca
    22921 ttcctttgta acttgaacta atttttttgc attttcgtta acattaccta tcataaacat
    22981 tcttgaagca tctgaatagt atccgttaaa aatagtagaa acatctacat ttataatatc
    23041 tccatctttt aagataatgt ctttacttgg aataccatga caaacttcat tatttactga
    23101 tgtacataca cttttaggaa atccattata gtctagtgga gcagggatag ctccattttg
    23161 tagtgtaaag tcatgaacca acttatttat atcttctgta gacattccaa tttcgatttt
    23221 atctgaaata taatccaaaa tttttgtgtt tattacagca ctttttttta tttcctcaat
    23281 ttgactttca ttttttataa tatcatgagt tggtacaata tgacctaact tagcaaatga
    23341 atctatttta ttatccatat ccatatgaca ttttttatat tttttagtac ttccacacca
    23401 acagctatca tttctattta acttcataac ttttcatcct tcttttatta tttatttaaa
    23461 ttatgggtta cataaactat tttaaaacat aaccattata aatattataa cataaatact
    23521 aagaaatcct ttatagatag tgatttattc aaagtataaa ttattatatg gttattgttg
    23581 atgaaaagag gatagttaaa tgtattattg gatattagcg cacttaatag tttagttcaa
    23641 gtttttcaag aaaatcatat gttagaggac aattttcctt taaagtattt tttgaatctt
    23701 catcaagata ataatgtaca aatgattctg caaaaaattc ttctataaac ttgcttatat
    23761 atgcatgtgc aacttgctta gggtcataaa gcttatatct ttcctgagca aatacttcca
    23821 taaattcttc agaatttgat tttttattta aaacattttt atctatagca tgtgcagttt
    23881 catgtaattc taaatttttt gatgaatgta catttgcata gctagggtta ctataaccta
    23941 ttttagccac tatagaatta tttctatata taccaggtac atctttccaa gtcttgcctg
    24001 atcttaccca acatggaggt aattgatatt ttaaatcact aaattctggt tcatcagtta
    24061 gatttgaatt tattaatttt atttttatat ccttttcttt aagtgtctgt aaatatttaa
    24121 tatttatttt tgcaagtctg tttttcatat tttcaacttc atctttatca taattcaagt
    24181 catcaggaag tataagcatt tgttcaatta tatctatact actgtctttt atattttcag
    24241 attcttcatc aaatttatat tgaagagtat tattttcata gatagttata tttgtattat
    24301 tttctataga ataagagtga atatcctcta aatctaaaat attcatttgt tgtaaaatat
    24361 gttttgtttc gccactccac actactaaag aaagtgaatt aaaattaatt agtggatttt
    24421 cttttgcttt tatatcattg cttatatcta catcaaatac atattcaatt gagtcttcta
    24481 tgttaagact atctgtattc tttttaatat ataaatacgc ttgttttaag tctatataaa
    24541 tctcatctaa agattcgtca taaaattctg ataaattatt agtaaagagt tcttcactta
    24601 aaagttcatt tttatcagtt atactttcaa ttaaaatatt aatttctttt atcatattgt
    24661 aaatacttat ttttctattt gctttttcac tagatacatt gcatggcgca agagcaatgc
    24721 aataaaaatc tttttctttt ttataaaagg ctttaagtga ttttgataaa agtgtaaagt
    24781 ctacacctat ctgtaagttg accttaagtg agtcattttg taataattca gtattatttc
    24841 ttttagctgt atttaaattg ttttccataa tcattgcccc ctttttatta gggtatcata
    24901 atttttttat atgtgtcaat ttgttttttt gaaaattata atttttttga aaattttatt
    24961 ttttataatt tgtgaatttt ttattataga aataaatatg ttattattat gtcaaaattt
    25021 agcattagat tcatgtagag tatgttttat acatatatta taattaattc tttgaagatt
    25081 gcatattcaa aatacacacc atttttttta attctataat ataatattta tatatttaat
    25141 gttaggatgt gatttaaggt gtattttaca tatattataa gatgtaaaga tgattctcta
    25201 tacactggat ataccagtaa tatagttaga agaatgaatg aacataaatt aggaattaat
    25261 tcaaaataca caagagcaaa gggatttgaa aaactagaag tttattttgt gaccaacaca
    25321 aaaagcaatg ctatgaagtt agaatattat ataaaaaaat taactagaaa taaaaaattg
    25381 tctataataa aaaacccaag tatacttata aatttaattg ataataaaga agattatatt
    25441 attggtaagg aaatagaaca attaacataa atatttatag taacattata atgggtgcat
    25501 atggtgtaaa aacttgacaa taaatactat tgtaatataa tgaagttata aatatagtaa
    25561 attaaaatag ctaggggagc caagagctga gaggtatacc gacccttaca cctgatctgg
    25621 ataataccag cgtaggaaag cttagtaaaa attcaagtat gtattatttg tgatatatgc
    25681 tatttctaag ctatacctat tgtaggtata gcttttttaa ttaagttatt taaattaaac
    25741 tttaggaggc aacaatgagt aattataaaa taccaacact tacaatagcc ggctctgatt
    25801 catctggagg agctgggatt caggctgatt tgaaaacgtt tagtgcaatt ggtacttatg
    25861 gtatgagtgt aataacagct ataacagcac agaatactaa aggagttttt gctgtagagg
    25921 atttaaataa gaaaataata aaaaaacaaa tagaggcagt ctttgaagat atacctccaa
    25981 gagctgtaaa aataggaatg gtttcaagtc ctgagattat acttgaaata gttgagaatc
    26041 taaagaaata caatccaaaa tatttggttg tggacccagt aatgatatct aaaagtggat
    26101 attatctatt aaagccagag gcaaaagaaa acttaattaa gtatttgata ccacttgcat
    26161 acataattac accaaatata ccagaggcag aagaaataac aggaataaaa atacataatg
    26221 tagatgatat gaagagagta ggagaagaaa tactacaact tggacctaaa tttgtactta
    26281 tgaaaggggg tcatttagat ggagaagcag tggatatttt agttggtaaa aatatattta
    26341 aagtttataa aagtgaaaga attgataaaa aaaatacaca tggaacaggg tgcacattat
    26401 cttctgcaat aacatcttat ctagcattag gatatgaaat aacagaagca gtaaatctaa
    26461 gtaaaatcta tataacagaa gcaataaaaa gaagttttga tataggtcat ggagtaggac
    26521 cagtacatca tttttataaa tttgagtaat aaaaaaatat tggaggtaga atatgtataa
    26581 tttaattaaa gatgtaaaaa aattaaatcc attggtaatt cattatacta ataatgtaac
    26641 tataaatgat tgtgctaatg taactttagc tgtaggagca agtcctctta tgagtttttc
    26701 atatgaggaa gtagaagaaa tggtaagtgt agcaaactct gtagtaataa atataggaac
    26761 tatgaactca aatatgttgg atttattcct attagctgga aaagcagcaa ataagtacaa
    26821 taaacctgtt gttttggacc cagttggagt atttgcaagc aaagcgagag cagaacttac
    26881 aagtagactt ttaaatgaag tcaaatttag tgttgttaaa gggaatgtat cagaaataaa
    26941 gtttataggt ggctttaatg taaggggtaa aggtgtagat tcatttgatg aagaagaaga
    27001 ttctactgag ataataagaa agattgctga aaagcttgaa tgtgtagttg tagcaactgg
    27061 aaaaatagat ataataacta atggtaaagg tacatataaa ataaacaatg gaactgataa
    27121 attaaaagga attacaggaa ctggatgtat gactgctagc ttgattgcaa gttttatggc
    27181 tgttactgag aatatattag aagcagcaac aatgggtgtt cttacaatgt ctttaagtgg
    27241 ggaattagct aatttaaata atccaccaat aggtacattt aaagaaaatc ttatgaatgc
    27301 tatttatcaa atggatatag atactttaag taaaaattct aatattgaat ttttaaatta
    27361 ataaagagta agggagactt aaaatgattg ataaagaatc tttaaaaaaa tgtttaaagt
    27421 tgtatttagt aactgactct gaaatgctta aaggtagaga cttttataaa tgtcttgaag
    27481 atgcaatatc ttctggaata acaactgtac aacttagaga aaaaaatgct tctggaagag
    27541 agtttttaag aaaagctatg aaacttagag aaataactaa acaatatggt gttaagttta
    27601 taataaatga tagagtggat atagcactta tttgtgatgc agatggagta catgtaggac
    27661 agagtgacat tgatgttaga gaggttagaa agcttatagg aaacaataaa attctaggag
    27721 tatcagcaag aactttagaa gaagctattt gtgcaaaaaa tgatggcgca gactatttag
    27781 gtgtagggtc tatattcaca acatctacta aattagatgc aaaatcagct tcgtttgaaa
    27841 ctgttaaaga aattaaagaa aaagtagaca tgccatttgt actaattgga ggtataaatt
    27901 tagacaatat agataaactt aaatgtttag aaagtgacgg gtatgctata atatcagcta
    27961 tattaaaagc tgaagatatc tctaaagaag tggaaaagtg gacattaaaa atataaaatt
    28021 ggctctggta gtgaaaaaaa tggaaaaatt tgatataatc taagagtaaa atttaagcaa
    28081 ttataaatga taaacttttc tacatatact ttttaatcaa gatatatgta gatttggtta
    28141 aaaaggagct ataaatgatt aggatattgt tagtgtgtgt tggaggcatg tcttctaccc
    28201 tattggtaaa taaaatggag aaggatgcaa aaaaaagaaa tatagactgt aaaatatggg
    28261 cagttggaga aggagatata aaatcggagc ttgataactt tgatatttta cttcttggac
    28321 cacaacttag atttatgctt gatgacgtaa agtctatagt tggagataga gctccagtat
    28381 ctataataga tatggtaaac tatggcactt gtaatggtca tgctgtgtta aattcggttt
    28441 tagaaatatt aaaataaata ataaaagtgg attctatata atagagtcca tttttattat
    28501 ttgttattaa taggcttaaa acttagctta ccatttcttt ttttaatctc cacatagcaa
    28561 gtgaaaaact tgaacagcac aatattaaaa atagtaggat aacaggcagt atatctccaa
    28621 taagaatagt ctcaccaaat attaagctac gtaaagcgtt aataacatgg gtaaatggat
    28681 tgagcatcac tgctactttt aatcctccag ataaactttc tatagggaaa agagctgaac
    28741 ttaaaaagaa aataggaagt acaattgcag tcataattgt ttcataaact atttcatttg
    28801 gcaataataa acttatagaa taagctagac ttgataaaaa gaaggctgtc ataaatatca
    28861 gtactatcat aagtaggatt ccaccaatgc cagattctat ccttactgag aagaatatac
    28921 ttactataca caaaatagtt acttctatga aagaaactaa gatagcttct aataattgac
    28981 caaggacgat tgaataccta ctgataggtg caattaagac tctatagaaa cttccactgt
    29041 tcttcattat aaagtttata ataccaccac tactacaaga agaaaacaca accagtacaa
    29101 tgatacctgg tagtataaaa gcagtataat tattgatatt tatattattc atagtttggt
    29161 ttgcaataga actataaagc acaagccaaa gaaaaggttg taatatagta actacaaatg
    29221 agattggatt tttaaaacgc cacttcatac tacgccataa gatatttaac attcccattc
    29281 ctcctttaag ctttttttag aacttgtaag tgctaaaaat acatcttcga gacttggctc
    29341 tacaatttct attgcatcaa attctatatc atgttctaaa agccatttat ttattaaagt
    29401 aaaagcagtt ctactatcgt ttacacttat aaaaattgaa ttttctcgta cgctcataaa
    29461 ctttactaaa ccagtacttt taattgaatc tttatatttt tttatatctt cagtattatg
    29521 aaaagttata cgtagtatat tttgtctaat gtaactacgt aaacttgatg gtgttccttg
    29581 tgctaaatcc tttccattct tcataataca aatattatca ctcaattgtt cagcttcttc
    29641 aagataatga gtagttagaa atatagttgt gccatactca tctctaattt ttaataacat
    29701 atcccacata gattttcttg aatcgacatc cataccaaca gttggttcat ctaaaaatag
    29761 gattttagga gatgatacca tattcatagc tatgtccaat ctacgtttta caccccctga
    29821 atatgatgaa gtaggatatt ttaagtaact agataagtca aacttatcaa ttaaagagtc
    29881 tattctttgt tttgcaatct gtggttctac tttatacatt ttactttgaa ataccatatt
    29941 ttccataagg gataaatgtt catcaataga gatttgctgt gctacacaag caatttgagt
    30001 acgtatccaa gaaggattat ctactaaatc ttttccaaac attgttacat ttccagatgt
    30061 aggtttgtaa aaggtagtca aaatatttat aagagaagat ttaccagcac cattaggacc
    30121 aagtaaagaa aaaatttctc cagcatttac attaaagctt agaccattta aagctttcac
    30181 accattttta tattctttta ctaaattgtc tatttcaatc gctaacatat attattcctc
    30241 ctctttaagt ggaaatatta tctctgtaat atagttatct gggtttcctt ttatcaacat
    30301 acctggtcct ttaataaata cttctctcca aggagtttgt atttttatat tattttcatg
    30361 aatatattta aatatcattt catacgtttt tggcaaatta ctataagagc ctgtgtgagt
    30421 aaaaaataaa gcttttgttt taggaaattc ttttgtagtt atacccatac gatttggagt
    30481 ttcagcagtt ggaacacata attccatttc tgcaacacca gttttttggt caacatcata
    30541 gtagcaaaag aatggtgcac cgttagactt tccttttata gctttaaata catttggaaa
    30601 atactttgtt gcctcagtca taggtccatt ataacgcatg gtagccactg tgactgattc
    30661 gatatttttg atttcaattt tgcaattcat ttgtttacct cctaatgtgt gttcatattc
    30721 ttactagatt tttaagcatt aatacatata agattttgat tagttgaaaa catataatct
    30781 tatctcgttt gcttgttttc aagtctaaca taacataaaa tatatatcct ctcatttgat
    30841 gctccatttt gtaaagttat tatttaagct ttactttaga ttttttagca aattaaaata
    30901 aagcaattta taaaataagt atttacaaat taagtgtatt aatcgtataa tacacttaat
    30961 aggatactag ataaagtctg aaataaaggc gtattttttt aaagtaagtg tactatcgta
    31021 ttaatacact agaataggag gaatcatgaa ttttgaatta gataatacaa ctcccattta
    31081 tttgcaaata gtaaagtata taaagagaca aatagtaaca ggagagttga aaccaggaga
    31141 gacaatacca tcaagaagag aaatggcact taatctaaaa gtaaacttaa atacagttca
    31201 aagagcatat aaggagatgg gtgatatgaa tataataaat acttttaaaa attatcaaag
    31261 cagtgtaaca gttgatgaaa atatattaaa aaatttaaaa ttagagctta taaatgaatc
    31321 actatctgtt tttatagaag atatgaaagc aataaatgtt agcaaagaag aagttttaaa
    31381 aataatagaa gataagtatt agttttttaa caaatagaaa acttagttta gaaagtttat
    31441 ttataagcat attggatgtg gaggatagta aatatgatag aaataaagaa tgtcagcaaa
    31501 acatataaaa ggatgcaagg gcttaaaata agaaagatag aagctttaaa aaatgtaagt
    31561 tttaatatag aaaagggaaa aataactgca ttacttggta taaatggtgt tggtaaatca
    31621 actatgttaa aagcaatagc aggacttata aacattgata gtggagaaat tcgtatagat
    31681 ggagaaaaaa taaatgaaaa agtatacaat aaattggctt ttgttccaga tgtacaatct
    31741 cactttagca atacaacaat aaaagaaaca tttgaattta tggaaatttt ttattcaaag
    31801 tggaataaag aaaaatcaaa agaaatgatg gacatattta aattggatga agatgaaata
    31861 attgataact tgtcaaaagg aaacattgca agagtaaaat taatacttgg attttgccaa
    31921 gacccagagt acatacttct tgatgaacca tttacaggaa ttgatttgtt taagagggaa
    31981 gagtttatag gagtaatagc gcaatatatg gaggaaaatc aagccattat cataactact
    32041 catgagatag tagagataga aagtttagtt gatgaggttg taattcttga tgaagggcag
    32101 attataactt catttaatgc agaagaatta agagaaagag aaggtaaatc tatattagat
    32161 aagatgaggg aggtatataa gaatgaataa acttctatat ctaataaatg tagatttgag
    32221 aagaagcaat aagttttata tagcttatat atcatttttt tcattaatta cacttagttt
    32281 aaatttattc gaaattaata aatttaaaaa ttctaaattt attatgaact tagcaataga
    32341 agattttgga ggtatatttt atggactagg gatacttagt aatcttggtt ttatccaaag
    32401 tatattgttc tttgggatat tgggactttt tatatactat gtttttatgt ggaaaagaga
    32461 gttcttttca aataatcaaa gtatttatac acttatgatg ttacctcaaa ataagtttaa
    32521 gatttgtata tctaaatcaa tagccttgat aacaatgata tatggttttt tgataagtca
    32581 gacagcaaca ttatttatat gtaaatatat atttaatttc atttttagaa atatgcctat
    32641 aatcaatatg aactttgcca aagatttata ttatttaaac ttgaagagta taccaattga
    32701 ttttatctct tttatggcta tttatgtatt tttactactt atagctattt ctattgtaaa
    32761 ttgctgtata ttatttatct tttcctatat aaataaattt tatgtaattt taatcacttt
    32821 gctgattata gcaggcttat atttttcaac aatatatata
//
LOCUS       DAMKMI010000029        32488 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMKMI010000029 DAMKMI010000000
VERSION     DAMKMI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.030"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            385..1206
                     /gene="yidA"
                     /locus_tag="PC429_001302"
     CDS             385..1206
                     /gene="yidA"
                     /locus_tag="PC429_001302"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF3846817.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1343..1492)
                     /locus_tag="PC429_001303"
     CDS             complement(1343..1492)
                     /locus_tag="PC429_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846818.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1499..1984)
                     /locus_tag="PC429_001304"
     CDS             complement(1499..1984)
                     /locus_tag="PC429_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846819.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1997..2749)
                     /locus_tag="PC429_001305"
     CDS             complement(1997..2749)
                     /locus_tag="PC429_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846820.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2926..3555)
                     /locus_tag="PC429_001306"
     CDS             complement(2926..3555)
                     /locus_tag="PC429_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846821.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3516..4079)
                     /locus_tag="PC429_001307"
     CDS             complement(3516..4079)
                     /locus_tag="PC429_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846822.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4089..4313)
                     /locus_tag="PC429_001308"
     CDS             complement(4089..4313)
                     /locus_tag="PC429_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846823.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4318..5409)
                     /locus_tag="PC429_001309"
     CDS             complement(4318..5409)
                     /locus_tag="PC429_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3846824.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5444..5902)
                     /locus_tag="PC429_001310"
     CDS             complement(5444..5902)
                     /locus_tag="PC429_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846825.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5895..6980)
                     /locus_tag="PC429_001311"
     CDS             complement(5895..6980)
                     /locus_tag="PC429_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF3846826.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(7007..7192)
                     /locus_tag="PC429_001312"
     CDS             complement(7007..7192)
                     /locus_tag="PC429_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846827.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            complement(7354..7686)
                     /locus_tag="PC429_001313"
     CDS             complement(7354..7686)
                     /locus_tag="PC429_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF3846828.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            complement(7942..8778)
                     /gene="nadC"
                     /locus_tag="PC429_001314"
     CDS             complement(7942..8778)
                     /gene="nadC"
                     /locus_tag="PC429_001314"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF3846829.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
     gene            complement(8759..10060)
                     /locus_tag="PC429_001315"
     CDS             complement(8759..10060)
                     /locus_tag="PC429_001315"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF3846830.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            complement(10073..10987)
                     /gene="nadA"
                     /locus_tag="PC429_001316"
     CDS             complement(10073..10987)
                     /gene="nadA"
                     /locus_tag="PC429_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF3846831.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            11405..12862
                     /locus_tag="PC429_001317"
     CDS             11405..12862
                     /locus_tag="PC429_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF3846832.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            13085..13513
                     /locus_tag="PC429_001318"
     CDS             13085..13513
                     /locus_tag="PC429_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846833.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            13573..13881
                     /locus_tag="PC429_001319"
     CDS             13573..13881
                     /locus_tag="PC429_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF3846834.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(14028..15050)
                     /locus_tag="PC429_001320"
     CDS             complement(14028..15050)
                     /locus_tag="PC429_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF3846835.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(15225..15731)
                     /locus_tag="PC429_001321"
     CDS             complement(15225..15731)
                     /locus_tag="PC429_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF3846836.1"
                     /translation="MELWDLYNADGIKTRNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            complement(15791..16492)
                     /locus_tag="PC429_001322"
     CDS             complement(15791..16492)
                     /locus_tag="PC429_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846837.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            complement(16710..17780)
                     /gene="buk"
                     /locus_tag="PC429_001323"
     CDS             complement(16710..17780)
                     /gene="buk"
                     /locus_tag="PC429_001323"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF3846838.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            complement(17833..18408)
                     /locus_tag="PC429_001324"
     CDS             complement(17833..18408)
                     /locus_tag="PC429_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF3846839.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            complement(18410..20197)
                     /gene="iorA"
                     /locus_tag="PC429_001325"
     CDS             complement(18410..20197)
                     /gene="iorA"
                     /locus_tag="PC429_001325"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF3846840.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            complement(20258..21517)
                     /locus_tag="PC429_001326"
     CDS             complement(20258..21517)
                     /locus_tag="PC429_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3846841.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            complement(21779..23779)
                     /locus_tag="PC429_001327"
     CDS             complement(21779..23779)
                     /locus_tag="PC429_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3846842.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            complement(23787..25673)
                     /locus_tag="PC429_001328"
     CDS             complement(23787..25673)
                     /locus_tag="PC429_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3846843.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            complement(25700..27541)
                     /locus_tag="PC429_001329"
     CDS             complement(25700..27541)
                     /locus_tag="PC429_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3846844.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            complement(27672..28091)
                     /locus_tag="PC429_001330"
     CDS             complement(27672..28091)
                     /locus_tag="PC429_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF3846845.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            28404..28586
                     /locus_tag="PC429_001331"
     CDS             28404..28586
                     /locus_tag="PC429_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846846.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            28915..29937
                     /locus_tag="PC429_001332"
     CDS             28915..29937
                     /locus_tag="PC429_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF3846847.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(30030..30632)
                     /locus_tag="PC429_001333"
     CDS             complement(30030..30632)
                     /locus_tag="PC429_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF3846848.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(30649..31797)
                     /locus_tag="PC429_001334"
     CDS             complement(30649..31797)
                     /locus_tag="PC429_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF3846849.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSGILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            complement(31790..32167)
                     /locus_tag="PC429_001335"
     CDS             complement(31790..32167)
                     /locus_tag="PC429_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3846850.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
BASE COUNT        10680 a         5562 c         3480 g        12766 t
ORIGIN      
        1 ttataatcat gttgaataat aacatatgat taatggaatt acaatatttt ataacacaaa
       61 actaactaat ataacaaatt accatcagtc tatttattca aacttctaac tatataatat
      121 acatttattt atattaacaa tgcaaacgat accattattt gtaattaaga attttggaat
      181 tattgattac aaagttttta cattctcatc tcacataagt aatattttta tcaaaaaatt
      241 aatattatta aactttctaa attacttatc tcttgatata atagtataca aaacaaacca
      301 tatttcccac tgaaactttg ttcaacacta gctgtgtcaa cgttttcaca ggaaatttga
      361 gtatataatt tattggaggt tgatatgtac aaattaatcg cactagacat tgatggaaca
      421 atattaaata cacaaaaaag gattactcct gaagtctttg agtccattca ggaggcaaaa
      481 agggctgggg caaaagtagt tataacaaca ggcagacctc ttcctggtgt aaaagaactt
      541 ctaaatcaac taaatttaac agatgaaggt gattatgtta tatgctttaa tggagcaatt
      601 attcaagaag ttaaaagtga gaaaatcata catgatgtag aaatgagttt agatgatttt
      661 gattttatat ataataacgt ttgtaaaaaa tataagacta aaatacacat aaatacaatg
      721 acaaatttaa ttacaccaaa tgaaacgcct ggcaaatata cacttcatga agcgaaatta
      781 aataatatag aagtcaaata tatacaaaaa gataaaattg atgaatctat taaaatctgt
      841 aaaattatga tagttgatga accagaaaga ttggaagaaa tcatacagca actacctaaa
      901 aatttattca ataaatatac tatagtaagg tctgctcctt tttatctaga atttttaggt
      961 aaaactacta ataaaggaac tgctcttaaa acattatgta cgaatctaaa tatacctata
     1021 gaaaatgcta ttgctgttgg cgatgaagaa aatgaccaac atatgataaa gtatgcaggt
     1081 cttggtgtgg ctatggggaa tgcacgtaat agtataaagg aaattgcaga ttatgttact
     1141 gatactaata atgaaaatgg agttgcaaag gttataaata aatatatact taataaggct
     1201 atctaatcat ctaaaaaaaa ctaaaaatat acctttagat ttaaatactt ttcacatccc
     1261 tactctatac taatttatat agtatggata ttacctatca tttttatcta aaggtcattt
     1321 ttagtttttc atatatgtat atctatgata gtaccttttg tttgagttta aatattttat
     1381 atgattctac tattgcctca ttaatatcta aacaatatat atcttcatca ttagataatc
     1441 ccgcttcgtt tgtccaagca tcaaaatcac tcttcgtcca gaagaaattc attatatttc
     1501 agcaactagc agcccaattt agatgcttat caagatttat atgaagcact cttaagtttg
     1561 gattattcac atactcaatc ttttcatttt ttataattac ttctatttcc ttacctgtag
     1621 cactacaaat agagtttatt tttatatctt ggttgaatgt gcaagaggtt cctatagcat
     1681 ctatagcaca cattgcactg aaacttctat tatctctcaa agttacttta tgatttgttg
     1741 gcaatgctga tacaggatat ataaaattta tactattccc atcaacaacc attatatttt
     1801 tataaataaa atattgtagt gtcctttcta tatattcttt ttctatattc aactttttac
     1861 ttatatattc agtcacttct tgaagtgtaa cactagaacc tttatctatt atcatatcca
     1921 taatagagat tcttattttc tgttgtgtat tatccaaact attgtatttt tctatttgac
     1981 tcatgtctac tcctctttaa acttcataac actttgataa agtatcagtt aatttgtttt
     2041 tgtactcttc atatttttgg cttcctctaa ttctaggtct tggaatatct atatttatat
     2101 catcaataat tcttccttta tctctcgaca ttactataac tctatcactc atatatacag
     2161 cttcatcaac atcatgagtt accattaatg cagttatttt cttctttatc catattcgtt
     2221 ctagctcttc ctgtaaattt tgtctcattt gaaaatctac agctcctaat ggttcatcca
     2281 ttagtaatac ctctggatta catgctaaag ctcttatcat ggccactctt tgtttcattc
     2341 ctccagataa ttgatgcgga aacagttttt cttttccttt caagtctgac ataaccaaaa
     2401 gttcttctag catttcttct cttttttctt ttggtatttt ctgctgtttc ataggaaata
     2461 gaacattacc tcttatagtt ttccaaggaa ataaagcata attttgaaat ataaaagctc
     2521 tatctatgcc cggtttttta actcttttat catttataaa aacatctcca cgttctgatt
     2581 tttgaaaccc acctattata gtaagtaaag ttgtttttcc acaacctgat ggtcctaaaa
     2641 gacagacaaa ttgtccatca tctatatcca tacttatatc ttctaaaacc tttgtatgta
     2701 ctctattatt tacaaatgtc ttatttatat ctcttacact taacttcatt aatctgcaaa
     2761 cctccatcta caaaggattt tatttataaa ctttaatacc ctatctatta taaatcctat
     2821 taaagctgca aatatcataa gcccaattaa tacatctgta tgcattaatg tttgagcttg
     2881 taccattgag taaccgagac cggcactcgt agctataaac tcggctcaga taacagacat
     2941 ccatcctgta cttatagcaa tccttgaacc agttagtata tctggaagag atgcgggaac
     3001 aattacattt gtaaatatca caattggcga tgctcccata cttctagctg cattataata
     3061 atcctttgat attgctctta ctccttgaat agtatttaaa attataggga aaacccctga
     3121 aaaagctatt aaaaatattg ttggaccatc tcctatacca aaccatacta tagtaagagg
     3181 aacccatgcc attattggaa cttgccttac agaatctact actggagata aaaccctttc
     3241 aaaagtttta gaaaatccca taataaatcc tattggaagc ccaaacaata atgcatacat
     3301 aaaaccttta agtactctct gcaatgttat aaaaagatta gtgactgtct ctgcatctgt
     3361 cacacagaaa aagaaacctt ttattacatc tactggcatt ggaagtaaca aagaactccc
     3421 tatatctttt gctgcaaaat gccagactaa aaatataata actaatagta ttattttata
     3481 taaaatatcc atactaccat cagttttttt cttagttaat ctatcgcttt tatctttaga
     3541 aattttaatt tccaattgct tatttcctcc atatctatat ttcttatatt cctgtactct
     3601 tttaatgctt tcttaaatgt agctttattc tttaaagcat caacactttt attgtaaagt
     3661 gttataaaat cactatatgc tttacttttt ttaaactctt tacttacaac taatacatag
     3721 gtatcataat ctcctaattt agttgtagat tttcttttgc cctctaaccc aattgacttt
     3781 atgcaatcta ttacaacacc atctactaaa ttactttcta atccatatgc tagtgcagtc
     3841 cctaataagg gagctacttc cacattcttg tagtattttt taaccaattc tgactggtaa
     3901 tctctatttt gaatgactcc tatcttttca ggattattat cacttaatag aaatatatca
     3961 gaattttgaa ctacagtacc aacaatttca aatccactat ttacatttac atattgtttt
     4021 gcagcatctt tacatatgat tgctatatct atttcctctg aactcagagc ccactgcatt
     4081 gtacttgctc agcaatcatt aattgtaaaa atatccatta tatcacttac ttttatattt
     4141 tcaaagtagt ctctatttac catataattt attacaaaac cagaggtatc atcagatacc
     4201 cctattctta attttccttt tatttcttct ttatcttcaa aattaaaaaa tattataaat
     4261 atacttccca aaataatgat aaaaagtatt attatatttt tcatattctc catttattta
     4321 taataccttt gctttccagt cttcataact cataccttca ggaaactctg ggttaacttt
     4381 ttcttttata aatttgtcaa aatcatcagc tccacaagag tccaataagc ttgtatcaat
     4441 cgtatcttca tatttaggta tatcatctaa agcctttaaa tctttataca tttgtaaatt
     4501 atttttatat tcttcacctg taactttcca agtaagagtt ctaccttctc caacagtctt
     4561 cttatagata gttctaagtg caacttcttc ttctacatta taatattttg caaatatttt
     4621 agctgcttca gctggatttg tatatatata ttctattgct tttgtatgtg ctagaaccaa
     4681 tttttttgct aaatctggat gttcttttat aaagtttttg tttaatgaga atgaacaaca
     4741 gttatattct ttgccattag ctttttcttt atattgagta gatgctatta tttttcctgc
     4801 accgtcattt tcagctacag aaccccaagg gtcacaagat gtaaaagctt gaattttccc
     4861 agttttaaga gctaaatatt tatctttgtc tgaatccata tttacaactt catattttga
     4921 tgggtcagtt ggtagacctg tttctggacc ataatcactt gtccataaga aatcactcat
     4981 atctgttgca atttttttac ctattaaatc tttaggttct tttatatctt ttgaaactat
     5041 taaatattca gaaccaccag tatggttgtt agctgctatt gttattggtg aacccttagg
     5101 tattgctcca actaacccct tagttcctat gtaacctgca tccatttttc ctgctgccat
     5161 agcttcagga actttaccat ttccaacagt ttttacatta agacctaaat ctttatatat
     5221 tcctgccgat tctgctactg gtcccgcagt catatggtca caattataat aacctactgt
     5281 tattgtataa tcatcttttc cagtttttgc ttcttctttt tcttttggtt tactactaca
     5341 tcctgttaat atagatgtac ccattacacc aaataagatt aatgcttgaa tcttcttaga
     5401 taatttcata taaatttccc acctttatgt attattttta tttttagcaa catttgtcca
     5461 tatcttcgcc aaataaatca acaaattttt taccctctac tctgtaaata gcatatccta
     5521 cattaccatt tttagaccct gatacaatta cttctattgg tgctttatca tcaatatttt
     5581 taaaggttat agtttgattc tctataggtg ctggtattgg ctttgttata tagtggctat
     5641 ttttatcaga aactacgact accatttcat ttgaattatt gcttttttta tatataacta
     5701 ctaaatcttt tttcccatca ttatttaagt cttcttcagc acaagttatt atgtcttctt
     5761 tataattttt tctaaaatat tctaatgttt ctttattcac atttggaata gaagcatctt
     5821 ctttgatgtt atcatcaact atttttgata aaatgattaa cacaactact atgcaggata
     5881 ttataactat ctttttaagc atgatttcct atgacctctt tcatctttgg tattattgat
     5941 ttaaatgcaa ataaaataat taaaatagta catacatact caaaactatc aggtatattt
     6001 aacattagtt tatttgccaa ctgtattgaa gaatttgttg catctaaatt tactgagtta
     6061 ttattcacta aaagtaaatt tgttatatat ccaattatta ttgaagacag tgtcaaagtt
     6121 acagaatata taataacagt tctttttcca ataagtttaa acatacttat aagttctggt
     6181 agatttgtag ctgctccagc tattagaaat gttatcgcta taccaggaga tgctccactt
     6241 gcaacaaggg ctgctataaa cggtatatgt cctacagcac atacatacat aacacatgca
     6301 agtattgcta tacttccaag agatagcaaa cctggatttc ctaaatattt ttgaattata
     6361 ctatttggaa aaatagttgt tataaatcct gcaaaaacca tacccattac tacatattta
     6421 ctaatagtaa gggctaattc accaaaagac catttaagac catcaatcat attttccata
     6481 aaacttcttt tttcttcttc taattctatt acttcttcct ctgaatcatt atgaagtgat
     6541 aattcttctt ttccaaaaca atttcctaaa aatcctacta aaaatggtac taaaaaacca
     6601 gctattagat aaatgattgt aactttagga cctaaaagac ctagacaaag aactacagct
     6661 attggattta ttattggagt tgatgtcata aaagcaagta ctggtcctaa atatgctcct
     6721 gaataataca tacttattcc aagtggtatt actccacaac tacatattgg taaaaacatt
     6781 cctgagatag tacttttaat taaagaagaa aatttcttat ttccaagttg tttttgaaac
     6841 cttcttggag ttaaaacatt gtgaagtaca cctgccaaga taaaacttat cataagccat
     6901 cctgatgctc cattcagtgt aactatagaa gctactatag tatctttaac tccatttaaa
     6961 acaatttcta atatattcat ttttgatact cctaattttt aaacttttaa ttattgttta
     7021 tagcttctac tattgccctt tcaattgtat ctttatttaa tctatcatat ttctttttct
     7081 gatttattat taaagttcct tttgttataa tcccatattt ttttatatac gaaaaatctt
     7141 tccctgcttg atataatttt atttccaatt tatcagcata cttttctgac atttttttta
     7201 taaacactcc atgttcatca caagatggtc aagtgtttat aaattcaact aaaattttag
     7261 acatttgttc atcccctact ttaaacaatt tatcttttac ttaaaataat ccacagaaaa
     7321 taaatttttc tatggattgt atcctatact aatttataac gttataactt ggtccacacc
     7381 tgcactgcct aaacaagcat ataggtttgg gaaacatcca attgtagctc catcaaccaa
     7441 attattctct atacctcttt ctatagcaca ttggtcacaa gccattatta tcatatcagt
     7501 tttttcatgt aatttactta gtctctcgcc tacaggatta cctttagata aaaataaggt
     7561 attatccata aagaaaaaca tccctagaac gtctactcca tgagttcctt gttcaagttg
     7621 tggtattatc attttgtcta gaattttatg tgtattagta ctactaaaaa tataagctac
     7681 tttcattttt attcccccta tactttttat attttacaaa attttacaga tacaattata
     7741 ttataagttt attcttattt atctgtaaaa tagatattaa ctatattatt aaaagtgtat
     7801 atagtattta tctattttta ttaaattatt gattgatttt tttctatttt tattaatttt
     7861 aatgaataaa aaatagactc cttaaattta taaaacaaat ttaaggagtc tattttaatt
     7921 aagtttattt caatataaaa tttatatatt tctcaaattc ttcatactta tatcgagagc
     7981 tttaacagaa tgtgtaagcg ctcctacaga aacaacatcc acaccaattt ttcctatatc
     8041 ttctatagta tctaaattta catttccgga aaattctaca atggctcttc catttataat
     8101 atctactgct tgttttgcca tttccaaact catattatcc aacattatta tatctgcatt
     8161 ggcttctatt gcttctttta ccatgtctaa agtttccact tccacttcta tctttctaac
     8221 aaaagaagta ttttttctag ctaattcaac tgccttctta acactaccag cagccccaat
     8281 atggttgtct ttaagcaata ttccatcaga taaattaaat ctatgattgc atcctccacc
     8341 tactttgaca gaatacttat ctaatattct aagattaggt atcgtttttc tactatctaa
     8401 taattttgta tttgtacctt caagcttttc aacataccta cttgtaatag tagctatacc
     8461 actcattctc tgtaaataat tcaatgctac acgctctcct acaagtaagt ttctagtact
     8521 acctgttaat acagctatct tttcacgatt ctttactcta tcaccatcat ttttatataa
     8581 ttttacatct acatcaccaa gtatctcaaa aactctctta aatacaccta agccagctat
     8641 tataccatct tgtttgcaaa ttaaatctac agtagattta gaattttcat ccacaataga
     8701 attagttgtt atatcttcac ttggaacatc ttctatcaaa gcatcctgta tcattctatc
     8761 aataatcaaa taattcatcg ccaaaatcct cacttgttct ttcaatttct tttatgacta
     8821 atattttatt ttcttttttt acctgttcta tatctatatc catatctggt acatctataa
     8881 acttaacttt tacattatct ataactgagt ttatattttt agctgctctt tttgaaaata
     8941 caagcccttc aagtaatgaa ttacttgcca atctattggc tccatgaaca cctgtacaac
     9001 taatttcacc aactgcatat aaattttcca tagatgtttt tgaatccaaa tctacatgta
     9061 tcccgcccat aaaataatgt tgtgcaggag atacctttat acattctttc gttatgtctg
     9121 ttcctctagc taaacattca ttataaataa aagaaaatct attaattaaa tattcactat
     9181 ctaaatgtgt tgcatctaaa tacacataag gtaatttatc tttttccatt tgctcaaaaa
     9241 tagcttttga aactacatct ctaggaagaa gttcatccac aaatctttct ttatttttat
     9301 ttaaaagttt agctccctct cctcttaatg attcagatat aagcattctc ctaccttcat
     9361 tattttcttc ataaaaggct gtaggatgta tttgaatata ttctaaattt tctattttta
     9421 tattatttct taaagctatt gcaattccat cacctgtaag atgtctttga ttagtagaat
     9481 ttttaaaaag cccaccaatt ccacctgtag ctaaaactac tatttttgaa aaaacatgaa
     9541 tctctttttt agatttaaaa attctagctc ctatacattt attgccatct tttaatatat
     9601 ctaaaagata tgcatcttct ataagagtta tattttctct agttttagct tcttttaata
     9661 aagttttaaa aacaacttct ccagtattat ccttactatg aactattcga tttacactat
     9721 gtgctccttc tcttgtgtaa tctatctctc cattttcttt gtctaaaggc ataccatatt
     9781 ctactatctg acctacattt tctatagatt catctgcaag tacttgaact gcttcaactc
     9841 tatttttata ctgcccagcc ttcattgtat cttctacaaa ggattctata tcatcaatat
     9901 ttctagcagt tgaaattcca ccttgtgcaa gatatgtatt gttgttttca attgttgatt
     9961 tcgatactac tagcacattt aaacttttat ctaaatttaa tgcacagtat aatcctgaaa
    10021 ctccacttcc aactattaaa acatcttgtt ctaaattcat aagtctatcc ctctatcctt
    10081 ctgctaattt gtgcatgtta agcagtgaat ttaaagcctt ttccatgatt tcatcttcta
    10141 caataacttc attttccata tttttaagtg tactatataa attttctaat gttgtctttt
    10201 tcatatctgt acatagtatt tttccaccag gaatataaaa gtttttatta ggattatttt
    10261 tctttaattc atgtaatata ccttcttcag ttgctattat aaagtctttg tttttactat
    10321 tagtagcata ttttattatt cctgatgtac tacctacata atctcctaaa tctcttattt
    10381 cttttttaca ttctggatgt actaatactt ctgcattttt atacttatct tttaactcaa
    10441 ttatttcttc agctcttata ttgttatggt atttacaata tccgtcccaa aatataaagt
    10501 ttttatctgg aaattgttct gcaatatatc ctcctaaatt tttatctgga aggaatataa
    10561 tttctttatt atctatgttt tctagtattt taattgcact agaagatgta acagaaacat
    10621 cacaatgtgc cttaacttta gctgtagagt ttatataaca tacaaccaaa gcatttggat
    10681 actgttgctt taattttatc acgccttctt catctaccat atctgccata gcacacccag
    10741 cgttacttac tggcatcaat acagtttttt caggtgataa tatcttagca ctttctccca
    10801 taaaccttac tccacaaaat acaaccactt cttctttaca atctctagca atctcactta
    10861 aataatatga gtcaccaact gcatctgcta attcttgtat ttctggtggt tgataaaaat
    10921 gggcaagtat tattgcattt ttttctttct ttagctcttt gatttgatat gttaaatctt
    10981 tatccataat tgcaccgctt tctatatgta ctagtgtata tacacctgta aacccattat
    11041 atcacttgac tattctttct tcaagaattt taaacctctt aatattgtta agatatagac
    11101 atttatattc tcaattgatt ttcctttttt ctccatctat ttaaactaat ttttatattt
    11161 tttaattttt ttgtaaaata ttatcagaat tttgtttata ttctttattg ctataacatt
    11221 gaaaatatta ccaaatacct gtatactaag tacatggtta tgtatatgta tttatttctg
    11281 aaattatatt agttgtaata ctttatagaa tttgtaggtt gtccagaatt aaattcaata
    11341 taaatctaca catttagata atatgtatac aaagaccaaa aaattttata aatggggtga
    11401 tattatgaca acattcttaa tcggcttggc tatattacta ataggaggtg ctttatatgg
    11461 tgcttattgt gaaaaagtat ttggcccaga tgacagaaaa acacctgcac ttgctcaaag
    11521 tgatggagta gactatgtcc caatgaaaaa atggaaaaat agtctaattg agttgcttaa
    11581 tatagctggt acaggaccaa ttctaggacc tatccaaggt atattgtttg gtccaatagc
    11641 attcatacta atacctatcg gctgtgtatt tggaggagct ttgcatgact atatgagtgg
    11701 aatgattagt ataagagaaa aaggagcaca aatgccttct cttatcagta gatttttagg
    11761 taataaagta tttcaagtct ataatatatt cctttgtctt ttaatgcttt tagttggtgc
    11821 agtttttatt tatacacctg gggatttagt tgttactcaa attttaaata tgaaatcgac
    11881 tataaataat ccagttgtct ggatagttta tggacttatc tttctatatt acttgtgtgc
    11941 aacactattt ccaattgata aaatcatagg aaaggtatat ccaatatttg gagctattct
    12001 acttttatct gctgctggtg ttggaattgg aatctttaca caaggttatg atttagctaa
    12061 tttaagtctt gcaaattgga aaggtattca tcctgatggt attcctctta ttcctacatt
    12121 ctttgttaca gttgcttgtg gtattgtttc tggattccac tcaactcaag ctactcttat
    12181 agcaagatca gtttcaaatg aaaaagaagg aaaaactact ttttataata tgatgatttt
    12241 agaaggttta attgcaatga tttgggctgc agctgctatg ggtatttaca ataaaggaat
    12301 acctaaagaa ttagtaggtt ctcctgatgt aataggtttg gttgcaagag atttacttgg
    12361 ttctattggt ggtatcatag ctataatagg tgtaattgtt ttaccaataa cttctggaga
    12421 tacagcttta agatctttaa gattaatgct tgcagattac tttcattatg accaaaagga
    12481 aaagaaacat cgtgttatat tgtcaatctg tatatttatt cctgtcatag ccatccttat
    12541 atttgcaaag cttagtgctt ctggatttaa tatattgtgg agatacttct cttggagtaa
    12601 tcaaactatt gccatatttg catttgcaat gattacagtt taccttataa taaaagaaaa
    12661 gaattatatt ataagcttaa ttcctggtat gttttattcc tttgttatat tcagttatat
    12721 atttaatgct caaattggat ttaacttaaa cataaatata tcatatgttt tagctgccat
    12781 atttactgta ttatatgcta ttttaactgt acgctctggt agaaagttaa aaagcaaagc
    12841 agatactaag ttggctgact aaatctaaat aaaataatac ttagaataaa ttttattaaa
    12901 aaatgataaa aaagtaggca atctcaagtt agatttgcct cttttttatt taatacagta
    12961 ataaatactt aagtatttac tccttattgg aatatttatc ctaaatctct aatataaata
    13021 aataaaagtt ttaaaataat ctattcattt atatacttaa aataaatttt ttggaggaat
    13081 acttatgaat aataaattac atgaacttga aaaaaatcta ccagaaatat cttgttcttt
    13141 ttgcacacat ctttcattta aggggcctag cctagactat agatatgata ttagatgtgt
    13201 tatttcagat acaaagcccg attttagagg atgttgtgaa tattttgaac cagaatatac
    13261 agaattaaat acaggagatt tagacaatct atacataaac tttcttgaga catgcctcaa
    13321 ggtaaattat aatgattaca tcaattctat gtattggaag ttatttaaag aaagaaccct
    13381 actagaaaac aattttaaat gttctatatg tggatcaact gaaaatgttg atgtgtatca
    13441 tgttaataaa aatttaggaa gggaaacttc caaagatgta gttgttaaat gtgataaatg
    13501 taattgtaaa taagtgtata aaatttagat aacttcacat aataagttta aataaaactt
    13561 aggaggttat ttatgaaaaa taataattta aattgtgctg ctactaattg tgcttataat
    13621 actagtggat attgttatgc tggtagtata aaagtagatg gaatgcaagc aactactaca
    13681 gaaaatactt attgtgcttc ttttgaagac aaatatacat ctggtataac tagtcgttct
    13741 aatgaaacta accaagttga tacagataac attcattgtg aagctgtaaa atgtaaatac
    13801 aataaaaatg aactttgcaa agctgaaaaa gttcatataa atgaaagaaa tgcaagttgt
    13861 gaaacttttg aaatgaaata aatttctttt tatttagttt tatagtattt atacaaagca
    13921 aaattgaacc tatcaaatag atgtctttat taatgatgtc tatttatagg ttctttttta
    13981 aaacaattca taatttatat ataaaaataa attattattt taatatatta cctagaagta
    14041 aatttaacac ccagaatatc cataaggaac ataaatattg gaactccaat tagtaatccc
    14101 catacaccta aataatgctc tcctacaagt aaaataataa aaacaaaaca aactggaagt
    14161 ttagttctat tcgacatcaa cttagggttt aatatgtatg tttctactgc atgcactata
    14221 caaatcataa gtaaaactcc aaaaaccttt gttattcctc ctgtattaaa tgctattaca
    14281 gttaatggaa tcaaagacac tataactcct gctactggaa taagacccaa cacaaaaatc
    14341 ataaatccta aagcccatat ttgagggaat cccatgaaac ctaagaaaat catagaaaca
    14401 acagaattta taaaggcaat cataacttgt actttcatta cttcaccaaa atttttaaca
    14461 aagctctttc caaaaaatac aagcgaatta taaataaaag aaatcttact atcagaaagt
    14521 ttatctccaa aatttttaat ttcatttttt tcaagtaaaa ggagtaaact cagtaaaaat
    14581 gccaataata tatttactcc aaaacttcca acttttgttg ctgttgatgc tataagtagc
    14641 cctgcttgtg aaatatattt attaaaatcc agctttgaga caataggata caatttcggt
    14701 cctaaagatt gagcaaattg atttacatca aaatcactaa aaaccctact caactctgta
    14761 agttgaacta taattattgg tacaactgca tagctaatca atactaacaa tatagcaaat
    14821 agtgcatata acactataat tatcaatgaa tctggaatat ttaaagaaga aaacttctta
    14881 attcgttttc ttgtggctac aagcaagtta tagaaaataa aagtcataat aaatgttaaa
    14941 agcaccatat ttaacatata ccaaaatgta attagaataa ttactacgac agatatagaa
    15001 acaattttat attttgaact gtcacgtgcc aatcctttgt tatcttccat agtttaaatc
    15061 ctccccaaat ttttatgact ttataatgac tgcccttttt attttaatct caaaaaacaa
    15121 gtagaaaaat actaaaaata acttattaat tttcataaaa caatattaaa atttagagaa
    15181 gatacatttt aaataatctt acttatactg aaatctgctc tacatcatat atttaattta
    15241 ttattatcta tctcatatag caattcgtat atctctggat attcaaaaaa caaaccctct
    15301 tttagtaatt gtcttatttc atctgtgctt acccacttta tatcacttac ttcttcctcc
    15361 tgtaaaatag ctttagatat atcatattct tttttaacta agtatacatc ccaaagagta
    15421 tcttcatgaa tcatacttct aaaaactttc atctcatctt tagttatatc tatccctatt
    15481 tcttcctttg cttcgcgaat tgctccttct aaactgtctt ccccagaaac aatacatcca
    15541 gttgtcattc cccacatatt tggcaatgtt tttttacttt tagacctttt ctgaattaaa
    15601 atttgagagt tactatttaa aatccacact tcaactgcca aatggtaata accttcttca
    15661 atagaattac ctctttttat aacatttctt gttttaattc catcagcatt atataaatcc
    15721 cataattcca tttcaaaatc tccttgactt tactataaat tagataaact acaaatatat
    15781 tttagcataa ttaattctgc tcatttttta ttacaatact tgatttacat ttatgttcat
    15841 tgcttttctt ttccttccat tctcttatat catctataat aaatctatgt ttcagcaatt
    15901 ttgtgtcaaa tttaaatatt ctaaacacta tttgtaatac atatcctaaa cctaaagcac
    15961 ttacaagcgt accaatacct acagaacctc ctaataacca tccaacaact aatgctagta
    16021 tttcaagaat tgttctaaca agacgtacag atttattagt ttttttctga agagcaatca
    16081 taagcccatc tctcggtcca cttcctaaac aaacacctaa gtatagaaaa cttgctatag
    16141 ctgccaaaat aattcctata acaaccataa acacacctat ataagtgttg cttgcatgag
    16201 gtatcactcc agaaaatatt actaaatcaa taaaaattcc tataaatgtc atgttgagta
    16261 aagtacccca tcctatattt tcaccaaaaa ctgcatccaa tataacaatt acaactccta
    16321 ccattataga agcctgacct atagttatgc ctagctttat agataaacct tggtgaagta
    16381 catcccaagg cataagacct acatgtgcat taatcataag cacagaagaa attgcaaata
    16441 taaaaaaacc tgaaaataat cttatcatac tttttaaact tatttttccc atcaaaaatc
    16501 actcctcata ataataataa caataaatac caataaaata tatatataaa tacgaaattt
    16561 tacagaatta tttattaggt taagataaaa ttttaattat aaaaagtggc aatccaaatt
    16621 ttatacattt tcacctatac tctaagcttt cgtgtataaa aaacggatat accacttttt
    16681 tagtatataa acatttttag tttttcttat taattttcac tataaattct agcttcttct
    16741 tctccattta gtactcttaa agttccaaaa gcaagtgatt ccaactcatt ttctcctgcc
    16801 ataatttcta ctggtgctat aaactcaact ttctttttaa ccattgaagt aaacatttca
    16861 gaatatgcaa ttccacctgt tataattata gcatctacct ttccatctac tacagttgct
    16921 aactctccaa taccttttgc taactgataa gccattgctt cataaataat ttttgcttct
    16981 tcatttcctt ctgctatcat tttttcaact tctctagcat ctacagtatt taggtatgaa
    17041 actataccac ctttacctct tatcttttta gtcatttctc tttctgaata ttgacctgaa
    17101 taacaggcag ccactaattt tgtagcaggt actcttcctg acctttctgg agaaaatggt
    17161 ccttcatcat cagatagcat atcaaccatt cttccttttt catgaagata aatacttact
    17221 cctccaccaa tgtgagctac tattaagttt aagctcttat aatcttttcc tttatcctta
    17281 gcatatttta aagccattgc tctagtattt aatgcatgcc ctgcacttga tctatccatt
    17341 ccacaaagtc cagatattct cgctacatct ataagctcat ctactgcaac agaatcataa
    17401 atatatgagt tgattccaag aggttctgat attgcatgtg ccacaacagc acctaaattg
    17461 gaagcgtgtt caagtactgg tctatgtctt agtacatcaa tcatttcttc atttactaaa
    17521 tatgctcctg attttactgg tggtaaaact ccacctcttc ctactattgc actaagagat
    17581 tttaagtcta tactattttc tttaagaata ttcaaaactg cttccttacg catttcaaac
    17641 tggtcttgta tagtattata ttttgcaatc tcttcagctg gatggtctat cgtcttcaca
    17701 agaatttgtt cttctccatc atatactgct atttttgtag aagtagaacc tggatttatg
    17761 gctaatattc tgtaagtcat aattttcttc ctttcacctt aatttctata gtctatttat
    17821 atctattata aactatttgc taaccatttc cataccttca tggaaagctt ttatgtttaa
    17881 ttcaacaaat tttggtttaa cattttcact tataattttt tcccaatcaa tagactctaa
    17941 ttgcatagat ttaactagac atcctaataa aataacattc attatttttg aatttcctag
    18001 ttcttcagct ttatcagctg catttataac ttttgcattt aatctactta actctgcatc
    18061 tatatcactt tcttgatatt cagctcctcc gattaaaaca gtcatagaat ctatcctatg
    18121 attatttact aaagcttttc cttctggttt taaataattg aaccatctta aagcttccat
    18181 tttttcaaaa gatactaata tatctgctcc accaatttct ataactggag aatgaacaca
    18241 atctccatat ctaacttgtg aggaaactga acctcctctt tgactcattc catgtatttc
    18301 actcatcttt acatcatatc cagcttccat caatcccata gttaataatt tactagcaag
    18361 tatagttcct tgacctccta cacctaccaa aagtatactc ttagtcatat tatttttcct
    18421 ccttaactat tgcttgtttt ggacaaactt gtgcacatac tccacatcct acacactgat
    18481 ttctatctat atttgataac ttattctctt tatcaaatga tagagctgga catccagttt
    18541 tcaaacataa tttacatcct atacatttat catggtcaac tttacactta gtcttaaatg
    18601 gattattaaa ttcttctata tcttctttag agaatttttt aagtacacat ggccatcttg
    18661 ttattattac tgatggttca tcttctatag ctaaagccca atctagtgct tcatttactt
    18721 cttttaaatt attagggttt ataactctta catgttctat tccacaagct ttaactaatc
    18781 cttctatatc aacttcttta gttttagcac cttgtaaagt atatcctgaa cctgggtttt
    18841 cttggtgacc tgtcattcca gttattctat tatctaatat aaccgaaata gaattacctc
    18901 tattgtaaac aacatctaaa agtccattta ttcctgtatg gaagaatgtt gagtctccaa
    18961 gtacacctac caatcttttt tcattatcat ccttcatatt aagaactttt tgagctccat
    19021 gtgcagttcc aaaagcagaa cccatacaaa caacataatc tattccatta tatggaggag
    19081 caaatccaag agtataacaa cctatatctc caccaactat taaatttttt ctctttccaa
    19141 gttcatagaa gaatcctcta tgtggacatc ctgcacagaa acttggtggt cttggtgtta
    19201 ctaattcaga tttgtattct atagtatcat ttgtttttcc aaatatagct tttcttaaaa
    19261 catctggtgt catttctcca tatggtggta ttacatcttt tccaatacaa tctattccat
    19321 atgcttttat ttgttcttct ataaatgggt cattttcttc tataacatat attttatcaa
    19381 ctttttctgc aaattctttt attttttcat aaggtaatgg atttgtaaat cctagtttca
    19441 tatatgatgc attttttcca aaaacttctt tggcaaaatt acagcacata ccagaggcta
    19501 taacacctat ttttgtatca tttatttcat agtagtttaa atcagtttca tttgagaatt
    19561 ttttaagaat ttccattctt tcttcaacaa cgccccttct tatttgagca tttgctggaa
    19621 cagttaccat tttcttagca tttttaacat actctttaat ttcaacttct tctctatcat
    19681 agcactccac taatcctttt gaatgacaaa gtcttgttgt aactctatat aaaactggtg
    19741 tatcgtattt ttcactaact tcaaaagctt cttttatcat atctttagct tcttgactag
    19801 ttgatggctc aaataaaggt atctttgcaa attttgcata catcctattg tcttgctcat
    19861 tttgagatga atgcattccc ggctcatctg ctgtaataag taccatacca ccatttaccc
    19921 ctgtatatgc ataagtaaat attggatcag ctgcaacatt caaaccaaca tgtttcatag
    19981 aagccattgt tcttgctcct gcaattgaac cccctatagc agcctcaagt gctacctttt
    20041 cgtttggagc ccactctgca actattgcat ctttatatgt tgctatattt tctaatattt
    20101 ctgtacttgg agtccctgga tatgcagatg catatttaac accagcctca taggctccac
    20161 gtgcaattgc ttcattacct gtcatcaatt gtttcatata aaattccctc catctacaat
    20221 tattaaattc atagtccacc caatttgttc aatgaaatta attatttatt tttacttcta
    20281 aactttcaag tgcttctttt ataatacttg gtgctttctt acatttatct tcagaaactg
    20341 cacaaactgc aaatcttaaa cccattttta atggaactgc aaataaatta tgtttagtca
    20401 attcttcaca tacttctttt ggattatcac atggtatact tacaaagaaa cctgctatat
    20461 atggaagaat ttctaatcct actctttcag cttcttttac aaatacatct gctcttctag
    20521 taagcatgtt tttgtatatt tctttttcat cctcatattc tttgaatttt ttagggtcat
    20581 ttacaatgtt agataatact gccatggcac ttctattaca atttgaccaa tttgctctac
    20641 atgaatgaac acatgagtaa tggaactctt cagctacatc ttcacttgaa gatatacaaa
    20701 ttatagcccc cattctcata ccataagctg taaatccttt tgacatactg aatccaacca
    20761 atacgagaac gttttcaggc aaattagaaa atttcttaaa aaactttcta catacatcat
    20821 catcacctgc aaagtcaata tatgctgaat ctacgaaaaa tattatcttt ttgtctttat
    20881 cttttgcgac ttcttttgat aagtctaata ttttatccca ctcatcatct gcaacactat
    20941 atccagttgg attatgcgct ggtgtgttta taattgtaaa gactctacct tgtttttctg
    21001 ctatatttac aaatttttct ttaaacgact caaaattaaa ccttctattt tcatcaaaaa
    21061 gttggtaatt aactactttt ctattagcct cttcagctat acttacatat ggactccaga
    21121 accaatcaga tgttaatacc tcatctcctt cattagtata gttccataca gctaatttta
    21181 ttgcaccact tcctcctggt gaagctaata ctcttatatg tccttctggt aagtaatctc
    21241 tgaaaaatac ttttttaact gcttctaagt aatctggttg accttcaaga gctgcatatg
    21301 cacctatctc acagttatct aatgctttat attcttcata tacagttttc attgttatca
    21361 attttcctga atcatccatt agagcgccta ttgttgcatt tattacatta tccataccaa
    21421 aagccttctc tgctgcttga gctctgccag atatgctaaa tataacatca ttttcttttg
    21481 gccacattgc atgcttacca accatacttt gactcatacc atcaccccta aaattgtatt
    21541 tactcttaca ttatatatta agcaattact atgccaataa atcccacttt atgtaaaatt
    21601 gattttgttt agaattatta tttttttcat tattaaatta agtgttttaa ctatttttct
    21661 tataaatata tatgcctgtc taaaaaaata aaaatttgca aaaaagtaat gttaattttt
    21721 cttaacactt gttttaaaaa attaacatta cttcaaaata atttatatgt aattatattt
    21781 aaatattttt attgtatttc ttccataaag atgtcctgct tattccaaga acttgtgcaa
    21841 cttcattttt agaaagtcct tgagcaaaaa gcatacttat tattttttct tcaacatact
    21901 tatttacttc ttttatatct atacttatct taccatcatt tatcttactt atatttatag
    21961 attcatcttg gacttctaca cttgctgtcg tttcagaatt aaatttgtct acttcagagt
    22021 taggaagtaa ttttttagtt ttaaaatctc tatcttgtct caatttaatt ttacctgtca
    22081 caacatattt ttgagctatt gtctttaact ctcttacatt tccaggccag ctataattat
    22141 atagcttatc catttcatct ttagtcaaaa caaaattttc atctatactg tttattccat
    22201 tgagaccatc atcctttaat acttcattta caaaattgtt aaacaaaggt attatatctt
    22261 ctcttctgtc tcttaaagga ggtatattta tctctaaact actcaatcta taaaataagt
    22321 ctgctctaaa tgtacccata actatttttt cggttaaaga ttcgttcgtt gctgctataa
    22381 tccttatatc cagaggtatt atataatcag aaccaattct cattatttga cgttcctcta
    22441 ttactcttaa tagttttgtc tgaatattaa aggatagact atttatttca tctaaaaaaa
    22501 gagtccctcc atgagcaagt tcaaatagcc ctctttttcc accctttcta gcaccagtaa
    22561 atgcaccttc ttcatagcca aaaagttcac tttcaagtaa attttctgct atagtagcac
    22621 aatttatagc aacaaaaggc ctatcttttc ttttacttat attgtgtata ctatgagcta
    22681 taatttcttt accagaccca ctttccccat aaagtaaagt tgtataatca ctttttccaa
    22741 tttttttagc ttcttcaata aattctttag ttaatttatc tttaaataaa aaatcatcaa
    22801 aagtatatct agcatagaga cctttttgat tcaagtcaaa tcttattttt ctttctaagt
    22861 tttgtagctt ggttatatct tgtattattc ctaaaactcc ataagtacta ttatcaactt
    22921 ttatcaatgt tgtcctagta ttaacaatta gattatttat atttcttatc tttctatctt
    22981 ctacgtcctc tttttcatgt aaacaatcta acatccactc cattttagga aatacatcta
    23041 agatgtattt attaagtgca cattttctct ctactttaag catgtttttt gcactttcat
    23101 tgtacaaaat tatactgcca ttactatcta tagcaattac tccatctttt ataccatcaa
    23161 gtatatttcg taacacttca ttattaaact tttcttcacc taatgtatct aataattttt
    23221 tacaatattc tacagcttct cttatagatt catcactagc agttgcaaat acactatcaa
    23281 ttccatattg ccttgcaaaa gaacatgcca aacctcctcc tacgattaca atttcatctt
    23341 tttggtctat atattttaca actttacttc ttatctcata cttagattca aaccattctt
    23401 cagttatttc agtacttatg acatttctcc agccaacata gtcaaaagaa acttcattgt
    23461 ctccaagaat caatactact tttttagaat atttctttgc tatttctatt gctcttaaaa
    23521 ggtcagttga cttcatattc attggtataa caggtacatt tactgtatct attactaaac
    23581 tataacctcc acctctacca attatagctt gggcaccttt ttcaacaagt atacgacctt
    23641 gattttccat taagtctagg ttaagtatgt cgattattat agttccattt tctacatctt
    23701 ctctatataa ttcttctatt ctttctttta attcaatatc agaggctata attcctattt
    23761 tttttcgcaa caaaatcacc tcaattttaa attataatgt attgatttct acctgctttt
    23821 tttgatttat ataggttctt atctgcatct tcaattaata tttcaacatc ttttctacta
    23881 cgacatcttt ttatacttac tccagcactt atagttatat agttttttat tttagaatat
    23941 atatgttcaa taccctcaaa ttcaagaagt ttgaaaactc ttttaatata taattctata
    24001 ttatcaatct cttcaacacc gattgattct atagaatcta ctaatattaa aatttcttct
    24061 cccccatatc tgattatgtg ttcggtaata tctataaata cacttcttat aatatcagtt
    24121 accttaacta taactttatc tcctgctaga tgaccataat tatcattata acttttaaaa
    24181 caatctatat cgaccagtat tgcgaccata tttttgtctc tggtttcatt aaataaatcc
    24241 aactgctgtt ccagatatct tctattatat agtttagtaa gtccatcttt taaaatatca
    24301 ttttctaatt tcttattatc atccactagt aatcttaaac tctcttgttt ttgtctgagt
    24361 tcatattgtt tgtgaattgc ttgagatgtt ttataggaat tatttttttc ccacctatca
    24421 gaataccaat ccatactttt ataatattct attgccagtt taaaatcttt ttttagctca
    24481 tatccatttg ctattagccc ataaaatata gatatttttc taagattcaa tgaactcttt
    24541 ttaactaaat ttaaaccttt tatataacaa cttatacctt catctatatt accaaatttt
    24601 atatataaat ctccatacaa cttatatatg taatctagtt tagaattata tataaaaaca
    24661 tctccaaaac ttttatatat attttttgat aaaattaaat aggtgtttac cttttcaaaa
    24721 ctacaattat ctttctttaa ctcaatttca gctgaatata aaaatataaa tgaatataaa
    24781 taatccttat atacaccttc aacaactttc attaatttta tagtctttaa taaaataact
    24841 tctgcttctt tattcatatc ttcttttaaa tatgcccata taaggtctat ttttatagac
    24901 actctttcag ttactgatat tgagttatta ctgtgtgcaa catctaatac attatttatg
    24961 tattttatag cttctacatt atttccactc tctaaataac acaaacttat tatacattta
    25021 caaaaagcct ctatagagta taaattattt tcttgagcca attttagaca ttcaattaag
    25081 tattccatac tttttataaa gttattaaat aaatatagat aaactctccc aaatatataa
    25141 tttgccctac ttacaagagc tttatttttt aaagttttaa aaaaagacac ttcttctact
    25201 aaagctatta catccgtttt ggtctgatat aaagcactta tttctacaca taaactcata
    25261 tttaacaata ccaacaactc atttaaatga ttttgtctta taattttttt tgctgagaaa
    25321 taaaaattat ttaccttatc atattttctt atagaaatag ctgataaagt aagataaata
    25381 taaattttag atattatatc tgaatcatct tttaatgaca attttttaat atactttaat
    25441 gactctatca aatagttgta tgatgcttct ctatcttttt ttatatagtt tataagacta
    25501 attaaatata aagtatagac tctactatta tcttctaatt ttgtatttaa aaggtctttg
    25561 tgataatcca agatgtagtt taaatctgtc aaatttagat agaatttagt caacatatcg
    25621 tattctatct tgtttatatt tctctttaaa tcttctatta aactcctttt caagtaaatc
    25681 accaatccta tttcaacagt taaaaattct taaaatcatg ataatgcaaa catctatttc
    25741 taccactttc ctttgcttta tataaagctt tatcagcatt ttcaattatt tcttttatat
    25801 ctatactact atttttacat gtatcaatgc caatactaac tgtcaaatta ttgtttaaaa
    25861 atttctttac aatcctacat aattttcttc caaatatttt acttttttta taatctttac
    25921 atacagataa tatcagaaat tcttctccac cataccttat cataagcatg tcttttttta
    25981 gatatttttt tattattata gttatatttt taagagcaat atctccatta tagtgtccat
    26041 aagtatcatt gtattcttta aagtaatcta catctatcat aaatgctgtg caatattcac
    26101 taacttcttg tttttctaaa aaatcgttca aaaaagttct attatatgca cttgtcaaat
    26161 ggtcaacata ctttttttta tctaaatcaa gttttacttt tctcatgtta tttatttctt
    26221 tttctatata ctcatgtcta taatttaagg aatctatata atttctattt cgagctttta
    26281 tatacatcat ttttaactca taatacattt taaagtattt catactcatt tcataatttt
    26341 gaatcttttc aaaacacaaa tatatactct tgtatgctaa cttcacataa ttataatttt
    26401 tagcattttt agatttttca aggagttttt tatttgtgtt taaggcatta tcataatatc
    26461 caaagatata atatatcttt gcctggtgat aaattatttc taaatcatat attatatatt
    26521 cttctatatt ttttatcata tttaatatgt ttttagctct atctaaatag catattgatt
    26581 gttcaaaaga tttttcatta ctttttgcat aatacatggc taaacttata aataaatcaa
    26641 acttctctac ttcttttaag ctatgatttt taattttttc tagatatact tcagattttg
    26701 ctaaattatt tattgcgaca ggataatttc tagtctttat taatatttta gttatgttgt
    26761 ttgatgctct atatttttga attatattta catttctata ctttttattg cttattactt
    26821 cattgaaata ttccatggaa cattcataat tttcaaacga acattcacac tcacctatct
    26881 ttatattaca tagtgaagat acttgcatat cctttagtaa atctgcatat tttcttgctt
    26941 ttaaaaaatt taccttagca attagagcat catccaaaaa caaaaaatat attttaccaa
    27001 taataaaaaa atatattttt ttacaattaa aatcgttaat ttcttttatc tttatataca
    27061 acgtttctat ctctttaatt attacagaag attctataca cttacttgcc ttaatgtaaa
    27121 taagttgtga tttaaaaata atttcttcaa ttagaaaatt attttcatca caaataagat
    27181 taaatttatc catatataaa tttgccttat ttacatcttc attttttaaa cttaaaatag
    27241 ctatttgtga ataaaatcgt gctaaaaatt tataatcttt gggtaagcat ttatcaatat
    27301 atttacagga aatcaaaata tactctttca ttttttcact aaacccttga ataccatata
    27361 ataaagctag caaaaaattt gattttaata acattaaact attttgttca gttttatagc
    27421 aatcaattaa attctttact acatcaattt tattttcaat aaaatcgtta ttagtcaata
    27481 atctttcatt caaggttagt ttaaacattg aatctaaaat tgtatcatct atctcttcca
    27541 aatatttact atatattgta actcacctcc gacttacatt atctcataaa tttagtaatt
    27601 ttccatacta tttttgaaaa aagttgatgt ctcaatttag aaatttctct taaattaaga
    27661 catactttac actaaactat acaattttct atctgaacta tataatcctg acaattttcg
    27721 caaattatca cacttacaat ataactatta tcaactttat ttgcccttga tagtttatta
    27781 aaagatagtc ctttataatc aacagatata ttgcttatta catattctcc atctttactg
    27841 aaaatataaa agtgatattc taaaaaagga aagaattttt tcttttcttt tactccttct
    27901 aaacataatg gacatgcttc ttgataatat tctacatcaa aatcaacttc ttctgcttca
    27961 ttaagtatat caattatttt tgaaatactt actccttgaa ctggattttc ttcatcattt
    28021 attaaagcac tacagttctc ctcttttaat aaatattcct tatcatcata tataaactta
    28081 tataaactca tattatatcc tccttataat tattaatctc caagttattc taccatttaa
    28141 ataaagttaa taaaagtaat tgtttataat ctttataatt aagttatgta tacatatatt
    28201 cctatgcgct atcttaatta atgattttaa aatccaagtt tgcactttga acatccagtt
    28261 tcccaaaatc tattgtcaaa actcacttta gttattaata taatgatata aatatataac
    28321 tgcatagatg ttacacactt aactatttaa tatgatacga tttggtatct aaatttatat
    28381 attgtatata gggggagata tatatgtata aagataaaag tgatgaatgt atacacttaa
    28441 tgactgcata catcgatagt ataagtggat attattcatt tattgacaca caattagagg
    28501 attttatgat gaaatatggt gaaaacattg ttgattcaaa tttacacagt ataatgatgt
    28561 tgctttgtaa atggggatta tcttagcatt tcaagaaact tttttgtata ttgtattcta
    28621 tattatttat tcattaacaa aaaagattga cataaactta aaagctatat atagatttct
    28681 ttaaaaatct atatatagct tttttaattt tttcaaaatg tatacttaaa taagttagtt
    28741 tttttcaaaa tttgactatc taatatgata taatttaact atctacaaca ataaaaaata
    28801 tctaatatgt attatctagt ttagctcatc ataataacat ccctatattt acaattaaga
    28861 tattttcaat atagtaatac tcaattttaa tattataaaa aggagagatt ttgtatggat
    28921 tacaatgact tactaaaatc agctagagag aactttaatg gaacatgtaa agtatgtaaa
    28981 atttgtaatg gattagcttg tgctggagat gttcctggta tgggtggtaa aggaagtggt
    29041 tcgtctttca ttgaaaatag aaaaagtcta gaaaaaataa agataaatat gagagtcata
    29101 cataatgttt caaagccaga tacatccata gaattatttg gtagaaaaat gagttctcct
    29161 atattcgctg cacctgtatc aggtactata ttgaacatgg gtggtaaagt ttccgaaaaa
    29221 gaatacatag agcctgttgt tagaggttgt tctaattctg gaatttatgc tatggttggc
    29281 gatactaatg tagatacttt tttactggat aatttagatg tattgaagga taatagggga
    29341 aatggaattg tatttataaa gccttggaat aattcaaaga taatagataa aattagattg
    29401 tctgaagaag caggtgcttt tgcagttggt gtagaccttg atgcttgtgg acttattaat
    29461 aatcaatttc aagaaaatcc tttttctcct aaaacaattg atgagattag agaattggtt
    29521 gaatccacta gattaccttt tattataaaa ggaattatga ctgtagatga tgctttgatg
    29581 gctgtagagt ctggtgctag tgccattatt gtttcaaatc atggtggaag agtacttgat
    29641 tatactcctg gtacttgtga ggtacttcca gatatagcta agtctgtaaa gggaaagata
    29701 acaatacttg ttgatggtgg agttaggact ggtgttgatg tagttaagat gcttggattg
    29761 ggtgctgatg ctgttttgat gggaagacct tttgttactg cttcttttgg cggtggtctt
    29821 gatggtgtgg agttttttat agagaaggtt aggaatgagt tatgtgagac tatgattttg
    29881 actggttgtc aaaatgttaa ggatatagat ggtagagtta tatggaattt attgtaaaat
    29941 agttttataa catttatatt taaatagtgt ctcatttaaa ttgaaaaatc atattgagac
    30001 actatttaat atatttttag attttaatcc tatgaaagag tagggtcagt tagtctaccc
    30061 aatttatccc aagtagctgg accaattatt ccatctgcag acaaaccgta ttttttttgg
    30121 aaacttacaa ctgcattata tgtcttactt ccaaaagaac tatctatacc tacattataa
    30181 ccaagtaaag ttaacatgat ttgtgcagct tttacttctt ttccgctatt accctttcgt
    30241 ataacttttg ttgtacttat agtcaaatac tcattactca taattgttat agttttagca
    30301 cctatattat aagaaacagt ataacttgca taaacacctt tactattgtc ctcattcatt
    30361 agattagctg attctggaac aaatatctca tcatctggct tagggtctat atttatctct
    30421 tcacaatttt gtttatgttc atctcttatt tcttgtggcg cattttcata tgtatatata
    30481 tacccagaaa gagtaaaaga atctaggttt ttctctggaa cagtctcttt acttcctcct
    30541 ttctctttac ttgtgtcagc aaatgccaca cttgtaaaag taagcaataa gcaacacata
    30601 atagctacta tttttcttaa ttttgttaac ataaaatcct ccttataatt attttattaa
    30661 ttgattataa tctttcatat ccatttctat tatgtcatag tctctaggta ttttaccttc
    30721 taattcgcat ttatcttcat aaaatttcct aatttcattt ggtgcatctt tataataaat
    30781 atttataatt ttatcagtcg tcttattcaa taaaaattca ttaatattgt tattcattcc
    30841 attattaccc tcatcaattc taacttgagc atcaacttca acaaaagatg ttatagaagt
    30901 cataaatact aaaacagcaa ttagtattcc acttttgcct ttctttttaa caatactctc
    30961 aagtctcttt tccaaatttg tcttatcaaa ctcggttgtc aaaatcttac tattataatt
    31021 tttactaaac ttaattgact ttataagtgc tagtgcatat tcttttttat cttttaaaga
    31081 acattctacc aaaacacttt catcacaaca taattcacaa tctagattta ctttattgct
    31141 cattacatat acaagtggat taaaccaata tattattcta acagacaaaa ctaaaaactt
    31201 tattaaaata tctttatttt taaaatgcat taattcatgt ttcaaaatcc aagttagttc
    31261 atctttacta taaggatagt ctggtaagaa tatgtaagat ttaaataatc ccatacctgc
    31321 tggacttatc aattcatcag aacctcttaa ttcaattttc ttttttatat ttaattcttt
    31381 taacaagttt ttgtagatac agtttatatc attatcttca atatcataag atacatcaac
    31441 aactagattt ttaaatcttg tatactttat aaatgtataa actgcaatga ctatagttac
    31501 aaataaccat aaataagcta catacagcat aagattatta tctgtagaaa tatcaatttg
    31561 tgtaatatta tttcctaatg cattttcttt gggttctttt acttcataca ctattgtata
    31621 attaaataga aaaagtaaca ttctaaagac tattatcaac caaatataat aattgaatct
    31681 cttactaaac ttcttaaata aatatctttt caaaaataat aatagacaaa taccaagact
    31741 acatacaata gttgtcttta taagattttc aaaaacatat agcatataat tattcatcct
    31801 ttaaattatc aaaataactt tctagaaaat ctagcttatc tttacctata ttttcatcat
    31861 catgtaatgc tgaaattaag cttcttaaag agtcatcatg tatattactt aggaaatcct
    31921 ttgtctcaaa acttaaataa tctttgtgtt ttactcttat tgtgtaatgt gtatatctgt
    31981 ctattttttg agcatctaag aatcctcttt ttaccagcct agataaaagt gtcagtgtgg
    32041 tggtttgttt ccagccatat ttttgttcca tagcttcaat aacatctttt gatgtcactg
    32101 taacatctac tttccaaatg aatttcatta cttttagttc tgcttgtggt atttttctta
    32161 tcattattta cacctccctc aaaactcctg ctaccttaat tctacaacat gtgaattttt
    32221 tagtcaaaca ctttatgaaa aaaatgtgaa tttttgtact ttttgttaaa tttctaattt
    32281 ttttacaata ttaaaagtta gattttaaaa tatatctatt taaatatttt actttttaca
    32341 tatttaatta caatagtgta gaatgttatt tacacattaa aactatttaa taaaaattcc
    32401 tatattttca cacaaggaat attataatac ttttttttaa aataaactag agtaatatct
    32461 aataaatttt aataaccttc taattatg
//
LOCUS       DAMKMI010000030        32416 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMKMI010000030 DAMKMI010000000
VERSION     DAMKMI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32416
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.031"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            45..512
                     /locus_tag="PC429_001336"
     CDS             45..512
                     /locus_tag="PC429_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846851.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            555..2318
                     /locus_tag="PC429_001337"
     CDS             555..2318
                     /locus_tag="PC429_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF3846852.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            2326..2934
                     /locus_tag="PC429_001338"
     CDS             2326..2934
                     /locus_tag="PC429_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3846853.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            3006..3575
                     /locus_tag="PC429_001339"
     CDS             3006..3575
                     /locus_tag="PC429_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF3846854.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            4093..7632
                     /gene="nifJ"
                     /locus_tag="PC429_001340"
     CDS             4093..7632
                     /gene="nifJ"
                     /locus_tag="PC429_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF3846855.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
     gene            complement(8024..8920)
                     /locus_tag="PC429_001341"
     CDS             complement(8024..8920)
                     /locus_tag="PC429_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF3846856.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPEDNNNSDSTNKPD
                     DNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYF
                     SHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFS
                     KIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(9416..10750)
                     /locus_tag="PC429_001342"
     CDS             complement(9416..10750)
                     /locus_tag="PC429_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF3846857.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            complement(10812..11588)
                     /locus_tag="PC429_001343"
     CDS             complement(10812..11588)
                     /locus_tag="PC429_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF3846858.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            complement(11693..12358)
                     /locus_tag="PC429_001344"
     CDS             complement(11693..12358)
                     /locus_tag="PC429_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF3846859.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            complement(12358..13014)
                     /locus_tag="PC429_001345"
     CDS             complement(12358..13014)
                     /locus_tag="PC429_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF3846860.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIIEGGNL
                     "
     gene            complement(13023..14201)
                     /locus_tag="PC429_001346"
     CDS             complement(13023..14201)
                     /locus_tag="PC429_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF3846861.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTITVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQSKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            14556..15458
                     /locus_tag="PC429_001347"
     CDS             14556..15458
                     /locus_tag="PC429_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3846862.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            complement(15674..16486)
                     /locus_tag="PC429_001348"
     CDS             complement(15674..16486)
                     /locus_tag="PC429_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846863.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLIDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(16489..17448)
                     /locus_tag="PC429_001349"
     CDS             complement(16489..17448)
                     /locus_tag="PC429_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3846864.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            complement(17554..19113)
                     /locus_tag="PC429_001350"
     CDS             complement(17554..19113)
                     /locus_tag="PC429_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3846865.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            19332..20309
                     /locus_tag="PC429_001351"
     CDS             19332..20309
                     /locus_tag="PC429_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846866.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            20310..21281
                     /locus_tag="PC429_001352"
     CDS             20310..21281
                     /locus_tag="PC429_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF3846867.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            21433..23475
                     /locus_tag="PC429_001353"
     CDS             21433..23475
                     /locus_tag="PC429_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF3846868.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNIMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVIKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATILLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            23736..24578
                     /locus_tag="PC429_001354"
     CDS             23736..24578
                     /locus_tag="PC429_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3846869.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            24881..26953
                     /locus_tag="PC429_001355"
     CDS             24881..26953
                     /locus_tag="PC429_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3846870.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVSTAKEENRVSIL
                     RDDIEKIG"
     gene            complement(27166..28035)
                     /locus_tag="PC429_001356"
     CDS             complement(27166..28035)
                     /locus_tag="PC429_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3846871.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            28199..29653
                     /locus_tag="PC429_001357"
     CDS             28199..29653
                     /locus_tag="PC429_001357"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF3846872.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVAIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEAYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            29925..32204
                     /locus_tag="PC429_001358"
     CDS             29925..32204
                     /locus_tag="PC429_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3846873.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
BASE COUNT        12022 a         4091 c         5219 g        11084 t
ORIGIN      
        1 tataattgta aaacttacaa atatatgtaa aatatttagg agtgatgaaa atgttaaatg
       61 ggataataaa gcttttttat tctgaaattg taaaaacaaa gattaataac ttagattcaa
      121 gtggatattc tttaagagat gaatgtaaag aaatcagaaa tataactcta aagatagata
      181 gtgtattttt taagctttta aagttagctt ttggttcaat attgcttatg gtagctttta
      241 atttaaacat ggttttaggt ataggagtgt ttttagttgg tgtagtttat atactatata
      301 aaagaaatat ggaagaacaa gtaaaagagc atataaataa tgtgaaaaat aatatcgaca
      361 tctctaaaat aacttttatg gatgaaaaag ggagaacagg tctaaatgca ttaattacat
      421 tacttcttat aggattattg acaggattta actggatatt agttgctagt tttgtcgttg
      481 tttttgtttt tactataaag aatgtatgtt aaaatactaa taaatagttt aatatatagg
      541 gggagaaatt gagtatgact aatggcagtt ataaaatgaa gaatttagtg gaaatatccg
      601 ctatggtgac tgaatctaca gattttttta atataaaaga tgacataata gaaaaaatgt
      661 tagaagttgt tcaccctaca aaggcatgtg tgaatctatt ttataaaaat gattctaaat
      721 atgcctactt agtatgttcg caaaccttag aatatgtacc tcaaattttt ccaataaata
      781 gtcttaaagg tgcaaaaatt gattttgaca catatccaga gtatatacat gaggctgtaa
      841 atgaaaaaaa aataatatat gtggaaaatg tatttgaaga ttctagagca gaaggtgaac
      901 gagatttagc aaaagctgaa ggttatattg ggagaatagt ttttcctttt ataataaata
      961 acgtagttgt tggatttatg acttgttttt taaaagagga tgattatata acagaacaag
     1021 atatagattt tatatcatct gttgcatcac tcttaagttt atcaatagaa ataactaata
     1081 aaaataataa tgtacaaatt ttaattgata aactaagagg ctcaataagt gccataaatg
     1141 aagctactaa aaaactatac cttaataaga gtattgatgg atttttggaa aatttaagta
     1201 aacaagcgtg taatattact aatagtaaag aagctttaat aattataaat agtcatcata
     1261 ataaggatgc tatagttagt tgttataatg ttgagcaaaa aaagaaaaca aatttatatc
     1321 ctatgattga tatgttttta aagaaagagt cattgggggg gtatttaaat aatttaaaac
     1381 catgctcaaa aaagggaata aatttggaaa gttatatata ttacaaactt caggataaaa
     1441 atgagactat aggatgtata gtttgtgcaa atagcaaaaa ctatactagt gatgatttaa
     1501 atattcttag tatattgtcc aaacaagttt ctgttggtat gcaattatat gaacataatg
     1561 aaaaggaagt aaaacacaaa gttcttgaaa acgagttgaa catattaaat aaacaacaac
     1621 agcttattat ggataaaggt aaaatggatt gtaatgatga gaaagaactt tatttttacc
     1681 ataaaccagc aagggtagta ggtggagatt tttattatgc tacaaaagtg gatgatgaac
     1741 atatagttta tattgttgct gatgtaatgg gacatggaat ggtatcaaat tatgttgttg
     1801 caatgataaa aggtgctttt aaagtattat gcaatcaata caatacacca agagaaataa
     1861 tgacaaattt aaataggatg ttatatgatg aatttgacaa gatgggagta tttacaactt
     1921 gtttgatagg tattatagat actaaagaaa atatgattac tgtgtctaat gcaggtcact
     1981 atagtcctgt agttgtaaag aaagatggca caatagaaaa taatttaaat tgtaaaaagg
     2041 gcatacctat tggaataatg gaagatgcta cttatgaaaa taatactctt tcaatggaag
     2101 attatgcaat ggtatgtatg tacactgatg gaattttaga gataaaaaat tcatcaaaag
     2161 aagagtatgg tataaacaga ttagaaaact ttctaaaaga aaattttaga ttaaatcaac
     2221 agttaatagt agaaaatcta aaattggatt taaagaactt ctcatcaaaa gataattatg
     2281 atgatgatat tttaatagtt atgttaaagg ataggtagat tgattatgga ttcaaacgat
     2341 aaaaaaggac ttaaaagtcc agagattata ataataaatg gaagtccaag aattaataaa
     2401 aattgcagtt ttatagcaaa tgaagtaaaa gctatgctag aagagtgtgg attaagttgt
     2461 aaaatattta atatctacaa tatggatatt gaatattgca cagcatgtgg attttgtgaa
     2521 aaaacagggt attgcaagtt taatgatgat atgaagccat tatataaaat gtttgatgat
     2581 tcaaaaggaa ctatagtaat aagtcctgta cattttgatt caatatcagc taaattaaag
     2641 actgttgttg atagaactca agctatatat gcaagtaaat atgtattaaa taaaccttca
     2701 attgatagaa gtaagaaacg gataggaatg tatattgcag tgggagggag taaatcatat
     2761 gattcacagt ttaaaggtgg acagattgtt atggattttt tctttaagag tataaatact
     2821 aaacttttac acaatatata tttaaacaat acagatgatg tatcttatat agataatcaa
     2881 gaatttaaag atataatata taaaaattta aagatatata ttgattcaat ataatttgat
     2941 tttgtaggta atattatgta taattgtatt gcaataattt aaataattaa aaggagggaa
     3001 tttcaatgaa attaccaata gcattatcta ataaacatgt tcatttgagt caagctgata
     3061 tagacgtatt atttggagca ggtcatgagt taactaaatt taaaccatta tctcaaccag
     3121 ggcaatatgc ctgtgaagaa aaagtggatt tagtaggacc aaaaggaact ttaaaaggag
     3181 ttagagtact aggaccagct agagctcata ctcaagttga aatatcttta gcagatggtt
     3241 tcccattagg agtaaaagca ccaataaaag aatctggtga tatagctgga actccaggtg
     3301 ttaaattagt aggacctgct ggagaagttg aaatgaaaga aggagttata gttgcttcta
     3361 gacatataca tatgagcaat gaagatgctg ctaaatttgg tgtaactgat aagcaaatag
     3421 taaaagttaa aacatctgga ccaagagcat tagtatttga aaatgtatta gtaagagcta
     3481 gtgacagctt taatctagaa atgcatgttg atatggaaga gggaaatgca gcaggcgtta
     3541 aaaatggaga tttagtagaa ttaatagttg attaatatta taaatagact ttatgctagt
     3601 agatatttta tttgctagca tatttttaaa tgtaataaat ttctatagga taaatttaat
     3661 caattagatt aatttataaa ataaaggggt attataaaaa atgtaataaa aacttacaaa
     3721 aatatgatgt aatatggtaa aattatatat ataaaaattt acatgtaata aggataagca
     3781 ttttactttt tatactaata tgaacaaata gataaaaaag tttgaaaaat ctaacttaat
     3841 ttattatata ttgtatacaa aattatgaaa aaaacttaac atatttaatt ttaaaaaaaa
     3901 tctacttaaa tatgtaatta atttacaatt ttcaaaggaa ttttatctaa tctgtagtat
     3961 tatattagaa gatagagtat ttgcgcgact atatgtgtgt ttatagtttt gttgtgtaaa
     4021 ttatatgtaa atataaaaaa atagacttgt tataaacaag tacaattgta gctatcaagg
     4081 aggatacata ttatggctaa gtttatgaaa acacttgatg gaaatacagc tgcagctcat
     4141 gttgcctatg cgtttacaga tgtagcagct atatatccaa tcacaccatc ttcaactatg
     4201 gctgaagtag ttgatgagtg ggcatcacaa ggaagaaaaa atatatttgg acaaacagtt
     4261 aatgttgttg aaatgcagtc agaagcaggt gcatcaggaa cattccacgg ttctttacaa
     4321 ggaggagctt taacttctac ttttacagca tctcaaggtt tattattaat gatacctaac
     4381 atgtataaag ttgcaggaga gttgttacca ggagtattcc atgtaagtgc tcgtgcttta
     4441 gcatctcaag ctttatcaat atttggggat caccaagacg ttatggctgc tagacaaact
     4501 ggatgtgtat tattagcttc tggttcagtt caagaagtag ctgatatagc acctgttgca
     4561 catttagctg caatagaagg tagattacca tttatacatt tctttgatgg attcagaact
     4621 tcacatgaaa tccaaaaagt tgaattacta gaaaatgaag attatgcaag tttattaaac
     4681 tttgaagcag ttcaagcatt tagagataat gctttatctc caaatcatcc agttgctcgt
     4741 ggtactgctc aaaacccaga tatatatttc caaactagag aagcttctaa taaatactat
     4801 caaaatatag taggtatagt tgaaaaatac atggaaaaaa tgagtaattt aacaggtaga
     4861 aaacatagtt tatttgatta ctatggagca gaagatgcta aatacgtatt aatagctatg
     4921 ggttcagtaa cagaaacaat agaagaaact atagattact taaatgctaa gggagaaaaa
     4981 tatggtttag ttaaagttca tttatataga ccattctcaa tgaaacattt cttagatgct
     5041 atgccatcta ctgttgaaag aatatgtgta cttgatagaa ctaaagagcc aggttcaact
     5101 ggtgaaccat tatacttaga tgtacgtgat gtattctatg gaaaagaaaa tgctcctatg
     5161 ataataggtg gaagatacgg attaggttca aaagatacta ctccttcaga tataaaaact
     5221 gtttttgata acttagtttc agaacaacct aaaaatggat ttacagtagg tatagttgat
     5281 gatgtaacta atacttcttt aactccatct gagccaataa aaatagcttc taaaggaact
     5341 ataagatgta agttctgggg attaggttct gacggtactg ttggagctaa taaacaagct
     5401 atcaaaataa tcggtgacca tactgaaaaa tatgctcaag cttactttga ttatgactct
     5461 aaaaaatctg gtggtataac tatgtctcac ttaagatttg gtgatactcc aataagagct
     5521 acttatttaa tagatgaagc tgattatatt gcctgtcata aacaatcata tgtttatcaa
     5581 tatgatttat taaaaggtct taaaaaaggt ggtacatttg tacttaacac tatatgggac
     5641 caagcaggac ttgaagaaca tttaccagct catatgaaac aatatatagc taaaaatgat
     5701 ataaaattct atacagttaa cgctgttaaa ctaggacaag aaataggtct tggaaacaga
     5761 ataaacatga taatgcaatc tgcattcttt aaattagctg aaataatacc agaagaagat
     5821 gctgttaaat acttaaaaga ttctatagtt aaagcttatg gtaaaaaagg tgaaaaaata
     5881 gttaacatga actatgctgc tgttgatgca ggaataaatg ctttagttaa agttgaagtt
     5941 ccagcttctt gggaaaatgc tgttgatgca gataaaggag aagttaaaga accagaattt
     6001 ataaaaaata tactaagacc aatgactgca caagaaggaa acaacttacc agtaagtaca
     6061 ttcaatggta tagaagatgg aacattccct tgtggtactg cagcttatga aaaacgtggt
     6121 atagctgttg atgttcctga atggataata gataactgta ttcaatgtaa ccaatgttct
     6181 tttatatgtc ctcattcatg tataagacca gtattagtta ctgaagaaga attagctaat
     6241 gctccagaag gatttgaagc taaaaaagca ttaggtaaag gattagaagg attaaaatat
     6301 agaatgcaac ttagtccact tgactgtaca ggatgtggaa actgtgctga catatgtcca
     6361 gctaaagaaa aagctttagt tatgaagcca atagatactc aacttgatac agagttagat
     6421 aattgggcat ttgcagtaaa tcctgatgag gtagctccaa aaggtgatgt tatgcctgca
     6481 aacactgtta aaggaagtca atttagacaa ccattaatgg agttttcagg agcatgtgct
     6541 ggatgtggag aaactgcata cattaaagta gttacacaat tatatggaga tagaatgatg
     6601 atagctaatg ctactggatg ttcttctata tggggagctt ctgcaccatc aattccttat
     6661 acttgtaacc atgaaggtaa aggtccatct tgggcaaact ctttattcga ggataatgct
     6721 gaatatggat ttggtatgta cacagctgtt aagcaaataa gaaataaaat agtagatgct
     6781 atgacagagt tagtttctat ggatatctgt gaagatgcta aagcggtatt tacagagtgg
     6841 ttagattcta gaaatgatgg agaagcttct aaagtagcaa gtgctaaggt tgttgaatta
     6901 ttagaaaaac cagcttgtga ttgtacagat gaaaaagcta aagagttagt aaaagctata
     6961 aaagatagaa aagattatct agttaagaga tctcaatgga tactaggtgg agacggttgg
     7021 gcatatgata tcggttacgg tggattagac catgttcttg catctggtga agatgtaaac
     7081 gtacttgtat ttgatacaga agtatattca aatacaggag gtcaagcttc taaagctact
     7141 ccagctgctg ctatggctaa gtttgctgct tcaggtaaaa aatctagaaa gaaagattta
     7201 ggaatgatgg ctatgacata tggaaacgta tatgtagctc aagttgctat gggtgctgat
     7261 aaaaaccaat ttatcaaagc cgttatagaa gctgaaaaac atgatgggcc atctttaata
     7321 atagcttatg ctccatgtat aaaccatgga ttaaaagaag gtatgggaag aactgtagct
     7381 aacgaagcac aagctgttgc ttgcggatac tggcatttat acagattcaa tccagaagta
     7441 aaagctgaag gtaaaaaccc attcacttta gattcaaaag aaccaactgc aagcttcaaa
     7501 gatttcatac ttgcacaagt aagatattct gctatagcta aacaattccc agaggaagct
     7561 gatggattat ttgctaaagc tgaagctgat gctaaagaaa gatatgaagg atacaaaaaa
     7621 ttagctgaat aatattaaag tattataatg cttatataga attgataaaa gagagtgtct
     7681 caaagtgagc tttttagttc atgaggcact ctttttgtat gaaatcaaat atattttaat
     7741 catttcaaca atgaaaaagg ccttatctct attttgagac agcctttttt tatgaagata
     7801 ttaagatcct gtttttacct attaaatatc tttttaagtt ttccattttc atcttgccaa
     7861 tactttttat atattgctct acacactaaa attaagagaa caaataatat ttatagtatg
     7921 tatatttgga tttgataatt ttaaaataga tacaaaagag tatctcattt gtgtttttta
     7981 tcacttagag ataatctttt gatatgtatg gaaaaagatt attttatcct acaaagattt
     8041 gtgtccaata aattgaacca ccactttttt gtgctactcc aacacctatt ttactgaaat
     8101 ttggattcat tatatttttt ctgtgacctt cagaattcat ccatgcattt acaacttctt
     8161 taggagtttt ttgacccata gctatatttt cacctgctgt cttatagctt attccaaatt
     8221 ttttcaacat gtcaaatgga gaaccataag ttggagaatt gtgtgaaaaa tagttgttat
     8281 caatcatatc ttgagatttt tttgtagcaa catttgatat agaagaatca agagtaagag
     8341 gatttaatcc agctttagct ctttctatat ttactaagtc aacaacttct ttttggtaag
     8401 ctgaaaaact ttcacttgta gagcctgagt tgttattatc atcaggtttg ttagtagagt
     8461 ctgaattatt gttgtcttca ggtttatcag tagagcctga attattgttg tcttcaggtt
     8521 tattagactc aggtttatta ttatcaggtt tattagtaga gcctgaatta ttgttgtctt
     8581 caggtttatt agactcaggt ttattgttac caggcttatt agtagagtct gaattattgt
     8641 tgtcctcagg tttattagtg ttgttgcaat tcttgtcatt atgattctta tccttacaag
     8701 agttatcttt atcaggctta agttctggtt ttacagaaca atttggtttt gcagaaaact
     8761 tgtatttagt attactatat actaaacttt tgttacttat atttacatta ttttcaagtg
     8821 atgaagcact tgcagtaaca gcacttgaag ctgttgctaa agttaacact cctaaagcta
     8881 atatagtttt taattttgtt ggtatatttc ttctcatcat taaacacctc ttaatctaaa
     8941 aattctaaaa atgttactga tttgtaacct ttagtgtagg gggttacccg tgaatctata
     9001 ctaacataag attaagactt tttttactgt aaagatatgt aaaaaagata tatatggaat
     9061 aaataggtat atttcagtgt gtttgacaat ttgtattaaa agcctgtata gtatggaatt
     9121 acttagttag agaaaataat atttttataa taattccata acgtaaattt atagttatga
     9181 taaatttttt tttatttggt taaatttatt cagtagatgc tttcgtatta gcaacaaatt
     9241 ataattattt aaatctttaa gaaataaaaa gggtctgtct caaaatagag ataaggcctt
     9301 ttttattgtt taaatgatag gcatatgtta tttcatataa aaagagtgtc tcattaagta
     9361 aaaagttcac tttgagacac cacctttcat attatgaatt ttaatttgtt ttatattaat
     9421 acattagtaa tcctaatgta attacaataa tttctgctat tagtgaacca actgcaacta
     9481 caatgaaagt ttctttaaat ccagttttat gtgttaactt agataagctt aagaatgtaa
     9541 gaaaaacacc actttgtgga actatagtta gtatatttga agctattgta gcaacacggt
     9601 gaattaattc tggatttaga ccagtactta aataataatc tgcaaagttt ggcattacta
     9661 ttcctaatgc accagaagag gaccctgtta ttgctgacat tgttgaagtc aaaacagtaa
     9721 gacttataag tggactacct ggtattgaca ttataagttt gctaaataca tcaaaaccag
     9781 gtgcagccat tattactgct ccaaatgcaa ctgcagaaga tgtagcaaat ataggaccta
     9841 tagaaccact agcacctatg ctcaatgtct gtatcttaga tgatagaaat ttatggaata
     9901 aaaaagcaga taaaagaatt gcaactatta aagctatgta aattacattt tttttcataa
     9961 attctgaacc aaaagtactt ccagttatag ctataattac gagtgctaat agaggaaaaa
    10021 tacttgcaaa aaatcctggt aattctctat ctaaagattc ttcttctaat tctgaagtat
    10081 aagttgcata agtttcacct tttctaatac ttttgtttag agcatatttc atatagacaa
    10141 gtccaaacgt agcacaacct atactaccta caatacttgg tatagtagct gcagtaagag
    10201 acgtatttag atattgaata ggtataacat tttgaatggc tggtgttcca ggaattatag
    10261 tcatagtaat tgttgcaata ccaagccaaa gtggtatctg aataaggttc caagaaatat
    10321 caagtttttt aaatagagtt cttgcaagtg gaatgaccgc aaacataact acaaataagc
    10381 ttataccacc atacgttaat ataaaactta ctataaatat agcaactaaa actctataag
    10441 gatgattaga gccaaattta tttaaaataa aatcagctat tgatacagta gctccacttt
    10501 cttccataaa tttagcaagt attgagccta ataagaatat tgcaaaataa ctcattatat
    10561 agtttccaag tgcacccata taattgtttt tgtcagcacc tagcattgat gttacaacat
    10621 ccattttatt taaaataaca actattaaag ttgctattgg agcagctata gttatatgaa
    10681 tttctttaac tgaaaaatat ataatagcta tgataccaat aattatacca attggtgcga
    10741 taatactcat aaatatccct ccttagtaga aggacctgaa gaatactttc ttcaagccct
    10801 aaatataata actattgtgc agtatatcct ccatctagta agcaagcttg tcctgtaata
    10861 cccttagcat tatcacttgc taagaacata gcatagtcgg caatttcttg aacatctaat
    10921 aggcgttttt gtggtacaag tgggtataaa acttcctcta agacattttc caatggtata
    10981 ttacgagttt ttgaaaggtc attgaactga cctctgacaa gtggagtatc tacatatcca
    11041 gggcataaag cattaactgt tataccagaa tcggcagctt caagagcagc aacttttgtt
    11101 agtccaataa gtccatgttt tgctgagtta taagcagcct ttccagcaaa tcctattaca
    11161 ccattgattg aggccatatt tattatacga ccaaagcctt gttttttcat tataggaaat
    11221 gcttttttag tagctataaa aggtgctgtc agcattattt ttatcataaa ttcaaattta
    11281 tcagttggga aatcttctat catagaaaca tgttgaagtc cagcattgtt tattaatata
    11341 tctaatctgc catacttttc aacagttttg tctatagcgt ggttaatttc ttcttcctta
    11401 gttacatcac actttacact catgcaatca taaccttttt tttgtaactc tgttatggta
    11461 ttatttaaag cttcttgatt tatatcagag aacattactt tagcaccatt ttttaagaac
    11521 gattcagcta tttgtttacc tattccactt gcagctcctg ttacgaatac aactttatct
    11581 ttaaccattt tcatttcctc caattttatt ttatttttat attaatattt taagcaatac
    11641 aaatttatat gtaatacgtc taagagattt ttattttcta gttttaatta ttttaaacaa
    11701 cagatatttt tccatttaat gaatctgaca atattaaagg agcctcagta gcagaaataa
    11761 catcatctaa tgtgaaagct ggatttattt ctttaagtaa aattccatct gatgttactt
    11821 ccataactcc catttcagta actatcaaat ctacagcatt tgttgcagta agtggaagag
    11881 tacaattatt taatattttt gcagaacctt ttgaagtatg ttccattgct acaataactt
    11941 ttttagctcc agttactagg tccatagctc cacccatacc tggaaccatc ttacctggaa
    12001 tcatatagtt tgctatatta ccatatttgt caacctgaag tgcgccaaga acagttatat
    12061 caacatgacc tcctctgatt attccaaaag aagtagcact atcaaagaaa catcctccag
    12121 gaagtatagt aactggttga ccacctgcat ttacaatagt ttcatcactt gaatttccat
    12181 cttcggctga acctaaacct aaaaagccat tttcagattg taagataacg tttacattat
    12241 ccggaatata atttgctact ttggttggta gacctattcc aaggtttaca acggctccgt
    12301 cttttaattc tttagatact ctatttgcaa tatattcttg catttctaat ttatccatta
    12361 taaattacct ccttcaatga tgtaattcac aaaaatacct ggtgtattaa cttcatttgg
    12421 gtcaatatca ccaatttcaa cgatttcatc tgcttcaact attgtaattt tggcagcaga
    12481 tgccattaaa ttattaaaat tgttcataga gcctttataa actagattac ctgctttatc
    12541 gacctttgat gcaaatagaa gagcaacatc tgcataaatt ggtttttcaa gaaggtattc
    12601 tttgctatcg acaacaattt tttgctttcc ctcctcaaca agagtaccaa taccagtcgg
    12661 tgtgagtata cctcctagac cataaccagc tgcacgtatt tgttcagcaa gagttccctg
    12721 aggaactaac tcaacatctg tttcattgtt attcatttgt cttccagttt ctttatttaa
    12781 tcctacatga gaagcaatta tctttttaaa ttgcttattt acaaccattt tgccaactcc
    12841 tttgtcaata aagccagtat cattgcaaat tagtgtcaaa tcttttatat ttttactaca
    12901 taaataatca attaaatttt ctggtgaacc attggccatg aatccaccaa ccattattgt
    12961 atctccatct tttacatgag atagagcttc atcaatgcta actattttat tcatatttcc
    13021 tcctatcgtg aaaccaccat tgatattcct tgaccaccac caatacaaag tgttgcaagt
    13081 ccagtttcaa ctttacgttt ttgcatttca taaattaagg taacaagtat tctagctcca
    13141 cttgctccta ttggatgtcc taatgctatt gcaccaccat taacatttaa ctttgatgaa
    13201 tctatttgaa gttcattttt aacagctaat gcttgggcag caaacgcttc atttgcttct
    13261 attaagtcta tatcattgat gctaagtcca gcttttttca gtgcttttct agttgcaggg
    13321 attggaccag tacccatgac ttcaggttca acaccagctg aagcatagga ttttatttta
    13381 gcaagtggtc taatacctag ttcgtctgct ttttgttggc tcattagtat taacatagcc
    13441 gcaccatcat taatacctga ggcatttcca gctgttacag taccatcttt tttgaatgca
    13501 ggtttaagtt tagctaaacc ttcaaatgtc atacctaatt taggatattc atctttatca
    13561 atagtttcta tttttcctcg acgtattaaa acatctactg gaacgatttc ttctttaaag
    13621 cggttgtttt ttatagcatt ctcagcctta ctttgacttt ctaatgccaa tttatcttgc
    13681 atttctctag taaattcaaa tttagttgca atattttctg cagttattcc catatgatac
    13741 tgattgaaag catcaataag gccatctgtt agcatactat ctaccatggt tatgtcaccc
    13801 attttactac caaagcgagc agttggaacg atataaggag cttggctcat attttcagta
    13861 ccacctgcaa ttaccacatc attttcacca gatgtgattg attgggcagc tagctggaca
    13921 cttttaagcc ctgaaccaca taatttattt actgtgtagc ttggaacaga atttggtata
    13981 cctgcattta tagcaatttg tctagcaaca ttctgtccaa gccctgtttg aagaacattt
    14041 ccaattatta cttcatctat ttcagataaa ttaagtccta cacgtgatat cgcttcttta
    14101 acagtgatag ttccaagttg aacagctgaa gtatttttaa atacacctcc aaaacttcct
    14161 ataggtgttc tgactgctga tacgattact acatctttca tatctaatag ttacctttct
    14221 tgaatgataa ttaaaaattt tctgaataaa gaaattagga aacactataa taaagtactt
    14281 ttttaataca agcttgtttt taagtatttc aattactttt attcctaatg ctttaggttt
    14341 tcctgtttct aattatagat tacggcgaaa aaagcgtttt gtctaagtct aattttttat
    14401 atagtgtata agaaaaattt ataggataaa aatttatagt agatttagaa aaatattttg
    14461 ttaaaatatt aatgaaatta tgttgatttt cctataaaat atcattagaa gaaaaaatat
    14521 aatctaaatt atacttttgg ggaagggggt ttaaaatgga tataaaacag ttgaaatatt
    14581 ttgttgaaat agtgaaatct ggttttaatt tatcaatagc atctaagaca cttcacatat
    14641 ctcaaccagc attaagtcaa attataaaaa attttgaaga aagtgaagat gtatatttat
    14701 ttgaaaggta taaaggcagg ttaaatgggt taacaccagc aggagaaaga ttttatgtaa
    14761 atgcagaaca tatcataaat gaatacaaaa atatgatgga agatttaaga gaagattcag
    14821 ttcaatttaa aggaaaagtt agaataggaa tacctccact tatacttgga atagcatttt
    14881 cagatgtagt tgcacagtta gttgcaaata atccagatat agaatttgat atagtagaga
    14941 agggtgcata tgacttaaat agaatgctta tcttacagga attagattat gcagtattgt
    15001 tacatccaca taaaattgat aaaagtgtag taactgaaca tgtgcttcaa gaagatgaac
    15061 ttacagcatt tttaaatata aatcatccac ttgctaagaa ggataagata gattggaacg
    15121 atttgaatga gcagttactt gcaatattta atcctacttt tatgatacat cataagctaa
    15181 tgcaaaaatt tactgatgaa aatgttaagt taaaaagata tataatgtct ggttcatggg
    15241 attttttgtt gctatcaacc acaaattctg attttataac aattctacca tcaccagttc
    15301 atgatttttt tgaaaatagc aaaattatag aaaagccttt taatcatcct attacatgga
    15361 aagttatatt atgtagacct aaaaaagaac gttatagcca tgtagaccaa catgtattta
    15421 aatttattat agatttcttt agtaaaaaga actcttaaaa atgttattaa aataacaaat
    15481 aaataaatca atttttatta taataaacta ttagtttttc atctatatgt ttgagagcaa
    15541 taataggttt tacataacat ggtagagtag tatttaaagt tataatatac aaaatatgta
    15601 tttttttaaa cgaaaaataa aaacagtgta tatctttaga taatcactgt ttttattttc
    15661 tatataaaat ttgttaagta ttactagaaa attttttgct tattatagtc aaactcatga
    15721 ctgttaaaaa tataagtaat cctggaaata cagctaaata taacttgtca aaaatatgtc
    15781 tttgagcatc ttgaagcata ttaccccatg atgcaagagg caattggatt ccaagaccta
    15841 aaaaactaag agcagattca gttaagatag cattagcaat atttaacgaa ccagcaacta
    15901 caatagtctc ttttaagttt aaaactatat gtcttaatag tatatttcta gtcttgatac
    15961 cagtagcttt agcagctaga cagtattcat tttcctttat ttttaatgtt tcgccacgta
    16021 caattcttga aacttccatc caacttaaga atccaataat tagtataata gtggaaactt
    16081 taggtgttag ataagtattt aatacaatca aaactaagaa acttggtata ctcataagca
    16141 tatctattaa tctcatcaag aattggtcta gcttacctcc aatatatcca ctaataacac
    16201 ctacagcagt accaaatact acagaaataa gcattgataa aataccaaca ctaagtgaaa
    16261 tccttccacc ataaagagct ctagagaagt aatctctacc aagttcgtct gtaccaaaga
    16321 tatgctttaa gctaggttct tgaagtttag aagtagtatc aattgcatta gagtctatat
    16381 ttaatataaa agaagctata attaatatga ttattatgct aagagtaata catgcaaaag
    16441 caaaggattt atcttttttt aattttttta agatacttgt gaacacaact agaatcctcc
    16501 tttgctagat tttattttag ggtcaattaa taagtatagt atatcagcaa ctaaattacc
    16561 taaaattaaa aatagtgctg ataacatagt tattgccata attacagtat aatctcttga
    16621 aaaaattgaa tcaatcatta atcttcccat acctggccaa gagaaaacta cttctgtaac
    16681 aaatgcacca gtgactaatt tttgtatact catgcctagg attgtaacca atgatgtcag
    16741 tgaatttttc aagacatgtt taaatagtat gactttttct gataatcctt ttgctcttgc
    16801 tgtaataaca tattgttttt ttaattgttc atttacacta gcttctacat aatttgtaaa
    16861 aatagcagta ttatatagac ctaaagttat tacaggcaaa actaaatgtt ttactaagtc
    16921 tccaatacta tcatttccaa tagtatgcat acctcctgat gggaaaatgt taagtttaac
    16981 tgaaaaaatt gaaatgagta tcatacctat ccaaaaagaa ggtatagaaa cacctatata
    17041 attaaaaaca gatgatactt tactagttat tgaattttta tttacagcta aaaatattcc
    17101 aagaggtata gctaccaata ttgaaaatat caaagaagta cccataagaa taaatgtatt
    17161 tcccattttt tccataattt gttctgtaac gggcatatgg tttatataag aaatacctaa
    17221 gtttccactt aaagtgtttt tcaaccagtt taaatatctt atatatactg gtttatcaag
    17281 ccccatatta acccttatat tttcaatatc ctgtgaagac attaaaggat ttatatatgc
    17341 atctgctggg tcaccaggag ctaattctaa aagtccaaat gataatatag atattaagat
    17401 taacataggt attaaaactt ttactctatt caatattaat tttaacataa gtaaattctc
    17461 ctaattaaaa gagatgtctc gttttaaaat aataaaacaa tattattttt agacatctcc
    17521 ttttctaatt aataatataa aattttacat caattattct acatacagtt tagaccaatc
    17581 ttcaaataat attgcaggag atggtttagc atcttttatt ccttttagat ttttttgagc
    17641 tgccattaaa ttttgttcat aatctatagt gtaaataggc gcatccttag atatatcctg
    17701 ttgtatttgc ttatatattt cttcacgttt ttccttgttt aactcagtaa atcctttttc
    17761 ccataaagca tctatttcag cattagaata tttagtttgg ttgcaatatt gaccagtttc
    17821 aaacatacct ctatatgggt ttggttctaa tccccaagca taaccattta catacatatc
    17881 acattcagtg ctattactaa atagtatatt gaagaatgcc ttactttcat aaggaactat
    17941 ttcagcttct ataccaattt ttttcaactc ttgttgagct actaaggcat agtttttatg
    18001 cccaaatcta cctgtgttat aacctatttt taatttatca attttaacac cacttttatc
    18061 caataagtct ttagccttat ttgtatcttg gtcatatcct tcaacacctt cttctgtata
    18121 gaagtctgct tctggaacta atatagattt agcaggtaca gaaccttctt ttccatatgc
    18181 tgattctatc atttcatttc tatttaaggc atatgataag gcttgtctta cttctttgtt
    18241 agccatgttt gatatatttt ggttaaatac aatgtagttt aatctttctt cactataagt
    18301 ataagtttgt aagtttgagt ttgattttgc cttttcatat ccttcagcag atgtcttcat
    18361 aagtgaaatt tcgccattat ttaaagctgc ttcttgagat gcttcattag gaattatttt
    18421 tagcgctata gaatctgcct taggttctcc accaaaataa tctgcattct tttcaaatac
    18481 tatgctttca ccttttttcc attccttaaa cttgaatgca ccagaaccaa ctggattatt
    18541 atttttttct gacttagcta tatttgattc tccttcaaac acatgttttg gtattggaga
    18601 aattttagat ataccatata agaaagattc acttgcagtt ggtaaagtaa tcttaactgt
    18661 taaatcatca attttttcta ctttaactgg tttctcaccc accaataagt attgtcttga
    18721 tggtgaattt tgtttttcgt ctaaaactgt attaaatgta aataagacat cttcagcagt
    18781 tattggcttt ccatcatgcc attttaaatt ggattttaat tttatagtta attccttact
    18841 atctttaaaa tctacttttt ctgctaaata atttactaaa tttccttttt cataagtata
    18901 aagaggagca taaactatat ttgatgcaag agagtcttct ttaacgtttg caaaagctcc
    18961 attgattata tctgggtcac ttgccatagg tattacaaca gttccaccag atttgcttcc
    19021 actagacttg tctttatcat tggaagcaca ggctgtaaat gtaagcatta atgatgaaac
    19081 taagattaat gatgccaatt ttttaaattt catattctta taaaacctcc ataaaataat
    19141 atttaacaca tattttttat tgtataatag taatcattat taaatcaata tatatagaaa
    19201 ttaactatta tattattata attaatagaa aaaaataata aaatgtaaga aaatgttgta
    19261 atttaaattt ttataaaata caatcaaaag gtgtatgtta tcttaagtaa ttttgctgag
    19321 aggagaaaat aatgagtaaa gcactaattg aggttaaaga tttaaaagtt tattttcata
    19381 cagataaagg gattgtaaag agtgtaaatg aagtaagttt taatataaat gaaggtgaaa
    19441 ctattggaat agttggagaa tcaggatgtg gtaagtctgt aacagccatg tctttgatga
    19501 agttacttcc aacatcaaaa atagaaggtg gagaaataat ctttcgaggc aaagacattt
    19561 taaagatgaa tgaagatgaa cttatgggaa taagagggaa tgagatatca atgatttttc
    19621 aagaaccaat gacatcttta aacccagcgt ttactgttgg aagtcaaatc attgaaggta
    19681 ttatgataca tcaagattta tcaaaggaag aagctaagaa aaaggtaatc gatatgataa
    19741 aactggtaga aataccaaga gctgaagaaa tatataattc atatccacat gaattatcag
    19801 gtggtatgag acaacgaata atgatagcta tggctttatc atgtaatcca aaattactta
    19861 tagctgatga accaacaaca gctttagatg ttactattca agctcagatt ttagatataa
    19921 tgaaaaatat aaaagaaaaa ttaaacacat ctataatgat gattacacat gatttgggag
    19981 tagttgcaga aatgtgtgac aaagtattag tgatgtatgc tggcaaaatt atagaagtgg
    20041 cagaggttgt tgaattattt aaaaatccaa aacatcctta tactattgga cttctaaaat
    20101 caaagcctgc tcttggaaaa aacaaagata aaagattgta ttcgattcca ggacaagtac
    20161 caaatccaat tggaatgcca gattcatgtt attttagtga tagatgtgaa aaagtatgtg
    20221 ataaatgtag aactcagata cctcctttaa tagaattgaa tagtggtcat agtattgcat
    20281 gttggttata taaaaaggag gagatataat tgaaaaaaga agagagttta ttaaaagtag
    20341 aaggtcttaa aaagtacttt gaagttaaat ctggattatt tgataagaaa attaaatctg
    20401 taaaggcagt agatggaatt tcttttgatt taaaaaaagg agaaactttg gctatagttg
    20461 gagaatcggg atgtggaaaa tcaactgcag gaagaaccat tcttagatta attgaaaaaa
    20521 ctgaaggtaa agttgaattt aatggtgtag atatatataa gttagagaag gaagaactta
    20581 gaaagttaag acccaaaatg caaatgatat ttcaagaccc atatagttca ttaaatccaa
    20641 gattaaatgt ggaacaaata atatccgagg cagttataga acataattta gtgccaaaga
    20701 atgaaataaa agataatata ttaaatgttg tagaaagttg tggtctttct aagtaccatt
    20761 taaatagaaa accaggtgag ttttctggag gtcaaagaca gagaataggt atagcaagag
    20821 cattagcatt aaatccagaa tttatagttt gtgatgaacc tgtttctgca ctagatgttt
    20881 ctattcaatc tcaaattata aatttactta tggatttaca ggataaatat aaactttcat
    20941 atttatttat atctcatgat ttgagtgtag taaatcatat tgcacataga gtttgtgtta
    21001 tgtatctagg ttctataata gaaattgcaa ctaaaggaga actttactta cattctcaac
    21061 atccatatac aaaagcactt atgagcgcag taccaataca agaccctaca attaagaaaa
    21121 atagaattat attaaaagga gacataccat ctccaacaaa tccaccaagt ggatgtaagt
    21181 ttcatacaag atgcccttat tgtaaggaaa tatgcaaaaa agagattcca gttttaaaag
    21241 aaatatcaag aaatcatttt gttgcatgtc atctagtata gatatttcaa aaattaaatt
    21301 tgatgtatat aataagaata atagaataaa ctgaaaattt ttttgattaa gatgggattt
    21361 catgctgtta atattaagtt agttgatata taagtttaat ctataatatt attattgaaa
    21421 agagaggtag ttatgtcaat tttagtaaca ttgttgggat tattggtttg tacaacgatt
    21481 tcaactgtat ttagcaaaaa gtggagtaat attcctcttg caatatatca aattgtacta
    21541 gggattatat tgtctatttt gccatttaaa ttctcatttt catttaatcc agaaatattt
    21601 gtaatatgta tcatagctcc attactattc agtgaaggac aaaatgtatc gagaaaagag
    21661 ctattagagc ttagaaaacc aattttgctt cttgcatttg gtttagtttt aataaccgtg
    21721 tttgcaggtg gaatctttat acattttctt attcctggga tgccattatc tgtttcattt
    21781 gctttggcag cagtaatatc tccaactgat ttagtagcag taaaatcaat tacacaagga
    21841 ttaaattttc ctaaaaatat aatgagtata cttgaaggtg aatctttatt aaatgatgct
    21901 gctggagtag tagcatttaa ggttgcagta cttgctacag ttacaggagt tttttcaata
    21961 gaagaggcag ggattcaatt tatgattaca gcttttggag gaataatagt aggaagtatt
    22021 ttaggataca taattataaa aataagactt agtttacaca agtggaattt ggaagaaatt
    22081 ccaatggtaa tagttataca aataatgact ccactttttg tatattttgt agcagaagaa
    22141 ataggagtat ctggaatcct agctgttgta atggcaggga tagctcatgg aattgaaaaa
    22201 gagcatttac aaaatacaac tactaaatta aggataatat cagacaatac ttggtatgtt
    22261 ttggagtatg tattgaatgg atttgtcttt acactgcttg gttttttgtt accaagtata
    22321 tatacaggac ttagtagtaa aaatgaaaat atggcattgg aattgacttt tatttctact
    22381 ttaattgtac ttattttatt tgttataaga tttatatggg tatatttatg gcataattct
    22441 tttataaaac ctaaaaagaa tcctttaaat aatttttttg taggtttttt aggttttaag
    22501 gatgaagaag ttataaaaga gagtatttct aaatgtaaat actcactaat agttgctact
    22561 tgtggtgttc atggtacatt tacactagca actgccttat ctataccttt ttatttggct
    22621 gataaagatg tatttccaat gagagatact gttttattta tatcatcaga agttattctt
    22681 ataagtttag tattggcaac aattttactg ccaagattat taaaaaataa cgtacaagag
    22741 tcagaacctt tattatctga taaagaggca tataaactaa ttttgaaaga agccatttta
    22801 aaacttagta atgaaaaagc acaagaagta caaccagtta tacatgattt aaatgaacag
    22861 ttggtagact cagaaaaagg aatactaaat actccagata ataaacttat aagtatgatg
    22921 attgcagaag cagaccaaca gggattatta gctgttttga aactattaga agatggtaaa
    22981 atttctgata gagcctttaa attgtataga ttttatatta caaaatcaag aaaatctgtt
    23041 caaaaatcta tatttaaaat tgtaaaatta aaaataagca tgtggattat aaatagaaaa
    23101 gtaaagaaag aagtaaaaga aaaaattaca aaaatagtat ctgagaataa agaaataatc
    23161 aaagagtttc accatgcata tgcactttct actaaaactg ctatatcatt tataaataaa
    23221 aatactgatg aagataacag acatgaggcc ttaatagcaa tcagattcta taatcgttat
    23281 ttgaattgga ttactagaaa gattcaaaat gatgataagt ttgaggcaag agtagaatac
    23341 tacagagtaa tggcgataca aagtcaaaga gacagtgttc aacaattagt tgaaactaag
    23401 caaatatcaa gagaagtggc taaagagtta ttggaaaata ttctctatga tgaaatgatg
    23461 atgatagaat cataaatata atggaataaa atttgatttt tgatgttaaa attaatacta
    23521 aaaaatagga aatagttttc aataatttac ttgacatata tatttattta taatagaata
    23581 aaaccataaa ttgaatacaa tatataagca ttaacaagct ggattgttac tggtaagcag
    23641 gcaataccta ggttaaggta tacaggtgaa ttgtatctta atttaggtat ttttttatga
    23701 ataagggaaa ttgtattttg ggagagatat tgattatgat tattcaacaa atttataata
    23761 ataatgttgt attagtttta gatgaaaata taaaaaaaga attaatatta acaggatgtg
    23821 gaattgggtt tcaaaaaaag aaaggtcaag aaatagataa aagtaaaatt gaaagaactt
    23881 ttgtaataca agatgaaagc tttttagata aaattagtaa actagcaagc caggttgatg
    23941 agaaattttt tgaggttagt actgaaataa ttgcttatgc agaggagaac ttgaatacta
    24001 agctttatga atatatttat gtagcactta ctgaccatat tgcatttgca attaaaagat
    24061 atcatgaaaa cataactata aagaatgatt tactacatga gataaaaaga atacataaaa
    24121 aagaatatga gattgggaaa tgggctgtag attatataaa taaagaattt gatgtgaaat
    24181 ttccagtaga tgaagctggt tttatagcaa tgcatatagt aaattcaaac tataagggaa
    24241 gttcaaagga atcactatta attactaaaa ttgtaaaaga catactaaat attataagat
    24301 attattatag agttgaattt aaagaagatg atataaatta tgataggctt ctgactcatt
    24361 taaaattctt tgcaaaacga ttagtaaaaa aagaaaaaat caatgataca aataatgaaa
    24421 ttatagatat aattaaagta aagtatgaaa aggattatga ttgtgcatat aaaataaaaa
    24481 ctcatgtaga aaagaattat gattactatg taagtcaaga tgaactactt tatttaacat
    24541 tacatatcaa aagagttata tctgtactga atttataata aagagtagtt taatacagaa
    24601 tgtatagatt taatatatta tgtttaattt taatatatta tttaaaattt aatataaaat
    24661 aataatttta aaacattaag attgttactg ataatgcagg caagacttat gtttataaaa
    24721 aaattataat agtttatttt taattctgaa attaggttaa tataggttta tttatattaa
    24781 cataagttta attttagaat taagtatact aatgtaactt tttataaact taagtctttt
    24841 ttatttaaaa aaatataaaa taataaggag agaaaagtaa atgaaaaaag tatttggtgt
    24901 tttacaaaaa gtaggaaaat ctctaatgct accagttgca ttacttcctg cagcaggtat
    24961 attacttggc gtgagtaatg ctttagcagg agacgcattg ttggctcatt ttccagcttt
    25021 ggcgaatcct acatttcaac ttataataag tataatggaa agttcaggtc aaattatatt
    25081 taataactta ccacttatat ttgcagttgg ggtagctgtt ggtttaactg aaggagaagg
    25141 tgttgcagca ttggcagcca tagtaggttt tttaattctc aatacatcta tgggtgttat
    25201 ggcaggtgtt acagaagata tggtagacaa ttctatgtat gcaaatattg ttggaatacc
    25261 aacaattcaa acaggggtat ttggtggcat tattataggg atagtggctg cttatcttta
    25321 taaaaagttt tataaaattg aattacctca gtatctaggt ttttttgcag gtaaaagatt
    25381 tgtaccaatc gttacttcat tagttgctat agcaatagga attatattgt cttttatttg
    25441 gcctccaata caagagggat tacttagttt ctcaaaaaat gttatagaag caaatcaaac
    25501 actagcagca tttatggaag gttctttgga aagactatta ataccatttg gtttgcatca
    25561 tgttttccat aatccatttt ggtatcaatt tggagaatat gtaaataaag ctggtgagct
    25621 tgtaatgggt gaccaaaaga tattctttgc tcaacttaaa gatggagtaa atttcacagc
    25681 aggtactttt atgacaggtg catttccatt tatgatgttt ggtcttccag cagcagctct
    25741 tgctatggtg catgaagcaa agccagaaaa gaaaaaattt gtagcaggta taatggcatc
    25801 agcagcatta acctcttttt taacaggaat aaccgagcca atagagtttg cgtttttatt
    25861 tgttgcacca gtattatttg caatacattg tttattttca ggtttatcat acgcaattat
    25921 gcaattatta ggagttaaaa ttggattcac attttcagga ggattaatag atttcttatt
    25981 atttggtgtt cttcctaata gaactccatg gtacttggct atcttggtag gtattggttt
    26041 tgcagtatta tactatagtg tatttagatt tatgataaga aaatttaatt taaagacacc
    26101 aggaagagaa gatgagtcag aaactaacgc tgaagtcatt gaaatgtcta agaatgaatt
    26161 agcatcagag attttgattg ctttaggaaa taaagacaat ttaaagagcc tagatgcgtg
    26221 tataacaaga cttagattgg ttgtaaatga tataaataaa gtagataaag aaaaattgaa
    26281 atcacttgga gcagcaggtg ttatggttat tggcaacaat gttcaagcta tatttggacc
    26341 aaaatcggat tcaataaagt ctgatataaa agatataata gagggaaaaa caaaggttga
    26401 aagtactgtt actaaaaatg ttgataaaaa taaagtgaaa aaaaatatgg aagttgcaaa
    26461 tatggaaaat gctttagtag aaaagattga ttcattaaca aatggagaga tattagatat
    26521 agaagaagtg ccagattctg tattttcaag taaactgatg ggggatggat ttgcaattaa
    26581 atctagtgat ggattgattt attcaccagt tgatggaact attggagtta tatttccaac
    26641 taagcatgca ataataataa aatcccaaaa aagtggagta gaaatactta tacatttagg
    26701 gatagaaact gtaaatttag aaggaaatgg atttgaagta tttgtgaatg taggggatga
    26761 agtaaaagcg ggagaaaaat tagttaaaat ggacttagaa tatattgaaa aaaatggtct
    26821 atcaacaata agtcctgtag tatttacgaa tttagaacaa aatcagaaat taagcattaa
    26881 aaaaggagta agcacagcaa aagaggagaa tagagtaagt atattaagag atgatattga
    26941 aaaaatagga tagctatatg atagtgtaac taaaagttat gtgggattta ataaatatat
    27001 aaataggttc agttaacatg caatttattt aatatatatt ttaagtaata gaaatatata
    27061 tcattatatt ttgatatatt ttaaaacatt aaaaaacttg aggtattagt aagataaata
    27121 cactaatact tcaagtttta ttaaaataaa atttacataa attatctatt catctaaaag
    27181 agtaatatcc aaaggatttt tattgtttat ataattttta ggagataccc cgtagttttt
    27241 tttaaatgcc cttacaaaac tagaataatc gttgaatcca catttattac aaacttcact
    27301 cataaggtga ccattggtta ttagtgactt tgccaaaatt aatctttttt gaactatata
    27361 attgtgtata gacgttccag tatgagattt aaattttctc ataagataat acttacttat
    27421 aaaaaattca gacgatatgc tatctattga aagcttatca tctatatttg aatttatata
    27481 gtttaatact ttcttaatag tttcatcata aaaaacatct tctgttgatt ccatattttc
    27541 attttttagg aaaattctgt ttaagaatat cataagttgt ataaataatg catttctcaa
    27601 tatttcattt ccaaattcag tattggattc ggaaatattt atttgctcaa taaaactctt
    27661 aatagattca atatcatgca tatttaaacg caatctattg catttattct ctgtagctat
    27721 tttaaaacat ttttctaagt cactactagc atcactattt tttttcataa attctgggct
    27781 aatccatatt gcaattctct cataaaagac atttgaatct ataactggtt tatggatttc
    27841 attattgctg ataaagagta tatcccaagg ctttagttta tagggaatac catcaacaaa
    27901 ataagttaca tttccattta taaatataat tatcttatta aaatcatgat tatggtattc
    27961 aaattgtata tcttttttat ctctaatatt aaatatttta aagttatcgt ttaagtaccc
    28021 gattttatta tccatagttt ttatagaaga cctttcctca gttatattat agaaaattat
    28081 agagcatttt ttgcaatgtt tcaagcattt ttaaccattc acatagtaaa aaattcttaa
    28141 tataatatta tatgtagtgt aattttagca gaaagagttt aaagggagga aagcatttat
    28201 gaaattaaat gatattatac aggggttgga tataatcaat gtcaagggtg aattaaatat
    28261 agatattaat aatgtacaat atgattcaag aaaggttaca aaaggtacat tatttatatg
    28321 tataaaagga tttgtgtcag atggtcataa gtatataaaa gatgccatag aaaagggagc
    28381 aagtgcattt cttgttgaag aagatgtagc aataaaagga tgtacattta taaaggtaaa
    28441 agatacaaga aaagatatgg caaaagtagc tgataatttt tacaatcatc catctcaaaa
    28501 atttaacgtt ataggagtta caggtactaa tggaaaaact agtataacta ctatactaaa
    28561 tgagatatta actttaaata agaataaagt tggacttata ggaactataa aaatttttga
    28621 tggagaaaaa gacattgttt ccaattcaac aactccagaa agtatagatt tacaatatca
    28681 ttttaataat atgttggata atggatgtga ctactgtgcg atggaagttt catcacattc
    28741 attggcatta aatagagttg atgaaacaga ctttaaacta ggcatattta caaatttaac
    28801 accagaccat ttagattttc ataaagattt agaggcttat agaaaagcaa aagaaaaatt
    28861 attttttaag actactatgg caaatataat aaacatagat gatgaaggtg gaaaaaagat
    28921 atatgaaaat ataaaaggta taaatgtacc ttgttacact tatggtgttg atactaaggc
    28981 tgattttatg gctagagata taaaatcaga ttcggatgga gtatcttata gattaataac
    29041 accttcatat gaagaagtaa tatttatacc agttccagga atgtttacag tatataatac
    29101 tttagcagtt atagctgctt gctatgtact tggcatacct aaaccaatat ataaagaagg
    29161 ccttagactt tctaatggag tatcaggaag gtttgaaaca gttccaaatg ataaaggtat
    29221 aagcgtaatt gtagactatg ctcatactcc tgacgcactt gaaaatgtac taaagacaac
    29281 tcaacaattt gcagaaggaa aaataatttc tgtgtttggg tgtggtggag atagagatac
    29341 tgaaaagaga cctttgatgg gagcaattgg acaaaaatat tctgatttat gtattataac
    29401 ttctgataat ccaagaactg aagaaccaga ggcaataata aaagatatac ttgaaggaat
    29461 tgacaagaaa aaagaaaatt accatgttgt tgttgataga gaacaagcta tagcagaagc
    29521 tataagtatg gcaaaaaagg atgatgttgt aattataaca ggtaagggac atgaaactta
    29581 tcaaattata ggtaaagtta agcatcattt tgatgataaa gaagttgcaa atgagtgttt
    29641 aagtaagatg tagcataaaa attaaacaaa atacaagaac aaaaatgtaa tatcaagctt
    29701 cactttgata ttacattttt tttatatgta aataattgta aaatagataa tgcaataatg
    29761 taaacactaa tatctatatt aattaattta ttgttgtaat aaagtttata attttgtata
    29821 attacagtaa atatggaaaa ataagtatta ttataaaatg tttacaataa ttgtcataag
    29881 ataatgatta tacatacata gattcgagaa aggcaggaag cataatgtta agtgatgcaa
    29941 aaaataatag gtatggtttg tccataattg ctgtcataag tatcttagta attacaattt
    30001 cctgtttgat ttatggttca aggttgaata agacattagg tgaagaaacg aatcaatatc
    30061 tttcagaaat agcaaatcaa agcgtcaatg tgttaaaaaa acaaattaat ggagatataa
    30121 agttactaga gtcaatttca atatttattg aaggagaaga gtcttttgaa gtagataata
    30181 tcctttctat actaaaaagg caagctatta atagttcatt taaaagaatg ggagttattt
    30241 taccaaatgg tacagcatac actacagatg gttatataga agacttctct aaaagagatt
    30301 atttcttaaa atcaatgcaa ggagaagtgg ttataacagg aaaattaaaa gatgtaatag
    30361 aagatgataa aaataccaat agtaatatta atgtatattc tgtaccaatt tataaagata
    30421 atgaggttaa aggagttata tttgccacac atagtacgaa gttgtatgaa aaaatacttt
    30481 ctgtacctac atttaatgga aaaggatatt cttatataac tagaagtaat ggtgatattg
    30541 ttttaaatcc aagcagtaaa aacgccaact ctactaaaga aaatttattt tcttacattg
    30601 gcagaagtga ttcaatatca aaatctagta tcaacaatat gaagtcaaat ataaaaaact
    30661 ttaaatcagg ttcattaagt tatagtatgt ttggaaaagg atattattta agttatgccc
    30721 caataggaat caatgattgg tatctttttt ctgaagttcc taaaactgct atatctgaga
    30781 aatcatatgc tattataaaa cttacattat cagcatgtgt agtcttgatt ataatcttta
    30841 cggcattaac aatttatata ctatttatac ataaaaagag taaggaaaaa ttggaagaat
    30901 ttgcttttaa agataacgtt acaggtattg gcaattcaaa taaatttaat cttgaagggg
    30961 gaaaattttt atcctctcat gagaaaaaga atctagtttt aatttatttt gatatagata
    31021 agtttaagct agtaaatgat aggtttgggt atgaagaagg agatagagta ttaaaggaaa
    31081 tagcagagat aattaagaat atatttaaag agcaatctgt attttcaaga atttcaaatg
    31141 acaattttgc tataatattt gaaaagaaaa ataatagaga gtcaattata gaaatatgtg
    31201 aacttatacg taaaaaattg tcaatgataa aaacaagttt gggagtagaa ctcaatttaa
    31261 ttccatccat aggtgtatat tttgttgaag agggagaaac aaatatttct acctgtcttg
    31321 acaaagctat gattgcaaaa actacagtaa aaagaaaata tttaaaagtt tatgagattt
    31381 atgctgaaaa tttaaaagaa actctcatag aagaacgtga tattgaacaa gaaatgcatg
    31441 atgctttaaa aaatgaacaa tttaaagttt atttacagcc taaggttgat ttatcaacta
    31501 ctaaaattgt aggtgctgaa gcacttgtac gttggcaaca tcctaaaaaa ggtctaataa
    31561 gtccaggtgt atttattcct atttttgaga aaaatggctt tataacagaa ttggatatgt
    31621 ttgtatttac ccaagtttgt aaaaatttta aacgttggga aaatgaaaat ttccccacat
    31681 ttcctatatc aataaactta tcaagagtac atcttgagaa tccaggcttt atttctgagc
    31741 ttgaaagaat aacaaaagaa tatgaaattg aaccaaattt aattgagatt gaattgacag
    31801 aaagtgcaat atttgacaat acaaaaatat tatttaaaat aatgcaaact ttgaaatctg
    31861 ttggatttaa aatttctatg gatgattttg gttctggtta ttcttcttta aatatgctta
    31921 aagatatgcc aattgatgta ttgaaattag ataggcaatt ctttataaca gtgggaaatg
    31981 caaaaaagag tcaaattgta gtttcaagta ttgttcaaat ggcaaagcaa cttgatataa
    32041 aagttgtttc agaaggtgtt gaaactgtag agcaagcaga gtttttaagg tttataggtt
    32101 gtgatatggc tcaaggattt ttatttgcta gaccaatgcc aatagaagaa tatgaaaaat
    32161 taatattaga tagatatagt ttaaatagaa aagaaacaaa ttaaataaaa ttgtgaatta
    32221 taacataaaa atttataaaa aagtgaactt tgttaatcaa tgttcacttt tttatgtaaa
    32281 aatatactat tataaaaaca taaatcattg ttaggatatt attttaggaa ttattgggca
    32341 aagtttatta attatgtagt attttttcta tttgaatgtt tttgaggtaa gagtatatgg
    32401 gattatagca aaatac
//
LOCUS       DAMKMI010000031        32218 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.032, whole genome shotgun sequence.
ACCESSION   DAMKMI010000031 DAMKMI010000000
VERSION     DAMKMI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.032"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     regulatory      134..224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            633..950
                     /gene="flgB"
                     /locus_tag="PC429_001359"
     CDS             633..950
                     /gene="flgB"
                     /locus_tag="PC429_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF3846874.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            958..1362
                     /gene="flgC"
                     /locus_tag="PC429_001360"
     CDS             958..1362
                     /gene="flgC"
                     /locus_tag="PC429_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF3846875.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            1372..1689
                     /gene="fliE"
                     /locus_tag="PC429_001361"
     CDS             1372..1689
                     /gene="fliE"
                     /locus_tag="PC429_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF3846876.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            1714..3264
                     /gene="fliF"
                     /locus_tag="PC429_001362"
     CDS             1714..3264
                     /gene="fliF"
                     /locus_tag="PC429_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF3846877.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            3277..4347
                     /gene="fliG"
                     /locus_tag="PC429_001363"
     CDS             3277..4347
                     /gene="fliG"
                     /locus_tag="PC429_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF3846878.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            4340..5071
                     /locus_tag="PC429_001364"
     CDS             4340..5071
                     /locus_tag="PC429_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF3846879.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            5075..6394
                     /gene="fliI"
                     /locus_tag="PC429_001365"
     CDS             5075..6394
                     /gene="fliI"
                     /locus_tag="PC429_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF3846880.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            6425..6871
                     /gene="fliJ"
                     /locus_tag="PC429_001366"
     CDS             6425..6871
                     /gene="fliJ"
                     /locus_tag="PC429_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF3846881.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            6900..8105
                     /locus_tag="PC429_001367"
     CDS             6900..8105
                     /locus_tag="PC429_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF3846882.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            8123..8731
                     /locus_tag="PC429_001368"
     CDS             8123..8731
                     /locus_tag="PC429_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF3846883.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            8758..9741
                     /locus_tag="PC429_001369"
     CDS             8758..9741
                     /locus_tag="PC429_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF3846884.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            9754..9957
                     /locus_tag="PC429_001370"
     CDS             9754..9957
                     /locus_tag="PC429_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF3846885.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            9957..10775
                     /locus_tag="PC429_001371"
     CDS             9957..10775
                     /locus_tag="PC429_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF3846886.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            10765..11460
                     /locus_tag="PC429_001372"
     CDS             10765..11460
                     /locus_tag="PC429_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF3846887.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            11472..11915
                     /locus_tag="PC429_001373"
     CDS             11472..11915
                     /locus_tag="PC429_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF3846888.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIADLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            11930..12304
                     /locus_tag="PC429_001374"
     CDS             11930..12304
                     /locus_tag="PC429_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF3846889.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            12305..12970
                     /gene="fliP"
                     /locus_tag="PC429_001375"
     CDS             12305..12970
                     /gene="fliP"
                     /locus_tag="PC429_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDF3846890.1"
                     /translation="MELLSKLPLADVPYTSSMQLFLFLTALMFLPFVMFMMTSFVRIV
                     ISLSFLKSALGAQQAIPSQILVGLAIVLTIFIMRPVLNEINEKALQPYMKEEVTMEEA
                     MKEAEGPIKEFLLTQTRQTDLDLFVEQAGLKEKKLTRENIPLSVVVPAFAISELKTAF
                     QIGFLIYIPFLIIDLVVASVLMSMGMFMLPPVMISLPFKLLLFVMVDGWNLIVKTLIL
                     GFG"
     gene            12983..13252
                     /gene="fliQ"
                     /locus_tag="PC429_001376"
     CDS             12983..13252
                     /gene="fliQ"
                     /locus_tag="PC429_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF3846891.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            13264..15078
                     /locus_tag="PC429_001377"
     CDS             13264..15078
                     /locus_tag="PC429_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF3846892.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNNIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            15087..17162
                     /gene="flhA"
                     /locus_tag="PC429_001378"
     CDS             15087..17162
                     /gene="flhA"
                     /locus_tag="PC429_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF3846893.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            17137..18153
                     /gene="flhF"
                     /locus_tag="PC429_001379"
     CDS             17137..18153
                     /gene="flhF"
                     /locus_tag="PC429_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF3846894.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            18150..19028
                     /locus_tag="PC429_001380"
     CDS             18150..19028
                     /locus_tag="PC429_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF3846895.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            19042..19743
                     /locus_tag="PC429_001381"
     CDS             19042..19743
                     /locus_tag="PC429_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF3846896.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            19759..20181
                     /locus_tag="PC429_001382"
     CDS             19759..20181
                     /locus_tag="PC429_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF3846897.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            20166..20351
                     /locus_tag="PC429_001383"
     CDS             20166..20351
                     /locus_tag="PC429_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF3846898.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            20392..21165
                     /gene="flgG"
                     /locus_tag="PC429_001384"
     CDS             20392..21165
                     /gene="flgG"
                     /locus_tag="PC429_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF3846899.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            21186..21947
                     /gene="flgG"
                     /locus_tag="PC429_001385"
     CDS             21186..21947
                     /gene="flgG"
                     /locus_tag="PC429_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF3846900.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            21970..22851
                     /locus_tag="PC429_001386"
     CDS             21970..22851
                     /locus_tag="PC429_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF3846901.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            22854..23228
                     /locus_tag="PC429_001387"
     CDS             22854..23228
                     /locus_tag="PC429_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF3846902.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            23260..24663
                     /locus_tag="PC429_001388"
     CDS             23260..24663
                     /locus_tag="PC429_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3846903.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            24779..26716
                     /gene="htpG"
                     /locus_tag="PC429_001389"
     CDS             24779..26716
                     /gene="htpG"
                     /locus_tag="PC429_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF3846904.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            27103..28233
                     /locus_tag="PC429_001390"
     CDS             27103..28233
                     /locus_tag="PC429_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF3846905.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
     gene            28460..29482
                     /gene="splB"
                     /locus_tag="PC429_001391"
     CDS             28460..29482
                     /gene="splB"
                     /locus_tag="PC429_001391"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF3846906.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
     gene            29664..30575
                     /locus_tag="PC429_001392"
     CDS             29664..30575
                     /locus_tag="PC429_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3846907.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            30590..31006
                     /locus_tag="PC429_001393"
     CDS             30590..31006
                     /locus_tag="PC429_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF3846908.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(31106..31498)
                     /locus_tag="PC429_001394"
     CDS             complement(31106..31498)
                     /locus_tag="PC429_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3846909.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            31639..32034
                     /locus_tag="PC429_001395"
     CDS             31639..32034
                     /locus_tag="PC429_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF3846910.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT        13209 a         3421 c         5913 g         9675 t
ORIGIN      
        1 tttattataa aattgtaaac tatagtgttt ataatctttg taattattgg taattaaatt
       61 taaaaaataa aatatttaat tatacacaaa aaagatattt aaaaaatttg ttatttattg
      121 tatacttaag ttaaaactaa ataggcaaat ctagagaaat ctagtgacgc aaagctatag
      181 ggactaaggt ttatatacat aaactatgtc agccagttgc caaaaagagt cctaggtgta
      241 ttgtatacct aagaaaagtc tataatgact gcctttttgg cagtcatttt gttatggaaa
      301 aatcacttta tttcagtaga aaatacacat aataacatat tttaccaatt ataacataaa
      361 ataaagaaaa attatctcaa aaagttacta ttttacaaaa aagagcaact tttcgaagaa
      421 atatttaaat acatattaaa atttttttat attttcatgg gacttcatta aaaaaatatt
      481 agttttctta ctaaattgat acattattgt attaatgtaa tggaaatgcg attaatttaa
      541 ataaataaag aagtcatttt ttgtaatata gcaacttcat tttttttatt aataaacaaa
      601 atagataaaa tatattttga ggaggggtga gtgtgaatac atatgacatt ataaaaatgg
      661 gattagatgc aactaatcta agaagtcaga caatagcaaa taatatagca aatataaata
      721 ctgaaggata taaaagacat tatataactt ttgaagaaac actaaatgat gctatgccta
      781 aaatagaaga aaaaacagac aattctaaag gtttaagagt agatggtaat aatgtagaca
      841 tagaagaaga aaaagtaaat caagcaatgg caacattaca atataatgct ttgataagtt
      901 ttgcaaatgg aaaaatagca atgaccaaat caataataag tgggaggtaa ttagaagatg
      961 agtgtgttca gtggaatgag aatatctgct agtggattaa cagcagaaag tttgaaaatg
     1021 gatatcatat catccaatgt agctaatata aatgctacaa gaggtccaaa tggagaagca
     1081 tataggagaa aagttgtttc atttgaagaa aactatgata ataaattagg tatgctagga
     1141 gtaaaagtta catctgttga agatgataaa tcacctctta aagaagtata tgacccatct
     1201 catccagatg caaagccaaa tggatatgta gaatatccaa atgtaaatat attggtagaa
     1261 atgacagatt taataacagc ttcacgagca tatgattcta acgtagatac attgaatgct
     1321 cagaagtcta tgatatcaaa agctttggaa ataggaaagt agggataaat tatgccaatt
     1381 acacaaatta acaatttaat gcaaggtgca aagttagata atttgattgg agaagcaaag
     1441 ccattaactc ttaataataa agaagaaaaa aagaattttg aagatgtaat aatagatact
     1501 atagataaag ttaattttca acaagtaaca gcagatgata tgaaagaaaa atttataaaa
     1561 ggcgaagatg tttctatgca tgaagttatg ttaataggtc aagaagcaca actatctatg
     1621 caatatttaa tagaagttag aaataagatt catgatgcat atcaagaaat gaataaggtg
     1681 caaatttagc cttttgaaca ggagtaataa tcaatgataa ccagttttaa aaataagatt
     1741 aaggagcaag ttgctaaatc taggacaaag ataagttctt taaaaaaggg aactaaaata
     1801 gcattaggta taggggttat tgctatatgt ttagcgctag tatttacagc aatatctgcg
     1861 agcaaaaata agtatgcagt cttattttca gaactagatg ctcaagatgc tgcagatata
     1921 tcagcagagc tagagtctaa aaagatagat actaaggtaa aaggaagtac gatttatgta
     1981 cctagaacag aagttgataa gttaaggctg gagttagcgc caagtcttac aggtggttct
     2041 aaaggatttg cactaatgga tgatagttca tcaatagggc ttactgatga agaatttcag
     2101 attaagaaac aaagaatgat acaaggtgaa atagaaaaaa caataaagag ttttccacaa
     2161 gtggaagaca ctagagtaca tataacattt ggagagtcat cagtatttga gaaggaggct
     2221 gttccaggga gtgcagcagt ttatttgatg ctaaaaccag ggacgacttt agaaaaaaac
     2281 caaataaagt ctgttatggc acttatatca ggaagcgtta caaatatacc agagaaaaat
     2341 gttcaggtta ttgataacaa aatgaattta ttatctgaag gtattttgga ggatgaaaat
     2401 gttgatgaaa ataatcaacc aataaatagt ggcattagtg ttgataaaaa taaggcatct
     2461 gaacaagcat tgaataaaca actagaaaag tctattttag aaatgctaga accgatattt
     2521 ggtaagggaa aggtgaaagc tacagtaaat gctgatttaa gttttgatac agctgaaaaa
     2581 actgagataa aaattgaccc tgataaagtt gcaataaagg agacaaggtc gaaaaattca
     2641 tctaaatctg ctgcaaataa tgttcaaggt gtagatgcta acatgaataa ccaaggaaat
     2701 aacaatggta ctaatacaga agatagttta gatgagagtt ttgagtatga aacaggtaag
     2761 attgaaactc acactatagt tgcgcctgga gaacttaata gattgacagc ttctgttgca
     2821 atagatggaa aaatagctaa aggtgttcaa gctgatgtag aggatattgt taataatgct
     2881 ataggtatgg atggagcgag gggagatact cttagcgttg tttcaatggt atttgaccca
     2941 gaaggtaaga aagaggctaa acaagagctg gaagagatta aaaaagaagg atttaaaaag
     3001 aacataataa aagcagttat tggagtagta gcagtaatag cagttggagc tattggatac
     3061 ttaatatata agaaaagaag tgaacgagaa gatatggatt atgaagatga agattatatt
     3121 tctaaatctg attctgttga taggaaggat aatcctctat taggagatga tccagaactg
     3181 acattagagg aagctgttaa aatgtatgca gaagaaaagc cagaccaagt aacagagata
     3241 ataaagactt ggttaaatga gtaaggggga tagaaagtgt ccacggaaat tacaaatgtt
     3301 aatgcgtttg acttaagtga tataaatgaa agtgataaga atgagaaggt agaaggtgag
     3361 ggtttaaaaa aagctgctgt attattaatg acattaggcc caaatgtgtc tagtgatata
     3421 ataaaaagct tgcctgataa acaagttcaa aaaataggtt tggaaattgc aaatatttct
     3481 tcaatagctt caaaagaaag aagagaaatc ttaaaagagt ttgtagaaat aaatagggca
     3541 aaagattatg ttatagaagg tgggcttgaa tatgcaagag agttattaag taatgcttta
     3601 ggaaatgtta aagcaaacaa attacttgaa ggtatatcaa taaatacaag tacaaagcct
     3661 tttaaacata ttataaaact agatgtaaag aagatattag aaatacttag gaatgaaagt
     3721 gcacaaacta tagccgtttt acttgcacat atggaaccag aaaatggagc agaagtttta
     3781 tcatacttag attttgattt acaaaatgaa gtagctatga gaataagctc aatgggttca
     3841 atatctccag aagtaataaa ggtaattgaa agttcattag aagataaatt acattcaata
     3901 agtggtgatg attttaatga ttatggaggg gtgtctacct tagttcaaat attgattaat
     3961 tctgatagaa aaacagaaaa gaatattttg ggaactattg aagaacaaaa cccggattta
     4021 gcagctcaaa ttaaatctca tatatttgtc tttgaagata ttgtcaagtt agatgatata
     4081 agcatacaaa aagtgttaaa agaagtgact atgaaagatt tagtatatgc cttaaaaggt
     4141 gtagatgatg agatttcaga tgctatatat agaaatcaat cttctagagc agcagatgca
     4201 cttaaagaag aaatggaaat ggttggtgtt ataaaggtta cacaagttga ggcttctcaa
     4261 caaaagatag ctaatatcgt aagaaaactt gaaagagaag gtgccataac tattgtaagg
     4321 aattttggtg gtgactttgt tgaataataa gattattaaa agtaaaaaag cattatataa
     4381 aggagtgcta gatttaacca taatagataa agttagagtg ggagaatcaa atgtaaataa
     4441 agaaaatgat tgtagattta gggatgaggt aataaaagaa gctatagaat ctgctgagat
     4501 aaaaaagaat gagatattag aagaagcaaa aataaatgca caaaatatag aaaagcaagc
     4561 atatgaaaaa gggtatagtc aaggtcaaaa aaatggatat gaggatggat ataaagagtc
     4621 atatgatgaa tatacccaaa aagctaaaga tgaagcagat tttataaaaa atcaggcaaa
     4681 tttaatctta ttacaagcta atgagaagat gactgagtac ataaaagaaa aaaataaaga
     4741 gattgtaaga cttgctatta atatggccag ttctgtttta aaaaagcatt tcgaagatga
     4801 agaatctatg agtgatctgt taaagcaaat tattcaagat tatgaaggaa gtagttcatt
     4861 tatagtaaga tgtaattctt tctataaaga taatatagag aaagagtttg aattattcaa
     4921 agaggaaaca tcactaaaat caagtgtatt tgttattgca gatgatagta tagataaagg
     4981 caatgctatt atccaaaagg aaaatggaag aataatagtt ggtattgatt gtgctatgga
     5041 aaaactaaaa gaagaattgc ttggtgaata aagtatgtat aaaactgata tatctataga
     5101 ttttgacaaa ataaataaga aaatagaaaa tttagaactc actatatcag aaggtagagt
     5161 aaagaagata ataggactta ctgtagaagt tgaaggaata aaagcttttg ttggggagtt
     5221 atgcgttata tacaaccaag ttaataaacc agtgaattgt gaagtagtag gttttaaaga
     5281 taaagaagtc atattaatgg cgttgggaga attaacattg atagcacctg gttgtaaagt
     5341 tatttctaag gggatacctt taagtgttat gtgtagtgat aatttactag gtaaagtact
     5401 tgatggtctt ggaaatccta tagacaactc agatgctgtt ttaggagata gatacaacct
     5461 gaacaatgaa cctcctgatc caatgaagag aaaaaagata cgaaatataa tggaaacagg
     5521 tgtaagagca atagatgcat ttactacttg tggtgaagga cagagaatcg gcatatttgc
     5581 aggaagtgga gtaggtaaga gtacgactct aggtatgatt gctaggaatg caaaagcaga
     5641 tgtaaatgta attgctctta ttggagaaag aggaagagaa gtactagatt ttattgataa
     5701 agatttaggt gaagagggaa tgaaaaaatc tgttgttgtg tgtgcaacat cagataaagc
     5761 ccctctagtt aggttaaaag gtgctcttac tgctactgca atagcagaat attttagaga
     5821 ccaaggcaaa aaagtaatac ttatgatgga ttcagtaact agatttgcta tggcacaaag
     5881 agaagttggt gtagcaatag gagagccacc agctcaaaaa ggatatacac catctgtatt
     5941 tgcaatgttg ccaaaactta tggagagaac agggacatca gataaaggtt ctataactgc
     6001 attttataca gtattagttg atggagatga ctttaatgag ccaatagcag atacaacaag
     6061 agggatactt gatggtcata tagttttatc tagagacttg gcaaataaga atcactatcc
     6121 ttctatagat gtattaaaca gtttaagtag gcttatgaat gaaattgctt caaaagaaga
     6181 cataaaaata gcgtcttttg caagagacat gcttgctgag tatagagaag cagaagattt
     6241 gataaatata ggtgcatatg cttcaggtac aaataaaaaa atagatgaag caatctatta
     6301 tcatgagcat attattaatt ttttaaaaca agggataaat gaaaagagtt cttttaatga
     6361 aactataagt agtttgagaa gagtttttga ataaacaatt aaaaaaatat ttgaataggt
     6421 tgtgatggaa ttggataaaa actttaagtt tagattacag aaagttttag acttaaaagt
     6481 gaaagatgaa gaagagataa aaatggaatt tgctaagatt caacaaaaaa aaatagatat
     6541 agagactaat ttagaaaact tagaaagtaa ttatagcaaa tattctataa gtaaaaataa
     6601 cgatagtatt caaaatcaaa aaataactat taactattta ctagctttaa acaattctat
     6661 aatggattta tcagaagagt tggataagtc aacaaatgaa cttgaaaaag ctagaaagca
     6721 gcttataagc aaacagatag agagaaaatc attagaaaaa ttaaaagaaa aaaagtatgg
     6781 acagtattat aaagaagaaa atttaaaaga acaaaacact aatgatgagt ttgcaagtat
     6841 gagttattta aggaatagac aagttctata agaatagtag gaagtgagga tattattgta
     6901 tgaagataca agcagattct tataatatta ttaaagatat tggggataga aaaagttcat
     6961 ctaagaacaa aaatataaaa aataataaat ttgaagataa tctatataag gcaacagacc
     7021 ataaaaaagt taaagataga agggaagtaa gtaagcaaaa tattagctca gataaaccgg
     7081 aaaatagtga taatgagtgt catttagatg aagataaaag taatcactta gaagatgaaa
     7141 atacatatga aaaagattgt agtaagaata atgaagcttt gcagcaatta ctgaatcttt
     7201 taaaggataa ttataaaaaa gataaagata taaacactat attgaataat atagaaaaac
     7261 taagtatatc agatgaatta aaacaagaac ttaagcttaa taaaaatata ttaaataaga
     7321 ttgcaaatca aaattcagag ctaaataaag ctaatataaa taactctaat gatatagaaa
     7381 atttagacat agttaaatta agagaacttt tggaagataa tgctaataaa gatattgaag
     7441 taattactaa aactacagaa aatgatacca aaaatataga tttgtatagt tttgatagca
     7501 atagaatgga taatcttaac tcaaataaac agaaagatga tagctctgat attttggaaa
     7561 aactagcagg ggtaaatgga aataagagtg ggaattttgg acaagtaata aataaaaatg
     7621 ttgatatgaa taaaattaaa aatgataatg tcaatttaaa acagatagaa tcaaatgtaa
     7681 tggatgactc tataaaagct attaagcata tgaaaacaaa tgatatacaa gaattgacaa
     7741 ttaaattaag accaaaagaa ttaggagata tgaatataca attgttaaaa gatggtgaaa
     7801 gtatgagagc tgttgtgact gttttcaata aagatgtctt tgactctata aataaaaaca
     7861 tagcagattt aaagcaacat cttgaactta ctaatgtaaa tataaaagat gtatcagtgc
     7921 aaatgcacag cgataataca aatacatctg atacctttga taaagcattt gaacaacaga
     7981 atagacaaaa taaccaagaa aacatgagta agaagaatac aaatgataag cataataatg
     8041 tagtcataga agatgacatt aaagaaaata ctattgatga tgatagagta gatttactag
     8101 cttagtaagg gggaagtatt gcatgtacgg aatagcaacg gctttggcag gaactcaggg
     8161 aaactatagg tcaaataact taagccaact tgaaagaaat attaatgata ttgccaataa
     8221 acaagttaga agcaaaggaa atcagacagt aacagacaat gggactccaa ttataggaca
     8281 aggtgatgat gctgataaag acttgtttat gaagatactt gtggcacaga tgggtaatca
     8341 agacccaatg aatccgcaag acccaacaga atatgtgtca caattagcac agtttgcaac
     8401 tttagaaaag atgtcagcta tgaatgagaa gctagatgta cttttagcct taagtaattc
     8461 cactctaata aactctgctt taagtatggc aacatcatta attggtaaag aagtagaagt
     8521 atctggggaa gagaaagaag atgaaaacat tgtaggagta gtaaagtcca catatataaa
     8581 agatggggaa gtacacttag aattagaagt agatggaagt gacaaaactg taggtgttaa
     8641 atatgaaaaa ttacttaaaa taggagaggt agatgaaaat tcaggagaag aaaatgattc
     8701 ttcagaacac gaatctccag aagaagcata atattaaatg agaaaagagg taaattcatg
     8761 ataaaagcaa tgtattcagg tgtaagtgga atgaaagcaa atcaaacaaa attagatgta
     8821 ataggtaaca atgtagccaa cgctggaact acttctttta agaaaagttc agctagaata
     8881 acagattcat tttatcaaac tgtactatat gcaagtgctc caacagctgc attaggagga
     8941 acaaacttag gacaagtagg tgtaggtagt aagatatcaa gtataaataa agatatggta
     9001 caaggaaatg tacaaccaac taatagacca tctgatttaa tgatagatgg agatggatat
     9061 cttccagtag taagacaagg aacagttatg tatacgagag atggttcttt taatttagat
     9121 acaggattta aagcagctga tggtgctttg aagaatatta aaggtggaag acttgtaaat
     9181 ggtgatggat atatgttaca aggtatgatt tatgatgggg aatataaagc tgatactgga
     9241 gaatttgtta aaaagggtga tgctaaagga aaagagccta ttgtaatacc acttcaaagt
     9301 ataagtccta aggatggaac agttcaaaat gtactagagt ataatatatc aaaggatgga
     9361 acagtagaat ttttattgag tgatggtcaa agaactcaat ttgtctatga tatacctgat
     9421 ggtgctaagg taggagatac acctgctgga atagataaat taaaaggtac aatacaaaaa
     9481 gtgcaaacat atgcattcca aaatccagca ggactagatg cagaaggagg aaacttgttt
     9541 aaaccatcag caaactcagg agacccacaa atagcaggag ctacaggcac aataatacaa
     9601 ggggcaatag aagcttccaa tgtagatata gcagaagaat ttacagaaat gataatagca
     9661 agtaggtctt tccaagcaaa cagtaaaata atatcgacat cagacgagat acttcaagaa
     9721 ataataaatc ttaagagata gtaggtacat attatgataa gagtaacgga tttaaacgga
     9781 agaaaataca tattaaattc agaccttata gtgaggattg attctgtacc agaaagtagg
     9841 attctcctta caacaggaga aaaaaacctt gtaaaagagt cggtagatga gatagtagat
     9901 aaagtaataa agtataaaag aaaaataaat gtaggttata ttaggagcga aaaataatga
     9961 aaaaaacaga ttcactgaca tttattggac taatagtatc tacagtatta gttttagttg
    10021 gtgccgcaaa aggaagtagt tcagggttaa agaatttttt tgatgtttca tcaatattaa
    10081 tcacagtgct tggttcattt ggagctctaa tgattacatt tactatagat gatataaaac
    10141 ttataaaaaa tgcattacaa tactcattta agacaatgag tgtatcaaag ttagatttac
    10201 tagaacaatt taaaacactt tctaaaaaag ctagaaaaga aggtctttta tcaatagaga
    10261 gtgatgtaat gggaatagaa gatccattta tgaagaaagg gttagagctg tgtattgatg
    10321 gattggaaat caatgagata aggtccatct tagaactaga gatagattct gttgaagata
    10381 caaataacag atactctaaa ctatttaaaa cttggggaac ctttgcacct gcttttggga
    10441 tgttgggaac actaataggt ttaatacaaa tgttagctga ccttacatct ccagatttaa
    10501 tagcatctgg catgggaaag gcattaatta caacgtttta tggttcttta cttgccaata
    10561 tcgctttgaa cccaattgca tataatatag atgagaagac tgaaaaagaa atttatgtaa
    10621 aggaaatgat gttggaaggt ataataagca tacaatctgg ggagagcagt atagtagttg
    10681 aggaacgttt agccacatac ctttcaaaca atgagaagtt agaaattatg aaatccaata
    10741 aaaatacaga aagagcaatg agcaatggcg cgtaaaaaaa ataagaaaaa ggatgagata
    10801 aatccagatg catggctggc tacttatgca gatactataa cactgattct tacatttttc
    10861 gtattattgt atgcaacatc agttgtagat atggaaaaat ttaaaaacat agcagatgcg
    10921 ttaaaaggtc agttttctgg gacaagtata tttagtacgg gttcaagtac aagtgagaaa
    10981 tctccaatag acagtgtttt agtggataaa aatgataaat caaatttaga acaggattta
    11041 aaagataaag ttaataattc taacttacaa aatagtgtaa cagtcaaaga tgatagtaga
    11101 gggattttac tagagttaga tgattcaatt ttatttgact ctgggatttc tgagttaaaa
    11161 gtagatagta aagctactct taataaagta tatgacatga taaagatgat gaaaaataag
    11221 atagttatag aaggccatac tgataatgta cctgtaaaat caagtacaca tgaatccaat
    11281 tgggatttat cttctagtag agcagtaagt gttgtaagat actttgtaga agaaaagggc
    11341 atggacccta gactattttc ggcaacagga tgtggagagt atcaaccttt ggtttcaaat
    11401 gaaacagcaa agggaagggc tgagaataga agagttaata tacttataac tattgactag
    11461 gaggaagagg tttgttcaaa aataaaaaaa ttattataat tattttgata ctgattattt
    11521 tctcagcggg agcaggtttt tttatattag gagaaggtgg aaataaacaa gttgacaaga
    11581 ttgcagattt gtttattcca acaggaaaag gtaaagatga agtaaagacc gaaaatcttg
    11641 acttagaaga gatggtatta aagctagctg atgaaggtgt aagatattta aaagtatcaa
    11701 taactgtatc ttatgattct tcatataaag atatagaaaa aaatacgcct ttaattaggg
    11761 ataatattat aaattgtttt atggcaaaaa gggcagatga ttttactgct gataatttaa
    11821 agaacataaa agaagaaata aaaaatacat taaatataca actaggtgaa aatgtaattc
    11881 aaaatatata tttcacaaat atagtagtgc aataaaagga tggtaagaca tgatacttta
    11941 tttactaaaa atattaatat gtatcccaat aattttaata ttgatagtct tatctttaaa
    12001 aattagccaa ggaactttat taaaaaaata taatgataaa tatgtaaaaa tactagatat
    12061 tgtaaatata agtaaaaaca atagcataat aattttaaaa ataggtgaag aaggatgcat
    12121 tatggcatct tcttcttcta gcatgagtaa aataaaagat ttgaccaaag aagaaatatt
    12181 aaaaatagaa aacgacatta aagaaagaaa ttttaaaatt caagatttta aaatggaaag
    12241 aattaaaaag aaatatgatt tcagtagata tattggaaaa tttacaaaaa aggaagataa
    12301 gtaaatggaa ctattatcaa aattaccttt ggctgatgta ccatatacaa gttcaatgca
    12361 attgttttta tttctaactg cattaatgtt tttaccattt gtaatgttca tgatgacaag
    12421 ctttgtcaga atagttataa gtttatcatt tttgaaatca gcattaggag cacagcaagc
    12481 aattccaagt caaattttgg taggacttgc aatagtattg acaatattta taatgagacc
    12541 agtattaaat gagataaacg aaaaggcatt acaaccatat atgaaagaag aagttactat
    12601 ggaagaagct atgaaggaag cagaaggacc tattaaagag tttttattaa cacaaaccag
    12661 acagacagat ttagatttat ttgtagaaca ggctggttta aaagaaaaga aattaactcg
    12721 tgaaaatata ccactatctg ttgtagttcc agcatttgct ataagcgaac taaaaactgc
    12781 ttttcaaata ggttttttaa tatatatacc atttttgata atagaccttg ttgtggcaag
    12841 tgttttgatg tctatgggta tgtttatgtt gcctccagtc atgatatcat taccatttaa
    12901 gttattatta tttgtaatgg tggatggatg gaacttgata gtaaaaacat tgatattggg
    12961 atttggatag gagaaaaata gtatgagtga atttatgata atgagtgttc ttaaaaatgg
    13021 catgtttaca ggagctatga ttgcagctcc agtactcatt gtttctcttg tagttgggct
    13081 tttaataagt gtgtttcaag ctacaactca aatccaggag caaactttga cttttgtacc
    13141 aaaactaata gctattcttt tagtagtagc tattggtgga agctggatgt tacatactct
    13201 tattagtttt acttctgaga tattcaatat gatatctctt ataactaaat aagtaggata
    13261 catatgattt ttgtatttat tagaatagta gcattctttg gaacactaag aatactattt
    13321 ccagctggta cacctgcttt atttaagtca ctctttgcaa tcattatatc aatattgatt
    13381 tcaagtacta tgcaaattga atatgcaaca aatatagaca acattgtatt gtttacctta
    13441 tatggtgtaa atgagaccat aactgggatt atgcttggat atataaccaa cctatgtttt
    13501 tatagtatca gaatggcggg ttctatgatg gaccaacaga tgggattatc aatgattaat
    13561 atgtttgacc caaattcaat gactcagaca actttaattg ataatcttat gaactggaca
    13621 gcacttatga tattttttag catggatggt catcatgttt taattagagg tataaggtat
    13681 agttttgagt taattccaat aggaaaacca tttgtagata ataacataga ttatataata
    13741 aacatatttg tgcagtgctt tttaactgga ttcaaaatag caatacctat tgtactttgt
    13801 ctgcttatgg cagattttat tttagggctt atatcaagaa gtgtacctca attaaatgta
    13861 atgatagttg gtatgccatt aaaaatattg gttggtattg cattatttat catatcaata
    13921 cctttaattg tcaatcaaat aagtcattta ttaagtcaaa taccaaagat gtatgaggga
    13981 acttttgcac tagctcctat gttttttatg ggttcagcag ataaaacaga agaagctact
    14041 ccaaagaaaa agggagaaca aagaaagaaa ggtaatatag caaagagtag agaattaccc
    14101 gttgcaatga ctctacttgc attcacctta ttagtaccta cattattttc atatgtagta
    14161 gatacactta aatcttcgct taattatttt ttgagtctag atttttatat gaacataaat
    14221 tattcaaacc tagagaaatt agttatagca ggattaatgg atttttttaa gatattttta
    14281 ccaatagcta ttcccttttt agttttaggc ataatagcaa acctatttca ggtgggaata
    14341 ctgtttactg gtgaaacttt aaaaccaaat ttatcaaaat taaatccaat tagtggattc
    14401 aaaaacatgt tttctatgag aagtttgtca accttaataa aggatacagc gattatatct
    14461 attttggctt acataggata tacattcttc caagataatt atttagatat attaaagcta
    14521 ggtaacatat acctaccaac tttaatgtac acagttaaag acttagtata ttcaatattg
    14581 agtaagattt gtgtagctat gatagctata gcagttgcag attatgttta tcaaagatac
    14641 agtcataaaa agcaactgag aatgacaaaa caagaagtta aagatgaata taaaaactca
    14701 gaaggagacc cagaagtaaa agctaagata aaacaaaaac aaagacaaat ttcgtctcaa
    14761 aggacaatgc aagcagtacc aagtgctact gtaatagtaa ccaatccaac ccacttatct
    14821 atagcagtga gatatgaaaa agggaaagac caagctccag tagtggtggc aaaaggtgca
    14881 gattatctag cttttaaaat aagggaaatt gcaaagggaa ataacatccc tataatagaa
    14941 aacaaaccta ttgctaggtt gttatataag caagtagaaa tagaccaaga aattccagag
    15001 gacatgtatc aagcttttgc agagatatta gtggcagtat ataaaatcaa aaatagatac
    15061 aaagtcccca aaaggtagga taagtaatga ataagtttaa tttaaaggaa aatatggatg
    15121 taatagtggc atttggtgtt gtagggattg ttcttatgat gatagtgcca ttacctacat
    15181 ttatattaga tattttactg gcaattaaca taagtttatc tgtgctaatt ttactaatga
    15241 cattatttac aacaaatgta cttgatttat ctatatttcc aacagtgtta cttgtgacaa
    15301 cactatttag attgggatta aatctttcat caactagatt gattttaaca cagggatatg
    15361 caggtgaagt tatagaggca tttggaagtt ttgttgtagg tggaaattac gtagtaggaa
    15421 taattatatt cttaattata ttaattgttc aatttgttgt tataaccaat ggttctggaa
    15481 gagtatctga agtaacagct agatttacgt tagatgccat gccaggtaag cagatgagta
    15541 tagatgcaga tttaaatgca ggagttatag atgacaaaac tgctagaaaa agaagaaaag
    15601 agcttcaaca agaagcagat ttttatggtt ctatggatgg agcaaataag tttgtaaagg
    15661 gagatgccat agcaggtctt atagtaacag caataaacat aataggtgga attgtaatag
    15721 gagctgtaat gctgaatatg actcttatgg gtgcagcaca gacatatgta agacttacta
    15781 taggggatgg tcttgtaagt cagatacctg ctctattaat atctacatca gcaggtataa
    15841 tcgtaacaag agcttctact gatgaaaact ttggtgactt agtaggtaaa cagttaacgg
    15901 caataccaaa agcagtaatg atgactggtt gtgttctaat cgtacttggt atgatgcctg
    15961 gattacctaa accagcattt ttttcactag gtataggagc tatagcagca ggttactttt
    16021 taggaagaga aaagaaagat actttagaag acttggagtt tgaaaaacct gatatggtaa
    16081 acccagataa aaatgaagta gaagatgtaa caaatttaat aaatgtagaa tcaatagaag
    16141 tagaaattgg atatggttta atatccttag ctgatgaatc agcaggagga gatttacttc
    16201 agagaatagc atcaataaga agacaatgtg caatagatat gggtatagtc gtacaaccca
    16261 taagaataag agataactta cagttaaatc caaaccaata caatataaaa ataaaaggaa
    16321 atgtagttgg tgggtatgag ataatgccta atatggtttt atgtatggac cctatgaatc
    16381 aaggatttag tatatttgga ataaagacaa ttgaacctac ttttggaata gaggcacttt
    16441 ggatagaaag tgacaaggta gaagatgcag agttaaaggg ttatacagtt gtagattcat
    16501 ccacaatact tataactcac ttactagata taataaaatc aaatgcacac gaattactgg
    16561 gaagacaaga agttaagact attatagatg cagcaagaga aaattatagt gctgtagtag
    16621 atgaattgat accagatttg atgacacttg gtgaaattca aaaagtactt aaaaatctgt
    16681 taagagaaaa agtaaatata aaggatagag ttacaatact tgaaacgctt gcagaccagt
    16741 ctagaaatac aaaagatata gagcttctta ctgaatatgt aagaatagcc ttggctagaa
    16801 gtatttgtaa taatttagtt gatgatgata agacaattgt agtggctaca ttgtctccag
    16861 aaactgaaga gttggtatct aataatttac agaggtctgt aaatggaact tatccagcag
    16921 ttgacccaga aaatacaaat aaaatatttg aaagtataca agaagtaatg agcaatgtat
    16981 atttcaatca taatatacca gtaatagtag tatcaccaaa gataagagct ccatttagaa
    17041 aattagttga aattgtatat cctaatttga ctgtattatc attaaatgaa atacctaacg
    17101 atattaaaat caaagcagaa ggtgtggtga gcatatatga tgacgaaaaa gtatacagct
    17161 aacacaattc aggatgctat gaatctagcc aaaatggaat tgggtgacaa tattacatta
    17221 atagataaaa aagaggtaag aaagtctgga atacaaggta tcttttcaaa aaaagatatt
    17281 gaactaacta ttggatggga aaagagagaa aatgtagaac aaaaagattt aaaacgagag
    17341 atagaacaat taaagtcaat tattaacaat atggggtttg ataataagaa tgataatgat
    17401 atagataaaa tatgtaaaaa tctactaagt ttagaattga atgaagaaat tgtagaatct
    17461 ataagaattg atttgcaaga aatgaagttc aatggaatcg atacaagtaa aaatttagta
    17521 gaaattttaa agaaaaagat taaaatagaa aatcaagtaa taaatggtaa aattgctcta
    17581 gtcggaccac caggagtagg taaaacgact acaatagcaa agttagccgc aaaattagtt
    17641 tttgaagaaa ataaaaaagt aggagtaata actatagata catacaggat tggtgctgta
    17701 gaacagttga agatatatac tgatataatg aatattccct ttaaaggggt aataagtcct
    17761 gatgaaatgg aattggcact tgacgagatg aaagattgtg atgttgtatt aatagataca
    17821 acaggtagag gctacaaaaa ctctatgcaa atactagaaa tcaaaaatct tatagataaa
    17881 gctgaaactg ataatataca tttagttttg aattgtacaa caagagaaag tgatacaaaa
    17941 gcaattatag attcatatag aaatgtgaat tttaaaagct taataattac aaagctagat
    18001 gaaacaataa catatggctc aatatttaac ataatgaatt atgcgcaaaa accaatatca
    18061 tatattacaa caggacaaaa tgttcctgat gatattataa aacctaatga agataagatt
    18121 ataaggttac tattaggagt tgaaagtata tgatagacca agctcaaatt ttgagaaaaa
    18181 tagcaatgga aaaaagagga cttgatgagt ttatagtaga gaatgagaat gtacctaaga
    18241 taattacaat agcttctggt aaaggcggtg ttggaaagag caatctcgct acaaatttgt
    18301 ctatatgctt gaccaaactt aataaaaaag ttcttatatt agatgcagac ataggaatgt
    18361 ctaatataga tataataatg ggggtaaatg taaaagggac tataattgat gtaattaatg
    18421 gcgaaaaaaa tatagaagat ataatttcac agactaagta tggtgtaaat ataatatctg
    18481 gaggttctgc ccttaatcat atagaagact ttacagaagc tcaaagaaat aagtttatac
    18541 atagcataga acaaattcac gatgtggatt ttataataat tgatacagga gctggtatga
    18601 gcaaaagcct attgtcattt atatactgta gtactgaatt tttcttaata acaactccag
    18661 aaccgacatc attgactgac gcatatagtt tattaaaagc tataagtaat tttggcataa
    18721 agagaagtgc aaatataata atcaataggg taattgattt agaagaagca aaatctacat
    18781 acaaaagaat aaaaatggtt gtggacaaat ttctaaacct taatctagaa ttgtatggat
    18841 atataataga tgacaaaaaa gtaagtttat gtgtaaaaaa acagatacca ttcattttag
    18901 aatatcctac aagtattgca tcaaaatgta ttatagccat agcaaagaga atggtaagtc
    18961 agtcaagaga agatattgtt gataataaaa gttttctaag aaagatgttt agtatattta
    19021 aaagctaagg aggcgtagtt aatgaataga gaagaattaa taaaagagaa tatgcctctt
    19081 gtaaagagta tagcaagaaa attttttata ccagggaaag gttttgaata tcaagactta
    19141 gtaaatagtg gagtcattgg attgattgat gcaataaata aatttgatgc tgaaaaaggg
    19201 gcaaagtttt ctagttactc ttatataaaa ataaaatctg ctatattaga tgaaattaga
    19261 aatcaaagtc caatttctaa acataatcta actaaagtaa ataagtataa tagagtggta
    19321 gaaaagttgc aatctaaatt attaagagag ccaacttcat atgaaattgc aaaagaatta
    19381 aaggtttcag aaaaagaact tcatgatatc gaaagtaata tagatatgtt aaatattgta
    19441 tcattaaact atgtaatctt tgaagataca aatgaaactg tacaagatgt aataagtgat
    19501 agagaagagg aagctccaga aaatataata gaagaagaag aaaagttaga gattctatcc
    19561 aaagcaatta gtaatttaaa tgaaagagaa aaattaatac tttcactgta ctattatgaa
    19621 gatttaaatc taaaggagat agggaaagtg ctaggtgttt cagaatcaag agtttcacaa
    19681 ctccacagaa aatcaataag aaacttaaga aacaagataa aagaacttaa atattctata
    19741 tagatggtga ttgataagat gatacaaatt atattaatag tagtgtctat tttaataata
    19801 gtaactatag ttataaattt acataaggaa tcaaaaaata aagataacag atactttgat
    19861 aaaatatttg agcaagaata tgaagcaaat gatagattaa taattgcaga aaaaagaaga
    19921 ttctttttag acaaagtact tgcagaaaaa agaattaata gtcatataaa agtttatgaa
    19981 aattataacc taataaaggc taatgaagaa agaaaggaaa acatatttat agaatcaaaa
    20041 aaggaagtaa gacaagaaaa tgagttatct cttaaagtga aacatcttat aagttcaggt
    20101 tatgatgatg tggaaatatg taagatgcta gatattggaa agggggaact gttattaata
    20161 aggagttgtt acaaaatata aggatattat tatgtgataa aaaagtattc ttaggaatag
    20221 gtattggtat attaatatca actttatgta taatgctttt tagtaataat gatatgagta
    20281 aggcagagat agaaaaaaag gccagagcgt taggcatgaa atatacaagt gagatgaaag
    20341 ttttagaata gatgatattg tttagacatt tacaaaaaag gaggtcataa tgtgataaga
    20401 ggattatata cagcagtttc tgctatgata actaatcaac aaaagcaaaa tgttgttgtc
    20461 aataacctag ctaatatgga tacaaatgga tataaaagta aacaactttt gactaaaagt
    20521 tttgatgaat taaagttgga gggttacaat aactacgcaa atggaactaa acaaaagcag
    20581 attataggcg atataagtcc aggtatttct atggatgaaa ctatcacaaa ttttaatcaa
    20641 ggacctataa aaactactga taataaaatg gatgtagcta ttcaaggtaa gggatttttt
    20701 ctagttagtg atgcagctgg aaatcaattt tacactagaa atgggaactt cagaacagac
    20761 aatcaaggtg accttataac aagtgaaggg tattatgtac ttgggacgaa tactgctact
    20821 ggtgctgtag gtcatataaa tgtaggaaat caaaaatttg aagtggcaaa agataataca
    20881 atatcactag acggaaatgc tatgtataaa tttaatattg tagattttaa taactacaat
    20941 aacttaaaaa aagaaggaga taacttatat tctggaggag gagctatgaa agtaaataat
    21001 gcacttacaa tacaaagtgc gatagagtcc tctaatgtag atatggtatc tgaaattaat
    21061 aatatgatga caatatcaag agaatatgag gcaaatcaaa agataattca ggctatggat
    21121 tctaaattag caaaaatagc aagtgagatt ggttcagtaa gataggtaat aggaggtgaa
    21181 tttttatgta cacaatgttt tatactagta aaacagcttt atcagcaaat caaagtaaac
    21241 tagatataat atctaacaat atagcaaatt ctgattcaac aggatataaa aaaataaata
    21301 tggaatattc tgatttagtg gatgaagtac taaatagacc atcatatcct acaaatggca
    21361 atgatatatc gactggaaca ggagctaagg caacagctcc catgagaaat tatgaacaag
    21421 gtgcattggc tcctacagat agtaagagta atttggcaat agatggagaa ggattcttta
    21481 gagtgataag aaaagatggt acatatgcat atacaagaaa tggtggattt aatgtagatg
    21541 ctttaggtaa gattgtagat gataatggaa atatattaga tgtccaattt gatgcaggat
    21601 ataactataa taatacaaat atagatagta caaacctgac agtttcaaga aatggagagc
    21661 tattcgtaga taataaaaaa ataggaaaaa tcaacttata tcaacctata ggaactcaaa
    21721 attttatatc tgaaggagat agtttattcg tcgcaactga tgcagcacag attaaacaag
    21781 ttgagaaagt cgatatagtt caaggctata gagagaggtc aaatgtttct ttacaagaag
    21841 aatttgttga cttaatagcc actcaaagag catttcaaat gaactcaaag ggaataaaga
    21901 cagcagatga aatgtggcaa atagcgaata atttaagagc taaataatta tgtaaggtga
    21961 ggaatattca tgaatgatat gaaagagtac gattttggca aacctcagag ttattcaaga
    22021 gaaaatttaa cttgtttaaa ttttttatta gctgaatttt gtaaaaaata taaaaactat
    22081 ataatttatg aacttaagtg taattctagt atggttgttg atagtataga ccaagtaaat
    22141 tatcagactt ttttagatag agtaacttct tcatgcgtag tagtacaaaa tgcaatggaa
    22201 cctgttatga aaaacttttc ttttagaata gatagaagtg tagctgatat gtggattgat
    22261 ataacttctg gaggaactgg agttgtaaag gataatgata gagaacttac agaacttgat
    22321 aaaaaagtgc ttttacacct tatggatgat ttagtaagaa atatgcactt atttgagggt
    22381 tttgaaaata tacaagtatt ggatacacat acacatatta atttgccaca attatgttct
    22441 ccaactgcac cagtatgcgt tgtagattta aaagtgctaa atgataatga gtatattgga
    22501 agtgtttctc tttgctttcc atacaatggt ttagaggctc tgttagaaag tatatctgtt
    22561 atagaatttt ttgataatga tgtaggagat gattcagagg aatttaccca aaaaatctat
    22621 aatagtgtat ctaatataga attatcagtt attgcagaga taggaaaagt atcaattaat
    22681 gttgaggatt tactcaaact agaagttgga gatgtaatag taacaaataa aaaaataaat
    22741 gattatatag atatatttgt agaaaattca aaatcataca cagcaacacc aggatttata
    22801 tcaagtaaaa aaggtgtaaa aataaatgat gctgttggaa aagaggtgta gttatggaca
    22861 atagtaatct tatttcagat gaagaagcaa agatgttatt aaatgataaa gttgaaagtg
    22921 aagttgatgg agatgatgag ttagccttag atgtctctga agttaatcga aataatatac
    22981 aaaggatact tgaccttaag ttagaattat ctgtagttat aggaagaact aagatgactc
    23041 taagagatat tttaaattta cataaaggtt ctcttattga actggatact ttggcagacc
    23101 aagatgtgga aatactaata gataataagg ttttggctta tggaaaagta gtggtggtag
    23161 atttaaattt tggtgtaaag ataacaagta tagtaagtga agaagacatg gtaaaatctc
    23221 ttgtttagag ataacatcaa ttaatactta aggcatgagg tggtacttat tttaaactta
    23281 aatgatataa aaaaagatat actaaaactc aataaaaaag actcatgttc tctatataat
    23341 gaatctatta ataatataaa taataattgt atatcaactg gattaaaatt tttaaaacaa
    23401 gcaagagatt taaacccaga tgatgttgat atattaaatc tcacaggtct tgtaaactta
    23461 ctaaaatgta attttgatga tgctgtagaa agtttctata agagttcact ttgtgaaaaa
    23521 actgctctgt gtgaaaagta tataaacatg cttacatctg aggagttttt aattttcttt
    23581 gaaaagtaca atcaacttat aaggttaata aataaaggaa tgtataaaga agctatacaa
    23641 gggtttaaac ttataactga ccaatactgt gatttaatag aaccttatga gttgcttgcc
    23701 ttactgtatg acaaggaaga agaatatata aaatttgatg aatgtttaga agtattgaag
    23761 attattgata aggaaaatta tttattaaat caagaaaata acacattata tatgtataat
    23821 agtgctaata tacaaactaa aagtaatata aaagtagatg attataagaa tgagttctta
    23881 cataaaagta gtagtaacca taaaattaat gattataatg atgtaactaa agaaaagagt
    23941 tcaagcaaaa accagaaaag ttcagattct aataagaaaa aaagtattaa aaaaaataag
    24001 tacctatata tagccataat attaggaata ttattagtag gacaaagtat atatagttct
    24061 tataagatga ataatttaac tgataaaata tctaaaaata atgaaactaa aaatgaagag
    24121 tcaatagatt ctaacttgac taagaaagaa aataatatag aagaaaatga agatatgagt
    24181 aaggcttcta atataaataa gagtaataaa aacattaata aagaggatga tgtaaaatct
    24241 aaaaaaaata ctgtagaaaa tgaagatgat aaagattctg acttatttac agaggatgaa
    24301 ttgatgtcta aggcgaatac tttaaaatca gaaaagaaaa ctaaatcttc tatacaacta
    24361 tataagaaag ttgcagatat aggtgaaaat aaagggaata catctgaagc aacttatcaa
    24421 gttgctattc tcagtgaaaa gttaaaagac tataaaactg ctgaagaata ttataaaatg
    24481 tatgtagaaa attactcaga aaaagatgct tattttgatg aatcttatta taatttaggt
    24541 atgatgtact ataacaatgg tgatttaaaa aattctaaat tgacattaaa gaagttggtt
    24601 aataaggtac caaatagtat gtataataat tcaaaagtaa aagaaatttt aaaagaagaa
    24661 tagttaaatt agataagaaa aataaattat tttattttaa aataatatga ttataatata
    24721 ttgacatacc attttaaaaa atttaaaata ttaattataa aatgaggagg gataaaaaat
    24781 ggaatttgaa aaagggagta tttcaattca tacagaaaat atttttccta taattaaaaa
    24841 atggttatac tctgataaag acatatttat aagagagctt ataagtaatg gatgtgatgc
    24901 agtaagtaaa cataaaagac ttgtatcact aggagaaata tcagaaaata agtcatcaga
    24961 ttataaaatt acagtatctg taaataaagg agaaggtaca cttaaattta ttgacaatgg
    25021 aattggtatg actgaagaag agataaaaaa atacataaat caagttgctt tttcaggtgc
    25081 tgaagatttc tttaataaat ataaggataa aatggaagag tcaaatgata taataggtca
    25141 ttttggatta ggattttatt ctgcatttat ggtgtctaaa aaggtacaaa tagatacact
    25201 ttcatataca gaaggagcga ctcctgtaag atggatatct gaaggtggta cagaatacga
    25261 aatatctgaa tcagatgcta gaaatgatag aggaactact ataacactat ttattgatga
    25321 tgatagtaaa gaatttttag atgaatttac tgtaagagga ataataaata aatactgttc
    25381 tttcttacca gtagaaatat atttagaaga tgttgaaaga ttagaaagag aagctaaaga
    25441 agcagaagaa aaagctaaaa aacaaaagga agatggaaaa gaagagattg tagatgcaaa
    25501 agtaattgag cctttaaacg acactaatcc tctatggtta aaatctccaa aagactgtac
    25561 tgatgaggag tataaagagt tttatagaaa ggtattcaat gtatttgatg aacctttatt
    25621 ttggatacat ttaaatgtag attatccttt caatttaaag ggtatacttt acttccctaa
    25681 attaaaaaat gagtttgaac tgactgaagg aaaagtaaaa ttatacaaca atcaagtgtt
    25741 tgtggctgac aatataaaag aagttatacc agaattttta cttttattaa aaggagttat
    25801 agactgccca gacttaccat taaatgtatc aagaagtttc ttacaaaatg acagggatgt
    25861 aagcaaaatt tctaaacata taattaaaaa agtagctgat aagttgaaat ctctttgtaa
    25921 aaatgaaaga gaagaataca ataagttttg ggatgatata caaatattta ttaaatatgg
    25981 ttgcttaaaa gatgaaagtt tctatgagaa ggtaaaagaa tgtatattat tcaaaactat
    26041 tgatgatgag tatataacat tacaagatta tttagaaaaa tgtaaggaca aacatgaaaa
    26101 taaggtattt tatgtaagtg ataaggaaca gcaatctcaa tacattaaac tatttaaaga
    26161 atacgactta agtgctgttg ttttaaactc atcaatagat actcatttta tttcatttat
    26221 ggaatataaa gagaatggag ttaagtttaa tagaatagat gcagaccttt ctgatgtatt
    26281 aaaagataaa aatgaaaata aagatagtga agaaaacaaa gaagagatag ctaagataga
    26341 aggtctgttt aaagaagctg tcggagaaag agtgaagaat tattctgtag aaggattgaa
    26401 aaatgaagac actccagcta tggtattagt atcagaacaa tctattagaa tggcagaaat
    26461 gcagtcaaga tttgctggaa tggatttagg aatgaatttt gaggaagaaa aaactcttgt
    26521 aataaatgag aacagtccaa taatcaaaaa attggtttct cttaaagatg atgaggaaaa
    26581 gaaagataag attacgctta tttgtaacca gatagcagat ttagctttac tttctaataa
    26641 agaattaaag ccagatgagt tagattcatt tgtacaaaga agtaataagc ttatgagtct
    26701 tttgatagaa ttataagatt gaatttgaat acattgaagc aaatttataa aaagtattca
    26761 atatcagtga taagaaagaa gtgtctcaaa atgaactttt cagttcatga ggcgcttttt
    26821 ttatgtgaaa ttaaatttat tttaatcatt tcaacaatga aaaatatatt tatctctatt
    26881 ttgagacaga ccattcttta tttttttatc tttggttaat tttaaatatt taagtaggtt
    26941 ctaataaaaa actctattta gttaaatata gttaacttga tatataataa gataatacat
    27001 ataattaagt aaaagattgt tttttagttt atgtttaaat acataattac actttgttaa
    27061 aaacataaaa ttattactaa agtagaaata ggaggtttta gaatgaattt taattataat
    27121 ttgccagtaa atatcttatt tgggagaggc agaattaatg atgtgggaaa agaagtatct
    27181 aaatatggca aaaaagtatt gatagtaaca ggtaaaaata gtacaaagaa aactggatta
    27241 cttgataaaa caatagattt actaaaagac tctaaaatag aatatgaagt ttttgataga
    27301 gtagaacaaa atcctcttat aactactata tattcaggag tagaaattat aaaatcaact
    27361 ggatgtgatt gtgttttagg tcttggtggt ggaagcatta tggatgctgc caagagtata
    27421 gctttttctt ataaaaaccc aggagattta aatgattata tatttggaat tagacaaggt
    27481 gaagaagctt tacctataat cttagttcca acaacatcag gtacaggtag tgaaggaaat
    27541 tgttttgcag tacttacaaa ttcagaaact aaagataaaa aatctataaa gaagaatagt
    27601 atgtatgcaa aagcatcaat aattgaccca gaacttatgg ttacaatgcc aaaacatata
    27661 attgcatcag ttggatttga tgcacttgcc cataatatgg aagcatatct ttctaatgga
    27721 agaaaccctc ttgcagatgt acaagctatt tatgggattg aacttatatc agaaaaccta
    27781 ataaaggtat ataatgatgt taatgattta gaagcatggg aaaaaataac acttgcaagt
    27841 actattggag gaatgacgat tggtacagcg ggtacagcct tgccacatgg aatggagcat
    27901 cctgtaagtg gtcttagaga tgtaatgcat ggaaaaggac ttgcagcttt gactcctact
    27961 attatagaaa gttcatggga tagtgatatt gaaagatttg gtaatatttc aaaggtatta
    28021 ggaggaactt gtgcagaaga ttgttcagat gcaattagaa atttccttaa aaaaatagat
    28081 ttagatataa aactaagtga tttaggtatt gaaaaatcag atatagattg gttaactgat
    28141 aattgtatga aaatatcagt tgcaaatatt aagagacatc caaagtattt taataaggaa
    28201 caaataaagg aaatgtacca taagtcttta taatttgact ttacatattt agttttatat
    28261 aaaaattata aattaataat aaaaaattat aagtaattaa tagtaaaaaa attgtgtata
    28321 gaagttatat atataataaa tttgtcttaa gcttttattt tactttctaa taaataaagg
    28381 agtttaaaaa agggaagatt aattaagtaa tattttataa aattcttcga tttaaaaact
    28441 ttaagtagag gtgaaataaa tgtttaatcc caaaagaata atattcgaaa aaggaacttt
    28501 agacactgat gtgggtaaaa aaatatacaa taaatttaga gataataaag atgtagagat
    28561 aattaatata tcatcaaata ggattaaaga acatattcca ggtgaaaatc tatatgaaca
    28621 atatagaagt gggaagcaga ctatggtagt tgggattaga aaaagtctga agtttcaaac
    28681 atgtaaacct tctgctaatt atcaactacc acttgtcagt ggatgtatgg gaagatgtga
    28741 gtattgttat ttgaacacac aattaggtga taaaccattt ataagggtgt ttgtaaatgt
    28801 agatgaaata cttgaacaga caaaaaaata tattgaagat agaaagccac aggtaaccat
    28861 atttgaaggg gcagctacat cagacccagt tccagtagag ccatatacta attctttaaa
    28921 gaaaacaata gagttttttg gaagagagga aaaaggaaga tttagatttg ttactaaata
    28981 cagtgatgtg gatagcttat tggatgctga acataataat catacagaaa tacgatttag
    29041 tatcaatact cctagaatta taaatgaaca tgagcattat acagcctctg caaataaaag
    29101 aatagagtca gctgtaaaac ttgctagggc tggttatcag attggtttca taatagcacc
    29161 tgtgttttta tatgaaggtt ggaaagaaga gtataaaaat ctgattataa gtataaaaga
    29221 agctttacca aaggattttg ataaaaaaat tatatttgaa gttatatctc atagatatac
    29281 aactaaagca aaaaatagaa tactagagat ttatccagat acactattgc caatggaaaa
    29341 tgaggataga aagtttaaat acggtcaatt tggatatggt aagtacgtgt atgataaaga
    29401 acaattagat gatatgaaag agttctttct taaggaaata aatggagtat ttaaaaatag
    29461 tttaataaaa tatataatat agcaaatctt acctaaagat ttgctatatt ttatttttat
    29521 acattttatg tttataataa agcaatgaga tttagcacta tcatataaga aaattaaaaa
    29581 gggcactatc tatcaagaac attttttcta gttttgatac tataaaggat aagagtaaca
    29641 aaaatatcag gaggagttat agtatgcatg catgggagtc tattcaaatt acattggatt
    29701 taatagaagc aaatttatca gaagaaatat caattaatga attagcaaat aaagcaaatt
    29761 tatcaccatt ttactatcaa agactattta aaagattggt aaataagact gtaatggagt
    29821 atataaagct tcgtcgtctt gcaagagctt ctgaatactt agcagaacat aagaatcgta
    29881 tacttgatgt agctttaaat tttggatttg ctagtcatga gacatttaca cgttccttta
    29941 aaaaagcata tggattaact ccagaaaaat atagagcaaa tccagtaaaa ctcaatcact
    30001 ttattaagcc agaattaatt ttaaattatg ctatggttga tgaaggtgtt ccactgattg
    30061 cagatgatat agtgattgaa gtcaatcgta agacattgag taatccaagg acatttgttg
    30121 gaatagaaat agaagtacct atttgccagt tggtgggcgg tgaaacaact ggaattgcaa
    30181 tagtagagga attatggatg aaacttggaa gtcaaagaca taatatacca aatcaaattc
    30241 caggtggaaa tgaatttgct gctctttata tgggaaatgc aaaagaagga aattgtatgt
    30301 atatggcagg tgtagaggta gagtcaggta catcagtaga aggatatagt acttttgagt
    30361 tgccagctaa agaatattta gtatgtggat ttgaggcaga aaactttaat gagttagtta
    30421 attcagctgt ttttaaagca gataaattta tggaaagatg gatgaagaaa cataatttaa
    30481 caactactga ttttgcaatt gagatgtatt accccactac acctgaagca gcatatctgg
    30541 aacattggat tgttccggtt cctattgaac aatagaaagg atgatgaata tgaattggca
    30601 agatgcgttt tttcaagata ctaaacctac atttgagcaa attaatgaat ttattaataa
    30661 tccattatgg gacaagttaa ataaaacttt ggtttctact tataatgtag aacctaagct
    30721 tgaatacagt aaatgttcaa tgcaaagagg ttggaatgta aaatataaaa aaagaggtaa
    30781 atcactatgt actttatatc cacaagaagg ttattttaaa gctttggtta ttgtaagtga
    30841 aagcaataga gtagaggttg atttatttat taatacttgt tgtgattata tcaaaaaaat
    30901 atataatgag gtaaactttt ttaatggaag taaatggcta atgattcaag tagatagttt
    30961 attggtatta aacgatatgc ttgaactaat taaatttaga gcttaacata tagaacagga
    31021 tgaatatata agtcatcctg tttttatatt caatcagata aattaatata tagtctataa
    31081 tatgaataat ttagtaaatt taaatttact ttatctttct acattctttg caactaagtc
    31141 cacatgtgtt tttttcttgc atattagtag caccccaatt agcaaattgt tgtaaaatag
    31201 gcacaatact tttaccttta cttgttaatg tatattcaac ttttggagga atttcattat
    31261 attgtttacg atgaacaata ccagtagact ctaaatcttt taatgattga gctagcatag
    31321 tatttgtaat gccatccaat tttcttttta tgctactata tctaattgga ttttcaactg
    31381 ctaacataca tataataggt aatttccact taccaccgac aatgtctaat gcataacgga
    31441 ttggacaggt taattcttca acgtttttag ttatattttt ttcagtattt atactcattt
    31501 taattactcc ttagttatag tagtatcttt tttatacttt atcagaaaaa actgcatact
    31561 tgttttttct gtattttata ttaaaattat atcaaaggat aatccttaat acaagtattt
    31621 aaaaggggtg taagaattat gaaagcatta gaatttttga acaaggcacg agtttattat
    31681 ttagcaacag ttgatgagaa caatcaagca catgtgcgtc ctttaggatt tgttatggaa
    31741 tacaatggta aacttacatt ttgcacatct aatcaaaagg atatgtttaa gcaacttatt
    31801 tttaatccaa aggttgaact ttgttgtata gaccaaaacc ttaatactct acgtatttta
    31861 ggtgaagcag tatttgttac atcaaaagaa acacaagcta aggcacttga aataatgcca
    31921 agtttaggtc agatgtactc tgtaggagat ggtaagtttg aaatattctc tattgataag
    31981 gcaaaagtta gttgttgttc aatgagtggt aaaaagattg atgtagagct ttaattataa
    32041 ttgaatcgac atccatagtg aaaattaaga aaacgttatt actgaaatgt tgatagattt
    32101 aatttaaatg caataaaaag atttttaaat aatgaatatg taaaaatttt tagttattag
    32161 atgtgtattt caataatgat atactatttt gaataattac atactagaaa taagataa
//
LOCUS       DAMKMI010000032        30690 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMKMI010000032 DAMKMI010000000
VERSION     DAMKMI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.033"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            165..2066
                     /locus_tag="PC429_001396"
     CDS             165..2066
                     /locus_tag="PC429_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3846911.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            2069..3112
                     /gene="ypdE"
                     /locus_tag="PC429_001397"
     CDS             2069..3112
                     /gene="ypdE"
                     /locus_tag="PC429_001397"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF3846912.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            3127..3432
                     /locus_tag="PC429_001398"
     CDS             3127..3432
                     /locus_tag="PC429_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3846913.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            3529..3864
                     /locus_tag="PC429_001399"
     CDS             3529..3864
                     /locus_tag="PC429_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846914.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            3896..5218
                     /locus_tag="PC429_001400"
     CDS             3896..5218
                     /locus_tag="PC429_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3846915.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            5228..6298
                     /locus_tag="PC429_001401"
     CDS             5228..6298
                     /locus_tag="PC429_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3846916.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            6303..7490
                     /locus_tag="PC429_001402"
     CDS             6303..7490
                     /locus_tag="PC429_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3846917.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(7687..8559)
                     /locus_tag="PC429_001403"
     CDS             complement(7687..8559)
                     /locus_tag="PC429_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF3846918.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(8953..9144)
                     /locus_tag="PC429_001404"
     CDS             complement(8953..9144)
                     /locus_tag="PC429_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846919.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            9364..10410
                     /gene="cobT"
                     /locus_tag="PC429_001405"
     CDS             9364..10410
                     /gene="cobT"
                     /locus_tag="PC429_001405"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF3846920.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            10442..11002
                     /gene="cobU"
                     /locus_tag="PC429_001406"
     CDS             10442..11002
                     /gene="cobU"
                     /locus_tag="PC429_001406"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF3846921.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            11012..11785
                     /gene="cobS"
                     /locus_tag="PC429_001407"
     CDS             11012..11785
                     /gene="cobS"
                     /locus_tag="PC429_001407"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF3846922.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            11805..12419
                     /locus_tag="PC429_001408"
     CDS             11805..12419
                     /locus_tag="PC429_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF3846923.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      12486..12665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            12795..14294
                     /locus_tag="PC429_001409"
     CDS             12795..14294
                     /locus_tag="PC429_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF3846924.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            14333..15709
                     /locus_tag="PC429_001410"
     CDS             14333..15709
                     /locus_tag="PC429_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF3846925.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            15710..16681
                     /locus_tag="PC429_001411"
     CDS             15710..16681
                     /locus_tag="PC429_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF3846926.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            16713..17783
                     /locus_tag="PC429_001412"
     CDS             16713..17783
                     /locus_tag="PC429_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3846927.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            17785..18672
                     /locus_tag="PC429_001413"
     CDS             17785..18672
                     /locus_tag="PC429_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF3846928.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            18660..19292
                     /locus_tag="PC429_001414"
     CDS             18660..19292
                     /locus_tag="PC429_001414"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF3846929.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            19301..20527
                     /locus_tag="PC429_001415"
     CDS             19301..20527
                     /locus_tag="PC429_001415"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF3846930.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            20524..21132
                     /locus_tag="PC429_001416"
     CDS             20524..21132
                     /locus_tag="PC429_001416"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF3846931.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            21122..21697
                     /locus_tag="PC429_001417"
     CDS             21122..21697
                     /locus_tag="PC429_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF3846932.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            21690..22442
                     /locus_tag="PC429_001418"
     CDS             21690..22442
                     /locus_tag="PC429_001418"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF3846933.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            22426..23547
                     /gene="cbiG"
                     /locus_tag="PC429_001419"
     CDS             22426..23547
                     /gene="cbiG"
                     /locus_tag="PC429_001419"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF3846934.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYHVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            23544..24269
                     /gene="cobJ"
                     /locus_tag="PC429_001420"
     CDS             23544..24269
                     /gene="cobJ"
                     /locus_tag="PC429_001420"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF3846935.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            24266..25018
                     /gene="cobK"
                     /locus_tag="PC429_001421"
     CDS             24266..25018
                     /gene="cobK"
                     /locus_tag="PC429_001421"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF3846936.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            25037..26515
                     /locus_tag="PC429_001422"
     CDS             25037..26515
                     /locus_tag="PC429_001422"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF3846937.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            26545..27450
                     /gene="hemC"
                     /locus_tag="PC429_001423"
     CDS             26545..27450
                     /gene="hemC"
                     /locus_tag="PC429_001423"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF3846938.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSAIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            27440..28939
                     /gene="cobA"
                     /locus_tag="PC429_001424"
     CDS             27440..28939
                     /gene="cobA"
                     /locus_tag="PC429_001424"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF3846939.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            28956..29921
                     /gene="hemB"
                     /locus_tag="PC429_001425"
     CDS             28956..29921
                     /gene="hemB"
                     /locus_tag="PC429_001425"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF3846940.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            30074..30652
                     /locus_tag="PC429_001426"
     CDS             30074..30652
                     /locus_tag="PC429_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF3846941.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        12436 a         3059 c         5665 g         9530 t
ORIGIN      
        1 attgaactat atattttatt attataaaat atttatatta aattgtggaa ttaaaatata
       61 ctaggatata aaattcttaa ataaaaagaa aagattgttt ttataaaaaa tattttttat
      121 accacttaaa aaaacacaat aataacactg agggaggtgg ggatatgaga gaacgcatta
      181 ttcttgaaac attaaaaaat agtagtggtt atctaaacat agattatttt gcagataagt
      241 tgggggtatc tactcgaact atcagaaatg aaataaaaaa gatagaaact ataaaagaat
      301 gtaatggttt taaattagaa tataagtcta aattaggata tattctaaat attaaagacc
      361 aagataaatt cgagcattat ctaagaaact taccctcaga tttagttgaa aatccagaac
      421 aacggttaga gtctataata gtagaacttt tagtaaatga aggttataaa actatagaac
      481 agttaagtaa aaaatttcta gtaagttcat cccaaataaa aaatgattta aaaaagatag
      541 atgaaaagat aaaggataca gaattaaaac tagagcgaaa ggctcattat ggaattaaaa
      601 tagaaggttc tataaaagaa attcaaagta tattggtgga tagttatttt agagggaata
      661 gaaatataac agagtacagg aataagttta tagataattt aaaattagct aatattagaa
      721 gtacaataaa aaatgtactt aatgaacatg atttagaagc aaatttaaca gaacttgagg
      781 aaatattagc acaaattatt atattgtata taagagttag tatgagagta gcgggaaatg
      841 ctagcgattt aaaattatat acagaagatt taattataga taaattatta gataaaattt
      901 ttagagataa aaaatataac cttaattatg atgaaaagta ttatttaaag gagcttataa
      961 aactaaagac taaagataag aaagccacta taaagaatat tgataagaat aaacttcaag
     1021 atattatgtt cgaatttttt agagagatag ataaaaaata taactctaat tttttagaag
     1081 ataaagagtt ctttaactta ttttacttac atattgcatg tttaatagaa cgtataaaga
     1141 agaaccacaa gataataaat cctttttcag ttaaaataag tcaacagtat ccaactgttt
     1201 ttaatttatc tattcaattt tcaaaaatca tagaaaatga gtatcatata aaaataagtc
     1261 aagatgaaat agggtttata gctactcaca ttgctgttcc ttttgaaaag agagaagaag
     1321 ctaattttaa taaaaaatat aaaatagcaa taatatgctc ttctggtgga ggtagtgcat
     1381 ttttgataaa tttaagactt aaagaaattt ttccaaatgc tgagataaga aatttttcat
     1441 tactagaaga aaaagctgta atagaatttg caccagattt gattttttca ataacaaatc
     1501 ttttatttga aataaatgct ccagttatac ttataaatga aattttagac gaattggatt
     1561 atttagatat taaagaaagt gttagatttg cagaccatat aggaaatata tcaagcccaa
     1621 aacaatatat attaggtcta tttgataaga atcactttag gtgtataaaa gataaagcta
     1681 agtataagga tatccttgat agtatgtcaa aatgtatagt tgacgagggc gcttgttctc
     1741 caacctatcc caaagatgta tgggaaagag aaagctattt gagtactatt tatacaaatg
     1801 gggtggctat acctcatcca atcgagatga caggaaacaa aaatataata tcagtagctt
     1861 tgatacaaac tgatattatt tatgaaaata gggtgcctaa aattattttc atgatttcat
     1921 taataaaggg aaatttagaa cttcataagc aaatatctaa atatttaagt aagattatgg
     1981 caaataagga tatggtagac atgctaaata agtcacaatc ttatgaagag tttatgtata
     2041 agttaaaaat ctatctggga ggttagacat gaatatagag ttttttaaga ggttgtcaga
     2101 agcagatggt atagcatcta atgaagaaga ggttagacat gttttattgg aagaactaaa
     2161 agcttatagt gataaaatta tctgtgatgg gctagggagt atcatctttt ctaaaataaa
     2221 agatgagagt gcaccaaacg taatgatttg tgctcatatg gatgaagttg gatttatggt
     2281 gagaagtata gacaaacttg gtatgattca cttaataaca ataggaggag taaaacctct
     2341 tgcacagttt gtacaaaaag ttagaattac aacaaaagaa gggaagaaaa tccctgctgt
     2401 cataaatgct acatataata atggtaaggc tgaaaatata tatgcagata ttggagcata
     2461 tacagaagaa gatgtatata acctagggat taatgttggg gatatggtaa catatacaac
     2521 aagctttgag gaatttactt taccagaccg attagtggga aaggcttttg atgataggat
     2581 aggatgtttt gtgatgggag aagttttaaa agaacttagg aaagaaaatc taaattgtaa
     2641 tattcacttt gcggctacaa gtagtgaaga ggttggaata aggggagcaa agacatcaac
     2701 acagttaata aatccagata tagtatttgt aatagatgta gcatgtgcta aaaatgagtt
     2761 tgtgagagac tatacaaatc agaaacaaat tgataaaggt gtaatgctaa tgcatagaga
     2821 taggactcta gtccctagta gaaaaatgat agattatgta atggaaattg ctgataaaaa
     2881 taatatacca cttcaacatg atatgtttga atcaggtggg actgatggag gagaagcaca
     2941 tctcgtaaat gaagggaaac cttgtgtagt aacttgtgta ccagttagat atggtcattg
     3001 tgcattttca atagtaagta ataaagattt agagaacatt ataagattgt atacacagtt
     3061 aattttaaat tttgatgaag aacaatataa acatataaga aattttatct aaaggagaat
     3121 aagaaaatgt caacagaagt acaaataatg gaacttatat caactgcagg agagagtaaa
     3181 tcgaaagctt ttgaggcttt aaaaaaagtg aagtcaaaag attttaaagg agcaaaattg
     3241 ttaatagagg aggctcgtaa aatagatatt gaagctcaca ggatacaaac agaattaata
     3301 actcaagatt taaatgaaga tgttgaaagt ccagcaatga gtcttttaat ggtacatgct
     3361 caagaccact atatgacttc tcagctagca aaagacttga tagaggcttt gatatattgt
     3421 cttgataagt aattgtaata tagaaataga ttatttatgt aaaaattctt ttaaaagttt
     3481 agatgctttt aagagataat aaaaatacaa actttaggag gaattattat gaaaatatta
     3541 ttatgttgtg caggtggatt ttctactaat atgttaatgc aaaatatgaa aaaggtggta
     3601 aaagagagtg ctaaactcaa tgaaaaagat tttgagttta cagctatacc agcagattca
     3661 atagaaagtg aagttgatga ttgggatata gtacttatag gtcctcaaat agctcacaaa
     3721 atagacttct taaaaggaat attagagcca aaaaataagc cttttgcagt aatagataaa
     3781 gatgtatatg gaaaaatgga tggagctaca gttatgaagt tggcattaat aacttataag
     3841 aaacatcaat tagctcaagg ataataaaaa taatgaggga aaagggagat tagatatgtt
     3901 ttcaaaattt gaagcattta tgaatagagt atttatgcct ttagctcata aggttgacaa
     3961 tcaacgtcac cttggttcta taaaggctgg tatggttgct atgacaccat tcacaatatt
     4021 aggaagtata tttgcaatac ttccagcact tccaaatatg cttggagaaa ataatccaat
     4081 aagcaaattc atacttaaca attcagcaat attggattta ccagtaagtc tttccatagg
     4141 tttaatagga ttatatgctt gtatgtgtat tgcttttaat ctggggaact actataaatt
     4201 atatatacca ggttgtgttg cactatcaac tttttctttt ttattcctag tagcatcatt
     4261 tacaccagat ggagctttag aaataggaaa tttaggagct aaagggctat ttacaggtat
     4321 gatagcaggg cttggttctg tggagttata taatttttgt aataagaaga atatcacaat
     4381 aaaaatgcca gaaaatgtac ctgattttgt atctaggtct tttgaattaa tacctactac
     4441 tataatagtt tgtggaactt ttatagttgc tcgttttatt tcattaaaag tatttggaga
     4501 attacctcca cagatactta ctcaattttt atctccatta gtaggaagta tggataaccc
     4561 atggtcagtt cttgcactta actttgctat atgtactata ttcttttttg gaatacattc
     4621 atcagtattt agtcctataa ctagacctat aatgattaca tttatagctg aaaacatagc
     4681 agctatgcaa gctggagaaa caatacctca tttctttaca gcaggagctt caagtgcatt
     4741 ctttggattt acaggatgtg gaattagtat aggttgtgta attgcatgta tgttatcaaa
     4801 aaatgcttcc tataagaaga ttggacgtgt atcattattc cctgcattat ttgggataaa
     4861 tgaaccaata ttatttggag caccgataat attaaatcca ataatgttta taccacatgt
     4921 atttggtgga gctataatag gtacactacc aatgtttttt atgcactggg gatggttagc
     4981 aaagccagta tttgaccctc catatgtagg ggtattctta gaaggatttt taactaatgg
     5041 agattggcat tctatattag caaatgcatt gcaattagta ttagcagttt tattatattg
     5101 gccattcttt aaaataatgg aacgtcaaca aaataataaa aaacaaaaag aagaaactaa
     5161 gaatatattc tctaaagaag aagaagactt attggatgat ttaggattgg atttctaata
     5221 ttttgttatg aataatacaa tggagagatt agtatcctta atctataaaa gaggtgttga
     5281 cgctcttttg attaaagcaa aaaatactaa aagatatata ggagctttat caggtagtgg
     5341 tgtatatgtg cttgttacta aggataaaaa ttatcaaatt ctagatggca gatatattga
     5401 tgaagcagat aaaaaaacga ctggtttcat aaaaaggatt gtatctcaag gttcttatat
     5461 accaactata attgagttat taaaagaatt aaatataaaa aagttagcaa tagaaaacca
     5521 agctatgagt atacaagagt atctttcact aaaaaatgaa ggttttgaaa tcaatttaat
     5581 aacaaatgaa ttatggaagc taagggctat aaaatctaaa gaagaaattg aacttattaa
     5641 aaaggcttgt gaaattacag atgaagtatt tgatgaggtt atttctgaaa ttaaaaaaga
     5701 tatgactgag ctagaagtat cagctctaat tcagtatcat gcattaaaaa aaggtgcaag
     5761 tggaatgtct tttgaaacaa tagtggtatc aggagaaaga ggtgcaatgc cacatggaag
     5821 acctacaaac aaaaaattga aagtgaatga agctataaca atagattttg gtgttgttta
     5881 tcaagggtat caatcagaca tgactcgtac aatatctata ggtaaacctc ctaaaattat
     5941 taaagagata tatgatgtag tattggaagc acaattatct gcaatagagt caataaagga
     6001 aggaactagg gcaagtgatg ttgacaaagt ggctagaaaa attatagata aacatggatt
     6061 tggcgaatat tttaatcatg gactgggtca tggtataggt cttggagatg gtgaagtccc
     6121 cactttaaat ccaaatagtg aagatatatt agtggaagga atggtaatgt cttgtgagcc
     6181 aggtatttat attccaaatg tagggggagt tagaatagaa gatgacattg ttataataga
     6241 tggaaaaggt gttccactca ataagacatc caaagagttt ataatattag gagagtaaaa
     6301 ttatgaaata taattttaat aaagtattaa atcgtaaaga aggaaattgt agaaaatgga
     6361 gcaaccatgt gattaaagaa aaattcggct taaatgaaga tgcaattcct atggatttag
     6421 ctgatatcga ttttgagtgt gctcctgcta taaaagaatc tatcttaaaa agagcatcag
     6481 taggagatta tagttatact tttataggag atgacttcta tgatgcgatt ataaattgga
     6541 ataaaagaag atttgatgta tatatagaaa aggattggat aaaattaact tttggtactg
     6601 tttcaaccct tcattatata gtccaagcat atactaaaga aggagagggt gtattgataa
     6661 atacacctgc ttatgaccct tttgcagagg ctgtagtaaa taataataga aagttgtgtt
     6721 gtagctcatt aaaattagat aacaatagat attatttaga tttagaggat atggaaaatc
     6781 aaatgaaata cgagaatata agattgctta tattctgtag tccacaaaat ccatcaggtc
     6841 gtatatggac aaaagaagaa ctatatcaag tgtcagagtt atgtttaaag tataatgtaa
     6901 ttttggtaag tgatgaaatt catagagata taatatttaa agattataga tttgtatctt
     6961 tatggaatgc tcatccagaa atttataaaa atagtataat ctgtgtatct cctaataaag
     7021 gcttcaactt aggtggatta aaaacatcat atgtattaat aagagatgta aaaattaggg
     7081 agactttatt agaaagacta aaaagtaatt caataacttc tccaaatgta tttgcaatac
     7141 cagcaatagt tgcagcatat aatgaaagcg aagaatggct agatgctatg actagttatg
     7201 tagaggagaa ttttgaaata gtttatgatt tctttgaaac taatatacca aaagctaaag
     7261 tgatgaagtc agactcttct tttttagcct ggatagatgt aagagaagta tttagaaatg
     7321 aagaagaatc taaagaattt tttagacatg caaacttaac tatggtagtt ggaagttatt
     7381 ttgtgaaaga tggagatgga tttattcgta ttaatgtggg gtgtcctaga gaaacattaa
     7441 atgaggctct aaatagaatc aaaaaaacgt atataagtat gtattgttaa atcataaaaa
     7501 aagtatctat ggtttatata aaaacacatt atttagttat caaatattac tggaattttg
     7561 tattccagta atattttttg attaaggaat attattgagg aatattaaat aatattattt
     7621 cataccctta tattaactta tataaaaata ttttattatt ttgggaacgg gttagctaac
     7681 ctttatctag tgtatattta tattgtaaga agcagtaaaa acttttcctt tactaagttt
     7741 attaataaac ttcttatctt tataaatttt gttagaattt atactatcag caagaccata
     7801 ccaaggttct atacataaaa aaggagcagt agaattagtt tcactataat aaggagtcca
     7861 aataccaact agagggaaat cttccataga tatagttaca tatttactac catcattatt
     7921 acatatagat acttcatcta tgttggtgta tatcaaagca tcattcttga atacttctgg
     7981 atttaactgt aagtttttta attttccaat aggtattata tcatctgtaa aagaaccatt
     8041 taaattgatt ttttctacat catttttact tttaaactca aggtagtgtt tagaaaaatc
     8101 attttgttcg taaaaaggaa ggttgaatgc aggatgtcca cctatagaaa agaatatatc
     8161 tttagaatca gtatttttta caatccattc tacctcaata ctattatcat ttaatgtata
     8221 gtttatgatt agctcaaaag aatatggata cttctttaaa gtagattcat tatctgttaa
     8281 tttataagtt acaaatgtat ctcctttatc tacaatctca aagtccatat ctctagcaaa
     8341 cccatgttga gtcatgctat ataaattttc ttctattatt gtttcattat caaaaagttt
     8401 acctactata gggaacaata taggtgattg tctaccccaa tattttttat taccatccca
     8461 aagaatgttt ttgtcgtatt ttttagaaaa tattttagtt agttcagcac caaaactgct
     8521 tgattcgata attaaatttt cattttgtaa tatattcatt taaaatctcc tttattatat
     8581 tttatgagta tattatacta taaagtataa agattattaa attttatttg atattatatt
     8641 tgaaaggtag attttagtat attttgaaaa ttttgttttg aaaatttggg attatttaag
     8701 aaattttgta ttataaatag aaggaaatag ataaattgta tagaataaaa tatattaaga
     8761 taagttaata atgttaaatg ggaagtgagt ttatggaaaa accaaataca aaagttgtta
     8821 actttcaaga gtataaagac agaaaaaacc aaagagaagt aaatagagac gaacttttaa
     8881 agcttataaa aaaaattgtg acttcaaaat agggagttgt taaactccct attttaatgg
     8941 gtttcataat aattaagaca ttctgagtcc atctatataa tctttgaatt gtgagaaatc
     9001 tgattcacta cttaattcta tggtttctga accttccgta taaaaattct taagatagat
     9061 agtataatta ttatcaacac aatcatgata aatacatcta tatccatctt catacaaact
     9121 ttttaactta gaaaaatcat ccacaatatc atctccttaa agttctttta aatatatttt
     9181 tacaaaaagc atctaattgt atgcaaattg acaatgtaaa ataaaggaaa tgaacataaa
     9241 atgtagaata atttttatat gtatgtaaaa ataaaaagaa aaagttgaaa tttttagaaa
     9301 ataagagtat tttttatcta aaaagagagt ttaaaaaata aaaaaatgtt gggaggataa
     9361 aaaatgagtt tattagaaag tatatcaaaa aacatatatt ccctagataa ctcttctatt
     9421 gaaaaaacaa agcagagatt ggataggctt atacatccaa ctggaagctt aggaaagata
     9481 gaagatattt gcatgcaatt agcaggaata tttggcaatg aaaattttga cacaagtaaa
     9541 aaggttataa ttgcttttgc gggagaccat ggtgtatatg aagaaggcgt agcacctgac
     9601 cctcaaaata taacaaaatt acagtttcca aatttttcga aaggtctatg tggagttgga
     9661 gtaataagta aatttgtagg ggcagatgtt gtagctgtag atgtaggaat aaactgtgat
     9721 gaaaaactgg atggggtatt agactacaag ataagaaaag ggacatcaaa tatggcaaaa
     9781 ggtccagcta tgagtaaaca agaagcaatt agatgtttag agataggtat tgaaatagca
     9841 gagcagtgta tagaaaaaga ttataaggtt attggtattg gggagatggg tatagcaaat
     9901 actactccaa gtacagcaat aatatctgta atttcagggt gtgacccgtt agaagttact
     9961 ggtataggtg caggtcttaa gaaagaaaga ctaaagcata aggctgaagt aattagaaag
    10021 gctattgaaa ttaataatcc aaatccaact gatggagtag atattttatc taaagtagga
    10081 gggtttgaaa taggctcaat ggcaggtgtt atattaggtt gtagtgctaa taggattcca
    10141 gtagtaattg atgggtttat ttcttatgca gcagcattaa ttgcatataa gataaatcca
    10201 aaaactagag aatatatgat agcatctcat ttatcagcag aatcaggaac taaaagagca
    10261 ttagacatat taaaattaga ccctctatta aatatggata tgagattagg tgagggaagt
    10321 ggagctgctt tagcatttga tataatagaa gcttcaaact atacttataa aaatatggca
    10381 acttttgatg aaattgacat gggaagataa atataaaaat ttaatatgta ggagtgtttt
    10441 gatgagtaaa attattctag taacaggagg agctaggtca gggaagagta actttgcaga
    10501 aaaactatgt tttgacagaa ataataatac agcttatata gcaacctcta ttccatttga
    10561 tgatgaaatg aaagatagag taaaaaaaca tcaagagaat agacctagaa actggaatac
    10621 ttatgaaata tatgaagata tatattccat aattaaggag atatataaag aacatgaaac
    10681 tgttatacta gattgtgtaa cattattagt aaataatcta atgtttgaac acaatctaaa
    10741 tatagaagaa tctagtcaag aagaaataaa caaattagaa caatacataa aagaacaagt
    10801 tgtcaaactt attgaagaaa tagaaaaaac aaatctatac tttgtatttg taacgaatga
    10861 gttaggtatg ggaattgttc ctgggaataa acttagtaga atttatgctg atattgtagg
    10921 tcgtataaat caatatatag cttcgaaaag taatgaagtt tattttgtag ttagtggcat
    10981 agctatgaag ataaaggagt agtatgcaat tatgaaaaga tttattttaa tactacagtt
    11041 tttgacaagg atacctataa aattaaatgt aggatttgat gatgaatttt ataagtcaat
    11101 agtatatttt ccacttgtag gttttgtcat aggtatatta tcctatttaa taggatggat
    11161 ttctatgctt ttatttgaac catttatagc ttctataata ataactctag ctggtgtctt
    11221 aattactgga ggacttcata tagatggatt aggagataca tttgatgcaa tatatagtta
    11281 tagggataaa gaaaagatgc ttgaaataat gaaagattca agattaggta caaattcact
    11341 tctagcaatt atgtttgtac ttttattaaa ggttggattc gtttacgata taataagtaa
    11401 taattcattg tgggttataa tttttatgcc aatgattgca aggttaggag taatgttatt
    11461 gacatataaa actgtgacac caagagaaaa aggaatggga aatttattta taggaaaatt
    11521 aactacaagt atgttaataa ctgctataat atacacatta ttgatagttg ctcttattac
    11581 taaatttata tttttattac ctaatatagt gttgattaaa gtattaggtt cgattatagt
    11641 ggtttttgta tttataatat tattcaaaaa acatatttat aaaaaaattg acggagtaac
    11701 aggagatata ttaggctgtg gaatagaact ttcagagctt gtatatttaa tttacatata
    11761 tttgttaatt tttatgtttt tttaaagtaa aaagtggtga taatatgata agattaatat
    11821 taataagaca tgcactgaca aatgataata aaaaaggaag attatcaggc catataaata
    11881 gttgtattag tgaagagggc aaactacaaa taaataaaat taccagatat ctgagtaatg
    11941 aaaacataga taaggtttat acaacaccat ctacacgtac taaagataca gtagaaaaaa
    12001 tatcaaaatt gaaattatta gaaatagaag aaaaagaagc tttaagagaa attagttttg
    12061 gagactttga aggaagaact tttgaagaaa ttaaaatcaa atatccaaat gagtttgaaa
    12121 aaatgataaa agaaggtaac aactatagat atccaaatgg agaaagtttg atagactctt
    12181 ataaaagagt ggcaaaagaa attgataata taattttaga aaatgatagt aatttagata
    12241 caaagacaat acttatatgt tctcatgcag gaacaataag aaatataata acacatttga
    12301 tttctggtag ttataagtat cactggaact ttaaaattga taatgcatct attacagttt
    12361 tagaaattga tggtggattt gctgtaatag ataaaatgaa ttttacagac ttcatataat
    12421 ttaatcaaaa ccaaacatta caatctgtgt ttgtaatata tctgtaaaat tagcaatgaa
    12481 aatagaatat tagattaaaa ttaggttctt aattagatgt taaaagggaa aaaggttaga
    12541 tgcctttgca gcccccgcta ctgtgaaacc aacgaaacta taaataccac tgtcagtttg
    12601 atgggaaggt tatagaagta ggatgaagtt aagccaggat acctgcctaa tttaatttaa
    12661 aacaagaggt cttcggggtg aaagattttt aattaatata tataaaagtc ctatctttag
    12721 aagtattagg acttttttta atttgttttt tagtaaattt taacaaggag ggatggaaag
    12781 tggggaaaaa aataatgcta caaggaacag cttcaaatgt aggtaaaagt gtcttagtag
    12841 caggtttgtg tagaatattt aagcaagatg gttatactgt tgctcctttt aagtcacaaa
    12901 atatggcttt aaattctttt ataactaaag aaggattaga aatgggaaga gcacaagtat
    12961 ttcaagcaga ggctgcgaaa atagagccaa tagctgatat gaatccagtt ttattaaaac
    13021 cttctggaaa taataaaagt caagttattg taagaggtaa agttgtagga gaaatgcctt
    13081 cttctaagta tcatgattat aaattggagt tagtagatgt gttgaaggaa acatttaata
    13141 gcttaaattc aaggtatgat attgtagtta tggaaggggc aggaagtaat gcagagataa
    13201 acttaaaaga tagagatatc tctaatatgg gtatggcaga aatagctgat gcacctgtaa
    13261 taatagtcgg agatattgat agaggaggcg tatttgcctc attagcagga acaatgcttt
    13321 tattaaatga agatgagaaa agaagagtaa aaggtgtaat aatcaataaa ttcagaggaa
    13381 aaaaagagct tttacaagat ggtgtaaaga tgctagaaga catcataaaa gtacctgtta
    13441 taggagtagt accttataca gatataaaga tagaagatga agacagtgta actacgagat
    13501 ttaagaaaaa aatgaataaa agtgatatac acatagaaat aattagaact ccacatatgt
    13561 caaattttac agattttaat atatttgaga cacaagaaga tgtaagtatt agatatgtag
    13621 actatggaga atctttagga aatccagata tattaataat acctggaact aaaagtacta
    13681 tagacgattt aacatatata agaaaaagtg gtcttgaatc gcaaatcaaa aatttacata
    13741 gtcaaggtaa attgatattt gggatatgtg gtggatacca aatgttaggt aaaaaattaa
    13801 aagaccctta tcatgttgaa agtgaaattg aagaagttga tggaataggt cttttagata
    13861 cagaaacaac gtttgagaaa gaaaagacta caacacaagt agaagctact attgttgaat
    13921 gtattagtga atacatgaat aaccttaagg gaaaaaaagt aaaaggttat gaaattcata
    13981 tgggaattac aaagcgaggt aatgatgtaa gaagtttaga tttaataact aaaaaacttg
    14041 ataaagaagt gaattatact gagggaagtg taaataaaga ggggaatgta attgggactt
    14101 atcttcatgg aatatttgat gaaataagct ttacaagagc aattttaaac aatataagaa
    14161 agaaaaaagg tttaggagaa ttagaaagca aagtaaactc ttttaatgaa tttaaggata
    14221 aagagtatga taagttggct gattttttaa gagaacattt agatatagag aaaatatatg
    14281 agattatgaa ataatgatga cctttattta taatttaggt aggaggtgta ttatgaaaaa
    14341 aattttaatt gcgggtacta atagtggagt aggtaaaaca acaatatcat taggaataat
    14401 gcaagctttg gttaagagaa atatgaaagt acagccatat aaagtagggc cagactatat
    14461 agacccttca tatcacactt ttataactgg aagacattct agaaatttag attcttatat
    14521 gttagatgat gaaaaaataa agtatatagt aaataagtcc tcaaaagatg cagatatttc
    14581 agtaatagaa ggtgttatgg gcttatatga tggctttgga atagatttag ataattgtac
    14641 aagttcacat acttcaaagg tattaaagtc acctgttata ttagttataa atggtaaatc
    14701 tatggcagca tctagtgctg ctatggtact tggatataag gaactagata aagatgtaaa
    14761 tataaagggt gttatagtaa ataatgtaaa gactaatagc cattatcaaa taattaagag
    14821 ttctatagaa aaatattgta atgttgaagt attggggtat tttcctccaa ataacaaatt
    14881 ctctttggaa tctcgacatc taggattagt tcctagtgta gaaatgaaat cactaaggga
    14941 aaaattttat accttagcag atgaaattga acactatata gatattgaca gaataattga
    15001 aatatcagaa agcgatgaat ttgagagtga ttttgatttt caaactttaa tagaaaataa
    15061 taaaataaaa gaacatgtta ataataaatc aatagctata gcatatgata aagcattcaa
    15121 tttttattat agagaaaata tagagctttt agaggaactt ggtttagaaa taaactattt
    15181 tagccctctt gaagatgtat cagtgccaaa tgcagattat atttatattg gaggaggttt
    15241 tccagaaata tttggaaaag aattagaagc aaatgagtca atgagaactt ccatattaga
    15301 agcacataac aataatatac ctatttatgc agaatgtgga ggactgatgt acctaggtga
    15361 gaaactattg aatcaagaag aacaagaatt taatatggtt ggaatattta gtggaattag
    15421 taagatgaca tcttctttaa aaagatttgg gtactgtatt ggtgtagcca aggaaaatac
    15481 tcttttatct aaaaaagggg aatctattaa agggcatgag tttcatcatt caatatttga
    15541 aagtgatgaa aaatgtgtat attcaatgag aaaagaaaga gatggaaata tagtagaaga
    15601 gtgggatggt ggttatagta aaggaaacac tttggctaca tatcttcata ctcattttta
    15661 taataactta aattgtatag aaaatttttt gaaaaaagga aatgaataaa tgagtatttt
    15721 atctatttat ataggatatg tgacagactt aattgtagga gacccatatt cttttcctca
    15781 tccagtgaga tttattggta aacttataaa ttttactcaa ggtataataa gaaaggtctc
    15841 taaaaatgat aggcagttaa aattgggggg atttatatta tggtttataa ccgttggaat
    15901 aacatattta acaacttatg ctatagtgaa gttatttagt tttaatatat tattgagtat
    15961 tgtagtgaat agttttataa tttatacaac actagccaca aaatgtttaa aagacgaagc
    16021 attaaaaatt tataatgtat taaaaactga agatatagaa aaatctagga ttcagctatc
    16081 ttatatagta ggtagggata ctactaattt aagtgaacct gaaataataa gagcaactgt
    16141 agaaactgtt gcagaaaata ctgtagatgg tataattgcg cctatgtttt atgcatttat
    16201 tggaggagca ccacttgcta tggcctataa agctatcaat acattggact caacagttgg
    16261 atataaaaat aaaaagtata aagatattgg ttttgcatct gctaagattg atgatatagc
    16321 caattatata ccagctagaa tatctgtcat tcttatgaca attggaagtt tctttttaaa
    16381 ttataactat aggaactgtt ttaaaatctc tattagagat agaaagaatc ataaaagtcc
    16441 aaattgtgct ttttcagaag gtgcagtatc tggtgctcta ggaatacaac taggaggtac
    16501 caacgtttat tttggggaaa aagtatataa gccaacaata ggggataagc ttagggagat
    16561 agataaagaa gatataatta aaactaataa aattatgtat gcatcttcat ttgtatctat
    16621 gttggtattt acactaatat atgtgttatg taggatgatt ttaattaact taattatata
    16681 aattattgtt tgttaactta agggggacat agatgaagga tttagggcat ggtgccaatg
    16741 tagatgaaat ggcaagatta tatgggaaaa acccaaaaga aataattgat tttagttcaa
    16801 acataaatcc aaatgtatta ccaaatttag agagatacat tttaaaagga ttagaagagt
    16861 gtaggaatta tccagatata aattatacaa acttgagaga aaatatatca aaatatatag
    16921 atataaatcc agactttata atacctggca atggtgcaac agaagtgatt tatttgctta
    16981 tgaagagtat taaaaaaaaa ttggctataa taaatccgac tttttcagag tatagaagaa
    17041 gtgctgagtt aaataattta gatattatag atttagagtt agatttagaa aataacttta
    17101 aacttaatat agatataata aaagaaaata taaaaagatt tgatagttta tttatatgta
    17161 atcctaataa tccgagtggt aatgtccaag atttaaagga acttgttcat ttactagata
    17221 aacataataa ggtattaata attgatgaaa ctttcatgga atttgttgaa gatgaaagta
    17281 aatatagttt ggttaaatat atagaatcaa ataaaaatat atttataatt aaagctgtga
    17341 ctaagttttt tggtatgcct ggtttaaggc taggatatgg tcttacaagc aacactgaaa
    17401 ttatgaacaa aatttatgaa cacaaagagc catggactat caattctttt gcagatatac
    17461 tatccaattt tatttttgaa gataaagagt atattaaaaa tagtaaagaa tattacatag
    17521 aagaaagaga atatatgtta caagagttga gaaacataag aaatataaag gtctatgata
    17581 cagatgctaa ttttatcttg ataaaaatat ataaaaaaac aactaaagaa ttgaaaaaag
    17641 atttgtttaa gcaaggaaac atattagtta gagatgcgtc taattttata ggattagatg
    17701 atagttttat aagagtggct ataaaatctc atgaagataa caaaattctt atagaaaata
    17761 tgaaaaattt attgggtgat taaaatgaaa tcttatggaa tatgcccagc atcttgtgga
    17821 gaatttgtac aaggtataat tgatgatgaa gaatatttat gttcatatgc aatagatatg
    17881 tattctaaag tgtatattga agaaaaatta gtagatataa atttagggag atataagtca
    17941 aggttagcaa ttgaaaaggt atttgaaaaa tttaatttac ctaaaaaata tactaaaaat
    18001 atttctttaa atataaatag taaaatacct gttggaaaag gaatggcaag ttcaactgct
    18061 gatataggag ctactataaa ggcaacatta tcattgatag ataaagattt aagtagcgag
    18121 gagatttcta aattagcagc agaaatagaa ccaacagatt ctatatttat agataaaaat
    18181 agtatattta atcctctaaa tggaactgtt ataaaatacc taggaaatct gaccaatgcg
    18241 aaagttgtaa tacttgaacc aaacaaagta ctagatacta tgaaaattag attgaggcaa
    18301 gattataata aattaaaagt agagaataaa gaagtaataa aaaaatcatt tgctctttta
    18361 gaagaaggat tgaaaaaaaa taatttatct ctagttggag aggcttgtac tttgagtagc
    18421 ttagcaaatg agaatataga aaaaaaagag tatttaaatg aaataataaa aatatcaaaa
    18481 aaatatggag catatggagt aaatattgct cacagtggaa ctgtagtagg tattttaatt
    18541 gataagttta tgaatgataa aaagatgata gatgccttat gtgaatctaa cataaattct
    18601 gtctataata aaatttatac acaaaacata attaatggag gaattaaggg ggaaatagaa
    18661 tggaatacat aaagaatcct atgaaaatag aagaaaaaag ttttgaattg atacaagaaa
    18721 taatagacga aattagacca gattataaat ttaagaataa tatcgaagaa aaaataataa
    18781 aaagagctat acatactact gctgattttg attacttaga tatattaaaa atatcagaag
    18841 aagcagttga tagtataata gatgcattaa aaaataatgc aagtatttat actgatacaa
    18901 atatggctct tagtggtata aataaaagaa agttagaatc attaggatgt aagtataaat
    18961 gtttagtggc agatgatgaa actgtaaaat tagctaaaga aaaaggaatt actcgttcta
    19021 tggcagcagt agaaattgca gctaaagaag aaggaagaaa aatattcgtt ttaggtaatg
    19081 ctccaacagc tttatataaa gttatggaga tgaaatcaga aggtagatta gatttagatg
    19141 cagttatagg agttcctgta ggttttgtag gagcacaaga atcaaaagat gaagttgaaa
    19201 aaacagatat accatatata atttctaaag gtagaaaagg tggcagtaat ttagcagcag
    19261 caatagtaaa tgctatatta tatactatgt aggtacctat atggaagaat atgtatatat
    19321 agatggaaaa aaatatagaa gaggatatac tacaggttca tgtgcaacag gtgcatctaa
    19381 ggctgctgta tatatgttga ttacaaaaaa tagaattaat actataaata tagatactcc
    19441 taaggggata ccattactac taaaagttga taatataaat atttctgata catttgtgga
    19501 atgttccata aaaaaagatg gtggagatga tatagatgca actcatacca tggatattta
    19561 tgcaagagct gaaattgtag ctaaaaatga taagaacaaa gggtatctta ctctaaagga
    19621 tatagatagt ttaagcacca atagtgagtg taaaagtgag ctctataagt ttattagggt
    19681 ttatggcgga actggtattg gtgtggttac gaaaaaaggg cttagtgtgg atgttggtaa
    19741 gcctgctata aatccgactc cacttaaaat gataaatcat gaaataagaa agcttatagg
    19801 agataatttt gaatctatac ttggaaatga taaagtttta aaaataacaa tctttgctcc
    19861 acaaggagaa actgtagcaa aaaaaacatt taatccaaga ttaggtatag ttggaggaat
    19921 atctataata ggtacaactg ggattgttga acccatgagt gatgatggtt ggaagaagtc
    19981 tctatctata gaacttcaga tgaaaaaaga acaaggctta gataaaataa tattggttcc
    20041 aggaaatcat ggagaacagt ttataagaga aaaattaaac ttagatatta agtatgtagt
    20101 tagggtaagt aattttatag gctatatgat taaagaagct caaagaatag gatataaaaa
    20161 aatcttaatg gcaggccata taggtaaatt tataaaagta tctgctggta tttttaatac
    20221 tcatagtaag gtagcagatg ctagaagtga gatattagtg gcaaacttag ctttaatggg
    20281 agctagatac gaatttttaa ataaaataaa tcaatgcgta actactgaag aagctgtaga
    20341 gctaataaat aatagtgaat atagagaagt atataatata ttaagtaata aatgtaggga
    20401 gagagtaaag caatacttga atgaagattc agatgatatt gatgtggaag taatcatatt
    20461 ttcaatggat aagtctttat taggtaaatc ggataataca gatgatttag tggaggtctt
    20521 tatatgataa acataatagg tctaggacct ggtaacttag actatataac aaaaaaaggt
    20581 gaaaatttaa tttccacttc tgatgtatta attggtggta aaagaaattt agagtctata
    20641 aaaaattttg aaggagaaaa aatagttctg gattctaatt taagagaaat tatagagtac
    20701 ataaataaca ataaagaaaa gcaaatatca ataatagcat ctggggaccc attaatatat
    20761 gggataggta gatatctatc taaaaatatt gataataaga tgttaaacat ggtttctggg
    20821 attagttcat tacagtacat attttcaaaa atatatgtag atatgaatga tgtatatatt
    20881 acaagtagtc atggcaaagt accagatttt gattacatat tatcacataa aaaagtatgt
    20941 atggttacag attcaaagat aggaccaaaa caaataagta gagaaataat agatagaaat
    21001 ctaaataaaa ttattgttgt aggagaaaat ctgtcatatg acaatgaaaa aataacaata
    21061 gcaaaaccag aagagataat aagaatggac aattttgata tgaatgttgt ggtgatttta
    21121 gatgaggaat agtgaattta taacaggtaa agttccaatt actaaggaag aggttagagc
    21181 aatatcaata agtaagttaa atttagtaaa tgctaaaaac ctcatagaca taggggctgg
    21241 tacaggaagt gtaagtgttg aagctgcata taattatcct aatttaaagg ttatatctat
    21301 agagaaaaat gatgatgcta tagagcttat agagaaaaat aaaaaaaaat tcaacttaaa
    21361 aaatatagaa gtgataaaag gatatgctcc aattaatcta gggttaaatg ttaaagttga
    21421 gtctattttt cttgggggaa caggaaataa tctggaagaa ataatagaat ggtctaaaaa
    21481 aaatctcgtt acaggaggaa gacttgtagc taattttata ataatagata catttaatca
    21541 aactttaaaa ctgcttaaaa agcatggatt taaagaaata gatgtatgtg ttttgaatgt
    21601 ttctaaacta gagaaactag gaaaaggtga atattttaaa ccactaaatc ctatatatat
    21661 aatatcttgt gaaaaagggg aaaatgatga tgaataaagt acattttgta ggagcaggac
    21721 ctggagataa agagctgata acattaaaag gatacaaatt attaagtaat gcagatgtag
    21781 tgatatatgc aggttcactt gttaatcctg aacttttgga atactgtaaa gaagattgcc
    21841 aaatacataa tagtgcacat atggatttac aagagataat agatgttatg agagaaggta
    21901 tagaaaataa taaatctgtt gtaagactgc aaacaggtga tttttctata tatggttcaa
    21961 ttagagaaca agtggaagat ttaaataaat tgaatataga ttatgactgt actccaggag
    22021 taagttcttt tttaggggca gcatcatctt taggggttga atatacagtt ccagaaattt
    22081 ctcaaagtgt aattataaca agaatggaag gcagaactcc ggttccagag aaagaatcaa
    22141 tacaatcata tgctaagcat cagacatcta tggtaatatt tttatctgtt caagaaattg
    22201 agaaagttgt gtctaaatta ctggaaggtg gatatccaaa agatactcct atagcagtta
    22261 tatacaaagc aacttgggca gatgaaaaaa tagttaaagg aactttaagt gatatagctg
    22321 taaaagttaa agaaaacaac ataaataaga ctgctttaat aatggttggg agatttttag
    22381 gagaagagta taataattca aagttatatg ataaggattt caaacatgaa tatagagggt
    22441 aatattgcta taatctgtat tacagaaaat ggaaaaaaat tagctctaaa gataaattct
    22501 ctagtaaata actgtgttat atatcatgta aaaaataaaa aaagtaattt agttgttgaa
    22561 gaaacatgtg tgcatacagt tcaaaagaag ttaagtgatt ttgtaggaga aatatttagc
    22621 aaatttgatt acatagtatt tataatggct acaggtatag ttgtacgaag tatagcacca
    22681 tacataataa gtaagtttag tgaccctgca attttggtaa tggatgaaaa aggcaaaaac
    22741 gtaataagtt tattgagtgg tcatattgga ggggcaaaca gcttgacaca gtacataagt
    22801 agtcttattg atgctaatcc agtaataaca acagctactg atataaatca aaaagcttcc
    22861 ttagatatga tagcaaaaaa actaaatgca catattgata attttagaga aagtgtaaaa
    22921 gaagtaaatt ctatgctagt gaataataaa tgtgttggat tatatattga tgaaggatat
    22981 caagttgata ctaggggatt taaacttata gaaagtaagg atgagttaag agatagtttt
    23041 tcaaacacat atatagaaaa attagttgta gtaagcaata aaataaattg tattgaagat
    23101 attgagaatt taaacctaga taataaattt gaacattgct atagtaccaa aaaaataata
    23161 aaggtaattc caagagatat agtgatagga attggttgta gaagaaacac agatagtgaa
    23221 ctgatgtata attcacttga agagtttctt aataaacaaa atatagatat tagagcaata
    23281 aacacagttg gaagtataga actgaaaaaa gacgaaaaag caataattga attatcaaaa
    23341 atattgaagg tgccatttaa aacattttca gtagatgaaa tatctaaggt tgataatctt
    23401 tttgataaat ctgattttgt taaaaaaaat gtgggcgtgt attcagtagc agaacctgta
    23461 gcatatcttt tgagcaaagg taatttgtta gttgaaaaac ataaatataa aggaataact
    23521 ttttcagtgg ggagaataaa aatatgatat acgttgtagg aattggacca ggaagtaaag
    23581 atacaatgac tttagaagca ataaaagcaa tagaagattc tgaggctatt gttggatata
    23641 agacttatat aaaactaata gaagaattta tacaagataa agaaataatc caaaatggta
    23701 tgaggcaaga agttgataga tgtaaacaag cagtagagga agcaaaaaaa ggaaaaaaag
    23761 tagcagttgt aagcagtggt gatgctggaa tatatgggat ggcaggtctt attttagaat
    23821 taatatctaa agaagatgaa gatataaaag taaaggtaat tccaggtgtt actgcaagta
    23881 ttggtgcagc agctatactt ggagctccta ttatgcatga tttctgtcat ataagtttga
    23941 gtgatttaat gactccgtgg gaagtaattg aaaaaagatt gagattggcc gctgaagccg
    24001 attttgtaat ttgtttgtat aatccaagaa gtaagggaag aagtgaacat ctaagcaaag
    24061 catttaaaat aatgggtgag tttaaaggtg gagatacacc tgttggagtg gtaaaagatg
    24121 taggtagaga aaaagaagaa aagtttgtat gtacttttga aaatatggac tttgaaaaag
    24181 tcgatatgac tacaatggtt ataataggaa ataagtctac ctatattaat ggtgaattga
    24241 tgataactcc aagaggttat acagtatgat tttaatttta ggaggaactt cagatagttt
    24301 atctatatgt gataagataa atgaactgaa aaatcaacca tatatccttt cagttactac
    24361 agattatgga agggaacttg cacaacaaca tgcagagaat gtaattttag gtaagcttgg
    24421 aaaagaagat atgctaaaat ttataaaaga taataatata gtaaaaataa tagatgccac
    24481 tcatccttat gcagttgaag tatccaaaac tgctattact tgtgcaaaac tattgaatat
    24541 agattatata aggtttgaga gaaaatcttt aattgaagaa atagattatg ataataaatt
    24601 tatagtagat acaattgaag aagcctgtga aatagcaaat aaaataggga caaatatatt
    24661 cataggaaca ggaagtaaaa atttgaacag atttatagag gaaataagtg ataaaaattt
    24721 agttgttaga gttcttccaa cgagtgaagt catattaagt tgtgaaaaat taggtttaag
    24781 tgcagataat atagtagcta tgaaaggtcc atttagccag tatataaatg aagaaacata
    24841 taaacattac aatatagatt tagtcataac aaaagaaagt ggaatagctg gtgggttctt
    24901 agagaaggta aatgcatgta aaactttcaa tataccagta ataattataa aaagagaatc
    24961 gattaactac gaaaatgtat ttaatgacat aaataaaatt aaagacatac ttgtgtaatt
    25021 tttaggagga gaatatatga agaaagcctt attagtagtg agttttggta caagctatca
    25081 tgaaacgaga gaaaagacaa tagatatatg tgaagataaa ataaaaaatt cgttaaaaag
    25141 ccatgatttt tttagagctt atacatcaaa tatgataata aataaaataa aaaaaaggga
    25201 tggaatagaa atagataatc caatacaggc tttagataaa atatatgaac aaggatatga
    25261 tgaagtaata atccaaacac ttcatattat atgtggcgaa gaatttaaca aattaaaaga
    25321 acaagtagat ggatattctt ctaaattcaa aaagattgta ttaggaagac ctttgcttac
    25381 acatattgaa gattatcagg aagctgtaga ggcaattaag catcaaattc ctcatatgga
    25441 aagtaatgaa gcagtagtat ttatgggaca tggaacactt catgaatcac attcagcgta
    25501 tccagcattg gaatacatgc ttagggataa tggaataaat gcttatgttg gtacagttga
    25561 agggtatcca gaaatagaac atgtaataag aagattaaaa gaaggaaata taaaaactgt
    25621 aaatttgatg ccatttatgt tagttgcagg agaccatgct ataaatgata tggcaggaga
    25681 tgaagaagat tcttggaaaa caatattaga agaaaatgga tttaacgtaa gagttcattt
    25741 aaaaggacta ggagaaaatt cttatataca agataaattt gttcgtcatg ctgtaaaatg
    25801 tgcagagaat gcgaagttct atggtatagg agcagggcca ggagatggtt ctttacttac
    25861 gataaaagcg gttaatactt taaagaaact ggatattcta tacactccag aatcaaaaaa
    25921 gggtggagat agtctagctt tatcaatagt tagtgaatat ttacctgaat cagtggaaat
    25981 aaagtctagg catttcccaa tgagttttga tgggaatgaa aaaagtttag cttgggacaa
    26041 tgtggcacaa gaaattatat cagatgttaa tgatggtaaa aatgtaggat ttgttacatt
    26101 aggagaccct atgatttata gtacatatgt atatataatg aaaagaatat taggagaggt
    26161 agaagtcgaa actataccag gaatttcatc attttccaat atagcatcta atcagaattt
    26221 tccattagtc atggataaag aagctttagt ggtaattcct tgtactatgg aagatgataa
    26281 gatagagtat gctttgcaaa attataattc aatagtattg atgaaagtat ataaaaattt
    26341 taaagaaata ataaaaaaat tagaaaagta tgatttaata gaacatgcta ttttagtaag
    26401 taattcttcc caagaaagcg aaaatgtgtt tacagattta aaagaagcgc accttgaaga
    26461 taagatatct tatttttcta ctatactagt aaataaagat aataaaataa aataggtgaa
    26521 agcaatattt aaaggagact taatatgaat atagtggttg gaactagggg tagtaatcta
    26581 gcgcttatac aaacagaatg ggtaataaat gaattaaaaa agaagtatcc agaaattagt
    26641 tttgagatta aaatcataaa gacaaaagga gatttaattc aaaatgtttc actggataaa
    26701 ataggtgata aaggattgtt tgtcaaagaa atagagcaac aattattaga tggaaaaata
    26761 gacatagcag tacatagtat gaaagatatg ccttcttatt tagcagatgg actaaaattt
    26821 gctcatacgc ctaagagaga agacccaaga gatgttctaa tacttagaga aggatataaa
    26881 aatttagatg atttacctca tggagcagta ataggaacag gaagtaaaag aaggaagttt
    26941 caattattga aacaaagacc tgatttaaat atagtacaag ttagaggtaa tgtggaaact
    27001 aggatacgaa aaataaaaga tgaaaacatg catggtattg tgttggcagc atcaggaata
    27061 attagagcta atttgcaaga taaaataagt tcttatttac ctgttgatgt tgttattcca
    27121 gcacctgccc aaggagcttt agctatagaa attagaagca atgatagtgc gatagaggga
    27181 atagtaaatt cactgaagga tgagaataca gaaatccaaa tattggctga aagaggtttt
    27241 ttagatggtg taaatggaag ttgtcatatt ccaatggcag cttattgtga aataatccaa
    27301 gataaaatac atcttactgg cttatatgga gattcagaag gaaaaaaagt agtaatcaag
    27361 tctatagatg gagatatttc ttctcctaga gagttaggtt taaagttagc taaacttgta
    27421 ttgaaggagt atgagaatta tgaagggtaa ggtttactta gtaggggcag gaccaggtga
    27481 ttataaactc atgactttaa aaggtttaga gtgtataaga aaatcggatg taatagtata
    27541 tgatagatta gcaaatagta gttatttaag agaagctaag cctgattgtg aacttatata
    27601 tgtaggcaag gcatcaagta atcatatttt gccacaagaa gatataaata gaattatagc
    27661 agaaaaagct aaagaaggaa aaattgtaac tcgtcttaaa ggaggagacc cttatgtatt
    27721 tggcagaggg ggagaagaag cagagacttt agtagatgaa ggtatagaat ttgaagtagt
    27781 tccaggtata acttctgcta taggaggttt atgttatgcg ggtattccta taacacatag
    27841 agaccatgct tcgtcatttc atgtaataac aggtcatttg aagggtgatg atagtggaga
    27901 actaaactgg aatgctcttg caaataacaa aggtactctt gtgtttttga tgggaatttc
    27961 aaacctagag aaaataagtg aaaacttaat gaaagaagga aaagataagg atacacctgt
    28021 tgctcttata agttgggcaa ctagatataa tcaaagagtt gttacatcta cacttgaaaa
    28081 tgtttatgaa actgctatta aagaagaagt aaaaccgcca actcttatag tagttgggag
    28141 tgttgtagga cttagagaaa agttgaactt ctttgaaagt aagcctttat ttggaaaaag
    28201 tattgctgta actaggtcta gaaatcaaaa tagtgtattg gtagaaaaaa taatggattt
    28261 aggtggaaat cctatagaaa tacctactat taaggtagaa aaaatacaaa ataatattaa
    28321 cttagaaaat gaaataaaaa atataaataa gtataattat ctaatactaa ctagtaaaaa
    28381 tgccgtagaa atcttctttg aaaagatata tgaaatgaat tttgatttaa ggatattatc
    28441 taatttaaag gtatgtgcta taggttctgc tacatcaaat gagttgaaaa aaagaggaat
    28501 tatagcggat atagtaccaa aaaaatttgt agcagagtca ctgtatgaag agttagcgcc
    28561 tattttaaat gatgacgata atatacttat acctagagca gaaaatgcta gagattatct
    28621 ggttgacaaa cttaaggcta tttgtgaagt aacagaagta catatatata gaacagtaat
    28681 agatgaggag aaaaaaggtg agatattaga tattttaaat tctaatgaca tagattatat
    28741 aacatttact agttcctcaa cagtaaataa tcttgttgaa attataggaa aagaaaatat
    28801 taaaatatta gaaaaaataa aaacactatc tataggtcct ataactacag agacaataaa
    28861 tagtttggga ataaaattgt acaaagaatc tgctacatct actatagatt cgttagtaga
    28921 tttaattata aaaaattaat tttggaggct tgaatatgtt aagaagacca agaagactaa
    28981 gagcaagtaa agcaatcaga aatttggtaa gagaaactaa ggtgaatgta gaagacttaa
    29041 tatatccact gtttgtagta gagggggaaa atataaaaga agaaattaaa tctttaccag
    29101 atgtctatca tttatcaata gatatgctag aagacgaaat aaaagaaata agtgagttgg
    29161 gaattgaaca tataatgtta tttggtgttc cagatgaaaa agatgagtgt gcttcacaag
    29221 cttttaatga taatggaata gttcaaaagg caattagaaa ggtaaaagaa ataaattcag
    29281 atataaatgt aataacagat gtctgtatgt gccaatatac aagtcatgga cattgtggga
    29341 ttttaactga tactggttat gttgataatg ataaatcatt ggagtatttg gctaaaatat
    29401 ctgtaagcca tgctaaagca ggagctgata tggttgctcc atctgatatg atggatggaa
    29461 gaatacaagc aataagagaa gctttagatg aaaatggatt agaaaatgtt ggaatcatgg
    29521 cttatagtgt taagtatgct tcaaattatt atggaccttt tagagaggca gctcattcag
    29581 caccatcttt tggagataga aaaacatatc aaatggaccc agcaaatagc aatgaagcta
    29641 taatagagac tgaacttgat atattggaag gtgcagatat acttatggtt aaacctgcat
    29701 tatcatattt agatattata agaagagtta aagataattt tgacttacct ctagcagctt
    29761 ataatgtaag tggagaatac tctatgctta agtttgctgt gaaggaaggt ttattaaatg
    29821 aagatgtaat tcttgaatca attacatcta taaaaagagc aggagcagat ataataataa
    29881 cttattttgc taaagacatt gctaaactgt tgaaaaaata aattctagca gtataaagaa
    29941 tatcaaaaag tataggaatt gtaattaact attatacagt agttttgttt agacatttat
    30001 tgaagaataa tagaaactaa tttgcaattt ctatacttta tttatgtaac taaataaaat
    30061 ttggaggtag aagatgctat atcctattaa tttaaaatta gatgagttag atgtagttat
    30121 aattggtggt ggagaagttg catataggaa atgtaaaaat tttttagagt ttaacaaaaa
    30181 tgttactata gtttctaaac aaattttaaa taaattttat gatttaaaag gaaatataaa
    30241 aataataaaa gatgattata aagaaatata tataaaagat gcttctgtaa taattgctgc
    30301 cacaaataat agagagttaa atatggaaat aggattatat tgtaaagcaa aaaataagct
    30361 agtgaatgta gttgataata tagaaatatc gaactttact gttccatcat atataaaaag
    30421 aggagattta cttataagtg tgtctactgg gggcaagagt ccttcattat catcaaagat
    30481 aaaaaaagaa attgaagaga gatatactga agattatgag gagtatttaa atgtattagg
    30541 agatataaga aaaaaggtta taaaaaaata cgaagataaa agtaaaagaa aagatgtgct
    30601 taatatgttg gtaacacttg acctagaaga acttaagaag tttgatatat aaaaaataaa
    30661 ataaaaaatg ttaaaaaggt gttgacggat
//
LOCUS       DAMKMI010000033        30110 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMKMI010000033 DAMKMI010000000
VERSION     DAMKMI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30110
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.034"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            435..1127
                     /locus_tag="PC429_001427"
     CDS             435..1127
                     /locus_tag="PC429_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846942.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            1323..3383
                     /locus_tag="PC429_001428"
     CDS             1323..3383
                     /locus_tag="PC429_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3846943.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(3589..3912)
                     /locus_tag="PC429_001429"
     CDS             complement(3589..3912)
                     /locus_tag="PC429_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF3846944.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(3905..4606)
                     /locus_tag="PC429_001430"
     CDS             complement(3905..4606)
                     /locus_tag="PC429_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF3846945.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(4884..6269)
                     /locus_tag="PC429_001431"
     CDS             complement(4884..6269)
                     /locus_tag="PC429_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3846946.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALVEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(6715..7137)
                     /locus_tag="PC429_001432"
     CDS             complement(6715..7137)
                     /locus_tag="PC429_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF3846947.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            7615..10518
                     /locus_tag="PC429_001433"
     CDS             7615..10518
                     /locus_tag="PC429_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3846948.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISVSEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            10551..10997
                     /locus_tag="PC429_001434"
     CDS             10551..10997
                     /locus_tag="PC429_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846949.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            11120..11422
                     /locus_tag="PC429_001435"
     CDS             11120..11422
                     /locus_tag="PC429_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3846950.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            11603..13009
                     /locus_tag="PC429_001436"
     CDS             11603..13009
                     /locus_tag="PC429_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF3846951.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            13353..14411
                     /locus_tag="PC429_001437"
     CDS             13353..14411
                     /locus_tag="PC429_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF3846952.1"
                     /translation="MGDKMRASVLYSVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            14510..15196
                     /gene="araD"
                     /locus_tag="PC429_001438"
     CDS             14510..15196
                     /gene="araD"
                     /locus_tag="PC429_001438"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF3846953.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            15214..15861
                     /locus_tag="PC429_001439"
     CDS             15214..15861
                     /locus_tag="PC429_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF3846954.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(16034..17401)
                     /locus_tag="PC429_001440"
     CDS             complement(16034..17401)
                     /locus_tag="PC429_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF3846955.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(17591..17679)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            18107..20227
                     /locus_tag="PC429_001441"
     CDS             18107..20227
                     /locus_tag="PC429_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF3846956.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKTIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            20331..21584
                     /locus_tag="PC429_001442"
     CDS             20331..21584
                     /locus_tag="PC429_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF3846957.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSKMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(21737..22798)
                     /gene="ytvI"
                     /locus_tag="PC429_001443"
     CDS             complement(21737..22798)
                     /gene="ytvI"
                     /locus_tag="PC429_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF3846958.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            23072..23812
                     /locus_tag="PC429_001444"
     CDS             23072..23812
                     /locus_tag="PC429_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF3846959.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            23824..25602
                     /locus_tag="PC429_001445"
     CDS             23824..25602
                     /locus_tag="PC429_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3846960.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            25769..26686
                     /gene="pfkB"
                     /locus_tag="PC429_001446"
     CDS             25769..26686
                     /gene="pfkB"
                     /locus_tag="PC429_001446"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF3846961.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            26723..28642
                     /locus_tag="PC429_001447"
     CDS             26723..28642
                     /locus_tag="PC429_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3846962.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            29138..>30110
                     /locus_tag="PC429_001448"
     CDS             29138..>30110
                     /locus_tag="PC429_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3846963.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILISSLCVIVYSYKITSLYIFKNF
                     KIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVYIYPFLCEEYGWRSF
                     LQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAIANLIYGIGLSIFLG
                     YVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIFILLF"
BASE COUNT        11934 a         3255 c         4946 g         9975 t
ORIGIN      
        1 aatttaaata agtagctatt attgcagtga aatgaatgtt tctaagtata tttgggtgga
       61 agaatggtgg aagaaaatta gtgaaatgta attcttccac cattcttcca cccaaaaaat
      121 aaaaactgtt caataagaat taatatacat caattcagct gaatgcaatc aaaatactta
      181 aatgaaagta atctgaaaac aacataattc aaatcaattt attcaaataa acctcaattt
      241 atcttaaatc ttaagaaata tttaagaatt aggataatat tttcttaaga tttatatttt
      301 attatatagt tataagggaa agttacaaaa atgtaagcca aagtaatata acaatttagt
      361 cattgttgag aaagggagtg aaagatattg tataatatag ataagtatta ttatacaata
      421 gaggaagtga aaatatgtac aatatattag tagtagacga tgataaagaa attgttgatt
      481 cgatagaaat atatcttaaa tcagaaggat ttaaaatata taaagcatat gatggaatga
      541 aagctttgga aataataaca gagaatgaca ttcatctaat actaatggat ataatgatgc
      601 ctaaattaga tggaatcaaa gcaactgtaa aaatacgaga agaaagaaat attccaataa
      661 ttttaatttc agcaaaaagt gaagatacag ataaaataat aggattaaat attggagctg
      721 atgattatat aacaaagcca tttaatctat tagagcttat agctagggtt aaatcaaact
      781 taagacgata tgtaacttta ggtaattatg aagatacatg taaagaaatt ctaaaaagtg
      841 gagctttgga gttaaataca ctaacaaaag aagttaaaat tgatggtgaa aacataaaaa
      901 tgactccaat tgagtataaa ataattgaac ttcttttaga aaataaagga cgagttttct
      961 caatagatga gatatacgaa aaagtatgga atgaggaaag ttttaattca gaaaatacag
     1021 tagcagtaca tatcagacga ataagagaaa agattgaaat aaatccaaaa gaaccaagat
     1081 ttttaaaggt ggtgtgggga gttggttata agattgaaaa attttagtgg aaaaattata
     1141 aaagaaaaga gtttaatatt taattgcatt ttgattttag tcatttttac atttacacaa
     1201 atgttaggaa ttttaatatg ttttaatttt ttctctgaga gtttaattta ctctatgggg
     1261 attatgcttt tcttaaactt tattttatat aaaatactga caatgtagat gaggtgggca
     1321 acttggagaa atatagagta ttaaagttag tttcattaat cataatatct atctcatttc
     1381 cattaactat attgttggtg acaataggac ttgctgagcc taaatacaat atacaaaaaa
     1441 cttctacaaa gagtgggatt tttgatatat ctgatgattt ttatgatagt aatatatttg
     1501 acgaaggaac aatggcaagt ctattaccca agtttaagct atctgtaggt ggtaaattaa
     1561 ctagtaaaga aaaaggtaaa ttccaacaag aaggtaaaag tatagattta agtagtagtg
     1621 attctgagta tgataaaatc atgattaatg aacaagcttc tgaagaatat ttaagttatc
     1681 taaaaaatat aaaagtgtat gttattaata catacacagg agtattttat acaaatacgt
     1741 catatgctag tttatatgat tttaaacaaa aaactaagag ctattgtaat gtagagataa
     1801 taaataagat aggaaagagc tcatatatta aaacaataaa tggagaaaaa tttgaactga
     1861 ataatctgag tctggaacta aatgaagtag atgaaggttt tgaggcatat gtatcttttc
     1921 cagaagagcc aactatagaa gatggaatag tatatactaa ttttcaaata tttaaacaag
     1981 ctacagaaaa agtaagatta ttttttgtag ctagtttaag tttagcaatt atatgtttat
     2041 tatctataat tttatatatc aaaataaaag gtgaagtttt aaaaagaaat aatgtatttt
     2101 tactaatata cagtaagata cctatggaat ttaatgtaat attagtatct atttcaattg
     2161 caattttaac tatatatgct cttgctgtat cttatcatat tgatgtaatt gcattagttt
     2221 ctttatcttt actgatagta tcaaataata tattttttat aaatgtgcaa ttgagctatt
     2281 tagaaaataa aatgtgtata tttaagacaa gtattatagt aagagtgatt ttatggttaa
     2341 taaaaactgt aagggaggtc agtaacgcta taaagaaagt atctttagca aggaaagtta
     2401 ttgttatagt gataatatct ttagctttaa tcatggcaat tagcactcga agtataggta
     2461 tgggattact gttttctata tgtatttttt taggatttac aatatatata actaaaagac
     2521 tttcatatct tagttacgta atagaaggta cagaaagaat aaaaaaggga gaattagatt
     2581 ataagataaa aattactggg aatgataatt ttacgtcttt agctgaaaat ataaataata
     2641 ttggagaagg tcttgataga gctatagaag agcaagtaaa aagtgaaaga atgaagtctg
     2701 accttataac aaatgtatct catgatttga aaactccact tacttctata ataaattatg
     2761 tagatttaat taaaaaagaa gaatcaatac aaccagagta tataaatgat tatataaatg
     2821 tacttgagtc aaagtcaaaa agactaaaat tattgataga agatttattt gaagcgagta
     2881 gagtaagtag tggcaatata gaattacaaa ttagcaagat tgatttagtg caactattaa
     2941 gacaaagtat aggtgaactt gaagaaaaat tatctaaaaa taatttatat ttaaaattaa
     3001 atgttccaaa tgataaggtg tatatatggg cagatggaag aagaatgtat agagtatttg
     3061 aaaatctttt atctaatata gcaaaatatt ccttagaaga aactagagtc tatattgatg
     3121 tatatgacta tggtgaaaat gtaaaggtaa ctatgaaaaa tatatcttct tatgaattaa
     3181 attttgaccc aagtgagatt atggaaagat ttaaaagagc tgatgaatca aggaatactg
     3241 aaggaagtgg attagggctt gctattgcaa gagatttagt agctttacaa ggtggaaaat
     3301 tttatataga aatagatgga gatttattta aagcaaattt agagtttcaa aaatatgaag
     3361 aagaaataac aaatatggaa tgagatagta tttaagtata tatatttttt ttactgtcga
     3421 aagcttttat tataatatat tagaatattt acagtagaga atgaagagta aaaacagagt
     3481 aactttaagt cactctgttt tttatttaaa atcaataata taacaactta aattaaaatt
     3541 gtacacatag ttttaaaata acttatacaa taaaacatta tccaaatatc acaagataat
     3601 tccaaataaa tatatagata tcactcctgt aataatagaa ataccaatag atttaaactt
     3661 atatgcaaca agaagtacaa tcataaaaga tataatttta gggttagcta tagataaata
     3721 tagactatct ccattaaaaa atacatcttt agcagttaag gaagtaagta ttgctacagg
     3781 tataaatttc atgatttttt tcaaaaaatc aggcatctct ttttttgaaa aaattaacat
     3841 aggaagcact cttggtagat atgtaacaat gtacattcca attataataa aaaatatata
     3901 attattattc ataagattcc tcctttttac agtaagattc ttctataaag aaaccaatta
     3961 aagatgctaa aattgatgca actattatta ctaaattgct tttgaataat gcatataaaa
     4021 taacagaaat aatgattgat gatataaata ctacaatata aatcttattt tttatttgag
     4081 aaactattaa agctataaac atagaagtca atatgaagcc agaaatagaa ctgtctatag
     4141 atatactttc tcctaaaaat gctccaataa aattagctaa aactgatgaa attaagcaga
     4201 agaaattaag ataaatagca tttctatcag accaatttcc agcagtaaat ttttcataat
     4261 taactgcaaa agtttcatct gttatagtat gacaaaatgg caatataaat tttggacttt
     4321 tattttttat gtacatgttc aaattagaac tcattagaaa atgtcttaag ttaactataa
     4381 atgtagtcaa aacaatagat gttacacttg caccagtaga aatcattgaa gctgcaatga
     4441 attgagaact tcctccaaat actaaagtta ccattagtgc tatttgaaaa ggtgaaagac
     4501 cagatttttg taatagtatt cctcctacta tacctatagg tatataacct aagaatatag
     4561 gaattgaagc aataaagctt tcttttaatt gttgagttct ttccacaata aatcactcct
     4621 tttgtattaa atgtattaaa gtattagtaa aacagaaaaa tagattaatt gtataattgc
     4681 aattataatt aaaaataaat ttggattaat atataaaata ctatacaaca aatagataat
     4741 cttaaacggt tattaaattt tatctatttg ttattatgat gtaataatac atattaatct
     4801 ctataagtta attactatct ggtgtaattt tattttaaca acaaaaaaaa atacacacaa
     4861 ttaaaatatt gtgtgcaata caattatttt ttctcttcta atagtctttt taatacgctc
     4921 aaaccagctt taagttcttc tgtactttct gtagcacata cagctattct tattgcagaa
     4981 attggttttt cttttccaac agcaaaacgt tcagaagcat agacttgtac accttcttta
     5041 agtgcttgta tctcaaattt ttctgccatt attccatcag gtagaatcaa ccatctaaat
     5101 atacattctt catttcccaa aagattatat ccagataaat attgattaat aatcttgttt
     5161 cttcttcttg cagatttacg gcgtgcatta attaatttat cggcttcacc tgatgtaatc
     5221 atgcgataag caatttctgt taaaaaataa gattgagata gattaatgtt gtataatgcg
     5281 cttgataatg cctctcgata ctgtttaggt gtagagatat atgctagccg taaactaggt
     5341 gcaataattt ttgataaact cgtaatataa attgtttgat ttggcagata ggaagccact
     5401 ggatttaaat gtgtttcatt aagtaaacta tgaatagcat cctcaataac aattaaattg
     5461 tatttagatg caatatttgc aatcattttt cgtccatttt gtgacattat atgtgtagtt
     5521 ggattttgat aatctggtat tatgtataga cctttaatat tttcattttt acaagcatat
     5581 aatagtcctt cttcactaat ctcattatct tcttgtttaa ttggaattag ttgaatccca
     5641 agcattttag ctgctgtctt tattccagga taagttaatg ggtctacacc tattctatct
     5701 ccatgttgaa acaaaccagc taaaatagca acaattgcat tttgtccacc acttgcagga
     5761 agtaagctat ctgatgtagt ttcaagccca gctttataaa ttaatttggc aatagcttct
     5821 ttttgccatt tagcaccatc tctttgtcca tattggaata tagttttaaa atctatttcc
     5881 ttaattatgt gtttaaacaa agtattcatt tcttctagcg tgaaatctgg attcattgtg
     5941 cccatttcaa taaatgtaat tttattatta ctggacatca agaatagatt tgaacgtgta
     6001 tcataagata caaaagttcc acttcctgta actccactta ttagaccttt tttttcacaa
     6061 attttaaaag ctctagagat agtactcaca ttaacatcta agaaatctgc caattcacgt
     6121 tggggtggta atttagtacc tggtaataaa aatccattgt tgatatcttg ttctagttgt
     6181 tctacaagcg ccttatataa tatttgccct tttgaaggtc tctttggttt ccagttcata
     6241 ggataatttt caaaagaatt aattggcata agttttcacc tcacattatt aatttgattt
     6301 tttctttatc taaaacaata aattctaaag atacaaataa aaactgatag gaaataattc
     6361 tatcagtttt tgtcttttaa ttgatttcaa atcttcctat aatttttata ccttctttat
     6421 cataaagagg gattgttttt ttaaatatat taaaatttct ttttcttgta tataaaacag
     6481 cttcttctgg tgtctttggt tggttatcac aatcgtacaa atttcttttt tttaactaaa
     6541 agcaataata cataaaatta ttattgctaa tgatactctt aatgtggtat atagtcatag
     6601 tgtcacatgt ctatcaatag ttttttcagt aattattgta acgaatagta ggactcataa
     6661 aatacttaga aatatgtttt tagataaatt ttaaatatat aatctaactt atatctataa
     6721 attaacacta ttattctcca attccatttc tagttttttt ataaatttat taaattcttc
     6781 tggttgcctt tttccaaaca atactcttac aagagctgaa ttgatttttc caaatatatt
     6841 cctcttgctc tcactttcaa taaaaataag ttttgtattt tctaagtcaa tatgtactaa
     6901 ttcatatcta gtaatagtac tttctctttt attgcttgtt tttatctcat atactctatt
     6961 tttttcgaaa ttagttattt caaccttaaa tcttttatta tttttttgct gtacaaatgt
     7021 tcctactgct tttttctcat taaaattagg aaaatttagt cttgcagatt ttgtaaatat
     7081 attaaatatg tcttttgcag aatattttaa taaaacttca gttttaaatg tattcatatc
     7141 gttttccctt ctgtatataa atttaatatt tataagttaa tttttaagtt aaaatagtat
     7201 aatatgccat attaaatcat gttaaaacta tttatatagc taaaatctta cctatataag
     7261 tatgtatact atcatataaa aataaaatct taaaatagaa agggttaaaa acacagttta
     7321 ttataatttt tattgttgag tagatacgta ctattatata tgattgagca agtttttcaa
     7381 tcataaattt ataaagtatt ttgcattaaa atttaaacaa agaattaaat tatttaagat
     7441 tatgaataac taatacattt agtaaattac taaatataca aaatgtaaat acttatcaaa
     7501 atgtataaat acttatgaca tctagttact tacagggttt aagtagaaaa aaatatcaaa
     7561 gtaagtatgg catgatagtt gcttatatat ttatcaagaa aggagttatt aagcatggag
     7621 ttgagagata aacttgtaaa catcataaat acagaaaata aaaaagatcc attaacagat
     7681 gttcaaatag caaagttttt aagtacaact agagagaaca taactaattt gagaaaagaa
     7741 cttaatataa gtaattcaag gcaaagaaga tatccttatt taaaatcagc aatttcggca
     7801 atattacaaa agaataagaa tatttctgta tcagaaatta caagggaatt gatgacagaa
     7861 ggctttaata tatctagaag agttgtggag gaacttttac ctaaagaaag tttagaaatg
     7921 gatgtagtag aggaatctga agaagaatcg aatgacccat ttgaaactct gataggtaat
     7981 aaagggagtc ttagaaatgc tgtagagcag gctaaatctg ctatattgta tccaccaaaa
     8041 gggttaccaa cgttgattat tggggaaagt ggagttggta agagtttatt ttctagacat
     8101 atgtatgagt ttgcaaagca aaaaaagata gtaaaagaaa gctccaattt tgttgtattt
     8161 aactgtgctg attatagtga caatccacaa ttattattat ctttgttatt tggatataaa
     8221 aaaggtgcat ttacaggtgc tgaatatgat acaccaggtc ttgtagaaga agctgatgaa
     8281 ggagttttat ttttggatga aatacataga ttgcctccaa aaggccaaga aatactattt
     8341 tctattttag ataggggaaa gtttaggaga ttaggtgaaa ctaatgctga aagaaaagta
     8401 agtattatgt tgattggggc aactactgaa aatgtagaga ctaatttatt attaacattc
     8461 agaagaagga taccaatgct aataactttg ccaccattac atgatagatt actaaaagag
     8521 aaaatagacc ttatatacaa tatctttcaa caagagtgta atagaataaa tgcaaaaata
     8581 tttgtagata aaaatgtgat tgaaatactt gcactaaaaa agtttagtgg aaatatagga
     8641 caattacaaa atatgattca ggttctttgt gcaagagctt ttatgaagtt tataaattcc
     8701 aaagatgaag atagtgaatc gatagtagta gtagatataa atgaagtttt aaagcttaaa
     8761 gattctttta aagatgctgc ctttcaagag attgaatata cagagataag aaaatatttg
     8821 aaaaatgcta tatttatacc atttaattta gaagaatcat cagtaaaagg aagtctttta
     8881 agtgatgaat atatattacc agaggacata tacaaaacaa ttgaaaagaa atattatgac
     8941 ctaaaatccc tagacattga agatatagat atagagaata ttttatggac ttttatttta
     9001 aatagatttg ctgacttaaa gtttaatctc gatagtaatg aagaaatttt ttcaatgagt
     9061 gatttaaata gttttgtaag tgatagttta gttaaactgg taaaagaact tatgaatgat
     9121 atagttgaaa aaaatatgaa tttagaagta aataaaaata tgttcaaata tctagcaata
     9181 cacttagaag aagctataaa aagaattaag ctaaatcaaa agataataaa tgtaaattta
     9241 gaaaaaataa agattgactt ttcaaaagaa tatgaaatta gtaaaagttt tgcaataaaa
     9301 attgaggaat caattggtat taagattcca gatgatgaaa ttggatttat aacactctac
     9361 ataaagtctg ctctaaaaaa tgaagtgaaa aaagataaag tagggttaat aataatttct
     9421 catggacgta tagctacaga gactgtaaat gtagttaaag aacttttagg tgtaaagttt
     9481 cctgtggcta tagatatgcc tttagatgaa aaaccaataa atatatacaa taaggcagta
     9541 gaactgtcaa aaataataga ccaagggaaa ggcatattgt ttttggtgga tattggttca
     9601 cttacaaaca taggtcaaat agtaaataag agaaccaata taagcactaa aactattgat
     9661 agagtagacc tcctaatggc actagaagca actagaaaag tttctatggg agaggaagaa
     9721 cttgatgaaa tatttttttc tttgctaaaa gataggatgg gatataacta tgaattagaa
     9781 aaacacatag ataaatcaaa tgcaattgta acattatgtc ttactggaga aggaactgct
     9841 aagtatataa gtaaaacgtt ggaagaaaag tatgataaca ctaaatgtta tcaaatgagt
     9901 gcattggatg aaaatttatt ttgtaaaatt gaaaatctaa aagagacaaa caatatatta
     9961 gctattgttg ggacaataaa ccctaagata ccaggtataa attttatacc ctataataaa
    10021 gaacttttca aaaatttgga tatctatcta tcaaaatcaa aacaacaaga tgacagacca
    10081 gttaaatcgt atgaaagaat gcttgatgag gatttagtga tttttgaacc agatatatat
    10141 tttaaaaagg atttattaga atatgtatgt tcaattctta taaataaaga atatgtagaa
    10201 aaagattact tagattctgt tttacataga gaagaaatgc ttccaacata ttctaaagga
    10261 gctataggtg tgcctcatgg agattcttct actgtaaata gtacaaggtt tgtttttgtt
    10321 aaactaaaga atcctattga ttggggtgta ggtaatgtaa atttcatatt tatgccagtt
    10381 tttcaggcta atgataaaga gattgttaaa aatgttcttg agatactaaa agatgcagaa
    10441 tttatgaaca atgccaataa gtgttttgat aaagatgctt ttagaaggat tatatttgat
    10501 aaatttaaac atctgtagcc atagcaaaaa aattgagagg agttagagta atgaatgtag
    10561 atttaatatc attgggttta gaatctaatg attatgaaag tgttattgaa gaattaggaa
    10621 gtataatgtg taaaaaagaa tatgtaaaag aaacatacat aaatgctgtc ttggaaagag
    10681 aaaggacatt accaacaggt ctagatattg gagaaatgtg tgttgctatt ccacatacag
    10741 attcaaagca tgtaaatgag tctaatgtag ctgtagggat attgaaaaat cctgtaaagt
    10801 ttaattcaat gatagaccca aaagatagac ttgatgtaga acttgtattt ttattagcag
    10861 taaagaaccc agattcacaa gtaaaactgt taaaggattt aatgtcagtt tttcaaaata
    10921 taaagttatt aaaaaatata aaaaacgcat caacaaaaga agaagtagct aaattattgg
    10981 attttataga aatttaatgt aggagaattt tataaataaa aaattaaaat ttaaacaatc
    11041 aaagtaagta ttattttttt aataggttta atcttatata aaataaaaat ttagagtgaa
    11101 aaattaggag gaaattatta tgaaacaatt taatgtatta tcagtttgtg gttcagggac
    11161 agttacatca tctatggtag caggtaaatt aaaagaagta ctaggtaata aggggatttc
    11221 tattactaca acagaagcta gaccaacaga agcactaaat ttagcacaat caggaagatt
    11281 tgactttata acatttacga gtccacttca aacaggcgat tatggaatac caactataaa
    11341 tgcatttgct tgcctaactg gtatagggga agaggcattc tttgaagaag ttttgactgt
    11401 tattgaaggg atagataagt aagttttgga gacgtgaatt tatagtattt aaatcaatga
    11461 tttagatact ataataagct aatattaact aaatatttgt ggaaaaaaca ttttatatgt
    11521 tgtcataaat taaaaaatta taaaaatagt cacttaagta ttaaattaag cttataaata
    11581 tttataagta ggagggaaaa agatgttaga atttatgaaa tctataatcg atacatttgg
    11641 ttctgcaatt atagttccaa ttataatatt cataatagca aagatattta aagtaactac
    11701 taagaaatcg tttctttctg ctgtgtatgc aggagttgct ttacaaggtt ttacactgat
    11761 tttaaattca tttacaccaa taataacacc agttatcaat aggatggttg agagtacagg
    11821 agtaaaccta ccagtttttg atgttggttg gcaagcaaca tcattggttg cattttcaac
    11881 aagtgcaggt atgatatatt taggtcttgg tatattatta caaacaattt tatttttgat
    11941 aaaatggaca gacgtgtttc aaccatcaga tttatggaat aactattctt atatggtatg
    12001 gggagcaatg gttataggag taacaggaaa ttttccttta ggtatagcat gtatggtttt
    12061 attaaaccta tatagtttat taatatcaga gttagttgca aagagatggt ctagttacta
    12121 tagatatcct aattgtacaa taatagctat gcataatgtt gaagcttctg tgtttgcagt
    12181 atttgcagac cctatataca ataaattggg attaaataaa ataaaattaa atccaaaaga
    12241 attagagaag aaattagggt ttttaggtga accaattact ttaggtcttt ttttaggtat
    12301 gtttataggt atattaggta atatgacgag aataaatact atggaagctt ggggagaaat
    12361 aatgaaagta ggtatatcta caagtgcagt gatggcaatt ttccctaaag tagcaagtat
    12421 gtttgctcaa gcatttgcac ctataacaga agcagctaga aagattatgc aaaaagctgg
    12481 aaaccgtgag tggtacatag cagtaaatga tgctgttggg tatggtgaac ctgcaacttt
    12541 aatatcaggt cttatattaa ttccaataat gcttgtaata gctatggtac ttcctggtaa
    12601 taaggtatta ccagttgtgg atttattggc aattccatat atggttcaag gtttagttgc
    12661 catacataat ggtaatatac caaaagtatt ggtttcaggt ataatttggt ttggattagg
    12721 attatatgtt tgtacatcta cagcaccatt atttacagat atggctacaa atataggtgt
    12781 agctatacct gcaggagcaa tgttaataac aagctttaat atactaggaa aacctttgat
    12841 gggattagtt ttctttgcat ttctaagtgc aaatccaatt tatataggtc tttcagttgt
    12901 aatttacttt gttctatggg cattgtttag aaagaacaaa acaagtattc ttgattattt
    12961 agaaaaacaa gcacttaaaa atgtagaaga agagcctgta gcagtttaag tttataaaaa
    13021 tgaaataaga ataattaaag gagtttataa ttgattttat ataagtatca gataatacct
    13081 gagaaatatt taaatgaaat atagacttct ttttatttga taatatttgt aattataatg
    13141 cattgtatct ttatagttga gttttctgta taacctgcta tttaaaacat actcttttat
    13201 atttatacag taatgtggtt taattttata aaatgtaatc tctactggta aaagactttg
    13261 tatataaggc atttgcctga ataaaccatt tatcttttgt atggcataga tattgctata
    13321 tagaagtata tagcatgaaa ggagatattt atatgggaga taaaatgaga gcatcagtat
    13381 tgtatagtgt aggtgatgtc agatatgaaa tggtagatat accagaaata acagatacgc
    13441 aagtgcttgt aaatgtaaaa tatgttggga tatgtggttc agatttacct aggtctatgg
    13501 taagtggttt gagtggaaat acaaaatatc cattgatttt aggacatgaa ttttctggcg
    13561 aagttgttaa aattggagaa aaagtaaagc atattaatgt aggagataga gtagctgttg
    13621 caccattagt accttgtgga aaatgtgatt attgtaatga aggtaatttt ggcttgtgtg
    13681 atgattacaa tataattgga acaagagtaa atggagcgtt tgcagaatat gtaagagttc
    13741 cagaagaaca tattttaaaa ctaccagata cattggatta tgaaactgct gccggaattg
    13801 aaccagcaac tatagcttat catgggattt caaagtctaa tattagagta ggagatagtg
    13861 tagttgtgtt aggatgtggt ccaataggtc agtttgttat acaatgggca aaggtgtttg
    13921 gtgcatctaa gattatagca gttgatattt ttgatgaaaa attagaactt tctaaacttc
    13981 taggagcaaa ctatatactt aactcaaaag aagtaaatgt aatcaaagaa ataaaaaaaa
    14041 taacaaatgg tggtgctgat gttgtaatag agactgctgg tagccgattt acacaagaac
    14101 aatctctatt tgtagcaaaa aagaggggaa atatagtgtt tgtaggtata tcacacacag
    14161 aattaccact tagtgcagac gctactgagt gtatattacg tggagaactt accttgaaag
    14221 gttcttggaa ttcatataca agcccttatc ctggaagagc atggactgca acattggatt
    14281 ttatgggaaa aggtgacatt atttttaaac ccatgatttc acataaaata ggtcttaatg
    14341 aagtgggtga ctttttaagt aaaatgagca agagagaaat taatttcaat aaaatcctag
    14401 tagaaatata aaaatttaat aaaaaattct aaaagcaatt aaattttata actatgacat
    14461 taaataaaat aaaaataatt ttcttaaata aaatatagga ggaagaaaag tgttagaaaa
    14521 tttaaaagaa gaagtattaa aagcaaactt agaattacct aagaaaaagc taattactta
    14581 tacttgggga aatgtaagtg gaatagatag agaaaaagga ttagtggtga taaagccaag
    14641 tggtgtagag tatagtaata tgactgtaga agatatggtt gtagttgatt tagagggaaa
    14701 tatagttgat ggcaaattaa gaccttcttc agataccccg actcatctag ttttatataa
    14761 agagtttgaa gaattggggg gaatagtaca tacacattca agttgggcaa caatttgggc
    14821 tcaaatagga aaatctatac catcttgtgg tactactcat gcagattatt tttatggaag
    14881 tataccatgt accagaaaaa tgactagaga agaaatttgc aatgaatacg aaaaggaaac
    14941 tggaaatgta atcattgaag aatttatggg aagaaatcca attcattgtc caggaataat
    15001 tgtaaatgac cacggacctt ttacatgggg aaaagatgca aatgaggctg tacacaatgc
    15061 agtagtttta gaagaagtcg caaagatggc ttattataca gagttaatga gtcctgataa
    15121 tattatggat aaggttctta tgaataagca tttttcaaga aaacatggaa aaaatgcata
    15181 ctatggtcaa aaatgattat ctggaggtga attatgctag actctttaaa aggtaaaatt
    15241 gtggaaatag caattaaagc agagcattat ggtttatgta aagaaaagac tggtaatttt
    15301 agtattagag ataaggatac tggatatata cttataaccc catcaggtgt aggaagagaa
    15361 gaattaaaag ttgaacatat ttgtgtattg gatttggatg gaaatgtaat agaagttgag
    15421 aaaggtatta agccaagtag tgaggtattg atgcatattg aaatatacaa aacaagagaa
    15481 aatataaatg ccatatcaca tactcattct ttatatgcaa cagcattttc agtagcaaag
    15541 aaaccaatta taccaattgt atatgaatca gttaattatg gcggttatgt agatattgct
    15601 ccatatgaaa gaccaggaac tttaaaatta gctaagagtg tttcaaaatt actgatagat
    15661 acagatgcat gcttattaga aagacatggg ttgacaactg taggtaatga tttagacgaa
    15721 gcattattaa aatcaagata tgtggaagag gtagcagaaa tatactatag gtctttagta
    15781 ttaaatcaat ttaaagaact tgaagtagta gatgttgatg agtttaaatc ttggaaatac
    15841 ccagaagaaa taaagtttta atctaaataa ttttcattga agtggttaat taaaatatat
    15901 gtattattta tatcaattac tatgttttat tatgttaatt accgtatttt gcaaatatat
    15961 ttattacatt ttttcaaaaa aatatagaat ccgaaagcca tataaaattg acttgggatt
    16021 ctatactttt tatttatgca tttttcttta tctgattttc tggagaattt atatgttcaa
    16081 aatatttttt agtttcagat actattatag gtgataatgc aagtaaagct ataaggttag
    16141 gtattgccat aagcccattt acaatatcag caattatcca aacaacttct aactttaaga
    16201 aagcacctaa taacaccata gccacaaata cagttctata cccattcatg ccttttacac
    16261 caaacagaaa ttcaaaacat ctttctccat aataactcca tcccaaaatt gtagtaaatg
    16321 caaataatgt taaactaact gtaagtaata ttggtccaaa tgtaggaatt gctccgttaa
    16381 aagctgcttg tgtcataaca gctccattta ggtctgaaca ccacactcct gaaataacaa
    16441 gagagaatcc agtaagagta cagattataa tagtatctat aaaagtacct gtcatagata
    16501 taagaccttg ttccgctggc cattcagttt tagcagcagc agcagctatt ggagcactac
    16561 ctagaccaga ctcatttgaa aatacaccac gagcgatacc atttctaatt gccagtgaca
    16621 tagttgctcc taaaaaacca ccagtagcag cagtaggtgt aaatgcactt ttaattatta
    16681 acataaaagt ttcagggatt ttattccaaa aagatattaa aatgattcca cagattacaa
    16741 catatataac tgccataaaa ggtactattt tagtagctac attagatata cttttaagtc
    16801 ctccaaatac aaccaaagct acaaatactg ctaatacaac ccctgtaatt ctagggtcta
    16861 tcccgaaact attatttatt gcatctgtta ttgaatttac ttgagtaaaa gtaccgattc
    16921 ctaaaagtgc aactagtata ccacttatag cgaaaaatat tgcaagaggt ctccattttt
    16981 ctcccatacc atttactatg taatacattg gcccaccaga cacttggcca tttttatctt
    17041 tagttctgta ttttattgca agaacacctt ctgaatactt agtagccatt ccaaagaatg
    17101 ctgctatcca catccaaaaa agtgcaccag gtccaccagc tttgatagct gtagcaacac
    17161 caacaatatt accagtacca actgtagcag ctagagcagt acataaggct gcaaaacttg
    17221 aaacatcacc actacctttg ttttcagcag agaatattaa cttaagagct cttggcagtt
    17281 tagttatttg aacaactcca agcttaacag taagtagtat accagtacca actaatagta
    17341 ccagtagggg tggtccccaa ataaaagcat caactttgtt taaaatatca attagattca
    17401 taaaaacact ccctcatttt ttttgcttta cataatcaat agaattttta atgaagtaat
    17461 tgacaggaaa taagtataat ttattaataa tttctagtgt aataaaaaaa gctaaatcta
    17521 aaaaagattc agctctggat gtacactaat tagcaaccaa aataaaagta ttagtaaata
    17581 ctgtttttgg tagataatcc ctgtcctttt gcctgagaga ttgagcataa aagccttgcc
    17641 ccttcggcgc ccataatggg tctctccaga gttccgtaaa ttttacagtc ctacttttaa
    17701 aagtacctga gagtgttact ccttcggtgt gctatatagc aactcttcta taaaatctta
    17761 accggatatt gatttgtcat agcttattgt attaataata ttaatataag tttttatgtt
    17821 ttgtcaacta aaaaattaaa aaacaaagaa aaaatataaa aatgtaagcc tatttacgaa
    17881 ttataaactt agaaagatga gtaatatacg tgaaaatgaa ttttgtaata cacatacaaa
    17941 tgaagtgtaa aataagttct aaataaaaaa ataataaaat cttaaataat taattagata
    18001 tgattaaatt atggtaatat tatagttacg atattaagaa aagttctatt tagttagatt
    18061 ttaagcgtaa tatgagatta tgtatagaga aaggatgtat ttttagatgg gaaaaatatt
    18121 agtgctagca gaaaaacctt ctgttggaag agatatagca aaagttttag ggtcaaaaaa
    18181 tgaaaaaaat ggttatatag aggggccaaa gtatgtgata acttgggcac taggtcattt
    18241 ggttacactt gctgaccccg aaagttatgg agaaagatat aaatcatgga gcttagaaga
    18301 cctgccaata cttcccaagc accttaagac tgttgtaata aaaaagagtg gaaagcagtt
    18361 taatacagtt aaatcgcaaa tgaatagaaa tgatatagat gaaatagtta tagcaacaga
    18421 tgcaggtcgt gaaggagaac ttgtagcaag gtggataata gaaaaatcac aggtaaaaaa
    18481 aactataaaa cgtctttgga tttcttcaag tacagacaaa gcaataaaag acggatttgc
    18541 aaaattaaga agtggaaaag aatatgaaaa tttgtattac tcagcaatag cacgagcaga
    18601 agctgattgg attattggaa taaatgcaac cagagctttg acaacaaagt acaatgcaca
    18661 gctttcttgt ggaagagtac aaactcctac tcttgcaatg ttgcttaaaa gagaagaaga
    18721 aataagaaat ttcaaaccta aagagtatta tggattagaa ttaattgcta caaagggaaa
    18781 ttcagatata aaatttattt ggaatgataa aaacaataat tcttcaactt ttagtaaaga
    18841 aaagatagaa tccacattaa aaaaagtaaa aggtgtagac ataaaaatat ctgaagtaaa
    18901 caagtcatat aagaaaaaat attcacctgc actctatgat ttaacagaat tacaaagaga
    18961 tgcaaataaa atgtttggat tttcagcaaa agaaacttta tctataatgc aaaaactgta
    19021 tgaacatcat aaagtactta cttatcctag aactgattct agatatttaa catcagatat
    19081 tgttggaaca ttaaaagata gaataaaagc tgtaaatatt agtgattatt caaagacttg
    19141 taataaatta ttaaaaagca atattatagc taataaatcg tttgttgaca attcaaaggt
    19201 aacagaccat catgcaataa taccaacaga acagaaggta ttcatgagtg attttagtga
    19261 caaagagcga aaaatatata atcttgtagt aaaaagattt ttagcagtac tatctcctcc
    19321 ttttgaatat gagcagacaa ctataagtgc aactgtagaa ggagaaatat ttaaggctaa
    19381 gggaaacaaa gtaataaatt taggttggaa agaaagttat aaagatattg aagatgaaga
    19441 tgagtatgaa agtataccaa atattttaga aaaagatgta cttaaggtca agggattaaa
    19501 gataacttca ggaaaaacaa atccaccacc atacttaaat gagggaacat tattgactgc
    19561 tatggaaaag aatggtctag ggacagttgc tacaagagca gatataatag aaaaattatt
    19621 caattcattt ttaatggaaa agaaaaataa agatatatat ataacatcaa aaggaagaca
    19681 attattagat ttagttccag tagacttaaa gacaccagaa cttacagctt catgggaaaa
    19741 gaaattattg gatatttcaa agggaaaact tagtaaaaat acattcataa atgacatgaa
    19801 agattattca aaagatattg tactagaagt aaaaaatagt gataataaat ttaaacatga
    19861 taacttaact aaaaatagat gtccagaatg tggtaaattt atgttagagg taaatggtaa
    19921 aaaaggtaag atgttgattt gtgaagatag agagtgtaat actagaaaaa ctatatcaca
    19981 agtaaccaat tcaagatgtc ctaactgcca taaaaaatta gaacttcgtg gagaaggtga
    20041 ggggagaatc ttcacttgta gttgcggata cagagaaaag ttgtcgtctt tcaacaagag
    20101 aaagcaagaa gaaaaacaga aggcatcaaa aaaagatgtt aatcaatatt taaaaaatca
    20161 aaataaaaat caagagacat ttaataatcc atttgcagaa gcattagcta agctaaaaaa
    20221 caaataattt taaagttatt attatacaca attataactt taactgtata ttagttatat
    20281 gtactgatat aatatagtgt atagtttagg catgaaagta gggctgttaa atggatttaa
    20341 gtggcatatt taaatactat tgtaaagagt gtgaaaatac atggaataat tcgagtgttg
    20401 aattatttga gaatatagaa acgtatagta aagattcaca aaaaaagagg gaaaaagaat
    20461 tagataaatt gctaaataca atatcagttc atttagagag gtatccaagt gatgctgtat
    20521 tgagaaaaat gtgggtaaaa aagggcgagg ttttcttaca aaagacattg gaaaaagaaa
    20581 atatttttaa gttagaaaaa atggatgtag aggatagaaa aaaattttta gatataacaa
    20641 aacagtttat tagagatgct agaaaatttg atgatgattt acctataggt gatattatgc
    20701 aagctatgag gaatgtatgg atttcaaatg cattacaatt attatttggt aaagaagtat
    20761 attattcaaa agctaacttt gcatatagta tgttatatcc atatactgat aattatttag
    20821 acaatacaaa tatagataaa aatgataaga ttttatttaa taactggtta gaaaaaaggc
    20881 tcctgggaga acacattaaa tctaaggatt atcatgaaag taaagtatct aaaatgatag
    20941 attatattga aagtgtatac cctagagaaa agtttacaga agtttatgaa tcgttattat
    21001 taatatttaa aagtcaagta aatagtttaa aacaacatgg taaggaaaat catttgtgta
    21061 aagaagattt attatccatt tctattgaaa aaggaggttc atccgtttta gtagatggat
    21121 atttaataag tggattgatg acaaaggaag aaatagagtt ttgtatagga tatggatttt
    21181 tattacaaat atctgatgat ttacaagata taaaagaaga tttaaaatac aaccataaaa
    21241 ctattattac agagatgtca aaagagggta ctttagataa agttgtaaat aaactaataa
    21301 attttactat tgagttaata gatagtttta aaattaataa taaaaataaa tctgtaataa
    21361 ctatgataaa gaatgattgc ttaatgttaa ttttattttc tgtagtttat aatgctgaat
    21421 ttttttctgt aggatatata aaagaagtag agaaatttat tccatataca atagattatt
    21481 cattagagat tgaagaaaaa ataaaagaaa aatttaaaaa tatagatgtt ttaaataatg
    21541 aaaatgaata taaagaaatg attgatatta tttgtgcaga gtagaaaaaa agggagtgtc
    21601 ttagaataga ctttttagtt tatgaggcac tctttttgta taaaatcaaa tgtattttca
    21661 tcattttaat aattaaaaag agcttatccc tatttgagaa agcccttttt aatttaataa
    21721 aaatgtcaag gttaaactat ttaaatgcag taataatccc attatcatgt aagtttttta
    21781 tgattgtagc tgcgataggg aatccaaata atccagctat tccaaatagt tgagctccaa
    21841 caaacataca cattaatgtt attaaaggat ataggccaat ttggtgtcct actacctttg
    21901 gttctaatat ttgtctaaca acggttataa ttatgtatag tattaaaagt cctattgcta
    21961 aattaatatt tccattaatg agtgagataa tcatccaagg tgtcaaaata cttcctgtac
    22021 ccaagactgg caaaatatca acaagtgcta tgatgacaga tatagtaaat gcattttcaa
    22081 cctttaggat agtaagtcca ataagtagtt ctatgaatgt cactgatagt agtattgcat
    22141 aggcttttag tagttttaaa agagcattaa tactgctatt ttttatattt attatcataa
    22201 attgactttt ttctggaaat tgttttaaga taaagtttac tattttttga tagtcaaaag
    22261 taaagaaaaa agaagcaatt aaagtaataa taagttttaa cataaatgca ggtgttttac
    22321 ttgcaatact agcaatagca ttgagtgcat ttgttgaaat agaagctact aaagacatca
    22381 tagattgact tatattatcc caaccaagta ttaatgctag gttaacatta ggtgtactag
    22441 catctatctt tgaaaataaa gtatttagcg ttggttgtat acttgattta tatatttcag
    22501 gaagtctaaa aaatacatca cttacacttg caaatatctt cacaccaaag ctaactaata
    22561 aaaatataca caacccataa aaaattaaaa gtattactat agaaataagt gtacgattta
    22621 aatttgtatt atttgtaata agtcttataa taggtcttaa gatggatgca atgataaaac
    22681 tgattacaaa aggcattaat aatggcaata agaattttaa tacaatatat acaatacttg
    22741 ttattaaaac tacatatgta aatttaatta taaaattctt ttgcttttga atatccataa
    22801 tataaaacct ccaatgtatt tgatattatt ctaaatatgg tcttttttac agaaaatata
    22861 ctactgtata ttgattataa actataaagc ttatgtaaag taaataaatt atgtaaaatt
    22921 taggttaatt ttgtataaag agatactttt atattttaca attaataacc aaatactaaa
    22981 aaatatttta aataaagaac ttaaaattgt gataaaatta atttatagat attaaaaaaa
    23041 gtataatata tggtttttaa gaggtgctaa gatgaaaaat tttagagatt taggtgggaa
    23101 caaaactgag gatggcagaa ctgtaaagaa gggattattt tataggtctg caaaattatc
    23161 aaatctaagt gaaaatgata taaagatttt aaaagagtta aatattaaat atatatttga
    23221 ttatagaagt gatgaagaag ctcgtaaaca tccatcaaca ataatatcaa atataaaaaa
    23281 tataagaata ccagcaatga gagagctaga agaatcagga ggaagttttg gttctattga
    23341 agatatgatt gatggattgt ttgaaaaaga tggtgcattt aatatgttaa ataatagtta
    23401 ctataatttg cctataaata atccgtcata taaaaaatta gtagaactta taagagacta
    23461 ttctaattta ccaatattaa atcattgtac agctggtaag gatagaactg gtgtggggag
    23521 tgctataatt cttatgattt taggggtatc gagagagaat ataatgaaag attatctaaa
    23581 aagtaatgat tttgcagata aagagataga aagatttata gaatataagc ctaaatttaa
    23641 ggacattcca aaagaaaatt tgaaatatat atttggtgta aatgaagagt atatgaaaac
    23701 tgcatttaga agaattgatg aagaatacat tagtgtagaa gcatatttat atggagaatt
    23761 taatttaaat aaagaagaaa taagaaaatt aagaaatcaa tatctagagt aaaaggtgag
    23821 tttatgagtt taaatataac agcaagacag ataaatataa taaaaatatt gttaaattca
    23881 aaagatacaa ttagtggtat tgccttaagt caagagatag gatgttcttc aaaaactata
    23941 caaaatgaaa taaaggatat taataaacaa cttagaaatg gtaaaatttt gtcaataaga
    24001 ggagttggtt ataaactaga aggaagtttt gatgaggtaa atctaaatag caatctctat
    24061 gatgatgtag atagagtaga atatataatc agaaaaatat taaccttaag taatgaagaa
    24121 aaaaatacta taaaacttga agatttagcc gattctatgt atgtaagtgt ttcaacagta
    24181 aaaaatgatt taaaagaagt aaaaagaatg ttggaaaagt attcaatatc agttaaatct
    24241 aagcataaac aaggtatatg tgttttagaa agtgaagata agatattaaa ttgtatagtt
    24301 gacttaagca ataagagaga taatcaattg agtttaaatg attttttaaa tgatgatata
    24361 aaggaaagaa aactattaat aaaaaaatta ttattagaca tattaaatga aaatggatta
    24421 gtattaactg atatagaatt taagagtgtg ttaaataata tattagttat gttaagcaga
    24481 cataagtata atgaaaaaga atttatagag aattatatag aaaattatag gagcaaaaga
    24541 agctcaattt tagaaaatga acaaaacaaa gaaattataa taaattcaat aaaaacattt
    24601 tgtaaaaatt taaaattagc tacatcaata gatataagta atgataaggt atttgaagag
    24661 tttttatata agcatattag tagtttatgt aaaaaaataa agttaggtat aaatcaatca
    24721 cctataatgg ctcaagatat aaaaataaaa tatccatttg cttttgaact agctaatata
    24781 gctaaaaaga caatagaaaa agatttgaag atagaaatta atgaagatga agttgcaaac
    24841 atagctctac atataggagg agcagttgaa agagcatctt ataataataa agataaggtt
    24901 ttcaaaacta taatagtttg tacttcaggc ataggcacat caatgttaat aaaagctaaa
    24961 ttagagaata tatttaagga aaaattagaa atactaaaga ttattccttc atacttagtt
    25021 gaatatataa aagttataga tgttgatttt gtaatatcta ctgttcctat tgaagtagga
    25081 gatgtgccag taataaatat atctccaatt ttaagtgaga aagaaattag attaattgaa
    25141 aaatatatag aaacaggaaa tatataccta gatttagata taaaaagcct gtttgatagt
    25201 gaattgtttt ttactgattt ggtgtttaaa aggaaagaag aagttataga ttttatggca
    25261 agtaaacttg tacaaaaggg ctatatagat gaggatatga aaaaatctta ctttgagaga
    25321 gaaaaaatag caacaacaga aatagggaat atggtaacta taccacatgg agctattgga
    25381 aaaatttttg ataataaaat agcaattggt atattaaaga agcctatttc ttgggaaata
    25441 tcagatgttc gtttaataat aatgttagcc cttgataaag ataaaatatt agattatgaa
    25501 gtaatatttt caaaaatata taaaagagta gattctatag caaaagttat aagtatatgt
    25561 gaaaatagta gttttgaaaa atttgtaaat ttatttaatt agatgggtag tattgtatat
    25621 acacctgtaa atttggataa tataaaacta aataagataa aaacgataaa tttctctttt
    25681 aattaagaga aattttttga ctttcaagga tattaatata aagttaagat ataaacataa
    25741 agcaaataaa taaaaaagag gtggcaatat gatttataca gtgacattaa atccatcaat
    25801 agattatata gttaagctag atgagctaaa gacaggaagt acaaatcgtg taaaagaaga
    25861 atacgtttat ccaggaggaa aaggtataaa tgtatctcgc atacttaagg aacttggaaa
    25921 tgataataca tctttaggtt ttataagtgg ttttactgga gaatatataa taagaacatt
    25981 ggaagaaaaa gaattgaaga cagattttat aaagataaaa agtggttttt caagaataaa
    26041 tgtaaaaata aaggcgttaa aggaaactga gattaatgga caaggtccaa atatagatga
    26101 tgaagacatt gatactttat ataaaaaact tgataagcta aatcaagatg atattttaat
    26161 attggcagga agtataccat ctactttaga tgaaaaacta tacgaaaata taatggctag
    26221 attagaaaaa aagaatataa aagttgtagt agatgcaact aaaaatcttc ttcttaatgt
    26281 tttaaaatac aaaccttttc taattaagcc taataacgac gaattagaag aactatttgg
    26341 tgttaaatta aattcaattg aagacatggt aaaatatgct agaaggttaa aggaaatggg
    26401 agctataaat gtcttggtat caatgggaaa agatggagca ttgttgatta cagaggaaga
    26461 tgaagtgctt ataagtgatg ttccaaaagg aaaagtaaaa aattctgtag gagcaggtga
    26521 ttcaatggta gcaggattta tatctgggta tttgaatact gggaaatatg attatgctct
    26581 taaattggga gcagcttcag gtagtgcaac tgctttttca tatgacttag ccaaaagaga
    26641 atacattgat aaattggtaa atgaaatatc tgtaaagaag ttttaaaata ttaaaaaata
    26701 tatagaaagg taattaatat ttatgaaaat tatagattta ttagatgaaa agtcaattaa
    26761 gttaaattta aattcaaaaa ccaaatcaga agcaatagaa gaattagtag atttggtcgc
    26821 aaatagtgga aatttaaatg ataaagaaaa ctacaaaaaa gcaattttgg ctcgtgaaga
    26881 aatgagtaca acaggaattg gagaaggtgt agctatacct catgctaaaa attcttcagt
    26941 tactaaagct tgtatagcag cagcagtgtc aaaagaaggt atagattatg aatcgtttga
    27001 cggaagttta tcaaatctat tttttatgat agctgcacca gacggagcta acaatactca
    27061 tcttgaagta ttatcaagat tatctacaat acttatggat gaagacttta gaaataaact
    27121 tataaattca agctctgaaa aagaatttaa ggaaataata gataaaaagg aaagagaaaa
    27181 attcagtgaa gaatatcaag atgagaaagt ggctgaaaaa aatataatag atactaaaga
    27241 gaatgatgct aataagtatc ctaaggtttt agcagtaaca gcatgtccaa ctggaatagc
    27301 acatacattt atggcagctg aaagtttaaa taaaatggca gaaaacaaag gtgtatcaat
    27361 aaaagtagaa actaatggtt cagcaggtgt taaaaataag cttacaaaag atgaaataga
    27421 aaatgcaaca tgtataatag tggcagcaga taaaaatgtt gagatggcta gatttaatgg
    27481 caaaaaagtc ataaaaacta aggtggctga tggtatacac aaagcggaag aacttataga
    27541 caaggctgta aatggagatg ctccaattta ccatggagga gatggaagtc acaacgaatc
    27601 aaatgaagaa agtgaaagtg gatttagaaa agtatacaaa cacttaatga atggtgtatc
    27661 taatatgtta ccatttgtta ttggtggagg gatacttata gcgatagcct ttttacttga
    27721 tgattatact attaaccctt caaactttgg ttcaaataca cctatagcag cattttttaa
    27781 aggaatagga gataaagctt ttggatttat gttacctgta cttgctggat atatagcata
    27841 ctctatatct gatagaccag cgtttgttgt aggatttgta ggaggtgctc tagcaggtga
    27901 tggtggttca ggctttttag gtgcactact tgcaggtttt atagcaggtt atttagtaga
    27961 agggcttaag aaaatattta gtgtactacc agcttcattg gaaggaataa aaccagtatt
    28021 gttatatcct ttacttggaa cacttttgat gggaattata atgacatttt tagtaatacc
    28081 tccagttaca gcaattaata atgcaatggt tggattttta aatggtttag gtggaacttc
    28141 aaaaatagtt ttaggattag tattaggtgg aatgatggct gtggatatgg gtggacctgt
    28201 aaataaagca gcatatgtat ttggagttgc atcacttgaa tcagggcagt ttgaaataat
    28261 ggcagcagta atggctggtg gtatggttcc gccacttgct atagctcttg caactacatt
    28321 ttttaagaat agatttacta aagaagaaag agattctggt aaagttaatt atgtaatggg
    28381 attatcattt gttactgaag gagctatacc ttttgcagct ggagacccac ttcatgttat
    28441 accagcttgt gttggaggtt cagcagtggc aggagcactt agtatgttgt tcaacgcagc
    28501 tcttcgtgca cctcatggag gagtatttgt tataccagtg gttacccatc catttgcata
    28561 tatattagct atagctgtag gtgctttggt tggaatgatg cttttagcat tacttaaaaa
    28621 accattaaat caagaagtat agcaattaaa ggatattcaa gaacttaaaa tatgatgttt
    28681 tataaaaatt aatatattgc tgaattattt taaaataagg attttatgag gctgttatat
    28741 aacgatttgt aattgttatc ttgacagtct tttattttta tataagtaaa ataagtgtgt
    28801 tttgctttat ttgtatttaa attgttttta agttagaata taaagaaaat tatatattat
    28861 tttaatattc actcgaacta gaaaataata tttgttgtat tttttgtata taaaaatata
    28921 ttgtaatatt aaaaaaataa tagtaaactg attgcataaa aatgattttt atataaattt
    28981 taaacagaag aataaatttc taacgaaatt ggatatttct tctgtaaata catattcaaa
    29041 aaaagaatta taagagttta gtcagcagtc gtgtaagtaa taaggacttt ataaatatat
    29101 agaaatataa tgatatgagt ataagtagaa aaattttatg atttttacaa ttatttttta
    29161 taaaaaaggg aggttatgtt tgttgaattt acaatcaaaa tgggtaaact ttttaaagcc
    29221 aacatatgtc agtgatgaaa taaaaagaaa aaaactacaa gttataatat ttttttctat
    29281 aacttatggt ttatcttatt cactaggttt attagcaata tgttttaatc atttaataga
    29341 ttcagaaatt ttagctcaat ttatgatgat attgccatta tcttcagttt ctattgcaaa
    29401 attttacaca gagggaagaa ataatgataa atataatttc tattcatcag taatattatt
    29461 ttttattatt tactttttta tatttattat tgaatcatta aagttaataa gtattaatca
    29521 atttcaatta atgtatctta tgttaatttt aataagtagt ttatgtgtta ttgtttattc
    29581 ttataaaata acaagtttat atatttttaa aaattttaag ataggtattt tgctattttt
    29641 ttactttttg ttttctcaaa ttatttttgg attaataata agtgataatc aatttgatta
    29701 taagaatttc ttgaattata ttcttcttcc tataatatca ttagtataca tttatccttt
    29761 tttatgtgaa gagtatggtt ggagatcttt tttgcaaagt attttatttg atagatttgg
    29821 caaaaaaata gggattgtaa tggtaggcac ttgttggagt ttatggcact tacctttaca
    29881 atttacacta tatagtcctc aaactccaat aattggagct atagccaatt tgatatatgg
    29941 tattggactt tctatatttt taggttatgt ttatatgaaa actaagaaca tatggttttg
    30001 ctctataatg catgttctaa ttaatagctt aggattaatt tttaatgatt ctgaaataat
    30061 tattaattac catagtatta tacaaagatt gatatttata ctattatttt
//
LOCUS       DAMKMI010000034        29540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMKMI010000034 DAMKMI010000000
VERSION     DAMKMI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.035"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..92)
                     /locus_tag="PC429_001449"
     CDS             complement(<1..92)
                     /locus_tag="PC429_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846964.1"
                     /translation="MKDKWILCPVCGNKTRLKIRADTELKNFPL"
     gene            complement(131..418)
                     /locus_tag="PC429_001450"
     CDS             complement(131..418)
                     /locus_tag="PC429_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846965.1"
                     /translation="MSTLIIGFIVAGVLLIAEYLLCTKFRSPLWGGIIPLLILAGTIY
                     VFASGRIPFERPYIFPFVVVNTLFFGDWGTGRDKYKKIKQTEMEKMKAKDI"
     gene            complement(421..717)
                     /locus_tag="PC429_001451"
     CDS             complement(421..717)
                     /locus_tag="PC429_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HDF3846966.1"
                     /translation="MELDKIPQWILALEQEDATFLKNFALKSGSLKEIAKMYGVSYPT
                     VRLRLDKLIQKIEMCDQQEEEPFQTFIKGLAVDSRIDVETAKIIIEKYKKEKEK"
     gene            complement(806..1711)
                     /locus_tag="PC429_001452"
     CDS             complement(806..1711)
                     /locus_tag="PC429_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3846967.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKSVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQALNTDTIRKDIPVSSAVNGVQIWSVTQADENQFSVIFTVEQLITENGSTKT
                     VRSAYEVTVYVDGSGNMVLTKNPTITSIPVKSGYTPKAKESDGTVDAVTTDEINEFLT
                     TFFKLYPTATAKELSYYVNEGVLKPIGKDYVFSELVNPIYNRIDSQVTVSVAVKYLDQ
                     QTKATQVSQFDLVLEKSGSNWKIIK"
     gene            complement(1731..1838)
                     /locus_tag="PC429_001453"
                     /pseudo
     CDS             complement(1731..1838)
                     /locus_tag="PC429_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010989669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            complement(1956..3752)
                     /locus_tag="PC429_001454"
     CDS             complement(1956..3752)
                     /locus_tag="PC429_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDF3846968.1"
                     /translation="MKPTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPARSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIINQKIKDARFLKLIRSFLKAGYMEDWKYHKTYSGCPQGGIISP
                     ILANIYLNELDRHVMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKSATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTVIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTESDRYEIHHVNKVKNLKG
                     KSEWEKIMIAKRRKTIVVCHKCHMAIHHGEKK"
     gene            complement(4406..5305)
                     /locus_tag="PC429_001455"
                     /pseudo
     CDS             complement(4406..5305)
                     /locus_tag="PC429_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
     gene            complement(5302..7494)
                     /locus_tag="PC429_001456"
     CDS             complement(5302..7494)
                     /locus_tag="PC429_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF3846969.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPENEDGKTREEVVKTEIESNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDIPTQTAYAVHSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPVTASA
                     IPKTNVEKTQEVKRPATTSTSKAGEPVKTNSVKERPLSSGTSDGKIPQSAQTVHSKNV
                     EKVVSHETHQNYKAERTTKAQTVEQAKRTTEHTEKNRNLVTKKGQKKK"
     gene            complement(7494..9944)
                     /locus_tag="PC429_001457"
     CDS             complement(7494..9944)
                     /locus_tag="PC429_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3846970.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            complement(9931..10320)
                     /locus_tag="PC429_001458"
     CDS             complement(9931..10320)
                     /locus_tag="PC429_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3846971.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            complement(10438..10941)
                     /locus_tag="PC429_001459"
     CDS             complement(10438..10941)
                     /locus_tag="PC429_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF3846972.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            complement(10944..11447)
                     /locus_tag="PC429_001460"
     CDS             complement(10944..11447)
                     /locus_tag="PC429_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF3846973.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(11366..11662)
                     /locus_tag="PC429_001461"
     CDS             complement(11366..11662)
                     /locus_tag="PC429_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3846974.1"
                     /translation="MNDIFRDMQAKVGCENISDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            complement(11759..12361)
                     /locus_tag="PC429_001462"
     CDS             complement(11759..12361)
                     /locus_tag="PC429_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3846975.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(12446..12607)
                     /locus_tag="PC429_001463"
     CDS             complement(12446..12607)
                     /locus_tag="PC429_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846976.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            complement(12774..14684)
                     /gene="ltrA"
                     /locus_tag="PC429_001464"
     CDS             complement(12774..14684)
                     /gene="ltrA"
                     /locus_tag="PC429_001464"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDF3846977.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNHILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(15426..15560)
                     /locus_tag="PC429_001465"
     CDS             complement(15426..15560)
                     /locus_tag="PC429_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF3846978.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            complement(15568..16767)
                     /locus_tag="PC429_001466"
     CDS             complement(15568..16767)
                     /locus_tag="PC429_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF3846979.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPNNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            complement(16969..18363)
                     /locus_tag="PC429_001467"
     CDS             complement(16969..18363)
                     /locus_tag="PC429_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3846980.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFSSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(18480..18965)
                     /locus_tag="PC429_001468"
     CDS             complement(18480..18965)
                     /locus_tag="PC429_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846981.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            complement(19063..19446)
                     /locus_tag="PC429_001469"
     CDS             complement(19063..19446)
                     /locus_tag="PC429_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF3846982.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            complement(19461..19787)
                     /locus_tag="PC429_001470"
     CDS             complement(19461..19787)
                     /locus_tag="PC429_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF3846983.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            complement(20023..23067)
                     /locus_tag="PC429_001471"
     CDS             complement(20023..23067)
                     /locus_tag="PC429_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF3846984.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDNHNGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITNGNG
                     NYSIAGATYGVFSDKDCTKQIATLTTDDSGNTEVAEVKATTVYIKELSAPLGYKVDTT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAFLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGMELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPMKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            23595..24602
                     /gene="asrA"
                     /locus_tag="PC429_001472"
     CDS             23595..24602
                     /gene="asrA"
                     /locus_tag="PC429_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF3846985.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            24603..25394
                     /gene="asrB"
                     /locus_tag="PC429_001473"
     CDS             24603..25394
                     /gene="asrB"
                     /locus_tag="PC429_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF3846986.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            25407..26378
                     /gene="asrC"
                     /locus_tag="PC429_001474"
     CDS             25407..26378
                     /gene="asrC"
                     /locus_tag="PC429_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF3846987.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            26435..27211
                     /locus_tag="PC429_001475"
     CDS             26435..27211
                     /locus_tag="PC429_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF3846988.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            27482..29500
                     /locus_tag="PC429_001476"
     CDS             27482..29500
                     /locus_tag="PC429_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF3846989.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
BASE COUNT         8469 a         5765 c         5390 g         9916 t
ORIGIN      
        1 aatagtggga aatttttaag ctctgtgtcc gctctaattt tcaacctcgt tttattaccg
       61 caaactggac ataaaatcca tttatctttc aataatgcgt tcctcctatc taagtataca
      121 gttttgtaat ttatatatct ttagctttca tcttttccat ttcagtttgc tttatctttt
      181 tatatttatc cctgccagta ccccaatctc caaaaaataa agtattgact acaacaaacg
      241 ggaaaatata tggtctttca aatggtattc tgccacttgc aaaaacatat attgttcctg
      301 ccaagataag caagggaata atacctcccc aaagaggact tctaaatttt gtgcagagaa
      361 gatactctgc aattaataaa actcctgcaa caataaagcc aataattaat gtactcatgg
      421 ttatttctcc ttttcttttt tatatttttc aataattatc tttgcagttt ccacgtcaat
      481 tcgagaatca accgctaacc ctttaataaa agtttggaaa ggttcttctt cctgctggtc
      541 gcacatttca attttttgaa ttaatttgtc taaacgtaat cgaacagtag ggtaagatac
      601 cccatacatt ttcgctattt cctttaatga accggattta agtgcgaagt ttttaagaaa
      661 agtagcgtcc tcttgctcta aggcaagaat ccattgaggg attttatcaa gctccaaaaa
      721 cacaactcct ttcatattat ctttaacaat attcagtata tcataaataa aattaaagtc
      781 aataagaatt taaacatcaa aataattatt ttataatctt ccagttgctc ccgctttttt
      841 ccagcaccaa atcaaattgt gatacctgcg ttgcctttgt ctgctggtcg aggtacttca
      901 cagccacgga aaccgtcact tgactgtcga tgcggttgta gatgggattt accagttccg
      961 agaatacata atctttcccg ataggcttta acacgccctc atttacataa taggacagct
     1021 ctttagcggt tgcggttggg tagagcttaa agaacgtcgt cagaaattcg ttgatttcgt
     1081 ccgtcgtcac agcgtccacc gttccgtcgc tttcctttgc ctttggcgtg tagccggatt
     1141 tcacgggtat gctggtaatg gtcggattct tcgtgagaac catgtttcca gagccatcca
     1201 catagaccgt gacttcatag gcggagcgta ccgtcttcgt gcttccattc tccgtgatta
     1261 actgttccac agtgaagatc acggagaact ggttttcatc cgcctgcgtg acagaccata
     1321 tctgtacccc attgaccgcc gaagacactg gaatatcctt gcggatggta tcggtattaa
     1381 gtgcctgcaa ttcctttgtc agataaccgc tgattgcctg cgtccttgct tcaatagctt
     1441 ctttgctgtt attccatgtg taataggact tcgcaaagtt cttcacgaaa ttttcaatcc
     1501 cgttggtatc ggtaagtctt aactgtatgg tttctgtttc atgtacggtg tgcatatcaa
     1561 tagcggtaaa attcttatat accccaaaac tcacgcttgc gataagcact acccacaatg
     1621 caatcacaga ttttttatgt gtaccgactt tcacagtacg cactttcttt tctttgacat
     1681 tctctgtctg tttcttattc ttcttaaaca tattttctaa gtcctttctt ttattttact
     1741 cgtcctgcac cgattaagtg ttgttgccag taactattat ttaagtctgc atatcctatc
     1801 ggatttcctg catggtacat ctgattattt cccacatagt agagtaggaa agcaccgcct
     1861 ttccttattt ctagggtagg tttgtgcagt cctctccccg aaccgtgctt gcatttctca
     1921 atgcacacgg ctctccatct ttgctccgtt acaggttatt tcttttcgcc atgatgtata
     1981 gccatgtgac acttgtggca taccacgatt gttttccgtc ttttggctat cattatcttt
     2041 tcccattctg attttccttt aaggttttta accttgttta catgatgaat ttcgtatctg
     2101 tcactttccg tacacccaca tacttcacat atcttcgcct ttaatcggct ttctaatgtt
     2161 gttacgtcat gggaataatt tttagctgtt tgtggaacaa tatcagtaaa cttctttcct
     2221 ttgcagtcag cataattcgc aaaatacatt ttctttatta ctgttttagt ttcataagga
     2281 atagcccatt tgcctgtacc gtctttatac atagctctaa ttttagaaac tttggaacgg
     2341 tgctttcctg caagtgtttt caaacagcta tattccataa gataattgaa atagattagc
     2401 ttgttaaaat tgcttgccaa cccataataa ttacagatac ctcttaattc tgcattataa
     2461 atagtcacta tttccaaatc tgtcctatta agcagttgtg gtcggtgtat aggttctaat
     2521 ttcccattgt ctgacctctg ctttacaatt ccatttgaaa ataaaaattt ctctatcttg
     2581 tctttgaatg gaatactcaa ttccaccttg ttattcatag aacgggtttt gaattttcct
     2641 tttggtttaa tctgctggtc acggcgtact ctgacatcat atccgagaaa acgagcagga
     2701 gtattactgt gtgtaatcag agttttttct tgactaagtt ccatttttaa tgtggtactt
     2761 ataaattctg taagttcttg cttaatcttc tcacaatcct gtcgattgcc attcacagct
     2821 attaggaaat catcagcgta acgcacatat tttattttct tgtcgtcaca ttgcttcgct
     2881 ggtagtttaa gcatttgagc cgttgcagat ttatattctt caatcagctt ttctctttct
     2941 ataccgttgc tatttttaat tttgttgtgt aatctttggc gtagtcctac cagttttgta
     3001 tattctggtg tataacgtgc ctttgttgcc acatcaaact ctttctttat cttcattacg
     3061 tgacggtcta attcattaag gtagatattc gcaagaatag gcgaaatgat gccaccttga
     3121 gggcaaccgc tatatgtttt gtggtatttc caatcttcca tataacctgc ctttaagaat
     3181 gaacgaatga gtttcagaaa acgtgcatct ttaattttct gattgataat ttccacaagt
     3241 accgcatgat tgatgttatc aaagcaccct tttatgtcgc cctctataaa ccagcgtgct
     3301 cctgtaaatt ctttttttat ttgagcaaga gcagtatgac aacttcttgc tggtcggaaa
     3361 ccgtgagagt agtctgaaaa aataggctca tagattgctt ctaatatcat gcgtattgct
     3421 tcctgtatca gcttatcagt aaagacaggc agtcctaagg gacgcatttt gccatttgat
     3481 tttttgatgt atgtacgtct tactggtttt ggttgataca tttcattttt taaggcttca
     3541 atgatatttt caatatatat ttcgctaaaa ccgtccgctg tgtcgtctgt tacacccttt
     3601 gttcctgctc ctttgttgga gtagaggtgt tgatatgcaa catagtatat atctggacgg
     3661 agtaaatatc gatagagcct tgtaaatact tcatcatggt tcttttttga attttcctgt
     3721 aatttggtta aaatctccat tgttggtttc atttgaggtt ttcctcccta atcaatttct
     3781 aattttaatt cgctaagact gctttccttc gccatgtgaa cggttttccc gtatccgatt
     3841 ttacggcttt ccctgtcttt tcagggttct cagactacta tgaaagctcc gttaccttgc
     3901 cagattttca gcgtcatctc acttagcata gccttgtgat ttatcactac aagagcatta
     3961 cacatagcca ttgtaggcat tctggtttag gtaatctcca gttgacgaaa gtagtaggct
     4021 ggtgtgaatt gtcggatatg ctttcgttcc gttactactg gttctccagc gtaccgtatg
     4081 agttaattga taacccactg aaaggcattt attttcagcg ttactcatac cacaggtttc
     4141 agacaagttt cctgtgtcca cacgtaacag aggtttggaa ctcacattca acaaatctag
     4201 ttttatcctc atattcactt aaccttgcag ttcagtcgtg ctctattgtc tttgaacaac
     4261 tttctgcttt cctgccgtgc tattttcccg tacagttttc acctttcggt taggcaggtg
     4321 gtttcccgtg ttatcttacg gggtagtgcc ttttttttct gtcccccttt ctttaatcta
     4381 cttctaactt tcgtccctct ggacgcacta ttcccacatg ggtaacatac gaaccagcgt
     4441 tataggttga atggaaaaag accaactccc cagcttttgc ctgcgataaa gggatatgct
     4501 gggttgcgtc atattgcatt tgtgccgttc tcggtaaaga gataccagct tttccataac
     4561 accattgcgt cagtcccgaa cagtcaaagg aagtgttggg attgctaccg ccatacacat
     4621 atttccagcc ttgatatttc aacgcttcat tcattacctt ttgtgccagt tctcccgata
     4681 cctgcggaac agttaaatac tgattgacta attccacata aaacatattt ccatagccat
     4741 accgccagcc cccatttttc gcaacggcta tcgggttggt gtaggttact tttttaccgc
     4801 cagatttctc acgggcaaag ctctctgcaa ggttaaaagt atgtttcttt ccttttcctg
     4861 ccacatatcc cacatagcca ccgccatagt tataggactg tatcgccaca ttcaaatcat
     4921 ttattccttg atttttgcag gaagaaagca gggacgcaaa atatttacac ccctgcttga
     4981 ttgagctttc tgtatctaac gagttgggcg gtaaaccaag actttctgaa ctctgcatga
     5041 catcttttgc cgtaccgcca ctttctactt gaatgatagc cagtaacaca ttcacatact
     5101 cagaaatacc gtattcttta gcgtactttt ccaccatagg ctgatgtttc aagacttctg
     5161 cggataaatt catacccgta atgcccgaag aaaagttgct gttctcgtcg tcgctgtccg
     5221 cactcatcag taccccaaag aaaaggacta tggaaaagag gataggaaac agactgccaa
     5281 tgatagcgat atgtttcaat ttcatttttt cttctgtcct ttcttcgtta caaggttacg
     5341 gttcttttca gtatgttctg ttgtacgctt tgcttgttca actgtttgtg cctttgtggt
     5401 acgttctgct ttgtaattct ggtgtgtttc atgcgatacc actttttcta catttttact
     5461 atgtactgtc tgtgccgatt gtggaatttt tccgtcagat gtacccgaag ataagggacg
     5521 ttctttcaca ctatttgttt tgactggctc gcctgctttg gaagttgaag ttgtagcagg
     5581 acgtttgact tcctgtgttt tctcaacatt tgtttttgga atggctgaag ctgtgactgg
     5641 tcgctcatgg ggacgggtag ctcccgttgt cgctgatccg tccgtctttc gtgcctgcct
     5701 tgcttcttgt gctttttgaa gttccatacg cttgtcagcg atattcttct tatgctgttc
     5761 ctgcttttcc aagcgtcccg actgtctgga ttgttgttcc tgcaccatgc cacgcttgaa
     5821 gtccgataca cttgtttttg ctttttcctt tgcggaatgg acggcataag cggtctgtgt
     5881 cggtatatcc ttgaccgttt ctttcatagc gtttgccttg tctttgacct tattcttcgt
     5941 atctaagaca gcacccacct ttgaaccagc acgttgtccc atgctggaag atgtattccc
     6001 tcggttgtct tttgctgaat tattccttgt tgtccttgca ttagaaaccg tactgccagc
     6061 caccgcacca gcaacaccgc cagcggtaac agcaccagca agccgtcttt ccatacgcct
     6121 agccctatgt gccatataaa gatacggtct gcggaaaatt cttcgtccca tgctttgact
     6181 gtcgttagcg ttcaaagaga acatactcat taaatcgcct aacttcatgt agataccagc
     6241 gaaacacact atctgcaaga acgccaccat aaagaacggg tagtctgtgg aaatgttata
     6301 aaacatactg gaaatgctaa atgccaccgt tacaatgagc gtaattcctg cccttgtcat
     6361 tatggtatta aataccctca cgattgcctg ctttgccatg ctttcatagc tcggtatcat
     6421 ggatagtaaa aagctgatag gtaaaaacat ggcgaagata ataaaaagta tctggctgaa
     6481 caacatcata cccgtaagca agaatacaaa tatcgtgata cctaagttga agaacaagag
     6541 gaagaacacc atacctaaac ggtttacgac ttgcggtatc gtcagattgt tgttgtcgtt
     6601 actttcgatt tctgttttca cgacttcctc tctggttttg ccgtcctcgt tctctgggct
     6661 tgccgataca agagcttcta cacggtctgt cccgatttcc tctgcgttgc tgttcccaaa
     6721 ctgtaagagt agccacggct gttccacttg aatagagaat aggctgtccc ttatcaagtc
     6781 cacgctgtcc ttgccctcgc tgtcagagtt tgggagcatg atttttgttc ccaagtccaa
     6841 agaagcggta ctgatgtcgc ttgaaaattc atttatcttt ttgatgtagt cgggagcata
     6901 ggcaataaat gaagccgaca aaataaacac cacaacaaag ttgataacgg cgtgaagtgc
     6961 cttgctggtt tctcttttaa tcagtcccgt ataggcaaca tatagtccca ccactaagat
     7021 aatgagaagc aggaaaccga catagaagcc agaggttgaa aaaccgcttg aggtaacgcc
     7081 tgcaagggtc tgtatgctct gcccgatact gtctgccata tcattgatga agtccagctt
     7141 gtaggcttcc tgcactacat agcccgtggc attacttaaa tataaactga tagtccagac
     7201 aaaattagtg atacagtaca aaccatattg tactgatttt ccaatcccgt ccagccagtt
     7261 ccacggcaac caagcccagc tattatccac atagaagtca agctgatagt tggaaagcgg
     7321 atatttggaa tatagatttt ctgcgtttat cgtatcgtcc acaagccccg tcgcatgagc
     7381 caccgtcccc agaagtgaaa gcaggataag ggagagtgcc accacgaaca gagccatttt
     7441 gagaaagtgg aaaatcttct tttttgtgaa cgcactctgt atcctttctt tcatcactcc
     7501 acctcatttc tctgtatagg cggtctggta tcaaaggcgt gtagcagttc ttcaaagacg
     7561 gggtgtatct gtaccacgcc gacacgcccg tataagtcct gcaacaagca ttgtccgttc
     7621 tccaaatcac gaagtcgctt ctgattgttt tcgtcctctt tgtcgatacc gaaaaattct
     7681 aaagtctgtt ttatctcgtt gatgtcagta gaacggaaag cgaattttaa gccgatattg
     7741 tttttcagac tttcctttga cacgtcccca gaagattgcg taacgaaata cacacctgcc
     7801 tgcatagctc gtccagcacg caccagcttg ttagataaaa tttcgccttg tgccacattt
     7861 aagaacgccc acgcttcatc aaggtctacg attttgaaga tagagcggtc gctgtggata
     7921 aaatctaagg caaaggtact aatcacaatc agcatggaaa ccgatagtaa ttcaatggtc
     7981 gtgtattcct caaaagtcgt gtccttatct ggcagtacaa ggtcggctac ttgaatgata
     8041 ttgagctgat tatctaaact gatagcattt tccaccgttc cgtctgaaaa cagaagatgt
     8101 gcaaaatcat agtctgtaaa gctgtcgata tggtctgcga tattgcgtga aattgcggta
     8161 tcttcacagc gtaactcgtc tatcacatga agcaagcccc ttgtttcact ctgggtaaca
     8221 gaacgcaccg ccttacgaag tacggggaat ttttcgccgt ccctagagga aataccagtt
     8281 aaaaaggtta gtatgtcgat tgccagactt tcagcgtctt tcacattctt cataatcaca
     8341 aacgggtcaa gaagccctgc gttgtccttg tcgcttgtta ggtttacgat attgatttca
     8401 tgggcgattt ctggaagtgt ttctttccag tttccacgct ctgattttgg gtctaagata
     8461 accgcttgac cgccaaacaa aacagaatag tacacaagca ggttattaca gaatgacttc
     8521 ccaccgccaa gcgaaccaac aaaagcagaa gcaagggcat ttgtaactgt accttttacg
     8581 ccttgactgg caagggacgg ttgcagatat acatttcttc ccgtatcaac ggaatagccc
     8641 atatagatac ccgtgttttc ccctaactgc tgtgtcgcac caaaaccaag cccagctaag
     8701 aagtctgatt ttacatactg cacataatca ttgatatatc gcttgctggc tggtagaaat
     8761 tcagaatgaa gccccagcat atccccagca ggacgtacta acttcacatt caaatcgtca
     8821 taaaaatctt tgacttcatc acaacggcgt ttcagttcgt ccaaatcatc agcagacaca
     8881 cggataacat aagagagctt atacatactt tcttttgtct ggtctaaatc ggtttccagc
     8941 tcgtccacgc tgtctaaagc gtctactaca tttgagctgg tttcacttcc agcttgataa
     9001 gcgtgattat caaggtcttt cagttccttt ttcttgttgc ggacggtcgt taatgctttt
     9061 cggttttcca cgatttctac attcatgctc gtatcaacgg gaaaggtaaa ttgctgttgc
     9121 tggaaataga agatttcact agacggaaaa tcaagctcgc ctacaatcgc attgacggta
     9181 aaataggaca cataactttc cttgtcctca tgttccaatc gtaaatatcg ctggctttcc
     9241 tcaatcacac accttgtcgg gcggataagg tcgtaatact tgataagtgt ttctttctta
     9301 tactttttct ttggtagctg gtgctcataa tcttcatagg cgataccgtc cctgccgtaa
     9361 agatgttcca taagataccc aaagtcattg atttccagac ggcgaacctt aaagcgacgg
     9421 gagattttat tttccagtaa cttttccatt ttcatgtaac ggttgatttc atcattcggc
     9481 atggaaacaa agtcgttcat cagcgtgtgg ttcacttcat ggagaaactc tttgaacgtc
     9541 agccacgccg attttttaag gttcttcaga ttgagctgtt cttccgtaac catgagttta
     9601 aagccaagaa aaaagcggta gtccacttga ttgtccccaa tcattgatac taacgcttcg
     9661 gtctgttcgt ctatcttctg gtaagcgact tcctttaatt tccccgtaac caatcttttt
     9721 gactgctcct gcatacttct tatagagctt tccgtggcaa tctgcaaagc atgaatttta
     9781 ccctcacggg actgtgcgat tagctgtcgg aaactgtcat gcacgataaa tttctgttct
     9841 gcggataaga aagaatagtt atacggtatc agctcatagt aggcgaacac ctcattgtcc
     9901 ttgttccaga caaggttgtt gtcaatatat ttaatcggga acataactca cactccttac
     9961 tgccgtgatt gtttcgttga atatttcttt ttgcagtttt acggcttttc ctgcataagt
    10021 gattttcggt cgcaacgcat aggttatctg tgatttcaaa aagctgtatg gcttctttcc
    10081 gtcaaaggtt ttctgcgaca taaaccaagt gaggaaagca ggaataccga agtattttaa
    10141 aaatgctccc tcaatcatgg aaagtggggg catatcccca aacagaatga tgataaattc
    10201 tgtaatcaca aaccatgtaa tctgtgtaaa ggtaacggga aagggcaagt taaagtcatt
    10261 gattgcatac aagacttttt ctacattcca gatacccgtg tagcttctaa tctttttcaa
    10321 gttctttcaa ctcctctcat aagtttctaa atggaaaagg acagccctgt ttcagactgt
    10381 cccttaaaga gaaatacgcc gtcagtagca acaatgcttg tcgctgatct tccagcgtta
    10441 atatggtatc tcgcacattc ctcggcttgt tgcaatgaag caaccgccca tatccaaatc
    10501 tcgcccgtat gcttcatagt caatatagtt ctgcaagcta gggggaatat cgccaagtgc
    10561 ctgcaattca tctatgtagt aataggcaac atctgtcatg gtttcacagt cgggataata
    10621 gtaaatatcg tccttgtgtt ccacgacttc ctccagcgtc ccataatgac caataaattc
    10681 gtccaaacaa tccacgatat agtcgggaag ttcctctatc atttcataca tttcattgag
    10741 ttcatcaatg gaaacatact ccccaatctc aatcgggaag ttatcggtat catggattgc
    10801 gtattcttca tactgttcat tcaagccgat tttttctttc acatcttcct cgtcaatagg
    10861 gaaagtaaac caagcaccga ctaaatagcc ctcattatac ttgccaagat tagcgatata
    10921 aactgccata tcatcaatca tacttactct ggcaattcaa agataccgtg ttccgttact
    10981 aaaaatttcc cgtgttcctg caagtgtgaa gcataggttt caaagtcaaa atagtattct
    11041 tgacagtcct cggataaatg tttgaatgta gggtcattca tcagcttttg ccttgcaaca
    11101 tcaatcatgc ttttacagtc gggataaagc gtaattacat tcctgcaaat ataaagtgct
    11161 tctaaatttt catacaccgt atgaagcatt gtatattctt cctgcatatc gcttgaaagc
    11221 tggcgataca tataatctaa ttcattgagc tggtacacgc ttgtatgctc atggacttca
    11281 tcagcataag gcaacaattt ttcaataatg cggtaatctt gactttctgt actgacacct
    11341 aacttttctt caaactcggc aacctctatc ggcaggtcga accagtatga tgttgtttct
    11401 tcgccagttg ttcttttcgt ttccaccagt acccttgttt cctgcattgt tcctcacgtc
    11461 ctttctgttg tttcatcaca tcttttaaag tctggcatga cataccaaac acatattttg
    11521 aaaaatcttc taactgtttt tcttcatagt cggcaaggtt cagttgcttc atttcatgcc
    11581 acaccttacg cttgtaagag ggcaggtctg aaatgttttc acaaccaact tttgcctgca
    11641 tatctctaaa aatatcgttc attacatcac tccaatctga aatttaggta agaaaaaagc
    11701 agtcaatcct aagactaact gctttgtgtg tatggatatg aagttgtcaa actgggattt
    11761 atctggcaga cttgtttatc cactcatcaa gaaatttcat ttgttcttcc gtgtgaaacc
    11821 aatgctctcc gtttctcata acagtaagtg ttgcattagt ttgttttgta aattcagata
    11881 tagttgcata agaagtcaag ttatctttct caccaaataa aatatttgtt ggaattttcc
    11941 atgtagttgg attttctttt acataacaga gatattccca tgagagtgtt tctccaaaag
    12001 ttgttttaat ttctttttta tctcgaagtt catcttctgt aacatttgcc caatacatca
    12061 tatctgcaat cagtttttcc atattcacaa taggtgaaat aaaatatgct ttttctattt
    12121 gcttatctga taaggcgttc atagcgaaat aggcacctat gctatttgca actatttgaa
    12181 cagatttgta atgtttgcaa atcaaatcaa aaagtgatgg aaattcttct tttgcttccc
    12241 atggatattg tgcagtatag ttgagtccaa ccacatcaca atcactaaaa agaggtttgt
    12301 aatgtgaaga ctcctctgca tttccacctt taccatgtat ataaataact actttatcca
    12361 taatagttgt cctcctcaac ttcaaatttg tcgctttctt catatccaca ttatatcatt
    12421 ctcatatcag caccacaata aaattttacg caccgataat cttattgaac agctctaaca
    12481 gtacgtcttt cacaccgcca gcattgaata ccaatccaac ggcaacaagg gcaactacca
    12541 agaagccgat aagtttagaa aactcacgtt tgaaccctaa gtagatacca ataaccacaa
    12601 tcgccataag cactaagctc tgggcgttgc ttaaaaacca tgtgtatatg gataggtaag
    12661 ttcctgatat tataaatatc tcaaactttt ccccccattc acacggagcg tgcgactttc
    12721 aacgcactcc gcgttccatc gtgtttatat tgaaaagtat ttatgtagta gacctatatc
    12781 aagtttaatt cttgattgcc agctaatact ctatatttat ttagtcttat gagctggact
    12841 ttatttagct tcaatagagc tatatatttt tgtattgttt cttctgtttt agcgtgtatt
    12901 agacggtgta ccgcctcttt taccaaaata tgatttttat aatcgtcatt tccaccagct
    12961 ccagttggta caatatgatg acaatgtatt tcattagctt gttgaaattc ttccagagtt
    13021 atagcacatt ttccatgttg agcagaaaat agcgaaattc gattgtctgc gtattctatt
    13081 gtgcgatttt ccaaagataa tttcattaac tggtacatca gtaatttatc aacacttaga
    13141 ttggtgtgga taaattttct tccctctaga gtgtagcaat tcgctaaata cacctttgcc
    13201 attggttttc tgtgttggat atatccaaca gggaaaatag gttcgccctc gccagcaaca
    13261 taccggagca ttttagattt accatatctt gttgcctcct ttgtcgtaag ttgtctgcct
    13321 gtttttctca atctgcccct tttgtttgta cctaatctgt tagtgaagat agccattaca
    13381 gctctattca gtttcataca atccagattt acattcgttg caatctgata ataattttgc
    13441 atacctaata ccattgaatt ataaagtctt gtttcttcta attccgtttt cccttttgag
    13501 ggagaagcaa ttttctttgc ctgtgatacc agcttgtctt tttggttttt gaattgttta
    13561 tcacttatat gtgatttaac aactaacttc tttcttctcg gtatcatttt aatcttgaaa
    13621 ccaagaaagt cggagtatct ttttctaaca tttacaattc ttgttttttc ttgtgatact
    13681 tccaatttta atcgttgttc aatccattga gtgattgcta ttttggcttt ttcagcactt
    13741 tccttgtagc ggcagaaaac tctaaaatca tctgcataac gaaccatata catttccttt
    13801 aactttgttt tcttcatttc tttgtaagcg ttgcttttac atgggctacc gcttttattg
    13861 atatgaacca catagttttt tgtaacagga ttttcctgcc attggctttc tacccaatgg
    13921 tctaactcat taagaacaat gtttgctagt agtggggata ttattccccc ttgcggagtt
    13981 cctttctctg gatatgttat tgtaccgtta gtcattttga ttggagcttt taatatcctt
    14041 tttaaaatga atatcaagtg cttgtcccgt attcccatag cccatatctg ttttatcagc
    14101 ttggaatggt tcacgttgtc aaagaaaccc ttaatatcaa attcaatcac ataatgtagc
    14161 tggctgattt gtaggcggtt ataggttgct tttattgcat tttcaactga cctattaggt
    14221 ctgaaaccat agctgttttc actaaattta gcttcgcaga ttggttctaa tacttgtttt
    14281 atacattgtt gtataagcct gtcccacata caaggaatac ctagaggtct ggttttacca
    14341 tttggttttg gtatttcttt tcgcctcact ggcttaggtc gatacccatg tggactgcct
    14401 gcaacaatgt atcttacttt tttgacaact gtttctgctg gcaatttgcc aatatcttta
    14461 attattgtac cgtctgttcc tgctgtttta ctgcctgcat ttgctttgat atttcgatat
    14521 gccagtaata tattttctct cgataggata atatccataa gatgagtgaa aatatctcca
    14581 ttcttacttt tggcatataa atcatcaaag gtgttttgca tttcgtaata ttcagcgtgt
    14641 cttaaatctt ctacacacaa tgtttcattt ttatttttag gcataaggct tcactccttt
    14701 gcttgaaatt gagtgagctt ttttatctta ctcgtaatcc ttattgaaac aacataatac
    14761 ttttctaata tatacacgac ttgagggcat tcctccattc tcattacaag aatttcttag
    14821 gttcgccctc tgcttttcag cattagttca actgcttatt gttcttcgtc agtttattct
    14881 cttgatgaga acccaatacc tttcctcgtt ccgataatct tatctataca tatatatcct
    14941 taggtcacta gctataagcc tgctggcttg ataccgcctg taacgttata tggcatttca
    15001 taacctatat tcttactttg ccacaccagc tacgatttct cgtacctttg catttctaca
    15061 aagttcaaat ttagacccgt acattcgctg tttcgtcagt tccttataga acattctaac
    15121 catagatatt ttatagaccc ccgacctgtt ggatacgcta cccgcctctg gatagttttc
    15181 gatagaagat atatgttcat caactattac ggtatctttc agccgtcttt accgagcttc
    15241 aaacattctg agtttcctca tacaatgctt gtcggaggct cagggcaggc gtttcagagc
    15301 gttacttcat cattccacct gtcagattat cagttctcct aataaccaaa ctttttcgtt
    15361 cttttattag tgccttagct tttcactaag gaacgagtcg cacataaatt ttgtccgaaa
    15421 ttcatttaat tgtcctctct ttctttgatt tcttccatac atctgtcagc ttctttgccg
    15481 acagttaaaa tgcacatcaa gactacaccg atacccattc caaatgatac cagcaataag
    15541 tctttcaata attcccacat tttttcatca ctcctttacg ttggcaacca tttcttcaac
    15601 cgtggctgtc tgttgcttga ttatctggta gtgtcgttcg gtcagttttg cctgcttttc
    15661 aatcgtttcc agatactccg tctgattgtc cttgtctatc tgcttcaaca tcttcaaggt
    15721 cggtgcaacc tgcctttgta gccagttgag tgtacgctcc agcgtgtaag gttctggctg
    15781 tgttgtcagc tttacgggcg gtcgcccctc gccaatgaac caagcccagc gtacattggt
    15841 tttccagtcg ctttttcgct tgtctggttc tttatcagcg aagcggatat attgattgat
    15901 aatagaaaat gctgtcagtt ctgcgtcata atgtgtcagc agttccacaa cagcataata
    15961 ggcacgttca tttttcaaac gtatctcaaa tctatttttt atggggactt cctcaatcgg
    16021 tatgccaagt ttggcgtact gttcgtaatt cttttcataa cagcagaagt acacttcact
    16081 tgaaaatgaa ccaatataca gcgtgtgtcc cataccgtat ttatccgttt cattatgctt
    16141 taccagctct ccagaaccat agcttttgaa agaacggaat ttagaaatac attcctcatt
    16201 ctcgcatttt cgtatcagct ctggaatatc aagtaagccc gttcggtcat tgattgccaa
    16261 atcaagacgc ttcattacac cgccctcaac caatgcgtcc gtgaagaagt cataccaact
    16321 tcgctcttgt gccagcagat agctttcaaa ctggcgacag cctttgcctt tcagttccag
    16381 caggacacct ttttcttcgt cctgcgatac atacacaaac acatctccga tatggtaatg
    16441 ctcggtgtat ttgtaatgtc cgtaatcttc gtgaagcata tagccgatgt tgagcttcaa
    16501 tatatccttg atgatatgct ttatatccat tgtgggaaaa cggatacgca cataatcaaa
    16561 cagcaggaac atgggattgt tgggattgaa tttttccacc gcttctaaaa gcgtatgttt
    16621 catgtcctcg gtcagcgtta cctttcctgc ttcgatacgg ctcaaatgtt cacggctgat
    16681 atttgccatg attgcaagcc ttgtctgcga tacgccgtat gcacagcgtt tttctttcaa
    16741 ggcggttaaa aaatcagttt cattcagttg tcataccccc aatcgtgata attttgtgat
    16801 attgtgataa tttcaaggct ttatcacaaa aggcgaaatg tacgaaaaac ccttatttca
    16861 tgcggtcttg caaaggtttt tgtggaagtt tccgccgtaa ttgtgcccct ctgttagata
    16921 atgaggggct tgtatctgct ggcgtggcta acgccacacc agcaacggct agtccgtgcc
    16981 gtcgcctttc gcttcgcacg tcgccgtccc gtcctgcctt gcgtatgcaa gtgagcctat
    17041 ggtctgcaaa aaatcatgtc cttttggaac taagggtgtg taaaattcac ttatgacgct
    17101 tgttcccaca tcacaatagc cacgtccctt gatacgcttc tggaaaaact gttttttcac
    17161 atcagaactg aacagcatac cgtaacctaa ttcgctgata cgccccaagc caacacggaa
    17221 attaaaatta tctctgatac cgtccgagaa atattttgcg tctggacgct ggcaggcaac
    17281 aataaggaaa tatcctgctt gtcgtcctaa catgacgatt ttctttagct ggctaagtag
    17341 gctcacactt tcttttgtcc ccagcatttc aaaaaacgcc acatattcat caaagataag
    17401 aaagcagggc ggtaaaccaa gataggcata gttttcgccc gttttatagt ctgggtgtcg
    17461 cttcatttct tcgcttcttt gtaccatgcc ctcataaaag gcattgacac aatcaatcat
    17521 ttcttctttg gtgtgataca catttcccat taccgtacct aaatctgcaa ggtcagcgtt
    17581 cttcgggtct aatatgtaaa gaacagcgtt cgtatgtaac agagcttcaa taagcgtcag
    17641 cagaaaatac gttttaccgc caccagtccc accagcaatc agagcgtgag ggagtgcgtc
    17701 atattcccat acaagatttt tcatcagcct aagacagccg ttttctgctt tcacttcatc
    17761 aatggtaatg cggttcgcta tcatatcata aagcatggtg tattcgatat aaccgtcatg
    17821 taacgtcttg tcggtcagct cacaatacaa gccactttcc aatttatcct ctaaccgtaa
    17881 gagctggtct tgatattttc ctaatgtaat ttcacagcgg atatgaagca gtcctttttc
    17941 catttgataa taaatctttg gaaaccagat gattttttcc cttgatctgc tttgcaggtc
    18001 agtaaaaaaa ccgctgtctt gtacggtgtc tgcttcatac cacttgtttt ccagtatcat
    18061 tcgtgccagc ttttgacggt gcaggagctt cttgaaactg tcataacaga agcggtaata
    18121 cagaaaagcg accaatgcac aaacaccagt cgctatcagt agggttatga agttgtaagt
    18181 ggaaagcgtc aaaccgttgt ctaataagct gaaatgctcc caatcggtac gcatgagctg
    18241 tttcatattc agtagcagga taaccgcaat gaatacaaac agaagcgtcc ctatggaaaa
    18301 gtggtagaca aggttcttgt cgctggctct gatacggtgt cctttgttta aaatcatttt
    18361 cataaatcaa tcactccttt ctgggtacga aaaaagcagt caatcctaag actaactgct
    18421 ttgtgtgtat ggatatgaag ttgtcaaact gggatttatt gttcttaact tttctgtcgt
    18481 tataaagctg acttacgcac attaacctct gcacagacca gcccagctat atgctccgct
    18541 gtttcatttg tcagtacaaa gagcattccg tcattacggc attttttatg tgtgtcgcca
    18601 ttcaaggcat aaacaaaacc ttttacacag gtatcactac aggcattagg gtcaatcagt
    18661 ctcttgcagt cacgagaagt agtcattttc ttttgcatat cagcaggaag cgaagcgatt
    18721 atatcctcat actttccagc gttgtctccg taaatccgtg caaaaacacc tgtcttgcga
    18781 aacacccaat tcattactgt tttcttctct agttggtatg aagcggtgta accgcttttt
    18841 gcttccttta ttacaaggtc acagccctgc tcaataagtt tggtattaag tttctcgaca
    18901 aacgcttggt gttctggcgc gattgccgat agaaattctt tgaatgaacc tttttctttt
    18961 gccattatct ttttcctcca caaatttcaa tttatccgtt tcttcataca catattataa
    19021 cagttgctta tatccgctac aatgaaaatc tagtttgcca gcttatttct ttggttgccc
    19081 ctgcggtgca tggttctgtg gattgcctac ttgaccgcct ttattcttca agataatatc
    19141 gtcagccttg atataccagt ccacattgac acctcgatag ctggcgtttg ctaccgtatc
    19201 ggctacggga ttgacaagct ctacctctgc attatacgga tagtccttga cgggaatatc
    19261 agcaggaatt gatacttgta tcatacgttc ctgcacacta catttcaaat cataggttct
    19321 tcgtgtgatt tcttcacttg ttgaaccgtc gtcattttct gctcgcacct catgtctgag
    19381 ggcagagaac ttcaatacac cgaaagtttt ttccttgtct aatacaatac cgtttgctaa
    19441 tctcataatc atttaccccc ttacttgctt tcctcggctg gcaacatatc atcagccgtt
    19501 aagatatagt ttgtgaaccc tcgtcctttg accgctttac cgtctgcggt aatctttgga
    19561 ttgataagtt ttactcgctg ttctggtttg aaatgctttt cgcctgcttt cgctggaatg
    19621 gttacgataa tatcatcagc cctttgtacg tcagagtaca agttgtagct tcttgatacg
    19681 ggcgtaaagc gtccgttaat gcgttgctgt tcaatcttgt tttcgccagc aaattccaag
    19741 tttccaaaag tctgtgccat gttcggcact acatatttta attccatagt gttttacctc
    19801 gtttctttct tcttttttaa gtttgttgat tgtgctttct tattctggga attagggaaa
    19861 taccagtaat cccccagata gtaaagggta aatcaatgct tcgcaccctt gactatccgt
    19921 gtgcttgcag gttgttggca gacaagccag aataagtaaa cgtctgccgt ttgacagctt
    19981 cggcagagag cgtgattttt cttttctcat accgttaccg ccttatgatt tcttcatttt
    20041 acggcgtttg taaagatatg ttccagccag tccaccagca gatgtaagca acattaccag
    20101 aaatgccatg acatttgtgt tgtcgcccgt tttaggactg tctgttggtc tgctcggttt
    20161 ttctggtgtt gttggcgttt cgggtttctc tgggacttct ggtttttcct taaaagtaat
    20221 cgtctgtccc tcgtcctcaa tgtccttatg ctcagtaact tttttcggct cgtttggatt
    20281 gcttaaatca tacaattctt caaaagttac aagctgtttg ccgtcaagag aagtagcgtc
    20341 aaaggtaaag gtaacttcca ctttcatagt ttcgctgtca gcgataaacg tataatcact
    20401 ttccacacgt tttccattta taagaagctc tgcattttct tctttcaaca tctgccagcc
    20461 cacaagttta tactttgtac caacttccaa gccctctaag gttacagtat caatgattgt
    20521 tacgtctttt cctgcttcaa gctctttgtt accgtcctta tcggtagcag ttgtatgtat
    20581 tttgatgata cgttctgtaa taagtaccgt ctgcccgtcg tcctcaatat ctttatgctc
    20641 cgcaactttc atgggttcgt ctggattgct taagtcgtat aattcctcga atgttaccag
    20701 atttttaccg ccaagctcgg acgcattgaa tgtataagaa atttccactt tcatttcttc
    20761 atcatcagca acaaaggtat aatcgctttc tacacgtttc ccgtcaatga taatctcggc
    20821 gttttcttcc tttaacatct gccagccttt taactgatac ttagtgcctt ttgtaagtcc
    20881 gtctaatttg acagtatcaa cgattgttac ctcttttcct gcaaggattg tcttttcgcc
    20941 gtctttactg gttgctgtgg tatggatagt gatttccttt tcgtattcat cagtcaaagt
    21001 ccctaaatca atcacaagat tatttcttga taccacgatt tcaaatggtg ggataagttc
    21061 catgcctttg ttgctgtcag agcgtaattc ttcaataatg taagtatcgt aaggcaacgc
    21121 acctttgctg tcgtctggtt cagaagttcc aaaccataca ccgtcctcgc ttgttttgcc
    21181 agcgttggta ttgtgcttgt gcgaagccca ttttgaagaa gtggagaatt gcccattatc
    21241 gtcagttacc acaacatgac tttcgcccgt tgtcttgctt gtgatcctaa agggaacatc
    21301 agcaagacgc ttgtgtgtgc ctgcaccgat tttcacgccc tcaatatccc cacgcttaat
    21361 ctgattgtag atagagtggg cttcgtctgt taagtccacg atttttccgt tttctgtgat
    21421 tgtaaaatca atcggcttcg caccgtctgt caagtaaccg tttggagctt gtttctcaac
    21481 gatacggaaa ttcccgtaag gcaaaaggtc agcagaagta gaagcgatac cctcaatatc
    21541 cgtatggatt gtttttacaa cttcgttttt cttgtatatc ttgccctcaa ccaataccgc
    21601 attatcattt aaggaaatga tgtcaaaggc agtatctttc aaagtggcac ttccttgtgg
    21661 tttggtatct ttggtttcta aatctcgttt ctgaattttg acaccgccac gaatgacttt
    21721 atctgatacg gaaaactggt tacttccagt taatacggca aggtcgccgt cctcggtaat
    21781 ctgcgtaagg tataaggctt ttatctgttc ttcactacca ttcgcctgca tatatgcacc
    21841 gtctagtaag tagcccgttg gcgatttcgt ttcttcgacc gttagtgtac caagaggtaa
    21901 aaacgctttt ccgtcctgca tatagaagct atcgccagat accttgtatg cgtctgctaa
    21961 ttttgtgatg taatgggttg tcccgttact gtctgtttca gcggttgtct ttgtaaccca
    22021 tgtacgagta gcttcggcag gaagattgtc tttattgtag aagcctgcat aatatttcca
    22081 tgtaaactcc gccccctcta aagaagcgtt cccttgtggg ttggctttct gtgtttccat
    22141 gtcaatttta aaaagctcaa tcaaagtatc ggtaactttt ggcgtatcag atactttcaa
    22201 agtcgctgtc tttccagctt cgacttttaa ggaatacacc gtagtatcaa ctttataccc
    22261 caatggtgca gataattctt tgatatatac ggtagtagct ttgacttctg caacctctgt
    22321 atttccgctg tcgtcagtcg taagggtagc aatctgtttg gtgcaatcct tatcggaaaa
    22381 gacaccgtaa gtcgcaccag cgatagagta atttccgtta ccgttagtaa tgctggtatt
    22441 attggaagtc ttttgaagtt tggcatttcc gacatttaac ttcgcccaga attgccctaa
    22501 ttcctgcccc tcgccagagt agatataacc gccgcattca tagcgtcctt tgttttcttt
    22561 cacaaaggct tttgcaccag caaacacttc atcttgtgtt gcctttggta tttcatcata
    22621 agaagctcgc acgttatcac attgccagcc taagtgaaca ctcaatcttt gccacacaac
    22681 aagctgtctt aaaaggtagg cgtgattttt gcttatccca ttgtggttgt cggtgtattg
    22741 cttcacatat tcgatagata aggcaacatc acttatctgg tcggcactca tacgggagct
    22801 tgcgtcagct ctggtcttgt agccgttctt aaaatctgtg ttaatatcga tacaataagc
    22861 gtcctcgccc tcaacggtca aatgcccctc gttaaaggta gaaccaatcg aaccgtcatt
    22921 cattactttt tcaacgatac cgacacgttc ttcactttcc gtccagtatt gcttgctttc
    22981 tgcatggaca agtgtagtcg gtaaagcagt aacgacagtc gcaaaggcaa gtacgcccgt
    23041 acacaatcgt ttcaatatct ttttcataat tctaaatgct cctttcattt tgggtaataa
    23101 aatagccgtc cataatgaac ggctggaaat agaaaaggaa cgtcaattta ggcgttccat
    23161 agtctatgaa atattcaatt tttttcttgc ttcaatactg atgtgccgta ccatatcttt
    23221 gcatatctag gaaaacttgc gtatcaaggc tcattcattg gtagtaaatt agtagtaaat
    23281 tgatgtgttt gtttccttga aaatgctgat agatactatg ttttagcgga taatcaactt
    23341 tttagtatgt tttttgcata aaaattgagt agatagtagt tttattgtac ttaatgattg
    23401 tatttaagtg attagatttg aaattgaata atgtagataa taaaaaaata ttaacagaac
    23461 ttaataaact ccattaatat ttttttatta tttttgaaac tgtgataatt atcacggaag
    23521 catagaaaat aaagtgttaa gatttaaaca agatagtaag taagtaaaac taaaatataa
    23581 gaaaggtgat aaaaatgaaa ctaaaattag attcatctaa gttcaatgaa ggtctgaatt
    23641 atctaaaaaa agactacaag atatttgcac cagttttaat accatttaaa ggtacttttt
    23701 cagatactga tgttataaga tacaaggagg taaacgcttt tgaagaaatg gaatttgcta
    23761 agaaagcaaa cttttcacca aaagatgttg tacttcccat aaatcaagta ttgttttatt
    23821 ttactgaaaa agagtttaag gaaagcgatt tagacaacaa gaaaatttta attttcttaa
    23881 gagcttgtga cataaatggt ataaaaagac ttgatgaaat atatctaaat aatggtgaag
    23941 aaaaagatta tttttataaa aatatcagag ataaagtaaa gtttgcactt gtagggtgta
    24001 aggaaagttt tagaaactgt ttttgtgtga gcatggattc taacaaaact gataattatt
    24061 caattggatt aaatatagaa ggagatacac tgtacttaga tataaaggat agtgaatttg
    24121 aagtatttaa tggagaagca actgaattta atgttgatta tgtaactgat aatttaatat
    24181 ctgtagatgt gccagagaat attgattcaa atgaaatgat aggaaattca atatgggatg
    24241 aatatgatac aagatgtata ggatgtggta gatgtaattt tgtatgtcca acatgttcat
    24301 gttttacaat gcaggatatt ttctataagg aaaacgaaaa tgtaggtgag agaagaaggg
    24361 tttgggcttc ttgtcaagta gatggatata cagatatagc aggtggaaat tcttttagaa
    24421 aaaaacaagg cgaaagaatg agatttaaaa ctatgcataa aatacatgat tttaaaaaaa
    24481 gattcggata tcatatgtgt gttggctgtg gaagatgtga tgatgcctgt ccgcaataca
    24541 tatcattttc aaagtgtata gaaaaaataa atgatttagt aattagtaag gaggaagtat
    24601 aaatgaaccc atatatacca gtaagtgcaa aaatactaga tatagttaaa catactgata
    24661 aagaatggac ttttagagta aactgcaata caaaaggtgt attgcctgga aagttttatg
    24721 agatatctat acctaaatac ggtgaaagtc caatatcagt atcaggatat ggagaggatt
    24781 atattgactt tacaataaga aatgtaggaa aagtaactag cgagttgttt aattataaag
    24841 aaggagattc tttttttata agaggtcctt atggaaatgg atttgatgta tccctttatg
    24901 aaggaagaga aattgtagta gtggcaggag gaagtgcatt agcccctgta agaggaatag
    24961 ttgaatattt ttataataat aaagaaaaat gcgaaaagtt taaattaata gtaggattta
    25021 aatcaccaaa agatatttta tttgcagatg atttaaagag atggagtaaa aaacttgata
    25081 ttttagttac tgtagatgga gcagaggaag gatatagtgg taatgtagga ttagtaacaa
    25141 aatacatacc agaattggat atgaaagata taaacaatac ttctatagtt gtagttggac
    25201 ctccaatgat gatgaaattt acagtagaag aatttttaaa aagagattta gatgaaaaga
    25261 acatttgggt ttcttatgaa agaaaaatgt gttgtggaat aggtaaatgt ggacattgta
    25321 aaatggatga tacttacata tgtttagatg gaccagtatt tgattattca tatgctaaaa
    25381 atcttattga ttagggggtg aagtttgtga taagagattt aaataccaaa aaagtaatga
    25441 aaaatgctta tagaattaca aaaacaaaat atgagacttc tttaagagtt agagtacctg
    25501 gaggtcttat tgaccctgag agtttagctg tagtttctaa aattgcaagt gagtacggca
    25561 atggacaggt gcacataact acaagacagg ggtttgaaat attaggaata aatatggaag
    25621 atatggaaga aataaataaa attatacaac ctgttattga aaaaatgaat ataaatcaaa
    25681 gtgagaaaaa cgcgggttat ccagcagcag gtacaagaaa tatagcagct tgtataggaa
    25741 ataaagtctg tccaaaggct caatataata caactgaatt tgctaaaaga atagaaaaag
    25801 ctatatttcc aaatgattta cattttaaag tggctttaac tggttgtcca aatgattgta
    25861 taaaagctag aatgaatgac tttggaatta ttggtatggc tttaccaata tatgaaaagg
    25921 atagatgtgt aaattgtgga gcatgtgtaa aaaaatgttc taagatatct gttggagcat
    25981 taaaaacaga aaataataag gtggtaagag ataaagataa gtgtataggc tgtggagagt
    26041 gtgtactaaa ttgcccaaca aatgcatgga ctagagatga gaaaaaatac tatagattag
    26101 ctataatggg tagaacagga aagaaaaacc ctagactagc agaagatttt cttttatggg
    26161 tagatgagga cagcataata aagataatac ttaatactta tagatatgta gaaaagtata
    26221 tagataaaga tgcaccagga ggaaaagaac atataggata tataattgat agaacaggat
    26281 ttatggaatt taaaaaatgg gcacttgaag gtgtagagct tccagaaata actaaaatgt
    26341 acgaaaacat atactggagt ggaattaaat atctataaat tgagtaaaac atatatttga
    26401 ataaattaaa atttaataga aggaagaggg agttatgcat aaagaaactt tggataaatt
    26461 aactaatgca gctataaata agataaattt attaaacaca agtaaagtaa aatatttggt
    26521 atcttctgcg tttgcaggac tttatgtagg tataggtata cttttgatat ttactattgg
    26581 aggacttttg actgatgcag gttcacctat gactaagata gtaatggggt tatcatttgc
    26641 aatagcacta agtttggtta taatgacggg aacagagtta tttactggaa acaatatggt
    26701 tatgtctgct ggtatgttaa ataaaggtgt aagtataaaa gatacttcta aaatatgggc
    26761 atacagttgg gtaggtaatt taatcggagc tttggtttta ggcattatat ttgtaggaac
    26821 tggtctagta gacaaaggac ctgtagctga attttttgca aatacagcag ctagtaaagc
    26881 atccatgcca tttacagctc ttttctttag agggatttta tgtaatattc tagtatgtgt
    26941 atcagtgttg tgttcgttta gaactaatag tgatactgca aaaataatta tgatattctt
    27001 atgtttattt gcttttataa catcaggttt tgagcatagt gtagcaaata tgacaattta
    27061 tagtgtttca ttattttcac caacaataag tacagttact attggaggag ctatttataa
    27121 tttagtagct gtaacacttg gaaatattgt tggaggagct ttatttatgg gtcttggaac
    27181 ttatatatta ggtaaagaaa agcttaatta aatttattgt ataaattata actctcttga
    27241 taaatacatt tttagtattg ttgagagagt tatttttata tttttgaaaa aatatttgaa
    27301 acaaaaaaaa tatgggagca tacgaaaata tatcggttat atatataaaa aatacgttta
    27361 tatatgaatt atatagttct aaatataaaa aaagttaaag tcctaccgta aaaaagcgca
    27421 ttttgaataa gataacaaat atgataaaat tggtcttgtt gaaattttta ggggggatat
    27481 gatgacaaag aaactagtaa tatcaaaaac aaagttgttt atttttataa tagcatttat
    27541 ttctatattt atatatttat ttggaagtaa aaatacactt ataggtgtag gtatagtaac
    27601 agctatgcta acactcttgg aaagagattt aactatttct ccaattaaaa atttactgaa
    27661 gtatttagca ataaatataa tcttaggaat actatcattt tttgcagtac aaaatatgta
    27721 tttaggagtt ttattaaatt ttatagcatt atttataata gggtatgtat ttagttatga
    27781 cttaaaaaga gcagtatatg taccctttgg tctaatgtat atatttatgg taagtatacc
    27841 tgtaggaaaa agtgaatttc ctatgagatt gtcggcttta gctgttggag cagttataat
    27901 aatgattgct caatttgtta tgaatagaaa taggatgaaa aatgtcggag ataaagaact
    27961 gatatctata tgtgatgaat tattagaaaa aattagcctt ttaaaaaata ctattaatga
    28021 tgatagttct ataattaaat ctagtatgag aaaaatagat agctgtaatt atagaataaa
    28081 ttctatatct aaaaatttga aaatggttat ttttgataat agaaaagatg acttttatat
    28141 cagtattaga ggtatagaca taatgaatat tttattttca ttagaaagaa taagtttaat
    28201 cctggaaaga tacaaaaagg atagtaaaga atttgaagat gaagatataa aaaatatttt
    28261 agaagaaagt agcataaata gtaaatcaga tgttttagtt gtagcaacga aagaaataaa
    28321 ctatattaag atgtgtttag aaaataaaga taccataact gataaagaga tattaggatt
    28381 tagagattat gttattgctc aggatactaa aaatataaat ttaaaagaaa tttatagtgt
    28441 tttagaaaat ttatatgagt ttttagtaga ttataaaaaa gtaaaatcaa gagacgaaaa
    28501 gaaagctgaa agaaaatcaa agatacctca tgaatttaaa cgactatcaa tatataagaa
    28561 aaactttaat ttaaattcta taagattttc ttatgcagta aagattgctt tagctacagc
    28621 tgtagcgggt tttattatgg attatttcca tttaagagat ggaagatgga taatgttaac
    28681 tgtcttttca ttaacacaac cttacgcaga aaattgtata caaaggtcta gaaaaagaat
    28741 agaaggtaca tttataggag ctgtaatatt tattgtatta ttttcaataa taaaagatag
    28801 tacattaagg tcgttaatag ttcttttagc agggtatata aactcttatg tagttgatta
    28861 tagaaaatta atggtatgtg taactgtttc agctcttggt tcagcagtag ttatgggaga
    28921 cccaaatgtt ttaacaataa gcaggatttc atatgtagca ttaggagcaa ttattgcatt
    28981 aatagtaaat aagtttatat taccatatga tgctaaaaca ggctatcaac atgttataga
    29041 aatgtataaa ggaatagtta aaaatataat tgatgaagtc aataagtcta tagaaaatgt
    29101 agctgatgtt tactatataa aaaatttatt attgatacca tcacttattg aagatagact
    29161 tatgttgata aatgctatat ataaagataa acatcaagag gattttttag aaaatcagaa
    29221 attactaata agtaatatgt ataatctata tataaatgtt aataaaaata aaataaaaga
    29281 tgaagatgtt gaaaaaatat tgagagatac caattatatt tcaaattaca atgtagataa
    29341 gtatgatgaa ggaagaagtg taattttaga gagtattgta aatactaaaa gtctaggtga
    29401 caagataata tgtctaaact tactgcaaac tctaaatggt gtcaaagaaa tgtatagaat
    29461 aagtaatata actaaagttt caataaaaga agcagcttag atattaaatt atagaatagc
    29521 catatattta aaataaagta
//
LOCUS       DAMKMI010000035        29306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMKMI010000035 DAMKMI010000000
VERSION     DAMKMI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.036"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            383..3025
                     /gene="adhE"
                     /locus_tag="PC429_001477"
                     /gene_synonym="adhC"
     CDS             383..3025
                     /gene="adhE"
                     /locus_tag="PC429_001477"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF3846990.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3385..3726)
                     /locus_tag="PC429_001478"
     CDS             complement(3385..3726)
                     /locus_tag="PC429_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846991.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3753..4424)
                     /locus_tag="PC429_001479"
     CDS             complement(3753..4424)
                     /locus_tag="PC429_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3846992.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4436..7012)
                     /locus_tag="PC429_001480"
     CDS             complement(4436..7012)
                     /locus_tag="PC429_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3846993.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7220..8155
                     /locus_tag="PC429_001481"
     CDS             7220..8155
                     /locus_tag="PC429_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3846994.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8174..8911
                     /locus_tag="PC429_001482"
     CDS             8174..8911
                     /locus_tag="PC429_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3846995.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(9097..14154)
                     /locus_tag="PC429_001483"
     CDS             complement(9097..14154)
                     /locus_tag="PC429_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF3846996.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14179..14598)
                     /locus_tag="PC429_001484"
     CDS             complement(14179..14598)
                     /locus_tag="PC429_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846997.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14832..15083)
                     /locus_tag="PC429_001485"
     CDS             complement(14832..15083)
                     /locus_tag="PC429_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3846998.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15172..15903
                     /locus_tag="PC429_001486"
     CDS             15172..15903
                     /locus_tag="PC429_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF3846999.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15962..16873
                     /locus_tag="PC429_001487"
     CDS             15962..16873
                     /locus_tag="PC429_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3847000.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKFIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16924..17736
                     /locus_tag="PC429_001488"
     CDS             16924..17736
                     /locus_tag="PC429_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF3847001.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17845..18537
                     /locus_tag="PC429_001489"
     CDS             17845..18537
                     /locus_tag="PC429_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3847002.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18624..19565
                     /locus_tag="PC429_001490"
     CDS             18624..19565
                     /locus_tag="PC429_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847003.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLNQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19602..20759
                     /locus_tag="PC429_001491"
     CDS             19602..20759
                     /locus_tag="PC429_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF3847004.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20911..21471
                     /locus_tag="PC429_001492"
     CDS             20911..21471
                     /locus_tag="PC429_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF3847005.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21499..21990
                     /locus_tag="PC429_001493"
     CDS             21499..21990
                     /locus_tag="PC429_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847006.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22219..22770
                     /locus_tag="PC429_001494"
     CDS             22219..22770
                     /locus_tag="PC429_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847007.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22856..23788
                     /locus_tag="PC429_001495"
     CDS             22856..23788
                     /locus_tag="PC429_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF3847008.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23843..24706
                     /locus_tag="PC429_001496"
     CDS             23843..24706
                     /locus_tag="PC429_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF3847009.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24953..26815
                     /locus_tag="PC429_001497"
     CDS             24953..26815
                     /locus_tag="PC429_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3847010.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26818..28284
                     /locus_tag="PC429_001498"
     CDS             26818..28284
                     /locus_tag="PC429_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF3847011.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            28308..29144
                     /locus_tag="PC429_001499"
     CDS             28308..29144
                     /locus_tag="PC429_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3847012.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10861 a         3516 c         4678 g        10251 t
ORIGIN      
        1 tataaaaata cgacaaaaat tttatttact acttatacaa ataacatgca aataacatat
       61 aaaatttaat aagtaaaaaa aatgaaagat ataaattatc attttagtat tttatttagt
      121 cgaagtaaat tattcaaatg aaatatatct catttagata aaaaatttta gtaaaagtaa
      181 aaaaaataat atatttgtta aaaaataaat tgacaaaata ttcagaaaaa aacttcttga
      241 cctaagagag taaaataaat aaaatctaag gtatagagat gaaatctata aaattatttt
      301 aagtataagt taaaaatata gatgatgcat ttaagattaa taaaaattag agatgcataa
      361 ttattttagg gaggttttta gtatggagaa aaaagaaaag gtagtagaaa aatcaaatgt
      421 tgaagtatgt agtcctgaaa ttgtcaacag tgttgaaaca ttaagaatga gattagaaga
      481 aattagattg gcacaaaaag aatttgcaac atttacgcaa gaacaggtag ataagatttt
      541 cttagctgct tcaactgcag ctaatcaaca aagaatacct ctagcaaaaa tggcagtgga
      601 agagactggt atgggtattg ttgaagataa ggttataaaa aaccattttg cctcagaata
      661 tatctataat gcttataaag atactaaaac ttgtggtgtc atagaaaaag atgaagcatt
      721 tggttttaca agaattgctg agccagtagg tgttttatca gcagttatac caacaactaa
      781 tcctacatca actgcaatat tcaaatccct aatagcacta aaaactagaa atggtataat
      841 tttctctcct catccaagag ctaaaaactg tactatagaa gcagctagag ttgtacatga
      901 tgcagcagtt aaggcaggag caccaaaagg acttataggt tgggtagatg ttccttcgat
      961 tgaattaact aatgttgtca tggcagaggc agaccttata ttagctactg gtggacctgg
     1021 aatggttaaa tctgcatact cttcaggtaa accagctgtc ggagttgggc ctggaaatgt
     1081 acctgcaata atagatgaga gtgcagatat aaaaatggct gtaagttcaa tcttagtatc
     1141 aaaatcattc gataatggta tgatatgtgc ttccgagcaa gctgtaatag taccagaaaa
     1201 aatatatgaa gaagttaaaa aagaatttaa atatcgtgga gcacatttcc taaataaaga
     1261 agaaactgaa aaagtaggaa aagtagtaat aattgatggt tcattaaatg ccagaatagt
     1321 tggacaacca gcacatgtaa tagccaaaat ggcggatgta gaagttccaa aaacagctag
     1381 aataataatt ggtgaagtag aatctgttga attaaatgaa ccttttgcac atgaaaaact
     1441 ttctcctgta cttgcaatgt acaagtctaa aagttttgaa gatgcagttg ctaaagcaga
     1501 aaaattagtt gcagatggag gctatggtca tacatcttca ttatatgcag actctataaa
     1561 tcatccagag agagtagaaa agtttgtaaa tgctatgaag acttgtagag tattagttaa
     1621 tactccatca tcacaaggtg gtataggtga tttatataac tttaaattgg caccgtctct
     1681 tactcttggt tgtggttctt ggggtggaaa ctcagtatct gaaaacgttg gagtaaaaca
     1741 tttgcttaat atcaagacag tagctgaaag gagagagaat atgctttggt tcagagcacc
     1801 agaaaaggtt tatttcaaaa aaggatgttt aggtgtagca gcaagagaat ttaaggatgt
     1861 tatggataaa aagaaagcat ttatagtaac agattctttc ctatataata atggatatac
     1921 taagaaatta actgatttat tagatgagat gggaattaag catactacat tctttgatgt
     1981 agctccagac ccaacattag cttgtgcaag agaaggagct aaagctatgg ctgatttcca
     2041 accagattta ataatagctg taggtggagg aagtgcaatg gatgctggta agataatgtg
     2101 ggtaatgtat gagcatccag aagtagattt ccaagactta gctatgagat ttatggatat
     2161 aagaaaaaga gtatatgtat ttcctaagat gggtgaaaaa gcgtattttg ctgctatccc
     2221 aacttcagca ggaactggtt cagaagtaac tccatttgca gttataactg accaagattc
     2281 aggtgtaaag tacccattag cagattatga attaatgcca aacatggcta taattgatgc
     2341 tgatatgatg atggagatgc caccaagatt aacagcagca tcaggtgttg atgctttaac
     2401 acatgcatta gaagcatatg tatctatgtt aagaactgaa ccagctgacg gtctagcact
     2461 acaagcaggt aaaataatat ttgaatattt acctcgtgca tataaaaatg gaaagaatga
     2521 taaagaagca agagaaaaaa tggcaatggc atctactatg gcaggaatgt cttttgcaaa
     2581 tgccttctta ggaatatgtc actctttagc acataaatta ggtgcattcc accatgttca
     2641 acacggtgtt gcaaatgcat tattaataaa tgaagtaatc aagttcaatt gtgcagaagc
     2701 tccaaataaa atgggtgcat tctcacaata cagatatcct gattgcatac aaagatatgc
     2761 tgaatttgct tcttttgcag gtattaaagg aagtacagac caagaaaaag tagataatct
     2821 tataaaagca atagatgaac taaaggcaaa agtaggtctt cctaagacta taaaagaggc
     2881 tggagtagaa gaatctaaat tcttagaaag attggatgct atggttgaac aagcttttga
     2941 tgaccaatgt acaggtgcta atcctagata cccattgatg agtgaattaa aagagatata
     3001 tctaaaagtt tattatggta aataatatct attgtgcaac ataggttcta ttgggtttgt
     3061 atataaatta ttagatttga atataaatag agttttatta gagacacgta gaagtaacta
     3121 taaataatag attataactt aaattctaaa tggaaatgat aaaaagtcag taatttaatg
     3181 gtaatttcga atatgtgtat ttaatcaatt aactttaaaa ttagtcggaa aattttttta
     3241 ttacatataa ttttaaagca taatatataa aaaactttat gaaaaaatta taaaaatatt
     3301 cagaaaattc tattgactaa tttatttatc atgatataat aagtttagaa aagaaaaaaa
     3361 ttggggccta ttaaggcccc cttactattt tttgatagat ttatttaaaa ttactttctc
     3421 attttgattt tttatattta ttatagagtc agtataatcc acttcaaagt tggcaaaagc
     3481 agagaataaa tctatatctc ttaacaaatg aattgtcata cattgattgt tcttatttag
     3541 ccttagaatc aatgctctta caaatggaaa ttgtattcta gaattaaata agttagaacc
     3601 atcagtatct atatctaagc tacgataatt aatatctata aatatatcta catcatcatt
     3661 ttcttctctt attattctgt agtcaaaatc caagtttaaa tttaaattac ctaaaatcaa
     3721 taacatttaa atcctctttt ctataaatag ttttagagtg ttaatttaag ctctccatct
     3781 attatagata taattctatc tgcttttcta gcaatatttt cattatgtgt tattattata
     3841 agggtttggt tatatttttt tatagaactt tttagtaaat ccatgacttc ttctgtagtt
     3901 ttactgtcca aatttcctgt tggttcatct gctaggatta tacttggttt tgcagataaa
     3961 gcacgtgcaa tagcaactct ttgctgttga ccaccagata attggttggg aagatgattt
     4021 ttcctttcat taagacccaa taaatttata aggtcatcaa tatattcttt atctattttt
     4081 ttattgtcta actttagtgg taattctata ttttccttta catttaatac aggaattaag
     4141 ttatactgtt gaaagacaaa tcctaccttt tgtcttctat atcttgctaa ttctttatct
     4201 gaaagacaac atatatcttt accatctacc tttatagtac cattacttgg tctttcaaga
     4261 ccacctacta aatgtagaaa tgtacttttc ccacttccac ttggtccaat tatagcaaca
     4321 aattcgcctt tatttatttt taaatttgca ttctttagtg catgtacaga tgtttccttt
     4381 ttcccataaa tcttacctaa attaatagtt tccaatatag gcataattat ccctcctatt
     4441 ctgtaatgtt tatagcttca actatactaa ttttatttat ctttcttgat ggaatatatg
     4501 tagctagaat accaacaatt atatttgcta aaataactag gatatatatt ttgtaatcta
     4561 ttgaaaatcc caatccatat gatacaaagc cctgcatata atacatttta aattgtatta
     4621 taattcctga cacgatagta attatacttg agagtacacc atataatatt ccttcataaa
     4681 gtatcatatt tttaaagcct ctctcagaga taccaatagc tcttaacatt ccaaattcac
     4741 tagttcttga agttaagtta taacttatgt tgtttattat gttaagtaca ctgattatga
     4801 acattattgc tactatacca taactataaa tcatagttct agatgtcatc ttatcagaat
     4861 tttctttttc taccatcata tctactgttg tagttccagg aacttcactt ccaattttac
     4921 ctaaaaggtt atttattttt ttcttgtttg cattttcatt aaggtctgca taaactaagt
     4981 tatacttatc tacaccagtt aattttttta ggtattcgct actcgttatt acatctacac
     5041 catcatctcc agagtacata ccattatctg caaaaggata acttataata gctccaactt
     5101 taaattcata ttctaagtat tcataattat cttttagact ttcataaagc cctgtatcca
     5161 tcccaccctt tggatatttg acttttatag tatcacctac ttttatatct acagctggtg
     5221 ttccttcacc aggagtaaaa gtctttgtgt atttctgtgt tttagaaatt tctggtacat
     5281 atacaatagc taaattttct tttttcattt ttttcaagtt tatatttcca cttactaagt
     5341 atttttctaa tgactttatc atctcatcat tataaccttt tagcttttgc tttagtgtat
     5401 atttgccagt agtcttattt tttatgaaca gaggatttag cttataataa ggagttgaat
     5461 ttatagcttc gtagtaatct aatctattta ttttatttac ctctattctt gtgtttaaga
     5521 tactagcagt tttgactttg tcaattccat ctatattttt tattttatct atctgattat
     5581 cattaaaccc ttttatatct ttaaaagaag tattgttacc aatttcattg agataaacat
     5641 ttgatagaat aaaatctgca ttcatatatg tttgttcttg agtagatttg ttttgcacat
     5701 ctaaaaaaga atatttataa ttgtctttta tgacgattat acctcctata ctcatagata
     5761 atacaattat aatgaacccc tttttatttc taaagatgtt tttcgcagat atagattttg
     5821 ttactgacat atttaaagtt aattttgata ttaaataatt aactttggtt acatttttat
     5881 cagctccaaa agtttttctg atagcatcaa taggagaaat tcttcttatt tttaagtatg
     5941 taaagaaact tataattagt attattagta ttgtacatgc aattgataag agaattattt
     6001 tatctggaat aacaaaaggt gtaacattta tatttccttc aaattgaata tttcctacag
     6061 acctattaaa tatttgtgca cctcccatac ctatacatat acctataggc attgctatta
     6121 gagcaagtat gaaaagttca taaagcataa gtttgaaaat cctaaagttg gtggaaccaa
     6181 tagctcttaa gacgccatat tcctgtactc tttggtatat tgatataaca tagatactat
     6241 atattacaat accagcaaat agactcatag caattgcaac gtatttactt gaattatcta
     6301 aagtaccgtt tttcatagta gattctataa gcattgagtt tcttcctata cttttctcat
     6361 ctaacatagc attttttatt atattgtcta tgtttgtatt tatatcactg ccttcattaa
     6421 attcaatata agttgtaaaa tcattcagtt ctgattcatt gagattagaa aaaatttcac
     6481 attgtccctt ttttttctct ataaatctat cttcaaggat tccaacaact ttaaaagttt
     6541 ctggattttc tttttgatat aatttaaaag taaggttttg atttatctct ggttttaagc
     6601 ccatactatt taaaatccat ttctcgacta ccacctcatt gctagctttt ggaaatcgac
     6661 cttttataag tttagaatga ctagtcaaat aatttttatt tgcatatagt atgtttatag
     6721 gtaatttttc attaacatct gtagacgcat agtaagatgt tatacccaga tttttaatat
     6781 ctttgctaga ttttatggac tttaattgat ttttctttat atttttaaag tatacatggt
     6841 cagttccaag ctctcttttc aatctatcta catctgcttg ctgagcactt cttgagaggg
     6901 taccaatacc aacaacgagt gatgtagcaa gaattatact cacaacaatt gctaaagacc
     6961 tagctttata actttttata taccttagta ctattttcaa attatctttc atatttacca
     7021 cttcctttct taactaaatc aattatatat ttatctaaaa tatatagcaa agtaagtatt
     7081 ttcaagtttt aagtagttaa ttgtacaaat tcatctgtat ttttacaagt ttaattaatc
     7141 tatatgtaaa ttttaacttt ttacataatt tttttaaaat gatataatcc ctatataaat
     7201 tgtagagggg agggaaatca tggctaacaa ttttgtattt cctatagtaa tactaacttt
     7261 gtttatattt agcaatctta taatgaataa gtataaattt tacaaagaat tagacatatt
     7321 aaaaagaggc atattaacat tttttatatc gtgttttgca ttgattttat ttatggaggg
     7381 cactaaatat atacaattaa ggttaggata tagtttttta cttgaaggag tattaaatat
     7441 tatacttttt ttggttttaa ttattttaag tttttataca ggctttaaag ttataagatt
     7501 gataaataat aataatgaac aggaatataa actacaaaaa ctagatagta aaataaatca
     7561 taaggataat ataattatca aattaagaag tcaaaaacat gactatttga aacatttgca
     7621 agtggtgtat tctctattaa atactgggct agatgaagat gccaaagaat atataatgaa
     7681 tatatcaaat agttttaaat caaaaaattc aaggtatgga aaagtatctt atttagatgc
     7741 tatagcttca ttaaaatacg aggaatgtct tgaaaaggat atatcttttg atgtctacat
     7801 tcaagatttt atggataatt tagagattga acctaatgat atgagttcag tactattaaa
     7861 cataatagat aatgcaatag agtctttgtc tactataaaa aaagaaaaga agtatataag
     7921 agtacatgca tatgaagatg aatttcagta tattatagct ataaaaaata atggtcctaa
     7981 aatacataat gttgaaagta tattttggaa tggatttagt actaaagaag gacaagatag
     8041 gggatatggg ctacatttag ttaaaaatat attagataag tatgattata gtatacttgt
     8101 aaatagtgat gattttttaa ctgaatttgt aatcttaatc ccaaaatatt attagttagg
     8161 aggagaaaag tctatgataa aagttttgat ttgtgatgat gataagattg taagagatga
     8221 tatagcaaat atagctaaag agtctggata tgtagattat gttaaaaaag ttaaggatgg
     8281 tatagaggca attgaattta taaaaaatga gaaaatagat ttattgatta ttgacattga
     8341 tatgccatat aaaaatggga ttgattctgc tgaagaaatt ctttctatag ataaggatat
     8401 acatatagta tttgtgacgg gtttcgctga ttatagtctt gaaagcttta aagtgcatcc
     8461 tatagatttt gtagttaaac catttaaaaa agaaaaaata cttgattcaa ttaacatagc
     8521 aatagaccat attaactccc ataagattgc taataataat tttatagaag acaccctttt
     8581 tgtatataaa ataaggaagc agatacatat gattaatttt gatgatatag ttatgtttga
     8641 gaaaaattca agagcaatta atctacacac taaagatgaa gatataataa agttttatga
     8701 gaactttgat gatttaaaaa aacgcctacc tcctaatttc tttatgaccc atagacaata
     8761 catagtaaat ctaagattta tacataaggt tatacctata aataaatctt cattagagat
     8821 aaggtttata aattttcaag aaaatgccat attaaacaaa tctctagaaa aagatttttt
     8881 atatagattt tatagggtta aaagattcta gtgaagatag aagaaaaata ctttttgtgt
     8941 gatataataa gtaccataaa gataatgtat aagagaagtt tatataaaaa ttatttgtaa
     9001 ttatgattta atctatataa attacagtat agtatatatt ggactttcta atttttttta
     9061 agaaagtcca atatatttta gggatagttt ttatacttat aaattactta agatactagg
     9121 gtctttaatc ttactatctt cagcaagtaa tagtatctta gacataatct cagcagtttt
     9181 agggtcttca tcagcgaatg gaaggaatat cttacctcta ctttgagaat gtataggaag
     9241 tatattcaaa gcacccatcc cttttttatg tacaatacca gaacccatat gcacagaata
     9301 ctcacctaaa ttacctgcta taagagcatg agagccttca aaattgacat tgttaatttt
     9361 taataataat aaaagttctt tagcaattgc tatacgcatc tctacagtag aatggctagt
     9421 ttcagggtca acaccaccag catgagcgac acttacaaca aggtctatat cacgcataac
     9481 ttctgagaat agaattggag gtatagaagc taattctaaa cttttataag tttttctatc
     9541 aaaaaattct actgtttcaa tagtaggagg ctctatatct gctggtgaaa accaatctgc
     9601 aagtgcaaac atctttgcca ctatattttc tttataaaat actttctgta atccctcttc
     9661 ataatcgaca gtccaaagct gatttctaag aagtgctaca gttttctttg gctgaatttg
     9721 gtgaccagca tatctgcgag atatagtttt ttcagataat tcatcactgt ttattctata
     9781 atactcacgg aatacttgct taaatggttg aacaatttgc ttactaaata aatcatgttg
     9841 gaactcactc cacttaccag attcaaacaa atgaactgga tgtgcaagtt ttaatttatt
     9901 ttttgccgtc aacttataag tttcaccttc tatattgcaa ataaagaact tagacttatc
     9961 attataaaca tatccaatat tatcatcaca tacccatact agatttttaa tcaaagggca
    10021 gataactgga tttttataga tattggcaat ttctttaact gtaaatacta tttcattttt
    10081 catagcttgt tctaaactct gttttgcacg agaatactga ctctttaaat ctttttggac
    10141 agatttaagt tcaagtacat atggatgttt agcaatagct tttggtatac tctttaaaga
    10201 cttgttgcct ttttgaactt tagtagatgc ttttccgttt tcatcaacat ctatccaaat
    10261 tgaagtacca tcaatatctt ttggtttaaa tagatgagca acttcattta gtttttcagt
    10321 ctcaagagcc cacatcatac gatttacatc tccaaatcca gcacttctag ctagattttc
    10381 tagcgctata gtacaagctt ttgattcact ttcacgacgt tgtgcaccaa attgcttgct
    10441 ttctttagca aacttttgaa taaattcata acggaaagtt gcttctttta ggtctccttt
    10501 tttcatagga agtaaagaga aacttctcaa tttatcttga tttctatttg tttcaatttc
    10561 tttcatcaac atgtctttat caagcttacc aagtgtagca tcagcataga gttgtgcacg
    10621 tctatgagag ttaccaccac tagttatata tttagctgac ttgtaaagta tattgaatct
    10681 ttcttcccca atagttttat atgaatctaa aaaccaatct acatcaaatg caccatcaat
    10741 gaaactacgt ggtgaaatag gggagaatag agcaacctct gattcttttt gtgcagagaa
    10801 gccttcattt atatgtgcat ggaagtacca aattgtactt ttcagaccct tccagtttaa
    10861 atatttttca attatgttta cccattgagg ggcatacata gcagattcta tcaatcttaa
    10921 ctcagtaatg tcggtatttt taagtaaact ttttaatttt tttgagtcat cttctttagt
    10981 aggatagcaa tttttaagta gagaacttaa aacatctttt tttgaataat tgctaccata
    11041 tgaatatcct ctctggaacg tttctttacc aagagcgacc aaaatattta caaaataatt
    11101 tataccttca aagtattgta tattattagc tagatgagac acctctgttg gcatatcacc
    11161 tctacgtgat tcaatttcta taattcggtt tgtaacagtt tttaaattat ctttaagcca
    11221 agggtacttt tctaatgtgt cgccagccca tctaggattt gtcaatgttc ttattatatt
    11281 tgatgcgttg tatccactta tcatttctct attaacttgc tcttcagtca ttaatcctaa
    11341 ttcatgtgca cgagcaaaca catttatgct agtatagaaa ggaaacttat attctagcaa
    11401 ctgataattt gaaaacgtag tgtagaaaaa cttagtaaaa gaaatatcat cataaataaa
    11461 cgaatgtgct ttatcaagcc aatagtttaa aataggggca tttaaagcag ggcagtattc
    11521 attttgtctg taatagtttt tagtaaattc aactctttgt tttttacatt gttctatagt
    11581 caataagttt ctaagtttat agtatatttc taaacaatag ttaaatattt ctttatggtc
    11641 aaactcattt agtaaaactc ttaaaagttt tcttgtaagt ccattaaatt taagtttttc
    11701 aagttttgta tatattttat tttgtttttt agagtcaagg acatttgaat atatagatga
    11761 aaaccaactt ttttctgttt cattatatcc acatgtactt atactataaa ataaaatatc
    11821 aagtattaat ttgtaatcta agtcaagctt tttactagtt tctctccaaa gctctgctaa
    11881 tggataatta tcaagagtat cattagaaac tttatattta ataggagtca agttataagt
    11941 agaataacct aaaagtactt tgtctacatt acctgaccat ctttcaactt catattcata
    12001 atctgcattt tcagtaataa gctcatcaaa tttttcataa acttttatta ttttatttat
    12061 atccgtatcg agaatttttt ttgcttcttt ttttgttgct acagaaacag aatcctcacg
    12121 agtagataaa cttatatttg agataatttt attaagcttt gatgagctat tagaatcatc
    12181 tttaaaatca aattgttctg gaatttgaac tttgttttca gggttatata ttccaaaacc
    12241 attttctttg cagtaatcat tttttgcttt acctaacaaa ttatcaatta gtatttgaac
    12301 ttgagatgta ttctttttag atttatcaat atcttctata agtaaaacaa gcttattata
    12361 tagttcttgt ctagcagtat tgtcttttaa taagtttaca atttcaagac ctccaaggca
    12421 tttttgagaa tttgaagatt ttatcagtga agaagcaata ttttccaaat ttgaatcatc
    12481 ttgttcaagt aaaattttta ttactgtttg tctaatagag cttgtcttta aagacaatag
    12541 attggttatc ttatctaatt ctacatctgt tagctcaagc tttctaagta gactcaatac
    12601 cgtttctcta ttggaaacac tcttatcact taaacattcc aataaaaatt cacgttgtct
    12661 atctgtcttt ggttcattta caaaattgac tataagtgaa gctcttaaat ctgaactcat
    12721 aagatgacaa ttttccataa gcacatctat aaattgagca ttcatatcgt atgctgttaa
    12781 agtcatcata catttaagta tttcatgaga agaaatttca atccaagtcc atggaaatac
    12841 acttcctgaa aatgattttg tctttgaatt taaaatttct aaagtatgtt ttaaactttc
    12901 aaattgttct tttctagcat caaagtcttt gtcaatagga gtgtttgaca agtcaaaatt
    12961 agtttctctg ttttgccaac tatagtagta gccaacagaa ccttctacat agaaatttgt
    13021 aattacccat gctagtatct ctaaatcatc acccttaatg tttttagatg ctatagaatg
    13081 tttaaaatat gtactttctg tatgcgataa aaagtaaaga gctacaagtt ttttatattt
    13141 ttcctgacaa gaaagtaagt tttcaataag agagtaagag tcatatatgt tgtaagttgc
    13201 agtagcccat aaggcaaaat aaatttcaag attatcattg ctattaattg attctatacg
    13261 gaacttttca tcagtcaatg atttatatga aagttctaaa catttacgta ttattccagg
    13321 tttttggtct gaaaaaccaa gtcctgtcca tgtatccaag gctcttataa cagaactaaa
    13381 tcgctctaaa ttgttgtcca aaatagtttt taacatgtat attgatgcag aaattgtacc
    13441 ctcatctaaa ctctctacaa tttgctgtct aagtccttct tgttgtttgg ctgccaaaag
    13501 aagtttagaa agcatttcat agcatttatc actatgactc ttcatgatac caattatcat
    13561 ttgtcttgta agtagagcag tttgattttc tccaaatatt acttcttcta aagcacttat
    13621 tactttttga tttcctttgt ctatttctag tgctatttta tcactcatta tagagtcata
    13681 gtacaagacc ttatttggaa gagtaagata atctataact gaaaaatcat aataaattaa
    13741 atcaacatag tttgaaataa accttataat acctctaaaa taatccacaa gtttatttga
    13801 gcgataccct ttacgataat atcctcctga atatgtataa tccatctttc ttttaattaa
    13861 ttggcaaagt tcttctactt gttcagaagt aaatattaat tctaatgcat tttttaaagg
    13921 tccagaaagg agctcgtcca tatttttaat ttcgtagtag tggcaattgt agtatttctt
    13981 tatcatattt aagaatttgt cttcaggttc tctataatag tatgaactat tttctatgca
    14041 gtcccaaaga gactttgcta agtcgtagta ctgttttgaa gaagatttag taaaattatt
    14101 tttaaaattt tcaattattt taggattttt ttctactcta gtacgttcta acatttatgt
    14161 acctccaaat tatttatttt aaaacattct tccagcaagc aaggtaagtc ttataaaagt
    14221 aaatatatct ccacttttca gatttataga ggaatcgagt ttttcaataa catcttcatt
    14281 tataagcact ttgtagattc catcttcaaa tgcctgtaac gctacctcta ttgccttttc
    14341 tacagattgt ttattctcat tgtaaataga gccaaatcca acttttccaa tatcagcttg
    14401 agattcaatt tcttcattag ttaagaagtt tattattgat ttttctggga ttttctcatt
    14461 atattcaaga acatttttag atacaatttc agagatagct tcttttaggc aattgatgtt
    14521 gtcagataca tcaaaagggg taagttctag cataggtttg cgtttgccaa cagccttaac
    14581 tctaaaataa atttgcataa aatcacctcg caaaatatta attatgtatt tagaaattta
    14641 aaaatttatc tatttgttat attatatcat tttgtgaatt attaatgtga attatggaat
    14701 tattttacaa aaaatagaaa aaaagctatc ttaaagtcta aaaaagcaat aaaagataac
    14761 ttttaactat aattatatag aattataact gtacatttat taaattttaa ttttagaatt
    14821 atttttgata actatctttt tttatttata aattttgctc ctaaatattt tttgatatct
    14881 tcttcaggag aaaagtcatc aataagtatt tctttcccag tgtcttcaaa tactacaaga
    14941 acttcacagt caccttgtaa gaaaaatttt ctaactgtat cttctttaac ttctctaact
    15001 tttattacat tctttttttc tttttcaaag tcttttgtgt aataacttcc catcatatta
    15061 attatttttg tatatctatt cataaaatac ctccatatgc gattaaattt aaaaaatatg
    15121 ttataataat tgtttgtatt aacttaataa ttataacaag gagtaataat aatgactagt
    15181 ataaaaaata aagatatttt aaattgtata gattattcca ttaaaaataa gctctttgac
    15241 aaactaaatg atgtatataa gtccttgcca gtaggaaatt gttctggatg tggaaattgt
    15301 tgtatggagt cagtaggtat aaatttaatt gagtttttga atatattttg ttatcttgaa
    15361 gatagagttg atttaaaaaa aagctgtata agtaaaatat tagattatta ttttgaggag
    15421 tatacaagaa agaacccatg tccatttaaa gatactaata atagatgttt aatatatgaa
    15481 gttagaccat taaattgtag gatgtttgga cattggaaaa aagaggatta taataaaaat
    15541 ttagataatg ttattgagaa aaataataac tatagagagt taatgaaaaa gcaatatgga
    15601 tttgaaataa atgatgaagt agtaaattac aggatagatt attgtgatag atttattcca
    15661 aataaagatt atttaagtaa atctaagaga ttaagctttt ttgatgagct tatgatatta
    15721 gactcaaata tttattcaag tggaaatata gatatagact ttagggatag aggtatagtt
    15781 gaatatttta tagagtcatt actttataga aatttagcat ataatgtgaa aataaggata
    15841 tctaaagaat acaaaataag aaaaagaact atagatagaa taaaaaggat tgttcttgta
    15901 tagttttata ttagtaagta ataaattgta tcataaatat tgtcaaaatg aggtgaagaa
    15961 ggtgaatctt acaaaagtaa aaggaaatac cttttttata aaaggtggca cgaatacagg
    16021 aatatatgta tttgaagata acacagcact tatgatagac ccagggttat gtggttcaag
    16081 acctaggaga gtaaataaag tattagatga aaataatata caggttagat atattataaa
    16141 tactcatggt catgatgacc actatggagc ttgtaatcaa tttaaagaat attataaaga
    16201 tgtgtgtata atgtcatctg aatacgataa attatatata gaaaatccag aattgttttc
    16261 aaagtatatt gttgggggaa agagcaatgt atttatggat aatatcttaa aaaataaaat
    16321 gttggatgat ataaaaatag ataaatttat agaagctgga aaatttgaat taaacaatga
    16381 aatgtttgat ataattgaat ttcagggtca tacacctggt agtatagctg ttttgacgaa
    16441 ggataatgtg atttttgttg gcgatttatt gataggagat gaaattttat caaaatacga
    16501 tttcttgttt ctatatgata tacaagaaca aataaattca ttagaaaaat taaaaaatat
    16561 agattttgag tacttagttt tagggcatgg caagcaagtt atatcgaaag aagattctta
    16621 taaattaata gaaaaacatt tgaatgcttt aaaaaaatat ttatatcaaa taagagagtt
    16681 gcttgtagaa ccgaaatctt tagaaatttt attaaaaaac attataaata acaataattt
    16741 aagcaataat tataaagagt atcatttttt taaatcttcg ttagtatctg tcataagtta
    16801 cttagttgat ttagaagaaa tagggtatat attggaaaat ggagaattgc tttactatac
    16861 aaaaacaaaa taaattatga taaaataaag aaggtcataa aaaacaaata aagggtgagc
    16921 aatatggaat acgaaaaatg gaatgagata ctagcacctt atgaacatgc agtagaggaa
    16981 ttaaaaataa aatttaaaaa tataagaaaa gaatatttag caaaaggtga atattctccc
    17041 atagagtttg tcacaggtag aacaaaaaaa atatcttcca taatatctaa gttaaaaagg
    17101 ataaatgcaa aagatataga aactgaaatt gatgatattg ctggtataag aataatgtgc
    17161 cagtttgttg aggatatata tgctatagtt gatttgataa aagtaagaaa tgatatgact
    17221 ataataggag aaaaagatta tattacaaat tacaaagata gtggttatag aagctatcat
    17281 gttataataa aataccctat aaactccatt gcgggttcaa aggaaattat atgtgaaata
    17341 caaataagaa ctcttgcaat gaatttctgg gctacaatag aacattcatt aaaatataaa
    17401 tatgaccatt acatacctga gactcttgct gaaaggctta gaagagcatc tgatgctgcc
    17461 tttcttttag accaagaaat gagtgaaata agagaggaca taatgaaagc tcaggctatg
    17521 taccaaatga agtctatagc tattagagat actctaaata gaatacaaga gttatatgac
    17581 ttgggtgata cacataaagc tatgcagtac caaagaaaac ttgatagaac tgaaacagat
    17641 aaaaatataa ctgaaatttt ggaattaaaa caagaaatag atatattatt agagcaatat
    17701 aaggatacta aggtagatga taaagtagtt cagtaaaaac tacttgtaga aaatttgcta
    17761 aatattaatg gggctgcctc ttaatggaaa ataatttcta aaaagagaca gcctatttag
    17821 tgtaattaag aaaggaatga tattatgagt ttgtatgcta taggtgattt acatttttca
    17881 acatcagtca ataagccaat gaatatcttt ggaagtaatt gggatggtca tgaaaagaaa
    17941 attatagata attggaaaga agttgttaag gaagaagata tggtcttagt actaggagat
    18001 acatcttggg gaattaattt atccgaagct aaaaaagatt tagatataat aagcaaactt
    18061 ccaggtcaga aaatacttat aaagggaaat catgattact ggtggacaac cgttacaagt
    18121 ttaaataagc tatatgaaga catgcgtttt attcagacaa atttttatga atataaagat
    18181 tatgcaattt gtggaggtag gggctggata tgtcctaatg atgttaaatt tgatgagact
    18241 gatgaaaaag tttataaaag agaagaacat agacttagac tatcattaga atctgctcgt
    18301 aaaagtggtc actcaaaaat aattgtgatt actcattatc ctcctacgaa tgataaattg
    18361 gaagaatctt tatttacaaa gttatttgaa gaatataatg ttgaaaaggt gatttatggt
    18421 cacttgcatg gtaaagaatc ttttaaaatg ggattgaagg ggattcgaaa tggagtagaa
    18481 tatacattag catcttgtga ctatacagaa tttaatttaa taaaagttca cgattaaaaa
    18541 acataaattg gtaaatacta taagatacat gattaaaaat aaatatttga gggatttcgg
    18601 ttttgagaaa ggagacacga cttatgaggt ggatattagt ttttatattt ttatatctta
    18661 ttcctttagt agtgatattc agaaattaca agaattttaa gaggtcttgt atctacagta
    18721 gtatttatgt ggtattagct acaacaataa tgataagtaa tatttatatg agtggactta
    18781 ataaaataaa agagtctatg tattatcaca attatatttc tgatgagaca tatgatgaaa
    18841 agtatgtatc aaattttaag gaaaaagaaa gtactgattt aaaccaggat aataaaaaga
    18901 atgaagaaat atcaaataat aaaaatttaa acaacaaaca agatagaaat atacaaaata
    18961 aagaaagtaa tgaagaagga agtaatgata taaaagaaaa gaataattct gatagtaatg
    19021 tttcagtgaa taataaatca gaaaaacaag aagataatac aaaagaaaat aataaatata
    19081 caagtaaaga aaaaaatagt gaagatataa aaaaaataga tatggaaagt ataggaattt
    19141 ttaagaaaga aatttataat atagaaagag cagctttagt tccaatgaga gaatgcatac
    19201 cttacactaa ggatatagca aaaaatataa ctaaattatc tagtataaaa aaagatgtta
    19261 caaatgccag aaacaagtgt aaagaagttg taaaagaata tgaaaatatg cagattccag
    19321 aactttctaa ggagaagtat acagaaacat tgagtaatgc tagagatgac gtaaagaaag
    19381 catatgagct tagagaaaaa tctatggaaa gtgctttgaa attaataaat actaaaagcc
    19441 ctaaatatat agggaagata acagaatatc ttgatttatc agataagcag atagaaagtt
    19501 ttaaaaatag attgaacgat ttaaaagaag aaataaataa ttcagaaaca aataatatta
    19561 attaataaga tttaaggata taagttaaaa gggagattat gatgaataaa cagagtatag
    19621 gaaaactaaa tatgtttttt atggggatta ctcaaagaaa ccttgataat agggattttt
    19681 ttattgagtt agaggtgatt tttaaatctg gaagaaaaga gtttaaagga attgcaatag
    19741 aggaagataa taaatataaa tttaatttta aaggaaaatc agatttatat acgtttaatg
    19801 agttgcttaa gaatatttct aaagaggcag aaaattatga tggattagta tttaaatatg
    19861 ttgagcgagg aactattgtt gtaattgaag gagacaataa gaaggtaaat gtaaaatatt
    19921 tagataataa ggaagaagta cctaaaatag atgaatttac ggcatctcaa ataaaaaaca
    19981 gagattatta tgtaaaagta ggacaagcaa atgcactttt aaaagaaatt ggagtactta
    20041 caaaagatgg taagataaaa aatgataaaa taagaaaata caatcagata gaccattttg
    20101 ttgaattgat agatagtatt ttaaaagaaa taaaggataa agactgtata actatcttgg
    20161 attgtgcttg tggaaaatcc tacttaagct ttgtcttaaa tttttatata aaagaagtat
    20221 tgaagaaaaa ctgttatttt ataggaatag attattcaga tgtagttata gaagcatcaa
    20281 aaaatatggc taaaaacttg ggttataaaa atatgtcatt tataaaagag gatttgacta
    20341 attatacacc taatagggat gttgaccttg taataagtct acatgcttgt gatacggcta
    20401 cagatatggc aataggtcta ggaattagag caaaatctga agctatagta gtagtaccat
    20461 gttgtcataa ggagctatta gggcaatata gatatgaagc tatggaacca atattaaaac
    20521 atggtgtatt taaagctaga tttgcagatt taataactga tggtttaaga acattgttat
    20581 tggagggcaa tggatatgat acttctgtag tagaatacat atctccactt gatacaccaa
    20641 aaaatttaat gataagagcg ataaaaacta aaacgaataa tgacaaagca ttaaaagaat
    20701 ataaagaact gaaatcacaa tttggagtgg aaccaacctt agaaaaactt atatattagt
    20761 taaaaatgta aaatttacga aaagcttgct taaatatgca agctttttgg tattatatag
    20821 gtatagtcat atccagagtt ttgatttata attaaagctg tgctataata tttgattaat
    20881 caaatcaata aatgttacat ggaggtaaaa atgaacaaat tgaatatatg tattgatata
    20941 gatggaacaa taacaagccc ttatcatttt cttccatacc taaatgatat ctttaataaa
    21001 aatataactg aagcagagtg tataacacat aattgggaag aattgtatgg ttcgggaata
    21061 agagatgtgt atgcggagtt taataataag tatatacact catatgatga agcaaagatt
    21121 gtagagggtg ctacagaggt aattagcaca ttatctaaag gacataattt atcttttgta
    21181 acggctagac atgagtgttt aactgatgta actaaaaatt ggctatcgag acaaggattt
    21241 tcagatatag atgtttactt attaggaagt gattataaag ttgaaaaggc aagagaactg
    21301 agttgtgaca tatttataga agataatcct ttaaactcag tgcaactagc tgatgatggt
    21361 gttaaagtaa tacttctgga tactaactat aataaagatg ttaaacatca aaatatagtt
    21421 agagttagta attggataga tattgaaaag attataaaac aaggtattta aagatttgta
    21481 gattgaaagg gggagcatat gatagattta aaaattgaaa atagaaaagg aaaaatccat
    21541 gtaaagagca gtgaaataaa agacatttta gaattacgtc cagacttcga atatgtacaa
    21601 gatatatcaa atactataaa tcaagaaaat atattggtat ttgattgcca gttgacagga
    21661 aattcttttg atatggatga tttagatata gaagaaatat tggaagagtt aggtgaagaa
    21721 atagatgagt cttatttcag tgttttattt gaagatgtaa gagcgtattt aaaagatgct
    21781 actgatgaga tagaagcaga cttacaggat aattacttat ttgataattt gagatgttat
    21841 tttgatatat acaatataaa tcaagaattt acagatttta agtttgtatt tttagtttct
    21901 tttaaagata taaaaatttc ttcattagca aacctagcta agattgtttc taagagacag
    21961 cttataggtg catctaagtt ttatagttaa aatgaaagat tattataata aaagctagta
    22021 accttgtaaa aatataggtt actagttttt tatttattca ttttcacaaa atttcagaaa
    22081 actatttaca ttctaaaaaa agtaatgtat attaaacata atgtacatta taatggataa
    22141 aaagtttcta ttaaattact aagtttattt aataatcaaa ataatttatc ttatattagt
    22201 atgtattagg gggtatttat gcaaagttta aatgaaataa ttgctgaaaa tttaaaaaag
    22261 ataaggaaag aaaaacatct tagtttagac aagattgcac aacttagtgg tgtaagtaag
    22321 agtatgctat ctcaaattga aaagtgtgag gtaaatccta ctatatctac tttgaagaaa
    22381 ataactaatg ggctaaaaat ctcatttaca tcattgatgg agaggcagga aagtgacata
    22441 gaattgattc aaaaaagtga tatagaccat tttattgaag ataatggaaa gtatataagt
    22501 tatccaatat ttccatttga ctccaaaaga agatttgaga tatttatgat tgaaatagaa
    22561 gaaggtggaa atttagattc aaatgctgaa cttccaggaa cacaagagtt tataactgtt
    22621 tttagtggcg aagtaaccat taaaataaat ggagaagatt acattgtatc tagtggaaat
    22681 tctataagat ttaaagctga tgtatcacat atatatagaa actcaggcaa aggtatagct
    22741 aaaatgagta tggtagtata ttatatataa ataatttaat ggaattagag tgatatttag
    22801 atgattttag gtatgaagat atattatatt taagttatag gagtgtgatt tatggatgaa
    22861 taataaaaaa gcagtttttt tcttttgcag tgatttaaag aaagacccag tagccagtaa
    22921 tgtgataaaa tgttgtgaag aaataatgga tttaaatcaa actgatattg ttattgatga
    22981 taattttgta ttagagttta aggataaaaa caataactta tttcattttg taaaaacaaa
    23041 agatgtaata agtcataatt ataaatatta tcttccaatt ttaaatagac attttagaga
    23101 ttatgatttt gcaggagttg taaattggca tgagggagaa aatgctccag aacatatcct
    23161 aacagctcat acaattggag atgttccaac aggagaattt ggaaattcaa atccaagata
    23221 ctttaaaaat ctaatcaatg ctattgagga tataaaaaat gaaaatctac ttgatgaatt
    23281 tactacatta acagaagcaa ctcactggtc tggaacaaca tatggtgaag aatctaaatt
    23341 aattacagaa tattctgtac ctatgttgga tattgagata ggaagtagta gtgatagttt
    23401 taataatagt atagcaatac aggtattggc aaaatcatta attagagtat ttgattgtga
    23461 tgaacctttg aagactttgt tatgtgttgg aggggtacat tttgagaaat ctttctcaga
    23521 tattataaaa aataaagaat ataacatatc aattggacat gtattgccaa atcaatggat
    23581 tgtaagtgga atgtatgatg atgaatctgg atttgagaaa ctagaaaagt gtataaatag
    23641 tattgaagga ggaattgatt gtatagtttt tcatgataaa ttaaaaggta cctataaaga
    23701 gcaatgtcgt aaactaggtg aaaaacttaa tgtacctgta tttaaacata aaatattaaa
    23761 aaatccaaaa gacttaccta tatggtagtt ttatacagtt aatataacaa taagtcggat
    23821 taatttttaa gcgaggtcaa aaatgatatt atcttatttt gcattattaa ttagagtagt
    23881 gctattagct tttgaaagag tagttgtaag attattaggt gatgaagaag gagatattta
    23941 taaaaatata gcttcatcat ttctattttt ctttataggg ggagtatgtt tacttccatt
    24001 tagcataatg gatagagtta gtgattggtc gtttttaata ccatgttata taagtagttt
    24061 agtctattca attggttcag tagcgtatgt aacgtcttta gcaactgggg aagtttcact
    24121 tgttactcca ataaatagtt taaattcatt gtttttactg cttttatcag ttatattttt
    24181 aggtgaaggt ttaagtttag caaaagttgc aggaatcata attatgattg gtggagtgtt
    24241 tatattaaaa aaagtaagct cttttttaaa gaatgtctct gtaattttta acaatttacc
    24301 atgtagatta atgtttttat acatattttt gcagtcagta ggaagagtat tggataaggc
    24361 tttttttgta caagtatctc ctatattata ttcaactata ctgtattttt tcgtaggttt
    24421 aaatttattt atctttttag catttaaaaa gaaacaaaaa ataatttgtg agatttttag
    24481 taataaaaag gggctttcaa ttttaagtgg tgctattaat ggatattctt atttagcatt
    24541 attaattgct ttaaataatc tagaacttag tatagcagag ccattttctc aagtatctat
    24601 gataataact ttaatacttg ctcattttat attcaaagaa aatataaaag aaaaaatacc
    24661 aggttcaatt ttgatactaa ttggtgggtg gctactgtta ctttaattga tataaaaaat
    24721 gaattataaa ggtgctatta gttagtatca gaaaatttaa aaaagtattg acaattcttt
    24781 taggcaaatg ttaatataat aatacaaaat agtataaata gaagacatgt tactggtaat
    24841 gcaggcagga tcgaatatat tgctttgatt tttcaaggtg tttatatagg ttctgccttt
    24901 tttgcatctt gaatggccat tcataaaaaa ttatggagaa ggagattaca agatgaatta
    24961 taaaaaaact gccattgaca ttattgaaca agttggtggt tcaaaaaata ttttagcttt
    25021 agaacactgt tcaactagac tacgctttaa gttgattgat agaagtaaag tcaatcagga
    25081 tgctttaaaa aaagtacaag gtgttatgag tatagttaat ggggcacaat ttcaagttgt
    25141 cattggaaat agtgttgttg atgtgtatga tgaaatttta aaagtttgcc ctttagatac
    25201 aaatgatagt actgtaaaaa ctgaagaaaa aataaactta ggaacaagat ttatagaatt
    25261 tattattaat atttttcaac cactgatttt tgctattgca ggagctggta ttttaaaatc
    25321 actgttgatg ttattagcaa tgtttaatat tttaaaatca gattctacta tatatacatt
    25381 attagttgct atttcagatg caacattcta tttcctacct cttatggtag ctgtaactac
    25441 tgccaatgta ttgaaatgta atcgtttagt agctattgcc gctgtaggat acttattgct
    25501 tccagcaaca acaactgctt taagtgaagg tgtcgaacta tttggattta caattccaaa
    25561 cattgcctat aatgcacagg ttttcccggc tatcttatgt gtgtctttct tggcaacaat
    25621 ggagaaaatt tttaacaagt attcaccaaa accaattcga actttctttg ttcctatgat
    25681 gtctttggca attactgttc caattactct aactatttta ggtcctttag gatataacgt
    25741 tggtacaatt tttacaacca ttattttatt cttatataac aaaatgggat tcttagttgt
    25801 aggattactt gctgcttttc ttccatttat ggttgcaaca ggaatgcaca aagcactaat
    25861 accttattca attaatacaa ttggaaagtt gggttatgaa gctttatata tgacatcttc
    25921 attagcccac aatatttctg aaagtggtgc ttgttttgca gtagccttgc gaacaaaaga
    25981 tgaaacatta aaacaaacag cattatctgc aggaatttct gccttgatgg gaattacaga
    26041 gccagcttta tatggtatta ctttacaaca taaaagagca atgttaggtg ttgtattctc
    26101 aagtgcaatt tctggtacat ttttaggatt atttgcagta aaaggatttg taattgttgg
    26161 accagggctt gccagtatga gtatgtttat tgacccaaac aatggaaaca atttgatttt
    26221 tgcaagtatt ggttttgtta ttgcaatatt aggctcgttt attattacac tgattatttg
    26281 gaaggaagat acaaatacag tagagtcatc aattggagat gttgttgaag aagagctaga
    26341 agaagaaaaa gaagtaaata tcttagtaac tccagtagaa ggaaaagtaa tagatttaag
    26401 taaggttaat gatgaactat ttgcaagtaa gactttggga gatggagttg caattatacc
    26461 gaccaatgga aatttatacg caccttgtga ttcagaagtt gtgatgttat ttgaaacaaa
    26521 acatgcaatt ggtttaagaa caaaaaatgg ggctgagatt ttgattcata ttggaataaa
    26581 tactgtaagt atgaatggag atggatttaa aacatttgtt aagacaggcg ataatgtaaa
    26641 agaaggagat ttattaattc agtttgattt agacaaaatc tcacatgcta atttagattc
    26701 aactgttatg attgtaaaca ataatggagc agattatgct tataaagtgt taaaccagtc
    26761 ttatggcaat gtaaaaaaag gaagtatctt gtttgatgta aagggaggaa tataaatatg
    26821 agaaagtttc cagaaggatt tctttgggga ggagctacag ctgctaatca atttgaaggt
    26881 ggttggaatt taggtggtaa agggtggtct gtatcagatg tagcaaaggc acactttgat
    26941 gcagatgtga aagattataa atctaataat gaaatcacaa caaaagatat agaagaagga
    27001 ttagcacatc ctgaagatga agtaaattat ccaaaacgtc atggtagtga tttttatcat
    27061 cactataaag aagatattgc tctaatggca gaaatgggat ttaagacata tcgaatgagt
    27121 attgcttggt ctcgtatttt tccaaatgga gatgataaag agccaaatga agaaggttta
    27181 cagttttatg atgatgtatt tgatgagttg ataagttatg gaattgaacc acttgttact
    27241 atgtcacatt atgaaccacc attaaatatc gttttaaact atgatggatg gtattcacga
    27301 caagttatca atatgtttgt taggtatgta gaaactatct gtgaaagata taaaaacaaa
    27361 gtgaaatatt ggcttacatt taatgaagtt gattctatga ttcgtcatcc gtacactact
    27421 ggtggtttag ttcgagaccg ttttaaggat aaaaactttg aggaagttat ttttcaagct
    27481 atgcatcatc aatttgtagc tagtgccctt gcaacaaaaa tttgtcatga aattattcca
    27541 aattcaaaag tagggtgtat gttaacaaaa ttaacgtatt atccatacac atgtagacca
    27601 gaagatgttt tggcaactca acgaaaaatg agaagtattt atgcatatag tgatacgcag
    27661 gtttttggtg agtatccagt atatttactg tcgtatttta aaaataataa cattcaaata
    27721 gtaaaagaag aacatgatga tgaaattatg aaaaagtatc cagtagattt tatttcattc
    27781 tcttactata tgtcttcttg tgaggcagct gatacaactg gattagatat tacacctggg
    27841 aatacgttat tagctgtaaa aaatccttat ttagaaatga gtgaatgggg ttggcaaatt
    27901 gattcaatag gtctacgtat atctttgatt gaattatatg atagatacag aaagccttta
    27961 tttatagttg aaaatggatt gggagccaaa gatattttaa ctgaagataa aaaagttcat
    28021 gaccaatata gaattaatta tttaaaagaa cactttaaat gtatgttaga tgcgattatt
    28081 gaagatggtg ttgaactttg gggatatact tcatggggtt gtattgattt ggtcagtgaa
    28141 tcaacgaaac aaatgtcaaa acgttatgga tatatttatg tggatgctga tgattatgga
    28201 aaggggacat ataatcgata taagaaagat agtttttatt ggtataaaaa ggtaattgaa
    28261 agtaatagta tagattttaa atgaccaata gaaggtgagc aaataaaatg aactacataa
    28321 taaagaaagt tttgaactcg agcgttgttt tagttcatga cacaaaggat aatgaattta
    28381 ttttgctagg gaaaggaatt ggatatggta aaaaaacagg agaatatatt tgtggttcca
    28441 atgataatca aatgtttgtc cctgttgaaa atacaaaatc aaaacaattt ttagaattaa
    28501 tggatgatat tcctgtcgac attttaaaga taacacagga aataatagta gaggcagaaa
    28561 agttattgaa ctcatctttt aataaaaact tatacttaat attagcagac cattttaact
    28621 ttgcaattga aaggatgcgt aaaggaataa aaattacaaa tcgagtattt tgggaaataa
    28681 aaaattatta tccaaatgag tttaaggttg gaatggttgc aatttatatg gtagaggaga
    28741 gattgggcat tcaattacca gaggaagaag cagctaacat tgctttccat tttgcaaatg
    28801 ctatggcttc agatggaaat tcatatgata cgattaagta tgccaaagta attggagaaa
    28861 taattaatat ttttgttttt tctttgaata gaaccttaga taaaaagagt atgcattata
    28921 tgagatttat aacacatata aaattttttg tggaaagatt tttttcagat agtatgttaa
    28981 gcagtggtga tgatttatta tttaatcaaa tgaaaaggtc aaatccaaaa gaaatgttaa
    29041 ttgcattaaa agttagagat tttttagaaa agaaatatgg aaaaaagtta acaaatgagg
    29101 aaatagcttt cttggttgtc catatcgcac gagttaagca atagagtgaa ttactaatat
    29161 tatcttatac gtctacatgg tagaaaattg aaagaaggta aagtatataa agaaactgag
    29221 acatattatt ttgttgttca ttatgtttat tttatgagtg tatcctgtta aaaatttaag
    29281 ttaatattta taattaaaat atacaa
//
LOCUS       DAMKMI010000036        28736 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMKMI010000036 DAMKMI010000000
VERSION     DAMKMI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28736
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.037"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            77..523
                     /locus_tag="PC429_001500"
     CDS             77..523
                     /locus_tag="PC429_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3847013.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            525..1619
                     /locus_tag="PC429_001501"
     CDS             525..1619
                     /locus_tag="PC429_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF3847014.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADRNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1707..2237
                     /locus_tag="PC429_001502"
     CDS             1707..2237
                     /locus_tag="PC429_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3847015.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            complement(3174..4550)
                     /locus_tag="PC429_001503"
     CDS             complement(3174..4550)
                     /locus_tag="PC429_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847016.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSNYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4818..5990
                     /locus_tag="PC429_001504"
     CDS             4818..5990
                     /locus_tag="PC429_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3847017.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            6004..6651
                     /locus_tag="PC429_001505"
     CDS             6004..6651
                     /locus_tag="PC429_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847018.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6653..7162
                     /locus_tag="PC429_001506"
     CDS             6653..7162
                     /locus_tag="PC429_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HDF3847019.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVLVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7526..8638)
                     /locus_tag="PC429_001507"
     CDS             complement(7526..8638)
                     /locus_tag="PC429_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF3847020.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8936..9202
                     /locus_tag="PC429_001508"
     CDS             8936..9202
                     /locus_tag="PC429_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF3847021.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9447..11174
                     /locus_tag="PC429_001509"
     CDS             9447..11174
                     /locus_tag="PC429_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847022.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11174..12964
                     /locus_tag="PC429_001510"
     CDS             11174..12964
                     /locus_tag="PC429_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847023.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13126..13434)
                     /locus_tag="PC429_001511"
     CDS             complement(13126..13434)
                     /locus_tag="PC429_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847024.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13696..14244)
                     /locus_tag="PC429_001512"
     CDS             complement(13696..14244)
                     /locus_tag="PC429_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847025.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14347..15018)
                     /locus_tag="PC429_001513"
     CDS             complement(14347..15018)
                     /locus_tag="PC429_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3847026.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15414..16319
                     /gene="garR"
                     /locus_tag="PC429_001514"
     CDS             15414..16319
                     /gene="garR"
                     /locus_tag="PC429_001514"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF3847027.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            16455..17837
                     /locus_tag="PC429_001515"
     CDS             16455..17837
                     /locus_tag="PC429_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF3847028.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            17908..19344
                     /locus_tag="PC429_001516"
     CDS             17908..19344
                     /locus_tag="PC429_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF3847029.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSEKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19530..20384)
                     /locus_tag="PC429_001517"
     CDS             complement(19530..20384)
                     /locus_tag="PC429_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847030.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20652..20975)
                     /locus_tag="PC429_001518"
     CDS             complement(20652..20975)
                     /locus_tag="PC429_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF3847031.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21178..21807
                     /locus_tag="PC429_001519"
     CDS             21178..21807
                     /locus_tag="PC429_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847032.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22150..22659
                     /gene="ruvC"
                     /locus_tag="PC429_001520"
     CDS             22150..22659
                     /gene="ruvC"
                     /locus_tag="PC429_001520"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF3847033.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            22652..23254
                     /gene="ruvA"
                     /locus_tag="PC429_001521"
     CDS             22652..23254
                     /gene="ruvA"
                     /locus_tag="PC429_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF3847034.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23361..24380
                     /gene="ruvB"
                     /locus_tag="PC429_001522"
     CDS             23361..24380
                     /gene="ruvB"
                     /locus_tag="PC429_001522"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF3847035.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            24698..25723
                     /gene="queA"
                     /locus_tag="PC429_001523"
     CDS             24698..25723
                     /gene="queA"
                     /locus_tag="PC429_001523"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF3847036.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            25735..26220
                     /locus_tag="PC429_001524"
     CDS             25735..26220
                     /locus_tag="PC429_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847037.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26299..27420
                     /gene="tgt"
                     /locus_tag="PC429_001525"
     CDS             26299..27420
                     /gene="tgt"
                     /locus_tag="PC429_001525"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF3847038.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27554..27847
                     /gene="yajC"
                     /locus_tag="PC429_001526"
     CDS             27554..27847
                     /gene="yajC"
                     /locus_tag="PC429_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF3847039.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            27905..28267
                     /locus_tag="PC429_001527"
     CDS             27905..28267
                     /locus_tag="PC429_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF3847040.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28333..28476
                     /gene="scfA"
                     /locus_tag="PC429_001528"
     CDS             28333..28476
                     /gene="scfA"
                     /locus_tag="PC429_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF3847041.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
BASE COUNT        10874 a         3316 c         4876 g         9670 t
ORIGIN      
        1 cttgactata ttaattgttt aatataatat agttgatata gtcaaacata ttataattat
       61 agaagggaaa tttaagatgg attttaaaaa tttacagtat gaaagtttgt catatattat
      121 ttgtaacttg caaaaaaact ttaaacttta ttgtgaaaaa tgtttaaaac catataaact
      181 tacgaatggt ctatatttct atttaattta tattaataaa aataggaatt gttctttgaa
      241 tgatgtttct acagaatttg aagttgataa agctcataca acaagaacta tatctaggtt
      301 agaacaagat ggatacatag aaaaaatcca aaaccctaat gatagtagag cttttcagtt
      361 gagagtaaca gataagggtg aggaagtatt aggggatata aaaaacatat tttcaaagtg
      421 ggataatcac ataaaaaaag agttctcgga tacagaatat aaggaactgg taaagaattt
      481 acatgtggta aaggatataa aaacagctgt ggaggaagaa taggatgtat tgtaatttat
      541 taaaaccaat taaaattgga aatttggaat taaaaaatag agtatctttt gcacctacaa
      601 gcatgggtct taaattggaa gaaaaaatta aaaagttttc tgatattgct aagtcaggag
      661 tagctctgat tactctagga gatgtatcta ttagacctag ttttcataag gtagctataa
      721 gcctatcgga tgaagatgga gtcatgaaat acaagaaaat tgtagatgaa atacacaatt
      781 ctggtgcaaa ggtatcagca caactatttt gttcagatta tgatgttaat ttaattaaag
      841 atactatgaa tatgggaatt acttcacatg atgagataaa aaaaataatg aatgacggag
      901 taaaagatta cataacaaat atgcccaaag aagaaattaa aaatattata aatttattta
      961 aagtaacagc attgaatgca aaaaaagcag gttttgacat gattcaaatt catggtgata
     1021 ggcttgtagg aagtttttca tcaagtatat ttaataatag aaatgatgaa tatggtagaa
     1081 cgtgtgataa tagaagtaga tttgcaagcg aaataattag tagtatccga gatgaagtta
     1141 aggacattcc aatagactat aaatttgcaa taagacaaga aaatcctcat tatggaaatg
     1201 caggagtttt actttcggaa gttgagtatt ttgttaaaaa atttgaatcc ctaggtgtaa
     1261 acagttttca tgtaacactt gcaaatcatt caaaattaga agatactata ccaaccaata
     1321 atcatcctta ctttaaagaa gaaggctgtt ttttatattt agctgatgaa gtaaagaaac
     1381 acacaaatct tccagtatgt ggggttggaa aattgtctag cccagatttt atagaaagta
     1441 ttatttcaaa taatagagta gatatggttt ctatgtcaag acaattacta gcagatagga
     1501 attggttaca gaaagtaaaa gatggaagag tagatgagat taaaaagtgc tgttattgta
     1561 ataagaagtg tgcagatgct ttacaaacac gctctcagtt tggatgtata ctagattaga
     1621 atatccttat aaaaataaat aatttgttaa ggttaatata tttttggtga tttagataag
     1681 tatttagata aagaggagga gacatcatga aaagtttagt tttatattca agtttaactg
     1741 gaaatactaa aaagattgct tatactatat atgatgaaat tcaagaagaa aaagacatta
     1801 aagatgtaaa tgaattagtt gattatagta ttgactatga aaattatgat atagtattct
     1861 taggctattg ggtggacaag ggaatttgtg ataaaaattc aaaacaagtg cttgaaaata
     1921 ttcacaataa aaagatagca ctatttggaa caatgggagc aagtaaaaaa ggaagttatg
     1981 gagctagtat aatagaaaag atagaatcaa ttatcccaaa agataatgaa atattaggtt
     2041 cattcatatg tcaaggtaag atagcagaag gattgaaagc aaaatataaa gagatgttaa
     2101 aactttctcc tgacaacgaa catattcgcc aacaattaaa taaccatgag gaatctcaaa
     2161 gtcatccaga tgaacaagaa atatatgaag ccagtatgtt tgcaaaaaat atgatgatta
     2221 aagctagtat tgtgtagtag ttaaacaata agttcatata agatatagaa taaataattt
     2281 agtaaaaaag tatagcaaat aatttgctat gcttttttta tgaaatcaaa aaaataataa
     2341 aaatacttga ctgtaaacct tgtttataca ttattttata gaaaataaga tagttttaaa
     2401 tataaaaaaa gatatattat tgaaaggaga tattattgta tgaaaactta taaaagataa
     2461 ttagtttgat tttaagatat actaaatata caattttata gagtgaaaat atttaagact
     2521 atattatagt tataaattaa tagaatagag aggtagtgtt taaattgcaa taacagtatt
     2581 tccaaagttg ccacctcaaa ttcctatcca atggaataat ggtgtagcta attatgtaga
     2641 taaaaaattt atttatgcct accctgtcat ttgtgttgta ttcagaatcc ttttacgtcc
     2701 atttatatgg agatggttac aaatacatgc tttttatagt gacactattg cagattattt
     2761 aacgaatttt atgtgttttg tagcgttgtc tatagaaata ttctctatac tttttattta
     2821 caatattgcc agacatgtta caataattat ttttatagat atacttgtat ttattgcttt
     2881 actgataata ggtatgtata aattatttaa aaaatacaat taatttttaa aaattaagta
     2941 ataagtgata gtgtaaaaag tagccactgc aaattatgga ttgtagtggc tattttatta
     3001 tttgtatatg aaattatttt gtttttctaa taagttacga tatattatgg tttatttcac
     3061 tgataaaagg tttaggtatt ttaaagtaaa aagttgtata ttctaataaa atttaaacat
     3121 tttgattctt taaaattaga gaaaatacat tgttcttatt aaaaaattaa atattaagaa
     3181 tttaatttct gcatcttatt tagaaaagat aagaaatcaa ctttatatat ttttttgggc
     3241 cttcctcttt gattatcgag ttttgtattt acaatagttg caattcctgc tttatataag
     3301 atactcaata ttctattagc acttctaaga gttatgttca tatagtttga taatatttct
     3361 gctgacacat tatttgaatt tctatcatta atcatacaca tgattttaga tacatttgta
     3421 ttagatagag gtataaaaga agcaacttta ttggctattg agctatcttc aatattatta
     3481 gaatttttat ttgaatataa agggcctatt gtatcattag tatcatttac tataaaagta
     3541 cagtttccat tcaaagaagc agatttacca ttagcttgac gagcattttg ctctgcatga
     3601 atatttgtat ttccaatgcc ccaacctata tttaagctat atttaatttc ttttaaatct
     3661 tctgaaataa aaaaatctgt atagttattg gtcatattgt ttaacatttc tttagtagtg
     3721 tgtatctcta ttttcatatc attttttttg acctgaactc cataaaatcc atgtcttgat
     3781 agagaattta atatagtgtt atatatttga ttttgtatct catcattttt aaatatcaaa
     3841 tcttcattta tgtactctga actatttact gtaactatac aagttgcaga tttatttttg
     3901 tctaagtttt gtaatgttat ttcattcacc agagatttaa atgtattcag gattgattct
     3961 gtagatggaa ccatgtaaat acaatcatat ccaagtttat taagttcaca actaatgatg
     4021 taatttctag taaaagaaag gttaatctta ttatctcgca ataagtctaa atgtatttcc
     4081 aataatttat catttaattt tttaaagtca tatacaggtt gagggatatt aaaatcttta
     4141 attgtatatg gacatttatt agagtctatt aatgaatata tatcataata attgttgtcc
     4201 tctcttaaaa aatccataaa tgattttgta aagtcaatat ctttttggct atttaataat
     4261 ttgaataagt atttataaaa atctctttca tcaagcttta aaaaatttaa tggaactttt
     4321 atgtctttta tatttattat aagttcacta tatggaacta taccactgca tacaaatcca
     4381 tcaaaattat ctacttgctc atagtatata ttaggtaaat tattaaaagg attctcccct
     4441 ttgagctcaa taaatgtaat ttcacaagta tcatttaatt ctttaagagc ttgtttaaac
     4501 ttatctgaca taaaataaga tgttgtcaaa aaaccaattt tatatttcat aaaacctcct
     4561 ttaaaatttc gagagtatag taactaatca atcaatttat aacctcattt tataatagta
     4621 gataaaacgc gtcaatttat tttacaaaag gaaaatttaa agaaaaatca gaaaatataa
     4681 aaaagactga aaaatatgtt gactgtcaag aataattgtg ctattttgta attaaggact
     4741 agtcttttaa gatttcctaa aataaactta gattttagga aagaaataca ttttatttga
     4801 aaataaagga gaagattatg aatacatatt taaatgaaat ttctaagttt gttgatgaga
     4861 atagagaaga aatagtttca ttatggaaag agattgttaa tatagaaagc tatacacatt
     4921 gtaaagaaag cgtaaataaa ttggctaaga gattaaagtt tgagtttgaa aaagaaggtt
     4981 tagattgcga gttagtagat gttggagata atggtagtac attgataggt acacttggtt
     5041 ctaatataaa taaaaagcct ataatttttt caggtcacat ggatactgtc tttgaaacag
     5101 gtacatttgg agagaatcca tttaaaataa tagaaggaaa agcatatggc ccaggtgttt
     5161 tagatatgaa gggtggtata ataatttcct tatatgttat taaagcatta aataaaatag
     5221 gatacaaaga aagaccaata aaaatagtat tttctggaga tgaagagata ggacataagg
     5281 attcaacagg tgcagatgtt atattgaggg aagcaaaagg tggtctttgt gcttttaata
     5341 tggaaactgg attagtagat aatagtttgt gtgtaggtag aaaaggaaga ataggatgta
     5401 atatacatgt aaaaggtgta gaaacacacg caggtaatga ttttgaaggt ggaagaaatg
     5461 ctatagaaga aatggctaat aagatactac gcatacaaaa attgactaat ttagatgttg
     5521 gaactacagt aagtgtaagt gtgattaagg gtggaagagt ggaaaattct attccagaag
     5581 attgtagtat aaaagtagac cttagatttg aaaaagtaga agaaatggag aatgttaaag
     5641 aacaagttag agagatatgt aaagaaactt acatagaagg aacttctaca gaagttaatt
     5701 ttataagtga aatgatgcca tttgaaacta cagaagatgt tatgagattt catacatttg
     5761 taaatgaagt gtcaaaagaa aatggatttg gagaattaaa tgctaagaga cttggaggta
     5821 gttcagatgc tagttatttg actattgcaa acgttcccgc aatctgttca tttggtgtaa
     5881 gaggggaatg gaaccacaca tcaagagaat atgcagtagt agattctatg tttgagaggg
     5941 tgaaattaat ttctacagtt attttaaact tagataaatt tgaggtataa agggggattt
     6001 tatatggatg taacagctgg agataataag gtaacatgga aagggtattt atctttattt
     6061 gtattgatag tattattttc aggagtgttt aaagatagtc aaggcttcct taaagcatta
     6121 gattttgcta atctgacagg ttcatttggt actattgcag atggtgtaga ctttatgggt
     6181 aaaggaggaa cgggagcaaa agatggtttc ttatttgctc tgactttgat tccaactgtc
     6241 tgttttgctg taggtcttat agatgtagtt gaatctatgg gaggaatgaa agcggcatca
     6301 aagatattta atccattgct tagacctctg cttggtatac ctggaatagc aggaatagca
     6361 tttgtatctt catttactag ttcagatgta gcctcaataa tgacgaaaga attatttgaa
     6421 agtggggaaa taacagatga tgaaagaact atatttgtag catatcaata tgctggttca
     6481 gctgttatac tgaacacaat aaatactcaa gctccacttt taccaatagc attattggca
     6541 ttaggtccaa taatccttat agagatattc tgtaaaatac ttggtgctaa tctagttaga
     6601 ttaatcatag gtgtaagaaa taaaaagaat aaggctaagg aggctgtgta gtatgagtca
     6661 acaaaatact ggaaatgcaa ttgtaaatag taaacctaag aaaactgctg ttgagatatt
     6721 tatgggggga gcaaaaaaag gtttttacat aggtgttgaa cagatattac cagccatgat
     6781 aatgggttat gtaatagtgc agtttttaca attaactggt ttagttgata ttttaggaaa
     6841 tatttttgga ccagtaatgg gtatttttgg actacctggg gaatctgttt tagttttgat
     6901 agcagcattc tttgctaagg cagcaggagc agctacagct gcaaacttat ttgcacaagg
     6961 tctaatcact gcaccacaag caaccatatt aattatgcca tgtatgttaa tgggtactct
     7021 agttggtcat tatgccagaa tagtgcttgt agcaaatgcc agtactaagt atagaatgtt
     7081 actgttggca gttcctatat ttgatgcaat agtggggatg ttaatagtaa gagttttact
     7141 cactgttatg ggattaatgt aaataacata aaattataac ctaactataa atgatatata
     7201 agaattaata tatacaagga ttaatgagta gtaattgata tatagtaata aactaagata
     7261 gtaattaatg tgtatattaa ttaaaaaaag agagtatctc aaagtgaact ttttagttca
     7321 tgagatactc tttttgtatg aaatcaagta tattttcata atttcaacaa tgaaagagag
     7381 gcttatctct attttgagac agcctcttct tttaaattta tatttctaga atttatgttt
     7441 tttatttatc caacattaac attagttatt agaaataatt ttaagaaaaa tatactttca
     7501 tgttaatatc agaataaatc tgtttttaaa ggatgatttg ttttgaattt tcacgaatat
     7561 ctacttctat gactttttct attgtatctc ctctaaaacc taataaatta cttgtcaagt
     7621 ttgcagtgga acaagaatga ttaggtataa ttcttattct atcaccaatt tttatatcag
     7681 tttcaccatt tactttaatt tttccaactt cttctgaaag tgaatcaata gtaagttctg
     7741 gatgtccaca taccctacca taaccaacaa tactactatt tccatgtgca cctttatcaa
     7801 gtccaaggca cttacttcca caatcaatta aatagtatcc actgctattt tttgagatta
     7861 cagtgcacac aacagtaaga gcacacatat cttcagtagc acaacctaaa cctatctgta
     7921 tagcatcata gtatacatag tttccaggtc ttacagcagt tatagtttta gaagcagctt
     7981 cagattcaac tgttggagta ctaccagaag cgacaatttc tatattaaat ccattatcag
     8041 ttagagcttt gtatgctttc tccatagaag aatcttcttg aacacaaaca tctggaacac
     8101 cttctggaga actacaacca tacacttgac ctggatgcga acttatacca acaatcttta
     8161 tgttttctag cttagaaatt tcagtagcaa gttcaaccac tttctctggt tcaaccccaa
     8221 aacggtgtaa gccacaatct acaataataa gtagcttgca ctctattttt gatttagaaa
     8281 aagcttcact ataagcctta gcacattcta tattatctgt agcacagatt aaatttattt
     8341 ttgatgccat agtcacaatt ctatttatat ttacttctcc catgtaagga taagccaaca
     8401 taatatcttc aaaacctgct tttatcagag cttcaccttc atcaagtgtt ccaactagaa
     8461 aaccactagc acctgcattt ttttgaagtc tcgctatgta agtactttta tgtgtcttaa
     8521 gcataggcca aagttgttta ttattatttg tacaaatttg ttgaatttta tctatattct
     8581 tttctaactg gtctaaatct agcaaaaaat ttggagtagc gacttctgaa attttcatgt
     8641 tatttaccac ctttcaacaa aagtaaatta actataagta ttataattat tatattagta
     8701 atatcgtctt tataatatta atagcttata attatattat agaactagcg ttaacagaga
     8761 gcaatattta tttgtaataa ttagaatttt caataagtta gtgtggtttt aaattattta
     8821 tataatatat ttttaatttt atgcaaagtt tatttaaatt agtttgagga tttattaata
     8881 gggtattatt gaatgagtaa aaaagtgatg tttacataga ataatcacac tataaattaa
     8941 tttagaacct aaaaaattaa ttgagcaaat tggaaaagag aaggataaaa aagatatttg
     9001 gatagttggt ggaggcgata tagtaaaatt atttatgaaa gataacttaa ttgatgatta
     9061 ttatatatat atattgccaa taatattggg gagagggatt tcactattta agtctggtat
     9121 agataagact aatttgaatt ttaagaaagc tagtaatatt ggagaacttg taaaattgga
     9181 atacagtaaa aaacaaaaat aaattacatg ccaatttgtt acaatgttat gaaatgaagc
     9241 atttaaaaga cttttggcta tatgaattga cattacttgt caaatatgtt aacatataag
     9301 cgacttgtcg gagggtgtat taaaaagaat atgctggaga agagagtgcg ttaactcttt
     9361 tctccttttt ttataaaatt aagcatttat aaatttagta ttattacatg attacactat
     9421 ctcaaaaatt aagaaaggag aaaaatatga aattaatttt aagttattta aagaattata
     9481 agttattaat agtattaaac atactggcta tatttagttt tgcattagtc gagttgggga
     9541 ttccaactat aatagctaaa ataattgaca atggtattgc aaatcagaat atagcttaca
     9601 taaaacaaat gggaatagtg attgttatta tttctattat aggggttgta ggtagtatat
     9661 tgttgggtta ttgttctgct aaggtatcaa caagtgtaac cagagatata agaaatgaca
     9721 tatttgagaa atcacaagaa ttttcacata ctgaatacaa taaatttgga atttcttcaa
     9781 tgattacaag aacaacgaat gatgcatttc aaattcaaca atttgttaat atactactaa
     9841 ggactgcctt attaactcca gttatgttca ttataagtat aataatgaca attagaacaa
     9901 gtgtggaact ttctatagta ttggctatat cagtaccttt tataattatt ggagtagcaa
     9961 taatagcaaa agtatctcag gcaatatctt caaaacaaca aaaaggacta gataaattaa
    10021 accttatatc gagagaaaat ttaactggaa taagagtaat tagagctttt ggaaatgatg
    10081 attatgaaac agagcgattt gaaaaaacaa atacatatta tgctaatgtt tctaagaaac
    10141 tatttaagct tatgtcaatt actcaaccag cattcttttt actattaaat atagctgttt
    10201 tggctgtatt ttggatttca agtgagaaga taaatattgg agagcttcaa gttggacagt
    10261 tggttgcatt tttggaatat cttttccatg ccatgttttc aattatgctt ttttcaatgg
    10321 tatttattat gtatccaaga gcagaggtat ctgcaaatag aataaaagaa cttttaaatg
    10381 aagaaccatt gataaaaaat cctgaaaatg gaattaagga tactgaaaat aagggtatca
    10441 tagaatttga taatgtaact tttacctatc cagatggaga agcttctgta cttaaggata
    10501 tttcatttac tgcaaaaaca ggtgaaacag ttgcttttat aggaagtact ggtagtggga
    10561 aaagtactct aattaacttg atacctagat tttatgatgt aacagaaggt agtattaaga
    10621 taaatggagt agatataaga gaatatgatt taaaagcttt aagaaaaaag ataggattca
    10681 tacctcaaaa atcactgctt tttactggta gcatagcaaa taatattaga tttgggaaac
    10741 ataaggcagg agagtcagaa ttagaatatt ccgcaaaagt tgcacaagca tatgagttta
    10801 tatcaaaaaa acctagaaag tttgatgaat tgattagtga aggtggagca aatgtatcag
    10861 gaggtcaaaa acaaagactt tctattgcaa gggctattat aagaagacct gaaatataca
    10921 tttttgatga tagtttttct gcacttgact ttaaaactga tgcaatactt cgtgctaaat
    10981 tgaagaagga aacaaaagat gcaattgtat tgattgttgc ccagagaatt agttctatta
    11041 ttgatgctga taagattata gtactcaatg aaggacaagt tgtaggaatg ggaactcata
    11101 aagaattatt aaaaaactgt gaaatatact atgaaattgc aacatctcag ttaaaaaagg
    11161 aggaattaga ataatgaaaa agtctaaagg ttttaagaaa acaattggta gacttcttcc
    11221 atttgtaaag aaatataaat tttcctttat tatagctatt atatgtataa tttcagcagc
    11281 tactatgaat gcacttgcac ctaaaactga aggattgata ataacacaat tgactaaaga
    11341 tgtaattagt atcgcaaaag gagtaccagg ggcttccgta aattttgatt atgtaacaaa
    11401 aatattggtg attttagcat gtatatattt tgccaatgca atttttacgt atacttcaag
    11461 ttttctactt accaatgcta ttcaaaacac aatgagagat ttaagaaatg aagtcgaaaa
    11521 taaaataaga agattgccta ttagttattt tgatagtaat agttttggag atgtattaag
    11581 tcgtatatca aatgatgtag atacaatatc aaatgcattg cagcagagtt ttatgcaaat
    11641 tgtaaactca atcttagtca taattttggc actttcaatg atgtttacaa ttaatatata
    11701 tatggctctt atagctcttt ttataattcc aattagttat tttgtatcta aatttgtagt
    11761 aaaaaaatct caaagtagat tttcattgca acaaaatgct cttggaaaat taaatgggaa
    11821 agtacaagaa atgtatactg gatttaatga aataaaacta tatggtaaag aagaagattc
    11881 tataaaagaa tttaaaaaag taaatcaaga actttgtgaa aatggattta aggcacaatt
    11941 tatatcaagt atgatgaatc ctatggtttc attagtaact tactttggaa ttgcagctgt
    12001 ggcagttgtc ggttctatat atgcagtaag tggaggaata acagtaggaa atttacaagc
    12061 atttgtgcgt tatatatggc agataaatca gccactttca caaatgactc agttatctac
    12121 agtaatacaa tcttcttttg cagctataga acgtgtattt gaaattttag acgaagaaga
    12181 ggaaatacct gatgttgaaa atccagttaa gatagaaaat gtaaaaggaa atgttacatt
    12241 tgagcatgtt aattttggat atggagaaaa ttcaacttta atagaagatt taaatgcaga
    12301 agtaaaaagt ggtcagatgg tagctattgt tggacctact ggagcaggta aaacaacatt
    12361 aataaactta ttgatgaggt tttatgatgt aaagaaaggt gctattaaaa ttgatggagt
    12421 agatataaga gatatgaaac gtaaagattt gcgttcaatg tttggtatgg tgcttcaaga
    12481 tacatggtta tttaatggaa ccatttttga aaatatagaa tatggtagat ttggagctac
    12541 taaagaagaa attatacaag cagcaaaagt agcaaatgtt catcacttta taaccactct
    12601 tccagatgga tacaatatgt ttttaaatga agaagcatct aatatatctc taggggaaaa
    12661 acagctttta accatagcta gagcttttat atcagaccct tcaatattga tacttgatga
    12721 agctacgagt tctgttgata ctaggctgga acttatgctt caaaaagcaa tgagaaatct
    12781 tatgaatggt agaactagtt ttgttatagc acatagactt tctacaattc gcaatgctga
    12841 cttgatttta gttatgaata atggaagtat tatagagcag gggaatcatg atgaattaat
    12901 ggaaaagggt ggattttatg agaagcttta taatagccaa tttgcagaca aagaatcaga
    12961 atagataaca taaacttttg tataaaattt tgtatcaaat tcaaatatta atcatataat
    13021 taaaaagagt atcagtaagt tgttttttta tagatttact gatactcttt gattttaaga
    13081 ccaaaggtac ttatgattgg tacttataat tcacaactag tcttgttaca tctgataacc
    13141 agaattacag tacttttctt ttttaccttt ttgttctcta acatataaca attcgcttaa
    13201 cttatcaatg taataaccat catcacttat gcgaattaaa ctagtcagat aatatccatc
    13261 tttgcttcta ggaatctttt tatttattgc taaatcaata gctgtctttg ctgcttcaaa
    13321 atgctttaaa tgtgtatgac cttcttcaaa actctttcta gtattataaa ctacataacc
    13381 ttttttaact tgaaaaacta taaattcttt cttttcatat attttattaa acattcaatc
    13441 agcccttctc aagatagttt tatagcataa cattagaagc ttctaaaaaa ttttacaaag
    13501 aacataaata ttttataaat tataaatcta aaactataat ttataaagat aactttctga
    13561 ttatttatat gataattcat atagattaaa agtaaattat ctttaggtta aacaaaagta
    13621 aaataaaaga ctagttataa tatttatatg aaaaagtaca aaatatagta tatgaatata
    13681 gaaaatttaa attatttatt tacagttttt atttccataa gtatatttac cttcagatac
    13741 cttgtcatca tggaaatata ttctttcaca acttttatct ccccatagaa tgttttttgt
    13801 tattttatgg gacgtatctc ctgtaaatac ttcagctaca gtagtatctg taacatcaca
    13861 gtaaagagaa gcgaattttt taaaccaagg attttcctca aatcttaaaa gccaggaagc
    13921 tccatagggg caatgtaact cttcaaattc aggaacccat cctagaaatg gaacatcagt
    13981 tgtgctaata atattgtctg caattaattc atctccattt tcatgagctc tctctctcat
    14041 agcaatagct ctttcttcag acctttcaaa taaaactttt cttaatattt cgctagtttt
    14101 atcttcacca aactcttcaa ataacacttt ggaaaaataa aaatataaat caccaaaatg
    14161 tttgcatgcc atacgaactt cttcaatagc ataatctttg tcaatagttt cattagttac
    14221 aacatcttcg ttcaaaaaaa tcactcctta gaataagtat tagttacaat ataattatag
    14281 aatatttttt ataaattagg aaattaattt gagatagata tatttttaat aaatgaaagt
    14341 ataatgctaa aataacttat tgttacaaaa atcttctaaa ttaatgtatt taggagtttc
    14401 catatgaatg agagtagttc tgtaaatatc ttcaatacat ccatttgcaa tattattaaa
    14461 aatatctgtg tgctctttta atgttgtttc aagccttcct tcatgtgaca atgaatcgag
    14521 agctaatttt tttattctgt acatataatt gttaaacatt ttgtcaaatt cagtattttg
    14581 agcatatgaa actatagttg tgtgaaataa tgtatcgtat tctgcaaatg agtttaaatt
    14641 tttatcacct aataaaatat ctttttgttt atctaaaaga tgttttaatt tacttatagt
    14701 ttctactgct ctagcttctt tgtattgact tgtaattaaa aaagtacaaa atccttctat
    14761 tgcagagcgt atttggaata tctctacaat ttcatttgct gtaatttgat ggattctaaa
    14821 tcctttgctt gggattatat ctataaatcc atcttgacta agtctttgaa gagcatctct
    14881 tataggtgtt ctagagatac ctatttcact agcaagtttt gtttcagaat atatttcatt
    14941 ttctatcatt tctccagata gtatcaattc ttttaagtag tcatatgctt gttcttgtaa
    15001 tcttttaaat gtagccatac ttaataatac ccccctaaac aatataaaaa tacaaaaact
    15061 tatataattt taagcataca acatagtgaa aataaggtca atcttttgca atttaaattg
    15121 cagataagat taaaaaatat aaaaaattat tgaagtgtga agcatgatta acattaaagg
    15181 cagattaatt aaaactaatt gaaattagtt aaaatacaaa actgtaaaag tagtatatta
    15241 tatttgaaaa caatagtatg tattaaaact tatataggat gactaaatat aataaatgac
    15301 tttaaaaatt actatttatt gttaaaataa atacttttat attttatatt gaccggtata
    15361 ctggtatgta gtatactcta aaacataaaa aacgttttca aaaatgtgaa gatatggagg
    15421 gttataaaat gaaattggga tttattggat taggaataat gggaaagcca atggcaaaaa
    15481 atttattaaa agatggatgt aatcttttgg tgtatgacat taataaatct gctgtggatg
    15541 aacttatatc atgtggagca aaatacgcaa gtgttttaga aatgggtcaa gaatgtgata
    15601 ttgtatttac aatccttcca aatggaacta ttgtgcagga tatattgttt gggatggatg
    15661 gattggcaaa aactttaaaa gaaggaagta tagtagtcga tatgagttca gttactccta
    15721 cagaatccat attgtgtgca aataaattaa aagatatggg cttagaattt atagactcac
    15781 cagtaagtgg aggggagcca aaagcgattg atggtacact tgcttttatg gctggtggta
    15841 aggaagaaat ttataagaaa gtagaaccat tttttaatat aatgggaagc tcatctattt
    15901 taattggaga taatggtagt ggaagtgtta caaaacttac aaaccaagta attgttaatt
    15961 tgacgatagc agcagtttct gaggcatttg tacttgcagc aaaagcaggt gcagacccag
    16021 aaaaagtata taaggcaata agaggaggcc tagctggtag tacaatattg gatgcgaaga
    16081 taccaatgat tatgaatcgt gattttaaac caggtgggaa aatatctatt aatttgaaag
    16141 atattaagaa tgtaatgcaa acagcacaca atttagatgt accactacct atgacaagtc
    16201 aattattaga aattatgcag acattaaaag tacatggaca tttggaagat gaccatagtg
    16261 gaatagcaca gtattttgaa aagttggcag gtgttgaaat aaaaaaacac aataattaat
    16321 atttaaatat atcaatataa ttaaatacat attattagga ttagattata taaatacagc
    16381 gaggttaagt tatcatagta aaagaaaaat aaaattgata aataataaag tagaatgata
    16441 aaggggtaaa gattatgatt acaggatatg gacttttgat agcatttgtt atatctatag
    16501 gaattttact tgtatcgatt attaaattta aagtaaatcc gtttttggca ttactgataa
    16561 catctataat tacaggcttt atggttagaa tgcctataaa tgaaatatca accacaatat
    16621 cgactggatt tggaaataca ttaggtagta taggtattgt aattggactt ggaataatat
    16681 ttggaaatat tctttcagaa tcaagagcaa cagaatctat tgcaaagggg ttgttagcta
    16741 agactggaga aaaaaacagt gctttagcag taactacagc aggtttttta atttcaattc
    16801 cagtttttat ggatgcagct tttgttataa tgatgcctat aattaagtat gtttcgagag
    16861 taactaagaa atctctaatg gtttttgtat gtgccttagg agttgggact atagtaggtc
    16921 atgcacttgt aatacctaca ccaggacctt tagcagttgc agcaaatgta aatgcaaatg
    16981 taggtagttt tatactttat tctataattg ttgcttttcc agcagctctt ataggtggat
    17041 ggatttatgg aaaacgtttt gaaaaatatc cagcttatgc tattgatgag aatgatagag
    17101 aaaaaaattt agagcaggga aaagatagca taaaaattga agatgatgat tcttctaaag
    17161 taccaggttt tggaatttct atgttttctt tattgttccc aatacttctt atacttatat
    17221 caaatgtatt tagtatgttc ttagaaaaag gttctacaat gagtggagta cttgcattta
    17281 taggagataa aaacatagca atacttcttg gaatattggt ggcaatagga tttttaaaga
    17341 aatacataaa taaacctatg ggagatgtag ttattgaagc tgctgattca gcagggctaa
    17401 tattattgat aacagggtca ggtggtgcgt ttggcagtgt tataaatgca agtggtatag
    17461 gtaacttctt agttgatact atgtctggtc ttagtatatc agttgtagtg cttggattct
    17521 tattaagtgc cttgcttaga atttctcaag gttctgctac tgtagcctta gtaacaacat
    17581 catcaatttt aggacctaca attttggcaa ctggaatgtc accagtttta gtagggcttg
    17641 ctatatgtgc tggaggagtt ggtttctccc ttccaaatga ttctggattc tgggtacttt
    17701 caagatttag tggattatca gttaaagata ctttaaactc ttggacaatt ggaggaacca
    17761 ttgctggagt tgtagcattt ataatggttt tattgcttag tgttataaat ggaattatac
    17821 cacttccagg tctataaata acaaactatt gtttatatca tataaattac atttagacta
    17881 tacaaattac ataaaggggc gttttaaatg agagaagtaa aaccaattag catagatata
    17941 cttaatactt ttaaacaagt agatgaggac agattaaata aacttcttgc agatgagctt
    18001 aagcatttag atagaaaaat tgttgtatta gatgatgacc caacaggtgt acaaacagtt
    18061 catgatattt ctgtttatac tgattgggat aaggatagta tggaacaagg ttttaatgag
    18121 aaaaatagta tgttttttat acttaccaat tcaaggggat ttactgttgc tcaaactaca
    18181 aaagcacata aagaaatttc aaagaatatt gtggatgtat caaagaaagt taataaggat
    18241 tttataatca taagtcgtag tgattctact atgagaggac attatcctct tgaaactaat
    18301 ttattaaaaa gcgaagtaga gagattatcg gagaagttat ttcagggtga aataataatg
    18361 ccatttttta aagaaggtgg taggtttact atagataatg tacattatgt taaagaagga
    18421 gaaatacttg taccagcagg aatgactgaa tttgcaaaag ataaatcttt tggatataaa
    18481 tcatctgata taggtgagtg gtgtgaagaa aagacaaatg gaaagtacaa atctagtgat
    18541 atgatatata tttcgtcaaa agaacttaga gagctaaata ttgaagctat aacagaaaaa
    18601 cttaaaaaag ctgaaggatt caataaaatt attgtaaatg caaccgatta tgtagatgta
    18661 aaggtattta caattgcgtt tatacgagca gtaaatagtg gtaaggaatt tatgtttaga
    18721 agcgctgctg ccataacaaa aatacttggc ggagtgagtg acaaggaact acttactaag
    18781 gatgaattag tatcaaaagg taataccaat ggaggaatta tacttgttgg ttcacatgtg
    18841 aataagacta cccaacagtt agaagaactt aaaaattgta aatatccgat agaatttata
    18901 gaatttaatc aacatttagt tcttcaagaa aatggtctaa aaaatgaggt aaaaagagtt
    18961 ataaaagttg tagaggaaaa aataagcagg ggtaaaactg ttgctgttta tactagacga
    19021 gagcgttttg acttggatac gaatgataaa gataaacagc ttatgatttc tgtagaaata
    19081 tcagatgcag ttaccagtat aattggtatg cttaatgtaa gaccaaattt tattatagct
    19141 aagggtggaa ttacttctag tgatgttggt actaaagcac taagagtaaa aaaagctaca
    19201 gtaatgggac agataaaacc tggtatacca gtatggatga caggcaaaga aagcaaattt
    19261 cctaatatgc catatataat tttccctgga aatgtgggag agattagcac acttagagaa
    19321 tcagttgaga tattgatggg gtagtatatt agtgtattga agtttaaacg cttaaattta
    19381 ctgagttaaa attaaaataa actaaaatta taaattaaga ttaaaaataa tagtatatta
    19441 attagggttt agagtatttt acaaaaaata ttctaaacct taatcttatg tattagtatt
    19501 tttattattt aacttatgtg tttattttat tatttatctt ttttaattga tttaatattt
    19561 tctttgattt tatttaaaat atctgtatgc ttattagagc ttatgttggg gaatgctttg
    19621 attatacgtc tatgaagtaa tttgtggtta gacagcaaag tattaagttt attttttagc
    19681 tcatcaatac gatttttagc atagcgataa ttttcttttt ctttaagtat ttcaaaccta
    19741 tcttccaaag tatccagtct attttcaact aaagattcaa gtttgttttc tatagtttct
    19801 tgtgtaataa gtttgtagta ttctttctta tcttttaatt ccttaattat aagattgatt
    19861 tgttttaact taccatttaa ttgtaaaatt gtatggactg tgataactaa atctaaaaca
    19921 aaataaattt ctatagatat agcaaatata aatagcacat ttttagagat agagttaata
    19981 aagtcaacca caataggatg tactataagc attacaatca atgataatac accaaacata
    20041 agtgagtttt ccagacaaac tcttccgtga atattaaatt tatgtttaga ataatcccac
    20101 caagtcatat taaatatttt ttctaaaata aagctcgtac aatactccat tatgctagtt
    20161 ataattatac taaacaaaaa caacaaagga atagtgtgta caaaaggagt caaggtagca
    20221 actattgcta atgctccaaa tccatatata gggcaaaatg gaccatttaa aaaacctcta
    20281 tttacaaatt gtttttgtaa aactgaacaa tatgtagttt cacaacacca tcctataaag
    20341 ctataaatta caaaataaag aaattgaata taaaatgtac tcataagcac ctcatttcta
    20401 gttagtataa cactatatac tcgatatttt atttttatga ttaatttact aaattcaatt
    20461 tacatattga ttataccaat ttatagaaat ttatcaaatt taattacagt tagaacatta
    20521 taaacatggt tataataatg aaattttatt taaaataaat aagggtgtca agtataatca
    20581 aactaatagt tgtagatact ctacactatg ttagtttaat tacatttgac acccttatat
    20641 ttatactatg actatttaca ttcgaaacta gagcaacatg tttcaatata aaggtttact
    20701 ctatcagttt caggagcaac caatatatta tcaagtgtac ataagctacc atctttgttg
    20761 tatttacaat tttttacagt acaaactaat tcctcacaat ttctattatt aacgttgcta
    20821 gataaattac tataaataga ttcatctaaa aatgaactac aacaagtatg tctgtcattg
    20881 tgagcttttt caccatttat aaaaatttta tctgcataac atatatggct gttgttatgt
    20941 gaacagttaa ttacattaca gtttattctt tccaaagtta ttacctccta aacgattatt
    21001 tattataaat tatgtccata taattacaag aatataacca ttaagagaaa actacctttt
    21061 agaaaatatc tattcttaga gtaatttgaa atataaatat attttcttct aaaatttacc
    21121 taaattgtat aaaaacttac agcttggaga aaataataga aaaaggaggc gtaatcaatg
    21181 tttgaaaaga agaaaaaaga attatttcct tggaaaatat ctgccttaat actggttgga
    21241 gtatttgtat taagtagcgg agtttatata gggacaagat taaaacacaa tggagttcaa
    21301 actggtgaag ttgtaagtac tagtgcgaat atgaatgaaa agtcaaaaga agcttttgga
    21361 ttaaataaaa attgtgaagt ttggcttcaa agtgaaggcg aaaatgaaac taactataag
    21421 agaacactta caatggcagg aatagttcca gagtcattat tagataagac gaagtcagaa
    21481 atagtatcat attttaaaaa agaatatcca actaaaaaaa taaagagtat ggataaaaat
    21541 gaaatcatat tggtagaatc taaatatagt gataataaag atgtacaagc aatggatgtt
    21601 actataaaag gtaaatatac tattgaaaat gataatggaa atatagcact atataaatat
    21661 gatgatagtg gaagcaaaac attagtagaa aaaacaaaaa taagagtaga ttcacttcct
    21721 aaaacagttc aagatgaaat caaaaaaggt gtagttatgg atactgaaga agaggcctat
    21781 tcaagattag aagactttgc aagttaatag taaattaata ttattttaag ttaaatacta
    21841 agtaagttaa atactaagta aattaataat aaataattta atattaaata gattagtatt
    21901 aaatagatta gtattaaaat tgtagtcaca tttaattcct ataaaaatac aataatttat
    21961 ttgtaataaa tataaaagtt atattgaggt aaaatcttgc tttgatataa cttttttatt
    22021 tgattttata ttttacatag agcattttat gatttgttaa tattatttat gatataatga
    22081 tttagaacat aagtttttat gagaaataca ttttggtatt tcttttattt ttagggggaa
    22141 ttttaaaaaa tgataatatt ggggattgac ccaggtatag ccatagttgg atatggtata
    22201 attgaataca aaaatagcaa gtttaaagta atcgattatg gagcagttac aacacctgcc
    22261 catatgaata tatcgagaag attggaactt gtgtataaag gaattgatac aatagtaaag
    22321 aattacaata tagatgaagt tggaatggaa gaattattct ttaacaagaa tgtaaaaaca
    22381 gctataacag ttgcacaagc tagaggtgtt actatgcttg catgtgctca taatgggaag
    22441 cctgtatatg aatacactcc acttcaagta aaacaaggtg tagttggata tgggagagca
    22501 gataaggcac aagttcaaca gatggtaact tcatttttaa gtctaaaaaa agttccaaaa
    22561 ccagatgatg ttgcagatgc tttagctgtg gctatttgcc atgctcattc aaacaaactt
    22621 gaaaaaactc taaagaatat aggtggtaag tatgtatagt tatataaaag gtacagtaga
    22681 agaaatttat atagacagta tagttgttga aaataatggg ataggatata aaataaatgt
    22741 atcatccaat acaataatga atttacaagt gggagaagcc acaaaaatat atactaaatt
    22801 aattgtaaga gaagatgata tgagtctttg tggttttgta agtagggaag aacttaagat
    22861 gtttgaatta ctaacatctg tatctaaaat aggacctaaa gtggcgcttt caatactatc
    22921 atttgcatca ccagcacagt taggagcata tattttaagt gaagacattg gaaaattatc
    22981 caaagctcct ggtgttggta aaaagacagc agaaagaatc gtcttagagc ttaaggataa
    23041 ggtagacaaa aataacatag agtttgaacc aacattatta tcacaaaaac ctaccttgat
    23101 atcacaagat gagtctgtag atgcacttgt agctttaggc tatactttgt cggaatcaaa
    23161 agaggcagtt caaaaatgta aaaaagatgg tatgaataca gaagctatta taaagaaagc
    23221 acttacatat ataatgagta aatccttgaa atagtatttg gattttataa tatttggatt
    23281 ttaaaacaat atttaaggag tttatgtaaa tttgaaaata tataaagtag attttcaaaa
    23341 gtatgagagg aggggtataa atgcaaggtt ttgaagatga aaataggata ataacttcta
    23401 caatgaagat ggaagatata gatatagaaa atagtttaag accaaaaact ttagaagatt
    23461 atttgggtca agaaaaatcc aaagaacaac ttagtatatt tatagaagca gctaaatcta
    23521 gaaatgagca gttagaccat gtgcttttat atggaccacc aggtcttgga aaaactacac
    23581 tagcaagtat aatagcaaat gagatggggg taaatttaag aattacgtca ggacctgcaa
    23641 tagaaagagc tggagatttg gcagccatac ttacgaattt aaatgaaaat gatgttttat
    23701 ttatagatga gattcataga atcaatagaa gtgttgaaga agttttatat ccagcaatgg
    23761 aggatttttg cctagatata ataataggga aagggccatc tgctagaagc ataagacttg
    23821 accttcctaa atttacactt attggagcta ctacaagagc tggtatgctt acaaatccac
    23881 ttagagatag atttggtgtt atttgtaaat tggactatta tacagttgat gaactttcaa
    23941 aaatagtact taggtcttca agcatcttag atgcagagat acagtctaat ggagcattag
    24001 aacttgcaaa acgttcaaga gggactccaa ggatagcaaa tagattacta aaaagagtta
    24061 gagattttgc acaagtcaga gctgatggaa aaataactga taaggtggca aaagatgctc
    24121 tggaactttt aggagtcgat agtttgggtc tcgattttgt agatgagaag cttcttatga
    24181 ctataataga aaaatttaga ggaggaccag tgggtttaga tacattggca gcttctatcg
    24241 gagaagatag aaatacaata gaagatgtat atgagccata tttgcttcaa ttaggattta
    24301 taaatagagg tcctagagga agagtagcta tgccattagc atatgaacac ttaaaaattc
    24361 catatcctaa tgaaaaataa aaatagatta tacgagtatt tataaaataa aagagtcatt
    24421 aaattaaaat atttctaaaa tggctaaaaa ttcaaacata tccacaaaat ggtaattata
    24481 tctgtagata ctgtggataa acacgataaa aattgtgtgt attgtatcta tatattgtgg
    24541 ataagacatg tatatcgtgg aaaaatatct atatattgtg gatatggtgt ctgttaaaaa
    24601 tagcaatttc ttgatagatt atggtaatat tgtggaaaat aagttgtttt tttgatatta
    24661 ttgtagctgg agaaatttta agagaggaag tttaacattg aaaacaagtg attttaaatt
    24721 tgacttacca caggaactta tagctcaagt tcctatagaa gatagagcta gctcaagatt
    24781 gatggtatta gataaagaga caggtaatat tgaacataaa gtatttagag atataataga
    24841 atatttaaat ccaggagatt gcttagtatt aaataatact agagttatac cagctagatt
    24901 aataggagag aagcttgaaa ctggaggtaa aatagagttt ttattattaa aaagaacaga
    24961 ggaagatact tggcaggctt tagtaaaacc aggaaaaaga gcaaaagtag ggactaaatt
    25021 ttcttttgga aatggaaagc taattggaga agttgtagat ttatcagatg aaggttcaag
    25081 aattataaaa ttccattatg atggaatttt tgaagaaata ttggatgaac ttggaaatat
    25141 gccactacct ccatatatca ctgctagact agatgaaaaa gaaagatacc agactgttta
    25201 ttcaaagcat aatggttcag cagcagcacc tacagcagga ttacatttta cagaagaatt
    25261 gttaaataaa ataaaagaaa aaggtgtaga tatagccttt gtaactctac atgttggctt
    25321 aggaactttt agaccagtaa aagtagagga tgttttaaat cacaagatgc attcagaata
    25381 ttatatggtt agccaagaag cagctgacaa aataaataga gcaaaggaaa atggaaaaaa
    25441 tgtaatatgt gttggtacta ccagttgtag aactatagaa tcagcttgta atgagtatgg
    25501 aaagatgaaa gaaactagtg gatggacaga aatatttata tatccaggtt ataaatttaa
    25561 agttttagat aaacttatta ctaattttca cttaccagag tctacactaa taatgttagt
    25621 aagtgctatt tgtggaaagg ataatgtttt aaatgcatat aatgaggcag taaaagaaag
    25681 atatagattc ttcagttttg gagatgctat gattataaag tagggaggta atggatgcat
    25741 agtttacaaa aacaaaaaaa gaataaccct atattaaaga ttgcttttat atttgaacgt
    25801 gttttagcag tagtagttct aatagcagta tttttaggaa ctatagatgt tctaagactt
    25861 atgtggggag catatatagt tgattttcaa aatccagtac aatacagtca gcttaatgat
    25921 tttttagcac aaatattact tttagtaata ggtgttgaac ttgtagtaat gctatctctt
    25981 catataccag gagcatttat tgaggcatta ctttacgcaa ttgctagaaa aatgttgcta
    26041 ctgcctaaaa atgaaggtat gatagatgtt ttaataggag ttatagctat agcaggacta
    26101 tttgctataa aaaagttttt agtaacaaaa gacaagagtg ctcttaatat aatgagtatt
    26161 catgatgaag aagcaattaa ggaacaacag aacaaagaag acgaagataa atctatttag
    26221 acaataaatt atatgaatag cttattacaa atatatctta agaaataatg agaaataaat
    26281 atgattaagg agaactttat gtacgcagta agatacgaat tgataaagac ttgtaagcag
    26341 agtggtgcaa ggttaggaag attacacact ccacatggga ttattgaaac accaatattc
    26401 atgccagttg gaactcaagc aactgtaaaa tctatgacac cagaagaatt aaaagagatt
    26461 ggttcacaaa taatacttag taacacatac catttatata tgagaccagg tcatgaactt
    26521 ataaaaagag ctggtggtct tcataagttt atgaattggg ataaaccaat acttacagat
    26581 agtggagggt tccaggtatt tagtctaggg cctttaagaa agataaaaga agaaggtgta
    26641 gaatttaggt cacacttaga tggttcaaaa cattttctaa ctccagaaaa agctatggaa
    26701 atacaaaatg cattgggttc agacataatg atggcatttg atgagtgtgc accataccca
    26761 tcagatagag aatatgtaaa aaattcttta gaaagaacaa caagatggtt aaaaagatgt
    26821 aaagatgccc ataataatac cgacaagcaa gctttatttg gtataataca aggtgggatg
    26881 tataaagact taagggaaca atcagcgaaa gaaataacta atattgattt accaggatat
    26941 gctattggtg gacttagtgt tggagaacca aaaccactta tgtatgacgt gttagagcat
    27001 acaactccac ttatgccaaa ggataaacca agatacctaa tgggagttgg tagtccagat
    27061 gacttagtag aaggtgttat aagaggagta gacatgtttg attgtgttct acctactcgt
    27121 atcgctagaa atggaactgc aatgactagc caaggtaaag tagtagttag aaatgcaact
    27181 tatgctgaag actttactcc acttgaccct gaatgtgatt gttatgcttg taaaaactat
    27241 tcaagagctt atataagaca ccttataaaa gcaaatgaaa tactgggtgc aagattaata
    27301 actactcata atcttcactt tttattaaat ttaatgaaac aaataagaca agcgataatg
    27361 gaagatagat tacttgattt tagaaatgaa ttttttgcaa aatatggata tgaaatatag
    27421 gttttgcact attaattata atcattttaa tatataatat tcaatagtac tcaaagatag
    27481 agaaagtaaa ttgttatata aacatttatt atgatttaca aaagagtata atttacaaag
    27541 gagtgaaaaa ttaatgccac agcaacaaat aattatgagt ataggtctat gggtagtagt
    27601 aatcgcaatc ttttatttcc taatgattag acctcaaaag aaaaaagaca aacaattaaa
    27661 agaaatgaga agcagcctaa gtgtaggaga caaggtcatt acaataggtg gaatagtagc
    27721 taatgtagct aaagttgaag atgatgttgt aatattagaa ttaggaccaa atagaactaa
    27781 agttccattt gaaaaatggg ctataggaac tgttaaatct aaaaaagaag aaatagaaga
    27841 agactaaatt agtaatttaa aagccttttc tcgaatatat attaatttga gaggaggttt
    27901 ttttatggga aaatctaatt atatttttaa aggtctagga tatgcatata taataacttt
    27961 ggctgtttta ttagtgtata acttattttt gacatttaca gatattggtg gagataatat
    28021 aactatggtc tcatcattta taactactat ttctgcagct ataggagggt tttacacttc
    28081 aaaacacatg aaagaaaaag gacttatgta tgggctttta gtaggacttt tgtacatagt
    28141 atgtattttt ttgactgtgt ttttagcaca agaaaagttt gtatttgaaa tagggatgat
    28201 ttataagtta ttgctaatat cagcagcagg aggcattgga ggcgtattgg gtgtcaactt
    28261 taaatagagt ttatattaaa gtagttttat gatataatca ataggaaaca attagaaatg
    28321 gaggcgttga aaatggaaaa taaacatgta aaaactttat ctcaagctac tttaaagcaa
    28381 agtgctgcaa aaggtggatg tggagagtgt caaacttctt gtcaatcagc ttgtaaaact
    28441 tcttgtacag ttgcaaatca agagtgtgaa agatagaaaa ttattgattt agttgtttgt
    28501 aagagggtat ctcaaagtga ttcttttatc attatgaggt atcctctttt attttcaatt
    28561 attatataaa aatatatatt ctatttatgt agaagtaaat ttgatttctt atcgtttaat
    28621 tatgcaaaaa tatctaatca tgtaagagca tttattttaa agtagctact cttatatttt
    28681 attttttatc aattacatgt attagaataa tttttaattt taaactctaa tttatg
//
LOCUS       DAMKMI010000037        27837 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMKMI010000037 DAMKMI010000000
VERSION     DAMKMI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.038"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..251)
                     /locus_tag="PC429_001529"
     CDS             complement(<1..251)
                     /locus_tag="PC429_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3847042.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNS"
     gene            436..780
                     /locus_tag="PC429_001530"
     CDS             436..780
                     /locus_tag="PC429_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF3847043.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVASYYIIKNNLNNTSIRFDVIEVYL
                     LEKRINHIMNAF"
     gene            811..2412
                     /locus_tag="PC429_001531"
     CDS             811..2412
                     /locus_tag="PC429_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF3847044.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2431..3531
                     /gene="dprA"
                     /locus_tag="PC429_001532"
     CDS             2431..3531
                     /gene="dprA"
                     /locus_tag="PC429_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF3847045.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3564..5651
                     /gene="topA"
                     /locus_tag="PC429_001533"
     CDS             3564..5651
                     /gene="topA"
                     /locus_tag="PC429_001533"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF3847046.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5816..6601
                     /gene="codY"
                     /locus_tag="PC429_001534"
     CDS             5816..6601
                     /gene="codY"
                     /locus_tag="PC429_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF3847047.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6708..7973
                     /locus_tag="PC429_001535"
     CDS             6708..7973
                     /locus_tag="PC429_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF3847048.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDIEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            8004..8774
                     /locus_tag="PC429_001536"
     CDS             8004..8774
                     /locus_tag="PC429_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3847049.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8883..9329
                     /locus_tag="PC429_001537"
     CDS             8883..9329
                     /locus_tag="PC429_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF3847050.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9347..10540
                     /gene="nifS"
                     /locus_tag="PC429_001538"
     CDS             9347..10540
                     /gene="nifS"
                     /locus_tag="PC429_001538"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF3847051.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10594..11034
                     /gene="nifU"
                     /locus_tag="PC429_001539"
     CDS             10594..11034
                     /gene="nifU"
                     /locus_tag="PC429_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF3847052.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11323..12402
                     /gene="mnmA"
                     /locus_tag="PC429_001540"
     CDS             11323..12402
                     /gene="mnmA"
                     /locus_tag="PC429_001540"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF3847053.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12545..12783
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12851..15490
                     /gene="alaS"
                     /locus_tag="PC429_001541"
     CDS             12851..15490
                     /gene="alaS"
                     /locus_tag="PC429_001541"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF3847054.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15515..15772
                     /locus_tag="PC429_001542"
     CDS             15515..15772
                     /locus_tag="PC429_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF3847055.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15782..17953
                     /locus_tag="PC429_001543"
     CDS             15782..17953
                     /locus_tag="PC429_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847056.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18159..18584
                     /gene="ruvX"
                     /locus_tag="PC429_001544"
     CDS             18159..18584
                     /gene="ruvX"
                     /locus_tag="PC429_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF3847057.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18662..18949
                     /locus_tag="PC429_001545"
     CDS             18662..18949
                     /locus_tag="PC429_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF3847058.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19231..19689
                     /locus_tag="PC429_001546"
     CDS             19231..19689
                     /locus_tag="PC429_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF3847059.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19806..20543)
                     /locus_tag="PC429_001547"
     CDS             complement(19806..20543)
                     /locus_tag="PC429_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF3847060.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20760..22436
                     /locus_tag="PC429_001548"
     CDS             20760..22436
                     /locus_tag="PC429_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF3847061.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22642..22842
                     /locus_tag="PC429_001549"
     CDS             22642..22842
                     /locus_tag="PC429_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF3847062.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22896..24059
                     /locus_tag="PC429_001550"
     CDS             22896..24059
                     /locus_tag="PC429_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF3847063.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGTVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24270..24692)
                     /locus_tag="PC429_001551"
     CDS             complement(24270..24692)
                     /locus_tag="PC429_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF3847064.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24713..25549)
                     /locus_tag="PC429_001552"
     CDS             complement(24713..25549)
                     /locus_tag="PC429_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3847065.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25847..26443
                     /locus_tag="PC429_001553"
     CDS             25847..26443
                     /locus_tag="PC429_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF3847066.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            26473..27192
                     /locus_tag="PC429_001554"
     CDS             26473..27192
                     /locus_tag="PC429_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF3847067.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27194..27733
                     /gene="scpB"
                     /locus_tag="PC429_001555"
     CDS             27194..27733
                     /gene="scpB"
                     /locus_tag="PC429_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF3847068.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11292 a         2913 c         5000 g         8632 t
ORIGIN      
        1 atcgaattaa aagtttttgt ataattacaa agtataaaaa ttaatataag ttctaaaaag
       61 taagaaataa gtataaattt attctcattt gataaagaaa aaaatctaac ttgattattt
      121 attataatta gaaataatat tatcatagtt atgaccggat acaatgtatt ttcaacattt
      181 ttcaaatagc ttacaactaa tataaataaa gataaatatc gtataaaagc aactaatttt
      241 tctctgttca ttaaaggcac tcctccaaaa aaaataaact tttcctatga aaactagaat
      301 aatatcataa aagaataaag tcaatagaag tatttattaa tattgatgga aataaaatca
      361 atccaaaaac ttgaatagta aaattgtata ctgaccataa tttaaaagtg aaaatttaat
      421 gtattggagt gaataatgaa taataaagaa aaaggtgatt ttggagaaaa agtagcagtt
      481 aattatttgc tatcaaaagg agcaaagata ctagaaaaaa attacagatt aaagataggg
      541 gaaatagata taatagcaaa aatggaagat gaaattatat ttgtggaggt gaaatcaagg
      601 tctaacataa agtttggcta cccttgtgaa tctgtaagct tcaaaaaaag aaaaaagata
      661 attggagttg caagttatta tattataaaa aataacttaa ataacacttc cataagattt
      721 gatgttatag aggtatattt attagaaaaa agaataaatc acataatgaa tgctttttaa
      781 aaaagataat gtttttggaa aggattaaat atgttaagta ttattaattc aagcaactta
      841 gtaggaatcg acagcttttt agttaaggtt gaagtagatg taagcaatgg aatacctagt
      901 tttaatattg ttggtcttcc aggcaaagaa attaaagaag caagagaaag agtaaagtca
      961 gcaattctta atagtggata taaatttcca agcacgagaa tagttgtaaa tttatcacca
     1021 gcagatataa aaaaagaagg agctttttta gatttatcta taagtatagg ccttttaaga
     1081 gagttaataa aaaaagatga aaattacata agagaaagta tgtttatagg agaattatca
     1141 ttagatggaa aaataagaaa agtaagaggt atattgccca taattatggg tgcaaaaact
     1201 cagaacataa aacgtatttt tataccaatt gagaacataa aagaaagtct atttgtagat
     1261 gaaattgata taattcctat taaaagttta aaagaatgtg tcgatttttt aaacgaagag
     1321 ataaaagtcg ataaagtaag gataatgtct tttttggatg ataagtcaag aaaagagaac
     1381 gaagaattag aaaaagataa tagttatata gattgcaaat atactaagat taataatgaa
     1441 gaaagtaaat atgatgaaga ctttaaagat gtaaaaggaa attattttgt aaagaggagt
     1501 gcagaaattg ctgctgctgg aaatcataat atgtttatga ttggacctcc aggctcagga
     1561 aaaactatga ttgcaaaaag agtaaggaca attttaccag atataagtgt agaagaaatg
     1621 atagaagtaa gtaaggttta tagtatattg ggtatgataa atgaaactaa aggaataatt
     1681 gataaaagac cttttagagc acctcatcat acaactacaa agcaatcttt aattggagga
     1741 ggaatggatg caagacctgg agaaattgct ttagcacata gaggtatctt atttttagat
     1801 gagatagccg aatttgatag aaaaatactt gagactttaa gacaacctat agaagatgga
     1861 tatgtaaata tatccagagt aaagtacagt gcaaaatatc cttgtagagt actgttagtt
     1921 gcagctatga atccatgtcc atgtggttat tatatgtcag agacagagtg tagatgtaga
     1981 agtaacgaaa ttgatagata cataaataaa atatcaggtc cattgttaga taggtttgat
     2041 atttttgtag aagttaattc tataaaatat tcagatttta attctttaaa acaagaagaa
     2101 agctctcaaa aaataaagag aagagtagaa aatgctagaa aaatacagat aaatagattt
     2161 aagaaggata atataaaaaa taatagtgaa attaaagctt acaatttatt taaatattgt
     2221 aaactggaaa aagaagcttc aaaaacggct gagatgatat ttaataaata caacctaagc
     2281 agtagaagtt atacaaaact tttaaaaatg gcaagaacaa tagcagattt agaagaaaga
     2341 gacttgataa attcacagtg tattatagaa gctttttcat ttagaaaggc ttattatagt
     2401 tattttaaat agttttttga ggtggatttt atggaaaaaa gagatactta tttatggtta
     2461 aaatcaattg gtggaataac aacaaaaaca attgaaataa tagaaaatga aatagtaaat
     2521 attgaagata tatttgattt ttctgaaaaa gaaatatata atttaaaaaa tataagcttg
     2581 aatattagaa aaaatatagt aaaatataga ggtcacgctt atttagaaaa tataaaagaa
     2641 ctactttaca aaaaagcgat taaatatatt tgtaaatatg ataaagaata tcctgaaaat
     2701 ttaaagaata tatacaacgc tccaaagtta ttattctaca aaggtgacat aggcttagtt
     2761 aataataatt ttaatatagc tatagtaggt tcaagaaaac ctacagccta tggaattaat
     2821 tgtgcaaaaa caataagttg tcaactatcc caatatggag ttaatatagt aagtggatta
     2881 gctataggaa tagatgcata ctcgcacata ggttgtatga gtggaaaatc taaaactata
     2941 gcagtacttg gttcaggagt agacaatcct cttccaaaac aaaatttaca tttatcaaat
     3001 aaaatattag aaaatggggg attattatta tcagagtata atattaactc gacagtagcc
     3061 ccgtaccatt tttctaatag aaatagaatt atcagtggtt taagtgatgg agtagttgtt
     3121 gtagaagcag caataaaaag tggtgcacta atcacagtag attttgctct tgaacatgga
     3181 aaaaatgttt ttgcaatacc tggtaatata aattctcaaa tgagtagagg ttgtcataaa
     3241 attataaaag aaggtgcaaa attaattgaa aatatagatg atattttaaa tgaatataac
     3301 atatttaata ttattgataa aaaaataaat caaaaatatg ataatataag tctaaatgcc
     3361 aagtctaagc aaataattga agctataaaa agagaaggaa acttgcatat agatagtatt
     3421 tgtgattata ctggtataga aataaagtat gtaaattcaa taatcaatga attagtacta
     3481 aatgagttag tagtagaaat gaataataaa acgtacagtt taaatgtata aattaagatt
     3541 aggtagtggg ggtgtatggt tttatggcca aaacattagt catcgtagaa tcgcctgcaa
     3601 aagctaaaac tatagaaaaa tttttaggga aaagtcatta cacagttaaa gcatcagttg
     3661 gtcatgtcag agatttacct aaaagtaaat taggtgtaga tatagaaaat aattttgaac
     3721 ctcaatatat aaatataaga ggaaaaggcg atgtaataaa agaattaaaa aaagaagcta
     3781 aaaaatctaa acaagtatat ctagcaactg accctgatag agaaggtgaa gctatatcat
     3841 ggcatttagc tcatatacta aatttagatg aatctgagga ttgtagaata gagtttaatg
     3901 agataactaa ggatgcaatt aaaaaagcaa ttaaacatcc tagaaatatt gatataaatt
     3961 tggttgatgc acaacaggca agaagagtgc tagacagact tttaggttat caaataagtc
     4021 ctatattatg gcaaaaggtt agaaaaggtc ttagtgcagg tagagtgcaa tctgttacaa
     4081 caaagttaat ttgtgacaga gaaaaagaaa taaaggcatt tattccaaaa gaatattgga
     4141 caatagatgt tgaggctaag acagtaaaag acaaagatat aactttgaaa ttttatggta
     4201 aaaatgatga aaaaattgaa ttagaaaatg aagaagctgt aaatgaaata ctaaaagaaa
     4261 tggaaggtaa agatttaaag gttgagaaga tagaatctaa atcaagacgt aaatcagctc
     4321 caaaaccatt tacaacaagt atgcttcaac aagaaggagc aaataagtta ttttttacta
     4381 ctaaaaaaac aatgatgata gcacaagaat tatatgaagg tatcgacata gaaggtgaag
     4441 gtactgttgg tcttatatca tatataagaa ctgactctaa aagaatatct gatgaagcaa
     4501 aagaaaaagc taagggctat atcgtaaaca atttaggaga aaactactat aaaaacactg
     4561 gtgataaaaa agaaaaaaag gatactaaaa aggttcaaga tgcccatgag gcaataagac
     4621 caacctcagt tgatagaact cctgagagta taaaagcctc attgagcaaa gaccagtaca
     4681 agttatacaa tttaatttgg agaaggtttg tagctagtca aatggaagat tcagtgtttg
     4741 atatactaaa tgtagaatgt aaaataggcg atattgtatt taaggcaact ggttcaaaaa
     4801 tgaaatttga tggttatacc aaggtatata atttttcaga aagagaagac aaaattttac
     4861 caagtataga aaaaggagat atattaaata tagaaaatat attgccagac caacatttta
     4921 cacaaccacc agcaagatat acagaagcta gtttagtaaa aacactagaa gaactaggta
     4981 taggtagacc aagtacgtat gctccaacaa tagctactat actgaataga gagtatgttg
     5041 aaaagcaagg cacaagtcta tgtccaacag aacttggaat aatagttact gatattttgg
     5101 aaaataattt ccataaattt atagatgtag attttactgc tgatatggaa agtaaacttg
     5161 atgttataga agaaggcaat acagattgga aaaagattgt atcagaatca tatgcaccac
     5221 ttaaagaggc tattgaagaa gctcttgaaa atatagaaaa agtaaatatg gatgaagaaa
     5281 ctgatgagat ttgcgagaac tgtggttcta atatggttat caaatatggt agatttggaa
     5341 agtttatggc ttgtaaaaac tatcccgatt gtaaaacaac tagaccaatt atgaataaaa
     5401 ttggtgttaa atgtccaaaa tgtgaagaag gagatattat acttagaaaa tctaaaagag
     5461 gtaaagcttt ttatgggtgt tctaattatc caaactgtga ctttgtagct tggaataagc
     5521 caacaggaga gttttgtgag aagtgtggtt catatatggt tgaaaaagtg actaaatctg
     5581 aaacaaaaac agtgtgttct aataaagaat gtaataaaga gacaaaagag cataatgacg
     5641 gaaaagagtg aaaattgaaa aaaatcacat aattctcttg aaaaaggttc caatctatga
     5701 taatctttca agtgtaaaca actaatagaa aaataaattt tcattaaatg tgaaaataat
     5761 attagaaaat ataaaaggtt attaaaaggc ctaaatttca tgaggagatg attaaatggc
     5821 aagtgaagtg ttacaaaaaa caaggaaaat aaataaaaca ttacaaacaa gtggtggaag
     5881 cagtgtctct tttgatttac tggccggagc attgggcgac gttttaagtt ctaatgttta
     5941 tgtagtaagt gcaaaaggta aagtactagg tcttcattta aatgatgttc aagacagttc
     6001 agttatagaa gatgagtata ctaagcaaaa gaaattttca gatgaatata ctcaaaatgt
     6061 gttaaaaatt gatgaaacat tagaaaattt aaatggtgag aagatattag aaatctttcc
     6121 tgaagaacat ggaagattac aaaaatatac tacagtagtt ccaatattag gaagcggtca
     6181 aagattagga acattggtac tttcaagata ttcaaattca ttcaatgatg atgatttagt
     6241 aatagctgaa tacagtgcaa ctgttgttgg tcttgaaata ttaagagcaa taggtgaaga
     6301 attagaagaa gaaatgagaa agaaagctgt agttcaaatg gcaataggca ctctgtccta
     6361 ctccgagctt gaagcagttg aacatatttt tgctgaattg gatggaaaag aaggtctact
     6421 tgtagcaagt aagatagctg atagagttgg tataactagg tctgtaatag taaatgcact
     6481 tagaaaattt gagagtgcag gtgtgataga atcaagatca ttaggtatga aaggtactca
     6541 tataagaata cttaatgaca aacttacaga tgaattaaaa aaattaaaaa acaatcaata
     6601 atctatattt tataggttta gattagaaaa ggtatctatt tcaaatagat acctttttta
     6661 gacgaattat taaggtaatg ataagtatat taaaaaggag tgagaaaatg cctttagcag
     6721 ataaaataag accaaaaaca atgaatgatg taataggtca aagtcatata ataggtaatg
     6781 gaaaaatatt gtctaagata ttacaatcta attttttacc aaatatgata ttttttggtc
     6841 ctcctggtgt tggtaaaact acagtagcgg aaataatagc agaaagaagc aataaaagct
     6901 tctataaaat aaatgctact aattcatctt tagaagatat aaaaaaggtg atatcagagc
     6961 ttggaagtat aaataatgta aatggagtcc tattatatat agatgaaata caaagtttta
     7021 ataaaaaaca gcaacaatca atattagaat ttatggaaaa tgggagtata actttgattg
     7081 ctagcactac agaaaatcca tatcattatg tatataaagc tcttttaagt aggtctactg
     7141 tatttgaatt taaaccttta gaaaaacttg atatagaaaa aggactgaaa agagctgttg
     7201 aagtattaaa tgaagatagc tatatggata ttgagtgcaa taataatgct attgaagata
     7261 tagcaattct ttctgatgga gatatgagaa gagccttaaa tattttggaa gtggtagtct
     7321 attcaactaa accaaataag gataatataa cttatataga ttcagatgta ataagggcat
     7381 ctacatttaa taaaattata aactatgaca aaaatggtga tagtcattat gatattttaa
     7441 gtgcatttca aaaatctata agaggaagtg acccacaggc atctatacac tatctagcaa
     7501 gacttataaa aggtggggat ttgataagta tatgtaggag acttttagta atagctagtg
     7561 aagatatagg tttagcttat ccaaatgcta ttgtaattgt aaaggcatgt gtagactcag
     7621 ctatgcaact tggatttcca gaagcgaaaa taccattagc agaagctact attcttttgg
     7681 ctacatctcc aaagtctaat tctgcatgta tagcaataat gaaagctatg gatgaactgg
     7741 ataaagagtt tgttaaggat ataccaaata gtataaagga tgctcactat tctggttcta
     7801 aagaaatagg taggggttgt ggatacaaat atcctcataa ttttaaaaat cattatgtaa
     7861 agcaacagta tttaccagat agcataaaag atagtattta ttacatacca caagaaaata
     7921 aaactgagaa aaatataaaa aaatatttag aatatctaaa tagtgatttt tagactgaac
     7981 tttatgtatc gggggtagat attatgatta taaaacttaa ctcaacttat cacagtagag
     8041 ttatgaaata cttaaaaaaa gaacctgagt acaatttgtt tataataggg gatattgaaa
     8101 gatatggata tggaaataac tttttaaata tatgggcaga tgtgggtgaa catggtgaaa
     8161 taaaggcaat attacttaaa tattttgaat ttatgatgtt ttattctgat ggagagtatg
     8221 atgttgaagg tttttataat ctacttcgta atactaatta cgaagaaata tctgggaaaa
     8281 tatgtgctgt agatgcttta gcaaagagat taggacttaa taatttaaag gtagttgatt
     8341 tttgcaaact tcaaactaaa aagtttttga tagataataa ttgtaatgca aaggttaaga
     8401 gaattaggct tggaaatcta aaaaagacag taaaacttta tgacttaata gatgaatttc
     8461 acagtacaac tttagagaat ttaaaaaatg gactaaggac aggtagagga tattgtatag
     8521 agattaacaa acaggtggtg tcaatggcaa agagtacttc agaaaatagg actcatgcaa
     8581 tgataattgg agtaggaaca catcctaaat atagagctaa aggacttgct acaaaatgcc
     8641 taattaaact atgttcagag ttactgagag aaaacaaaat tccttgtttg ttttatgata
     8701 atgaagaggc tggaaagata tataaaaaac taggatttga aaacattgga aaatggggaa
     8761 tttattcaaa ataaaaaatt aaaattaatc ttgacaaaat tagtagggta taataaaatt
     8821 atcttgaaag taattcctac taaaacagta tgaattagat ttctatatag aggtgataat
     8881 atatgaagtt gtctactaaa ggtagatatg gactaaaagc aatgtttgaa ttagcattaa
     8941 atcaagacaa tggaccagta tcattaaaat ttattgctaa gaagcaaaaa atatcagatc
     9001 agtacttaga acaaatattt tcatctttaa aaaaatcggg tctagtaaaa agtgtgagag
     9061 gtgctcaagg aggttatttg ctttcaaaga atgctgaaga cataacagta ggagatatac
     9121 ttgtagtatt agaaggacca gttgcattat ctgactgtgt actagatgaa gatgtatgtg
     9181 aaaactcaaa catgtgtgta acaaaaatag tttgggaaaa gatgaaaaag ggtatagaag
     9241 atgttataga ttcaattact cttaaagata tgataaatga ctataacaaa aataaattag
     9301 aaaatgatat aacaaatatt aaaaaataga tttggagatg aatgtaatgg aaaaaagaag
     9361 attgtatatg gattattctg caacaacacc tattaaaaaa gaagtattgg atgcaatgat
     9421 gccataccta acagattatt ttggtaatgc atctagtttt cacacattcg gaagagaagc
     9481 aaaagatgct ttggataaag caagagaaca agtagcagcc cttataaatg ctgaacctag
     9541 tgaaatatat tttacagcag gtggttcaga aagtgataac tggacactag agggcgtagc
     9601 ttatgctaat aaaaacaaag gtaatcatat aataacttca aaaatagaac accatgcaat
     9661 acttcataca tgtgaatact tagcaaaaca tcatggattt gaaataactt atttagatgt
     9721 tgatagcgaa ggaaaagtag acttaaagca actagaagat tcaataaaag atactactat
     9781 acttataagt ataatgtttg caaataatga aataggaact atacagccta ttaaagaaat
     9841 atccgaaata gctaaaaaac ataagatatt attccacact gatgcagtac aagctacagg
     9901 taatattcca gtagatgtta aagaattagg aatagatttg atgagtatgt cttcacataa
     9961 aatatatgga ccaaaaggtg taggagcttt atacataaga aaaggtgtaa gacttcataa
    10021 ctttgtccat ggtggagcac aagaaaaaag caaaagagct ggtacagaaa atatacctgc
    10081 aatagttgga tatggaaaag cagcagaatt agctaaggaa aatatgcaaa atcatgtaga
    10141 aactttaact cgtcttagaa ataagttaat agatggtgtg ttagaaagaa taccttacac
    10201 aagagtaaat ggtagcttag aaaatagatt gccaggaaat gcaaactttg cattccaatt
    10261 tatagaaggt gaaggaatac ttttattatt agatatgtta ggaattgctg gttcaagtgg
    10321 ttctgcatgt acttctggtt cattagaccc ttcacatgta cttcttgcaa taggtttacc
    10381 acatgaaata gcacatggtt cattaagact tacagttgga gattttacaa cagatgatga
    10441 tatagattat atactagaga acttaccaaa agtaatagaa agacttagaa gcatgtcacc
    10501 actttatgat gatgcgaaga aacaaggttt agtaaaatag acataaagat aaagcataaa
    10561 aaatagacat aaattgagta ggaggaaaag ataatgcaat atagtgataa agttatggaa
    10621 cattttatga atccaagaaa tatgggagaa attgataatg caagtggtgt aggtgaagtt
    10681 ggaaacccta catgtggaga tataatgaag atatttttag acatagatgg agatgtaata
    10741 aaggatgtta agtttaaaac atttggctgt ggttcagcta tagcaagttc ttctatggca
    10801 actgaaatga taaaaggaaa aactataaaa gatgcattag aacttacaaa taaagctgtt
    10861 gcagaagctt tggatggttt accaccagta aagatgcatt gttcagtttt agctgaacaa
    10921 gctgtaaaag cagctcttat agattatgct caaaagaaca atatccatat accagaattg
    10981 gatggatatg ttatagatga tgctcatgac catgatgttg aagaagaaga ataaaaataa
    11041 gtgattgtgt tatgagaagt ttacaatgat taaaaagaag tactaatgat ataattagga
    11101 cataaaaaat agctgttttt aaaatggcta ttttttattg cctaaattaa gtattttaac
    11161 ttgatttaga ggttaattag ttaacaaaat aatccataag attcaaataa taaagatgat
    11221 tattttttta gaaaatagaa caagctatat tttaaataag tacaatactt gctctatatg
    11281 gtataataat aagtaatgca aaagatgagg tgaaatttgt ttatgaataa aaaagtaatg
    11341 ataggtatga gtggaggagt tgatagctct gtagctgcat atcttttaaa acaacaagga
    11401 tatgatgtca ttggtgttac tatgaagttg tggcaagatg atgatgttgt tgaaattgaa
    11461 ggtggttgtt gctctttatc tgctgttgaa gatgctagaa gggtagcaaa taaaataggt
    11521 ataccatttt atgttttaaa ttttagagaa gtgtttaaag aaaaagtaat agattatttt
    11581 atagatgaat atttggaagg aaaaactcct aatccctgta tagcttgtaa taaacatata
    11641 aaatttgatg atttttataa gaaggctaga caaataggtt gtgattatgt agctacaggt
    11701 cactatgcaa agatagaaaa ggatgaaagt acaggaagat atttgctaaa aaaatctgtt
    11761 acagataaaa aagaccaaac ttatgcactt tataatctta cacaagagca actagagcat
    11821 actttattgc caataggtga ctatgaaaaa gatagagtaa gagaaattgc taaggaaatg
    11881 ggtatggcag tacacaataa gcctgatagt caagaaattt gttttgttaa agataatgac
    11941 tatgcaaatt atgtaaaaaa acattctaaa aagcgtattg aagaaggttt ttttgtagat
    12001 actaaaggca atatacttgg aaaacataag ggtatattat attatactat aggccaaaga
    12061 aaagggcttg gaattacttt tggaaaacca atgtttgtaa tagacataaa tccaataaat
    12121 aataccatag ttcttggaga caatgaagat ttatttaaaa aggaacttat tgctaaagat
    12181 gttaacttta tatcaataga tacgttagaa gaacccttaa gagttcaagc caaaataaga
    12241 tattcagcaa aaccatcacc agctactatt catagagtag gagaagatac tattaaaata
    12301 gtttttgatg aagctcaaag agctattact aaaggtcaat ctgtagtaat gtatgatgga
    12361 gatattgtag taggtggagg tataatcgag aaaagtttat aaaaacatct aaaaaattaa
    12421 aaaaataaac aataaagtat attaagagta agtaaagttt ataaaataaa tttattgtat
    12481 atgaaaatat agtattgatt aattttgtaa aatttagtaa tataattagt atattaatta
    12541 aaaaacaaat acagtgataa ggaatagtaa aatatttgat gtcttacaga gagagggaaa
    12601 tgctggaagc ccttagaata tgatatttga agcagccttt tagtagcaat cttgaacaat
    12661 ttatctttta taaagaagat ttaagtagag attgacggta gttacgttat agctaaggtt
    12721 ttccgaagat atcatacttt gtatgataat tagggtggta ccgcgattgt cctcgcccct
    12781 ataaatttat ttatagggac gaggtttttt atttaaacga aaaaataaaa tttattagga
    12841 ggaacatgat atggaaaaga tgggattaaa tgaaataaga agtaagtttt tagaattttt
    12901 tgagtcaaaa ggtcattatg tagcaaatag ttattcttta gtaccaaaca atgataagag
    12961 tttgttactt ataaactcag gtatggcacc tttgaaaaac tacttttcag gtgtagaagt
    13021 acctccaagt gtgagaatgt gtacatctca aaaatgtata agaacaggag atatagaaaa
    13081 tgtaggtata actgcaagac atgctacatt ctttgaaatg atgggtaatt tctcatttgg
    13141 agattacttt aaaagagagt caatcaaatg gggatgggaa tttgttacag aatggctaaa
    13201 tataccagaa gataagatat gggtgactgt atatgaagaa gatgatgatt cttatgatat
    13261 atgggcaaaa gaaatgaatt tcccagaaga aagaatggtt agacttggaa aagatgataa
    13321 tttctgggaa ataggtacag gtccttgtgg tccttgttca gaaatatatt ttgatagagg
    13381 agaagaatat ggatgtgaca atccagactg taaaccaggc tgtgattgtg ataggtattt
    13441 agaattttgg aaccatgtat ttactcaatt tgatagagat gaagaaggaa attatagtct
    13501 attagaaaac aagaatatag atacaggtat gggtcttgaa agaatgggat gtattatgca
    13561 aggcgtagac actatctttg aagttgatac aattaaatct atactagaag cagttgagaa
    13621 gttaacagga gtaaaatatg gagagaatcc aaaaaatgat atatcaataa gaataataac
    13681 tgaccacata agagctgtaa cattcttagt tagtgatgga gtgcttccat caaatgaagg
    13741 aagaggatat gttcttagaa gactccttag acgtgctgct cgtcatggta agttattagg
    13801 agtaaaagaa ttatttttac agaaactaat agatgaagtt ataaaagtaa atgataaagc
    13861 atatccagtt ttagttgaaa aagaaagtta tattaaaaaa gtagttggaa tagaagaaga
    13921 aaaattcaat gaaactatag accaaggaac agaaatattg aactcttata tagaagtatt
    13981 aaaaaatgaa ggaaagactg tcctaagtgg tcaagaagct tttaaactat atgatactta
    14041 tggcttccct atagacttaa ctaaagaaat attagaagaa gaacatctat ctgtagatga
    14101 agaagctttt aatgaagaaa tggaaaagca aaaagaaaga gctagaaatg ctagaggaaa
    14161 tatggatgga gaaagttgga aggaagaccc tctatcaaaa ttagaatcta cagttgacag
    14221 tacatttaat gggtatagtg aaatttatgg tgaaggaact attgaagcta tagttaaaga
    14281 tgatgagcta gttcaaagtg cagaagaagg cgataaggta tctatagtac tagataacac
    14341 tactttctat cctgaaggtg gaggacaagt tggagattgt ggattgataa ctaatgaaaa
    14401 cttagtttta gaagtattaa atactaaaaa aggtgctaat aatagtataa aacatatagg
    14461 tataataaaa tcaggaagaa taagtaatgg ggacaaagta aagacactcg tagatagaga
    14521 aactagaatg tcagctgcaa gaaatcacag tgctacacat ttacttcata aggcattaag
    14581 agaagtatta ggagaacatg ttaatcaagc tggttcttta gtaactccag aaagacttag
    14641 atttgatatt actcactttg aagcgatatc aaatgaagaa ttaaaagtaa tagaagaaaa
    14701 agtaaataat gttattttat catctttaga cataaaatgt gatattatga atataaagga
    14761 agctaaagaa aaaggagcta cagcactatt tggtgaaaaa tatggtgatg aggtaagagt
    14821 agtttctatg ggagattatt ctacagaact ttgtggagga actcacttga caaatacttc
    14881 tcaagtagga atgttcaaaa tattatctga aggtggagta gcagcaggtg taagaagaat
    14941 agaagcaata acaggaaaag ctgtttatga atacctaaaa gaaagagatg gaattatctc
    15001 tgaagtttgt gtaaacttaa aatctaaaga agataattta attcaaagaa taagttcttt
    15061 attagaagaa aataaaaact tatctaaaga attgcatgat atgaaagcta agatgagttt
    15121 acaatcagtt gattctattt ttgattctaa agttgaagta aatggagtta atttgataac
    15181 taataaattt gaaggtatgg atatggatac actaagagaa acagcagata atctaagaga
    15241 taagcttggt tctggtgtag tagtacttgc aaatgtagta gatgataaag ttaactttgt
    15301 agttactgcc actaaagatg tattagataa gggaatacat tcaggtaata ttgttagaga
    15361 agttgcaaaa atagctggag gaaaaggtgg aggaagacct aatatggcac aagcaggagc
    15421 tagtgatgta tctaaggttg accaagcttt aagctatgca agtgaggtta ttaagacaca
    15481 agttaaataa cattcactca aggaggtttt tagtttggaa aaagatttag actacactat
    15541 gaaatttgaa ggaataccag aagataggat gagtgtagga gatactattg attttgtata
    15601 taaagcatta gttgaaaaag gatataaccc tataaatcaa attatagggt atctcctttc
    15661 tggtgattct agctatataa ctagtcataa aaatgcaaga gctataatta aaaaatttga
    15721 aagagatgag atacttgaag aagtaatcac gcactatctg aatagaaagt aggtcaaata
    15781 aatgagaaaa aagattatgt cgttgtttgc aatagtcatt atgatttttg tatcaactgc
    15841 tagctatggt tttgctgaag aaaaaggcaa ggtcatattt attgatatga ataggaccaa
    15901 tcttggaagt atgatgaata ttccaatctt gaaaaatgag atagaaaaga gaggctatgt
    15961 agctctcatg aatataagag gggatcaagg tactgatgat agaaggtctt atgcaagtat
    16021 gggtgcagga acaagagcta gtgtaactac tgaagactat atcaattttc aaaaagtaga
    16081 ccaagatact gctaaagctt ttgaagtcgc aacaggtcaa aaagctaaag aaataaatga
    16141 tttaggcatt aatagatcta taaatgaaaa tcttgagtat ggagaatatg gagcaaattt
    16201 agggtcatta ggaaaaactt tatcagaaaa tggtttaaag acatctgtaa ttggaaatgc
    16261 tgatataatt gaaaataatc aattagttaa aaacagaaac ttatgtttag tagctatgga
    16321 ccaatatgga aggattgatt caggtaatat tgataatatt aataagaaag attctaaaat
    16381 gccatttgga atatctacag attatgataa attactagat gaaactaaaa aatcctataa
    16441 aaatagtgat gctatatttg tagaacttgg agatacatat agattagata tgtatagatt
    16501 aaatctaaat gataatactt atgatacaat gaagaaaaat attgtaaaaa atataaataa
    16561 atatcttgaa caagtatttt ctatggttgg agaaaatgac actgtttata tagcaagtgc
    16621 atttccaagt gacctagatt ataagaataa gagaagactt tctccaataa taaaatttag
    16681 tggagaaggg aaaggtctac tggaatctgc cacaacaaga aaggctggaa ttgttgccaa
    16741 tttagatgtt ggagtagata tactaggtac atttggtctt gaaaataaag agatggtagg
    16801 tagaagttat actttagtaa ataaagagga caatattagc tatctaaaat cactttatga
    16861 aaggatagtg tctatatctt caataaggtc aactattata aatggctttg taggcgtaat
    16921 atctgcttct tgggtaattg ccatgtttgc gattttcttt agaaattata tgccacataa
    16981 agagcgtgta ttcaaagtac ttaaggagtt tataaaatta ggcataataa tgccactatc
    17041 atttatgtta gctcctttga tgaattttaa aactccaatt tcaatatctt tgggaatatt
    17101 tgcgactacg ctaatattat acttgttagg tagaaaatta tttaaggaca atgatataaa
    17161 gcacatggga ttttttgcaa cacttactat tttagtcata gttatagatt ctgtatttgg
    17221 aacatactta atgcaaaata acataatgag ttatgatgct gtcataggag ctaggtacta
    17281 tggtgtagga aatgaatatg aaggtgtaac tatagcaagt gcagtatttg gcttagcagt
    17341 attattacat tataagaagg tatctaaatt acttgttgta attttttcat taataatact
    17401 tataactagt gcatatcctt caatgggtgc aaatgttggt ggagctatat cagaatgtgt
    17461 agcatatttg ttgtttataa tgcttatata tgacattaaa atagatttta agaaagctgt
    17521 attattagct gtttcagctg tagttgtcgt ttttgcattt gcagctttag atatactttc
    17581 aggtagtgaa tctcatttag gtatgtttgt acagagaatt ttcctaaatg gaccaggaga
    17641 gatattacaa acatttggaa gaaaaattca aatgaatatg caactagcac agacaagtgt
    17701 atgggtaaat attttacttg taggaattgg agtaattgca gtgcttatat ttaaaccatc
    17761 tagacacttt agaaagataa tgaataattt tccaatcttg tttaaagggt ttattgcatc
    17821 tatggttggt tgtataatta ctcttttagt taatgattct ggaatagttg ctgcatctac
    17881 agcatctata tatatactta taccactaat aataataagt ataaatatga taatatttga
    17941 aaaaaataac taaaaaaatt tttactgttc atataggatt taaataaaaa atgcaatatg
    18001 attattaatg taaactggat gcttacataa aaaatcatat tgcaattttt atgattgatt
    18061 atgtaaaatt atatgaagca gtaagcctgt gaccttagtt atgataggtt cagaatatgt
    18121 atttaatcta tagaatataa atagataaag gaggaactat gttagatgga agaataatgg
    18181 gtcttgatgt tggtgataaa actattggag ttgctgtgag tgatttgatg ggattaactg
    18241 cacaaggtgt aaaaacaata aaaagagttg gcaagaaaaa agatattgaa gagctaaaag
    18301 caataataaa agaaaaacaa gtaaataaaa ttgtatcagg attaccaaaa aatatgaatg
    18361 gaactttagg tccacaagga gaaaaagtaa tcaagttttg tgaattggta aaagcagaaa
    18421 caggtattga tgtagagttt tgggatgaga ggctttctac agtagcggca gagaggtctt
    18481 tattagaagc agatgttagt agacaaaaaa gaaaaaaggt tatagatatg ttggcagcag
    18541 taataatttt gcaaggatat ttagatttta aaataaattc ataaaagaac tataaaattg
    18601 aatacttatg atatataata taaataataa aaataaaaat aaatttgagg tgaatgacat
    18661 tatggaagaa aatataataa atttaataga cgaaaatggc gttgaaagcc aatttgaaat
    18721 aatattaaca ttagaagcag aaggaaaaga gtatgcaata ttaatgcctt tagatgatga
    18781 agaagctgaa gaagctttaa tctttagaat tgatgaggat gaagaaggtg aaatcttaat
    18841 acctttggaa agtgatgaag agtatgaaac tgttgtagct gtgtatactg ctataatgga
    18901 agaagaaggt ttaaattacg acgaagatga atcaaatgga cttaactaga taagtaatta
    18961 agtttgtata taaaaatcag atatttatta tatttgcttt aattaaacct tattaattgt
    19021 aaaagcattt atagtatata tctgtttttt tgttttttat taaaaatacc aaaaaattta
    19081 tgaattttgg tgaataatat atcttttatg ggtatatatt tatattatga gaataaatta
    19141 aatttattta aatgtcttga caaaaaatga attatcaatt acaatattat ttaataaaga
    19201 ttatcaattg aaaatagtaa gggtgatgtt atggcaaata caatggattt attaaaagac
    19261 aagttaaaag aaactggttt taagattacg ccacaaagaa gagcaatagt tgaaatattg
    19321 cttaaacatg accattcaca tctaagtagt gaggaaatct atgatttagt tagggttgac
    19381 tgtcctgaaa taggcttagc tactgtatat agaacaatgc agctattgga tgaaatagga
    19441 ttaatatcaa aactaaactt agacgatgga tgtataagat atgagattag tctacataaa
    19501 gaagactgtc ataatcatca tcatcttatt tgtaaaaatt gtggaaagat aatggaagct
    19561 aaagaagacc ttttagacaa tatagaaaaa gagatacaat cattgtacaa atttaaaata
    19621 cttgatcatg atgttaaatt ttatggacta tgtgacgagt gtaatggagt aagcgattca
    19681 gaagaataaa cttctattgc ttaatgtcga tggaagaata gtatagaact tttatattct
    19741 atgctattct tccatttttt attataaacc atctataaaa tgcaaatata cagttttcaa
    19801 gatgcttata gttttaatat tgaaaatata attaatccta taagtacaaa aatgaatgat
    19861 ttttctatga aattactaaa tggtcctcca acagttaaaa aacctaaaga aattttcttt
    19921 tcgcttagcg gataaaaaat tggtattcct tgctttgtaa ataaatctcc taaaaacata
    19981 tgacttgcat aaccaatcgt accataaaaa gataaattat taatattagt gattaattct
    20041 atctgtttta gtaaaaaata tactattata gttgcaaata tgctatgaga aaaacttctg
    20101 tgctttaacc agggaaaaat tgacaacatt agacaaaaaa ttgtaaaata tatttcaact
    20161 tctatgatgt ataagcatat agctaatagt ataaatatta atgatagaaa aacttttctt
    20221 agaaatgtgt gggttagttt tgcttcaata attataattg caacaaatgt aattattgcg
    20281 cttaaataaa aattatcatt tatatttatt gaaataaaga aaataataat attaacagca
    20341 tatataaaaa ttctatatat gtatttggat acattgtttt ttagaatgaa atttgagacc
    20401 atagaattag atttatccaa atcgggtagt acagcaaaag tagctgaaac taaaatatca
    20461 actaaagata taggaatttt aaatattaaa gatgtttgga tagctgttaa gattcctatt
    20521 gcacaatgag ttttaccacg cataaatcct cctaaattat ccgatattaa atcatataat
    20581 atttagagga tatcaaaata aaataggttt gtcaaaataa tacgttaaat gtattttaaa
    20641 tatagcttaa aatatgatac gaattatggt acaataagaa taactggtag cgagtgcgtg
    20701 atttgatatt atttatgatt gtagaacgtg ttaaaaaatt aaatagacag aaggagatga
    20761 tgcaattgtt caaaaagaac accaacaaga taaaggtaat ggccttgggt ggacttaacg
    20821 aagttggaaa aaatatgact gttgttgagt ataaagatga aataattgtt atagatgctg
    20881 gacttagttt tccagaggat gaaatgttag gagtagatat agttatacca gatataactt
    20941 atttagtaaa aaatagggat aaaataaagg gtatatttat tacacatgga catgaagacc
    21001 atataggagc tcttccatat atattaaaga aaataaatgt acctgtatat ggggcaagac
    21061 ttagtatagg tcttatacaa gtaaaactta aagaacataa aatgaataat gttaagttaa
    21121 atgttatagg tcctagacaa gttataaagc ttgataatat ggaagtagag tttttgaaaa
    21181 ataaccatag tataccagat gcttactcta ttgcaataca cacagaccaa ggtataatat
    21241 accacacagg agattttaag attgacttga ctcctattga tggagatgtt atggatatgc
    21301 atagaatctg tgagttgagc aaaaaaggag ttcttttaat gctggcagat agtactaatg
    21361 cagaaaaacc aggatttact atgtctgaaa agactgttgg ggtaggtctt gacgaattat
    21421 ttgcaaaagg aaatggcaga agaattatag ttgctacctt tgcatccaac atacacagat
    21481 tacaacaaat aataaatact gctgaaaagt ttaatagaaa agttgctata tcaggtcgct
    21541 caatggtcaa tgtagttgga gttgcaaaag aattaggata cctagatatt tctgatgaca
    21601 tgcttataga tttaaatgat atatgtaagt atgaagatag tgaattagtg ataataacaa
    21661 ctggttcaca aggtgaacct atgtcagctc ttgctagaat ggctttttca gagcacaaaa
    21721 aagtagaaat aaagagtggc gatttagtta taatatctgc tcatccaata ccaggaaatg
    21781 aaaaattaat atcaagagta atcaatttct tatttgaaaa aggagcagaa gtagtataca
    21841 gtgatatagc tgatatacac gtttctggac atgcctgtca agaagaatta aaacttatac
    21901 atgcattagt tagaccaaag ttttttatgc cagctcatgg tgaatataga atgttaaaaa
    21961 gacatgctga aatagctgaa cagcttggta tggataaaga aaatattttt gtcatgcaaa
    22021 caggagatgt tttagaatta gataaaaatt ctgctaaggt agcaaaccgt atacaaacag
    22081 gaaacatatt agttgatggt ctaggagtag gcgatgtagg taatatagtt ttaagggata
    22141 gaaagcattt atcagaagat ggtcttatga tagttgtagt tactatttcc aaggatgagg
    22201 gtaaggtctt agcaggtcca gatataattt ccagaggatt cgtatatgta agagagtcag
    22261 aagatttaat ggatggagca aaggatataa ttaagaatgt tctaaatgaa tgtgaagaga
    22321 aaaatattaa agaatgggct tatttaaaga ataatattaa agaaaaccta aaagaatatt
    22381 tatatcaaaa aactaaacga aatccaatga tacttcctat cataatggaa gtataatata
    22441 aaaaaactat aaatttgaaa attagacctc ttgtaatttt taaagtaata tcaaaaatcc
    22501 aattgatatt actttaaaaa ttacaagagg tctttttaat actcattaaa aaaaaaaaaa
    22561 tattacataa aataccaatt ataacaatct tttattagta aaaactactt atatataaat
    22621 tttactgata ggaggattct tatggatgat gtttcaagac aaaatgctat aaaagcttta
    22681 aaacaaacaa aaatggaaat agcaggagag tatggaatga attatgaaga tgcttttgaa
    22741 ataatagaaa atgcgtctaa taaaggtgtt ttagaaggtt actttaaaaa gcttgaaaag
    22801 aaaaaaaatt taggtcaagg tatatctagg catttagaat aaaaaaagtt aaatgtgaga
    22861 aaataagttt aaataaactt taagaggtga catcgatgaa aaaattagct agtactgctt
    22921 tggctatatt aattgcgctt actcctttaa gcttttcttt tgcaaataat aaggaaaatg
    22981 ctgatgccaa ccaactaaat atatcttcaa aatcagcaat tttgatggat gttggtagtg
    23041 gacaaatttt atatgaaaag gatgctcata aaaagcttcc tcctgctagt gtaactaagg
    23101 taatgactat gctacttata gtagaagctt tggattcagg caagattaag cttgatgatg
    23161 aagttcaagt aagtgaaact gcatctagta tgggtggtag tcaaatattc ctagagccag
    23221 gagaaactca aaaggttgat acattattaa aaggtatagc tgttgcttct gcaaatgatg
    23281 cttgtgttgc tatggctgaa cacttagctg gaactgttga aggatttgtt gatagaatga
    23341 atgctaaagc aaaagagtta aatatgaatg atactcattt tgcaaataca aatggacttc
    23401 cagttgcaaa ccactataca tctgctcatg atatagcttt aatgtctaga gaactattaa
    23461 aacacgaaat gataagtaaa tacttaacta catggatgga taaggttgta gtagggaaga
    23521 aacaagttac agtaggtctt gcaaatacta ataaacttat aaagcattat caaggagcaa
    23581 ctggtgttaa aactggtttt actcaagaag caaaatactg tttatcagca tctgcaaaga
    23641 gaggtaatac tcatctagtt gctgcaactt taggagcaga gacttctcca gaaagattta
    23701 atgatgcctc tagcctttta aactatggat ttgctaatta tgaaagtgtt aagttatgta
    23761 gtaaaggtga taatatagca acacttacat tggataaggc tgatgaaaac aaagttaaat
    23821 tggttgctaa agaagattta aatgctctta ttaaaaaagg aagctctaaa gaatttgaga
    23881 agaaaattga aatagtaaaa aatcctaaga tgccaattaa aaagggaact gttttaggaa
    23941 aaataaagat atgtaaagat aaaaaagtaa taggcgaagt ggaattgata aatactaagg
    24001 atataaataa ggcaagctat ctacaaatgc ttcaaagaat aattgataat atgatataaa
    24061 taattatcag atgaaatata attataatac acaaagatat gattataata cacataaaaa
    24121 atacccttat gtaattattt gccatagggg tatttttatt tactatatag aagtatttta
    24181 taatcttatg atttttatag aaatatagaa aagtttatgt atcttaaaat atcatttgat
    24241 ttaatatcat atatagggtt gtttaacaat tatctaagta aaggcatact accagtaagt
    24301 ttattcactt tctttttatt cccataaatt gctatcccaa aatactgtaa ctcatcttct
    24361 ggaacactag caatcttttg tgtaaattct tcataaagat tgcaactttg tgctacatct
    24421 gaaaaatcta caacagttaa atcgttaaat tccaaagtat ataatttttt acgtaaatct
    24481 tttattattt ctttgtttcc ttttaaaata ggtacaggaa tgtcaataat tcctaagtgg
    24541 ctattacctg ttttgtcaat tacatctggc ccaacagttt ctggggcatg ttttcctagt
    24601 gtaattccca ttatagctgc tgtattggat ataattccca ttggtaaatt ttcgtctatt
    24661 accatgacac attttgaatt tttattttcc atttttattt ctccttttta ccttatttaa
    24721 agttgtttga atcatcattt ataaatatat tttgatactg ctttggtgta agaccaataa
    24781 aattcttaaa aaagtttgta aagtgacttt ggtctacaaa tcctgtttgt atcgcagcat
    24841 ctataggttc tacgcctttt tctaaaagct ttttagcttt gtttacccgt atattttcta
    24901 ggtatctata tggtgtaata ccttttaatt tcgtaaaatt acgaagtaga gagtacttat
    24961 tcatacctgc tatagtgctt aattcgtcaa gtattatatg ttcagcataa ttattttcta
    25021 gataatcaca tactgcttga atttctatat ttgtattttc caagttagat tgcaaatttg
    25081 gctctgtatg ttcttcaatt aattgttcaa ttaggaagaa aaaaagctct tcctttttaa
    25141 aatccaattc ttcttccatg attatatagt gtaattcttg tagtagaggt actaactcac
    25201 tgcgaaatat aacaggttga ttaaattttg gtaaataatt tttacctgta atttcaaaga
    25261 cggttttttt cattatttct ggcttaatat taatacatct gtaatcaagt actttatcat
    25321 ctatttgttc acaagtatga ctatcaaaag ggttaaaaag tagcaaatct ccagtacttg
    25381 tggtatactc tttattttta caagataaat atctttgacc tttttctata aaaccaatta
    25441 cataatgttc atgaaaatgg tttgggaatt tttgcattat gcctttaaat ctatatgctt
    25501 caatttttaa ttccctatca aagcatactg tgcgttcttc ttgtctcact taggtaacct
    25561 cctttcatgt tatacaaagt gtaacacata tttttttata cttcttgtat aatattgact
    25621 aagagacaat aatgtcaatt catgtcaata taatattaca cttacagtag gtgtaattat
    25681 tatattttca caatgaatat gatataataa taaaagttat aaaataaaaa gcaaatttta
    25741 attttatgcc tagaagttaa aattattata atgtaaaata attttgatta taagctattt
    25801 aattgttata ataatattaa aaatttaatt taggagatgt acattaatgt taagtatgaa
    25861 tataggagaa atagtagcaa gtttagttgg tatagctatg gcaatatcaa tacatgagtt
    25921 tggccatgct tattcagctc acttattggg agatgataca gctaaagctt atggtagaat
    25981 gacattaaat cctgtaagac atgttgaccc aataggtctt ttggctatgt ttatccttaa
    26041 gatagggtgg gcaaagccag taccagtaaa tccaaataac tttaaaaact ataagatagg
    26101 aaacctgata gtttcacttg caggcgtttt ttgtaatgtt ttgactgcta tactgtgtgt
    26161 aattatcaat aagtatgtgc atatgtatgc tataaataca atagctgaat atgtgttttt
    26221 atatagtatt ggatttgcag cttttaactt acttccgata cctccacttg atggatgggg
    26281 agttatatct acatttgtac catataaatg gaatgaatat ttgtataaat ttgaaagtat
    26341 gagttatata attttattaa ttgctttatt tactggtatg tatagaataa tattaacacc
    26401 aatatatgca gtgttgctaa aaatagtata tttatttgtt taatataata aacagattaa
    26461 agagggattg atatgaagta taatatacaa ttacaagtct atgagggacc attagattta
    26521 ctttatgatt taataacaaa gcataaaata gatataaagg atatatccat aatagatata
    26581 actaagcagt atctaaatta tttgaaaatg ctagataaaa tggatttaga gataactagt
    26641 gaatttataa ctatggcatc aaaactttta gagataaagt ctaaatatct gctttataag
    26701 caaaaagatg aagaagaaga cccaagaata gaacttatgg aaaagttaga agaatataga
    26761 aagtttaagg tagcatcaca agatataaaa gaaaatataa cttatgttaa tgagagattt
    26821 tatagaaata aagaagagat aattattgat gacaatgtgg atttagaaga tatatcaatt
    26881 gaagcaataa aaaatatact tccatacata tttaaagtta aaacatcaca gatagaaaat
    26941 gctaatgatg agaaactaga taaaatagta aggaagaaaa taatatctgt agaagaaaaa
    27001 atattatata taagagatat tataaaagag aagattgaag ttacttttac aaatattata
    27061 aaatcatatg aaaatgatga aataatagct acttttttat ctatattaga gcttataaaa
    27121 gaaaaagaaa tagtcgtagt acaagatata ttttttgatg atattctaat aagaaaaagt
    27181 tcggagtgtt aaaatgaaaa gagaagatat aaagtatatt atagaatctg ttatgtttgc
    27241 atatggtgag ccaataagta taaaagaatt aaactatata ataaataaag aactttcatc
    27301 aaaagaaata gagattatgt taaatttact tattgaggag tacagagaac aaaatagagg
    27361 tattcaaata atcaagttag agaataagta ccaaatgtgt acaaataaag actatgcaga
    27421 atatataaag aagattattg aaccaaagaa aaagaaaagt ttgagccaag ctacattaga
    27481 aactcttact ataattgcct acaaacaacc aataacaaag gtagaaatag aggatataag
    27541 aggagtaaaa tgtgataagg tattacaaac tttgtttgaa aatgaactta taagagaagc
    27601 tggtagattg aataagatag ggaaaccaat aatttataaa acaacagatg aatttttaaa
    27661 gttgttgaat atagaaagtc ttgaagaatt gccacctata gaaaattatc aagaagtggc
    27721 aacaaatgaa taaaaatata aaatcacaat aatttagtat gtaattttaa attttaaaag
    27781 atttaaaatg ctctaagttg ttgtgttttt ttgtataaat ataaaattat aattctc
//
LOCUS       DAMKMI010000038        27369 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMKMI010000038 DAMKMI010000000
VERSION     DAMKMI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.039"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..591)
                     /locus_tag="PC429_001556"
     CDS             complement(<1..591)
                     /locus_tag="PC429_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF3847069.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
     gene            complement(924..2138)
                     /gene="dpaL"
                     /locus_tag="PC429_001557"
     CDS             complement(924..2138)
                     /gene="dpaL"
                     /locus_tag="PC429_001557"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF3847070.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2752..3289)
                     /locus_tag="PC429_001558"
                     /pseudo
     CDS             complement(2752..3289)
                     /locus_tag="PC429_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(3366..5171)
                     /locus_tag="PC429_001559"
     CDS             complement(3366..5171)
                     /locus_tag="PC429_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3847071.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(5567..5944)
                     /locus_tag="PC429_001560"
     CDS             complement(5567..5944)
                     /locus_tag="PC429_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF3847072.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6849..7334
                     /locus_tag="PC429_001561"
     CDS             6849..7334
                     /locus_tag="PC429_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847073.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(7534..8073)
                     /locus_tag="PC429_001562"
     CDS             complement(7534..8073)
                     /locus_tag="PC429_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3847074.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(8066..8479)
                     /locus_tag="PC429_001563"
     CDS             complement(8066..8479)
                     /locus_tag="PC429_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3847075.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(8515..9636)
                     /locus_tag="PC429_001564"
     CDS             complement(8515..9636)
                     /locus_tag="PC429_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF3847076.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            10235..11887
                     /locus_tag="PC429_001565"
     CDS             10235..11887
                     /locus_tag="PC429_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF3847077.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(12270..13196)
                     /locus_tag="PC429_001566"
     CDS             complement(12270..13196)
                     /locus_tag="PC429_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF3847078.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
     gene            13786..14706
                     /locus_tag="PC429_001567"
     CDS             13786..14706
                     /locus_tag="PC429_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3847079.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            14792..17212
                     /locus_tag="PC429_001568"
     CDS             14792..17212
                     /locus_tag="PC429_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF3847080.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            17212..18234
                     /locus_tag="PC429_001569"
     CDS             17212..18234
                     /locus_tag="PC429_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF3847081.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            18387..19133
                     /locus_tag="PC429_001570"
     CDS             18387..19133
                     /locus_tag="PC429_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3847082.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            19219..20469
                     /gene="cme"
                     /locus_tag="PC429_001571"
     CDS             19219..20469
                     /gene="cme"
                     /locus_tag="PC429_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF3847083.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(20986..21999)
                     /locus_tag="PC429_001572"
     CDS             complement(20986..21999)
                     /locus_tag="PC429_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847084.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(22002..23030)
                     /locus_tag="PC429_001573"
     CDS             complement(22002..23030)
                     /locus_tag="PC429_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847085.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            complement(23030..23875)
                     /locus_tag="PC429_001574"
     CDS             complement(23030..23875)
                     /locus_tag="PC429_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847086.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            complement(24306..25436)
                     /locus_tag="PC429_001575"
     CDS             complement(24306..25436)
                     /locus_tag="PC429_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3847087.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            complement(25433..26104)
                     /locus_tag="PC429_001576"
     CDS             complement(25433..26104)
                     /locus_tag="PC429_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847088.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            complement(26307..26957)
                     /locus_tag="PC429_001577"
     CDS             complement(26307..26957)
                     /locus_tag="PC429_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF3847089.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
BASE COUNT         9498 a         4423 c         3299 g        10149 t
ORIGIN      
        1 acctttttct ggattagcag agtgagctgg tttaccaaaa gtttctacta ctatttctcc
       61 tctacctctt tgacctatct ttaagtttaa ttctgaagat tcacctatta caacataatc
      121 aggttgaaca gccttactta tctctctagc tgaaacacct tcaaatattt cttcatgaac
      181 aacacctgct acatacaatt caccttcaaa gtctttgtta gtatcttctg caaagtatga
      241 aacagcagcc atcatagcac ttacttgccc cttcatatca gaagttcctc taccatatat
      301 tttcccatct actattctcg cttcaaaagg aggaacactc catttacttg catctggaac
      361 tggaactgta tctatatgcc cgtcaaataa tattttttta cctggtttat ttccttttat
      421 acatcctact atatttccat atctatctct tgaccaacta tcaaaaccta gtaaagcaaa
      481 attttcttct atagctttta ctacgttttc ttcttgacct gaataactag catttcttat
      541 taagtcttgg catacttcag ttaattgttt ttttcttgtt tcatttaaca ttttgatatt
      601 cccccatata atttttagtt tgttgacgtt ttattttgat atttcacttt tttaatttat
      661 tagcatttca cttaatctat tgatttttta tttctttata ttttatttat taaattttta
      721 tttagacttt attttaattt tatttggttt tatttaattt tatttagttt ttattttgaa
      781 tttttcattt gctttacttt ttcatttatt tagatttttc atttgtttta gtttttattt
      841 tatttaaagt tttcacttat ttaagttttt attttcctta aatttttcat ttatttgtta
      901 agtttttatt ttccttaagt cttttattta ctttggcatt caccattcca aactatatct
      961 ctatatttat cagggtcagt atctccttct gtacttatta ataatacttt agagttctca
     1021 tctagtttca aagcttctct taagtctttt aaatcttcac tttcaaggat tgaaactaat
     1081 gctccagttg taactgctcc agattctcca gagattactt tctcatctcc tcttagaggg
     1141 tttcctaata ttctcatacc tttagcagat acccagtctg gtgctgaaac gaatgctgat
     1201 gaataatttt ttaacacttc aaaacctata gtgtttggtt ctccacaagc aagccctgcc
     1261 attatagttg gcatatctcc tccaacaaat cttggttttc catctccagc ttctgctgat
     1321 ttatagtaac aatcagcctc atcagcctct actactactg ttattgggca ttcatcatat
     1381 attgaagcaa catacccttg aactgcacca gcaagtgaac caactcctgc ttgaacaaat
     1441 acgtgtgttg gtctatctac tccatattct tttaattgtt ctatagcttc tttagccata
     1501 gttccatatc cttgcattat ccatgctggt atttcttcat atccgtccca tgctgtatct
     1561 tgaaccatta ctccatggtc atcagcttct gcataatcat tagctaatct tactgcatca
     1621 tcatagttta agtctgttat actagcatct gcaccttctc ctttgatttt atttaatcta
     1681 aattcagaag aaccttttgg cattaataca acagattttt gacctaattt attagctgtc
     1741 caagcaactc ctctaccgtg atttccatct gtagctgtaa agaaagttat ttctcctaat
     1801 ttttctttta tttctttcga agtcaatttg tcatacccta actcagatat atctgtattt
     1861 aatctttgag ctaagtatct acccatagag taagaaccac ctaaaacttt aaatgcgttt
     1921 aagccaaatc tataagattc gtctttaaca tatattccgc ttacgcctag tgattttgct
     1981 aagttatcta atttaactaa aggagtcttt tcatattgtg gaaagctttc atgaaaagct
     2041 ttagcctttg caatctcttc atcttttaaa aattcaattc ctttttcttt ttcctctgat
     2101 ttaggtaagt tatttaattt ccattttatt tctctcattt attttatatt cccctttcaa
     2161 atttttcggt gtcattttaa ttttttatct ttgtgcctta tagcaaagca attaccgtgc
     2221 caatattatt tattctaaaa acaatttttt tatgttggaa ttacatactt ttataaaatt
     2281 tctttaaagt ttataaattt tgagtgtgaa aaataagatt tataaaagaa aaaattctca
     2341 tattgggaaa tttttatcaa tacgagaatt atcaattcga gaaatcgttt atttatctta
     2401 attcttcctt tattatgctc ttatttttta tatgtctatt gttttgtcag atatattact
     2461 acttttattt tctataaata tatggctata accttcttac gcataaactc tataaattta
     2521 tatactttat tacccaaata ttctttatta cccaaagcct ccaaaatggc ttaaatcttt
     2581 tcatagcttc ttacagaagt ttacatggtc gtccccccaa taagcaaaag tgcaatatgt
     2641 gtaccattgt gccctacttg cgactattga gctgtcgaaa ttcgcaactt cgcatttatc
     2701 tgatgaaatg cactatacaa aatattcata acatccacaa cagagttaaa gttacctatt
     2761 catattcact gtaaaaattt cttccacagg aaatgtaatt ttaaccaatc ctattgagac
     2821 aaactcacca cttatttttt tgaactcacg cagtaataca tcacttgccc gaaaaaaagc
     2881 ctcactttca tcatctccag taagtgctat tgtgcaatgg ggtacccaac gattggggtg
     2941 gtaccattcc caaccatttg aatcaaaatc ttgtagtacc tcatataatt ctctctgaaa
     3001 ctgatacatt gctattcatt ataggtgata caaacaccgt tttagtatca ttaaacatgc
     3061 ctactgaacc aatgtaagca ggtattacct tatggttttg tgcaaacccc tttagcttgt
     3121 ctatgcaaca agcctcatcc acatcattga aacaagccaa agtaagatga ggtcttgttt
     3181 tccattctaa gtacttcgta ctaatttttt catcagcgat ttttttagat aaatcaaaaa
     3241 gttgcttttc cgttttttta tcataatata gctctatcgc atattgcata ataacacctc
     3301 caccataaag taaaaatacc tatctataaa aacacacaaa tacctatcat tttaatatta
     3361 tattactact gttcctcttc caattttcta tatagtgtag ccaaacctat gcctaagtct
     3421 tttgctatct tcttcttcgt ctttgtatct cttccatact tattcaacaa cttgtttatg
     3481 taagtcaact ctaattcttt aattgaaagt atcctttctt gattctctac tatccctcca
     3541 actatatcat cttcaaatat tatatttaca tccttatttt tataaatatt tccattaata
     3601 ttataatagt ttaaaatatc taatggaagc atatctttat atattattcc atcatctcca
     3661 accaaattca tcatatattc aactgcattt tccagttccc ttatatttcc cggccaatta
     3721 tagttaatca acatattcat tacattttct tctattttat ttaactgaat acctaattct
     3781 aaagaatatt tagaagcaaa cttcatcatt attgcctcaa tatcatcctt tctttcccta
     3841 agaggtggca aatttatagg aattacattc aatctatagt ataaatcatc cctaaactta
     3901 ccttcattta ctaatttcaa taagttttta tttgtagcag caatcactct tatatctaaa
     3961 tctataagtt tatttgaccc aattctcgca aattttctct cttgtaaaac tctaagtaac
     4021 tttgcttgta aaggcattga caaatctcct atttcatcaa gaaatataac accttcattt
     4081 gcaagttcaa atttacctac tttccctcca ctactagctc cactaaatgc accttttata
     4141 tagccaaata gctcactttc aaggagattt tctggaatag cagcacagtt tatcgcaata
     4201 aatggcttgt tccatctgct accttcagca tgtatagccc tagcaattag ctctttaccc
     4261 gtaccacttt ccccagttat aagtacagtt gaatttgact ttgctaattt cttagttctc
     4321 tcttttactt ttaacattgc ttctgaattt ccaattatag actctatatc actatttccc
     4381 cagccactat tcacttttcc atgtccttta tgattgatat atgtcttgtc aaatattatt
     4441 attttgtcgt attgatttat atatggatat accggaatta ttttggcatt tatattataa
     4501 attttgttat caatatcaat ttctagtaat tcatgtccat ttgaggaaga ttcaacagat
     4561 actatattta ctatattttc tattatattt ttatctatat ctaatttttt tagagctata
     4621 ttgtttatat aagatatttt gctatttatg tctagtatta taacgccttt ttcaacattt
     4681 tcaatgattt ctatcatcat atccattttt ctactgctat tttttgacgc ttcatgttct
     4741 tctattttca tagaaattaa atcgcatata tgattgacaa attttaaata attatctatt
     4801 tttgcactta tttctacttt tttagtttta tctgtgctaa tagcaccaat tactcctata
     4861 gttcgattat tatactttat tggaactgct attattactt tatttaggca tttcatgtaa
     4921 tgggaacatt ctatacaaag ctgattatca catatatcca atacgactaa ttcctgacct
     4981 gtctgtataa cattgtcata aataaagccc tcagtaacaa cgctttcatt tatctttgat
     5041 ttatacagac ctgtaccact aattcttatt aatctatcgt caactatttc aatatctaca
     5101 tctaacacac tagctactgt ttcagtgtat tttatcattt cttgttgaaa ttcttttaat
     5161 ttacttaaca tagtttcccc cattagaatt taatttacca aattatcagt ttagctatat
     5221 tttaacatat agtcactctt taaaatatat ataaaacatt acttatttat atatttgcgt
     5281 taaaacagac aatattaatt tataaaaagt ttgttttata tagtaacctt aattataata
     5341 tgcagacaat aaaataaatc acaatacaat aataaaaaaa agctttcctc atataacaaa
     5401 tatatttttg acataaaaat atacattata tgagaaaagc tcaatagtta aatatttaga
     5461 tagattttta tatagacttt tttaatataa acttttttaa ttgtagactt tttttattag
     5521 tttttaattt ttattagttt tgttattggt tttattgtta taatacttat aatactgcca
     5581 ttgcttcaat ttctaaaact gcatcttttg gtaattgagc aacttgtaca catgaccttg
     5641 ctggtggatt ttctttaaaa tatgtagcat atacttcatt aatatcatta aaatccttta
     5701 aatcttttac gaatactgta gtcttaaata ttttatccat acttgtacca gcttgttcta
     5761 atattgcttt tacattatct aaagaagctt ttgttgcttc ttttgggtca gatactaact
     5821 cgccagtctt catatctatt ggaagttgac ctgatgtaga aactatgttt ccaacttgaa
     5881 ttgcttgaga atatggtcca attgctcctg gagctttctc tgtagctatt actacttttg
     5941 acatgacttc acctcctatt attaagttat atttacagga aaataaaatt tcctaaacac
     6001 caatattaat acgaaccttt agattaaact tcaaatctaa aacttcaaat aatcaatact
     6061 tagatgatta tgtttatttg tgtcttgtta acttttatta aagcaattaa cgtgccaaac
     6121 tttatttata tataaaattt tgtgaagtca ttcaaaatag ttacttctga attttttcgt
     6181 aaaaaaacgc tctaaaatta cttttttatg atgtcaattt ttttctcata tctagaaatt
     6241 taccaacatg agaattcttg ttttgagaat ttacagatta aagttatttt ttaaccttaa
     6301 attctgaacc tcccatgact aaagtcacag ggttcctgct tcatagaatt ttgcataatc
     6361 aagttattac atatgaaaga actatagttg acaatcccac tattataaat aataaaacct
     6421 ttacccatac cttatttacc ctagttatca tagagcctat tgaaaatata acagctaaat
     6481 ataataataa ccaaaaaatt atcaatatct accactgttg ctgaaaatcc aaaaaactac
     6541 cttttgatag tattacttaa caatctaatt aatctcataa taaatttaca acttataaat
     6601 aaattgttat attggccgta tctcaacaac tttctccttt cctagtttta aatcttttat
     6661 agaattgtac cacgtctaag taaattttca tactattaag tcttatttct aataaatcat
     6721 tagctctcat tctatgccca ataatgtaag attgcagtaa ttttattgat tttaatagtt
     6781 ttatttaata tacttatatt aattattttc atatgaaatt attatttata tagggggatt
     6841 tttttaatat gagcaaaagt gaattaacag cagaaacaac agaagaaatg ttagaagtac
     6901 taagtggtaa agattatgat attgcatgtc atttacatga acttggtaaa tcattagatt
     6961 gtaaaattga accaaaaaca ggtgctcgtt cttacaaaat agtatattca actaagaaac
     7021 caaaacgtag cttatttact attgaatgta atgaaaaaaa atggagagtt aaagcaaatc
     7081 tttttcatct aaatacatat aaagatgctg tggaggaatg ctctaaaact attaaagata
     7141 gtataactaa aactcgtact tgtaagaaat gtaattcaaa gtgtattgga ggttcttcgt
     7201 ttgaattaga tggaaagtct tacctgactt gcataggaag tggtcattat tttgcaaata
     7261 tggaagaaat ggattggaaa aacctagaaa aattaattac taaagaaaat aatattatgc
     7321 aggaatctgt atagtttttt aagggtaatt attaaaatat atgagtaaca acagatactt
     7381 aaataggcat aaaaaaataa agggtataat aaggatatag aataaaaata aatgatatag
     7441 cacaaagagg ctgtctcaaa atacagataa gcctcttttt attgttgaaa tgataagaat
     7501 atattcaatt ttatacaatt ttatacaaac atactacgcc aacttaccca tttcatatgc
     7561 ctgtttcatt gccttacttc ccttaatatc acctacattc caagcaccta caccatatac
     7621 tatacctttt tccttagaac catttagaca tgatgtaaat cctctaaatc cttctattgt
     7681 tctttccata tcagccacat tttcatctgc tgctgtaacg ataaaataga aatctttatc
     7741 acaaatttca gtatatctag agcaagttct atcaatcata gttttcattt gcgcattcat
     7801 agtataaaaa taaactggag tcgctaaaac aatcacatct gccccaatca ttttttcaag
     7861 aatttctccc atgtcatctg tctgtgaaca atttgcttct ttttcataac aagcaccaca
     7921 tccagtacaa tagttaatat ctttatcttt taaaaatatc ttctccactt gatttttaga
     7981 ttctaatacc ccttgtataa actggtcaca caataaatca gaatttccac cttttctagg
     8041 actagctgat actattaata catttttact cataagatac tgcctccatt ttcttatttc
     8101 tactgctctt taatactttt tctttctcaa taataccttc ttcatatcta tcaatcttaa
     8161 aatttaatcg ttccaaagtt ttttgcattt cttctattct ttttgctagt tggtttctct
     8221 gctctattaa aagttctttt ctagctttga tagtactgct cccttgttga aacatagcta
     8281 catactcaat taatgtctca atagaaagac ctgcacctct catacacttt atgaaattaa
     8341 cccactcaca atcttcttct gtaaaagacc taattccact tttatttcta tttacaggtg
     8401 gtattaaacc aattcgttca taatatcgta aagtatccgc tgaaagctcg tattttttac
     8461 taacttctga aatcgtcata catatcttct cctcttgata ttgtatagat tgaattattt
     8521 ctcaaaggtt tctgctacat tctttaaagc atctataatt ttaatgtgtt gaggacagct
     8581 ttcttcacat tgtttacatt caatacaatc tgaggccttt ccataagttt tagtataatt
     8641 attatagtag accatctgtg ttgaaaatcc agtattaaga gcttgttttt ctgcattgta
     8701 caacgcaaaa tagtttggaa tagcgatatt ttttggacag ccttctacac aatactgaca
     8761 ggcagtacaa ggaattaaaa ttgattcgtt gataatctct actgcttcat ctataatatt
     8821 atattcctct tgtacgaatg gtttgaaatt ttgcatatat cctgtattgt ctaataattg
     8881 ttccatattt gacatcccac ttaagaccat cataacatta tctttacttg ctgcaaaacg
     8941 tattgcccaa gatgaaattg acatatctgg attgtagtca ttcaataatt tctctgcttt
     9001 ttctggtatc tttgcaagtg tacctccttt tactggctcc ataacaatta caggcttatt
     9061 atgcttctct gccacctcgt agcattttct agattgtatg ctttcattat cccaatctaa
     9121 ataattaatc tgtaattgca cgaagtctac ttctggatgc tcactcaaga ctttatccaa
     9181 taattctgca ctatcatgaa aagaaaaacc aatattcttt atttttcctt cttttttctt
     9241 ttcttcaata aacttaaagc tatcaaattt tttagctact tcataatgtg acacgcctat
     9301 attgtgtaaa agatagtagt caaaatagtc tacaccacat ttttctaatt gctcattaaa
     9361 aatggtttct tgctcttctt ttttctttaa agccattaat ggaagttttg tagctatagt
     9421 aaaactctct cttttgtgtc tttttactag agattctcta agtgctatct cactttttcc
     9481 catgtgatac acataggccg tatcaaaata tgtaaatccc ctttctaaaa aagtgtctac
     9541 cattttattt aaatgttcca tatcaatagt tgtaacatca ttttcatctt ttattggtaa
     9601 acgcattaat ccaaatccta atttttttgc ctccattgtt ttacttcccc ctttaaaact
     9661 tgtttgtttt ttctctctac tttaactatt cttacaagta tataattctt gtataattat
     9721 actttactac ttggagttaa cttcaagtca atagattttt ttatttttaa gacaaatatt
     9781 ataaaaatac agttctttaa tattaatcca ttacatgttt ctttcaccct ccaattctca
     9841 aacttcacaa actccaacat gtaaagaaat atatacttta agaaattcag tccattaata
     9901 ttactacaaa tactttttag atatgtttaa aaagaaattt cacctaaaat atttataaaa
     9961 gatttagaaa taggtcttgt gtctatttta aaatatataa aacagtgata aagacttatt
    10021 tataagataa aaaatgaata gtgtataccc tttttaaaaa gttttttcct ttttatattt
    10081 gtgatacaat gttaaatata aaaaggaaaa ttttgtatta aagaaatctt atttgttgat
    10141 tttgtaactt caaactcagt atcaatttga ttttaagatt atattttatt aagtatgcta
    10201 aataacaaat ttttaaagat tgtgaggtaa aaatatgaat aagaaaaatt tagctatatt
    10261 aatatcaggt atgatgtttt tttctagttc ttcagcaatt tttgcagaca atactacaaa
    10321 gcaagaaaga ttaataggta aaacaagata tgaaacagca gtagaagtaa gtaaattggg
    10381 atgggttcaa tcaaaaacag ccattatagt taatggtaat tctattcaaa gtgcgctttg
    10441 tgcaaatcca tttgctaagc taaagaatgc acctatactt ttagtaaaca acaacagtat
    10501 tgaaaattca actaaggctg agcttaaaag gcttggtgta gataatgtgt acattgtaga
    10561 tagtggaaat agcatttctt ctaaagtaga aaatgaaata aaatcactta acataaaaat
    10621 aaataaaata gtaggaaaca atatatatga aatgtctaca aatgtactta aagaaataga
    10681 taagataaaa aagatagaaa atgttgcagt tgtaaatgga ctaaagggac tatctgatgc
    10741 agttagtata gcttctccag cagcaataca taatatgcct ataatattag tatcagatga
    10801 aaacagcaat gatagtagta taaatttctt aaacaatagg tctataaaaa aatcttacat
    10861 agttggagga aataagattt tatcagacaa tacagtaaag aaatatccaa acagagaaag
    10921 aatcagtggt tcagatagaa acaatactaa tgcaaaaatt attgataaat tttataaaga
    10981 taaaaaaata aataatgtat ttgtagctaa agatggtatt aaccaagaaa atgcattagt
    11041 agattcatta tcaataggag tactagctgc taaagaaaat tctcctataa tgatatctaa
    11101 tggtagcctt gtaaatagtc aaaaagaatt tattaaaaat aaagatatag atgaaataac
    11161 acaagtagga ggaggatata atcaaaaagc atttgaagat attttaaaac taaaaaatgt
    11221 gaacataaga aataaaacta aaaaaatatc tttaatacag gctaaagaaa tattaaaaca
    11281 agctcttcca ggaagcaata ttttagtttg ttattatgat gaagatgata atgaatatga
    11341 ctgcaaagtt ataaaggata atatcctata tgagatagaa attgatgcct actctggtaa
    11401 aataatagaa attgaaaaag atgatgactt aaatgataat aatttaggta cacaaaatat
    11461 atcattggat aaagctaagt ctatagcatt agatgctatt cctaatggta atttaatcta
    11521 ttgtaaatat gatgctgaag ataacgaata tgaggctaaa gtaataaaaa ataagaaaac
    11581 atatgaatta gagataagtg catttgatgg aagaattgta gatattgaca tggacaatga
    11641 ttctgatgat gactttgatg ataatgataa gaataactct aatactaatg acaacagttc
    11701 tattattagt gctgaaaaag ctaaatcaat aatgctagat aaagtgcctg gtgggaaaat
    11761 agtaaaattt aagtatgatg aagatgacaa agaatatgaa ggtgaaataa taaaaggaaa
    11821 aactgaatat gagataacaa ttaatgcaat tactggtaaa atattagaat ttgaaataga
    11881 tgattaataa aaaatataaa tagatgatta ataaaaaata tatattctat taattattac
    11941 aagcaataaa gccataaata ttgaaatttt caatatttat ggctttattt tattttttta
    12001 atttaaatcc acattatgcc attatatata tctatgcact cttgtaaaat gggttatatt
    12061 tctccagtat attaaaaaaa gtgagtgtct caaaataaat cttctaactc ataaagcact
    12121 ctcctttgta ttaaatcaaa tatattttca tcatttaaac aattaagaag agacttctct
    12181 ctattttgag acaactccat tttaatacac tatttataca taaacatatt ttaaacaact
    12241 aaaaagcaac taatatctta ataaatatct caatacatat cttcataaca catatcacca
    12301 taattacaag cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac
    12361 agaattgttg gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc
    12421 tctataacct tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt
    12481 gccttagtta tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt
    12541 ataataattt catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca
    12601 tccgaatttt caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat
    12661 tcttttttct ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct
    12721 ttgtctatac tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac
    12781 tcatttctat gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat
    12841 ttctctatat ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca
    12901 gaacaaacat aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc
    12961 aatataccta aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca
    13021 aggtcagatg cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt
    13081 aatctttgta tattttcttt tctcattaca tagtgattaa accttattct atttacatct
    13141 cttttttcat taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc
    13201 ccactcctcc aatttaaata tataatttgt tgtaaagttt ttagattttt aaataactta
    13261 ttaaatgatt atttttatta aaaagtattc tttgatattt tcattttaaa gttttaagta
    13321 taataagtta atagtgtaga tttaactttt ttatttaaac aataaactta atattatttt
    13381 taatataaaa attttattaa atttagtatg taatttttta atcttatttt attccatcta
    13441 tactcttatg ctaagtaata actttgataa tatttcccta acttatgttt caactggttt
    13501 atattgcaat ttatttaatt acagcttatt tgcataataa ttcgtccata tcataagttg
    13561 tgtatttata taaatacaca attaaaaata aatcactatg gtatactaat tattacgaag
    13621 actatactta tatgcaaaga caaatataag gcatcttata tcttttatca attataaaaa
    13681 attaaacgct atcttattta tcataaatta caaaaacata aaattttatt actatattgc
    13741 tataaattaa ttgatacaca ttttacacaa taaggagggc tatatatgac aaatgctaaa
    13801 agtaaaaaag aatatctaaa tcgtatttgc aaagtgcaag actatataga agaacattta
    13861 catgaacctc tttctcttga tgaactttct aatatagcag gtttttcaaa atttcatttt
    13921 catagaatct tcaaagcaat agaaaaagaa actttggcac aatatgtaaa taggctaaaa
    13981 ctagaaaatg caactgcttt tcttattcat agaactgaca tgactgttac tgatattgca
    14041 cactacttcg gctttactga ttcagcagtt ttctcgagag catttaaaaa ttactataaa
    14101 ataagcccta taaaatatag aaacaattat agcaagaatt gcaaagaacc atataaaatt
    14161 tctcagtaca atagaagcat atcaaaacct aaatgtaaga atactagaga ggttaaaggt
    14221 gaaattgaaa ttctagaact tgataatatg aatactattt acactagata tactggttca
    14281 tatggtaatc taaccagtac tttcccaaaa cttttagaac ggttattcac atatgcaagt
    14341 gagcagaatc tacttggact tgaaaacaca caaatattag ccatttatca tgataatcca
    14401 caatttacac aggagcatca attaaggact agtatttgca tgaccattcc agataacatt
    14461 gttgtcaaag agaatgacaa tcttggcaat atgataatac cttctggaaa atatcttgtg
    14521 ggacattttg atatttcaaa atctgaatat agtgatgcgt gggattttat gtataatgaa
    14581 tggctatcaa atggtaatta taagctacgt gcctccttcc cttttgaagt atatcttaac
    14641 aacccacaca atcaccctca aaatagacac ttggtcgata tatatttacc tatagaacct
    14701 ttttaataac ctcacctaat ctattcttgt attaaggatt aatatatccc tattttacgg
    14761 tttgataaaa ttgataaaat gaggtgaacc aatgaaagaa ataaaaatta atggagcaaa
    14821 gatacacaat cttaaaaata tagatgtttc tattccaaaa gataagcttg ttgttgcaac
    14881 aggtgtcagt ggctcaggta agtctagcct tatgtttgac attgtatttg aggaagggcg
    14941 taaacaatat ctgcaatctt taggtatact tgcaggtatt gatagtgaag ataagtttga
    15001 taatattcaa ggtttagctc ccactatagc tgttcaacaa aatattattc gccagagcaa
    15061 tcctcgttca acagtaggca caagaaccaa tattctcaac atgcttacac tattgtattc
    15121 agtggaaggt caaataatgt gtactatgtg tgatactcct gtaagcgata atctaatttg
    15181 caataattgt ggatatgagg aagaacgatt aagaccaagt tatttctcat acaactcttc
    15241 agatggaatg tgtatgaagt gctctggaca tggtgcatat tttgagatta atattgaaaa
    15301 attagtcctt gataagcatg ataccctaaa acaagtctta gatagagcaa agattacacc
    15361 tggatatttg agagtatttt ctaaaaaatt caatgattat ttagacatac aattcttaca
    15421 actacctgaa gaaataagaa atgaagtact ctatggtcat tatgaaaatg gtaaaaaaag
    15481 ttcctcttta tcaaaggtat ttcacaatcg ttacaaaagg ggcgaagatt taaatggagt
    15541 ttatactatg actgcctgtt cagattgcaa tggttttaaa attggtgaag aagcacgtag
    15601 agtactttta aacggaaaac atattggaga acttggaaaa atgacgattt tagaaattga
    15661 tgatttcctt aaactcttaa taaatcaagg taatttaaac acatttggta caaatcttgt
    15721 aaatgatatt ttatccaaaa ctcaacattt aatagaatca cgattaggtc atctatcctt
    15781 ataccgagaa atgtcaactt tgagtggtgg ggaaatgcaa agactatttt taaattcaca
    15841 tctcgattca gagatggact cattaattta tgtgcttgat gaaccaacag ctggattgca
    15901 tgaatcagaa aaaatagaca tcttaaaatc actaaagaaa cttaaagatt taggcaatac
    15961 ggtcatagta gttgagcatg ataaaaacac aattgaaatg gcagaacata ttattgatat
    16021 tggtccaaaa gctggtgttg aaggtggtca attagtatat caaggagatt tagaaggtct
    16081 acttcaatcc gacaaatcac ttactggaca gtatctatct ggaaaatatc caatgcctaa
    16141 tagaacaagc aataaaaaca ttacacatac tgataaaata ccttgtataa ctattcaaaa
    16201 tgcaaataca aataatctta aaaatgtaac tgtatcatta cctttgggtg ctatggttgg
    16261 aatatctgga aaatcaggta gtggcaagag ttcacttata tcagacaccc tacttcctct
    16321 tcttagaagc caatttaata accatactat caataatcaa ataaattctt cagagagtga
    16381 aataggtgag gaagacgatt atgcagttgt tgaaactatt gcagatagac tgattggtac
    16441 taaacatatt tctggatatt cagagatatc tcaatctcct ataggcaaaa atatgaactc
    16501 aactcctgct tcttatattg gtatatggga taagatacgt acactatttg caggtcagcc
    16561 agaatcgtta gaacaaggat ttacagctgg tcatttctca tttaactcca aaggggcttg
    16621 tcctaaatgt agtggtagtg gatatgaaaa ggtctggttg ggaaataatc taagtataga
    16681 ccatatatgc agtgaatgtc agggaaaaag atttaatgat gaatctttat ctattaaata
    16741 taaaaataaa aatatacatg atattttaaa tatgagtgtt tctgaatctg tacatttctt
    16801 caaagatatt ccaaatattg tttcacactt aaatgtatta gaacaaatag gtatggggta
    16861 tataaaatta ggccaaccta caccgacttt aagtggtggt gaatcccaaa gaatcaaact
    16921 tgcaaaagaa attggtaaaa aacgaaatgg taatatcctt tatgtattgg atgagccaac
    16981 tactggtcta agtctatatg atacttctaa gttgattcaa ttattagatg aattggttga
    17041 aaatggaaat tctgtaattg ttgttgaaca tgatatagat gtattaaatg tttgtgactg
    17101 gattgttgag cttggtccag aaggtggtga caaaggaggt tatataattg cagaaggttc
    17161 tccaaaaact ctaaaagaaa atccaaaatc tataacaggg aggtatcttt aatgaataaa
    17221 aacaattgct tagacttaat ttttgaaaaa gtagaccctt caagtgttgg tatgagttca
    17281 gaaaagcttt cacaacttga acctttaata atctcacaat atagcaatat aaatggtata
    17341 attattgcaa gaaaaggaaa aattgtcttt gaaaagtact ataatggata taatgctgat
    17401 gatacttttc atgttgcatc agttacaaaa agtattatat cagccttaat aggtattgca
    17461 atagataaag gatatataaa agatgttgac caaaaggttg ttgatttttt tcctgaatat
    17521 acttgtagtc cttctgaaat atgtaaaaaa gcagtaacta tacgtcacct acttacaatg
    17581 actgcaccat acccttttaa aaatatgact gagtctttag aaagactttg caaacaaaaa
    17641 gattggataa aatatacctt gaatcaactt ggtcaaaaag gcgaaattgg tactttcaaa
    17701 tattcttcag ctggaacgca cctactttca gctattataa caaaaactac tggaaaatct
    17761 gctcgtgaat ttgctaatga gtacttattt aaacctattg gtatgaaagt tataccagat
    17821 tatgatatga aagattttgg ttttgaagat ttctttggaa aaaatcttaa gggttgggca
    17881 aaagatagac agggttattc tataggtggc tggggactta cattgagtgc tagagatatg
    17941 ttgcgatttg gttttctgta tttaaatgat ggtatttgga ataataaacg tattatatct
    18001 aaatcgtgga ttgaagaatc agttaaaatg aatagtaatg aatacggtta tctgtggtgg
    18061 ctatgtaaaa aagatggtgt tttgtggtat tcagcaatgg gaactggagg aaatgctatt
    18121 tgttgtattc cacaatatga cttagtaata gccataccat ctaaaatagt gtttaaacct
    18181 cgtgatagat gggagttcat tgaaaaatat atacttactt ctataataga ttaattactc
    18241 tttcataaac taattactct ttcataaact aattactctt tcataaacta attactctta
    18301 gatagccaaa ccctccatta taacaccaga cttttgtttg gtgttattat gactaatact
    18361 atatatctaa aaggagttga atactaatgg acacttacac gacatcagag gtagcagatt
    18421 taattggtgt acatcctaat acagtacgtt tatatgaaga actaaactta atacctaaac
    18481 ctaagcgtaa taccaatggt tatcgtatct ttaatgattt tcatatagaa caatttagat
    18541 ttgctcgtac agccttgaaa attgaagtgg tacaaagggg attaagaaaa gatgcccttg
    18601 agattgtaag aacatcagcg aatagggact ttgataaggc tattagtctt gtaaatcaat
    18661 acttgcaaca attagaaata gaaaaatcta atgccgagga agcacttact attgttgcac
    18721 agattttaaa tggaaatact ccagaaacaa ataaactctt tttaactaga aaagaaactg
    18781 cagaacactt aaacgttaca atagatactt taagaaattg ggaaatgaat ggtttgttaa
    18841 aagtaaaaag aaaacaaaat ggttatcgtg tatatactga tgaagatata aaacgactta
    18901 aaattataag cgcacttcgt tgtgcaaact attctctttc agctatttta cgaatgttaa
    18961 atgctttatc ttctagtcct aatactaata ttaaaactgt ccttgacact ccaaaagatg
    19021 atgacgaaat tatctcagtg tgtgacaaac tgataacttc attgaaagaa gctgaacata
    19081 atgcaaaaac catgaaatct catcttcaaa ttatgaaaaa acaattttcc tagccctcca
    19141 ctctaactcc agactttgat ttgtgtgtta tccttaagag caagaaaaat tcaacacaca
    19201 aatcaaagga ggaataggat ggaaaataaa aatactaatt ctacatggaa gaaaaaagtc
    19261 attctttttt tagcaagtca atatatttcc ttatttggct catcacttgt acaaatggca
    19321 atgatatggt atgtcacact tcagacatca tcaggtgtat gggtaacagt tttaactgta
    19381 tgctccttta tacctcaaat gataatttct ttttttgctg gtgtatgggc agactactac
    19441 aatcgaaagg tactcataat tgtatcagat tcaacaattg ctatatctac attagttcta
    19501 attctattta tgatgtcatc aaatggcaca acaagtgaat taatagccat atcagtaata
    19561 tctgttatac gttcactagg aagtggtgta caaacacctg ctgtaaacgc aatgattcct
    19621 caacttgtac cagaagaaca tttaatgcgt tttaatggta taaatagtag tctacagtct
    19681 atagtacaat ttatagttcc tattgtagct gctgcaatac ttggatttgg taataaaata
    19741 aatcaagttc ttttaattga tgtatttact gcaattattg ctattgtatt attaatattc
    19801 atcaaaattc ctaaacatga tataccaaaa caaactgaaa aagtctcatt ttttgcagat
    19861 atggtatcag ggataaagta ctgtttttca gataagttca ttagaaaagt gctttttact
    19921 tatggagcat tcatgcttct ttgtgttcca tcaggatttc ttataggact tcttattgaa
    19981 cgaacatttg gcaagggtta tatatacctg tctattattg aaatgattgg atttttaggt
    20041 atggttttag gtggattgat tataggagtt tttggtggtt ttaaaaatag aaataagacc
    20101 ttttttctag caatattatc ctatgctatt ttttcaatta ttctaggtct tgtaacacaa
    20161 gcttggcaat tttttatatt tatgtttttt acgaattttt caattccaat cattcaagca
    20221 tcagctatga ctatgttgca agagcatgta gcaccacaaa tgctaggtag agttcttagc
    20281 ttacctatta ttatatatac tggttttatt cctcttggta tgatgatttt cggaccaatg
    20341 gcagatgtaa tttctattaa taatttaata atcatagcag ggattatact tttatttttt
    20401 gctttggcta tcccctattc aaaacaattt tacaagcaag gaatatctaa gtcaaatgaa
    20461 tccgagtaat atatttaaat agtttaactc gatttatctg gttaaaaatg aaggggctta
    20521 aaatggactt tttaatttat gaagaaaatt ttttatatga aatcaaatat attttatcat
    20581 ttcaacattg aaaaataggt ttatctttat tttgagtccc tctctttata gcaaataata
    20641 ttttgatact atctttatta actttatata ataatacctt tacttgtagg ttataatgat
    20701 gaaatttatc aaatcatata ataatacctc catttatatc ataaattaaa tttatagtac
    20761 aaatggagga aaataaagta tttatatagt ttaagtacct attaagtttt ccactaagta
    20821 gctctaattg tactgaactt cttactaaat tatattgaat aattataaaa taaaactaac
    20881 tattacacta cataaattaa agtatacaaa ttgcactaca taaattacac tatacaaagt
    20941 atattaaatc acctattaaa ttgtcccaat tataattata ctgagttatc tactaaaaga
    21001 taactttctt tttttatcta aaataatata tatcataaac catactacac aatgtacagc
    21061 caaaattaca atatctttga taataatgac attaccatta ataatctcca taataccatt
    21121 aaatgtagca actgaaggca ttatatagca taaattccct ataccactgg tattaacccc
    21181 tagaagatat attaacaaag gagtacctat aaataaaatc attactattt taatataaac
    21241 aattcctacc ataagacctt ctgacaactt tccaataaat aaacctataa gtgatgaaat
    21301 aaatgctgaa aatacaacga gtaacaacat cattatcata tctaatattg aaatatccaa
    21361 acatataaat acagttatca ttgaagataa acacccacat aaaagaccca aaaatacttt
    21421 ttgtataata tactgacttc tactctgtgg caaaacttca tttataaatg atattccatc
    21481 ttctttttcc gatataatat tcattgcatt aaaggtgcat cccataaaca tagcagtaat
    21541 taaaatcata gcaataaaaa cattttgaaa ctcttttaaa atactatctt taggcaatat
    21601 tgtaacctca acatctttta aatcaccctt tagtctaaac atctctggca atgtctttcc
    21661 aacatttttt atagcattaa tctcatttcc ataaaacaca gtttttattc ctttaccatc
    21721 tctaacaact ccaattgtgt ccgttttaat atccttaatt tcacgtatta aatgcatttt
    21781 tgtgtcatat acacttacca taccatactt ctttaaccac tgctctgttt gtacaggaag
    21841 tgtatccttc actataccaa aacgtatctc tcctaatgaa gatatatcaa ttgttcccat
    21901 aaaatttatt aaaatcgcca taacaactgg taaaaagaag gaaattatac acattttatc
    21961 tttttttata ctctttaact ggtaacgtaa tcctatcaag attatccctc cctaaccaat
    22021 tctttattaa aaatatattt tacaagtaaa aatagaacta ttattgtcaa agaacacact
    22081 acaaaataaa ctaattcttt tgaaaaattc atgaaaaacg catttttaag ctgactataa
    22141 acatgataga agggatgata tttaatccaa tctatttcaa aagatgtatt tgcagataga
    22201 aatataggtg tagttatgaa tacagcaacc acaagatata taagagaaaa ttgcttaaaa
    22261 tctctcaata acattgagca tcctattcca atcaaagaca taataattga taaaatacta
    22321 gcttgaacta atacttgtgg aagtattttt attccttcta aaaatccaat attaaatact
    22381 actaaaagaa ctgtaaaaca taggtcacat ataaaaaaag caaatatctt tgataatata
    22441 aaaagacttc tttttacagg taaaatacca gtaatacgca ttactcccat ctgcttttct
    22501 ttaaataata aagaggcaat ccctaaaaat ccaatagcta caatttcaaa gaataaaatc
    22561 tcacaagtca tgtcttttct ttgttttatc tcagatgtat ccttatcatt aatttgaatt
    22621 attgcattgt tgtctgatat tttgctatta tggtcttttt ctgatgataa taatgacaat
    22681 gcatagtcag ctctataatt gtcaactcct ttaatacttt catgtaaaat tactttcggc
    22741 ttactggaac tcatatcaat acaaactgca cttgtatctt catcaagttt tgcttttaat
    22801 tcacctatag aagaaacttg attaattaat ggagatgcac tcatgcttat attctctgga
    22861 ttatatagat atacattatt gtgataacta tcttcatcca taaactttac ataaccaaaa
    22921 ttaatataac acgaataaag tatgagagag ccaagtgtca gcacaaaaaa tttaccataa
    22981 atcatcatcc aaaaatcttt ttttactaag gtaaaaaatt tagaatacat tacactaaca
    23041 tccttcctgt gtatttaata aaaacttctt ctagtgtagg ttcttttgaa tgtacactta
    23101 agatttcatc atatttaaaa gttattccag attctaactc ttgtatatct acaatttcaa
    23161 tatctcgatt tccattatat acaaattcca cttcaacttt attattgcta tttttcattt
    23221 ttaaattttt gggtttatct attaccataa tctttccatc tgcaataaaa gccacattgt
    23281 tacacattaa atctgcatct agcatattat gagtagtcaa aaagatagtt gttccttttt
    23341 ttcgttcttc ttcaataatc ttacgaaata gtacagctcc agatgggtca agtccactag
    23401 taggctcatc taaaaataac aactttggat tacttaccaa tgctcttgcc atgctaatac
    23461 gttgcttcat accttttgag taggatgata caggcttatt tataaactct ttcttaaact
    23521 ctaacaaatc taagagttca tatatgtctc tcctttgatt tttaggataa aaagacgaaa
    23581 aatattctaa attatcaatt gcacttaaat tggtatataa ataaggaaat tcaaataaaa
    23641 caccaatctt ttgatataaa gattctgtgt atgctttaac ttccttacca aaaatggata
    23701 cgtgaccatt gtaacctttt aaaacaccat ttaagatttt ctgtgttgtg gattttcctg
    23761 aaccattagg tccaaggaat ccaaagattt ctccttcttt tacttcaaaa ttaagtccat
    23821 gcaatatctg tttatctttt ccataagaaa attttaactc tcttactttt atcatctatc
    23881 ataacctcct ttatcatttt tttgtcgtct atcccaccat tttataaatt taattttaag
    23941 cggaatagaa agcaatatta tcaatataat agtcaaccat gtataccatt tcataagtac
    24001 tcccccttgt aaccttattt aattacgctt ctatttttta atttatttaa cctattttat
    24061 ttctatttgg tgaaatcaga gtgtctctct tgtgaaatcg aagtgaaata ataaaaagct
    24121 taataaaaaa tcttgttttc atcaggaaaa caagactttt tattaagctt ttcattaagc
    24181 tttttattag actttttcat taagcttttt cattaagttt ttttatttta ttctctttaa
    24241 atacttacgt atacaaataa aaatatttta catcagatac ttataattta taaattaata
    24301 ctaaattatt catcaattgg aatcataaaa taaaactcta ctcctctatc acgattattc
    24361 actccatatt ctaacccttg cattgtaagt atttgtgaaa caattgcaag ccctagacct
    24421 gaacctcctg tacgctgaga ttgaacacta cgataaaact tagaccaaat ctttgaaatc
    24481 tcatcttgct ctataaacct accatcatta taaatttcaa ataaaagcaa gccattttca
    24541 catgtaatcg ataaatctaa attaccatta tatacaacat gcttctttgc attaataact
    24601 agattctcta ctacttgttc cattctcatt ttatctgcca ctacaaaaac ttcatactct
    24661 ggcaaatcat atgtaaaata aaaattggca tctggtatat caattaaaag acgacctgcc
    24721 acagtttcaa caaactctat aaaatcaaaa cgctctgggt taagtttaga aactcctgct
    24781 tccaatgcag ataagtctaa taatgataca atcatattgt tcattctatc agtttcttct
    24841 attataacat tcatataatt tttctttttt tcttcatcaa cttcatctat aagcccctct
    24901 gtatacgcac gtataatgcc aagtggagtc ttcatttcat gtgaaattgt atctaccaac
    24961 tcctttcttt gttccaatag cattctttcc ttatttacat ccttttcaag ttgaatattt
    25021 gcactttcta ggtctgataa tgcctgttgt agatttgtag acattgtatt caggctccta
    25081 gaaagttctc caaattcatc attagtattt acagtacatg gattagaaaa atcaagttgg
    25141 gacatacttt ttgcagattt atttatagat gtgattggtt ctgaaataaa ttttcccaat
    25201 aagaaatcaa tactcataat aaatataacc agtattataa accaaatcca aaattcaaat
    25261 ttagagttta tcgaaaattc ttcaacaacc agataaaata atataattaa cgctcctgat
    25321 aatttagaaa gtaaaaacac tttttttcta actgaatata gtccaaaatt ttgtttaatc
    25381 caatttgaaa tatctacatt tttcagtttt tctataatct ttcttaaact attcattcct
    25441 taacctcaaa cttatatcct gaacgaatta atgtaactat atggtgtcct tcactaccaa
    25501 gctttttgcg taaggttttt atatgtgtat ctacagttcg tgtgtttcct tcaaaatcat
    25561 atccccatat acaatttaaa agtttctctc tggaaaaaac ttgattaggg ttttccataa
    25621 ataaacgcaa taattcaaat tctttatatg ttaaatcaat caatttatct tctacataca
    25681 cttgtcttga tgctaccata ataaaaatct ttcctaaact caatgaattt ttgggaatat
    25741 cagtagtgta tctacgtaac aaagcattaa cttttgctaa aagtactttt gggctaaatg
    25801 gttttgttat ataatcatct gcgccatatt cataaccctt taatttatcc tcctcttcac
    25861 ctttagctgt caatataata ataggtatat tagtcatttc acgtgaaact ctacacactg
    25921 aaaatccatc taaatacggc atcataatat ccaatatcat taaatctata ttgttgctct
    25981 ttaatattgt aagtgcctcg actccatcat cagcagttat acaagtatat cctccctttt
    26041 gcatatattc tacaataata ttttgcattc ttttttcatc ttctacaact agtattttag
    26101 ccatttctat ttatcacctc ttttttgtag gctttatttt agtttatatt aatttattta
    26161 tttttcaact atttttttat agtatttttt atagcatttt gatagtattt tttagactat
    26221 tcttaatgga attgatatca tagataaaaa tagcctacta actttttcta tgttagcagg
    26281 ctaaaatatt tctaataata attatattat tttgtcacta ttctattttt tcgattaaaa
    26341 tatattctaa agcaaatatc ttcaattaat gaataaattg cacttgttat aaagtacaga
    26401 cctatggcta ctggagttct aacagttagt ataagacttg ttatggtagt caccacagcc
    26461 atttgtttgt taaactttac ttgagaattt atttttaaat atggtatata attcactaaa
    26521 tttgaagcta acataattaa tgtatatata caaggtaata taaacaaatt atcaaatgta
    26581 cctaaattag caatccatgg aattataatt gttgtaaatt cctttgtcat atctaaacat
    26641 acatgatata aagcatatac tactggcatt tgaaccaaaa ctataagaca tcccagcata
    26701 ctcttcatac tttctacaga acttacttgt atttgctttt ccaattcttt agtatcattc
    26761 ttatactttt cttttatata ttccattttt tcagctaatt cttgctgttt ttttacagaa
    26821 aatctttgtt ttaatgatag tggcataagt actaatttaa ctattaaagt tataagtaca
    26881 atagaaattc caaaatcacc tgtaaaatta aataatatac ttaataatcc ttgcaatata
    26941 cttgaaatga aatccatttt attctctcct ttttattttt aaactttaga atttataata
    27001 gattcatttc aaattaaaac aaactacaat acctccaaat atcatatttc tgaaaaacta
    27061 tatactgttt aggcagtcgt tcttcagaat atgtaattct atttacaaat atgtctctta
    27121 ttaaaacata cttaatttta tgatttaaga ggtctttaca aaaataaaat atacttccaa
    27181 tgatagatac caataatgtc atataaatta gtacatataa catactatcc atatctaatc
    27241 tcatcacatc acctcttcat cacttgtatt tatttttaga caatttcaag tcctaaataa
    27301 ttccaacatt tatatttagt tttaaataat tatagtatag aaaactccta atgtaaatat
    27361 atatattat
//
LOCUS       DAMKMI010000039        26727 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMKMI010000039 DAMKMI010000000
VERSION     DAMKMI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26727
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.040"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(216..1466)
                     /locus_tag="PC429_001578"
     CDS             complement(216..1466)
                     /locus_tag="PC429_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF3847090.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1499..2440)
                     /locus_tag="PC429_001579"
     CDS             complement(1499..2440)
                     /locus_tag="PC429_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF3847091.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
     gene            complement(2680..3756)
                     /locus_tag="PC429_001580"
     CDS             complement(2680..3756)
                     /locus_tag="PC429_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF3847092.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
     gene            complement(4129..4644)
                     /locus_tag="PC429_001581"
     CDS             complement(4129..4644)
                     /locus_tag="PC429_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF3847093.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(5007..6077)
                     /gene="leuB"
                     /locus_tag="PC429_001582"
     CDS             complement(5007..6077)
                     /gene="leuB"
                     /locus_tag="PC429_001582"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF3847094.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGAVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTRAMGELIVNEI"
     gene            complement(6084..6575)
                     /gene="leuD"
                     /locus_tag="PC429_001583"
     CDS             complement(6084..6575)
                     /gene="leuD"
                     /locus_tag="PC429_001583"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF3847095.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(6590..7867)
                     /gene="leuC"
                     /locus_tag="PC429_001584"
     CDS             complement(6590..7867)
                     /gene="leuC"
                     /locus_tag="PC429_001584"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF3847096.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAKLKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAIIGKISKPSEII"
     gene            complement(7824..9566)
                     /gene="leuA"
                     /locus_tag="PC429_001585"
     CDS             complement(7824..9566)
                     /gene="leuA"
                     /locus_tag="PC429_001585"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF3847097.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(9690..9936)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(10084..10530)
                     /locus_tag="PC429_001586"
     CDS             complement(10084..10530)
                     /locus_tag="PC429_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847098.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(10551..11270)
                     /locus_tag="PC429_001587"
     CDS             complement(10551..11270)
                     /locus_tag="PC429_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF3847099.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(11388..12662)
                     /locus_tag="PC429_001588"
     CDS             complement(11388..12662)
                     /locus_tag="PC429_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF3847100.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(12862..14229)
                     /locus_tag="PC429_001589"
     CDS             complement(12862..14229)
                     /locus_tag="PC429_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3847101.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(14479..14943)
                     /locus_tag="PC429_001590"
     CDS             complement(14479..14943)
                     /locus_tag="PC429_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF3847102.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(14948..15577)
                     /locus_tag="PC429_001591"
     CDS             complement(14948..15577)
                     /locus_tag="PC429_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF3847103.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(15611..16498)
                     /locus_tag="PC429_001592"
     CDS             complement(15611..16498)
                     /locus_tag="PC429_001592"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF3847104.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(16527..17765)
                     /locus_tag="PC429_001593"
     CDS             complement(16527..17765)
                     /locus_tag="PC429_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847105.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(18149..19291)
                     /locus_tag="PC429_001594"
     CDS             complement(18149..19291)
                     /locus_tag="PC429_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF3847106.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
     gene            complement(19432..20298)
                     /locus_tag="PC429_001595"
     CDS             complement(19432..20298)
                     /locus_tag="PC429_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF3847107.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(20632..22179)
                     /locus_tag="PC429_001596"
     CDS             complement(20632..22179)
                     /locus_tag="PC429_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF3847108.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            complement(22172..23308)
                     /locus_tag="PC429_001597"
     CDS             complement(22172..23308)
                     /locus_tag="PC429_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847109.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(23571..24668)
                     /gene="dinB"
                     /locus_tag="PC429_001598"
     CDS             complement(23571..24668)
                     /gene="dinB"
                     /locus_tag="PC429_001598"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF3847110.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(25058..26563)
                     /locus_tag="PC429_001599"
     CDS             complement(25058..26563)
                     /locus_tag="PC429_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF3847111.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
BASE COUNT         8685 a         4840 c         2777 g        10425 t
ORIGIN      
        1 taaaacctta taaatataca aatacaatta tatgaatttt catcctcaat tatagcatag
       61 aatatgacta tctatatcct aaaattatcc atttctaaaa aaaaggtaac tattattaag
      121 aatatcaact tatagaattt atattccaca tacgattttc aatcaaaaaa acaagggcta
      181 aaaagtgaat tgtgcacttt ttagcccttt attaattatt catttatttc atgtaagaat
      241 agaccacatc ttactttagg ttcaaaccat gttgacttag ctggcattat cttatttgca
      301 tctgctactc ttattaaatc ttcaatacta gtaggataca ttgcaaatgc tactttcata
      361 tcttcattaa ctcttttttc taattcttca agacctctaa ttccaccgat aaaatcaatt
      421 cttttatcta ctctaggatt atcaattcct aaaatagcat caataacata gtcttgtaat
      481 atagaaatgt ctaatgagtc aacagggtct tctatttcta ataaattttt atttgctttc
      541 attttatacc acttatcacc taggtacatt ccgaaagttc cttttttgtc aggtttatat
      601 ttttcttttc cttctatttc ttctagttca aaattttctt ttattttatt tataaattct
      661 tcctcgctta aaccatttaa gtctttgata accctattat aatccaaaac cattaaattc
      721 tcatctggtg caatcattgc tatgaagtaa ttaaattctt cttttccagt ataatttgga
      781 tttttagctc tcatttcttt ggctacattt tctgctgaag ctgtcctatg atgaccatct
      841 gcaatgtata gataatctaa tttttcaaat ttatctacta ggttatctat tatatcatca
      901 ttatcaatct tccatactgt atgctctatc ttgtcatctg ttatgaaatc atataaaggt
      961 tcattattat ccataaaatc attgattata tcatctatca ccctttgatg tttgtaagtt
     1021 acaagtatag ttcccgtatt agcatcacag tattttatgt gtttagttct atcaatttcc
     1081 ttttctggtc gagtgtactc atgttttttt attgttccat tcaagctttc atcaactgat
     1141 atacatgcta ctataccaac ttgagctcta ccatccatga tttgtttata tatatatata
     1201 cactctttgt catcttttac tagaatttct tgttttcgaa actcatctaa attttctcgc
     1261 gcttttaaat aaactttttc atcatataaa tctatatttt catctaaatc aatttctgat
     1321 ttatctatat gcaaaaatga atatggattc ccttctgcaa ttcttctagc ttctttacta
     1381 ttcataacat cgtaaggaag tgctgctact ttatctacta tatatttatt tggtctaatc
     1441 gccttaaatg gtttaatcgt tgccattact aatttcctcc ctaattatac aaaatatttt
     1501 ataatgctac ccctattaga ttgttgaagt caagaaagaa atcttgaatc acatttacta
     1561 tttcgtctcc tattctttct tgagcttcta ctgtagatgc cccaatatga ggtgtaggag
     1621 aaactcttgg atgatttact aagtctaaat ttaatgtagg ttcttgttca taaacatcta
     1681 tagcagctgc tgataattta ccttcatcta atgcttttag taaatctttt tcatcaacta
     1741 aaccgcctct tgcacagttt attaaataag cccctttctt catcttattt atttcttttt
     1801 cagttattac tgccccctta ttcttatcaa atggtatgtg taaagataag aaatctgcat
     1861 tttctaaaac ttcttccata tcacagaatt tataattgtt aaagccttgc gcctcaccca
     1921 ttatgtcagt atatataaca ttcataccta ataattctgc tctctttgca acttcttttg
     1981 caattcttcc aaaacctata agtccaagtg tctttccatt aatttctgta cctttatact
     2041 ttttcttctc ccattttcca tctctcatag ttacatttgc agtatttatg aatcttgcaa
     2101 gaacaaataa ttgacctatt gtaagctctg caacagatat actgcttgca tttggagtat
     2161 tcataacttt tatgccatta gcttgagcat atttaacatc tatattatcc agacctactc
     2221 cacctcttac tatcaacttc aactttttac cctctaatgc tttatctatt acatctttag
     2281 taactttagt agctgaacgt actacaagta catcaacatc tttaagttta tctcctaaaa
     2341 catctttttc ataaaactgc tctatcacat taaaatctaa ctctctcaat tttctcgctg
     2401 cttctttctc tattccatca gtaactaata tattatacat aacaaactcc tccaattttt
     2461 attcacttaa tttttccaat ttttattttt taaagtattt tcatcaataa aaaactttac
     2521 ttaatttaac tttctgttta tataattttc aaaatatttt tttattaaag tcatatcaaa
     2581 ttttcctatt gtttgttaaa aatttaatat gtaattttta taaaatatta tttattatat
     2641 attctaaatt tatcttaaaa catacatatt tttacaatat tatttattaa aacctaatat
     2701 atcatcaata ttatctaata attcttgtac atcttcgaca gtatagtcac ccatatgtga
     2761 aattctaaaa gttttttctt ttaaatctcc atagccattt gctatttgga atcctcgttc
     2821 ttgtaatttt gaatttaaat ttgatacact aataccttga gtattttcta taacagtaag
     2881 tgtattggat aagtgatttt cattggtaaa aatttggaag tgttttttag cccagtttct
     2941 aactaagttt gccatgtctt catgtctgtt gaatctgtta tctaggcctt catctaatat
     3001 attatctaat tggaaatcta aagcgaacat gtgagaaaga gatggtgttg aagggtattg
     3061 gtagtttttc ttgattaagt attcatacat tgctaacaaa tcaagatata ctcctctaaa
     3121 aggtacttgc ttagctctat cgattgcttt ttgagaaaat gtgcaaatag acattcctgg
     3181 tggtaatccc agtgcttttt gtactgatgt tatacaaata tctattccaa tcttatcaac
     3241 ctcgactttt attccaccgg ctgaacttac agtatctaca cagtagatta catcttcata
     3301 ctttttaaca acttctccaa tttcttctat tggatttctt attccagttg aagtttcatt
     3361 gtgagttaca gttattaaat cgtattctcc ggtagaaagc accttatcaa ccatttctgg
     3421 cgttgttgcc tctcctaatt caactttaaa tatatccgct gggacattat tagccactgc
     3481 cattttgtac catctatcac caaaggatcc acaggaaaat actgctgcct ttttggatgt
     3541 acaggaacga atggagcctt ccattaaacc agttccggat gacgtggata aaagtatcgt
     3601 gttgtttgta tagaatagtt tttgcatctt ttcacttata ctcttttgga gcatagatgc
     3661 atcttttcct ctatgtccta tcattggtgt agccatcttt tgcaagactt cttctttaac
     3721 atcaatagga ccaggaataa ataatttctt actcattata attcccccct ttttagtttg
     3781 taatatggat agcgttttta ctgtccttat ttttaataac tataatatca agctgaatta
     3841 actatgtcaa cataaaattt attttttata tataatttat tttatatatt ttatgaaaac
     3901 attcattttc taccttttca taagtctgaa ttttctgaat tattctctca aaaaaaatat
     3961 actttttatc tatataaatt aattttttta gtatgtaaat ttgacgctat tacgataaat
     4021 ctttttagta taactaattt atttttatct tgtattttta ttgtattttt aaattttatt
     4081 ttttctaaat ataaaattta tgttttaaaa tgtaaaattt ttatttaatc aaagttcaat
     4141 atattccact ttttctttcg attcttcaaa aaaccattct tcattagaaa aatcaccttc
     4201 tgcaatttgt ccaataggca ttttcatata ctttccagtt gctgtcactg ctatatctcc
     4261 atttggtaga attatttctc ctgtaccttc aaataatttt ctgttttctt tagttattct
     4321 tccaacaact tttatagtag catctgttgg tactggtttc ttatatttca actctaaaga
     4381 tactgttact ccccatattt ggtcatcatt tatacttaca gctcttccaa tagtctcatc
     4441 caatattgca gcagacatac ctccatgaac tctcccagga taactttgat gccaatcctt
     4501 tgtattacaa attgatacaa gctctccatt ttcaagttca taaaaatctg ctttaagtcc
     4561 tagctcattt tgtgttccac aaactaaaca agatttacta ctattttgct tcttcaaaac
     4621 tttatacttt gccaaaaaat ccatctccta accgacatta gtagtcgctt aatattaaat
     4681 aattctaaaa tatattatat aatattaata aaatccctgc aaacttctaa taataaaaat
     4741 aggctacttc aatatgataa gtttaattta aagtattatt ttccaaaaaa taactttatc
     4801 aaaatagaaa ctcaaagtag atataagcct ctttttcgtt attaaaatag taaaaatata
     4861 ttttacttaa tataaaaaag agtgtctcat taactaataa agtttatttt gagacacccc
     4921 tcataaaaca aattatacta caatagattt ttactaattc tacaagttat atatttaaat
     4981 tatattattc aaattaaact ataattctaa atttcattta ctattaattc gcccattgct
     5041 cttgttccta caacatttcc cacaccatta tagatatcaa tagttctata acctttattg
     5101 agtacacttt gtacagcatc ttcaatacac ttagcttctt cttctaagtt aaaactatgt
     5161 cttaacatca tgcttacaga tagaatagtc gcaattggat ttacaatatc ttttccagct
     5221 atatcaggtg cacttccatg tattggttca tacataccaa aactatcttc tctaagtgat
     5281 gctgatggta acatacctat agaacccgta atcatagatg cttcatcaga tattatatct
     5341 ccaaatatat tattagtcac tattacatca aattggctag ggtcttttac aacttgcata
     5401 gcagcattat caacatacat atgtgacagt tctacatctt caaactcttt agctaagtct
     5461 tccattatac ttctccaaag tcttgaactc tcaagcacat ttgccttatc tacacttata
     5521 agttttttat ttctatttct ggctatttca aaagctctct taccaattct tcttatttca
     5581 ttttcgttat atatttctac atcataagca ttttcgatac catcaatgat ttttcttcca
     5641 cgttctccaa aataaatgcc ccctgttaat tcccttacaa caacaaaatc aatgcctttc
     5701 tcaactatat ccgttctaag tggtgaagct tcttttatac tttcatacat tgtagctggt
     5761 cttaaatttg caaataaacc caatgactct cttaatttta aaagaccaga ctctggtctt
     5821 ttatctccct tacatttatc ccactttggt cctccaacag caccaagcaa tacagcttcg
     5881 ttttttcgac aagtttcaac agttttatta ggtagaggtt ctccagtttc atcaatcgcg
     5941 catcctccag ctaatacata ttcacaatcg aatctatgat taaatttaca acaaatttta
     6001 tttagaacct ttataccttc atctataatt tccggtccaa caccatcacc ttttatgact
     6061 gctatattac aattcatact tcactacccc ctatttgctt ttattgagtt aattaagcct
     6121 tcatttaata taatattttg cataaactca ggaaatggtt ctgctttata ttcttttcct
     6181 tttgttatat tttttatcac acctgtagaa aaatctactt ctatattatc cccttcatct
     6241 atgttattgg cagcttcttc acattcaagt ataggaagcc ctatatttat agagtttcta
     6301 aaaaatattc tagcaaaagt agaagcaatc acacaggata ctccactttc ttttattaca
     6361 attggtgcat gttctcttga tgaaccacaa ccaaagtttt tagttgcaac tataatatct
     6421 ccttttttta ccttagaaat aaatttatca tcaatatcct ccatacaatg cgttgctagt
     6481 tctttataat cagctatatt taaatatcta gctggtatta ttacatctgt atctatatta
     6541 tcaccgaatt taaaaacact tccattggca atcataaatt ttacctccct taaattattt
     6601 ctgatggttt tgatattttt ccaattatag ctgatgctgc tgctacagct gggcttgcaa
     6661 gatacacctc tgaatcaaca tgccccattc ttcctacgaa attcctattt gttgttgaaa
     6721 tggatttttc tcctgctgca agtataccca tatgtccccc tagacatgga ccacaagttg
     6781 gtgtagaaac tgctgctcct gcctctataa aagtggtaat ataccctttt tcgatagctt
     6841 caagatatac tttttgagtt ccagggaata tcataactct aactccatct gctacttttt
     6901 tgcctttcat tatctcagct actacttcaa ggtctgatat tcttccattt gtacaagagc
     6961 ctattacaac ttggtctatc tttatatcct tatctatctc atctactgtt ctagtatttt
     7021 caggaagatg aggaaaggct actgtttctt ttagtttggc taaatctatt tcgtatactt
     7081 catcatagac tgcatcttca tctgcttcat atatatttac atcttttgtc atagttgaac
     7141 acttatgtga atttatatat tccatagtct tatcgtctac tggaaaaatt ccattttttg
     7201 ctcctgcttc tattgccata ttacaaatag taaatctatc atccatagat aagtattcta
     7261 ctccatctcc acaaaattcc attgacttgt aaagtgctcc atcaacacct atctccccta
     7321 ttatatgaag tattatatct ttaccactta tccatttatt tggtttattc ttcaaaacaa
     7381 attttattgc tccaggaacc ttaaaccaac attttcctgt agccatacct gctcccatat
     7441 cagttgaacc tacaccagta gaaaatgctc ctaatgctcc atatgtgcaa gtatgagagt
     7501 cagcacctat aattacatct ccagcagtta ctataccttt ttcaggaaga agaacatgct
     7561 ctattcccat atctcctaca tcaaaaaaat ttacaactcc ttttgatttt gcgaaacttc
     7621 ttgtaaattt acattgttct gcactcttta tatctttatt aggtgtaaag tggtctaaaa
     7681 ccatcactac tttagtctta tcataaacat cttcaattcc taatttttca aattctctaa
     7741 tggctactgg tgatgttata tcattaccta aaactacatc taaatctgct tcgattaatt
     7801 gccctttctt aacttcattt aatttagcat gtttggctaa tattttctgc gtcatagtca
     7861 tccccatttt ttatttcctc ctcaatataa cttttattta ttgcattcat aagtgcatta
     7921 atagctgctg tatttatgtt ttccgatata cttacaccaa aatagacttc attattgtgc
     7981 tctattccta cataagctac ccctcttgaa tgtgaccctt cctctaatgc atgttcccag
     8041 taatatctaa atttataatc tttcatgcca cattgcttta gtgcattatt aaatgaatct
     8101 acaggtccat ttcctatacc aacaatcctt tgttcatttc ccatataact tattcttgca
     8161 gtcatctcaa tcatattagt atcattagaa tcatcatttt catagtttaa ttcatccatt
     8221 gattttattt tatacttctt aactttataa gggctctcaa cttcaatata ttctttctta
     8281 aacaaattaa atattgcttc actactaagt tcttccccaa ttctatcaga ttccattttg
     8341 actacatttc caaaatcaga atgcatattt ttaggtatca taaatccata atctgtttcc
     8401 attatataaa cagctcctcc ttttccagat tggctattta tccttataat ttctttgtat
     8461 tctcttccaa tatcatgagg gtctattggt aaatatggaa cttcccagta atcattatct
     8521 ctgtttttca ttgctatcat gccttttcta atagcatctt gatgtgaacc tgaaaaagca
     8581 caatgtacaa gattacctgc atatggatgt ctatcatgta ccataagttt tgtacagtct
     8641 tcatatatac ctattatctt atctatattg ctaaaatcta gctctgggtc tattccttgt
     8701 gtataaagat taagtcccac atttactata tccatattcc cagttctttc accatttcca
     8761 aatagtgttc cttcaagtct atcggcacca gccaataatc caagttcact tgcagccgta
     8821 caagttcctc tatcattgtg agtatgcaaa cttagtataa tagaatccct acaacttata
     8881 tttttacaaa accattctat ttggtctgca tatacgtttg gtgttgccat ttcaactgtt
     8941 gatggaagat ttattattac ttttttttga ggagttggtt tccaaacatc tatcactgcc
     9001 tcacatattt caagtgcata atccatctct gttcctgtaa aactctctgg tgaatattca
     9061 aaagtaaatt ctgtttctgg atattttttt acttcttcat taaataattt tgctccttca
     9121 actgctatat ctattatttc ttgtttactc atattaaaaa ccacatctct ttgaagaaca
     9181 gatgtagagt tatataaatg tataattgct tttttacatc cccttattga ttcaaatgtt
     9241 ttttctataa gatgagccct agattgtgta agtacttgta tagttacatc atctggaatc
     9301 atattctcat ctattaattt tcttaaaaat gtatactctg tatttgatgc tgatggaaat
     9361 cctacctcta tttctttaaa tcctgtatca ataagcattt tgaacattct gactttttca
     9421 tttacactca taggtgttgg cagtgactga tttccgtctc ttaaatcgac actacaccaa
     9481 attggagcct taaatatttc attatctggc catttacgat ttactatttg cattttatca
     9541 tattttttat actttccaca cttcatgtta tttccccctt tttctgtttt ttgtattcag
     9601 tatacctcat atcgacgcaa tcctgcacaa ttcataagga taaatgtcaa ttttatcatt
     9661 agaataaaaa aaacctcatc tctaataaaa ttagagacga ggttttgctc gcggtaccac
     9721 tctatttagc tttctaaaaa gctcccttag taatagccaa tacaataatt ataatgacta
     9781 tatttcctat aacgtggaat tactccgatt attctaagat ttaaatctta gaaaacagct
     9841 cctagacaag ttcagaacta tcaactacta aatcacacca tcctttagct ctctgaaagt
     9901 ttcattgttc ttactacttc tattcatcac ttttttaatc atttaaattt tctaaatatt
     9961 ttgatttaat agttattata tacacattaa aatgtattgt caacacaaaa aataattatc
    10021 tcaatttttt atttttgata agcaattcta taaaacatgc taactattga aaacagtatg
    10081 atattatgcc ttaaactttt tcatcttttc atctatgata ttcaataatt ttttttcatc
    10141 ttccaaactc aatgccctta atctaatttt ataattagat tcgttagtgt atatatttaa
    10201 tatatttttt actacttcta ttttttctat tatcttataa ttaaactttt gactttgttt
    10261 agattttata ctgaatatac attgattatt tattaatatt gaatgattat cgagagtaat
    10321 atatactttt ccctcatatt tatttgaaaa atcacgacct tgtttaaata gaaaaactgc
    10381 tgttattata cataaaatat ataatattgt cgttgaatat ataaaaattg tagctaaaat
    10441 tataagtgct aaaaccatat atatagtcat tcctttttta aaataaccat tgtcataata
    10501 attcatatag ataaacttat ttttactcaa attttttcac tcccaagatt ctaaaaattt
    10561 atatcaaaaa attctataat ttcatcttca tgataattta tgactaattc atctggaaaa
    10621 tctactgctt ctaaaacttt ttctgcattt tcaaattctc ctatagaata acatatatga
    10681 gagtcagtac caagaattac tcttgcacca tatgttttac acaatgtcaa catatgactt
    10741 acattttccc aagtacccgt tctatgagag tttgaactta atgatgaatt atttatttct
    10801 aaaaggatat ttttatcttt agcttttttt actataggtt cataatctaa aggataccta
    10861 ctgtcatctg gatgtccaat tatttttacc ttaggtttat ccataacttt taaaatagca
    10921 tttgtatttt cttcttttgt accagaagct atacaaggtc tatgcaaact tgcgattata
    10981 taatccaaat gttgcaacat atcttcttga acatctaaat taccattata atccattata
    11041 tttccttcta ttcctctaag aatctttacg ccttgcactt cacgtggaag caaatgcaaa
    11101 ttataaaaat agaatggatg tggtcctcct ggcatattag gtccatggtc tgatattcct
    11161 aagtatttta tattcttctt actcgcctct tcgacatttt ccttgaaagt actatgtgca
    11221 tgtccactta ttagagtgtg acaatgtaca tccataatag actttttcat taaatttcct
    11281 cctaatattt tacataaatt cgtgaaaata gctaaagggc tatttcaaaa ctaaagctgt
    11341 cataatagac agtgtaacgt tttaaaacag ccccattttc aattatttta cacttctaaa
    11401 tctttaaatt tatctttaaa taattctcca atacttccta aagattcctc tacttctagg
    11461 tattctgtta tatcctcttc tggctctcta gttgcttctt ttattgaaac caacattctc
    11521 ttagtctttg gtttaaaatc aagaatctta actttaacct catctccaat attaacaaca
    11581 ttagtaactt tagccactct agtatcactc aattcagata ttggtaaata cgcatctaca
    11641 ccttctttaa tttgaacaaa agcccctttt tctattattc taagaacttt agcattgaca
    11701 acatcatcaa tttgtacttc ttttgctatt aaatcccaag ggtcattatt tatgtctttt
    11761 aatgccaagg caattttttt agtctcttta tttacatcca acacataaac ttctacttct
    11821 tgtccctctg aaacaacatc ttcaacactt tcaactcttg tccaagctaa attatttata
    11881 tgtgctaatc cttcaatccc accaatatcc acaaatgcac cataaggcat tatctttgtc
    11941 accttaccat ctcttttttg tccaacttca agagaattaa ataagtcttc tttagctgct
    12001 ttgatacgag ctttttcttc ttctctttct tgcttaattc ttgctctttc agcttctttt
    12061 tctgccttaa tttgagctct tctttcttct ctctctttat ttatacgctc ttgtaataca
    12121 tctctatgag atgctacaag tctatttttt ctaacatcca attcttttat ataaacttct
    12181 aagttaagac caacaaattt agatgtatct gtaataaatt ttgtatcaag ttgagaaatt
    12241 ggtataaaaa atgtatatgt attgtaatta gcaaatacac ctttatctat attcttttca
    12301 acatttacag ttattattct atgttcatca taagcctttt gtaactcggc aaaatcattt
    12361 cttttgtcaa gttgtaattt agataaagtt attgtaccat ctttttgaga tacttttgtt
    12421 attacagcag ttatttcatc acctatgtgg tatatatctt ctatatattg atttttttct
    12481 atgttcaatt cacttatagg tattattcca tcaaacccat agtctaatcc taattgtact
    12541 tcatcatttc tcactgcagt tatttttcca gttgtcaatt ctccaacttt cactttgctg
    12601 aatacttgtt cttgctggtc aagtaattct tgcattgtta aatcattttc cattataaac
    12661 atcctttcct cttattgatt atcatatctg taaatattat accattaata ttgcaaatac
    12721 ttacattttt cttactataa tttttaaaat aaattcaact tcatatattt atacattata
    12781 gaaaatatct tattaaatta aaatacaaat tctaattttt atctaataaa atattttcat
    12841 aaaaaattta tattgtatat cttattcttt tattgcttta ggttttgata gaaaacattt
    12901 ataaaatata aagaaagaaa ttgctgttat agtagcagct gttatatctg caactggctc
    12961 tgctaaaatt actgcaaaaa gtttttcatt aaagaatgtt ggaagtataa atattaaagg
    13021 tattaaaagt acaatctttc taagtagagc taaaaccaaa gatattgttg cttttccaag
    13081 tgctaaaaat gtctgttgac aagctatttg aatcccaaac ataaaagcac cagcaaaata
    13141 tatttttata ctccaactag tcatactaag taactgcgga ttcttattaa atatactaac
    13201 aaaaaatgca ggaaatatca ttaataaaag ccataaaata gtagaatatg taaaacatga
    13261 caacaaacac aacttaaatg tcttttttac tctatcaagt tgacctgaac cataattata
    13321 acttattatt ggttgtgctc cttgtgtaac ccccatatta ggaagcataa gaatttgcat
    13381 tatactgctc attatagcca ttgaacctac tgctaaatct cctccataca tttgtaattt
    13441 actatttaag gctataagta ctaaactttc tgtagattgc attataaatg gagagattcc
    13501 caaaccaagc actggtaaga tgatacttgc ttttggtatc atatacttct tttttatttt
    13561 aagtatactt ttctttccaa agagaaacat aagtacccaa atagcagaaa cgccttgtgc
    13621 aattatagta gctaatgcag ctccttttac acccatatta aagccaaata taaaaatcgg
    13681 gtcaagtatt atgtttatta aagcacctat agcaactgtc atcattccag ttgtagcaaa
    13741 cccttgggta tttataaaag tattcatacc cattgaaatc tgaacaaaca ctgtacctat
    13801 taaataaata tttaaatagt ccattgaata tccaattgta gattcacttg ctccaaacat
    13861 ccaaagtaaa ggctcttttc caatcttaaa acctatagtc aaaagtattg ctaaaatcac
    13921 cagtacagaa aaactgttac tcattatttt ctctgcctca tcattgtttt gctcacccat
    13981 ttttatagca gctaatggac ctcctcccat accgattaaa taactacatg cagttattat
    14041 aattattatt ggaaatgcca ctccaacacc agccatagca acttctccat tttccattct
    14101 accaataaaa atcctatcga ctatgttgta taaaacattt acaatttgtg cgataatagc
    14161 aggtgtagca agtttaaata ataatttacc cacactttca ctacctaagt tagtgtttga
    14221 attttccatt ttaaaacctc ctcaatccaa taattaaaat gtatcactaa aattttattc
    14281 tatataaaaa tttcatttta ttagcagaca aatttagagt actttcccta caagtaatac
    14341 tatactatat ttcttttgcc atagtaaaca ttttagaact aactgaagtt aaatacttta
    14401 tctcattttt tatttattat taaaaatttt tcacacatac tcataaaaaa actatccaag
    14461 tatattactt agatagtttt atattatatg taaagaaaga gctgaaacta taacccctga
    14521 taacaaattg ccaagaaata ctcccataaa agcatctttt attctcattt ttaatatgga
    14581 agctatagca gatgcagtcc acgttcctgt agttggcagt ggtataccta caaatagtat
    14641 tataccaatt atcgaaacac tttttaattt cttcattctt gagtttattt ttctatcaat
    14701 tacatcagca atccctttaa atagtttctt ccctcttaaa aattgtaata catgtctgaa
    14761 tgtcaatatc aagggaatcg ataccaatgt agaacctatt acactaaata cataaaccca
    14821 tataggattt aagcccattg caatacctat aggaatggct cccctcaatt ctgttacagg
    14881 aaccatagat aaaattaata tgtgtaaata tcccaaacta ttccctccta ttctaagtga
    14941 cattaagtta tgaatttatt tgctctataa cctcaccatc ctttttacta actaaaaatg
    15001 catttttagt caatgaaagc attggtaaat cactcttaga gtctgaatat gcatatgaat
    15061 tttcataatc aatctctatc ccaagtttac ctaatacagc ctttattctt tttacttttt
    15121 caattccctt acagttttct ccaatgaatt tatttttata tcttgaattt tcataaaaaa
    15181 actccgtgcc tataacctcg tctgcaagac cataatactt aaaatatttc atatatgcat
    15241 atggagatgc agttatcata attataacat tatcttcttc tttatttttg taaataacct
    15301 ttttcataga ttcgttaatt tttttcatta tgcaatcatc aaaaaacttt tttaaatctt
    15361 cttcactaaa atctactatt ggataaaaga ttgcctcttt tgccttcttc aatggtatta
    15421 ttctaattaa atacataaat agaataaaaa taatgtatgg taactttata atagttttaa
    15481 aggttttatt ttttaaagag taaataaaaa aatctatcat gctatcagta tttataacag
    15541 tataatctac atcaagtaat aatacacttt ttttcataag tatttcccca tcctatcact
    15601 aaaaatatca ctaaaaaatc aatattatta aacatgtaat tatccataag attacgttta
    15661 tttttaaagc attatctttt aagacaatat cagtaggatt attttcattt gtagtgttaa
    15721 ttatataatt atatctaagt acaccataaa ctacaaatat tgttgtaaat atcatatgtg
    15781 gcttttctgg attacttgta gaatacaatg cataacatac aattgtagaa cttaatataa
    15841 ctatcatcat ttgatttaga ttttctatgt cataatcttc caatattttt ctatgttcaa
    15901 ctgcatcatc tcttagagtt tctatttctt ttttacgttt tccaagagct aagtacagag
    15961 ataagaaaaa tgtacataaa attatccaat ttgataaact aacacttatg gctacacatc
    16021 ctgcataaac cctaagtata aaaccaagag atattgaaaa cacatctaat agtattatat
    16081 tctttagttt aagagaatat aatatattgt ttatcaaata gagactgaga attatagaaa
    16141 gattaaaact aacaactgtt gataaacaga ctgaaattaa agctaatact acaaatagta
    16201 tattagcact ttttttgctt attcttccac ttgccactgg tctattacat tttttaggat
    16261 gttttttatc tttatccatg tctatagcat cattcagtgc atatactgat gatgacatta
    16321 gacaaaaaca cacaaacgca ataaacgtat ttaaaaataa atttatattt agtatgttgt
    16381 ttgaaaaaat tatagctgaa aatacaaaga aattctttat atattgcttt attctcatta
    16441 actttatata atctgaaatt ttagtaagaa gccttatact tgtattggtc tgtttcataa
    16501 acttactacc ctttcaatat tattttttaa acaggatatg attttatccc tatgtttaaa
    16561 atttctataa atccattgac attactgatt actatagtaa ccaataacag cgatagaaat
    16621 atattataca tattcttttc attttttcta agcgctttcg taaagtaagc caataaaatt
    16681 ataactgcaa aactaaaatt tatagccata atacttgaat tgactagatt atatcctact
    16741 ataccatgta aagttagatt gaatataatt attcccatac atagcttagg aactcttttt
    16801 tctgaatttt caataactga taaaacacaa aaacataaag ctaataatgt aagaattact
    16861 ggtattgtag caacttctcc attttgcaaa aataaccctt ttttaatact tgctccagga
    16921 aaataaaaaa ccgaatacaa taatctctct ataatcattg caatcttttc caaaattggt
    16981 tctccactta taaatttcgt atcatactgt attacatttc caaggttcat aaaactctca
    17041 taagactttg ttgaaactac acaaatatat gccattagga taaatgtaat taaatgtttt
    17101 aacatttttc ctatttttaa tttaaactca aatatcatat taaaaaaata caaatatata
    17161 ttactaatag ttattccaaa tagaggaata gctgctaatt ctactaaata agaatttttt
    17221 cctttcatct tatttgcaat aactatgaaa aatattaatg aaaattggct atatataaat
    17281 ctctctacat ttaaagtcat aaataactgt ggaaaagaaa atatcattat catagtaagt
    17341 aaaattaaag ttaatctctg catttttatc tttttaatat gattaaagac taaagttatt
    17401 gacatagcac tagttattat ttgtagacaa attagtccaa acccataggc actattaaaa
    17461 tctgttgtcc caggatttgc tattgaagta caaaataggt atattggata tgcaaaaatt
    17521 gatataattg ctgaaaacaa tatgtgctta ctattgtctt gacttatagc aaatacattt
    17581 aaattgaata aaactcctgt atctgtatct aacaatacat cataagtatt gtttaaatca
    17641 taaaatatag tagtcttaga aaaaaatatc ttagttatta ttaagtaagt tattacaact
    17701 aatataaaca aaaacatctc gtttttatat tttaatattt tactcatcaa tatatcacca
    17761 tccattcatt atataggatt tgctgacacc tacaatttat tccttatatt taatagccat
    17821 cttcataatg ttcttacttg ttttattgta attgaataac tctttattta caaccacaat
    17881 atttacttta ttgatgttat aatcagtcaa gtattgttta tataacatag attagtgttg
    17941 tataaaatga gaatttgtaa gcaatattaa tgtaaaatat ttgaaaaact ttatataaaa
    18001 atatacaatg ttgcttatgg aattaaacta taacaaatta aagcataatt taaggagaca
    18061 aaattataat ctgtctcctt aaatattaaa actatatttt aaaacattac taaatactat
    18121 acaaatttca aatatagtat aaaattaatt agactttaac actattagct ccatcttctt
    18181 cccatttttt aatttctttt aatctatcta ttataggtaa atgctgagta cattcaactt
    18241 cacattttcc acaagatata cattctttag cttttgcctt actattcatt gtgtaattcc
    18301 aatgtcctaa gccataaact aactttgtca tttcttcatc actcttttta aacatctgct
    18361 tttcattata aaaaagcata taggctgggg tattaatacc ttttggacat actttacaat
    18421 aaccacaacc tgtacatatt tcgttcatat tcttatttaa tctagtctca atatcatgta
    18481 tatcctcatc tgaataaatt ctattttcgt ctgcaatttt acatgcatca tctatatctt
    18541 gttttttatt aaaccctatc aaagatatac ttatttgtga aattcctatg ttgaatctta
    18601 aagctgattc tatagtactt tcgccatctt ttgatagaaa agaaagttct ttattatact
    18661 ttggaatagt tccaccactt aatgggttca ttgccacaac accatatcca ttatcaacag
    18721 catatttggc accttttagc ctatatggaa agtttaatat atttattccc atagttacac
    18781 cttcaaactt attttcactt agaatatcta taacctcatc tcctggttga tgagatgaaa
    18841 atactatatg gtctatcaat ccttcttctt tacatttcaa taatccttca tattgacctc
    18901 caggtctcat tgctagttcg taatgttcca tctttctaat attccaaaca tgataaaaat
    18961 ttattttaga tacgcctaat cgttctattg atttttttac agcctctata gctttttctg
    19021 atgtatcaca tgcttttggc ttttctttag ttgatacata aaaatcggtc ttaccttcct
    19081 ttatcatttg tctaaaggct agtccaaata tatcttcact tctgtcatca caataccctg
    19141 gtgctgtatc aaagtaatta ataccttttt catacgcata tttgactaaa tcagcatttt
    19201 cttgattact tttttctaaa tcaaacctta atcctccata gccaactact gaaaccattt
    19261 tccctgtctt tccatattca acgtatctca ttgtaaattc ctccttagat atattaagac
    19321 ttaatttctt tatagaaaat attataccat aaataatttt tataaattat ttatggtata
    19381 atattttttt tataaattat atttttttat aacttgtatt ttataactaa gttaaaaact
    19441 ataaaaaggt tcaatatgga ctattacttg agcattttca ttaatttctt cttgtatttg
    19501 tttctcaatt ttatgtgtta aatcatgaga ttgctctacg cttataaatg ggtctaccat
    19561 tacatgcatg tcaatataaa tagaactttt gctacctcta cttctgatat tgtgaacatc
    19621 ccttacctca ttaaattcaa acacaatttc ttttatataa tcctcatcta cagccactct
    19681 atcgaccaaa attccaatag atggcctaaa tattccataa gccgaataaa ttatgaatgc
    19741 agaaataata ataggaacta tagattctat tattactgga aatcctaact taacaccaac
    19801 taaagtaacc aatacaccta gagacacaaa tatatcactt cttgtatgca atgaatccga
    19861 aatcaaaaca taactattta gtttagtccc aattctatat tcatacatgc atacaaaaat
    19921 atttataaaa agtgtaataa ttaaagcaat taaactttct atagttatag caggtacaac
    19981 tggattagct attcttaaca ctgcactaag aattatctta attgctataa caaacaacat
    20041 accacttata aataaactgg ttataatttc aaacttttta tgtccataag gatgattttt
    20101 atcttttggt ttagatgcaa agaaaatacc tataagcccc actatattag atgctccatc
    20161 tgacaaagaa tgaaatccgt ctgctgtcat actatagctt tttatttgat taccaataat
    20221 gatttttaat aatgctactg caaaattagc aaataaaatt atccacaata cttgcttcac
    20281 tttcttatag ttatccataa caaattcccc ctcccttcga ttcaataaag aaagctatct
    20341 atcaatatca atcaaccaga caataaaatc gtatataagc acatttacta tatttatatt
    20401 caaaattggt tgtacttgtt cattctattt gaaacttaat tataaataaa aatcgtaggt
    20461 atggttttgg tatacataaa aataagagac tgtctcaaaa taaagataat cttctttttt
    20521 attgttgaaa tgataaaata tatttgattt catataaaaa agagtgcctc atgaactaaa
    20581 aagttcattt tgagacactc ccttaaattt ttgatataaa ctattaattt tttatactaa
    20641 gccttcttta actaagaatg aatgagcaac ttcttctggt gatttaccaa gttcatccac
    20701 ttgatagtta agttctctca tagtttcttc atttattttt cctgaaagca tattcataac
    20761 tttttctaac tcaggatatt tcttcaaagt atcttctctt actaatggtg ctgcataata
    20821 tggtacaaag aaatttttat cgtcctctaa tatcttaaga ttaaactttt taataagacc
    20881 atcagttaaa aatgcatcta taacctgaga ttcattatta tctaaagctg tatatcttaa
    20941 acttccattc attgctttta catctttgaa ttttaaacca taatctctag aaagccctac
    21001 aagaccatct tctctatttt caaactccaa agttggtgag aatgtaaatt ctttaccata
    21061 tttagtcaaa tcagaaatag tatttattcc atacttttca gcagtttcag gcatcatagc
    21121 taatgtgtaa gtattattaa atccaagagg gtcaagtaaa gttaactgat tttctttttc
    21181 catagtatct tttacaacat tatatgcttc atcagcatct ttaatcggag catgtttcat
    21241 tacatttacc aaaagtgtac ctgtatagtc aacatacatg tctaactcac cagatttaat
    21301 tgcattaaac gcaacagatg aaccacctag atttaaattc ttcttaactt taagattcgt
    21361 atttttttct actaattcag cataaacatt gccaagtata agttgttctg tataatcttt
    21421 agaaccaata gttattgtat cttttttact tgaaaaacta gaactaaaaa caacgaacaa
    21481 cattgaaaca acaagcacta cagatattac tttaagtgct atataattct tcttaggtgc
    21541 ttttggattt aatccttttg gagtaactgc aacttcaatt ttactaaata aataatcaac
    21601 taacagtgca attatacatg atgggattgc ccctgctaaa atcatattat tatttacagt
    21661 ttgaacccct gaaaatacta aatatcccaa accaccagca cctataaatg ctgcaagagt
    21721 catcaaacca acagctgtaa ctgcagatat tctaactcca cccattatta taggaagcgc
    21781 taaaggaaat tgtattttaa ataatgtctg atttcttgta agaccaatac ctttagctga
    21841 ttctaatact actgggtcaa tacttgttat acctatatac gtatttttta caattggtag
    21901 taatgaataa accactacca taaatatggc tggtttactt cctataccaa gtataggtac
    21961 aagaagaccc aaaagtgcca tagacggaac tgcttggact aagtttacaa atccaagtac
    22021 aaactttcta agagatgaga ttcttgaaat aattattcca agaggaacac caactattat
    22081 agctattaaa atagatgtaa cagttagact catatgttgt attaaaagct ctataatctt
    22141 atctttttgt aatagtataa agttaaaaaa attagccaag aacactcacc tccatttcta
    22201 agaattgttc acttaaaaca gaaagtaaac tacttctagt aataagccct accagtttat
    22261 tttcatcaga aataaccgga atatatccaa ccgaattacg attcattaca tttagtattt
    22321 ctactaaatt atcaccttca tttacatgca aaggctcact tgacataata tcagcaagta
    22381 atattgactt atcatcaata tctttcatat cttttacagt aacaatacct tttaatgttt
    22441 ttgctctatc tataattagt aagctatcaa ccttactcgt tctcataatt tctattcctt
    22501 gagttacagt tctagtagaa ttaacagcaa taggattttt tatcataata tctcttgctt
    22561 ttatatattc tggattatcc caaactctat cttctccgat aaaatctttt acaaagtcat
    22621 ttgcaggctt tctaagtata ttttcgggag tatcatattg tgctattctt ccatccttca
    22681 taatacaaat tttatcagct atctttaaag cctcatccat atcatgagtt acaaatatta
    22741 tagttttttt caattcatct tgaagagaaa ataattgttc ttgtaaagat gttctcgtta
    22801 ttgggtctaa agcactaaaa ggctcatcca tcaatattat ttcagcatca gtagcaatgg
    22861 ctcttgcaac accgattctt tgttgctgtc caccactaag ttctgaagga tacttattca
    22921 aaaattcatc actatcaagt cctaccatct caagtagttg caacgttctt ttttcaattt
    22981 cttcaacgga tttttccttt tttaggcgtg gaattagctc tatattttct tttattgtaa
    23041 gatgagggaa taggcctgtg ttctgtataa catatcctat ttttcttcta agctctattt
    23101 cattttcctg agaaattggt ttgccattaa tatatatctc accagatgta ggttttatta
    23161 atttgtttat aagcttcaaa gtagtagtct ttccacaacc acttgaacca atcaaaacta
    23221 ccaatgtacc tgtttctaca actagagaaa tatcatctaa tataacatta tttcctattt
    23281 tttttgttac atttcttatc tctatcataa tttatcatcc cctttttcga acaagcaacc
    23341 ttcttcaata ttataagttg tttttataat tatatcatac tattttattt atgtaaacaa
    23401 agtacgattt ttaggtcact atttgtctga atattgctac aaatataaag tatttatata
    23461 tagtatataa tacataatgt caaaaaacaa aaaatataaa aatattcata aaataacact
    23521 agcctcaatt atatagtatt aaagctagtg ttatttcatt taaacaaaac tcacatttgg
    23581 atactatcca tgtattcaaa taatgtaata tctttataag tttctttttt atcacttagg
    23641 tttgatattg taactcccaa tagcctaatt tgttttccat taacatcaac atttctcaac
    23701 aaattatcta caccacttct tatatcatta tattcgtcaa tatagtgttc taaactaagg
    23761 cttctagtta tctttgtaaa gtcctcatat ttaattttta atgttaatgt ttttccaaac
    23821 ttctcagagt tttttaatct atgacatact tcttcacaaa gttcatcaag aatgttgcgt
    23881 gtttcttctt catcaatatc caaattatga cttaatgtag tctcagcccc aacagatttt
    23941 ctaaccctat ttggttccac tggtctatcg tctattcctc tagcaaattt atataattca
    24001 taacctcttt ttttgaatat attttcaagt tcaaataaat ttaaacacct taaatcatat
    24061 ccagttttta tacctaaatt tttaagagta tttgaagtaa cttttccaac accaaaaaat
    24121 ttattcacag gtaatttatc taaaaagtcc tgggcatttt cttcggttat tacagttaac
    24181 ccatttggct ttcttaagtc tgatgcaatc ttagcaagaa acttattgta tgacacacca
    24241 gctgaagaag taagaccaac ttctctaaat atatcttttt ttatttgctt tgctatctca
    24301 attgatgaat caatattttt tttatttttt gttacatcta agtaagcttc atctaaagat
    24361 aatggttcaa ctaaatccgt gtatctataa aatatatctc ttatttgctc tgacacactc
    24421 ttataaacat caaatcgagg tcttacaaaa actgcatatg gacacctatt gtaagctaca
    24481 gctgatggca tagctgaatg gataccaaat tttctagctt cataagaaca cgttgcaacc
    24541 acacctcggc tttgtggatt tccaccgaca ataacaggtc ttccttttaa ttttttatta
    24601 tctctttgtt ctatagatgc ataaaaagcg tccatatcta tatgaattat ttttctataa
    24661 tgtttcatta ttatcacctt ttaaagtata aatagattaa ttttatttat attacttttt
    24721 aattatacca aacttatgtt tctttttggt attttttcat tattttcttt atatattcat
    24781 attttccata aataaaacca tctataatcc attaacttca atataattta taaaaaatcc
    24841 ttaaaaacac ttgcaaaaac tatataaaag ctttacaata ggtattccaa ggaactaaat
    24901 ttaaaattcc ttatggataa cagtatatat atttaattta tgggatgttt taaaaacaaa
    24961 agactgtctt atgcaagaca gcctactgat tttattataa ttgtaatgga ctgctcaaac
    25021 tattacttta aaacagccca tattttttta tataatttta actatagtaa agattttgtg
    25081 aaggatattc tgggtcacaa gttacatcta taccaagctt tctaaatgtt tgttcatcat
    25141 ctctatttaa tatagtagta gaatgagctt gacacccttt taataatgct aacttttcca
    25201 tagcaaattg tgcagttgga ttagttacag cacaaatact aagtgcagtt aacacctctt
    25261 ggcaactaag tgaaacattt ctactagcta aagtctttgt ttttaagttt atgataggtt
    25321 ctaatattac aggtgatatt aagtgcatat catcagatat attggctaaa tgtttaatgg
    25381 catttaatac tactgctgca gttccattca ttaaatcaga acccttacca gttaatattg
    25441 taccatcttc taattctaat gcaactacag gacaagtatc attcttgtca gcactttctt
    25501 tcaatttatt actgtagttt ctagcaggta atacaacatt tctatcttct ggctttaagt
    25561 taagctcttc cataatcatt ttcattctat cataagcacc tttatcaact tgtccctttt
    25621 tatactcaca ggcagtttta aagtatcttc ttattatttc ttctatagaa gcttttctta
    25681 ttacttcatc atctattatt ccaaagccaa ctctattaac acccatatca gttggagatt
    25741 ggaaaattga ttcttttcca gttatctttt ctattatttt tttaagcact ggaaataatt
    25801 ccaaatctct attgtagttt actgacattt caccatacgt ttcaaggtgg aatgaatcta
    25861 tcatattcac atcttttaaa tcaactgtag ctgcttcata agctatattc aaaggatgtt
    25921 ttacaggtac attccaaact gggaatgtct caaacttaga atatccaact acattacctc
    25981 ttttattttc atgatacaac tgacttagac aagtaccaag ttttccacta ttaggtcctg
    26041 gtgctgtgac tactactatt ggctttgtag tttctatata ttcatttgct ccatatccct
    26101 catcactaac tatagtctca acatctgacg gatatccctt tgttggataa tgcttataaa
    26161 ctttaattcc tctacgttct aatttattta taaatacagt tgtagcaggt tgtccttcaa
    26221 atctagtaat tactacacta tttacatcta gttcataacc tcttaaatca tctattaatc
    26281 ttaaaacttc catatcataa gtgataccaa agtctcctct tattttgttt ctttctatat
    26341 ctcctgcata gacacaaatt acaacttcaa ctttttcttt catatgttgt aatactttta
    26401 tctttgcatt ttcatcaaat cctggaagta ctctttttgc atgaaggtct cccatcaatt
    26461 ttcctccaaa ctctaaataa agtttgtcaa aattatttac tctttctaag atatatttag
    26521 actgctcttc taaatatttt ttgtggtcaa atcctacttt catcttaaca cctcactaaa
    26581 ttaaatttct tattgataac agtatattta tatatatttt tttagtaaaa catcttataa
    26641 aaaaatgtta atactttttc actcaaagta atattataca attaagatta atttttttca
    26701 agatagtttt atggtaaata agatata
//
LOCUS       DAMKMI010000040        25966 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMKMI010000040 DAMKMI010000000
VERSION     DAMKMI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25966
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.041"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            119..769
                     /locus_tag="PC429_001600"
     CDS             119..769
                     /locus_tag="PC429_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF3847112.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            complement(909..2285)
                     /locus_tag="PC429_001601"
     CDS             complement(909..2285)
                     /locus_tag="PC429_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3847113.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2301..4214)
                     /locus_tag="PC429_001602"
     CDS             complement(2301..4214)
                     /locus_tag="PC429_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3847114.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4350..4982
                     /locus_tag="PC429_001603"
     CDS             4350..4982
                     /locus_tag="PC429_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF3847115.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5121..5660
                     /locus_tag="PC429_001604"
     CDS             5121..5660
                     /locus_tag="PC429_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF3847116.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(5836..6006)
                     /locus_tag="PC429_001605"
     CDS             complement(5836..6006)
                     /locus_tag="PC429_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3847117.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            6291..7358
                     /locus_tag="PC429_001606"
     CDS             6291..7358
                     /locus_tag="PC429_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF3847118.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            7528..9429
                     /gene="cooS"
                     /locus_tag="PC429_001607"
     CDS             7528..9429
                     /gene="cooS"
                     /locus_tag="PC429_001607"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF3847119.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            9422..9895
                     /locus_tag="PC429_001608"
     CDS             9422..9895
                     /locus_tag="PC429_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3847120.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            9870..11096
                     /locus_tag="PC429_001609"
     CDS             9870..11096
                     /locus_tag="PC429_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3847121.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(11235..12422)
                     /locus_tag="PC429_001610"
     CDS             complement(11235..12422)
                     /locus_tag="PC429_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3847122.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            12695..13519
                     /locus_tag="PC429_001611"
     CDS             12695..13519
                     /locus_tag="PC429_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF3847123.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            13648..14913
                     /gene="gluD"
                     /locus_tag="PC429_001612"
     CDS             13648..14913
                     /gene="gluD"
                     /locus_tag="PC429_001612"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF3847124.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            15126..15716
                     /locus_tag="PC429_001613"
     CDS             15126..15716
                     /locus_tag="PC429_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF3847125.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            16046..17179
                     /locus_tag="PC429_001614"
     CDS             16046..17179
                     /locus_tag="PC429_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF3847126.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            18095..19117
                     /locus_tag="PC429_001615"
     CDS             18095..19117
                     /locus_tag="PC429_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3847127.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            19414..20334
                     /gene="pyrB"
                     /locus_tag="PC429_001616"
     CDS             19414..20334
                     /gene="pyrB"
                     /locus_tag="PC429_001616"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF3847128.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            20373..21065
                     /locus_tag="PC429_001617"
     CDS             20373..21065
                     /locus_tag="PC429_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF3847129.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            21058..21960
                     /locus_tag="PC429_001618"
     CDS             21058..21960
                     /locus_tag="PC429_001618"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF3847130.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            22045..22629
                     /locus_tag="PC429_001619"
     CDS             22045..22629
                     /locus_tag="PC429_001619"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF3847131.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            22844..23770
                     /locus_tag="PC429_001620"
     CDS             22844..23770
                     /locus_tag="PC429_001620"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF3847132.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(23822..24712)
                     /locus_tag="PC429_001621"
     CDS             complement(23822..24712)
                     /locus_tag="PC429_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3847133.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            24900..25931
                     /locus_tag="PC429_001622"
     CDS             24900..25931
                     /locus_tag="PC429_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF3847134.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
BASE COUNT        10057 a         2945 c         4361 g         8603 t
ORIGIN      
        1 gtagaatgga tttgtcaagt ttaatttagt tgtaattcta tcttttatta aaaataacat
       61 aaattaaaac atataaataa aatattttat acaatatatt gtaaaggagg taactttgat
      121 gaaagcagaa aaaacaagta tatatccaat tattaaaaat aatttaaaca aaatatcaat
      181 tgttgaatat aaaaagggtc aaagatttgt agcatcagat aaagacttac aagaagtgtt
      241 ctttataata gagggagttg cattagtgga gtgtactact agaggaggaa ataaatttct
      301 tgttgacatt gtacctgaaa atgaatttat aggcaaaatt agttacatat atgaacataa
      361 tttgaaatgt gatatatttg caaagactaa tataaaatta tttcgatttg aaaaaaatgt
      421 atttgaagag ttttatctca aaccagattt tattgcacta tttcacagaa aatgtacaag
      481 aagaatatat gaattatata aaaccagaat ggtaagagaa ctattttctt gcacagaagt
      541 tattgcctat tgtatatcaa ataaacaaga aaataatgtc tgcaatataa aagctattcg
      601 taatttcagt gaaatctatt caattagtag aaaaagtaga taccattctc tgctaaagtt
      661 aactgaaaag gagatagtaa aaaaaatggg taatacatat gaaattttaa actatgatga
      721 attacataaa ttggcatttg aagtaaaaga atttttggaa gatgagtaaa aattgttact
      781 taatcaaaat aaagtataat aatttctgta aaaaatagaa atagtctcac tgaaaatcaa
      841 aaatacttta gttattttca atgagactgt ttagaaatat tgtatactta taatgtatag
      901 tataattatt aatcaatttc ctttactata ttaatagccc attttttaga taatgctcta
      961 ggaacaccaa atataaactt aaccacttct tcactataag tgagcaaaac gagtacaaat
     1021 ataggtgcac ctttaaatgc agctataaaa gttagaggaa tagaagctaa ccacatacat
     1081 cctaattcta aaaatagagc atatttagta tctcctccac ttcttaaaat accaattata
     1141 accaatgtat taaaagattt aagagccatt agtattccca ttataaagaa tatcttagtt
     1201 atatcagaag ctaagctatc agaaacacta aacattttta ataataatgg tatatttaaa
     1261 attaaaataa ttccaagtat taaaccagct gaaaatacaa gtatagaaaa ctttttagca
     1321 tattcaatgg ctcttttaat atggtcagct ccaagttcat ttccaagcat aatagatgca
     1381 ccagatgcaa tacatacagc tgtcattatg aagaaattac ctgtactggt tgctatctgg
     1441 ctagctgcta tagcagaagt accagctctt gcatatgcaa cagaatacag gacagttcca
     1501 attgcccata cagagtcatt tacaaatata ggaaagcttt ttgatgaaaa agattttata
     1561 aaaattttat caatagcttt tagatttttt agaccaaact ttaatatata gtctttttta
     1621 taaaggtaaa cataagtaat cattaaaatt aattcacata ttctagcaat aacagtagct
     1681 agagcagcac cttttacacc caatgcagga agaccgaagt taccaaatat aaatccataa
     1741 tttaaaataa catttgttac aagtgcaaat gcactacaaa tcatacctaa cttaggattt
     1801 ctaacacctc ttgaaccagt actaaataca gtacttacag caattaatgg ataactaaat
     1861 actgcaatac taaagtaatc tatacaaagt tttaccacct cagaatcata cgaaaatatg
     1921 tgtataattg gctttggtgt tagtaaagca ggaataacaa atacaaaact taaaacaatt
     1981 gccaatacac aagtaagacc tgtgactttt cttatattac tcacatcctt ctttccataa
     2041 aactgtgaaa taaaaactcc agcacctcct gtgataccag ataaggacat attaaaaaga
     2101 aagaaaaatt ggtttgcaac tccaactgaa gctacagatg tttcccccaa actacttatc
     2161 ataattgtat ctattacatt tacagaagta gaaattaagt tctgtatgat aataggtata
     2221 cataaagata gaagtatttt ataaaatctt ttattttcgt cttttaaaga aaatagttct
     2281 tgcaaataat cctctccttt ttacataaat tcttcccact catcatatag agaggcaagg
     2341 ttattttcta aattaatttt ttcttggcta acttctttag ctttatctgg attagagtat
     2401 acttcttctt gacataaaag tatatctaat ttttcaattt caatctctaa cttttcaatt
     2461 tctttttcta tattttgtct ttttattcta tttttctttt ctatttctct ttgttctctc
     2521 tcttttcttt tttcatcttt tatttgagtt ctagtttttt cttctttttc ttctataata
     2581 ctcatctctt ggatttgcct ttttttatct atataataat cataatttcc cagatattca
     2641 gtaattccat tttcatctaa aactaaaatt ttatcaacaa cagtatttag aaagtatcta
     2701 tcatgtgata ttgtaaatat agtcccatca taattagtta aggcctcttc aaggacttcc
     2761 ttagaatcaa tatctaggtg attagtaggc tcatctaata acaataagtt tgcatttgat
     2821 agtataagtt taagtatggc aactctagct ctttctccac cactcaatgt agaaatcttc
     2881 ttaaaaactt cttcatctac aaataaaaat gcccctaaca tatttctcaa agtagtttga
     2941 gtaagatgag tattattttc ccaaatctca tcaattattg tgttatctaa attaagagtt
     3001 ttttgttcct gatggaagta ggaaacatgt acattagttc caaatttaat attgccacta
     3061 agaggttgta gttcattagt tattatttta aataatgtag atttgccaac accatttgca
     3121 cctataagtg caactttttc ccctctatat atatctaaat ccaagtcttt aaaaagtatt
     3181 ctttctccat aacccataga tacatctcta atttgaagaa catcattacc actctggaca
     3241 gaaggcgtaa attgtatacg agcttttttc ctataagctt ctggtttatc taaaacatct
     3301 actttgtcta atgctttttc tttacttctt gcacgcttta ggtgtttttc tctaccataa
     3361 gcttttagtc tttcaattga ttcttcttgc ttttttattt ctttttgttg gtcttcaaat
     3421 ttcttaagct ctagttcttt ttctatttta gataactcaa caaatttaga gtagttacca
     3481 ttatatactt taagcttttt attgtgaact tcaaaaattc tatttacgga ttggtctaag
     3541 aaatatctat catgagatat taacatgaca gtccctttat attgttttaa aaatacttct
     3601 aaccattcta ttgcttcaga gtctaagtgg ttagtaggtt catcaagtaa caatagagtt
     3661 ggtttcttaa gaagtaattt tcctaaaaga actctagttt tttcaccacc agaaagtatg
     3721 cttattggct tatccatatc attgtcacta aatcctaatc cctttagaac tcctttggct
     3781 tcagatttat atccatatcc atttaaatct gaaaataatt ctagtttatg tgaatattca
     3841 tccatgattt tttctataat tggagagttt gtttttgtac tttcttcaga tattttaacc
     3901 tccaattctc ttaaatagga ttccatttct atcaaatttt taaaaacttc tagtacttct
     3961 tccaaaatag tattatccga ataaaaattg gtattttgtt ctaaatatcc gatttcacaa
     4021 tctttagaag tgtaaatatc tccactatca tatccataaa ttcctgaaat tattttaaat
     4081 aaagtagttt ttccagtacc attaacaccg attataccaa ttttatcgcc ttcatttact
     4141 gtaaaactaa tattttctaa tatagaatct ataccaaaac ttttatttaa gttattacat
     4201 gacaaaacaa tcataaattt ctacctcctt gaactatcta aaattataat gttttgatat
     4261 aatataatca aggttatatg tattagtttt gaaaaagaaa tattttattg tgaatcaaac
     4321 aaaaaagagg acacttttag gaggtgaaga tgttgggaaa taaaaatata tcaatggcag
     4381 ttataagaag gctcccaaaa tatcatagat atcttggaga cttattagat agggatatac
     4441 aaagaatatc ttctaaagaa ttgagtgata taatagggtt taccgcttct caaataagac
     4501 aagatttaaa caactttggt ggatttggac aacaaggata tggttataat gtagaagctc
     4561 ttcatactga gataggtaaa attcttgggt tggatcgacc atacaacgca gttcttgtag
     4621 gagcaggtaa cttaggacaa gctatagcca attatgcagg atttagaaaa gctggattcg
     4681 agataaaagc tttatttgat gcaaatccta gaatgatagg tttaaagata agagagtttg
     4741 aagtattaga ttcagatact ttagaagact ttataaaaaa caataatata gatattgctg
     4801 tattatgtat acctaaaaat ggagcacaag aagttattaa tagagttgta aaagctggaa
     4861 tcaaaggtgt atggaatttt gcacctttag atttagaagt tccgaaaggt gttatagttg
     4921 aaaatgtaaa cttaacagaa agtttattta ccttatcgta tttaatgaaa gaaggaaagt
     4981 agacaactta aacatattaa taatgagcag ttgtaaagag tattgagaaa gtcagaagat
     5041 ttaatattaa atcctctggc tttttgaaat attaggataa aaatttaaat aatgtatgga
     5101 aataaacaat attaaggaga gtggtttttg tggaggaatt atggaaaaag gcaattgaat
     5161 ttcatggtca tgaatgtcca gggttagcta taggatttaa agcagcactt gcagcaaaaa
     5221 aatatttagg atgtaaacag tcaggagatg aggaaattgt atgtatttct gaaaatgatg
     5281 cttgtggaat agatggtata caagtagtgc taagttgcac aatgggtaag ggaaatttac
     5341 tacttaggat gactggaaaa tcagtatatc atttctttaa tagagaaaat ggaaaatcta
     5401 taaggatata tgtaaagtca aaaccatttg agatggatag agaaggatac aaaagatatt
     5461 tattagaaag tgattttgaa gaaatatttg agattacaga tactaagtta agacttcctg
     5521 aaaaagcaag aatatttaaa tcccttaaat gtgaagtgtg tggtgaattt gcatctgagc
     5581 aatatataag gattgaagaa ggaaagaaag tatgtcttga ttgttttgat aaatatgaaa
     5641 gattttatga agagtattaa aattataaat tagattaaag ttaaaagtat tagatatgtg
     5701 ctaacagtat tttaagatga attaggtagc aacataataa agtatataaa taatattaat
     5761 tgaatcatac ataattaaaa agaggctgat tagtatttat aaaataaaat cagtctcttt
     5821 atgaataacg aattattatt ctggttgagg tgcatctaca ggacaagctc ctgcacaaga
     5881 accacagtct atacaagaac cagcatctat aacatatgcg tcgtctccag ctgatataca
     5941 gctaacaggg cattcagcct cacaagcacc acagcttata caagcgtcag taattttata
     6001 tgccattttg aaatcctccc ttttatatgt attgtaattc aataacatta taacaaatat
     6061 tattgaatat aacaattaaa ttaattctac aacaaaaact ttattatgat attcttgttt
     6121 taaagtttta caataaagtt taatctgctt atatttttat ttattttaaa gaagtttatt
     6181 ttaaaaataa aattaaattt aaaaagttat gtattgatta tatttaaaaa tgttatactc
     6241 taaaaaagta tattttattt aaaaatttat taatacaaag gagatttact atgaaaaata
     6301 aattagttta catatgtatt atgagtctat tgctatttgt agtaggatgt tctaataatc
     6361 caaataagga aagtaccatt aaaaaagact ctaagaataa ccaaagccaa gttaaagaag
     6421 aaaaaagaga acctgtgaaa aaagatataa ctataagttt cgctggtgat gtaactatgg
     6481 ggaactataa aggttcatca tattatggca gttttgacca tgaatttaaa agacaaggag
     6541 aagattacaa ttattttctt aaaaatgtaa aacctgtatt tgaaaaagat gacttaacag
     6601 tggttaattt agaaggtcca cttactacag cgtcaaatgc aaaagaaaaa aaatttgcat
     6661 ttaaaggaaa gcctggatat gtaaatattt taaaatctgg aagtgtagag gcagtaagta
     6721 ttgcaaataa tcattcagaa gattattttg aagaaggaat gaaagataca cagtttatat
     6781 tggacgagaa taaaataaat tattttggat tagggaaaga tgctgttgta gatgtcaaag
     6841 gaattaaagt gggtctatta gggtataatg gattatcaac tgaatataat gaaaaaaatt
     6901 taaaacaaat ggaagatgat attaagtcct taaaaaagaa atcggatgtt gttgtagtgt
     6961 actttcattg ggggatagaa ttagattatt atccagataa aaaacaaaaa gattttgctc
     7021 attatgctat agacaaggga gcagatttag ttatggggtc tcacccacat gtgattcaag
     7081 gaatagaaaa gtataaaaat aagtatatag cttatagtct agggaatttt tgctttggag
     7141 gaaataagaa tccttctgat acagatagtt atatatatca acaaaccttt actttttcag
     7201 ataataaatt aacttcaatt aaagagccaa atataatacc aacttcaata acatctagta
     7261 actccagaaa taattatcaa ccaaaaatat tggatggaag tgagaaggat agagttttaa
     7321 ataaacttga aaaaataagc aaagatttaa ataaataaaa tgtaaaaaaa aataaatata
     7381 tatatttatt ttaataattt atattttgtt tagggtatgg taaatcgtgg tattaaataa
     7441 gtagatttta atagttaaat tatagtcaat tataataaag ctatttaatc tactttttta
     7501 atataaaaat ataaggggga atttgtaatg gcttgtaaca cttgtaaaat gtgcgaaagt
     7561 gcagacaaga aattagaaag ttttgtagct tcaaaggatg ttgaaacagc atttcataga
     7621 acagaagatc agaaagttaa gtgtggtttt ggacttcagg gggtttgttg tagactctgt
     7681 tcaaatggac catgtagagt aactcctaaa tcaccaagag gtatatgtgg agcagatgca
     7741 gacactatag tagcaagaaa ctttttaaga gcagtagcat caggagcagc ttgttattta
     7801 catgtggtag aaaatacagc taagaattta aaaaatgttg gaataactaa aggtgttgtt
     7861 aaaggagaaa aaacattaaa tcaattagca gagatgtttg gaatagaaag tgatgaaaag
     7921 tatgataaat gtataaaggt tgcagacaaa gttatcaacg atttgtataa atcaagagat
     7981 gataaaatgg aattagtaga aaaaatagct tatgcaccta gagtgaaaaa gtggaaagag
     8041 ttaggtataa tgcctggtgg tgcaaagtca gaagtttttg atgcaatagt aaaatcatca
     8101 acaaatttaa acagtgaccc agtagacatg ctagttaatt gtttgaactt aggtatatca
     8161 acaggattat atggtcttac actaactaat cttttaaatg atgttatgtt gggagaacca
     8221 gttataagaa tggctcctgt aggatttaat gtaatagacc cagactacat aaatataatg
     8281 ataacaggtc atcaacattc aacatttgca aatttccaag atagattaaa agatgaagat
     8341 gttataaaat tagctcaatc tgttggcgca aaaggattta agttagtagg atgtacatgt
     8401 gttggtcaag atttacaatt aagaggagaa cattatcaag aagtatttgc tggacatgca
     8461 ggtaacaatt ttacaagtga ggctgtttta tcaactggag caatagatat agttctttca
     8521 gaatttaact gtactatacc agggcttgaa ccaatagcag ataaatataa agtaaaaatg
     8581 gtttgtttag atgatgttgc taaaaaagcg aatgcagaat gcataggttt agacagaagt
     8641 aaattagatg aacttagtaa cactttaata gaaaaagcat tggagtctta taaggagaga
     8701 agaggaagta ttgaaataga tataccaaaa gaccatggat ttgaacaatc tttgactggt
     8761 gtaagtgaaa agaacttgaa agcgttttta ggagattcat ggaaacctct tattaattta
     8821 attgcagaag gaaaaattaa aggtgttgca gctgttgtag gatgttcaaa tatgacagca
     8881 ggaggacatg atgtaaatac tgttgaactt actaaagaat tgattaaaaa agatataata
     8941 gtactatctg caggatgttc tactggagga ttagaaaatg ttggtcttat gtcacctgga
     9001 gcagaagaat tggcaggaga aaacttaaaa gaagtatgta agactttagg aataccacca
     9061 gtattaaact ttggaccatg tctagctata ggaagactag aaatagtagc aacagaatta
     9121 gcagctgaac ttggaattga cctaccacaa ttaccattag tgctttcagc tccacaatgg
     9181 ttggaagaac aagcattagc tgatggagct tttggtcttg cccttggctt acctcttcat
     9241 ttagctttac ctccatttat aacaggagga aaattggtaa cagaagtatt aacagaaaaa
     9301 ttaaaagact taacaggagg acatgttata gtgaatccag accctaaatc ttcagctaat
     9361 caattagaag aaataataat agatagaaga aaaaatttag ggttaaagga tgtggaatgc
     9421 aatgcataga atttttataa ataaagattt atgtacagga tgtaagagct gtgtactagc
     9481 atgtatgcta aaacacaata aagattatga catgtacact cttgatttag aaaatataga
     9541 taatgatagt agagggcata tagaattaga tagtagacat aataatccag ttccaattct
     9601 ttgtagacac tgtgatgaac ctgaatgtgt attagcatgt atgagtggtg caatgcataa
     9661 agatagtgaa agtggaatcg tttcatatga tgaagagaaa tgtggttcat gttatatgtg
     9721 tgtaatgtct tgtccatatg gattattaaa gccagatgat agaagcaagc agaacatact
     9781 taaatgtgat ttatgtaaag atgaggagta tcctagatgt gtagctaatt gcccaagtgg
     9841 agcaattgaa ttacagaagg aggaaaatga tgaattatgt agtgttaggg gctagtgcag
     9901 ctggtataaa tgcagtaaaa acattaagag aattagataa agattcaaat atagtagtaa
     9961 tttctaaaga tgaaaacgta tattcaagat gtatgttgca tcacgttata tctgaacaca
    10021 gaactttaaa acaaataaat tttgttgatg aagattttat ggagcaaaat aatgtaaaat
    10081 ggattgcagg taagactgtt aaaggtatag acataaataa aaaggtagtt cagacagaag
    10141 acattactgt aaattatgat aaacttttaa tagctacagg tgcatcttct gctattcctc
    10201 ctataaaaaa tttaagagat ggaaattttg tatattctgt gagaaacata gatgatattt
    10261 ataaaataaa agaaaaagca caaaattcta aaaatgtagt tataataggt gctggtttag
    10321 ttggtataga tgcattagtg ggattattta aatatgagca gttaaatatt tctgtagctt
    10381 ttatggaaaa gtacatttta gatagacaat tggatgaata tacagcatca gtgtatgaaa
    10441 ataagttcaa agaaaaaggt gtaaaatttt acccaagtgc atcaatacaa gaaattgtat
    10501 tggataacag taaaaatgtt acaggtgttg cattttcaaa tggtgaagtt ttagatgcag
    10561 acatggtaat tgtagctaca ggggttaaac caaatgctga ctttttagat ggaacaggaa
    10621 tagaatatga tagaggtata ataatagatg atatgtgcca aacaacacaa aaagacatat
    10681 atgcagcagg agatgttgta ggtaaaaatg ctatttggcc tctggcagta aagcaaggaa
    10741 ttgtagctgc ctataatatg gttggaaaag ataagaagat agaagatgaa tttgctttta
    10801 aaaatagtat gaatttcatg gatataccaa ctatttctat aggaatgaat acacctgtag
    10861 atgatagcta taaagtatta accagacatg gaattaatga ttataaaaag tttgttttta
    10921 aagataatgt aatctatgga gcagttatac aaggagatat ttcatatgta ggtgtattaa
    10981 cttatttaat aaaaaataaa gttgaaatat atgatttaga aaataggata tttgatatat
    11041 gttatgcaga cttttttaat ataaaagaaa atggagaatt ttgttatagt gtatagtata
    11101 tacgtggagt aaggtttata aatacagtaa atcaataaat agagttattc taaaataaga
    11161 aaaatgacaa atcttattta gaaatagctc tattttttgt aattaaattt aaattatatt
    11221 caatttgtaa aatattattt tgaacaatta tacatatact ctctagttag aggtaaatca
    11281 ttttttatac ctttagtgaa aagtatttga tgtatatcta taataccagt gttgaaagca
    11341 gcagcacaag aacaaaggta taaatcccac attcttataa acttatcatc aaacatttct
    11401 ttaatctttg gaagattctc agagaaattt ttttgccagt atagtaaggt ttttacataa
    11461 tgtaatctta aactttctac gtctatagta tgatatttgt aatcactaca aatatgtatc
    11521 atttctctta ggctaggaat aactcctcct gggaaaatat atttttttat ccaagggtct
    11581 ccataagatt cctgcaatcc acttatatag tgaagtaaaa acaatccacc atcttttaaa
    11641 acagcattta cgtttttaat gaaaagttca tagttttctc taccaacatg ttcaagcatc
    11701 ccaacgctta caactctatc aaacataagg tctgattttt ttaattctct atagtccata
    11761 agtcttacct caagatagtc ttgaaggttc tcttctttaa ttcttgcgtt aaattcctta
    11821 aattgctctt cgctaagtgt aattccaacg ccgtgaattt tatatttttt tgcagcttct
    11881 attagtaaaa atccccaacc acaaccaata tcgagaaggc tcataccttc ctctaaattt
    11941 agtttagtta atatgtggtg aactttattg acttgagcat cataaagagt atcattatca
    12001 tttctaaagt atgcacaaga ataactaagt gtcttatcta accataagct ataaaaatcg
    12061 ttgccaatgt catagtgggc atgtatttct ttcttttgat gtctcattga atttgatgag
    12121 tgcaatagat gttttacagc tgaattatca atattgaatt tgtctatttg ttttaaaagt
    12181 agatttaaag ttaagaataa atctccctca acttcaatgt ctctatttat gtaggcttct
    12241 ccaagtgcta aagatgtact ttttaatagt tcagacttgc taatatcatt atttactata
    12301 actttgaact gaggggagcc ctctccaatt agaaattctt cctcatcttt aagcctaact
    12361 agaaatggtg tttcaccaaa tttctctaaa tagtctttta aaaaattttt tgtaatattc
    12421 atattaccag ctccttctaa tgtaaatcca gtaagtttcc ctgcttaaaa tatattatag
    12481 tccttttttt aaaaaagtaa aattttttca ataaattttt tatttaaagg aaatttattt
    12541 tttgtaaaga aatttcatgt tgtagtatat tttttgctta taaattttgg tactggatat
    12601 aatattaaac tgttaatgat ataataaatt taatctataa agtaaataaa attttgtaat
    12661 aaatagttaa agctatttta taaggagata tatgatggaa gtatatgctt tggttgggtc
    12721 aagtggcaca gggaagagct ttaaggcttt agagttcgca tatgaaaatg atatagaata
    12781 tataattgat gatgggattt taatatacaa aaacaaggtt ttagctggga tatcggcaaa
    12841 acaagcaaat actactatag aagcagttaa aagggctatt ttttataatc tggagcatag
    12901 acaagaagtt agagaagcaa ttaaaaaaga aaatataaga aaaattctta ttattgggac
    12961 atcaaaaaaa atggttaatc aaattgtaga gagattgtca attggaaggg tttgcaagtt
    13021 tataaatata caagatataa gtactaaata tgaaatagaa atagcaaaac aagctagaaa
    13081 agaaggaaat catgtaattc ctgttccagc agttgagata aaaagtatgg caagtggtct
    13141 tagtataaat tctttgaaga gactttttag aaaaggaaat aataggaata tggtagtttt
    13201 agaaaaaaca attataagac caacttttag ttatatagga aagttttata ttagtgctga
    13261 tgtaattaaa cagattattg aatatgaagt atacaacttt ggaagcattg atagaataaa
    13321 taaaatcaat atagagaatt gcaataattt tatgaatatt tttgtaagta taaatatcaa
    13381 tgatttagaa actataaaaa aaattgaaga catacaacag acaataaaaa agtctattga
    13441 gaaaatgact ttagttaatg tacaaaaaat agatattaac attcataaaa ttaaaaaaat
    13501 aaacttcctc caatgttaaa aaataaactg agaaaatgat atactaattt gcaataaagt
    13561 gtgctataat taaactgtaa atttaggaaa tggttttttc taaatggagt atacataatt
    13621 ataacgtatt tataaggggg ctttcaaatg tcaggaaaag atgtaaatgt cttcgagatg
    13681 gcgcaatctc aagtaaaaaa tgcatgtgat aaattaggta tggaaccagc agtttatgaa
    13741 ttattaaaag aacctatgag agttatagag gtttcaattc cagtaaaaat ggatgatggt
    13801 tctataaaaa cttttaaagg atttagatca caacataatg atgcagtagg gccaacaaaa
    13861 ggtggaataa gattccatca aaatgtttca agagacgaag taaaagcttt atctatatgg
    13921 atgactttca agtgttctgt aacaggtata ccatatggtg gaggtaaagg tggaataata
    13981 gtagatccat caactttatc tcaaggtgaa ttagaaagat taagtagagg atacatagat
    14041 ggaatatata aattaatagg tgagaaagtt gacgttcctg caccagacgt aaatactaat
    14101 ggtcaaataa tgtcttggat ggttgatgag tacaataaat taactggaca aagttctata
    14161 ggtgttataa ctggtaagcc agttgaattt ggtggttctt taggaagaac agctgctact
    14221 ggatttggtg ttgctgttac tgcaagagaa gctgctgcta aattaggaat agatatgaaa
    14281 aaagctaaaa tagctgttca aggtatagga aacgttggtt cttatacagt tcttaactgt
    14341 gaaaaacttg gtggtactgt tgtagctatg gctgaatggt gtaaatcaga aggttcttat
    14401 gctatataca atgaaaatgg tttagatggt caagctatgt tagattatat gaaagaacat
    14461 ggtaacttat taaacttccc aggagctaag agaatatctt tagaagagtt ctgggcttca
    14521 gatgttgata tagtaatacc agctgcatta gaaaactcta taactaaaga agttgctgaa
    14581 tctataaaag ctaaattagt ttgtgaggct gctaatggac caactactcc agaggctgat
    14641 gaagtatttg ctgaaagagg aatagttctt actccagata tattaactaa tgctggtgga
    14701 gttacagttt cttacttcga gtgggtacaa aacttatatg gatactactg gtcagaagaa
    14761 gaagtagagc aaaaagaaga aatagctatg gttaaagcat ttgaatctat ttggaaaatc
    14821 aaagaagagt acaatgttac aatgagagaa gctgcttaca tgcattcaat taagaaagtt
    14881 gctgaagcta tgaaattaag aggatggtac taagatttac tcttaataaa taataggata
    14941 taattattta agatataaag gggctgtaca tagtacagcc cttaaattat gtggcttaca
    15001 atgaagtaag ctatttttgt tttaacttat aaataacttg ataaattatg taatttagaa
    15061 tgtatttttt accaccacat gattacaaat tagaggagtt agattgaatt tttagacatg
    15121 aacttatgca ttttaaaagc aaagacctta atttaaagta tctagtatta tttttaaaaa
    15181 tagtatattg gtttaatcca tttatatgta taatggaaaa atatatggat ttggattgtg
    15241 aactttattg tgatgagaga gttttaaaaa attgtactgt acaacaaaag caagaatatg
    15301 ctctaactat tgtaaattca atgaaaagaa gtacaaattt ttcaaataaa tttgtagctg
    15361 gattaaataa acaatctgat attagaaaaa gagtaataaa catgttcagt gaaaaacata
    15421 aaaatggtat cttaagggct ttgattataa gcttgttttc ttctattaca ttttttaaat
    15481 tcgattcaac tagtattatt gaagagaatg atttcttaga gaggtcaaga tatacgtttg
    15541 aaggaaggca agaagacatg tttgtaacaa tagattttat atatgcagat gtacctataa
    15601 aacttagaaa agagcatgaa gaaaactgta gagtcaatgg aatagtacca aaagattcag
    15661 attcaataga aattattcct agattttata aagattgtat ggatttaaaa aaataagtta
    15721 gacttaatat tgatgttgat attatgaatt ttttatattt aagcaattga aaattttatt
    15781 atagattcaa ttttatatgt gtttaagtat gaaatttata gatagttgaa ataattaaat
    15841 atgattgatg cataggaatc attactaagc tataattaat tataggggag tttaaataaa
    15901 ttatgagtta cacaatcata aaatgataaa aatagttatc tgttacaaaa aatgtatctt
    15961 tttaattaat aaagataata tgaaaactta aaaggcaaat aaacttaaat ataaaatact
    16021 cttaaataag ataggagaaa aatgtatgtc taactttatt ctaaatggga tttttagaac
    16081 agttatagta agtagtctaa gcattcttct gatattgatt ttcaagaaaa atgtatttaa
    16141 aagatttagc aaaaaattta attattatat atggatgata gttgttatta aattattttt
    16201 accatttaca tattatactt ttacaataaa tatcttgaga agtaaaaaac acattaatat
    16261 taataacata aatttagagg gatttaataa tgtttcaact ataagtaata atattatatt
    16321 gtacacttgg attataactg tagtagtgta tttaatttac actatattta aatatataaa
    16381 acttaaaaat ttaataaatg acttatctta tgatgttgat gatagagaga ttgtaaatct
    16441 ttataaaaat atattagaag aatttaatat aacaaaagat ataaaactca aatactctta
    16501 tgaagttgaa acaccagcat tttttaattc atgtgtactt ttgccaccac atgagtataa
    16561 attgaaagag ttagactgga tttttagaca tgagcttatg cattttaaga gtagggacct
    16621 ttatttaaaa tatgtaatcc tatttttaaa aacagtatat tggtttaatc catttatata
    16681 tataatggat aaacatatag atttagattg tgaactttat tgtgatgaaa gagttttaaa
    16741 aaatcgaaat tcagaggaaa aaaaggatta tgctttaaca attctaaatg caatgagaaa
    16801 aggttcaaat acttcaaata aatttatagc tggacttcat aagcaatctg atattaaaaa
    16861 gagagttatg aatatgttta atgaaaagta caagaatggt atattaatgg cattaacttt
    16921 atgtttatta tcttctatta cttttttgaa ggttaattca actagtattg ttaatcttaa
    16981 tgtattacct aaaggatttg aagacttacg aactgttagc agtatacgtt tttcagttat
    17041 aggatttaca tatgcagatg ctcctaaaaa tatgagggaa gaatataaaa taaattgtga
    17101 atcattaggt ttgataccta aagactctga taaaatagaa gttggtatag aatattatga
    17161 cagtctattg aatgattaaa taaatattat tttaacagat attaattata aagttttgaa
    17221 tagtaatttg ttgagaaata aatatatcct tataaaaata gatacttata taccaagaat
    17281 aaaaaatagt caagatagta cacaaggata tacttataaa gaacttaaat aagtgaaata
    17341 aattattaat tataaggttt ataattttaa aaaattagat aaattcaggt gtaaaatact
    17401 ataaaacaca attcggaaaa gtgtatgttg aacttcattt aaaatggaat ttcaaagttg
    17461 ctatagtaag tagtttaagc attcttctta tattaatctt tgagaataat gtatttaaaa
    17521 aagttagtaa aaaatttaac tattgtatat taatgatagt tgctattgat atattatcta
    17581 aaaaaattaa gtatgaaaag attactgcta ggacaatctt ttcatactta atttttaata
    17641 tttatattat ccaaaatttt catcttttta acttagaatt tgagaatcac tattaggaaa
    17701 taggctttga tttatgtagg gttaaaaatt tcacagaata tctaacttta tactaaatcc
    17761 atcaaaaaat atacgccata caaaagcgca taaaaaaaca cataaaacaa tatttatgcg
    17821 cactttaacc tcgaatggta caaaattctt tacttttaca gacccatatt atataattaa
    17881 gtaaataatg tatatttagg tgacatattg gataagctaa tttatttatt ttattaatat
    17941 aataatacta taaaagttat ataaagtggt ttacaaaaaa gttacagata gagcttttat
    18001 taatgtattg tgtattagta ttttatcctt aggtacactt cacctacaaa atagaatcat
    18061 aagataatta tgggaaaatt ttaggaggtt tatcgtgaag aaaaagatat taataccagt
    18121 atttgcatca gttatggcat tatcagtaag ttcaattgta aatgcagatg aagtaaatga
    18181 ttcatctcaa aataaggatg ataaaacaaa tactgaatta aatttaggtg aatataaaga
    18241 agtaaaatat aaagttgcaa aaataaaaga tggagtagct ataaagataa gagaagaagg
    18301 tcaagttcaa aacatagctt attctggaga tgaatttacg gttttaggaa ctcaaggaga
    18361 atgggttaag gttaaagttg aagatggcga aggttggtta gctactagat atgtagatat
    18421 atctgaaggt gtaggatata ctaacgctga taaagtaaac ttaagaaaag ataagagtga
    18481 aagttcagaa gtaattgaag agctggaaaa aggaagttct ttattggttt tggaagataa
    18541 tggagattgg ttaaaagtta aagatgggga aacagaagga tatgtaaaat catcttatat
    18601 atctgataaa gctcctgtaa tagaagagcc acaagtaaac ccaagtattg accaaaatgt
    18661 agattctaca aataataatc aacaaaacaa tgcaaatact aatcaacaaa ataataattc
    18721 aaatgtgcca acagctaact ctaatgcagt tcaagctgta cttaacttgg catattctaa
    18781 gcaaggttgt ccatatgttt ggggagcaga aggaccaaac acttttgact gttctggatt
    18841 tacacaatat gtgtatagaa atgcagtagg aaagaatata ccaagaacat ctaaagcaca
    18901 atctaaatat ggacaaactg taagtaaagc aaatttacaa ccaggagatt tagtattctt
    18961 cacaactaat ggttctggtt ctgttagtca tgttggaata tatgtaggtg ggggtaatat
    19021 gatacactca ccatcaacag gaaaaacagt tagtgtaact agtataaatt catcatacta
    19081 tacagcaaga tttgttacag caaaaagaat attataatcg gtttgtaata tgacaaattg
    19141 ataaatacat aaatatatat aaacgctcta tattatattt aatttaatca tggaaacaaa
    19201 ataatgatta tattttaaat gtactataga gcgtttttgt atcactttaa atctaatcaa
    19261 gattcaagtt aatatgactg ttaaatagga gccttttcat atgatgatta tttgtgcaat
    19321 cccttgacag gatagggtaa aggtggtaag ctactaacag tacaaaaata cttttaattt
    19381 tagttcagag aggctaaaaa aggaggacga aagatgttaa aatcaagaaa tttaatccaa
    19441 ccagaagact tttcaataga agaaatagat gaaatattag aattagctca aaagataatt
    19501 gacaatccgt caaaatattc tcgtatatgt gaaggaaaac tattagctac attattttat
    19561 gaaccatcaa caagaacaag attaagcttt gaatctgcta tgaacagatt aggaggacgt
    19621 gtagtaggct tttcagagcc aaattcatcc tctgcatcaa aaggtgaaac tctaggagat
    19681 acaatgagga tagtatctgg ttatgttgat ataatagcta tgagacatcc tcaatcaggt
    19741 gcagcttcag aagcagctag atatacggaa gtgccattta taaatgcagg agatggcaaa
    19801 aaccagcatc caactcaaac acttacagac ttacttacta taaaatcatt aaaaggaact
    19861 ttagaaagcc atacaatagg attatgtggt gatttaaaat atggaagaac tgtgcattct
    19921 ttggttaagg ctatggcaag atataaaaat acaaagtttg tatttatagc acctgaagag
    19981 ttaaaaatgc cagattatat aaaagaagct ataaagggac atgcatatta tgaaactaat
    20041 aatttggatg atgttatagg aagcttggat gttttatata tgactagagt tcaacaagaa
    20101 agatttgaag ataaatcaga gtatgaaaga cttaaaaact attatatttt aaataaagct
    20161 aaattagaaa aggcgtctaa agatatgtta gtaatgcatc cattaccaag agtaaatgag
    20221 atagacatag atgtagatag tgatgataga gctgtatatt ttaaacaagc taaatatgga
    20281 atgtatgtta gaatggcttt aataataaaa cttttaggta taaatgaaga ctaatagtta
    20341 tcatataata gtaaaacaaa ggagattttg ggatgtataa aattctggaa aatatatata
    20401 taggcgaaga tatgtataga atgaaagtaa aaggtaattt tgaaggtaaa atgggacaat
    20461 tctatatgtt aagagcatgg gatacttatc cagtactttc aagacctata agtatacatg
    20521 atatagatga agaagggata acttttcttt ataaagtagt aggagaagga actcaaattt
    20581 tgagtaacct aaaggtaaat gatactataa aattggaagg cccttatggt aatggatatg
    20641 ctaaggttga tggaaaggta gctctagtag gaggaggaat cggtgtagct ccactatatt
    20701 tggttgctaa aaatataaaa aattgtgatg catatcttgg ctttagagaa gatgttatat
    20761 tagaagatga atataaacaa gtatgtaata aagtatatac aacagtagga aatacttttg
    20821 taacagatat aattgatgtt gagaaatatg attatatatt aacatgtggt ccaactccaa
    20881 tgatggaaaa attagttaag atggtagaag gtactaaaac gagaattatg gtatctcttg
    20941 aaaaccatat ggcttgtgga gtaggtgcct gtttagtatg tacgtgtaaa actaatggag
    21001 gaaataaaaa gacttgtaaa gatggtccag tgttctgggg agaggacgtg atttttaatg
    21061 ggtaatttaa gtgtgaaatt tggaagtgta gaatttaaaa atccagtaat aatggcgtct
    21121 ggaacttttg gatttggaaa agaatataat gagatttatg atatacaaaa acttggtggt
    21181 ataagtagca agggtcttac tttaaataaa aaacctggta acaatggtat gagagttcat
    21241 gagacaccat ccggtatgat gaatagtgtt gggcttgaaa accctggagt tcaaggattt
    21301 atagactatg aattaccttt ttttagtaaa ctagacttag tcagaatagc taatgttgga
    21361 ggtgggacat tagaagacta cttattaggg gttcaaatgt taaatgacaa gcctatagac
    21421 attattgaat taaatatatc ttgcccaaat gtcaaagctg gtggaatggc ttttggtata
    21481 aaaaatgaag tggcaagaga agttgttcgt gaggtaagaa acataacgaa actgccttta
    21541 gtaataaaat tatcacccaa tgcagaagat attgtaggaa tggcaaaagt ttgtgaagaa
    21601 gaaggtgctg atggagtttc attagttaat acttttaaag caatggcaat tgatataaaa
    21661 aatagaagac cagtatttga aaatgtatat gctgggttat ctggaccagc tataaaacct
    21721 atagctttga gaatggtaca tgaagtgtgt aaaaatgtaa atatacctgt aatgggaatg
    21781 ggcggaataa ctaaagccac agatgctata gagtttataa tggcaggagc tacttgtatc
    21841 caagttggaa ctgcaaattt tatgaatcct agaataggta ttgaaataat agatggaatt
    21901 aatgaattta tggatagaga aggaataaaa agtttagacg aaataagagg aattatataa
    21961 ggtagaataa atagtatttt aaagataaaa aatatagatt aaataaacaa taattttata
    22021 acattaacat ggaggaaaaa taatatgagt aaagtagatg tagtggatat attaaagaaa
    22081 agtgatgcat tgttggaggg acatttttta ttatcttcag gaaaacacag taatagatac
    22141 gtacaatgtg caaaagtatt aagatttcca gaatatgctg ctgaggtatt aagtacagtt
    22201 gttgaacaaa taaaagactt agatatagac ttagtagtag gaccagctat gggtggagta
    22261 atagtttctt atgagttagg aagacaatta ggaaaagaag ctgtatttac tgagagaaaa
    22321 gacaatacaa tggagttaag aagaggattt gaagttaaaa aaggagcaaa gataataatt
    22381 gctgaagacg ttgtaactac tggtaaatca actatggaga caaaaagagt attagaagcc
    22441 ttaggtggag aagttgtagg tgttgcatgt atagcagata gaactaatca tgatataggt
    22501 atgcctatat atagtgctat aaaacttgat attcaagttt atgaatctga tgagtgtcct
    22561 ttatgtaagg aaggaaaatt accagttgtt aagcctggaa gtagggagtt caaggaatta
    22621 gggatgtaat aaaaacttaa ttatttatag tgttacttaa aaaatgtaaa ttttttagag
    22681 taaaaatttg taatataaca tataacttaa aaataaaaag cagttagcct tataaatgaa
    22741 ggttagctgt tttttttgtt aaaaatatat ttacaaaata agagatagta gcagtacaat
    22801 ataagtataa ggtaaaagta ttatatatta gggaggggta agtatgaaaa atcaattaaa
    22861 tgaaaatcat aaaaaggttg tattttttgg agtaggtgct gtaggagcta cttttgcaga
    22921 acaatttttt aactctaaat atgattttaa aattctttgt gataatgaaa gaaagaaaag
    22981 atatttagaa gaaggattta taataaatgg aaaaaggtat gattttgatt atgtaactaa
    23041 agatgagtat aaacaagagg ctgattttat aattataggt ctaaaatata ataatttaaa
    23101 agaaaatata aaagaattag atggattagt tggaaaaaat acagttataa tgtctctgct
    23161 aaatggagtt gatagcgaag agataatagg agaaagattt ggaattgaaa aaatggtata
    23221 ttcatatgtt accaatatag atgcaaagaa aatcaataat aatattatac acactactaa
    23281 tgggataatt gtatttggta acaaagataa tagtgaagat agaaaaacta atataataac
    23341 cgaagtcttt gatgatgtaa atatagaata tactttatca aaagatattc aacgagatat
    23401 gtggtggaag tacatggtta atattggtgt aaatcaaact tcagctatac ttggtgcacc
    23461 ttatggagtt tttcagagtt ctgagcattt aagagaatta gcaaaatctg caatgaggga
    23521 agttgttgct atagcacaag caaaagacat atctcttaca gaagatgatg tggaacattc
    23581 attacataga atactagaac attcaaaaga aggaagaaca tcaatgcttc aagatgtgga
    23641 agctcataga cttacagaag tagatatgtt ttctaagaat atctgcaaac ttggaaaaaa
    23701 atataacata cctactccta taaatcagac tttcttttat atgataaaag taattgaaag
    23761 tagattttaa agggtctttg taagaaatat tgataattat tgattttaaa ttttattaat
    23821 gttatatact atttaagtta ctaaatattt ttaactcttc aatgaaaata gttgctgctt
    23881 ttgataaagt tatatctttt ggaaatatat atgagaaatg tctgttgtag ctgcttccaa
    23941 gctctatgac agacagctca ttgtttagaa caggtaagct tgtcactaga ttagagacta
    24001 ttgtaattcc taaattattt ctaacagatt ctttgacagc ataattactt ccaaaaacca
    24061 tcatactttt tggaataatt tctttaacac ttaaaaacat atctaaataa tctctggttc
    24121 cagaaccatc ctccctgact atccattttt ggttttgaag tttatcaaaa ctaaaatctt
    24181 ttaataaatg actcttgtat ggaagtgcta aaaccatctt atcttcaaaa aaatattctt
    24241 gaataaatga aggggaagag caagtgcctt caataagacc aatgtctaaa atataatcct
    24301 taacatgaga agatacgatt gatgtatttt ttataaaaac ttctacatct atgtcaggat
    24361 actttttaga aaataatgct agaaaatttg gtaaaatata ctctccaata gttaaacttg
    24421 caccaatctt taagtgacca gttatagcat ctgaaacttg gcttacatca tgataagtaa
    24481 tatttaaaag atttaatatt tctttagctc ttttgtacaa gatttgtcca ctttctgtta
    24541 taaaaatggt tttttgttta atagacctgt ttataagcac aactttaaaa taattttcta
    24601 gattttttat gtgtttactt acacttggtt gtgatagatt taaatattcc ccagctttag
    24661 taaagttttt gtattcaaca acagctacaa atgtttttaa ttcttcaaac aattttttgt
    24721 ctcctttacc taatgaaatt ttacattttt tatatacttt ctacagataa ttatactata
    24781 tataataacc attaaaaaca ttaatcatta cgatattata tatttatttt acttatggtt
    24841 attacaatta tatactattg ctatgaagaa aactatatta attataaggt aggtatatta
    24901 tgttaaagaa aaaaagcagt ttgacattaa aaactataag ggaaatatta ccaggactgt
    24961 ttgtatcagt attagtaggt tatattagta tatttatttc tattttaatt cctaaggtag
    25021 gggcagcatc aatttctata tttttaggta tgtttgtagg aaacctattt ttaaatcaaa
    25081 aggtatttca aaaaggttat aaattttcag agacagattt gttatcatat tcaatagtac
    25141 tattaggagc aacactcagt gtatctacct taatagactt aaaagtttca ggaattttat
    25201 ttataatatt gcaaatgaca ataacaataa tagctgcact ttatataggt aaaaaactag
    25261 gatttgaaga aaactttaga tttctaatgg caagtggaaa tgcagtatgt ggttcttctg
    25321 caataggagc aacagttcca gtggttaatg cgacagataa agaaaaggga attgcagtaa
    25381 ctattgtaaa tgttactggt atatttctta tgtttttact tccaataata tctcaatttt
    25441 tatatagcca tgaacttgta aaaacttcag caatgatagg tggaactttg caatcaattg
    25501 gtcaggttgt agcaagtgga gcaattgttg gagaaaatgt aaaagattta gctactattt
    25561 ttaagatagt aagagtaata tttctagttg ttgttgtatt agtatttgga catatgaaaa
    25621 atcaatctaa tagggaaatt cttgaagaag aaaaggatga gataaaaaag aaaaaagtga
    25681 ctataccatg gtatgtaatt ggattcttta taacttgtgc acttttttca atgaacatta
    25741 taccacaaga agtatctgtg ttgtgcaaag aaataagtaa caaactagaa ataatagctt
    25801 tagcagcaat tggattaaag gttaatgtaa aagatttagt gaaacaaggg aaagaagttt
    25861 ctttatatgg tttatttgta ggtatagtac aagttgtatc agctgtagtt ttaataaaaa
    25921 tatttatata gcataaaaat agtatttgta atattaattt gattaa
//
LOCUS       DAMKMI010000041        25962 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMKMI010000041 DAMKMI010000000
VERSION     DAMKMI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.042"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(98..1105)
                     /locus_tag="PC429_001623"
     CDS             complement(98..1105)
                     /locus_tag="PC429_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF3847135.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            1333..2217
                     /locus_tag="PC429_001624"
     CDS             1333..2217
                     /locus_tag="PC429_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3847136.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            2419..3969
                     /locus_tag="PC429_001625"
     CDS             2419..3969
                     /locus_tag="PC429_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3847137.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETEDLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            3998..4645
                     /locus_tag="PC429_001626"
     CDS             3998..4645
                     /locus_tag="PC429_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF3847138.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSILLFVMYKLCKSLSIKIFQC"
     gene            complement(4787..5047)
                     /locus_tag="PC429_001627"
     CDS             complement(4787..5047)
                     /locus_tag="PC429_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF3847139.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMSKFQDLVKITSKK"
     gene            5264..6547
                     /gene="hflX"
                     /locus_tag="PC429_001628"
     CDS             5264..6547
                     /gene="hflX"
                     /locus_tag="PC429_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF3847140.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            6555..7232
                     /locus_tag="PC429_001629"
     CDS             6555..7232
                     /locus_tag="PC429_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF3847141.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            7318..7761
                     /locus_tag="PC429_001630"
     CDS             7318..7761
                     /locus_tag="PC429_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF3847142.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            8066..8710
                     /locus_tag="PC429_001631"
     CDS             8066..8710
                     /locus_tag="PC429_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF3847143.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(8796..9173)
                     /locus_tag="PC429_001632"
     CDS             complement(8796..9173)
                     /locus_tag="PC429_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF3847144.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(9173..9559)
                     /locus_tag="PC429_001633"
     CDS             complement(9173..9559)
                     /locus_tag="PC429_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF3847145.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            9738..10493
                     /gene="nadE"
                     /locus_tag="PC429_001634"
     CDS             9738..10493
                     /gene="nadE"
                     /locus_tag="PC429_001634"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF3847146.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            10576..11295
                     /locus_tag="PC429_001635"
     CDS             10576..11295
                     /locus_tag="PC429_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3847147.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(11462..12466)
                     /locus_tag="PC429_001636"
     CDS             complement(11462..12466)
                     /locus_tag="PC429_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF3847148.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQEFILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            12732..13628
                     /locus_tag="PC429_001637"
     CDS             12732..13628
                     /locus_tag="PC429_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF3847149.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            13766..15028
                     /locus_tag="PC429_001638"
     CDS             13766..15028
                     /locus_tag="PC429_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847150.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVDYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            15104..16297
                     /locus_tag="PC429_001639"
     CDS             15104..16297
                     /locus_tag="PC429_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF3847151.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSVKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            16926..17720
                     /locus_tag="PC429_001640"
     CDS             16926..17720
                     /locus_tag="PC429_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF3847152.1"
                     /translation="MESVMENLNNLKVELKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            17815..19155
                     /locus_tag="PC429_001641"
     CDS             17815..19155
                     /locus_tag="PC429_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF3847153.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            19292..20524
                     /locus_tag="PC429_001642"
     CDS             19292..20524
                     /locus_tag="PC429_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF3847154.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            20564..21706
                     /locus_tag="PC429_001643"
     CDS             20564..21706
                     /locus_tag="PC429_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF3847155.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            21717..22511
                     /locus_tag="PC429_001644"
     CDS             21717..22511
                     /locus_tag="PC429_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF3847156.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            22522..23544
                     /locus_tag="PC429_001645"
     CDS             22522..23544
                     /locus_tag="PC429_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF3847157.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            23643..25019
                     /locus_tag="PC429_001646"
     CDS             23643..25019
                     /locus_tag="PC429_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF3847158.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(25351..25695)
                     /locus_tag="PC429_001647"
     CDS             complement(25351..25695)
                     /locus_tag="PC429_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847159.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT         9805 a         3041 c         4843 g         8273 t
ORIGIN      
        1 ctaatatatt tggtctgtag gttaaataaa agtaccttta gaccaaatat attattaata
       61 acttaataac atcttaaaac ttatttattt atattattta caacatccat ttaataaata
      121 caccataacc tacagctggg tcgttttcaa ttgcatgtga cactgctccg attattttat
      181 tatcttgaac tattggagta ccactcatac cttgtacaat accaccagtt tgagctaaaa
      241 gctttttatc tgtaatttta attttaaaac ttttactttc tggatgtttt tggttttcta
      301 tatctataat ttcaatatta tattttttac acttattaaa gttatcttgt aaaattattt
      361 gtgcttttcc taatttaact tcatctaaag atgctacttt atattttttt aacttggaaa
      421 tatcaaagtt attaatttga cctcttattc caaaatcagt attttctgta aagctaccaa
      481 taatattttc tcgttttgca tttatacatc caacactacc tttataagat ttttctatat
      541 taaggtgctt agttgtagaa attgaacctg ttttaatagg tatttttctt gcattaccaa
      601 cacttattgg atgacctaca gctccaaact ctgatgtatc tggattgata tacgttaaag
      661 tagcaaatcc agaaagtaaa ttgttaaaac taattttttc taatatgtgt ttggtagttt
      721 tcattgttat aagttgtcca cctctattta ataaaataga tacttgtttt tcttctggta
      781 aagcatttaa ggtattggca aagtcactat atccatcaac ttcagcatta tttaccttta
      841 ctatagcatc acctaatcta aatggagatg cttctccagg atttctaact atgatatttt
      901 ttaattctgc gtcaatttga agtacgtttc caattggtat taaatattca ggttcatttt
      961 tttgtaaatt ttgtgcataa attaaattat ttgagaaaaa atataataaa aataaaaatg
     1021 ttaaaacaat tgtaaatttt gacttagcta agttggtttt tttacttaaa gtattatttt
     1081 taaaatgaaa atttttaagt tgcataaaac acctctttct tattaaatta ttttaataat
     1141 agaattgaag ttttcttgct gcttctatat tatgttgtgc aagaatcatg ttttgatgct
     1201 tggaaaacta gctcaataac accttagttt tcataaaaat aaataaaata aatagtgtat
     1261 atgataaaat aaatacatat tcatataaat aaaaataaac aataactgtc tagcataaga
     1321 aaggagcaaa aaatgaaaaa atatagattg ttagttactt gtataattgc catgggaatc
     1381 ataattatag gaacatcaaa tccagttagc aatttttaca agactacaaa tttgattagt
     1441 tcaggaaata tcacaaatga aaaagcagat aaagatgaaa attcaaacaa aaataataaa
     1501 gataaaaatg aagacaaatc taattctaaa agtaataaag ataagtcatc agaggacaaa
     1561 tcgtcagata ataaaaatga aattcaaaaa aataaaaaac ttttaatttg tatagaccca
     1621 gggcatcaag gaaaaggaga tagcaattta gaaccagtag ctccaggttc ttcctctaaa
     1681 aaagcgagag tatcttcagg aacagagggt attgctacaa aaaagccaga atatgtttta
     1741 aacttagaag catctcttgt tttaaagagt atattagaat ctaaaggtta taatgtaata
     1801 atgactagag agacacatga tgtaaatata agtaattcag aaagagcaat acttgctaat
     1861 gacaaaaaag cagatatggt tgtaagaata catgcagata gtttaaataa ttcttcaaaa
     1921 actggagcat ctatattgat tcctgaaaaa gatggaaaat atacagctcc tatatatgaa
     1981 gaaagtaata aatgtgctga atttatcaaa cagaatatgg aacaatcagg tatacaaatc
     2041 aatggaatcg ttcaaagagg tgatttgact ggattcaact ggtcaaaagt accagctgta
     2101 ttagtagaaa tgggatttat gagtaactat aatgaagacc aaatgatgtc aaatccagac
     2161 taccaaagaa aaatgatgca gtgtatagct gatggattgg atgcgtattt taaataagat
     2221 atgaatagag aaaaagttag gttggtatta ttttcctaat ttttatctct attttttatt
     2281 gtacataaca caaagaagaa gtttatttta ggttaaattg tcgtaaatat aactaaaaat
     2341 attgggaata cttgatttaa gtttaaaaag aatatattat agtatagaat gtgtaaaatt
     2401 tttttgagag gtgttgaaat gctagtagta gaaaatgtga gtcatggatt tggtgcgaga
     2461 acaatattag aaaatgtatc ctttagactt agaaaaggtg aacatattgc actagttggt
     2521 gctaatggag aaggaaaatc gagtttttta aatataataa caaagaagct tatgccagat
     2581 gctggaaata taaaatggtc ttcaagagct acagtaggtt acttagacca acatacggta
     2641 ttaagcaagg gaaaaactat aagagaagtg cttagagagg cttttaagca tatgtttgac
     2701 ttagagcaag aaatgattgc tatgtatgac aaaatgggag aagctagtga tgatgaaatg
     2761 agtaagcttc ttgaagaaac agcagagata caaactatac ttgaaaatag tggtttttat
     2821 atgatagatg caaagataca agaagttgca aatggtcttg gtcttggaga aattggtctt
     2881 gataaagatg taactgactt aagtggaggg caaagaacaa aagtacttct tactaaactt
     2941 ctacttgaaa atccaactat tcttatattg gatgagccta caaactattt agacgaagag
     3001 catataacat ggcttactaa gtaccttcaa gagtatgaga atagttttgt cttagtttct
     3061 catgatattg aatttataaa taatacatgt aatgtaatat atcatatgga aaatggagag
     3121 ttaaatagat ataaaggaaa ttatgatgaa tttgtaagac ttaatgatat aaagaaacgt
     3181 caagaagaac aagcttatga taaacaagtt gaagaaagaa aaagactaga ggattttgta
     3241 gctagaaata aagctagagt agcaactaga ggaatggcta acagtagaca aaagcaactt
     3301 gataagatgg aaatattaga aagaccaaaa gaaaaaataa aacctacttt tgcttttaaa
     3361 gatgctagag cagctagtaa aataattttt gaaacagaag atttagtttt agggtatgat
     3421 gaagcattga caaaaccact taatttccat ttagaaagag gaaaaaagat tgccttaaaa
     3481 ggaatgaatg gtattggaaa atctactcta ttaaaaactt tacttggaat aataaaacca
     3541 tttgaaggaa atgttaaatt aggtgactat ttggaagttg gatattttga acaagagagt
     3601 tcaagagaaa atagtaatac acctatggat gaagtttgga gtgaatttcc agggcttaca
     3661 aactttgaag ttagacaggc tcttgcaaaa tgtggactta caaatgaaca tataacgagt
     3721 caaatgagag tattaagtgg tggagaagct gctaaggtta gactttgcaa agtaatgtta
     3781 aaaaatataa atttcttagt attagatgag cccacaaatc atttagatgt tgaggcaaaa
     3841 gatgaattga agaaggctat caaagagttt aagggaacag ttcttttagt atgtcatgag
     3901 ccagaatttt attcagaaat tgttgatgat gtatggaata tagaagattt tacaactaaa
     3961 atcgtgtagt gatttaactt attgctgggg ggatttcatg aaaaagtttc taaatgtact
     4021 tttttcagtt tgtattagta ttgttataat agtaggtgtt attaatttta ctgttggatt
     4081 taaacaactt tattattttg acattgatta cttaaatata tcagagttat ctggtttatc
     4141 taaagatgat ataaaattga actacgatta tctaattgat tataacttaa ataagaatgt
     4201 gtctgagttc aaattaccaa cattaaaatc ttctccacaa gggaagatac attttgaaga
     4261 agtcaggaat atatttcaaa atataaataa attagctaaa ttattattgg tagtttcttt
     4321 agtaggaatt atattgagtg taaaaaataa aaatataaaa atcttaaaaa ctacatcaat
     4381 aacacttata attatgccat tattattaac agttccaatt ttattaaatt ttgaaaagtc
     4441 atttattatt tttcataaat tattatttag aaatgattat tggatattta atcccgattt
     4501 agacccagtt ataaatatat tgccagagga attttttttt cactctggaa tgatgatttt
     4561 aatattaatt ttgttagtaa gtatattact gtttgtaatg tataaattat gtaaatcttt
     4621 atcaataaaa atatttcaat gttaaataga gggatatttt tatttccaaa ataagataat
     4681 aaaaaaggat tagattaaaa gttatacaat aaaaatagct gtgcaataat atttagacta
     4741 tgaaagtagt taaaaaatat ttatgtacag ctatttataa tagaatttac tttttactag
     4801 tgattttaac taaatcttga aatttactca taatgtaatt gtaattatct tttttgtgag
     4861 gtaacatgca actactgcaa tctttgatac ctttatctgt atacttaaag tttccaccac
     4921 agttttctcc taaagcatac aaaggacaat agcaaaatag acagttaaac tcttctggtt
     4981 tattagtttt atgacaaggg aaaaattcgc aatctttgtg attaaaaaac ttataatttt
     5041 cgcccatatt ttcctcctaa ttaaataaaa tttaaatttt ttatactata taagattttg
     5101 gtgataatat aattatacta aataatttta tgaatagtcg ataatatagt attattaatc
     5161 atagtattaa tggtataatt agtcaacaga aaaataatgt aaaaataatg taagtgtatt
     5221 ttgtaattag aatatgtttt ataacaaaaa atgaggtgat taagtgaaag aaattcaaga
     5281 aaaagcactt cttgtaggtc tgaatttgac tacaatagtg aaaaaaaatg atgatataga
     5341 cactaatgaa tctatggaag aattaaaaga actaacaaaa gctgctgggg cagaagttgt
     5401 aggtagctta attcaaaata aacactctgt agatgcagct tattatatag gaaaaggtaa
     5461 agtagaagaa ataagagcct atagtgactc tttagatgct actttagtaa tttttaatga
     5521 tgaattatct ggtgctcaaa tcagaaatat agagaatgtt gttggaagaa aagtaataga
     5581 tagaactact ttaattttag atatatttgc tcaaagggca cttagcaaag aaggtaaatt
     5641 acaagttgaa ttagcacaat taaaatatag attgccacga ctttatggaa tgggtggtga
     5701 gatgagtaga acaggtgcag ggattggtac tagaggtcct ggagaacaaa aactagaaat
     5761 agataagaga catatactta ataaagcagc agatataaga cgtgaactta aagaagtaaa
     5821 aaagaataga gagacacaga gagttaagcg tttaaagtca aatataccga ttgtagcact
     5881 tgttgggtat acaaatgcag gaaaatctac cctgttgaac gaattaataa agacacacaa
     5941 ggattatgaa caagaaaaag aagtttttgt aaaagatatg ctttttgcaa ctttagatgt
     6001 aactttgaga aaggctcttt tgccgaataa aaaagaattt ttagtagtag atactgttgg
     6061 atttgtaagt aaacttcccc atgatttagt agaagcgttt aaggcaacat tggaagaagt
     6121 acagtatgca gatttaatct tacatgtaat tgatgcaaca aatactagtt atgaattgca
     6181 aaaaagtacg actgaaggtg tattaaaaga acttggtgta aatgataaaa aacatatact
     6241 tgtatataat aaagtggaca agctagaatt ggatatatat cctaaaagtc aagaagatat
     6301 tgtgtatatt tctgcaaaac aaggcataaa tatggataag ttattaaata tgatagaaat
     6361 tgctttaatg gaaaatactt attcagtaag tttgatgtta ccttatgaga gaggtgatat
     6421 atttagtaga ataaaagata aatataatgt ggagaatttt gagtatgggg aaagtggtat
     6481 aacgctagat gtagatttag atgaagaaga ttttaatatc tatagagagt acatactaga
     6541 aaaataggtt gaatatggat ataaactggg aaaatataga taatcttgaa gattatttta
     6601 taacctatct tctttataaa gaatctaaaa cagtatctca aattagtaaa attagaaata
     6661 tttcaagtac tgaagcaagt gagcaactta tacaagcaaa gttaaaaata aaggaaatgc
     6721 aaaaagatga ttttgaggca tctaaggata ttttggataa atttctagaa ctagataaaa
     6781 ttaagagact tgattttatg gatagcttag atgacgaaaa aatggtatac tttaagagaa
     6841 aggttttcaa aagaatcctt gtagaaaaaa atgctgaaga tttaattgtt ttaatttggg
     6901 cgacaggtga gctaaaagat gataggtttt tgaaattgct tcatcagctc acaaaccaca
     6961 gacactcaga tattagaaga ataacttatt cagcaatcag aaagattgaa tcaccaagta
     7021 gtagagaagt actgcaaaaa ggtctctacg acaaaaatgc acagactagg cagtactgtg
     7081 caaaagctct ttcaaaattg ggagatgaaa acagtttaaa gattttacag cagttgaaag
     7141 acaagaataa gaattttgaa aaagaatatg tgcttagagc ttatgacgaa gccattaagt
     7201 cattggaaaa tgcaaaaaat ttgaaaaagt aattttttat taaagcaatt tgtttactta
     7261 atgatgtatt tctaaagttt aacaaggtaa taaataaatt aatgggaggg gtttcaaatg
     7321 atagtaagac gttgtgcagg tggagtagtg ttttatgcta ataaagtact tattgtaaaa
     7381 aatgataggg gtgaatggac acttccaaaa ggaaagatac taggaggggg acttccatat
     7441 gagagtgctg ttcaaagagt taaagtagaa actggcatag atgctaaaat gatagatgtg
     7501 gctggcgata ctatgtatga atttttctca agaagtagac aacaagaagt ttgtaatgca
     7561 ataatgtggt atgttatgga agcttgtaac actgattgtg tacttgctcc tgaatttcaa
     7621 gaaggtggat tttataaagt aaaggatgct ttagaaatgt tatcacatca taaagagcaa
     7681 gctttagtag aagtatctta taaaaagttt aaggaattaa agaaattagt ttcagagggc
     7741 gatgatgttc cagcaaatta attgtgaatt tagaggattc tgaggaaggg atagcccctt
     7801 ccttttttaa ttgaagcagt tttaagcgta aattatatag tctctttaaa ctatgattta
     7861 tcaatttaga gatttagtag tttatattta aaatcagtaa gatatattta aaataaaaat
     7921 taaaaagtat acaattttat tatataattg tcttatgatg ttgattaaag aattattaaa
     7981 agtccattta tttttaaata gaataatttt ataaatatat atttgatgtg tatattagaa
     8041 aatgattaaa ataggaggaa atacaatgag caattacagg acattacatg aatttggaac
     8101 ggatgaaata acgattgaaa agtctgtttt tattggatat gctaagccta taaagagtga
     8161 agaagaagca ttagagttta taaatgaaat aaagaaaaag cataaagatg caactcacaa
     8221 tgtgtgggct tatactgtag gtaaaaatat gaatatacaa agatatagtg atgatggaga
     8281 accacaagga acagctggga tacctacatt agaggttata aaaaaagaag atttgagaga
     8341 tgtagcggtt gttgttacta ggtattttgg aggaacaaaa ttaggtgcag gtggtctagt
     8401 tagagcttat acaaaaggtg ctaaactggg acttgaagca ggtaagataa tttataaggt
     8461 aatgtatcaa gaagttaagg ttaagataga ttatactcaa cttggaaaag tgcaaaatga
     8521 gttaatgaat ttggggtatt ttataaaaga tactgtatat gaggataatg ttgaaatagt
     8581 agtatattca agattagaag atgtagagaa attatcagaa aaaatgattg atataacaag
     8641 tggaacagga aaaatagttt taggagagga attttatttg tctgaacaaa atggagaaat
     8701 tttaatataa tctaaaaggt tattagaata gttaatacct caaatagcca attagtattt
     8761 ttaagtgaaa agccattaaa ttgatttaat ctaaatcaat ttaatggctt tttatcggat
     8821 tttgcacaga atcctttttt tacttcaaat aaaccgacta aaagtaaaaa tatcccaaat
     8881 atttttttta atatatctga agacagataa ttagctaata gtgaaccaaa tacagctcct
     8941 aaaaccccaa acacagctat aggtataacc aatttaaaaa ttacattttt attttttata
     9001 tgtatagcaa gtgctataag tgtcataggg atagatgaaa gtaaatttac gctttgtgct
     9061 attttagtgt ctatgccagc aagtaaaacg agtgcaggta taaggattgt tcctccaccc
     9121 attcccattc ctccaataat tcctgcaaaa aatccaatta gagcaaataa cattaaaaca
     9181 ccatccttaa tgctgcaatt atcataatta ttccaaaaga aattctaaga aattttccag
     9241 taaccttatt tagtaattta gctcctataa taccaccaag aatacttcca actgcaactt
     9301 taattgttaa gttaacatca taagtacctt tagatacata taaaacagaa cttgctgtcg
     9361 ttaagaatat tattatagaa agagcagttg catgagattt atgttcttct accttaacga
     9421 tgtcatttaa tattggaact aaaagagtac ctcctccaga accaaaaact ccatttataa
     9481 atcctgtaaa aactccaatt atcgtatttt ttaagttaaa atcaaagtat tttttcttta
     9541 aatcatcagt ttttttcata aaaatcacct tctgaaatag ttttaccaaa ataggcaaat
     9601 tatatttata taaaataaga ttaaatataa tctttcatgt ttgtttaatt ttgcataaaa
     9661 aaaaacatat taaaacaaca tattaatata tggtataata tattaaaaca aagtagcaaa
     9721 tttgagagga gataacaatg tcaaatataa aaatacaaat agacaaaact gtagaatggc
     9781 ttataaataa ggttaatgag gcaaatgcaa aaggattaat tgttggtgta tctggaggaa
     9841 ttgattcggc agtagttgct aatttaatta aaaaggcatt tccagaaaac tctatgggag
     9901 ttataatgag cataaaaagt aatcctcaag atagagaaga tgcacttaag gttatagagg
     9961 gatgtgatat agaatacctt gatttagacc ttatagaacc acaaagtgct atattagaca
    10021 tggtagtagg aaatttaaaa gataaacatt tatatagaga agaatattta aagatgacag
    10081 atgcaaactt aagagcaaga gtaagaatga gtacaattta tacaattgct aacaatctag
    10141 gatatctggt tgttggtaca gacaatgctg cagaaataca cacaggatat tttactaaat
    10201 ttggtgatgg tggagttgat attttaccta ttgcaaattt aactaaagga gaggtgtatg
    10261 aatgggcaaa agagcttggt gttcatgaag atttaataaa caaagctcct tcagcaggtc
    10321 tttgggaagg tcaaacagat gaagatgaaa tgggaactac ttacaatatg atagatgctg
    10381 tgttagaagg tagattggat gaagttccta aaagagacca agagataatt gaaagacttc
    10441 atagattaag tgaacacaag agaaaaacgc ctgcacaacc tcctaagttc taatttacta
    10501 tttataagat ttgtgataaa atttacaagt gatgaaaatt agaataatta attgtacgta
    10561 ttaaggagtg gaaatatggg acgtataggt aatataatta atagaaaagg taaacaagat
    10621 gctcaaagag caaagatatt tactaaacat gctagagcaa tagcagttgc agctaaagaa
    10681 ggtggagcag acccagagta caatgcagcg ttaaaaacag ctatagaaaa agctaaggca
    10741 gataatatgc caaatgataa tatagataga gctatcgcta aaggtgctgg agcaggtgct
    10801 ggagaagact atgaaactat agtttatgaa ggatatggac ctggtggagt tgctgttata
    10861 gtagaaactt taactgacaa caaaaacaga actgctggta acgtaagata ctattttgac
    10921 aaaaacggag gaaacttagg aactagtgga tgtgtgtctt ttatgtttga taaaaaagga
    10981 caaatattag ttggtttagg agatggagtt tcagaagaag aattgatgga tgttgcatta
    11041 gaagcaggag ctgaagattt cataacagaa gaagatggtt atgaaataat aactacacca
    11101 gaagattttt ctagtgttcg tgatgagtta aaagctaaag gatatgagtt tatatctgct
    11161 gatgtgaaga tgatacctca aactacaact gtattgactg aagaatctca cttaaaaatg
    11221 atgaataaat tagttgatat gcttgaagaa gatgatgatg ttcaagatat atatcataac
    11281 tgggaagtag aataaatagt ataagcatat tgaaatttat aagtatttaa tcaattttaa
    11341 catgtgataa attttataga ataagaaaca ctaaaataga catacgcacc tctaacctat
    11401 atttgcataa taatatgtaa gcataggtaa ggggtgtttt tatatttata ggttaagaaa
    11461 tttaaagtaa attttgagct ggactcgatt gtctataaat aattgatggg tatataactt
    11521 gttgctgaat accatttata cgcccagtta ctgtatcaat aatcatccta agactacgtt
    11581 ctgctgtctc ttccattttt aaatctataa ctgtcaaagg aggaatactt cttgccatta
    11641 gtcgtgaagg tccattactt gctgcaaata tttgaatatc ctctccaatt acaattcccg
    11701 attcatgaaa tgcattcatc ataccaagag ctaccacatc atgattacaa aaaattactt
    11761 tttttagtga attttttgca agcatttttt gagcaatatc atatccacta tctattttat
    11821 tagaacaata atatatagaa tctgatagat gtattccatg ttctttacat gtttttaaaa
    11881 aagatttacc ccttaaagtc atacatgtaa aggaagttgg gttgaataca acagcaatat
    11941 catttttacc attttgaata gcatgtaaag cggctatacg accagtttcc tcattatcaa
    12001 tagatacact tgaaaaacca ggtatttcac gatttacaac aactacagga attttaggtt
    12061 tattttttac tagatattct atatcatcag cattgatacc tgttaaaaca attccatcat
    12121 aataattatt tgcaaaaaag tattcatcat cacatagatg accaactttg taaggtcgta
    12181 tgatgatatt tatgtttaaa tgttctaaag aaattatgtg gtttattgct tgaagtatat
    12241 tggttgatgc caactcaaat ccctcatcct gccaataaaa agctatttta atattattat
    12301 gtttaatagg agtagagcgt ttatatccaa gcttatcagc tgaatttaat ataaactctt
    12361 gtgtatgtag cggaatcctg cgttcatttc ctttaccact taagacgtac gagacagttg
    12421 ctgaagaaag accacagtct tttgcaatat ctttgattgt tgccatgata aaatcctcct
    12481 taaatagttg cttaaatttg cttataccaa taatcttaac atatattcaa ctaaagaaca
    12541 atattctaca aggtgcatat tttacaggat tagaataaat aaatttaaaa aaacttctta
    12601 cttatgttga cataatttag tttataggat tataatatgc ttaaataagc ttattattaa
    12661 cttgaattaa gcaaagttaa gcaggaacat gcaatgctag cttgcaatgt gactgtaaag
    12721 ggggaaaagg tatgagtaag gtaaatatat ttgaagttgg tcctagagat ggatttcaaa
    12781 acctgaaaga gtttttagaa actgataaaa agcttaagat tattgatggt cttattgctt
    12841 cagggattaa acaacttgaa attagttcat ttgtaagccc aaaagcaata cctcagatga
    12901 aagatgcaaa agaagtagca acttattgtg tagaaaaata tcctgatgtg aaactttatg
    12961 cattagttcc aaatttatac ggtgctaaag ttgcatggga gtgtggtata agagatatat
    13021 cctatgttat atctttgagt gaaactcata ataaaaaaaa tataaatcgt acccatgaag
    13081 agtctattga tgagcttaaa aaaataatga atacttatcc agacatgaat atatgtattg
    13141 gaatggctac tgtatttgga tgtccttttg aaggtttacc aaaaattgaa gatgccataa
    13201 gatttgctaa acgcctttgg gaaaacggta tcagggagat gaatctagca gatacaatag
    13261 ggattgctga ccctaagcaa gtaagagaaa ctattagtgc attagttgaa gctttaccag
    13321 agtgcagatg gcaggtacat attcatgata ctagaaatat gggaatggtt aatactcttg
    13381 tggctattga atctggaatt actactgttc agtctacact tggaggatta ggaggatgcc
    13441 catttgctcc tggagcatct ggcaatactg caactgaaga tttagtatat atgcttgaac
    13501 gtatggggta tgatacagga gttgattttg ttaaacttct tgaattatca aagtatcaga
    13561 agagttttgt aaatggagta tatagtggtc atcatgtaaa tataaagtct gatagttgta
    13621 attgttagtt ctattgatta atcgattaaa aaaatatgtt gattaaaaaa atatgctgat
    13681 tggtaaaata gatttattga taaaaaatat aaattataaa atacattatc taataaaaca
    13741 aataaaaaaa ttgaggggga ttagtatgga tattatagct ttacttttat ttttaggagt
    13801 tattattatt gcatttgtac gtaagaataa tgcaggtatt ttggctctag ctattggtgt
    13861 catagctgtt agaatttttg gaatggatga taaagaactt tatggagcta taagcaactc
    13921 aatgtttact acacttgttg gtataacact actctttgca atagtgaact caacaggagc
    13981 attaaagctt gttgcagaaa aaattgtagc tgcttctggt aaacgtgttt ggcttatacc
    14041 tatagcagtt tttatagcag gatttgttgt tgctggtgtt ggacctgggg caattccagc
    14101 acttgcaatt atcccagcaa tggctgttcc tgtagccata acggttgatt ataatcctgt
    14161 aatgcttgca cttataggtg aatgtggatt aatggcaggt cgtatgactc ctattactcc
    14221 agaaggtcaa attataacaa ccgttgcaga aggggttgga attgaaaatg ttatgcctac
    14281 tatcttagcc tgtcaaactc tttctacaat tgtattttca atagtattgt ttataatttt
    14341 caaaggatat aaacttaaaa aacctataaa tgttatagat agtaagcaaa tagaaaaatt
    14401 caatataaag cagataattt ctctaataag catagtagtt atgattgctt ttatcataat
    14461 ttttgatatg aatgttgggc ttgcagcttt tgcagtttca gctgtactta tttttttcaa
    14521 tattgctgat gaaggagaat gtatcaaatc aattccatgg agcacgatag taatggttct
    14581 tggagtaggt gcaatgatga caatcgtaga tgcagcagga ggtattgact tgatgagtaa
    14641 tgcattatca tcactgatga atagtaaaac agctactcca attatgagta tttcagcagg
    14701 acttatgtct atggttagtt ctgctttagc agtagtttat ccaactatga tgcctatgtg
    14761 tgttgatata gctaaggcag ttggaggagt agacccacta gcacttattg ctgcagttgg
    14821 tgttggaggt tctcttgctg gtgtaagccc actttctact ggtggggcat tgattttagc
    14881 agctatgggt tcaagtaaga aagactttaa taaggaagaa caaaataagg tttttgtaca
    14941 gcttttcatt atgagtgcag ttggcttatt ggttattgca gtagtatcaa tattatcatt
    15001 taatgcaata acacatttaa tgaactaatt agaataactg aaaataaatc attaaattta
    15061 acttttaata ttaatcaata taatataatt tggaggggaa gttatgaaaa aaggagcatt
    15121 aagcaatata aaggtacttg accttacaag agttcttgca ggtccatatt gtacaatgat
    15181 gttggcggat tttggagctg atgttataaa aatagagatg ccaagaaaag gtgatgatac
    15241 aagaggtttt ggaccttttg taaatggaga gagtttatac tatgcaaata taaatagaaa
    15301 taaaaaaggt atcactctaa accttaaatc agaagaagga aaaaatatat ttttagaaat
    15361 ggtaaaacat gctgatgttg tagtagaaaa ttacagacca ggtgtaatgg aaaagttagg
    15421 tctgggttat gatgagttgg ctaaagttaa caatcaaata atttatggag ctgtatcagg
    15481 atttggatgt tatggaccat attcagaacg tccaggttat gatattattg cacaggcaat
    15541 gggtggtctt atgagtataa cagggcaaga aggaggagaa ccaacgagag ttggtaatgc
    15601 tatgggtgat gtactaggtg gaatgaattt aactattggt atactcacag cactcaacgc
    15661 tagaaaaata ataggaaaag gacaaagagt agatgtttcc ctcgttgatt ctgttgtatc
    15721 gtctctcgaa actggtatac agagatattt tgattcagga caacctgaac ttatgggtaa
    15781 tagatatgct tcagcctatc cttatgactc ttttaaggca agtgatggaa gttttgtaat
    15841 aggctgtggt aatgacaaac tttttgaaaa gttatgtaaa aaagttcttt ttagagaaga
    15901 ccttttaata gatgagagat ttaaaacaaa tcttaatcgt tgtgaacatt acaaggctct
    15961 taaaccagaa atagaaaaat ggtctgttaa gcatacaata aaagaagcag ttgacttaat
    16021 atcagaggct ggagttcctg ctgctcctat ctataatctt gattgtgtta caacagatga
    16081 acatatagca aaagcaagag aaatgattgt agatattagt catcctatta taggaaatat
    16141 gaaagtcaat ggaaatccag ttaagttgat gacaacaaaa gctaaaattg atatgccagc
    16201 accatctcta ggggaaagta acgaggagat ttataatggt tggcttggtt ttgatagtga
    16261 taaaataaaa gatttaaaag aaaatggagt tatttgatta aaaaatagct aaacacactt
    16321 gtgactttag tcatgggagg ttcagttaga ataatatcaa aaaatattaa atcaatactg
    16381 taaataaaat atagaattgt cagaagcatt taagatggga aaagctactt agacattgag
    16441 tctttgtggc ttttcctatt ttcattaaga attttatatt atcagaatag atatgtacta
    16501 aactaaagta ttaacaattt aaattaatag aaaataaact acctaattat gttgaataaa
    16561 gactacttta gtgaaaataa acttatagag atgctattta aattatttta atggaaatat
    16621 caaaaatgag actgcattct caaaaatgag actgcgtttt ttaagtattt tctcgaaaat
    16681 gagacaaaaa tattgtttta tgggttttat tgaagaaaaa ttcttctgtg aattacaatt
    16741 taatcaattt taaagttgaa aattattaaa aatttagtga aatttatgta tatttcgttt
    16801 attttattaa aaataacaat tttttactaa aaataaagaa cttcaatggt gattggcata
    16861 gtacttgcta ttttatatta acaagaaatg tagaaaaaat aacaatgtat aatgggggga
    16921 atatgatgga atctgtaatg gaaaatttga acaatcttaa ggtggagctt aaggacaaag
    16981 tctgtgttat tactataaat agacctaaag ctttaaatgc tcttaatagt gacactctta
    17041 gggaactatc acaagttatt gatgtagtat cagaaaatga ggcaatatta ggagttatta
    17101 ttactggaga aggtaaagtt tttgtagctg gtgctgatat acgccagatg caaaactata
    17161 aaagtgagga aggcagaaaa tatgcaggtt atgctcaggg aatttttgat aaaattgaag
    17221 ctcttgaaaa aacagtaata gcagcagtta atggatatgc attaggtggt ggatgtgaac
    17281 ttgctatgag ttgtgatatt agaattgcat cagaaaaggc tatatttgga caacctgaag
    17341 ttaatttagg agttattcca tgttttggtg gaactcaaag attatcaaga cttgttggaa
    17401 ctggaatagc taaagagtta atttttacag ggagacaagt aaatgcagaa gaagctaaat
    17461 ctattgggct tattaataaa gtggttccaa gtgatttact tttagaagag tctatgaaga
    17521 tgatgaacca aatagttgaa aaagcgccaa tagcaataag atatgcaaaa gttgttatta
    17581 acaaagggat agatatggat ttaaagaatg ctcttgaact agagaaagat attgcaggtt
    17641 taacttttgc tacaagagat aagcaagaag gaatgaatgc atttatagaa aagagaaagc
    17701 cagtttttga aaataagtag ctattataac taaaattgga acaatgtaga atatattcaa
    17761 aatctaaatt tcgtgaaaaa atacatatag tttaatttta aaaggagtta atttatgaat
    17821 acgggagtaa ttataagttt agtagggatt atcatagctc tatctcttct agttgtaatg
    17881 gttatgaagg gaataaatat ttttattata gcaattgtat gttcatcatt agttgctata
    17941 acaggaagcc taaatgtgta tgatgcactt aaagtggatt atatgacagg gtttgttgga
    18001 tttcttcagg caaatttctt tattttccta acaggtacat taatgggaaa aataatggag
    18061 attactggtg gagcaaaatc tattgctaag atgattgtaa gatggattgg gaaggataaa
    18121 gcactacttt ctattccaat agcttgtgga atattagcct atggtggagt aagtgttttc
    18181 gttgtaagtt ttgcagtgtt tccgatagct ttagaaattt ttaaagaagc tgatttgcca
    18241 cgaagattta taccagcagc tttgactttt ggatgctcaa cttttgctat ggttgcacca
    18301 ggggcaccac aaattcagaa tattgttcca gcatcaacat tagggacaga tattatggct
    18361 ggagcagtaa atggttttat atcatgtgca gtaatgttta tagttggaag taccattttg
    18421 tacaaaatgg tttcaaaaga aaaagccaat ggtggtcatt ttatagcaca tgaatcagat
    18481 atatttgatg aagcagaagc aactaattct aagagagaaa atggtcccaa tggtgtaatt
    18541 gccttaattc cattgatagt gtcaatttta ataattaatg taaaagttaa tggaaaagcg
    18601 atagtaccaa ttgaggtggg agtatttatt ggagctttat tagtatattt attgttaaat
    18661 aaatatcaag ataatactaa aatagttgga catgttggtg atgcatgtaa aactacagta
    18721 gtagcaatat gtaatacttg tgctgtagtt gcatttggtt cagtagtaaa atcagcagtt
    18781 ggttttgact tcatagttaa tgctatgact aatattcctg gtcctcctat tgcaggggct
    18841 gcagttggaa ctacagttat tgcaggtatt tgtgggtcag cttcaggtgg actaggaatt
    18901 gcagctccac ttcttggacc agtattttta gccaagggtg tttccgctgc agcattaact
    18961 aggactatgg caatttcatc agcagcatta gattcacttc cacacaatgg atatattgtt
    19021 actgtaacaa atggtctttg taaagaaaca cataaggatg cctatatgcc aatattctgg
    19081 gtaactgttt taaccccatt tattggaacc atagtagcag tcctactgtt tacaatgttc
    19141 ccgggattgc cataagagaa aagataaaag gtataaatta tattttgata ttataaaatc
    19201 aattttcaaa atatatacaa taaatttcat gttttaatac tgtaaatatg tatttaaaat
    19261 aacaaattga ccatataagg aggaattgaa agtgttaaat aaaccattag agggagttaa
    19321 ggttattgac ctaacgtatt ttgtagcagg tccagggact tctaaaatat tagctgactg
    19381 gggtgctgac gttattaagg tagagccaag ctttggagac ccaggaagaa agacaggggc
    19441 aacaatgaca atgccaatag atgattataa taatcctttt tacagcacat ataattcaaa
    19501 taaaagaggt ttgtctataa atcttaagag tgaaactgga attgaaatta tggataagct
    19561 tttaagcgaa gcaaacgtat ttgtttcaag ttatagaaca ggagcattaa aaagactagg
    19621 tcttgattat gaatcattaa gtaaaaagca tccacacctt atctggggtc agataaatgg
    19681 ttttggcgat tttggacctg ctaaggataa tgcaggattt gatacagttg ccttttgggc
    19741 aagaagtggg gctatgcttg atatagcaga aaaagataca agtccaataa atccacctat
    19801 agcctttgga gatgcaacaa cttcttgtag tttatctgga ggaatatgtg cagctttgta
    19861 ccagcaagct aaaactggaa aaggacaaaa agtaatggca tctttatttg ggcaagcaat
    19921 atggaatgca tcatctttat tagcatctac acagtttagt gatgagtatc caaaaactag
    19981 aaaaaatgct atttcaccag taataaattc ttatcgttgt aaggatggaa agtggatatt
    20041 cttaagtatt ttagagcatg aaagatattt tgaggcactt tgtagattat ttggaagaga
    20101 agacttaata aaagaagaga aatttgcatc ttcagtagaa agtaagaaaa atgctacaga
    20161 acttattaat attttggatg gtgaatttgc aaagtttact caaaatgaga tggttgaaaa
    20221 acttactgct gctgatatag ctcatgaaaa aattcaacat gttaatgatg tagtgactga
    20281 tgaacaggct attgctaata actatatata tgaacataca agtaaaaatg gaaataagac
    20341 aatgcttgca tctactccag ttaaatttgg aaatattgaa gttaacatga cttgtgatgc
    20401 tccacttata ggagaacatt cagatgagat attgagagaa cttggataca gtgattctga
    20461 tatagaagct ctaattgaat ctggaattgt aacaatgaat aaagaagcat gtgaaatatg
    20521 ttaataaaca tgtgaagtat gttaataaat aggagggaat tttatggatt ttagattaac
    20581 agaagcacaa ttaatgcttc aaagagtagc aaaagaattt gcagagaatg aaatagcacc
    20641 aatagctgct gaaacagaca aaacaggtat ttttccaagg gagcttttta gcaaaatggc
    20701 taaaatagga tttaatggaa ttggaacacc tgtagagtat agtggttctg gtggggcaga
    20761 tatagaaaag gtaattgttg ttactgagat tgccaaaaaa tgtgcagcat ctgcagcaat
    20821 attatcaata cacacaatat atgctcaagc tattttgaaa tttggtactg aggaacaaaa
    20881 gaaaaaatac cttccaatga tggctgaagg tgggtgtgta ggtgcttttg ccttaaccga
    20941 acctaatgct ggttcagatg ctgcgagagc tgcaacaact gcaattattg atgaagaaac
    21001 tgatgaatat gttttaaatg gaactaagtg ttttatttct ggtggaggtc aagcagaatc
    21061 attaataata tttgcactga cagacccatc aaagggaata aagggaatgt ctgctatcat
    21121 tgtcgacaaa ggtactccag gtttttcaat aggaaaaatt gaagaaaaaa tgggtattca
    21181 tggttcagaa actgcagagt taatatttga taactgtaga gttccaaagt ctaacttatt
    21241 aggaaaagaa ggcaaaggat ttaatatagc tatgacttgt ttagatggag caagaattgg
    21301 agttggagct caagctgtag gaattgctga aggggcttta gaagaaagta tcaaatattc
    21361 taaagaaaga gtccagtttg gcaagccaat ttcagctttg caaggtattc aatggtatat
    21421 agcagatatg gcgacaatgg tggaatcagc aaaattatta gtatattatg ctgcagattt
    21481 gaaagctaga ggagaaaaac acactaaaga agctgctatg gcaaaataca atgcgtcacg
    21541 tactgcaaga gaggttacaa atttagccct tcaaatccat ggtggatatg gatatatgaa
    21601 ggattatcca ttagagagaa tgtacagaga tgccaaaata acagaaattt acgaaggtac
    21661 ttcagaaata cacaaagttg ttatttcaag agcagtacta ggataggagg aaaagaatga
    21721 atatagttgt ttgcttaaag caagttccag atactaatga agttaaaata aataaagaga
    21781 caggtactct aataagagat ggtgtaccaa gtataatcaa tccagatgat agaaatgctt
    21841 tagaagaagc tctaaaaatg aaagatgaac taggagcagt gattaaggtt ataagcatgg
    21901 gacctcctca agctaaaagt gctttaaaag aagctttggc aatgggagct gatgaagctt
    21961 atttaatctc agatagagct tttggtggtt cagatacttg ggctacatct acaattattg
    22021 ctgctgctat agagaaggtt ggaaagtatg atgtaatatt ctgcggaaga caagctatag
    22081 atggagatac agctcaggtt ggaccagagg ttgcagaatt tttagggata ccacaagtta
    22141 cttatgcgaa agaagtaaag gttcaagatg acaaattact agtaactcgt tatacagaga
    22201 caggggatta tttaatagaa gcaaagatgc cagttttatt aactgctata aaagagttaa
    22261 acaatccaag atatccaagt gtcaaaggaa tcttagaagc ttataataat ggagatgcaa
    22321 agattactgt attaacactt gcagatttag atgttgatac aactcaaatt ggattaaagg
    22381 gttcacctac aaacgtttat aagtcttttg taccagttaa agacaagcat aatgaaataa
    22441 tagaaggtat aaataaaaaa gaaaaagctg agaaactaat agaaatacta ttcgatttga
    22501 aactagtata ggagtgttaa gatgatgaga gcaaaagtta atcaaggtat aaatttaaat
    22561 gattataatg gtgtttgggt aataggagag caaagggaag gtaaaattaa tcctgttacg
    22621 atagaactta ttggtgaagg aagaaagctt gctgaccaat taggaaaaga gcttgcagtt
    22681 gtgatagctg gatatgaagt agagaaagaa gtgaaggaat tacttcacta tagtgttgat
    22741 aaagtatatt acatcaatga ccctcttctt aaagatttca ctactgatgg atatgcaatt
    22801 tcaattgcaa atttaattga aagaaaaaag ccagaggttg tattggttgg tgctacttct
    22861 ataggaagag atatagcgcc tagaattgca ggtaaggttg gaacaggtct aactgctgac
    22921 tgtacaaaac ttgaaataga ttcaacagat aataagttat tacaaacaag accagcattt
    22981 ggtggaaatt taatggccac tattgtttgt ccaaaaaata gaccacagat gtcaacagtt
    23041 cgtccaggag ttatggcaaa agctgtaaga aacgaatcag agactggaat tttagaagtt
    23101 gttactccag aacttactga aaaaatgatt agaactagat tagtagagat acttccacaa
    23161 gaaaagaaat ctgtaaatct aactgatgct agaataatag tatcaggagg aagaggatta
    23221 aaaagagcag aaggatttga gcttataaaa gaattggctg ataaattggg ggcagagatt
    23281 ggtgcttcaa gagcagcagt agattcaggg tggatagaac attctcatca agtgggacaa
    23341 acaggaacta ctgttagacc agaactatac attgcatgtg gaatatctgg agcaatacaa
    23401 catttagcag gtatgagtga ttctaaatat atagttgcta taaataaaga tgcaaaagcc
    23461 cctattttta gtatatgtga ctatggaata gtaggagatt tatatgaaat tatacctgaa
    23521 atgatagaat cattaaatag gtaactttta gaaaacattg aatatgtaga atgataagtc
    23581 ttataatatt gtattgtaag acttattatt tttgctgaag gttatagtgg ggagatgaaa
    23641 caatgaaaga gcagtggtat aaggatatat ttgcaagagt attatctatg actgatgatg
    23701 gttttattgt tgtaaataca agtggagtaa taatagatat taatgataaa tattgtgact
    23761 ttttaggaaa agaaagaaaa gatataatag gacaaaatat acaaagtatt ataccaaata
    23821 caaaaatgct agatgttatg aaaaataagt attgtgaaga aggagctata catcattatt
    23881 caggtggaaa tacaaaagaa aaaagtgtaa tagtaagtag gtcttatgtg gaaaatgaca
    23941 atggagaagt agttgcagga gtagctcaag ttaaatttag attgcaatca tttgatgtag
    24001 ctaaaaagtt aatgtcagaa tatatggagc tccaatatta taaagagcag tttaaggata
    24061 actgtggttt tgacaagctc ataggtgaaa atagagactt catagaactt aagaaaacag
    24121 gtgtgaaagc ttctaaaaca aactttccag tacttttaac gggtgaaaca ggtacaggaa
    24181 aagaagtctt tgctagagca attcacaata atagcagtag gtcagataaa cctatggtta
    24241 gtataaactg cgctgcaata ccagaagaat tattagagtc agagctattt ggctatgacg
    24301 aaggagcttt tacaggagca aaaaagggtg gtaaaaaagg taagttttta gttgcgaata
    24361 atggtacaat ctttttagac gagataggtg acatgccttt gaccatgcaa gcaaaacttt
    24421 taagagttct tcaggaaagt gagatagaac cagtgggagg tcttaaaact ataaagatag
    24481 atgtaagagt aatctctgcc acaagaaaga atttaagtaa aatggttgaa gagggattat
    24541 ttagagaaga cctatactat agattaaatg taataaacat tcatatgatg gaattgaagg
    24601 acaggcaaga tgatatttta ttgctagcaa attatatatt aaacaaatta aatgttgaat
    24661 acaaggaatt gaaggtttta agtgataagg ttaaaaattg ttttattaac tatacttggc
    24721 cgggaaatat aagagaacta caaaatgtaa taaaaagtgc ctatgctgta agtgatgata
    24781 tggtcattat gatgtgtgac cttccatcta agatggataa tatcagcaga gtagctcaat
    24841 gtaatgtgga tagcaattgt tcaattcatg aaatggttga gaattatgaa aagagcttaa
    24901 ttattgatgt gttaaggaag tataattgga aatgttctaa ggcagcagag gttatgggaa
    24961 tacacaagag tcttttatat aagaaaataa aaaagtatga aattgaatta aataattaag
    25021 ataaattgaa ttaaggcaat gctaaaaaag cgcctttatt caaagcgaac tgtataaact
    25081 gttttatgca gttaaaacgt gtaaaagtag atgtgaagta ctaatgtcaa tcattagtac
    25141 ttttttatta tatttataat tactatctat atttcagtat cttttgatat ctaagattat
    25201 tatgtttaat ttaggatata acaaaaccct cttagttatg gacagttttt tatattttaa
    25261 ctgtctacta agaggggata gtttagttta aatttagagt aaattttatc tagcatttat
    25321 aaaaaatatt ttgaatagct tttttgttat ttagtcataa ttttctactt ctttatcaag
    25381 ttctttgcca caagaatcac acttatatat agcattgtat ccgtaatcat attcatcaac
    25441 tattttcttt tgtctaaata caccaccaca ctcacatagg aaaccaattt ttataagtgt
    25501 aacgataaat tgtgatttta taaattttat atcattacat ttatcgttag tgtaaaatgt
    25561 ataaatataa gagttatcgc actcatctct taattcaaat ctttcaatat atgtgttatt
    25621 ttttaattcc tcaaattctt ctttagttaa atatgatttt tgttgcaatt catgtaaacg
    25681 cttaatttgg tccattattt atatcctccc aataatttta gtattttaag cttagataag
    25741 gaatctaagt tttattaaat ttatcccaag gaattattta aatcatattt agttttatta
    25801 attacaacat atataaatta cacgaaaata aaactaattg taaaaataat atcctgtgaa
    25861 aacctaaact actaacccct tactaacccg aaaactaaaa aaagaaaata tatccaaata
    25921 taagtgatat ataaataaag atgtttcata taaatttaac tt
//
LOCUS       DAMKMI010000042        25810 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMKMI010000042 DAMKMI010000000
VERSION     DAMKMI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25810
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.043"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            458..1324
                     /locus_tag="PC429_001648"
     CDS             458..1324
                     /locus_tag="PC429_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3847160.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            1566..2375
                     /locus_tag="PC429_001649"
     CDS             1566..2375
                     /locus_tag="PC429_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847161.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            2519..3583
                     /locus_tag="PC429_001650"
     CDS             2519..3583
                     /locus_tag="PC429_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF3847162.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQDIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            3611..4330
                     /locus_tag="PC429_001651"
     CDS             3611..4330
                     /locus_tag="PC429_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3847163.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            4354..4938
                     /locus_tag="PC429_001652"
     CDS             4354..4938
                     /locus_tag="PC429_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF3847164.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            4940..6289
                     /gene="pabB"
                     /locus_tag="PC429_001653"
     CDS             4940..6289
                     /gene="pabB"
                     /locus_tag="PC429_001653"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF3847165.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            6279..7022
                     /locus_tag="PC429_001654"
     CDS             6279..7022
                     /locus_tag="PC429_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF3847166.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            7056..7757
                     /locus_tag="PC429_001655"
     CDS             7056..7757
                     /locus_tag="PC429_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847167.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            7783..8355
                     /gene="folE"
                     /locus_tag="PC429_001656"
     CDS             7783..8355
                     /gene="folE"
                     /locus_tag="PC429_001656"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF3847168.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            8419..9210
                     /gene="folP"
                     /locus_tag="PC429_001657"
     CDS             8419..9210
                     /gene="folP"
                     /locus_tag="PC429_001657"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF3847169.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            9221..9586
                     /gene="folB"
                     /locus_tag="PC429_001658"
     CDS             9221..9586
                     /gene="folB"
                     /locus_tag="PC429_001658"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF3847170.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            9579..10085
                     /gene="folK"
                     /locus_tag="PC429_001659"
     CDS             9579..10085
                     /gene="folK"
                     /locus_tag="PC429_001659"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF3847171.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            10066..10851
                     /gene="aroF"
                     /locus_tag="PC429_001660"
     CDS             10066..10851
                     /gene="aroF"
                     /locus_tag="PC429_001660"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF3847172.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            11106..12896
                     /locus_tag="PC429_001661"
     CDS             11106..12896
                     /locus_tag="PC429_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF3847173.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            12914..14080
                     /gene="rpoD"
                     /locus_tag="PC429_001662"
     CDS             12914..14080
                     /gene="rpoD"
                     /locus_tag="PC429_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF3847174.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            14245..14934
                     /locus_tag="PC429_001663"
     CDS             14245..14934
                     /locus_tag="PC429_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF3847175.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            14948..16045
                     /locus_tag="PC429_001664"
     CDS             14948..16045
                     /locus_tag="PC429_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF3847176.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            16108..16680
                     /locus_tag="PC429_001665"
     CDS             16108..16680
                     /locus_tag="PC429_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF3847177.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(16740..17207)
                     /locus_tag="PC429_001666"
     CDS             complement(16740..17207)
                     /locus_tag="PC429_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF3847178.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(17593..18069)
                     /locus_tag="PC429_001667"
     CDS             complement(17593..18069)
                     /locus_tag="PC429_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF3847179.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(18189..19280)
                     /locus_tag="PC429_001668"
     CDS             complement(18189..19280)
                     /locus_tag="PC429_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF3847180.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(19273..19662)
                     /locus_tag="PC429_001669"
     CDS             complement(19273..19662)
                     /locus_tag="PC429_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3847181.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            19854..20300
                     /locus_tag="PC429_001670"
     CDS             19854..20300
                     /locus_tag="PC429_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847182.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            20564..21289
                     /locus_tag="PC429_001671"
     CDS             20564..21289
                     /locus_tag="PC429_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847183.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            21276..22520
                     /locus_tag="PC429_001672"
     CDS             21276..22520
                     /locus_tag="PC429_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3847184.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            22600..23277
                     /locus_tag="PC429_001673"
     CDS             22600..23277
                     /locus_tag="PC429_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847185.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            23278..>25810
                     /locus_tag="PC429_001674"
     CDS             23278..>25810
                     /locus_tag="PC429_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847186.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENI"
BASE COUNT        10563 a         2578 c         4571 g         8098 t
ORIGIN      
        1 ataaaaactg aacgaaaaaa atgcaaataa tgaaataaaa aaatcatgta ctgaaattat
       61 aaaaagaaaa atttcattta ggaattaatt ataaagagac aattactaaa cttgaatttg
      121 ctaaaataaa ataaattact gctaaaatag atatttagaa aaaaatacta taaatttact
      181 atattgcaag ttaaataata agcagtatca tattatgcta gaaaaagttt catttaaggc
      241 ataaatgaga tgatttgaac gtttttaaag attagaatat aaaagattgc actagacata
      301 tatgaaaaag ataatataat agatgatgta atagatgaca gttcaagaaa ttataaaaaa
      361 aatttatttg aaatattatc tattacatca ggaataatat atgtaagagt aagcttttaa
      421 aattctaaag atttagaaag attgaaggga gaaagaaatg agtgaagtta agaataccct
      481 acaaaaacat ccagttttaa gatattcaac aatgctattt gggatgttga tgacatctat
      541 aggaataaat gggttcttaa gaccagcaca tctgcttagt ggaggtgcta caggtatagc
      601 aacatctata aactatttga caaatataaa tgtaggttta ttaacatttt taatcaatat
      661 tccaatattt atcttagggt tcatatacct tgaaaaagag ttttgtattt caagtttggt
      721 aaatatgatt gtattttcat tattattagg agcaactcag gaaataagta atattatacc
      781 tatacatgat atattgcttc aaagtgtata tggtggtata ttatctggtt taggagtcgg
      841 agttgtattt agaactaggt cttctcaagg tggtacagat ataatagctg caatactaaa
      901 aattaagaag aatatagaaa tgaaagacac tgctttagca ataaatggct tgatagtact
      961 tacaggaagt tttttatttg ggttggattt agcattatac actcttatag gtctatttct
     1021 aaatgcgtat tcaatgagtt ttattaaaga tgcaatgaat taccaaaaat ctgttatggt
     1081 aatgtcaaat gaagtcgatt taatagcaga agatataatg aagagtttag ttagaggtgt
     1141 aactttttta gatgcagaag gtgcatacac tcatcaaaaa aagaagatta tatatacaat
     1201 agtttcatca aatgagatac caaagataaa agatatagct ttgaagtatg ataaaaaggc
     1261 ctttatatct gtaaatgatg ttacagaagt aaagggaaga gggtttaaag caaaagattt
     1321 atagaaaatt ttacaagctg ggagtttata atgtaaattt gtatattata tctctcagct
     1381 tatttaaatt tagtatacga aaataaaggt tttattaatg atttgtgtat cactttaaaa
     1441 gatatcattt taaaattaaa attgaaaaaa ttacttaata tgtagaaaac atgaccacta
     1501 ttaggatata ataaatttaa tagatttagt aaaataccta ggaataacgt tggaggaatg
     1561 gcattatgaa tttatctaaa gaatatgtta atttgtatga taattatttg aaatatataa
     1621 atgaagtaaa caaagagatt agaagtataa agaacatgaa gaaacgtgtt ttaaaaaggg
     1681 gcgattttgc tccggaaaat gaagtattag aattggaagg aaaaacaata cgcgatatag
     1741 atgatgtcga tacaaaaaaa ccaaaaaata aaatctataa attttcaaat ggggatgtat
     1801 atgtagggaa attcttagag ggaaagatgg aggggaaagg ctcatatact ttttttgtag
     1861 atgaaggaac agttatggag tacataggag aatttaaaga agataaaaaa aatggaaaag
     1921 gaaactttat attttcaaat ggaaatgaat atataggtta ttttgaggaa gatatgatga
     1981 gcggtattgg aaatatgtta tatagtagta aagatgaata tattggaact tggaaaaacg
     2041 gaaaaaaaga tggaaaaggt atttataagt ggagtgatgg atgtatatat gcaggtgaat
     2101 ttaaaggtgg aaagatggaa gggtacggta tttgctataa tagtaaaggg gaagtacttt
     2161 atgaaggaga atggaagaat aatttgattc atggtaaggg aacttatata tgggaaaaag
     2221 gtaaaaagta tataggtgaa tttatgcatg gtaaaaaaca tgggcaagga actttttatc
     2281 taaataatga attggtttat gaagggactt ggaaatttga taagccatct atatttgata
     2341 gaacattaga tgaaatattt tccttgagac tgtagcttta tctaattagt agataaagct
     2401 tttttctaat acttactgta aataggtatg aatgatattg attagtatac actatcaata
     2461 ttaacttaat aaatgcaata ttaaaaaatt ttgattgatt ttaagaagga gagatatatt
     2521 gaagagatta attgctttag tgttagtagt acttattgtt ttattttata gtcctgaatt
     2581 attaaaagaa ggatatgatt taaaagaaaa gttttttcaa agtgaaatat ggaatgaatt
     2641 tttagacaaa ataaatataa atgataattc taagaaagat aaaaaagaaa aaagtcaaaa
     2701 tttacaatca aataatactg aaggggagaa cttaggagag tcagatataa agaattttga
     2761 aaaaataagc ttaggagata atttaagcta tgttttatca agtatcggca aacctggtag
     2821 aattgatatt agtgaatatg gttttgattg gtatgtatat aatcaatatg gaaaagaatt
     2881 tgcaatggtt ggacttgaaa atgatgaagt agtagcactt tatagtaatt ctattaattc
     2941 atgtgaaaat caagatataa aactaaatca agatagacaa acagtgagaa ctaagattac
     3001 accattaaaa tataagagaa aaggaaatac cagatatata ataaactcag aaaatcaata
     3061 tgatataata agtaaggaag gtaagtatat tacaattttc tacgatattc atgaagaaaa
     3121 tagagtttgt tcatatttaa taattgataa aagcactgaa gatgagttta aaaatttata
     3181 tccagatgat agcgaagaac ttaaaaaatg ttttgaactt gaagttatag accttgtaaa
     3241 tagtgttaga aatcaaagag ggttaaactc attgagatac agtgaacagg caactttgag
     3301 ttcaagaaaa catagtgaag atatgagaga taataatttt tttgaccatg taaataagaa
     3361 gaatgaaact ccatttgaca gaatgaaaag agaaggtata gtatatactt cagctggaga
     3421 aaacatagca gcaggacaga ttaatgcaat atatgcgcat gaagcttgga tgaactctga
     3481 aggacataga aaaaatattt tggggaacta taataatatt ggagttggag taatatttgg
     3541 aggaagctat aaaacatatt acactcaaaa tttttataaa taactaaaat atttgaaagg
     3601 aagttaaaaa atgacatcat ggaaaaaaaa gacagtatat aagtgcttaa tagcagtagc
     3661 cttgttttgt ggaatagtgt taatatctaa ttttagtaaa gtttctgctt tgatgatgga
     3721 tactaatgga aatgttttga ttaaacatgg ttcaagggaa aaaaagctaa tagcaattac
     3781 ctttgatgat ggtcctcatc ctaaagaaac tagtcaagtt ttagatgtat taaagaaata
     3841 caatgtaaaa gctacttttt ttatagctgg aaaacatgca aaatggtata aagaaccatt
     3901 agtaagagca agtaaagaag gtcacgagat aggaaatcat actttcaatc atcctgatat
     3961 aagtaattta agttctagtc agatagaaga ggagattgtt aagtgtgaag acattttgaa
     4021 ggaagtaact gggaaaaaac ctacattatt tagaccacca ttcggaagtt atagagaaaa
     4081 agacttaata gaaattgcaa aaaagcatga ctataaagtt gttttatgga caggtgtaga
     4141 tgtgaaagat tggaaaaatc caggagctaa tagcattgca gataaaataa ttaataaagt
     4201 acaaaatgga gatataatac ttttgcatga ttatgcaact aatgatacag ttgaagcttt
     4261 agatatgttt attccaaaaa tgattgaaaa aggttttaaa tttgtaacag tttctgagtt
     4321 gataaaataa tttttagggg ggagatttac agtatgattc ttatgataga caattatgat
     4381 tcttttgtat acaacctagt ccaatatata gaagaattag gggaagcagt tgtagtaaaa
     4441 agaaataatg aaatcaaaat aagtgacatt gaagaactaa atccagaggt aattgtgtta
     4501 tctccaggac cgtgttctcc aaaagaagct ggtatttgca tagatattgt tgagcatttt
     4561 aaggggaaaa aacctatact aggaatttgt ttaggtcacc aaactattgg acatgttttt
     4621 ggaggagata ttataaaagc acaacagcct gtgcatggaa aagtatatag tatcaaccat
     4681 actaataaag gagtttttag gggacttaaa aatcctttaa atgtcactag gtatcattca
     4741 ctaatagttg actccaatac agttccaaaa gaattagaaa ttacagcaat tactgataaa
     4801 ggggaaataa tgggaattag gcataagaaa tatttaatag aaggagtaca gtttcatcca
     4861 gaagctattt tgtcagagta tggtcatgaa atgcttaaaa attttataac agaagctaga
     4921 gaaagggtac atatgtaata tgataagaga gataaatact aaattaaatt cttttgagat
     4981 atttactatt tttagaaatg aacatgacag ttttatatta gatagtgcta tggataaaga
     5041 aaaattggga agatattctt ttataagtag ccaaccattt aaggtattaa agtataaaga
     5101 tactgatgaa aatcctttag aagttttaaa agaagaacta cataagtata gggttgtaaa
     5161 tgatactaat cttccatttg taggaggtgc tgtgggatat ttatcttatg atttaggtaa
     5221 ttacatagaa aatttaccta gaactgctgt agatgatatt gagatgcctg atatgtattt
     5281 tggattttat aatcatgtaa tagttataga ccatcttgtg caaaaaactt atatagctac
     5341 tccaaacata gatatcgaat tagaagaaaa gataatagat gatatagaac aaagaatatt
     5401 aaaagaagag aaaaaaggta tagatagcat atgttatgaa gaaaaagaag taacacctat
     5461 aagacttaaa tctaatttta caaaggaaga atttaaaaat gcagttcaaa gtgttagaga
     5521 gtacataaga caaggagata tatatcaagc taatttaaca caaaggttca gtggagaaac
     5581 agaacttaca agctttgaat tgtataggga cttaagaaga tttagcccag caccatttgg
     5641 ggcattctta aactttgaag atgcacatat attatcaaat tctccagaaa gatttataag
     5701 atgtgttaat aagagaatag aaactagacc aataaaaggt actcgtccaa gagggaaaga
     5761 taaagaagaa gatttaagac ttcaacaaga acttagaaat agtgaaaaag atagagctga
     5821 actacttatg atagtagatt tagaaagaaa tgatattggg agaatatcta aaactggaag
     5881 tgttaaagtt ccagagctat ttgtaattga accatatgca aatgtaaatc acttagtttc
     5941 cacagttgta ggtgagttaa aagatgataa agatgctact gatgtaataa aagcaacttt
     6001 tccagggggg tctataacag gagcacctaa gattagagca atggaaatta tagacgaatt
     6061 agagccaacg caaagaaatg tatatactgg ttcaataggt tatataggtt ttaatggtga
     6121 tatggatttt aatatagcaa ttagaacaat aatcaaaaat gacaaaaaag tctactttca
     6181 agttggagga ggaatgactt gggattctga cccagatgaa gaatatcaag aaacacttga
     6241 taaagctaaa tccataatga aggctttgag gggatattat gaagaataat gtaggttttg
     6301 atagtagttt aagtaaattt ggaattggtt tgtttgaaac tattagagtt aaaaatggaa
     6361 tagcagtaga tttagatatc catatagaaa gaatgctaag ctcaataaat tgtttggatt
     6421 tagatataaa ctataaaaaa gatttcttga taaatgaaat tgttacatat ataaaaaaag
     6481 aaaatgtcat taataaagca cttagaatta ctgtttttga tgaaggttat aatatatcta
     6541 ttagggatat tccatataat caagagacgt atgagaaggg gtttaaatta gctatatctc
     6601 caatagttag ggggaatagt ttaattcata gacataagac tactaattat tttgaaaaca
     6661 tatataccaa aaatatcgca aataagactg gttataatga tggtatattt ttaaattcag
     6721 aaggtgtaat attagaatgt tctatgagca atattttctt tataaaagga gaaagagtgt
     6781 acactcctag tgatagatta ccgatattaa atggaataat aaaaaaaagg attatagaga
     6841 tttgtgacga attacatata gagttgatag aaaatgaaat taatatatca gaaattagta
     6901 gttttgattt tgtttttgtc acaaatagtc ttatgggcgc tataaaggtt acagaaattg
     6961 acaaaataaa atttaataaa gaaaatgttg tttttgataa aatagtgaag ttattagaat
     7021 aaattaaaat attaacaatt tgttggagac aaaagatgat aggttgtatt tatgaagtat
     7081 acaatgaaga gatgactaaa ataatagaaa accaaaacac tttggctgta tatttagtgg
     7141 gttctagtaa agatgtagat tttactcttt atgatgtagt gataaacgat attgatgtgt
     7201 tcgtttttgt aaaagaaggg gaaaaacaag agagaatact gtttaaaaaa aagggaatag
     7261 aatttgatgt aaattacttt tcaaaagatg gtgtaaaaaa attattatca aatagggaat
     7321 atttctttgt gaaagaaatg aaagatgcta aagtactgtt tgatagggag aatatatcac
     7381 atattataaa agatataagt agagatattt acttagaagg acctagtaaa atgtcattag
     7441 aggaaaagtc ttttataaag caagatattg gagcaaaaat attgaattta aaaaataaag
     7501 aaaaatttga tgtttttgaa tatcattttt tgacgaattt atatttaaaa gatataataa
     7561 ttgggtactt taatataaat gataaatggg taccaaaaga caaaaaattg ctaaaagttt
     7621 taaaaaaaga aaataatgag ctttttgaac tagtgtcaaa agtaagtgaa aattatgact
     7681 atcaaagact tttagatgtt tataattata tttttaaaga aattgagaca aaagaggtta
     7741 taaaactaat cttctagtca caaatcaatg gggggagttt aaatgaataa agttgataaa
     7801 gaaaagatac aacatgcagt aagagaaata ctagaggcaa ttggggaaga cccagataga
     7861 gaaggtctaa tcgaaactcc aaatagagtt gctaggatgt atgaagagat attctctggt
     7921 cttagtgaag aaccaagaga ccatttaaaa gttctttttg cagatgaaaa acatgaagag
     7981 ttagtattgg taaaagatat tccattttat tcatgttgtg agcatcatct agttcctttc
     8041 tttggaaaag ctcatattgc ttatttacca aaaggaggaa gacttacagg gctttctaaa
     8101 cttgctagag taatagatac attggcaaaa agaccacaat tacaagaaag aatcactaaa
     8161 aatgctgcag atataataat ggaagaatta caaccttatg gtgtcttagt agtagtagaa
     8221 gctgaacata tgtgcatgac aatgagaggc gtaaaaaaac caggttcaaa aactgttaca
     8281 tcagcagtga gaggaatatt tgaaaaggac attgcgtcta gagccgaggc tatgagtttg
     8341 attactatga agtagtaatt tattatagat ataatcattt attgagataa aatttaattt
     8401 aagggagtgt cattaattgt gtttgattat ggtaaaagaa cttatataat gggaatatta
     8461 aatgtaactc cagatagttt ttctgatgga ggagatttta ataacttgga catagcaata
     8521 caacatgcaa aagatatggt tgaccaaggt gcagatataa tagacctagg aggagagtct
     8581 acacgcccag gtcatagcta tgtagactca gacgaagagc taagaagagt aatccctgta
     8641 ataaaaaaac ttaaacagga attggatata ccaatatcaa tagacactta taaggcagat
     8701 gttgcagaag aagcattaaa attaggtgtc actatggtaa atgatgtttg gggtcttaga
     8761 aaagataaaa atatggcttc tgtaatagga aaatatgatg cagaagtgtg tataatgcat
     8821 aatcaagatg ggacaaacta tgacaaagat ataatggagt caataaaaga cttttttaag
     8881 gtaagtatag aaatggcaat gagttgtggt gtaaaaaaag aaaaaatagt acttgaccca
     8941 ggtgtaggat ttggcaagga ttttgaacaa aatattgaag tattgagaag attaaatgaa
     9001 ttaaaagatt tagggtatcc tattttgctt gggacatcca gaaaatcagt aataggaaaa
     9061 gtacttccag ttgagcctaa aaaaagatta gaaggtacaa tagctactac tgttcttggt
     9121 ataagagatg gcgtagatat agttagagta catgatgttt atgaaaattt aatggcagct
     9181 agaatgactg atgcaatata tagaaagtag gtataatata atggataaaa tattacttag
     9241 taatctagga ttctatggat atcatggtgt tcttaaagaa gaaaattttc taggtcagaa
     9301 attctttgtc gacatggaac tttatattga ttcaagagaa gcaggtctta gtgacgatat
     9361 aaataaatct gtaagctatg ccgaggttta caatgttgtt aaagatatta ctgaaaataa
     9421 acaatttaat cttttggagg ctttagcgga aaatattgct gaagaagtct tgaataaatt
     9481 tatacttata aatggagtta tggtcagagt tagaaagcct gaggctcctg taaatggtat
     9541 ttatgactat tttggtgttg agataaggag aacaagagat gaataaggct tatttgggaa
     9601 taggtactaa catgggtgat agatttgata atttatcaag agcatgtgaa cttttgaaaa
     9661 atagtgattc tatatataaa gtaaaagaat caagcttata cgaaacaaaa ccatggggat
     9721 atacagaaca agcagatttt ctaaatatgt gtgtagaaat agaaacagag tttgaacctt
     9781 atgagttgtt agaatattgt caagaaatag agagagaact acacagagag agaatagttc
     9841 attggggacc aagaactata gatgtagacg tactattttt taatgatgta gtttccactg
     9901 atgaaagact aacaatacct catccaagga ttcaagatag agctttcgtt ttgataccac
     9961 ttatggattt aaatgaagaa cttataataa atgaaaaaac aataaaagaa catttaaacc
    10021 tcctatctgc tgaagaaaga gaagaagtaa aggagcttgt aggttatgaa agaaaaccta
    10081 tttaataata attcaaagat agagataaat acaaataaca aaaagataac tgtaaaaaaa
    10141 gataaactta taatagctgg accatgtgca atagagagtt atgagcaact tttggagact
    10201 gctaaatttg taaaaagtca aggagcaaat attttaagag gtggagcata caagccaaga
    10261 acatctccaa attcatttca aggattaaaa aaagaaggtc ttgaaatatt aaaagcggtt
    10321 aaagacgaag ttggtatggc tgttattaca gaacttatgg atgtaagaga tatggacgaa
    10381 ctgtatagta taagtgatat aattcaaatt gggtcaagaa atatgcaaaa ctttactctt
    10441 ttaagtgaag tgggaaaaca aaataaacct gttatgttaa aaagaggaat agcttccaca
    10501 attactgaat ggataggtgc ttctgaatat atagcaatag aaggaaatag taatattata
    10561 atgtgtgaga gaggtataag aacgtataat gattatacta gaaatacatt agatttagct
    10621 gcagtaccca ttattcaaaa ggaaacaggt cttcctgttg tggtagaccc aagccatgcc
    10681 actggagtta gatatttggt aaaacctatg tcactggcat cttttgcatg tggagcagat
    10741 ggaataatgg tagaagtaca tcctgaccca gaaaatgctt tgtcagatgg tgctcaatct
    10801 ctttgcttta atgaatttga agatttaatg aaatcaatta ataattatta aaagaggcaa
    10861 gagcctcttt tttattcgta atcgttgcta aggtaaattt ataagttata atttgatagt
    10921 tagattattt aatataaata aagatttaat attgatagaa ttaatataaa aaataaaaat
    10981 tttttgatta ataagaagga tatcaataaa aaaagtcgaa ttgtgtatat tgagtgattg
    11041 acaaatttag aatatagtgt agaatagaaa tgttaacaca aaaaattaca ccagaaggtg
    11101 ataatatgaa tgatataaaa gacattattg aagaaataaa agctagatgc gatatagcaa
    11161 gtataatatc tgaatatatg aatataaaac aatctggagc taattacaaa ggattatgtc
    11221 catttcatgg cgaaaagaca ccttcttttt atataaatac gtcaaaacag atttataagt
    11281 gttttggttg tggtgaaggt ggagacgtaa taaattttgt catgaaaatg gaaaatttgg
    11341 attttatgga tgctgtaaaa attttagcaa ataaatgcgg tatagaaatt aacactaaca
    11401 tgaatgaaga aactaggatt aaaatagaaa aatctaagaa atttcaagat attcatacgg
    11461 aagcagctag attttatctt tcaaacttat tgggaagcaa aaatcttggg tatgaatatt
    11521 taagaataag aggactagat gataaaatta ttaagaaatt tggtttagga ttttctttgg
    11581 attcatggaa ttcacttatg aatactctaa tcagtaaagg atataaaaaa caagacttat
    11641 tggagtgcgg tctaattgca aaaaatagag atggaactaa ttgctatgat aaatttagaa
    11701 atagagttat gtttcctata ttcgattata gaggaaatat aataggattt gggggaagag
    11761 tattagacga ttctttgcca aaatacttaa attctccaga cacgttaatt tttaataaaa
    11821 aacagaactt atacggatta aattttgcta gaaaaaattt agaaagtaaa actatagtct
    11881 tagttgaagg atatatggat ttaatttcgc tttatcagta tggtataaaa aatgtagtag
    11941 caacccttgg aacagccttg actgaacaac aaggcatttt aataaagaga tatgcggaca
    12001 ctgctattat atcttatgat tctgatgaag caggaataaa agcgacttta agagctatag
    12061 atatacttac taaattaggt atcaatgtaa aagtgttaga cttaaaagat gctaaagacc
    12121 cagatgaatt tgtaagaaaa tacggactta gtgattataa aaaagctatg gatgtttcta
    12181 ctcactacat aaaatataaa atagatcatc ttaaaaaaga atttaacatt caaaaagatg
    12241 aagaacgagt gaagttcgca aaagaagctt ctaaaattat aaaacaattg acaagtcctg
    12301 ttgaaattga tttttataca aagtatttaa gcaatcaaat agatattaat gtcgagtcta
    12361 taaagagaga agtttatggt aaaaattaca ataagccata taataacaaa aatcaaaaga
    12421 aaatagaaga aaaagtaatt gaaaaagtcg aagtcagaca agacggaaaa caattagttg
    12481 aagaaactct aataaaaata atgttagaag acaaaaaaat tagagaaata gcgttactta
    12541 aagtagaaga aagtgatttt ttattgaaag aaagtaaaga aattttaaat tacatgatta
    12601 aaaatcaaga attggacaaa ataactattg acaaattgaa aagtttaaac atatccgaag
    12661 aatacttaaa agaattaaat tcaatttctt tgaatagtat aaacttagag aacacaaaag
    12721 aaatagaagg aataataaca aatatcagaa aaaactcatt agaagagcaa attaatagct
    12781 tgttgagaga acaacaagaa ttagaaaata ataatgatat gaaagaggta gatggtagag
    12841 ttatggaaat tgctttaaaa atagttgaga taaataaaat tttaaagagc ttgtagagaa
    12901 aggaggtttg agtgtggaaa ataaatcgaa taaaaaagag ttaaaaaagg ttactgctaa
    12961 gacattaata gaaaaaggaa aaaagcaagg ttctctgaca cttgcagaga taatggaagc
    13021 tttttcggag actgaacttg ataaggatca agtagaaaat ctttatgaga ctttaggaaa
    13081 tttgggaata gaaataacag aaacaaaaaa ctataaagct gatatagatt tttcggttgc
    13141 tgatgacgat ttaagtatag gccacttaga tgaagatgca gaggcaattt cacatgatga
    13201 ctcttctgca atagaaatag aaactgtgga tttatcttta ccaaaaggga taagtataga
    13261 tgaccctgta agaatgtact taaaagaaat aggaaaaatt cctctactta aaccacatga
    13321 agaagtagaa tttgctagaa ggatgcacga aggtgatgag atagcaaaac aaaggttagt
    13381 tgaagcaaac ttaagactag ttgtaagtat agcaaaaaga tatgtaggaa gaggtatgct
    13441 tttcttggat ttaatacaag agggaaattt aggtcttata aaagcagtcg aaaaatttga
    13501 ctatacaaaa ggatataagt ttagtactta tgcaacatgg tggataagac aagctataac
    13561 acgtgctatt gcagaccaag ctagaactat aagaatacca gttcatatgg tagagactat
    13621 aaataagcta ataagagtat caagacaact acttcaagaa cttggaagag atccaaaacc
    13681 agaagaaatt gcaaaagaaa tggaaatgac agaagataaa gtaagagaaa taatgaaaat
    13741 agctcaagac cctgtgtctt tagaaacacc aataggagaa gaagaagata gccatttagg
    13801 tgactttatt ccagatgatg atgctccagc cccagcagag gcagcagcat attctttatt
    13861 aaaagaacaa atagaagatg tacttggttc attaaatgat agagaacaaa aagtattaaa
    13921 gcttagattt ggtcttgagg atggtagagc cagaactctt gaggaagttg gaaaagagtt
    13981 tgatgtaact agagagagaa taagacagat agaagcaaaa gcactaagaa aactaagaca
    14041 tccaagtaga tcaaaaaaac ttagagatta tttagactaa aaagatacat cattgatgtt
    14101 tttgtgataa atgccatgag ataagtgata tctcatggct attttgtagt aaagatttaa
    14161 ttttattttt gtagttattt cagtctaata tctataactt ggattaaatc ctataaataa
    14221 agggaattga aaggagaatt atatttgaaa ttaacagata gattattgaa aatagcatca
    14281 ttagtgtcag atggtaaaaa aatagctgat atagggacgg accacggata cataccagta
    14341 tatttgttaa aagagggaaa agtaccattt gcagtgctag cggatgttaa taaaggacct
    14401 cttgacaatg cacataaaga agtcatacag aacaatcttt tagataaagt tgatttacga
    14461 ttgggttctg gtatagagat acttgaaata ggggaagtag aagaagtgat aattgctggt
    14521 atgggtggaa tcttgataag tgagcttctc gaggctaaaa aagaagtagc acataatgta
    14581 gaaaaattaa tattacagcc aatgcaagca caagaagaac ttagatatta ccttttaaac
    14641 aacggatatg aaattttaga agaagtttta gtgagagaag attttagaat atatgagata
    14701 attgttgcca aatatacagg taaaaatact ataatagaag atgagatata ttatgaggtt
    14761 ggaataaagc ttttagaaaa caaagattct ctttttaatg attttataga aaaaaagata
    14821 aagacatata gttctatagt taataaatta gaagggaaaa atggggaagc tatagataag
    14881 aagagaaagg aaagcgaagt tgcaataaaa aaacttgaaa atttaataaa ataaatagga
    14941 ggtatatatg ctattaaaga gtctaactag aaaaattgaa aaaaagtatc ctctaaactt
    15001 agcagaggat tgggataatg taggtcttat agttggagat tttgatatgg atgtaaaaaa
    15061 ggtattggta tctttagaag ctaatgaaga tgttattgat gaagctatat ccaaaaatat
    15121 agatttaatt gtgacacatc atccttttat atttgggaaa attaataaaa ttaatagtgg
    15181 agatttaaaa ggcagactta tccaaaaact tattaaaaat gatatatcac tttattctat
    15241 gcatacgaat tttgatatag catttgatgg tctaaatgac tattttatgg aaattatgga
    15301 atttggtaat tcaaaggttt tagatataac taaaagcgaa aatttgtata aattagcagt
    15361 atacgttcca cataactatt cagatgaact tagaaaggtt ttaagtaatt caggagcagg
    15421 tcatataggg aattatagtg attgtacgtt ttcaatagaa ggtgaaggac aatttaagcc
    15481 attagaaggc tctaatccgt ttcttggaag tgtaaatgat atagaaactg taaatgaagt
    15541 taaaattgaa actgtagtac cacagaagct tttaggtggg gtaataagtt ctatgttaga
    15601 tgcacatcct tatgaagaag ttgcatatga tttgtacaag ttagaaaata aaggtgagat
    15661 atttggacta ggtagaataa gtaaacttga taaaagtatg acattagaaa gtctttcaag
    15721 gaaaataaaa gagaaattaa atatgaaaca cataagagta gttggaaacc ttagtacaga
    15781 cataacgaaa gtagccgttg taacaggagc tggttcagaa tttgtaaaaa aagctaagag
    15841 acaaggtgca gaagtactaa ttacgggtga tgtaaagtat catgaggcac aagatgcact
    15901 tgatattgga atgtgtatag tggattgtgg tcattttgat acagaagata tattcaaaaa
    15961 tgttatgaag agatttttag atgaatttag tgaaattgaa gtaataaaaa gtaatgtata
    16021 tttgaatcca tttagtacta tttagcagtg tttgataata aaaatactat ataaaaaata
    16081 aatttgagtt taattagggg gataatgatg aaagtagcag taatttttca tagtgtttgt
    16141 gggagtacat atttattagc tagagaatat aaagaagcgc ttgaagaaat gaatatcgag
    16201 gtagatatat ttagagtaag tgacgaagtt gcaaaaacac ttcctcaata ttatttgata
    16261 aattccaaag aatacaaaga cgaatttgaa agtataaatg taattaaatc tggaaaagaa
    16321 atattagatt atgatgctat atttatgggt tcaccaacat attatggaaa tgtatctgga
    16381 ccaatgaaga tgtttatgga tagtttttct gatatttggg taggtactcc tttgagtggt
    16441 aaaatatttg gttgttttgc tacagcaggt tcacaacatg gaggaggaga attagctttg
    16501 caagctatga atatatttgc ccaacatatg ggaatgactc ttttgtcagt tccatgttca
    16561 gtaagaggag gttatccagc gtatggaata cttcatatag caggagataa ttctgatata
    16621 agaccaaatg atgatgccaa gataggtata agagattacc taaaaagact taacatataa
    16681 gaatatcata taaaaatacc taaaagcgac tgtatatact tttaggtatt tttacttaat
    16741 tataaacgtg aagctttacc agaaatatgg tcaatttcta ttttaacaac agttgttaag
    16801 ttcatatcct tctctatgta cttcatgcct tcttcaaaga atcctttaga atattttttt
    16861 attatttcta caagagcatt tttcttttct tcattttcta cagtgcaagc ttttccaaaa
    16921 actatagcac tagaataagt agtactaaat ttatctggaa taacactttc attagcaact
    16981 actaaaaaag aaactttgtt gtttttcgat atattatcta acttatgccc atttcttgca
    17041 caatgaaagt aaatagaatc attaaagtat acataattta cagcaactcc ataaggataa
    17101 ccattttcag atatagtgga aaaagtacca aattcaccat tttttaatac ttcaacagta
    17161 tcttcttttg tcatttctct ttttttcaat ctcatttctt taaacataat atcatccttt
    17221 ctaattttga tgcttaaatt aaattttaga acatttataa agattatgct atgtttttta
    17281 caaattttaa gaaaaaaata tagtttaaga taaaaggtag tgaaagagaa gctatttgga
    17341 ggtttattta tgatattatt gttagagtag tataaacaat actaataaat ttaatagctg
    17401 gtttcttgtt atttaagata gtaagcatgt ctataagaag gggaagttat cgagggtaga
    17461 agttgttatt tataactata ttataaagaa aatattatta tataaaataa taaatagtaa
    17521 agaggtatca agttgaatat aatacttaat acctcttcaa aattaagtta taaaagtgtt
    17581 gtgggaattg tcctatacat ggtaaggaga taataaagaa tgtatcttta aagtctcaac
    17641 taaacagcaa aatttatcca acaaacatac aataaatgct tctttgtatc taggtggctt
    17701 aacagtaagt ggccacatgt gttttaaaat tatgtccgtt tctttttcat ttatttgaaa
    17761 aaataaagtt gcatttttta atgcttctct agggtgagta aatccatgta atccttttct
    17821 atcacctttg taatgccaat cataaagaaa aaagtcgtgt agtaaagctc ctctaataac
    17881 actttgaata tcaacactta agtgcaattt tttacataat agataactat aatatgctac
    17941 tgacaaacta tgttcaagac atgaaacatt gccatgatga ggaaatgttc tcataagttg
    18001 aactttttca gaagaaagta tctcagaagc acattcatta aaatatttga tttcagcttt
    18061 tgacaacatt atgaaaatct ccattcttag taatatataa atactatact tcaacgataa
    18121 tatgaaatat ttcttatcct acaaaatata atacccaata atacaagtaa gatacctttt
    18181 atggtatttt aaagtaagag agattttatt aggctaaaca atgaaaaaat taaaagtaaa
    18241 gctatagcaa tatatagtat ttttccaaga ggattgccaa aatttatagt ccatccaata
    18301 ccaaaccttt tattaaccat taatgatggg tcatttggat tattatacat gatgcctgct
    18361 atccaatact tctcatcttc ttctggagaa taagatgagt tgaattttaa atttggggaa
    18421 ttaatatatg ttgctattaa ataaattata actaaaagcg ttgtaatatt catagcagtt
    18481 gatagttttg tttttattga taaaagagta gtaaaaaact gtgttgtcat tataatcatc
    18541 aatattaaaa acaaataacc aattttattt aaatatttaa tgttttcaac tctactttct
    18601 tcaattctat ttgtatctat cttaatcctt gactttatgg atccaatgga agttatatat
    18661 aataaaacca ttaaacaaaa gtcagtaccg attaatttaa atacattaag aaatgattta
    18721 tctataaaaa catcaggaga cccatttatg ttccaatgag tggcaattga gtctggcaat
    18781 tgattataat taaatgctat aaaaatactc attcccaaag taaatagtat cggtattaaa
    18841 tagattattt taaatttttt tattatttta tttttcttat ttataaaatc catatctact
    18901 attaattttg aatctatatc tatatgacct aatttagaat aaatttctga tttaagcttc
    18961 ttagcttttt tatgtgtatg aatatatagt atactttcat ataataaaaa acctataata
    19021 ccaataaaat aggaaagttc caatttatta aatgtaaaga ccagaataaa aactaatata
    19081 aagtctataa taaaacctaa agataatagt tttttaaatt ctttatctaa ctttataaag
    19141 taattataat ttttatgctc ttggtctata gaaactccat agatgtgttt tttacctaca
    19201 aaatagggca taaaataaaa tgtagcatac atcaaaatta gaactggaaa tattgtaaat
    19261 agacattcaa tcttactcat aatatttcac ctcatacttg tcaaataaac ttgaacttag
    19321 tgacaaaaat tcttctttac taatacctga taaataagat tttgccacca ataattccaa
    19381 gtcaagttta tttttttgac gagttgtatt atctattttc attggtagtt gagctacaat
    19441 agcacctttt cttctatcaa tatttaagaa tccctcttct tttaattcat tgtaggattt
    19501 gttcactgta tgtaaattta caccaatatt ttcagccata cttcttacag atggaagtga
    19561 ttcatttatt ttcaattctc ctgaagctat agccttagtt atttcttctt taatttgtac
    19621 ataaatagat ttatcactat taaaatctaa atttaaaacc ataaaaaacc tccattatgc
    19681 tttataaatg aaaacgattg ttatatacat agtataacat aaaaaataat aacaaacaca
    19741 aaatataagt ttatattttg taaaaattgt aatttaaaaa ataacataat ttttattaaa
    19801 ataatacaga tactatttat agatagaaat aaaaattaga aaggagaaaa aatatgttaa
    19861 catatttaat gaaaagacaa atgcataaaa aagatgatgg aatgagtaaa ccaatgatga
    19921 ttacaggagc tgtaattaca ggtgtaggag catatgctgt atataaaatg gtaaaaagcc
    19981 ataaatctaa ttctcaaatg gtcaatacaa attattatgg aaatagcgat tatgatgatt
    20041 ataaatatga cgaatttgat tatgactgtg attgtagaga cacaaaacaa gattatgaat
    20101 atgaagaatt aaaaaaaaaa gtaaaagaat ttaattcaag aagaataaat tgtgaaaatt
    20161 gtccacatgt ttctcaagaa gaaatgatgc aatatgtaaa tagtgttaaa gatgtaaagg
    20221 aagagattga tttacatgaa gatgctaaaa aagataatat ttacaaagaa gatgaataca
    20281 aaaaatatga agaagaataa gtaatatttt gtataactga acaaatagta atgtcaaatt
    20341 atttaatatc tagttttaat atgtaagttg taacttaact tgaaagaatc agtatcattc
    20401 tttagatagt taagtaacaa cttacatttt atttttataa attttaatag ttctttgtga
    20461 ctattttgta attttagaaa tattttagta tccagggtaa ttgtgtataa taaaacttgt
    20521 atagatagat agtatgaaat taagcagata gtttaaaatt aaaatgaaaa gaaaggaaat
    20581 agttatgtta aagttgttga tagttgaaga tgatagtact atatcttttg gaataaagta
    20641 tgctttagaa caagaaggat ttagtataga tatatcaaag gatttatcta gtggaaaaga
    20701 aatgatttct tctaatgaat atagcatgat actgttggat gttacattgc cagatggaac
    20761 tggttatgag ctttgtcaat atataagagg attctctcag gttcctataa tattcttgac
    20821 tgcttgtgat gaagaagtaa atattgtaat gggtctcgat ataggtggag atgattatat
    20881 aacaaaacct tttagaatta gagaattaat atccagaata aaagctgttt taagaagaaa
    20941 aggtaatact tcagaagaaa ataagaaaat acttaaattt ggagatttaa gtatatatac
    21001 attagaagct agagtatata aaaatgataa agaaatattt ctgacatcag ttgaatataa
    21061 acttctttta attcttatac agaataaaaa tactgtttta agtagaactc aaatattgga
    21121 aaaactttgg gatgtaactt atgattttgt aaacgacaat actctaactg tttatataaa
    21181 aaggcttaga gaaaaaatag gtgatgattt atgtgaacca atctacatag aaactgtaag
    21241 ggggattggt tataaatgga taggaagtga aaatagtgtt tctatataat ccagaagtga
    21301 agaagttttt aagtaagtat gttactttga tgtttattgt tatcataata tctattggat
    21361 ttagcataat caacgtgtcc ttaactaaag agatggttgt aagaaataat caggctataa
    21421 taggaacttt atcaagcaag tatccaaact tggaaagtca gatagtagac ataataactc
    21481 aagggaagtc tatggagaac atagattatg gtcagaaaat attaattaag tataattatg
    21541 ataaatctat taggataaac tctgagccta ttatttctaa attagtttta gagactatca
    21601 aaataaatat aatattagta tgtataatat ttattttgat atttgtgttg gttgttagat
    21661 attttaagtc aatttacaat gatttatcag atatgactaa gtatgtttac tatagttcag
    21721 agggtaagcc atttgatatg aaaaacaaga atcaagaagg gcagataggt cttctaaaaa
    21781 ctgaactctt aaagatgaca acaattctta atgaaaaggt tgaactttta aaaagagaaa
    21841 agatattttt aaacaataca atttccgata tatcacatca attaaaaact cctatgactt
    21901 ctttaataat gttgaatgat ttattatata atgatgtacc atacgaagta aaaatcgatt
    21961 ttttaaataa gataaaaaat caattaaata gaatggattg gttgattaaa agcatgctta
    22021 aattatcaaa ggtagaagca aaagtgataa attttaaaaa agataaggta aaatttagtg
    22081 aactcataca tagagcaatg ctatctatga aaatacctat ggagattaaa aatcaaaaac
    22141 tgactataga gggaaatgat aatgtatctt atattggtga tattgattgg tccgtggaag
    22201 cattagtgaa tataatcaaa aattgtgtag aacatactcc tgaatttgga aatataatta
    22261 taacctataa agaaaatcca ttattttctg aactaataat aaaagatgag ggtgaaggta
    22321 tagataaaaa agatatacca catgtattta aacgattcta tagaggaaga agtagttcaa
    22381 aagaagatag tgtaggaata ggtcttgcaa tgtcaaaatc tataatagaa agccaaaatg
    22441 gcgatatata tgtaaacagc gaaaaaggtc agggtacaga atttcatata atatttcata
    22501 aaatgtatga tagtgactaa tctgtaatat ttaattcact ttatagtaat agccaaaacc
    22561 taatatatga agtatagaat ataaatattg gaggtagtta tggaaatttt aagagtagaa
    22621 aatctaacta aaagttatgg taaaaatgaa acaaaagttg atgctataaa aaatgtaagt
    22681 ttatcagttg aaaaaggaac gtttatagca ataacaggtc caagtggaag tggtaaaagt
    22741 accttattac atttattagg aggagttgac agaccaacta gtggtaaggt ttatataaat
    22801 gatgttgata tatataattt aaaaactaag gatttggcga tatttagaag aagaaatata
    22861 ggtcttatat atcaatttta caacttaatt ccagtcttaa atgtgaagga aaacatattg
    22921 cttccagcag agcttgacaa tagagatatt gatagagaat attttgatga tttaatgaaa
    22981 acattaggtc taattgatag agaaaagcac cttccaaacc aattatcagg aggtcaacaa
    23041 cagagaacct ctataggacg tgcattaatt aatagacctt ctataatatt agcagatgag
    23101 ccaacaggta atttagatag taaaaattca aaggaaatat tagaattgtt aaaattgtca
    23161 gtaaaaaaat acaaccagac actaataatg ataactcatg acaaaaatat ggcacttcaa
    23221 gctgatagaa ttataagcat agaagatgga atcataaaaa gtgatgaggt gatgtagatg
    23281 aatttatata cattactaac cattagatac ttaaaacaaa ataaaagaag aaccattgta
    23341 accataatcg gtataatact ttcaacagct ttaatttgtg gtataggaaa tatatttgaa
    23401 agttttatgg attatcaaat gagagaaact ataaagaatg atggaagttt ccatgttaca
    23461 ttttatgatg taaatagaaa aaatgtggaa tatgtaacga aatcagctga aatagaaaaa
    23521 catgcattta ccaaacaact tggatattct aaattagaaa atagtgaaaa tgctatatta
    23581 agtataaagc aatatgataa aaatgcaata aatggatata aagtttctat aaaagaaggt
    23641 agatttccag ctaaggaagg agaaattgtt ctaagtgaaa gtattattaa tcttatagat
    23701 gataagttaa aaataggaga taagataact ttaaaagtag gggatatgtt tgactcaagc
    23761 aagaaaaaga ttgatagtat gacatttcat gaaggtgatt atatagcaaa tgaaaaagat
    23821 agaactttta aagttgttgg tataattgaa aaacctgggt ttgaacgtta taatggaatt
    23881 gcaacagcta tagcctactt taatcctatg aataattaca atgatacaat taatgtatca
    23941 gtagcagtaa gaaatcctaa agatgtgtat aagataagta ataaaataag taaaaatata
    24001 tattcaaata aagataaaga agcagtttct gatatgataa aatacaatga acatttattg
    24061 aggttacaag gtgctagtaa gtattcaaat ataaataact ctataaagtc tataattata
    24121 gcagtaacta ttttagtaat catatgtaca attgctacag tgtacaattc ctttagtatt
    24181 tctataaatg aacgaaaaaa acagtttggg atattaaatt ctataggtgc tacatctagt
    24241 caaattaaaa gattagtttt tatagaaggt attataatta gtttaattgg tataccaata
    24301 ggactaatat ctggaacagt agctatagac ctattgttta aaattataaa taagtatttt
    24361 actgaatcgg ttgttactaa gatgagtttg cagatagtgt ataatccaat aataactatt
    24421 gttagtataa taatagtttt gcttacaatc tttatatcaa ttctactacc tgcaatatcg
    24481 gcttctaata tttctcacct taatgtgatt aaaaatactg gagagtataa agtgggcaaa
    24541 atcaaaagct caagacttat taaaatgatt tttaaaactg aaggagtact tgcatataaa
    24601 aatattagga gaaatagaaa aaaatttata ataactcttt tttctttaat ggtaagtata
    24661 ataatattta tatcttttag tggctttaca ttacttttac taagaggtga agaaattaga
    24721 aattctcaaa gaaaatatga tttgtattta actgcaaaga acacagctag gtctgtagag
    24781 gatactataa gtgaactaga agatattgat gggataaaaa actttgaatt ggcaacagga
    24841 caatatgtgt ctataagagt aagtgaaaat aaaataaata aatccaagga agatttaatc
    24901 aggaaatatt atcaaaaata taagattgga gatagttatg aatatgattt cagtaataat
    24961 gaattaattt ttccaggaga ttttgcagta aaaaacaaaa taaataactt agtacagggc
    25021 tcttttaata aagagagagc aattgaagaa aatggtgtta tattagttag aaaaagtgca
    25081 tttgaagaaa atgggaagaa aggtgtagta gaacttacca actataaagt aggagataca
    25141 gtaaattgtg aatatcttga tgaaaatgcc ttaagtaaaa aagtaaaggt caagattctt
    25201 gctataactg acgaagaacg tctaggtctt ggttatcaaa atatggggtt acaatttata
    25261 acttatgatg aagttgctaa aaatttaaat ttaaaattaa gtaaaagcct tatatttatt
    25321 gattcaggag gaagcattaa gactaagaag gaaatagaag ctttggcaaa taaaaataat
    25381 tttaactttt atgatgaaag tgcaagcaat gaaagtgaaa aacaagattt aaaggttatt
    25441 aaaatatttg tatatggttt tatagtagtg atatctttag tgagtgttac aaatattcta
    25501 aatacagtaa gtacaagtat aaatctaaga aaaagagaac ttgcagtaat acaatctata
    25561 ggggttactc ctaaaggatt taggaaaatg atatatttag aaagttttat atatggtata
    25621 ctgtcattgt tgtttggaat accaattagt attggtataa ttctaattat gaataaactt
    25681 atttcaggtg taattgaatt ttctccgatt ataccgtgga cagccatagt tatctgtata
    25741 gtaagtgttt ttataataac ttttatagca gggtatatac caatgagtaa attaaataaa
    25801 gaaaatataa
//
LOCUS       DAMKMI010000043        25623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMKMI010000043 DAMKMI010000000
VERSION     DAMKMI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.044"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(273..653)
                     /locus_tag="PC429_001675"
     CDS             complement(273..653)
                     /locus_tag="PC429_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3847187.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
     gene            complement(854..2047)
                     /locus_tag="PC429_001676"
     CDS             complement(854..2047)
                     /locus_tag="PC429_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF3847188.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGIYLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            complement(2110..3366)
                     /locus_tag="PC429_001677"
     CDS             complement(2110..3366)
                     /locus_tag="PC429_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF3847189.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            complement(4162..4896)
                     /locus_tag="PC429_001678"
     CDS             complement(4162..4896)
                     /locus_tag="PC429_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3847190.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            complement(5902..6540)
                     /locus_tag="PC429_001679"
     CDS             complement(5902..6540)
                     /locus_tag="PC429_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF3847191.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            complement(6543..7400)
                     /locus_tag="PC429_001680"
     CDS             complement(6543..7400)
                     /locus_tag="PC429_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847192.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            complement(7402..7776)
                     /locus_tag="PC429_001681"
     CDS             complement(7402..7776)
                     /locus_tag="PC429_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3847193.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            complement(8925..10115)
                     /locus_tag="PC429_001682"
     CDS             complement(8925..10115)
                     /locus_tag="PC429_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF3847194.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            complement(10131..12086)
                     /locus_tag="PC429_001683"
     CDS             complement(10131..12086)
                     /locus_tag="PC429_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF3847195.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            complement(12103..14169)
                     /locus_tag="PC429_001684"
     CDS             complement(12103..14169)
                     /locus_tag="PC429_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF3847196.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            complement(14496..15146)
                     /locus_tag="PC429_001685"
     CDS             complement(14496..15146)
                     /locus_tag="PC429_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3847197.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            complement(16250..16585)
                     /locus_tag="PC429_001686"
                     /pseudo
     CDS             complement(16250..16585)
                     /locus_tag="PC429_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(16683..17363)
                     /locus_tag="PC429_001687"
     CDS             complement(16683..17363)
                     /locus_tag="PC429_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847198.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            complement(17360..18286)
                     /locus_tag="PC429_001688"
     CDS             complement(17360..18286)
                     /locus_tag="PC429_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847199.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            complement(18359..19276)
                     /locus_tag="PC429_001689"
     CDS             complement(18359..19276)
                     /locus_tag="PC429_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3847200.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRAIVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            complement(19276..19965)
                     /locus_tag="PC429_001690"
     CDS             complement(19276..19965)
                     /locus_tag="PC429_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847201.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            complement(20373..20668)
                     /locus_tag="PC429_001691"
                     /pseudo
     CDS             complement(20373..20668)
                     /locus_tag="PC429_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21742..21960)
                     /locus_tag="PC429_001692"
     CDS             complement(21742..21960)
                     /locus_tag="PC429_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847202.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            complement(21961..22368)
                     /locus_tag="PC429_001693"
     CDS             complement(21961..22368)
                     /locus_tag="PC429_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847203.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(22454..22942)
                     /locus_tag="PC429_001694"
     CDS             complement(22454..22942)
                     /locus_tag="PC429_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF3847204.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            complement(23147..24268)
                     /locus_tag="PC429_001695"
     CDS             complement(23147..24268)
                     /locus_tag="PC429_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF3847205.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            complement(24641..24811)
                     /locus_tag="PC429_001696"
     CDS             complement(24641..24811)
                     /locus_tag="PC429_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847206.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            complement(24983..25426)
                     /locus_tag="PC429_001697"
     CDS             complement(24983..25426)
                     /locus_tag="PC429_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3847207.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT         8848 a         4343 c         2691 g         9741 t
ORIGIN      
        1 aatcaaatga acgaatatta tatatatcat tcataaaaac tttatataaa gaatagaaac
       61 aagttctaga ttactataga gatgacatat caaaatattt ttaaaaagca atttaagtat
      121 tagaagtaat tgaagttagt aaatataaat gcgtttactt ttaagtattg cttaagggaa
      181 taaaaaataa aaataaactc tgcaaccatc taaaaatagt tgcagagctt acaaaaccaa
      241 aattatctaa attgtaataa aaataataac atttatacat aagcttcttc ccaatttact
      301 acccagtttt ctaacgactt tatcttttcc tctgttagct cctcttcgtg aaatgatact
      361 aataaattcc ttataaaatt attcttcaaa agattttgca catttcttga tataaactct
      421 aaatatgcct tttctttgat tgcaactgta taatacatac acttgcctgt ttcttgaaca
      481 tataagaatc ttttctttga tagttttgat aaagttttta aagctacttt ttctggtaaa
      541 ttatgttctt ctttcatata atcaataatc tcatatgaat tggtttttat atctaaattc
      601 caaataaatt tcataataac taactctaat tttgataatt tttttaaaca catagttatc
      661 actccgcttc tttatctata taattaaata ttacaataat aatttcacaa aatcaattct
      721 aataatcata acatacctta aaatctgttc ttaacttcta ccttcttaat ctcccaaatg
      781 atttttttat attgtattca atagtataat ccttttccaa aatataactt accagttaaa
      841 ataatcttta tttttactta ttctcgcctt ttacagaagt taataatttt ttataagtta
      901 catcaattcc aaaagctcca agaggtgcag ttataagtat agctagaact gctaacgtca
      961 aaataagttg tccagatgcc atccccattg caagtggtac acctcctatt gccgcctgta
     1021 ctgtagcctt tggaatatag ccaatcatac agaacaatct ttcctttatt gtcaaatttg
     1081 tcttaataag gcagaagaat actcctacca tacggaataa aagaactcca aatattaaaa
     1141 ttacagctcc aattcctgct ttaaatgcat aacttatatc tactgttgcc cctaccaaaa
     1201 caaacaagag aatttcagca ccaacccata gtttcgaata ctttatagac aatcttcttg
     1261 caactacacg atattttatc tgaaggaaaa tcccaatact cataactgct aataatccag
     1321 aaaatggcac aataccctct aaagcagtct ctaaacttat cattaaaaat gagatactta
     1381 aaagcaacac ccctttagca gaatctctca tatgaacctt cttaaaaaat actgctaaat
     1441 agattccaat cactgctcca actattacac ctaatataat tgatactgga atttgtaaaa
     1501 agctaatagc tgatatacta tttccttgtg ccaatcctgt gaatactgta aacatcacaa
     1561 ttacgaaaat atcgtctata gaagttcctg ctaaaagcat ctgtggaata cttttttctg
     1621 taccataacc ttcttccatt aatttaagca ttttaggtac aataattgcg ggagaaactg
     1681 ctcctactac tgcacccatg actgctgctt ccaatataga aacgcctagt aactttggtg
     1741 ctaaaactat cattccgata atttcaaaag tagctggaat aaagcacata agtacagcgg
     1801 gtcttcccac tttcttcaaa tcgtttatat ctaaagataa tccagccctt gttaatataa
     1861 taatcaatgc aatctttcta aggtcagaag aaatatctag tattgaatta tcaatcaaat
     1921 ttaaaacata tggacctaat ataattcccg taagtaacat tcccaataag ttaggtaatt
     1981 ttatcctctt aaaaatacct cccgaggcca ttcctaataa aaaaatcaaa gctaaacttg
     2041 tcaacatatt cctaccctat cctttctcta atattctttc tcttctaatc atacattatg
     2101 ttgttttatt tattgttttg ttgaaatttt ttgtagtgaa taaatagatt tatccatatt
     2161 aaatatatat gaaatagcgc atactacaaa gaaataaggt aaatagctgt acccaaaaac
     2221 ttctgcccct ataaatacag gtgcaaaaaa agtatttgta gcactaccaa agacactagc
     2281 atagccaagc gctgcaacta attcaattgg caaattaaaa aatcctgcta acaatacacc
     2341 aagactagct ccaatggaaa acaaaggtgt aacctctcct ccttgaaaac ctgctgacaa
     2401 agtcagtatt gtaagtataa actttaacag ccagtcaaat gaataaattt ctccaccata
     2461 gaaactattt tgaattaaat ttgtccctaa acctgaatat cttcctttat aaaataaaag
     2521 gaataataca ctaagacata ctccaataat agcaatcctt atcataggat tttttaatct
     2581 attccctatt tttctttttg acaatttcag acaccatgca aatgctccac caaccattcc
     2641 aaaaataatt cctaaaacaa taagcttcca aaatattgac aagtcaaata caactttaga
     2701 agaaagtgca aaagaaaatt tctctaaccc taaagctttt gatactgcac ttgctgtaaa
     2761 agaagcagta aacgcaggaa aaagcgattg atactccagt gaaccagcaa ccaaaacctc
     2821 catagcaaat agtattgctg ctattggtgt ctcaaataat cctgcaaaac cagctgccat
     2881 accagttact agaaaaatac tagaagcatt ttttataggc aaccttttac ctacccaatg
     2941 tgaaaaagtt gcaccaatct gaacagcaac tccttctcgt cctgcactac caccaaataa
     3001 atgggttaac catgtacctg atattataaa tggaaccaat cttaaaggaa taatttcttc
     3061 ttctccatgc cctacctcaa atattagatt cattccttta ctactttttc ctccaaattt
     3121 aaaatagcaa taagcaataa caactcctac tactgctaaa aatggtatca gatacattgg
     3181 atatgtttct cgaatatctg taacttttag aagaaccgtt ccaaatatgg tatctataag
     3241 acctataatt gcaccaactg gtatacctat gagtcctaaa aaaaatagtc caccatatgt
     3301 tttataaacg cttttaattc ttattatcat tttaatccat attaaagttt taaatttttc
     3361 tatcatttta attcctttct atttgcatca taagattttt tgaaagtttt attacatcaa
     3421 ctgtagtcaa tgtaatgact atcataattg ttttttaaat tgataggata taggtacact
     3481 cttaacccat tcttaataaa ataattaaca tataatctac tattttttct attaattaaa
     3541 tatttcttca aatttaaaaa ataaaaattt agtaagtact tcacctccat tttatcgatt
     3601 taaacaacaa aaaaagctgg tggcatctat gatatcatag aagtcatcag ctcattggca
     3661 gtttaggata ttatcatcca agggagaacc tcattcccat aattattata taatttttta
     3721 tttttcatat gtctatttta tgtaaattgt atttttaatg ttataaataa tataacctat
     3781 caattaaaat attaaagtct ctaatattat gagtgttcaa tacttcatat tatttttatg
     3841 atatataatt taaatgattt tctttacttt gcattatgat acatgattta aatatttttc
     3901 ttcactttgt atactaaagt ttatttaaaa tattttcttt cctattatac tatgctgtta
     3961 aatatttttc aactgaagta ttgtagaact ttaaaattac tataaaaaat tatattctaa
     4021 aatacttgta atactacata tcacccttaa cacttaagta taattttaaa gcaaaattct
     4081 tattacttat atttaatttt gattctacat ctttagatat aattttaagt aatttaagaa
     4141 attattgtaa atcatatagt actactcaat aattaggttt ttcaatgttc caattatgtt
     4201 tctttgatat aatccaccat gactaaaaac tatcgtttct atatcataat tacttatatt
     4261 tttaatagca tcttttaact cttgtttgtt gctgtacatc acatcaacag gcttaagttc
     4321 tccattttct acctgtaaca aatctccagc aataagtact ttagactctt ttaaataaag
     4381 acatatgtga cctctagtgt gacctcctgt atgtataact tctatttcct cacctagatt
     4441 caatttttca tggttatcta atgacacatc tactttagta tatgatgatt taaatccaag
     4501 tcccataaca tttagcatac taagcatctt atcatcaatt ttatctacca tttgttccaa
     4561 catataaagt ttaaaaggag tttcttctcc tgttatatag ctgatttcac ttttgtatgc
     4621 atatactttg atattattct tagatttttc tcttagataa tttatgtttc ctatatggtc
     4681 tatatcatga tgagtgatta ttatattttt taacttatca aagctcgtgt tacttttatt
     4741 aagagcatca attattaaat caccttgacc cggaagacct gtgtcaataa gtgttgcatc
     4801 atcttctgtg tagattacag gaatatacat aactttatct tcaccgaata catttgatga
     4861 aatttttaaa acatctaatc cctttgctaa attcataatt tttctcctta taattactta
     4921 attagaggtc aatttaatta aagaaattga gaaaatgtag attttcctct agttatattt
     4981 tattaattaa aaattattaa tacctagtat atcatttaag ttgaaggatt actcgacttg
     5041 atttttttgt tattttatgg tatagtaatt aatatagaag taagtaattg caagctttgc
     5101 aattactttt atttttttct aataactatt ttatttttga taattcgtat aaaattaaaa
     5161 gtaaagtaat atagaaaata tgaatataga aaaactttaa taagaaaata aattatgaat
     5221 aaataaaaga aacgcaatac tgtatataga tgattattta gaaaaataaa atctctttta
     5281 ataaaaataa gcttcaaaat aaacttttag ttcataaagc ttaggagtaa ctaaattgag
     5341 tgcaagtgta tctactgttc aaaataaata gttcataaag cactcttttt tatattaaat
     5401 caattatatt ttcgtcattt cagcaatgaa aaagaagctt atctctattt tgagacagct
     5461 tctttttatt aacgtcctac tttttattag ttttctaaaa ttaaaaagct ttatttatag
     5521 gattccttag atttatattt tgacgatata attttttaaa aacaccttaa aagatggtat
     5581 acaatacaca caatcatact cataattgaa aaataatcaa tggaaaacaa aattactgga
     5641 tattccctat taaaaaatac aaattgattc acaagaaaca tataatctat aatattatga
     5701 tgttactctc ccacaccaag taactttatt ttcaagtaac cttttcatac catctaactt
     5761 cactcaataa aaatggcaat ataaatatat actctatttt ttacttttaa atatctgaca
     5821 agctttatat actattggaa gtaagataca aagtaaaaca gcccaatctg gtaaatgtgg
     5881 catataaaag tcccccttac cttatacaag ctccttatta ctaaaaattt taatagaaat
     5941 aaacattgaa gctaacagta taattaaact taaaaggaac aatacaatat tcactatcat
     6001 ggatggatac tgatatataa atggaatttc aaaattgctg tgagctatag ttaatggtaa
     6061 tacaaatgat gaagccatga ttatcatcac gaaaaagaat tttgttgttt catatcctaa
     6121 tttgtattga ataggcatat aaactccata aataatagaa cttattaata aaacaatcaa
     6181 aatatatgtc aagtcaaaag ttcctatttg tggaataagt aaattttcaa tccagtaaac
     6241 taatgtacaa tagataaata ccattgcaaa taaaacatac ttagattgaa ctaagccacg
     6301 tctaggataa ggagttgaac aaagtaatac agatgctttt gaatattgac tctcctttaa
     6361 taatagatac tgacacagca taaattctga aaatataatt gatagtacaa atcccaatat
     6421 ttgtgcaaat ggaggtaaac gccataatat aaataatggg attcctattg caataaataa
     6481 tatcaacaat acatattttt ttacaatcag aatatctttt ttaactaaat gaatcaacat
     6541 aattactctc tcctttcaat atatgcaagc ataatatctt caattgtagg acgctctatt
     6601 aaaatatctg gcatattttt ttgcacttca ttaatttttg aggtaatccc tataaaacca
     6661 taacctgtaa tttctaaatt taaaaataat tttttggttt catcatttag aaattgaata
     6721 tcccctttta cttttctatg ttcgtccaat agagtatctt tttcacgctc taatataata
     6781 cgtccattgt caatcataac aatcatatct gctattttgt ctaaatcaga tgttatatga
     6841 gtggaaaaga aaacaccacg accacatttc ttcatatatc ctgttagtat gtcaagtatc
     6901 tgactacgga ttagtgggtc taaaccactt gtaggctcat ccataattaa aaactcagct
     6961 ttatgcgata atgctaatgc taaagcaaat ttcattttca ttccttttga aagtgtatca
     7021 atcttttgtt ttgggttaag attaaaacgc ttcatatttt gttgataatc ttcttcacac
     7081 caattatcat atgaaggtgc aataatactt ttcatttctg ccattgacaa ctcctcatag
     7141 aaatatgaat catcaaagac aactccaatt cgatttttta attccttctc atttccatcg
     7201 aaatctttac caaagatttt tatttttcca ctatcacgct ttacgacatc caatatcgaa
     7261 cggattgttg ttgtttttcc tgctccatta acacctataa atccggttat acaatcttct
     7321 ggtaatgcaa agcttatatt agataatgaa aaatcggtat aagtcttatt taagttataa
     7381 acttctaaaa tattttccat attaatcctc ctcatataga atatccatca tttcttgtaa
     7441 ctctatctgt gtaattccta gtgcttttgc atctttaatc atatctgcca ttttttgttc
     7501 aactatacgt ctctttgagt ctcttagtat ttctaaattt ccagttgaaa taaatgttcc
     7561 tattccaact tggcgtgtaa gaaagccttc attttcaagt tcttcataca ctcgtctaat
     7621 agtcaaaata ctaactttta aatcattagc aaaatttctt atggatggca ttgcatcccc
     7681 ttctttcagt tcttcagtaa atattgcttc tttaatttgc tctttaattt gttggtataa
     7741 tggcttatct gatgtgttag aaatgagcac tttcattttt tatcaccccc tttttatcac
     7801 tttcagtgtg atgttatcat atacacacta tacacttaga gcactttttt gtcaatataa
     7861 aagatgtaaa atattactat gacaaaataa atatgaatga aatattagag attgttaata
     7921 gccataaaca ctttttaaaa ctacataaga taggatgtat taaatattta ttaaaagtaa
     7981 gaattgaata tataaaagaa aaagtaatcc tgtatgaatg atttgtttaa tgataaatta
     8041 agttataaac tatttataaa aaacataaag gaataaatcc atttctaatt acaaacatac
     8101 taaaaaattt agataataat aaataaatag taataaatgg gatgaattta taagaatttg
     8161 gatagaggaa agagttattc aagagtgtag actagaggta aactctatat taaaaaaatt
     8221 taaattggga tattattatg tatggaatat ttgcagaagt aaccttgcta taagtataga
     8281 agattttttt gattacaaaa gataatactc taaaatgtga ggatttcaac attcaatact
     8341 gtgctttaaa taatatcact ccaaaatacc atccaccttt tactataatt gaatacccta
     8401 attattttaa aaaagaaaac attagaaacg gaaaaccttg tatatataaa ataaaaaata
     8461 ctaacttcta aaacacgaat actctttgat aaataaaaca aaaaataatt ttaataatta
     8521 aaattggcta tctcaaacta gagataagtc tctttctcac ttttaaaacg ttgaaaatat
     8581 attcgatttc gtacaaaaaa gagtgcctta gaactaaaaa gttcactttg tggcactccc
     8641 ttttttctcc tctttttaat tttttataaa aattcttcaa aaatattatt aaatacccta
     8701 tattatacat aaatcaaatt aaagttgcat tttagcaaat aaaaacaaat tataactatt
     8761 agcatagcaa ataaaaaatc tgctatctta taaaatcaaa attataatta taaaaagaaa
     8821 atattcttat aacaaattaa ctaatataaa attattttat aaattaatac tactttttag
     8881 cttctcctat aaattaatac cactttttta actatgaaac ctatctacaa cattggtacc
     8941 ataagaccag ctaccacaat agaaatcata caagccatac caccaactac cagcttaggc
     9001 ataatcttag acatcaaata tgctctttct tcttccgttt ctccaacagc attacacact
     9061 tcatttgaaa ttaaatatgt agttgggaat cccaacatct gacaaaaccc tactccaaaa
     9121 gcaagaggtt tagaaccgat aattttccaa cctggtaaca ccttcatcat aaggaatata
     9181 ccaatgatag aagctgcaaa catacccaca attggtacaa aaagactaat taaatctgtc
     9241 aaagaaactt ttgctaaaga aggaataaca gctgcaaaca caaccatgtt gataaatccc
     9301 ttgatttttc ctttttccaa aatgtctttt ggcacaatac caatacgtgt tacaataaca
     9361 ccaacaacaa gacatacaat agaataattt acatgaataa tctctgaaag ttcataccct
     9421 agataagaga ataatgctat aaaaaatata caaacatttg tagaataata tttatcgtat
     9481 ttttcacaga aagcttgttt tacttttgtg cctgtattct ctgcttcttt cttatcagta
     9541 tcaacatttg ctaaatcaat gccttttgat tttgcttcat gatattctac taaaagacgg
     9601 tttgcctctt gtaacgctgc tcttgaagct attggtgtac caataaactt ctgaattgca
     9661 aatacaactg ttgctgtagc tgctgcaagt gtcagtcctt tttctagtgc tgcttgtgtc
     9721 ataatagtag tagcaggtaa agctccattt ataactggta tgcttgtcaa tactgttgat
     9781 tttccaaaca aaggaataca taatgagaca cagactataa ctgctacaat tccaagccat
     9841 gctgtcaata ctgttctcca ttcgtctata aattgtttaa cattcaacat tgttcccata
     9901 ctaaacataa tcattggaac actccaacta gctgctgcgg tcatacctgc tttttcaata
     9961 atatcagctg gtattgcttt tgtgacaaat agaatcaaaa ataaaagtag tgtaacaaat
    10021 acagctgata cttttgcttt tgttttgctt gcaattaaat ccccaatagc aaataaaact
    10081 aatacgacac taaaccataa taacatatca ctcatagaat aaccccctta ttataaatat
    10141 ctttcaaacc aatctacaat agcatttgct cttgcaattc tattagatgg tcttccattt
    10201 ctagacaatc catgtgtatc tccatgaaac aaatacattt ctgtatctac accaagctgt
    10261 tttaatgctg taaacatttg gaaagtatcc cccataaaac atctataatc cctatcacta
    10321 tgtaagaaca atgttggtgt cttaacattt ttcacatatt taataggtga atgattccaa
    10381 agaacctcct catgattcca aggcgtctta tctccacaag tctgcttcat aattctatcc
    10441 ccaaagtcaa taattaaaga agctgtaatc aagttactaa tacctctttg agaaactgct
    10501 gctttgaaac ggtctgtatg accaattatc cagtttgtca tataaccacc gtaacttccc
    10561 cctgcaaccc ctaagcggtc tgcatcaatc ccaggatatt tttcaataca agtatctaca
    10621 aattccataa gatgctcata gtcaacatac ccccatcttt ctaccatttc taagaatttt
    10681 tcaccataac cgtcaccacc atgagggtta caataaatca catacattcc ctttgatgtt
    10741 acacactgct tcaagtgtgt aaacatatag ccatatgccc atttaggccc accatgaatg
    10801 aacaatacag caggatattt ttcgccttct ttagcatcta taggtggtat aacaaaacca
    10861 tcaacttctt ctttattttt tgctacaaac gtcaattttt caggtaatga aaccttatga
    10921 gtatctaaat atggtttaga aaaagcagtc atttttttca ttgtacctgt agtcatatca
    10981 atgctatata tttctgctaa ctcataatct ttcaacatta ctgtgtacat ggtgttgtcg
    11041 taaatatcaa aagatgtaaa agcatctact tttgtgatga tacgtatatt acctgtcttt
    11101 gtatcaaact cattaaattc tgtcatagtt ctgcaaattc ttgcaccaat aaatttgtct
    11161 cctataactt tattactacg tccaccacca tatactgcat ctacatccag caatgtagca
    11221 tacataccat ctggactagc aatcatattt atttcattag tactcaaatc aagtataaaa
    11281 atatcatgat tctgtgtgat agaacggtct aatgttgaag cttgcaacat tactttatct
    11341 ttgccaatat attttgccaa acttatttgg tactttcctt gtggtacaag ttctttggtt
    11401 tcaccactgt ctatatgata ctcccaaagt ccagaaaaag gagttgcaca atcttcataa
    11461 atctgaccag tatataaaat tctgttttta tcagaagaaa cgtctacacc tgctacttca
    11521 caaaattttg gtgtaatggc ttttgttata ttttcacctt cttgatataa ttctaatgtt
    11581 acacgtcttc tttgagaaaa attttctcca tctcttataa aaggtttatc tgtaaatgtc
    11641 caataatcta caccctctac tgccctatct ggttctgatt ttttagaatc cgttggattt
    11701 tttgttgtca gtaaccacaa tttatttcca agtgatacag gtacgctaca agcttttgga
    11761 atagaaaatg ctttttttgc ttctcctcct tctatagaaa tagtgtaaaa atctgttgta
    11821 cctgattttg gcttatttct tttagatgta aaaaggagtt ctgtattaga aatccaacta
    11881 aatgatgtta catcttttgt ttgagtcata tgtatttgtt tttctgtttt gatgtccatc
    11941 acatataagt catgttcata gcagtctttt tcctcgttga tatttgatat aatataagca
    12001 atatgctttc catcaggtga acaagaaata tcatgaggga atatatattc aaaaatatct
    12061 gaattttcaa tctttatttt ttccattttt atattctcct ttctactgtc ctatgttatc
    12121 tttttttata tatactgaaa accaatctag catttcaact aatctttgta gcttttgttt
    12181 tggtctacca gaacgagaca gttcatggtt ttctccttca aatagacaaa gttttgtagg
    12241 acacccatga attttcaatg ctgaaaacat ctggaatgct tcttgcatat agcatctata
    12301 gtctttttca ccatgtaaaa ataaagtcgg tgtaacacat ttatcagcat aagcaagagg
    12361 tgactgtttc cattgaagct cgtggttttc catatgagaa gcacctgttt gtcctttgca
    12421 gaaataataa ccaatgtctg ttgtaccctc catgccactc cagtttgcaa tagaacgctg
    12481 agcacaagca acagcaaaac ggtttgtatg accaattatc caattcgtca taaatccacc
    12541 ataacttcca ccttcaacag ccaattttgt tttatcaatg tctgtatatt tttcaagtac
    12601 agtatctgta aattccataa aatctatata atcaatatct ccaaactgtt tcctaatatc
    12661 tgcaaaagca ttgccacgac cttcagaccc ccttgggtta cagaagaata caaagtaacc
    12721 tgcacttgta aatacctgta tctcatgcat aaaatatggt ccatatgtca atcttggtcc
    12781 tccatgaatc gccaatacag ctggatattt tttccccacc tcataatcta ctggtttata
    12841 tacgtatcct tgtatttccc atttagaact tccttttgac tctatgtatt ctggcacaga
    12901 aattttatat tcctctaaaa tatggtcatt taaatgtgtt ttcttacaca atgttccatt
    12961 ctctactgta taaacttctg gtaatccatt tcctaaacaa cccacacata caatattgcc
    13021 atcttttaca tctataaaat caactgaacc tgtctgtgtt actttttcct gttttcctgt
    13081 tggaatatgt attctttcaa taatgcagtc catatctact gtagaagtgt aataaatata
    13141 ttctccatct acctttgttg tctgaccagc acccatttta acatcagatg ttactgtatt
    13201 tccaatacaa tgatgttgat gccctgaaag aagtttcatt tctttcgtat caatatcaat
    13261 agtataaaaa tctccatttt ctccatttcc ataagataaa gcatcagtta ggtttagtat
    13321 cacttgattt tgatataatt caaaaccttt tataatatac ttatcttttt ctaatataca
    13381 gacatttgtg tttgtgtttg tatcaaatac atagacccca tttttcaaac cttttacatc
    13441 tttaaactct tgccctatat ataagatttt tccatcactt attttcatac ctacaacatt
    13501 aaacataggt gctgtaattg ccttcaactc atttatatta ctgtcgtata catataggcg
    13561 ttttcttttt ctgctaatat atccctcccc attgacagca taaggaattt ctttgaaatg
    13621 ttcataatgt cttttgttat aagcatttat ttttttctgt tgttcttctt ctggcaatcc
    13681 ctctacaata ggtcggtcat tgtcataaga atcaataact gcatacaaac cttttcctat
    13741 aggattcaat ttaccacctt tgatagggag tctaaaaagt tcttctgctt ctccaccatt
    13801 taaagataaa gcatagtaac tcatgcattg cttaccgttt ttgacagctt cttttacttt
    13861 ttcatttcgc aaagcaggga acaagatagt attttcatct tcccaggcat atgctccaac
    13921 agagtttata gacgttattt gttttaattc gccagtttta ttgtttaata tataaacttg
    13981 gcttgtgtaa ccattctctt tgatatcagc tttttttaca ataaatgctg tatatcctcc
    14041 atctggtgat atttggcaat cacttataaa ttgatattcc aaaaatgttt cactcgtaaa
    14101 tagctttttc atagtatttt ctatcctttc tttagtaatt tttttcaaac tctctaccac
    14161 ctctttcatt ttttatttta aatttaaaat aaaaaatttt aaatttaaaa taaaaaattt
    14221 tataaaggtt atttttttag gaagccttct ggcttccttg taatatttgt aatttaaagt
    14281 atatttaatc catgcttaat tcatgcttaa ttcatactta atctatactt aatctatact
    14341 taatctatat ttaattcata cttagactta tttacactat atttagatta tatttattag
    14401 attatattta gattatgttt aaactataat tagattatac ttagactaca tttagcaact
    14461 taatagataa gtatatatag taacttcaaa acttattatt catcttcaca gaatctttta
    14521 atagttcctt gaatatgatt tcttagttta tctactgctt tttttgtatc tctgtcttca
    14581 atgaatttta atatttcaaa atggtcttgt actgttcctt cataattacg attttttaat
    14641 ggtcgaatta aatagttcac tactataaat cttccttgta taccaacata tacatcattt
    14701 aaaatctcat tatttgcccc ttctacaatg gctttatgta attccaaatc tgctagtgca
    14761 agttctacag atttttcttg acgatagtac tcctcgcatt tatcagagta tctttttatt
    14821 acatctagct tttgttgaaa aacctcttcc tcttcttgca tcattttttc taaagcatat
    14881 gtttcaatca gtgttctcgt ttcacaaact tgttttacca tatcatattc atactctggt
    14941 acgtacactt taccatttgt cataatagta agcactccat ctttatttag catttttact
    15001 gcttgtacaa ctggtgttct gcttacttca aatttttcac aaaggtcatt tagtaatatt
    15061 ttttgaccta cagagtattc accacgaata agactttcaa taatcttatg atataaaatt
    15121 tcatctaaat ttgactttac tttcactact ctcaccttat ttctattctt ttgccatatt
    15181 atattgaatt actggacttt aattactttt agtaatattt ttaaagtaga aattcttatt
    15241 tgatagtact tttgtactac aatttaaatt attgtactgt aaatcatgct cttatactat
    15301 agattaattc attgtattgc aaatcaagtt attgtactat agattaaatc gttgtacttc
    15361 aaattaaact attatactat agattaaacc attgtacttc aaattaaact attatactat
    15421 agattaaatc gttgtacttc aaattaaact attatactat agattaattc attgtacttc
    15481 aaattaaact attataccat agattaattc attgtattgc aaatcaagtt attgtactat
    15541 agattaaatc gttatacttc aaatcaagtt attgtactat agattaaatc gttgtacttc
    15601 aaattaaact attatactat agattaaatc gttgtacttc aaatcatgct agtgtactat
    15661 agattaaatc gttgtacttc aaatcatgct agtatactat agattaaatc gttgtacttt
    15721 aaattaaact attatactat agattaattc attgttctgc aaatcatgct agtgtactac
    15781 aaattaaact ataaatgttt actatctctt aataatttga ataatatgtt acaaaaagta
    15841 tagcaccttt ttattttttt cgcaataaat aaaaaattta aaatttttta ttttttattt
    15901 tcttatatta tcaatttgaa atatgaaaga ttaagactaa tagaattgat tcagttaatt
    15961 gaaaaaaata ggtacctttt atgaaaattc taatgataag catttttctg atgtaatggt
    16021 aaattataaa tgataaaatt aaaaatcact tttagtttta tgaactgtcc ccatattaag
    16081 aaacaactaa gatacataaa aatagttata aagagatagt taaactatat ccactatttt
    16141 atatagttaa aatagtgggt atagtccaag ctaagaataa ctaagaaatt agcgttaaat
    16201 agcatagata aaaatatatt tatataaaaa ctttttttgc tgcaaacaat tatttccata
    16261 acattttata tacttctcta aaatcttcta aacttgataa atattcaatt aaagggacat
    16321 aattacctaa actacaagaa tttaaggagt ttctatacaa ttcctcacta tcccaagata
    16381 caatttttat aggaagatta ttttctaaca tctgttttag cataacaaat ctacctatgc
    16441 gaccattacc atcttgaaat ggatgtatca attcaaatct ataatgaaat tcagctataa
    16501 ctttaataga tacttcactc tgactatagt accattcaat taattcatca agctttggct
    16561 caacttcaaa tggttgtgca atattattaa aactctaata atattaataa tagctgatat
    16621 aatagcaaat cattattatc ccaagaagat acaattaata actaccctcc tatccacagt
    16681 atctagtcaa tttcagtaac tttatgattt agtatataat atataccaat cacagttata
    16741 ccaactataa taaaaacaga aatcaaactg tcttgagttg tatttgtacc agctaaaata
    16801 aattgtctta aattgataaa aataattgaa ctgacaaggg taacaggtat tgattttctt
    16861 atcattccaa caacgaatgt ccatatacct aatgagcaaa atacaataat acttttaatt
    16921 gctcctggta tccaataaga taaaattgaa atatgaaaat ctccaataac taaattaaaa
    16981 tacttatcca acacaacaac gaatgtaaaa caacagatat atccaactat cattgatgat
    17041 atcataagag tcgtaatcaa aagtaacttt gataaaataa gttttttacg gtcaagtgga
    17101 taagagaata aaatcaagat tgttttattt gtatattcat ttattatata tgccacattc
    17161 aacacagcaa ataatataat atagtaaaaa cagaaaaata aactgattgt cctaaatgta
    17221 acatcataat catatattga agtagattta tcattcaatc cagtaaagac aaaaaacata
    17281 cataagaaaa tccccagcat tgaaaataaa aaatatcttt ttagcccaac tttttttagc
    17341 tctagtttca ttaaatgtat catagtgttt ctttacctcc tgtacctgta gtttggaaga
    17401 agtaatcttc taaagaactt tgtttacgtc caatgctatt tataccaatt tgattttgaa
    17461 taaatatttt ggatatttct tttacatctt ttcgtaaatc atatatttca atacaacttt
    17521 ccgaagtaat tttaaagtct tttattccaa attctttttc taacaagtat ccagcaagct
    17581 ccacattatc tacatctact tcaacataat ctgtttgata tttgtgtata tctgacatag
    17641 aaatttcttt aataagttta ccatcttgaa taattccaat agtgtccgca atttgttcta
    17701 cctctgaaag aatatgacta gaaataaata tagttattcc ataatctgta tttaacttac
    17761 ggaataattc tcgcatttct cttatcccct ctgggtcgag tgcatttata ggttcatcaa
    17821 gaatcaataa ttctggttta gtaagaattg ctctagctat tgcaagacgt tgtttcatcc
    17881 ctaaagaata ttttgaaact ttcttgtttg attgctttga caatccgaca cattccaata
    17941 cctctgatat atgttctttg ttataataac ccatatattc acagtgtaaa tcaagattct
    18001 gtttagcagt caaattctca tataaatacg gattttcaat aatagtacca atctttttta
    18061 atatttcaaa atcagtttct gccactttct taccgaacat aactatttct ccagaatttg
    18121 gattaacaag atttagcaac attttcatta ctgtactttt acctgccccg tttggtccaa
    18181 caaatccata aatttctccc tttttcacat gaatattaac agcattaaca acagctttat
    18241 ctttatatat tttctttaat tgatgggtct ctatgatata gttcatcata ctccctcctt
    18301 tctaaaaaca gtatacaaaa taaaattgtc ttttctctct tttaaatcta acttttttct
    18361 aacttttcaa ttttaccgta aaaactgttt ttacattagg tctactattt acataaattg
    18421 aacctcccat agagtttgca agcccttgag aaatagccaa acctaaacca ttgccttttt
    18481 ttctgtcagc agtataagct cttgtgaaaa tatgctcttt ttcagtttca gaaatacctg
    18541 aacctttatc ctcaatatct ataaaaacat gtttttcctt ttcatatagt gaaatcccta
    18601 aaaatctacc atctgaacca tattttatag cattatcaat taaatttttg agtatacgtt
    18661 gtaatgcttc tgtgtcaaca tttgcatata taggtctatc ttcaatatta acttcaacat
    18721 taaagttttc tttatctagc aaatcataaa actctaaaat aatctcatga cataattgtg
    18781 ttagattgga tttttgaaga tttaatatca tatctccaga ttggattttt gccatattaa
    18841 acaggttatt tattgaatta acaagctcat ttgaatttgt ttgcattttt tccacaattg
    18901 ctctcattga agaagataat tcttcttttt gactttgaag atatagcatt tcaatataac
    18961 cctttaatac tgtgatggga gttcgtaaat cgtgtgatat gtttgtaaat atttgcaata
    19021 tcgctttttc acttctatta taattaattt gcctgctgta aaacttttca acaagtttat
    19081 ttatatctac tgctgtttct tttacaatcg aaatatcaga tggtactaaa atataattaa
    19141 tttcatcatc atttgttatg tcacgaagtc ttgaattgat atacttataa tcactttcca
    19201 ttttctttga tttatttcgt tcttttaagt agagagcaaa tagtacaatc gaaattacaa
    19261 ctaaaaataa ctccattatt cttcctccat tttgtagcca attccccaaa gagttttaat
    19321 ataattattt ttttctatac catcagacaa tttatttctt aacctgttaa tatgagtatt
    19381 tagcacattt tcatttccat aataaggttc tttccaaata gcgttgtata attgttcctt
    19441 tgaaaaagca cgtccttgat tagtcgctag taatttaaca atttcaaatt ctgtatgtgt
    19501 taagtttaag tcaattccat tctttgaaac tacatatttt gatatgttta taatcaaatc
    19561 cttataccga ataaccatat cttgagagtt tcttgaactg ctatacactg ttgcacgtct
    19621 aatattagct ttaattcttg caagcaattc tacgattgaa aaaggctttg taatataatc
    19681 atctgcacct agattaagcc ctagcatttt atctgtgtca ctatctttcg cagtagtaat
    19741 aataacagga acaacactca cacttcgtat atattttaat acttcaaaac cactagactt
    19801 tggtaacatc aagtccagta aaataatatc aaatgaataa tttttaaata tttcaatagc
    19861 tgtatttcca tcaatggcag ttttaacaga gtaattctca ctaatcagaa aatcgtatac
    19921 catttctcga atattcttat catcttcaac caataagatg tccattatta ttcaactcct
    19981 tttcgtcagt atacttcaaa tgatatattt attaaaaagg gttgtcaata aataactgct
    20041 tatgatattg tatctgcaat ttggtgatac tcttctaagt taaagttctt ttcaataagc
    20101 tttattgaaa aaagaattaa aatatagtat actctcttat ctttatctaa aatatacata
    20161 attttctttt attaacatct acaattaact attttatatt ataaaacacc ttttaacttt
    20221 tgctaaaaaa tattttatac ataaaaaaga aaccacattc tagttttata cagaacatag
    20281 tttctttaaa aataatcaaa tttggatttt aaataattaa aaacaaactt ttatttgttt
    20341 ttaattttcc aattttatag tagataattt agttaattag ttcttaaatg tttatcttct
    20401 ttagtaaatt tttttcttat taagaaatat gcaactatta caacaagttc tattgctaaa
    20461 aatacaccat aagtagctat agaatctatg tatctatgcc atttctaaag aaaattaaag
    20521 caattcctgc tgctataata cctacagcat tttaattttg accaaaacca taaaaagaag
    20581 ggaaatttcc ttttatttca atgaatgtca agattaatcc aatagctata aatagtactc
    20641 ctacaacttc cataatcatg ctaggcattt tgacccctcc ttattaataa ttaactacta
    20701 ctataaaaat atttattatt tttataatta tattatatat gcgattacat gtttttggaa
    20761 gtatatttgg aagtaatttc atatttattt aacttaattt ttttgtgata aaataattct
    20821 aattttcata tagatattat tataaatttt ctagtttatg gagttattca ttctataatt
    20881 cacaaatatg tattttttaa tctattaaac tattcatttt ctggcttata agcatggact
    20941 tcttatctat aagcttggtt tttcatctaa taagttattt attctttaat ctatgagctt
    21001 gatttttcat ctataagtca tttatttttt aatctataag tttagttctt catctataaa
    21061 tcatttattt tttaatctat aagtttggtt tttcatctat aaataagtca tttatttttt
    21121 aatctataag tttagttctt catctataaa tcattcattt tttaatctat aagcttggtt
    21181 tttacctata attaattcat tctctaatct ataagcttgg tttttattta taagtcattt
    21241 attctctaat ctataagctt ggtttttatt tataagtcat ttattctcta atctataagc
    21301 ttacttctta atctataagt catttattct ctaatctata agcttacttc ttaatctata
    21361 aatcattcat tttttaatct attagcttgt tttttaccta taattaattc attctctaat
    21421 ctataagctt tctttttcat ctataaatca ttcattcttt aatctatgag cttggttttt
    21481 catctataag tcatttattc tctaatctat aagctttctt tttcatctat aaatcattca
    21541 ttctttaatc cattagcttg gtttttcatc tataagtcat ttattctcta atctataagc
    21601 ttacttctta atctataaat cattcactct ttaatccatt agcttggttt ttcatctata
    21661 agtcatttat tctctaatct ataagcttgg ttttttacct ataattaatt cattctctaa
    21721 tgcataagta atacatgaaa tttaaatatt attttcttca ataaattcaa acaactcttc
    21781 tacagttgta tcaaatacct tagcaatatc cattgccagt tttaaagaag gattgtagcg
    21841 accattttct aaatgtacaa tagtttcacg tcttactcct actgactttg caagttcacc
    21901 ttgtgtcata aggttctttt ctctatagga tttaattttt gtctttagta atactgacac
    21961 ctaattgtat atcccctttc tgtcaaaata tataaataga attaacttca acaaggattg
    22021 aataaacaaa atgactaata aagacattcc tatatttaaa tttgacacat ttatgtccaa
    22081 agttacaaga aatatagata ctatagctac agaaaaaaac aatactttta ttgcaatatc
    22141 attaactctt gatagtatac attttgatgc ctcatcaatt aaatcatcat actttttatt
    22201 atagacccac tgagctccca tagctaatac ccaaataatt ataggaagat attcaatact
    22261 acttccttta aaaaaatact tatttactaa tgtagctcca atacaataag aaattcccaa
    22321 tatttctgaa ataagtgttg atttaatacc agtttgttgt ttagacatat aaaatccccc
    22381 ttaaaaagtt atgtatttat aacacctctt gttataaata tataactttt ttgaataaat
    22441 ttcaataaaa atattatttt gcaacaaatt cttttattaa ttttacatta gatagaggca
    22501 ctttagatgt cactccttgc aatgtcaaat cttctatatt aacttcattt aattttgaac
    22561 acagttctaa gtcaaatatt ctttcccaac ttaccttctt ttcaatttgt tcatcttcat
    22621 aaagctctac agtaatatcc attaaaggta aatgccataa atcaaattct gataataaca
    22681 cattgtcttc acttatttct atttcaagta aaacaccttt ttttcctttt tctaaataac
    22741 cactacatct taaatcaggt ctcttcgtcc aaacccatat catactatca tcaaattcat
    22801 atattctatt tgtcatttga ctatacatcc atttgtatgg aatatcaaag tcttctaata
    22861 taaaagaata atcatttgat agatatttat tgtttttatt gttattccaa aattctaaag
    22921 tctgtatagt ataaaatttc atatataaat caaccccttt gtttttctat aagaaggaga
    22981 tggttcaaac ttttattttg aattatctcc tttatttata cacctctttg tacctattat
    23041 ttatatatct ctttatatct accatattta aataacaaag ttttatatta atttaaattt
    23101 aaaagaggaa gctttgtatt tatctagtgc catctcaatc atagtattag ttttgattat
    23161 agactttttc aacctctgat ttattcaaat catctataat atcgcatatt tcactcaaat
    23221 tgtatccttt attttttaat tttatgatga tattaaattt ctcttctctt ttcccttttg
    23281 caataccttt ttcgatacct ttttcgatac ctttttcaat accttcttga aaaatttctt
    23341 ctttaaaatt tctactttta ttcataatat acgcacctct ctttaaattt aataaatagt
    23401 cctcattact acctaattct tttaatctat caagtgctct tttaataaca taatccgttt
    23461 tagatagtat tctaatatct atatgagaag gattatcaat aagtttaccc catattagaa
    23521 ctctgtttgc tatacaactg gatttacgaa tttttggaag ttctataaaa tataatgcag
    23581 ttttactaga aaaaatatct ggatgcccgt ccattgttaa tctcaggcaa tttacataat
    23641 cttctatgtg attatatttt acatgattta gtatatgtat tccaacagtt tttccaattt
    23701 ctttatattc tttaccttct tctaactttc taccatgtac acgactaaga taatattcag
    23761 ctctttccaa ataatcctct tcaagagctc tttgcatctc aatatttata tggttacgtt
    23821 cttcatcttc agccaaaata tcaagtctag gtcttttctc accaatgtaa ttaattgttg
    23881 ggtcggtttc aaccttaatt attcttttta tttgagtgaa ctgtttgata ttcctattag
    23941 catatggagc atctttgagg atatcattta taaaatttgc aagtaaatcg taatcattat
    24001 ttaaagtaaa caatgattta aatacctcat catactcagg cttagcatac ccatcttcag
    24061 ttacaacttc tccaacattt tcactagtca atatttcttt tggaattaaa tccttgattt
    24121 tatctaattt ttcattagaa aaatcacctc tactaagtat gtcatttaat ttttgggtcc
    24181 attctctgtc cttttcttca attatagttc gtttatactc ttttgatgtt ttaataatct
    24241 tagatttttc ttctaatttt tctttcatat caacacctct tttttattat tacccatttc
    24301 ttcataccaa agtctgaact tctaagtaaa atttgcatta ttgaaataag aaccttataa
    24361 acttatatat cattactaaa gttaagactt taaagagaag cacttagatt acaatagatt
    24421 ttagatagct ttctttttaa aactattgtt gataaatgaa tgaatataag ggtatgtatt
    24481 aaaatagaga tactcatctt tttcattgtt taagtaatga aaatatattt tatttaatac
    24541 aaaaaagagt gccttataaa ctaaaagttc acttcgagac actcccttac attttgtttc
    24601 ttaaacttta caatattaca tacttattta ttaatttaat ctatgcatac ttaacccaat
    24661 aataaagtac atatatccaa ctcattaagc catgaaaaat agcccatgga atagatttcc
    24721 atgcagtata agaaataatc attgctaaac aagaaccaaa agaaataccc tttttaacag
    24781 ttgattttat agtattatta tttgaatcca taattaatcc tccaaaattc aaaataaaca
    24841 agttattata tatcttattt gaatctaata caatataaca gcttctaaat aaattatact
    24901 cttattatac ataatttata aattataatt ccgataatta tatgaattaa ttaattatat
    24961 taacctaaaa atattatcct atctaaaagt tatatgatag atacaacatg tcaacacatt
    25021 gtaattctcc ttcaaagatt ggttctggat aattatctac aaaaaagttt ttaattttat
    25081 gtgaataaac aaatccaaac ttctcataaa aaggaactcc actttcagat gtaccaacaa
    25141 ccattgatga atatttttta ctataaacat ctaaaagatg ataaatcatc tttgaggcat
    25201 aacccatatt ttgaaaatgc tcatatgtag caatgttttt taattcacat atttctttgg
    25261 aaatttcatt tacaactgct atacaagtaa gttctttatt atcatacaat gcgtacatag
    25321 ttcctttatt gagatattta tcaatcattt cttcacatgg gtcagctaat attaaaaggt
    25381 caatttgact ttttttattt atttctatag tttttatttc tatcataagt tctccaccct
    25441 cactttattc aaaccttaat ttaacaaact tcttacagta cttatttagt tcattttgat
    25501 atatattttt actttttaaa atatagtgat aattccctag aatattaata ttaataataa
    25561 attataatat cagctctagc aattacctag aatatcaata ctaatattca tctataatat
    25621 tag
//
LOCUS       DAMKMI010000044        25277 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMKMI010000044 DAMKMI010000000
VERSION     DAMKMI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.045"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            59..1210
                     /locus_tag="PC429_001698"
     CDS             59..1210
                     /locus_tag="PC429_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3847208.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1328..1966
                     /locus_tag="PC429_001699"
     CDS             1328..1966
                     /locus_tag="PC429_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF3847209.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            1967..2989
                     /locus_tag="PC429_001700"
     CDS             1967..2989
                     /locus_tag="PC429_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF3847210.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            2979..3599
                     /locus_tag="PC429_001701"
     CDS             2979..3599
                     /locus_tag="PC429_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847211.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3723..4409)
                     /locus_tag="PC429_001702"
     CDS             complement(3723..4409)
                     /locus_tag="PC429_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF3847212.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4423..4968)
                     /locus_tag="PC429_001703"
     CDS             complement(4423..4968)
                     /locus_tag="PC429_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847213.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5196..6443
                     /locus_tag="PC429_001704"
     CDS             5196..6443
                     /locus_tag="PC429_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF3847214.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFSVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6530..8524
                     /locus_tag="PC429_001705"
     CDS             6530..8524
                     /locus_tag="PC429_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF3847215.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8524..9846
                     /locus_tag="PC429_001706"
     CDS             8524..9846
                     /locus_tag="PC429_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF3847216.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10125..11411
                     /locus_tag="PC429_001707"
     CDS             10125..11411
                     /locus_tag="PC429_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF3847217.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11497..12804
                     /gene="grdB"
                     /locus_tag="PC429_001708"
     CDS             11497..12804
                     /gene="grdB"
                     /locus_tag="PC429_001708"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12544..12546,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF3847218.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDAVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13082..13813
                     /locus_tag="PC429_001709"
     CDS             13082..13813
                     /locus_tag="PC429_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847219.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
     gene            13815..15173
                     /locus_tag="PC429_001710"
     CDS             13815..15173
                     /locus_tag="PC429_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3847220.1"
                     /translation="MINLFSSDLFLKYSFLFSFIVYRILMVIVQYYFLISFTNVNSKF
                     WCFILYSIISIFLIIINTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSIVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMGLSSLITIIIAGFIYWYSSKKFNI
                     KVMNFDKYIAILMIPLLLIILFMQSFEYSSNINIDTSLGIVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDLSFSCNTQNIVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAIKACRVVDKQNRYLKLSTKLEGGFF
                     MIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQGAISIDKTSDYFQINVLLVISLHCA
                     SI"
     gene            15212..15697
                     /locus_tag="PC429_001711"
     CDS             15212..15697
                     /locus_tag="PC429_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847221.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(15960..16196)
                     /locus_tag="PC429_001712"
     CDS             complement(15960..16196)
                     /locus_tag="PC429_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847222.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(16351..16575)
                     /locus_tag="PC429_001713"
     CDS             complement(16351..16575)
                     /locus_tag="PC429_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3847223.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(16672..17925)
                     /locus_tag="PC429_001714"
     CDS             complement(16672..17925)
                     /locus_tag="PC429_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF3847224.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            18185..19174
                     /locus_tag="PC429_001715"
     CDS             18185..19174
                     /locus_tag="PC429_001715"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF3847225.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            19246..19986
                     /locus_tag="PC429_001716"
     CDS             19246..19986
                     /locus_tag="PC429_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF3847226.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            20202..21353
                     /locus_tag="PC429_001717"
     CDS             20202..21353
                     /locus_tag="PC429_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF3847227.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            21378..22133
                     /locus_tag="PC429_001718"
     CDS             21378..22133
                     /locus_tag="PC429_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF3847228.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            22325..24712
                     /locus_tag="PC429_001719"
     CDS             22325..24712
                     /locus_tag="PC429_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF3847229.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(24943..25176)
                     /locus_tag="PC429_001720"
     CDS             complement(24943..25176)
                     /locus_tag="PC429_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF3847230.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
BASE COUNT        10107 a         2651 c         4267 g         8252 t
ORIGIN      
        1 tgttttatta tagataatag agttttaagt atcttaaata atatttggag gaatatgaat
       61 ggaattaaga ccaagaagtg caaaattaga agaatttgat aaagtaatag aattaataaa
      121 ctatgtattt agaatatcaa gaaatcataa accaactatg atggaagaat ttcctctttt
      181 actaagtaaa aataatatag aaaatatgat tataatatca gaagatgata aagtggtttc
      241 tgatgtaaac tatctaattc aggatgtatc tattcaagga aacagattga aggttgcagc
      301 tataggtgga gtatgtactc atccagatta tgaaaagaga ggctattcat ctaagatatt
      361 ggataaagta gaagaaaaaa tgttttatga tggtgtagat attgtaatta tatcaggaac
      421 aagaagtcta tacagtagaa gaaattgtag tttggtaaag agtttttata aatatacaat
      481 aaaacctgaa gatgtaaaaa tagcatatga aatagttgaa tttgatgaaa ctaattttga
      541 aaaagataat gatttagata aaatgattga actatataat caaaatagta ctagatttat
      601 aagaacgaga gatgagtttc aaaaactatt gcatgcagca actattgcct ggggaccaat
      661 tggttacaag aaggtattta taaaggaaaa taataatata ataggatatt tgataataag
      721 aacaataaaa aaagaagatt ctactgtagg agaagttgct gaaatagggt taaactctgt
      781 aaatgttgaa aatatattga aatatgttgc aaataaattt ggattagaat ctttaaatta
      841 taaagtacat gtgaaaaatc ttaaagacca gttgaaatgt aatgggacaa aatctttgga
      901 ttatcaacaa ggaactatga aaataataaa ttttactaag ttgtgtgata gtttaagaag
      961 ttattttagt caatatgtag attttgaatt actaaaatac atggaattta aacaagttga
     1021 aaataaatat attattaaat acaaagaaga agaacttgta atagaaaatt tggataagct
     1081 taataaactg ttttttgaaa aaaatgagga acagtacaat gaatttaaac atctaaaaaa
     1141 tatatatgaa tttgctacaa aggcatttcc tgtagatttt ccatggactg caaatttaaa
     1201 ttatcaatag ttttagatga tattaaataa aattgattga tacctagagt tagtccttgt
     1261 taaataagag aaattattgg atattgaaaa agcaattttg taagtaatta gaaaggatga
     1321 gtattatatg gacatacttg atgatatagc agaatgtata ttaaatatgg gtgtagataa
     1381 tatagagaat ttagtaaaaa ttgctataga taaaaatatt gatgtggaag atatttatga
     1441 atatggttta aacaaaggta tgataggagc tttagataag tttgagaata aggaatacta
     1501 tctttcagaa gttatagtat gcacagatgc attaaataaa ggtattaatc ttttgaaggg
     1561 aactggaaaa gtaaagaaaa aatcaaaggg agtaattctg atgtctgtag tagaaggaga
     1621 tactcatgag ataggaaaaa atattgtaaa agtaatggta gaagccactg gatataaggt
     1681 tattgactta ggtgtaaata gaaaaagtga agatataata gaagaagcta taaaaaacaa
     1741 tgtagatata ataggcttat cttcaatgat gacaactaca atggaaaata tgaaatcggt
     1801 tatagacgaa cttaatatga ttgagataga taaaagacca aaagtaataa ttggtggagg
     1861 acctgtaagt atggagtttg cagaagaaat aggagcagat ggatacagtt caaatgctcc
     1921 aaaagcagtt aaattaataa ataaacttat aggaggtgaa gtttaaatgt tttcagatat
     1981 gactttactt cagtatataa atagtgaaga tagaggtttt tttctgcctg atatgggtac
     2041 aaatggattg ttcttaataa ataaaaaggc atatgaagta tatgatagtg tagatttgca
     2101 attagaatta gcaaagacta tggacaagta ttttgaatca gattttatat actcattctg
     2161 tgatgggatt acattttgtg aaactctggg tttagataca ttaagaccag attatgattt
     2221 tccaagtgta ttgacacata ctataactga cagagataaa ttaagaaaat tagatgttcc
     2281 agacccttat acgagtggac gtatgccact caatatagag agcttatcag ttatatcaaa
     2341 aagtattaaa aaaccactat atgaatcgat acaaggtcca tttacacttg caggtcaatt
     2401 agctggagct actcaacttt taagatgtat aataactgat aaaaaatttg tagaagaatt
     2461 attggaattt acaactgaat tggtaagaag atatgctgta gctgcaaata aagcaggagc
     2521 aaaatatatt tcaatagcag agccaacatc agtaacccta agtaaagata gatttgatga
     2581 atttatagta aaaaatttaa ataaaatcta caatgaactt gattgttgga aaggtatgca
     2641 tatatgtgga gacaccagag aattattaga taatatgcta agttgtaata tagatgcagt
     2701 aagtttagac caaattttag actatgaaga aattgctcca atgattccta gagatatagt
     2761 tttgattggt aatttagacc caataaaact tcttggacgt tctaagccag ataagattag
     2821 aagagagaca ctaaaattgt taaagaagat gagaagatat gataactttt tatgtgattt
     2881 tggctgtaac tgcttaaata ccactccagt agaaaattta caagcagcta taaaggcagg
     2941 aagaattagt tataaagaat tagataggat tgatattgat gaattataat agtaataaaa
     3001 ataaatatct tttaaaggat ataaatttag atgatatttt tatagataag aatattgtgc
     3061 taaagttttt ggggtatgga aatagaaaag ctccagatac aattagtaaa atcgttgaaa
     3121 gagaaataag aaatataaat gatattttag atatcaagat atatataagt gaagttgata
     3181 ttaataaaac acctaaagaa tgtaaaaaag catttgctgt actatataca ataggggata
     3241 aaattgattt taagatgaat gactatatgg aaaattgcaa tatgatggca gggctggcat
     3301 tggataaggt aggtatagta tgtttagatt gtattaatga gaaaataaaa atgtatttaa
     3361 aaggaaagta tgatagtctc aaaatatcgc atgaaatata tccaggagat aaagattttg
     3421 aagtagagag acaaaaggat atttataact atgtaaaaaa taaatataat agtatagaaa
     3481 tagaaattaa tgattatcat caactatccc ccataaaaag tgttgcaatg cttattttaa
     3541 tgggggatga agaaaattta gaatcaagat gtagtaagtg tcctagaaaa tgcttttagt
     3601 taattttcaa gactaagatt tgttttgtgt ataaattgca ataatttttt tggtcatttt
     3661 atgtataaac taaaaaaatt attgcaaagt aatattctat ttaatttgaa cagacttaca
     3721 aactatagta gtttctttaa ttcaataagt gtatttattt cataagttgg tatatgatta
     3781 gtataattta tactgttatt ggaatttaac caacaagtat ctattcctga atttattcca
     3841 cctaagatat cagaggttaa gctatcccca attattaagg cagaagattt atcatgacaa
     3901 ttaaactttt tgagagcgta tttaaatatt tcaggatttg gttttgatat tttcaactct
     3961 tcagatacaa ccattccatc aatatattct tttatttttg aattttcaag tcttttcttt
     4021 tgaactgcag aaattccatt tgttattata acaatagtat actttttttt aagatattgt
     4081 aatatatcga ttgcacctgg aattaaaaat gtacattcac ctaaaaaatt taagtacatt
     4141 ttgctaagag tttcagaatc aacatccaaa gatattttat ttgcaaaaag ttcaaatcta
     4201 agtaatttaa gttcattcaa agttataata tttttttcaa ggtcgagcca aagcttatca
     4261 cttatatttc tatatgactc tatataattc tcaaaattaa attctaaatc aaactcagat
     4321 agaagttttt taaaagcatg tgattcactt ttcttaaaat caaataaagt atcatctgca
     4381 tcaaaaaata taaatttata tgtcttcatg gtttttactc ctttacaaga tgactgcaaa
     4441 aattacttta ctttcactat ttgagtcatt ttgccattta tgttttgttc ctaaaggaac
     4501 ttttacacta tctcctttgg acaaagtaaa ttcatcatca tccataaaaa gtttaacttc
     4561 tccctccaaa acatatgcaa tttcatctcc attatgagta atcctatcaa cacaagaaga
     4621 agtgtttgga gctaaatcca taattgcaaa ttctaaatta ccactagagt ctggaattaa
     4681 aatctcatat ataacttctt tgcttccagg taaaataact ttttttctat tatcatgtct
     4741 tactatcaag ttttttttat ctacctcaga tacaaaaaat gtaaataaag gaacgtttag
     4801 agaagaggct atagatttta atgaatttac agatggatta gcaagacctc gttctatttg
     4861 gctcaataat gatggtgtaa catcagctag ttttgctaaa tctcgaatac ttaagttttg
     4921 tttttttcga acttcagcta ttttttcccc taaatttaaa tcattcatat aaatatcctc
     4981 tctttttaat ttaaaaaata ttaacttaaa ataatcaagt aaaatgatta tgtagtatga
     5041 aattattata acatatagaa ttaggttttg aaaaaactat gttcaatata attaaataaa
     5101 ttaaaatata attgacaaag tggatacaga atacttaaaa tatatttaaa taaaattaag
     5161 caaaagatag ttaatttatg ataggagggg atattatgca aataacgact tttggagcta
     5221 tatttggttt attaatagca attattttaa ttataaaaaa atttcaagct gtatatagtt
     5281 taatgctagg agcatttatt ggaggattag ttggaggagc aaatataaca caaactgtag
     5341 attttatggc aaatggagct atgaatatat caccatcaat tcttagagct ttggcatcag
     5401 gtgtattagc tggaagttta ataaaaacag gggcagtaga taaaatatca gaacagattg
     5461 taaaaatatt tggtgaaaag agggctttat tttcgattgc catttcaact atggtacttg
     5521 ctggagtagg cgtaaatttg gatgtatcta taataactgt agcacctata ggattatata
     5581 taggtagaaa attaaattat tctaaattgt caatactatt agctatgttg ggagggggta
     5641 aagcaggtaa tataatatca ccaaatccaa atactatagc agtagcagat aatttttctg
     5701 tcaatctctc tagtgttatg atggcaaata taattccagc aattattgga gttgttatta
     5761 cagttatttt agcaagtata ttaataaata aaggaaataa agtacaatca tatgaaattt
     5821 tagagcaaag agaagattta ccaagtttat ttaaatcatt atgtggtcca attatagcta
     5881 tatttctatt gtttttagga aatgtatctc ctatagttat agacccaatg atagcccttc
     5941 caataggagg aatcgtaact ctaattgtca caggaaactt gaacaattca agagaatatt
     6001 tagcttttgg attatctaag atgcaagggg tatgcatatt attacttgga acagggacta
     6061 tcgcaggaat tatacagatg tctgaattac aacagagtac aataggtgct ttacaatttt
     6121 taaatatgcc acaattttta ttggcaccag tttcgggtat acttatgtca ttagcaacag
     6181 catcatcaac agctggtgct actatagcat catctacttt tcatgatgct ataataaatg
     6241 gtgggctatc accgatttca ggtgcctcaa tagtaaatgc tgggtctagt gtattcgagc
     6301 aattaccaca tggctcacta tttcatacta gtgcaggtag tataaacatg gatataggtg
     6361 aaagatttaa gttaattcca tatgaagcat tgataggaat agtaatgact ataatttcaa
     6421 catctattca attagtttta taataaatat aaatatttaa tgaaaaaagt aggattttgt
     6481 tattaaatat tgaatttatt atagtaaaaa agaagattgg agttttaaag tgaataaaaa
     6541 aaagattgtt attataggga ttatttattc atttttagta gtattttcac ttacaaatat
     6601 gtatgtaaat atggagtata atctaaatgt atttgaatat attaagaagt cacttccatt
     6661 tacagaagaa gaaaaaaagt ggttagaaaa acacaagaat ttgatttaca gctctgacca
     6721 aagttcacct cctttgagat ataaaggtaa ggaagatgga cagtataagg gaattattgt
     6781 tgatttaata aattctctgt caattcaaat tggtagagat ttttatttta aaccaaataa
     6841 ttggtggaaa gaatctttcg taaattcaat agatgatagt ataaagtttt ttgacttgat
     6901 tccatcaaaa gaaagggcaa ataaatttat atttactgac ccaatataca ctttgagtgc
     6961 aaatatattg aaagataaaa agtcccaaga tataaatagt tacatggatt taaaggggaa
     7021 aacagtagca attccagaag gtgattattc tataaatttt ttaaagcaaa aaattcaaga
     7081 tataaacatt ttgttgactc cagatataaa gactggagtc aaccatttaa tgtctggaaa
     7141 agtagacgca gttgttggtg atgaaccagt attgcgatac tatataaata attatggact
     7201 ttcaaataaa tacagtgttt taagtaatcc tatatatact aaaaaagcag ttcttgctgt
     7261 acctaaacaa tatgaagagc tggttagtat tttaaataaa ggcattttta aacttcagaa
     7321 aaatggagtg tataaagatt taaagaaaaa atggtattct acgtataatg aagttgatga
     7381 tatattgtat gagcgaggaa ttgtgcctag tatttatctt tttataggta taattttaat
     7441 atcaatttat gttttttata gctatacata tttgttaaag atagaaatca aaaggaaaac
     7501 agaacaggta atagaaaata aaaaaacttt agaggcaact tttaatagta ttacagatat
     7561 aataatgctt gtagatgaaa ataacaatat agtagaatcg aacaaagtac tatatgactt
     7621 tatgggtgaa atgagttata agatagcaga tttgattagc atgataaaag gtgttattga
     7681 aaataccttc tctgaaaata ctaataaaac tagtgaaata gaaatacaca ataaaatttt
     7741 aaagataaac acttttcctg tagagtataa aaaaaataat actgagtata tagtagttct
     7801 tattaaagat ataactaatg ataaaatagt agaggcgaaa ttacttagag aaaacaaaat
     7861 gatatctata ggacaattag cttctggggt agcacatgag ataagaaatc ctttgggtat
     7921 aataagaaat aattgctatc tcttaaaaga taacgttaca atggaagaag taaatgattg
     7981 tgttaaatca atagaaagta atgtagatag ggcaagtaat ataataacca acttgttaaa
     8041 tttcgcaaga atatcagatg ataatttaga acatataaat ataagaaatt ttattgaaaa
     8101 tatagtgaaa cttcaatata aaatgttaca gttaaagaat gttgaaataa aaattgactg
     8161 tgaacataat ttagtatgct atataaatgg agaatcgtta aaacatgtat ttataaattt
     8221 aatttctaat tcaatagatg caatacatca agatggaaaa attattatat actgttatga
     8281 aaaaaattat tgtttattta tagattttaa agataatggt gaaggaataa aagaagatgc
     8341 cctaaaggac atatttaatc ccttctatac aacaaaacca attggtgagg gaactggttt
     8401 aggtttatac attacttata atgagattaa aaagaataat ggagatataa gtgttgaaag
     8461 taaattgggg gttggaactt gttttcatat aaaaattcca ttaaataaag aggtgactat
     8521 ataatgaaaa tactattagt tgatgatgag ctagaatatg gagtggtaat gaaaaaaatt
     8581 ctacaaaaga agggctatct tgtagatata gcactaagtg gtgaagaagc tataaatata
     8641 attaaaaaag ataaaaatta tgatttggtt ttgtctgatg ttatgatgaa aaatatggat
     8701 ggagtacagc tattagatag aattaaatct attaatagag atatagaggt tatattagtt
     8761 acaggatatg gaagtattga aaatgcagtt gatgctatga aaagaggagc tttatcttat
     8821 tttataaaaa gtaatcccat tgaaaattta ttagaagaag ttgaaaaggt aaaggcaagt
     8881 aaaatatctg tttctgtaca aaaaaataat ctggaattta ctttggaatc taaaaataga
     8941 gattttaata atatcataaa aatagcaaaa aaagctgctt gtaaagatgt taatatatta
     9001 atcttggggg aatctggtgt aggaaaagat atattagcta gatatataca tagcataagt
     9061 ccaagaaaga atgagatatt tgtacctgta aattgttgtt ctttttcaga aaatctattg
     9121 gagtctgagt tgtttggtca tgaaaaaggt tcttttacag gagcagtaga tagtagaaaa
     9181 ggtagatttg aactttcgaa taaaggaact ctatttttgg atgaaatagg agatatacct
     9241 ctaaatgttc aggtaaaact tttgaggaca cttgaggaca aatctataga gaggatagga
     9301 agtaataaat ctattaaagt tgactttagg ttaatttgtg ctatgaataa agaaccaaaa
     9361 gtggaaatat caaatggaaa tataagagaa gatttttttt atagaataag tacaataaca
     9421 ataactatac caccgctaag aaagagaaga gaagacttaa ctactcttat agaatttttc
     9481 ttaaataagt accaaattga acatgataaa aagatacact cgattgataa ggaagtaaag
     9541 gattttttat taaattacaa ttatccagga aatataagag aattaaaaaa tataataaat
     9601 agacttgtag ttttaagtga agaaggcaat ttatcaaaag acaatttaaa tttaatatca
     9661 aataatgtat acatagatga taaaataagt attagacctt taagagaaat aagaaaagag
     9721 tttgaatgtg agtatattga aaaagtttta tctttgtgtg gaaataatat ttcaaataca
     9781 gctaaaaaat tggagattag tagaagacag ttaaccaata aaatatcaga gtataatatt
     9841 aagtgagaaa aaaacttctc attaagtgag aaaaaaattt ctcaaactgt attaaaagat
     9901 aaacagaaaa aatctgttta tcttttttta ttataatttt aaaaattaat atttacaata
     9961 attattgcta aattcaatat tggtgtaaat taaaaatgaa aatttctatg acattaatac
    10021 atagaatata tattttaaat aattggcata gaaaatgctt tatatattaa taaacgtaat
    10081 aaatactttt aattgcgatt aaatatatta agggggattt tcatatgaag ctagagctag
    10141 ggaatatatt tataaaaaat gttgaatttg gagaaaagac agaagtaaaa gatggagtac
    10201 tatatattag tagcgaagaa atagaaaaga tagtattaga agatgaaagg attgtaagtg
    10261 taaatgttga acttgcaaga cctggcgaat caataagaat agctccagtt aaagatgtaa
    10321 tagagcctag agttaaaatt ggagatgaaa gcaaaatatt tcctggaatt ataaataaag
    10381 tgaagactgt aggaagtgga agaacccatg tacttttagg agcttgtgtt gtaacttgtg
    10441 gaaatatagt tggatttcaa gaaggcgtaa ttgatatgag tggtcctact gctaaatata
    10501 cacctttttc aaaaacaaat aatatatgta ttgttataaa agcaaaggat ggtatagata
    10561 ctcatgatta tgaagaagca gctagaattg caggattaaa aataggtgca tatgtaggtg
    10621 aggcaggaag agaagttgag ccagatgaag tcattgtata tgagacaaag ccattattaa
    10681 aacaagtaaa agaatatcca gacctgccaa cagtagcata tgttcacatg ttacaatcac
    10741 aaggactttt acatgacact tattactatg gggtggatgc taaacagata gtgcctacat
    10801 ttatgtatcc aactgaaata atggatggag ctataatatc tggaaactgt gtagcaccat
    10861 gtgataaggt tacaacattc catcatttaa ataatccagt tatacatgac ttatataaaa
    10921 gacatggaaa agatttaaac tttataggag ttattttgac taatgagaac gtattcttag
    10981 tcgataaaga gagaagctct gatatggtag ctaaattaat agaattttta ggagttgatg
    11041 gagttctagt gacagaagaa ggttatggga atccagatac tgacctaatg atgaattgta
    11101 gaaaatgtag tgaagttgga gcaaatgtag ttttgataac ggatgaattt cctggaaaag
    11161 atggcaagag tcaatctata gcagacgcta ctaaggaagc agatgcagtt gtttcttgtg
    11221 gtcaaggaaa tctagtggtt catttcccag caatggaaaa aattatagga actttagatt
    11281 atgttgaaat gatgataggt gggtataaag gatgcttaaa tgaagacgga agcatggatg
    11341 cagagctaca aattataata gcatcaacta tagcaaatgg ctataatcat ttgacagcaa
    11401 gatattatta aataagtatt tgaatataaa gttctgttga tataatcatt ggtctaattg
    11461 atgtaacaac tataaagtga tttggaggat taagagatga gtaaaatcag agtggttcat
    11521 tatataaaca atttctttgc tggtataggt ggagaggaaa aagcagatat tccgccagaa
    11581 aaaagggctg gtgtagtagg acctggaata gcatttcaga gtcaatttaa tgatgatgca
    11641 gagataatct caactgtaat ttgcggagat acgtattttg gtgaaaatat tgagagtgca
    11701 actcaagaac ttttaaaaat gataaaagaa gaaagccctg atttatttat tgcaggccca
    11761 gcatttaatg caggtagata tggagtagct tgtgggacta tttgtaaggc agttgaagaa
    11821 gagttaaata ttcctgtaat tacaggcatg tataaagaaa atccaggtgt agatatgttt
    11881 aaattagatt tgcatatagt gtctacagga aattcagcag caagtcttag aaaggtagtt
    11941 ccaataatga caaaactagg tcttaaattg gttagaggtg aggaaatagg acctcctgaa
    12001 gaggaaggat atataatgag aggaataaga aaaaatttct tccatgaact tagagggtct
    12061 gaaagagcaa ttgatatgtt ggttaaaaag atgaaagggg aaagctttga aactgagtat
    12121 ccaatgcctg aatttgatag agttacacct atgaaacctg ttaaagattt atctaagata
    12181 aaacttgccc tagtaacttc tggagggata gttccaattg ataatccaga caaaattgaa
    12241 tcttcaagtg ctactaaata tggaatttac gatttaacag atatggattc tatgtcaaat
    12301 aaagaattta caactataca tggggggtat gacagagcat atgtccttga aaatccaaat
    12361 ttagttgtac cactagatgt agttagagag cttgaaaaag aaggtgtaat tggagaatta
    12421 gtagattatt ttataactac tacaggaaca ggaacgagtg ttggcaattc taagagattt
    12481 ggagaagagt tttctaaaaa attgttagaa gatgatgtag atgctgtaat tttaacttct
    12541 acatgaggta cttgtactcg ttgtggagca acgatggtaa aagagattga gagagctggt
    12601 atagcagttg ttcatatatg tacagtagta ccaatatcac ttacaatagg agctaataga
    12661 attgttccag cagtagggat accatatcca cttggaaatc ctatgttagg agaagaggaa
    12721 agtaaaaaaa ttagaaagaa tatagtttta aaagctttaa atgcactaac agaagatata
    12781 gaagaacaaa cagtatttga ataataaaaa tacaacacaa cactgaaata aaaaaagata
    12841 tcaaaaaata attttttaaa attattttac gatatctttt ttttattagg taaattcgaa
    12901 atttgcattt tttaaaatta aatttataaa agtgataaat tagtttatta taatattgat
    12961 acacaaattc aaattagata tactatgaaa agcgagatta ttacattttg gaacaaatat
    13021 tcaacatatt agatcatatt aattgaacag aaaaaattaa ctttatataa taattagaat
    13081 aatgataaga aagaaggtgt atatattgat ttcaattgcc atttgtgatg atgaatccta
    13141 tatgctaaat catttaaagg aaaatataga attatacatg aagcagaaga atttattata
    13201 taatatagag ctttttaata aagtagaaga tttactatgc aatcatgaaa agtttaatat
    13261 tgtattttta gatatacaga tggaagagat taacggaatg caggcagcta gaaaattaag
    13321 gtcatatggt aatgagtgct ttataatttt cgttacagtt ttaaatgaat ttgtatttga
    13381 tgcttttgaa gtagatgcta taaattatct gataaaacct ctatctaatg agaaactata
    13441 taaaactttg gataaaatag ttacaaaact tgatgataat gaaagtaatt atatcacaat
    13501 acaaaaaggt tattcatatt ggaaattaaa acttgctgat attttatatt gtgaagttat
    13561 aaaaagaaag atttatgttc atttaaagaa agagactgta gattattatg agactattga
    13621 aaatttagag agaagtttac caacaaattt ttttaggtgt catcgtagtt atataatcaa
    13681 tttacaatat gtatatggat atgaaaatgg taatgcagtt atggaaagtg gagatagaat
    13741 acctgtttct agactacgtc aacaaatatt ttcaaaagtt atgcttgagt tcatgaaaaa
    13801 taggaggata taagatgatt aatttatttt cttccgattt atttttaaag tacagttttt
    13861 tattttcttt tattgtatat agaattttaa tggtaatagt gcaatactat tttctaatca
    13921 gttttacaaa tgttaattca aaattttggt gttttatttt atattcgata atttcaattt
    13981 ttttaatcat tataaataca tattttaatt tatataattt attttcttca cagattctat
    14041 tttttcttat gactttttta tttgtaaagt acataataaa acaatctact gttttatcta
    14101 tagtatcaac tttgttagta tatttaattg aacaattagt atatggattt acagctccat
    14161 taaattactt gatatttact agtgatataa atacagatat ttataaattt gatataaata
    14221 tgggactatc ttctttaatt actattataa tagctggatt tatatattgg tatagttcta
    14281 aaaagtttaa tattaaggtc atgaactttg ataaatatat agcaattttg atgattccat
    14341 tgttgcttat aatattattt atgcaatcat ttgaatatag ttcaaatata aatatagata
    14401 caagtcttgg gattgtaaag ctgctattaa atacttctat aactgaagaa attcaagctt
    14461 ttcttttttc tataattgga actatatgtg tatttttttc tctatttacc tttaagaaac
    14521 taattcaagc tctcgaagat gacaaggaga gggcaattat gaatcaacag attcatgctc
    14581 aaaaaaacta tattgaagaa gcgaaatcac gattatcaca aactatatcc tttagacatg
    14641 attttaataa tcatttagct attgtcaatg gattgttgaa aaaagaccaa attttaaaag
    14701 cacaagatta tttaaataag cttgaaaaag taacaagtga cctatcattt tcttgtaata
    14761 cacagaatat tgttattgat gctcttttca acaataaatt aagtatagca aaacaattag
    14821 gcatagaggt agattgtgaa gtaattatac cccaaagcac taacatgaac gattttgacc
    14881 tatgtatagt gtttgctaat gctattgaca atgcaattaa agcttgtaga gttgtagata
    14941 aacaaaatag gtatttaaag ctttccacta agttagaggg tggatttttt atgatagaaa
    15001 taaaaaatag ctataatcca ataaataatt atacaagtgg ctcaggtata ggtcttttaa
    15061 atataaaaga aattgctaaa aaatatcaag gggctataag tatagataag acatcagatt
    15121 attttcaaat aaatgtattg ctggttattt cattacattg cgctagtatt tgataacaat
    15181 ttcattgaaa atagtatttg taagctatat tatgtacttg aatagagaaa gtttgatata
    15241 tagaaatgag gaggttacta aaatgaaagt tggaaatata aatggtcaag caccaattac
    15301 aaatggttta gatagttgtc aaagtgaggt acaaggctta gaacaaaaga aaaaatcatt
    15361 acaggatgaa atagagaaag taagaagtga taaaagtatt gatggtgaaa cagccaaatt
    15421 aaaaatagaa gctttaaaaa aagaaatgga agagattgaa aataaaattc aagagttaaa
    15481 aggaaataaa gcaaaaaaaa caaataaaga agaaaataat gtttcagaag taaataataa
    15541 tctattagag agaaaaaaag aaccttctaa taatgaaaat gtaaaacaat tatatgatgt
    15601 ttttgaaaga agcaatgtag acgaacaatg ggataatact tatcgtgttc tccaagatga
    15661 taaaaactta aaagtaaaat ttaatagacc tatatagttt atttataaaa atagttaata
    15721 ttttaattat caccactttt tagaagaata cacactttat tttaaagacc cacatattta
    15781 tttacagtta gcacatttat atgtggtttt ataactatta tttacaagtc tatattgata
    15841 acttttacat agtatataat gtttaaatac ttattcaaat ttatctatac ataaacaaaa
    15901 aaagagattt taaaatctct ttttagattg ttgacttatg gctttttgat ttaataaaat
    15961 caagaagccg tttgaatttt atctgttggc tgataccctt ctgatattga aacaccattt
    16021 ttgtttccaa aatcgtttaa aattaagaac ttattatgaa tgactatttc attatgaaca
    16081 ataagcagat aacatgaatc ttcaatcaat gttttaacag tagcattaaa tgcttgctta
    16141 tacagttcta tcgtttgttt gaactgtcca ttaaagtaaa tatgagtcaa taacataact
    16201 atatctacac aaattaatat aaatttttta tactgtacat tatctaactg ttgattatct
    16261 gtattacaac atgattgaat ttaaaaagcc atcaacacat agaatgttga tggcctgaaa
    16321 atagatttaa aatctctttt ttattatata ttaattattt tttaaattta tattttttgc
    16381 atcagatttt ctaatagcta agtcaaaccc tctaaatcta atcactatag ggtcattaag
    16441 aggagctatc tttttcactt caacttcagt gccatctatt agtcctaagg ctagaagtct
    16501 cttcataagt ttttcatttc cagctatatt gtctataatt cccttttgtc caagttttaa
    16561 gttgtaaaca gtcattatta acacctccta atgaaaatca ttatcaaact ttatatttat
    16621 aatatataac aaaaagagca aattgtcaat atataacaaa aaataaacat actaagttag
    16681 tatgtttata aaaaatagta tacatgggat actaatagca tctagcaaaa aaccacttac
    16741 taatggtaca actaaaaagg cttttggaga atcaccatat ttttgtgtca aagaactcat
    16801 atttgccaga gcgttcggag tagctcctat tccatgtcca atcaaaccac ttattatgat
    16861 ggcagaatca aaatcttttc caagtactct aaagcaaata aatattgaaa ataggattat
    16921 aaagattact tgaaaagtta ctattaagag aataggaggt aataatgtag ataattcaaa
    16981 taaatctata ttcataagtg ataaagttaa aaaaattccc aatgaaagtt cttttaaaaa
    17041 atctataagt ttaaaattta attctagtat atttatctta tcatttaaat ttctaaaaat
    17101 aacagccgaa aacatacaag ttacaacaga gggtaatagt atattaaacg ttatatagaa
    17161 gcatctactt ataatttctc ctgtatttat acaaataaat atgagaagaa cctgttccaa
    17221 aaatacagat ggtgttattg tttcggagaa gttattattt tttatcctat tcttatttct
    17281 aaaaaattta cttccaaatc tctttagatt tctattatat ctaggaatcg acaagtctat
    17341 atttgatgat ggttttaatt tatatttatt tattagaaat ttagctacag gacatcccaa
    17401 tataccaccc ataataagtc ctattgttgc agcagataat ccaaccccaa ctgctccttc
    17461 tataccgaga ttttctattg taagtccaaa tgcagcagaa taaccatgac caccttccat
    17521 agatacattt ccacacataa gccctaaaag aggttcaagt tttataatct ttgatattgt
    17581 aatagctaat atattttgac aaaatgccaa tactccacac aaaatccaat atctaaataa
    17641 tacactacca cctttcttta taagatttat gcttatttca agtcctatag ttgtaaaaaa
    17701 gaaggttatg aaatttggca ttaaatttcc acttatagat atatctgcta tattaaaata
    17761 tttaaaaaat aaatttagaa aacaaaataa tagacctcct attactggtg caggtataca
    17821 gaaattattt aaaaagttaa ctttattctt caataaatta ccaattaaaa agaagaatat
    17881 agatagtgtc aaggtttgca ctatatcaaa acttaaggta atcaaaataa gtcctccttt
    17941 gtaattcaaa gttttaatta tataacttaa attcttatgt attgaaattt tattatcaaa
    18001 tgtatattaa taaatgtata ttaaaaagaa tcagtagata taattagata gtcataaatt
    18061 aaattaaaat attttaatga gatatgactt ttttagaatt aaaaaccgta gtatattgat
    18121 ataatagaaa taagacttaa aaatctgttg atagtattat ttataagtac agtaggagga
    18181 taaaatgttt gaaaatttac ctaagataga tttacattgc cacttagatg gaagtgttag
    18241 agtagaaacc atgcttgata tagctattaa agagaaaata gatttacctt caaataatat
    18301 ggatgaaata aaaaaattag caaaggtttc ttttaattgt acttcactag atgaatattt
    18361 agaaaaattt gatttaccct taaaagtaat gcaatctaag gaaaatttaa aaagaattac
    18421 ttttgaactt ttggaagatg caagtagaga aaatgtaaaa tatattgaaa ttagatttgc
    18481 accattactt cacacccaaa aaggtatgag tgttaaaaat ataattgagg gtataattga
    18541 aggaattaga gaagctgaaa gtatttatga tataaaaggc aatttaatat taggttgtat
    18601 gagaacaatg acctcaaaag aggcattatt agtaattgaa gaagggaaat catttgtaaa
    18661 taaaggtgtt gtagcagtag acttgtgtgg tccagagaaa gagggatttt gtaaggaata
    18721 taaagatgtt tttaaattag caagagaata tggatataaa gttacaatac atgcaggaga
    18781 agctgcaagt ggagaaaatg tattggatgc tataaatatt ttaaaggcgg ataggattgg
    18841 tcatggggta aaaattaaag accataaaaa ggcgtataat ttagttaaag ataaaaaaat
    18901 attgcttgaa ttatgtccaa ctagtaatgt acaaactaag acagtagatt cttatgaagc
    18961 acatccattt tatacttttt ataaagataa tttacatgta agtataaata ctgataatag
    19021 aactgtatca gatataaatt taagttcaga actaaatgtt atatttgata cttttaaact
    19081 aggattagaa gactataaaa taatttatag aaatgcagta gaagctagtt ttgcagataa
    19141 agaaactaaa gagtatttga atagtttaat ttaatagata aaaatattta aactttaaat
    19201 taagctatta aattgataaa aatacattgt attaggagaa aaaatgtgaa agattatgaa
    19261 gatgttgttt atattaaact agtcaaaaaa gaacttgaag aatggaagaa aaatatatta
    19321 aagaaacctt ctatatttga caaagcatca aagggtatac agaataaatt taatggagta
    19381 ttgccagata aatatcatga aataattact tcttctataa aagtaatgac aaaaaccgta
    19441 ctgtttggat ataaatatgc tacaaagcca ccacttagaa atatgtcatt agaagaaaga
    19501 gaaaaattag ttttagaaaa agctaaaact tacaagacta cagcaatgat tgaaggggca
    19561 ggaacagggg caggaggaat cctcttaggt ttatctgatt ttcctttgct tttaggtatt
    19621 aaagtgaaat ttttatatga tgtagcttca atatatggat atgatgttaa agattataga
    19681 gaaaggattt atatattaaa tatattacag ttggcttttt ctagtcaaca gcatataaac
    19741 aaaactttta aattgatgaa taactgggat gaatatgtaa atagtctacc agaagacatc
    19801 aatgattttg attggagaac atttcaacaa gagtataggg attatttaga tttagctaag
    19861 tttttacaac taatgcctgg aataggagca tttgtaggat tttatgtgaa tggaaaatta
    19921 attgataaat tatgtgaaac tgctgtgtct gcttatagat tgaggattcg agagcttaag
    19981 ttataagaaa aattataaca taaaaaatta gttatacaat atatctatat gtatctaatt
    20041 ttgcattgat aaaaacaatt atggataaaa ggtaaagcat gatagaatct aatgtagagg
    20101 tttgtatttt aatggaaaaa tttagttttt attgcttaag atacaaattg aattacgaag
    20161 agattgaatt atgaagagaa gtaattggga ggaatatttt catgacaaat ttgccaaaac
    20221 aatttgatag cttcaatgaa gctagacaaa aaggatttat aaatgcaaag gaacttaagg
    20281 aaagtgggaa aaagatggtt ggagttttct gtacatttac tccagttgaa attccaatgg
    20341 cagctggagc aactgtagta ggtgtatgcg gcgttagtga agagccaata cctgatgcag
    20401 aaagggtgct tccaagaaat ctttgtcctt taattaaatc aagctatgga catgcaataa
    20461 cagatacctg tccttatttc tatttttctg atttgttgat aggagaaacc acttgtgatg
    20521 gtaagaaaaa aatgtatgaa gaacttgcaa aagttaagcc aacatatgta atgcatttgc
    20581 caaatacttc aaaaggagaa tttgcctata aactttggaa agatgaaatg ataagattaa
    20641 aagaagaggt tgagaaatct ttaggtgtaa ctataactga agaagatata agaactgcta
    20701 taaaagataa aaatgaagaa agagaacttt taaaagagtt ttatgcactt ggtaaattac
    20761 aaccatctgc acttacaggc cttgagttac ataatgtttt atatcaagca ggttttaaat
    20821 ttgaccgtgc agaacttaaa cattctttaa gaaaagttat agatgatatg aaagaaagat
    20881 atgaaaaagg tgaatgtcct gttcaaaaag ataagccaag aatattgata acaggttctc
    20941 ctattggtgg aataagcgaa aaaatagtta aaactttaga agatgcaggg gcatcagtcg
    21001 ttgcatatga actttgtgga gctattagat caaacgattt attggttgat gaagaaatag
    21061 aagatgtata tgatgcttta acacaaaaat atataaatat aggttgttct tgtatgatga
    21121 ataatgacaa tcgtattgaa ttattagata gaataataga tgaatataat gtagatgctg
    21181 taatagatgt agttttacaa gcatgtcata catttaatat agaaagttat agaataagag
    21241 aatttgttac aaaagaaaaa aataagccat ttatgtctct tgaaactgat tattcaaaat
    21301 cagatactga acaattaaga actagatttg aagcgtttgt agagatgttg taggaataaa
    21361 taaaagtagg aggaactatg tttagtatag gagttgactc agggtcagtg gcaacaaagg
    21421 gtgtgctatt tgatggtgaa aaaattatta agaaaataat tataccaact ggatggagcc
    21481 ctaaaagtac atctaaacag gtctatgagt tgctaagtag tgaaattgac aaaaaagata
    21541 taaaaaaggt ggtaggtaca ggttatggaa ggggagtaat ggattttgct gataagaaag
    21601 taacagaaat aacttgtcat actagaggta tttacttttt aaacaagaac ataaggacta
    21661 ttttagatgt aggtggtcaa gatagtaaag ttataaattt agatagagat ggaaatgtat
    21721 ttaactttat aatgaatgat aagtgtgctg caggaactgg tcgattttta gagattactt
    21781 ctaacctttt aggaagcgat attgaaagta tagatacatt agctaaagga tatgaaccag
    21841 ttaatatatc tagtatgtgt acagtttttg ctgagtctga aattgtaagt cttctagctc
    21901 aaaacatttc tactggagag gtagcagcag ggatacttaa atctattgca aataaatcca
    21961 cctcaatgtt agcaagaggt gaggtaatag atgaagttgc atttactgga gggcttgcaa
    22021 aaagtaaaga actagtaaaa atgatagaag aaatactggg taaaaaaatt tttattgcag
    22081 aagatactca aataatagga gctttaggag ctgctgtaat aggatttaga taaaaacata
    22141 taaaaagaaa atgagaagct gaatttatta taaataaatt cagtttttta ttttatttta
    22201 tttatttata aaggaaaact ttggtataat tgatttaaat gattgtatat ttgtaaatac
    22261 ttgatattta gtcatatata acattataat tttaaaaatt agctcaaaat aagggggaga
    22321 aagcgtgaaa aaatttactt caaaaaaagt aacaaggttt gttgcgctgt ttttaatatc
    22381 tattttagta atgtcaacaa ttcctgtatc agctgacaat agcaatacat tgaatcagat
    22441 aaaaaaagca gaatacagtg agacgtataa acaatatcta aatgatgcta aaaatggatg
    22501 tacagaaaaa tataatggga ttataccaaa tccatatcag ttggaaggta ctcaaatcca
    22561 aagtaaagga agaacagctc caacatctta tgaccctaga aaattaggtc ttatgacatc
    22621 aataaaaaat caagaggatt taggaatctg ttgggatttt gctgcaatgg caaccttaga
    22681 aagtttctta aagttaaata attatggaga ctatgattta tcagaagaac acttaagatg
    22741 gtgggcttca gatggtgaat atggatggtc tgtaaatgat atgaatggtg ctttaaatta
    22801 tgaagctatg ggatatttaa catcttggtc aggtcctaaa ttagaaaaag atatcccata
    22861 caatggtaga gtatctaaag cacaaggagc aaaaaaacca actaatatga atacagcacc
    22921 tactgttttt aatgtgacag atgctgtgtg tgtaagcaat gacataaatt ctacaaaaaa
    22981 tgctatatta cagtatggag ctgtaacatc tggttattat gaagatatta aatatcaaag
    23041 tgatgaccaa aattcatatt attgtccaac aaaaagtttt aatacaaatc atgctatttc
    23101 aattgtaggt tgggatgata actattctaa agataatttt aatgcaaata ttagaccatc
    23161 aaaaaatggt gcttggttaa taaaaaatag ctggggagat tataattctg agggtggata
    23221 tttctggata tcttatgaag acaagacact tatgtctgat atagataatt tttcaatcaa
    23281 aggtggtaaa aaaccaaatg atgatgagaa gatgtatcaa catgactatg caagtattgt
    23341 acctttaatt tcaaaaaaga taacagctgc aaatgtattt gatttcaata gaggcgatga
    23401 aactctaaaa tctgtaatgt tcttaactga aagtatagga gctaagtatg aagtatatta
    23461 tgctccagta gtaaatggta tacctcaaga aaacaatatg aaaaaattaa aagaaggtac
    23521 tgcccaatat tcaggataca taacagttcc aattgattct tttgatatac cagaaggaaa
    23581 gggtgcaatt gtagtcagta tagaggggaa aaatggagag tcaactatag gttctgagtc
    23641 aaatgtacca ggatatgata catttaaagc aaaagctaat ttaggtgaaa gttatatcat
    23701 agataatact ggaaaattct ttgatataaa tagagatagt aacttctatc cgtgtaattt
    23761 tactataaaa gctgttacag aaaagtcttc tggtgagagt atcccaaatg agtctttaat
    23821 tggtagtgat agatatgaaa ctgctatcaa agttagtcaa aatggtttca attcttcaga
    23881 aaatgtagtt ttagtaaatg gcagttcaat agtagatgca ttggcagcaa ctccttttac
    23941 atctgctata aattcaccaa tattattgac acaaaaagaa gctttaaatt caaaaactaa
    24001 agctgagata cagagattag gtgctaagaa agtataccta ataggtggag aaaattctat
    24061 aagtaaagaa attgaacaac aattaaaaag cttaaatata tcgatagaga gaatatcagg
    24121 aagtgataga tacaagacaa gtttattatt agctcaaaag ttaaatggta taaaaaatgt
    24181 atctcaaata gcagttgtaa atggagtgaa aggattagct gatgcaatta gtgtaggcgc
    24241 tgcagcagca gaaaataaca taccaatcat acttgcaaat gaaaagagcg aattgcaagg
    24301 agctgatgaa tttctaaatt cattaaatat agagaaatct tatattattg gaggaacagc
    24361 atccctatca aacaatctag aaagtaagtt aaaaaatcca actagattat ctggtagtag
    24421 tagagacgaa actaattcaa agataataga taatttttat aaaaaagata ctttaaaaaa
    24481 tgcatttgta gttaaaaatg gaatcaagaa tcaaaatgat ttaatagatg gattggcagt
    24541 tggtccttta ggagcaaaaa ctgggtcacc agtaatttta gttggtgata aattagctga
    24601 tagccaaaaa gaagttctta aaaataagac tttagaaaaa gtaactcaag ttggtggagg
    24661 agcaaataaa aatgcattta aagagttagt aaaattaaaa agtgctaaat agagtactta
    24721 agtattaaca tatattagtg tgtattagta acttatctta ataaaagctg aaaataatta
    24781 cttcaaatta ttgataaata agataatcaa tgattgtaaa gttttgtttt cagctttaaa
    24841 tttatgtagt ataagtttaa aaacataaaa aagggttact ttatatagaa atatagaaaa
    24901 tcttacattt tatatataaa taacccatat ttaattaact ttttatagtg ttctaataga
    24961 tattattgaa cttaatggta aatataatgt gtaagtttca tttaaagttg gattagattc
    25021 atctatatat cttgagcaaa gtgtaattga taagctatct gcttctataa tttcaccata
    25081 tagaggacaa ctgctaaagt aagaatgtac ttcaacatat ttatttaagt tttcttgaac
    25141 ataatcgtaa aaacggtttt ttgtagtagt agccataaaa tcaactcctt tattttttat
    25201 taaagttgaa acttttaaaa agataaatat gattacttct attagaaact aagttaaaaa
    25261 ataagcaaat agatatg
//
LOCUS       DAMKMI010000045        24553 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMKMI010000045 DAMKMI010000000
VERSION     DAMKMI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24553
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.046"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            87..1964
                     /locus_tag="PC429_001721"
     CDS             87..1964
                     /locus_tag="PC429_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3847231.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSRGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2206..4119
                     /locus_tag="PC429_001722"
     CDS             2206..4119
                     /locus_tag="PC429_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847232.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKVTTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4112..6331
                     /locus_tag="PC429_001723"
     CDS             4112..6331
                     /locus_tag="PC429_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF3847233.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLSLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6440..7270
                     /locus_tag="PC429_001724"
     CDS             6440..7270
                     /locus_tag="PC429_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF3847234.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7260..10727
                     /gene="addB"
                     /locus_tag="PC429_001725"
     CDS             7260..10727
                     /gene="addB"
                     /locus_tag="PC429_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF3847235.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10727..14554
                     /gene="addA"
                     /locus_tag="PC429_001726"
     CDS             10727..14554
                     /gene="addA"
                     /locus_tag="PC429_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF3847236.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14600..15811
                     /locus_tag="PC429_001727"
     CDS             14600..15811
                     /locus_tag="PC429_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF3847237.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15798..19325
                     /locus_tag="PC429_001728"
     CDS             15798..19325
                     /locus_tag="PC429_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3847238.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19456..20709
                     /locus_tag="PC429_001729"
     CDS             19456..20709
                     /locus_tag="PC429_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF3847239.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            20858..21910
                     /gene="ytvI"
                     /locus_tag="PC429_001730"
     CDS             20858..21910
                     /gene="ytvI"
                     /locus_tag="PC429_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF3847240.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            22000..23226
                     /gene="pepT"
                     /locus_tag="PC429_001731"
     CDS             22000..23226
                     /gene="pepT"
                     /locus_tag="PC429_001731"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF3847241.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            23433..24485
                     /locus_tag="PC429_001732"
     CDS             23433..24485
                     /locus_tag="PC429_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF3847242.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADNISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
BASE COUNT        10477 a         2359 c         4381 g         7336 t
ORIGIN      
        1 tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat aacgctgatt
       61 taaatataaa cttagagaaa aaaagtatcg ttttaggtag caaatcaaaa gttagtgtca
      121 aatttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc tatgacatgc
      181 cattggatac aactctaaat tacaatcaat caactggggc atatgaagga actatcaatt
      241 ataaccaaga cccagaatat ctaaatgttt gggaactaca agggataaca ataaacagca
      301 aaaataatcc taaaacttta aacagacaag acctagaaaa gctgggatta aatttaaaag
      361 actataatgt aacacaggaa tgtataattg aagacataac ttctagaaaa gatgtaaata
      421 aatatttgag aaaaacttct tcacctatta cagaacttac aggaagtgat agatatgaaa
      481 cagcagttaa aataagtaaa gagggttgga aaaatggttc agataaagta gttataataa
      541 atggggatgt aagtatagat ggcattatat caactccact ggcaaccaca tataatgctc
      601 caatactttt ggttgaaaaa aacaatgtgc ctaatagtgt aaaatcagaa ttaaagcgcc
      661 taaaccctaa agatataatt ataattggag atgagaatgc tatttctaaa actactgcta
      721 atcaaattaa atcaactgta aatgctagtc aaacacgttt aaatggttct aatagatatg
      781 agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa aaaatataca
      841 taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa gcaggtcaag
      901 acaagcaacc aattatatta actgataaag atagtattac agacaataca tataaatggt
      961 taaagagtga ggatttacaa aatgcttatt ttataggtgg tcctcaaatg atatcaacaa
     1021 atgttataaa taaggtaaat ggaataacta aggatagtgt tactaataat agagtatacg
     1081 gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca gatgatgagt
     1141 tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca gcaggtccat
     1201 tggctgcgaa cttaaaatct ccaatactta taacaccaaa gacgtatgta tctgcatacc
     1261 ataaagataa tttagaagct aaatcagcta ataaagtata caaaatagga ggaggattga
     1321 cttctaaggt aatgagctct atagcatcat cattatctaa acacaatacg actccaacag
     1381 aaccaggaaa tagtgggggc aagacagtta tgattgaccc agggcatggt ggttcagcac
     1441 ctggaaattc atctagagga atgattgaaa aagattacaa tttaaatact tcacttgcaa
     1501 caactgaata tttacgttca aagggattca atgtaataat gacaagagac acagataaga
     1561 ctttatctct tggaaataga actgctctat ctaattcatt gaaaccagat ttatttacaa
     1621 gtatacatta taatggctca actaataaac aaggtcatgg tgtagaagta ttttataagc
     1681 ttaaagataa aaatggaggg actactaaaa ctgtagctac caatatatta aatagaattt
     1741 tagagaaatt taaacttaca aatagaggta taaaaacaag agtacttcct agtgattcta
     1801 caaaagatta tttatacgtt ttaagaagta atgatatgcc agctgtactt gtagaatgtg
     1861 catttttgga taatgaaaat gatatgagtt taataaactc atctgcaaaa gtaaaagaaa
     1921 tgggtacaca aataggtaaa ggaatagaag attcattaaa ataaatggaa ataaaaatat
     1981 ttaaaaactt aatgtaaatt tatagttgta aatttatact aaagtcaaaa aatgaaatat
     2041 aagtgtatca atagtaagac tgtatgtaca tttgcacatg tgaatgcaag tatatataca
     2101 gtcttttttt gtagttaaat gcaataattt gattgtgttt tgtaaaatac acaatatgat
     2161 ataattagat aatatacact taatttaatt gaggaaggtg attatatgga atatttattt
     2221 gaaaaaagta gtttaaaaga atatggaaat gaagctttag ataaatatag agaattatta
     2281 gataaaactg gtaataattc aaggtgtatt cttttgttag taccaaacaa taacacaaag
     2341 atacgatatg aaagggctct aagattaaat tatagtgaag aactgaaagt aacaacatat
     2401 ataagttttg tgaagaaaga attagttaaa tactggccac taataataga aaaatgtgat
     2461 ggaataagta caaaggttgt atctccaaca tttatatcaa atagtttaag tgaatattta
     2521 atagtgcaaa aagttaagga aaaaaggata caagaaggat attttgaaga tattacagga
     2581 accaataaat ccatagcaaa tagtataatg actaacataa ataagtctgc atttaattta
     2641 attgattttg attttattgg agaaaaaata tattcatcaa agaaaaataa agatagtata
     2701 tatagatttt catacactca aatggatgaa attatatcat attatataaa ttccttacta
     2761 tcaaattcaa ttttagataa tgcattaagc atatatttat acaatcaata cctacttaat
     2821 gatgattttt atttaaaaaa cttatttaca gaagtgagat atataattgt ggatagcctt
     2881 gaaagctcaa gtaatgcaga agttgatttt ataacagaag cattggataa tacattacaa
     2941 agttatatat attttgatta tagtagggat tattctgtat ttaataatgt ggatatagac
     3001 tatataaatg agaaaataat atcaaaaatt aagcctaaag aagaaaatat taaaaagaag
     3061 gagattagaa tagaagattt atacttatta cctgcaaata tagaattaaa tcaatcaagt
     3121 caattatata atgaaatgtt agatttaata agtgaaaaag ttatatctct tatagaagaa
     3181 gatgtatctc caagagatat agcaattata tctcctataa acaatagtat attggagcat
     3241 caaattagag attctttgat agaaaaaaat atagatgttt ttagtactaa gaaggataaa
     3301 aaagctgtgg attatccata tgggaatgca ttggtagtgg ctacatgtat attctatgga
     3361 tatttagatt ttataaaaga tgatgaatac ttaagctttt tagaaactct tttagaaata
     3421 aataggataa aagcatttaa gatttttaat gaaacaagac atttaagtat tgataatata
     3481 gatgaacata gtcagtatcg tgatatttta cagtatattg aagaaaaaaa gaagagtgat
     3541 ataaaaatac atgaattttt aactcaattt tacatagata aaatgttaaa tttaaaagaa
     3601 ggtaaaaaga atgttgggtt atgtaaaaag ataatagctg aaagtgagtc ttttagtgaa
     3661 agtttaagtc tacttgggat gaaaaaagaa aagatatttg tagaagcttt aaaaagtaca
     3721 ataaacgatt attactctgt tgtcgatata gaagagttga aatccaaaga caaaatagta
     3781 ataactactc cttattcata tatatcatca aatatagata gaagtataca aatatgggta
     3841 gatataggaa gtaatgcttg gaatatgaaa atagaaaaag atataagtaa tttaattgta
     3901 cttagaaaat cttttgaaga aaaaaagatt tatacaaatg aaatggaaga atattataaa
     3961 aaatattatc tatacaatac agtatataat ttgttattaa atgcaaagaa aatctatgca
     4021 tttaaaagtg aatatgcgat taatggttat atccaagaga gtatattata tagtatactt
     4081 ttaaaaatct cacataaagg agataaaaat tatgattaat ataaattata gagaagacca
     4141 gataccaata ataaattacg ataatggtac tatggcagta ccagcagtac caggtgctgg
     4201 aaaaacattt ataataacaa atttagtagc taaactatta cttgaacaga aacataagag
     4261 tggtaaaata ttaattctta catatatgaa tagtgctgta aataatttta aaggtagaat
     4321 aagaaagatt cttgaagaga ataaaataga tgatactaat tcgtatgaag taatgactat
     4381 acacagtttg gctgtaaaaa taataaagga aaagccagaa gttgtaatgt taagtgagga
     4441 ttttaacata gctgacgatt tgcaaaaaag tattatatta aatgaatgta taagtagatt
     4501 tagatttgaa ggtggagaaa gagcttttag atggttttta aaggagcaaa aagatgaacg
     4561 ctggaaagaa ataactcttg aagcctggga aagaggtttt tttgaatttg caggaaatgc
     4621 aataagtgaa cttaagtata agggaatttc tccagataaa cttgaagaaa tattgtcaag
     4681 aggacacaaa ggaatgctta aaattatttt accaatatat aaattgtatg atagaaaatt
     4741 aaagcaaaat ggattgctag attatgatga tatattaatt ttagcagaaa aaactttaag
     4801 cttagacgaa gggcttagaa agaagtttca aactagatac aaatatatat ttgaagatga
     4861 gtgtcaggac tcaaatgaaa ttcaaggtaa tataataaaa attatatcta gcgaaaataa
     4921 aaacctagtt agagttggag atataaatca gagtataaca ggaacatttt catcatcaga
     4981 ccctaaattt tttaaagaat ttataaatag tgcagatttc tgttatagga tggatatgtc
     5041 aaatcgaagt tcaaaagata tacttgattt agcaaacact cttgtcaagt atgttacaaa
     5101 ggaatttaga caagaagaat gtagacatgc tcttgaagat atgcaaataa aaactgtgcc
     5161 aaatggaatg ggatataaag aaaaccctaa tccagaacat tataatataa atgtaaagtg
     5221 gtatgagagt tggaaaattg aaatagagca aactgctaga tacgttaagg gtataaaaaa
     5281 taaatatcca gataaaagta taggtatatt ggttccattt aatgaacaag tgacacaagt
     5341 tgctaaagaa cttagagaga ataatctaac ctttgaagaa ttaggaccaa attctttgaa
     5401 taaaaggaag gttataaaca acatagcata tattattgat tttatgttac attgtgatga
     5461 tgtagaaaag ttaatagttg tacttaataa agtttttata gatactgata atgaagaaga
     5521 aataaagtat tttttagaag aattaaaaaa ctattctacc gaagaaatta tttataattt
     5581 tgaatttagt aagttagata atgatttaat agaatttaaa gaagaaagtg atatatactt
     5641 aagttttaaa agaggaatag atattataaa acagatatta gaatatccaa taataaggtt
     5701 agacctctta gttttgttta ttgggaaaaa acttagttta gaaaaagaag ataaagctgt
     5761 actggattat atatcttttt atataaagta tctagtatca gaaaatataa atatggattt
     5821 agaagatgta tataatttat tgtttgatgt aaagaacagg gtgtttaatc atataataga
     5881 tgtggtatat gagataaatg gatatgaacc agagcctggt agtataacac tatgtaacta
     5941 tcataaatca aaggggatgg aatgggattg tgtgttttta ctaggtcttg ttgaatataa
     6001 tttccctgac aatattaatc aaaagtttca gagtgataaa tggtatctaa aagaaaaata
     6061 taaaaaccct atggcaatta ttaaatcaga agttgaagct atattaaaag gaagtatttc
     6121 aacagattat gctcataaaa cgaagattga ttctataaat gaaaagatta gacttttata
     6181 tgtagggatt actagagcaa aggaaatgtt agtactttca ggaagtgctt atagagatga
     6241 aagtgatata gggaataaga gaaaagagca aaaaccatgt atatatttaa gcagacttaa
     6301 tcaacatatt attgaaaaaa ggtcaaatta atatataata agttattaaa agattgtttg
     6361 aaatatatct aaaagaggat aaaacaatat gtacagtaag taactactaa aaaataaagt
     6421 taagagagaa gtgatatgag tgaacgataa attaaaatat ttttcttata gtcaaaattc
     6481 aattaacaca tataaatcat gtccattgaa atttaaatat aaatatattg atagaataaa
     6541 ttggaaaaat gatgatgttg gtagtagaga gtattatgaa actttaaaaa caggaagaga
     6601 ttttcatttg atttgtgaaa gatattttag caatatacct cttggaatct attttaatga
     6661 ggatgataaa aatagtaaaa agtttttaaa gtggatggaa aatataaaaa aagttgttcc
     6721 tattgaaaaa ggaaaaacgt atttgccaga atatgaagta aggatgacat taaatgggga
     6781 tataatacag gcaaagtatg atttagttgt aattggaaat aacaatatag aaatatggga
     6841 ttggaaaacc gagtctaaaa agattgacta taaacacgta gaaaatagaa tacaaactat
     6901 tgtttatatg tttttagcaa aagaagtaat acctaaagtt ttaaaaatgg acataaatgt
     6961 taaagatata aatatgagat attatcaacc tgaatttgat gacttaccaa ttaccatttt
     7021 atatgatgaa gaaaaacatg aagcaaatag aaataaaatt caaaattata taagtatgat
     7081 aaaaaacact aattatgaag aacatacata cgaagacaat ctatacaatg atatagaaaa
     7141 ggtatataat aaagaagaac gtatgtgcta tagaaataaa aagcattgca agtattgtga
     7201 atttaataag ttatgtaatg gcaaagaaat agattatagt attttggagg cggaaattta
     7261 tgggacttag gtttgtatta ggtagaagtg gaagtggaaa atccacctat atactagatg
     7321 agattaaaaa agaagctcaa aaaaatgaaa cgacatcaat tatactttta gttccagaac
     7381 agtacacatt tgaggctgaa aatagagtaa gtaaactatt tttaggaaaa gaaaaagata
     7441 agtatctaag agttagggta cttagcttta agacactaag taatatagtt ttttcacaag
     7501 taggaggatt gacagatgta aatataaatt ctagtggaaa agcaatgatg gtatatagag
     7561 ctattgaaga tgtaagtgaa gaattaaatg tattttcaaa atcaaaatcg cagtctggtt
     7621 ttgtaagctc aattacagat atgatatcag aaatgaaaca atacaatata agccctgaaa
     7681 tgttagaaaa tatatctgga gaattggata atgaaacttt aagtttaaaa ttaaaagata
     7741 taagtaaaat atataattct tttgaaggga aattgcatga aaattatgta gatgctcaag
     7801 atatgttaac ttcattggca agtaaaattg aattaagcag ttatttagat ggagcttgtg
     7861 tttatataga tgaatttaca ggttttacac caaatcagta caatgtcata aagagtatat
     7921 taaataagtc taaaagtgtt aatatctctt tgactgtaga tgatatcaac tatattgggt
     7981 acagtaaatc tgatatgttt tccagaacga aatttactta ttcaaaattg actcaattgt
     8041 gcaacgaaga aggcataaaa atattgccac aagtaaattt aaacacaggt gttataaaaa
     8101 gatttgaaaa agttaaggaa ttacagcact tagaaagatt ttataatgct tatccatata
     8161 aaatatattc aaatcaaact gaaaatataa agataaaaga gtttaacaat ttatattcag
     8221 aggtggaaga gatagccaga gaaattgtac atttagttag agataagaat gtcagatata
     8281 gagatataac cattgctaca agagatttaa atagatatga ttttttagta cactctatat
     8341 ttaatgaata taatatacca aactttatag ataagaagag agaggcaaaa agtaatccaa
     8401 tagttatttt aattatttca gctttagaaa tgaagaatag acgatatggg tatgaaacta
     8461 tgtttaggta tttaaaaagt ggacttatag gaattgataa tgatgatata aatttactag
     8521 aaaattatgt gttggctaat gggattaaag gtaaaaaatg gtttgatgaa aaatgggatt
     8581 atagaataac ccaaagtctt tcaggtcaag aaagtgaatt tgaactggaa ttaaaagaga
     8641 aaataaatga aataaaaaat agagtattag agcctatagt aattttacaa gaaaaattaa
     8701 gaggaaaaaa tagagtaaaa gaaatctgta gatatatata tgaattttta ttagatataa
     8761 atatgccaga aactatagaa agtttaataa taaattttaa ggataaaggg gaacttgatg
     8821 ttgcaaatca atactcacaa gtttgggaca tagtagtgga catattagac caaatggtgg
     8881 aacttatggg tgatgaaata atttctttag aaaaatttat aaaattaata accttaggtt
     8941 ttgatgaata tgaattagga ctggttcctc ctagtataga ccaggtactt gtaagtagtg
     9001 tggatagaat gaaaaatcca gacacgaaat atttatacct agttggaact actgatggtg
     9061 tgtttccgct gattacaaag gatagtggaa tattgagtga taatgatagg gaaagtcttg
     9121 gaaacaaagg aatcgaagtt gatattgata gtaagactag gagttttgaa gaacagtttc
     9181 ttgtatataa agctcttact tcgacatcta agaatttaac cataacatat ccaatatcag
     9241 accatgaagg taaaacgctt agaccatcaa taattatatc aagacttaag aagatatttc
     9301 caaatataga aaataaaagt tatttagtgg aagaaaataa aagcactgat aaagatatac
     9361 ttaaaaaaat aacggttaaa tcccctacat ttaatgaact tataaacgta ataaaaaatt
     9421 atgacagtga tggttacaat acagaagaga ttaatagtat ttggttagat atatataggt
     9481 actatttaaa agatgaaatc tacagttcta taacaaaaaa agttataaaa ggtcttagtt
     9541 atactaatca agttcataag attgaagaga aaaaaataag gtcattgtat aaaagtaatt
     9601 ctcttagcat atctaggctt gaaaaatatg ctgaatgtcc atttgcttat tttatacaat
     9661 atggtctaaa agcaaaaaag cgtaaagagt attcatttac accaccagat ttaggaactt
     9721 ttattcataa tattttagat aggttttcaa aagaactttt acaagataat ttaacttgga
     9781 gagatattga tgaaaaatat atagaattaa agataggtat aatagttgat gaaataattt
     9841 taaagatacc aggatatata ttaaatagtt ctgagaggta taaatacctt gcatatagat
     9901 taaagaatat gttgacaact gctataacta taattagtca gcaaataaaa caaggttcat
     9961 ttgaaccaat agattatgaa gttaaatttg gcaacaatgg aaaatatcca cctataaaaa
    10021 tggtgttaga aaatggacaa gaagtaagtt taattggaca aatagataga gttgatgaat
    10081 ttgaagaagg agaaaataaa tatataagaa taatagatta taagtcaggc aataagtcca
    10141 taagtctaac agaaatatat tatggtctac aattacaatt acttgtgtat ttagatgcaa
    10201 tccttgaaag tgcaaaggat gaagatataa atataaatcc agctgcaata ctatattgta
    10261 gaataaataa tccaatagca aaatttaatg aagataaaga tgatgaagaa atacaagaag
    10321 caatattaaa agaacttaga atgaaggggc ttgtagtaaa agactctcac atagttaaag
    10381 agatggataa atcattaata gatggagaaa ggaaaaactc tttagtaata ccagtaggtc
    10441 ttactaagga tggaaatgta ggtaaaagta cttcagcaat aagctatgag gatttcaaac
    10501 tgcttagaaa atatgtaagg catgctataa aggatttatg tgaagaaatg ttaagtggag
    10561 aaatacgtat agctccatat aaacataaag atggaacatc atgtgacttt tgtgattact
    10621 ctgctatatg tcagtttgat tcaactataa aagataacaa gtataaaaat ttaaataata
    10681 agagtaatga agagataata aaaatgatga aaggagatgt taattagtga gttctcctaa
    10741 atggacaaaa gagcaattag aagttataga aagtagagaa tgcaatttat tggttgctgc
    10801 tgctgctggt tcaggaaaaa cagccgttct agttgagcgt ataattcaaa tgataacaag
    10861 tagagaaaat ccaattgata tagataagtt attagttgtt acatttacta atgctgctgc
    10921 gtcagaaatg agagagagga taggggatgc tataggaaaa gccttagatg aaaacccaga
    10981 aaataaacat ttacaaaacc aacttgtttt attaaataaa tctagtatta caacaataca
    11041 ctcattctgt ttggatgtta taaaatcaaa ctttcataga ataaacttag accctaattt
    11101 cagaatagga gaccaaactg aatgtgctat tttgaaacaa gaagcaatag aggaagtatt
    11161 tgaagattta tatgaagaaa gagatgaggg gtttttaaat ttagttgaaa gttatgcaga
    11221 aagaggagga gataaagaag tacaagatat tatcttggga atatattcat ttgcaatggc
    11281 ttctcctgaa cctaaaaaat ggttgattga ctcagcagaa cgatttaata ttgatgaaaa
    11341 ttttgatttt tcacagtcta tttgggctag agctatatta gatactgtaa aaatagagat
    11401 taatggtctt tgtctaaata tggaaagggc attaaaagaa gtggaatcaa tagaagaatt
    11461 agaaactttt gcagaaaagt tatccgttga atataaaaaa attgctgaca tttcacaagc
    11521 atgtaataaa tcttgggatg aggcatataa aaagatggca agtatgtcct ttgaaaatta
    11581 tgtaaaagga gtaaaaagaa tttcaaaaga tgctccttcg tatataaaag aatcaaaaga
    11641 aaaggcaaaa actataagag ataagactaa gaagtcttta gaaagtatag taagtgctac
    11701 attcaataaa gataatgatt ctattagaga agaaataaaa tatctttata acatagttaa
    11761 accaatatct agtgttgtac tgagatttga agaggaatat agcaataaga aaagagaaaa
    11821 aggaataata gattttaacg atatagagca ttttgcatta aatatactta cagatgtaga
    11881 tgaaaaaggt aatattgtac cttctgatat agcagttgga tatagaaaca agttttatga
    11941 aatatttata gatgaatatc aggatagtaa tctagtgcag gaagtactgc ttaaagctgt
    12001 tgcaaataca gaaactccaa atagatttat ggttggtgat gtaaaacaga gtatatatag
    12061 atttagacaa gcaaagccag aattattttt gcaaaaatat aataactata atgataaaaa
    12121 gggaagctcc catagaaaaa taatgcttta caaaaacttt agaagtagag aagaagttgt
    12181 agatgctgta aattatatat ttgaaaatat aatgaatgag aatataggag aaattgaata
    12241 tacagaaaaa gaaagactaa atttaggtgc aaattttaat gtggatacag acgagaaatc
    12301 tattattggt ggagcaacgg agatacacct aatacaaaaa gataataaac ttgatgatga
    12361 tattataaat gataaagacg atagaattaa taacaaagaa aatgaaatag aggaagaaga
    12421 aaaattagat aatatacaac ttgaggctag aatggttgga aacattatca aagatttaat
    12481 gaaagtcaat gaagatggaa aaattcaaaa ggtctatgat aagggaatag atggatatag
    12541 acctgtggaa tttagagata tagttattct tttaagagct acatctgcat gggctccagt
    12601 gtttgcagat gaattgatga atatggatat accaacgtat gctgatgttg gagtggggta
    12661 ttttgatacg attgaaataa aaacaatatt gtcattactt caaataatag ataatccaat
    12721 gcaagatata cctcttatat ctgtattaaa atcacctata tttggattta caccagagga
    12781 tttgattgat attagagttc agagtaaaga taaaattttt tatgaggttt taaaaagtac
    12841 agcagagtat gatggattta cagattctca aaatgaaaat gagtctgagt ttataccaag
    12901 tgaagaatgc ataaacaaaa gtaaagattt tttaattaag ctaaaggaat ttaaagaaaa
    12961 gtcaatgtac atgagtacag atgaatttat atggtacttg tacacaagga ctggatacta
    13021 tgcctatgtt ggagcattgc caggtggaag tcaaagacaa gctaatttaa aagtattgtt
    13081 tgagagagca aaacaatttg aagaaaccag tcttaaagga atattcaact ttgtaaattt
    13141 tatagagaaa ttaaaaaaat ctagttctga tatgggaagt gcaaaaacac ttggtgagaa
    13201 tgcaaatgtt gttagaataa tgagtattca taaaagtaaa ggtctagaat ttccagtagt
    13261 catatgctct gcaatgggta aaaatttcaa tactcaagat tttaaaaaga gtattttata
    13321 tcatcataat ttagggtatg gacctcaatt tgtagactat gagagaagga tttcttttcc
    13381 aagtatagcc aaagaagcat taaaaagcaa aataaatata gaaaatttat cagaagagat
    13441 gagagtttta tatgttgcat ttacaagagc taaagaaaaa ttgattataa ctggttctac
    13501 tagaaatata caagatagta taaagagatg gtcaaatgga attgaaagtt tagatacaat
    13561 atcacaatac gagatattaa aaggtaaaaa ttttttagat tggataatgc cttgtgtatt
    13621 aagacatagg gatttatcta atctactaga agaagtagga ttagacgcag tttttaatgt
    13681 tgaacataat tcaaaatggt atggaaagtt gtggaataag agtgatattt tagtggaaaa
    13741 gaagagtgat gaagaaaaag aaagtattga agaaattctt gaaaaaatag atgtagataa
    13801 tcctgatagt gattattaca gtgaaataga agaaaaatta aactatatat atccatatga
    13861 atttagtaca agaaaacctg ctactatatc tgttacagaa ataaaaaaga tacaaaataa
    13921 ttatgaagaa gagttaataa atactatatt tgaacaaaaa gtaatattaa aaaagcctct
    13981 atttattcaa aatgaagaag aaagagaaaa aataagtgga acagaaagag gtacaatagt
    14041 ccatttagtt atggaggttt tagacttaaa aaatgtaagt agtgtaaatg atataaaatc
    14101 acaaataaga ggttttgttt caaaaggtat aataacagaa aaacaagcta gtatagtaaa
    14161 tccatataaa atatataaat tttttgcctc taacatagga aaaagaatgt tgaatgctga
    14221 aattataaat agagaaaaat caatttatgc acaagtaaat atgaaagaca tatatattta
    14281 tgaaaagctt ataaacaatg acgataaaaa actttatgat aatgaaagtg tcatgttaag
    14341 aggtatagtt gatgcttatt ttgaagaaga caatcaaata gttttggtgg attataaaac
    14401 agattttgta aatgaggaaa atataaatca aataatagag aaatataaaa aacagttgga
    14461 tttatatgca gatattattg aaactttaac tggtaagtca gtaaaagaga aatgtatata
    14521 tttatttgga gttgatgaag ctgtttgtta ttagttttta tatataaatt aaacctaaaa
    14581 gatagaggaa ggggaaaaaa tgagatttat tcatacatca gattggcatc tagggaagtc
    14641 cctagaaggc cattctagaa ttgaggaaca agcaaagttt tgtgaggaat ttataaaaat
    14701 agtagaatca aatgaaatag atatggttat aattgctgga gatgtatatg atacttctaa
    14761 tccaccagca caagcagaaa aactatttta tcagactgta tcaagacttg ccaataatgg
    14821 tcagaggtgt gtattaataa tttcaggaaa tcatgataat ccagagagac tctctgcaat
    14881 aacacctcta gcacatgaac aggggatact tatatatggt tatccattaa gtgctacgat
    14941 agaagctaag tataaagggt ttgaaataac atatgcaact cagggatgta caaaactgaa
    15001 tataaatgga gaaaatattg tggttgctac attaccttat ccaagtgaaa aaagattaaa
    15061 tgaagttttt tcaagtgaag atgagtttga gaaacaaaaa aattattctg aaaaggtagg
    15121 agatatattt agaagtttag aagaaaattt tagaagtgat actataaata tagccgtatc
    15181 acatatattt gtaataggtg gagagagtac tgaatcagaa agacctatac aattaggtgg
    15241 aagtttttta gttgaaagaa aagatttgcc agaaaaggct caatatactg cactaggtca
    15301 tttacataaa caacaaaaag cttccgaacg tttaaatgca tactattctg gttctccact
    15361 tcaatatagc aaggatgaaa gagcgtatac caaaggggca tacatagtgg atataaaagc
    15421 tggtgaaaaa ccaataattg aagatgtata ttttaataat tacaaaccta tagaagtatt
    15481 taaatgtaat ggaatagaag aagcactaga tatatgtgag caaaatcaag atagagagat
    15541 ttggtcatat tttgaaataa atactgatga gataatatca cagaatgaaa ttaaaaaaat
    15601 gaaggaacta ttaaaagaca taatagaaat aaaaccaata ataacatctt gttatgaaca
    15661 agaaagtgta gatataaaag aaaagtctat ggcagagttg tttagagagt tctattcttt
    15721 tagtaaaggt gtagagccaa aaggagaact tatggattta tttctagata taataagtga
    15781 agaaggtgaa tcagcagatg agaccaatta gattagaact tacagggctt aatagctata
    15841 ttgataaaca agttatagac tttgaaaaat taattgaaag aggtttgttt gggatatttg
    15901 gaactactgg aagtggaaaa tctacaatct tagatgcaat aactattgct atgtatggaa
    15961 atatatccag aaatacaaaa gaatatataa acagtgtgtg cgataaggca attatatcat
    16021 acgagtttga gataggaagc aaaaacacta gaagaagata cattgtagat agaaccattg
    16081 ctagaagcaa aacaggaaca aagacctcta atgctagatt aatagaagta ttgaatgaca
    16141 atacccaaaa tgttttagct gataaagttg tggaagtaaa tgaaaaagta gcgcaggtcg
    16201 taggattaac ggcaaatgat tttactaggt cagtagtttt accacaagac aaatttaatg
    16261 agtttctaag attatcacgt gcagatagaa gagacatgtt agagagaatt tttaatcttg
    16321 agaaatatgg aagaagtctt ggcgaaaaag tgaaaaagag aaaaaatata cagttacaaa
    16381 atttaaaaga tttgaaatca aaattaagtc aatatgatgg aattacagag gaagtatata
    16441 acaatataaa ccaagaatta atagagttaa aaaatttgga aaaagataag aataatgctt
    16501 tagacctagc tcaaaaatct tatgaagaaa gtaaaacagt atatgaagaa caattaaagt
    16561 tagaaaaaaa tgaacttaga aaaaatgagc ttgatttaaa aaatagtgag ataaaagaaa
    16621 agattgctct tgtagaaaat gatagtaacg ctaaaaaggt agacccatac ataagttcag
    16681 ttcaaaatct agaaaaaaag atagatgaag atagcttcgc agtaagtaat ttagaaaaga
    16741 aattagcaat attaaatcag gagcttgaag ttacaaaaaa tagatatgaa aaaataaaca
    16801 aaattaagaa tgaagaaatc cctaaattga gtgaagaaaa aataagattg caacaagcaa
    16861 taaaattaga agaagagtta gttttattag atagagagtt aaaagagcta aaagaaagtg
    16921 gaattaattt aaataagact aaagttgaat tagaaaaagt aaagcaggtg tcagagtcca
    16981 gaaaagatgc agttactaaa tctataaaag aagttgaagg aaagattgat aaagtaaata
    17041 taagtgcaga attaaaacaa aaaatatttc tagcatatga atacgagaaa gattacaaca
    17101 acgtattgga agaaaaaaat caaaagctaa ataaactaga agaaatactc aaagagacag
    17161 aaaatataaa tttaaaagtt agatatattg ataaagataa gaatgatgta aatcgtaatt
    17221 tagaaaattt atcacttcac ttggatgttc ttcttaaaaa atgtcctggt aagagtgctg
    17281 atttactttt aaaatctgaa tatgttactg aattaagaaa taaagcaaat aatactaaag
    17341 aaaatgaaat taagaaaagc agtatacaag atgaattaaa gataatactt gaaagtaagt
    17401 ttaatactga aagggaagta aatttactaa atgaaaaact tgaaaataat agaaagaaca
    17461 gagatgactt agaaaaagaa ttagaagaac taaagtattt aaatttagcc tctgaactta
    17521 gacgtgaatt aaaagaaaat atgccttgtc cagtttgtgg gtcaaaacat catgaaaatc
    17581 atgatataac taagtatgat gaaaatatat cttttgtcaa agaaaaactg gagaaattag
    17641 aaaaagaaaa aataagtata agaaacaata tagaggaatt aaatgctaaa gtaagtgggt
    17701 atttatctat agaaaaaatg aagactaaag aacttgaaga tgtaaaagga aaactagggg
    17761 ggattccatc tagtcaatta ttaaaaaaac ttgatgaaga acaaagaaaa cttgcattgt
    17821 taaaatctaa tattcaggag tgggagaaag aaaaagagag tactgaaaat aaagtaacat
    17881 tagctaaaga agaaaaaaat aaaattgaaa aagaagaatt aaaaataaga gagagtctga
    17941 ataattataa gaaattaaca agagatttaa acatagaaat agaaggttta gaaaataaat
    18001 gtaaaaaact taaacaggaa tacctaggat taaagacgat tactaaagtc tctaatctat
    18061 tgtcaaaagt aaaagaaata agggagaatg aaaaagaatt agaattacta agtactgggc
    18121 attcaaattt attaaaaaat agagactcac tagatattaa aataagagaa aatgagagta
    18181 aactacatga aatagagata gaattaataa aggctagaga attatatgta gaaaagaaat
    18241 tgagtagaga taataagtat aaagaagtta ttagcataac gaaaggtgat ttagctaaaa
    18301 atcttttaca taatgttgaa gaaaatatat gtaagatatt agaacaagaa gaatcaagta
    18361 agaaaaaatt ggaagagcaa agattagaat atgaaaaaaa cattgctgaa aagcacaata
    18421 tagatggtag acttaaaact gctaaggaac aatataaaga ccaaaaatca acactaaata
    18481 aactattggc agaaaataaa tttgaaagta tatatgcagt aaaaagagct ttattagata
    18541 ctgatgcagt aaaaaaattg attgaagaaa tactagagca tgaagaagaa caaaagttat
    18601 tatcatttaa aataaaatca tcaaaagaaa aattaaatgg aagaagcata aagaaggagt
    18661 actttgaaca gttaaaagat gaaatttata atttgaaagt agaaatagga aatatatcca
    18721 aagatattgg agctaatcaa aatcagttaa taacattaaa agactcttta gataagataa
    18781 atgattttaa taaacaactt aaggtagttg aacacaatgt ggacttattg gaagaattag
    18841 ataaatgtgt acaaggaaat aaatttgtag aatatgtttc tacaaaccaa ttaaaatata
    18901 tagcgttgga agcatcaaaa cgattggatg gaataacaaa aggaagatat gcacttgaaa
    18961 ttgactcaac actaaacttt gtaatgagag ataactttaa tggaggagaa agaagaagca
    19021 ttgatactct atctggtgga gagacattct tgacatctct atcattagca cttgctcttt
    19081 catctcaaat acaattaaag ggaagtgcac cattagaatt tttcttttta gatgaaggtt
    19141 ttggttcatt agatagtgaa cttttagaaa ttgttataga atccttggaa agattgcata
    19201 gtaataatct aagtgtgggt ataataagtc atgtcgagga acttaaaaat agagttcctg
    19261 ttaagctatt agtttcatct agtgaggctg gaataggttc aaaagtaaaa attgaatata
    19321 gttagaaaat atatgttaat tgaatatatt atctaatatt tccaaataat atgtaattgt
    19381 gatataataa aaactaatat taaagttatt tataattaat attaatataa aaacatgtaa
    19441 gtaggagtgt ggtaagtgtt ggaatcgccc aataatttga ttcagattat ttttttaata
    19501 gtattactta tagggtctgc atttttctca gcatctgaaa cggccttaat gtcgttgagt
    19561 aaaatcagaa tcagatatat gcaagatgaa ggagttaaag gagctaagtt agtaagttca
    19621 ttaatagaaa atccaaataa gcttttaagt tctatattgg ttggaaataa cgtcgtaaat
    19681 atagcagcaa catccatatc aacatctttg tttataggat taatgggaga aaaaggagtg
    19741 gcacttgcta cagctgttat gacagtatta gtattaatat ttggtgagat tactccaaaa
    19801 acaatagcag caaataattc agaaaaggtt tctctattag tatctaaacc tataaaagca
    19861 attatattta tattaagacc aatagtatgg atatttaata taattacaaa tattatattt
    19921 aaattatttg gtataacaaa taaaggagct aaatcattca taactgaaga agagctaaaa
    19981 actatggtaa atgtaagtca tgaagaaggc gtccttgaaa tggaagagag agaaatcata
    20041 aataatgttt ttgaatttgg agatatgcaa gctaaaaatg caatggtaca aagaatagat
    20101 atggtagcta tagatatgga agatagttat gatgagataa tacaggtatt taaaactgaa
    20161 aaattaagta gaatgcctgt gtatgaagaa actatagatg atattgtagg aatacttaat
    20221 ataaaagata ttatattttt atctgatgaa gaaatagagt catttgatat taaaaattat
    20281 atgagagaac cttttttcac gtatgagttc aaaaagataa cacaacttct tgaagagatg
    20341 aaacttgaaa aaagtcagat ggctatagtt gtagatgagt atggtggaac atctggactt
    20401 ttaactatag aagatttagt agaagtcatt gttggagata tagaggatga atatgatgaa
    20461 gaagaggatg aaatacaagt tataaaagaa gatgagtata ttgtagatgg aagtactaaa
    20521 attggtgatg ttaatgaact tattggtgta aacttggaat ctgaggagtt tgattccata
    20581 ggaggtttta taattggaca cctaagcaga ttgccagaag aaaatgaagt aattgaagtt
    20641 gataatataa gattttgtat agaaagtata gaaaaaaata gaattaaaaa aataagaata
    20701 tatacataat gtaataatgt tcttaaattt attagaagtg tataaatata aaagatatct
    20761 tttatattta tacacttctt tttattttgt attcaaataa aactagtatg aaatggacaa
    20821 aataaaaata taaattagaa gaaaggagat ttttaagatg agcatactag aaaataattt
    20881 tttatacaaa ttaaaaaaca acactatctt tttttgtata tatacattgt tatttatttt
    20941 tatttacaaa gcgtttccat acatagctcc attttttcta ggtgctataa tagcacttat
    21001 gattaatcct atatcacaaa agttagaaaa taagtttcat ataaataaag gtatctctac
    21061 tcttgttcta agttttcttg cagtggctat agtcagtaca gttactacaa taatagtaat
    21121 aaattcaatg aaagaattaa tgggattttt aaataatata tctgctaatc ctgaggatat
    21181 aagtaatact attatgtatt ttttaaataa aataaatgac tttatgaaat catttcaaga
    21241 aatagccaat tttgatgtag aacaattagt aaacaaattc agtggtgaag taatgcaaat
    21301 tacaaaaaat cttcttacta gtatacttgg attggctact tctattccat atattataat
    21361 atttataatt accttgttta ttgctacgta ttttatagca aaagaccttg ataagattga
    21421 aaatagtttt tataatatgt ttactgttga tgttagaaag aaggtaaaaa acgtaaagaa
    21481 agaggcaggg ctttcacttg ttggatatat aagggcctat acaatactta tggctataac
    21541 attttttgca atctggggaa gttttgcttt atttggattg aaatacggtc taatagtagg
    21601 gtttgtagga gctttaatgg atttaattcc attcctaggt ataataacaa tatatcttcc
    21661 agtaattgtt tattattttc ttataaaaaa ttattttata gctataagta tgactgtgat
    21721 attttttgtt ttgtctttga ttagagaaat acttgaacct aaactagtat cagtaaatgt
    21781 tggactaaat ccattagcta ccctagcagc gatttttatt ggaatccaag taaaaggaat
    21841 aattggagta attttttgtt tgggtttggt atgtatgcat gatatactta agaaggtgga
    21901 tattttttaa ttataataat tgatagattt tttaaaaata aaattttatt cacaggtaaa
    21961 taaaaaaata ttaatcttat taagaaagag ggtatatacg tgaaacaaaa agtagtggaa
    22021 agatttttaa aatatgtatc atttgataca acatctaatt ctcagtgtga gaactgtcca
    22081 tcttcagaag gtcaaagagt tttagctaag tatatagttg aagagctaaa aacaatgggt
    22141 gttgatgatg tgagtttaga tgaaaatagt tatataatgg ctactttaaa aggaaatact
    22201 gatggtgtgg atactatagg attcatatca catttagata caattgaaga tgttagtgga
    22261 aaagatatta aaccaaaaat aatagaaaat tatgacggaa aagacatagt tttaaatgaa
    22321 gcgttaaatg ttattacgta tgtaaaggat tctccagagt tggaagaatt taaaggtgac
    22381 gatttaatag tcactgatgg aacaactcta cttgggtctg atgataaagc tggtatagct
    22441 gagatagtga ctgcaataga gtatctaata aatcatccag aaattaaaca tggagatata
    22501 aaaataggat ttacaccaga tgaagaaatt ggtagaggtg ctgatttatt tgatgttgaa
    22561 aaatttggag caaaatatgc atatacttta gatggaggaa tagttggaga acttgaatgt
    22621 gaaaatttta atgctgcaaa tgcaactatt actatacatg gtagaaatgt tcatccaggt
    22681 tcagctaaaa ataaaatggt aaatgctata catatagctg ctgaaatatc agaaatgttc
    22741 ccagcagatg aaagaccaga aacaacagaa ggatatgaag gattttggca tttaaattct
    22801 ataggtggaa atgtcgagaa tgtaagtatg gcttatatta ttagagacca ttgtaaagaa
    22861 aaatttgaaa atagaaaatc tattatgata gagaatatag aaaaaataaa taagaaatac
    22921 gataatagag tggaattgga tttaaaagac tcttattaca atatgaaaga aaagattgag
    22981 ccagttatgt ttatagttga tatagctaaa gaagctatgg aggaattagg tataaaacca
    23041 cgtttagttc ctgtaagagg tggtactgat ggagcaagat tatcttttaa tggattacct
    23101 tgtccaaata tatttactgg tggattaaac ttccatggga aaaatgagtg tataccagtt
    23161 agttctatgg agaaagctac taaactaatt gtaagaatag ctgaaaaata tgctgaaaga
    23221 gtttagttaa aaagtattta aaaattaagc atttaaagtt ataatagcta tatcattctt
    23281 tataagtcaa gctgattata agcttgtgtt gtacttaaaa tggtatagct atttttgtaa
    23341 ttatttttat aaaagataat tatttttagt ttagggtatc aataaaaagt atgaatatca
    23401 ataggtatta acatttaaaa gggggaattg tattgaaaat taaatctaaa aaaataatag
    23461 catttgcact ttcacttggg attatgttgc caagttgtgt atatgcagac aatattagta
    23521 aaaatcaaaa tgcacaggta gatatgaaca attatcttca aggtagaaaa tctccaaagg
    23581 aggaaaaact tattggtgaa gatagatttg atacagcgat aaaaatatct caaagtggtt
    23641 ggaataatgg ttcagaaagg gtgtttctag ttaactcaaa ttcattgcca gatgcattgg
    23701 cttcaactcc tttggcaagt aaattggatg caccaatact gcttacaaat aaaaatagta
    23761 taccatataa tgtaacagag gaaataaaaa ggcttaatcc agcagaagtt attttaattg
    23821 gaagtgaagg agctatttca tcaagtgtaa aagaatctct tgaagatatg gaagtattag
    23881 tcagtagaat cggtggagca gatagagaag aaacatcttt acttcttact agacagctag
    23941 atgatactgg tgatttaggt gtctcaaaag tggcagtggt taatgggtat aatggactgg
    24001 cagatgcaac ttcaatatca agcccagctg caagtgataa tacagctata atatatacag
    24061 gtaaggatag cataagaagt gaggcaaaga attttattac acaaaactca actaagacat
    24121 acatagtagg tggggagtac agtataagta aaaaacttga aggacagtta gtaaactcag
    24181 aaagactagc agggacagat agaaaagata ctaatgcgaa agttttagaa aagttttatg
    24241 gaaaatcaag taaggttaat aatatgtatt ttgcaaaaga tggaagtggt agagaagctg
    24301 atttagttga tgggcttgct gctggagtat tagcatcaaa aacaaagtca cctgtagtac
    24361 ttgcaagtgg aagtttaagt agtgcacaga aaagttttat aaaaaaagta aaggctgata
    24421 aatttgtaca agttggtgga ggtaaaaata gtaaaccata tgcagaagca ttagccttac
    24481 agtaatattg tgtaaatcaa aattaatata taataatagc aagtaaacca agttgcactt
    24541 taatatatat aat
//
LOCUS       DAMKMI010000046        24521 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMKMI010000046 DAMKMI010000000
VERSION     DAMKMI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24521
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.047"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            101..1606
                     /gene="ade"
                     /locus_tag="PC429_001733"
     CDS             101..1606
                     /gene="ade"
                     /locus_tag="PC429_001733"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF3847243.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVPYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAVAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            1958..2422
                     /gene="bcp"
                     /locus_tag="PC429_001734"
     CDS             1958..2422
                     /gene="bcp"
                     /locus_tag="PC429_001734"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF3847244.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            2432..3175
                     /gene="yaaA"
                     /locus_tag="PC429_001735"
     CDS             2432..3175
                     /gene="yaaA"
                     /locus_tag="PC429_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF3847245.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFNLEGYKYKENLSNNSEYIFVKE"
     gene            3303..5960
                     /locus_tag="PC429_001736"
     CDS             3303..5960
                     /locus_tag="PC429_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF3847246.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFAPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      6014..6116
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6226..7491
                     /locus_tag="PC429_001737"
     CDS             6226..7491
                     /locus_tag="PC429_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF3847247.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            7521..8411
                     /gene="metA"
                     /locus_tag="PC429_001738"
     CDS             7521..8411
                     /gene="metA"
                     /locus_tag="PC429_001738"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF3847248.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            8899..9342
                     /locus_tag="PC429_001739"
     CDS             8899..9342
                     /locus_tag="PC429_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3847249.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            9373..11190
                     /gene="ftsH"
                     /locus_tag="PC429_001740"
     CDS             9373..11190
                     /gene="ftsH"
                     /locus_tag="PC429_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF3847250.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            11368..14070
                     /locus_tag="PC429_001741"
     CDS             11368..14070
                     /locus_tag="PC429_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF3847251.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            14104..14802
                     /locus_tag="PC429_001742"
     CDS             14104..14802
                     /locus_tag="PC429_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847252.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKYHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            15159..16115
                     /locus_tag="PC429_001743"
     CDS             15159..16115
                     /locus_tag="PC429_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF3847253.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            16292..16447
                     /locus_tag="PC429_001744"
     CDS             16292..16447
                     /locus_tag="PC429_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847254.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            16577..17590
                     /gene="aroF"
                     /locus_tag="PC429_001745"
     CDS             16577..17590
                     /gene="aroF"
                     /locus_tag="PC429_001745"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF3847255.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            17612..18676
                     /locus_tag="PC429_001746"
     CDS             17612..18676
                     /locus_tag="PC429_001746"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF3847256.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            18676..19989
                     /gene="aroA"
                     /locus_tag="PC429_001747"
     CDS             18676..19989
                     /gene="aroA"
                     /locus_tag="PC429_001747"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF3847257.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            19967..21046
                     /gene="aroC"
                     /locus_tag="PC429_001748"
     CDS             19967..21046
                     /gene="aroC"
                     /locus_tag="PC429_001748"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF3847258.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            21049..22245
                     /gene="pheA"
                     /locus_tag="PC429_001749"
     CDS             21049..22245
                     /gene="pheA"
                     /locus_tag="PC429_001749"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF3847259.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            22292..23101
                     /gene="aroE"
                     /locus_tag="PC429_001750"
     CDS             22292..23101
                     /gene="aroE"
                     /locus_tag="PC429_001750"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF3847260.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            23113..23646
                     /locus_tag="PC429_001751"
     CDS             23113..23646
                     /locus_tag="PC429_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF3847261.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDINMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            23667..24506
                     /locus_tag="PC429_001752"
     CDS             23667..24506
                     /locus_tag="PC429_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF3847262.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
BASE COUNT         9789 a         2642 c         4417 g         7673 t
ORIGIN      
        1 gcctgtggag aattagtaag acatatttta gtatgcaaaa ttctgtgaag caggaaccct
       61 gtgactttag tcatgggagg ttcagtgtag caataaatat atatcacctg gctttattga
      121 tgcacatatg catattgaat ctacaatggt aatgccaatt gaattatcga aaaaattatt
      181 aaaatcaggt acgacaacta tcatagccga cccacatgag ttggtcaatg taaaaggagt
      241 tagtgcaata gattttttat tagaatcgac aaaagatata ccacttaatg tatatattat
      301 ggtgccttca tccgtacctg caacaagttt tgaaactaat ggagttggta aatttagtgc
      361 aaaagacatg gagagttatg taaataatcc tagaatttta ggtttaggag aggtaatgtg
      421 ttttaatgat gttataaatt ctgaaaatga aatcttggat aaactagagc tttttaaaaa
      481 taaagttgta gatggacatg cacctaatat aaatggtaaa tcacttcaga catatgtatg
      541 tgcaggaata gaaaatgacc atgaatgtat aacttttgat gaagtatatg aaaaattgag
      601 agcaggatta aaaattttaa taagagaagg aagcgcagca aaaaatttaa agtctatagt
      661 aagtggtatg ctaaagcata acttgccaat tgaagaattt atgttttgca cagatgataa
      721 gcacttagat gatatagaaa aacaaggtca cataagatgg aatataaaat gtgcgattga
      781 tttaggtatg gaacctgtta gggctataaa agttgcaact tataattctg caaaagcata
      841 tgggttaagg aaaattggag caataggtgc gggttataag gcagatattg ttgtattgaa
      901 tgatttagac aaaatggaag tggatagtgt atataaagat ggtaatttgg taaacgaaga
      961 gatgttttct aattacaatt atgagattaa agataaagaa ttgcttaata cagtcaagtt
     1021 taaatatatt aataaagaaa aaatacagtt aaaagtaagt gaaaaaaatt atgtaatgga
     1081 gattgtacct tatcaaattt tgacaaataa ggtatatgaa tctttaccat gtgcagatgg
     1141 atattttgtt ccaaataaag agtattcaaa actttgtgtt gttgagagac atagaatgac
     1201 tggaaatgta gcagttgctc cactaaaagg atttggcata aaaaatggag caattgctac
     1261 tactgtggcc catgattctc ataatattat agttgcaggg gataatgatg atgatattct
     1321 agtagctata aattacttaa aggaaataca aggtggttat gtaattgtat caaatggaaa
     1381 agttcttgca cacctaagtc ttcaagtagc tggattaatt agtactttta cagctgaaga
     1441 agtacaagaa attactgata atatgttgga gatagcaaga aaaatgggag ttccagagta
     1501 tgtagaccct tttattacac tttcttttat ggcacttcca gttgttccac aaataagact
     1561 tacagattta ggtctatttg atgttgagga atttaaattt atatagataa tataaaagta
     1621 ataataaatg aggctttctc aaaataggga taagtctctt tttattgttg aaatgataaa
     1681 aatatatttg atttcataca aaaaagagca tctcatgaac taaaaatttt atttttagat
     1741 atccttattt taattaatct atcatttaac cttgttttaa gcatagatat cttaataaaa
     1801 ctattttcag tctattttaa catataaata catgaaaatt atatatttat gaaactttat
     1861 acataaaaaa taaaatattt tatttatata tagatttata gggtaataat ataagcatag
     1921 tataaaatta ttaataaaaa caaaaggaga gatatttatg ttgagtatag gaacaaaagc
     1981 accagaattt acactagaag ataaagatgg aaataaggta agtatgtcag attttaaagg
     2041 caaaaaagta gtagtatatt tttatcctaa agataataca ccaggatgta cacgtcaagc
     2101 ttgtgctttt agaaatgcat atgatggatt taaaaaggaa gatatacaag ttattgggat
     2161 aagtaaagat agtataaaat cacatcaaaa atttgcagaa aagcatgaac ttccttttat
     2221 tttactttct gaccctgact tagttgctat aaaagctttt gatgtatgga aagagaaaaa
     2281 aatgtatgga aaaactgcat tgggagttgt acgtgcgaca tatattatag atgaaaatgg
     2341 aattattgaa aaagtctttg aaaaagcaaa accagacaca aatgcacaag agattcttga
     2401 atatttggaa aaacaggagt aataagaatt tatgattata atgttatcac cagctaaaaa
     2461 tatgaagaat atagaagtat ttgatagaga tttgagtttg ccttgcttta tagataacac
     2521 aaaagaaata gtggaaaata taaagacatt tggtatagaa gattttaaaa ataaaatgaa
     2581 aattaatgaa aaattagctg tattaaataa aaatagattt gaaagcatta aatttgatag
     2641 attaggaaac cctgcaatct taacttatga tggaatacaa tataaaaata tagaagcaga
     2701 aaattttaca aggaaagatg aagagtttgc aaatagctgt attcgaatca taagtggatt
     2761 gtatggtgtt gtaaaaccat atgatagtat atatgaatat agattagaaa tgcagacaaa
     2821 attacgagta ggtgagttta agaatttata cgaatattgg ggaaatcgta tatataaaga
     2881 actaatcaaa gaaaaaacag ctatcgttaa tctttcatca aatgaataca gcaaatccat
     2941 agaaaaattt attaaagata gtgatacata tattacatgt acttttaaag taaataaaaa
     3001 tggcatatta aaagtggaat ctacacaagc aaagaaagct agaggtatga tgacaaaata
     3061 cattgtaaaa aataggataa gagatattga agaactgaaa aagtttaatt tagaaggtta
     3121 taagtataaa gaaaacttat ctaacaatag tgaatacata tttgtaaaag agtagaagtt
     3181 ttgttaaata ttaaatggca ttgttaaatt taggtaaaat atacttagtg tgtattgaat
     3241 tgaacttgat tttaaaatat aattattctt aaattaaaaa aagaaaggag tggtcttcca
     3301 aaatggaaaa tcacaaacag atgccttggc attcaagagc aacgcatgaa attttagaaa
     3361 gtctagattc atcagaggag gggctcaccg ataccgaggc ggaaaaacgg cttaaagaga
     3421 atggcttaaa tgaacttaga aaaaaaccac ctagaacaac tttacaaatg ctttggagtc
     3481 aaattacaga tgctatggtt atgatactaa taggagcagc aatattatct ttgatatttg
     3541 gcgaatttac agaagctttt gtaatactaa taattgtaac agtaaatgca gttattggaa
     3601 ttgttcaaga gaaaaaagca gaatcatcat tagaagcatt aaaaaatatg aactcaccta
     3661 cagctcgtgt tatgcgtgat ggtgaagaaa atgttattcc agcaagtaat cttgtagtag
     3721 gagatattgt ttttttagaa gatggagcta tggttccagc agaccttcgt ttaatagaaa
     3781 catcaagttt aaaaattcaa gaggcttctt taacaggaga atctgttcca tcagaaaaag
     3841 atgcagatac aatcttacct aaagaatgtg tattaggaga ccgtagcaat atggcgtata
     3901 catcttcaat agtcacatat ggtagaggtg taggtgttgt agtagcaact ggtatggata
     3961 cagaagtggg taacatagca catcttttag attcacaaga tgattttgat actcctataa
     4021 aacgtaaact taatactgtt ggaaagacat taagtgtcat aggtattatt gtatgcgttg
     4081 ttatatttgc aataggtgca ttttatcaga gacctctctt gccaatgttt atgattgcaa
     4141 tttcccttgc catatcaatt attccagagg gattacctgc aacagctact attgtaatgg
     4201 ctttaggcgt acagcgtatg gctaaaagaa atgctttaat tagaaaatta cctgctgttg
     4261 aaacattagg aggagctaca gtaatatgtt gtgataaaac tggtacgtta accttaaata
     4321 aaatgactgt tacacatatt gctgtcaatg gagattttga atctggaaaa gtgacatcaa
     4381 tagatactgc atctgaaaaa catccattag tgtataaaga gattgtttat gctggtgcac
     4441 tttgtaatga tgcatgtatt aatccagata aaacagatga aatattaggt gacccaacag
     4501 agggcgcatt aatttttatg gccaaaaaat ttggaataga tcaagaagct cttgaagaag
     4561 aatatccacg tgtatttgaa cagccttttg attcagatag aaaatgtatg acaactgttc
     4621 ataatataga tgaaaagatt attgcttata ctaaaggagc agtagacgaa attcttgacc
     4681 tatgtactaa aatattaact tcaaaaggtg aaagaaacat taccgaagtg gataaaaaga
     4741 atatacacga attatgtctt aacatgtcta aagatgcatt gcgtgtactt ggttttgcta
     4801 agcgtgagat ttcatctata ccaaaagaag atagtgaaaa tatagaatat gatttaacat
     4861 ttataggtat ggttggtatg attgacccac caagaactga ggtaattgat gcagtaagta
     4921 cttgtaaaga agcaggtata agaaccataa tgataacagg tgaccataaa gttactgcca
     4981 caacaatcgc tcatgagtta ggaatatgga gtgaagaaaa tactgtgata tctggtgatg
     5041 aacttgataa tttgagtgat gatgaactag atgaagctgt taaaaatact acagtatttg
     5101 ctcgtgtatc cccatttgat aaacttagaa ttatacaatc tctaaaaaga attggagaag
     5161 taccagcaat gactggggat ggtgtaaatg actctcctgc cttaaagtct gctgatattg
     5221 gtatagccat gggtataagt ggtacagatg ttgcaaaaga ttcttctgac atgattttaa
     5281 tggacgatag ttttacaaca attgcttatg caataaaaga aggtagacgt gtatatagaa
     5341 acatacaaaa agttatacaa tttttgcttg ttggtaatat agcagagata cttacgttgt
     5401 ttgtagcaat attattaaat tgggatactc cgcttcttgc tgttcatatt ctatgggtaa
     5461 accttgcaac tgctacgctt ccagcccttg ctcttggagt tgacccacca agcagaaata
     5521 taatgaagca ccagccagtg aaatcaacca ctttgtttga aaaaaatctt atacaaagag
     5581 ttgttaccca aggtatattt gtggcaataa tgactatttt agcatataaa attggtctta
     5641 atatagaaaa tcattctgta ggtcaaacaa tggcattttg tgtattagca ttttcacaga
     5701 tgttacgagc atttagtcag cgttcaaata cagattcaat gtttaataga aataatggta
     5761 aaaatccatt tcttttaatc tcatttttaa cttctgcatt tttaatagca gtaatactat
     5821 ttgcacctgt ttttagaaat gcttttaact taacagtttt aaataactta gagtggctta
     5881 taacgattgt tttagccata atgtctgtaa ttcaagtaga ggttgggaaa attattaaaa
     5941 gaaaaaaatt aaaaaaatag ttgacaaaat taggaaaaat atataagata taaaacatag
     6001 aaaaattaaa taaatcttat ccagagaggt ggagggactg gccctatgaa acccggcaac
     6061 cagtatattt atatacagtg gtgctaaatc ctgcggcgaa agctgagaga tgagtataat
     6121 taagcttata cagagcctga gttatatact caggctttat ttattttaac tctcatctgg
     6181 taaatagttt aatttttttt actaaaatct aagggggata caagaatgta taataaagaa
     6241 acaatatgtg tgcaaggaaa ttataaacca ggtaatggag aaccaagagt actaccttta
     6301 tatcaaagta caacttttaa atatagcagt atagaccaac ttgctgaatt atttgattta
     6361 aaagttgatg gacatatata ttcaagaata agcaatccta ctattcaagc ttttgaagaa
     6421 aaaataagtt tactagaggg tggagtatct tctgtagctg tatcatcagg gcaatctgca
     6481 aatatgttgg cagttttaaa tatatgtaaa tcaggagata gtatactttg ttcttcaaaa
     6541 gtatatggag gaacattcaa tttactagga cctagtctta aaaaatttgg tatagattta
     6601 atatcgtttg acttagattc aagtgaagat gagatagtag aacttgcaaa ggaaaatact
     6661 aaggttgtgt ttgcagaaac acttgcaaat ccaactcttg aagtcataga ttttgaaaaa
     6721 atagcaaatg tagctaagag aattaatgtt ccatttattg ttgataattc attagcatct
     6781 ccagtgcttt gtaacccttt aaagtatgga gcaaatatag ttactcattc taccacaaaa
     6841 tatttagatg ggcatgcttc aagtgttgga ggaattatag tggatggtgg aaactttaac
     6901 tgggataatg gaaaatttcc agaattagtt gagccagacc caacatatca tggtataagc
     6961 tatactcaaa aatttggaaa tgccgcatat gcaactaaag caagagttca gttgcttaga
     7021 gactatggaa attgtttaag cccattcaat gcgtatctta ctaatttaaa tgttgaaaca
     7081 ctacatctta gaatggagag acatagtgaa aatgcactta aaatagctag atttttagaa
     7141 aaacatgaaa atgtagattg gattaattac ccaggacttg aagataacaa gtattatgag
     7201 aatgccaaaa agtatttatc aagaggatgt agtggtgttt tatcatttgg agtaagaggt
     7261 gggttagaaa atgccaaaaa atttgtggaa aaattacaga tagcatcttt ggttacacat
     7321 gtttcagatg taagaacttg tgttatacat ccagcttcaa ctactcatag acaattaaca
     7381 gaggaacaat taattgcatc tggagtattg ccttcactaa taagattatc tgttggaata
     7441 gaaaatgtag aggatttaat agctgattta aatcaagctt taaatttcta atattctaag
     7501 agaaagagaa gtgaaaagat atggctttaa tactaccaaa gggattacct gttattaata
     7561 aattattaga tgaaggaata gatgtaattt ataaagaaga ttttaaaaag gaattaaagt
     7621 atgaagaaaa catagacact aagatagcta tactaaatct aatgcctata aagatagata
     7681 cagaattaga tttactaaga aggatagata aaacaggatt caacgtgtct gtggaattta
     7741 ttaaaatttc aacaagagaa agtaaaagaa gttgtaatga atatgttaaa gaattttata
     7801 aaacatttga tgaagccaaa ggagagtatt ttgatggctt tataataaca ggagctccag
     7861 ttgaacaaat ggagtttgaa gaagttgatt attggaatga actagaagaa attatggatt
     7921 attctaagag aaaaactaaa tctacattat atatatgttg ggcagctcaa gcaagtcttt
     7981 acaaatacta caatgtaaaa aaacttccac taagtcaaaa atgttttgga gtatttaagc
     8041 ataaggtaga taaagatagt aaaatagtag atggatttga aaatgaattt tttgctccac
     8101 attcaagaca tactactgta aatattgaag ctttgaaaga gaataaagag ttgagcattg
     8161 tgagtcattc taaagaagct ggtccctata taattacaaa ttcaagagat gtgttcgtaa
     8221 tgggtcatag cgagtatgac aaatacactc ttgataaaga atataagagg gatataaata
     8281 aaggagataa aatatctata cctcaaaatt actatattaa tgatgaccct tcagaagagc
     8341 caactgtaaa atggaaaaaa cattcagaat tgttgtttag aaattggata aaaaactatt
     8401 tgatacaata aattttaaga gtatataaat gttaactaaa aaaatataga aatttatagg
     8461 tataaagtta atcttaatga tactttatac cttatttttt taaaatttga ccttataata
     8521 gtagttttat attttaagaa aatcacttta ggtgtatgta gatagaggtg gctttaattt
     8581 tatatatggt gcgataatgg gagactaaac ctttatagta tcaataaatt tgtattttaa
     8641 cttttaaaag agattaaaaa gggagaaatc agtgtaaaat atatatagat tttttattaa
     8701 attttattaa aaatatcctg tataaaatta aatatcaata agataacaaa atttcaattg
     8761 aagttacaat attgtaattg acaataaata cttaatacta taatatttaa tacattaaac
     8821 attatagtgt taagtattta ttacagtatg cacttcagcc taaatttgaa ataaagtatt
     8881 ttcgtaggga gcgtttgtat ggatgagatg agttttaaga aagagctact agagttgact
     8941 agagatataa atatgaaatt tacaacttta ttaagtgatt tttatcagcc acttggtatt
     9001 acagcagtgc aagctttgat tttatctgag ttatgtgaac atggagagaa aaaaataagc
     9061 gatttaggga agaacttaaa catgacaaat agtaatgtat cagtaatctg tcaaaggctt
     9121 gaaaaaaatg gctttttaaa tcgcataaga gatatagaag accaacgaat tgtaaaagta
     9181 aaagttacaa ataaatcatt ggatatacaa gaacatattt caagtagtat ttttgatagt
     9241 tattttgaaa atatgaccag tgaaaaattg caagatatgg aagatataat agaaggttta
     9301 gagaaattaa ataaactttt aacttacatt gattgcaaat aacataaaaa taatttaaag
     9361 gagaataaaa ttatggatga acataaaccc caaaaaaggt cagcactcta ttattataca
     9421 gttataataa tagtagtaat gtttttaaat gctttaatat ttccaagttt aatggataaa
     9481 aaaatggttg agattgacta tggaaagttt ctcacaatgt tggatacagg aaaggttaaa
     9541 tctgttgaga tagaaaataa tagaatagca ataaatccat ctagtagcaa ggataagaat
     9601 atatacataa ctggtagaat ggatgaccca gaattggtag atagactaaa aaatgcaaaa
     9661 gtagaattta ctaaggttat tcctaaggaa aattcacctc ttttaagtat tttacttaca
     9721 tggataatac ctataggtat tcttatgcta tttggtaact taatgatgaa gtctatgcaa
     9781 aagcgaatgg gtggtaatgc tatgcaattt ggaaaaagca acgccaaaat gtatgtatct
     9841 gctcaaagtg gaaaaagatt taatgatgta gctggtcaag atgaggcaaa agaagcttta
     9901 actgaaatag tagatttctt acacaatcca gaaaagtata aaaagatagg agcacaaatg
     9961 cctaaaggag cacttttagt aggacctcca ggtacaggaa aaacactcct tgcaaaagct
    10021 gtagcaggtg aagcaaatgt accatttttc tcaatatctg gttctgagtt tgttgagatg
    10081 tttgtaggaa tgggagcttc taggattcgt gatttattta aacaagcaaa agaaaaagct
    10141 ccatgtatag tgtttataga tgagattgat actattggta aaaagcgtga taatggaagt
    10201 ggattaggtg gaaatgatga acgtgaacaa actttaaatc agttgttaag tgagatggat
    10261 ggttttgatg gtggaattgg agtagtaata cttgctgcta caaatagacc tgacagtctt
    10321 gacaaagcat tacttcgtcc aggaagattt gatagacgta taccagttga acttccagac
    10381 cttggaggac gtgaagctat attaaaggta catgctcaaa atgtaaatgt aagctcagat
    10441 atagattata atgcaattgc aaggtctaca tcaggagcat caggtgcaga acttgcaaat
    10501 attgtaaacg aagcagcact tttagcagtt aagtctggca gagatatagt agaacaacag
    10561 gattttgatg aatctgttga aactgtaatt gctgggtatc aacgtaaagg agctgtaatt
    10621 tcagataagg aaaagaaaat tatagcatat catgaaatag gtcatgcact tgttgctgct
    10681 atgcaaaaac attctgcacc tgtccataag attactataa tacctagaac ttcaggagca
    10741 ttgggatata ctatgcaagt tgctgaagat gagagtgtac ttatgtctaa agaagaagca
    10801 attgataaaa taactacttt tacaggtggt cgtgctgctg aagaggttat ctttaacact
    10861 tgtacatcag gagcatcaaa tgatatagaa caagctacta aaattgcaag agctatggtt
    10921 actcgccttg gtatgagtga agagtttgat atggtcgctc ttgaaactgt aagcaatcaa
    10981 tatttaggag gagatacttc tcttgcttgt tcttccgaca ctgctgctaa aatagatgaa
    11041 gaggtattta aaatagtaaa ggcttgtcac aaaaatgcaa ttgaaatctt agaaaataat
    11101 attgataaat tgcatgaact tgctaattat ttattagaaa aagaaactat tacaggtgat
    11161 gagtttatgg aaatacttaa cttaaactaa aatgattata cacaagataa aaatgcctat
    11221 tgttaggcat ttttatttta caactaaatt ttttaaattc atatttaaat ctttacataa
    11281 tcttaatcat taaaaataca tcttaactac aattttatat cggaattgat attataatag
    11341 taatgaatgg tgtaaggagg ttttttaatg gatagaccta atcctgatat tctattagaa
    11401 aaaattaaaa atgaagaaga aaaactaagt agaggtcaat taaaaatatt ctttggttat
    11461 gcagctggag taggaaaaac ctactctatg ttggaatctg cacaaaattt aaaaaaagtg
    11521 ggtgtagatg tagtagttgg atatattgaa ccacacacta gaccagaaac attggcttta
    11581 cttgatggct tggaaattct tcctgtaaag gagatagaat ataaatctat aaaactaaaa
    11641 gaatttgatt tagatgctgc tttagcaaga aaaccagaag ttatattagt agatgaattt
    11701 gcccattcta atgttgctgg gttaaggcat acaaaaagat ggcaagatat agaagaattg
    11761 ctgttagcag gcataaatgt ctacactact gtaaatgtac aacatttaga aagtctaaat
    11821 gacattgttg agataattac aaacgtatca gttaaagaga ctataccaga taagtttttg
    11881 gatacaaata cacagttaga acttatagat gttgaacctg atgtacttct tgaaaggttc
    11941 aatgaaggta agatatataa gaaagaacaa gctactagag caaaaaataa tttttttata
    12001 aaagataacc tggttgcact tagagaaatc gcacttagaa aaactgcaga gagagttaat
    12061 aaagaagttc aaatgaccag actatcaaag ggtgatgtaa ctgtaatacc tacatcagat
    12121 acacttcttg catgtataag tccatctcca tcttcagcaa aagttattag aactgcatcc
    12181 agaatatcag acagtacatt tgcaaaatgg atagctttat atgttgaaac tccaaacaca
    12241 gcaagacttc catttgatga acaaaaacaa ctacaaaata acctaaaatt agctaagaaa
    12301 ttaggtggag aaataattgt tttacatggt gaaaatataa tagagcaaat acttagaatt
    12361 gcaaagctga gaaatgtaac aaaaattgta ataggtagaa atcattcaag taataaaaga
    12421 tttagtaaaa gatttaaaaa agatatagtt gacaaattaa ttgatgaagt tgattatata
    12481 gatatacata taattccata taaatttgcc tcagatgtta aatacaaacc taaaaaggat
    12541 aaatcatcaa taaaatctaa atttaaaatt agcaaagttg attttataaa acttatattt
    12601 attactttga tgataagtat tttagcatat gcagtacaaa gcatgggatt tataagagaa
    12661 aatatattgt taatatatat gttgggtgta gttttagttt ctatgtggac taaagggtat
    12721 agtacaggta taataagttc tgtatttaat atagttcttt taaattattt tttcacagca
    12781 ccattatata cattatctat agcagattca aactatatag taacactggt tgtattctca
    12841 atagtaggta taataactag cactcttacg tctaagatac agcatgaagc agaaactgct
    12901 gctaaaagag aagaaaatac aaaaatgata tatcaaataa ttaagggatt tttaagatta
    12961 tcaaataaag atgatatagt aaacaaaggt atagaacttt tgtctcttag cttaagtaga
    13021 gatgtaattt gttatttaaa tgtagataag cataataaat caaagcttta caaaaaaaat
    13081 actaataata aggataaaaa tgatttggat agtgaagatg aaaaagctgt tgcaagttgg
    13141 gttttatcca attcaacagt agcaggaaat gacacggata cacttcctgg ttcaaaggga
    13201 tactatatac caattgtagg aatgaattta actttaggag tagtgggaat atcatgtatt
    13261 gattcaaaat tggatacaga agatataagc ttaattgaaa caataattgc tcaaatggca
    13321 atagcgcttg atagagaaat cttgtcagaa gctaaagaaa ataccaatct tgagattgaa
    13381 agggaaagat taagaagtaa tcttttaaga gcagtttcgc atgaccttag aactccacta
    13441 gcaggaatat ctggtgcagt aagtacaatt ataaaaaaca agggtactat aggtcaagat
    13501 ataatagatg aattacttag tggtgtatat gaagatactc agtggttaat aagacttgtt
    13561 gaaaatttac ttagtatgac caagatagat gaagggaaat tggaagttaa aaaacattct
    13621 gaactggttg aggaaattat atcagaagcg ttacaaaaaa taaaaaaaag aattgaaaat
    13681 gcacttatag atattaatat accagaacag atattatttg ttcctatgga tgcaaaatta
    13741 atagaacaag ttttaattaa tttaatagat aattctttga aatattcaaa ggaagattgt
    13801 aagatagaga taaatgtata tgagaaagat gattatgttt ggtttgaagt atctgataat
    13861 ggtcctggta tatcaaagga acttaaaaag catatctttg acagattttt tactggagaa
    13921 gaaggagcta aagattccag aaagggtgtt ggacttggat tatcaatttg taaatctata
    13981 attcaagctc ataaaggtga aattatggta gaaaacaata aagataaggg ttcaactttt
    14041 aagtttagtt tgcctaagga aaatgaatag ttttaaattt taaaattagt tttggaggaa
    14101 gaaatgagta aaaaacttat attgttagta gaggatgata agacaataag aaaatttata
    14161 tctactgcac ttttaacaca ggattatgat gtcaaggagg ctataactgg aaaagaaggt
    14221 atatctatag ctgtctcata ttctccagat gttgttcttt tagatttagg cttagaagat
    14281 atggatggaa tagaggtaat aaaagctatc agacaatttt caaatatacc aataattgta
    14341 gtttcagcta gagagcaaga tagagacaag gtagaagtat ttgatgcagg ggcagatgat
    14401 tacttaacaa aaccattctc catagtagag ttattagcaa gagtaagagt agcatttaga
    14461 cattcacaag tagaagctca acaaaaagat gatgttaaat ctacgtttga agtagataaa
    14521 ttattgattg attttgataa aagaaaagtt attgttgatg atgttgaagt ccatttgaca
    14581 cctattgaat ataatattct ttcattactt gcaaagtatc atggaaaagt gcttactcat
    14641 aattttataa taaaagaaat atggggtagt gttataggga atgagactaa gtctttaagg
    14701 gtctttatgg caacgttaag acgaaaaata gaaaaacaac ctgcaaaccc aagatatata
    14761 attacagaag ttggtgtagg atacagatta aatgatgaat agttgtaata tattaattat
    14821 tagattatgt gcttattaat atatattaaa attatatagt ttagttaaaa taagaatgaa
    14881 gtgtatgtgc ttgttgtcag tttagcacat gcattttatt ttttagcttt tttagtatca
    14941 ataatttttt agtatcaata atatgaaata tgttttaaaa taagtatata gtgtaatctg
    15001 taataaaaac tttggttaaa taataaatgt atttaacact taccttataa taaatttaat
    15061 ataaatatta aataataatt attaaaatag tgtaaaataa ttacagtaag gattataaaa
    15121 aatttttaga atgtaaattg aaatttagga gtagatgtat gtatatactg aatttaaata
    15181 caagagagac tattgaagat tttagagata aattttatgt agcagaaaat tcttatctta
    15241 tattaagtgc tccaaaaaat ttaaaattgc ttaaggaaac tttggatata gatgaaataa
    15301 cttttaatga ttgtcttaaa tttgatgaaa taaccaaact tgatttattt gataattacg
    15361 attttttaag cctaaataca tttgaactaa gagatggaga agctgttata gaagaggtaa
    15421 atatgtattt atcagataat tttatattgg ttgtagtgaa tgaagaacac tttttatttg
    15481 aatttgtaaa aaatattatt ttgaagaact ctcaacttga aaaaaatcca gttattaatc
    15541 tatttaagat aaactacttg attcttagag aagttataaa aaatggtttt gagtctttgg
    15601 aaaaagttga agagttaatt cttcaaatag aagatgaaat gatggacaac attaataaga
    15661 atcatgtatc tagaataagt gatgtaagag gattaacaag aattatagtt aagaatacta
    15721 gacctctttt gtatattggg gatagaatag ttaaggaaaa tataagatat ttaaaatatt
    15781 caaatgtaaa aaaatataat ttagaaaatt ttcaaggtat agattttgga attgacaaat
    15841 tatacaattt cgcattgtca acacgagagt tagcagataa attattagat atttattctt
    15901 ccagagtagg tgagaaaaca aataacttaa ttacaaaatt aaccttgtta acagccatat
    15961 cagcaccact tacaattata actggtatat atggaatgaa ctttagatat atgccagaat
    16021 taaactggat ttatggatat ccagcaactt tattttttat gctatgtata atttttgttg
    16081 gaattataat atttaaaata aaaaaattat tgtaaattac agatgatgtt taattgattt
    16141 tataaaaata catcaattga gaagctgatt taagtaaata aaaaaatatt ttaaattgaa
    16201 aatatttata gaaaatattg acaattatat tttctttgtt ataattatta aacaagaaga
    16261 ataataaatt ataaaaatgg aggataatac tatgaaagga atattaaaag aactaacagc
    16321 aaaactttat agcttttttt actttatgtt ctggggcttt tattttagcg ctggctattt
    16381 cttttacaaa aatatatgta cttcctttct ggtgaggcct tattttagtg attttgagca
    16441 taagtaaatt ttataaaaat gattatttta tatatttata taagatttag tgcttgatag
    16501 attattttag agaactcatt agtgagttct cttttttatt ttaaatataa aattataata
    16561 ttgggaggta gtaaaaatga tagtagtatt aaaaatggga gcagataaaa atgaagttaa
    16621 aaaattaatt gaggctattg ggagagaagg agtagaagtt aatcccatag atggaacaga
    16681 attaactgtt ttaggtttag ttggagatac tagtaaaata gatgccaaaa gaatagaagc
    16741 aaataaggta gttgaaaaag ttatgcatgt ggtagagcct tttaaaaagg ccaatagaaa
    16801 atttcatcca gaaccttcaa tcataaatgt taatggaatg gaaattggtt caaagaaaat
    16861 tgctatgata gctgggcctt gttctgttga aacagaagac caaatagtat ctatagctaa
    16921 agatgtgaaa aaatcaggtg caggattttt gagaggtgga gcattcaaac caagaacatc
    16981 accatacgct ttccaaggat tgaaatatga tggattggat ttattaaaaa aagcaaaaga
    17041 aaaaacaggg cttcctatag ttacagaaat aatgtcaaca caagatatag atatatttga
    17101 agaaaatgta gatgtaattc aagttggagc tagaaacatg caaaactttg atttattaaa
    17161 agaattaggt aaaacaaata aaacaatact tttaaaaaga ggattatctg ctacaattga
    17221 agagtggcta atgtcagcag agtatataat ggctggagga aatgaaaatg tagttctttg
    17281 tgaaagaggt ataagaactt ttgaaacata tactagaaat actttggatt taagtgcaat
    17341 tttagctgtg aagaaactta gtcatttacc agttatagtt gacccaagtc atgcagcagg
    17401 taaatcatgg atggttgact ctttatctaa ggcagcaatt gcagttggag cagatggtct
    17461 aataatcgaa gttcataatg acccagcaca tgcactttgt gatggaaaac aatcgattaa
    17521 gccaaatgaa tatgatgaat tgataagtga attaaagact atcgcatcag cagttggtag
    17581 agaaatataa tctataaaag gatggttaaa cgtggaagaa aaactattag taaatttaaa
    17641 agaaaatagc tacaatatat ttattaaaaa aaatatacta aagaatatag gagaagaaat
    17701 aaaaaaaata tattttggaa ataagatttt tattgtaact gatgaaaata tagcaaaatt
    17761 atacttggat aaagtaaaaa ataatttaag tgaatctgga tttgaaatta aatctttaat
    17821 attaaaatct ggtgaagaaa caaaatcatt taatactctt cctaaaatat acgatagttt
    17881 acttgacttt aagttaacta gaaaagattt gattattact ttaggaggag gggtaatagg
    17941 tgatttaggt ggatttgcag cagcaacttt tttaagaggt ataagtttta ttcaaattcc
    18001 aacatcttta ttagcacaag ttgatagttc tgtaggtgga aaagttggag ttgaccttga
    18061 gagaggcaaa aacttagtgg gaagctttta ccaaccaaag gctgtgttta tcgacccaaa
    18121 tgtattatta tcacttccag aaaaattcta tagagatgga atggcagaag ttataaaata
    18181 tggatgtata aaagacaaaa cttttttcaa taaactttta agtttaaaaa atagagaaga
    18241 agtaatgcaa catatagaat acattataca taaatgttgc tttattaaaa aaactgtagt
    18301 tgaagctgat gaaaaagact ttggagatag aatgcttcta aactttggcc atacattagg
    18361 tcatgctatt gagaaatact acaattttac gggttatacc catggagaag ctgttgccat
    18421 aggaatgtat aagataactt tagaaagtga aaagatagga attacaaaaa aagggacatc
    18481 agaacttata aaatcaattt taatgaatta taatttgcct tatgagattg aattaagaga
    18541 taataaatct atagtagatg ctatatctct tgataaaaaa aatctaggtg atacattaaa
    18601 aataatactt ttaaaagata ttggagagag tattatatat aatactactt ctaaattttt
    18661 tcaggaggta agataatggg aagtctaaaa atatatccaa gtaaattatc tggagatgta
    18721 aaaataccac catcaaaaag tatggcacat agggcagtta tttgttcatc tctaagcaat
    18781 ggaaaaagta gaatttctaa tatagatttt tctgatgata taatagctac tataagagca
    18841 atgacatcat taggagctat tatagaaaag aaagaagata ttttagaaat ttcgggaatt
    18901 ttttctaaag aaggtatttt aaatagagag aatcagttga atcaacctaa attgacaata
    18961 gattgtaatg aatcaggctc tacattgaga tttttagtac ctatatcttt agcatttgat
    19021 ggtgtaaaaa ggtttatagg tagaggaaat cttggaaaaa gaccacttga tacttattat
    19081 gagatatttg acagacagaa tataaaatat agttacaaag aaaatcagtt agatttgatt
    19141 atatcaggta agcttaaacc agatgaattt agagtaaaag gaaatataag ttctcagttt
    19201 atcacaggtt tactgtttat attgcctacg ctagagagtg attcaaagat aataattact
    19261 actgagctag aatctaaagg atatttagat ttaactcttt caacgataaa agattttggg
    19321 gtagaaatta taaacaataa ctataaagaa tttattataa aaggaaatca aacgtataaa
    19381 gcgagagatt ataaagtaga aggagattat tctcaaggag cattttattt atcagcagat
    19441 gctataggag aagatattag catattagac cttaaagaag attctcttca aggggatagt
    19501 gaagtagttg aaattttatc taggatggga atggaaatat taagagaagg caacaaaata
    19561 aaaggaataa caaatggttt aaatggtact ttgatagatg catctcaatg cccagatata
    19621 atacctgtat tatctgtagt agcatcctta agtataggga ggactacaat aattaatgct
    19681 ggtaggctta gaattaaaga atgtgataga cttcatgcaa taaatgttga gttaaataaa
    19741 cttggagcaa atatagagga gaaagaggat agtttgataa tcgaaggagt aagcaagtta
    19801 aatggtggag tagaggtgtg gagtcacaaa gaccatagaa ttgctatgac acttgcaatt
    19861 gcttcttgta gatgtgataa accaataata ttaaaagatt ttgagtgtgt atcaaaatct
    19921 tatccacatt tttttaaaga ttttaagatg ttaggaggtc gaattgatga gtggaatatg
    19981 gggaaataat ttaaaagtat caatttttgg agaatctcat ggaaatgcta taggtataaa
    20041 tatagatggt cttccttcgg ggattgaact tgaccttgat aatattgata aagaaatgaa
    20101 gagaagagca cctggaaaaa attctatatc tacttcaaga aatgaaagtg atataccaga
    20161 aattctaagt ggatatttta atggaagaac tacaggaacg ccattatgtg caatcataag
    20221 aaatagtgat actcgttcaa aagattatgg agagcttaaa aacttaatga gaccaggaca
    20281 tgcagatttc acaggtaatg tgagatattc tggatttaat gactatagag gtggaggtca
    20341 tttttcaggt aggataacag ctccattagt attttgtggg gctatatgta aacaaatttt
    20401 atcacaaaaa ggaatagaga taggagcaca cataaaaaaa attaaaaata tagaagatat
    20461 gagttttgat tatgtaaata tttcaaaaca acagttatca aatttgcaaa ctttggaact
    20521 acctttactt gatttatcaa aggaagaagc catgaaaaat acgattatag atgcaaaaaa
    20581 tcaaggagat tcagttggtg gtataattga gtgtgcagta gtaggtatta atgtagggct
    20641 tggaaatcca ttttttgact ctgttgaatc tactttatca catttattgt tttctgttcc
    20701 tgctgtaaaa ggtgtagagt ttggtttagg ttttgaactt gcagatatgt atggctctca
    20761 aagtaatgat gaaatgtatt atgaaggaaa tcaagttaag agcaaaacaa ataacaatgg
    20821 aggaattata ggagggatta ctactggaat gccaataatt ttcaaagtgg caattaagcc
    20881 aactccatca attagcagac aacaaaacac agttaatata aaagataaaa aggacgatat
    20941 tttatatatt aaaggcagac atgacccttg tatagttcaa agggctatac ctgtaataga
    21001 agcagtaaca gccattggaa tttttgattt gatgaaagga cgataataat ggaggattta
    21061 acgagatata gaaatgagat tgacaatata gataaagagc ttattcaatt atttgaaaag
    21121 agaatgaata cagttcttga aattgccaga cataaaatga aaaataacac tactatactt
    21181 caaaaagata gagaagaaaa agtactaagt agagccgtag ataatttaga taataaagca
    21241 tattcacaag aaacggtaca gttttttaat tctataatgg aaattagtag aggattacaa
    21301 aaaagattta tagataatga tggtgagcag aaaatcgagt ttagtttaga ttcatcaaat
    21361 aagaattttg atttgtcaaa tgtaaataaa tataagtctt taaaagatga attaaataaa
    21421 aaaaatatcc tagtaggctt tcctggaaaa tcaggttcat ttacagaaga agcactaaat
    21481 aaattcttca acaaaaaaat atcaaaaaaa caatttaaag aatttgaaga tgttttcata
    21541 gcacttaaaa ataaagaaat aaactatgga ataatcccca tagaaaattc atctacagga
    21601 gctatctctg aaacttatga ccttttgaga aagtacggtt tttatatagt tggagaagaa
    21661 tgtataaaaa tagaccaaaa tttaataggg ataaaagaca caaagcttga agatataaaa
    21721 gaaatatatt ctcatccaca aggtatagga caatgtagtg aatttttaaa acaaaatagt
    21781 acatggaaat tgataccctt taataacaca gcaacaagtg cagaacttgt aagaaaactt
    21841 caagataaaa caaaagcagc aattgcaagc aaaaaagctg caagtattta tggattagaa
    21901 ataataagtc cttgtataaa tgatatagca aacaactata caagatttgt agtaatcagt
    21961 aatcaaatac acattgaaga agaatcaaac aaaatgagtg tagtattttc tgttgaacat
    22021 gaagcaggaa aactttataa agtacttgga tactttgcta aaaataatat aaatatgaca
    22081 aaaatagaat caagacctat gaaaaatgcc tcatggagat atttctttta tatagatttt
    22141 gagtgttcta tatacaatgc taaagtgtac gatttattag aattgataga acataataca
    22201 gcatatttta aatttatggg agtatataaa aataaaactg aatagataag ataaattttt
    22261 atttaattta taattattag gaggtgtaag tatgaatttt ttcggtctag taggtgaaaa
    22321 actctctcac agtgtttcac ctcaaatcca caagagagta ttcgagatat tgaatattga
    22381 aagtgcttat aaaaactttg aaatttcaaa agaagatata tctaagctag acggtgctat
    22441 aaaattatta ggaatacaag gggtaaatgt tactgtacca tataaggagc gtattatgaa
    22501 atatcttgat tttatatcac cagaggcaaa acgtatagga gcagttaata caattttact
    22561 cagagaaaat atgttatatg gttacaatac agattacttt ggtcttgata gtatgtttaa
    22621 aatggctaat attgatgttc aaggaaaagt agcagttata ttaggaactg gaggtgcttc
    22681 taaagctgct cttacatatt ttattgattc tggtatagaa aagctttatg tttcaactag
    22741 aaaaaaggat gataaaaaat tattaaatag taaagctata ttaatagatt atgaagaact
    22801 taaacatata aaaggagata taatattaaa tgctacacca gtaggtatgt accctaatgt
    22861 aggaatttca ccagtgtcaa aatctattat acaaaatttt gatatattaa tagatttaat
    22921 ttacaatcct ggagaaacag agtttttaag aattggtaat agtatgggta aaaaaacttg
    22981 tgatggatta tatatgctgg tggggcaagc aataaaatct caggaaattt ggcaagatac
    23041 taaaatagat aatagtattt tagatgttat atataatgaa ctaaaattag aatttttgta
    23101 agggagattg ttatgataaa taaaacaaaa gaaaaattga tattaatagg tatgccaggt
    23161 agtggaaaaa ctacaattgg taaacttttg gccaaagaat ataattgtag tttctgtgat
    23221 atggatgatt atataattca aatatctcaa aaaagtatag cagagttgtt ttcagaagga
    23281 gaagatatct ttaggaacta tgagactcaa gcatgtagag aactttctat aagtgataaa
    23341 acagtgatat ctacaggtgg aggtgtaata aaaaaggata ttaatatgga aatactaaaa
    23401 gaaacaggta taataatttt tattgataga cctatacaaa agattttgga agatataaat
    23461 attaattcaa gaccattatt aaaaaacgga aaagatagat tgtataatct ttacaatgag
    23521 agaataaatc tttataaaaa attttcagat atagagattt taaatgataa atcattaaat
    23581 aatgctgtat ataatattac aaatgcagtc agtgaaaatt ttaaatttga ctttaaagaa
    23641 aaataatcta gggggaaata aaaataatga atattgttat tgtaggacta ggagttatag
    23701 gaggctcttt tgctaaagca ttaaaaaaag ctggatatga aaatgtattt ggagtagatg
    23761 tagatttaga aactttaaaa aaagcagaaa aagcaaaaat cataaaaaaa ggatgtacta
    23821 caggtaaaga gttatttaaa aaagctgatt taattatatt atctatatat cctagattag
    23881 ttgtagattt tctaaataat aataaaaact tttttaaaaa agggactata attactgata
    23941 ctacaggaat aaaagaaaca cttatcaatg atgtgttgca aattattcca gatgatatag
    24001 attttatatt tggacatcca atggcaggta gggaaaaaaa gggtattgat tttgcaagtg
    24061 aacaagtttt taatggcgca aattatataa ttactccaac tggcagaaac aacataaaaa
    24121 atttagagtt agttgaaaat ttaattttag agattggatt taaaagagta aaaaagttaa
    24181 cttcacaaaa acatgatgag attatagctt ttacttctca gttacctcat gttatggcag
    24241 ttgctcttat aaatagtgat gaagaaggta gagatacggg taaatttatt ggagatagtt
    24301 atagagattt aactagaatt gcaaatatga atgaagactt atggagtgaa ctttttttag
    24361 gaaatagaga taatttacta aaagttatag aaaactttga atcagaagta aatttagtaa
    24421 aagaagctat atttaacaat gataaaaata aattaataga gtattttaaa aaatcatcaa
    24481 taagaagaga agctttagaa aaataaatat taatatttaa g
//
LOCUS       DAMKMI010000047        23998 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMKMI010000047 DAMKMI010000000
VERSION     DAMKMI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.048"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     regulatory      126..227
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            300..1643
                     /locus_tag="PC429_001753"
     CDS             300..1643
                     /locus_tag="PC429_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF3847263.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     gene            1822..3186
                     /locus_tag="PC429_001754"
     CDS             1822..3186
                     /locus_tag="PC429_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3847264.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(3364..5229)
                     /locus_tag="PC429_001755"
     CDS             complement(3364..5229)
                     /locus_tag="PC429_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF3847265.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINILKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(5465..6682)
                     /locus_tag="PC429_001756"
     CDS             complement(5465..6682)
                     /locus_tag="PC429_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3847266.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            complement(6882..7745)
                     /gene="aroE"
                     /locus_tag="PC429_001757"
     CDS             complement(6882..7745)
                     /gene="aroE"
                     /locus_tag="PC429_001757"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF3847267.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            complement(7827..9749)
                     /locus_tag="PC429_001758"
     CDS             complement(7827..9749)
                     /locus_tag="PC429_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3847268.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            complement(9813..10661)
                     /locus_tag="PC429_001759"
     CDS             complement(9813..10661)
                     /locus_tag="PC429_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF3847269.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            11074..11955
                     /locus_tag="PC429_001760"
     CDS             11074..11955
                     /locus_tag="PC429_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3847270.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(12163..13026)
                     /locus_tag="PC429_001761"
     CDS             complement(12163..13026)
                     /locus_tag="PC429_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF3847271.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(13181..15115)
                     /locus_tag="PC429_001762"
     CDS             complement(13181..15115)
                     /locus_tag="PC429_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF3847272.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(15227..16240)
                     /gene="galE"
                     /locus_tag="PC429_001763"
     CDS             complement(15227..16240)
                     /gene="galE"
                     /locus_tag="PC429_001763"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF3847273.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(16264..17133)
                     /gene="galU"
                     /locus_tag="PC429_001764"
     CDS             complement(16264..17133)
                     /gene="galU"
                     /locus_tag="PC429_001764"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF3847274.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(17331..17651)
                     /locus_tag="PC429_001765"
     CDS             complement(17331..17651)
                     /locus_tag="PC429_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847275.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(17641..19632)
                     /locus_tag="PC429_001766"
     CDS             complement(17641..19632)
                     /locus_tag="PC429_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847276.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(19755..22298)
                     /locus_tag="PC429_001767"
     CDS             complement(19755..22298)
                     /locus_tag="PC429_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF3847277.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(22576..22910)
                     /gene="rnpB"
                     /locus_tag="PC429_001768"
     ncRNA           complement(22576..22910)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC429_001768"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            23042..23719
                     /gene="srtB"
                     /locus_tag="PC429_001769"
     CDS             23042..23719
                     /gene="srtB"
                     /locus_tag="PC429_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF3847278.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(23879..23953)
                     /locus_tag="PC429_001770"
     tRNA            complement(23879..23953)
                     /locus_tag="PC429_001770"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23919..23921),aa:Thr,seq:tgt)
BASE COUNT         7660 a         4149 c         2708 g         9481 t
ORIGIN      
        1 attcaattat tggaaaaagt aatttttttc aataaataac cttaattttt tataaaatcg
       61 acaaaaaaac gaataatttt attatattta ttgtaaataa tacaatatta cgatataata
      121 caacagaaaa acaaatagaa aatgttcata taatattagt aatacggtct aatagtttct
      181 acaaactcac cttaaatggg ttttctatga atagaatgtc aactctgaat atatgttcta
      241 ttctatctat atgaatatat atttttttgt ttatatctta aagttttttg gaggtaaaaa
      301 tggtaccaaa cactaacaat gtacaaccag gtggaaataa ttcatcatta agctttttag
      361 acaaaatgtt taaatttagc gaacgtggct ctagccttaa aacagaggta ataggtggag
      421 ttactacatt tttaacaatg gcatacatag ttttcgtaaa tccagcaata ttatctgaag
      481 ctggaatgga taaagccgcc cttataacag ttaccatact agctacagcc attggtacac
      541 ttatatttgc gattttagga aatgcaccat ttgctctagc tcctggtatg ggattaaatg
      601 cattcttcac atactctttg gttataggtg caggaatccc atggcaaaca ggtcttgggg
      661 tagtttttct atctggatgt gtcttcttta cactcgcagt aacaggtctt cgaaagacaa
      721 ttgcagacgc aattcctaga gaattggcag tagcatcttc agcaggtata ggtcttttcc
      781 ttgcttttgt tggtttaaaa aatatgggtg ttataatagc taatccagat actatagttt
      841 cacttgcaga ttttacacca acagttttaa tagctctttt tgctcttatc ttaatggcaa
      901 tactagaata taaaaaagtt aaaggtggaa tattgataag tatagtagtt gcaacaataa
      961 tatctgttat atttggattt gtggagttgc caacagcaat aatctcgaca cctcctagta
     1021 tagcaccagt tgcttttaaa ttagatattg taggtgcatt gaaattctca ttaattggac
     1081 ctatattctc tttcatgttt atagacatgt ttgattcatt agcatttctt attacttgtt
     1141 gtaaacaaat gggtcttgaa gatgagaatg gtgaaataaa agatttagga agaatgttat
     1201 atgcagatgt ttcttctact ttaataggct cattcttagg aactagtact gttacaacat
     1261 ttggagaatc tgcttctggt attgcagcag gtgcaagaac aggacttgct tcaatagtaa
     1321 cagcagcatt attcttagct acacttatat ttacaccatt acttggtata gttcctactt
     1381 acgctgcagc tcctgcactt gtaatggttg gtgtattcat gtttgaaagc ataaaacaga
     1441 ttgattttgc tgatagcaaa atagctgtac ctgcttttgt tacagtttta ttgatgcctt
     1501 taacttatag tataagtatt ggtttatgct ttggatttat ttcatatata ataatgcatg
     1561 tagtagctaa agaaactgat aaaatcagta ttacactttg gattataggt ttacttgcag
     1621 ttgtaaactt agttctaagt taatattttt aaatagttct aggtcaatat tttaaattat
     1681 atagaataaa atcttattta ttttaaattt ataaatagca aaatcacttt atatgtaaat
     1741 tgattttgct attaactttg tatagaaaat ttattatcat aatatataga tataaaaata
     1801 tatagaaaag gagtggtgtt tatgtcacaa ttacttataa aaaatgcata tctaattact
     1861 atgaatgcag atagagaggt atatgaaaat ggaagcatac ttattgaaaa taatattata
     1921 aaagaggttg gtaagtttga tattaatatt gtagataaag atgcagagat atatgatgct
     1981 aaaggaaaaa tactaatgcc agggctagtc aatactcatg ttcacttatc tcaacaatta
     2041 ggacgtggtc ttgcagatga tgttgtactt ttaacttggc ttagagaaag aatttggcca
     2101 tacgaaagta gcttcaatta tgaagattcc ttgatttcat ctactgcatg ttgtgttgaa
     2161 ttgataaaat ctggtgtcac tacattttta gaatctggtg gtcaatatgt agatgctatg
     2221 gttgaagctg ttgataaaac tggacttaga gcctgtttag ctaaatcagt tatggataca
     2281 ggagatggtc ttcctgaggc atggcaaaaa actactgatg aggaaatcaa cactcaatta
     2341 gacttattcc ataagtttaa taatacatct aatgagagaa ttaaaatatg gtttgcatta
     2401 agaactattt ttaatacaac tgaagaatta cttgttagaa ctaaagaact agctgataaa
     2461 tacaatacag gtatacatat gcatattgct gaaatagctg atgaaatttc atttgtaaag
     2521 caaaacaatg gagttggaac agttgaatac cttgataaac taggtgtatt aggaccaaat
     2581 cttttagctg ctcatacagt ttggcttact cataaggaaa ttgatttatt tagattacat
     2641 gatgtaaaag tttctcatga cccaggttca gctatgaaag tggttctagg gtttgcatca
     2701 attcctgaaa tgttagaaaa aggaatacct gtttcaattg gtactgatgg agctccttca
     2761 aataatagaa tggaccttat gagggacatg tatcttacat cactaataca taaaggaaga
     2821 actttaaacc caactgtcgt accagctgaa gaagtcttag aaatggctac tataaatgga
     2881 gcaaaatgtg ctttactaga aaaagaaatt ggtagtcttg aagtaggaaa aaaagctgat
     2941 atgattattc taaatccaga tacaattcat tgtttaccaa tgcataatcc aattggaaat
     3001 atagtttatt ctatgactag tgaaaatgta gactctacta tttgtgatgg taaatggttg
     3061 atgaaggaaa gaaaagtatt agttgtagat gaatctgaat tattaaataa agttaaaaaa
     3121 caagcttcta aaataaaaga taaagcagaa gttaaaattc catctaaatt taaaattata
     3181 aaataacata aatttcaatt gttttgtaaa tatgaatata taaatatatt tatatacatc
     3241 atatatacaa aacaattgtt tttttatttt atattcatta gcattgctca taaaatacac
     3301 cataattcca gaaaaaataa ccaatcagtt aggattacat catctaagca atatataaac
     3361 gtcttagttt ataagtttta ttctactctt atctaaaacc atctttaaag agttttcaac
     3421 tgtttcaatt acatctttat aactcaagta cacatttgcg tagatacaat cttcatataa
     3481 attatattca gttactctac aatacagcag ttttcctcca tctaataaat tgctcaaaat
     3541 atagttttct tttcttggga catagcctat cacatatcca gtttcaccaa ctattttaat
     3601 agctttttca tcatatagat tatctttctc tctagttaaa tagtatatct tacttttctc
     3661 caattctttt tcaagatatt ctctatttct aaaccttgaa ccagcaacag ctattgaagt
     3721 caaatagata tccttaccat gttcatctac ttttattttt tcaatattta caaattcttt
     3781 ttttaaatta ttactttcat ataatactct tcttaattca tccttcaata taacattttt
     3841 ttctttttca tacgcttctt taacaatttt taattcttca agagatagtt tctctctgta
     3901 tctttttaag atatttatgg attcttttct tacttcattt atatttccaa atattccttt
     3961 taaagatata ttctttcctt caggatatag tgatttactt cctttaagca caataaaaaa
     4021 taacatatca tcataatatt cttgagtcaa tttatctttt tctatatctt tcatttttcc
     4081 aattaaatca tctattttaa acgtctcttc aaaaaaattt aataatttta atctagaact
     4141 tgtcgttccc tttttagata tatgccaata tacctcaaaa tctctaatat ctctatttag
     4201 atatggcaac aagtcatcaa aagataagtt tacatcataa atttctgaca tttttataat
     4261 atcttctgtt tttccactag catctcttaa tgcttcaaaa tatatatttt tccatttttc
     4321 actgagcaag ataccattta tttctttttc aaattcaatc ttattgcact ctatattttc
     4381 atcttttaag atgtttatag ctattaggaa aattgaatat aaactttcaa agttagtccc
     4441 ataatttact acagtaggta aaaatcgatt cactaattct aatttattac ctatatattt
     4501 aaattctaca cttagcaagt aattgcatat aagacgtgct aaattattta tcttttcttt
     4561 atctaaatct cgtttttcaa gatattcatt caaatctaca attgaaataa tatagttcat
     4621 caataatcgt tcgtactttg tatccttata accatcttca attagatagg aatttatttt
     4681 agaatctaaa ttttccatat taacaatagc aaatactttt attgaacctg tagccttttt
     4741 agataaatta aataaatatg tattgtaaaa ttcaagtttt ttaatagtat tactcagata
     4801 aaatacgtat tctccagatt tagaaaatgt atctacaact tctcttaagt tttctacatt
     4861 tagatatttc tcacttaaaa ctaaccctag ttttacatac tcaacttttt tactcttata
     4921 tattaattct ttagttaatt tatataacca tttctcatca ataatcattt cttttaccaa
     4981 agtatttatc tttttcataa aaatttcgaa ataacaaatt aaagacttag aattactacc
     5041 ctccaataaa taaatttcat tagattcact tttatacata tttaaaattt catctactag
     5101 attatttgca agctctactt gttctcttaa cggtatactt tcatatatac ataggtaatt
     5161 catttcaaat gattctttgt atccatcata accattaaat tcctgcaatc gcttaaaaac
     5221 attctccacc aaattatcct cctttacatg atgtaattta tattgattat actacttatt
     5281 tcatcaagtt tacatttata cgcaaaaaaa tattactaga aattgaaatt ctagtaatat
     5341 ccttattatt aattatgtaa ataccctatc ttataactgt aaataatatc cttattaatt
     5401 ttaattactt ttaataaaaa tctatcctag atttagactt ctctatgcat atattatact
     5461 aatattagac atcataatca tacacttttt ctttttcata tctcatgttt acaaacaatg
     5521 caagtaaaat tccaacaaat gttattgcca cattaaacaa tacaacatat tttggtccat
     5581 taactccccc tgattttgta ataatacctg ctatatttaa aataacatag tttgctatac
     5641 tagatgcaat cataactata gatgttattt ttcctttatt agttggaaac atttcatttg
     5701 cagttgacgt tgttaactga agaacacctc ctgctgctga ataacctatt acaaatcccc
     5761 ctaacataca aatagttgga gtttgaacaa aatatacaac taatagcata agtaaagcta
     5821 ttataggata tataactaat attcttattg gtttaatata ttttttaata agtaaagaag
     5881 ttattaatac tgcacatatt actcccattg agtaaaagga ttgaattttt gtagggtcag
     5941 ccatcccata caatttaccc aattcctgat tacaatttaa ccataataca aatgtagatg
     6001 tacatgtaaa tccaatacaa acaagagcta tacttgttgg tgtaaatttc atcttctctg
     6061 atttaactgt tttgcctttg ttatcaataa cattattagc tggtggaaat ggtaacacag
     6121 ctatcaaaaa ggcatctact acaataagaa tcgctgttac tataaaaata actttaaatg
     6181 atattgaatt tgctgcaaca aatccaatta taaaaggaag caaaaattgt gctaaagcta
     6241 ttgtaaattt agtaaacata tttgctattg ttcctaaact agcaaatatc tccatacaag
     6301 aaggagttac acaggtatct aagaatgaat ttgctgcccc tccaattatt gcaaatacat
     6361 atgctatata aaaatttggt gaaaatacaa ttccaacaaa gaaaattgca tgtagaaagt
     6421 ttcctataag accacttact tttcttccat acttatctga aataggtcct gcaaatggat
     6481 aagatataag tctccctaat cctagtgcag caataacagc taaaacaata cttacatcca
     6541 atgttccatc tgataatgtt ttagcccccc acattgatga aaattctggt ttgtattgtc
     6601 ctaaaattga aattccaata ccaagtaaaa aatatgaaaa atacaacgct atggcagtag
     6661 gataatattt tttattgttc ataatttttc tcccattctt gttaatttat taacattttt
     6721 gtgtacaata aactcatccc cataatggat ttaaagtata gtcagttaaa acttcaaatt
     6781 taactggact atgaggcaaa acccactatg acaatttgta atttattatt aaaaatattg
     6841 aaatacgtat gttttttcat tgtttaagat attactctta tctatttaaa gcataattca
     6901 ttaacttctt ctacaggcat ttctaatcca gtatatagat taaaagcttc tgctccttgg
     6961 tataataaca ttcctaatcc accaatagcc ttacatccat tagcttcagc atcttcaatc
     7021 atttttgttt tcttaggatt ataaacgaca tctgttacta ctaaatcttt tcttaaaaca
     7081 ctagtatctt ttatatttgt ttcattgtca taaggcttca taccaataag agttgcattt
     7141 gtcaatatat cacttgattg tatctcctca taaagtttgt ttgtatcttc taaatcatat
     7201 agattaacaa cacattctgg tacatctttt tttatattct caactgtttg ctctgctctt
     7261 ttataaaaat catccttagg attaaagata gatatttctc ttgctccatc taaagcacat
     7321 tgtacttgta tagcagttgc tgcaccacca gcacccataa ttgtaatctt tttaccttta
     7381 acttctaccc cattttcttt taaattacga acatagccta ctccatctgt tatatgacca
     7441 actaactttc cattgtcatt tacaattgta ttacatgctc caattatacg agctgctgga
     7501 gaaagttcat ccatatattc tgtaacagca gatttacatg gcatagtaac attggctcct
     7561 tttatattaa aagtttttat tgctaaaagt gcctctttta ccttatctac tgtgatatca
     7621 aaggctaaat atctataatc taaatctaat ttcttaaaac tataattata catcactggt
     7681 gatttagagt gaccaaccgg tgtacctatt aatgcaaata atcctgttct acctgaaatt
     7741 tccataacta taatctcctc tcaatattat cattttcttt gcaaataacc tattcagtta
     7801 tttacaaaga aaaatttatt tattatttat atatttaatg ctacttcaaa agcttctttt
     7861 gttgcatcta atgccctacg agcttttact gcatcaccaa caatataagt ttcttttaca
     7921 acttctttta taacttcact taatgggtcg taattacgag aacccatagc aagtactact
     7981 gaatcaaaac cttttaaatc atgctctgtg ccatctgtaa gtaaatagtc gacaccatca
     8041 tcataaaatt tagaaacttt tgcaccagta atggtatgta tattataatt ttcaaaatca
     8101 ttcataagga atttacggtg ttctgaaatt acatctgcac caacttcttc tctaagctca
     8161 ataacagtaa cctcatgttc ttgttctcca aggaaggctg ccacttcaca tccaaccatt
     8221 ccacctccaa caacaagtac ttttttccca caagattttt taccatcaag aaggtctaca
     8281 gcatgaatta atcctgaatc attgattcct ggtataggaa gtacaagtgg agttgctcca
     8341 gtagctataa taactacatc aggtgatttc tcctttataa gttctactgt agcttctgta
     8401 ttagtacata tttttacacc atattgatta cattttgaaa tataacttct aactaagtta
     8461 gtaatatctc ctttccctgg aggatatgca gctaaacgca tttgcccacc aagaacttca
     8521 gatttttcaa ctaaagttac attatgtcct cttttagcac atacccatgc tgcaagcata
     8581 cctccaacac ctccaccaac aacaagaact tctttgctaa tttcagctgg cttaagctct
     8641 gcttcatgtc ctaatatagg atttgcaaga caggctatag gtttgccttg gaacatattt
     8701 ggtacacatc cttgtaagca tgcaatacat ggtatcattt cgtctagttt tccattttta
     8761 gctttattag gcatttctgg gtcagccaag ctttgtctac cgaatgctac taaatcacat
     8821 cttccttgtc tgaccattaa ctcagcaaac tgtggctctg tataacgtcc aactgttatt
     8881 actggtatac taacagcttt ttttatttca gtaactaatt ctgcactgaa tccagcatgt
     8941 aaagtcgtag gagcccacat atattcatca tgtatatgta tactacgtga aacatgaagt
     9001 ccatctattc cacaatcttc tagatatgct gctattactg aactatcgtg tacatcaatt
     9061 cctcctggaa catcatcagt gctgtttata cgacataata ttgctagaga atttcctact
     9121 tttttacgta tattttcaat gataagacga ggtaatctca ttctattttc aaaacatcca
     9181 ccaaactcat ctacacgctt atttgttctt tgtgatataa aactactaac cagatatcca
     9241 tgagcgcagt gaacttcaac agcatctgca ccagcttttt gtgcacgtaa tgcagcatct
     9301 ccataagatt ctataagttc atatatttct tctgttgtta ttgctagtgg tgtattacga
     9361 ccatttgatg caggtattgc agaagcagct tttaatggat gtcctgctac tttttcattt
     9421 ccttcagaac cagcatgttg taattgtata gataccttcg aacctgcatc atgacaagca
     9481 tctattacat ttttaaaact atctattgtg ctatcatcat ataaacaagg cttacgagga
     9541 cctccttttg cttttttgtc tacaacacta gattctatag ttattaatcc aaaaccacct
     9601 aaagcacgct caagataata agccttagaa gtttcactta aagtaccatc tgtgtttgca
     9661 aaattgtttc ccataggggg aactacaaaa cgatttggaa ctgtcattga accaattctt
     9721 ataggtgtaa acatagaact aatcttcata aggttttcct tcctttcaag ttttatatta
     9781 taatactctg tatattttta tattatataa gtttattttg gagatacttc agaccatgct
     9841 ttatccaaaa cttttttagt tgcattaaat acagtttcag cagcagtgga taacccttta
     9901 gatattagag aatcagatat tacttctgcc cctatgactc ttggtgatat attgtgttct
     9961 tttattattt tcacaaattc ctctgtgttt ccatatcctt cacctggagc caaacggtca
    10021 tgtagtgatt catccctaag tttttcataa ggagtttcta aaacatcaca cacttgaatt
    10081 gatactatct tatctgctgg aacaggtgca aggtcctctg ctgtctgctt tgctctagcc
    10141 cagtgccaag tatctaatat aagcattgca ttttcacgat tacatccttt cacaacacgc
    10201 catgcagtag ccaaatctgg tacaccacta tatggcataa actcaagacc tataataagt
    10261 tcacctgctc tgtcacataa ttctcccaat gcagttataa tttgttcttc tggtatcatc
    10321 tctaataatc cacaatttat atgtttaaca ttaaataagt gagccatatg ataaacattt
    10381 tgctcttttt ctctttgagc ttccgttcta tcttcttctc ttccccattg agttatatat
    10441 tctacttctg taactttcat atcatattca ttaagaattg caatcatttc ttcatctgta
    10501 acacctgcat tttttgcatc aatataattt tcagcacgca atccaatacc atcaaaccca
    10561 gcttcttttg caatacgaac tctatcacgg aatgaaactt ctgttcccaa tgtataagaa
    10621 ctaattgtaa ttggaatatt aagatttttt tgattattca tagcttaaat ttgccccctt
    10681 tattttcttt ataattaata gtatttttga ttttatattt ttatttatcc tactagtagt
    10741 attattttac cttaaaaata aaagtatttt ttgatttcta tttatctaga ttttataaat
    10801 ttattgtatc acgagacttt actaataaca aattgataat ttcctattaa ttcatagatt
    10861 ttgtttatag attttattta taagataatt cttgtatttt tctattaggt cgtataaata
    10921 tatttatctt ataattcaga caaaaaacga taaatttttt taaaagcttt tattcttact
    10981 ctataatcat atataataaa aatgtaaaaa tatcttaaag gttaacttct acaaatatat
    11041 aaataaaaat acctatgaaa ggatgaacaa aaattgaatt tatatcactt acgctatttt
    11101 gttacattgg cacatttaga acactataca aaagcggcag aaaatttatc tataacacaa
    11161 ccaagcttaa gtcatgctat ttctttacta gaaaatgaac ttggggtggc tctatttgaa
    11221 aaagaaggta gaaatatagt ccttactaag tatggaaaaa tatttttaaa agatgtggaa
    11281 aaatcattag aaattttaga ttcaagtgta aaatcattaa aaataactgg tactggtgaa
    11341 ggacaaatag acttagcttt tcttagaaca cttggtactg attttatccc agacatagtg
    11401 cacaaatttt taaaatctaa tcctgctaaa tcaatagatt ttaaatttca tactggtgta
    11461 actactgaca ttattcaagg tcttaaagaa agaaaatatg atattgcatt ttgttcgaaa
    11521 cttgaaaaag aaaagggaat tgaatttata cctgttgcca aacaagattt agtactaatt
    11581 gtaccatata gtcatccact agctgcaaag gatactatag atttaaaaga aaccatacct
    11641 tatcctcaaa tagtatttaa tcagagaagt ggtcttagat atataattga tgatatgttt
    11701 aaaaaaataa atcaacagcc taatatagtc tatgaagtag aggaagacca agtaattgct
    11761 ggattagttg caaaaaattt cggaattgct gtagtcccaa atatgaatat gcttagcttt
    11821 acaaaagtaa aagtaattca gattattcac ccaagttggg aaagaaactt ttacttagca
    11881 tttataaaag atagatattt gcctcctgca attaaaaact ttaaaaactt tgtcattaaa
    11941 aacgctcaat tataatttat taataaaggg gttattttta aatatcctct tttattcttt
    12001 ttataaaata aaaaaatcta tacttttaaa agtatagatt cctaactgac agtcggcatc
    12061 cgagtctcag taattatcgt tatacaacag caactggatt cagttcctga ataactaggt
    12121 tacttacatt atatcagatt ttttaaaatt tgtcaattgt atctaacgca ttttttcaag
    12181 ccatggtcca acaggctcta caataggaac ttctttaaat ccccaatttc ttatatcatt
    12241 cacactcgtt ggagtatata ttttgtttct gacagatttg aatattttgt ttttttctcc
    12301 atattgcata cactttggat tagcttctat taaccctctt cttgctctgc taagaggatt
    12361 acataaaata tctgctattt ctaaaccttc tctatagtca gcatcttctt ttttggcaat
    12421 tataaaaccc ttaaccttat ctcttaattc ttcatctata tctaagtata ttgttccatt
    12481 attaaataca tcaaaaaacg ccttttgtac ttttaagttt gagacatcat cccttgactc
    12541 tataactatt cgtgcagatt ctacagtttc attatttaca aatgaataaa aattctgaag
    12601 aacatgtata aaagccacaa catacggttc ctttgaaggt gcataatatt tctttaatct
    12661 agctttatct actgttactg atattatagt acactctaat ctagatagaa ataaaggcaa
    12721 ctcatcatag aataacttaa tctgtgaatc atcaaacttc tcaaatgctc ttgattttct
    12781 tgatatatct gtcaaatgta ttcctatgtt actggttcca aaacaatact ctttaaatgc
    12841 atccatttct ttcctcaaag aacatacata atcgccttga cttactgcat atttatattc
    12901 aaaaattact cctgtcaatg taaatgggtc ttcatgattt atatcagctc ttccactttc
    12961 atcaataaac attatatatt ttttctttat aatcttactc accctcttaa catatgatga
    13021 tagcatctat attcatttta tatgagtttt aaaaatattc caagattttt atataactta
    13081 aacttaattt tatactaaaa aattgagatg taaaacaatc aagcttttat tgattgaatt
    13141 tcacatctca attataatta tttttcaata tatattttta ctattttgca agaatatcca
    13201 ttaatttatc cattactata taagatactc cgcctcctat ttgataagga gtttcaactt
    13261 ttttatttgc aaatactctt tgttgctctg atgataatcc tgaattatcc attaaaacta
    13321 ttggtgaatg taattttaca gctaatggtc cagctgataa agcatctact aaagcatctg
    13381 atttactaac taatacagcc tgtaaatcag tatctccata gaatttttct atcactttgg
    13441 cattagtatc tactctacta tctccatata ttctatttcc tgaaatatct tcagtagtta
    13501 tatcattcat ttttgctatt actgaatcgt taattgcact tggtccacct ataaagtatg
    13561 cattttctaa ttttctttct gttagccatt tataagattc ttctgtaatt ccatctttat
    13621 tgactaatat tataggttgt ttttcttccc cagctttaga agctacagat aaagcatctg
    13681 cctccccatt tccagaaacc atatatgcag tttttatttc ttctcgattt ccaagttctt
    13741 cagctattct tgtagcagtt tgatatctat cttgacctgc tattctcttt gtttctgtgt
    13801 tgaaagtagt ttttatttct tgctctaatt tagaacttat tgcattttct ccacctatca
    13861 agattactgt tttaggtgct aataacttca tttcttcaac agtttcagtt ggtaaattag
    13921 ctgtattagt aagtagtatt gttgagtcat agctacttgc taaaggagtt gctgttatac
    13981 catcagctat tgcattacca tttactaata ttacagtatc tgaactatca gcattccagt
    14041 ttccaactga acttatcttt ttagctgtat caaatctatt tgcacctata attgtatccc
    14101 atgtaggatt tatagttcca tcttcattaa aagtataatc tttcccatct attttttgag
    14161 aaccagttag catctttcca tctttgtcta aatagtaaga cttaccatct atttgttgcc
    14221 atcctgtctg cattattcct gttttgtcaa agtaatactt attaccatct atttcttgcc
    14281 atcctgttac tgctaaacca tcagatccta gatagtattt ctttccattc ttttcttgcc
    14341 atcctgtttg cattactcca tctgagttaa agtaataagt ttttccgtct attactttcc
    14401 atccagatac tcttgctcca tcagttccta aataatattt tttgttatta attgttttcc
    14461 atcctgtttg cattactcct gaagcatcaa agtaatacat ttttccattt acttttagcc
    14521 aactattctt ttggtatgtt ccatcagatt ttacataata cttcttacca ttttctgttt
    14581 tccatcctgt ttgatttaga tttatcttat attttgccgc tatctttaca tagctgtcgt
    14641 tactataatc tataattaca tctgaaccta caggaacttg gtcaaataac catctaacat
    14701 ctttattgtg cattcttata catccaccag aaatatgctt tcctatagaa ctctcattat
    14761 tatttccatg tattccatat gtatctccat aagttccttt taatgctaat cccatccatc
    14821 tatctcctaa tggatttctt ggacttcctc caggtatatt acctttataa tatggtctat
    14881 tttttatctt gttaactact tttgttttcc ctgttggtgt ttctgacccc tttttacctg
    14941 ttgcaactct aaattctttt acaagtttat tatttacaaa atatcctaga gtattttttc
    15001 ttgaatttac tataatcaca tgtttactgg ctgcgcttga ttctggtacg ccacatgcaa
    15061 atactaaaac taaagtcagt gctagtacta aatacttttt tagacttttt ttcactataa
    15121 cttccccctc ttttagaaac attactacat gtgtctaatt ataacataaa attaccaata
    15181 ccttatattt acaaatttgt tacatttata catataagta tgtattctac tcttcatacc
    15241 cattaggatt catactttgc catctccatg aatcttcaca catctcttca agttcatact
    15301 tagctttcca tccaagctct ttttctgcct ttgaagaatc tgcataacac atggctacat
    15361 ctccagccct tcttcctaca atcttatatg gtatttcttt tccacttgct ttagaaaatg
    15421 cttttactaa atcaagaaca ctatacccct ttccagttcc taaattataa acaacaagtc
    15481 ctggattttc ttctaatttt tgtaatgctt ttacatgacc agcagctaaa tctaatacat
    15541 gtatatagtc tctaactcca gttccatcat gtgttggata gtcattacca taaacactca
    15601 attccttcaa tttacctact gctatttttg ttatataagg cattagattg ctcggaactc
    15661 catttggttc ttcacctatt ctaccacttt tatgagcacc gactggatta aagtatctta
    15721 aaagagctat atccaatgac ttatctgctt ttgatatatc tactaacatc tgctctatca
    15781 taagtttagt tcttccatat ggattggtta cagaaagtgg aaaatcctct aatataggac
    15841 aagtatgtgg gtctccatat acagtagctg aagaactaaa tacaaacttt ttaacaccat
    15901 attctctcat taattcaaat aaatttaagg tacttattag attattgctg taatattcaa
    15961 ttggtttttc tactgattca ccaactgctt ttaaagctgc aaagtgtata attgattcaa
    16021 tattattttc tttaaataca acatgcattt catctttatt tctgatatca atattataaa
    16081 attttactgg ttttttagat aattccttga ttctatcaac cactattgag ttgctattac
    16141 ttaagttatc aactataaca acttcataac ctgattctaa aagttctatt gctgtatggc
    16201 ttcctatata tcctgctcct ccagctacta aaactgccat tgtaattacc tcttttctaa
    16261 tatttatttt tcacttactt tttttctcat atagtccatt aaatcatctt ttaaattttc
    16321 tctctttaga gcaaagtcta ttgttgcctc taaaaaacca attttatctc ccacatcata
    16381 tcttctacct tcaaagttat aagcataaat agcctcttgt tttccaagag tttgaagtgc
    16441 atctgtcagt tgtatctctc cacctttacc aggtgtctgt ttctctaata tgctaaatat
    16501 tgcaggagtt attatatatc ttccaagtat agctatatca gatggagctt cttcaattgc
    16561 aggtttttca accatatctt taactttata tactctatct tcgatatgtt ttacatccaa
    16621 tataccatat ttattagtat cttctttagc tatcttttga acacctaata cagttgtttt
    16681 atactcatta taagtatcta ttaattgttt taagcacgga acatctgaat ctactatatc
    16741 atcacctaat aatacggcaa aaggttcatc acctataaaa ctttttgcac agtaaattgc
    16801 atgtccaaga ccttttggct ctttttgtct tatataatga atatttacca tatttgatat
    16861 gtctcttacc atttctaaca tctctgtctt acctttttgc tctagttcta actctaattc
    16921 aactgattta tcaaaatgat cttctataga ttttttattt ctacctgtta ttataagaat
    16981 ttcttcaatt cccgattcta tagcttcttc tattatatac tgtaaagttg gtttatctac
    17041 tataggaagc atttctttgg gctgtgattt tgttgctggt aaaaatctag ttcctagccc
    17101 tgctgctggt attattgctt ttttaactgt catctgcatt ttttcaccct tcttcgtaaa
    17161 ttttctattg taccaaatta atataccata tttatacttt ttcttcaaac tctatattat
    17221 cccaatattt aatgctataa gataatataa ggtagatgaa atttcatcta cctttagttt
    17281 tataagttaa tattgtattg tttatatttt atccaaaact tatacaggta ctaaatttca
    17341 tctaagatgc cttttatatc actatctata tcttcatcta gataagaatc taaatcttct
    17401 aagtcaactt ctagttcttg atatgtcata tcaacactga catcctcatc tatatccatt
    17461 tctgatatta attctcctaa aatactattt ccatcagaat ctaaaccact atctatatct
    17521 tcatctatat agttttcatt taaaatttca tctaaatcaa tctcgtaata aacttcatta
    17581 tttttttcat cccctgagtc caatcctaag aactcatagt taaaagtcca aacattgtat
    17641 ttacttgaca tactttattc ctcctgtgaa aaacatattt tcatctagtt tattggttcc
    17701 ataatcaaca gagttatata ttttttcaat tatgtctttt cttctttctt ttgtactatt
    17761 atttaatgtc aattgtttaa atacatatgt atttgttatt ctctcgaata atatttcttt
    17821 tatactatca tcatcaatat ttctatagtt tttccacaat aactcaaata acttaaacat
    17881 ttccctacta gtttcatcta cataataagg atacatattt tctactaaat ggtttaatct
    17941 tgatatggct tgttttaaaa atacatcaag acttgcactt tcactttctt ttaacattct
    18001 ataattgtat ttcttcttat cttctcctga aattacaaag atgatattgt ctaaactttt
    18061 ttcaacttca tttaaataac tgcttatatt atatgataaa tcagacaata gatttttaat
    18121 ctgcttaaac acttgtacat caagtttagt attatacttt tcaaacaaat ctatttcttg
    18181 attttctaat attcttggca tatatgctga caataaatct aaattgtttc tttgttcttg
    18241 tatttgctta taaagtgaat ttataacttc tattcttatt tcatcattta actctttttc
    18301 taaaatctta tcaaattcag atttcatata gtaaatattt ccaaagaaat taaatattat
    18361 catatcattc atagctttag accaattctt atatatcact tcaatagaat tttctttaag
    18421 tagaggttgc tttgcatata taaatacatc ttgcataagt ttttgaacca tacttttact
    18481 taagtttaaa agtacttttt gattattaaa ttttatttcc gtttctacaa aattagaatt
    18541 ttcgttttca agttctttat taattacaat gtcttttata tcactatttt ttatagatat
    18601 gatttctttt tgaaaagact ttagaatagt caagctatcg aaatcttctt gaataaaaaa
    18661 tggagcatgt tttaatgtat atcccactat ttcattttct atatctttta ggtcatctat
    18721 ttgcataatt tctctatttt tatcatttat atgtaggtta aatacatttt tattatcata
    18781 taatattata tcttctccta aatttaactt agaacaatta tctttcgctg actttacaat
    18841 agtcttctta gatttcttat ttataaaaat taattggtct tgatatttta ctaaaagcat
    18901 gtttgtgttt ttataatcaa atccatctat atttcctgat atcttgcaga atactatttc
    18961 acaatcttta ggaagatttc ctattttttg gttatcttta actctctcat ttaaatcaga
    19021 tgggactaca ctatctatat tgttattaaa tatagataca gaccttacta taatcccctc
    19081 tctttccata tgaaagtatc ccttcaatga cactctttta tcttctaaaa tgtatctttc
    19141 tatatcatca aaatttattt caaatctatt tttcccactg ataaatataa acttctcatt
    19201 atctttatta attccaccca tataaaactt attgtcatct aaacttaata tagtcatatt
    19261 atcctttaga tagaatataa attctctttg acttttgcag attaaatcat attcatccaa
    19321 agttattttc atagtcaaat ttgctgtaac atttttactt gaattttcac tttttatgta
    19381 aaaaggcaag ttatttccat agacaattat ttgagaatct tctaaaactg cctctaattt
    19441 ttctttattg tctgaaaaac tatatatata ttttacacct gtatcctcat taacttcatc
    19501 tgatttgctg attattatat tccctagatt tgttatatga agctttttat attcttttga
    19561 gtatctactt atgccactaa gctcaaaccc atctgaaagc tcatatatga ttttttctcc
    19621 taatttcaac ataccttcac cacctaaaca gaattataag tattttataa aggtgaataa
    19681 aatgtctcaa aacaaataat atttttttta atacttttta ttctgagaca tagtttctcc
    19741 ttttttataa tgctctaagc accttcaact atttcttcat aaagtttatc taaatgttta
    19801 acatattcac gatatccaga tttgttcttt aactcacttc ttaaatcttc atatttttgt
    19861 ttaagttctt cagcactcaa agaaggagct gattctttta attcttctaa cttataatca
    19921 tagtacttat catacatagc tatatatgta tcaatagact tcttagctag ttcattacat
    19981 ttttcttgtg cttctccata ttgagaataa tcacctattt ttattttata gtatccattt
    20041 tcttgaatta ttgttgaaga aataccttta gcttcatatt cttttgctgt ttcttttgct
    20101 gttttttcaa ctttaagtga acctgcataa acagtgtaat atacaccttt accatcttta
    20161 tatgcttcca tttcttttgc tttcttttca gcttgtgctt tttcttgagc ctctttttca
    20221 gcttttgcct tttcttctgc ttcttttttc tctttttcag cttgtgcttt tttctcttct
    20281 tcagttaatt tattgttagc ttgttgttga gttgatgact ttggaatatt ctctttatca
    20341 tttttcttat tgttgcctac tgttataaaa tatcctacac caacaataac cactataact
    20401 actgctataa tagtttcttt taatctactc ttcttagaaa caacctcttc atcatctaaa
    20461 gaatcttctt tatctgatac ataatttttc atttctttct tgttttctct atatttgtcc
    20521 ttagaagaat ttttagattt tttattacta ttttctttct ttttaatagg tattactact
    20581 gatttttcat tttctaattt ttcttctttt atttcttttg cttttttatc ttctaagttt
    20641 ttactttctt tttcaagctt cgattcatta tttgaagata ctttttcatc taagttatta
    20701 tttattggtt tttctatttt tacactacgt tcttcttcta tatgttgact tggttcttct
    20761 tgttttattt ctttcaattc attttgctct atttcttctg atttcacttg atgattttca
    20821 actggtttat ctaaaattat atctggagaa atatcattta aattctcttc ttcttgttca
    20881 gcttcttcat tgtctttttt aataaatcca aataatttag ccttcttttt actcttttta
    20941 ttttctggtt caattatatc ttctttattg ctgtcgtact ctttatctgt agtatcttta
    21001 tcttctacaa cgtcacctat atcttgatta ttactatcta cttcttcttt agtcccttct
    21061 tcagcatcat cttctattga aatactactt ctaaagcaaa tatctttatc tgcatttttt
    21121 atcatgtaaa tgtattcttc aatatcgata tctttatttc ttctagttct ttttgatgca
    21181 atttcatgat tttctaattc actatagtta tcttttatta gattctgtgc tttattccat
    21241 gtatcttcac taatttctgt tttattttta attacatagt ccacaaattc cttaaaattt
    21301 tcaggaccaa attctccaat tatagtcagt ctcattgcac cttctataag gtctctattt
    21361 cttctcttta aaaattctaa tacaactgtt gcacaagcaa aatctttaat aatttctttg
    21421 ccaaaaccat atgcttcaac agaatgaagt tcttctaatc cacataactt cttatattca
    21481 ttgtgtattg tttgagatat actttcatta tttaagctat ttaacttatc tataaacttc
    21541 gattctatat catttgattt tgattcatgt tttacttttg aatttttaat ttcatttagt
    21601 agcatgtcat tttctgaatt atctttacct aattgcaaat cctcaatttc agttgctttt
    21661 atttctactt tgttttgttc gttattctct tccacattat taggtaccac atctttatta
    21721 ctagctaata ttttgtttac acagaatata atttctcttt ttaattctct tttcgcatct
    21781 gcttgatttt taagaaataa atctccacat tctttctttt ctaaacttat ttttctaaga
    21841 gatatattaa gaaggtcttt atataggttt aaaaccttat aatccttttc taaacgtctt
    21901 cttctctctg atagttccat tttatcataa gctaaaaaac tatttaaatc attggctttt
    21961 agagagtcta atccctcttt catagcctta tttacatact ttctacattt tgctgtagtt
    22021 aactctaaat ctttttctaa ttcatctatt tcaggtatgt acttatctac tatccatttg
    22081 ccaataagtg gttctgtatt tcttgctaat gtcttataaa acaatatatt aaattttaaa
    22141 agctctgcat attttgcttc attaaattct actttttcaa atttcttttc caaaacacca
    22201 tctagctttt catttatttt agactcttgt tttaaaaaac ttgctgtgac atttaaaaca
    22261 gtgtttctta ttatattatt ttcactcaaa accttcactc cttaaatcaa ttattttatt
    22321 gtaatattta cattgtgcaa agttgctaat ttgattatga tatctaaata attatttact
    22381 aaatttgcta catcttatat catatcatat tatttacatc attttacttt ttctttttat
    22441 atctatctta tattatacca tttttaatat ctgtactaac ttatattaca atattcctca
    22501 aaattttaaa agtagattag acaaatttac aaataaaaaa acatcagttt aaactgatgt
    22561 ttttgactat cgaaataatt aagcaagtct ataagcggag ttctgtatta gataatcatc
    22621 tttctaggcc tattgtcacc aataggctca agcgacctac ctaccggaga gtacgagcag
    22681 cactctttat tcctatcttg gtcttgctcc aggtggggtt tacatagcca actagtcgcc
    22741 tagctgctgg tgagctctta cctcgccttt ccatccttac cacaatagtg gcggtttatt
    22801 tctgttgcac tatccttagg atcgctccca ctaggcgtta cctagcaccc tgctctatgg
    22861 agctccgact ttcctcatgt aagattacat gcgattatct ggcttactta atatttattt
    22921 tacttagtta tgttaacaca aattctttta catgtcaaag taatattata ctaaaaattt
    22981 aaatttattc aaaaatagtg taatatagat atataaacta catttaactc gaggtgatct
    23041 attgaaaaaa ttatatagaa tagtaattaa cattatacta gtattagtaa tactttacag
    23101 tggatttaat atatactcaa aacttaccaa atacaatcat gatactaaaa tatcctcaga
    23161 acttcaaaaa aaagaatata aaaaagagga tttatcaaag attaacagtg attttaaatt
    23221 ttggctttct gttgagaata caaatataaa ttatccagtt gtacaatcta aagataattc
    23281 ttactatctt gacaaagatt tttataaaaa agattctatt tctggtacac tgtttatgga
    23341 ttatagaaat aaatctattg atgacaaaaa tatcataata tatggacata atatgaaaaa
    23401 taaaactatg ttcaacaacc taaataagtt taaagatgct gacttcttta aaaaaaataa
    23461 taaaataaaa ataaccttga atggaaaaga atttttatat gatgtttttt ctgcttatat
    23521 tgtagaatct gattatgatt atctaaaaac aaattttaac aatgaatctg attatcaaaa
    23581 ttacataaat gatattactt ctaaatcatt gtataaatct cctattaagg taaatagcaa
    23641 tgataaaata gttacacttt ctacatgtac ctatgaattt gatgatgcca gaatggtgat
    23701 tcatggtaga ttgatttaaa actcacagac acagtttaaa acaataattt taaactgtgt
    23761 catcttttat acctataatt tgtaaattag accactaaaa gtatttaata caaaataaca
    23821 aatacatcaa acaataaaaa acaataaaaa aagactatgc taaaaacata gtctttactg
    23881 gagctggtga taggaatcga acctacaacc tgctgattac aagtcagctg ctctaccgtt
    23941 gagccacacc agcacaccta ttttattgta aattagcaaa actaattttt aaaagtta
//
LOCUS       DAMKMI010000048        23390 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMKMI010000048 DAMKMI010000000
VERSION     DAMKMI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23390
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.049"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            187..1182
                     /gene="ilvC"
                     /locus_tag="PC429_001771"
     CDS             187..1182
                     /gene="ilvC"
                     /locus_tag="PC429_001771"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF3847279.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VMIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDHT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            1235..2923
                     /gene="ilvB"
                     /locus_tag="PC429_001772"
     CDS             1235..2923
                     /gene="ilvB"
                     /locus_tag="PC429_001772"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF3847280.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSIEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(3284..4414)
                     /locus_tag="PC429_001773"
     CDS             complement(3284..4414)
                     /locus_tag="PC429_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF3847281.1"
                     /translation="MKGGVRKRSNKWYYYFDLGIVEGKRKKVERVGGNTKKEAEKALR
                     EALNEYENSGIVFEESNISLSDYLDFWYKEYVLLNCKYNTQESYRINIEKHIKPKLGA
                     YKVKALTPAILQNFINKKYKDDYSQNTLQVLKAILHRSLKSAVHPYKHIRENPMQYVS
                     IPKTKSKTETNKVKTITLEEFNQILNIFPQDSFQRIVLLIGFHTGMRRGEIIALKWDN
                     IDLDNKTITVKHTLIKKPNGMFELGQPKTESSCRTIFTGDTLIKALKEHKLYQKKMKL
                     KYGEFYFDSDWVCTKENGQQVNTHTLDTIVRQIRVALNNDFHFHSLRHAHATLLLENG
                     ANIKDIQNRLGHSQLSTTMDTYSHVTDKMKNETVDIFEKITN"
     gene            complement(4654..5160)
                     /locus_tag="PC429_001774"
     CDS             complement(4654..5160)
                     /locus_tag="PC429_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDF3847282.1"
                     /translation="MNKLDALLDLANNEEIEIYYTDKIADDIKGLYINRQGLKIISLL
                     NSLKQNNAKLIEILAEELGHHFTSVGNYVSSKNSYKNKILIDKTENKALKWACEFLIT
                     EEEIIHVINSHATSVYEIAEELQVSINFLLKRLEFLSKKKSMLDLGNNRFLVLTNLPN
                     FYIYEDIF"
     gene            complement(5423..7012)
                     /locus_tag="PC429_001775"
     CDS             complement(5423..7012)
                     /locus_tag="PC429_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF3847283.1"
                     /translation="MKSIISDVINRFNATPFLFVGSGLTRRYYNLPNWEDLLKVFAEK
                     ISNDDFIYTSYKNKAKSENPKMGINPRIAELIENDFNKKWFSDSSIRSLDSKYLDIVK
                     SGVSPFKAEIAMYIKNNSKIVDKYQDEVEKLSNISKKSLSGFITTNYDCFLETIVDNY
                     TTYIGQENLVFSSIQGIAEIYKIHGCVSSPNSIVINEADYIDFDSKSAYLAAKLMTIF
                     VEFPIIFIGYSVTDVNIKKILNAIVNCLSNENAKKLEERFIFIEYEKNFNDIEISSHT
                     IVFDNKMITMTKIKLEDFNLLYEALSEKKSKLPVRILRMFKQEFYDFTITNKPTAKIR
                     VGSVDDTRIKDEDLVLAVGKASDFGLKGLKGLSFDEWYRDIVMNDLEFSSDELLEYAY
                     PSLIKQYNKLPLNKHLFNSTLEFPDYRTIALESDFENIISNSIKKNRNNTYIKNRSVY
                     GIWNDEHQSFEKATRLIAFLEEKEIDTEQLENLLKKIFEENPNILESAKTSEKTNLRR
                     LIRIYDYMKYSNMQKSLGYSE"
     gene            complement(7036..7599)
                     /locus_tag="PC429_001776"
     CDS             complement(7036..7599)
                     /locus_tag="PC429_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF3847284.1"
                     /translation="MSILSDRLKFLRKEKGVMQKEIANYLNITTSAYGFYEQGKRTPT
                     PEMLSSLAEYFGTTVDYLIGRYDNKASNISSKTSCNNTLFQKRLKELRAEKNMTQEDV
                     ANKLNLTKSAYGYYEQGKTVPDAYMLSSLAEIFNVTTDYLLGRSIVKNDIDTVAAHRV
                     NPHKDLPEEAQEQLNDYIEFLINKYKK"
     gene            7752..7973
                     /locus_tag="PC429_001777"
     CDS             7752..7973
                     /locus_tag="PC429_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847285.1"
                     /translation="MNNLKELRTERNLKQKDVALKVGITTSYYGMIESGTRVPSLAIA
                     IKLSKCFGVSIEKIFLNTNTTRCCLKERS"
     gene            7976..8791
                     /locus_tag="PC429_001778"
     CDS             7976..8791
                     /locus_tag="PC429_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDF3847286.1"
                     /translation="MKDLKIVKVNNKLTTDSRDIALMVEKEHKILLRDIRNYINQMEE
                     ANKNMSTDLYPSDYFIENTYLDDYKREKPCYAITKIGCDFIANKMTGIKGTAFTGIYT
                     KKFEEMEQVLKNEQPKLPTTYKEALQHLIEQVEVNEKLQLEGKMKDQVIKELKPKADY
                     TDMILKNKGLVTITQIAKDYGMSGKEMNKILHERGIQYKQSGQWLLYKQHQGKGYTHS
                     ETIDITRSDGMTDVKMTTKWTQKGRLFLYDLLKVNNILPDIEKEYSYQTSMLC"
     gene            complement(8970..9149)
                     /locus_tag="PC429_001779"
     CDS             complement(8970..9149)
                     /locus_tag="PC429_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF3847287.1"
                     /translation="MSTSLPKYTLRINRVLLEKIKYIAESEGRSANKEIEQIIKKHIE
                     DYEQRKGEIKINIEE"
     gene            9285..9464
                     /locus_tag="PC429_001780"
     CDS             9285..9464
                     /locus_tag="PC429_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847288.1"
                     /translation="MNSNKKRITVRMPEKLNEEITKKSKYLGLTKNSFILDILWKEFE
                     LLEYRSYKKEVDKHE"
     gene            9457..10248
                     /locus_tag="PC429_001781"
     CDS             9457..10248
                     /locus_tag="PC429_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDF3847289.1"
                     /translation="MNNLQVIERNNERVLTTQQLADVYETDSRNISNNFNNNKDKFIE
                     GKHYFLLQGDDLKNFKGIHTEYENLKFASKMYLWTERGANRHCKILDTDKAWEQFDNL
                     EETYFKVKQHKPTCIEDVLIESLKEMKDLRLQVNQANSIALEAKTEVETIKDVVSLDS
                     NSWRTNTHQLIARIAKKQGGFEHINMLRTESYELLNKRFGVDLHRRLINKRRKMAEEG
                     VSESKREKVNNLDVIQDDKKLIEGYVAIVKDMALKYGISSDLSKN"
     gene            complement(10331..10525)
                     /locus_tag="PC429_001782"
     CDS             complement(10331..10525)
                     /locus_tag="PC429_001782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847290.1"
                     /translation="MKFVYVKIFNNWIDITDIGSINGCNAIEYINENLDKMYSSNYVS
                     INYKDKNYCIHPSCIQIVTK"
     gene            complement(10681..10899)
                     /locus_tag="PC429_001783"
     CDS             complement(10681..10899)
                     /locus_tag="PC429_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847291.1"
                     /translation="MTKFKDNIKLVRKQMNMTQKQFASLFGISERAYQYYEAGSREPN
                     LETLILISNKLNVSTDFLLGLSPNQNRN"
     gene            11012..11206
                     /locus_tag="PC429_001784"
     CDS             11012..11206
                     /locus_tag="PC429_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847292.1"
                     /translation="MKINNNLKLQREKIGLTQLEVAQKAKITERSYQYYEAGERLPNI
                     RTALKIAIILNTNCEKLFNE"
     gene            11248..12042
                     /locus_tag="PC429_001785"
     CDS             11248..12042
                     /locus_tag="PC429_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HDF3847293.1"
                     /translation="MNNLQLNNKNTITTLEIADMLEIRHWEVLRKLEGTEKTKGIIDI
                     LNDNNFVVVDYFIKSTYLDSKNESRPCYNVTKLGCDFLANKFTGEKGIIFTARYVRRF
                     NEMERVLKEQQPKLPTTYKEALQQLLVEVEEKEQLQLENQEKDKVIQLQQPKVLFADA
                     VSASNNSILVGELAKLIKQNGVDIGQNRLFTWMRENGYLIKRKGEDYNIPTQKSMNLR
                     IMEVKKRVINNPDGSTKVTRTVKITGKGQVYFVNKFKSSKQLSMLS"
     gene            12099..12308
                     /locus_tag="PC429_001786"
     CDS             12099..12308
                     /locus_tag="PC429_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847294.1"
                     /translation="MENKKIEEIEKRITMLENQLQAQLFSQITAQSNSDFIDYMYQCI
                     VLYLHEMNKSEKRKISEITKERIRK"
     gene            complement(12318..12692)
                     /locus_tag="PC429_001787"
     CDS             complement(12318..12692)
                     /locus_tag="PC429_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847295.1"
                     /translation="MQFSNNISKELQESIRKTVSDTSNTEYFYYAEFQYKIILKSIEE
                     FEKELDDEHEIALKLTNFGKDVLMIVEEVGYHNPCLIHYYGIVNGVYSEILQHTSQIN
                     FMITSVKKTDPSKPARRIGFIL"
     gene            12768..12965
                     /locus_tag="PC429_001788"
     CDS             12768..12965
                     /locus_tag="PC429_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847296.1"
                     /translation="MLIGDNIKQILRKRDIKPYKLAKELNIDVSGLYKLLKNKNSNPT
                     IDTLIKLADYLDITLDELVGR"
     gene            12996..13388
                     /locus_tag="PC429_001789"
     CDS             12996..13388
                     /locus_tag="PC429_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847297.1"
                     /translation="MDISESITKQFSDSLKSLIEIEINKQETDRVKSQTVEQKVKVLE
                     PKDIVVLIKRGYPNYLITVEEARGILKLDTVFMRRLVSTGLIKSLARGDGRKISRYEV
                     DDFIERNQGKNLDELLKVAERGDEIVKP"
     gene            13375..13518
                     /locus_tag="PC429_001790"
     CDS             13375..13518
                     /locus_tag="PC429_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847298.1"
                     /translation="MLSLNTNKNNIVTLKKDGKAIADIVFKDIKTGKKISVGVLNKKV
                     LVK"
     gene            13685..13927
                     /locus_tag="PC429_001791"
     CDS             13685..13927
                     /locus_tag="PC429_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847299.1"
                     /translation="MEAARLIAIGQIKQAEKEICKLQGTKNNSSLMWWEAVKFASQNI
                     LEGLEHDIELEASIEFREAMMYQEELEKDRPIDVQI"
     gene            14006..14287
                     /locus_tag="PC429_001792"
     CDS             14006..14287
                     /locus_tag="PC429_001792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847300.1"
                     /translation="MKTKNEIIKGLEDRLFLLRFTTVDEVDWDVKFGQISALEFCIDK
                     HRKGCTLEQFKEHLDEYKLQGNYGDYIDGFVSVLERNIREMEGEIDGSE"
     gene            14274..14912
                     /locus_tag="PC429_001793"
     CDS             14274..14912
                     /locus_tag="PC429_001793"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF3847301.1"
                     /translation="MEVNNIYIKLMDVRIKFNKLDIKKSGQNKFANFKYFELADFLPQ
                     ATELLQEAKLCPIVTFTNDYATLTLINGEKPTEEIIFTSPMRELQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDTFDATSGKEDYKRNDLTNSSIKASENGQIKISQNQIKRL
                     FSIGNTIGKDSDRVKSEVYYKFNKEVKDLSKQEYDQICVGYEKLQREKGIIK"
     gene            14922..15817
                     /locus_tag="PC429_001794"
                     /pseudo
     CDS             14922..15817
                     /locus_tag="PC429_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894692.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
     gene            15837..16256
                     /gene="ssb"
                     /locus_tag="PC429_001795"
     CDS             15837..16256
                     /gene="ssb"
                     /locus_tag="PC429_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF3847302.1"
                     /translation="MNQVVLVGRLTRDPELKYIPGTGTAVASFTIAVDRNYINKEGKR
                     DTDFIPIEVIGKSAEYCANYITKGKLVALEGNIRVDNYQTQSGEKRIFTKVSTKSVQS
                     LESKNKSSNSYKESVQDGTIGLDPQGFEIIDDDELPF"
     gene            16286..16648
                     /locus_tag="PC429_001796"
     CDS             16286..16648
                     /locus_tag="PC429_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847303.1"
                     /translation="MFKVERYFSGSVVDNLIEDDLTCRNYLALYCCLLGITKNGKKIY
                     PKPEKMLAEFGVKKDRKIKKELPVRIRNVNTGEVKEFESIDGAACFLRLKYQAVYQAI
                     KKKTKTRSGWKVEYIKEE"
     gene            16882..17733
                     /locus_tag="PC429_001797"
     CDS             16882..17733
                     /locus_tag="PC429_001797"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF3847304.1"
                     /translation="MNVLSLFDGISCGQVAFERARIKVENYFASEIKEIAIKVAMNNY
                     PKTIQLGDVRELNVSKIPKVDILIGGSPCQNLSKGRLIANKIQDGLTGDKSNLFWEYI
                     RILKQLNPEYFLLENVVMPKKDENMISQLLGVEPIKINSNLVSYQNRDRLYWTNILNV
                     QQPKDLKISFQDYKDTSFDYCKEFEIKKTPSREKMWGNGLNGNCKNVTYEDKINCVTL
                     KQDRFSNSGLVEFGSFCRYLTTRELELAQTLPVGYTKGLSIRQAQNVIGDGWTIDVIA
                     HILSNIN"
     gene            18050..18247
                     /locus_tag="PC429_001798"
     CDS             18050..18247
                     /locus_tag="PC429_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847305.1"
                     /translation="MNKFQKAVSQMVKQEEKENLLQGYENCIVGRSISSSIRRYVKGF
                     EKFGYSVHEVYEFINDINKYE"
     gene            18274..18690
                     /locus_tag="PC429_001799"
     CDS             18274..18690
                     /locus_tag="PC429_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDF3847306.1"
                     /translation="MSKYNNKKIVIDGIKFDSKDESEYYLYLKEKKENGEIKDFGLQQ
                     KFELQPKFKKDGKSYRAITYTIDFAIYKWNGEVVYIDVKGYSTQQGELRKKLFDYKYQ
                     DKKLIWIAKSKKYGVDGWIEYSELKKKRKENKKKVA"
     gene            18709..19209
                     /locus_tag="PC429_001800"
     CDS             18709..19209
                     /locus_tag="PC429_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847307.1"
                     /translation="MASKVKKEFFMATKKHLENYKQLHINIESLKLQIKNLKEFHLGD
                     FMQGLSYDSIPISKTNSISNQVENELINLEEKIIEKQIELYEMEALKYTIDVSISNLK
                     PIHKQIIRYRYIEGLEWSLIVDKVYLEERQLRERANQAISSISIALFGKKALIEQEPL
                     FKMLDL"
     gene            19631..19837
                     /locus_tag="PC429_001801"
     CDS             19631..19837
                     /locus_tag="PC429_001801"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847308.1"
                     /translation="MNRFEGIYSFAEATKLWDLKDSTLRKAVATGKLVENIDCKKFGR
                     DWVVTIEAMEREYGKLNNIMKDLD"
     gene            20326..21513
                     /locus_tag="PC429_001802"
     CDS             20326..21513
                     /locus_tag="PC429_001802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847309.1"
                     /translation="MLNEKNLKNIKNILQMVQMKHLKDFENWGINKRKKETAINSLIN
                     IIQNDGEKLDYFKEWISIFTIDGHNNYYVFNYNDSDLELEDLKNIVEDIVSENLLEID
                     TSDLEETKVIYKKNDVINKRLIIRFISPALISCKNENENGNIESGLEKTFYFATLFLD
                     LSLKQIIVSIPHTVGIKSIDNIEAKARDFSEIAKYYVSKLEDLLTTYNIQIEKYNDWI
                     YDATYELAEEGSAHNNPEINKKYEENIDKIDEFAKKIAIASGITDKAIIENFKEGVGI
                     LYENILIDEFGVVEDENQYSTFVQNGDGVNSYCRVGSKTATLKSGKGHAIAKTSRNNE
                     DVKNLGVMKSFNGTLSRFLIEVLDKELYLIRTDTSKFIEERVIYDVIRKVGEYKYTFR
                     KSE"
     gene            21458..21844
                     /locus_tag="PC429_001803"
     CDS             21458..21844
                     /locus_tag="PC429_001803"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847310.1"
                     /translation="MMLYEKLENISILLGNQSKAKSIIEELEKLMKSDIITPRLLARK
                     TKVTLLEATRVLEFLVKENELEFFIVVECSNPDSVYENEIGHYKYFTSIKEFNAFSKG
                     QECPICGCGYKYNFEDTKIGFRRLEK"
     gene            21849..22619
                     /locus_tag="PC429_001804"
     CDS             21849..22619
                     /locus_tag="PC429_001804"
                     /inference="COORDINATES: protein motif:HMM:NF016362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDF3847311.1"
                     /translation="MSLDTLKEYLSICDDNLIWEPFYDDSMLLDEFSNYIKEIELSKE
                     WNKSKNHEKGKILEQLVRFIMSRFTILSDISVNKSTTDNELDVFVKFNDNIPIPFLCN
                     IKSKIVCECKNWKSRKVDVGMVSKLAEICNKNNAGLGIFISLNGLTGKGWQYAEGKRR
                     KLYLSDKMPIISFTVDEITKLKYKRYNLLTMIKCKMQALIDEMEFAGGELRLLESELG
                     FIDCLNENIESLKMLGLITEVEYNNIKVRINHRYDIVN"
     gene            22677..23360
                     /locus_tag="PC429_001805"
     CDS             22677..23360
                     /locus_tag="PC429_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847312.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWANE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETQDLINEIKDKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKYESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDELKIS
                     VDYGDRNDS"
BASE COUNT         9410 a         2555 c         4021 g         7404 t
ORIGIN      
        1 tttatcacag ttaataaatt tattagtttg ttgtagctaa taaaaactga aaattctaaa
       61 attataattg taagattata taaatttaga atttaaatct gttgaataat attaaaacaa
      121 tgataatgag tatgaatttc taataaaaat aaatatcata aaaataagaa aaagtattgg
      181 gaggaaatga aaatggcaag aatgtattat gaaaaagatg tggatttaga ggttttaaaa
      241 aataaaaagg ttgcagtttt aggttatgga agtcaaggac atgcacatgc acaaaatctt
      301 agagataacg gtgtacatgt aatgataggt ctttatgacg gaagtaaatc agcacaaaaa
      361 gcaaaagaag atggatttga agttaagagt gtagcagaag ctacaaaaga aagtgatttg
      421 acaatgatgt taatgcctga tgaaaaacaa aagaaagttt atgaagagag tgtaaaggat
      481 aacttaaaag aaggtcagac attagctttt gctcatggat tcaatataca ttataatcaa
      541 gtacaaccac cagaatttgt tgatgttgta atggtagctc ctaaaggacc tggtcactta
      601 gttagaaatg tatttacaaa gggaagtgga gttcctgcac tatttgcagt atatcaagac
      661 cacactaaaa aagctacaga gacagtttta gcatatgcaa aagggatagg agcaacaaga
      721 gcaggtgttt tagaaactac ttttaaagaa gaaacagaaa cagatttatt tggagaacaa
      781 tcagtacttt gtggtggaat aagtgaactt ataaaattgg gatataaaac gcttgtagat
      841 gcaggttatc aaaaagaagt agcatatttt gaatgtttac atgaaatgaa actaatagtc
      901 gaccttatat atgaaggtgg atttgaaaga atgagatata gtataagtga cacagcagaa
      961 tatggagatt atgtatctgg taaaagagta ataactgatg ctgcaaaaca aggtatgcaa
     1021 aatgttttag aggatataca aaatggaaaa tttgcaaaag cgtggattaa agaaaatgaa
     1081 gaaggacgtg aaaacttcct aaaaactaga gaagaagaat ataatacaga aattgctgaa
     1141 gttggtagaa atctaagaag tatgatgtca ttcttaaaat aaatatatat taaatggatt
     1201 agtttgaggg aaagagtggt gtatagtagt gcgtatgaat ggagcaaagg taattttaga
     1261 atgtttaaag aaagagggaa ttgacacaat atttggatat cctggtggag ccgtgatacc
     1321 tctttatgat gctctatacg attattcaga tgattttaaa catataagga catcccatga
     1381 acaaggttta gttcatgctg cagatgggta cgcaagaagt actaatacag taggagtttg
     1441 ttttacaaca tctggtccag gagcaacaaa tgccataact ggaatagcaa cagcttttat
     1501 ggattcttca cctatggttg taatttcagg gcaggtgcca actagtttat taggaaaaga
     1561 ttcttttcaa gaaattgaca taactggagc aacattatct atgacaaaac ataattattt
     1621 agttagaaac actaaagaat tagttcctac aataaaagaa gcttttagag ttgcaaattc
     1681 aggtagaaaa ggacctgtac ttgtagatgt tccaaaagat ttatttttag cagaaatgga
     1741 ttttagtggt gaggactatg atttatgtca aatagatgat tatatggatt ataagagtga
     1801 ttttgatcta gatgatgaaa ctaatattaa gcttttaaat gaagcaatag atatcataaa
     1861 agaatctaaa aagcctgtaa tatatgcagg tggaggtgtt aaatcttcag acagtgagga
     1921 aattcttgaa aaatttgcaa ctaagataga tacacccgta ctaaatacac ttatggggct
     1981 tggaaatata gatagaaaga atgaactatc tcttggaatg gttggaatgc atggaagtag
     2041 agaaagtaat ttagcacttt ctaattcaga tttagtaata gcgataggtg cgagatttag
     2101 tgatagagtg ataagtaaaa gttctgaatt tgctaaaaat gcaaaaataa tacacataga
     2161 catagaccca tctgaaataa gtaaaaatat agaatctaat gtatctttgg ttggagatgt
     2221 aaagctagtc cttagtttat taattgaaag ggttgaaagt aagaataata gcaattggaa
     2281 agaagagata aagagattta gaaagagtga gggtgtacaa acatatgaat ttcatccaca
     2341 aaatatactt aaaaaaataa atgaaaaata tgaaacatta aagaaaccca ctgtggtagt
     2401 aactgatgtc ggtcaacatc aaatgtgggc tgctaagtac tggaatttta aaggtaataa
     2461 aagttttata acatcagcag ggttaggaac tatgggattt ggtttaggtg cagctattgg
     2521 aactaaagtt ggtaatgtag ataaaaatgt ggttttagtc actggtgatg gaagttttag
     2581 aatgaactgt aatgaactag ctacagttgc aaattataat gtacctatgc ttattttact
     2641 actcaataat agaacattag gaatggtaag gcaatggcaa aagttatttt ccaatcaaag
     2701 atactctcaa actgacatta atgaaaatgt ggattatgtt aaactcgtaa atgcatataa
     2761 tattgatgga tataaggtat ccagtataga agaattagga aaggcgctag atatgataga
     2821 ttttaataaa cctgtatttt tgcaatgtga tatagataag gattatgatg tttatcctat
     2881 tgtagcacct aatgatgcat tggaaaattt gatatgtaac taaaacttat actgaatatt
     2941 gataaaattt tttattaaat ttttattggt attcagtttt tgttgtttag agatagattg
     3001 gcatttttta ttttttcaaa taaatcaatg actatataat taagaactta ttaaatttta
     3061 attatacaat ttggaatagt attacaactt aagtaaatat taagttttat tcgttagatt
     3121 tattattaat accttatatt agttatggtt gttatatact ctaaagcctt gaaattacaa
     3181 gtgtatagct gactatataa atttgataat tttagctttt aaaatagata aattagattt
     3241 tgccacccat ttgccaccgt attatatttt tgggtggcaa actctaattt gtaatttttt
     3301 caaatatatc tacagtttca tttttcattt tatcagttac atgtgaatag gtatccattg
     3361 tagttgatag ttggctatga cccaaacggt tttgtatgtc tttaatgtta gcaccatttt
     3421 ctaataatag agtagcatgt gcatgtctta aagaatgaaa atggaagtca ttgtttaaag
     3481 ctactcgaat ttgtcttact atagtgtcta aagtgtgagt attcacttgt tgaccatttt
     3541 ctttggtaca tacccaatca ctatcaaagt aaaattctcc atattttaat ttcattttct
     3601 tttgatataa tttatgttct tttaatgcct ttattaaagt gtcacctgta aatatagttc
     3661 tgcaagaact ttctgttttt ggttgcccta attcaaacat tccatttggt tttttaatca
     3721 aagtatgctt tactgtgata gttttattat caaggtctat attatcccat tttagtgcaa
     3781 taatttcacc tcttcgcata ccagtatgaa atccaattag taaaactata cgttgaaatg
     3841 aatcttgagg aaatatattt agtatttgat taaattcttc taatgtaata gttttaactt
     3901 tattagtttc tgttttagat ttagtttttg gtatgcttac atattgcata gggttttctc
     3961 gtatgtgttt gtaaggatgg actgctgatt ttaatgacct atgtaatatg gcttttaata
     4021 cttgtaatgt attttgagag taatcatctt tgtacttttt atttatgaag ttttgtagta
     4081 ttgcaggagt taaagctttt actttgtaag ctcctagttt tggctttata tgtttttcta
     4141 tgtttattcg gtagctttct tgagtgttgt atttacagtt aagtaagaca tattctttgt
     4201 accaaaagtc taagtagtct gataaactga tattgctttc ttcaaatact atgccagagt
     4261 tttcatattc atttagtgct tctcttaagg ctttttctgc ttctttttta gtattgcctc
     4321 caactctttc tacttttttt ctttttcctt ctactatgcc taggtcaaag tagtaatacc
     4381 atttgttact tctttttctt actccacctt tcataatagt atccctccct tttagaatgt
     4441 atgtttgttt ggtgtttata taaaagagca gattaactgc tctttatata cttttgttat
     4501 gtacaatatt atcttaattt tgtttatctg gttttcactt aatcattttc ttaatattct
     4561 tacaattttt tagcttttat gtgtagttta ttataataat attgaaatat atatttatta
     4621 agtaaaaaat ctataaaagt agaataaatg agtttaaaaa atatcctcat atatgtagaa
     4681 atttggcaaa ttagttaata ctaaaaatct attatttcct aagtccaaca tgcttttctt
     4741 ttttgataga aattctaatc tttttagtaa gaagttgatg ctaacttgta attcttcagc
     4801 tatttcgtat acacttgtag cgtgtgaatt aataacatgt attatttctt cttctgttat
     4861 aagaaattca catgcccatt ttagtgcttt gttttcagtt ttgtctatca agattttatt
     4921 tttgtaactg ttttttgaag atacatagtt tccaacactg gtaaaatgat gtcctaattc
     4981 ttctgctaag atttctatta gtttagcatt gttttgtttt aatgaattaa gtaatgatat
     5041 aatctttagt ccttgtctgt ttatatacaa tccttttatg tcatctgcta ttttgtcagt
     5101 gtagtaaatt tctatctctt cattatttgc taagtctaaa agtgcgtcta gtttgttcat
     5161 tgaaatcccc ctataaaaag aatgtatttt tgatttttgt tatatataaa gagcaggtgt
     5221 gaactgctct aaatattcat tttttatcca aaaaagattg gataatttta gctgtagaca
     5281 aaatatggat tatagtatat gattataata taattacata taatatatgt aagcacttac
     5341 aaatgtgtgt tagtatagtt agcactaaat ttgtatttgt gcttaaagcg attaaaggag
     5401 ttttggagcg gtgggtagag ttttactcac tataaccaag actcttttgc atattagagt
     5461 atttcatgta gtcatatatt ctaataagcc ttcttaaatt tgttttttcg cttgtttttg
     5521 cagactctaa aatattagga ttttcctcaa atattttctt aagtagattt tctaactgtt
     5581 cagtgtcaat ttctttttct tctaaaaaag caataagtct tgtagctttt tcaaatgatt
     5641 gatgttcatc attccaaata ccataaacac ttctattttt tatgtaagta ttatttctat
     5701 tttttttaat actattgcta ataatatttt caaagtcact ttctagtgca attgtcctat
     5761 aatctggaaa ttcaagtgtg ctattaaaaa gatgtttatt taatggtaat ttattatatt
     5821 gcttaattaa agatggataa gcatattcaa gtaattcatc tgatgaaaat tctaagtcat
     5881 tcatgactat atcacgatac cattcatcaa aacttaatcc ttttaatcct ttaagaccaa
     5941 aatcacttgc cttgccaact gctaaaacta aatcttcatc ttttattctt gtatcatcaa
     6001 cactaccaac ccgaatttta gcagtaggtt tgttagtaat tgtgaaatcg taaaattctt
     6061 gtttgaacat tcttaatatt ctaactggta attttgattt tttttcagat aatgcctcat
     6121 agagtaaatt aaaatcctcc agtttaattt tagtcatagt aatcatttta ttatcaaaaa
     6181 caattgtatg acttgatatt tctatatcat taaagttttt ttcatactca atgaatatga
     6241 aacgttcctc gagtttttta gcattttcat tagatagaca attaactata gcattaagta
     6301 ttttttttat atttacatct gtaacagagt aaccaataaa tatgatagga aactcaacaa
     6361 atatagtcat tagcttagct gccaagtagg cacttttgct gtcaaagtct atataatcag
     6421 cttcatttat tacaatacta tttggagaag aaacacatcc atgaatttta taaatttctg
     6481 caataccttg tatagatgaa aaaactaaat tttcttgacc tatatatgtt gtataattat
     6541 caacgatagt ttccaaaaaa caatcataat ttgttgtaat aaatcctgat aaactttttt
     6601 tagagatatt actaagtttt tcaacttcat cttgatattt atctactatt ttagaattat
     6661 ttttaatata catagcaatt tctgccttaa atggagaaac tcctgattta acaatatcta
     6721 aatacttaga gtctaagctt cgtatagaag aatctgaaaa ccatttttta ttaaaatcat
     6781 tttcaataag ttctgcaatt ctaggattta tacccatttt tgggttctct gattttgctt
     6841 tatttttata acttgtatat ataaagtcat cattactaat tttttctgca aagactttta
     6901 gtaaatcctc ccaatttgga agattatagt atcttctagt taaaccagaa ccaacaaata
     6961 aaaatggtgt agcattaaat cggtttatga catctgatat tatagatttc atataattta
     7021 tcacctactt ttaatttatt ttttatactt atttattaaa aattcaatat aatcattgag
     7081 ttgttcttga gcttcctctg gtaaatcttt gtgaggatta actctatgtg cagctacagt
     7141 gtctatatca ttttttacaa tagacctacc taataaataa tcagtagtca cattaaatat
     7201 ttcagcaagg ctagatagca tataagcatc aggaactgtc tttccttgtt cataataacc
     7261 ataagcgctt ttggttaagt ttaatttatt tgcaacatct tcttgagtca tatttttttc
     7321 agctctaagt tcttttagtc ttttttgaaa taatgtatta ttacaagaag ttttgctaga
     7381 aatattgctt gctttattat catacctacc tattaaataa tcaacagtag tgccaaaata
     7441 ttcagctaaa ctagataaca tttctggggt aggtgttctt ttaccttgtt cataaaatcc
     7501 atatgcacta gtagttatgt taagatagtt ggcaatttct ttttgcatta cacctttttc
     7561 tttcctaaga aatttcaatc tatctgataa aatgctcata taatcactcc tcttaataaa
     7621 gttgccttta ttaaataata caacaaatag ttgtaaaatg aaataaaaaa ataaaaaaac
     7681 tatttacaaa caacatatag ttgtgtatta tataagtata aacaacaaaa tgttggtaag
     7741 gaggatatag cttgaacaat ctaaaggaac ttaggacaga aaggaattta aagcaaaaag
     7801 atgttgcatt aaaagtaggt ataactacta gttattatgg aatgattgaa agtggaacaa
     7861 gagtaccaag tttagcaata gcaataaaat tatctaaatg ttttggagta tcaatagaaa
     7921 aaattttttt aaatacaaat acaacaagat gttgtttaaa agagaggagt taaatatgaa
     7981 agatttaaaa atagtaaaag ttaataataa actaacaact gacagtagag atatagctct
     8041 aatggttgaa aaagaacaca agattttact aagggatata agaaattata taaaccaaat
     8101 ggaagaagcc aataaaaaca tgagtacaga tttgtaccca tctgattatt ttattgaaaa
     8161 tacttattta gatgattata aaagagagaa gccatgctat gcaataacaa agataggttg
     8221 tgacttcata gcaaataaaa tgacaggcat aaaaggtacg gcatttacag gaatatatac
     8281 aaaaaaattt gaagaaatgg agcaagtatt aaaaaatgaa caacctaaac taccaactac
     8341 atataaagaa gcattacaac atcttataga gcaagtagaa gtaaatgaga aattacaact
     8401 agaaggaaaa atgaaagacc aagtaataaa agaactaaaa ccaaaggcag attatacaga
     8461 tatgatactt aaaaacaaag gacttgtcac aataactcaa atagcaaaag actatggaat
     8521 gagtggaaaa gaaatgaata aaatacttca tgaaagaggg attcaatata aacaaagtgg
     8581 acaatggctt ttatataaac aacaccaagg gaagggatac actcattcag aaacaataga
     8641 cataactaga agtgatggaa tgactgatgt aaaaatgaca actaagtgga ctcaaaaggg
     8701 aagattgttt ttatatgact tattaaaagt aaataacata ttaccagata tagaaaaaga
     8761 gtatagttat caaacttcaa tgttatgtta gtactttgaa aaataaatac agaatattca
     8821 aaaagaggtg atttaaaggt tgagtaatag aaagaagtat accttgagta ttacgaaaga
     8881 attatacaat aaatgtaaag aaaaagctaa tcaaagaggt atgtcagtga atgattacat
     8941 actatcagtg attagcaaga gtatataaat tattcttcaa tattaatttt aatttctcct
     9001 tttctttgct cataatcttc aatatgtttt tttattattt gttcaatttc cttattagca
     9061 gaacgacctt cgctttcagc tatatattta attttttcaa gcagaactct gtttattctt
     9121 aaagtgtatt taggtagtga agttgacata ataatacctc ctttttaaag tctaaatgat
     9181 gtcataatta tatcataaaa ataatttata gaaaatctat aaaatatatt gacggcataa
     9241 agacggcgtg atattattaa aacaagaaag gggttgacgg caaaatgaac tcaaataaaa
     9301 agagaataac tgttaggatg ccagaaaaat taaatgaaga aataacgaaa aaatcaaaat
     9361 acttagggtt aactaagaac tcatttatat tagatatact atggaaggaa tttgagttat
     9421 tagaatatag aagttataaa aaggaggtag ataaacatga ataacttaca agtaatagaa
     9481 agaaataatg agagagttct aactacacaa caattagcag atgtatatga aactgactca
     9541 agaaatataa gtaacaattt caacaataac aaagataagt ttattgaagg taaacattat
     9601 tttttattgc aaggtgatga tttaaagaat tttaaaggca ttcatacaga atatgaaaac
     9661 ctaaaatttg cttcaaaaat gtatctttgg actgaaagag gagcaaatag acactgcaaa
     9721 atattagata cagataaggc ttgggaacag tttgataatt tagaggaaac atatttcaaa
     9781 gttaagcaac ataagccaac ttgtatagaa gatgtattaa tagaaagttt aaaagaaatg
     9841 aaagatttaa gacttcaagt taatcaagca aatagcattg ctttagaagc aaagacagag
     9901 gttgaaacaa taaaagatgt agtttcatta gactcaaata gttggagaac aaatacacat
     9961 caactaattg caagaatagc aaaaaaacaa ggtggttttg aacatataaa tatgcttaga
    10021 acagaaagtt atgaattatt aaataagaga tttggagttg acctacatag aagattgatc
    10081 aataaaagaa gaaaaatggc agaagaaggt gtatctgaat ctaaaagaga gaaagttaac
    10141 aatttagatg taatacaaga tgataagaaa ctcatagaag ggtatgttgc tattgtaaaa
    10201 gatatggctt taaaatatgg aatatcaagt gatttaagca aaaattaagt taaatcacaa
    10261 cagcactttg aaaactaaat ataaaataaa aatatctccc ttaaaagttt tttaaagaga
    10321 gataaacact ttattttgta actatttgaa tacatgatgg atgaatgcaa tagtttttat
    10381 ctttgtaatt tatagaaaca taattactag agtacatttt atcaaggttt tcattaatat
    10441 attcaattgc attacagcca tttatagagc caatatcagt tatatcaatc cagttattaa
    10501 atatcttaac ataaacaaac ttcatgataa caccaacttt cattagaatt taagttaatt
    10561 ataacatggt gtactgagat aatgtgttaa gaaatagata cagaatattc aaaagaggtg
    10621 attagatgga aatagagcaa acaacaatac gcctgctaaa aagcagacga aagttgaata
    10681 ttaatttcta ttttggttag gcgaaagacc taacaaaaaa tctgtagata cattaagttt
    10741 gttagaaatt aatattaaag tttctagatt tggttctctt gaaccagctt cataatactg
    10801 atatgcacgt tctgagatac caaacaaact agcaaattgc ttttgagtca tattcatttg
    10861 ctttcttact agcttaatat tatctttgaa tttagtcata actacacgct ccaataaaaa
    10921 aataaataaa tttcttgaca cgaacaatat tagcgtgtaa tatataaaac atagcacgaa
    10981 caatattggc gtgttaaata aaaaggagag ggtgaaaata aataataatt taaaactaca
    11041 acgtgaaaaa attggcttaa cacaattaga ggttgctcaa aaagccaaaa taacagagag
    11101 aagctatcaa tattatgaag ctggcgaacg tctcccaaac attcgtacag ctttaaagat
    11161 agctataatt ttaaatacta attgtgaaaa actctttaat gaataatagc ataaaaggac
    11221 tcagattaca agaaaggagc tttagatatg aataatttgc aacttaacaa taaaaataca
    11281 ataacaacat tagaaattgc tgatatgtta gaaatcagac actgggaagt cttaaggaaa
    11341 ttagaaggaa cagaaaaaac aaaaggaatt attgatattc ttaacgacaa caattttgtt
    11401 gtggttgatt actttataaa atcaacatat ttggattcaa aaaatgaaag tagaccttgc
    11461 tataatgtaa caaaattagg ttgtgacttt ctagcaaata aatttactgg agaaaaaggt
    11521 attattttta cagctagata tgtaagaaga tttaatgaaa tggaacgagt attaaaagaa
    11581 caacaaccta aactaccaac tacatataaa gaagcattgc aacagttatt agtagaagtt
    11641 gaagaaaaag aacaattaca attagaaaat caagaaaaag ataaggtaat tcagttacag
    11701 caaccaaaag tactgtttgc tgatgcagtt tcagcttcaa acaattctat attagttgga
    11761 gaacttgcaa aacttataaa acaaaatgga gtagatatag gacaaaacag attatttacc
    11821 tggatgcgag aaaatggata tttaattaag cgtaaaggtg aagattataa tattccaaca
    11881 cagaaaagta tgaatctaag aataatggaa gttaaaaaaa gagtaattaa taatcctgat
    11941 ggaagtacta aagtaactag aacagtaaaa ataactggaa aaggtcaagt ttattttgtt
    12001 aataagttta aaagtagtaa acaattatca atgttaagtt aaatcataac agtaccttga
    12061 aaactaaata cagaatattt tggaaaggag agattgaatt ggaaaataag aaaatagaag
    12121 aaatagagaa aagaataacc atgcttgaaa atcaattgca agcacagtta tttagtcaga
    12181 taacagctca atcaaatagt gattttatag attatatgta ccaatgtata gttttgtatt
    12241 tacatgagat gaataaaagt gaaaaaagaa aaattagtga aattactaaa gaaagaatca
    12301 gaaaataaga atgataatta caatataaaa cctattcttc tagcaggttt tgaagggtca
    12361 gttttcttta ctgaagttat cataaagttt atctgtgatg tgtgttgaag aatttcagaa
    12421 taaactccat ttactatgcc gtagtagtga attagacaag ggttatgata acctacttct
    12481 tcaacaatca ttaatacatc ttttccaaag ttagtcaatt ttagtgcaat ttcatgttca
    12541 tcatcaagtt ctttttcaaa ttcctctata gatttaagaa taattttata ttgaaattca
    12601 gcatagtaaa aatactctgt gttagaagta tcagagactg tctttcttat actttcttgt
    12661 aattctttag atatattgtt tgaaaactgc ataatacacc atccttttga aaattttgga
    12721 atttattcca tgtttatatt ataccatgta gaactgaggt gaatacaatg ttaataggcg
    12781 acaatataaa gcaaatacta agaaaaagag atataaaacc ttataaatta gcaaaggaat
    12841 taaatataga tgtaagtggt ctatataaat tgttgaagaa taaaaattct aatccaacta
    12901 tagatactct aataaaatta gctgattatt tagatattac attagacgaa ttagttggaa
    12961 gataaatatt tgaaaagagg ggagagaatt ttaacatgga tatttctgaa agtataacaa
    13021 agcaatttag tgatagttta aaaagtttaa tagaaataga gataaataaa caagaaacag
    13081 atagagttaa gagtcaaact gttgaacaaa aggtaaaagt actggagcct aaagatatag
    13141 ttgttttaat aaaaagaggt tatccaaatt atttgataac agtagaagaa gcaaggggaa
    13201 ttttaaaatt agatacagtt tttatgcgta ggttagtgag cacaggtttg ataaaatcac
    13261 tggctagagg tgatggtaga aaaatttcaa gatatgaagt tgatgatttt attgaaagaa
    13321 atcaaggtaa aaatttggat gaacttttaa aagtagcaga gaggggggat gaaattgtta
    13381 agccttaata ctaataagaa taatatagta actcttaaaa aagatggaaa agctatagca
    13441 gacatagtat ttaaagatat taaaactggt aagaaaatat cggttggagt attaaataaa
    13501 aaagtgctgg tcaaataacc aacacacaaa aaaataaaaa ataaaatata acacaaacaa
    13561 attatagcac aaaaagaatg gatttagaat aggtaatata acattctaaa aggtttaatg
    13621 tgtaataatt gtaacaattt agaatgtagc ttatatagat agtaataaat tgggagggat
    13681 ttaaatggaa gcagctagac taatagcaat aggtcaaatt aaacaagcag aaaaagaaat
    13741 atgtaaatta caaggtacaa aaaataatag tagtttaatg tggtgggaag ccgtaaaatt
    13801 tgctagtcaa aatatattag agggtcttga acatgacatt gagttagaag cttctattga
    13861 gtttagagaa gctatgatgt atcaagaaga acttgaaaaa gatagaccaa tagatgtcca
    13921 gatataaaaa aagagccttc gcacaggctc taagtgaaaa taagttacaa aaattatagg
    13981 tatattataa cataaggggg aataaatgaa aacaaaaaat gaaataatta agggtttaga
    14041 agatagatta tttttattaa gatttacaac agtagatgaa gtagattggg atgtaaaatt
    14101 tggacaaata tcagcattag aattttgtat agataaacat agaaaaggat gcactttgga
    14161 acaattcaaa gaacatttag acgaatacaa attacaaggg aactatggtg attatataga
    14221 tggttttgtg tcagttttag aaagaaatat tagagaaatg gagggagaaa ttgatggaag
    14281 tgaataatat ttacataaaa ttgatggatg taagaattaa gtttaacaaa ttagatataa
    14341 aaaagagtgg tcaaaacaag tttgctaact ttaaatattt tgagttagca gacttcttac
    14401 ctcaagcaac agagctgtta caagaagcta aattatgccc tatagtgacc tttacaaatg
    14461 attatgcaac tctaacattg attaatggag agaaaccaac agaagagata atatttactt
    14521 ctccaatgag agaattacaa ttaaaaggct ctaatgagtt acaagcatta ggaggaattg
    14581 aaacatatca gacaaggtat ttgtacattc agttattaaa cattacagaa aatgatactt
    14641 ttgatgctac tagtggaaaa gaagattata aaagaaatga cttaacaaac tcctctatta
    14701 aagcaagtga aaatggtcaa ataaaaataa gtcaaaatca aataaaaaga ttgttttcaa
    14761 taggaaatac aataggcaaa gattcagata gagtaaaaag tgaagtgtat tataagttta
    14821 ataaagaagt taaagattta agtaaacagg aatatgacca gatatgtgtt ggatatgaga
    14881 aattacaaag ggaaaaagga ataattaagt aggtgaattt cttgaaggac agagacaaag
    14941 caactataga gaaaggaaat atacttagtg atgggtatgg tctttcacca caattggtag
    15001 ctagagattc atggctaaca acaggagcaa gagctttata tttctatcta tccagttttg
    15061 caggagcaag tgggacatgt tatccatcta gggatattat gactcatgaa cttggtataa
    15121 ataaagatac ttttagtaaa tacctaaatg aactaaagac gagtggttat ataaaagtat
    15181 ataaaaataa aactagggaa ggaaggatgc aaaataatat atatgaggta gtatttgata
    15241 gaagctatat agaaagtcat atatccatta gatgtaagaa agaaaataaa aaaaaccatg
    15301 tccgaaaaaa gcagacatgg aaccatgtcc gaatttaccg gacatggttc aaccggacat
    15361 ggaaaagtcg gacactataa gtaacagtat tataaataac agttttaaaa gtagtatgta
    15421 tatagagcaa gctgtggata actctttaaa agaatttaag aagctatatg aagaaaatat
    15481 aggagtagta tatccagtca cagctgaatg gttattagaa gtatctaatg aagtagatat
    15541 aagagtattt aaaagagcta tagagatatg tgctgaaaga atgaatatga atttatcata
    15601 cttaaaagga atccttaaaa agtggaagga tgcaaatata actacatatg aacagttaga
    15661 atcatataaa ttacaacatg aaaataaaaa gtcaaaaaaa accaatagtg tagtaagcaa
    15721 aaataagttt gctaattttg aacaaacatt tactcaatac agtaacaaag aattagatga
    15781 aattataaag aaaagccaaa aggctaaatt taaataatat tgatggaggt attaaaatga
    15841 atcaagttgt attagttgga agattaacta gagacccaga actcaaatac ataccaggaa
    15901 caggtacagc agtagcatca tttacaatag ctgtagacag aaattatata aataaagaag
    15961 gaaaaaggga tactgatttt ataccaatag aagtaatagg taaatcagct gaatactgtg
    16021 caaattacat aacaaaaggg aagctagtag cattagaagg gaatataaga gttgacaatt
    16081 atcaaactca atcaggtgaa aaaagaatat ttacaaaagt cagtacaaaa tcagtacaat
    16141 cattagaaag caagaataaa tcgagtaatt catataaaga gagtgtacaa gatggaacca
    16201 taggactaga ccctcaagga tttgaaatta tagatgatga tgagttacca ttttaatctg
    16261 aaaaattaaa tatgaggtga aataaatgtt taaagtagaa aggtatttta gtggctcagt
    16321 agtggacaac cttattgaag atgaccttac atgtagaaac tacttagcat tatattgttg
    16381 tttgttggga attacaaaaa atggaaaaaa gatatatcct aagccagaaa aaatgttagc
    16441 tgagtttgga gtaaagaagg acagaaaaat aaaaaaagag ttaccagtaa gaattagaaa
    16501 tgttaataca ggggaagtaa aagaatttga gtctatagat ggtgcagcct gttttttaag
    16561 attaaaatat caagcagttt atcaagctat taaaaagaaa actaaaacaa gaagtggctg
    16621 gaaagttgaa tacattaagg aggaataatg gaagtttcaa ggacagaata tacaattaaa
    16681 agagcaaaag agttatatga caatggagag gatatattta ttgctataga taaggctaga
    16741 gaaaaatatg aggagatgat taaaagtgaa tatcttagct agtgtgatat tagtaatagg
    16801 aagttttata gctggtagag tttatgagta tagattgaac ctaaaagggt gtgaaaattg
    16861 tgataataag aaggtgcaaa tatgaatgta ttaagtctat tcgatggaat tagttgtggt
    16921 caagttgctt ttgagagagc tagaataaaa gtagaaaatt attttgcaag tgaaataaaa
    16981 gagattgcga ttaaagtagc aatgaataat tacccaaaaa caatacaact tggagatgta
    17041 agagagctaa atgtaagtaa aatccctaaa gtagatattc ttattggcgg aagcccatgc
    17101 caaaatttaa gtaaaggaag attaatagct aataaaatac aggatggatt aactggagat
    17161 aaaagtaatt tgttttggga atatataaga attctaaaac aattgaatcc agaatatttt
    17221 cttttagaaa acgtagtgat gcccaaaaaa gatgaaaaca tgataagcca attgttagga
    17281 gttgaaccaa tcaagataaa tagcaattta gttagttatc aaaatagaga cagattatat
    17341 tggacaaata ttttaaatgt tcaacaacct aaagatttaa aaattagttt tcaagattat
    17401 aaagatacta gttttgatta ttgcaaagag tttgaaatta aaaaaactcc aagtagagaa
    17461 aaaatgtggg gtaatggttt aaatggaaat tgtaagaatg taacatatga agacaaaata
    17521 aactgcgtaa cattaaaaca agatagattt agtaattcag gtttagtaga atttgggtct
    17581 ttctgcagat acttaacaac tagagaatta gagttagcgc agacgctacc agttggatat
    17641 acaaaaggtt tgagtataag acaagcacaa aatgtcatag gtgatggttg gacaatagat
    17701 gtgatagcac atattttatc aaatattaac tagttcttta tatataaatt attattgaag
    17761 cggttagcta tcatgatact tgtagcagta agttttatag ctggtagggt ttatgagtat
    17821 agattgaatc tgaaagagtg tgaaaattgt aataacaact atcctgaaaa atgagaaaga
    17881 agtggtttta tgaataaaag aataatttgc aattggtgtg gtaaattatt ttacatccca
    17941 aaacagtcta aaaaaattta ctgttgtaaa agatgtgaga gaaaggctaa gaaaagcaat
    18001 agagaacagc aaaattaatt ttaaacaata aaactatggg ggaatagcaa tgaataagtt
    18061 tcaaaaagca gtttctcaaa tggtgaagca agaggaaaaa gaaaacttgt tgcaaggata
    18121 tgagaattgt atagtaggta gaagtatatc aagttccata agaagatatg taaaagggtt
    18181 tgaaaagttt ggatatagtg tacatgaggt ttatgaattt ataaacgata ttaataagta
    18241 tgagtaactt taagtgataa aggaagtatt tgattgagta aatacaataa taagaaaatt
    18301 gtaatagatg gaattaaatt tgatagtaaa gatgagtcag agtattattt atatttaaaa
    18361 gaaaaaaagg aaaatggaga aataaaagac tttggacttc aacaaaagtt tgaactacaa
    18421 cctaaattta aaaaagatgg gaaaagctat agagctatta catatacaat tgattttgcc
    18481 atatacaaat ggaatggtga agtcgtttat atagatgtga aagggtatag tacacagcag
    18541 ggtgaactta gaaaaaagct ttttgactat aaatatcagg acaaaaaatt gatatggatt
    18601 gctaaaagca aaaaatatgg ggtagatggt tggatagaat atagtgaact taagaaaaag
    18661 agaaaagaaa ataagaaaaa ggtagcttaa ataaatagga gtgatgttat ggcaagtaaa
    18721 gttaaaaagg agttttttat ggcaactaaa aaacaccttg agaactacaa acaactacat
    18781 attaatattg aaagtctaaa acttcaaata aaaaatctca aagagttcca tttaggtgat
    18841 tttatgcaag gtttaagcta tgacagcatt cccataagta agactaattc aataagtaat
    18901 caagttgaaa atgagttaat taatcttgaa gaaaagataa tagaaaagca gatagaatta
    18961 tatgaaatgg aagcactaaa atatacaata gatgtatcca taagtaattt aaaacctata
    19021 cataaacaaa ttataaggta taggtatatt gaaggcttag aatggagttt aatagttgat
    19081 aaagtatact tagaagaaag acaattaaga gaaagagcta atcaagccat tagttcaata
    19141 tcaatagcct tatttggtaa aaaagcatta atagagcaag aaccattatt taagatgtta
    19201 gatttatagg cagttaataa ctgtctattt tttttgtgaa aaagatatat ggaagttatt
    19261 gacaatatca cgcatacgtg atattattga agtatagaaa ggaggcgaaa agaaagtggc
    19321 taagaaaata aaagagttca gaaaactcat aaaagaatta actgaactcg tactcgaagt
    19381 tggcacacta ttagcagtca tcaaaatggt aatagatagc ctacattagt aaaatgttaa
    19441 gagtggaagt tgcaccttcc ctcttaacta aattataaaa catagtcact aaaaatacaa
    19501 tgaataagta tagagagtta acatttgaat tattgaaact actgtatgac actattaaat
    19561 taattggagc atcaatattg ttatattatg ttattaactc aattttctag caatgattag
    19621 gaggtatttt atgaatcgat ttgaaggtat ttatagcttt gcagaagcaa caaaattatg
    19681 ggatttgaaa gattctacac ttagaaaagc agttgcgaca ggcaaattag ttgaaaatat
    19741 tgattgtaaa aagtttggtc gtgattgggt agtaactatt gaagcaatgg aaagagaata
    19801 tggaaaatta aataatataa tgaaagacct agattaaatt ctaggtcttt ttattgccgc
    19861 ttttctgccg attttacaat ttaaaatgtg agataatagt attgtggaaa tgaatatttc
    19921 tctctcaaaa ctaaatatat gtgggctagg ttaagggatt cgcctagctt atatgaacaa
    19981 actaggcagg gcatgaggat gctgtaagtt caattcttac tatgttcaaa cttattaata
    20041 cactatatgt agatatgctg gattaaaacg gaatttaatt caaatgtcta aaagagtggg
    20101 gcttggtaac ctcactcaat ttgcaaggac tggtgtgtaa tcttaggttc gattcctaaa
    20161 acttgctccc ttaaatataa tatgtatccc caaaaaaagg cttagattaa attctaagtc
    20221 tttttttaat aattaatgta gataataata tttattgcta aaatgatgta caaatatgat
    20281 aatataaatt acataaagat atataaatag agggggatta taaatttgtt aaatgaaaag
    20341 aatctaaaga acataaagaa tatattacaa atggtacaaa tgaaacacct taaagatttt
    20401 gaaaattggg gaattaataa acgaaaaaaa gaaactgcta taaatagctt gataaacata
    20461 atacaaaatg atggagaaaa actagattat tttaaggaat ggatatcaat ctttacaata
    20521 gatggtcata ataattatta tgtttttaat tacaatgatt ctgacttgga attggaagat
    20581 ttaaaaaaca tagtagaaga tatagtaagt gaaaatttat tggagattga tactagtgat
    20641 ttagaagaaa ctaaggtcat atataaaaaa aatgatgtca taaacaaaag attgattata
    20701 agatttattt ctccagcttt aattagctgt aaaaatgaaa atgagaatgg gaatatagaa
    20761 tctggattag aaaaaacttt ttattttgca actttatttc tagacttaag tttgaaacaa
    20821 ataatagttt ctataccaca tactgtaggt ataaaaagca tagataatat agaggctaaa
    20881 gcaagagatt tctcagaaat agcaaaatat tatgtatcaa aattagaaga tttgttaact
    20941 acttataata tacaaataga aaaatacaat gattggatat atgatgctac atatgagctt
    21001 gctgaggaag gcagtgctca taataatcct gagataaaca aaaaatatga agaaaatatt
    21061 gataaaatag atgaattcgc aaaaaagata gctattgctt ctggaataac tgataaggct
    21121 ataatagaaa atttcaaaga gggagtggga atactctatg agaatattct aatagacgaa
    21181 tttggtgtgg tagaggatga aaatcaatac agtacttttg tgcaaaatgg tgatggagta
    21241 aattcatact gtagagttgg gtctaaaaca gcaaccctaa aatcaggaaa gggacatgct
    21301 atagccaaga catctagaaa caatgaggat gtcaagaatt taggtgtcat gaaatcattc
    21361 aatggaactt tatccagatt tttaattgaa gtcttagata aagaattata tttaataaga
    21421 acagacactt ctaaatttat agaagagagg gtgatttatg atgttatacg aaaagttgga
    21481 gaatataagt atacttttag gaaatcagag taaagcaaaa tctataatag aagaattaga
    21541 gaaattaatg aagagtgata tcattacacc aagattgctg gccaggaaaa ctaaagttac
    21601 acttttagag gctacaagag ttcttgaatt tttagttaaa gaaaatgaat tagaattttt
    21661 tattgttgtt gaatgttcta atccagatag cgtatatgaa aatgaaatag gtcactataa
    21721 atacttcact tcgataaaag aattcaatgc tttttcaaaa ggacaagaat gcccaatatg
    21781 tggatgtggt tataaataca actttgaaga tacaaaaata ggctttagaa gattggagaa
    21841 gtgatacagt gagtctagat accttaaaag aatatctaag catatgtgat gataatttaa
    21901 tttgggagcc tttttatgat gacagtatgc tattagacga attctctaat tatataaaag
    21961 aaatagaatt atctaaggaa tggaataaat ctaaaaatca tgagaaagga aaaattttag
    22021 aacaattagt aagatttata atgtctagat ttacaatatt aagtgatata tctgtaaata
    22081 agtctacaac agataatgag ctagatgttt ttgttaagtt taatgacaac ataccaatac
    22141 cattcttatg taatataaaa tcaaaaatag tatgtgaatg taaaaactgg aaaagtagaa
    22201 aagtggatgt aggaatggta tctaaactag cagaaatatg caataaaaat aatgcgggat
    22261 tgggcatatt tatttcttta aatggattga caggtaaggg gtggcaatat gctgaaggaa
    22321 aaagaagaaa attatattta tcagataaga tgccaataat atcgtttaca gtagatgaaa
    22381 taacgaaatt aaagtataaa aggtataatc ttctgactat gataaaatgt aaaatgcaag
    22441 cattaataga tgagatggaa tttgctggag gagaattaag attattagaa agtgaacttg
    22501 gatttataga ctgtttaaat gaaaatatag aatctttgaa aatgttgggt ttaataaccg
    22561 aagttgaata taacaatatt aaagtaagaa tcaatcatag atatgatatt gtaaactgag
    22621 aactccaaac gagttctttt tttattccca aaacgacaaa caaacgaggt ggtggtgtgc
    22681 aagatgtgaa agaaaaagta aaacaagatt acctaaaagg tatgaaacaa aaggaaatat
    22741 cagcaaagta tgacattagt ttaaatactt taaagtcatg gataaaaaga tacaattggg
    22801 ctaatgagaa aaagaagggt gcacctaaaa ataaaagagg tgcacccata ggtaataaaa
    22861 atgccactgg tcctcctgga aataagaatg ctgaaaaatt tggtttcttc tcaaaatact
    22921 tacctgaaga aacccaagac ttaattaatg agataaagga taaagataaa tttgatattc
    22981 tttgggaaca gataacaata caatatgcag caataataag agcacaaaag ataatgtatg
    23041 ttaaagacaa ggaagaaatg attaaggaat taaagaaata tgaaagcaca gaaaatggtg
    23101 agaagataga gtatgaattt caatttgcat gggataggca agcatctttt cttaatgcac
    23161 agagtagagc aatgagtgaa cttagaagtt taattaaaca gtatgatgaa atgattcata
    23221 aggattggaa tttggctaca gaggagcaga aaacaagagt tgagaagttg aaatgtgaag
    23281 ttgataacct aagtaaagat gatattggag atgatgagtt gaaaataagt gtagattatg
    23341 gtgatagaaa tgatagttag aatagatttt aatccagatt tcaaggaagc
//
LOCUS       DAMKMI010000049        23175 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMKMI010000049 DAMKMI010000000
VERSION     DAMKMI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.050"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            145..1377
                     /locus_tag="PC429_001806"
     CDS             145..1377
                     /locus_tag="PC429_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF3847313.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1391..2197
                     /locus_tag="PC429_001807"
     CDS             1391..2197
                     /locus_tag="PC429_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF3847314.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2309..3178)
                     /locus_tag="PC429_001808"
     CDS             complement(2309..3178)
                     /locus_tag="PC429_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3847315.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3550..4977
                     /locus_tag="PC429_001809"
     CDS             3550..4977
                     /locus_tag="PC429_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF3847316.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIGSVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5054..5323)
                     /locus_tag="PC429_001810"
     CDS             complement(5054..5323)
                     /locus_tag="PC429_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847317.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5326..6768)
                     /gene="nhaC"
                     /locus_tag="PC429_001811"
     CDS             complement(5326..6768)
                     /gene="nhaC"
                     /locus_tag="PC429_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF3847318.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6793..7959)
                     /locus_tag="PC429_001812"
     CDS             complement(6793..7959)
                     /locus_tag="PC429_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3847319.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8184..10304)
                     /locus_tag="PC429_001813"
     CDS             complement(8184..10304)
                     /locus_tag="PC429_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3847320.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10537..10938
                     /locus_tag="PC429_001814"
     CDS             10537..10938
                     /locus_tag="PC429_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847321.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            10981..11274
                     /locus_tag="PC429_001815"
     CDS             10981..11274
                     /locus_tag="PC429_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF3847322.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11289..11729
                     /locus_tag="PC429_001816"
     CDS             11289..11729
                     /locus_tag="PC429_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF3847323.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11821..12294)
                     /locus_tag="PC429_001817"
     CDS             complement(11821..12294)
                     /locus_tag="PC429_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF3847324.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12294..13082)
                     /locus_tag="PC429_001818"
     CDS             complement(12294..13082)
                     /locus_tag="PC429_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF3847325.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13410..14654
                     /locus_tag="PC429_001819"
     CDS             13410..14654
                     /locus_tag="PC429_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF3847326.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            14977..16074
                     /locus_tag="PC429_001820"
     CDS             14977..16074
                     /locus_tag="PC429_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF3847327.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGGLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16065..16763
                     /locus_tag="PC429_001821"
     CDS             16065..16763
                     /locus_tag="PC429_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3847328.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16766..17830
                     /locus_tag="PC429_001822"
     CDS             16766..17830
                     /locus_tag="PC429_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF3847329.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITSMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18013..18717
                     /locus_tag="PC429_001823"
     CDS             18013..18717
                     /locus_tag="PC429_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847330.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18710..20032
                     /locus_tag="PC429_001824"
     CDS             18710..20032
                     /locus_tag="PC429_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3847331.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20219..21592
                     /locus_tag="PC429_001825"
     CDS             20219..21592
                     /locus_tag="PC429_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847332.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEAFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(21964..22902)
                     /gene="pdaA"
                     /locus_tag="PC429_001826"
     CDS             complement(21964..22902)
                     /gene="pdaA"
                     /locus_tag="PC429_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF3847333.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8528 a         2887 c         3674 g         8086 t
ORIGIN      
        1 attatatcaa attgataaca aactagatat tatacttttt tgtagaatag aattacaatt
       61 atataaagaa aaattctatt aatagtgaaa aaaaagtaga aaaaagtaga aattattctt
      121 ttataaagaa agggagggat aatattgcaa gagagagaag aattagtttt aaatccagtt
      181 ccaaaagagg aaagagttgg atggttagct ccattgttta atatgcttgg ttctaacata
      241 gctatatctg aactaatggt tggaggaact ttaattttag gaatgactct ttctaatatg
      301 attattacat caattatagg gaacttaata ttagttgcaa ttattatgat acaaggttat
      361 ataggttaca aagaagggtt aaatacgtat gtattagcta agggggcatt tggagagata
      421 ggaggaaagt atcttatatc tctattactt ggtataacta gttttggatg gtttggtgta
      481 caagcagggg ttgcaggact atcagtacaa aagatatttc caagtgtaaa tctaacatta
      541 gttacagtta tacttggatt atttatggtt gtttttgcac tgtatggctt taaagctatg
      601 gctaaattta attacttagt tataccacca cttatgatat taatggtgtg gggagttttt
      661 aaagcttttt ctacttatgg agttgaagct atttataatt atactcctca aacaactatg
      721 agtatggttg aaggtcttaa tattgtagta ggtcttgtta tagttggtgc aattatatca
      781 cctgaccagt taagatatac gagacgagta aaagatattt ggattatcag ttttttaggt
      841 ctagggatta tttcattatt ccaacaagtt gcagctggtg taatgtctat gggagcacct
      901 acttgggata taacagaagt attggctaat ttaggttttg gatgggtagc gtttgttatt
      961 ttaatacttg catcttggtc tacaaacgta tcaaatgctt attctggtgg attagcatta
     1021 aaaactattt ttccaaatgt aaaaagaaat gttttgactt tagttgcagg tctaataggt
     1081 acaataatag cagctactgg tataatattt aaatttcagt catttttatc tttcttggga
     1141 atcgcagttc ctgcaatagc tggaattatg tggtgtgagt attattttat acaaggaaga
     1201 acttataagc atagagaagg aataaattgg attgcagtaa taagctggtt aataggtttt
     1261 gcagcgtcgt attatagttc aaaaataaat ttcttaatac ctccgataaa tggaattgta
     1321 gtgagtatgg ttatttatta tatattaatg aaatgttttg gaatcaaaga taaataaagg
     1381 gggcaattac atgggtcgta aggtgaaaat aggtataatt caacaacaca gtgtacttgg
     1441 agatgttaaa aaaaatatag aaaaggcagt agagatgata gatgacttag gaaaacaagg
     1501 ggcagatata atttgtttac cagaactatt tgctacagga tataatttag agtctttagg
     1561 tggagttaaa acattagaat taataagaga acataataag tacatagagg aatcaatgtc
     1621 agaagctgct aaaagaaata atgtatacct gatatctcca tatggaacct tagaaaaagg
     1681 gtctacacat gtttataatt cagcagttat atttgataga aaaggtaaga ttatgggaga
     1741 atactgtaaa aatcatctgt ggtcattaga agcagtatac tttaaaggag gggaaaaagt
     1801 agaggtatat gatgctgatt ttggtagatt tggtgtaatg atatgttatg atgcaggttt
     1861 tccagaagta tccagagaat tgacattaaa aggttctgaa ataatattta taccatctgc
     1921 atggagaata caagatgaag atatgtggga tttaaatgta tctcaaagag ctttagagaa
     1981 cactgtatat acagtaggtg taaatttagt aagtaatgac tctaatttaa tactatttgg
     2041 aaagagtaaa atatgcaatc ctagaggtac tgtaataact caattagata catacaggga
     2101 aaaatatgta ttatcagaga ttgatttaga tgaaataaaa aagtataggg aagatatccc
     2161 ctatttgaaa gacagaaaaa gtagctataa catataattc tatactataa tacaagttca
     2221 atataaaatg ataaaaagag gatgtatcaa tattaagatt aaatttatta tactttgata
     2281 tatcctcttt aatattatca actttgattt aaatattttt aaaatagttg ataagaaagt
     2341 ttttgaacaa caacacagct ttagattcat agttttcgct aaaccatgaa agatttaaat
     2401 atcttttaaa ttcaatatct ttgatatgta acaacttaat ctcagggtct tgagataatc
     2461 cccatgattt tccacatata aatccaactc cttgaagtga ttttataaga gcataaattg
     2521 tatatggtga atcagactca aatgctattt tgggtttgaa attagctgat tcgcacagga
     2581 tatcaattac ctctctataa ttctcaccct tcgttataac tataaagttt tcatctgaaa
     2641 cttcgcttag atatacttca tcctttaatg ataaaggatg gtttattgaa actcctaata
     2701 gtatttcttc gcatgttaaa gttatgctat tttctgagtt tagctttgta aatgaagaag
     2761 atatgtacaa atcaaaatca ctttttttat aagatgatgg caaagtatgt gacacattaa
     2821 aagtaatatt gggatatagt tttctgaaat tactcaaaag tgatggtaac acattagccg
     2881 ctgcaggaga taatatcttt acttctcctg aattttctaa agaagtgtct tgtaactcct
     2941 tttttgaact ttctactaaa tctaaaatgc tatctactct ttttagaaac aatcttccat
     3001 actcatttaa aacaatatat cttccttttc tgtcaaataa ctgaactcca agttcttttt
     3061 ctaaggaaga tatagttttg cttaaagctg gctgtgctat atttaacttg tgtgatgctt
     3121 ttgttatgtg ctccattcta gcaacagttt gaaaatactt aagatgcaat aaatccatag
     3181 tataaaatac acctctttta atatgacttg agttatatca attttataat caactccaca
     3241 ttatacatta taatataaag tttatataat aaactacaat atattaaaaa gttatttaaa
     3301 ttgttattta tattttgtac ataatctaat taaaaaaagt aactttaaat gattaactaa
     3361 ggaaatgttg ttacaaactt tagttatata aaaaattcat ttgatgagca aagtactaat
     3421 atcaagtaag aataaaagct tattagataa gaaaaataat attttataat aatgatgtta
     3481 cttaaattat tatgttatac tattttaaat aggaaaaatt tagatactat aattttaggg
     3541 ggagtcaaga tgaaattaac taaatcaata tcatcaatgc tgtgcttatc tctagtttta
     3601 agcttatcac cagtagcata tgcaaatgaa ataagtagaa caacagttga gaatacttta
     3661 gtaggtcagg atagatatca aactgctgtt gaagtaagta aaaaaggatg gagtagtgca
     3721 aatgaggctg tagttgtaaa tagtgaatct attgtagatg cattagctgt aacaccattt
     3781 gctaaattaa aaaatgctcc tatattattg acagaaaaaa ataatctaaa ttcacaaact
     3841 gagaaagaat taaaaagatt aggagttaaa aaagtttata taattggtct atctagttct
     3901 gtatcaaatg atgtgcaaaa acagttagaa tctaataatt taactactga tagagttggt
     3961 ggtagtgata gataccaaac agctttgagt atagctaaaa aaatagaagg attaaaagat
     4021 atatcagaaa tagctgtagt aaatgggtac acaggtcttg cagatgcagt tagtataggt
     4081 tctgtagctg ctactaatgg aatggttata cttccagtat ctgatgcatc tggtatatct
     4141 agctttaaag attttataga atctaagaat ataaataaat catacataat aggctctact
     4201 aatgctgtat cagataaaat aaaacaaagt ttacctaatt cagaaagaat aggtggagca
     4261 gatagaaatg aaactaatgg taaagtaata gaaaagtttt atacatctaa taaattaaac
     4321 aatgtatttg tggctaagga tggaatgaaa aatcaaaatc aacttataga cgctctagca
     4381 gtaggagtac tagcatcaaa agaaaattct cctgtactaa tcgtaggaga acaattaagt
     4441 gcaacacaaa agagtgtatt ttcgaataaa aatgctacct ctataactca agttggtagt
     4501 ggaggaaatg aaaatgcatt taaagaatta aaagatttac aggcaaatag taacaataag
     4561 agagtaatgt atgtaactaa tacagataag gtaaatataa gaaatgatgc tacaatagaa
     4621 gcttctgtta taggatatct aaataatggt gatgaagtag aagttttaga tgtgttaaaa
     4681 actggatggg taaaaataaa atacaatgaa ggtattggat atgttagtgg gtcttatttg
     4741 acaaataata agcctgataa tagtaatgaa aatataaaaa taaagtatgt aaaagaaaaa
     4801 gatggtctaa atgttaggaa aggaccaagt acagaagatg aaaaaatagg tcacctttca
     4861 tatggaagca aagtagaaac aatagaaatg tttgctactg gatgggtaaa aataaagtac
     4921 aatggtggat atggttatgt aagtaatgat tacttatctg atacaccagt tatttaatta
     4981 taagtaacaa taaaaaaact gctttaattt gtaaagcagt ttttttataa aaatttaagt
     5041 ctaataacta tttctatttt gataccttaa tcctgggatt tattattatg gatattatca
     5101 acatacaaac actccaaatc cagtgtctaa ttccaaactt agatattaat aaattcagtc
     5161 ctgctatggt aaataagcag tatttaatta caattaaaag tgtatttata gaaagccccc
     5221 tcatagaaag tatcctaaat ccaataatag acattctaag tcctataaat gccaataata
     5281 tacctgcaat atcctgaatt aagtaatagt attcaaattt catattcaat caactcttct
     5341 ctgagcagta tcaaatattt ctaactcttc aatttcatca tctgtgtatg taagcatagt
     5401 aaaaccagtt acaccataaa taaatgaaat tataggtgta atccaactta agaaacaata
     5461 tggaataaat tctagtggac ttacaccaag agtctgagca cagaaaacag catttgaatt
     5521 ccatggtatt aatggaccac caagtgtagc tgtatcttct ataattctag atagattttg
     5581 tggttttaat ttaaattctt tgtatattgg agacattaga gttccgacaa atacagaagt
     5641 gaacatcatt gatgaactaa atgcatttgc aaagtagctt actataaaag tagacccaac
     5701 caatttagtt cttgattttc ctattttagt tataagaggt tgaagcagta cttctaagaa
     5761 tccagttttt tggagaatac cagcaataac gaaaactaca aatactagta aaacggtttc
     5821 agccatactc attattccac cacgatttaa tagtttgtcg gcatagacaa atccagtatc
     5881 tatagtaaat cctgatagca tgtaatttaa aacatcacct attttttctc cttcttgaaa
     5941 tactgctata ataagtccca tgatagctga acttagtata gatataatgg gtggcttttg
     6001 tagcaataaa agtaataata aaaatattat tggaatcatt gctactaaac caatatgaaa
     6061 gtttgaattt aatacatcct tgatttgatt tatttgaaca tagtctatag tattatttga
     6121 atacttaaat ccaattactg tatacagaac tatacatatt acgtatgcag gtcctgtggt
     6181 ataaaacata tgcttcatat gtgtaattac atctgtctta caaactgctg cagctaagtt
     6241 tgtagaatct gatagaggtg acatcttatc tccaaagaaa gcaccagaaa tgactgcacc
     6301 agctgttaaa ccaagaggaa tacccatact agtaccaata ctcatcattg ctataccaga
     6361 agttccaaga gtcccccaag atgtaccagt agcaacagaa gttaatgaac atacgattaa
     6421 tgttgtaagc agaaaatatt taggggttat tattgttagt ccactatata ctactgttgg
     6481 tattgtacca gcagctatcc aagctgctat tagtacacca acagaaagta taattaagtt
     6541 tgattgaaag gcatctttgc caacttcaaa tccaaaagct tctatttcat cattagtata
     6601 accaagtttc atggctgcag gaactactat aagccatgaa agtaaaaaca ttgtagttat
     6661 agaagcatta aatacaacta taccaactga tgttagagct attattgcaa gcattactaa
     6721 caaggaatat ctaaatgaag gtatttttct ttctgtattc attttcatag tgcaaattct
     6781 ccttaaatat ttttagttaa ttgcattttt tattcttata agagcttctt caagcatact
     6841 tcttggacaa gctaagttta ttctttgata cccacttcct ccaatgccaa agctgtttcc
     6901 ttgatttaga gcaactttac ctttttgtac aagtatactt tctagttcct catcacttaa
     6961 tccaagtgca ctaaagtcta cccataataa ataagtacct tctggttttc taactttaag
     7021 ttttggcata ttttcattta tatattttat agcaaaatct atattagatt ctaaatattc
     7081 taaaaatgat tctagccaag attcaccatt attatatgaa gcttcagttg caactaagct
     7141 aaagcaatta tttctcttta tatcaattct ggtaaaggca tcatctaaca atttatagta
     7201 tttttcatca ggtagtatta catatgatga ttgaagacca gctatattaa aagttttagt
     7261 tggagccata caagtaattg tatttttttc aaattcttta gaaatagatg ccataggaat
     7321 atgcttatgt ttttttaata taatgtcact atgtatttca tcagatatga tttttacatt
     7381 atgcttaaga caaatatctc ctagtttttt taattcatct tttgtccata ctctaccaac
     7441 tggattatga ggattacaaa gtatgaataa ttttacatct ttaatcttgt tttcaatgtc
     7501 ttcataatcc attatgtagt ttccattttc taatttttgt aatgggctta taattagttc
     7561 tctattgtta ttttttacaa cactattaaa agggctgtaa actggttctt gtatcattat
     7621 tttatcattt gcttttgtga gttcatttat taaaagactt atagctggga taacacctgg
     7681 actataaatt aaccactcac tttttatttt ccagttatgt ctcctatcta gccagtttac
     7741 tatagattca ttgtaagaat ctggtcttgt tgtgtagcca taaatctctt gttctagtct
     7801 attttttaaa gaatcaatta tacaaggtgc agccttaaaa tccatgtcag ccacccacat
     7861 aggaagtaaa tcattagttc catacttttt ttccatttct gaccatttag atgaaaaatt
     7921 attgcttcta tcaacaatct cattaaaatt ataattcatt gtaaatacct ccagtaattt
     7981 taattttatg ttaataatat taagcaaaca acgtgccaat attgataata aaaaatattt
     8041 attcacaagt taatatttaa tattaagtaa tttcagagca tagataagat aaaaatatta
     8101 attatactta aaactttgtg gaaaaggtta tttctaataa atttttaaat atatgaaaat
     8161 gtaattaaat aggttaaata ggtttaactg tttaacttgt tagacctatt tatatttttt
     8221 agaaactcta ctccctttga tgttataatt gaacctccac gaccaacaag tatgtctaca
     8281 agcccaaagt cttgtagttc taaaagcata tttctaatct gttgttctgt caaaaatcta
     8341 ttttcctcta aagctatttt acataagctt tttctaccta ttctttcttt taaatcataa
     8401 gcctttttta aattatccaa tataaatatg tattcatcta gtgacctttt aaaattatag
     8461 tcgtatttca tgatgttttt gtctttgcca atgttgaatt gatacttttt tatattatta
     8521 cttactttat tacaaacggt tctactataa tttgaatcta tagtatctaa tatatattca
     8581 ggtaaatcac atatttctat aatatctttt tccaagtaac agaaatattc tcctaagttt
     8641 cttaattctc ttacatttcc ttcccatcta tacattttaa agatttcttt tacttcctca
     8701 cttagaataa aattacatgg aatatcatat tttaatgaac caaaaatttc aaatatatca
     8761 gcaggtcttt ccctaagtgg atgtatttta aggggaagaa cattcaacct aaaatataaa
     8821 tctcttctaa acatattttt agatacaagt tcttttaaat ttctattagt agctgcaatt
     8881 attcttacat caatgtcaat gacattatta gaaccaatac gtcgtacttg tttttcttgt
     8941 atgactctaa gtaatttaga ttgagaattt aaatccattt ctccaatttc gtctaaaaat
     9001 atagttccat tgttggctaa ttcaaataaa ccaattttcc cacctttttt agcaccagta
     9061 aaagcccctt catcatagcc aaataattca ctttgcagta gattttcttg aaaagtacta
     9121 caatttactg cgacaaaagg accatctttt ctcctagaag cactatgaat ggattgagca
     9181 aataattctt taccagtacc actttcacca attataagga tagatgaatt tgattgagcc
     9241 attttattag caattttttt tgtattaata gtttgtatac tagaacctaa tatatcatca
     9301 aatgtgtatt tactgatatt accagagttt actagttgtg ctcttaattt agcttgcttt
     9361 ttttctaatt gagaaaactt tgtcatcttt agtataaatc catcaaatac atttaattgt
     9421 atatgtttaa tctctaaatt tatatcaata tgatttattt taatcaattt ctcaaaaaat
     9481 ggagttttat tggtcaatat gctttgaaaa ttgatgtctt ttatgcaatc accaacaaat
     9541 gagtcaatca tttcatttgc atttatagac aaaatttttc tagccatatg attatagaat
     9601 tgaattattc catcgttatt tgtgcatata attccatcat caattataga tagtaacaaa
     9661 tcaaattgtc tttcaaactt atttgcatct aacaataact cacctgtact ataacttgta
     9721 ggaattattt tttctacgta tttttttatt aggtcatctt ttattagatg ttttaatttg
     9781 gtttttgtag caatttcaac aatacaactt aaatctatta ttctgtattt taaatctata
     9841 acttcacaat ttttaaactt aggcaagatt ttttcgccag gagttaggat tacagcatct
     9901 gtgggagtaa actcaatctc tggatagcag ggcaggatat ttatattgtc tattcctaat
     9961 ctatgtatta aagaaattgt ttctattgcc atatctttac ttaagttata tacataaata
    10021 attttatctt tttctaattt ggatatcata tcaatactgt tcttttcaaa tgtcaactta
    10081 ggaacaatga tttgagcatc atagtgagat aattttacta tttcatcata ttttaaataa
    10141 gcagatagga gtataagttt ttctttaaaa aactttaaat ttccttcttc aaatgagtat
    10201 aaatttatct ttagattatc acctaagaat tgtcttattt gattttcata aatttctcca
    10261 gcgtcttttt ttaaacttac aatagctaat tccatttgtt tcattttaac ctctcataca
    10321 aatttgactt attttagatt acataataat taaattagat taaataatat tatttaatta
    10381 ttattataaa tttattatag actacacatt gaagtaaaag aagtagctaa aattattcct
    10441 tttatttttg ttttttgtat ctatttggta taatagtatt agagcgtata ataatttagg
    10501 caaaattttt taaggaagac tagaggagaa tataaaatga aaaaatgtct ttactgtaat
    10561 agggatataa agtcaccaaa taataaatta gctataaaat atgatgacaa tatgaatata
    10621 tatccatgtc gtgcagaatg taaggaaaaa attgaagaat atatagcaaa aaaacctaca
    10681 tataagttta taaatatatt agaggttctt ttaggtgtgg gcggaatatt ctgctttttg
    10741 agtaaatgga ctattgcagc ccaagttgta cttggaattg atgcactggt tatattttta
    10801 ttcccattgg caggatttaa gatgaatgga aagttaagta tggaacaaac taagaatcaa
    10861 agtaggtcta ttgcaatatc aattttggca tttatagtaa taataactgt attatatttt
    10921 aaacaaattg gtaagtagag cataattgta taagacatca ttagagtatg aggtgagttt
    10981 atggaagaaa gaaaattagc accaaacaat gagagggaag ctctcataaa aagattaaaa
    11041 agaatagaag gtcaagttaa gggtatacaa aagatggtgg aacaagaacg ttattgtgtt
    11101 gatatattgg ttcaaatatc tgctataagg tctgctataa atagagtagg cacaattatc
    11161 ttagagaatc atataaaagg atgtgtagca gaaagcatta aacatgatga tgctgataaa
    11221 actgaagaga tgataaaaga gcttatggat actataaata aatttacaaa ataaggaggc
    11281 ggttagttat gaatcaacaa ggatttatta tggaaattgt tgatgataga actgcaaagt
    11341 taaaaatgca aagacattca gcttgtgctg cttgtggaaa atgtgctact acaaattcag
    11401 cagagtcaaa agaaatatta gtagaagtag acaacacaat tggcgcaaaa gttggtgacc
    11461 atgtagaagt gagtatggat aatatgaatg tattaaaagc agctgtaatg gcttatataa
    11521 tacctcttat agcacttctt gtaggaacaa tagggactta ttatatattg ggggcaattg
    11581 gaatgacaac tggagttgaa gccataagtg gattagttgg aatttgtctt acaataatct
    11641 gttatttata cttaaagaag aatgacaaaa aatttagaga cagtagagaa tttataccag
    11701 taataacaag aatattaatt gacctttaag ataattataa ttaaaaaata gcaatgtgct
    11761 tcttaaataa atactcaata cgtatttgta gagtttaagt acattgctat ttttatattt
    11821 ttattttgat tttctgtaag gaataaagtt ttttctgatt ttcaaaagat tcaatgttct
    11881 tacaaaagaa gttacaacta atacaccaag tgctattgca gcacttagaa atagcgcatc
    11941 tgtacctttt gacatagcca aagaataatt tttctgaatc aaagacaaca ttgtattata
    12001 tagcccaatt cctggtatta gaggaagaat acctggaatt acgaacacaa tagctggttg
    12061 ctttagtttt cttgctaaag tctcactgca cgctgagacc aaagcagcag cgataaaacc
    12121 tgcaaaaact gcatttgtag taaaattaaa taaataaaca tatacaatcc aaccaattcc
    12181 gccagttact ccagctggaa gaagtagata tagtggagca ttaaagaaaa cagcaaatcc
    12241 agcagttgca acacctgaaa ataaaaaatg caaaatcata gacatatcag tcattagaaa
    12301 ggacctccta ttctatacca tgtatcaagg acaaaaccaa ctccagaagc aattgcaata
    12361 ataatcatag cagcatcaaa agcacgagaa attcctgata ttaaattccc tgatattaaa
    12421 tctctaatag cttttataaa agcaactcct gggagtagtg gcattatagc gccgactata
    12481 agcattttag ggtcatctaa aatgcctatt tcaactagta gaactcctga aagtgcaatt
    12541 aaaaaagaag atactaaaca ggaaaatgca gatattgcac ttatcttcat cgttttatca
    12601 taagtaattg cagcaaagat tgctattata aacgtaagta taaaatttaa tacataattc
    12661 cctccaagca aacaagcaaa acaagcagat gcaattcctg tacaagagta aaataaccat
    12721 aaaggataag gttttacatc ttggatttca taaagacgtg caatggcatt atcaatatca
    12781 tactctttgt cactaacaaa ttctcgagaa aaactgttta ataaagaaat cttatttaag
    12841 tttatacctc ttgattgaat cgttttcata aacgaaaagc catcaaattt ctcatctgaa
    12901 ataattatta ctgtaggagt tgtaaaaata tttacatgat aaaaccctct tgacctgcat
    12961 attctaagga cactgtcttc aaccctagat gtttcagctc cattttttat catcagttcc
    13021 cctacaaata gagctagttt tagaatattt ttcttatatt caagttcctc attttgattc
    13081 ataaatacac ctccaaatta aaaaacgtaa tattgaaatt atatcaaatt tttaagtaat
    13141 attccttaat atctatacaa atatattatc ttttttttgt ttcaaaatag tcacatttaa
    13201 attatatcca attaaaagaa ttagacaata ttatttatta tattttaact taaaaataaa
    13261 gaaagtaact atgttaaata tggaaaaatg gcaaaacact tgactattaa ttggtaaagg
    13321 actataataa taaattatca tattgataaa aacgaaaatt atgaaatgtc tgtaaaaata
    13381 aaatttaaat aataactagg aggagtaata tgtttgagaa cttgaaaaaa gcggaccctg
    13441 agatttatga aagtatgaaa agagagttaa aaagacaaca aagaaatatt gaattaatag
    13501 catctgaaaa catagtatca gttccagtaa tggaaactat gggaagtcat cttacaaata
    13561 agtatgcaga aggttactca ggcaaaagat attatggtgg atgtgaatac atagatgaag
    13621 ttgaaactct tgcaatagat agaatcaaaa aaatatttgg agcagaacat gctaatgttc
    13681 aacctcattc tggagcaaat gcaaatattg gtgtatattt tgctatgtta aaaccaggtg
    13741 atgttgttat gggaatgaat ttatctcaag gtggacattt aactcatggt gctccagtta
    13801 atatttctgg tcaatattat aaattttatg aatatggtat agataaagac agtggactta
    13861 tagattttga tgaagttaga aagatagcac atgaagtaaa acctaaaatg atagttgcag
    13921 gagcaagtgc atatcctaga gaaatagatt ttaaaaagtt tagagagata gctgatgagg
    13981 ttggagcatt gcttatggta gatatggctc atatagcagg tcttgtggct gctggtcttc
    14041 accaaaatcc atgtgaagtg gctgattttg ttacaacaac aactcacaaa actttaagag
    14101 gacctcgtgg gggtgttatt ctttgtaaag aaaaatatgc taaagacata gacaaagcaa
    14161 tattccctgg tattcaaggt ggacctcttg aacatataat tgcttctaag gcagtttgct
    14221 ttaaagaagc tttaagtgat gagtttaaag aatatcaggt acaagttgct aaaaatgcaa
    14281 aagccttagc tgaagaactt attaaaagag attttaaatt gatttctgga ggtacagata
    14341 atcatttaat actattagat ttgactaata aaaatataac tggtaaagca gcagaaaaga
    14401 gattggatga tgcttatata acagctaata aaaatacaat tccatttgac ccaaatggtg
    14461 ctcttgttac aagtggaata agacttggta ctccagctgt aactactaga ggtatgaaag
    14521 aagaagatat ggcaataatc gctgaggcta tagacctatg tttaacttat gatgaagaat
    14581 ctaaggctag aacattggtt gttggtttaa ctgaaaaata tcctttatat gaagaatata
    14641 atattatgga ttaattataa agaacaacta aataaaatac atttaaataa ttacaatgat
    14701 tgtgtatgta tttgaatata tttagatata aaaaaggtgt ctcaaaatga actttttagt
    14761 ttatgaggca cttttttgta tggaatcgaa catatttgta ttatttaaat aataaaaaat
    14821 atggttatct ctatttttag atggtctctt tgtatttttt aattaaaact ttaagatttc
    14881 ttaagaatta tactaggttt ttctaaagat ttaaagtcta tacttataag tatagtaatc
    14941 atatgaaaag aaaagcttta acgaggaggg aatcttatga aggtattaat tcttacagga
    15001 aaatttggta tgggtcatta ctcagcatca aattctttaa gtgaagatat aaaagcaaaa
    15061 tttgataatt cagaaataat aattaaagat atatttgagt acattatgcc taactattca
    15121 gataaaatgt acaagacttt ttctattctt gtaaatcggg gaagtagtct ttataatttg
    15181 ttttataaat gtgctgaaaa tggaaagaaa gatataaaat ttactttttc agattatttt
    15241 ttaaataaat tagatacatt acttcatgaa gtacaaccaa cagtagttat ttcaactttt
    15301 ccattttgtt ctcaattagt atcaagatat aaggaaaaat ataactcaaa tcttccttta
    15361 ataacatgta taactgatat aagtagtcac tcagaatgga ttagtaaaaa tacagactgt
    15421 tatttagtag ctagtaaatc tactaaggaa gaacttgttt ttaaaggaat agatgaatct
    15481 aagattaaag ttaatggtat tccagtaaaa aaagaattta aaagaataga acatgtaaat
    15541 cattcaacta aaaaaaatat tcttataatg ggtggaggat taggattact gcctaaatca
    15601 gaacaatttt acaaagaatt aaatagttta gaaggagtaa aaactactgt aataactggt
    15661 aataataaaa aaatgtacta taaactatat ggtagatatg aaaatataga ggtagtaggt
    15721 tacaccaatg aagtatataa atatatgaaa gattctgacc ttattatttc taagccaggt
    15781 ggaataacac tatttgaaac aatttattca gaacttccaa tattggcatt taatccattt
    15841 ttacaacaag agatagataa tgctagtttt atattaaata atgaaattgg gagaatactt
    15901 ggtaaaaata aaaaatacta tgtagatgag ataaaagatt taatttatga tgatgctact
    15961 cttaaagaaa tgagcagtaa catgaaagaa ttaaaaaaac aatttgataa taatacctta
    16021 gagaatattt tattttcact tgatgaacaa ggagcgtgta gagaatgtat gtagtaggtt
    16081 taattatcgt tgttgcattg atttttttag tccattcaat tatacctaca tactataata
    16141 aactattaaa taaagaggta ttaaaaaata tggctggaga aaatgaaatt gcacttacat
    16201 ttgatgatgg tccagataag agatatacag aaaaattact agatgtgtta aaagaaaatg
    16261 atatacaagc tatgtttttt gtagtagcaa agaatgctga aaaagagcct gaaattataa
    16321 aaagaatgtt gagagaaaat catatagttg gtttacattc attggaacac agaaatgcat
    16381 ggctttatag ttatagttat gttaaaaaag attttattga aagtacaaat ataatgaaaa
    16441 atttaggtgt agatgtaaac tactatcgtc caccatgggg tcatactaat atattttcaa
    16501 attcatttgt taaaaaatat aatctaaaaa tgactttgtg ggatgtaatg gcagaagatt
    16561 gggagaaaga tagtacagta gacattatta taaacaagtt aatgagtaga acaaaagaaa
    16621 attctataat ttgtttacat gatgcaggtg aaaattcagg tggtgcagta ggtgcacctg
    16681 agcgtactat tgaagcttta aagatagcta ttccaaaact taaagccagt ggtttaaagt
    16741 tcgtaacacc agaaaggatg tagttatgat aagtaaaact gaaaagaaaa ataaatgcct
    16801 aggtagtgta gcttttttag ttttactaat gggcataaca ggatattttg tttttagagg
    16861 gcagtcagtt gaatcattga taaaatcatt aaaaggtgca agccctatgt ttatactaat
    16921 tggatttgca atgatgttta tatatgttgc ctgtgaaggt ataaatatat acttaggtat
    16981 gaaagcttta aaccaaaaga ctactctttt aaaatgtatg ggatatgctt ttattggatt
    17041 ttattttagt tctattacgc cttcagcatc aggtggtcaa ccagcacaag tttattatat
    17101 gaaaaaagac gatataaata tatcatattc ttcacttata ctattagtca ttgttgttat
    17161 tcaccaagta gttatactgg catatagtgg aataatgttc attatggaac gtgagtttat
    17221 attaaataat gtaagtggta tgaacatttt actaatatat ggtgtaataa caaatgttgc
    17281 tcttgtcatt ggagtaattg caattatatt ttcaaaaaaa cttgtaaaca attttataat
    17341 atcaataaca aatttattag gtaagttaag aataataaaa gatgttgaaa gttcaagaaa
    17401 agttataata tctcaaattg aagagtatgt taaaggtgct caatatataa agcagaatcc
    17461 aaaacttgta gtccaaattt tagtcataac tattgtacaa ataacatcta tgtttttagt
    17521 tcctttcttt gtatacaaag cttttcatct atcaacatat actgtatttg agatattagc
    17581 gatacaatcc ttgcttaata ttgctgtttc ttcattacca ttgccagggg ctgttggagc
    17641 ttctgaaaat agttttatga cattatttaa gatatttttc cctggtcact tattagttcc
    17701 ggcaatgtta cttagtaggg gaataagttt ttatgcattt gtagctataa gtggattgat
    17761 atgtatagta gtacatgtaa aatcttctga aaaagtaaaa gcagtgaaaa aagttgctta
    17821 tgtaagataa tattatatag attattttta ttaatatgtc aatttgtgtg tatattttaa
    17881 ttaagcttta aacatgttta caggagtaga aactattaaa acgtgtgaca ttgctaagct
    17941 tttgctaata aagtataata ttttatatag tttgaaatat ttaagaaata tagcaaaaaa
    18001 aggagcttgg tgatgaataa agagattcca aaaaaaattt tagtagctga tgataatcct
    18061 gagattaggg aaatcgtaga aatactttta actggtgaag gttatgaagt tattatggca
    18121 acgaatggag aagaagctgt aaacttagta gatagtacga ttgatttgat tattttggat
    18181 gttgtaatgc ctgtaaaaac aggatttgtt gcatgtaatg aaattagaga aaaaacgaca
    18241 gcaccaatat tatttttaac tgcaaaaact caagactctg ataaagttat tggattttca
    18301 gcaggaggag atgactattt atcaaagcct ttttcatatt ctgaactaat ttcaagggtt
    18361 aagtcgctac ttagacgtta ttatatatac cagggaaagg aaaaggaaag agagccaaat
    18421 atattaaaaa caaaagagtt atgtgtcaat ttagatactc aagatgttac actaaatgga
    18481 gaacctgtgt ttttgacaac tatagaatat agtatattaa ttcttatgtt gaaaaataga
    18541 aaaaaagttt tttcgtctga aaatttatat gaaactattt gggatgagca gtatttttat
    18601 acagcaaata atacaataat ggttcatatt agaaatttaa gaaaaaagtt agaaaaagac
    18661 caaaaaaatc cacaatatat aaaaaccgca tggggaaagg ggtattatat tgattaacaa
    18721 aattaagcca tctaagttgg gagttatact attgatatat gtagtaatct cagctgcagt
    18781 ggctttcatt ttaaatatac cattttactc tgctgttgaa tctatagtaa aagatgtatc
    18841 agaagggaaa gtatatttta aaagagaagt aaatcaactt atggataatt ttcaaactta
    18901 tgtgaataac aatggtataa gagcagatga tggagatgat atagcaaaat ggaatagtga
    18961 taattggttt gtttctatga agatatataa aggaaataag gttttttatg atacattata
    19021 ctatcctaat gaagtacctg ataagagtga ggaaaatgag ttttatagca gtcccatggc
    19081 tagaaactat gagatacaat tttctgatat taaagctaat atagttgtag tagctttatt
    19141 tagatttaga tttcaaaatt ttatagaagc aatttctatt acatttatgg cacttacatt
    19201 tattataaca tttttactgt tgtttagtag aaagataaag tacctaagga aaatagaaat
    19261 gggtataaag atagtagaga gtggcagttt aaagtataga atcccagtaa aaggtaatga
    19321 tgaattatca tcacttgcat ctagtattaa tgagatgagt catacattgg agaatcaact
    19381 tataaaagaa agggagatga agaataaaaa taaagatata gtaacttcga tatcacatga
    19441 tgttagaact ccattaacgt cagtaatttg ttatttagat ttgattaaag accataaata
    19501 caatagtcct gagcaattgg aacaatacat aaaaaatgta aggtctaaag catatcaaat
    19561 aaaagattta acagatgacc tttttacgca ttttgttaat tctggagagg aagtttcaaa
    19621 agggtatgaa aaaataaatg ctaatgaatt tatttttcaa ttacttttag atagttcata
    19681 tacattggag gagaaaggat ttcatataac aatagaaaat gaactttata accaatatca
    19741 tatttatgta gatgttacac aatttagaag gatatttgat aatttatgtt ctaatataat
    19801 aaaatatgct aggaaagata agcctatagt atttttaatt aaagtagagg attctaaatt
    19861 actaattata caaaaaaata gagtaagtaa tttttctaag aatgtagaaa gttttggaat
    19921 tggtataaag aattgtgaga aaatagttgc atatcataat ggaagcataa atgtaaattc
    19981 agatgataaa gaatttgcta ttgaaatatc tattccagca aaagaatcat aaaaatttaa
    20041 attatatatt gtgtaattgc tattatatat tgtgtaattg cttatgtaaa attctatgaa
    20101 gcaggaagca tgtgacttta gttatgggat gttcaatatt gtgtacctct aggcaagtaa
    20161 caagtttact tacaagacca tataatatta ttatcttaaa aaaaggggac ataataatat
    20221 ggttttgaca ggagcacatt atatttacat tctatttatg gtaattatat taattactat
    20281 gattatgaaa aaggatacaa tagtaccatg tatattaggt gtttttttta tgggattgtt
    20341 ctttgaaaaa aacatctttg gtgctataac agcagtgttt aactctttta ttatatcttt
    20401 aaatgagttg ggtccaatta tcttgattat agctattatg gttgcattat ctaaagccct
    20461 agaagccaat aatgctatac agtatatggt gagaccattt tcgagagtta taaaaaattc
    20521 taatacagca ttttttgtaa ctggatttgt tatgttagtt ctttcttggt ttttctggcc
    20581 aactccagct gttgccttag ttggagctgt atttttacct gttgctatga gagctgggct
    20641 accagcaatt ggtgttgcag tagcattaaa tttatttggt catggtcttg ccttatctac
    20701 agactttgtt attcaaggag ctccaagtat aacagctggt gcagcaggtg ttgcagtatc
    20761 atatgttata aatgatggta tgattttgtt ttgggtgatg ggaatcgtaa ccatatctat
    20821 ggcattttat acattaaaaa gagatattaa caaaggaatg tttagagaag aactaaaagg
    20881 ttttgaaagt gaagaagtag aagcgttcaa tggaaaatct aaaatagcaa ctattttagt
    20941 agctttggga tttcttgctg atataattgc tatgtatgta tttgacctaa aaggaggaga
    21001 tgcatcagct cttttgggtg gaacagcagt atttttaata ataattataa atgttataaa
    21061 ttttggcaaa gatagtcttg aaaatgtatg tgagaatata attgatggtt ttgtgtttgg
    21121 gataaaaata tttggagcaa ttataccaat agcagcattt ttctatatgg gtgaagttgc
    21181 accattgaca ggtgtatttg gaaaagtatt agcaccaggt tcacaaggtt tattatcaga
    21241 tataggcata gctctttctc aaacaatacc acttaataaa tttgccgtat caggtataga
    21301 aactgtagtg ggagcaataa ctgggcttga tggttctgga ttttctggga tttcattagt
    21361 tggttcactt gcctctgtat ttggaactgc tataaatgca agtgttggtg cattagctgc
    21421 attaggacaa atcagtggta tatgggtagg tggaggctgt ctagttcctt ggggtcttat
    21481 ttcagcagcg gcaatttgtg gggttagtcc aatagaattg gcaaagcgaa actttgtccc
    21541 agttataacc ggattggttg taacaaccat agtagcaatg tttattattt aataaatcga
    21601 ataaatcttt aattgtataa attaaaaata gatgtgtagc tataatttat tataattgtt
    21661 ttactaattc taagccatac atctattttt attgatattt ttattagtat tctttaataa
    21721 agtaatattt ttcattatat tatacataac atctaaatat aacatctaaa atactgttat
    21781 ctatatttaa tatcttgcca aattttttga tgttttattg ccctgtccca ccaacatata
    21841 taagctatca caagttttta cctatactat caccaaacaa aaattctaat tttaatttat
    21901 tattttataa cctatattat attaactata ttagtttgta gactttagat gtaattataa
    21961 atattaatac tccaagagtt caaattcata tcctagtttt cttgcatttg atataaaatc
    22021 atttaaaatt tctgtactag ttttggaaac tgcatgtagt aacaagatag aaccatcatg
    22081 tagattatcc attatttttt gcgttgcttc ttcatgtgat ggttgtttat cagtatccca
    22141 gtctctgtat gcaaaactcc aaaatacagt cttgtatcct aaatttttag tcattgccaa
    22201 gcttttttct gagtattttc ccataggagg tctaaaaaat ttaaccatat ctttcccagt
    22261 tacatcttta tataactttt caacatcata taattcgtca ttaaagtttt ttagattgct
    22321 tgtttttgtt ggcatagatg gatgggtttt actatgattt ccaactatat gtccctcaga
    22381 aaccattctt tttactaaat ctttattttc ttttatatat ggagctgtaa caaaaaaaac
    22441 tgctttaacc tggttttgtt ttagagtgtc gagtattttt gtagtgtatc cattttcata
    22501 accttcatca aatgtaagat atagtgtttt ttgaccatct tttgtaggtc cattgtatag
    22561 ggcatcatag tcagaaaatt taaattcaat gtccgtattt acttctggtg ttttatgctt
    22621 attgtttggt atataaaacc aatcaagagt agtgttatct aaagaagata catctaattt
    22681 attattatca ttattagtag tggcttttga tggtttatct gttccgtctg aaactattac
    22741 attggaagta tctttaccac tatctatttt ttccttatct tcttgtaact gtgtttcctt
    22801 attttgattt tcgttttgat tgttttgtga atttgaacat ccaacaataa aaaaaataga
    22861 aatagaaagt agtgttaaat aatgcaatac ttttctaaac attttgaagc tcctttctaa
    22921 attaataata attgcatata ataataatta tatgattaat tacaattgca tatagtatta
    22981 ttataattgg aaattactat taataatagt tatatcttta aaaataaagt cacaatagta
    23041 tgacaaaggg tgaataatca ataaatttaa aaaaaaatga aaaaaatagt tgaccatatt
    23101 atgagaagat gttattataa taaatgtgct caacaagaag taacaaaata acaagcacaa
    23161 agaagttaat tgaat
//
LOCUS       DAMKMI010000050        22798 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMKMI010000050 DAMKMI010000000
VERSION     DAMKMI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.051"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..665
                     /locus_tag="PC429_001827"
     CDS             <1..665
                     /locus_tag="PC429_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3847334.1"
                     /translation="ISKEPSGRYYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSC
                     GEFIENPKYLKKSLNKLAKLQRELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQK
                     LSTKLIKENDIICIEDLQVKNMIRNSKLSRLISDVSWSEFIRQLEYKANWYGRQIVKV
                     GKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDRDINASINILKEGLRLITIQ
                     NK"
     gene            816..2033
                     /locus_tag="PC429_001828"
     CDS             816..2033
                     /locus_tag="PC429_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF3847335.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLITFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILIYNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISRFIGSLFGGIL"
     gene            2036..2776
                     /locus_tag="PC429_001829"
     CDS             2036..2776
                     /locus_tag="PC429_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3847336.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            3099..4409
                     /locus_tag="PC429_001830"
     CDS             3099..4409
                     /locus_tag="PC429_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF3847337.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            4507..5424
                     /locus_tag="PC429_001831"
     CDS             4507..5424
                     /locus_tag="PC429_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF3847338.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            5440..6249
                     /locus_tag="PC429_001832"
     CDS             5440..6249
                     /locus_tag="PC429_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF3847339.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDTMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            6307..8055
                     /locus_tag="PC429_001833"
     CDS             6307..8055
                     /locus_tag="PC429_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF3847340.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            8317..9339
                     /locus_tag="PC429_001834"
     CDS             8317..9339
                     /locus_tag="PC429_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF3847341.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            9344..11650
                     /locus_tag="PC429_001835"
     CDS             9344..11650
                     /locus_tag="PC429_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3847342.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            11622..12737
                     /locus_tag="PC429_001836"
     CDS             11622..12737
                     /locus_tag="PC429_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847343.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            12755..14173
                     /locus_tag="PC429_001837"
     CDS             12755..14173
                     /locus_tag="PC429_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF3847344.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            14188..15396
                     /locus_tag="PC429_001838"
     CDS             14188..15396
                     /locus_tag="PC429_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847345.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(15470..16858)
                     /locus_tag="PC429_001839"
     CDS             complement(15470..16858)
                     /locus_tag="PC429_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF3847346.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            17199..18533
                     /locus_tag="PC429_001840"
     CDS             17199..18533
                     /locus_tag="PC429_001840"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF3847347.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(18675..19592)
                     /locus_tag="PC429_001841"
     CDS             complement(18675..19592)
                     /locus_tag="PC429_001841"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF3847348.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            19800..20684
                     /locus_tag="PC429_001842"
     CDS             19800..20684
                     /locus_tag="PC429_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF3847349.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            20897..22738
                     /locus_tag="PC429_001843"
     CDS             20897..22738
                     /locus_tag="PC429_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF3847350.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
BASE COUNT         8803 a         2456 c         4002 g         7537 t
ORIGIN      
        1 ctatatctaa agaaccaagt ggtagatatt atgtatcatt atgctgtact gatgtagata
       61 ttgaagcatt tgaaaatact aataatcaga taggtttaga tttaggaatt aaagaatttt
      121 gtatctcaag ttgtggagaa tttatagaaa atcctaagta tcttaagaag tcattaaaca
      181 agcttgctaa attacaaaga gaattatcga gaaaaacaat cggtagttta aatagaaata
      241 aggcaagatt aaaagttgca agacttcaag aacatatagc taatcaaaga aatgattttc
      301 tacaaaaact atctactaaa ttaataaaag agaatgatat tatttgcata gaagatttac
      361 aagtaaagaa tatgattaga aatagtaaac tatctcgctt aatttcagat gtatcatggt
      421 cagaatttat tcgtcaatta gaatacaagg ctaattggta tggtagacaa attgtaaagg
      481 taggtaaatt ctttgctagt tcacaaatat gtaataaatg tgggtataaa aacgaggaag
      541 ttaaggactt aaatataaga gaatggattt gtcctagttg taatgaaact cacgatagag
      601 atataaatgc aagtataaat atattaaaag aaggattaag actaataacg attcaaaata
      661 aataacatat gtaagaaccg taggaactac ggggatagcc tgtggagaat tagtaagaca
      721 tattttagta tgcaaaattc tatgaagcag gaaccctgtg actttagtca tgggaggttc
      781 agtttatttc aaataatatt ttacgtaatc catctatgtt aattggatta atagcagcat
      841 ttggtcttgt actacagaag aagaaagcat ctgatgttat aaaggggtca ctacttgcag
      901 ctttcggaat tataattatg gagactggaa caggtatgtt agttagttca atagcaccaa
      961 ttaatggagc tttccaaagt ataagtggtg gtgcagtaac aaaaggttta agtgatgtta
     1021 cttttactgc aagttatggt ggaattatag gtttagcaat gtttgcgggt ttagctattc
     1081 acctaatgat agcaagattt actcctatta agacaatctt cttaacaggt cacatgcttt
     1141 attggttccc attcatattt gtagcttcag gagttgaagc tggattaaat ggtggagtat
     1201 taataacttt tgcagcagta ttatcagcac tctattggtc aataatgcct tggttattaa
     1261 gaaaatatgt gtttgatgtt ataggagatg attcttttac attaggtcat ccatctggtt
     1321 gtttagcact tatatcagga tttgttgcta aattagtagg aaataaagaa aaatctacag
     1381 aagatataaa catacctcaa agtttatcat tttttagaga aatatctata agtggtggaa
     1441 tggcagtgtt tttaatgttt atagttgttg ggatagtaat accaggtgcg tttaaagata
     1501 gtaatgagtt tattggaacg tatgcaataa agcaaggtct ttcttttgga gcaggtctta
     1561 taataatgct tcatggggta agaatgctaa taaatcaaat aatgccagca tttcaaggta
     1621 tatctgaaaa agttgtgcca aatgcactac ctgcttttga ctgtccaata ttatttaatt
     1681 ataaaccaaa tgctgtatta ataggattct tagttgctat gctaacatct acagctatga
     1741 ttttaatata taactacttt aatgtattta atttgctact tataccactt gttataactt
     1801 cattctttga gtgtggagca gcagcagtaa taggagaatc acaaggtggt atacgtggag
     1861 ctataatagg tacagctata gctgcaatat ttatggtagt tttagtagga atatctgctg
     1921 taatttactc aggaactatt caaaattgga tactaatatt tggaggtaat gacctttcat
     1981 tatttggtat aatcagtagg tttataggtt cactatttgg aggaatatta tagatatgaa
     2041 atttaaatac atagatgaga taagaagatt aattgatata atagaaaacg aagaaaaagt
     2101 caatatggaa aaagcagtac aaatctttgt tgaagcaata gaaagtaaaa atgctatatt
     2161 ttcatttgga gcttcacatg caggaatatt aacagaagaa ttattctata gagcaggtgg
     2221 ctgtgttctt ataaatccag tatttgcaaa atctctaatg ttagatacag cgcctataac
     2281 tcacacaagt aagatggaaa gattagaagg atatggtaca gctctagctg ataaaactcc
     2341 aattaaaaaa ggagatgtag ttatagttca ttcagtatct ggtagaaatc cagtaagtat
     2401 agagtttgct atgaaagcta aggaaaataa ggcaatagta atatgtataa ctaatttaag
     2461 ttactcaaaa agtgtatctt caagacattc ttcaggaaaa aaactttatg aagtatgcga
     2521 tttagtgata gataatcatg gtcaaaaggg agatgcctgt gtagagatag atggattaaa
     2581 ccaaaaagtt agtccaactt ctactgtaat agcaactact gtgatgaact ctattatagc
     2641 tcaagctact caggaattag ttaataaagg gctaaagaat cctccgattt tctatagtgc
     2701 caatattgat ggtggagatg agttaaataa gaagattttt gatgaatata aaagtgtaat
     2761 tcattatgaa tattaaggtt aaaaaaatat gttaatgaac tttgaagttt atcaacaaag
     2821 aatattaata atatataata aatgctatca tttaaaagct gtaggtaaaa atctacagct
     2881 ttttattatt gtagattaaa aagatgcgta attaaatttt aaattggaga aaataaattg
     2941 gaaggtatca attataaata ttagaaacgt tgtcatagaa aaaaactata ttaaaaaaaa
     3001 gtacatagaa aaaactgatt agacaataaa tacatgcttt gataatattt taaatggaat
     3061 gaaaatgtgt agaattataa tgaaaaaaga aaataaaaat gaaaaaagaa aataaaatgg
     3121 agggaataaa agtgggtatg ttaaaaaaat tatgttcact agctatggta gcaattatga
     3181 ctacaacact tataacaggg tgttcaagtg gaaatggaaa agattcaaaa aaggatggcg
     3241 aaaaagaaaa actagttctt tggatgccac cagtagaaaa taatatgaag gaagtttggg
     3301 accctatttt agataaattt gaggaaaaaa ataactgtga agtagattta cagataattc
     3361 cttgggataa ttattctgaa aaatttgcaa cagctataag tgctggcgaa ggtccagatg
     3421 taggatatat gtatgcagaa atgtatccac agtttataga aagtggagca gtagaagatt
     3481 tgactaacta tgcaactaaa gaggataaag agcaatctat ttatataaaa acatctgaaa
     3541 tgatgggtgg aatgtatggt atgccattcc aagcagcaaa tccaggggta ttatactata
     3601 acaaggatat tttagagaaa ctaggagaaa aacctcctaa aacatgggaa gattttaaaa
     3661 gaatttgtaa aaaagcaacg caagatactg atggtgatgg aaagatagac caatggggat
     3721 tagctcaggg atggggagct aaaacttttg gtaacttaaa ctggaactgg tatccttatt
     3781 tatggcaagc tggtggagat atatttaatg atgatttaaa atcggttaaa tttaatgata
     3841 agagtggttt agaagcagct aatttcttaa aagaattaca agcatatgtg cctgaagatt
     3901 ctttatctaa agatagtaat gaaatgatag aaagcgtatt tggaccaggg aaagcagcat
     3961 ttacaataat gttatcttct gctgcaacaa gtgtatttga tgaatcattc ccggatttaa
     4021 attggggatt tgtaactgga ctagaagata agaaagcagc aacttttgga gcagtagacc
     4081 atttatcttt aatgtcatct gctaaaaata aagaacttgc atacaagtta atacaacata
     4141 tgttaagtgt tgaatcaatg acagaatttc ataaagcaat accaagagca ccaataacaa
     4201 aaggtgaacc atatcaaggt gatgaaagat tcaaagaaat ggtggaaaat gacaaaaatg
     4261 tatatagacc attagtagta ggacctcatg gagttgaaat atatgaatat ttatggaaag
     4321 aacttcaaac tatgatttca ggagataaaa ctccaaaaca agcgttagat gatgctgcaa
     4381 aatattcaaa tgatttatta gcacaataaa agtttaaagc tgtaatatgt tgtaaaggac
     4441 tttacagatt aaaattagca ttgtatttat aaatacaatg ctaatattta tattggagag
     4501 gataaaatgg aaaacttagt tagagagttt gaagaaaatt caaaaagaaa aaaaacaaaa
     4561 ttaaatatta aaccatatat gtatatattg ccattgggaa taattttagt ggcgttttat
     4621 gtgataccaa taataatgtc aatatacttt agttttacaa agtataatat tattagtcca
     4681 gctactttta taggtttaga aaattataaa aaattattta cagatgaaac attaaaagta
     4741 tctattataa atactattaa atttactgtt gttgtagtac cttgccaaac tatattatca
     4801 ttgattttgg ctgtttggat tacaggaaaa ggaaatagta aaatagcttc atttgctaaa
     4861 ggtgctatat ttataccagt attatcatct atggttttga taggtatggt atggagggct
     4921 ttattgaatg gagaaggttc tattatatat caggtattag gtgcctttgg aatagaatca
     4981 tctaaacttt taggagatag taagacagca ttacctacac ttatgtttat atctatgtgg
     5041 aaaaatatag ggtattttat ggtaatttat atttcagcta taatgaattt accaaaacac
     5101 tgctatgaag tagcgaaggt tgatggggct acaaaacttc aagaatttat aaaaataact
     5161 gtacctttat tgaaaccaac tacaataatg gttgtatttt tagggtcgat atggtcatta
     5221 caagtatttg acttaattta tactgtgaca ggtggaggtc caggtatatc tactatgagt
     5281 atagttatgc atgcatttaa cttaaacttt aaaaacttta attctggata tgcaatgact
     5341 gtagcaaacg tattattttt gttaatagca gtagtttcga ttttacaaaa taagcttgta
     5401 agacgagaca attcagattt ttagaaaggt aggtataata tgaaaacagc aagaagatta
     5461 ttaagtgatg gtttgctttt atttatagcg tgtgcaagtt tggtaccttt tatatacatg
     5521 ttgataatat cattgaagat aacatataac tcatatagtt tggatatatc tttttcaaca
     5581 gtaacccttc aaaactatat agatattttt actaaaaaag gatttgcaca atattttttt
     5641 aatacagcaa tagtatcttt ttcaggtgta ttattaaatt tagtatttag tacattagca
     5701 ggatattctt ttgcgaagat ggattttaaa ggtagtgata agttattttt atttatgata
     5761 atgactttaa ttataccttc ccaagttaca atgataccac tatatataat aatgaaacac
     5821 ttaggatgga taaatagtta tcttgcactt attatgccaa taccaactgc atttggcgta
     5881 tttataatga gacaagctat tttaggagta ccaaaagagt tattagaatc agcaaagata
     5941 gatggttgtt cggattttag aattttaata caaatagtgt tgcctttaat taaaccagca
     6001 ttaattacac tagcaatatt tacttttatg ggagcatgga acgagtttat gtggcctctt
     6061 atagctacaa ctaaagatac aatgagaaca ttaacagttg gattatctac tcttaagaca
     6121 ttccagataa ctaattatgg acagatgatg gcaggggcta caataacatt tttacctcca
     6181 tttattttct atttgatttt acagagtaaa tttgttgaag gtgtgtcttt gtctggtata
     6241 aaaggttaaa tagtctaatt taataatata taaagaaact tatataaata tggaggaatt
     6301 taaactatgg aaatttcata tgtactgaaa gattgcacat tcaaacacaa taaatactca
     6361 gttgtagaag ctgaaacttg gaaagataag gatttggata ttgtagtagc taaaggtgaa
     6421 aaatttgcat ttcaaattat gttaaaagca acagaagaat ttaattgtac aattgataaa
     6481 agtagtgcta tatcatggaa aggcctggga aatagagtaa ggttagcatt aaatatgcct
     6541 aatagtttag aaaataattt tagtataaat atattaggat atgttcaaga tgatacaaag
     6601 gcatttgtga atgatgctat tttaagggat aaagatgtgt tagtagagca atatttgcct
     6661 caaacatttt ggattgaagg tcaagtacca gaagattttg aagaaaataa ccttgatata
     6721 ggtattgata tatttaaaag ctttggatat gaagacgaag aaaaagtttg tacaattgat
     6781 gttcctgtaa aagtaagaaa tgttgtgcta aagcctttag atgaaagcaa gttcttttta
     6841 gatttatggc aacatccaag ctgtttagct agaatgtata aagttgagtt atggtctgat
     6901 atacattttg aaatagtcga taactattta aaagaattag catctttagg agaaaaagtt
     6961 gcaacagtta tagtttcaga ttatccatgg gcagggcaaa gttgttataa agtttataaa
     7021 aatccatcaa acttatatga atataacatg gtaagtgtat caaaaggatt ggatggaaag
     7081 attaaatgta actttgagag tatggatagg tatataagca tagctgataa gtataaaatg
     7141 gctaaggaga tagatttatt tggtctatta ggtaattggt gtgctggtga gtttggaaat
     7201 cctgtagaag gttataagga ccctataaga gtaagatatt ttgatgaaaa ggataaagta
     7261 tttaaattta taaacaatac aaatgattta aaagaatata taggattagt attaaatcat
     7321 ttaatagagt gtggattatg ggacagagta aggattatag ctgatgaacc aaataatcca
     7381 gaagtagtca aagagtgtat agaatttata aattctactg taagcactca tcaagttaaa
     7441 tataaatcag caactcacga ccaaaacttt ttagacagag ctaaagatga aattgatgat
     7501 atgtcaataa atttaaaact tactatacaa aattataaag atatagaaag tcttaagaaa
     7561 aaaatcaatg acaagggtgg gatattgact tggtttgtat gctgttttcc agagaaacca
     7621 aatagttttt taagttctcc ttttgtagaa aatagaataa taggatggta tacttattac
     7681 tttggtcttg atggattttt aagatgggac tataatttat ggacagaaga cccttggaaa
     7741 gattcaagtt acaaattccc tatttggaaa gctggagata tgttctttgt ttatcctgga
     7801 aaagatttaa agccagttag aagtgtcaga atggagaatt taagatttgg aatacaggac
     7861 tttgagttat ttacaatgtt agaaaaagaa aagggaagag agtatataga agttgagctt
     7921 atgcaagaat tattaaataa aaaagaaaat gctgaaatta aaggttttgg tgacatagaa
     7981 ctaggatatt cacttgataa ttggggatat gataaagtta aaaaatatgt attagagttg
     8041 ttagacagag tataaaaaat ataagagtgt ctcaaaatga actttttagt tcatgaggca
     8101 ctctttttgt atgaaatcaa tatagtttca ttgtttcaac aatgaaaaat aggattattt
     8161 ctattctaag acagcccttt agttttttag ttgtattcaa tgttttaaaa ctaactataa
     8221 acgctataaa gtttataaat gacaataatt atatataaca tgatgtactt taatttgcta
     8281 taatagggat tgaataatat tatatggaag ataacattgt ttggggggat taatataaaa
     8341 agaataggta ttattataat gtttttttta agtatatctt ttattggatt ttatcttgta
     8401 ataagagata acagtgatac agttaagatg caacgaggga taaatatagg taatgcacta
     8461 gaatcaccta aggattttcc gtgggatgta aaaatgtcaa ataagttttt tgatgatata
     8521 aaagatgcag gttttgacac agtacgtata ccagtaaggt tttctgatta tacgtcagat
     8581 tctgataatt ttaaaattga tgaagaattt tttaaaaaga ttgataaata tgtagattat
     8641 gcattggata aagacttaat agttgtgttg gatttacacc attttgaaga aattatgaaa
     8701 gaaccaagag ttcataaaga aaagttttta aaaatatggc aacagatagc taataggtat
     8761 caaaaatatg ataaaaaatt ggtatttgaa ttattaaatg aacctaaaga aaatttatat
     8821 tcacagttac taaacgaata cattgaagaa gcaataaaga taattagaaa aactaaccct
     8881 aaaagaacta ttatagtagg accatataat ttctatcaaa ttgattattt gaatgaatta
     8941 aatattccta aagattctaa tatagtagtt tcctttcact attacgagcc aaatgatttt
     9001 gcatttcaag gaaatatata tcataaggga tttgagcact taagcaatat tacttgggaa
     9061 ggaacaaacg aacaaatgga ttatttaaaa aaaaggtttg atactgtgga aaattgggca
     9121 aataaaaata aggtaaaaat cttcttgggt gaatttggaa taacaaaaga ggcaccagaa
     9181 gcttcaagaa gagcatggat aaaagctgta agagaagaag ctgaaaaacg aaacttttca
     9241 tgggcttatt gggagcttgc atctggattt ggtatataca atcaaattga agggacttgg
     9301 gatagagata tattaagtgc attaatagaa aagaggtgat tttatgaaga aaaattcaaa
     9361 aaagatactt ataactttaa ctattattac taatgtcata tatatattgt ggagaatttt
     9421 ttacacagta cctaaagaag aagggaagtt tgccttaata tgtgccataa tacttctttt
     9481 tgtagagata atgggcatga tggaaatgtt tgtgcattat tatggtatgt caaatattga
     9541 gtaccctgaa aaaccaatca taagtgaaga actttatcca catgtagatg tattcattgc
     9601 aacatataat gaatctgttg atttggtgag aaaaactgta aatgggtgta ttcatatgca
     9661 gtatccagat aagaaaaagg tacatatata catatgtgat gatggaaatc gtgaagaaat
     9721 gtgtattcta gcagaaaaaa tgggtgtaaa ttacataact agaactgaaa gagaaggagc
     9781 aaaagctgga aatttaaata atgcaatgca acatacaaat tcccctttga ttgctacatt
     9841 tgatgcagat atgataccaa tgcatgactt tttaattgct acagtaccat actttttgaa
     9901 aaatgagcaa gctaaaaaag atggagaaaa agaggagtat gaaaaggttg gttttgtgca
     9961 aactccacag agtttttata atccagactt gtttcaattt aatctgcact ctgaaggaag
    10021 gattccaaat gagcaagatt atttttacag agacattcaa ttggctagaa ataggacaaa
    10081 ttctgtaatt tatggtggta gtaatacagt tatttcaagg gaagcacttg aagaagttga
    10141 tggattttat acttattcta ttacagaaga ttttgcaaca ggtatcttaa ttcaaagtaa
    10201 ggggtataga tgttatgcta ttccagaagt acatgcatca ggtttatctc ctacagattt
    10261 aaaaagtctt attaagcaac gtgaaagatg ggcacgtgga tgtatacaaa ctggtagacg
    10321 tctaaatatt ttgtttagga gaggtttagg tttttggcag aaaataagtt atatttcttc
    10381 aattacttat tggtatgcaa gtataaaacg ctttgtatat ataatggcac ctattttatt
    10441 ttctgtgttt aatgtgattg tagtaaaatg tacgttactt caagtattgg tattttggtt
    10501 gccaatgtat attttaagta gtttatctct taaaatattt tctcaaaata taagaaatac
    10561 tagatggacc aatatatatg aaactataat gtttcagtct ttaatgccag cagtaatact
    10621 tgaaacgttt gcaatatcta aaaataaatt ttcagtaaca aataagagta aattagaaga
    10681 aaatagaatg tataagtttt tacagggaat accttatttt atttacatgg tcttatctat
    10741 cataggaatt ttaaaaatgt ttgtagcaat atttaaaatg agttcaatga catattcagt
    10801 tgtattattt tggttgattg gaaacctttt taaccttgtt atggctacat tatttatatc
    10861 agggagacag cagttaagaa agtctgagcg ttatattgct gaaattgatt ttaagttaaa
    10921 gcaaaattct tatgttcttt cttcaaaaac aattgatata tcagaaaatg ggtttgcatt
    10981 tttgctagaa aatccagaat atatttcccc agaagaagag tttgaggtag agtttagaga
    11041 aaaatctgga aatgaaatgt atatagctaa tatgaaagca aagattgtta atgttgtgga
    11101 agtaaattca aagtggaaat atgcagctta tattacacat attgaagata gtgaaataga
    11161 taattggatg tgtattgttc acgatagaat acctacttta ccaatgacaa tatctaatca
    11221 actaggtttc tttgatgatt tgcaaattaa tgtaaaaaaa cgtattgaaa aaactagaac
    11281 attatcacga agaagtccaa gaatcaatat gaattttcag atggatataa aaaatacagg
    11341 taaattaaga atagtgaatt ttaattatca atatgtatta ttaaattttg agaataaaag
    11401 tatttatcct aaagaaatag cattggaaat taatgaaaat attgtgttag aatgtgattt
    11461 gtgtgagggt aaaattgatg aaagaggtat cttatataaa gtaaataaca ttgacagcat
    11521 aatgcaaaat ttctttttaa gagatgaaat gatggattgg atattacaaa ataagaaaat
    11581 tcttgattca aagcctagtg aaaagaagga aaaaagtata gatgagtttg aacccatgga
    11641 atatatttag gagagatgaa atgaagactt taaaagtgtt tttttgttct acaaaatatc
    11701 ttattttggt gtttattttt tttataaata ctacttttat aagttcagca acaagtgata
    11761 aaattttaga tttatccttt aaaaagatag agactgactt aagttctaag attacatatg
    11821 aggatacagg tgtaaaaata gaaacggata gttctaaaag cgataaggaa cgttatttat
    11881 atatttacca aaatataaaa gaaaattgga gcatgtataa taatttttat attgaaattc
    11941 aaaataaaaa taaaagttct cagagaataa atttaagcat acaaagtaaa aatatgcttg
    12001 aatttagatt aaaagaaggt agtgaagtat ttttagaagg taaaaatata atatattcag
    12061 ataaaataaa agaaggatgc atagaagtac caggtgaatt tgaaggcaaa atttatgtga
    12121 actttaatag ccttataaat gaggaaagta atgttgtgct ggactcaaat atgctttcaa
    12181 acattgttag ttggggaatt acgtttattc catctgatga agaacataat attgttatta
    12241 taaaaaagat atctttattg tctgaagaga aattaagatt tttaaataat attaaaataa
    12301 ttggcgatga agaagtacaa ataccagtat tgggtcaaag tataagtcag tatgaggtat
    12361 taggtcttaa aagtgattct aaaattaagt actctcttat gggaaaacaa gataatgtga
    12421 gtatatctca aaacggtaaa ttaacattaa gtaataaagc caaacctgga caaattattt
    12481 tacaagttaa tgttgatgat aagtttaaaa ttggaaagaa aataacatta actgaatctt
    12541 ggagtattaa taaaaaagat aaggatggtg ttccatatac attagtatct cctgagcaat
    12601 caccaacagt acaagatatg aaaaaaataa attttatgaa taatatcata acgtttgttc
    12661 gaattttatt tgtatctcta gtaataatat gctttggaat atatttatat tggaaaaaat
    12721 gttcaaaaac taagtaaaga gaggaggtta gattgtgtta ttttcaagtt taattttttt
    12781 attttatttt ttacctataa ctttggtttt atattatgta tttaggttta accgaacgat
    12841 acagaacatg atattgcttg ctgttagttt atttttttat gcttggggag agccaaaatt
    12901 tgttgttatc atgatagttt ccataataat gaactatata tttggtttac tggtagatag
    12961 atatagggaa agtaaaataa aagtaaaagt ttttttagtt ttgatgtgtg catacaatat
    13021 aggtgtttta tttatattta aatatctagc ttttgcgcta agaaatatta gtgaacttat
    13081 tagtactgaa ttgactatac ctaatattat tttacctata ggaatttcat tttttacatt
    13141 tcaaggaatg tcttatgtta tagatgtata tcgtagacat ggagaagtcc aaaagaatcc
    13201 tttttatgtg ggactgtata tagcattttt cccacaatta atagcaggtc ctatagttag
    13261 atatgagagt gtggcagaac aaattctcaa tagaaaagaa acttggaata aattttctat
    13321 tggtacttgc aggttcataa caggtctagg taaaaaagta ttaatttcaa ataacatggc
    13381 tatagtagca gactatattt acactatgaa tagtcaagga gaaatagctg catcacttgc
    13441 atggcttggt tcaatagcct atactctgca aatattcttt gatttttctg cttattcaga
    13501 tatggctata ggcttaggtc ttatgtttgg ttttaaattt gaagagaact ttaattatcc
    13561 atatatatca aaatcaatta gtgagttttg gagaagatgg cacatttctt tgggaatgtg
    13621 gtttaagagc tatatatact ttccgcttgg aggttctaga gtagcaaata aagatattat
    13681 gattagaaat atgtttatag tgtggttatt cactggaata tggcatggag cagaatggac
    13741 atttgtaatt tgggggattt taaactttgt tttcctaatt atagagcgat ttataatgtt
    13801 tgaaaagatt gaaaatcaca attttataaa acatatttat gctttattag tagttaattt
    13861 tggatgggta ttgtttaggt cacctaattt aaaagaagca tataattatt ttaaagctat
    13921 gtttggaggc aatggagcta tatggagtga ttatacatat atgtttttaa aagaatactg
    13981 ggttttcttt atatttgcat ttatttttag tgtacctatt gcaaagaaaa tcaataagtt
    14041 tgttgttgaa caagctaaat atagtatgat atttaacgta ttttatccaa tctcagctat
    14101 tttattgttt tttatttctg tgacatattt agtaactgga agctataatc catttattta
    14161 ttttaatttt tagttgggag gaaaagtatg aatagatttt ttaaagtaag attaataact
    14221 gcaattatgt tcttaattat tttattcgct ttctctatct ttaatgtttg ttattctgga
    14281 aaagatacga tacaggaaat aaaaaattca atagctgaaa aaagtagttt aaaagataca
    14341 atatcatcta ttgataatac agtgaatgac aaagtaatat acaaaaatac gttcattgaa
    14401 acttatggct atttacaaaa attaatgggt aaaaatgagt tttcaaaatt tagtgttgta
    14461 aaagatactt ctggaaagat gcattataca tattttgcta aagctccaaa tccagttggt
    14521 gtgatagcag atagggtaag aaaattttct gatgaaatgg aaaagcaaaa cactaaattg
    14581 gtttacctaa tgactccaga taagtatata aaaggagtga caaaatttcc aaaaggaata
    14641 ccttattctt atagtaatga gacagcagat aattttttaa atcaactaaa aaagtataat
    14701 gtggactata ttgattttag agaaaatatt ttaaagagtg gtattccaaa agatgattta
    14761 ttttttaaaa cagaccatca ctggaaagta gaaacttctt tttgggcatt tggagaatta
    14821 gttgagcaac ttaataaaaa atacaatatg aatttagatg aaaatcatta ttatagagat
    14881 aaggacaatt ataattcaat tgtatatcct aagtcatttc ttggttctat gggaagaaag
    14941 actggtatct tatatggtgg tatagatgat tttacattaa tttatcctaa atttaagact
    15001 aattatactt attatacaga ttctaaatct caaaaatttg aattaaaagg aaggtttgaa
    15061 gagagtttaa tattatcata tccttttaat gcagaccttg atttaatgga tggtcaatca
    15121 gataagtatt ttacatattt attggggaac agaccattag ttaaaataaa aaatatagaa
    15181 aatccagatg gattaaaagt gctgtttgtt aaagattctc taattgtacc aacagcagca
    15241 tttttttcct ctgtttgttc atctatagat atgatagacc ctagatatta tgatggagat
    15301 atactagaat atgcaaaatc ccatgactat gactttgttt ttatatctgt atatccccaa
    15361 aatcttacaa aagaattttt tccgttttgt gagtaaattt ctaaaaaagt tttctgataa
    15421 aataaatggc taactggtaa aaagttagcc atttattttg agtgttattc taagaaatat
    15481 tttttttact aaatacttct ttatctattt ttccaagtga ttttcctaca gtaattgatg
    15541 ataccatagc accattaaca tttatcatag tacgtcccat atctagtata gggtctattg
    15601 caagtatacc accaactaat ggaaaataag aaccaagacc aactccagag ataacaactg
    15661 aaacggccat tgtagcagtt cctggaagac cagctattcc aaacgaacta attgttataa
    15721 caattaacaa cataacataa aagctcatat ccatgtttac tccagccata ttggcaagtg
    15781 ttacagccat taaagcagga tatatacctg cacaaccatt catacccata tttgcaccta
    15841 aacttgctgt aaagcttgat acaccctcat caactccgtg attttgtact agactttcta
    15901 tcgttacagg caatgttcct aaactagaac gtgaagtaaa tgccagaagt aaagcttcag
    15961 ttgaattttt tatataagta atgggattta gaccatttaa agttataaaa attaaatgta
    16021 taataaataa tattgcaact gatatataaa gagctaatat aaactgtaaa acagatacta
    16081 ttgaactaat accttgtgaa gttattgtat ttgcaagcag agcaaccaca gcataaggca
    16141 taaatttaat aacagtcata gacacactta caattatttt gtaagatgct tctataaaat
    16201 ctataaatgg ttttattgtc tcaaaatgtt ttttacttaa tctctttatt gaggtagcta
    16261 taaatgtagc aaatattatt gtagctacaa tatttccatt tgccatagcc attacaggat
    16321 tagatggtag taatcctctt aaagtatcca caagagagtt tatttcttta agttcttgat
    16381 tttcaactaa agtaggcata ttagctccta cacctaactt cattatattt gcaacgataa
    16441 ttccaacaat tgcagctata gcagtagtag caagaagcat acctaaagat ttaaatgtca
    16501 gagttccaag attgttatct ttcatattta taattacttt tataatggat aagaaaacta
    16561 aaggtactac taacattttt aataagtcca taaatccaga accaaatagg ctataccact
    16621 tacttacttc ttgtagccaa gtgacttgag ttgtatcatt aggaaaacca gcaaaagctt
    16681 gtattatgat tcccaatatg agtcctagta ctgtagcata tattgttctc aatgaaaatt
    16741 ttattttttt cttttcaaat tgtctaacaa taaagaacgt acctagaagt aatattatga
    16801 aaaaaattgt tttaaaatct gtaatcataa gaaattgtga taaaaatgaa ctttccaata
    16861 tttttctcct tttatttaaa aataaatttt tctaagttta aatgatatca atatattaaa
    16921 aaaatgtcaa tattatatca caatttactt aaatagagta aaacaagttg attttgaaaa
    16981 atgtgattaa gtacagaaaa atagggtata ataaatgttt aaagaatttg aattgtggta
    17041 agaaaataaa aaatagcttt tataagattt gatttcaaat taaaatatgt taaataaata
    17101 actaaaaatt attataaaaa atgttttcat agaatatata tcaaatgaaa agcttaaaat
    17161 aagtgaagaa attaactaga catcaaagga gagataaaat gagagtaata ataattggtg
    17221 gtgttgcagc tggtatgagt gctgcagcaa aattaaaaag aataaaacct gagtatgaag
    17281 ttgttgttta tgaaaaaact gaaatagtat cttttggtgc ttgtggatta ccatactttg
    17341 ttggtggatt ttttaatgat gctgatgaat tattagcaag aacaccagag aaacttagag
    17401 aagctggaat tgatttaaat atatttagag aagtcgttga ggttgatagt gaatctaaga
    17461 aaataaaagt aaaaaacata aaaacagatg aaatatatga agattactat gataaattga
    17521 tgatagcaac tggagcaaga agtattatgc ctcctataaa aaacataaaa ttgaagaatg
    17581 tttctacatt aaaaagttta tatgatggag aatatctaaa aaaactttta agtaatgaag
    17641 ataataaaag agtgacaata ataggagctg gttttatagg gttagaagca gttgaggcat
    17701 gtaagaaatt aggtaaagat gtagatgtaa ttcagttaga agatagaata ttgccacaag
    17761 tatttgataa ggaaatgacg gatgtattag aagaagaaat aaatagacat aatgttaatt
    17821 tgcacttaga tgaaatggtt gtagaacttt gtggagaaga taaagtagaa aaagttataa
    17881 caaacaaagg ggaaatagat acagatgtag taataattgc tactggcgtg agacctaata
    17941 ccgagttttt gtcaaatact aatataaaaa tgctaaaaaa tggagctata gtagttgatg
    18001 agtatggaag aacttctgta gaagatatat actctgctgg agattgtgca actataaaga
    18061 atatagtaag taatgaaaat gtatatgttc ctttagctac tggagctaat aaattgggta
    18121 ggatagtagg agaaaaccta gcaggaagag aagtatctta tcaaggttca ctatcttcaa
    18181 gctgtataaa aattatggat atggaggcag catcaactgg aattacagaa aagcaagcta
    18241 aagatttagg cataaatgtc aaatctaaat ttatttctga ttataatcaa actcattatt
    18301 atccaggaag aaataaaata tatgtaaagc ttatttatga tgcagataca aaagttattt
    18361 taggcggaca agtggctggg tttaaggatg ctgtccaaag agcaaatgta ttggcagctg
    18421 ctatctttgg aaaaatgacc acaaatcaac ttggaatgtt agatttatgt tatgctccac
    18481 catttgcaag aacatgggat gttttaaatg tagctggaaa tgtatctaaa taatatttag
    18541 attatagcta atatagttga caagcaatta agggaatgaa tttacctaaa ctatttttat
    18601 catggtaaaa ttcattccta aatttttatt ccaaattttt attccaaatt tttagaaata
    18661 aggattccat taaattattc atcaaaattt aaacgcaagc ttaatctatc ttctccaagg
    18721 atagtatttt caaaaacatt agtagcattt tctaaataca tactaggtat gtctaaagaa
    18781 ggactaaaat gagtcagtaa aagttgtttg acatttccaa gttttgcaag attagcagct
    18841 tctctaaaag tcatatgttt gtttctaaca gcttttgata tatctaaatc atcaccatac
    18901 atggcttcac atataaataa atcactatct tttatgaaat ctggtattga ttctataggt
    18961 cttgtatcgg ttgtaagact tatcttaact ccctttctca attctcctaa gaccatatct
    19021 ggagtgtatt gttttgaatc taaaactata ttttgacctt cttggagttt gttccataat
    19081 attttaggaa cttcgttttt agttgcctta tctatatcaa atttagggtt tcttctaaaa
    19141 ttaaaactgt atcctaaaca ctctatagag tgttctaaac tgattgttga aatttctaaa
    19201 tttcttaaaa ctttattatc taaagaaaaa ttgccttgag ggttttctat aatttttaaa
    19261 gtgtatggta catattctac taaatttctc atagaacgta tacaatcgac tattccaact
    19321 ggaccaacta tagttaaatc acttgtacga ccactatttc caattgttga taaaagtcct
    19381 aaaagtccaa atatatggtc accatgtaaa tgggttatac aaatcaagtc tatatcttta
    19441 aagccacatt tttttagctt catagatacc tgagtacctt caccacagtc aattagtatt
    19501 tttctacctt tatagtttat aaatacagag gatagaaatc tgtttggcat aggtacattt
    19561 ccaccacatc caagtaaagt taaatctatc attttttccc ctttctaaaa ttaacacaaa
    19621 tctgttgttt gtttttatta tgatatccta ttataattat tttaacatta aatataacaa
    19681 taaatgtgta tttgagtcaa gaaatattgt caaataaagt ttttatatag aaatgtataa
    19741 aaatttggaa aacaaaaagt gaaaattata tttaggagca tttaaaggag gaaaatttaa
    19801 tgaaagtatt aaagaaaatt actattttgt cattaattac attaacctta gttggttgta
    19861 gtaacggaga agataaaaaa gtggttgaag gttctaagaa aattcaaaat tctaaaaaag
    19921 atgaacagcc tataatagta cataaagatg caaatatatt gaaagatagg tatgagccac
    19981 caaaaggatt taaaagagtt gatgtagaaa aaggtagttt tgaatcattt cttaggaata
    20041 ctaagctaaa aaattatgga gagaaagtaa agtattatga tggaagaact aaggaatcaa
    20101 aaggtattta tgatagtgta tttgatgttg atattggcaa tagagatttg catcaatgtg
    20161 cagatgcaat tatgcttatg agagcagaat acttgtatca gaataaacta tatgatgaaa
    20221 taagttttga ttttgttgat ggttttaagg cgaagtattc aaaatgggca caaggatata
    20281 gaatttcagt aaaagatagt ggttcaagct ggtataaagc aactgaatat tcaacatctt
    20341 atgagagttt tagaaagttt atggatatag tgtttgcata ttcaggaacg ttatctcttg
    20401 aaaaagaatt ggagcctgta aaagttgagg atatgaaaat aggagatgtg tttatagttg
    20461 gtggaagtcc aggacatgca agtataatta tggatatggc agaaaatgaa aagactggtg
    20521 aaaaggtatt tatgatagcc caaagctata tgcctgcaca acaaacgcaa cttcttatta
    20581 atcgtaaaaa tccaaatttg agtccttggt atagtttaga ttttgaaggt gatttgacaa
    20641 tacctcaatg gacttttaaa agagaccagt tgaaaagatt ttaaaataat aattttatat
    20701 aataaaagaa aaatatagat gtgaaaaaat gtaattagat taaaactaat tggatatgga
    20761 caaaattaaa ttgatagata aatattaaaa attacactaa ttaatcttac tatagtttaa
    20821 tatttatcta aaaagtttta tgtaaaatat aaattttata ttaaagctaa aactaatttg
    20881 aggaggctaa tttatattga agattagaag gtatttaatt ttggtaatta taattctaat
    20941 tacaacaagt cagagtttta tttcttatgg gctagagcga tttgaagaaa taaccatttt
    21001 tcacactaat gatatacatg gaagatatgc tgaaggtgat gaccatatac aaataggaaa
    21061 tctagccaca ttgaaaaaag aaactccaaa ctcaatttta gtagatgcag gagattgttt
    21121 gcatggactt ccaatagtaa atatggatag agggaaaagt gcaattgaat taattaaatg
    21181 tgctggatat gactatatta ctcctggaaa tcatgacttt aattatggaa aagatagact
    21241 gtttgagtta tctaaaagtg ctaatttagg agagaatact ttaaaatttt tatcatcaaa
    21301 tatttttgaa aatggaaaaa aagtttttga tagtaatgat ataaaagaaa tagatggaat
    21361 aaaaatagga ttttttggac tctcaaccca ggaaacaaag agcaaaactg gatttaaaaa
    21421 tgtggaagga ttagagttta aaaatcctat agaaagtgca acagaacaag taaatgaatt
    21481 aaaaggaaag ggtgccaatg taatagttgc aatatgtcat attggtacaa atagtttaag
    21541 taagccaaat agtatagata tagcaaatga ggttcaagga attgatttga ttattgatgg
    21601 acacagccac actaagtttg aaaatggtaa acaagtgggt gatacattga ttgtaagtgc
    21661 aggacaatat ttagaaaata ttggcgagat aaaactaact ttggatacta atgatgtaga
    21721 aaatataaaa atagaaaata aatcagttag attaattgga aaagaagaag cattaaagta
    21781 taagcttgac tccgctgtaa gtaaaaaggt tgatgaaata aaagaacaac aagaaaaaat
    21841 attagataaa gttatagggg taactacaaa tactctagat ggttcatatg aaaatgtaag
    21901 gacaaaagaa actaatttgg gtaatttaat aagtgacatt ttactagata aaactaaggc
    21961 tgatatttct ttatttaatg gagggaatat tcgagatacg atagaaaaag gcaatataac
    22021 aagaagagat attgtagatg tatttccttt tagcaatact atagttacca aagaacttac
    22081 aggagcacag attaaagatg tattggagca tggagtaaaa ctttatccag aaaaaagttc
    22141 agcctttttg caagtgggag gaatttcata ttactttaat cctaaacaaa aagaaggcga
    22201 aaaaataaca aatattcaaa aagaaggaaa gccattagat ttaaataaaa agtatgtagt
    22261 tgcaacgaat gattatatag catcaggtgg agatgaattt ccttgttttt caaaagagcc
    22321 agtactaaag gaatttggaa atttagagag tgcagtcatt gaatatatag agtacaaaaa
    22381 agaaatatca aaaaatgtag atggaagaat tggtattaaa tctaaagatg agttaataag
    22441 caagaatcat gagagtgatt tgatattaag tgaaaatgaa aaaaatatca atgataatga
    22501 agagaatcta tctaataata taaaaagctc tgatagtgat aaaaataaaa gcttagataa
    22561 aacaagtgct agagaaagtt ctaatctagg aatatctaaa agagaagcta aagaaaaatc
    22621 tcctaaaaca ggagatttag gtttttcaaa tagtataatt atatttatag tttcaagtac
    22681 attgatatgt ttattgaatt ttaatcaaaa agagttaaag gataaaaagt caaaataaaa
    22741 attaatgagc aaaaattagt agatagtaat ttaattttag ataatagtaa acagcaaa
//
LOCUS       DAMKMI010000051        22680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMKMI010000051 DAMKMI010000000
VERSION     DAMKMI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.052"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            27..1463
                     /locus_tag="PC429_001844"
     CDS             27..1463
                     /locus_tag="PC429_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF3847351.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            complement(1555..3429)
                     /gene="asnB"
                     /locus_tag="PC429_001845"
     CDS             complement(1555..3429)
                     /gene="asnB"
                     /locus_tag="PC429_001845"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF3847352.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            3632..4306
                     /locus_tag="PC429_001846"
     CDS             3632..4306
                     /locus_tag="PC429_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF3847353.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            4419..4955
                     /locus_tag="PC429_001847"
     CDS             4419..4955
                     /locus_tag="PC429_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF3847354.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(5121..5741)
                     /locus_tag="PC429_001848"
     CDS             complement(5121..5741)
                     /locus_tag="PC429_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF3847355.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(6055..8193)
                     /gene="vanT"
                     /locus_tag="PC429_001849"
     CDS             complement(6055..8193)
                     /gene="vanT"
                     /locus_tag="PC429_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF3847356.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            complement(8211..9017)
                     /locus_tag="PC429_001850"
     CDS             complement(8211..9017)
                     /locus_tag="PC429_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3847357.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            complement(9014..10114)
                     /gene="vanG"
                     /locus_tag="PC429_001851"
     CDS             complement(9014..10114)
                     /gene="vanG"
                     /locus_tag="PC429_001851"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF3847358.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            complement(10375..11457)
                     /locus_tag="PC429_001852"
     CDS             complement(10375..11457)
                     /locus_tag="PC429_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3847359.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            complement(11507..12208)
                     /locus_tag="PC429_001853"
     CDS             complement(11507..12208)
                     /locus_tag="PC429_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF3847360.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            complement(12642..15173)
                     /locus_tag="PC429_001854"
     CDS             complement(12642..15173)
                     /locus_tag="PC429_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3847361.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(15281..15898)
                     /locus_tag="PC429_001855"
     CDS             complement(15281..15898)
                     /locus_tag="PC429_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF3847362.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            complement(16257..17141)
                     /locus_tag="PC429_001856"
     CDS             complement(16257..17141)
                     /locus_tag="PC429_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF3847363.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(17334..18584)
                     /locus_tag="PC429_001857"
     CDS             complement(17334..18584)
                     /locus_tag="PC429_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3847364.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNIDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            complement(18806..19570)
                     /locus_tag="PC429_001858"
     CDS             complement(18806..19570)
                     /locus_tag="PC429_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847365.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            complement(19564..20289)
                     /locus_tag="PC429_001859"
     CDS             complement(19564..20289)
                     /locus_tag="PC429_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847366.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(20291..20659)
                     /locus_tag="PC429_001860"
     CDS             complement(20291..20659)
                     /locus_tag="PC429_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3847367.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            complement(20835..22379)
                     /locus_tag="PC429_001861"
     CDS             complement(20835..22379)
                     /locus_tag="PC429_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF3847368.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKRNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
BASE COUNT         7817 a         3814 c         2371 g         8678 t
ORIGIN      
        1 tatcatctta ttataggaga aaaaatatga tttgtactat taaaccatta aagatttcaa
       61 taaaaaagtc tactgaacct ctaattgaat ttatatctga tactacctat aaaaataagc
      121 gttcttcata tattaaagaa gtctacttta caagatatat aggtgttgat gattcaattg
      181 ataaatacat agaaaaaatt aaaaatatag atacttattt ttttaataat cctacaattc
      241 cctatattag attaaacaat ctaaaaataa attttgacaa agaacaaaca gataagatgc
      301 ttaaaatttt taatatttat aatagttcta agtttaacag ttgggattta tataatatcg
      361 actttccatg taaaatagaa aatgacacct taaattggac taaaaagatt gcatttaaaa
      421 atactttaga tttattttcc acaagtatgc caaattgtac atcctctatc atcaaaaact
      481 ttgcagttaa acttttgtgt tggatagact attttctacc taaattattt tataacaagg
      541 tgaagactac tatttcacca aagatagtat actttggaaa tattaaaagg caagaacttt
      601 ttttcttata ttttttatca caacttgggt gtgatatttt atatataaat ccaaataaag
      661 atattcttga tttatatcct gaatgttcaa aattttctac tctatttgaa ctttctagga
      721 agactccaaa tatattagaa ataccttttg aaaggcatat tacaaatata aattacagtg
      781 aaaataccaa taagacttct cctactacga ataaacctaa ctctaaaaat acacttaata
      841 acacaaatct aacatctaaa aaaattgtac aagatgagga agtagaactt tcttatgaag
      901 accttgcaaa gttatcagct tctgttgtta tgattgttgt gtgcaataac gaaaataaac
      961 cgtttaagag cggttctgga gtagtaataa ataatgaagg ttatatactt acgaatcttc
     1021 atgtagtcaa tgatgggtat tcatttttag ttagatttga aaatgatgat aaagtttata
     1081 catcttacca aattataaaa taccattcag attatgattt agctgtgata aaggttgata
     1141 gaaaatgcaa gccaatacct gttaaagtca gtaaaaaacc tgtgagaggt caaaaaatag
     1201 ttgcaattgg aagccctcta ggtcttttta atacagtttc agatggaatt atttcagctt
     1261 ttcgtgattt tgaaacggtt caaatgatac aatttacagc tccaatctct agtggtagtt
     1321 caggaggagc tcttttagat atgtttggaa acttactagg attgatttcc gctggatatg
     1381 atgatggaca aaacttaaat ttagctgttg aaagtaatct tgtcaaaata tttgccaata
     1441 attttattga gatagtaaac taatcaatca aaattctact tatcctattc cataataatc
     1501 aggagggtga aaacattgca tgcttttgcc ctctgattaa tattgaaaaa agtattaata
     1561 ctcgacattt ttatgctcaa taaataattg atgccataga caaaatgtaa atatagtcca
     1621 tatttttctc gaattatctc tatgaccttt aagatgcgta tctaaaagtt ttattgcata
     1681 ttttttatct ataaattcat ctacatttga attataaata gtttccttta caatatcccc
     1741 tagagaatct tttaaccaaa ctcttattgg agttggaaat cctaattttt tcctttgaac
     1801 aacatgttct gggactattt ctctaaacgc ctctctaagt aaaacttttg tattattctt
     1861 atttattttt tgctccattc ttaaattttt tgctacatca aatacatctt tatataaaaa
     1921 tggtactcta agttcaacct gctcagctgt agaaagtttg ctaatttttt gaagtatatc
     1981 gccttgaagc catgtattca catctatatg ttgcatttta cttatataat cgtaattatt
     2041 tttttgtgca tctctataca aattagacaa caggtattca tatatattct tttccttata
     2101 cttaaaaaag aatcttttta cttcgctgtt ttcaaaaact tttgcatttc ctatatatct
     2161 tttttctaat ggtgttgtag ctctataaag ataatttctt ccttttatgt ttggcataag
     2221 ttctgaaact cttcctaata tacttttaat ataagtaggt gaattgacaa ctgatttcat
     2281 agtactatac tctttatata tattatatcc tccaaataat tcatctgctc cttctccaga
     2341 caatgctact ttaacatgtt tacctgcctc tttaattaag aaataaagac ctacttctga
     2401 agggtctgct atagggtcat ccaaagaata aattacattc ggtaatgatt ttatatattc
     2461 atcttgtgtt atattaatct ggatattttc tatacctaac ttatcagctg ttttctttgc
     2521 tacttctaat tcactatatc ctttgacact aaagcctgca gaaaaagatt ttatatttgg
     2581 attaatttgt gaagccacag tagttataat agatgaatct attccaccag acagaaatgt
     2641 tccaatttct ttctcctctt ccatatgagt tactattgaa tctactacta tatttcttat
     2701 atcatcctcg ttcacatctc tattgcctaa gtgattttta gtataaaaaa attttgtact
     2761 acttctaaac tcaagtttat tatatctctt taaagatagt ataccatttt ctaccctaaa
     2821 atagtgacct gctggtatca gccttattcc ttgtatcata gtatcttcag gaagtacata
     2881 ttgaaatgaa aaataacttt gtaagcttct ttcatttata tttaaatttt tcatatattc
     2941 aagtaaaaac ttatactctg aggaaaaaat tattgcattt tccttattta tataatagag
     3001 tggttttatc ccaaataaat ctcttgctcc aaatatactt tcattttctc tatcatagat
     3061 tataaagttg aacattcctt taatcttatg tacacaattc ttaccaaatt ctatataaga
     3121 tgccaataag acttcaatat catcatttgt agtaaaattg tgtccttttt taattaattc
     3181 ttcttttatt tcatgatagt tatatatatt tccattaaaa acaataactt ttccatcttt
     3241 ttcaaatggt ttatttccat aaatagcatc aattacatta aatctttcaa aatcaaaagt
     3301 tttttcctca tccttatact ctattctact agaaagtctt gtattattta cagattcttt
     3361 tattttaaat ttaatatctt cttttttaga tatatcacct ttataataaa ttgaaatatt
     3421 gccgtccata taatcaaaat tcctttctgt cctttagtct tattctatat ttcatagttt
     3481 tgtctttggc tttaaaactt attcaattta aaatgaaatt gctattaaat tcttataaat
     3541 tatctgttaa tatttcatat tttttttatt tctagaaata ctatattagg aggtgatttt
     3601 tatgaagaaa aaaatattaa taccagttat tatgtcttta tttataatct cacagtgcat
     3661 aacttcattt gcttttacac ctgaaaataa caaatttaag gttaaaccat taccttatgc
     3721 atatgatgca cttgaacctt atatagataa agaaacaatg aaactgcatc atgataagca
     3781 ttatcaagct tatgttgata aattaaatgc tgctcttgaa aaatatcctg agctttataa
     3841 ttattcttta tgtgaattat tgcaaaattt agattcttta cctaaagata ttgctacaac
     3901 tgtaagaaat aatgcaggtg gagcttataa tcataaattc ttttttgata taatgacgcc
     3961 agaaaaaacc ataccttctg aatctttaaa agaagctatt gatagagact ttggttcttt
     4021 tgaaaaattt aagcaagagt tccaaaaatc tgctttagat gtctttggtt ctggttgggc
     4081 ttggcttgta gctactaaag atgggaaatt atctattatg actactccaa atcaggatag
     4141 ccctgtaagt aaaaacctaa ctcctataat aggacttgat gtttgggagc atgcttacta
     4201 tttaaaatat caaaatagaa gaaatgaata cattgacaac tggtttaatg tagtaaattg
     4261 gaatggtgct ttagaaaatt acaaaaattt aaaatctcaa gattaattaa atattaaaat
     4321 ccttttgaaa aaaacttatg tttgctatat aatataataa aagatagtct aaatttacca
     4381 atataatgtt tatagtgacg attcaggagg atttttatat gataaaagaa acacatgaaa
     4441 ggggaggtta tatattcgca cttttgatac tcccctttat aaacaatctg tacctagtta
     4501 aatacgatat tgcatataaa atagtattaa ttataattta tatttatttt gcatatttag
     4561 gttctttatt tccagatata gatatgagag gttcttacat aagtaaaaaa tttatgttaa
     4621 tatacaaatt atttggaagt aggtttagac atagagggtt tactcacagt ctaattgcct
     4681 tattacttat ttatagtttt tttaaatctc tgacaatctt cactaacaat aatatagttt
     4741 tctcatgttt gtccagtggg tttataatag gatatctatc tcacttgtgt cttgacctta
     4801 ttactaaaga aggtattgaa ttgttttatc ccattactat aaacatttct ttactaccta
     4861 taaaaactaa ctcaaaaaca gaaaaattca tatctaagct attaaatttc atagttattt
     4921 ttttgattgg atatcaattt tatattttat tatagttgta gaaaaaataa ataacaatat
     4981 tttgaactaa gtataaataa gttttatata ttttagttta caaaagaact aaaaattttg
     5041 taccttaata caaaaaataa aacgtatctt aaaataattt agtactaaat cattttaaga
     5101 tacgttttat ttcgtacaag ctaaatcatt ttatcatctt tatcattgtg atgtttttta
     5161 tgatggaaat cataattatg attttcaaga tttttattta ctctatttcc aatcttccat
     5221 gacaaaaaca ccaataatat aaatgatgct atctttaaag gacttgttat aaaaccttct
     5281 atatcacttg ccacgtaact tataactata aacattacaa attttcctgc caacattgct
     5341 ggcacaaaat cttttaaatc ttttttacca aaagctgatg cgatagtaac taaacaaccg
     5401 ggcataaatg gacaggcata cgctataaat aataacttaa atccctgttt atccatccaa
     5461 cttattgctt tttcagtttt actatttctc aacttattaa aaagcttact atcattaaat
     5521 ctgcttatta ataagaataa tgaagttgtc ccaagtcctg agccaatcca tgataacaaa
     5581 catccaataa ataatccttg tattgcagca tttgctgtaa ctatagcaac aagtggtaag
     5641 gctggtataa agctttctat aaatgcactt aataagccga ttattgtggc taacacccaa
     5701 taattttgag tcaaatcaag tagttcttgt atatactgca tattgctttt cccctttttt
     5761 aactttaact attattatat actctatatg tttattatat accaatctca aaaaatgaat
     5821 ctaatattaa aaagtaaata atctatataa gtttacagtt aaggtataat tattttatta
     5881 tatattataa caaaaaaggt gatgtttcaa aataaaaatc tgaaacatca cctttttata
     5941 acttaaattg tcatgtaagt tataaaaaat atatattaga cataatatta ctaaaaatgt
     6001 ctagtcagag taaaataaat tacacagtac aacaaatatt actatttaat tgagttactt
     6061 ctttataaca tttaaccttc ttcctactct gctcaataat tcattcgata tactacatga
     6121 gttatttgca acttcagaaa cagataattc agataaatta tctcttccta ttattattgc
     6181 cgtattacca acttctacat taggtatatc tgtaatatca acagcaagtt ggtccatgca
     6241 aattcgaccc actattgctg ctctacaacc atttattatt acgtaacttt ttccacagga
     6301 taaatttcta ggtataccat cggcatagcc aatagataat atagcaatta cgctatctct
     6361 gcttgcaata aagactcttc catatccaaa actatcatct ttttgtattt ttctaataag
     6421 aattacttgt gattttaagg ataaaacagg tcgtaaatca agttgcaatt tagtcgtatc
     6481 atttggaaaa cttaatatcc cgtataatgc aattccaatt cttacataat tacacttaag
     6541 attgggataa ttaaaaaaac cataactact ttgaatatgt actttaggaa tttttatacc
     6601 tttttttaac agaaaatcaa ttaacttata aaaacattca atttgtttat ttgtaaagtc
     6661 tacatcttca tctttcagac tatctgaaac acacaagtgt gtaaatatgc cacatatatc
     6721 taagtatttt aatttaaatg cctttaaaat tgacctcaca tcattaacat caaaacccag
     6781 tctgtgcata cctgtatcaa ttttaatgtg tgattttatg ttgtatttct gtttgtttaa
     6841 agagatagca tgattaaggt caataacact ctgtgtaaga ttgtatttat gaagttcctt
     6901 tgcccttatt gggttagtat atccaagaat caatatttct cctagtattc catatcgacg
     6961 taaatcaatc ccctcatcta ttgttgctac tgcaaatgct tttacaccta tttggtttgc
     7021 acaagtcgct acttcaaaag cactatgacc ataggcatta gcttttacaa ctgccatcaa
     7081 ttcacaatct tctggcatag ctttttgtat catctttaca ttatgttcca aattatttaa
     7141 atcaatttca atccaagctc tattggtatt taccttatgt tctcctttca agtctttttt
     7201 tagaggttta agaatcaata taataattat agaagctata aaagatgcta tacttaccac
     7261 tatataatga ataaggctat tgtcaaccaa gacagtttgt attccgaata ttttagaaaa
     7321 catacgaata acaacaatca tcataggatg tataatataa ataattaatg atgatgttcg
     7381 caataaccta gctcgagaac ctttcaaaaa tgttaaggct gtaaataaga aatacataca
     7441 tggcaataac ataatataca tgctatcatg acgctgtacc cctagtttat gtaaaaacat
     7501 tccctcacat aacataagta agaatgatat aagaaatcca atcaagctat ttttagttga
     7561 catataatta gatttattag atattattcc accaagtatg aaaaatacag gtgcaaaaaa
     7621 tataccattt cgtgtataat ctgatacttc aaaaatatac ccatataaat tctttaatag
     7681 tggtattttt tctaaaaaac cataataact atcaccaaac ataccaatta cataaataac
     7741 aatagtgatt actagtgagt actttaaacc tagttttttt acaagaaagt atgcaatact
     7801 tgcaccaata attgatgcag gaagatacca taaatgatat aaagtcccat caaatacaat
     7861 gtccttaata atattgggca ctaaattatt catggaaaaa tatccagtgt aaatatttaa
     7921 gggaatataa attacaatag ctatcccgta aataaataat acatttttta gaaaagattt
     7981 cagtttatta caattataat tatatgttga aattagaaaa aagcctgata gcataaaaaa
     8041 gaatggcaca gcaatacgag caattatact agttaacaca aagtcagcca ttttactata
     8101 tgataataat ggagatgtat gaattgcaat tataagtaat gcagctataa tcctaaaata
     8161 atcaagccct gcataagatt tattcctaat cattcttgtt cctccatttc ttagtccttt
     8221 ctatttctcc aaaatgtcac aataaaacca tccttattgt tacccgatat ttgatataca
     8281 cattgttcag gtatagagat tctcacttct tctttagtat gaataggcac ataaaagatt
     8341 tctataattt tttctttatc ttctattttt aaacaatttg tatacgaaaa tctcttaata
     8401 tattctatat attcttcaag agcgagattc attttattga taatctcaga atgcggatat
     8461 cccacataac gaaaatgcca tggttcttga gatattcctg ttatttcttc ttttttacac
     8521 tgataacgct ctataaaacc ataatgtagt gctgccttac gaaactcatt acaaatacca
     8581 ttataaggaa attcagggca gataaaatct ataacttctt tctttaaccc taaatcaata
     8641 gcaagccctg tctgatgttc actgtgattt ggaagtgcta caaacttttt agtaaagtct
     8701 tttccattgt cacgcagaga acttgaatat atatcttgtt gttcctctgc agtacgatat
     8761 ccacttacag gtactatttc atttattcca catatacttt ttaaaatatt ttgtaaaatg
     8821 tttgtagctt cacgtttcat aagaatactt ggaaaccttg tatctacagg aattaaatct
     8881 atatcttcag atgttttaac tggtaaaaaa gcatttacta gaattagatt cccatcataa
     8941 actttatctt tagtcaataa gatagtattt gtcattttat cttctttatt tttatctaat
     9001 aaaatattat tcatcatttt atgtacacct caattatctt gtccaatatt tcttcaaaag
     9061 acagtccaat acctttcatc atatttggat aacgactatg agaagtaaag ccaggaattg
     9121 tattgacttc attaaaaata atttcaccag atggagttaa aaacatatct actctagcaa
     9181 atccagaaca tccaagagtt ctataaataa tttttgctgt ctcttgtatt cttttctcac
     9241 aatccaaatc aattctagct ggcatatgaa tttttgaact tttcaatgta tatttttctg
     9301 tataatcaaa aaatcctttt gacaattcta tttcatctac tcttccaaca agtaagtcat
     9361 tgtttcctaa aactgcacac cctacctcaa aaccatcaat attttcctca attattactt
     9421 cattatcatg ttcaaaagcc aagtctactg ctacttgtaa ctcttctttt ttataaattt
     9481 tactaatacc aaatgatgaa cctgcacgca caggttttat aaataatgga taagttaagt
     9541 cttttgacag ttcagccaca ttaaatttat ctgagtattt tagtaatatt gattttggta
     9601 ctttaatacc ttctgaatga acaagtttat gtgatttgtc tttgtccata cataatgctg
     9661 aagaaagagt accacatcca ataacctgta tacctgccaa ttcaaataac ccctgcactg
     9721 ttccatcttc tccatttttt ccatgcagta caggaaacac aaaatcaagt ttagtgtatt
     9781 taatatcatt ttcacatgat tcaatagcac tatttggaat atgattctga gaaacagcat
     9841 caggaattaa aaattcaata ataccatttt tgacacggtt ctgcgaaaca ataactggaa
     9901 ttatatcagt atccctttca aaccaagtgt tattctggat attattataa tctccaatat
     9961 aatgatacca attaccctcc cttgtaattc caattggtat aatatcatat ttttccgtat
    10021 tcaaattctc aagtactgca aaagctgatt gtaatgatac atcatattct gttgagtttc
    10081 caccaaatat aactgcaatt tttttattat tcatctttga ttctcctttg ttgatttatt
    10141 tatttttata caatctacag aaacatgtta aaatactttt tctatttcaa atccaatcaa
    10201 ccttcactta caattgcatc tgttccacat aatatatttt cacatttttc tattgatata
    10261 attactgtat caaaataaaa gcactcttgt tttgatacag taattatatc aataacaaga
    10321 gtgctcttta tttatttatc ctaaagaatt tcctattttt ttatacttaa attcttacaa
    10381 atttcctaca atcggaataa taaccttaaa ggtaattata ttgttctcgc tatgagctgt
    10441 tattgtcccg ttatgaagct ccactatttc tttagctata gcaagtccca acccagctct
    10501 accactgttt gaattacgag atgtatccaa cctgtaaaat tgttcaaaaa tacgctcaag
    10561 tttttcctct ggaatcgtat tgctatgatt tataaattta agctttatat aatcatcctc
    10621 ctgtacagct gttatttcaa tagtagtatc ttcaaaacta taataaacag catttcgtaa
    10681 taagttatca aatacacgtt gcattttatt aacatcacac cttatcatca tatttggtgt
    10741 aacattcagt gtatatttta aattcttact catcaaagta ggtttaaact catatgttag
    10801 ttgttcaagc atacgagtta agcttactgt actgtattca agtgatatat ttgataaatt
    10861 aaaccttgta atttcaaaga attcattaat taaatcttca agacgctctg ccttatccaa
    10921 cgaaatagat aaatattttt ttcgcagttc ttcagaaatc tgactttcat ctcgaagtag
    10981 tgtcagatac cctataactg atgtaagtgg tgtttttaaa tcatgtgcaa gataaacaat
    11041 aagttcattc ttacgttgtt ctgccatttg tgtctcaaat ttacgttgtt caagtatatg
    11101 tttaattgag tttattttct tttctattgc caataactct ggagataaag ccacttctcc
    11161 aacatcttct ttgataagag agtcaatacc ttgatttatt tcactaaaat attttgtaaa
    11221 ccaatttaaa taaacacgga atattataaa aaacacaatc accatagcta gtatcatgaa
    11281 gagctccata ctattacgga aaatctgctg atataaattc aatgcagctt tatattccat
    11341 acgaaaagca ttttcaaaga atgatactat ccagatagaa aacttaccat acaatataaa
    11401 agaatataaa atccatatga aaataatggc tataaatgcc attaaagctg ttctcataaa
    11461 aattttattt ctcacttgag taaagtcagg attgacttta atatttttac ttttcaattg
    11521 tataacctac tccccataca gtttttataa atttcgggtt tttagaaggt tcatgaattt
    11581 tttcacgtaa tcgccctata tgagccataa ctgtgttatt attatcaaga tatttttctc
    11641 cccatacagc ttcaaacaat tcttcagaag gaaccacttt tccttgatgt tcacataaat
    11701 accaaagaat agaaaattca atcggtgtta atgctacttc tttcccaaat aaactacatt
    11761 tatgtgtttc tttattgata atcaatcctt taatatcata ctcattaaca attatcgact
    11821 gttgttcata agaattatta tatcgcatat atcttcttaa ttgtgtcttt actcttgcaa
    11881 caacttccaa agggttaaat ggctttgtta tataatcatc agcacctatg gttaaaccca
    11941 ttatcttgtc tatatcttca attttagctg ttaacataat gataggataa tagtattttt
    12001 ctcgaatttt ctggcaaata tgaaatccat caatatcagg aagcataata tctaaaatag
    12061 ccatatccaa atttttagat tcaatacatt ctagagcttc cttacctgta taaaacttgt
    12121 atactgtaaa accttcattt tttaaatata cttcaattaa atctgctatt tctttttcat
    12181 catccacaat caaaatattt tcgttcatag attatttttc actctcctta ttatcgaggt
    12241 catcatttat aatattggta aatttacata tcaaataatt acaagcatat ttgtaactaa
    12301 tttttataag aaaagaacta ctacatcaga atgtgttgat gctactattg gaacacaaaa
    12361 actgagatta attgttatta taaaaactcc cctaacacgt ccttattatc gcataacagg
    12421 tattaacttt caataaaaat atctatcagt caaacacaat tcaatttatt ataccagaga
    12481 caatccatat aaaaaacaat taaacctatg tatgtaacca cagacattac taaaatttcc
    12541 atgttatttt atataaaaat tcaaaaaagg agttgaattt aaaatttgat tagaaaaaaa
    12601 tcaattttaa gacaactcct ttttatttta atgtatttaa attatagtat atcagccttc
    12661 ataatatcct caaaatcacc tttttcagat attaatctta tcaatttaac agtacagctt
    12721 aaatcattta taagtatagc tccaactaat gaaccatctt tcataaataa ttttttatat
    12781 attctttgac cttcatcttt tgatgaaatt tctttatcaa aattttgtat attaccaata
    12841 ctaaatactt ttacattcat tgcatctaat gaaactggat atacttcact ttcaaatgtc
    12901 aaattatctc cacttgcatt tgcccctgct atttttccca tttctactga agatggccat
    12961 attgctaaac taatatttcc cacttgtgca acatctccac aagcatatat atctttaaca
    13021 tttgtctgta atgtcttatc tacaacaata cctctgtcaa tttttacaga tgtatcctga
    13081 accatttgag tgtttgctct tacaccaatt gaaaatacta ccatatcaca gtcaacacta
    13141 tctccatctt taaatacaac tttttgagct tttccatcac cttcaatacc atcaactgct
    13201 acacccaatc ttacatctat tcctgtatct cttacacatt gttctaatat tttactgcct
    13261 tctttatcaa gttgttttga tagtatgcta tccatggctt caactactac tacttctaat
    13321 ccttttagtc taaattccca tgcagcttca agtcccaata aaccgccacc aatcactaca
    13381 actttcttag atttttctag tgcttctttt acactatata aatcttttat atttctaaga
    13441 gtaaacacat tttctaaatc atgaccctta attggtggta taaagtttct acttcctgta
    13501 gctattacaa gcttatcaaa tttaatagct ccatcattca ctataatagt tttattcact
    13561 tcatctaatt tatctacatt cgttcctaat attactacta tattattttt atcataccaa
    13621 tctttatctt ccatgtagaa atctgaacca agttcttcat ttataccatc agaaagtgca
    13681 ggtctatagt aagtcaactc atcttcatta gaaatcatag ttatcttaca agttttattt
    13741 ctttttctta tagcatctgc tgcataaaaa ccagctgctc catttcctac tattacgaaa
    13801 tattcatcag tatcttttct aaaagtaact tcatcttctt caacttctat aaattggtct
    13861 tccttagctc cacatacagg gcatacacta ggagcactat caccttcaaa aacttcatta
    13921 caaattacac atttaaattt cttagttttc tttttacttc caaaagtaga atttaactta
    13981 tctaggaatt tacctgtata agaatgtaat ttatcaatct cttcatttga tggtttaaag
    14041 ttaacagcaa aaggttttac tgctgtcatt ctaagctgac ttattctctc catagcattt
    14101 tctatacctt ctccactcca tccatatgaa ccaaatactg aagcaacttt tccaccatga
    14161 agaactgggt ttaacgatat taatatatcc catataggct ttaacgcatc tcctaatatt
    14221 gttggtgttc caaataatac accttgtgat actgctattt ttgccaaaat atcttcttgc
    14281 ttatgctcta ttacatcaaa taaatttact ttatagttgc tatttttttc aatatatgct
    14341 tttatagctt ctgccataat ctttgtatat ccatgtgcag aaacataaca aattgtaact
    14401 tctttttcta actttatttg ttcatttaca ctccagttgt aataaaggtc tataattttt
    14461 ctaggatttt cagttaatac tggaccatga cctggacata cagtatctat atctaaatct
    14521 tttatctttt ctatggcagt taccatagat ggtttaaatg gtcccattat acaatcataa
    14581 tagtatctca aagcatccaa gtaatccttt tcttcatctt catcaagtgt atttactatc
    14641 ttatcattac tatagtgact accaaatgaa tcacatgtta caagagtttt atcctctttt
    14701 atatatgtgt atatggtatc tggccaatga agcataggta cagagaaaaa ttctaaagtt
    14761 ttgtccccaa ttgataatgt atctccatct gtaactgcaa catattcaaa gtctttgttt
    14821 acgatttcct taagatattt aattgctgtt tctgaagcta ctacttttgc atttttagct
    14881 aaatccaaaa gcttttcaac gcttcctgca tggtctggtt cagtatgact tacaacaata
    14941 tagtctattt tttcaaaatc tatatttaaa tcatttaatc tttcaatata attgtcaaaa
    15001 tgcttatctt taactgtctc aaataaaact gttttttctg ttccttttaa tacatatgaa
    15061 ttataagtag ttccataagg tgtatacata attatgtcaa atactcttaa atcagggtca
    15121 agcgaaccta cccaatataa attttctttt atttttagcg atttcaatga cataccgtac
    15181 tcctcctaaa atagttattt agtctcttta taccctactt gttatatttt aaacaaattt
    15241 tattcaaaaa agaaattatt ttaaagatag tttaaatctt ctaatcttct ttttctacct
    15301 tcaatacagt tcccttttta gaatctactt ttatgtcata ttcattttta tctttatcta
    15361 ttcttatttt ataaaaagta acatctaaat ctttatctaa actccactct ttaaccattc
    15421 cattttgttc ttttaatgca atttccatag cttgttgagg tgtcattatg tcattaaaat
    15481 ctaatttttc ttttctagcg actccattta aatcttctga atccaacttt tctgttttat
    15541 cttctaatac atcttttgta ttagcatcaa ttttcatctt atattcatta ttatcatcta
    15601 caccttccac agtataaaca aatttattta aatctctctc aagacttaaa tctactatat
    15661 cagcatcttt gtatttgtct ttaaatatgt ttattgcatc tgtgtatggc actttaaaat
    15721 cttgttccat taaattttca ttattttttt catcttttaa agcgttattt gatacatttg
    15781 cctccttttt tgtattatct tgtgcttgtt ctgaatcctt agttggactc ccattacatg
    15841 cagttaataa acctgctata agtataccta ccactaataa atacattttt ttgcacataa
    15901 aaaccctcct taaataatta atttattcta tattatgccc tctaaaccct ttatttaccc
    15961 ttttatatta aaattcctcc tattttagta gttttaaaac tttatactca ttaagattaa
    16021 atctacataa aaataatatt gtctcaaaat agaaacagac ctctttttta ttgttgaaat
    16081 aataaaaata tatttgattt catacaaaaa gagtgcctca tgaactaaaa agttcatttt
    16141 gagacactcc cattttttat attttcactt acaaaataac aggacttatt aatttttcac
    16201 aaatcttata aatatatcta tataatattc ttaaatttaa acactacata aaaaaattaa
    16261 attttattta attcttttac cgttaaatcc attttaggat atggaatcgt aattccttct
    16321 tcgtcaaacc taactttaac ctcttctagt aaatcaagat aaattgtcca ataatcttct
    16381 gtcttgcacc aaactctagt tgcaaattta atagcattat ctccatgttc tactactcct
    16441 ataaatggtt ctggttcatt tattattaat ttatgattat taacaatatc tttaagtact
    16501 cttctaacat gtagtatatc atcttcatac cctacactaa aagtcaaatc aactcgtcta
    16561 gtattctttg ctgaatagtt tattagacta tcatttgata cagaaccatt aggtataagt
    16621 atctgtttat tatctggagt aaccaattgt gtataaaaaa gtcctatttg ttcaaccgtt
    16681 cctccatggc ctgctgcttc tacgtaatct cctactttaa aaggtctaat caatagtata
    16741 ataaatcctc cagcaaaatt tgatagacta ccttgtaaag ccaaacctat tgctacccct
    16801 gctgatgcta tcacagcagc aaaactggac aaactcattc cccaatattc cagaacaatg
    16861 attataacgg caactttaag tacactcaaa agaagagact tcaaaaacct tcttaaagtt
    16921 aaatcaatat ctcttttact aagtaataac ataacatggt ttacaaattt ttttattatt
    16981 ttaaatccaa tagaaagtat aagtatgcct attataagtt ttactccgct tgtagtagcc
    17041 cattctaata gactttccat tatacctccc atatccatct tattaatatc taaattattt
    17101 aaatctaact tgcttatatc atctattttc gtcgtatcca taaaaaactc cttaccttat
    17161 attttagaac caatattata gtttaaagtt cttattttct atttaatata atacaatacc
    17221 atttgtacat agtaaacaag atttatattc caattataca tcttaggaaa atgcaaacta
    17281 tttgttatat atatatgcaa aatataatac taaattaaac tattaataaa acactatata
    17341 gccttaaaat catcattttt tacccctata tgagatgata ctatagttat aatacttagt
    17401 actataaata ctacaaaacc ttttttatat ccatcaacta tattatatga atttacttgt
    17461 aaagagatag ctataatcaa attaataatt ccacttccta taaattcaaa agtattaata
    17521 acactagttg ccagtccaga ataaatttct ttatttttat acttggcatc attaaaaacc
    17581 tgtagatgtg acataactat acatcccata ataaagaatg atattggtaa aactataatt
    17641 ggtggtttta cctggtatac aactacaatt acaatccata taaacaaatc tatcattgcc
    17701 ccaaatttta ttatattaaa tttgctagac cttattttag caaatacaaa atccataatt
    17761 attgaaccaa atataaatcc atatgtaaaa aatgatacta taaacgcaga aaaactctta
    17821 ctcacaccat aaacgtccat tatgtatctt actccccaca agcttataaa tgcacttgta
    17881 agacccacaa aagagaacat tatcattgaa ttataccatg ttgatttgtt ctttataaca
    17941 gacttaatac catcaacaat attaaccttc tctgactttt catagggttc agtatctata
    18001 ttgaatccat actcttttgg tgtatctcta acaataatat acataaaaca tccaattaca
    18061 acaccaatta taccgattaa taaaaatgaa tttctccaac caacaagttc agacaaaaac
    18121 acaagtggaa aagtagcaag aactccacct aaatttccta caaatgaaaa tttagctgtt
    18181 gcagaagcaa attctgactt attaaaccat ctcccttgaa tttttaaaat acaaagtaat
    18241 attactgatg ttcctgcccc tacaattaca cgagaaatat atgctaattc tatactttga
    18301 atcaaaccaa ataaaattga ccctaaactt gccattatta ttccaagact gctaattttt
    18361 cttgctccat atttatcaat caaaattcct gcaggtattt gcataaatgc atatgcatat
    18421 aggcaaaagg atgctatatt tgaaatttgt atagaattaa aacctaactc atttgctaaa
    18481 ttgtctgaaa caacagcagt tgacatcctt aaaaataata ctaaaacaaa tgaaaatgta
    18541 attatacccc aaacactcca cttagccttt gtataacttt tcatatcttc tccctcaaat
    18601 ccatatattt ttaaattttc ttgatataac ttatataaag tgtatagtta ttcttacaaa
    18661 tttttacacg caatatcatt atacaacata tgtatatatt taactagaaa taaaacacta
    18721 ttcatattat atacatgaat taactacata aataataaga gccatctttt aacattactc
    18781 aaaagatggc tccatataaa aacatttatt ctaaatttaa ttttttatct aatataaatt
    18841 ttgtagcgaa aaataacgct attgcaacta caagactacc aagtattgca agcataaaac
    18901 taggactctg ataaagatga tataccatat ttgtatcttc atttggtaag atgcttccaa
    18961 ctgcatctcc aacaattgat attactatat ttatagctat aaaagaagca aatgccacta
    19021 tgtttctatg tttattgaat tttggtaatt gtcccattga gattgatgta tataataaca
    19081 aaataaatga tgaatatgaa ataaacatta ctactactaa taataaaata tctaaaaagt
    19141 tttcagaaat ccatttaaaa ctagtgttaa acaaatctaa aatttcaggt agtaaaattc
    19201 cagaagtacc aaaaagcata agaactccaa aagtgaatac tgcaactata aaactaagta
    19261 cagcgtatat aagtgctgtt atacatttag ataatataag tgaactagtg ctaacaggaa
    19321 gagtaaacat taaataacct tcatccccca aaagattttt tctaaacctt tgaattgtaa
    19381 ctacaatagt taaaactcct aatgccataa aaagacttgt taatactagt attcctattc
    19441 cctgtacctg aagtatattt gtattcataa atattccgtt aaatacagct actatcaata
    19501 tggctatata taacggtata aatattcttc cacttgcttt taactcatat tttaataatt
    19561 tacctaacat ttaaattcct ccctaaataa tgcatctata gattttcctt tttcttctct
    19621 tattgcttca acgtctcctt gtaacactat ttctcctttt gatatgaata ttatctcatc
    19681 acatatgttt tctatttcac ttattaaatg ggtagcaatc agtaaagtac tatcttcaga
    19741 ataatttttt aatatagtct taagtatata tgaccttgca gctgggtcaa caccacctat
    19801 aggttcatcc aatatatata tgctcgcatt tcttgacatt actaaaataa gttgaacttt
    19861 ttctttagtt cctttagaca ttgtctttag cttttcattt acatcaatat ctaagctttt
    19921 aatcatctca tttgctcttc taacatcaaa atcactataa aaatcataga aaaacttaag
    19981 taaatctgat accttcatcc aatcatttaa ataagttctt tctggtaaat atgaaactat
    20041 ttttttagta tctatggatg gtttcattcc atttatcata atttcaccat tatcaggctg
    20101 taataatcca ttcattagct taatcattgt acttttgcca cttccattag gtcctaacag
    20161 cccgactatt tttccctttg gtatatttaa atcaatattt tttaaaacat tcttagttcc
    20221 ataactcttg tttacacctt taaattcaac tattccatct tgagaaacat tgcttatatt
    20281 tttatccatt ttataattct cctttcagat tttctgtaat aagttttagc atttcatctc
    20341 tatcatatcc aagcttttct aaggttactt ttaatgtatt tatctccaca ttagctattt
    20401 cctctttcat agcttttatt ttttccttat catctgttac aaatcttcct ttagttctct
    20461 gactataaac taaaccttgt ctttcaagtt ctgttagagc cttttgcatt gtatttggat
    20521 taacttccgc atcttctgct agtgctctca ctgtttctaa tttagatcct attttaattt
    20581 cccctgaaat tatttttaac tttaaatgtt ctactaattg tatatatata ggtttgttat
    20641 tatcaagttc ccattccatg ttcatgcctc cttattatat tattgtatta agttgttaat
    20701 acaataatat aataatacaa agtatatgtc aattactttt tagtaaaagt ataaaaaatt
    20761 ttacaaaaaa aatgaacact acattagagt ccatttttaa cacaatttat taaattcttt
    20821 atattacaga ttaattaccc tcttaccaag ttagatacaa ttaaatgttc aaattcttca
    20881 attggcattg gctttccaaa caaatatcct tgtgccatat cacactttac ttcttttaaa
    20941 agtttaaact gttcttctgt ctcaactcct tctgatatga ctttcatacc caactctttt
    21001 gccatttcta ttactttagt aacaattatt ttgcttcttt tactattata agtttccata
    21061 ataaattttt ggtcaagttt taagatatcc ataggaattt cttttaacat atttaatgat
    21121 gagtaccctg agccaaaatc atccatagat attaaaaatc ctattttttt taaattattc
    21181 attatatcaa ttaaaatatc aaaattatca aatatgatac tctccgttaa ttcaagttct
    21241 atatactttg caggtatatt gtatttatca attatatttt tgtatgtttc tataaaatta
    21301 ttgcaatata aatgtatcct tgaaacatta actgaaacag taactggatt tattcctgca
    21361 tcaatccatt ttctaaggta tatacatact tcttcaagca catataaatc taaatttata
    21421 acaaaaccgt ttctttcaaa aaatgggata aattcatttg gaggtattag ccctttatca
    21481 ggacgtttcc acctaacaag tgcttctgaa ccttgtatct ctcctgtatt aagttctatc
    21541 ttaggttgta agtatataat aaattcttta ttttctagac ttgaaaacat tgtattttcc
    21601 atctcttttt cttttatctc ttggtcatga atcttattat cataaaatgc atatgaactt
    21661 ttatgtcctc cctttgccat tttatgaggt atattagccc tatctatgat tgagttgata
    21721 tctctatctt ctggaagtat aaaatagatt ccacaactta aaactaactt atatttttct
    21781 tcttgattat tattaaacaa gtctaattct tcaaatatag tttttaacct ctttgttata
    21841 tcttcttctt ctatatattt cataattata ataaaattat ccgcagatat tcttgcaaac
    21901 atttcctctt caaatatatt cctcttaata atctttgtta aattacgcaa aatttcattc
    21961 ccaacttcat atccaaataa atcatttata tatttaaatt tatcaatatc tatatataat
    22021 aaagcatact ttacttgagt atttttacat agtattttgt tagcatcaat tacaaattta
    22081 tctatagtat ttgctcctgt aagattatct gtatatgcta tcctttgcaa tttaatttta
    22141 aaatttaatt tatcatatat tatatagaat ataagtatta cacatattat agccataata
    22201 agtattattt gtattgcatg tttgctaata agctgattat tagacacaat agactcaaag
    22261 ttatttatgg agttgttttt atagagtttg cataaagaat agactggtgt tgtgtatgta
    22321 atttgtaaaa ttatacaaat aaataaaaaa aacatcaatt ttttttgtaa attcctcata
    22381 aaaatttcct cccattaaaa cacctctatt ttattagcaa ataagtacat ctcaaaagtt
    22441 tccatttctt gtaaaatgga tagtttatat ctactttaat tttgtaaaat tttctgctac
    22501 tataattata gaatgtagta tattcattgt cttaactatt gatttatata tttcatatag
    22561 gtataataga aaataaattg gattattttg gcatataatt atttattaaa aggtgattat
    22621 ttttgtttaa attgtctgaa aaattttatt tttatattat aattttaata ttttcttttc
//
LOCUS       DAMKMI010000052        22458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMKMI010000052 DAMKMI010000000
VERSION     DAMKMI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.053"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(353..493)
                     /locus_tag="PC429_001862"
     CDS             complement(353..493)
                     /locus_tag="PC429_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847369.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(751..1911)
                     /locus_tag="PC429_001863"
     CDS             complement(751..1911)
                     /locus_tag="PC429_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF3847370.1"
                     /translation="MKLNKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGSVSAVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSISDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(2082..2285)
                     /locus_tag="PC429_001864"
     CDS             complement(2082..2285)
                     /locus_tag="PC429_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF3847371.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2372..3406)
                     /locus_tag="PC429_001865"
     CDS             complement(2372..3406)
                     /locus_tag="PC429_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF3847372.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTATSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKEAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSKFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(3928..5289)
                     /gene="rlmD"
                     /locus_tag="PC429_001866"
     CDS             complement(3928..5289)
                     /gene="rlmD"
                     /locus_tag="PC429_001866"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF3847373.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIIVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(5316..6038)
                     /locus_tag="PC429_001867"
     CDS             complement(5316..6038)
                     /locus_tag="PC429_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF3847374.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(6038..6367)
                     /locus_tag="PC429_001868"
     CDS             complement(6038..6367)
                     /locus_tag="PC429_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF3847375.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6697..7623
                     /gene="fba"
                     /locus_tag="PC429_001869"
     CDS             6697..7623
                     /gene="fba"
                     /locus_tag="PC429_001869"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF3847376.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(7758..9389)
                     /locus_tag="PC429_001870"
     CDS             complement(7758..9389)
                     /locus_tag="PC429_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3847377.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(9872..10909)
                     /locus_tag="PC429_001871"
     CDS             complement(9872..10909)
                     /locus_tag="PC429_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF3847378.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(10928..11713)
                     /locus_tag="PC429_001872"
     CDS             complement(10928..11713)
                     /locus_tag="PC429_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF3847379.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(11746..12879)
                     /locus_tag="PC429_001873"
     CDS             complement(11746..12879)
                     /locus_tag="PC429_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF3847380.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(12927..14054)
                     /gene="hadC"
                     /locus_tag="PC429_001874"
     CDS             complement(12927..14054)
                     /gene="hadC"
                     /locus_tag="PC429_001874"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF3847381.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(14054..15280)
                     /gene="hadB"
                     /locus_tag="PC429_001875"
     CDS             complement(14054..15280)
                     /gene="hadB"
                     /locus_tag="PC429_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF3847382.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(15295..16095)
                     /gene="hadI"
                     /locus_tag="PC429_001876"
     CDS             complement(15295..16095)
                     /gene="hadI"
                     /locus_tag="PC429_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF3847383.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(16134..17333)
                     /gene="hadA"
                     /locus_tag="PC429_001877"
     CDS             complement(16134..17333)
                     /gene="hadA"
                     /locus_tag="PC429_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF3847384.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            18010..19008
                     /locus_tag="PC429_001878"
     CDS             18010..19008
                     /locus_tag="PC429_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF3847385.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            19370..19897
                     /locus_tag="PC429_001879"
     CDS             19370..19897
                     /locus_tag="PC429_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF3847386.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            19924..20520
                     /locus_tag="PC429_001880"
     CDS             19924..20520
                     /locus_tag="PC429_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF3847387.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(20585..22168)
                     /gene="asnB"
                     /locus_tag="PC429_001881"
     CDS             complement(20585..22168)
                     /gene="asnB"
                     /locus_tag="PC429_001881"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF3847388.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         6962 a         4075 c         2677 g         8744 t
ORIGIN      
        1 agtcatcttc ttaccagttt tttatccact tttctaatga agttattttt tcatatgtta
       61 attcctcttg aaataatgat actaaaagac tttttataaa attataactt aaaagtttat
      121 gtattttttt gatatgaatt tatagtactt atcttcttta attgagattg tgtatattta
      181 actaaacatt acaacaataa ttctatgaaa tcaaatcaat tatttacata tttttttaga
      241 acaataataa gtatatattc acaaaatttt gtgttaatat ttttaatttg tgatataata
      301 aaagcaagga aacatatttt acttaacttt gagtgatgtt tccttcaaca aattattttg
      361 aagatttact tttctggaac tttatattaa gttcccagcc actcttagtt ggtcgctcag
      421 agtggctctt taatttatcg taaatgtaac tggctataac accagttata atactcatca
      481 ttagattttc catagttttc acctcctctc ataagaaagt gaagaaactt ccatggaaac
      541 accactcata gctttttcaa atatttattc cttgccatat taattatatc ataaatattg
      601 gaaacataaa attttaatac ttatcgtctt tattattatt ttttataatt aataattttt
      661 tttaataata cataatataa atttatattt cttcaataaa aaagacaatc ttaattaatt
      721 gtctttttta atatatattt ggctaataca ttattttaca caaacattac aagggtctat
      781 tttaccttgt gacttagctt gttttaatgt tactttattt attgttttac ttctttttag
      841 tgtagaacaa ttttttgtta gatgatatga ttttccatta ggagttacat atactgtact
      901 tttatcagtt aatccaggat tattagagct aggctcatta cttggtttgt tcgtattatt
      961 tgaattagta tcttttacaa tgcctgtatt tgaatcccca acattattag ttatatttaa
     1021 acattgtttt ataacaacat catcaatatt tccaatagca gttattttac ttgcattttt
     1081 taatatactc ttattacttc catttccaac taacactata ggtgtttttt gtgcaattgt
     1141 agaaccaact aaagaataaa ctaaatcagt cccacttgct atgtaaaatt cttttgagcc
     1201 attataaaat ttatttatta tttgtttatt tgtatcgtat ctatctactc cacctattct
     1261 tgttgcttta gtttcattta ttaacttatc actaatactg acttttccac caattgcata
     1321 agtttctaat ccatctgtag caaaacttgt agtgtttcca tcagttaata ctattggaga
     1381 tttatctcta actgctatag aagcaacact catagcatct ggttcacctt taaaagcatt
     1441 agttaatatt acttttttaa cagcacttac acttcctatt tcttttgcaa ctttatgact
     1501 tgtatctatt ctatcagttc cacttattct atttatgctt atttttttat cacttaatat
     1561 tttctgaact gatgctccta tcgaattttt tcctccgatt agatatactt cttttacctt
     1621 ttctaatctt tttaatgttt ctgaagggat tgtatctttc ttagttaaca atattggggc
     1681 atttttagct cctgacaatc cacttgctga caatccatca gataatccat tttcagaatt
     1741 aataagtatt gcaatatcat aattttgttt atctgctatt aaacctgctg tttcatactt
     1801 atttgtacct tttattgaat taatagaact aagagcgttt gatgttggac aactagcaat
     1861 caataatgaa acagacatcc ctattgccaa tagtgttttg tttaatttca taatgaaatc
     1921 ctcctataaa ttttataata ttacttaaat attataattc tacataaaag aatattttcc
     1981 tttatgaaat tagtttatat ttcaaaattt tgtcactttt taaatctata tactaaaaaa
     2041 tagactattt gtaacagtct atttcttagt atatcaaata tttatttagg tttacgtttc
     2101 tttgtactag tttttgtata tgatattcca gttccaggta tactcaatgt agctgatttt
     2161 cttccttttg aattaactga cactctagca cctttaacac cagcacttat acctacactc
     2221 ttcttaccta aatttaattt gagtcctcct cctagattta tgcttttcct aaagttaaat
     2281 cccatagata taaccttccc ttctatattt taaatattta tacttaaaat tatatttgtt
     2341 atacacttgc catcattggt atatctagca tctatatctt tttacgtttt ttcttcttat
     2401 atactactcc agcaactaca gctgctgttc cagctccaac cccataagta gcatttctac
     2461 ttttctcttc ttctcttttt tctaaacctt catcatatac accttttaac ttagtttcaa
     2521 tagacttcat ttcatctggt atactaaaat cttcttcatt gtaaccttga ttatatacac
     2581 tttcttttgc tttaggtaac ttaaccttac cattatcata tccttcttta aaccaattag
     2641 catatttaac attacctata gttgatgtgt caaatctttc tcctttgata gcactctcaa
     2701 atccttgagt agtgtaatca ttttttaatt gttcttgtat ttcgtcataa gcattattat
     2761 aagcatctat aaatttagaa tctatgtttt cttcaaatgt cattttttct ttatctttat
     2821 tgctatcctc tatcccttta gctttatatt cttctatctt tttagtctta tattcattgt
     2881 atccagttga atatccggta tcataagcat tttttaaaac ttctttttca tatgtattat
     2941 tagatttagc acctgtaagc cctagattat atccagtctc tttagcctcc ttttcttcct
     3001 cttctaattt agccttacct ttttcatatc cttctgagta ctttgattca tatgtatcac
     3061 tataatctcc actataattt gatgaactat aatttccttt atacccatct tcataccctt
     3121 gattatatcc tttttctcca acagacctct tttgcttttc ttcattatta accgaagttg
     3181 ctgttccaga actgcttcct cctccagtat aaggacaacc accattatta tgtaaatgag
     3241 ctggataacc accacaatga tagtgataac ttcctaatcc agatttattt ttattatcac
     3301 gatgacctcc tgatgaatca gttcttcctg aatgagcata cgaaaagctc cctacactaa
     3361 tcatagttat actcattgca acagctatta actttttctt aaacataatc tatttccccc
     3421 taaaatattc tatgatttta ttatatcagt caatatatta tgtttaaacc gttaaaattc
     3481 gacaatgtaa atatttgatg aattgtattt gtaaataaat cttttagcat atcttgaata
     3541 tctgtagttg ttttgacatc atattcttca ataagttttt taatatttcg tttttgttaa
     3601 acataaaaag cgtgcacttt tctttgtata gtgatatttg atattttaat tatcactact
     3661 ttagaagaat acacacttta ttttaaaaac cctgatacta tgttattaga aaatggtact
     3721 aatataaaag atatccagca tatactcgga catgctaaaa cttcaacaac tatggataca
     3781 tatttacatg taactaacaa catgagaaac cagacggtaa atatttttga aatcattgta
     3841 gaataaattt gccacccaaa aataattatg gtggcaaata ggtggcaaat ctacttttaa
     3901 gacacactat aaaagtctga aaccctgtta ttctctaata agtttaacta cagtctcaca
     3961 atgtggcgta tttgggaaca tatccataca cttaaccatc ttaatctcat aaccataccc
     4021 cttaaaatca accaaatcaa caaccaaaga cttaggatta caagaaatat aaacaatctc
     4081 cttagcacca aatccacaaa tatccctaat agcatcctta tgaatgcctg gtctaggagg
     4141 gtcaacaata atcaaatcag gcttgtcctt taaatcctta acaaccttag ccacatcccc
     4201 agcaataaac tcacaattag taagtccatt caacttagca ttctcattag cagcaacaac
     4261 agcttcctca actatctcaa taccatacac tttcttagca gcaccagcca taacctgtcc
     4321 aatagaccca gtaccactat acaagtcaaa tacaacttta tcattgtaat ctccaataaa
     4381 atccctagca atactataaa gaacctcagc tcccttagta ttggtttgga aaaacgaaaa
     4441 tggtgaaatc ttaaacttaa gacctaaaat ttcttcttgt atataatctc ttccatacaa
     4501 tactcttaac tcatcacatt ggactgcatc tgctaaacca tcattaatag tatgaagtac
     4561 acctactaac tcagctttta aatcaatccc taaaagcata tctttaaact catacatatc
     4621 aaaatcttct tgagaagaag ttactatatt aactataatc tcattagtgt gaatcccttt
     4681 tctaactaca agatgtctca aatatccttt gtgattcatt gttctataat aaggaagctt
     4741 tttttcattg aaaaactcta cactagaaac taatatcttt gagaagtctt catcaaccaa
     4801 catacactcc tcaacagttt gtatatctat atgctttcct tttttatgaa gacctaatgt
     4861 caaaggtcca ttttttactt catctccaaa agtatattcc atcttatttc tataatattt
     4921 gttttctgga cttccttcta tccctaaaaa ctgaaatcca aataaatctt gctttaaaaa
     4981 taaatccata acttgttttt cttttatttc taattgtttt tcatatggca cagacaatag
     5041 tgtacatcct ccacactctc taaagtgctt gcaagtttct tcagtttcaa gtggtgagtt
     5101 ctctaataat tctatcatct taacttcagc tttcttactt ctaacttttt taactccagc
     5161 tctcaccttt tgaccactta ttccaccctt catatggata actctatctc caacttggct
     5221 taaagatgta ccaccaaatt ccattttatc tacttcaaat tctataacat cttttttctt
     5281 cacagacatt cctccaattt actatagtat ctttattatc tttcttcaat caattttact
     5341 tcttcatctg ttaactctct atactcacct aactctaaac tttcatccaa ttttaaattg
     5401 cccatagaca atctttttaa gtatactact tttttgccta cgctttcaaa cattcttttt
     5461 acttggtgaa acttaccttc atgtattgta agctctattt ctgattcatc atcacttttt
     5521 aatatattta actgtgatgg catagtttta tatccgtcat caagcactac accttctaaa
     5581 aaagcttcta catcttcttc agtaactact ccatcaattt ttgcataata agtcttaggc
     5641 acatgcttct taggagataa aactctatga gaaagctttc catcattagt taaaactaat
     5701 aatccctctg tgtctttatc taaccttcca acaggaaatg gttcaaaagc taagtatgat
     5761 aaatcaatta aatctaatac tgttgggtca tatttatctg tagttgctga tatatatcca
     5821 tcaggtttat tcatcatcaa atatatatat tctctatata taacttcttc tccattaaat
     5881 aatatctcat cattttcagt gtctacttga gttcctggat ttgaaacttc acttccattt
     5941 actactacag acccttgttt acaatacttc tttatttcac ttctgcttcc ataacctaaa
     6001 tttgaaagaa ttttatctat acgttgcttt ttagccatta aaaatgctct ccattctcat
     6061 aatatttttc tttttcaaat actgtaaatg ctaaatccat actttctaca tctaaattat
     6121 ttcttatact atcagttatt attcttgcta ctatatcttg tacttcttgt cctctatcaa
     6181 accaagctac ttcaacaaaa ggatatacat ctgctatttt accatctctt attgcaactg
     6241 actttataca ttctatttca aagtagtctc ttggacattt tacagcttca actaaatcat
     6301 ttatcatttt ttcacttatt ttacatatat cattttcatt aattcctctt atttttatct
     6361 gtggcatagt ttatctcctt tattttaaat ttttagtgaa ttatcatcca cttaattccc
     6421 ttcgataacg tttgaagtgg taacttcttg gtcaagacaa ctattgttat cagtatctcc
     6481 aaactaataa ggtagttcct acccttatag ttagcattat atagctaatt ttcctaaatt
     6541 gtttatattc tttattattt tatttaatcc tattgtcttc tatttaacat tatacttgta
     6601 atttatagtt attacaattt actatatact ttttttgatt taacgttata atataagtga
     6661 tgggtaaatt ttaaaagtta ataggaggag tttaaaatgg cattaattac tacaaaagaa
     6721 atgtttaaaa aagcttacga aggtggattt gctataggtg catttaacat aagtgactta
     6781 gaacaattac aaggtgtttt aaaagctgct aaagctaaaa attcttatgt tatgatacaa
     6841 gcttctatgt cagctgttaa atatgctgga cctcatacat tagttgaaat ggttaaggct
     6901 gcttctgatg aaataggtgt agatgtagct cttcacttag accatggtcc aaatatggat
     6961 gctattaaaa cttgtattga tgctggtttc tcttcagtta tgatagatgg ttctcatttt
     7021 gactttgaag aaaatgttag aataactaaa gaagctgttg aatatgcaca ctctaaaggt
     7081 gttgttgttg aagcagaact tggtgttcta gctggaacag aagatgatgt tacttctgat
     7141 gttcacaagt atactcaacc agctgaggca gttgagttcg ttgaaagaac aggtgttgat
     7201 tcattagcaa tagctattgg aacttctcat ggtgctttca aattcaaagg agaagctaaa
     7261 ttaagattcg atatactaga agaaatccaa agcaaattac caggtttccc aatagttctt
     7321 catggtgcat ctgctgttga ccaaaacgct gttgctacat gtaatgaatt tggtggaaat
     7381 atagctggtg ctaaaggtgt acctgtagat atgttaagaa aagcatcttc tatggctgtt
     7441 tgtaaaataa acatggatac tgacttaaga ctagctatga ctgctgctat cagaaaattc
     7501 ttagctgaaa atccaaaaga atttgaccca agaaaataca ttggtgctgg tagagatgct
     7561 atacaagctg tagttgaaag caagatagat gatgttttag gttctgctaa ctctataaac
     7621 taatataact tttaaatatt tataaaagaa aagagatgac ttaaagtaat taaatttatt
     7681 tttaagccat ctcttttaag ttgtataaat tatgtatctt ataatatttt aattatattg
     7741 tctttttaaa ctgacagcta aacgtgaata tgtccactct ttatctttct atggatggta
     7801 gaaggatata tacctataac ttcagcagcc tttgttattg aaccatactt atttatagcc
     7861 ttacttatca ttatttggtc cactatttta tatgcatcat taagattcat aattccattt
     7921 accactattg gtgataaatc atctgtttca ttatcaatca tatctaaatt tatcagcatg
     7981 ttaaattcat cttctccaat tacattttta gctgataata ctacaaatct ttcaatgatg
     8041 ttttttaatt ctcttatgtt tcctggccaa gaatattctt ctaataactc aataacttta
     8101 ggcgaaactt ctacttctct attgtacttt tcattgtaaa gttttaagaa ataatttact
     8161 aatggtacta tatcctcttt tctttctcta agaggaggta tttttactgg aataacactt
     8221 aatctatagt acaaatcttg tctaaattta aggttatttc taaggtcttc atgagaaata
     8281 ttagtagcac ttatatatct aacatctatt tttataggct tactaccacc tattctagta
     8341 atagtatttt cttgtataac cctaagtaat tttttttgca tctgcattgg taactcacct
     8401 atctcatcca agaaaatagt tccaccattc gcctcttcaa aaagtccctt tttacctttt
     8461 ttcttagctc cagtaaaaga accttcttca tatccaaaaa actctgactc taataattca
     8521 tttggtatag ctccacagtt tatagctata aagggtttgt ctctccttga actatactta
     8581 tatattgttc ttgctattat ttcttttcct acacctgatt ctccatttat aaatatagaa
     8641 gaatctgagg ctgcagctct cttaacaact ttttttatat tgtccataat cttactttga
     8701 cttatatact ctgtgtcttc tgatatatca ctatatttta tctgagattc aagcttaata
     8761 acccttgaaa tatctctccc tgttagtact cctcctgtaa gctttccctt agaatcccat
     8821 cttggaactg ccgtatatgc aataagcttc ccatctgggt aaacgatatt tctataaatc
     8881 tttttcttca ctttaagcat gtttaatatt attggttctt caactaatcc ttgtctcaca
     8941 aggtctgtta tatttctacc aagtacctca gttcttttaa aaggtaatat ctcatcactg
     9001 acagtgttca tcttttcaat taccccatct ttgttaaaca caacaacaac ttcatctata
     9061 tgctctatta tatcttcaat tactttatca gatattagag acttctcaaa taaataaact
     9121 atattatttt cattattatt atctttcata ttgtatatat gtgcagtcat ccttaactct
     9181 ctgaaagcta ttttcttttt actatctgtc agtatttctg ttttgtcaaa atttgaatct
     9241 aattcattta tatttctagg cttttttata tctagaaaac taaaaatact atctattaga
     9301 tgattacagt atattatttc gcctgattct ttacataaaa tggctggatt ttctacatgt
     9361 tctataagcc ctattaaatt attatccatt aatattcgca cctttcatta agtaatttat
     9421 tgaactacat acaaaattta ttgtttgctt acaataaata ccatcagcac atataatcaa
     9481 ctccttataa tattcgtctt ttcaagtagt ttatttcatt aaaacgtaac taagatataa
     9541 ttttaatgat tatagtttgt taaaaatttc attttgcttt taattgtatt ttatcacata
     9601 tttagtaatt atttgtctat ttaatacaat ttaattaaat ttaagattta tttaaaaaaa
     9661 caaagataat ccttacagaa tataactcac aatttgtaag caatatacat ataaggatta
     9721 tccagattat cttgtattat aaaattttat tcaaacagac tttttaataa ctgacaaata
     9781 cacactagca ataacttatc aaagggttaa taattatgtt actaactaat aagacttatt
     9841 caatcagtaa tatttatata gtttgtatct attaagcttt tatagcttct aataaatcat
     9901 caacgctatc taatttttct ataagcattg gaactatttc atgtaagtct ccaactactc
     9961 catagtcagc gatttctaaa attggagctg ctgggttttt gtttatagca attatgaaat
    10021 ctgatgattg catacctgct aaatgttgta ttgctcctga tataccacaa gctatatata
    10081 ggtttggttt aacagtagtt cctgtttgac ctacttggtg agaatggtct atccatccag
    10141 catcaacagc cgcacgagaa gaaccaacta ctccacctaa tttgtcagct aatttcttaa
    10201 gcatttcaaa tccttctggt ccacctaatc ctaaaccacc tgatactata acatttgcat
    10261 ctgtaagaga tactaaatct ttcttagttt taactgtttc taaaacagta gttcttatat
    10321 catcttgagt tattttgtag tctaatgcta taacttcacc agttcttgtt tcgtcttttt
    10381 cagctttatc cataacacca ggtctaactg tagacatttg aggtctatgg tttggacaaa
    10441 tgattgtagc cattatgttt ccaccaaatg ctggacgagt ttgttttatt ttcttatctt
    10501 ctgggtctat ttctaactta gtacagtcag ctgttaatcc agttccaact cttgaagcta
    10561 ttctaggtgc taagtctcta cctatatgag ttgctccgaa aagcattatt tctggtttta
    10621 tttcatctat tgcatctttt attacttttg tatatgcatc agttgtatat ttttctaaaa
    10681 gagcatcatc tgcaacataa actttgtcag ctccaaaagt gattaattct ttagctaatc
    10741 catcaacatc ttttcctaat aatattgcac aaagttctgc atctacttct ttagctaatt
    10801 ttcttccttc tcctaataat tctataacta ctggagctat ttttccttct ctttgttctg
    10861 caaatatcca tacgttttta taagaactta aatcttttat atcattcata gtttgtactc
    10921 ctccaactta tataatgtgc ttttgtttta gaccgattat tattttacta gctatttcat
    10981 cagctttacc ttctaagatt tctccttttc ctcttggttc aggagtaaat gatctaaata
    11041 ctttagttgg tgatgctttt aaaccaactt cttctttatt tacatctaaa tcatcaattg
    11101 tccaaacttt tacatcatgt ttatatgctt ttactatttt atcaacagac atgtatctag
    11161 gtgtatttaa ctcttttact gctgttaaaa gtacaggtgt acttactttt attaattcgt
    11221 atccatcttc taattgtctt tgaactatta tatcttttcc ttctattttg aaatcttgta
    11281 catatgtaac ttgaggaatg tctaatttct ctgcaatttg tggtccaact tgtgcagtat
    11341 ctccatctat tgcttgtctt cctgcaaata ttatgtcata gtcacctact ttagatattc
    11401 ccgctgcaat agtatttgat gtagcccaag tatcagctcc tccaaatgct ctatcactta
    11461 ataatattgc atcatcagca cccatagcta aacactctct tagcataaag tctgcttgag
    11521 gaggtcccat tgttataact gttatagtta catcatcatg ctcatcttta atttttaaag
    11581 cttcctctaa tgcatttgca tcatctgggt ttaatatact tggaactcca tctcttataa
    11641 gagtacctgt ttctttattt attcttactt cgttagtatc tggaacttgt tttacgcaaa
    11701 ctaatatttt caaagtctat acctccaatt tcattagatt atttattatt ttaatacgtt
    11761 tgatgaaatt accatttttt gaacttctga tgttccttcg tatattgata cgattctagc
    11821 atctctgtac attctttcga tttcataatc ttttataaat ccatatccac cgtgaagttg
    11881 taatgcttta taagcaactt catttgcaat ttctgatgca tagtatttag ccatagcaga
    11941 ttcttgagca actctcattc ctgcatctct cttttgtgct gcatcataaa ctaatcctct
    12001 agctgcacaa acttttgttt ccatgtcagc tatagtaaat tgagtatttt ggaatttagc
    12061 tataggctta ccaaattgaa ctctttgttt tgtatattta acagcttcat ctaaagcacc
    12121 ttgagctatt cctagagcta aggcagcaac acctattcta ccaacttcta gagtacccat
    12181 tgctatctta aatccttgac cttcttttcc aagtaagttt tcttttggaa ctttaacgtt
    12241 ttcaaatatt aagtcagaag tttcagttcc tcttataccc atcttatctt catgagctcc
    12301 tgttgagaaa ccatcccatt tactttctac tataaacgct gatgttcctc taagtccttt
    12361 acttctatct gtaactgcta ttactatagc tacatcacag aatggtccat tagttatgaa
    12421 agtttttcta ccatttaata tatagtggtc tccaactaat tctgctgttg tttgttgtcc
    12481 acctgcatca gaaccagctc ctggctcagt taaaccaaat gctcctatta attcacctgt
    12541 tgctatacct tttaagtatt tttgtttttg ctcttcagta ccattgtaaa gtattggtaa
    12601 tgaagcaaga gaagaaccag ctgtaacaaa agtagctgtt gaagcacatc ttctagctat
    12661 ttcttccatt actatagcgt aagaaacata tcctgctccc ccaccaccat attcttctgg
    12721 gatattactg cttataaatt ttgcatcagc taattttttg attaattcct taggcattac
    12781 acctgtttta tccatttctg ctggtaaagt atctaattct ttactaacaa aatctcttac
    12841 tgcttttctt aaaagttctt gttctttgtt atataacata gcttcctcca attttcatat
    12901 tttatagtaa atttatttta atgaacttac gctaaactca tcatctcagc aaaagtttga
    12961 atacgagttc tggcttgttc attattctga gtttgttggt cgatttcaac atataaagta
    13021 ggtattccac tatcttctat atcttttcta actaaaggat aatcgtattc ttctgggtca
    13081 cagaatttca tcatacagaa gataacacca tctatatctt tctttttaac ttcatctact
    13141 ataagtgacc cacgtttttt cttagggtca taagctaatg aacatccttc tatgtttgac
    13201 cattgtcttg ctaatctctc taacgcatca tcacctgctg gtacatctgt tctgaattgt
    13261 cttgtttctt gtgctaagtc gtcagctaca actgatatat tgttgtcttc taaaatgtct
    13321 aatatatctt tagaatcagc taatatacct gttaataaaa ctttctttcc agaacagact
    13381 tcttctggca tagcatttaa ttttgctatt aaatctttca ctagctcagt atgttcttct
    13441 tttctcataa agaacccact cttaattact aagcttctta ttgatggttt tatagtatta
    13501 gaatgtttat aagctacttc aacaaagtct ctcatagttt ttctatgttc attgtaaact
    13561 tctatacttt catgaatttt tgcttcttct atttcatatc cacaaatttc ttcaagttct
    13621 ctttttacac ctttgtactc actgattaag tattttacac cagcttcaag ttttctattt
    13681 tgtgggtgta ctaatgatat atattttata tgaggtactg ctgatttcca gttttgtcct
    13741 aaacaaatta gtgtatcaca catacctggt ataataactc cagataattc atcataagca
    13801 ccttttaatc catattctaa acatgattgc attattgaac atgcaaatgc agggaaatat
    13861 tgtttagcta aatctaattc tgtttgtcct ccccatatac caactggaag cattccagct
    13921 gcatgtataa tttcttctgg gcaataaact gggaaacaac ctatagcttt tttaccagtt
    13981 tcacttttat atttttttac agccgcattt ggattttcaa ctacttcttt cattttagat
    14041 aaaatagctt ccattatatc tcacctctat taagtttttt tctttcttcc attacttcaa
    14101 ctaaaccttg aattcttgtt tcaaattgag cattagtgaa agctctaggg tctgcttggt
    14161 caccatcaaa tccagcatat ggtaatccag tttcttcagc tgctcttctt tgcatttcat
    14221 attgtattaa actcataagt ttacagcttc tgttcatatg atagaatgct ccatcacatt
    14281 taccctctac taaagaatca actctatatt ttgtcatacg gtctaggttt acattgttaa
    14341 acatagtact atatgctact gccattccat ctaaatcatt aacttcatat tgtaatgccc
    14401 aagcatgtgg gtaaacacta cctgtcatgt taactccaaa tttagctaat gttttcatct
    14461 tgtatcctat atatggccaa caaggtatac cttccatcat tattctgtat ttttcttccc
    14521 ctctgaaaga agatttacca gttttcatgt tgtcctctaa ttcttctata agtaacttaa
    14581 atgcttctgt agtttctttt ttacctctag cacaaactat tacagccatg taagtaaata
    14641 agtcaaatcc attcattgga gaaggagaag aatctgctgg taaactcata gaatacttcc
    14701 atagtcttcc gttttcagct gatattttca ttacttcttc aaacttctta gggtcaaatt
    14761 tctttcctga tataatttct agttgtttta tagcttcttc aaattgagct ttaatataat
    14821 ctattcttga ttgagtaact tcgtcttcat tattgaaagt tgtatcaatc attattaaag
    14881 gtatatctaa ttctcttgaa atattttcat accattttat aacttggtta catatattgt
    14941 tacagcaaag taggaaatct ggagccggca tatccaaagc ttcacatcca ccattttcta
    15001 aaagaccaaa atttgttctt gcatatgcac ataggtcaat agaatatcct ttagattcag
    15061 ctatttcaca taattctaaa gaaccttttt tagctgctac tccagctgct tggttttctg
    15121 gatataatac gtttaagtca aatacttctg ctaactcttg aggaaatact gatgttgacc
    15181 aacctacagg tcttccttct tctttagctt taaatgcatt tgcatattgt tcagctaata
    15241 aatcattaat tactactcta gcttcttttt tttcagacat tttcatccct ctttttatat
    15301 atttttcact tctttttgtg attcttttgc ttcatcaaaa gcatataacg ctgctcctaa
    15361 agcaccagtt aattgaggta tatcaggtac aataatttct gtgttgattt ctcttgccat
    15421 agctcttaca ataccactat ttctagcaac tccaccaacc ataactacat ttctttgtac
    15481 tcctattctt tttactaggc tagaaactct ctttgctact gaagtatgaa tacctgccac
    15541 tatatcttca atttttgcat tttcagataa atgtgatata acctcagact ctgcaaatac
    15601 tgtacatgta ctgcttattg atacttcatt ttgagaattc atagatatac ttccgagttc
    15661 agatacatca acctctatta tttttgccat tacatctaaa aatcttcctg tacctgcagc
    15721 acatttgtca ttcataagaa agtttaatag tcttccatta ttatctaatt ttaatacctt
    15781 tgcatcttga ccacctatgt caataatggt tcttgtctct ggaattatga aatttacccc
    15841 tctagcatga cagcttaatt cacttatttg cttatcagca tctgaatagt tcattcttcc
    15901 atatcctgta actacaactt ttttaatatc ttctcttgca agacctgtct tgccatataa
    15961 tttttctaaa actcttgatg gtccagtagt cccagtacca gaggatattg tttcagaagc
    16021 tacaatatct tccccattct ttaagattac tccctttgat gcagttgaac ctatatctaa
    16081 tcccattgtg tacatcttta gcattctcct tttaaaaaat ctagaattac taattaatat
    16141 cttacaactt tactatcttt aaagttattt attttctctt catcgtagcc taaagatttt
    16201 aatacttcta cagtatgttg acctactttt ggtgctggtg tatattcttt aattccttca
    16261 tttctaaaca taactggagt attaactaag acacctgtat ttccgctatc gtatgttttc
    16321 ttaaataaga agtcatttgc ccaagcttgt tcgtcatcta ataaatcttc acagctttga
    16381 attttttcaa atggtaagtc tgcttcttct aataaagctg accactcgtc taatgttttt
    16441 tctaacatag cttctccaac tatcttaact aaatcttcaa catgattaac cattgaatct
    16501 atgttattat atctatcgtc ttctaatata tattctctat ttataacctt acagaatttg
    16561 cctaaccact tgttgtattg tattaaagct aattgaatcc atcttccatc tttacattta
    16621 tatgtagtca ttaatgggct gtttggattt tctcttgata aaggcatttc atttccgtat
    16681 tgtgctgttg ttatcattgt tcccattcca tatatagctg tatggaaaag acttactgtt
    16741 actctctcac ctttaccagt ttgagctttt ttatgtaatg ctgctaaact tcctgctgct
    16801 agtgctagac ctgcatagtg gtcaccaaat cctgctgctg tatttgctgg agatgttcct
    16861 ttttccataa cagattggct aactcctcct cttgcgaagt atgcagtata gtcaaatcct
    16921 ggtttatctt ttaaaggtcc tttttcacca tatcctaata tttgagagaa tattaatcct
    16981 ggatacttat cttttatttg gtcataagct atacccattt tttctaatgc ttgaactcta
    17041 acattagtta caaatatgtc tgcttctgat aataatttat gtaatatttc tactccttct
    17101 tttgatttta cattaatact tacacccttt ttatttccat tttctaattc aaacataggg
    17161 ttttcatcat ctgatgctgg agatttaaat gttccaccca taactcttat tccatcacct
    17221 tctataggtt caatcttaat aacctctgca ccccagtcac ctaacatttt tgcacaacat
    17281 ggtgctgcga tgaaacttga aagttctact actttaactc cttctaaaag cataacgttt
    17341 tcctccttaa tagtatcaat tataaaattt cgattaaatc actcataaaa tacttttttc
    17401 tgatattaac tgtctgatgt caaagttcct aatcttatct aaaataactt aaaaattatt
    17461 aaaattattt tttattgttt tattgtctta cacctatatt gttaagcatt tatcgtgcca
    17521 actttatatt taatcaaaat atcgcttgat ttgaatgttt tttaacaata tgcaattgaa
    17581 taatcttttt catatcgtgg catcttgcca ctatccattg cattttgcta ttctttctat
    17641 gcttataaaa gtttttttta tatatttttt tatgaaaaag cttgacattt caaaaaaagt
    17701 tttcattgct aaactcaaaa acacatgtta tcattgtaat taatcagcaa gttagttaac
    17761 taatttcaaa cttagataat tttttaataa tatttaatct aaaaaataac aatttctttg
    17821 ataaatttta gagtaattat tacattaaat aaaaaattac taaatatcaa aataacacag
    17881 ggaaaacaat ttcaaaacta aatttttaac cttaaaaatt ttgtataaaa aatataacct
    17941 tatatgctac atattatttc taaatgaaaa attaccataa taatttaata attacaggag
    18001 ggtattgata tgaaaatact agtatttgga gcacgcgatt atgaagaacc agtaataaaa
    18061 aaatggtctg aagaacataa ggatgttcaa gtggatattt atcctgaaaa catgactgaa
    18121 gaaaatgtag ttaaagctaa agggtatgat ggtatatcta tacaacaaac taactatata
    18181 gataatcctt atatttatga aactttaaaa gatgctgggg ttaaagttat agcttcaaga
    18241 actgcagggg ttgacatgat acattttgat ttagttaatg aaaatggact tatcgttaca
    18301 aacgttcctt cttattcacc taatgcaata gctgaattag ctgttactca agctatgaac
    18361 cttttaagaa agactcctct agtaaagaaa aaagtctgtg aaggtgatta ccgttggata
    18421 gctgaacttc ttggaacaga agttagatct attacagttg gtgttatagg tacaggaaaa
    18481 ataggtgcta cttctgcaaa attattcaaa ggcctaggag ctaatgtaat tgcatttgac
    18541 caatatccaa atagtgattt aaatgatata ttaacttaca aagattcttt agaagacctt
    18601 ctaaaagaag ctgaccttat aacattacat actcctttac ttgaaggaac aaaacatatg
    18661 ataaataaag atactctagc tataatgaag gatggagctt acatagtaaa tactggccgt
    18721 ggtggtttaa ttaatacagg ggatttaata gaagcactag agtcaggaaa aattagagct
    18781 gctgcccttg atacatttga aactgaagga ttgttcttaa acaaaaaaat gaatcctgga
    18841 gaattaactg acccagaaat aaataaactt ctttctatgg aacaagttat attcactcat
    18901 caccttggtt tcttcactag tacagcgatt gaaaatatag tttattctag tttaagtagt
    18961 gctgtagaag ttataaaaac aggaactgct actaatagag taaattagta ttttataaat
    19021 atattttttc attaattagc tattatctaa caaaatagcc tttaatttcc caaatataag
    19081 agagtcagct cagtatatat tgagtagact ctcttatatt attttttata ttaaacttgt
    19141 actcttctta ttcataccaa attaaattta tattaactta ctctaactac tcctactcta
    19201 tacattttac ctatttttac ttttctaatt atatacattt gaatgggtac aatctgtatt
    19261 ttacaaaaaa aacacctaac aaactatgaa aattgtatga ttaatattaa tatatttata
    19321 agaaaacata acaattatgt tagctctgga tttttaggga gggagtatat tggaaaaaat
    19381 gaataaagtt acttctaaaa aaactaccta tattgttact tcagcattat ttgcatccat
    19441 aatatgcctg acaatagcat atatattaca tattccagtt ggtggaaaca atggatatgt
    19501 tcatatagga gatgctttta tttatctagc tgcaactatt ttgccaacaa actatgctat
    19561 agctgcttct gcaattggtg ctggtctagc tgatttatct acaggtgctg ctatctgggt
    19621 aataccaact ataatcataa aaccaatact tgtattattt ttcacttcta aaagtgacaa
    19681 gataataaat aagagaaata tagttgcttc ggttgtagca ggtatagtag gattagtttt
    19741 atacatgttt gctgaaggta taattattgg aagttttaca agtgcatttg taatgagctt
    19801 acttggctta ttacagccaa taggaagttt tattgttttt ataatcttag gtatggcact
    19861 tgataaactt gattttaaaa aacgttattt taattaagaa tttaaattta tggagggaaa
    19921 attatgaata tcttatatac aattgaaaat agtatctatg taaatataac taatacatgt
    19981 ccatgtagtt gtgttttttg tataagaaac gaaaaagatg aagttgcaaa tagtggtagt
    20041 ttatggttgg agcatgagcc aagtgtagat gaagtaaaag aagccttcaa taaatacaat
    20101 ttagacgact atgatgaaat cgtattttgt ggatatggcg aaccattaat gagaataaat
    20161 gaattgatag aggtagcaaa gtttataaaa gaaaaaagta gcataaagat aagaataaat
    20221 acaaatggtc taagtgattt aatacataac aaaaaaacag ctattctgtt aaaaaatgtt
    20281 atagatgcag tatcaataag tcttaatgcc ccaaataaag aagcttataa tagagttact
    20341 caaccaaagt ttggagaaaa atcatttgat tatatgttag attttgcaag agattgcaaa
    20401 aaatatataa aagaagtagc tttttcagta gtagatgaaa tatctccaga agaaatagaa
    20461 gaatctaaac aactggcaaa aaaacttgat ataccattaa gggttagaca taagaattag
    20521 caagtaaaat tataaaacat taagaatcac ctgaatggtc tttcaggtga ttcttcctct
    20581 tgtattaaca ttttacaaaa tagatttcat accacactag gaatgtgtaa atcatccaaa
    20641 tctttctcca attatcagag tttccactaa tgtagtcatt aaaaatctca ttaatctcat
    20701 ctatgttaaa gaatttatct gctatctcac tattgaattt atctcggata tccttattat
    20761 attggtcatc agccatccat atacgaattg gcacaataaa tcctaattta tttctaaaag
    20821 caatctcttc tggtaatgtt tttgatgcag ctgtacgaaa ggcaatttta ttctcttttc
    20881 ctttcacttt atattctgat ggtattctgg atgcaatatc aaatattctt gtatctgtaa
    20941 gaggcattct gacttccaat cctactgcag tactcatttt atctatatta aaataaatat
    21001 ctccttccaa ccatatttga atatctacat ttgacatgtt agtaagtgta tctagttctt
    21061 ctgtttcttc ataaattcct tttaccactt caataggaag cacatcagga ttgtatttct
    21121 taagaatttt tcttttctca ccctcattca taatgtttgt atttccaata taaaatgtct
    21181 tcaaattctc tccataacac tctgccttat ggtatatatt atatccacca aaaaactcat
    21241 ctgctccttc tccagagtaa cagatatttg tgttttcagc tgctgccttg catccaatag
    21301 caaaaacgat agcagatgca tctccagttg gttgctccat atggtacatt acataaggta
    21361 ctgacgcaaa atactcttca ggtgtaatta tatatctcga atttttacac ccaagaatat
    21421 tagctgtctg ttgtgctaat tttgattcat caaaacgctc ttcctcataa ccacatgaac
    21481 ctgttttttc tatatctgac attgccaata tataagatga atcaacacct cctgatagga
    21541 ataactctgc agtttcattc tctgatttta cctctggcat aattttctga agtgttgtat
    21601 gaatttcatc cgtccattcc tcaatagact tgctgtggtc aggatgaaat tcaggtttcc
    21661 aataacgacc aatacatact gttttattct gatagacaag gtaatgtcct ggcataagct
    21721 tctttactcc acggaagaat gtattttcac ctgctacata tgtgaaagac atataaatct
    21781 gtaacatgtc ttcatttaac tctttcacaa atcctggttg ctccataatc ttacgaatag
    21841 atgaaccata gagaagcttc ccattttcag tctcataata atagaaaggt tttaccccaa
    21901 actggtctct taggcagaag agtttctttt ccacagtatc ccataatgca aaagcaaaca
    21961 ttccatgcat gtgatttgcc atatcatatc cccatgtctt atatgcttcc actaaaattg
    22021 ctcgttcacg ctcatctcta cataatgaag aatcgattcc aagttcttca caaagaactt
    22081 tccagtttcg aatatgccca ttgtattcta gaatttttat catatatgtg tcttttcgtg
    22141 ttaaatactt ttcttctaaa tttatcatat gtgtatcctt tcgtattaaa acatttaact
    22201 tctaaattta ttaaatgttt atatatattt attcacttat aatcactgaa aaatcgactt
    22261 ttttcaaagt aaattctttt tgaattagta ctaatctgct ctttattaat ggcataaata
    22321 tgattatcaa gtattatcag tgttataaca ttgaattttt tattcttcat aaatattgcc
    22381 ccctttatat taccaactaa atataatata tcttatgtta cttacatatt ctttaagaaa
    22441 ttctgaagat tttataaa
//
LOCUS       DAMKMI010000053        22329 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMKMI010000053 DAMKMI010000000
VERSION     DAMKMI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.054"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            142..477
                     /locus_tag="PC429_001882"
     CDS             142..477
                     /locus_tag="PC429_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847389.1"
                     /translation="MIKLWKKEDLNLLLEYPKEVVENVDNVINILDENYGCNRKLTDD
                     GGYVCIIEDIKEVENLKSNILKGLVEEFSDVIYEDEVNTYNSTLYLLSSDYSVTVISK
                     NEETEYLLK"
     gene            541..999
                     /locus_tag="PC429_001883"
                     /pseudo
     CDS             541..999
                     /locus_tag="PC429_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            1139..1432
                     /locus_tag="PC429_001884"
     CDS             1139..1432
                     /locus_tag="PC429_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847390.1"
                     /translation="MFKKENRYMTKEIAENLPIEIAILLWNLIDDLTTEKDYLQLFEI
                     NPIGLGVVEIVHKQEVPEYEARIYIQNDKIKDKLKIYVIDNVEYRTMMFSNEY"
     gene            1459..2055
                     /locus_tag="PC429_001885"
     CDS             1459..2055
                     /locus_tag="PC429_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847391.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIDDKTVDVIEKIFTCIED
                     EEIKNYLIRDNCKLQELVNTYNKLEDLNIDEAIFFAWYNLTINTISIDKASEYYDDLT
                     TSNYIEVENHIIYTNERDLRKYAEDELETMLSMEHHIDRLLHKDMLIDMWLNNTSKEE
                     VIEEILMSDDIEDILNLSPEYAFTLANGIEYRYSEKEE"
     gene            2208..2477
                     /locus_tag="PC429_001886"
     CDS             2208..2477
                     /locus_tag="PC429_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HDF3847392.1"
                     /translation="MTNNNNNCDYKGIYADMVEVLGEEIVIKLHKYYRGQQITFPMKL
                     YSIEYVERYIVKNYRTKTIKEMCRELGYTEGWIKQLINKNRLNSK"
     gene            2503..2745
                     /locus_tag="PC429_001887"
     CDS             2503..2745
                     /locus_tag="PC429_001887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847393.1"
                     /translation="MILTVLAILMGTGIFFLINSMLDITYFGCGPIGFLWFGCFIVSY
                     AILTSLGGIVLGLLKWIVIGLIILFVIGLIANKIND"
     gene            2951..4084
                     /locus_tag="PC429_001888"
     CDS             2951..4084
                     /locus_tag="PC429_001888"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012938.1,HMM:NF016983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF3847394.1"
                     /translation="MQDFDFISETIKKFQSDLENMDKVNILVVGKTGVGKSTLINNIF
                     REELAETGIGRPVTQHLKKISKEGLPINLYDTKGLELEEDVQETIKKEILDEIENCNR
                     NSLERNDKSELMHVCWYCINTSVARCEETDIKWINELSEKIPVIVVLTQGFPRKKARE
                     LKSSIDDLNLNCKNVIPVLAEQLVEEEDEIVIKSYGLDKLVEVTYQVIPKEVKKAFVN
                     VQKVDIKKKVEESRKWVLGYVGGSGAIGFSPIPFSDAPLLATAQVAMISHITVIFGLQ
                     VDKALLTAIVSSIGGVTSATLLGKTLVSNILKFIPGAGTIAGGAISGSVAALTTTALG
                     MAYISVLEYILNENMKGNEVNNTEIAEKMKDAYKDQLGMLSKK"
     gene            4848..5093
                     /locus_tag="PC429_001889"
     CDS             4848..5093
                     /locus_tag="PC429_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847395.1"
                     /translation="MRSVTVEDGDERFAIEKIYIKSLQRYEVRICLYRDCRDRINKLI
                     PRPVDLTMDKFVALILVGLKAGVLDDDFKQELIKGLA"
     gene            5441..6802
                     /locus_tag="PC429_001890"
     CDS             5441..6802
                     /locus_tag="PC429_001890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847396.1"
                     /translation="MKGNINKKDIDLSKITFDKFLEDEGLQNRERDYLYVDDYDNTLD
                     TQEFKLHIIEENTDNTYIDKDKYWYLDLDDSLFEYEEDEDTIAKTLYSLIDIRIKFLK
                     HVEYRKYINLGVIDEEYFDEIKSFLKFYIVAKTKKNELGNSNLKQMAIDIFDKERFED
                     DISDDDMENISEWIGEDQNFKMFYDKVCRDLSNSTQDKEILKLAGIRNNKVKHYKGRK
                     ISRKCKLELKSLTKMWEYETKLTHDANKAHKKIKSNMLEKMTDKNYKNIYEYLNNEII
                     NSNEEYKNIYFYSLELKLANELRKCTLKNLNKIKDIENQVKFISYTIAYIHIVPVLTI
                     REKLSDKLFNILSTNEGKDIEKFTKEVSLIMRIINIECLELLNIVSKNNYYKNFSEKE
                     LEEFKSIYNINNGRKSFENKYKIKVDYNIDDVRKYGEVIKAINIESKKKIVDRIKKKR
                     KSN"
     gene            6992..7309
                     /locus_tag="PC429_001891"
     CDS             6992..7309
                     /locus_tag="PC429_001891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847397.1"
                     /translation="MTNFKSVKVELILLIECEEGNRNELEGMIDEGVLNEKEYSLTIL
                     GSTEYEDNARAIYILMNTGGSYEKNLQRLSRLHLKIENLLKDTNVKYRGISLVPNIVK
                     WDK"
     gene            7321..9084
                     /locus_tag="PC429_001892"
     CDS             7321..9084
                     /locus_tag="PC429_001892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847398.1"
                     /translation="MSKAINREELIKLYEEIGLNVLDNNTVMNSSNKKICNFLVNIIE
                     QRVIDGKMYLCINAMGINGELLSEVILDSNTINRTTWVIENWGFKYSIDKKYINDFIE
                     LIQHLCMNIKTKYIYDHTGWIYQDNKWIYLHGGGVIGGSDVQVELDESIDNYILPDKI
                     SDINKACEMSLKLVDLIEDDKGIIFSSLVYLSPLIEIISRKIKPPENIVWTYGKTGSR
                     KTAVSKVVLSHFGDFNKKIPATFNDTITAIELKANKLKDSLYLCDDFAPKQDFRDKKI
                     QDSKAESIIRMYGDRVGKGRSSNKLDIKDAKRPRGMILITGEDVICGESSNARLLSIE
                     LNRSSVDLNILTELQNNSHLLGEAMRGYIEWLLSEMGNIDELSNILLYNFKSYRDEIR
                     EQYKDDIHGRTIESSAWILVGFSCMLNYIRDKGVIDKYEDDEYMDRAIKVVERLIEEQ
                     IKLISRNSPIDIFLNTLKESINSKSIRIATLNDQNQVIEDKLEYICGYKDKEYYYFYT
                     DKVYSFIEFENKKRGIHLGISQRQLVKQLRDSGIVKVDSNNDSPKKTIHTKDASDSTG
                     YTTIRPRMLQIERSIIENFQI"
     gene            9147..9302
                     /locus_tag="PC429_001893"
     CDS             9147..9302
                     /locus_tag="PC429_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847399.1"
                     /translation="MTETYKISIGRKDLYSKDYKVVERTDYEKRHTVNISRSLVDILS
                     ILVKEDR"
     gene            9389..10954
                     /locus_tag="PC429_001894"
     CDS             9389..10954
                     /locus_tag="PC429_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3847400.1"
                     /translation="MTGKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQF
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNILGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEEEIIKIEGGMPQIIDKGVFKQAQ
                     DMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNEYVSYRCGCRKQK
                     RECNNREIKRDYLEEFILQELEKNILNDEAIPILSKALNEKLNDKNESNAELLKNLEQ
                     KLDKVNKEISNILNAIMNGIVNSMLKDKLDELEQVKFNLDLKMNELKIENKVVDGVGV
                     TEDQIRGMFSRFKEFVLERNIPECKKFIGDYIKEVIVYKDHIEVIFNVVFCFVDNDIT
                     HDLHIITERGKIT"
     gene            11100..12392
                     /locus_tag="PC429_001895"
     CDS             11100..12392
                     /locus_tag="PC429_001895"
                     /inference="COORDINATES: protein motif:HMM:NF014177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF3847401.1"
                     /translation="MADIFTVLNYQGSKKRLLDFIYENTQEYIDSDKAFLDICSGTSA
                     VAYNMKRHFTVYTNDSENYAYQISNSIVKNNERYNFSQSDEMIIREKYNINLQRLNNI
                     FNDMVNQEDMLLEQEDADGLIELYSNCPCIWKNNVDIEIEGNTIQSIYDLRKCKRDIP
                     NVLFTLYYSSSYFGIKQSMEIDSIRYAIEFIESEITKSILFTSLFFAMKECVFAKDGH
                     MAQPLNMETGKKRMLRLKARSIYDIFIDKLKEFNTEEFVLSQKENKCFNKDFRTLLET
                     EESIKDEVGFIYIDPPYTDMQYSRYYHILNTVVLYDYPEISLSRGTISKGIYREGRYQ
                     SPLSQRKHALNEIDFLFSFCKENKINVALSYAYPIDTEKQATNRYTASIDDLINVAKK
                     HFGDNVKVVHEEYSHANNRNSDKKNVLEYLIMGIHNKE"
     gene            12395..14014
                     /locus_tag="PC429_001896"
     CDS             12395..14014
                     /locus_tag="PC429_001896"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF3847402.1"
                     /translation="MDKINKLKERIANIEATNKNDIYSSNLYWSQKPFNICDEIIEHL
                     SEDGDIIFDPFMGSGVTVIEALKKEYKRNAIGCDINDAPIFIVKTLLEKYNVPKIEKD
                     IKELNNKLSKLSTYYSTKCRECGEVVTISKTIFDKENRNAKCELKSIHYKCKECKNDF
                     KGEPNETEISKFFIDEELKNIDDFQFIANSRLAVGEGDTVRTIFSNRNLIVLDKILGH
                     IDEYKNSESYEPMKYILLSILHLSKITDTHSNSQWPLWIPKKDCVEKNVVELFIKNSK
                     KTIKALKLASKYLCENRENVELIKNLDRGKFKLIKDGVQNIEDDVIPNEFVDLIITDP
                     PYMGQVLYSEYMQLYKPFMDLEFELENEIVISSAPARDKGKVLYFDMMNSAFENISRV
                     LKVGKYMCMYFHDSNLDVWDNIIDIMTNNNLKYMGQIHVSKNKNTLKNILSPKKSLNG
                     DCIIFFKKDLNINYDINEVDNIEDKINNIAQAIIDIKGYASTPELYDNGALEYIISNG
                     KLKEISKKYKDLTSIFEQRFNWDKERGVWTV"
     gene            complement(14139..15290)
                     /locus_tag="PC429_001897"
     CDS             complement(14139..15290)
                     /locus_tag="PC429_001897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847403.1"
                     /translation="MKKLTIRLKDDFHKDLLDLVAFKNDSINSYVINLIKNDLSKNTN
                     LLRSYDNMIRDNITNVLITQKINELKAIQDGIFKEYKVNDILSNSKIYEVMTANSLNH
                     ILIPGHSGSRDAKDEFGNEFEYKHFKKSSSNHSWTFNDFSDTSIAKLKKAKAVIFAHI
                     NDVDFPYPGKLDWYYSIPGDIMCEYLLKYTQSIQNSRKMINVSPNQLESRLNASKIIP
                     SAQNGVYDKYLSAIYKASSELEELTGVKNLLTSNKFWELIVALELNHQVNPEQGGREG
                     AHDAYDSEGNPYEYKVSKNHAWNFQDISDNVLNKYYNDKAIILAVVDKENIEVEHIYS
                     TEPADVVPLLRKKLQEKINRCLEKGSPIRRLQVSLSKSDLTRVNAKKLY"
     gene            15796..16011
                     /locus_tag="PC429_001898"
     CDS             15796..16011
                     /locus_tag="PC429_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF3847404.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            16011..16490
                     /locus_tag="PC429_001899"
     CDS             16011..16490
                     /locus_tag="PC429_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF3847405.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            16496..16726
                     /locus_tag="PC429_001900"
     CDS             16496..16726
                     /locus_tag="PC429_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847406.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(16842..18008)
                     /locus_tag="PC429_001901"
     CDS             complement(16842..18008)
                     /locus_tag="PC429_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF3847407.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(17998..18750)
                     /locus_tag="PC429_001902"
     CDS             complement(17998..18750)
                     /locus_tag="PC429_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF3847408.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(18842..19333)
                     /locus_tag="PC429_001903"
     CDS             complement(18842..19333)
                     /locus_tag="PC429_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847409.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(19330..19528)
                     /locus_tag="PC429_001904"
                     /pseudo
     CDS             complement(19330..19528)
                     /locus_tag="PC429_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19585..20346)
                     /locus_tag="PC429_001905"
     CDS             complement(19585..20346)
                     /locus_tag="PC429_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847410.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENITIK
                     NNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            20493..20693
                     /locus_tag="PC429_001906"
     CDS             20493..20693
                     /locus_tag="PC429_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847411.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            20697..21011
                     /locus_tag="PC429_001907"
     CDS             20697..21011
                     /locus_tag="PC429_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847412.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            21033..21776
                     /locus_tag="PC429_001908"
     CDS             21033..21776
                     /locus_tag="PC429_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847413.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
BASE COUNT         9113 a         2240 c         3576 g         7400 t
ORIGIN      
        1 ttataaatat atattatatt aatgaatcta tattaaatag atagttgagt aagtagtaat
       61 accaaaaata ttaaaaagag atgttggtag aaatacgaac atctcttttt tagtgcaaaa
      121 aattaaaagg aggaagttaa tatgattaaa ttatggaaga aagaagattt aaatttatta
      181 ttagaatatc caaaggaagt agttgaaaat gttgataacg tcattaatat tcttgatgaa
      241 aactatggat gtaacagaaa attaacagat gatggtggat atgtgtgtat aattgaggat
      301 attaaagaag tagaaaactt aaaatcaaat atattaaagg ggttagtaga agagtttagt
      361 gatgtaatat atgaagatga agttaataca tataattcaa cactatactt gttatctagt
      421 gattatagtg ttactgtaat ttctaaaaat gaagaaactg agtacttact taaataagca
      481 tatacattga taaggtgtat atgcttattt taattttaat aaataaagga gaaaagcaga
      541 atgaataaaa agagaactcc tgccaagaga gttgatatag ttttacttaa attagtaaaa
      601 gaacgtagtg ttctatatga aactagaaaa ataagtaatc tttatgatgc ttatagatta
      661 gcaaagaatt ttttataaat agtgctagag aaaagtttgt agtggtatgt ttagatacaa
      721 agaattggcc agtagcatag aggttgtaag tataggcact gtaaatttat ctatggtata
      781 tccaagagaa atatttaaag tagctatatt aaccaattca tctgaaatga tatgctttca
      841 caatcaccca atgggtaata ctgactttag caaggaagat agttataact aatatgctac
      901 agaagcgtgg agaaatatta tgcattgaat tagtagatac atatagttat tgtagatgag
      961 gataaatact ttagctttaa agaaaactgt aggatataag agtcttagct taaataaatt
     1021 aggctaaggc tttttctatg ttccaaaaca attaaaaata aatcaaattt aggttaagcc
     1081 cggaaagccc ttgaaaaaaa taatacatat aaatttaaaa ctaaatagga gttgtaacat
     1141 gtttaaaaaa gaaaataggt atatgacaaa agagatagct gaaaatctac caattgaaat
     1201 agctatacta ctatggaatt taatagatga cttaactaca gaaaaagatt atctacaact
     1261 atttgaaata aatcctatag gattaggagt agttgagata gttcataagc aagaagtacc
     1321 tgaatatgaa gctaggatat atatacagaa tgataaaatt aaagataaat taaaaatata
     1381 tgttatagat aatgttgaat atagaacaat gatgttttca aatgaatatt agttaatgat
     1441 taaaggagag agagtaatat gataaataat aaatctgaaa taattaataa gttatttttc
     1501 aatgaattaa aagagttaat agataaatac aacaatatag atgataaaac tgtagatgta
     1561 atagaaaaga tatttacatg tatagaagat gaagaaataa agaattacct aataagagat
     1621 aattgtaagc tacaagagct agtaaataca tacaataaac ttgaagattt aaacatagat
     1681 gaagctatat tctttgcttg gtacaactta actataaata caatatctat agataaagca
     1741 agtgagtact acgatgattt aactacaagt aactatatag aagtagaaaa tcatataata
     1801 tatacaaatg aaagagattt aaggaagtat gctgaagatg aattagaaac tatgttaagc
     1861 atggaacatc atatagatag attactacat aaagacatgt taatagatat gtggttaaac
     1921 aatacttcaa aagaagaagt aatagaagaa atattaatga gtgatgatat agaagatata
     1981 ttgaatttat ctccagaata tgcatttaca ttagctaatg gaatagaata tagatactca
     2041 gaaaaagaag aataaaataa gtatatgtac tgaaaaatac gggctttaag ggcttataca
     2101 ttaaagtaca tatttgaaaa taccatagat tttacaagag aaaatcagaa tgaaactttt
     2161 tcaaatacta gttattatta attagtgcta gaaaaggagg tggagtgatg actaacaaca
     2221 ataataattg tgattataag ggcatatatg cagatatggt tgaggttttg ggtgaagaaa
     2281 tagtcattaa attacataag tattatcgtg gacaacaaat aacatttcca atgaaactat
     2341 attctattga atatgtggaa cgatatatag tgaagaatta tagaacaaaa acaataaaag
     2401 aaatgtgtag agagttagga tatacagagg gatggataaa gcaattaatt aataaaaata
     2461 gattaaattc aaagtaatta gagaaggaga ttgacagtag atatgatatt aacagtatta
     2521 gcaatactta tggggacagg tatatttttt ctgattaatt caatgctaga tataacgtat
     2581 tttggatgtg gcccaatagg atttttatgg tttggatgtt ttattgtgtc atatgcaata
     2641 ttaacatcat taggtggtat agttttagga ttgttaaagt ggatagtaat aggattgatt
     2701 atactatttg taataggatt aatagcgaat aagataaatg attaaattac tgtagattat
     2761 tttgataaaa taattattta atatattgag gcattacatg atagaagaat aaatataaat
     2821 gacttaaaat ctttaactta actaaacaaa aaatatatta tttattacaa aaaatgacaa
     2881 aaaaattaaa tattatattg tattatgaca atataaaaca ttggagttta taaattagga
     2941 ggaatatttt atgcaagatt ttgattttat aagtgagaca ataaagaaat ttcaatccga
     3001 tttagaaaac atggataagg taaatatact tgttgtaggt aaaacaggag taggtaaaag
     3061 tacattaata aataatatat ttagagaaga attagcagaa actggaattg gtagacctgt
     3121 aacacaacac ttgaaaaaaa taagcaaaga aggattacca ataaatttat acgatacaaa
     3181 gggattagag ttagaagaag atgtgcaaga gactataaaa aaagagatat tagatgaaat
     3241 agaaaattgt aatagaaatt cattagaaag aaatgataaa agtgagttaa tgcatgtatg
     3301 ttggtattgt ataaatacat cagtagctag atgtgaagaa actgatataa aatggataaa
     3361 tgagttaagt gaaaagatac cagtaatagt tgtattgaca caaggattcc caagaaaaaa
     3421 agcaagagaa ttaaaatcta gtattgatga tttaaattta aactgtaaga atgtaattcc
     3481 agttttggca gagcaactag tagaagaaga ggatgagata gttataaagt catatggttt
     3541 agataaatta gtagaagtta catatcaagt aataccaaaa gaggtcaaga aagcttttgt
     3601 taatgtacaa aaggtagata taaaaaaaaa ggttgaagag tcgagaaaat gggttttagg
     3661 atatgtagga ggatcaggag caataggatt ttcaccaata ccattttcag atgctccatt
     3721 attagcaacc gcacaagttg ctatgatatc tcatataaca gtaatatttg ggttacaagt
     3781 tgataaagct ttactaacgg caattgtaag ttctatagga ggggttacat cagctacttt
     3841 actaggaaaa acattggtat cgaatatact taagtttata ccaggagctg gaactatagc
     3901 aggtggagcg ataagtggtt ctgtagcggc attaacaact acagcattag gaatggcata
     3961 tataagtgta ttagaatata tactaaatga gaatatgaaa ggtaatgagg ttaataatac
     4021 tgaaatagct gaaaaaatga aagatgcata caaagatcaa ttaggaatgt tatctaagaa
     4081 ataattaatt caagataaag tataagatta attataggga aaaagtaaaa tttaaaggta
     4141 tatttggtaa aacataaaca aaaatgataa tggcgtcaat attatttgtt gtcatatgta
     4201 gtagcttagt atatatatta ttgagtgata ggtttaatca taagttaatt tacttagaga
     4261 aagatatatt agatttaaga aataaaaatt tgtaactagc taataaagga tcagtataga
     4321 aacagataaa caagaggata atgtagatat taaatctaaa aacactaata atgaagaata
     4381 taaaacttca aaactattta ggcactagat agagtatttg ctataatgaa taagctaata
     4441 gagaaattaa aacgtttata gtaagataac taattcaaat acatttagtg gacttcaagg
     4501 aacatttgat acactatcaa actatggtag ccaattgata gaccaatcat ctaaattgaa
     4561 tttatcaaat gttggatggt tagaaatatc tgatataatt acaagtatag gaaaggacta
     4621 ttagattctg aaaatatgtc atttgaagca ttaaatataa tgggaaaaat aggaaaattt
     4681 ataaattata ttgaaattaa ttatattaac taattaaata ttgatacata atgccttgct
     4741 acagttaaat gtggcaaggc attatttttg taaagaaagg aagaattaac atggcgaagc
     4801 aaagtaaaga ggaactattt gaaaaagaaa ctaaatattg caagataata agaagtgtaa
     4861 cagtagaaga tggagatgaa agatttgcaa tagagaaaat atatattaag tcattacaaa
     4921 gatacgaggt tagaatatgc ctgtatcgtg actgccgaga tagaattaat aaacttattc
     4981 caaggcctgt agatttaact atggataagt ttgtagcatt aatactggta ggattaaagg
     5041 ctggtgtact agatgatgat ttcaagcaag aactaattaa aggtttagct taggaggtag
     5101 aatatgacaa tagaagagtt tactagtgga gtaatgtttg ggatttcaat ttttagtgca
     5161 gtattaaagc aagtattaga aaataaaaaa tagtatacag gaggtatgag tatgattgga
     5221 tttataatag gaagtataat aggaattgga ctatgtatta gtatagagat actgatatcg
     5281 aaaaatgcca taatatacta agttatattt aaattattaa tagattcata tataatttat
     5341 ttaagccctt aaagcccaga taagttatat taaagtaaag tttgtattta tgtaaattaa
     5401 ataatatata ttattataaa tttgaaagga ctttataaat atgaaaggca atataaacaa
     5461 aaaagatata gatttaagta aaataacatt tgataagttt ttagaagatg aaggattaca
     5521 aaatagagag agagattact tatatgtaga tgattatgac aatactttag atactcaaga
     5581 atttaaatta catataattg aagagaatac tgataacaca tatatagata aagataaata
     5641 ctggtattta gatttagatg atagtttatt tgaatacgaa gaagatgaag atactattgc
     5701 taaaacatta tatagtctaa tagatattag aattaaattt ttgaaacatg ttgagtatag
     5761 aaaatatata aatctaggag taatagacga agaatatttt gatgagatta aatcattttt
     5821 gaaattttat atagtagcaa aaacaaagaa aaatgagcta ggtaattcaa atttaaagca
     5881 gatggctata gatatttttg ataaagaaag gtttgaagac gacatatcag atgatgatat
     5941 ggaaaatata agtgaatgga taggagaaga tcaaaacttt aaaatgtttt atgataaagt
     6001 ttgtagggat ctctcaaatt ctacacagga taaagaaata ttaaaattag caggaatcag
     6061 gaataataaa gtaaaacatt acaaaggaag aaaaattagt agaaaatgta aattagaact
     6121 taaatcattg actaaaatgt gggagtatga aactaaactg actcatgatg caaataaggc
     6181 acataagaaa ataaaatcta atatgcttga aaaaatgaca gataagaatt ataaaaatat
     6241 atatgaatat ttaaataatg agattataaa tagtaatgaa gaatataaaa atatttattt
     6301 ttatagctta gaattaaagt tagcgaatga attaagaaag tgtactctta aaaacttaaa
     6361 taaaattaaa gacatagaaa atcaagtaaa atttatttct tatactattg cttatataca
     6421 catagttcca gttttaacga taagagagaa actaagcgat aagttattta atatattgtc
     6481 aactaatgaa ggtaaggata tagaaaaatt tactaaagaa gttagtctta taatgagaat
     6541 tataaatata gaatgtttgg agttattaaa tatagttagt aagaacaact attataaaaa
     6601 ttttagtgaa aaggaattag aggagtttaa atctatttat aatataaata atggaagaaa
     6661 gtcatttgaa aataaatata aaataaaagt agattataat atagatgatg taagaaagta
     6721 tggagaggtt ataaaagcaa taaatataga aagtaagaag aaaattgtag atagaattaa
     6781 gaagaagaga aaatctaatt agattttctc tatttttttt gaaaaaaata aaaaatattt
     6841 ggtgaaaacg tttatcttta ggtgatatga gtagtcatta tttattatat aatcatattt
     6901 gtaggaacga gctaattaat aaaatctaag aacaatagct taatgatgaa aaagtacggc
     6961 ctttcgggct ttgacaacag gaggtatatt aatgacaaac tttaaaagtg taaaggtaga
     7021 attaattcta cttattgaat gtgaagaagg aaatcgtaat gaattagaag gtatgattga
     7081 tgaaggtgta ttaaatgaaa aagaatatag tttaactata ttaggtagta cagagtatga
     7141 agataacgca agggctattt acatacttat gaatacagga ggatcatacg aaaaaaactt
     7201 acaacgatta agtagattac acttgaaaat tgaaaactta cttaaagata caaatgttaa
     7261 atatagaggt atatctttag taccgaatat tgttaaatgg gataaatagg aggaatacaa
     7321 atgagcaagg caataaacag agaagaatta attaaattat atgaggaaat aggattaaat
     7381 gttttagata ataacaccgt tatgaattca tctaataaaa agatatgtaa ttttttagta
     7441 aatataatag aacaaagggt tatagatgga aaaatgtatt tatgtataaa tgctatgggg
     7501 attaatggag agctattatc agaggtaata ttagattcga ataccataaa tagaactaca
     7561 tgggtgattg aaaattgggg ttttaaatat tctatagata aaaaatatat taatgacttt
     7621 attgaattga tacaacactt atgtatgaat ataaaaacta agtatatata tgaccataca
     7681 ggatggatat atcaagacaa taagtggata tatctacatg gaggaggagt tataggaggt
     7741 tcagatgttc aagttgagtt agatgaatca atagataact atatactacc agataaaatt
     7801 agtgacataa ataaagcatg tgaaatgagt ttaaagctag tagacttaat agaagatgat
     7861 aaaggtatta tattctcttc attagtttac ctatctccac ttattgaaat tatcagtaga
     7921 aaaattaaac caccggaaaa tatagtatgg acatatggaa aaacaggatc aagaaaaaca
     7981 gcagtatcaa aagttgtatt aagtcatttt ggtgatttta ataaaaaaat tccagctaca
     8041 tttaatgata cgattacagc tattgaatta aaagctaata agttaaagga tagcttatac
     8101 ttatgtgatg actttgcacc taaacaagac tttagggata aaaaaattca agactcaaag
     8161 gcagaatcca ttataagaat gtatggagat agagtaggga aaggacgctc aagtaataag
     8221 ttagatataa aggatgctaa gagacctaga ggaatgatat taataacagg tgaagatgtg
     8281 atatgtggag aatcatctaa tgctagatta ttatcaatag aattaaatag aagttcagta
     8341 gatttaaata tactaactga attacagaat aatagtcatt tgcttggaga agcaatgaga
     8401 ggatatatag aatggttatt atctgaaatg ggaaatattg atgaattatc taatatatta
     8461 ttatataact ttaaatctta cagggatgaa attagagaac aatataaaga tgatatacat
     8521 ggaagaacta ttgagtctag tgcatggata ttagtaggtt ttagttgtat gttaaattat
     8581 attagagata aaggtgttat agataagtat gaagatgatg agtacatgga tagagcgatt
     8641 aaagttgtag aaagattaat agaagagcag ataaaattaa tatcgagaaa ctcacctata
     8701 gatatatttt taaatacatt aaaggaatca ataaattcaa agtcaataag aatagcaaca
     8761 ttaaatgacc agaaccaagt tatagaagat aaattagaat atatatgtgg atataaggat
     8821 aaggaatatt attactttta cacagataaa gtatattctt ttatagaatt tgaaaataaa
     8881 aaaagaggta tacatttggg aataagtcaa agacaactag tgaaacagtt aagagatagt
     8941 ggaattgtga aagttgattc taacaatgat tctcctaaaa aaactattca tacaaaagat
     9001 gctagcgatt caacaggata tacaacaata agaccaagaa tgcttcagat agaaagaagt
     9061 ataattgaga actttcaaat ataacttata atcaagagtg cttatgtact cttgattata
     9121 ttctatgata aatggaggat ataaatatga cagaaactta taaaatcagc ataggaagaa
     9181 aagatttata ttctaaagac tataaggtag ttgaaagaac tgactatgaa aagagacaca
     9241 cagttaatat aagtagaagc ttagtagaca tactttctat acttgtaaaa gaagatagat
     9301 agattaaaat atggttacac acttagtgtt agtgtgtagc ctttaatata gattaaaatt
     9361 agtatattta aactttggag gaaataatat gacaggaaag aaagcagtag tatatgcacg
     9421 ctttagttca gataatcaaa gggacgaatc aatagatgct caattaagag caataaatga
     9481 atatgcagat aagaataata taaaaatagt taatcaattt atagacagag caaaatcagc
     9541 aacatcagat aaaaggccag agtttcaaaa tatgattaaa ttctgtgaag cagatacaac
     9601 aggaatatct atggtaatag ttcataagct agatagattt tctagagata agtatgattc
     9661 agctatgtac aaacagaaat taaaagtaaa aggaataaga gttgtaagtg tattagagaa
     9721 cttagataat tcgcctgaat ccctaatatt agaatcagtt atagagggaa tggcacaata
     9781 ttacagtgct aatttagcga gggaagttgc taaaggtcag aaagaaaatg cccttaaagc
     9841 ccttcataat ggaggcgatg caccattagg ttatgatgta gcacatgata aaacatatac
     9901 agttaatgaa gaagaagctc aagcagtaaa gattatattt gatagatata ttaaaggata
     9961 ttcatatagt aagataatag atgaactaaa tatcttagga tataagacta aaagaggtaa
    10021 taaatttggt aagaatagct tacatggaat attaaataat gagaaatata caggagtata
    10081 tgtatttaat aagactcaga gaaaaggtgt agatggaaaa agaaatggac ataagcaaaa
    10141 gagtgaagag gaaataataa aaatagaagg tggtatgcca caaataatag acaaaggagt
    10201 atttaaacaa gcacaagata tgattcaaaa aagaaagaaa actccaggtg cacataaagc
    10261 tactactaca tatctattaa caggactaat taaatgtggt gaatgtggac atgcatttca
    10321 ggggaataag agaaaagatt cttatggaaa tgaatatgtt tcatatagat gtggatgtag
    10381 aaagcaaaag agagaatgta ataatagaga gataaaaaga gactatttag aagagtttat
    10441 attacaagaa cttgaaaaga acatattaaa tgatgaagct ataccgattt taagtaaagc
    10501 attgaatgag aagctgaatg ataagaatga aagtaatgca gagctactta aaaatctaga
    10561 gcagaaacta gataaggtaa ataaagagat aagtaatata ctaaatgcta taatgaatgg
    10621 tatagtaaat agcatgttaa aagataagct tgatgagcta gaacaagtaa agttcaactt
    10681 agatttaaaa atgaatgaac taaagataga aaataaagtt gtagatggtg taggtgtaac
    10741 agaagatcaa ataagaggta tgttttcaag atttaaggaa tttgtactag agagaaatat
    10801 acctgaatgt aagaagttta taggagacta tataaaggaa gtaatcgtat ataaagacca
    10861 cattgaagtc atattcaatg tggtcttttg ttttgttgac aatgatataa ctcatgattt
    10921 acatattatc acagaaaggg gtaaaattac atagtaaata tatattttat aaaagtaaat
    10981 atcagatatt atttagataa aaattatttt tgaaaatatt agttttaaaa aatatattta
    11041 tataattata aaataaaatg gtataataaa actttaaata tgataaagag gtgagactat
    11101 tggcagatat atttacagta ctaaattatc aaggtagtaa aaagagatta ttagatttta
    11161 tatatgaaaa tacacaagaa tatattgatt ctgataaagc ttttttagat atatgctcag
    11221 gaacgagtgc agtagcttat aatatgaaaa gacactttac ggtttataca aatgatagtg
    11281 aaaattatgc atatcagata tctaattcaa ttgttaagaa taatgaaaga tataacttta
    11341 gtcaatcaga tgaaatgatc ataagagaaa aatataatat taatttacaa agattaaata
    11401 atatttttaa cgatatggta aatcaggaag atatgttatt agaacaagaa gatgcagacg
    11461 gtttaataga gctatattca aattgcccat gtatatggaa aaataatgtt gatattgaaa
    11521 tagaaggcaa tacaatacag agtatatatg atttaagaaa atgtaaacga gacataccga
    11581 atgtgttatt cacattgtat tattcaagta gttacttcgg cataaagcag tctatggaga
    11641 tagattctat tagatatgct attgaattta tagaaagtga aataacaaag agcattcttt
    11701 ttacaagttt atttttcgca atgaaagagt gtgtttttgc taaagatggt cacatggcac
    11761 agccattaaa tatggaaaca ggcaagaaaa gaatgttaag attaaaagca agaagtatat
    11821 atgacatatt tatagataaa ttaaaggaat ttaatactga ggagtttgta ttatcacaaa
    11881 aggagaataa gtgcttcaat aaagacttta gaacattatt agaaacagaa gaaagtataa
    11941 aagatgaagt tggttttata tatatagacc ctccgtatac agatatgcaa tactcaagat
    12001 attatcatat tcttaataca gttgttttgt atgattaccc agaaatatca ttgagtagag
    12061 gaactatatc aaaagggata tatagagaag gtagatatca atcacctctt agtcaaagaa
    12121 aacatgcatt aaatgagatt gatttcttat ttagtttctg taaggaaaat aaaataaatg
    12181 tagcattaag ttatgcatat ccaatagata cagaaaaaca agcaactaat agatatacgg
    12241 ctagtattga tgatttgata aatgtagcca aaaaacattt tggagataat gttaaggtag
    12301 tacatgaaga atatagtcat gcaaataata gaaatagtga taaaaagaat gtacttgaat
    12361 atttaataat gggtatacac aataaggagt aaatatggat aaaattaata aattaaaaga
    12421 gaggatagct aatatagaag cgacgaataa aaatgatata tattcttcta acttatattg
    12481 gtcacaaaaa ccattcaata tatgtgatga aattattgaa catttaagtg aagatggaga
    12541 tataatattt gatccgttta tgggatctgg ggttacagtt atagaggctt tgaaaaaaga
    12601 gtataaaaga aatgctattg ggtgtgatat aaatgatgca ccaatattta tagtaaaaac
    12661 attattagaa aaatataatg tgcctaaaat agagaaagat attaaagagc taaataataa
    12721 attaagtaaa ttatcaactt actattcaac aaaatgtcgt gaatgtggag aagtagttac
    12781 tatatcgaaa actatattcg ataaggagaa taggaatgca aagtgtgagc taaagtctat
    12841 acattataaa tgtaaggaat gtaaaaatga ctttaaaggt gaaccaaatg aaactgaaat
    12901 atctaaattc tttattgatg aagaattaaa gaatatagat gattttcaat tcatagccaa
    12961 ttcaaggctt gcagttggcg aaggtgatac agtaagaact attttttcaa atagaaattt
    13021 aatagtatta gataaaatat taggacatat agatgaatat aaaaatagtg aatcttatga
    13081 gcctatgaag tatattttat tatctatttt acatttaagt aagattacag atacacattc
    13141 taattctcag tggcctttat ggattcctaa aaaagactgt gttgaaaaaa atgtagtaga
    13201 attattcata aagaatagta agaaaaccat taaggcatta aaactagcat ctaaatattt
    13261 atgtgaaaat agagaaaatg tagaactaat caaaaatcta gatagaggga aatttaaatt
    13321 gataaaagat ggagttcaaa atatagaaga tgatgttata cctaatgaat ttgtggactt
    13381 gataataaca gatccaccat atatgggaca agttttatat tcagagtata tgcagttata
    13441 taaacctttt atggatttgg aattcgaatt agaaaatgaa atagttatat caagtgcccc
    13501 agctagagat aaagggaaag tattatattt tgatatgatg aattctgcat ttgaaaatat
    13561 atctagagta ttaaaggtag gtaaatatat gtgtatgtac ttccatgata gtaatctaga
    13621 tgtatgggat aatataatag atataatgac caataataat ttgaaataca tgggacaaat
    13681 tcatgtaagt aagaataaaa acactcttaa aaacatactt agtcctaaga agtcgttaaa
    13741 tggagattgt ataattttct ttaagaaaga tttaaatata aactatgata taaatgaagt
    13801 agataatata gaagataaga taaataatat tgcacaagct attatagata taaaaggata
    13861 cgcatcaact ccagagttat atgataatgg agcactagag tatataatat caaacggaaa
    13921 actaaaagag atttctaaaa agtataagga tttaacaagt atatttgagc aaagatttaa
    13981 ttgggataag gaaagagggg tatggactgt ataataaata aaaagcatct atagataatt
    14041 atagatgctt tttatttata ttttatagta aactaaatat ttaataattt ctaattttta
    14101 tacaatatgt taaaggattg gctatccaaa gatattattt aatataattt tttagcatta
    14161 actctagtta aatcactttt agataatgaa acctgtaatc ttctgatagg agaacccttt
    14221 tctaaacatc tattgatctt ttcctgtaac ttcttacgaa gaagaggaac tacatctgct
    14281 ggttcagttg agtagatatg ttcaacttct atattttctt tatcaacaac tgctaatatt
    14341 atagctttat cattataata tttatttagt acattatcag atatatcttg aaagttccaa
    14401 gcatgatttt tagaaacttt atattcataa ggattacctt cactatcata ggcatcatga
    14461 gcaccttctc gtccaccttg ctctgggttt acttgatggt ttaattctaa tgcaactatt
    14521 aattcccaaa acttattact tgtaagtaaa tttttaactc ctgttaattc ttcaagttca
    14581 cttgaagctt tataaatagc acttagatat ttatcataaa caccattttg agctgatggg
    14641 attatcttac ttgcatttaa tctagactct agttgattag ggctgacatt tatcattttt
    14701 ctggaatttt gtatactttg agtatacttt agtaaatatt cacacattat atctccaggt
    14761 atagagtaat accaatcaag ctttccaggg taaggaaaat caacatcatt tatatgagca
    14821 aatataactg ccttagcttt ctttaatttt gctatacttg tatccgaaaa atcattaaac
    14881 gtccaagaat ggttagagct acttttcttg aaatgtttat attcaaactc atttccgaat
    14941 tcatcttttg catctctaga accactatgt ccaggtatta gtatgtgatt aagtgaatta
    15001 gcagtcataa cttcatatat tttgctatta gaaagtatat catttacttt atattcttta
    15061 aaaataccat cttgtatagc ctttagttca tttattttct gtgttattaa aacatttgtt
    15121 atattatctc ttatcatatt atcatagctc cttaataaat tagtattttt agataagtca
    15181 tttttaatta gatttatcac ataactattg atgctatcat ttttaaatgc tactaaatcc
    15241 aataaatctt tatgaaaatc atccttgagt cttatagtta gttttttcaa gctatcacct
    15301 cggatattaa ttagatgtgc cgtcgagata agtataatta agcaggttta taaagtcaag
    15361 gattaaaaaa attaaattat agatatataa aagtatatat agcaatatag ataaatagct
    15421 aaactattaa tcgtgggtat tattcttgcg attcgtctcc accaattttg aaaaaagaat
    15481 gatgaaatac ttggaaatac tagtctttgt tgactagtat tttttatttt ggagaagaaa
    15541 tccaccaacc gtaccccaac cacagaaaat gaggtttttt tgggggtata ttggggtgca
    15601 tttaggctgt ataaaaagtt tttatttgtt tattatttta tttgtttatt attttatttg
    15661 tttattatga taataggtaa catagaattt taatatctac tttatgatat aatattttta
    15721 taaatgtaag acagtttaag ttgttaatta gtaaaaatat atattatata agcttgatag
    15781 gagttgaggt ttgtaatgga tgaaagaaaa ctagaatttt ctattttttg cattgaagct
    15841 ttagcagaaa aattgaatat agattcagaa ggagtttata aacttcttaa acatgatact
    15901 gatattttag ataactatat aataccttgc tatgaagcac tacattctca aagtaaaggt
    15961 tacatagtgg aagatttaat gcaagtactt aaagaaaagg gtgtgatata atgattgtat
    16021 atcatggttc atattgtgtg gtaaagacgc ctagtattac cttttcaaga gattctttag
    16081 attttggaaa aggattctat gtaaccagta taaagacaca agcaattaac tggacagagc
    16141 gttttaaaaa aagaggtaaa aaagcatatt taaatagtta ttctttagat attgataaat
    16201 taaaagaaaa ctgcaaggta aaaatcttcg atagttataa tttagaatgg cttgatttta
    16261 taatagaatg tagaaatatg agtgatatat atatgaaata tgacattata ataggtggta
    16321 ttgctgatga taagatttac aatacaatag aactgtatca agataatctt attgaaaaag
    16381 atgaagcttt aaaaagatta aaatactata aaccaaatga acaaatatgt ataaccaatc
    16441 aaaatgtaat agataaatat ttaaagttta ggtatgaaga ggaggtataa tgattatgga
    16501 agcaaataaa acattattac aaatactata taaaaaaatc atattagaat tttccaaaca
    16561 aacaggtaag agcttggaag aaagtatgga ttatttatat acttctgaaa catataaact
    16621 tataagtgag ggtgtatcag atatgcactg taagggtcat atatacttgg ctcaagaatt
    16681 aatgttagaa aatggattaa tgtaccataa gggctatcca agataaataa ttaaaactag
    16741 atacgaaata aggatacaat ataatataaa attaatttgt atccttattt tgattttgta
    16801 attaactata tgaaatcaca atatttatat gtatttgatt tttacaatga aaatatttta
    16861 tccaactttt ctacagcttc aactttctta tcttccataa cttgagtata tatattcaaa
    16921 gttattgata tatcataatg acccattaaa acttgtacag ttttaggagg aacatcattc
    16981 tcaaaaagtc tagtagcata agttttccta agaccatgaa actttatagg ctctatattt
    17041 aagctattta atatagactt taaattcctt aaaggtcttt tatcatctat agggatgcct
    17101 aattcattgc aaaatacata attattattt atatataatt ctccagcctt taatatatct
    17161 gtattttgat ttactttatg ctctttcaat ttatttaaaa catcttttgg aataggtaca
    17221 gttctgtaag aatttgatgt ctttggccct tgctctaata ctttaagctc tctatttcca
    17281 gttttatcta taaaatatgt tctttgaaga gttcttttaa ctgttaaatt agacttttta
    17341 aaatcaatat caaaccattt aagtcctaat aattctccta atctaagtcc agtaccaaga
    17401 gcaagtaaaa ataatacctc taatttatgc ccatctatag cttttataaa actcttttgt
    17461 tgttcaagag tcaatatttc aagtttgttt tcttttttct caactggtat atttaccata
    17521 gtacaatagt tacgttgtat aagaccttgt ttttcagctt ctgataagca ggttttcagt
    17581 ttagtgttta tttgccttat agtaggtata cttttattgg aatcaagcaa tctattgtaa
    17641 tatttttgta tgtgagatac tcttaagtac tttaatttaa cattgcctaa atcagtatct
    17701 ttaatataat tacgatagat gctttcatat ctttcaaatg acttgggttt taaatctttt
    17761 attctatatt caaataacca attgtaaaac caatgctcta aagttatttt atcatctgaa
    17821 atatcagagc ttaaataata ttgtttttta taagcatcta atttatcttt aacttccttt
    17881 tgagtcttac cataaaattc ttttctcagt ggtttaccat cttcattata gccaatcatt
    17941 atacgggaac gccaaccatc tttatatttt ccgatagagc cttctccatt agctctttta
    18001 gttgccattt ttaaacagct ccttatatga tttatagtta tatccatcaa agaatgtaat
    18061 acatttctct aataagaatt ttataacttc atcagcttct agtgtttcat agaagtttag
    18121 cattaatgtg ttgtactctt gtttatgttt agctggtatt gatagaactc ctatatcatt
    18181 atttataagt attaggtttg aggtaagtct agcagttctt ttgttgccat catagaagaa
    18241 ttggcaatag gacaaccata aatttattat tatagccttt tctaatgggt tagttatttt
    18301 atttatgata tttatgtcat ttacaaataa atcatttaat agagtggctt ctatgcattc
    18361 atattgagta gtaccagcta tacctatatt accatttctg aaatctccaa cgattagagc
    18421 ttcatctttt gcaactattt tatgtataga gcaaattgta tctttattta aagataagtt
    18481 ttctttgtta cttaatttta ttacataatc ccatgatgat tttatattta gtatttgatt
    18541 ttggtcactt aatttatgtc cgcctacggt tataccatca agtaatgttt gtacttctgg
    18601 gaatgtgaag gggttatttt ctaggttacc catatcatat acaaattcac ttatcattct
    18661 tctaatgtat gttaaagtat attttttatc tccatgaggt atattagtag ggaatttatg
    18721 tgtgtatttt ggtatatatg atttattcat gattttagct cctttcatat ctaattttta
    18781 tttattaact atgaatgtat aaaagttaat aggtaaactc atattttgaa aatgtgcaat
    18841 actaatattc ttcatcctca tcttcaataa gaccattaga gttttcttgt ataaaatttt
    18901 caacaagacc atttatcacc caatttagat ttttggagaa gtctttgaag tcagagaagt
    18961 ttaggttaat tggttttatt gtatctgcat tatctgtaat ttcaaacttt tttttatagt
    19021 ctgtttttat atcataacca taattttcta atatgtgaat tactaaatcc aactctttat
    19081 caaatacttt taaattaata ttattatttg ttaaagtatc taaatcattt tcttcaacaa
    19141 tgagtttatt tatagcaatt gataaagcag tagctatttt tcttatcata tctaaactag
    19201 gcattctttg attttgctca tatttagcta tagcatgaac tgaacaacca atcttgttgg
    19261 ctagctcatc ccttgacata tttatcgatt ttctatattg tttaatcttt tctccaattg
    19321 tattattcat cataatttca ccttaaatat atcatcaaac aatgattgta cttctaaaca
    19381 tgtaaaggag ttattttcta gattatccca tatcatatac taaattactc ataactctct
    19441 tagtgtatgt tagagtatat tttttatctc catgaaataa attagtaggg gatttatatg
    19501 tgtattttag catatatgat ttattcataa ttgtaactcc attcatagtt ttttatcatt
    19561 gctttagata gtattgatat tattttatat tttagttagt agttttttta tttctttaag
    19621 tgtattttca agcttttcat ttttttcctt tagttcgtta ttttcttgta ataatttaaa
    19681 gtaatcatta tttttaatag taatattttc tttttgagat attaattttt ttagtcgaat
    19741 attacttcca ttatctgttg agtcactaaa gtaatcatag attgtttcta tactttcttc
    19801 tgatagacca atatctaata attcatcaat tgattgtttt gtaagaacat catttttaat
    19861 aatttctttt atgtcatcag gatgtaatga aaaatctaat tcagtattag atatacaatc
    19921 tgaaagtatc caattataaa cttgattttc atttagttct aatttttcac caagtttata
    19981 atattggtct ttatcagacc aggcacctag caataaactt gaggtaattc taatatcatc
    20041 ttccataaga tttgaaatag catttaaaaa ttcatcaaaa gaatagtatt cttctgggaa
    20101 taaagactgt aaaaggtata aaacatcttc attagtcatt acttcatttt caatatcaaa
    20161 ataaaagata gtcttttcaa ttttcaaagc tttagcgagt tttgataagg tatctatact
    20221 aggtattctt ttatcatttt cataatttat tagagcattt cttgagatgc cactaatttc
    20281 agcaagttga atctgcgtta gctctttttg ttttcttatc ttttttaagt tttttccaat
    20341 ttgcaatatt atcacctcgt ataaagtata tcccaaaagt acacaaatga gaacagaaaa
    20401 ataatatatt cttattaaca gtacacatat atcgacatta taattaaaat gtaaagttta
    20461 cgaatgtaaa ctagaaaaaa ggaggaaaaa gtatgggaat caatttaaaa atatttagga
    20521 ttaaaaaagg tctaaagcaa caagaactgg cagatagagt tggtatatca agatattatt
    20581 tatcaaactt ggaaacaggt aaggctaata atccaagcaa tgatttgatg attaaactat
    20641 ctagagcttt agatacaaca gtagaagaat tattttttag taaggaggat taaaatatga
    20701 aagagaataa taatttagag gttttaaaga cattacaagc cactttaaac attattgtaa
    20761 agaatgaaga agaagcacaa gaaaaaaagc aaggatataa aattattgaa aatgcgaaaa
    20821 gaaataaagc tgagcttata acatgtaaag aagcatctaa gctttatcct ataggagaat
    20881 caaaatttag gcagttatgt cattctaaaa acaaaggatt cccttgtata tggataaata
    20941 atcgagtata cataataaaa aataaattag atgattggtt tattgaaaat gcaaatggta
    21001 tcaagttata gtcttaaagt aggtgtttgc aattgagcaa taaagggtgg ataaagcttt
    21061 atagggagtt gttggataaa tcaatttgga aagatattaa accagaaaga aaggtaatat
    21121 taataactct attacttatg gctagtcata aagaaaatca tgtttttata gataataaaa
    21181 agataaaaat aaagtttgga caatttattt cttcatatag caaaatagct gaaagatgtg
    21241 gtaaaggagt tagttatcaa atggttagaa gttgtttgtt ggtttttaaa aatgctggtt
    21301 ttctaacata cgaaacaaca aacaagtata tagtaataac tattgaaaac tgggagcttt
    21361 atcaagagaa tatagaaaat caacaaccta atcaacagac tattaacaat cagtcaacaa
    21421 caatcaagaa tataaagaat gaaaataata ttatatatag tgaaatcata agcaaattta
    21481 actcaacttg tattgaactt tcaaaggtca agaaactaac agatacaaga aagaggagaa
    21541 taaattcaag aataaaagaa tatgataaag aaactgtatt agaggttata aatatagtta
    21601 gtaaaacaag ttttctgaat ggaaataata atacaggatg gaaagcaaat tttgattgga
    21661 taatgaattc aagtaatttt actaagatac tagagggaaa ttataatagc aaggaaattg
    21721 tcataaataa agagacagga atatataatc cagatgactc acaattaaag tggtaaggag
    21781 gttatattat aaataattca aacaacttaa ttaaaaatac agaagcagag caatcaatta
    21841 aattacacta agttagttat aaatctttca tatatttgtt ttttaagtat ctctaatatc
    21901 gtttaaatta cactaagtta gttgcagata taccagaaaa tatatataaa attatgcgta
    21961 aactatattg ttatgagtac aaataatatt tacctattca ttaaaaaact taactaagaa
    22021 tttaatttat attattgaag tatcaataat tttggaaagt agaaaattat accccaataa
    22081 taccccaact acaaaaatag acaataaaaa ataaccagaa aaattgcttc aacatcatga
    22141 ccaaatagcc atatacctat acttataaaa accaacaaaa caatcaaata accacttata
    22201 aattctcctc ggtctccacc attggaatcc acgaaaacat attgatttaa tagttaatat
    22261 gttttttatt ttgaaaaatc atatataata taaattaata gtttatttaa aaatttcagt
    22321 atgagcaca
//
LOCUS       DAMKMI010000054        22274 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMKMI010000054 DAMKMI010000000
VERSION     DAMKMI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22274
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.055"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            139..768
                     /locus_tag="PC429_001909"
     CDS             139..768
                     /locus_tag="PC429_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847414.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            769..1551
                     /locus_tag="PC429_001910"
     CDS             769..1551
                     /locus_tag="PC429_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847415.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            1544..2422
                     /locus_tag="PC429_001911"
     CDS             1544..2422
                     /locus_tag="PC429_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847416.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            2455..3414
                     /locus_tag="PC429_001912"
     CDS             2455..3414
                     /locus_tag="PC429_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3847417.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3728..4420
                     /locus_tag="PC429_001913"
     CDS             3728..4420
                     /locus_tag="PC429_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847418.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            4430..5434
                     /locus_tag="PC429_001914"
     CDS             4430..5434
                     /locus_tag="PC429_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3847419.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFAP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKGVNSLIKM
                     "
     gene            5682..6533
                     /locus_tag="PC429_001915"
     CDS             5682..6533
                     /locus_tag="PC429_001915"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF3847420.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            6637..7383
                     /locus_tag="PC429_001916"
     CDS             6637..7383
                     /locus_tag="PC429_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847421.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            7383..9299
                     /locus_tag="PC429_001917"
     CDS             7383..9299
                     /locus_tag="PC429_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847422.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            9578..10495
                     /locus_tag="PC429_001918"
     CDS             9578..10495
                     /locus_tag="PC429_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF3847423.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            10573..11310
                     /gene="cas6"
                     /locus_tag="PC429_001919"
     CDS             10573..11310
                     /gene="cas6"
                     /locus_tag="PC429_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF3847424.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            11316..13166
                     /gene="cas8a1"
                     /locus_tag="PC429_001920"
     CDS             11316..13166
                     /gene="cas8a1"
                     /locus_tag="PC429_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF3847425.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            13168..14082
                     /gene="cas7i"
                     /locus_tag="PC429_001921"
     CDS             13168..14082
                     /gene="cas7i"
                     /locus_tag="PC429_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF3847426.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14084..14785
                     /gene="cas5b"
                     /locus_tag="PC429_001922"
     CDS             14084..14785
                     /gene="cas5b"
                     /locus_tag="PC429_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF3847427.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            14796..17117
                     /locus_tag="PC429_001923"
     CDS             14796..17117
                     /locus_tag="PC429_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF3847428.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTYLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            17132..17683
                     /gene="cas4"
                     /locus_tag="PC429_001924"
     CDS             17132..17683
                     /gene="cas4"
                     /locus_tag="PC429_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF3847429.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            17686..18666
                     /gene="cas1b"
                     /locus_tag="PC429_001925"
     CDS             17686..18666
                     /gene="cas1b"
                     /locus_tag="PC429_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF3847430.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18669..18935
                     /gene="cas2"
                     /locus_tag="PC429_001926"
     CDS             18669..18935
                     /gene="cas2"
                     /locus_tag="PC429_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF3847431.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19119..20518
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19119..19147
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            20579..21007
                     /locus_tag="PC429_001927"
     CDS             20579..21007
                     /locus_tag="PC429_001927"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF3847432.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(21462..22241)
                     /locus_tag="PC429_001928"
     CDS             complement(21462..22241)
                     /locus_tag="PC429_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847433.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
BASE COUNT         9024 a         1993 c         3744 g         7513 t
ORIGIN      
        1 taatatttca tttaaaatat taaaaaatgt agataaaaat tgttttatta agaaaaaatt
       61 aatatattga caaaatttaa gtgctcattt aaactattaa ttgataataa tattcaatat
      121 taaataatag gaggcgttat gaataaatat cttctaataa atccagtggc agaaagaatg
      181 tatggagaaa agtttcacct tattaaagca aatttgataa aaaaggggta cataataaca
      241 gaatgtgaac cacagttaga atatgtaaaa aagcagtaca aagagtatac aaaaaagact
      301 gaaaatacaa tgttggattg tagatgtcca gaaagtataa gtcttttaaa aagaaataat
      361 ttgataaata attttgaagt acctatgata gaaccaatat tagtaaggac ttgtagggtt
      421 ttatacgata aatatataaa aagtaaagat gatatgttgg tagtaacctg tccatgtaca
      481 caattaagag actttgcaag tgaaaagttt aaggataagg gtaatgctat gttttataca
      541 tggaaagagt ttgctgaaca agaaggtgta aaatcacttg gaaaaataga tgaatcacca
      601 attccattag gatatttcaa agatactttt gaaaaagttc ttgaagtatc aagtgaagac
      661 gaaatattaa gtgaaataca aaaaataaga aatggttttg aagataagta tgacatagta
      721 gaaatgtttt attgtaaaga tggttgtaat aatggagatg gtttgtaaat ggtcaataaa
      781 tttaataata agtttttaag gctagtaaag agtgttctga tattttttat aattctttta
      841 ttatggaaaa ttacaaatta tcttggtatt tggagtgatt atatattacc atcacctgaa
      901 aaggtatata gtacattttt aaatatgata agtgatggtt caatatttat taatgtatat
      961 gcaagtatga aaagagtttt gataggtttt gcaataagta ctgctatagg tgtgccttta
     1021 gggatattct ttggaattta tagtggagta tacgaatact ttaaatctct aataaatttt
     1081 ttaaggaata cacctcccct tgctttaata ccaatgctta tattatggtt tggtatagga
     1141 gaggaatcaa aaattataat tatagtttta gcatcttttt tcccaatctt tacaagcaca
     1201 ttgaaaggga ttaaaaattg tgactcaaaa ctgattgaag tgggtagagt atttgagttt
     1261 tcaaagcttc agataatatt taaaataata ataccaaatg ccattcttga tattgctgtt
     1321 gggttaaagt tagctcttgg atatagcttt agagcaatta ttggggcaga acttgtagca
     1381 gcttcatctg gtcttggata cttgatatct gatggaaaag aaatgtctag aacagatgtt
     1441 gttattgtag ggataatcgt aattggactt ttaggaataa ttacagatta catattttcg
     1501 attattgtga agaaggtaag taagggaaag atggtggaag catatgaata atttagtatg
     1561 tgactctgga tatataatta gaaatttata taaaagttat aaagttgaca ataaagaaca
     1621 tgtagttctt agtgatatct ctttaaacat agatagaaac catataacag taattttagg
     1681 tgaaagtggt tgtggaaaga ctaccttgct tagagcaatt gcatcacttg aaggtataaa
     1741 tagtggtgaa attaggttta ttaatgataa taaagagtat agacctaatg ttggttttgt
     1801 ttttcaggag agtagattaa tgccttggtt aaacgtaagt gaaaatatag cttttcataa
     1861 tcaaagaaaa aatactattt taaacaaggt gcttagaaaa tttaaacaca ataaaagaat
     1921 taggagtaaa attataaatg ataattttag aagcttagaa acaaatgata atttgaacaa
     1981 atcaaataaa tataatagga aaagtaatat tgatgtagaa aaatatctta agatgatgaa
     2041 cttgcagaaa tttaaaaact cttatccaaa tgaattgtca ggaggtatgg cgcagagagt
     2101 aagcatagct agagctcttt ctttcaatcc agatatgttg cttatggacg agcctttttc
     2161 ggcattagat tattttacaa ggattgatat gcaaaatgaa gttataagaa tacataagtc
     2221 aactaataaa ggcgtgatat ttgtaactca tgatattgat gaagcgttaa agataggcaa
     2281 gaaaatagtt gttttcacag acgaaagaaa agtaaaagaa tttaatatag aagatggtta
     2341 taatagagat ttaacttcag attactatat taaattaaaa aaagatattt taatgacaat
     2401 gaagtcaaat aaaggcattt aaagaaatta ataatatttt aaggagtaaa gtaaatgaaa
     2461 ttaaaaaagc tattgatagg tataacgata ttaacacttg caacagcatc tttagctgga
     2521 tgtgctaaaa aaaataatgg agaaaaactt tcagaaataa atttaactta tgtaaaatca
     2581 ccattaaatg ttccttctat aattcagaaa caggatgatt tatttggaaa ggaatttaaa
     2641 aaggataata taaaagttaa cttccacgaa attactacag gtccagagca aacacaagct
     2701 ttagctgctg gagaaataga cttcttacat gcactaggtg gtacatcagc tttaatagct
     2761 gcatctaatg gagtagagtt aaagatatta aacacttata gcaggtcacc aaaaggattt
     2821 atgattttga ctaataataa ttcaataaaa tcagcagcag atttagtagg taagaaggtg
     2881 gcaggcccaa aagggactat attacatcag gtattgattt cagcacttga taaagaagga
     2941 ttaagtatgg atgatgtgga atttgttaat atgggtattc cagaggcatc agcagctctt
     3001 tctgatggca gcgtagatgc tgcattgatt gctgggccag cagctcttaa agctatgaag
     3061 tcaggtagta aacttgtagc aaatggtgaa ggtttagtag atggtataat agtaactgct
     3121 gttagcacag actttgcaga gaaacatcca gaaatagttg aaagatttat gaaggttgaa
     3181 aaggaaactt tagaatatgt aaataataat tttgatgaag ctatggaaaa agtagctaaa
     3241 gaagttgatt taagtcttga agaaactaaa gaattatatg cttggtatga ttttagttta
     3301 gatataacag ataaagacat atcttcgctt gaagatacac aagacttctt aataaaaaat
     3361 aagttacaag agaaaaaagt gaacataaaa gaattaatct ataatgctaa gtagaaacta
     3421 tgataaaata atactaagta agaaatataa taaaagttta agttacctta ttaaggtaac
     3481 tttttctatt ttgaattagt aatttcaatt tattttagaa tgattagttg ataataatga
     3541 ttaattaatc ataaaaagat acttttttat agagttttat taagatgtct aataatatgg
     3601 ttatacttat gaagtcagta attgtgaagt tagtaatatg attaaattta tatattgtaa
     3661 aaagattata atatattaaa attggactgt tatatgatga gtaaataggt atattaggag
     3721 gaattatatg agttataaag tatatattgt ggaagatgac atatcaataa gtacattact
     3781 taaagattat attgagaaat atggatttga agtgattgtt gaagagaact ttgatgatat
     3841 aatgattaca tttgaaagtt ttaagccaca cttagttcta ttagatgtaa atttacctaa
     3901 atttgatggt ttctactggt gtagatgtat taggcaacag tcgaatttac ctataatttt
     3961 tatatcagct agagatggta atgtagacca aattagagct ttagaaagtg gagcagatga
     4021 ttatataaca aaaccttttt actatgatgt agtaatggca aaaataaaga gtcatataag
     4081 aagggtatat ggtgaatatt cacctaaaat agacgaaaga atagtggagc tagatggatt
     4141 aaaattttat ccagaaaggt tagaactaga attaaatggt gagaatctaa ttataactaa
     4201 aaaagaaggt atactgattg aatgtcttat caagagatac ccaaaagtag taagtagaga
     4261 ttatctttta gaaaagatat gggatgatat tgagtttgta gaggaaaata ctttaaatgt
     4321 aaatgttagc aggataagga aaagatttga ggaattaggt atagataatg ctgttcagac
     4381 tgtccgaggg ttaggatata aattaaataa gaattggtaa ggagaaagta tgaagttatt
     4441 tttaaaagat tataaaggtt atatttttat atattatata ggcatagtta tgacaattat
     4501 ttactgtaat cttatgaagt ttattagttt tggagagagt ttatatattc ttttatttag
     4561 ttcttttata ttgttatgtt ttttaatata taagtattat aaaaatagag aagtatataa
     4621 tctgtttgaa aaaggagtac attctttgga ggagtcattt ttgtatttag gaagctcatt
     4681 cttggggaga aatatatcag ttattttaaa acaacaacat aaattgtatc aatcaatgat
     4741 acaagagcat aaaaagatac atagtgaaca tttaactttc atcaaccaat ggattcatca
     4801 gatgaaaaca ccaatatcag ttataagcct tcagctccaa gattatgagg gagaagaagt
     4861 aactgaaaat ataagaaaag aaatagatag attgaataaa gggttagata tggctatgca
     4921 ttttgcaagg gcagataatt ttcagaaaga ctttatagtt gaaaaagcca aattaaatga
     4981 aattgtatct agtattgtaa atgaagaaaa aaggttattt ataaaaagtg gaatgattcc
     5041 taagattcaa gtagacaagt ttacttatgt ttatacagat gtaaaatgga tgaaatttat
     5101 aataggtcaa ttaattacaa atggagtgaa atattctaaa gattatagta actacctaac
     5161 tataaaatct gaagaaaata ataaatatgt aattttaagt ataatagatg aaggaattgg
     5221 aattgtttct aaagatttaa aaagggtatt tgccccattt tttacaggag aaaatggtcg
     5281 taagtttgga gaatctacag gtatgggtct ttatctagta aaaaaggtat gtgatgattt
     5341 aggtcataaa gtaagtataa aatcagaagt aggaaaaggt acagaagttt caatctactt
     5401 taataaaggt gtaaatagtt tgataaagat gtaaattatc tgttacaata gggtttttag
     5461 ttaattgtac ttttttatta tacaatttat taaatgaaat attcatagtt aaataatcaa
     5521 aatttaagag agtttatttg caaaccttac atagatgtaa ggtttgtgta atgtttgcag
     5581 atagttaaat tttctaatct aaattacaat taatataaga aataacataa gagaaataac
     5641 ataaaaaaag aaattttgta aaaaaggaag gatgtttatt gatgagttta gatgttattt
     5701 ttatattgaa atcagtcata atagctatag tagaggggct tacagaattt attccagttt
     5761 catctacagg tcatatgatt ttggttggta atttaataga ttttaagggg caatttgctg
     5821 aaatgtttga ggttgtaata caacttgggg ctatattagc agtagtagta ctttattgga
     5881 aaaagataaa agatagcgtt atagaatttt ttaaatttat atttactggt ggaaaagaag
     5941 gaaagattgg ttttagattt ggaatgaatg taattatagg ttgtatacca tttgcgatta
     6001 taggagttct attttacgat aatataaaat cattgtttaa tttacaatct gtaattatag
     6061 gatttatagt gggtgggatt ttattattag tagtagaaac tttgtttaga aaaaagaatc
     6121 attccacaga taatatagat aaaattactc caatacaagc tttaaaggtt gggacattac
     6181 aagttttgtc tgcatggcca ggtatgtcaa ggagtgcatc tactattatg ggtggatgga
     6241 tagcaggact taattctcct acagcagctg agttttcttt ctttttagca gtaccagcta
     6301 tggtggcttc ttcaggaaaa gatttatttg aatttgatta ttcaataatg acaccaacat
     6361 tgtggattgc tttggtagtt gggtttatag tagcttttat tgtatcaata atagttatgg
     6421 aaaaattcgt gaatttttta aagaaaaaac caatgagagt gtttgctgta tacagaataa
     6481 tcatgggtgt agtacttgca gtattagctt ttactaacat aattagtgta tagaaaaaat
     6541 agatttatta tctataaaaa ttaaactaag ataaattata attaacaatt atatatatgt
     6601 tatttcaaaa attaaattag gaaggatagt taaataatga aaattttaac tgtaaataat
     6661 ttaagtaaag tatatggtaa aaaaataatt ttcaacgcat taaatgacat aaattttagt
     6721 atagaagatg gagaatttgt aggaattatg ggtccatctg gtagtggaaa aactactctt
     6781 ttaaatatga tttctactat tgacaagcca actacaggaa ctatggaatt aaagggcaag
     6841 aatcctttat tgttaagagg tgaggaactt gcattattta gaagaagaga gttaggtttt
     6901 gtatttcagg attttaattt attggataca ttaactatag gagaaaatat agtattgcca
     6961 ttgacattag ataaggtcag tgtaaaagag caagatgaga gattgaatga agtatctaca
     7021 atattaggta taaaagattt attaggaaaa agaacttttg aagtatcagg tggtcaagct
     7081 cagaggactg ctattgctag agcattgatt aacaatccat caatactttt agcagatgaa
     7141 ccaacaggta atttagattc aaagtcatca aaggttgtaa tggagctatt tcaaaagata
     7201 aataaagaaa ataaagttac tactatgatg gtaactcatg acccattagc agcgagctac
     7261 tgtagtagaa tcttattcat aaaagatggg tctatatata atgaaatata taagggtagt
     7321 agtagagagc agttttatca agagataatg gatgtactta cattattggg aggcgataac
     7381 taatgaatat aaaacagttt gctattaaca atatttcaag aaatattaaa gcgtatttag
     7441 gatacttagc aagtattgtt atatcatctt cactgctatt ttcttttatt atgtttacaa
     7501 gtcatccaga tttagatgta tctcaatttc ctgattattt aaaagaaggt ctcaagatga
     7561 gtaagataat tgcatatcta ttcttattct tctttgtgtt ttattcagtt agtgtgtttc
     7621 ttaaaagtag atataaagaa tttggaatac tttatattac aggtatatca aagcgccaag
     7681 taatgaaatt aatctttata gaaaatatga taataaatat agtatcttct gtaattggaa
     7741 taataatagg cttaatcttt gcaaagatat ttttagtggt tatgtctact tttttagaat
     7801 tatcaccatt aaaattctat ataccattta atgcgatatt gtctacttta atctatttta
     7861 tgattttagc tgtattaaca tcaatattta cttcttttat agttaaagaa aatcaggtgt
     7921 tgaagttact taaaggtaca aaaactccaa aaccagagcc taaaacatca ctaatgcttg
     7981 ctatattaag tatttgtttg tttataatag cgtattataa tgctattacc tcaaccgacc
     8041 aatatacaac atatctgcgc ttagttccag ttacatccct tactatagta gcaacttact
     8101 tgttcttttc tcaatttagt gtatttttta tgaagaagct aaaattaaac aagagatttt
     8161 atagaaaaaa tacaaatatg ctttggattt cgaacttgat ttataaggta aaggataatg
     8221 ctagaatatt tttcttgata actataactt ctgcagtggc atttactgct ataggtacag
     8281 tttattcctt ttggaaagat gtagagcgtc aaattaattt aatatatcct aacacaatat
     8341 attattcaac aatgacattg cataatgacc ctaaaaagcc tgatagcaaa gaaaaagaca
     8401 aggaaagaat gatgtttata gaaaataatc taaaaaaaga gaatgtagac tatacaagga
     8461 ttaatggtga atttaaaact gtatttccca agaaaagtga ttttaatgtc agaattatga
     8521 aagaatctaa gtacttagaa attacagaaa ctataggggt taagcctata agttttgaag
     8581 ataatgaatg tatatcatta ttatcatcaa gtttaccagg aaataagaac gtcaaagaaa
     8641 atattattgt agggaataga ggtttaaagg tggcaaaaca agtggaagaa tgtgttatgc
     8701 cagcgtatta caaaaatatg tatgtggtaa aggataattt ttatgatagt atagaatcaa
     8761 aatttatagt ggatagattt actgcatttg aagtagagga ttcaagtaaa actatagaga
     8821 tatgcagaga atttgaaaat aaatttaata atgagtcagg gatgcaacca tatttgtttt
     8881 tctctaaaga attaatgata ggaactggga aattaatgta ttctacattt atatttttag
     8941 caatatttat aggtttaatt ttctttgtta ctacaagtag ttttttatat aataaatttt
     9001 atatggattg ccaagttgat aagaaaaagt atgaacagtt aaataaattg ggtatgacat
     9061 ataatgagat taaaaaagct tcaacaattg agattggaat agtatttttg tttccttatg
     9121 tagtggctgt tatacattca atatttgcac ttactgcact gaaaaattct tttgatatag
     9181 aagtagcatc atcagctgtt ttggtaatgg gaagcttgtt cttggttcaa atagtttatt
     9241 ttttaattgt aagaaataat tatttaaaag agataaggtt aaatttagtc aatttttagt
     9301 tactattatt aaatgataat aaaaagggtt tagaattaat taatattact ggaattatag
     9361 atgtttattt tttcagtaat attttttatt gtattaacta ttaaaatttt gtacaaatat
     9421 aaaatataat atggtaaatg tcaaaattat aaaaaaattc tacttatata aaatataaag
     9481 aggttatatg tacatatgtg tagaaataaa taataaaaat atcaattaat gttattaggg
     9541 ggggttactc attagtaaat tagctaatac acttagaatg atgatattaa ttcaaagcag
     9601 aggcaaaatg aaatgcaaag aattatcaga agagttggaa gtaagtgaaa ggcaaataaa
     9661 aagttacaag atgtatttag agcaagctgg tatatttata aattcaacac ctgggattta
     9721 tggtggttat gaaatagata aatgtaatag catctcatta attaaattat tagatagtga
     9781 agtttcaatt ctagatatga taaattcaca gttagagtat aataatgata tttacaaaaa
     9841 tgaatttaat aatattgtag aaaaaataaa agcagtatta aatacaggag aaaaaagcga
     9901 tacatatatg gattatttta cagttcaagc acaacgtaat tgtgattatg agtctgaaaa
     9961 aaataagtgt aatgaaataa ttagagcata taccacaaaa cacaaatttt ggatagaata
    10021 ttattcatta aattcaggaa atagtgaaag aattgttcat ccatatggtt tatttaatta
    10081 taaatcagat acttatatgg tagctttttg tgaaaaaaga tttaagttta tagactttaa
    10141 attatgtagg attaaagatt ataaggtttt agaagaaaaa tataatgtag ataaaagttt
    10201 tagttgggat gaatatagta aaaatagtat aggaatttat aagggagaag aaattaatgt
    10261 tgttataaaa attagtcatc cattttcaac aattataaag gagaaagttt gggttaataa
    10321 tcaacaaatc atagaatatg atgataaatc tataatgttt aaagctaaaa tgagaggtta
    10381 tgaggagata aaaagttgga ttttaagtat gggagcttat gttgaggttg tagaaccaga
    10441 tagacttaga aatgatatat tgttagagat tgaaaaaatg aaaaaaattt actgattttt
    10501 tcaaataatt tatacttgga ccaatatact tcacggtcta gtgttaatat attaattgag
    10561 agaggtgata ttatgaaaat gaaaattgaa tttagtacag gttgtatacc aatatcttat
    10621 aattcgctct ttatgagtat tataaaagaa gcaataagaa aatctaatga agattactat
    10681 aaaaacatgt actattataa agaaaagaat aataaaaaaa ctaaaaattt tacattttcg
    10741 gtttatataa aaaagtatag tattgaaggt gacaatttta ttatagaaga taaagtaata
    10801 ttaaatataa gtactccaga tttagaattg ggttttcata tatacaatgg gttaatgact
    10861 tctaaaaaat gtttatataa agactacgaa cttacaagaa ttagaatcga tttatcaaga
    10921 gaaaagaagg taacagaaga aagggtacta tttaatgcat tgtctccaat ctgtgtaaag
    10981 tctaaagaag gaaaattctt ggagataact gatgatagat acatagaaga actaaactat
    11041 ataactaatg aagtggttaa aaattatagg gggaatggat taaaaagaaa gttagaattt
    11101 gaaaatatag gtctcaaaaa ggtagtagtt aaagagagtt taagagaatt taaaaaaata
    11161 acaggtaaag agtatcaata tattaatggt tacaaaggta aatttatatt gaaaggtgat
    11221 attgatgatt taaatcttat ctacaatcta ggaattggtt ttagacgtgc tcagggattt
    11281 ggagatgtag atattttaga atggaggtga gataaatgaa gttaagagtt tatagaggag
    11341 attggttttt taatatgggt atagttggat ttttaaatat aataaagaaa gcagataaac
    11401 aagctgaaat atttattatg gaagattata tcgaatttga tagcttattt cttgaaaact
    11461 ttcatgagta ttattttaat tactttatgg atgaatatga tgtatcaaaa agaattaaaa
    11521 aaaatattga ttacagtata aattttatta aatctaagcc tgatagaatc aaagatggaa
    11581 ttaagaaaat taaagatagt gtgaaacaac aaaatgataa gattaaaaaa tttgatgaag
    11641 aaaaatttaa catgataaaa gagaagctag atagtatgtc taagataaaa agttatgatg
    11701 aatttgatag tttagaagct ttagtagatg agactataga tatatttaaa ataaaatcaa
    11761 taaatgacag gcttacagca aatctttata aatatgtagt tcaagataat tattttggac
    11821 aagttagttt ttttaatgtt gctaaggcga aattagattt agatggatta aaacaggtaa
    11881 tgtttaatga ttatttaaga caaattatat attttggaga gttagaagat ttattagaag
    11941 agaatgacta tgacaagttg aaaaactatt taaatgatag attaaatagt atagctaagg
    12001 atataaatga aaaaaaagtt agtaagtcta gtataaatac tatagaaaaa attatgaagg
    12061 aaataaataa aaactttata aagaaaaata aaagcataga agatataaaa aagtatatgg
    12121 atagtttaga agtatgtgaa atgtgtggtt tatataaggg aatattagat gaatactcag
    12181 aaagtaattt tgctccactt ggagtaagca ccaataatgc taggaatatg ttttggaatc
    12241 aggcgtatgt accatcaata tgtgatattt gtaagctaat attgttctgt actccagcag
    12301 gggctactta tacaagaaag aattatttaa ttaatgaaga aaatgagttt tatttatttg
    12361 ttaacatgga tacttgtata aatgagcttt ttgaaagaaa taatagttta aaagctcaaa
    12421 aaagtgagta ttcagattcc aaagatgaaa atccttttaa tcagcttata aaaagtatag
    12481 tagaagaaaa tacattaaaa agtgaatggc aacttcgtaa tatacttttt gtggaattta
    12541 aagcaagtat agattctaaa aaatgtaaaa taaattattt taatattcca acttatttag
    12601 caaagttttt tgtaaaagaa aataaaaaaa tacaaagtat atatgattat aaacttaaat
    12661 caaatgtgtt agatttaatt ttaaaaagta aagacttaaa acatttaatt aataacactt
    12721 taaggaatat agttaagagt aatttagaaa gcaataataa atcaaacata tcagggttat
    12781 cttgtttcaa tgttgttcaa ttaagaacaa tattaaataa ctataagaga ggagtgtata
    12841 aaatggacta taaaaagttg agaaagattt atgagtcagg ttgtgaaata catgattatt
    12901 atattgataa gaatgctaaa aataaaatag aaggaataac atataaaatt ttaaatttaa
    12961 ttaaggttgg taataaaaat gattttatgc aaacaatttt aagaattttt tcaccaagtg
    13021 aaaaaacacc accagaagaa attttacaga tatttgttga agaagattta gattttgaat
    13081 ctgtagggta tgcatttgta acaggattaa tttctggaaa atatgaagga aaagataaac
    13141 taccaaacga aaaggaggaa aaataagatg aaaagagggt taacatttac agtaatagca
    13201 caggctcaaa gtttaaacta tggggaaggg ataggtaata tatcagaatt aaaaaaatta
    13261 agtagagatg atggaaatat atatacattt gcatctcgcc agtgtttaag atatgacata
    13321 gtacgattag cagcagaatt gtttaattgg aatttacagg ttgtcgataa agaaaaaggc
    13381 actattcaat ttaaagacaa tataagtata agagaatcag aagaaatgga tttatttgga
    13441 tatatgaaaa caaataaaaa agatgagaaa gataaaaaag gaggttcttt gactagagaa
    13501 gctactgtaa gattaagtaa tgcaatttcc cttgagccat atagaagtga catggacttt
    13561 ttaaataata aaggattagc agatagaata ggagaacatc ctaatcttgc taacatagag
    13621 caacatttaa gttattatac atatacagtg actatagatt tatctaaaat aggtaaggat
    13681 ggagatatag aacttgataa taaagagaaa tgtagaagag tagtagaatt tttagaaata
    13741 ataaaagtat tgaatagaaa tattagaggt agacaagaaa atttatctcc tctatttgta
    13801 gtaggaggag tatatgaaat agcaaatcca tttttcttgg gtagaattaa attaaaagga
    13861 gataaaaatg gatttaaaat aaataagcaa gctatagaag aagtaattca aggtactttt
    13921 ttaggaaaag atttaaaaga atttacctat gttggaatgg tagatggggt atttataaat
    13981 aaagaagaat ttaagggatt gtttgaagat aattttttaa gtgtagataa attttttgga
    14041 caactagtta aagaagtaaa ggaatattat ggggtgaatt aatatgaagg ttttgaaatt
    14101 agatttattt caagaaactg catgctacaa aaaaccattt gctatgaaga ttacagagac
    14161 atatccactg cctccatatt ctacaataaa tggattaatt cacaaaatat taaatgctac
    14221 agaatatata ccatttaata taagtgtaca gggaacatat gaaagtatat ttaataatta
    14281 ccagacaaca tatttttata aaaaagatag tattacatct atgccaatga atagtcatat
    14341 gctattgaat gtaaatttga taattcatat aggtggggat tttgatttat tggaagaaat
    14401 atatgatagt ttattaaatt atgacgagca cttatcatta ggtagaaaag aagatttagt
    14461 aagaattaat gatattagat ttgtagaagt caataaattt gaagtagatg aggatataca
    14521 attagatgat tatgaaaatt ataagttatc agaatgtaat ataagaatgc ctatatatat
    14581 accaaaaaca agtttacaag atacagatat agatggtata agttacagac tcaataatta
    14641 ctatgataat aatgctgtgg aagataaaaa aagggtttgg aataaggtag atagttatta
    14701 tgttgaggat ggaaatacta tatcaattgg agatatatta ttagataatg aaggagattt
    14761 attatatttt aataatataa attaggaggg agattatgtt atatgctaaa tctaatccag
    14821 tggaaacact tagagaacat actgatgaac ttttaaaaca aatgaatgta ttaagagaaa
    14881 gttatggaaa aaatataaat agcttagatt ttctagaaga ggagatattt tggcaattac
    14941 ttgactttgt tatagaattt catgatattg gaaaagcatt ttcaccgttt caagagttaa
    15001 taaaaagcag aatggatact aaaataaatg aacctaaaat agtaacttat ttagaaaata
    15061 atgtaggcca taattatctt tctcctgcat ttattgatta tagttatata gacaggaaaa
    15121 aaaataaaga acttagagca gtattaaatc aagttattgt atatcatcat gaaagagata
    15181 tctttataga taaagatttt aaaaatttaa tacaaaaaat attagatgaa gatttaataa
    15241 ataaagttta tgaactacag catgaattta aagttagata tccaataaaa acagaaaaat
    15301 tgagtaaagt atatctacaa agtgtagaaa aaagaataga taaaaatcac aagtactata
    15361 atttatatat tatgttaaaa ggaatactac atagactaga ccatagtgct tctgctcatg
    15421 aagttgtgga atgcaataat gttataaata taggagaaca gacagaaaat tatttaatga
    15481 aagagtttgg tagtttgaga gaggctcaat catttgctaa atctaataga aataagaata
    15541 taatactgat tgcatctaca ggaatgggaa aaacagaaac tgccttaatt tggatagata
    15601 aagacaaggc attttttaca cttccattaa gagtaagtat aaatgcatta tttgatagag
    15661 caaaaaatat agttggtgtt ggagaatcaa atgatacatt cttaggtttg ttacattcaa
    15721 cagcaataga ttatctagaa gaaagtaatc aagaaaattc aagtgagatt gtagatttag
    15781 caaagttatt atcttgcaaa ctgacatttt ctacaataga ccaaatattt aaatttcctt
    15841 ttttgtatag agggtatgaa aaagtgtact caacattagc atattcaaaa gtagtaatag
    15901 atgagataca agcatattca ccagaaatag cagctgtatt ggtaaaaggt atagaaatga
    15961 tacataagat aggtggcaga tttatgataa tgacagctac tatgccaacc atatatatag
    16021 atgaattaaa aaaaagaggt gtaatgaata gtaacttagc tgaattgaca tgtaatacag
    16081 aaaaaataag acattgtgtt tctattgtag aaaattcaat agatgaaaac ttaggtaaaa
    16141 taatacaaag tggaatgaat aaaaaagttc ttgtaatagt aaatacagtt aaaagtgctg
    16201 tagaaaaata tgagttaata gaagaaatag ttaaaccaaa aggaatagat gtcaacttaa
    16261 atcttttaca ttctatgtat atacaagaag atagagcaaa attagaaaaa tatataaaag
    16321 aatttgccaa tagtgataga aatggaattt ggattacaac acaattagta gaggcatctc
    16381 ttgatattga ttttgatgag cttcatacag aaaattcaac acttgacagt ctatttcaac
    16441 gatttggtag atgttataga agtcgtgagt atgaagaaaa tagcccaaat atatttatat
    16501 atacagaaaa agctacagga ataggtagta tttatgataa ggatattgta gaaaaaggac
    16561 ttgagctact gaaaactttt ataaatggaa aagaatgtgt gaagatgaaa gaaaaatata
    16621 aggtagaaat ggttaagata ctatactcta aagaaagtct agaaggtaca gcttttgaaa
    16681 agagatttac cagtgcttta aatattttag atactataac gccatatagt ttaggttcta
    16741 aagatgcaca aaatatactt agaaatattg aaggatatac agtaattcca gaggatatat
    16801 ataataaaat tgaagaaact ttaatcaaag attatgaaga gttgggacaa gagcttataa
    16861 atgcatatag taatgatgat agacaattga taaataaaat aaagtctaag agaaaaaaag
    16921 caagaagaga gataattaaa aaaactgtaa atttaccaat atataaagct aaacaaaatg
    16981 taattgacat acaggtgaaa ggtcttgaag atttaaaaat acttttatat aaatatgata
    17041 tatacgaaaa taagcataca aaacagatat ttggtaaagg tgtgcttata agtaatgaaa
    17101 tagaccaatt tatttgattt ttatattaac aatgtggcta aaatttacta aagaaggtga
    17161 ttttgtgcca atagatttag aagtattaaa agttcaaggt gtaaaaataa attattatta
    17221 tgtatgtaaa agaaaactat ggctattttc taaaggaata accatggaac aaaatagtag
    17281 tagagtattg tctgggaaga tagtacatga agattcatat aaaagaatga aaaataaaga
    17341 aatattgatt gatgatatct tgaagctaga tataataaag gatgagtatg taaaagaagt
    17401 aaaaataagt tcaaagatga aagaagcaga tgaaatgcag ttatactatt acttatacta
    17461 tttaaagaat agtatgggta tagagaaaaa aggtactata aattatgtta aggaaaagaa
    17521 acaagaagaa ttgatattga cagaagaaaa agaaagaatt attgaaaaca cacttataaa
    17581 tataaatgaa ataaatatgc agttatctcc tcccaaaatt gagaaaaaac attattgtac
    17641 aaaatgtgct tattatgaat tttgttttgt gaaggaggag taacaatgta taaagactat
    17701 tatattataa gtaatgggga tataaaaagg aaagaaaata cgatttacta ttacaattta
    17761 gaaggtgaga aaaaaatatt accagtagaa caagttgatg atattcatat atatggagaa
    17821 gtagatttaa atacaaaact tataaattac ataagcaaat atggggtgat gcttcatttc
    17881 tacaattact atggatatta ttcaggtaca tattatccaa ggaagaaaaa tgtatcagga
    17941 ttcagtttag tatgtcaatc agcctgctat ttagataaaa atgaaagatt atacttagca
    18001 aaatcctttg tggaaagtgc atcataccat attttaagaa atatgagata ttacaaagtt
    18061 gatgaaagtt taattgatac tataaaggat gaactaaatg atttgaatga tgtcaaagaa
    18121 atatcagaac ttatgggtaa agaaggcaga ataagaaaaa catactataa atcatttaat
    18181 caaatattaa aagatgattt tgaattttat aaaagagaaa aaagacctcc caaagaccca
    18241 gtaaatgctc ttatgtcttt tggaaatagc ataatgtata cgaatgtttt aagtgagata
    18301 tataagacac aacttgaccc tacaataagc tttttacatg aaccatcaac aaagagattt
    18361 tctttaagtt tagatattgc agaaattttt aagccaatta taattgacac aataattttt
    18421 agcctgatta ataataaaag aattaataag aatgattttg aatatcaaga caaaatttgt
    18481 tatttaagtg aaaaaggaaa gaagaaattt ttgcaagaat ttgaaaataa gatgcagaca
    18541 actataaaac atagaaatct aaatagaaaa gtttcatata gaatgtttat aaggttagag
    18601 tgttataaat taattaaaca ttttataaat gatgaaaggt acaaagtttt aaaggtgtgg
    18661 tggtaagtat gtttgttatt gttacttatg atattgttga agcaaggtcg ttaaatagaa
    18721 ttagaaggat acttagaaaa tatttgactt ggacgcaaaa ttctgttttt gaaggcaata
    18781 ttactgaagg aaagttacat aaatgtattt ctgaaataga aaatattatt gataatagcg
    18841 aggattcaat ctatgtttat gagataaaaa atcctaattc aattaaaaag aaatgttatg
    18901 ggattgataa gtattctgat gaaatgttta tataggtttg cagtgagcga tatttatgct
    18961 aaaataggtg ttaacagttg gaatataagg gattgaaggt gtatgataat tgttatcaat
    19021 tgcactactg ctcgctcact gcaaattttg atgtttttat tgaattataa ttgcttgatt
    19081 gaagtgtttt caatgtattc aaatatacct attttggggt tttatattaa ctaagtggta
    19141 tgtaaataat tttttcggat atttaataat ttattttaaa tttagtttta tattaactaa
    19201 gtggtatgta aatctcaaca gtatctacaa gaacttctat atcttctaag ttttatatta
    19261 actaagtggt atgtaaatta atttagggta acttttatat tattttcttc tatcgtttta
    19321 tattaactaa gtggtatgta aatttggttg atatagatat tagtcctatt atttctgctt
    19381 gttttatatt aactaagtgg tatgtaaatt aagttttctt aaaaaagtac ttgctttaaa
    19441 tatgttttat attaactaag tggtatgtaa attttatttt ctctttatta gtcattagtt
    19501 tttgccttcg ttttatatta actaagtggt atgtaaatat gaagaatttg cgaaactaat
    19561 aagaaaagat attgagtttt atattaacta agtggtatgt aaatctttct ttatcaaagt
    19621 aagctaaatt tagttcctcc gttttatatt aactaagtgg tatgtaaatc gtcattactt
    19681 tttattgctt ccattaaatt tgttcgtttt atattaacta agtggtatgt aaatatcgca
    19741 agataggcta aatctatcaa ggagagttga gttttatatt aactaagtgg tatgtaaatg
    19801 actctgccat ccctgtaaca aacagtctta ccgcaagttt tatattaact aagtggtatg
    19861 taaataaata ttttacgatt agttgatgct ataattctat tagttttata ttaactaagt
    19921 ggtatgtaaa ttcttttgat ttagcgttgt ttgtccggaa ttttaaagtt ttatattaac
    19981 taagtggtat gtaaattcgg gctagaatgt attttaaacc ctagttcatt gaagttttat
    20041 attaactaag tggtatgtaa attttctaat atcgctattt aaactcaatg cttcatttgt
    20101 tttatattaa ctaagtggta tgtaaattca gaacaaatta aatatgtttc gttttccatg
    20161 taaggtttta tattaactaa gtggtatgta aatagaactt ttttatatta gaagtacttg
    20221 acatgattgc cgttttatat taactaagtg gtatgtaaat cttgataata tttcagcata
    20281 tgatactgaa gatggtttta tattaactaa gtggtatgta aatttgccaa tgtaacatct
    20341 ttagatatta cagtatcatt gttttatatt aactaagtgg tagataagta gaatattagc
    20401 aatatcaacg agtatttaga aacttatttt atattaataa agcagtatat aaaattgtag
    20461 aatcaacaat agcatatact aaaacatcca ccttatatta actaaatcat atctaaataa
    20521 aataaactta cctataaaca ttataaaatc aatacaaaaa tgaggtgaaa taaaatttat
    20581 gataaagaaa ttaaacaata aagatataaa taaaatcatg gaaatatggg aaaaaagtac
    20641 aatcaaagca catgacttta taagtaaaga atactggcaa aataactata atactgttaa
    20701 aaacgaatat atacctatat cagatacatt tgtatatgat gatggagatg aaataaaagg
    20761 atttataagc ataatagata aaagctttat aggagcttta tttataaagc ccaaatacca
    20821 aaatctaggt atcggaggta aacttttaga ttatgcaact aaaaaatata aaagtctaag
    20881 cttagcagta tataaagata ataaaaaagc agttgtgttt tataataaaa aaggttttaa
    20941 tatagtaaaa gaacaagtaa atgaagattc aggatttaaa gagtacataa tggaatatag
    21001 taaataatat gattacattc tattaaaagg ataattaatt tatttgccat ttgatttttt
    21061 aagagtaatt tttaaaatag actatatgaa gataaagata tttattatag atgtaagtta
    21121 atcagaattg atatagctac aaaagaggaa aataaaattg tatggtgtat ttcagattta
    21181 acaaatttac tatttaaaga taataagttt gatataatat taaagatact ttcacctata
    21241 aactataggg tatttgaaag aattttaaat aattatggaa tgctaataaa ggtttgttaa
    21301 aatttaataa ttattagatt atagaaaatt gccatgaaat tagttcatca agaactagtt
    21361 tcatagcaat tttaattctt ataagtgtat ttatttttac agatttaaaa aacttataca
    21421 gtatctaata atgtaatctt attactttag cctataaata tttaaatctt aaaatgttct
    21481 ctttgagata agtttatttc agagtcacaa tttccacaac agtaagatgt tgagacatct
    21541 ttaaattttt cagtattctc atcaaagttc atttttttct ttgcaataat tcctacctta
    21601 gttaaattag aaccacaatt attacagttt tgaattttgc ataggttata tagtttatca
    21661 agtgtaatat cttctaataa ttcatcacaa ttacaacatc tgatagaaga aacttcacta
    21721 cacatttcaa taggttcagc ttctttagaa gaaaatttat atgttatgtc atttgatact
    21781 ttataacctg tatcaaatat attagaatta caacttggac ataagtactt tggaagatat
    21841 atatcttttt tttcaaaagt atcactacta atattacagt ttaattcatc taaaattggt
    21901 ctttcatcta ggttattacc tttatttaag cttagaagat aattataaat ttttttatct
    21961 tcattctgtt ttgatgtatc taacaaatgc tccattatat tagtgtattt tttgtttttt
    22021 gaaggcatgt ttaaatccag ataattgttt tcaatagcaa agcatactat attgaaatag
    22081 ttatatttaa ctgcaaaaaa taataacatt tcatcaaata ctttatctat tattttgtca
    22141 gaaatactta tccaaaaatc tggtgactct aagtaaactt tcctactaag ttcatctaac
    22201 tcttcatcta tattatttat attattttca ttaactttca tatcatcact tccttttaaa
    22261 tatcttgtgt ataa
//
LOCUS       DAMKMI010000055        22258 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMKMI010000055 DAMKMI010000000
VERSION     DAMKMI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.056"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            764..2434
                     /locus_tag="PC429_001929"
     CDS             764..2434
                     /locus_tag="PC429_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF3847434.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHKMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2556..3290)
                     /gene="pflA"
                     /locus_tag="PC429_001930"
     CDS             complement(2556..3290)
                     /gene="pflA"
                     /locus_tag="PC429_001930"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF3847435.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3450..5681)
                     /gene="pflB"
                     /locus_tag="PC429_001931"
     CDS             complement(3450..5681)
                     /gene="pflB"
                     /locus_tag="PC429_001931"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF3847436.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPGALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5974..7035
                     /locus_tag="PC429_001932"
     CDS             5974..7035
                     /locus_tag="PC429_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF3847437.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNKLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7126..8739
                     /locus_tag="PC429_001933"
     CDS             7126..8739
                     /locus_tag="PC429_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3847438.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9014..10999
                     /locus_tag="PC429_001934"
     CDS             9014..10999
                     /locus_tag="PC429_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF3847439.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKAKSFDEFYKIITKRR"
     gene            11004..11438
                     /locus_tag="PC429_001935"
     CDS             11004..11438
                     /locus_tag="PC429_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF3847440.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11467..12027
                     /gene="srlA"
                     /locus_tag="PC429_001936"
     CDS             11467..12027
                     /gene="srlA"
                     /locus_tag="PC429_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF3847441.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12091..12459
                     /locus_tag="PC429_001937"
     CDS             12091..12459
                     /locus_tag="PC429_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF3847442.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12484..13050
                     /locus_tag="PC429_001938"
     CDS             12484..13050
                     /locus_tag="PC429_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF3847443.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13091..13456
                     /locus_tag="PC429_001939"
     CDS             13091..13456
                     /locus_tag="PC429_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF3847444.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13557..14330
                     /gene="srlD"
                     /locus_tag="PC429_001940"
     CDS             13557..14330
                     /gene="srlD"
                     /locus_tag="PC429_001940"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF3847445.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14679..17033
                     /locus_tag="PC429_001941"
     CDS             14679..17033
                     /locus_tag="PC429_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3847446.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSI
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNCVSDELDMALHKVNCVSDELDMTSNKVNYVSDELDMT
                     SNKVNYVLDELDITSNKTDYISNELYNLSNKSIKDNIPIKMAVITKSNMLNQLPDLVE
                     LERVFSKIEFKVCFDMFITDTATLCDMFIPCTNTLESEDIIYSSMTNPYITYNERAVK
                     PAHKLMDEYYFFMELAKKMGLNDYPFVEKRTYLEKVIEPLKRFDKNLDIEKLKNNYFT
                     IHNPVAWEDKKFETPSGKYELYSESIKNLGISPTPVYISNKYKEIEDKNISFRLLTNH
                     HADTLFSQHFMDKKSIAQAYINQRMAKKVGIEEKDIVILRSKKAKINVQINIDDGVGN
                     YIVKMYVGWWKKHGNPNSLTDTGISDFGGQVTYNESMVEIIRQN"
     gene            17121..17456
                     /gene="spoIIAA"
                     /locus_tag="PC429_001942"
     CDS             17121..17456
                     /gene="spoIIAA"
                     /locus_tag="PC429_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF3847447.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17470..17898
                     /locus_tag="PC429_001943"
     CDS             17470..17898
                     /locus_tag="PC429_001943"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF3847448.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            17921..18685
                     /gene="sigF"
                     /locus_tag="PC429_001944"
     CDS             17921..18685
                     /gene="sigF"
                     /locus_tag="PC429_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF3847449.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            18965..19402
                     /gene="spoVAC"
                     /locus_tag="PC429_001945"
     CDS             18965..19402
                     /gene="spoVAC"
                     /locus_tag="PC429_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF3847450.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19569..20582
                     /gene="spoVAD"
                     /locus_tag="PC429_001946"
     CDS             19569..20582
                     /gene="spoVAD"
                     /locus_tag="PC429_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF3847451.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20596..20958
                     /gene="spoVAE"
                     /locus_tag="PC429_001947"
     CDS             20596..20958
                     /gene="spoVAE"
                     /locus_tag="PC429_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF3847452.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21141..22157
                     /locus_tag="PC429_001948"
     CDS             21141..22157
                     /locus_tag="PC429_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3847453.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
BASE COUNT         8684 a         2503 c         3872 g         7199 t
ORIGIN      
        1 aatcgtattt tttattaatt cttacaaaat caatgaaaat aagtatatcc attttaaagt
       61 acataaattt aaaaatccag aaaaatatac ttaaattatg taagtaaaag aggggttgaa
      121 tcagttgaaa atgtaatatt agcaaatagt acatcaatcc tatataccct gtaagcaact
      181 ctattttcat gtactaaaaa tgtacctatt ttattaactg aaaagaataa attaaatgta
      241 agtacataag aagatagaaa aaattaaaaa caaaaaatat ttatataaat aaatataaaa
      301 aatgatataa tccctattaa aaaatttata gatgatgaag aaatatcaaa atcttatatt
      361 ataggaagag acacttcttt gccaaaatct atagaaaaac aataccaaat ccaaagagaa
      421 taggcggaga aaaaaataaa ataaattcaa gagtaataga aaagttttat agaataaaga
      481 actgataact ctttatataa cgaaaaatag aattttaaga gaaaatgatt taataactga
      541 tttaacatgg tgttttagca ggtaaaaata aatcaccagt aatgattata gaaaaattac
      601 tttatgaatc tcaaaaaact tagttaagag taagagattt gataagataa ctaaagttgg
      661 ggaaatgtgt ttgatgagac aaaaaattag taaaatagta taatctaaat aaggatattt
      721 tgttgattaa actacactaa tagtaatatt gtagggggaa aagatgagag aagaatcgaa
      781 ctctttaagt ttagaccata tagaaatttt ctttgaactt ttatctcaaa gtacagaaga
      841 ttatatattt ttttgggata taaataggaa taaatttaaa atttcttcag caatttttga
      901 tgaatttaac ttaagtaaag agatagaatc tgatttggtc aattgttgga gtaaaattgt
      961 ttatccagat gatgttcaaa tatggaaaga tgatatacaa gaattactac atggaaaaaa
     1021 aggtgagcac aatttagaat ataggttgat taataaatat aaagaaatag tatggatttc
     1081 ttgtagaggt aaagtttatg tgtcggatga cccaaaaact atatttctag tcgggagaat
     1141 aaagaatatt ggtgaaaaaa ataaatttga ttctatcaca ggaacgtgga atagagaaca
     1201 atttgaacat aaaatgaatt atcttataaa agagaaaatt tataaaaatg gtgctatgtt
     1261 tattatggat attgataatt ttaaaaatat aaatgaaaaa tatggacatt cttatggtga
     1321 caaagtgttg cgtgcaattg ctactgaagt actggaatat cttccaaaag atgtaagatt
     1381 gtataggtta gatggagatg aatttgcatt tttttaccct atgtgtacta aagaaactat
     1441 agagaaaata tatgaaaaga tacagatgta tacaaatact cagcatgaaa ttgaatcaag
     1501 taagtactat tgcacagtga ctgcaggcgt tgctatgtat ccagaagatg gagataatta
     1561 tttagattta tttaaacatg ctgatattgc attggatata gcaaaaatta gtggtaaaaa
     1621 tagaattaag tttttttcac aagagctcta tgaaaataaa ctaaaggtaa tttcaatgca
     1681 acaaaaacta agagagtgcg ttgaaaataa ttttaatgac tttgagctgt tttttcaacc
     1741 ccaggtaaat gctgttacaa aagaggtaat aggggcagag gtacttttaa gatggcattc
     1801 gagtacttat ggagaagtat ctcctgttga atttatacca atactagaac agagtaattt
     1861 aattatacca gttgggaagt ggattataaa agaagctgta aaacagtgta aagaatggca
     1921 taaaataaat ccagacttta aaatatctgt aaatgtatcg tatattcagt taaaggaaga
     1981 tttttttaga gattttatag tagaatgttt agtagagtat caattaagac ctgaattttt
     2041 aattttggaa ttaacagaga attgttggat accagatata aatttactaa atgataaatt
     2101 tattagttta aagggtatag gagtatatat tgcaatagat gactttggta caggatattc
     2161 atctttaaat taccttaaag aactgtctgt taatataata aaaattgaaa gaagttttgt
     2221 taagaatatt acatataata gttatgaata tacttttctt gaatatatta ttaaattagc
     2281 tcatattata aatttaaaag tttgtgttga gggaatagag tcttatgaag agtatgatat
     2341 agtaaaaagc ttaggggtag atattattca gggtttttta tttggaagac ctgtaagtgc
     2401 ctctgaattt tataaatttc agctatcaaa gtgatgtata taaaatgaga tatatagtat
     2461 ataaaaatag ggcaaagtgg aagttttgaa gtttattaaa aatttacact ttaccctatt
     2521 ttgttatgtc ttctataaag gattaatata ctacactaac tattatatac tttcactcca
     2581 aatttagaga ttatttcact agctttttgg acatcttcct tagtggcatc aggaacatct
     2641 tttagttcat aatcgatacc taaactttcc catttgtgta ttcccataga atggtatggt
     2701 agcaattcaa atctttctac atttttcaat gtagctacaa attgacctag tttttctaaa
     2761 ttatctatat catcagtaat gccaggaact aaaacatgtc ttatccatac aggaatattt
     2821 cttgattcta agtgtctagc aagttttaat gccttatcca taccaacacc agtcaaatca
     2881 atagattttt cctcaaccat atgttttaaa tcaagtaaaa ccaaatcagt attatctaaa
     2941 actgggtcta tagtttctat atctacaaat ccagatgtat ccaagcaagt gtgtatatca
     3001 ttttcttttg cttttttaaa taattcactt aaaaattctg gttgaagagt ggattcacca
     3061 ccagaggcag ttatacctcc accagaaaac ttcataaaag aagtatactt aagagcatcc
     3121 gttattattt catcaactgt gtattctttt cctgatttaa tatcccatgt atccctgtta
     3181 tgacagtatt tgcatctaag aggacacccc tgaaaaaata atatatatct tattcctggt
     3241 ccatctacag taccaaacgt ttctatagaa tgtatctttc ccttaatcat agagttcacc
     3301 tcacaatata ttttaataac cttatcaata gttataatat ttctctaaaa aaggccatct
     3361 tataccagaa ataattcaaa tataagatag ccttttattt ttatttttta cacagtagta
     3421 atcttagtta aagtataaaa atttaaagac taagccattt taccgtggaa tgttctgttt
     3481 ataacatcta attgttgctc tttagttaat tttatgaagt taactgcata tccagatact
     3541 cttatagtta attgtggata ttcttctgga tgttccatag catcttctaa agtagctcta
     3601 tcaaatacat ttacatttaa gtgatgagca ttttgagcga agtatccatc catcattgca
     3661 gaaagattgt ttattctttc agtcatatct tttcctaaag caccaggtac tatagagaaa
     3721 gtattagata taccatcttg agcatgttca tatggtaatt tagcaactga agataatgaa
     3781 gctaaagcac cattagtatc tctaccatgc attgggttag caccaggagc aaatggagca
     3841 ccagctcttc taccatctgg tgtattacca gttttcttac cataaacaac atttgaagtt
     3901 attgtaagta tagattgagt tggataagaa tttctataag ttttattctt tcttatttta
     3961 ttcataaagc tttcaactaa ctctacagct atactatcaa ctctatcatc attatttccg
     4021 tattttggat aatctccttc tatttcaaaa tcaacagcta ttccttgttc atttctaata
     4081 gttttaacct tagcatgttt tatagcagat aatgagtctg cacaaactga taatccagcc
     4141 ataccacaag ccatagttct aaatatatct ctatcatgta atgccatttc taatgcttca
     4201 taagaatatt tatcatgcat ataatgtatt acatttaaag tatttacata taatgttgct
     4261 aaccaatcag tgaatggttc aaatttactc ataacttcat cataatctaa gtactcagaa
     4321 gttataggtt caaatcttgg tccaacttga acaccagatt tctcatctac accaccattt
     4381 atagcgtata ataaagtttt agctaagtta actctagctc caaagaactg catttgttta
     4441 ccaattctca ttgcagatac acaacaagct ataccatagt catctcccca gtatgctctc
     4501 attaaatcat cattttcata ctgtactgaa cttgtatcta tagatacttt agaacagaaa
     4561 tctttgaatc cttgaggtaa tttagttgac catagaactg ttaagtttgg ttctggagaa
     4621 ggtcctaatg tatatagagt atttaacatt ctaaatgagt ttttagtaac taatgttcta
     4681 ccatctattc ccatacctcc tatacactca gttacccaag ttgggtctcc agagaatagg
     4741 ttattgtaat caggagttct taggaactta accattctta atttcataac aaaatggtcc
     4801 atatactctt gtaattcttc ttctgttacg attccagctt ttaaatctct ttcaaagtat
     4861 atatctaaga aagtagatgt tctacctaaa gacattgcag caccattttg gtctttaaca
     4921 gcacctaaat aaccaaagta taaccattgt acagcttctt ttgagttagt tgctggtttt
     4981 gagatatcaa atccataaga ttcagccatt tttttaagtt catttaaagc tgatatttgc
     5041 tcagttattt cttctcttaa tctgataact tcctcatcca tgcaagaaac ttctaatgat
     5101 tttttttgct catttttatc ttctattaaa gcatcaacac cgtatagagc aactcttctg
     5161 tagtcaccaa ttattctacc tctaccataa gcatctggaa gacctgttat tataccagat
     5221 tttctagcag ctctcatttc tggagtataa acatcaaaaa ctccttggtt gtgtgttttt
     5281 ctgtatttag taaatatatc ttttatttct gggtctaatt catatccaaa agcttcgcat
     5341 gaattttcaa ccatctttat accaccatat ggcatgacag ctctttttaa aggggcatcc
     5401 gtttgtaacc caactattgt ttctaattct ttatctaaat atcctggagc atatgcagct
     5461 attccagaaa cagtttttgt atcaacatct aaagtaccac cattttctct ttctttttta
     5521 aataggacca ttgcttcgtc ccataatttt ttagtatttt cagtggcacc agctaagaaa
     5581 gagtcatttc cctcatatgg agaatagttt aattgtataa actctctaac atttatttct
     5641 ttagtccatc ttcctgtttt aaatccttgc catgcattca ttaagtattt cctccttaga
     5701 tttaaatgat aaataatact attaaaatta atttttatat acttaattta ttttatatat
     5761 ttttttaaaa atatattttc taatataata atagcatatt attcgtatcc tgtatacata
     5821 aaaaataaaa aattgtcgct ttaaagtata aaacagatta tatgttaata atgtggtgaa
     5881 attataccca tatataaata aaataaaaca aataaatagc taattaagga gaaaatattt
     5941 tttatttttt gtttaggata aataaatttt attatggaaa aaagaaaaag catacatctt
     6001 cacaaactga atatttattt aataggggga ggtgatagat tgtttatcac agtggtatta
     6061 tttttagtag gtttcctatt aattactaaa ggcgcagata tctttataaa ttgtactgta
     6121 gagataggta aaaaaacgaa tatatcagaa attatacttg gagctactat agttagtttt
     6181 gcaacaacat tacctgagtt tacagtgtct ctacttgctt ctattgatgg tcatactact
     6241 atgagtttag gaaatgcagt tggttctata atatgtaata cagggttagc attagggttg
     6301 gttgttttta taagtccatt taatgtagat aaaaaaatgt ttttttctaa gtctttacta
     6361 ttaatagtat ccgttatagt tttaatttta ttgagtttag atggagttat aacaagaggt
     6421 gattcattat tacttataat aatattaatc ttttatatgg ttaataatta tagaagtgtc
     6481 gttggaaaat ctgatactaa aaatagaaat attaaaaata acactattaa agacatatct
     6541 actaaaaaag ggaaaaatta tagaggtttt tccgtattag aaattgtaaa aatactatta
     6601 ctttttgcta caggattgat aatgatgata ataggttcac aaatccttat agagagtgga
     6661 gtaataatag ctagtttttt aaatatacct caaggtatag taagtttaac tataatagca
     6721 cttggaactt ctctgccaga gatagtttcg tcgattactg ctataagaaa aaatcatcat
     6781 gaaatatcag taggaaatat tttgggtgca aacatattga atatagtatc agtaatagct
     6841 gtatcagcaa taccaaataa cataccaata ctatctcaaa atagacaatt ggatatacca
     6901 ttcatgatat tattattatt aatagtaata ataccaacac ttaaatctaa taaacttagt
     6961 agaatacaag gtatattgat gttattcaca tattttctgt acatatcaat tttgtatttc
     7021 atgtatatta tttaactata ctgtttaaaa tattaattgc agaatatcct aaaaaagtat
     7081 ataatggata ggttaagatt aattgataac ggagaggaat aatatatgaa tataacaaag
     7141 tttgaagatt taccaataag tgagggaata aaaaaagcta tagctgaaat ggggtttgaa
     7201 gaaccatctc ctatacaagc acagtctata cctgcgattt tgtcaggtaa agacgttata
     7261 ggacaagctc aaactggtac gggaaaaaca gctgctttta gtatccctat actagaaaca
     7321 atagacccaa ataatagaag tttacaagct gtagtgcttt gtccaacaag agaactggca
     7381 atacaagtat caacagaaat aagaaagtta gctaagtatt cgcacggaat aaagacatta
     7441 cctatatatg gtggtcagcc aatagataga caaataaaat ctctaaagag tggtgtgcaa
     7501 gttgtaatag gtacaccagg acgtacaata gaccatatca atcgtaaaac attaaagatg
     7561 gataatgtaa aaatgattat attagatgaa gcagatgaaa tgctagatat gggatttaga
     7621 gaagatatag aaatgatttt aagtaagatt ccagaagaaa gacaaacaac tttcttttca
     7681 gctactatgc caagaggaat tttggaatta actaagagat atcaaaaaga cccagagcat
     7741 ataaaagttg ttagaaaaga acttacagtt tcaaatacaa agcaatacta tatagaaact
     7801 aggtcatcaa ataaattaga agtattatgt agattagtag atgtatatga ccctaaacta
     7861 tctgttgttt tctgtaatac taagagaaaa gcagacgaat tggtaggaga tttacaagca
     7921 agaggatatt ttgcagatgc attacatggt gacttaaaac agactcaaag agatatagta
     7981 atggataagt ttagaaatgg tacaatagat atacttgttg ctactgatgt tgcagctaga
     8041 ggtatagatg ttgatgatgt tgaatgtgta ttcaactatg acttaccaca agatgaagaa
     8101 tattacgttc atagaattgg tagaactggt cgtgctggta gagaaggtat gtcatttact
     8161 tttgtatttg gaaaagaaat gagaaagatg aaagatatag aaagatatac taaatctaaa
     8221 ctaataaagc ataatatacc aacaataact gatgttgaag agaaaaaagt aggtactttc
     8281 tttgcacaag ttaaacaaac tatagaagaa ggtcacttaa ctaaacaatt acaatggtta
     8341 gaaggtttct gtaatgatga agattatgca atggttgata tagctgctgc acttgtaaaa
     8401 ctatctctag gtgaagaaat gaaagaagaa ataatagaag aaaaacctag aagagagcgt
     8461 ggagatagaa aaggtggtac tggagctaaa gatggtatga ttagattatt tatcaatatt
     8521 ggtagaaatc aaagagtaca agcaaaagat atagttggtg ccatagctgg agaagttgga
     8581 ataccaggta aagtagtagg tactatagat atatatgata agtatacttt tgttgaaata
     8641 cctaaaaaag atgcaaaaac agtaattgaa aagatgaaag atattaagat aaaaggtaat
     8701 aagattaata tagaaaaagc aaataagaag aaaaaataac gtatagtata caaaactata
     8761 attagaaaat atataaaata atatattgga tatttaaaaa gcctgatgtg ttttcaaaac
     8821 agatagtaga taatctgtaa gagttaacag cataaggctt ttcttgtttt atatgatgtt
     8881 ataattttcc agattgttgt gaaaaatgta tattagttcc tttttttcaa atctgatttg
     8941 aaaaagagaa tattaattat ctatatagtt tagattataa taaaaaatgt aagataacaa
     9001 aggggagaaa aagatgtcaa tgacttatat ggaccctagg gtttttttga ttttagaaat
     9061 ggtgttagaa gaagacacaa atactctaga acaattttca aatgttttgg gtgttagtac
     9121 taggactatc agaaactata taaaccagat taatcgagaa ttaaaaggtg gtattgctaa
     9181 aatagttaaa aatgaaaaag gtacatatag tataagaatt gaggatgaaa gtaaattaat
     9241 tgagattgta aattttaata gaggaaagct atcaaatttt ataaatttaa attcacctga
     9301 tgaaagaata aattatgttc ttgatatttt aactatgacc aacaatccaa ttaccataga
     9361 tgatttggct gaagagatat gtataggaag aacaacatta ataaaggatt taaaaaaagc
     9421 agacagaatt ttagctgaat atgatttaga attaaaaagg aagccaaata taggtatgat
     9481 tttagttggt gaagaaatgg atataagatt attaatcctt gatagattgt atgaaaatta
     9541 tattgatgtc ttggaaaacg ttaatcagat aaatttagtt aaagattatg atattgaatg
     9601 tcttagagat gaattaaaaa agttatttaa aaaagaagaa ctctacataa cagaacaggt
     9661 cttgagagat gttgaaagat atataatcat gtctgtctta agaaatctaa atgggtacaa
     9721 atttgaaaag atagataaga gatttgaagt gataagatgt tcagatgaat atactttagg
     9781 attaaagctc aaagctttat tggaaaagat atttaatata gttttaaatg aaaaagaaac
     9841 catatttttg acaatgccat taataggtag aaaagctcct agtacaaaat tagcacttag
     9901 cagtataaag ataaatgata gcgtaaagga agttgtagat gaagtaacta gttttttatt
     9961 agagacctca gggattgatt ttaaagaaga taaaaagctt atagaaaatt tggaatatca
    10021 cttgtatttt gcacttaata gaatgagatt caacataagg gtaaaaaatc ctcttttaca
    10081 agaaataaag gaaagatata catttccata tagtgttgca aagatagctg caatgataat
    10141 tgaggaaaag tttgacttga aaatatgtga ccacgaaata ggatatatag cattacattt
    10201 tggaagttat tttgaaaaaa attcaagaaa agttctaaat gtaaataggg tagctgttgt
    10261 ttgtggaaca ggtcttggga ctgcacaact tattagaata aagataggaa aaatacttgg
    10321 agaaagtgta gaagtaaata ctttttctga tatagaagct aaagctaatt tagatttatt
    10381 aaagtattat gatttagtat ttacaacagt agatttcaaa acagacttag atgttccaat
    10441 ttttagagta aatgcgttat ttaatgaaga aagtataaag aaagagatag aaaatgcaat
    10501 acttttgaaa aatagtataa atcttaagtc tataaaaaag attgaaaaaa atatcccttt
    10561 tataggtaaa ttaattgaag aggataagtt ctttatattg aataagagta cgtttatgga
    10621 aaatttagaa gatatgttag aaaaattagt tcataaagga attattgata tagagttcaa
    10681 aaaaaaggta ttaaaaagag aaaagaaatc ttcaacagca ttagataatt atatagcatt
    10741 gcctcactca gttaatacta atggggaaaa tctttatctg gcatttggaa tattagaaaa
    10801 accagtgatt tgggatacaa aggaaattag aataataata ttgatgataa ttccagataa
    10861 gaatgtagat agtacagacc tgataataaa aagttatgaa gaagtacttg aagtagggag
    10921 agataaaaag atggtagaag aacttgctaa ggcaaaatca tttgatgaat tttataaaat
    10981 aattaccaag aggaggtaat aatatggacc cagtaatttt tattatagca tcacttatta
    11041 cagtgtattt attaaatatt tttcttggat acttacagct taaagatttt aataaaaatt
    11101 atattgaact aaaaagaaaa ggaagagtgg caattggcag aaaaaaagga agaatttcat
    11161 cgggtacaat tgtgttgata cttataaatg atgatggtgt gattgtagag actagaaaaa
    11221 tgcaaggtgt tacagtattg gcaagagtaa agtcatttga aggtttggta ggaaagagct
    11281 tagagtatat agaaaaatca gatttaactg tttacaataa actacttaaa aaagcaatac
    11341 tagatgcagt caaacagtac ataacatttg agaaaaataa atgtgaagat acaaataatc
    11401 tgagtactga tgaagagtgt aggatggaag tagtttaaaa aaatatattt ataaggagga
    11461 gttaatatga cgcaattttt tcaagtatta gcaagtggtg cagatagttt tatgaattta
    11521 tttagggcag gtgggcaaca atttgtaggt tttgtaacag atatagtacc acttttagta
    11581 gcattattag ttacaatgaa tgcaattatc aactttattg gtacagatag agtcgagaga
    11641 cttgcaaaaa aatgttctgc aaatatattt actagatatt tgatattacc agtccttggt
    11701 acatttgtat ttgcaaaccc aatgacacta tcattaggaa gattcttgcc agaaaagttt
    11761 aaacctagtt actttgcagc agcaagcttt tcatgccata caatgaatgg attattccca
    11821 catatcaatc caggtgaact atttatatat ctaggaattg caaatggtat aacaactttg
    11881 ggattctcaa cagcagattt agcagttaga tatttgttag tgggtatagt tgcaaacttt
    11941 attaaaggtg tagttactga ctatacaaca aaatttgtag agaaacaaca aggaatagca
    12001 ttaaattcag atataaagat tggataatta ctttaaggta taaataaatt tttcaaataa
    12061 aaagatagtg tatagaggag gataaattat atggcattta acaaagttaa aatagtaaaa
    12121 ggttctggag gatggggtgg accattgata atagaaccaa cagaaaagaa gaataaggta
    12181 gtatatgtga ctgggggagc aaagccagaa actgcagtaa aaatagctga acttacagga
    12241 tgtgagttaa tagatggatt ttcacagggt gttaaagatg atgaaatcgc atgtgtaata
    12301 ataaattgtg gtggaacatt gagatgtggt atatatcctc aaaagaaaat accgactata
    12361 aatataatga aaacaggaaa aagtggacca ttagcaatgt ttataaaaga agatatatat
    12421 gtttcagggg taaaaccaac aaatgtagag cagttataat tgaggtagta ggagggaaga
    12481 aatatggctt atgatactaa aaagaagata agtgagcaga gtcaaggaat tgttgcaaaa
    12541 ataggtatag cactaggaaa tgtgacaagt actctttttc aagctggtag ggatactata
    12601 gatacattaa tacatactat actgcctttt atggctttcg ttgcagtcct aataggtata
    12661 ataaatcaat caggtatagg aaattgggta gccaatttac ttactccatt aatcggaaat
    12721 gttggaggtt tagttgtctt atcaataata tgttcatttc cgttgttgtc tccgtttctt
    12781 ggaccaggag ctgttatagc tcagatagtg ggggtattgg ttggtacaga aataggaaaa
    12841 ggaaatattc caccaagttt agcattacca gcattgtttg caataaactc acaagcagct
    12901 tgtgacttta taccagtagg tcttggtctt gcagaagctg aacctgatac agttcaagta
    12961 ggagttccgt cagttttata ttctagattt ttaacaggtg caccaactgt tctattagca
    13021 tggttagcaa gctttgggtt atataaatag aagaatactt tataaataat tagagagagg
    13081 ttcagttgat atggaattaa tatacagtac tcaaataaat aaaataggag ataatgcaaa
    13141 tgaattttta caacataata tgtttgtaac tttcaaagat aatgctcctc aagagttaga
    13201 aaattactgt tatatacatt ctgaaaataa tttagtaaaa gaaatactta cggatgatgt
    13261 gctatcaata aataatgttg attacaaaat tacttctgtt ggtgagttag taaaccaaaa
    13321 tctttcagaa ttaggtcata taacatttaa gtttacaggt gaaaaagatg gagcaattgg
    13381 aggaacctta tatcttgaag aaaaagaaat agcacctatt ggcgtaggta ctatactaaa
    13441 aataattaga aaataaaatt taaaacgaat ctaatcatat tataagtaaa ttatagataa
    13501 aagtcaattt aactaatcaa gttaactttt aatgtgataa aaaaggggaa atgaaaatga
    13561 gaaaagtggc tttagtcgtt ggaggaggaa gaagtttagg tgaatattta tctaaacatt
    13621 tagctaaagt aggttatgat gttgctattg cagatttaaa tgaagacaac gcaaaaaaag
    13681 tagctgaaga aataaaaact gaatttggtt gtaaatctgt aggatatagt tgtaatgcca
    13741 tagatgaaag acaggttata agtactgtca aagctattga aaatgatttt gatagaatag
    13801 atttattagt gtacaatgca ggagtggctg taagtaaaaa aataactgat tttggataca
    13861 aagattttaa atttattgta gacgtaaatt taaatgggta ctttctattt gcaagagaag
    13921 tatctaaagt catgatagca aaaggtattg agggtagaat aatacagata aattcaaaat
    13981 caggtagagt gggaagcaag cataattcag gatattcagc aggtaaattt gctggagtag
    14041 gacttactca aagtttagcg ctagatttgg cagaacataa gataactgta aatgcactga
    14101 tgctcggtaa tttacttgat tctgatatgt ttgaaagttt aattcctcaa tatgcagtaa
    14161 aattgggaat acctgaatca gaagtcaagc aagtttatat agataaagta cctttaaaaa
    14221 gagggtgtag atttatagat gttgcaaata tgcttacatt ttatgcttca aaggaagctg
    14281 aatactgcac tggtcaatct ataaatatta ctggtggaca agttatgtaa ggtttttatg
    14341 gagtgtctaa agatgaactc tttagttcat gaggcacttt tttgtataaa attaaatata
    14401 ttttcagcat ttaaacaatg aaaaagaggt ttatctttat tttgagatag cctcattttt
    14461 gtaattaaaa aacatagtgt ttaagtaaac atataagatt gttagttttt taaaagagaa
    14521 ttttttaata atattatatt tatgaagtat ttttaaactt aatttgataa aatatagcta
    14581 tgttttatat actgtagttt tgaacataga aagtttaata aattatagta tatatgttaa
    14641 tttttaataa ggtaagaaag tagaaagaag gatattttat ggatataata aaaaaattaa
    14701 gtcatgggtg tacattagat tgtcatgact gttgtaaatt caatgtttat actaaaggaa
    14761 ataatgtagt aaaaattgaa ggagataaaa atcatcctta tacaaaaggt tttatatgca
    14821 aaaaagggat ggcccattta gatagattaa atcataaaga tagaataaag actcctatgc
    14881 ttaaagttga tggagtatgg aaagagattt cttttgataa agctatagaa ataatggcag
    14941 aaaagcttac atactataaa gaaaagtata catcaaagtc tatcatgcat tatgaccaat
    15001 atggaagtgg gtctgtgcta aaatacatag gagatatatt ttttaatttt tatggaggtg
    15061 ttagcagaca taaggggggt ccctgttgga gtgcagggat gcatgctcaa aaatatgatt
    15121 ttggagtagc taagagtcat gctatagaag atatgttaaa tagtaaaagt atatttgttt
    15181 ggggaaaaaa tccagcatat actacaattc atactatgca aataattaaa aaagctaaag
    15241 aaaaagggat aaagatagta gtgattgacc ccatatatac aaaaacagct caaatagcag
    15301 ataagtatgt acaagtaaat cctggaacag atggagcttt agctatagct atggcaaaaa
    15361 ttatagtgga agataaatta tatgatgaag aatatataaa ctcgtatgtt attggttttg
    15421 aagaatataa aaaatattta tcttcattgg aattaagttt tttaattgat gaatgtggtg
    15481 ttaaagagaa tgatataaga gagttagtgg atttatatac taacaaatat tcaagtataa
    15541 atgttggata tgggcttcaa aaatataaaa atggtggaaa cacaataaga gcaatagatg
    15601 ctttaggtgc tataactggt caaataggat ttagtggtgg aggggtaaac tatgctaata
    15661 aagtttatcc gagtgttata aattctgacc cttacaatag ccaatcatat ggtgaagata
    15721 gggagtttta tgtaagtaat atcagtaaat ttatagaaga atctttaaaa aatacatcaa
    15781 ataaagtaaa ttatgcatca gatgaacttg atatggcatt acataaagta aattacatat
    15841 cagatgaact tgatacaaca tcaaataaag taaattatgt atcagatgaa cttgatatgg
    15901 cattacataa agtaaattgt gtatcagatg aacttgatat ggcattacat aaagtaaatt
    15961 gtgtatcaga tgaacttgat atgacatcaa ataaagtaaa ttatgtatca gatgaacttg
    16021 atatgacatc aaataaagta aattatgtat tagatgaact tgatataaca tcaaataaaa
    16081 cagattatat atcaaatgaa ttgtataatt tatctaataa gtcaatcaaa gataatattc
    16141 ctataaagat ggctgtgata actaaaagta atatgctgaa tcaactgcca gatttagtag
    16201 aattggagag ggtgttttca aaaattgaat ttaaagtatg ttttgatatg tttataactg
    16261 atacagctac tttatgtgac atgtttatac cgtgtacaaa tacactggag agtgaagata
    16321 taatttatag ctcaatgacc aatccatata taacctataa tgaaagagct gtaaagccag
    16381 cacataaatt gatggatgaa tattacttct ttatggagct agctaaaaag atgggattaa
    16441 atgattatcc ttttgtagaa aagagaactt atttagaaaa agtgattgaa ccacttaagc
    16501 gttttgacaa aaatctggat atagaaaaac ttaaaaacaa ttactttact atacataacc
    16561 ctgttgcttg ggaagataag aagtttgaga ctccatctgg aaaatacgag ctttattcag
    16621 aaagtataaa aaacttggga ataagtccaa cacctgtata tataagcaat aaatataaag
    16681 aaatagaaga taaaaatatt tcttttagat tattaactaa tcatcatgca gatactttat
    16741 ttagtcagca ttttatggac aaaaaatcta tagctcaagc atatataaat cagagaatgg
    16801 ctaaaaaagt aggtattgag gagaaagata ttgtgatatt gaggtcaaaa aaagcaaaaa
    16861 taaatgtaca aataaatata gatgatggag taggtaatta tatagtaaaa atgtatgttg
    16921 gttggtggaa gaaacatgga aatcctaata gtttaacaga cactggtata tcagattttg
    16981 ggggtcaggt aacatataat gaaagtatgg tagaaataat acgacaaaac tagttaaatt
    17041 tcattgaagg aataaaaata taattataga attgattaaa agttactatt tttacaaaac
    17101 tattgaagga gggatttttg atggtaaatt attccttaga acataagaat ctatacatag
    17161 aatttatgtg ctcagaacta gaccaccatg tagccaatga aataagggaa gaaatagata
    17221 acttattaag tgtaaatcaa gtaaaaaatg ttgtatttaa ttttgaaaat attaatttta
    17281 tggattcttc aggtataggc gtcatcatag gtagatataa aaagatttct aatgaagggg
    17341 gacgagtttc agtaattaat ataagttcac gagttaagaa gatttttgat ttatctgggt
    17401 taaataaaat tattggcatt tatgatacgt atgaagaagc tctaagttct ttgtagggag
    17461 gagtatgtga tgaataatat tatggaagtt aaattttcgg caatatctga aaatgaaagt
    17521 ttagcaagag ttatagtagc atcctttgca gctaagttag acccaacagt agatgagtta
    17581 gtagatataa aaactgctgt ctctgaggct gttacgaatg ctataataca tggatatgaa
    17641 gaagacagtt ctaagtttgt tttcttaaga tgtgagatag aaggaaatac tataaagatt
    17701 atagttgaag atgaagggta tggaatagaa aatgtggaga aggcaatgga accactttat
    17761 acatcaaaac cagaacttga tagatcagga atgggattca cagtaatgaa aagctttatg
    17821 gacgatgtag aggtaagttc tgtaaaagat aatgggacta gaattgaaat gactaaaaaa
    17881 ataaatgtgc ctaaataaag tccaaaagtg aggttttatt atggaagtaa ctgttgccag
    17941 agaagaaaaa aaacctcttc ttagtcatga agagactcta gaacttattg aaaaagttca
    18001 aaatggagat gaagaggcaa aagaaatatt aatatcaagc aatttaggtc tagttaggag
    18061 cgttgtatct agatttttaa atataggata tgatagagaa gatttatttc agttaggttc
    18121 tataggctta ataaaatcaa tatataaatt tgacccaaaa tttaatgtaa aattctctac
    18181 ttatgcagta ccaatgattt taggtgaaat aaagagatac ttaagagatg atggtatgat
    18241 aaaagtatca aggtcgttaa agcaaatagc tgtaaaggca aagatggaaa gtgaagcttt
    18301 aactaaaaaa ttaggtagag agccgagtat agaagaatta gctaaagcaa tagatgtaga
    18361 aaaagaagat ttagtaatgg ctatggaagc taatttcaat gttgagtatt tgcaaggagt
    18421 tattcatgaa gaggaaggtt ctccaatatg ccttattgat aaaataagca tgaaaggaga
    18481 aagtgaagaa gaaaaagtag tggataacat attgttaaaa gacatacttg gaagactgga
    18541 taagagagag cgtcaaataa tagttctcag atactttgaa gacatgactc aaagtgaaat
    18601 aggagagatg ctcaatatat cacaagttca agtatctagg atagagaaga aagtattgtc
    18661 taagttaaaa gaatatatat cgtgataata cttatgatat gtagaataac aaaatataat
    18721 aaatatatta ctaaaataaa atgtaataac tttttatgtt aagtttttaa attattaaaa
    18781 gatattaaaa taataaaaag cattaaaaaa gtaaaaatat aaaaacaagg tgtctttatg
    18841 gaaaaataga ttttttcttt attaggcatc ttttttattt tttgaccaaa ctttagatag
    18901 tcaaaataaa taataggttt atttttttaa aattaacaaa taatacttta gagaggtgtt
    18961 tagtatggat aaaaattata aaaaatatgt agaccaaata agcccaaaac ctacatattt
    19021 aaaaaactat acacttgcat ttattgtagg tggaattatt tgtatgatag ggcaagcaat
    19081 aaatgatttg tatatgaaag ttggaggtct tgataaacta ggtgctagtt ctgcaacatc
    19141 aataacatta atatttatag gggcattttt aactggatta ggagtttacg acctaatagg
    19201 aaaaagagct ggagctggtt ctataattcc aataacaggt tttgctaact caatagtatc
    19261 tccagcaatg gagtataaaa gagaaggcta tgtattaggt gtaggtgcaa acttatttaa
    19321 aattgcagga ccagttttag tttatggtat aggttcttct attttatgtg gaattattta
    19381 ttatattttt aagatgtttt agatgatttt tatttaattt aaattttatt aattatgaag
    19441 aaggtataaa atctgaaaat agctactaaa gtaactaatt gaaaaataat tgtgcagatt
    19501 taattaatat ttttaattaa tattaaaaat ttgaatagta aaatgcaaaa ttaaaaaggt
    19561 gtgaaaatat gaaaaataaa agaattggaa aaagaacagt caagttagaa aataaaccaa
    19621 ccataatatc aactggaact atagtaggtc ctaaggaagg tgaaggacca ctaaaggact
    19681 attttgatat gattatgact gacgatttat atggtgaaaa gacttgggaa ttagctgaaa
    19741 gtaaaatggt agagactgca tctcaacaag ctatccaaaa ggcaggtaaa aaattgtcag
    19801 atgtaaatta tatgctaggt ggcgatttaa taaatcaaat agttccagca tcatttgcag
    19861 caagagaact ggctattcca tttttaggta tatatggagc ttgttctaca atggcagaag
    19921 gtctttgtat aggttctatg cttatagatg gtggttttgc tgatttagta ctgtcaggta
    19981 catcaagtca ctattgtaca gcagaaagac aatttagatt cccacttgaa cttggaaatc
    20041 aaaaaccaat gacagcccag tggacagtta caggagcagg aagtgtatta ttagctccta
    20101 atggagacgg tccaaaagta aagtatgtta ctgttggtaa agtaatagat gagggtattg
    20161 atgatggtaa taatatggga ccagctatgg ctccagcagc aatagatact atctactctt
    20221 actttgaaga tactaatgat gacccaaata gctttgatat aattgctact ggagatttag
    20281 gtactcttgg aaaacagata gcagaagact ttctaaaaga aaaaggtgta gatatatcaa
    20341 aagtttacac tgactgtggt atagagatgt ttaaccttaa agaacaagat gtccactgtg
    20401 gtggaagtgg atgtgggtgt tcagctacag tgtttgcagg atatatatat gataagttga
    20461 gaaaaaaaga atttaacaaa gtaatgcttg tatcaacagg agcactactt tcaccgacaa
    20521 gtacactaca aaagcagaca ataccatgtg ttgcacatgc agtagtaata gtaaatgagt
    20581 aggaagtgat ataatatgtt aatggactat gtaagagtat ttattgtagg tggaattata
    20641 tgtttaatag cccaagtaat gatggattat ttcaaaatgc aaactccata tgttatggta
    20701 acgtatgtaa caactggtgt agttttaact tttctaggac tttatgagcc attagttaag
    20761 tttgcagcag caggtgcaac agttcctata ataggatttg gatattcact tgctacagga
    20821 gtaataaaat caattcaaag tgatggtttt ttaggtattt ttttaggagg tacaactgct
    20881 acagctggtg gaatagcagc agctatattc tttggatata ttatgtcagt agtatttact
    20941 ccaaaagcaa aaccataaat ctttattaaa agaataataa gtttttgaaa taataacttt
    21001 ttataaagca ggtggatatc ttttagattc cacctatttt ttatataaag aagcgtttac
    21061 ataattgaag ttgacaatta tttacaacat agtgtagtat tatattatca aaataaaatg
    21121 aagtgaagta ggggataaag atggtaacta ttaaagatat tgcaaggaat ctaggtatca
    21181 gttattcaac tgtttctagg tgcttaaaca ataatcctaa tgtatctgaa aaaacaaaaa
    21241 agaaagtggt agaagaggca aatagactag ggttccattt taatgtaaat gcaagaaatt
    21301 tagcaaaaaa agaaactaac aggatagggg taatcttttc aaataatttt aatcatcaag
    21361 atactagaaa gttttttagt gatataatgg atagttctat aaattctatt gagactaata
    21421 aatacgattt tataatacaa cctaataata acattagcgg tgatagtaat gtatataaaa
    21481 tggtaaatgg acaaatggtt gacggattag taattgtgtc tcaatcaata aaaaaagaag
    21541 agtatgattt tttaaaagac aataattttc cacatgtatt tatatatttt aaaccatctt
    21601 ttataggaga agtagacaat ttcttttggg acgataacgt gtatggaggt tatatagcta
    21661 ccaagcattt aattgaccat ggtcataaag atataataac agtaacttca gatgataaga
    21721 gttcaaaaat gcatgaagat agaaccaagg gatatttgaa tgctatggaa gaggcaaatt
    21781 taaagaccga agtcattaaa tctaaaatgg attttgaatc tcaagttgaa tttttagaaa
    21841 aatacattga taaaataaaa aaagcttcag ctatatttgt acaacaagat gtaccagcga
    21901 tttctataat acaagagtta aaaactactt atggtataaa tgtaccagaa gatatttcca
    21961 taatagggta taataatatt gaattaatat catattttag gtcgcattta tcaacaatag
    22021 atgacccaag agagcaagtt ataaaaaatg gagtagatag tttagtaaat ataataaata
    22081 aaaagagtac agaacacatt ccaagaaaat tatatccaag gttaataata cgaaatagcg
    22141 ttaaaagaat aaagtagtaa gcttttaagt atcaaacgtt tattaaaaat attatagatt
    22201 atgtgaagtt ttttaacaaa taatataaat aaaaattaaa aggagtaggt gtatataa
//
LOCUS       DAMKMI010000056        22238 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMKMI010000056 DAMKMI010000000
VERSION     DAMKMI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.057"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            143..733
                     /locus_tag="PC429_001949"
     CDS             143..733
                     /locus_tag="PC429_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3847454.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            847..1692
                     /locus_tag="PC429_001950"
     CDS             847..1692
                     /locus_tag="PC429_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847455.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            1689..2372
                     /locus_tag="PC429_001951"
     CDS             1689..2372
                     /locus_tag="PC429_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847456.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            2369..3085
                     /locus_tag="PC429_001952"
     CDS             2369..3085
                     /locus_tag="PC429_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847457.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            3182..3445
                     /locus_tag="PC429_001953"
     CDS             3182..3445
                     /locus_tag="PC429_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847458.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            3417..3872
                     /gene="def"
                     /locus_tag="PC429_001954"
     CDS             3417..3872
                     /gene="def"
                     /locus_tag="PC429_001954"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF3847459.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            3924..4385
                     /locus_tag="PC429_001955"
     CDS             3924..4385
                     /locus_tag="PC429_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF3847460.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            4435..5337
                     /locus_tag="PC429_001956"
     CDS             4435..5337
                     /locus_tag="PC429_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3847461.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            5707..6519
                     /locus_tag="PC429_001957"
     CDS             5707..6519
                     /locus_tag="PC429_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3847462.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            7567..8328
                     /locus_tag="PC429_001958"
     CDS             7567..8328
                     /locus_tag="PC429_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF3847463.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            8393..9175
                     /locus_tag="PC429_001959"
     CDS             8393..9175
                     /locus_tag="PC429_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF3847464.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            9442..9861
                     /locus_tag="PC429_001960"
     CDS             9442..9861
                     /locus_tag="PC429_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF3847465.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(9964..10230)
                     /locus_tag="PC429_001961"
     CDS             complement(9964..10230)
                     /locus_tag="PC429_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF3847466.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            10457..11158
                     /locus_tag="PC429_001962"
     CDS             10457..11158
                     /locus_tag="PC429_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847467.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            11240..11848
                     /locus_tag="PC429_001963"
     CDS             11240..11848
                     /locus_tag="PC429_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF3847468.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            12172..12636
                     /locus_tag="PC429_001964"
     CDS             12172..12636
                     /locus_tag="PC429_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HDF3847469.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            13273..14289
                     /gene="gap"
                     /locus_tag="PC429_001965"
     CDS             13273..14289
                     /gene="gap"
                     /locus_tag="PC429_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF3847470.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            14684..15853
                     /locus_tag="PC429_001966"
     CDS             14684..15853
                     /locus_tag="PC429_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847471.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            16233..20486
                     /locus_tag="PC429_001967"
     CDS             16233..20486
                     /locus_tag="PC429_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF3847472.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            20635..21129
                     /locus_tag="PC429_001968"
     CDS             20635..21129
                     /locus_tag="PC429_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF3847473.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            21245..21622
                     /locus_tag="PC429_001969"
     CDS             21245..21622
                     /locus_tag="PC429_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF3847474.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            21828..>22238
                     /locus_tag="PC429_001970"
     CDS             21828..>22238
                     /locus_tag="PC429_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF3847475.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKD"
BASE COUNT         8984 a         2150 c         3782 g         7322 t
ORIGIN      
        1 taataaaaaa tgctatataa aattcagaat tttcgaaagt ttttaagctt atttttaaat
       61 atactaataa tactattgac attagataga aaaagtatta tattgactat ataaattatt
      121 ttagtcaaac tggagggaaa agatgcctaa atcatattct gataaagaaa gagagtatat
      181 aataaaaaga cttaaagaag aagcaagatt gtgtatggag caatatggta ttagaaaaac
      241 tactgttgat gagcttgtaa aacgggtaaa aataccaaaa ggaacttttt atctattttt
      301 tcaatcaaaa gaattactct tttttgaggt attaagagat atacatgact ctatacaaaa
      361 agaaatctta tatgagataa atagagtaga tgaaagtatt acatgtgaac aattgacaga
      421 tatatttatg aaattctata ggatggttga tagtacttca attttaaatc tcatgataaa
      481 tggagaattt gagatattgg tgagaaagtt accagattct ataattgaag aatactttag
      541 acatgatgac tttgaaattg gagagattat atcttgcata ccaaatgcaa aaaataaaga
      601 catagaaagc tttagtggag catttagagc agtttttctt accatgctat ataagcgcga
      661 ggttggaagt aattgttttg aagatgcttt aagattaatg ataaacggat tagttataca
      721 acttatggaa tagaatttaa attttgtttg ctaataactt ttattaaaaa ttattaacaa
      781 acaaaaaaat tatattttat tgaccatata gtcaaaatag tcaaaaattt gaaagggtga
      841 tattgaatga ttaaagttga tgatttatca tttagttata cagatagaga ttttttacaa
      901 aatattaact ttgaagtagg aaaaggggaa atactaggat ttttaggacc atcaggtgca
      961 ggaaaatcta cattacaaaa gattctaatt ggaatgataa ctaattatgg aggaagtgtc
     1021 attgtaaatg gagtagagag taaaagacac tcaaataaat tttatgaaaa tataggagtt
     1081 gattttgaat ttcctagttt atatgaaaag ttgactgcaa tagaaaattt aaaatatttt
     1141 gggtctctat attctaaaaa actactatct attgatgagt tattaaaatc agtaggacta
     1201 gaaaatgaat caaataaaag ggtatctgag tattcaaaag gtatgaaatc tcgattaaat
     1261 tttataaaag ctttattaca taatccagat attttatttt tagatgaacc aacaagtggt
     1321 cttgacccat caaatagcaa ggttatgaaa gatataattt tatcagaaaa atcgaaaggt
     1381 aagactatta ttttaacaac tcataacatg ttagatgcaa cagagctttg tgacagagta
     1441 gcgttcattg taaatggtaa aatatctgct ttagatacac cacataatct tattatgtca
     1501 aaaggagcta ttaaagttag atatacttac tttgataatg gagaaaaaac ttctgaatgt
     1561 tttctgaaca atactgctaa tgataaaaat ttgaatatgc ttattgagaa aaataaactt
     1621 ttatcaatac atagtagtga accaacatta aatgatattt ttattgagat aacagggagg
     1681 aatttacaat gaggctaaaa aatcttattt taggagatat aaagtttcaa tttaagtatg
     1741 gtttttattt tttatatctt tttttaagta ttatatatat ttgtattatc aatgtatttc
     1801 caacttttat gagagaaaaa atagctataa ttatgattta ttctgaccca gcagcaatgg
     1861 gattgttttt tatgggagct attgtacttc ttgagaaaag tcaaagggtt ttaaattcct
     1921 tagctgtatc tccagtaaaa gtatctgagt atattttatc aaaggtaatt tcattaggag
     1981 taatatctag tattgtagct atgtttattg ctattacatt aaatttagat aatattataa
     2041 tatcaactat aggtaccttt ttttcttcta ttatattttc tttgcttgga ttgataattg
     2101 caagtaaagc atctagtttg aatcagttta ttgttttaag tattcctatt gagattatat
     2161 gctttattcc accaatactg aatgtacttc ttgacacaaa atcatatgca aatttgtacc
     2221 cttttaatat atgtatttct cttattagtg gagataaaaa ttttataatg attaatattt
     2281 taattttaat atcaattata ataataattt attttataac ttattatttt atttgtagca
     2341 gttggaaaaa ggtaggtgga gtaaaactat gataaagatt ataaatgcgt ttaaacaaga
     2401 gtttgaacaa attaagagag atgctatgct ttttatagta tgtgtttctc caatattatg
     2461 tggagtattt ataaaatttg gaataccatt aatacagaat atatcactta acaagtttta
     2521 ttatcaatta aatttagagc cgtatttctt gatgtttgat ttacttttag cttttattac
     2581 accttttatg ttcttttttg cttctacaat ggtcatattg ggagaaattg atgacagcat
     2641 ttcaagatat ttaatagtaa ctccgcttgg taaaactggt tatcttattt cacgatttgg
     2701 aataccaggt atcttagcat ttattattac tatgattcta cttatctttt tttctctaac
     2761 taaaatttct ctcttgttaa acttatcaat ttctttatta gttttattac aaggagttat
     2821 tatatcaata ttagtgattt ctttatcatc aaataaacta gaaggaatgg taattacgaa
     2881 attctcaggt gtttttatga tgggtatttt agcaccattt tttattttaa ataaagcaca
     2941 atatattctg ttcttcttac caactttttg gatatcgaag gcatttaagg aagataatta
     3001 tgtctatatg tttatttcaa ttgttatatc attaatatgg attttattat tatttaaaaa
     3061 gtttagtaaa aaaatagtta aataagaaaa tttaatatat aaatgtaagg ttgacttgac
     3121 agtaatgttg aaaaatgtta catttaagat gtaaagttaa ccttacatga atggagcaaa
     3181 tatgaaaaat aaaataaaga tattaagaga aaaattagga ttgacacaag aacaactagg
     3241 gagattagtt ggaacatcta gacaggctat aaatgctata gaaactggta aaaatgaacc
     3301 ttcaatatgg ttggcatatg atatttcaag aatttttaat gaaccaattg aaagtgtatt
     3361 tttatttgaa gaaagtgaaa gaaaatcaag agcacagata agtaggggag aatattatgg
     3421 ctataagaaa gataagaaca tttgatgatg aaattttaag aaaaaagagt aagtatgtag
     3481 aaaacgtaga taataagata agagaaatat taaatgacat ggcagagacc atgtacaata
     3541 ctccaaatgg aggaggatta gctgcatgcc aagtaggagt attaaagcga ttagttgtta
     3601 tagatttagg agaaggactt attaagcttg ttaatcctga aattattgaa caagaagggg
     3661 aacaaattgt tgttgaagga tgtttaagtt ttcctgaagt atggggtaaa ttaaaaagac
     3721 caaaaaaagt tacagtgcaa gctctaaacg aatacggtga gaaaatagaa attaaaggtt
     3781 caggttttat ggcaaaatgt ctttgtcatg agatagacca tttaaatggt atagttttta
     3841 cagataaaat tattgaacat gtaaaattgt aagatagatt ggttattgaa tttataaaat
     3901 attttatagt aaggagttaa attatgagat tatggcataa ggatttaatt gatgtattac
     3961 ctaaaaatca attagtaagt caatggagag agctacttgc tataaaaggt tctatagata
     4021 aaaaaggtac tccaaaccat ctattagtga ataaagtatt gaattatagt atagatgaat
     4081 ttaaatttta tacaaaaatt gtacatgatg aaatgttaaa aagaaattat aaacctaatg
     4141 aacttaaata tacaagtata ttaaaatgga aaaatagaaa ttttgcaaat gatatatcta
     4201 atgaacatag tttaaattta gaaaatttat atgatgattg gcataataaa atgtatttaa
     4261 agcaatgctt atataattta gaagaaaaag caacatgtgg aggaatacca ataaatgaat
     4321 ggaatatatt actttgtaaa tatagtaaag attatgaatt gtggtctgga aatatcatgt
     4381 tttaattact atagattaac attatagtat tattgtatat aaagaggtga ttatttgtat
     4441 aataaaataa aatatattcc aataaattgt gaaatagctt gtaataaatt aaaaaggagt
     4501 atgccatata aatgggattt aaatatttat cgtggatgtg agcatggatg taaatattgt
     4561 tatgctattt actctcataa gtacatcaac tccaataatt attttgaaga gatttatgta
     4621 aaaacaaata tagtagaaaa acttgagaga caacttaaaa gcagtaaatg gaagaaagaa
     4681 gtaatcaata taggaggagt tacagatagt tatcaaccaa tagaagctga ctataaaatt
     4741 atgccagaaa tattaaacct gctaataaaa tacaagacac cagctattat ttctacaaag
     4801 tcagatttaa ttttaagaga ttacgattta attgataagc tatctaggat tacatatatc
     4861 aatgtagcat ctacaattac cacagtaaat gaagatacac aaaggcttat tgagccaaat
     4921 ggtgtggatt caatgagacg atttaaaatg ttaaaagaat ttagaaaaac taacgcatca
     4981 gttggtctac atattatgcc aattatacca tatattacag atgattttga taatataaat
     5041 tcgctattta gacatgcaaa agaatgtaat gtacattatg ttttacctgg gactttatac
     5101 ttaaggggta taactagagg tgtttttttt gactttgtta aaaaagagtt tcctgaatta
     5161 tttgataaat tatcaatact atatagtgca ggttcactta atagggagta taaaaatcaa
     5221 ttgtataaaa tggttaatga acttagatat aagtattctc tatcaagcag ttactctaaa
     5281 ataatgaagg aaaagttaaa aaactcagga gatgtacaga tatctttttt tgattagttt
     5341 tatataaaaa aatttttttt actatgatga ttaaaattta ttaaaattaa aatttattga
     5401 caaatataaa atttaaatat ataattaatt cataaaataa aaatatgaaa ggatgatgtt
     5461 gttcggtgat atttgatatc ataaaatcgt tgatataagt attgctggaa aagagtgaat
     5521 aaaatcaaat ttaataagca aacaaagtaa agctatagtt ttattgttgt gcttaaaaat
     5581 atttggttct attttactct tatttaaact catctttttg aagttaaacc acagagtaaa
     5641 ctctgtggtt tttttatttt aaaaataaat tttaaggagt ggtatgtttt gttaaacaaa
     5701 ttatgtatgt atttagaaag accagagctt tataaacaaa gtgaaattaa tttttgggat
     5761 gatgagtata tttcaaagca attattaaaa gcacacttag acactaattt tgaaggagca
     5821 agtagaaatt ttaactttat cgaggattct gtaaattgga tagttacagt tgcaaatcca
     5881 gctaattatc ctaaattact tgatttaggt tgtggtccag gtttatatgc agagaaattt
     5941 gcacaaaaag gttataaggt aactggaatt gatttctcaa aacgttctat taactatgca
     6001 caaaatagaa ataaggagac taatttaaac ataaattact tgttccaaag ttatttaaat
     6061 atgaactata atgaggaatt tgatttggct actttaattt attgtgatta tggagctttg
     6121 tcaactgaaa atagaaggtt attaatggaa aaaatttatg atagtttaaa acctggagga
     6181 aaattaatat tagatgtatt tacaataaat aaatacaata attttgagga aattaaatat
     6241 tgggaaataa atgaagatgg aggattttgg agtaatgaaa aatatatgtg tctacaagat
     6301 aattgtaaat acaatgatta taatacatta gaacaaactc ttgtaataac cgaaaaagat
     6361 gaaaatgtat tttatgtttg gaatcactat ttttcaaaag aaagtttctt attagaagtt
     6421 gaaaatattg gatttaagtc tgttgagttt tttagtaatg ttaagggtga agcatattct
     6481 gacgatagta tgactatggg attagtttta caaaaataat aattttttta aaatgaataa
     6541 ataaaaactt gaaaccttta acaataaaat aacataataa aagcttacat attgaaaaat
     6601 ggaaactttt atgttatttt tagtacatta caaatttgct tatataaaaa tcaatacttg
     6661 aattattatt tttatgtgaa atttatctat agtatatttt tataaacaag taatgttatt
     6721 aaaattaagt atgtagttaa aagtgtatag caaaaaggct gttatattaa tgttagaggt
     6781 tcagggaaaa tatataaagc aaagtattta cagataatgt agattgcaca gctataagtt
     6841 agataataga attatgtaat caagaattta caacaaagta taagataagg ataatgctaa
     6901 attgccatac aggtgctaga tgtgtaaatg gatttaatta atactattga taaagtgcaa
     6961 caagcacata aaccaattga tgagattatt aaaactataa gagatatagt tgacataatt
     7021 aaaatattgg aacttgttta taattttaaa tcaaatcaat aatgattaag attaaaataa
     7081 tattaaggca tcaatgttat aagagtacaa tataaggagt actatatagt actccttact
     7141 tatacatatt aggcaatagg aaattttttc atagttaaat tttcaagtat tttttaggaa
     7201 actttataat tgagaattaa gggtatagta tgtgatgaac ttactgaatt tataattagg
     7261 ttatataatg gaataatcat caatagtttt actttaggga aaatatatgc ttaaggaagt
     7321 atagcttact aatattatta aaatgtatat ttaaaatgat aaaaaaagaa ttagacaagt
     7381 atttagtaaa ttgactatgc aaaattctat caagcaggaa ccctatgact ttagtcatgg
     7441 gaggttcagt aggtaacttg ttgaattaag tttatattat ttaaataaac tacttaattt
     7501 agataatata aaaagtcaga attttaagaa tagagctata taatcttagc tattaggaga
     7561 attactatga taggaatata ttttagtgga acaggaaatt caagatactg tgtagaaaaa
     7621 tttttacaag aatatgatat aacagcaaat tcttattcta ttgaagataa tgaactttta
     7681 cagcacatca ataatcatga gaatattatt tttagttatc cggtgcaata tagtaatgtg
     7741 ccaaaaatat tgaaagattt tataataaat cattcaactt tgtggaatgg taaacgtgtt
     7801 tttattattg caactatggc tctatttagt ggagatggct ctggtgtgtt atctagaata
     7861 ttaaaaaaat acggtgctat aactgttggt ggattacatg tgaagatgcc agatagtata
     7921 ggagatgaaa aggtattaaa acattcttta gagtataata gaaagcttgt aataaaagca
     7981 gaaaagaaag taactaaagc tgctaaaaaa ctgaaacatg ggtcaccacc aaaagaaggg
     8041 ttaggttttt tgagtcatat tgtagggtta tttggacaac gattgtattt tatcaacaaa
     8101 acaaatatgt atacagataa actaaaaatt aatactgagg aatgtattgt atgtggtaaa
     8161 tgtgttaatt tatgtccaat gaagaatcta gtaattaaaa attgtatgat tgttgcaaat
     8221 gggcaatgta caatgtgtta tcgttgtgta aataattgtg ctaaaaaagc aattacattg
     8281 ttaggtaaaa aagttgttga acagagtaat attaaaaaat atatgtaaga aatttaaagt
     8341 tttattgaat gtaaattgat gtatatttta tgttagaaag agaggttaaa taatggaata
     8401 ctacatagtt gattcatttg caactaaact atttaaagga aatcctgctg gagtttgtgt
     8461 tcttgacaga agaatacctt tagagttaat gcagaaaata gcagaagaaa acaatttacc
     8521 agaaacagca tttgtagtta aaggtaaagg aaattatgaa ttacgatggt ttactcctaa
     8581 agcagaaatt gatttatgtg gtcatgcaac tttagcagca gcttatgtaa tatcaaattt
     8641 tattgatgtt aatgtaaaaa ggatagattt ctttactcaa agtggaaagc ttgaagttac
     8701 acgaaatgga aatctatatg aaatgatatt tccagaaata atgcctattg aaatagagtt
     8761 atcaccacaa caagctaatt tgataggctg tgtaccaagt gctgtttatt cttcaagaga
     8821 tttaatctta ttgctaaaca gtgaacaaga agttataaat tataaaccta attacgcaca
     8881 attgcgtaaa ttgacagact ggttaggtat aataatcaca gcacaaggaa gtaatacaga
     8941 ttttgtatca agatattttt gtccagaatt agattcagaa gaccctgtta ctggttcatc
     9001 acattgtaat cttattccat actggtctga aaagttggga aaacacaaaa tggtagctgc
     9061 tcaactttct aatcgtggtg gtataattca gtgtgaagtg ttgaaggata atactgtgaa
     9121 aatttctggt gaagcagtct tgtttatgca gggaacaata aagattgata tttaaataag
     9181 tataataact aatttattat gaaaaataat taattttctt agtgttaaca ataataaagt
     9241 agagttaaaa aacttgattt cacttaaatc aatatgatag atgttgatac taagattgac
     9301 aagcatgatt aaaaaagata atatttaatc tataaagata aaaacacagt ctggttggta
     9361 gtccagacgc agcaatagct gtcagtaacc tgcctccttg gttgtccgtt ctttagaaag
     9421 taatttaagg aggaactagc aatggacaaa attagtggaa aattaacagt cttatttgaa
     9481 gaaccatttt gggtaggtat atttgaaaga caagatggta aaaaatatga agcatgtagg
     9541 gtagtttttg gagcagaacc taaagaagta gaagtgtatg aatttatact tgaaagattt
     9601 ttctcattag attttggtag cattaaatta gaaaaaaatg tgaccaaaga caacattgga
     9661 tacaaaagaa tgcagagaaa agtaaaaaag gaacaagaaa aagaaactat agggactaag
     9721 gctcaaaatg ctcttaagct tcaatatgaa gaaagaaaac aggatagaaa aaacctagct
     9781 aaaaatagaa aagaagaaga aaaagaaaga ctatttaatt tgaaacaaga aaagagaaaa
     9841 gctaaacata agggtcatta attttaatta aatatctttt taataattgc tagaaaaagg
     9901 tgtataaaat ttttaatttt atacacctta atttattgaa aagaataagg ttttatattt
     9961 caattacttt aaaatataaa aatatactaa taaaaattgt aaaaatagta taaatggtat
    10021 accaaatgta aatttaatgt gtttagtttt atgtctaaaa gaatacattc caattataga
    10081 acctatacta cctccaatta ctgctataga cataagagta gcttctttta ttctccattc
    10141 gtttctaatt gctcttttct tatctatgta cattgaaaag aaagcaatag aattaatcac
    10201 tataaaatat attagaataa aatttttcac agtcttgcct ccataaaaat atattataca
    10261 ttataaaaca agatatttgt tttggaaagt taaatttgta attagtacat ataaaatatt
    10321 tgataagaac aaatataata ttaattggaa aatttaaaaa aattaaacaa ttagcaaata
    10381 gggggtatat ttataagaca caaagtgata aaatctattt agaaaataat gtaaaataac
    10441 aggaggataa agcaaaatga aatttatagt agaaaaagaa gtttttgata aattagaaaa
    10501 tgtatgcttt ggtgtagtag ttgcaaaagg aatagataat actaaagaaa tagaaagaat
    10561 aagcaacctt ttagatataa gtatagatag agtagaagat tacttcaaag ataaaaaagt
    10621 aaaggaatca gaagaaatta taccatatag agaggcattt agaagtctag gcatgaaccc
    10681 aaataaattt atgagttcga tagaagcaat gacaacaagg gttgctaaga ataagaaact
    10741 tcctcatata aatcctattg tagatttagg aaactctatt tctttaaaat atttacttcc
    10801 aatgggtgct catgatatgg attttagaaa tgatgatgta tatgttagat tttctaaaaa
    10861 aggggataaa tttgtaccat ttggagaaac tgatgttgaa ttaatggaag aaggagaatt
    10921 aatttattca gttggagaca tggtaaaaac tagaagatgg atatggagac aaggagaaga
    10981 aggaaaaatt actaatagct caaaaaatat attttttcct atagatggtt ttactgatgc
    11041 taatttagat aaggtaatga gtgcaagaga agaattagca aaattgttaa aggaaatatt
    11101 taattgtgaa attaaagtag gttttgtaga taaagataat cctgaaatgg aaatataata
    11161 agttgttgaa tacttgttaa gatattatat ttacttaatg atgttaaaaa ggtattaaaa
    11221 aagaaaggtc gtagtaaatt tgaaaaaaac tataataaag aatgtaaatc caatgattga
    11281 aaataagttt ataggacttt ttgaaataga gtataaaaac aaattagatg aagataaggt
    11341 ttggatggta gcatcaagaa aaagtagtga gcaattaaaa aatatttatt tgaagaatga
    11401 agaagatagt gtagatgcag ttgtaatagt tgggcttcat aaaagtagta aaaaattaat
    11461 tttaataaga caatttagag tacctattaa tggatatata tacgagttac cagcaggtct
    11521 tgttgatgaa ggagagtcta tagatatttc cgttgaaaga gaacttagag aagaaacagg
    11581 acttactctt ttagaaatta aaaaaaataa aagtattgat aaagtttatt tatctcctgg
    11641 aatgagtgat gaatctgttg cctttgttta ttgtatatgt gatggtgata taacagatga
    11701 atttttagaa ccagatgaag agattgaagc gcttttaatt tcgcaagaag atgcaaaaga
    11761 gattttacaa agtaatcata agatagacac aaaagcattt ttgattttac aaatgtttgt
    11821 gagtttagga gctaaattat ttgaatagat atatagaaaa gaggttatat agaagagtaa
    11881 aataataaag tttattttac ttgtgtaaat tataatctct ttttttatac aataaagtat
    11941 aattttgtgt atttttaaat aagtgatttg actcttaaat tttcataata tgttataaac
    12001 caatttaaga tactacaata attaaaattc caaaattatt taacatattt gcaattaaaa
    12061 tgagaaaatg taaaggctga atgattgaaa aatttgatgt aaaatagtag cttagtttaa
    12121 tactatagtt tgtagggtta aaaattagaa attgtaaggg agataaattc tatgaaaaaa
    12181 ttagttatta ttttaattag tatcattagt atggttagct tgacagcttg taaaaaaagc
    12241 aatgaaagta aatataataa tttaaacaac aaagaatccg gacaaacaac cattgaaatg
    12301 gacttagata aaaattatga tacttcagac ccatttgtta atgcacgttt gttttgtgtg
    12361 tctaatgata tagatgtttt agacacagag atttctttta aaatggatgg agatagtggt
    12421 attgtggaaa taaaggacaa taaaacagat gaaactttat ggagtaatac atggcatgga
    12481 agcgttgata atgatacatt cacaatttct ctttctaata tacaaaaaga gaaagagtat
    12541 gctatatgct ttactggaat taaaataaat catgcagttg taaaggttac ttttgaaagc
    12601 aatttagtta aggagaaaga aaaaccctca aaataaattc cagcttttct ataacttgat
    12661 attcagtaca taaaagcagt tgatatttat gattgactgc tttttcttat caaaaaggat
    12721 aggttgtgca tttttacttg aaatagttta tgcacatcaa aagttggtga gcatgatatt
    12781 ggaaatggta agcatgaaat cttgataata caaggaaata aatttaagtt ttcaattaag
    12841 tatatttcca taatttagtt aacaataagc taattattat ttatattttg atatataaat
    12901 aataattgta gcaaatttta aaaaatcata tattttttga actaaataaa aatttatttt
    12961 tattatttta aattagagga gattcttgaa gatatattat ttttaaaaat aagtatagtt
    13021 taaaaattca aaaaaaagat ggcaaaatat gtcccaaggg acattgagta aaataaatga
    13081 tttcagctta tttacactaa aaagtacata ctaaatgagg ggtaaaataa agaaaaatga
    13141 tatactaatt gtcttttgat aataaaaaaa catggaattt gattatttaa tcaagaaaaa
    13201 gtattgaaca aatgatttat ttagtgtaca ataatcatgt aaaaacatag taatggaggg
    13261 agacatttca taatgaaaat taaggtagga atgaacggat ttggaagaat aggaagagct
    13321 gtattaagaa tagctcaaga agaattagga gataatatag aaattgtagc cataaatgca
    13381 agagcaacaa cagaatcttt ggcacattta tttacatatg attcatgcta tggaactttt
    13441 agaggtgaag tagaggcaaa ggatgaagac actctagtag taaactctaa agagataaaa
    13501 atattaagat ataatgaccc agaagaatta ccttggaaag aattgggggt tgatatagta
    13561 atagaatcaa caggattatt tacacaaaga gaaaaggcag aaaaacatat taaagcagga
    13621 gcaaaaaaag taattataac agctccagga aaaaatgaag atataactat agttatcgga
    13681 gttaatgaga aagaatatga taatgaaaag cacaatataa tttcaaatgc atcttgtact
    13741 acaaattgtc tagctccttt tgctaaagta ttggacgaaa aatttggtat agtaaaaggg
    13801 ttaatgacta cagtacattc atatactaac gatcaaagaa tattagataa aagccataaa
    13861 gatttgagaa gagcaagagc agcggctgaa tctataatac ctaccactac aggagcagca
    13921 aaagctgtgt caagagtact accacaatta gaaggaaaat taaatggttt ttcacttaga
    13981 gtaccaacac caacagtttc acttgttgat ttagtgtgcg aacttaaaga aaacgttaca
    14041 tctgagcaag ttaactctgt gttaaaagat gctgctgaag gagaattaaa aggtgtttta
    14101 ggatattgtg acaagccact tgtttctata gattataaag gagattcaag atcatctatt
    14161 atagacgctc tatccacaat ggttatagaa gacaatatgg ttaaggtagt ttcttggtat
    14221 gataatgaat ggggatattc ttcaagaact gttgacttag taaaatatgt agctgaaaaa
    14281 ttaaaataat atatatttct taaagcaaat tttaaaaaaa ttttttaaaa aaattatata
    14341 aaactaaaaa aaaagattat gaaaaatatt gaaggaaaaa ttttttcata atcttttttc
    14401 tttcatgcaa aaagatacaa gttacaactt atatttgcat attaggtcgt tagagaagta
    14461 tatatatatt tagtaaggaa tcatactttt tataaaatga aagataagat gtcgaaaact
    14521 tgcataaatc tgaaaaattt ttattattaa aatactgatt tccattttaa aaatgataaa
    14581 aaaatgacaa ataaagttgc aaatagtaat acttataagt ataattaaaa aagagaaaag
    14641 gttttgttaa actttataac aaaaaaagaa gggatgattc aaaatggaga agttaacaga
    14701 caggagacag gttaaggcag taagcatgga aaccttcatt ttcctaatat tactagtagt
    14761 tggttttggg tatgttggta gtattatggg agcaggaatg atgttcaaag taataatgag
    14821 tacagcacat gctttactat tggagacagt tttcttaatt atggctatgg cagttcttgc
    14881 aggcgcatta agcgcactac tttcagagtt tggagtaata gcgttaatta ataaaatttt
    14941 cgctgtattt atgaagcctt tatatggact acctggtgct agtatagcag gagctataac
    15001 aacttattta tcagataatc cagctattat accttttgct aaagataaaa cattcacaca
    15061 gtattttaaa caataccaag ttccagcatt atgtaactta ggaacagcct ttggtatggg
    15121 acttatctta acaacattta tgatttctca aggtacagaa tatgtacttc cagcattaat
    15181 tggtaattta ggtgcaatta taggtagtgt cataagtgtt agaataatgt tgacatttac
    15241 taagaagttt tataaataca atccggaaga agataaagca acaggaacat tagagaaaaa
    15301 ggatgaattt agagaaatca gagaaggaaa tgtatttcaa agagctttag atgcaattct
    15361 tgaaggtggt aaaatggggg tagatatggg aatggctata attcctggag tcctagtagt
    15421 atgtacattg gtgatgttat taacttttgg accatctaca gatcctgtta caggtcaaga
    15481 agtttataca ggagctgcat atgaaggaat aaaattactt ccagttattg gagataaact
    15541 tggatttata ttagaaccat tatttggatt tacatcacca gaggcaatag cattccctat
    15601 aacttcattg ggagctgttg gcgcagcaat gtctctagtt ccagagttta taaagtcagg
    15661 agctataact cctaatgata tagcagtatt tacagctatg ggtatgtgtt ggagtggata
    15721 tttaagtaca catattggta tgatggatgc attaaatgct agacagttgg caggtaaagc
    15781 tattctttct catacaatag gtggtttatg tgcaggagca gctgctcact ttatatttac
    15841 actcgtagga taaatgtaat cagctaagat aaaaaataat atacaaaaaa tggttgttga
    15901 tatgtaataa gaatttacat atcaacaacc atttttttct gactaaagat aatttataat
    15961 acataaacat taagtggata ttagagttaa gttatattta tgtaaaaagt aacaaaaatc
    16021 tgtagaaatt tatgacaaaa tttagtacac taactatatg ttcttattta aaatttaagg
    16081 ttagaattag gaattaccta gtagagtaag ctttgattaa taaaagaatc ataaaataaa
    16141 aatattagtc ataaagatta taaataactt acttaaaata aagccataga acttacaaaa
    16201 attaaaaaag catataaata tggaggggta caatggatga attatttcat ataggaattg
    16261 atgtaggttc tacaacggtg aaggttgtgg tacttaataa tagtaataat atagttcata
    16321 aagaatatag aagacattat tctgatgtaa aaaaatcagt taaagaagta ctaaatggta
    16381 tatatgaaaa attgggagat ataaatacta ccattataat aactggttca ggtggaattg
    16441 gaatatcaaa aaaattaggt gtaaaatttg ttcaggaagt tatatctagt acaaaatcaa
    16501 tagagtattt tcatcctgaa actgatgttg tcatagaact tgggggtgaa gatgcaaaga
    16561 taacatatct aagtggagga atagaccaaa gaatgaatgg aatatgtgca ggtggcactg
    16621 gagcattcat agaccagatg gcatcacttt taaagacaga tgccagtgga ttaaatgaac
    16681 ttgctaaagg gtataatgtt atatatccca tagcttcaag atgtggtgtt tttgctaaaa
    16741 cagatattca acctcttata aatgatggag ccaatcagac tgatatagca atgagtattt
    16801 tcaatgcagt tgtcgtacaa actgtgagtg tcctttcttg tggaagaaaa atagaaggaa
    16861 atgttgcgtt tttgggtggt ccattgtact ttttatctga acttagagaa gcatttaaaa
    16921 gagtattaga tttaaaagat gaaaacataa tcttcccaga aaatgcacaa ctttatattg
    16981 ctattggagc aggattatta tcagtagatg aagataatat acaactaaaa tctctaatag
    17041 aaaaattgga tagtataaag aatattgaag atggagaagt aaatttatta gaacctttat
    17101 ttaaagataa aaatgagtat gaagaatttt caagaagaca tgaaaaagaa aaaataaatt
    17161 atgtggacat aaattcaata aaaagcaatt gttatttagg aattgatgca ggctcaacta
    17221 caactaaagc agcacttatt gatgaggatg gaagacttgt atattcttat tataatagca
    17281 atgaaggaaa tccattaaaa acaaccatta aagttataaa tgaagtatat gatatattac
    17341 caaaaaatat taaaatattg agttcaactg taactggata tggagaaggt cttattaaaa
    17401 aagctcttaa aatcgataat ggagagatag agactatagc acattacaaa gctgctaaat
    17461 ttttcaacaa agatgtagat tttatacttg atattggtgg tcaagatatg aagtgcctaa
    17521 aaataaaaga tggagtaatt gataatataa tattaaatga ggcttgttca tcaggttgtg
    17581 gctcattttt agagacattt gcaagttctt tatctatgac aatagaagaa tttgcgcatg
    17641 aaggtattta ttctcaaaat cctgtagatt taggttcaag atgtactgta tttatgaatt
    17701 ctagagtaaa acaagctcaa aaagaaggtg caagtgttgg agatatatca gcagggctat
    17761 cttattcagt aataaaaaat gctttattta aagtaataaa gattagagat ttaaatgaga
    17821 ttggtaataa tattgttgta caaggtggaa ccttttataa tgatttggtg cttagaagct
    17881 ttgaaaaact aattggaaaa aatgtaatta gacctaatat ttcaggaatt atgggagctt
    17941 ttggttcagc acttatagct aaagaaaaat atgaaagtgg atacaaaaca tccttgttat
    18001 ctaaggaaga gttgaataat atcaagctag aagcaagtgt aactagatgt aaaggttgtt
    18061 caaatcattg tcttcttaca ataaataaat tttctgataa tgaaattttt atttcaggta
    18121 atagatgtga gaaaggtgaa gctatttatg gtgataaaaa aataggatta gaaaagaaaa
    18181 aaccaataaa tttatttaag tacaaataca atagaatttt tagatacaaa ccattggaaa
    18241 aagaagaagc aaaaaatggg gaaataggga taccaagagt attaaatatg tatgaagatt
    18301 atccattttg gtttactttt ttcaatgcta tgggctttag agttgtactt tcagataggt
    18361 catcaaaaca gttatatgaa actggtataa ctagtattgc atctgaaaca gtttgttatc
    18421 caggaaaatt agttcatgga catatagaaa atttaattgc aaaaggtata aaaaatatat
    18481 tttatccaag tgtaacaaat gaaaataaag aggattcaaa tgcagataat tattataatt
    18541 gtcctgttgt aatatcatat tctgaagtaa taaaaaacaa tgtagagaat ataagaaata
    18601 agaacataaa ttatataaat ccatttataa gtctaaatga caaggaaaaa ttgaagaaaa
    18661 gactctatga tgaattatca agacattttt ctgatataaa aataactaaa gaagaagtta
    18721 atcatgcagt tgataaagct actgatgaac agaatttatt taaaatggag atacaagcag
    18781 caggtgaaag agcacttaaa gaaataaaag aaagaaatat gaaggggatt gtgttatgtg
    18841 gaagaccata tcatatagac ccagaaataa atcatggaat gccagagctt ataaattcat
    18901 tagatatggc agtgcttact gaagactcta tatgtcattt agccaatgta caaagaccac
    18961 ttagagttgt agaccaatgg gtatatcatt caagattgta taaagcagct agttttgtaa
    19021 gagataaaaa ctatttggaa cttgttcaac ttaattcatt tggctgtgga ttagatgcag
    19081 taactacaga ccaggttcaa gaaatactta atgaaaaatc gaagatatat actattataa
    19141 agattgacga aggcaacaat ttaggagcag ctaagataag gatgaggtcc ttaaaagcag
    19201 ctatgtttga gagagaaagc aaaaatatag atgtaaaaaa tataaaggta aacaggatac
    19261 agtatgctaa aaataatcag ataacgaaca aacatactat attagcacca caaatgtctc
    19321 caatacattt ccaattcctt gaaaaagctg ttaacttaag tggttataat atagaaattc
    19381 taaaagatac tgatagtagt gtaattgaag aagggttaag atatgtaaat aatgatgcat
    19441 gttatccagc tataatagtt gtaggtcagc taataacagc attaaaatct ggcaaatatg
    19501 atttaaataa cacttctgtt actataactc agactggtgg aggttgtaga gctacaaatt
    19561 atattggatt cttaagaaag gctatatacg atgctggatt taaagatatt cctgtcattg
    19621 cacttagtgt aaatggaata gaagatagtg gtataatgga taatatatca ctaaaattaa
    19681 tcaatagact ctttatgtct gttgtttatg gtgatttgct gatgaaagta ctatatagag
    19741 taagaccata tgaaaaagtt actggaagtg caaatgctct atatgaaaaa tgggttgata
    19801 tatgcaaatc ttctcttgtt aaagcaaaaa tttctgtatt tactagaaat ataaaagata
    19861 tagtaaaaga atttgataat ttagagatac tagatatcaa aaaaccaaaa gtaggtttgg
    19921 ttggagaaat attggtgaaa ttccatccaa ttgcaaataa taatttagta gatatattag
    19981 agcgagaagg tgctgaagca gtcgtgccag accttacaaa tttcttctta agttgtgcct
    20041 ttaatactat atacaaacac actcatttag aaggaagtag aaagagtaga atgataggag
    20101 aagcatttat atacataact ggaatatatc aaagagtata taaaaaagca cttgataaga
    20161 gtgagagatt ttatgctcca gccaatataa aaaatgttgc taaaagtaca gaaccagtag
    20221 tgtcattagg taatcaaact ggagaaggtt ggcttttaac aggtgagatg gttgaattat
    20281 taaatgaagg tgtagaaaac attatttgta tgcaaccttt tggatgttta cctaatcaca
    20341 taattggtaa gggttctata aaagaattaa agaggttgta taaaaatgca aatataatac
    20401 ctattgatta tgacccttct gcaagtgagg taaatcaact taacaggata aaacttatgc
    20461 tttctaaggc ttttaaaaat atataagatt tatattttga tatgttaaaa gttaatgaat
    20521 ttaaaaatat actctatatt aagtatctat aaaaaagagt tatcctattt tttagggtaa
    20581 ctctttatta gttaaaaaat tatatgtata ctgatattaa aggggggaag taatatgagt
    20641 ttagtatatg atgtttataa atcaaaatta ggaaatttgt acatattaag tgaagatggt
    20701 tttatagtta gtatatacat aggagatgaa aaatttaata atttaaaaaa tgaatgtatt
    20761 aaaaaaggaa ttgatataaa gagaagtaag gaagatttaa aggaagcaat aagccagata
    20821 gatgattact ttaatggaaa aagaaaagtc tttaatttaa atattcgtat tgaagggact
    20881 gaatttcaaa aatcagttta tagggaaatg ttaaaaatac cttatggaga aacattgtgt
    20941 tattcagata tagcaagaaa tataaacaat ccaaaatcag taagagcaat aggacaagca
    21001 agtaaaagta ataaaattcc aataataata ccttgtcata gagtagttgg caaaaaagat
    21061 attggtggat atatggggaa tcattctgat ttgaaagaga tactattaaa tttagaaaga
    21121 cagagttaac ttagaaagaa atgtttttat tgttaatatt tgatataata atagtaaaaa
    21181 gaagtatttt ttacaaaaac aatgaagaat ttattttaat ttataaaaat gagggggaat
    21241 tttaatgtgt gataaagtta tagcatcaaa atatcatgag aagggattta attgtgcaga
    21301 atcagttata aaagcatata atgaggaatt taatacagat ataccagttt gtttaggaag
    21361 tggactgggt tcagggtgtg gggttgctag tttatgtggt gcagtaaatg catcaaatat
    21421 aataattgga tatgtaaaag gcagaaacca cgaagatgaa agtactaaag ctaaagtata
    21481 tgctaaagat ttaactacaa cagtaagaaa agagtatggt tcagagttat gtatagacct
    21541 aaaaaaagat ttagttgctt gtagagaaat aatggatttc gcatatgata gtctaaagga
    21601 aactttaaaa aaagaattat aagaaaataa gctatcaagt ataaattatt tgatagctta
    21661 tttttatagt cgatactaaa taaatcaaat atattttaga attattattt gtaaataaac
    21721 agcaaataat tttaggattt ttatttaata tagtttttat ttatatttat ttttgaatat
    21781 gttatacttt gtttaaataa atatttgtaa tgagggagat gagaagattg aagaatctca
    21841 atttacttat taataaatta tattcaaaaa atcataatga agcatataaa acttttttat
    21901 tcttagaaaa tgaaagttta aaatcaaata ttacatattg tttttttgac tcattcctag
    21961 aaatgattga taacgaaaac tcatatattc gaacaagggg attgttatta atctctgcaa
    22021 atgctcagtg ggatattgat aataaaattg aaataaatat agatagtata ctaagtcata
    22081 tcgtagataa aaaaccttct gtatcaagaa tgtttattaa atccatacct aatattacaa
    22141 agtataaaga aaatcttatt aatcgcataa aaatggaatt aagtaatgca gatataagta
    22201 tttataataa taatatgaaa ccattagtag aaaaggat
//
LOCUS       DAMKMI010000057        21946 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMKMI010000057 DAMKMI010000000
VERSION     DAMKMI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21946
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.058"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(183..890)
                     /locus_tag="PC429_001971"
     CDS             complement(183..890)
                     /locus_tag="PC429_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3847476.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYKWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYAKRLITATRVLQ"
     gene            complement(1089..3002)
                     /locus_tag="PC429_001972"
     CDS             complement(1089..3002)
                     /locus_tag="PC429_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF3847477.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKYN
                     VDEIIFSIANIEKRRKKDIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            complement(3086..4177)
                     /locus_tag="PC429_001973"
     CDS             complement(3086..4177)
                     /locus_tag="PC429_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3847478.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            complement(4183..5550)
                     /locus_tag="PC429_001974"
     CDS             complement(4183..5550)
                     /locus_tag="PC429_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF3847479.1"
                     /translation="MYFVSMFLMPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLA
                     EVGNENVVCVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDV
                     DVILIAVGTPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYIN
                     RNLKNDVKVELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPII
                     VTNRNSAEMIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLK
                     AGIGYGGSCFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQD
                     MKVAVLGLTFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYA
                     DSVDDAIEDAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEY
                     YSIGR"
     gene            complement(5504..6256)
                     /locus_tag="PC429_001975"
     CDS             complement(5504..6256)
                     /locus_tag="PC429_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3847480.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            complement(6304..7212)
                     /locus_tag="PC429_001976"
     CDS             complement(6304..7212)
                     /locus_tag="PC429_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3847481.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            complement(7234..7983)
                     /locus_tag="PC429_001977"
     CDS             complement(7234..7983)
                     /locus_tag="PC429_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3847482.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            complement(8012..9187)
                     /locus_tag="PC429_001978"
     CDS             complement(8012..9187)
                     /locus_tag="PC429_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF3847483.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            complement(9202..10041)
                     /locus_tag="PC429_001979"
     CDS             complement(9202..10041)
                     /locus_tag="PC429_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3847484.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            complement(10044..11201)
                     /locus_tag="PC429_001980"
     CDS             complement(10044..11201)
                     /locus_tag="PC429_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF3847485.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            complement(11217..11939)
                     /locus_tag="PC429_001981"
     CDS             complement(11217..11939)
                     /locus_tag="PC429_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF3847486.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            complement(11951..13024)
                     /locus_tag="PC429_001982"
     CDS             complement(11951..13024)
                     /locus_tag="PC429_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF3847487.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            complement(13046..14752)
                     /locus_tag="PC429_001983"
     CDS             complement(13046..14752)
                     /locus_tag="PC429_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF3847488.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            complement(14798..16354)
                     /gene="murJ"
                     /locus_tag="PC429_001984"
     CDS             complement(14798..16354)
                     /gene="murJ"
                     /locus_tag="PC429_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF3847489.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            complement(16469..17524)
                     /locus_tag="PC429_001985"
     CDS             complement(16469..17524)
                     /locus_tag="PC429_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF3847490.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            complement(17619..18305)
                     /locus_tag="PC429_001986"
     CDS             complement(17619..18305)
                     /locus_tag="PC429_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF3847491.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            complement(18525..20549)
                     /locus_tag="PC429_001987"
     CDS             complement(18525..20549)
                     /locus_tag="PC429_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3847492.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            complement(20803..21915)
                     /locus_tag="PC429_001988"
     CDS             complement(20803..21915)
                     /locus_tag="PC429_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF3847493.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLCIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
BASE COUNT         6851 a         4052 c         2290 g         8753 t
ORIGIN      
        1 ttgattataa tatgaaataa tttattataa aaaaaacagc aaagaaattt atcttctttg
       61 ctgtttttat aacgttcatc actattttaa tattactaaa ctcttaaatc tctatgtaaa
      121 acattaaatg taattatata tttaatgtta atattcaatc ctatgtaaat caatatctat
      181 tattattgta aaacacgtgt agcagttata agtctttttg cataattagt tcctggagcc
      241 atgctagtaa cttttactac atctcctgtt tgtggtgaat gtatgaattt agaatctcct
      301 acatacaatc caacgtgatt tattgaacca cctgaaccaa agaaaactaa atctcctggt
      361 tttaattctg ctcttgatac tttctttcct actgttgctt gtgatcttga tactcttggt
      421 aaattaactc ctgctccatt tttatagaca taagaagtga acccagaaca atcaaaagaa
      481 tttggtccag ttgctcccca tttatatggc ttaccttgtt gcttaaatgc taagtttaaa
      541 acattttgta cctttgcatc tacatttcca ccttgaatag ttaaataatc tccatttacc
      601 catcctgtaa cattgtcaac actttttatt ttaatccatc catttgaatg taattctaaa
      661 acttcaactt tgtctcctgt gtttatcttt cctactactg catcatttgc actagctcca
      721 cttcttacat ttaaaaaatc acactggtct actgttgcac tcataggtga attagttggc
      781 tctgctgcac tagccgttat tgctcctgct tctactgtga caactcctgc tattaacacc
      841 atagctataa attttttcaa tatatgtttt ttatttttaa tattcatcat aatctcaaat
      901 acctaatcct ttcgaaaatt taacattttg ttccttcgtt tccaattcct aattaacatt
      961 ctatcttaca tcaaaataaa ttgcaacctt ttttgtaatc tttttgtaat ctttttgtaa
     1021 tcttttgttt ctttttagta tatatttgtt tctttttgct ccccctggtt acaaattaga
     1081 aatatattct acttttctct agttgttgca atttctttga tattagcatc ttctggttta
     1141 atataagttg gtactaattc cctcataata ccatctattt tttcaacctc ttcattgtta
     1201 gtaatttctc ttaaaagttt taagtatttt gttatttttt ctctatcaac atctattggt
     1261 ctaccaataa aaatcttttt atgctctgta tccaatagac cttcttctga cataagtagt
     1321 tcttcataca acttttctcc tggtctaaga ccagaaaatt caatttttat atcaacatct
     1381 ggctcaaatc cactgaattt aattaagttt tttgctaaat ccaatattct tacaggttct
     1441 cccatatcaa gcacaaatat ttctccacct ttagccatag ccccagcttg tataactaag
     1501 cctactgctt ctggtattgt cataaaatat ctaataatat ctggatgagt aactgtcact
     1561 ggtccgccat cttcaatttg cttcttaaaa agtggaatta cactaccatt actaccaagc
     1621 acatttccaa atctaacagc aacaaattca gtctggcttt cagcattcat agtctgtata
     1681 atcatttcag cagctctttt agtagctccc attatgttag tagggtttac agccttatct
     1741 gtagatataa ggacaaattt cttagctcta tattttgaag acaagccagc tatgtttaaa
     1801 gttccaaata tattattctt tatagcttcg ccagggctcg attccataag aggaacatgt
     1861 ttatgtgctg ctgcatgaaa taccacttct ggtttatatt tattaaatat ttcatccatt
     1921 cttttttctt ctcttattga tgctattact gtcttcaaat ctaatttatt tttatatttt
     1981 ctaagtaatt cttgttgtat tgagtaagca ttattttcat aattatcaac tataataagc
     2041 tctttaggat taaatcctgc aatctgtcta caaagctctg aaccaattga gcctcctcct
     2101 ccagttatta ggattacttt tccctcaatg taattactta tctctcttaa atttgttttt
     2161 ataggctctc tacccaacaa gtcttctatc tctacttctc taatctgttt tatatcaact
     2221 tttccatcta taatttcgta aattcctggt attgtcttta ttttacagtt agtgttttta
     2281 catatatcaa taatatcttt ttttctacgt ttctctatgt ttgctattga aaagattatc
     2341 tcatctacat tgtacttttc taccacatct tttatatctg aagttgttcc tacaattttc
     2401 acattatgga ttattcttcc aattttattt ttatcatcat caattatagc cactgctact
     2461 tttttaagtt ctggatttct ttttagttcc tgtataacca tttctcctgc atctcctgca
     2521 ccaattataa ggatatttga aacctttcct ctagaatatg tcttgttcat gactattctt
     2581 atagctctat aggctattct tgcgccactt gtaaacatta taataaatat tgtatttaat
     2641 acataaaaca ttataggata atcaaaccct aataatttat ttattatata aatcggaatt
     2701 aaataagcta aagttgccac aaaaatagat ataatttctt cttctcctgc atatctccat
     2761 aatgtgttgt agcacttaaa caatctaaat gatactatat gaagtactat atatatacac
     2821 actgaaatta ttatatcatt agtggtaaat gcatttaaat tacctgtcaa gcttaaagac
     2881 attcgaaacg ctactagtac gctacaaata tctagaatta atagtgttaa atgtctaaat
     2941 ttatattttt taaaaagcct attaaaaaag ctttctttct ctaaatctat actttctcgc
     3001 ataactagaa gccctccttt aaacagcaaa agctacgcac ctaagcatag ccaacaacat
     3061 ctatcttttg ctgtattttt tcattttatt ttttatattt atcaaatata gaatcataaa
     3121 ttttttctat atcattaaag tttaaattaa cctcataatt ttctctaaca aattgacgcc
     3181 ctttcttacc catattaagt ctaagttcat cattttccat tagttttatt atagcatttt
     3241 ttatttcttc tggttgtttt ggttctacaa ttaagccagt ttcattttcc catacagatt
     3301 ctggatgtcc tcctacattt gtaactataa caggtacttc acatgcttct gattctatag
     3361 ctgccactcc aaatccctct cttaaagaag gaaatacagt tacatcaaaa gaattaaaag
     3421 tcttgcttac attttctgga gatattcttc ctaaaaactg aacatcattt tctataccca
     3481 attctttagc taaatttatt aggttgtcca tctgacttcc agagccacct attttaagaa
     3541 ttattttctt atctttatat tcatctttaa gcatcttaaa tgcttgtatt aaatattcta
     3601 ttccatattt tttttctaaa ctctttataa ttcctattac aaaagcgtct tctttttcta
     3661 cttccatagg cttaaaccta tccatatcta ctccaaaaaa ggttacatct acatgtttag
     3721 ctgtatattt attagcctct ttagccatat ctctactatt tgaaaagata tagtctgctt
     3781 ttctaaagtt atgttttata atttgttttt gtataaaacc attattaggg aaatcataaa
     3841 tatcagtacc ccaaacagaa ataatatatg gatgataccc aagtagactt cctataaatc
     3901 cataactact tgcatactgt gcatgcaata tatctggctt tatttcattt actagcttct
     3961 ttatctgctt tattgaacct aagtatcccg tttttttaga aatattttca ttttttagtt
     4021 cttctacatt agaaccaaaa ttataaattt ttattccttc catgtgacct ccatttaaag
     4081 agattacatg aatgtcatat cctttatttt taaaaaaact acaccatttt aaagtatgag
     4141 gatttgaccc atcagctaaa taacaaacag tcttcataat atttaccttc ctatcgaata
     4201 atactcaatt cctaactctt ccatatcttt aattttataa caatttcttc catcaaggac
     4261 taaaggattt ttcatctttt ccttatactt atgtatatca taatttaata catctttcca
     4321 ttctgtcatt ataaaacata tctctgcatc ttctatcgca tcatctacag aatcagcata
     4381 aagtatagag tcaccaaata ttttcttagt attatttaca ccaattgggt cgtatacagt
     4441 aactcttgcc cctctttcga gcaaatattt tatatttggt atagatggtg cttctcttat
     4501 atcatcagtg tctggcttaa aagtcaaacc taaaacagca accttcatat cttgaaagct
     4561 ctctactaac ttagatgcct ttctaactaa tttaagtttt tgactttcat taacttctat
     4621 tgcagctttt actgttttta attcataacc attatgttca gataaccagt gaagagcttt
     4681 tgtgtctttt ggaaagcaag aaccaccata tcctattccc gcctttaaaa acttacttcc
     4741 tattctatca tcataactca ttcctaaagc tacatcttct atgttagctc ctgctatttc
     4801 acaaagattt gcaatatcat tcataaatga tattttaaga gctaagaagt tatttgatgc
     4861 gtattttatc atctcagcac tatttctatt tgtaactatt ataggttgat taaaaggctt
     4921 atagatttcc tctaatattt ctctagccca ttcactttct acacctataa cgattcttga
     4981 agcatgtaga gtatctttta ctgctgttcc ttgtgataaa aactctggat tagatgcaag
     5041 ttctactttt acatcatttt ttaggtttct atttatataa tcttctatct cttcatttgt
     5101 accaattgga actgtagatt ttattactac tagagtatct tttgtcacag tttcagctat
     5161 ttgctttgca actgccttta tataatctag gttggctgaa ccatcacttc tctcaggtgt
     5221 acctacagct attaatatta catctacatc ttcataagct tttttatagt ctgtagtgta
     5281 atctattctt cctctgttgt aatttttaac cataagttct tctaggcctt cttcatatat
     5341 aggtgctatt ccaagtttca ttgatttaac tttttgttca tctacatcaa cacagactac
     5401 attttcattg cctacctctg ctagacaaac acctgtaacc aaacctacat atcccgtccc
     5461 agctatagct attttcattt ttttacctcc aagtatataa aacttatact ctttttttaa
     5521 tggcattaaa aacatagaaa caaaatacat atagactttt tggtaaactt aaattttcaa
     5581 catttctata agtattccaa gttcgtttta atgcttttat cttgttactt gatatagaat
     5641 ttccaactaa tctgtaattc actaaatctt cttgaatccc atatgcatat ttttcctttt
     5701 taagtaactg taaccaggtt gcagtatctt gtcctcttct taccaaaggc atgctgaact
     5761 ctcctacaat ctccttatca atgaccactg tagaacatcc tattatagta tttctaagta
     5821 atccttcata gtcaattttc ttaggagctc ttgcaacctt attcgttttt gacccatctt
     5881 gtctcatata tctataagat gtaaaagaaa aacctacatt ttcttttaac atatattcta
     5941 tctgaatctc taattttcta ctatcccata aatcatcact atctacaaat gcaataaatc
     6001 gaccttccgc attcttaata ccaacatttc tagatactgc agctccagaa tttttttcaa
     6061 gctttatata ttttattcta gcatcttctt ttcgaaaact ttttataatt tcacttgaat
     6121 tgtccttaga acaatcatcc actaataaaa gctgccaatt tttataagtt tgattttgga
     6181 ttgactttat cgtctctgat aaaaactctt ctgaattata aacaggagta attatcgata
     6241 ctaaaggctc attcatattt ttactctcct tatatagcaa ctagcagtac tataccaaca
     6301 agtttactta gatactaatt ttctttgtcc aatatatttg taatatccta atgggaataa
     6361 tgctaaatac aacaatttat cagatacttt tcttaattca ccaaaataat gactatcaga
     6421 caacaatgag tatctggcta aatttatgta attttttatc cacatatatg gatatctact
     6481 taatggatat gtattttgat ttattaaata taaaaactct aatgctctac ctcttaatgc
     6541 atttttactt aatatattct tagtcaaacc atcatccaaa taatctctta ttatcatagg
     6601 tttatttaca catataactt tatagtattt agacatttca ttccatacta cactttctgg
     6661 taaaaatctc acttcttttc tttctggaaa aggaaatttt ataagctcat cagttacaaa
     6721 tactatgctt ttatctccct taacgccata cttaaaatat aggtctgcaa agtgacatat
     6781 tttcttatct tcaggcattt ctgtccctat caaatctcca cttggatata cacaaagtcc
     6841 aacaactcca ctaaatcctt ctttattctc taaagaatcc cactcatcat tagcaatttc
     6901 aagagaatct ttaattaaac catcatcact atctacaatg aaaaatagct ctccatctgc
     6961 attttttaca cctagattta ttgcagtatg ttttcctcca ttttctttgt atatatatct
     7021 tatatttaat ttattttctg atataaattt ttcaactaac tctttagtac tgtcgctact
     7081 tccatcatca acaatcaacc attcaaaatc attgaaatca taagtttgag cttttaaatc
     7141 attgtacaaa ttttcaagta aatgagctct gttatacgtc ggagtaaata cagtaaattt
     7201 atatttatac attaattaaa tcttcctctc tgtctaactt tttttgattc cgtttattcc
     7261 ataattaata aagtagtata tagctttgta taaaggtatt ttttctatat ttctatatat
     7321 attccaagtc cacatagcag cttttatttt attaccactt agggaattgc tagattttct
     7381 atataatgct agaacttcat ttattccatg agcaatatgc ccttttttca atatcgataa
     7441 ccataaaaca taatcttcat gtcttacacc actcattctt atttcaaaat ccaatttaga
     7501 cttatcaatc acaacagtaa gacatcctaa gatatttcct tttaataatc ttctataatc
     7561 tacattaggt ggaactttta ttactttgtt taatttttta tcattttcat ccatcagttc
     7621 atatgaagta aatgaaatta catagtcatt ttctaacata aaatttactt gtttttctaa
     7681 cttactacta ttccattggt catcactatc taaaaaagct ataaattgtc ctgttgcctt
     7741 acttagtgct aaatttctag cattagagac acccttatta gtctcagtct ttatacattt
     7801 tattctacta tcctgttgca tataagattt gactatatta ggactattat ctgttgagca
     7861 atcatcaata attaacattt cccattcttg ataagtttgg tttaatactg attttatggt
     7921 tgcttcaata aatttttcag aattatacat gggagtaatt atagatacta aattcttctt
     7981 catattacct tcctcatcat ttataaaata tttatttaat tttacttaat acacaatcat
     8041 atatcctctt gcagttctta ttatcattat atctgaaaaa cttatttctt tcattttttt
     8101 ggttatccat aacctcaaaa ttattttcaa taatatcaat aagtttattc acggcttcat
     8161 cattattata agccaaagac ccaaacattt cactttttaa atctatatat gaatcaactt
     8221 tttccatata ttcatccaaa tcgaattggt aaaacaagat aggtctattc atatacaaaa
     8281 agtctatggc tacacttgaa taatcagtta tatttgcagc tgattttctg atttcatttg
     8341 ctagagttac ccctttatct aaaaacacaa ttcgttttcc tgttatattt tcttttatat
     8401 catctataaa ctcatgcata agatgatgca tataaatttt tacatttata tctttctcat
     8461 ctaatatttt atttaatttg tcatcattta aaaaattcat tatattttta aaataatctg
     8521 tatccgtaaa ttttcctgaa ctaaatttta tccaatctct ccatgtagga gtatataata
     8581 tatctcgagt ttgtgcaact tcatttgttg gtaaattgtc atatcttgca tttccaatta
     8641 tataaactga gtcatctaac attccccaat catttaccat aacatttctt tcaaattcag
     8701 aacttgctgt aactatatta tagcttttat ataggttatc tataatattt ttgaactttt
     8761 tgttcatagg ttttcttttt ttaaaactta tagtcccatg atttaaaaac acttttatcg
     8821 tttttttatg aaaataatta agaacaggta cagcaaaatt gtatggtgct atatctgctg
     8881 atggtgcatg tgaaaatact atagcttttg aattgtaata atacagatag ttttctacac
     8941 ttcctcttat taattttttg cctggaattt tatcaaatac tttactgtct ttatttacta
     9001 cccaataagt ttctacatca ttatgttctt ttagcatata ctcataaaaa aactttgaat
     9061 tatcattgta tatatcccct gaatgtccac ctataagcca gatatctcta ccattaaact
     9121 tatgcttatt aaatggatat ataaaaaaag ccaaaagcat atttattaaa aatttaacct
     9181 tgtccataaa caccatcctc attataactt gtactgtcta aatccattaa ttcttccttt
     9241 tatagtggca ctatgataat ctctattgga actcaatata agacctccta tatttcttac
     9301 aagttttgat accataactt ttgcaaactt ataatttttt ctatagacta tttccttttt
     9361 gcaaagcgct ccaaagcctc ttccatagtt ataatctttt tgatactttt ctttagattt
     9421 agaatgtttt tttgcaggat ggtaaattat atcatttcca aagtattttc ctttaaaacc
     9481 taaactcaat aagtttaaaa cataatctat ctcttcacca gcaccaaact ctccaccaac
     9541 acccaatttt tcatcaaatc ttgtatagtc tttaccttca aaatcaataa aaaaagttat
     9601 ggatgttata gtatccaaaa cgttggaact tgtgatatca caagttccat catacatttt
     9661 cttaaatgca tcaactttat ttgaatccat agttttgcaa ctgtatattt tataatcttt
     9721 atttttatta aagaaattta taactttttc taatgtatca ttttcgtaga cacaatcatc
     9781 atcagggaaa cctataattt gccctacagc attgtctatg cctacatttc tgttataact
     9841 caatcctttt ttagagcttt ttatatactt tatatctaac ttatcaatgt atttatcaac
     9901 tatttcttta actttgctat tatcattttg gtctactact ataagttcaa agtttttgta
     9961 agtttgattt tctaagctgt ccataagtaa gtatatatca tcatatctat ttaatgttgg
    10021 cattattaat gatattaaca ttactacctc ctacttttca tcttccacaa atctatttcc
    10081 cttttatcat ttaatatcac ttcttttatt agtaatacta ctgcaaaact aaatgaagct
    10141 tctaataata aattttctgc aaaagagtat agtaaaaata taaataaagc tactaaatat
    10201 gcttttttat ctttttctat aaagctttct attcctttat acatcaaata cataaataat
    10261 atacaagcaa ctactcctag tacactaatt atatatacat aagaactatc taatggatta
    10321 gttgctttta tagcaggact atatgaattt ccaaaaggtt ttagaaagtt ccccttttca
    10381 gccaagtata cattccagaa cttaggtcta cttgctaata atctatttaa taaagtgctt
    10441 ttatcaaata ctgtaccaat aattacacta aataaagtaa gtattaccgg taatgtcttt
    10501 aggagcattg gtagtccttt tatttttttt atatctatcc atcttattat ttctacaaat
    10561 attaatcctg ctaatatagt gaaaaatcca gttctactat atgtggtttg ataaacaaaa
    10621 aatgcagaac caaataaaag tattctatcc catttattat aatctttata tcttaagaat
    10681 atatatgctg catatatagt aaccatatga taaaatacac tatttggatt accaagtcca
    10741 aaatccattc ttgtaacaag catatctatt ttttctatat atctatagtg gacttcctga
    10801 ttaaatatta atgaaggaaa taaattaaat aataatatag ctaccattaa aaaacatgcc
    10861 gatattatta aataattttt aactataaat ttatcatcta tttgcttagc tgatacagct
    10921 aataaaacga ttaataatat agctccttcc ctatttaata aataaagtaa gacacaaact
    10981 ccaactagta ccatttctgt tatacttttt ctcttatcaa ataaaaaaag tagcattagt
    11041 attcctattg aagaataaaa taacgctgat ttaatcatcc ctatatccat atttgggtca
    11101 ataaatgata tcatatactt tctaagtaat atgataaaca aaacaaatat ataaagatat
    11161 tttctaatca taatatttct atcatcattt aaagtcttca ttttactttc tcccttttag
    11221 cttcgtaaag tattacttgc aaatatttta caacacttcc atatcttatt tacacctgtt
    11281 aagtgtttgc tttctaagaa ataatttctc tttttagccc aactttttcc ccaccaatgt
    11341 atcccaaaag tgttttctgt tatacattct ggtgtaaagt cttctttaaa tccaaaagga
    11401 taaaaatatt ctttgtcgta aacacgtatt ccttcttcat aattatttgg atcaatcttc
    11461 ttataattct tttccataag atgagtcatt atctttggta ttgtaaacaa tgaagaagta
    11521 agtacttctt tttcataata ctccaataag tcttttaaaa aaggatgtcc tttttctaca
    11581 cctattatag caccatttat atactctcta tcatcttcat acccacagac tagcctatta
    11641 ttttctaaaa gaggagttat atcctttaat atttgcatat ctgtatccat ataaactcca
    11701 ccttgttcat agagtacttt tattcttgta taatcagata tgaatgccca aagttttctt
    11761 ttataacatt cttccaaaaa cttatttcct tttatttctt tctctatatc aaagtttttt
    11821 tcattccact ctactatctc atattctgga agcttttctt tccaagaatt tatacatata
    11881 ttttctatat ttccttttgg accaccaaac catacataat gtattttttt aggaatcaaa
    11941 tgaacacctc ctacatatat tcttgtctat cttcttcttt taactgctct actatttttt
    12001 tgacatcttg agctttatct tttctacaaa ccataactgc atcatttgta gatactacta
    12061 tcaaatcact aatacctatt gttgatatta atttgtcatc tgaatatata attgaatttt
    12121 tagtatctat agtaatattt tcacctcttt tgatattacc ttcatcatca gttggatata
    12181 ttgcacctag agaatcccaa gaaccaacat cattccatcc aaaatcacca ggaactacta
    12241 taacatcatt acttctttcc ataataccat aatctataga gattgattgt atagttgggt
    12301 atatttcttt tattttttcc atttcttctt ttgttcctaa gtatttactt atatcttcta
    12361 atttttcata tacttttggc aagtatctct taaaatcttc tagtatttta gataccttcc
    12421 atacgaacat tccactattc catacatact ttttagagtt tacatattct ttagcaattt
    12481 catagtttgg tttttctaca aactccacta cctcatatgc aacatcttct atagtatttt
    12541 ctctattaaa atttatgtat ccatatcctg ttgatggaaa tgtaggagtt atcccaatag
    12601 ttactagttt atcattattt tctgctatat agatagcttt ttccaaaata gatttaaatt
    12661 ctttttcatc ttttatataa tggtcagctg gatatacaca cataacacca tcaccatatt
    12721 ttttcattat gttaaatgct gcaaagccta tagctgctgc tgtatttctg gcacatggct
    12781 ctggcaatat attactatcc aaacatttat cttttactat atcctttaaa gcttccaatt
    12841 gcttctcatt ggttactata aataaatcat ctttttttgc tagagaatct attctgttta
    12901 tagtttcgtt tattaaagca tcttctccac ttaaatttat aagctgttta ggtacttctt
    12961 gtctactaag tggccagaaa cgagttcctc ctcctccagc cattatgaca ttgtatactt
    13021 tcattgttta tctcccctta gctttttata tagaatccac tacatttaat atataagata
    13081 ttaaattgtt caacttaaat tctacatctt cttgattgtc tgaatttgct ccaaagtaaa
    13141 atttaatttt aggttctgta cctgaaggtc ttatagctat ccaagagcca tcttcaagta
    13201 aaaatttaag tacatctgac ttaggtaaat catcaacacc atctttatag tctaaaattt
    13261 tatctacttt tacatcagcc acattatcta ttttaacact tctaaagtaa agcatcatct
    13321 cttttatttt ttttattcca tctatgcctt ttaaagttac tgactttaag tcctctttgt
    13381 agtatccaaa tttattatat gtatctatca aagcttcata caagttcata ccttttgaag
    13441 aatagtatgc tgccatttca catatcataa gtgaagatac aacaccatcc ttatctctag
    13501 catgagttcc aattaagtaa ccatagcttt cctcatatcc cattataaaa cttctatcat
    13561 tgctttcttc aaacgctttt attttttctc ctataaattt aaatcctgtt aaagtattta
    13621 aacaatctac attataagcc tttgctattt ttgcacctag ttctgatgta actatagttt
    13681 ttattattgt aggattgttt ttaggaagtt tattttcttc aactaaactc tctaatatat
    13741 atctaactaa aagtgaaccg acttgatttc catttaagac tacatattca ccacttgaat
    13801 cttttacaac gatacctact ctatcacaat cagggtctgt tcctattatc aaatcagtgc
    13861 cattattctt tgccatttca atagctatat taaacactga cttttcttca ggattaggat
    13921 actttactgt tgaaaaatta gaatctggca attcttgctc cttaactacc attacatttt
    13981 caaaaccaca ttcatttaag acttttctta ctggcttatt tcctgtacca tgaataggtg
    14041 tatatattat ttttaatttt ttcccgtatt catctattat attttgtctt aaaacttgtt
    14101 tcttaacagc ttttataaac tcatcatcta cttcattatc taatatagtt attaaatttt
    14161 tagataaagc ttcttcaaaa tctatacttt taatagtact gtaatcatca atcttattaa
    14221 cttctgctat tatatcattt gccatatcta tacagatttg accaccatct gagtcatata
    14281 ctttatatcc attgtattct ttaggattat gacttgctgt tattactatt cccatagcac
    14341 actttaaata tctaacagca aaagaaagtt ctggtgtagg ctttaattct tcaaaaatat
    14401 aagcctttac accataagca cttaaagtct ttgctacttc tatacaaaac tccctagaca
    14461 tgtatctact atcatgtgct ataactattc ccttttgctt accttcttct ccttgagttt
    14521 tcatgatata atttaaaacg cccagtgtcg cacgtctaac agtataaata ttgattctat
    14581 tagttcccgc tcctattata cctcttaaac cacctgttcc aaactctaaa tttttataaa
    14641 atctatcttg tatttctttt tcatcatctt ttatacttaa tagctcattt ttcgtttgtt
    14701 catcaaaata aggagaattt aaccacatct cataattatt cttataatcc atattttcat
    14761 cctttctgac tctaatttaa aatttcaagg taattatcta tcttctaagt agtttttgtt
    14821 ttccttttct taaaaattca aaagctaaat ccatctcttc taccttaaat aaaactatca
    14881 atacagtata tacagaagct ccacctaata ctccagctcc aactgatata atttctttaa
    14941 ttgttcctga acccataaat gcatatagat tattatatat caataaagta gctactgcca
    15001 tcacaccaga tgctaccaaa ctttttagac cagtctttat aattttatcc cctccaaaat
    15061 atccattttt ctttttaaga gatataaata ataatataac tgtaattatg tttgatgtac
    15121 tagttgatat agcaatacct gcatgaccca atggtcttat caatattaaa ttaagaacta
    15181 tatttattat taaagctata cttccattta tcattggtgt ttttgtatct gaaagagagt
    15241 aaaatgccct atataagaca tctcttactc cacaagccaa taaacctagt gaatataatc
    15301 taagcgctat agatgtaagg tctgttgata acgcatcaaa tttacccctt tggaataata
    15361 ttctaacaag aggctctgct aatataatag ctcctataga tataggaagc accaccaata
    15421 taatacaatt tgaagatttt acaatagtgc ttataaatcc ttctttatta tctttaccag
    15481 acatttttgc tagtttagga tatataactg ttattataga tgtaacaaat aatgccataa
    15541 caaactcatt tagcctattt gcatagttaa gagcagacaa ctttccatca cccaaagtgg
    15601 ttgccaataa tcggtcaaca aatatattta actgtccaac agctactcct accaacatag
    15661 gcaatactag gttgactaac tcttttatag actcatcctt taaatttata tatggcttat
    15721 acttgtagga cttcttaaac gcaaatggca gttgaaacaa tagctgactc gccatagcca
    15781 ataatgctcc tgctggcagt atccatacat ttttacctgc acttagtgct attgctgcta
    15841 ttattataat attatatggg attcctataa gcccaggaat tacaaaactg tcattcacct
    15901 gtaaaaacga actaaatatc ttacttccac tcaggaataa aactcctgaa atcattattt
    15961 tagtaaataa tattgctgct tggaacttag cagggtcgtt tctaaatcct gctgcaaata
    16021 ctgtaacaaa ttgctctgta aataaaagac caataatagc tattactata gccattatat
    16081 agcatatatt taaaacatta ttagtaaatt ttaaggcacc ttcatctccg agtcttttct
    16141 ttgtctcaaa gtacataggt atataagttg tagcaactgc agagcctatg actgctatga
    16201 ttaaaccagg tatatttaat gttaatacaa atacgtctgc atacatacct gtcccataga
    16261 aatttgcaag tacaagttct ctaaggaaac ctaatacttt tgaaaacata gtggctgcca
    16321 taatccacaa tgctgcttta gctgtttttg acatcttctt tctccttatc taatttaaac
    16381 tctaaaagta gaacttttaa aactgcttat aaacattatt ctaattaagt aaatttaacc
    16441 tatgaaaagc ttccttataa atcaaaaact atttcgactc atatctaagt gccctaaatg
    16501 taatcaatag taataatagt attaatacta tacaagataa gaatgttaga ttaaatatgc
    16561 tcttaaatag actataatca cctactgtat acccaacttc aactacatca tttatattgc
    16621 tatctttcaa gaatttatct tgtgcacgac ttaagttatc actacaaatc agtattggca
    16681 tattttcttt tgctgatact actcccaaag aaatatattc gtttacatca gatctactac
    16741 caaagtctaa tctagcagta tagattttct ttatatcttt ttcatcataa aacttcttta
    16801 ttaaatcaac atttgtactt gacttattta tttgctttat tccttctata ttttttaagt
    16861 tagaatcaat ctctgcaata aactcttttg aatcacctaa tgcatatatt tttttaagat
    16921 ttttgccttt taagaaatca atttgttcct caaacccttc atctttagcc catattattg
    16981 gcattccact tttagcggca taagaatata cagatatggc gttctctaat cctgttgttg
    17041 agctaagtaa aaatgcttca tcaaatccta ctgttctata catttctcta gctatcttta
    17101 aagatgtatc atatctgtct ttccctctta ttctttctac tttcattccc attttttcta
    17161 tattacgttc agcatcttta gatactgcct ttaaaccacc tactatcgta accttctctg
    17221 gacctagttc ttttagataa tttcttgtta ctctaccaag atttttactc ttagttacaa
    17281 ctattggagc attttttgca tatgctaaag gtgttgcact tatactatct acaattgact
    17341 cttcatttac aagaattact tctttagaat ccttaaatgc tcttttattt aattctgtcg
    17401 aaatctctgg tatactccct tctatcaatt ctcttttggg atttgcatct gcaaatactc
    17461 ctatcgtact agaaatagag attaacaaaa ctaacgataa tatagtagaa atttttttaa
    17521 tcataagttc tcattccttt tcttttttgt attttttgac aataaaaaag ctctttttaa
    17581 agagcttttt cattgattag taatctaata ttttaaaatt atctagctcc gtctcctgtc
    17641 aatactactt taacagtttt aaagagtatc ttaaagtcaa gtaatatact cctatttttt
    17701 atatagtaaa tatcttcttt aagtttttct tcaggagtga tttcgtatcc accatttact
    17761 tgtgcccaac ctgtaagccc aggttttata gcgagcctgt tgataaatcc aggtatttcc
    17821 tcattaaatt gaacagtaaa gttcggtctc tctggtctag gacctattaa gcccatgtcc
    17881 ccctttaata tattaaacag ctgtggaatc tcatcaattc tagttttcct aataaacttt
    17941 ccaaccttag ttattctagg atcatctttt tcagcccatt gtgcgccaaa tttttctgcg
    18001 tctgttctca tagatctcaa tttatatata tagaatcttc tcccgcattt tcctagtctt
    18061 tcttgcttat aagttattgg tcctttgtcc tctattttaa taagaattcc aaatattgct
    18121 attaaaggta aacctatcac aagtcctatt atagataaaa ctaaatccat aattctttgg
    18181 tacaagtcga ataaaactgt tccttttact atgctatagt caatctcact tgcaatttct
    18241 ttatctgccc ctggatagct tttaatttta ggtatttcac actcatcatt agtatatcca
    18301 gacatagtta cctccatatt ggaataaaaa tttttttaat tcttaatttc aacttatttc
    18361 tatcttattt ttgacttatt tctatattat actcaataat gagcagatta tcaatacaag
    18421 aaacatcatt gtaaacaatt tgattcttta caaaaatata tcatctcttg ttatatttta
    18481 cattataaca ggagatgata tgttttgctt taatattttc tattttattt tactgtatct
    18541 tctattccta ttccaatttg agttcccatt gtttttactt tttctgcagt atttaactta
    18601 tccatatcgc tcttattgtc aataaatgca cattcaacaa gtattgctgg ataattgtta
    18661 tttcttaaca catataggta atcttttcca ttatcaagtg ttcttgtctt gattcctcta
    18721 tttttcatgt taaatttttc tagtattctt tttaagatat ttgatgctgc agtcttagtt
    18781 gttccaccat ttttatcttt tactttgtaa taaatttcca caccattacc agctccatta
    18841 gacgcattgt aatgtatact tgtaaataaa tctggcttta tagtatttga taatgctgtt
    18901 ctttctccta aagccattgt tttatctgta tctctagtca taacaacatt tatacctttt
    18961 gaacgtaaat attcagtagt tgctaatgct gtattaagag tatatttctt ttcttgtgct
    19021 ccaccattta atccacttgt tgcccctgaa tcagaacccc catgacctgg gtctatcact
    19081 acagtttttc ctgctgctga acctgaatta tctggttctg ttggtgcatt gtgttttgat
    19141 aaactagatg ctatagaatt tataacacta tctttcattc catcaccaat ttgatatact
    19201 gtttctgctg ttttttctga taaatttgta ttatggtatg cagaaacata agtttttgga
    19261 gttataagta ttggagcttt taatttagct gctaaaggtc ctgctgttat tgaatcaact
    19321 attatgtcag attttgcaac taacattgca ctcaactctt tatctggata gaaagtctta
    19381 attacatttg catttgtttc atgtctatct gctccagaaa ctctattttt tgaagtacca
    19441 ttttttgcta ttttagatat ttggtctata atttttgatg ataaactttg actaccacct
    19501 atgtaataag cttcttctaa accttgtgaa gataaccaat tatatgtacc ttgtggtaca
    19561 gaatctttat tagctaatat tataggttgt tggtcttcac cagcttttga tgaaatatta
    19621 agagcatcat attcaccagc atatccattt gctatgtata ttttattaac atcatgatac
    19681 ttatctattt cttttgctat aagtaaagat gtttcatgtc tatcaactcc accaatacga
    19741 gtcacattta catttacagc agatttaatt tgagacactg ccttttgtga aactgaacca
    19801 tcatcaccta taattattac atcacttgga tttagacgtt ttaactctgc ttttgtactt
    19861 tctggtatat catctttatt tgcaagtaat attggtgcgt catatgtaga tgcaagtgga
    19921 gtagctgtaa ttccatctgc tgctaattct ccattaacta ttacaacctt actagaccca
    19981 tctttccaac cttgtttact tatctcaaca gcagtttcaa atctagtagc tccagcaagt
    20041 ttttttactg gtgcagaagt ttttctcata tattcattta ctgcttttgt tgaatttgca
    20101 tcacttatta taaactcttg tgtaacatca taatccttaa gatttaatcc catagactct
    20161 aagtcttctt tgtttaaaac tttctgttca tctttaccat ttatttttat gctttgaagt
    20221 tcccatacat ttaagtattc tgggtctttg ttatagttta ttacaccttc atatgaatct
    20281 gtcttttcat tgtaatttaa agtagcttca agtggcatat cataacacat aaaatttaat
    20341 gttattgaat cagcatttaa tttttcttta aattttacac ttgcattaga tttacttcct
    20401 aacactacac tttttctttg taaatttata ttaatatctg aattagattc tgtatttgca
    20461 gtagtatttt cagccgctaa agctacattt ggtgccacca ttgctacaga caacattcct
    20521 gttgccaata attttgttgt ttttttcatc atatcccccc taattttttg gttctttaaa
    20581 tctttaaatc ttttttaata catcttaagc ttgttttata ttacataaat tcaatattat
    20641 gtaaatcttt tttcattata gcacaaaaaa ttacaaattg taaacttttt gtaatctttt
    20701 atggcatggt ttttaaattt tatcgaggag aaattttgat tatatttgat agtttctgta
    20761 tatttgaaca aatcaaagac tttatcatct aaaaatattg cactaaattt taataaatat
    20821 ttttttcttg tacattatag acattactat aacccagaat aggacataca ataaagaaaa
    20881 tataagagaa tcccaagttg tacctgccca aggtgttata agttcatatg tagtccactc
    20941 taccaaggta gttggataat cgactttgtt cgtaagtttt ggaacattcc aaaaaacatg
    21001 actcaatatc tcaaggcaca tatatgtaaa aatggggcta cttccaagtg ctattattgg
    21061 ggtaaatatt ttacttttat ttttgatgtc gcatataaaa taaaatattg acagtaatat
    21121 accataactg ccagccatta aaagtacaaa tgagctagac cataatcttt tattaaatgg
    21181 aaaatactga ttaaagataa aagcacctat tagcaggatt atgctcataa ccagtatttt
    21241 aaagaatttt ttatattctc ctaggttttc ttttaaaagt acacatccca tagtgcatcc
    21301 caacatacct gaagaaattg ctactatact agttaaaaaa ccatctggtt caaattccgg
    21361 cttgtacaaa tgtcctttga agaaatgtaa atccactaac tgtgcaagag aaccttctag
    21421 ctcaaatcca taaggttttg ctaagatgta gtacccaact attataaatg tagctataca
    21481 tagaaaagta attattgtag cagtacttcc aacatttaat ttttttagca gtaaatatgc
    21541 taaactagtc acaaaataga ctaatcccat acgttgaagt actccaagta ttcttacagt
    21601 atctaaatca ggatttccta ggtaatttaa gaaaaatcca aataatatca ataagatact
    21661 tctcttgaat atactaagta ttatactcag tgtggattta ttatttttta gtttactatt
    21721 tattgatatt ggaattgtaa cacctaaaga tataacaaaa aaaggaaatg caaaatctgc
    21781 aagtgttaca ccatgccata cagcatgtct taattgagga tacattctca tccaagtccc
    21841 aggattattg cagactatca tgagggctat tgatagccct cttattatat ctattgattt
    21901 aatcctacta ttcataagtt tagaatctac agcattattt gaaatt
//
LOCUS       DAMKMI010000058        21283 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMKMI010000058 DAMKMI010000000
VERSION     DAMKMI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.059"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            252..773
                     /locus_tag="PC429_001989"
     CDS             252..773
                     /locus_tag="PC429_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847494.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
     gene            807..1583
                     /locus_tag="PC429_001990"
     CDS             807..1583
                     /locus_tag="PC429_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3847495.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            1925..2878
                     /locus_tag="PC429_001991"
     CDS             1925..2878
                     /locus_tag="PC429_001991"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF3847496.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            2896..3915
                     /locus_tag="PC429_001992"
     CDS             2896..3915
                     /locus_tag="PC429_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF3847497.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            3946..4197
                     /locus_tag="PC429_001993"
     CDS             3946..4197
                     /locus_tag="PC429_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847498.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            4479..6284
                     /gene="lepA"
                     /locus_tag="PC429_001994"
     CDS             4479..6284
                     /gene="lepA"
                     /locus_tag="PC429_001994"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF3847499.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            6433..7770
                     /locus_tag="PC429_001995"
     CDS             6433..7770
                     /locus_tag="PC429_001995"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF3847500.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYTF"
     gene            8003..9316
                     /locus_tag="PC429_001996"
     CDS             8003..9316
                     /locus_tag="PC429_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3847501.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            9488..10663
                     /locus_tag="PC429_001997"
     CDS             9488..10663
                     /locus_tag="PC429_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF3847502.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            10766..11785
                     /gene="hrcA"
                     /locus_tag="PC429_001998"
     CDS             10766..11785
                     /gene="hrcA"
                     /locus_tag="PC429_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF3847503.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            11815..12435
                     /gene="grpE"
                     /locus_tag="PC429_001999"
     CDS             11815..12435
                     /gene="grpE"
                     /locus_tag="PC429_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF3847504.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            12527..14374
                     /gene="dnaK"
                     /locus_tag="PC429_002000"
     CDS             12527..14374
                     /gene="dnaK"
                     /locus_tag="PC429_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF3847505.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSESEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            14585..15739
                     /gene="dnaJ"
                     /locus_tag="PC429_002001"
     CDS             14585..15739
                     /gene="dnaJ"
                     /locus_tag="PC429_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF3847506.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            16705..17640
                     /locus_tag="PC429_002002"
     CDS             16705..17640
                     /locus_tag="PC429_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF3847507.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            17676..19049
                     /locus_tag="PC429_002003"
     CDS             17676..19049
                     /locus_tag="PC429_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3847508.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(19237..20004)
                     /locus_tag="PC429_002004"
     CDS             complement(19237..20004)
                     /locus_tag="PC429_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847509.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(20034..21143)
                     /gene="ugpC"
                     /locus_tag="PC429_002005"
     CDS             complement(20034..21143)
                     /gene="ugpC"
                     /locus_tag="PC429_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF3847510.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         8310 a         2392 c         3751 g         6830 t
ORIGIN      
        1 tataaattta tatgcttaat taagataatt tttccaataa tcaagataat ttttttcatt
       61 agtatgtatg atagatgcat caaacaatat aactaatatg ttagaaggtt atatgtaatt
      121 aatgtcagtg tttatataga atagcatttg tagcatttat tatagttgta attactattt
      181 atatcataga cttgaatagt ctaaaaaaat agactataat atttttatag actaaagaaa
      241 gggctgataa gatggacata gcaaaactaa ttttaaatcc tgcaaggctt cgtattttac
      301 aatatattcg actttatggc agtgttcgta catctgatat tgtaaagtat ttaaatgata
      361 ttcctcgtgc aacagtttat caccatgtaa aaattttaga agagaacaat atgattgaag
      421 taattaaaga aaatcgtgtg cgtggtacta ttgaaaaggt ttatgcacta aaggaatata
      481 caacatctat ggaaggagag accttcgttg cactttctac agcattttat gtaggtctga
      541 tgcaggaaat gaatgaatac tttagcagaa aagagcaaga ccataaaaag gataatgtat
      601 ttttctcttc agcattacta tatatgagtg ataatgaata tgaaaatctt cttaaaagta
      661 tagctgattt attgaaacca tatatagaac aaaaacctaa atcaaatcta aaacttagaa
      721 aactttcaat tatatcttct ccacctgtag ctgataatga taaaacaaaa taacatgcaa
      781 cccattttta taaaaggagg ttatatatga aatttcatga gtttggaaat aaaaacagac
      841 cacatattat gttgattcat gggggaggga atgcatggtg gaactatttg cgacaagcag
      901 aagttttgtc taacaaatat catgtgatac ttcctacatt ggatggtcat ggagaagaat
      961 atgaaacaac atacatttct acagaggata cagcagataa gttgatatca tatatagatg
     1021 aaaattgcag tggacatctt ttcgctttgt gtggggtatc acttggtggt caaattgtca
     1081 tggaactttt atctcgaaaa cctaatatta ctaaaaaagc aattattgat ggtagtattt
     1141 gctacccaag acctcttatg gcaaggtttt gtattgctgg tattcgattt tttggaggac
     1201 ttttgtttag tgagaaggct tgtcgctttc aaattgctat gatgccaaaa ttgttacctg
     1261 aaaatatgca atatccagat gaaataaaag cgtattatat gacagatatg ccacatgtaa
     1321 gaaaagaaac attctataat atgtatcgta cttacatgat gaactataca cttaaagaga
     1381 atgttaaaaa aaccacagca caagtaatgt actggtatgg agaaaaagaa atgaagtgtg
     1441 tcaaaaaatc tgcaaaactg tttcagtctt atgtgccctc ttgtgagatt tatgaagcta
     1501 agagctataa tcatgggtat ttatcacttt atcttccaaa tgaatggttg gaaattgcag
     1561 aaccattttt ccaaaaggaa taaaggtgtt aggtgattat tgtataatta ttgctgtttt
     1621 tttatatagt aaataaatta ggtataaata taaaaaatta ttttagttaa tatattagtg
     1681 aaagcttaat aggaaaatta taaatagatt aaaggtagca cctatataaa tagttgctac
     1741 ctttttctgt ttgtaaataa atgatagata agaaggttta ctgacaaatc actgactaaa
     1801 cccaattttc ttacattgaa agccttaatt ttacttgttt taaataaatt tacaatttct
     1861 tgttataaaa tactaaattt tgttaacaat tataattagc atttaattta tactggaggt
     1921 ttatatgatt agtgtaagaa cagatttagc tttagaagct agtgaaatgt gtgaaaagtc
     1981 tcaagaagga agtagtattc ctggtgtaaa aatagaaact aaagaattag aaaattgcat
     2041 tgtaactaaa gttgaagtaa tagacaggca aggcgcagag ataatgaata aagatatagg
     2101 aaagtatata actctggaaa gtaatcttat gaagtttgat gatgatgagt caagagaaga
     2161 aatgataagt tatttaaaag aggagttagt agatatattt ggccaagaca aaaataaaaa
     2221 aaccctggta attggtcttg gaaatagaaa tattacttct gatgcgttag gaccaaagag
     2281 tgtatcaaaa acacttgtaa ctagacatct ttttaaaaat tataataaag actatgatga
     2341 tgatttcact gaggtatcag ctttaagccc aggagttatg ggtgttactg gaatagaaac
     2401 tagtgaaata gtaaaatcat tggttgagaa ggttaagcca gatagagtag tagcaataga
     2461 tgcattggct tcaagaaaaa tggagagagt aaactctact atacagatat caacagcagg
     2521 aatatcacct ggtggaggtg ttggcaatac tagaaaatct ctaactaaag aaactttagg
     2581 cgtggatgtt attgcaatag gcgttccaac ggtggttgat gcagcaactc ttacaataga
     2641 tgtcttagat atggcaatag acaatttaat agctcaatca gaagaaacag aaagttttta
     2701 tgaaatgttg aaaaaactta aagaagaaga aaaatatcat ctaataaaag attcgttaga
     2761 cccatatgac aagaacttaa tagtaacacc aaaggatata gatgatacaa tcgaaaatct
     2821 atctataata ataagtgaag gcttaaatag gtctttacat ccaggtagat tggtataaaa
     2881 taaggaggag caattatgtt taaaaaatgc attaaagtag taaccttgac ttttatatta
     2941 gtatgcattt taccaggaaa atcattagct ttgaatcaag atgatttttt aaagttttta
     3001 gtaaattcat cttatccaga agctaaagtc gaaggaaatg atacagaaaa taaaaagaat
     3061 aataaaaata aagaaacaag taaggaaaat aaggaagaaa gtaaagaagg aaacactaaa
     3121 tctaaagatg caagtaaagt tgataataaa aaagagagtg aaaaagaata tataaaactt
     3181 tatgttggta aagaaaatgt tccagatatt gagtcaaaaa attcagacac aactgaaact
     3241 aacacaacat ctagttcaga ttataaggat gatttaagag taaccaaaga aaatccaaga
     3301 attttgatat atcatactca tggatgtgag acttattcaa attcacctga tggaaattat
     3361 cattcaagag ataagaaaaa ttcagtaatg gaagtaggaa gtgcattaac tagtgcttta
     3421 gatagcaaag gatggggtgt agtccatact actaaatacc atgactatcc atcttataat
     3481 aactcttatg caagtagctt aaaaacaata caaagtatat tacctaaata taactcagtg
     3541 gatatagcga tagacttaca tagagatgct agggatttaa ctaatccagc cactaaggaa
     3601 aaagaccatt taaaatatac aactatgata aatggagaga gagtatcaaa gttcttcttt
     3661 gttgtaggag gtaaaaatac aaatagaaaa caacttagag ctttagcaga agatataact
     3721 gcttttgctg agaaaaagta tcctggtcta gtatctccaa tagtagaaaa agactatgct
     3781 agattcaatc agtttgcagt taagaatcat atgttagtag aaatagggaa taacgcaaca
     3841 agtgtagaag aatctaaggc tactacaaag tatttagccg agattttgga tgaatatttt
     3901 aaacaaaaaa attagagctt taagttataa ttaggagcat gctaaatgag ccgattagaa
     3961 aaaaatatag aaagtaaaag taaactaaga aaatacaagt atattatgag aatcttattt
     4021 ttgattacta tgattatatg tacatcagtt tgtgtattta tagtggacag taatgcaaaa
     4081 agtatgctag gaatgaaaca atatgatttt ccaagtatta aatttgatat aacaaaattt
     4141 gattttgaca taccaaattt tgatatacat gaaatcataa aaaaatttaa taagtagtaa
     4201 taagtgaata aggtttattg attaaaataa gccttatttt agttatatat ttttaatgta
     4261 taaaataaaa attcattaac tgtattttat aggatttact ttaagtttat gatattatat
     4321 aatatttagt gttagacttg ataatccaca tataatgtgg ataattttgt ttagtgccaa
     4381 ttagtttata ttcaaaaatt ttaaatttgg tatataaaga gtttttagat aatgtaaaat
     4441 aataactata agaataatca agaggaggga attttaaggt ggacaataaa caaagtagaa
     4501 ctagaaattt tagtataatt gcacatatag accatggaaa gtctacctta gctgatagat
     4561 taatacaaca aactgggctt gttagtgaaa gagacatgaa aagtcaatta cttgacaata
     4621 tggaccttga aagagaaaga ggaatcacta taaagcttca aaacataaga ttaatgtata
     4681 aagcgaagga tggaaatgaa tattatttaa atcttataga tacacctgga catgtagact
     4741 ttaactatga ggtatcaaga agtttagctg catgtgaagg agcactatta gtagttgatg
     4801 cagctcaagg tgttgaggca caaactttag ccaatgttta tcttgctata gaccaagatt
     4861 tagaaatatt accaataata aataaaatag atttaccaag tgcaagacca gaggaagtaa
     4921 aaaatgaaat agaagattta atagggcttg attcaagtga agctcctctt atttcagcta
     4981 aaactggttt aaacatagaa gatgttttag aagatattgt aaaaaatgtt cctccaccta
     5041 aaggagataa tgaagctcca ttaaaggcat taatattcga ttcttactat gatgcttaca
     5101 aaggtgttgt agcttatgtt agagtatttg aaggaactgt taaaaagggt atgacaataa
     5161 aaatgatgaa taccaataaa aagtttgagg taactgaagt tggtgttatg gctccaggtc
     5221 aaactgaact tagtgaatta tcagcaggtg atgttggtta tattgctgct agtataaaag
     5281 atataagaag ttgtcgtgtt ggtgatacaa taacagattc aaataatcct acagaagaac
     5341 ctttaccagg atacaaaaaa gctactccaa tggtatattg tggtatatat ccaggagaag
     5401 gagaaaagta tgagaatgtt agagatgctc ttgaaaagct tcaagtaaat gatgctgcac
     5461 ttgaatatga agctgagact tcagcagcac taggatttgg ttttagatgt ggtttcttag
     5521 gtcttttaca tatggagata atgcaagaaa gacttgagag agaatttaac cttgatataa
     5581 ttactacagc tccatctgtt atatatagag ttacaaaaat ggatggagag gttgtaatga
     5641 tacaaaatcc tgcaaactta ccagaaccat ctgagataaa gatgatagaa gagcctatag
     5701 taaaaggaga tataatagtt cctaaagact atgtgggtgt tgttatggag ctttgccagg
     5761 aaagacgtgg aaatatgtta aatatggaat atatagatga tagaagagta atgcttcact
     5821 atgaccttcc tctcaatgaa gttgtttacg atttctttga tgctttaaaa tcaagaacta
     5881 gaggttatgg ttctcttgat tatgaggtta aaggttatgt agcatctaca cttgtaaaac
     5941 ttgatatatt aatcaataaa gagcaagtgg atgcacttag ttttattgtc catgagacac
     6001 gtgctttccc tagaggaaag gcaatgtgtg agaaattaaa aggagaaatt ccaagacatc
     6061 agtttgctat accaatacaa gctgctgttg gtaataaggt tattgcaaga gaaacaataa
     6121 gcgctctaag aaaagatgtt cttgctaaat gttatggtgg agatatatct cgtaagaaga
     6181 aacttcttga aaaacaaaaa gaaggtaaga agcgtatgag acaaattggt tctgttgaag
     6241 ttcctcaaaa agcatttatg tctgtgctta aattagatga gtaaatcctt ataaatttag
     6301 ataaagatat ttaaaagcta gagtgatttt agaattattt ctaaattagt ctagcttttt
     6361 tatggtatat tttaagtaaa ctcatagaga atataagtaa gaatattaaa gaagaataaa
     6421 aaggtgatat ttatgattaa tatagaaatt cctaaaaaag tagactatat aattaaagaa
     6481 ttagaaaaga atggttatga agcttatata gttggaggat gtgttagaga ttgtttatta
     6541 gaaagagttc caaacgattg ggatataaca actagtgcta gaccagaagt ggtagtagaa
     6601 ttatttgaaa aaacaattcc aactggtata caacatggga ctgtaacagt tatgattgaa
     6661 catgaaccat ttgaagtgac gacttataga attgatggaa attatagtga tggtagacat
     6721 ccagattcaa tagaatttac aaataacata gttaaggact tgtcaagaag agattttact
     6781 atcaattcaa ttgcatacaa ttcaaagaca ggattagtcg acccatttaa tggctatgaa
     6841 gatatacaaa ataagtatat acgttgtgtt ggaaatccag tagatagatt tgaagaagat
     6901 gcacttagaa tgcttagagc agttagattt tcagcacaac tcaattttaa aatagctgaa
     6961 ggtactaagc aaagtattca taaaaaagca gatttgataa aaaatgtttc tattgaaaga
     7021 atccagacag agtttaataa aatattagta tctgattcat ctaaattaaa cttattaaaa
     7081 tcaacaggtt tgttgaaatt tataattcct gaaatatgtg aattagaaga tgttactcaa
     7141 cacaatccat atcacatcta tgatgtacaa aaacatactt taatagctac agaagtaatt
     7201 gaagatgaac tgtatttaaa acttacaatg ttgtttcatg atttaggtaa aaaagtaact
     7261 aaaacaactg ataaaaatgg tgttgaccat ttttatactc atagtagaga atctgtaaag
     7321 attgcaaaga agattttgaa aagacttaag tatgataatt atactattaa taaagtttta
     7381 atattaatac aatatcatga ttatagaata gaacctaaaa ggaagataat taaaaagtta
     7441 ttaaataaac ttgaagatgt tgaattattt gaagatttaa taaaagttaa ttgggctgat
     7501 acacttgcaa aaaatccaaa gtatgcaaaa caaaagatat taaatcttat tgagtgtgaa
     7561 aaagaattta aacatataat aaatcaaaag gagtgcttta atcttaaaga tttagctatt
     7621 aatggaaaag atttgatatc tataggtata aaaccaggta aagatatagg atatatttta
     7681 aataagatgc ttgaaatagt cataaataat ccagagttaa atgaaaaaga aatactaaaa
     7741 gaaaaagcat taaatatata tacattttag attcaaatat aaaaaattta attactttaa
     7801 aaaacataga ataaaatact tatttagata aaataaataa tttttagaga atattttaga
     7861 agttacgatt gtatgatttt taaatgcata ggcatttact ctaggactat agactaatat
     7921 aaaaaaaaac tttgggaata ctaaaaccat aaatttataa aattaatatt ttatatactg
     7981 taaaacagtg aggagggaca gtatgcatac tcaactacaa aagactattg gtctttcagc
     8041 agctctttct actgttgtag gtatggttat aggttcggga gtttttttta agccacaagc
     8101 aatatacact acaactaatg gagctccagg tctaggtatt atagcgtggc ttttaggtgg
     8161 ttttataaca ataactgctg ggcttacagc tacagaaatt tcagctgcta taccaaaaac
     8221 tggtggtatg atgatttata tcgaagaaat atatggagaa aagttaggtt ttttaactgg
     8281 atggatgcaa actgtattat ttttcccagg tacatcagcg gcattaggag taatatttgc
     8341 tcaacaagca tctgaacttt taggaatgag cccaaataat atggcaaatg tacttcctat
     8401 agcaattgga gttatactat ttttagcgtt attaaatata ataggttcat ccttaggagg
     8461 aaaagttcag acagtagcca ctattggaaa gatgatacct ttaattttaa taatagtctt
     8521 tggatttatt aaaggtcaaa gtagtgaagt attaaatcca tttgtaggag atggtgtaaa
     8581 tgcatcaaat gcacttggac aagtattaat agctactttg tttgcatatg atggttggat
     8641 aaatgttgga gctatatcag gagaaatgaa gtctccagag aaagatttac caagagcaat
     8701 agttggtggt ttatcattag ttatggcagt ttatattata ataaatatag cttatctttg
     8761 ggtagtacca gcaagtgaat tagcaacagt tacttcccca gctacattag tagcaacaag
     8821 attgtttgga aatataggtg gaaaagtaat aactgtgggt atattaatat cagtatttgg
     8881 gacattgaat ggatatttat taacaggttc aaggatacct tatacattag ctgaaatggg
     8941 aactttacca gcatctaaaa ctcttttaaa agtaaattct ggaggttctc ctgtaaattc
     9001 aatactactt ataactgtgt tagcctgtgt atatgcatta tctggtcaat ttaatctttt
     9061 gacagactta acaatatttt caatatgggt attttatgta ttgactttta taggtgttat
     9121 gaggcttaga agacaaaagc cagatttaca cagaccatat aaagtccctt tatatccaat
     9181 aataccaatt atagctatat taggtggact gtttgtaata ataaatcaaa tattgacttc
     9241 aacagtcata tctttaggcg gaatatttat aacactatta ggtttaccag tttattatta
     9301 tatgaaaaaa agataactac ttaatttaag ttagataaat ttgggtaaag gtgaaaatta
     9361 acttttatcc aaatttttta tactgttata caatataagt attatatatt gcttgatgtg
     9421 ctattttgtt tgatttataa tttagtaatt gatgttttaa gataatttaa gaaatgagga
     9481 gtgaaagatg ctaggacttt atgtacatat tccattttgt gtgaaaaaat gtaagtattg
     9541 tgattttaat tcttataaaa tggacataga ttcaaaaaaa agatatatag aagacttaaa
     9601 aatagaaatg gaattatata gtaataaatt atataaagat aataaatata aaaataaaga
     9661 atgctgtagt ctaaataaaa atgacaaaat aacgagtata tttgtagggg gaggtacacc
     9721 tagtatacta acctctgatg aaataagaga agtatttata agtataaaag aaatgtttga
     9781 tatagatgag aatgcagaaa taactataga gtgtaatcct gggacattaa cattagaaaa
     9841 attaaaaact atgaaagaga ttggcataaa tagacttagt atagggctac aagctattca
     9901 agaaaagcat ttgaacttta taggaagaat acatacttat gaagaatttg agaaaaacta
     9961 taaagatgca ttaagtgtag gttttaaaaa tattaatata gatttaatgt acagtcttcc
    10021 aaatcaaact ctatgtgatt ggaaggaaac tcttgaaaaa gttgtacact taaatccaac
    10081 acatatatca gcttattccc ttatactaga agaaggaact gaactataca atatgtatga
    10141 aagtaataaa tttgaactta tagatgaaaa tgtagatata gaaatgtatg agtatacaat
    10201 aaactactta aagtcaaaag gatataatca atatgaaata tccaactact caaaagaagg
    10261 ctataattgt gagcataata ttttatattg ggaatgtgaa cattatatag ggattggagc
    10321 aggagcttct ggctatatta atgagaacag atacaataat gtagaatctt tagaagatta
    10381 tcatttaagt ttggtaaaaa gagaaaaacc aatacaagaa aatgaaattc tttctgagaa
    10441 agatatgata gaagaaaaga tatttatggg gcttagaatg aataaaggca taaaatttga
    10501 agattttaaa aaaaaatttg gaatagattt tagagagaag tataataagc aaatagaaat
    10561 gctattagct agaaagctta taaatcagag ctttgaaggt atacaattaa ctcaaaaagg
    10621 aagagaaata tctaatagtg tatttataga atttatggaa taatttttaa ggtgttgaca
    10681 aaataaattt ataatggtat ataatatata taatcttttt agcactcgaa cttagtgagt
    10741 gctaacagct aggaggtata ataatatgga attaaatgaa agaaaattaa atatcctcaa
    10801 agctatagtt aaagattata tagaaacagc tgaagctata ggttctagaa caatatctaa
    10861 aagacatgat ttaggtgtta gtgctgctac cataagaaat gaaatggctg accttgaaga
    10921 attagggtat ttaattcagc ctcatacttc tgcgggtaga gttccatctg aaaaaggcta
    10981 taagttatat gtaaactctc ttatgagtaa aagcgaattg gatgataatg ataagattct
    11041 tattgaacaa tgtatgaatc acaatataaa tcatataaaa gaattgattc atgaaacttc
    11101 taagctatta tcgcaactaa caaactatac aactgtagca gttactaaga gtttaataaa
    11161 tcaaagtgtt ataaagcaca tacagttggt tgctatgaat gacaataata ttttacttat
    11221 agttgtaaca gataaagggg acttaaaaaa agctaacctt acaactaatg tatatttaga
    11281 tcaatctaag ttaaatttga tttctgataa tcttacaagg aagctcttag gtaaaagtat
    11341 aacagatttg gatgataatc taattgcttt tattaaatac gaaataagtg agtattctgg
    11401 gcttatagat gagcttttaa atgctttgaa ctcaaatatg aaagaagaag atttttctct
    11461 atcattaaat ggagctacta acatatttag ttatccagaa tttaatgatg tattaaaagc
    11521 aaagtcattt ttaaatatgt tggaaaaaaa ggaaacaata gctgacataa ttaaatcaaa
    11581 gggaatacaa aaagacaatc taaatataat aattggtagt gataatgatt gtgaactggc
    11641 acaggattgt agtatagtta cagcaactta taatgtagat agagacttgg ttgggcgaat
    11701 aagctttata ggccctacca gaatggatta tgctagaatt tactctataa taaattatat
    11761 gagtttatta attaatagaa agtaaagaaa ttttaaattg cgaggtgtaa agtcttggat
    11821 aaaaaaaatg agcaacagga agtaagagaa gaaaatgata cttctattaa ccaagaatcg
    11881 gaaactcaag tagagttaga agaggaagtt gttaatgaag aatgtgaaac atcaagtgaa
    11941 aaaactgatg aaaaagaagt tgatgatgaa aatgtaacag atataaattc taagttagca
    12001 gaaaaaaaat tgcaggatga gttggatgaa ttaaatgata aatatcaaag acttcaagcc
    12061 gaatatgcaa actacagaag aagaactcaa caagagaaag agacaatagg cgtatttgca
    12121 aatgaaaaaa taataactga attgatacca gttatagata gtatggaaag agccttagat
    12181 gcatgtgaag ataaggaaga tactatgtat aagggtataa gccttgtaca taaacaatta
    12241 atagacactt tagtaaaatt tggagtagaa gaaatagaag cagaatctaa ggaatttgac
    12301 cctaatctac atctagcagt tatgcaagaa agtgtagatg gagttgaagc aaatcaaata
    12361 gtaatggtac ttcaaaaagg atataagcta ggaactaaag ttgtaagacc ttcaatggtt
    12421 aaagtttctt gctaagtata aattaaaggt taaattattt aacctaatat aaaatattaa
    12481 aataagtaaa acttaattat ataattactc gaggaggatt ttagtcatgg gaaaaataat
    12541 aggaatagat ttaggaacaa caaattcatg tgttgcagtg ttagaaggtg gagaagcaca
    12601 aataatagca aatagtgaag gtatgagaac tactccatca gtagtagcat ttacaaaaga
    12661 tggtgaaaga atagttggag aacctgcaaa aagacaagca gttacaaatg cagataaaac
    12721 aataacttct ataaaaactc atatgggaac tgattataaa gtaaatatag atggaaaatc
    12781 atatactcca caagaaatat cagcaataat attacaaaaa ttaaaatcag atgcagaaag
    12841 ttacttagga caaacagtaa cagaagcagt tataacagta ccagcttact ttacagatgc
    12901 tcaaagacaa gcaactaaag atgctggtag aatagcaggg cttgatgtta agagaataat
    12961 aaatgagcca acagcagcag ctcttgctta tggtatggat aaattagacc aagaaaagaa
    13021 aatattagta tttgacttag gtggaggaac ttttgacgta tctatacttg aaataggaga
    13081 tggaactttt gaagttttag ctacagctgg taacaataga ttaggtggag atgactttga
    13141 ccaaatagta atagattatt tagcagagga atttaaaaaa gctgaaggtg tagatttaag
    13201 aaatgataaa atggctcttc aaagattaaa agaagcagca gaaaaggcta agaaagaatt
    13261 atcatcaaca atgagttcaa acataaactt acctttcata actgctacag cagaaggacc
    13321 aaaacactta aatatagatt tatcaagagc taaatttgaa gaattaacta gaggtttagt
    13381 tgaaaaaact atggaaccaa ctaagacagc tttacaagat gcaggacttt ctacaggtga
    13441 tatagatgat gtattattag taggtggttc tacaagaata ccagcagtac aagaagctgt
    13501 taagaagttt ataggaaaag aacctcataa aggtataaac ccagatgagt gtgttgcagc
    13561 aggtgcttct atacaagctg gagttttagc aggggatgtt aaagacttat tattactaga
    13621 tgttactcca ttatctcttg gtatagaaac tatgggtaat gtaatgacta agataataga
    13681 aagaaatact acaataccaa ctaagaagtc tcaaatattc tcaactgctg ctgataacca
    13741 aacagctgtt gatatacatg ttcttcaagg tgaaagaagt atggcttatg acaatacaac
    13801 tttaggaaga ttccaattaa cagatatacc accagcacaa agaggaatac ctcaaataga
    13861 agttactttt gatatagatg caaatggtat agttaatgtt tcagctaaag atttaggtac
    13921 aggaaaagag caaaaaataa ctataacatc aaacactaat ttatcagaat cagaaataga
    13981 gcaaaagata aaagaagcag aaatgaatgc agaagctgac aaacaaaaga aagaaaaaat
    14041 agaagcattc aatcaagctg aatctactat atatcaaact gaaaaaactt taaatgaact
    14101 tggagataaa ataagttcag gtgaaaaaga ggatatagaa aaagctatag cagacttaaa
    14161 agctgtaaaa gataatcaag atgctacagc agaagaactt aagaaagcta cagatgaagt
    14221 aatgactaag ttccaaaaag tatctcaaga aatgtaccaa aaagcagctc aagaacaaca
    14281 agcggcacaa ggtgcagaac aagcacaaga caatggacct aaagatgaca atgtagtaga
    14341 tgctgacttt aaagaagtag atgaagataa ataagctata taatatgtaa cactaaatga
    14401 taattggaac atatatgatt tgtgaaaatc atatagatgt taaagatata tggacaatat
    14461 acatcataaa gaatataata tatcatagat tatataactt aaagtagaat ataagaatta
    14521 atgatagctt gtagtttatt attctacaag ctatcgtttt gtaaaatgaa ggtggtgtca
    14581 atttttgagt acaaaaaggg attattatga agtcttaggt atcagtaaag gtgcagaagc
    14641 tcaagagata aaaaaagctt atagaaaatt agcaatgaag taccatccag atagaaatcc
    14701 tggagataaa gaggcggaag aaaaatttaa agagataaat gaagcttatg aagttctatc
    14761 tgatgataca aagagaaaaa cttatgacca gtttggacat gatgggctaa atggtcaagg
    14821 cggttttggt ggtcaaggag gatttggcgg tcaaggcttt ggtggttttg aagatatgtt
    14881 cggagatata tttggtgaca tgtttggagg aagcttcgga ggtggaagag caagaagaag
    14941 agggcctcaa cgtggtgcag atataagaca gagtgttact atatcttttg aagaagctgc
    15001 atttggtaaa aagatgtcta taaaagtaaa tagaagtgaa gaatgtgaag aatgtaatgg
    15061 aactggagca aaaccaggaa catcaaagaa aacttgttca acttgtaatg gaactggtca
    15121 agttagaact gttcaaagaa ctccatttgg taatatagca agttcaagac catgtagtgc
    15181 ttgtaatgga actggagaag tgattgaatc tccatgtagt aaatgtcatg gaactggaaa
    15241 tacaagaaaa gttaagacta tagaagtaga tatacctgct ggtatagatg atggtcaaat
    15301 gataaaactt tctggtcaag gtgaagtagg agaaaaaggt gctccaagag gagacttata
    15361 tattgtagtt aatgttaagt cacatccact atttactaga gatggaaatg atatttattt
    15421 tgaaatgcca attacatttg ttcaagcaac tttaggtgat gagatagaag tacctacttt
    15481 ggatggaaaa gttaaatata gtgtgccaga ggggacacaa actggaactg tatttagact
    15541 taaagagaaa ggaatcccta gaattagagg taattcaaga ggagaccaat atgttaaggt
    15601 tgtagttgaa attcctaaaa aattaaacga taagcaaaaa gaactattga gagaatttgc
    15661 gaaggagtgt gggtcgaacg tccacgaaaa gaagaaaacc ttcggacaaa agatagaaga
    15721 tatgtttaag aaaaagtagt taaagtagcc aatatataat catattttta aaatcttagt
    15781 tatttataag attgtcaaag gagatattat gatttttata ttggctattt ttgtataaaa
    15841 aataaatgtg cataaaataa aactgaatat aaataatttt ctgaaaaata cttgtcctaa
    15901 aatatataat atgctataat ataatcaaat aaaagaaagg ggtgattcca gtgggaacgg
    15961 atgtggcact gccaaatgat gaagaaaact ttattgtaat atacaaaata aaattagata
    16021 cttttagtgc tagattaagt actggaaaaa ctcatgagct gggataatta ttgtaattac
    16081 ctagactcaa acttttagta gtaaaatagt aatattatat agacaaggta tctaattttt
    16141 aaatcttttg tatgaattaa aatttttata aagggtatat ctgattagaa tgtaattagg
    16201 gaaacctagt tgtgaattac ttttagtata tacatgtgta aagaaatatg tatatggtat
    16261 tgggtattgt gctagatgtg tatgtaaagg aatatgtgtc tagttgggta tatgtaaaga
    16321 aatatactta taatctatga aaaattttta gtataaattg ctaaaacatt gagattgttt
    16381 tttatagaaa gttagtttat accaactaaa aatgtgctta agatttgttt gatgtttaat
    16441 tataaataag gttgtagata aaaaaataag taaagacaaa ggtttaaaat acttgcttta
    16501 aatatagttt gttttgaaca cttgccaaat taaaataatt aataaaatag acatcttctc
    16561 atagtaaaat atactgtgag aggatgtttt tgctttgttc ataaactttt atgaaaaatt
    16621 tgcatagtgt cacattttaa tatactatat atataataaa atatagataa aattaaatga
    16681 ttcaaaagag aaaggtgaaa agagatgtat tatataggtg tagacatagg aggaacaggt
    16741 atacaagcag gagtagttga taactatggg aagataattt ttaggtcaga gtgtaaaaca
    16801 gtaattgaaa aaggatttga aggaatactt aatgacataa aaataatgat ttataagttg
    16861 ttggaagata ataaacttac tatgagtgac ataaaatcta ttggatttgg tgttccaggt
    16921 tttataaata aagaaggatt agtaacttgt gtaaatttaa aatggaataa aaaagctttt
    16981 aataaagagt taaaaagaag atttccagat gttgaaattc atggagaaaa tgatgcaaca
    17041 gtagcagcac ttggagaagc aaagtttgga agtatgaaag gtgctaatgt tggagtatta
    17101 tatactttag gaactggtgt aggtggagga atagtaatta atcaaaaagt cttttctgga
    17161 gcacatggat tgggttcaga aataggtcat caaattatag gagaaaacta ttttaattgt
    17221 aattgtggaa ataatggttg tgtagaaact ttttgttcag caactgcaat tataaagtat
    17281 tctcaaaaac tgatagaaga aggagaaaag agtaggattt tagatttggc agagggaaat
    17341 ttagaaaatg taaatgcaaa gatggtgttt gatgcatata gagaaaatga tttagtggca
    17401 attaaagtaa taaatagatt taaagagtat ttagcaaaaa cttttgcaaa cacaataaat
    17461 tctttagacc cagaaattat atctatagga ggaggcattt ctaaatctag cgatataata
    17521 cttgatggaa tagaaggttt ggtaagaaaa tttgtattat acaaaacaga agatatagct
    17581 acaattacat gtgcaacttt aggttcagac gcaggaatta taggagcagc ttttttataa
    17641 tgtaaatgaa ctgcttattg gaggcgtgat gatatatggg taaaacaaat gctagaagaa
    17701 cgcttttttt gatagcaata ggttcaggaa ctatgctgaa tcctcttaat tcttctatga
    17761 tttcacttgc tttacatagt atacaaaatg attttcatat tagttttaca actgtatctt
    17821 ggattgtttc agcattttac ctagctagtg ctattgcaca accagtttca ggtaagatag
    17881 gagatttaat aggtagaaag gtcttatttt tgagtggttt gatgcttgta attttatcag
    17941 catccatagt accacttgtt caatcattct ttatattgat ttttataaga gtaattcaag
    18001 caattggaac aagtacccta tatccatctg gagttgctct tatacaaaat aatataaaag
    18061 aaagacaatc ttctgcatta gcagttctaa ccatttttgc ttcaactaca gcaggattag
    18121 gtccgacact tggaggatta ttgttggact taggaggatg gcatgctata tttcttgtaa
    18181 atatacctgt tgttttggtt tctatatgct tagggtattt tttattccca aaagaagtaa
    18241 aagaaaagaa atctataaaa gaaactttaa aaaatgtttt gtcaagatta gatattattg
    18301 gcatacttct attttcaatt gcaatgattt ttatattact atttttgcta tctatgaagg
    18361 aatcttttaa cttagaacaa cttgtatttg gtataatact gatgtgtctt tttatttggc
    18421 atgaattgag aacaaaattg ccatttatag atattaagct ttttgtaagt aatcctaaat
    18481 tgtcaaaagt ttacttgcaa tttattatat taaatttctt taattatatt ttgttttttg
    18541 gtcttcctag ttattttcaa gatgccttac attatagtgc aaagtcgact ggattattta
    18601 tgctatttat gtctggtata ggtatattta tatctccatt aactggaaaa tggatagata
    18661 aatctggaac tagatttcct cttgttacat catcaatctt tatgtttatt agtgcagtac
    18721 ttctttcagt agtttttatc catatacctg taataggaaa gggtattata ttatcattag
    18781 caggtattag ttatggagtt ggtaatgtag ccctgcaatc ctctatgttt gaagaaagtc
    18841 ctcgtgacag tatagggaca gcttctggtc tgtttcaaac tagtcgttat ttaggttcta
    18901 ttttagcaac tgttttactt ggaatggtat ttgaagtaaa tataacatca gaacaatttc
    18961 agatattagg ttatgttatg gtggtcttag gtacaataag ttttttatta aattttatat
    19021 taaagaaaga attaagaaaa gtagaatagt ggatttatgg atttaataag tctaataact
    19081 gataagttta aaaatattgt ttgtaaaaca taactggttt gatatttatt aattctaagt
    19141 tttgaagcat agagtactcg ataagtatac aatattttga attaaaatta agcagaaatt
    19201 tgaactagtc tttcaaatct ctgcttaatt tagtagttaa atttttttat agagaaaata
    19261 agatttgaca cttagataaa aaatcattgc attttcaaaa ttctttaaat caaacccaag
    19321 tatctcatta atcttttcca atctatatac tagcgtgttt ctatgtaaaa acatactagt
    19381 agatgtctta gaaatattta aatcattatt tataaatttt attcctgtat atagaagttc
    19441 ttcgtcaata gatttaatct tattaaaatc tattaattta ttgagtatat tatcctcatt
    19501 aattaaatct aaagttttat atctaataag gtcaaacaaa tttaaaaact tattttcagc
    19561 gttgtctttt ataatatgag gtagtaaatc agaacataac tgtaatctaa aatcaaaatc
    19621 agctttacta tctaattcac caagataaaa aatataagta ctttgataag tttcagtttc
    19681 aattatattt tgtgtagtta cattaatatc taagttatat ttagaattta ctataaaaaa
    19741 accattgtgt tgtattatat ttatatcttc aaataaatta taaaaaatat tttttagata
    19801 ttcaaagtct atatgagatt ggtaaattaa ataaaagttg ccttttaaat tatcgagatt
    19861 atttttatct agaaaatcaa taacataaat agcattattg aaagtaacta aatggctact
    19921 agcatctaac ttttggtttt catatggata gatatttatt tttttattaa gctgtctttc
    19981 taaaaaatct tttaactctt ccataaagtc tccttaaaaa taagattata ttcttatctt
    20041 atagctaaag tagtttcttt gtcaaatatg tgtactctac tattgtcaac tgaaagtgtt
    20101 aagtcatcac caacattaat tctgttttta ctgtcaaatc taattgttat cgtattttcg
    20161 cctagtttta agtgagcata agtttctgct cccataagtt cttttatttc aattcttgat
    20221 ttaaaacaag tatctggatt agatttaata aaagtatctt ctacatgaat atcttctggt
    20281 ctgataccta taacaacttc attattaata taattgtctt ttattaaatc atagtgtttt
    20341 tctttattca atttaagctt aatatgttca ttttgtgcat aaatttcatc attttcttct
    20401 attaatatta catcaataaa attcatctgt ggtgctccaa taaatcctgc aacaaatata
    20461 ttagctggat aatcgtatac atcttgtgga gttgcaatct gctgaactac tccatccttc
    20521 attactacga ttctatcagc catagtcata gcttcaactt ggtcgtgtgt tacatagata
    20581 aatgtagttc caagctgttg gtgtagctta gttatctctg ttctcatagc agttctaagt
    20641 tttgcatcta agttggatag aggttcatcc attaagaata ccttaggatt tctaactatt
    20701 gcacgtccta aggcaaccct ttgtctttgt cctccagata atgttttagg ttttttattt
    20761 aaaagaggta gtaagtctaa tattttagca gcttctttaa cttttttgtc tatttcatct
    20821 ttaggcaatt ttctaagttt aagtgcaaat gccatgtttt cgtaaacaga taagtgggga
    20881 tataaagcat agttttgaaa aaccatagct atatctctat cttttggttc tatatcattg
    20941 acaagtttat ctccaatgta tagttctcct tcagagattt cttcaagacc agcaatcatt
    21001 ctaagtgtag tcgatttacc acaaccagat ggtcctacta aaacgataaa ttctttatct
    21061 ttaatgtcta tatttatatt ttttactgca ttaaatccat ttgagtaaag ttttgatata
    21121 ttttttaaaa ttacttctga cataatatcc tccttgatat ttaaatttta attatatagt
    21181 atcaaatttt tttattatat taaatgtgta aaatgactaa atgtatttgt ttaatatgta
    21241 tagttagttt ttatattaag atttattata atttagaaaa tat
//
LOCUS       DAMKMI010000059        21279 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMKMI010000059 DAMKMI010000000
VERSION     DAMKMI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.060"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            171..1181
                     /locus_tag="PC429_002006"
     CDS             171..1181
                     /locus_tag="PC429_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF3847511.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1317..1805
                     /locus_tag="PC429_002007"
     CDS             1317..1805
                     /locus_tag="PC429_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3847512.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1981..3909
                     /locus_tag="PC429_002008"
     CDS             1981..3909
                     /locus_tag="PC429_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3847513.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFENLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4086..4496
                     /locus_tag="PC429_002009"
     CDS             4086..4496
                     /locus_tag="PC429_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF3847514.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4518..4820
                     /locus_tag="PC429_002010"
     CDS             4518..4820
                     /locus_tag="PC429_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF3847515.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5037..5729)
                     /locus_tag="PC429_002011"
     CDS             complement(5037..5729)
                     /locus_tag="PC429_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF3847516.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5755..6942)
                     /locus_tag="PC429_002012"
     CDS             complement(5755..6942)
                     /locus_tag="PC429_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3847517.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPKKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7278..9155
                     /locus_tag="PC429_002013"
     CDS             7278..9155
                     /locus_tag="PC429_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3847518.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNIGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYIEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVISCKSSYVSTNDNIISLVLAG
                     N"
     gene            9510..10916
                     /locus_tag="PC429_002014"
     CDS             9510..10916
                     /locus_tag="PC429_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF3847519.1"
                     /translation="MNTGFPKEFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10944..11792
                     /locus_tag="PC429_002015"
     CDS             10944..11792
                     /locus_tag="PC429_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3847520.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12453..13145
                     /locus_tag="PC429_002016"
     CDS             12453..13145
                     /locus_tag="PC429_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847521.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            13142..14077
                     /locus_tag="PC429_002017"
     CDS             13142..14077
                     /locus_tag="PC429_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3847522.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPYDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDRDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            14225..15148
                     /locus_tag="PC429_002018"
     CDS             14225..15148
                     /locus_tag="PC429_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847523.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            15132..15902
                     /locus_tag="PC429_002019"
     CDS             15132..15902
                     /locus_tag="PC429_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847524.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15912..16694
                     /locus_tag="PC429_002020"
     CDS             15912..16694
                     /locus_tag="PC429_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847525.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSINLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            17071..17607
                     /locus_tag="PC429_002021"
     CDS             17071..17607
                     /locus_tag="PC429_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF3847526.1"
                     /translation="MNLKGTKTEKNLIAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17639..18028
                     /locus_tag="PC429_002022"
     CDS             17639..18028
                     /locus_tag="PC429_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF3847527.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            18065..18451
                     /locus_tag="PC429_002023"
     CDS             18065..18451
                     /locus_tag="PC429_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF3847528.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPITKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18578..20020
                     /locus_tag="PC429_002024"
     CDS             18578..20020
                     /locus_tag="PC429_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF3847529.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEVSNYTNIQHV"
     gene            20317..21132
                     /locus_tag="PC429_002025"
     CDS             20317..21132
                     /locus_tag="PC429_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3847530.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7869 a         2479 c         3895 g         7036 t
ORIGIN      
        1 acatattttg ggtattgtat actggaatta gagtaattaa tttatttttt aacatacaaa
       61 tgattttatg aaaaaaaaca agactcaaaa atttttatat tctttgatag aataaaattg
      121 agagtaaatt tattgaattt tataaaattt ataaggggtg agtctaagat atgcatgaag
      181 ataaaaatga tatatttgaa tcaatagata ctgaggattt tgataataaa acagaagaag
      241 aatgtttaga gctagtaaga aaaaatggat tgaatttaat atacataaaa aatcaaacag
      301 aagagatatg tctagaggct gttagacaaa attgtaatgc aataaaatgt gttcagaatc
      361 agacagaaaa aatatgtata gaagcagtta aacaagattg gagaatgtta gaatttgtgg
      421 aagagccaac agaagaaatt tgcatgttgg ctattaacca agatggaaca gcattaaaat
      481 atatagaaaa tcagacagag gagttatgct taagagccgt tgaaaaaaat ggtgcggctt
      541 tggagtatgt aaaagaacaa acagaagaaa tatgtataga agcagttaga aacagcgaat
      601 ttggattggc aagagtgaaa aatcaaacag aaaaaatatg tatggaagca gttaagcatt
      661 gtagctataa tctaaaatgg gtagaaaatc aaacagaaga aatatgtatg gaagcagtga
      721 gacaaaatgg gcttgatttg aaatttgtaa aaaatcaaac agaaacaatc tgtttaagag
      781 cagtgagaca aaatggaatg gcattagaat ttgtaaagga acagactgtt ggtatatgct
      841 taaaggcagt aagacaatat ggaatggcat taaaatttgt aaaagaacaa acagaagaaa
      901 tttgtacaga agctataaaa caagataaaa gagcacttag ttttgtaaaa ggagataagg
      961 aaaaatacaa ggctctatat gataataatg aaccatttgc aaagagatat gttagaaatg
     1021 taatagaaaa agaaaacaga gcattgatta aaaaaggaga agaaaatgca aaaatcaaaa
     1081 ttgcaggaaa actttatgat agaggcatgt cttttgaaga tatatcagat atagttgaga
     1141 ttgaaataag caagttaaaa gtgagtttag gtgtttttta aagtaataaa tgtaggtgtt
     1201 ttttatggga aatagttaat aaaaaaataa tcgattagct ctttgtagga cagcaaaatt
     1261 agtgatataa tttgatgtat aataaattat ttttattaga taagggggaa ttaaagttga
     1321 agattacagt attataccaa acaagaagtg gaaacacaga aagggtcgct aagctgatag
     1381 aagagggcgc taaaaaggtg gatggagtag atgtaaagct tatgagactt gataatattg
     1441 atttagatta tttaaatgaa agtaaagcag taatatttgg aacacctact tatttagcaa
     1501 atacaacttg ggaagtaaaa aaatggtttg atgaagattc aaaaaaagtt aatttagctg
     1561 gaaaattagg agctgttttt gctacatgtg attatatatg tggaggtcca gatgttgcga
     1621 tacttactat agtaggacat ttgatggtta aaggtatgct tgtgtattct ggaggtggtt
     1681 ctttaggaaa gccatttata catttaggac atgtacattc aattgaaggt ccagagttac
     1741 aagatgagaa ggctataata tttggtgaaa gaattgctac taaggcaaaa gaactatttg
     1801 cttaatttct atatgcataa taatgattag agtaaattta gggttttaag ggagatttga
     1861 agtaaaagtt ataaaataaa aaattttcca taaaattact caagatgaat tataatataa
     1921 ttataaataa attatattat taaaatattt aaaatcaaac gtcaatagcg agggaagttt
     1981 atggtatggc atatagaata tgagattttt tctgcaatta tagttatttt tttaatggta
     2041 tactttttta gaagtaaatt tatacctact ttacaaaaca aaatatattg tgcactttta
     2101 atattctcat ttttatttat aatttcaaat attttaggtt cattttgtct aaataatata
     2161 gacaaaattc caatttttat tactttttta ttgaatcaga tatacttatt gttattgcca
     2221 ataccagcag cacttgtatc attttatgtt atggctatta tttatcaaga cataaggtat
     2281 atgaaaaaaa gattattatt tttatctata ccacttataa tttgtataat cttatctatt
     2341 acaaatcctt ttacgcatat tctatttgga ttgtcaaaag aaacaggtta tatacgaggt
     2401 tgtggatatg ttgcaacttt tgcatctttt ttcttttatg caatatattc agcattttta
     2461 tcttttaaat atagaaaagc aatgcatgaa tcaaaaatat gggctattag aggttttctt
     2521 atagtttcaa ttgtggcaat tatcttacag gcaatttttt tacagtatct tcttacagga
     2581 actgcatgtg catgtagttt acttttgaca tatctttcta tgcagaatcg aggtcttatt
     2641 attgatgacc ttactggtgt actcaataga caatctttta ttcaagagct agatttgaat
     2701 atcaatactg aacaatcagg atttattatt acaattgcgc ttgatgattt taagtttatg
     2761 aatgaaactt ttggaacaaa aaatggagat attgcactaa aagaagttgg aaaatatttg
     2821 attcagatac atgatgtaaa ccatgtatat agaatagggg gagatatatt ttctattgtt
     2881 gtcaatagtc aattaggtat agaaccagat gatattatca gaaagataga agaaagattt
     2941 aaaaagcctt ggctagtaga agagataagt tttaatttag caactagtat tgctgttgtt
     3001 tattacccag aaaatgcaga aacgacagaa gatgtaattg ttgctataga tttctctata
     3061 catgaagcaa aacgttctga gtcaaggcga gttgtttatg cagatgcgtc tataagtgaa
     3121 aagataaaga agaaacatgt tattaaaaat tgtttaaaag aagcattaaa aaatgatggg
     3181 tttgaagtgt actaccaacc tatattttct aatagcaggg gcaggtttac ttcagcagag
     3241 gcattactta ggctagaaca caaagagtta ggttttatac ctcctgatga atttattcca
     3301 attgcagaac aaacaggatt gattaactca attggattag tagtctttga gaaggtatgt
     3361 agatttatag ctagtgatga gtttgaaaat ttaaaattgg ataatatagc tgtgaatctt
     3421 tctgtagtac aatgtatgca aaaaaactta gctgatgatt tactttatct tatgaataaa
     3481 tataatgtat caccaactaa atttaaactt gaaattactg agactgtggc tatgggctct
     3541 tttaatataa taaaagaaac aatggaaaga ttaattgatt taggtgttaa gtttgcactt
     3601 gatgattttg gaattgctta ttctggagtt actaatatgc tttctttacc attttcagta
     3661 attaagttag ataagagttt gatttggtct atgaatgaag atagtaggca taaacttaca
     3721 gttgagacga ttatagcact tataaataaa ttgaatatga aggctgtagc agaaggtgtt
     3781 gaaaccatta agtgtgctga agatttaata gctatggagt gtgaatattt gcaagggtat
     3841 tatttttcta aaccagttcc agaagatgtt tttaaaaatt tgttgaaaga aaatactttt
     3901 acaagttaac accctttatt tttttaatta caactttgtt cattgacact gccagcgata
     3961 tattataaaa ttaacacaat aataaatatt aaatttatta tacatattat taatattaat
     4021 taatggagta aagatttatt atatatttta tttaattatc ctaaagtgag aaaggaagat
     4081 gtgacatgaa taaaaagaaa atattagtgc caattgatgg gacagaaaga agtatgcatt
     4141 ctttggaatt tataaaagga atatttaaaa aagatgaagt tgaagtagaa ataatgaatg
     4201 taaaagaact agtatttatt gatggcatat ccctggctga agagataaaa aattctgaaa
     4261 atttaggaag aagaatcttg gataaagcag cagagataat gggagagtat gatgttaaag
     4321 tacattttac ttttggatat ccaggagatg agataattag aaaagcaaaa gaagataatg
     4381 tagattttat agttatgaca aaatctacta aaaaaggtct tacaagaatg ataggttcag
     4441 taacagcaag tgtagttaaa caagcaaaat gtatagtgat gattgtaccg gagtagaatt
     4501 agaaaggtat ttgaaatatg aataaaaaga tagtagcagt ttgtgaaagt gaaagaagtt
     4561 taaactctct taagacagct gcaaatgatt taggatttga tttagtttat gaaattcaag
     4621 aaaatgataa tataaaaaat gaactttcta tttatgatat agaagatgca tccatagtgt
     4681 tatttgttac aagtgattct atagaaagca tagagaagat tgatagattt atagatagag
     4741 aattttatga agttgatcca aagtatataa tagaggatgc aaaatctata gtaagtgaaa
     4801 ttttaataga cttaaattaa tcgtgggtgt cgcactaaga atagagcgac gcctttttat
     4861 atattttaca caagaaataa agaggttgta tcaaaatata gataagactc tttttcattg
     4921 ttgaaatgat gaaaatatat ttgatttcat acaaaaaaga gtacctcata actaaaaagt
     4981 tcactttgag acactccatt tttatatgtt agttgcattt aaggtaaaat ataagtttaa
     5041 tcatgttgaa ataactgaat tacatcttct aaatgattgt acattgatgt ataacctaag
     5101 taagagtctt tactatcaaa tgcaatcatt atcttgttat ggtggctaat catatccttg
     5161 agagaatttc tatatttaaa accgaagtat tggttagaag aaactgtttt aggtattaac
     5221 tcataatatt ttataaaaat tggattatga gaagctaaaa ttatctcttt gtgaaattgc
     5281 aagtcagcat cataacattc ttctatcaaa ccatgagaaa ccttatccaa ttcctctatt
     5341 gactttaggt aagtatattt tattttttga agttctttag atgtagcatt taaaattgct
     5401 agtttagtag cttcactttc gataagttgt cttgctctta aagattctac ataaaattct
     5461 tgtatgttat gatttttata gatttcacca tcatcaaata ggtcaacttt tttaaatcct
     5521 tcaactatag gacttgaata aggcacagct ctaagtattc cttccttaca gagtatattt
     5581 atggcatttc ttacaggtat tctacttatg ttaaataggt cagcaatctt tctctcactt
     5641 ggtaatttac tattaatttt aaaagttcca tttagaatgt ttaatttaat ataatcaata
     5701 attttttcag tggcatttaa gcttttcata aaaactccta aacatataat ttttttatga
     5761 ttgtattata tcatagttaa gagttaaatc tactttttta taatatgtat aagacaggat
     5821 agaggccaaa atagctataa tagccaagat aaagtatcca tattgtattg aagtatattc
     5881 cgatatttta ccaactatca ttggaaatac agacattcct atagcataaa ttgcttgaaa
     5941 aaaacccatt gcagtagatt tctgatgttt tggtacattt ttcatagact cgctagtaag
     6001 gtaagaaagg agtatacctg tagatatacc tggtagtact tgtagtacaa atattatatt
     6061 aatatttttt attgttggaa ctaaaaagca ataggctgaa actaaaataa aaactaaagg
     6121 aatccaaaat tttggtccat acttagtaca aaatttagta gaagcaaagc cagaagaagc
     6181 aacagcacac accatgtaaa atatagaaga tagacctatg agggtagaac tagaacctaa
     6241 gtttttaaga atttgagtag taaatgacat tgttgtagac atttgaatac cctgttgaat
     6301 taatgccata aaggaaaaaa atacaagtct tttatccttg cagatagata aatatttttt
     6361 aattgatatt tttttaggta ttttttttga attatgattt tttatattta aggctagaat
     6421 cgttccaata actgaagaca atacactcat gatacataag gctgtcattc ctaatttttc
     6481 ataaaataaa gtactcataa caaatccaat caacattcct agattgttta ccaatattat
     6541 cgtacttgta gctttttgtt gatgttctgt agaaaaaaag ttggtatagt gaaccatgta
     6601 agaaatccaa gttgatgatg atattccaga taaaatgtta gctataagaa aaccattgcc
     6661 tccaggagaa aagactctta taagagatgc taaaccagca aagaaaattc cagacagtat
     6721 aaatatttta tgtttattta tagaatcagc aaatactcca acaggaagcc ttaataaaca
     6781 ttgtgagatt ccatatgcac ctattattat accaatcata tttgtagata cattacatgc
     6841 acttaaatat ggattttgga aaggtatata tacatattgt gaaaaccaga aaaatgtaac
     6901 aatagaaagt aaaagtaatt tttgattaga tatttttttc atattatcct ccgtatggta
     6961 tttttataat gcaaataata taccatttgg attttttaat caagtacttt ctctattaaa
     7021 aaaaatttaa atatataatt attatttaca aaaaaattat actttatagt tgaattaaga
     7081 aaacgcttat gatactataa agttaacaaa tttgaatata taacagtaga cgtgttactg
     7141 gtaatgcagg caagatcgaa tcaattttta tagattatct atagagtact atgggtataa
     7201 tctatttatt tggttcgatt tttttgttta attggccata aaacaaaaag cctatagttg
     7261 aaaatggagg attttttatg gattataaaa atatagctca agaaattcta ctaaatgtag
     7321 gaggaaaaga gaatgtaaat gaagtgactc attgtatgac aagacttaga tttaaagtca
     7381 aaagcgcttc taaagtgaat aaggataaat tatctaaaac tgaaggagta ataactgttg
     7441 ttgagagtat gggtcaaata caagttgtaa ttggaaataa agtaaaaaaa gtatatgatg
     7501 aggtcataaa aattgtacct caatcaaata acataggtaa aaagaatgaa agtcaagaaa
     7561 agcaaggtat aattaattct atattatcag cagtagctgg tatatttaca cctacaatac
     7621 ctgctatagc aggggttggt atgattaaag gtattttatc agttcttgct atgtattata
     7681 tgaataaaaa tggagtagat ataaaagaaa cgcaaagcta tattatctta aatgctatgg
     7741 ctgattctat attctatttt atgcctatta tactaggata tactgcggct aaagtattta
     7801 atgcaaataa aataatatct atggtattag gagctacact ttgttatcca acttttactg
     7861 ctttgatggc aggtgaagaa tctgttagat ttttaggatt ggcagtaaca aaagctacgt
     7921 atacatcatc tgttattcca ataattattg ctatatgggc tctttcatac attgaaaaag
     7981 tattagaaaa gtatatacct gaggtaatta aaataataat ggttccaact ttatcattgg
     8041 ttgtaatgtt acctgcaaca ttatttttat ttggaccaat cggaatttat ataggaaatg
     8101 tcataaactt ttcttataag tatatttatg aattaagccc agcattgtgt ggagcatttg
     8161 taggtgggtt atggtgtgtg ttggttatat ttggtgctca tagagcatta ttaccaatag
     8221 gtataagtga tgttgctcaa acaggaagac aaaatttatt ggcatttgca ggagctgcaa
     8281 acttttcaca agcaggggct gctctaggag tattctttaa aactaaaaat caaggactaa
     8341 aaacaatttc tatgtctgca actataacag ctttatttgg tataactgaa cctgcaatat
     8401 acggtgccaa tcttagatta aaaaaaccta tggtatgtgc cgtaatatgt ggtgcatttg
     8461 gaggtgcgat aatgggaatg ggtggagctt atggtaatgc atttgcaaac caaggtgttt
     8521 taactatacc tgtatatgct gaagcaggtg cattaggttt cttaagttat ctaggtggtt
     8581 gtgctatagc attttttggt tcagctattt caacttatct agttggattt gaagatttgg
     8641 aagagtcttc taataaggaa aattcatcta taaaagtaga aacgaaggat ggtgttattg
     8701 atataacttc acctgtagag ggcgaatgta tagaattgtc agaagtaaag gatgatgtat
     8761 ttgcttcaaa agctatggga gaaggtatag ctgtgttacc tacaaaagga gtaataacag
     8821 cccctactga ttgtgaagta gcttctttat ttccaacatt acatgcaata ggattaaaat
     8881 tagataatgg agcagaaatg ttaatacatg ttggaatcaa tacagtagaa ttaaatggta
     8941 aatactttac aaaacatgtt aatcaaggag atttagtgaa aaaaggtgat aaacttatat
     9001 cgtttgatat agataaaata aaaaaggctg gttatgatgt aactacgcca gttatagtaa
     9061 acaatacatt tgattttgga caagtaattt cttgtaaatc tagttatgtt tcaaccaatg
     9121 ataatataat ttcattagta ttagctggta attaagttaa tttgactaga gataagtaaa
     9181 ttgatttaaa aggaaataag taaatcaaca attaaataca tctaaaagta agtttattgc
     9241 aatgaattgt taaagaaaat aattaatatg gagtaaatct attgcaatga agtaacaaag
     9301 aaaataatta atatagagta agtttattgt aatgaattgt taaagaaaag agttaatata
     9361 gagtaagttt attgtaatga attgttaaag aaaataatta atatagggta aatctatctc
     9421 aatgaaataa caaagaaaag agttaatata gagtgaactt attgcaatga atcaacaaag
     9481 aaaataatta ataaaaagga gaaatattta tgaatacagg atttccaaaa gaatttttat
     9541 ggggagcatc atcttctgca tttcaagtgg aaggagcttg ggataaagac aataaaggaa
     9601 aaaccgtagc cgactataat tcttttaaaa agtctcattt acaggcagat actaaagtcg
     9661 catcagactt ttaccataat tatgaagaag atattgaatt aatgaaagaa ttagggatga
     9721 aaacatatcg tttttcaatt tcatgggcaa gaataatacc agatggtgaa ggagagataa
     9781 accagaaagg tttagacttt tataataaga taatagacaa actaatagag tgtgatattg
     9841 agccattcgt aactttatac cattttgacc ttccatttaa attagtagag aaatataatg
     9901 gttgggaaag tagagaaact gtctatgctt ttgaaagatt tgctaaaata tgctttaagc
     9961 attttggaga tagggtaaag tattggcaac ctcataatga acagaattta attgttagag
    10021 tggaggagag aattaatata tatgatgaaa cagattcatg gaagatagac aaaatgcgtg
    10081 ctcagatgga ttataatctt tgcttagcac atgcattagc agtcaatgca tgtcatgaga
    10141 tgataaaaga aagtaaaatt ggagcagcag tatcatcatc tgtaacatat cctcttactt
    10201 ctaagccaga agatgtatat gcagctagaa tgaatgataa ttttaaagtg tactatatgc
    10261 ttgatatgca tcattatgga gaatatccag ggtattatat gaagtatctt gaaaaaagaa
    10321 atatagttcc tcacatggaa gatggggata aagagatact taaaaaagct aaaatggatt
    10381 ttatagctgt aaattattat aggacaaatt gtgcagaagc tttacctgaa gattcacaac
    10441 atccatttgg attaagagaa ggaactgttg attttagtat gtatggacta tttaaaatgt
    10501 caatgaatcc taacttagag gcttctgagt atggagcagc aatagaccca tcaggtctta
    10561 gagttgcctt aaatgaatat tggcaaagat atcacttacc tgttataata acagaaaatg
    10621 gtctaggagc taaagatata ttagaagatg gaaaaattca tgatgactat cgtatagact
    10681 atttaagaag tcatataaat gcatgtaaac tagctattga agatggtgta gagatgattg
    10741 gatattgtcc atggtcattt acagaccttt taagttcttc tcaaggtttt aataagcgtt
    10801 atggacttgt ttatataaat agaactgacc atgaaatttt agatttaaaa agaatcaaga
    10861 aagatagttt ttactggtat aaagaagtta tagagaacaa tggaatagta aaataaacat
    10921 aaatatatat tgagggtttt agtatgtaca ttacaaaggt attaaataat tcgttgctac
    10981 ttgcaaagga tgataatgat gaagaaataa tattgatggg gaaaggcatt ggccataatt
    11041 ataaggccgg gtctgagtta aataaaaaag atatagaaaa gatttttgtt cttcatgatg
    11101 aaaatataaa aaagagtatt atacaattgg caacagaaat agatgcagaa tattttggta
    11161 tagctcaaat gataattgca tatggaatag aaaaatataa cttaaaactt atgaaccata
    11221 tatatttggc cttaactgac cacattgcct ttgcagtgag acgttttaaa gaaggtataa
    11281 tgattgaaaa tcactatttg ttcgaaatca aggaatttaa tccaaaagag tatgatatag
    11341 gaaaatatgc tattactgtt tttaaggagg ttcttggttt agacctacca gaagaagaga
    11401 ttggtaatat agcaggccat tttataaatg cacaacaaga taatccttat agtgatagaa
    11461 ataagagaag tgctaaaatt gtaaatgcta ttttgcaaat agttcattat cacttttcta
    11521 ttgtatataa taaagagtca ttttattata ggagatttgt aatgcatcta aaggcctttt
    11581 cacaaagatt tctggctaat gaaccatcaa gagaaaaaat agattttata tatgagcaag
    11641 ttcaaaaaaa ttgtaaggaa gagtatgaat gtgtgaaaaa ggtaggagct tatatgtata
    11701 aggaatactc cagagaatta ccaagacaag aggagttata tttgatgatt catattcata
    11761 agattttagg agaattatgc gaaaatagtt agaatatctt aatatgctag taatattata
    11821 aaaaattaaa aattaactac acttcaaata tgaataattg cattacttaa gggggttgtc
    11881 ttaaaattga tttttttaat taaatttgaa gctcaactcc ttttttaaac ctttatatca
    11941 ttacaacttt aatgtttata cgcctgtact tcagatgtat atctactttt tacatttctc
    12001 aacatgcaaa tctaccattt tttgcagtat gttaattgaa ataaaccttt gagttaaccc
    12061 taaatttatt ctggacttat aggaatagtt ccaaagatac tttaaaatat tttctggaat
    12121 gattgcttat ttcacatatg ttttattgtg atgttttgat ttaataaatt cttcaactct
    12181 gtatatagta aatgtattat catgtttgtt ttattcaagc acacttataa tacgcttgat
    12241 tttttcacaa tgatgaaata tactaatggg tgttataagt ataagaatag cattacactt
    12301 gaagaagcaa gtgataaatg aggtataaat cctcatatga taaactacta tttcattgag
    12361 gagcatattc taagtgatgt caagatacta caatatgact aaattcctag tgataaaaaa
    12421 gtctacgaat gagtgtagaa aggggaacta agatgaataa agttttaatt atagatgatg
    12481 ataaagatct ttgtattttg ttaaaaaaga gtatctcaat tgaacagatt gaagcaaact
    12541 attgccattc tgggagagat ggactggttg aactttcaaa acaagattat cagttagtag
    12601 ttctagacgt gatgatgcca ggtatggatg gttttgaaac tatggaacaa atacgaaaat
    12661 atagcagtat tcccatctta atgctgacat caaaaagcga taattacagc aaagtgcatg
    12721 gattgcgagc tggtgcagat gactatctaa ccaaaccatt tgaaattaca gaatttactg
    12781 ctcgtgtact gtctttaatc cgacgttaca cccgttttaa tccaaaaaat gaggaaccac
    12841 agcaactttc tttcaaaggt ttgttgattg accttgacca gcatacagtg actaccaatg
    12901 gcgaacctat gttgcttcca cccaaggaat ttggtgtact tctttactgt gcaaggcatc
    12961 agggtaagat tttgacaaaa cagcagattt atgaagccgt gtggggtgag ccatatgtat
    13021 atgatgacag caatatcatg gctattatca gtcgcctaag gaaaaaaatt gaactagacc
    13081 catcatctcc catctatttg caaacaataa aaggaattgg gtatcaattc aacacggagg
    13141 tatgaaaatg gatgaacaaa cgataattct tttcttactc ttgctgtcga ttggtctact
    13201 ttttctttcc atatttgctt tttccaagct tgggcatatt tacaagcgcc taaaagatat
    13261 agaggagata ctggctgatg tacaaattgg agacgaaaat cgtaaaatac taattaagcc
    13321 atatgatgtt atggctccat tggtttatca gcttaatgag attgtctacg attatgaaaa
    13381 taagttactt tctctgaaaa agtcggataa agctagcaaa caacttatga caagtttatc
    13441 tcatgatgtt cgtacaccat taacaactct gattggttat ctggatgctg tacacagtgg
    13501 tatagttatc gaacaagagc gggaggaata ccttgaaatc gctaggcgta gagcctacga
    13561 cctcaaggat tacattgatg ttctttttga ctggtttcgg ttgaactctg atgaatttac
    13621 gctgtcaata gaatctgtgg aaattgcaga gttgtcacgc aatattttga aagactggat
    13681 tcctatattc catgaaaaga aactgaattt tgaaattgat attccagaaa atcgactaat
    13741 ggtaaacctc gaccgagatg gatattcccg tgtggtcaat aatctggttc aaaatgttct
    13801 tgctcacagt aaggctcggc aaatcaaaat taccatgagt gaggattcca gaatggtatt
    13861 actccgtgtt gaggatgatg gtgttgggat tgccagagaa aatttaccac atatttttga
    13921 gcgactgtat aagtgtgata aaggacgttc agaaaaagga agtggtcttg gtctatctat
    13981 tgtcagtcaa atggtggagc gaatgggtgg tcagatttcc gtagaaagtg agattggaaa
    14041 gtataccata tttacagtga gcttaccatt aatataaaat tgtttttccc ccatgtgaca
    14101 tgtatacaca gggggatttt ttgtaagtta taaagttgca aggttaatac aaggttgttg
    14161 caaggttaat gtaagattga ggtggtatga taactttaat aaacaaagga ggcttttatt
    14221 atttatgaat aatttaatta ttgaaacaaa aaatctcact aagcaatatg gtgctcaaaa
    14281 gagcgtttct aatttgaata ttcacgttca gaaaggtcgc atttacggat tgctaggaag
    14341 aaacggtgcg ggtaagacga caactatgaa aatgttattg ggtctaacaa aaccaactac
    14401 tggagaaatt aaaatatttg gtcaggacat tcacaataac gagaagaagc ttcttcctcg
    14461 aattggatgt ttgattgaat caccagggtt ttatcctaat ctgacaggaa cagaaaatct
    14521 caaaatcttt gcaaggctta gaggaatacc aaaaaaaaat gcagtagaat cagcactgga
    14581 tgtagtcggt ttgccaaaca atgacaaaaa gttattttct caatactctc ttggtatgaa
    14641 acagaggttg gcgattgctc ttgccattat gcatgaccca caactgttga ttttggacga
    14701 gcctatcaat ggacttgatc ctattggtat tgcagaagta agaacgttta ttcgtgatct
    14761 ctgcacagaa cgaggtaaaa ctattttgat ttccagtcac attctttctg aaattgcctt
    14821 gttggcagat gacattggca ttattgacca tggtattttg ttggaggaag aaagtcttgc
    14881 ccaattagaa gaaaaaaaca gtaaatacat acattttgct gtatctgatg cagcacaggc
    14941 agcaaggctt ctggaaaccc aacttaacct gaaaaatttc aaagtagcag ataattacaa
    15001 tattatgcta tatgatacag atgtttctgt tgcagaaata aatcgtgctt tcattctaaa
    15061 caacattgag gtatcagaat ctcatttatg cgaagatact cttgaagatt acttcaagaa
    15121 agtaacaggg ggtgaaggaa ttgcttgata taatttcatg tgaattttca aaactcaagc
    15181 ggttaaaatt tatactgatt tctattttag gtgcttgctt atttccaatt cccgcaacaa
    15241 ttttgattgc aaaggacaac ttgccatttg agcaattgtt caaattagtt gtcaattttg
    15301 gatatttttt gctattacca attgtattaa gtattgttgc tagccaacta ttttttatag
    15361 aaagagataa tgatgtatta aaaaatcttg caacagtgcc agtgcaaaaa ggaaaactgg
    15421 ctctggcaaa attggctgtg ttgctgttta tatcattgtt ctactctgtt gcagggttag
    15481 gagctactat cattggaggt ttcattgttg gaatagtaga gggggttgct gtaaaattag
    15541 gaatgagcat tgcttttgga attatgtttt ttgttactgt acttcctgtt gtggtactca
    15601 ttgtatattt caatcgcagc tatatttttt ctattatact ttcttttgta tatgctattt
    15661 tcaattttag tatatcctta aatattatca attttgaacc aaataaccca atattaagtg
    15721 tattacctgc tcctgttata atgcgttggt ggatggcatt ttggggagat cccactggcg
    15781 aatacacggc acttagacag ccatatttgc tctcaactcc tgcttgtgta ggtattttat
    15841 gtttgataac cattatagca gtcctgctga tttgtatgat atacaaaaag caggagaatt
    15901 aggaggtatc tatgcttgat ataattacta cagaatttca aaaaataaaa agatataata
    15961 ttctatggat tggtgttgtt gctgttctgt tttcagcact attggcagtt tttcaggttg
    16021 taagttcaca cggcagtgac cctctaacgt atgagagctt tgcaaacgga gttatctgga
    16081 ataatttcag tcttagtttt ccatttggga ttacacttat tggaggatat cttattaacc
    16141 gtgaatatac agaccaaacc ttaaaaaaca tcctaactgt tcctatttca ttacgcaaat
    16201 tgctagtggg aaaattaatt gcggttggag gaattactgt attgttaagt cttttcagct
    16261 tcttatgcac atttattttg gggttgttat tttgtaatat caatatatct gttacactcg
    16321 ttgtaaaatc cttagttcag attttgtcta ttaatctgta ttgttatgtt gctgttttgc
    16381 cgattattgc ttattttggt cgtaaacaaa atgcatttct gacgggtgta gggattgcat
    16441 ttgtatacgg tttttgtggt gtgtttgttg ctggaagaaa tctgacagac ttttatccaa
    16501 tcacagcagg tcttggtcta gttggataca acaatggtgc tggtactgtt tatcagccac
    16561 ttatcggtgc tatgactttg attgtaattc taatcttgac aactattcta cttgtattta
    16621 cacctaatta tgataaagtt atgaccatat ctaagaaaaa agggataaag cataaaaagt
    16681 attctcataa ataacataaa tattgtttct attatttaag ttgctagaca gtgaaaagaa
    16741 aaaatctgtc gtacagtggc tatatgctca tgtattcaag aagctacagt tttgattttt
    16801 cataactgta gcttcttttt gtatttatta aatcacgact atcataatac ttgcaatagg
    16861 tatagcgaca agagagagta ctgtcatatg tgttttatat attacattaa gtatattcta
    16921 aaatatatgt tttaaataat atatttgaaa ataggaatat ttaaataaaa aaaataatat
    16981 tatatatagt tgacaaaaat tggcaaatga tataatatat aaatatagaa atagtaatca
    17041 ttactattat taaaaaggga ggaattaatt atgaacttaa aaggaactaa aacagaaaag
    17101 aatttaattg caggatttgc aggagagtca gaagcaagaa ataaatatac atactacgct
    17161 tcaaaagcaa gaaaagaagg ttataatcaa atagctgcta tatttgaaga aactgctaac
    17221 aatgaaaagg aacatgctaa attatggttt aaacttttac atgatggtat gccttcaaca
    17281 gaggaaaatt taaaggatgc agcagcaggt gaaaactatg aatggactga tatgtatgca
    17341 aaatttgcta aagaagctag agaagaagga tttgacaaga tagcttattt atttgaagct
    17401 gttggaaaaa tagagaaaga gcatgaagaa agatacttaa agcttttaga aaacttaaat
    17461 gaaggaaaaa tatttaaaag agatgaagaa gttgtttggc aatgtcaaaa ctgtggtcat
    17521 gtgtatgtag gtactgaagc accagaaaaa tgcccagtat gtgaccatcc aaaagcatat
    17581 tttaatataa aagctgaaaa ttattaaaaa ttatcattat taaaaaggaa tgttaactat
    17641 gaaattttct aaacaacgag aactgatttt aaatgaaata ttaaataatc cggttcatcc
    17701 tactgcggat tacctatatg aaaacttaaa aaaagataat ccaaatttaa gcttaggaac
    17761 tgtgtataga aatttagctc agctaacgga acatggcttt ataagaaagg ttagtattcc
    17821 agggtatcca gatagatttg atggtcgaat agataatcac tatcatatca tatgtgaagt
    17881 atgtggagag gtatacgatt tagaatccga agttcttaat aatttacaag aattaatatc
    17941 tgaggagaca gacataaaaa taacatctta taacataagt tttaaaggga tttgtaataa
    18001 ttgtaaaagg tgtagccaag taggttaatt ttcaaattct aaaaataatt aatgaggtga
    18061 gtttatgtgt agtgaacaaa aattttttat atgtgagact tgtggaaacc ttgtaggaat
    18121 gatacaaagt ggtggtgtac ctattttctg ttgtggaaaa cctatgaagg aacttgttcc
    18181 taatacaaca gatgcagcag ttgaaaaaca tgtacctgta attgaagtgg atggaaataa
    18241 tgttactgtt aaagtaagta gcacaactca tccaataact aaagaacatc atatagcatg
    18301 ggtgtatctt atgactgaac aaggtggtca acgcaaatgt ctagcagtag atggagaacc
    18361 tgtagttaaa tttgctttga atgatgatga taaagtgatt tctgcctatg cttactgtaa
    18421 tttacatgga ttatggaagg ctgaactgta atatttaaat aaattgagtc aaaaaggagg
    18481 ctataaatgt attttcatac aaatagcctc ctataaattt atataaattc aagttttaat
    18541 tttgtttaat gtcaatataa tataaggaga aaataatatg tcaatttata aatgtagtgt
    18601 ttgtggatat atttatgatg aatctaagaa tgataaaaca tgggatgaat taagtgaaga
    18661 ttgggaatgc cctgtatgta caaaaggtcg tagttatttt ggaaagataa gtactgttta
    18721 ctatgaagaa gatgaaaaga tagcagaaga catagttgaa gatgaatcta aattaaatac
    18781 tgaaaaagag ggagatttaa attacttaag tacatattta agaagagatg atgaagttga
    18841 gaaacatatg gatataattc acgaaatggc tgttacagga aaatctataa tagaacctat
    18901 gagaactaaa ctaccagtaa tttcttggga tgatatttta attatgggtg ctcaattaaa
    18961 tccattacct ttaaatgagc atgatgaagt aaatacaaca acaataatag gaaaaaaagc
    19021 aaagaaacct atgattatag aaaatccagt ttatatttcc cacatgtcat ttggagcttt
    19081 gtctaaagaa ctaaaaatag ctttagcaaa gggggcagcc caaaataaga cagctatgtg
    19141 tagtggtgaa ggaggtatat taccagaaga gaaggaagct tcatacaagt atatttttga
    19201 gtatgtacca aataaatata gtgtaactga agaaaatctt aaaaattctg atgctataga
    19261 gataaaaatt ggtcaaggaa caaaacctgg tatgggaggt catcttcctg gcgaaaaagt
    19321 aactgaagaa atagcaaaag taagaaataa gcctgtaggt caagatgtaa taagtccatc
    19381 ttgttttgag gaaatacaat ccaaagaaga tttaaagaaa cttgtagatg aacttagaga
    19441 agtttcagaa gggcgtccaa taggtgttaa aatttcagca ggtcatattg aaaaagatat
    19501 ggaatttata gcttatgcta aacctgattt tgtaactatt gatggtcgtg gtggagctac
    19561 tggagcaagt ccaaagttgc ttaaagatgc aacttcaatt ccaacaatat ttgctcttta
    19621 tcgtgctaga aaatacatag atacacatgg attagatata gatttggtta taacaggtgg
    19681 gcttcgtata tctactgatt ttgctaaggc aattgctatg ggggctgatg cagttgctat
    19741 tgcaagttct gctcttatgg cagcggcgtg tcaacaatat cgtatatgtg gttctggtaa
    19801 gtgtcctgta ggagtagcta ctcaggatga ggaattgaga aaaagattac atatagaaaa
    19861 tagtgctaat agagtggcaa actttttaaa tgtaagttta gaagaactta agacttttgc
    19921 tagaatttct ggtcataaag atatacatga tttaagtgtt gatgatttat atactgttaa
    19981 ctctgaagta tcaaattata caaacataca gcatgtatag attgaagttt gaaactatta
    20041 gattttagat agctataaaa gtaaatgata aatatatttt ataaaagaga gtgtcttaaa
    20101 atgaactttt tagttcataa gtcactcttt tttgtatgaa gccaaatata tttatcattt
    20161 tagcaatgaa aaagaatctt atctctttat tttgagatag actccttcta tttatgaaaa
    20221 ttaaagatat taaattgtaa aatattctgt gatgtcgtac tattaaacta aagattctat
    20281 actttaaagt aaataaaaat ttaaggagtg attattatgg aaagattgaa gatacaagtt
    20341 ttagtcgaca ataatactta tattgataga tactttgttg gagaaccagc agtaagctat
    20401 tatattgaga ttgatggaaa taggattttg tttgactcag ggtattcaga tgtattcata
    20461 tcaaacgctg aaaaattaaa tatagattta ggaaatttaa ctcatgttgt tttttctcat
    20521 gggcataatg accatacaag aggtatccag tttttagaaa ataggtatga tttatctaca
    20581 gttgaactta tttcacatcc taactgtttt atacctagaa aacatggaga gaaaagcata
    20641 ggagctccat tttctgctga ggaaattaaa aatattttta tgtataatcc taaggataag
    20701 ccatttaatc taagcaagaa ttgtgtgttt ttaggtgaaa taccttctat aaatgatttt
    20761 gaaaaaagag ctaaaattgg taaatgtaaa ataggagatt tgtgggaaga tgattatgtt
    20821 ttagatgatt ctgctatagt ttgtaaaact gataaaggta tttttattgt tacaggatgt
    20881 tctcatagtg gaatttgtaa tatagtagag tatgctaaaa aggtatgtgg agatgataga
    20941 gttattggta tactaggtgg ttttcatctt tttgaattga ataataggtt agatagtaca
    21001 atacaatatt tggataaaga agaaataaga atgttgtacc cttgtcactg tgtttctttt
    21061 aaggtaaaag cgaagatgaa tgaaatattg aatataaatg aagttggagt agggctgacc
    21121 ataagtatat agtatttgaa agtatataag atttaagttg aaggttaaaa agatattttc
    21181 tatagaaaat atcttttttg ttacgtttat tttaagagaa atcaatatac ttttatatga
    21241 aatattgtaa aaaaatttat ttttaatgag aatatattt
//
LOCUS       DAMKMI010000060        21093 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMKMI010000060 DAMKMI010000000
VERSION     DAMKMI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21093
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.061"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(196..1587)
                     /locus_tag="PC429_002026"
     CDS             complement(196..1587)
                     /locus_tag="PC429_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF3847531.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(1598..2818)
                     /locus_tag="PC429_002027"
     CDS             complement(1598..2818)
                     /locus_tag="PC429_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF3847532.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(2832..3620)
                     /locus_tag="PC429_002028"
     CDS             complement(2832..3620)
                     /locus_tag="PC429_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF3847533.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(3637..4911)
                     /gene="larA"
                     /locus_tag="PC429_002029"
     CDS             complement(3637..4911)
                     /gene="larA"
                     /locus_tag="PC429_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF3847534.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(5029..5742)
                     /locus_tag="PC429_002030"
     CDS             complement(5029..5742)
                     /locus_tag="PC429_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF3847535.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            5867..7126
                     /locus_tag="PC429_002031"
     CDS             5867..7126
                     /locus_tag="PC429_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF3847536.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            7406..7972
                     /locus_tag="PC429_002032"
     CDS             7406..7972
                     /locus_tag="PC429_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3847537.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(8173..9378)
                     /locus_tag="PC429_002033"
     CDS             complement(8173..9378)
                     /locus_tag="PC429_002033"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF3847538.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(9481..9587)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(9679..10047)
                     /locus_tag="PC429_002034"
     CDS             complement(9679..10047)
                     /locus_tag="PC429_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF3847539.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(10099..11376)
                     /gene="obgE"
                     /locus_tag="PC429_002035"
     CDS             complement(10099..11376)
                     /gene="obgE"
                     /locus_tag="PC429_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF3847540.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(11487..11777)
                     /gene="rpmA"
                     /locus_tag="PC429_002036"
     CDS             complement(11487..11777)
                     /gene="rpmA"
                     /locus_tag="PC429_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF3847541.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(11791..12108)
                     /locus_tag="PC429_002037"
     CDS             complement(11791..12108)
                     /locus_tag="PC429_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF3847542.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(12123..12434)
                     /gene="rplU"
                     /locus_tag="PC429_002038"
     CDS             complement(12123..12434)
                     /gene="rplU"
                     /locus_tag="PC429_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF3847543.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(12535..13956)
                     /locus_tag="PC429_002039"
     CDS             complement(12535..13956)
                     /locus_tag="PC429_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF3847544.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(13974..14675)
                     /locus_tag="PC429_002040"
     CDS             complement(13974..14675)
                     /locus_tag="PC429_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF3847545.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(14677..16521)
                     /locus_tag="PC429_002041"
     CDS             complement(14677..16521)
                     /locus_tag="PC429_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF3847546.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            16840..18033
                     /locus_tag="PC429_002042"
     CDS             16840..18033
                     /locus_tag="PC429_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF3847547.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(18245..19327)
                     /locus_tag="PC429_002043"
     CDS             complement(18245..19327)
                     /locus_tag="PC429_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF3847548.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(19891..20682)
                     /locus_tag="PC429_002044"
     CDS             complement(19891..20682)
                     /locus_tag="PC429_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847549.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(20686..21015)
                     /locus_tag="PC429_002045"
     CDS             complement(20686..21015)
                     /locus_tag="PC429_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847550.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         6645 a         3814 c         2346 g         8288 t
ORIGIN      
        1 ttactttatt tatgtacaca tgcttttagg atgacaacat caacttaaat attatgttac
       61 tttctaaatt taagtaaata ttgccatcct aaaaatttta caatttagct tcagatttaa
      121 aagtaaagtt taaaatatta tatttaaatc taatttaata ttttttgttt tgtaaatatt
      181 attaaattaa tatttctaat atattttccc tggatttaaa atatttttag ggtcaaaagc
      241 taactttata tttttaatta acatcatata agcatctgaa tttgattcat gtaagtactc
      301 ttttttagca tatcctatgc catgttcacc agaaacttga ccactaagtt ctctagattt
      361 tttatacata tagtcaaaag tttcttttaa tcttattttc catgtattat catccatacc
      421 atcttttaaa atatatatgt gtagatttcc atctcctgca tgaccaaaac tttttattct
      481 tatttttaat ttgtcttgaa gctcgtgagt gtatcttata aactctgcta tcttatctct
      541 tggaacaacg acatcacatt catccatttg tgtcgtagat gcctttatag cttctaaaaa
      601 tgcacctctg gctgaccata tagaatcgtt tctttcttgt gtatctgata taaatacatc
      661 taatgcacca ttttctaaac ataaattagc tactttttca tattcttttt ctatatcttc
      721 agtgctattt ccatcaaatg taagcaaaag ataagcatct gatgtattat ctggaaactt
      781 tttacctaaa aattcttctg ctgccaatat cacatctctt tccataaatt ctatagccgt
      841 tggtattgat tttgatttta ttattttagg aacagtttct attgccattg ataaatctgg
      901 aaatggtata agcaaactta tacttttctt tggtaaagga agcaatttta atatagcttt
      961 agtaactatt cctaaagtac cttctgagcc cactaataaa tcctttatac tatatccaga
     1021 actattttta accacctttc cacctacatt tataatttct ccatttggta gtacaacttc
     1081 aagacctctt acataatccc ttgtaacgcc atattttact gctctcatac cacctgcatt
     1141 agtgtttata ttacctgcaa ttgtagcact tttttcacct gggtcaggtg ggtaaaataa
     1201 atctctatct tgaacatatt gacctattgt catcaataaa acacctggtt ctactgtcaa
     1261 tgttaaattt tcataatcaa cttcaagtat tttattcatt ttacatagat taatcattat
     1321 tccaccattt atggcaacag cagcacctac aagaccagta ccttgacctc ttggtgtgac
     1381 tggtatatta tttttatatg cgtatttcat tattttagat acttgttcag tttcaagtac
     1441 atttactaaa acttcagggt acttctcaat ccctccaagt tcatcatgtg aaaaatcttc
     1501 atttatatca cttccaacta atacattttc ttctccacaa gtatctatta aaaaatctat
     1561 atctttttta tcgattaatt tatacatttc tatcccccta tttagctgct gatgccatat
     1621 atttttgatt attatctttg ttattttcta ttttttcaat caatttagga ataatatcat
     1681 aaatatcacc tattaaagct aaatgtgcta catcaagtat aggcgcttta tcatctttat
     1741 ttattgctac tatatagtca gcaccctgca tccctgcaac aaattgaaca gctccagata
     1801 caccacaagt tattatcaat ttaggtttta cagttctacc actaagacct atttgttttt
     1861 tagcatctat ccaacctgct tctattacag gtcttgttcc tgccacttga gcgtttaact
     1921 tatcagctaa tttatatatc atctccatat cttcttgttt ttttattgct ctacttgcca
     1981 caactatcac ttcagcttct tccagaccaa cttctttatt tttttctttt acactcagta
     2041 ccttaatttt agattttagt tctttttttt ctactttaca taaagttatc tttcctgtag
     2101 tattctctat tttttctggt gctgaaaata ttttatatct tacagtggca aattgtggtc
     2161 tattattagg tgtgtttata tgagccatta tatttccacc aaatgctggt cttatttggt
     2221 ctaaatcagt atttgattga atatcaagta ttgtacaatc tgctgtaaga cctgttctaa
     2281 atctagcggc aagtcttgga gctaatgacc tacctaatgt agttccacca actaatacta
     2341 tcgttggttt tattttatct ataaagtttt ctattgcttt tgaatatggt tctatcctaa
     2401 aatctttaaa ttcatcttca tcatatacaa atacttcatc caccccataa gaaatcaaat
     2461 tactgcactt atcttttatg tcttttccta caaatacaca atacacaggt tgttttattt
     2521 ttgatgccat ctcacgagct ttacctataa gctcgtacgt tacaggatgt atatttccat
     2581 tatgatgttc aacatatact gcaattcccc tccattcatc tttattaatt tgtactttac
     2641 tactctctat aaattcaaat gcgccttttg gtccactttt gacacacaac ttgcacatct
     2701 tacagccagc acttatttct actatacctg acttttcttc tatagcccca aaaggacata
     2761 tttctaaaat tctctttaag tcagttattt tatcttgatt tacaactatc tttgacattt
     2821 attactcccc cttacaccaa ctttaattcc tctaatttat ttacaagtgc aaaacttagt
     2881 tcatctgcat ttcctcttac aatattttta tctgtatttg gctttggagg aaatattctt
     2941 acaacttgag taggagaacc attaagtcca tagttatttt catttttatc ttcaaagtca
     3001 tctaagctat atacaggtat ttctctatct ttagtattaa gttttaattt aaatgaaggt
     3061 agtcttggtt gaaaaatgtc tttttctaca gttattaaac atggatattc aatctcacat
     3121 acttgtaaat catttggcat atctatttca acaactatac tttcatcttt aacttcaatt
     3181 aattttgtaa cgttagtaac atgaggtata tctaaaaatt cagctacttc aggtcccacc
     3241 tgtgctgtat caccatcagt agtttgtttt ccacaaatta ttaggtctac ctttcccatc
     3301 ttcttaatac cttgtgatat ggtataggaa gtagccaata catctgctcc tccaaatctc
     3361 ctatcactta ttaatgctcc ttcatcagct cccatcataa aactttcttc aattaccttc
     3421 tttgcctgag gaggacccat gctaagtact tttagattcg ctttcttatc ttcctttatt
     3481 ctaagagctg tttctaaagc atataggtca tagggattca tttttgaatc tactccatct
     3541 ctttttagta ctcctgtagt ttcatcaact tctactgaag tcgttccagg aacttgttta
     3601 atacatgtca aaatttccat aaagtacact ccttttttat ttaactataa ctgatactcc
     3661 atcaggtata actactactt tgctctcctt accaactata ctattcgcca tttctaaagc
     3721 ttctttaaag ttaaatgcat gtttcatatg catttttttt atcatttcag gattacactt
     3781 atctgttacc attatcacag tatatttaga taatattctc gctagtatct gaaattccca
     3841 ttggtctgga atcgtatcta ctctatcgac acttctaatt ttatctaaaa gttgttttgg
     3901 agaatctgca tttgctacat tttcataaaa agattgacct ccatgaccat cattacatgc
     3961 tgaaatcatt attattactc caccgtcttt acaagtagct tcagctgctg tcattccttt
     4021 aacagattga taaatatttt ggtctagtgg atatccacca tttgtagtta caacaatatc
     4081 agattttata gaatttactt ttgataactc tgttacaaat ctacatcctt tagtatgtgc
     4141 tttttcgaca tcaccagcaa aggaagcaat tattttttta tctttatcta ttacaacatt
     4201 caatatgaat gctaattttg caactttagc tgcatatacc atatcctcat gaataggatt
     4261 attattcaat tttccagttc ttgaattatc actatctata aagtcactac agtgattgta
     4321 cattatagtc tttgcagatg cgattcctgg aagtacgctt tttctgccac ctgaaaaacc
     4381 tgcaaagaaa tgaggttcta taaaaccttc tgctatcaaa agttctgcct catatgctag
     4441 tttgtttata tataaatctc cacctgaagg caatatccct actttacaca tagatttatc
     4501 atcagtagat atatgcataa ctatatcttc attatctact atttcttctc ccattttata
     4561 aattagctct tcccttgtac ttggcctatg aaatccagtt gccactataa tcttcacatc
     4621 aatttctgga ttaacattcc taatccttct taatataatt ggcatagtta ttctacttgg
     4681 tacaggtctt gtatggtcac ttgtaattat gactacattc tttttcccct taaccagttc
     4741 ttccagagaa ttagagccta taggattatt catagattct tcaactatct cttcttgagt
     4801 cttatgttct atataattat ccgattttga ctccaaaaca ccgagaaaac gttcatcagg
     4861 tatttctaaa atcattcctt gttttgaata cggtactttt aactttacca tttttaatct
     4921 cccattataa taaactataa aattggtatg tggtcatacc agttttacat ttatataata
     4981 gtaccattat ctaccatttg caagaataat ttattttata acaaactttt aattttcatt
     5041 ttttctataa tttttactta taaaatccat atgttccttt gctttctcac atgcaagttc
     5101 tacatcattt tctacaactg cattgtatat agcttcatgc tgtttaagca atatattttc
     5161 attaaaaggt tctgcgatta ttttttctct tgcatcttct ataaatccat caaataattt
     5221 tgatgctgtc ataaaaagac tttgaattaa ataattttta cttaatgttg ctagtttact
     5281 gtgaaatttt tggtctaata ataccagtac tcgattatta tcttttttat tagtttctac
     5341 cctcatttca tctattatat gtttcaattc tataacttca gaagaagtta ttctctttga
     5401 agcaagtttt gcaatctcaa tttctaggat tattctaagc tctaatatat cttcaagtgt
     5461 accattatta agtttaaaaa tcatagataa aggttctatt aaagtttttt ctatatttgt
     5521 acatataaaa tttccttcac cctgtctgct ttctatcact cccatagtct ctaataccct
     5581 aatagcttcc cttatggaag ttctactcac attcatttgt tcagacagtt ctctttctga
     5641 tagtaattta tccccctttt ttaactctcc atttagtatt ttatattgta tttgctcaat
     5701 tacttgttca taaacttttt tactagctat actattaaac attttttcct ccaatatgat
     5761 aatcgttgtt gtaatatata tctattatat atcttaagcc attacattca tatattttta
     5821 ttacttttaa tataatatct tataactcaa atttacaggt gatattatga aaaaaaataa
     5881 aattattgct ttttttgctc catctatcat agttggagtt ctagttttag ggattatcct
     5941 atacccaaat gaagctataa aatctgcaag agaaggcttt tctatatggt ctaatgtttt
     6001 agttccatca ctactaccat ttataattgg agcaaactta attgttgatt taaaaatagt
     6061 ggatattata gggtttatca tcaatcccat aactagattt gtatttaatg tttcaggaaa
     6121 gagcgcttta gcttttgcta tatctacagt atctggttat ccagtaggag taagtttagc
     6181 ttctgaattt aggagtaatg gacaaatttc taagtatgaa gcacaaagac ttgtgtcttt
     6241 ttgttctaca tcaggacctt tattcataat aggttcggtt ggtacaggta tgttccaaaa
     6301 ttcaaactta ggttatttaa tgattctctg ccattatcta ggaactatat tggttggtct
     6361 tttctttaga aattatggta atgaaaacct tccaaagaca aaattggatt taaaaacaaa
     6421 tataaaaaat gtaatcaaca ttagaaattc aagtgggaat ggtttttttg ttttatttgg
     6481 aaatgctgta tttaatggag taaacacatt acttacagtt ggtggatttg taattgtttt
     6541 ttcagtagta tttaaaatac tatctctttt taatgtaatc tcattaatag catcagttat
     6601 atatttgcct ctttcatttt taggtgttag taaagaactc tgtcaagcat ttgtaagtgg
     6661 tctatttgaa attactatag gatgcaataa agtatctagt ataacaagtt ctccagaaat
     6721 tttaagagca tctttagcta gttttttgat aggatttagt ggattatcca tcctagctca
     6781 atgttgtaca tttctatcaa aaacggacat agatttaaaa ttatacatac taagtaaatt
     6841 tgcgcatgga gttttagctg ctatctttat atttatactt tatccaattg caaactctgc
     6901 tgtgttggtt tctagctttg ctgatacata caacgttata tataataatt taatctggtt
     6961 ttattacctt agctattatg atacaattct tcaaattgta gtaataattt atctaatctc
     7021 agccgtattt attgcaaaaa aacatatgaa aactttatct acaaatggga aactaatgcg
     7081 atataaaaat acttttttca ctaagaaatt ttcacgaaaa aaataaaact aaaaataaaa
     7141 ctaataaaac ttaactaaat acttccattt ttttattata tttgtcataa ttttaataac
     7201 ctttttaatt agtttaacag catagttatt tcatcacaat ttaataattt tattagaaat
     7261 atttttcaaa aaattctcct taatcacata tatcacactt gtataagttt actgggaaat
     7321 ttttccatta tataatattt catttatatt atataataaa taccgaccta aacttaccaa
     7381 atcaaactaa gaggagtggt tgaaaatggc aacaagacct atagaaatag acgaatataa
     7441 aaagataatg gaactattac atactggctt tacttatagt gaaaatggcg ttgaaaaaag
     7501 atttagaaaa aatcctaaag ttgcattggc actaatgtta gaagctaatt taggtctaag
     7561 aatatcagat attttaagat taaagatagg taattttaaa ggtaatatgt tggaaataaa
     7621 tgaaaaaaag acgggcaaat tacagtatag accagttaat aaaaatataa ttgagtccat
     7681 aaatgaacat gcaagaaagt ataatcttaa atcaaatgac tacttagtaa atataaagac
     7741 aaaggctatt caaaaacagt tgaggataat ttgtaaacac ttaaatctgt ataatataag
     7801 tacacatagc tttagaaagc tctatgctac tactcaattt gaaaagagta acaataattt
     7861 agagttggtt aaagaacttt taaatcacag ctctgtagcc actacacagc gatatataag
     7921 ggtaactcag caagctatta atgaagctag tgaaaatttc tttattggtt aattataacg
     7981 tatacatttt tctattatct tattaattca ccagatatta aaaaagagtg tctcaaaatg
     8041 aactttttag ttcataagac actctttttt tataaaatca aatatatttt tattatttca
     8101 acactggaaa aaaggcttat ctctctattt tgagacaccc tattaaattt tcataattct
     8161 tgcataaaca tattaatcta tcttcaataa ttttttagct gattcactgt aattatttct
     8221 ttttacaatt tccaaaactc tatcaatctc atgttctgac tttgcactta caaaaatcgc
     8281 caattcttca tcatctataa tatcataact atctacatat ttatctaaac attctaaaat
     8341 tccatcttta ttattatacc caccaaatct aactactgca ctacactcat cttctataag
     8401 tccacttaaa tccaatttcc cttttgaaaa agactttata tttgaacttc tattatctat
     8461 tatatctata atatctgcat aaacagcact tcctgttgca cctcttccag ctcccttacc
     8521 tacaaaagaa agttcaccta ctgaatcacc tactaaaata actacgttga actcattatc
     8581 tatctttgaa agcatattat tattgtccac taatgctggt ctaacaaatc cacttacttc
     8641 tcctgattca tacttacttc tagcaaccaa ttttatctta caatttaatt tttttgcata
     8701 ttctatatct ttttcatcta ttgaagttat tccttctaat aataaatctt tccaatatac
     8761 tatttttcca taagataaag tagacaatat agataacttt ctagcactat catatcctaa
     8821 aacatctgca tctggattaa cttctgcaaa tcccaactct tgtgcctgtt ttaaagcgac
     8881 atcatacggt aaattttcat catacatctt tgataatata aaattacaag tcccatttaa
     8941 aatagcgttt atgctcttta tattgttgcc ttccagtgat tctatcaatg gttttaaaac
     9001 tggaatacca cctgccacag atgcttcaaa cttgatgcta acattattta agttagctaa
     9061 ttctataagg tcactacctt tttcagccag taaatcttta ttagctgtca ctacatgtat
     9121 ttttttattt aacgctcttg ttacataatc aaaagctgga tttaatcctc caattacttc
     9181 aactagtata tcaatatcct tgtcaaatat atcttctata tcagtagtta tcttatcaaa
     9241 gtacttctta tacttgtgtt tttctttatt tttaactaaa attccaacaa cttctatatc
     9301 tctttttgaa gtattatttt ctattaaatc aattaatcca cttcctactg ttccataccc
     9361 taatattgct atcttcactg taacataccc ccatcatttt gattatatta aattatttat
     9421 atatataaat ataccaataa aaaaagcttg agtataaact caagccatat gacgggttta
     9481 tactcatctt tcagcataaa tgctgcagga tttagcacca atgtataatt aaatatactg
     9541 gttgccgggc ttcacagggc cagtccctct gccactcttg ataagattgc taatttaaat
     9601 ttatgtttaa tagtataata caatttcttc caattgtcac tattttttta acttatttcc
     9661 ctaaaaggat ataaactatt atctaacagc tcttttagtt ggtttaccct tttttgtaac
     9721 attattattt ttagtctttg ctttcgcctg ttcatgtcct ggaaaaagta ttttaggctc
     9781 ttcaatattc ctcttgtaaa tagtaaattt aaaaccaata gcctgcacaa attcagctct
     9841 taaagcttca catactgcat tagcagtttc tttagtatca agacctgcat tttctaatat
     9901 agtaactttt actaattctc tagttcttaa cataccatct aattgctcta agaaactctc
     9961 tgtaacacct tctttaccaa tttgtgtagt aggctttaaa gtatttgcca aagatcttaa
    10021 atacgccctt tgttttcctt ttaacatatt ctcacctcta taattctata cttaaattat
    10081 ctataatcta tatttaaatt aattgtaaaa ctcaaattct aattcataaa ttttaaccac
    10141 atctccatct tctattccca tttctctaag cctatcaaaa acaccttgac tctccatagc
    10201 tttttggaaa tattgaaggg attccatatc atcaaaattt acagaataca ttattcttct
    10261 aagtgcttta cctgtaacta catacacacc atcttcaata tctatactta actcttcttc
    10321 agtaccaata tcaagttctg gtctatacat ttcttcttct gaaactagct ccacatcttc
    10381 tacttctttt agcattttag aaacataggc tattacttca tctatgcctt gacgagttgc
    10441 tgctgacatt ttaaatactg tatatcctct gccttctaat tcactcttaa acttctcaaa
    10501 cttactctca tcttctaata tatcaaattt atttgcaact acaacttgtg gtctctttga
    10561 aagcttttca ttgtacaatt taagctcatc atttatcttg tcaaaatctt caatagggtc
    10621 tcttccctct aatccagata tatctacaat atgtatcaat acctttgttc tctcaacatg
    10681 tcttaagaac tcatgaccta atcctatgcc ttcagaagca ccctctataa ttccaggtat
    10741 atctgcaagt acaaaactat ctccaaactt agtttgaaca acacctagat ttggtgtaag
    10801 tgttgtaaaa tgatagtttg caattttggg tttagcttta gttacaactg atagaaatgt
    10861 tgatttacct acatttggaa atcctaaaag accaacatct gctatcattt taagttcaag
    10921 agttatccat ctttcttctc catctgtacc agattttgca aaagctggag cttgtcttac
    10981 tgcatttgca aagtgttggt tacctttacc acctatacca ccttttgcaa ctatagctgt
    11041 atctccttct ttttttaaat ctgctaatac aagacctgta gcctcatctc ttattactgt
    11101 acctgctggt acttttaata ctaaattttc tccattttta ccagctcttt ttcctttgct
    11161 tccgtcgcca ccattttgag cttgatattt tttctggtac ttaaagtcca ttagagttct
    11221 aagccccaaa tcaacttcaa atatgatgct tgctccacga ccaccatctc caccgtctgg
    11281 accaccagct ggaacgtatt tttctcttct aaatgctacc gaaccatttc caccatttcc
    11341 agccttaaca aatattcttg ccttatctat aaacaaattc ttcacctact taattaaaaa
    11401 cttttaatta gcattaccaa aaataccatt ataaattcat cagtattata agaaaaagga
    11461 gtgcctcaac actcctttta tgtatattac tcagctatag ctactggata tatactaact
    11521 ttttttctct tcttgtcttt tctttcgaat ttaacagtac catcaactaa tgcaaataaa
    11581 gtatcatcac taccttttcc aacattagtt cctggatgta ttttagttcc tctttgtcta
    11641 actattatac ttccagcagt tactaattga ccatcgaatc tcttaacacc taatctttta
    11701 gatatagaat ctctaccatt tttagaagaa cctacccctt ttttagatgc taataattgt
    11761 aaattcatgt ttaacatatg gagtcacccc ctactttttt aaaattttta tattttttgg
    11821 gtattcccgt ttaatttctt taaccgctaa ttgaaagtga tttagtaata attgtgcgtc
    11881 ttttacatgt gtatctttga ctatacactc tatatgacct tcttctccta ctactttttc
    11941 aaattcaatc ttttgtaatt tgtctaaaga atttataact gcaatagcat tagatgtaac
    12001 tgcagcacat actatatcat agcctatttc agcaaaatca gcatgtccct ttaaacaaaa
    12061 accttgcaac aagaaatcat cattataata atatttaact tttatcataa cttaaccaat
    12121 aactaagcgt ttattttttc tacaactatc ttagtgtatg attgacgatg tccttgtttt
    12181 cttctgtaat ctttttttgc tttatacttg aaaactataa ctttcttagc tttaccttgt
    12241 tcaacaactt tagcttgaac agatgctcct tctactactg gagaacctaa ttttaattct
    12301 ccatctttag agcaagctaa tacttcgttt aaagtaacaa catcacctgc gttagcttct
    12361 aatttttcaa cgaataatac atcaccttca gaaactttat attgctttcc accagtcttt
    12421 actatagcgt acattaaata cacctcctcg gcctcaaact cgccagtaca aggtacttca
    12481 tttgaagatt taaaccttta ctgtgcggca ttacaattat taattataaa atatttacat
    12541 tttattgtca actattttat cgaaaataac attggtttta ttgtgcttta atgtactatt
    12601 tgcgtttaaa gaaatcttta tattatattt ttcaccaatt ttatcaatta tatccatata
    12661 atattctttt atttgttcaa aaataaaatc attaaattct accattatgt ttttgtaaac
    12721 agtatgttca cttattctca taatttcttt ttcaatacta tctagtatat aatgtacaga
    12781 ttttattgtt tgttcaccat cacaacatgg acaagacatt aaataatact tatcaatgga
    12841 ttctttttct ctacgcctag caacttctac taaaccaagt tttgtcatac ctaatacttc
    12901 agcttttctt ttatctttat taaattcttc ttctaggatg tgtaaaatct ccttttgatg
    12961 cttcttttta tgcatatcaa taaaatcgat tattattatc ccagctatgt ctctaattct
    13021 aagctgtctt acaatttctt ttgcagcttc aagattagtt ttataaacgg tttcatcaag
    13081 tttaccagtt ccaataaact tacccgtatt tacatctata accgtaagag cttctgtttt
    13141 ttcaattatt aaataacctc cactctttaa ccatactttt ttatttaata atttttctat
    13201 tttagattct attctgtata aatcaaatac atctctattt tcctcaagac acagtttatc
    13261 aacataagtc ttatctatac ctcttaaaat agacttaagt tcagagtact tatcataact
    13321 attggtaatt atagattcaa tatcatcatt cacattatct tttacatatt tagttgcaaa
    13381 atctaaagac ttatataata atttcggacc tataccaagt ttatattctt ttaaaatgtt
    13441 ttcatacttt aatttcaact cttctatatc tttctttatt tcatcatgag tgcaaccttg
    13501 agcctcagtc cttattatta atcctagatt ttccttattt actgccttag taattttttt
    13561 taatctaaat cgttcttttt catcagtaat tttatttgat attgtagttc tatcattgga
    13621 aggtatatag acaatatacc ttccagaaat actaatctca gtgtttaatt ttgcgccttt
    13681 agttcctatt tcttctttat ttatctgaac caaaatatcc tgaccagatt ttatagtact
    13741 tccctttttt aattgtaaat aagcaagttt cttaaatcca atatctacaa aacaagcttc
    13801 tattcctggt ataacttttt ttacgattcc acgatatata ttggatacag tcttcttatt
    13861 taaattgtct tcaacaaaaa gttctgttaa tctttcatct tcaagtactg ctgttttctg
    13921 ggaccctatt agggactcaa taactatctt tttcacaact tcacctctcg agcttacata
    13981 ggagtcacta attccccatc ttctaataca tataaatctc ttctcaatat atcaacatct
    14041 aatgggtcaa tatcaagttc aaacatctct aatatctttg gtataagtat attagtattt
    14101 aaattagttt ttgagcctgt agctatagta gcagtaagag taatatgttc atcttctaaa
    14161 tttaaaacat caaaggttct tatcataggt cttatatcta cttcaaccat cttacctttt
    14221 ttattctttt ttgttatgat tatttcttta ttattcataa agtcaattac ttttcttttt
    14281 acaaactctt tcgtcatagg tcttttcaaa tctatattaa acaaatattc tccatagtct
    14341 atcacagaag aaagagaagg tgtttgtcta tcaatttctt ttgctttgat aaaatctatc
    14401 ccatctggaa gttgaattga tactttattt aaaaattcct ctactgataa atcatcttct
    14461 tctatttcaa tatctacata ctcaccttga ctttctgtcc ctaaagccaa agcattgcca
    14521 taactcattt tagggtgagg attaaatcct tgagaatgtg ataagtttat ttcagctctt
    14581 ctaaacgctc tttgtaacaa tctctgaagg tcaagatgtg atatatatat catgtcgcct
    14641 tcctttttaa attttactct tattatctta ctcatattag caaagccccc ttcctatatc
    14701 atgtatattt attccacaac cattgcagtt atgtctacaa tcagaagtga ttttttcttt
    14761 tttagcattt tcgtcttctc taattaagaa ttttttacta atacctacat ctatatggtc
    14821 ccaaggaaat acctcttcat agtttctatt tctatgtgca taaaaatcta tagaaagatt
    14881 attcttttcc atagcttctt tccatatatc taaattaaaa tgttctgccc atccatcaaa
    14941 tttagcacct agtctaaatg catcatatat aacttttcct attcttctat cgcctctagc
    15001 tactactgct tccatcaaac tagtctttga atcatgataa ttgtatttta tattgttatt
    15061 tcttaattta tttactaaat gtctctgctt attaataact tcttctgtag tatcttgacc
    15121 atgccattgg aaaggtgtaa atggctttgg tacaaatgta gaagtactca ctgtaacact
    15181 aaaacttctc tttagctttc ctccatttac ttttctataa atatcaataa ctttataagc
    15241 taatttagct ataccatcta aatcttcata agtctcagtt ggtaaaccaa tcataaaata
    15301 caacttaaca ctattccacc ccatctcaaa agctctttct gttgcatttt ccaaatcttc
    15361 ttctgtaaca cctttattta taacatctct aagtctttga gttcctgctt ctggagcaaa
    15421 agtaagtcca gattttctta cttgttgtat tttatcagca atttccattg aaaaattatc
    15481 taatctaagg gatggaagcg aaatcccaat attattagat gcatattcat ctaccaaata
    15541 atcagtcaac tctgataact tactataatc actcgtacta agtgatgata atgatatttc
    15601 atcatatcca gtattcttaa ctaatttatc taatatttct ttaagtcttt taacactctt
    15661 ttctcttatt ggtctgtata tcatacctgc ttgacagaat ctacatcctc tagtacatcc
    15721 tctaaataat tctagtacta ttctatcatg aacagtatct atataaggta ctattagttt
    15781 ttctggataa tctacagtct caacatcttt tattatcctt ttagtaggat ttttagtaat
    15841 cccttctcta ttaggagtta cactttgaac tgtaccatct tcattgtact tgacatcata
    15901 aaaacttggt atgtatactc cttctatgct agatatttta tataaaaaat cttctctagt
    15961 cgttttattt ttcttccatt ctttatactc atttactact tctaaattaa cctcttcacc
    16021 ttcacctaat attactacat ctacaaaatc tgctaatggt tcggaattat aagcacaagg
    16081 accaccaacc attataaatg ggtcttccaa agttctttct tcttttaata ttggtatatt
    16141 ggctaaatct aatatattta agatattagt gtatgaaagt tcatattgaa gtgtaaaagt
    16201 gacaaaatca aagtttgtta taggctctct tgactctaat gcaaataatg gtatactttt
    16261 ttctctcatt atattttcca tatcaactgc aggagaatac actctctcac aaaatacatc
    16321 ttcatcctta tttattacat catataatat atgactccct aagtgactca taccaacttc
    16381 atataaatct ggaaagcaat gtgcatatct tatcaattca ctattactag tatctttatg
    16441 aattgaattt atttcatttc caagatatct agcaggcttt tctacttttt ttaagatttt
    16501 tttcaagtct actttattca tttattaaaa ttcctccaaa caagctcttt atttttattc
    16561 ttatttataa gtaatattat acacgataaa atattttcta tcaaaaatat aactaaaata
    16621 ttgaatttaa aatacatatt attcacaaaa tagaatagct aaaaatattt aagaatattt
    16681 tctattaaat ttttattgaa atacaatttt tttacaaagt atgatatatt ataaacataa
    16741 aactattgtt ttacatgact tatcatgaca tcttttgttt cattcacctc aaaatggggt
    16801 atgttactta tataatgata aattttagga gggttaaaaa tgagtaaagt ttttgaagta
    16861 aaaaaagata tctactttac tggagtagta gatgaaggtt taaaagtatt cgacataata
    16921 atggaaactg agtttgggac aacttacaac tcttatttaa tcaaggacga aaaaacggtt
    16981 ttatttgaca ctgtaaaggc aaattttaaa gatgagtttt taagtaattt atcagaggtt
    17041 actgatatag ctaaaattga ctatgttgta attcatcaca cagaaccaga ccatgcaggt
    17101 agtttaaaat acttattaga tataaatcct aatatagaag tttattgtac taaagctgct
    17161 aaactatact tagatggtca aataaataga ccttttaact gtcatgtaat aaaagatggt
    17221 gaaattttaa atatcggtaa aagaaatctt agatttataa ctgctccatt cttacattgg
    17281 gttgatacaa tgtttactta cattgaggag gacaagactc ttttaacttg tgatgcattt
    17341 ggttgtcact ttgcaagtgt tgatgcagag gtagtgaata gtgaagatta tctaaaatca
    17401 gctaaacact actatgattg tatagttaag ccatttgcta aacatgtttt aagtgctgta
    17461 gataaggtgg taggactaaa catagaattt gatactatat tgacttctca tggtccaatg
    17521 cttactaaag acccaatggc tgctgttaaa agatatgttg agtggtctac agaagctgtt
    17581 aatacaacta atcaaaatca agtttctata ttctacttat cagcttatag taatacttta
    17641 gagatggcta aaaagataaa agaaggactt gataaagaag gtgcaaaggc tgaattatat
    17701 gatttagaag atatgacatt aacagaaatg cacgatacat tagtagtgtc aaaagtaata
    17761 ttactaggtt ctccaactat aaacaaaact atggttaaac ctatgtggga tttattctct
    17821 gttatagacc caatggctaa ccaaggcaag atagcaggag tatttggttc atttggttgg
    17881 agtggtgaag gtataactat ggctgagact ttacttaaat ctatgtcatt caaaatgcct
    17941 gtagaatctc ttaagaaaaa attcttccct agtgaagaga cattaaaaga gtgtatggca
    18001 tttggtgcag aatttgcaaa attagttaaa tagccattaa tcaaacataa tattatttaa
    18061 aataaatgat tttatattta aaaataaatt aaaatggagt tgtctcaaaa ttaatttttc
    18121 taatctaatt ttaagttcaa ctcctttttt attttctaaa atgactgata taatgataaa
    18181 tgcacccttt gtgcgagggt gcattattta taatatgttc tatttgtaca ctagtatatt
    18241 tgtattaatt cacatatcta gcgtatgcta ccatccctct tccagaatta gatgccgttt
    18301 caccaatttc cactttaata attacattat tttctttaca cttttttgat aattctgctg
    18361 cagaaaggtt cttaccagta aaatctaaag aaaatggctt atcatattca aaaggatact
    18421 catcaggtac accatctgga gctaatgcaa ccataccata tggtgtattc ataaacccta
    18481 ttacagtaac tgtatgcttt gatgggtcta aatttgaatt tttgcttgta aacttaattg
    18541 tatttattga tgagttgatt aaactacacg gaactccctc atacatttca atttttggac
    18601 gtagattatt tccataaact tcatcaagta ttgccccttg ttgatctgct atcatagtgt
    18661 gattcccagg tccaactggc gcagaaggct tagctggcgt aggtttctta acagttggcg
    18721 ttggattctg tgtctttact acttcgccaa cagctggata tgctttcttg tcaaatggta
    18781 atgggtgagt aagactcttg tatatgatgt ctgctcctgc tgatttgttg gcatcaatat
    18841 ctacatgata caaacgtcca tcctcaaaac gaataagatt ccactgatgg tcatttgttg
    18901 tactatgtac ataataacat tcaattccca tagcatcaca taaagctttc aagctcctac
    18961 acattccaga acaagcacct tttttagtaa ccaatgtata agcagtattg gctccctctg
    19021 ctgcgttcca gttaacatat gttgtgttgt ttttaatgtg attataagca accctaatct
    19081 tagcatctgt ggtcatgttc tttgtaattt tcttgttcat aaagtctgct gttgcttgtg
    19141 ctaccgaatt aagttgagct tgtgataact ttggaccata tatactgttg gttccagcta
    19201 cataactttt cggtgtcttt gcagtaacat ctttagctgt cataccataa actgttacac
    19261 tcatacatga ggttaaaacc atcattgcaa tcaatagttt agtagctaat ttcctgattt
    19321 ttttcatatt agcattctcc tttataatat attttacttt caattcccta ccattaatta
    19381 agttcatttt agatttaaaa tatggattat caacatgtat gcatagcaat tcacgtggtc
    19441 atagaatatt tagatttctt ggaaaatctg ttctatattt cctatatagt tcgtatacaa
    19501 tccaactcac aaacctgcat ttttgatatc atcacaataa aatgataata attaatatat
    19561 tcaaaaatgt tggtctgtaa caggtatgtc aattttccaa ttattggtaa tggcgagagt
    19621 gaggagaatt gtcaataata catttaaaat taaaattgta tacacttcat acattctccc
    19681 ctaacaacat tatacagatt ataatttgta tattcaatac attttttgga aagtataatt
    19741 atatcaaaat atgtgattat gtatagatac aatccactat ggagaaatat aaaagaaaag
    19801 agtataacaa acaataaaaa ataagatgat tcaaatttta ttttgaaaca tcttatttac
    19861 tctacaaatt aagattattt ggtgaattat ttaattatta ttttttatta aagaaatttt
    19921 aaatcttaat ataaaatacg ctattataca taataatcca aatgcaaatg ctactggaat
    19981 taacatctcc gattcaaaat aaaagaaata ttctaagaaa atatctggat ttactatatt
    20041 cccattcttg tctaaaaaat cttttccatt atatcctaaa tgctgtataa aattacttat
    20101 gttattataa tctgcttccc tctcatatac catattagca ttcaatccag aagaacttaa
    20161 attagtgttt ccatctttca tatctattac atatagtcta agagctttga ctttactata
    20221 cttatcttta tcaagtattt tttcaagttc taacttatat tcaccaatct ttgtcatatc
    20281 atgtctttct gccaattgct ttatttctcc atcaagttct tttcctaaat tatacatatt
    20341 atgttgtaaa ctattattgt aataccacat aaatccagaa attaaaaatg caattatact
    20401 tatgtaacct aatacttttt taaaccccat tacaattgat ttatgcctat ccaatgataa
    20461 agatagttct tttattatat tacataattc atactgcatt tcatcaccat catcaaatct
    20521 ttcaattgct tttttacatg cttcttcttc atctaatcct tctaatttaa attcatttgc
    20581 agattctata agatggcatc tcatttcctg ttttaattct atagtacttt tattgtcaca
    20641 ttttttatat agtttatcta aatattcatc tattagtttc ataaattaat ccacctctcc
    20701 caaaaattga ttcataacct ttttaataaa tctccattct tgtttcttta tttctaaaaa
    20761 atctttacct tcattagtta aattataata ttttcttctt ccacctgaac tttgttcatt
    20821 gctataataa gatttaatta aatttttaga ttccattctt ttaagtgcaa gatacattgt
    20881 tccttctttt aattcaaact cacttctttc tcttacttgc tttgcaattt catatccata
    20941 acaatctttc ttttctaata tagaaacaat taatatatcg atataacctt ttaacacttc
    21001 tttatttaac tgcattatgt accaccgcct tttcacctac tactctataa tgtatagtac
    21061 ttatatttac tataataata catagtactc tat
//
LOCUS       DAMKMI010000061        20762 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMKMI010000061 DAMKMI010000000
VERSION     DAMKMI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.062"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="PC429_002046"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="PC429_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF3847551.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="PC429_002047"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="PC429_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF3847552.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="PC429_002048"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="PC429_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF3847553.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="PC429_002049"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="PC429_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF3847554.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="PC429_002050"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="PC429_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF3847555.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="PC429_002051"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="PC429_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF3847556.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="PC429_002052"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="PC429_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF3847557.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="PC429_002053"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="PC429_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF3847558.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="PC429_002054"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="PC429_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF3847559.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="PC429_002055"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="PC429_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF3847560.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC429_002056"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC429_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF3847561.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="PC429_002057"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="PC429_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF3847562.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="PC429_002058"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="PC429_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF3847563.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="PC429_002059"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="PC429_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF3847564.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="PC429_002060"
     CDS             6989..7174
                     /locus_tag="PC429_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF3847565.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="PC429_002061"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="PC429_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF3847566.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="PC429_002062"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="PC429_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF3847567.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="PC429_002063"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="PC429_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF3847568.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="PC429_002064"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="PC429_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF3847569.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="PC429_002065"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="PC429_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF3847570.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="PC429_002066"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="PC429_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF3847571.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="PC429_002067"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="PC429_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF3847572.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="PC429_002068"
     CDS             11117..11767
                     /locus_tag="PC429_002068"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF3847573.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="PC429_002069"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="PC429_002069"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF3847574.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="PC429_002070"
     CDS             12536..12805
                     /locus_tag="PC429_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF3847575.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="PC429_002071"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="PC429_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF3847576.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC429_002072"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC429_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF3847577.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="PC429_002073"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="PC429_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF3847578.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="PC429_002074"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="PC429_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF3847579.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="PC429_002075"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="PC429_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF3847580.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="PC429_002076"
     CDS             14901..15848
                     /locus_tag="PC429_002076"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF3847581.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="PC429_002077"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="PC429_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF3847582.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="PC429_002078"
     CDS             16330..17163
                     /locus_tag="PC429_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF3847583.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="PC429_002079"
     CDS             17151..18017
                     /locus_tag="PC429_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF3847584.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="PC429_002080"
     CDS             18011..18814
                     /locus_tag="PC429_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF3847585.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="PC429_002081"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="PC429_002081"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF3847586.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="PC429_002082"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="PC429_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF3847587.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="PC429_002083"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="PC429_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF3847588.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
BASE COUNT         8292 a         2448 c         4324 g         5698 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaagaggggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataaa atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaaggag ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaatgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgaaaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctacttcat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagctt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaattaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgag
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtgga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtattaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtgttta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagaacttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tacagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcctt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaga tttaaatttt atttttataa
    20761 ta
//
LOCUS       DAMKMI010000062        20756 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMKMI010000062 DAMKMI010000000
VERSION     DAMKMI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.063"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            101..1132
                     /locus_tag="PC429_002084"
     CDS             101..1132
                     /locus_tag="PC429_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF3847589.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1265..2563
                     /locus_tag="PC429_002085"
     CDS             1265..2563
                     /locus_tag="PC429_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF3847590.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(2677..2922)
                     /locus_tag="PC429_002086"
     CDS             complement(2677..2922)
                     /locus_tag="PC429_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF3847591.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3070..3936
                     /locus_tag="PC429_002087"
     CDS             3070..3936
                     /locus_tag="PC429_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF3847592.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(3940..4476)
                     /locus_tag="PC429_002088"
     CDS             complement(3940..4476)
                     /locus_tag="PC429_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF3847593.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(4667..4870)
                     /locus_tag="PC429_002089"
     CDS             complement(4667..4870)
                     /locus_tag="PC429_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF3847594.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5161..5925
                     /locus_tag="PC429_002090"
     CDS             5161..5925
                     /locus_tag="PC429_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3847595.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6314..7627
                     /gene="prdC"
                     /locus_tag="PC429_002091"
     CDS             6314..7627
                     /gene="prdC"
                     /locus_tag="PC429_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF3847596.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8031..8116
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            8698..10947
                     /locus_tag="PC429_002092"
     CDS             8698..10947
                     /locus_tag="PC429_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF3847597.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
     gene            11354..13114
                     /gene="prdR"
                     /locus_tag="PC429_002093"
     CDS             11354..13114
                     /gene="prdR"
                     /locus_tag="PC429_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF3847598.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
     gene            13542..15422
                     /gene="prdA"
                     /locus_tag="PC429_002094"
     CDS             13542..15422
                     /gene="prdA"
                     /locus_tag="PC429_002094"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF3847599.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            15503..15787
                     /locus_tag="PC429_002095"
     CDS             15503..15787
                     /locus_tag="PC429_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847600.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            15825..16550
                     /gene="prdB"
                     /locus_tag="PC429_002096"
     CDS             15825..16550
                     /gene="prdB"
                     /locus_tag="PC429_002096"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:16275..16277,aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF3847601.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            16630..17388
                     /gene="prdD"
                     /locus_tag="PC429_002097"
     CDS             16630..17388
                     /gene="prdD"
                     /locus_tag="PC429_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF3847602.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            17403..17870
                     /locus_tag="PC429_002098"
     CDS             17403..17870
                     /locus_tag="PC429_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF3847603.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            17888..18370
                     /locus_tag="PC429_002099"
                     /pseudo
     CDS             17888..18370
                     /locus_tag="PC429_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
     gene            18390..19397
                     /locus_tag="PC429_002100"
     CDS             18390..19397
                     /locus_tag="PC429_002100"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF3847604.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            19517..20398
                     /locus_tag="PC429_002101"
     CDS             19517..20398
                     /locus_tag="PC429_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF3847605.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
BASE COUNT         8286 a         2415 c         3847 g         6208 t
ORIGIN      
        1 atgtttttag caagaaatat agcatttaaa agataaattg tgcaacttaa atcaaagttt
       61 aagatgaact attaacatag atatattaaa aacttttgta atggagaata tatgtgatat
      121 gaataataaa gttaaggttg cagtattaga tacaggaata gacaaagaac atgattactt
      181 aaaagataat ttagttggag gaattgcttt tgaatgtatg catgattata tctttatatc
      241 agataaattt gatgatgaag atggtcatgg aactgcttgt gcatcaataa taaagaaaga
      301 gtatgaagat gtagagttat ttgtaataaa aattttgaga aaagatggta ttacaaatat
      361 aaaagttctt gaagaagcat taagatacct tttagacact aacataagat taattaattt
      421 aagtttatca gtcataggag tagaaagtgt taagggctta tttgaagttt gttatgaatt
      481 gtttagaaaa ggaaaaataa tagtatgttc actcgctaat gattttgact taagttatcc
      541 agctatgttt aacaatgtta taggtgtaag aggatttact ttagatggag atgattcatt
      601 ttggtacaac aaaaaatatg atgttcaatg tgttatggat agcaattcat atataagttg
      661 tgatataaat aattcctata gattaccacc aaaatgtaat agttatttat cagcaaagct
      721 tactggtaaa atagctaaga tattatcaga aaaaccaggc ataacaatat ctgatttaaa
      781 tgataagctt gaatcattag caactagaaa tcattgggat agttgtgatt ttgatgagtg
      841 tagtagaatt gtaaacttta aattggattt gtatgacaaa gaaaatgctc tttttatgaa
      901 agttgctgac gttgttaggg attgcttaga tattgaagag aataatgaaa aactctttaa
      961 gggtagcctt tttaataagg agataggctt ggtttatgat aattgtttta atctgttgaa
     1021 aaagctagag catagatttg acataaagtt taattatatg gacatctcaa aatatgactt
     1081 agtttcaata tatacactga ctgaattagt ggaaagatat aaaaacttat aaataaagat
     1141 ttgtagctaa atttaatgta gaaggtatta aattatatgt attctttgtc ggtagattaa
     1201 aagtaaaaaa tatactataa ttagatagca taagagaaaa tgccaataca gggggagtta
     1261 gataatgaaa tatgaagaag cattagagta catatcacaa actaataaat tcggtataag
     1321 attaggatta gaaaatatcg gaaagttatt agaattatta ggaaatccac aagaaacttt
     1381 gaatataata catgtagctg gaactaatgg aaaaggttct gtgtgttctt ttgtttctaa
     1441 tatactaaga gagtgtggat ataaagttgg tttatataca tctccatatt tagaaacttt
     1501 tactgagcgt ataagagtaa atggacaaaa tataccacag gatgatgttg ctagaataat
     1561 tgagttaata aaagaaaaaa tagagattat ggtaaaagaa ggctatgcat atccaacaga
     1621 atttgaagtg gttactgcaa tggcatttta ttactattca gaacaaaagg tagactttgt
     1681 ggcattagaa gttggtttag gtggcagata tgatgctaca aatataataa caaaatcact
     1741 tgtaagtgtg atagtatcta taagtttgga ccatacaggt atacttggag atacaataga
     1801 aaaaatagcg tacgaaaagg ctggaataat aaaggaaaat ggagtagtac tagtatatga
     1861 ccagactgat gaagcaaagg atgttataaa gtcagtttgt aaagagaaaa aagctaaata
     1921 tatagaagtt gattttgatg atataaacat aaaaaaatca gatataaatt ctcaaatata
     1981 cgattgtact gtaatgaaag aaacttatag agaccttgaa ataaaattga taggcgaaca
     2041 tcaaataaac aattctatct tggcaattag tgtaataaaa tatctaaaag acataaataa
     2101 attagcaaat ataaatgaag aatctataag aaaaggactt ataaatacta aatggccagg
     2161 aagaatagaa aagataaaag aaaatccaat ttttataata gatggtgcac ataatgaaga
     2221 tggagcaaaa tctttggcaa aagcacttga taagaatttt aaaggtagaa aattaacctt
     2281 attaataggt atgttagaag ataaagatat tgatagtgtt ttagagatat tgttgccaca
     2341 ttttaataag gtaataacaa caacaccaag taacccaaga gccataaatt cagatatatt
     2401 aagagaaaaa gtattaaaat atgtagatga tgtaacatca aagcatgaga tagaggatgc
     2461 agtaaattat acattagaaa catcaagtga ggatgatatt ataataagtg caggttcatt
     2521 atatatgatt ggtacagtga gaactttagt aaagaaatta taattttata aaataagtat
     2581 acaaataaaa atataaaaaa gagttgtcta aaaaatggac aactcttttt atataaaata
     2641 agtttttaat aattttactg atgtttatat aagtaattat atagattgac gtatagcttt
     2701 agataattgg tctgggcaag aagtaccttt tccaccacag tcaataccat ttaatttatc
     2761 agcaacttca agagcatttt ggccaattat taagcttcta agtcctgata aattaccatt
     2821 acatcctcct ataaattcag catcaacaat cacattattt tcatcaactt caaaaatcat
     2881 ttctctacaa catacaccac ttggattaaa tgttactttc attaaaaaga cctccattaa
     2941 atattatttc tttttttaac attaataata ataccaaata tatgcgtaat aatgcaacta
     3001 taaagcataa tacaagctaa tgaaataata tattattgta gtatatgtat tgtagagagg
     3061 aggggttata tgaaaatcta tatttctaac tacttatcaa agagtattag catagttgat
     3121 tattctacac ttgaactaga aaaagaaata gtattagaag ataatatata tccacaccat
     3181 ttttgcatag aaaaagaaaa aaatttaata tacataccta gttcaagtaa tgggatactc
     3241 tatgtcttag atttaagtaa tgataaaata attgatactg tctctatagg aggaagtctt
     3301 agtcaagttg tacttagcga taatgaattg tttgtggcaa atgaagattc taatagtata
     3361 tatgtactag ataaaaacac attaaatcca ataggaatta ttggagttga taatatgcca
     3421 catggatttg actttgatag agaaagcaaa aagttatatg taccgtgtat aaactcgata
     3481 gtatgtatag atacaattaa taaaaatata caaaagaaaa taaatatgga ctttagagct
     3541 tggcatatag aacttgataa acaaaagaaa gaaatgtata tatcaacact tgatggaaag
     3601 cttataataa tagatgaagt aagcatggat ataaaaaagg tattatatga atttttatta
     3661 ccagtagaaa taagatttaa ttatagtggc aaaaaagttt atgttgctga tttgggttat
     3721 aataatgtaa gaattttaga ttatacaacg agtagataca ttggaaatat tgaaataaat
     3781 gggattcccc aaggattaga aatatctaaa gatgaaaagt tactatttgt atcagataca
     3841 caagaaaatt cagtaaaagt ttatgaaact gcaaataaca aattaataaa agaaatcaag
     3901 gtaggaaaag agcctacaac tataatttgt ttgtaggctt taatgtatgt ctcttataag
     3961 taaatcgata acttcggcat acatataaga tgcattattt ttccagttat tgcaaataag
     4021 ttttgcttgt ttctctgatg aaacatttag atttaaatct attaagttag actgattttc
     4081 tatgactcta aggttgacaa taaattcatt atcactttgt tttacaaaac tagactttac
     4141 ttgagtatct gctagtagat tttctttgtt agaatctata tacttgtcta ttttttcagt
     4201 aacactagta ggtattcgat taaagaagta ttctagactt tctacgcctc tttgagtaag
     4261 agcataatat tctctgtcag agtctttata tgtagtcaaa aacttagatt ccataagctg
     4321 agataaaagc tgttgtaatg aaaaataatt catcatctca gtctctaata ctacttgagt
     4381 tatttgagaa ttagttaaat ccattttaat tttttctaat atatataaaa ttaaaagttt
     4441 gtgataggct aattcttcag acgagttttc aaacataaat atcatcctcc gtcaaactat
     4501 ttctatatat aatatattaa actaatttgt acaaaattaa aagaacaatt tgtattgtat
     4561 tattaatctt taataattca tataaaaaca attttataaa taaatataga aaaacagggc
     4621 tgctaattag cagccctgtt gatacccagg tgtcaaaatt tactatttat tttccagcca
     4681 tttgtctttc agccatttca actaatttct tagtcatata tccaccaaca tatccatttt
     4741 ctctagctgt taagttacct ttgtctatgt tttcatagtt agataaacct atttcgttag
     4801 ctatttctaa tttcatttgg tttaaagcag cttttgcttc tggaactact gttctgtttg
     4861 aatttgacat agtaaatttc ctcctcaaat aattagttta tgatgtctaa caacactttt
     4921 atttatgtga tgttaatact aatttctcca aaataaaaat aatatataca gaaaaataaa
     4981 ggtaaaaatg taaaaaatat aaaaaaatta aataaatgag tattttagag agtttaatta
     5041 ggtcaatata ggttttattt ttttagttat tgtcttataa attaaatatt acagtacttt
     5101 tttgagctga aaaaaaataa attttaaata tagaagaaat tatttcaaag ggggaatagt
     5161 atgaaaagag aagttaaaag gactaaagct ttgatatata gtgttttatt tttttcatta
     5221 ttattagtgt taatatttgt ttttgtttat ggaataaaat acatatgttc taaatctgac
     5281 aattcaaaaa tgcctatata taaagttgat acaaatgaga aaaaaatagc tttgagtttt
     5341 gatgtagcat ggggaactga taatatggat gatattttaa aaattttaga taaacataat
     5401 gtaaaagcga ccttcttttt agttggtagt tgggtggatg ataatgaaga aattgtaaaa
     5461 gctatagatg aaaaaggaca tgaaattgga aatcattcaa atactcatgc caatttaaag
     5521 gaaatatcaa aagaagacat aacaaaagag atagaaacta catcagaaaa aatatataat
     5581 ataacaggaa aaaggactaa tttatttaga cctccttttg gggacgtaaa caataaagct
     5641 atggacattt gtagtgattt aggttataaa gtaattaagt gggatgtaga ttctatagac
     5701 tggaaggagc ttggaccaaa tcatgtaatt gaaaaagtaa taaaagaatc tcaacctggt
     5761 tcaatagttt tattccatgc taatataaat gatgtagata attatttgga tactataata
     5821 agtcgactaa aaaaagatgg atacagtcta gtcaaaatat ctgatttgtt gtataaagat
     5881 aattatgtag ttgattccaa tggagtgcaa aaaactaaga attaataagt aataaatgta
     5941 tattttaagt aaagttgtga taaattatat agtatatgtt agaaaagtgt cgataaaaag
     6001 acggtgtcta ttttttgaca ctttttatac atgtatgcaa tatcaataat tatagattaa
     6061 attgattaaa aaagtgtcta ataattgact tttttttgat gggtttaaaa atttagaaga
     6121 attaatgaat gctgtaagac agtatttcca aagggtataa gtttgtttaa aagttggcat
     6181 agaaattgct ttgttaataa gcgatatgtt ctatagaaat aaacaatatg ttaagacctt
     6241 ataatagaaa aatagcatta acattatttg aacagcttat aaaaaaatta atatacttaa
     6301 gggggcgtac tagttgaaca aactatattc tattttatta aaacaacacg ttggtggtcc
     6361 agataagcca gtagtaagtg ttggagatgt agtaaaaaaa ggaactctaa tcgcagaacc
     6421 aactggatta ggggcaaata tttatgccag tgttagtgga aaaataagtg aaataaatga
     6481 ccaagcgata gtaattgaag ctgatgaagt acaagaagat acttttgaac cattaaaagg
     6541 agaaggtata cttgacttaa ttaaagaagc tggagtagta ggaatgggtg gagcaggatt
     6601 ccctactcat gtaaaattaa acatagattt aaatggtgga acaatattag caaatgcagc
     6661 tgaatgtgaa cctttattag ctcataatat aaaagaaata gaagaaagac cagaaatagt
     6721 ttatcaaggt ataaaatatg ctatggaagt tactaatgct ggaaaaggta tgctagcaat
     6781 aaaaagtaag catccaaaag caatagaagc tttcaaaaaa gtaataaagc caggagacaa
     6841 catagaagtt gcagaactag ttgatatgta cccaatggga gaagagagag caatagtaag
     6901 agatgttcta ggaaaattac ttgagccaac tcaattacct tcagaggcta atgcagtagt
     6961 aataaacgta gaaacactaa ctagaatagt agaggctgtt gagcaaaaaa gaccagttat
     7021 atctaaaaat ataactgtag ttggtcaatt aaacagtgga aaagaatcta tagtgtttga
     7081 agatgtgcca attggaacaa ctgtcggaga attaattgaa agagctggtg gaataaaagg
     7141 tgaatatggt gaaataatcc taggaggacc tttcactggt aaagctacta ctttagatgc
     7201 tccaataact aaaactagtg gtggtataat agttactatg ccatttgtta atgaaaagag
     7261 aaaaatgggt cttttagttt gtgcatgtgg acctaatgaa gaaagaatga gagatatagc
     7321 aactaaaatg ggagttactg atatagtatc agttcaaaaa tgtaaacaag ctcaagaaat
     7381 taaaggtgca ttaaaatgtg aaaatccagg acactgtcca ggacaagctc aaaaatgtat
     7441 tgaatttaag aaagctggag cagaagttat attaatagga aactgtactg actgtagtaa
     7501 cacagtaatg ggttctgcac caaaattaaa attaggtgta tatcatatta ctgaccatgt
     7561 aatgagaaca gttaatcacc cattaataag aagaataaaa ggtgacgtaa gattaaaaat
     7621 aaaataatag ataaatagaa gttagacatc tttagaagta aaacttaaaa gttacttcta
     7681 aggatgtttt ttaatgtatt ttgagggcaa tatatatatt gtaaaataaa tggaaaaaat
     7741 agacaaaata tgctagatat tgtataatga taacaatagc tattacaatt aatattggac
     7801 aaaaataatg atactcaagt atgtggtttg tattaatatg catattatat taagtcaact
     7861 atccgataga gtaaaaggtt tttgtgatat ttgaagatac tctatagtat cttttttatt
     7921 ataaaaacta atttttgtct tttattagat aagtatctag gatactaatt tatattttat
     7981 tgagttgtta aaaaagtgca cttaaaaagt taaaaaagaa aaattaaaaa cctatttata
     8041 gaaactgtga agtatatctt aaacctgggc acttaaaaga tatatggagt tagtagtgca
     8101 acctgctata aatataaata ggagtaactt ttaattgtca aaaatatata aaataaatac
     8161 ataaataagt aattaataga agatagatat aaatatattt ttgaaatgct ccctgcattc
     8221 ataacgagtg tagagtggca gatatctgcc caaaactcct ttaattgctg gaagttccta
     8281 aagctaacta aaccacaaca taatcatgaa ataaggataa gtgtgataga gtgtgcattt
     8341 aataagcact tgaggttacg aaagtagaaa aaattagtta gatagcataa ggttaaatcc
     8401 taagtgcctt gaaaatggat aatcagcagg taagcttcga aaagaagaaa cttcaacgac
     8461 tagagagaaa tctcgtacct acaagtgtgg ggaaatgggg agtacctaat ttatggttag
     8521 gtaatgatat agtctgtgct taatagaaat attaagaagt tcataggaga actggttagg
     8581 agtaacgacc ctaattgaac acactaaaga tgtattattg aataagattc atataatatt
     8641 taataatttt aattatatta agtgaataca atatatgttt ttagtatcag gagaattttg
     8701 aaaacaaaaa ttaaaaaatc aagtataatc agtttagtaa ttgcttttag tatggtattt
     8761 actgcattta caccaatagt atcttatgct gacgaagtga ctagcaatga tacaatactt
     8821 aatggagaag aacaatcaaa tggtcagaca tcagatgtag agaaaccatc agacggtcag
     8881 gtaccagatg gggagaaacc atcagacagt caagtaccag atgtagagaa accatcagac
     8941 ggtcaggtac cagatgggga gaaaccatca gacagtcaag taccagatgg ggagaaacca
     9001 tcagacggtc aggtaccaga tggggagaaa ccatcagatg gtcagatacc agatggggag
     9061 aaaccatcag atggtcagat accagatgga gagaaaccat cagatggtca gataccagat
     9121 ggggagaaac catcagatgg tcagatgcca gatggagaga agacatcaga tagtcagacg
     9181 ccagatgtag agaaaccatc agatggtcag atgccagatg gggagaaacc attagatgag
     9241 caaactccag aagaagaaaa accattggaa gaagaaatag ttatagagga aatgagttta
     9301 aaacaagata ttgataaaat tcttgatatg actttatctc aaataaataa aatagtatat
     9361 aacttttggg aagatgaaga agatgtaaag gcagatgaac aatcagaaat taaccaagtt
     9421 tttacttcag aagactcatt tattagctta tggtatgaca aaaaagcaaa agtaaaaaat
     9481 tcttgtttac taaaagaaat agatggttca gatagacctt atcatgattt aagatttgat
     9541 gatattacaa agactttaac ttttgactat gtaataaagg gtcttgtagg agcaagctca
     9601 aaagacgaca aatatgtaat agaaggtgaa gatggagagc aaactgcatt ttgttacaat
     9661 aatcatttaa gaccaccaag tagtaatgga aagagtccat atttaccagc agaagatttt
     9721 aatggtcaag aaaaccaaaa tgaagaagca gttaaatcta tactatatgc aggttctgaa
     9781 tttgatggat ttggatataa gcaacaattt aatttaggtg gagaagaaaa tgaattgatg
     9841 acttactcag ctactcaaag tgcaatttgg attattcttg gacaaatgga tgaacaagaa
     9901 agacttaagc agtatcaagg ttctataaac ctttgtgata aacttataga aagagcaaaa
     9961 actgaagagg aaagagctga gtatcagaag aagaaagaag tagcgataaa tataaaagct
    10021 tatttagaag cattgttaaa agcaggtcgt gaagagttaa agccaaataa tacaggaaaa
    10081 cctagtttga gtaatggttt aactaagatt aactttgaaa aaaatgaaga tggtacttat
    10141 gaaacagaag ctgtagcctt agtaggttat agtggtgtag taaagttaaa actaccagat
    10201 ggagtaactg cttatgatgg agatggtaat ataataggta caggcgaagt agaaatttca
    10261 acgcaacaga aatttaaact taagagtgtt ggaaaaccag atgccaaagc taatatttct
    10321 gctgtatctt atgattatat ttttccaaaa gctattcaat attataaggc agtgcttgat
    10381 ttaggacaaa aagaccattc atcttcatta ccatctagca agcaaaattt attaagttat
    10441 acaatagaga aaaagaatgg tcaagaagtt aactttaata taggtttacc aacagatgat
    10501 gatgatgttg taaatccacc agtgccacca atagatgatg atgttgtaaa tccaccggtg
    10561 ccaccaacag atgatacaaa tggacataag ccaaaaccat caccaccaat agatgataca
    10621 gtaataaatc caccagtacc accaatggat gatacaataa taaatccacc agtaccacca
    10681 acggaggatg cagtgttaaa tccaccagtg ccaccaatgg aggatgcagt gctaaatcca
    10741 ccagtgccac caacagagga tacagtgtta aatccaccag taccatcagt aagtgataca
    10801 gtggaaaaaa ctccagaact atcaagagat gatacaataa taaaatcacc aaaaacaggt
    10861 gatgaaactc agctaatgtc atatgtattt attagtgtca ttgcaatttg tggattagcg
    10921 tatcaatgta aaataaaaag aaattaatct agattattat taagtttaaa ataaaaagtc
    10981 tcaaattgat ttataagtca ttttggggct tttttatgta aatataatat gcaaatatag
    11041 tttgtttaaa aatatctatt ttaattatat tcttaaattt attattggat acaggcattc
    11101 attaattgtt tgatgaatag aaaaatgttt tgaaggtgaa aatagtattg aaaaaataat
    11161 atactaaggg tataattcat ttgatttcat ttgtataaat ttaattataa attttaaagt
    11221 gattatataa atttaaatgc taattactta ataaatttta ataataatta tttaataaat
    11281 aattattata atatgctaga ataaatatat ataaataagt tttgatgagg tactactata
    11341 aaggggggac tttatgtttt cgataccaga agtaaaaaaa gtagaagagg taatggatac
    11401 aaaatttaca actatagacg aagacacaag aattgaagat gcaataaaag aaatgataaa
    11461 aagcaacaca aaaactctaa tggtaataga ttcatctgac caattaaaag gcattatatc
    11521 catgacggat atacataatc tttatgagat gcataaaaag tatgaaggtc aaccagtaaa
    11581 acttataatg aagaaggatg taatatatgt caacgaaggt ttaaccttag atgaatgtag
    11641 agatataatg atattaaaaa atatagggat tcttcctgtt cttagagata ataaaattat
    11701 tggagttcta aaacaagagc acattaggga ctatctatat atgcatttag aagattatgg
    11761 attaactttg aagtatatta taggtcaaat taaagaagga atttgtgcta taaataacga
    11821 aggtgttgtt atcttgtgga ataagtttat ggaggaaagg tatgatataa agtctgaaga
    11881 tatagttggt cgaccgatga atgaattttt ggaaaatact atttcagaaa aagtactaaa
    11941 tagtaaagtt ggaatgagtg acttgtattt tacagataaa aaagaaaata tgtatgcatt
    12001 agttcatgca aatcctatat tttataaaga agaatttatc ggtgttgtat gtacagaagt
    12061 tgatgttaca gaagctaaga tattagctct tgaacttgaa aaagtaaatg atacattaaa
    12121 atatctaaag aacgaagtca aaaatttatc taaaggtagt tttgataaga ttttaggtaa
    12181 aagttataaa ttggaaaaat cgaaagcgat tgcaaaacaa gtggctagaa caaatagtag
    12241 tatatttatt tggggcgaaa gtggtacagg taaagaagta tttgcaagag ctattcatga
    12301 ttatagtgag agaaaagggc aatttatacc tgttaactgt agtgccatac caaatgagtt
    12361 atttgaaagt gagttttttg ggtatgaatc aggtgcattt acaggagcaa gtaaaaaggg
    12421 tcgaataggt atatttgaac ttgcaaaaga tggaactgta tttttagatg aaattgccga
    12481 tttacctctt agtatgcaag caaagttgct tagagtatta caggaaaaag aaataagaag
    12541 agttggtgga gataccacaa taaaaataaa tcctagaata atatcagcta ccaataaaga
    12601 tttgagtaaa atggtaaagg cagaaaaatt tagagaagat ttatactata gattaaatgt
    12661 tgttgaaata aagattcctc cacttagaga gagaaaagaa gacataggat tattggttca
    12721 tagcttttta gaggagatat gtaaacaaaa taataagcct gttcttacaa ttagcaaaga
    12781 tgtaatagat atatttcaga attatagatg gaaaggtaat attagagaat taaaaaatac
    12841 gattgaaaat attgttgtat tgtcacaaaa ttctaaaata gatgtggatg atgtaccaag
    12901 ttatatgatg gattctacaa ataatagtac tgaagaagaa gagtatcctt tagatttaac
    12961 gaaggcaact caaaaaattg aaataaagaa tataaccaag gcacttaaaa tgtctaatgg
    13021 aaataaagct aaagcagcta agatattaaa tatacccaga acaactttgt attataagat
    13081 agaccaatac aaaatagatg tgtctaaaat atgacacatg actaaatttt gacaaaatga
    13141 taaactttaa tttaaaacct agtaaagcca acacttgtat aagtgttgtg tatatagatg
    13201 tgttaaaaaa aatgaaaaag tgttgattaa ttgacacttt tttgtttgag aaaatgttta
    13261 caaatacaaa aaataacata ttttaaataa atatttatat aatatttggc taaaaatagc
    13321 gatagatgaa gaatctatta tagaaaaaat ctttttgtta ataagttggc ataggaattg
    13381 cttataatat aaagtgactt taagtgagaa aaaaataata ttaatcataa caaattatgt
    13441 aggttttaat ttacttaatt tctcagttaa ggggttacct gttgaggaat tgagatttaa
    13501 aataataaat aagaaaataa tgtaaggagg tattttatac tatgtcaata actttagaaa
    13561 cagctcaagc ccatgcaaat gacccagcag tttgttgttg tagatttgaa gcgggaacaa
    13621 ttatagcgcc agaaaactta gaagatccag caatatttgc agacttagag gattctggat
    13681 tattaacaat accagaaaat ggattaacta taggtcaagt actaggagct aagttaaaag
    13741 aaactttaga tgcactttct ccaatgacta cagataacgt agaaggatac aaagcaggag
    13801 aggctaaaga agaagtagta gaagaaacag tagaagaagc agctccagta tcagaagcag
    13861 cagtagttcc agtaagcaca ggagttgcag gtgaaacagt taaaatacac ataggtgaag
    13921 gtaagaacat aagcttagag atacctttat cagtagctgg tcaagcagga gttgctgctc
    13981 cagtagcaaa cgttgctgct ccagtggcaa gtgcagcagc agaagtagct ccaaaagttg
    14041 aagaaaagaa acttttaaga agcttaacta aaaaacactt taaaatagat aaagttgaat
    14101 ttgctgatga aactaaaata gaaggaacta ctttatacat cagaaacgca gaagaaatat
    14161 gtaaagaagc taatgaaact caagagttag ttgtagatat gaagttagaa ataataactc
    14221 ctgataaata tgaaacttac agtgaagctg tattagatat acaaccaatc gctactaaag
    14281 aagaaggcga attaggttca ggtataacta gagttataga tggagctgta atggtattaa
    14341 ctggtacaga tgaagatgga gttcaaatag gtgaatttgg ttcttcagaa ggtgagttaa
    14401 atactactat aatgtggggt agaccaggtg ctgctgacaa aggtgaaata ttcatcaaag
    14461 gtcaagtaac aataaaagca ggaactaaca tggaaagacc aggaccttta gctgctcacc
    14521 gtgcatttga ctatgtaact caagaaataa gagaagcatt aaagaaagtt gacaactctt
    14581 tagtagttga tgaagaagta atagagcaat acagaagaga aggtaaaaag aaagttgttg
    14641 ttataaaaga aataatggga caaggtgcaa tgcatgataa cctaatatta ccagttgagc
    14701 cagttggtac attaggagct caaccaaacg ttgacttagg aaacatgcca gttgtattat
    14761 ctccacttga agtattagat ggtggtatcc atgcattaac ttgtatagga cctgcatcaa
    14821 aagaaatgtc aagacattac tggagagagc cattagtaat aagagctatg gaagacgaag
    14881 aaatagattt agtaggtgtt gtatttgttg gttctccaca agtaaatgct gagaaattct
    14941 atgtatctaa gagattaggt atgttagttg aagctatgga agttgatgga gctgtagtaa
    15001 ctactgaagg tttcggaaac aaccatatag atttcgcatc tcacatagag caaataggta
    15061 tgagaggtat accagtagtt ggtgtaagtt tctcagctgt tcaaggtgct ctagttgttg
    15121 gtaataaata catgactcac atggtagaca acaataagtc taagcaaggt atagagaatg
    15181 aaatattatc taacaacact ttagctccag aagatgctgt tagaataatg gctatgctta
    15241 aaaatgctat agaaggtgta gaagttaaag ctcctgaaag aaaatggaat ccaaatgtta
    15301 aattaaataa catagaagct atagaaaaag ttacaggaga aaaaatagta ttagaagaga
    15361 atgagcaatc tctaccaatg agtaagaaga gaagagaaat atacgaaaaa gacgaaaact
    15421 aaaatttaga tactaactaa gatttagaca ataacataaa tttagatcca agtttaaaac
    15481 agtatagaga ggtagaatta taatggatag gttaaaatat atctcatcag agacttttta
    15541 tgaaggtatt atagttgaca tcaaaggcgg tggagtgact atagacctaa aaggtagact
    15601 tggacaattt aaaataccta atagaatgct tataactgac tacgaactta agataggtca
    15661 agaagttgga ttcatgctaa gctatcctga ggtattaagt ccagagccta atcaagagta
    15721 tgtagaaaat attaggagag aaaaagaaaa acaagcagaa atgcaatcaa aacaagaaga
    15781 aaaataataa ataatcgttt gaaaatagaa gggagaggaa atatatgagc cttacaacag
    15841 tacaaggact tcaatctgaa atattcgttc caataacacc tcctccagtt tggactcctg
    15901 taactaaaga attaaaagat atgactatag ctttagctac agcagctggt gttcatttaa
    15961 aatctgacaa gagatttaat ctagcaggag atactacatt tagagctata cctaatacag
    16021 ctacagttga tgaaatgatg gtatcacatg gtggatatga caatggagat gtaaacaaag
    16081 atataaactg tatgttccct atagatagat tacatgaatt agctgcagaa ggatttatca
    16141 aaggagtagc tcctatgcac tatgcattca tgggtggtgg aggaaaccaa catgtcttca
    16201 ctgaagaaac tggtcctgct atcgctgcta aacttaagga agagggagta gacggtgtag
    16261 ttatgacagc tggctgaggt acttgccata gaactgccgt gatcgtgcag agagcaatag
    16321 aggaagctgg aatacctaca ataataatag cagctcttcc tccagtagtt agacaaaacg
    16381 gaactcctag agcagttgct ccattagttc caatgggtgc taatgctggt gaaccacata
    16441 atatagaaat gcaaacacat atattaagag atacattaga gcaattagtt gcaataccat
    16501 ctgctggtaa gatagttcca ttaccatacg aatataaagc tcacgtttaa aatgtaattt
    16561 atatttgaaa atatagaacc cttctcctac acggttagga gaggggtttt aagaacaaag
    16621 gtggatatta tggaagaaaa aataatgaga agacttgtta taaaaccgtt tcatatgaat
    16681 gaagttaatt tcggttcaaa aacttctata aagaaagatg tacttacaat agatttatca
    16741 agtatagatg aaataaaaga aagagaagag ttaataacag acataaaagt agacattata
    16801 aaacctggag attatgacag agaaattaat actattatgg acattatacc aatatcaaca
    16861 aaagttttag gcagactggg agagggtatt acacatactt taacgggtgt atatgtaatg
    16921 ttaactggtg cagatgaaga tggaaatcaa atgcatgaat ttggttcttc agaaggaatt
    16981 ttgaaagacc aaatggtatt tgggagatat ggaactccat cagttgaaga ttatataatc
    17041 catgtagatg taactttaaa gggaggttta ccttttgaaa gaacacttcc acttgcagca
    17101 ttcagagcat gtgatgattt tatacaagaa ataagagcgt ctttgaaaat ggaagacgga
    17161 agaaatgcta ctcaagttcg tgaatatttt gacaaaattc gaccaaatgc aaaaaaggtt
    17221 gtcatagtaa aacaaatagc aggtcaaggt gctatgtatg acaatcagtt attctcaaag
    17281 gaacctagtg gatttgaagg aggaacatca attatagata tgggaaatgt acctatgatt
    17341 atatctccta atgaatacag agatggtgcc cttagagcta tgacttaaga gaggtggata
    17401 ttatgggaat aggaccttca acaaaagaaa catctcttca tcactttaga gacccacttt
    17461 tagacatagt tgaaagtgat aaggatgtag atttacttgg agtaatagta gtaggaactc
    17521 cagatggaaa tgaaaataaa acctttgtgg gacaaagaac ggccgcttgg ctggaagcca
    17581 tgagagtaga tggagctata gtttcatcag atggttgggg aaactcacat gttgactacg
    17641 ctaacacatt tgaggaaata ggaaaaagag atatacctgt agttggagtt acatttaatg
    17701 ggactcaagc taagtttgtt gtaagcaatc aatatatgga taccatagtt gatatgaaca
    17761 aatcaaaaga aggtattgag acagaagtag ttggagaaaa caacacaaat gaaatagatg
    17821 ctaaaaaagc tttggcattt ttaaaactta aaatgagaaa acatggataa aaaatttaag
    17881 taagggaatg agttttgtgg gaatttctac atcaatgaaa aacacatcac ttcatatatt
    17941 tagagacccg ttactagatt tgatagataa tgattctgac atagacttgt taggagtggt
    18001 tgtagttggg acatcagagc acaatgaatg gaaaacattt ttagcaacta gacttggaag
    18061 atggattgaa tcattgcgac ctgatggagt gattataact ttagatatgg caggaaacca
    18121 gcacattgat tttacaaatg ctatagcaga gtttgtaaaa tcagatatac ctacagtagg
    18181 tcttactatt atgggagcag atggattagt aattaccaat ccatagttgg ataaagctac
    18241 cataattgat tataaaaaga caacaggcta catagaaaca gaagtagttg gagacaatca
    18301 catggatgaa gttgatgtta aaaaagcatt ggctttccta aaattaaaaa tgcgtaaaga
    18361 cgcaaaataa acctaagggg gaatttgata tgaaatttag cagaagtata caagctatag
    18421 actctcatac agcaggtgaa gcaactagaa tagttgtagg tggaatacct aacataaaag
    18481 gaaattctat gcctgagaaa aaagaatatt tagaggaaaa tctagattat ttaagaactg
    18541 ctataatgtt agagccaaga ggacataatg atatgtttgg ttctgtaatg actcaacctt
    18601 gttgcccaga tgctgacttt ggaataatct tcatggatgg tggcggatac cttaacatgt
    18661 gtggccatgg tacaatagga gctatgacag cagctataga aacaggtgta gttccagcag
    18721 tagagcctgt aactcatgta gttatggaag ctccagcagg aataataaga ggagacgtta
    18781 cagttgtaga tggaaaagct aaagaagtat cattcttaaa cgtaccagct ttcttatata
    18841 aagaaggtgt tgaagttgac ttaccaggtg ttggaactgt taaatttgac atatcatttg
    18901 gtggaagctt ctttgctata atacatgcaa gtcaattagg tttaaaaatc gaacctcaaa
    18961 atgctggtaa attaactgaa ctagctatga aacttagaga tataataaat gagaaaatag
    19021 aaatacaaca tccaacttta gctcatataa aaactgtaga cttagttgaa atatatgatg
    19081 agccaactca tccagaagct acttacaaaa atgtagttat atttggtcaa ggtcaagttg
    19141 acagatctcc atgtggaact ggaacaagtg ctaaattagc tactttacat gctaaaggtg
    19201 aattaaaagt aggagaaaaa ttcgtatatg aaagtatatt aggaacttta ttcaaaggtg
    19261 aaatagttga agaaactaaa gttgcagatt tcaatgctgt agtacctaaa ataactggtt
    19321 ctgcttatat aactggattt aatcattttg taatagatga agaagaccca cttaaacatg
    19381 gatttattct taaataagta ataaataaat taggaaatta aaatagaata gtagccactt
    19441 gtcggttgct attctatagc ttttaggcta taagattcag aatatatgaa aaaataaaaa
    19501 aataggagga tggattatgg tagcaatctt aaaggcatta tatgtagtat tcgcagcaat
    19561 ctttgcagtt gtgtttggta aagacgtagc taaagcaaac aaagagggga aactagaaga
    19621 acaaagtact ccaaaagttg ctgtaacagg atttataaca gacttctttg atacattagg
    19681 aatagggtca tttgctccaa caatagcaat gtcaaaggct ttaaagttaa atataccaga
    19741 taaaaaaatg cctggtacat taaacgtagc acatactatt ccagttgtta cagaagcatt
    19801 catatttaca tcaatcatac cagttgatgg tgttacttta gtatctatgg ttgtggcagc
    19861 agcagttggt tcatatatag gtgcaggaat aatagctaag atggacgaga gaaaaataca
    19921 acttgttatg ggtgtaactt tagcattaac agcaatatta atgttattag gtcatccttg
    19981 gattaacgta ttaccaggtg gaggaaatga attaggtctt actggtgtta aattagtaat
    20041 aggtgtaata ggtaacttta tactaggagc tttaatgaca gctggtatag gtttatatgc
    20101 tccaggtatg gcaatggttt atttcttagg aatgtctgca aaagttgcat tcccaatagt
    20161 tatgggttct tgtgcattat taatgccagt tgcaagtatg aaattcataa aagaagatgc
    20221 atatactaaa aaagcatcag ttataatagc tatatgtggt ttaataggag ttttcgtagc
    20281 tgcatatata gtaaaaactc taccaatgga tatattaaaa gcattagtaa tagtagttat
    20341 agcttatact tctgctacaa tgataatggc tgcaaataaa aacaaaaaga ctgcttagtt
    20401 aaagactact tagtgtttat taaatattac agctaataat aaagattata aactgcttta
    20461 aaatattgaa ttaatatttt agggcagttt tttatttact aaattaatat aaagcaatta
    20521 acatgccaat tatgtaatat atatagtgat ttagtagatt taatgtaaaa aacgttgtat
    20581 ttttaaagaa taaagcataa tttaaagcta aatatttaaa ttttataatc aataagttca
    20641 ataaatgttc atatattaat acagattaat aaaaatataa cctataataa gtctaatata
    20701 acttataatt aagaaaaata agtttataat gactcaatat tcatctgaat tatttg
//
LOCUS       DAMKMI010000063        20361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMKMI010000063 DAMKMI010000000
VERSION     DAMKMI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.064"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            180..734
                     /gene="tcdR"
                     /locus_tag="PC429_002102"
     CDS             180..734
                     /gene="tcdR"
                     /locus_tag="PC429_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF3847606.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            1052..8152
                     /gene="tcdB"
                     /locus_tag="PC429_002103"
                     /gene_synonym="toxB"
     CDS             1052..8152
                     /gene="tcdB"
                     /locus_tag="PC429_002103"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF3847607.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8274..8774
                     /gene="tcdE"
                     /locus_tag="PC429_002104"
     CDS             8274..8774
                     /gene="tcdE"
                     /locus_tag="PC429_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF3847608.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            9103..9285
                     /locus_tag="PC429_002105"
     CDS             9103..9285
                     /locus_tag="PC429_002105"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847609.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9502..17571
                     /gene="tcdA"
                     /locus_tag="PC429_002106"
                     /gene_synonym="toxA"
     CDS             9502..17571
                     /gene="tcdA"
                     /locus_tag="PC429_002106"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF3847610.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNTNTF
                     IASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTHNNNIEGQAILYQNKFL
                     TLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAVTGWQTINGKKYYFNTNTSIAS
                     TGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQNRFLYLH
                     DNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGYKTIDNKNFYFRNGLPQIGVFK
                     GSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIYYFGNNSKAVTGWQTINGKVYY
                     FMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17906..18604)
                     /gene="tcdC"
                     /locus_tag="PC429_002107"
     CDS             complement(17906..18604)
                     /gene="tcdC"
                     /locus_tag="PC429_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF3847611.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIALLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            19030..19275
                     /locus_tag="PC429_002108"
     CDS             19030..19275
                     /locus_tag="PC429_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF3847612.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19493..19762)
                     /locus_tag="PC429_002109"
     CDS             complement(19493..19762)
                     /locus_tag="PC429_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF3847613.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT         8073 a         2285 c         3077 g         6926 t
ORIGIN      
        1 actttattta ttagaaaaag attactaatt aattattaaa attaatgtat taataatgca
       61 tattttcata taaaatttaa tttatttgcc gattatataa ttataatgac tgatttaatt
      121 ccgatgttgt caaaattttc aaataaatca tcattataaa tataagagag gatgatttta
      181 tgcaaaagtc tttttatgaa ttaattgttt tagcaagaaa taactcagta gatgatttgc
      241 aagaaatttt atttatgttt aagccattag taaaaaagct tagtagagtt ttacattatg
      301 aagagggaga aacagattta ataatatttt ttattgaatt aataaaaaat attaaattaa
      361 gtagcttttc agaaaaaagc gatgctatta tagtcaaata tattcataaa tcattactga
      421 ataagacttt tgagttgtct agaagatatt ctaaaatgaa gtttaatttt gtagaatttg
      481 atgaaaatat cttaaatatg aaaaataatt atcaaagtaa gtctattttt gaggaagata
      541 tttgtttttt cggatatatt ttgaaagaat tatctggtat tcaaagaaaa gttatttttt
      601 ataaatattt aaaaggatat tctgatagag aaatatcagt gaaattaaaa atatctagac
      661 aagctgttaa taaggctaaa aatagagcat ttaaaaaaat aaaaaaagac tatgaaaatt
      721 attttaactt gtaattaatg agcttaaaga aatatttaca atagaaatca aattttagaa
      781 ttaactttat tgtaaaatca ataacttaat ctaagaatat cttaattttt atattttata
      841 tagaacaaag tttacatatt tatttcagac aacgtcttta ttcaatcgaa gagcaaatta
      901 atcaactgag tgtcttcaat ttaaaatgtt aggaagtgaa tgtatatgaa aacctaagta
      961 gatattagta tattttataa atagaaagga ggatatataa aagagtttta tcatttagat
     1021 gtaaaaatat tatagtaaag gagaaaattt tatgagttta gttaatagaa aacagttaga
     1081 aaaaatggca aatgtaagat ttcgtactca agaagatgaa tatgttgcaa tattggatgc
     1141 tttagaagaa tatcataata tgtcagagaa tactgtagtc gaaaaatatt taaaattaaa
     1201 agatataaat agtttaacag atatttatat agatacatat aaaaaatctg gtagaaataa
     1261 agccttaaaa aaatttaagg aatatctagt tacagaagta ttagagctaa agaataataa
     1321 tttaactcca gttgagaaaa atttacattt tgtttggatt ggaggtcaaa taaatgacac
     1381 tgctattaat tatataaatc aatggaaaga tgtaaatagt gattataatg ttaatgtttt
     1441 ttatgatagt aatgcatttt tgataaacac attgaaaaaa actgtagtag aatcagcaat
     1501 aaatgataca cttgaatcat ttagagaaaa cttaaatgac cctagatttg actataataa
     1561 attcttcaga aaacgtatgg aaataattta tgataaacag aaaaatttca taaactacta
     1621 taaagctcaa agagaagaaa atcctgaact tataattgat gatattgtaa agacatatct
     1681 ttcaaatgag tattcaaagg agatagatga acttaatacc tatattgaag aatccttaaa
     1741 taaaattaca cagaatagtg gaaatgatgt tagaaacttt gaagaattta aaaatggaga
     1801 gtcattcaac ttatatgaac aagagttggt agaaaggtgg aatttagctg ctgcttctga
     1861 catattaaga atatctgcat taaaagaaat tggtggtatg tatttagatg ttgatatgtt
     1921 accaggaata caaccagact tatttgagtc tatagagaaa cctagttcag taacagtgga
     1981 tttttgggaa atgacaaagt tagaagctat aatgaaatac aaagaatata taccagaata
     2041 tacctcagaa cattttgaca tgttagacga agaagttcaa agtagttttg aatctgttct
     2101 agcttctaag tcagataaat cagaaatatt ctcatcactt ggtgatatgg aggcatcacc
     2161 actagaagtt aaaattgcat ttaatagtaa gggtattata aatcaagggc taatttctgt
     2221 gaaagactca tattgtagca atttaatagt aaaacaaatc gagaatagat ataaaatatt
     2281 gaataatagt ttaaatccag ctattagcga ggataatgat tttaatacta caacgaatac
     2341 ctttattgat agtataatgg ctgaagctaa tgcagataat ggtagattta tgatggaact
     2401 aggaaagtat ttaagagttg gtttcttccc agatgttaaa actactatta acttaagtgg
     2461 ccctgaagca tatgcggcag cttatcaaga tttattaatg tttaaagaag gcagtatgaa
     2521 tatccatttg atagaagctg atttaagaaa ctttgaaatc tctaaaacta atatttctca
     2581 atcaactgaa caagaaatgg ctagcttatg gtcatttgac gatgcaagag ctaaagctca
     2641 atttgaagaa tataaaagga attattttga aggttctctt ggtgaagatg ataatcttga
     2701 tttttctcaa aatatagtag ttgacaagga gtatctttta gaaaaaatat cttcattagc
     2761 aagaagttca gagagaggat atatacacta tattgttcag ttacaaggag ataaaattag
     2821 ttatgaagca gcatgtaact tatttgcaaa gactccttat gatagtgtac tatttcagaa
     2881 aaatatagaa gattcagaaa ttgcatatta ttataatcct ggagatggtg aaatacaaga
     2941 aatagacaag tataaaattc caagtataat ttctgataga cctaagatta aattaacatt
     3001 tattggtcat ggtaaagatg aatttaatac tgatatattt gcaggttttg atgtagattc
     3061 attatccaca gaaatagaag cagcaataga tttagctaaa gaggatattt ctcctaagtc
     3121 aatagaaata aatttattag gatgtaatat gtttagctac tctatcaacg tagaggagac
     3181 ttatcctgga aaattattac ttaaagttaa agataaaata tcagaattaa tgccatctat
     3241 aagtcaagac tctattatag taagtgcaaa tcaatatgaa gttagaataa atagtgaagg
     3301 aagaagagaa ttattggatc attctggtga atggataaat aaagaagaaa gtattataaa
     3361 ggatatttca tcaaaagaat atatatcatt taatcctaaa gaaaataaaa ttacagtaaa
     3421 atctaaaaat ttacctgagc tatctacatt attacaagaa attagaaata attctaattc
     3481 aagtgatatt gaactagaag aaaaagtaat gttaacagaa tgtgagataa atgttatttc
     3541 aaatatagat acgcaaattg ttgaggaaag gattgaagaa gctaagaatt taacttctga
     3601 ctctattaat tatataaaag atgaatttaa actaatagaa tctatttctg atgcactatg
     3661 tgacttaaaa caacagaatg aattagaaga ttctcatttt atatcttttg aggacatatc
     3721 agagactgat gagggattta gtataagatt tattaataaa gaaactggag aatctatatt
     3781 tgtagaaact gaaaaaacaa tattctctga atatgctaat catataactg aagagatttc
     3841 taagataaaa ggtactatat ttgatactgt aaatggtaag ttagtaaaaa aagtaaattt
     3901 agatactaca cacgaagtaa atactttaaa tgctgcattt tttatacaat cattaataga
     3961 atataatagt tctaaagaat ctcttagtaa tttaagtgta gcaatgaaag tccaagttta
     4021 cgctcaatta tttagtactg gtttaaatac tattacagat gcagccaaag ttgttgaatt
     4081 agtatcaact gcattagatg aaactataga cttacttcct acattatctg aaggattacc
     4141 tataattgca actattatag atggtgtaag tttaggtgca gcaatcaaag agctaagtga
     4201 aacgagtgac ccattattaa gacaagaaat agaagctaag ataggtataa tggcagtaaa
     4261 tttaacaaca gctacaactg caatcattac ttcatctttg ggggtagcta gtggatttag
     4321 tatactttta gttcctttag caggaatttc agcaggtata ccaagcttag taaacaatga
     4381 acttgtactt cgagataagg caacaaaggt tgtagattat tttaaacatg tttcattagt
     4441 tgaaactgaa ggagtattta ctttattaga tgataaaata atgatgccac aagatgattt
     4501 agtgatatca gaaatagatt ttaataataa ttcaatagtt ttaggtaaat gtgaaatctg
     4561 gagaatggaa ggtggttcag gtcatactgt aactgatgat atagatcact tcttttcagc
     4621 accatcaata acatatagag agccacactt atctatatat gacgtattgg aagtacaaaa
     4681 agaagaactt gatttgtcaa aagatttaat ggtattacct aatgctccaa atagagtatt
     4741 tgcttgggaa acaggatgga caccaggttt aagaagctta gaaaatgatg gcacaaaact
     4801 gttagaccgt ataagagata actatgaagg tgagttttat tggagatatt ttgcttttat
     4861 agctgatgct ttaataacaa cattaaaacc aagatatgaa gatactaata taagaataaa
     4921 tttagatagt aatactagaa gttttatagt tccaataata actacagaat atataagaga
     4981 aaaattatca tattctttct atggttcagg aggaacttat gcattgtctc tttctcaata
     5041 taatatgggt ataaatatag aattaagtga aagtgatgtt tggattatag atgttgataa
     5101 tgttgtgaga gatgtaacta tagaatctga taaaattaaa aaaggtgatt taatagaagg
     5161 tattttatct acactaagta ttgaagagaa taaaattatc ttaaatagcc atgagattaa
     5221 tttttctggt gaggtaaatg gaagtaatgg atttgtttct ttaacatttt caattttaga
     5281 aggaataaat gcaattatag aagttgattt attatctaaa tcatataaat tacttatttc
     5341 tggcgaatta aaaatattga tgttaaattc aaatcatatt caacagaaaa tagattatat
     5401 aggattcaat agcgaattac agaaaaatat accatatagc tttgtagata gtgaaggaaa
     5461 agagaatggt tttattaatg gttcaacaaa agaaggttta tttgtatctg aattacctga
     5521 tgtagttctt ataagtaagg tttatatgga tgatagtaag ccttcatttg gatattatag
     5581 taataatttg aaagatgtca aagttataac taaagataat gttaatatat taacaggtta
     5641 ttatcttaag gatgatataa aaatctctct ttctttgact ctacaagatg aaaaaactat
     5701 aaagttaaat agtgtgcatt tagatgaaag tggagtagct gagattttga agttcatgaa
     5761 tagaaaaggt aatacaaata cttcagattc tttaatgagc tttttagaaa gtatgaatat
     5821 aaaaagtatt ttcgttaatt tcttacaatc taatattaag tttatattag atgctaattt
     5881 tataataagt ggtactactt ctattggcca atttgagttt atttgtgatg aaaatgataa
     5941 tatacaacca tatttcatta agtttaatac actagaaact aattatactt tatatgtagg
     6001 aaatagacaa aatatgatag tggaaccaaa ttatgattta gatgattctg gagatatatc
     6061 ttcaactgtt atcaatttct ctcaaaagta tctttatgga atagacagtt gtgttaataa
     6121 agttgtaatt tcaccaaata tttatacaga tgaaataaat ataacgcctg tatatgaaac
     6181 aaataatact tatccagaag ttattgtatt agatgcaaat tatataaatg aaaaaataaa
     6241 tgttaatatc aatgatctat ctatacgata tgtatggagt aatgatggta atgattttat
     6301 tcttatgtca actagtgaag aaaataaggt gtcacaagtt aaaataagat tcgttaatgt
     6361 ttttaaagat aagactttgg caaataagct atcttttaac tttagtgata aacaagatgt
     6421 acctgtaagt gaaataatct tatcatttac accttcatat tatgaggatg gattgattgg
     6481 ctatgatttg ggtctagttt ctttatataa tgagaaattt tatattaata actttggaat
     6541 gatggtatct ggattaatat atattaatga ttcattatat tattttaaac caccagtaaa
     6601 taatttgata actggatttg tgactgtagg cgatgataaa tactacttta atccaattaa
     6661 tggtggagct gcttcaattg gagagacaat aattgatgac aaaaattatt atttcaacca
     6721 aagtggagtg ttacaaacag gtgtatttag tacagaagat ggatttaaat attttgcccc
     6781 agctaataca cttgatgaaa acctagaagg agaagcaatt gattttactg gaaaattaat
     6841 tattgacgaa aatatttatt attttgatga taattataga ggagctgtag aatggaaaga
     6901 attagatggt gaaatgcact attttagccc agaaacaggt aaagctttta aaggtctaaa
     6961 tcaaataggt gattataaat actatttcaa ttctgatgga gttatgcaaa aaggatttgt
     7021 tagtataaat gataataaac actattttga tgattctggt gttatgaaag taggttacac
     7081 tgaaatagat ggcaagcatt tctactttgc tgaaaacgga gaaatgcaaa taggagtatt
     7141 taatacagaa gatggattta aatattttgc tcatcataat gaagatttag gaaatgaaga
     7201 aggtgaagaa atctcatatt ctggtatatt aaatttcaat aataaaattt actattttga
     7261 tgattcattt acagctgtag ttggatggaa agatttagag gatggttcaa agtattattt
     7321 tgatgaagat acagcagaag catatatagg tttgtcatta ataaatgatg gtcaatatta
     7381 ttttaatgat gatggaatta tgcaagttgg atttgtcact ataaatgata aagtcttcta
     7441 cttctctgac tctggaatta tagaatctgg agtacaaaac atagatgaca attatttcta
     7501 tatagatgat aatggtatag ttcaaattgg tgtatttgat acttcagatg gatataaata
     7561 ttttgcacct gctaatactg taaatgataa tatttacgga caagcagttg aatatagtgg
     7621 tttagttaga gttggggaag atgtatatta ttttggagaa acatatacaa ttgagactgg
     7681 atggatatat gatatggaaa atgaaagtga taaatattat ttcaatccag aaactaaaaa
     7741 agcatgcaaa ggtattaatt taattgatga tataaaatat tattttgatg agaagggcat
     7801 aatgagaacg ggtcttatat catttgaaaa taataattat tactttaatg agaatggtga
     7861 aatgcaattt ggttatataa atatagaaga taagatgttc tattttggtg aagatggtgt
     7921 catgcagatt ggagtattta atacaccaga tggatttaaa tactttgcac atcaaaatac
     7981 tttggatgag aattttgagg gagaatcaat aaactatact ggttggttag atttagatga
     8041 aaagagatat tattttacag atgaatatat tgcagcaact ggttcagtta ttattgatgg
     8101 tgaggagtat tattttgatc ctgatacagc tcaattagtg attagtgaat agataaaaat
     8161 atgttaaata tatcctctta tacttaaata tataaaaata aacaaaatga tacactacat
     8221 aaagtgttct atctaatatg aagatttacc aataaaaagg tggactatga tgaatgcaca
     8281 gtagttcacc tttttatatt tctaatggta acaaaatatt tttttatata aacctaggag
     8341 gcgttatgaa tatgacaata tcttttttat cagagcatat atttataaag ttagtaattt
     8401 taactatatc atttgataca ttattaggat gtttaagtgc aataaaaagt cgtaaattta
     8461 attctagttt tggaatagat ggaggaatca gaaaagtagc aatgatagca tgtatatttt
     8521 ttttatcagt agttgacatt cttacaaagt ttaacttttt atttatgtta ccacaagatt
     8581 gtatcaattt tttaagacta aaacatcttg gaatatctga atttttctct attttattta
     8641 ttttatatga aagtgtaagt atattaaaaa atatgtgctt atgtggatta ccagtaccta
     8701 agagattaaa ggaaaaaata gcaattttac tagatgcaat gacagatgaa atgaatgcta
     8761 aggatgaaaa gtaagtaatg gtagatataa taaagatatt aacaaataaa aagtgttatc
     8821 caaataagaa tagctgaaag ttatcataat tcatgaaact aataatgaaa acgagggagc
     8881 agatgccaag agacacacaa gtattaaata catataattt cgaagcaagt gttcattact
     8941 atatagatga caaggtagta tatcaaacat tggttcacaa agatggtgca tggtcagttg
     9001 gtaaaatcta ttaagctaca ttagttacag atatcacaaa ctataatagt taaacataga
     9061 aatatgtgta aattgtgatg gaaattattc aaaaacacaa aaatacgtga tgaaggacaa
     9121 aatgatatag aaaataagta tcaaacctta ataaatgatt taattgatag tttaaaagtt
     9181 ataggaaaaa tatataaaga aataaaaaca ttaaaaaaat ataagatatg tttacaaatt
     9241 actatcagac aatctcctta tctaatagaa gagtcaatta actaattgag tatctttaaa
     9301 ttgaaatgtt aggaagtgat ttaaatatga aaacttaaat tataaaaaat caatattaat
     9361 ttatttttaa aaaatagaaa ggagtgtata agatttattt tcaaagttta aaaacaagaa
     9421 aatcaattta aatttcagaa ggaataaatg tggttataga agtggattta ttatcaaaaa
     9481 taataatact aggaggtttt tatgtcttta atatctaaag aagagttaat aaaactcgca
     9541 tatagcatta gaccaagaga aaatgagtat aaaactatac taactaattt agacgaatat
     9601 aataagttaa ctacaaacaa taatgaaaat aaatatttac aattaaaaaa actaaatgaa
     9661 tcaattgatg tttttatgaa taaatataaa acttcaagca gaaatagagc actctctaat
     9721 ctaaaaaaag atatattaaa agaagtaatt cttattaaaa attccaatac aagccctgta
     9781 gaaaaaaatt tacattttgt atggataggt ggagaagtca gtgatattgc tcttgaatac
     9841 ataaaacaat gggctgatat taatgcagaa tataatatta aactgtggta tgatagtgaa
     9901 gcattcttag taaatacact aaaaaaggct atagttgaat cttctaccac tgaagcatta
     9961 cagctactag aggaagagat tcaaaatcct caatttgata atatgaaatt ttacaaaaaa
    10021 aggatggaat ttatatatga tagacaaaaa aggtttataa attattataa atctcaaatc
    10081 aataaaccta cagtacctac aatagatgat attataaagt ctcatctagt atctgaatat
    10141 aatagagatg aaactgtatt agaatcatat agaacaaatt ctttgagaaa aataaatagt
    10201 aatcatggta tagatatcag ggctaatagt ttgtttacag aacaagagtt attaaatatt
    10261 tatagtcagg agttgttaaa tcgtggaaat ttagctgcag catctgacat agtaagatta
    10321 ttagccctaa aaaattttgg cggagtatat ttagatgttg atatgcttcc aggtattcac
    10381 tctgatttat ttaaaacaat atctagacct agctctattg gactagaccg ttgggaaatg
    10441 ataaaattag aggctattat gaagtataaa aaatatataa ataattatac atcagaaaac
    10501 tttgataaac ttgatcaaca attaaaagat aattttaaac tcattataga aagtaaaagt
    10561 gaaaaatctg agatattttc taaattagaa aatttaaatg tatctgatct tgaaattaaa
    10621 atagctttcg ctttaggcag tgttataaat caagccttga tatcaaaaca aggttcatat
    10681 cttactaacc tagtaataga acaagtaaaa aatagatatc aatttttaaa ccaacacctt
    10741 aacccagcca tagagtctga taataacttc acagatacta ctaaaatttt tcatgattca
    10801 ttatttaatt cagctaccgc agaaaactct atgtttttaa caaaaatagc accatactta
    10861 caagtaggtt ttatgccaga agctcgctcc acaataagtt taagtggtcc aggagcttat
    10921 gcgtcagctt actatgattt cataaattta caagaaaata ctatagaaaa aactttaaaa
    10981 gcatcagatt taatagaatt taaattccca gaaaataatc tatctcaatt gacagaacaa
    11041 gaaataaata gtctatggag ctttgatcaa gcaagtgcaa aatatcaatt tgagaaatat
    11101 gtaagagatt atactggtgg atctctttct gaagacaatg gggtagactt taataaaaat
    11161 actgccctcg acaaaaacta tttattaaat aataaaattc catcaaacaa tgtagaagaa
    11221 gctggaagta aaaattatgt tcattatatc atacagttac aaggagatga tataagttat
    11281 gaagcaacat gcaatttatt ttctaaaaat cctaaaaata gtattattat acaacgaaat
    11341 atgaatgaaa gtgcaaaaag ctacttttta agtgatgatg gagaatctat tttagaatta
    11401 aataaatata ggatacctga aagattaaaa aataaggaaa aagtaaaagt aacctttatt
    11461 ggacatggta aagatgaatt caacacaagc gaatttgcta gattaagtgt agattcactt
    11521 tccaatgaga taagttcatt tttagatacc ataaaattag atatatcacc taaaaatgta
    11581 gaagtaaact tacttggatg taatatgttt agttatgatt ttaatgttga agaaacttat
    11641 cctgggaagt tgctattaag tattatggac aaaattactt ccactttacc tgatgtaaat
    11701 aaaaattcta ttactatagg agcaaatcaa tatgaagtaa gaattaatag tgagggaaga
    11761 aaagaacttc tggctcactc aggtaaatgg ataaataaag aagaagctat tatgagcgat
    11821 ttatctagta aagaatacat tttttttgat tctatagata ataagctaaa agcaaagtcc
    11881 aagaatattc caggattagc atcaatatca gaagatataa aaacattatt acttgatgca
    11941 agtgttagtc ctgatacaaa atttatttta aataatctta agcttaatat tgaatcttct
    12001 attggtgatt acatttatta tgaaaaatta gagcctgtta aaaatataat tcacaattct
    12061 atagatgatt taatagatga gttcaatcta cttgaaaatg tatctgatga attatatgaa
    12121 ttaaaaaaat taaataatct agatgagaag tatttaatat cttttgaaga tatctcaaaa
    12181 aataattcaa cttactctgt aagatttatt aacaaaagta atggtgagtc agtttatgta
    12241 gaaacagaaa aagaaatttt ttcaaaatat agcgaacata ttacaaaaga aataagtact
    12301 ataaagaata gtataattac agatgttaat ggtaatttat tggataatat acagttagat
    12361 catacttctc aagttaatac attaaacgca gcattcttta ttcaatcatt aatagattat
    12421 agtagcaata aagatgtact gaatgattta agtacctcag ttaaggttca actttatgct
    12481 caactattta gtacaggttt aaatactata tatgactcta tccaattagt aaatttaata
    12541 tcaaatgcag taaatgatac tataaatgta ctacctacaa taacagaggg gatacctatt
    12601 gtatctacta tattagacgg aataaactta ggtgcagcaa ttaaggaatt actagacgaa
    12661 catgacccat tactaaaaaa agaattagaa gctaaggtgg gtgttttagc aataaatatg
    12721 tcattatcta tagctgcaac tgtagcttca attgttggaa taggtgctga agttactatt
    12781 ttcttattac ctatagctgg tatatctgca ggaatacctt cattagttaa taatgaatta
    12841 atattgcatg ataaggcaac ttcagtggta aactatttta atcatttgtc tgaatctaaa
    12901 aaatatggcc ctcttaaaac agaagatgat aaaattttag ttcctattga tgatttagta
    12961 atatcagaaa tagattttaa taataattcg ataaaactag gaacatgtaa tatattagca
    13021 atggaggggg gatcaggaca cacagtgact ggtaatatag atcacttttt ctcatctcca
    13081 tctataagtt ctcatattcc ttcattatca atttattctg caataggtat agaaacagaa
    13141 aatctagatt tttcaaaaaa aataatgatg ttacctaatg ctccttcaag agtgttttgg
    13201 tgggaaactg gagcagttcc aggtttaaga tcattggaaa atgacggaac tagattactt
    13261 gattcaataa gagatttata cccaggtaaa ttttactgga gattctatgc ttttttcgat
    13321 tatgcaataa ctacattaaa accagtttat gaagacacta atattaaaat taaactagat
    13381 aaagatacta gaaacttcat aatgccaact ataactacta acgaaattag aaacaaatta
    13441 tcttattcat ttgatggagc aggaggaact tactctttat tattatcttc atatccaata
    13501 tcaacgaata taaatttatc taaagatgat ttatggatat ttaatattga taatgaagta
    13561 agagaaatat ctatagaaaa tggtactatt aaaaaaggaa agttaataaa agatgtttta
    13621 agtaaaattg atataaataa aaataaactt attataggca atcaaacaat agatttttca
    13681 ggcgatatag ataataaaga tagatatata ttcttgactt gtgagttaga tgataaaatt
    13741 agtttaataa tagaaataaa tcttgttgca aaatcttata gtttgttatt gtctggggat
    13801 aaaaattatt tgatatccaa tttatctaat actattgaga aaatcaatac tttaggccta
    13861 gatagtaaaa atatagcgta caattacact gatgaatcta ataataaata ttttggagct
    13921 atatctaaaa caagtcaaaa aagcataata cattataaaa aagacagtaa aaatatatta
    13981 gaattttata atgacagtac attagaattt aacagtaaag attttattgc tgaagatata
    14041 aatgtattta tgaaagatga tattaatact ataacaggaa aatactatgt tgataataat
    14101 actgataaaa gtatagattt ctctatttct ttagttagta aaaatcaagt aaaagtaaat
    14161 ggattatatt taaatgaatc cgtatactca tcttaccttg attttgtgaa aaattcagat
    14221 ggacaccata atacttctaa ttttatgaat ttatttttgg acaatataag tttctggaaa
    14281 ttgtttgggt ttgaaaatat aaattttgta atcgataaat actttaccct tgttggtaaa
    14341 actaatcttg gatatgtaga atttatttgt gacaataata aaaatataga tatatatttt
    14401 ggtgaatgga aaacatcgtc atctaaaagc actatattta gcggaaatgg tagaaatgtt
    14461 gtagtagagc ctatatataa tcctgatacg ggtgaagata tatctacttc actagatttt
    14521 tcctatgaac ctctctatgg aatagataga tatatcaata aagtattgat agcacctgat
    14581 ttatatacaa gtttaataaa tattaatacc aattattatt caaatgagta ctaccctgag
    14641 attatagttc ttaacccaaa tacattccac aaaaaagtaa atataaattt agatagttct
    14701 tcttttgagt ataaatggtc tacagaagga agtgacttta ttttagttag atacttagaa
    14761 gaaagtaata aaaaaatatt acaaaaaata agaatcaaag gtatcttatc taatactcaa
    14821 tcatttaata aaatgagtat agattttaaa gatattaaaa aactatcatt aggatatata
    14881 atgagtaatt ttaaatcatt taattctgaa aatgaattag atagagatca tttaggattt
    14941 aaaataatag ataataaaac ttattactat gatgaagata gtaaattagt taaaggatta
    15001 atcaatataa ataattcatt attctatttt gatcctatag aatttaactt agtaactgga
    15061 tggcaaacta tcaatggtaa aaaatattat tttgatataa atactggagc agctttaact
    15121 agttataaaa ttattaatgg taaacacttt tattttaata atgatggtgt gatgcagttg
    15181 ggagtattta aaggacctga tggatttgaa tattttgcac ctgccaatac tcaaaataat
    15241 aacatagaag gtcaggctat agtttatcaa agtaaattct taactttgaa tggcaaaaaa
    15301 tattattttg ataatgactc aaaagcagtc actggatgga gaattattaa caatgagaaa
    15361 tattacttta atcctaataa tgctattgct gcagtcggat tgcaagtaat tgacaataat
    15421 aagtattatt tcaatcctga cactgctatc atctcaaaag gttggcagac tgttaatggt
    15481 agtagatact actttgatac tgataccgct attgccttta atggttataa aactattgat
    15541 ggtaaacact tttattttga tagtgattgt gtagtgaaaa taggtgtgtt tagtacctct
    15601 aatggatttg aatattttgc acctgctaat acttataata ataacataga aggtcaggct
    15661 atagtttatc aaagtaaatt cttaactttg aatggtaaaa aatattactt tgataataac
    15721 tcaaaagcag ttaccggatg gcaaactatt gatagtaaaa aatattactt taatactaac
    15781 actgctgaag cagctactgg atggcaaact attgatggta aaaaatatta ctttaatact
    15841 aacactgctg aagcagctac tggatggcaa actattgatg gtaaaaaata ttactttaat
    15901 actaacactg ctatagcttc aactggttat acaattatta atggtaaaca tttttatttt
    15961 aatactgatg gtattatgca gataggagtg tttaaaggac ctaatggatt tgaatatttt
    16021 gcacctgcta atacggatgc taacaacata gaaggtcaag ctatacttta ccaaaatgaa
    16081 ttcttaactt tgaatggtaa aaaatattac tttggtagtg actcaaaagc agttactgga
    16141 tggagaatta ttaacaataa gaaatattac tttaatccta ataatgctat tgctgcaatt
    16201 catctatgca ctataaataa tgacaagtat tactttagtt atgatggaat tcttcaaaat
    16261 ggatatatta ctattgaaag aaataatttc tattttgatg ctaataatga atctaaaatg
    16321 gtaacaggag tatttaaagg acctaatgga tttgagtatt ttgcacctgc taatactcac
    16381 aataataaca tagaaggtca ggctatagtt taccagaaca aattcttaac tttgaatggc
    16441 aaaaaatatt attttgataa tgactcaaaa gcagttactg gatggcaaac cattgatggt
    16501 aaaaaatatt actttaatct taacactgct gaagcagcta ctggatggca aactattgat
    16561 ggtaaaaaat attactttaa tactaacact ttcatagcct caactggtta tacaagtatt
    16621 aatggtaaac atttttattt taatactgat ggtattatgc agataggagt gtttaaagga
    16681 cctaatggat ttgaatactt tgcacctgct aatactcata ataataacat agaaggtcaa
    16741 gctatacttt accaaaataa attcttaact ttgaatggta aaaaatatta ctttggtagt
    16801 gactcaaaag cagttaccgg attgcgaact attgatggta aaaaatatta ctttaatact
    16861 aacactgctg ttgcagttac tggatggcaa actattaatg gtaaaaaata ctactttaat
    16921 actaacactt ctatagcttc aactggttat acaattatta gtggtaaaca tttttatttt
    16981 aatactgatg gtattatgca gataggagtg tttaaaggac ctgatggatt tgaatacttt
    17041 gcacctgcta atacagatgc taacaatata gaaggtcaag ctatacgtta tcaaaataga
    17101 ttcctatatt tacatgacaa tatatattat tttggtaata attcaaaagc ggctactggt
    17161 tgggtaacta ttgatggtaa tagatattac ttcgagccta atacagctat gggtgcgaat
    17221 ggttataaaa ctattgataa taaaaatttt tactttagaa atggtttacc tcagataggg
    17281 gtgtttaaag ggtctaatgg atttgaatac tttgcacctg ctaatacgga tgctaacaat
    17341 atagaaggtc aagctatacg ttatcaaaat agattcctac atttacttgg aaaaatatat
    17401 tactttggta ataattcaaa agcagttact ggatggcaaa ctattaatgg taaagtatat
    17461 tactttatgc ctgatactgc tatggctgca gctggtggac tttttgagat tgatggtgtt
    17521 atatatttct ttggtgttga tggagtaaaa gcccctggga tatatggcta aaatatatgt
    17581 ttgataaaaa attattcctg tgctactaag aaattatttt tatataataa atattgagat
    17641 ttaattaaaa gtcatgtgtt attgtaatac atgactttta gttaaaattt ttctatcatt
    17701 taataatcta ttattcttga ctattttata ataaaattca tatatggaaa tattaatact
    17761 aaataattaa tagttgataa aaaatagata atatgctaaa agcaaaaact aatttagagc
    17821 cttgtaactg tttatttgca attataaaaa catctttaaa catattgact ataatataaa
    17881 atattaacta taatacaaaa caatattaat taattttctc tacagctatc cctggtatag
    17941 ttatatttcc acccatagtt gattcataag ttatagttcc agcacttata cctcttatag
    18001 ttatgtaatc atcctctaat actcttgaag gagttattga ttttttataa ctacatagta
    18061 caaccttatc ataatttcca gacacagcta atcttatttg cacctcatca ccatcttcaa
    18121 taacttgaat aaccttacct tcaaatttta cctttttgta cttataatca tcaggtgttc
    18181 tagctaattg gtcataagta ataccagtat catatccttt cttctcctct tcttcttttt
    18241 tgcgttgttc ttcagccttt ttagcttctt cagctttttt agcttcttca gctttacgtt
    18301 gattttcagc ttcaatagct ttcttttcgt cgtctttcat tttgaaccat ggttcagcat
    18361 cagacaattt gctatttaaa gtttctattt ctgttttttg agaatctata actgatgttt
    18421 ggttagaaat gacctcctca tggtcttcag aacaagctgg tgaggatata ttgccaaaaa
    18481 acacgcctaa aataaatgcc agtagagcaa taccctttgt gctcttaaag aatttaatta
    18541 ttttcttaga gcttcctttt ccttcattac taaattcgtt accatcattt tttttagaaa
    18601 acataataca atctcccttt tattagatat attttatttt aaaagttaaa aacttaaaat
    18661 aactagttga ctaaaaatta tatattcata ttttcctaaa atatatttcc cctaagcata
    18721 attttacaaa ataaagtaag aagattcaat atattttgaa aaaataagat aaaaatgtca
    18781 gattataata taattttctt gaaggtgaat tttatatatt aaattaaaca taaagtattt
    18841 tatttgtata aaactatatt atgatgtaat taagtgactc agtgatttag aggtggtaaa
    18901 atatatatat atgaaagagc ataaataaat tgatataata aaaaattaaa tattaaatat
    18961 taactataat aaaaaaatat ctggtaatag gtataatata aaaagaatat accagaaaga
    19021 aggtgaagta tgggagaact acaaaaaatt ccaggggtag ggaaagcaac agaaaaatct
    19081 ttaatcatgt taggatatac aactattaaa tcacttaaag atgcaaaccc agcacaaatg
    19141 tatgaaaagg aatgtttaat acgtggacag cacatagaca gatgtcagtt atatgtatac
    19201 agatgtgcag tatattttgc atcaactgaa aatccagaac cagaaaaatt aaagtggtgg
    19261 tattggaaag attaaaaaat caaaaaagtt agacataaaa aagagttgaa tatccgtttt
    19321 aaataataaa aactaatact caactctttt ttagtcgtaa attttatgta aatctatttg
    19381 tatggctaaa tatacccatc aataaatgta taaatatacc aatcaacaaa tgactaaaca
    19441 tactacctaa caaaggatta aataaattac ttaacaaagc attctaaaac aattaattag
    19501 aaactgtttc ttcaagccat tttctacatt gctcaatatt taccataggg tcaaaacctt
    19561 ggtcttttcc cttatttaaa agcatatcac aagggaaagt atcacattca ccacaatgat
    19621 taagttcctt actttcacag caagttttaa ccttacattc tccaccccaa aatggttttt
    19681 ccatagttgg gcaaccagtg caatttactt gttctttttt tgtacaactg tcacaacaga
    19741 caccacatct tgattcaaac atgttcaaac atcctttcca ttatttaaaa atattataaa
    19801 atgattttag atgaatacaa aagtgcaaat agttgatttt aggtcatcta aatgttaaac
    19861 ataaaattat tagtactggt aaatttataa aaactattcc aaaaatcttt ttgaatattt
    19921 caaaacacaa taaattactt caaatccctt tgaatatatt ttacagcatt atagattgct
    19981 ttaaatctct ttgaatattc tacaagacta tagattagtt taaatccctt tgaatatatt
    20041 ttacaacatt atagattgtt ttaaatctct ttgaatattc tacaagacta tagattagtt
    20101 taaatccctt tgaatatttc aaaacaccat agattacttt aaatcccttt gaatattcta
    20161 taacactata aattactttt aacttttata taaccataaa gtttttatct ggcactttaa
    20221 atggagtttt atatcaaatt tgccttccat cgaataacca aacttaattt aattacacat
    20281 tccaagagtc aaatattcat tcaatatatt tgataattct atgttatagc tataattaaa
    20341 tatagattat taaagttaaa t
//
LOCUS       DAMKMI010000064        20306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMKMI010000064 DAMKMI010000000
VERSION     DAMKMI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.065"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(47..925)
                     /locus_tag="PC429_002110"
     CDS             complement(47..925)
                     /locus_tag="PC429_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3847614.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            complement(942..1259)
                     /gene="trxA"
                     /locus_tag="PC429_002111"
     CDS             complement(942..1259)
                     /gene="trxA"
                     /locus_tag="PC429_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF3847615.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            complement(1409..2641)
                     /locus_tag="PC429_002112"
     CDS             complement(1409..2641)
                     /locus_tag="PC429_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3847616.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            complement(2631..3302)
                     /locus_tag="PC429_002113"
     CDS             complement(2631..3302)
                     /locus_tag="PC429_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847617.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            complement(3318..4154)
                     /locus_tag="PC429_002114"
     CDS             complement(3318..4154)
                     /locus_tag="PC429_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847618.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            complement(4180..4854)
                     /locus_tag="PC429_002115"
     CDS             complement(4180..4854)
                     /locus_tag="PC429_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF3847619.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYIFKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            complement(4868..5596)
                     /locus_tag="PC429_002116"
     CDS             complement(4868..5596)
                     /locus_tag="PC429_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF3847620.1"
                     /translation="MMFDRVKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            complement(5723..6412)
                     /locus_tag="PC429_002117"
     CDS             complement(5723..6412)
                     /locus_tag="PC429_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3847621.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            complement(6420..6983)
                     /locus_tag="PC429_002118"
     CDS             complement(6420..6983)
                     /locus_tag="PC429_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3847622.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            complement(6985..7953)
                     /locus_tag="PC429_002119"
     CDS             complement(6985..7953)
                     /locus_tag="PC429_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF3847623.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     regulatory      complement(8054..8097)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            8462..9091
                     /locus_tag="PC429_002120"
     CDS             8462..9091
                     /locus_tag="PC429_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3847624.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMYSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     gene            9066..9470
                     /locus_tag="PC429_002121"
     CDS             9066..9470
                     /locus_tag="PC429_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF3847625.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            9473..9946
                     /locus_tag="PC429_002122"
     CDS             9473..9946
                     /locus_tag="PC429_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF3847626.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            10516..11181
                     /locus_tag="PC429_002123"
     CDS             10516..11181
                     /locus_tag="PC429_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF3847627.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            11203..12135
                     /locus_tag="PC429_002124"
     CDS             11203..12135
                     /locus_tag="PC429_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF3847628.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            12156..12797
                     /gene="pcp"
                     /locus_tag="PC429_002125"
     CDS             12156..12797
                     /gene="pcp"
                     /locus_tag="PC429_002125"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF3847629.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(12917..13249)
                     /locus_tag="PC429_002126"
     CDS             complement(12917..13249)
                     /locus_tag="PC429_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847630.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            13515..14135
                     /locus_tag="PC429_002127"
     CDS             13515..14135
                     /locus_tag="PC429_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3847631.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNKLIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(14460..15284)
                     /locus_tag="PC429_002128"
     CDS             complement(14460..15284)
                     /locus_tag="PC429_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF3847632.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(15295..16698)
                     /locus_tag="PC429_002129"
     CDS             complement(15295..16698)
                     /locus_tag="PC429_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3847633.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            complement(16702..17427)
                     /locus_tag="PC429_002130"
     CDS             complement(16702..17427)
                     /locus_tag="PC429_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847634.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLFAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            complement(17539..18312)
                     /locus_tag="PC429_002131"
     CDS             complement(17539..18312)
                     /locus_tag="PC429_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847635.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            complement(18318..19205)
                     /locus_tag="PC429_002132"
     CDS             complement(18318..19205)
                     /locus_tag="PC429_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847636.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            complement(19219..20241)
                     /locus_tag="PC429_002133"
     CDS             complement(19219..20241)
                     /locus_tag="PC429_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847637.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALN
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
BASE COUNT         7030 a         3233 c         2174 g         7869 t
ORIGIN      
        1 agacagtttt ttataaatat ctttaaaaag ttaatcaata taaatattaa gccctcttta
       61 atttatctaa atagtaaaca gcatttaaag ctgctatttg cccttgacca gctgctttta
      121 tataagaata tggttttcca acacaatctc cagcagcaaa gcatccatct atactagtct
      181 tcatattatt gtcaacttta atatgaatgc catcaatctc tattccagga accaatgcac
      241 ttggagctgt actatctttt ataacgaata ctccatcaac ttcaatgtgg ttttctttaa
      301 aagatacttt gtttacaagt ttatctccat ctatctgtac tggtctgtca tgtataactt
      361 caatagaatc atctaggtta tctgaacttc tcataagatt atcttttttg tacataggta
      421 taaaataagt ctttgaagtg agttcactta aaaaatttgc ttcttcttta gattcttcat
      481 tatatcctat aactgctacc tttttatttc tataaagtgg tgcatcacat gtagcacagt
      541 agcccacacc tcttcctaga aattcttctt cacccttgat tggtctagta tactctaccc
      601 cagttgcaag gattacagta gtagcttcat acatatcatt tccagacatt attgtaaagt
      661 attctcccat agcataaata ttatttattc ttttattttc aattgaaata tccatactat
      721 ctatatgtga tttaaacttg tcttttaatt catcaccact tatatcataa aaacctaaat
      781 aattatctat tgaaggagct ttaacaagct tattacttaa attatcatta ccaaacatga
      841 taattgtttt atttcttatt tttgcattta tagcagcaga caatccagct ggaccactgc
      901 cgattatagc tatatcatat ctcataatac tcacccattt tttataaata tgattttact
      961 tgcatttcaa ttttgttctt tggcatgaat ccaattaaag tttctactgg ttttccatct
     1021 ttaaatataa tcatagttgg tactgtagat acattgaatt gttgagctat ttctaagctc
     1081 tcatctatat caacttttac aaatctagct ttatccttca tctcttcacc taattctgca
     1141 aatacaggtc ccaacatgtt acaaggtcca caccaagttg caaagaaatc aactacaact
     1201 actcccttac taccttcaac actacttcta aattcagatg tatttataac ttttgccatt
     1261 tttaaatcct cctattaaac tttattatcc ctatataatt atactatgaa atttataaat
     1321 ataaatttac ttttataagg tctttttata ttttatacta attagtatac ccattcttca
     1381 tttctaaaaa caatttttta tatttttatt attttaactt tttgggtatt gacaattcaa
     1441 atgtagttcc aatatctaca tcagaagtta catttatatc aatagataag tttaaactaa
     1501 tcatgcgagc tattgaaagt cctattccag aaccttctaa atctttattt cttacatttt
     1561 cacttctaaa aaatctatca aatataagag gtatctcttc tttttttatg cctataccag
     1621 tgtcacttat aagtaaagtt acctcatcct ctatatcttc tttcaattct aatgctatct
     1681 catcacccaa ttttgtatac ttaaaggcat tatcaatgaa aattaatata agctgtctta
     1741 atttattttt atctgttatt ataactttat tcttaagttt agaattaaac acgaatcttt
     1801 tttcctgaaa ctcagcaatg tcaatatagt cttctgatat ttcttccaat aacttttcta
     1861 aatctatttc ttctaaatta actttaacaa tagagtcctc tttagtaaga gacaataaat
     1921 cagttatcat ttttttaagt cttctagttt ccttcattgc agtagctatt gtttcaactt
     1981 catcatttac tgtactttct ggggatttta acatactttc taattttgat gaaactattg
     2041 ttattggtgt tcttaattca tgagaagcat cttgtataaa cttagcttga tttttccatg
     2101 ctgtttctat tggaattaaa gcttttctag tgagatatac agccacaaag taagtaatta
     2161 ttacagaaat taatattcca attataagaa cagatgtaag ctgtcttaaa gaattcatct
     2221 cagaatctat atttctaatt atctgtattt ggtatctacc tacatctaca tttaattctc
     2281 taaaagtata tccattttct gtgtatttaa aaaacgaatt ttttttatct agtcttctat
     2341 ctggaagtaa ctcatcaaaa tattcatttt gagtatagta acttatcctt ccatcttcat
     2401 acacatacac catatcttta ggatctttca gtcttattgg gttcaaaaat gaagttcttt
     2461 taaattgaga gactatatat tcaagttcat catttatctc actatcaatt ccactatatg
     2521 taagtcccct aaaatatgag tatataaata tagaaaagat tattaaaaaa ctacctacta
     2581 caataatata catttttatt aaatgatttt tagtactggt aaatacattc ttatttatca
     2641 tcgaatatat atcctacctt acgtttagtt ttaatatatt tatcatatcc atatttactt
     2701 agtttttttc ttagattact tacatatact tccactattt caatagtcgc atctgaatca
     2761 attccccata ttctatcata aatctgctct tttaataaaa ttgaaccttt atttaataca
     2821 aaatactcaa gcaagttaaa ctgtttgttt tgaagtttta tttcttcttt ttcaatatac
     2881 actctttttt ctagtgtatc taaatataaa gatttaaatt ctaatccgtt tctttctttt
     2941 atctttccat tagtccttaa tatagcgtat accctagcta ctaactcttc catataaaaa
     3001 ggttttgtca aataatcatt agctcctata gtaaatgctt ctactttgtc atctaatgtt
     3061 tcttttgctg tcaatataag tacaggtgta tctatattat taactctcat tttctttagg
     3121 atatcaaatc catttatatt aggaagcatt aagtctaaaa tgactaaatc atatatattt
     3181 tgatttacta agtataaagc ttcttctcca tcttcacact tttcagtttc aaaatgcttg
     3241 ctaagttctt ctactacact ttctaaaagt atcttattat cttcaactat taaaaccttc
     3301 ataagtatcc cctaccttta tttgtctatc ttaactttat gagattttaa gtcaatatcc
     3361 acattggctt tttcttttat atcatcatac gtatatccca ctatttcaac actttttgcc
     3421 tttgttgaac acaatatatc tatatctgtc tcttctgaag gctttaaaat cttatatgat
     3481 ggcacacgat ttactgttaa atattctcca ttttcattaa cttctgctgt cttaagaata
     3541 atatttccta aatcttttga tgatgaattt tttactttta cttgatacgt aattccctca
     3601 tttgattcat ttactttttt aagttctgat gtagatagca cctcatactg actactattt
     3661 tctagcttaa tattattatt tcttatatta atagtatttt cttttaaatt aacatacaca
     3721 atcttaccct ctgcctcata ttcatatgca taaacttcta caccctttac aaaagtttta
     3781 tgcccacatt ctatgtaagc aactttccca ggcattaaag tcatgtccaa caaagcctta
     3841 ctatccgaag atataatttt tttattctca tcatattcat tatatatcaa ttcaacattg
     3901 gatatattaa atgttgaatt attttctacc tttgttgatg tatctacttt atcaccttca
     3961 tctatattta taatttctga ttttcctata ttcaatactt ttgcatttcc ctcatctatt
     4021 tttatctcat ttttatcttc tgtttgaata tcaactgaat caggctgttc atctttttca
     4081 catgctgtca acataaaaat aaatattgtt aatataatca ttagagaaat tgattttttt
     4141 actattttat tcatattaag cacctcatat agtagtattt tatagtagat ttttatactt
     4201 tttcataaat tctttcaatg cctgtgtaag tatttgatgt ttttcaaaat tcgaattact
     4261 acataatctt tcaaaatcat tccatatttc tttatctata cgtattcttg ttgtttttaa
     4321 ttcatctatg tgttcatcat ctatatgtac atctttttta ttagatactt ttttaggctt
     4381 tatattgtaa tcttttactt ctaaatacca atcatatact tcacaaagct tatctaaatc
     4441 ttcttgagtt atatttattt ttctatcttt cttaggctta atattttgtt ttggctttgc
     4501 attttttaaa cctgtatcat taaactcaat ttgttcaaca cttttactta aagccttttc
     4561 attagaaatc ttttttttgg tgcttacttt ttttctatta cttatagtat tctttttact
     4621 tttttcatac tccttagctt tctcttcttc caagtttcta ttatccaaac ttttattata
     4681 atttaattcg tctttaaata tgtatttacc tttttctgac ttatatcctt ttttatttaa
     4741 aaaagttctt aaagtacttt gactaatctt tagattttga gctatctctg caaatttaaa
     4801 tcctttgctt tgaagttcta aaaatttgtc aattctgtct tcattgttat tcattaatat
     4861 tcctccgtca acatcttctt gtctttcttt tcatacttct tattatattc ttcttttcta
     4921 aatctactct atttgattca attatttttt gtagcatttt attataagtg aaatcatcta
     4981 aattattatt ttttatatag agaaaagttt cctcttcaca tttaataaaa caaattgata
     5041 gcaaccatgc catacccatt ttaacataat attcttcact ttgtatatta ttacagtatt
     5101 caaatatttc tttaatatgt ttatcatcaa cataatatat taaaaacata actaatgcaa
     5161 atctaatttc ccatggattc tttgaataaa catatttttt aagtatatca tacatatttt
     5221 ctttatattt tttcatagac tttaaatcag aacaaaatcc atcgcaaact gaccaattat
     5281 cgattttagg tataaatctt tttacatagt ataaagtttc ttcaaaatta ctatttatat
     5341 tgccaattac cataccttgt agcctaactt cttcatagta tgtatcctct gctacttcta
     5401 aaaattcctt ccaattccct tggcttattt cttttgataa ctttcttaaa ttaggaactc
     5461 taatacctat tatattttct gttccaggta taagcttttt attaaaatct ctatatttta
     5521 tatcttgtat agacatcata tattctaaaa gttcattata atccaattta ttccatttta
     5581 ccctatcaaa catcatttaa ttgctcctta attccttttt tcttattgta acataaaact
     5641 ctacaaaaaa agcttctaac tattagaagc tttttcaaaa acataagaaa cttttcaatt
     5701 ttaaatttaa actaaattaa tgctaatctc ctacaccaaa atctgtatta ttgtataaaa
     5761 cttctatatt tggattttca tccaagaatt tatacttact aacaaaccat ttcactaact
     5821 cttcatctct ttttatacta gaagaattat ttataaaaac attcactgtc gctttttcaa
     5881 aagtatttag gataatctct atatctttat ctatcatctc ttttgtttgt ccttttattc
     5941 caaccataat acaaggtgaa tcaaaatatt cttttacatc ttgtggagtc ttaaaatttg
     6001 catttttatt taaaatatta tttctaaaat cattgtcaaa ggtttcaact ccaattttaa
     6061 ataccacagg aatttcaaaa tactctctca tttccttaag cctatttttg taagaccaat
     6121 gactttctaa aaataatttt tttatatttt tctcttttat aatacacttt attttttcta
     6181 aagtatcctt aggtaattca aaacagcttc cagaatttat gacttcaaga actccatatt
     6241 tcccagttac gttttttaaa acctttagat ttaatttatt catctcttct tctaaattag
     6301 aattatcatc tatataatca caaaaagaac actttcccca tatacaagga aatcctttaa
     6361 gcagaacaat ttctctttga tttttatttg ttatctcact atatctatcc attttctatc
     6421 tattttcctc cagtcaatga tacctttaaa gtttttggaa ctgcatttaa tcctaaattc
     6481 actaaaacaa ctgctaacag cttatctcca taatcagtaa atacttgggt tacaaacaca
     6541 ctaaaaacat cccccagtcc taacactttc aagatttgca ctatatatga tgaacctgaa
     6601 gaggtcatac ctccaaataa aaatgctgaa attattgaac ttattataga tgttggaatg
     6661 gcaaataaaa gtactcctag tggagttttt aaacccctta aaaaattctt tttaaatgct
     6721 attccagcaa gtaatccggt tgatatttga actggtgcaa aatagagaga atacacatca
     6781 aacgtaattc cacttattaa acttccaaac acacctgtaa caacagcgta tttaggtcct
     6841 aaaagtgaag ctatcattat agttcctatt gaatctaagt atacaggtaa tcttaatccc
     6901 atagctataa aagcgcctat tatatttata gaaataccaa aagctataag tgtaatagtt
     6961 tttacacata tttttctttt cacactatat cactccttcc acagagtcta taatgtcttc
     7021 ataaccttta aaaagcctct ttaaaaacat gtacataaat ttcttctcat caacttcatt
     7081 tagaacaatt gcattaggat tttttttata gaaattgaaa gaatccacta tagactgacc
     7141 catagctatt ccatcttcta caacttctac atatgattca aatcctttac atatacttct
     7201 atctataaag tacgctactg ctagagggtc atttatcaca cagccaatta ttccctcctg
     7261 ttcccaatgg aaatcaatat aaaatctagt tatttcagtt atatactttg ccatcttctt
     7321 atcaagtcta tttataaact caataatatt aggagtaagt acaatttttc tagttacatc
     7381 taaacctacc atgtggattt ttttagataa attcttgtaa acataatctg ctccatgtgg
     7441 gtctacccaa taattaaact ctgctactgg agagcaattt ccatgaatcc taaatgctcc
     7501 tcccatagat acaaactcat cgagattttc aaatgctttc ttatctttaa taagtgcttt
     7561 agctatattt gtaagtggtg caagtgctat tattgatact ttttcatgat tatataaagt
     7621 atttattata aaatccactg ctccattttt tgctttagct cctacaacct tttgataaaa
     7681 gttttctcca ataccatctt ctccatgtgt atcttgagct gttacaagtt ttctttttag
     7741 tggtgcttct tctcctatat atactggaat atttagtgaa gaacacattt gaagtgtttt
     7801 tagtgcattt tctgcaccta tatttgctgg aacatttcca caacatgtgg taattccaat
     7861 tacttctagc tctggtgagt ttaaagccag aagaattgcc aaagaatcat caattcctgg
     7921 gtcacaatca attattactt ttctcttttc cattttttat cctccttgat ttgtggtcga
     7981 ggagaactac acatcttttt tccactttca aaaaggtgtg tctaagggta ttaattatat
     8041 actgccacta tataattcct tagtttttta tacttggagt tctcgaacaa gtacagatgt
     8101 aaaatcatat ttttaggcta cttactatag actcttaaaa actatagact gttaaaaaat
     8161 ttttaaacat ctgatttaat agctataaaa taaattttat agcataaaag aatttattat
     8221 attttaatgt atcttattaa agtttattta gaattatgaa ccaactttaa taagatgtgg
     8281 cgcatttaaa ccgattatta ttatacaata atatcttaaa tattacaatt aataacatta
     8341 atttattatc cataaaaata tattaactta tattatttaa taatttattg acagtattaa
     8401 aattaaatta tacaataaca gtgtaaatta acttaacacc gttaagcaaa gaggtgatta
     8461 tatgagtatc aaagaaagac gtaaaaatga aaaagaagaa atgaagaaaa agattatgga
     8521 tgctagcatc gaaattataa accaacatgg atatgaaaat ttatcaataa gaaaaattgc
     8581 tacaaaaata gaatattctc caactacaat ttatctttac tacaaagaca aagcagagat
     8641 tatcagcgat atgaccaata aactttataa cacagtagaa agtaatgcca ttgacattat
     8701 gaataattgc tcatcactac caatagataa acaaatcata gaaattatga ctagctttat
     8761 aaaaactctt tctagtgaac cagagatggc aaaatctatc atgtatagta gaatgaatat
     8821 tatatttgca tctgaaaata caaacaatac gcctagcaat aatggtataa aaatgcttga
     8881 taaattcctt tccattggaa ttgaacaagg aatattcaaa aaaaatatag ataaaagctc
     8941 atggatgctt ataagtgcat tgttaggatt cgttttatgt gttgttgaaa ataaactata
     9001 ttctttacat aatttttctc aacttataga aaatttctta gatattttag tgggaggact
     9061 ttacaatgaa aattcgcaac aaggctacta ataaaaagaa aaaatatgta aaaatatcta
     9121 ttgctctatt ctttgtattt atatgcatta ttattgtaat tgtaagcaac ttaaagccag
     9181 aaaacttggt agtaaaagat atagatatta ataatgttaa aaatggcatt tatactggaa
     9241 gtgctgataa taacttggta aaagctactg tgtctgttga agttaataat ggtaaaatcc
     9301 aaaatattaa tatcttaaaa cacgaccatc ttttaggtaa acctgctgaa aaaattacaa
     9361 caagtattat taaacaacag tctttagatg tggatgctat aacttctgca acttatagca
     9421 gtaatactat acgaaaagct gtagaaaatg ctttaagaaa gggagaataa ttatgaatac
     9481 tattattata tattcaagta aatatggttg tacaaaagac tgtgcaaata tactaaaaaa
     9541 taaactatca gataatgtca ctattgttga tattaataat aataataaaa ttgagttatc
     9601 aaaatttgat aaaattatta ttggaagttc tatatatgtt ggttcagttt caaaaaaaat
     9661 acaagtgcta tgtaatgata atgtagagtt attaaacaaa aaacaagttg gcattttcct
     9721 ttgctgtggc ttttctgaac aagcagataa atatttaaaa tcaaattttc catcatcctt
     9781 attagaaagt gcaaatgcta ttggaatttt tggtagtgaa gctcgattag aaaaaatgaa
     9841 atttttagat aaacttatta tgaaggctgt ttcaaaaggt aattatgaca gttttagaat
     9901 atcacaggat aatatagata attttttaat aaatcttaat agctaaaata aaactaatta
     9961 tttctatgat aaattctttt atttataagt aatattgctc aaaatagtct ttaaacttta
    10021 tcaaaatttt atttttctta gtgaaatcta atgaaactag cacttaatat atgctttatt
    10081 aatcataaca aatatgtttt attaatcata acaaaaagag agtgtctaat gaactaaaaa
    10141 attcattaga cactctcttt ttgtatgaaa tcaaatatat ttttatcatt tcaaaagtga
    10201 aaaatacact tgtctctatg tttggcacat tctcttttta taatataata tctaatttaa
    10261 aacctatttc aaatattgac atatacttaa actatagact tacaaaatca attcatatta
    10321 gcttaaaata taatctttgt tattcaaatt cctatacaca accattattt actttgatac
    10381 tttaaaacat cttccctatt ttttattaaa ttttataata atagtcctat taacgcaaat
    10441 actataaaaa gagtttaact ttatccatta aataaattta aattaaaaca tatagaaatg
    10501 gaaggagaac aatttatgat aaaactaatc ggtatattga tagttgtaat tggatttata
    10561 cttaaaattg acacattatt tactgtatta ttagcaggag tggcaacagg aattgttgca
    10621 ggattagatt ttaatcaaat ttttacaatt ttaggagatt cttttgtctc aaacagaggt
    10681 gtatcattat tcatattaac attgccagta atcggtgtat tagaaagata tggtttaaaa
    10741 caaagagctg tttctttaat agaaaaatta aaaagactta caacaggaaa agtacttact
    10801 atatacatga tagctagaca aatagctggt gctctatcaa taagaatgag tggacatcct
    10861 caatttgtaa gaccacttgt taatcctatg gcacaagctg ctggtttaag taatagtgat
    10921 gaactaaaag aatcggatga agaagccata aaggcattat ctgctgcatc agaaaactat
    10981 ggaaattttt atggtcaaaa cttatttgca ggtagtagtg gagtattact tatagcatct
    11041 actctgactc aatttggata caatgtaact ggacttaata tcgtaaaggc tagtactatt
    11101 atggcagtaa tagcacttgt agtggctaca atccagttta ccttatatga taaaaaatta
    11161 aacaaaagtc ataaaaaata gttttattta ggaggaataa aaatgcaaaa ttttataaac
    11221 atatcattag aaatctttta cgcactaatg ggatttttaa tgattgttat agcatataag
    11281 tcttttacta ccataaataa caataagaaa tatggtacat cattattttg gatacttata
    11341 tcactgccat tcatatttgg tagattgatt ccagccaata taataggaat aatattaata
    11401 ttatctagtt tattaacact ttctaaacaa gttgtatttg caaagtatga agaaccagat
    11461 gaaaattttg gaaaagaaca agcagacaaa cttaaaaata aaatatttat accatcatta
    11521 atattagcat ttgctgcagt agtagtagct atgagccttt ctaattttga taattcttct
    11581 caatttgcaa taggtgtggg ttcaataatt gcattaatat cagcccttat aataactaag
    11641 gcaaaacctg ctacatcagt tcaagatggc tcaagattac ttcaacaaat gggacctgct
    11701 agtatgttac cacaactttt agttgcattg ggagctttat ttactcaagc cggagtcggt
    11761 gaagttattt caaccatgat atctggtgta gtccctgctg atagcagatt atttggtgtt
    11821 atagcttatg tattaggtat ggttatattt accatgataa tgggaaatgc atttgccgca
    11881 ttttcagtca taacggcagg aattggtatt ccttttgtat tgtctcaagg tggaaatcct
    11941 gctataattg gagcattagc attaacagca ggatattgtg gaactctact tactccaatg
    12001 gctgctaatt ttaatatagt tccagcagcc ttgctagaat gtaaaaatga ttatattgtt
    12061 ataaaatatc aagctccagt tgctttggta ttaattatag cccacatatt agtaatgtac
    12121 tttttaggat tttaatataa ttaggaggta aaataatgaa aattttgtta acaggatttg
    12181 acccatttgg tggagaacca ataaacccag ctcaagaagc agttgaaagg gtcaataata
    12241 atataaacgg tgcagaaatc ataaagataa ctataccaac agttatgaca aaatctgttg
    12301 aagctataga taaagctatt caagagcaca atccagatat agtaatatca gttggacaag
    12361 ctggaggtag atttgacatt acacctgaga gagttgctat aaatatagat gattttagaa
    12421 taaaagataa tgagggtaac caggttatag atactattat aaaagaagat ggtgaacctg
    12481 catatttttc aaaattgcct gtaaaagcta tggtaaaaca tatgaatgaa aataaaattc
    12541 cagctagtgt ttcaaacact gcaggaacat ttgtttgcaa tcatgtgatg tatggaattc
    12601 tttatatgat agacaagaaa tatcctaata taaggggtgg atttatacac atcccatata
    12661 caacttcaca agtaatagat aaaaaaaata caccattcat gtctttagaa gaaatagtaa
    12721 aaggcttaga attagctata gaagcttgta ttatatataa agaagatgta aaagaaattg
    12781 gtggtgaaat ttcataataa attttttagt atttttataa attaaaaata ctaactatta
    12841 atttaaatat agactgtgag tagattattc aaacaatttt actcacagtc tttttatggt
    12901 atctacagat acaaaattaa ggtctattcc tctctcccca ctcacacatt gcatctaaaa
    12961 ttggaatcag agattttccc ctttctgata gactatattc taccttagga ggtatttgag
    13021 gatattcagt acgaataatc aagttatctg cttctaattc ttttaatgat aaactcaaag
    13081 ttttatagga gatagtattt atataacgtt ttaatgcatt atatcttaca acttcaaatt
    13141 ctacaagaca gtaaagaatt atcatcttat atttaccact aatcagtgaa agtgtataac
    13201 taaatcctgt ttcacttaaa tctgtatttg atatacagtc tatactcata tattacactc
    13261 tccttttggt tagtattgaa caaaaatgtg cgtacttgtt tttagaataa tttataataa
    13321 aattatagtg taagaataat tgaaagtcaa ttttataata ggaggtgtat ttactaaaaa
    13381 aaaagttatt ttaaatagga ttctaagatt agacatttct atataaattg ataatattca
    13441 tgaaaatcct attttataaa aaataatatc tcttagaaaa tctattaaat aaaattcatt
    13501 ggaggaaatt tataatgaaa gttttattga taaacggtag cccaaaccaa tatggatgta
    13561 cttatacagc actaaatgag attactaaag tattgagtaa acacgatatt aaaacagaaa
    13621 tactgtattt aggtaaagaa acaatcccag gatgtattag ttgcgcatcc tgttttgaaa
    13681 ctggaaaatg tattcgaaat gataaagtta ataaattgat tgaagatctg gataatattg
    13741 atggtattat tattggttct cctgtttact tttctagtgc aactggtcaa ttaacttctt
    13801 ttcttgaccg tctatttttc atagctggaa gtcgcatggc tacaaaatta ggtgcttcag
    13861 ttgtgtcatg tagacgtggt ggtgcaagtg caacatttga ccaactaaat aaatattttt
    13921 caatcagcaa tatgcctatt gtatcatcac agtattggaa ccaggtacat ggctttactc
    13981 cagaggatgt tatgaaggat gaagaaggat tacagactat gcgaactcta ggagaaaaca
    14041 tggcatggct tcttaagtgt atatcagcag gtaaaaaagc tggaattaaa gaaccacaat
    14101 atgaagaaac aattatgact aattttataa aataaataaa aaatgtacta atattaatta
    14161 aaacaaaaca ctaatttata aataaaataa gcataaatgt aggataaaaa gcactaaaac
    14221 agaggtattg aattttatat caaacctctg ttttattatt acattaataa ccaaaccatg
    14281 tataacatca tatatttcta aataaattat aatattatat tctaatgtta ttgaaattac
    14341 aataactgaa tttctgctca taaatttaaa tattctacaa atctcatcta aataacaatt
    14401 ttaatagacc taaaacaatc ctcatactgt tcattttgta ttaaaaatat cacttacaat
    14461 caaaaaccat ttattaagta tattcctaag atatacaata tatatacatg gattggatag
    14521 tataaataaa ataaatactt catactcttc cctttttctc cattgtaaag taacattaaa
    14581 ggtagagaag caatcatcat ccattggtaa ttttgtaaaa tagaattttt taaactataa
    14641 tctccatttg acattgttgt aaatataaat atacacatta aaacataaca aatactcaaa
    14701 ctgaactttt tacctctaca caaataaaaa ataatcccaa gtataatcca aattggtcca
    14761 ccttctacta aaaatggtac tggtaccaaa atcataaata aagcaataac tctgttagtg
    14821 actttaaaaa gtaaagctac tgtaaatatt cctaaaatta taggaatgat tatcatcaat
    14881 aatcctataa taaaatattt aatttctttc tcttttctaa atttctttat gaactctatt
    14941 gcttgtaaat aaatcgctat taaaaataat gttgaaaaaa tgttatttat aattatagct
    15001 ccttctggat gtgaaaatac attattaatt atttgatttc ctatagccat aataacagac
    15061 caaatataca atctacttat atattttttt ctgttactag tgtgataaaa tccttcaaca
    15121 gccatgaata taaacagtgg tgctgatatt ctgcctaccc aatgaaacca aattggtatt
    15181 tgcattgttt gtggcataaa tgctgctata tggtcaaatg tcataaatat taatgctaaa
    15241 attttaatag taaacccact tatacctttt ttaaaaaaat ccatatcata tctcctaatt
    15301 ttgttttatc tctataataa tttttgttcc tattccaatt tcactactta cataaatttt
    15361 acctttatga atctctatta tttctttaga aatagccata ccaagacctg aacctttatg
    15421 catctcacct gtattagtac ctctataata ttttttaaaa atatgtttca aatcactttt
    15481 gcttattcca attccattat cttttattat aatttctata ttatcttttt ttacaatttc
    15541 tacacttatc aaagttcctt cgctattatg aactattgaa ttaaatataa gattggttat
    15601 agcccttctg aataagattg aatctacata tacttcaatt aaatcttcat tactttcaaa
    15661 ttcaatattc ctatttttat atctattatc atttaaaata tcaattatta tatctctgac
    15721 taaactgact aaatttattt ttttcttgtc cagaactata gtattatttt ttaatcgagt
    15781 agataaattt aaatcatcaa ctaaatcttt tatatactta gacttattat atataatttc
    15841 tgtatactct tgtatttcat cttcttcaaa ctcataatct ttgtcattaa ttatctctgc
    15901 atacccttgt atagatgcta gaggtgtctt tatatcatgt gttatatttg ccagccattc
    15961 ctctctcaac tcttcatttt cttttctctc aacttcattt actcttaaag tatcagctaa
    16021 catatttata tttttaaaaa cttcttcata tatacctctg tccttaagat ataaactata
    16081 atttccatta gccatagtct caatactcca tagtacgctt gaaattggtt ttgtaagttt
    16141 tctactaaat aaatatccta cacctaaagc taccacgcta tcaattacta gtgttataat
    16201 gataacttta tttaaagttt ttttaatgtt attttgagaa taggttaaaa ttattcttga
    16261 tatatctcta ctaggtatac ctactattat gtttaaatgt tttttgttta aatatttttc
    16321 tagtacaaaa ttaacatact ttgtttctat atacttgtaa ttattcacca tttgaaaggg
    16381 tgtatatttt tttggagtat cctttggcac atttactcca taaacttcct ctcctaaatc
    16441 atttaaaacc tgtatccagg aatttgattt ttctaatttt tcagctccaa tcttagatag
    16501 tttaaattct ccatcttttt catataaatc tttttcaaaa gaacgtgcaa attcttcagg
    16561 attatttcca gaatctacaa ctttaaaaaa gctattagtt gaaatcacat acaatataga
    16621 tatgatattt ataattacta ctgatatagc tacaaacaca attgtaaata taaaatttct
    16681 agtaatcttc cacttcatat tctaatcttc ctttactgct aatttatatc caagaccttt
    16741 tattgtcaga atatattcag gttttgaagg attatcctct aatttttccc taatttttct
    16801 tatatgaacc attatagtat tgtcaaatcc aacataatct tctccccaca ctttatccca
    16861 tagtttttct ttacttataa tttgattttg attatgtgca aataaagata ggatttttag
    16921 ttcctttgca aacagtccta tagattttcc atcttttata agttctgccc tactttcatt
    16981 taatatatat gggccaaatg atataatttt ttcttcatta ttatttagat ttttactttc
    17041 gttttgagca tcactaaaat aacttaatct tctcaaatgc gctttaactc taaaagccac
    17101 ttccttaggg ctaaaaggtt ttgtaacata atcatctcct ccaatcgcta gtccaagtaa
    17161 cttatctaat tcttcggttt tagctgataa aaatattatt ggagaattgg aggtttttct
    17221 tatttcttta caaatatcat aaccttcgcc atctggtaac ataatatcca atacaatcaa
    17281 atcaggatta atacttttaa ataattctaa tccttctttt ttagtttttg cagtatatat
    17341 attattaagg ccttcttttt ttaggacagt ttctaaaagc ttcaatattt ctacttcatc
    17401 atctattatt aaaacacgat tttccataaa caacctcctg taataattta catatatatt
    17461 agcatatatt ttatctaaaa ttcatttatc ttatttttaa ttaaactaaa atataaataa
    17521 aattactatt atttttagtt aaaacctttt gcttgatgta aaattttcat atgttatttt
    17581 agttaacact atcattaata taattgagat tactatagaa aaaactatca aaatacttgg
    17641 tacattatac ttaaccattt gttggctcat cataatccaa tttggagtca ctgacataaa
    17701 ttttatagct ttatctggta taaaacctac actaaagtat ggaattgcca ttatcaatcc
    17761 acctagtata aatggaatta cagccgattt aaatatagat gaaagtgtta agcctatttg
    17821 tgcaaatata aaagaagcaa aaatagtagt caatataaca attatataca tttgtattac
    17881 tgtaagattt aaattactat ttccaaagta ccataagtta tgaatcgatt catttaaatt
    17941 tttaaaatca aaaaaactaa aacctctaat tcctactaac aatataaaag aaatattaat
    18001 aatagataaa attaatccat tagctactag ttttgataat actactgatt tttttccctt
    18061 tttagttgta tttattaaac ctgtcatatt agaatcatat tctttaatgt aatttccaga
    18121 agttattata aacattatca aacctattag aggtattgag ccttctatct tgtaaaatat
    18181 atctgcataa gtatctaaaa catcaaactt tctaatcaga aaaaatccaa aaaatattaa
    18241 aaacagccaa attatccaaa cactctttga ttttaatatt tttatcattt cagctttaac
    18301 taaatacatc atttctacta cacctccctt gcatcttcta aatcaaaata aaacatgtat
    18361 acatcttcga attttgcttg aactggttgt atattatcct cgcttggaat atcctctgaa
    18421 acaattctaa gtactattga attaatatca ctttgaatac ttacaacctt atatttagat
    18481 tgtatatcat caacttcaca ttcattttta acttgaacat tatatacttt attttccatg
    18541 tgacttaata tgtctttgtg tgttcctttc attaggagtt tgccatcttt aatcattatt
    18601 gtttcctttg caactgattc tatatcggaa attatatgag tagaaagtat tattatctta
    18661 tctctagaca tttttgctat taaattccta aatctagctc tttcttgtgg gtcaagacct
    18721 gctgttggct catcaaggac tattatctta gggtcgttta gtaaaacttg tgctatccca
    18781 gctctcctct tcatacctcc tgaaaatttt cctatagact tatttctcac atcataaaga
    18841 cctactaaat ctaataattc tcctatctta ctatttaaat attttcccct taaacctttt
    18901 aaagctccaa tatactgtaa aaattctttt gctgtaaaat ttttataaac tccaaattct
    18961 tgtggcaaat atcctaaaat atttctataa tcagcatcca ttttaaatat atcctcacca
    19021 ttataaagta tctgaccttc atcaggtttt atcaacgttg ttatttgttt cattaatgta
    19081 gattttcctg ctccatttgg tcctagaaga ccataaactc cattttctag agttactgta
    19141 atcttgtcgt ttgctacttt tttaccatat atctttgaaa gattttctat ttctaattta
    19201 ttcatcttaa ctcccctttt atctacttat ataatctgat tttattacta aaaaacataa
    19261 tgacatagca cctatcaata ttgccatcaa aagtcctatt gctatattga caatgtctat
    19321 attcacttta aatatcataa tatcacctag ataatttcct attattttat taaaaccaaa
    19381 tatcaaatca taataattta catttgatac aactgaaagt aattttttag gtaatatatg
    19441 gattaccgac attatcttag ttatagatat aataaatata aaaaaactta ttgaagatat
    19501 tgaagactta cttataaaag acccaaatat ggctatcatg gcccatcctt caaacatcaa
    19561 aagctctatg cctatattta atattacata ctttgttata cttaaattag ttttcaatgc
    19621 aactacattg tctactattc taaaagattc taagtctcca ttaacaggta ctccttgttg
    19681 catacaagtt ataataaata ttcccattaa ataaacagaa taaattacta atggaactaa
    19741 taaagcaaca aatattttag aatttaatgc ttttttcttc tcttttgatg atagtatgat
    19801 atcttttacc gaagtaacga cttcatctac gtaaatatta gatataatta tagatataat
    19861 gattaacatt cctacattta taagtgaatt tgtaactcta tagttaaata ctttccaaaa
    19921 actaatatcc ttatattcat tcacacttag attatcaagt tttttctttg acattaattc
    19981 aattttttta gaaaattcac ctgtatctgt ttgatttgaa aactgcttta aaacattaat
    20041 cttattttga aatttctcat tacctatgtc gataccactt ctcgtatctt ctatacgtcc
    20101 tttttcttta tctatataat aactttctgt ttctaaaaca ggcgtaataa agatactaat
    20161 cattagtatt aacactaata tgcctagaga tataaacata ctcttagatt tagccaattt
    20221 ttttatttcc caatttacca atatatttct cctcctaaat cattttaatt acacatatat
    20281 attaaaactt aaatcttata taaata
//
LOCUS       DAMKMI010000065        20251 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMKMI010000065 DAMKMI010000000
VERSION     DAMKMI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20251
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.066"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(464..2068)
                     /locus_tag="PC429_002134"
     CDS             complement(464..2068)
                     /locus_tag="PC429_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3847638.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVVDNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            2454..3380
                     /locus_tag="PC429_002135"
     CDS             2454..3380
                     /locus_tag="PC429_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF3847639.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            complement(3500..4498)
                     /locus_tag="PC429_002136"
     CDS             complement(3500..4498)
                     /locus_tag="PC429_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF3847640.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            4602..5039
                     /locus_tag="PC429_002137"
     CDS             4602..5039
                     /locus_tag="PC429_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3847641.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            5125..5712
                     /locus_tag="PC429_002138"
     CDS             5125..5712
                     /locus_tag="PC429_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF3847642.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(6012..6632)
                     /locus_tag="PC429_002139"
     CDS             complement(6012..6632)
                     /locus_tag="PC429_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF3847643.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEVDLTKEINDISIKLS"
     gene            complement(6640..7407)
                     /locus_tag="PC429_002140"
     CDS             complement(6640..7407)
                     /locus_tag="PC429_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF3847644.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGVIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            complement(7448..8269)
                     /locus_tag="PC429_002141"
     CDS             complement(7448..8269)
                     /locus_tag="PC429_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847645.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            complement(8628..10538)
                     /gene="phnW"
                     /locus_tag="PC429_002142"
     CDS             complement(8628..10538)
                     /gene="phnW"
                     /locus_tag="PC429_002142"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF3847646.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPTEDDVNELYAEFETMLFETLE
                     DYTTPIPHVVETIEKLRENGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWDTMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            complement(10817..11602)
                     /locus_tag="PC429_002143"
     CDS             complement(10817..11602)
                     /locus_tag="PC429_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF3847647.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKHSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            complement(11819..12049)
                     /gene="dltC"
                     /locus_tag="PC429_002144"
     CDS             complement(11819..12049)
                     /gene="dltC"
                     /locus_tag="PC429_002144"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF3847648.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            complement(12068..13216)
                     /gene="dltB"
                     /locus_tag="PC429_002145"
     CDS             complement(12068..13216)
                     /gene="dltB"
                     /locus_tag="PC429_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF3847649.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            complement(13216..14730)
                     /locus_tag="PC429_002146"
     CDS             complement(13216..14730)
                     /locus_tag="PC429_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF3847650.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            complement(14851..16041)
                     /gene="dltD"
                     /locus_tag="PC429_002147"
     CDS             complement(14851..16041)
                     /gene="dltD"
                     /locus_tag="PC429_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF3847651.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            complement(16329..17348)
                     /locus_tag="PC429_002148"
     CDS             complement(16329..17348)
                     /locus_tag="PC429_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF3847652.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            complement(17524..18063)
                     /locus_tag="PC429_002149"
     CDS             complement(17524..18063)
                     /locus_tag="PC429_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF3847653.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYRLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            complement(18164..18844)
                     /locus_tag="PC429_002150"
     CDS             complement(18164..18844)
                     /locus_tag="PC429_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF3847654.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            complement(18873..20162)
                     /locus_tag="PC429_002151"
     CDS             complement(18873..20162)
                     /locus_tag="PC429_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF3847655.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
BASE COUNT         6668 a         3493 c         2093 g         7997 t
ORIGIN      
        1 ttttttaaat tagaattatt ttgatgaatg attcaagtaa taggtatgaa attgaatctt
       61 ttagaactgc ttacaaaaaa tattcaaaga aaaatagtaa ttttctgaga ttcacaatat
      121 attttatatg aaatgttata aggtaatcta tttaataaat ttaaatttgc tattatagat
      181 ttagttccaa agtcttactg caaagtaata tacatagttt tttaataaac taataagtgc
      241 aattaataac tacttaattg tttaatatag tacatagata actttaataa atcgatagtt
      301 atttaaaaag gtagaatcaa taaaattata cttataaatc ttcaatacaa taattaaaat
      361 ttaataaaaa tataattgtt gatttaactg taatcttatt taattctacc taacttatta
      421 tataaaacta ttttttatag attataaaat atctaacttt catctacttc aaagaataca
      481 atacttctcc ttctcttata gtctgcaata ctttaatatc ttttattttc atagggtcga
      541 ttgtaagtgg gttttcatct aatattatca aattagctaa tttcccttct ttaattgaac
      601 cttttttatc ttcttcaaaa tattgatatg ctgcatttat agtaacaccc tttaaagcat
      661 ctaatgggct tatcttttca ttttctccta tttgtatacc tttttttgta attctattga
      721 cagcacacca tactgtttcc agcatattag gtgctattac tggtgtatcc tgatgaagag
      781 tgtaaattaa tcccttttct atagttgttt taagtgggct aatcttaaac gctctatctt
      841 ctccaaggtt tttgatatga atatcacccc aataatatgt gtgagctaca aagtatgaag
      901 gtatcatatt tattactttc atatcatcaa tctggtctgc tcttactgtt tgagcatgta
      961 tcataactgg tcttatatta ttttcactac tttgctcttt taagtttaaa actttctcaa
     1021 acgcatctat caactggtct gctgctgcat ctccattaca atgagttaag agttgcatct
     1081 tttcttttag tgaaatatct ataaactttt ctacttcttc atctttgtat ataggatatc
     1141 cacaataacc atcatctgaa ttttcatatg gtctactcaa ccatgctgtc ttaccttgag
     1201 gagaaccatc taagaaaatc ttataacctc ctattttaaa tctatttata tattttttta
     1261 taaactccct attattatct actacagact tacttttttt caaatttaca tatcctacaa
     1321 catccatttt taatttattt tgatttgcca ttgcttttaa aatgttaaat tgattttctt
     1381 ccattaaacc ttcttgagct gttgtaatac catagcttag atatatgttc tgtgcttttt
     1441 caattgaatt aaaaatctca tttgatgatg gttgttttat tttagatgct acattaaaaa
     1501 atgcattttc ttctaaatat ccatttggtt cttcactgcc ttctactcta cctatatgtc
     1561 caccttctgg gtcacgtgtt tcattggtaa caccaagctg ttttaacccc aatgtatttg
     1621 caactcccat atgaccagaa gcatgtgaaa ttagtattgg attttctgaa gatgcactat
     1681 cgagtacact cttatctgga tgcttatttt cttctaaaaa attattatca tatgaaaatc
     1741 caattatcca gtctcctttt tttatattat tcgactcttt gaaatcctgt atttttttta
     1801 ctatgtcttt aaaactggtt gcatcttcta aaggaactag ctttagagta gtagcaaaag
     1861 ctatcaagtg actatgtgaa tctataaaag atggcatcaa tgtcttacct tgtaaatcta
     1921 taatttctgt atctttattt ttcaaagcaa atacttcttc ttttgttcct atctttttta
     1981 ttattttgtc ttttataaga atagcatctc cacatattga atcttccatt gtgatgatat
     2041 ttccattgta ataaattttt gctttcataa aattctcctt acgttgtcta ctttaataaa
     2101 tttttttcat tattttctgc ttaattatat tatcttcttt ataaactttt tctttagagt
     2161 ttaatcatat aaaagtttta tttttaatta ttgtttgcca agaagtaaat actattcatt
     2221 gtaaataaga caaattaata ggatttatac taattgaaaa aatattataa gttgttaaat
     2281 ttaaatatct aaactcaatt atatctgtac aattgtaatt ttttaaatca tgatgtatta
     2341 ttttaaatat acagttacat tattattcac aatataagta tcttagttct caaataacat
     2401 ctcatgtaat ctattcaatg caaaaaattt aaatattttt taggaggttt tttatgagca
     2461 aggaaaataa aaatgtaaga attgctgttg tccaggcgtc accagtcata atggatttag
     2521 aaaaaactgt tgaaaaagca ttaggattaa ttaaagaagc tggtagaaaa ggggcaaata
     2581 tcgtagtttt ccctgaagca ttcatacctg catatccaag aggtctttca tttggatttg
     2641 ttgttggaag tagaacaatg gaaggtagag aagattggaa aagatactat gataattctg
     2701 taccagttcc tagtgctaca acagatttat taggaaaagc agcacaagaa gcaggagtat
     2761 atctatcaat gggtataaca gaaagagatg gtaatgatat aaattgtacc ctttattgca
     2821 caaacttatt tttctctcca gaaggtaagc taattggtaa acacagaaaa cttaaaccta
     2881 caggaacaga aagatgtatt tggggagaag gagatggaag tactcttaca gtagttgata
     2941 ctccatatgg aaaaatgggt tcgttgattt gttgggaaaa ttatatgcca ttagctagaa
     3001 cagctcttta tgctaagggt gtaaaacttt acatagcccc tactgcagat tcaagagaag
     3061 agtggcaagc aactatgaag catattgctt tagaaggaag atgttttgta attggatgca
     3121 atcaatatgt tgaaaaaaat atgtacccaa ctgaccttaa ctactataag gagctagatg
     3181 cagaaccaga aattatgtgt ccaggaggaa gttgtatagt tgaccctttt ggaaaatatg
     3241 tagcaggtcc tattttcaat aaagaagaaa tgctaatagc tgatttagac ctagaaaaaa
     3301 tagtacttag tagactagat tttgattcag aaggtcacta ttcaagacca gatgtatttg
     3361 aattaattgt tcatgaataa tctttttaca caaacatcta cataaatctt tttacataaa
     3421 aaaatgagat actcataagt aaatttcaag tatctcattt ttgcttaaaa cctataatcc
     3481 taattataaa tttattcacc tatatttttt ctaaatcaac tactgttctt ccaacatgtt
     3541 ttcctgccat tattgaatct atactatcat ttaactcttc caatgatact acatttacca
     3601 tatcatctaa atcatctaat ttccattcat ttgataataa atcccatatt tttaatctta
     3661 agttcattgg acaatccaca gaatctatac catataaagt aattcccttt aagataaatg
     3721 gatatactga acttgtaaaa tcaactcctc ctgcattacc acaagttgtt actgctccac
     3781 aataattagt tgtctttatt gctgttgcta gagtatttcc tcctactgta tctattactc
     3841 cagcccatct tggtctaagc agaggtcttc ctgagttatc atccacttct gacctatgta
     3901 taacatcttt aacacctaag tttagtaata cttctttttg ttcttctaat tttcctgttg
     3961 aaactacaac tgaataacct aatttaatca atattttagt tgcaattaca gcaactcctc
     4021 cagtaccacc agtgactaat acatctccca tattcttgtc tacagaatct ataaatttat
     4081 acacagataa agctgctgta aaccctgctg taccataatc cattgcttct tttagtgaaa
     4141 gatttttagg aagtttaact acccaactag aaggaactct tatgtattct gaaaaaccac
     4201 cagaagtatt cattcccaag tcataacctg ttactaaaac ttcttctcca attttaaaat
     4261 catcacaact actagattct actataccag atgcatcaat tcctggtgta tgaggatact
     4321 ttctcgtaac acccttatta ccatttgcag aaagtgcatc cttgtaattt aaagaagaat
     4381 attttacttt tattaaaact tcgccttctg gcaatgtatc cacttctctt tcaactatat
     4441 ttcttctaaa atttccttct tcttctgtaa tatatagtga tttaaatttt ttattcataa
     4501 atttcctccc aatacattta gttttatgtt aaactaatta tagtttataa atagttttat
     4561 gtcaaactaa tttaaataga ttaaagattg gagaataatt tatggaagat aaatatattg
     4621 tacatttcat aagtaaaaca aaggctagta tgattaaatt cattgaaaat aaactcagta
     4681 aaaatggttt aggtgagtta atacctactc atggaaatat tttaactgca ctttatgaaa
     4741 ataatggaat acttacaatg aaagaaattg cagctaaaat aggaaaagat aaatccactg
     4801 ttacagtgtt ggttaataaa cttataaacc taggatattt agaaagacaa aaatgtacaa
     4861 atgacaaacg tatcacgtat ataaagctta caaaaaaggc actgcttata gaagatactt
     4921 ttaatagcat ctcaacacaa gttaaagaga cggcatatca taatattaca gaaaatgaaa
     4981 aacaagaatt tcttagaatt ttaaaaaaaa ttaatgataa ttttaaaaat gctgactaaa
     5041 ttgagcataa ttatatctcc tattggaata ataaataaaa agaatcaaat attgtaaaaa
     5101 tttattgtga tagagaggta gaatatgaat ttaaaagata gtaaaactaa agaaaattta
     5161 ttaagagctt ttgcaggtga gagtcaagct agaaatagat acaatatttc tgcatctgta
     5221 gcaaaaaaag aaggattata tataatagag tctttattta attacactgc tgaccaagaa
     5281 aaatcacatg caaaacaatt cttttctaaa ttaaaagaat ttgctggtga aaatataagt
     5341 atatcaggag cataccctgt agatttatat gacaagacag cacaacacct taaagctgca
     5401 caacataatg agcttcaaga gtgggatgat gtatataaaa actttggaaa aacagcacgt
     5461 gaagaaggat ttgaatctat agctaagtta tttgaaaata tagctgaaat agaaaaagtt
     5521 catggtgata gatttggtag atatgctact gatttagaag aaggtacttt attcagaaga
     5581 aatgaagata tacaatggat atgtacacat tgtggccata tacatatagg taaagaggct
     5641 ccaaaagctt gtcctgtatg tcttcatcca caaggatatt tcttagattt taaaaactca
     5701 ttatttgaat aaactaaggt aatcattgta tttaaacaat ttactatatt tatatcaagt
     5761 atttttataa atactgtatt atcttaataa caataaaatg aaccattgat attactataa
     5821 gtagaaaatg tagtttaaat acaatttata gtagttcatc atggttcatt ttattgttta
     5881 ctaatcccta taacctactt aaatctatct ttaactttta cacactcaca taaaatctca
     5941 tctttaaact aaatatttaa ataaagtgta ttttaagtat attattataa tacattatat
     6001 aagataatac actatgataa ctttatactt atatcattta tttcttttgt caagtcaacc
     6061 tcaataatct taactacatc tttttcaaac tttacttttt tataatccaa acatcctact
     6121 acgaatggaa ctccaaactt tctaaacaaa taaatctttc cactttcatt gtatattgat
     6181 gtaaaatttt cttctattat attcactatc aattttaact cttcaaaagt tattatagat
     6241 ttaactaaaa acttctctat tatgctcaat ataagcagtt cttttttacc tatgctttgc
     6301 tcttttttat ataaattttg aaatatttct ttggtataat tacatataac ttctttactg
     6361 agaataaaat caatgtcaaa ttctttatta atatcctttt tcataaacat atttgcaata
     6421 tcatcaagag aagtctcttc tttcatagac aatattaact ctattctttc tagtatttta
     6481 tctcttggaa agaaagtctc ttgacctgta aaagctgatt tttttataaa ccattcctct
     6541 ggtattatgt tttttctttt ccatctatat aattgaccat aagatatatt agttttgctc
     6601 aataaatctt tcttagatat taattcttcc ataacttttc tagcccacct ttatactgtt
     6661 ttttacagta gaattatctg aaactgataa attatcactg tattcaatat ttccaacgtc
     6721 acaattatct actagagata tgcttttgcc tcgtatatct tttattgatg catcttctaa
     6781 ttcaatagta tctccctcta tcaaattaca tttaaacttt cctgaaaaaa tattaatgat
     6841 tttgattagt gaaaaagcat ctttgcttct tcctattttt atattagttg cccctatctc
     6901 tttacaataa gaacctcctc ctgaataaac atatatattt tctccattta aaagaccatc
     6961 acattttatt actccatata cctttatatc ttcacattct atatttgatt tatttctcat
     7021 ttcgccatag atttttatat cttgtgcata catattttca tttacactta attgtcctct
     7081 tattgtaaca ttattacatt taagtttgcc atccactttc tgttgagcag ttataactaa
     7141 gtcatctatt tctgcatctc cctctatatt aagttgacca ttcactctta cacgctctgc
     7201 tttaagattg cctcttataa tagtttttcc gttaacaaca aaatttttta tattgatgtc
     7261 accttcaaat tttgcatctc caaggacttt tacttcgtta cttttaacat ttccttttga
     7321 tttagaactt cccacgattt ttatattttc atactcacca ctacttataa agccttctcc
     7381 tagtattttt atagaccctt tttccatatt attacctcat tttaaatttt atttttaaac
     7441 ctatttatta cttatccttc tttaatttta ttttttcact tatattcttt atttcaccaa
     7501 ctattgaatc ttcatcaatc tctatacttt cactgtactc caaactttct acataacatc
     7561 ccttgcctat tttaatattt tttcctctta caactttgac ttcactattt tctaagctta
     7621 tttcatctgc ttctataata tttgcaaaca atttactttc tgtatacttt ctaattgata
     7681 atagacctaa acttatcaaa tttgaagaca ctttttcaaa gttgatatgc tttttttcta
     7741 gcatacgaaa ttttttgcaa taatctccat gccccttttt tatatttata ctacttgctc
     7801 caacttcatt aaaattagaa gttccattta aactaatatt aacttcttca caattcaaaa
     7861 atccattaca ctccaagatt ccacttaaat tgatattttc acaatctata ttctcatttg
     7921 cttttaaata tccttctcca tgaataatat cagattttat acttccttct acattaataa
     7981 ttccactagt atcaatttca acagaattaa tatttcctgt acatctaaga agtcccgata
     8041 tctcaacttt tgatgccagt atgtttcctt ctactttact taaaccagat gtaaaaaatt
     8101 cattacattc tgttgtccca aaaacttttg ctgaaccaga agaatcaaat ttactacatt
     8161 ttacacttcc ttctattttg cctgttcctg atatattaac attatcaaaa attccacctt
     8221 tacattttcc aacaccatca aatcttagtg aacttaattc ttcattcata atgatatcct
     8281 ttctttaccg tattttatct ttaaacctaa tataacaaaa cattgttacg ttgtcaagta
     8341 tgataataat acttaataaa gcaactatca tttattttct ataaataggc ataaaaataa
     8401 tagggaaatg ctttatttaa actataaatc taatttctta gattgtggtt taaataaaat
     8461 atatccctat tactcaatct ttatatattc attgtataaa cttctaaaat ataagtcttt
     8521 aaattctact gtcaatactt tttatacact gtttgtgcta tcatctcaca cacctaaagg
     8581 agtgggcttt ccgcactatt ctgtaaccta caagacacta tcttcttcta tctattgata
     8641 aatttttcta ttacatcagc tagtctctcc atatcagttg gataaacttc acctatgcta
     8701 cctattctaa atgtatctat atcagtcaat ttacctggat agataacaaa tccattatct
     8761 tttaaataag tataaaattc cataaattca aactttgcat ttttaggata taagaaagtt
     8821 gttattacag gtgattgagc attatcattc accaaagtat caaagcctaa agattttaat
     8881 cttgaagcta ttgtaaactg attttcttta tatctagcat atcttttttc aacagaacct
     8941 tcttcttcca attctaataa agcttgataa aaagctctta caacatgtgt aggtgaagta
     9001 aatctccatt taccattatt tttctccata gtatcccatt gcgcatacac atcaagagat
     9061 aatgatttag ctataccttt acatttagat aattcctctt tattagctat tataaaacca
     9121 aatccaggta ctccttgtat acatttgtta gaactactaa ctaaaaaatc tatattaaaa
     9181 tcttcaacat caatctcaat tcccccaaaa ctactcatag catcaactat ataaattttg
     9241 ttatattctt ttgcaagttt ccctacctct tgtataggat ttaatctacc tgtagtagtt
     9301 tcacagtgaa ccattgatat atgtgttata tccttgtttt cttttaataa attttctact
     9361 acatttaaat ccactgcttc tatatcttta aatgtgcagt caacaaattc tatgttaaga
     9421 taattacata tatctttcat tctctttcca tatgctccat ttgcaattac aagaagttta
     9481 ccatcttttg aaatagtact tcctattata gcttctactg aaaaagtccc acttccttgc
     9541 attaatacac ttgtgtattt atctgtattt tgggttgcta gagatactaa tcttcttcta
     9601 acatcttgta ctaggttatt atattcaaca tcccaagtac accaatcttt taacatagat
     9661 gctcttactg atttagtagt agatagtggt cctggtgtaa gtaatatgta atcattttct
     9721 ggtacaaaat ctgatttaat agtttcattc tctattttta ttagtatatc ttctaactca
     9781 gccatagttt ctattacaaa atgtgcacct gcctctttaa atttctttga aactatactc
     9841 atcttagctt ttaattcttc cttatccatg ttctctactt cttcttgtgt aagccctaac
     9901 tcacttgaac cttttattac agcaacactc cacattcctg catttacgcc ttcttttaca
     9961 tcacttattg tatctcctac ttttaccata gaactcatag gagacacccc taaagcttca
    10021 gcatttttat agcacatcca aggatacggt cttccttgag atacttctga tggtgttaca
    10081 agaaaatcgg gtgagtatcc tttctttgct gcatttggtt caactatgtt catcatctct
    10141 cttgtataac ccgttgtaga acctattttt aatccatttt ctctcaactt ttcaatagtt
    10201 tcaacaacat gaggaattgg agtcgtataa tcttctaatg tctcaaataa cattgtctca
    10261 aattcagcat aaagttcatt tacatcatct tcagttggca ctttaccaaa cttatctgac
    10321 caaagatttt ttatcctatc catttcacac atttctctta tatggtctat ttttaatttt
    10381 cccataggtt ttcttgcttc ttccattgtt acatctatac ctcttctttt aaatatctct
    10441 ataaatacat taagaggtgc aaaacatcca taatctactg ttgttccagc ccaatcgaat
    10501 actactgctt ttattttttc tccatatatc tttttcatat ttatagcctc catagattct
    10561 ttttagaatt tttgtgtttt taattgtttt tacttttaaa gtataacttt catatgttaa
    10621 ttatacatca tatttaggtt aaatatatat taaatttctg tttttagttg tatttagttg
    10681 tttttatttt gtatcacatt cccataaaat cctaagttac aatctaaaat ttactttcta
    10741 atcttaatct atgaactgac tatattttat gaatttagtt gtattgagca tagttgtatt
    10801 atataaaact aagggtttat tttgcttcta tccatgttat atttatctca tttaacttat
    10861 ccttccattc attcggaggt tctacatcac aaataacaaa atctaattcc tctaaatctg
    10921 ctatcttata catactagca attcctattt tagaactatc acataataca aaactttcct
    10981 ttgaattttt tatatattct tttgacaaca atgccttatt ttggtctata ctactaattc
    11041 caaaactatc aagtatacct tcactagata taaaagcttt atctacgtat aagtttttca
    11101 tagtatctat tgacattgga catgctgtcc tttggtgttt taaatttact tttccaccaa
    11161 taaatataac ttctccatca aacaaacctt gattctcata agaaatcaat tttgatagaa
    11221 ctggataaga gtttgtaata atttttaaat tgtttacaga tattatataa tctacaatct
    11281 gcaaattggt agtaccttca tctatgataa ttgactcatt gtcatttaca aatttagctg
    11341 catatttagc aatactatgc tttttatcta ggttagtatt gtttctcttt atatgttctg
    11401 gttcaatcac tccataattt atttttatag caccaccata tacctttttt aatttatttt
    11461 cattttctaa ttcttccaaa tatcttctta ttgtttctga tgatacttct aatcttttta
    11521 ctaattcttt agtgtttacc ttcccttttg aatctaataa tttaagtatt tctttttttc
    11581 tatctatcga atttaaagac attcattttc ctcctaaata taatatattt ttctcctata
    11641 atattacaat gatacctaag atttatgaaa tcatatttat gtattaaatt ttttacttaa
    11701 ttaaagttat tttcaaaaga ctttgtatta tcattaaaaa agtccatcaa atattagtat
    11761 cagatggact ttgtaaaatt ttattttata aattaatatt taaagtctaa ctactaattt
    11821 attttcttgt ctccaagaat tttactaaat tatttacagt agacatatct tttctctcta
    11881 aatcagttgg ttgaagctct atccctaatc tattttcaat ttcaagtaaa acttcaatta
    11941 tagctaatga gtctaaaagt tccgtctcaa ataaattaag gtctaaatct tctcttatct
    12001 catcagttcc taatacatct tcaaatattt ctataactgt ttcttgcata aattttcctc
    12061 cttaaaatta agctaaataa cctgagaata tcagcaatcc aaaacacaca atatggaatg
    12121 taatgaaaat ctgcactctc tcgaaccatt tatctttttt atgtttttta taaaatttag
    12181 attttcgctg atatatatca gttaacacaa gtgccaatcc ttggtataca ccataagcaa
    12241 cataatacca agttagacca tgccaaaatc ccattgttat catggttatc atctgagcca
    12301 catgagcagc agtagtcctt ttcttaaatc ttttctttct cattgatgac ataacaaatc
    12361 tagaaaaaat ataatctcca aaccatcttg aaagacttat atgccatctt gtccagaact
    12421 ctttcatatc tttgctgata aatggcttgt caaagtttat aggtacttgt attccaaata
    12481 tgtaccctgt acctatagca aataaactat atcccgcaaa atcaaagaac aggtataaac
    12541 tatatgcgta catataactt aaaatattta taaaactcat atctttaggt attcttgata
    12601 cccaatatgt atttatcaaa aatgctataa caaacttata acctactccc atgacaatat
    12661 tttttattcc tggcagtaag tattcttcta tatactcttt tctagagatt tgtttttcca
    12721 agtcctgttc aaatcttctt gacctatcaa taggtccaga acttaaagtt ggaaaaaata
    12781 acataaaata aagcatcttt gaaattttaa cttcttttat agctccatca taaatttcaa
    12841 taaccatttg tatagttctg aaatttaagt atgatattcc aataaatcca attattccaa
    12901 aagaagtaac tggagatatt ttatttatta ttattgggag cattgatgca aacaagaatc
    12961 cccaatatat gtatttattt ttagtttttc ttcttacata ttcatatcct ttaactatta
    13021 ttacttcaag aataataaat attaccaagt actttagttg gacatcaatt cctactatta
    13081 agaatatcat aaacaaactt gccaacatac cataatactt tatattcttg cccattagac
    13141 ctaaaattac tgctggtatt gatgttaata ataatatata aagataaaaa taatctccat
    13201 attgtgaaaa aatcattaaa tttcctccat aagtttcttt ctgtctattt taccattgat
    13261 atttgttgga aactcagaga ttattgaaat atttcttgga atcatatatg aaggtatata
    13321 ctttccaagt tcctttttta ttatcatccc atttttaata ttacttaaat cattcttttc
    13381 atttaactca acaatacctt ttaagtaagc aattttttcg tccttatata ctggtaaaac
    13441 tactgcattt tttacattat gtacttttct caaattattt tctatatctt ctatttcaat
    13501 tctaaatcca tttaatttaa tctgaaaatc ttttcttcca caataatata tatttccatc
    13561 taaaagatat cccatatctc cagttttata agctctccac tttacaccat ctatttcatc
    13621 atagaaaaat acttcatcag tcttttcttt attattgaag taacctttag aaactgatgg
    13681 tccaattatt ataatttcac ccttttcatt ttctttaagc tcatttccat cttcatcaag
    13741 tatttttatt ttacaatttg acatagggta tccaactgga agactttttt catcatctat
    13801 agccttttga gtcatatcat ttacacttac accaacagta gcctcggttg gtccatatcc
    13861 attgataatt cttgtgtttg gaaatctact cataagttct ttagtaagat tttttgaaag
    13921 tgcttctcct atgaatatca ttgactcaag gttaggaagc attttactat taaactcttt
    13981 ttcagttata catactcctg caaaagaagg agttgacacc catacagcta tatctgagat
    14041 actaaattgt ctaaatagtt ctttatagtc tgctaataca tcttttgata ttgaaaataa
    14101 tgtagctcca tggataagac cagggtatat tgttgtaact gaaaggtcaa aagaatatgc
    14161 tggttggttc attattactt tttcacttcc atctatgttt agatatgggc ttatccaatc
    14221 tgaaaagcta tctaaattgt ttgaacttat ttgaacccct tttggctttc ctgtacttcc
    14281 tgaagtaaat agtatatatg cattttcatc atcttttacc cagttttctt tatctaatga
    14341 ttttccctga tattcattta ttatataatt taatttatcc atatcaatta cattgatatc
    14401 tccaaaattt ctttcatcac taaagttaaa taataccttt ggttttatct ctttagtgac
    14461 ttcaaaaact ctatcaattg gaaaactaat atcaagaggc acatacgctc tacctgattt
    14521 taaagctcct atcatacatg ccattatcat gttttcttta tttccataaa ttactatagg
    14581 agtatcttct tctttgtata catcctttag aaatactgat atagcatctg aatattcatt
    14641 taaatcttta taagaaagtt tatctccatt acacattagt gctgttctat ctgtatttga
    14701 atattttttt attccttcaa taattttcat ataagaatgc tcctttaaaa tccgttataa
    14761 ataaatggca attctttaaa tggagtaaat gttagaactc ctatcgccaa tattataacc
    14821 atagttaaac aaatactagt taacaaatct ttattgttct ttaaatattt taaataatct
    14881 ctcacagaca tctacccatc ctttcgtacc aagatgcatt acatccctta aataatactt
    14941 atctgaccct ttatctttaa ggtttagaac ttcaaagcct ttgctttctg ctattttttg
    15001 tgctttatcg tagtactgat ttctttcttt ttcagaaata ccagtcaaat tatagaattt
    15061 atccatactt gggattaata caacaactgg cttaatacct aagtcagtac atacatttag
    15121 catcagttta taattttcaa actccttaga tgttaataaa tttacatctt tgtctcttcc
    15181 tttgtattgc tctataccat ctttaaaatg ctctttataa tagtacttat caatatttag
    15241 tggattttta ccaactcttt ttttagcatc ttcaatggct ttctttctct catatgacca
    15301 atttattggt ctttttcttt ttgttgctct ctttttatct agcttaatta atcgtttata
    15361 aagaataccc ttttctttaa gagctataca gtactttctt ccttgaaaat aaggctctaa
    15421 aagcaccttt tcagcttttt caggaaatgt cttaggctca tatagcttag catataatgc
    15481 ttctgcttta tactcatcac ttccccataa taacttactt gatttttttg catattcaat
    15541 cttattttgt ttagatattt ttggattatc taaaaatcga taaaattgta ttggtgaaaa
    15601 tctagactga taatggtgac ttgtgactcc gtctttttcc ataaaccatt gcatagaaat
    15661 taacaagaca actttcttat tatcaatatt aggattcata cttcctaata ttgcagcatc
    15721 ttgtaatgtc tgagtatagg ctctcccaat tgcaaacacc ttatttttac tcctatttgt
    15781 attaaaatag tactttggat gttgttttgt tgaattacta agttctgatg accccaaaat
    15841 cataatatcc ttttctctta aaaaatgatt atttgctgta acccctttat cttttatatc
    15901 acttaaagta tcatccataa ttggaagtag atttttctcc ctcaaaagtt catcagtttt
    15961 agaatcaaga aatttgtcta acccaaataa aaatactacg cctattataa aaggtgtaat
    16021 aaagtatatc aattttctca tattttctct cctctaaaaa tattcaaatt tatgttatta
    16081 ttaatcatat tagttttgat atgtatattc aactacaaaa ttgtaataaa atcgtcaaaa
    16141 ctttttttga tactgttaca aaattgtaaa aaaaaagaaa aaaacgccaa aaataaattt
    16201 ctttttagca ctttttcata cttataattt gacattttaa cacttggcta agtatacacc
    16261 ttaccttaac ttaaaattaa atttatttaa gaatatatat tataaattct tatattttct
    16321 aatataaact atatactatc agctggataa acaatactac tttgtcttct tatctcatcc
    16381 ataatttcca ttactattat tgaattttca tgagaattta tatttgattc tattttattg
    16441 ttatttataa gacttataaa ctctacaagt tcataataca ttccttctat ttctctaggc
    16501 ttttcctctg ctttttcttt tggaatagat atatcttctt ctcttccatc tctgtattta
    16561 ataatagctt tttcaaatag attaagcttt tcaattatta tacttccctc ttctccttgt
    16621 atttcagatg gtatataaga atcagctact ttagaatact gaattatcgc atccatatca
    16681 tcatattgaa atacagctga accttctcca tccactccag tttgaagtat atgagaaact
    16741 gcctttacag tttttggagc gccaaataaa ttcaccatag gatgtataca atacactcct
    16801 atatccatta aagctccatt tgataactct tttttaaagg cattttctat aattccattt
    16861 ttatatttat catatcttga agaatactga caatagcttc caaaaaatct tcttattttc
    16921 cctattttat ataagttttc tttaactgct ttaaagtttg gaacacatgt aattctcata
    16981 gcttccataa gcaatacatt attatctttg gctgttttta tcattgattt gacttctttt
    17041 aaattagatg ctaacgactt ttcacaaagc acatgttttt tgtgttttaa acaagtaatg
    17101 gtttgctttg aatgtaatgc atttggagaa gctatgtata cagcatctat atccttcgat
    17161 ttagccattt gttctaaatc atcaaaaaaa atacttgctc catatagagt tccaaattct
    17221 ctagcttttt ctaaatttct agaatataca gcaaccaatt caaagtcttt aaccctagat
    17281 gcagctctta gaaatatatt tgctatattg cttgtcccaa caataccaaa tcttatttta
    17341 cccatcataa atcactcccc attttctaca ctttatttta attttaataa aaaactttac
    17401 aacttataga tatattataa aataatttta tcaaataaaa aagaaagcta attttaaatg
    17461 atttcaccat tttaacatag ctttctttta actacaatct aaattcaact tatattttta
    17521 aagttatctt tctactatat ctttatttcc cctcaaaaat tttatagtaa gaatttcatc
    17581 cacaaacaat gttactaaaa gtatagttac aacaattggc caataaggta ttataagtct
    17641 atataagcta tcaactatat acacttgaaa atatctaact ccaataaatg tcaatatagt
    17701 ccaatataca gaaaacttta gacatactat tcctctacta ttgtacttat aatctgaata
    17761 atcccaatag accttattaa aatatacttt cataagtatt cctgtaatat attctactac
    17821 tgtcggcact actagacata ataaaagcaa tgcaaatata ttattggggt caataaatct
    17881 tgagacctct attataatcg acattgcaat tgcatacatt ggtttaaatg gaccatttaa
    17941 aaatccatct tcttgaaaat gcccttgttt ataataacta tataagtttt caattatcca
    18001 acctataaaa ccatacaaga aaaaattaaa taaaaaatat aatagagaac ctacattatc
    18061 catgaatatt cctcctagct gtttatactt attttttctc atattcatgg atttattcta
    18121 aatcaaagaa attatattta atttatttaa ttaatacttt atattataga ttgtgacatt
    18181 ctttagagtt caaacttttt aggtattctc taacttccag taaattttca agtatcttat
    18241 gtgattgtga tttaatttgc tcatctggct cttttaaatc tggtacattt atacatctta
    18301 taccaccatt gtatgcagct tcgactccca taggcgaatc ctctatgaca atacaatttt
    18361 tgggattaac atttatcttt ttagctgctt ttaggaatat ctcagggttt ggtttagagt
    18421 ttacaacatc atctccacaa actatagcat caaagtaatc ttttaaattt gccttagcta
    18481 atctctttac tgctctttct ctctttgttg atgttgcaac agccattttg tatccatttt
    18541 cttttaaaaa agagattagt tcattgactc ctaatttgat aggagctccc ttcctctcca
    18601 taaattcaat catgttttta gttttttcgt catataaatc tattattggt acagaactat
    18661 cgtatatatc tgtaagacct tctattattc ctttacgatt tcttcccata actgatgtat
    18721 aaatttcctt agtcatagta tatccatact tttcaaacgt ctccatccaa aattcaagag
    18781 aaattctctc tgagtcaaac aaaactccat ccatgtcaaa aataattcct tctacttttt
    18841 gcatacaatt cctccaatat tttttgatat aattatatta cactttttat gattaatttt
    18901 acaaccctca atttatccaa ttctaatatt gtaaaattgt aattttcaaa agttacactt
    18961 tggtttactt taatatttga accaagcata taggatatcc acccacctat actatcaaat
    19021 ccatcatgct ctatatcaag tccaaaatac ttattaatat agttgataga ttcagttcca
    19081 tcaactaagt aagaacctgc tcctattttc ttaatatgag tctcttcttt gtcaaattca
    19141 tcttgaattt ctccaactat ctcttctagt atatcttcta ttgttaccac accagatgta
    19201 ccaccatatt catccacaac tatagcaagt tgaagttttt cctttcttat tctctcaaga
    19261 gctttatcta tagttaaatt ttcagatata tatataatat ctctcaatat ttcttctagt
    19321 tcaattttat tttcatttat cttttgattg tataaatctc ttatatgtac aaaacctaat
    19381 atatcatctt tattttttct gcatactgga tatcttgtaa caccttcctc ttttaaaata
    19441 gctaatatct tttcttcaga atcactttca tatatacata ccatatcagt tctaggaacc
    19501 attatctctc taattttctt ttcttcaaat tcaaaaatat tatcaactaa tctttgctct
    19561 gattcatcta taagtccact tttataactt tcttctacta atagtcttat ttcgtcacct
    19621 gtatggggtt catcaacctc atcagcttgt gaatatccaa atggtttaag aaataaatca
    19681 gtacttaagt tgaaaaaata tattattgga tatgttaact tataaaatcc tactaataaa
    19741 aaagatgtac tcaacattat tttttctgta ttataaagtg ctaaagcttt aggaaccaac
    19801 tctcctatta caacctccat cattgttata attaaaaaag aaatggaaat tgatatagta
    19861 tatataatac tttcactaag attaaataca cttactattg gtaaaattaa ctctttaact
    19921 gtagattctc ccatccaacc taatgcaagt gaacaaagtg ttataccaag ttgacatgca
    19981 gaaagatagg aatttaagtt gtcttttatt attttacatc tttttgcatt attattacct
    20041 tcagttatca tggtatcaat cctagaacta cgaagtttta ccatagcaaa ttcacaagct
    20101 acaaaaaaag catttatcaa aacaagcatt actataagtg ttacttttaa taactcccgc
    20161 ataaagtatt ggttcttata atgtaagaaa tctataagaa ctcaaagttc cccccttata
    20221 tttataaatt taatttataa tatatattct a
//
LOCUS       DAMKMI010000066        20250 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMKMI010000066 DAMKMI010000000
VERSION     DAMKMI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.067"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..601
                     /locus_tag="PC429_002152"
     CDS             <1..601
                     /locus_tag="PC429_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3847656.1"
                     /translation="VFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQ
                     RELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKN
                     MIRNRKLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVK
                     DLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            719..1024
                     /locus_tag="PC429_002153"
                     /pseudo
     CDS             719..1024
                     /locus_tag="PC429_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1037..1942
                     /gene="truB"
                     /locus_tag="PC429_002154"
     CDS             1037..1942
                     /gene="truB"
                     /locus_tag="PC429_002154"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF3847657.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2073..3002
                     /locus_tag="PC429_002155"
     CDS             2073..3002
                     /locus_tag="PC429_002155"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF3847658.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3106..3363
                     /gene="rpsO"
                     /locus_tag="PC429_002156"
     CDS             3106..3363
                     /gene="rpsO"
                     /locus_tag="PC429_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF3847659.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3603..4448
                     /locus_tag="PC429_002157"
     CDS             3603..4448
                     /locus_tag="PC429_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF3847660.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4573..6684
                     /gene="pnp"
                     /locus_tag="PC429_002158"
     CDS             4573..6684
                     /gene="pnp"
                     /locus_tag="PC429_002158"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF3847661.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6950..7693
                     /locus_tag="PC429_002159"
     CDS             6950..7693
                     /locus_tag="PC429_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3847662.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7851..9098
                     /locus_tag="PC429_002160"
     CDS             7851..9098
                     /locus_tag="PC429_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF3847663.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9133..9378
                     /locus_tag="PC429_002161"
     CDS             9133..9378
                     /locus_tag="PC429_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF3847664.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGDILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9388..10611
                     /gene="dapG"
                     /locus_tag="PC429_002162"
     CDS             9388..10611
                     /gene="dapG"
                     /locus_tag="PC429_002162"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF3847665.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10724..11458
                     /locus_tag="PC429_002163"
     CDS             10724..11458
                     /locus_tag="PC429_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF3847666.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11581..13992
                     /locus_tag="PC429_002164"
     CDS             11581..13992
                     /locus_tag="PC429_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF3847667.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13995..15053
                     /gene="mnmH"
                     /locus_tag="PC429_002165"
     CDS             13995..15053
                     /gene="mnmH"
                     /locus_tag="PC429_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF3847668.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15047..16381
                     /gene="rimO"
                     /locus_tag="PC429_002166"
     CDS             15047..16381
                     /gene="rimO"
                     /locus_tag="PC429_002166"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF3847669.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16368..16910
                     /gene="pgsA"
                     /locus_tag="PC429_002167"
     CDS             16368..16910
                     /gene="pgsA"
                     /locus_tag="PC429_002167"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF3847670.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17198..18244
                     /gene="recA"
                     /locus_tag="PC429_002168"
     CDS             17198..18244
                     /gene="recA"
                     /locus_tag="PC429_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF3847671.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGISVSKEIVEE"
     gene            18420..19961
                     /gene="rny"
                     /locus_tag="PC429_002169"
     CDS             18420..19961
                     /gene="rny"
                     /locus_tag="PC429_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF3847672.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8297 a         2050 c         3908 g         5995 t
ORIGIN      
        1 agtatttgaa aatactaata atcatatagg tttagattta ggaattaaag aattttgtat
       61 ctcaagttgt ggagacttta tagaaaatcc taagtatctt aagaagtcat taaacaagct
      121 tgctaaatta caaagagaat tatcgagaaa aacaatcggt agtttaaata gaaataaagc
      181 aagattaaaa gttgcaggac ttcaagaaca tatagctaat caaagaaatg attttctaca
      241 aaaactatct actaaattaa taaaagagaa tgatattatt tgcatagaag atttacaagt
      301 aaagaatatg attagaaatc gtaaactatc tcgcttaatt tcagatgtat catggtcaga
      361 atttattcgt caattagaat acaaggctaa ttggtatggt agaaaaattg taaaggtagg
      421 taaattcttt gcaagttcac aaatatgcaa taaatgtggg tataaaaacg aggaagttaa
      481 ggatttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg atagagatat
      541 aaatgcaagt ataaatatat taaaagaagg attaagacta ataacgattc aaaataaata
      601 acttatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt aagacatatt
      661 ttaatatgca aaattctgtg aagcaggaac cctgtgactt tagtcatggg aggttcagtc
      721 aaaggtatct agtatagttt taactcaaga catgatgaat agaaataata ttagctttaa
      781 tgatgttgat ggaataacaa catatacaag agacataaaa ggtatagaag ttggaatttt
      841 atttaaagag aaaaaacaaa atgaaattaa agtgagtttt aggtcaaaaa attatgttga
      901 tgtaagtgaa atagccaagt tatttggtgg tggtggtcat gtaagggctg ctggatgtac
      961 tattagagat agtattgata atgcaaagaa aatggtacta gaggcagtgt taaaatcaat
     1021 ttaaggtgac agtaaaatga ataagattat aagtatattg aaacccacag gtatgacatc
     1081 tcatgatgtt gtaagtagag tgagaaagat tttaaatata aaaaaagttg gtcatacagg
     1141 tactcttgac ccagatgctt caggtgtttt accaatctgt atagggaaag ctacaaaagt
     1201 atgtgaagtt attttaaaca aagataaatc gtatatatgt gagcttactt taggtataag
     1261 tacagatact tatgatgctt caggtgaaat tttaaaaaaa gttgatgatt ttaaatttag
     1321 taatgaagac atagaaagag cttttgacac acaaagagga gagataaatc aacttcctcc
     1381 aatttattca gctttaaagg taaatggaaa gagaatgtgt gatttagtaa gaagtggtag
     1441 acaatctgaa attactttaa aaacaagaag agtaaatata aaagacataa aaatactaag
     1501 tataaaaggt aataaagtaa tgttttatgt tgagtgttct aaagggactt atgtgagaag
     1561 catttgccat gatataggag aatatctagg ttgtggagct catatgtcgt ttttgaatag
     1621 aacatcttct ggtaaatttg acttagataa ttcaataaca ttagaagaat tagaattatt
     1681 ttatgaaaat aaaactttag acaaatactt atatgatata gattatgtcc ttgattcgtt
     1741 taattatgtt gtattaaacc ctaatgctat aaaatattat agtaatggtg gctcaataga
     1801 tgataaaagg tttttaaaaa ataatttcga taaagatgat gaatttgtaa gagtatacag
     1861 tacagataat ttcttaggat taggaaaatt atcaaaacac aataatacaa taagtgttaa
     1921 aagtgataag atgttcatat aaccaatgta tttaaaaaaa caacaaaaga gatagtacta
     1981 taataacttc tatagtctat ctcttttact tatgctataa tagtttgtga ataagttaag
     2041 agtaaattat attttagcgg ggaacattta ttatggtgat tataaaatct ataaaagaaa
     2101 tagcaaacat aaaagaaagt gtcattacca taggaaattt tgatggtgta cacaaaggac
     2161 atcaagttct tataggaaaa acagttgaac atgcaaaaaa agaaaatata cagagtatcg
     2221 tatttacatt tgcaaatcat cctgtaaatt actttagacc aaactcaaca aaaaatatta
     2281 taagcaatga tgataaaata aaattattag aagatttggg aatagatata gttgtaatca
     2341 taccatttga tgagtatatg actaaaatac ctgcaaagga tttcgtagat gagatactag
     2401 taaaaaaatt aaaagctaaa aagatagtaa ttggacatga ctttacattt gcaagggcaa
     2461 aagaaggcaa tgttaatcta ttaaaaagct tagaacatga atttggtttt gaggtagaag
     2521 taataaaacc catcaaaatc aatgatgtaa gagtgagtag tacttatatt agaagtttag
     2581 tatcccaggg tgatatggct aatgtcaagg aatatttggg tagaaattac aagttagaag
     2641 gttgtgttat acattctaaa caccttggaa gaactatagg attcccaacg gcaaatattg
     2701 accttaagaa taatatgctc gttccaaaga gaggtattta tgcttctatt gtacatattg
     2761 gagatgagac ttattttggt gctacaaatg taggctataa cccaacagtt aatggaaaaa
     2821 ctttgtctat tgaaacaaat attttagagt ttgatagaga tatttatggg gaaaatataa
     2881 ttgttgaatt tttggaaaga ataagagatg aaagaaagtt taattcaata gatgacttaa
     2941 aaaatcaatt gtataatgat acaaattatg tgtatgaaaa ctatgtttgc aaaaacaagt
     3001 aatgtatgat aaaatgatta aggaaaataa ccattactca gcatttgaat actccaatca
     3061 atgcttagta ataggcgttt ctaaaaaaat acggaggtaa tctaaatgat aaacaaatca
     3121 gaaataataa aagaatatgg aagagctgaa ggggatactg gttctccaga agtacaaata
     3181 gctttattaa cagctagaat aaacgagtta aatggacatt taaaaactca taagaaagac
     3241 caccattcaa gaagaggact attaaaaatg gttggtagaa gaagaaactt attagcttac
     3301 ttaaaggaaa aagatcttga aggatataga gcattaatag ctaaattagg attaagaaag
     3361 taatttcatt ctttataaaa aagcaggtat agtgtatacc tgctttttta tagttaattc
     3421 attataaagt aaaatagtta ataataaaag tagtttatat gttataaaaa attataaaat
     3481 gaataatatt gggtaagaat attaaaatag tatacaaata cataatcaat ttaaaaggaa
     3541 agacaggata aatatagaaa ttttataaca ggataattag ttggaataag gaggatatta
     3601 ttttgaaaat aaagttagta tgcgatagtt tgtgcgatat tccagatgaa atatcagaaa
     3661 aagattatat agagatagtt ccacttacag ttatttttaa tgatagggaa tatatagaag
     3721 gagtagatat aaagaaagaa gagttttata aaaaagtaaa agaaatcaag caaataccta
     3781 aaacttcaca agctacatat atggagttta aagaagtgtt tgataagttc ataacagaag
     3841 gttatcatat aatctgtatg acaggggctg caaacgcatc aggaactttt caaagtgcta
     3901 tgatagctaa aaatgatgta aatgaaaatg aaaaaataca tatatttgat actagaaatt
     3961 tatctctagg tagtggacaa tatgttataa aggcatgtga acttcttgaa gaaggactgg
     4021 gatttgaaga aattatagat gaattagaaa atactcgcag tagtgtaaga ttgctttttg
     4081 caccatatac acttgatttt ttaaagcaga gtggaagagt tccagttgca actgctttga
     4141 taggaaatat gttaaatata aagcctatat ttttctttga taatggggaa gctaagttgg
     4201 taaataaggt tagaggaata aagaatatag caagtaaatt ggtcgatata attttagaaa
     4261 tgaatgaagg gtctttagaa ggaaaaatcg tcacaatagg ttgtggagat aatttacacg
     4321 attgtgaaat attaaaagat gaagtaaata aaagaataaa agcaagaaga gtattattta
     4381 ctcgaggagg agtttctatt tgctctcata cagggccaga tatattggct ataagttgtt
     4441 ctaattagta gtaatataag gtttttgcat atatatgtga agataatttt atattgttaa
     4501 tttatagtat ttttgataat atagtaaata gagtgatata cgtgtttttt aataagagga
     4561 ggacaagcat taatgtttga acataaaata tttaaaatgg attttgcagg aagagaactt
     4621 tctgtagaaa taggaaagat ttgtgaaatg gctagtggta gttgtatagt tcgatacagt
     4681 gatagtatgg taatggtaaa tacaactaaa tcagccaaac ctagagacgg aatagacttt
     4741 tttcctttaa gtgtggatta tgaagaaaaa ttatactctg taggtaagat acctggaggt
     4801 tttttaaaga gagaaggcaa gccttcagag aaagctatac ttacatcaag attgatagat
     4861 agaccaataa gacctttatt cccaaaagga tttagaaatg atgttcaggt agttgcaaca
     4921 gttctatcag tagaccaaga ttgtacacca gatatagtag caatgatagg ttcttcaata
     4981 gccctttcta tatcagacat accatttaat ggaccaacag gttcagtatg tgttggcctt
     5041 gtagatggtg catttgttgt aaatccaaac gcagagcaaa gagaaaaaag ttcaatgcat
     5101 ctagttgttt caggtacaaa ggaagcaata atgatggttg aagccggagc tgatgaagtt
     5161 ccagacgagg taatgcttga tgctatttta tttgctcacc aagaaataaa aaaaatagtt
     5221 gagtttatag aaggtatagt tgcagaagtt ggaaaagaaa aaatgcctgt tgaattatac
     5281 catgcaggag aagaaataac tcaattggtt cgcgaatttg caactgataa gatgaaaaaa
     5341 gcagttcaga cttttgaaaa gttagagaga atggaaaata tggatagagt taaagaagaa
     5401 actttagctc actttgaaga aactttagaa gattttgaag attttgttgg agatatagaa
     5461 gaagtattac aagatataat aaaagaagaa gtaagaaaac ttatagtaca tgaaaatgta
     5521 agaccagata atagaaaact tgaagaaata agacctatat ggtgtgaaac tggcatgata
     5581 cctagagctc atggttcagc catatttact agaggacaaa ctcaagtact taatgttgct
     5641 actttaggtg ctttaggaga tgttcaaaaa ctagatgggt tagatgaaga agaaaacaag
     5701 agatatatgc atcattataa tttccctgca tatagtgttg gagaagctag accatcaaga
     5761 ggaccaggaa gaagagaaat aggacatggt gctttagcag aaagagcttt attaccagta
     5821 ataccatctc aagaagaatt tccttatgct attagattag tttctgaagt attaagttca
     5881 aatggttcta catctcaagc aagtgtatgt ggctcaacat tatctttact tgatgcaggt
     5941 gtacctataa aagatatggt tgcaggtata gcaatgggac ttataaagca tgatggtaag
     6001 gttgcagtgc tttcagatat acaaggtatg gaagaccatt taggcgatat ggactttaaa
     6061 gtagctggaa cagagtatgg tataacagca atacaaatgg atataaaaat tgatggtata
     6121 gataaagaaa ttctacaacg tgctttaaaa caagctaaag agggtagaat ccatatacta
     6181 ggagagatga gaaaaactat atctcagcca aaaccagaac tttcaccata tgcacctaag
     6241 attgttaaaa tgcaaataaa tcctgacaag ataaaagatg taataggacc tggtggaaag
     6301 ataataacta agataataga cgaaactgga gttaagatag atattgaaca aactggggaa
     6361 gtatttatat caggtattga gattgatatg attaagaaag ctcaagaact tataaataat
     6421 atagtagttg aaccagaagt aggaaaaact tataaaggta aggtaagtag aataatgaac
     6481 tttggagcat ttgttgagat acttccagga aaagaaggat tacttcatat atctcatata
     6541 gctcatgaaa gagttgccaa agttgaagat gtattgaata taggggatga agttgaagtt
     6601 aaagttacag agattgatga aaaaggaaga gttaatttat caagaaaagt tcttttacca
     6661 aaaccagaac ataaaaataa ataacttatt atataattaa aagcaaagtc ttaggatttt
     6721 gcttttttaa atacaaaaaa gtagataatt ttaaaatgat agttatggat ggcttatagt
     6781 tctttttctg tcaggcttta ctcttataaa gaagctattt aaaagctgat taatagagag
     6841 ctttattgat atatatagaa tcacttaagt tatatttagt gcataaacag agcttatttt
     6901 taatagtatt aaataagaaa caatttgtac ataattaggg ggagttttaa tgataatgat
     6961 ggtacttaat aagagtaagc tcaaaaaaat cataatacta attttagtag tgttattagt
     7021 attatcagga ggggtaatgg tattaaagtt atttaacaac aagccagtat ttaatttatt
     7081 taatggtgta gatttaccat atgaaagagg tacaaaggat aaaagtggat atgttgcatt
     7141 aacctgtaat atagaccttg gatgggaaac agagtacgta gaatccattt tagaaacact
     7201 taaaaaagaa aatgtaaaga ttacatttaa tgtaactgga aaatgggcag aaaagaataa
     7261 ggatgaatta ttaaagataa aaaaacaagg acatgaaata ggtaatcatg gatacaaaca
     7321 tttagattat tctacattat catatgaaga caattatgag caaatagaga cctctaaaaa
     7381 gataattgaa gaaattatag gtgaaaaaac taaatttttt caagcaccag caggttcttt
     7441 tggtccagaa acagtaaagg ctgctaaagc attagggtat acttctatca aatgggatgc
     7501 cgatactata gattggaaat acaaagacca accagaggta atcattgata gaatgaagaa
     7561 aaaagacatt aaagatagta gtataatttt aatgcatcca accaacgcaa cgacaaaatg
     7621 tatagatgat attatagcta ttgtaagaga aaaaggacta aaaccaggaa aacttagtga
     7681 tgtatttaag tagacaaagt ataaatattt ggttaagatt gtataagttg tagttgaatt
     7741 aattaaaaga ctgacttata atgtaataca tataaaaaat aaatataatt aaaacatagt
     7801 gttttcaata atccaatatg aaacaaagaa taaattgtta ggagaatgta atgtataaga
     7861 caaaaatact ggaaaatggg cttacaataa ttggtgaaga aataccatat ctaaagtcaa
     7921 taacattagg tatttggata aatgcaggct ctaggataga agaagcacag gtgagtggaa
     7981 tttctcattt tatagagcat atgatgttta aaggaaccaa aaacagaaca tcaaaagaga
     8041 ttgctagttc aatagataat ttgggtggtc aaataaatgc atttacaagt aaagaatgta
     8101 catgttatta tgtaaaacta atagatgagc atatagatac aggaattgat gtattgagtg
     8161 atatgatact aaattctaaa tttgataaaa atgatataga taaggaaaga cttataatat
     8221 tggaagaact taagatgtat gaagactctc cagatgactt atcttatgat ttattagtgg
     8281 aaaatatata tgctaatgat ggattaggaa tgaatataat tgggacaaaa gaatctctgt
     8341 acaatataac tagagaatcc atgttagaat acttaaataa gtattatatt ccaaataacg
     8401 ctgttatttc tatagctgga aactttaact ttgatgatat ggtggagaaa atcaaatcaa
     8461 aatttggaca ctgggaaaag aaaaatttaa gtatagacat aagtgaggct aagtttaatc
     8521 cttgctttat atcaaaaaat aaagatactg aacaggtgaa cttagctatg tgcctaaaag
     8581 gtattccttt tgaaaatgat gaagaagtct attcaatggc agtggtaaat aatattttcg
     8641 gtggaagtat aagctcaagg ctttttcaga aaataaggga agaaaaaggt ttagtatatt
     8701 caatatactc atcacagaca ttatatagga aatgtggtga attaggtata tttgcaagca
     8761 tgagtacaga aaatttacaa gatgtatata atttaataaa gaaagaaatt gaaaatataa
     8821 gagaaaacta tcttacagaa aaagaaatat ctgaaagcaa ggaacagtta aaaggaaatt
     8881 atatattgga tttagaaagt actagcagta gaatgatgtc tactggtaaa tctatgttac
     8941 ttagcaaaaa agtaaaaact acagatgaaa tacttgaatg tataaataat gttaatatta
     9001 attcaataaa gaaggttgtt gataaggtat tcaatatcga aaatattggt acatgtatag
     9061 ttggaagaga tgtagaaaag attctacact tggattaata ttgaaaaaag taaatctctg
     9121 gaggtataaa atatgaattt atctgaaata gcaggtaagg aaatagttaa cttggtcaca
     9181 ggagaaagat tgggggtcat tggagaatgt gaccttatac tagatgaaac tacaggcgat
     9241 atattagcgc ttctaattcc aaaagaaaaa ggattttttg ccagaagaaa agataagtca
     9301 tttttagaag taccatggag aaatgtaaaa aaaataggta atgacatgat aattatagaa
     9361 tatgagggat atatttagga ggaaaaaatg agtattttag tgcaaaagtt tggtgggaca
     9421 tcagtagaat catatgaaaa aatgaatgaa gtatgtaaga tagtaaaagc atacaaaaaa
     9481 aatgatgaag aattacaatt agtattagta gtttcagcaa tgggaagaaa aggagctcca
     9541 tatgctacag atacacttat aaatttgtgt agtgcagtaa atgatgagcc aagtaaaaga
     9601 gaacttgatt taataatgtc ttgtggagaa ataatatcag gaacaattct tgctaattta
     9661 ttgaatgcac aggggataga ttctgttttc tttactggtc agcaagcagg tataattaca
     9721 agtgatgaat attctaatgc aaagataaag tatataaatc ctaaaaaggt aaaaagagct
     9781 ttagatgatg gtaaggttgt agttatagct gggttccagg gtgtaacaga tgatggagaa
     9841 ataactactc ttggaagagg cggaagcgat acatcagcag ttgctattgg taaagcttta
     9901 gaatgtgaaa cagttgaaat ttacactgat gtggatggta taatgactgc agaccctaga
     9961 gtagaaccaa acgctaaagt attgagtttt atagactatg aggaagtatt ccaaatggct
    10021 gacaaaggtg caaaagtaat acatccaaga gcagtacagt tggctaaaag tggaaatata
    10081 actttagcca taaagaatac aatgaatcca acttttgaag gtacaaaaat aggttcttta
    10141 tgtagacact tagaagacaa tatagaatat gaacaagaaa gagattttaa ggttgctgtt
    10201 gcaaataaag atagcgtagc acaagttaag attaagtcag cagaagaagt gtttacagaa
    10261 gtattaaatg agatagaaaa gaaacttata actatagata tgataaattt ctttatatca
    10321 gaaaaagcat ttgttgtaga agatgctgat ataaaatcat taaaggaaat actagataaa
    10381 tttgaattag attatgaagt aaatagagac tgtgctaagg taacattgat atgttcaaga
    10441 attgctgatg aaatgtctgg tatcatgtca aaggtagtta gaggattatc aaaagcaggg
    10501 gtatctcttt tacaaacatc agactcaaat atgactatat cttgtttagt tagtgaagaa
    10561 gatatgcata cagcagtaca tgctatacac caacaatttt atttaaaata aactgtataa
    10621 tacaattatt ttttaatcaa atttaagaat ttcatatata tcttaatata tttgatttat
    10681 ttggtaaaac taaattaaat ataattttga taggagtttt tttatgaaag attatagaga
    10741 agataatgaa aaatttgata aagataattg tagtgaagaa tctagttgcg aagagagaga
    10801 atgtataaag caatttggta ccaatgaaat gccacctcaa cctcctaaag atattcaatg
    10861 tataactata attggagaaa ttgagggaca ttttatagga aatcctcaaa aaaaagctac
    10921 aaagtacgag catataattc caatgctata ctcaattgag gaaagtaatg atgttaaagg
    10981 agtgttagtt gttttaaaca ctgttggagg agatattgaa gcaggtttag ctattgcaga
    11041 acttttaaac agtacatcta aaaaagtagt tactcttgtt ctaggaggaa gtcatagtat
    11101 aggtgttcct cttgctacag caggagatta ctcatttata gcaccaacag caaccatgat
    11161 tatacatcca gtgcgaacta ctggtcttgt gattggtata aatgagactt ttgaatattt
    11221 taaaaagatg caagatagaa taattcaatt tataataaga acatcaaata tcaaaaaaga
    11281 tgtacttgaa aaacttatgc atgaaaaaga tgaacttgta agtgatgttg gtagtgtatt
    11341 aataggaaaa gaagctgtgg attatggtct tatagatgaa gttggaggac taaaagaagc
    11401 acttaaaaaa ttaagagagc ttataaaaga atctgaggaa gaaaaaaata atgattaaaa
    11461 tttgtttaaa taataaaatt tatgatataa tgatgtaata aagtaggagt gttagtgtta
    11521 aggcattagc actttttgct gtgaaggggt aaaacttgct aaaaataagg agtgatatta
    11581 atggccacga aaaagaagaa aaaaaagaag gtaaatacct taagttttaa tgcagaatat
    11641 cataatttaa taacaatatt tataggtgta tttttattat atagtttaaa ttctaattct
    11701 atgggatgga tacctgtgtt aatgcaaaat ctttttaagg gactatttgg agggctttca
    11761 atagcaatac catttatagt aattataaca ggacttttag gattttttga tggaaatgaa
    11821 tatatatata ggctaagaaa gactaaacta tactacataa ttattttatt tatatttgta
    11881 ttttatggat tattaaatgc aggaactctt cctgtagata gcccactaaa aggtaacatg
    11941 tttgatgatg ttatgaaatt gggtgtaagt ggacaaggta gtggtttgat agcaacaact
    12001 attgcgtatt atatgagtaa aatatttggt atagcaggtg gatggttaat aagtatcttc
    12061 gcacttatac tttctgtaat gtttatattt aatatttcta taaaagattt ggtgtcaaat
    12121 gcaaaatcaa aatctaaagc atctaaagac agcaatttaa cgtttaaaga taaaattgca
    12181 aatatgaaaa aatctgcaat agatatgatg actgatgaag tagatgatac tactataaat
    12241 aaaaaaccag gtttctttaa agggttaatg tcaaaaggtc ttaataatga tgatgaagaa
    12301 gatgaatatt ttgaagaaga aaatagtgat ggtgtagatg ataaaactat aaaaatagtt
    12361 ggatttaata aggcagaaga tgagtattta gaaatattag aaggaactca aagtatgcca
    12421 gagttagatg ttttaaaaga attacaaaaa gctaccaatg aaaatccagt tgtagatact
    12481 aaaccagaaa aaaaagttga tatagctaaa cctaatttaa atatagaaaa aactcaacct
    12541 atgagtattg tagcagagcc agttaatgag gattattcaa actataaaaa gcctagcatt
    12601 gagcttttaa ataaagtaaa taagaagtct gatgaaaatg gtaaaaagaa agtgttgaaa
    12661 aatgcttctc ttttagaaaa gacactatca gattttggag tggaagcaaa aattaatcaa
    12721 gttacagttg gacctactat aacaagatat gaaatacaac caagtccagg agtcaaggta
    12781 agtaaaattg taaatttaac tgatgatata gctttaagtt tagcagctaa aagtataaga
    12841 attgaagccc caattcctgg taaaagtgca attggaatag aagttccaaa tgaagaagct
    12901 caaatggttg gtgtgagaga agttctagaa agtgaagaat ttaataattt taactctcct
    12961 cttgcaatgg gacttggaaa agacgtagca ggaaaaatta taattggaga tataggtaag
    13021 atgccacact tactaatagc tggttctaca ggttcaggaa agagtgtgtg tgtaaataca
    13081 ttaataagta gtattttata taaggcaaat cctgatgaag taaaattact attaatagac
    13141 ccaaaagtag ttgaacttgc aaattataat ggaataccac atctattgat accagtagtt
    13201 actgacccta aaaaagctgc aaatgcttta aattgggcag ttacagagat gaatagaagg
    13261 tacaaattat ttgcagatgc tcaagtaaaa gatgtaacaa gttataatga gaaagcggaa
    13321 gaaaaacttc ctaaaatagt aataataata gatgagttag cagatttaat gatggcaagt
    13381 gcaaatgatg tagaagatta tatttgtaga ttagctcaaa tggcaagagc tgctggaatg
    13441 cacttgattg tagcaactca aagaccatct gttgacgtta taacaggggt aataaaggct
    13501 aatataccat ctagaatagc atttgcagta tcatctcaaa ctgattcaag aacaatcctt
    13561 gatatgggtg gtgcagagaa gcttctaggt aaaggtgata tgttattcta tccacttggt
    13621 gcagcaaaac cagttagact tcaaggggca ttcatatcag agagtgagtc ggaaaaagta
    13681 atcgattttg taaaaagtca ggttaaagat ggaataaaat atgaagaaga tataattgaa
    13741 actatatcta aggttaatac ttcaaagggt agtgatgagg atgagttttt gagtgaagcg
    13801 atagaatttg tcgtggaaag tggtcaagca tctgcctcta tgctacagag aagatttaaa
    13861 ataggattca atagagcagc tagacttata gattctatgg aagaaagagg aataattgga
    13921 gcaagtgagg gaagtaaacc gagaaaggta ttaataagca aacaggattt gcagaattta
    13981 gaaggtgaat agaattgtca gttataaaaa tagaaaaagc acttaagcta gataaagtaa
    14041 tatttgtaga tgtgagaact gaaggagaat atgaagaaga ccatatattg aatgcaatca
    14101 atatgcctct ttttaaaaat aatgaacata atgaagttgg aacaatttat aagatgcagg
    14161 gaaaacatga agcaatacaa aaaggatttg actatgtatc gtataaatta aaggatatat
    14221 atttacaagc agcagaactt gcattaaatt atgataatat tgtcatatat tgtgcaagag
    14281 gcggaatgag aagtggcagt atagtaaatt tattatcatc attaggtgta aatgtatatc
    14341 aacttgaagg tggatataag gcttatagaa attttgtttt agagtattta aggcatgtaa
    14401 tggatacaaa aaactttgta tctgttcatg gattgactgg tacaggaaag actgatttgt
    14461 tacatttatt agatgaaaaa ggaatagacg tgctggattt agaaggcata gctaaaaaca
    14521 gtggttctac ctttggtttt ataacttttg ataaaaagcc accatctcaa aagaactttg
    14581 aaacaaaact atttgaaagt atattttttt cgaaagagaa ttatatattt gtcgagagtg
    14641 aaagcaaacg agttggaagt gttacagttc caaatgaaat tttcgaggca atgacacgag
    14701 aaggacatca tatattatta gaatgtgata ttgaaaatag agttgataga ctttgtagag
    14761 attatattta tgataaagat gaaggtaata tattgatttt aaaagaatgt ataaacaagt
    14821 ttagaaaaag attgggtaat aaagtagtag atgattatat tgatttatta gaatcaggtg
    14881 aatataaaga acttgtaaaa agatatcttt tagaatacta cgacccactt tatatgcatt
    14941 cagtagagaa atataagtat agtaagatta taaattttaa tgatacacaa aaagctttag
    15001 aagatgttat agacttatat gagagtatat tggaaggaga aagtgaatgt taaaaatagc
    15061 attggaatca ttaggatgct caaaaaattt agtagatgca gaaataatga tgggaatttt
    15121 aaataacaaa ggctataaat taattggaga ttttgaagaa gctgatgtaa ttatagtaaa
    15181 tacatgtggg tttatagagt cagctaaaca agaatctata gatacgataa taaactttgc
    15241 agagttaaaa aaaactggca atttgaagtt gttgatagta acagggtgtc tagcccaaag
    15301 atattctgaa gaattaaaaa ctgaaattcc tgaaatagat gctatagtag gaacaggaag
    15361 ctatcaaaat atagataaaa tacttaaaga attaagtgaa atgcatcaaa ttgtaagcct
    15421 taatgatata gagtttgtat ttaatgaaga tttaccaaga tatatatcta cacctagcta
    15481 tatggcctat ttaaaaattg gagaaggatg ttccaataat tgtacttatt gtataatacc
    15541 aaaactaaga ggtaaatata gaagtagaaa atttgaagat ataattaaag aagctaagaa
    15601 attagctgaa tctggagtaa aagaacttgt ggttatagca caggatacga caaagtatgg
    15661 ttttgattta tatggaaaag aaagattatc agaactttta gaagaacttg caaaaataga
    15721 tggatttaag tggataaggg ttatgtattc atatccagaa tctataacag aagaattgat
    15781 acaagttata aagaaatatg ataatatatg tagttacttt gatatgccaa tacagcatgc
    15841 aagtaacaat attttaaaat taatgaatag aaaaactact aaagaagata tattaaataa
    15901 aatcaatctg ataagaagta atataccaga tgctatactt agaactacaa taatagtagg
    15961 gtttcctggt gaaacagaag atgacttcaa acaacttgtg gactttgttg aagaggttaa
    16021 atttgataga cttggtgcat ttgcatattc aagagaagaa gatacaccag cagatagatt
    16081 gccaaaccat atagatgaag aagtaaaaat acaaagaaga gatactttaa tgatgattca
    16141 acaaaaaata tctgaagaac ttaatgacaa aaagatagga aaaacttatg aggtgttaat
    16201 agaagaacag atagaagata atgtatatac aggaagaaca caaggtgatg cagaagaaat
    16261 tgacagtata gtgtatgtaa aaagtgtaga caatcttgaa gtaggagaat ttgtaagtgt
    16321 tcaaataaat gatgcaatgg aatacgattt aatgggagat gttttatatg aacttgccta
    16381 acaagttaac tttgtttaga atatttttaa taccagtatt tgttttaatt atgttgctca
    16441 atgtaccaaa taaattttta atagcttgta ttatttttat aatagcttca attactgatg
    16501 ctttagatgg aaaaatagct agaaaataca atctagtcac tgactttgga aaatttatgg
    16561 acccattagc agataagtta ttggttattt cagcacttac atgtatgata gaagaccatc
    16621 ttgtaagtag ttggatggta attataatag tggctagaga gcttacagtt agcatattaa
    16681 gagctatagc tgctgctgat ggaaaagtaa ttgctgctgg aaacagtgga aaacttaaaa
    16741 cgataactca aatggtatct atagtgtttt tattgttagg tgctcagttt gaaaatgtat
    16801 tgatattaaa tattggtgaa attctcattc ttatagctac actacttaca ttatattctg
    16861 gatgggaata tctatataaa aataaagagc tttttatgtc ttctaagtaa aaaattatgc
    16921 tttaataaat aaaaactaat gattaataaa taaaaaataa caacttaaaa ttttaaaaat
    16981 gtaaaaaagg gtatactatt atagtatacc tcttttcatt ttaagaatat tttcttttgt
    17041 gtatataatt attaataatg gtttataaat aaaatgtaat gaataaaaat ataatcatta
    17101 gtagcgtttt taaattgact aggtttgatt tatgttatat aattaatata taacaagaga
    17161 acaaatgttt gtagataaat gaagggatgg tatagagatg agtgtagatc aagaaaaatt
    17221 aaaagcgttg aatgaagctt taggtaaaat tgaaaaagat tttggtaaag gttcagtaat
    17281 gaaattggga gaagcaacat ctatgtctat agatgttata tcaacaggag cgattggttt
    17341 agacatagct attggtatag gaggtctacc tagagggaga atagttgaag tatatggtcc
    17401 agaatcttct ggtaaaacta ctgttgcgct tagttgtgta gcatcagctc aaaaagatgg
    17461 aggaatagct gcatttatag atgcagaaca tgcacttgac ccagtatatg caaaagcttt
    17521 gggtgtggat gttgataacc taataatatc tcaaccagat acaggtgaac aggctttaga
    17581 gatagcagag gcattgataa gaagtggagc gatagatata atagtaatag actcagtagc
    17641 agcattagtt ccaaaggctg aaatagatgg agatatgggt gattctcatg taggtttaca
    17701 agctagactt atgtcacaag cacttagaaa gttaactggt tcaattaaaa aatcaaattg
    17761 tgttgctata tttataaacc agttaagaga gaaagtagga ataatgtttg gaaacccgga
    17821 aactactact ggaggacgtg cactaaaatt ctattcatca gttagattgg atgttagaaa
    17881 aatagataca ataaaacaag gtgataaagt tataggtagt agaactagag ttaaagttgt
    17941 taaaaacaaa gtagcaccac catttaagca agctgaattt gatataatgt atggagaagg
    18001 aatttcaaaa attggagacc ttttagatat agctgctgat gtagatatag ttaaaaaatc
    18061 aggttcatgg tatagttaca atgatactaa acttggacaa ggaagagaaa atgttaaaaa
    18121 attcttggaa gataatttag atttaactac tgaaatagat gagaaagtta gagcatttta
    18181 caatttaaat gaagaacatg aagaatcagg tatttcagta tcaaaagaaa ttgtagaaga
    18241 ataagtaaaa atacaaaaaa ataactagta attaaagaca tgccacgctc cctattagct
    18301 tgacacaatg ttgaaaaaat tataaaatta agtgaggata aaatatctaa atttaatttt
    18361 aattattagg aatatttttt aatcatagtg atgacatttt gggattatta acgaaggagg
    18421 tggatgtcat agatagtgta gtaatgattg taataggtgc agctgttggt attatagctg
    18481 gatattttgt tagaaaaaat atatctgaag caaaaattgg acaagctgaa aacttggcca
    18541 aagaaataat agataaagca caccacgact cagaaacagt acaaaaagaa aaattactag
    18601 aagctaaaga agagatccat aagtggagaa cagaagctga aagagaaaac agagaaagaa
    18661 gaacagaagt acaaaagtat gaaaaaagag tagtacaaaa agaagaagtc ttagatagaa
    18721 aattacaaaa tttagaatct aaagaagtaa acctaagtga aaaattaaag gtagtggaaa
    18781 agaaagaaga agaagttgaa gtaataaaaa ctcaacagtt agaaaaatta gaaagtatat
    18841 caggaattac ttctgataag gccaaagaaa tcatcttaac caatgcggag agagatgtaa
    18901 gacgtgaaat gtctataatg ataaaagaga tagaaagtca agctaaagaa gaagctgata
    18961 agaaatcaag agaaattata ggatatgcaa ttcaaaaatg tgcagcagat cacgtagccg
    19021 aaactactgt aacagtagta agcttgccta atgatgaaat gaaaggtaga ataataggta
    19081 gggaaggtag aaatataagg actttagaaa ccttaacagg tatagacctt ataatagatg
    19141 acactccaga agccgtaatt ttatcaggat ttgatccaat aagaagagaa attgcaagaa
    19201 ttgctctaga aaaattaata gcagatggaa gaatacatcc agctagaatt gaagaaatgg
    19261 ttgaaaaggc gagaaaagaa gttgacaata ttataaaaga atatggtgaa caagctgcat
    19321 ttgaaactag tgtacatggg attcatccgg aattaataag attattaggt agacttaact
    19381 atagaactag ttatggacaa aatgtactta aacattctat agaagttgct catatagctg
    19441 gtataatggc tgctgaaata ggagcagata ttagattggc taagagggca ggtttattac
    19501 atgatatagg taaggctgta gaccatgaaa tggaaggtac acatgttgaa ataggtatgg
    19561 atttacttag acgttataaa gagtctaaag aagttataca tgccatgagt actcatcatg
    19621 gagattacga gcctcaaaca gtagaggctg tattagttac agcagcagat gcaatatcag
    19681 ctgctagacc tggtgcgaga agagaaactt tagaagcata tataaagaga cttgaaaaat
    19741 tagaagaaat agctaattca tatgaaggtg tagaaaaatc atttgctata caagctggta
    19801 gagaaataag aataatggta aaaccagaag ctataaatga tgaagaaata catctattgg
    19861 ctagagatat gactaagaaa atagaggatg aacttgaata tcctggacaa ataaaagtaa
    19921 gcataataag agaaacaaga gctatagagt atgcgaagta aaagaaataa tatctaaata
    19981 ataagaaaat atttttagaa atttaaattt gccacaagat atgataatat atcttgtggc
    20041 ttttaaattg tttaagttta attttatata atgcaaaaat aatctaagtg ctaaaagtgc
    20101 tacttacaaa agattaataa aataaagctt tatatatgta ttatttatat aattatccaa
    20161 gtttgtttga tataatgaag ttgtaaaatc aatactttac tacaaaataa atagtaaata
    20221 ctacagtaaa atctaaaaat aggatataat
//
LOCUS       DAMKMI010000067        19589 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMKMI010000067 DAMKMI010000000
VERSION     DAMKMI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.068"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(305..1177)
                     /locus_tag="PC429_002170"
     CDS             complement(305..1177)
                     /locus_tag="PC429_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3847673.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(1571..2149)
                     /locus_tag="PC429_002171"
     CDS             complement(1571..2149)
                     /locus_tag="PC429_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3847674.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            complement(2207..2923)
                     /locus_tag="PC429_002172"
     CDS             complement(2207..2923)
                     /locus_tag="PC429_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF3847675.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            3114..4199
                     /locus_tag="PC429_002173"
     CDS             3114..4199
                     /locus_tag="PC429_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF3847676.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            complement(4354..5013)
                     /locus_tag="PC429_002174"
     CDS             complement(4354..5013)
                     /locus_tag="PC429_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847677.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNSNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(5230..6522)
                     /gene="lysA"
                     /locus_tag="PC429_002175"
     CDS             complement(5230..6522)
                     /gene="lysA"
                     /locus_tag="PC429_002175"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF3847678.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            complement(6540..7733)
                     /locus_tag="PC429_002176"
     CDS             complement(6540..7733)
                     /locus_tag="PC429_002176"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF3847679.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            complement(8094..8711)
                     /locus_tag="PC429_002177"
     CDS             complement(8094..8711)
                     /locus_tag="PC429_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847680.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            complement(8881..10581)
                     /locus_tag="PC429_002178"
     CDS             complement(8881..10581)
                     /locus_tag="PC429_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847681.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            complement(10978..11541)
                     /locus_tag="PC429_002179"
     CDS             complement(10978..11541)
                     /locus_tag="PC429_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847682.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            complement(11700..12413)
                     /locus_tag="PC429_002180"
     CDS             complement(11700..12413)
                     /locus_tag="PC429_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF3847683.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            12705..14369
                     /locus_tag="PC429_002181"
     CDS             12705..14369
                     /locus_tag="PC429_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF3847684.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(14476..15375)
                     /locus_tag="PC429_002182"
     CDS             complement(14476..15375)
                     /locus_tag="PC429_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF3847685.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(15402..16040)
                     /locus_tag="PC429_002183"
     CDS             complement(15402..16040)
                     /locus_tag="PC429_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF3847686.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(16381..17379)
                     /locus_tag="PC429_002184"
     CDS             complement(16381..17379)
                     /locus_tag="PC429_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF3847687.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(17477..18052)
                     /locus_tag="PC429_002185"
     CDS             complement(17477..18052)
                     /locus_tag="PC429_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847688.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            18368..18631
                     /locus_tag="PC429_002186"
     CDS             18368..18631
                     /locus_tag="PC429_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847689.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNDENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(19068..19445)
                     /locus_tag="PC429_002187"
     CDS             complement(19068..19445)
                     /locus_tag="PC429_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3847690.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         6439 a         3211 c         2044 g         7895 t
ORIGIN      
        1 atattttaat agaattttaa taagatatta tatacttaaa tacaaataaa attaatttta
       61 taattgttaa ttaaggaaat gatattgttt agagaaataa aaaaagcact ctccatgaag
      121 ggaaatgctt tctttaataa attagttgat gtagtaaaga tatattcaga atacatcttt
      181 atgttttgag tctcgaaata tttatttgta ttatttaaac gaaaatttat tttttaagtg
      241 ataatctagt atttattaat aaggctatga ttctcaattt catagcctta ttaatacaca
      301 aactttactc atacacagaa tatcttttcc aaatttgctc taaatcatca aaatgctgtt
      361 taatatcttc tttttccttc atcccaacag atatagcaag agcatttgaa atactaagag
      421 ctggaactag agaatctaca aatgaagcca tattactctt aactagaaga gtgttatctg
      481 caagtgatgc tactggcgca aataagctat ctgttaatga tattacatgg gctccttttt
      541 ctttagcata attgacaatt tgataagatt tcttagaata acgagggaaa ctaatagcaa
      601 ctattacatc ttcttcattt attcttacaa tctgctcaaa agcatctccc atatccattc
      661 taattatatg aacattgtca agtattatat ctaagtaaaa acctaaatat tgagctacta
      721 caaaagaact cctcattcca agtatatata tttttctcgc tcttaaaagt ttgttagaag
      781 cttctttaaa cgctctctcg tcaatttctt ctagtgtaga ccttatatta tctatatcac
      841 tttttaaaac tttgtttagt attgcaaaat catcggaata atcatgtgcc atttcaactc
      901 tttgtactgt agttagttta ttttttatga gttcttgaag tgcagcttgt aatttaggat
      961 atccactata acctaatgca tttgcaaatc ttacaactgt agactcactt actcctacag
     1021 tttcacccaa tttacatgca gtcataaatg caactttatc ataattattt aaaatgtatt
     1081 gtgctataag tttttgaccc ttactcaatc tcgtatattg agattgtata tttgatatca
     1141 aatgtttact gtccttcatt tcatttgttt cttccatttt tttctccttt tagttatata
     1201 aactattttg ttttattaac actgtctttt tctctaaaaa ttttattttt tatctcaatt
     1261 tcatcttagt ttttatcata gcttcttaaa ctcataaaat tctaactttt ctgtacctta
     1321 tacttcacat agtattttcg attaaattta ttataccatc tttccttgca ttttttaata
     1381 tttatccaat atttctttta aatttttcaa tttttactca tttaaaagtt atctatctta
     1441 ttttgcatat ttagatgtat attttgtttt gagaataaac tgttgtatac ttattattta
     1501 aagtattttt aataggtcac actatatata ttaactctta atacaatctt gtcaagtttt
     1561 ttagtattta ttatgctttt ttcttttcaa ttaaaatcaa ttgtggtggg ttattgactt
     1621 gatttaaaaa cttagattct aaaacattaa attcatcttg atttaaatta ttgacaaggt
     1681 tatatatgca atctttttct tcttgacctc catcatgccc tgtatatatg gcaatgctta
     1741 ttactccatt ttcttttaac atttctaaac ttaactttat agcctttaaa gttgtatctg
     1801 gtttggttat aattgaatgt ttcgctcttg gaagataacc caaattaaat aaaacacatg
     1861 aaacttcttc ttttatatat tgattcattt tttcatgccc atcaaatatc aattttactc
     1921 tatctgtata tccttctttc tctagtttct ttcttgtaga ctttattgct tcttcttgta
     1981 tatcaaaagc atatacaaag cccttttctc caaccttctc agctagatac cttgtatcat
     2041 acccatttcc catagtagca tcaattacta tgtcaccttc atttataata ttttctaaat
     2101 aaaatttatt tatatctgta attttagtta aatactttgc atatgccata atactatttc
     2161 tccctactat taactttatt tcattttttc tcaatttatt tttatcttat tttattgatt
     2221 ttaaatattt tatttcatct tctgttaggt atctatattc tccaactttg gtgtctttta
     2281 atactatttt ccccacagaa atccttctta aatttaaaac tggatgattt atagctttgc
     2341 acatttttct tacttgtcta tttctaccct catgtatagt tatttcacac actgaataat
     2401 ttttttcttt attttcttta gttaccctta ttttggcagg tgccgttgtg taatcttcta
     2461 tttttagtcc tgtttcgaat tgtcttatct catcatcact tataattccc ttcacacttg
     2521 ccacatacat tttatcaatt tcatgctttg gatgggtaag cttatatgtt aaatcgccat
     2581 cattagtaag tattaataaa ccactagttt cataatctaa tctccctatt ggatatactc
     2641 tttcccttac atctttaagt atgtctatta cactgggtct attgaattgg tcttttactg
     2701 tagttatata tccctctggt ttatttaata ctatatatat ataattttca cttggtttta
     2761 cttttttatt atcaaattct actatgtctt tttcttcatc aatattaaaa gataactctt
     2821 ttactacact tccatttacc tttattcttc ctccaagaat tatttcttct gcttttcttc
     2881 ttgatgctat tccacaagaa gctatatact tattaattct catacttaat ccttccttat
     2941 cttaaattat gtgtgtttaa tatttttatc aatgttaatt ttgctacatt attatactat
     3001 actagacctt aattaaaaac aagcaaaaaa attatttaat tttcctaaat aattgtaatt
     3061 tcttttcaca tactattaat atgtaatagc tgactaaaca ctattttaag gaagtgatta
     3121 ttatttcaaa ggaaaatata aaatattatt taatagtcgt gtttattgga attttatttt
     3181 ttaaatttat taatactcca tcagacttta tatctagtat tgaaggattc ttaaagtttt
     3241 ttagcccatt tttattagct attttacttg ctttactttt aaatccactt gtgatgcttt
     3301 ttgagataaa attcaaagca catagacttt tagccatttt tttatcttat atatttatag
     3361 gttttatttt agcctttgcc attaggttat taattccatc aatagctaat acattaaata
     3421 gattgataaa tgaaatgcct atgtatactg attatataga tagttttata gaaaaaaata
     3481 tgtcaaacat atattttcta aaaactttaa ttcctcatat acaacatagt ttagataatt
     3541 tattaaaaga agcaagtaat tttgttggta aaattcctaa aaacttctta atctatacac
     3601 ttagtatatc ttctatgcta tttaatatga caatgggatt tatactatct atatacatta
     3661 tatatgacaa agaaaaaatt gcattgggat ttaagagatt tctatattct tctactgcta
     3721 gaaacaaggc agataacatt atagaatttt ttaggactac tcatgatatt ttctatgatt
     3781 atttacttgg tagaatatta gactctctta ttattggtat aattgcattt ttaggatttc
     3841 aatttgttat cagaatagaa aatgctttat ttttagcttc aataatattc ttaggtaata
     3901 taattcccta ttttggtcct tttattggtg caatccctcc aatagccatg actatattat
     3961 atagtccaca aaaaactata tgggtcattg catttctatt tatattgcaa caattagatg
     4021 gaaattttat agaaccaaaa gttatgggta atcaagttgg tataggagct atatgggtaa
     4081 tatctgctat tttggtggga caatctctat ttggtttcat aggagttttt ctttcagtac
     4141 caatagctgc tgttgtaaaa acttatgttg ataaatatat agataatcgt ttacaatagc
     4201 ttatctctac tgtgacttta atttttagtt taaaatttaa ctgttattta ttaaattatt
     4261 tctcaaaaaa tatctcttag taagaaaaaa ttgctaagag atatttttta taatatataa
     4321 tttgttcatt aaatttatgc atatatttta tacttattga ttatttgagt tttgagaatt
     4381 tccaccacta tctgaaccac cagtagtatc ttgtggagtt ggtgctggtg ttggattact
     4441 attatttggc ttttcttgat tttgagaaga actgttacta ttgtcagact tattactatc
     4501 tgatttttta tcattatttt tagaactaga tttattattt ttactacttc cactataaga
     4561 agatttcctt gcatctgatt caactctata cctatcttct ccagccgtat cttttgcctt
     4621 tttctttctt tgttcctctg cttcgttttg ttctttttct aatttagtta actcttcttt
     4681 ctttttagtt gcctcttctt taataacaga ggaaccattt ttgattttct ctataagttt
     4741 aagttccttg attgcaccct cataattttt taattcttca aactccgtca tatttgacat
     4801 tctcatagct tgcatataca tagctcttgc attctcatta gtactatctt cttccagtgc
     4861 agaggataat gattccatag cttttttata gttatgtttt tctaaatatg tttttccttg
     4921 ctctactaaa tttatttcat cttttttatt aaatgaacat cctacaagaa atatagataa
     4981 tatcgatatt aataaaatta ctcttcctct cattaaatcc ctccttgtca tatactttaa
     5041 attttatcat aattataaag tttttaaaat attttatatt tattacaaga aatttgttag
     5101 taaaacaaaa aataagcaga tatgctacga acgatacaat aaaccaaaaa ttaatttaga
     5161 gatacatatc ctagcaaatc tgctcataat aaactttaat atttaatttt tattatatac
     5221 ttctccattt tatatctcta aagctaataa gtcctcatag ctttgtctct tacaaattat
     5281 cttatcattt ccatcaaata ctgatactac tgctgctctt ggtattttat tataatttga
     5341 agacattgaa tatccatatg caccagttga tgttgttata agtatatctc cacttttaat
     5401 atccattatg tttatatctc caattaatat atctccactc tcacaacatt ttccagctat
     5461 tgttacatga ttcatagcct catgattaat tttattcaca atactacact cataactagc
     5521 ttggtacaac gaaggtctta cattatctgt catacctcca tctacactta cataaaccct
     5581 aacatctttt atatctttta tagaacctac tgtatacaat gtacttcctg catttgcaac
     5641 tattgacctt cctggttcta ttaccaatgt aggtacttct attccaagct ctttacatga
     5701 agtttctgct ttagtgatta tggtttcaca aaactctttt atagttttag gtttatctcc
     5761 ttcgctataa taaacgccaa ctcctccacc taggtcaatt tctgttagtt gtatgttgaa
     5821 tttttctttt atttctttta ccaagttcat cattatatcc actgtatcta tgtatggttc
     5881 tacatcaaat atttgtgaac ctatatgagc gtgaagaccc acaagtttaa tatttttata
     5941 ttcacttaat ttttctattg ctctaaataa atctccattt actaaagcaa atccaaattt
     6001 agaatctatc tgtccagttt ttatataatc atgtgtatga gcttctatac caggagttat
     6061 tctaaagtat atctcttgag ttttaccttt atcttcacat aatgcttcaa gcaaatctaa
     6121 ttcataaaaa ttatccacca caaatttacc tacacctaaa tctaatccca tgtttatttc
     6181 ttctttagtc ttattgtttc catgaaagaa aactttttcc ataggaaaat ttgatttata
     6241 agctgtatat aactcgcctc ctgaaactac atctaaacac atttgttctt catttataag
     6301 attacacatg taaagaggta aaaaagcttt ccctgcataa gcaaccttat tttttccctc
     6361 tctagcctta aaatattcta catattctct acaattttgt ctaactaaag cttcatcaaa
     6421 tacataaagt ggagtttgat attttttact aagttctaaa caactcactc ctccaatata
     6481 taattcatca ttatttacag tcatagttcc atgaagtttc ataatagcct ccccaaattt
     6541 tatatattaa atacttctgc tattttttct atagctactt gcttatcatc agaatttata
     6601 gcacaagtta tacgaatctc tgatgtagtt ataagtttta tcattatacc attttcattg
     6661 aatatcttaa atactttagc agcaactcca gaagtatttt tcataccaag acctactaaa
     6721 gaaaatttag tgatattatt atcaatcact acatgttctt catctgtata tttagatact
     6781 atctttttac attctcccaa ctcttctttt ggaactgtaa atgatacact tatcttatct
     6841 aaaataggtg cagtttggct tatcatatct acacttatac ctatagttgc tatatcttca
     6901 aataagctag atatattttc agctttaaaa tcttttattg ttatagaact gtcatcatca
     6961 ctagtagcta atcctgttat tactttatcc tctaatttca tgtcttttcc ccctcttata
     7021 tatgttcctt tttctgtacc acatgtacgt ccgacataga tttcaacacc atatttttgt
     7081 gcaagctcta tacttcttga atgcattact tgtgctccta aactcgctaa ctcaagcatt
     7141 tcttcatatt caatctcatc aagtttacta gcctttgaat attttcttgg gtctgtaaaa
     7201 taaattccat caacatctgt atatatctca cattttccat ttaatttaac cgcaagagct
     7261 acagcagatg tatctgaacc acctcttcca agagtggtta catcaccatc ttcgttgatt
     7321 ccctgaaatc cagttactat cacgacattt ccttcatcta aactattttt aattctgttt
     7381 acgtttatat catcaatttg agactttcca tgaagaccac tcgtttttat atttaactgg
     7441 taagcattat aacttatagc tttacatcca agtgcattta atgctattga taataaagat
     7501 gctgaaatca tttcaccagt agacattaaa gcatctaatt ccctcttact aggttcatta
     7561 ctcaattcct ttgctaaagt tatatattca tcagtggatt ttcccatggc agacacaact
     7621 attaccattt gtggattttc ttttcttcgt tctattatat tttctgctat tgattttatt
     7681 ttttcagtat ctgctactga acttccacca tatttttgaa caattatact catgctttct
     7741 ccccctcaag ctaaattttg ataaataaaa acgcccaaag gtatactttg gacgtgtaaa
     7801 tttcatagac caagtaacct tgtagctcac cactttaaaa gtgacagcct tatgcatatt
     7861 ctgcataagt ccagaaaatt tgtccgtcaa caaaatttct tcggctatat accctttcat
     7921 caattatcaa cgcttaccag ctcctattga atactattga atacagctac ctctacccta
     7981 ggctcattat ttttaattaa tttattaata tagttttatc atattctata taatattgca
     8041 acaattattt tacaaaatgt ggcaaatcga tagttttctt ttctaaagtt agcttatata
     8101 caattaaata gtttatttaa tcctaaatct ataaaattta tgctatactc attatcatat
     8161 ctctctaaat catttaattc ggatagatta tctggtgtaa ctaccaaaac gtttggtact
     8221 ttaaaaaatg gaattgtaga tttatctatt tttttagaaa gattttcgta tttagaaatg
     8281 tctatttcat tatttaagtc aacatcaact aaaacttgct ttatattacc aaattggtct
     8341 gtatattcaa taaataaatc gtatttcaca tcatctacac taacatttcg gtaaaatctt
     8401 tttacatcac aacctgcagt attgattcta acaactaatt ctgatcctac tagagccttt
     8461 attaaatcat ctcccttaaa aagtttacta ccgtttaaat agtatacata agagaggtct
     8521 ttataattat aataatccac atcacaagga tattttttta gtgtatattc actacacatt
     8581 tttctcaaag tttttttaaa atttttttta ttgtatcttt tttggtctcc ataaattttt
     8641 ctaaattgag actctgtaac acatctaacc ctgctaatta aaagtaatat ctctttttcc
     8701 atattcgtca tagtacattc tccttgaagt attatttgca aagttgtact aatattatat
     8761 taattttatg acattaaatt tacaaagaat taaaataaaa tagctaattt ttatagaatt
     8821 aaattctata aaaattagct atcactatgt taaatactat tatcaatctt tttatttaaa
     8881 ttatacatta ccttgatttt cagaatcttt ggaatctgta ttttcatcag ttcctgtttt
     8941 ttcttttgct tttttatctt caaagtttgc aatatttagg ttatcaaatt ctgacatttc
     9001 tattacacca gattcttctt ttgaatacga tattttgact ttatcaccaa cctgagtcaa
     9061 tggcaattcg ctagatactt ttgaagtagc cctaaatatt atatctttat ttgaatctaa
     9121 agttacataa taattagtgt taccacctcg cacatctggg cttattcttg tcactgtgcc
     9181 ttctaatacc tcatctttta aatcattatc aagttttaaa tcattaccct tagattctaa
     9241 tgcatcctta tagtttctta atgcttcatt cttatcttca cctattccaa gtacattata
     9301 atcttcaaca gatacaaatg caactctctt aacaagacca gccttatctt ttaatgatga
     9361 tacatatgtt ggctgaccca atatattata cattacaggg aacgtcgctt cataattttt
     9421 ttcttgaaca ttaccttcag ctgatttcat tgcagccgtt tcagtagctc ctggttgttt
     9481 atataatcta gcttcttttg ttctagaatc aactaacata aatccaattg tagattcatc
     9541 tccacctgaa gatgttatac ctgtatacca ataagacctg ttatcattgc cataaactag
     9601 tgatgtacct tctgttggta ctaaaactcc cttttctgat ataacagagt ttaaaaatcc
     9661 attaacataa acaccccagt ctttaatttg gtctgttaca aagctttgtg gttgtattct
     9721 atccacccat ttaggcgtgt ttttaacatc ataaacattt atttttccag tttctgcatc
     9781 aacagtggct attcctgttg catttgctcc tccatagcca attttatgtt catataaact
     9841 tacaacccaa taaggtcttc cttcatcatt tatttcaaga gtgaaatctg tcataccaac
     9901 atttacaata ccatgtatgt ataaatgtct ttgtaaatcc tgatgtaagt aggcttctgg
     9961 ttggtaaact atctttattg gctttccatc tatttcttga actaattgga cgtcttgagg
    10021 attactagct gatactttta cataaccact tgtaccactt agagatgtta tccatttaat
    10081 tatgtctctg tgaacaagag gcgctaccca gtacaactct ccatctacat tttgtatatg
    10141 aaattttcct aatttggata cactccctat tgcaggaact tctccaagct ttttgtcacc
    10201 taatttcatt gccatatctt catctacaag acgaatgtca tttacactga cagggctaat
    10261 gtcatcagta aatttacttt cagttacttt tcccaatagt tctctatatg attttgcatg
    10321 aagtacaggt gtagaagtta taaagggaac tactacaata tacacaagca aagctattgc
    10381 tattgaaaaa ttatatttag ccattttaga tgctctttca tttctatcta acatcatatc
    10441 tagtattcct gctacaacaa agaaaattat tcctactgaa aataaagatg taaaatctaa
    10501 tcttagtaca ggtaatttaa taaatgcata aattattaca attattaatg caactccata
    10561 tgttttaaga tatcgtttca tttaatactc ctctctaaaa aatgttatta ttactattat
    10621 atccatatat ttacaaaaaa taaatattta tcatatttat tatatattaa gatatcttta
    10681 tatatttata gttctgctta ttcaactata tttaacatta tacgtgtatg tataaatact
    10741 ttttatacac tgtttgtact atccatcccg cacctaaagg agtgggattt ttgcactatt
    10801 ctgtattata tatatccaaa aatattataa aaatacaaat attagcagaa atttaaaaac
    10861 tgaagaaagt attctaacta agatttacaa attcttaatc ctatcgaagt taaactacta
    10921 tcttcagcct aattatctag caatattttt ttatatgaaa aactctaaat caagttttta
    10981 aatgtttgcg atttttgata cataagaatg ttgtctagaa ttatattctg ataataaacc
    11041 ttcatactct aaggttaagc cagataaatt ataaaaatca tttggcgtta cttcaaatgc
    11101 ttgtttattt aaaatgtcta tctcactttt ttctaacatg tcagcaacaa aatttgaaca
    11161 tacatattta tattcagatt gtctctgaat attcattagt aaatatatta atgccattgt
    11221 atcgtaatag taatcttctc tataatcact aattaatttt atatttttat atagattttc
    11281 atattgtaaa ttatctactt ctaacgaata taccctacac acagtgtttt ttcttatagc
    11341 atataatcct tgatttatat tttcttcaac aaatcctcca ataaatggat tccttggatt
    11401 tagccttcca aaagaataca ttggttttag attttcattt aatgatattg acacatgtgt
    11461 atacagcttt ttacttatat tccttattaa gtaagataat atagttcctg tatatgttgt
    11521 tacaatatag actttattca tcatttcccc ccaatctagt aactaaataa tattatacac
    11581 ttacaattta atatcataag atattctaac ataataaatg tggttttaca aattttgatt
    11641 ttaaggtact ttaatattat ataatataat aaaaatataa atataagttt tctatatttt
    11701 taaccaacag atgttttctt caaattaatc agatgattta gtatatcttg aacctttcca
    11761 gagtccttta aatatcctaa tgccccattt atttgtgatt ttaaatctat ttctacttcc
    11821 atatccaaat caacatttcc tttttttata gatatatttt taagtgttat tggataaaga
    11881 taactttttt ccaaagtaat cttatttcca ttaacctcaa atggaacttt tttattgtag
    11941 ttacttaata ttttttctag cattttatca cttagtttaa attttccaat tttcacattc
    12001 tcaagactta caactaagtt tccatccttt aaagtaggtc tcaattcaag ttcatattga
    12061 ctatttacca aaccaaatac cttatatggt cctgctacct ttattttacc atttttcatt
    12121 tcaacaaaat tattttcaag ttcattaatt ccatgcttct gcatcaatgt atatattaaa
    12181 tttttaaact cttcctctga aacagatatt tttccaacca ttctaagtgg attttttact
    12241 acctccatag aatttatata tgagcctttt gataaatatt cttcatctat gattttatta
    12301 ttttgtgaac taacatcata tctttctttt ggtgtcaaga tatatacaac aattcctaca
    12361 agtactaaaa caataacaat taaacttaac aaaatctttg caacctttcc cataatcgtc
    12421 tcctttacaa atctgtcatc agatttaaaa taaatttaga taactaactt cacataatat
    12481 tcttaacaat atacatacta taatacagtc ttttaaaaat taattcaacc aatctaatgt
    12541 tttgtaaata aactgtaaaa acatgttttt agtataaaag ttaatatttt gcataatact
    12601 cttaattaca cctttatttt tttctaatct tggattaaaa ttaatattat gcaatacagt
    12661 gtagtgttct gtattataat caaattaaat aggaggtata gaatatgtca aatgaaacga
    12721 actcatctaa ctttataaag aatattatta taaacgactt agaaacagga aaacatgaca
    12781 gtatcataac tcgttttcca cctgagccaa acggatattt acatattggt catgcaaaaa
    12841 gtatatgtct taattttgga ttagctaagg agtttaatgg aaaagctaat ttaagatttg
    12901 atgatacaaa cccattaaaa gaggatgttg aatatgtaga gtccataaaa gaagatgtaa
    12961 agtggttagg atttgattgg aacgagttaa attttgcatc taactacttt gatgaaatgt
    13021 ataagagagc tttaatacta ataaaaaagg gtaaagctta tgtatgtgat ttgacacaag
    13081 aggaaatgag agaatatcgt ggtactctaa ctgaaccagg aaaagaaagt cctcatagaa
    13141 acagaactat tgaagaaaac cttgatttat ttgaaagaat gaaaaatggt gaatttaaag
    13201 atggtgaaaa aacattaaga gccaaaatag atatgtcttc tccaaatata aaccttagag
    13261 acccaattat atatagaata tctcattcaa cacaccataa tacaggagat aaatggtgca
    13321 tatatcctat gtatgctttt gctcatccaa ttgaagatgc aattgaagga ataactcact
    13381 ctatatgtac tttagagttt gaagaccaaa gacctcttta tgattggttt gttaaagaat
    13441 gtgagatgga aaatatacct agacaaatag aatttgctag acttaatata aataatactg
    13501 tcatgagtaa aaggaaatta aaacaacttg tagatgaggg tattgttgat ggttgggatg
    13561 accctcgtgt tcctacaata tccggaatca gacgtaaggg atatactgca gaagcattgc
    13621 gcaatttctg tagtgaaata ggggtatcaa aagttaactc tacggtagat agtcaaatgc
    13681 ttgattattt cctaagagaa aacttacaac caaaggctcc tcttactatg ggagttttaa
    13741 gacctttaaa acttattata actaattacc cagaagataa gattgagatg cttgaaatag
    13801 agaataatgc taaagatgaa tctcaaggaa agagattagt tcctttctct agagaattgt
    13861 atatagaaca agatgatttt atggaagaac ctgttaagaa atattttaga tttttcccag
    13921 gaaatgaagt ccgtttaaaa ggtgcttatt ttgtaaaatg tactgatgta attaaggatg
    13981 aaaatggaaa tgtagttgaa atacatggta cttatgaccc tgaaactaag agtggctctg
    14041 gctttacagg acgtaaggta aaatctacaa tacattgggt tgatgctaaa tcagctatcc
    14101 catgtgaatt tagattattt gaaccattaa ttcttgatga tatacctgaa aatgaaggta
    14161 aacatttctt agaacaaata aatcctaatt cattggaaat attacaaggt tttgttgaac
    14221 ctacacagat aaaagatgct aaaccttttg ataaattcca atttgttaga aatggattct
    14281 ttagtataga taataaatat acaactgatg aaaaatttgt atttaataga atagttcctc
    14341 taaaaagttc ttttaaacca ggtaaataat cttacaatca aaaaatgaag acttaaaata
    14401 taaattactt ctactataaa taaacttagt tagaagtaat tttttatttg agtcttcatc
    14461 atttacttta aatgattaac ttttttcact acagattccc atgacttcta ttccatcttt
    14521 tatgcattct atgttaagag gaaaatctgg tcctttttct ccgtcaatat ctgttataag
    14581 tgaatcttca cattctatat gcaccttttt agttttaaag tatagtattt catttccatt
    14641 atattgatct aaataatcac ctctaagcac acttatcaaa actggtattg attttggtat
    14701 tggcattcct ttaaaaataa ttacatccaa atatccatca tctatttcag ccttatatgc
    14761 tagatttata tttcctgctg ttttgccatt aaaaatcaac ataagataca tatctccatc
    14821 ataatacatt tcctcagaag atacttttat cttaaatttc ctcatataca atgcttcttc
    14881 tattcccttt atataataag aaattcttcc aaatgaattt tttaagtctg tgtttatcct
    14941 ttgtgataca tccgtaaaca taccagcact tgcaacattt ataaagtact tattattgac
    15001 tttccctatg tctattttct ttggttttga atttatgatt tcatttatag cttcttttat
    15061 atcaaatgaa atttgaagag catttgaaaa atcatttgca gttcctgtag gcaaaattcc
    15121 aattggaatt tttatgtcta attctttcat tgcatttact actaaatcta cagtaccatc
    15181 accaccagct atcaatatgt gttcataaga actatcaact tccttaaatg cattctttac
    15241 aggttcttta atagttaatc tatagggtat caataaataa tttttttctt gatataattc
    15301 tatgatagtg tctaaatttg ataatatctt tttttcacct gaattaggat tgtatataag
    15361 ttttactttt ttcattattt ctccttactc attttattct tttagtatcc aaaatcttca
    15421 tttaaaaaaa tcacatgtat tctttctggt gttaattgtc tttttataag tgtataacta
    15481 cagcatattc ttcctggact tgaacagtct atacatatac cagtagtctt acatccagta
    15541 tttgtactta atcttttaca attggctgga gcagctattg tttttgttct ttttatagct
    15601 tcatctacat ctttaactat tttatttact cctgctataa ctattacttt atcaggtcca
    15661 tatatcatag cagcaaccct attcccattc ccatctaaat tgtaaagttc tccatcctct
    15721 gttatcgcat tagtacttgt aaaataagca tctgcagaaa atgactttct atatatttct
    15781 ttcatttctt ctggtttgat atcttttgca tatctatcaa gaaaattata tttttctctt
    15841 ataaaatcaa tcactcctgt ttcaaataag gtcatagaac ctccacatcc tactagagaa
    15901 cctgctttaa ctatttcttc aattttttta agaagctctt ctttgttact tactaaatac
    15961 cctttcatat tattgttttc caaagatttt attgtttttt ctattctttt ttcattaagc
    16021 caatttaaat tttgatccat tttaacttcc cccttatttt taatatgtac ttaattattt
    16081 ataaacttta ttttctttat aagtttattt tagtacatct aaaaaaaaat acaatataaa
    16141 acttaaatag ttttacataa ttttttccaa aataacttac tcaatcaaaa aaggaaagta
    16201 gatgtactat tctactttcc ctatattgta tgtagtatta tatattgcct aaaatctata
    16261 tattaattat atgtttcata ttatatgaat atcacagtta attaaaatgt aatactatct
    16321 tctttttaaa catataaatt gtattattaa atttaatata tccaacttca ataaatctta
    16381 ttaatttgat gaagaagatg ctatagctgc acttgttgct gccattacac atgcaatttg
    16441 aatagcaaca acaatattta aaaatatatt attagttata gagcctatat tattctcatt
    16501 tcgtatcaaa tctgcataaa ttgaagctac aatagcagaa ctaatcatta ctctattact
    16561 ttttcctaaa gaccaatttc caaagccttt ttcttcttta agtttatttg aaacattttt
    16621 tatctcttct attatttcat cttctctata atctaataat gaaattgctc ctattattgg
    16681 atatccataa taattcattt tacaatcatt ttctttaaat gcttttttaa gttcaatagt
    16741 ctttttacat ttgagcatag ttctatcatc actaaataaa agaatttggg ataatgcttg
    16801 taaatcattt cctttataaa aaccattttc atttagaaat tcataacatt tttctatgta
    16861 ttctaaatct tcatctaaat tttttgaatg aattgcaata agtgctatat tacaatagtc
    16921 atcactagaa gttagaaatg gatgtttttt cctcatgtaa tcatatatta ttttcatttt
    16981 tgatatacat gtaagataat ccattttctc tctattttca taaactattg atgctgtaag
    17041 tggtaaatat gtatcgcccc aaaacttttc acttttaagt tctttgtata tatctaaaat
    17101 catcttaaaa ctactttctg gatttgattc aaacgaaagt agtgttgcca tataaaacat
    17161 attatatcct ctaaaattag agaataatcc agtatttttt ttaataagct ctattcgttc
    17221 ttccactaat ctaggactta ttctcttgtt cttaagggta aatgcaagtg cacaactatg
    17281 ttgtatcaat cccatttgcc aagaaccttt aacttcattt aatgctttgt aattttctat
    17341 taataagttt acttttccat ttataaacga ttgttccata ttgccctcct agtaatatat
    17401 ctcattgtac taatagtttt atatttatta tatatatatt aaatactaca atcaatacat
    17461 attacttcaa agaacatcat tcttcaacaa taatgtagcc ttttttgtaa tgtcttccca
    17521 tattattgac tctatcatag tactctgatt cactttcatt actccatttg ggcacaaata
    17581 catctattgc attagaatta ctaaaatatt tactagaccc cctatccata acagttttta
    17641 attttccatc tacaataatc ttagttccaa aacttaagtg attgtttgct acaaaaccat
    17701 cttctaaatc atctcctgta gcagttatcg ctccatatcc attttcttga ttaagacttg
    17761 tgtaataact aacttcaaaa gttataggta ctaacttctt tattttaata cctaactgat
    17821 tttgtagttt ctccttttgt actttcaact tatactcatc actattttta taatcttcca
    17881 taccttgatt atacttggaa ttaaagcttt tacctgactt tactatagta tctatattta
    17941 ctatagattc tttacaatga tctgcatcta tttttgtttc tccatttacc aataatggag
    18001 ttaaacatac actacataca acacatgtaa tcaatgcttc tttaagcttc ataaattccc
    18061 cccttattat tttgatatat tagcatattt aatattatat gtctacattt agatttaaat
    18121 accacaccca tttgctttaa gaagttgtct tatatttaat tcaccttaaa cacttataaa
    18181 tcctctatct tagaatcata tagaataaag cttttatctt taaatatttt ttattaatct
    18241 tttaaaatct aaaactactt tatctaattt tctctttttt agaatttaag aattaaaatt
    18301 ctataatttt aaaacatatt gaatttattt ttttgagatg atataatttt cttaagaggt
    18361 ggttgatatg ataaaaaata caaaacaaat tacagtagaa gttaatgaaa ttttattaca
    18421 agaaatgtct aagataacta atgacgaaaa taatattttg acagatttga taaatgaatc
    18481 aattgtaaat tatattcttg aaaacaaaaa acataatatt gaaaataaag atttagaaga
    18541 agtgattaaa ttttacaaaa aagaaaatac tgaaaaacct aaaagtgcag caagagctat
    18601 agctgaagca aaaactcata ctctgtatta atcaaataaa aaagttccta aaaactagga
    18661 acttttttat attaataatt attaatatat ctcaatattt atctcaaatc tatcaatata
    18721 agcatgttta atacaatcta ttgtatactt atcttaccct agaaataatc aaaacaaaaa
    18781 taaaattatg ccctttatat ctaaaacaat taatttacat gattaatata caaaaattct
    18841 gacttttaaa ttcgtataca atatcaaact aaaataatat taatgacaaa cccaatataa
    18901 attaaattca aacaggtttg attttacatc aaacttattg tattaatatt aaataacaag
    18961 acaaaaatat ttataaaaat cacaaatttt acataaaact ttatatttga ttcatttaac
    19021 taattattgt aaaaactatt tattttatga taattaacta caccaaatta ttcatctttt
    19081 ttaatatcct taacccattt ttcaacagag ttcaattctt cttcatttac cccctttttg
    19141 aacaaggatt tcataaaact ctcaatagaa cttccatgca tattatctaa aaaagttttt
    19201 gtttcaaaat gtaaatactc ttcttctcca ataagaactg tataatgtgt atgtctatca
    19261 atttttcttg cacttaagaa ttgcttattt actaatcttg ataaaagtgt tagtgttgta
    19321 gtttgcttcc aattatatag tttttccata gcttcaatga catcttttga tgctactgta
    19381 aaatctgaac tccaaataaa tttcatgact tttaattctg ctggtggtat cttagtaata
    19441 gtcatatgat tttacactcc tctcaaactt ctacataatt aaatactata ttaatagact
    19501 tttcgagtca aaggttttat ttaaaatatc tcatccaaat ttattaatac aaaaaattta
    19561 tatttttatt caaaattaaa cacacaatc
//
LOCUS       DAMKMI010000068        19503 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMKMI010000068 DAMKMI010000000
VERSION     DAMKMI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.069"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            540..1739
                     /locus_tag="PC429_002188"
     CDS             540..1739
                     /locus_tag="PC429_002188"
                     /inference="COORDINATES: protein motif:HMM:NF014825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF3847691.1"
                     /translation="MGEIKIFCKHFKFSKSEECPCGSGKTFGQCCYLKEARTFHNENE
                     VLSIIAKNSRKSRINLCLYPECKTKAIGAHAIQENGILSKLAIDKYVWKQNKKENPEL
                     LETMKNEKEPFCFLSKVLIKNATVQTCFCEKHDNKLFEDIEKSDRIFSPNNLKQLFLF
                     TYRTFSFEYYTEIMSNKFYTNMFKDIPQLTKEASIVFNYRESLNKKEEMNYYKSNFDE
                     IIINERYSELATLVIEFPYEFKFANYMSIAPNFDLMGNKLRVYDNKSKMMRRIFVTSF
                     SKDEKSYILISVLKKDFKIYEKYLKSFEQVSQEVIKYYFNAVIPLMSENLIISPELWD
                     EWDDKGKSLVQVAVNDINPRNFLRYLKLILHKLHKKNNSDFSLENCNYNIFGKNNLNA
                     KIKIIKN"
     gene            complement(2115..2516)
                     /locus_tag="PC429_002189"
     CDS             complement(2115..2516)
                     /locus_tag="PC429_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847692.1"
                     /translation="MFAKRLKELRIDAGLKQSELAQKLDISPSTIGMYEQGRRSADQE
                     TLLKISDLFDVSTDYLLGRTNVRNNLFISKNGRDYDAENFKTEKELIENMCLDEDMRK
                     VFNVFSELSEDAREKALKVAELFLLDEKSKK"
     gene            2669..2890
                     /locus_tag="PC429_002190"
     CDS             2669..2890
                     /locus_tag="PC429_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847693.1"
                     /translation="MYNSLVDFRNSKRMTQKEMAKKLGLTLTLYSKVELGIRNPSYNF
                     LMKFKKAFKEVNIDEIFFENKSHEKCMKG"
     gene            2896..3648
                     /locus_tag="PC429_002191"
     CDS             2896..3648
                     /locus_tag="PC429_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDF3847694.1"
                     /translation="MNNLTIIKQNNKYLVESREVAELIEKDHNQLLRSIRGYISVLEQ
                     SAKLHTDDFFIESTYKNENNQKYSCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MERELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQVMKPKADYFDALVERNLLTN
                     IRDTAKELGVKEKTFVLWLIEKKYCYRNLKGKIKPYSNKMQYFELKEFTTPYGHSDTQ
                     TLINPKGREAFRLLLMKDGLIKEKECQITLLG"
     gene            3706..3906
                     /locus_tag="PC429_002192"
     CDS             3706..3906
                     /locus_tag="PC429_002192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847695.1"
                     /translation="MNKEKMAEFYSSKWRINLLIDTQKDLAYSLSDVKGNYPAYLRKE
                     YENLEEAFNKVIAGEGKLLSEL"
     gene            complement(3944..4513)
                     /locus_tag="PC429_002193"
     CDS             complement(3944..4513)
                     /locus_tag="PC429_002193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847696.1"
                     /translation="MLDSNSKKILSFLKPFAIESERIDFSAEQIHENLPSLSLNEIGK
                     AIRFLSENNYLISRKYLGSIYPIVSDVTNKGLNFEEFEPKNQSQNINQTFNIQNMQNS
                     AVGNTGSVTINNGIDFSDLREFIDTNKNLTQAEKYEAQNIVDLIESTSENNIPLKKGF
                     LSRFGDILNKHPDLAMLIAQTLLNRFLIQ"
     gene            4583..4780
                     /locus_tag="PC429_002194"
     CDS             4583..4780
                     /locus_tag="PC429_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847697.1"
                     /translation="MAINDNINEILRDRDLKAWKLAKEIGVDSGNLYAILRGKNKNPT
                     IDTLIKIADYLDVTLDELVGR"
     gene            4933..5214
                     /locus_tag="PC429_002195"
     CDS             4933..5214
                     /locus_tag="PC429_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847698.1"
                     /translation="MPKIEKDLDGTTTITYLEDDIDVPTDDNGYKISMTVLHGNTVCH
                     VVSPETILGRKFTLEEKQEKDEMIYEAIEKMMIDRQLREQKVNKEVANN"
     gene            5434..5616
                     /locus_tag="PC429_002196"
     CDS             5434..5616
                     /locus_tag="PC429_002196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847699.1"
                     /translation="MEVTKRFLEYKIQALSERIEYKKSIGYKCRADEKELGAYEDVLL
                     MLNSEIESIRGLENEK"
     gene            5606..5851
                     /locus_tag="PC429_002197"
     CDS             5606..5851
                     /locus_tag="PC429_002197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847700.1"
                     /translation="MKSRQELIKDIEKYRKAQYLIYLDIVQRAWADRSLTADEQDRIK
                     QEAYAEYKRIEKDTEEAEELLMREEFEMDRPLSVQIM"
     gene            5933..6442
                     /locus_tag="PC429_002198"
     CDS             5933..6442
                     /locus_tag="PC429_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDF3847701.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPGIYKTVEQVVKVDKNSIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            6452..7066
                     /locus_tag="PC429_002199"
     CDS             6452..7066
                     /locus_tag="PC429_002199"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF3847702.1"
                     /translation="MEINNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDSFDAVSGKEESKSNSNNNVKQSNRILTDKQLTRLYAIAN
                     SVGVNKALLKEQVFKQLGKDIKDLNKSEYDAVCAAYEKQAFKGA"
     gene            7843..7941
                     /locus_tag="PC429_002200"
                     /pseudo
     CDS             7843..7941
                     /locus_tag="PC429_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            8011..8262
                     /locus_tag="PC429_002201"
     CDS             8011..8262
                     /locus_tag="PC429_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847703.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIGFNQVYAILENDIRKIGEYESIER
                     AMQVLNDIQKFIENGVRTDYIDSCRVRHNQEKVFEMPFE"
     gene            8280..8636
                     /locus_tag="PC429_002202"
     CDS             8280..8636
                     /locus_tag="PC429_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847704.1"
                     /translation="MAKVWVDAGTFLERTKDIEDMFEFNLRKVRDRNKKINVIDLRVN
                     NEGQGRKGKKVECFNIVTGETKVFNTAVEASKYVYFTDVYIAHLARTGKVSKNGWKAR
                     YIQEVTDGIGKCGTSN"
     gene            8605..8754
                     /locus_tag="PC429_002203"
     CDS             8605..8754
                     /locus_tag="PC429_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847705.1"
                     /translation="MVLANVEQVIKLAEKILNKKKCSVNKAIDIVIKILSKYECEGML
                     RNEFN"
     gene            8741..9190
                     /locus_tag="PC429_002204"
     CDS             8741..9190
                     /locus_tag="PC429_002204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847706.1"
                     /translation="MSLIKYRGYDFENEKWIYSATIMWSNILECLVMLTEGCKWQKVS
                     NVGVCSGEWARNNQEICEGDILKGYDNFHDTSQYGVVKRDIGSIKLYLEWHYLKKLEG
                     EWIELINKTEIYHSRDYKIVGNEYENLEEVRTEFLERKESLENEYLS"
     gene            9293..9469
                     /locus_tag="PC429_002205"
     CDS             9293..9469
                     /locus_tag="PC429_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847707.1"
                     /translation="MDDRLEMINASVNYIQMICESSNIAIIAEQGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
     gene            9579..10652
                     /locus_tag="PC429_002206"
     CDS             9579..10652
                     /locus_tag="PC429_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF3847708.1"
                     /translation="MLTFLDLFAGIGGFRLGMEKAGHKCLGHCEYDKFANLSYNAMHK
                     PKEDEWFERDIREIRTENIPRADVWCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLI
                     RELKEEDRPKYLLIENVKNLLSVNGGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQN
                     RERIFIVGHFRGRSTRKVFPIERKSRKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGG
                     GGAKTGLYFIDLNKNSKVTINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQ
                     NGRRIKESGETMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDS
                     QLYKQAGNAVTANVVYEIAKRMG"
     gene            10733..10942
                     /locus_tag="PC429_002207"
     CDS             10733..10942
                     /locus_tag="PC429_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847709.1"
                     /translation="MYEYILRWQIGLSLENRKIHYTYGSKDVLREKAKVLAKDENIVL
                     ITIDKVDEVIKNTISEKIIERFENL"
     gene            10954..11949
                     /locus_tag="PC429_002208"
     CDS             10954..11949
                     /locus_tag="PC429_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF3847710.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVNQDIEAFFNKKISKVSG
                     DNDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNNSYTHSISFEEDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLKVLDKDAEKEEANSKFVTEFLNATKVKDDKYKTKVFKALVDAFIANLY
                     SDMKQGEDVRSILLYMLREKQKLDINEFADKAIKDDLKDSFKDHVEEKGIESFNIDKK
                     WVEKNLKNRHIKTDTGFEIKGKMDDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK"
     gene            11958..12827
                     /locus_tag="PC429_002209"
     CDS             11958..12827
                     /locus_tag="PC429_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016227206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3847711.1"
                     /translation="MRIGLIDVDSHNFPNLALMKISAYHKQQGDKVEFVNFFEKYDKV
                     YKTKVFTFSDDDYTVINAKEVIKGGTGYDLQNKLPSQIEFMYPDYDLYDIKNIAYGYL
                     TRGCPRKCSFCIVSEKEGSKSYKVANLNQFWKGQKEIKLLDPNILACSKWEELLKQLI
                     DSKAWVDFTQGLDIRIMTEKKAEMINKIKIKRIHFAWDNYEFNTYNKLKEFRSKLNFK
                     KQKLGVYVLTNFNTTFEQDLERIYKLKELEYDPYVMIFEKWKCHHEYRRLQRWVNNKI
                     IFRSVDRFEDYKG"
     gene            12832..13551
                     /locus_tag="PC429_002210"
     CDS             12832..13551
                     /locus_tag="PC429_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HDF3847712.1"
                     /translation="MKHIASFSGGKDSAAMLLLILEKKLPLDEIVFIDTGLEFKEIYN
                     IIDDFEKRINFKITRIKAEKTFEEYFYTVNQQGKRKGQIWGFPYTLGAWCNSRLKLAP
                     ANKYFNKLGEHKRYIGIAFDEPSRYKRLEKNCIAPLFEEKMTEKDCLKYLKEKGFYYE
                     IHHRFKRTGCYLCPKQSLESLRTLRKYYPDLWGSMLKLDKDSPVPFKADGTTVHDLEK
                     RFRNEDIENERQISFFSKGVV"
     gene            13554..13946
                     /locus_tag="PC429_002211"
     CDS             13554..13946
                     /locus_tag="PC429_002211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847713.1"
                     /translation="MDLPDNVIEIDILRINRNNDKKCKCKNREFIVDTVNKSVHCSEC
                     GFRVEPYDALYELAMNFERVNGQVNTLLKQRKEIASYKPHLIVFKRLESKYKGKKMLP
                     VCPICGEAFYFENLTSWINKDFYERRKK"
     gene            14026..14211
                     /locus_tag="PC429_002212"
     CDS             14026..14211
                     /locus_tag="PC429_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847714.1"
                     /translation="MVITEFKVTWQTTREWEKGNIYSSIFTDLEEARAFKKDEENMKE
                     NYNVKLLSRAIVEIEID"
     gene            14233..14448
                     /locus_tag="PC429_002213"
     CDS             14233..14448
                     /locus_tag="PC429_002213"
                     /inference="COORDINATES: protein motif:HMM:NF024363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HDF3847715.1"
                     /translation="MIHELQISSSAFKDVIKNKKQIEVEYTTDFKVGDTVILQSFKDK
                     KFTGIEINKEIEYFVKLGNGYVELGIK"
     gene            14462..14827
                     /locus_tag="PC429_002214"
     CDS             14462..14827
                     /locus_tag="PC429_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847716.1"
                     /translation="MDICKDCFHFKHYRGMEIGRCNKKEKVVYDKAKICELYKNRNQN
                     LNIDELLSEIERWSNEKKNSYVICFTEHDSIQLDIVQDINRPNNPEDDTLIEEYVENN
                     YGYVKYEVIDLGTVEKVEL"
     gene            14844..15287
                     /locus_tag="PC429_002215"
     CDS             14844..15287
                     /locus_tag="PC429_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF3847717.1"
                     /translation="MKVNFTIDGEPKAKARPRMSTANGKAYTPDQTILYENWIRLMYN
                     STVKHFFEGNVKMTVICYFDITKKDREALQKNKVNTKAYKDAIGKVEGLIRPSKKPDL
                     DNIIKSVADSLNGIAYKDDSQIIEVVSKKYYSDKPRVEVELEDVI"
     gene            15388..16224
                     /locus_tag="PC429_002216"
     CDS             15388..16224
                     /locus_tag="PC429_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDF3847718.1"
                     /translation="MNENINREITVLGTLEIEGMKFHDIEGGFGEDKKSMSVKDIAKI
                     HNRELKHVNELINNNIKRFKDGIDILDIKVSRSERPSYLKSLSLEMGYSNQSYANANN
                     IYLLSERGYSKLLKILEDDKAWEQYEKIVDGYFSMRKELNNPLLSASKELQAIFMLDK
                     KQEVLETKIENVNEKLENFMDDAPLFNVECECIVKEVKRVATKSLGGHGSKAYKNKSL
                     RGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVNNYKLPIVFEEEIRLLNSQLSI
                     VS"
     gene            16314..16802
                     /locus_tag="PC429_002217"
     CDS             16314..16802
                     /locus_tag="PC429_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847719.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSSVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKIMDMLYPRIKENELPLF
                     KN"
     gene            17374..17613
                     /locus_tag="PC429_002218"
     CDS             17374..17613
                     /locus_tag="PC429_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847720.1"
                     /translation="MKCPNCGSENIETGILTTGGIYAETIGLKYIGDGKLDKVINKTL
                     PVQSTIYSDLCLDCGEIVRTYIKDDTNKKWCKDKK"
     gene            17883..18731
                     /locus_tag="PC429_002219"
     CDS             17883..18731
                     /locus_tag="PC429_002219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847721.1"
                     /translation="MKFLVDMLKPVFLGFKYIYIKTIGKLIKWILEKEGIAEVIIIAI
                     TIFFLLVIFPPGLKLIMNKFENLLGSTAGEWLSFYGSYLGGILGGIATLMAVVITTNQ
                     TRIIQEENKKENQNINFQNGLTIMSIQNRSEGLTREIQNENNKLQLKNERKDFTNGIA
                     ENMAKYITGINRFFSVKYVRYTNENIKNDYSDTSKKNTNELEKDIEMDKYVPTEMYFL
                     LKIKLKNINLASDLISKLDIVHNYSLNINEEKYRDIELKKFQEDISELINLTEKFITN
                     YLLEEL"
     gene            18781..19473
                     /locus_tag="PC429_002220"
     CDS             18781..19473
                     /locus_tag="PC429_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847722.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISAKYDISLNTLKSWIKRYNWSKE
                     KKKGAPINKRGAPFSNKNSVGYGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKEIKKHESTENGEKAEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
BASE COUNT         8108 a         1986 c         3564 g         5845 t
ORIGIN      
        1 ataatgcata aaaatggaaa aaatatccat tattctacaa aaatagattt ttttattaca
       61 attacgagtt ttttattgca tctatttacc aaaagtatta ttatgtaagt aagataattt
      121 tcaaagacaa ttctgaatag tcggaaaaca tttctaggga tgaaaaggta taattacata
      181 agttttataa aaaatattag ggatttttaa ttaaaaacaa gaacgtaagt tctgatgata
      241 gggggaaagc atgtggaaaa gtcaaagtat aactatattt caaggttaaa ttcaattatg
      301 aaagaattaa aagatttaaa taaggacaaa atcgatgaat ataaagtgaa ggtagaagaa
      361 atatataaaa ataatacaaa ataggattat ttttttatat ataagtgttt aaattgttta
      421 taaaataaat ccgatagcaa gttaagatat agaatttcat ttactctgtt taatttatag
      481 ttttataata gaataaaaat taaaactagt ataacatata attattaagt attgtatgat
      541 tgggggagat taaaatattt tgtaagcatt ttaaattttc taaaagtgaa gaatgtcctt
      601 gtggaagtgg gaaaacattt ggacaatgtt gttacttaaa agaagcaaga acatttcata
      661 atgaaaatga agtattgtca ataatagcaa aaaattcaag aaaaagtaga attaatcttt
      721 gtttatatcc agaatgtaaa actaaggcaa taggagcaca tgcaatacaa gaaaatggta
      781 tattatcaaa attagctata gataaatatg tctggaaaca gaacaaaaaa gaaaatccag
      841 agcttttaga aacaatgaag aatgagaaag agccattttg ttttttatct aaggtgctaa
      901 taaaaaatgc cacagtgcaa acttgttttt gtgaaaaaca tgataataaa ttatttgaag
      961 atatagaaaa atcagatagg attttttcgc ctaataatct aaaacagtta tttcttttta
     1021 catatagaac attttctttt gaatactata cagaaataat gtcaaacaag ttttatacaa
     1081 atatgtttaa agatatacct caattgacaa aagaagcatc aattgtattt aattatagag
     1141 agagtctaaa taaaaaggaa gaaatgaatt attacaagag taattttgat gaaatcatta
     1201 tcaatgaaag atattctgaa ttagctacat tggtaattga attcccttat gagtttaaat
     1261 ttgccaatta tatgagtata gctcctaatt ttgatttaat gggaaataaa cttagggtat
     1321 atgataataa atctaagatg atgagaagaa tatttgtaac ctcattttca aaagatgaaa
     1381 aatcatatat tttaattagc gtattgaaaa aggattttaa aatatatgaa aaatatttaa
     1441 aatcatttga acaagtttct caagaagtta ttaaatatta ttttaatgct gtaatccctt
     1501 taatgtcaga aaatttaata attagcccag aattatggga tgaatgggat gataagggaa
     1561 aaagtcttgt tcaagtagct gtaaatgaca ttaaccccag aaatttttta agatatttga
     1621 aattgatatt acataaacta cataaaaaga ataactcaga cttttcatta gaaaattgta
     1681 attataatat atttggtaaa aataatttaa atgctaaaat taaaattata aagaattaaa
     1741 ttaactttta aacataaata aaacgttttt taatgctaaa caacgcttta tttatgtttt
     1801 tattaagatt tttagttttg agataattta cctaataaag taacgctatt atttttaagt
     1861 tttttatttt cttcttttag actttataga attttttaaa cgattctatt tccatatcct
     1921 ttgaagtttt tgcaaaagtt tatttttgtg aaattactct catttctata gtctgttgac
     1981 ctatggtttt attaatcagt tatatatatt tatctttata ggtatataaa aatgctttat
     2041 ttatgcccac tctatgctat attttaggat ttgaaacagt cctatttaaa taacattaat
     2101 ttctctaaag gaagttattt tttacttttt tcatccaata aaaataattc tgctaccttt
     2161 aacgcttttt ctcttgcatc ttcgctgagt tcgctaaata cattaaaaac ttttctcata
     2221 tcctcatcaa gacacatatt ttcaataagt tctttttccg ttttaaagtt ttcagcatcg
     2281 taatctctgc catttttact tataaacaaa ttgtttctta cgtttgttct tcctagcaaa
     2341 taatctgtag atacatcaaa taaatctgat atttttaata aagtttcttg gtcagcgctt
     2401 cttcgacctt gttcatacat acctattgtg ctaggtgata tatccagctt ttgggctaat
     2461 tcagactgtt ttaatccagc atctattcta agctctttta atctttttgc aaacatctcg
     2521 aaaactcctt tttgatttat aataacacaa aaagtgtaga aaaagatatt actacacgaa
     2581 aagtataaaa aaatattgac aacacgaaat gtgtgatgta ttatataaat atagacacac
     2641 gaaacgtgtg aaaatagggg gtgattaaat gtataatagc ttagtagatt ttagaaattc
     2701 taaaagaatg acccaaaaag aaatggcgaa aaaattaggt ctaacactaa ctctatactc
     2761 aaaagtagag ttaggaatta gaaatccaag ttataacttt cttatgaaat ttaaaaaagc
     2821 tttcaaagaa gtaaatatag atgaaatttt ttttgaaaac aaatcacacg aaaagtgtat
     2881 gaaagggtga taagtatgaa caacttaaca attatcaagc aaaacaataa atacttagtt
     2941 gagagtagag aggtagcaga attaatagaa aaagaccata accaattatt aagaagtata
     3001 agaggatata taagtgtatt agagcagagt gcaaaattgc acactgatga tttttttatt
     3061 gaaagcactt ataagaatga aaataatcag aaatattctt gttatctatt gactaaaaaa
     3121 ggttgtgata tggtagctaa taaaatgaca ggagaaaaag gaatcatatt tacagcgatt
     3181 tatgtaacta agtttgaaga aatggagcga gagttaaaag aacagcaacc taaattacca
     3241 actacgtaca aagaagcatt gcaacagtta ttaatagaag ttgaggaaaa agaacaacta
     3301 caattagaaa atcaagtaat gaaaccaaaa gcagactatt ttgatgcttt agtagaaaga
     3361 aatttactaa caaatataag agatacagca aaagaacttg gagtcaaaga aaaaacattt
     3421 gttttatggt taatagagaa gaaatattgt tataggaatt taaaagggaa aataaaaccc
     3481 tattctaata agatgcagta ttttgaactg aaagaattta caacaccata tggacattca
     3541 gatactcaaa cattaataaa tccaaaagga agagaagcat ttagattatt acttatgaag
     3601 gatggattga taaaagaaaa agaatgtcaa ataactttat taggttaaag attaatagca
     3661 ccttgaaaac taaatacaga atattttgaa aaggaagtga ttaaattgaa taaagaaaaa
     3721 atggcagaat tttattcttc aaaatggaga ataaaccttc ttatagatac acaaaaagat
     3781 ttagcatatt cactatctga tgtaaaaggt aattatccag cttatctaag aaaagaatat
     3841 gaaaacttag aagaagcatt taataaagta attgctggag aaggcaaact cttatcagaa
     3901 ttataaaaaa gtagttagac atatatctaa ctacaaaata aatttactga attaagaagc
     3961 gattgagaag tgtttgagcg ataagcattg ccaaatcagg gtgtttattc agaatatcac
     4021 caaatctaga taaaaaacct ttttttaaag gaatattatt ttcagatgtt gattcaatta
     4081 aatcaactat attttgagct tcatattttt ctgcttgagt aaggttttta ttagtatcta
     4141 taaattctct caaatcagag aaatcaatac cattatttat agtcacagag ccagtattac
     4201 caacagcaga gttttgcata ttttgaatat taaaagtttg atttatattt tgtgattgat
     4261 tttttggttc aaattcttcg aaatttaatc ctttgtttgt aacatccgat acaattgggt
     4321 atattgagcc tagatatttt cttgaaatta aataattatt ttcagacaga aatcttatgg
     4381 cttttccaat ttcatttaaa gataatgatg gtaaattttc atgtatttgt tctgcactaa
     4441 aatcaatgcg ttcgctttca atagcaaaag gttttaaaaa agagagtatt ttctttgaat
     4501 tactgtctaa catgcgtgca ccacctaacc atatatttat aggatttatc ctacaaatat
     4561 agtatatcaa aggaggagaa taatggcaat taatgacaac ataaatgaaa ttttaagaga
     4621 tagagattta aaagcatgga aattagcaaa agaaataggc gtagattcag ggaatttata
     4681 tgcaatttta agaggaaaaa ataaaaatcc aactatagat acattaataa aaatagctga
     4741 ctatttagat gttacattag atgaactagt tggaagatag gagtggtaaa aataaagaat
     4801 aaacattatt aattatgttc taattttaac tttatatttt ttaggaattg tattcagtaa
     4861 gttatttata aatactaatt gagaatagga caatttttaa gatacataaa atataaaaag
     4921 gagggggtta gattgccaaa gatagaaaaa gatttagatg gtacaactac cataacttac
     4981 cttgaagatg atatagatgt tccaacagat gataatggtt ataaaatatc catgacagta
     5041 ttacacggaa atactgtttg ccatgtagta tctcctgaaa caatattagg aagaaagttt
     5101 acattagaag aaaaacaaga aaaagatgaa atgatatatg aagctattga aaagatgatg
     5161 atagatagac agttaagaga gcagaaagta aacaaggaag ttgctaataa ttagcacttc
     5221 ttgcaaagta aaataggaca agtattttaa aacaactagg tatatattac ttaagtacaa
     5281 atataagaag gagggactta gttgagtata agatggtttc taagattttg taaaaaacat
     5341 aagaaaatgc ctacgccacg attttatgca gagtgtgtag cttatatgga agaatgcaga
     5401 caaagaggac ttgaacttta agggggattg aatatggaag taaccaaaag atttttagaa
     5461 tacaaaatac aggctctaag cgagaggata gaatataaaa agtcaattgg atataagtgt
     5521 agagcagacg aaaaagaatt aggagcatat gaggatgttt tacttatgct aaattcagag
     5581 attgaatcaa taaggggatt ggaaaatgaa aagtagacaa gaattaatca aagatataga
     5641 aaaatacaga aaagcacaat acttaatata tttagatata gtacaaagag catgggcaga
     5701 tagaagtctt acagcagatg aacaagacag aattaaacaa gaagcatatg cagagtacaa
     5761 aaggatagaa aaagatacag aagaagcaga agaactgcta atgagagaag aatttgaaat
     5821 ggatagacca ttaagtgttc aaataatgta gaaaagagcc actgcaatgg ctctaatcaa
     5881 aaatatatca aaaatttaat tagctatatt atagcataaa cggagggaaa ttatgagtac
     5941 tttatatgaa ttaactacag atttattaga aatagaagaa ggtttaacag aaacaacagg
     6001 aaatgaagct gaaaaactag aggaaataaa agaaataata aaacaagaga tacaaaataa
     6061 aaacactagg atagtttcag taatattaaa cattgacagt gatataaact ctatagattc
     6121 agagattaaa agattgcaag agttgaaaag ggtcaaaaag aatactcttg atagattaaa
     6181 aagcaatata aaagactgta tggaattact tggtactaaa aaagtagaaa cagttttggg
     6241 aaatataagt ataagaaagt cagcaggtag cttagtcata gaagatgaag aaaagatacc
     6301 tggtatatat aaaacagtag agcaagttgt aaaagtagat aaaaatagta ttaaagactt
     6361 tatcaaaaaa ggtcatgagg ttgaaggttg caggattgaa tatggaacta cactaacaat
     6421 tccaaaagct aaaaaagagt aggtgaggac catggaaatt aataatattt acattaaatt
     6481 aatggatgtt agagttaaat ttagtaagtt gaatttaaag aaaagtggag aaaataagtt
     6541 cgctaacttc aagtattttg agttagcaga ctttctacca caagcaactg gattacttga
     6601 agaagctaag ctatgcccta tagtgacctt tacaaatgaa tatgcaactc taacattaat
     6661 taatggagaa aacccatcag aacagattgt atttacttct cccatgagag atttacaact
     6721 taaaggttct aatgaattac aggcactagg aggtatagaa acctatcaaa ctagatattt
     6781 gtatattcag ttactaaata taactgaaaa tgatagtttt gacgcagtaa gtggaaaaga
     6841 ggaatctaaa agcaattcta ataacaatgt taaacaaagt aacagaatat tgactgataa
     6901 acaactaact agattatatg caatagctaa tagtgttgga gttaataaag cattattaaa
     6961 agaacaggtt tttaaacagc taggaaaaga tataaaggat ttaaataaga gtgaatatga
     7021 tgctgtttgt gcagcatatg agaaacaagc ctttaagggt gcataacagg aggtgataga
     7081 gtggcagttt tcagacagat atatacaagc ttttggactg acccaaaggt acaagaggag
     7141 tggacaccag aagataaatt tttctttata cttcttctta gtaatcctca aacaacacaa
     7201 ataggagttt accaagtaac taaaaaacaa ctagcatttt ggatggggta ctcagaagaa
     7261 agtataagag cattaatgga tagattcgta aatcaccata agtgtattaa atataatccc
     7321 gatactagag aaattgccat aaaaaactgg ggcaaatata acttaactaa aggtggtaaa
     7381 ccaattatag atttacttaa taaagaactc aaagaagtta aagatattga gttaataaaa
     7441 tatgtaattc catcaataga aaaatcagat ataagaaaaa tatttgagga ttattacgaa
     7501 atggctaaaa atggggattc ttacgatacg tcgacgatac gtgaacgagt cgtaccacga
     7561 tacggggaca ataacaataa gaataacaat aagaataaca ataagaataa caacaacaat
     7621 aaggatgatt cggtggttgt ggataaaata aaacaatact ttgatttaga atctaaagac
     7681 attgaaaaaa ttgttgatat attcatacat acaggaagag gaattgacta tttagaggaa
     7741 aaactaaggt tggtaaaaaa tacagatgga gtaaaaagta ttacaggcta tctcataaaa
     7801 gcattagaag aagattataa gcctatacca agtagaaaaa ataagactaa gtttcataat
     7861 tttaacgaga catttactaa gtatacgtct gatgaactag acgagattat taaaaagagt
     7921 caaaaagaga agttcagttg aattaaatag tctagggagt agttatacaa tattacttcc
     7981 taggagttaa aaaatattgg agggatagaa atgataataa ttagaagtca agatagatta
     8041 gatttaatga gagttaatag agttgaaata ggttttaatc aagtgtatgc aatacttgaa
     8101 aatgatatta gaaaaatagg tgaatatgaa agtattgaaa gagctatgca agtgttaaat
     8161 gatatacaga agtttattga aaatggagta agaacagatt atatagattc ttgtagagtt
     8221 agacataatc aagagaaagt atttgaaatg ccatttgaat aaaaaggggg gaactaaata
     8281 tggctaaggt ttgggtggat gcaggaacat ttttagaaag aactaaggat atagaagata
     8341 tgtttgaatt taatctaaga aaagtaagag atagaaataa aaaaataaat gtaattgatt
     8401 tgcgtgtaaa taatgagggg caaggaagaa aaggtaaaaa agtagagtgt tttaatattg
     8461 tgactggaga aactaaagta tttaatactg ctgttgaagc aagtaaatac gtatatttta
     8521 cagatgttta tattgctcat ttagcaagaa caggtaaggt ttctaaaaat ggatggaaag
     8581 ctagatatat tcaagaggtg acagatggta ttggcaaatg tggaacaagt aattaagtta
     8641 gctgaaaaga tattaaataa gaaaaagtgt tctgttaata aagctattga tatagttata
     8701 aaaatattaa gtaaatatga gtgcgagggg atgttaagaa atgagtttaa ttaagtatag
     8761 aggttatgat tttgagaacg aaaagtggat ttattcagca acaataatgt ggagcaatat
     8821 acttgaatgt ttagttatgt tgacagaggg ttgcaaatgg cagaaagtct ctaatgttgg
     8881 ggtatgctct ggagaatggg ctagaaacaa tcaagaaatt tgtgaggggg atatattgaa
     8941 aggatatgat aatttccatg acacaagcca atatggagtt gtaaaaaggg atattggtag
     9001 tattaagtta tatttggaat ggcattattt aaagaaactt gaaggagaat ggatagagct
     9061 tataaataaa acagaaatat atcatagcag agattacaaa atagttggta atgaatatga
     9121 gaacttagag gaagtcagaa cagagttctt agaacgtaag gagagtcttg agaatgaata
     9181 tcttagctag tgcgatatta gtaataggaa gttttatagc tggtagagtt tatgagtata
     9241 gattgaatct aaaagagtgt gaaaattgcg acaataaaag aggtgtataa gaatggatga
     9301 tagattagaa atgataaatg cttctgtaaa ttatatacag atgatatgtg aaagttcaaa
     9361 tatagctatt atagcagagc aaggaagagt tagaatatta gatttagaaa ctaaagaaaa
     9421 atatgattta ttaaaaaata aactcgagga aatgttagaa gaaatataag tgaaaatatc
     9481 taattaagac agtttagaga gttacaaaat atcttttagt ataaattatt gttgaagtgt
     9541 tttgtgactc tcaaaaatga aaataaggag gcgttgtatt gcttacattt ttagatttat
     9601 tcgcagggat aggtggcttt aggctaggga tggaaaaagc aggacataaa tgtttgggac
     9661 attgcgaata tgataaattc gcaaatttaa gttataatgc catgcacaaa ccgaaggagg
     9721 atgaatggtt tgaaagagat attagagaaa ttagaacaga aaatatccca agagcagatg
     9781 tctggtgttt tggattccca tgtcaagaca tttctgttgc agggaaacaa tttggattca
     9841 gaggagaacg ttcaagttta ttttttacag ttacaaaact tattagagaa ctcaaagaag
     9901 aagatagacc caagtattta cttattgaaa acgttaaaaa tctacttagt gttaatggag
     9961 gatttgattt cctcaaagtt ctcgttgaac tggatgaaat cggctatgat gcagagtggc
    10021 aagttcttaa ttctaaaaac ttcggagtac cccaaaatag agaacgaata ttcattgttg
    10081 gacattttag aggacgaagt acacgaaaag tatttcctat cgaaagaaaa agtagaaaaa
    10141 atcttgagca actaaataat ccaactcata gtacaaatag aatttatgat gcagttggaa
    10201 ttgctagatg tattagaagt caggcaggag gtggaggtgc taaaacaggt ctatacttta
    10261 tagacttaaa taaaaactct aaagtaacaa taaatgctag atgccttaaa gcaaaatata
    10321 atgcaggtgt gacaaataga aattgtgata atagtggagt tttagttaat gcagttttaa
    10381 cgcccgatag ggtaaataaa agacaaaatg gtcgtagaat taaagaaagc ggagaaacaa
    10441 tgttcacatt gacagctcaa gataaacatg gaattttgaa aaatggagat ataagaaggt
    10501 taacaccaaa ggaatgcttt aggttgcaag gatttccgga taaatattac gaaagagcag
    10561 caagtgtatg ctcagatagt caactgtaca agcaagcagg aaatgctgtt actgcaaatg
    10621 ttgtatatga aatagcaaaa agaatgggct aaaagttgca aaatgtcttt taatatgaat
    10681 atttttgaag tgttttgtaa ctctcaaaaa tgaaaaataa ggggtgggat aagtgtatga
    10741 atatatactt agatggcaga taggattgtc attagaaaat agaaaaatac attacacata
    10801 tggaagtaaa gacgttttaa gagagaaagc aaaggtattg gctaaagatg aaaatatagt
    10861 actaataact atagataagg tagatgaagt tataaaaaac acgataagcg aaaagattat
    10921 agaacgtttt gaaaatttat aaggggtgga attatgataa tacacaaatt tataatacat
    10981 gttttagata agaatagcga tacgccgata ttgaacgatt ttgagggcag agttaatcaa
    11041 gatatagaag ctttcttcaa caagaaaatc agcaaagtat ctggagataa tgacatcaga
    11101 acagcagtat ttaatgacta tagtaacaat ctaattaaga agtgttgtga acaaattatt
    11161 tatgatgaaa gttcattttt aaataactct aaagagattg cagcttattt atttgatgtt
    11221 atgaagctta atgctacatt agaatcttgc gacttagcaa tttgcttata ctctcaaaaa
    11281 gatgaaaaga aagttgctat attaaagctt gattacaata attcgtatac tcattctatt
    11341 agctttgaag aggataaatt taatatacag atgtctaaga atgaaattaa tatacaagag
    11401 actaagacag tcaaaattgc tgccctagtt ggattaagtg gaatgaatga cgaatatcat
    11461 ctaaaagtat tggataagga tgcagagaag gaagaagcta attctaagtt tgttacagag
    11521 tttttaaatg ctactaaagt gaaagatgat aagtataaga ctaaggtgtt taaagctctt
    11581 gtggatgctt ttatagcaaa tttgtatagt gatatgaagc aaggagaaga tgtaagaagt
    11641 atactacttt acatgctaag agaaaaacaa aaacttgata taaatgagtt tgctgataag
    11701 gcgataaagg atgatttaaa agatagtttt aaggaccatg tggaagaaaa aggaattgaa
    11761 agttttaata ttgataaaaa atgggttgaa aagaatttaa aaaatcgaca tataaaaaca
    11821 gatacaggtt ttgagataaa aggcaagatg gatgattttg aggacccaat gaaatataca
    11881 gtaagacaaa atcaaaatgg gtctatagat atagttatta agaatgtaac attttatgaa
    11941 gaaaagtagg tgctcatatg agaataggat taattgatgt tgatagtcat aactttccta
    12001 acttagcttt aatgaaaata agtgcatacc acaaacaaca aggcgacaaa gtggaatttg
    12061 taaacttctt tgaaaaatat gacaaagtat ataaaactaa agtatttact ttttcagatg
    12121 atgattacac agttataaac gctaaagaag tcataaaggg tggtacagga tatgatttac
    12181 agaataaatt accatcacag attgaattta tgtatcccga ttatgattta tatgatatta
    12241 aaaatattgc ttatggctac ttaacaagag ggtgtcctcg aaaatgttca ttttgtattg
    12301 tttcagaaaa agaaggtagt aaaagctata aagtagccaa cttaaaccag ttttggaagg
    12361 ggcaaaaaga gattaagcta ttagacccta atatcttagc ttgtagtaag tgggaagaac
    12421 ttttaaagca acttatagat agtaaagctt gggttgattt cactcaagga cttgatataa
    12481 ggattatgac agagaaaaaa gcagaaatga ttaacaaaat taaaataaaa cgaatccatt
    12541 ttgcgtggga taattacgaa tttaacacat acaacaagtt aaaagaattt agaagtaaat
    12601 tgaattttaa gaagcaaaaa ttaggtgttt atgttcttac taattttaat actacttttg
    12661 agcaagattt agaacgtatt tacaaattaa aagagctaga atatgaccct tacgtgatga
    12721 tattcgaaaa atggaaatgc catcatgagt atagaaggtt gcaaagatgg gttaataata
    12781 agattatttt tagaagtgta gatagatttg aggattataa ggggtgagaa tatgaaacat
    12841 atcgcaagtt ttagtggagg aaaagattct gctgcaatgc tccttctaat attggaaaag
    12901 aaattacctc ttgatgaaat tgtatttata gatacaggac tagaatttaa agaaatttat
    12961 aacataatag atgattttga aaaaagaata aactttaaaa taacaaggat taaagcagaa
    13021 aaaaccttcg aggaatactt ttatactgtt aatcaacaag gtaagcgtaa agggcaaata
    13081 tggggttttc cgtatactct aggggcatgg tgtaatagca gattaaaact tgctcctgct
    13141 aataaatatt ttaataaact aggagagcat aaaaggtata ttggaattgc ctttgatgaa
    13201 cctagcagat ataaaagact agaaaaaaac tgcatagcac cattgtttga agaaaaaatg
    13261 actgaaaaag attgcttgaa atacttgaaa gaaaaaggtt tctattatga aatacatcac
    13321 agattcaagc gaactggttg ctacttatgt cctaaacaaa gcttagagag cctcagaact
    13381 ttaaggaaat actatcccga tttgtggggg agtatgctta aattagataa agatagtcct
    13441 gttcctttca aagctgatgg aactacagtg catgacttgg aaaagcgatt tagaaatgaa
    13501 gatatagaaa atgaaagaca gataagtttc tttagtaaag gagtggttta gaaatggatt
    13561 taccagataa tgttatcgaa attgatatat taagaattaa tagaaataat gataagaaat
    13621 gtaagtgtaa gaatagagaa tttatagtag atacagttaa taaatcagtt cattgttcag
    13681 aatgtggatt tagagtagag ccatatgatg cgctatatga actagcaatg aactttgaaa
    13741 gagtaaatgg gcaagtaaac acattattaa aacagagaaa agaaatagct agttataaac
    13801 ctcacttgat agtttttaag aggcttgaaa gtaaatacaa gggtaaaaaa atgttaccag
    13861 tttgcccaat ttgtggagaa gcattttatt ttgaaaattt aacttcatgg attaataaag
    13921 atttctatga aagaagaaaa aaataaaata aatagtcaag gtaagtttgt gaatgaaact
    13981 agaatgttat agacttactt tgacttataa aaaggagtgg tcaatatagt tattacagag
    14041 tttaaagtga cttggcagac aactagagaa tgggaaaaag ggaacatata cagctctata
    14101 tttactgatt tagaagaagc aagagctttt aaaaaagatg aggaaaatat gaaagaaaat
    14161 tataatgtga agcttttaag cagagctata gtcgaaattg aaatagatta atttttaaaa
    14221 gggtgtgagt ttatgataca tgaattgcaa atatcttcta gtgcttttaa agatgtaatc
    14281 aagaataaaa aacaaattga agtagagtat acaacagatt ttaaagtagg agatacagtc
    14341 atattgcagt catttaagga taaaaagttt acagggattg agataaacaa agagattgag
    14401 tattttgtta aacttggaaa tgggtatgtt gagttgggaa taaaatagag gaggaataaa
    14461 aatggatata tgtaaagatt gttttcattt taaacattat agaggtatgg aaattggaag
    14521 atgtaataaa aaagaaaaag tggtatatga taaagcaaag atatgtgaac tttataaaaa
    14581 tagaaatcaa aatctgaaca tagatgagct attaagtgaa atagaaaggt ggtcaaatga
    14641 aaaaaagaat agttatgtga tatgttttac tgaacatgac agtatacaac ttgatatagt
    14701 ccaagatatt aatagaccta ataatccaga agatgacaca cttatagaag aatatgttga
    14761 aaataattat ggttatgtaa aatatgaagt cattgattta ggcactgtag agaaagttga
    14821 gttgtaaaac taggaggtca ataatgaagg ttaattttac aatagatgga gaaccaaaag
    14881 caaaggcacg tcctagaatg agtacggcaa atggtaaggc ttatacacct gaccaaacta
    14941 tattatatga aaactggatt agactcatgt ataactctac agtaaagcat ttctttgagg
    15001 gtaatgtgaa aatgacagtt atttgttact ttgacattac taaaaaagat agagaagcac
    15061 tacagaaaaa taaggtaaat actaaagcgt ataaggatgc tatagggaag gtagaagggt
    15121 taataagacc aagcaagaaa cccgatttag acaatataat caagtctgta gctgatagtt
    15181 tgaatggtat agcttacaaa gatgattctc aaattataga agtggtgagt aagaaatatt
    15241 atagtgacaa accaagagtc gaggttgagc tagaagatgt tatttaacca acggaaaatc
    15301 tgttcgttag agtatgtact tagtttttca taaaaatgtg aaaaagctaa ataaagaata
    15361 tatcaaatga taaaggagag atagattatg aatgaaaata taaatagaga aataacagta
    15421 cttggaactt tagaaatcga gggaatgaaa tttcatgata ttgagggtgg atttggagaa
    15481 gataagaaat caatgtcagt aaaggatata gctaagatac ataatagaga acttaaacat
    15541 gtaaatgagc ttataaacaa taatataaaa agatttaaag atggtataga tattttagat
    15601 ataaaagtga gtcgttcaga gcgaccaagt tatttaaaat cacttagctt ggaaatggga
    15661 tactctaatc aatcttatgc aaatgcaaat aacatatatt tgttatctga aagaggttat
    15721 tcaaaactac taaagatatt agaagatgat aaggcttggg aacaatacga gaaaatagtt
    15781 gatggatatt tctctatgag aaaagaacta aataatcctc ttttaagtgc atcaaaggag
    15841 ttacaggcta tatttatgct agataagaaa caagaagtct tagaaactaa aatagagaat
    15901 gttaatgaga aattagagaa ctttatggat gatgcgccac tttttaacgt tgagtgtgag
    15961 tgtattgtta aagaggttaa gagggtagca acaaaatcac taggaggaca tggaagtaag
    16021 gcttataaaa acaaatcttt aagaggtaaa gtatatagtg atatatatca tcagattaaa
    16081 cgagagtttg gagtagatag ttataaggcc ataaagcgtt gtcagttaga taaagtatta
    16141 gagattgtaa acaattataa gttacctata gtgtttgaag aagaaataag acttttgaat
    16201 agtcaactat caatagtaag ttaaaagaaa aaaggagtgc tttcacactc cacttgtcaa
    16261 aaatataaag cttttatcca agattattat aacataaaca ggagtgtgga agtatggata
    16321 ataatatcaa taaaaaagaa ctatttaaaa aagtagaagg tagactacat cattataaat
    16381 ttttaagtgc agaaattaaa aatcttgaat tagatataga aagtagagaa aatgagatat
    16441 ttgggtgtaa ggctgttgga tatgatgaaa aagtaagtcc aacatatgct tttaattcaa
    16501 gtgtggagaa tgagattata aaaaaagaaa gagatattac tagattgaaa aaactgaaaa
    16561 aagataagga aattgaaaag aagaaaatag aaaatgcact tacatgctta gatataagag
    16621 aagaacattt ttttaaactg ttttataata gcagaatgaa aaatagtatg gtttatatat
    16681 ccttagaaat gaactcagat agaaaaacat gtagatgtgt gagggaaaga ttagtgtata
    16741 aaattatgga tatgctttat ccaagaatta aggaaaatga actaccatta tttaaaaatt
    16801 agaaaattcc ccagtttttc cccagaaatt cccactttat tccctacttt ctcccctttt
    16861 tgattaaaaa agcatgagat aatagtattg tggaaataaa gattttcctc tcaaaactaa
    16921 ataattgcta gattagccct ttaaactaac ctagcaatat gaacagacta ggcagggcgt
    16981 gaggacgctg ttagttcaat tctaactatg ttcaaatatt aatcaacgta tacactaaaa
    17041 gtagagaaat tgagggcaaa attttatatt ttgtatctta attcagaagt ctaaaaatcg
    17101 ggtggggctt ggtaacctca ctcaccatgc aagtagtggt gtttaatcta ggttcgattc
    17161 ctagaacttg ctaccaacaa tatacattcc ctaaaaaagg ctaagttacc cctacttagc
    17221 ctttttaaat tctaaaatta ttctaataaa gttcatattc agtacacaaa cttttggtaa
    17281 tatataagca aatcaaagat tgtgtagaca ttaaattaat tcaaatatat atacttgtac
    17341 ataaaatata tgataattct aggaggtata aacatgaaat gtcctaactg tggtagtgaa
    17401 aatatagaaa caggtatttt aacaacagga ggtatatatg ctgaaactat tgggttgaag
    17461 tatataggag atggaaagtt agataaagtt ataaataaaa cgctacctgt acaatctact
    17521 atatattcag atttatgttt agattgtggt gaaatagtta gaacttatat aaaagatgat
    17581 actaataaga aatggtgcaa agataaaaaa tagattaaat tgaaaggtca tcttaataga
    17641 tggtcttttt ttatacaatg aattaaaaat gaaatatatt tgtgtatgat ggataggaat
    17701 gaggcaaaag tattatttag aattattttg taactgtcga atggtttttg aaggatattt
    17761 acctttgaag tagaatttta tactttggag gggatatata tggaagtaaa tgatttagga
    17821 aaaaagataa ttgttttttc aaagctacat taaatttatt tttatttttt attactgaat
    17881 ggatgaaatt tttagttgat atgttaaaac cagttttttt aggatttaaa tatatataca
    17941 ttaagactat aggtaaatta ataaagtgga tattagaaaa agaaggaata gcagaggtaa
    18001 taataattgc tataactata ttttttctac tggttatatt tcctcctgga ctaaaattaa
    18061 taatgaacaa gtttgaaaat ttattaggta gcaccgctgg agaatggtta tctttttatg
    18121 gaagttatct aggtggtatt ttgggaggaa tagcaactct tatggctgtt gttattacaa
    18181 ctaatcaaac aagaataata caggaagaaa ataaaaagga aaatcagaac attaactttc
    18241 aaaatggact tacaatcatg tctatacaaa atcgaagtga aggcttaaca agggaaatac
    18301 aaaatgaaaa taataaattg caacttaaaa atgaaagaaa agattttacg aatggaatag
    18361 cagaaaatat ggctaagtat attacaggta taaatagatt tttttctgta aagtatgtta
    18421 gatacactaa tgaaaatatt aaaaatgatt atagcgatac ttcgaaaaaa aatacaaatg
    18481 agctagaaaa agatattgag atggataaat atgtaccaac agaaatgtat tttttactaa
    18541 agataaaatt gaaaaatata aatctagcaa gtgacttaat ttctaaatta gatattgtac
    18601 ataactattc tctcaatata aatgaagaaa aatatagaga tatagaatta aaaaaatttc
    18661 aagaagatat aagcgaatta attaatttaa ctgaaaaatt cataacgaac tatttattag
    18721 aagaactcta aaaagagttc ttttttattc cccaaaacaa acaaataaag aggtggtgat
    18781 gtgcaagatg tcaaagaaaa ggtaaaacaa gattacttaa aaggaatgaa acaaaaggaa
    18841 atatcagcaa agtatgacat tagtttaaat actttaaagt catggataaa aaggtataat
    18901 tggtcaaagg aaaaaaagaa gggtgcacct ataaataaaa ggggtgcacc cttttctaat
    18961 aaaaattcag ttggatatgg tgctccaaaa gagaataaga atgctgaaaa gtttggtttc
    19021 ttttcaaaat atctacccga agaaactagg gaattgatac aagaaatatc tataaaagat
    19081 aaatttgata ttctttggga gcaaataaca atccagtatg cagcaataat aagagcacaa
    19141 aagataatgt atgttaaaga caaagaagaa atgattaagg aaatcaagaa acatgaaagc
    19201 acagaaaatg gtgagaaggc agaatatgaa tttcaatttg catgggatag gcaagcatct
    19261 tttcttaatg cacagagtag ggcaatgagt gagttaagga gtttaattaa gcagtatgat
    19321 gaaatgattc ataaggattg gaatttggct acagaggagc agaaaacaag agttgagaag
    19381 ttgaaatgtg aagttgataa cctaagtaaa gatgatattg gagatgatga gttgaaaata
    19441 agtgtagatt atggtgatag aaatgatagt tagagtaaat tttaatccag atttcaagga
    19501 agc
//
LOCUS       DAMKMI010000069        19299 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMKMI010000069 DAMKMI010000000
VERSION     DAMKMI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19299
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.070"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            391..903
                     /locus_tag="PC429_002221"
     CDS             391..903
                     /locus_tag="PC429_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847723.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KMDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            complement(1356..2453)
                     /locus_tag="PC429_002222"
     CDS             complement(1356..2453)
                     /locus_tag="PC429_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF3847724.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            2746..4272
                     /gene="glpK"
                     /locus_tag="PC429_002223"
     CDS             2746..4272
                     /gene="glpK"
                     /locus_tag="PC429_002223"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF3847725.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4313..5428
                     /locus_tag="PC429_002224"
     CDS             4313..5428
                     /locus_tag="PC429_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF3847726.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(5709..6473)
                     /locus_tag="PC429_002225"
     CDS             complement(5709..6473)
                     /locus_tag="PC429_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF3847727.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            6732..6822
                     /locus_tag="PC429_002226"
     tRNA            6732..6822
                     /locus_tag="PC429_002226"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6766..6768,aa:Ser,seq:gct)
     gene            6910..7698
                     /locus_tag="PC429_002227"
     CDS             6910..7698
                     /locus_tag="PC429_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF3847728.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLTFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            7719..8459
                     /locus_tag="PC429_002228"
     CDS             7719..8459
                     /locus_tag="PC429_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF3847729.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            8635..9009
                     /locus_tag="PC429_002229"
     CDS             8635..9009
                     /locus_tag="PC429_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847730.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            9101..10561
                     /locus_tag="PC429_002230"
     CDS             9101..10561
                     /locus_tag="PC429_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF3847731.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            10614..13643
                     /locus_tag="PC429_002231"
     CDS             10614..13643
                     /locus_tag="PC429_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3847732.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            13720..15786
                     /locus_tag="PC429_002232"
     CDS             13720..15786
                     /locus_tag="PC429_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF3847733.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    16169..16379
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            16605..17420
                     /locus_tag="PC429_002233"
     CDS             16605..17420
                     /locus_tag="PC429_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF3847734.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            17448..18125
                     /locus_tag="PC429_002234"
     CDS             17448..18125
                     /locus_tag="PC429_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF3847735.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            18184..18906
                     /locus_tag="PC429_002235"
     CDS             18184..18906
                     /locus_tag="PC429_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF3847736.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         7647 a         2019 c         3413 g         6220 t
ORIGIN      
        1 ttatttttag aatattcagt aaaatgattt tgatatactc tctatatttt gtatgttaaa
       61 agtaaataaa ttattgttaa agaaggtaaa gacttttaaa tttcttatca taggatttta
      121 agtaaataag aaggattgga gtaattaaaa atttttattt tgcaaaaata agtatttatt
      181 tatatggata aatttgattt taaatctata aatatagata aaatctaatt atttaacttc
      241 aactattcct caaaaaagca acttttaagg aatagttgaa gcaaaaagtt tggttacata
      301 atagtaattt tagttacaag aatgaaactg taaaacttag gattttcttt atataatttc
      361 attgagaaca tttaatttag gaggtaagat atgaaagtaa aaaaatttaa aagtggtata
      421 gtaatggcat taacagtaat ggcaattgga gcatcattga caaatgtatc agctatggaa
      481 ttaaacaaga gtaaagagtt tgaaactaaa tacaatgaaa tgaagatgga ttttcaagag
      541 gaatatggaa aagaagacaa atttaatata acattggatg atgaatttac aaaagaagaa
      601 gtaaatggag ctaaagggat tataaagaca actaataaaa tgaataatga ggttgtagta
      661 ttaaattatt ttgatgaaat gaaatctgga attgaatttg agttgaagcc aaaagcagaa
      721 aaaaatgcaa ttttgagaga tgaatttcaa caaggttatg gaaaggaaga taaattcaaa
      781 gttgtgttag atgatgaatt tacaaaagaa gaggtaaatg gagctaaggg cattataaag
      841 actacagata aagagacagg tgaggttatc gtatataatt actttgatga gcttgaaaaa
      901 taatgcttaa agattacttt gattgaatga aaaggtaatt gtgttgagaa tataaaatga
      961 ctgaataaaa acagttgtat caaaacaaac aataatagaa tgtaagaatg attgtgttga
     1021 aaatattaaa tgactgaata aaaaacgatt gtgtcaaaaa taaaaaataa ttaagtgtaa
     1081 gaatgattgt atcaagaata taaaatgact gaataaaaaa cgattgcgtc aaaaataaaa
     1141 aacaattaaa tgtaagaata attatgtcaa aaatataaaa taaattaaaa gaaaaagatt
     1201 atattgaagg aaaaagattt gaaatagggg tgaactttac atattatagt tcacccttat
     1261 acaaaaatat aaaatataat ttattatagg aaaatttaaa aaaatcagtc tatttaattt
     1321 atactgatat attgtaggat tttacaaaca aactgttata taactttttt tatctccaat
     1381 tttgatttta agatatctaa gaatttataa caatcttctg gatatatttc tcccatattt
     1441 gctacttgga atatattagc atctaggaat ttaccttttc ctggatagac aacatagcca
     1501 ctttcccttt ctagccctaa tataaactca tttacattga tttctttagg tagaaataca
     1561 gatgttactg tattagacat atacttatct ggaagtaaaa atgttagacc caattcttcc
     1621 ataccattcc ttaaaatagc agaacattct ttgtatcttt ctattttact gtcatcttcc
     1681 aaaaactcag ttaaagcttc attaagggca aatattaaag taacattagg tgtatttggt
     1741 gtttgagaag atgatttagc aactttatag tgtttataaa gattaagata cacattctta
     1801 cattgatttt ctttaatatt ttgtaaaata ctctctttag cacaaaggta tgcagaacct
     1861 ggaaatgctc caagagcttt gccaccaaca cctgttatta tatctatatt aaactcttcc
     1921 acatcaatat attcacctgc tgaggcactt attgcatcta caaaaaatat tttgttatac
     1981 ttcttagcaa gttttcctag ttcagggact ggatttatca ttcctgaact agtttcatgg
     2041 aacaccatag caattacttt tatatcagaa ttttcaatta tgtatttttc taaaatgtct
     2101 aggtccgggt attctgccca ttcaaaatta ggtttatatg tttttacatt ataagtagat
     2161 aatatttctt ctagcctttc tccaaaaaca ccattactaa ctagcagaac tgcatcttct
     2221 ttgtcaaaga tagaagatag tacacattca tttacagatg ttccagagcc agatacaatt
     2281 aatgagtagt aattagaagt tgcattatat agtttattga ttttttgttg tgtatctttg
     2341 aataaatcca taaactcatt tcctctatgg catatatcat agtgaagaag tgaatttctg
     2401 actttatctg taaccattac tggtccagga ctgaatagat atgttttttt cattgtaaag
     2461 cctccttaaa aagttaaatt taaatttttg aatgactgat tatgtgtaaa tattaaaata
     2521 ttatgtgtta ttttaactag attatagaat aaaataataa atattgcaat aatatttcaa
     2581 taaaaatact tttttaatca ctataattaa aaataacaaa aaatattgta atgaatattg
     2641 cataaatcca catgatgata taaaatataa ataacttgaa tagattatat attcaaaaaa
     2701 taaaaaagaa tttattaatt ctgaaaataa agggggacat ttaaagtgaa atatgtatta
     2761 gcaattgacc aaggaacaac aggaacaaga gcagtattga taaacgaaaa atcagagttt
     2821 gtttttagcg attatatgga acatactcaa atatatccac aatctggata tgttgaacat
     2881 gaccctatag aaatttggaa taatacaaaa actgtgagta ataatgtatt acaacaggct
     2941 tttgaaaatg gaatcaatga aagtgatata aaaggaattg gtatagataa tcaaggtgaa
     3001 acagtaatgt tatgggataa aaatacaggt aaaccacttt acaatgctat tgtgtggcag
     3061 tgcaggagaa cttatgatta tgtggaaaat ttaaagaata taccaggttt agaaaataag
     3121 ataatagata agactgggtt aattatagac ccatatttct caggtacaaa aattaaatgg
     3181 gtaatagata atgtaaaagg tgtaaaagaa aaaattaaaa atggagaagt acttgcagga
     3241 acattagata gttggttaat atggaaaatg acaggaggaa aatctttttt aactgatgta
     3301 tctacagcag caagaacaat gctttttaat ataagagata tggattggga tagagagttg
     3361 cttgacatta taggagtacc tagaagtatt ttagcagata taatgcctac gagtggtgat
     3421 tttggaacta cagataagga ggcattttgt ggggtttcta taaaaattac tgcttcaatg
     3481 gtagaccaac cagcagcttt atttggcaat ggatgttttg aaccaggaat ggttaaaaat
     3541 acttatggta caggatgttt cctatatatg aatataggtg aggaactaaa tatagcaggt
     3601 aatggtattt tgacaactgt tgcttggaaa ataggagata aagttacata tgcttatgat
     3661 ggtggagtgt atatagcagg tgctgcaata caatggctta gagatggcat tggagttata
     3721 aaaaattatt cagaaactga tgacatggca aattccatat caagtactgg aggtgtatat
     3781 tttgtaccag catttgcagg tatagcagca ccttattggg accaatatgc tagaggtaca
     3841 atggttggaa taacaggaag tactaaaaaa gagcatatag taagagcgac tcttgagtct
     3901 gttgcacttc aagtcaaaga tgttgttgat tctatgaact tagcctcagg gaaaaaaata
     3961 aaaatgttga gagtagatgg tggaattact aaaaataact ttacaatgca atttcaatcc
     4021 gacatattag gtataccaat tgaaattgca gaaaatcctg agacgacagt acttggagta
     4081 ggatatatgg caggtctttc ttgtggtgtc tggaacagta ttgatgaaat tagaagtgaa
     4141 tttacaataa gcaaaaaata tattccaaat atgactactc aaaaaagaga agaaatatta
     4201 cttggatgga accaagcagt aaaaagagct atgaattggg aaaacgatac caaaaaggtg
     4261 tgtgctaatt aagttacaat agagtatttt acatgtatag gaggaattaa aaatgccctt
     4321 tgataaaatt atattataca taatggctgt gggagtgctt ttaggagcgt tagatagaat
     4381 aattggaaat aaatttggac ttggcgagca gtttgaagaa gggtttaatt caatgggtcc
     4441 attggcttta ggaatggttg gtatagtttg tttagctcca gtaatttcag atgtactagg
     4501 tcctataata ataccaatct tcaatctctg tggagctgac ccatctatgt ttgcaactat
     4561 tttagcgaat gatatgggag ggtatccatt ggctatggaa cttgcacaca ataaagaggc
     4621 aggaatgctt gcaggattgg ttgtgtcatc catgcttgga tgtacattag tattttcaat
     4681 tccagtagga ctaggtctta tagaatatga agacagacca ttctttgcaa aagggttact
     4741 gataggcttg ataacaatac cttttggagg tattatagga ggattaatag caggatttaa
     4801 cttatcaatg gtaattataa atatgatacc agtgataata ttggcagtaa tattggcagt
     4861 aggtttaaaa tttaattcta cgcttatgat aaatgggtca ttagtttttg gaaaatttat
     4921 atctatattg ataacgatag gtttgggagc agcagcattt caagaaataa caggtatagt
     4981 acttataaaa ggcatgacac ctataagaga aggtattcaa gtaattggaa gtattgcaat
     5041 cgtgttatta ggtacttttc caatattaca tttattagtt aaagttctta ataaaccact
     5101 tacaatagtg ggtggaaaat taggtatgga tgccactagt gcagcaggat tagtttttac
     5161 tttagccaat agtattccag tatataaaat gatgaaagat atgagtccaa aggggaaagt
     5221 agtaaatact gcttggctgg tttgtgctac agcagctttg ggggaccatt taggatttac
     5281 agcaggagta gttccagaga tgattacacc tgttgtaatt ggaaaattgg ttggaggagt
     5341 attggcaatt gcacttgcta tacttatgac aaaagattta accttggaag aagaacaaag
     5401 tattaaaatg aagaaagatg cagtgtagaa taaacaatac ttaagtcaaa tttaaattat
     5461 tttaaagtag gagtttaaat aactaaaaat aaattaaaaa gacagacaat taaaaaataa
     5521 tcgaataact aaaacttata aattaaataa aatggcaatc aaataactaa aaatgataag
     5581 ctaactgaaa gtagttacat aattaaaata taaagtatag tttagacata aatggagcta
     5641 aataaataat tatttggctc cttaatttta atttattaca tcaaggaact aaaaatcaat
     5701 catataagct aattatcagc tatgactaaa ggtattcctt tttgagaaaa ctctattatt
     5761 atattatggt ctatactagc agaggtaata atagtatcta tatgttctat aggagctact
     5821 gatgcaaatg aatttatacc aaacttagga ggttcgacaa ctactataga ttttctagac
     5881 ctttcaatca ttttttttct aacaagtgct tcttctaagt gatagtcaga gattccattt
     5941 tcaagagtga tacctccagc gcctaaaata gctttatcaa cattaaattg ttcaatagct
     6001 gacagtgtaa gcggaccaga aaaagaacct tcagtttctc taagatagcc cccaagcaca
     6061 tatactttta tattagtatt cataagctcg tttgcaacta aaattgagtt ggttattaca
     6121 gttagattct ttttatcttt caaatttttt gaaacttcaa gtacagtagt tccagtatcc
     6181 ataaaaatag tatcatttgt atttataaac tcactacatt taatagcaat ctgtttttta
     6241 tcgtttgagt ttattttttt tcttttaaca tagttaagtt ctacgctact tgctataact
     6301 ggtatagcac caccataaac tctttttaaa agaccgatac tctccatatg ggctaaatct
     6361 cttcgtgcag tttctattga tatatcaaac atttgaacta tgtcatttat tttaattata
     6421 ttatctgatt tgagaatact taaaatcttc tcatatcttt cctgtggtaa cataaaatat
     6481 cctccttgga ctagtattat tttagttttg gcataactta caattaagtc agagtttagg
     6541 tatatttgtt aatgtatgta tatttatcaa ataatattat aagtgttatg caagataatt
     6601 ttaccatatt taaaacttac atttaagcaa aaaagtaaaa atagatgttt aataattcaa
     6661 aataactcaa attttataaa acaattgact aaaatatgat tttattatat aatttactag
     6721 tatagagata tggagaagta ctcaagtggc tcaagaggat cccctgctaa gggattaggc
     6781 tgggtaaccg gtgcgagggt tcgaatccct ccttctccgc caattattaa aggctatttt
     6841 tattaaagat agtcttttta taatgtcata aatgattaga tatgtataac taaagaaagg
     6901 agggaataga tgacaacttt aatttttttc atagtgggga tagcatcttt ggtagttgct
     6961 tttgttattg atgggggaca tgttggagca ttatttgtat tttcatcagc acttgttgtg
     7021 tttggtggaa ctataggagc tgttggagct tcgactccat tccctgtgtt taaaaagtct
     7081 ataaagataa tgttattaac ttttaaaaat aaaaagccta ataacataga aaatataatc
     7141 tatttcaaag aaattgcaac aaaggctagg aaagaggggc ttcttagtat tgacaaagaa
     7201 ataagtgaga atgatgaatt agaccctttt attagaaaag ggcttgaact tatggtagat
     7261 ggagtggatg cagttactat aagaaatata ttggaatcac agatacagat aacatcaaaa
     7321 cgacataaag aaggaatagc aatttttgat tcagcaggtg gatatgcacc tacactgggg
     7381 ataataggta cagttatggg attagtacat attttaggta atctttcaag taatccagat
     7441 gcattaggac ctcaaatttc tgttgcattt ttggctactt tgtatggtgt tggttctgcc
     7501 aatttaatat ggttaccaat agcatcaaaa cttaaggcta taaatcaaca agaagtaagt
     7561 gaaagacttt tggtgataga agcaatatta tccatacttg agggtgataa ttctaattta
     7621 atgacagaaa aattaaaaat atttttaaat tcaaaagaac taattgaact tgaagaaatt
     7681 ggtggtagag ctgaataata taaaaggatg gaatgtttat gttgtatgat gaagatgaaa
     7741 aagaagaaaa caatgagcga tggcttttaa cttattcaga cctcataaca ttacttatga
     7801 ttttctttgt aataatgtat tctatgagta atgttgatgc ggagaaatat aaacaattat
     7861 ctcaatctct aaattctgct tttggtggtt cttctggagt tattgaaggt ggtaatagca
     7921 agatagaacc agtagttgaa ccaggaagta atgatttaga tactttacaa aatgctaagt
     7981 ttaaaaaaat tggtgaagaa attcagaagt atttaaacga aaatggtatg gctaattcag
     8041 tatcattaag agttcaagat agaggattag taataagcct taaagataca atactatttg
     8101 atacagggaa agctattgtg aaggataatt ctagagataa gattattcaa ataggtaaga
     8161 tgttaaatga aatgaatagt tacatgagag tagaaggcca tacagacaat atgtctataa
     8221 aaaattcaga gtttaagtca aattgggatt tatcagttat gagagcaacc aatgttgttc
     8281 aacttcttat tgacaatgca ggtatagcac cagataaatt atctgctgta ggatatggtg
     8341 agtttagacc aatagcagaa aattcatctg aagaaggtag gtcaaaaaat agaagagttg
     8401 atatagtttt agttgatagt aaatatgata atgtagaaaa tgtttcaaag gataaatagt
     8461 taaattagct gtctcaatac agaattaatt ctaaaggaga tggctttttt atgcgaaaat
     8521 caaaaataga tacatagtaa gttacctttt tgtaggaata tatggtatct taaatatata
     8581 atatttaaat aatgtataag ctcaattaaa aaatttatag aggatggaaa ataaatggga
     8641 aataaaaata aaaatactaa atataaagat aaaacaagag aaaggtataa taaaaaagtt
     8701 gagaatttaa ataaagaaaa attaaataaa attaataagc aatctaagaa agtagataag
     8761 attgatgata ttattaataa atctagtatt gtattatgtg tacttctagt agtgttttca
     8821 ttgctttact tatttaaact ggttcaactt aatataataa tgatactttt agtaattaca
     8881 gtagctattc aacaaggatt ggtgtcattt ggtgcattta gaaataaaaa ttatgtaaaa
     8941 gctttatttt ctatatttgt aatagtatgt ttcttaattg cttttaaata tgttgtaaat
     9001 cctgtttaaa tatatcaata aacgttctat aattaatata aatagtgaag ttacagtttt
     9061 aatgctatta taaattttat aatttgaagg agtagatttt atgaataggt gtaagtttgc
     9121 aaaattaatt atcacatcaa tgattgtttt attacttaca ggatgtagtt taaagaagga
     9181 agataattat aagttagacc cagaaaatcc gacatcaata caaatttggc attattataa
     9241 tggacagcag aaaatggctt ttgataagct tgttaaggaa tttaatgaca ctgtaggtgc
     9301 tgaaaaagga attattgttg aagcattcag tcaaggtaat gtagttgatt tatcagagaa
     9361 agttttagat tctgcaaata aaaaagttgg ttctgaagat gtaccaaata tatttgcagc
     9421 atactcagat actgcatatg aaatggataa attagggtta ctagtagata tggataagta
     9481 cataagtaaa gaagaattaa atgaatacat agacgaatat gtagaagaag gcagaataaa
     9541 ttctaaggat gagtttaaac ttttcccaat tgcaaaatca actgaaattt tgatgttaaa
     9601 taaaactgat tgggataaat ttgcaaaagc tacaaattca aatataaatg atataaaaac
     9661 aattgaagga ttagtagaga catcaaaaaa atattatgaa tggacagata gtttaacgaa
     9721 agagcctaat gatggtaaag cattttttgg aagagatgct ttagcaaatt atattattat
     9781 aggaagtaaa caactaggag tagaaatttt ttctgttaaa gatggtaaag ttacactaaa
     9841 tattgatgaa aaagttatga aaaaactatg ggataatttc tatgtaccat atataaatgg
     9901 atattttgct tcttatggta agtttcgttc tgatgatgca aaaacaggtg atatattagc
     9961 tcttgtaggt tctagttcag gagcagcata ctttccagat agagtaatgc taaatgataa
    10021 tgagagttat aatattgaaa catctgtttt tcaagcacca cgttttaaag gagaaggaaa
    10081 atatgctgta caacaaggtg caggaatggc tgtaacaaaa tctactaaag aagaagaata
    10141 tgcatcagta gaatttttaa aatggtttac agataaagaa cctaatataa aattctctat
    10201 tgcttctgga tacttaccag taaagaaaaa aacgaatgat ttggatgtta taaaagaagt
    10261 tatgaagaaa gataatttga gtttatctga taaagttgaa ggttcaatag cctcatctat
    10321 tgaacagatt aacagttgta atttatatac taataaagca tttgatggtg gaactaaagc
    10381 tagacaaata cttgaatatt caatgcttga taaagctaaa gaagattcaa ataaaataaa
    10441 tgaattagta aaaagtggaa cttctaagaa ggatgcagta gcacaattta ataatgataa
    10501 aaattttaaa acttggtttg aaagttttaa ggctgagctt gaaaatgcta taaaaaaata
    10561 gatgaattgg cagttagtgt gttgacgagc acaaaggtat aggtggaatg ggtatggaaa
    10621 acaatctaag aaaaaacaat tcaataatga aaaagatgat atttccccta attattgtta
    10681 tgctaattca aacaagttta ttttgtgcca caattttatg gggaggaact attaagaaat
    10741 taaatgataa ttcgtttgac atcttaaatg aaagagtaat taatcgtaaa aattatatac
    10801 aaaatgaaat gttacaaaga tggtcaaata caagtgaaac ggaagctaca attaattcct
    10861 ctgtaaaaaa agtattaaat gaaaaaaatg ccttaattga ggatgtaggc aacaatgaag
    10921 aaattaacaa agatattata aaaagcatat caaatgattt aatatatctt ttgagaaaaa
    10981 attctgttac aggagctttt ataatcctaa gtgacaaaaa tattaacgat aagtcaatag
    11041 atagtaaagg tgaaataagt aggacaggat tgtatataag ggatttagac cctaagttta
    11101 atccaaatga taattctgat ttgttaattg agagaggttc atcagatata gcaagaagtt
    11161 ttggaatatc tatggacagt tattgggaac ctaagttttc atttaaaagt gaaaatgagc
    11221 aagatggaga tgattttttt tataaaccat ttagagctgc aattgataat ccaaatgtat
    11281 caagttctaa tttaggatac tggggaagta agtttttatt gacaaatgca ggtgataggc
    11341 ttatgacata tacagttccc cttatttatg aagatggaac tgtatatgga gtacttggaa
    11401 tagatttaag tgtaaattat ttaagagaat tacttccata ctcagagata aatggaaata
    11461 aaaagggtaa ttatatttta ggtgtagata gaaataatga tatggccttt gaaaatgtaa
    11521 tttcaaatgg atactcatta aaagaaaaat tttgggatgg tttacattct aatttagagc
    11581 agaaggaatc gtataaaaat atatataaga ttaaaaataa agataatgaa tgtggtaatg
    11641 gcaacattta tgctagtgta caatacttgg atttatataa ctccaatact ccatttgaaa
    11701 atgaacgttg ggctcttttg ggtattattg aagaaaaaag tcttttgtca ttttcaaaaa
    11761 gggtagagaa attagttata atatctatag caatttcttt attaataggt ctaataggta
    11821 tttacattgc atcaagatta tttacaaaac caattgttct tttagccaac aaagttagaa
    11881 acagtaatcc atcaaaacca gtaaatcttg ataagataaa tatatctgaa atagatgaat
    11941 tatcttcagc tatagagttg ctcagtgcaa atgttgcaga ttcttcgtca aaattatcac
    12001 agattattga aatggtagat atgccaattg gggcttttga gttatacaaa aacgcagaga
    12061 atgttttttg tacaaaagaa ttttttagcg tgtttactac tgaaatcaaa tttgaagaat
    12121 atggatatat ggaaaaaaat aagttcctga aaaaattaag gggactagat aaatatttgc
    12181 aagaaagtta tgaaacagaa aatacatata tatataagtt tgaatctaaa gataaaaaac
    12241 ttaagtggat aagaatgaat atctttgaag atgagctaaa ggttcttggt gttgttgttg
    12301 atgtcacaaa agaatttatt gagaaaagga agatagaata tgaaagagat tttgatattt
    12361 taacgaatct tttaaatagg agagcttttc attcacagat acaagaaaaa tttaagaata
    12421 aagatgaact taagatagca gcatttatta tgtgggattt agataatcta aaatatatta
    12481 atgatacata tggacatgat gctggtgatg aatatattag gtgtgctgca gacatactta
    12541 agaagtttat atcatggaat ggtatagtat ctagactttc aggtgatgag ttttatgtat
    12601 ttatatatgg atatgaaagc aaagatagta taagagatat tattaattct gttaagaaac
    12661 agatgaataa taccttatta aaacttcatg atggaacaga atttaaaata agggcatcag
    12721 caggaatttc ctggtaccct gatgactcaa ccaactatga tgaattaatg agatacgcag
    12781 attttgctat gtatcaaatt aaaaatacaa tcaagggtga agttagtgaa tttgatatta
    12841 atatatataa taaggattca ttcttattga gtaatagaga agaattaaac aagttaattg
    12901 atggtcaatt agttgagtat gcatttcagc ctattgtaga ttgtaaaggt aaagtatttg
    12961 catatgaagc acttatgaga cctaaaataa aaacttttag ttcaccaata gatgtcatta
    13021 gattagctcg ttcacagtct aaattatatc aaattgagcg tattacatgg tttaaagcac
    13081 ttaaatcatt taaaaaatac aaagaaaaat ttggcgattg caaattgttc ttaaattcta
    13141 ttcctaataa taatttgtca aaagtagatt ttgaggaaat tgaagattta tatggtgaat
    13201 atttaaatag agtagtacta gaaataacag agaatgataa gattaatgaa gattttattg
    13261 aaaaaaagaa agcatatata gaccattgga atatagaaat tgctttagat gattttggga
    13321 caggttacaa cggagatgct gtactattat atataatgcc agactatgta aagattgata
    13381 tgtctattgt aagaggtatt gataaagatg aaaacagaca gaaaatatta aaaaatctta
    13441 tctcatattc taacgaaaga aatattaaag tgatagcaga aggtgtagaa acaaaaggtg
    13501 aaatggaaac actaattaat cttggagtag attatatgca aggctactat attagtaaac
    13561 catcatttat acctgaagat ataagcgaca aaattaaaaa agaactcaag atatgtcatg
    13621 aaaataggaa tgatgtggtt taatagagga aaaattaaat ttatttttat gaacttttag
    13681 tttataatta aaaagaaaat aatataatta taaatcaagg tggtggagct aataatcaca
    13741 acaaatatag atataagaaa gttaaatgaa aatcaattag aagcagttga gcatatagat
    13801 gggccttgca tgatacttgc agggcctggt tcaggtaaga ccagagtaat tacatataga
    13861 atagccaata tggttgtaaa caaaaacata gcaccaacaa gaatcttggc aataagtttt
    13921 accaaggcat cttcaataga aatgaaaaat agagctttga atttaagtga tgacataaga
    13981 cttaataagg taacctatgg tacttttcac tctgtatttt ttaaaattct aagatacttt
    14041 gaaagataca acttagacag tatatttgat gaaaagtcaa agcgaatgac aatcaaggca
    14101 atcctaaaaa gtttaaatat agagaatgct gatgatgatg agaatatagg acaagttata
    14161 aatgaaattt cctatgtaaa aaatgaactt atggataaaa atgaatttaa ttcagaagta
    14221 ttaacaaaag atgagttttt aaaagtatac aacttatacg aagaacaaaa atctaaagtt
    14281 aataaaatag attttgatga tatgcttata agaacttatt atttactttt gaacaataag
    14341 tctgcactag aaatggttag aaatgtgtat aaatatatat tgatagatga atttcaagat
    14401 ataaataaag tacagtttga agttttaaag ttaatttgta gcccattaaa caatatattt
    14461 gcagttggag atgaagacca aagtatatat ggatttagag gtgctagacc agattttttg
    14521 cttgaatttg aacaatactt taataatact aaaaaaataa tattagatat aaattacagg
    14581 tctaaaagtg agatagtgca tactgcaaat agacttatcg acaaaaataa aaatagatat
    14641 gaaaaaatca ttaagtgtag tcagggggat ggaggaagtg ttacctatat ttctccacat
    14701 gattcagagg aagaagcctt atatattgcc agggaaataa ttgacgaaat acaaaaagat
    14761 tatgtagagt attcagattt tgcagtaatt tatagaacta atatacagtc aagagcatta
    14821 gtagatgtat ttatggatat gaggattcca tttgtagtaa aagactcagt aattacaata
    14881 tatgaccatt gggcaagtca agatatacta gcctatttaa gaataggtat taatccaaaa
    14941 tcaaataaag attggttgag aattataaac aagcctttta gatatatatc caaagacagt
    15001 ataaatatgg ttaaggatga aaaagacttt ataacagcat taataaataa gtgtaatctt
    15061 catcctaaac aagttaaaac aataaatgat ttggaaatag atttaagtta tttaaatact
    15121 ttaaatccta aaaatgcaat atcttatatt agaactagtc ttgattatga tagatatgtt
    15181 ttagattatt gtagcaacag aaagataaag actaatggac ttgtagagat attaaatgaa
    15241 cttgagagtt cagcaactaa ttttaatact gttacagagt ttttagagca tattgataga
    15301 gtaaagactg agttaacgga aaataacaag aataagcaga ctgagggtgt catatttaca
    15361 actatgcata gtgcaaaagg attagaattt agaaatgttt atattatagg tgtaaatgaa
    15421 ggaacaatac ctcatgaaaa atcatatgat atttgtaagg aagagaaaaa agaagaacaa
    15481 ttagaagaag aaagaagact tatgtatgtt gctattacaa gagctgaaga aaagttatgt
    15541 ataagctcta ctttaaataa gtatggtaag aaagttgata aatcattatt tattaatgat
    15601 ataaaatctc caacgaaaaa agaaattgac agtattggtg taggggataa ggtataccat
    15661 aaaaaatttc atgaaggaga aataattaaa aaagatggaa ttatgtttac aataaagttt
    15721 aaagatagag agcgaatctt ggatttgaaa acttgtttat tgaagaatat aatttatact
    15781 atttaattta gaatctaaat ttagatagaa atataatgta ttatttatta tgtgcaagac
    15841 aaatagatat gtgtaaaatg tattatttaa cttattagaa aaagaaataa gggtttaata
    15901 tattatttat agttgtttag aaaaagcaac aatgtggaaa atattattta gtattattaa
    15961 agaaaagcaa caatgtgaaa gatattattt agtattattt aagaaatagc taaaaatatg
    16021 aaatatacta tttataatta cacaaataat atcaataaat ataaacagta gtatttagat
    16081 aatttacaag taaaaattat taaaaaaata aaaaacttat tgacaaaaat attataagga
    16141 agtacaattg tattaacaat ttaaggaaac aaatccaatg atagagaaag tagcatatat
    16201 ggatttacag cgagttaggg gttggtgtaa gcctaacaat gaagtctatg tgaagagagc
    16261 tctggaggag attatcagaa actttagtat gataattggt ttacccgcct taagggattg
    16321 agaggataag tacttactta tcaaaaagag tggtaacgcg gatataattc gtctcttagc
    16381 tgtaaagcta agggactttt ttgattttaa aataaaaata tccatacagc ttaaaagcta
    16441 acagtcacaa attttgaaag taatataagt atttatcaat aagagtggta acgcggtaaa
    16501 gtcgtctctt agtcattaaa gactgagaga ctttttttgt aactcgaaac atttaagtat
    16561 taaaatctga gggaaattat aattaaaaga gaggggtagt taaaatgaaa gtatttaaaa
    16621 aattattaag tttaggatta gtattaggat tgacattatc gctagtagga tgtagtggtg
    16681 gaggagaaaa aacaaagtta gaacaaataa aagacaatgg aaaattagtt gttggtacta
    16741 gtgcagaatt tcctccattt gaatttcata aggtagtaga tggaaaagat tcaataaaag
    16801 gattcgatat aatgttggct gaagaatttg caaaagagct tggtgtaaag gttgaaatta
    16861 aggatatgtc atttgatgga ttaataggtg ctttaaatgc agaccaagtt gatattgttc
    16921 ttgcaggtat gtctccaaca ccagagagag aaaagagtgt tgatttttca gaattatatt
    16981 atttaagtag aaatgcagtt atagttaaag atgcagatat agacaaagta aaaactgaag
    17041 atgatttaaa gaaacttaga gttggagttc aagcaggaag tatccaagaa gaatatgtag
    17101 ttaacacttt aaaaatgaca actacaaaat ctttgaaagc aatacctgat ttaattacag
    17161 aattaaaaaa tggaaacata gatgcagttg ttactaatga agcagtttct ttaataaatg
    17221 ttaaaaaata tgatggaata aaaatggcaa atactgaagt tgggaaagat gtaacagaag
    17281 gtatggcagc agcaatcaaa aaatctgaca ataacaaaga ttttatagaa ttattaaaca
    17341 aaaagataaa agaattacaa gatggcaaga agatagaaga atttttaaat gaagcatcta
    17401 ctgaagctgc ttcaaattag taagaaagtt atgtgggaga tgaaattatg agtttagatt
    17461 ttagtttttt aagtagattt gggacttctt ttttggaagg aacaggtgtg acagtatcaa
    17521 tttctcttgt ggcattatgc tttggattta taataggtat aattatctgt atggcaaaaa
    17581 tatcaaagag caaagtatta agagcaatat catcaatcta tattgaggtt ttaagaggga
    17641 caccattatt agtacagata tatatagtat ggtttggttt acctcaatta ggaataagat
    17701 tccctatgtt gtttggtata ccaagtgaat ttatagctag tgcatttgca ttatcagtaa
    17761 actctggtgc atacgtggca gagatactta gatcaggtat acaatcagtt gataatggac
    17821 aaatggaagc aagtagatca ttaggattaa attattggtc aactatgaga tatataataa
    17881 tacctcaagc tataaaaaat atattacctt cattagcaaa tgagtttatt acacttgtaa
    17941 aagaatcttc aataatatct gtaataggag ttgttgagat aatgcgtact gctgatatag
    18001 taaaaaatgc agcatttaga gcattagagc cattaatagt agcagcagca atttactttg
    18061 ttataacatt tactctatca agattagttg gattattaga aaagaaacta tctgtgtcta
    18121 actagatgag agcatgttta gcatttaggg aaaacataga atagtaaaaa ggaaagtgat
    18181 aatatgatta caataaaaaa tttaagtaaa tcttttggag atttaaatgt attaaaaaat
    18241 atagacttag aaatcgctaa aggcgaaata atggttatag taggtccaag tggttctggt
    18301 aaaagtacac ttcttagatg tatgaattta ctagaaatac ctacaggtgg agaaataata
    18361 tttgaaggaa aaaatttggt cgataaaaaa actaatattg atgaggttag acaaaatata
    18421 ggtatggtgt ttcagaactt taatttattc ccacataaga ctattttaga taatataaca
    18481 ttagcaccaa ttaaattaaa aaagatgact aaggaagaag cagaaaaaaa agcagaaatt
    18541 ttgttgagta gagttggtct tttagataaa aaagattcat atccatcaca attatctgga
    18601 ggacaaaagc aaagaatagc aatagctaga gcacttgcta tggaaccaga tatgatgttg
    18661 tttgatgagc caacatctgc tcttgaccca gaaatggtaa acgaagtttt ggatgttata
    18721 aaagaacttg ctaaagaagg tatgacaatg gcaattgtaa ctcatgagat gggatttgct
    18781 aaggaagttg cagatagagt tatttttata gatggaggaa gtatacttga agataatact
    18841 ccagaagaag ttttcggaaa tccaaaacat gaaagaacaa aagcgttttt agcaaaagtg
    18901 ctataatata taaaaaggat gttattgata ttagaaattc aatacatcct tttttagtga
    18961 tggttaatat taattttaaa attaaagtta tattaatatt aactttattt ttacgatttt
    19021 ttgcagaaaa aaaaagagct cggggtaagc tctttttttc caccgaatta atttccgtta
    19081 ctaatccgat ttttaataaa tataaaatat atccttatga aagggtattg ggaatagata
    19141 tattaactaa ctagggttac cagggggata accttaattt attataacaa aatgcgacac
    19201 aaaaatcaat attttttttg aaataaatta taaaagttat ttttataaca agtaagctta
    19261 taaatttaac ttttatagta aaaataagta tatgtataa
//
LOCUS       DAMKMI010000070        19281 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMKMI010000070 DAMKMI010000000
VERSION     DAMKMI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19281
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.071"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            126..464
                     /locus_tag="PC429_002236"
     CDS             126..464
                     /locus_tag="PC429_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF3847737.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(591..1880)
                     /locus_tag="PC429_002237"
     CDS             complement(591..1880)
                     /locus_tag="PC429_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF3847738.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            1960..2664
                     /locus_tag="PC429_002238"
     CDS             1960..2664
                     /locus_tag="PC429_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF3847739.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            2756..3802
                     /gene="selD"
                     /locus_tag="PC429_002239"
     CDS             2756..3802
                     /gene="selD"
                     /locus_tag="PC429_002239"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2804..2806,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF3847740.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            3820..5232
                     /locus_tag="PC429_002240"
     CDS             3820..5232
                     /locus_tag="PC429_002240"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF3847741.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            5346..7229
                     /gene="selB"
                     /locus_tag="PC429_002241"
     CDS             5346..7229
                     /gene="selB"
                     /locus_tag="PC429_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF3847742.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            7334..10072
                     /locus_tag="PC429_002242"
     CDS             7334..10072
                     /locus_tag="PC429_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF3847743.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(10191..11135)
                     /gene="manA"
                     /locus_tag="PC429_002243"
     CDS             complement(10191..11135)
                     /gene="manA"
                     /locus_tag="PC429_002243"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF3847744.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            11308..12669
                     /locus_tag="PC429_002244"
     CDS             11308..12669
                     /locus_tag="PC429_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3847745.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            12757..14646
                     /locus_tag="PC429_002245"
     CDS             12757..14646
                     /locus_tag="PC429_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3847746.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            14661..15116
                     /locus_tag="PC429_002246"
     CDS             14661..15116
                     /locus_tag="PC429_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3847747.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            15175..15492
                     /locus_tag="PC429_002247"
     CDS             15175..15492
                     /locus_tag="PC429_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF3847748.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            15517..16779
                     /locus_tag="PC429_002248"
     CDS             15517..16779
                     /locus_tag="PC429_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF3847749.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            16884..17948
                     /locus_tag="PC429_002249"
     CDS             16884..17948
                     /locus_tag="PC429_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3847750.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            18002..19180
                     /locus_tag="PC429_002250"
     CDS             18002..19180
                     /locus_tag="PC429_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF3847751.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         7770 a         1998 c         3356 g         6157 t
ORIGIN      
        1 aataaaaaat tgttattaaa atgataatta atatcaataa gtattgacaa aattatttct
       61 aaatactatt atatataata taaataataa tgaaaatcaa tatcaaataa atgggaggaa
      121 atctaatgaa cttattagtg attggtggtc atgaaagaat ggaaaaagat tatatggtta
      181 tggccaagaa aaaaggttat aagacaaagg tatatactac catgtcatct aaattaaata
      241 attctatagg aagaccagat gcagttgtca tacttacatc aactgtttca cataagatgt
      301 ctagaacggt tgagtctcaa gctaaaaaac aagatatatt aatcgtaagg cataaaaata
      361 gtagtaaggt agcatttaat gaatgtctag atatggttga tgaatgtctg ggaaattgtt
      421 caaaatgtaa gcttaaagac aaacaaaaac aaaaaaatat ataggtataa aaaatacata
      481 aacataaaat taaaagtact gagataatag ttataattat tatctcagta cttttagatt
      541 tataaggata agtatattat aaatttttta ttataatttc caactacttg ttactttttc
      601 atattttttt tacttctagc tttttttctt tttttcttct ttatatttga agctattata
      661 aataaaacta aaagcactag aatacctgca ataatcttaa caaaagtcat taaaatatta
      721 tttccagtaa aaaatggaag actgctatca agattatttt ttgcaattaa gtctatactg
      781 ttttctagtt taccattatt atatacattt aaagtaccaa ccttagcccc tttttttata
      841 ggcaaatcaa ttttatttaa ctctgttttg gtatcataat tgccttgaga ttcatctttt
      901 tctaagaata tcctataact attttttggt tcataaatca attcatgttg ttttgtaaac
      961 aatacttttt tagtatctgt gtatttttct ttatcaacaa tagtagcaca cttaaagtta
     1021 tcaaatccat aatcaatcag aatacgtgag tctaaatata aatcagcatt agttgaatta
     1081 aatacagcaa cgataactct tctgccatct tttacagcac tcgataaaag acatttacca
     1141 gcagcatcag tatatccagt ttttatacca tcaactatat catattttat atttataggt
     1201 tgacctttgt atgtaatctt atcatgtgat gtcaaaaact tattagtgtt tacaaagtat
     1261 ctctcataag gatatgcttt tgtagcttca aatttaagtg acttagtttt tacaatttgt
     1321 ctaaaagtat cattattcat agcttctcta gctatgagag ccatatcatg agctgttgtg
     1381 tagtgatttg ggtctggtaa tccatgagga ttattaaaat gagtattagt agctccaatt
     1441 ttttttgcct cagagttcat aaggtcaata aatttttgta cattgccacc accaacatat
     1501 ctagctaata caaaagcagc atcattagca gaatgaacca aaagagcatc tagtaactgc
     1561 tttacagtaa aagattcccc attttttaga tacatactag agccatctat ttgaggtaaa
     1621 tctttaattt ctattacttg atttaaatct ttaacctcct ttagaactaa acaagctgtc
     1681 catactttag ttgttgaagc tggataaagc ttagaatttc catccttatt atatagtatt
     1741 ttgccagact catagtccat taaaactgca tgtttagcaa ctatagctgg ttcattatct
     1801 gcaaaagaga tattagagct tcctaaaaat gaagtaaata tcaataaaca gattaataaa
     1861 agtgataaat ttcttttcat tgtgagcctc caaattttaa attcatcttt tagtatagca
     1921 tatatttgtc aataattaaa agaaaggagt gattaatata tgaaaaaaaa agtaaaattt
     1981 atttcgtttt ttgttttatt tattttaata atatcatcaa taatcgttta taaatcgctt
     2041 attttcaaag atgatattta tgttgtaagt aaaaaaaatt tacctaatga aaaaacacat
     2101 gttgtagaga atgaaattaa aggtgaagat aataatcaca ataaagtaac taaagacata
     2161 gataaaaatt ttcaaaataa taaaataact atatacataa gtggagcagt aaatagacct
     2221 ggaattgtaa ctatagagtc agacaagaga ctttatgatg ctgtagaatt actaggagga
     2281 acgacaaaag aagcagattt aaatggagtc aatctatcag taagattaga agatgaacaa
     2341 cattacataa tacccaaaat aggtgaagca acatctgtta caagtaatga tgattccaat
     2401 aaaccaaatc agaggaatga gtcaagaggt gagcctaaaa ataaatccaa taaaaatata
     2461 tctaacgaat caaaagtaaa tataaatgta gcgactatag aagagttgga ttctctacct
     2521 ggggttggag aagctacagc caataaaatt ttacaacaca gagaagaaaa tggacagttc
     2581 agttcaattg aggaaataaa aaatgtaaat ggtattggag ataaaaagta tgaaaatata
     2641 aaagatttaa tttgtgtaga ctaaaatata atacaaaact tacatataga tatgttatta
     2701 taaatatgta tgtatatgtg tgtacatgtg gaaatttagt aaaggaggca cttttatgaa
     2761 aaaagaacaa aaaaaactta cagagatgac aacagcaggt ggttgagccg ctaaaatagg
     2821 gccagaggtt ctggcatcag ttttatctca attgcctaaa aatgataata tagaaaattt
     2881 attagtagga ctagatacag cagatgatgc tgcagtttat aaattaaatg atgatatggc
     2941 acttatccaa actttagact tctttacacc aatggtagat gacccatacg tatttggtca
     3001 aattgctgca tcaaattcac tatcagatgt atatgctatg ggaggcaaac ctttggttgc
     3061 tatgaatatt gtttgttttc catcatgcca tgatatggat gtacttgcag aaatattaaa
     3121 aggtgggttt gataaagtga aagaaagtgg agcacttttg gttggagggc atactgtaga
     3181 tgacaaagag cctaaatatg gtctttcagt ttctgggatt gtaagtccaa ataaagtttt
     3241 atcaaatgct actgcaaaac ctggtgataa gttaattata acaaaaccaa ttggagtggg
     3301 tgtattaaat acagccatga aagaaggtat ggtagaacaa catatagcag ataaagtaat
     3361 agaaataatg atacatttaa ataaatatgc agcaatgagt tttgagaaat ttgatgtaaa
     3421 ttcggttaca gatataactg gttttggtct acttggtcat actttagaaa tggccaaagc
     3481 ttctgaagta agtattgaaa ttgaatcaaa acatgtacca attattgaag gtgcaataga
     3541 aatggcacaa atgggaatta tacctgctgg tatgtataaa aatatgcatt atgtatctaa
     3601 agatgtagaa gttgtaggaa atatagaagt tgcagttcaa gatattttat acgacccaca
     3661 aacatcagga gggttgttaa tttcagtaaa agaagaactt gcagaggaac ttgtaaaaga
     3721 tatgaaatta aatggagcaa tagaagctaa aattataggt tctgtagttc caaaagggga
     3781 aaaatatatt acagttttat aaaatattat agagaaagag tgattgtttt gagcaaaaga
     3841 gaattgtttg caatgttgcc ttcagttgat gaagtattaa gtgataatag aattgtagaa
     3901 ataataaatg aatatcctag aagtttagtt ttagaatctg tgagagaagt aatagattta
     3961 aaaagacaat ttatattaag attaaaagaa gatgcatcaa actctgtaac aatagagttt
     4021 ggtgaaataa tagaatctgc aatagagaga gttaagttaa attactcatt atctttaaaa
     4081 aaagttataa atgcaactgg tacagttatt catacaaatt taggaaggtc tttacttagt
     4141 gaagatataa aagatgaatt atggtgtgct gcttcaaggt attcaaactt agaatacgat
     4201 ttggataatg gagaaagagg ttcaagatac tctcatttaa ctagtacaat aaaaagactt
     4261 actggagcag aagatgtact agttgtaaat aataatgcag cggcagtact tcttgtactt
     4321 agcacaatgg ctaaaggagg agaagcaatt gtatctcgtg gagaattagt tgaagtaggt
     4381 ggttcattta ggattccaag tataatggct cttagtggtg cagaattagt tgaagttggt
     4441 tcaactaata aaactcattt gaaagattac aaggaggcca ttacagaaga tacaaatgta
     4501 ttgatgaagg ttcatacaag taattataga ataatgggat tcacagagag tgtttctata
     4561 gaagaattgg ttaatttagg taaaaaatat aaactacctg taatagaaga cttaggaagt
     4621 ggagttttta tagatttatc aaaatatggt ttatcttatg aaccaactgt attggattct
     4681 attcgacaag gtgcagatgt agtaactttt agtggagata agatgcttgg aggtccgcaa
     4741 gcaggtatta tagttgggaa aaaagagtat atagaaaaaa tgaagaaaaa tcaacttact
     4801 agagcactta gagttgataa gcttacaata tgtgctcttg aagcaacatt gagaatgtat
     4861 ttagatgaga caaaagctat agaaaatata cctactttaa aaatgttgac atataaaatt
     4921 gaagaattag aagtaaaagc aaataaacta tttgaaaaaa taactgcttt aaacttaaat
     4981 gcaaacataa atatagaaga tggattttca caggtgggag gagggtctat gcctcttgaa
     5041 actataagta caaaggtaat atcaataact cctgaacata tgaatgtttc atctttagaa
     5101 aaaaaactaa gacttagtga agcacatata atagccagag tgtatgataa taaatatgta
     5161 ctagatgtaa gaactatatt tgatgatgaa tttgatgtaa tagttgagga attgagaaaa
     5221 gcatttaatt aaataaattt gagttaataa atattaaatt tagaagtata agtatgaagt
     5281 tttgattaaa aaatagctac acacacttgt gactttagtc atagcaggtt tagagggggc
     5341 aaaaaatgaa aaatgtagta attggaacag caggtcatat tgaccacgga aaaactactc
     5401 ttataaaagc attgactgga agagagactg atacgttaga tgaagaaaaa aagagaggta
     5461 tttcaataaa cttaggattt acttattttg atttaccaaa caatagaaga gctggaattg
     5521 ttgatgtccc aggacacgaa aaatttataa aaaacatgtt agctggagca tcaggaattg
     5581 atatagtttt acttgttgta gcagcagatg aaggggttat gccacagaca gttgagcatt
     5641 tagatattct aagcttttta aatatcaaaa atggaatagt ggttctaact aagtctgata
     5701 ctgtagatga tgaatttaga gagctagtaa aagaagatat ttgtgaaaaa ataaaaggaa
     5761 cttttcttga agatgctgaa ataattgaag ttgattctat atctaaaaga ggtatagaca
     5821 ctcttattga aaaaatagat aaaatgagtg atgatataga agataaaaat gaaaactctc
     5881 cagcaagact taatatagac agagtatttt caataaaagg gtttggtact gtaataacag
     5941 gcactttaat agaaggaaag ataagtatag aagatgattt ggttatatat ccacaggtat
     6001 taaaaacaaa aataagaagt atacaagttc atggagaaaa taaagataca gcttatgcag
     6061 gtcaaagaac agcaatcaat atatcaaatg taaaagtgga tgatataaaa agaggagatg
     6121 ttttagctgc accaaactca ttggaggaat caatgatgtt agatgtaaaa ttatcattgg
     6181 taaatcattt agataaagga ttaaagcact gggatagatt aagattgtat catggaacta
     6241 gagagatact ttgtagagca gttccactag ataaagaatt aattgaaagt ggagaaagtg
     6301 gctatgttca gttgagactt gaagaaagca tagtatctaa gaaaggtgat acgtttgtag
     6361 tgagacgtta ttctccaatg gaaactatag gtggaggtgt aataattgac ccatctccta
     6421 aaaaacataa gaaatttgat gaaaaagtta tagaagcatt aaaaataaaa gaaaaaggcg
     6481 aactaaaaga tataatagaa gagtacttaa aaaggaattt aaaaaattat ccaaatataa
     6541 aagaaataat gagctatagt ggtgctcatg aagaagatgt aaaaagagcg cttgaaacac
     6601 ttatatcaga agataaagta tttattatag gaaatatgta tatgcatata aatcaataca
     6661 ataaattaaa agaaaataca ataaagcttt taagtgagta ccataagaag tataggctta
     6721 gaaaagggat actaaaagaa gaagtaaggt ctaagataga gagtaatttt aagactaggg
     6781 agatggatat tttattagaa aaattgtcta cagaaaatgc tataaaaata gaaaataata
     6841 tagtatctct attagatttt gaggttattt taaatgataa gcaaaaagaa atagcaaaaa
     6901 aaatagaaaa aagattaaaa tcatgtggag tttcttctat actgacaata gatgaagttt
     6961 ctgaaggaaa tcataactat gctgaagtat tggagtcaat gataggtaat aaagtagaga
     7021 aattagatga tttgtatata atggacaaag atatttatga aaatgcaaag aacattttga
     7081 taaattatat aaaagaaaat aaagaaataa cattaggaga atacagagat ttgattgatt
     7141 ctagcagaaa gaattgcatg attatattgg aaaacttcga tagaaacaaa ataactaaaa
     7201 gagtagaaaa caaaagaatt ttgttttagc agaaaatgtc tgtttttgct ttaaggtata
     7261 aagaaggaat tataatgtcg agtatcgtat aattaattag tataaaatat taattaagag
     7321 gaagtgtaaa gtattgaaga atataatacc tgatgtaaaa ccagatataa aagaaagaga
     7381 agacatgagc cttttattta taataatggg ctcagtactt ttattattta ttataaaaga
     7441 tatagagaaa tatgaaactt tattacatgc catatatacg atatttttag tagctacagg
     7501 agtaattata tttaatacta caaaatttaa agtagattca ttttctacat ttttaggatt
     7561 aatttttata gctactggag tattggagtg tatatattta tttaattcct tggggattaa
     7621 aactaagtct ataatggaga ttaacatcac aatatcagca ataacagatt tatttcctat
     7681 actgggtgta tacttaagtt ttaaatttgt taaggataac aaacaaattt attcaagtgt
     7741 aattttattt atagcaactg ctacattaac aataacatct ttattcataa tatcaggaat
     7801 ttgtgactat ctgggaaatg gtaaatcaca atatgcttta ggagttataa tatctatatt
     7861 cataataatt atttctatca ttagtggaat tgaattaaat tcaagttcaa ataaaggtaa
     7921 atgggaatat gatgaaaaaa agatttttaa cagaataata gtaataataa tcttatcaag
     7981 agttccaaat ctattgcata tagttattga taatagaaat gtagagagga tattatcaca
     8041 aataataata aatgtagcct tatattattt atataattac atagtatcta agaatattaa
     8101 gaaaacagca ctggaattgc atgatacgaa tgaagaatta acaaaaaaaa cggaaagttt
     8161 aaaagaaaag aataaaaaat taatctatga gacagaaaag atagaggaac ttaagggaat
     8221 acttatgaaa agagaatcta ggctacaatc tacattagat gtagcagtaa atagcatagt
     8281 agtatttagc aaagatggag aaattacata tgcaaataag agctttagaa atacttttgg
     8341 agactataaa gaacaagata aattgactga taaaataaaa aacttcaatg atttgataga
     8401 aagcattcat tatgtgtttg ttcatgagaa gaatgcagag aaacttatta gtacaagcga
     8461 taataaagtt tatcaagcta tatttgcacc tttaattatt gcttcacaaa cagaaggagt
     8521 attgtgtgta ctagaagata agactaagaa aaaggagtac gaacgcaaac ttattgaagc
     8581 aaataagaga agtaaagatt ttttagaaag tattggagat ggcatagtag tgcttgaagg
     8641 taataacaaa atatatgtta ataatgcatg tagggaaatt tttaaaaata atttagagag
     8701 tattgatttt tcattagtat gtaaaagtga agaaagtata gaaaaaagat atgtaataga
     8761 tggtgaagta aagtatgtag agatgagttt ttctcagtat actaatgctg gtactaataa
     8821 aactattatt gtaatacgtg atacaacctc tcgaaaaata tctcaaataa aacttgagga
     8881 aagtcaatca tcatattcaa gatttataga tatattacct gatggcatat gcttattaaa
     8941 agaagattta agtataaact atgcaaataa gtcgttttta gatatgctag cttttacaaa
     9001 catagatgat ataaaagatt ctaatattaa actattaatg aatgcgaata ctgaagaaaa
     9061 aatgaaattt acggataata tggctaaagt tttaaatgaa aatagaagta tacttttact
     9121 ggaatatgaa ttgataaata gttatgatga tatagtagaa gtagaattaa gtgcattacc
     9181 ttttgctata tacaatacta gatacataat gcttattata aaagatttag tacataagaa
     9241 atattccgag caagcagaaa aagaactgct tgaaagattt aaaacagata aaatcaaaac
     9301 ggagtttttt gcaaatatgt ctcatgaact aaaaactcca ctaaatgtta tatctagttc
     9361 aaatcaactg gtagattctt tctatagaaa tgaaaagata aaagattaca ataacaatat
     9421 taaatctcat gtggatttag ttagacaaag ttcttatagg cttcaaagat taataaataa
     9481 cattatagat ttaacaaaga tggagtctgg gttctataca ctaaaacttg cgaaatacaa
     9541 tattgtatct gtaatagaag acttatttat gaatatagaa gagtatgcac ttagaaaaga
     9601 cataaaaatt ctttttgata cagaccttga agaaattaat gtttatatag acaaggttga
     9661 aattgagaga attatgttga atctattgtc aaactgtatt aagtttactg ataatggagg
     9721 ctggatatat gtaagtattc attataaaat agataaagta atcataagtg taaaagacac
     9781 tggagttgga ataccacaag ataaattaga gttgattttt gaagaatttt cacaagttga
     9841 taagacttta tccagaaata ctgaaggaag tggaataggg cttgctatag ttaagaatct
     9901 agtaagtctt catggtggag atatagaagt agtaagtgaa gtgaataaag gcacagagtt
     9961 cttaatttca cttccaatga agggatttag tagtgaacat tacacagaag ataagagaat
    10021 atataatatt caagaaaaaa taaaaataga attttctgat atatattatt aaaaatgatt
    10081 atgagcaaaa ataaaataaa aatactattt gtttaaatat ttaagttgta agaaataggc
    10141 tatcataagg taaattatta tgatagccta tttttagcta ggttttatat ctatatgtga
    10201 gaacatataa tctctaaatt accttcaaaa ctaaaatctc caaagtcgtt tggtagtata
    10261 aaatgcatac ccttttttaa attgtattct acagaattgt gtataagttt tccatttcca
    10321 tccaacacac tgtataaaga aaaaggtgta ttgtgtgtaa aattacattt tccaaaaaca
    10381 tctaatttat aaacactaaa aaattcgttt gacacaaatg ttgtgcattt aaagtctgat
    10441 gttgatacga ttttatagtc agtatcgaaa tttatatgag gaacattcgt aacatctatt
    10501 gatttttgaa catgaagttc acgcttatta cctgaattgt cagttctatc ataatcatag
    10561 actctataag tagtatctga gttttgttga gtttctaaaa tcaatgttcc tttgcatata
    10621 gcatgtatag tgccacttgg aacatagaaa aagtctccct tttttatatt aacctttcta
    10681 agtagtttgt cccattcatt attatttacc atatttacaa attctttatg agattttgca
    10741 ttatgaccta ttataatttc tgcatcatca gaacaatcta ttacatacca acactctgtc
    10801 ttacctaact ctccattttc atttttttta gcatattcat catttggatg aacttgaact
    10861 gataaattat catttgcatc caatattttt gtaagtaaag gaaacttatc accaggtgtg
    10921 tttccaaata attctctatt tttattccac aattcagata gttttttacc tttatatttt
    10981 ccattttcta ttaagcaatc tccattttta tgagagctta tagcccaaca ttcaccagtt
    11041 gttggcgaat ctatttcata attaaattta tctttaagtg cagtaccacc ccatattcta
    11101 tccataaaaa taggttttaa aaataatggt tccataatcc tgttcctcct atgatattaa
    11161 atttatccta caaataatta tatataaata gattataaaa tcaatagtaa aaaagttttg
    11221 tcccttgaaa tgacataaag tataaggagt tattaaataa gataaactat gatatataat
    11281 ataaagttct aaattggagt gataattatg gagttgttta aggcagaaag caaaaaatat
    11341 aaaaaagaat ttttaaaatt attggagcaa tgggttagta taccttcatt ttatgataga
    11401 aaaactgtat ctaaagacat gccttttgga aaaggagtat atgatgcatt aaactggttt
    11461 gaaaatctag gtagagaaaa caatttcaaa gtaaaaaata tagataatca tgctgttcaa
    11521 attgaatatg gaaatggtaa agagtatgta gatatatttg gacattgtga tgttgtaaat
    11581 ccaggtgaag gatgggatag tgaaccattt aaactaaata ttataggtga taaattagtt
    11641 gctagaggtg tatctgataa taaagggcct atgatagtga attttttagc actaaaaatg
    11701 ataaaggatt tagatattaa tttaaaaaga aaggttagat taatagctgg gggaaatgaa
    11761 gagagtggat ttaaatgtat aaagcattat tatagcaaag agccatatgg tgtatgtgga
    11821 tttactccag atgctaaatt tcctgtatta aatggagaaa aaggtggagc aataataaag
    11881 ttaatttcaa atatagacga taaatcttta tatataagtg gtggaataga gtttaataca
    11941 ataccagata aagtttatat aaagaatgta gaaaaattgg gtaaagataa tatatgtttt
    12001 gatattaaca atatatcgat aaattatgat aatggcaatt atattgtgta tggaaaaggt
    12061 ggtcattcat ctaaacccga aaaatcaata aatcctatat tagctacaat taaacttttg
    12121 tcagaaaata ttgatgaaaa atggacaaaa gatttatata agttgataaa tcaagataat
    12181 ataaatggaa acttatttgg tttaaatatt gaggggaaat gtggaatttt atcaatggtg
    12241 ccaacaataa taaatatagt agatggaaaa ttagaagtag ttttaagcgt tagatatcca
    12301 gaaatattaa ccattgaaga tataataaaa aaatttaatt tgtatatgga gcaaaataat
    12361 attaataaat ttgagcttat tggggaaaat ttaaagcaag caaattatat agatagaaat
    12421 tcaaaacttg taagaagttt gcatgatata tatataaagt attctggaga tttaaaaaat
    12481 gatgttagag taactagtgc aggaagttat gcttctgaaa tgaataattc agtaatattt
    12541 ggttgtgaat ttcctgatgg tagctttggc aatgtccata gtgcgaatga atttgctagc
    12601 ttagatagat ttataactgc aataggtata tatgcagagg caataattac attatgtaat
    12661 aaaatataaa tacaaaatgt cccttatata gacatattat atatacaatt aaaattttac
    12721 ttgatatata attgaaatca aagagaaggt gatacaatga taactaagaa acatgcaata
    12781 atagtaaaga gtttaagcaa taaagatgga tatatgacat caaacgaatt agctatcaaa
    12841 ttggatgtgt ctactaaaac tattaaaaga tatatagcag atttaaatag tgttttaagc
    12901 aaatatgatt tggtaatagc ttcttctaga ggtataggat acaagttaag cggttctaaa
    12961 aacaatatag ccagagctgt taaagaagca aataaatata ttgatggttt tttagatgac
    13021 tctgaagagt caagaatgtc aaatataata tgtatgctta ttaatagaaa ttatatgagt
    13081 atagaagcaa tggcagaaga attaaatcta agtatagccg ctataaataa attgtctagt
    13141 aagcttaaaa gaaaacttga aaagtatgac ttagttataa aatctaaacc ttactatgga
    13201 agctatatac atggtgaaga aattaatata aggcaattaa ttactgacta tgcaataaaa
    13261 ttagatgaaa aaagtaaagt gaaggtgttt ttagatgaca ttagtgaaaa cgatatacac
    13321 tgtatagaaa gtattttaga aaagcactta agagaaaaag atacaataat ttcagataaa
    13381 gattttaatc ttttgctttc aaaaataatc gtttctgtat ttaggagtaa gagaggtcat
    13441 agtaacaata taaacttaat ggatacaagt tatagatttc ataactacta ctttatagaa
    13501 aatctaatga aagaaatatc tgataaaatt ggatttaaac ttattgaaga tgaagtaatt
    13561 tatatttcaa attattctgg tgtaatagcc tataaaggta cacaaggaag aaaaaacaca
    13621 agtgaaattg aggaacgaat aagtactgtt ataagtagtg cacttcaaga tatattcctt
    13681 atttcaggta gtgattatac aaaagatgat gagtttatgg ttgcaatctc tgaccatata
    13741 aagagatttt taaatagagc tagagctaat gtaaaatcaa ataatccttt acttcatcaa
    13801 ataaaagaga agtttcctat agcttttaat ttagccgtgt ttttatcaaa taaactagaa
    13861 actgagttta atttaaaact tgatgaagat gagcttggat atatagctat ccactttgct
    13921 gcttcaaatg aaagaatgaa aaaaaatact agtaagaaaa tttgtatagt ttgtcattat
    13981 gggataggaa caggacaact tatttctgaa aagctaaaac agaatataag tgatttaagt
    14041 gttgtgggtg tttatccagt tagatatctc gatatggcta taagtcaaga tgttgatttg
    14101 atagtatcta cagttgaatt aaaaggatat gaaaaacctg tcttatatat agaaaatata
    14161 tttgatgata gcttaattga aaacgtaaac aaagcctttt atgaaaagga agaaagaaga
    14221 aagataataa gtaacatgtt tgatgaaaaa gcttttttca gtataaaggc tagtactaaa
    14281 gaagaggtca taatttcact ttcaaataaa ctaaaagaaa gagattttat agaggaaagt
    14341 tctataaaaa gtataataga tagagaaaat atttcatcaa ctgaaatagg aaatctagta
    14401 gcaatacccc acacaatagt gaagggagat aaaaaatcta taataggagt tggaatactt
    14461 gaaaatccaa taatttggga taagcaagag gttcagctag tatttatggt attttttaat
    14521 actaaagaaa aacataattt ctcgatattt aaataccttt ataattttat aaaagatgaa
    14581 ggaggagtaa gaggaattat taaaatatgt gactttaata aattaatggc acttataggt
    14641 aattaaggag gaaattaata atgggaacaa acatatttaa caaagaatat gtatttttaa
    14701 atgttgatgc taagtcaaag gttgaagttt taaaatttat atcaaaaaaa gctaaagact
    14761 taaacttggc agaagatgag agcttagtat atgaaggttt aatggctaga gaagaacaat
    14821 ttactacaaa tttaggagaa tctatagcaa tacctcacac taaaaatgat gcaattgaaa
    14881 atccagcagt agtagtattg aagtttaatg aagatgttgt ttggaatgaa ggtgaggata
    14941 aggttaaatt agcaataagc ttacttatgc caggaaaaag taaagagaat attcatctta
    15001 aattattatc atctctttca agaaaattaa taaataaaga atttaaagat tctcttttaa
    15061 aaagtgataa tgttgaagaa atatctaact taataaatga agctttaggt ttataattaa
    15121 acactaaggt tataaaataa aaaaataatt aactaaaaat aggaggaatt tgaaatgaat
    15181 aaaaaattag tagcattatg tgcttgtcca atgggactag ctcacacttt catggcagca
    15241 gaagcaatag agcaagcagc aaaggcatta ggttatgaag caaaagttga aactcaaggt
    15301 gctgatggtg tacaaaatga gttaacaaga gatgatatat taggagcaac tatgataata
    15361 catgcagtag caataacacc agaaggtatg gaaagatttg atggttgtga agtttatgaa
    15421 gttgaattac aagaagctat aaaaaatgca gaaggtgtta taaaagaaat agaagaagat
    15481 ttaggaatat aataaattta ttgtggagga aatagaatgg caattaagaa aaaagttata
    15541 aacagttcag gtgcttctac aacaggagga aataatgtag gaaaatctac tccatctagt
    15601 ccaactcaat caaagggaaa tggaaaatgg aaagaaataa gtaaacatat aatgacaggt
    15661 atatcatata tgataccagt tttagtaatg ggtggactta taggggcctt atctcaatta
    15721 ataccatatg ctatattagg acttgaccca tctgtaggta tagttgatgc tatgaactca
    15781 ggagaattta cagggttcaa gcttagtctt ttaaatatag cacaattaat gtcaaatttc
    15841 ggatttactt tgtttggatt tgcgatacca ctgtttgcag cattttgtgc aaactctata
    15901 ggtggaaaaa cagctttaat tgcaggattt ataggtggat atatagctaa taaacctgta
    15961 ggtgtaccac aattcgttga tggacaatgg actgaagttg ttccagttgc atcaggattt
    16021 ttaggtgcaa tactaatagc atttataata ggatattttg taaaatggtt aaataaatct
    16081 ataaaagttt ctcataactg gttagctttt aaaacaacat ttttaatacc attaatagcg
    16141 tcattagctt gtatggtatt gatgatattt ataataactc catttggtgg attaattaat
    16201 gaaagtatga aaaacttctt aacagcagca ggagcagctg gagaatatgt atatgcaaca
    16261 gctttggcag cagctacagc attcgactta ggaggaccaa taaataaagc agcaggattc
    16321 gttgcacttg gtttaactac agagaatgta cttccaataa cagcaagaac aatagctata
    16381 gttgttcctc caattggatt aggattaaca acattattag ataaaagatt agtaggaaga
    16441 agagtatatg atagacaatt ctatcaagca ggaaaaactt ctatattctt atcatttatg
    16501 ggtatatctg aaggagcaat accatttgca cttgagagac caggttttgt tataccttta
    16561 aacatagttg gaagtgtaat aggagctatt acaggtataa ttctaggagc aattcaatgg
    16621 ttcccagagt cagctatatg ggcatggcct ctagtagata acttatttgg atatataata
    16681 ggtatagcag ttggtgctat attcatagca gtaggaaata tattctatag aaataaatta
    16741 ataaaagatg gtaaacttgt tgtagattat atcgactagt atttaatttt tactatctat
    16801 aaataacttt ggctggtaat atggttttaa tcaatattac cagccttacc tatatgatta
    16861 attaaaggcg gtggagataa gatatggata agatagaaaa agtaagagag tattttagag
    16921 aatatgatat agatggattt cttataaata gtcctacaaa caagttttat gttggaaatt
    16981 tatttagctc ttcaggttat gtttttataa ctaaagaatc acaatatata atagtagatt
    17041 ttagatattt tgaagaaatt aaaagaaaat ctagtttgtt taatgtagta ttaatggaca
    17101 aaactagaac tcattttgat ataattaatg atatttgtag agagcagaat ataaaggaaa
    17161 ttggatttga gggaaatgaa gtatcttttg atttatatag aagtatgagt aataagttaa
    17221 gtgcaacctt aaaatcagtt gatttatcta cattaaggga aacaaaaaat gaagacgaaa
    17281 taaaatatat aaaaaaagct tgtgaaatag ttgatgctac attttatcat atagtagact
    17341 ttataaaggt tggtatgact gaaaagcaag ttgaaaatga aattgttaga attataaaag
    17401 atttaggtgg tcaaaaagaa tctttcgata caatagttgc atctgggctt agaggagcat
    17461 tacctcatgg aaaagcaagt gaaaaagtta tagagtatgg agattttgtt acctttgact
    17521 ttggtgctaa gtataataat tattgttcag atattaccag aaccatttgt atgggtacta
    17581 taaataaaga gttagaagaa atatataata tagtaagaaa agctaatgaa gagtgtataa
    17641 gggttttaag accgggaatg actactggag agatagataa agttgcaaga gacataattg
    17701 gttcatatgg atatgccaat aattttggtc ataatctggg tcatggtgta ggaattatgg
    17761 ttcatgaata tcctgcatta gctcctgaat caaatgaagt attaaaagaa ggaatgattg
    17821 taactataga gcctggtata tatgttccaa gtcttggagg cgtaagaata gaagatgatg
    17881 tacttataac acaagatgga tgtatgagat tgactacatc tactaaggat ttaatagttg
    17941 taagctaaaa ataaattaga taaatatttt ataacaataa gaaaagaaga ggtaaattta
    18001 aatgtataat tttgatgata aaccagatag agtaagtgaa aagtgtagaa aatgggattt
    18061 aaatattata agagataaat ttggagatat tagagaagat tttataccaa tgtggattgc
    18121 tgatatggat tttaaaatac caacagaaat agaaaacaaa tttattgaag ctgtaagaag
    18181 aggtgtattt ggatatacat actgttatga tgaattttat gattcagtta ttggttggca
    18241 aaaaaatatg catgaggtaa atgtagaaaa agaatggatt acattaactt atggaactgt
    18301 atcaacactt cattatgtag tacaagcttt ttgtaagaaa ggtgatagta taatattaaa
    18361 tacaccagtt tatgacccct ttgaatcatc agcaaaaaaa caaggtgtaa atgtaatttg
    18421 taatacatta gatgttgtaa atgatagata ttatataaat tttgataaat tagaagttca
    18481 aattaaagaa aataagccta aattaatgat gttttgtaca ccacataatc catctggaag
    18541 gatatggaca attgaagaaa tgacaagggt agcaactata tgtaaagaaa ataatgttat
    18601 tttggttgca gatgaagtac atgcagagca tattcattat ggtacattta attcaatact
    18661 taagattgga aaagagcttt tagaaaatgt tattctattg acatcaccaa ataaaggatt
    18721 taatcttggt ggattaaaga cttcttattc aatagttata aataaggata taagggacaa
    18781 atttagaaat aaattaaagc aaaattctat aacttctcca aatgtgtttg gaataatagg
    18841 attgataacc gcttataatg aatgtcatga gtggttaagt ggtgtaaatg aatatataaa
    18901 atcaaattat gaacttttag aaacttgggt aaataaatat aataagataa aattgatgaa
    18961 aatggaatca tcttacttag cttggatgga tataagtgga ttaggtatta gtgcaagtga
    19021 gtttacagat aaattggcta tagatacagg agtattatta gaagatggaa gtcattttgt
    19081 aaaagatggt gagaagtatg ttagaataaa tttaggaact caaaaggaaa atgtaattga
    19141 agccttaaat agaatggatt tatttttaaa gtctttataa aaatttggtt gaattagtat
    19201 tttatataaa tttaaactat taatatggta tatacataag tagaatttgt tttagattac
    19261 ttaagtatat accatatttt a
//
LOCUS       DAMKMI010000071        19152 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMKMI010000071 DAMKMI010000000
VERSION     DAMKMI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.072"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            225..1826
                     /locus_tag="PC429_002251"
     CDS             225..1826
                     /locus_tag="PC429_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF3847752.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            2045..3643
                     /locus_tag="PC429_002252"
     CDS             2045..3643
                     /locus_tag="PC429_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3847753.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            3933..6131
                     /gene="slpA"
                     /locus_tag="PC429_002253"
     CDS             3933..6131
                     /gene="slpA"
                     /locus_tag="PC429_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF3847754.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASSSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            6377..8719
                     /gene="secA"
                     /locus_tag="PC429_002254"
     CDS             6377..8719
                     /gene="secA"
                     /locus_tag="PC429_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF3847755.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            9133..11004
                     /locus_tag="PC429_002255"
     CDS             9133..11004
                     /locus_tag="PC429_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3847756.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            11113..11820
                     /locus_tag="PC429_002256"
     CDS             11113..11820
                     /locus_tag="PC429_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF3847757.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            11853..13985
                     /locus_tag="PC429_002257"
     CDS             11853..13985
                     /locus_tag="PC429_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3847758.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(14038..14433)
                     /locus_tag="PC429_002258"
     CDS             complement(14038..14433)
                     /locus_tag="PC429_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF3847759.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            14660..17071
                     /locus_tag="PC429_002259"
     CDS             14660..17071
                     /locus_tag="PC429_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF3847760.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            17368..18945
                     /locus_tag="PC429_002260"
     CDS             17368..18945
                     /locus_tag="PC429_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF3847761.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         7806 a         2132 c         3594 g         5620 t
ORIGIN      
        1 tttaaataaa gcagatatct tttatatata taaatataaa ttcaattttg aatatgttta
       61 aaaagaagtt aaaaataatt ttttaaattt aataaaataa aacactaata tatagacgag
      121 atatattcat atcatatata ctctataata tatggaaaat aaagagaaaa taaatggaaa
      181 ataatcacaa tataaatata aaatataaaa atcatcaggg ggatatgaaa atgaacaaaa
      241 aaatattatc attaggtcta gcagtatcat taatcttagt aaacttcaaa agtgtaaatg
      301 catcatcagt agtagaaaaa atatacggta aagatagata tgaaacagca gcaaagatag
      361 ctgataaaca gacttatgaa acagtaattt tagtaaatac agaaaaatca cttgcagatg
      421 gattaagtgc aagtggatta tcgggagcta caaaagctcc aatactattt acacaacaaa
      481 ataagatacc agcagacaca aatagatgtc taaaaaatat caaaaaagca tatataatag
      541 gtacagaaga tactataagt aaatcagtag aaaaagaact agattctaaa aatatagaag
      601 tcaaaagaat tggtggagaa gatagactaa aaacaagcta tttaatagct aaagaaatag
      661 caactataaa aaaagtggat aaagtactat taactaatgc atatagtgga gaagcagatg
      721 caatgagtgt atcatcagta gctactagag atggagctcc aatcatactt acagatggaa
      781 agagtgtgcc ttttgatgta aaaaatattc aatcatattg tataggttca gaggagataa
      841 tgagtaatcc tctagttaaa aatacaaact cagtaagaat agaaggaact gaccgatttg
      901 aaactaacaa aaacgtaata gactactttt ttaatagtgc agatggattc tatgtatcag
      961 atggatacca attagtagat gcaatagcag ctgcaccact tactaaaaac tctccaatgg
     1021 tactagtaaa tgatggaagc gataaaactg tattagaagg agctaagaat ataacctctg
     1081 taggtgaaat aaatgagaag gtaatacaac agtgtataaa tgcttcaaat tcaaatgggc
     1141 aacccccaac aattacagtt ggaagtacag aggtatataa gggtgaaaag tttgacactg
     1201 gcaagttaaa tatagtagct aaagataaca cagggaaggt attaccaata gaagttgatg
     1261 ggtttataga tacaaataga gtaggtacat acatattgac attaaaagct acagacgaat
     1321 ggggaaaaag cacaggaaaa agagtagaaa taaaagtgtt ggatgacaaa tctcatgatt
     1381 acaacagccc agaatttaaa aaaatggtat ctactgaaat gtataactta atcaattcct
     1441 atagaaaaga aaaaggcaaa gagcctttgc tagtgtctag taggctagaa ggtatggcaa
     1501 atgcatggtc taagtatatg atggataaaa aagtatttgc acattatata gatggaaaaa
     1561 atgctccaca agtattttct gaatttggaa tgagaagtga ggaaaatata gcatatattt
     1621 atatagattc taagaacgtt caaacgacac aagatgcaaa agacttggca aaggctatat
     1681 ttgaggtgtg gaagaagtct ccagagtaca atgcaaacat gcttagtaat gagttttata
     1741 gtactggatt tgggctttat atattatctg atggtcaagt gcatgctact caggagtttt
     1801 taaatggaaa tgaaggtagc ttgtaaaaaa ttccataaat cttgtttttt tgttaattta
     1861 ctgatattat aaaatgaaaa tcacatctaa agtacttttt gtaatctgtt ttgttattgc
     1921 tttacaggct atggtatttt aggtgttttt tgttttagta gtttatagat tttgagggaa
     1981 tcactactga ggatgtaatt tatgattagc tgaattatat aaaaaaatta gtaggaggag
     2041 tttgatgaag gtaaataaga gagtattatc aataggattg acaatatcat taataatggc
     2101 aggagcacca aatatcaatg cactttcgag catagaaaag atacagggga aagatagata
     2161 tgagacttct gcattgatag cagataaaca aatatatgat acaatcatat tagtaaatac
     2221 tgataattct atagtagatg gattaagtgc aagtggatta tctggagttg caaaagctcc
     2281 tataatgttg gtgcaaagag ataagatacc aacagatgtt gaaaaaagac taaaggatgt
     2341 taagaatgct tatgttatag gtacagagga tacaataggt aaatctgtag aaaatcaact
     2401 taagaataaa gggattgaag ttaaaagaat tggtggggaa gatagaataa agactagcta
     2461 tcttatagct aaggagattt cagctattaa accagtaaat gatggtgata aggtttttct
     2521 tgtaaatgga tacacaggtg aggctgatgc aatgagtgta tcttctgtag ctgctagaga
     2581 tggagttcct gtgatactta ctgatggaaa gagtatacca tttaaagttg atggcgttca
     2641 gtgctattct cttgggtcag aggagataat gagtaatgaa cttgtgagta agactaattc
     2701 agttaggatt gctggtaagg ataggtttga aactaataag aaggttatac aacgctttta
     2761 taaaggaaca aagaagtttt atgtttctca agggtataag ttagttgatg ctgtggctgg
     2821 ttctccgttg gctaaggaca aacctatagt acttgttaat gatggaagtg ataagagtgt
     2881 gcttagaggt gctgatgaag ttacttcttt aggtggaatg gataagaagg ttgttgacca
     2941 atgtattagt tctgcatctg ataaaaatac aatgcctaca attactgcta atgatgtaga
     3001 gatttctgta ggggataagt ttgataatag tatgttgaat atagttgcta ctgattatta
     3061 tggaaatgat ttaaaggcaa atattaaagg aaatgttgat attaacaagg ctggaacata
     3121 tgttttaaat ataagtgtag tggataattt aggtcagaag agtgagatta gtgtaaatgt
     3181 taaggtagtt gtaaatgcga gtacaaaaga ctctaatagt tatgagttta aggctatggt
     3241 atctaatgag atgtatgatt tagttaattc atatagaaaa gaaaaaggca agaagagttt
     3301 aagagagtta gactctttag ctggaatggc aaatgcatgg tctaaatata tggaagataa
     3361 gaaggtattt gcccatgaga ttgatggtaa aaatgcagct gaagtatttt ttggctttgg
     3421 agcaagaagt ggtgaaaata ttgcatatct accaatgaat gttaaatctg tttatacttc
     3481 taaagatgca aaagaaatgg ctgagtctat ttttgattta tggaagaagt cttctaagta
     3541 taatgaaaat atgttaaaag aagaatttta tagctttgga tttggtatgc atgtttctag
     3601 taaaggagaa gtgaatgcga caatggaatt tttaaatagt tagattttat attaatttac
     3661 attggaaaag atatagtaaa tttgtaaaaa taaatatata aaattaaaat aacttataaa
     3721 attataaaaa ataatttaaa aataataaaa atgatgaaaa aacaattaat ttgttggaaa
     3781 aaacttcaat taattttgca taaattattg aatttatatt ttatatagtc tatcataaga
     3841 gtgtgagaaa tatttggatt atatgaacat ggaaatttaa tgtacataat taaagagatg
     3901 taattaatat aatgttggga ggaatttaag aaatgaataa gaaaaattta gcaatggcaa
     3961 tggcagctgt tactgttgta ggttctgctg ctccgatatt tgctgatact actgttaaag
     4021 aagaaggata tacggtagta caagacaaat atgaaaaatt acttaaagaa ttaaaggcaa
     4081 agataaaaga tggaactatt acatcagttg gtgttgaatt tgatggaaaa ccaattacta
     4141 cattagcacc aaaggcagat ggtagtgata aagatgcaat agcagaacag ttagaaactc
     4201 ttactaaaaa ccaacttaaa ggtttaggtg atggaaaata tgtagatttt aaaattactt
     4261 atggtgctaa agcagaagtt ccagctgctt cattaagtgc agatgatata caaaaatatg
     4321 cagatcaaat aaatgcttct gagaaaatat tagttgaagt agcagcagga tctgaagcag
     4381 gtatagcaaa atttgattct gttaacaata aagttatagc aggagatgct cctcttaaag
     4441 ttaaagatgc agtgaaagcg actgttacta ctaatggtag taataaaaaa gttcttacaa
     4501 tatcagctgc agctggatta tcaggatttt catatggaac acttaaagac acaggtgcaa
     4561 gttcatctga tgttgatgca ataactcttg acactacaaa tgcaacaata acagaaggtg
     4621 atactaaagt attagatttt gataattcat ttaagtttaa cgaaagtact aaaaaagttg
     4681 gtagtttagt tacaccaaat acaacaaaca ctccagcaga tcctggtact aaaactactg
     4741 ttagagtgat aaaagctgtt gaaaaaacaa tagatgtaag ttcaaattca actacaaaag
     4801 ctaaagattt agctaaacaa tatgtattca cagatgttag tgatactgat ccagaaagtt
     4861 taagttatat gcttaaaaac ataaatgatg gaaaagttgc tgttaaaaat tctgatggag
     4921 attatgaagt aactatattc ccagaaggaa aaagattaaa tactttatct gcttcttcag
     4981 ctaaaactat tttaggagat aaagatactc cagcaaaaat agttttaaag gcttcaacaa
     5041 caaagaaatt agcagactat atagatgatt taataactta taacaatagt tattcaaatg
     5101 tacaaactgt agcaggtagt gatagaatag agactgctat agagctaagc cgtaagtact
     5161 ataactctac tgacaaaaat gctttatatg gagatcctgt aaataacgtt gtattagtag
     5221 gttctcaagc tatagttgat ggattagtag catctccttt agcagcagaa aaagatgctc
     5281 ctttattgtt aagttctaaa gataaattag attcatcaac aagagctgag ataaaaagag
     5341 ttatggactt aaattcttca acaggaataa aaaataataa agaagttttc atagcaggtg
     5401 gagtaaattc tatatctaaa gacgttgaaa atgaactaaa agatatggga cttaaagtaa
     5461 ctagattatc aggtgatgat agatatgaaa cttcattagc gatagctgat gaaatagata
     5521 taaatgataa agcttatgtg gtaggtggaa caggattagc agatgctatg agtattgctc
     5581 cagttgcatc tcaaataaaa gatggtgaag ctactccaat agtagttgta gatggaaaat
     5641 ctgacaagtt atctaaagaa gctgaagatt tcttagatga tgcacaagta gatataatcg
     5701 gtggagaaaa tagtgtttct gctaaaatgg aagattatat agatgatgct actggaaaat
     5761 ctccagaaag aattagtggt gctgatagac aagcaactaa tgctgaagtt ataaaagaat
     5821 actttgataa agatggagtt tcaaattact tcttagcaaa agatggttca actaaagagg
     5881 atcagttagt tgatgcatta gcagcagcag cagtagctgg aaactacggt tctaagcata
     5941 atgaagatgg agatataact acagatgctt ctcctgctcc aattatttta gcaactgata
     6001 atttatcagc tgaacaacat gtagctgtaa gtaaaactgc tactactaat ggtgctaaga
     6061 acttagtaca agtaggtcaa ggaatagctg attcagttgt tagcaaatta aaagatttat
     6121 tagatatgta atataaattt tatatattga ataaaaaagg tttctctttt gagaaacctt
     6181 ttttatttta aacataaatt tttataaata aaaagaggct aattagcctc tttttaatgt
     6241 gagaaacact taaaaaataa gatgtaatta tttatttaag tatcattatt atactatacc
     6301 ttaaaataaa tgtctactat tctataattt taaggtataa tagtaagtgg aaaataaata
     6361 aggaggtaaa ttaaatatgt cagttttaga tgtaatatta gacaaaacag atgaattaga
     6421 aattaaaaaa ttaaataata tagtagataa aatagatgct ttagaagata aaatacaaat
     6481 actagatgat gaagcattaa aaaatatgac gaatgtattt aaggataagt taagtaaagg
     6541 agaaacctta gatgacatat tgccagaagc atttgctgtt gtaagagaag tatcaaaaag
     6601 aatattaggt atgcgtcaat atagagttca attaattggt gggattgtat tacatcaagg
     6661 gaaaatagca gagatgagaa ctggagaagg aaagactcta gtaggagtag ctccagttta
     6721 cttgaatgca ttgactggta agggggtaca tgtcatcacg gttaatgatt acttggctca
     6781 acgtgataaa gaacttatgc aaccagtata tgaattttta ggtttaacag taggagtaat
     6841 tattaatggg caacagccag tagaacgaaa attacaatat caatgtgata ttacttatgg
     6901 tacaaacaat gagtatggat tcgattattt aagagataat atggtaacgc aaaaatcaga
     6961 gaaagttcaa agagaactaa actttgctat agttgatgag gttgattcaa tacttataga
     7021 tgaagctaga acaccactta taattgctgg tggaggagat gaaggtacta agctgtatga
     7081 acttgcaaat gcttttataa agaatataag agatgaagat tttgaaatgg atagaaaaga
     7141 taaaaccata gcacttactg caagtggtct tagtaaagct gagaaatttt ttggaataga
     7201 aaatataact catatagata atatagaaat ttttcaccat gtaaatcaat ctctaagagc
     7261 acataaatta atgaacatag atgttgaata tgttgttaga gataatgaga tactaattgt
     7321 agatgaattt acaggaagaa taatggatgg aagaaggttt actgatgggc ttcatcaagc
     7381 aattgaagct aaagagggag taaaaataaa tgatgaatca aagaccatgg caacagtaac
     7441 atttcaaaat ttctttagat tatactctaa attatcagga atgacaggta cagcaaagac
     7501 agaggaaagt gagtttgaag ctacatataa gattaatgtt gttcagatac caactaataa
     7561 accagttata agagctgatt tacatgataa agtattcaaa acagaacgtg caaagtacaa
     7621 tgctgttgta gaagaaataa taaggataaa taaaactaag caaccagtgc ttgtaggaac
     7681 tgtttcaatt gaaaaatctg agaaattatc taagctactt aaaaagaaag gtgtaaagca
     7741 cgaggtctta aatgctaaac aacatgataa ggaagctgaa ataatatcta aagctggtaa
     7801 attaggttct gttacaattg ctactaatat ggcaggtaga ggaactgata tatcacttgg
     7861 agctggagat aaagaagaag aagaaaaggt gaaagagtta ggtggcttgt atgttatagg
     7921 tactgaaaga catgagacaa gaagaattga taatcagctt agagggcgtt cgggaagaca
     7981 aggtgaccct ggaacttcta gattctttgt aagtttagag gatgaagtaa taaaattgta
     8041 tggtggaaaa agtatagaaa aaatattaaa gagaatgaat gctaatgaag atgacgcgtt
     8101 agaaagtaag gctttgaata gagcaataga aagagcacag aaaggcattg aaggtaaaaa
     8161 ttttgaacaa agaaagaatg ttcttaagta cgatgatact attaatgagc aaagaaaagt
     8221 aatatatgat gaaagaaata aggtattgga tggttctgat attgaagaag agatacaaaa
     8281 aatggttatg gatattatta cggaggctgc tgaaaagtac ttaaaaaaat ttagagacta
     8341 tcatggatat tttaagtatt tatataatac ttttatgcca gctgatactt tattaattcc
     8401 agacttagat aaaaaaaatg ttgaagaaat agtaaatcaa acatatcaga tttcaaagcg
     8461 tgtttatgat cttaagaaga tgatgattgg aattgaagaa ttggctcaat tagagaaacg
     8521 agtattactt caagttgtgg atacttattg ggttgaccat atagatgcaa tggatcaact
     8581 aagacaatat ataggtctta aatcttatgc acaaaaagat ccatttaaag agtatgctat
     8641 agaaggttat gaaatgtttg aagctttaaa taggaatata agaataacaa cagtgcaata
     8701 tctatataaa tttaactaaa aaagtgaccc attgggtcac tttttatata ttaagattaa
     8761 agtgaaattt attttacagt acacactcat atagataaat atttataata atgaaaaaga
     8821 ggctgaacgt cagcctcttt tcctgtgtga gaaatacgga aaaaagagat gcttgaaatt
     8881 tctttcttcc atttaatatt atactataaa gagaataaaa agtccatgag ttatctttat
     8941 gattaaaaaa aaaaattaca attttgttac tttttagtat acaataataa atttaaattg
     9001 actttgttaa tttttattaa taaaatatcc aaatattaca atatctacag tttattgaaa
     9061 taatgtgtaa attatattat aattggtaaa taaggaaaaa tggtgaaaac caaattttgt
     9121 agggggaaat atatgaatag aaaaaatctt tatgtaatta tggcagctgc aatggtaagt
     9181 acatcagtag ctccaagttt tgctgcagaa gctacacaag taaagaaaca aacaataact
     9241 aaaaaggaag caacagaatt agtttctaaa gttagagaac taatggctca gagatataca
     9301 ggaggttctc aagttggaca accaatatat gaaataaaag ttggtgaaac ttcatcacaa
     9361 ttaaaaataa taactaatat agacgaatta gagaaattag taaatgcttt aggagaaaat
     9421 aaagagcttc ttgtgactat agcagataaa ggacatacaa caaatagtgc aaatgaagtg
     9481 gttgctgaag caattgaaaa atatgaaaat tcagcagacc tttcagctga agctaattca
     9541 ataacagaaa aagctaaaac tgaagctact gggatttatg gagtggcaac tgtagaagct
     9601 tcatatgaca gtacaaaaga taagttagta ataactttaa aagataagac tgatgtagta
     9661 acttctaaaa cgctacaaat aggtattggt gatgaaaagg ttgatttaac agcaaatcca
     9721 gttgatatag caggaaccaa tttagaccct tctgcagaag gatttagagt aagtaaaata
     9781 aataaattag gtgtagaagg agctaaaaat attgctgata ttcaattagc tgaaataact
     9841 ataaaaaata gtgatttaaa tacagtttca cctcaagatt tatatgatgg atatagattg
     9901 actattcgag gtaatacaat agcaaatagt gtaggaaaat caattagtga tatttcatca
     9961 aaagattcag agacaggaaa gtataaattt actgttaaat atactgatac atctggaaaa
    10021 gcgatagaac ttactgtaga aagtactaat gataaagagt taaaagatgt taaagatgcc
    10081 ttaaatggaa atacaaaagt caatttaata gctggggaag acaggtatgc aactgctgta
    10141 gctatagcaa aagaaacaga atatactgac aatatagtta tagttaattc aaataaatta
    10201 gttgatggat tagcagcaac accactagct caatctaaaa aagctcctat attgttggcg
    10261 tctgataatg aaataccaaa agtaacttta gattatataa aggaaataat taaaaaaagt
    10321 ccatcagcta aaatatatat agtaggtgga gaatcagcag tatcaaatac agctagaaag
    10381 caattagaat cagtaactaa gaatgttgaa agattggctg gagatgatag acatacaact
    10441 tcagtagcag tagcaaaagc tatgggttct tttaaagaag catttgtagt aggtgcaaaa
    10501 ggggaagctg atgctatgag tatagctgct aaagctgctg aacttaaggc tcctataata
    10561 gtaaatggtt ggaatgatct tacagcagat gctatcaagt tgatggatgg aaaagagatt
    10621 ggtatagttg gtggttctaa taatgtacct agccaaattg aaaatcaact tgctgatatt
    10681 gataaagata gaaaggttca aagagttgaa ggtgaaacaa gacacgatac taatgctaaa
    10741 gttatagaaa catattatgg caaattagat aaactatata tagcaaaaga tggatatgga
    10801 aataatggta tgttagtaga tgcattggca gcaggtcctc tagcagcagg aaaaggacca
    10861 attcttttag ctaaaaatga tataacaaac tcacaaaaga atactttaga taaaaaacta
    10921 aatcttggtg cagaagtaac tcaaataggt aatggggttg aattgactgt agttcaaaaa
    10981 ataggcaaaa tactaggatg gtaataatca ataatataga aaaggattcc ttattattta
    11041 taggaatctt tttctatcga cataatattc attaaagtat aacatgctct tgacagatag
    11101 gaggatatga tagtgataga agcaacattg tttttttccc cacaccaaga tgatgaaaca
    11161 ttaagtatgg gtagtgcaat aattgaacat gtagaaagaa gcgatacaca tgttatatta
    11221 tgtactgatg gcagtaaatc aataataaga aaagtattag atgatggagg gtcatgttct
    11281 tatcatatta aagatgtaca caagtactct ttatcagaaa gtgatttttc aaaaaataga
    11341 gatgaagaat ttaaaaatag ttgtaaagca atgggagtta aagaaaataa tatacatata
    11401 gaaaataata gagctcatga tggagaattg agtaaggaaa aagcaagaga tataatgcta
    11461 aaatatttag aaaaatatcc tgatgctaaa gtgaagaccg ttacaccata taaagctctt
    11521 ggaatacatg aagaccatag agcattaggt gaagctgcat tagaattgta tagagaagga
    11581 aaaataaaag atttaagatt ttatgtagag ccatacgatt ataatgattt taaaaaagtt
    11641 aatccaaatg ttagagtatg gaaggttttg agtagtcaag aagagaaatt attaaatgct
    11701 atggatgcat acaagaaatg gaatcctgaa aatggtaact acgcaattgg atatcattct
    11761 gtaaaatctc actttgatga attaaaatcc aataaaatcc aatatgtaca tgctccataa
    11821 acattaatat aaaaactagg aggatatagt taatgaaaat atctaagaga ataatgtcgt
    11881 tgttgacagc aacttttttt atctttgcat tatctatcaa tatagctgat gcttcatcaa
    11941 aagagagtct agtgggctct ggtagatggg aaactgcaat aaaaattagt cagaatggat
    12001 ggaaagatgc tactaatgct atactagtaa atgataattc tatagctgat gcattatctg
    12061 caactccatt tgctaagtta aaagatgcac ctattttatt aactcaaagt gataaattgg
    12121 atagtagaac taaagcagaa ttaaaaagac taggtgtaaa aaatgtatat ttaattggag
    12181 gtatgaatac tctaagcacc aatattgaaa aacaattaaa atcagaaaat atgacttttg
    12241 agaggatttc aggtaatagt agatatgaaa catctttaaa attagctgaa agattagata
    12301 aagagaagag tatttctgat atagttgtag ttaatggaga gaaagggcta gcagatgcag
    12361 taagtgtggg ttctgctgca gcacaagaaa atatgccaat tatactttca gatcctaaca
    12421 atggaataaa agttgcaaat agctttatta gtagtaaaaa tataaagaaa tcttatataa
    12481 ttggtggtac atattctgta tctagttcta ttgaacagag tttacctaat gtaaaaagat
    12541 tatctggaaa caatagaaat gaaactaatg caaaggttct agaagaattt tataaaaata
    12601 cagacttaaa aaatgcttat ataactaaag atggtatgaa aaaacagggg gatttgatag
    12661 attctctagc tgtaggtgta ttggcagcta aaaatagctc tccagttgta ttagtaggaa
    12721 attcacttga ctctagccaa tatgatgtgt taaatactaa aagttttgat aatattgtgc
    12781 aagttggtgg aaatggaaat gaagatgctg taaaggatat tcttgattta caagaagaaa
    12841 ctatatatag agttaagaca gcagacgaat tggatgttgc tctaagaaaa gcagatgcta
    12901 atgatattat aacatttaaa cctgatgata ataaaaaaat tactagttca tttagtatag
    12961 aaacaaagaa agctataact gtagagcttg aaggattgta tacacaaaca ataactgtaa
    13021 atatgccaaa tggagaatta actaataagg gtacattaga tggaactgtg aaaataatgg
    13081 atataaaaaa tggcacattt atcaatgaag gtgatataaa tgatattgac atttatgatc
    13141 caaatggatg taaaatagag aataaaagtg gtggagatat atgggtttta actatatcta
    13201 gtagtgctaa agatgtaaat atagaaaata aaggtgatat aagtaaggtt acaaataatt
    13261 ctactagcgt tataataaga aattcaggaa atattgatac tttaagtgga attaatgcac
    13321 cagcaataag tggaaataaa cctagagttg atgataccga taaggatgaa aaaagagcaa
    13381 gtggaatata tcctacttca gaacctttat cacctgcaag agctggttat ataagtgtaa
    13441 aaatattcac taagcctgaa gatacaaatt ataaaattta ttatagagta gtaagcagta
    13501 gaccatctgc attatatatc ggagatagaa tagatacaag tgactggact gctgtaccat
    13561 cttctgtttt accatttact attaaagcag aaaatggata ttatatagaa gctgttgaaa
    13621 taaattctga tactaagaaa gtatctagat gggggaaatc tgactatata gatgatggat
    13681 tttatttaaa atcagaaact gcaaaaggat taagtgcaga tatagatttt gatggagatt
    13741 atgttaaatt aacagcaaca ggtgctgatt ctggatgtac agtttattat agaatagtat
    13801 caaaaaagcc agtatcaatg aatgttggag ataaagtaac tatatctgat tgggatgaaa
    13861 ttactactaa tagagtgagg atagaagcaa acgatgtaaa ggataaatat atagaactag
    13921 ttgaattgga tgattcaact aataaagtaa ctagatgggg agcaagtgat aaggtagtag
    13981 aataggtatc taatgtgaaa cataaaaaca gactgattaa taatgggcag tctgttttta
    14041 tttcctaaaa attatcattt tactaaataa ataatttaaa actataacaa ttatattagt
    14101 aaatactttt acagtaatat catttacaaa aaataaatca atcattatat acataataat
    14161 catttctata ataccagaaa gtagcctaaa actgacaaaa aacaaaaact ccttaaacaa
    14221 aaatattagt tcaattcttt tactctcaaa cacaaaaaac ttattagtaa cataagcaaa
    14281 taaaacagca agtatccatg ccaaagcatt tgcaaccata aaattaaata aaataactct
    14341 agtaaaaaat aaatacgaaa ctatatttac aagagtagta aatgctccaa aaaataaata
    14401 taatatcgtc tctctatgct tttttaaaat caaatataat cctcctaagc taatttaata
    14461 taaatatact taaagatact aacaaacatt agtataacac atctattaat aaatcaatat
    14521 atcttaaaat attataaaaa taaaactata atattctata attaatatgt actcataatc
    14581 catattttat caaaataaaa tatgacctta atttagaaat ctgatataat aaaaattaaa
    14641 tacatataag ggggtaaaca tgagaaagta caaatcaaaa aacttgtcaa agctactagc
    14701 attgttgaca gtttgctttt taatagtgtc aacaatacct gtctcagcag aaaaccataa
    14761 aactctagat ggagtagaaa ctgcagagta ttctgaaagc tatcttcaat acctagaaga
    14821 tgtcaaaaat ggagacacag caaaatataa tggagtaata ccttttccac atgaaatgga
    14881 aggtacaaca cttcgaaata agggtagaag tagtcttcca tcagcatata aatcaagtgt
    14941 agcatacaac ccaatgaatc ttggtcttac tacaccagca aaaaatcaag gaagtcttaa
    15001 tacatgctgg tctttttcag gtatgtcaac gttagaagca tatcttaaat taaaaggata
    15061 tggaacgtat gacttatcag aagaacattt aagatggtgg gcaactggtg gaaaatatgg
    15121 atggaactta gatgatatgt cagggagttc aaatgtaaca gctataggat atctgacagc
    15181 atgggcaggt cctaagttag aaaaggacat accatataat cttaaatctg aggcacaagg
    15241 tgcaactaaa ccttcaaata tggatactgc acctactcaa tttaatgtaa cagatgttgt
    15301 tcgtcttaat aaagataagg aaactgtaaa gaatgctata atgcaatatg gttcagtgac
    15361 atcaggttat gcacattatt caacatattt taataaagat gaaacagcat ataactgtac
    15421 aaataagaga gctccattaa atcacgctgt agcgatagta ggatgggatg ataattattc
    15481 aaaagataat tttgcatctg atgtaaaacc agaatcaaat ggagcatggt tagtaaaaag
    15541 tagttgggga gagtttaatt ctatgaaagg attcttctgg atttcttatg aagataaaac
    15601 tctactaaca gatacagata actatgcaat gaaatcagta tcaaaaccag atagtgataa
    15661 aaaaatgtac caacttgaat atgctggtct tagcaagata atgtcaaata aagtaacagc
    15721 agcaaatgta tttgatttta gcagagactc tgaaaaactt gattctgtta tgtttgaaac
    15781 agactctgta ggagcaaaat atgaagtata ttatgcacca gtggtaaatg gagttcctca
    15841 aaacaattca atgacaaaac ttgcaagtgg aacagtatca tattctggat acataaatgt
    15901 acctactaat tcttacagct taccaaaagg taaaggagca atagtagtag ttatagacaa
    15961 cacagcaaat cctaatagag aaaaatcaac tttagcatat gaaactaata tagatgcata
    16021 ttatttatat gaggctaaag caaacttagg tgaaagctac atacttcaaa acaataagtt
    16081 tgaggatata aatacatata gtgaattttc tccttgtaac tttgttataa aagctataac
    16141 aaaaacatct tctggacaag ctacttcagg agaatcttta actggagcag atagatatga
    16201 aacagcagtt aaagttagtc aaaaaggatg gacttcttca caaaatgcag tattagtaaa
    16261 tggagatgca atagtggatg ctttaacagc tacaccattt acagcagcaa ttgactctcc
    16321 aattcttttg acaggaaaag ataacttaga ttcaaaaact aaggcagagt tacaaagatt
    16381 aggaactaaa aaagtttatc taataggtgg agaaaattct ttaagcaaga atgtacaaac
    16441 tcaacttagt aatatgggta tatcagtaga aagaatttca ggtagtgata gatacaagac
    16501 cagtatatct ctagctcaaa agttaaatag tataaaatct gtttcacaag ttgcagtggc
    16561 aaatggtgta aatggacttg cagatgcaat aagtgttggt gcagcagctg ctgataacaa
    16621 tatgccaata atacttacta atgaaaaaag tgagttgcaa ggtgctgatg agtttttaaa
    16681 ttcatctaaa ataactaagt cttatataat tggtggaaca gctactttat catcaaattt
    16741 agaaagtaag ctttcaaatc caacaagact tgcaggcagt aatagaaatg aaactaatgc
    16801 taagataata gataaattct accctagctc agatttgaaa tatgcttttg ttgttaaaga
    16861 tggttcaaag agtcaaggag acttgataga tggactagca gtaggagcat tgggtgctaa
    16921 aactgattca ccagtagttc tagttggaaa taagttagat gaaagtcaaa aaaatgtact
    16981 taagtctaag aaaatagaaa ctcctattag agttggtgga aatggaaatg aaaatgcttt
    17041 taatgaacta aatactcttt taggaaaata gtgaatggaa agatagtaag gatatacatt
    17101 ttattattaa aaaatgttca atagaagttt ataaatataa aataacaaaa aggggtgtct
    17161 caaaatgaac tttttagttc atgaggcact cttttttgtt gttttatatt tttatcattt
    17221 aaataatgaa aaataggatt gtctatattt ttagatagtt ttcttattat tgatgaaaaa
    17281 tgtaataatt ttaaactgta aaatagtgta aagttatttt ataatataaa tatagtaaga
    17341 ttttaaacaa aataaggggg aataataatg aaagcaccaa aaactatttt aacaatactg
    17401 acaatagcgc ttactttaag tagcatttct ataataccat catatgcact tacagaggaa
    17461 aaattaatag gtaatggtag atatgaaact gctgtaaaga taagtcaaaa ggcatacagt
    17521 tctagtccta atgtagtgct agttaatgac aattcattag ctgatgcact ttcagctaca
    17581 ccatttgcaa aggctaaagg agcaccaata cttttgacag aaagtgataa gctagatgat
    17641 aggactgaaa aagagataaa acgtctagga gctaaagata tttatttgat aggaggtact
    17701 gctgtactta ataaagatat agagaacaag ttaaaaagta atggacttaa tgtagaaaga
    17761 attaatggta aggatagata tgaaacttca ctgattttgg ctaataagct taaggatata
    17821 aaagatatta aggaagttgc tgtagtaaat ggtgaaaaag gattgagtga tgctgttagc
    17881 gttggggcac cagcagctca aaataagatg cctataattt tgtctagtcc taaggatgga
    17941 gtagaagcat ttgataaatt tataagggat gagaaagtta taaaagcata tgtaattgga
    18001 ggtacaaatt ctgtttcaag agctgtagaa aagagtcttc ctaatgcaga aagagtgagt
    18061 gggaaagata gaaatgagac taatgctaaa gttatagaaa agttttacac tgatacaaat
    18121 ttaagtaatt tatatgtaac taaggatggt agtaaaaatg agaatcaact aatagactcg
    18181 ttagctgttg gcgtattggc tgcaaaaaat gagtctcctg ttgttttggt tggtaataaa
    18241 ttaaacacaa aacaaagaga tatacttagt actaaaaaat taaatactat aacacaggta
    18301 ggtggaaatg gaaatgaaga agcttttgat gaaattaaaa gtctacaaga aaaaactgtg
    18361 tttgaagcaa aaactgttga ggaattaact gatatgataa atatagcaag tcctaatgat
    18421 attataaact ttaaaccaaa agagaatact gtaaatgaag cttttagaat ggtaacaaat
    18481 aaaccaataa ctgtgaatat aaagggtgat tgttcaaaga ctcttacagt agatatgcct
    18541 aatggtgaag tgaataacta tgctactttg gtaaatgtta tagttagaaa tattggagaa
    18601 ggtggattta ataatcatga tacaatcact atattgtcag ttcgtgacaa aaatggtaga
    18661 gttatagaaa atactagaaa ttcagatata gatacgttga tgatattagc ttctgctaat
    18721 gatacaaaat tgattaatga tggatatata ggaaaactta tagataattc atctaatagt
    18781 gatataacta ataatggtac tatagataaa aaagttaatc aggttgaaga tttagaggct
    18841 aaagtagatt caattgaaaa agctatagat tctataagtc aaaaagtcaa taaaatacaa
    18901 gatatactag ataagcttgg atttctaaag aaatttctta gctaagaata tgtatattta
    18961 agtgaagtgt gtgttgtaat tgttctaaag ttatgtagtt aaacaggttt atatacaaat
    19021 agatttatag ataaatatgt agtttagtat atatttgaat aagctttggg gaggtaacat
    19081 ggggattgga attgaatgta acaaaaaaat aaataaaagt attgacaaaa ctagaataaa
    19141 gaaaaataat ct
//
LOCUS       DAMKMI010000072        19038 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMKMI010000072 DAMKMI010000000
VERSION     DAMKMI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19038
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.073"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            72..572
                     /locus_tag="PC429_002261"
     CDS             72..572
                     /locus_tag="PC429_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3847762.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(822..1601)
                     /locus_tag="PC429_002262"
     CDS             complement(822..1601)
                     /locus_tag="PC429_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847763.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1598..2077)
                     /locus_tag="PC429_002263"
     CDS             complement(1598..2077)
                     /locus_tag="PC429_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3847764.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            3063..4070
                     /locus_tag="PC429_002264"
     CDS             3063..4070
                     /locus_tag="PC429_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF3847765.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            4108..5535
                     /locus_tag="PC429_002265"
     CDS             4108..5535
                     /locus_tag="PC429_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF3847766.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SAGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            5677..6942
                     /locus_tag="PC429_002266"
     CDS             5677..6942
                     /locus_tag="PC429_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3847767.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTRIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            6984..8582
                     /locus_tag="PC429_002267"
     CDS             6984..8582
                     /locus_tag="PC429_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF3847768.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            8617..9639
                     /locus_tag="PC429_002268"
     CDS             8617..9639
                     /locus_tag="PC429_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3847769.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(10027..11430)
                     /locus_tag="PC429_002269"
     CDS             complement(10027..11430)
                     /locus_tag="PC429_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3847770.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            12297..12806
                     /locus_tag="PC429_002270"
     CDS             12297..12806
                     /locus_tag="PC429_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF3847771.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            12834..13028
                     /gene="rpmI"
                     /locus_tag="PC429_002271"
     CDS             12834..13028
                     /gene="rpmI"
                     /locus_tag="PC429_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF3847772.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            13084..13440
                     /gene="rplT"
                     /locus_tag="PC429_002272"
     CDS             13084..13440
                     /gene="rplT"
                     /locus_tag="PC429_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF3847773.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            13750..14295
                     /locus_tag="PC429_002273"
     CDS             13750..14295
                     /locus_tag="PC429_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847774.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            14282..14446
                     /locus_tag="PC429_002274"
     CDS             14282..14446
                     /locus_tag="PC429_002274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847775.1"
                     /translation="MKGNKSDLHEVEPIYTENRFMIYICIQSKVSLLGLYFIVSFHLS
                     NDLEWYNEVF"
     gene            14735..16660
                     /locus_tag="PC429_002275"
     CDS             14735..16660
                     /locus_tag="PC429_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3847776.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKYTEIKILGTGDL
                     HSILTDSMVSYVNEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFVSNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDKLTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWIINDKFSKTIEVE"
     gene            17061..18965
                     /locus_tag="PC429_002276"
     CDS             17061..18965
                     /locus_tag="PC429_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3847777.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSSLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSSSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         7198 a         2140 c         3380 g         6320 t
ORIGIN      
        1 atagcattat aaaatagaat catataaaat aagttaacta caatcaaaaa ttaaaaaggg
       61 gaaatgttaa gttgatagaa ggcataaaat ttataaaggc agaagaaaag tatatttatt
      121 cgtattggca aacttttgat aaaatagcaa aagagaggaa atatctagct atggatgaag
      181 cctttccttt tgaagaaaca gttgaattta taaaaaatat tataaataaa aacttaccac
      241 aactgtttat aattgattta gaatcagata actgtattgg ttggtgtgac gttttaccta
      301 aaactgaaaa agttggttat ttaggcatgg gtattcttaa agagtatagg gaaaaaggta
      361 tcggaagtaa tcttttaaaa caaattattg atttatcaaa agaatatggg tatgaaaaaa
      421 ttgagcttga tgtatttaaa agtaatagta gagctattca tgtttataaa tctttagggt
      481 ttgtagaggt aaatacaata tctagtggtt ttacatggaa tgatagacct gtaaaagaag
      541 aagtaattca aatggagctt acacttatat aaaataaagt taaagtcttt aaaattccat
      601 atacacttgt ataaaataaa gttaaagccc ttaaaattcc atataaaaga aatattttta
      661 agggcttttt aataatgctt cttttgaagg tagctaaata tctaaaattt tatatattaa
      721 atttaaagaa aatataaaga tggaagatac aaccatgttt actaataaaa aaattaaagt
      781 cataaataat aaactcataa acaacttatt attacttatg tttattttgc atctattact
      841 tcatgagcca ttttaactaa gtcattcata gataaatttg agtcttgaac tgtaaaagaa
      901 tagtaaactc catcttgttt ccatgtcaaa aacttaaatt tattaacttc aactttgtct
      961 gattcattat ttggccctac actaaatgtg tatttgcctg atttttcatc aattttatcc
     1021 tgttctgtca ttttataacc ttctggtaca aacttttgtg cgaatgcttc ataagaaata
     1081 ttaatatctt tgtaagtatc tacaactttt agattgtcag aactcttccc aagtacatta
     1141 tttgctgtgt caagagatat ttcatcattg ccatttttat attcaaaact aagtgctttt
     1201 gattttccta aaacattatt ttctttgtca aatgcttttt tatttacagt atatgctcct
     1261 ttaaatttat atccattact aaattcagaa actatattag gggtaaagtt aaagtctttt
     1321 tttacttgtt ctgtagttgg gatagatgta taggtagcct tagtacttga ttttccaacc
     1381 aaactatata tatgtcctac tgcaagggct gttgtgctca atatcaaagt aatggctaaa
     1441 gttgcaacta agccttttct aaatggtaat ttgtgtgttt tattattaat cattttataa
     1501 ttttcctctc tttctggtaa actatttagt gttttgctta aactattatg aaaactttct
     1561 ggaacttctg gaaacgaatt atttaatata tttttattca tatgattacc tccgtgtttt
     1621 ctaatttagt ttttaataat cttcttcctc ttgataatct actttttaca gttccttgag
     1681 gtatatctaa tatttcctta acttcatcta ctgaataacc ttcaatatag taaaggacta
     1741 ttggaatttt aattttttta ggtagacttt gaatagcagt atacaaatcg ctatagtcat
     1801 ctttttcatc aatttttttg tttttgaaga catcttcaaa tgatagagat tttaatcgtt
     1861 tgcgtcttaa tgaattacat tcatttatga ctattctaat cagccatgtt ttaaagtatt
     1921 gttcttcctt aagagaatcc aacttttcat atgcttttaa aatagcattg ttgacggcat
     1981 cttcacagtc ttggtcgttt cctagaatag acttagatac tcgatacaaa gtctgttctg
     2041 attcaagtat attgtttgta aatgtagttt tatccaacta agttccttcc cttctatgca
     2101 aatcgtagcg ctactttttt gtctttcact tattagataa aattttacta tagatggttc
     2161 acttttttta tgattatttg ataaatttaa gtttgaaagt tttgcctatt ctatacaaat
     2221 attctataat atattttttc tagtattttc ccctcatata taaagccatg tagatatatt
     2281 aaactttaga agccatttat atatttagtt ttatagtaag tctttatatt gagtaattag
     2341 aaaatagtaa gtatttaatg ttgagtagtt agaataacaa tgcttttgga ttatttactt
     2401 catgtacaga tttaaaataa gttctttaaa tagcaacttt ttgaaaaaac gcaaaaatat
     2461 tttatgttgt ctgattatat ttatagatgt gaaacaatat tgtatagagt aaaagtatat
     2521 agaagatgat tacatagagt ttatacttga attttacaaa tataattatc aaaatactgt
     2581 caaagagaaa aatagttttg atgaacgttt ttatcaaatt gctacagaga cagggctaag
     2641 tgctaatact gtatataaag tattcaataa aataataaat agtggaaaaa ctgaatttac
     2701 atcaaaggaa ttagctacta tatgtggtgt aagtattaga actatggaca ggattatact
     2761 tagattgtgt gattctggtt attgtgaggt agtgagtgaa cagcttatga gcaagtatgg
     2821 tagacccagt agaatattac gttttaaacc ttttctgttg ttttaaatag atataagtca
     2881 aaaattaaat ttttatgata acattttctt tacaattttt tagatatatg ttaatattta
     2941 gaaaaatgca tatgtattca taaaataaac ttaaaacagc taaaaaatca ttaaaaagga
     3001 aatcgtatgc atgaaatgag aaagttaatt tataacttaa ataaatctgg gaggagataa
     3061 ttatgggaaa atatcaggat tcaaaaaaag ttgtaagaaa ttattttgaa gcattagaaa
     3121 atgctactgg ggatgaggtt gaaaatgtct taaagcaaca tatgaaatca gattataact
     3181 ggaaaggtgt gtatccattt cgtgaacaag aaggcactga aaatgtagca gatgtatttt
     3241 ggaagccttt aaaaaaatca ttgagtaata tgcaaagacg acaagatatt ttcattgcag
     3301 gaacaaatga gattgacaat agtgaatggg taatgagtat ggggcatttt atgggacttt
     3361 ttgataacga ttggcttggc attaaacata ctcgtaaaat gattagttta agatatgcag
     3421 aatttaactg tgttcaagat ggtaaaattg ctaaaactgg attatttgta gatataattg
     3481 ggtttatgat tcaagcagga gttaatccat tgccaccaca aacaggttca tattttgtat
     3541 atccagggcc aattgaccat aatggattac tttttgaaga tgcagatgaa gttgaaggag
     3601 taaaaacttt agcattagta aataaaatgg tagatgattt atctgaatta aatgaaagtg
     3661 gtgctatggg atgcccacca gaaatacttg aaaaatcttg ggctaaagat atgatttggt
     3721 atggtccagg tggaattggt gcttcttata ctatacctag atatcaagag caacaccagc
     3781 taccatttag aaataattta aaaggaaaga ctttcaatgg gcatgtatgt agatttgcag
     3841 aaggtaattt tgcttgtttc tttggatggc ctaatctgac taatgtacca tgtggaggtt
     3901 ttttaggatt accaggtgga gaggttaaag cagatatgca agttgttgat gtttattatc
     3961 gtaagggcga taaacttcaa gaaaactggg tgttaattga tattccttat tggttgaaac
     4021 aacaagggct tgatattctt gagagaacta aaagtatttt taatgcttag ttatgcatac
     4081 gtattctaaa ataaaggggg atataaagtg gcatctacca acaaaaataa tgcaacaagt
     4141 aaagaagaag aaaaagaagt ttctacaagt gggcttgaat catgtacggt taaagaaaac
     4201 aaagctaaaa tgaatcaaac taattttagt aatataaatg aaaatcaaac aaatgagaat
     4261 aaaacgaaca catataaaag aaatatagat aaaggaaata tagataaagg gaatatagat
     4321 aaaacaaaca caaataaatc cgacataaat aaagttaaca tagatacagt ggatacagat
     4381 aaattaaata caaatactct tactaaaaaa aataaaaaag ttatatttaa gggggaagat
     4441 aatatgtcat ctacaaaaat ttactatgga gattcattag aagtaacgcc tgttggagta
     4501 atggattaca atgatttttc aaaacagaca aaaagggaac aggaaatacc tggatttgat
     4561 tctaagtata gagattttgt agattatatt atgaagatta cacataatat ctgggaagaa
     4621 aaaggtatag gtgttattta tgatacatat cacaataatg ttacaatgca ttgtggctct
     4681 tcaaatttag ttggtattaa agatgttatt tccaatactc ttcaaacatt acatgcgttt
     4741 cctgatagaa gattaattgg tcaaaatgta atatggtcta actttggtgc tgatggtttt
     4801 ttatcttctc accgtgtttt gtctacagca actaacttag gtgatagtaa ttttggacca
     4861 gcaacaggta gaaaaataaa ctttagaact gtaattgatt gtgccacaac taacaataga
     4921 atccacgaag aatggttggt tagagataat ttatggattg ttactcaatt aggattaaac
     4981 cctcaggaag tagcaaagag tatggcaaag gcaagtgaat ctaaagtgct tagtttacaa
     5041 tcaacttatg gtatctgtga atctatggat ggtcaattta tgccaactaa gtatcaagca
     5101 aaagatgatt ctgctggcga aatgatgctt gaaatgacaa gccgtattta taattataaa
     5161 tatataaatg aagttaaaaa atactatcat gataatgcag tagtacattt tatatgtgat
     5221 aaagatttaa atggttatga tgagattcaa ggaatgattg taagtttact tgcatcaatt
     5281 ccaaatggaa gttatgaagt ggaaagagta acttgtaatc aaagagaaaa aaatgaaggt
     5341 tatgatgtat ctgtaagatg gagattacgt ggaataaatg aaggtatagg attttttgga
     5401 cagccttctg gaaagcatat agaagtaatg ggaatcaatc attatcatat actacaaggt
     5461 aaagtaaagg aggagtggat aacttttgat ggtatggatg tattaaaaca aatgtatatg
     5521 ggagtagaag aataaagctg tttagagtat aaatagtttt gaaatatata gggaagtatt
     5581 gctttataaa atttaagtgg ggaatgtata tgaaaaatag atgtttaaac ttttagcaaa
     5641 attatgacta aatttaaaag ggggaattat aaaaatatga aaacaacggt tgaaatagaa
     5701 aatgatatca tattaggaaa agactattca aacctaaaaa gaggaattgc atttttttca
     5761 attgctttaa tatacttttt ttattgctac aattttatgg tgggtacttt tattaaacca
     5821 acaatgattt atgctttagc agatggggga tttggttttt cattaaaaca aacagaagaa
     5881 atatttgcag ttatgtcttt tggtacaatt ccaggtacat ttatttttgg ggtgatttct
     5941 acaaaaattg ggaagaagag gactttaatt agcgttgctt tactgattgg attgacaaca
     6001 tttataccaa tgttatctcc tacaaatatt atgttatgga aaatagcacg tttgtgtaca
     6061 ggagtagtgt tgggaggcgt atttggaaca gctatgcctt tagttgcaga tatgtttcca
     6121 agtaaatatc gtggtaagtt agctgctata ttaactgctt tattttcatt agcaatgatt
     6181 tttggtggga aagtatatgg cgttttaggt gatgcaaatt ggcaaatatt gatgtatact
     6241 gcgattatac cacctatagt tggagctatc ttagcaattt tctttgtgcc agatgatttg
     6301 gagtatacaa aggaattagt taaaaaggga aaagaaactg gtgaaaagat tagctatata
     6361 agtatgtata agggaaaata cttatggatt ggattgggaa cgattctttt atcaggtgca
     6421 aattttacag catattcagc attttcaaat aatgcaacta catacctagt gacaagaata
     6481 ggaatgtcag cagcagtggc tgggtctata tatagtttac aaggaattgg tcaattagta
     6541 ggatatttat tctggggttc aattgcagat aaatttggaa gaaaaatacc ttttataggt
     6601 atggcattag cagcagtatt tgtttttata tatacaaaat taggtggaga taatacaaca
     6661 acattctata tgatatcagt attactaggt gttgctgttg gatattctgg agcatgggga
     6721 gcatactaca cagagttgtt cccaagtaaa tatcgttcgt tgtcatctgg tatgtcattt
     6781 aatggaggaa ggattatatc tacgtttgca atacctgcta tagctggtac agcttctggt
     6841 actttaggta tgatgactat cttttacata tcaatcgcag tctttgtagc aggtgctatt
     6901 gtatggttat tcttaccgga gacgttaaat aataaaaaat aaaatactag gaatttattt
     6961 tgttacttag aaaggggtgg cttatgattt catcaattga tgtatgtatt ataataggat
     7021 atttgctatt aatgttagtt attggttatt attcaggaaa agataataaa aatcaagaag
     7081 attatttctt ggcaggtagg tcaatgccat ggattccaat tgctttatca gttgcagcta
     7141 ctatgattag tgcaaatggc tttataggtg gtcctggatg ggcatatgta gatggaatgt
     7201 atccagtaat ggttaatatt acagtacctt tagcaatatt ttttgcactg agcattacca
     7261 ccccagttat ctataaatta aaaataacct ctatatatga atatatggga cttcgtttag
     7321 gaaattattc aagaggatta actattattc aattttttat taattcattg attcaagcta
     7381 gttctatggt atatattcca gtgcttatta ttcaaatgat tactggctgg tcactttatg
     7441 ctttagtccc aatagttgta cttgtatcaa ttatatatac tctattaggt gggattaagg
     7501 cagttatatg gactgatagt attcaaatga tagtagtaat aagtgctgta tttatagtaa
     7561 ttattgtagc cttaaaagga atgaatctaa gtttctttga tacattacaa attgcacaac
     7621 agagtggaaa attaaataca tttgatttca gtagggatat ttcagtgaca aatacttttt
     7681 gggctacttt aattggtgga actgtaatgt ggataagata cttttgtttt gaccaatctc
     7741 aagtacaaag agttttaaca tctaaatctt taaaatgtgc aaaaaattct tttgtagtta
     7801 gtgcatttgt tatgaattta gtttactact ttatgctatt ggtaggagta attttatttg
     7861 ttttttatgg gggaaggaca tttgaaactt caaatgaaat aatgattact tttattttaa
     7921 atgaacttcc agtaggagcc attggaatta ttattgcagg tgtgtttgca gcagcaatgt
     7981 ctagtatcga ctctatatta aacagtatga caactgtttt tacgaaggat atttatgagt
     8041 tttatttcaa aaaagacaac aaagaatcat cattaaaagt tacaatgatt ataactgtag
     8101 ttataggagt tgttatgaca ggttttatta ttatgggatt tggaggaagc atcaaatcaa
     8161 ttcttgacct tgttggtaac tatatctcat actttgctgg accagctaca ggtgcatttg
     8221 tactagcaat gtttacatat aaagctaatg ataaaggagt atctattggt tttattgtag
     8281 ggttagtttt aggatttttt atttctaata aatataatgt gagttggttg tggaatccag
     8341 caataggtgc tacaataaca ttgatatttg gatatatttt tagtgtcatt ttaaagaaaa
     8401 atcataatgc agatgatatt aaaaaataca ctgcatttgg tatgagaaat caccttattt
     8461 ctcaaggaat ggataaagta gatggagtat ctatagtgcc attttcttta ggtaaacaag
     8521 aactaattgt attagctttt tttattatcc aatatattat attatttttg atacaatttt
     8581 aagaagaaat actagatatt taaggagaaa tactaaatga atcaaaagaa agttcattat
     8641 aataaatcgg ctacctcaaa agatgttgca agattggcag gagtatctca atcatctgtt
     8701 tcaagagcat ttggtagtgc aaatggaaag ggcgtaaaac ctgaagtaag agaaagaatt
     8761 tttcgtgtag caaacgaaat aggatatgtt cctaatttgg tagcacgggg aatgattagt
     8821 ggaaaaacta atgttatagg attgatagtt ggtgatagtc taggtccatt ttacaataga
     8881 attatcaatt tatttgtaga aaaaattcaa gagataggta aacagtgtct cgtttttaaa
     8941 gttcctagac aacaaagtat agatagtata atttcaaaag taattcagtt tcaagtagaa
     9001 gctgtgatta ttacagcttc ggccatgaat aaggtgatgg ctgagacttg tgaagaaaac
     9061 aatattcctg tgattctttt taatcgtttt atacctggta taaaaataag tacagtctat
     9121 gtagacccaa ttgaaggtgg gggaatggct gctgaatatc tgcttaagaa aggtcataag
     9181 aatataggat atattcaatt tacgagagaa acaagtgaag aaatggaaaa gaaaattgga
     9241 ttttattcta agttaagaca aagtgggatt cataacctta aagaagaatc tgcaaactat
     9301 gattatgatt ctggatatga agcaggtaag agaatgttag cattatcaaa tcctccaaca
     9361 gcaatttttt gcacaagtga cttgatagct attggtgtaa tggatgctgc aaggtttgag
     9421 tacaagttga gggtgccaga ggatttatca gtgataggat atgatgatat tcaaatggct
     9481 tcatggaaga gttataattt aacgacgatt agacagccac tagatttgtt gattgaaaaa
     9541 acaattaaca tacttaagtc cttattaaat gaagaaaatc cagagtcagt tgttgaaatg
     9601 ttaaaacctg aattgattga aagaggttct acactttaag tggtagatat ttaattaagt
     9661 ggtagatacc taattaagtt gtagatatct gataagaaat ttttaagttt tcaatcgttc
     9721 taaattgtga gtataaaaat ctactaaatt tagttatgta aaatataaat tataagtgta
     9781 gaaactaggc tactagcttt tataccactt caaacattac tggaggtagt taagtttaca
     9841 tcagtttagt tttatgtaga tataatattc tgtaaataga caaaattaaa atatgtaagt
     9901 gtctcaaaat gaaattttta gtttatgagg cacttttttg tataaaatca aatgtatttt
     9961 tataacttta acaataaaaa gaggcttatc tctattttga gacagcctca aatttttaaa
    10021 actatattag tttacagctt cttttcctat caaatctttt aactcttcat gagtcatagt
    10081 ttctttttca aataagaatt tagaaacaga atgtaaatct tcaagattat cctttaacat
    10141 ctctaaagtc tctttataac aaatttcaac tatacgattt gcttcctttt gaaccttatc
    10201 acataagaaa gtcttatcat ttccatcaat agtcataaat cctaagttac tcataccata
    10261 ttcacaaacc atttggttag ctatttcagt gactttcttt aagtcgtcct ttgcaccagt
    10321 agatttatga ttaaatatta attcttctga agcctttcca gcaagtaata tttttatttt
    10381 tccaatcaac tcatccttag tataaatata tctatcttca tcaggtaatt gaagaacata
    10441 acctaatgct tgtcctctag gaactataga tattttttgt ataggacaaa ttcctactat
    10501 atcacttaca agagcatgtc cagcttcatg atatgctact atttttttct ctttttcaac
    10561 caatgcagag tttttagatt ctagccctgc aataactctt tcaagtgcct tgtcaaaatg
    10621 ttctgatgta atacactcac tgtcatctct aactgcaaat atagctgctt catttgctat
    10681 atttgaaaga tgagccccat tgaaaccatg agttttttta gctaaaagtt ctagatttac
    10741 agatttgtct ataggtttgt catctgtatg tacctttaga atttcaaatc tagtgtgata
    10801 gtttggtgca cctatatgaa tgtgtctgtc aaatcttcct ggtctaagta aagcttcatc
    10861 taataagtca agtctattgg tagcacctat tattaataca ttggaatctt tattaaatcc
    10921 atccatttca actaaaagtt ggtttagtgt ttggtctttt tcgttgttac tttcaagatg
    10981 tctctttgct cctacagcat ctatttcgtc tataaatatt atacttggag cttcttttct
    11041 agctttttca aatagagttc taactctttt tgctcccaca cctacatatt tttcaacgaa
    11101 ctctgaacca gtaacattga aaaatgatga gtttgtttca ccagctacag cagatgcaag
    11161 tagggtttta cctgttcctg gaggaccata aaataatact cctttaggta tttttgcacc
    11221 cattttttga tatttttgtg gtgacttcat aaaatcgact atttcaaata gttcttcttt
    11281 tacttcatct aatccagcaa catctctaaa tgtagtcttt ggttttgtag aaagtgaatc
    11341 atctgtttga ttatctcttt ccatttttgt ctgtgcaaag acaggagctg gattgttgat
    11401 tttcttaaaa aagttgttta gcattttcat attcacacct caggtaatta attattgtta
    11461 atcaatttta tattttccat atttactatt ttttattatt tatttttcat ggaaaaaata
    11521 tacatgttat ctaaataaaa ataaatttgt aagatgtatg aaaaaatgaa tttatgtata
    11581 tagagatttc tacttatgta ttgaataaag tattacaaat tataaattat gtattgatta
    11641 tatattgagt aaagtattat aaagcatgta ttgattaagt attgaataaa atattacaaa
    11701 tttagttgaa atgaaaatga taaaaaatct tgtactatat tttacaaatc atataaatag
    11761 agaaattgtt gtagcacaaa gtagtaggta gtatattaga atataatata ggatttgttg
    11821 tgtagtatag tagcatatta gaatataata taggatttgt tatgtagtat agtagtatat
    11881 tagaatacaa tatagaattt gttgggtagt atagtgttat attagaatac aatataggat
    11941 ttgttatgta gaatatatag agtcatgtag aatataaata tataaaagga ttttgggtat
    12001 aaatttaaaa attaatagca aaatgagtag tatagacata taattagtag taggaagtaa
    12061 ttaaattttt cctaaacaaa aaaaatagtt gcaaataatt tgattaagtg atatagtaga
    12121 taaggtaaga ataaaacaaa gaagaagtcc gcttctcacc ttacgccgga ggttagtaag
    12181 gtaaatataa gttaaagctt aattttaatt taagtttgtt taaatttggt aacttatatt
    12241 gcggatgatt acgtcatccg ctttttattt tataaaaatt ttggaggtgt cctacaatta
    12301 gcaaagaatt agcaatcaat gaacaaataa aagataagga aataagagtc ctttcaccaa
    12361 caggtgaaca acttggagta atgcctacta aagaagctca agcaatggct aatagcaaaa
    12421 acttagattt ggttcaaatc tcgccgaatg caaatccacc agtatgtaag ataatggatt
    12481 atggaaagtt tagatatgag caagcgagaa aagagaaaga agctaagaaa aaacaaaaaa
    12541 ctatagttgt aaaagaagta agacttagac caggtattga acaaaatgat ttaaatacga
    12601 aagctaataa tgctataaag ttccttaaaa aaggggataa agtcaaagtt gaacttagat
    12661 ttagaggaag agaattagga cataaagaca taggtaaaga agttatgctt aagtttttag
    12721 atataataaa agagtttgga gaaccaacaa aagcccctgc atttgagggt aataatatgg
    12781 ttgtaattat agatccaaaa aaatagatga ttaactgaga ggaggaattt gtcatgccta
    12841 aaatgaaaac tcatagaggt gcagcaaaga gattaaagaa aacaggaact ggaaagttaa
    12901 agagagctaa agcttttaaa aaacatatat taactaaaaa atctgcaaaa actaaaatga
    12961 acttaagaaa atcaacttta gtaagtgatg gagatgctaa gagaatagca caattattac
    13021 catactaaga tacagattgt aaataataac tatagaagag ggatataaaa ggaggtcata
    13081 acgatggcaa gagttaaaaa agctatgaac gctcgtaaaa agcataagaa aatattaaaa
    13141 cttgcaaaag gattcagagg atctagaagc aaattatata gaccagcaaa tacattcgta
    13201 atgaaagcat taaaaaatgc ttatatagga agaaagttaa agaagagaga cttcagaaaa
    13261 ctttggatac aaagaataaa tgcagctgct agaatgaatg gaatatctta ttcaagatta
    13321 atgaatggat taaaattatc tggtattgaa gttaacagaa aaatgttatc tgaaatggct
    13381 atacaagacc cagaaggatt tgcaaaatta gcagaagttg ctaaagcaaa attagcttag
    13441 tattgaatca tgagatgtaa agtctttgag ttatataaat aattcaagga cttttttatt
    13501 ttttgaaaaa tacttattag tgtcatgtac aaccaatttt ataatgataa ataaaataag
    13561 caatacaata gaattttatg agatttatta taagaattgt tacaattatt tatgatagct
    13621 ataaaaagtc atagtaaaaa tgatattgat gtatgttgat atatgtataa agctttcaat
    13681 caatcttgaa ttaatattat caacatgata atattatctt attaataata taaataaggg
    13741 ggagataata tgaatgaaaa actaatggat tgttttgtaa acccaattaa atgcaagtta
    13801 ttgattgaaa ttttttcaaa taatcgaact acagctaaac aattaggaga aaaatttcaa
    13861 gatatatcaa aggcaacact ttatcgttac cttaataaaa tgttgaaaga tgatattata
    13921 aaagtagttg aagaaaataa aataagggga actgttgaaa aagtatatgc tatgaagtta
    13981 gatatagctg atgatgtgaa caaaattata gatgagaact ctggagaaga atatgctcaa
    14041 ctgtttactc aatatatgat gggattttta agagaatttt atgaatacgc tgaaagaaag
    14101 gaaaaggata tcctaaaaga tggttcagga ttttctgtct gtccagtata tgctagtatt
    14161 gatgagatag ttgaagtgag taaaaaaatt gctagtatac tagaacctct aagaaataac
    14221 tcgcacgaag aaggcagaag aatgcattct ataggagtga taatcactcc acctaaagag
    14281 gatgaaggga aataaatctg atttacatga ggtagagcca atttacacgg aaaatcgttt
    14341 tatgatttac atatgtatcc agagcaaagt atctctgctt ggattgtact tcattgtatc
    14401 atttcatcta tctaatgact tggagtggta caatgaagtt ttttaatttt tcatatcaat
    14461 gcctaaagaa atgaacttta gaccacatat gcaattaggt tacattatac aattagacca
    14521 gatatacaaa ataaaaatca gtatttttat agcagataaa ttgactgtta ttcttcttaa
    14581 aaatcatcta ttagttattg ttagcatatt gaaagaaatt ataggaatct attgaataaa
    14641 tgaatattgc ccataatcat ataaactgct atagtttaaa ataaagcagg acgttaaatt
    14701 ttatagtaat ctaaaaattt aagaggggga ttttatgaaa ataaaatttg ttattatgcc
    14761 attggtgata tttttaatgg tattatttgt ggggtgtcaa agtacaaaag atacagaagt
    14821 taaagcaaaa tatacagaaa ttaaaatatt aggtacaggt gatttgcatt caattttgac
    14881 agactctatg gtttcatatg taaatgagga aagggaaaag aataaaaatc tgcttatggt
    14941 agatgcggga gatttctatg gtacacaaag tagagaaatg tgggaatggt cttctggaaa
    15001 aaagttaatt aatattaaaa gaaatggtag agcagagtat gatgacataa ttaagagtag
    15061 taaagatgaa gtacctatag ttaaagatat ggcaaagttg aaatatgatg ctgtagtttt
    15121 gggagataat gaatttgttt caaatgataa gcaaagtctt gataaactag tttctgattt
    15181 taaaaataat aatatgccat tggtatcagc aaatatatat gaacaatctg gagaaaatta
    15241 tgtacaacca tatgtaatga aaaatattaa gacagatgag ggcaatgtaa aagttggaat
    15301 actaggtctt actataaaag aagtagggga aacccttggt cttgaggatt ttgaaaagga
    15361 taaaaaagct agagagcttg gagaacaagc tgattacaaa ggaaaattat atgcaaatga
    15421 tttagtagaa gatgcaaaga aatggataaa agttatggaa aaagaaagtc cagacataat
    15481 agttgcagtt gtacactcag gggaagaacc taaaacagat agaaatccag gaaatagaat
    15541 aaaagagtta gcaactacag tggatggaat agatgcaatt gtagctggtc atactcatga
    15601 aaaaatagat gaacacaaat ataaaaataa atctggagat gaagtaattg ttacacaacc
    15661 aggttggcat ggagatagag tatcagagat taatttcaaa ttaaataaag agaatggaaa
    15721 gtgggacata aaggaaaagt ctagtaaatt aaaaataatt gataaagaag taagtatagt
    15781 tgctactgca gatttacatt cacatttctc tgataaatta acagttgacc ttgttaatga
    15841 acgtagtaac ccaattgaac ctgttgtagt agatgcaggc gacttcttag accctcaaac
    15901 agatgaaatg actcaatggt ataaagagtg gaaaagtata agagataata atagtgataa
    15961 aacgattagt agatgcccaa tggtcatgga tatgaatcag ggaatgtatg atgcagtagt
    16021 acttggaaat catgaatttg tatccgaaaa agatgaattt tggtcagatg aacgcctttt
    16081 agatgctgtt atagaggatt ttgaacaggt ggcaatttct gtgttatcgg caaatatata
    16141 taggcaatct ggtaaaaatt atgttaaacc atatattata aaagatgtgg agacgagtga
    16201 aggtaatgta aaagtaggaa tattgggact tactataaaa gaagtaggta aaggtcttaa
    16261 aaatgtaaac ttacaagagc aatttcaata taaggataaa ttgtatgcaa atgatttagt
    16321 ggaagatgca aagaaatggg taaaagttat gaaaaagaaa aaaccagata taatagttgc
    16381 agttgtacat tcaggagaag aaccaaaaaa tccaaaacat ccaggaaata gaataaaaga
    16441 actagcaact acagtagatg gaatagatgc aattgttgct agtcatactc atgaaaaaat
    16501 agatgagcat gaatataaaa ataagtctgg agaaaaagtt atagtgacac aaccaggtga
    16561 gcatggtaaa tattattcta aaattacctt taaagtaaat aaagaaaagg gttcgtggat
    16621 tattaatgat aaattcagta aaacaattga ggttgaataa ctttataaaa tttatagata
    16681 aagtaaatta tctgatattg acatgatata taagataaag tttctaatat tagtataatg
    16741 cataatgtga aatattttaa taataagaga ataaacaaga caaataaaaa tattttaaca
    16801 tgtaagatat agtatgaact attatactaa tgcttacttt ataatttgaa catgttagaa
    16861 taagtcttat ttgaaaattt tagtgtatta gagtaagtat tattttacca gtaaaaaata
    16921 ttgagaacta tatgtagatt tgcttaaaaa ggaatttaca tatagttctt ttcgtataat
    16981 aaatagtaca atcttatatg gtaaagataa agtgtgatat taaattttat tatcatccaa
    17041 aatctaagga ggagtctctt atgaaaataa aatttattat agtagcatca ataatatttt
    17101 taatagtatt acttgtgggg tgtcaaagta ctaaagacac agaaatcaat gtactcgcca
    17161 caagtgattt acattcaatt attccagata atttaatttc ctatgtagaa gaggagagaa
    17221 aatatgatga gaatctactt atggtagatg caggagattt ttttgacatg caaagtggtg
    17281 agatgaataa gtggtttact ggtcaaaaat tagtaagatt taaagatggg ataccagagt
    17341 acaaaaaaat agctgaacca cgtgaaggag aagttccatt ggctaaaaaa atggcaaagc
    17401 tgaagtatga tgcagttaca tttgggaatc atgaatttgt atctaatgac aagcagagcc
    17461 ttgataaatt ggtttctgat tttaaaaata acaatatacc attagtatca gcaaacgtat
    17521 atgaacaatc tggagaaaac tacgtaaagc catatgtaat gaaaaatata aatactgaca
    17581 agggaactgt aaaggttggt atacttggtc tgactataaa agaagtaggg gaacgttcaa
    17641 ggtttaaaag ttttgaacaa gataaaaagg ctagagaact tggggaacaa cctcaataca
    17701 aagggaaatt atatgcaaat gatttagtag aagatgcaaa taagtgggta aaggttatgg
    17761 aaaaaaaaga aaagccagat ataatagttg ccattgtaca ctcaggtgaa gagccaaaaa
    17821 atcctaaaaa tccaggaaat agaataaaag aactagcaac aactgtagag ggaatagatg
    17881 ctattgtagc aggtcataca catgaaaaaa tagaacagca tacatataaa aataattctg
    17941 gtgaagaagt aatagtaaca cagcctggag ctcatggtaa tagtatatca aagattaatt
    18001 ttaagttgga taagaagaat gataagtggt ttataaaaga caaatatagt gaattaaaga
    18061 tttttggaaa ggaagtaaat ataattacaa cttctagtct gaatccaaaa ttttcagatg
    18121 aaatggcagt taatttgttt aatgaaagag atagagatgt tcaacctata tttatagata
    18181 ctggagattt tttaaatact aatacaaaag aaatgactaa gtgggctaaa gagtggcaat
    18241 acaatgaaaa taaaggcatc tataaaaaaa taaatgaatg gccaattgta tatcttggtt
    18301 atgatgctgt agtacttggt agccatgaat ttacagagga taaagagact tttgaggaaa
    18361 aaaaatctac attggattat attatagaaa actttgaaga aatgaagata ccagtattat
    18421 cagctaatat atatgatgaa tctggagaaa attatgtaaa accttatata attcataatg
    18481 tagaaactag ggaaggaaat ataaaggttg gagtattagg tcttactact actgataagg
    18541 atgaagaaga cttaagtagc tcaagcttaa gcaaagactc aaagacttcg aaaattcctg
    18601 aagaaacagg aaagttatat actaataatc tagtaaaaga tgcaagcgaa tgggtaaaag
    18661 ttatgcaaaa ggaaagtcct gatgtgattg ttgcagttgt acattcagat aataatagta
    18721 ctctaaaaaa tccaagtagt gaagtcaaaa aacttgcaat gtcagtagat ggaatagatg
    18781 ctattgttgt aggtcatacg aaaaataagg tagaagaaca tatttacaaa aataagtctg
    18841 gggatgaggt tatagtaaca caatctggag aagattgtta ttcaaaaata agtcttgaac
    18901 taagaaaaga aaatggcaag tggaatatca taaacaaata tagtaaagca ataaaaatga
    18961 agtgaattta ttgatatata aaccattatt aacactgatt tttataatat aataaatact
    19021 gattctataa tataatta
//
LOCUS       DAMKMI010000073        18862 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMKMI010000073 DAMKMI010000000
VERSION     DAMKMI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18862
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.074"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(305..697)
                     /locus_tag="PC429_002277"
     CDS             complement(305..697)
                     /locus_tag="PC429_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3847778.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1264..1626
                     /locus_tag="PC429_002278"
     CDS             1264..1626
                     /locus_tag="PC429_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847779.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1610..2317
                     /locus_tag="PC429_002279"
     CDS             1610..2317
                     /locus_tag="PC429_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847780.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            2337..2756
                     /locus_tag="PC429_002280"
     CDS             2337..2756
                     /locus_tag="PC429_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847781.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2746..3270
                     /locus_tag="PC429_002281"
     CDS             2746..3270
                     /locus_tag="PC429_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847782.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKCSQKYE
                     TDFKIKVWNFSESI"
     gene            3582..4139
                     /gene="efp"
                     /locus_tag="PC429_002282"
     CDS             3582..4139
                     /gene="efp"
                     /locus_tag="PC429_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF3847783.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4231..4686
                     /locus_tag="PC429_002283"
     CDS             4231..4686
                     /locus_tag="PC429_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3847784.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4849..5559
                     /locus_tag="PC429_002284"
     CDS             4849..5559
                     /locus_tag="PC429_002284"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF3847785.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5613..6683
                     /locus_tag="PC429_002285"
     CDS             5613..6683
                     /locus_tag="PC429_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3847786.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6711..10265
                     /gene="smc"
                     /locus_tag="PC429_002286"
     CDS             6711..10265
                     /gene="smc"
                     /locus_tag="PC429_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF3847787.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEIKDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEGSKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSINLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDENLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10292..11572
                     /gene="ftsY"
                     /locus_tag="PC429_002287"
     CDS             10292..11572
                     /gene="ftsY"
                     /locus_tag="PC429_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF3847788.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11675..12043
                     /locus_tag="PC429_002288"
     CDS             11675..12043
                     /locus_tag="PC429_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF3847789.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12040..13392
                     /gene="ffh"
                     /locus_tag="PC429_002289"
     CDS             12040..13392
                     /gene="ffh"
                     /locus_tag="PC429_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF3847790.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13430..13702
                     /gene="rpsP"
                     /locus_tag="PC429_002290"
     CDS             13430..13702
                     /gene="rpsP"
                     /locus_tag="PC429_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF3847791.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13767..13994
                     /locus_tag="PC429_002291"
     CDS             13767..13994
                     /locus_tag="PC429_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF3847792.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14102..14617
                     /gene="rimM"
                     /locus_tag="PC429_002292"
     CDS             14102..14617
                     /gene="rimM"
                     /locus_tag="PC429_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF3847793.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14626..15321
                     /gene="trmD"
                     /locus_tag="PC429_002293"
     CDS             14626..15321
                     /gene="trmD"
                     /locus_tag="PC429_002293"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF3847794.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15506..15856
                     /gene="rplS"
                     /locus_tag="PC429_002294"
     CDS             15506..15856
                     /gene="rplS"
                     /locus_tag="PC429_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF3847795.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16177..17073
                     /gene="ylqF"
                     /locus_tag="PC429_002295"
     CDS             16177..17073
                     /gene="ylqF"
                     /locus_tag="PC429_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF3847796.1"
                     /translation="MSNEYEEYLMNDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17462..18754
                     /gene="brnQ"
                     /locus_tag="PC429_002296"
     CDS             17462..18754
                     /gene="brnQ"
                     /locus_tag="PC429_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF3847797.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7974 a         1761 c         3467 g         5660 t
ORIGIN      
        1 tacacattaa tatctataaa atgacaaaat aaaaaatact atttaaatag atattaaaaa
       61 attgatacca agtatttgtt tgctatatga ataacaattt atttaggagc tttacttgga
      121 cactgtataa taataaagta attgctaaaa acgtttgatg atttggatgt atttttagaa
      181 attagaaaaa tatatactta ctgactaatg ataatcttat atgtatttta gtttgagtga
      241 tttaagaaaa gtgttttgaa tggggattat tttatagagt aaataaatat ataatagtag
      301 aaaattacat ataaagagta gtatttattt cattaccttc tttatctgtt ccgatagggt
      361 cttgtagaga tacttgagtt ttattttttt tgttagatct aatattcatt aaaatctcat
      421 tttcaataca tctagaagca tatgttgcta atcgagttcc tttatctatg ttataagttt
      481 ctatagcttt aattaatcct attgtaccta tggatattaa atcatcctga tcttccgttg
      541 aattattata tttctttgct atataagcta ctaatctcat gtttctttca attaatgtat
      601 cttttgcact tttatcgttt tctattttaa atttagttaa atactcaatc tcttcttcag
      661 gggttaaggg tttttcaaaa gatttaagag ctgccaatag gtcaccccct tttcaacggt
      721 cactttaaat tatatgaagg agacttatcc aagttgactg ttatctaata aaataatatg
      781 catattttta gaatttaaaa catatatttg caaacttttg aatggaatat gacataaaga
      841 gtgggaattt aaatttaagt ttttgttgta tatatatgaa acaaggcgaa gaaattattg
      901 atttataaag tataaattgg aattgaggtg tcaataaata taaaaggaaa tagtatttat
      961 atatgttttg gtgttgaagt aaaagattaa tgcaaagcat tagagtctat aaaaatatga
     1021 atataggagg tgtaatttta caatacttaa aaaagtaaat gtaattaatt gtttgtataa
     1081 agttgattac aagcaaaagt atcagtcata gttaatactg ttaaggatga aggtggtgtg
     1141 ttaatttata tatgtatggg aagaaatttt gattacaatg aaaaaactta caaaagatat
     1201 ttacgaaaaa acttatagtt aaatgtttat atataaaata ttatttaggg gttgttttta
     1261 ttattgaaaa taagaaagag tggttttata tcaattgaat gtataataag tattgctata
     1321 ttatatgtgg ctgtttattt agtttctaca tcattgtata attgttatag ttttatcagt
     1381 agaaatatat ctgacagaga aatgttaagt acagcaaaaa aatatataga agatgagaag
     1441 tatagaatac aaaatagtaa gtatgagtta attgaagata agatagaaaa aaattacata
     1501 aatggatatg aaattaacag tagaatagag caaattttag attattatca atgctatgaa
     1561 ataaatatag agataaaaaa tgaatttaaa aaactgaggt ttaatagcta tgttactaga
     1621 aaataaaggc tctatattaa taataacatt gataatattt tctattataa gtactgtgtg
     1681 cattatgtgt ataggtttaa tttatacaca taataatatg tttgagttag gatacaaaga
     1741 cattcaacta aaagaaattt ctttaagtgg aatagaaata tcagtaagta atgtactttc
     1801 ttgtgttgag gaagctattg atatatctaa taatgaagac gaatttagag aatatttctt
     1861 aggaaataat atgattgaat gtataaaaaa aataaaagat acatcttata gtagtttaga
     1921 agaactttct ttaaaagtag aaaataatac tatttatgac agaggagatt tttttgaatt
     1981 tgatatagaa agtaatgtta gagaaaaaga atttttaaaa gcaataaaag taagagtgaa
     2041 aatcaaaaat ccatggatag gaattgatat tgaagattat atggataaat ctaataacaa
     2101 taaaattgat gacaaaacat tcaataatta taaagcatct gaaaataatg aaattataaa
     2161 tcaatttaaa aatgaaagtg aaaatataag gaatcaagag gttgttgaat ttacagaaga
     2221 agatgaagaa gaacttaaac aaaacataat tgaaaatata aagaagaatt ttgatgaaaa
     2281 aaacctagta caggtatata agtatagggg tgtataactt tgaaaaaaga aggaatgtga
     2341 ctgtaataga attaatagta tctatcagta taatactctt gatagttaca ttatcttttc
     2401 caaaagataa tcttgaaaat cataaaataa atttatttgc acgtcaactt tgttcagata
     2461 taaggtatgt taggcgaatt aatatgttta gcaactacaa tacatatata tattatataa
     2521 atcaaggtaa acacgaaggt tatgtattga gaaaagatgg taaaaacatt aaatcaataa
     2581 tgctacctaa aaatgcaaaa atagctagga gtattgaaat aataaagttt aaatctgatg
     2641 ggtcgcctca aaaggcgtct acaatagagg tatataataa tagattaaga aaaactataa
     2701 caataacacc tgtaagtgga agagtacttc tgaaagaggg aaaatatgaa acataaatat
     2761 ggttacttgc ttttggaaag tgtcgtatca ttgtcttcaa tggtgataat tatactagtt
     2821 ttatattcta tatttttgtc tacaataaac ttaaagttaa aagtagaaga taagatagaa
     2881 ctacaacaac aaagtcttga aatcataaaa tctatggaag gaattattag caattctatg
     2941 ggaataatga atgtatctaa ttatgaagaa acatttaaaa agacaacatc tataaaatgt
     3001 agatatttag atgaaaataa taatgaagaa ggcataagta ataaagaaat tatattgaat
     3061 gaaagacgaa ataagttatt tgtaaattct ttaaatggcg aaagtagtca agcaggtggt
     3121 tatgaaattg gagattatgt agatgaaatg tatgtatcaa ttactaataa tggtcaatac
     3181 gttaatatta aattgaaatt atcaaaatgt agtcaaaaat atgagactga tttcaaaata
     3241 aaagtatgga attttagtga aagtatataa aaatacataa atttaagaga aagaaatgac
     3301 atcaataagt catataaaat ataattattg agaactatat aactaaataa tatcaatatg
     3361 taaagtgtat agattaagaa tatagaggat aataataaaa atatagaata agtaatttgc
     3421 caaatacttg tttatattgt ataacttatg atattatgat attagtaaaa atttgtaatc
     3481 ctgacttgac agaattcaag taagagatat aattttaaag gcatagtaat gtaaaattta
     3541 ctaaaaaata agaaatttat tttaatatgg aggaacaaaa aatggtatca gcaggtgatt
     3601 ttagaaaagg tgttacattt gaaaaagatg gacaaccatg tttagtagtt gattttcaac
     3661 acgttaagcc aggtaaagga gctgctttcg ttagaactaa atacaaaaac ttaaaaacag
     3721 gagctataag agaagaaagt ttcaatccaa gtgaaaaatt ccctaaagct gttatagata
     3781 caagacaaat gcaatatcta tacaatgatg gtgagttata ttattttatg gatcaagaaa
     3841 attttgaaca aataccatta aactatgagc aagttgaaga tgcaattaag ttcttaaaag
     3901 aaaatgaagt tgctacaata agattttacc aaggacaacc attccaagtg gaagcgccaa
     3961 actttgcaga gctagaagtt acagatacag agccaggcat aaaaggtgat acagcaagta
     4021 atgtaactaa agcagctaca gttgaaactg gagcagttgt tcaagttcca ttatttataa
     4081 atactggaga taaagtaaag atagatacta gaacaggtga atatttatca agagtataaa
     4141 aatttatgta tataaaagct tatatgtctt aataatataa gatatatgag cttttagttt
     4201 atatataaaa aagaaagcga ggggtgttac atgaaacatt tatatgaaga agttgcatac
     4261 ttaaaaggcc tagcagaagg actagaaata agtgctgaaa gtaaagaagg gaaaatgata
     4321 cataaaatag ttgatgcatt agaagtattt gcagatgcta ttgtaacatt ggatgaagaa
     4381 caagaagaac ttcaagattt tgttgaatct atagatgaag atttagcaga tttagaagaa
     4441 gatattgctg acatggaaga agatctttat gaagaagatg acgatgagga tgatgaggac
     4501 ttcagctata tagaaatgga atgcccaaat tgtggagagt tagtagaaat agacgaagat
     4561 ttgttatatg atgatgaagt agatgttgtt tgtcctgatt gtaaggctgt gatattatca
     4621 tcagaagatg attgtgatga tgattgtaca tgtggtggtt gttcaagttg tgatgataga
     4681 gagtaaaaat tcataagaat taaaactgcc tatgatgcct atgagatatt ttgcttcata
     4741 ggtagtttta tttttatgag gaagggtatt atgtattttg gttttgcaat acttggacaa
     4801 aaatagtaca ctagaattta tagaatagga ttttggaggg atgtaaatat gaaaataagt
     4861 aaaaaattac tagataatat acaaagattt gaaaatgtta taaattataa atttaaaaat
     4921 aaagaatata tactagaagc tcttactcat agttcatatt ctaatgaaaa taaaaaatat
     4981 aattttaacg aaagattgga atttttaggg gattcggttt taggcatagt gataagtgat
     5041 tatttattta atgaagaagc taatctgccc gaaggggaac taactaaact tagagcaaat
     5101 atagtctgtg aagattcatt aagtgaagta gctaatgata taaatttagg tattcacatg
     5161 ttattaggta gaggagaaga agcaacagga ggaagacata ggacttcaat attagcagac
     5221 gcatttgaag ctgtaatagc tgctatatat cttgatggag gttttgaaag tgcaagacag
     5281 tttatacttc atcatatgga aaatataatt tatgattcaa gaaaaggaaa tatatttaga
     5341 gactacaaaa cgcatcttca agaggtgtta caagggaatg gagaaaataa tatctggtac
     5401 aggcttatag aagaaaaagg accagaccat aataaaagat ttgtaatgga agttggaatt
     5461 aatgacgatg tacttggtat aggagaaggc aagagtaaaa aagaagctga acagttagct
     5521 gctaaaatag ctttaaagaa aaaattatgg gaaaaataaa tataatatgt aaaaatataa
     5581 aaagaaatat ttatattaga aggatgttgt agatgaaaaa gagaattata cctatatttg
     5641 ttcctcacaa aggatgtcct catgattgca tattttgtaa tcaaaaaaaa ataacaggtg
     5701 tgagtactga tgttactagt gaagatgcaa gaaatataat tgaggaatgt ttagagacta
     5761 tagataaaga tgcagatgta gaaatagcat tttttggtgg tagctttact gctatagata
     5821 ttgatataca aaaaagttta ttatcagttg ctaaagaata tgtagaaaaa ggtcttgtaa
     5881 aagatattag aatgtctaca agaccagatt gtattgatga agatatatta agtatgttaa
     5941 aagaatataa gacaagtata attgaactag gagtacaatc cttagatgaa aaagtattat
     6001 tagatagtat aagaggtcat caatctgaaa ttgtatacaa gagttcaaag atgataaaaa
     6061 atagtggaat caaacttgga ttacagatga tgattggtct tccagctgac acagaagaaa
     6121 agtgtatatt tacagcaaaa aaatttgtag agctaaaacc agattgtgtt agagtctatc
     6181 caactttggt agtaaaagat acaggtcttg aaaagttaat ggaacaaaat aaatataatc
     6241 catttaccct tgaagagagt gtaaatatag taaaaaaagt tcttgtactg ttttatgtta
     6301 ataatatcaa tgtaataaga gttgggcttc aagctactga tgatatacaa attggaaaag
     6361 ctgttttagc aggaccatat catcctgcat ttagagagtt ggtagaagca gatatgataa
     6421 aagattattt agaatttgtt attttgcaaa ataaaaatat aaagcaaatg ttggtcaaat
     6481 ctaataaaaa gaatatttct aaaatcatag gaaataaaaa aacaaatgtt aaatatatgg
     6541 aagaaaaatt tggtgtgctt ttaaaaacac aagaatcaga tttagatata aatcaattag
     6601 aaattgtgtt agatgggaaa agtttaatta gtgctaatat gagagacatt catagaaaat
     6661 tgtatgacat ttatgacctt tagttttaat aatgaaggga gagaaaagat ttgtatttaa
     6721 aaagactaga gttaaaggga tttaaatcct ttccagtaaa aacagatatt atttttaaag
     6781 aaggaataac agccatagta ggaccaaatg ggagtggaaa aagtaatata tctgatgctg
     6841 taagatgggt attaggagaa cagagtatta agagtcttag aggtgataaa ctcgaagatg
     6901 taatatttgc aggtacagat acaaaaaaac ctatgaatta ttgtgaagtg gctcttacta
     6961 ttgataattc tgaaaatcaa ttggaattag attttacaga agtaactata agaagaaggg
     7021 cttatagaaa tggtgaaagt gaattttttc taaataataa aagttgtaga ttaaaagata
     7081 taaaagaagt ctttttagat actggtattg gaaaagatgg ttattcaatc atagaacaag
     7141 gaaaagtaga tgaaatatta agtaataatc ctttaagcag aagaaaagtt tttgatgaag
     7201 cttgtggcat atcaaaatat agatataaaa aacaggaagc agaaaggaat ttaagtaata
     7261 caaaggaaaa tttagaaaga atagatgatg tatatataga aattgaaaat caattaaagc
     7321 ctctttttaa tcaacagaca aaggcaaaaa agtacttgga aataagtgaa aaattaaaga
     7381 cacttgaagt aaatagcttt ataagagaaa ttgaaggaat agaaaaagaa ttaagtgagg
     7441 ttaatgaaca tcgtaaggtt attgaaaagg agctcaatga aaaagaagaa caaaaaaatg
     7501 ttgtagagaa aaagcaagaa gatattaata aagaggtaga ggtattacaa gatgtaattg
     7561 aaaaatctgt agattatata aattcaatta aaggagttat atcaaaaaaa gaatcccaaa
     7621 taaatttaat taaggaaaga ataagaaatt ttacgaatga aattagtaga aaaaatttag
     7681 aaataaagga tataaaagag aagctaaatg aaaataagca atacataaaa gaattagaaa
     7741 gcaataaatt atcaggtagt gaagaactat ctaccttgca ggaaaatata aaagtattgg
     7801 aaggaagtaa agataaacaa aaaattaagt tagaatccct aaataatgag atagaattat
     7861 tgaaagaaag tataattgat atattaaata agaaacaaga gtttagtaat aagctgtcta
     7921 ctttaaacgc aaataaagaa aatatgaata tcagagatga aaatataaat tcagagataa
     7981 cagagttaaa caaaaatatt gaaataaaat catctgaatt agatactata aataaagaat
     8041 ttaatatgca aaatgaaaat ttaaagaatg taaataatag acataaagaa ttatcaatta
     8101 atttgcagga ttcaattagt gaacacaata aactagaaga tgaaatacag aaaagtaaat
     8161 ataatttaaa tggatataat tcaaagttaa atgtctatat agatatggaa aatcattatg
     8221 aaggatttaa tagaggagta aaagaagtac taaaaaataa aaatttaaaa ggagttcatg
     8281 gagcattagg acagattata aatgtacctg aaaagtatga gaaatctata gaagcagctc
     8341 ttggtgctta tatgcaaaat ataataactg ataatgagtt tagtgctaag tctgcaataa
     8401 actatctaaa acagaataat ttaggtagag taacatttct tccattaaat ataattaaat
     8461 caaataaaat aagtctagga aatttaaaag caaatactaa gttcatagga atagcaagtg
     8521 atttaattac atttgatgaa aagtatagaa atataattga aaatatactt ggaagaacaa
     8581 tacttataaa taatatagat gagggcataa aatttgcaaa agaaactggt cataggttta
     8641 aaatagtaac tttagatggt gaaatcttaa atccaggagg ttctttgact gggggaagtc
     8701 taaaaactaa tgggaatata ctatcgagaa aaagatacat taatgaatat acagaaaaga
     8761 taagtaatat caagaatgaa atttcacatc tagaattaaa aagagaatct ttagacaaag
     8821 atgtgaaaaa tatcaaaaat gaaattgatt cacatgaaag taaaataaaa gatttagaaa
     8881 aaagtataat aataaaaagt acaagtataa agaatgttga gtctgaaatt gaatcgttaa
     8941 aaggtagtat tactaaatta gaaaatgaga agaatgattt aaattctaac ctaaattata
     9001 cacttgaaaa aagtgatgat gttagaaaag atatggaaga gttagatgat ttatacaata
     9061 aaaataaaga aaaaattgat gcattgaatg aggaaataaa aagatacaat gatttatatg
     9121 ataaagaaaa atctgaattt gatgaattga acttaagttt agtgaagaaa acagaagttt
     9181 acaatagtat agttagagat ataaaaagaa tatctggtga aaattgtgag ctagaagaaa
     9241 aaaataaaca attagaagag tcattaaatt atgaagaaca tgaaataatt aaattacaag
     9301 attctatact cactgaagaa aaagaaaaag aaaatttaac aaaacaacta ggagatagta
     9361 atagaaatct tgaaactaga aaaatagcaa aagatgattt aaaaaatagt tttgatgaaa
     9421 ttaataaaga attaaaaact atagatagac agcatataga acttaaagaa agtttattta
     9481 aagttggtgg aagattagaa agattaaaaa ctagtcaaga cacatatata aataagttgt
     9541 ttgagcaata tgatatgacc ttagtacaag cattggaaat taaagatgaa aatttggata
     9601 tagatagaaa attcttagaa agtctaaaga gagaaataag aagtcttgga aatataaata
     9661 tagactctat aaaggaatat gaggaaataa aagaaagata cgatttttat agcgaacaaa
     9721 agcaagattt agaagagtct atggaagaga tagaaaaact tatacatact ttagaagaaa
     9781 atatgaaatc tgaatttgaa attaagtttg aagaaataag taaaaatttt aaatatgtat
     9841 ataagagatt atttggtgga ggttgtggag aattaactat tttagataaa gaaaatttat
     9901 tagaaagtga tatactaata acagctcaac ccccaggtaa aaaaatgaaa aatttaaacc
     9961 tattatctgg tggagagaaa gctttaacgg caataagtat attatttgct atattgatta
    10021 caaagccaac accattttgt atattagatg agattgaagc gccacttgat gacgcaaata
    10081 tatttagatt tggagaattt ttgaaggatt tatctaaaga gactcaattt atatcagtaa
    10141 ctcataggag aggaactatg gaagctgctg attatatcta tggagttaca atgcaagaaa
    10201 aagccatttc aaaagttatt agtttaaaat taaaagaagc tcaggaaata acagatatta
    10261 tataacttag attgacatag gaggaataac agtgttaaaa aaattatttg gttttggaaa
    10321 agacaaggaa aaggaaatag aaaaaaaaga tgctgaagaa gaaatagaag ttgaagacag
    10381 tgttgataat ttagaaaact tggaagaaac tattttttca ggacttgaag aagaagtgat
    10441 agataaagtg gaagatgttg aagaaaaaaa tgaggaaagt gtcaataatg aaggcactga
    10501 agaaatcgaa aactttgaaa aaatagaagt tgagatggat agtaatggaa aagaagtaga
    10561 aaatatttca aataatgatt ctcaagaaga gataatcgaa gaattagaaa attatgatga
    10621 aattgagtct aaagagatag aagataaaga agataagaaa gttaacttgt ttgaaagact
    10681 aaaacaagga ttgacaaaag caaagcaagg aataactgat agaatagatg aagttttaaa
    10741 atcatataca aagatagatg aagaattact tgaagattta gaggaaattt taataacagc
    10801 agatgttggt gtaaatacaa ctatggatat aattgaaaga ctaagagata aaataaagca
    10861 aaaaggtatt actgaaccaa taaaggtaag agaagaactt aaatctatag ttgaagatat
    10921 tcttacaaat gaaaactcta cactagatat agaaccagca ccatgtataa tattaatggt
    10981 gggtgtaaat ggggtcggta agactactac aataggtaaa ctagcaaata gatacaaaaa
    11041 agatggtaaa aaagttttat tagcagcagc agatacattt agagcagcag cgacagagca
    11101 attagaaatt tgggcaaata gaaccaatgt agacataata aaacatcaag agggagcaga
    11161 ccctggagct gtagtatttg atgctataaa agctgcaaaa gctagaaaaa cagatgtatt
    11221 aatatgtgat acagcaggaa gattacacaa taaggctaat cttatgaatg aacttggaaa
    11281 agtatttaaa atagtagata gagaatttcc agaagcaaaa cgtgaagtac ttcttgtggt
    11341 ggatgctaca acaggtcaaa atgcagttgt tcaagcaaag acatttaaag aagttgcaga
    11401 tataacaggt atagtactta caaaacttga tggaactgca aaaggtggag tagtccttgc
    11461 agtaaaatca gaagtagatg tacctgtaaa acttattgga gttggagaaa gtgtagaaga
    11521 cctacaagat ttcaatgcaa aatcattttc agatgcatta tttggtaact aatgttcttt
    11581 taaaaataat ggttgacaag agtgttgtta tgatataaaa tacaatagtg taaagtaaat
    11641 aacctttaca gtatactgct aggaagtgta agatatgaat atagaaaaaa tggtcgaaat
    11701 tggattgtta tttgaacagt ataaagaatt attaactgac aagcagaaag aaatagttgc
    11761 tctatactat gaagaagatt attctttagg tgaaattagt gaaaatctga atgtatcaag
    11821 acaaggtgtt tatgatacct taaagcgttc agaaaaaata ctaagagatt atgaagaaaa
    11881 gttacactta gtatcaaaga ttcaagagca ggaaaaaaat attaagatta taaaagataa
    11941 aattattgac attaaagaag atttattgca caatagagat tgtgctaatt taatccctaa
    12001 gctagaaaat atagaagatg tatgtaggga gatgataaaa tgatatttga aggattgtct
    12061 gataaactac aaggggcatt tagtaagtta aaatctaaag gaaaacttac agaagtagat
    12121 gtgaaaaatg ctatgagaga agttaaactt gctcttcttg aggcagatgt taactttaaa
    12181 gtagttaaag atttcataaa aaaagttcaa gagagatgtg ttgggcaaga agttatgcaa
    12241 agcttaactc cagctcagca tgtaatcaaa attgtaaatg aagagcttac aagtttaatg
    12301 ggagatgtac aaagtaaagt aatgatttcc tcaaagcctc ctacaatttt aatgatggtt
    12361 ggattgcaag gtgcaggtaa gactactaca gcaggaaaac ttggaggata ttttaaaaag
    12421 caaggtaaaa aacctctatt agtagcttgt gatatttata gaccagcagc tataaagcaa
    12481 cttcaagttg ttggagaaaa attggatata cctgtattca atatgggaga taaagaaaat
    12541 ccagtaaata ttgcgaaagc aggtttaagc catgcgatta aaaatgctaa tgatttagtt
    12601 attatagata cagctggtag acttcatata gatgaagtac ttatggatga gttaaagtct
    12661 attaaatcag aagttaagcc acatgagata cttttggttg tagattctat gacaggtcaa
    12721 gatgctgtaa atgttgcaga aagcttcaat gaggcacttg gtgttgatgg agtagtatta
    12781 actaaattgg atggagatac tagaggtggt gctgcacttt caataagagc agttacacaa
    12841 aagcctataa aatttatggg gatgggagaa aagctagatg atatagaacc tttccaccca
    12901 gatagaatgg catcaagaat attaggtatg ggagatgtct taagtttaat agaaaaagca
    12961 caagaaaata tagacttaga gaaagctaaa gaattagagc aaaaaattaa aaaacaagag
    13021 cttgattttg aagatttctt acaacaaatg gaacaaatac aaaatatggg acctcttgac
    13081 aagatacttg gtatgatacc tggtatggga aatgtaaaag accaacttgg agatgtagat
    13141 ttgaatggca aagaaatgaa gagaacaaaa gccataattc agtcaatgac tacatatgag
    13201 agaagaaacc caggagtatt aaatgcttca agaaagaaaa gaatagctaa aggcagtgga
    13261 actagtgtac aagatgtaaa tagacttata aaacaactta atgaaatgaa aaaaatgatg
    13321 aagatgtttg ctggttcaca aaagagtatg aagaaaaggg gcggcttagg cggtttacct
    13381 ttttttaaat aaaatataaa aaagtaaaaa ttattcggag gtgactgtaa tggcagttaa
    13441 aataagatta aaaagaatgg gagctaacaa aaaacctttc tacagaatag tagtagctga
    13501 ttcaagagct ccaagagatg gaaaatttat agaagaaata ggatactata atccaatttc
    13561 tgaacctaaa caagtaagaa taaatgatga aaaagctata aaatggttag ctactggtgc
    13621 tcagccaaca gaagtagtta aaaaattact tgtaaaaaac ggagtaatag aaaaattcga
    13681 agcttctaaa caagcaaaat aattttttct tggcaattag tttaagtaag ggctcattgc
    13741 taaggttact ttaggagatg aaatatatga aagagctagt tgtagatata gctaaggctc
    13801 tagttgataa tcctgactca gttgttgttg aggaatttga agataatgat ggtatcgtct
    13861 taaagcttac agttgctcaa gaggatatgg gtaaggttat tgggaagcag ggaagaatag
    13921 ctaaagctat aagaactgtc gtaagatctg ttgcaaatag agaaaatata aaagtttctc
    13981 ttgagatagt ataaaatagg aggattaggt ataatgccta gtccttttta tataaatgcg
    14041 tagtttaaga ttttatgatg atatgaccac ataagattaa attttttaag aaggtgataa
    14101 catgaaagaa aaattaactc attttaaggt aggtcaaata gtaaatacac aaggtttaaa
    14161 aggtgaagta agagtttatc cacttactga tgatatagag aggtttgatg aattaaaaga
    14221 tttctatctg ggaaaagatt taaataataa attggctgtt gaaaaagtaa gatacaaagg
    14281 ttctatggtt ataatgaaga taaaaggcat agacagcata gagaaagcag aaaaactaaa
    14341 aaataaattt atgtatgtat ctagagaaga tagcagagag cttgaagaag gcgaattttt
    14401 tatagcagat atgattggaa tggaagtttt aactgtagat ggaaaatatg taggaatact
    14461 agaagatgtt cttcaatact cagcaaatga tgtctatgta gtaaaaggtg aggaagataa
    14521 agaatttatg ataccagcta taaaaaaatt tgtacctaca atagatattg atgaaagaaa
    14581 aatgattata gaccctatta agggaatgat agattaggtg gtaatatgag atttcatata
    14641 atgacacttt ttccagaaat atttaattcg tacatggatg aaagtataat gaagagagca
    14701 gttgaaaaag gtataattga ggttcatata tataatataa gggatttttc taataataaa
    14761 cataaaaaag tagatgatta tccatttgga ggaggagcgg ggatggtaat gactcctcaa
    14821 cctatatatg acacatataa gcatataata acaactcata atataaataa cccaagtgta
    14881 atttatctta ctccaaaagg taaggtatac aatcaatcta tggcaaagca gatgtcttta
    14941 aaagaagata taatactact atgtggtcat tatgaaggaa tagatgagag aatcattgat
    15001 ttaatagtta ctgatgaaat ttcaattgga gattatgtat taactggggg agagctccca
    15061 gcacttataa tgatagattc tatatcgaga ttaataccag gtgtattaaa ccaagaagaa
    15121 tcatttgaag aagaatcatt taaagataac ttattggaat atcctcatta cactagacct
    15181 agagattttg aaggattaaa agtaccagaa gtgcttttat cagggaatca taagaagata
    15241 gatgagtgga gaagagaaga atctataagg ataaccaaag aaagacgatt tgatttatat
    15301 aaaaagtcta atgaaaaata aaaatataat gaaaaattaa tatatatgta tacaataaag
    15361 tacctataaa tggaattaaa aacataaaag tgcttgtaat aactattgaa gtatagtata
    15421 atataaaatg ttgaaaatac gggcgttcct ctgtttacac tggtcgggta gttaagaacg
    15481 tctatgatat ctaggaggaa gaaagatgaa tgaaatgcta agagcaattg agcaagaaca
    15541 attaaaaaat gaagttccta attttggacc tggggacaca gttaaagtac acgttagaat
    15601 tatagaagga aaaagagaaa gaatacaagt attcgaaggt gtagtattaa agagacaagg
    15661 tggaggagca agagaaactt tcacagttag aaaaatgtct ttcaatgttg gtgtagaaag
    15721 aactttccca gttcactctc caaaaataga aaaaatagaa gttactagaa aaggtaaagt
    15781 aagaagagct aaactaaact acttaagagg tagagtaggt aaagctgcta agataaaaga
    15841 agctagaaat aaataattaa aaactttact tataaatggg gggctgattt atcagtcctc
    15901 tttctatttt aatgaaaatt acagcaaata attatactag aaataaatag aaatatttgt
    15961 tgaaaatata cattatttat ctatgaattt atagctttta aaatgaaaaa taaaattggt
    16021 aaatattgca ttatattttg taacaatatt aaaaataaga gaacagtatt atgtaaagta
    16081 tgacgttaaa aagatgacaa tattatagtt tgttaagata gattttttta gtggaattag
    16141 gatataataa tataatatta ataaggagtg atacatgtgt caaatgaata cgaagagtat
    16201 cttatgaatg acaatctaca tataaactgg tatccaggtc atatgaaaaa aactaaggag
    16261 ttagtaaaaa ataacttaaa attgattgat gtagtaattg aacttttgga tgctagaatc
    16321 ccgtttagta gtaaaaatcc agacatagac aggcttgtag gagataagcc aagagttgtc
    16381 gtacttaata agagtgatat ggcagatagg gataaattga atcaatggat tgaatactat
    16441 aagaaaatta atatcaaagc tataccagta gatacaataa aaggcgttgg tataaataag
    16501 ataatcgaag aatgtaaaaa tgtaactaga gaaaaaatga gttctttaaa agacaaaggg
    16561 agaaaagaaa gagctatacg aataatgata gttggagttc caaacgttgg taaatcgtct
    16621 cttataaaca aattaacagg tagaaaaagc actcaaactg gagataagcc aggagtaacg
    16681 aaagggaaac aatgggttag attaaaggga aatcttgaac ttttagatac acctggaata
    16741 ctttggccta agtttgaaga ccaagaagtt gcattaaatt tagcatttag tagagctata
    16801 aaggatgaga ttttagatac tgaaactttg gctttaagac ttatagaaaa acttatgaaa
    16861 atagaacctg agaagttgaa agccagatat aagttagatt gtctaggaga aactccaatt
    16921 gaaactatgg atatgattgg ccataaaaga gggtttataa caggtaaaaa agaactagat
    16981 tatacacgca tagccacaac tgtattaaat gaatttagag atggaaaaat aggaaatata
    17041 acattggaag ttccagaaaa cgttaaaaga tagattttaa aacaaaatca aaaatttaat
    17101 gttaaaaatt taaaaaagcc ttttagcaaa ggctttttta tttttttgaa atatagacaa
    17161 atatctccat ataaaagtat aaattaggtt agaaaacctt taaaaatatt aatttaagac
    17221 aaatatacct aatattttga aaattaaaat gagtgtgatt atttaaaatt ttagcatatt
    17281 gacttgcttg tttcgtataa tatataataa gcacaaataa atacaatata ttaaaaattt
    17341 aatttattta attttattaa tttaataata agttcttatt gtttaattca acttacttat
    17401 gcaagcgtaa acaatcgaga cattttatta atttaataag gttaaaaagg gggattttat
    17461 aatgggtaag accaaggatg ttatagtatt tggatttgcg ttatttgcaa tgttctttgg
    17521 agcaggtaac ttaatatttc caccttactt gggaataatt acaggtccag aatggttaat
    17581 tgccttctta ggatttacgt ttgctgatgc tggattggca cttctagcag taatggctac
    17641 agctaaattc gatggaaacg tagttgaaat gtttaaaaga tgtggtatga agttagggat
    17701 tcttataggt tgtgcagata ttttatgtat aggaccattc ttagctatac caagaacagg
    17761 tgcaacaact tatgaaatgg gtataatgcc tttatttgga acttcaattc cagtgttatt
    17821 attctgtata cttttttttg cgatttcata tgtactaaca ataagacctt ctaaggtcgt
    17881 agatatagtt ggacagtttt taactccagc tcttctaata gctttagcat ttataattat
    17941 aaaaggtatt atatcacctc ttggagatat agtagataaa cctatgatac ctaatgtatt
    18001 tgcagaaggt ataggtcaag gatatcagac aatggatgct tttgcagcaa tagcacttgc
    18061 atcagtgcta atagtatctt taaatgataa aggatattct actattagtg acaagttgaa
    18121 aatgattggt aaggcgggtg ttcttgcttg tggaggatta gctctagttt atggaggatt
    18181 atgcttcctt ggggcaactg tatcaacaat gtatggaaca gatgcagtac aatcacaagt
    18241 aatagtaaat ataactgaag gattgcttgg aaatgttggg aaagcgattt tagcagtagt
    18301 agtatcatta gcatgtttaa ctacatctat aggtcttaca tctgccacag gtcaatattt
    18361 ctctagactt acaaaaggta aattgagtta tgaaaaaata gtattagctg tatctgtatt
    18421 cagtgcagta gtagcatctt ttggtgttgg aactataata aagatagcat ctcctatact
    18481 aagtatagtt tatccaccat caattgtttt aataatttta gctttcttca atgaaaaaat
    18541 taagaatgat aatgtataca aaggagcagt atatatgtca ttgcttgtaa gtatactaac
    18601 agttatatct agttatggag tagctgttcc agtagtaaat tctttacctc ttaattcatt
    18661 aggatttaac tgggtggtgc cagttataat agctggaata attggtaatt ttataccatc
    18721 aaagagtcaa tctaatacac ttggaacaaa ctaagttttg tttaattaaa atataatcaa
    18781 ttgtaaataa atgagttaac taaaagtaaa aggagttatt tgtacaaata actcctttta
    18841 tttttagatt ctatatttaa tt
//
LOCUS       DAMKMI010000074        18598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMKMI010000074 DAMKMI010000000
VERSION     DAMKMI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.075"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..249
                     /locus_tag="PC429_002297"
     CDS             <1..249
                     /locus_tag="PC429_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF3847798.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIRTIG
                     FGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSVYKK"
     gene            265..1848
                     /locus_tag="PC429_002298"
     CDS             265..1848
                     /locus_tag="PC429_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF3847799.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1876..3204
                     /gene="ssnA"
                     /locus_tag="PC429_002299"
     CDS             1876..3204
                     /gene="ssnA"
                     /locus_tag="PC429_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF3847800.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3349..4701
                     /locus_tag="PC429_002300"
     CDS             3349..4701
                     /locus_tag="PC429_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF3847801.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4893..6002
                     /locus_tag="PC429_002301"
     CDS             4893..6002
                     /locus_tag="PC429_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3847802.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6285..7676
                     /gene="hydA"
                     /locus_tag="PC429_002302"
     CDS             6285..7676
                     /gene="hydA"
                     /locus_tag="PC429_002302"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF3847803.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7670..10231
                     /gene="xdh"
                     /locus_tag="PC429_002303"
     CDS             7670..10231
                     /gene="xdh"
                     /locus_tag="PC429_002303"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF3847804.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYIFDKEFRTELPLQNTPYSKKK"
     gene            10383..11729
                     /gene="rpoN"
                     /locus_tag="PC429_002304"
     CDS             10383..11729
                     /gene="rpoN"
                     /locus_tag="PC429_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF3847805.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
     gene            12527..13549
                     /locus_tag="PC429_002305"
     CDS             12527..13549
                     /locus_tag="PC429_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3847806.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            13609..14616
                     /gene="gap"
                     /locus_tag="PC429_002306"
     CDS             13609..14616
                     /gene="gap"
                     /locus_tag="PC429_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF3847807.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            14838..16040
                     /locus_tag="PC429_002307"
     CDS             14838..16040
                     /locus_tag="PC429_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF3847808.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            16066..16809
                     /locus_tag="PC429_002308"
     CDS             16066..16809
                     /locus_tag="PC429_002308"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF3847809.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            16818..18350
                     /locus_tag="PC429_002309"
     CDS             16818..18350
                     /locus_tag="PC429_002309"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF3847810.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         7460 a         2141 c         3747 g         5250 t
ORIGIN      
        1 gaagaagatg aatttgttca agcagcgtat aagggattaa aagaagctgg aatagattca
       61 gaaataactc aatactcatt ctgtacgaat ggaagtcact acgcaggtga ggctggaata
      121 agaactatag gatttggtcc ttctaaggaa aatcttgctc atacaataga tgagtatata
      181 gaattagacc aattattcaa aggtgcagaa ggatattatg gtatattaaa atcagtttat
      241 aaaaaataat taggagatgg ttttgtgaga actttaataa aacatgggct tatagtagat
      301 ggaaataaaa caccagcata tgaaggagat attttaatag aaaacgagaa aatattaaaa
      361 atatcacaag atttaaacga agaagctgac aaagttatag atgcaaaagg tagagttata
      421 tgtccaggtt tcatagatac tcatagccat tcagacctag ttattttagt taatccatat
      481 aatgaggtaa agattagaca gggaattaca acagaagtgt tgggccaaga tggtatatct
      541 atggcccctc tacctcaaga gcatatatct tcttggagaa aaaatttagc aggtctagat
      601 ggtgaaagtg atgaaattga ttggaaatat gaaacaacag agaattattt aaaaatgatg
      661 gattacaatg gggtaggttt aaatgaaact tatttagtac ctcatggaaa cgttagaatg
      721 gaagctatgg gacttgaaga tagacctgct actaaagaag aaattcaaaa aatgtgtgaa
      781 atcacagaga gagaattaaa agcaggagct ataggattgt ctactggact tatatatata
      841 ccttgtgcat actcattgac tgaagaaatt atagagatgt gtaaggtagt agcaaaatat
      901 gacggagtat ttgtagttca ccaaagaagt gaagcagata caattttaac ttcaatggaa
      961 gaaatcatag aaattggaaa acaatcagga gtaaaagttc atttttcaca ttttaaggtt
     1021 tgtggtaaag caaactggaa gtatatacca caagttatag agcttttaga aaaagcagaa
     1081 aaggaaggaa taagagtatc ttttgaccaa tatccatatg cagcaggaag tacaatgctt
     1141 ggagtagttt tacctccttg ggcacattct ggaggaactg ataagttaat agaaagatta
     1201 agtgatgaaa acgaaagagc aaaaatgaaa aaagatatag caaatggaat agaaggctgg
     1261 gataatttta tagaatttgc tggaatagac caaatatttg taacaagcgt aaaaacagaa
     1321 aagaataaag atacaattgg taagagctta ttagaaattg gaaaaatgag aggaaaagac
     1381 ccattagatg caacgtttga tttattaaaa gaagaagaaa atgcagttgg gatggtagat
     1441 ttctatggat tagaagaaca tataatagga ttcatgaaaa gagacgagca aaatgtgtgt
     1501 acagatggtc ttttagcagg taaaccacat ccaagagctt atggttcttt ccctaaaata
     1561 ctaggaagat atgttagaga gttgaatgtg ttaacaatcg aagaagctgt ttataaaatg
     1621 actaaaaagg cagctacaag ctttagtata aaagatagag gcgaattaaa agaaggatat
     1681 tttgcagata tagttatatt tgacaaggat acagttagtg gttgtgatga ttttatcaac
     1741 tctatgcagt accctacagg tatagattac gtaattataa atggaaattg tgttgttgaa
     1801 gaaggaaaat acaataaaat taaagcagga aaagtactta aaaattaaat cttagcttta
     1861 aggaggaata aatttatgat tttagtgggg aatgggaggc taataagcca agattcttta
     1921 aatccctata tggaagatgg atgtgtagta ataaatgata atctaattga agatataggt
     1981 actacagaag aaatgaaagc taaatatcca gtacatgaat ttatagatgc aggcaagaaa
     2041 ataatcatgc cgggtctaat aaattcacat atgcatatat acagctcatt tgctagagga
     2101 atggcagttc caggaaaacc ttctgagaac tttttagaga tactaaataa tttatggtgg
     2161 agacttgaca aacagcttac actggaagat acaaaataca gtgcatatgc aacgtatata
     2221 gaatgtataa aaaatggtgt aacaacagtt tatgaccatc atgcaagtcc aaatgcaatt
     2281 gaaggcagtt tgtttacaat atcagatgtt gcaaaggatt tgggaattag aacgtgtctt
     2341 tgttatgaag tgtctgatag agatggtgga aatacaatcc aacaaggtat caatgaaaac
     2401 gtaaacttta taaaatacgc tcaaaaagat gatacagaca tgataaaagg aatgtttggg
     2461 cttcatgctg catttacact atctgatgat acattaaata aatgtgcttc agagatggct
     2521 ggtcttgata caggttacca tgtacatgtt gctgaaggac tagcagactt acaatataat
     2581 gtagataaat atggaaaaag ggttttagaa agattcaatg attttggagt actaaaagaa
     2641 aaaacaatag cagttcattg tatatatata agtgcagaag aaatcagtct tttaaataaa
     2701 acaaatacat ttgtagttca taatccagaa tctaatatgg gaaatgcagt tggatgttct
     2761 cctgcaatag agttattaaa aaggggagtt acagtaggat taggaactga tggatatact
     2821 agcgatatgt ttgaatcatt aaaagttgcc aatataattc acaaacatca cttaaaagac
     2881 ccaagagttg gatttgaaga atcacaaaca atgttatttg ataacaatag aaaaatagct
     2941 aaagcttatt ataaaaatga cctaggagta cttaaaaaag gtgcttatgc agatgttata
     3001 gtagttgatt atataccaca tactccattg acagaaaata caataggggg acacactatt
     3061 tttggaatga gtggaagaag cgtagataca actattataa atggaaaggt acttatgaaa
     3121 gatagaaagt taataaacat agatgaagaa agtattttag caaaaagtag agaattaagt
     3181 caaaatctat ggaatagaat ttaaataatt gatattaata tacaagtaag aaacaagaat
     3241 ttagatgtta aaaacagagc ttaaacaaaa ataggaattt aaatgaagta tataaataaa
     3301 aaatatacgg aatagaatat taaattaaat atagaaggga gacccaaaat gtctcgagat
     3361 aaaagcccta aaatagctcc agtagatgaa aggttacctg tatccaaatc ttgggtattt
     3421 tcactacagc acgttttagc aatgtgtgca ggagcagtag cagttcctat gatgatagga
     3481 agtgctgcac gactagatca tgctagtatt gtttttttaa taaatgcttg cttgtttatg
     3541 gcaggtatag gaacactcat acaaagtgtt ggtataggaa atattgtagg tgctaaaata
     3601 ccagtaatag aaggaactag ttttgccgct gtggcaggta tgacagcaat agcctcatca
     3661 tatagtgatt cacaagttgc aatacagaca gtatttggct ctgtaatagt agctggattg
     3721 ttttgtatat tacttgctcc agtatttgaa aaattaataa gatttttccc tagtgtggtt
     3781 actggaacag ttgttatggt aataggtatt tcactatttc cagttgggat aaaatggata
     3841 actgacaaca aattggaagc ggttgaacca aactcattag cattagcttt agcagtattg
     3901 gcaataactt tactttcatt taaattctta aaaggaattt ggaatagtac agctatattg
     3961 gtaggtatag tacttggtac actactttcc atggcagttg gaatggcaga ttttagtgca
     4021 gtaaatggag ttggttggct taacttaaat gtacctttaa aatttggaat gcctagattt
     4081 gaaccatctg caatactatc attgtgtttg attatgttgg tacttatgac ggaatcagtt
     4141 ggtaatatga tagctattca tgaaatggta gacaaagaag taacaggtaa aaatattaaa
     4201 agaggtctat ttggagatgg aatatctaca gcattatctg gaatattcaa ttcatttcca
     4261 ataactccat ttggtcaaaa tacaggaata gtaggtctta caggtataaa atcaagattt
     4321 atagcagttt acgcaggggt tatattgcta gtacttggat tcttaccaaa atttgcagca
     4381 ataataggag caatacctaa gcctgttctt ggaggcgtag gatttgcaat gtttggaatg
     4441 gtaatggttg gaggagtaaa aactttaagc aaagtgaaat ttgatggaac taaaaatggg
     4501 gttatagtag cagttagtgt tggtttgtct atgataccac taacaaatcc tgatttctat
     4561 cataatttcc caacttgggt acaaacaata ttccatagtg gaataacaac aggtagttta
     4621 tcagcagtgc ttttaaacat attcttcaat gaactaggtt ctaaaaaaga aaaaaataat
     4681 aaagttgaag aagcaaaata aaaataaaac aagtaaaaat ctatattgac ttaaagtcat
     4741 taagcattaa taaaaataat tcagagatat taataattca acaaaactat cttatatttt
     4801 aaataatata aaaaagtaaa taaataacga atacaaataa aagaatacaa tctaaatatt
     4861 aaaaatattt aatttaaggg agagataata atatggctaa tttaaaagtt aatttatatg
     4921 gaatggaatt tttaaaccca attatgacag cggcaggacc tggagcaaaa gatggtgctc
     4981 tctgtcaaga ggcagcaaaa ggtggagcag gagctatatg tacaaaaact atatctgtat
     5041 taccagctga tgtaccaaga ccatgtatgg caaaaactaa tagtggtttt ttaaacacag
     5101 aattatggtc agagcttcca aaagagcaat ggatagagga agaatacaaa aaggtaaaag
     5161 aaacagggct tccagtaata gtaagtatgg gatattcagc agagcaaata gctgaggttg
     5221 ctcctttagt aaagccttat gcagatgcag ttgaattatc tactcattat gtaggtacag
     5281 acgttgcacc aatagtaaat gctttaaaag ctgcaaaagc tgttttagat gtaccagtat
     5341 ttatgaaaat gagtcctcat actgacattc aaactattgc aaaagctgtt gaagaagcag
     5401 gtgcagatgg acttgtaatg ataaactcat ttggaccatg tatggcaata gatgtaaata
     5461 caggataccc tataatggga agtaaagctg gatacggatg gttatcaggt gcaccaataa
     5521 gacctctagc aataagatgt atatatgaag cttctcaagt tgtaaatata ccaataatcg
     5581 gtgttggtgg aatcacttgt ggaaaagacg tggctgaaat gatgatggct ggagcaagtg
     5641 ctgttcaagt atgtacagaa gcaatcctta aagggccttc tatctatgga aaaattgcta
     5701 aagaattaaa tgaattttta gatgagaatg gatatgctga tgttaatgaa ataaaaggat
     5761 taactcataa gaaaatggca gctagagggt ttagaactca ttcagtagct ccagtagtgg
     5821 atgataacaa gtgtatcaaa tgtaagcaat gtgtaacaag ctgtgtatac gaagctttag
     5881 aagttacaga taagttgaac atagatgcag acaagtgttt tggatgtgga ttatgtgtaa
     5941 ctagatgtcc aaaaggagct ttaagcatct gctacaatca agtagaagct atgaatgagt
     6001 aataattata tagaattttg gtaattaact tagagtaata ggtattagga taataaaata
     6061 tatcagataa acaaacacgc attatgcatt aataatacag tctaaagtta ataattttat
     6121 tatattgatt taactaaata ttagtttagc tgaatattaa tttaattagg tattggttta
     6181 atcttaatat agatttacta cgtataagtg aactaactag attactttaa gttaattttt
     6241 tcatataaaa taaattgata cattacaagt aaggtggagt ctatatggga attgttttaa
     6301 aaggtgggaa agtagtaaca agtgaaaaaa cttactgtgc agatgtaaga atagaagatg
     6361 aaaaaatagt cgatataggt cttgatatat caaaagaaaa tgatgaaata gttgatgtta
     6421 gtgattctta tgttatacca ggaggtatag atacacacac acactttgac ttagatacag
     6481 gcgttactaa gacagcagat aactttgaga caggaacaaa agctgctata gtaggaggaa
     6541 ctactactat attggatttt gctacacaag ctaaaggatg ttctttgaaa gacggattag
     6601 aagaatggca tgaaaaagct aaggataaat catattcaga ttatggcttt catatggcta
     6661 taacagattg gaatgactcg gtttgtaatg aaatggagga tatggtgaaa gagggagtat
     6721 cttcatttaa actgtatatg gcgtataaag gaagccttca agttgatgat ggtgtaatct
     6781 ttgaagcatt gagaaaagca gaagagatag gagggattat tggtttccat tgtgaaaatg
     6841 gggacataat atgtgaactt gtggataaag caaagtcaga aaataattta tctccaaaat
     6901 atcatcaatt aactagacca gctataatgg aggcagaagc tacatcaaga cttatgaaga
     6961 tagctgaagt tgcaaactct aaagtatatg tagttcactt aagctgtaaa gaatccttag
     7021 aggaggtttt aaaagctaag caaagaggtg tggatgtaat agttgaaact tgtcctcaat
     7081 accttctatt aaatgatgaa ttatatgatt tagatggatt tgaaagtgct aagtatgtaa
     7141 tgtcaccacc tttaagaaat aaagaaaata atgaaatatt atggaatgct attttagata
     7201 atcaaataga cactatagga acagaccatt gttcttttaa ttataaggga cagaaggaac
     7261 ttgggataaa cgatttcagt aagataccaa atggttctcc aggtgtagaa cataggatga
     7321 gtcttttata cacatatgga gtctgtaaaa ataaaatatc tatcaacaag atggtagaag
     7381 taacatcaac taatgcagca aagttgtttg gaatgtatcc taataaagga actatagaaa
     7441 taggcagtga tgcagatata ttagtcttaa atccaaacaa aaaatccaaa atagaagcaa
     7501 aaaaacaatt acaaaatgta gattatacac cttatgaagg gtatgaagtg gattgtcaaa
     7561 tagaacatgt ttatttaaga ggaaaagaag tagttagaga tggagaatta atttgtaata
     7621 ctccaagtgg gagatatata aataggcgtc taacaaaggg ggctagagaa tggtagaatt
     7681 tatattaaat ggagagaaag ttgttgcaaa agaaggcaaa aaacttttag acttccttag
     7741 agaagatatg aatataactt cagttaaaaa tggatgttca gaaggtgctt gtggaacatg
     7801 tatggttata atagatggta aagctactaa agcatgtgta tttaaaactg ataaattagc
     7861 tgggaaaaac ataacaacta tagagggatt atcagagaga gaaagagatg tttttgcata
     7921 ttcttttgct agtcaaggag ctgtacaatg tggattttgt attccaggta tggtaattag
     7981 ttcaaaagca cttatagacg ttaataataa tcctactgaa gatgatataa agaaagcttt
     8041 aaaaggcaat atatgtagat gtacagggta tgtaaaaata attaaagctg tcgatttagc
     8101 ggcaaagatt tttagagaaa atatagatgt gccaaaagta aactgtaagg gtctagtagg
     8161 tgaaaatctt cctagagttg atgcagttgg caaagcactt ggatattctg aatatgtaga
     8221 tgatatgaga attgagggta tgatttatgg ttcagcagtt agagctaagt atcctagagc
     8281 tttagttaaa aaaatagata tagaagaagc aaaagcttat ccaggagttg ttgcagttct
     8341 aacagctaaa gatattccag gaactataaa ggtagggcac ttaaagaagg attgggatac
     8401 tttaatacca gaaggtgaaa taactagata cttaggagat ggaatagttt tagtagcagc
     8461 agaaactaga gaagcgttag aagaggccaa aaagctagtt aagatagatt atgaggaatt
     8521 aacaccatta agtactacag cagatgcact tagagaagat gctccaaaga tacatgagac
     8581 agggaatata cttgttaagg aacatttagt aagaggaaac gctaaagaaa agatagaaaa
     8641 ttcaaaatat gttgtaacta aaagatatac tacacctgct acagaacacg catttttaga
     8701 gcctgaatgt gcagtagctg gtccttatga agatgatgga gtaataatct attcaacaga
     8761 ccaaggggtg tttgccactc aacatgaatg tgcagatatg ttagggttac catatgagaa
     8821 ggtaagagtt ataaacaaaa tggtaggagg agggtttggt ggaaaagaag acatgagtgt
     8881 tcaacaccat gcagccttac ttgcatacca tacaaagaga tttgtaaaag tacagttaac
     8941 aagaaaagaa agtattattg ttcatccaaa acgacatgcc acagaaatgg aatttacgac
     9001 agcatgtgat gaaaatggat acttaacggg tatgaaggca acgataatat ctgacacagg
     9061 tgcatatgcc tctcttggag gaccagtgct acaaagactg tgtactcatg cagcaggacc
     9121 atacaattat caagatattg atataacagg aacagcagta tatactaata accctcctgg
     9181 aggagcattt agaggatttg gagttacaca gagttgtttt gcaactgaat gtaatctaaa
     9241 tttacttgct gaaatggttg gaatatctcc ttgggagata agatataaaa atgcaataag
     9301 acctgggcaa gttttaccaa atggtcaatt tgcagatgaa ggaacagcaa tggcagagac
     9361 actagaagct gtaaaagaag agtatgaaaa gcatccacgt gcaggtatag cttgtgctat
     9421 gaaaaatagt ggggttggag ttggtatacc tgatgttgga agatgtagat tggtgataaa
     9481 agatggcaaa gttcacataa gaacaagtgc ttcttgtata ggtcaagggc ttggtactgt
     9541 tatgactcaa gtagtttgtg aaactgctag cctacttcct gaaaatatag tgcatgaagt
     9601 tccagatact catttaacac cagactctgg taatactacg gcttcaagac agacagtatt
     9661 tacgggagaa gctactaagg ttgcatcaaa taagttaaaa gaagctttag actctggcaa
     9721 aactttactt gatttagagg gagaagagtt ttatggagaa tatgaaagta ttacagataa
     9781 aatgggttgt gacaaagaaa atccagtaag tcatgttgct tatggatatg caacacaagt
     9841 tgtaattctg gatgaaaatg gaaaactaga aaaagtagta gcagcacatg atgttggaag
     9901 agctataaat ccaatcagtt tagaaggtca aatagaaggt ggagtagtta tgggtcttgg
     9961 atatgctcta accgaagatt acccagtagt caactctgtt cctacagcta agtttggtac
    10021 attaggttta tttaaatcta cagatatacc agaaatacaa gctttaatag ttgaaaagaa
    10081 caaagattta ttggcttatg gggcaaaagg tgtaggagaa atttgtacag ttcctactgc
    10141 accagcagta caaaatgcct attatatatt tgataaagaa tttagaactg agctaccact
    10201 acaaaatact ccatatagta aaaaaaagta acaaaaaaaa gaaatattaa ataaaaaaat
    10261 agatgaaatt gtaaataaaa aaaacccttt tgcataaggt atattttaaa tgatagaata
    10321 tacatataca ggagggtttt tcttttataa aataagttta ataaaattga gaggtgagca
    10381 gaatgaattt taataatagt ctagaattaa ctcagtctca gaagctaata atgactacac
    10441 aacttaagca atcattatca atattaaata tgagtaagtt agagttagaa gaagagataa
    10501 aaagagaagc agagaataac ccattagttg aagtcgagaa aagtggggag ataaattggg
    10561 aagagtatat aaaagacatg gataggtcaa gacgtttaga tagaacagag ataagctata
    10621 atcctgataa tgaagttaat ctagaaaatt tagttaaata ttcgtcaaat ttatatgaag
    10681 atttaaaatt tcagataagc ctgtataaac ttacagacaa agaattggaa gtctgtgagt
    10741 atataattga tagtttagat gaggatggat atttaagaac tgaggaaaaa gtaattgttg
    10801 ataccttaaa gatagatgaa gaactttttg aaaaatgttt aattagtgtt cagcagttgg
    10861 aacctagtgg agtaggggca agaagtcttt cagaatgtct gatgatacaa atggctagtt
    10921 taggaatata taatgaaatt ttagaggaaa tcgttacaaa agacttaaat ttaattgcaa
    10981 ataataaata caaagagata agtaaaaagt acaatatgtc attacaaaaa tgtgttgaac
    11041 taataaacat aattaagaca ttagacccca aaccaggaag aacttgttca gtagagaaaa
    11101 gtgtatacat tcaacctgat gtaacagtag aaaaaattga tgatgaattt attgtatatt
    11161 taaatgagaa agattcttat caaataagaa tcaatagtta ttataaagac atattaaaaa
    11221 attcacagtc tgatgaaagt gcaaaagaat ttataaaaga aagactaaat tcagcaacag
    11281 gacttatgaa aaatatagag agtaggaaaa ctacagtact taagattgca gaagagatag
    11341 taaaagctca agatgaattt ttgagaaaag ggacaaaata tataaaacct ttgaaaatga
    11401 aagacatagc agaaaaatta gaatttcatg aatctactat aagtagaggt gtaaatggta
    11461 aatatatgct aacacctttt ggcgtatatg aatttaggta ctttttcagt agcgccattg
    11521 agactgagaa taatgaaatg gcatctagta caagcataaa aaaaattata aaggaaacaa
    11581 taaaagacga aaataaaaag aaacctttaa gtgatgacca tatatctaag ttattaaaag
    11641 aaaaaggaat caatgttgca agaaggacag ttgccaaata tcgtgaagaa ttaggaatcc
    11701 catcttcaag taagagaaaa gaatactaat aagtaaaaga caaatatcag tatggtaagg
    11761 ggaaaaggct aattaagaga tgaatgtaga ttgggctaaa aattttataa agtaaaataa
    11821 tagaatatgg aaaagtatta aaatgctata aggtattaaa aaactgggga acattaaaac
    11881 attaagatag caaagagcat aaaaaactaa agaagattaa aacattaaag aacattagag
    11941 ctttgaggaa tattagaact ttaaagaata ttaaaattta taaaatataa aaatactaaa
    12001 aagaattgaa aaactgggga acattaaaat attgtaaaat gctaaaaaac tagaaaacat
    12061 aattaaataa gtgcttataa agaatgatta aattaaataa gtgtttgtaa agaataatta
    12121 gatattagaa aaagaagaat acctagataa ataaactatt attaaattta ataatcacaa
    12181 aaggaaaaca cgtagggtat aaataaaaat cttaactaga taaaagtaaa cttagttgta
    12241 gataaaaata actagaaata aaagtaacta aaaataaaat tatttatagt aataaatatt
    12301 agaatgaaaa tataaaaaaa tatatataaa aagccttata taaaaagaaa aaatactgtg
    12361 atttgaaaat ggtggagaaa tattttataa aataaaaaaa taaaaaagtt tttgaaaaac
    12421 tattgaaatc tatacgcaaa tcctataaaa taaaaatatc ttgtgggact taaataaaca
    12481 ctagggacaa aaaaagtccc gaaaggaaag caataaggag tatgttatga agaacttatt
    12541 agaaattcaa aagaaattga ttccacaggc aattgaactt atggaaagaa gatactctat
    12601 tttaagacaa atatctttaa gtgaaccaat aggtaggaga acattatcaa atgtattaga
    12661 gattagtgaa agaataatca ggtcagaaac tgagttttta aaagaacaag gacttataga
    12721 cgtagctgta tctgggatga ctatcactaa agaaggttct gatttgttag ataaacttaa
    12781 ggacatcatg agtgacatca tgggattgtc taagttacaa gatagagtca gagaaaagct
    12841 tggaataaaa agggtaatat tagttcctgg aagctgtgat gaaaatggaa gtttactaaa
    12901 agatgtaact agatatggat gcgaatattt cttaggtatt ttaaaagatg gagatatcgt
    12961 ttcaataaca ggaggaagca ctatgttgga gttttcaaac gttattaaaa ctgataagag
    13021 atatccaaac tctacaatag ttccagctag aggaagtatg ggaactgatg tagagatgca
    13081 atctaacaat atagttgctt caataagtaa gaaattacat tctaattgca aacttttaca
    13141 tataccagat gagttggatg aaagtgcaat gaaaactctt agccaagtgc cagagattaa
    13201 gacaactttg gagtatatta aaagaacaga cattttagtt tttagtatag gtagagcaga
    13261 tgtcatggca aagagaagaa aacttccaga agacaaggtt gatgagatat tatctaaaaa
    13321 tgcagttgga gaagcatttg gatattattt caacaaagat ggagaaatag tttacaaact
    13381 gaacactgtt ggaattgatt tagaaacagt taaacaagta aaagaaacta ttgcaatctt
    13441 tgcagggact gaaaaagtag aagccttatt agctatatca aatatgcata aaaacatggt
    13501 tcttgtaaca gatgaagaaa gtgcttataa gatattgtca ttattataaa agtcaagacc
    13561 tagcacagag ctagaaaaca tacatttact ttataggagg tagcgaaaat ggttaaagta
    13621 gctataaatg gatttggaag aataggaaga ttagcattaa gaaaaatgat ggagcaacaa
    13681 gataagttcg aagttgttgc aataaacgac ttaacagacg ctaaaatgtt agctcattta
    13741 tttaaatatg atacagctca aggaagattt aacggagaaa tagaagttaa agaaggtgct
    13801 tttgtagtaa acggaaaaga aataaaagta acagcagaaa gaaatcctgc tgacctacca
    13861 tgggctgaat taggagtaga tatagtatta gaatgtacag gattctttac atctaaagat
    13921 aaagctgaag ctcatataca agcaggggct aagaaagttg ttatatctgc accagctact
    13981 ggagatttaa aaacaatagt attcaacaca aacagtgaca tattagatgg ttctgaaaca
    14041 gttatatcag gagcttcttg tactactaac tgtttagctc caatggctaa agtattaaat
    14101 gataaatacg gtatagaaaa aggattaatg actactatac atgcatacac taatgaccaa
    14161 aatactttag atggtcctca tccaaaaggt gatttaagaa gagctagagc tgctgctgga
    14221 aacatagttc caaacactac aggagctgca aaagcaatag gcttagtaat accttcatta
    14281 aaaggaaaat tagatggggc agcacaaaga gtaccagttg taactggttc aataactgaa
    14341 ttagtttgta cattaggaaa aaatgttaca gttgaagaaa taaatgcagc tatgaaagaa
    14401 gcttcaaacg aatctttcgg atatactgaa gaaatgttag tatcttctga cataatcgga
    14461 ataagctacg gttcattatt tgacgctact caaacaaaag ttatggaagt agatggaaaa
    14521 caattagtta aagttgtttc ttggtatgat aatgaaatgt cttatacttc tcaattaata
    14581 agaactttag gatactttgc acaattagca aaatagtcag ttttgagaaa atagaaaagt
    14641 agattgacaa agtccgagtt tgtagtttaa tactacggac tcaggacttt ttttaagaaa
    14701 aaatatataa aatacaccta aataatgtta catactatat ataaaaatat gataatatga
    14761 tatatttaat agggtaaaag tatgtactat attatacttg gcaataaaaa attactttat
    14821 taaaaggaga gatatttatg tcaatgctta acaaaaaaac tatagaagat attgatgtgt
    14881 gcggaaaaaa agtattagta agatgtgact ttaatgtgcc attacaagat ggtgtaatta
    14941 ctgatgaaaa tagattgaac ggagctttac caactattca atatttaatt tctaaagggg
    15001 caaaagttat attatgctct cacttaggaa agccaaaagg agaagctaaa ccagaattat
    15061 ctttagcacc agtagcaaag agattatctg agatgttagg aaaagaagtt gtatttgcag
    15121 cagacgataa tgttgtagga gaaaatgcaa agaaagcaac tgaaaaaatg gaaaacggag
    15181 atgtagtatt attagaaaat actagatata gaaaagaaga aactaaaaac gaagagaatt
    15241 tctctaaaga actagcatca cttgctgaaa tatttgtaaa tgatgcattt ggaactgctc
    15301 atagagcaca ttgctcaaca gttggtgcag gcgagttttt acaagagaga gtttgtggat
    15361 acttaattca aaaagaatta aaattcttag gagaagctgt tgcaaaccct gtaagaccat
    15421 ttacagctat acttggagga gctaaagttt ctgataaatt agctgttata aacgaattat
    15481 tagaaaaagt tgataatcta ataataggtg gaggaatggc ttatacattc ttaaaagctc
    15541 agggttatga agtaggaact tctttacttg aaatagataa agttgaatat gctaaagaaa
    15601 tgatggaaaa agctaagaat aagggtgtta acttattatt accagtagac gttgttatgg
    15661 cagaccactt tgcaccagat gctactccaa tagtaacaga agatgctaat gtaaaagaag
    15721 attacatggg actagatatg ggacctaaga ctatagcaaa ctttgtaaaa actataaaag
    15781 aatcaaaaac tgtagtatgg aatgggccaa tgggagtatt tgaatttgaa aactttgcaa
    15841 atggtacatt atcagtagct agagctatgg cagagttgac tgatgcaact actgtaattg
    15901 gcggtggaga tagtgcagca gcagttaatc aattaggatt tggtgacaag atgactcacg
    15961 tatcaacagg tggaggagca tcactagaat ttttagaagg taaagaatta ccaggtattg
    16021 cagcattaga taataaataa actaatatat agaggagatt gtaaaatgag aaaacctata
    16081 attgcaggaa actggaaaat gcataaaaca ataaaagaag ctttagaatt tgtaaatgaa
    16141 gttaaagata aagtaaattc tgataaagta gaagctgtta tatgtgcacc atttacttta
    16201 ttaaaggatt taaaagaagc tactaagggt acaaatataa agataggtgc tcaaaatatg
    16261 cactttgaag aaaaaggagc atttacagga gaagtttcac ctcttatgtt aaaagaaata
    16321 gatatggact atgttgtaat aggacattca gaaagaagac aatacttcaa tgaaactgat
    16381 gaaactgtaa acaagaaagt tttaaaagca ttagaagtag gaatagaccc aatattatgt
    16441 gttggagaaa ctttagaaca aagagaagct ggaaaaacta aagatgtttg taaagttcaa
    16501 gttgaaaaag ctttagaaaa tgttcttaaa gatgatttag caaaagtagt tgttgcatat
    16561 gagccaatct gggctattgg aactggtaaa actgcaactg ctgaagatgc taatgacgtt
    16621 atatcttata taagagaagt tataaaagga ttatatggag aattagctaa tgaagttaga
    16681 atacaatacg gtggaagtgt taaaccttca aatgtagccg aaataatggg tcaaagtgat
    16741 atagatggag ctttagtagg tggagctagt ttagctagta atgattacct agaccttgtt
    16801 aatttctaag gagtgaaatg atgaaaaaac cagttgcttt aattattatg gacgggtttg
    16861 gatacaataa ggatgttaag ggaaatgcaa ttgctgagtc caaaactcct aatttagata
    16921 gaataaaaaa agagtatcca aacacattga taaatgctag tggtcttgat gttggtctac
    16981 ctgatggtca aatgggaaat tcagaagttg gacacactaa cataggtgca ggtagaatag
    17041 tatatcaaga tttaactaga ataacaaaat ctataaaaga tggtgacttt tttactaata
    17101 aagttttatg tgaagcaatg gataatgcaa aagaaaattc tcttcatgta atgggacttt
    17161 tgtctgatgg aggagtacat tcacatatag accatcttaa ggctataatc aaaatggcaa
    17221 aagataaagg cgtacaaaag gtgtatgttc acgcatttac tgatggtaga gatactgacc
    17281 ctcaaagtgc attagaatat gccaaagaag tacaagctag tatggatgaa attggagtag
    17341 gtgaatttgc tacagtttct ggtagatact acgctatgga tagagataag agatgggaaa
    17401 gagtagaact tgcatacaat gccatggtta gaggtattgg tgaaaaagca aattctattg
    17461 aggaagctat tcaaaactct tatgatgatg gaaaaaatga tgagtttata atgccaacag
    17521 ttattatgaa agatgataaa ccagttggaa gtataaagga aaatgattca attatattct
    17581 tcaactttag accagataga gctagacaaa taactagagc tttagtttgt gaagagtttg
    17641 atggatttaa aagagaagat attaaaaact tctttgtatg cttaacagag tacgatataa
    17701 ctatagagaa tgtgcatata gcatttggac ctcaatcttt agccaacact ctaggtgagt
    17761 atctagctaa aaatggaaaa actcaactta gagctgctga aactgaaaag tatgcacatg
    17821 taactttctt cttcaatggt ggagtagaag agccaaataa aggtgaagaa agactattga
    17881 taccttctcc aaaggttgca acttatgatt taaaaccaga aatgtcagca tatgaattga
    17941 cagataaagc tttagataaa ttaggagaag ataagtttga ctttatagta ttaaactttg
    18001 caaatcctga tatggtaggt catacaggaa gtatagaagc ggctataaag gctgttgaaa
    18061 ctgtagatac ttgtgtagga agattgatag ataaaatagt agagttaggt ggaagtgcaa
    18121 taataactgc tgaccacgga aatgctgaat atatgttaga cccagaaaca ggaaaaactg
    18181 taacggctca ctctattaat ccagtaccat ttattgtagt tggtcaagaa tatgaaagtg
    18241 caaaattatt ggatggagga agattatctg atatagcacc gactgtttta gatatgatga
    18301 agttagaaaa gccagaagaa atgactggtc attcacttat ttcaaaatag tcgattctga
    18361 taagtagaga ataatatata aactacaata taaatttaaa aataatgcag tttaggttta
    18421 ttttgtaaag tttagacttt atgacagaag atttagattt tgtaagtttt ggatattgta
    18481 aggtgtagat gaagatatat tttataaaat ttacaagata gacaatttat aaaataaaaa
    18541 attataatat agataataat gtaaataatg atataaataa taacaagata gacaataa
//
LOCUS       DAMKMI010000075        18591 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMKMI010000075 DAMKMI010000000
VERSION     DAMKMI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.076"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            200..1417
                     /locus_tag="PC429_002310"
     CDS             200..1417
                     /locus_tag="PC429_002310"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF3847811.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1512..1892
                     /locus_tag="PC429_002311"
     CDS             1512..1892
                     /locus_tag="PC429_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF3847812.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2176..2658
                     /locus_tag="PC429_002312"
     CDS             2176..2658
                     /locus_tag="PC429_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3847813.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2679..3494
                     /locus_tag="PC429_002313"
     CDS             2679..3494
                     /locus_tag="PC429_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3847814.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3547..5097
                     /locus_tag="PC429_002314"
     CDS             3547..5097
                     /locus_tag="PC429_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3847815.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5194..6546
                     /locus_tag="PC429_002315"
     CDS             5194..6546
                     /locus_tag="PC429_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF3847816.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6677..7435
                     /locus_tag="PC429_002316"
     CDS             6677..7435
                     /locus_tag="PC429_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847817.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELFNLLHTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7482..8201
                     /locus_tag="PC429_002317"
     CDS             7482..8201
                     /locus_tag="PC429_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF3847818.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8325..9725)
                     /locus_tag="PC429_002318"
     CDS             complement(8325..9725)
                     /locus_tag="PC429_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF3847819.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9729..10637)
                     /locus_tag="PC429_002319"
     CDS             complement(9729..10637)
                     /locus_tag="PC429_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3847820.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKMIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10898..11545
                     /locus_tag="PC429_002320"
     CDS             10898..11545
                     /locus_tag="PC429_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3847821.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11869..14508
                     /locus_tag="PC429_002321"
     CDS             11869..14508
                     /locus_tag="PC429_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF3847822.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14566..15669)
                     /locus_tag="PC429_002322"
     CDS             complement(14566..15669)
                     /locus_tag="PC429_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF3847823.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIHLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16030..16851
                     /locus_tag="PC429_002323"
     CDS             16030..16851
                     /locus_tag="PC429_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3847824.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16930..17141
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17228..18544
                     /gene="argH"
                     /locus_tag="PC429_002324"
     CDS             17228..18544
                     /gene="argH"
                     /locus_tag="PC429_002324"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF3847825.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7099 a         2151 c         3289 g         6052 t
ORIGIN      
        1 atatttttaa attatcattt aatgtaattt aaatgaattg aagatatatg tttattgtat
       61 gcaattgttg tatttttagg ttttttgtga ttaattaaaa aattataaaa atcgtttaca
      121 tgttgatttt taaaaaaaaa agatatagaa ttatatagaa agtaataaac aaaaattgga
      181 tttacgggag tggaatgaaa tgatgaatgt taccttaaca gatgtaaaag aggctagaga
      241 gaccattaag aacatagtaa aaagaactga cttacttgaa agtgttaaac ttagcgaaaa
      301 aacaggagca aatgtgttct ataaatgtga gaatcttcaa aaaacaggtt cttttaaatt
      361 aagaggagct tgtaacaaaa tagcgagttt aacagatgaa gaaaaggcaa atggagttat
      421 agcttcaagt gcagggaatc atgctcaagg agttgcattg ggagcaaaaa tgactggtat
      481 aaaatctaca atagtaatgc cagcaactgc gcctttagca aaagttagtg caactaaagg
      541 ttatggagca gaagtagttt taaatggtgc ggtttatgat gatgcatatg caaaagctgt
      601 tgaaatacaa aaagaaacag gagcaacttt cttacatcca ttcaatgata agtatgttat
      661 agcaggacaa ggaactatat ctcttgaaat tttcgaacaa ttagacaata aagttgatac
      721 tatactatgc ccagttggtg gaggaggaat catctctggt gttgctgttg cagcaaaagc
      781 attaaatcct aatgtaaaaa tagtcggtgt tcaaactgca aatatacctt caatgaaaga
      841 gtcaataaaa aatggaaaag taacaacagc atttaatgat actacaatag ctgatggtat
      901 agctgttaaa actccaggag atttaacttt tgaaataata aatgaattgg ttgatgaaat
      961 agtagtggtt gaagagacag aaattgcaga atcaatatta tttatgatgg aaagtcaaaa
     1021 aatagtttca gaaggtgctg gtgctgtatg tacagctgct atattaagtg gtaagtatgt
     1081 accagcaaaa gatgaaaatg tagtttgtat aatatctggt ggtaatatag acataaatac
     1141 gttatataga ataataggtg ttgctttagc taaagaagga agaagatatt catttagtac
     1201 aattatggaa gataagccag gaaactttgc tgagttaact agaataataa gtgagaatgg
     1261 tggaaacatt ttaagtgcta atcaaggtaa attatcagca ggggaagctc taggaaaaca
     1321 aagtgcggaa tttatattag agacaataga ttatgaccat atagctagaa taaagaaagc
     1381 tatagaagaa aaaggtttta aaattataga attataataa aaagtgtata taatatacaa
     1441 gaaaaatagt ataatagtgt atgaagatac attttatgtg tattagatta acgaattagg
     1501 aggattttaa aatgaaacat gaagtaatac atactaatga tgcaccagca gctttaggac
     1561 catattctca agctataaaa gctggaaact tgttatttgt atctggacaa gttcctcttg
     1621 taccagagac tatggaagta gttgaaggag atgtacaagc tcaaactgct caatctttaa
     1681 aaaatcttaa agctatatta gctgaaagtg gagctgattt ttcaaatgta gttaagacta
     1741 cagtatttat aaaagatatg aatgaatttg gggctataaa cgaagtttat gctgaatatt
     1801 ttggagaaaa taaaccagca agagcttgtg tagaagttgc tagattacca aaagacgtta
     1861 aagttgaaat agaagtaata gctgtattat aaaaatacaa tatttttaat ttgaaaaggc
     1921 tgaaaaacta tattctagtg attcagcctt tttataaaaa aataaatcac aataaagaac
     1981 atgataaata ttgtcgaatt aaaaaataaa attaaaataa atattgcaat atttttgaga
     2041 aggttttata atgagtatat aatttgaaac gtgtaactat taaattaggc atgagttctt
     2101 ggatattttt atcctgagga ttcatgcctt ttttgtattg aaattcaaaa atctatttaa
     2161 taaggaggaa gtactgtgtt aaattttttc aagaaaaata aaagctataa gttacatgca
     2221 gtagtgagtg gtaacagcat taatattgag aaagtaaacg attctgtgtt ttcaaaaaag
     2281 ttaatgggag atggagttgc aattattcct aatagtaatg ttgttgtagc cccttgtaat
     2341 ggtaaagtaa ctgtgcttac agaatcaaag catgcctttg gtatggtctc agatgagggt
     2401 gttgaaatat tagtccacat aggaattgat acagttagtt tacagggtga ggggtttaaa
     2461 aatgaagtgt ctcaaggaga tactgttaaa aaaggtagtc caataataag ttttgaaaga
     2521 gaaaaaataa attctcaggg aattgattgt acaacaataa taattgtatt aaatcatagc
     2581 gaattttcag aaataaattg catggtagaa aatgaagttg ttgcagggca agatactgtt
     2641 attgaaatta tgaaataata gaaagggatt aattcaatat gagagtagta aaaatcttta
     2701 ataataatgc attatctaca gtaactaatg ataggaaaga agctatttta ctaggtcttg
     2761 gtataggttt taataaacgt ccaggagata aagttaatga agataaaata gaaaagattt
     2821 attatgttca agaccacatg caaacaaaat ttttagaatt attaaaaaat gttagtccag
     2881 aagtgatgga tgcttcacaa caaattataa gtcttatacc aggaaatgag ggaaaattta
     2941 ataataaagg gatattatct ttagtggaac atattagttt tgcaatagat agaatgaaaa
     3001 ataatgtttt tttacctaat ttaatgcttt tggatataaa gatgatgtat tcaaaagagt
     3061 ttgaattagg agtaaaagct ttagaaatta tatatagagt atgtcatatc catttgcctg
     3121 aagatgaagc aggttacata gctttacatt ttgtcaattt acaatcaaat gataatttgg
     3181 catatgatac tttaaaattt gtaaaaggta gtattgattt gattaaagaa tgttatggat
     3241 tagaattaga cgaatcgagt ttaagtacat tacgttttag aactcatctt aaatttcttg
     3301 ctcaaagaat ttttaagaat gaaatttgtc aggatgataa aatgattgaa atgtatgatt
     3361 atttaattaa caatcatcct aaaaataaag aatatttaga aaagttaaat gtatatattg
     3421 aaaaggagtt taggtataaa ttagataaac ctgaaaaaat ttatctacta ctacacctaa
     3481 caaaaatctt ataagaatga gatgaattag taatagaaat atatataatt ttaggggggg
     3541 ataagattga aagaaaaact acttggagga ctggaaacat ttgcaaaggc aatggttcag
     3601 ccattaatgt atttatcgag tgcaggaata ttaatggtgg taggtgtact acttacaaat
     3661 gatatgttat taggagtact tccatttttt aaatgggagc caattcaaat atttggaagc
     3721 ctaatttata actgtataat ggctattatt aataatcttg ctgttatatt tgctataggt
     3781 attcctgctg cactagcaaa aaaagagaag cacaaagcag cgttaatagg atttatgagt
     3841 tatataatat accttacagc ttccaacact atgttaacat cactaggtca actagcaaaa
     3901 gaaagcaaga tgcttggact gatagggact ggacaagcta ctattttagg tattcaagtt
     3961 gtagatacaa gtgtatttgg tggaattatt ttagggtata tagctggata tgtatttaat
     4021 cgtacaagca acaaaacatt taaaggagct ttacagatat actcgggagc aaattttagt
     4081 tttatatgca tgttctttgt atccatttta attggagttg tgagtaactt tatatggcca
     4141 ttagctcaac aaggaatagc ttctcttgca aatgtcatca aagatacagg tacatttggt
     4201 ttattcttat atggtttttt agagagaata ttagtaccaa ctggacttca tcatttaatt
     4261 tatacaccat ttcagttttc tgatttagga ggagtgctta aagttggtgc agatacagtt
     4321 tcaggagcct atgctatagt tatgacagaa atgaatttac cagttgagcg tttctcagat
     4381 agtatatact acatggcaac tggatttaca aagatgtttg gttatatagg tataggagct
     4441 gcctttattt atactgcata taagcaaaat aagaagaagg ttaaggcaac gattattcca
     4501 ctaataatta cggcagtatt agcctctgta acagaaccat tagactttat gtttgtattc
     4561 tgtgcaccac ttttattctt tgtacatgca gttatagctg gtctttttat agctttatta
     4621 aaaatatttg atgttactgc attatgtgga gggaacttat tagtatcagt tataatgaat
     4681 actacagtag gcgtagaaaa aacacactgg ccagtaatga tgatattagg acttgcacaa
     4741 attattgtat attttgtagt atttagtttt ttaattaaga aatttaatta taaaactcca
     4801 ggtcgtgaag atgagagtac agctacaggc gaagaacaat caaaacaact taatttagat
     4861 gcagggatag aaaatataat tgatgggtta ggcggaaaag aaaatattaa tacagtagaa
     4921 aattgcatca ctagattacg tgttaatgta aaagatgaaa gtaagattaa tgaagatata
     4981 attaatttaa cgccaaatag cgggattgta aggaaaggaa aagatataca aataattttt
     5041 ggacttcatg tgcatgaggt aagacgtgca gtagaagatt ttttagaagc ttattaaaga
     5101 tatatattta tatagcaagg gtaaataaca gctttaagtg ctgtttacta tttattaaag
     5161 atacatataa tttatagagg aggaataatt attatgatta ttactattgt tggtggagga
     5221 agcacattta ctccaggtat tgttaagtca attgcattaa gagaaaaaga gttagagctt
     5281 gaggaaattc gtttatttga tattgataaa aaacgtcaag ataaagtagc tgtagttgtc
     5341 aactggatac taaaagaaga tgttaaaaca gatgttaaac taactgtaac taattgtgaa
     5401 aaagaagcgt atacagatgc tacatttata tttgcacaaa tgcgtgttgg taaatatgaa
     5461 atgcgtgagc aggatgaaaa aattccattg aaatatggtt gtgtaggtca agaaacttgt
     5521 ggttgtggag ggatggcata cggactgcgt acaatacaac caatgataaa ggttattgat
     5581 gatgtagaga aatatgctaa aaaagattat tggatattaa attactctaa tcctgctgca
     5641 atcgtttctg aagcttgtcg taagttaaga ccaaatgcaa ggattataaa tatctgtgat
     5701 atgccaatag ctattataga tatgatagcg gggtcattaa atatcaatga cgttcataat
     5761 atacgctatg attattttgg tctaaatcat tttggctggt ttacttcaat agattataaa
     5821 catagagact taatggaaga gattaaggaa tacataaaag aaaataaaat attattacca
     5881 aaatcttata tagaaaactt aaataaaagt cgtagtacag gaaatcgcca tgctgtaagt
     5941 agctggatac atgtttggga atctgtttat acaatgttag aaagcttccc aaaatatctg
     6001 ccaaacactt atttaacata ctatctttta agtaaagaaa cagtggaaca ttcaaacata
     6061 aatcgtactc gtgcaaatga ggttatggaa agccgtgaaa aagacttatt tgaaggtgtg
     6121 agacattact tagaaactgg agaaatttct gaaaaagcat tttatgcagg ttcacatgga
     6181 gattggatta gtgatttggc agtttcaatt aaaaatgata cacgtagtcg ttttttagtt
     6241 ataactgaaa atcgtggagc tataccaaac atgccatatg atgcaatggt agaaattcca
     6301 gcatatattg gtaaagatgg tccagaagtt attgctcgag gtccaatacc tctatttcaa
     6361 caaggattaa tgatgcaaca acttaacagt gaaaaattga tagtagaagc ttatatagaa
     6421 aatagttatg aaaaagcttt acaagcattc acattaaata aaactattcc aagtatgaat
     6481 gttgctaaag aaattttgga tgaaatgata gaagttaata aggaatactg gccagaatta
     6541 agatagtagg ataaaagaaa gggtttttat ttaatcatta gatatgtttt cagagtcttt
     6601 tatcaaaatg tattcacaaa catatggcaa tatattaaaa taaagagtga tatttttaga
     6661 agaatggggt gatgcaatga acattataaa taaaattttt tggcaagaag ataggattgg
     6721 catgattaca aataatttag aggcagatac acatagccac tgtatgttac aactgttctt
     6781 agggatagaa gacagcattg aaataacagt taatgaaaaa ttagttaaat gtaattgtat
     6841 catagtagat aaaaatatct ctcattcatt ttcagcaaga aaaaaagttt attactctgc
     6901 tattattgaa cctacatcta tatatgcaga acagcttacc tcaaaaatga atgattttgg
     6961 ctactggata tgtgacaatg atggattaga gaaattacgt caacaaggaa cttttttgat
     7021 atctaactct agtaaagaac aatatttaag atttatggaa atgcttaata actatttgaa
     7081 tatacctata actctcaaac attatgatga cagaattact gaattgttca atttattaca
     7141 tacttgtaat tgtgataatc atactatttc aagttttgca gataaagtgt ctctatcagc
     7201 aagtagatta tcacatctgt ttaaagaaca aatagggata cctttaaaaa gctatatctt
     7261 atttcatcaa atggagtgtg cttttagaga attactttct ggaaaaaatg ttaccgaagc
     7321 ttctatgact gctggattcg atactccatc acactttgca gggacagtta agcgaatgat
     7381 gggaatgcct gtttctttat ctttgaaaga tagcgagttt ttgaaagtat attaaatatt
     7441 ctcgttatat aatgaagttg tactaagaaa ggggctttgt tatgaataaa gaatatttag
     7501 aagaaacaaa aatgttaaat tataatgaac cacaacttaa gcttttggta tcatcaaaga
     7561 attggttgga attagatgat tttcataaaa taaaatctat ttatgagttt gtacagaatg
     7621 atattttatt tggatataat gcttctgata tgttaagcgc aacacaggta ttaaatgatg
     7681 gtatgggaca atgcaataca aaagctactc ttttaatggc attattacgt gcagtgaata
     7741 tcccttgtag gcttcatgct tttgatgtaa caaaagattt tcaacgagga gctacatcca
     7801 aattgatttc actattagca ccaaaatata ttcttcatac ttgggtagag gtattttatc
     7861 aagataggtg gattgctctg gagggtgtca taactgataa aaaatattta gaagcaattc
     7921 agaagaaatt ttttaatcat ggtggaacgt ttaaaaagta tgcaatagct acaaatgatt
     7981 tgaaaaatac atctattgat tgggatggta aagatacttt tatccaaaaa gaagctatcg
     8041 tgtatgatta tggtattttt ccatcaccag acgtattttt ttctactcat tcacaacata
     8101 tgtcaaaatt aaaaaatttc atatatgtac atcttatcag aaaaataatg acaaaaaatg
     8161 tatgtaaggc tagaaacaat tatattgata aaaatgaatg agtgtttaag aggtaactat
     8221 ataggaaata tatagaataa ttaaaactgt actggttaaa atgaataaaa aggagttatc
     8281 acagagcaaa cttaaattgt tcataggata actccttttc tgtattaact tctaataatt
     8341 tgatgtcttt ttataaaaca tatagataca attaaaccag taaatgcaag agtagctgct
     8401 attgaaaatg caacatctat tccatgaatg ttagacagca tgtttgaact catatgagct
     8461 ttcttagtag cacttgtcat tatagtaata agtatagcag aacctatagc tccagatatc
     8521 tgacggagtg tgttattaat tgctgtggca tgagatataa gttctctatc taatgtttta
     8581 attccccatg tagttaatgg cattaaaacc attgaaatac caatcatacg aatacaatac
     8641 atcaatgaca catgaattaa agatgtatct ctgcctaaaa aagcgaagga taaagttcct
     8701 aaaaacaaac atcctgttcc taatattgat agagcatgag gtccatattt atctaaatga
     8761 cgtcctgcaa ttggattgag tacaaccatc aatatagcac caggtaacat taatgaacct
     8821 gaagataatg cagaaaatcc tcttacagat tgaacgtaca gaggaatcat taatgtagca
     8881 gacatcattg aggcatatac aatcataatt aatattgtag aaataagaaa tggtttattt
     8941 ttaaaaaccc tcaattctaa aaaaggttct ttagatttta attgacgcaa tgtaaataaa
     9001 attaatgaca taattcctat taatattggt aaatatgtgg caatattagt ccacccataa
     9061 ttaccttgat tagtaaatcc aattaataaa ccaccaaaac ctagagatga tagtactatt
     9121 gaaggtatat ctaattttag tttttgagtt tccccaacat ttcttaataa aataaatgat
     9181 agtataacat ctaatatagc aataggggaa agaaacagaa ataaactatg ccaaccaaat
     9241 gaatctacta accatcctga taaagttgga cctattgctg gagcaaatcc tactgtaata
     9301 ccaactaaac tcatagctgc accacgtttt tcctcagggc ataaatagag tataataact
     9361 tgtaaaagtg gcattagaga accagaaccc attgcctgca acacacgagc aatcaacata
     9421 gttgaaaaat tattagaaaa aagtgcaata atacatccta tagaaaagat agacattgat
     9481 gtaataaata actttctagt actaaatcga tttattaaat aagctgttgt gggtacaata
     9541 atacctgcaa taagtagata aattgttgtt aaccactgac caacatcagc agtaatttta
     9601 aaatctgaca ttatattagg taatgctgtt gacaatagtg tttgactgag agaacttata
     9661 aaagcaccag tcattacaat tgctattatt aaatttcctt ttttattgtt actttgggtt
     9721 tccataattt ataacgtctc cttttttaat tgatttttaa tgctgttcaa taaaagtact
     9781 tttggtctta aagtgtcttc tttttctaaa tatgcaatca ttgcatccat aattaaagaa
     9841 tcatattgtt gattttttcc ctcgacgtat aattttttta ttgcttctag taatttagga
     9901 ttttcaagga gagaagtatc agttttgatt agttcttcta tatcttgtat cattttaaaa
     9961 aatagcgatt tattatgaag ggaaaagtaa acttcatctt cttgtttatg taaaaacatt
    10021 gatttttcaa ttaaaggatt tagattatcc atattttcta caactaaatc tagaatttgt
    10081 atagtaaatt tggcaataaa gtatacattt atttctcttt tttgtacatg aataatgtat
    10141 ataaactgat gaattagagc atccaataag aaaactaaat caaatataat tttttctatt
    10201 ttatcaccat agttatcaaa taagcttaat ttgtattcat tcaataagtt agctcgtgat
    10261 tttgtttgca atttacttgt agccttacgt tgctcttcag aaaaattttc tcttatatag
    10321 gtatttatgg tagttctaga aaaaatattt ttccatacag caattatctt tctgtttaac
    10381 ttttctgatg ctggaatgtc attattttca tttatggaat gtataatttc taaaaacttt
    10441 tcatcataat aattgattat ttcgcagata aaactttcct tagtttcaaa atgcttgtaa
    10501 aaagtagctt tagacatttt acactctttt acaacatccg atattgaagt attttgaaaa
    10561 cccttttggt caaaaatttt ttgtgcaata gttataatga gcctttgttt ttctgtcact
    10621 ttattatctc ctttcacgca aaaacgttta gtactataga gtacctacaa gttttaataa
    10681 atatattata catcatttgg aaaaaaaatc aatatataga ctgtaatatg aaatttttga
    10741 aaaatttgtt gttattttta gtcgtataaa aaagaaactt aagagtttct taagaattgt
    10801 ttaaagaagt tgcaataaat tagtggtatg attatagtat gaaagagaat gtcttgaaat
    10861 ttttctgtat caaattccaa ttatggaggt gtagtatatg aaaaagaaaa tttgtgtagc
    10921 aataattttt gctctattta tagtttttgt agaaatgttt attgatagct ctagcgaagg
    10981 aagtaaaatt atgacagctg aattaagtaa atacaggaaa ggcgtaaatg tattatcaat
    11041 aaacaatatt atagtagcga ataaaaaata tagtttgccc aaagattatt ctcctcagga
    11101 aagcagtgaa gcgagagatg ctttttataa aatgaataaa gatgctcaaa agtcaggatt
    11161 aaatttaaaa gcttttagta catatagaag ttatgaatat caagatagat tatttaagtc
    11221 atatgtaaaa gaacatggag aaaaagaagc aaatagattt tcagctaaac ctggagagag
    11281 tgagcatcaa actggacttg cttttgatat tggaggagat gaccagtctt gttgggcaaa
    11341 taaaaagttc aacaatacaa aagaagctaa gtggttgtat gaaaatgcat ataaatatgg
    11401 ttttatactt agatatccag aaggtaaaga gaatataaca ggatatatgt atgaatcttg
    11461 gcactataga tatgttggaa ctgaacatag caaaaacttt gcaatgaata atttgacttt
    11521 agaagaatat ttacatataa attaagagat ggtttgttaa aattaggtta aaataaaaaa
    11581 taacctagac tattagctgt gtaaagtgat ataatgtaaa tacaatttag ataactactc
    11641 gaaggggagt agcttacacg aaagtggtaa ttaatcgtca agacagtaga aatactcggt
    11701 taattagcaa gggattcctt gatagcaaga ccttcaaaat aagcattagg tatgtttgtt
    11761 ttggggtctt tttttattca aggaattttt atttaaaaaa atttaaattt caagtataca
    11821 taagagcccc caaacataaa ataaatttat tgaagaggag ggcaacaaat gtcttacaaa
    11881 agtacttatc aagaagtaat taaaaattta gactcaagtt tatctggttt gagtaaagaa
    11941 agagcagaag agttgttaga aaaaaatgga gctaatgaat taaaagaagc agataaggtt
    12001 cctacttata agcttttcct agaaagtttt aaagacccat tagtaataat tctattgata
    12061 gctgctttag tacaaatatt tttaggtgaa acagtggagt ctataataat ttttgcagtt
    12121 attattataa actctgttct gggtgttgta caaacaaaaa aagcagaaag ttcattagaa
    12181 agtttgaaaa atttatctgc accaaacgca aaggtaataa gagacaataa aaaaatgaca
    12241 attccagcaa aaaatctagt agttggagat attgtattct tagaagctgg cgattacata
    12301 ccagcagatg gaaggcttat agaagcacaa tctctcaagg ttgtagaagg gatgttaact
    12361 ggagaatcag aaccagtttt aaagcataca gaaaaaataa gtgaagatgt aagtttaggt
    12421 gaccagaaaa atatggtatt tagtggtgct gtagttgttt atggtagagg ttcttttgta
    12481 gtgactggaa caggtatgga tactgaaatg ggtaaaatag ctgggctttt agaaagtgct
    12541 gaaaataaac ttacaccatt gcaacaaaaa atagaggatt ttagtaaaaa acttggtgta
    12601 ggtattgtaa tacttgctct tttaatattt ataattcaag tagcaagaaa ttactttatg
    12661 gctggaaatg gagaaatgac acaaattatt ttagattcat tcatgttctc tgtagcagta
    12721 gcagtagcag ctataccaga agctctatca tcaatagtca ctatagtatt ggctgttgga
    12781 acaaatagta tggcaaaaaa acaagccata ataagaaaat taccagcagt agaaacttta
    12841 ggttcaacaa gtgtaatatg tactgataaa acaggtactc ttacacaaaa taaaatgaca
    12901 gtagttgatt attatatgta tggaactaat aatgatgagt tagataaaga aaaagtaaac
    12961 tattcttatc atgctaaatt attgactatg gtttctacat tgtgtaatga ctctcatata
    13021 actagtgatg gtaaagaagt tggtgaccca acagaagtag ctttaataaa ttattcaagt
    13081 aaaaatgatt tagattatga taaattaaga agtaagtata tcagaataaa tgaaatacca
    13141 tttgactcag acagaaagct tatgtctact gtaaatagta tatatggaga ttatataatg
    13201 tttacaaaag gtgctccaga tattgtgttt agtagatgta aatatgcttt gaaaaatggt
    13261 tcaaaagtag atataacaga tgagataata gaagaatata aacataaaaa tgaagagttt
    13321 tcaaatagag cacttagagt attagctttt gcaataaaag atgtcccaga agaagatttt
    13381 ataccttcaa ttgatgacga acatgacatg acattggttg gaataatggc aatgatagac
    13441 ccaccaagag aagaagtaat ggatgcagta aaagaggcaa agagtgctgg tataaaaact
    13501 gttatgataa ctggtgacca taaaacaaca gcagcagcaa ttgcaaaaga gattggtata
    13561 atggaagaag gagacttggc tcttactgga aaagagcttg atgctttgag tgaagaacaa
    13621 ctatatgaaa aactagaaaa tataagtgta tatgccagag tctcgccaga aaataaaatt
    13681 cgtatagtaa aagcatggca acataagaat aatataacag caatgactgg tgatggtgtt
    13741 aatgatgcac ctgcattaaa acaagcagat ataggaatcg gtatgggaag tggtactgat
    13801 gtagcaaaag atgcttctgc tatggtattg gttgatgata actttgcaag tatagtaaat
    13861 gctgttgaag ttggtagaac agtatacagt aatataataa aatcaattac ttatctattt
    13921 gcaggaaact taggagctat agtatccata gtatttgcag tgtttgcagg ttggtcaaat
    13981 ccatttacag ctttacaact tttgtttata aacttagtaa atgactcttt accagcaatt
    14041 gcattagggt ttgaaaagtc agaaaaaaat ataatgaata aacctcctag acacccggat
    14101 gaaagtatac tagcaggagg aacattgaag gcagttgcta taagaggttg tataataggt
    14161 gtagttacaa taattgcaca atatatagga ttacaagtat ctccagaact tggagtagca
    14221 atggcatttt caacattgat attagctaga ataatgcaaa cattacctgc acgttctaat
    14281 aatcaaacag taataaaatt aggttttttc agcaataaat acgttatagg agcagtttta
    14341 gtctgtcttg ggctttactc tttaacacta ataccagtgc taagaggaat attctctata
    14401 ccaacaacgt ttggattgta ccagttgggg atttgcctag ggttagcatt aatttcatca
    14461 atattaatgg aacttaacaa attaataaat agaagtgaag taaaataatc aaaaaattaa
    14521 agggcatatt gattttaaca atatgccttt tataaatagt ctaatttatt ctatattatc
    14581 gattatttgg aatgctttta taacttcagg taattctttt tcttcaggca ttctaaatct
    14641 tatgaaattt ttatcaagtc cagtaaaatc acttccagat ataactctta tttttaattt
    14701 agcaaactct tgttgtaaat ctatgtcctt gtttttatgt tcaactgtca taattgatac
    14761 agtatcacta gtttcagcta tattaaggtt tttccatgga attaataatt gattttttat
    14821 atcttttgtt ttttctttta attcttctat aaattgaaca tcatctaata aatttgctgc
    14881 aatactacga gatatttcag acacttcgta aggagtagaa atctttttaa tagggctaac
    14941 aagttgttct ggaagaactg catatcctgc tcttagtcct gctaaaccga atccttttga
    15001 aaatgttttt aaagcaatta cattatcata attatttaga agcttgacag ctgaattttc
    15061 ttttggcatg tattcaccat aagcttcatc taccataact gctatatcat atttagctgc
    15121 ttcctttact atattttcaa ttgatgataa atgtataatt tgtcctgtag gattgtttgg
    15181 attgtctatg taaattactt tatattctgg atttattttt tcaataaatt ctttttcatt
    15241 aaacttgaaa ttatcctctt tctttaaaag aacataatca taagttgcac catgcatttt
    15301 tatatctgtc tcatattctg agaactgagg tgaatatcct aaaactttat ctcccttttc
    15361 aataaatagt ctatttaaaa ggtaaataac atgtatagaa ccatctgcca aacaaattct
    15421 gtcagtatct aatgctataa aatctttcca ataatcgact atattatctt ttaaagcaat
    15481 tgaatgagga tatcctctta tcatatcaaa ttttaaagca ttaaaagctt ctacagcttt
    15541 gtttgagtaa gacacggtat ttataccttc accacagtct atctcgaata catcttctga
    15601 atgtccagat tctattgcat aacttttttt caactgcatt tcaatagctt tgttaatttt
    15661 tagtttcata ctttatcact ccttatttag tcaataataa atgttataaa agttgctaga
    15721 tttaaaatat tatgaaaata tataatagta aaaaatgaat ataaatatta taatagctca
    15781 ctttatttaa tatgattata tatcatatat tgtaaaattt ctagcattat aagactataa
    15841 attgaaaata ttcagaaaaa tactacaaga aacgatttat tttgctctta aaataagtta
    15901 aatgaatgtt attaattttg taaattgaaa tagtttaagt ataaagttat gatataatct
    15961 aataagggta attctttaga ttatattaag gaaatatagg tatacaataa aaagttatgg
    16021 agttggatta tgggatataa attaattgta acagatatgg atggaacttt attaggcaat
    16081 aatcataaag ttacagatga aaataaaact gcactacaaa aagttataaa aagcggtatt
    16141 aatgtaactt tagcaacagg aagagccttc gattcagcta aatgcaatgt agattttctt
    16201 aaagaggaca tgccaataat agcttgtaat ggttcattaa taagagagca aaatggaaat
    16261 ataatatatt caaataagat tgatacaaga acatgtttaa acattttaga tgtattagat
    16321 aaatacgata tttactacca atgtaatagt atagattcta tgctttcgaa aaaaatagaa
    16381 ggacgtgaag atagattaag tgtattttta ggctctgaaa cagaagtaat tgtaaaagat
    16441 gatttgagag aagaaatctt taaaaaagat atactaaaat ttgtaatcat agaagaaaaa
    16501 aatccatcta ttttagatga aataagaaaa gaattgagaa aagttcaagg aataaagata
    16561 acttcttcat ggccaaacaa tattgaagtc atgaatgaag gtgttgataa aggtaatgca
    16621 gttaaaattt tagctgaaaa aatgaatata gacagagaag atataattgc atttggagac
    16681 aattacaatg acatagaaat gataaagttt gctggtcttg gggtagctat gggaaatgca
    16741 gaagaattga ttaaacaaga agctgattat gttacagata caaatcaaga cagtggagtt
    16801 gcaaaggcaa tttataaatt cctagagtta aaagaatcac ttagctctta aaacagtata
    16861 taataggaaa aaataggttg actattttgt agatagtaat tataatacaa atagataaaa
    16921 cataaaaatt tagattcagt gaaaaagaga gtagatatag agaaaatctc agcgaattgg
    16981 gggatggtgt gagcctaatg ataacacttt atattgaaaa gaactttgga gaatactact
    17041 tgaaattcag tatggtagta ggtaatctcg ccttaagaga atagagtggg taaacattgt
    17101 ttaccaacta gagtggtaac acggaatatt tcgtctcttg gtctttttga ccaagagatt
    17161 ttttagtttc attgagttac aagacttata attaaaatac aaaaaattaa aatatgaggt
    17221 gaaagttatg aaattatggg gcggacgctt tagaaaagcg gaaaatcaac ttatggaaga
    17281 atttaataaa tcttttggct atgattgtgt actgtacaaa aaagatatcg aaggaagtgt
    17341 agcacatgtt catatgcaag taaaatgtgg acttttaaca gaagaagaag gaaagtctat
    17401 aacagaaggt ttaaaaggga tacttgaaga tgtagaaaat ggaaagttag tattggatgg
    17461 agagtatgaa gatatacata gttttactga gataaattta attcaaagaa ttggagatgt
    17521 tggcaagaag cttcatacag cacgtagtag aaatgaccaa gttgcagttg atatgagatt
    17581 gtatgctaaa gaaaaagcaa atgatttggt gggtttaata tctgaattta aagctactat
    17641 aaaagatgtt gctgacaaaa acccagtgat gatgccaggc tatactcatc ttcaaagagc
    17701 tcaagtagtg acatttaagc atcacttaat ggcatattat agtatgtttg atagagatga
    17761 gaaaagaata aaaaatgcta tagaaatatt agatgaatca cctttaggct gtggagcttt
    17821 agcaggaact acgcatgata tagatagaag tataacttgt gaacaattag gatttaaaaa
    17881 agtagtagat aattttatgg atggagtaag tgatagagat tatttactag agctaatgag
    17941 tgatttttca attataatga tgcacttaag tagattaagt gaagagttga tactttggag
    18001 ttctcaagaa tttggatttg ttgagataga tgatttatat acaactggaa gtagtataat
    18061 gcctcagaag aaaaatccag atggtgcaga gcttataaga ggtaagactg gcagagtata
    18121 tggaaactta tttggtcttt ttacagtaat gaaaggaata ccattagcat ataataaaga
    18181 catgcaagaa gataaagaag gttttttcga tagtgtacat actcttgaaa tgtgtataca
    18241 aataatggat agaatgatag ccacactaaa ggtaaatgag gataaaatga aacaagcagt
    18301 aaaaaatggt tttttaaatg caacagaagt tgcggattat ttggttaaaa ataatgttgc
    18361 ttttagagat gcacatggta tagtaggaag tatcgttata tattgtgaag ataataaaaa
    18421 agcaatagaa gatttaacat tagaagaatt acataagttt tcagatgctt ttaaggaaga
    18481 tatttatgat tttatcgact atgagagtat attaaataag ggaataaaaa agaatttaaa
    18541 ataatgtatt tatttttaaa agctgtaaat ttgaatgtta tcatttaaat t
//
LOCUS       DAMKMI010000076        18573 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMKMI010000076 DAMKMI010000000
VERSION     DAMKMI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.077"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            356..1207
                     /locus_tag="PC429_002325"
     CDS             356..1207
                     /locus_tag="PC429_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF3847826.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1211..1681
                     /locus_tag="PC429_002326"
     CDS             1211..1681
                     /locus_tag="PC429_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847827.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1694..3004
                     /locus_tag="PC429_002327"
     CDS             1694..3004
                     /locus_tag="PC429_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3847828.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3216..3962
                     /locus_tag="PC429_002328"
     CDS             3216..3962
                     /locus_tag="PC429_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3847829.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4324..5865
                     /locus_tag="PC429_002329"
     CDS             4324..5865
                     /locus_tag="PC429_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF3847830.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5900..7006
                     /locus_tag="PC429_002330"
     CDS             5900..7006
                     /locus_tag="PC429_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF3847831.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(7337..8623)
                     /locus_tag="PC429_002331"
     CDS             complement(7337..8623)
                     /locus_tag="PC429_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3847832.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(8746..11844)
                     /locus_tag="PC429_002332"
     CDS             complement(8746..11844)
                     /locus_tag="PC429_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF3847833.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSSDFEKLLY
                     QK"
     gene            12569..12721
                     /locus_tag="PC429_002333"
     CDS             12569..12721
                     /locus_tag="PC429_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847834.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(13014..13718)
                     /locus_tag="PC429_002334"
     CDS             complement(13014..13718)
                     /locus_tag="PC429_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847835.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(13709..15316)
                     /locus_tag="PC429_002335"
     CDS             complement(13709..15316)
                     /locus_tag="PC429_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF3847836.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(15570..16748)
                     /locus_tag="PC429_002336"
     CDS             complement(15570..16748)
                     /locus_tag="PC429_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847837.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(16974..17468)
                     /locus_tag="PC429_002337"
     CDS             complement(16974..17468)
                     /locus_tag="PC429_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF3847838.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(17469..18230)
                     /locus_tag="PC429_002338"
     CDS             complement(17469..18230)
                     /locus_tag="PC429_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF3847839.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         6333 a         3014 c         2363 g         6863 t
ORIGIN      
        1 tacttccact catttatttt aaaatttcaa tttactattt ctcaattcta tattattatt
       61 tttcaatttt atattactaa ttttaaagct ttttagctat tattcctaca atatttaaac
      121 aattttttaa taataaagct atattacaat atattcaaaa tatgtaatat tttagatatt
      181 tttcgttgac aatgttaaat ttttgtttta tccttaatta aggacatgtt attgacattt
      241 ctgaaactta tttgaaatag ttttttcatt ttatttagga aaacgtatta tacctaagaa
      301 aacgtatttt ctcatttcag ataagtaaaa tatttattct tttggggagg aaatcatgga
      361 aattaataaa aaacatttta ttcaagtatt tttaacttgc ttaatcttgg tagtaatatg
      421 tgaatttata gggcaaagaa ctatagctat tggaaaagcc tctatagtac tgttccctat
      481 gctttatgca gttataattg gacttataat aacaccagat ttattaggta aaaaaattaa
      541 agcacttaaa aaagtggttg gagaaaaaga aataaatatt gctggtgaag ttgttggaat
      601 tgcactagta atgcttggta taaaatatgg tactacagtt ggaccaaaca ttgacaaaat
      661 aattcaagct ggaccagcat ttatcgctca agaatttggt catatacttg caccaatcgt
      721 tgcattacct cttgcattaa tgtttggaat gaaaagagaa gctgttggag ctactgcaag
      781 tataagtcgt gaaccttctt taggagttat atcagaaaaa tatgggataa actcaccaga
      841 aggtagtggt gtacttggta catatttaat gggaacagtt cttggtacta tatactttag
      901 tgtactaggt tcaatatcta tttatagtgg tcttcaccca tatgctttgg gaatggcctg
      961 tggtgttggt agtggaagta tgatgacagc agctgctggt tcattatcta ccatggtacc
     1021 acctgaaatg gcagatacaa tacttgccta tgctgcaaca agcaatatga tgtctagtat
     1081 aacaggaatt atattcttag tttttgtatc cttaccattt actaatttta tgtataagat
     1141 tttagaacct aaattttcga gaaataaaaa aactaaagaa attaaatcta cagaaggaga
     1201 gtgctaataa atgtttgcta atttaaattt aaagaaaagt tcatatcaat tattggttat
     1261 gtctgttatt ggagttataa ttcttatagg acaaagaatt agcgttggta catctatagt
     1321 tacagctctt cctggtatgg tgatgctaat attagctgct atggcagcta tgattataaa
     1381 agatttattt cctaaatcaa tctttccagc ttttggattt gcaactataa tcggcctttt
     1441 acttagtatg ccatatagtc caacatctga agtattctta actaacacta ataatattaa
     1501 ctttatggct ataactactc cactactagc ttttgcagga atttctgttg gaaacaaaat
     1561 agaagcttta aaagaaatgt catggaaaat agttataatt tcacttattg tatttacaac
     1621 tatattcttt gcatgtgcga gtatagctca tatagtttta agtattcaag gtaaaatata
     1681 ggaggaattt attatgaata aagaacaatt aaaatctcta gtaataaaaa ctatagatga
     1741 aaatagagaa aaaatattaa aaataggtca aggaatatac aaaaatccag aatatggata
     1801 taaagaattt aaaactacag aagctgtttc aaactttttt gagcaagaat taaatttatc
     1861 tgtagaaaaa aatatagcag ttacaggatg caaagcagat gcaaattcta gcaagaaagg
     1921 tcctcatata tctatacttg gagaattgga tggtatatca tgtaaagaac ataaagatgc
     1981 taatgaaata ggtgcttctc atacttgtgg acataatatt caaatagctg gaatgttagg
     2041 tgctgctgtt ggtttagtaa aaagtggcgt tttagaacat ttagatggta aagtatcttt
     2101 tatggctact cctgctgaag agtttattga gttggaatac agagagcaat taaaaaaaga
     2161 aggaaaaata acttattttg gtggaaaaca agaattaata aaaagaggtt attttgatga
     2221 tatagacata tctatgatgt ttcatgcttc tgatttagga gaagataagg ctctagttgg
     2281 tcctgagagt aatggatttg ttggaaaaaa ggttaaattt ataggtaaag aatctcatgc
     2341 tggttctgct ccacatgatg gaataaatgc tttaaatgca gcaatgcttg ctataaacaa
     2401 cgtgaatgca cttagagaaa cttttaaaga gtctgaaaga gttagattcc accctatcat
     2461 cacaaaaggt ggagatatag taaatgttgt tcctgctgat gttcatatgg aatcatatgt
     2521 tagagctaga actataaatg gtatgattga tgctaatgaa agaataaata aggctttgat
     2581 ggctggtgga atggcagttg gagctgatgt tgagataact gaaattcctg gatatcttcc
     2641 aatattacga tacaaaagtc ttgataattt atttaaaaac aatcttgaag atttaggtct
     2701 aaaaggaaaa gttattgatg gtggagattt tacaggctct tttgattttg gagatgtgtc
     2761 tcatataatg cctactcttc accctatgat tggtggtgta aaaggagctc ttcatacaag
     2821 agattttgaa atagttgatg aagatttagc ttacataatt cctgctaaag caatggcact
     2881 tacagttgta gaccttcttt ttgataatgc taaggatgct aaagatatat tagatacctt
     2941 tactccagtt atgactaaag aagaatatct aacattctta gaaaaacacg ataaaacttt
     3001 ataagtctat ttttataata gggtggtatc tactgtaaaa gttataccac tctttgtttt
     3061 tgtaacagta tacttctttt cttttagttt ttagattaaa ctaagatata ttatcctcct
     3121 taaaactttt agagctacgt tgaaatatca gtagaactat gctatattct ttctaattaa
     3181 tataaatttc tattctaatt caaggtggtg aaaaaatgtt taatgcagga aatcgaatta
     3241 taaatacatg gttgtaccct attcaagatg gctttgtctt gattgatact ggatatgaaa
     3301 atggttttaa acatttcaaa aaacgaattt ctaaattcaa cataaaaatc aaagatattc
     3361 gatacatatt tctaactcat gctcatgatg accatgttgg ttttctaaat cagattctag
     3421 acgaatctcc taatacaaag attgttatgc atgataaagc tttagaaatt ctatataaag
     3481 gtcaaaactc ttttattggt ggttgtacca gccgtactgc ctttctcttt tgccaaatta
     3541 tgaaactatt tggaaaaggt gagcatagtt tttctccact taaacaagaa tttgaaaaaa
     3601 ggtgtattct tgtgtctgac aagaatcgta aagaaattga aaaagaacta aatggaaaaa
     3661 ttatagatac accaggacat actgctgatt caatttcact actaattgat gaaggtgttt
     3721 tattttgtgg agattctgca atgaatggat ttccaagtac tcacaaaatt accatatttg
     3781 cagaaagcaa actagaattt attcaatctt ggaaaacaat catcaataca aaacctaaaa
     3841 tgatttatcc tggacatgga aaaccatttg aatacagtaa attagaacaa aatctaatct
     3901 atgtaaataa aataaattta tatcctctac ataattcaaa agatattcac aggtttttgt
     3961 agaaaataca aagtaaattt atttgattaa ttaaaaaatc taacacttta ctacgctact
     4021 tattttaata acttaatctt ccactagctt ttcataaaat tttgttttaa aattaatttt
     4081 aaagcgcata tgttatttaa atacattaga taaaattcgt cattaatcta cttaaagcat
     4141 atttaactaa caccactcaa ccagaaaatt cagaaaactg cattgacaca ggaaaaagtt
     4201 tattttatac tttttttagg acatattgtg gacacgtctt aaatcgacaa acagaattta
     4261 aattgtaaga ttaaccacaa tacttatttt ttaattatat ttttaaacat tagggaggat
     4321 attatgaatg ataacgtagt taagaagaaa aacttttttg acaggacatt aaacagaata
     4381 gaaactgttg gtaataaact accagaccca gttacaatat ttttagggtt atgtgtactt
     4441 ttacttatac tatctagtct agttggttct atgggtatat cagtagttca tcctggtact
     4501 ggagaaacta taaccgctgt aaatttattg actgtagaac agcttcaaat tcttttagga
     4561 aacatagtca gcaatttcca agggtttgct cctttgggtc tagttctagt aactatgata
     4621 ggtgctggtg tatgtgacaa aacaggactt atgacagcaa caatcaaagc atcagtaagc
     4681 aagattcctg aaacaagagt aactcttgtt gtaatgacta taggtatgct tgccaatata
     4741 gcttcagatg caggtacaat attatttcca ccattagctg cacttgtata tcttggtgtt
     4801 ggtagacatc cacttatagg tttattttct ggatatgcag cagtctgtct aggatttgcc
     4861 gcaaacatta tgataagtgt caatgatata cttgctgcaa gttttacagt tccagcagct
     4921 caaatgattg attctggatt tcaagccaat gcaacaatga acttattttt tatgatagca
     4981 tcaacttttg ttctaatact tttggcaaca tgggtaacag aaaaaattgt agctcctaga
     5041 tttggtaaat atgaaggaaa tgcagaacta gatgttgatg gaagtcttag tgatgtagaa
     5101 aagaaagggc ttaaaaaagc tggtataagt atattaatat atgtggctat tatagtagct
     5161 cttagtgtaa tcggtaaaaa accattctta gctgacccag aaacaggaga tttactatca
     5221 aacaatgctc ctcttatgaa aggtatggta cctataatag cactagcatt tttcatacct
     5281 ggtgttgtat acggaaaaac tataggtaaa ataaagaagg ataaggatgt agtctctata
     5341 atggctagtg caatgagtga tatgggtgga tatataatac tagcatttgc tgcttcacaa
     5401 tttttgcaat tattcacaaa tagtaattta ggtctgatac tagctgtaaa gggcggagaa
     5461 tttttaaaat cagcaggaat aaatggacct ttattgttga taggatttat tatattatca
     5521 tgttttataa atttatttat aggtagtgca tctgcaaagt gggctattct tgcacctata
     5581 tttgtaccaa tgtttatgat ggttggatac aatccagcac ttactcagat ggcttatcgt
     5641 ataggtgatt gtataacaaa tccacttagc ccattatttc cgtacttccc tattatacta
     5701 gcattcacaa gaaaatacga taaagatgct ggtatgggta ctgtaattgc taatatgata
     5761 ccatactcta tatcattctt aatagtgtgg actatactat taatattgtt tatggtattt
     5821 aatattccac taggacctgg aatacttcct tcatatagca tgtaatttat ttaaatcttt
     5881 ctaaaaggag aaataattta tgttagataa aaatagactt ataaataatt ttatggatat
     5941 ggttagaatt gatagcccat ctaatcaaga attagaaatg tctaagtggt tggtaaacta
     6001 cttaaaagaa agaaatatag acgccattat agatgatgca ggtgaaaaat atggtggtaa
     6061 tacaggtaat gtcattgcct atataaaagg tgaagaagga agtagaccac tttgtttatg
     6121 tgcacacatg gaccaagttc aaccttgttt aggtgtaaaa ccaatactag atggtaatgt
     6181 agttagaagt gatggaacta ctacacttgg tgcagatgat aaagctggaa tagctgctat
     6241 acttgaagct ttagaacatg ttattacaga aaaaatacca catagagata tttatctttg
     6301 ttttacaatc tgtgaggaag ccggtatgca tggtgtcaaa aactttaatc ctgacaactt
     6361 gccttgtaaa gacatggtga ttttagattc tggtggagct attggttcga tagcatacaa
     6421 agctcctgct caacaatcta taaaaatctc ttttcatgga aagaaagcac atgctggtat
     6481 agaacctgaa aaagggttaa atgctatatt agtagcctct catgcaataa gcaacatgca
     6541 cataggcaga atagattcat tgactacttc aaatataggt aaaatagaag gtggtggagc
     6601 tacaaacata gtaactgata aggttacatt gactgctgaa attaggtctc atatacctga
     6661 aactttagag tatgagctta atcatatgga aaaatgttgt aaggatgctg catctaaatt
     6721 taatactact tatacttttg aacatagtat gtcttaccct agttttgaac ttagtagaga
     6781 tagtcatgtt ttcaagttaa gtgaagaagc aataagacaa gttggtgttg ttccaaatcc
     6841 aatggtaatt ggtggtggaa gtgacgcaaa tatattagct aatcttggat ataattgtgc
     6901 tatattaagt ctaggtatgt atgatgtaca cacagttaat gaatatgtaa atatagacga
     6961 attatatgat actgccaaaa tagtttatca tatgattaaa ttataaatat cataatataa
     7021 ctctttaaat tataaaaata aaaacctatg tatcttcaac tttataagtt atatattaaa
     7081 gttgttttac ataggtttta gtattttata taataatttg ttttaacatt tttcataata
     7141 agcttcaata ttttgcataa taatttgttc tagcattttt aataacaagc tttagtattt
     7201 tatattaata atttattcta gtattttaca taatcatttg ttctaatttt tttcataata
     7261 agctttaata ctttatatag taatttgttg tagaatttta tacaatataa tgatttatac
     7321 agttttataa aatatcttaa aacagtattt cagtaatatt ttttggtctt cctccaccaa
     7381 tactagtctc ttttgcacat attcgaccta aacctgcatt tactatttta tttaatatac
     7441 gtcttgcact tctttcactt acatttagat actctgctaa ctcttttgaa tcataaatct
     7501 tactttttct attctcattt atagcaataa tttttgcaac tgactcacaa ctcattccag
     7561 tctttttaga tatttcgatt accttttcat cagatagcat caaagaataa tttaattcat
     7621 tttcggaagc taaaggtcct cttatgtttt catcttcatc taccaaatat ataccaattt
     7681 cttttgaatc tatggagtgt cttaatgctt tatgtgcatt attttcagcc tgatacgcag
     7741 ttactccagt accaattcct atactaagag aaaagcccat gtcttttatc tctctcttta
     7801 gattgaatag cttatcataa ttatactcat tatcagctac ccctttatga acaaatacaa
     7861 cataatcatt tcttccaagt aaaaataagg aaccttgaat acttcttaca tagtttacta
     7921 caagcttatc catatctgat ttttttatca tgtcagaata atagttatct atcttctctt
     7981 tataatctat taaactcaat atttcaacag caatttgaga aaatcttgcc ttacttagag
     8041 catattcact ctttaccttg tcataacttt ctttgattaa ttgtatagta gcttgtaaac
     8101 gaaatacagg atagcctctt tcttttaatt cattatatac tgcaccaaaa ccagttaaca
     8161 ttatatccgt cttattttct tcaaataatt ttatatgcca ttctacaagt tcagactctc
     8221 ctacttccaa agaaaatgga ttacaaaata cactcgttgc tttaatatca aattctttaa
     8281 gtgcatcttc cacaacatat ctatcaacta catctatact gaatttatct aattttatat
     8341 ttgcactttt tattgcccaa aatgccttca ttatactcgt cccacttctc ggtacaaatg
     8401 aatgtggctt acttatctca tacttagatt ttataaattc ataaactgca catcctgtaa
     8461 atataatagc atcacattcg tcttcacatt tagaaataac ttctattgta tctactactt
     8521 tttctcttac atatatttta gtctttaaat cactgtctat ctgttttaaa tcatccttta
     8581 tcttaaaccc agagtctgaa ggacctataa tacccactat cataaacttc gctcctaatc
     8641 cataaatatt atacttttta aaaattatat cctcactatt ttctacttct atttaattat
     8701 tttgaactac tccatcctac ctacatttag aatggaataa ttagcttatt tttgataaag
     8761 taacttctca aaatcagaac ttggaatagg cttacaataa taataacctt gaattaccac
     8821 acaatctaaa ctcactaaaa attcagcttg ttcttttgtc tctacacctt ctgccactac
     8881 atccatatct aaatcttttg ccatggctat agtgtgcttt aaaactattt tttctctgtc
     8941 tgtgattcct tcctcattaa agaaaccaca atctatcttt aatgtatcaa tattaatatt
     9001 ttttaacata tttaatgaag aatatgctga accaaagtca tccatattta aaacaatacc
     9061 atccattcta aggtcggcta ttatcttagt aaataatttt atatttttaa taaataaact
     9121 ttctgtaagc tctaattcta aaaaagattt tggtatacta tatttattta ttaaacttaa
     9181 aacaatttct ttaaaatgtg gattatgcaa atgtaatctt gatacattta cagagatagg
     9241 aactggctct tcacctaaat ctatccattt tctaatcaaa gaacatactt gttcccatac
     9301 atacatatct aatctgataa taaacccatt cttctcaaat agtgaaataa atttaattgg
     9361 aggtattata cctttttctg gatgaaccca tctaaccaat gcttctgcac caacaatttt
     9421 atttgcatta agattatact taggttgtaa atatacatgg aattgcccat tttcaagtgc
     9481 gtattccatt tccctttcca tctgtttttc ttcaagtgca tcatttctta tagtatcatc
     9541 ataaaatata tagttgttga tactttgttt ctttactttt ttgtgagcat atcctgccat
     9601 ttcacacgca tttgtaattg gaatatgatt atccaaaatt ctataaaccc caaaacatgg
     9661 tactaacata tattcaaatt tttttctatt taagctattt ttaatcttat aaattacttt
     9721 atgaatatct ctatcagagt aactctcata aaaaatgcaa aatacatctg catacatacg
     9781 acaacatata ccattagaac caatgttttt ttgaatcaac tctgcaacat atattaaagc
     9841 tttatctcct cctctaactc cataaatgtc attaattacc ttgaacttat ctatatccat
     9901 cattataatt gcataacgct tgtcaggata tcttttaagc attaaactta tttgttcaca
     9961 aaaactttca taattgtata gtcctgttaa tgtatctttt tggtttttat gtagttgcat
    10021 aagataatct atatttttta tagcaactaa aatatgtgtt tgtttctcac ctaacttatt
    10081 tttactacta atctttaaca tataaattaa tgcccaatag tactttttat ctattcctaa
    10141 acgtctatac tcaaaattta gttctaaatc gtcttgcttg ctattattaa gactatcaag
    10201 agattttaga aggtggttat cattaaataa tattatatca tcaggatgaa ttaaatattt
    10261 ttttatgtat gaaaaagttt tttcaagatt ttcctttata ggaaaactaa aaatattgct
    10321 ctctgattga taaattggag taattaaatt cgtatcaatg caaatagata aaattatatc
    10381 atatgtatta taagctaatt tttcataagc tttagaatac ccttttatca ttaaatcatc
    10441 aaaataaacc ttatcaacta actctttatg tttaaataaa atattattcg tcgaatcagc
    10501 taagtcacta attactttca tttcattatt tctaagtttg ttcttattat aatacctctt
    10561 cttatcttgg tacattctct catcagcaac tttaattagc ttatgtatat caatattatc
    10621 gtcactccaa atactgccca ctgctacact atatatcatc tcatccttaa attcttcact
    10681 taaccaactc actaattctt caaatagttg ttttgatata tcttcacata atacaacaaa
    10741 ctcatctcct ccaatacggt aaacattata agaatgaaag tattttttta ttattacaca
    10801 tgctgacaca atagcagaat cgccattagc atgtccaaag ttatcattaa tatcttttaa
    10861 cccatttata tctacaaata caactcctat ggatttaact tttccttttt gaaaattatc
    10921 tatatactta atatatttat ttctattata tgcaccagtc aagaagtcat agtagctcat
    10981 ataatttaat ttattttcta cttttctttt tttcatctca gttaataaaa aataagatag
    11041 ggatgtcaaa agagaggtat ctccaacagc tttatgtgga ttatcaactc caataaaccc
    11101 tactacttta tcatcttgtt ttagtgaaga tgtcattaaa ctttcaattt tttgaggttt
    11161 taaaatttca tattctattg gctgttcttc ttttatattt tctatatcat caattattac
    11221 aaaacctttt tgttcgaata ataaaaacca attctttaat tcattaatat caagatttgt
    11281 tagtgcctgt ttttgttcat caacaccttc attgcaccat tcatatgtat tagttacttt
    11341 tgttctatct tctgatactt caaaaacata tgctctatca gctttatgaa atttaccaat
    11401 attggttaat acaagattta cagcatcaga aaaattagat tctgttataa gatgtttaat
    11461 acatccaact aataactcct ttatctctaa tttttctgct attgacttac ttatatcttc
    11521 cttttctgta atatcaagag ctatttctaa tcgtgcatgt ttgccctccc atttaattaa
    11581 cttgtccttt aatataaaat gtctatttgt tcgagtattc gtattttccc atatgtaata
    11641 ttcatcttct tttaaaatag agtttgtaca aaattcgcag ggagaatctt tcccctgtaa
    11701 tgctttataa cattttaaat gggatatatc attaagtccc attgcctttt tcccaatttc
    11761 attaatatat aaaagttcat atgtatctaa agaagaaaca tatacaattt catccaactc
    11821 atctattaaa tttattacat ccatttatat atatctcctt ttattcattt tccagattga
    11881 atttttaaag cttttaaaat tttgctatat atttggcaat gatattatac ttctttatgt
    11941 tttaaaatag taccttaatg aggttctatt ttttagtaaa tagaattatt attgtgtcaa
    12001 tcgtacaata gagttttgta atacttaatt taatatattt ataattaact atcttattat
    12061 gtatttacaa ttaattctca tataaatgac aaatctccac ctcaattaag tattccaatc
    12121 atatatgagg ataatacttc attcatttaa aaatataaaa ctattatata ttaaaatttc
    12181 ttcaataata aattatttag attgttactt tgatatatta tataatatca caaaggctga
    12241 tataaaaaga caaaaagggc aattttttta tatcaaaaaa aatgttaaat tgatattatc
    12301 catatataaa ttgatattat ccatataggt gctatattta acaattctac ttacttccct
    12361 tacacttatt agcatatagt tattaagagt ttgttgcaaa acaacactta gcacacttta
    12421 aaaagtgtgg tctaataagt aatcaacgaa gaagacataa taaagcataa aataggatat
    12481 acttttccac gtcatcaaaa ttgaggattt tattattaat atatgataaa atatgcttaa
    12541 taaattaaat tttaatggga gaatagttat gaaaatattt agtttcttgt tgtttgtagt
    12601 gggaatatgt ttttgtattg ctggatttat ggggcattcc tcagcttttt ctggtgcaac
    12661 attgagtttt tttgcaacta caatgatagc tgtaagacaa gtcaaaacta agaaaaaatg
    12721 aaataggcaa atcctaactt tataagttaa tattacataa gtaattagtc taaaattaga
    12781 tactttttct attacaaaaa tacatcatcc actttgtatt ctcttaaaaa aaataaaata
    12841 gctatgtaaa ttcaagatta gatagactaa ctctctatcc atttcattgt aaaatataca
    12901 tagctatttg tattcgattt aaaatactta gattttctta cataacaaat aatttctatc
    12961 tgattattac tcatgttcat ttattaacca ctcagcaact aatcatttag tatctattca
    13021 tccgccatac ggtaacctac cccaacctca gtcaaaatat attctggctg agcaggattc
    13081 ttttcaattt tccttcttat acttgccata aaaactctaa gcgactgatt ttcactttca
    13141 tagtaatttc cccaaacttt atctattata aacttatggg ttaagactct tcctgaatac
    13201 ttagacatca actcaataat cttatattcg attggagtta aatgtatttc ttcaccatta
    13261 attgacacaa tatgattttc ataatctatc tttaggtttt taacttcaaa agactttgga
    13321 gcatcattat ttatattatc ctgttgagct ttatttctaa gagccacacg tactctagcc
    13381 aaaagctctg ttacactaaa aggtttagtt aaataatcgt ctgctcctcc atctaaagct
    13441 tccaccttat ctctctcatg ctctctcgct gaaacaacaa ttattttatt agtctttgca
    13501 catgccctaa ctcgtcctat tacctcaatc ccatcaatat ctggtagtcc taaatccaaa
    13561 attatcaaat ctgggtcaag agaatgtata agtgatatag ctgttcctcc attatctgtc
    13621 tctacacacc tataattttg agtttcaaga gatactttta taaatttaca aattggctta
    13681 tcatcttcta caattagaac taatggcttt atttccactt gtctttcccc tcctcatcat
    13741 acataggtat gctaaatcta aatgtagctc ctccagaagg attattaaag gcgacaattt
    13801 ctccaccatg agcttctact atagatttac atatctccaa tccaagacca atacctcttc
    13861 tttcaagatt tctagactta ttcttagtat aaaacctatt aaaaatattc ccgatatctt
    13921 catttggtat tccttttcca ttatctgcaa cttcaaatag tacttgattt gactcttttt
    13981 ttacagttag ttctatctta gaatttttag gtgtatgcct tatagcatta tcaattagat
    14041 ttactaacac ttgttctatt aataatacat ccacatgaac gattattatc tcatctggaa
    14101 tatttgtttt tatgtctcta ctattggcaa actttttgac tcttaatata gattcagcta
    14161 ttatttcatc aacaacctct ggtcgttttt caatatccaa ttttccttca tctattctgg
    14221 tcatactaag tatattctca acagaatgag ttagccaact cgtatcttca tatatatttt
    14281 tcaataattc tactctagtt tctttatcta tcacatcatc attttcaagt attgttgaag
    14341 atgaacccaa tatacttgta agtggagttc ttaaatcatg agaaattgac ctaagcaagt
    14401 ttcctcttaa cctctctctc tctgcctcaa ggtttgcttt tttatttttc tcaaataaat
    14461 tttctctatc aattgccaac gcaacctgtg ttgaaactgc ttctaatagt atcttttcat
    14521 tttctgtaat aatatctatc ttaaaacacg caacacctat aacaccaaga acatttgatt
    14581 cttgaccttt tatagggaaa taatatgcac tgctgttttt acaatatttt gtcccaattc
    14641 caactgggtt acctgtccta aaagcttctt ttatagcttc cttttctaat actgaatgga
    14701 atatattctc acttccatca ttgttacaaa tataaatact tggcttacct agattattat
    14761 tctcatctgc aatagtcact attacagttc tatttaatat atttactaaa ttagacccac
    14821 aaaattcaat aatttgattt ttattttttg ctttaagcaa cgttttgtta ttctcataga
    14881 gcaatcttat cattttttct ctcttatgag aaagtctcac ttctcttttt atcctagaag
    14941 ttaaagtact tgttataatt gctgccatta gcataataca aaaggtcata ggataatctt
    15001 ttctataagc taaaaaagta tataatggat gcgtaaagaa aaaattaaat gatagcactc
    15061 ctattactga agaaagtatt ccaaaaaaat atccataggt tatacttgta gcaaatagta
    15121 cacctaatat gtatactagt ataatattag attcattaaa acctaccttt tcaaaaacta
    15181 ctgatattcc agtagcaatt gtcataacca atatagtttt tatgaagtcc ttaataatta
    15241 tctttttact gagatagcca tcttcatcat aatcatactc ttttaagtct tcatgttgta
    15301 gtcgttcctt tttcatttac tacaattcct ccttattgtt actgtttaaa ttggctctat
    15361 tttatataat acaattttat cataataaat tcaatcaata catttaatta atgtacttaa
    15421 attactaaaa ggtgctgtct taaaatgaaa ataaatctct atttcaaaac agcacctata
    15481 tatttaaatt actaatttag tttttacata cttaaatcaa tagttttatt caatttcata
    15541 aattataata cttcaattat aaaacatttc tatccaacaa gcataaatat aaagtgggca
    15601 catatacctg cacataaacc acctatagta tgcgaaagta tcgctttacc agccaatggt
    15661 cttgcatcta aagcatccat catacctatg tgtgtactta aatatccact ccaacacata
    15721 cccattgctg tgaatactgc tatatcatta ggagttatag cgcctgattt tataaactct
    15781 ggtactaaag atattgctgc tccaacagct cctaatgcag ttacagggaa tgctattgcc
    15841 tctggtgatg tgaatccaaa taatggttct attatgaagc taatcttatc accaatagca
    15901 ggtaataatt ttataccttc atatgcagct cctgtgtaaa cagcttgacc tgtagcaggg
    15961 tctgtagatg gtccaaaagt taataacata actaatgtac aaactactaa aactcctggt
    16021 attatagcca tacccatttc aacacctaac ttaccacctt caagtatagc atccaatgtt
    16081 ctttggaata cattaccatc ccttatttct ctaaactctt caagttttgc acctttatca
    16141 tttatttgct tttcagtatc atttttaggg tcataattat aatacttttt agtaaaagtc
    16201 aacataattc taacactaat aatactacct attattgccc caacattacc tattatagct
    16261 ggtagtacat attctttacc ttgagctatc ataaaagttg ttacaataag tcccatacca
    16321 aaggctgttc ctaaattaca taaagcaggt acttgatact tcttaaaata ttgtgtaaat
    16381 gttttgtctt tagcaaatgg tataatagct ggattatcag ataaatatgt tgcaactact
    16441 cctgctatac tagcaccagg taagccccat ataggtctca taagaccctt aaatatctta
    16501 ttaactaaag atattactcc aaactctgaa agcaatgcac taagtgcacc tgcaagaact
    16561 gccatagcca ttattaaaaa tactgtgtct aataaaagcg catgtgctgt actcattatt
    16621 actttaaaca tcattcctgc acccatgatg cttcctacat atccaaatcc taccgccaat
    16681 agtacaataa atacgaaggt ttccatactt actgccttga ctcttctctt atccgacagc
    16741 ttttccatct tgaaatcttc ccccttttaa ttttaaatta aggttcctaa cattggtaca
    16801 aattttttgg tacaaactga gttctaaaac ttacactcgt aatgcaagtt gtcagttttt
    16861 tgaaataaaa aaaaattacg tttatagaat aagaatatcc tctttaattt ggatactctt
    16921 attctatttt atgttattat attatcatcc ttttgtttaa aagtcaattt tttctatttt
    16981 tgttgtttaa ttttatttat actcttttta tatttttcat tttcttttat ctttgtaagt
    17041 actccatata agttgacttt tctaaataca gtagttataa ataccaatcc taatgcaatt
    17101 gaacttgcac acgataatgt actcacccca tattctacag cttttatata attattggtc
    17161 aaaacagaat acatagtatt atatattcca taacctggca ctaatattat taaacagcaa
    17221 acacttaatg tagttgctgg agtcatataa attcttgcac ataactcaca atatatcgca
    17281 aaacaaactg tagctataaa aaaacataag ttttctgtta ctcccatatt taaaagaaat
    17341 ttatacgaaa accaagctat acttccaccg attcctgcta gtattaagtt ttttcctttc
    17401 atattaaata gcactccaaa gcttaaggca gtaaaaaaag ctgctattac ttctataaac
    17461 aaatgcactt atatcccccc taacttgaag tacaatccaa gtaccaagaa cattcctact
    17521 gctatagata cacctacaaa aaatacttct gctaattttg caagaccaga aatcaactgt
    17581 ccatctaaca aatctcttaa tgcattagtc aaagcaagcc cgggaactaa gagcattatt
    17641 gctcctgcta ttaaatggtc taacgtcttt atgaatccga gctttaaaat taaaattgaa
    17701 caagaagcta taataactgc tccaattgca tttacaaaaa agtcattaag ggaagattta
    17761 cttaaataca taataacaaa cttagtcaga atcccaacag taaatgcagc tataaattct
    17821 tccacatttc ctccaaataa tatcgaaaaa gttgctgctg caatacctgc aaaaaatatt
    17881 gttattaaat ccgagtaata gttatcttca cttatttcat ctaaaatttt ctcacataat
    17941 tcaatatcta aattatttat acttattgct cttgataatg agttaacttc atgaaccaag
    18001 tttaggtcta ctcttcttga gcttattctt ttaattgctg aatgtaattt cccatctaca
    18061 aatacagatg ccattacagc tgttggagtt gcaaatacat ctacgtggtc tattccaaaa
    18121 gattgacata ttctagctat cgtttcttca actctatagg tttctcctcc actttgcaac
    18181 atagctttac ctgcatttga ggaaaagttt aaaattctat ctatatccac aaaacatatc
    18241 tcccattctt ttatagtatt attcatattt taatacaaga tattatttat ttaaactatt
    18301 ttaaataatt catatccaac atttacattt tatgaaaaaa cttgtttttt atgcttgtca
    18361 aaatcaaatt ttaagcttgt ttacaccata aaatggctga attaatattt gacaaacact
    18421 ttcaaccatt tatataacct tctaaattgt ataacttttt actacaaata aaattatatc
    18481 tcttttaatt ctttctcttg agtatttagc ttatagtaac ttcatattta tatatgtaca
    18541 atataaaaca ttatttttat gatacaaaat ata
//
LOCUS       DAMKMI010000077        18469 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMKMI010000077 DAMKMI010000000
VERSION     DAMKMI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.078"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            169..1329
                     /locus_tag="PC429_002339"
     CDS             169..1329
                     /locus_tag="PC429_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF3847840.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1672..1833
                     /locus_tag="PC429_002340"
     CDS             1672..1833
                     /locus_tag="PC429_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847841.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2026..2655
                     /locus_tag="PC429_002341"
     CDS             2026..2655
                     /locus_tag="PC429_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF3847842.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMGGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2895..4154
                     /locus_tag="PC429_002342"
     CDS             2895..4154
                     /locus_tag="PC429_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF3847843.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4413..5408
                     /locus_tag="PC429_002343"
     CDS             4413..5408
                     /locus_tag="PC429_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF3847844.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5654..6721
                     /locus_tag="PC429_002344"
     CDS             5654..6721
                     /locus_tag="PC429_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF3847845.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7255..9165
                     /locus_tag="PC429_002345"
     CDS             7255..9165
                     /locus_tag="PC429_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF3847846.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9228..9851)
                     /gene="ytaF"
                     /locus_tag="PC429_002346"
     CDS             complement(9228..9851)
                     /gene="ytaF"
                     /locus_tag="PC429_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF3847847.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10039..10683
                     /locus_tag="PC429_002347"
     CDS             10039..10683
                     /locus_tag="PC429_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3847848.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10897..11955
                     /locus_tag="PC429_002348"
     CDS             10897..11955
                     /locus_tag="PC429_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF3847849.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12059..13243
                     /locus_tag="PC429_002349"
     CDS             12059..13243
                     /locus_tag="PC429_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF3847850.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13450..14163
                     /gene="rpsB"
                     /locus_tag="PC429_002350"
     CDS             13450..14163
                     /gene="rpsB"
                     /locus_tag="PC429_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF3847851.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14242..15153
                     /locus_tag="PC429_002351"
     CDS             14242..15153
                     /locus_tag="PC429_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF3847852.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15238..15942
                     /locus_tag="PC429_002352"
     CDS             15238..15942
                     /locus_tag="PC429_002352"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF3847853.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15968..16525
                     /gene="frr"
                     /locus_tag="PC429_002353"
     CDS             15968..16525
                     /gene="frr"
                     /locus_tag="PC429_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF3847854.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16528..16731
                     /locus_tag="PC429_002354"
     CDS             16528..16731
                     /locus_tag="PC429_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847855.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16760..17494
                     /locus_tag="PC429_002355"
     CDS             16760..17494
                     /locus_tag="PC429_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF3847856.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17507..18286
                     /locus_tag="PC429_002356"
     CDS             17507..18286
                     /locus_tag="PC429_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF3847857.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7591 a         1850 c         3219 g         5809 t
ORIGIN      
        1 aattaatgtg caaatcttta ttaatatatg tcaatagtta ggtatatatg tatttaatta
       61 gcatgatata gataaaagta tttattttaa ttgtctatta tctgaaaaag tctttactta
      121 gaaatcataa aaatcaaatt aattaggaaa agaaagggtg tgtataatgt gattgagact
      181 ggatttgtat attgtagttt cttggttgta ttcgtaggtg taattttttt tatggttgat
      241 aaattgaaat ggaaggtatt tgatgttatt aatccaatgc ttttaatata cttaggttca
      301 gctatacttg gatcatttgg tttatttgca cagaatgaag atgttggaac gtatcaaaac
      361 ataataacag tgaacttcct tccattaatg ttagtgtttt tgttaattca atgtgattta
      421 agaaaaatat taaagatggg gcctaaaatg ttattatcat ttttctgtgc tacatttaca
      481 tttatgcttg gatttgttat agcattcata atttttaaaa attcaatggg tgcagattca
      541 tggaaaattc ttggggcagc atctggttca tggataggtg gctcatcaaa tatgattgca
      601 gcagctaatg ctctaggagt tgcagaagaa ggacttagtt atgcaatact tatgggttct
      661 ataggatata cagtatggat gtctgtttgt ctaatgtctg tgaaatttgg acctaaattt
      721 aacaaatgga caaagtgcga tacaagtttt atagatgagg ttacatcaaa acttgaaaaa
      781 gaaaataaag ataatacagc acctacgttt gtagaactta tgattttact ttctgttggg
      841 tttggagttg cgtcttttgc acaaattgta tccaagatac ttccaacttc aggtatactt
      901 aatgaaacta tgtgggtagt acttgttgca tctacagtat caatattatt gggtatgaca
      961 aaaatatcaa agcttaaagg gagtacactg gtaggaaatg tgtttatgta tttactcctt
     1021 gcagctacag gtagtaaagc aaatttcttt ggattgtcag aagcacctgt ttacataatg
     1081 tttggattgg tagtagtcct tgtacatgct atttcattta tagggcttgc taaactattt
     1141 aaacttgatt tatttacttg tgaagtagga agtatagcta atataggagg agtaactaca
     1201 gctccaatga ttgctggggt tcacaatcct gtattaatac cagtaggtgt acttatgggg
     1261 cttctaggaa atattatagg gacttattgt gctcttatgg taactcaaat attacatatg
     1321 ttagcttaat gaaaatatgg tataaaatta ataatttaaa taagaaaagt catttcaatt
     1381 taagcagaag cttttactga aatggctttt tttgtttgac aagataatct atttcaagaa
     1441 gatgttttga catatttagt gtataatatt gtaaaattta ctaaaaacga gatatattta
     1501 atttttatat tgaatcgaac gtctgtatga tgtataataa atatacagaa cgaatgtttg
     1561 cggaacgggt gttttttgaa tttattttaa taatttatga atatatatca gaatgcataa
     1621 atatatttaa atataaagat taaaaagaat ttaaaggagt gttgataact tatgataatt
     1681 ccatcaagaa gaatacaaga tataacatta acaaatttaa aaaatggtga agtaacttta
     1741 attgaattgg atgaaatata caagaagatg ggatttttat ttgttgttag tgaaggcaaa
     1801 ttgaaaaaga taaaaaagga gaatcatcat tagaaatgaa tgaaataata aatattataa
     1861 tagtttataa aacctaactt atgtagaata taaatactta agttaggttt ttttgtgata
     1921 aagtttataa gtactcaatt ttataagata aataatggtt ttaagtgaaa agtaaataag
     1981 tttaaatttt agtaagtatt atattgcata agaggtgata ttattatgaa aatagtaacc
     2041 tacaatattc ataaaggtat ggattcaaat aatagattaa ctctaactaa aatggggtta
     2101 tatttaaaac aattggattg tgatgtaatt tgtttgcaag aagtattata tcctcaattt
     2161 ttagctttaa aagctgtttt aaatatgggt ggtgtctttg ctacaaatgt aaaaaaagta
     2221 aatatgatat atggtatttg tacatttaca acatctaaaa tgttaaacag taatcatttt
     2281 ttcttaacta gcaaaaaaga acaacgggga gctctttgta taactataga tgcatatgga
     2341 agaattataa atgtaataaa tactcacttg ggacttgata ggcaggagag agcaaaacaa
     2401 ttagacgaaa taatagacta taggaacaga ttagtgggga tagttatttt atgtggtgat
     2461 tttaatgaga aaaatgtctt tttaagtatg tttaatgata tggctatttc actaaacaag
     2521 tcttacttac caacatttga aaaatcaaac tctagaatag attacatttt tgttaataaa
     2581 aatactgaac taaaaggata cacagtagaa aaaatttacc tgtcagacca ttatcctgta
     2641 ataggttaca tttaaatttc aatataaatg aaattagttg ttataaaata aacaattttt
     2701 ttgttaagaa atagtaaaag aaatataata aaaaatatca aaaataaaac taattataag
     2761 acataaattt acaataaagt tacatttgat taaattcctt gtttaacaaa agctattcaa
     2821 atataatcta aggtagtttt gaaatgggaa ctctattatg caaatttaag ctagtgttaa
     2881 aatgaggtga agttatgggc agaagaacaa caagagtttc aagattaggt aaagaaaaaa
     2941 aacgaagtgt tttaattgta ttggcagctt tagttctaat aatgggtgga tttacttatt
     3001 tttttaacag taaatttctg tacaatggga aaatcgctaa aaatgtatat atagaaggag
     3061 taaatgtatc tgatatgact aaagcagagg cattaaaagc tataacagat aagtatactc
     3121 cagaagactt aaatcttgct tatgatggga aaaaatatac aatatcacca aaagatattg
     3181 atttaaaata tgatacagaa gatgttgtaa aagatgctta tgaaagtaca aaaaaagggt
     3241 cctattttca aaacttaaaa aaatatattg atataagagt gaataaggca aatatgaaga
     3301 taaaagctga atatgatgag gcaaaactta gttccaaagt gtcttctata gcagatagta
     3361 taaatgttaa gatgaaaaat gcttctatta gtgttggaag tggtggacta agttatacag
     3421 actctgttgt aggtagagaa tttgatttgg cagcaaataa agagtctata tataatatga
     3481 taaaaaataa agagcataaa actttagaac ttaaagtaaa tcttcaaaaa ccagatataa
     3541 caactgaaca agtaaaaact gtaaattctg ttataggtca atattcaact acttattcac
     3601 aagctgtaga gggtaggtca tataatgtag gattatcagc aagaaaaact agtgatgtat
     3661 tattaatgcc aggtgaagaa ttttcataca ataaacttac aggacctagt aataaagcaa
     3721 atggttacaa agatgcacct gttatagttt atggtaagct agaacaatca gcaggaggag
     3781 gtgtttgtca aacatcttca acagtttata atgctgctct tctttcgggt atggagataa
     3841 ctcaagttac aaatcattca tctgcatcta cttatgtgcc taaagggaga gatgctactg
     3901 tgagcgatgg aggattaaac ttaaagttta aaaatccata taagcatcct gtatatatta
     3961 aaaattatgc aggtggagga agtgtatcat cagtaatcta tggtaattct ggtgacaaac
     4021 caaatatatc tatagaggta aaacaaactg gtcagaataa atattctaca tatcgaatat
     4081 ttaaagattc aagtggaaag gtcataaaaa aagaacatat atcaaatagt tcttataaag
     4141 aattaaaaaa atagataatt ttttagagaa atatttgtaa tataaaaggg agtgtctcaa
     4201 aatgaacttt ttagtttatg aggcactctt tttctgtgaa atcaaatata ttttcatcat
     4261 ttcaacaata caaaagaagc gtatctctat tttgagacag ccttttttgt aaaaattaac
     4321 actattagtt cttgttttaa tattacaatc attaatatat agaaaatatt cctatatatt
     4381 ggtataattg taaatagatt tagttatata aaatgtatga ggaggtgaca tggttgaatt
     4441 ttataggcaa tagatatgag gtagtaaatt ctgatgacgt tttagaaatt aacaaaatct
     4501 ataaagctag agatgttttt tacaggaaaa atgtgttaat aaaagttata aagcacaata
     4561 attatatctg tgaagatttt gtttcaaatt taattgatga aagtactgct ttagatgaga
     4621 taaactctcc ttatatacta aaaataatag atgttgggat tcactgtaca gaagaaacaa
     4681 cattatatta tgttgtaagt gaagacttta atggtatcgg actagatgaa ttaattctag
     4741 gtaattatct tcatttagaa gcaattgtaa acataatgat acaagtttta aaagctttag
     4801 aaatgataca taggaatcat tcatatcatg gtagtttaaa atcgagtagt ataatagtag
     4861 atacagaata caatataaaa atttgtgatt ttggaattac aaaagcaaat aatggggtta
     4921 atacaagaag ctatggaaac aggagatatt tatgcccaca tcaactatgt ataaattata
     4981 ctgataaaga aagtgatttt tttgcaacag gtcttattct atttgaatct atatttaaaa
     5041 aattgccttt tggagaatca aattcagaag aaaaaatgct acaatctatt gataagggat
     5101 tagattggaa tagtataaaa gcaattaatg gaaatactga acttataaat gttataaaaa
     5161 ggcttcttgg aagaaataat aagtatacta gagccaatga tattattatt gatttaagta
     5221 aagtaatgta tgaaaaagca tatatagaag ttgaagatga gaaagaagaa gagcagaaac
     5281 aagacaaagt tatacaagtt aaaaaagaat ataaaagtaa aatgagaaaa aaattacata
     5341 aaaaacttgt tgttgccggt ctagctatag tcatgatttc aatgattatt attagttcca
     5401 taatataaaa attgtatatt aagtatttta aaatcaatgt ctataaagga tatacataga
     5461 aattttagtg tagtcttaaa ttatgaaagg aagtggttat tatgttaaca aaaaataatg
     5521 aggtattaaa cgtctaggag tattttaaca aatagccacc tgcttttaca gtttagaata
     5581 aaaaagctgt ggctaaaggt cacagctttt tgctactttt taaaggcagg agaatattaa
     5641 aaaggagcaa attatgagca atataaaaat aatagcaagt aataaatgtt ggattgaaga
     5701 tatagcaagg aaacaacttg aagatatttc aaagttaaat ggaattttaa gagtagtagg
     5761 attacctgac ttacatgcag gtaaaatacc tgtaggttta gcagttgaga ctaaaaatat
     5821 tatttatcca catataattg gaaatgatat aggatgcgga atgactttat ttaaaactgg
     5881 ggttctaaaa aagaaattta aaaaagacaa atggattaag tcacttagta agattaaaga
     5941 tttgtccgat atagaaataa aaaacacata taaagaagaa tgtccaattt taaatttagg
     6001 aactataggg agtggaaatc attttgtaga aattcaatgt ataagtgaaa tttataataa
     6061 agaacaattt gaacaattaa aattttcatc tgatgatatt atgatgttag ttcactgtgg
     6121 gtcaagaaat tatggagaag atattttaaa aaaattttat gataaagatg gacttgaagt
     6181 tgaaagtgag aaagctatag attatattaa aaaacatgat aatgctttaa tgtgggcaga
     6241 gcgtaatcgt gaagctatca gtaaaaaaat tatgcagtca ataggtacat ctggagatgt
     6301 tgaaacaata ttctctataa atcataattt tatagagaaa agggaagata aatttataca
     6361 tagaaaaggt gctgtttcaa gtgaaagagg agctgtgatt ataccaggtt caagaggcag
     6421 tctatcatat atagttatgc ctactgaaaa tacagagata tctttatatt ctctatcaca
     6481 tggagcaggt agaaaatggt ctcgttcagt ttgtaaatct cgccttaaaa gtaagtataa
     6541 taaagatact ataaaacaaa ctaaatttaa aagtcagatt atatgtaatg atgtaaattt
     6601 attatttcaa gaagctccag aggcatataa gaatatagag cagattattg aaagtttgat
     6661 agaatatgaa cttattcaag tagtggcaac tatgaagcca ttgattactt ataaaggata
     6721 atttttaaag ttaaaaaaga ttaaaattat attataaata gctaatttta tggaacattt
     6781 aaaagtgttt tattattagc tattttttta ttttgtagag acgataattt atagagctaa
     6841 attctttgat atataatgat tacaaagtta gactgtattg gaaaatgcac atacatgaat
     6901 tgatgacaat ataagtgatt cccaaatata aatgaaagta taagcttatt ttcactataa
     6961 aaattttcac tgtacaaaaa cattaatgac attattttta ttgacataaa tatataattt
     7021 atggaataat attagaagca ctgatataag tgtttaaata agaatagaaa aggagaagtg
     7081 ttttatgaaa aaaaatacta acattaatga gtccggagtt aaacttctta ataaataagg
     7141 agtttgatat ataataactc cttatttatt aagttgttcg caaaataact attatcaaag
     7201 taaatgatag ggtgatttaa aagacgataa cttataaata aaggagaaaa aataatgaat
     7261 agtaatacat ttgaaagatt acaactaaat gaagttaaag aattaataaa aatacattgt
     7321 gtaagttcac taggaaaaaa tttaatagat aaattaactc ctagtgggaa tattggagta
     7381 gtaagaagga aattagcaga aaataaagaa gctagaaaga taattgaaaa tagtaatcac
     7441 ataccacttg aaggattgtt taatgcaggt tctacaatag ataaaattga aaaaggaatg
     7501 ataatagaac ctgttgaact tgtaaatatt gaagattttt taagaggttg taggaagatg
     7561 aaagctttta tgttagaaaa agaattttat tcacctacat taagttctta tgccttaaat
     7621 ataacagaat gtaaaagtat agaagatgag ataaattact gtataaaatc taataaagtt
     7681 gattctaatg ctagtaaaga attaaaaaag ataagacgaa atatagaaat tacagaagga
     7741 aagataaaag atagattaaa taaatttata acatcaactg taaataaaaa gtatatacaa
     7801 gagtttataa taagtaaaag aaatgataga tatgtaatac caattaaatc ttcttataaa
     7861 aatgaagtta atggtactat attagataca tcatcaaaag gaaatacggt atttattgaa
     7921 cctattagtg tttcaaattt aagtacagag ttaactatgt taaaagctga tgaaactatt
     7981 gaagaataca aaatattatc ttatttaaca gaacttatat ttgagaagat aagtcagata
     8041 aaattaaata tagagatact ttcagaatac gatatggtat ttgcaaaagc taagtatagt
     8101 caaaaaataa aaggaattac tccaaaaata aataataatg gctatataaa aataattaaa
     8161 ggtaaacatc cacttctaac aggagatgca gtacctttag attttgaaat aggcaaaaat
     8221 tatagaagtc ttataataac aggtcctaat gcaggaggaa aaactgtaac ccttaagact
     8281 gttggtttac taactttaat ggttcaatgt ggatttgata tttctgcaaa agaaggtagt
     8341 gaatttagtg tatttgaaaa agtttttgta gatatagggg ataatcaaag tattgaaaat
     8401 gccttaagta ctttttcatc acatataaaa aatattgcag agattatgag tttatcaaat
     8461 aattctactc ttgttctgtt tgatgaaata ggaagtggaa ctgaacctaa tgaaggagct
     8521 ggtttagcaa tatcattatt agaagagttt tacaaaatgg gatgtataac tatagcatca
     8581 actcattatg gagagataaa aaagtttgct acattgcatc cagaatttga aaatgctggt
     8641 atgatgtttg ataaagaaac gttagaacca ttgtataagt taactattgg aaaatctgaa
     8701 gatagtaatg ctctttttat ttctaaaaaa atgggaataa aaaatagagt tttagataga
     8761 gctaaagaat atgtaattga tagaaactac aatttagatt tagttaaaag aagtaagcta
     8821 caatctaata ttgaagataa aaatagttct gaagtcattt taaactatac agattataat
     8881 gtaggggata aggtaaaatt actagatgaa gatgattttg gaattgtata taagcctatg
     8941 gacaagttta ataatgtaga agtattgttt aatgataatt ttatttcagt aaatataaaa
     9001 agattacaac ttagcataaa agcatcagaa ttgtatccag aagggtatga tttagatgta
     9061 ttatttacta gttttaaaga aagaaaatta gaaaaagata ttcaaagagg ttcaaaaaaa
     9121 gctcttaaaa agattcagaa agaaattaga gagaatagaa aataaatata aaaagcacaa
     9181 aagaaagcaa tctaaagaga aaagtttatc tttagattgc tttcttatta acatatcttc
     9241 ataaatccaa tgactaacaa gattaaacca cttaaccaag ataagtttaa atttacgttt
     9301 ttaacaaaca tatttcctag taaacaacca agtaatactg caattatatt tgatattaaa
     9361 gaaaatagaa taacttgtat ataattcaca cctacaaggc cactaccaaa accgatagct
     9421 aatccatcta aacttaaagc aagtgcaagt gtaaatgctt cagcagattt aagcacttta
     9481 gatttacttg tatctaagac atcaggttca atacttacat taaaatttaa tttaaggtct
     9541 aacatattaa aattataatt ctttgagtgt atagattttt tcttaaaata agatttaaaa
     9601 aaactttcaa ttagtctagc acttcctaat aaaaataaaa taaagaaaca tattaacatt
     9661 gttatatttt gaggtaaaaa gtctctaact attgtacctg caaaaagcga tattgctaaa
     9721 atagctgaac aaactacgct gataattata tttgaatata agggaacttt aactttattt
     9781 acgccatatc caaagcttgt cacaaaggca tctatacaaa gagaagtaac taataaaatt
     9841 gattcaatca cagtagatta acatcctttc tcttaaattt tgtacttata ttagttatag
     9901 tattgtaaaa atggataaat tgttacagat aatccaataa attgttgata aaattccaac
     9961 atcatacaac acctaaataa caaaagaatc atatcatata ataatataaa accaatcata
    10021 ataaagcagg tgaaaataat ggaacaacat agtaaggttg ggaatttcgg tgatttgttg
    10081 aaagttttat tagatgaaaa agaattatca atgggagaac taagtaaaaa aagtggaatt
    10141 gataaatcta ctatatctcg tatagctaat aataaacaaa agccaaatat aaatcattta
    10201 gaaaagatgg caatacattt aaatattaac ctagaagaac tgttgaaagc ttcaggatat
    10261 gaatttagaa acagcaataa tcaaatattt aatgcagata gtgattttag taactttgat
    10321 gatattcttg gatttgcaaa tttaatcaat gataagaact ttaatggaaa tattgaaaaa
    10381 gagttaagta agtgtaagtt atatgtccaa acagatgaag gtaaaaaatt actttttgat
    10441 aattttaata aaaaaataga ttctattgaa aaacaaggtc aatttacaga tagattaaga
    10501 caaatgtatg ctgatttttg tacagatgga cttacaataa agaaatattt attgattgga
    10561 agtatgctac tttactttgt tatatcaaca gatgtgattc cagacttcgt gttcccaata
    10621 ggatttatgg atgatttagt agcattaaat atagttacaa aattacttaa aaatgataag
    10681 taagttttag aatcatattt aatatataaa taaatcaaag tatataaaat ttattatttt
    10741 ttatatgaaa caaattaaaa gcacaatgaa gtagattaaa aatttaaaac taagaatcta
    10801 atatatattt tgtaactatt taagtatata aaataaaata atatccaaac taatacaaaa
    10861 tagtaataat tgttttgtac tggaggatat atatttatga attgtaaatg gatatctaaa
    10921 atagatgaga gaaaaaaatg tcatagagaa gcagattctt ctggatattg tatctttcat
    10981 aaagaaaata agtctgatga agagatacaa ttgatgatgg atactcttca taaagaagag
    11041 ataagtgaat ttaatggatt tgtttttgaa aatgaattta atgcggaaga aatattaact
    11101 tacaattata aaatactgga tttttctgaa tccatattta aacaaaaagc aaattttaaa
    11161 aaatatattt ttaaaaagaa tattattttt aactatacag aatttagaga taaagtttta
    11221 tttaatggtt gtgtattctt agaaaactgt gattttaata gaaccatatt tagtaaacac
    11281 tatataaatg accgtatatt tgaaaaagtg aaatttaaag gacctgattt ggtagttaat
    11341 aaagtagaaa attttccacg aatggatgga ataattttta gcatgtgtac aaaatttgtg
    11401 ttaaagaatg ttgaatatgg aaaaagtgag tatgagcatg gcaagataaa ttatagaata
    11461 gctagaaatc aagcaacaaa aattggagaa tatgagatga taggatttta ctattataag
    11521 gaaagaattt atagtagtaa aataatgaaa cgttctaatt atcctacctt cagtgattat
    11581 ttagttgaaa aattttttga ccaaatagca cgttacacaa caggttatgg agaaaagcca
    11641 tggaatatat tattagttat tatagcaata ataagtgttt ttgctttatt atacttattt
    11701 gtaggcatag aaagttcaaa tagcacactt gtagctttag atataaataa tataggtgat
    11761 tactcactaa gtgagatttt caaaatgtat atggatttat ggtattttag catggctaca
    11821 tttagtacag taggatatgg agatatggtt gctacaagtt tgattgggaa agcattggca
    11881 ggtatagaag tattttttgg agttacaata ggagcaattt gggcatctgt gattattaaa
    11941 agaatgataa ggtaatattt gtgtaaatat ggataggtat taattactag atataaattg
    12001 gactaaatag ggaaaattta aatataaata tacttaataa tccaagaagg tgaaaaattt
    12061 gaaaaatgca ctaaaaaaaa ttatatcatt agccatgatt ctagttttta taataccaaa
    12121 ttattcatat gcagatgata aatctgtaga caagtattca aaaacatcta tacttataga
    12181 ccaagaaaca ggtagagttt tatatagtaa agaacctgat atgaaggtac cattagctag
    12241 tttaagtaag atgatgactt ttttacttgc aatagaagct attgaaaata aagaagtaaa
    12301 agaaactgat aaggtaaaaa tagacaaatc tattgcatca gtaaaagggt caagttataa
    12361 acttaaagat ggtgaagaag tgccacttat agagcttatg agaggtctta tgattgtatc
    12421 aggaaatgat gcggcaattg ctatagcaaa acatgtttgt aaaacgaaag aagcatttgt
    12481 agatagaatg aataaaaaag caaaagaaat aggaatgagt aatacacatt ttctaaatcc
    12541 aaatggtctt ccaatatacg atttaagcaa ccccaaaaaa ccagcaaaag aaaatatctc
    12601 tactgcaaaa gatatagcta ttcttggaaa atacatgttt gaccattatg aaaaacaagt
    12661 aacagctata actgatatgg agacatatac aaattcagat agaagttttg aaaaaagcaa
    12721 tacaaatgca ttattaagaa ttataccaga agtagatggt ataaaaactg gatatactgg
    12781 caatgctgga tattgcttat cattttctat ggttgtaaat aaaaatgata aaaatgagaa
    12841 aaaacgtaga gtaatagggg ttgtattagg tactaaccat aaaaataaaa gaacctctgc
    12901 ttcattagct ctactgaaat atggaaaaga aaatttcaat atgaaaaaag taattgataa
    12961 agatagcttt attggtaaga aatatataaa aggaacgaaa gagttagagg ttacattaaa
    13021 agcaaaagat gaactttata caatattaaa agatgatgaa agtataaagt cagaagttaa
    13081 gattaaaaat atagagtacc ctgttaaaaa aggtgatact atgggtatta taaaatatta
    13141 tactaattca ggtgacttac ttggaagtgt agatatagta agtaataata gtatagataa
    13201 cgtatcatta aaaacaaaat taaaaatgaa atttgcaaat taaattcaaa aaatggtata
    13261 aaatacacaa aaaaatataa aaatagaaaa tatggaaaaa atattgttat ttcttcacct
    13321 ctgtgatata ctactagagg tgaaaaaata aaataaatca cacacagcat tgggatcctt
    13381 aaaaggtgcc tattacatgg ttttttaggg agaagatcaa gctggaggta taaaaccagg
    13441 aggtaagata tgtcagtaat atcaatgaaa caattattag aagcaggtgt tcactttggt
    13501 caccaaacaa gaagatggaa ccctaaaatg gctaagtata tattcacaga gagaaatgga
    13561 atatatataa tcgacttaca aaaaacagtt aaaaaagttg aagaagctta caagttcact
    13621 aaagaagtag ctgaaacagg aaagccaatc ttattcgtag gaactaaaaa acaagctcaa
    13681 gatgcgataa aagatgaagc tgaaagatgt ggaatgtact ttgttaatga aagatggtta
    13741 ggtggaatgt taacaaacca caaaactata aaaacaagaa taaataaatt aagagaatta
    13801 gaaaaaatgg aagaagaagg cgtatttaat gttcttccta aaaaagaagt tataaaatta
    13861 agagctgaaa aagaaaaact agaaaaatac ttaggtggaa taaaagacat gcctgaatta
    13921 ccaggtgcaa tgttcgtagt tgacccaaga aaagaaaaca tagctattca agaagctcat
    13981 agattaggta taccagtagt tggtatagtt gatactaact gtgacccaga acaattagac
    14041 tttgctatac caggaaatga tgacgctata agagccgtaa aattaataac tggtgctatg
    14101 gcaacagctg taattgaagg tagacaaggt gctgaggaag aagtagctga agaccaagaa
    14161 taataatttg acaattaaat ggtaagggtc gcccttacca tttataatat ggaatagaat
    14221 aagtaggagg aaacttaaaa aatggctaat ataactgctc aaatggtaaa agagttaaga
    14281 gaaagtacag gcgcaggaat gatggactgc aagaaagctt tacaagaagc tgaaggaaac
    14341 atggaaaaag cagtagattt attaagagaa aaaggattat caaaagctgc taaaaaagct
    14401 ggtagagttg ctgctgaagg tctagttgca atagaaatga atgatgataa tacagttgct
    14461 tctatggtag aagttaactc agagacagat ttcgttgcta aaaacgaaga cttcaaagtg
    14521 ttcgttaaag atgctgcttg tatggcatta gctactgata aagaagatat agcttcttta
    14581 ttaggtgaaa ctcataaaga aggaataact ttacaagaag ttttaaataa tagagttgca
    14641 aaaataggtg agaaattaga ctttagaaga tttgctaaag ttgtaactaa tggacaagtt
    14701 gctggataca ttcacggtgg tggaaaaata ggtgttcttg ttgaaatgga aacagaagct
    14761 agagatgcta aagttttaga attaggtaaa gatgtagcta tgcaagttgc tgctatgaat
    14821 cctaagtatg tttcaagaga tgaagtagat gctgaataca tagcacatga aacagaagta
    14881 ttaactcaac aagctttaaa tgaaggaaaa ccagctaata tagttgaaaa aatggttaaa
    14941 ggaagattag aaaaagaatt aaaagaggtt tgtttattag agcaaacttt cgttaaaaat
    15001 ccagatataa ctgttaaaca attagttgct gatgttgcta aggcagtagg ttctgatata
    15061 aaagttgtta aagttgtgag attcgaagtt ggtgaaggta tacaaaagag agaagaaaac
    15121 tttgcagaag aagttgctaa gcaacttaag taataaccaa agagagaaca cgcaggtgtt
    15181 ctctttttta aaaaatagaa aaaattggat atgaaggatt gaaataggag gtttctgatg
    15241 gataaaccaa tgtacaaaag ggtattgtta aaattaagtg gagaagcatt agcaggagaa
    15301 agaggttttg gtataaataa tgatgtagtt aatgatattg ctattgctat aaaaaaaata
    15361 caagaaatag gtgttgaagt agctgtagta gtaggtggag gaaacttctg gagaggcaga
    15421 accagtgaag gaatggacag aactactgct gactatatag gaatgttagc tacagttatg
    15481 aatgcaatgg cgctacagga tgcactagaa aatatagatg ttgctacaag agttcaaact
    15541 gctatagaca tgagacaaat tgcagaacca tatataagaa gaagagcagt tagacactta
    15601 gaaaaggaaa gagttgttat atttggtgca ggaacaggaa acccttactt tacaacagat
    15661 acaactgctg ctttacgtgc tgctgagatg gaagcagaag ttatattgtt agctaaaaat
    15721 gttgatgcag tatatgacaa ggatccaaaa gtacatgcag atgctaagaa atttacagaa
    15781 ttaagttaca tggaagttat acagaaagaa ttaaaagtaa tggattctac agctacatct
    15841 ttatgtatgg ataataaaat tccaataaag gtatttgagc ttacaacaga gaatataata
    15901 agagcagtaa aaggcgaaaa tataggaact actgtaaaat aatgaagaat gaaggaggag
    15961 taaaattatg aaactggaaa tacataagca attagaagag aaaatgaatg ggacaattga
    16021 tgctttaaag tttgaatttg gaactataag agcaggaaga gctaatgcac aaatgctaga
    16081 caaaataaga gttgattatt atggaactcc aactccaata aatcaaatag gtgctatatc
    16141 tgtaccagaa ccaagaatat taatgatatc tccttgggat aaatctgcta tgcatgaaat
    16201 agaaaaagcg atagctaact cagacttagg attaaatcca tcaaatgatg gtgaggttat
    16261 aagactttct gtacctgctt taacagaaga aagaagaaaa gaattagcta aaaaagctag
    16321 taaagctgca gaagaattta aagttagaat aagaaatgaa agaagagacg ccaatgagaa
    16381 aattaaaaag atggaaaaag gcggagaatt aactgaagat gaattaaaaa aagcacaaga
    16441 tgaagttcaa aaaatgactg ataaatttat aaaagaaata gatacattat tatctaaaaa
    16501 agaaaaggac ataatggagg tataatattg aaaaaatatt ataacctttt aggtcttcat
    16561 gtagatgagg taaaacaata ttttgatgag caaaatataa attataccgt aaaatctatt
    16621 gaaggtaaaa aggataaaga aaaacttgta gttcctagag ttataaaaat atctgagata
    16681 ggtgatagtg tagaactgat aatcacatac ttttctgact ccttagttta aggagtcaga
    16741 acaatattgg aggcaaaata tgaataacaa cattatatat gatatagact taaataacat
    16801 acctactcat attgctatta taatggatgg aaatggaaga tgggcaaaag ctagatttct
    16861 tccaagaact gcaggacata aggctggagt agaaactata agagatatag taaaagaatg
    16921 ctcaaaatta ggtgttaagc acttaacatt atatgcattt tcaacagaaa actggaaaag
    16981 accgaaactt gaagttgaca cacttatgaa cttattatct acatacttaa gaaatgaaat
    17041 agctgaactt caccaaaaca atgtaaaagt aactgctata ggagatataa gtgctttacc
    17101 aaaaacttgt ataagagaat taaactcagc taaggaactg acaaaaaaca atacaggagt
    17161 taacttaaat ttagcattaa actacggaag tagagcagat ataaaaaatg ctcttataga
    17221 tatagtaaaa aattgtgaaa gtggtaaaat agatataaac aatattgatg aagatattat
    17281 aaaaaactat ttaagcacaa agtctatacc agatccagac ttggtaataa gaacaagtgg
    17341 tgaacaaaga ttgagtaact ttttactttg ggaagttgct tattctgagt tttactttac
    17401 tgatatacat tggcctgatt ttaaaaaaga agaactacag aaggctatat acgtatatca
    17461 aagaagggat agaagatttg gaggacttaa gtaaggagat tattttatgc ttacaagaat
    17521 tattgcttca ctagctctgg ttccattatt tctatttgtt gtatatggag gtattcctct
    17581 atatatagct gagacagcaa tagtttatat tgcattgcat gagttttata aggcatttaa
    17641 aataaaagat gtacatccaa tattcattat aggatattta ttttctattt atttagctgt
    17701 aaaaaatatt tttaatttac cattagaata tacttatgca gtaattttta ttttattttt
    17761 agcaagtata atttatatgt tgatgggcaa aaacaatgtt atagatgttt cgattacgtt
    17821 tttaggagtt ttttatatag gagtattttt agattttata attataacta taaatggatt
    17881 tgaaaaaggt agtatatatg tatggcttat atttgtgata tcttttatga cagatatatt
    17941 tgcatatttt agtggttatt tacttggaaa acataagcta attcctaaag ttagtcctaa
    18001 aaagactata gaaggagcta ttggaggaat aataggaagt acactttgtt gtattttatt
    18061 tggatattta tttggcattg atttgcttca attagcgata ataggaagta taggaagtgt
    18121 aatagctcaa ttaggagatt tatttgcatc ttctataaag agatatgtag ggataaagga
    18181 ttatggaaag ataatacctg gtcatggggg tatattagat agatttgata gtgttatatt
    18241 agttgcacct tttgtatata gtgcgattaa attttttatt agataaagat tggttggatt
    18301 taaaagagca atctataatt gctagtaatg aaaatagcta tgtaactttt aaatgcagag
    18361 atattctgca gttattagtt acatggctat tttcattgtt caaaaattta gaagaccttg
    18421 gggaattgta taagaatagg tattacttta taatagtata ctatatttg
//
LOCUS       DAMKMI010000078        18397 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMKMI010000078 DAMKMI010000000
VERSION     DAMKMI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18397
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.079"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(202..882)
                     /locus_tag="PC429_002357"
     CDS             complement(202..882)
                     /locus_tag="PC429_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847858.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(932..2947)
                     /locus_tag="PC429_002358"
     CDS             complement(932..2947)
                     /locus_tag="PC429_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3847859.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(3019..3696)
                     /locus_tag="PC429_002359"
     CDS             complement(3019..3696)
                     /locus_tag="PC429_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3847860.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(4276..4359)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4592..5434)
                     /locus_tag="PC429_002360"
     CDS             complement(4592..5434)
                     /locus_tag="PC429_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3847861.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(5686..7470)
                     /gene="pepF"
                     /locus_tag="PC429_002361"
     CDS             complement(5686..7470)
                     /gene="pepF"
                     /locus_tag="PC429_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF3847862.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLVHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(7536..8201)
                     /locus_tag="PC429_002362"
     CDS             complement(7536..8201)
                     /locus_tag="PC429_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF3847863.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(8352..8513)
                     /locus_tag="PC429_002363"
     CDS             complement(8352..8513)
                     /locus_tag="PC429_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF3847864.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(8591..9100)
                     /locus_tag="PC429_002364"
     CDS             complement(8591..9100)
                     /locus_tag="PC429_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF3847865.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9306..9524
                     /locus_tag="PC429_002365"
     CDS             9306..9524
                     /locus_tag="PC429_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3847866.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9542..11488
                     /gene="feoB"
                     /locus_tag="PC429_002366"
     CDS             9542..11488
                     /gene="feoB"
                     /locus_tag="PC429_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF3847867.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(11667..12722)
                     /locus_tag="PC429_002367"
     CDS             complement(11667..12722)
                     /locus_tag="PC429_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3847868.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(12770..13570)
                     /locus_tag="PC429_002368"
     CDS             complement(12770..13570)
                     /locus_tag="PC429_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF3847869.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFALRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(13563..14354)
                     /locus_tag="PC429_002369"
     CDS             complement(13563..14354)
                     /locus_tag="PC429_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3847870.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(14394..14816)
                     /locus_tag="PC429_002370"
     CDS             complement(14394..14816)
                     /locus_tag="PC429_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF3847871.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(14832..15311)
                     /locus_tag="PC429_002371"
     CDS             complement(14832..15311)
                     /locus_tag="PC429_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3847872.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(15443..18199)
                     /locus_tag="PC429_002372"
     CDS             complement(15443..18199)
                     /locus_tag="PC429_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3847873.1"
                     /translation="MRDEILSFVNECVLFNSFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGIAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         6018 a         3155 c         2024 g         7200 t
ORIGIN      
        1 catcttttca aatataatct ataaaataat agttccatta cgcatttttt tatataataa
       61 aactgactgt aatttaactg gcaaagtcta gaagaatagg atttatcaat taaattacag
      121 tcagctattt tataataatt tagtttagat atcgttagta tttcacattt ataacaatta
      181 ttaatgatat taatatattt attattcctt attaaaataa tatcctgctc ctcttattgt
      241 tataattttc tgaggatttt tataatcttt ctctattttc tttcttaatc cattcactgc
      301 aactaccaca ctatagttat cctctaagta ttcattcatc catacatgtt catatatctc
      361 tttagtagtc aaagtctttt caggattctc tataaaaaag tttaacaact tattttctgt
      421 tggagtcaat tttattattt tatcttctaa ctttattact ccactagttc tattatattc
      481 ctctaatttt gtattatttt cattattatt tacatatgag ttcattcttc ttaaaatagc
      541 ttttactttt gcgtataata tttctaaatt tagaggcttt attaagtaat cgtctccacc
      601 agaatttaaa gcatttaaaa tgctttggtc ttcatttata taagtcatat aaacaattgg
      661 acaagtagtt acatttctaa tttttttaca aagagtccat ccatcaccat cacttagaat
      721 tatttctaaa aatattaaat catatttatt gctaaatatt tttccaatag cttcttcaca
      781 attatatgct aaatctatac ttattccttt ttcctccaga aatcctttta gcttctggca
      841 attatccttg tctccatcta ttatcaatat cttagtttgc attataatca ctcccaaata
      901 tcattattct tttatattaa ataagctatt attataaaaa acatagactt aattttattg
      961 taaaaacagt tccttttcct agttctgaat ctacaaatat ttctccacca tgctctctta
     1021 taatggattt gacaatatat agccctaacc cttgcccttt tggctcatta ttgtctcttt
     1081 ctgtaaagaa tctgtcaaat acctttggta tattttctgg aggtatacct tttccattat
     1141 cagaaatttt aataattgca aatccatctt ctttatccat tgataaaatt atctttccat
     1201 caaatggagt aaaggtcaaa gcattataaa ttaaattctc aaatgctctt gtaagcatct
     1261 ctttatttat ctttgcatat atttttttct ccaatacata agtttcaaag tttacaccac
     1321 atgcttgtgt atctggcaaa ttattttcat atatctcttt aataaaatta ttcagacata
     1381 ttttttcact aataccaatg cttttatctt gtgatgtaaa ttcttgcaaa ctacgtactc
     1441 tattttgtac ttcatttgac ttttgattta tagcataaag ataggttctt gtctcttcat
     1501 ctacatgaat attaccaacc tttatcaaat ctataaatgc ttgtatagcc gaaacaggtg
     1561 cctttaaatc atgtgctaca tctgccaaga atcttttacg ttcatttaac aatgttgtaa
     1621 gttcattagt tctcacttca acttcatgtt caagattcct tgtaagtttt tcattctctt
     1681 ttattattat tctattcctg tttatcatca taacagaaaa taaaataaca attacaaacc
     1741 cacaatattc tgtttgccac cctgtaaata taggctcaaa tctattactc gtaactgcat
     1801 caaataatat gctaattcca aaaacagtgt ttactgaaac taaaatataa gaacttatat
     1861 gctcagagca tgtcttttta tatattgaaa ccagcgaaga cattaatata tatataaata
     1921 caatatattt atatatgtct attaacttcc catatatgtt gacaaagtac atatcatatg
     1981 gaagaagaat aattggtatc actatagata tgatactcat tgtaattcct aatggtaaaa
     2041 ttaaaaatct atacacttta ttttcttcta ttttggatat acttccattt atacaaataa
     2101 tacataaaac taccataaaa tatgacaaat cttcaaccgc atatgtaaag cttaccaaag
     2161 gcagtcctaa caaatgtaca aagggatagg atacatggat ggcaaagaaa acacacatac
     2221 atccaaaata taagtaaatt ttacttttgt ttgaaagaaa ccatacagac aatgaaaata
     2281 tagctattgc aaaagttgtg aagcaaatta tactataaaa aagtaatcga taaaacatca
     2341 tattacttat gtcctttgtt tctcctaatg caggcggata atacaaacca ctataataat
     2401 gtgaaaaatt cgatacattg attataattt ctgtattttt ctttgcaata aaacttacta
     2461 ctgaattttt aattttgggt ctatattttt ctgttcctac atctcctaat ttagaaacca
     2521 tttctccatt tatccatact tcacttgcgg aaaaaatctc aggaagctct aaacttagta
     2581 gcttatcctt gccttcattt tttattctca tacgataagt agcattccca tatggtgact
     2641 gagtcccttt tctcatagaa aaatctggat attgacctat aaaaacatat tctggtttta
     2701 tatcttgctc tgtaaaatca ttcggtttaa atattttatg attataaaac tcccagtcat
     2761 ctatcaaaaa gacaagttta cctttagtga aagtatcatt atctatagtt aaaattccat
     2821 tacttgctct aatagactta tatgtatatt tattatcata tttatacaga agatttaatc
     2881 ctgttattgc cagcagagag actatcaata caatgagagt tccttttagt atattatact
     2941 ttttcattgt atcaaccaca tctctttcat agtagtatta aataaaacaa gaactattta
     3001 ttaattacag tatctcactt atggtacaaa cttatatcct tttccccata cggtttgaat
     3061 gtattgatgc tccctgctta aagaattaat tttttttctc atctgtgcaa tatgaacctg
     3121 cactgtatgt tgatctccaa tatcaggcat tttccaaact tggttatata tctctatctt
     3181 tgaaaatact tgttctttgt tctcagctag aaacaataat atttcaaatt ccatagtagt
     3241 taaatctata ctttctccat caaaagttac ttttcttgaa tttacattta ttactaacgc
     3301 tgaaaacttt aacacattag gaactgatgt aatatttttg tactgcctca tacgagcata
     3361 tattctaagt tcaagttctt ttaaactata aggttttgtt atataatcat cccccccagc
     3421 taaaaatccc cgaacttttt cttcttcatg ctcataatta gataaaaata ttataggtat
     3481 gttaaccttt tcttttatat cattgcacat tttatatcca tctatatctg gcaatgaaat
     3541 atccaaaatt atacagtcta aaactatact ctctactata tccatagcat ctttagcagt
     3601 ctctgatgta tatacattaa aacctatatt agaaaaataa atttgattta tgtttaaaat
     3661 atcaacatca tcatctacaa ccagtaaatt atacattaca ccactccatt caaaggtatt
     3721 ttaattataa ttccaagttt tatagttatt acaaattata atgttttatt ttcatatata
     3781 actggtatta ttattttata atatctttac aaactagact ataatactta agtatttttg
     3841 aataattctt gaatattgta ttaaacatta agattcatta ttaaatacta attaaagtct
     3901 ccgattgttg gagaatggag cttaaagtca ttttcacgta taaatttatt atttttctag
     3961 aaacttgtct attggctagt ttgagtattg atttatatga tttatatggt tataatataa
     4021 aataaattgt attattttgg taaatatatt gttacaaaag gtttatattc tgcatctcaa
     4081 tgtttataaa aactttattt tcttattata atactattat tcttttaaaa aaatttccct
     4141 tttccaacaa atggctactt taaaagccaa aattttacct atcaataaaa atcttaacga
     4201 ctaatactct tattttcaaa ttttgcaaca tttttgttaa ttttaaaaca aaaaaagact
     4261 atgcaatata aaaattatat tcatagccgg ttgcaccact aactccatat atctctagat
     4321 gcccaataat aagatatact tctcagtttc taaaatattt ttattaaaaa tcttttatat
     4381 attttcataa atttcttttt tattctaaca ttgtaatatt attttgtcaa tatttccata
     4441 ttcttagaga taagatacta aaatacctat tttatctaaa gatatttata tttctacatt
     4501 caataataga caataccctg caagttaaag catcattttt atgagcttat ctcacagggt
     4561 attatatgtt tactttatat tataactctt gttattgatt aactttaata aatccttttt
     4621 ctgaaacaag tgcttgtcct tcatcactta atatataatc tattaaagct ttaccttttt
     4681 ctgacaaacc atcttcttta ttaactaata taaatggtct tgatattgga tatttattag
     4741 ctattacatt tgcatcagtt gcttcaacat catttatttt taaactattt actttctcat
     4801 caacatatcc aaatgatata tatcctatag cattttcatt tccttcaact gtagttttta
     4861 tatttcctga accatcactt atttgtgcat ctttagttag ttcttctgaa tcaaacccta
     4921 caatttcttg gaatccatct ctagttcctg aaccttcttc tcttgatact attactattg
     4981 gtgcatcatt tcctcctact tctttccagt tagtaatctt tcctgtatat atacctttaa
     5041 cttggtccat tgtcagattt tttactgagt ttgcagtatt agttatcaat gctataccat
     5101 ctattgctat tttagtttcc tttaatccag cacttttttc ttcatctttt aaatctcttg
     5161 aactcattcc tatttcagat gttccttcta tagcattttt tatacctcca gaagaaccaa
     5221 tctgttgtac ttctacacta acaccttgat ttttttcttg aaacttttct ccgatagttt
     5281 ccataagagg acctattgaa gttgaacctg aaacagaaac tttgcttgaa tctcctgaat
     5341 cacctgaacc tcctgagtta gaactacatc ctactgctaa agctccaact aacattgtac
     5401 tagctaaaag aacgcctatt ttctttttaa acattttact tacccccgct tatattttaa
     5461 ttttttgttg tcatttttct atattttaat aatacagatt ctttgttaaa tgaatattag
     5521 agcagggtta agtaaatgta aatattgtta aatacatgta aaaacttaat tttatatgat
     5581 ttattcttaa ttagattaac tttttaaagt tactttaggc agatataaac ataatacaac
     5641 tcatattatc agaagtcttc attgaagcta tcaacctatc acatcctata attctttttc
     5701 tagtttatta actaattcag caaatacatt taaggcttcc tctatagatt cttttttcgt
     5761 catatctaca ccagcagatt ttaattgttc tagtgggtat tctgaccctc cactttttaa
     5821 gaagtttata tatctatcta ctgcagtact tccttctgaa agtatctgtt gacttaaagc
     5881 acttgcagca gaaaaccctg tggcatactt atatacatag aagtttgaat aaaaatgagg
     5941 tattctcgcc cattctattc ctatttgttt atctactatt gcagattttc cgtaatactt
     6001 ttcatttaat ttgtagtata tattagtaaa ttcatcagca gtaagtggtt ctccactctc
     6061 taccctttga tgtacaattt tttcaaattc agcaaacata gtttgtctat gaactgtagt
     6121 tctaaattgt tctaaataat aatttaataa gtatatagtt tcatccttag acttagaatt
     6181 ttctaataaa taatttataa gaagtaattc atttaatgta gatgcaactt ctgcaacgaa
     6241 tatcttatag tccgaatata aaaatggttg acttgttctt gaataatgac tgtgaacaga
     6301 gtgccctaat tcatgaacta aagtgaatag agaattaagg tcatttttat agctcattaa
     6361 tatataagga tgtgaatcat aacttcccca agaataagct cctccttttt taccttcatt
     6421 atcatataca tcaatccacc cttcatcaaa agcacttttt atgaccttca agtattcttc
     6481 tcctaaaggt ttaagtgcct ctaatatgat atcctgtgct ttatcatatg gtatatccat
     6541 gtcaaattta tctgtcaaag gaacatacaa atcatacatg tgtatttcat tcaatcctaa
     6601 aaatttcttt ttaagctcaa catatttatt tagattagga atacttttat ctatagctga
     6661 tataagatta ttgtaaacat ctaaacttac atcatctgaa aataaagacg cttctattgc
     6721 tgaattatac tttctagttt ttgcgtaaaa tatctctgat tttattccac cataaagcgt
     6781 tgaggcaaat gtatttttat atttctcata tgttgaaaat tcaccttcaa aagcgtcttt
     6841 tctaactcta ctattcttac tttttataaa taaagagtag tttgaatgag aaagcttaac
     6901 cttattcccc tcctcatctt ctatctcagg aaattccata tctgcatatg ctaacatatc
     6961 gtatacattt tctggaattg tcgataattc tgatgtagct gcaagtattt cttcctctcg
     7021 ttcactcaaa gtatgtggtt tatctctcaa tatatccttt atatattttg tataaaagga
     7081 tagcttctca tcttttaagt attcacttaa cttattctct tctatcgcca gtatttctgg
     7141 aactatataa gaagtagcca cacctaaatc agtagataac atttctgtct tagtggctct
     7201 tccttgatta tcattaattc tagtatcttc atgttgtttc atatgagtat atacatataa
     7261 attttgaaca atcctagatg cattttcaga gatatttaat actttatata agttctcctt
     7321 actctcagaa agcttccctt tatattcttt tacttcttgg ataagatttt ttactttact
     7381 tatgtctttt tcaatttcct ctttactgga gtacatctta tctatcttcc atttgtactt
     7441 ttcttctatt gtacttctat cagttcccat atcatacacc tctattttaa tttgttttat
     7501 attttattca ctttacaaaa tatattgtat atattttagt ccaaataatc tacatttatt
     7561 actccttcta tcatatttat ttctttcata acatcgtgta agtgtattct cttagcataa
     7621 gatacaaaaa aattaacctt ccagtattct tcattacgac attctatttc catatttctt
     7681 acgataagtt gatttttttc aaatttatca tataaaaaag gtatagagtc caaattgctt
     7741 aattttaata tcaatacatt cttttcttgg ctaaataaaa acgtattgat tatcctaagc
     7801 acaaataatg ctatcattac aaataatcca cctagaattg ccaattcata ataaccatat
     7861 ccaactgcta taccaataca tgcagttgcc cacatcccag cagctgtagt aagccctttt
     7921 accccacctt tattttttaa aattgctcct gcacctaaaa aaccaattcc agataatact
     7981 tgagcaggca ttcttgctgg gtcagcattt gtatacaaat tataatcaaa gaataattga
     8041 atcgaagtta ttgaaacaat acaagaacct attgcaatta gtatatgtgt cctaaatcct
     8101 gcaaactgtc ttattttttc tctctctaag cctattattc ctcctattat tagtgctaat
     8161 agtaacctta ttgctatctc cttacttcct atatcaatca taatataaaa ccctcctgac
     8221 ttaattacaa cttatgtcct acagataatt tacacatcag aaatactatt ttatatctta
     8281 atattattat taatatatgt aaaatatagc catctgttaa tttttgatac aaaaaaaccc
     8341 acaactgtgg gctatttgct taggtacatg tatttattta ttaatatatc tattttctta
     8401 cttgattcta ctaactcttc ttgaaatatt tctggatatt ctatgtatgt atttatctct
     8461 tccctaactg cttcaatctc attttttaac gcttctaatt cattgttcgc cataataatc
     8521 accactatta caaatttgta tatttttata tacattttat caaacaatat tatattttgt
     8581 caatattatg tcaataaaac ttactataaa aatgctttaa tttaggtatt gtatacctgg
     8641 atactaagcc tacaaacata ccagttacta atgatgccat taacataaat ggtaagtacc
     8701 ctatagcaag tatattttct atgattaaac ttgcaactat taattgacct atattatgcc
     8761 ctattgcacc tataatgctt atccctatta gtgataaatc ttttatttta ctcgctaaat
     8821 acataaataa aagactcaga taagctcctc ctatactaaa tagtagatat gataaagggc
     8881 ctgcaaatat tgatgacaaa attaccctga tagtaacaat tataacagta tctttgacac
     8941 ctaacaacat taatgaaatt aaagttataa tattgctcaa acccaatttt gcccctggaa
     9001 atataactat aagaggattt ggtatatatg tctctaatac atataaagct aaactatacc
     9061 ctatcataag acctaaaaat gtcatttttc tagttttcat taagttaact cctccaaatt
     9121 ttatctatca tcttacatta taatacaaaa tatataaaaa cacctaattt agactaaaat
     9181 atttttaatc tatttagaat aaatattagt tatttcaatg tgcaattacc tctataaatc
     9241 ttgacttttt gtatcatact tgttacaatt ttaatgataa tgaatttcat taggaggtgt
     9301 taataatgac tgtttacgat ttaaaaatag gagaaaaagg atttattaat agtatagatg
     9361 gagatattaa attatctaaa agattgcttg cacttggatg tataaatagt actgaaatag
     9421 aagttaaaaa ggttgcccca cttggagacc caattataat tagatttaga ggctttgacc
     9481 ttgcgattag aaaatctgat gctaaaaata tttctttaca ataatagagg aggagctacc
     9541 cgtgattaat gtagcattat taggaaaccc aaatgtcggt aaaactactg tttttaatct
     9601 acttacaggt tcaaatcaat atgtaggtaa ctggcctggt gttactatag aaaaaaaaga
     9661 aggatttctt ggaaaagaga taaaggttgt cgatttacct ggtatatatg caatggatac
     9721 tttctcaaat gaagaaaagg tttctaaatc atatttagaa aatgaagatg ttgatgtaat
     9781 agtaaatgta gttgatgctt ctaatttatc aagaaacctt tacttaacaa ctcaacttat
     9841 gcaatttaac aagcctatag ttatattgct taatatgtta gacattgcag aatctaaagg
     9901 agttaatata gatgccaaaa aattaagtga agaacttgga gtaattgttg ttccaattat
     9961 agcaaaaaag aaaaatggtg ttgataggat agaagaagag attaagggag ctacgcaaaa
    10021 agcttttata tatcagaaaa actttggaag tgaaacagaa acatataaaa aattagaatc
    10081 cattttatcg agatgtacaa aaactacata tacacaaaag aaatctatca gtgataagat
    10141 agacaatata gtcttgaatc ctatattagc atatccgata tttattggtg tattgttcct
    10201 cttatttaaa tttacctttg actgggtcgg aggaccacta caagagggat ttgctagtct
    10261 tatagaggct tatatttctg cacctgttag tgatatgtta tctaactcta gcccatggtt
    10321 taaatcactt atagttgatg gaataattgg aggtgttggt ggtactttac cattcttccc
    10381 tttaatattt acattattct ttggtatatc gttgtttgaa gatagtggat acatgtcaag
    10441 aacagcattt ttaatggata aagttatgag aaaggttggt ctatctggta aagcttttat
    10501 acctatagtt atgggtatgg gatgttcttc tccagctata atggcaacta gaacattgga
    10561 aagcgaaaaa gatagaagag ttacagctct tattgcacca cttatgactt gtggagcaaa
    10621 gctacctata tatgcactat ttgtagctat attctttcca cataatgctg ctttagtaac
    10681 tacatcactg tatttggtgg gtatagttat ggcaattgta gttgcattat tcttaaataa
    10741 aacagccttt aaaacagagg ctgaaccatt tatattagaa ttaccagaat atagaatccc
    10801 tactatagat gcacttatta aaaatacttg gaataaatca aaaggtttct tgataagagt
    10861 tgttactgtc atgtttgcaa tgtcagtcgt aatttgggga ctttcttcat tcaatgtttt
    10921 tggatataca gaagatataa atgtaagctt cttggctact ctaggtcata ttatatctcc
    10981 tatattcaaa cctcttggtt ttgatgattg gagaacttca gttgctattt taagtggtct
    11041 tggagcaaaa gaaatcgtag taaactcttt aaatatacta tatggtaatc taactgttgt
    11101 cttaccaaca atatttaatg gagttactgc atatacattc ttgatattta ctgctttata
    11161 tactccatgt atagctgcac ttgcaactat gaaaaaagaa tatggcaata aaatgatgtt
    11221 tacatcattt gcatatcaat ttatactagc atggataatg gcttttatag ttaaaagtat
    11281 aggtggagta ctatttatgg gtaattcaat aatggaagct ttgataggtg gaataattat
    11341 agtccttgca ctctttattc tgtttaataa attcaagtct acaaaaaacg gaaatgtctg
    11401 tagtggatgt tctatttgtt ctggatgtcc aagtgctaat tcttgctcaa gtaaggaaga
    11461 cagcaataaa aaaaataatg cacactaata tataaatatt ttagtttaaa ataagataaa
    11521 agtgggtgtc tcaaaattaa ctttttagtt catgaggcac tcttttttat ataaaatcaa
    11581 atatattttc atcatttcaa cagtaaaaaa gagcttatct agtaaaaaag aggcttatct
    11641 ctattttgag acaacctctt tttttgttac ttattcagtt tcttgctacc taacttcatg
    11701 tggaacctag tatcttttgg aacacttggg tctacacctc ttaatgtagg tatttcccca
    11761 catattagtt gtataggtac tataaaatta aatgtttgat agtaaggatt atctgtgata
    11821 tcaatcttta aatttttatc attttctgta acatctctac ctatagcaaa tacattttcc
    11881 gtccaaccag aaagtacatc aatcatctta gttactctag gttcttttcc tgtatctagt
    11941 ataaatatag tagaatcact atttatagca ttatatatac catgtataaa ctcttcaaac
    12001 tcatacccag ttacaggtat tctcattgtt tccaataatt ttaatgctgc ttctaatgta
    12061 tctccatata tatcagagtg acctattatt cttatttctt tagaattaac taacttttct
    12121 ttgtttcttt caatccattg tttagagagc ttatacactg cttcaaatct attaattgcg
    12181 tctaatatct tatttatttc ctcattgtac ttttcactag atattatacc tttttctcta
    12241 gctatttgaa gacctaataa cattaagttt aactttgtac aataatatcc ttttgtttta
    12301 gctccagatt tttcttcacc acaatttact gttaatatat aatcagaaat ctcatctatt
    12361 aaagcattct tacaacctgc catagatgct attttacagc ccttatcttc agctaacttc
    12421 atagcgttgt atgtagaata actactccca ccttgagata cacctactac tagggtattt
    12481 tcattatcaa acttgaatgt atcttcagtt atcataaatg gatacatttt cacaacatct
    12541 atatctaata aattttgtaa atatggttgc acctgcactc ctgagtgata gctagtacct
    12601 gaacctgtta ttataatctt ttttagattt gttctcttaa cttcattaaa taagctatct
    12661 gcattagata taatttctct cattctaact ggtgtttcta acatataatc ttgtattgtc
    12721 attttataca ccttccttat ctatatattc aataaattat aattaatttc tataacaaac
    12781 ctatagcatg taaagcgatt cctgttacta tagtgcctat agttaatttt gttacactta
    12841 cctttctctt taacatgtag tatattacaa atgcaaaggt taaaggtaac atattaggta
    12901 ttattttatc tagtatatct tgtaaaatta cttctgcacc atttaagttg aatttcagag
    12961 gagtagttat actcaacata gttgcaacca ttcctcctac aacgaataaa ccaacagttg
    13021 tagccataga cattattttc tccataagtc cctctctttg tattctttca agagaattta
    13081 cccctacttg ataaccatac ttaagtccaa atattctaag ggcaaatgct ggtatattgt
    13141 ataatagtaa gaataatagt ggtccaaaga tattaccttc ttttgcaagt gatactccta
    13201 ctcctgctgc tataatccta aatgttcccc agaataatga atcaccaatt cctgcaagag
    13261 gtcccataag agctgtttta acagcactaa ttgaactttc atcaaattct ggattattag
    13321 cattctcttc ttccatagcg gctgttatac ctaatatagc aggaactacc actgtcgttg
    13381 tattaaaaat ctccaaatgc ctttctattg cttttgcttg attttctttc ttattgtata
    13441 gcttctttat tgcaggtaac attgaataac agtaacctaa gttttgcatt ctttcgtaat
    13501 taaatgcacc ttgaagagca aatgaacgcc aaaacatact tcttaattct ttttttgtta
    13561 atttattcaa agtcaatctc tccttccgtt aaagtattga ttgctaattg aggattttga
    13621 ttattttctt tgttattgat ataaaagttt agtacaaatg ctacacatac tccaaataaa
    13681 gctatagcag ttatatctaa tccaccataa gatgctaaca aaaaccctat aaagaaaaat
    13741 acagacatct tttttgaaag taacatatcc ataagcaatg caaaacctac tgctggcaac
    13801 aatgttcctg caacacccaa tccgtctaat ataactttag gtattgcatt tattaatgct
    13861 gtaactttat cagcaccaat cataattgct aaaaaggttg gaactgatgt acttaaaaag
    13921 aataatatta ctggcaacca catcattatg gtaactttct taaaatcagc ctgtttagca
    13981 tagaaaactg ccttttgaga aaagtacgaa tttattattc taactaatac tccaagagat
    14041 tgtgctaaga ctgctactgg tactgctact gctaaagcaa cttctgcacc attacctgat
    14101 aatatagcaa aagctgtacc taatacacct cctgttacta catctggagg agtagctgca
    14161 cctattcctg caattcccat ccaaattaat tcaagctgtg caccaatcat aattcctgat
    14221 tttatgtctc ctaaaactaa tcctactaac atacctgtaa ctattggcct atcaagcata
    14281 gattgaccaa atatacgccc atctagtata ccaactcctg ctataatagc taacatagta
    14341 gcttgtacta acatattgaa cctccatttt aacttagttt aatttacatt aaattacatt
    14401 acatttttga ttttgttttg attttctaat aactcattta cataaacaat tccatttttt
    14461 gcttcctcta atcctgattt aactattagt tttgtatcct cctcaactaa taaaagtata
    14521 gataaaagca ttggtaaatt tactcctgtc aataattcaa atttgaaccc ttgtaaaagt
    14581 aagtttgtac atatgtttgc cacacttcca ccaaacacat ctgttaagac aacaaattct
    14641 tcactatctt tattatttga taatatactt ctaatttctt tctcagcatc atctgctgat
    14701 ttatccgttg tcatacaaaa atactcaata ttagttaatg aaccaattat taatttactt
    14761 gtgtcaacaa gctctctgga caattctcca tgtgttgcaa ttagaaattt tttcattctc
    14821 tttctcctaa attattttat taaatctgat agttgtttct ttttgtctct aggtaacatt
    14881 ctaaattcta tatcaatccc taaatcacct atttgtttaa agttatttat atcatcatca
    14941 tcaacagcaa ctaaatctgt aagtttcttc ttcccttcct catatcttaa tcctcctaca
    15001 tttatttctt ttaatactcc tcctgaattt ttagctacaa ctaaagcatc tttggtattt
    15061 tttactaaca ctaatacatt gcttttcttt gaagaagcaa atttttctac tatttctcca
    15121 gattcctcta ccccccttat atataaagtt acattgctag gtttcgctaa gttcagtgac
    15181 atagctttca tttggtcatt ttttgcttca tcatttgcaa ctagtattac atttatacct
    15241 agtgatgttg cccatccata tgcaacctga ccatgtatca atctgtcatc aattctcatc
    15301 aaagatacca ttttccccaa atcctccatc atacattaaa atttttattg tttatactct
    15361 catttatcta taaaagcaaa aagtatgcca aatactggca tactttttta aatttcattc
    15421 acttattttg taatcttgta ttttactgtg gtagaccata gataatatta taaatatatc
    15481 ctatctctga aattggtatt tgcacactat aactcttttc tagcacacta aatgcatttt
    15541 ttattagttt tacttcacta tgatggtttt tgattaacaa atccatatca gtatactctt
    15601 caatttcagt ttgtctaact aatctttcaa tcatacaact taagtgaaca tataaagaaa
    15661 tctttttatc atttgaaagt tttttctttt gaatattttc tatagcatta attccttctt
    15721 ctattctctc tattatcttt tttgaatcta aaatagttag actatctatt actctaatta
    15781 atgaaaaatt ctttacaata gaatcattta tgttctttat tttttcattt tctactactc
    15841 tgctaaatat tctaaaaaca tcatcttctc cttttcctga tattatatcc tctaataata
    15901 taaaatctac cccattcaca tttggattgg cagttcctac tatagccatc acatcataca
    15961 tttcaaaaac tggttcttta tctcccatag aaacaagact atcataatca taaggtatta
    16021 ttttgacatc aactattcca attaaacttt cggaaagtag atgttggatt tttatagcaa
    16081 tacctatccc tgtaaaacaa gatgatatta tagcatattc tttatttttt tgaggataat
    16141 acaattgtat attaggttta gtttcttcat ccataatttc tgcaatctct tcaatgtttt
    16201 taccttgcat taataaattt cctacttcta gtgcagaaat agtggatatt tggtctataa
    16261 ataacattgg gctatttatc ttattcttta aatcttcata tataagatta agagagccca
    16321 tatcaaataa tacaattaaa ccattcttta tcctatctcc ctctagatat ctagacattt
    16381 gttttgatac atcagcaata gtagcatcta taggcatatc aatagctgta aatatatttt
    16441 tacccaacat ccgattacat acattcgcca tactactggc agtagcatat ccatgtgcta
    16501 taatagtcgc attaacttga ttaaatttca ttcttataat tgttgattta ataaatataa
    16561 tcattatgat tgtatctaca ttattaagat acatatccaa tctattcgtt attaatgttg
    16621 atattttact tattatagag tattctttgt aactctcttt ttttaaatat attagtaatt
    16681 tattttctaa agctttatac tcatcagaca attgataaga ttcgtccttc aaataaagat
    16741 aagacgaaaa tgccaataca ttattcccat catattttat attgttatta acttctaaat
    16801 ttctaaatat atcctgcatt atcgatatta taagctccat aagtacgtta ttattttcat
    16861 cattatttga aaagactaaa ttgtccatta gtctaatcat tagattagaa ctctttttac
    16921 taaatttttc ctttgttatt tcattttttt ctagtctatc aaagtaatct aaacatttta
    16981 tgaaactttg atagattata tttcttttat ttcttccatt tgacatttca atactaaatt
    17041 gtttaggaga aaagataatg cttttgcttt taccactttg gaattgaaat atatctacat
    17101 tttctatata aagatttcta ggaaggtctt gaagagtaac aaaaatattt ccatttcttc
    17161 tttgattttt tgcataagct cgtccacatg catattttat tacattttta cattcaccta
    17221 tattcccctt gaattttatc tttaaaatcg catttaatgt aatttgagtt atctctatag
    17281 gcaaaccaag taccttactt tcttcataaa taaaatgttc tacaatcttt cttttttcaa
    17341 tagtccctct ttcttctata ttgggaacat aaatatttat gggtattctt cttaaaaagg
    17401 tttgtaaaaa ttcagataga ctttctgttg tagcaaatat taatcttacc ctagcttttc
    17461 taattactcc attctctcca atcctagaaa acgtacctgt atccataaat gtaaataact
    17521 tttcctgacc ttcactattt aatctatgga cttcatctaa aaataaaaca ccaccatctg
    17581 attgttctat tagacctgct ctagattcat ttgctccagt atatgctcct tttacatgcc
    17641 caaataatag actagacaat aactccaaat tattataata ctgcgcacag tttaacacat
    17701 tgtatggaga atttttttct aatacattac tgcttacact atatttgtat attatcttgg
    17761 ctaaaaaagt ttttcctact ccactttcac ctgtcagcat tattgtaagt ccgccatttg
    17821 gataaagtac agcggttttt atttgttcga ttacttcttt taagctatta tcatatccta
    17881 tgatactatt aaacacagtc tcttgcttta ataatttaca ctcattttta agttcatcta
    17941 aattttcata aatacttttt tttgtaggtc ctactacatc atttatttta tttttaggta
    18001 aaaaaataac aggtctactg tttatcttta taagattatc agattttact aaatcattta
    18061 ataatctact tgattgagtt ctacttaaat taaacttttt tactatatct tctgttatta
    18121 tgccactttc ttttttaagt tcatcaaagc tgttaaataa tacacattca tttacaaaac
    18181 ttaaaatttc atccctcatg ttcaacctcc aatacaaata tcaataatac tataatatta
    18241 gtatatcatt tatatattta tgaaaaagat aattatattc ctatcttgat tctcttttgt
    18301 aaggttgtaa aatcaaactt tttataagta taaaatctag attttttaac ataatctcaa
    18361 ggttcttatc tttccatata tctgacatag aatttat
//
LOCUS       DAMKMI010000079        18373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMKMI010000079 DAMKMI010000000
VERSION     DAMKMI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.080"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            177..833
                     /locus_tag="PC429_002373"
     CDS             177..833
                     /locus_tag="PC429_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847874.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="PC429_002374"
     CDS             complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="PC429_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF3847875.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2857..3573
                     /locus_tag="PC429_002375"
     CDS             2857..3573
                     /locus_tag="PC429_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF3847876.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3652..4962
                     /locus_tag="PC429_002376"
     CDS             3652..4962
                     /locus_tag="PC429_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF3847877.1"
                     /translation="MTELPNEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5062..5598
                     /gene="lepB"
                     /locus_tag="PC429_002377"
     CDS             5062..5598
                     /gene="lepB"
                     /locus_tag="PC429_002377"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF3847878.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5764..6300
                     /gene="lepB"
                     /locus_tag="PC429_002378"
     CDS             5764..6300
                     /gene="lepB"
                     /locus_tag="PC429_002378"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF3847879.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6310..7065
                     /locus_tag="PC429_002379"
     CDS             6310..7065
                     /locus_tag="PC429_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF3847880.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7139..8806
                     /locus_tag="PC429_002380"
     CDS             7139..8806
                     /locus_tag="PC429_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF3847881.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9347..10276
                     /gene="glsA"
                     /locus_tag="PC429_002381"
     CDS             9347..10276
                     /gene="glsA"
                     /locus_tag="PC429_002381"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF3847882.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10374..10937
                     /locus_tag="PC429_002382"
     CDS             10374..10937
                     /locus_tag="PC429_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847883.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11425..12261
                     /locus_tag="PC429_002383"
     CDS             11425..12261
                     /locus_tag="PC429_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF3847884.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            12545..13540
                     /locus_tag="PC429_002384"
     CDS             12545..13540
                     /locus_tag="PC429_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF3847885.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(13797..14201)
                     /locus_tag="PC429_002385"
     CDS             complement(13797..14201)
                     /locus_tag="PC429_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF3847886.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15042..15956
                     /gene="queG"
                     /locus_tag="PC429_002386"
     CDS             15042..15956
                     /gene="queG"
                     /locus_tag="PC429_002386"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF3847887.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16062..18299
                     /locus_tag="PC429_002387"
     CDS             16062..18299
                     /locus_tag="PC429_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3847888.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
BASE COUNT         7166 a         1784 c         3419 g         6004 t
ORIGIN      
        1 gataatggtg gatagagtag taagaaaata aatagataaa gggatagaat aaatttaaat
       61 atggaaaatg aatatctaat aataagtgat taataaataa ttgtagatag attgtaaatt
      121 aaataaatct ttaatagaaa ggagaactat ttatatatta aatagtattt ataaatatga
      181 aaaaaggcga ttttatatgg agtggagttc ttatttgtat tattgctgtc ttagcaatac
      241 caagtagtag agaagtattt atggaagcaa caggtgcaca tccatatatt ggaggttttt
      301 taaagtttgc tgtattagct acaatgggag atttacttgg tgctagaatt ttgtataagg
      361 attggaaaat accagtagga gtattttata gagcaatagt ttggggaata ataggtgtag
      421 ttaccacata tggaatgtca atctactcat caggtgtagg tcaagcacaa gtggatggta
      481 tgcttccatt tgagggaata gcttttgcaa atgctctatt taagagtgca actatgaata
      541 tactaatgtc accaactgta tttttattcc ataaggttat ggactgtttt atagataaaa
      601 aatatgaggt tggaaagggg attaaggtaa aacttaaaga tgtggtaaat attgtggact
      661 ggaatgctta ccttggtttt agtgttttaa aaactatacc atttttctgg ataccatgtc
      721 atacaattgt attcttattc cctgctgagt atagagtaat tgcttctgca tttgcatcta
      781 tagctttagg tctaatattg gctttagcta ataagtctaa agttgcagtt taggttcact
      841 gatgaagata gtagtattat ttaaattagg aataactttt taaattaata ttatttgtat
      901 ttataataaa taataattta tataagaagt aatattctaa gtgatggata gtgttttaaa
      961 taataagaag taatactcta agtgatagat agtgttttaa ataataagga gtaatactct
     1021 aagtgagaag tagcatttta aataataagt gagattttaa ataaaaagta ggtttttcgt
     1081 tagaatagca ttttattaaa cttaaaaata aatataatat tttaaagaaa tctaagttgc
     1141 taagcactgt ttttaagaag cttaaaatcc cccatttaaa gttttaagat tctaaaaaaa
     1201 caagttataa ctaaaaaatg gctgttaaga ctacaatact tataagtaat acttatggta
     1261 tatattagtt cataacagcc atttttattt taaattaata attttattca ataatttgag
     1321 tttatgttct aaatcaagct ttaaattaaa ggatttaaag aagctattct agttgtagct
     1381 acataaactc tggatatttc ataccatgtt ttaaaatcta ctactccgct ttgaggtaaa
     1441 ccaaagattc cttggaatat cttaacagcg ttttctgtat ctgttccata gataccatct
     1501 tctataactt ttggtacagc tggataagaa ttagatatag catttaactg attttgtatg
     1561 gttctaacat actgtccaga agaaccaact tgtagtgtag taccagggaa tgacactggt
     1621 acaccactta ctataggcgc tctttcaaat actatctcat ctccatagaa atatcttagt
     1681 atactctcat agtcatatcc ttggtcacct aagtctttac tgccccattg agtcatttgg
     1741 tcaggacatt gggatttttg tccatcacaa tattgtgcta atagtggctg tcttgatgta
     1801 ggaggtcttt ttataaaagt attaaaaata gcatctacaa ctacgttaat tggttcaaat
     1861 aagtttctat tatttataaa cttatggtcg tatgctgttg ttgaagttat tgtaaagtta
     1921 tatcctttat ttctatacca ttcagtaaag actctattta aagtaaatga tattatagct
     1981 attacatttg cataaattgt ctgttcaggc caagttgaat atatttcaga tgatgctatg
     2041 tttttaatgt actctttaaa tggaatccaa tagtttggag ctgatgaatc ttctggcaaa
     2101 ccatcatgaa ctactataaa ttcaggcact acagggttat ctaatacaac gaaaccagta
     2161 ggaggtggta atggttttaa gttactttct ggaatcttag gaggataagt accatataga
     2221 gtatgttctc ctatgtcaaa aatcaactca ctttgtcttg agaaagaacg cttacttctt
     2281 gttctaggag acatgggaac tccttgtctt gcttcaattg tagctaacaa ctgtgttcct
     2341 tctatgacaa ctgtctcata tccatctgct atggcttcca ctatatactg gctataaggt
     2401 ctaacatcag atggttgtag agagtaatct acatctggtg ctggtaatac taaaccagtc
     2461 acttgaccag attcattact tcttaggttt tggtataaag ttgatgagct ttggctacca
     2521 ttagacattg aatatatgtt tactactgca ttttgaatag gtctattgtt agtagcatca
     2581 attatgctta ctgttaagaa accatcttgc atcaaatcac cctttcttta aatgaattta
     2641 gttcccttat atttcaagat atgaatatag cttaaaattt gttaaataat atagatataa
     2701 atttaaattt ttttgagtat ttgagtatat ttgtatttaa ataatttaaa ataatttaat
     2761 gtatatttta gtatgtattt aaaacacgaa taagttaaaa ataagtagat gatataaatc
     2821 ataaataaat caaaaatttt gaaaggaaaa taatctatga agctagattt atttttatta
     2881 gcaatagttc ctatattgat aggaatgttt tggataaggt ctaaagatag atattgtaga
     2941 gaacccctaa tccatctaat taaatttttt ttaataggtg cttttctaag tgttataatt
     3001 atacttttag aaaatttact gatgaagttt aatgtatttg aagggtattc agaactaata
     3061 tatgtatcat ttgtagttgc aggacttgta gaagaaggtg taaaagctct gatactgatt
     3121 cctgccctaa ttaaagaaaa acactttact gaaaagttag atggtattat ttactctgta
     3181 tttttagctt taggatttgc aacaattgaa aatatggttt atattttttc tgaaagtaga
     3241 aatctagcac tgcaagttgg tattaatagg gcagttatct ctataccagc tcatgtaatg
     3301 tttgcaatta ctatgggcta ctatatttct aagtataaat ttgagggaaa taaaaataaa
     3361 agacgtgagt atttgtttat ggcagtgtta attccaattt tattacatgg agtttttgat
     3421 ttcatattga tgatagaata tagatgggct attatattac ttatagttta tgtaataatt
     3481 ttgtggaaga taaatcttga taaacttgag aaatatatga atcactctaa aaaagtgttt
     3541 ttcggtaatc ttaggaaaaa gaagaagaaa taaaatttaa aggaaagaca taaataatta
     3601 aagtataata aatgacacac aagtaaagca gattgaaggg ggattataga tttgacagaa
     3661 ttaccaaatg aatttttaga agatattgaa gaaatactaa aagatgaata tgatgatttt
     3721 ataaaaagtt atgaggaaag taagactact gggcttagaa taaatacttt aaaaattgac
     3781 aagaaaaaat tatttgattt ggagttgttt gatttgaatc aaataccatg ggcagaagaa
     3841 ggtttttatt atgatgataa aattgacaga cctggtagaa atccactgca tgaggctgga
     3901 gcatactatt tgcaagagcc ttctgcaatg agtgtaatac caaaggcaga tattaaagag
     3961 ggtgaaaaag ttctcgatat gtgtgctgct cctggtggaa aatctacata tatactatct
     4021 aagctaaacg atacaggttt attggtttct aatgaaatta atgctagtag aattaaggct
     4081 ctaggggaaa atttagagcg atttggagcg agaaattgta ttataacaaa caccgattct
     4141 aatagcttaa gaaagacgtt taatgggtat tttgataagg ttattataga tgcaccttgt
     4201 tctgggcaag gtatgtttag gaaggatgag gttgccatat cagattggag ttatgcaaag
     4261 gtagtagaat gtcaatccat ccaaaaagag attataagag atggatataa aatgttaaag
     4321 aaggatggca ttttaattta ctcaacttgt actttttcaa gggaggaaaa tgagcatgtt
     4381 ataaatgagt ttatcagtga atatccacat gcagaactta ttgaaatgga aagattatgg
     4441 cctcataaga taaagggaga aggtcatttt gttgccaaaa taaaaaaatt agaagaagaa
     4501 gaatgtaatg tcaaagaatt aaaaataaaa aatttagata aagaaataaa ggattatagg
     4561 gagtttgaaa agaaattttt aaaggttgat ttttatagta ataaaaaaaa taaattttat
     4621 ttaagaggag aaaatttgta cctacttcct gaaatatatc cagatacgaa aaaactaaaa
     4681 gtattgagat atggtttgca tttaggggtt ttaaagaaga atagatttga gccttctcat
     4741 gcactatctc attacttaaa agttgaagat gtaaaaaatg ttgaaaactt cagtgtacag
     4801 agtgaatcaa ttcttaaata tttaaaaggg gatgttgtaa attctaatga tagtagagga
     4861 tgggttttgg tttcggttga aggaatacct ttaggttggg gaaaagaatc ttctggtgta
     4921 attaagaatc attatcctaa aggtcttaga aaagtatttt aaactattag aaaaaagaag
     4981 tataatattt atattgtagg tttatggtag attattttgc tgtagattga cttaatattt
     5041 aacacagagg agacgatttt agtgggtgaa gcagttaaaa aagaagttgt agaatggata
     5101 aaagtgattg tcatagctct tgttttggca tttgcaataa ctcgttttat agtgccaaca
     5161 atagtcaaag gagaatcaat gtatcctaca ttagttgaac gtgattattt gatagttaac
     5221 agaattgcgt acaaggtagg agagccaaaa tacaaagata taatagtatt caaaaccgac
     5281 ttaacagagg aaaatggaaa gaaaaaagat ttagtaaaaa gagttatagg tgttcctggt
     5341 gaccatgtaa aaatacaaga ctccaaggta tatgtaaatg ataagttgtt agatgagact
     5401 tcctatatac ataataatcg tactgatgga gatattgata tcgtagttcc agaaggaaaa
     5461 ttatttgcaa tgggagataa tagagaaaaa agtttagata gtagatacga tgaggttgga
     5521 ttggtcgacg agcataccat tttaggaaag gttctagtca gattgtatcc attttctaag
     5581 ataggaacta ttgactaaac atataaaatt tggagcaatc gctgattagc tgaaatcacc
     5641 agaccataca caatagttgt ctatataaaa aagacaacac cttatttagt agacttatct
     5701 aaaagatagt gtttaagaga cattatcaat aaagataata taactataaa ggagaatttg
     5761 tgaatgaatg aaactattaa agaagagatt gtagagtgga taaaaataat tattactgca
     5821 cttttttttg catttattat aactcgtttt ataaaaccaa cattagtaaa tggagaatca
     5881 atgtacccaa cacttaaatc acatgattat ttggtagcaa acaggatgac atataagtta
     5941 tcagaaccaa aatgtggaga tataatgata tttaagactg atttattaca agagaatgga
     6001 aggaaaaaag agcttgtaaa aagggttata ggtgttcctg gtgaccatct aaaaattaag
     6061 gattctaagg tttatataaa tggtaagtta ttaaatgaag tttcatatat acatgataat
     6121 tatactgaag gcgatattga tatggtgatt cctaagggaa aagtatttgc gatgggagac
     6181 aatagagaag ttagtttaga cagtagatat aaagaagtgg gattagtaga tgaagaaaat
     6241 attaaaggaa aagttatttt aagagtattt ccttttacag atataggtat ttttgagtag
     6301 gtgatttgta tggcagttgg tatagcgtcc ttattgttta atattgagga ggctttaaac
     6361 atttgtgaga gtataaagca aataactcat ttagagattg gaattgacaa tatatctgaa
     6421 tgtagtgagt tatgtaaata taaagagaga atttctaagt taggattatc tatagggata
     6481 cacttgccta tggaattaaa tacttgcgaa aacatagagt atatacgaaa ttcatggata
     6541 aattttattg agaagataga atttgaacta aaaggatttg atttaaggta ttttaacctt
     6601 catttgggat atgttatgac aaatagagtt agaaagaata gagacaaata tttaggaaat
     6661 agtgtggatt ttttagataa attaaataca aattcatatg tatgtataga gaatacttat
     6721 tcaaaaggtg gggatttttc aaatattgga aatatatctt atgattttga atatatattt
     6781 aagaggatta aaaattctaa gatttgtttt tgttacgata ctggacactg tttaattgat
     6841 gaggatgctt atgtaaagaa cttaaaggat aaaataagac tgatacactt aagtgataat
     6901 gatggtatca atgatacaca tgttggtata ggaagaggaa ttttatctga agaggggatt
     6961 aaagaggtct taacattaga tgcagaatat ttagtattag aaataaatta cgaagacatt
     7021 gaagatacta taagtaaatt gaataatatt gtaggagagg gttaaatacc tctctttatc
     7081 ttgttgatat gcctcattaa ttaaaattta tattcatcaa aaggaaggag atgttgaagt
     7141 gaaaaaaata aacttaaaaa tagaaggaaa agataaggaa tattcattag aagaaaattc
     7201 ccctggaatc agattgggag atatagcaaa agaattttgt gatgagcata agggttatat
     7261 aacacttgca gttgtagaca ataaactaaa agagctaaat tgtagagtta agaaggattg
     7321 tgagataaat tttttagata ctactaatga agatggagag agagtttact ttagagtcat
     7381 gtcatttata tttgtaatgg catgtagaga aatcttttgg gatagcagag ttacaataga
     7441 acattcacta tcagatgggc tatattgtga agttcatata gatagaaagc ttaaagaagc
     7501 agatgttgaa aatataaaaa acaagatgaa agagatagta aacaacgatt atgttataga
     7561 aaaaattgaa gttacaagaa atgaagctat ttctatcttt gaaaataatg aaatgtatga
     7621 aaaggcagag cttttgaaat acaaagagta tgatagtgct aaagtatata agtgtagaaa
     7681 ctatatagac catttttatg gttatatgtt gccatcaact ggttatataa aatcatttga
     7741 tattaagctt tataatggag gcgttataat tttaggacct agtgaagaag ataagactct
     7801 tcctatgaaa tttgtacctc aacctaagct atcaagtgta tattatgaag cagaagaatg
     7861 gtcaagactt atgggaattg acaaggtagt atctcttaat aaaataatag aaaacaatga
     7921 atatggagat ataataagga catttgaagc attgcatgaa aagaagctat ctcaaatagc
     7981 ggatatgata aaggatggca ataagagagt agttttaata gcagctccat cttcatctgg
     8041 aaaaacaagt tttgctcaca gattatcaat acatcttaga gtaaataatt taaatcctat
     8101 ttcaatttct ttagatgatt attttataga tagaaaacat actcctttag atgaatatgg
     8161 aaattatgat tttgaatcaa tttatgcaat agatttagag aagtttaact tagacttaaa
     8221 gaaactttta gctggtgaag aaattgatga tataagattc aattttaaag aaggtaagag
     8281 agaatacact ggtaaaaaga taaagataga ttcaaatcag ccaattatat tagaaggtat
     8341 acatgggcta aatcctattt taacatcatc tataccagat gaagataaat ttaagatata
     8401 cataagtgcc cttactcaaa taaatttgga tgaccataat cgaatcccta ctgctgattt
     8461 gagaatgata agaagaattg ttagagacta taattttaga ggatatagtg ctgataatac
     8521 tattttacaa tgggcatcag ttagaagagg agaaaagaag aatatattcc cgtaccaaga
     8581 ggaggcagat gctatattta actctgcttg tgtatatgaa ttggcagttt taaaacgatt
     8641 tgcgaaacct ttgttggaag agataaagga agataatcct gcacatattg aagctactag
     8701 attactaaag tttttgcagt attttgttga attggacgat acttctgaca taccaggtat
     8761 ttcaattctt agggaattta ttggaggaag taaaatagtt gattaatgtg tgtattatag
     8821 attgttttat gaagattaaa tggcatagca agtagtgtta ttggtttatg ttaagtatat
     8881 taagtgtaaa gtggagaatt aaattgatac agattttaaa gtttgagttg atatataaaa
     8941 aggcttgttt tatacttttg tatgaagcaa gctttttatt ttaaatttta cttttttgtt
     9001 taatattttg ttagtttatg tgattatttt ttaaaagagt aaaagagtaa aagagtaaaa
     9061 gagtaaaaga gtaaaagagt aaaagagtaa aaggtgttgg gttagaaatt tttggtgtta
     9121 taaaaattta aattactgga ttattagatt gctggattgc taaattacta ggttgttaaa
     9181 ttattgaatt actaaattat taagctcttt aaactattta aattgtttga aaaatgaatt
     9241 gattcttgta gaaaataatt ttaaagtata atttttttta acattgtttt attatagtat
     9301 aaacttgtgc taaaatagaa tatataacat aatataagag gtgcatatga atacgttaga
     9361 tgagacatta ttaaaagaaa ttataagttc taataagaag tatactaatt atggacaagt
     9421 cgctagttac atcccagaat taaaaaacgc tagaagaaat gatttaggaa tttgtatcat
     9481 agatagcgaa aataatcttt atagtgctgg aaactgtagc acaaagttta ctatacaaag
     9541 tatttcaaaa ccaattgtgc ttgctatggc acttatggat aatgattggg aagacgtttt
     9601 ttcaaacgtt ggaatggagc caagtgggga cccatttaat tcaataatga aacttgaaat
     9661 taatgacaca aaaaaaccct gtaatccaat gataaatgca ggggctatag taaccacttc
     9721 ccttataaat gggagttgtt tagaagaaaa agaagaaaga atgcttagtt tctttagaaa
     9781 gttagcaaaa aatgataata taggaataaa ctatgatgtc tataagtctg aaaaaatgac
     9841 tggagataga aacagagcta tggcttactt attgaaaagt gatggattta tcagagggaa
     9901 tgtagaagat gttctagacc tttattttaa acaatgctca attgagatag attctgtaga
     9961 tttagcgaga attggaataa atcttgcaaa ttatggagta gatatagaaa atggtgagca
    10021 tttaatgagt gaaatggttt ctaggattgt caagacattt atgatgactt gtggaatgta
    10081 tgatgcttct ggggaatttg ctattaaagt tggcattcct gcaaaatctg gagttggggg
    10141 tggtataatg gcttctgtgc cgggacgcat ggggattggt gtttatggac ctgcgcttga
    10201 caaaaaaggt aacagcgtcg caggagttaa agttttagag gagttgtcaa ataaactcaa
    10261 attaaatatt ttttaaaata ttaagaaagg atttttttga caacaacata taatttaata
    10321 tataattaaa ttgtaaagaa atatgaatta aataaatttt ttaggagggg tcaatgaaag
    10381 aggtattggt attacgagat ttggagtgca taaaggctat tgcgcatcca cgtagaatag
    10441 atattctaaa agcatttgac aaatcgccat tgtcagcaaa acaattatct caattattgg
    10501 aagaacctca tgcaaaaata aactaccata taaaaatgtt atacaaagtt ggtattttag
    10561 aattagttga ggaaaaaata aagtcgggga ttgtggaaaa gtactattat ccaagtgcaa
    10621 aaaatgtggt tatagggaat aggatactta atttttcttt agacaatggg gaagaaaaag
    10681 aagaattgta catatcaaaa tttgaaaata tgagtgaagt tttttataag gcagcagagg
    10741 aagatgtttt agaaaatgaa aatatagttg attatcatga tatttctctt actcatgatg
    10801 aattagttga acttagtgac actatgaagt caaagataga tgaaatttta aataaaagac
    10861 aacacaatgt tgaaggttct aagtatgata tagctatggt tatagttcct actcttgagg
    10921 aggaatgtcc tagttagaga attaattatg gattaattaa agatataata ttagagattg
    10981 aacatcaaaa tttacttaat aatcaaaaaa tagtttttgg tatgggtaaa acaatgattt
    11041 ttaatctcta tttttttgtt tttttatacc tattgtattt gtaaataata tgtatagtgt
    11101 aagatgtata cactatctaa gaagtgctta gataatatac aaagtatgta agattataat
    11161 atttgaaatt gattaaaatg ggtataatta aagtgtaaat attaaaatta aaaattaata
    11221 taaattaaat caatgctaga aatatgttaa aagaggactg agattagttg ggatagaaca
    11281 tcccttagag cttacgaatt tttagtgaaa aagtactatc aaataggaat ctccatcttc
    11341 atatgttggc taaatgtagt taaggtggag tagttcaaaa ttaaaattga gtacaagtag
    11401 tagtacaaat aaaaggagat taatatgatt aaattaggaa cacatctttc aatagcaaaa
    11461 ggatttgcta atgcggctga gacagctgta tatattggtg caaatacatt tcaatttttt
    11521 agtagaaatc caagaggagg aaatgctaaa gaatttgatg aaaaagatat aattaaattt
    11581 caagaaatta gaaaagaaaa taattttgca ccactgcttg cacatgcacc atatactatg
    11641 aatctgggtg gtacaaagga tgatgtatac gaatttgcaa caagggtgat agatgaggat
    11701 ataagaagga tggactcatt aggaattgaa tacatgtgtt ttcatcctgg aagtcatgta
    11761 ggtggtggtg tagattttgg catagataga atagtaaaag gattgaacaa tgccataaag
    11821 ggtgatgaaa atataactat acttcttgaa actatgtcgg gaaagggaac tgaaataggt
    11881 tttaaatttg agcaattaaa aagtataata gacaaaattg agcataaaga aaaaataggt
    11941 gtgtgtcttg atacatgtca tattttttca gctggttatg atatagtgaa tgatttagat
    12001 ggggttttgg aagaatttga taaggttata ggcatagaaa gattgaaaac tgtccatcta
    12061 aatgatagta tgatgccatt tggagataaa aaagacagac atgctcctat aggggaagga
    12121 aagataggat taaaagcatt aattgatttt atggaacatc ctagtctaaa acatttacca
    12181 tttttcttag aaactccttt tgatgatgaa ggtcacaaga aagagcttaa aatgataaaa
    12241 gaaatactat ctagtaaata aacctttatt aaaaatatgt gaaatacttc accctcaaca
    12301 taattagggt ggagatacaa tcatgaaatg tgataattta aattaatgga taggaataac
    12361 aaaaattgta tacaaattaa ctgggtataa agtatgatta tattatgaat agactgatat
    12421 aaaatataat tatactatga atagtatgta aatatattgc aaagattctc ataatttgtt
    12481 gtataattaa agatgacata tttatattat ataaaaaatt attaaaagtt aggtggggaa
    12541 agatatgaga aatttaggca atactaacat gaaaataaag agagttggct ttgggggcat
    12601 tccaatacaa aggattactc aagatgatac taacttagtt ataaatgagt tagaaaaaca
    12661 aggaataaac tttattgata gtgcaagagg gtatacaata agtgaggaag caatcggaat
    12721 tgccattgaa ggaaaaagag ataaattttt cttagctact aaaagtatgt ccagagatta
    12781 tgattcaatg aagagagatg ttgaaataag tttaaataat tttaaaactg attttataga
    12841 tttatatcaa ttccataatg ttaaagaaga ggagtatgat aatcttttta aagataaaat
    12901 ggcttatagt gcccttttag aggcaaagga gcaaggtaag ataaaacata ttggtataac
    12961 tagtcacaat ttaaatacta tagaaaaagc tatagaagat ggaaaatttg acactataca
    13021 attcccttac aatatagttg aaggacaagc agatgaagtg tttaaaaaag ctcatgaaaa
    13081 aggaattggg ataatagtaa tgaaaccttt agctggaggt gctttagaca atgcaacact
    13141 ggctattaaa tatatactat ctaaagatta catagatgta gttataccag gtatggagag
    13201 tgttgaacag gttagacaaa atgtcgctgt acttgaaaat cttgtgctag atgaaaaaga
    13261 taataaggaa attgaagaaa taagaaattc tttaggtaaa aaattctgta gaagatgcga
    13321 atattgtatg ccttgtgctg taggaataaa tataccattg agttttttat gtgaaggtta
    13381 ttatactaga tatggattaa aagagtgggc taaagaaaaa tatgaggtta tggatgttaa
    13441 acctactgag tgtattgact gtgggctttg cgagagtaga tgtccatatg aacttcctat
    13501 aagagaaatg cttaagactg ttgttgaaaa gctaggatag attttattta ttgaaatcgg
    13561 tgtattaaat ttactccgat ttctttttat tagatgtggt cttatataca aatattaaat
    13621 ttacatagtt atagctatta tataaattta atatttgttt aatacttatt aaatatttac
    13681 ttaatgcttt ttaaaaacat ttgtttgagt atagacataa cacaattatt attgttgtat
    13741 tagtatagtt tcaaatactg gagtatcaat ataatgtcga ataacacata aaatctttat
    13801 ttttcaaaaa tattatagga tatatcgtat ttagatactg gataaagttc aatttcaacc
    13861 ctagggttat ctttatctat aaaatcaaat aaaagtactg gcttaagttg tttatcattt
    13921 tttattattc cacttttttc aataccatca caaatacttt ttggataatt atgtaaatcc
    13981 cccattctct tatttgggaa gaataatttc aagactgtta ttagattttc ttcaacagtt
    14041 tccccttgat actgttggtt tataaaacct gcaatcatgt tttcgtatgc aagatactta
    14101 gaatattttc cctttgaagg catccatgct tgaccattta tgttatgaag cttaaaatta
    14161 gatttactaa taggtctacc tggaattgtt attttaatca aatttttcac catccttata
    14221 cagtatttat tataatctgt atatgattta aaagcaaatt gttatttaaa gtgtttttac
    14281 agagcttgtt tgtaaaattt tccactttag atgtagtaat ataagataag tattttgctt
    14341 acaaatttta aaatttgata tatgatgggc atattcatat tagactatta taagaccttc
    14401 ataatattaa taagtataag tttagtaaag agagtaaaaa gaaatccata taagtcaagt
    14461 aaagacttaa taaatggcaa tgtaaatggg atattaatta taagaaaaaa tcaatctttt
    14521 tgcttaacta tagaaagaat gtcaaaatat aactaatgat atttgaagtt ttagagttta
    14581 tattaaaagg taagaaggtt aagtgggtaa aaaaggttga atagattaaa aaggctaagt
    14641 atctaaaaga gttaagtagg taaaaaagtt aaataagtaa atgaaaaagg ctaagtatct
    14701 aaaagagtta gtaggtaaaa aagttaaata agtaaataaa aaagatgaag tatctaaaag
    14761 agttagtagg taaaaaacta aataggtaaa aagatgaagt atctaaaaga gttagtaggt
    14821 aaaaaattaa ataggtaaaa agacgaagta cctaaaagag ttagtaggta aaaaaattaa
    14881 atagataaaa agacgaagta tctaaaagag ttaagcaggt aagaaaattg aatagctaaa
    14941 aagattaagt atttaaaaga gttagtagct agaaaaggtt aaaaagacta aaaaaattaa
    15001 ataaatttaa aaagtcaatt agattaaaaa ggaataattt tatggataat aaggaattaa
    15061 aagcattttg taatttaata ggattaaact gtgtaggagt agcaggggta gataaatatg
    15121 ataatttaga aaagatactt aagaacagac aacaaaaagg ttatttaact ggtatggaag
    15181 aaccaataat tgagagcaga attaacccaa gacatataat gaaggatgct aactctataa
    15241 tagtctgtgc atttccatat catataaata gacaagaaaa gaataaaaat tcaaatttat
    15301 caaaatattg tcatggaaag gattatcaca ttgtagtaaa ggacttttta caacaaattt
    15361 gtgattatat ttctaaaaca gtaacagatt ttaaatacaa gttatttgct gataatggac
    15421 cattagttga caggtattta gcttatttat caggaatagg atattttgga ataaataata
    15481 atatcataac tgatgaatat ggttcttacg tatttatagg gtatatagta aataattata
    15541 aatttgaaga agataaacca ctagaaaaaa gttgttttaa atgtggtaag tgtgtaaaat
    15601 attgtccggg aaatgctata ttgggaaatt atgatatgaa ccccaaaaaa tgtttatcat
    15661 atataactca aaaaaaagga gatttgtcag agaaagaaaa agttgtttta aagaatggca
    15721 aaaaggtatt tggatgcgat atttgtcaag aagtatgtcc acataatgtt gaaataccaa
    15781 caactcatat tttggaattt aaagaaaata taatagatta tttggattat gatgagatac
    15841 aaaatatttc aaataaagaa tttaaaagga gatatggaga tagagctttt agctggagag
    15901 gtagaaatat aataaagaga aatatggaaa taattttaga taagtatgat gactaataat
    15961 aagagtctgt ttcatatact ttaatgtgta tttactttaa cacttgttgt gtaaaagtca
    16021 tattatatca ttaaagttaa atatggggag gtgggggaat attgaaatat aataatacat
    16081 ctgaaataaa tccactagat tatatattgg gtcaagaaag agctgtagaa gctatggaat
    16141 tagggttgaa aataaataat ccagcgtaca acatatatat agctggtgaa cctggaactg
    16201 gtaaaagtac ttatgcctta aaggttttaa atgagtatgc atccaaaaaa aatacgcata
    16261 aagattggtg ttatatatat aattttgaaa accctagaga gcctattata gttgaattag
    16321 aaaaggggtt tggaagagaa cttaagaaag atatggagaa attaattgaa agtcttttag
    16381 aagattttaa aaatgcgttt gaaagtgaaa attttgaaat agaaaaaaat aaacttctgg
    16441 atgaatatga aatagaaaaa gatatgcttt tgaagcaaat taaaaaatat ggagaagaaa
    16501 aaggatttaa attaaaacag agtaaaatgg gaatagtttt tatccctttg aaagaagagg
    16561 aagatgaaag tgatgagtct gatgaagagt tttacaaagc taaaagagaa ctagaaaata
    16621 tggcgataca ggtcgtatat aaaattagaa acttagaaga aatagctgaa aaagcagtgt
    16681 tagagcttga agaagagata gctaaacttg tagtagaacc tcatatcaag agattgtgtg
    16741 aaaaatatga aaattatgat aagattcaaa cttatctgga aaatataaag aaagatataa
    16801 tagaatatat gtatttattc tatttagatg aagaggagtt aaaagataag tatgataaag
    16861 agcattttat taaatataaa attaacctat ttgtagacaa tggaagctca aagaataaag
    16921 aatcagcacc tgttgtagta gagataaacc caagtccagc aaatctattt ggaaaggcag
    16981 agtatgatta tgcaaatgga aatataaaga ctgatttcac aaaactatta tcaggagcat
    17041 ttcatagggc taatggtggt tatttagtac tttatgcaga ccaattacta aaatatacaa
    17101 tgtcttggga aattcttaaa aagactttgc agactaaaaa agtaatattg gaaacacaaa
    17161 cagcaattaa accagaaaat atgccattgg atgttaaatt agtactgatt ggaagccact
    17221 atatatatga tattttatat agatatgatg aagattttga aaaatatttt aaagtatttg
    17281 tagactttga tagtgaaatg gataaaaatg aagataatga agaaggaata gcaagattta
    17341 tagcatacca atgtgataag aataatttaa ggcattttac taaatcagca gttgaagagg
    17401 ttattaaatt tagcactaga ttatctggtg atttagaaaa attatcaact aagtttaata
    17461 aaataatgga agttgtaata gaaggaagtg cttatgcaga gtttagaaat tcggagtata
    17521 caaagcagtg tgatgttaaa aaagcaataa gtgaaaagag aaaaagaatc aatagagtgg
    17581 aaacacatat ggatgaatca attgaaaatg gatttactct aatagaaaca gagggaagaa
    17641 aagtaggagt gataaatggt ttgtcagtct taagtacagg agaatactct tttggtagag
    17701 cttcaaggat tacagcgacc actagcccag gaagtaaagg catagtaaat atagaaagag
    17761 aagtaaatat gagtggctct atacataaca aaggagtact tatattaggt ggctatcttt
    17821 ctgagaattt tgcacaggat ttactattgt cactaaatgc atatgtttgt tttgaacaaa
    17881 attatggtgg aatagatgga gatagtgcat cttctgcaga attatatgta ctattatcat
    17941 ctcttagtgg agtatcaatt aaacaaaata ttgctgttac aggttctata aatcaaaagg
    18001 gtgatataca agtagttggt ggtataagtg aaaagataga aggattttat tctatatgta
    18061 aaactaaagg gttaaatggt gagcaaggcg tcataattcc tagaaaaaat caaagaaatc
    18121 tcgttttatc agatgaagtt aatgatgcag tgcgagatgg aaaatttaag atatatactg
    18181 ttgaaagagt agaagaggct atagagatat tgacagatgt taaatttgaa gaaataaaga
    18241 aattggtaaa agaaaaatta atatctttca gcaagataca aactgtgtca aaagaataag
    18301 tttattatag aatttaatgg gtatttatgt ataataaaaa gcaagaactt taataggttt
    18361 ttgctttttt tgt
//
LOCUS       DAMKMI010000080        18214 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMKMI010000080 DAMKMI010000000
VERSION     DAMKMI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.081"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            137..1540
                     /locus_tag="PC429_002388"
     CDS             137..1540
                     /locus_tag="PC429_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3847889.1"
                     /translation="MELDVIKKLIEQTNSKHSKFVKKADEAEKYYKNENDIIRDRSPN
                     NVGKVNTANNPLRNADNRIPFNWFGFLVNQKISYLFTYPPTFDVGDDGINSKITDILS
                     DRYPKEAKTLGKNASICSKAWLHVWEDDNNDFQYTNIDPRQIRAVYSSDLNRKLLAVL
                     REYKKTDDKGKEYVIYEYWTDECCYTYQNKDGNSNINGLEILNKFIEKNLDNKLETQT
                     NVYRHNFGEVPFIEFLNNDLEVRDLDNVKHLIDVYDKVYSGFVNDIEDIQEVIFVLTN
                     YGGADLTEFLKGLKEYKTIDLQSSGADDKSGLSTITINIPIEARDSLLKTTEKQIYVQ
                     GQGVDPKPENFANTSGVALKFLYTLLELKAGLMETEFRLGFAKLVRMICRHLGYSPKR
                     VLQTWTRNMIQNDLELAEICSKSVGIISEKTNLKNHPLVDNAEEEEKQIKKEKEDSNQ
                     EYNNVIPNNQDGVIDET"
     gene            1530..2891
                     /locus_tag="PC429_002389"
     CDS             1530..2891
                     /locus_tag="PC429_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF3847890.1"
                     /translation="MKHKDYWKKRFEQLEEAQNNKSIKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAESEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRHIYTSGYYHTAFNVQQGVNVGWS
                     LMNLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFAKKFNVSKSQAKNLIMTESAFFASASRKDCFSDLDVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPFFSDEEGYRVARDENGKTYYVPSGMKYK
                     EWYEKTFITDKKDDILSNKSWLKADFSTEKKFKKHVEKHLNEYGNITAEEYLSTARNL
                     LSEQLSNDVEGFLSKDKFLFKYRKSSNDFAVGRADGKISTLFKPKEGYNYWLEQIKEY
                     KEV"
     gene            2894..3055
                     /locus_tag="PC429_002390"
     CDS             2894..3055
                     /locus_tag="PC429_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847891.1"
                     /translation="MNCPVCGKDVDVFDICDNCNWQNSGPKETNYKGPNKMSLKEAKE
                     AYEKGKKVI"
     gene            3127..3321
                     /locus_tag="PC429_002391"
     CDS             3127..3321
                     /locus_tag="PC429_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847892.1"
                     /translation="MCILTQVIVVVCVVQIVINCIANVNVGILCNKLKKENKANIDKV
                     SDEILKRVMEELNKSLDESL"
     gene            3366..3938
                     /locus_tag="PC429_002392"
     CDS             3366..3938
                     /locus_tag="PC429_002392"
                     /inference="COORDINATES: protein motif:HMM:NF018515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF3847893.1"
                     /translation="MKKGELIVLGLSEEDAKKVEAESLKELENYINKIEYEKVKEELK
                     ASKEAIEGFKDGMTKEQIEELKKGYETKLTAKDEEYQNKLKEKEQKEFDMALENELIK
                     LNVHSTKAAKAELDLEKIKYENGTFTGLKEQTDTWLTQKSFLIKTGETKINYSPDNGN
                     KNTLSRAENIAKEKNEEGSKNPYADAWSIK"
     gene            3954..4334
                     /locus_tag="PC429_002393"
     CDS             3954..4334
                     /locus_tag="PC429_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847894.1"
                     /translation="MYFKEVNFDNTPEFLASQHYINFSKTALDTDVVADENGKKYVLA
                     GSLLGESGKVVKITRGGSSGSYTYTLSEDPVGIVFSTVDVTYGPQPVASMVEGYVIIE
                     RLQGEYVKEAIDTIKTKLPNIKFM"
     gene            4346..5365
                     /locus_tag="PC429_002394"
     CDS             4346..5365
                     /locus_tag="PC429_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDF3847895.1"
                     /translation="MARVEELLSVQELINYTKTRKLKETMGDLLFPTQKIEGLEIKMI
                     KGASNLPVSASVHAFDTEAEIASREGANLSIAELALVKRKIKLDEKDIIVLEQPRNSQ
                     EETQMINQIFNDVDNLVSSVNTRIEAMRMEVLSTGELNINENGVKASLKYGTPTNHKE
                     TKTWSSGTPDILGDIYNMTDKIVVDTGFTPTRSLTSKTILNIILRDEKLRKAIFGVNS
                     DKLLTLNELNTFLASQSLPPIFTYDERYRVQGKDGKYTTKRFLDENKFILMPDGKMGD
                     TFFGLTAEELELRKNPAIDISSVGNIIVEQYSTADPVAKWIKAVATALPSFPYADQVF
                     MGTIN"
     gene            5376..5720
                     /locus_tag="PC429_002395"
     CDS             5376..5720
                     /locus_tag="PC429_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HDF3847896.1"
                     /translation="MEVERLKKLLGFSREDDSKDTILEFILEDVEEMVKNYCNVPTIP
                     EQLNSTILKMAIDMYKNESLGSEDIALGSISSISEGDTSVSYRSSASEFKESLLKDYK
                     SQLNRYRKIRWK"
     gene            5720..6082
                     /locus_tag="PC429_002396"
     CDS             5720..6082
                     /locus_tag="PC429_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847897.1"
                     /translation="MMDKTRKAIEMLYRDKCTIVEYQPVKDPVTKRTNNKEVVVLENQ
                     PCKLSYKNITSTEQGEVAKLTQTIKLFISPNISVKAGSKLIITNQNNITKEYIRSGES
                     AIYPNHQEIVLELLEDKA"
     gene            6082..6507
                     /locus_tag="PC429_002397"
     CDS             6082..6507
                     /locus_tag="PC429_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF3847898.1"
                     /translation="MGRGGSVDFKELKKLQRNLQRLEESQLDKFCRDCAKELAARLIG
                     KAIKRTPVDTGVLRQGWNGVAYARSLPVIKAGDTYIIEIINPIKYASFIEYGHRTRNH
                     KGWVKGRYMMTISVQELRGQVDVIIEKKLINLLKKVFDA"
     gene            6500..6937
                     /locus_tag="PC429_002398"
     CDS             6500..6937
                     /locus_tag="PC429_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847899.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFICPLNP
                     SKTPYPNNRELKKNSFDVHYFPRSKDKNFEINEIAEMLLEELEYIEIDGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNDTEKMNDVELFGGLKRGDNFE"
     gene            6930..7106
                     /locus_tag="PC429_002399"
     CDS             6930..7106
                     /locus_tag="PC429_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847900.1"
                     /translation="MNKTLSKGTDYKFTKEQIVNSKKYINRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            7107..8417
                     /locus_tag="PC429_002400"
     CDS             7107..8417
                     /locus_tag="PC429_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF3847901.1"
                     /translation="MALGGGTFVTQNKVLPGAYINFISAKRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKFFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSIENKIKDKDLVESSLIYWVTGAIAGC
                     DINKSNTNKTYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSIRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNI
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            8437..8913
                     /locus_tag="PC429_002401"
     CDS             8437..8913
                     /locus_tag="PC429_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF3847902.1"
                     /translation="MSKNITMNVRDAISGSKAECFVTIKGKRYNFMQAINLEAKMEKN
                     KSEMPILGSITKGNKSTGSKFSGSATFYYNTSIFRELLYEYKETGEDIYFDMQITNED
                     PTSSVGRQTIILEDCNLDSGIIAKFDADGEYLDEDVDFTFENWRIVEKFNIANGME"
     gene            8965..9222
                     /locus_tag="PC429_002402"
     CDS             8965..9222
                     /locus_tag="PC429_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847903.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            9224..9664
                     /locus_tag="PC429_002403"
     CDS             9224..9664
                     /locus_tag="PC429_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3847904.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKPIEWELKAIDSD
                     RDRQLRKNSAIRVPVIGKKGKATGQYTSETDFNTYTLKLCVETIVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            9925..10692
                     /locus_tag="PC429_002404"
     CDS             9925..10692
                     /locus_tag="PC429_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4428 domain-containing protein"
                     /protein_id="HDF3847905.1"
                     /translation="MGLFGKKKADICCICNDEIGVLNIEDGWICNSCFKEYCDTLSMT
                     KAPKILRKLDIEKTISSTKKNNELQKIFNPTNNIENYIEFDEDNKKWLVPKRSVNDKK
                     APTIHSYENIAEFELLEDGESVVSGGLGRAVVGGALFGATGAIVGGIIGKKTTRKVVN
                     SFKIKITINNIDNPVEYIELINKKTKTNSSVYEKAYKDAHKILSTLSAITQSIKVTDN
                     INSSSVADEILKYKNLLDIEAITQEEFDIKKKELLNL"
     gene            10761..13133
                     /locus_tag="PC429_002405"
     CDS             10761..13133
                     /locus_tag="PC429_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF3847906.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTTSINTTINSLDRLQQRLHNPIN
                     AGSIQASQQSLNNIESILTRIEQKIKGNTNEQENFNNKIRQGSEAGSLLVSKLKSLAG
                     IYIGIRGIESITKAADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QVAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLAAKIADAMGKTKDQLKELSSSGAITADVIRNALFKASNEIEKKFA
                     SMPITFSQALTMMKNDAYMIFGQTLGKISGALQSVRFSEIVVSVRNVMIAISSNIYDT
                     LNIIKNILNSEFFSNLVQGFTFCAVIITKSLGSIVNTALNIVNIFAQNWSIIKPIVFG
                     VASVFMLFKGVLLATKIATISNVIANIAHAASSSLSALMTNIQAAALMRSNGATLSAT
                     IATWGLNAALLACPITWVVLGILAFVVVVFVAVAAVNKFAGTSLTALGVIVGAVFAAV
                     AFIQNIMIWLFNRCVDVNEGITNGWNQCVFLMKQAIAKGVIFIIEKMASLNDSVNNAG
                     NALGKAFVTGANIAIRGVNKLIDLLNKIPGINIGKVGEATFTPVKADNSHIKQQINSL
                     NRWVGDAPEKVKLERMGYKDIGAEFEKGNALGTKWQKSLEKKLKDTFDINKMLEDAKD
                     KLGLKDLWDKENPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKYLRDIAEQ
                     ETINRFTGVNIKIDMNNTNNISKDADVDGIVNVLTEKLNDAMVVSAEGIV"
     gene            13149..13850
                     /locus_tag="PC429_002406"
     CDS             13149..13850
                     /locus_tag="PC429_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF3847907.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKTVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSREMGGKVLFN
                     TNMKVSLEEYNIVEDAENASDTKVAIKLKQYRDYSTKKLVLATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNAKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKDKK
                     VPKYTIYKGQVLKLG"
     gene            13843..15801
                     /locus_tag="PC429_002407"
     CDS             13843..15801
                     /locus_tag="PC429_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3847908.1"
                     /translation="MDDELVLANDRDVRLVIAHWEDFYEPVVLDNITWEIERRGSPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMILTALDDTLRE
                     KKEMYVLYDDFGRLTLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     ALGDIRVRAGCLVPVFLSLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSTTSKEDNDMINKLNKVFKNKLSNTGSIFVKYSNAYK
                     VNAALMAAISIHETGNGSSPLCKNKNNFFGMKGMSFGSVDEGIKKGISNLSRNYIHIG
                     RKTLERIRDKYAPLYDSPLNKDWVPGVGKFYKQITGSTYSSNNAGTGVGSNEEAEKNL
                     KDTTYQVQNNNNANTSTNNSSKADKLISVAKSKLGCKYARGADGPNTFDCSGFTQWCY
                     KQIGIKIPRTVATQSKAGSAVDLKDRSKWKAGDLLCRVGGGSSNHVMMYIGNGQMIHS
                     PQTGDVVKIQSVDSYRKGKAYTHVRRFI"
     gene            15815..16075
                     /locus_tag="PC429_002408"
     CDS             15815..16075
                     /locus_tag="PC429_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF3847909.1"
                     /translation="MSQELLQIIKKTAIDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKVVLIRMQGGQQYLVLDRM"
     gene            16080..16499
                     /locus_tag="PC429_002409"
     CDS             16080..16499
                     /locus_tag="PC429_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF3847910.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFQNVKGKVHCKFTVYSKYGNIKAEKVVSV"
     gene            16500..17549
                     /locus_tag="PC429_002410"
     CDS             16500..17549
                     /locus_tag="PC429_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF3847911.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGQLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHRVTVEGVTSTTINISAEITYKSGYTWEN
                     IKTIAEEAVDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINSIASNL
                     NVDKDSIVLRGEVVG"
     gene            17542..18159
                     /locus_tag="PC429_002411"
     CDS             17542..18159
                     /locus_tag="PC429_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF3847912.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHDKLKDLSN
                     DQFLEDLTPSGVKRWEKIMSINPKSNETLEDRRFRIFSKYISKLPYSERFLRNWLDNV
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
BASE COUNT         7374 a         2010 c         3518 g         5312 t
ORIGIN      
        1 gaaagaactg gattatatct tgatgaacca gttgagttta tggatgatgc aatggcagct
       61 cttaggtatt ctatagataa taagcttaaa aataatgggg ttagaatact tactccaaat
      121 ggaagaaggt gataaattgg aactagatgt aataaaaaag ttaattgaac aaactaatag
      181 taaacatagt aaatttgtta aaaaagctga tgaagctgaa aaatactata aaaatgaaaa
      241 tgacattata agagatagaa gccctaataa tgttggcaaa gtaaatacag ccaataatcc
      301 actaagaaat gcagataata gaataccatt taattggttt ggatttttag tgaatcaaaa
      361 aatatcatat ctatttactt atcctcctac atttgatgtt ggagatgatg gtataaattc
      421 aaaaataact gatattttaa gcgatagata cccaaaagaa gctaaaacac ttggcaaaaa
      481 tgctagtata tgctctaaag catggttaca tgtttgggag gatgataaca acgactttca
      541 atatactaat atagaccctc gccaaataag agcagtatat tcatcagatt taaacagaaa
      601 gcttcttgca gtacttagag aatataaaaa gactgatgat aaaggaaaag aatatgtaat
      661 ttatgagtat tggacagatg aatgttgtta tacttatcaa aataaagatg gaaatagtaa
      721 tatcaatgga ttggagatac ttaataaatt tatagagaaa aatttagata ataaacttga
      781 aactcaaact aatgtatata gacacaattt tggggaagtt ccatttatcg aatttctaaa
      841 caatgattta gaggtaagag atttagataa tgttaaacac cttattgatg tatatgacaa
      901 ggtttatagc ggttttgtga atgatattga agatatacaa gaagttattt ttgttcttac
      961 aaactatgga ggtgcagact taacagagtt cttaaaagga cttaaagaat ataaaactat
     1021 tgatttacaa agtagtggtg cagatgataa gagtgggtta agtacaatta caataaatat
     1081 tccaattgaa gctcgagatt cacttcttaa aacaactgaa aagcaaatat atgtacaagg
     1141 tcaaggagtt gaccctaaac ccgaaaattt tgccaataca agtggtgtag cactcaaatt
     1201 cttgtacact ttgttagaat taaaagcagg acttatggaa acagagttta gattagggtt
     1261 tgctaaacta gtaagaatga tatgcagaca tttaggatat tcgcctaaaa gagttttaca
     1321 aacttggact aggaatatga ttcaaaatga tttagaacta gctgagatat gctctaagag
     1381 tgttggaata atatcagaaa agactaattt aaaaaatcat ccgcttgtag ataatgcaga
     1441 ggaagaagaa aaacagatta aaaaagaaaa ggaagatagc aatcaagaat acaataatgt
     1501 aattcctaac aatcaagatg gtgttataga tgaaacataa agattattgg aagaagagat
     1561 ttgaacaatt agaagaagca caaaataata agagcataaa atattatctt gaattagaaa
     1621 aacaatataa actagctatg aatagtatag aaaaagatat attagcatgg tacaacagat
     1681 ttgctgaaag tgaaggaata tctttattag aagctaagaa actactaaat acaagagaac
     1741 tagaagagtt taaatggagt gtagaagaat atattaaata tggtaaagaa aatgctataa
     1801 atcaaaagtg gatgaaagag ttagaaaatg ctagtgcaag agttcatata acaaggcttg
     1861 aagctttaaa gttacaaata cagcaacaag tggaagtgct ttatggaaat gaacttgatg
     1921 gtattgataa attaatgaga catatttata caagtggata ttatcataca gcttttaatg
     1981 ttcaacaagg agtaaacgtt ggttggagtt taatgaatct tgatactaat agaataaata
     2041 aagttatctc taaaccatgg actagtgatg gattaaattt tagtgaaagg atttggggca
     2101 aacatagacc tgctttaatt aatgagttac acactaaatt gacacaatca attattagag
     2161 gtgagaatcc aaaaaatcta gtcaatgatt ttgctaagaa gtttaatgta tctaaatcac
     2221 aagctaagaa tttgataatg actgaatcag ctttctttgc atcagcaagt agaaaagatt
     2281 gttttagtga tttagatgta gagaaatatg agattattgc tacattagat ttaagaactt
     2341 caaatatatg cagagagtta gatggaaaaa tatttgatat gaaagattat caagttggaa
     2401 taacagctcc accatttcat tgtcgttgta ggacaacaac agctcctttt tttagtgatg
     2461 aagaaggcta tagagtagca agagatgaaa atgggaaaac atattatgtg ccatctggta
     2521 tgaaatataa agaatggtat gaaaagacat ttataactga taaaaaagat gatatactaa
     2581 gtaataagag ttggttaaaa gcagatttct caactgaaaa gaaatttaaa aagcatgtag
     2641 aaaaacactt aaatgaatat ggaaatataa ctgctgaaga atatttatct actgcaagaa
     2701 atttgttatc agagcaattg agtaatgatg tagagggatt tttaagtaaa gataagtttt
     2761 tatttaagta tagaaagagt agtaatgatt ttgctgttgg aagagctgat ggtaaaatat
     2821 caaccttatt taaaccaaaa gaaggttata attactggct agaacaaata aaagaatata
     2881 aggaggttta gacatgaatt gtccagtatg tggtaaagat gtagatgtat ttgatatttg
     2941 tgataattgt aattggcaaa acagtggacc aaaagaaact aattacaaag gaccaaataa
     3001 aatgtcatta aaagaagcta aagaggctta tgaaaaaggt aaaaaagtaa tataaaagca
     3061 cttgctaaat aataaattag taggtgcttt tattatgtaa aaatttaaaa aaggtaggtg
     3121 attttaatgt gtatattaac tcaagttata gttgtagttt gtgtagtaca aatcgttatt
     3181 aattgtattg ctaatgttaa tgtaggtatt ctttgtaata aattaaaaaa agaaaataaa
     3241 gctaatatag ataaagtttc tgatgaaatt ttaaagagag taatggaaga attaaataaa
     3301 tcattagatg aaagtcttta agagactttt ttattttgta agaaatgaaa ggagaattaa
     3361 ataaaatgaa aaaaggtgaa ttaattgtat tagggcttag tgaagaagat gcaaagaaag
     3421 tagaagctga atcattaaaa gaattagaaa attatattaa taaaattgag tatgaaaagg
     3481 taaaagaaga attaaaagca tcaaaagaag caattgaagg ttttaaggat ggaatgacaa
     3541 aagagcagat tgaagagctt aaaaaaggct atgagactaa attaactgca aaagatgaag
     3601 aatatcaaaa taaattaaag gaaaaagaac aaaaagagtt tgatatggcg ttagaaaatg
     3661 aacttattaa acttaatgtt catagcacta aagcagcaaa agcagaactt gatttagaaa
     3721 aaataaaata tgaaaatggt acttttacag gactaaagga acagactgat acttggttaa
     3781 ctcaaaaatc tttcttaata aaaacaggag agactaagat aaattacagc cctgataatg
     3841 gcaataaaaa tacattaagt agagctgaaa atattgctaa agaaaagaat gaggaaggtt
     3901 ctaaaaatcc atatgctgac gcatggagta taaaataaaa aggaggataa agtatgtatt
     3961 ttaaagaggt aaattttgat aacacacccg agtttttagc ttctcaacac tatattaact
     4021 tttcaaaaac agcattagat acagatgtag tggctgatga aaatggaaag aaatatgtgt
     4081 tagcaggtag tttattaggt gagagtggca aagtggtaaa gataactaga ggaggttctt
     4141 cgggtagtta tacatacaca ttatcagaag accctgtagg aatagttttt tcaactgtag
     4201 atgttactta tggaccacaa ccagttgcat caatggtgga aggctatgta ataattgaaa
     4261 gattgcaagg tgagtatgta aaagaagcta tagacactat aaagacgaaa ttaccaaata
     4321 ttaaatttat gtaggaggat gaaatatggc aagagtagaa gaattattgt cagttcaaga
     4381 gctgataaac tatacaaaga ctagaaaatt aaaagaaaca atgggagatt tattatttcc
     4441 aactcaaaag atagaaggac ttgaaataaa gatgataaaa ggtgcatcta atcttccagt
     4501 atcagcaagt gttcatgctt ttgatacaga agcagagatt gcatcaagag aaggtgctaa
     4561 tttaagtatt gctgaacttg cacttgtgaa aagaaaaatt aaactagatg aaaaagatat
     4621 aattgtactt gaacaaccaa gaaattctca agaagaaact caaatgataa atcaaatatt
     4681 taatgatgtt gataaccttg tatcaagtgt aaatactaga atagaagcaa tgagaatgga
     4741 agttttatca actggagaat tgaatataaa tgaaaatgga gttaaagctt ctttaaaata
     4801 tggaactcca acaaatcata aagaaacaaa aacttggtct agtggaacac cagatatatt
     4861 aggagatatt tataatatga ccgataaaat agttgttgat actggattta ctccaacaag
     4921 gtcattaact tctaaaacta ttttaaatat aatcttaaga gatgaaaaac taagaaaagc
     4981 tatatttggt gtaaatagtg ataaattact tactttaaat gaattaaata catttttagc
     5041 ttctcaatct cttcctccta tttttactta tgatgaaaga tacagagtac aaggcaagga
     5101 tggtaagtac acaacaaaga gattcttaga tgaaaataag tttattctta tgcctgacgg
     5161 caagatggga gatactttct ttgggctaac agcagaggaa ttagaactta gaaaaaaccc
     5221 agcaatagat attagttcag ttggaaatat aattgtagaa caatactcta ctgctgaccc
     5281 agttgctaag tggataaaag cagttgcaac agcattacct agttttcctt atgctgacca
     5341 agtgtttatg ggtacaataa attagaggtg ttgatatgga agttgaaaga ctaaaaaagc
     5401 ttttagggtt tagtagagaa gatgattcaa aagatacaat actagagttt atactagaag
     5461 atgtagaaga aatggtcaaa aactattgta atgtacctac tataccagaa caattaaata
     5521 gtactatttt aaaaatggct atagatatgt ataaaaatga gagtctagga agcgaagata
     5581 ttgcactagg ctctatttct tctataagcg agggcgatac atcagtttcc tatagaagtt
     5641 cagctagtga atttaaagaa tctttactta aagattataa gtcacaatta aatagataca
     5701 gaaaaattag gtggaaataa taatggataa gactagaaaa gcaatagaaa tgctatatag
     5761 agataaatgt actatagttg agtatcagcc agtcaaagac cctgtaacaa aacgaactaa
     5821 caataaagaa gtagtcgtat tagaaaatca accttgtaag ttatcatata agaatattac
     5881 ttcaacagaa caaggggaag ttgctaaact tacacaaact attaaactct ttatatctcc
     5941 aaatataagc gttaaagcag gttcaaaact tattataact aatcaaaata atattacaaa
     6001 agaatatata agaagtggag aatctgctat atacccaaat catcaagaaa ttgtattaga
     6061 attactagag gataaagcgt aatgggtaga ggtggaagtg ttgattttaa agaactaaag
     6121 aagctacaaa gaaatctaca aaggttggaa gaaagtcaac tcgataagtt ttgcagagat
     6181 tgcgcaaaag agttagcagc aagattaatt ggcaaagcta taaaacgtac accagttgat
     6241 acaggagtgt tgcgacaagg ttggaatggt gtagcttatg caaggtcact tccagtcatt
     6301 aaagctggag atacttacat tatagaaatt attaacccca tcaaatatgc cagttttatt
     6361 gaatatggtc atagaactag aaatcataaa ggttgggtta aaggtagata tatgatgact
     6421 atttcagtgc aagaattaag aggtcaagtg gatgtaataa tagaaaagaa attaataaat
     6481 ttattaaaga aggtatttga tgcttaataa tataattgat ggaatatcag taaagttaga
     6541 taaatcattt ggagaaaaat atacaattta tagtgaggat gtagagcaag gtataaatga
     6601 gccttgtttt tttatttgcc ctctaaatcc aagtaagaca ccatacccaa ataacagaga
     6661 gttaaagaaa aattcttttg atgtacatta ttttcctcgt tccaaagata agaattttga
     6721 aataaatgag atagctgaga tgttactgga agaattagag tacattgaaa ttgatggaga
     6781 cttagtcaga ggtacaaata tgaactttga aattatagat aatgttcttc atttcttcgt
     6841 tgattataac tactttacta taaaaagtaa tgatacagaa aaaatgaatg atgttgaatt
     6901 atttggtggt ttgaagagag gtgataattt tgaataagac attaagcaaa ggaactgatt
     6961 acaagtttac taaggagcag atagttaatt ctaagaagta tataaataga aaagatttat
     7021 taaatgcaat tttaaaagaa aatgaattat attccttctc agaggtagag gatagaataa
     7081 ataaatttat gaaaggagtg agttagatgg ctttaggtgg aggaacattt gtaacacaaa
     7141 ataaggtcct acctggtgca tatataaatt ttatctcagc taagagggca accagttcat
     7201 tatcggatag aggtattgtt gcaatgcctt tagagttaga ttggggtata gatgaagaag
     7261 tattccaagt aaccagtgat gattttgaga agtattcaac aaagttcttt ggatatgatt
     7321 atacacatga aaagttaaaa ggtttgagag atttattcaa aaatataagg ttgggatatt
     7381 tctataaatt gaataaaggt gttaaagcta gttgtactat agccacagca aaatatagtg
     7441 gtatcagagg aaatgactta aaagtaacag ttacaacaaa tatagatgat aatgctaagt
     7501 ttgatgttgt aacactttta gataataaga aggtagatac tcaaatagca aaggttatta
     7561 cagacttaca agacaatgac tatatcactt ggaagaagga tgcaacacta gaagcaagtg
     7621 cagggctggt atttactggt ggaactaatg gcgaagctgt gacaggagca gagtaccaag
     7681 ctttcttgga taaaatagaa agctatagct ttaatgcttt aggatgtttg gctacaacaa
     7741 cagagattaa aagtttattt gtagaattta caaagagaat gagagataag gtaggagcta
     7801 agtttcaaac agtactatat aagaaaagtg atgcagatta tgaaggtgta gtgtctatag
     7861 aaaataagat taaagataaa gatttagttg aatctagctt gatttattgg gtaactggag
     7921 ctatagcagg atgcgatata aataaatcta atactaataa aacgtatgat ggtgagtttg
     7981 atgttgatgt taattatact caaatacaac ttgaagaagc tttaaaaact ggtaaattta
     8041 tatttcataa agttggtgat gaagttcatg tgttagagga tataaatact tttgtaagtt
     8101 ttacagacga taagaatgac gatttttcat ctaatcaaag tattagagta cttgaccaga
     8161 ttgctaatga tatagcgact ttatttaata caaagtactt gggtgaagta ccaaatgata
     8221 aatctggtcg tatctcgttt tggaatgatg tagttaagca tcatgaacaa ctacaaaata
     8281 taagagcaat agaagatttc aaagctgatg atgtttctgt agaacctgga agcgacaaga
     8341 agactgttgt agtaagtgat gctgttaagg ttattagtgc tatgagtaag ctttatatga
     8401 ctgtttcagt tagttaagag aggagtgtga taatagatgt ctaaaaatat tactatgaat
     8461 gttagagatg caataagtgg ttctaaagct gaatgttttg taacaataaa aggtaaaaga
     8521 tataatttta tgcaagctat taatttagaa gcaaaaatgg aaaagaataa aagtgagatg
     8581 ccaattctag gtagtattac aaaaggaaat aaaagtacag gaagtaaatt ttcagggagt
     8641 gcaacatttt attataatac ctctattttt agagagttat tatatgaata taaagaaact
     8701 ggtgaggata tttactttga tatgcaaatt actaacgaag accctacatc aagtgttgga
     8761 agacaaacta taatacttga agattgcaac ctagatagtg gaataattgc taagtttgat
     8821 gctgatggcg agtatttgga tgaggatgta gattttactt ttgaaaattg gagaatagtt
     8881 gagaagttca atatagcgaa tggtatggaa taagatacac atttatagtt tatagatgtg
     8941 tatttttatt tataagatta ggagatgatt aaaattaagg ataaatatga gataaaagat
     9001 tcaatttctt ttgattatag caataaaaga cctttggaag aacgtgtcag cgagatgtat
     9061 aaaaaggcag gaaaatatct tatagatatt tcagataagt tagcaacaga tacaattgat
     9121 ggttcgtcat taaagccaat aatcataaaa tttgaaataa atgaagccgg tgttgcaaca
     9181 atagaaaaac aaacaaaata tttagttatg gaggtagaat aatatgggag atttaaacgc
     9241 ttttttaagt caaaatgcaa taaaagtaga gaatagaaag tatgtggcaa gtgaaaggtt
     9301 tataggtgaa gatgggaaac caatagagtg ggaacttaaa gcaatagatt cagatagaga
     9361 tagacagtta agaaaaaatt ctgctatcag agttcctgtt ataggaaaaa aaggaaaagc
     9421 aacaggacaa tacactagtg aaacagattt taatacttat actttgaaat tatgtgtgga
     9481 aactatagta tttccggatt tacatgatgc agaattacaa aatagttatg gtgtaatggg
     9541 ggcagaggag ttattaacaa caatgttgac tcctggcgaa tatacagacc tttcaagtga
     9601 ggtaggagag gtaaatggtt ttgataggac ttttgaggat aaagtagaag aagcaaaaaa
     9661 ctaattgaag gaggcgatta tgatgctagt gtagctcatt attgccttca taaattcaaa
     9721 tggaagccgc atgaatatac agatttacca gactacgaga gggcttttgt tgctgcttct
     9781 atagatatta aagtagaaga agaaataaaa gaagaaaaaa agaatgtcaa agaagctaga
     9841 agaagtcgaa gaaggtaaat aataatgtaa aaattttacg catatagtat aattatccta
     9901 taaaatatta tagggggtaa tattatggga ttatttggaa agaaaaaagc tgacatatgt
     9961 tgtatttgta atgatgagat aggcgtacta aatattgaag atggttggat atgcaattct
    10021 tgttttaaag aatactgtga tacgctttct atgactaaag cgcctaaaat tttaagaaaa
    10081 ttagatattg aaaaaactat atcatcaact aaaaaaaata atgaactcca aaaaatattt
    10141 aatccaacta ataacataga aaactatata gaatttgacg aagataataa aaaatggctt
    10201 gtgcctaaaa gaagtgtaaa cgataaaaaa gctcctacca ttcattctta tgaaaatatt
    10261 gcagaatttg aacttttaga agatggagag tcagtagtta gtggaggatt gggaagagct
    10321 gtagttggag gagctttgtt tggtgctact ggagctatag ttggtggaat aataggtaaa
    10381 aaaacaacaa gaaaggttgt taattcattt aaaattaaaa taacaattaa caatattgat
    10441 aatcctgttg agtatattga attgattaat aaaaagacca aaaccaattc cagtgtttat
    10501 gagaaagcat acaaagatgc acataaaatt ttatcaacat tatctgctat tacacaaagt
    10561 attaaagtta cagataatat aaattctagt tctgtagctg atgaaatatt aaaatataaa
    10621 aatcttttgg atattgaagc tatcacacaa gaagaatttg atattaagaa aaaggaacta
    10681 ttgaatttat aatacataag cacttactta aaaataggta agtgctttta ttattccaaa
    10741 aaagaaagga gagtgaaaat atggctacaa tacaaacttc aataaagatt ttcgacggaa
    10801 tgacacctgc atttcgcaat atgactacat ccattaacac aacaattaat agtctagata
    10861 gacttcaaca aagattgcac aaccctataa atgctggtag catacaggca tcccaacaaa
    10921 gtctaaacaa tattgaaagt attcttacta ggatagaaca aaaaattaaa gggaatacaa
    10981 atgaacaaga aaactttaat aataaaataa gacaggggag tgaagcaggt tctctattag
    11041 tatctaaact aaaaagtttg gctggtatat atattggaat aagaggtata gaaagtatta
    11101 caaaagcagc agatacaatt gcaagtacaa aagcacgttt aaatttaatg aatgatggct
    11161 tacagacaac agaccagctt aataaaatga tttatttgtc agcccaaagt gcaagagcta
    11221 gttatgcaga tacagcagca caggttgcta aacttggaat acttgcagga gatgcttttg
    11281 gaagttcagc agaggtgata aagtttacag aacttatgaa taaagctttt gtaattggag
    11341 gaacatcagc aaatgaagct agtgcggcta tgtatcagct tactcaagct atgggtgcag
    11401 ggaaacttca aggtgatgaa tttcgctcta taatggaaaa tgcaccattg ctagctgcta
    11461 aaatagctga tgcaatggga aaaactaaag accaattaaa ggaattgtca agtagtggag
    11521 caataacagc agatgttata agaaatgcac tgtttaaagc ttctaatgag atagaaaaga
    11581 aatttgcaag tatgccaatc actttttctc aagctctcac aatgatgaaa aatgacgctt
    11641 atatgatatt tggccagaca ttaggaaaga taagtggagc attgcaaagt gttagattta
    11701 gtgagattgt tgtatctgtg cggaatgtta tgattgcaat atcttcaaat atttatgata
    11761 cattaaatat tataaaaaac attttaaata gtgaattttt ttcgaacttg gtacaaggat
    11821 ttacattttg tgcagtaata ataactaaaa gcttaggttc tattgtaaac actgcattaa
    11881 atatagttaa tatattcgca caaaattgga gcataataaa acctattgta tttggtgtgg
    11941 cttctgtttt tatgttattt aagggagttt tgttagcaac taaaatagct acaataagta
    12001 atgtaattgc aaatattgct catgcagctt catcttcact atcagcactc atgacaaata
    12061 tacaagcagc agccttaatg cgttcaaatg gagctacttt gtcggcgaca atagctactt
    12121 ggggtttaaa tgcagcttta ttagcttgtc cgataacgtg ggtagtatta ggtattctag
    12181 catttgtagt agtagttttt gttgcagtag cagcagtaaa caaattcgca ggtacaagtt
    12241 taactgcctt aggagtaatc gtaggtgctg tatttgcagc agtagcattt atacaaaaca
    12301 taatgatatg gctgtttaat agatgtgtag atgtaaatga aggcattaca aatggttgga
    12361 atcagtgtgt ttttcttatg aaacaagcaa ttgcaaaggg tgtaatcttt ataatagaaa
    12421 aaatggcatc attgaatgac tctgtgaata atgcaggtaa tgcacttggt aaggcttttg
    12481 ttactggagc taatatagca atacgaggtg taaataaatt aatagatttg ctaaataaaa
    12541 tccctgggat taacattggg aaagtaggag aggcaacatt tacaccagtt aaagcagata
    12601 atagccacat caagcaacaa atcaacagtc taaatagatg ggtaggagat gcaccagaga
    12661 aagttaaatt ggagcgaatg ggatacaaag atattggagc agaatttgag aaaggaaatg
    12721 ctctaggaac taagtggcaa aaatctctag aaaagaaatt aaaagatact tttgacatta
    12781 ataagatgct agaagatgca aaagacaagc taggcttaaa agatttgtgg gataaagaaa
    12841 atcctttaaa taatcttggt ggatttggtg gagatttagg aaaaaatgta aaggacacag
    12901 caggaaacac tgcaaaaatg gctaaaacta tggataaaag tcaagaagac cttaaatact
    12961 taagagatat tgcagagcaa gaaacaataa accgatttac aggagtcaac attaaaattg
    13021 atatgaataa cacaaacaac ataagtaaag atgcagatgt tgatggaata gtaaatgtct
    13081 taacagaaaa actgaatgat gctatggttg tatcagcaga aggaatagtt tagaaaggag
    13141 ggatataaat ggcttatgat ttttacctag atggagtaca attaccaatc cctccaccca
    13201 agttagaaat taaagtgaca aacaaaaata agacagttga tttaataaat actggagaag
    13261 taaatatatt aaaaaaagaa gggctatctg agataagttt tgaagcagag tttacacata
    13321 ataaactacc attttatcgt ggaactttta gggatgttca attcttttta agtaaactgg
    13381 aattactaaa gacagattgt aagccatttc aatttattgt ttcaagagaa atgggtggaa
    13441 aagtattatt caacactaat atgaaagtgt cattagaaga atataatata gtagaagatg
    13501 cagagaatgc ctcagataca aaagttgcaa taaagttaaa gcaatataga gattattcaa
    13561 ctaaaaagtt agtacttgca acacctgaaa agacaaatta tggtaggact ccccctccag
    13621 tcatgaaacc aaaagaattt agaccagatt catctaataa gccaaatgct aagacatata
    13681 cagtcaaggc aggagattct ctttggtcaa tctgtcagaa acaacttggt aatggttcat
    13741 tatataagaa agtatatgag ttaaataaaa caatgatgga taaagctaac aaggacaaaa
    13801 aagtacctaa atacacaatt tacaaagggc aggtgttaaa acttggatga tgaattagtt
    13861 ctagcaaatg atagggatgt aaggttagtt attgctcatt gggaagattt ctatgagcca
    13921 gttgtccttg ataacataac atgggaaata gaaagaagag gttcaccttc taagttagag
    13981 tttacaatag tcatggatga tatattagag ttttgcgaag gtaactctgt aaggctgtat
    14041 tataaaggaa taggcatatt ttatggatat atatttcaaa agaaaagaga taaagaaaat
    14101 cacattaaaa ttgttgctta cgaccagttg agatatttta agaataaaga tacttatgta
    14161 tatagtaata aaacagcaag tgaacttgta aagatgttgg ctaaagattt taatttaaaa
    14221 tacaatgtca tagaagatac aaaatataaa atatcgagag ttgaagaaaa taaaacactc
    14281 tttgatatga ttttaactgc acttgatgat actctaagag agaaaaagga aatgtatgta
    14341 ctctatgatg attttggaag attaacatta aagaatgttg cttctatgaa acttgatacg
    14401 gttatgaaca atgatgtaat tgaggacttt gactacaatt cttcgattga tagtgatact
    14461 tacacaaaga ttaaacttgt gagagataat gaagaaacag ggaaaagaga tgtatatatt
    14521 gcacaagact ctacacatat gaggagttgg ggaatacttc aaatgtttga tacagttgat
    14581 aaaaatatga gtgaagcaga gataaaacaa aagtgtgata tacttttaaa actatataat
    14641 aagaaaacta agtcattaag tttaaaaaat gcacttggag atattagagt aagagcaggt
    14701 tgtttagtac ctgttttttt gtcgttagga gatattaaac ttcaaaatta tatgctagtt
    14761 gaaaaagtta agcacacatt tgaaaataac agtcacttta tggatttaac tcttgttgat
    14821 ggagacgaat ttgcttctta ttcttcaagt tcatatagta gtggaaacac taataataaa
    14881 gatgaaaaga aaaatggtcc tgcacaaagt actacaagta aagaagacaa tgatatgata
    14941 aataaattaa ataaagtatt taaaaataag ttatcaaata caggaagtat atttgttaaa
    15001 tattctaatg cttacaaagt caatgcagct ttaatggctg ctatttctat acatgaaact
    15061 ggtaatggaa gttccccact ttgtaaaaat aaaaataatt tctttggaat gaaaggaatg
    15121 tcttttggct ctgtagatga agggataaaa aaaggtatta gcaatttatc aagaaactat
    15181 attcatattg gaagaaaaac attagagcgt attagagaca aatatgcacc tctttatgat
    15241 agccctctta ataaagattg ggtaccaggt gtaggaaagt tttataaaca aataacagga
    15301 agtacctata gttctaataa tgcaggtaca ggagttggaa gtaatgaaga agcagaaaag
    15361 aatttaaaag atacaactta tcaagttcaa aataataaca atgctaatac atcaacaaat
    15421 aatagttcta aagcagataa actaattagt gtagcaaaaa gtaaattagg ttgtaaatat
    15481 gctcgaggag cagacggtcc taatactttt gattgttctg gattcactca atggtgttat
    15541 aaacaaatag gtataaaaat tcctaggact gttgcaacgc aaagtaaggc aggcagtgca
    15601 gtagatttaa aagatagaag caagtggaaa gcaggagact tattatgtag agttggtgga
    15661 ggtagtagta atcacgtaat gatgtatatt ggaaatggtc aaatgattca ttcaccacag
    15721 actggtgatg tagtaaaaat acaatcagtt gattcatata gaaaaggaaa agcatataca
    15781 catgtgagaa gatttatata agtgaggtga taaagtgtca caagaattat tgcaaataat
    15841 taagaagact gcaatagatg cagtagaaac aagcaatcca atgagagttg tatttggaac
    15901 aatagaaagt attagtcctc taagagttaa gatagaacaa aaactatcta ttggtgaaat
    15961 ttttctaata caaacagata catttaaaag atatacagat aaaaaaatag gagataaagt
    16021 ggttttaatt cgtatgcaag gaggacaaca atatttagta ttggatagga tgtgatgaag
    16081 tgttaccaag cgataattta gattatgata ttgaagatgt atcaataatt aattttgatg
    16141 taagacaaga gccaagtaag acatttaaac ttcatataga aaaatctaag attgatggta
    16201 tttgtgatga tgtagaagca ttaaaacaga ccatcttttt gattttaaac acagagagat
    16261 accaacatct aatatatagt tggaattatg gagtcgagtt gaacgacctt attggagagc
    16321 ctatatcctt tgtaatcccc gaacttgaaa gacgaatcaa agaagcacta attcaagatg
    16381 atagggttga aaatgtagat aattttgagt ttcaaaatgt aaagggtaaa gtacattgta
    16441 agttcacagt ttacagtaaa tatggaaata taaaagcaga gaaggtggtg agtgtataat
    16501 tgtttgagtt aatgacattt gaaaatataa ttaaaagaat gttagatagt gtaccagata
    16561 cttttgataa aagggaaggc tctataatat ataatgctct tgctcctgtt gctatagaac
    16621 ttacagaaac atacattgca atggatgaat tactagacca aacatttgta gatactgcta
    16681 gttattacta tttagagaag agatgtaaag agagaggtat tacaccttta cctgcaacca
    16741 atacaattgc aaaaggagtt tttaacatag atattccact tgattctagg tttaatctag
    16801 gagaatacaa ttatattgca attgagagaa tatctgaaaa aacatataaa atgaaatgtg
    16861 aaactgctgg acctattttt gagttagggc aattaatacc tattgaatat atagatggtc
    16921 ttgaaactgc tgaactaact gaaatcttga taaatggaga ggatgaagag tcagaggaca
    16981 gtctaaggca acgatattat gatagtttaa attcacaatc atttggtgga aatatgcaaa
    17041 attataaaga tgaagttaac aaaatacaag atgttggagg agttaaggtt tatcctgtgt
    17101 gggacggtgg aggaactgtt aagttagtaa taattaattc taatttcaaa gtaccatcag
    17161 aggatttagt taatttagtg caagaagaaa ttgacccaat tggacatcaa ggacaaggct
    17221 taggattagc accaattgga caccgagtca cagttgaagg agttacaagt acaactataa
    17281 atatatcagc agaaataaca tataagagtg gatatacatg ggagaatata aaaacaattg
    17341 cagaagaagc agttgatgac tatttaaatg aacttaatat gagttgggaa gatgaagaaa
    17401 acttaatagt tcgtatatct caaattgaaa ctagattact tagtattgat ggagtattag
    17461 acattgcaaa tacaatgata aatagcattg catctaatct taatgttgat aaagattcta
    17521 tagtattaag aggtgaagta gttggataaa gagattaatc taataaatta cttgccacaa
    17581 attctacaag ataaagaaga atatataaaa gtatttaatg tagaaaacaa agaaataaaa
    17641 acactacatg acaaattaaa ggacctatca aatgaccagt ttttagagga tttaactcca
    17701 agtggtgtga agagatggga aaaaataatg tctataaatc ccaagtcaaa tgagacttta
    17761 gaggatagaa ggtttaggat ttttagtaaa tatataagta aactacctta ctcagagaga
    17821 tttttaagga actggctaga taatgtagtt ggagaaggca attatgaatt aactattaat
    17881 aatgctactt ataacataca ccttgagagt gatgctagaa atcaagattg gtttgaggaa
    17941 gttcattctt ttgtaagtaa tattaagcca tgtaacatga cactagatta cactagagtg
    18001 cttataagca aagacaatta tatgaatttt ggtataacaa ccttaatggg tcaagaaata
    18061 actatatacc cttggagtcc accagatata gaaacttatg gagaaattga tgtattaact
    18121 ggcaatggag ttggatacca agagataaca atattttagg aggtgatata ttggctatag
    18181 ataaaagtta ttacactata attacagatg tagg
//
LOCUS       DAMKMI010000081        18055 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMKMI010000081 DAMKMI010000000
VERSION     DAMKMI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.082"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            105..1535
                     /locus_tag="PC429_002412"
     CDS             105..1535
                     /locus_tag="PC429_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF3847913.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            1903..2481
                     /locus_tag="PC429_002413"
     CDS             1903..2481
                     /locus_tag="PC429_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF3847914.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            2511..2657
                     /locus_tag="PC429_002414"
     CDS             2511..2657
                     /locus_tag="PC429_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF3847915.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            2962..4341
                     /gene="scfB"
                     /locus_tag="PC429_002415"
     CDS             2962..4341
                     /gene="scfB"
                     /locus_tag="PC429_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF3847916.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            4370..4867
                     /locus_tag="PC429_002416"
     CDS             4370..4867
                     /locus_tag="PC429_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF3847917.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            5165..6829
                     /locus_tag="PC429_002417"
     CDS             5165..6829
                     /locus_tag="PC429_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF3847918.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            6842..9292
                     /gene="recJ"
                     /locus_tag="PC429_002418"
     CDS             6842..9292
                     /gene="recJ"
                     /locus_tag="PC429_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF3847919.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDDSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            9305..9817
                     /locus_tag="PC429_002419"
     CDS             9305..9817
                     /locus_tag="PC429_002419"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF3847920.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            9891..12098
                     /locus_tag="PC429_002420"
     CDS             9891..12098
                     /locus_tag="PC429_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF3847921.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            12123..12572
                     /locus_tag="PC429_002421"
     CDS             12123..12572
                     /locus_tag="PC429_002421"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF3847922.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            12587..13201
                     /locus_tag="PC429_002422"
     CDS             12587..13201
                     /locus_tag="PC429_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3847923.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            13292..14848
                     /locus_tag="PC429_002423"
     CDS             13292..14848
                     /locus_tag="PC429_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF3847924.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            14838..16100
                     /locus_tag="PC429_002424"
     CDS             14838..16100
                     /locus_tag="PC429_002424"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF3847925.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            16174..17961
                     /gene="aspS"
                     /locus_tag="PC429_002425"
     CDS             16174..17961
                     /gene="aspS"
                     /locus_tag="PC429_002425"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF3847926.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMLTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         7349 a         1796 c         3372 g         5538 t
ORIGIN      
        1 gacaatgaat agacttttaa ataaacttaa tataaaatta attaaataag tgcttaaaaa
       61 cgatttactt taatgataga atcatattaa aggaggataa ttttatggat ataaagaata
      121 aaattaaatc tatattttta ccagaaaaaa atgactattt agatgatgaa gaatttgagt
      181 atgatataga aaataatgaa gaagatttaa aggaagataa caaagtacat ttaaatgatt
      241 taagcagagt aaaatatgat tacagagagt taaaagacat agaaaaccaa acagaagaga
      301 tgtgtctgct agcagtaaag caggatggta ctataattga gtttgtaaag gacaagactt
      361 ataaagtatg tatggaagca gtaaaacaga catataaatc tcttaagtat ataacaaatc
      421 aaaatgagga tatttgtata gaagcagtaa aacagaacta cagagctttg tactacataa
      481 ataataagac agaaaatgta ctaattgagg ctataaaaaa tgcatcaact tatgatgtca
      541 tggaagtatt taaatttgtg gaagagcaaa cagaagatgt atgtctagca tttatagaaa
      601 gggcatctaa aaatgatgtt gcagagatat taaaaggaat aaaagagcaa actccagcca
      661 tatgtttaga agctgtaaaa aaagatggaa aatctttagc ttatgtaaag gaacaaagta
      721 atagtatatg tttagaagct gtaaaagaaa actacagtgc actatcttgt gtaaaagagc
      781 aaactgaaga aatatgtata gaagcagtaa aacagaatga ctttgcattg tattatgtaa
      841 atgaacagac agaaaaaata tgtatggaag cagtaaaaag aagttatatg gctctacagt
      901 atgtaaataa gcaaacagaa gagatttgtt tggaagcagt aagaattgat ggaagagcat
      961 tacagtacgt aaaagagcaa acagaagaaa tttgcttgga gtcagttaga caaaatggaa
     1021 aagtactgca atatgtaaaa aaacagactg aaaatatatg tatagaagca gtaagaggtt
     1081 catttgaaga acttgaaata aaagaaatat taagttatgt aaagatacca acagaaagaa
     1141 tatttgtaga agcagtaaag caaaatggaa aaattcttaa atatgtagaa aatcagacag
     1201 aattaatatg tttagaggca gtaagagaaa actataatgc tttagcttat gtaaaggaac
     1261 aaacagaaaa gatttgttta gaagcagtta atcaaagtta tgaagcgctt aaatatgtga
     1321 aagaacaaac agaagaagtt tgtctaaaag cagtaaaaca agattataga atgttgaaat
     1381 atgtaaataa tcaaacagaa aaaatatgtt tagaagcagt aaagcaaaat tatagagctt
     1441 tagaatttgt tgataatcaa acagaaaaag tttgtcttga agcagtaaaa cagaatagaa
     1501 aagcactaca gtatgtaaaa caaaaacaaa gttaaaaggc taaaatatat agaaaacata
     1561 tgcaaataaa taaaatttga tattttgtaa taaataaaat gggtatctca aaaggaactt
     1621 tttagtttgt gaggtactct ttttatatga aatgaaatat atttttagcc ttttaacaat
     1681 ggggaaaagg tttatttcta ttttgacata ggctttttat gcatataaag cttgataaaa
     1741 ttgttttatt ataattgttt tgttatataa ataataagaa gaaaattata taggtctttt
     1801 tttgaccagt tttacatttt taacaactag ttttgtaaag tatattgaat tttctcaaaa
     1861 aagaatgtac tctatatatg taaaattttt ttggaggtaa aaatgtttaa aagatatgct
     1921 gaaaaaatga catctgtttt aatttgtaat aatatgatag ataataatga atctaaagtc
     1981 tattcttatg gatttgaaat attaatagct ttcatagtta atataaccac catgctgttt
     2041 ataggttttt tatttggtaa atttacatat gtattatttt ttttaatgtg ttattgtcca
     2101 ataagacaat tttcaggagg gtatcatgca gacaattatt ttagatgctt attaaccttt
     2161 atttttataa ttttatctac aatcttaatt atagaaaata taaatataga cttatttaaa
     2221 aatattatta tgattattgc aagtgttagt tgggttggaa tttgtgttct gtgcccaata
     2281 gaacatagga gtaaccctat aagtgatagg gaaaaattgg tttataagaa aactgctata
     2341 tttatatcta cagtagtgtt attgataaca attttaagtc tttctttgag tatatttgta
     2401 gattatttca catatagcgc atttgccatg ttttggattt ttgtgatgtt agtattaggt
     2461 aagttaaaag ctaaagtata agtaaaatat gtatgaggga gagttaagtt atgaaaaagt
     2521 ttattgtaag atttatgaag tttgctagct cattggcact tagcactgct attttgtcag
     2581 ctaatagtac ctgtccttgg attattcatc agcctaaagt acccaaagaa atcagcaatc
     2641 taaagaagac aaattaaata caagcactta tttatacaaa attatcagga aaaaagaaat
     2701 tattatattt taataagatt aaaacaatat aaaatcagat acaaaattta ttttatttat
     2761 tttataggag cctaatacct atttggtaat atattagtgc gccaatttag ggggatatag
     2821 tgtatttaat gaaatgactt acggagagta agtcattttt gtattgtatg tattttatgt
     2881 aaacttatga tataatgaag ggtaaagttt aattaaaata ataaaaaact ttagtataga
     2941 tagtaattta ggaggtaggg tatgagtatg atacataaat tttctatgaa tggatataat
     3001 atcgttttag atgtgaatgg tggagctgtt catgtgttag atgatgttgc atacgatttg
     3061 ttagattttt ataaagaaaa aagtaaagaa gaaatattag agatattaaa atcaaaatat
     3121 caagaagaaa aaataaatga agcatatgaa gaaatattaa atttggaaaa agagggtctt
     3181 ttatatacag aagatacata tcaatatcat ccaagctttg tacatagaga gcctgtagta
     3241 aaagctttat gtttaaatgt agctcatgat tgtaacttaa aatgtaaata ctgttttgca
     3301 gctcaaggtg attttggagg agaaaaagaa cttatgagtt ttgaagttgg taaggcagca
     3361 attgactatt tgattgctaa ttcaggaagt agaaagaacc tagagataga tttctttggt
     3421 ggagagcctt tgatgaactt tgaggttgta aaacaactgg ttgactatgg aagaagtgta
     3481 gaaaaagatt ataataaaaa tataagattt acaataacta caaatggtgt actgctaaat
     3541 gatgagatta tagattatat aaatgaaaat atgcataatg tagttttaag tttagatgga
     3601 agaaaagaag ttaatgataa tatgagacca actttaaatg ataaaggaag ttatgatatt
     3661 acattaccac gatttaagaa acttgtggaa aaaagagcaa aagataagta ttattatata
     3721 agaggtactt ttacaagaga taatttagat ttttcaaaag atgttatgca ttttgcagat
     3781 ttaggattta aattaacctc tgtagagcca gttgttggag atgagagtaa tccatatgca
     3841 cttagagaag aagatttacc taagatattt gaagagtacg aaaaatttgc tgtagagtat
     3901 gcagatagac aattacaagg agatggattt aaatttttcc attttatgat agacctaaat
     3961 caaggtccat gtgtaataaa gagaataact ggatgtggag ctggaaatga gtatctttct
     4021 gtaactccaa atggagatat atatccatgt catcaatttg ttggaaatga agaatttaaa
     4081 atggcaaata tatttgatga agaaatagta cttccagaaa atttaaaaaa tatgtttaga
     4141 gaagctcatg tatatactaa agaagagtgt aaacaatgtt ggaataagtt ttactgttca
     4201 ggtggatgtc atgcaaatgc gataaacttt aataatgaca tatcaaaacc atatgaatta
     4261 ggatgtgaaa tgcaaagaaa acgtacagag tgctctataa tgatacaagc taagttaatg
     4321 ttagaaggag caaccaacta aatttatatt ttaaaggagt tgtttatata tgattagaga
     4381 ttatttagaa gataaacctt taattgacga aagtgtattt gtggcaaaat cagcagatgt
     4441 aattggaaat gttaaaatag gcaaagattc aagtatatgg tacaatgcag tagttagggg
     4501 tgatgaagga ccaataacta ttggagaaaa cactaatata caagattgtt ctatagtgca
     4561 tggagataca gaaactataa ttggtaacaa tgtaacagtt gggcataggt ctatagttca
     4621 tggttgtaaa attagtgata atgtgttaat tggtatgggt tcaataatat tggataatgc
     4681 tgaaatagga gaatatactc tgataggagc aggtacacta attacatcta ataagaagtt
     4741 tccaccaggt gttttgataa tgggttctcc tggtaaggtt gttagagagc ttacagaaga
     4801 agataaaaaa tatattgatg aatcttatga atggtattta gaggcagcac aaaatcaaaa
     4861 atattagttt aaagagtggt tgtttcaaaa catatttaaa tctgttttga ggcaaccact
     4921 aatttatttt attagatttt gttgatattt tagttttcaa aaagtatttg attaaaccag
     4981 atataaaatg tcaaaaataa aaattaactg agttaaataa ttttaataaa attggtagta
     5041 tatgtgataa aattaaatat taaagaggat ataggatatt agaatatcaa ttggtgaaat
     5101 aatgtcatat acgataaatt aaggtgtaag tttttgggta tacctatctt aggagtgaaa
     5161 agatttgaga ataagttata gaaatttaaa gcgatataga gaaattggat atgttttaat
     5221 aaagtatggt tttagtttta ttgtagaaag actaaatata gaaggaattg cctataaaat
     5281 accactattt gacccaccag aagaaataaa aaatatgaca actggtgaga gaatgaaaag
     5341 agttttagaa gaactaggac ctacatatat aaaaatagga cagatattga gtacaagaaa
     5401 agacttgtta gaccaggata taattgatga aatatcaaaa ctcagagatg atgtagagaa
     5461 atttgattca aatatagcta ttgatatatt taaagaagaa gtgggtttaa gtatagaaga
     5521 aatattctta gaatttaaag aagaaccaat agcagcagct tcaattggtc aagtttatga
     5581 aggtgtactt aaaacgggag aagaagtaat cgtaaaaatt caaagaccaa atatagagaa
     5641 aataataaaa tctgatttag agattcttag aactattgca aatactttaa aagatttaaa
     5701 aaaggatttt aatttagatt tagtacagat gattgaagaa tttcaaactc aattaatgag
     5761 ggaacttgat tatacatttg aagctataaa tgcaactaaa ttctctagga tatttaaaaa
     5821 tagtgatgaa gtatatatac ctaaagtata ttcagaatat aatactaaga aaattttagt
     5881 aatggagaaa gttaatggta caaagttaag tgatgtagaa aaaataagga gactaggtta
     5941 taacacaaaa actattgttg aaataggagt taggtcattt tttacacagg tattatctca
     6001 tggatttttt catgcagacc cacatccagg taatatattc gtagttgcta aaaataagat
     6061 agcatatata gattttggaa tgattggaat tattgataat aaaactttaa accaattaaa
     6121 tgaaattgca ttagcaggtg tagaaaagaa tgttgacaag ataatatatc tattgataga
     6181 aatggatgca ttaaatggag aggctgatat taagggactt agacaggatt tactttatct
     6241 aatacattat tactatgata tttctataga aaaaataaat gtaacagata tattaaatga
     6301 actatttagg ttttttagac agtataaaat agtcatgcct gcacaatttg taactcttgc
     6361 aaagacagtt ataacattag aaggaacaag tagaacttta aacacagatt tttcttttgg
     6421 ttctatggga aaagagttta tgaaacatca ttataagagt aaatttaacc ctaaaaatgt
     6481 tgttttaagt tcaagacaaa atgtagaaga aatattactg gacataaaaa ctattccaaa
     6541 gcaattaaaa gcaattttaa aaaatataga aagaaataat ataaaaatgc aaattgaaga
     6601 tgttaaaatg acgaggttag aaaattgtat aatagagcta acatctcaaa tatctttgag
     6661 tttagtgttg gcatctatta tagtaggttc ttctcttata attgcatctc caaatataga
     6721 aaataatata tggataaaat ttactgcaat agcgggtttc tttatatcct ttataatagg
     6781 cctttgctta gttataagaa gtattaggtc aaaatacaaa aaagattagg gggaattaaa
     6841 tttgaaaaaa tggatattga agtataaagg taagatagag gaaagtgaat ttagcaaaaa
     6901 acttaatatt tccccggaaa tctgccaaat tttaaaaaat agagggatat gtactgaaaa
     6961 agattcagaa atatttatga atccatcttt agattattta agagaccctt ttttaatgaa
     7021 agatatgcaa aaagctgttg atagaataaa gttagcagtt gagaagaacg aaaaaatttg
     7081 gatttacgga gattatgatg ttgatggtgt atcatctaca tctatcttgt gcttatattt
     7141 taagagtata ggttacaatg ttgattacta tattcctaat agattagaag aagggtatgg
     7201 tataaatgaa gatgctatta agcttataag ctctagggga tgtgacttga ttataagtgt
     7261 tgactgtgga attacatcag tgtctgaggt caatacagcg aatgatttgg gcatagatgt
     7321 tataattaca gaccatcatg aatgtcaaag tgaaattcca tcagcctttg cagtaataaa
     7381 tccaaaacaa gaagattgta attatccatt tgattcacta tgtggatgtg gtgtagcatt
     7441 taaaatgatt caagccttga ctccaaaaga agaatttaaa actagtatgt ataattattt
     7501 ggaaatagta acactagcaa ctatttgtga tattgttcca cttatagatg aaaatagaat
     7561 aattgtaaaa aatggtttaa aatcaatgaa agaaggcaaa aatattggat taagggagct
     7621 tataaaagtt tgtggtgtag aaagtgataa gataggttct tctcatatag gttttgcaat
     7681 aggtcctagg ataaatgctt caggtcgatt gggatattct tacttaggag tagagctatt
     7741 tactactcaa tcacaagaag aggcagtaga aatagctagt atattggaag aaaaaaataa
     7801 tgaaagacag atgatagaag ctaagatgta tcatgaagct gaagaaatgc taaagtctaa
     7861 tagcagatac aatgatgaca aggtgttggt tttagctaaa gagggttggc aacatggaat
     7921 aataggaatt gtagcatcaa agttaactga aaaatactac aaaccaacaa ttcttctagg
     7981 tatagaaaat ggggaggcta ctggttcagc gaggtcaata aaaggattta atatatttga
     8041 agctctcata aagtgcaaag acttgatgac taagtttggt ggtcatgaac aagcagcagg
     8101 gttatccttg gattcagaca atgtagaaat tttagcaaat gaaataaata aatttgcaga
     8161 ttataattta actgaagatg atatgataga gaatgtaaat gttgagtttg aattacaaga
     8221 aaatgtaata aatttaaatc ttgtagaaga attacataag ttagaaccct ttggattaaa
     8281 taatcctaat cctagattta tagttagaaa ttatatttta aaagatttga aagttatagg
     8341 taagaatcaa caacatctga aattaagcat agaaaaagag aaaagctatg aatgtatagg
     8401 atttaatatg agtcatttaa aatcaatgta taaagtagga gacaaggtag atgtactatt
     8461 ccaattagat gaaaataact atatgggaaa taggaaagta caatttttac ttaaagatat
     8521 acgattggct cgacctaaaa gtgcaagtaa tgacaaactt tcattgaaat taatgtcaaa
     8581 aataatacca aaagatactc aaagcctgta taatatatct gtgtctgatt ttgaattatt
     8641 tgatggaaat acagatataa atatatttga ttattttgaa aaagatacac taataatatc
     8701 taattctata aatggatttt atagagcaat gtcagatata tcacttatag atttggattt
     8761 taacataaat tacaatatta ttgaagatga tagcaaaaat actgataaat tagagcttat
     8821 attttctcct aatattgata aaatagactt aaaaagatat aataatatta ttctttatga
     8881 ttatttatac aataaaggcg aatattcgta tatatatgag aataaaagag aagaaagtga
     8941 aattataaaa tattataata aaacagacct attatattta aagaatgtag ttagtaatat
     9001 agttccatca agagatgaat ttataacaat ttataaacaa gcactcataa aaaaagaaat
     9061 tgatttggat atggtaaata taagggaaac ttttaatgta attccattaa agttttttac
     9121 gatattaaat gtatttagag aattaaattt acttgacttt aatttaaatt atgaaaaaaa
     9181 ttctgtactc ataagaatat tgccaaaacc acagaaaaaa ttagacttaa atgagagttt
     9241 aatactcaat aatctaaaaa atttagaaaa acaatacaat agcagttatt aggaggcgtt
     9301 tcccatggat ttaaaaaact ttataaggaa tattgatgat ttcccaaagc caggcataga
     9361 ttttaaagat gtgacaactc tatttaaaga tggagatgca tttaaatatg cagtggattc
     9421 tatagttgaa gaattgaaag ataaagatgt agatttagta ataggaccag aagcaagagg
     9481 atttttgatg ggtacacctg tagcatatgc tttaggagta ggatttgttc caattagaaa
     9541 acctggaaag ttaccaggtg aagttgagag ttatgagtat ggattagagt atggtacaga
     9601 tactttagag atacataagg atgcaattaa aaagggtcaa aaagtagcaa tagtagatga
     9661 tttattggct actggtggaa ctatggaagc cgctgccaag cttgtagaaa aattaggtgg
     9721 agaagtagtt tctatgcaat ttttgattga gctaaaattc ttaaatggga gagaaaagtt
     9781 gtctaactat gatgttaatt ctcttataaa atactagtac ttttataata gttggtataa
     9841 caccaactat tttcatatat attaaaattt tatacaaaat aggtggttat atgcatgata
     9901 aagaattaca agaaataatt gaaaaaataa aagtttatgc acctaatgca aattttgaaa
     9961 tggttgaaaa agcctataat cttgcaaaaa aagcgcatga aggacaatat agaaaatctg
    10021 gggaaccata ctttattcat ccattagcag ttgcaaatat tttaatagag atggaattag
    10081 atattgaaac tataacagca ggtttacttc atgatgttgt agaagataca gaatatacct
    10141 atgaagacat aaaaaaagaa tttggcaaag aagtagcgga tttggttgat ggagtaacta
    10201 aacttggtca aataaaatat cgttcgaaag aagaaactca atctgaaaat ttaagaaaaa
    10261 tgtttttggc aatggcgaag gatataaggg ttatacttat aaaacttgct gatagattgc
    10321 acaacatgag aacattaaaa tacatgcctc cagaaaaagc aaagtataaa gcaaaagaga
    10381 cacttgaaat ttatggtggt atagctcata gattaggaat ttccaagata aagtgggaac
    10441 tagaagacag agcattaaga tttatggacc cagaagggta ttatgattta gttagtagag
    10501 tatcaatgaa gagaagtcaa agagaggatt atattcaagg aattgttgaa cttcttaaag
    10561 ataagtttaa agatttaagt ataccatgtg atgtatatgg tagaccaaag catttttaca
    10621 gtatatatag aaagatgcaa aaaaaacata agacttttga agagattttt gatttaacag
    10681 cagtaaggat attggttgat aatgtaaagg attgctatgc agtacttggt atagttcata
    10741 cattatggag acctattcca ggaagattta aagattatat agcaatgcca aaacccaata
    10801 tgtatcaatc attacacacc actgttgtag gtcctgatgg agaaccttta gagatacaaa
    10861 taagaactca tgagatgcat aatatagctg aaaatggtat agcagcccat tggaaatata
    10921 aagaaggtat ctctagcaaa gagtcaaagg ttgaagaaaa acttcaatgg ttgagacaga
    10981 tgatggagtg ggaaaaagat ttaaaagacc cacaagagtt tatggatgca cttaaagaag
    11041 atgtatttaa tagtcaagtt tatgtattta cgccaaaagg agacgtaatt gaattaccag
    11101 cagaatcaac acctatagat tttgcctata gagttcatac aaatgtaggt aataaatgtg
    11161 taggagctaa aattgatgga agaatagttc ctattgatta taagcttcaa aatggtaata
    11221 ttgtcgaggt acttacatca tctaattcta atggaccaag tagggattgg cttaatatag
    11281 tgaagacccc aaatgcaaaa agtagaatta gacaattttt caagaaggaa agaagagaag
    11341 aaaatgttga ccgtggaaat acaatattag aaagagaatt taagaagcat gatataccac
    11401 aaaaagaccc tcttgttgaa aaatatatgg cattagttgc taagaaattt aatcagccaa
    11461 gtgttgagga cttaattgcc actgtaggtt atggtggtat aatgtcatct caagtggtat
    11521 ctaaggtaaa agacctatat ttaaaagagg ttaaaaaagc taaaaaggaa caacagatac
    11581 aagaagaaga tttaaataaa cacaatataa gtgaaacaga atacagtaaa aagagaaaga
    11641 aaaacgcatc acaaggaatt atagtaaaag ggcttgacaa tatcttagta agatttgcta
    11701 agtgttgtaa tcctcttcca ggagatgaaa ttgtaggata tattactaag ggtaggggag
    11761 tagccataca cagagctgat tgcccaaatg caaacaataa tggtgatttc ttccaaaata
    11821 gattggtgga ggtttcatgg agttcagcag gaaaaggtaa gtttgaagcg gaaattcaaa
    11881 taaaagctat tgacagaaga ggaattgtta atgaaataac tcatattgtt gcaatggata
    11941 aacttagttt aaacggaata aatgcaagaa aaggtaagga tagtattgta agtataaact
    12001 tacttgtaga ggttaatgac acagaggaat taaaaattct aatgaaaaaa ttaaaagcaa
    12061 ttcctggtgt agaaagtatt tatagaatga caaattagag ttaaaataat agggaggaaa
    12121 ctatgagagc agtagtgcaa agagtatctt catctaaagt tacagtagat gaaaacacta
    12181 taggtcaaat aaataagggg ttactagtat tgttaggagt gactcatgat gataaatcaa
    12241 gtgatgtaga ttatatgatt gataaaatat tgaatctgag aatttttgaa gatgaaaatg
    12301 acaagatgaa tttatctcta atggatatag gtggagagct tttagtagta tctcaattta
    12361 ctttatatgg agattgcaga aaaggaagaa gacctggatt tagtaatgca gctagaccag
    12421 aacttgcaaa taacctttat gaagaatttg taaaaaaagc aaaagataaa ggtgtgacag
    12481 ttggtacagg tcaatttgca gctcatatga tggtagagtt aactaatgat ggtccagtaa
    12541 caatactatt agactcaagt aaatcttttt aagggggaaa actttaatga ttataaaaaa
    12601 atttgtagat agttattttg gagtaaatac ttatgtatta ggtgatgaaa agactaaaaa
    12661 atgtgcagtt atagacccag gtggaagttt agtagagaca ttaagttttg taaaagaaaa
    12721 tgaattaaat attgaatata ttatccttac tcatgggcat ggagaccata taggatatgt
    12781 taaggatatt aaagaaaaaa ctggtgctaa agtagttgca catgttgatg aaaaagaatt
    12841 attaaatgac aaaaataaaa atctaagtta tacaatgaga tgtggagcgc aagagtttga
    12901 tgcagatatc tatgtaaatg ataaagacaa gcttgaactt ggtgaattaa aaatgacttt
    12961 cttgcatact ccaggacata ctcaaggatg tatgtgtata agagttgaag atgaaatgtt
    13021 cacaggagat actttgtttg ctggaagtat tggtagaact gatttatata gtggagactt
    13081 taaccaaatg gaaaaatcac ttaagaaatt atgtaaatat gaagatgctg ttagagtata
    13141 tcctggacat ggaccaacca gtactctagg ggttgaaaag aagacaaatc cttacatgta
    13201 attttatatt agtatttaaa aaagtataaa atatttaata actatattag tgagggggtt
    13261 cttctactgt taaatagaag gagttaagtt gatgttgtat gtttttttaa aaggacatga
    13321 ttataagtat gaagtagcag aacttataaa attgtttact agtgaattta agtttaaaga
    13381 ctctaatgct aacaataata tagagtcaaa gcatttaata aatagattga tttatgagaa
    13441 tggtgtatta ttctcaagta ctgaatacta tgaaaatgga aacttaaagt acgagtcaat
    13501 acagaatata gaaaatatga atcttgaatt tttagatgga tttgatagca gcaaaacgat
    13561 tgaagaattt cttaatagcc tagatagtga aagtaagagg aactttaaaa agctgtgtaa
    13621 agaaaatatc aaaaaaagca tgtttttagt gcttaaacaa gtatttaatt cttatgtacc
    13681 atggggaatt ttgactggta ttagaccagt caaaattgtc cacaatttaa tggataaaaa
    13741 gctagatgat aatagcataa gaaccattct aaaagataat tattttataa tagatgagaa
    13801 aatagattta gctcttgaaa tagctaaaag agagcgatta tttatgtatc ctatagataa
    13861 aaataaaatt tctctttatg taagtattcc attttgtcca actagatgtg tatattgttc
    13921 atttccttca aattcattaa aacaatttgg acatttaaaa agagaatatg tttataaatt
    13981 gattgaagaa attaaggggt ttgccaaagt tattaaggat actaagaaag aagttgaaac
    14041 tctatatatt ggtggtggaa ctccaactac acttgatgag gaagaattgg atttgcttat
    14101 aaattcattg ttcaatgaat tagatttaag taaaattaaa gaatttactg tagaagcagg
    14161 tagacctgac actattacag aacaaaaact aagagtgtta aaaaaacaca atgttagtag
    14221 aattagtata aatcctcaaa ctatgaatga tgataccttg gtaaagattg gaagagagca
    14281 tagagtatct gatttagtag attgctttaa tatggctcgt agaatgggat ttgataatat
    14341 aaatatggat ataattttag gtcttataga tgaagactta aatatggtaa agaacacatt
    14401 ggaggagata aaaaaacttt ctcctgaaag ccttacagtt catacattag ccataaagag
    14461 agcatctaac ttaaatataa atatggatag gtataaagaa catctaacac aatatgaaga
    14521 aatggtaaaa atgatagatt tatcaatgaa atatgcaaaa gatatgggat taaatccata
    14581 ttatatgtac agacaaaaac atatgttggg aaatttagaa aatataggtt atgctaaaga
    14641 aggatatgag tgcatttata atatacagat tatggaagaa aaacaaagta actatgcatt
    14701 aggtgcaggt gctatatcga aatttgtcta tgtagatgaa gatagaattg aaagagtaga
    14761 aaatgtaaaa aatgttgaac aatacattga aagagtagat gaaatgataa aaaggaagaa
    14821 agaagaggta tataaaaatg ttgactaaag ctcctagagg aactaaagac ataactccaa
    14881 aagaggctta caaatggcgt tatgtagaaa ataaatttag agaaatatgt gctttatatg
    14941 gatatgaaga aatggtaaca cctatatttg aacatacaga acttttcaag agaagtgttg
    15001 gagatactac agatatagtt caaaaagaaa tgtattcttt taaagataaa ggtgatagag
    15061 aaattaccct aaagccagaa ggtacagcag gtgtagtaag agcatttata gaaaataaat
    15121 tatatgcaga tactcaacca acaaaattat tttatgtgac accttgtttt agatatgaaa
    15181 gaccacaagc tggtagacaa agacaatttc accaatttgg aatagaagca cttggaagtg
    15241 atactccttc aatggatgct gaaataatcg cactagctgt tcaatttttt aatgaagttg
    15301 gattaaatga tttggtagtg agtataaatt cagttggctg tccagtttgt agaaaagagt
    15361 acaatgcttt attaaaagag tatcttgact caaaagctga tatattgtgt gatacttgca
    15421 atgagagaag agaaaaaaat ccaatgaggg ttatagactg taaaaatcct acatgtaaag
    15481 aaaatatcaa agatatacca tttattgcag accatttgtg tgatgattgt aaatctcact
    15541 ttgataaact tcaagaatac ttaaaggaaa tgaatataaa ctttgtaatt gataaaacaa
    15601 tagttagagg cttagattac tatagaaaaa ctgcttttga aattatatct aatgatatag
    15661 gagctcaaag tactgtatgt ggtggaggaa gatatgatgg tcttgttgaa caattaggag
    15721 gaccaaaagg tataagtgga attgggtttg gactaggaat tgaaagactt ttacttacac
    15781 ttgaaggtaa tggaattgaa atagaaaacc ctcaatctac ggatatattt atagtaacta
    15841 taggtgaaga agcaaataca agaagcttta aattgttaaa agacttgaga caaaatcata
    15901 taagtgcaga taaagaccat atagagagaa gtgttaaagc acaatttaaa tattcagata
    15961 aaataaattc aaaatttaca atagtaattg gagatgatga gcttaaaaat gacactgcga
    16021 cattaaaaaa tatgaaaaca tcagagcaaa ctactgtaaa attaagtaca ttagttgaag
    16081 aattaaagca aaagctgtaa aataagaaag tatgatgttt tgtgtattag ataaatatat
    16141 agcattaaaa ttaaataaaa tggaggtagt atcttggaaa ctttaaaggg tttaaagaga
    16201 actcactact gtggagagtt gagagaaaaa aacataaatg aagaagtcgt acttatggga
    16261 tgggtacaga aaaaaagaaa tttaggtggt cttgtatttg ttgatttaag agatacaagt
    16321 ggattatgtc aaatagtttt tgatacagat gtagataaag aagcttttga aaaagctgaa
    16381 aaattagggg ctgaatttgt aatagctgta aaaggaaaag tatgcgaaag acaatctaaa
    16441 aaccctaata tgctaactgg tgatatagaa atatttgcaa ctgaacttag acttttaaac
    16501 aagtcagaaa ctccgcctat ttatataaaa gatgatgatg atgtttctga agcactaaga
    16561 ttaaaatata gatacttaga tttaagaaaa ccatctatgc aaagaaattt aaaattaaga
    16621 cataaagtaa tgaatattac aagaaattac ttatcaaaca atagattttg cgaaatagaa
    16681 acaccatttt taatagctcc aacaccagaa ggagcaagag attatctagt accaagtaga
    16741 gttaatccag gaaaatttta cgcattacca caatcaccac aattatataa acaattattg
    16801 atggtaagtg gtatggatag atatttccaa atagttaaat gctttagaga tgaagactta
    16861 agagcagata gacaaccaga gtttacacaa atagactgcg aaatgtcttt tgttgagcaa
    16921 gaagatgtta tgagtatgat tgaaggtttg cttgaagcga tatttaaaga agttttagat
    16981 gtagaattgg cacttccact tcctaagatg acttatgcag aagctatgtc taaatatggt
    17041 tcagataaac cagatactag atttggatat gagttaacag atatatctga tgttgtttgt
    17101 aactgtggat ttaaagtgtt tgctgatgct acacaaccag gaaaaagtgt tagaggtata
    17161 aacgtaaaag gcaaagctga cgactttact agaaaacaaa tatcatcact agaagaacat
    17221 gctaagacat atagagcaaa aggtcttgct tggatgaaag taggacaaga aggagtaact
    17281 tctccaatag caaaattctt taatgaagaa gaaatgaacg ctatacttac aagaatgaat
    17341 gctgaagtag gagatttact tttatttgta gctgataaaa actcaatagt atttgatgct
    17401 ttaggacaag ttagacttga agtagcaaat aggcttaatc tattggataa aaatgtatat
    17461 aacttactat gggtaactga gttcccagtt tttgaagaag atgaagaaac aggcacattc
    17521 tcagcaatgc atcacccatt tacatcacct atggatgaag atttagacaa gttagaagaa
    17581 ggtgataagt catctttaag agcgaaagct tatgatattg tattgaatgg ttatgaaata
    17641 ggtggaggaa gtgttagaat atcaaattct gatgttcaat caagaatgtt taaagcactt
    17701 ggttttacag aggaaagagc taatgaaaag tttggttatt tattagaagc atttaaatat
    17761 ggtactcctc ctcatgctgg tctagctttt ggtcttgata gacttgttat gttacttgca
    17821 ggaactgata atataagaga agttattgca ttccctaaaa atcaaaatgc tgtttgccct
    17881 atgacaaatg ctcctacatt ggcggaagat gagcaattag aagaattaag tataaaagtt
    17941 gatataaaag ataatgaata atatgtagtt gcaagaaact aaaatgtaaa aagtctggat
    18001 ataaaatata tttatattca gacttttttt attagaatta ttttatattt tttct
//
LOCUS       DAMKMI010000082        17929 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMKMI010000082 DAMKMI010000000
VERSION     DAMKMI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17929
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.083"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            164..1537
                     /locus_tag="PC429_002426"
     CDS             164..1537
                     /locus_tag="PC429_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3847927.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYTLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
     gene            1793..2860
                     /locus_tag="PC429_002427"
     CDS             1793..2860
                     /locus_tag="PC429_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF3847928.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKINFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            2981..3289
                     /locus_tag="PC429_002428"
     CDS             2981..3289
                     /locus_tag="PC429_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF3847929.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            3282..4712
                     /locus_tag="PC429_002429"
     CDS             3282..4712
                     /locus_tag="PC429_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF3847930.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            4790..5164
                     /locus_tag="PC429_002430"
     CDS             4790..5164
                     /locus_tag="PC429_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF3847931.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            5158..7362
                     /locus_tag="PC429_002431"
     CDS             5158..7362
                     /locus_tag="PC429_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF3847932.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            7362..8771
                     /locus_tag="PC429_002432"
     CDS             7362..8771
                     /locus_tag="PC429_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF3847933.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIKYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            8756..9820
                     /locus_tag="PC429_002433"
     CDS             8756..9820
                     /locus_tag="PC429_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF3847934.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            9850..11271
                     /gene="nhaC"
                     /locus_tag="PC429_002434"
     CDS             9850..11271
                     /gene="nhaC"
                     /locus_tag="PC429_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF3847935.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            11700..12164
                     /locus_tag="PC429_002435"
     CDS             11700..12164
                     /locus_tag="PC429_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF3847936.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GPYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            12398..12760
                     /locus_tag="PC429_002436"
     CDS             12398..12760
                     /locus_tag="PC429_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3847937.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            13344..14180
                     /locus_tag="PC429_002437"
     CDS             13344..14180
                     /locus_tag="PC429_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847938.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            14264..16378
                     /locus_tag="PC429_002438"
     CDS             14264..16378
                     /locus_tag="PC429_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF3847939.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            16919..17872
                     /gene="blaCDD"
                     /locus_tag="PC429_002439"
     CDS             16919..17872
                     /gene="blaCDD"
                     /locus_tag="PC429_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF3847940.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
BASE COUNT         7144 a         1945 c         3325 g         5515 t
ORIGIN      
        1 aaattttaaa tatacttaaa aatatattaa tataatgtac ataaaaaata tcataaatat
       61 atgataagat aaatacaata gcaacatttt attaattttt tgggttttta tatatttagg
      121 tactggggtg atatagttga accaagagat attttttaaa ataatgaatg aaatattgga
      181 aaatattgat gaaggggtac attttgttaa taaagacggg aaaactataa tctacaataa
      241 ttctgtacaa aaaatggaaa aaatgaacag acacgatatt ttaaccaatt cattttttga
      301 tatagtcaac aaaatgaaaa taaacaggag tacactatta gaagttttgg aaaaaaggga
      361 agttatcaaa gacaatattc aaaaatatct ggataaaaat ggaaaagaga taacagcaat
      421 taatactaca ataccaattg atattaaagg cgagtttgta ggggcacttg aaatatcaaa
      481 gaatatgaca acgatagaaa atttactaaa tagaaattat aaaaaaaatt tagtcaataa
      541 agttagtatt agacaaaaaa agggttatga atttgatgat attttagggg aaagtaattt
      601 gattaaagaa gcaatagcta aatctaagaa agcttgcaag tctgatgctt cagtatttat
      661 atacggtgaa actggaacgg gtaaagagtt atttagccag tccattcact atggaagtaa
      721 taggagaaac tatccattta tagcacaaaa ttgtgcagct ttgccggaat cactattcga
      781 aggtattctt tttggaacta gcaagggagg atttacagga gcaatagata ggccaggctt
      841 atttgaacaa gctaacaaag ggacattgtt attggatgag ataaattcca tgcctattat
      901 gttacaggct aaactattaa gggttttaca agaaggttat gtaagaaggg ttggaggaac
      961 taaggatata cctatagatg taagaattat tgcaacgaca aatgaaagtc caaaggtaat
     1021 tttaaacgag cataaaatgc gaaaagattt atattatagg cttaacataa ttcatataaa
     1081 aattcctcct cttagagata gaaaagagga catacctcta ttgtgcaaaa aatttatttc
     1141 aaagtataat aaaaagttaa ataaaaaagt ggtaggaatt tcagatgaag ctattaaaat
     1201 tttaaaaaat tacgattggc aaggtaacat aagagagcta gaaaacataa tttattgtac
     1261 tatgagtatg atggaagatg aggtacaaat agaaacaagt atgattaatc taaacgacta
     1321 ttacactctt aaagagaata aagagagtaa caattattct ttaaacgatt tggaggacaa
     1381 aacacttagt aagattatca gtgagataga agaaaagtat atatatgaat cattagaaaa
     1441 ggcatcttac aatataacta aagctgcatc aattttaggt atgaataggc aaaatttaca
     1501 gtataaaata aaaaaatata atatcaaaat attataaata agcaagataa aaaaacaaaa
     1561 tcaaagtaaa ataaaaaagc aaaatagagg agcaaaatta ttataaaaga gcaaaatatt
     1621 ttgctctttt ttttatttct cataagaaat attatttttt atttatgaaa atacataata
     1681 aaataattat agtttcaacg ttccaataaa tatttacttt aattttaaag ttggcacgat
     1741 ttatgcttta actaattatt gagaatatat aaatttttag gagggtgtat atatgagaaa
     1801 agtaagagta ggaatatggg gatttggagc tatgggaata ggtatggcta atatgattct
     1861 aaagaaagaa ggtatagaaa tagtatctgt ttgtagtaga agtacttcag ggaaaagtat
     1921 gtatgatgtt ttaggaattg aaagaggaga aagaccagaa gttattatca ataaaaatta
     1981 tgaagaagtt tttagagaaa aaagtgtaga tgttgttttg ttagcaactg attcatttac
     2041 taaaaaggct tttgataaaa taaatttctt attaaataga aaaataaatg ttatatcaac
     2101 agcagaacaa atggcatatc cacaagctga tgatgcagat ttagctaaaa aaatggatga
     2161 agttgcaaag gaaaatggag taagtatact tggaacagga ataaatccag gatttgtatt
     2221 agatttacta gtattggcac tttcaggtac ttgtgaagaa gttactagta taaaagctaa
     2281 gagagtaaat gatttatctc catttggaaa gtctgttatg gtggaacaag gtgttggagt
     2341 aacaagggaa gaatttataa aaggtgtaga agataagact atagcaggtc atgtaggttt
     2401 tgtagaatca ataaatatga tagctgatgg attaggttgg aaattagata agatagaaca
     2461 aacaaaagag ccaataatga ctacagtaga tagaaaatct aagtacggag aggcattggc
     2521 tggaaatgtt gctggttgta gacaatgtgg atatggatat gtaaatggag aagttttaat
     2581 tgaaatggag catccacaac agataatacc agaagcagaa ggtattaaaa caggagacta
     2641 tgtatcaata aaaggtatac caaacattga tttacaaata aatcctgaaa tacctggagg
     2701 agtaggtact tatgcaatga tagtaaactc tattccatta ataataaatg ctagaccagg
     2761 tcttaaaact atgttggata taccagtacc tagagcaata atgggagata taagaaatca
     2821 gatagaagtt gaattggaag aagaatcaaa ggctaattag taagatagca actagctaat
     2881 tagtaagata acagttagct aattggtaaa ataatgttta atttaaaata acagtaaatt
     2941 aattagtaag gcaatagtca attaataagg ggatgaaaaa atggatgcta aaattaatga
     3001 ttgggttatt atacacaata tagtattgac tccagaagaa agagctccac aagttccaga
     3061 ggatactaag aaagtatcct tagaaatgtg ggttaaaggt tttatacaaa gtgatgcttc
     3121 tataggcgac ttagtagaag ttaaaacaat tacaggcaga cttgtaaaag gtaatttact
     3181 taaaataaat ccatattata ctcatgatta tggtaaatgt ataccagagt tactccaaat
     3241 aggaatccaa gctaaagaaa tattatttgg aggtgtatat aatgaataat tcaacatcaa
     3301 tgaaagatat gagttatcaa gctgttatgg gaagaaataa cgaaataatg aaaaatgcaa
     3361 tcggattgga ttattcttct tttgaacaag agggaatagg gtttgattat gagaaaatga
     3421 tgagtgaaac aggatataca ttacaagata ttgaagcaat tcaatcacag tatgctgttg
     3481 gaaatactcc tttaatagag cttaaaaatt tgactaaatt agctagaaaa tgtgctaaag
     3541 aaggtaaagg agctagaata tttgtaaaag atgaagcaat gaatgcatct ggtagtttta
     3601 aagcaagaag agcagctact gcagtatatc atgccaaaca aatgggatat aaaggtgtaa
     3661 tagcagctac tagtggtaac tatggtgcag ctgtagcatc acaagcggca atgcagggat
     3721 taaaatgtat aatagttcaa gaatgttatg attctaatgg tgtaggtcaa cctgaaatca
     3781 tagaaaaagc gcgaaaatgt gaggcatatg gagcagaagt tgttcagtta actgttggac
     3841 cagagttatt ttatacgttc ttagttttat tagaagaaac aggctatttt aacgcatcat
     3901 tatattcacc atttggaatt gcaggtgtag aaactttagg ttatgagtta gctatccaat
     3961 ttagagaaaa gtataaaaaa gaccctgata tagttgtttg tacaaatgca ggtggtggta
     4021 atcttactgg aactgcaaga ggattgataa aagctggttc tattaatact aaagtagtag
     4081 gagcaagtgt agatttaaaa ggattacaca tggctagtga taatcaattt aataaaaaat
     4141 catttacaac aggacataca ggttttggga taccatactg tacatggcct gataggtcag
     4201 atgtacctag gtctgcagct agaccactta gatatatgga tagatatgta ttagtaaaac
     4261 aaggagaagt tttttataca actgaattat tggcacagct agaaggtata gaaagaggtc
     4321 cagcaggaaa tacatcttta gcagcagcat tctctttagc tcaagagcta gatgaggata
     4381 aagctatagt ggtacaagaa acagaatata cgggtgcagg taaacatata tgtccacaac
     4441 ttacctttgc aagagaaaat ggaatagata taaaatttgg aaatccaaga gaagaaattg
     4501 caggagtaaa cttaatatta ccagaaagac cagaattatt gaaatgtgtt gatattgata
     4561 tgaataaaat aagaaagtca tttataaaaa attgtatatc taataatcac attgataata
     4621 ttgataagct aagtaatcga gatatagaat ttttaatgga agaagtaaaa agttcaagag
     4681 attttgttgt tgatgtatta aataatttat agtaattatt gttttttaat ttaatataaa
     4741 ataaaaattc agaaaattcg attaaatcaa tacgaatgga ggaatgttca tgaaaaaaag
     4801 agaggatgat tttgaagtta gaagaaaaca tcttcaagaa ctgagtgatg atgagttaaa
     4861 agaaaggttt tggagtttag ctacccagat agtagaacca atgatagaac ttggtaaaaa
     4921 gaatacaaca ccatcaatag aaaggtctgt cttgttgaga atgggatttt cttctcttga
     4981 agtaaaacct attttagaag gggttatgga aagaggtctt ataggtaaag gagcagggca
     5041 tgtagtttat aaattggcta aatctaaaaa tataacggtt agagaagcag gtttgttatt
     5101 agttaagggt gaatattggg atgaagttac atgtttattt agagaagggg tagaatcatg
     5161 ttaaaggaaa ataaaaaatt ggatatagac tatatattaa aagatttgga taaatataaa
     5221 ccaaaacgaa gaggttgggt ttggagagaa cacttggaaa atcttgaaat gggacctttt
     5281 aaatataaag actgtacaaa acctttaaag aagagtgtag ggctaccatc atctaagtat
     5341 ttcaataata tagaccctca accaagtcca gttattacta ctgaaatagc ttctggtaga
     5401 tttgaggatg atataagaag aatgagaatg gcagctcatc atggagctga ccatctaatg
     5461 gttatcagaa ctgctggaca atcacatttt gatgggctta tagaaggaac tccacaaggt
     5521 ataggtggag ttccaataac aagaaaacaa gtaagagctc aaaggaaggc tcttgatttt
     5581 atagaagaag aagttggtag acctattaat taccattcat atgtaagtgg tgttgctggt
     5641 cctgaagtag cagtaatgtt tgctgaggaa ggagtcaatg gagcacatca agacccacaa
     5701 tataatgttc tgtataggaa tataaacatg gtaaggtctt ttgtagatgc ttgtgaggca
     5761 aagaaaataa tggcttttgc caatatagct caaattgatg gagcacataa tgctaatgct
     5821 actgctagag aagcatggaa ggtaatgcct gaacttatgg ttcaacacgc tttaaactct
     5881 atattctcag aaaaaatagg tatagataaa agtaatatat gcttatcaac agttccacca
     5941 acagcacctc cagcaccatg tttaaaaata gacttacctt acgcggttgc acttagagag
     6001 ttatttagtg attataagat gagagctcaa atgaatacta aatatatgga atcatcaact
     6061 agagaagcaa cagttactca tgtattaaac ttattaacat cagtattaac tagagctgac
     6121 atacaatcaa ctataacacc tgatgaaggt agaaatgtac cttggcatat atacaatata
     6181 gaagcatgtg atacagcaaa gcaagcttta gttggtatgg atgggcttat ggatatgata
     6241 gaacttaaag atgtcggtga attaagagac aaagcaagag aacttaaaga aagagctgta
     6301 ttatatatgg aagaaatatc tgaagttggt ggatattttg aatctgtgga acaaggattt
     6361 tttgttgatt ctggaaatta tccagaaaga aatggtgatg gaatatctag aaaaataaaa
     6421 ggtggagttg gtgaaggtac tgtttatgaa agagaagaag attatttagc acctgtaact
     6481 gctcactttg gatacaataa tgtagctcaa tatgatgaaa atgctataga taatccatca
     6541 atgttaatag atggatgtac atttgaaaat ccagacaaaa taatatatat tgatgagctt
     6601 gacgattttg ataatgtaga aaatagacta aaagaatcat atgagtacag aaatggaact
     6661 aaaattaagc ctgaaatgga atggtgtgca gatggaattg taatgataac aatgatgctt
     6721 cctactgata aaagaacagc agagtttgca gctttggaat ttgtcaaaaa aatgaactta
     6781 caagaaattg aggtcataag tagagaagtt atgcatgaat ctgaagggac tagaatagaa
     6841 gtgaaaggaa gagttccgtt tgatatagat ttaaacaatt tagtaatacc agaagaacct
     6901 aaggtactaa ctgatgaaga aataagagca gatatagaag aaaaacctat gaaaatagtt
     6961 tcagctacgg ttggagaaga tgaacattca gttggtttaa gagaaataat agatataaaa
     7021 catggaggta ttgaaaaata tggcatagaa tgccactatc tagggacttc tgtacctgta
     7081 gaaaaattgg tagatgcagc gattgaactg aatgcagatg caatacttgc atctacaata
     7141 ataagtcatg atgatattca ctataaaaac atgaaaaaat tacacgatta ttgtgtagaa
     7201 aaaggtataa gagataaagt aatgattgcg tgtggaggaa cacaagtaac acctgaaata
     7261 gctgttgaac aaggcataga tgctggattt ggaagaaaca gcaaaggcat acatgtagcc
     7321 actttcttag ttgaaaaaag aagagaaatg agtaataaat aatggaaaat agcaaggttt
     7381 ctttaaaaat agatgtgttg gtagctgaaa ttggaagtac tactacagtg gtaaacgctt
     7441 tccacgatat taatactaac aatcctatat ttttgggtca aggtcaagct ccaacaacag
     7501 tgtttgaagg tggcgatgta agaaatggct tatcaggtgc tattaaagat ttagctaata
     7561 aattatctgt agatgatata aagtataatg atatgtttgc aacttctagt gcagctggtg
     7621 gattaaaaat gactgttcat ggattagtct atgatatgac agtaaaagca gctaaagaag
     7681 cagctttagg ggctggagct gttattcgtc aaataacatc aggtagaatt aaaagaactg
     7741 atttaaataa aataaaagaa ataaacccaa acataatttt aatagctggt ggagtcgatt
     7801 atggagaaag agatacagct atttataatg cagaaatgat agcttctatg aatctaggca
     7861 taccagttat atatgctggt aatgttgaaa atcaagagga aataaggtta atatttgaag
     7921 atactaatta taagctttat ataactgaaa atgtataccc taaaatagat ttattgaata
     7981 tagaacctac aagaagaatc attcaaagtg tatttgaaga acacataact acagcaccag
     8041 gaatgaaata cattaaagaa atggttaatg aaaatattac tccaacccca ggagctgtaa
     8101 tggaagcttc taaactttta tataaaaata ttggagattt attaactctg gatgtaggtg
     8161 gagcaactac tgatgtacat tctgttacag atggaagtga ctatattaat aaaattttgg
     8221 ttaatcctga acctactgca aagagaagtg ttgaggggga tttaggtgta tatgtaaata
     8281 tgaagaatat agtagaagtt ataggtaaag aaaacttaca aagtgaacta tctatagata
     8341 ttgatgcagt aatagagaat tatcctccaa taccaaaatc aaaagaagaa atattatttg
     8401 ttgaaagatt gacaaaagaa gctgttgtta aagctatgct aagacattca ggaaaaataa
     8461 gaaatatcta tggaacaact ggaaaagtca aaattgcaga agggaaagat ttaacagaag
     8521 taagatatat agttggtaca gggggagcac ttactaggct ccctagtaga attgaaatat
     8581 tagataatat gctcaaatac aataaaaata atgaattact atttccaaag gaaaaaacaa
     8641 agatattaat agataatgac tatattatgg catctatggg tgttttatct aaaaaatatg
     8701 aagaagcatc tcttaagctc ctgttaaaaa gtttgaattt tgaggaggaa agattatgta
     8761 tcctaggtta gaaatagata tagaaaagtt aaagcataat gcaaaattaa tttcgcagat
     8821 gtgtcatgaa agaaatatta agatatcttt tgttactaaa tcattttgtg cacaaaaaga
     8881 aatagtagaa gaaatatgta gtgaaggaat agaccatata gcagattcaa gaatacaaaa
     8941 cttaaaaaat ttacaagata taaatctacc taagatattg attagaatcc caatgttaag
     9001 tgaattagaa gaagttatca attattgcga tattagtttt aactcagaat taggaacaat
     9061 aaagaaatta aatgaattat gtgaaagtaa aaatattatt cataagatag ttcttatgtt
     9121 tgacttaggc gatttacgag aaggatactt ttatgaagaa gactttttta ataatgtaag
     9181 agagatagta agattaaaaa atattgaaat aattggtata gctacaaact taacctgtta
     9241 tggagctatt ataccatcta gggaaaatat aggcagactg gtaagtatag caaaaagaat
     9301 ggaagaaaaa tttggaataa atctagaaat agtatctgga ggtaattcaa gttctattca
     9361 tctgttgata aataataata tgccagaagg tattactaat ttgagaatag gcgagtccat
     9421 tctacttggt agagaaacgg catacggtga aaatataaat ggaacatatc aagatgcatt
     9481 taaattaata tgccaagtag ttgaatgtaa agaaaaacca tcagttccta ttggagaaat
     9541 aggagttgat gcctttagaa ataaacctgt ttatgaagat aagggaatct taaaaagggc
     9601 aatcatagct ataggtaaac aagatataaa tattgactct ttaattccaa tagacactga
     9661 tataaaaata ttaggggcaa gttcagacca catgatacta gatgtaagta acacaaaata
     9721 tgattataaa ttaggtgaca atttagaatt tttattaact tatgggggta tcatgtcatc
     9781 tagtacgagc aaatatgttt caaaaaaaat tatagtgtag tgtttaaata attaggagga
     9841 aactatttta tgaaagagcg agaatgtaga aaagcaagtc tgtttgatgc aatgatacca
     9901 attacttgtt tgatattatt tttaagtcta ggagtattag tgtatggttc atcacctcat
     9961 gtaccactaa taggagcagc agcagtagca ggacttgtgg ctgtatatag acttggattt
    10021 aaatggaaag agttagaatt aagtatgttt aatagtataa agatggcaat gcaagcaatc
    10081 ttaatcataa ttataatagg cgtacttata ggaacttgga tagtaagtgg agtagttcca
    10141 tgtatgattt actggggact aaaaatttta tcaccaaata tttttttagt agcatcaaca
    10201 ttagtctgtg caatagtatc tcttgcgaca ggttcatctt ggagcacaat gggtacagta
    10261 ggagtagctc ttttaggaat tggtcaaagt ttaggtatgc caattggatt aatcgtaggt
    10321 tctataatat ctggagctta ctttggagat aaactatcac ctttatctga aacgactaac
    10381 ttagctccag caatggcagg aacagatttg tttacacata taaaatatat gctatattcg
    10441 actataccat cattattaat ttgtttagta atttatggtg taattggaat gaagtattca
    10501 ggtcaagcac tagatataaa acaaatagaa ttaattaggt ctacattaga ttctacattt
    10561 aatactttat caccagtatt attattagct ccagcaatag taattggatt ggttgtattt
    10621 aaagttcctg ctataccagg gcttatagta ggtgctgtat tgggagtatt gtttgcagta
    10681 gtttttcaag gagaatctat gaatacgata ttagatgcag cacaaaatgg atatgtttcg
    10741 tcaacacaaa tacaggaggt agatgcttta ttatctaaag gtgggattat gaatatgatg
    10801 tccactgtat ccttaacaat atgcgcttta tctcttggag gtattttaga aaaaacaggt
    10861 atgcttgaag tagtagcatc ttctttacta agattagcaa agggtgtatt tggaacagta
    10921 ttatgtacta tggtaacttg tacaattacc aatataatag caggtgaaca atatctatct
    10981 atagttatac caggaagaat gtataataag gaatataaaa agagaggtat acatcctaag
    11041 atgttgtcga gagctttaga agatagtgga actttaactt ctccattagt accatggaat
    11101 acatgcgggg cgtatataac agcaacatta ggtgtatctg cactaacata tggtccatac
    11161 gctttattaa atattataaa tccaatagta tctttagtat taattgcatg taagtttaaa
    11221 atagcaagaa tagaagatga acctgaaact tgtttaaatt ttgatgtttg ataatagata
    11281 acttatgtaa gaacgtagga actagggggg tagcctgtgg ggaattagta agacatattt
    11341 tagtgtgcga aattctataa atcagtaatc ttgtgacttt agccatggga ggttcagatt
    11401 agtaaattta tagttttata ttagtctata ttttgatatt ttaattgttt gtataaattt
    11461 tttatagaaa aagatttctt ttagacttgc ctaaaaatta ggcaagtctt ttaagtatgt
    11521 tctgattata agagcactgt gtgacaagaa tgatgagatt atttttgcta tactttcgat
    11581 ataagaaatg gaaggaggtt atgttttata ttcatatgaa ataattagtt acacagtgaa
    11641 ttttcgtata atttggtata aaaagattag aaatgaaaat aaagtaatgg aggaataaaa
    11701 tgactagaat atccaatata acaatagctg aacaatctga gtactgtttt ttggcaatac
    11761 gaaaaacaat tgactttatg gtagaatttt cagagttttc aaaacaaagt tttgaaaaaa
    11821 tttctaaata tctggaggag agaggaattt tatcttcagg agcaccgatt gtatgttttc
    11881 ataacatgga tttagtaaaa ttggatgttg aagtagggtt tcctgtagcc acttatatag
    11941 atgggaaaaa tgagattcta caaaagattg ttcctgcaca gaaaataata actgcaattg
    12001 atttaggacc ttatgaatta caagacccta ctttagaaga cttattttca tgggcaaata
    12061 gcaatggata taaacttcat ggcgatatat attaccagta tttaaatgat atcaatcgtc
    12121 cagctagtga atatcttacg aaaatgatgt tacccattat atagaaagtt aaagattatc
    12181 gtttgttagt tgataattag aagaacattt actgctacta cataaatttt atcctaaata
    12241 atatcaaggt gatttaattt atgtattcta atgtaacccc aatatattgt taaggtctac
    12301 ttaagatgct caaaaggcta tgcttaattt attgttatat aatttgtaga gtggtataat
    12361 ttagaggtaa tattatatag atatataggg ggaaaatatg aaaatatatg atatattaac
    12421 acgtttttat attcaagata tagaaaaagc catacctttt tatgagaatc ttttaaaaga
    12481 aaaatgttct ttgcgttttt cctataaaga agttgggttg gaattagcgc aaataggaaa
    12541 tgttttacta ttatctggtt ctgatgatgc tcttaaacca tttatagaaa caaaatctac
    12601 ttttatggtt gattctgttg atgaatggag aagttatcta ttggataatg gagcagttgt
    12661 agtaagagat aagaaaaaag ttcctacagg atataatatg acattgagac atccagatgg
    12721 tacaattata gaatatgttc aacatacaaa gcaggattag ttaaaaacag attttactac
    12781 tgagcaacta attagaatct attcttagac gtacaattta ttatttttgg tattaataga
    12841 ttataagtca aattataata tttcaaaata gaatagaaat ttatttacat aaaagagagt
    12901 gtttaaaaat gaacttttta gttcatgaag cactcttttt tgtatgaaat caaatacatc
    12961 aatatataaa ataaactgat gaagagctta aattaaaata aaaagattca taacaggacc
    13021 ccattaaact ttaagatagg gtaagtttgt aatcatatta aataattgta aaaagctgtt
    13081 tttagtcgta taatataaat atttatcatt taattaagga atctaataag aagaaaataa
    13141 taaataactt tttagattta ctaaatgaaa agttattgaa aattaaagga ttagagtctt
    13201 taatcagaaa gtaaaaagta atatattaaa tttatgaatt gaaaacttaa acaacaaatt
    13261 tgtttataaa atagcaagat tcattaaata taactaagta ataattgata taatgttgca
    13321 tggataaaag gagggaagct gcaatgtctt acaaagtaat taatgtcata actgctattg
    13381 actatataga ggagcattta tcagaaaagc tggatttaga tatagttgct aatgctgttc
    13441 attactcaaa atatcacctg catagaactt tcacaacttc tgttggccta acaatgcatg
    13501 attacataaa gcggagaaag ttgacagaag cagcaaaatt attggtattt tcaaagaaac
    13561 caattattga aattgcattg atagctggat atgaaagtca acaggcattc acttctatat
    13621 ttaaagctat gtataaaaaa tctccaaata aatataggaa agaacaagaa ttttatcctt
    13681 tgcagctaag atttgttttg aaaaaagata acctattttc aaagaatgta ctagagttgg
    13741 aaaaagaaat taaacttgca agtatgtctg acataccatt atggatgaat ttagtgagat
    13801 tagtcataga tggatttccc aatttgcaag aggaagaata tatatatcaa ttagaacagt
    13861 atattttaga agaaagagct ttaatattaa aacttaataa tgtcgctatt gcaaatatgg
    13921 tgtttaatag agaaactaga agtattgatt tttttggaat acatccacag tatcgtaatt
    13981 cagacattgc acaagtgttt ttgaagaaag ttattgaaga tttcttgata gatacagata
    14041 tttcaattac tacctttcga gagggtgaca aagctgatac aggacataga gatatgataa
    14101 agaagttggg ctttgcagaa gcagaattgc ttgtggaatt tggatatcct actcaaaaat
    14161 ttattcttcc tttaagttaa atagcaagaa tgattaaaaa ctccaaaaca caattaagta
    14221 taatgattca gtgtaagtac ttaattaaca aaggaggatt taaatgtctg aaaataaaca
    14281 agtagtaaat tgcgatatta ttgcatctaa ttctaaacat aatatgtgtg aatatgatgc
    14341 agagttatta actttttttc cagatgagat tgcacatcta atagagataa ataacaagct
    14401 agacaatgct tttaagaaag ctgaaaatct tgttgacaag cttgataagg attatatgga
    14461 tgctaaaatg tatatggtaa aaaatcgtgg tgaaatagac ccacatgaaa tgtttcaaaa
    14521 tgaacaagga ctaaaacaaa tagataacta tggggctttt atggtaaaag ttcgagataa
    14581 gattaataaa atcaaagatt ctccatactt tgctagaata gattttaggt tgaaagatat
    14641 ggatgatgaa tctaagtatt atattggtag atttgcattt gattatgaag atgagcttat
    14701 aatacttgac tggcgttctc caattgccag tatgttttat gattatgaaa tcggaaaagc
    14761 tggatatgat gctccaatag gatgggtgga tggagaaata acaagaaaac gacaatttaa
    14821 aattaaaaat ggaaaattag aatatgcact agaaagttcc attaatatac aagatgatat
    14881 tcttcaaaaa gaacttagtc acacttcaga tgaaaaaatg aagtcaatca tttcaacaat
    14941 acaaaaggaa caaaatcaaa ttattagaaa tgataaagct gatacactga ttattcaagg
    15001 ggtagctggt tctgggaaaa catctatagc attacacaga attgcttttc ttctttatcg
    15061 atttaaagat aaaatatcag caaataatgt aattatccta tcacctaata aagtctttgg
    15121 agactatatt tccaatgtcc ttcctgagct tggagaagag ccattatgtg aattaagttt
    15181 tgaaaatata gcagaggtac aattagatag agttattaat tttgaaagtg agaaagaccc
    15241 attagaaata aatgatgcta aatggtctga aagagtgcgt tttaaatcta cacttgattt
    15301 tgtaaaactt attgatgatt atataaaaca aatgccaaat aagattttta ttccaaaaga
    15361 ctacactttt ggaagtttta cagctaaaag tgactggata cagagtagat ttgaggcata
    15421 taacagatat ccagttaaga agagattaga gaaggtagct gaagacattc attataaatt
    15481 tgaatcagat aatattatgg aagaagattt accaaaggta aaaagtatac ttaaaagttt
    15541 gaatggaatg ttgacaataa aaaatactct tacactatat aaggatttct ttaaacaaat
    15601 gaatatatca aacatgtttg tgatggcaga aaagaaaaca cttgaatggt cagatgtata
    15661 cccatttata tatattcatg ctgcatatga aggtatacaa gaagataaga ttataaggca
    15721 tattgttatt gatgaaatgc aggactatac acctattcaa tatgctgtaa taaacttact
    15781 ttttaagtgt aaaaagacta ttcttggaga ttttggacaa ttggttaatc ctaatcatac
    15841 tcatacacta gatgatatga aacagctata taatgatggg gaattagtta tgttgaacaa
    15901 gagttaccgt tctacttttg agattattaa tttcgccaaa aagattcaag acatatcttc
    15961 attagagcct attgaacgac atggtgaaga accagcttta ttaaaatgta ataacgagca
    16021 agatgaaata aataagataa aaatagaaat tgaagaattt aaaaagagtg ataatgcgac
    16081 tcttggtatt atattaaaga cagataatga cgctaaagct gtatatgatg cgttaagaga
    16141 agaatatagt gttcacttaa tatcttctga aagttctagc tttacaaaag gagtatccat
    16201 aacttctatt aaaatgtcaa aaggtttaga gtttgatgaa gttattgtac cttcagtaaa
    16261 taataaaaca tattatagtg attatgaccg cagtttgtta tatattgcct gtacacgagc
    16321 tatgcataaa cttaagttga catatacttg tgaattgact caacttatag atatgtagat
    16381 tatatactta ttatgtaagt gtttacttat ataaatataa cctgtcaata aaattcatta
    16441 ataaatcaat aaaaacaata tttgtaaaag taaaagctat aaattatata ttaaatttta
    16501 taatttatag cttttataat actgcttatc ttaatcctcc caatagcaaa ataattttaa
    16561 acatgccata ttttataaat gtaataacaa ttatacaatt aaaataactt tcctaaacta
    16621 acttgacatt taaaacttac tattgtaata tttaaatatt acaatagtaa gttttaaatg
    16681 tggaatcaat tattatatca caaattaaaa ggaggacagg atgttattaa tagttaccta
    16741 attatcttcg caataggttt aatcaacata attgtactag gtgaagaaaa tataagagat
    16801 acagctattc atttagtctt gtttcttata agtagaacct agattggagt atattacaaa
    16861 taatgtctac tattttaata gtaggttata aatttaaata tgggagatga tataaattat
    16921 gaaaagaaaa aagaatttta tttggatagc tatcttattg gttggtgtag ttatggtaat
    16981 gtattatgta gggaaaaaac ataacgatat aaatcaaaaa actgataaga attatataaa
    17041 atctgaatta aataagtctg aattaaataa atccaaaatt gaaaacaatg ataaaaacaa
    17101 aaaagaaaaa attaatatgg ttgactatag tgattgtttt gaaggtatta gtggaggagc
    17161 tattttttat aatactaaaa ataaagaata taatatatat aacaaagaat taattgaaac
    17221 aagaaggtca ccatgttcta cttttaaaat agtttcaact ttaattggtt tagaaaaagg
    17281 ggtaataaac tcaaaagaat cagttatggg atatgatggt acagagtatc caaataaaaa
    17341 ttggaataaa aatcttagtt tagaagaggc atttaaggag tcttgtgttt ggtattacaa
    17401 aaaattgata gataaagttg atgctaaatc tgtccaaaac attttagatg atttaaagta
    17461 tgggaattgc gatattagcg aatgggaagg cgatttaaaa aatggaaagg gacatttaaa
    17521 tggattttgg ttagaatctt cattacaaat atctccaaaa gaacaagtgc aaacaatggc
    17581 aaaaatattt gagggagata caaactttaa aaaagagcat attaatattt taagagatat
    17641 tatgaaaata gatataaatg ataaaaacat aaatgtatat ggaaaaactg gaacaggatt
    17701 tgatgaaaaa aataaacgtg tggatgcatg gtttgttggt atgctagagc gtgaaggtga
    17761 cacctattat tttgctatta aatcagatga ttccaataaa gaaattacag ggccaaaggt
    17821 aaaggaaatt gcaattaata taattaagaa atattatagt gtaagggaat aaacttaatt
    17881 ttatttgtaa atattattta cctatagtct gtaatattta ttaaataat
//
LOCUS       DAMKMI010000083        17720 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMKMI010000083 DAMKMI010000000
VERSION     DAMKMI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.084"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            78..413
                     /locus_tag="PC429_002440"
     CDS             78..413
                     /locus_tag="PC429_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3847941.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            550..1827
                     /locus_tag="PC429_002441"
     CDS             550..1827
                     /locus_tag="PC429_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF3847942.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKEQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1948..3336
                     /locus_tag="PC429_002442"
     CDS             1948..3336
                     /locus_tag="PC429_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3847943.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRVLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3540..4253
                     /locus_tag="PC429_002443"
     CDS             3540..4253
                     /locus_tag="PC429_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF3847944.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVGLSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4371..5240
                     /locus_tag="PC429_002444"
     CDS             4371..5240
                     /locus_tag="PC429_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF3847945.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANINLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKELVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5395..6540)
                     /locus_tag="PC429_002445"
     CDS             complement(5395..6540)
                     /locus_tag="PC429_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF3847946.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDIDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(6873..7649)
                     /locus_tag="PC429_002446"
     CDS             complement(6873..7649)
                     /locus_tag="PC429_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF3847947.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8231..9205
                     /locus_tag="PC429_002447"
     CDS             8231..9205
                     /locus_tag="PC429_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF3847948.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9303..9608
                     /locus_tag="PC429_002448"
     CDS             9303..9608
                     /locus_tag="PC429_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF3847949.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9764..10924
                     /locus_tag="PC429_002449"
     CDS             9764..10924
                     /locus_tag="PC429_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF3847950.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            10974..11876
                     /locus_tag="PC429_002450"
     CDS             10974..11876
                     /locus_tag="PC429_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF3847951.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            11956..12849
                     /locus_tag="PC429_002451"
     CDS             11956..12849
                     /locus_tag="PC429_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF3847952.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      12902..13083
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13311..14441
                     /locus_tag="PC429_002452"
     CDS             13311..14441
                     /locus_tag="PC429_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3847953.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14513..15538
                     /locus_tag="PC429_002453"
     CDS             14513..15538
                     /locus_tag="PC429_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF3847954.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15541..16737
                     /locus_tag="PC429_002454"
     CDS             15541..16737
                     /locus_tag="PC429_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847955.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFNLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(16865..17590)
                     /locus_tag="PC429_002455"
     CDS             complement(16865..17590)
                     /locus_tag="PC429_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF3847956.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
BASE COUNT         6800 a         1978 c         3087 g         5855 t
ORIGIN      
        1 gtatatttat aatattaata aatatattgc tataaaagtt tgtttaataa attaaataaa
       61 gaaaagagga gaataaaatg tacaacatag atgatatgat atataattgt ccagtagaag
      121 ctttatcaaa tattttaggt aaaaaatggg tggctataat tatatggaaa atacaagaag
      181 ataaaaaacg atttggagag ctacaaagag ctataccaga ctgtagtaag aaaatgttag
      241 ttcaacaact agatttttta attgaacagg ggattatagt caaccaaaaa aagttggtta
      301 ataatgttgt agagtcaaca tattttttaa gtgaatcagg attacgattg cttcctgtca
      361 tggaaaaaat gattatgtgg agcaataaaa atttaaattg tgataaatac taaaatttac
      421 tctgtaagta atgaaaattt aattaagcta tgagtagaaa aatgtattaa aattacataa
      481 ttttttagtt agattttaat ttagaaatgg gattgtaata ttggaaataa aataaggagg
      541 aattttaatt tgcaaaatgt aaatttattt ttagtattta tagaaggtat agtatcattc
      601 ttttcaccat gtatactgcc aattttgcct atttatttga gtatattgtc aaatagtagt
      661 gtagaaaatt taaaagaagg aaagacgagt tttataggaa gttctttatt taaaaatact
      721 atattttttg ctcttggaat ctcaacaaca ttctttatat tgggttcatc agtaaaagta
      781 ttgagtatgt tttttaatga aaataaagat ttaataatgt ttattggtgg gattataatt
      841 attatcatgg gattatttta catgggaata attaaatctt caatattgaa tagagaaaaa
      901 agatttaatg ttaagtttaa agaaatgaaa gcaataacag cttttatatt aggttttacg
      961 tttagttttg gatggacacc ttgtattggt ccaatattgg catctgtatt agtcatggtt
     1021 tcaagttcaa gtaatcatct tagtgccaat cttttaatag cagtatatac tattggtttt
     1081 attttgccat tcataattac agctatgttt tacagtaaac tatttaaaac tatagataag
     1141 ataaaatcta acatggaaat tattaaaaaa ataggtggga ttatacttat agtatcagga
     1201 atattaatga tggtaaatgg atttggaagt ataagcaaac actttaatac gtcacaaaat
     1261 agtaaaattg aaagcaagca agaagaaaat aagcgagaaa attctactga taaagaagaa
     1321 aattctgatg gtaatgatag tcaaaaagac tctaataatg acaataatga taaagggtct
     1381 aatgatgaag atagaataaa gtctattgat tttactttaa cagaccagta tggtaaaaca
     1441 cataaattaa gtgattatga aggcaaagtt gtattcttaa atttctgggc tacatggtgt
     1501 cctccatgta aagaagaaat gccttatata gaacaactgt ataaagacta taataaaaat
     1561 aatgatgatg tagtaatttt aggagtagct tcacctaatt taggtagaga aggttcaagg
     1621 gaacatgtag ttaacttctt aaaagaacaa ggttatacat tccctgttgt acttgatgaa
     1681 gatggagcat tagcatatca atatggaatt aatgcttttc caactacatt tataattgac
     1741 aaggaaggat atgtaactca gtatattcca ggagctatgg ataaggcaac tatggcatca
     1801 tttattgaaa atcagagaaa taaataaaag tatttaagta taagtttata aatcaattta
     1861 aggttacaaa aaagtgccta attgtaccac ctaaataatc ttgttatagt aaaagtataa
     1921 acaatacaag aaagggttga attttatatg aaaaaaacat ttgatgctat aataatagga
     1981 tttggaaaag gtggaaaaac attagcaggg gatttggcaa acaggggatt aaaagtagct
     2041 ttaatagaaa agtcaaacaa gatgtatggt ggaacatgtg taaatgtggc atgtatacca
     2101 acaaaaagct tagaaaattc agcaaacagc gtaaaaacaa agaatataaa ttcttgggat
     2161 gaagtacaag ctgaatatga gaaagctata gataaaaaag aaactttaat tacaaaactc
     2221 agagaagcta actataataa attaaattca aatgagaatg taactatatt cacaggaatg
     2281 ggaactttca ttgatgaaaa aacagttcaa gtcaaaactg aaaatgaaat ttatgagtta
     2341 gtagcagata atatatttat taatactggt tcaagaccat ttatacctaa tattaaggga
     2401 attgaaaata agaatatagt atatgatagt gagtctctta tgaatctaag agttttacct
     2461 aaaaaaatga caattatagg agctggattt ataggtttag aatttgctgg tatttatagt
     2521 tcatttggag cagaagttac aatattaaac tctaataatg gcattttacc aaatgaagat
     2581 gttgaggatt cagaagaaat aataaaatta cttgcaaaga gaaatgtaaa aattgttaat
     2641 aatgcaaata taaaagagat taaagaagtt tctgaattag caatagtaga atatgaagta
     2701 gatggtaaat ctaaagaatt aacaagtaat atgatacttg tagctacagg aagaaaagca
     2761 aacactgaag ggctagggct agaaaatgca ggtatagaat taaatgaaag aggatttatt
     2821 aaagtctcag aaactcttaa gactaataaa gagcatatat gggctatagg agatataaat
     2881 ggaggtccac aatttactta tatttcattg gatgattatc gcatagttat taatcagtta
     2941 tttggagata agactagaac aactaatgat agaaagaata tcccaaattc tatatttata
     3001 agtccagcat tttcaagagt tggtcttaat gttaagcaag ctaaagaaaa gggatatgaa
     3061 gtattagtgg caaagatgcc agtagaagca attccaagag ctaaacagat tggcaaggca
     3121 gatgggttta taaaaatagt gatagataaa aaatccaata agatacttgg agctagtatg
     3181 atatgtgaaa attcaagtga gattatccat cttattcaat tagctgtaga tttagaagta
     3241 gagtatacat atttaagaga ccgtgtttat gcacatccaa ctatgacaga agctcttaat
     3301 gatattctat ctcctaatat gataaaagaa gtataacata agtttaatat ataaatctaa
     3361 aattatcatt ataagtagat tttagaaaga ggctatctca aaaaatagag ataagtctct
     3421 tttttactgt ttaaataata aaaatatatt ttaatggtat taaaaattat cttaaaaggt
     3481 gaaataatct gtatttaatg tagtacagtt atatctttaa agcaaggtgg tgaaatgata
     3541 taaataagat atctaaaaga gggtttgatg atagtgataa aagatggttt tcaagtaaag
     3601 aattaacaag acttataaag gctaaagaag aaatagaatg gttattaaat agggattaca
     3661 aaatagaccc agtcgttact tttgttggtg atagatatca gttttctata agacaaagag
     3721 atgcattaaa aagggcagtc tgtactgatg aaaaaaatat tataagacaa tcaaagaggc
     3781 tttctctaga taaaatcaaa gaagataccg taaatataga tggctttaat ttgataatat
     3841 ccatagaggt aggattatct ggtggtactc tagtaatagg aagtgatggc aatataagag
     3901 atttagcagg tcttagggga acctataaga ttatagataa aacagaagaa gctttgaatc
     3961 tgataggtaa attttttaat aaatacaatg cacagaatct taaattttat ttagatgctc
     4021 cagtttctaa ttcaggaaat ttaaagtata ggatattaga gcatgccaaa acttggggaa
     4081 ttgaaactga agtagaatta gtaaaaaatg cagatgttgt attggaaaaa ttagatagag
     4141 tagttagtag tgatgcagtc atagttgaca agtgtataag ttattttaat gtggctagag
     4201 gtataattga agagtatatt aaagaatgta atatcataaa tttaaataaa tagataaata
     4261 taggtgagaa tatataagta tatgtagcaa tattcttagt aatacaagta aagtttgaaa
     4321 ataggagaac gaatacatat cgaatttcat aaaatgttgt aaaaagacat atgagaggat
     4381 ggatatgtgt gaaattaact aaactaaata ttaaaaaata tagagcacct atatataaat
     4441 atgctttagc aaatataaat ttgaggtctg ctaaatctac caattcaagt atcataacag
     4501 taatacctca gggagccaaa atggaagtat tggatgagga agatgactgg attaaggtga
     4561 tgtacaattc tcaagaagga tatgtgtata aggagttggt atcagtaagc gaatatgctt
     4621 ggagtaactt aaacttaaga gaagataaat ctacaacttc caatataatt acagtaattc
     4681 ctgaaaaatc tagggttgaa gtccttcaag tagatggaga ttggagtaag gttgtatatg
     4741 atgataaaat aggatatgtt tttaattatt ttttatcaat tgatgggaat aaaccaaatg
     4801 aattagatta taagtatttt tatactgaca tgattaaatt tgttaacgaa aataatatta
     4861 aaagtactag cgattatcta attgtaacag accttagaaa taaatatact tatattttta
     4921 agaaagataa tggaggatgg ggacaacttt acaagtggca atgtactata ggcaagcctg
     4981 agactccaac tataacaggt atattttata taagtggaag aaaaccttct tttggaacgg
     5041 atgagtattc agttaagtat gctactagaa ttaaaggtgg atattactat cattctgtac
     5101 tatatgattc aacaggaagt tatataatag atggcagact tggtgaagct cttagtcatg
     5161 gatgtataag attaagtacg gaaaatgcaa agtggatata tgataatata ccagatacaa
     5221 caacagttat aattcattaa gaaaaatttt gagtttttta ttgggataat gattcttaaa
     5281 ttaataggat tatatattaa cttctcataa agagtactat ttaaaaagtg taataagaga
     5341 gtgctattaa caaaacaaag atattttgag gcactctctt ataaatatac ataattaaga
     5401 tattttcata aacaattcta taatcttatc tttatcaaag aaaacaggat tagcaccagc
     5461 acaagcatct ttcatagagt tttctgcaag caattcaaaa ttaggattat caacacctac
     5521 atcttttaaa gattttggga ttccaacctc ttcagaaaga gcttttatct tatctattac
     5581 aaagtcaaca cactctttat ctgatttatt atttacatcc attccaataa cttcagctat
     5641 tggtcggaat ttggcagggg ctgatttagc attttcctcc tcaactattg gaagtagcat
     5701 ggcattacat actccatgag gtaaatcata taaacctcct aattgatgag ccatagcatg
     5761 tacatttcca agacctgcat tactaaaggc aattccattt aaaaaacaag cataacacat
     5821 ttgttctcta gcttctatgt cattaccatt tttaacagct cttggaaggt atttaaatat
     5881 ctgctttata gcatataagg cagttgaatc tgtaacatca taagcaccat tagctactac
     5941 agcttctata gcatgagtta gtgcatccat accagttgct gctgttaaat tagatggttt
     6001 gctaatcatt aattctgggt catttactgt cattgtggct agtgaattgg tatcaaccat
     6061 tatcatcttt acatgtctgt cttcatcagt tataacatag tttattgtaa cttcagcaga
     6121 tgtaccagca gtagtagtta tagctatgat aggaagacat tttttagaag ttttgttgat
     6181 accttcatag tctctaatat ctcctccatt agtggctaat atagagatag ctttaccaca
     6241 gtcttgtgga gaaccacctc ctatagttat aacgaagtcg caattttctg attttaatat
     6301 tttaagtcct ttattcacat tagttacagt tggattttgt ttaacttcat cataaacaac
     6361 aaagtctata tctatctgag ataaaatatc tgttactttt tttacagtac catttgaaac
     6421 tagaaattta tcactaacaa taaatgcttt tttagttcct aatgatttaa ttgaatcttt
     6481 agcttcatta agacatcctt tcccaagtaa atttattggt ggtacataat aaatgctcat
     6541 aactaaaatc tccttttatt ttcattgatt tttatgtaaa aatatatatc taacaatttt
     6601 cactaattta ctcataatat atcgtatctt agattgagat agggttgctg gttcttatat
     6661 aagaacttaa ttcgtaatta cgattatgat tagaatttaa catagtattt ttatcatgtc
     6721 aataaaaata taaaaaatat tataaaaaaa acaaagttac tgtagttaaa tcagcaactt
     6781 tgtttttata gtataaatca aataaataca ttagtttaaa acaaaatttt taattattat
     6841 gttttgatat cttccatata cagaatatta atctaattta ttacaatgtc ttcattacgt
     6901 tcttccatca ttgattgaat atttttctga gcatttaaga gtaatttttt tatcagttct
     6961 attttttcgt cattagctct aaataaaggc agtgttacac taacagcagc tcttatacat
     7021 ccattttttc ttattggtac ggcaagacat tttaggtgtt ccattgattc ctcacactct
     7081 gaagctattt gtgttctttg aacttcaaga agctggtcgt ataaaacatc aaaatctgta
     7141 attgattttt ctgtaaaacc ttttagacca tctggatata agcttattag tttttccttg
     7201 tcaaaatcac ataaaagagc ttttccaaga cctgtacaat tagcaggtat tttttttcca
     7261 acataagaaa taagtctaat cggttctgga gaatctactt ttccaatata aagaacttca
     7321 ttattgtcaa gtataccaag ctgacaagtt tcagaacagg aattaactat attagtcatt
     7381 tcgtcaatta tatagttaaa aatagttctt ttttcaaaaa atgaagaccc taatgtgtaa
     7441 gaacgtatcc ctatagaata tttggatgtg tttttatcaa tagtaatgta ttttctagca
     7501 ttaagtgtat gaactatagg aaagatgcta ctttttggag cattgataac atctgctatt
     7561 tctgtaagag tatatccatt ttcggattct gctaatgctt ctaaaatatc taatatccta
     7621 gctgttgggc gatgattatc tgaagtcata aatttccccc tctttttatt aatttaaagt
     7681 attagaatat gttgatttta gcacactata cgatatttta caatgtagaa aataaatatg
     7741 taaatttata tataaataag ctcttgattt agatacagat agatattttt ttagactgta
     7801 cattaaggaa taactaattt ccaaaatata cctaaagaaa gtaagtgata tttagttata
     7861 taatttaagt aaatatatgc aatagttatt tttatatgta aatatcaaaa tattatgaac
     7921 ttatatttta tctatttttt acactgtata aaaactttat agttttgaat actatataat
     7981 tttaaatacg ctgtattttt gcatataagg aagtcatttg ataatataca aggtattatc
     8041 agaaagtttg aaaaaatttt gacaaaaatt tatttttttt attgactcat aaataggaaa
     8101 atgctatcat taattcaaag attcgtaatt aaaaacttag ttcttaaata cgaaccgaaa
     8161 cggccgttag aattaaagct aaaaacttaa taagagtaat taaatatcat gttttaggag
     8221 gattaacttt atgataatgg attttattaa aaaaattcct gcaggtatga tgatagtgcc
     8281 aatgtttata gcagctttca ttaatacctt ttgcccagat attgtacaaa taggttcgtt
     8341 tacgacagca gtattttcaa gtgttgggtc tgcaactata attggggttc aactggtatg
     8401 cttgggaact cagcttcaat ttagagaaat gccaaaagtt ttaaagagag gtggtatact
     8461 tcttggttct aaatttgcta taggtgcttt gataggtata cttataggaa agctatttgg
     8521 aatggatggt gtccttggtc tgacaacatt ggctgttata agtgcagtta caaatagtaa
     8581 tggtagtata tatctttcat tgatgacttc atatggagat gaaacagatt gtgcaactat
     8641 gagtttactt actttaaatg atggaccatt ttttacatta atagctcttg ggacttcagg
     8701 tcttgcaaat gtaccattgt tgtcattatt ggctgcaata gtgccaatac ttgttggtat
     8761 gataatagga aattttgaca aaaaaatgaa agagttttta gagccagggt gtaatttact
     8821 cgtacctttt gtagggtttg cacttggtag tagcataaat cttgcaagta tactaaaagg
     8881 aggtttatca ggagtattac ttggattaat atctgtattt gtaggaggta tatttatagt
     8941 attctgtgat aaatttatag gaaaacgtcc aggatatgca ggatgggctg tagctacaac
     9001 tgctggaaat gcaattgctg tacctgctgc tgttgcatta gttgacccat catggcaacc
     9061 atatgttgca acagctacaa cacaagtggc agccgcagta gttgtcactg caatattagt
     9121 acctcttata actagttggt gggctaagaa gtatggatgt cctaagttcc caaaacaaaa
     9181 tgaggatggt acaataacag cttaatttat attacagtga gttgttggta aaataataaa
     9241 ttctttaatt agattttagg atattaataa aattatatta tttataaagg agagcaagaa
     9301 taatgataaa tgcagtgata attgatgaaa aagataatgt tgcagtagct attgaagcaa
     9361 tagctaaagg tagtgaaatt agttttaaat taaaagacaa aacagtaaaa acgattacag
     9421 cactagatga tataactatt tatcataaat tggctataaa agatatggcc aaaggtgata
     9481 aagttactaa atatggtgaa catataggag aagcaaatca ggaaataaaa agtggccaac
     9541 atgtacatat tcataatgtt agaagtgtta gagaggattt ggataaacaa gttatgacta
     9601 atatataaaa taatatttat agagttataa aagttgtatt cattgctttt agtgtcaaat
     9661 agtttttaaa taggttttta gctgtttgat tagtgagtgt aataaaaatt ataatatata
     9721 aaaattatag aaagtatata gactatttta ggaggaaatc agtatgaaat tttatggata
     9781 taaaagacca gatggaagag ttggtataag aaaccacata ttaatattgc ctacgagtgt
     9841 gtgtgcatca gatacaacta gaataatagc atcacaagtt caaggggcag ttacctttaa
     9901 taatcagaat ggatgttcac aagtacattc agaccaacaa ttaactatgg atgttatggc
     9961 tggttatgca gcaaatccaa atgtatatgg tgtaatagtt gtatctttgg gatgcgaaaa
    10021 ttgccaaaat gatttggtag ttgatgcaat aaaagagaga acaaataagc caataaaaac
    10081 tcttgtaata caagaagaac atggaacttt aaaaactata gaaaaagctg ttcgttatgc
    10141 tagagaaatg gcacaagaag cttctttgct tagaaaagaa gaatttccaa tatctgaact
    10201 tatactagga actgaatgtg gtgggtctga cccaacaagt ggattagcag caaatccatt
    10261 aataggtgaa ttaagtgata aacttgtcga tttaggagca acatcaattt taagtgaaac
    10321 tactgaattt ataggtgcag agcatatact agctaggaga gcagtaaatg aagaagtaaa
    10381 agaaaaaata ttacatatag tacatagata tgaaaattca ttaaaattgg ttggagaaga
    10441 agttagagaa ggtaatccat ctcctggaaa tatagctggt ggtttaacaa gtttagaaga
    10501 aaaatcactt ggttgtatac ataaaggtgg tcatagacaa ataagtgaag tatatgatta
    10561 tgccaaacaa attgataaga aaggtcttgt tataatggat acccctggaa atgatgcttc
    10621 atctgttgct ggtatggtag caggaggagc acaaatagtg gtatttagta caggaagagg
    10681 aacaccatca ggaaatccaa tagcaccagt aataaaaata actggaaata aaataacttt
    10741 tgcaaatatg gaagataata tggattttga tgctagtcca gttatctatg gtccacaaac
    10801 aatggaagaa ttaacagatg atttgttaaa tatggttgta gatgtagcaa atggaaaaca
    10861 atcaaaagca gagtcccttg gatatactga aatggcaata gctagagtat gcaactatgt
    10921 ataatcttta agtaagaaaa taaatacata gaattttaat aaagggtgag attatgaagt
    10981 ttgtaagttt tactgaagga gaaaatgaat gtaaaagaac tggagttttt tctaaagatg
    11041 aattatttat tatagatgtt aactctttaa atctcagtag aaattttaag gatttaaatg
    11101 aattaattca aaatgtatct agtgaagata ttattaagtt gaaaactatt ataaatgaaa
    11161 gtacattcta taattatgat gtttttaaaa gagagagatg tacggtacat gttcctatag
    11221 aaaaaccaat acatgatatc ttatgtgtgg gagtgaacta taaagaccat cttgaagaaa
    11281 ctcaaactca ctttgatgat agttttgaag aaccacaaaa aacggtttat tttacaaaac
    11341 gtgtgacaaa ggcaataggt ccagaagatg agatagatgg tcattttgag ataaataatc
    11401 aactagacta tgaagttgaa cttggagtag taataggtaa aacaggtgtt aatattagaa
    11461 aagaagaggt agaagattat atctttggtt acactgttat aaatgatatt tctgctagaa
    11521 agttgcaaaa agaacatgtt caatggttta gaggaaaagg attagataca tttacctctt
    11581 taggtccatg tattttacat aaaagtgcag ttccttttcc tataaaactt aaagtatgta
    11641 gtagggtaaa tggtgaagaa agacaaagtt ctaatacagg attattcata tcagacgttt
    11701 cagacatagt ttcagaactt tcaaaaggta tgaccttaga ggctggagac attatagcta
    11761 ctggaacacc ttctggagta gggatgggat tttcacctcc tcgatatatg agtagtggag
    11821 atgtagttga atgtgagatt gagagtattg gaattttaaa aaattacatt aagtaactgt
    11881 tttaatataa ttaatattaa agctaaatct gatgtattaa tgaacatatt aaaataaatt
    11941 ttaggagttt aaattatgaa agcagaattt ttgacaccag ttgttacaat atttgatgaa
    12001 gaaggaaatt tagataaaga aggtaataaa aaagtgtatg accatcttat agatggaggt
    12061 gtagatggac tagtcataat gggaagtact ggagagttct ttaatatgtc tatggatatg
    12121 caaaaagaac ttatagattt agtaactagt catgtaaata agagagtaaa agtgtttata
    12181 ggtacaagtc gtatgtgtat aaatgaatct atagaacttt caaactatgc acataatagt
    12241 ggagcagatg gtgttatgat aataagccct tattatttct ctcttgatga tgatagtatt
    12301 gagttatttt atagtgaaat agctaagaat acagaggctg aaatatattt gtacaattat
    12361 ccagatagaa ctggttatga tttaagtcca gaacttacat taagacttgc aagaaaacat
    12421 aaaaacatag ttggatataa ggatacagta actttgatgg gacataccag agatttaata
    12481 aatactatga aaatagagtt cccagaattt aaggtttata gtggttatga tgaaaacttt
    12541 atacataatc taatgtctgg gggagcaggt tgtatagggg ggttatcaaa tttaattcca
    12601 gaagtttgct ccaaatgggc tgaagcttac aataatagag atatggagaa ggttatagaa
    12661 attcaaaaat atattaataa agcaatggat ttttacttaa taaccaatcc atttatacca
    12721 gcaatgaaaa aagctatgaa tatcagaggg ttacaaataa gtgattatat gactgctcca
    12781 tttgtaaaac caaatgaaaa acaagtatgt gaaattaaaa aattgatgaa tgaattaaat
    12841 atatgttaaa tcaaccttgc aaagaggtca acaataaatt acaattatct taataaaaat
    12901 taaattaaaa taatactagg gtactatatt agtttaatag ggaaagtgat gaaaattcac
    12961 tacagccccc gctactgtat acggatacga aatcaaaatc cactgaaatt tataaaaatt
    13021 ttgggaaggg tgatggagga aaagccgtga gtcaggagac ctgcccagta ttatataagt
    13081 tcaatttagg gtggagattg taatttataa taaaatgaag tatcaattcg tttttgtaaa
    13141 gttgatatat gaacttctcc ttattaaatt aggagaagtt ttttatttta ttaaaaggtt
    13201 atacttatta aagatgactt taggataact ttaataagta tactttaaaa agcatatatt
    13261 attaaagttt tttagccact cataaaaaat aagatattgg agtgaaagag atgaaattta
    13321 aatcaaatgt tttaaagcag ttttttttaa ttgctatggt aatttttatg gtaggaggtc
    13381 ttatagggtg tagtaaaagt gacaaagaga ataaacaagc tagtactggt aagaaattag
    13441 aattaaaata tgctagtgga tttagtgttg aagatttaga agatggaatt aaaaaagtaa
    13501 ctgatggaga taatcgagaa ctgattttaa tcccaaagaa gattaaaatt cctgaaaaat
    13561 ataaaaatgc taatatagtt agaacgcctg tggacaatgt attaatagca tctacaacac
    13621 aggcttgttc tctgagaaca atagatgaac tagatagtat aaaagccgta acaacagaag
    13681 agcagtactg gactattaaa gaaattaaag atttaatgaa agatggaaaa atacattacg
    13741 ttggttcaaa tttagcacca gattatgaaa aaatagtaga cttaaatcct gacttaacta
    13801 tagtgagtag tggtttatca gaggctgata caaaatttat tgacaaatta aaagaattag
    13861 ggcttaatta tgttgtagac aatgagtata aagagcaaaa tccatttgga agaatggagt
    13921 ggacaagttt aatagctgca ttttatgata aagaagatat ggctactagt gaactaaata
    13981 aaactgtaca aaaggtagaa gaagtaagta aaaaaataaa aaacaaagct aaacctaaag
    14041 ttgtatgggc atctgtatat gaaggaagtg cttatatagc tcctaaagat tcatatgtgg
    14101 ataatatgat aaaaatggct ggtggaataa atgcatgtgc ttcaatagat tcaaatgaag
    14161 ggcgagtaag tattgaacaa ttgcatgaag ttgcaaaaga tgcagatata tttgtatact
    14221 catcaagttc agattatgca ccaaatttag attctataaa atctagtgca cctgtattgg
    14281 ctgatttgga tgttgtaaaa aacgggaacc tatgggtgtt tgctccagat tattatcaat
    14341 cagtagataa gacggacgaa ttaatagtag atttagttga gatgttccac ccaggtacta
    14401 caggtgaaaa aatcaaacat tatataaatt atgataaata gtacagagag gacacaaaaa
    14461 gataggtttt atagatttta gttaatatag ctttgctaag aggagtatac aaatggattt
    14521 tatcaataaa atgagaataa ggaatacaac aatatttata gtattaatta taagtatgat
    14581 acttactttt ttagtgtgtg tagcatttgg ctctgtaaaa attgaattta aagaagtaat
    14641 agatgttgta ttaagaggta tggataatac agaacattat aaaataatat acaatataag
    14701 aatccctaga gtacttgcaa ccatggtagg tggagcatgc ctagcagtat caggtatttt
    14761 attacagata ttttttaaaa acccaatagt tgaaccgtat atattaggag tatcttcagg
    14821 ttctacttta tcagtagctc tcatgatttt gggaggatac acattgggat ttgagagtgt
    14881 aaattcaatg agtatatttg tagcagcttt tataggagct ttaattgtaa tgtctatagt
    14941 cattttattt gcaaataagg taaagaatat aacaactctt ttaataatag gaattatgac
    15001 cggatatgta tgtagtggta taacaaatgt gttacaatca tttgcaaata gtgataaatt
    15061 aaaagaattt actatgtggt ctatgggaag tttttctgga tttacatggg aaaaggtaga
    15121 aatacttata atagttggta tattaggtct tttgatgact gtattcataa taaaaccttt
    15181 aaatgctttt ttactaggag aagagtatgc aaaaagtatt ggcataaata taagattttt
    15241 tagagttttg atagtattta tatctagtat attagcagca atagtatcag catttgcagg
    15301 acctataggg tttattggac ttgcagtgcc acaaatttca aaaatgatat ttaaaacatc
    15361 agataataga gttttgatac cagtagtaat atttttaggc agtatagtta cagctctttg
    15421 tgatttaata tcaagagtaa ttttatcacc agtagaatta ccaataagta cgataacatc
    15481 tttattagga gctccaattg tagtaatgtt attggtgaaa aggaaggata gggcttagac
    15541 atgttgaaaa ctaataattt aagtgtagga tacaataaca aagttgttat aagtaatata
    15601 aatgtagaag taaaaaatgg agaaatacta tgtcttttgg gaagtaatgg agctggaaaa
    15661 actaccctat taaggtcctt atctaaactt atatcaccaa taaaaggtga aatatatttg
    15721 aatggtgtaa acataaactg tatatcaaga aaagcattat caaaaaaaat ggctttagta
    15781 cttacaaata gattattagg tgatttaatg actgtacaag atatagtcaa tataggaagg
    15841 tatccatata caggattttt tgggagtcta tctaagaaag atttaataat ggtagatgaa
    15901 gcattggagt ctgtagatgc cttacattta aagaaaagat attttgatga actaagtgat
    15961 ggagaaaaac aaaaggtgtt ggttgcaagg gctcttgttc aagaaccaga aataattata
    16021 ttagatgagc ctacaactca cttggatata aaacatagat tggaactcat aaacatactg
    16081 aaaaaattaa gtaaagaaaa gtcaatatca gtaatacttt cattgcatga aatagacata
    16141 gcattaaaaa gttgtgataa agttgctctt ataaaaaata ataaagtcat agcttatggt
    16201 cagcctgaag atgtagtaga tgaggatatt ataaattcgc tttatgaatt ggatgataaa
    16261 aactttaata gtcttttagg ttcagtagaa attagtaata aatctaaaaa tgaagttttt
    16321 ataattggtg gtgggggtaa agcaacacca atttatagag cttttactaa aaaaggtata
    16381 ggtctgtatt ctggaataat acatgaaaat gatattgatt atgaaattgg tagaactatg
    16441 gggataaaga tgtttacaga aaatccattt gagcctataa gtgatgagag tttcaatttg
    16501 gctattagaa atttaaatga ttccaagatt ataatagata caggatttag tgtaggagag
    16561 acaaataaga ggaatataga tattataaaa gaggctttaa aattggataa aaaagtttat
    16621 tcttttagaa atagagatga gagtaagaag tattatgaca gtttggatag taagatagaa
    16681 catatagata aagtttctca aataatcaat agtgcagata taaataattt attataattt
    16741 atgaaataaa atgtacttat gtgctataga aaaaagagta tagattataa tttttttgaa
    16801 attacaatct atactctttt gttattttgt ttatgtattt atatatttat aaaactttag
    16861 cattttatag ataaccaata gcttcctctc tagttaaaaa aaagtgaatc cctgtagtcg
    16921 attctttcca tctattttca ttaaaatctt cagctacaat cgtttctcct actctatata
    16981 taaaattttc atcagcatat gcacgagcac ttttaaaaga ttctttacca tctatactag
    17041 taattgacag tacttttgcc ttatcacatc tacaagcgtt tgatgtagca gacactcttt
    17101 ttgcgtcgct gggtattaat aattgtacca ttctaaaatt aaaacatttt ttatagccta
    17161 taaatgctcc tgtttcagga caatacaatt taaaaaaacg ggtactttca tctgatatta
    17221 ctccatttaa agtagcatgt tctaaatttg aattttctaa attagctcct ctaatatctg
    17281 agttagataa gtcacaaaat ctaaaacttg caccacttaa gtctgcattt tttaatatag
    17341 aattttttaa tgaacaatcc gaaagataag aattattaaa aatagagttt tctaaatctg
    17401 ctttttctaa atttaaattg ataaggtcag accacgaaaa gtcaatatta cttaagcagt
    17461 atcctcttaa gtccatattt tctaatctta attcttttaa atctgccttt tctccagcat
    17521 tattgttttt aagccataaa taatgtttct caagtgtttt ttctaattct tgtttagaaa
    17581 tagttttcat taaaatcacc ttcataaatt tatttttata tatgtagtag caaaatagtc
    17641 atatatttaa ttatataaca ataaataaaa aacttctaaa taatatataa aatatcatct
    17701 tgaaagtact tttttcaata
//
LOCUS       DAMKMI010000084        17705 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.085, whole genome shotgun sequence.
ACCESSION   DAMKMI010000084 DAMKMI010000000
VERSION     DAMKMI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17705
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.085"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..758)
                     /locus_tag="PC429_002456"
     CDS             complement(<1..758)
                     /locus_tag="PC429_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF3847957.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNV"
     gene            1032..1706
                     /gene="fsa"
                     /locus_tag="PC429_002457"
     CDS             1032..1706
                     /gene="fsa"
                     /locus_tag="PC429_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF3847958.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1828..3222)
                     /locus_tag="PC429_002458"
     CDS             complement(1828..3222)
                     /locus_tag="PC429_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF3847959.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3300..4646)
                     /locus_tag="PC429_002459"
     CDS             complement(3300..4646)
                     /locus_tag="PC429_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3847960.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      4896..4998
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5092..6468
                     /locus_tag="PC429_002460"
     CDS             5092..6468
                     /locus_tag="PC429_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF3847961.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6582..7727)
                     /locus_tag="PC429_002461"
     CDS             complement(6582..7727)
                     /locus_tag="PC429_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3847962.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            7980..8687
                     /locus_tag="PC429_002462"
     CDS             7980..8687
                     /locus_tag="PC429_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3847963.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8734..10329
                     /locus_tag="PC429_002463"
     CDS             8734..10329
                     /locus_tag="PC429_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3847964.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMLIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            10432..11514
                     /locus_tag="PC429_002464"
     CDS             10432..11514
                     /locus_tag="PC429_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF3847965.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            11663..11824
                     /locus_tag="PC429_002465"
     CDS             11663..11824
                     /locus_tag="PC429_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF3847966.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12165..13433
                     /locus_tag="PC429_002466"
     CDS             12165..13433
                     /locus_tag="PC429_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF3847967.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13628..14416
                     /locus_tag="PC429_002467"
     CDS             13628..14416
                     /locus_tag="PC429_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF3847968.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14416..14865
                     /locus_tag="PC429_002468"
     CDS             14416..14865
                     /locus_tag="PC429_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF3847969.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14869..17133
                     /locus_tag="PC429_002469"
     CDS             14869..17133
                     /locus_tag="PC429_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3847970.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVK
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17273..17584
                     /locus_tag="PC429_002470"
     CDS             17273..17584
                     /locus_tag="PC429_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847971.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         6594 a         2078 c         2815 g         6218 t
ORIGIN      
        1 acatttacat taaattctga taaaatatct actatatcag gagtaggcgt tttttctgta
       61 attatagaac taatatcttc taaatgagca aacttgaaat ttccatcaaa tttaaacttt
      121 ttatcttcca taactataaa tactttttta cttgaattta ttatagcctt cttcgtaaga
      181 ccatcttcaa tttcaaaagt tgtaagtgaa gagttgaata catctactcc acaacttccc
      241 ataaaagtct tatcaaactt atatttcgat ataaactcta tagttggagt accgacaaaa
      301 ccgtctaaac tgagatttaa taaacctcca gtggaaatta tatttaaatt atttggtttt
      361 gaagtaacta catttagtat atccagcata tttgtaacaa gtgttacttt tttattactt
      421 tcagctaaca acttcgcaag caagatgtta attgtagaga tatctaaaaa tatagtttct
      481 ctatcttcaa ttaaattaaa tgctttttct gctattattt tctttgttgg aatattaatt
      541 tcttttctgt cttttgtatc ctgattctct agtgtttctc tagctagtac agctcctcca
      601 taaattcttc ttattgaaga aatcttttct atatttttta aatcttttct tatacaatct
      661 tctgtaacat taaatttaga actgagctct ttgacagtaa ctctaccttc tttttttagg
      721 atatttaata tagcttggat tctttcttct gcaaacattt tctacctctt ttcgcaatat
      781 agtattatag taaatattat aagataaaat acaatatatc aactatttta acatgaaata
      841 caaaattaat ttaatataaa aagtatgttt tttctaacta tatatttaga atttagtaaa
      901 taaaaaaatt ataaaactaa gaaggaaaag taaaacaata ataaaaaata aaaaacaata
      961 agcaaaatgt tgaaaataat accaaatcaa tatataataa gaataaagaa agattaaagg
     1021 gggatatttt aatgcaacta atcttagata caggtaatgt ggaggagatt aaggagttat
     1081 gtacttgttt accaatagat ggtgtaacta caaatccaag tattgtttca aaagaaaaaa
     1141 agaattttaa gcaacttata aatgaaattg gtgagattat aggtgaggat atgcctatac
     1201 atgctcaagt gttaagtaca aagtatgaag aaatattaga agaagcatta tatataagta
     1261 gtttgagaaa aaatatatat gtaaaaatac ctgtaacaca agatggttta agagctataa
     1321 aagaccttca tagaaaaggt gtaaaaataa ctgctactgc aatattcact gctcatcaag
     1381 gttttttagc tgcaaaggct ggagcaaatt atattgcacc ttatgtaaat aggcttgata
     1441 atatatctgg tgatggagta gctatggttt cagaacttat aaaaataata gatacttata
     1501 aaatggatac aaaagtactt gcagccagtt ttaaaaatgc acaacaagtt atagaattaa
     1561 tgcaacatgg ggtgcatagt gttacagtac catatgatat ttgtaaatct atgatgaatc
     1621 atccattaac tgattggagt gtagataagt ttatagagga ttgggagaat acttttggaa
     1681 aaggaagtaa aacaaataat atttagtatt atggtaaaag agtagttttg tccaatttta
     1741 caagaactta tataaaaaga tgaaaatatg aattttcttt ttaataattt cgttattatc
     1801 ttaagttaat tcatattttc atctttttta gatttctagt cctataaatt tccaccaagg
     1861 gaatgttata catattatta taaagtaggt taaaggaatc aaagttaaac cgtatttgaa
     1921 aataaccttg ctattataca gattttcacc atgaccaacc attattgtag catgatgaaa
     1981 tggtaatatg tactgtatgc ttactataat gtagacaaat agacataaag taacactatt
     2041 ttcctttagt atatgtaaac ttccaagtaa tggtattact acagatgatg tagtgacagc
     2101 acttccaaga catatattca agattattgt gagagttatc aaaaacaata ttgaaataat
     2161 ttctgaagag tttggtataa actttatcaa ataatttccc attatatcta caactccaga
     2221 gtactttaac actcctccaa ttgaaaatgc tgctataaaa aaaataagga ccttccaatt
     2281 tatttctttt aaagtactaa atccaactat ttttcttagt aataaaagaa gtacacaaat
     2341 tgacattata attaatgtgt ttatgttatg tatgaactga gttaaaaaca taaaaaacat
     2401 tattcctagg cataaaaata cgtgtttttg ttctttgttt agcaaattaa attctttttt
     2461 taaattagaa tatgaaaaat gaagtttatt accctcaaga ttttcattag aaataaattt
     2521 tattgaatgc aattcttttt taaagacaaa ggtaaataaa aaataagtta taaaacaagt
     2581 aattatagtt ggaacagaca tataaaaagc ccattgaccc caatttatat tacaatttcc
     2641 aagttctagt acaacataat ttagaagagt gtctccattt acaaaaaaca ttgatgtaaa
     2701 ggtagatgct gtaaatatac taaataaaag tatagatttt gaatcttcag ttatctgttg
     2761 ttcttttaaa aactccttgt aaaaagcaga tacaagaata acccttggaa atggttgtgg
     2821 gataaaaaat attaaaagta tacctaaaat atacgataaa agaatcattt ttttaggggt
     2881 atttacagta tttaaaatta acttctcaga aataattttt gcgacacctg tttttgtaac
     2941 tgcattagta attatgtatg caagaattat tatgtaaaaa ttttctgtaa ataaaaactg
     3001 taaaataacc tttattggtg caatgttaaa tataaacgct agaattacaa aaaacaatgc
     3061 aacaaaagtt ttttcaactg agtctacagc ccatatcaag atagttaaaa aagataagca
     3121 tatagaaaat tttatgttga aactgtaatt aagagagaag tagagaatta tggtagctat
     3181 aacagtcata taaaattgtt ttggcttaag aataataccc atttttatat tcacctccat
     3241 aatttattat atcataatgg aggtgaatac aatgaatgtg aatacatttt ataaaaagat
     3301 tattcattga ttctatgatt tacaatagct ttagttttcc aatgtccttt cttatagtag
     3361 ataactgtaa caataagacc taaagtccaa ccaaaaggaa tacccatcca aattccgtct
     3421 gttccaaaaa atttagaaag catataagaa gctggaagtc ttccaagcca taaagatact
     3481 attgagctta taagtgcaaa catggaatca ccagcacctt tcattgcact tgaaagtaca
     3541 aaagaagttc caataaataa gaagaatggt cctattataa ataagtaaga agagccaatt
     3601 ttaatgacat ctttatcagt attgaataga gcaattagat tttccctaaa tagcaaaatt
     3661 aaagcaataa ctgtaactgc taaaactaag gtcattttta tagattctcg aatgcctttt
     3721 ttaactcttt cattttctcc agcacctatg ttttgagcaa caaaagtaga aacagcagaa
     3781 ccaagattca taataggtaa gcttataaaa ttttctatcc taagaccagc accaaaagct
     3841 gccatagcag atgtaccata actattaact aaaaattgaa gagccatatt tccaattgaa
     3901 aacaacattt gttgaactcc acttggaagt cctaatctaa gtccatcttt aaatattttg
     3961 ttatcatata caattccctt gattttaaat ttaagcactt catgtttttt atttaaataa
     4021 attatactta ttataaatga tactgcctga gccattatag tggcaagtgc aacgccctca
     4081 acacccattc ttagcactac aataaaagtt aagtctaaaa ctatatttag aattgtggct
     4141 attatcagaa aataaagtgg agttactgaa tctccaagtc ctctaagtat tgcacttact
     4201 gaattatatc cagcagaaaa taatattcca ctaaatatta tagtaagata caattttgat
     4261 tgagcaaaaa cggattctgg tgtattcata agcttaagca ttgggccacc aaatactatt
     4321 ccaagaatac ttattaatat agaaccaata aataaaagta tatatgtagt atcaatagta
     4381 gcttttacct tttttaaatc tcctgagcca taaaattgtg acactaaaat agaaaatcca
     4441 aggctaacac ccataagcgc tgccaccaat aaaaacatta tagggttagc tccagagact
     4501 gccgccattg cttcttttcc aataaatcta ccaactatta tactgtcagc tgtattgtat
     4561 agttgttgaa ataaactacc aataagcata ggcatggcaa aaaagaatat ggactttccc
     4621 tcatgacctg tagttaaatc tttcacctta ttcctccaaa taaaaaaatt attaatttat
     4681 tatagtataa tcttaacata aactttatat ccaaactata aataaattgt atcaaaatat
     4741 catatatttt tttaaaatgt aaagaatata attataattt gaatttaata aatcaatatt
     4801 taatataatc gaatattaat acaaaatcaa tacaatatat acgtatatat tgacaaaatt
     4861 aattatataa attaaaataa tattcgtaaa gaaaaattca tgttttgtac tgtataacat
     4921 ataattttga caatatgggt cataagtttc taccggaata ccgtaaatat tctgactatg
     4981 tatactatta tttagttaat taaatattta attaactgaa ttatgaagga tacggctcag
     5041 cttatcaact actgtgtcgt ttttttgtaa aatttaatag gaggtgttgt tatgcaaaca
     5101 acaaacagta gtactatgca gaaacttttt ccaattttga ctaataaaaa tgtcgacatg
     5161 aagaaggaaa taattgcagg agtaactact tttttaacta tggcttatat aatagctgta
     5221 aatccaaata ttttatctga gacaggcatg cctgcaggtg cacttgttac aggtacttgt
     5281 ttagcagcag catttggatg tattttaatg ggtgtagttg ctaaccttcc ttttgcttta
     5341 gcatcaggta tgggtcttaa tgcatttttt gcatatacag tagttttaca aatgggtgtt
     5401 ccttgggaag tagctcttac agcagtattt gtagaaggta ttatttttat agtattatca
     5461 gtaagtggag ttcgtgaggc tgttgtaaat gcgataccaa aaaatatgaa attagctgtt
     5521 acaggtggta taggaatatt tattgcactt ataggtctag ttaacagtgg aattgttatt
     5581 ggagaccaag ccactcttat caaaatgggt agatttacac cagcagtaat aataacttgt
     5641 gtaggattaa taattattgc tgtattagat aaaaaaagag ttaaaggttc tatacttttt
     5701 ggaatagtag tgagttcatt acttgcttgg gcatttgctt ttataaatcc agagtatgca
     5761 caaaaattag gtatatattt acctggtgga ttatttaaat tcgaaagttt agcaccaata
     5821 gcaggtaaaa tagatttagg atatgtacta catccaacaa atataggtgg ttttttagta
     5881 gtagtttgta catttttatt tgttgatttt tttgatacag taggtacatt agttggtgtt
     5941 tgttctaaag caaacatgtt agatgaaaaa gggaatgttc ctaatgtagg tagagcctta
     6001 cttacagatg cggttgcaac tacagttggt gcaggacttg gagtatctac agttacaact
     6061 tatgttgaga gttcaacagg agttatcgca ggtggaagaa ctggatggac agcaataaca
     6121 gttggaattt tattcttagc tgcaatgttt ttctctcctg tatttatagc aattccatca
     6181 tgtgctacag caccagcttt aatatatgtt ggatatttaa tgcttggtac agttaaggat
     6241 atagagtttg ataatataac tgaaggtgtt ccagcttttg ttactattgc ttgtatggca
     6301 ttaacatata gtataggtga tggtttaact ttaggtatat taacttatgt tttcgtaaat
     6361 atattttata atatatttgg agctaaaaag gcagaagaca aaaaacatgt ttctgtagta
     6421 atgattgttc tggcaatatt atttataata aaactttggc tactataata ggtattattt
     6481 aaaattttaa tttaatataa aataaagggc tgtaatttat tatcaattgt gatgataaac
     6541 tacagctctt ttgtatttgt tgtatttttg tatttattgt attatttaat aatgtctatt
     6601 agttctccat tttgtaaagt tttaggtgta attactatat tgaactcttt ttgcaaatct
     6661 tttataatat ctaattcttt ttttgttatt attgagattg tgttaccact tcgatttcct
     6721 ctagcagttc gaccacatct atgcaggtat tcatttttac tttttggaaa atccaagtta
     6781 aaaacatgac ttacatccac aatatctagt ccacgagcag ataaatcagt tgtaattaaa
     6841 atctttgctt taccaagttt aaatttattt attgcattct ttctatcttc tttttccata
     6901 tttccaaaaa ttcctattgc tttgtaatta tgataattta gttttgatac taggacttct
     6961 atattttttt cattatttac aaatacaata gctttttttg gatttgtagc tgctaatgcc
     7021 tttctaagaa aagtaaattt atcccttatc tcacctaata aatatgagtg attgatgtta
     7081 ggatttattt gtgatttttc ctttgttttt attatttcaa actccttcat aagtttatca
     7141 caaattttta tagtgctatc agtaagactt gcagagcatc ctataatttg tctatctctt
     7201 aacgttgttc ttattatgtc ttctatacaa gttatatttt ttccattcaa tagattgtcc
     7261 acttcatcta agacaatagt ttttatatta tgtgatttta gtttcttttg ttttatcaag
     7321 tctaaaactc ttccacaaga gccaattact atgtgtggtt ttactgcttt tatatttttt
     7381 atttgttttt gtatattgac ctccccaatt aaagctaaag aagtaacgga gagttctgca
     7441 tttttagcca ataattcaac ctggtttgta atctgcatta ctaattcatg cgtaggtgca
     7501 agtataagag cttgtgtttc tctttttgat gtatcaattt tttcaaatat aggtaaaagg
     7561 tacgctagtg tttttcctgt tccagtctgt gaattaatca ataaatcctt attttgaatt
     7621 atattcccta taacaagtga ctgaacttct gttggagaag ttatgtcttg ttttttgagt
     7681 ccatctatta gagtggaact tatttttaat tgttcaaatg tattcatatt ttactcctta
     7741 tattttaata attatagtca actatttttt attatacatt gtttattcaa atcgaactat
     7801 atattctttt ataattatgt gtaatttgaa agtaaatatc aattatattt atacctttat
     7861 atttctgata cctttgcatt ttatagattt ctaaaataaa caaataaaag ttgtaattgt
     7921 ttgatatttg ttatatattg tatataacaa atatcaaaac tataatatgg agggaaatta
     7981 tgttaaaagt aatcaatgta tctaaatcat ataaaaataa aaaagtagta agtagtgtct
     8041 catttgatgt aaatgatgga gagctttttg ggtttatagg acataatgga gcaggtaaaa
     8101 ctactactat taaagcaatt gttggaatac acgattttga agagggagaa gtactaataa
     8161 attctaaatc tataaaaaaa cagcctgttg aatgtaaaaa ggaaatggca tatatacctg
     8221 ataatccaga tttatacgag tcattaactg gtatacaata ccttaatttt atagctgaca
     8281 tatttgaagt tgccaaagat aaaagagaag agttaataaa atattattca aataagtttg
     8341 aactcaataa agcattagga gatttaatat cttcatattc gcatggtatg aaacaaaaat
     8401 tagctataat ttcggctttg atacacagtc caaaaatctt aattcttgat gaaccttttg
     8461 taggtcttga cccaaaagct tcctttatat tgaaggaaat tatgagggag ttttgtgaca
     8521 aaggtggttg tatatttttc tcaacacacg ttttagaagt tgctgaaaaa atttgtgata
     8581 agattgccat aataaaagat ggaaatataa ttgcttatgg aactacagaa gaagttaagg
     8641 gaagtaattc acttgaaagt atctttatgg aattaataga gaaataaaat gttttttata
     8701 caatatagtg tctaaagtga ggaatgataa aatatgagca atctaagtat attaataaaa
     8761 actaatataa taaatgaatt taaattaaat gcgttaaaaa aagctgatgg tagagaaaaa
     8821 caaaaaataa tgggaatgat gctttcagtt gttataatag cgggtctatt aattttttat
     8881 gtaacaagct taagtctctc tttagttgat atcttaaagc aaataaatca actggaaatg
     8941 ctactgatat ttggttttgg attatcaaca ttaacaacta ttgttacatc attatataaa
     9001 acgtcatcat atctatttca agcaaaagat ttagaccttt tgactagttt acctataaaa
     9061 gaatctactg tactagcaag taagatatta atgttggttg gagcaaacta tttattttct
     9121 tcaatatgta tgcttatacc tgcagtggtt tactttttaa attctgatgt aagtataact
     9181 tatttccctt atttgatatt gctattttta gctttaccat tattaccgat agttgcttca
     9241 tcaataattt ttctatttat agggcagata tcatcaagaa taaaatacaa aaatttagta
     9301 ttaatcatag gtagcattat attgatgttg ctttataccc tacttatgtc aagacttaat
     9361 tctatgtcag ttgaaatgct aaagaatagt gtgtctattt cagagacgat tagaaaagta
     9421 tatcctccta tatattattt tgttgatgct cttaaaacag gaagtatagt ttcattttta
     9481 gcatttatag ccatctcaat aataccattt tttatgtttg taactctgtt tgcaaaaaaa
     9541 tttaaagata taaatgctaa gatgagtgaa ggatataagt caaaaaatta tgaatttaag
     9601 ggtcaaaaaa cttcaagacc ttcaaaagct ttattaaaaa aagaatttaa gagatatact
     9661 tcttcgttta tatacttttt aaattcatct gcaggtttgt tatttctaat agtaggtaca
     9721 attttaatat tattatttgg agcagataaa gtgggtgagt tactgaaatt gaatatagat
     9781 tttaatctta taaaaataca attattggga gtattcttct ttataatagt aatgaattgt
     9841 acaacatact gttcaatttc tcttgaagga aaaaacttat ggatactgaa atcttctcca
     9901 atagatgaaa gagaaatatt ctggtcaaaa atattgatga attttttatt gaatgcacca
     9961 ctatctattt tatgtctaat attagtatct ataagattag actttgaaat tcagtttatt
    10021 attgtatcaa tctgtttgat tgtaagtttt tcaatgtttg tagcattgat gggattatta
    10081 tcaaatttat tatacccaaa ccttgattgg aaaaatgagg tggctgtagt aaaaagaagt
    10141 gcaagtatga tagttacaat gttagtatca ttagcttatg taattatact tggaggtata
    10201 tatgtattac ttggaatttc gtttttaaat atatatatta ttttggcaag tatacttact
    10261 ttgatattag attttatgat atggagaatt atttctacta aaggagtaag aatatttaaa
    10321 aatttatagg tgtatttatg tcaaataaag acttatcttt tttagtcttg caaataatag
    10381 gttaagtagt atactatgta tagtgatatg aaaaaattta ggagaaaata aatgagaaaa
    10441 ttatccaaaa gagaaaaaga ttttattcaa tatggatttt tattattttt gatatgtatt
    10501 actacttatt tagtattaac tactttagat ataaaactaa tacctaaaat tataaggcta
    10561 gtaaataaga cttatatatt tcttggcata ttgacaatta tagtatatat gataattgaa
    10621 tctattgtaa ctcatttgat aataaatgca gttcaaaaaa caaaagtaaa atttattggt
    10681 attaaaatgg ctacaatggg attttactat aacttagtta ctccatttgc atctgggagt
    10741 caaccaatgc aaatatatgc acttacaaaa tatgatgtaa gccttagtaa atcagtggct
    10801 atagttacta ataaaactgt agtatttcaa agtacagtta ctgtattttg tggaatttta
    10861 attttgctaa ataagtcatt actgatggag caagtacatt ctgtgaaagt cttgataact
    10921 tttggaatga ttatgaatgt aagtatgttg ttaggtggac tactaatagt atttagtcca
    10981 aaacaagtaa aaatgatagc agaagttttg attaattttt tatctaaatt taaaacattt
    11041 aaatttttag attcaaaacg tgatagaata aatcattata ttgatgatta taattattct
    11101 ataaaaatat ttattaaaaa tgtaagaatc ttagttttaa gtatattact tactgtgtta
    11161 caattaatcg catatttcag tatagtttat tgtgtgtata aagcctcaaa tttaagagga
    11221 gtttcatatg tacatataat gacattacag gtctttttat atatggctat atcaccaatt
    11281 ccaacaccag gaaatgttgg agctaatgag gttacatttt ttactatgtt ctccaatata
    11341 attccaaaag aattattggg gtactctgta ctcttatata gtgggtttgt atactatttt
    11401 atattaatag tttcaggttt atttacattt ggaacgcatt atacattaaa ttcttggaaa
    11461 aaggaaaata aagtaaattc taatttagat aaggttattg aaaaattaaa ttaataaaat
    11521 aattagggaa attctatata taaatatggt gttgaataag ctatattata ttgagatatt
    11581 tccctttttt gattaaaatt atagaaattt gaaataataa gtaataaaca gaaaaagtga
    11641 tattgttttg aaaggagaaa ctatgaaaca agataaacaa aatgaaaaac aaagtgagat
    11701 ttctaagata aaacaaagca atgataataa acttgaaata gctcaagaat tacatgtaag
    11761 taaaaatata gggaatatgc ttggatatag aatagataag aaaaaaatga atagaccaag
    11821 ttaatgaaaa tgttgaaaat ggtacatttt aaaaaatata tataatgaaa tgttttattg
    11881 ttaaatttaa acttatttta atgggggaaa aagtcgaaaa atatctaaaa agatatggat
    11941 ataaaaaata ttaatatata tatactataa acttgacaaa atcaaccttt taataactag
    12001 caaaaaatag caaaattata taagcgttat ttaattgtca aaaatgaaag ctaaattgca
    12061 tgtcctaaaa aaaatgattg ataacattta tttgtgtgct ataattatat agtaaaacaa
    12121 taagtatatt gcataatgta tgcaaatcgg gaggagagac ggacatggaa gcatctatac
    12181 acacaattgc cagcaataat ttattgataa tttttgctat tgtaagtata actggaataa
    12241 tttgtagtaa gcttagtgaa ataataaagg ttccagatgt agtgttatat ttacttgtag
    12301 gtattttaat aggaccatca tttttaaaat ttatagatat tcgtggattt caaatagaaa
    12361 atcaactaat acttacattt ggttctgcat ttattttata tttaggtgga aaagaaataa
    12421 gtttaaaagt attgagaaat gtgaagatat ctgtattctt attagctact ttaggagttg
    12481 tgatatctgc ttttataatg caacaagtaa taggatttac ttttgggata agtgcaatga
    12541 ctgctttact agcaggaact ataatagcat caacagaccc agccactctt gtacctatat
    12601 ttaatcaggt aaagataaag gatagggtaa aacaaactgt aataagtgaa tcagctttta
    12661 atgatgcaac aggagctata ctaacatcag cagtgttggc tgtaattttg tcaaataaat
    12721 tttcattagg tgaaaatatt tatgaactgg ggacaatggt tattgtcgga gttttagttg
    12781 gtttaattac aggaatactg cttttgaaat tagttaatga taaaccttat ggaatattta
    12841 aagattttgc accaataata tctgttattt cagttgtagt tgcatatgaa ttatctacaa
    12901 aattaggtgg aagtggatat atgtcttgct ttattgtagg tataataact ggtaacaaga
    12961 aaaactttaa aatttggtta agtcaaaaat cttatgatgc agatttttat gttgctgaaa
    13021 ctttaggtac tatttgtcgt atggcaatat ttataatatt gggtagccaa gttaatttgg
    13081 ctcagttaag taaatatttt atgccatctg taattacagt attagtatta atgtttatag
    13141 caagaccact ttgcgtttta gtttgtacac ttgtagacag ggaagcaaaa tggagtaaaa
    13201 gtgaaatatt atttatgatg tgggtaaggg aaacaggcgt aataccagca gcactttgtg
    13261 gtattatttc agcaatgaaa gttcctggat acgaagtgat ttcatctgta gtatttatga
    13321 ctatactaat tacattagta atacaaggaa gtactacaaa attagtagct aaaaaacttg
    13381 gattattaga agaagaagtg ataaatataa gtgaaaaagt atcaactttt taaagttttg
    13441 ctttaaaata gtacctgtta atggctattt taatattgtc taatgatgat tattgaaata
    13501 gtcatttttt tatgtataag taatttacag aatttacata ttagacatat ttattatcaa
    13561 aatttgttga agctatgata taattagttg tatttaaaat aagaatagca aataagggag
    13621 gaaatttatg tttacggtta tggatattgt acagccagat acagtagaag aagcgtactc
    13681 aattttaaat aaaagaaaaa ctaaccaagt aattggtgga agtgcttttt taagaatggg
    13741 gaaaaaaaga ataggaacag gaatagaatt atctaaatta aatttagatt atataaaaga
    13801 gtatgaagat tacgttgaga ttggttctat gactactttt agaacattag aaacaagttc
    13861 cataataaaa aataattttg gaagaataat agaagattca gttaaaaata taataggagt
    13921 tcagtttaga aatgtagtaa cagttggtgc aacagtattt tctaaatatg gtttttctga
    13981 cttgatagta gcattacttt cattagatac agaagtagaa ttatataata ttgggagaat
    14041 tagtcttgaa gagtttctaa atagagatta tgagaaagat ttacttatta agatatacat
    14101 aaagaagaca aataagaatg cttcttataa atcacttaga aatgcaaaga gtgattatcc
    14161 aattctaaat gtatctgtat caaaacatat ggaacaattt aagttatgtg taggagcaag
    14221 accgcagaaa gctacgattg caaaacaagc tagtgaattt ctatctaaca atgaaatcaa
    14281 tgaaattaat atagataaag cagtagaaat agcatcagaa gaactaactt ttggttctaa
    14341 catgagagca tctagggagt atagaaaagc tatgagtaaa gtacttttaa aaagagctat
    14401 aatggaggtt atttaatgtt agtaaagatt aatataaatg ggaagaaaaa aagtgttgat
    14461 atagaaccag aagaatatct ggttgatact ttaagaaaag ttggtaattt aagtgtgaaa
    14521 agaggttgtg acacaggctg ttgtggttta tgtacagttt taattgacaa gaaacctaca
    14581 ttatcctgtg caacactgac attgagagct ttagataaag aaataactac aattgaaggt
    14641 ttagaaaatg aagttaaaga atttagtgaa gttttggtaa aagaaggagc tgagcaatgt
    14701 ggtttctgtt ctcctggctt tatacttact gtacttgcaa tgaaggatga gcttgaaaat
    14761 cctacagatg atgaaataaa gcattatctt acaggaaatc tatgtagatg tacagggtat
    14821 atgggacagt taagagctat caaaacttat ctggaggtaa aatagaacat gaatatagtt
    14881 ggaaaaggaa tcaagaaaat agatggaatg agtatcacaa caggaaagcc tgtatataca
    14941 gatgatttat cagcaaaaga tgctttggtg gtaaagatac ttagaagtcc tcacgcttat
    15001 gcaaaaataa aagatataga tacatcaaag gcagaaatgg ttgatggtgt tgaatgtgtt
    15061 ttaacatata aagatgtacc aaaatctcga tttacacttg ctggtcaaac atatccagag
    15121 ccatctccat atgatagatt aattcttgaa gatgttgtaa gatatgtagg tgacgaagta
    15181 gctatagtgg cagctataga tgaaaaaact gcaattaaag caatgaatct tatcaaagta
    15241 aagtatgaag tacttacacc tgttttggat tttgaagagg caataggaaa taaaactatg
    15301 gtacatgatg aagatagaca ttgtaacttt gatataggta tgcaaagaga aaaaaatata
    15361 gtatcccatc atgattatac aaaaggtgat gtagaaaaag tattaagtaa ttgtgatgta
    15421 gtgattgaag aaacttatta tactcaagct caagcacatg caatgatgga gacgtatagg
    15481 gcatacaatt atttagacca tatgggaaga ttggttgtag taagttcaac tcaaatacca
    15541 tttcatgtaa aaagacattt atctcgtgca ctagaaatac cagctagcaa aattagggtt
    15601 ataaaaccta gaattggtgg cggatttggt ggtaaacaaa ctgcatgtgt tgagattttt
    15661 tctgcaattg ttactttaaa aacagggaaa ccagctaaaa taatttatga tagaaaagaa
    15721 acttttaatt gttcaacaag tagacatgca atgagattaa aagttagact aggtgctaca
    15781 aatgatggca taataaaggc aatagatata aaagcacttt ctgatacagg agcatatgga
    15841 gagcatgctt ctacaactat tggtttggtt ggagaaaaaa ctttaccaat gtataataaa
    15901 gttgaagcaa caagatttat tgcagatgtg gtatatacta ataagatgcc agcaggagca
    15961 cttagagggt atggagcatc acaaggatgt tttgctgttg aatcaactat aaatatgctt
    16021 gctaataaat taaaaatgga cccaacagat ttaaggttaa aaaatgtggt aacagaaggt
    16081 gaaactacat ttgcatatgg taaaacttta aatagtgtcg atttaataaa atgtataaat
    16141 aaaggcaaag aacttattgg atggaatgaa aaatatcctt gtaaacatct tgataatgga
    16201 aaaattagaa gtgtgggtat ggcaattact atgcaaggtt ctggtataca aggtcttgat
    16261 aagtcttcag ctgaaataaa attgaatgac tgtggagatt atactcttat gataggttct
    16321 actgatatgg gtacaggaag tgatacgatt ttagctcaaa tggctgctga ggttcttgat
    16381 acaagcattg aaaatataac agttatatca gctgatactg acttagttcc atatgaccca
    16441 ggttcatatg cttcaagtac tacttatgta acaggtatgg cagtagttaa ggcagctcaa
    16501 gacttaagag aaaaaataat ttctgctgga gcaactaaac taggtgttga gaaggatgaa
    16561 gtaaaatttg atggtataaa attaactagt aaaaaaggta gcactttatt attcgatata
    16621 gcagttgatt ctacaattgg tccagatact aatcaattaa taggtcttgc ttctcatggt
    16681 agcccagtat ctcctcctcc ttttatagca ggttttgcag aaactgaaat tgacacagaa
    16741 actggaaaag ttgatttagt aaattatgta gctgtagtag attgtggaac ggttataaat
    16801 aaaaatctag ctaagataca agttgaaggt ggaattgttc aagggattgg aatggcatta
    16861 tttgaagaag ttagatacac agaaaaagga agaatggaca caaatacatt tatgcaatac
    16921 aagattccag ctagatgtga tattggaaat gttaaagtag attttgttga aacttatgaa
    16981 ccaactggac catttggtgc taaatctgtt ggagaagttg ttataaatac acctttacca
    17041 gcaatacaag aagctgtgtt taatggaatt ggtgtaagaa taaatacact ccctattaca
    17101 cctgaaaaag tttttatgga aatgaataaa taattattta tgaaaattta gactagtttt
    17161 acatgttaaa caaccagtta tgtcatacct gttgaaagat atttaataag tagttattat
    17221 aatattatga attttactta taaaatacaa aattaaataa ggggatgatg ttttggctgg
    17281 taatctaaat aatatgagag cagtaaataa ttttagagga gataagaaca ttttagaatg
    17341 tttagtcagc tttgagggtc gttcaataag tcagagaaaa gtaagggtat tttttaaaga
    17401 aaaacaaaat caaatagaaa ttgattttgc agaagaggaa atttctaaat tggttgaaaa
    17461 tgttgtttta aatacatcat atcaagaaat gttatatgat gaaatagaga aacaactgga
    17521 aattgattgt ataggtactt ggatgatatt atctaaatta aaagatggta gtagagttca
    17581 ctgattttaa aataaattat tagagcctaa ttttaaggct cttttttgct gtttttaatt
    17641 aacaatagaa aacttatctt tagtttgtat ataattttaa tatcatatat tctatatata
    17701 taaaa
//
LOCUS       DAMKMI010000085        17319 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMKMI010000085 DAMKMI010000000
VERSION     DAMKMI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.086"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(306..767)
                     /locus_tag="PC429_002471"
     CDS             complement(306..767)
                     /locus_tag="PC429_002471"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF3847972.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(872..2065)
                     /locus_tag="PC429_002472"
     CDS             complement(872..2065)
                     /locus_tag="PC429_002472"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF3847973.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(2088..2753)
                     /locus_tag="PC429_002473"
     CDS             complement(2088..2753)
                     /locus_tag="PC429_002473"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF3847974.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(2787..3899)
                     /gene="ribD"
                     /locus_tag="PC429_002474"
     CDS             complement(2787..3899)
                     /gene="ribD"
                     /locus_tag="PC429_002474"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF3847975.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(4031..4152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4341..6823
                     /gene="recQ"
                     /locus_tag="PC429_002475"
                     /pseudo
     CDS             4341..6823
                     /gene="recQ"
                     /locus_tag="PC429_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     regulatory      6912..7023
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7165..8466
                     /gene="thiC"
                     /locus_tag="PC429_002476"
     CDS             7165..8466
                     /gene="thiC"
                     /locus_tag="PC429_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF3847976.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            8520..8714
                     /gene="thiS"
                     /locus_tag="PC429_002477"
     CDS             8520..8714
                     /gene="thiS"
                     /locus_tag="PC429_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF3847977.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            8707..9510
                     /gene="thiF"
                     /locus_tag="PC429_002478"
     CDS             8707..9510
                     /gene="thiF"
                     /locus_tag="PC429_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF3847978.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            9573..10343
                     /locus_tag="PC429_002479"
     CDS             9573..10343
                     /locus_tag="PC429_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF3847979.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10360..11463
                     /gene="thiH"
                     /locus_tag="PC429_002480"
     CDS             10360..11463
                     /gene="thiH"
                     /locus_tag="PC429_002480"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF3847980.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            11464..12153
                     /locus_tag="PC429_002481"
     CDS             11464..12153
                     /locus_tag="PC429_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF3847981.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12341..13735
                     /gene="yfcC"
                     /locus_tag="PC429_002482"
     CDS             12341..13735
                     /gene="yfcC"
                     /locus_tag="PC429_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF3847982.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            14252..15619
                     /locus_tag="PC429_002483"
     CDS             14252..15619
                     /locus_tag="PC429_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF3847983.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            15644..16252
                     /locus_tag="PC429_002484"
     CDS             15644..16252
                     /locus_tag="PC429_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF3847984.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            16300..16671
                     /locus_tag="PC429_002485"
     CDS             16300..16671
                     /locus_tag="PC429_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF3847985.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            16850..17188
                     /locus_tag="PC429_002486"
     CDS             16850..17188
                     /locus_tag="PC429_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF3847986.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
BASE COUNT         6664 a         1940 c         2896 g         5819 t
ORIGIN      
        1 ataaaataga taattaatat actagtaata tatgctataa ataaaaacaa tagctaaaat
       61 agtaatatat atgtttttat gatattttat gtttagggtt atatttgtaa ttattttaag
      121 cttctaaaag tagatttgat ggataacgta taattttaga aggatatgca atttgtttga
      181 taagaacgta catatgatta aattaaaaat attaagtacc aaaaagaaga aagatagttt
      241 ctccctttgg tacttaatat tttgtatcct ttacactaat ttttaatatt tattaatgat
      301 attatttaat ctagagactt aaataaattt gccatttcta gcgcattcat agcacaatca
      361 taacctttat ttccagcttt tgtacctgct ctttcaatag cttgttcaat attttctgta
      421 gttacgattc caaatataac aggtaattca ctgtctaaag atactttggc tatcccttta
      481 gagacttcac tacatacata atcataatga gaagtagccc ctcttataac acagcctaaa
      541 cagattatag catcatactt accactttta gccatttttt gagcaacaag aggaatctca
      601 aaagctccag gtacccaaac tatttctata ttttctggag aaacgtcatg tcttaaaaga
      661 cagtcttcag ctcctgataa aagttttgat gttataaatt cattaaaacg agaatttatt
      721 atacctattt ttaaatcttt tccaataagt tttccttcat atatcatttt tacttcctcc
      781 aattttattt atgttataga tttatattta taaatatctt taatttaatt ataaaaaact
      841 acttagtaga aaaatatcat catataatca tttatataat atctaaaaga tgtcccattt
      901 tatctttttt agtttttaaa tatagcttat taaagtcatt aatttcttca ttaataggga
      961 ttctattttc gatttttatt ccatatttct ctaattgatt tattttatct gggttattac
     1021 ttaaaagatt tatacttttt atgtttaaat tttttaatat ttgagcagcc atatagaaat
     1081 ctctcatatc ttcttcaaat ccaagcatta aatttgcttc aacagtatcg taaccttctt
     1141 cttgaagttt ataagcctta attttattta aaagaccaat acctctacct tcttgcttca
     1201 tataaattaa aactccactt ccattttcca caattttctt catagaagat tctagctgta
     1261 atccacaatc acattttaga gagtggaaca catctcctgt taaacattca gaatgaagtc
     1321 taactagagt attttctata tttatatttc cataagtaag ggcaatgtgt tcttcatttg
     1381 aataagtatc tctatatcct atgatttcaa aatttccata ttttgtcgga aggaaagcac
     1441 tagatacttt ttctatagta gtttcattta ttttgcggta ttctataagg tcttttatag
     1501 tgatgatttt taaattatgc ttttctttga aaatcattaa atcatcaact cttgccattg
     1561 taccatcatc tttcattatt tcacagataa gaccaatctc tttaaaacct gcaagtctag
     1621 caaggtctac tgaggcttct gtatgtccat ccctgacaag aactccacca gtttttgcta
     1681 ttaaaggaaa gatgtgacca ggcgttttga aatctttact tgtagaattt tcatcacaga
     1741 ataattttac agtttcagct ctttcatatg cagatatacc agttagagta tctgctccat
     1801 ctatagtaac agtaaaggct gtttgaaatg tatctgtatt ttctctaacc ataagaggta
     1861 tattaagact tttaaatttt ttttcagtgg caggtagaca aattagacct ctaccatatg
     1921 tagccataaa atttattgat tcaggagtag ccatttcagc tgccatcaat aaatctcctt
     1981 cattctctct actttcatca tcaactacga ttaccatttt cccatttttt atatcttcaa
     2041 tagcttcttc aatagtatta aacatgtaat tcactccttt attattttta aaaaccattt
     2101 ttaaataaaa aatctttatc tatatttgat ttatggttac tactattaga atttaattcc
     2161 ttgtagttat tagccataaa attattaaca tactttccaa ttacatcgct ttctaaatta
     2221 acgaaatctc caacattctt tgtcaagaga atagtgttaa ttttagtatg aggtataata
     2281 gaaaccttaa atatttcatc atctaaatat gatatagtta aacttacacc atcaatagca
     2341 acagaacctt tgagtatcat tgaggataaa agttttttat caggtcgtat acttactaaa
     2401 actgcatttt catctttttc aaatttgcag atagttcctt taccatcaac atgacctgtt
     2461 actatatgac caccaaatct tgaagaaaga ctcatagctc tttcaagatt taccttgtca
     2521 tttgcattta ataaacctaa atttgtggaa cgaattgtct ccatcataac atcggctgta
     2581 aattcatttt ttccgaggtt tgaaacagtt agacaaactc catttacggc tatactatct
     2641 ccaagatttg tgccatctaa tattttattt gatagtatgg ttacttttcc actcttacca
     2701 ttagcagtaa tttttctaag tattccaact tcttctacta tgccagtaaa catcctaacc
     2761 tcctatatga atgtattaac aaaaatttaa tttaaaacat ctgattctat aagaatatcg
     2821 ccatctatca aagttacatt attgattgtt aactgtgttg aatctgacaa atgatttatt
     2881 ccttctccag aaacgaatgt aggtgcatct ttaccaccta taatcttggg tgatagaaat
     2941 aatttgactt tatcaactaa cttatttcta aagagactgt cattgagagt agcaccacct
     3001 tcaactagaa cagaatcaat gtttaactct cctaatttat acattacaaa ctccaaatct
     3061 actaagttgt tttttgaagg agaaattatt gtttcaacat tattttcttt taacatagat
     3121 aattttatac tttctttgca acatgtgaaa actatagttt tactggtttt atctttaacc
     3181 aaattactat taagtggcaa ctttaggtgt gtatctatta ctattcttgt aggatgagac
     3241 actttttctt gattgagcct acaagttagt aaaggattat cgttcaaaac tgtatttatg
     3301 ccaaccatta tactttggta tttatttctg tatttatgag ttagatacct tgatgattca
     3361 ttagatatcc attttgattc tagtgatttt gtggcaattt taccatctag acttactgct
     3421 gattttacaa tacaaagagg tcttttgttt tttatatgat aaaaaaatgc ctcatttaaa
     3481 tctcttgctt catcttctaa tattcccaca gtaacatcaa tattgttatc ttttaatttt
     3541 tttactccat taccacaaac aagtggattt gggtctaatg tagatattac aactctctta
     3601 atcttatttt gtataatttt atctacacaa ggaggcgttt tcccataatg agaacatggt
     3661 tctaagttta cataaagagt agagtctttt agactttgtt tagcactgtt tatagcattt
     3721 acttcagcat gattagagcc aaatttctca tgatatcctt tgcctattat attactatct
     3781 ttaacaatta cacatccaac taatggattt ggatttacaa agccttctcc atttttagcc
     3841 aattcaatag cttttttcat ataataaata tctttttctt tttgattcac aaaattcaca
     3901 atatgtacac ctcctgttta attggataat ttttaaacaa aaaaccctga gaattaactc
     3961 agggtttaga ataatctaca ataaatgttt aataaatgta gaacaaaatt gctctacaaa
     4021 aacactcatt aaatatcttc ttccatccag actttactgt cggctttgga atttaaccaa
     4081 atcaaccaag ttttacattg gctcgcgggc tataccgccg attgggaatt tcaccctacc
     4141 ccgaagatta agtattaaat ttatatttag aatgtatcat aaattatttt actttgcaaa
     4201 ctattttttt aaaaaatata tgttaatata taatatagaa tgtctataaa ctttgaaata
     4261 ccacgattgt gaatataaaa ataaaaattt gaacataatc aggaaagaag tctaattaat
     4321 ttaaattagg agagtaatat atgaataaaa aacttttaga aatactatca aaatactatg
     4381 gatatacaag ttttagaaaa ggtcaggaat ctataataaa ttctatctta agcgaaaaag
     4441 atgtgctagc tattatgcca acaggagggg gaaaatcaat atgttatcaa attccagctt
     4501 tgattttaga tggaatgaca atagtaattt ctccacttat atcacttatg aaagaccaag
     4561 tagatgcctt aaagactatg ggaattagtg ctgcttttat aaatagttca ttaagtagca
     4621 aagaatttaa tgaaatatta aataatatta gaaaaaataa ttataaaata ctctatattg
     4681 ctccagagag attagatgca caagagttta ttgagctaat aaataataac aatgtaagtc
     4741 aagttgcgat agatgaagca cattgtgtat ctcaatgggg gcatgatttt aggttgagtt
     4801 atagaaggat ttctgatttt ataaaaaatt tgcctaaaag acctatagtt acagcattta
     4861 ctgcaacagc ttcagaagag gtaagaactg atataataaa tttactgtgt ttagaaaatc
     4921 cagattatta tataactgga tttgatagag agaatttatc aataaatata gtaaaatcat
     4981 ctagtaaaaa taagtatatt ttagattata ttcaaaatca taaaaatgag tctggaatta
     5041 tatatgttgc aactagaaag gaagttgaaa atatatataa tggtttatct aagagaaata
     5101 tatctgtatc aaaatatcat gcaggtttat ctaaaaatga aagaagtaaa aatcaagaag
     5161 attttataaa tgataatgtg gatataatgg tagcaacaaa tgcatttgga atgggtattg
     5221 ataaacctaa tataagatgg gttatacatt acaatatgcc tcaaagtata gaaaactact
     5281 atcaagaaat aggtagagct ggtagggatg gagaaaaaag tgagtgtgta cttttatttt
     5341 ctccaggaga tgtacatata caaaaatatt taattgatat tggagttgaa aatcctgaaa
     5401 gaaaattatt tcagcataag aagcttcaat acatgattga cttagtttac agtaattcat
     5461 gctatagaaa gacaatattg agttattttg gagaaaatta tatagacaat tgcaataatt
     5521 gtagcaattg tttagtagag ggtgaaatag tagataaaac tatagatgca caaaaagtca
     5581 tttcatgtgt agctagaatg aaaagaagtt atggtgttac tactattgta gatgtgctta
     5641 gaggttctaa gaataaaaag attttacagc ttggattaaa tactttgtct acatataaca
     5701 ttatgagaga ttattctagt gaagacttaa agaattttgt aaatacattg gtttctcatg
     5761 gttttttaga tttagttgag actctaggca atggaaatag aggtagtttt cctactataa
     5821 gattaaatga gatgtctatg aaagttttaa aaggtgaagt gaaagtagag tttaaagaaa
     5881 ttgttatgac aaaatcattg gaagtagagg atgagttata taatgcttta agagaactta
     5941 gacattcaat tgcaagtgaa gaaaggatag ctccatatat ggtatttgga gatggtacac
     6001 ttagagccat gtctagtagt tatccaatta ataaagaaga aatgctagat atctctggag
     6061 taggagaaat taaataccaa aaatatggta gagattttga agcagtaatt aaagaatatg
     6121 tcgaaaaaat aatataaata aaaaaataga tgaaaaaaat gtcgattcaa agaacgttaa
     6181 tagtgatttc ttagaggtta atacagataa agttttatat gaaaaactgt tagtcattag
     6241 agaagaatat gcaacaaagg aaaaattacc tgcatatatg attttagcca ataaggcttt
     6301 gaaagaaata agtggaagat atccacttga tgaagaacaa ttaaaagata tttctggcat
     6361 aggtggagta aaatttgaaa aatatggagc tagtattttg gcagaggtta ataaatatgt
     6421 aaaagaaaat aatattaatg ttatttggga gaaaaagggc aaaaggaagt taatattaga
     6481 tggagaaagt agaaaaaata atgaaatagc attagatttg ctaaatcaag gaaaagatat
     6541 acaaagtatc agtcaagaaa tagaagtttc actgtcaacc attattggat atgtgcatga
     6601 ctatgtaaag gatggacata gtatatcatt tgaattagga ctagaaaatt tttataatga
     6661 agataaaaag aagatgattt tagaagtttg cgaaaaagtt ggctatgata atttaaataa
     6721 cataaaaaga aaacttccag attcaataaa gtatgaaaat ataagagctg tgatacttga
     6781 ctcttatctt gaaaacttaa aaaataatat tatagcagag taatttttaa cttaataaaa
     6841 aataaaattt gtattattgt gttgaactaa taattttgct gtgttatact gaatttgtaa
     6901 aaataatgaa aaattagagc taggggcgct agaattggct agctgagaga taaagccgta
     6961 acttacaaac cctaataacc tgatcaagat aatgcttgcg aagggaagct ttttaatata
     7021 attttattat ataaataggc aaattattca atgttaggag aaatttgtct aagtgtatat
     7081 acgattataa gagtgctttc caaacgagga aaagcacttt ttttattttc aaaaactata
     7141 actttaatct tggaggattg taatatgaac tatacaacac aaatggatgc tgctagaaaa
     7201 ggcataataa ctaaagaaat ggaaatcgtt tctcaaaaag aacaggtaga tgtaaatgaa
     7261 ttaagagaat taatagcaaa tggacaagtt gttataccag ctaataaaaa tcataaatct
     7321 ttaagtgcag aaggtgtagg taaaaattta agaacaaaaa taaatgtaaa tctaggaatt
     7381 tcaagagatt gtaaggatat agaaaaagaa cttgaaaaag ttagagtagc aatagacatg
     7441 aaagctgaag ctataatgga tttaagtaat tatggcaaaa ctagagaatt tagagaaaag
     7501 gttgttgaaa tgtcacctgc aatgataggt tcagtaccta tgtatgatgc agtaggatat
     7561 ttggaaaaag aattaaaaga tataacagaa gaagagtttt taaatgtaat aagacaacat
     7621 gctattgatg gagtagactt tattactata catgcaggtc ttacaagaag tgtttgccaa
     7681 aaaataaaaa atcatgagag attgactcat atagtatctc gtggaggttc tttattgttt
     7741 gcatggatgg aattaaataa taaggaaaat cctatttata ctaattttga taaaatattg
     7801 gatatatgtg aagaatatga tgttacattg agtttaggag atgcttgtag accaggttgt
     7861 ataaaagatt ctactgatgg tgttcaaatt caagaacttg tagtattagg tgaacttact
     7921 aaaagagcat gggaaagaaa tgtacaagta atgatagaag gtcctggaca catggctatt
     7981 gatgaaatag aagcaaatgt agtattagaa aagagattat gccatggagc acctttttat
     8041 gttcttggac cattggttac agatatagca ccaggttatg accatataac aagtgcaata
     8101 ggaggagctt tagcatgtgc taaaggagta gattttttat gttatgtaac tccagcagaa
     8161 catttaagat tgcctaattt agatgatatg aaagagggta taatagctgc aaagatagct
     8221 gctcatgcag gagatatagc taaaaatgtt aaaggtgctc gtgaatggga taataagatg
     8281 agtaaagcta gagctgattt agactggtgt gagatgttta gacttgcaat agaccctgaa
     8341 aaagctaaga gatacagaga tgaatcaact cctactcatg aggatagttg tactatgtgt
     8401 ggaaaaatgt gctcaatgag aacagttaag aaaatattaa acaatgagga acttaatcta
     8461 atataatttt tggctagtat gactggaata aaaataagct gaaaagagga ggtgatttag
     8521 tgaaggttaa tgggaaggaa attgaatttg aaaaagattt gactgttata gatttattaa
     8581 ataaatataa tttaaaaagt gatagagttg tagttgaagt gaatttagag ataattgaag
     8641 aatctaacta caatacatat gtattaaaag acgaggacat tgtagagctt attagcttta
     8701 ttggaggtgg ttaaaataaa aatatttgta aatgaattat taacagatgt tgaggaaaat
     8761 acaactgctt ataaagttag agatagctat aacaataaat gtgatgtatt ggtgttaaat
     8821 ggatatccaa ttaaaacaga tatgaccctt tgtgaaaatg ataaattaac acttatacaa
     8881 aaaggagtta agccatcact tgatgaattg gaaagcttgt tgattgctag acatactcca
     8941 aatatacata ataaattaaa aaaagggaaa gttgctatat tgggattggg gggacttggt
     9001 tccaatatag ctatctcttt agctagaata ggagtgggag aacttttact tatagattat
     9061 gatgttgtag aaccatctaa tttaaataga cagcagtatt ttatagatga tataggaaaa
     9121 ctaaaaactg atgccatgat agaaaatata aaaaaaataa atccatttat aaaattaaat
     9181 aaacgagata tttttttaaa caagaacaat atggatacta ttaaggattc agatttaata
     9241 attgaggcct ttgatgatgc ttcatgtaaa gcacaggtat gtaattatgt attaataaat
     9301 ttaaaagata aatatttaat agcatcatct ggtatggcag gttactatga ttcaaatata
     9361 ataactacta agaagataaa agacaggttt tatatatgtg gggattttgt gaatgaagct
     9421 aaatttggtg aagggcttat ggctccaagg gttgccatct gcgcaaatca tatggcaaat
     9481 ttggcaacgc aaatacttat agatatgtaa atattaaaat tagatataaa aaagattgaa
     9541 tattaaaaaa atttaacgtg gagggaaata atatggataa attggtactt ggtggacatg
     9601 aatttaatag cagattattg gttggaacag gtaaatatgg ttcaaataat atattgcctg
     9661 aggtaataaa agaaagtgga agtgaaataa taacaatggc acttagaaga gtggatttag
     9721 acaataaaca agaaaacata ttaacgtata ttccaaaaga gatgacaata cttccaaata
     9781 catcaggagc tactaatgca gaagaagctg taagaatagc tagaatatct agaaaaatgg
     9841 gatgtggaga ttttataaaa atagaagtaa tatcagatac tagatattta cttcctgata
     9901 atgaagaaac tataaaagct acaaagatac ttgctgatga ggggtttata gttcttccat
     9961 atatgacacc agacctttat gctggaagaa gacttataga agctaatgca gcggcagtta
    10021 tgccacttgg agctccaatt ggttcaaata gaggtcttca aatgaaagaa atgataagaa
    10081 taatgataga tgagttggat ataccaataa tagtagatgc aggtattgga aaaccatctc
    10141 aagctatgga ggctatggaa atgggagctg atgcagtact tgtcaacact gctatagctt
    10201 cagcaggaga ccctgtacaa atggctagag catttaaatt agctgtagaa ggtggaagag
    10261 aagcatatat tgcaaaaaca ggaaatgtgt cagaatttgc aaatgcatct tctcctttaa
    10321 caggtttttt aggcaattta tagaaagtgg tgattggtga tgagttttta tgatgtaata
    10381 gaaaaatata gggattttga ttttgatgga tatttaaata atgttactga taatgatgtt
    10441 ttaagaagtt tatcaaaaga caagcttgaa gattttgata tattaaattt gctttctaaa
    10501 actgcagtaa aacatttaga agatatggca caaaaagcac ataaattaag tgttcaatac
    10561 tttggtaaaa cagtatgttt atatacacca atgtatatag caaattattg tgttaatcaa
    10621 tgtgtttatt gtagctataa tataaagagt ggaataaaaa gaaaaaaatt aactatggat
    10681 gaaattagag aagagggaga agcaatttca aaagaaggtt tcaaacatct acttgttttg
    10741 actggagaaa gtagttttca ctcaagtgta gaatatatag gtgaggctat agaaatatta
    10801 agagagaaat ttccctctat aggtatagaa gtttatccaa tggaagtaga agaatataag
    10861 tacattgtag ataaaggtgt tgaaggatta acagtatatc aagaaactta tgacgaggaa
    10921 atatataaga gagtacatat aaaaggccca aaatcaaatt ataaatttag actagatgct
    10981 cctgaaagag gggcaaaagc aggtatgagg actttatcaa taggtgcact tttagggcta
    11041 aatgatttta gaaaagaaac tttttttaca atattgcatg ggaaatactt aaaaacgaaa
    11101 tatcctcata ttgagctttc atattcaaca ccaagaatga gaccttttaa agggtgtttt
    11161 gaggaattgg tagatataag tgatactgat ttagttcaag ctatggtatg tatgagatta
    11221 tttgaccctc atgcagctat aaatatatcc actagagaaa atttagaaat gagaagtcat
    11281 ataatacctt taggagtaac taaattgagt gcaggtgtat ctactgatgt aggaggtcat
    11341 tctcaagatg aacatgatac tgctcaattt aagataaatg atgaaagtac tgttaaagat
    11401 gtggagaaaa tgctaaactc aattggttat cagcatgtgt ttaaagattg ggaaagattt
    11461 taaatgtatc tgataacaaa cagaaaatta tgtagtgagg aacgatattt agaagttatt
    11521 aaagagtcta tattgagtgg tgtagaaaat ataataataa gagaaaagga tttagaatat
    11581 caagagctaa gaaaattata tatgaaaata aaaactaaaa tcaattgtat agattttcaa
    11641 gagcaaatat cagatgaaag tttgaaaact aatataaatc aaaaagagtg tagaaataaa
    11701 ttcaaggtta attttataat aaatagtaac attgaatttt ttgaaaaagt ggattgtcaa
    11761 ggtatccatc ttccttttaa attattcttg aatttaattg aaaataagta taattttaat
    11821 gaaaataaaa tactaggatt atctttacat aaagtagagg aagtagatta tttagaaaaa
    11881 ttaataagaa atcaaaatat aaaaatagat tacataacat tatcacatat atatgaaact
    11941 aaatgtaaag aaggattaaa tccaaaagga attgagcttt taaaagaagc taaaaagatt
    12001 actgatatta aaataattgc acttggggga attttaccat ctaatgtcaa ggaaacatta
    12061 aaatactgtg atgattttgc tataatgtca actataatga ggtctaaaga tattaaaaaa
    12121 accatatcaa attataatga aaagttgaat tagtaaatat agtatattga atttttaact
    12181 taaataaaaa taaaaaagtt atcttttaaa ttttaaaagg tagctttttt attttatatc
    12241 tttgctaata aaaaattgta aaaagggaaa actttagtca gagagattaa acaatatcct
    12301 agaaagattt aaaatttagt aaagtatgga ggacattata atgactgaca gtaaaaagaa
    12361 acgatttatt ataccacaca cttttacaat aatattttta ctaatagtct tgatggcaat
    12421 attgacttgg atagtaccaa gtggtgaatt tagtagagaa gatgtagaag gtagaatggt
    12481 agttgtacca gggacttatc aaagtgtaga aagtaatcct caaggatttg cagatgtttt
    12541 taaagcacca atagaaggct ttatcgattc agctgaagtt gtaggttttg ttttaatagt
    12601 tggaggtgct tttggtattg taaataggac tggagctata gaagcaggaa tagcttcggt
    12661 tatctctaag tttaagggaa aagaacttct tataattcca atatcaatga ttttatttgg
    12721 acttggaggt acaacatttg ggatggcaga agagacatta ccattttata tgatatttgt
    12781 tccactcatg acatccttgg gatatgattc tctaacagca atagcaattg tatttatagg
    12841 agcagcagca ggagccgccg cttcaacaat aaatccattt tcagttggta ttgcacaggc
    12901 actagctcaa cttgctcctg gttcaggagt aacttacaga gtagttattt ttatagcata
    12961 tatactaata agtatagcat ttgttatgta ctatgctaag aaggtaaagg aaaatcctga
    13021 aagttcaata gtatatgaaa tagataaaaa taataaatca atacttaatc aaacttcaac
    13081 agaaataaaa gaatttactt ttaaagaagt atctgttctt gctatatttg gaatagggat
    13141 ggcaataatg atttggggtg ttttaagtca aggatggtac attccagaaa tatcaatgtg
    13201 ttttgctgca ataggtgtgc tttctggatt ggttggtaga ttatctcaaa ttgaaatctc
    13261 agaaagtttt atagatggag caaaagattt agcatctgca gctcttgcta tagctttggc
    13321 tcgtggaatc gtaataattg ctcaaaatgg atttataata gatactatat taaattccgc
    13381 agcagccttt ctaggtgggc ttccaaaggt tatatttgta tatttatcat ttttcttgga
    13441 agctgcattg gcatttttaa ttccatcatc ttcaggtctt gcttcactta cagtacctgt
    13501 acttgctcct cttggtgatt tggttaatat atcggctcaa gtgatagtaa ccgcttatca
    13561 atttggtgtg ggtcttacaa actttataac tccaacttca ggagtactga tgggagcact
    13621 tgcagtagcc catattccat tttcaaaatg ggtaagattt ataatgccta tcttgttgat
    13681 tttgacagct gtatctctag tctttttaac aataggtatt tatatagggt tttaattaag
    13741 tgtagtttta tttaagatat ttagtaatgg tataaaatga taatatgttt tcaataaatt
    13801 gccactaatt taattagtgg taatttgttt tttgtattta aaaataaata tatttacttg
    13861 tattttgaat taatatctac ttagacattg ttttaattgt tatattttca tttaattaag
    13921 cattgattta tcatttttta tatttcatat aacaatgcaa aaaataattt ttatatagta
    13981 aatgacaaaa gtttacaata aatgtatatt ttcttactca ttccttaaaa ttactcattt
    14041 agtttaataa ctctcatttc atatgtaaag ttggtaaaaa aattaaaatt aaaagtaaaa
    14101 aactaaataa attaatgtta atttgaaata tatatgaagg tttttggaga atacttactt
    14161 atgataggaa aaaatgttaa aataatagtg taatcaaata taaaggactg aaataattaa
    14221 cagttattaa atattaggag tgtaaaaatt tatgggatta aaagaatttg attttattga
    14281 agagtctatt gatatgttga ggacaatgtc accaacatta gaaactatat cagatgaaat
    14341 agaagagtat tttgaaaata tcttggatga aaagaatcaa gaatatataa atgtaacctc
    14401 tagaataaaa tcagaatcaa gtttaagaga aaagataatt agaaatagat atttaaaaaa
    14461 atatggagaa gcaagtaatc ttattcataa tgtttctgac ttaataggcc taagaattga
    14521 gtgtagattt attgaggatg aaaacaaaat atatagactt ttgagaagat attttaataa
    14581 aacagatgac aaaataaact attataataa agagaataag aatattaaat taaaactatc
    14641 tgagagacaa cccaataagc aaaaaaatgg atttgaaata tataaaatag atggagtttt
    14701 tgcgtattta gatagagaag taaaatttga acttcaaatt aaatctcttg taaatgtgtt
    14761 ttggagtgaa atagaacata aaataattta taaaaacaat acatatttac ttgctgataa
    14821 atttataaag gatatgatgg actctataaa gaataatctt actatgatag ataatcagct
    14881 tttaagtatt tataaaaact ttcattcagg gaagtctttt aatatgaaag taagtaaaaa
    14941 agaaattgaa aaattatttg cgaaactagt ctatgacgct ttttctgaaa agatgaataa
    15001 aagtataggt tttgttgtag attttaaaaa accatgtgag actatattga actattcttt
    15061 taataaacaa gatataagtg atgaagtttt aggtaatttt atgcttgatg agtttgcaag
    15121 attaaatgaa attgtaaaca aggatataga ttttaatgag caaatagaat ttgaaagaga
    15181 accaacattt gatgataagt tttgtaaaga cctaggaaat cattttagaa gtagattaaa
    15241 cacagaattt ccatggaacc tattttttag aatattattt gaaatagagc catataacaa
    15301 taccaaagac tttgaaaact ttgtagagtt tataaaagaa aatgtgctac tagaagaaaa
    15361 tagggagcaa ttattatgcc aatttgaaga agatagcaaa cttataattg aagatatata
    15421 tgattgtata tttaatagta taagtgagat tgatagtgtt gaaattttat ataactataa
    15481 tcttgaaaaa ataaacttta catcaagtga aatcataaat tgtatatgta gagaatatga
    15541 gtgttatggt gaatacttag aagaaaaaga aaagcttatg aatacattta aagaaaagat
    15601 tattcaaatc tttgagtaat aggtcaatgc tgaagtggcg ctaatgcgcc atttttcaaa
    15661 tatatatgaa ttaagtaaaa ttattttgga ggaggcgatt tgtatagaga aaacaggtgt
    15721 aattttggca gggggaagaa attctagaat gggtagagat aaagcctttt tagagttaca
    15781 tgataagctt tttattgaaa ttgcaataga agcatttaaa aactttgatg agttgataat
    15841 aatatctaat aatgaagaat tatattctaa atacgatatt aaagtttata atgatatagt
    15901 aaaagatgtt ggtccaattg gaggaatata tactgctctt gaatatgcaa agtatgatat
    15961 agttacaatt gcatgtgata tgccatatct aaataatcaa atagtcgaaa gaatagctaa
    16021 taaaatgaat gataaaagtg tgatttcagt gacaaatgga aagttacaac cattgtgttc
    16081 tggatataaa aagagtataa taaataaagt atctttatgt ataagggaaa atgatttaaa
    16141 gctcagaagc tttatagata aaatagataa aagttatata tactttgatg aagaagattt
    16201 atttttaaat gtaaacactg ttgatgagta tgaaaaatta actaaagttt aattagctat
    16261 ggttgtaaaa tagagtagga tttatatgga ggagagttta tgaatttact taaatgcgat
    16321 tcttgggcag taatattaaa taatagcgat aaagaaagta aagcatataa aatattagat
    16381 gaacttaaaa ggaatatgta caaagttgtt gctatagatg aagagaaaaa acctattgaa
    16441 ggaatagatg tgtatgaatg tttaaaagat gttccacaca atattgatgt tgtagctatt
    16501 attgataaac aatctaagat ggatgtgatt ttagaagaag tagagctttt ggatatacaa
    16561 aatatgtggt ttgaaaaagg aagctttagt gaaatgatga ttaaaaagac taaagattta
    16621 aaattaaata tagaatataa tttaagttta tatgatgagt tgactagata aaattatata
    16681 tcttaataat aaaaatataa ttagttaata aaacatatta ttttatagta tagattggtt
    16741 aataaaagta cagattggtt aatgaaacat taaattacaa tatttgttaa aaaaattgtg
    16801 agttacatga tataatgtat taaataggat actaaggagt tgatatatta tgttaatagt
    16861 aactacagaa aaagtagaag gtaaaaagat atcaaaggtt ttaggattag tgagaggaag
    16921 tacaataaga gcaaaacatg ttggaaaaga tataggagca agttttaaga atcttgttgg
    16981 aggagaactt actggatata atgaaatgct cactgaagca agacaaattg ccataggtag
    17041 aatggttgaa gatgcagaag ctaaaggtgc aaacgcagta atagcattta gactgtcttc
    17101 agcttcagtt atgcaagggg cagcagaaat gcttgcttat ggaacagcag ttgttttaga
    17161 agatgataat agtattcttg aaaaataaaa tataaatagt taaaaagagg catcttttaa
    17221 aaattttatg gatgcctttt tttatttgaa aaaactaatt tgagaagaaa ttttaactat
    17281 tatgacaata aaattagggt gatattatat aaataagta
//
LOCUS       DAMKMI010000086        17293 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMKMI010000086 DAMKMI010000000
VERSION     DAMKMI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.087"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            38..784
                     /locus_tag="PC429_002487"
     CDS             38..784
                     /locus_tag="PC429_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF3847987.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKLYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            845..1519
                     /locus_tag="PC429_002488"
     CDS             845..1519
                     /locus_tag="PC429_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF3847988.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1888..3234
                     /locus_tag="PC429_002489"
     CDS             1888..3234
                     /locus_tag="PC429_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF3847989.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQNIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGGNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIITLSGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYCGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGENTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3261..4661
                     /locus_tag="PC429_002490"
     CDS             3261..4661
                     /locus_tag="PC429_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3847990.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKSICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAIVCEDKQ
                     GESGHYKNLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNKDNWEDFKEDKH
                     KVYLVEALETKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSSINGIGIS
                     DEDYGDKVKIKKGNIYINSKDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4871..6307
                     /locus_tag="PC429_002491"
     CDS             4871..6307
                     /locus_tag="PC429_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF3847991.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPTKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSNPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIETSYGGKW
                     EKLGSEVIGEETVYYYKHHEPDKTNQIQ"
     gene            6651..7595
                     /locus_tag="PC429_002492"
     CDS             6651..7595
                     /locus_tag="PC429_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF3847992.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDKNKQISIFD"
     gene            7764..8696
                     /locus_tag="PC429_002493"
     CDS             7764..8696
                     /locus_tag="PC429_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF3847993.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENTLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8801..9268
                     /locus_tag="PC429_002494"
     CDS             8801..9268
                     /locus_tag="PC429_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF3847994.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9512..10366)
                     /locus_tag="PC429_002495"
     CDS             complement(9512..10366)
                     /locus_tag="PC429_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF3847995.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10532..12496
                     /locus_tag="PC429_002496"
     CDS             10532..12496
                     /locus_tag="PC429_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3847996.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12496..12957
                     /locus_tag="PC429_002497"
     CDS             12496..12957
                     /locus_tag="PC429_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF3847997.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            12960..13280
                     /locus_tag="PC429_002498"
     CDS             12960..13280
                     /locus_tag="PC429_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF3847998.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13296..14396
                     /locus_tag="PC429_002499"
     CDS             13296..14396
                     /locus_tag="PC429_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF3847999.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14516..17116
                     /locus_tag="PC429_002500"
     CDS             14516..17116
                     /locus_tag="PC429_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF3848000.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INVQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDENIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRKNGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYHIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6870 a         1779 c         2996 g         5648 t
ORIGIN      
        1 ttatgaaaaa tgtaaactgt gaaatggagg tagtaacatg aaaattgaaa atctttctat
       61 tgatgatatt aaacgaggct atatatttaa tgatgattta aattgttata gttgcattaa
      121 atgtacaaaa acatatgagg taggagaaat atataaaata ggtaatagat tctttgaagc
      181 ttcaaaagct attgaacttc atatggaatt agaacatagt gattatttag agcaattgct
      241 taatagtgat tcaaaataca acacattgac tgaaaatcaa aaacaattat ttcaattatt
      301 tattagtgat ttaccagata aggagatagc aaaacaggtt ggagtatcca catctactat
      361 acgccatcaa aaatttattt tcagagaaaa agcaaaacag gctaagctct atttagcact
      421 atatgaatct gtatttgaag ataaatctaa taaaagtagt actataatac caatccatga
      481 acatgctcga atggtagatg aaagatatgt agtaactgaa gaagagagaa aacacatttt
      541 ggatacatca tttgaaagta tgaatccttt aaaattaaaa gtattttctt caaaagaaaa
      601 gaaaaaggtc gtaattttat ctaagatttt ggaacaattt gaacgtggga agaaatattc
      661 agaaaaggaa gttaatcaga ttttagaatc tatatatgat gattttgcaa caattagaag
      721 atatctaatt gagtatggtt ttatgagtag aaataaagaa tgtacaaaat attggttaac
      781 ataattaaaa taataaattt tactaatgtg taatgttatt tttatctagg gggaggagtc
      841 aaatatgaat gatgaattaa aatctatttt atacaataaa ggtgtagaca ttgttcgctt
      901 tgttgatata tctgaatttc caataaatca aacacaaggt ttttctaaag caattttatt
      961 ttgtataggg ttatcaaaaa aatttattac agatatatac aataacttac ctacagatag
     1021 tgatgagttt ttggaaaaag aggagaaagt agaagaatta gctgactgga tttcaaaata
     1081 tattcaaaat aaaggatata gagcatattc tcaatcggag aaaaacaatc tagaacatgg
     1141 atattttgaa aaagcctata ttaatccaga aatgcaatca gggattagtc ctttaccaca
     1201 taagacaata gcaaatatta gtggcattgg atttatggga aaaaataatt tgtttgtaac
     1261 agaggaatat ggatgtgctt ttagcatgtg tacagtactt acagatgctc caatttctgt
     1321 tgaaagatat cctttgattg attcaaaatg tatggattgc aatgtttgtg tagagaactg
     1381 tcctgcaaaa gctatacatg gaaatgaatg gactttacct ggaaaaagag aatctattat
     1441 tgatgtttca aagtgttttt gtgtattgag gtgtatgatg agttgcccat ggtcattaag
     1501 atatgcaaat caaaaataac tttactagag aaggtattaa attgaatatt ataaaatttt
     1561 taaaatgtaa gattatataa gatttctcaa aataaaaatt tgtatagaac actcagaaat
     1621 gagtgttttt ttattgggat taaattatat tgatacaaag cttaactatt ttaatcatta
     1681 tataaaattt gtattaaaaa attaagaata acttggtaat agtgaaaata taatgagatt
     1741 ataattgatt tataatataa ctataatgat attgtaaaaa tgaaaattgt cctctcaaaa
     1801 ttcccaaaga gagtaaaagt cacttcagtt atagtcaaaa aaatttttct gtgaaaagaa
     1861 tttagatttg aaaaggagga attaatcatg gaaattgaaa ctaaaactcc agattggatt
     1921 aatgtactag aattaaacag taaaatagat attactggga aatatgatgt aagtaatatg
     1981 atacaaaata ttgtctctga taagtcacta gaaggttcca ttatatattt tccaaaaggt
     2041 aattacttat ttgagaaagg tattaaaatt agtcaacaaa taacacttca aggagattct
     2101 tattatggag gaggtaatca agtatcaaat ttagataaaa aacaatcaat cataggaaca
     2161 accaatttta taacccgagg agtttctaat atgagtatta ttacattgtc aggaacaagt
     2221 caatgtatca aaaatattaa tttttattat gatagtcatg atatagagaa aataccgcct
     2281 aaaaatgtat ctgctattac agaatatgga gaaacacaag gactatctca ttttgaacat
     2341 ctgtttattt ctggattttc agggatagga attgaaattc catactattc aactggtaat
     2401 gatattactg ttagttcatg tggtcttggt atgaggcttg gagagaagtc aatgctttct
     2461 agtagcaaga tttacgaatg taaaaatgga atggagatta ctactggtgt tagcttaaat
     2521 aatgtgagaa tagaagaaat aaaagaaata ggtattaata ataaaggatt tggttttaac
     2581 ctgattatga atcttatggt cgaccaatgt ggatattgtg gatttatgtt tgaaaaaatg
     2641 tgctatagcc agataactgc cagaattact agatgcggtc agtattataa aagtgttgat
     2701 tataatgctt atgagaatat ggaagatagg aaagaagaag cttattccat tttttatgga
     2761 gatattttag aaaattgcaa tattgtattg atgaatagta atattgatac cttggaaaat
     2821 gattcacttg atgaacataa gatatatgta atcaaggcaa atgaaacaaa caatgtaatg
     2881 ctaacatgtt atgctgaagc agataacttt atacaatgtg caaagggcaa cttgctgtta
     2941 gaaaatggtg aaaatactta taaattttat aatggtgaga tttgttctgt aggaggtgtt
     3001 caaatttctg atatagatga aaaagattta atcaaaataa tagagaacac tttgtatata
     3061 gatgataaag gtaagtattt aatggttcct agattaaaaa agaatactgt aatatattca
     3121 gtactagatt ctacagaaga aataaataaa ttacatggtg gaatatggga acagattgat
     3181 gtaaaagtat tagctgggga atccatgtac tattataaaa agataaaaga ttaaattatc
     3241 aaactaggag gtagtgatta atggaagagt ctataataat taaagatata agtggctgga
     3301 ttaatgtcct tgattatgga atttgtaatg atgaaacaaa agatgtaagt tttaagttgc
     3361 aagagattat ggatgctgta ccagaagatt ctattatata ttttccaaaa ggaaaatact
     3421 tatttgaaag tggtgttaag attaagaaac gattaacttt atgtggagat tcttatattt
     3481 tggcgaatgt tcctaaaaat actgctggag taacacaatt tacttttaaa ggaaagaaaa
     3541 atcctaaaag tgaaaatata gcaattataa cagcaaaaag tgtaagacac tgtattaaga
     3601 gcatttgctt ttattcagat agttgtgata gagatatgac atctaatcat gaacctccaa
     3661 cagatggaaa acctagattt catcataatg taattataaa ttatagaaat atatcggcta
     3721 ttgtatgtga agataaacag ggagaatcag gtcattataa aaatctatct tttagtggtt
     3781 tttctggaat tgcacttgat atgcctaata attctacagc aaatgatatt acaattttta
     3841 catccggact tggaattagt acaggagaaa attcaactat tgctaacagt aaggtttggg
     3901 gctgtgttga tggaatgata atttctactg gaacttttat aaatggtatg agggtagaag
     3961 aaataaaaaa agttggaatt aaaaatattg gtagaggttt aaattttatt acaaatatca
     4021 caattgacca atgtggatat tgtggagttt ggtttgattc tatatccaat gttcagatat
     4081 caggaaatat cacaaggtgt gggcagtact attatgatgt aagctatgat aattatttaa
     4141 tgttggataa tagagttaaa gaagcatatt ctttattcta tggtaattta ttggaatcaa
     4201 gtaatatagt tttatcaaat accaataaag ataattggga agatttcaaa gaagataagc
     4261 ataaggtgta tcttgtagaa gcacttgaaa ccaagaatgt actattaaca tgcaatatag
     4321 atgctactga ctggattgtt aagtcagaaa aaggaaattt aacgtacaat aatatgcgac
     4381 aaactgttat attttacgat ggaaaactaa gtagtatcaa tggaattggt atatctgatg
     4441 aagattatgg ggataaagtc aagataaaaa aaggaaatat atatataaat agtaaggata
     4501 aggatattaa gatgcataaa ccagaggtag gtgacattat gtcaacaatt attaaagata
     4561 aaagtattct ttctgctcat tatgggggaa catgggagaa aattggtgaa aaaatagagt
     4621 ttggaataac tgtacactat tataaattga tagagaaata agtaaatacg tagtatttga
     4681 atagatgtat tttattggag agctaggact atagttttaa tgtaaatggt ttggtaatag
     4741 catggattga ataatataaa aataatataa aaaattaatt tgattatgta aaattctatg
     4801 aagtagtaaa actgtgactt tagtcatagg aggttcaaac taagtaaaag ggaaaggaat
     4861 atataatatt atggaaggaa gaactgggat taaagatgga agatggatta atgtagtaga
     4921 aagaggtatt aaaaatgatg gttcgtacaa cgtaggtcct gctttgcagg atattattga
     4981 tgaaatagaa acagattttt gtgtcttata ttttccaaaa ggtaaatacc tatttacaag
     5041 tggtgttaaa attaataaag aaattgtatt gcaaggtgat tctaattcat ctacaggcac
     5101 tacacaattt ataactcgtg gagtagaaaa tatgagtatt attacattga cagggaaaaa
     5161 acaatgtatt aaaaatataa atttttattc tgatagttgt gagatacagg taaataaaga
     5221 accaccgaca aaaggtaatc ctaaatatca ttatgagatg attattaata agaatgaaaa
     5281 gaatgttgag atgaataatg tatcagcaat cctgtatgat aacagtaaga atatgattaa
     5341 aggtctaggt cattatgaaa atatttatat aagtggtttt tcgggtacag gaattaggat
     5401 accatattac tcaattgtaa atgatattac agtttcatct tgtgggctgg gtattgatac
     5461 aggaatagac acaattattt ctaatagtag aatatctaag tgtcaaaatg gaatgaggat
     5521 tagaactgga actagtataa ataatgtaag gattgaaaaa atacagaaag ttggtcttat
     5581 aagtagtcaa gttaaggggt atgaggggta tggaagttat aaaattaata atattacgat
     5641 tgaccaatgt gggtattgtg gcttttcatt tgattcaatg aaagatagtt atttttctgg
     5701 tattataaga aggtgtggtc agtattatta taatacagat tatgacacat atttaagtat
     5761 taaagataga gtagaagaag catattcttt attttatgga aattatttta cagaatgtaa
     5821 tattgattta gttagtgata ataaggataa ctgggatgat ggcttagaat ataaacataa
     5881 agtttatgta tttaaaaccc ttcaaatacc aaaattaaca ttgagatgca atatagatgc
     5941 ctatgattat atcgttaaat cggtacatgg aggaggtaat ttaatgttaa aaaataattt
     6001 taacaattat atatttattg attcatctaa tccagaagtt attaatggaa ttaattttaa
     6061 ttatcaaaag aatggaaact tgttaaggtt aaataatggt aaattaagtt ataaaaatgg
     6121 aaaggaagaa acagctatat tcagacctaa ttatggtctt attgtagcat caacagattc
     6181 agaatgtgaa caaatagaga ctagctatgg aggaaaatgg gagaagcttg gttctgaagt
     6241 aattggagaa gaaacggtat attattataa acatcatgaa ccagataaaa caaaccaaat
     6301 acaataaaat ttgtattata taatgatata aagttttaat atatagtgtt tacctttaat
     6361 gtaatattgg tatatttgta tagtattaaa tttgttaaaa agtaaaattg tagggtgctt
     6421 gaaaatagca ccttttttag tttcattata aaattagttt tgaaagttta ggaataatgt
     6481 atatttattt ttaaagaatt ataaccttat taaggatatt ttaaatgcta gaaatgaatc
     6541 agtaaatcat actggaatta tcttaaatgt tttgattaaa ttaagtattt agagctataa
     6601 ttatattaaa aatatattag aacataatta tattactatg gaggttaaat atgatagtaa
     6661 gtgtgagtag aagaactgat atacctgctt tctatagtaa gtggtttttt aacagactta
     6721 aagaaggttt tgtttatgta attaatccaa tgaatccaaa acaggttagt aagatagagt
     6781 taaatccaca tactgttgat tgttttgtat tttggacaaa ggatgtaact ccaatgttat
     6841 atgatttaaa taaattaaaa gactataaat attattttca ctatactata acatcatatg
     6901 gaaaagaagt agagacatgt attttagata aaagaaaggt aatagattca tttaaggaat
     6961 tgtctagaaa aataggcaaa gaaagagtaa ttttaagata cgacccgatt ttcttaagtg
     7021 aaaagtacac agtagattat catatcaaag catttgcgag tttatgtaat cagcttgatg
     7081 gatttacaga aaaatgtata ataagtttta tcgatttata taagaaaaca aaatttaata
     7141 ctaaagcatt acatataaag ccaatagaaa ttacagaaat agaaacatta tcaaaagaga
     7201 tttctaagat tgctcataag catgggataa cacttgaaat gtgttctgaa gaatataatt
     7261 taagtaaatt tgggattgga aaaagtaagt gtattgatga tagattaata tcaaaaatta
     7321 taggatgtga agtagaggta aaaaaagata gtacacaaag agatatttgt ggatgtgtta
     7381 aaagtgtcga tataggacaa tacaatactt gcagacatta ttgtctgtat tgttatgcta
     7441 actttaatta tgcaaaagta gaagaaaact gtaggcttta taaagaagat aataaattac
     7501 tcatagatac tttaagagat gatgcaaaaa taagcattag agatatgaag actattaaag
     7561 tagataagaa taaacaaatt agtatatttg attaaaataa atatactaat taccttaata
     7621 tattaagaat atagatactt ttgataacaa aattaaactt aatagatatt ttctatataa
     7681 gaaagatggt cataatatgt caatttgacc atctttttga tataattaaa acacaaataa
     7741 tttagtaaca aaggaggcag aacttgaatt tattaaaaaa atatcctata cctattgcaa
     7801 gtttgatttt gtcaatcttt actttaggta acttattaca atcttatagt gaatcattac
     7861 gtaatgtaat tggatttata ggatttattt tgtatgcaat ttatatagtc aaaataatat
     7921 tacttagaaa gggtgttaaa gagcaattag agaacacttt aatatcaagt acatttccaa
     7981 ctataacaat ggcaacaatg atatttgcta catatataaa accattatct ttagaattaa
     8041 gtattatgat ttggagcata ggaattgtgg gtcatcttgt gttaatagtc ttatttagta
     8101 agaagttctt agttaaattc tcaataaaaa cggtatttcc atcttggtat atagtatatg
     8161 ttgggatagt tgcagctagt gttacggcac cagcatttaa tcaacttttg attggcaaaa
     8221 ttgctttttg gataggattt gtatcttata tagttcttat accagtaatt ttaaagagag
     8281 tatggataat caaggaaatg cctgaaccat ctttaccaag tatagctata ttagctgcac
     8341 cagggtcact attattgtct ggatatatta attctttttc agataaaaat aatgtagtat
     8401 tatacttgtt attcatatta tcaattgtat tttatattat agttttggga tatttaccga
     8461 aattattaag attgcctttt tatccgagtt ttgctgcatt tacatttcct atggcaataa
     8521 gtgcaatggg tatgaaacta atgaatggtt atttaactaa atcaggtcat tttataagtt
     8581 ggatttcata tgcttctaag ttagaagaga taattgctac agtgattatt ttatatgttt
     8641 tagttttgtt tattatatat cttagtaaat ctgaaactac atctaaagct aaataagaat
     8701 tattaaatat gtgtatttaa taggaatatt gatataaata tatgaagata agataaacca
     8761 tagaaactta atttagctta attttattgg aggaaaacaa atgaaaacaa aagaagaata
     8821 tttaaaagaa gttagaaaca tatcagaaga atactttaga aaaggagagt ttttttgctc
     8881 tgaagcagtt ctccagacaa taaatgatgc tttaggccaa cctttaagtc cagagataac
     8941 taagttagca tcaggatttc cgataggact tggaaaggca caatgtcttt gtggagcaat
     9001 atctggggga gagatggctt taggtatagt atatggaaga gttcatggtg aagctatgaa
     9061 tccaaagatg tttgagcatg ctaaaggttt gcatgattat attaaaaaag agtatggtgc
     9121 aacatgttgt agagctatta caaagaaatg ggatggtgat aactttatgt ctcccgaaag
     9181 aaaagagcac tgtattaaaa taacaggtca agttacagag tgggttgcag ataagcttat
     9241 agaagatgaa aaacttaata tcaaatagtg taatattagt tggtgaattt ataaagttaa
     9301 atttacttat agtataagat acttgatatt ctcgtattga tagaaaaatt tatagcatat
     9361 gtatttaatg aaatatactt atatttagag tgtggtttaa tttcataaat acatatgcta
     9421 ttattttaag tatataagca aatttattta aataaataga aacagttaat ttatatgtac
     9481 aaaactatat atgattagtg taaataaata cctattttat aatactcaac ataaagacac
     9541 cagacatgat tagtattata ccaataaaaa tagcaagcca ttcttttttg ttcttctttt
     9601 ctttaaaaaa tataatccct cctaaagtgg ctactatcac acaactctga gagatagaaa
     9661 aacttattgt aactcccaat gtattagtcg ttatgaacat acctaaattt gctattgacc
     9721 acaatatacc agtaattaaa ttgaataaaa cattttccct atatacaatc tctttagaaa
     9781 aatacttgac aataatagat gaagttatca acataccaac agattgaggt aatataattc
     9841 tatatccttc aattaaaaat atttgattag ttacaacata taatgataaa aatagagatg
     9901 ataataatac aactccaaca cattttaaca tatttttatc tgtagatttt ttagtttgat
     9961 aatttgtgag aagtatacca agtattatac ataatattga taaagaacca atcagtaaca
    10021 ttttagaact ggtccattca ttaaacaaga ttacagctaa taatgtcgta aaagttaatt
    10081 gtgaaccatt tgaaattggc attactttag atacactagc aatttgaatt gctttaaact
    10141 gcattaattg tcccaaactc cagaatatgc cagaaatcat acttatacaa aatgaaaaaa
    10201 atgtaatttc aggatgtata accagagtta ttatagtgga aaatataaac gcacttatgg
    10261 atgtacctaa aagctgttca tgaggagaac ttttctttag atttgcaact atgggcatca
    10321 atccccatcc cattacagga agtaaagcaa taattattgt cagcattaaa ctatttcctt
    10381 tcatattaaa tttagacagc ttttaattta tcatctaaaa ttaaagtgtt caatacataa
    10441 aaattaccgt atttaatcag gaaatattag tagtttttta gaaaaacatt ttccattaaa
    10501 ataaagacaa agggaaggag taaaattgaa aatgttaact gatatgattc aaaatcgtga
    10561 aattaaaata ataaagtttt tattaaaaga aggtcataca actgtaaaag caatagcaga
    10621 tgatttagat gtttcagaaa aaacagtttc aaaatctctt aaagaagtag aagcagtgct
    10681 taaaaagata ggtctttctt taataagaaa acctaagata ggtgtttata ttgatggaga
    10741 tttaaaaaaa atacttcctt acctagataa taaaggaagt caaattccta caaccagaga
    10801 ggagagagtt atctatatat attcagtact tttaaagagt aatgattata ttactataca
    10861 acaactttct gatgaactat atataagtcg tggaactatt gaaagggatt tagcagaagt
    10921 tgaaaaactg ctaaaaaacg aagggatttc catatataag aaacctagta agggaataaa
    10981 gttaaatata agtgaaagag ataaaagaca acttactgct aagtttatac ataaattttg
    11041 gagtagaaac tggtatatta agcaagagca agaaagcttt tatcaagcat ttgaaaagat
    11101 acaagcagat gtaaatggta ttttttcgga agatgattta tcaataatta ttgatattct
    11161 taggaatttc agcagagaaa gaaaatttga gttctctgat tatgaatttc agtcattagt
    11221 tattcatttg gcaatagctg tagagagaat tagaaaaggc gaatatatag aagaaagttt
    11281 gtatactggt aatatagaaa ataatcaaat gtcaaatacc aattatttag caaaacaatt
    11341 agaagaaaaa ttttcaatat tattaccaaa atctgaaatt ggatatataa atttacattt
    11401 agtagcgtct gaccaacata gagatactaa tgaactaaaa aatacttcaa ctaatccttg
    11461 tgagacaagt acaaataata tatcaaatct tcgggaaatt ataaaaaata cattgtatga
    11521 gacagcttat gacaaggatt taattgatgg attgacttca catgtacatt catcaataaa
    11581 tagactgaaa tatggactta gtattaaaaa tccatatgta aatacaatca aacaaaactt
    11641 ttctttatca tttgagcaag ctttagattt aaagaaagta attgaattta tatatgatat
    11701 taggataaat gatgatgaat gtgcttatat agcattacat tttgaagctt ttagagaaag
    11761 aatgaaagaa cttccacaaa atattagagt aattctagta tgcagtacag gattaggttc
    11821 atctaggtta ttgtctgcaa gaataaaaaa atactttcca atgataaaga ttgaaaaagt
    11881 actttccgta caagaattag tatccagtaa aattgatgca gatttagtaa taagtactat
    11941 ttatttagag ttagaaacaa ttccaaccat agtagttagt cctattttaa atgaaagtga
    12001 tataagaacc ttggaaagaa atatacaaaa tgtttcaagt tgtacttcta agaagtataa
    12061 atctttcaga aacttaatct ttgaagaaaa tatattgttg aatattgaag cttcaaacta
    12121 tgaagatgca attaagtata taacagataa cctaataaaa aaaggttttg caaaagaagg
    12181 tatagctaaa agtgcaattg agagagaaaa tttatcttac acttctttcc aatcaatagc
    12241 tacacctcat gctaatccta gttatattaa aaaatctaat atatctgttg caacgttaaa
    12301 aaatcctatt acatggggaa atgaaaaggt ggaagttatt ttctttattt cattagaaaa
    12361 tgacaaaaac ctagagcttg atgaaatata tgaagttttt tatgacttta ttgacaataa
    12421 aaaaaatata aatgacatat taagtgcaac ttccacaaag gaagtgtacg atttgctagt
    12481 ggaggagagt atatagtgaa aatatcagat gtattgaaaa aagagcaaat tgtttttaat
    12541 cttgatacaa atacaaagga agaaacaata aaaaaattaa gtagtatatt tgttgaaaat
    12601 gggattgtgg acaatgaaga agaatatgtt aagtctgttt tggaaagaga agaacattca
    12661 actacaggta ttggtaatgg tattgctatt cctcatggga aaagtaatgc tgtcaaaaaa
    12721 tcttctataa tatttgcaaa gactaagcat aagattgaat gggatgcttt agatgaaaaa
    12781 ccagtagata gtattttctt attggctata agcaatattg ataaggatag caatcatctt
    12841 gtactattat ctaaattagc aactaaactt atggatgatg acaatgtaga tgcactaaaa
    12901 ttagccaatt cagaacaaga aataatcaat atatttagtg aagaaggaga gatgtagaaa
    12961 tgaaaagaaa aattatagca gttacagcat gtgcaacagg agttgcacat acatatatgg
    13021 cagctcaagc attaaagaaa gcttcaaaaa agatgggtca ccttataaaa gttgaaacac
    13081 aaggagctac aggaatcgat aatgagttaa ctacaaagga tgcacaaatt gcagaagtag
    13141 taatatttgc agtagatact aaagtcagaa atgctgaacg ttttgaggga aaagaaatac
    13201 tagaagtacc agttaatgca cctataaagg atgcagagaa agtaataaat gatgcattaa
    13261 agttaattga cactaaataa atggagggaa atgtaatgtt gaaaaagata gtctctgagt
    13321 taaaaaagca tgtcttaact ggaatctcat atatgattcc attagtaatt gcaggtgcta
    13381 tgataatggc gatatctcgt gtaggtggtt ctttctataa tataccagat atatgggatg
    13441 ctaaatatgc agaaagtgct agtagtattg ttagattatt acatgattta gatgggtttg
    13501 gaggtactgc attaggatta atgtttcctg taattgctgc atttatagga ttttctatag
    13561 ctgataaatt agctattgta ccaggtcttg ttggaggtat gattgctaaa gatataggtg
    13621 caggattttt aggagctttg gcagttggtc ttatagcagg atatacatgt ttgtttatta
    13681 aaaatcatgt taaattacca aaatcagcag cttcaatagt accaatattt atagtaccag
    13741 tatttgggac attaatcact gttgtattaa tcaattatgt gataggtata ccttttgcca
    13801 cattaaatac tggattagaa aattggttaa atggattatc tggtacaaat cagatactaa
    13861 tggcagctat tataggggct atgatggcag ttgacttagg tggaccagtt aataaagcag
    13921 ctttaacaac atcacttgca ttattaacaa gtggaattta tgcgcctaac actgctgcaa
    13981 tggtaggaat agttatacca ccactaggat tgggacttgc tactatacta gcaaaacaaa
    14041 aatataataa acagttacgt gaagctggaa aatcatctct tataatgggt ttaattggag
    14101 taagtgaagg agcaattcct tttgcggtag aatctccatt aaaagtaata ccttcatgtg
    14161 taattggaac agcaattgct tcagctatgg cagttggttt aggttcagta aatgcaactc
    14221 caattagtgg attttatgga tggtttacag tagagaattg gccaatgtat gtgttatcaa
    14281 tagcagttgg aacagtaatt gtagctggat tagttatagt attgcgtgga aatccagtga
    14341 ttgaggatga tgaatttgaa gatgatgatt ttgatgaagc agagtgggaa tcttaaaatt
    14401 taataactat ttaagacgtg ttataagcta tattcaatat gaatcttata ctaaataaag
    14461 tgttaaatat tcagtttaga cttaaatata aaagataaag aacagaggag aataaatgat
    14521 aaaagcacat attgttaatc atacacactg ggatagagaa tggtatttta cttctggaga
    14581 tgcattagta cttagtgaac aactatttac agatgtaatt gatgaattag aacgtaatcc
    14641 agatgtaagt tttgtacttg atggacaact ttctattttg gatgattatg tacaattaca
    14701 caaagaaaaa atagaaatca ttaaaaagtt aataaaagaa gatagactac atattggacc
    14761 atggtttact caaactgatg cattctttgc tagaggagag tctattttaa gaaacttaat
    14821 gattggaatt tttgaaagta aaaagtatgg taagtatatg aaaattgggt atttgccaga
    14881 tacatttgga tttaatgcac agatgcctat actattaaag catgtaggtc ttgataatat
    14941 attcttttgg agaggtatac atcttggtaa acaggttcaa tcaccatatt ttaagtggaa
    15001 aagcttaaat ggagaaagtt atatatatgc agtaaatatg ccacatggat atggaactgg
    15061 tatgctttta gagccttcat taaagtatgt taatggacgc ttagacccag ctattgattt
    15121 tattaaatca tatactgatg tagatgaggt gttaatacct tctgggaatg accagttaaa
    15181 tataataggt gatttcaaaa ataaaattaa agcgattaat gatatcggaa agtattcata
    15241 tataacgagt tcatatcaag agtttttaaa atatataaag tccatagaat cactagaaag
    15301 ctatagaggt gaatttagag aacctgtatt agcaagaata cataaatcaa ttggttctag
    15361 tagaatggat ataaaattag catgtgataa actagaaaac aagctgatta aaagaataga
    15421 gccattatta gtaattgcta aaaagagcaa aattgaaata agcaatcagt tacttataaa
    15481 tacatggaaa aaacttttgg aaggacaagc acacgatagt ttagctggat gtgtaactga
    15541 tactgtaaca gatgatattc ttcaccgtat aagagaagca aatgaaatat gtgacagtat
    15601 tgaaaatact attgtaaaaa agatttcaga agggcttaaa ttatctaaaa atgatatttt
    15661 agtatttaat acagaagcta agcgttttaa tggatataaa gagatacaag ttgtaagtga
    15721 tagtaaaaat atttatttta aagataattt agatgctact ataattgaag aaacttatgt
    15781 aaaaccgaga gaaaatgtat tagaagaaac acctgctggt aatatattta ttgaagaacc
    15841 aggatattat atattaaaag taagaataaa tgtacagctt cctgcacttg gttataaagt
    15901 tgttagtttt gaattaagtg ataaagaaat ggtgtcactt gataaatcta atgacacttt
    15961 tatatctaat gataattata agataatata tgaagatgga agcttaaatt tacaacttaa
    16021 agatggaact tacatatcaa actttttaac attaaaagat agtggtaatg caggtgatac
    16081 ctatgacttt tctccactaa aaaatgatga gaatataaaa ctttcgtttg ataaagtgta
    16141 tacagaaaaa gctttaggat atgaaaaaat ggtggttaaa ggtactactt tactaccact
    16201 tacattagaa gatagaaaaa acaaatatct taatggtaaa ttaaatgtaa aagtaagtat
    16261 tgtcttatct aaagaaaacc cattgatgga tgtaaaactt tgtgttgata acacgatata
    16321 taatcataga ctacgtgttc atataaaaac tgatataaaa gataataaaa acatagcatc
    16381 attgccattt ggatatataa caagaaaaaa tggagtttta gacaactggg aagatattta
    16441 cagtgaaatg cctatagatt tagaaccttt ggaaagtaat attacgctta caaaccaaaa
    16501 tagaagttgt actgtattta ctagaggaat aaaagagtat caacatattg aagatgaaat
    16561 tgcattgacc ttacttgcta ctacagatga actaggaaag cctgatttat tatatagacc
    16621 aggacgtgct tctggtgata caactaaaaa aggtcatata agaattaaaa cagataaagc
    16681 tcaaatgctt aaggaattag aattttcatt tgcaatatat atggattcta aatcatttga
    16741 tgaattggaa attgcgaatt taacccacaa ttatctaagg gaatgtgtaa attatcaatt
    16801 acaggattac aatttctttt tatatcatat agataataag attcaaaaat caattgtcaa
    16861 taaaactgta agtagagaat tagaaataat atctttacca gaaaactatc ttatatcagc
    16921 atgttatcct tcttattaca gtaaagataa atttataata agagtggaaa atccaagtag
    16981 taatagaatg atattggatg agcaaatatt taaaaataga aatgggaaaa ttgtaaatgc
    17041 aatagaagat gtagaagagg aacaggtata tgaaatagca ccttttgacg ttatttcaat
    17101 attattggat ttataagtgt aaaagtaagt ttattgttta aagtctgaca gatattaatc
    17161 tgccagactt tttaatttgt ataaatttgg gtaaatatta atgaatggtt ttagtattta
    17221 agtatgaatt tagtatttaa tatagaattt attgaagtag aataattagt ttaacaggat
    17281 aagcagttta aat
//
LOCUS       DAMKMI010000087        17230 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.088, whole genome shotgun sequence.
ACCESSION   DAMKMI010000087 DAMKMI010000000
VERSION     DAMKMI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17230
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.088"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(176..700)
                     /locus_tag="PC429_002501"
     CDS             complement(176..700)
                     /locus_tag="PC429_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3848001.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(765..1517)
                     /locus_tag="PC429_002502"
     CDS             complement(765..1517)
                     /locus_tag="PC429_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF3848002.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1548..2846)
                     /locus_tag="PC429_002503"
     CDS             complement(1548..2846)
                     /locus_tag="PC429_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF3848003.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2863..5127)
                     /locus_tag="PC429_002504"
     CDS             complement(2863..5127)
                     /locus_tag="PC429_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF3848004.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5382..6200)
                     /locus_tag="PC429_002505"
     CDS             complement(5382..6200)
                     /locus_tag="PC429_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF3848005.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6193..6891)
                     /gene="cbiQ"
                     /locus_tag="PC429_002506"
     CDS             complement(6193..6891)
                     /gene="cbiQ"
                     /locus_tag="PC429_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF3848006.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6894..7172)
                     /locus_tag="PC429_002507"
     CDS             complement(6894..7172)
                     /locus_tag="PC429_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3848007.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7162..7914)
                     /locus_tag="PC429_002508"
     CDS             complement(7162..7914)
                     /locus_tag="PC429_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF3848008.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8096..8288)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8420..10192)
                     /locus_tag="PC429_002509"
     CDS             complement(8420..10192)
                     /locus_tag="PC429_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF3848009.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10206..11135)
                     /locus_tag="PC429_002510"
     CDS             complement(10206..11135)
                     /locus_tag="PC429_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3848010.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11364..11552
                     /locus_tag="PC429_002511"
     CDS             11364..11552
                     /locus_tag="PC429_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3848011.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11545..12246
                     /locus_tag="PC429_002512"
     CDS             11545..12246
                     /locus_tag="PC429_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848012.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12248..13171
                     /locus_tag="PC429_002513"
     CDS             12248..13171
                     /locus_tag="PC429_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3848013.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13328..14251
                     /locus_tag="PC429_002514"
     CDS             13328..14251
                     /locus_tag="PC429_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848014.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14244..14981
                     /locus_tag="PC429_002515"
     CDS             14244..14981
                     /locus_tag="PC429_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848015.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14992..15765
                     /locus_tag="PC429_002516"
     CDS             14992..15765
                     /locus_tag="PC429_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848016.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAWGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16306..16914)
                     /locus_tag="PC429_002517"
     CDS             complement(16306..16914)
                     /locus_tag="PC429_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF3848017.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17092..17208)
                     /gene="rrf"
                     /locus_tag="PC429_002518"
     rRNA            complement(17092..17208)
                     /gene="rrf"
                     /locus_tag="PC429_002518"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5552 a         2994 c         2173 g         6511 t
ORIGIN      
        1 taaaacttta ttttttagtt aaaatcatac aatatataga tttccacaat acaaataaat
       61 taaaaagtga aatgtatttg tctaaaaaca taataacatt tcactttatc ctcattttta
      121 agtaatatat atttttaatt tttatttata actaaatcta gtttttatat aaattttagt
      181 ttttttctac atctgagtaa tctattccaa aagtttctac agtaacagat ttcataattt
      241 gtggagtttg tggcttatca gctgaatctg ttctaactga tgctatttca tcaactgtat
      301 ccataccttc tatcactcta ccaaaacctg catactgacc atctaagtgt ggtgagtttt
      361 tatgcattat gaaaaattga gaaccagctg aattaggtgc catagttcta gccattgata
      421 tgactcctct ttcatgtttt aaattgttag taaatccatt accactaaat tcacctttta
      481 ttgagtgacc tggaccaccc attcctgtac catttgggca tcctccttgt atcataaatc
      541 ctggtattac tctatgaaat attattccat tgtaatatcc ttcatttact aatgatacaa
      601 agtttttaac tgtatttgga gctatatttg gatataactc aattttgatt tcttttccat
      661 tttccatttc tatagttact ataggatttt tattttccat aatttattac cttccttttc
      721 attagttaaa ttacataatt acatttatta tattatactc taaattaaag tctatcaagt
      781 atattttttc ctatttcata tccttcttga taaatagttt tacaaaactc atcatcaaag
      841 ttaagcgtgt ctttaggagc aaattgagta ttaggtctaa ctactattat tttatcttca
      901 tcatatactt ttttaatatc ttctggcaac tcataattat attttgtgga tattaaaata
      961 attttatcca cattatcccc aattaatggt tcaattaatg cactcctaat taaacctcca
     1021 tctaatttga aaccatcata aattccttct tgcacatctt tcacaaattg ttctttaaat
     1081 ccaagagttg cgtttggatt tgatggtaac aacccactgt atgaaatgct gataatggct
     1141 tcattacgag gcatttttga tacatcaaca atcttttctg atactacttt attatcaact
     1201 tcaacataat taacataaaa gttcatttct tctatattac tatcatctag ttcccttagg
     1261 ttgtctatag caggagaatt atccacagta ttatttacta tttttacatt tccattctgg
     1321 atatctttta tatttgtcca cattttctcc aagttatcaa cattccctgt gtacaaataa
     1381 tatccattta ttgctcctat tgatgtccct gaaatcgaat aaaatttatt tattcctcca
     1441 tcatacaagg ctttaactac tcctgcttga taagcccctt ttgctcctcc accttctaaa
     1501 cataatccta atttcatagt ctcatctcca aattgtttat cttatattta tatttcaaaa
     1561 aacttactaa atgttttata tatactatag tggtcctcaa catttcctaa ttctcttata
     1621 gaatgcattg ctaaaattgg acttcccaca tccacagatg gtatatctat atgagtagaa
     1681 gatattggac ctatagtact tcctcctctt tcatctgacc tattaacaaa ttcttgatac
     1741 tcaacattag cttctctaca tatattttta taaatagcta tagaatatcc atcactagta
     1801 tacgcctgac tagcatttat ctttataacc ggaccttttc ccattattgg tttattagtt
     1861 gggtcatgct tttctggaat gtttggatga atagcatgag ctaaatctga tgatatcata
     1921 aaagatgaat aaatagactc aaagaatccc tctctatcct tacccattga tatacaaatt
     1981 ctctccaata tatttaacaa catgtttgaa tctgcacctt gtttagtaga gctaccaact
     2041 tcttcattgt caaatacagc cattacattt acaccatgtt gacctttagt attaacaaga
     2101 gcattcaaac tagcatgtac cattgcaaga ttatcttgtc tcccaataga tataaattca
     2161 tcattagccc ctattaaact tcctttttca aattcatata aaaataaatc aaaatctata
     2221 atatcgtcca tatttacatc taagttagat gctatttgct ttataagaaa attatctttt
     2281 tctaatgtat catttaacat cccaactaaa ggtaacatat ctttttgctt attatatgaa
     2341 tgaccatcat ttatacttct atttaaatgt attgctagat ttggtattat acaaattggc
     2401 tttttaaagt ctataagcct ttcatttggt tttaatatac tatcaccttt taaaactact
     2461 ctaccagcta tcccaagagg tctatccaac catgtactta atattgctcc tccatagcat
     2521 tctgtattta acttcaaata agatttttca gcttctattt ctgcatttgg tttaatacta
     2581 aatgtaggag aatctgaatg agagcctatt attctaaatc catctctttc tatatcttct
     2641 gaatttacta caaaagctac tatagctgat aaattcttag taacataata ctttccacca
     2701 actcttagat tccatctttg attcaaaact aattcttcaa atccattttt atctaagatt
     2761 tccttagtac tcttgactgc atgaaaagac gacggactat catatatgaa gtctattaag
     2821 tttttagcaa agtttatact atccattcct catttacctc cattataaaa tatttagtgt
     2881 tgtatattct ttatttattt ttttaattcc ctgttggtca aaagctatag taacatctgt
     2941 accaattata gaaaccactg tcccaacccc aaatttagga tgatgtacct tagaaccaag
     3001 ttttatatca tctggattag tttctttatt agaatctttt attgtagcat taactttatc
     3061 tgcaacttta gatttgctta tagtactcat atacttttta gtatatttat ctaacatatt
     3121 ataacttgct ttagaatagc ttagttcttt ttttactttg tacaatcttt ctatacattc
     3181 ttctggcagt tcttccataa atcttgaagc tattgcaaca tttgttttac catataatgt
     3241 tctcttctct gtcaatgaca tatatagctc ttctttagct ctagttatac caacataaca
     3301 aagtcttctc tcttcttcta tttgtgaatc actcatagat tttatagctc ttgatatagg
     3361 aaataaccct tcttccatac ctgttaaaaa cacaacaggg aactctaaac ctttagatgt
     3421 atgaattgtc ataagcgaaa ccctatcatt gtcttcttca tcatttgatt cagatgtaag
     3481 tgctatactt gttaggaatg tctctaagtc tttattttca taagtatcat cattactact
     3541 ttcaaactcc aaagctatag aaataaattc tctcaaatta tcaattctgt cttgagcttc
     3601 ttcacctttg cctgttaaat ctttttcatt tttatttctt atctctacaa gctcgtccat
     3661 atatcctgta gtgtccaaga ctttttctat aagtttgcta acaggataaa cttcttttat
     3721 agttctaaga gtccctataa tatcaacaaa tccacttata cttgcccttg ccttagtaga
     3781 tatatctgaa tttgtttcta tatcaattaa tactgagtat atactttcct gttttaagtt
     3841 agctctatct tcaatttttt ctatggttct aagccctata cctcttctag gaacatttat
     3901 aatccttttt atagatatat catcttgtgg attttgaata actcttaaat acgccaacaa
     3961 atcctttatt tcttttctct catagaattt tgtaccacca tatatattgt atggaatttg
     4021 acttctattt aaagcgtcct ctacaggtct tgcctgtgca tttgctctat aaagtactgc
     4081 aaaatcctta taaggtctat tttcttttct agctatttgt gcaattgaat cagcaatgaa
     4141 atcagcctct tctatttcac taccagttaa ttgtatcttt ataagttctc cttctttttt
     4201 ttcactccaa agcttttttc tttttctttc tatattatta gatataactt tattagctgc
     4261 atctaatatc acttgtgtag acctataatt ttgttccaac tttacaacat gtacattgtc
     4321 ataatctttc tcaaattcaa gtatgtttct tatatctgca cctctccatc cataaatact
     4381 ttggtcatca tctccaacaa cacaaatatt ttgatgttct cttgctaata attttatcaa
     4441 ttcatattga gctttacttg tatcttggta ctcatctacc attatgtatc taaatctact
     4501 tctgtaatat gctaacactt catcattagc cttgaaaagt tctacagttt taagtattaa
     4561 atcatcaaag tcaagtgcac tatttctttt taaacggtct tgatatagcg cataaatatc
     4621 tgcaatctta accattctat tatctgccat attgatatct ttaaactgct ttggagtata
     4681 aagtttatct tttgctcctg aaattgcact tataactgct tttggttcaa acactttatc
     4741 acttaaattt aattctttta gacagtcttt aaccaatgta acttggtctg ctgaatcata
     4801 aattacaaaa cttctattgt aaccaatctt atttatatct tttcttagta tacgaacaca
     4861 gcaagaatgg aatgtactta tccacatatc cttagtttca ggacctatat tttgttcaac
     4921 tctctctctc atttcatttg cagctttgtt tgtaaatgtt atagctaata tatttggtgc
     4981 ttgtacacct ttatcttcta taagatatgc tattcttgtt gttaatacct tagttttacc
     5041 tgaaccagca ccagccagta taagtactgg accttcagtc ttttctactg cttctctttg
     5101 agcaggattt aatgtatcta aattcatatt tctcctcctg ttaacagcca taaatatatt
     5161 ttaatctaaa atatgaatta cttcaaatta taattatgta ttatattaat agattatttc
     5221 caatatatta caattaaata acatttttcc atataattta tttcattgtt taataaaaaa
     5281 aataacctta tgatttgggg aatcataagg cacctttaag gggtctaggt taagggttat
     5341 tatatataaa tctatttttt taaaataggt ttagggctta attatttatt tgtattaagt
     5401 tctctttcct tccaatactt atacaaatct tcttctttct taaataaggg taaattcata
     5461 tttctatgta cttttactaa ccatggggat tctaggccag cttgtataat attattttca
     5521 tttataaaca cattttctgc ttttccttca tcaatcaaaa tccccttatc caatacatat
     5581 atgtaatcac atatttcata cattaaattc atatcatgac tggatattac tatttttata
     5641 ttatttttat ttaatccctt tattatatct acaatagacc tagtacttac tgggtctaaa
     5701 ccagctgtag gttcatcaag taatacaatt tcattttcca ttgctataac tgatgctatt
     5761 gccactcttt ttttctgtcc atagctaaga aaatgtactg gcctgtctat aaaatctatt
     5821 ccattaacag ctactagagc tttgtttatt ctctctttaa ttgttttttc atccatacca
     5881 atgtttctca ttgcaaaggc tatatcatca tatacttttg aataaaaaat ttgtttttct
     5941 gggtcttgga acacaatacc tacatttttt ctaaggttgt ataaacttct cttatcatat
     6001 tttaactttt cttctttaaa aagaatttct ccagatgtag gctttaatat acccattaaa
     6061 ttcataaata atgttgattt tcctgaacca tttgaaccta ttatacctat aacatttccc
     6121 tttgaaaaat ccatattaat gtttaatagt gcattggtat ttttttcata ttgatacgtt
     6181 aaattattta ttttaaacat cattatcccc tactatgtga aatttaccat catacaattt
     6241 tatgtctaat gatatacaca tatcatcata ccttgtcata agtcttttgt acagcatact
     6301 tccaagaata cctaaggatt tatatgaagt ttttaaattt atatatccaa atcttagctc
     6361 ttgtgacttg tatatttctg aaaattcttc taaaaatatg aatataaacc tatatattaa
     6421 cattgataac tcgacaaaag tgtcaggtaa atgaagtttt ttaaaaaaga atattaattg
     6481 attaaaaggt gttgtaagta tacaaaaata cacacaagtc aaacaagaca tcgctctaaa
     6541 taacacatga atagacatgt caatagacgc ctttgatatt cctacattaa aactaaaaaa
     6601 ttcaaaacta tataataaat ctgctttatt aaaagatata tttacaagag ttattcctat
     6661 actaagaaat aaaaagtaca ttggtattct aagtaatttt aaataatttt ttaaatctat
     6721 tccagccaca caaacaacca aaatactcat taaaatcata attccactta aaataaatat
     6781 attttgtatt accatagaag caatcagaaa aattactcct atggctactt ttttgtttgg
     6841 atttacttta ctcaatctat ttgtatatgc atatttatca attataagca tgattaactt
     6901 ctctttcctt tttgaagacc taatatgtat cctattacac cagcccctaa agctgcttgt
     6961 gcagagaata ataaactttc tatttctcca cttggtggct catatggact gctaaaccat
     7021 ggtttgtaat ttggatttat cttagtaatt tcactttcag cttgaccatc tgctcctcca
     7081 tattcagcac ctgagttgac aagtaatggg aatataataa gagctactac taataaaagt
     7141 aataatatgt tagtttttgt tttagcactc attagcttta actcctttat cattattttc
     7201 tagtatgctt ttttctccgt tttctgtaat taaattatat acaattacag taagtatacc
     7261 ttctgctatt gctattggta tttgagtcat aaagaaaatt cctaaaaatt taattgctga
     7321 agccattacc ccacctgatg ggtctgggaa agccagtgct aattgtattg aagttattgt
     7381 atatgtggct aagtctccta ttgctgctgc aagaaatact ggaatagcat tatttccatc
     7441 ttttttcttt aaagccttaa atattaggaa tgatacgatt ggccctatta tcgccattga
     7501 aaaagcatta gctcctaaag tagttattcc tccatgtgct aataacaatg cttggaatat
     7561 taaaactata gttccaagaa caaacattac gcttggtcca aacattattg ctccaagacc
     7621 tacaccagta ggatgtgaac aacttcctgt tactgaaggt atttttagtg ctgataatac
     7681 aaacacaaat gctccacata aagctaataa tactttcttt ttagggtctt gctttactat
     7741 tttacctatt gattttaatc cgaataagaa aaatggtata aatataactc cccatgctat
     7801 agaccatttt actggtaagt aaccttccat tatatgcatt gcatttgatg taactggagt
     7861 taaaatgatt aacataagtg cagctataac ccctaattta atattctgtt tcatcttagt
     7921 atccctctct ccctttctaa taattaaaac ataataatac atgtctgatt tatttaataa
     7981 aataaaaaag cctaactcga tggttaggct tctaattttc cacaatctaa atacaaacac
     8041 ataaaacaaa catatctgta taattattat aagaaacttt ctccatcccc gaagaaatta
     8101 ctttaatatt ttaggcaggt ctcctgactt agcttcatcc tactcctctt ccttcccatg
     8161 agatataaaa aatatcatca tagtggctaa caaaagtttc gtctgcatca cagtagcggg
     8221 ggctgtaaag gaatttaacc tttttcccta ttaatcttgc ttaaagaacc tataatatta
     8281 tatatttaat ttttaaattc ttcttaaatt attttctata taaatcattg tatacctttt
     8341 ctgtgaaaat ttctacattt tttataaatt ttattcattt ttatattttt atgtatataa
     8401 attctttttt gtaagtttat taatttctaa ttatcttctt taaatacatt gaaactacat
     8461 cagaaaatct agttatatct cttgaatata taaaatcatt tccatatatt aatctttcag
     8521 cttcaagttc tgattctttt cctgtaaata ctcctaatac taatatattt tgctttctag
     8581 cttttctcac ttcattggct gtatctctca aaccaactat acccctataa gccttttctc
     8641 ctcttaatgt tctctcacta ctttttccta tctttacatc atttggtctt ccatcactga
     8701 gaactatcag gattttattt tcttcatctc tatttattag accactacat atagatttta
     8761 ttgccaaacc atctctatta ttaccagcac aaaaatattc aaatatattt tcactatttt
     8821 ttatactatc atcataatct ttaaatcttt ttaatattgt atagtctaaa aaactagaaa
     8881 atcccatcac tctatttggt atgccaactt ctgttagagc atttgcaatt atgtaagctt
     8941 gaattgccac atttccctga tttctacttt gagagccact tgcatccaat aatatatcaa
     9001 taacgtattt ccctttttca ttttctatat ctttataaaa tattttatta ttattacttc
     9061 taccaactct ccaagctcta ttggctaata tagtaccaca atcagaatat actctacttt
     9121 tagcattttc ttctattaaa attcttgata gactttcttt taatctcttt atttgctgtc
     9181 tatacattct agcatgttct ctaaattttc ctaaattatt ttctttttgt cttgttacat
     9241 attttagttg aaatacatta ttacactcac ttctaagaac tccatctgta aagtgtattc
     9301 tacaaccttc atgaacattt ctacaatttt gtgtctcaat ttttttaatt tcacctttac
     9361 tcaaataagt ttttccataa taatgttcta tttttgaata tatcttattt gcagtgtctt
     9421 catcaacata tataactcgg tctgctgaat tgctacattt agaatcatca aaatccccta
     9481 caccttctac aagcatggat gaagatattg aatctatatc actttcaata atattttctt
     9541 cttcatcact ataaagttct tcatacatga aatcagcaaa tgtatcaaaa tttacttcta
     9601 cactttctac attattttca tcaataagac tattttcttc tccatttaat atgtattcaa
     9661 aataagttaa atatataaca tctaatgctt ttaaaatctc cataatatct tgattggtat
     9721 ctatatttct tatatcatta actattcttc tagtcatact atctaatgct gggtgcttgt
     9781 ccatactata aagtacaaat gtgtatttaa ttttatccat aatattatca ttgtgtattt
     9841 tggtaaaacg tgaaacaata tctttataag catttcttct tatgtcttct atccctggcc
     9901 tatctttaat gaccttttct tcaactattg tatttaatat aacttgaatt agacccagta
     9961 taatatcttt gttgtatcct tttcttattc tatttgtaag atatttttta actatatccc
    10021 aatctacata ctttcttcta gcacctgcaa ttattgcaaa atatagacct atttcttttg
    10081 aagagtaatc tttttctgat atatctatac tttcattgta atcaccagct attgtccatg
    10141 ataagttatt agctctattt tcaaattcat aatcatcata tatccatttc ataatctcac
    10201 ctatattaaa tatgacttat tggaaacaca tctatactct cccatttatt aggtattcta
    10261 gttttaatta catctccaac catttctttc tcatatacat caaaagtctt tcctgtaagt
    10321 cccatattaa ttgcaagagt tggttttaat cctcttctta tagtcttaag agatgccata
    10381 agaccacgta aatcaatagc tttactagat atctctccat tttgagactt caattgtaaa
    10441 tctaaaaata ttcctgcaaa gtgtattagt ttttcttcat cagcatctga aaattcattt
    10501 tttaaaatca tcattaactt atcttcttct ataggaggaa tatctattac cataaatcta
    10561 gataccagtg cttcattaag ttcttttgtt cctgcatatt cataattcat agtcccaata
    10621 aatcttgttg ctggatgtag attgactctc tcatacccag gtacatcaat tattcttcta
    10681 tagtctaaag ctgaatgtac cacaactata gcatcatttt ttgccatatt aatttcatca
    10741 aacaccccaa aacctccatt aatagcacac tcataaactg agcctcttct aagtttaact
    10801 tcattgtcta taaatgtatc tgtacctata agtgttgaac tgtctgtatt tatatgaaat
    10861 gatgtattcc actgaggtct attaaatatt tcccctagat tatcagcaag taagtttttt
    10921 cccgttgcct ttggtcccga taataatata ttttcttctt ctaaaatagc tgctatacac
    10981 atagacaaga tatctttacc ataaaaataa gttttagact ttgttactct gtattctaaa
    11041 tctttttcta atccataata atttctaaaa tacagtacat cttcaataag tttttcatct
    11101 accccttgca cccttagatt atctattata tccatatttt tacccctttt atataaatag
    11161 tatttgtttc atttcttatc accattttag cataaaagga tataagctaa aattgatttt
    11221 ttaatctccc ccattttaag taaaaccatg caccatttta tcactacaaa attaagagta
    11281 ctacttactg atatttcttg attttattcg tctatacgta tataatatat actgataaat
    11341 tatgaggaaa ggatgattga aatatggaat atatgtcttg cccagaagta gcaaaaaaat
    11401 ggggtatttc tgaaagaaga gtacaaaaac tatgtgaaga aagtcgcata ccaggagttt
    11461 caaaactcgg gtatatgtgg ctgataccaa agaacgcaga aaagcctgtt gatggtagaa
    11521 caaaaagaag aaaggaagta caatatgaat agcgttttga ttatagatga tgacaaagaa
    11581 ctttgtgctc ttatgaaaaa atgtgttgag caagaaaatt tatctgctat tactgcacat
    11641 ggtggtgcag aaggtctaca tttagtcgat aaaaatagac acacactctc tcttgtaatt
    11701 ttggacataa tgatgccaga tatagacggt tttcaagttc tgaaaaaaat tagagaaaca
    11761 agtaatattc cagtaattat gcttactgct aaaaatgacg aggatgataa agtttccgga
    11821 ctacgtttag gtgctgacga ttacctaaca aaaccgttca gcataaacga acttatggct
    11881 cgtgtcaatt ctcttattcg tcggtatact atgctaaacc ctgcttttat aacagacaca
    11941 gatttcataa taataaatgg catgattatt gacaagacaa accgaattgt ttctgtcaat
    12001 aatattcctg ttcaacttac aagcaaagaa tttgatttac ttgcttttct ggcttctaat
    12061 aagggacggg tgtttaccaa aaaacagatt tatacacaag tatgggaaga agaatatgct
    12121 tttgacgata gtaacatcat gtctttcatc agtaaattga gaaaaaagat tgaaccagac
    12181 cccaaccatc cattttatat tttgacagtt cgtggtgtcg gttatcgttt taataaggag
    12241 gcttaacatg aatacaagtg tctatctgct tttgatattg gctcttagcc tgattattat
    12301 agcgtacctt gtttctgtcc tgctacgtgt ccgaatacaa cttacactta taaaagatgc
    12361 tttagaggat ataaaaaatg gaaaccttaa tcgacgtatt ttaacagatg aaaacgacat
    12421 tacaaaacaa atttgctatg atattaacga aatagcaatc aatagccaat ttcaacttat
    12481 tcagcaaaaa caatcagaac aagcctacaa acagctaatg acaagtctct cccatgatgt
    12541 aaaaactcca cttgcttctt tggtcggtta tttggaagca gtagaaagta aaattgttgt
    12601 aggagaggaa aaagacgagt atatccatgt tgcgtctaat aaggctcatt acttaaagaa
    12661 ttttgtagaa aatctatttg aatgggtaaa attggattct aaggaacaag tttttcattt
    12721 tgatattttt gacttgaatg aattatcccg taatattata tctgattgga tacctgtttt
    12781 agaaagcagt cattttgaat atgaatttga tataccagaa atagagtact tcttacgtat
    12841 agacgcaaat gcatataccc gtatcattaa taacctatta caaaatatta ttacccatag
    12901 tagtggaaac aaaatgacat tgagaatttt tgaaaacaaa gagcaagctc aaattataat
    12961 aacagataat gggaaaggta tatcttcaga caatctccca catatttttg aaaggttgta
    13021 ccaatgcgac cattcacgag cttcaaaggg aaatggttta ggcttggcga ttgcaaagga
    13081 acttattaac gctcataagg gaaatattac cgccaacagc actcccggta tgggaacaga
    13141 atttacaata ttgcttccta aagccctgta aaagtacgag gctttcttac tctaaaacaa
    13201 gataaaagca agattatggc aaggttttgg caaggttcat gttttataat tctaattgta
    13261 gttgattttg acgttgtatc aactatcttt tgtagaaaca aatttattaa aagtgaggaa
    13321 attatatatg aatgattttg tgattgaaac aaagcaactt acaaaaattt atgggaatca
    13381 aacagtagtt gacaaagtca atctccatgt aaaaaaaggc cgaatatatg gtctgttagg
    13441 acgcaacgga gctgggaaaa ccacaattat gaaaatgatt ttaggattga cttcaatcac
    13501 gtatggcgag gtagatgtat ttggagagaa tattaaggga cgtgaaaaac aagtatatcc
    13561 ccgtatcggt gcaattatcg aaacgccagg attttatcct aatctgacag gaacggagaa
    13621 cttggagatt tttgcaaaac tgcgtggtac agccgcccct aattctgtaa aaagagcatt
    13681 ggaaattgta ggcttacctt ataaaaataa aaagctgttt agtaaatatt cacttggcat
    13741 gaaacagcga cttggtattg ccaacgctat cctgcatgac ccagaactgt tagttttaga
    13801 tgagccaacc aatggtcttg accctattgg tattgcggag atgagaaagt ttattaggga
    13861 cttgagcgtt gagcatggaa agacaattct tatttccagt catattcttt cagaaatctc
    13921 attgttggct gatgatattg gaattatcga ccacggtgtt cttttggaag aaagtagtat
    13981 gagtgaacta gaaaagaaaa acagtaaata tatcctgcta caagtttcag atattccgaa
    14041 gacatcactt attttagagc gtcaatttaa cacgaaggat tattccgttc aagatgacta
    14101 cacgttacac ctttataaca ctaatctgga tatgggtgaa atcaataaag cacttgtaat
    14161 gcagaatgta actgttatta gttctcaact ttgtaatgat actttagagg actactttaa
    14221 aaaaatcaca ggaggagagg gcattgctta aacttattca aacagaattt cttaaactgc
    14281 gtcgtaggaa acttatttgg ctgatgttgt tgtctgctct cattatgccg tttttcgcac
    14341 tactatactt caattatttt ggtaaaacag gtgttgagcc aataaagttt tataaatggt
    14401 ctgcatttgg ttatacactt tggattattt tgcctgttgt tttgggtata ttttgcacca
    14461 tgcttataca cgaagaaaac cagtatgata tgctcaaaca gctttggatt gtacctatta
    14521 gtaaaatggg atactttttt agtaaatttt tcgttgtttt gatttactct atttgtttta
    14581 tgctgattac agccgttgct tctgtgttat ttagtgtact ttctggatat gtcgtattta
    14641 gctgggcaag cattttatat ttgttgaaaa aatgtttaga aattggtgta atcactgctt
    14701 ttgtaatgtt gccgattttg gcaatcgcta catctcaaaa aggatacatt ctcccaattt
    14761 gtatcacatt ggtatatgcc ttttccggtt ttgttataat gacaataaat atgtatttac
    14821 acccattatc cagtatggga gttattatca tgagaaataa agacattcca ggtcttatat
    14881 tttcacagga aattaatgtc ccattagcat ttctttgtat ttgtatttgg agtattgtat
    14941 cagtattgtt tgcaaatatt gcactaggaa gaagaaagtg aggtgtagat aatgtggaaa
    15001 ttactttggg ctgaatgtca aaaaattcgt cgttcaaaaa ttgtttggat tacagttttt
    15061 gcatccatta tgatagcagt catagttttt gcatgggggc aggatgtata taatgaccca
    15121 gatttacatt atggcttaaa gagcgtttat gacggttcac ggtatataga caatgcaggt
    15181 tggtatatgg acgcagtaca accttggtca actttttttg ttcttcctgc tgtcattgcc
    15241 ctattaggaa gctatattat ttgtcgtgaa gaagaggagg acactattaa atccctgcgt
    15301 cttattccca taaatgaaac aaatttaacc atagcaaaaa tgattattac ttttatattc
    15361 agtatacttc tttatttgtt actctttact ataacatttt tgactgaatc cgttttacat
    15421 ttttccgatt tatcgacaaa attggtttta agttgcatga aagagtattt tcttgatggc
    15481 ataggtgtat tctttgcaat ttctcccatt attgccttgg tatcgcgcat gaaaaaagga
    15541 tattggcttg cattagtatt cacagaagtt tattctattg ctggactatt tgcgggtatg
    15601 tcaaatgtct tacaaacctt ttttcctatt gcggcaatat ttaatatttc tggatactat
    15661 ataactactg gagaaaaaat tatggggagt atcatgagtt tattactttg tgcctgcctt
    15721 tctgcattta tacttaaagg gctaaaacac aatgaaaaaa attgaaatag gtatatcaaa
    15781 atgaaaaaga taaaaaaact gattattcta attgtattct tgtttctttt tggcatgtta
    15841 cactgttata ctgaatggaa attcaacaat aacccaccta aaggggtaat agaaagaata
    15901 aatgagtgga ctgataagat tggactggat tagtaactta tgttaattat agtgaatgta
    15961 atattagcaa gttattgttt cgtattttaa tactataact ttaaatattt ggttttaatc
    16021 ttccaaacga atataaaaaa actgtcacag aggcaacata tttttgaata tttataaagt
    16081 ggtagttttg attttcaaag ctaccgcctt ttcttctata taagcaaact tatcctcaac
    16141 aaagtgtaaa acatggtgca ttttaagaaa aaagtaccac agacacaagc aataaaagaa
    16201 acgccgagaa cctacaaaaa cgtagtgctt atgcagattt acgacgcctt ctattttctc
    16261 aaccgtctta atgaccttgt acaaaagaaa tttttctaat attttttata atcttatctt
    16321 gtgatgtttt ttatgtataa atgacttaat aacaaatcca ataaaaatta tggatattgg
    16381 tataattaga acgaggaatt tataatcatt ataatgtgac attattctta cccaactctc
    16441 agaaaaaata tatcctatac tgattaatga agtattccat atggttattc ccacgataga
    16501 tgatattaca aaagttataa tattttgacc tgctatacct gctataaaag atatgtatgt
    16561 tctacataaa gggattaatc tacatacaca aacagaaata gaagagtatt tattaaaata
    16621 atcttgagat gcaaatattc ctttctttgc ctttggacat atctctatta ttttatttat
    16681 gatagattta cctccccaat ttcccactgt ataacaaaat atcgaaccaa gtatacctgc
    16741 aattatactt aaaattaaaa ttgtcagaaa tccaaaatga tttcttgcag caattgctcc
    16801 acataaaggt aaaacaactt cgctaggtaa tggaaaacaa gcatactcaa tcataattaa
    16861 tacaaataca gagattaatc catattgtaa aataaaacta ttgattacag acacttactt
    16921 atctatctcc tttcttattt aaaggtttta agggtacatg ttctaaggat tctcacccta
    16981 tatttaaaga atcaatctct ttaaactatt atatgtattt acacaaagca tttataacat
    17041 actttaaaaa tagtacctta aaataagaaa cttaataaat aaaaaaagat tacgtggcta
    17101 cgtcctactc tcccaggcag tttcccacca agtaccatca gcgctagaga gcttaacttc
    17161 tgtgttcgga atgggaacag gtgtatcctc tctgctattg taaccacata atctttatgc
    17221 ttaatatttc
//
LOCUS       DAMKMI010000088        16932 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMKMI010000088 DAMKMI010000000
VERSION     DAMKMI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.090"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..212
                     /locus_tag="PC429_002519"
     CDS             <1..212
                     /locus_tag="PC429_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF3848018.1"
                     /translation="ATVVGWGYNDGKILICDIKDYVPGQTQNLYVIGGAACEKIGSMT
                     KEKFTMIKGNDRFDTLYKALDFINR"
     gene            306..2705
                     /locus_tag="PC429_002520"
     CDS             306..2705
                     /locus_tag="PC429_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3848019.1"
                     /translation="MIFKKYIKSLLIVVSVTLIMNKSILFVNALDLESNNNIKSYKRK
                     NVKEIDISNKFVDESFRRDIVEKVLDNRDDPLTNEVASKDNYKIYESDLNKIESCLEL
                     DLHKNPDVSNPNKIDNLDGIENFRNLEKLDCSSNNLESVDLSKNIKLKYLDISGNKIL
                     NINLKNNNLINELHCDANKLSSLDLKNNKNLVSLFFTSNELTEIDLSNNVNLENLQFL
                     NNNLKSIDLSHNKKLKSLNCTNNKLKSLNLSNNVFLKNLSCSGNNLSKLNIKGLTSLE
                     YLGCAKNKLQDLDVSTNIKLDSLFCGKNSLTKIDISKNKELKSLECSGQYIEKSIIKN
                     GENIVVDIGIENSKLKELYPQNYNISTGFLTWKNISEFPNNVSYSLEYEYGPVNDVET
                     MDVNISIDKLVKCRIRYIDYDGSIINEKEISDNSSINEIQINPQRENYEFNGWRNNKD
                     EKIYTSEQVSKLVFDDSTDFIATYKRKSTGSSGSSGGTSSKEDFNIAPSKEKLTGADR
                     NETSVKISQKGWNKADNIVLINDSSISDALSATPFAKSKDAPILLTKNNNLNKLTEKE
                     INRLEAKNVYIVGGLKSVDEKVVSDLKKKGLNVIRISGNDRYETSIKLAKELDKNSNL
                     SKVVVVNGEKGLADAVSMGAISAKEEMPILLTNQNDDMKDIKDLIANKNISKSYVIGG
                     ESLFNNKEVNNTLPSVTKIAGSDRTETNSKVISHFYSKDTLNDLYVAKNGMNKQDDLV
                     DALSVGVLAGKTESPVVLVGNGLDDSQKELIKNKKFKNITQIGGNGNEKAFNEVENLV
                     K"
     gene            complement(2746..2931)
                     /locus_tag="PC429_002521"
     CDS             complement(2746..2931)
                     /locus_tag="PC429_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDF3848020.1"
                     /translation="MILLEQRKLRVAFNKSGKGSFTPRLILPMSWIKEMNISPDERDV
                     LVTFEDGKIIIEKTENE"
     gene            complement(3479..3655)
                     /locus_tag="PC429_002522"
     CDS             complement(3479..3655)
                     /locus_tag="PC429_002522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848021.1"
                     /translation="MEQKESKIVYSKGGSGTYSAKISLPLSQLEKMGFTREKRKAVVI
                     FKEDEIIIKKSDEE"
     gene            4372..5049
                     /locus_tag="PC429_002523"
     CDS             4372..5049
                     /locus_tag="PC429_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF3848022.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            5223..7259
                     /locus_tag="PC429_002524"
     CDS             5223..7259
                     /locus_tag="PC429_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF3848023.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(7450..8016)
                     /locus_tag="PC429_002525"
     CDS             complement(7450..8016)
                     /locus_tag="PC429_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3848024.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            8304..9239
                     /locus_tag="PC429_002526"
     CDS             8304..9239
                     /locus_tag="PC429_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3848025.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            9704..10918
                     /locus_tag="PC429_002527"
     CDS             9704..10918
                     /locus_tag="PC429_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848026.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETMNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(11338..11859)
                     /locus_tag="PC429_002528"
     CDS             complement(11338..11859)
                     /locus_tag="PC429_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848027.1"
                     /translation="MKFKRLRTSENKLCYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            12215..13012
                     /locus_tag="PC429_002529"
     CDS             12215..13012
                     /locus_tag="PC429_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF3848028.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            13122..13712
                     /locus_tag="PC429_002530"
     CDS             13122..13712
                     /locus_tag="PC429_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF3848029.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            13778..15475
                     /locus_tag="PC429_002531"
     CDS             13778..15475
                     /locus_tag="PC429_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF3848030.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            15689..16528
                     /locus_tag="PC429_002532"
     CDS             15689..16528
                     /locus_tag="PC429_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF3848031.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
BASE COUNT         6762 a         1739 c         2699 g         5732 t
ORIGIN      
        1 ctgcaactgt agttggttgg ggttataatg atgggaaaat actgatatgt gatataaaag
       61 attacgtgcc aggtcagacg caaaatcttt atgtcattgg tggtgcagca tgtgagaaga
      121 ttggttctat gactaaagaa aaatttacta tgataaaggg taatgataga tttgatacac
      181 tttataaggc attagatttt attaatagat agattatata aaatatttta gtatttcttg
      241 aaaatcataa gtatggtaat atttagataa atattattta ttattaatag gtatgggggt
      301 agattatgat atttaagaag tatataaaat cattactgat agttgttagt gttacattga
      361 taatgaacaa atcaatatta tttgtaaatg cattagattt agagagtaac aataacataa
      421 aatcttataa aagaaagaat gtaaaggaaa ttgatataag caataaattt gttgatgaat
      481 cctttagaag agatattgtt gaaaaggtat tagataatag ggatgatcca ttaacaaatg
      541 aagttgcatc aaaagataac tataaaatat atgaaagtga tttaaataaa attgagagtt
      601 gcttagagtt agatttacat aaaaatccag atgtttccaa cccaaacaaa atagataatt
      661 tagatgggat agaaaatttt agaaatctag aaaagcttga ttgtagttca aataatctag
      721 aaagtgtaga tttaagtaaa aatataaaat taaaatatct agatattagt ggaaataaaa
      781 tattaaacat taatttaaaa aacaataatc ttattaatga attacattgt gatgcaaata
      841 aattatcaag tttagacctt aaaaataata aaaatttggt aagtttattt tttactagta
      901 atgaattaac agagatagat ttaagtaata atgtgaactt agaaaatttg caatttctta
      961 ataataattt aaaaagtata gatttaagtc ataataaaaa gttgaaatct ctaaattgta
     1021 ctaacaataa acttaaatct cttaatttaa gtaataatgt ttttttgaaa aatttaagtt
     1081 gttcaggtaa taacctaagt aaattaaata taaaaggact tacaagtctt gagtatttgg
     1141 gatgtgctaa gaataaatta caagatttag atgtatctac aaatataaaa ttagattctt
     1201 tattttgtgg gaaaaatagt ttaacaaaaa tagatataag taaaaataaa gaattaaaat
     1261 cattggaatg tagtggtcaa tatatagaaa aaagtattat aaaaaatggt gaaaatatag
     1321 ttgtagatat aggaattgaa aattcgaaat taaaggaatt atatccacaa aattataata
     1381 tctcaacagg ttttctaaca tggaaaaata tttctgaatt tccaaataac gtaagttata
     1441 gtttggaata tgaatatggt ccagtaaatg atgtagagac tatggatgta aatatatcaa
     1501 tagataaatt agttaaatgt agaatcagat atatagatta tgatggaagt ataatcaatg
     1561 aaaaagaaat atcagataat tctagtataa atgaaattca aattaatcct caaagagaaa
     1621 actatgagtt taatggttgg agaaacaata aagatgaaaa aatatataca tcagagcagg
     1681 taagtaaatt agtttttgat gatagtactg attttatagc aacttataaa agaaaatcaa
     1741 caggatcaag tggttcttct ggtggaactt caagtaaaga agattttaat atagcaccat
     1801 ctaaggaaaa attaacaggt gcagacagaa atgaaacatc agtgaaaata agtcaaaaag
     1861 gatggaataa agctgataat atagtgttaa taaatgactc tagcatatcg gatgctttat
     1921 cagcaacacc atttgctaag tctaaagatg caccaatatt acttactaaa aataataact
     1981 taaataaatt aacagaaaaa gaaataaata gattagaagc aaaaaatgtg tacatagtgg
     2041 gtggattaaa gtctgttgat gaaaaagtag tgtctgattt aaagaaaaaa ggattaaatg
     2101 taattagaat atctggaaat gatagatatg aaacatcaat aaaacttgca aaagaattag
     2161 acaaaaattc taacctgtca aaagtagtag tggtaaatgg agaaaaggga ctagctgatg
     2221 cagtaagtat gggagctata tcagcaaaag aggaaatgcc aattcttctt actaatcaaa
     2281 atgatgatat gaaagatata aaagacttaa tagctaataa aaacatatca aaatcatatg
     2341 taataggtgg agaatcttta tttaataata aagaagtgaa taacacatta ccatctgtaa
     2401 ctaaaatagc aggttctgat agaacagaaa ctaactcaaa agtaataagt catttctata
     2461 gcaaagatac acttaatgac ttatatgtag ctaagaatgg aatgaataaa caagacgatt
     2521 tagtagatgc cttatcagta ggagtgcttg caggtaaaac tgaatctcca gtagtattag
     2581 ttggaaatgg attagatgat agccaaaaag agttaatcaa gaataaaaag tttaaaaata
     2641 taactcaaat tggtggaaat ggaaatgaaa aagcatttaa tgaagtagaa aacttagtta
     2701 aatagattga aaggtagtaa ctggaaatag ttactacctc cttttttatt cattttcagt
     2761 tttttcaata attatttttc catcctcaaa agtaacaaga acatcccttt catcaggaga
     2821 gatattcatc tctttaatcc atgacattgg aagtatcaat ctgggtgtaa aagagccttt
     2881 gccactttta ttgaatgcaa ctctaagttt tctttgttcc aataatatca ttcctttcta
     2941 ctattaataa atgccagcaa taataaagta aataccaaag caataagagc tttaaaagta
     3001 aaacttatta ttatattaat aatatttaaa actatagtga taccaagaat aatcatacaa
     3061 attctataaa atttattatt catattaaat tagaaaatgt gttataatat tagaaagaag
     3121 ctacctagaa ggaaggtatt ttcctcctag gattgaaact tataattctt taatcaatct
     3181 tagtattgcg agtactagat taattaaaac taatgtgaaa gagaaagcat ttttaatttt
     3241 attaaatttg ttttctcttt tattttttct tctttctaat ctccccactt tctttgtcct
     3301 cccttctaag ttttttattt tcttacctcc tttctatatt tatattatat catcacgtga
     3361 cgtgaaagtc aataaatatt ccatattttt caataaagct ataaaatata ttaatataaa
     3421 atacaaaata taataaaaat aagtggatgt ttacattata cacccactta ttttagagtt
     3481 attcctcatc agattttttt attataatct catcttcctt aaaaataact acagccttac
     3541 gtttttccct tgtaaatccc attttttcaa gttggcttaa aggtaaagaa attttagccg
     3601 aataagtacc tgaaccacct ttactgtaaa ctattttaga ttctttttgc tccaaactat
     3661 tttcctcctt ttttatattg taatgtaaca aatattaaca ataaaatgct tactattaat
     3721 cctattatat tgttgatatt aaaatgtaaa aataaaagta ttatgtctat tactatagtt
     3781 atagcaacta taataagaca gaatttataa actttatcac tcatatttta tagaaaatgt
     3841 gttataatat ttagaaagaa gtcactagga agaagattaa attcttccta gcttacaaaa
     3901 ctataaatct tttaataatt taattagttt atcaattaaa tttattaaaa tgggtaagat
     3961 agagagagca gttttaactt tggcgagttt gctttctcta tctttttttc ttctttctat
     4021 tctactcatt ttccttttca ccccctttct atatttatat tataatatgc cgaaccggca
     4081 aaagcgatat ttatttccat atttttcaat aaagttatat tgacaaacta tattttttta
     4141 caaataaaaa taagggtggc aaaaccaaac ttgccaccct tttgccaccg aaaataaata
     4201 atagaataaa tttaaattaa acataataaa acataaacaa caaaactgac cttaataaaa
     4261 cattgaaata ccaacacttg taaaaaataa caacaataaa caaataacct agaataatat
     4321 tacaacttaa gtaaataata attttgaata ataatttacg gaggtattca tatgtttaaa
     4381 cacgataaag cattattaaa agaagtaaaa gtagaaagac caaatcctca atatgctgtt
     4441 ttaatgcaag aacagttggg tggtgcaaat ggagagttaa aagcagctat gcaatacttg
     4501 tcccaaagtt ttagaataaa agaccctcaa ataaaagatt tatttcttga tatagcagca
     4561 gaagagctta gccatatgga aatggtagca caaactataa atttattaaa tggacatgat
     4621 gttgattata attcagttaa tacaggcgaa atagaaacac atgtattaac tggtctatca
     4681 ccagtcttaa taaactcatc aggagctcct tggactgcta attacgttac agttacaggg
     4741 gatttagtag ctgacttact ttcaaatata gcttctgaac aaagagctaa ggtagtttat
     4801 gagtacttgt atagacaaat agatgataaa tatgtaaaag aaacaataga tttcttactt
     4861 aatagagaag aagcacataa tgctctattt agagatgctt taaataaagt taaagataca
     4921 ggttcaaata gagattttgg agttacagaa gattctaaat tatatttcga tttgtctact
     4981 ccagggccaa atcacgatac taagatagat attaatcctc cttcttttga aaaaccttta
     5041 aaaaaataat ttgatatttt ttatgttgtt ttgttttagt agttataaaa attatttact
     5101 aggagaatta attatatgaa aaattaaata tgtatggtta aaaatgggta tacacttatt
     5161 ataagtgtat atctatttgt ttattgcaaa acatataata aaataacaaa tgaggtggta
     5221 ttttggacag taatagaaaa cctaataatc taataaatga aaaatctcca tatcttttac
     5281 agcatgcata taatcctata aactggtata gttggaatga tgaggctttt aaaaaagcaa
     5341 aagaagagga taagccaata tttttaagtg taggatattc aacatgtcat tggtgccatg
     5401 ttatggaaaa agaatctttt gaagatgaag aagttgctga aataatgaat agaaactttg
     5461 tggcgataaa agtagataaa gaggaaagac ctgatgtaga tagcgtgtat atgacagttt
     5521 gccaagccat gacaggtagt ggtggatggc caatgaccat aataatgaca ccagataaaa
     5581 aacctttttt tgcgggaaca tatttcccaa aatattcaag gtataataga cctggagtta
     5641 tagatttatt agagaatgta tctgaaaagt ggaatacaag cagggatata ttaataaaat
     5701 ctggagatga aataatagaa gctcttaagg atgattttgg tgtaaaaaat actgagggtg
     5761 atttatcaaa agagatgttg agttcatcag ttagagtatt taaggctatt tatgatgaaa
     5821 agtatggtgg ttttggtaat gctcctaaat ttccaagtcc tcagaatctt atgtttttaa
     5881 tgaaatatta tagtatagaa aaagacaaag atgtgttaaa aatggtggaa aaaactttag
     5941 atggaatgta tagaggaggc ttgtttgatc atattggatt tgggttttca agatactcta
     6001 ctgataaaaa gtggttggct cctcattttg aaaaaatgct atatgataat gctatgctta
     6061 ctatagcttt tttagatgcg tataaaataa caaagaaaga gttatataaa gaaattgcta
     6121 taaaaactat agattatgta gttagagaga tgaaggataa agaaggtggg ttttattcag
     6181 cacaggatgc tgatagtgaa ggtgaagaag gtaaatttta tacatttaat cctcttgaga
     6241 taattgaagt tttgggtgaa gaagatggaa ttttctttaa taattatttt gatataacta
     6301 gtagtggaaa ttttgaaggc aaaagtattc ctaatcttat aaaaaataaa gaatatgaga
     6361 gacataatga aaagatagct gaccttagta aaaaggtatt tgaatataga aaagaaagaa
     6421 catctttgca taaagatgac aagatactta catcatggaa tgcattgatg atagttgctt
     6481 taacaaaggc atatagtact ttaaaaaatg acatatattt agagtattca aataagtgtc
     6541 ttaactttat aaataataat cttgtaaatg agtcaggtag attattagct agatatagag
     6601 atggaagttc cgactattta gcatatttag atgattatgc ttttttaatc tgggcgtata
     6661 tagagcttta tgagtcaaca tttaatatga aatatttaga aaaggcttta aatttgaatg
     6721 aaagctgtat caaccttttt tgggattatg aaaaaagtgg attttatata tatggtaaag
     6781 atagtgaaaa tttaatagca agaccaaaag atttgtatga tggagctatt ccatcaggaa
     6841 attctgttca attatacaat cttataagac ttgcgaaaat tactggagat aatagacttg
     6901 aagaaatgtc atataaacaa ttaaaactgt atgtggataa tgtaaagagt tctccaactg
     6961 gctacagctt ttatatgctt tcattgatgt ttgaacttta ttctactaag gaaattatat
     7021 gtatttttaa ggaagattct gatttaattg catttaaaga attgataagt gaaaatttta
     7081 taccaaatgc aactttttta gcaaagaaat acaatgaaga aaacactatt ataggttttt
     7141 taaataacta tatattgaag gatgataaga ctagttatta cgtgtgtcaa agtaatagtt
     7201 gtagtcaacc aattaatgat ttacaaaaat taaaagatat gattttagga gtagaataat
     7261 cataatagcg aaaaaggaga tgctttaaaa taaaagtcta atcatctcct ttttttgtat
     7321 aaaaaacaga aaaagagttc ctaagaacac ctaatctatg taatgattgt tttgttaaaa
     7381 aataaaaatg acattatagt tatataaaat attgtactat aaaaaagtgg tattatagat
     7441 gcgatagtat taatcttttt tatttaccct atttcttctt tttgcaaatt cttcaagtgc
     7501 ttcagattta attttatcta tttcttcata ttccataaaa ttagcatctt cttcaaagcc
     7561 atcttgttct tcaccattaa aacgatacat tttttttagt aaactccata aggtctctaa
     7621 ttcatctttg gtaaattcgt gaaatacatc tgccataaag tttattccag tcctactaca
     7681 tgttaccata accttttttc ctaagtctgt aactttaaca ttaattgctc tcttatcatg
     7741 aggacttgga attacatcaa catatccatt tttttcaaga ttagcaacta gtctatttat
     7801 attttgttta cttgtaccca tttttcttgc aatattattt aatgttgttt cttcctctgg
     7861 taaatgaaga attgataaaa tagtcatata ctgtcttgaa gtaagtatac caaaatattt
     7921 atctccttct atttgtattt tgtttgtaag ggtaaaaaga gttgcatatg tttgattcat
     7981 taaaaagagc tcttttagtt catttgaata atccatgaaa tatatctcct ttatttttag
     8041 atgttgtttt gtcgaaaaca gaatatcatg aattaattct aatgtcaata tggtgtcaaa
     8101 atatattaaa gtattacttt tttatgtaac tattaattaa ttaaattatt gtataatttt
     8161 atgtaaaaac ttctatgtac caatgataat ttctatgagg aattaataat tcttaaattt
     8221 tttagaaaga ttttgacttg aaaatacttg taatataata tataaattaa tatactgaat
     8281 aaaatcaatc tggagggata attatgaagt gtacaaactt tactttcaaa ggagaggaag
     8341 gtttagatat atatacatat aaatgggaag atgaaaatat aaaaaaacca aaggcagtta
     8401 tccaaattgc acatggaatg gctgaaactg cacaaaggta tgagactttt gccaaggtac
     8461 ttactaaaaa tggttatata gtatatatca atgaccatag aggtcatgga aaaactgcaa
     8521 agataataga aaatgttgga catttagctg aaaaagaagg atttagatgt cttgtagagg
     8581 acatgtatac tttaacaaat attataaaga aagaaaatga agatttgcca atttatctat
     8641 ttggacacag catgggttca tttgcaagtc aaagatatat aatggattac agtaataatt
     8701 tatctggtct tatactatgt ggttcaaatg gaaagcaagg aatcattcta aatcttgctc
     8761 atctgataat caatcgtgag attaaaaagt atggaagacg ttctaaaagt aataaaataa
     8821 ataatttgat atttggtggc gaaataataa gaagaaatga aaagactaag tttgactggt
     8881 taagtagaga taaagaacaa gttgaaaaat atataaatga cccattttgt ggagtagtgt
     8941 gtagttgtgg ctttttttat gatttagttc aagggttaaa agaaattgag gataaagaaa
     9001 acttaaaaaa agtgccactt gatataccta tttacataat atctggagat aaagacccca
     9061 taggaaaaaa tggtaaagga gtcttaagac taagggatag atatataaaa ttaggagtaa
     9121 aagatgtaac ttgcaaactt tacaaagatg gaaggcatga attgttgaat gaaataaata
     9181 gagaagaagt ttttgaggat ataatttgtt ggttaaataa taaaatagaa atattgtaat
     9241 aaaaaaatat aatgatatat ttgctttaaa tttatttata tataatattt gaaaagtgga
     9301 ttatgtatat gcaaagtaga aaagagagag cctagaagtg attttttgct ataatgccat
     9361 ttattgaata ggtgggtgtt attataaata aattattaac actcagtatg attatcattg
     9421 tatatggaat ttattttatt taaaattcct tgaaagttta aatatagtaa gttgtaataa
     9481 agagaaaatc taaatattta aatgctattt aatgtgaatc tattaattag gttatactta
     9541 aacttaatta atagatttta tatttcaagc caatgagata gggttttaga ttaaaatata
     9601 accaatggtt aaattacaga cattttgtta actttccctc gatttactag taaatatttt
     9661 ttttatatac ttaaattaca acagatgtac tagggaggta tatatgcaaa ttaaaatagg
     9721 taaagtcata cagtgtctta gaaaagaacg aaatttaact caagaacaat tagcaaagtt
     9781 tataggtgtg tcaacaccag cagtatctaa gtgggagagt ggaaattctt acccagatat
     9841 agaattattg cctttattag cagacttttt taatgtgtct attgataagc ttttaaatta
     9901 caagattgat ttaagtgaag aagaagtaat gaaaatttat aaagaattag aatcaggctt
     9961 tgctagaatt gaaattgatt tatcaacaga agaaccaaaa gaagaattta gacaagactt
    10021 agaatctgta aaaaagcttt ccaatatgta tatagagaag tatcctaaaa gctatttatt
    10081 gaagctaagg atatgttcgc tgtatcagat gtattcatat aaatttggta agagtgaact
    10141 taatgacaga gtaaaagaaa caatgaatat tcttgaagat atagtgagaa atacagatga
    10201 tatccaaata aaagaaacag ctcttattat attatcaaac gcatattgta tgttggagga
    10261 ttatgaaaag gcagagttgt atttaaatat gatacataag tcaatagggg atacaagtgt
    10321 taatttagct atgatatatt taaagcaaaa tagacttgaa gaagcagaaa tattgcttca
    10381 aaataaattg tttagtaatg tatttaatat aagcatggat tgtaagggaa taattaatgt
    10441 atacaaaaat caatacaagg aattaaagaa aaaattggag aatagaaatt ttaataaaaa
    10501 tgcaattgag agagaaatgg agtatataaa aaataagttg ctggggtatg caaatctttc
    10561 tttagaaata aaaaaaatgc tcagtgaaga taaaggtgca ttttttagta tgtatatgga
    10621 ttatatggaa ttatctctaa tttttttatt ttttaacatg aaagaagaag caaaaaaagc
    10681 cttatatagt ctaaaagaaa tattagaaaa atatcctata catgaaagtt tggatgtaag
    10741 tcaaatgaga ttttttgata aggtagagtc taaaaattta tatactttta atatatatac
    10801 aaacttgtta attgtactta atgatgatag ttataatgag cttagagaag aaccaatttt
    10861 taaggatgta attgaaaaga ttttagatat ggaaaaaatg ttgaaaaata aagaataagt
    10921 aaaaatttaa tataggactt aaagattaat caaattaaaa gaaagtgttt caaaataaaa
    10981 atttgaatca ccttttttgt cggtcttaaa cagttattta gaaaaataga ggtttcaaaa
    11041 tagaaatctg aattatctgt attattacac aaaaatagaa acctaaattt ataaaaatag
    11101 aataaagttt gttatataaa atgtaatttt gtctataaaa tctcacttgt tttcttaact
    11161 ttaagcgtga aaaagagaat gtatcatgaa ttttttagtt catgatgcat ccttctttgt
    11221 atgaaatcca atatatcttt attatttaaa caatggaaaa gagccttatt tctattttga
    11281 gacatctctt ttatttaatt atgaagtttt atacagttca ataaatagga attatattta
    11341 gaaactcatg acattatttc ttagatagtg attaaattta tcatattcca ttttagataa
    11401 cttttctggg taagacatta caatcaggtt atgcccatta aatttttcat ggtatggtgg
    11461 atgaatgtgt tcataattat ttgtcttata ttttactctt tcatagaacg ccttgcgata
    11521 aattgaaatc tcatcaattg gaaggtcaat ttctaaaata atagtttttc ctttcttatt
    11581 taaaagctct aacgctcttt gtccatatct attattgcgc atttctggaa aaatgcagaa
    11641 atgttctaca tagataaagt catctgtttc ccaacataaa attatgccga caaactggtt
    11701 tttatcataa attaaattga actgatattc tttatttttc aaaatttctt cttgcaaaga
    11761 agattttctt tgttcatgaa atgggaaact aattttatat aattccatag ccttgtaaca
    11821 taatttattt tcactggttc ttaaccgttt aaatttcact ttactcctcc taatcacgaa
    11881 aattccaatt tatttatgag ttcattttac catagtcagt atttgcatac aatgtgtttt
    11941 aagttgagtg tcatttcttt cttttattta tgcttatatc tcatagacta agagataatt
    12001 gcttaagaca taactaaaaa ctattatatt aagctttgat aatctaaaaa cagtatattt
    12061 atggactttt ctaggtctaa aagtaatctt aataatttta gaagttacaa aaaagttaca
    12121 taaatttgga aagctataat atacatatat aatataaggt taaaactgta atgaataata
    12181 ttaatttaaa atttgaacat ggaaggagat tattatggag aaaagagaaa agaggataat
    12241 aatatcatct ttattaagtg tatcaatttt aatgggtttg gtaagtatat attctatatt
    12301 aaataaagaa gacataattt taactgttaa gggtcaagag caaaaagtat cttcctttaa
    12361 gaaaacagtt gaggaacttt tggatgaaca gggtataaag tataattctg aagataagat
    12421 taatccaagt ttagatacag aactaaaaga tgatatgaaa ataaaagttg ttaaggtaac
    12481 taaaagtaaa aaagaagaga ttgaaaaaat tccatttgat acaaagcatg taaatgatag
    12541 taatttgtta aaaggaaaat ctaaagttta tcaagaaggt caagaaggag aaaaaaaact
    12601 agtctataat ttaacttacc atgatggaaa gttagtcaaa aaagtcttat caaaagaagt
    12661 aatatctaag gagccaacta caaaaattat aaaatatgga actaaagaaa aagtactaat
    12721 agcatcaaga ggagcaaata taagaggagg caaacatatg aaagtggttg ccactgcata
    12781 tgcaggagat acaataacat ctactggtac aactccaaga tggggtgtta ttgcagttga
    12841 cccacgtgta ataccatatg gaacaaaagt atatatacct aaactgggta tgacttttgt
    12901 agcagaagat tgtggaggtg caattaaagg taatagaata gacattttta tgaacagtga
    12961 aggaaaagct tcaaattggg gaaggaaaag tatagatata tatctacatt aaattagtga
    13021 atagaataac tttacttgaa ggacaaaaaa gtataaaata tgaattaact aaactgttag
    13081 attattctta aaacataaaa atatatctaa ggggggataa gatgaaaata gtatgcttag
    13141 gagacagttt aacgtatgga tttggagtat cacgaagtaa ttcatggaca aacattgtaa
    13201 ataaagagac tcgattagaa atagtaaata agggtataaa tggtgataca acaagtggta
    13261 tgttagctag gtttaatgaa gatgttgtaa aaaattctcc tgatatagtt tttataatgg
    13321 gtggcaccaa tgattttatt gctggtgcgg gaaatgaaat tattaattct aatataatgg
    13381 ctatggtgca tcagtcatat tcaaaaaata ttatacctat aataggtata ccattaaaac
    13441 cagatattcc aaatgttagg gaagattgga gttgttttac tgattttaat atagtatctc
    13501 aaaacttaga atcatatagt tattggataa aaaaattttg tatgacattt aatactaatt
    13561 tcgtagattt ttattcagaa tataataaaa atatggaact agaaggatat aaaaaattat
    13621 attttgatgg gttacatcca accaaagagg gtcatagaat aatggcagac atttttatta
    13681 attcaattaa taagtatata gaagagttat agattacatt aaaatgcttg tttatgcgtc
    13741 agttgcaata aatacataca gtagagaggg gtttattatg gattatacta aaacttatga
    13801 agagtggata aagggttcat attttgatga agatacaaag ctagagttgg agaatataaa
    13861 aaataatgaa aaagaaatag aggatagatt ctataaggat ttagaatttg ggactgcggg
    13921 tcttagagga ataattgaag caggaacgaa tagaataaat aagtacacag ttagaagagc
    13981 tacttttgga ttggcaaact atatattaga aaacacaaca aaagaagaaa caagtagagg
    14041 agtagtaata gctcatgata atagacacaa gtctagacaa ttctgtatag aatctgcaaa
    14101 tactttggca gcatgtggta taaaagcata tatatttgat agtttgagaa ctactccaga
    14161 attatctttt gctgttagaa gcttaaatgc aatcgctggt atagtcataa cagcaagcca
    14221 taatcctcca gaatataatg gatataaggt atactgggaa gatggagcac aagttatgcc
    14281 agagattgca aatgcaataa cagagaaagt aaatagtata catgattata gtacaatacc
    14341 aaccttaacg gaagctaata aaaatttggt agttttgttg gacgaatcac aagatactaa
    14401 atttatagaa gcagtgaaaa gccaaattat aagaaaagat ttagttaaaa atgtaggtaa
    14461 aagctttaag atagtttata cacctctttg tggaacaggg aatgttccaa taagaagagc
    14521 attgaaagaa gttggttttg aaaatataat tgtagttcca gaagaagaaa atccagaccc
    14581 aaattttgca gggcttgatt atccaaatcc agaagagaaa aaagctttaa atagaggtat
    14641 attactcgca aaagaaaaag gagcagacct tgtaatagca actgacccag attgtgatag
    14701 agtaggtgta gcagtaaaaa ctactacagg tgagtatgct ttacttacag gaaatcaaat
    14761 tggtggtatg ctgacacatt atataataga aggattaaaa gaaaataata aattaaaaga
    14821 aaatcctaca ataataaaaa ccatagttac atcagaattt ggagcagata tagctaaagc
    14881 aaataatgtt gatgtattaa atgttcttac aggttttaaa tttattggtg aaaaaataaa
    14941 attatttgaa caaaataaaa atagaagtta tgtatttgga tatgaagaaa gttatggtta
    15001 cttagttggg actcatgcta gagataaaga tggtgtagtt tcttcattgt taatatctga
    15061 aatggcagca ttttactatt caaaaggtat gagtttatat gatggtttaa tagaacttta
    15121 taaaaagtat ggatttttca aagaacagac tatatctttg actttaaaag gtatagaagg
    15181 tgtagaaaag ataaaagaaa taatatctta ttttagagaa aatcaaattg attatataaa
    15241 tagtattaaa gtagttgata aaaaagatta taaaaatggt atagataatc ttcctaaatc
    15301 tgatgtttta aaatactttt tggaagatga atcttgggta gctataagac catctggaac
    15361 agaaccaaaa cttaaatttt acatagctgt aaaaggagct agtgatattg aagcagataa
    15421 aaagattcaa ggtttgaaaa agtatataga tgacatggtc gaaaaattaa aataaaatat
    15481 tattatttta taagataatt ttgtaataat atttatttag ctgaaaatta taatttactt
    15541 tcattgagta gaatatggaa atgttagtag aattgttatt ttcagctttt atttgtgtta
    15601 tttttactta aataattata ttatagaatg aatctgagta taattaatat tgtataatga
    15661 aattaatttg atagaaaagg ggatatttat gtttggtata gataagaaat tgattgaata
    15721 tataattgca aaatccaatt acagtgaatt aagttcaaag tcagcagaag tatctttctt
    15781 cttaatgttg tccatatttc cttttttaat atttacaata agttctatag cttatatacc
    15841 gattcttcat ttaaataagt atatagcttt gtttagaaat atgatgccag aaggtgcttt
    15901 tgctgtgtta tcttcaatca tagtttctgc aatagacaat agaaacctga aatttttagc
    15961 agtcagtttt gtacttacta tgtggacatt ttcaagagca gtaaaagctc ttattaaagg
    16021 aatgaataga gcctataaag ttaaagaaac taggtctttt tttaaaattt tatctatatc
    16081 atttttattt acaataatgc ttttggtcct tattttttta tctatgatat ttttagttta
    16141 cggtgaaaaa ataggttatt ttatttttaa tcttgtagga ttggatgaga tatttataaa
    16201 aatatgggat attctaagat acactgtagg tataataaca ataatagtga tatttacact
    16261 tttatataaa tacactccaa ataaaaagtt aactattaaa gaatctgcgc caggtgctat
    16321 ttttgctact tttgcttggt ttttagtatc atttttatat tcttattata caaattacta
    16381 tgctaactat gaagtaatct atggaagtat agctgaaata attgtactta tgacctggat
    16441 gtattttagt agttggtcta tcgttattgg ctatgaggta aattcaagat tgtattttag
    16501 aaagataaga catgaaatgt taaagtaaag tatttaaaat aaaagagagt atttagtatt
    16561 ataaatgtaa ttgaattaat aaagaaatat tgacatttaa gttaaaaagt ctagaatttt
    16621 tctgggcttt ttttattttt agtataactt gacaaatttt atttaatggg atagttttaa
    16681 tttataatca tctttaaaaa gatgactatt gataagtaag agataaaatt ggtaagtatc
    16741 aaaaaatata ctaaataaaa attatagtcc taatgagtta atttctgtat tttcaaataa
    16801 tgcagtattt agtcatgtgt ttttatatgc gcctgtattt ctaattagca atatattaga
    16861 ataagctact gtaattagat ttagaaaaaa gcgacataaa taattataaa caaataataa
    16921 attacataaa at
//
LOCUS       DAMKMI010000089        16741 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMKMI010000089 DAMKMI010000000
VERSION     DAMKMI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.091"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            161..895
                     /locus_tag="PC429_002533"
     CDS             161..895
                     /locus_tag="PC429_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3848032.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGSPIEYTISIFRTDKYQYEIVLSE"
     gene            905..2038
                     /gene="nagA"
                     /locus_tag="PC429_002534"
     CDS             905..2038
                     /gene="nagA"
                     /locus_tag="PC429_002534"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF3848033.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGDVVYKNI"
     gene            2137..2886
                     /gene="nagB"
                     /locus_tag="PC429_002535"
     CDS             2137..2886
                     /gene="nagB"
                     /locus_tag="PC429_002535"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF3848034.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            3061..3333
                     /locus_tag="PC429_002536"
     CDS             3061..3333
                     /locus_tag="PC429_002536"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848035.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            3993..4697
                     /locus_tag="PC429_002537"
     CDS             3993..4697
                     /locus_tag="PC429_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848036.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKIWGYDIEVETRAADDTVKRLRKKILNTNI
                     LIETVWGFGFRLKEKS"
     gene            4699..6186
                     /locus_tag="PC429_002538"
     CDS             4699..6186
                     /locus_tag="PC429_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3848037.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            6391..8004
                     /locus_tag="PC429_002539"
     CDS             6391..8004
                     /locus_tag="PC429_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3848038.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LCFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(8367..9197)
                     /locus_tag="PC429_002540"
     CDS             complement(8367..9197)
                     /locus_tag="PC429_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3848039.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKSACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            9270..10997
                     /locus_tag="PC429_002541"
     CDS             9270..10997
                     /locus_tag="PC429_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848040.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSRFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FIYDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            11034..12896
                     /locus_tag="PC429_002542"
     CDS             11034..12896
                     /locus_tag="PC429_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848041.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            13229..13918
                     /locus_tag="PC429_002543"
     CDS             13229..13918
                     /locus_tag="PC429_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF3848042.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYPIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            14014..14700
                     /locus_tag="PC429_002544"
     CDS             14014..14700
                     /locus_tag="PC429_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF3848043.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            14717..16624
                     /locus_tag="PC429_002545"
     CDS             14717..16624
                     /locus_tag="PC429_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF3848044.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKHSGNQSDEKEVNAELTSSVVKA
                     NTDNENKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPREAGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
BASE COUNT         6591 a         1736 c         2884 g         5530 t
ORIGIN      
        1 ctagcttctt atatagtata aactccaatt tcttttttgt ctttaggttt tattaaaatt
       61 tttacaaaat aagttaaaaa gtattgaaat aaaatttctt ttaaagtata attggtatat
      121 accaaaattg gtatatacca tatcgaaaag tggtgactat atgaaaatag taaataaatc
      181 tagtaatata ccattgcata ctcaattaag ttcaataata agagagatga tagaaactgg
      241 tgagcttaaa gagggtgatg caataatgcc agaaagagaa ctttgcaaca tacaaaatgt
      301 aagtcgtatg acagtaaata aaaccattgt tggattagtt acagaaggac ttctttatag
      361 ggtacaaggt aaagggacat tcgttgcaaa gcaaaaaaag aagtatcagt tttcaaatgt
      421 aaaaggtttt actgatgtta tgaaagaaaa gggagtaaac ataaaaacgg atatattatc
      481 ctttgagatg gaactaccag atgatttagt taaaagaaag cttggaataa gtgataatac
      541 tacaaatata tataagattg tcagacttag atatactgat ggagagccat ttggattaga
      601 aatagtatat ctatcagaag aaatgtgtaa gggtctaaca aaagggatat tggataattc
      661 ttcattatat agggttttaa atgaaaaata tggctataag atacaaaaag ctgaacaggt
      721 aatggagcca gtaatacttt cagatgaaga aagtaagctt cttgaaacag atgaaggtgc
      781 tttagctctt aaacttcata gaaattccta taatagagaa ggtagcccta tagaatatac
      841 catatcaata tttagaacag ataaatatca atatgaaata gtattgagtg agtaggtgaa
      901 taatatgaaa tgtttaatta atggaaagat aatattaaaa aatcaaatct tagaaaataa
      961 agtattagta tttgatgaaa aaataattga tatagctgat agtgtaccaa aagattgtga
     1021 agttatagat gctgatggaa aatatatatc tccagggctt attgatattc atatacatgg
     1081 aaacatggga aaagatacaa tggattcgac tgatgaatct attgaaacaa tttcaaagtc
     1141 aattatgaga catggagtaa catcttttct tccaactaca atgactatgg ataaagaaca
     1201 tgtttatgat gcacttgaag ttattaaaaa agcccaaaat agaaagcttg aaggggcaca
     1261 agtattagga gcacatttag aaggaccttt tataaatgag aattataagg gagcacaaaa
     1321 tgaaaaattt ataataaatt caaaatacga atttataaaa gaatataaag atgttataaa
     1381 agttataact tatgctccag aaaaagatat tgattttgat tttactagag aaataaaaag
     1441 atgtacagat atagttttat ctattggtca tagtaatgca aattatgacc aagctaaaga
     1501 agctataaat ttaggagtaa ctaatgtaac tcacatgttc aatgcaatga ctggtttaaa
     1561 ccatagagac ccaggcgttg ttggtgcagc acttacaact aatgtgtaca gtgagttaat
     1621 agcagataca atccatataa ataaagattt atttcaattt atactaaata ataaaggaaa
     1681 agaaagactg attttaataa cagattctat agaagctggt ggtcttgaag atggcaatta
     1741 ttctcttgga ggtcagaaag tcatagttaa gggtaatgaa gctagacttg aaaatggagc
     1801 attagctgga agtgtgttgt ctttaaataa aatggtattt aacttcttag ataatacaaa
     1861 ccttaaagtg aatgaagcaa ttaatttagc ttctttaaat ccagcaacat ctttgggaat
     1921 aaatgacaaa aaaggaagct tagaaatagg taaggatgca gatatagctg tatttgatga
     1981 gaatttagat tgtaaaatga cactttgctt gggggacgta gtatataaaa atatatagta
     2041 tacaaaacaa accttaaaga cagtaaaaac aatataaaaa ataataaatt aaacttacat
     2101 tagtaaaagt agtagttaaa atttaggagg aataagatga gagtattagt gtgtaaagat
     2161 tatgatggaa tgagtaaaaa agccgctgaa atgatagcag ctcaaatagt tttaaaacca
     2221 aattcaatac tgggactagc tacaggaagt acaccagtag gtatgtatag agatttagta
     2281 aaaaaataca atgataatat agtagatttt tcagatgtga tgagctttaa tttagatgaa
     2341 tattataagt taccaatatc taatgaccaa agctatgatt attttatgaa agagaatcta
     2401 tttaatcatg taaacataaa gccagaaaat actcatctac caaatggaat ggctgatgat
     2461 attgaaaaag agtgtatgaa ctatgaagct tctatagatg cagctggtgg tatagatgta
     2521 caagtacttg gaataggtcg taatgctcat ataggtttta atgaacctga tactaaattt
     2581 gcaaagagaa ctcatgtagt tgaacttaca gagtctacaa tagaagctaa tgcaagattc
     2641 tttaaatcaa gagaagatgt tcctaaaaaa gctgtaagta tgggaatagg ttcaatattg
     2701 aagtctaaga aaatattgct tttagcttca ggagaagaaa aagctgatgc agtatataat
     2761 actgtttatg gagatataac tccagaagtt cctggttcta tacttcaact tcataaagac
     2821 actatagtta tagtagatga agcagcagca agtaaattga atccaaaaga ttataaatta
     2881 gtataagaat agaatgtagt ttaagtaaaa aatagctatc attgagtaat caaatttatt
     2941 taatgatagc tatttttatt taaatttttt acttcttttt atttaaatta gttattattg
     3001 tttaaattac ttgcttatta ctttttacga aaactcgtta tcaagaagta tccaaataag
     3061 atgttaaaaa ttattatgtt tagtgggatt ctatcaagca taataaaatt acctataatt
     3121 gggatattac caactggaaa cataaagaag aatacaggca tcagtgatag taaaaaagat
     3181 ggaaaacaaa gaagaagtaa tcttggaata tcaatatacc aactaccatc ctttttgtat
     3241 tctctaatca ataattcagt tcctagtaat aaaccaatac caccagagga aattaaatta
     3301 ataaaaatgt atcttttaaa gtcttgcata taaccagtgt aaaattgagt atagaaatca
     3361 taatttaatt taacggctaa aaaatacata agcaatagaa tgattaaaaa taaaagatat
     3421 ctaagagctt ttttcatttt aaattctccc ttcagaaata ctttatttag ttttattttg
     3481 ttggaatctg attttatcat ttagtttaag aaatccataa tattagtgaa gaatgaaata
     3541 agtattatat atgaataaat atttttagaa ttaaggtgaa ttgtagtgct taatataact
     3601 ataatattaa ttgtttgaat attcaataaa aagtttgaaa aatatcaatt ctgatataaa
     3661 ctgaataaat acagttgaat attaaattat tattttatca aaaatatgca aagtactaca
     3721 ccttcaaata tcacctaaag taattaaaag gcaattaagg tgaagtagtt caagtatttc
     3781 taaaataagt ttaataaatt ctataataat ttattttttg ccttaaaatt gccttaaaat
     3841 ccaaacaatt ttaaaactaa tgtactataa aaagctggta taatttaaat atagtaaaaa
     3901 ataagattta tcttatctat tgggatttaa ttatttaatc aatatattta taccatttaa
     3961 tttaaataaa aagtctatta aggagtgagc ttatgagtaa gttaatttat atagcagatg
     4021 atgaagataa tataagaaat ttggtaaaga cattcttaaa aaatgaaggt catgatgtta
     4081 tggattttaa aacgggtgat gaacttttag aacaatttaa tataaaggag tgtgacttag
     4141 taatactaga tataatgatg cctggctcaa gtggttttga agtatgtaca aaacttcgtg
     4201 agaaaagtac agtcccaata atcatgctta ctgcaagaga tacagatata gattatatta
     4261 cagggataac cttaggtagt gatgattatt ttacaaaacc atttagccca atgtctttgg
     4321 ttatgagagt aaaatctata tttagaagga tagagtttga aaagaaacaa aattatgata
     4381 aatatagtaa ctcgatagat atggaattaa aatttggtga tgtaataata aataaaaaga
     4441 ataaaatcgt tacaagtaaa aatgtaaata tagaccttac accaaatgaa tataacctat
     4501 tgacttattt gtttgaaaat atagatagag cagtttccag agatgaatta ttaaacaaaa
     4561 tatggggata cgatatagaa gttgagacaa gggctgctga tgatacagtg aagagattac
     4621 gtaaaaaaat tttaaataca aatattttaa tagaaactgt atgggggttt ggatttaggt
     4681 tgaaggagaa aagttaagat gaagaatgga aatgacaaga atagaattgg aaagattaaa
     4741 tccaaattta aattaaagtt ctcaaagaaa aatataagca taaaaactag gattttgttg
     4801 agtatacttt gtttgataat tatagtattt atgataatat tagtatcttt taatgtattt
     4861 cttgatacat atataaaaac aactgcgaat gaagaattga caaagtctac agaaatagta
     4921 gaacatatgg atagtaattt tagacctatt aagccaccac aagatgatgg aaagcttcct
     4981 cccaaagtat tatctaattt tatgagaaat gtacaagata aagttaggat ggcagaaaca
     5041 caatcagatg ctgatgctat ggtagttgat tctaagtata acctgatatt tccaacaaga
     5101 gaggatgatt tcttaaaaaa tgttgatgag atggagctga ttagggagtg tattcaaaat
     5161 gaaaaattag atttaaattc agatgaaaat acacggatat ctaccagaaa taaggattat
     5221 tatatttcaa ctgtaaaaat tactagtata tatggtgaac aagacaagta tcttatactt
     5281 tttatagaca tatcaaaaac tctaaatcta gcacaaaaga taaatattgt attaatatct
     5341 gtaatgtgtt ttgctggaat attagcaata tttacagctg tgattttatc tgagaaaatt
     5401 gctaaaccga taaaagagtt gtgtgagttt gcaaaaaaaa tgggtcaagg agatttcaaa
     5461 agaacctact ttgacttttc agataaagaa ttagtagagc tctcaactgt tatgaataag
     5521 agtgctgagt atctggataa gtatgataat gaacaaaaaa tattttttca aaatgcatct
     5581 catgaattaa ggacaccact tatgtctatt aaaggttatg cagaagctat aaaatataat
     5641 gttatagata gtaaacatgc aagtgatata attcttgaag aaagtgatag gttaagtgat
     5701 atggttgaag atttattata catttctaaa atagataata taactaaaga ttttgaactt
     5761 gtagaatgtg atttaagaga agtactttca aattgtggag ctaggcaaaa tgtaagagct
     5821 ataaataagg ggataaaatt tatttatgaa tttgatgaag aaatggtttt atttgaatgt
     5881 gatgaaaaaa acatttccaa agcatttatg aatcttatag aaaatgcttt gagatacgct
     5941 aagagtgaaa taaaaatagt gtgtaagtat aatcaaaaaa atatagttgt gataatagaa
     6001 gatgatggaa ttggaatcaa gaaagaagat ttacctcatg tttttgaaag attctataag
     6061 ggagtaggag gaaatcatgg aattggatta tcaatagtta aatctattgt aaataagcat
     6121 ggtgggcgta tatacgtaga aaatggcaaa aaaggagcta aatttactat attatttaaa
     6181 ttgtaaaaaa attagagaaa atatagagat gtttgaagtt gttttttata tatatctgtt
     6241 gtatactgtt gaaaaaagaa caatattaag ctgacatata ttacgatagt tgacatttaa
     6301 agtaaatgct taaatataga tacgaaagga aaagtaaaat ctgaagtgta tttaggttta
     6361 agtaagcttc ttattttata aaaaatatgt atgaaagaac agctgatttt taatattaca
     6421 aatttagtaa cggttgtatt tgaagctttt gtcatccata tgtttttatc agacttttta
     6481 ggtcagaaag aagggtatat aaaagttgta agatatgcta aactgtgttt tataatatgt
     6541 ttaggattct gtaatttaat aactttaaac cctaaaatca caatgccttt aattttcatc
     6601 cttatttttt caacatcatt tttatataaa ggaaatttaa agacacgatt gtttactact
     6661 gttttattaa gtatattctt tatactgtca gaaattgtag ttacaagtat ttttgtttta
     6721 tttgtaaaag aaggctttga aataatgctt gaaaataata gtataagagt attggcgaca
     6781 attttatcta aaattgtctt tttgttgact tgtaaaatta tttgtctatt taaaaaggat
     6841 gtacatttag atatgcccat aaagtattgg ctccctttat ttttaatacc tatatttagt
     6901 ttatttctgt ctgtttctat ctttgatgta agtaaatttt ttagtcttga aagtttaaaa
     6961 tttctaagct taatatctag tgttggtatt ttatatatta attttattgt cttttattta
     7021 tttaaattta ttatagataa aactaaacta tctatgaaat atgaactact tgaaaaagaa
     7081 ataattcata aggaagaatt aagacttagt aatgagtgtt ataaaattat tgttgaacag
     7141 actgattctg tcgttttcga atggaatatc aaagaaaata aatcatttgt atcgcaggct
     7201 tggactgaaa aatttggata taacaatgca tgtaagaata tttttaaaga aattaaagat
     7261 aaagacttag ttcattctga agataaagct atatttgaag gttttcttga aagtattaaa
     7321 aaaaagaata tgcacaatca agcagtatat cgtttaaaaa aatctaatgg tgaatatatt
     7381 tggtgtagaa catcaattac aagcatttat aatgatgaaa atgaactttt aagagttgtc
     7441 ggagtaattg tagatgtaga tagtgatata aaaaaatatg aagagttaag aactagagca
     7501 gaaagtgact cattgaccaa tatatacaat aaaggtacat ttgaaaaatt agtggaagaa
     7561 actatagtta tgaatacagg tgataaaaaa gatgcattat tcattattga tttggatgat
     7621 tttaaagaaa taaatgataa ttttggtcat ccatttggtg attttgtact aaaaacattt
     7681 gctgataaga tacaaactag ttttggtagt aaagacttag tcggaagaat tggtggagat
     7741 gaatttgttg tatatatgca agattatgtt acagaagtaa atttgcataa aaaagctaaa
     7801 gaattaaata gagtactttc ggataattac actgatttga gttttagttt tgatgcttct
     7861 gtcagtatag gtattgcaag gtatcctcaa gatggaactt cattttttga attgtttaaa
     7921 aatgcagata gagcacttta tagcataaaa gcatcaggta aaaattcata ttgtttattt
     7981 gaagaagaac tatatgtaca gtgaatttga atttagattt tatgtgagag taagatttat
     8041 atatacttgt ggctgtctga tttataaaat atgttacttt gtatattttt atatgtttca
     8101 gtaaggttaa ttaacatgag caaaggtgta agtaaattta tattagttta agtttactta
     8161 cacctttata aaaatacaaa taatagattc atttacattc ttaaatgatt aaagctcttt
     8221 accttctaaa catgcataac atatcgttat aattccagta aataagacta aaatgcccat
     8281 tactaagatt acaatttcat catctattac tccaaatgtt tttaaagtaa cttatttcgc
     8341 tttgtatcat aataatttgt ctctttttac attttaactt ctgctgagat ttgagttaga
     8401 tattcattag aatctcgcac acttatatca tatagaagat attcttcata aacaaaatcc
     8461 ccaatacgta aattattctg attaaaaaaa tttaataact tttcataagc cttgtaaata
     8521 gtttcataac tccctttatg ataagcagtt acataaagtc cctttggttt gattgataca
     8581 ggatatttct tgtactcttt attaactttt gtaaataaat atgaatatga ttcaaagtct
     8641 ttattaatta tatcttcacc tttaatgatt gagccaatcg taccataatc actcaattca
     8701 tagttgtcca taaagttaat acattcatat aaaaaatttt cttcctcatc tcctaaaaac
     8761 tgttctttat atgtaacagg tgttttgacc aagtactctt cttcatgttc ttttaaagta
     8821 atagtattcg catctatatt acatgcactt ttagtaaatg aaatagtaga ttccattaga
     8881 gcttgaatat tatttagttt ttcaatttca tttttaatat caatggtttt cttttctaaa
     8941 agctcaatta gtaaattagg tgttcttttg tctatataag ctttaatttc ttttaaaggc
     9001 atttttactt ccctaagtga agaaattata ccaaaaatgc taagttgatg gtatgagtag
     9061 tatctatatc cattttcttt ctttaattct ggtgagaaaa gacctatatc atcataatgg
     9121 aaaagtgttt ttttattgat tccacaaatc tttgcaaatt ctccagttgt aaaataattt
     9181 tttgaaactt gactcataat tataattcct ccttgactat cctgttacag tatagtttat
     9241 catagtattt tgtaaaggag tgtgattata tgaaaaaatt aattcatttt ttaaaaccat
     9301 atagagtttt aattgttgta gttttgatat ttacgttttt acaaacactt ggaactttgt
     9361 atataccaac acttacagca aatatagtaa ataatggtgt agtaaaggga gatatagatt
     9421 atattgtaaa aacaggttta atgatgatga tagtagctgg tataacagct ttatcagctg
     9481 tgttagtttg taaagtatca gcaaatcttt catctggatt ttgtagagat attagagaag
     9541 cagtatttat aaaatcacaa gatttatcaa taaatgattt taataatata ggtacagcat
     9601 caatgattac taggagtact agtgatatta ctttaatagg tcagtctgtg tttatgttta
     9661 ttcaattagt attaccagca ccaattataa ctgtttctgg tctattctta gcatattcta
     9721 tagataaagc aatgacaatt attattgttg tagtaatgtt tttattcatg ctttcagcat
     9781 ttttagttgg taaaaagctt ataaagttgt ttaaaatgat gcaaataaag atggataata
     9841 tgaatagggt gttacgtgag gtagtaactg gagtaagagt tataagagct tttaatagga
     9901 gtcgttttga aaaaaagaga tttgatagaa ctgcaataga ttattctgaa acagcaattt
     9961 caataaataa aatatttgca gtacttatgc ctattgtaat gttaataatg aatttaggta
    10021 tagtaagtat tatttggttt ggtgggatga gagtcagtaa tggaaatatg gaaataggtc
    10081 atataatggc acttgttgaa tattgtatct taattttatt ctatttaatc atgggagtaa
    10141 tggtatttat gtacatacct agagcaggtg catgtgcaga tagagtaaat cagatattag
    10201 atatagaacc tgaaattgta gatggaaatg gtcataaaga tactgtctca gagagaggac
    10261 atttagtatt taaaaatgta accttttcat atgcacaatc agaagaacct gtgttaaata
    10321 atataacatt tgaagcaaaa tctggagaag taactgcaat tataggaagc actggctcag
    10381 gcaaatctac tattgcaaat ataataccac gtttttttga aattcaatct ggagaaatat
    10441 ctataaatgg tcaggatatt aagaagattc ctcaaaaaga acttagagat aagattggat
    10501 ttgtaccaca aaaagctttt ttatttagtg gaacaataga agaaaatata aggtatggaa
    10561 aagaagatgc aagtattgaa gaagtaaagc atgctgcaag tatagctcaa gctgatgaat
    10621 ttatttatga tatggaagac aagtatgact catttgtagc acaaggtgga aataatttgt
    10681 caggtggtca aaaacagaga atatccattg caagagcgtt agttagaaag cctgaagtat
    10741 atgtctttga tgatagtttt tctgcattag attttaaaac agataaaaga cttagaaaag
    10801 cgttgaaaaa tgaaatcaaa gattcatcag ctataattat agcacaacgt ataagtacaa
    10861 tcatggatgc taatcagatt atagttttaa atgatggaaa aattgtaggt attggaaaac
    10921 ataaagattt acttgaaaat tgtgaagttt ataagcaaat tgcggattca caacttagta
    10981 aagaagaact tgcataaaat gaagaattaa tctgatattt ggaggtgtat gatatgagtg
    11041 aaaaaagaat agatgaacaa gatatagaag tacttagttt tgaagatggc atgtcagcag
    11101 aaaaagctaa ggatgcaaaa aaaactacta agagattatt aaaatatatg gcaaaacaaa
    11161 agtttaaaat attaattatt tttatttcag tattgataag ttcagcgctt acagttttag
    11221 cacctatggt tatgggaaaa gctattgacc aactatttaa tggaataaaa actgcagtcc
    11281 aaacaggtac aaaattcagt gtgaatttta gtactatggg agggatagta tcaatacttc
    11341 tagggttgta cttaattagt gcagtattta tatatattca acagtatatt atgtcaggag
    11401 ttgcacaaaa tctagttctt agcatgagaa aagatttaag tgataaactc aataaacttc
    11461 ctcttaagta ctatgattct cataaaaagg gggaaacact tagtatagta acaaatgatt
    11521 tagaaaaagt agcagatagt ttacaagaag gattaatgca acttattaca gcagtagtta
    11581 cagtagttgg ttctattgta atgatgataa gtataagtgt accacttaca atagtatcag
    11641 ctataacatt acttgtttca ttgggtatta ctgttgtaat tgcaagaaaa tcacaagaaa
    11701 gattctctga aaatcaaaag gcactaggag agttaaatag caatatagaa gagatattta
    11761 ctggtcaaat agtggttaaa gcatttagta aagaaaagga tactataaga aactttaaaa
    11821 tattaaatca aaatctttat aatgcaagta gaaaagcaca gttttcgtca tatgcaatat
    11881 ctccaattat tagatttata aacctaattg gctatgttat tatagcagtt gtaggtggga
    11941 tatttgcatc aactggagca atgactcttg gtagtataca agcgtttatt caatatgtaa
    12001 atcaagcatc agagcctact acagaaatat cttatattgt aaatatgctt caagcagcaa
    12061 tagcatctgc agagagagtt tttacagtta tggacgaggt ggaagagata aaggatgaag
    12121 aatcttcaaa ggttattagc atgcctaaag gtaaagtaca atttgagcat gttaaatttg
    12181 gatatagtga tgattttatt cttatgaaag atattaatat caatctaaat gcaggagata
    12241 agattgcaat tgttggacct acaggagctg gaaagacaac tcttgtaaat ttacttatga
    12301 gattttatga gatacaaggt ggaagaatta ctattgatgg tgttaatata aaagatttaa
    12361 agcgtggaga acttagaact atgtttggta tggttcttca agatacatgg ctatttaatg
    12421 gaagtataaa agaaaatatt gcatatagta agtcagatgc cacaatggat gaaattgtaa
    12481 gagcagcaaa atcagcaaga gtagaccatt ttataaggac tttaccacaa gggtatgaca
    12541 ctatattgac tgaggatgct tctaatctat ctcaaggtca aaagcaactt ctaacaattg
    12601 ccagggcaat actttcagac ccatcagtat tgattcttga tgaagcaaca tctagtgtag
    12661 atactagaac agaggtagaa attcaaaagg caatgaataa cttgatgaag ggaagaacta
    12721 gttttgttat tgcacataga ttatcaacta ttcgagatgc tgatttaata ttagtaatga
    12781 aagaaggaac tattattgaa caaggaagcc ataaggaact tattgaaaag aaaggattct
    12841 atgaagaact ttataacagt caatttacaa gtgaatatga tgaagatgtt gtatgattat
    12901 caagttttag gcaactaaat gattgataaa tattaagtta aatatagtaa catgtaaggt
    12961 tatatggaat aagagtattt ctctatttca tataaccttt tttatttatc aaaataagat
    13021 aaaatttcct ttctaaatta gattgaagaa tgtatttatc aatataaaag taaaataatc
    13081 attattatta gtgaagtaaa tattgtttgc cacatatatg ccataaagat gcacttattt
    13141 tacggagatt taacaaagta gtgtaaagta tactctaaat ataaaataaa tcaaatagag
    13201 ttaaataaat tgagaggtga tataaatagt gaataaaaaa ttgaactata gatttgaaat
    13261 gaagcacaaa ataactgaag cagatgtgtt agctttaaaa tcaagactct atccaataat
    13321 gaagaaagat gaaaatgcat ctaaagatgg aaaatatctt attaggagtt tatattttga
    13381 tactcctgaa gataaagcac ttttggataa attaaatggc gttgcaataa gagaaaagtt
    13441 taggataaga ttttataata atgactgctc ttatataagg ttagagaaaa aaatcaaaca
    13501 ttacaacatg acatcaaagc tatcaactaa tttaaccaaa aaagaagtaa taaatatact
    13561 aaataatgat attgaatttc taaaagagtc gacaaattgt ttattaagag aattttattt
    13621 gaaattaaaa tgtgaaagat tagaggcaaa aagcatagtt gattacaata gagaagcttt
    13681 tatttatcca gttggaaatg taagagttac aatcgatagt gatattaaaa cttctgtaaa
    13741 ttcggtagat ttatttaata aagatttgcc aactgtttcg gtaattgatg agaatatgac
    13801 tgtattggag gttaaatatg atgaatttat accagatttt ataaaggatt taattcaaat
    13861 taataagact acctctacag cagtttcaaa gtatgcttca agtagattat atatttaaga
    13921 ataaaggcta tgatttatat aaagatatac ataaataaga ttaaaaaaca aataagttaa
    13981 aaataaaaaa taaataagtt aaaaatagga aggatgattt taattatgac atttaatgat
    14041 atttttaaat caagttttat agaaaacgta tcaggttttt catttgtgga cagtgcttta
    14101 gcactaggtt cagctttttt agtaggtttg tttatatata tggtctataa aaagacttat
    14161 atgggaatta tgtattctcg accattcaat gtgtcgcttg tagcacttac aatgcttaca
    14221 acttttgtaa tattggcagt tacttcaaat gtagttttat ccttaggtat ggttggtgct
    14281 ttatctatag taagatttag aacagcaatt aaagacccaa tggatttagt atttctattt
    14341 tggtcattgg gttcaggtat agttttagga gcaggtttga ttcccttagc tgtcatgggt
    14401 tcaataatta tgggattgat tctaatattc ttttcaaata aaacaatatc tgaaactcca
    14461 tatatattga tggtaaattg taataatgaa gattcagaag atatagcaac tgataaaatt
    14521 aaaaaagtat ttactaaata tcaaataaaa tcaaaaagtg ttacaccaga aaaaggaata
    14581 gaattagtgt ttgaagtaag aatgaaagat ggagaaacaa gtcttataaa tgacttgagt
    14641 caagtagatg gagttactaa tgcagttctt gtaagttata atggtgacta tgtagcatag
    14701 agaggagtaa tcttttatga aagataaaaa atttaccctt cttatctcga ttatgattat
    14761 atttttatgt gctgtagttg gagtttatag tacatctagc aacaaaagtg ttgatttata
    14821 tagtgatgta tatattgaaa aatattttaa cagagacaag gttatggaag ttaatataga
    14881 gatagatgaa agtgacttga aggatatgaa tgaaaatgct ataaaagaag aatttaaggt
    14941 tgcaaaagta actgtagatg gagatacata tggaaacgta ggtataagaa ctaaaggaaa
    15001 ttcaagcctt atatctgtag caaatagtga tagtgataga tacagctata agattaattt
    15061 tgataagtat aatactagtc aaagtatgga agggcttact caattaaatc ttaataactg
    15121 ttactctgac ccatcttata tgagagagtt tttaacatat agtatttgcg aggaaatggg
    15181 attagcgact ccagaatttg catatgctaa agtctctata aatggcgaat atcatggttt
    15241 gtatttggca gtagaaggat taaaagagtc ttatcttgaa aataattttg gtaatgtaac
    15301 tggagactta tataagtcag atgaaggaag ctcgttgcaa tataaaggag atgacccaga
    15361 aagttactca aacttaatcg ttgaaagtga taaaaagaca gctgattggt ctaaaattac
    15421 aaaactatta aaatctttgg atacaggtga agatattgaa aaatatcttg atgtagattc
    15481 tgtccttaaa aatatagcaa taaatacagc tttattaaac cttgatagct atcaagggag
    15541 ttttgcccat aactattatt tatatgagca agatggagta ttttctatgt taccatggga
    15601 ttttaatatg tcatttggtg gatttagtgg ttttggtgga ggtagtcaat ctatagcaat
    15661 tgatgaacct acgacaggta atttagaaga cagacctctc atatcctcgt tattaaaaaa
    15721 tgagacatac aaaacaaaat accataaata tctggaagag atagtaacaa aatacctaga
    15781 ttcagactat ttagagaata tgacaacaaa attgcatgac atgatagcat catatgtaaa
    15841 agaagaccca acagcatttt atacttatga agaatttgaa aaaaatataa catcttcaat
    15901 tgaagattct agtgataata agggatttgg taataaaggg tttgacaaca ataactctaa
    15961 taacagtgat tctaataata attctaatag tgaaaataag cactctggaa atcaaagtga
    16021 tgaaaaagaa gttaatgctg aattaacatc aagcgtagtc aaagctaata cagataatga
    16081 aaataaaaat aaaactacaa atgatagtga aagtaagaat aatacagata aagataaaag
    16141 tggaaatgat aataatcaaa agctagaagg tcctatgggt aaaggaggta agtcaatacc
    16201 aggggttttg gaagttgcag aagatatgag taaaactata aaatctcaat taagtggaga
    16261 aacttcttcg acaaagcaaa actctggtga tgaaagttca agtggaatta aaggtagtga
    16321 aaagtttgat gaggatatga gtggtatgcc agaaccaccg gagggaatgg atggtaaaat
    16381 gccaccagga atgggtaata tggataaggg agatatgaat ggtaaaaatg gcaatatgaa
    16441 tatggataga aatcaagata atccaagaga agctggaggt tttggcaata gaggaggagg
    16501 ctctgtgagt aaaacaacaa catacttcaa attaatttta ggtggagctt caatgataat
    16561 aatgtcgatt atgttagttg gtgtatcaag ggtaaagaga agaagattta taaagtcaaa
    16621 ataagattaa aattgaatat aaataatttt aatactgtta agtctaaata tttacttagc
    16681 agtatttttt agaaaataat gtatttggtt actaatataa ggtaataaat tgataaaaat
    16741 t
//
LOCUS       DAMKMI010000090        16132 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMKMI010000090 DAMKMI010000000
VERSION     DAMKMI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.093"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            77..2188
                     /locus_tag="PC429_002546"
     CDS             77..2188
                     /locus_tag="PC429_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF3848045.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(2386..3351)
                     /locus_tag="PC429_002547"
     CDS             complement(2386..3351)
                     /locus_tag="PC429_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF3848046.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(3636..4592)
                     /locus_tag="PC429_002548"
     CDS             complement(3636..4592)
                     /locus_tag="PC429_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF3848047.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            4960..5748
                     /locus_tag="PC429_002549"
     CDS             4960..5748
                     /locus_tag="PC429_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3848048.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(5871..6341)
                     /locus_tag="PC429_002550"
     CDS             complement(5871..6341)
                     /locus_tag="PC429_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF3848049.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            6480..7124
                     /gene="uppS"
                     /locus_tag="PC429_002551"
     CDS             6480..7124
                     /gene="uppS"
                     /locus_tag="PC429_002551"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF3848050.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            7167..8072
                     /locus_tag="PC429_002552"
     CDS             7167..8072
                     /locus_tag="PC429_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3848051.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            8387..9934
                     /locus_tag="PC429_002553"
     CDS             8387..9934
                     /locus_tag="PC429_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3848052.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            9937..10335
                     /locus_tag="PC429_002554"
     CDS             9937..10335
                     /locus_tag="PC429_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF3848053.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            10375..12108
                     /locus_tag="PC429_002555"
     CDS             10375..12108
                     /locus_tag="PC429_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF3848054.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            12340..13551
                     /locus_tag="PC429_002556"
     CDS             12340..13551
                     /locus_tag="PC429_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF3848055.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            13744..14004
                     /locus_tag="PC429_002557"
     CDS             13744..14004
                     /locus_tag="PC429_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF3848056.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            14358..16070
                     /gene="ptsP"
                     /locus_tag="PC429_002558"
     CDS             14358..16070
                     /gene="ptsP"
                     /locus_tag="PC429_002558"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF3848057.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT         6482 a         1692 c         2687 g         5271 t
ORIGIN      
        1 ataatatgaa ttttagatat acttaataag aagtatattg tagaaaaaat aaatttaaaa
       61 aattaagggg gatgaaatga aaaaaataag tatattagta ttatcattga taatgacact
      121 tactatgtgt agtgtaagtt catttgctga ttcatcaaat gataaggaaa tgagagctgc
      181 ttggatatct acagtttata atttagactg gcctaagact aagaataatg aagccaagca
      241 aaaaaaggaa tacacagatt tattggacaa attaaaaagt gtaggaataa atacagcagt
      301 ggttcaagta agaccaaaat cagatgcttt atataaatca aatataaacc catggtctga
      361 atatcttacc ggtacacaag gtaaagaccc tggatatgac ccattaccat ttttaataga
      421 agaagcacat aaaagaggaa tggaatttca tgcatggttt aatccatata gaataactat
      481 ggcagatgag agcatagata aattgccagc aaaccatcca gcaaagaaaa atccaagctg
      541 ggtagttaaa catggaaata agtattacta cgacccaggt ttaccagaag ttagaaagta
      601 catagtggat agtatagcag aagtagttca aaattacgat atagatggag ttcattttga
      661 tgattacttc tatccaggag taagtttcaa tgatactgca acataccaaa aatatggaaa
      721 aggacaaaat aaggatgatt ggagaagaga aaatgtaaat actctactta gagatgtaaa
      781 agcgtctata aagagtataa agcctaatgt tgtatttggt gtaagcccag caggtatatg
      841 gagaaataag agtagtgacc caacaggttc agatacatca ggtaatgaaa gttatgtagg
      901 tacatatgca gatacaagag catggataaa acaaggattg attgactatg tagttccaca
      961 actttactgg ccaattggtc ttaaagcagc agattattct aaattagtgg catggtgggc
     1021 taatgaagta aaaggaacaa atgtagactt atacatagga caaggtatat ataagcaagg
     1081 tcaatcgagt tatggtggac aaaatatagc aaaagaaata gtacaacaag taacattaaa
     1141 tagaaagtat agtgaaataa aaggtagtat gtatttctca gctaaagata tagcaaatag
     1201 tacttctata caaaaggatt taaagagttt atattctagt agtgaagaac cagttactcc
     1261 accatctaat gttaaagtgg agaaattaag aggcgacgaa agatatgaca cagcagtagc
     1321 aataagtaaa aaaggttggg ctacaaattc agatacagta gtattagtaa atgggtactc
     1381 tatagttgat ggtataacat caacaccact tgctacatca aatgatgctc ctatactatt
     1441 ggtaaataag gataatatac ctacatccac taagaatgag ttaaaaagac ttaatccaag
     1501 taaagttatt ttaattggag gaaataactc tattggagat aaagttgaga gtgagataaa
     1561 agatacattg tcaaatgtat caataaatag agttggtggt tcagatagat atagtacatc
     1621 tttaatgata gcaaaagaat tagtaaaaac taatccagta gaaaaactat atataacaag
     1681 tggtacaggt gaagcagatt ctctttctat agcttcaaaa gcaggagaag aaaagcaacc
     1741 aatagtatta gtatcaaaag ataatgtaag tgatgaagtt tacaattgga tatcagattt
     1801 aaaagtaaaa gatgcttatt ttataggtgg taatctaagt atatctgatt cagtaataaa
     1861 taagctggat aaggtcatta caaatgatgt atctaaaaat agaatagctg gagaaaatag
     1921 agaagaaaca aatggaaaag taatacaaaa attctatcca aatgctgaat attcatcaat
     1981 gtttgtaagt aaaagtaatc aattggtaga tgcattaaca tcaggtcctt tagcagctaa
     2041 attaaaatca ccagtagtta tgcttggaaa tagtgtaaca tctgctcaga aaactgcttt
     2101 agagcctaag aaaactactc tagtatacga agctggagat ggtataaatc aaaacacatt
     2161 aaacactttc ttaaacttag ttaagtaata aaaaagttaa tttaaaatac agagtatttt
     2221 ataaatgtag attaaaatga aaattatcat gataaattag aaaataaaaa agaactatat
     2281 gcattttgta atcaagtgta cacagcaact tgaaaataat tatgcgatat agttcttttt
     2341 tagtaattaa tcttatactt tttaataaac tttatgttga attttttatt gtttaaactc
     2401 tatgtcatta aatataaaat cgtgtaatat gtctaaagta tctgggtcaa atctaagaac
     2461 ccatccagct tttccatata taccacctgt actatgaatt tcatcatcaa taggaaattc
     2521 aaattgctta atatttaagt cgcccatttt taaaacttga gctcctaaag aaagtattgc
     2581 attaggcttc atgttagttt taacatatgg aagaactgca tctaataaac ctggatattt
     2641 agtaacaggt aaagtttccg ttttcttgat tatagcttgt ataatctgtc tctgtctgcc
     2701 atctctagca aaggctgtat cgttgtgtct gatacgtgca aaagatagtg cctgatatcc
     2761 atttagagtc tgctttccag agtttctaat gtattgtatg ctacctttat catcactttt
     2821 attccattta taagtttcag gaataaattt atttagttct cgtatttctc ctttttcaac
     2881 agtaacctca acgccaccaa tagcatcaat tatatacata aatgattcga aattaacaca
     2941 agcataattt tgaatatcaa tattaaaatt ctcttcaatt gtttgtatta ataaatcagc
     3001 ttgtccataa gcataagcat gagtaagttt ttgtttacca tgaccaggga tgtctacata
     3061 gctatcacga gctaatgatg taagtttgat atcattgtgt ttgttatcaa tagtaagtat
     3121 catcatagca tctgaccttg aagaatcttc atttggtcta gcatctgttc ccataagtag
     3181 tatgttaatt atcccatctt catttttatg gtcattgtta tccaataaat cactacttat
     3241 gctcgagtca tgaatagcag ataatttata ataaaaatat ccatagacac ttatagggaa
     3301 tattactacc aagaaagcca gtagtataac aaatttcttt aattttgaca aaagtacccc
     3361 ttctttcttc tttttcctgt atatttttta atagcataca cttaattata ttaaagtatt
     3421 gagatatatt caatacttgt ttcagttaca aaaaaaagac aatgatataa tttataagaa
     3481 aatttatcga cctatatata tagttaaaag tatatataat atatttttta aaagtaattt
     3541 atattttaaa ataaaaacac accccataaa tacaaagtaa tttgcattta agaggtatgt
     3601 atttatcatc aaattattgg tttgttttgg aatttctaat cttcaaccat aatatcttta
     3661 aatatgaaat catgcattgc atctagttca tattcctcaa aaggtattac atagcctgca
     3721 ttacctattt ttctaccatt ttcagtagaa agaggaaact ccatagattt caaatttaaa
     3781 tttcctatac ttaaaagttc ttttcctaaa gatattattt cattaggatt catattagtt
     3841 tttacatacg gaagtattgt gttaactaga ttaggatatt tagtgactgg taaatcctta
     3901 acaccattta ttaaactctg tataacactt ctttgtcttc tatctctttc atctgtacta
     3961 tcattttttc ttatacgaga aaatgcaagt gtctgatatc catttaattt ttgctcacca
     4021 gaatttctta tatatttaag agaaccttta ttgtcatttt tattccattt ataagtttca
     4081 ggaataaatt tatttaattc tcttatttca ctcttatcaa cagtaacagt aactccacct
     4141 aaagcatcaa ctatatccat aaaagagaaa aagtcaacta cggcgtagtt ttgtatatca
     4201 agttggaagt tttcttctat agtttctatt aataagttag cttgtccata tacgtaggca
     4261 tgtgttaatt tttgttctcc atgtcctggt atattaacat aagtatctct atttaatgaa
     4321 gttagcttaa gacttttatt tttattatca atagtaagta tcatcatggc atcagaacga
     4381 gaaacttctt ctcctggtct tgcatctgta cctaacaata atatgtttgt gatattttct
     4441 tcgtttttat agtcattact actaagcgcc tctgtattta tactatcatc ttgtatagtt
     4501 tttaatttag taaaaacata tccatatata ctaacaggaa atgctataac taaaagggct
     4561 agaaatataa cgaacttttt gagtcctgac aataatatcc cctactttct aaatttttta
     4621 attacacata gttaattata ttaaaaattt gttacataag caataaaaaa cacaataaat
     4681 tagcataagt ttgtcataat ataattattt ttatagcatt ttatagaaga aagtgtgtag
     4741 ttattattga ttttattgga atataattgt atgaagactt aaatttttat aaactaatgt
     4801 gtatattgaa aaaaatattt tcctgaatgt agttatagca tcttatgaaa ggtgcaaatt
     4861 gtaatttaaa atttttagtg tataataaga attgaaaatt caaaccgata aaaattaata
     4921 aaaaataatt ataaattaat aatttggggg aggaaatgta tgataaaatt aatagcaaca
     4981 gatttagatg gaactttgtt agatgaaaag agcgaaataa atccagagtt ttataaagta
     5041 tttaaaaaat taagagagag agggataatg ttttctgctg ctagtggcag gcagtatcaa
     5101 aatctgataa aaaaatttga agatataaaa gatgatatga tgtttatatc agaaaatgga
     5161 acactagtag tatataaagg aaaagaaata ttatcaaatc ctttaaataa agaattagta
     5221 aatgaaatca tagagacaac aagaagtata aaagggaaaa aaattgtaat gagtgggaaa
     5281 aaatacgctt atatagaaag caaagatgaa gcgtttatac aagaggttag tacttattat
     5341 gcaaagttta aaattgtaga agatttgacg aaagtagagg gagacatatt gaagatagct
     5401 gtgtttgact tcaaaggtgc agaacataat aataatatct actttgaaaa gttttctgat
     5461 agagctcaag tatgtatttc tggagtggag tggcttgatt taactgcaaa aggtgcaaat
     5521 aaagggagtg ccataaaaaa agtacaaaaa atgttagata taaaatatga agaaactatg
     5581 gtatttggtg accagttaaa tgatgtggaa atgatgaaaa gtgcctatca tagttatgcc
     5641 atggaaaatg caaatgaaca tttgaaacaa attgctagat ttagagcaaa aagaaatact
     5701 gaaaatggcg ttgtagataa gataaaagag gttataaaaa ttggatagtc atatgtatag
     5761 atgaaaaaga ttattttgaa gaaatcaaaa aaatatcaat gtaattttga taaataagtt
     5821 tatttatgcg aaaattacat tgatatttaa taaacatcaa taatagatat ttattttttt
     5881 atgatattca gtattttgtt ttctatttga gttgcatttt cagcagaaat taaataaaca
     5941 tacctacctg ccttctcaat tatagtatta tttgctattt cagaattgat tttttctcca
     6001 tgtccttctc caaaaggtgc ttgtatcata tcttttttat aattttctag agttttatag
     6061 attttatcta cctcgtcagt ttttataacg ataatatctt ctagagataa tttttcagaa
     6121 gaattaatta caaaaccttg aattatgtct tcctctacat cattaaaata gttaaaatcc
     6181 tttgcatcaa agatttttga ttcctttata agtaaatcag aagattttaa tgcttttttt
     6241 atttttgatg tagaaacgtt tttgtaatca ccacttgaag aacaactaac cattcctaat
     6301 gataagatta tcgaaaaaaa caatattaaa atttttttca tgataaacct ccaagttttt
     6361 taaactctgt cttaaattag tttactacat aattatataa aaataaatgt atttaatgtt
     6421 acagttttga ttaataatgt aatattttga taataatacc tattgagagg tgattagaaa
     6481 tgagaatacc aaaacatgta ggaattatac cggatggaaa tagaaggtgg gcaagtcaaa
     6541 atggtatgac aaaagataaa ggatatgaaa gtggaataga ccctggacta gagtcttata
     6601 gattatgtag agatttaggc tgtgaagagg tgacttttta tggatttaca actgataata
     6661 caagacgtcc atcagtgcag acaaaagctt ttacaaaagc atgtattgat gctgcaaata
     6721 tattaataga tgaaggtgct gctttattag ttgtaggaaa tacaaaatcg ccaatgtttc
     6781 cagaagaatt attaccatat actaatagaa ttaaagccaa agaaggtcaa ataaaagcaa
     6841 atcttctggt aaattatggt tggtattggg acttaaataa tatatcagga gtagaaaatg
     6901 taaataaaaa taatatacag gattacatac agtcatttga tgtatccaga ctagacttga
     6961 taatcagatg gggtggaaga aggagattaa gtggtttcct tcctgtacaa tcaatttatg
     7021 cagattttta tgttatagat aattattggc cagattttaa gagtgacgat ataaaggacg
     7081 catttagttg gtatgataca caagatgtca ctttaggtgg ataattaaaa atattctgat
     7141 ttaaggttgt agagtgtttt aaattattga aaaaaaaact tcttgatggt aaaataacac
     7201 taataatatg taaaagtgta aaaatttata ccaagaaggg tgaagaaatg tcaaaaggca
     7261 ataataacaa taattcaaga aacaaatcta aaaagaccag tcatctgaat agaaaaaagc
     7321 gaaaattaaa taagaaaaag ttagcagtgt tgatttgctt tacagtttta tttttattta
     7381 ttgcatttaa ggctactcaa ggtgtagtag ctttggtaaa aagtatggat aaaagtaata
     7441 aaactagtca acaacaaaat gttaactcag aacaatttga ccttggtaat gaagaggaaa
     7501 ataaaaagaa aaaatatact gtatttatag acccaggtca tggaggcaat gataaaggaa
     7561 ctgaaagcaa gaccagtaat agatatgaaa aagacctaaa tctacaaatt gcaaaaaaat
     7621 tagcaaataa attatctaaa caaaaggata ttcaagtagt agtatccaga actgatgata
     7681 catatatatc attaaaggat agagctatct tagcaaataa tagttctgct gatgtgcttg
     7741 tttctattca tttaaatgca gagaaaaatg gtaatacagc aacaggtata gaaacttggt
     7801 ataggaataa agctacagat ggttcaaaag aacttgcaca gacagtacaa agtactatag
     7861 tatcctatgt taaagttaga gataggggaa tagttgaaaa taactttgaa gtacttagag
     7921 aatcaaatat gcctgctata ttaatagagt gtggtttttt aacaacacct tcagaggaac
     7981 aaaaaataat aaatgaaaaa tatcaagacc aacttgcgga aggaattgtt caaggtgtac
     8041 tttcatacct agattctaaa gggaataagt aaattaactt tactttaatg tgaggataaa
     8101 tttaaaagtt taactgtgta aattgtatta actttatttc gtaatgttgg aaatattgtt
     8161 tgaatttaga aactatgaaa ttagaaatta ttttatctat atctaatttc atagtttttt
     8221 ttgcaaaaaa tttatagtta taaaatttta aaaaataaat ttagtataat gtaatgtagt
     8281 tatttatatg tgattttaac ttgcattgat ttgctatcaa ttgtgcttta taattagtat
     8341 atataaagtt taactgtaag ataatttaaa aaatagggga atttatatga ataaaaatag
     8401 catgacaaga atttacagtt taaataaaat aatttttatt ataattggaa ttttaggaat
     8461 agttttactc acaagacata ttgaggtaga tattaaagtt ttaattttaa ttttattaac
     8521 cttgtttagt ataaatgtat ttatttcata tcttaaaatt aagttgtatg aggataaaat
     8581 taatttagga ttgataaata attcagataa gtttataaat attactaaag atgagaactt
     8641 cctaaaacat gttcaaaatt atattatcag taatgaaaaa gaaaactgtg taatggcttg
     8701 ttttgattta tgtagactca aacttataaa tgatatatat ggttatgagc taggggatga
     8761 agtgttaaat aatattttaa gtaacttaaa aaattatttt ggaaaagaag ctatttatgg
     8821 aaagttaaaa agtgatgttt ttgtattaat aataaagctt gaaaataggg aacagaagat
     8881 tccttattta gtaaatctta ttaagaataa aattgtgata ctaagtcaga ttgacagttt
     8941 taaaatggat ataaatattg aggcttctat tggaatttat aaatttaaca ataatgaaat
     9001 tgatataaaa aaagctatag ataatgctga tatggcaagg cttaaatcaa aaggactaaa
     9061 gcacatagag tatgtagaat ttgataatgc tatggaagaa gaaatacaat caattatgaa
     9121 aatagagaga gatttattct tggctattaa aaataaagag tttgtaattc actatcaacc
     9181 taaagttgat tcaagtacag gtaacattat aggttcagag gcattgataa gatggttgca
     9241 cccatcatta ggcatggttg gaccaaataa gtttatacct atagctgaaa aaaatggtct
     9301 gataaacaat attggtagat ggctaattca agaagttttt attaccatta acaagtggat
     9361 ttcagaaggt ataaatgtct taccagtctc aataaatctt tctagagtag aactatataa
     9421 aaatgattta gttgattttt ttaaactgat gtttaataca tataatattc caaaagaact
     9481 tatagaatta gaaattacag aaacgacagc actaagagat gtaaaattta taagtgaaag
     9541 gctttatgag attaaatctt taggtatgaa tatatcatta gatgattttg gagtaggtaa
     9601 ttcaaatttt atcaacctta aggggatacc actagatatt ataaaaattg ataggtcact
     9661 tatattggat atagttacaa atacaaaaac aaagtttatg gtgaaagcaa ttgttaacct
     9721 ttcacatgat ttaaacgtaa ctgttatatg tgaaggggta gaggatatgc atcaggtcaa
     9781 agtcttaagt gaactgggat gtaatttcat acaaggttat gtatttttta aacctttgga
     9841 tgagatgaat tataaaaaac ttcttactga cggaagttat ataaatctga aagatactct
     9901 tttagataaa tgcatgacag ttgaggagga gtaaggatga tttcagaaga tgtagattat
     9961 tcaataagaa tcataagagc tatatttaat aatggagaag ataaaccaat agatgctaaa
    10021 atgatattaa aaagtgaaga tataccaata ctaacactat acagactgct tagaaagctt
    10081 tgtgcaaaca atattcttac ttttaacaaa gaagatggca agacatacat gttaaatgtg
    10141 tcattggata caataagtct aagaactatt gtagaagtaa ttgatggaaa aatatatata
    10201 aataaatgta cgagatatgt atctaattgc agtaattgct taaaaggttg taaagtaaat
    10261 cgtgaattgc tagaaataga aaaagaaatt tataaacttt tatcgagaaa atctataaga
    10321 caaatactag aataaaaaat tttcagctat gataataatt ggaggttttc ggttatggat
    10381 gatatatctc aagataattt tctactgtct aaagagtatg agaatagctt agatgtagat
    10441 actaaaaaag caagtggaat atattatact cctaaaataa ttgtagatta tattgtgaaa
    10501 aaaactttaa aaaatcacga tataattaaa aatccatatc cacgaatact tgatatttcc
    10561 tgtgggtgtg gaaattttct tttagaagta tatgatattt tatatgactt atttgaggaa
    10621 aatatatatg aattgaaaaa gaagtatgat gaaaattatt ggactgttga taacatacac
    10681 cgtcatatat taaactattg tatatatggt gctgatattg atgaaaaagc tataagtata
    10741 ttaaaagata gcttaacaaa taaaaaggta gtaaatgatt tagatgaatc tgatataaag
    10801 attaatctat tttgttgtga tagtcttaaa aagaaatggc gatataagtt tgattacata
    10861 gtgggtaatc caccctatat tggtcataaa aaacttgaaa agaaatataa aaaatttctc
    10921 ctagaaaaat atagtgaagt ttataaggat aaagctgact tatatttttg tttttataaa
    10981 aagattatag atattttaaa gcaaggaggg attggcagtg ttataactcc aagatatttt
    11041 ttagaaagtc tatcaggtaa agatttaaga gaatatatta aatcaaatgt caatgttcaa
    11101 gaaatagtgg attttttagg tgcaaatata tttaaaaata taggggtatc tagttgtatt
    11161 ctaacttttg ataagaaaaa aacaaaagag acatatatag atgtatttaa aatcaaaaat
    11221 gaggatatat gtataaataa atttgaaaca ttggaagaac ttctaaagag tagcaaattt
    11281 gaacatttta atataaatca gagattgcta tctgacgaat ggatacttgt aaataaagat
    11341 gatgaaactt tttataataa gatacaagaa aaatgtaaat atagcttgga agatatagca
    11401 ataagttttc aaggaataat tacagggtgt gataaagcgt ttattttaag taaagatgat
    11461 gtaaagttga accttgtaga tgataaattt ttaaaatgtt ggataaaaag taagaatata
    11521 aataaatata tagtagataa aagtgaatat aggctaatat actcaaatga tatagataat
    11581 gagaatacaa ataaaaggat actagatgaa attatagggc tatataaaac aaaattagaa
    11641 aacagaagag aatgtaaaag tggaataagg aaatggtatg aacttcaatg gggtagagaa
    11701 aagcttttct ttgaaagaaa aaagataatg tatccttata aatcaaatga aaatagattt
    11761 gctatagatt atgataataa tttttcaagt gcagacgtat attctttttt tataaaagaa
    11821 gagtatttag ataaattttc atatgaatat ctagttggaa ttttaaattc cagtgtttat
    11881 gataaatact ttaaaataac tgctaaaaaa atgagtaaaa atatatatga ttactatcct
    11941 aataaggtta tgaagataag gatttttagg gataataatt atgaagagat agagaattta
    12001 tcaaagcaaa taatatcaat tttattaaat aagtctatag ataaaggaaa agtagaaaaa
    12061 ttgcagataa aaatggataa cttgataatg gattctttag gtatataagc agttgtaagt
    12121 attaaaaaat tataatcttt taattgttta tttaaattta gaaataaaaa gttagccata
    12181 taaaaaatat taaaaatata tacttttcaa tatttttttg ataaaataag aatgaatttg
    12241 tgcaatttta tttttctcca ataactaaaa atagtttttt gaattaatat ttgcttaaga
    12301 ataaaaatca gaaacgtttt tgatatagta ggtgataata tgaaaggatt agttttagaa
    12361 ggtggaggaa caaaaggagc ctaccaaata ggtgcatata aagctttaag agagcttggt
    12421 atagagtttc aaggagtaac tggcacatca attggtgctt taaatggagc atatataatt
    12481 caaaatgata ttgaagtaat ggaagatatt tggttaaatt atgactatac tcattttatg
    12541 aatattgata gagaaaccta tgagaggttt aaaaatgtag atttcactac taaaaatctc
    12601 aatgcagtgt ttgagcttat aaataaagct agaaaaaatg aaggtataga tataagccca
    12661 ttaagacaat taatggagac aacattaaat gaagacataa taaggaaatc aaaaaaagac
    12721 tttggacttg ttacggtctg gtgggataaa aaaattaatc catatccatt gtatatcgaa
    12781 gatataccta aaggaagatt gattgattat ttaattgcta gttccagttt gcctattttt
    12841 caaacgaaca tgatggatga taagttgtat cttgatggaa tgttttgtga taacatgcca
    12901 ataggactct tggaagacaa aggttatgaa gatgttgttg taattaggtt gattgatgat
    12961 tttattggga aaatgaatct aaatcgacat caaaatataa atataaaaac tataatacca
    13021 tcagaatatc taggaggttc tcttaataaa gatagagata atgtagaaaa aaacatccga
    13081 ctaggttact tagatactat gaaagcatat gaaagatgca ctggaattaa gtattatttt
    13141 gatgtagagt ataaatataa tgaagaatat tgttttgata aatttagaag tatgagcaat
    13201 gaaacaatac aatatatctg taatttattg aatataaaaa gagatgcttc acttagaact
    13261 gttatggaaa gtgtaatacc aaagcttggt gaggtgcttg gattgccaaa ggatttttca
    13321 tataaagatt tgttttactg tatatatgaa aagaaacttg aagaaaataa tataaacagg
    13381 ataaaattat atgattttaa caaggttata gatgttgtaa atacaaatat tgattctaat
    13441 agaaatttag cttctaattt tgaagttaga cctaaaatta gtattaaaaa gaacaaaaac
    13501 acaaaattat tgacaaattg tattataaaa gacataaaaa atcaaagtta aataaaaaac
    13561 aaaaaaaaat ttgtaattat actcgaaaaa agtgtatatt agtagtataa tatttttgta
    13621 gcactagtgg gaacaaaaaa tgaccatagg acaaaaaagt accacttagt accattaaag
    13681 taaaaaacag gaaaacaatt ttaaaaaata taaaatgtaa agtttatagg aggatttaaa
    13741 aaaatggaaa aagtagttag tatcaaaaat gcaagtggat tacatgctag accagcagga
    13801 atgtttgtta agaaggcagc tgaatttaaa tcaacagtag aggttgtagc aaaagacaaa
    13861 actgtaaatg caaaatcaat aatgggaata atgagtttag gtttagctca aggagaagaa
    13921 ttaaagttag ttgtaaatgg agaagatgaa gaagtagctt tagctgcaat ggttgaatta
    13981 gttgaaagtg gatttggaga atagatataa taatttttta aaaatttgat atttagaatc
    14041 taatattact taaagtaaag taacagagaa gtattaaaca tatacagacc aacatttgta
    14101 gatatttaat ttctttgtta ctttcatgtt tatttaatca gaagattaag actattgtta
    14161 attgggtata aaaatataaa tattttagaa aatgaaataa gatattttta atcataaatt
    14221 cagagtaaaa atacagtaac aaatagttta taaaatttta taaaaaatta taagtaaata
    14281 aaactataaa aatgtaaatt atttatataa atttataaag tttatgtgct agatgatatt
    14341 ttttaggagg tagagttatg gcttacaaag gaataggtgc ttctcctggt gttgctttag
    14401 ggaaagcgtt agtagttgaa catagtgaat tagtaattga aaaaaaatct atagataatg
    14461 ttgaagctga aattgctaaa ttagaagatg cagttgcagt ttctaaagaa gaattagtaa
    14521 aagttaagga aaaggcttct gaagaattag gagcagagga agcagaaata tttgaagcac
    14581 atttattagt tttagaagac ccagagttaa ttggttctgc aatagataaa attaaaactg
    14641 aaagtgtaaa tgcagagtat gctttaaatg aaataaaaga gatgtttgtt agcatgtttg
    14701 agtctatgga caatgagtac atgaaagaaa gagcggcaga tataaaagat gttacaaata
    14761 gaatacttag acatatatta ggaattaaag ttgttgattt atctgcttta agcgaagaag
    14821 ttgtattaat agcacatgat ttaactccat cagacacagc aactatgaat aaaaaaatgg
    14881 ttttaggatt tttaactgat ataggtggaa gaacttctca tactgctatc atgtctagaa
    14941 ctttagaaat agctgcaatc gttggattaa atgatataac ttcaaaagtt aaggatggag
    15001 attttgtggt tttcaatgga gacacaggtg aagtaatcgt gaatcctgat gaagagacta
    15061 taaataaata tactgaatta aaagctaagt atgaagatga gagaaaagca ttacagctat
    15121 taaaaggaaa accatctgta actttagacg gaaaacatgt ggaattagct ggtaatatag
    15181 gaacacctaa cgatatagaa ggtctaataa agaatgatgc tgaaggtgtt ggtctttata
    15241 gaacagaatt tttatacatg gatagagatt ctttcccaac agaagaaata caatatgaag
    15301 catacaaagc tgtattagaa ggaatggatg gaaagcctat agttataaga acattggata
    15361 taggtggaga taaagaatta agttatttaa gtatggaacc tgaaatgaat ccattcttag
    15421 gttacagagc aataagactt tgcctagata gaaaagatat atttaagact caattaagag
    15481 ctttatatag agctagtgtt catggaagat taagaatcat gttcccaatg atttcatctt
    15541 tagaagagtt gttacaagct aaagaagttg taaaagaagt tttagcagaa cttgattctg
    15601 aaggtgtggc ttatgctaaa gatgtagaaa ttggtatgat gatagaagtt ccatctgctg
    15661 ctgtaatatc tgatgtattg gctaaacacg tagatttctt ctctatagga actaatgatt
    15721 taattcaata cacttgtgca gttgatagaa tgaatcaaaa gataagctat ctttacaatc
    15781 aatttaatcc agctgtttta agacttataa agactgttat agacaatgca cataaagaag
    15841 gcaaatgggc aggtatgtgt ggagaatctg ctggagacca aaaaatgata ccaatacttt
    15901 taggtatggg attagacgag ttctctatgt ctccaatatc aatacttcct gctagaaagt
    15961 taataacttc agttaaagag tcagatatga aaaaattagc tgatgatgta cttaatatgg
    16021 gtactgctga agaaataaaa agctatatag aaaagacttt taatatataa ttaatgttat
    16081 atagttacta aaattcattt ttctaaataa caaaattata ttttacgaat aa
//
LOCUS       DAMKMI010000091        16011 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMKMI010000091 DAMKMI010000000
VERSION     DAMKMI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16011
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.094"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(138..1328)
                     /locus_tag="PC429_002559"
     CDS             complement(138..1328)
                     /locus_tag="PC429_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF3848058.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1588..2019)
                     /gene="dut"
                     /locus_tag="PC429_002560"
     CDS             complement(1588..2019)
                     /gene="dut"
                     /locus_tag="PC429_002560"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF3848059.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2132..4360
                     /locus_tag="PC429_002561"
     CDS             2132..4360
                     /locus_tag="PC429_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF3848060.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4471..4956)
                     /locus_tag="PC429_002562"
     CDS             complement(4471..4956)
                     /locus_tag="PC429_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3848061.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5208..6431
                     /locus_tag="PC429_002563"
     CDS             5208..6431
                     /locus_tag="PC429_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF3848062.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6435..9569
                     /locus_tag="PC429_002564"
     CDS             6435..9569
                     /locus_tag="PC429_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF3848063.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9603..10901
                     /locus_tag="PC429_002565"
     CDS             9603..10901
                     /locus_tag="PC429_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF3848064.1"
                     /translation="MSKIVAISCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11190..11435)
                     /locus_tag="PC429_002566"
     CDS             complement(11190..11435)
                     /locus_tag="PC429_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3848065.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11649..14276)
                     /locus_tag="PC429_002567"
     CDS             complement(11649..14276)
                     /locus_tag="PC429_002567"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF3848066.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14337..15179)
                     /locus_tag="PC429_002568"
     CDS             complement(14337..15179)
                     /locus_tag="PC429_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF3848067.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15186..15824)
                     /locus_tag="PC429_002569"
     CDS             complement(15186..15824)
                     /locus_tag="PC429_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848068.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
BASE COUNT         5616 a         2540 c         2299 g         5556 t
ORIGIN      
        1 agagaaaata aataaaaagc aggtatcaat caaatagtaa catgtattat actatacatt
       61 actatcaata ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg
      121 ttttataata aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag
      181 ctactattta tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata
      241 tctccatttc ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta
      301 tcaaagaata ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta
      361 ctttggtctc tagatactct tggtatacta actcctacag attttttcat aacgtactgc
      421 gtgaatccag aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca
      481 agtagtgtct ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa
      541 taagatggaa ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat
      601 ccagtttgat tgccataact tatttttgtc catcctccac tttcagaaat acttccaacc
      661 tcagtaccat aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg
      721 ttcaatgact ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca
      781 gcattttcat ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct
      841 actctaccat tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca
      901 gtcccatatc ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt
      961 aatccttttg ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta
     1021 cttccaaggc tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat
     1081 ttaactttta cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt
     1141 ttccccattg attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt
     1201 attgttctgc tttctatact cacactctct acttcagact cattagcata tgctaaattc
     1261 cccatagtag gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta
     1321 aaattcataa ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat
     1381 aaaagaaata attgacacta tcaaaatatc aattatttct tttatattaa tattattaca
     1441 aatttgtaac tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt
     1501 taatctttaa tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc
     1561 tatatttttt aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc
     1621 tctttcggag tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac
     1681 tatcatttga gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat
     1741 taatataatt ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac
     1801 agtaacagaa tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt
     1861 aggaagctct atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac
     1921 ctcttctata gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat
     1981 agcatcatca tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt
     2041 ctataaatat tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata
     2101 taattattag tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc
     2161 tgtggcaaaa actatagcat catttttagg tgcaaagacc agacaagatg gttactttga
     2221 aggaaatgat tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa
     2281 ggattatgat aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc
     2341 tgaggataag tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa
     2401 aaagcttttg aatagaaaag atgtagaata tgtaataaat gccactgata atgaccgaga
     2461 aggagaactt atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag
     2521 aatcttggta aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga
     2581 tgaagatgaa atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt
     2641 gattggaata aatttcactt ctgttgctac attgaaatac ggaaatggga aactattaaa
     2701 cataggtagg gtaattcttc caacagttaa attagtctat gatagagata tggaaatact
     2761 aaactttgtt cctaaaactt attatgaaat agaagggcat tttaaagctg aagctggtga
     2821 atataaaggt aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa
     2881 taaaattata gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat
     2941 gtcaaaggaa tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc
     3001 taagtattcc aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg
     3061 taatggtaag ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag
     3121 tttggcttca aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa
     3181 taaaattaaa ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag
     3241 tgctataatt ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta
     3301 tgatgccata aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac
     3361 agaaattaag actgaagtag ataattgtac tttcttaaca aaaggtaagg tgcttaaatc
     3421 aaaaggatac cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa
     3481 taaagatgat gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc
     3541 taaaccgtat actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc
     3601 tgaagaagat acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga
     3661 tgtgttaaag aagataagtc aagtgggcta tgtaaagaaa aaaggtaaat cttattctat
     3721 aacagacctt gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga
     3781 ttatactgga aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa
     3841 agaatatctt actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga
     3901 tgcttcaaaa aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac
     3961 taaaacaaaa gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat
     4021 atcaaaggat tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg
     4081 caaatgcccc atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa
     4141 tcataaagaa tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa
     4201 gaaagttaac aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc
     4261 tttaactgct aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga
     4321 tactggctat ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat
     4381 agcagtagta taataatcta tattatatta ctgctatttc ttttcttatg aagtttcaac
     4441 ttttttaata gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa
     4501 ctttaatgta tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt
     4561 tactagagat tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt
     4621 taattcaacc ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg
     4681 ttccactaat ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc
     4741 tatttgagaa attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga
     4801 tggagccatt ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt
     4861 ttttaacaaa tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa
     4921 agagtccgct attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat
     4981 aatataataa aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa
     5041 acaagaaaaa gttattgact cataataatt ataaatttat aatcaaatta gagaattata
     5101 aattgccaat aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac
     5161 tttaaattct acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt
     5221 taatagtttt cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acagaagaat
     5281 ctgaacctga gaaaccaata gcagtttctg ttcaaaaagc aataggtgga gaaattgaaa
     5341 acacaaattc tttctcagga actacaaagg ttaaagatga aacagctgtt actgcacaga
     5401 ctgttggcac agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg
     5461 aattattgag tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac
     5521 tagatttagc aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa
     5581 atcaggcaaa aacagctctt gacaatgcaa agattcaata tgaggagtct caaagaaatt
     5641 atgacaataa taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa
     5701 tagaattttc tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata
     5761 cagcaacttc aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag
     5821 ctcaagtatc atataattta gcaatgagta atttagacaa attaacatta acatctccag
     5881 tagatggtac aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac
     5941 cagcttttat tatttctaat cctaatatac tagaagttga tttaaatgta gcagaatcag
     6001 atataggtaa atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt
     6061 taggaaaaat agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta
     6121 aagtacttgt aaataataca aacaacaaat ttaaggcagg tatgtctgct caagtaaact
     6181 taagtgttga aaaagaaaat ggtgctgtta cagtacctaa aaaagcaata tttgaagaaa
     6241 atggtaaaaa atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc
     6301 aaactggaat tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata
     6361 cagtgattat cggagggata agtcttattt ctgacggaac taagatattt cctgtagaaa
     6421 aggagaagta gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa
     6481 tggtcttttt agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc
     6541 taatgcctga tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggtc
     6601 ctcaagacgt tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa
     6661 atgtaaaatc tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg
     6721 aatttggaac taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac
     6781 agaccagcct cccagatgat gcagctaaac caacagtttt aaagctggat atgaatgcac
     6841 aggctatagg tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa
     6901 aatacgctga agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag
     6961 accttaaagg tggagaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa
     7021 attatggagt tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc
     7081 cttatggttc aattactcaa ggtgaggata aaatagtttt aagagcgatt gataaattag
     7141 aatccctaga tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat
     7201 tagataatct ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca
     7261 atggaaaaga cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag
     7321 ttatgcagag tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa
     7381 ttaaaattgc taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca
     7441 acttgcttat aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa
     7501 gagcttcact agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc
     7561 tctactttac tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg
     7621 gtatggttgt tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg
     7681 gtagattgag tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac
     7741 ttgcatcaac attgaccaca atagcaatat tcttacctat catgtttaca gaaggtatag
     7801 ctaaaataac ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta
     7861 taggtgcaat tacactcgtt cctagtgtat ttgcaaaatt aagcagagga aaaaatagtc
     7921 aaaagatgca agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat
     7981 tattaagtgt atgtttgaga catcaacgct tagttgtaat atcaagttta cttatttttg
     8041 tagcttcctt atttggaatg ggatttatag gtatggactt tatgcctgca gcagacaagg
     8101 gtgaattatc tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact
     8161 atgtatctat gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa
     8221 ctttgacatc agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa
     8281 aaaatagaaa aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg
     8341 taccagattg taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag
     8401 cagacttcac attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag
     8461 aagctgaaaa agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag
     8521 atacaacaca agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa
     8581 ataccgctga aattgcaggc atgttacgta cagcaataaa tggtgactcc gtaaccactg
     8641 ctactattga tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata
     8701 gcttagaaga tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg
     8761 gtgccttttc agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag
     8821 atttttctgt aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac
     8881 tagcactaga aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg
     8941 gtgaaacaga aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta
     9001 tagcattagt ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa
     9061 tcatgttctg tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc
     9121 aaagtttaag tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata
     9181 atggtatagt tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt
     9241 taattggttt ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag
     9301 caacaacaat tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta
     9361 tgcaaccact agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg
     9421 tgcttatacc tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa
     9481 tgcctaaaat atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa
     9541 agaaaccaaa attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa
     9601 aaatgagcaa aatagttgca attagctgtg gtgttggtat aataactgcc aatttaatcc
     9661 cagtttacgc agataataat acttcttcta taaacaaagt tgataattct attttaagtg
     9721 actcaaataa aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg
     9781 cacttaaaaa tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa
     9841 agttagaagt acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg
     9901 accaaattgg acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa
     9961 tagcaaatga tattactaat aaatttaacg atttagtttc aagagaaaat gagctagata
    10021 aaataaaaaa caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag
    10081 atttagggtt agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac
    10141 aaaatactca aaaagctaaa cttcaagaac taaaaaacaa tcaagactat tttaagttat
    10201 taactaatat agatttaaat aattatcaat tggataaaga atatagattt gaatctttta
    10261 gagtaggtgg ctcagttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg
    10321 accaattgat tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag
    10381 atttaccaga tagacctaac cttacaagac cagttgctcc tacaaaacca gagcaaggta
    10441 acctaactga tgatgaatac aaggttctta tggataagta tgaaaaagat tatgatagat
    10501 ataaaaaaga agttgaagca tacaatcttg aaagacagac ttatgctgct ggccttacta
    10561 cttatgcaaa ttatcttcaa caaaaattta atactgaaaa tggtctagta acattagaag
    10621 acagtaaaaa agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag
    10681 aagatacaat tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata
    10741 ctaaattaag atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa
    10801 gtaatgaaga tgctaaaaat agctatgaca ctcttattgt gaattataac tcactaaaaa
    10861 atggtataga aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat
    10921 tactttaaca tacgtttgat taaaaaatag ttatgcatac tcgtggcttt agtcatggaa
    10981 gattcagaag gcaaataata gaaatagatt agtaaaaata tatttactta taaaactgta
    11041 tacttttata gaggctatct caaaatagag ataagcctct tttttattgt ttaaattata
    11101 aaaatatatt tgattccata caaaaagagt gccttctgaa ctaaaaagtt cattttgaga
    11161 caccctcaaa agcttaactc taaatttaat tattttttat caaatgtttc atctatttta
    11221 catctcacca tgttaacaaa ttcagaaata ttatatcctc taagtttgca tccacattta
    11281 ggacaataat ccagtttcct agataccaaa gccctacaac taggttctgg acatcttatt
    11341 tttagactct catctgctac tttaaatcca catcgtgggc atatactacc tggacgtgat
    11401 gatacttcct ttccacattt aggacacttt gtcaacttta tcccctccag taaactcaat
    11461 ttaattgata gttgaaattt ctctctacaa taatatatgc aatataatta aatacgtttc
    11521 acttacaact atataagtta aatttttata ataaaaatcc cctatattca atttacaaat
    11581 acttaatttg atacattgaa caatagggga ttttattttt atttagttac atattttgca
    11641 gtctattatt atctaggatt ttttatagat gcttgagctg ctgcaagtct agcaattggc
    11701 actctaaatg gtgaacaaga tacatagtta agtcctacac tataacagaa ctctactgaa
    11761 gatggttctc ctccatgctc tccacatatt cctagtttta attctggttt aacttctcta
    11821 cctaatttag ctcccatctt aactaactta ccaacaccat tttggtctag tacttggaat
    11881 gggtcttttt ctaatatttc ttcatctaca tattgaccta agaatttacc agcatcatct
    11941 cttgaatatc caaatgccat ttgagttaag tcattagtac caaaactgaa gaagtctgct
    12001 tcttgtgcta tttcatcagc agttaaacaa gctctaggta tttctatcat agtacctaca
    12061 gtataaggaa cttctatacc ttcttctttt attatagcgt caacagtttc tactatcatt
    12121 tttcttatta tttttaactc atttacttct ccaactaatg gaaccattat ctctggagaa
    12181 actttaacac ctgctttgat tgcttctata gcaccttgta ttatagcttt agattgcatt
    12241 acacatattt caggataagt tattgcaagt ctacaacctc tgtgtccaag cattgggtta
    12301 aactcatcta aatcaactat tctcttctta atatcactta cttttattcc catagatttt
    12361 gaaagttctt ctatagtctc atcatcatga ggcaaaaatt catgtagtgg tgggtctagt
    12421 aatcttatat taactggttt tccatccata actttaaata actctacaaa atcctctctt
    12481 tgcattggta atattttttc aagagctttt tctctttgtt ctactgtatt agataatatc
    12541 atttctctaa ctgctggcaa tctagcttca tcaaagaaca tatgctcagt tctacataga
    12601 cctatacctt ctgcaccaaa ctctatagct gcacgagcat ctctaggatt atctgcatta
    12661 gttctaacca tcatacattt gtgtttgtct acccagttca ttaatttttc aaaatttcca
    12721 gttaaagata cttctgtttt cttaacatct cctaggtaca catttcctgt agaaccatct
    12781 atagaaatgt attctccttc ttttataact acatcatcaa gtgctcttat ttctttatta
    12841 aactcatcaa cttttatttc tccacatcca gcaacacaac atttacccat acctctagca
    12901 acaactgctg catgtgacgt cattccacct ctagctgtaa ggattccttc tgctttaacc
    12961 attccttcta tatcttctgg agatgtctct agtctaacta aaactacttt ttctcctttt
    13021 tcatttgctt ttactacatc atcagcattg aaataaactt taccacttgc tgcacctggt
    13081 gatgctggta aaccttttgc tataaccttg gcttctttta atgccttatc ttcaaatttt
    13141 ggatgtaata attggtctaa ttgattaggc tcaattctca tgatagcctc tttttcatca
    13201 attattccag cttcaactaa atcaaccgct acatttattg ctgctgctgc tgttctctta
    13261 ccatttctag tttgtagtat atataatctt tcattttcta tagtaaactc tatatcttgc
    13321 atatctttat aatgaccttc aagtatatga gtaattttta caaattcatc gaatacagct
    13381 ggcattacat ctgcaagagt tgatatattt tgaggagttc ttatacctgc aacaacgtct
    13441 tcaccttgtg cattcattaa aaattcacca aataatttat tttctcctgt agctggattt
    13501 ctagtaaatg caacacctgt accactagtt tctcccatat taccaaatac cattgattga
    13561 atatttactg ctgttcctaa gttatgagct atatcattca atttacggta tacgattgca
    13621 cgaggattat tccatgatct aaatacagcc tctattgcaa gcattaattg ctcttttggg
    13681 tcttgtggga aatcattttt tatttctttt ttataaattg ctttaaattc ttttactatt
    13741 tcttttaaat catcagttgt aagttctgta tcaactgtgt atccctttgc ttcttttact
    13801 ctgtctaata cattttcaaa tttatatttt ggaacttcca tagcaacatc agaaaacatt
    13861 tgtatgaatc ttctgtaact atcatatgca aatctttcat tttgtgtagc ctctgctaag
    13921 ccaacaacac tattgtcatt aagacctagg ttaagaattg tatccatcat tcctggcata
    13981 gagaatactg caccagaacg aactgatact aataatggat tcttattgca tcctaatgtt
    14041 ttatttaagt ctttttctaa agtagctaaa tgctcttcta tttcttttat tatctctgct
    14101 cttatacttt cattgttaac ataataatca ttacatgctt ctgttgttat agtgaatcca
    14161 ggagggacag gtaagcctat cttagtcatt tctgctaaat tggcaccttt acctcctagt
    14221 aaagatttca tgtctttgct tccttcacca aaactataaa catacttagt ttccattgtt
    14281 aaatccccca ttaatctttt tattttaatt tatatcattt ttattaatag acattattaa
    14341 atttctattt ctttgtatat ctttagacca ttttctttca ttatgtctaa aattatacct
    14401 gctgtttctt ctattgcctt atttgaaacg tttattactg ggcatccaat tcttttcatt
    14461 atctcttctg aataatctaa ctcctgaagt attctttcta aattagcgta gtttgcttta
    14521 ctggacaatc ctaaagcttt taatctttgg gttcttatct cattcagctt ttcaggtgaa
    14581 ttagtaagac ctattatttt tttagtatct atctcaaaca cttctttagg aattggtatt
    14641 tctggaacta atggaacatt agctactttt atatttttat tagctaaata catactcaaa
    14701 ggagttttag atgttcttga tattcccact aaaattatgt ctgcttgaag aacacctcta
    14761 gggtcttttc catcatcata tttcactgca aattctatag cttcaactct cttaaagtaa
    14821 gattcatcta attttctaat tataccagct tctcttttag gttttcttcc tgttctcttg
    14881 cttatttctc ttagtatatc tgtcattaaa tctatacaac taagaccttc tctttcgcaa
    14941 tatctttcaa caatactgca taattcttca tcaactaatg tatatacaat taaagcgttt
    15001 tcagcttttg cattctccaa agtttcaagt agtactcctt tattaatcat gtatggaaat
    15061 ctttttattt cataagtttc attcacataa aactgagaca tacatgcctt tgcaacttgt
    15121 tgtgcagtct cccctactga atctgatact gcatatataa ttaggttttt caccagcata
    15181 cctccttaat tgtcaactat ttctaagaat agcttagtta tgttggtttt tgatattctc
    15241 ccaacaactc tcatatgatt tttgtcttct cccttatatt caacaatcgg aatcgaatct
    15301 acttcatgtt ctattatttt tcttgaagct aataaaacac ttgctccttt agttaaagtg
    15361 actacatttg gagttcttgt cattatcata cctattggca ttttatttat atcagaacct
    15421 cctatagttg cttttagcaa gtcttttctg gaaacaactc cacataactc ttcattctca
    15481 tctataataa ctatactccc tacatcagat aaaaacatag tgactattac atcatatata
    15541 ctttcatcct tcttagctag tacaggcatg ctcattatat cctctactgt tttttctttt
    15601 acacttttac caattaaatt tatttcacta actcctgaat aaaaatatcc aacttttggt
    15661 cttgcgtcta atattcctgt cattgttaat atagctaaat cagatctaag tgtagcacga
    15721 gtcacattca agctggaggc aatactttca ctcgttattg gttcattttc cttaactata
    15781 tctataattt ttagttgtct ttcattaagc tgaataataa tcaccttctt tcattgtgct
    15841 atacttttta tcttttcgca tcttatatag tatattatat aactatatat accattctgt
    15901 aaacagattt tttattgaat acgatatatt ttttactaag ttctctcgct taaagatatt
    15961 tattatatat taatggggga tatttcttta attaatagaa ctatattagt a
//
LOCUS       DAMKMI010000092        15979 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMKMI010000092 DAMKMI010000000
VERSION     DAMKMI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15979
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.095"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="PC429_002570"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="PC429_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF3848069.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="PC429_002571"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="PC429_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF3848070.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="PC429_002572"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="PC429_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF3848071.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="PC429_002573"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="PC429_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF3848072.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="PC429_002574"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="PC429_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF3848073.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="PC429_002575"
     CDS             2662..3168
                     /locus_tag="PC429_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF3848074.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="PC429_002576"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="PC429_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF3848075.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3983..4771
                     /locus_tag="PC429_002577"
     CDS             3983..4771
                     /locus_tag="PC429_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF3848076.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            5242..8958
                     /locus_tag="PC429_002578"
     CDS             5242..8958
                     /locus_tag="PC429_002578"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF3848077.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIVKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            9000..12485
                     /gene="rpoC"
                     /locus_tag="PC429_002579"
     CDS             9000..12485
                     /gene="rpoC"
                     /locus_tag="PC429_002579"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF3848078.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            12774..13196
                     /gene="rpsL"
                     /locus_tag="PC429_002580"
     CDS             12774..13196
                     /gene="rpsL"
                     /locus_tag="PC429_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF3848079.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            13320..13790
                     /gene="rpsG"
                     /locus_tag="PC429_002581"
     CDS             13320..13790
                     /gene="rpsG"
                     /locus_tag="PC429_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF3848080.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            13839..15905
                     /gene="fusA"
                     /locus_tag="PC429_002582"
     CDS             13839..15905
                     /gene="fusA"
                     /locus_tag="PC429_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF3848081.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         6256 a         1987 c         3251 g         4485 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactag ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgttgtaa tgtctatata taaagtatat aattaaacta aaatgtaaaa ttttacattt
     3841 tagtttaaaa aaatacacga atgttttata aataaaatgt aataaataat taattaacaa
     3901 atataaattt taaaataagt tagatattta gaaaaatcga aatttgtagg atttaaagga
     3961 catattcaag gaggagttaa taatggaaaa attacaagga aaaattgcag tagttactgc
     4021 agcaacaaaa ggtattggat tagcatcagc agagatatta gcaaaaaatg gagcaactgt
     4081 gtacttagca gctcgttcag aagaattagc taatgaagtt ataaataaaa taagtgcaga
     4141 aggtggttgt gctaagtttg tttactttaa tgctcgtgaa gaagagacct ttacctcaat
     4201 gatagaagag gtagttaaaa aagaaggtaa gatagatata cttgtaaaca attttggttc
     4261 aacaaaccct tctcttgaca aagaccttgt aactggggat acagataatt tctttgacac
     4321 agtaaatact aatttaaaaa gtgtgtattt accatgtaaa gcagcaattc ctcatatgat
     4381 aaagaatgga aaaggaagta tagtaaatat atcaagtata ggttcagtat tacctgattt
     4441 atctagaata gcttactgtg tatcaaaagc agcaatcaac tcattaactc aaaatatagc
     4501 tacacaatat gctaaagaca atgttagatg taatgctgta cttccagggc ttattgcaac
     4561 taaagcagca ttagacaata tgtctccaga gttcataaaa gaatttttaa agcatgttcc
     4621 tttaaatcgt ataggggaac cagatgatat agcaaaagct gttctattct atgctagtga
     4681 tgactcatca tttataacag gggatttact tgaagttgca ggaggatttg gtttacctac
     4741 tccacaattt gcagataata tattaggata attaaattac taatatgcaa tgaaagctta
     4801 tgcattagtt atagtagaaa tgatattttt ataaaggcca tagatataat ttatattgga
     4861 aaggtttgag gttcaaaatt ccaagttaaa tttatttatg gtctttattt tgtatatttt
     4921 aataaattgt atatatttgt ataaatgtaa ttatttacaa tcattttaat atggaattat
     4981 aattaaatag gggggatggt acttgtataa aattttttat agaaaattag acctataaat
     5041 gaaaaatatg gattttatat tggaaaattg tacataatac ataatatatg cttaaaataa
     5101 tttacaaaaa atcaaaaata atgcatagtt tacatataag ataaaaaata tattgaaaaa
     5161 tacttgacat tatgctaaaa ttattagatg cgttagaatg aactattgtt ttatttttaa
     5221 ttattgagag gtgaaaagtg aatgccacat cctgtcacga taggtaaaag aactagaatg
     5281 agcttttcta aaattaagga aatagctgat gtgccaaatc ttatagaaat tcaagtagat
     5341 tcctatgagt ggtttttaaa agaaggttta aaagaagtgt ttgatgatat ttcaccaata
     5401 gaagattata ctggtaatct tatattggaa tttgtcgact attctctaga tgataaacca
     5461 aagtacgata tagaagagtg taaagagaga gatgctacat attgtgcacc tctaaaggtt
     5521 aaggtaagac ttataaataa agaaacaggt gagataaaag aacaagaagt gtttatgggt
     5581 gacttccctt taatgacaga aagaggaact ttcgttataa atggagcaga aagagttata
     5641 gtaagtcaat tagttagatc tcctggtgtt tactatgcgg aagaaagaga taaaacaggt
     5701 aagagattga tttcatctac tgtaattcct aatagaggag catggttaga atatgaaaca
     5761 gattctaatg atgtaatatc tgtaagagtt gatagaacaa gaaaacaacc agttacagtt
     5821 ttacttagag ctttaggaat aggaacagat gcagagataa ttgacctttt aggagaagat
     5881 gagagattat ctgctacatt agaaaaagat aatactaaga cagtagaaga aggtcttgta
     5941 gagatataca aaaaattaag accaggtgaa cctcctactg tagaaagtgc atcatcttta
     6001 ttaaatgctt tattctttga cccaaaaaga tatgacctag caaaagttgg tagatataaa
     6061 ttcaataaga aacttgcttt atgttacaga ataatgaata aaatttcagc agaagacata
     6121 ataaatccag aaactggaga agtatttgta aaagctggag aaaaaataag ctatgatttg
     6181 gctaaagcca tacaaaatgc aggtataaat gtagtaaatc tactaatgga tgatgataaa
     6241 aaagttagag ttataggaaa taattttgtg gatattaagt ctcacattga ttttgacata
     6301 gatgatttaa acataaaaga aaaagtacat tatccaactt taaaagaaat tttagatgga
     6361 tatagtgatg aagaagaaat aaaagaggct ataaaatcaa gaattaaaga acttatacca
     6421 aaacatatat tattagatga tataatagct tcaataagtt atgagttcaa tatattctat
     6481 aatataggaa atattgatga tatagatcac ctaggaaaca gaagaataag atctgtaggt
     6541 gaattgttac aaaatcaagt tagaattggt ctttcaagaa tggaaagagt tataaaagaa
     6601 agaatgacgg ttcaagatat ggaagctata acgcctcaag cgttagttaa cataagacca
     6661 gtttcagctg caataaaaga attttttggt agttctcagt tatctcagtt catggatcaa
     6721 acaaatcctc tatctgagtt aacgcataag agaagattat cagcccttgg gcctggaggt
     6781 ctttcaagag aaagagctgg attcgaagtg cgtgacgttc accattcaca ctatggtaga
     6841 atgtgtccaa tagagactcc agagggacca aatatcggtc ttataaactc tctaggaact
     6901 tatgcaaaaa taaatgaatt tggatttata gaatcaccat atagaaaatt tgataaagaa
     6961 acatcaacag ttactgatga aatacattat ttaactgctg acgaagaaga tttattcgta
     7021 agagctcagg caaatgagcc attaacagaa gatggtaaat tcgtaaacca tagagttgtt
     7081 tgtagaactg taaatggtgc tgttgaaatg gttcctgaaa gtagagtaga ttacatggat
     7141 atatctccta agcaggttgt atctgttgcc actgctatga tacctttctt agaaaatgat
     7201 gatgccaacc gtgcccttat gggagcgaac atgcagcgtc aggcagtgcc tctagttaga
     7261 agagaagcac caattatagg aactggtata gaatacagag cagcaaaaga ctctggagca
     7321 gttgttgttg ctagaaattc tggtatagca gaaagagtaa cagcagatga aataattata
     7381 aaaagagaag atggaaacag agatagatat aatctactta agtttaaacg ttcaaactca
     7441 ggtacttgta taaatcaaac acctataata aataagggtg accagatagt aaaaggtgat
     7501 gttatagcag atggtccagc aactgattta ggagaagttg cacttggaag aaactgtctt
     7561 atagcattca tgacttggga aggttataac tacgaggatg ctatattaat aaatgaaaga
     7621 ttagttaaag aagatagatt atcaacaatt catatagaag aatatgaatg tgaagctaga
     7681 gatacaaaac tagggccaga ggaaataact agagatatac ctaatgttgg agatagtgca
     7741 attaagaact tagatgacag aggtataata agaataggtg cagaagtaga ctcaggtgat
     7801 atattagttg gtaaagtaac tccaaaagga gaaactgaac ttactgctga ggaaagatta
     7861 cttcgtgcaa tatttggaga aaaggctaga gaagttagag atacttcact taaagtgcct
     7921 catggtgaat ctggtataat agttgatgta aaagtattta ctagagaaaa tggagatgat
     7981 ttatctccag gagtaaatga attagtaaga tgctatatag ctaaaaagag aaaaataaaa
     8041 gttggagata aaatggctgg tcgtcatggt aataaagggg ttatctcaag agtattacct
     8101 gaagaagata tgccattcat ggaaaatgga acaccactag acataatact taacccacaa
     8161 ggtataccat cacgtatgaa cattggtcag gttttagaag ttcatttagg acttgctgct
     8221 aagacattag gatggtatgt agcaacatca gtattcgatg gtgctaatga gtatgacata
     8281 atggatgctc ttgaagaagc tgggtatcct agagatggta aattaacttt atatgatggt
     8341 agaacaggtg agtcttttga caacagaata actgttggat atatgtacta cttgaaacta
     8401 catcacttag tagatgaaaa actacatgca agaagtactg gaccatactc attagttact
     8461 caacagccac taggtggtaa agcacaattt ggtggacaaa gatttggaga gatggaggtt
     8521 tgggctctgg aagcatatgg agctgctcac atacttcaag aaatacttac tgttaagtct
     8581 gatgacgttg taggtcgtgt tagaacatat gaagctatag ttaaaggtga aaatatacct
     8641 gaaccaggaa tcccagaatc atttaaggtt cttataaaag aacttcaaag tttatgctta
     8701 gatgtaaaag tattgacaga tgaagaccaa gaaatagaag taagagaatc tgtggatgaa
     8761 gatgatacaa taggtgaatt tgagctagat gttgtaaatc atatgggaga agttgaagaa
     8821 agtaatataa tagaagaaat tgaagatgat tttgcagaaa atgcagaaga tgaggatata
     8881 gagaatttag aagaattcac tgaagatgat ttatttgaag aagaaataga ttttgatagt
     8941 gatgactttg atatgtaaat ttaattaatg taaatctcaa atagaaggga gagaactcct
     9001 tgtttgaatt aaacaatttc gagtcgataa aaatagcatt ggcttctcca gaaaaaataa
     9061 gacaatggtc taggggagaa gttaaaaagc cagaaactat aaattaccgt actttaaaac
     9121 cagaaaaaga tggtcttttc tgtgaaagaa tatttggacc acaaaaagac tgggagtgcc
     9181 actgtggtaa atatagaaga gttagatata aaggtgtagt ttgtgataga tgtggagtag
     9241 aagtaactaa atcaaaagta agaagagaga gaatgggaca tatagagcta gcggctccta
     9301 tgtctcacat ctggtacttc aaaggtatac caagtagaat gggactttta cttgatatgt
     9361 caccaagatc gttagaaaaa atattatact ttgcctcata tgtggtagtt gatccaggag
     9421 agactggatt aaatgaaaaa caattgctta cagaaaaaga atacagaact gctcttgaaa
     9481 agtatggata tacttttact gtaggaatgg gtgctgaagc tgtaaagaca ttactacaaa
     9541 atatagattt agagcaacaa agtaaagacc taagagcgga gttaaaagat agtacagggc
     9601 aaaagaaagt tagaacaata agaagattag aagttgtaga ggcatttaaa aagtctggaa
     9661 ataaaccaga atggatgatt ttagatgcaa taccagtaat accaccagat ttaagaccaa
     9721 tggtacaact tgatggtgga agatttgcga cttcagacct aaatgattta tatagaagag
     9781 ttataaatag aaacaataga cttaaaagat tattagagct tggagctcca gatataattg
     9841 taagaaatga aaaaagaatg cttcaagaag ctgttgatgc attgatagat aatggtagaa
     9901 gaggtagacc tgtaacagga cctggaaata gaccacttaa atctttatca gatatgttaa
     9961 aaggtaagca aggtcgtttc cgtcaaaact tacttggtaa gcgtgttgac tactcaggac
    10021 gttctgttat agttgttgga ccagaactta aattttatca atgtggactt ccaaagaaga
    10081 tggcattaga attattcaag ccatttgtta tggataaatt agttaaagaa ggttatgcac
    10141 ataatataaa aagtgcgaaa tctatagtag aaaaagttaa gccagaagtt tgggatgttt
    10201 tagaagatgt tataaaaagt catccagttc ttcttaaccg tgcaccgact ctacatagat
    10261 taggtataca agcatttgaa ccaatcttag ttgaaggtaa ggctataaaa ctacatcctc
    10321 ttgtatgtac agcttataat gcagactttg atggtgacca aatggcagtt catgtacctt
    10381 tatcagtaga agcacaagca gaagcaagat tcttaatgct ttctgtaaat aatatacttg
    10441 ctcctaaaga tggttcacct ataactactc catctcagga tatggtttta ggttgctatt
    10501 atctaacaat agaagcccaa gatggagcta aaggaacagg tatggtattt aaagacttta
    10561 atgaactatt acttgcttat tacaataaat cagttcatct acatgcatta gtgaaactta
    10621 aggtaacact tgaagatgga agaagttcat tggttgaaag tactgttggt agatttatat
    10681 ttaatgaaaa tatacctcaa gacttaggtt ttgtagatag aaaagaaaat ccatttgcac
    10741 ttgaagttga tttcttagca gataaaaaat ctcttggtaa aataatagat aaatgcttta
    10801 gaaaacacgg aaatacagaa acagctgaat tactagatta tataaaagct ctaggattta
    10861 aatattctac attaggtggc ataacagttg ctgttgatga tatgagtgtc ccagaagaaa
    10921 agaaagtatt tatagctgag gcagaagcta aagttgataa gtatgaaaaa gcatacagaa
    10981 gaggtctaat ctctgatgaa gaaagatatg aaaaagttat agagacatgg acagaaacaa
    11041 ctgataaagt tactgatgct cttatgggtg gactagatag attaaataat atatatataa
    11101 tggcacattc aggagccaga ggttctaaaa accaaattag acagctggca ggtatgcgtg
    11161 gtcttatggc aaatgcatct ggtaaaacag ttgagatacc agttaaatct aatttccgtg
    11221 aaggtttatc agtactagaa tactttacat cttcacatgg tgccagaaaa ggtcttgccg
    11281 atacagctat acgtacagct gaatctggat atttaacaag aagacttgtt gatgtaagtc
    11341 aagatgttat tgtaagggaa atagattgtg gaacagaaga tactacagag atttatgcta
    11401 taaaagaagg aaatgaagtt atagaagaga tatatgatag aattgtagga agatatacta
    11461 tagaccctat attaaatcct gaaactggag aagttatagt tgaggctgat tcaatgatac
    11521 aagaagatga agcagaaact atagtagctt taggaattga aaaaattagg ataagaacag
    11581 ttcttaactg taaaactaat catggagttt gttctaagtg ttatggtaga aacttggcaa
    11641 caggaaaaga agttaatata ggtgaagcag ttggtataat agcagctcaa tctatcggtg
    11701 agccgggtac tcagcttaca atgcgtacat tccatactgg aggagttgca ggagctgata
    11761 taactcaagg tctaccaagg gttgaagaat tatttgaagc tagaaaacca aaaggattag
    11821 ctgtaataac tgaagtatct ggtagagttg aaattgatga aacaggaaag agaaaagaag
    11881 taaatgtaat accagaagaa ggcgagactc aaacatatgt aattccatat ggttctagat
    11941 taaaagttaa gcaaggtcaa atgctagagg ctggagaccc tctaacacaa ggatttataa
    12001 atcctcatga tatagtaaga gtaaatggtg ttaagggagt tcaagaatat atagttaaag
    12061 aagttcaaag agtgtataga cttcaagggg ttgatgttaa tgataagcat atagaagtta
    12121 tagtaagaca aatgctatct aaagtaaaag ttgaagaccc aggagataca gatttattgc
    12181 caggtggata tgaagatgta ttaacattca atgaatgtaa taaagatgct atagataaag
    12241 gtctaagacc agcagttgct aaaagagttt tacttggtat aactaaagca tctcttgcaa
    12301 ctgattcatt cttatcagca gcttctttcc aagaaacaac aagagtatta acagaggcag
    12361 caataaaagg taaagaagac cacttaatag gacttaaaga aaatgttata ttaggtaagt
    12421 taataccagc aggaacagga atgaagaaat atagaaatat agctgttgaa aaaattgaag
    12481 attaatagca tatagagtaa agaaatcttg acaaccatta tttgtggtgt taaaatagta
    12541 aagtgtgttg tttaaaagta cactaagggc ttgaacaagt tcttagtgac ttttagatgg
    12601 caataaatga gaactaaaaa gcatcaagct agaaaagtgc taatatcaca tatttatgtg
    12661 ttatagacat taaattgtaa gtaacgaatg ttacaatcag tcagtatgaa tattcatatt
    12721 gatgtagttg gcggtcactg ataagatcga aaatttggaa ggaggtgcag atgatgccaa
    12781 caattaacca attagttcgt aaaagtagaa aagcgctaga gaagaaatct actgctccag
    12841 cattacaaaa aggttacaac tctttaaata agaaagtaac tgacgctagt gccccacaaa
    12901 aaagaggggt ttgtacttca gtaaaaacag ttactcctaa gaagcctaac tcagctttaa
    12961 gaaaagttgc cagagttaga ttaactaatg gtatagaagt ttctgcttat ataccaggag
    13021 aaggacacaa cttacaagag catagtgttg ttcttataag aggaggaaga gtaaaagacc
    13081 ttccaggggt tagataccat atactaagag gtactctaga tacagcaggt gttgataaga
    13141 gaagacaatc aagatctaaa tacggtgcga agagacctaa agaagctaaa aagtaaaaac
    13201 aattaatgat tgattatagt tcggtgcttt aaatatatat atatttacgg catcacggca
    13261 ttaaatgatt tatgtcgagt acctatgatt taagttaaat aattaaggag ggaagcgaaa
    13321 tgccaagaaa aggtaatatt ccaaaaagag aagttttacc agatcctatg tatgggagta
    13381 aggtagttac taaattaata aataatctaa tggtagacgg aaaaaaagga aagtctcaaa
    13441 gaatagttta tgatgctttc gcaattgttg ctgaaaagac aggagaagaa gctttagaag
    13501 tatttaataa agcaatggat aatataatgc ctgttttaga agtaaaagca agaagagttg
    13561 gtggggcaaa ctaccaagtg ccaatcgaag ttagacctga aagaagacaa acattaggtt
    13621 taagatggct agtaaaatat actagagctc gtggagaaaa aggaatggtt gaaaagctag
    13681 ctaaagagat aatggacgca gctaacaaca ctggagcttc agttaagaaa aaagaagata
    13741 ctcataaaat ggcagaagct aataaagcat ttgctcatta cagatggtag gatatataga
    13801 tatcttataa aattgaggat atcataagga ggataaccat ggctagaaag tttcctttgg
    13861 aaagaactag gaatatagga atcatggctc atatagatgc aggaaaaact actactacag
    13921 aaagaatcct gttttatact gggcaaactc ataaaatagg agaaactcat gaaggagctt
    13981 ctcaaatgga ctggatggag caagaaaagg aaagaggtat aacaataact tctgccgcta
    14041 ctactgcttc atggaaagac catagaataa acataataga tactcctgga cacgtggact
    14101 tcacagttga ggttgaaaga tctctaagag ttcttgacgg ttcagttgct gtattctgtg
    14161 caaaaggtgg ggttgagcct caatcagaaa atgtatggag acaagctgaa acttatggag
    14221 tacctagaat agcttttgta aataaaatgg atatattagg tgcagatttc tacaatgttg
    14281 taagcatgat gaaatctaga ttaaattcta atgcagttcc tatgcaatta ccaataggta
    14341 aagaagattc atttataggt ataatagact tattaaaaat ggatgctgtt atatacaaag
    14401 acgacctagg ggttgaaatg gaagaaacag atataccaga agatatgaaa gaattagctg
    14461 ctgagtggag agaaaaatta gttgaatcag tagctgaaac tgatgaagaa ttaatgatga
    14521 aataccttga aggtgaagaa cttactatag atgaattaaa agttgctata agaaaagcta
    14581 ctatagcttg tgaaatgaac ccagttttct gtggtacagc ttatagaaac aaaggtgttc
    14641 agttagtaat agatgcagtt ttagattact taccagcgcc aactgatata ccagctataa
    14701 aaggtatatt agcagatgga gaagaagctg aaagacattc ttctgatgaa gagccattct
    14761 ctgcattagc attcaaaata atgactgacc cattcgttgg aaaactagcg ttcttcagag
    14821 tttattctgg aacattagaa tcaggttctt atgttttaaa tgcaactaag aataagagag
    14881 aaagaattgg tcgtatatta caaatgcatg ctaatacaag agaagaaata actaaagtgt
    14941 atgctggaga tatagcagca gcagtaggat taaaagatac tactactgga gacacattat
    15001 gtgaccctgc taatccaata atacttgaat ctatggaatt ccctgagcca gttatatctg
    15061 ttgctataga accaagttct aaagcagctc aagaaaaaat gggtatagct cttcaaaaat
    15121 tagctgaaga agacccaact ttcacagtta agactgacca agaaacagga caaactataa
    15181 tatctggtat gggtgaatta cacttagaga taatagtaga tagattatta agagaattta
    15241 aagtagaagc taaagttggt gcaccacaag ttgcatatag agagactata actcaaccag
    15301 ttgatgttga atacaagtac tctaagcaat caggtggtag aggacaatac ggtcacgtta
    15361 agattagagt tgctcctcaa gagccaggtg aaggatacaa attcacaaac aaaactgttg
    15421 gtggatcagt accaaaagaa tatgttggtc cagttgacat gggtatacaa ggagctatgc
    15481 aatctggtat agttgctgga tatccagttg tagacgttgc tgttgagtta tatgatggtt
    15541 cttaccatga ggttgactct tctgagatgg cgtttaagat ggctggttca atggcattca
    15601 aagatgctat gaagaaaggt aatgcagttc ttttagagcc ttacttcaaa gtagaagttg
    15661 ttactccaga agattacatg ggagatgtta tgggtgactt aaactcaaga agaggattaa
    15721 tccaaggtat ggaagctaga agtggtgcac aagttataaa tgcattcgtt ccactttcag
    15781 aaatgtttgg atactcaaca gacctaagat cttcaactca aggtcgtgca acttacacaa
    15841 tgatattcga tcactatgag caagttccag cttcagttgc taagaaaata gctgaaggaa
    15901 aataatttaa aaataattaa taatataaat ataaaaaata atcagctaga tgagagatat
    15961 ctctcatcta gcacaagat
//
LOCUS       DAMKMI010000093        15944 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMKMI010000093 DAMKMI010000000
VERSION     DAMKMI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.096"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            70..666
                     /locus_tag="PC429_002583"
     CDS             70..666
                     /locus_tag="PC429_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF3848082.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(764..1723)
                     /locus_tag="PC429_002584"
     CDS             complement(764..1723)
                     /locus_tag="PC429_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3848083.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(1757..2488)
                     /locus_tag="PC429_002585"
     CDS             complement(1757..2488)
                     /locus_tag="PC429_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848084.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(2481..3251)
                     /locus_tag="PC429_002586"
     CDS             complement(2481..3251)
                     /locus_tag="PC429_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848085.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(3336..4046)
                     /locus_tag="PC429_002587"
     CDS             complement(3336..4046)
                     /locus_tag="PC429_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF3848086.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            complement(4226..4378)
                     /locus_tag="PC429_002588"
     CDS             complement(4226..4378)
                     /locus_tag="PC429_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF3848087.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(4404..6560)
                     /gene="feoB"
                     /locus_tag="PC429_002589"
     CDS             complement(4404..6560)
                     /gene="feoB"
                     /locus_tag="PC429_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF3848088.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            complement(6660..6881)
                     /locus_tag="PC429_002590"
     CDS             complement(6660..6881)
                     /locus_tag="PC429_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3848089.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            complement(6894..7106)
                     /locus_tag="PC429_002591"
     CDS             complement(6894..7106)
                     /locus_tag="PC429_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3848090.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            complement(7427..10324)
                     /locus_tag="PC429_002592"
     CDS             complement(7427..10324)
                     /locus_tag="PC429_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3848091.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLNSGYEYSVLYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            complement(10651..11259)
                     /locus_tag="PC429_002593"
     CDS             complement(10651..11259)
                     /locus_tag="PC429_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF3848092.1"
                     /translation="MVDNLIKYIHKSYVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(11519..12064)
                     /locus_tag="PC429_002594"
     CDS             complement(11519..12064)
                     /locus_tag="PC429_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF3848093.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(12182..14005)
                     /locus_tag="PC429_002595"
     CDS             complement(12182..14005)
                     /locus_tag="PC429_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848094.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(14018..15793)
                     /locus_tag="PC429_002596"
     CDS             complement(14018..15793)
                     /locus_tag="PC429_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848095.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         5293 a         2834 c         1566 g         6251 t
ORIGIN      
        1 tattgattat cattcatagt ataatctaat tacttgatat taattttcat ttgaaggagg
       61 ttatctttta tgtcttgttg taattcacaa ttttgttgta aaaataaaat tgaatcatca
      121 tatataaaat ggcttttaga agttttaggt ccagtctttt taggctctaa acctgctgaa
      181 ataataaata tttcgtttac tgatgtaaat agagaggaaa aaattaatga tatttataaa
      241 tatctatata agtgtaagaa aatagatttt atagttattg ataaagaaaa aaagggatta
      301 aaaatcttat ttgtaaataa aaaagcatta tcagaaaaat taaaatgtaa aaaaacagta
      361 aactttttaa agtttttagg atacaaacaa aatacgaatg ttaatgctta tttagagcat
      421 ttagtagata aacttaagag tgatgttttt cctgatgaaa taggtatttt tttaggatat
      481 cctcttaaag atgtgattgg atttatggga tatagtaatt atgaagtttc tatgattaaa
      541 tattggaaag tttatggtga cacaaaacaa tccgaggaca catattcgaa gtttttactt
      601 caccgtaaga aaatgagaaa attactagac tacataagtg tagataaaat agtaagctgt
      661 ttttaattgt gtttttgtat aataataaaa atacaccctc ttttaccata cagtttccat
      721 aataaataag ggtgtatttt ccactttaat attttaaagt attctaattt tgattgtaca
      781 acatatcttt aatatttatt ttcttttgtt gcattccatt agacatcaaa aattcctgtg
      841 tatcttctaa ttcttttata tccttgtccg ttattttaga actaaagtca taccaagaat
      901 acatttcttt tgtttcatct aaagataaac caacttcttt agatacaaca tccatcactt
      961 catccttgtt ttcattcata tattttaaag tttcttcatg aaccttcata aatcttttta
     1021 ctaattcagg atatttttca gcaaagtcat cacttacagc agtaactatt attccactta
     1081 ctaatccttc tccatttaca atcattttgg cacctgaatt tatcgttttc aatgccactg
     1141 gacctgcaag cattgctaca tcagcacttt tactttctaa tgcagaagca gcactaggaa
     1201 tatccatatt tacatattca acatcatcta tagtatagcc ttctttagca agtgcagcaa
     1261 tcaacagttg atgaagtata gtcccttttg gacctacaat ttttttacct tttaagtctt
     1321 ttatagattt aatattatca ttattagtta taagcatata tccttttggt gaacggctat
     1381 aaatattcgt aattttaaga ccaacaccat ttgatgcagc tattattgca gacgttcctc
     1441 ctaatgcatg taaaaagtct agctcacctg ctgctaatgc ttgagtttgc tctggtcctg
     1501 ttgtcaattc atggaaattg acttttatat tgtccttttt aaattccttt ccaaataaat
     1561 catccttttt ttctaatata ctaggaacat taagtggtga ttttacataa gttaaattta
     1621 tttccttagg taaactagcc tctttttttg caggttcatc tttcttatca tttgttgttg
     1681 aacatcctac agtcccaacc aacaccatta ttaaagatgt cattactgct aaaatctttt
     1741 tgtttctttt aatcaattaa aactccccca attcccttaa tatttctttt ttcaagttta
     1801 tataatagtt tttagttaaa tctctattat attcatcttc gacactaaat tgtttaatcc
     1861 ttttattttt actaaaaaca attattttct ttgctatttc catagcttct tcaatattgt
     1921 gagttacaaa tactacacct tttttagtat ttttatggat atttacaact tctttttgca
     1981 tttttcttct tgtgaagtaa tctagtgctg caaatggttc gtccatcagc aaaatatcgg
     2041 gattgaagga aagtgctctt gctatactta ctctgtgagc cattcctcct gataattcat
     2101 ttggatatga gtttttaaac ttttcaagct tcatcatttt taaatacttg tctaaatcaa
     2161 ctttatttct attatctttt tcagtatgta aaagtatatt ttcacttaca ttcagccatg
     2221 gcataagtct actttcttga aataccattc ctacttttgg agtacacttt ttatcattat
     2281 taaaaaaata tatgtttcca ctagtagcat tttcaagccc tgctaaaatt ctaagtagcg
     2341 ttgttttccc acatccactt tcacctaata taacagttat ctcttcacta gatatattta
     2401 aagatatatt atcaagaact aaatgctctt tattatcaac taaatatttc ttatatatat
     2461 tctctagcaa aaatccactt ctaaccattt aaatcaacct gcttcccttt gctaaatttt
     2521 gttattatct ttgaaaatag ataatctgaa agtataccta aaaatccaat aacaaaaatt
     2581 cctaccaata caacatcagt tctagacata tcttttccat cagaaatcaa atatcctagt
     2641 cctgaagaag ctgcaataag ctctgctcca attatagccc taaaactata cccaagccct
     2701 aaactaagtc caacaacaat atccagcata gaattaggga ttattatttt ataaaaaatt
     2761 ctctttttag ataaattaaa agatttacct acttctatta atttattgtc acaatttttt
     2821 atacctttca atgtatttaa aaatattgga aaaaaggatg ccaaaataat aattataatt
     2881 ttagattctt cacctatacc aaaccataaa ataagcattg gcaccaaagc tagtggaggt
     2941 gtacttcgca taaattcaaa tagtggtttg aaatattcat acattttttt atttactcca
     3001 aataaaactc caagtggtat agccatcaat gaacttatta aaaaacctat taatatcctt
     3061 cttacactgg cataaacatt taagaaaata gaaccgtcat atatcatttt tataaaagta
     3121 tctaagacta tttcaggagg aggcagaata tagctactcc atatacccaa aacacatact
     3181 atcttccata aaattaaaat tattaaaaaa atataatatc ctttaagcct tttatgaatt
     3241 ttctcaaaca tataaaactc cttttaacat atctatccct gctacccttt ttatttttta
     3301 atatcttaaa taataattaa gctatatcat aagatttaat ctatgccaag tcttaatcca
     3361 cctgaactca ttctatcaga aggtgtagaa tctgttttta agccagacaa tctttcagat
     3421 ggttgtataa tgactatttg tccaggttca acaaattcca tatgataaga ttctcctgaa
     3481 gtctgaccaa taaaagtctt ccatgatagt tttgatactt taggatatgg ttctcttcca
     3541 gtccaagcta ctaatgcatc tgggtcaaca caacaaggtc cttcaagaat tagaggatta
     3601 ccatctgtta ttatagcaac atcttgattt tgacctgttt tatttactaa atgtgtagta
     3661 aaaagacctt tttgagatat tacccctgaa ccaatcaatt ttacactgta atttaattgt
     3721 tcagaaaatg ccaataagtt ttcactctca acataaatgg aatcacctgg gtcaattgat
     3781 attacatcta catggtaagc attttgagct aggtaaattt caccttcacc ttcaaccacc
     3841 attattggca tttgttctcc tgtcaatgac ctctgaacat gacctaataa tgctcttccc
     3901 aatccaccgc cattacttgg acctaacagc aatttttcaa aattaaaatt tcctttaaag
     3961 gctaccatag cacctttttt tgcaaaaaat ttaccacttc cccttgctat acacataagc
     4021 tcgtttgtaa catcaaattt aaacataata ttcacttata ttgcttaatt tagcaataat
     4081 ccctcctaat catatattta tatataatta tacagtaaaa ttacagcata gtctaaccta
     4141 cttcaaatat gaaatttgtt atttatacaa aaaataagaa cctgataata aagcaattta
     4201 actttttatc aggctcttat cctatttatc tcttagcgct atgacaactc ttactacttg
     4261 agcatccaga acaaccacat ccacaactgc taccattctt ttttgaattt ttaaccatat
     4321 acattacagc tagtgccatt aatactacta caatagctgc tataataaat gtagccatat
     4381 tcatcactcc ttatttatat gaattaattt ttcatataac ttaatttacc cattttttct
     4441 tttttcatat ttttatttga ataagttaaa actacaaaga ctactgctac tgctataaat
     4501 atagatatta ttgccccagc aatacttcct ccagtaccta aaactaaact tccaacttga
     4561 tttataagaa gagcaattat atacgctgtt aaagtttgga atcctaatgt aatccatgtc
     4621 catttccatg aacccatttc tctttttatg gctccaatag ctgcaaaaca aggtgcacaa
     4681 agcatattaa atgccataaa tgcaaacgca ctagctactg taaacatgct tgcaactgaa
     4741 gcaagaagtg ttgggtcttg ctctgttgca tctgatatac caaataaaac tccaaatgtt
     4801 ccaactacat tttccttagc tactagacct gtaactgttg ctactgatga ttgccaattt
     4861 ccaaatccaa gaggagcaaa tattggtgca actatatttc ctaagctagc taatatacta
     4921 tctccagcat ctaccatttg taatgaccaa ttaaatgatt gtaagaacca gattactcca
     4981 caagcaacaa atataattgt tcctgctttg attataaatg cttttcctct atcccacata
     5041 tgtattaaaa caccttttgc acttggaata tgatattgag gtaattccat aacaaatgga
     5101 gatggttctc ctgcaaataa actagttttc tttaatataa ttccacaaat gattatcatt
     5161 gcaataccta agaaatacat tgatggagct acccatgaag cacctccaaa taatgcccct
     5221 gcaaataatg ctattattgg aatcttagct ccacatggaa tgaatgtagt taacataata
     5281 gtcatttttc tatctctatc attctctata gtacgagttg acataactcc tggaactcca
     5341 caacctgaac ttataagcat tggtataaat gattttcctg aaagaccaaa cttacggaaa
     5401 attctatcca ttataaaagc tacacgtgac atgtacccac aatcttctaa tattgataat
     5461 aataagaata aaagcatgat ttgtggtaca aatcctagta ctgctccaac tccaccaatt
     5521 agaccatcaa ctacaagtcc ttgtaaccac tctgctacat ttaaagaagc tagaaaatta
     5581 cttacattgc cttgaataat ttctccaaac agtacatcat ttgtccaatc agttgctata
     5641 gtacccaatg aacttactgc tatatagtaa actccccaca ttataagtgc aaaaataggt
     5701 aatgctagta tacgatttgt tacaatctta tctatcttat ctgatacagt ttccttacct
     5761 tttcgattct tctttattat acttgaaatt attgaactaa taaattcata acgattggct
     5821 gttattatac tttcactatc atcatccaat tcatcttcac aatttctagt tatttcttca
     5881 atactattca atattgtttt agaaatatca agtttttgta taacattttc atctctctca
     5941 aatagcttta tagctaacca gcgtgtttct acatctatat atggagtctt ttcttctatt
     6001 attttttcaa ttttttctat tgcatcctta ctttcatcaa caaagggtaa tttgaaattt
     6061 aatttattgt tactacttgc aagttcaact gccttttcag cagcctccat aattccttga
     6121 cctttaactg ctgttacttc taccactggg caacctataa cttcagatag ttctttaata
     6181 tttatcttat caccattctt atttacaata tccatcatat taagagctat aacggtcggt
     6241 atacctagct ctaaaacttg agttgtaaga tataaatttc tctctatatt tgatgcatct
     6301 acaatattta ttactgcatc cggcttatca tctagcataa aattacgtgt aactacttct
     6361 tctaatgtat atggagataa agaataaata cctggtaaat ctactatttc cacatcttta
     6421 tttcctttta attttccacc ttttttttca actgttactc ctggccagtt accaacatat
     6481 tgtgatgagc ctgtaagccc attgaacatt gttgtcttac cacagttagg atttcctatg
     6541 agacctattt ttattgacat ttccattccc tccattatta tttaaataca tcactattca
     6601 tgatatagaa aatcattctc attatataat tacattaact ctactctaat taatctttat
     6661 tattccacaa taattttttc tgcatctgac ttacgaattg atagttcata atctctgact
     6721 gttacctcaa ttgggtcacc aagaggagct acttttctta taaatatatt aacgcctctt
     6781 gtaatcccca tatccattat acgtcttcgt gtagctcctt ctccttctaa ctttttgact
     6841 tttacagtct ctccacactt aatatccttc agtctgttca tgtcattacc tccctatatt
     6901 attattctac ttgccatttt tttatctaat gcaactctag tatcttttac gtctattatt
     6961 aagttaccat ctatcttaga aactatttta acatttgtac ctactacaaa acctatacta
     7021 tttaaaaaag tcctcgtctt atcacttcct gaaattttct ttataactac ttcttcacct
     7081 gtatttgata gtatcaatgg catcatataa tatccctcct cttaaactaa tttaaatcta
     7141 caagcaatcg ttatcttata tataaattac aataccttaa atcttaatct attaaaataa
     7201 tatcctattt tttccttctt tttacaattc ctaattttga tttaagtcta tcacaatagt
     7261 atattttttg caagtgatta ttgtattctt ttgatttttt ttctcatttg atttatttta
     7321 ttaataaaat cgtaaaaatt aatacaaaaa taaagatgct ctaaaatagt atactcaact
     7381 aatattaaag cacctttatt atatatatca ttacttacac attgctttat tcttttttaa
     7441 aagcaatttt ttcaaactct tttacaggca ttggcttaga aaacaaatat ccttgtacca
     7501 tgtcacaccc aatttgcttt agaaattcta cttgcgaaat agtctctaca ccttctgata
     7561 ttactttcat tttcaattcc tttgccattt taatgacatt agatataaca attttttcat
     7621 tattactctc atctttttct gtaaaaaatg ctctatctag ttttaatatg tcaacaggca
     7681 attcttttaa aagattaaga gaggaatatc ccgtaccaaa atcatccata gagattacaa
     7741 atcccttttc tttcaaattg ttcattgtat caagtaaccc ttctatatta tctaatactg
     7801 ctgtttctgt tagctctaat tctataagtc ttgtcggaat tttgtattta tctacaatta
     7861 atttaaactt ctctataaaa ttactattta caaaatgcat tcgagacaag tttacagata
     7921 taggtactac tttttgtcct tcatccatcc attctctaat ctttttacac acttcctcaa
     7981 atacatagaa atcaatattt acaataaaac cattcctctc aaatacagga ataaattcta
     8041 ttggtggtat tagacctttt tgtggattat cccatcgaac aagagcctct gcccctataa
     8101 tttgataatc tgaaagacta tatttaggtt gaaaataaac aataaattct ccattattaa
     8161 gagcatctac cattgaattt tcaatttctt tctcttttaa tattttttta tgcatttcct
     8221 tatcgtaaaa agcaaatgaa tttttatgac caccttttat agtttttctt gcagtattag
     8281 ctctatccat aatagtattt atgtcattat cacttttact tattttatat ataccacaat
     8341 ctaaaatcaa tttataatag tttattttag gattactaag tatttgaacc ttattgtaaa
     8401 tattgtttaa ccttgtttta atatcatcat ctgtcttata cttaataaga agaacaaaat
     8461 ggtcagcaga aactcttgca aaaatttcat cttcctttaa ttcacttgca atagtatttg
     8521 atatatgtat caaagtatca tttcccatat catatccaaa catatcattt atatatttaa
     8581 atttgtcaac atctatataa aacaatgcat attcttctgg attattttta gcaaacagtt
     8641 tattcgaatt tattttaaat ttatctatac tatctgctcc tgtcaatggg tctgtatatg
     8701 ctatcttctt tagataatcc tctttcttct ttttattata aattatatat ttaatagcaa
     8761 ttaagaataa aatacataca aaagaaataa tttgtattga atattcttta aaaatcacac
     8821 caaatggtac tttataaggt ttgtcaatag tattagaata tatacaatca cttaattctt
     8881 cttcagtaat tttgtctatt gatttattta aaatagatac taatataggg tcagttttat
     8941 ttgatacccc tatacgcatc ccatagcttg tatctaaaac agatataaca gataaattct
     9001 cactctttcc ttctcttaaa agttcatcaa actgataaga actaagtatc attaagtttg
     9061 ctttaccttc atttatagcg tttagacaac tttctatact cttataagat gttgtcttag
     9121 cattttcaaa acttttttct atataagaat cgatagactt ataactactc gttaaagcaa
     9181 tatttaaatc attattgaga ttggtatcca gttttcctac cataatcatt ggaatattta
     9241 tgtatggatt tgtcaatttc atattaaatc gttttgcctt atcattttca ttgattgagc
     9301 cacatagcat atttgcctta ccacttttta tgtaatcaag actctcatta taattttttg
     9361 ttttaatata ttcaaatttt ataccacatt tttttgatat aattgacatt aaattggaac
     9421 ttataccttt aaacgaattg ctttccttat cataatactc tacaggagac cactctgaat
     9481 catataccat agtaagtttt gggtttgctt taataaaatt catttcttgc cgagtaaatg
     9541 ctatggaatt attgacctct cttttgaaat attttttata taattccaac tcataataca
     9601 tatcatttat ttttaattct tttaaagcat aatctaattc ttttaccaaa ttaggtttat
     9661 attttgcagt agcaaaataa aaaggctcta ctgaaaattt gctaactatt ttcattcctt
     9721 tagcatttat aatacttcca cagacaaatg catctgcctt tttttcattg acagactcaa
     9781 ttaattcatc aatagaatta cagtagatat attcaactga aaaattattt tcttctctat
     9841 acttctcaaa tgcaatatta tgaaagtttc ctctaagtac agctactctc attcccttaa
     9901 aggaacttat atcttcataa aacatattat tgttactatc ttttgtatat agtacactat
     9961 attcatatcc tgaatttagt ttaggaaaat taaatagttt atttctttca tcattcttct
    10021 gtaaaggtcc aagtaagtct atttcacctt tttctagtct gtctaaacat tcttcccaag
    10081 acccttttac aaattcatat tcccatcttg tatattttga gatttctctt agataatcat
    10141 aaccatatcc tgtataagaa tgctcttttg atgaggatat tattccgtag tcatcacaat
    10201 atccaactct tatttttttt gaattttcag actcatcagc aaagcatagt atattgttaa
    10261 aacatgatat aataagtaaa aatatcaaag aaagtatcaa gaagtgcttt ttaaatgctt
    10321 tcatatttaa aaacctccaa tttgccaact tactttaact tatttggttt tctagttgta
    10381 actactttta ttgtaatggt aaatatatca aattgcaata ttctcatata atattcttat
    10441 tcaactataa tgaatttatt attatctatt taaatacact atctaatatt agtttcttat
    10501 aagtcataaa agcacaacta acactataat tctagtgatt aattttattt caaaaaatat
    10561 aataacacta ctaatatttt aaatatcaat agtgtcataa aaacttttca gattaattaa
    10621 atctttctat atattttata ataatctaaa tcatgctaaa tatttagaca gaaaactttt
    10681 ttctttaata gtaaatactt ttgtttgaat atcagaataa ttatttgaat atctatctat
    10741 aaaaccatga tttgcatcta aaatctcaat ttggatattt tgtttcctag aaagttggta
    10801 agaaatagaa tcaacatcaa atgaatcgtg ctttgcaaat actaataagg taggacattt
    10861 tggaattaca tccatgtaat ctcgaatccg agagccataa caacaaatta tgccatcaca
    10921 aaaagaatct tcactgcatc tccatgcaat agttgctccc acactatacc cagcaacaaa
    10981 tacatattta tactctgatt ttaaatcatt tataagcttt tcaacttttt tataaatatc
    11041 aaaaccaaca ttatttataa aaaaatcata tgcctcttgg acttttgaat atggaaaaac
    11101 tcttttttct cccagaagtt caggacaaaa tacatcatat ccatatttat aatacttttg
    11161 acaaacatct tctataaagc tattaatccc atagatttca tgtaaaacaa caatagctac
    11221 ataactttta tgtatatact ttatcagatt atccaccacc tttacaaaac agcaaatttt
    11281 ttatacttag tttaatatat tcttctttac atgtcaattt acttttctaa aatcaaattt
    11341 atttttggat taatcattta tttttaaaag ttgtgcatta ttactattaa attattctct
    11401 attattaaaa atataatcgt tagaaataaa aaataaagta caaataatta aaaaatatct
    11461 cactcataac tttaaaaatt gtaagtgaga tactcctaaa atctacttta tattaaattt
    11521 attttccaaa atatctattt aataatccta agaatgcttt accatgtcta gcttcatctt
    11581 tacacatttc atgtactgta tcatgtatag catctaatcc taattctttt gctttcttag
    11641 ctaattttaa ttttccatca gttgcaccat actcagcatc aactctaact cttaagtttt
    11701 cttttgtatc tgcaactaca acttctccaa gaagttctgc aaatttagca gcatgttcag
    11761 cttcttcaag agctattctc ttataagctt ctcctacttc tggataacct tctctatcag
    11821 cttgtctact cattgctaaa tacattccta cttctgtaca ctcaccaaca aagttagctc
    11881 ttaatccttc gattatttct tcatctacac cttgagctac tcctattctg tgctcatcag
    11941 cccaattttt ttcgcctttc atttcttcaa atttatcagc tccaacttta catactggac
    12001 attgtgctgg tgcagcatct ccttcatgta tatatccaca tactgtacaa acaaatttct
    12061 tcataattac tttcctccta aattttatat atcaaattaa attttttatt tattaagtta
    12121 gtctatatat acccgcacca tataccttta aacaattttt ttaaaagttt tttctaaata
    12181 cttattttcc ctgtgtcaat aatatgtttc tataataatg tccctttaat tttatcaatt
    12241 ctttatgatt tcctgattcc acaattctac cattttctaa aactattata ttatcacaat
    12301 ttgatatggt acttaatcta tgagctataa ttatacttgt ctttccataa gttaaattat
    12361 ttattgcttc ttgaatttta gcttctgttg ctatatctat gttacttgta gcctcatcca
    12421 aaataagtat tggagcattt tttaatactg ctctagcaat acttattaat tgtttttgac
    12481 cttcacttaa atcttcaccc ctatccgata atacagtact atatccttca cgcattttaa
    12541 ctataaattt atgtgcattt gctattttac tagcatatat aacttcttca tcagtagcct
    12601 ccagatttcc ataccttata ttttccataa ctgtatcatt gaatatataa ccttcttgtg
    12661 gaactactcc aaaacaagtt ctcaaatagt ttctgttaat agagtatata tccttaccat
    12721 ctaaaaatat acttccttca tctatatgat aaaaatttgt aattagattt ataatagttg
    12781 tttttccact tcccgtctct cctatgatgg catttgatgt gccagatttg atttttaggt
    12841 ttatattatc taaaattttc ttttttgaat cataagaaaa acttacatct ttaagctcaa
    12901 tttctccctt tacaaaatga gagtttaaat taccatcttt attatcgtta atctcacttt
    12961 taaacatgtt atgcacttct tgctcttctt gttcatctat aatttcaaaa actctttcac
    13021 atccagctat ggctgaaagt aatgtattaa atagatttgc taattcattt aaaggtcttg
    13081 tgaattgtct tacataactt aaaaaactag ctataacccc tatggttata atatctttag
    13141 atgcaagaat gcctcctata atacatatag ttgcaaatcc tatattacta atcacattca
    13201 taataggcat taaaagagaa gtgtaaattt gcgatttaag ggaaatattt agaagtctat
    13261 cattgacttc tttaaaatca tttacagatt ttctttcata attaaaagat tttactacat
    13321 ttatgtttga tatcatttct tcaatatgtg catttagttt tcccaactca acttgttgtt
    13381 gcttaaaaaa aactctagtt ctctttgcaa taaaattact cacaaacatt actatcggta
    13441 ttaatattac actgcaaaag gttaaaattg ggcttaatac aaacatcata ataaaagtca
    13501 tcaatatgtt taagattcct gtaattaact gtactaatga atttgatatt cctgtactga
    13561 tattatcgac atcatttgtc actctactca taatatctcc agtagaactt ttatcaaaaa
    13621 aggttattgg tagattttta aattttaaaa ataaattact tctcatttct ctgactatac
    13681 tttgagataa tctagctgta catattccct tagacagttt aactgaaaac tcaattatat
    13741 aaaatgaaag taaaataatt attcctttta agaaaatgga ttttgaataa gtatcgatat
    13801 ctattatgtc aactaccttt ccaatagtat atggtattac agttgatata attgaatcaa
    13861 ttattactag caaaaaaata aatctagtaa tcttcttatc ttttttaaag tacataaaaa
    13921 ttcttttaaa cgttttttta aaatctttag gcttttcttt tttctttaat ctactgacct
    13981 tctgatttcc agtacttaaa ttcaatatac ttcctcctta atttacatct tcacttacta
    14041 aattttgcaa cgcaaaaatt tttttataaa ttggagaact aaccagtaaa tcttcatgtg
    14101 tagcaaatcc aaccacctca ccctcatcca agactaaaat cttatcacag tcaattatag
    14161 aactaatctt ttgtgaaata gttattacag tacatttctt actttccttt tctcttaaat
    14221 tacttatata ctcttttata ttctttctta taattgtctc tgtattaata tccattgcac
    14281 taaaacaatc atcaagaatt aatatttgtg gttttttggc tatagctcta gctatagtaa
    14341 ttctttgtct ctgtcctcct gataaatttt tacccctttg gaataaaact tcatcataag
    14401 tattttcgag ttttgttata aattgatttg catttgctat ttcacatgat tttacaacat
    14461 cctctttgca tatatctcta tttcccataa ctacattctc caatatactt tttccaaaca
    14521 cactactatt ttgaaatact acacctattc ttgcccttaa actttcttca gaaatactgt
    14581 atatactacg tcctccaatc ttaatatcac ctttatcaat tatatataat ttttgaatta
    14641 aatttactaa agaagtcttt cctgaaccag tttgaccaat cacaccaata ttttgacctt
    14701 cttctatttt aaaggatacg ttttttaata ttttttcatc tgtatttttg ttatacgaaa
    14761 aatctacatt ttcaaaaatt atatcacagt ttacaaactt taaatctttt tctccaacat
    14821 ctaaactttc ttctctctca attacttctt cattctcatc aaccaaaacc tcacttattc
    14881 tctcataaga tgcttttgcc ctaacaaaaa cattaaaaac atgtgaaatc attataagag
    14941 atgttaatat tctagtcata tagtttacat aagcaactat aatcccaatt ttaatttctc
    15001 ctgaataaat taaatgcctt ccaatcagta atattattat tatggaaaca ttaatgatta
    15061 ttgtaattgt cggtctaaaa aatgacatca tagttgtagc ttttctacta tttttaaaaa
    15121 gttcatcatt taactcttca aagctttggt tctcataatc gtatctacaa aatagtttaa
    15181 ctacttttat tccacttaaa aactgtctta attttgaatt tactttatct gtactttctt
    15241 gtacattttt aaataatcta taccctatct tcatattaag caatattata ataaatataa
    15301 ttaaaactgc aattagaata attattgaca tctttaaatt taattttata gctataatta
    15361 aacttcctat acacattatt ggtgacttta taaaaactct catcattcca ttaacgaatt
    15421 gttgcatttg attgatatca ttagtaattc ttgttattaa tgaagctcta ccaaatttat
    15481 ctatttgttt taatgagtat gtatttatct tttcaaatac atcctgtctt aaatcataag
    15541 aaaaatgctg agaaactctt gccgagatta cacttcttat tattgcaaat attgcaccta
    15601 aaaaagttat acctatcatt atagctcctg ttgatagtat gtagtctaag ttcttatttg
    15661 aaacccctat atctattatt tttgccatta tagttggttg agctaaatca caaaaagctt
    15721 ccaatgttaa aaaaaatact gctgttagaa ataatactat atattttttt atgtattttt
    15781 taaaatacct cattcaatca tcccttcata gaattttctc attaccaaaa gtatatcata
    15841 atggctaatt taattgatta tatcaattat aaatatttga tttattatta atttttatac
    15901 catatatttt ataaattatt tgtttttatt tcctatattt atat
//
LOCUS       DAMKMI010000094        15702 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMKMI010000094 DAMKMI010000000
VERSION     DAMKMI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.097"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            189..2423
                     /locus_tag="PC429_002597"
     CDS             189..2423
                     /locus_tag="PC429_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3848096.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2454..3287
                     /locus_tag="PC429_002598"
     CDS             2454..3287
                     /locus_tag="PC429_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3848097.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3527..5260
                     /locus_tag="PC429_002599"
     CDS             3527..5260
                     /locus_tag="PC429_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3848098.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5652..6210
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5721..5749
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6740..6844)
                     /locus_tag="PC429_002600"
     CDS             complement(6740..6844)
                     /locus_tag="PC429_002600"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF3848099.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7469..7714
                     /locus_tag="PC429_002601"
     CDS             7469..7714
                     /locus_tag="PC429_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848100.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            8709..9358
                     /locus_tag="PC429_002602"
                     /pseudo
     CDS             8709..9358
                     /locus_tag="PC429_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            9899..11254
                     /locus_tag="PC429_002603"
     CDS             9899..11254
                     /locus_tag="PC429_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3848101.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11372..12040)
                     /locus_tag="PC429_002604"
     CDS             complement(11372..12040)
                     /locus_tag="PC429_002604"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF3848102.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12107..12535)
                     /locus_tag="PC429_002605"
     CDS             complement(12107..12535)
                     /locus_tag="PC429_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF3848103.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12642..13235
                     /locus_tag="PC429_002606"
     CDS             12642..13235
                     /locus_tag="PC429_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF3848104.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13354..14289
                     /locus_tag="PC429_002607"
     CDS             13354..14289
                     /locus_tag="PC429_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF3848105.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(14560..15504)
                     /locus_tag="PC429_002608"
     CDS             complement(14560..15504)
                     /locus_tag="PC429_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF3848106.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         5959 a         2064 c         1977 g         5702 t
ORIGIN      
        1 ttatctaata ttattactaa actaaaaaat atttgtattt gtagcattgg agcaagtaac
       61 ttaatgtata ttcattttaa atacttattt aaaatttagc atttaaatta tttacaaatt
      121 aacatttaaa tacttctagc ataaatacag ttgtcattaa aatttaaagt atcttatgga
      181 ggaataccat gcaaaatcat aattatataa aaatgttaaa taaaaaggta gaagtattat
      241 ctaaggaatt agatagtaga aattttaaat taaaaacctt aaatgaaata gatttttttt
      301 ctaaaatacc aaacagagaa tttatgtacc aacatattga tgactgtatt tcgaaaaata
      361 tacatatagg aatcatgtta ttaaatatcg ataactttaa attatttaat gatatatatg
      421 gatacctagt cggagaaaat ttattaatta ctctaactca aaacctattg cactttattc
      481 cagaatcaaa gggaatagtc gcttatttaa aaggagaagg ttttttaatt gtacttccaa
      541 atgtatcaaa taaagagtta aataatatgg gtgcaactat tatagagagc gcccgagaat
      601 taaaaataaa acttcctaat gaaaaagaaa tatactccaa tgttacattt agtatcggca
      661 gcactatctg gaatggtgaa ccaaacatga ctactttgat gcttttcaac caagtatata
      721 gtgctttaaa aaaggctaaa agtgaaggta aaaatagata cattctattt gattttaaag
      781 ataatagttt ttacaataaa ttagaaaatg ataaggagtt cttatcagaa atatcacatg
      841 ttctacaaac taaacaaatt gaagtatttt accaaccact ttataatata aaaaataata
      901 ctatagttgg atgtgaggca cttgcaagat ggagacaccc tattaaagga cttcttactc
      961 cagataaatt tattccttta tttgaacagt ttggtctgat aacggaatta gatttgcatt
     1021 tatttgaaga gaactgtaaa aatataagaa gatggattga taacaatgat gtttttgttc
     1081 caatttgttg taacttttct cgtcttcatt tttttaaccc taattttcca agtatgctaa
     1141 aaggtattac tgaaaagtac agcgtctcta catcaaattt tagcattgaa ataacagaaa
     1201 atatattaat agaaaataca aatacaatta ttcaacaact agctgaactt cgttcttatg
     1261 gtttttctgt atcaatggac gattttggtt ctggatattc atcttttggt atgttgcaaa
     1321 atctaccttt tgatataatc aaaattgata ggatattttt atctcataat ttaaatgatt
     1381 ttaaaaatac aaccattttg tactctataa taaacattgc aagagctctt gggatgctta
     1441 cagtatgtga aggtgttgaa acagaacaac atgtagaatt tattaaaagt ataaattgtg
     1501 atatcgccca aggttattat tatgcaaaac ccatggagtg caaagaattt gaaaaattac
     1561 tatctaaaga gaataattca gagaaaatgt ttataaaata ttctgatata ggaaaatctt
     1621 taatagagaa gatatttgat ggatttttta ttatgcaaga tatgaaaaaa ctaagagcta
     1681 atgtcactga agacattcaa tggtttgatt tttacaacaa aactgaaatt acatcttttc
     1741 ctaaagtaat agagcatttt gaaaaacata ttaaaggtaa aaaattttct attctttaca
     1801 aaagtataac cccacataat actgaatcag attcattatt aattagtgga gaagccgttt
     1861 tatcagatga agcttctgaa catcaaaaat atagcaattt ctactttagt gcaaattgta
     1921 taatttcaga aaataaatta ctcttagcaa aattacattt ggatttaatt aaaactgctg
     1981 gctatataaa tgaatttgaa tcatattcaa tacctaaaat gacaaataca catcttgatg
     2041 aaagtaatat tttatcacaa ttttattcaa tgttgcctgt aggtataatt cgctacgatt
     2101 tatttggtga tatgattatt acttatatga atgaagaaat gtttgatatt ataggttata
     2161 caaaagaaca gttttttggt gagatgggcg gaaatttaag agcaattgtt catccagatg
     2221 atttagactt tgtttataaa aaaagtttgg atatgattga cacaaataaa attgaaccat
     2281 ttttatatag attcataaaa cgagatggtc aaattgtcac agttatgtat acacaatgta
     2341 acctttctgc aattgatgga aggtcgattg cccagggtat gtatgtagat gtaaataaaa
     2401 taaaaaattt aacaaaaatc tagtacaaag tattttattt tggagaaatt aatatgttta
     2461 aagaaatttt tttaagaact tttccaggat ttctaattat aagagattct aactacagaa
     2521 ttatatttat aaatgataat ttaaaaaatt taattaaaag ttatatgcaa gataatccac
     2581 ttggaatgac taatatagaa atagctaaga aactccctga taatattgct aaatttttta
     2641 cggattctca caatattcaa ttagattggg aaaaaaatta tccttatgat aaaatttcaa
     2701 attggatttt agagtttaaa aaagatacta caagctattg gaatgtttta gaatataaag
     2761 tagatgttga tgaaaaaacc tacataatta ctatggccaa tgatataaca aaactctatg
     2821 aagaaaataa gagaaatctt cactactcaa ttactgaccc attaactgga gcatataata
     2881 gaaaatattt aaatgataga tttgacatat ttataggaga ttatattgta ctaattgact
     2941 tagataattt caaaatgata aatgactatg aaggtcataa tgtaggagac aagattttat
     3001 gtgattttgt ttcactttta aataaagaat taataaattc tacttctatt ataagattag
     3061 gaggagatga atttattgtt atattctcta gtgatgttga caaaaattat gtttattcac
     3121 aactagaagc cttaagagaa aattttttaa aagtattctc taagtataag tatctttcat
     3181 ttagttatgg tgttgataca gtaaaaagaa atttaaaatt gacaatagtt gaattggata
     3241 aaaaaatgta taaaaacaag aaaaaatttg ataaaaatga ttattaatat tgttatttta
     3301 aaataaatta aaattatctt tcttttttta aacttgtaca ttagtttata aatatgtatc
     3361 tatacaagat attgacatta agtactgaag catatattgt ttcagttctt ttttgttaaa
     3421 attaacttat ttaacaaaga tatcatagtt taacaaagct tttaatttaa atatattgat
     3481 gtataatata attaaaatat aatagcaaaa agaaaggagg aatatcatga gcatactcct
     3541 aaaaaaagca cccaaattag caaaacatat tataacaagt ttttatataa atcgggatat
     3601 tgatgaagta ttaaaatacc tttgtgaaaa tgttacatgg atcggaccag gagaacagga
     3661 atttctcact tcttttaatg aaataaaaaa ttatttttat gcaggtcaag atgaaattcc
     3721 ttcttgtgat ataaataatg atatatttga aactgtaagt gaagatgaga atagatgtat
     3781 ggtattagga agatatactg ttcgaactaa agaaaatact caaatgattt tagaggtaaa
     3841 tcaacgttgt acatttgaga taattgagga tagaggaaaa ttattagtaa agcacatgca
     3901 catatcaaac ccttatggtg aaatgcaact tgatgaatac tttcccacta aaattggaac
     3961 tcaaagttat gattatttgc aaagattact aaaggaaaaa acagaagtta ttgaaatgat
     4021 tacaaataat atcaatggtg gcttaaaagg tagcaatgat gataatacat actcattttt
     4081 ctatgtcaat gaaggattgc ccaaaatatt aggatacaca tatgatgagt tcatggaaat
     4141 gagtggtggt tcagcagtgg gtgctgtata tccaccagac ttacctaaag cactagaaga
     4201 ttgtgaacga tgttttgcaa aaggacctac atattcatct gaatatagaa tccgaaaaaa
     4261 agatggaaca ttaatgtggg tattagattc tggaatgaaa tcattaaata gtgatggcat
     4321 agtaaaaatt aatagtatta ttactgacat aacccaacta aaaaacatag aatctgaact
     4381 aaaattagaa agagagcgat atcgtattgc tttgcaaaat attacagata tcatgtttga
     4441 gtatgatatt gaaaacgata cccttataaa atatcaacgt gtagaaattg acaaaaaaat
     4501 tgaattagaa aattttgaaa caaaaaatta ctccaaacta ttagaatctg gtaaaattat
     4561 tcaccttgat gatattggaa aactactaga agtacttcat ggaaatcttc gtgaaacaat
     4621 tgagataaga caagtaaatt cattaactaa aaatgaatgg agatggattc gtgttcaatg
     4681 ttcagttata tatgatagtg accataaccc tatcaaaaca ataggcgttt taaaagacat
     4741 aactgaagat aaatctaaat tagaaagctt aattaatcag gctcaacgtg acccactaac
     4801 acaattatat aatcaaagag tatcacaaaa tcttattgaa gagtatctat gtagttctga
     4861 ttccaaaaat aatgatgctc tattagttat tgatattgat gattttaaaa ctgtaaatga
     4921 tacttttggg catttagagg gaaatgaagt tttagttgct gtatcaaaaa tactattaca
     4981 taacacacat gataaagaca tagttgctcg aattggtgga gatgaattta caatatttat
     5041 aaaatcatta acaaaggatt taataataaa aattactaat gatattttaa atgatgcaag
     5101 taaaataaaa gtaaaggaca atcataaaat aacattaagt attggtattg ccttcactga
     5161 tgattcaact aaattatata aagatttatt ttctaaagct gataaagctc tttatctatc
     5221 aaaagcagat ggcaaaaatt gttatagtac atacgaataa taaagctatt tatacgacca
     5281 agaatgaacc tttaaattta atttcatagt ctaacaatca aagagttgaa gctaatctta
     5341 tagctagtca actagttcct agaaaaaatt taaacaatat atttattgat actgctcatt
     5401 tttctaaaga gcaaatttat ataaaaaatt aaacttttat gtacatacag atactaatga
     5461 taaacagtta tcattagtat cttctatctt aaaacaaaat gataatttat ctatataaaa
     5521 aagtgctttg ttgacagagt tcactttttt atataggtac agaatttaag tataaataca
     5581 tgttttattc tatacatttt agttaatgta aaatttcttg attgtactct ctaacttttc
     5641 aacattatac tatttatata ccacttagtt aatataaacc ccttagctgg ttctagtgct
     5701 ttttctatga ttttagtcat atttacatac cacttagtta atataaaacc aatttagatg
     5761 atgtatatgt gcaaaaggaa gaaataattt acataccact tagttaatat aaaacgctac
     5821 taaacttgat atacatgacc attataataa taaatttaca taccacttag ttaatataaa
     5881 acttccataa aaacatcccc tttttattct tcttccttat atttacatac cacttagtta
     5941 atataaaaca gtcttttttt aatgcagaga tgcaagaagt tttataattt acataccact
     6001 tacttaatat aaaactatca gtatttaagt aataatttaa gtaatatagg atttacatac
     6061 cacttagtta atataaaacg gtcactacaa cttatacttc cagtctaacg atttgaattt
     6121 acataccact tagttaatat aaaacatatt ttggatttat taatataaac ataattaaaa
     6181 aatttacata ccacttagtt aatataaaac cccaaaataa actgggcatt ttcaatactt
     6241 acacatatgc aactctctta aatttgcagt gaaccatgag tagtgcaaat gataaagctt
     6301 atcatatacc ctcaacacct tatatttcaa gcgttaagct ctactttgta acaaatatcg
     6361 ctcactgcaa aactatttac atttttatta tatcataaat atattatttt tgaatatcta
     6421 taccaatttg tggtataata aaagcaagaa gaactacaat ctatttagac gtagagtgaa
     6481 gttcaaatac taatgtttat tctttttgaa cttgattttt agttcaaaac taaagtcact
     6541 cctgcctgtg tgaactatac ccgctgtttt atacagttaa aacatgtaaa aatagttata
     6601 aagtactagt gtaactcatt agtactcttt ttattatatt catataatta atatctatat
     6661 tttaaagttt actatctaaa aaagagcacg tcaaataaaa ccctagatat ttaatttttt
     6721 tagagatact taaaataaat tatgctctct ctgtacttaa atatttccta aattcaaatg
     6781 gagacataga atttaatcta ttttgatatc taaaattatt atagtagtat atataatcat
     6841 ctattgcttt aactaattat tcatatgtat caaatttcct taaatagtac atttctacct
     6901 ttaaaattcc ccaaaatcct tccataggag cattgtcaag gcaacgacca actcttgtat
     6961 aatatatgta tatctaacac cattaagaaa tatatatttt ataatatata tttcttcatt
     7021 taatgcactt agaaaagcaa agaaaataca gattagtaca ctttttagag aaaaataaaa
     7081 ttaatacatc taaaatatta aaaaatactt tagataatct aaagacacat ctcaaagttt
     7141 ttaactaaat tataaaacta attacaaatg ctatgtaacg ataataaata aaaaatttac
     7201 ttcgcttaag cgacgtgtcg ttgaaatatt tcatgttgcc aactggttcg taagttgata
     7261 agaaaagaac actaattatt tagagttctt ttcttagcta caagtggatt ataatacttt
     7321 taaatctgtg agaaaatcat atataataat gatatgaaca agtaaagaaa atacaaaaat
     7381 gacaaaatta taaataaaat attataaaat cataaggtca taattgttat attgtagaaa
     7441 caaagtaaag agagagggtg gagaataaat gaatcaaaaa aaaataaaga ttagtattaa
     7501 aatggtaatt ataggtatat ttgctgtaat tattgctttt gtattatcca gattattcgt
     7561 tgagtatatt gatgaattat taaggtctaa ttggcaatat cagctatttg aaagtgagag
     7621 ttcatatata gtatttgcca attgtattat gttctcgaat atattattag aaatattttt
     7681 aatatatatt tgtagaaaat ttagaaaaat ctaatatggt caacgtattt cgaatcgaat
     7741 acttcatgat tatttagtct attgtgctag ttaaagttta attcccatat aaagtaaatt
     7801 gattttagta aaacaaatca cccctaaggg ttagtttatg gataagtcat ccaaatatta
     7861 actctttaat ttttgctata gattcatctt tgctcataac tttacttata aaaaatgaaa
     7921 tattataagc tagaactttt attctaattc tagtcataaa tcctagcata gagttacttt
     7981 ttttatatta taaataaaac tatacaacaa agataatatt tagtaaaaat aactttcatt
     8041 ttaactaaac ttaaaattca attactctct aaattgacgt ttaggaatta gttcaaaata
     8101 aaaaagagtg tatatattga aattactaca tttcaatata tacactcttt ttaaatttct
     8161 cagttttcca tggaacttta ctttatttat ttctgtatta gttgtatttt ttatgcattt
     8221 ttctttaaaa tcatattttc tgattcaact cttatattat aaaatttctt tactaaatta
     8281 ttctcttttt ctatatcttc atatatattt tttaatcgcc ttttacctaa tcttttatct
     8341 aacattacta atgatttaac taatatatta tatgatttta tcgcatcttc tattgagata
     8401 tttaaacata tatttactgc ttcataaaaa tcaaattcat taaaaaatcc atttttattt
     8461 acaatcttat atccctttgc atctctttct aaatactctt cctaaatact cttcaaaact
     8521 ttcatcatct aatctaaatc ctatatcact atgaaaaata ggttttgcat ttggatatag
     8581 ttctattgtt ttatcaaaag tttcgaatac caagttatta ttattcgatt ttgcaacagt
     8641 ccactataaa caataataag tttgttgctc caaataatgt aacacttgta gaaagatgtt
     8701 acagatatat aaaagataga gaatgtagtt tttctcctaa tactattaga aatagaaaaa
     8761 atatgattaa aaatcaaatt gaagttttct ttaaagatat gaaattaata gatataactc
     8821 cttctatttt acagtcttat ataaataaca tatacaatga gcacatgtta aattcaacaa
     8881 aaaaccaagt cgattttata aaatcagtat tgaaagaatc ttatagatta aaagaaatat
     8941 cagaaaatat ttgtgatttt gttacaaccc caatcaaaaa aaatagttca acttctaagc
     9001 tttatacaaa acaaaaagct caattattat tagaaaaatc aaaaaatatt cctataggta
     9061 taccaatttt tttgatgcta actcttggat tacgttttgg tgaagctgta tcacttatat
     9121 ggtcagatgt agaccttgat aaaaaatctc acatgtaaat caaacattag tttatgtata
     9181 caataaaata atcttcaaag gccctaaaac ccctagattc aaaagaagat tatttgttcc
     9241 tgatgaactt ataagtttgt ttaaagggga aaaattaaaa caaaataagt taaaattaca
     9301 gggtatattg aaaaatgaat ttgatttgat atgcttaaat aaaagattca acccgtgagt
     9361 tcaaccaact tttgttgttc attttaaaaa actattaaga gataataatc ctagagatat
     9421 aaaagtacat gaattaatac atgcaaatgc gactttgata ttactttcag gtactaacat
     9481 aaaagctatt tataaaatat tagtacatgc agatataaaa atagctatga atatatattc
     9541 catattctca cttattagat gaaatgaata aagaaacatc tgaaaatata agcaaaatac
     9601 tattcaaata atgttcagtt ttatgtcaat taaatgtcac tcaaagtgct ttttttccat
     9661 ttgtcagtta aatgtcactt aaagtacctt tttcctattt gtcagttaaa tgtcagtaat
     9721 atataaataa gatatattta taagaccctt aaaatcacta ttatttaata aaatttataa
     9781 ttatatacta tttgctgttg acattggtat tataccatag tttatcattt atattagaat
     9841 aaaattatta attaatagtt tcgtaatttt ataatagatt tacagggagt gataataaat
     9901 ggatacaaaa atatctataa acgaaaacat gagtctaggt aaaagatttt ttaaatacct
     9961 agcaccatct gtagttgcaa tgtgggtttt ctcactatat acaatggttg atggaatatt
    10021 tgtaagtaaa ggagttggag aattagcttt agctgctgtc aatatatcaa tgccttttat
    10081 taattttata tttgcagttt ctctattatt ttcaactggt gcatctacca taattgcaat
    10141 ctatctgggg aaaaaagata ttaaatctgc taacgaagtc ttttcattta acttagtatc
    10201 aataattata ttatcaataa ttatattagc tataactttt tttaatttag atagattagc
    10261 actattttta ggagctacag aatctacaat tggtatggtt aaagattatt taggaataat
    10321 aatatttttt aacggattct ttatagtgtc ttattcttta gaagttataa taaaaactga
    10381 tggctttcca atattagcta ctgttggtgt aataatatct gctcttacaa acatcatact
    10441 tgattacttg tttgtaattg aatttggatg gggtgtaaaa ggtgctggta ttgcaactgg
    10501 tttgtctcaa gtcttttcta ctattttctt cttaatacat tttttaagaa aaaactctac
    10561 tttgaatttt tcaaaattta gaattgattt taagacactt agaaaaattg tatttatagg
    10621 ttttcctgat tcaactactg aattaagttg tggtatagtt gttttacttt ttaacttaag
    10681 tcttactaaa tatattggag aaaatgctct tatttattat agtgtcataa attatataaa
    10741 tacattagta cttatgacta tgatgggtat aactcaaggt atgcaaccac ttactagctt
    10801 ttattatggt gcaggaaata tagataatgt aaaaaaatta ttaaaaatgg ggataaaagc
    10861 cacaataata gcatcagttg ctgtatttgc aatttgtatg gctttttcag gaccaatagt
    10921 ctctttattt atacatccag aagaaacaat gctattcaat gaaggagttc gtgtatttaa
    10981 aatattctct atatctttct tattggtagg aattaatgta ataatttctg gattctttgt
    11041 atcagtagaa aaaccatcaa tttctactgt tatatctctt ggtagagggc ttgtaatagt
    11101 tgttttaagt ctaatttcaa tgatactaat atttggtgga caaggaatat ggatgactac
    11161 tattgtttct gagtttatat gtcttatttt atcattagtt tttctaaaaa agaatttttc
    11221 tacattagac tctaatctaa ataaggttgc ttagatgata agagtgacaa tatatgaaaa
    11281 tatctaaata aaaaatacct attatacaat cttgcaaata ttaaaccttt gcaaaaatta
    11341 ggattatata ataggtattt tatttatgta attattttat ttctaaatat ccatagccat
    11401 cttttattaa agttcctttt acttcatatt ctacaggttt tttaaatatt ggaccatcta
    11461 cgacaaatgt atgatactct ccatgttctc cacatgggtc tgcaccagca ttttttatct
    11521 tctccacaag ttctctagtt aatttttttc ctaaaaattc ctctcctaag tgctttaagt
    11581 ttacttttgt tacaacacta caaaatccac tgtctataaa ttcatatact aaatcctctc
    11641 tactctcttg ccataatggt aaatctactt tcataccaac tgcttcacat ctatcagtat
    11701 cccattttct atgactttct atatctatat ctccatatgc acatatcgtt gctcccatgt
    11761 ttttagcttt tattaaagcc ctctcaaagc tctcttcata tccttctacg ccacactcag
    11821 caactaataa aggtaatcct atgttgctac ttacttgctt aagcatatta tattctaaat
    11881 tatgtgtcca tgactttcca tgttcttcac ttatagttgt caagagtgcc acaggcttat
    11941 atcctttttt caacattcga tttaatgcta atatactatc cttaccacca ctaaatgaca
    12001 ttacaaattt ttcatcagcc aattttaaca cctctttcat ctaactttgt tttattaaca
    12061 taattatacc aaatttttga gttgcatagc aaattatatt aaattgttaa ttcactataa
    12121 agaatgtccc taatattaaa agtataaccc ctatgtattt gtataaatgc attgtttctt
    12181 gtcccaaaat tacgctatca ataaaaaatc caactaaaac ttgggataca agagttaata
    12241 taagtgtatt tgatactcct aaatttggaa ttgcctttac tgtaaaatat attattagac
    12301 ctccaaatac tcctcctaat aacaacttat aattaaaacc atttatagat actagacact
    12361 ttaagtcacc tgtaactatt atacttgcca aaaaaaatat tgctcctaca gttaagctta
    12421 taaaggtggc cactaatgct gttgtacttt ttcctagctc tccatttata aaagcttcaa
    12481 gagctgtaga accacctgct aataatgcaa atattattgc ccataatttt gtcatggaca
    12541 aacctccttc tatataattc tattaataga agtttatgat acaacctatt aattaattac
    12601 taagtatagt ttaattttta tacactaggc tttatttgga tatgaatttc tgttataatt
    12661 gttctatcga ttttggaggt gattttatgt acacaaattt taatatcgat tttaataact
    12721 ttaataaaaa agaaaatgct attaaattta tgttaatggg agtattactc attatattag
    12781 gattattatg ccttacattt aagacattag gtataaaatt aatttcttgg acttttggta
    12841 ttgcactttt attctttgca tatttaaatt taaaaaatat taatgagtta aaaagatatg
    12901 ctactaaaga agaaattaaa ccctctatta atatccaatg ggttctaata attgcatgta
    12961 tattactttt tgtatttcct caaaaaatac aaagcatatt ttcattattt ttaggatttt
    13021 atttaatctt taatcaacta gtagcacttg taaatagtaa gaataatcct tattctaaat
    13081 ttacaacttg gaatatagtt aagattctat ttggtatatg ccttatatta tctccacttt
    13141 ttttatctag atttatagta tcgataatgt cttttttcat aatattattt ggactagttc
    13201 tatttttctc tggaaataca gcgagaaaat attaattatt ttctcgctgt atttttatat
    13261 tacttaaaat agaatttgtt taagttatct atttgtggta atatgttttt tgtacaagct
    13321 cagcgtacaa ttttattaga aataggtgat tttttggata aatttaaata tgcttttgat
    13381 aataaaagat atcatacttg gaattactat cttagaaata cttttggaga aaaagttttt
    13441 aaggtatcta taaatgcagg atttacttgc ccaaacattg atggaagtct tggttatggc
    13501 ggatgtacct attgtagcaa agaaggctca ggtgattttg ctggaaaccc taaagataat
    13561 cttatcagtc aattttataa tataaaagaa atgatgttaa aaaaatggcc tcatgcaaag
    13621 tatattggct actttcaagc ctatacaaat acgtatgctc ctcttgaagt tttaaaagaa
    13681 aaatatgaga ctatattaga acttgaagat gtcataggtc tttcaatatc cacaagacct
    13741 gactgtttgc cagatgatgt tgtagaatac ttatctgaac taaataaaag aaccaattta
    13801 tgggttgaac ttggacttca aacaattcat gattctacat ctaaaattat aaatagaggt
    13861 catgattata aaacatttgt tgaaggtgtt gaaaaactaa agtctaaaaa tataaaggtt
    13921 gtcgtacaca ttataaatgg tcttcctggt gaagattata atatgatgat ggaaacagca
    13981 aaggcagttg gtaaaatggg tgtagacggt ataaaaatcc atctattgca tgttataaaa
    14041 gatactccta tggaaaagat gttacaaaat ggtatgttaa ctcttatgga acaagatgaa
    14101 tatatcaatc ttgtctgtga ccaattagaa atacttcctg aaactatgat aatccataga
    14161 ctcactggtg atggtaagag ggatgaacta gtaggtccta tatggagcct aaaaaaatgg
    14221 gaaattttaa atcaaattga tgatacttta aaggctagaa attcttatca gggctgtaag
    14281 tttgtttaat acaaaataat aactatagaa atggttatat aaatatagtc ctttctatag
    14341 ttatttagta tttacaaaac aaaatattaa tttgtacatt tatacaatat ataataaaaa
    14401 atagatagtc taaactgtat ctacttatat tttatttgta ctgtccactt tattctataa
    14461 aatttataaa tagttatttt ttaataccaa atataatttg gtataatttt tgttggactc
    14521 taaaatgtat accaataaat cctttttaag tatataattc taaatacttt cagttttttc
    14581 aatctgcttt aacttgttgg acttataaaa tattcttatt tcatcataag aaagattaag
    14641 tgctactact gcagatatta acatcaacat cactccaaat aacttcccta aggaaaaact
    14701 ttctcctaat atagtgcaac caattattac tgaaccaact aattctactg atgccacaac
    14761 acctgcaact gacaactcta cacctttaga tatacctgct gcataaaaaa tatatgcaag
    14821 tgcagctgga acaatgccta acattagcat acaagataaa acatctaaat ccttagcata
    14881 atttaagatt tcccaaggtc ttgaagaagg aatcataaat atagctccaa ataagaaact
    14941 gtatactaat attgttgaac ttgaaaattc ctttaaagca tttttactaa tagttggcat
    15001 aagtgcatat gctattgctg ataatacccc aagtaaaagt ccaaaagcgt ttaaaccttg
    15061 aaaatccaaa cgtccaccag tcactgccat aattgcacca ataaaacaaa gaataagtga
    15121 aaataatttg cttctagtaa tattttcttt ataacatatt tttgaaaata ttgctaaaaa
    15181 cagtggtgat gtatatagta aaactgctgc aattgataca cccgcaatgt caattgcttt
    15241 aaaataacat aaattaaaca ttgcctgaca tataatacct attattactg aatatataat
    15301 tccttttttg cttatcttta agatttgtgg tgtccttata gatgaatata cgcctaaaac
    15361 tataaatcca aaaaacaatc ttgtaaatgc tacttgttct gatgtcagtc catttcccat
    15421 taaaaacttt ccaaataatc caagtgtagc ccataataac cctgctattg ctataaatac
    15481 atacccttta cttttttctc ccattttaaa ttcccttcta tttttattta gtcaatttac
    15541 actcttcttt ttaagcaaaa actatgccat cttaaatcaa tatatatcga ctctaaaacc
    15601 ctagtctttc caataatact aataaatttt caattttttt atatttattt ttcacttact
    15661 ttaagttgat ttagacttat tttaattgta aaatgagtta ta
//
LOCUS       DAMKMI010000095        15555 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMKMI010000095 DAMKMI010000000
VERSION     DAMKMI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15555
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.098"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(44..904)
                     /locus_tag="PC429_002609"
     CDS             complement(44..904)
                     /locus_tag="PC429_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF3848107.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1221..2549)
                     /locus_tag="PC429_002610"
     CDS             complement(1221..2549)
                     /locus_tag="PC429_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF3848108.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2697..3026)
                     /locus_tag="PC429_002611"
     CDS             complement(2697..3026)
                     /locus_tag="PC429_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF3848109.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3050..4339)
                     /locus_tag="PC429_002612"
     CDS             complement(3050..4339)
                     /locus_tag="PC429_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3848110.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4355..4681)
                     /locus_tag="PC429_002613"
     CDS             complement(4355..4681)
                     /locus_tag="PC429_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3848111.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4816..5445)
                     /locus_tag="PC429_002614"
     CDS             complement(4816..5445)
                     /locus_tag="PC429_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848112.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5661..8318)
                     /locus_tag="PC429_002615"
     CDS             complement(5661..8318)
                     /locus_tag="PC429_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3848113.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKHYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8677..9621)
                     /locus_tag="PC429_002616"
     CDS             complement(8677..9621)
                     /locus_tag="PC429_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848114.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9614..10639)
                     /locus_tag="PC429_002617"
     CDS             complement(9614..10639)
                     /locus_tag="PC429_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848115.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10714..12282)
                     /locus_tag="PC429_002618"
     CDS             complement(10714..12282)
                     /locus_tag="PC429_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3848116.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGVFWNIADWTIE"
     gene            complement(12395..13306)
                     /locus_tag="PC429_002619"
     CDS             complement(12395..13306)
                     /locus_tag="PC429_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848117.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13290..14276)
                     /locus_tag="PC429_002620"
     CDS             complement(13290..14276)
                     /locus_tag="PC429_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848118.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14636..15037
                     /locus_tag="PC429_002621"
     CDS             14636..15037
                     /locus_tag="PC429_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3848119.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15040..15438
                     /locus_tag="PC429_002622"
     CDS             15040..15438
                     /locus_tag="PC429_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF3848120.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
BASE COUNT         4902 a         2724 c         1725 g         6204 t
ORIGIN      
        1 ttgtattata taataagtat ttttaggatt ctgttacata atattattta aatattcttc
       61 tatatttctc ttcttcttct tcagtaaaca cattaaatac aacttttaaa tttttttcag
      121 gattttcttt tagccatagt ctaactctac ttacagcaag attagctgcc tctttttttg
      181 gataaccaaa tacacctgta gaaattccac aaaaaacaat attttttatt tcatcaattt
      241 cttttattgt atttaaacat gatttataac aatgaagcag ttgcttttct tgctctttac
      301 ttggctgacc accagataca ataggtccaa cagtatgtac tacaaatttt gcaggtaaac
      361 aataacctct agttatctta gcatctccag tatattctaa atgcccttgt ttttttatta
      421 ttttgtcaca atcttctcta agtctaggac ctgcgcatga atgtatctca ttatccacgc
      481 atagatgtaa tggttgtaaa caccctaata acttgttatt tgcagcattt actattgcat
      541 ctgctcttaa attcgttata ttacctctcc atatggcaat cccttcctct acttcttcta
      601 tatcatttac atctactaac tctctttctt cacattcaat caacaataaa ttatgtaaca
      661 tattaagtat ctctggagca atttcttttg gtggatatac attcaaaagt cctctaagta
      721 tatctctagg cttaatttca tcatttgata aattgtatat tttagaagga acttcttttg
      781 aaaaatatgc aattaaagtg tttatatttt ttcttctttg ttcgtcagtt agaggcttga
      841 tagttttatc aaaatcctca aataaattta catcatcagc ataatctctc cacttcatag
      901 ccatctctcc attcttgtat tttagactta aaaataatta tgtatttatg tacatattta
      961 tttatttcta taaacaccta cataaatcct gttaaaagtg taaatttttt attatacaac
     1021 ttctctggtt atttctattg attgatagta aattattgta cctaaattta atccaactaa
     1081 caagtaacta aacctacttt cctaactgca aaatacacta tgtttgccac ttgtatctca
     1141 tttgtagata aataaagtcc ctacaaatac attaactagg ctatgtacta aactgtagga
     1201 actttaaaaa tatattattc ttaattaagt tttggccaat aatttttatt tgcttctatt
     1261 aaatcatcta gtaattcttt tgcaactgtt gcactaggaa ctgtcttaga tagcataagt
     1321 gcttgccaca atttattata agacttctca atccaagcat caactactaa tttttcaact
     1381 gctaattgtt gttctattaa acctttttgg aatgttggta ttttccctat tgataatggt
     1441 tcatacccgt cttttccaac tatacatgga atttcaacca tagcatcatc ttgtatattt
     1501 tctatagctc cattattttg aactatcaat aacatacgct cctgtgtatt aaaagctaga
     1561 gctgtagcta aatccactat aaactctgca tgtattccta tcttaagccc actgccctta
     1621 gaatgtcctt gcttttctat caatgcacat gaaccaaaaa cttctttttc tcttccatca
     1681 ataacttcat tggcacgagt ataatttaca tcagaatgat ttacaacata atctggaaat
     1741 agatagtact ttaagtaact acttggtatt gtagttaaat ctgtttttac tatttcttta
     1801 gtaaataaat tagtttcaaa ccaactatct ttcttgtcat ttttcattcc ttcttcacca
     1861 tttataaaac catattcttt tatatgttct actaattttg gtaaaagttc atttcctttt
     1921 ttatctttaa ttgaagtata ccaaccaaag tgatttaatc caaagtatct tatatccata
     1981 tgtttacgag atttgagtcc tgcaatcctt gctatattat gttccatacc tattggcata
     2041 tcacaaatat ttattaccct agaattaggt ctcaaccttc taacagcttc cgctattatt
     2101 gatgctggat tagaataatt taacatccaa caatttggag aatacttttc catataatct
     2161 atgttttcaa gaacaccttc tatagacctc attccataag ctattccacc tggtccacaa
     2221 gtttcttgac caactacccc atgttttaga ggtatctttt catctagttc tctcatttca
     2281 agttttccaa ctcttatatg agctaaacaa aaatctacat ctgtataagc ctctttaggg
     2341 catgttgttg ctaaatactc tatattaggg tccttttcct ttattagtat ttcacaagct
     2401 ttagctacct tattttgtct ttcttcatca ttatcatata atttgattgc tcttaatggt
     2461 aacctatcta agttatctag caacattaat atgatttctg gggtatatgt acttcctcct
     2521 ccagctatta ctactgaaaa tttttccata ataatatctc cttttctatt taaaaattta
     2581 ctgtatttat aaataaattt ataaatacta gtactttaaa gtaaaaggga gttcatttat
     2641 taaatctatt gatttaataa actatagcca aacttcaagc actatttaca agaaatttaa
     2701 ttcttattaa ttcttgggtc catcatctca aatatatcta caagttcaga tactaaagaa
     2761 tgagcaaata atgcatttga ataatggtct tcagagtgca caactaaaaa atcagtccca
     2821 ctatcttcac tattcatagc atttgatagt aattctaaat gatgctttga tgctgtttgt
     2881 attttctctg aggctgaatt taaaaggtgt tttgctttat tgtagtcacc tttttttatt
     2941 tccttaagag acatcattac atcactttta gcatctcctg cttctgatat tatactaaat
     3001 actttttctt ccatttcagg taccatatcc ttctccttct aatgacttgt taaaaatcaa
     3061 agctatcatc atctaatgtt gtagctaatt ctgcattttt atttaattct tccttctcca
     3121 ttatcttaaa gaatggataa tatatcaagg tcataagtat tatacttgca aatactaata
     3181 cgattgcctt ccaatctaat gtcatcaaaa atgctgcaac ccctggaggt aaaaagcctg
     3241 gtggttctgc aataggtaat cctactaatc ctacttttaa tgccataaaa ttaagtgctc
     3301 caaccaatac aggtccaaat acaaatggta caaacatata tgggttcata actattggtg
     3361 ttccaaatat taccggctca tttattccaa atagtgatgg taaaatcaca acttttccca
     3421 tactattaaa actcttacac ttacttcttg acataaggta cactaatccc aaagttattc
     3481 ctgaaccagt tacttgcaag aaaccatagt aaaatcctat tgtaaacaca tattttgggt
     3541 ctcctcctgc tgctatcagt gttgcatttt ctgccatata tgtagctgct attggcatcc
     3601 atatacttga agttattgct gctccatgaa gcccaaagaa ccataataat tgagttataa
     3661 atagtattat agttactgca aaaacattat ctacactgct tatagctggt gctaagaaat
     3721 tcattataat agctggtagt ggttgacctg agacattttt agttattgct gctattgctg
     3781 taaataacat tatacatact atcattggag ttaaatttct aaaactttta tttactacac
     3841 taggtacttc ttttggaaat ctaattacga gatttttatc attcataaac ttagtaatat
     3901 caactactaa aagtgctact aatatactta caaataaacc ttgtgcatct aaataacttg
     3961 tatctaatac accatctact acttgtccac atacaacaag ttgtgcaaca actgctgtca
     4021 tgcctatttc tacatgatta agattatatt ttttagctag tgcctgagct ataaaaaatg
     4081 ctgaatatat agataataaa ctcatagtaa atttattagg gataagtaat gtatccatat
     4141 tttcagataa taatgttttt gcacttcctt caggtaacat attgtttaat gccataaata
     4201 acatacttaa agaaccaaca attattattg gggtaatgct tatcatacct tcttttatag
     4261 cttgaagatg cctttgtttt tcaatctttt gtgctattgg aagaagaaat cttcttaaat
     4321 tattttgaag tgcttccatt tttattcccc ccaattatct atttttatat aattcgattg
     4381 ccgacttcat aacagaagga gcatccatac gaccataatg cttagtatct attaaaccaa
     4441 ctgctatttt gtaattctta caagcttcaa ctacatcatc ttccttaaac ctaatttgtg
     4501 gtgctacaag tactacatct gtatcattag cttcaatgta tgattttaaa ttttcaaatg
     4561 atgttgcctc tatatttata gttttagttt ttaacttttc atgctcacta caaaaacttt
     4621 tcatcttttc cacaagtaca cctgttgaag ctccatttgc acatactaat aaaatattca
     4681 taattaccct cctacaatat ctaacctctt ttattttcta attttttatc agccaattct
     4741 tactctaatt cctatcagtt tttactttaa ttcttattat tttattttaa ttcttattaa
     4801 tatttactct aattcttagt actgccaatg attaaacttg aaagccgtat ctataacttc
     4861 ttcttgctta tatttttcta atatttttaa gtatctttct ttataatcat tatctacttc
     4921 tttttctggt attactatac tagcttcatt taaaaagtgg aatgaatccc ttcccatttc
     4981 taatcctcta tatgtatgct tgtcaagtgc cacgtccata acttctggaa ctttacccat
     5041 tgcaaatcct tttataatta tattttttaa taaatcactt gatctatctt tctcacatgc
     5101 acaaagatat cttattgcat gtatgaaaaa taaaggtctg tctccatctg catatgaata
     5161 ttcctttctt acttgattta aattatttat gattattaca gctgatggat cccccattcc
     5221 tatatcttca actgatattg taagtatcct tctccatagc ttatcttcta attctggtga
     5281 tgatatatat agttcataag caaattcaca agcttcttcc accatatttc ttcttatagc
     5341 tttttgaaat gacgatatta tttcgtctgc actaaatccg tttcttgtag tcattcttgc
     5401 ccatgggtca tttgcctttt tatctcttaa tattttatct tgcatattat ttactccttt
     5461 ttatttaaat tttaattatt tatattactc atttaataat ataagcaagt attatgccag
     5521 tttttttaga cactttaaaa cactgttatt tcaatcattt ttatcatcaa aacttagata
     5581 ctttgtctaa gtatatttat atgtgtctaa tattaatatt attttagact aaaaaaaaat
     5641 taccaaataa gtttttatac tcattggtaa tctttttcga ttatttctgc atattctaat
     5701 attaataata tttgatagca ctctttttca ttaatttcta tattatattt tttatctaat
     5761 tgagatacta tatcttttat tttgcttaga ctcttgtgat aatcaagtgt tttttcttta
     5821 tcaagctttt caatttctct agtatttcca atcaacccat ctaccatgaa agctaaatgc
     5881 atcataagtc caattacttt atcgtatttc ttttctatat taaatactaa aaacaattca
     5941 tcagacacat attttatatc tttaaatatt ctattataat tagagctttt taaggatgtc
     6001 tttaaacctt caactacatc atcataacct atacttatct ttataatatt ttttaattta
     6061 ttgatatttt catccttatc tatatctttt ttgctaatat agtcaattcc tctaatatta
     6121 attctttgtt cattgattac tgcaacaata ttactacttt ctttaagttt cgtaatattt
     6181 aaacttaaat ctttttctgt tttatatggg acagatataa tatctattcc atctaagtac
     6241 ctactcatct tttcttctaa tatttttctt gttttattat ctacaccttc tgaaacatgt
     6301 gatgctataa taattgtttt agacagattt tctttatttt gaatttcatt agttccaaaa
     6361 tagtgctttc tcgtgttagt gatagagttg tatacgtcat ttaaaggtaa tcccatattg
     6421 gccttctgaa ctgcttctaa cacagttaat gtcgtcacca taggaattac tcttactcca
     6481 cacttttttt ctttctttat aatttcatca aaggagttta atgaacccat atctgtaaat
     6541 agtaatacat ctttattgtc tatattatta attaaatttt taaaatctac aataacttca
     6601 tcatagcttt tatctaactt catatcgaaa gaaatagcac tgcactttcc aataatatca
     6661 ccaactgctt ttaccatact acttgctgta gtttctccat gcatagcaac tataatatta
     6721 atgttattca ctattttatt ttgttcatct tgtttagctt gtactatgaa taatgtaata
     6781 tatgctacag tttctataga taaaaataaa tcaaattcat tttcaattat tccaactatt
     6841 tttaatgctg ttttaaattc acttctgtta tttcttctaa tttgattcaa atctatgttg
     6901 tttgtagagc tacttttatc caatacgctt tttatataaa ttgccattga cttaaatatt
     6961 gattcactta tgtctatttc ttcatcttct ttaataattt gtcttattga ttcacacatg
     7021 cttttaaact taacattatt aaatagagaa gcatctgcta tattcatttc tacagattta
     7081 ttaagattag tatttactgc attaataata tttatatcat tgtagttaat aacttcttct
     7141 tctcctaatg acgtaaatac aaaatattct attccatgtg ttataacttc atctgcttta
     7201 atagacttct ctctataatc atcactcact tcatttatag atttatcaaa atattttttc
     7261 tctatataaa ctggttcttt attatctctt ttatatctta gatatccatt tgcaacagct
     7321 aattttatat cattttgaag ttgacctaca ttatttttac aatcataaag taatagtgat
     7381 ttaattattt ctttatgtac ttttattgaa atagaaatac tttttgattc ttccttgaaa
     7441 aaatttttga ctaattccat tctttcaatc attgtcctat ctctcaatga tggtatttct
     7501 attttcattg gtattcttct attgaatgta tctaatagtg ttgatgtcgg attttctgta
     7561 gtagcacata ttattaatac cttagactct ctttctttcg ttgcatctcc tagtctttta
     7621 aatgttccct tatctataaa agtaaacaac atttcttgac cttctgctgg tagtctatga
     7681 atttcatcta aaaatagtat ccctccattt gcaatttcca aaagaccttt tctggaagat
     7741 tctgcacctg tataagctcc ttgttctacc ccaaaaatat gtcccataag taattgtgca
     7801 ttattagcat aatcagcaca attaaatgtt acaaaaggag ctttattttt tctaattcca
     7861 ctatctatag aataatcgta catatatcta gcaatcatac tctttcctac tcctgtttct
     7921 ccatagataa tagtatgaag tccatttgta ggatagagca ttgcgacctt gcatttttct
     7981 attataggtc ttaaactatg ttctttacct attagtgttt caaaactatc tttttttatt
     8041 ttgttttcta cttttgttcc tttttttatg ccaagcaact cactttgtgt atcaagcaat
     8101 tcattcttgt taaggttaag atttgcaggg atataataca caggtcttcc ttttatcttc
     8161 gtaaccatac cctcattgaa taattggttt aaatatgtac ttatattgtt tcttgcaatt
     8221 cctgtctttt ctgataattg gttagctgta atcccttttt cattttccct atatgatgat
     8281 tctaaaactt tcattacttt atctttttta tatatcatta caagctcccc tacattatag
     8341 tattttatta actaaaaccc ctatacacac tttatttttt aataaactct tattctttaa
     8401 ctaaggttat aatttatcaa actttcctta ctttaaatta tagaaaatta ttgtagaaaa
     8461 aataagatta ttgctcattt tttctacatt actattttta attttatagt actttcttaa
     8521 caacatactt tacttgtttt taattttatt atatttgaat ttattatatt tgaaaagtag
     8581 aaataaaaaa ccctattttg gagtagtttt aaaatttctc caaaaatagg gttttagttt
     8641 gtatttcaaa taaatacctg ttcaagtatt tttatactaa acaactttta aattttgttg
     8701 atatttattt atttctgatt tagaagaata tacaaaatgt ccatctgaaa cctcaatcca
     8761 acttaatgac ttttcatttg aacaatcata tccattagaa ttatactcta ttctatttct
     8821 tgacttagca tatctagggt caggaacagg aattgcagaa agtagtgatt ttgtatatgg
     8881 atgaatagga ttattataca attcatttga atctgccaat tctactaatt ttccattata
     8941 cataactcct atacggtcac taatatgtct caccatagat aaatcatgag ctataaataa
     9001 acaagttaaa ttcttttctt gttgcaattt tttaaataaa tttactattt gtgcttgtat
     9061 agacacatcc aaagaagcta taggctcatc agctattata aattctgggt ctactgaaag
     9121 agctcgtgct atgccaatac gctgtctttg tccacctgaa aaatcagatg gatacttatt
     9181 tgcataactt ctatcaagtc ctactagctt tagcatctca tatactttat tcattctatc
     9241 tgttttattt ttacagatac cttgaatttt tagtggctct gaaattatat ctgcaattgt
     9301 catacgtgga tttaatgatg aagtagaatc ttgaaaaata atctgcatac ttttgtttac
     9361 atcttttttt ataaatttgt acgttttttt atctgatatg caatttcctt tgtaaattac
     9421 ttctccacca gtagattcat gtagtcttat tatagtttta ccagttgtag attttcctga
     9481 acccgactct cctaccaatc caaatatctc acccttatat atatcaaaac ttatatcatc
     9541 aactgctttt acagtagtac ttttgtccaa atgaaaatat tgctttaagt tttttatttc
     9601 cagtattttt tctctattca ttagaaatca cccttccaca atttactcta actggtacat
     9661 ctacttcagg tgcatctggg tgcaataacc aagttgctgc actatgtgta tcattaattt
     9721 taaacattgg tggctctttt tcataatcta tcttaagtgc atttttattc cttattgcaa
     9781 aagcatctcc ttttggtggg ttcaataaat ttggaggcat tccaggtata ttatataaat
     9841 agtcatcctg cgaatctaaa gtaggtaatg aacccaacaa cccccaagta tatggatgtc
     9901 tagggtcata aaatatatcc tctacagttc ctatttctac aatctttcct gcatacataa
     9961 ctgctattct atcagctata gttgcaacta ctcctaaatc atgagttata aatattattg
    10021 acaaacctat tttatgttgt aaatccttta ttaaatcaat tatttgagct tgtatagtta
    10081 catctaaagc agttgtaggt tcatcagcaa tcaatacatc tggattacaa gcaagtgcta
    10141 tagctattac tatacgttgt ctcattccac ctgaaaaatg atgtggaaat tgtttaaatc
    10201 ttttctcagg attatcaatt ccaaccaatt caataagctc tatggctctc ttttttgcct
    10261 cacttttact tataccttga tgaatgatta cagcttctgc aatttgcttt cctattgata
    10321 tagttggatt gagagatgtc atagggtctt gaaatatcat tgatatattc tttcctctta
    10381 tatcttccat ctctttttca gacttcttaa ccaaatcact tgatttaagt aaaacctctc
    10441 catttttaat atatccattg tatgggagta tcctcatcaa actcttacac agtacagatt
    10501 tcccacaacc agattctcca actatagcca atgtctcacc tttttttaga ttaaaagata
    10561 catttcttac agcctgaact tctccttctt ctactttaaa acttacagat agattattaa
    10621 cctccaataa atgttccaaa aaccatcaac ccctatgcta ttcaaacctt agggatatat
    10681 ttttatccct aaggtttcta attaatttca gtattattca attgtccagt cagctatgtt
    10741 ccagaataca cctacaccat gatgacctaa aactgtatca ggtgttaatc cttttatgtt
    10801 tggttttcca acatatattg catctatata agcaataaat gtataaggca tatccttagt
    10861 catttctact tggaattgtt tatataactt taatttttca tctttatctt ctgtttctct
    10921 tgctttttga agtattttat ctatagtagg attagagtaa gcactgtaat ttgcaccttt
    10981 atctgttcca aatactttat atgtatggtc atctgggtca aatggacttc cccaacctat
    11041 taaatgagca tcttgattag cccaatctgt ttcagttaca acaacagctt tagcatcaac
    11101 accaatttct tttaactgtt gtgcacatat tttagccata tcaactctaa cttggtcact
    11161 ctcacctgct gttatctcga aagctaactt agtaccttct ttttcataga ttccatcaga
    11221 acctaatttc caacctagct tttcaatttc ttgtttagct ttttctggat tatattcaaa
    11281 cttttctata tcaggattat tatatggtcc catttgtaaa ggtgaatatg ctggtacacc
    11341 atgtcctaat aatacactgt caactattgc ttttctatct attgcatagc ttaaagcatt
    11401 tggtaatcct ttagcttttt tatctttaaa gaatttagaa ttaaaattgt ataatatacc
    11461 tctatagtcg gctgttttca ttatatttac tttaaaattc ttttcgtctt tttcaaagtt
    11521 agacatatct ttaggagtta tttgagctag gtctaattct cctgatttta attgcattgc
    11581 cttagcctta tcatctggaa caatcttaaa gactacttta tctaatcctg gttcttttac
    11641 aaaataatca ctattcttaa caagtgttat actttgacct ttgtcccatt tttcaagttt
    11701 aaatggtcca gtacctattg gtttttgatt aaattcatca gtagctatat ccttaccctc
    11761 caaagcatgt tttggtaata ctcctactgt taaataatca agcattgctg tatttggagc
    11821 ttttaaagta atttttatag tattatcatt aaccacatca atttttgtta tatcttcata
    11881 atttgatgct atctcagaag cattatctgg attcattata gtttctattg taaacttaac
    11941 atcatttgct gtaaatttct ctccatcatg ccacttaaca tcatctctta aattaaaagt
    12001 atatgtattt gttgcctcat caaatttcca gtcctttgct agacaaggaa ccaccttatt
    12061 attttcatca tgagctgtta atccattaaa tattaacgaa tttatttctc catgttcata
    12121 tagtgcagga tttatacttg tatagtcatt acttccataa acaagcacct ttccatcttt
    12181 aggcgtatct gccttcttat ctttgtctcc tccactagaa caacctgcca ttagactaag
    12241 tatcattact aaacttaaaa cttttaactt ctttaatttc atgatgctcc cccttttgct
    12301 ttttataaaa tatttttcaa agctttgacc ccatttattt aaatagatat ttcaaatacc
    12361 tcagtatttg cttatttaat actcaaatgc tgatttataa attactagat tttttattat
    12421 tattctttct tatataatta cctatatttg ttatacacac caatgtggta accaaaaata
    12481 tacccggaat cagtataatc caccatctat ttgaaagtaa tgcttcctct gacaaagata
    12541 acatacttcc ccaagaaaca atttctattg gtaaaccaat tcctaaaaag ctaagtgtcg
    12601 attcagtacc aatagctgcg cctatgtttg ttacaaccat gaacattatt gatgccacaa
    12661 aatttggaag taaatgttgc tttaatacat agaagaatcc tcctcccatg ctcttagctg
    12721 ctagtatgta ttcgctattt tttatttgtc taacttctgt tctcactatc ttagatatat
    12781 tcatccaact tgttatccct attactatgg acatagatac tggatttgaa tttccaagta
    12841 tagcttgtac aaatattata attaatattg aaggtatact aagtatgatt tctgtaaacc
    12901 tcatcattgc atcatctacg agttcagatg ccgaaccact tatactacca taaacaattc
    12961 ctataacagt tgatattaca gtggaaaata gtcctataaa taaagatatt tttcctccat
    13021 accatatcat agaatatata tctctaccca ttgagtctgt tccaaataaa aaagttttat
    13081 ttggagctaa attagaactt gctaaatcca tgtatgttgg ttcatgtgtc attataaaag
    13141 atgagaatac acttccaatg actatgattg ataaaataat aattgatata tatggtaatt
    13201 ctttaatttt tttagataag gttttctttg tttctatatg ttcatcttct gctactaaaa
    13261 tataattttc tcctacaatt tcaaaatcac tatattgtgg cttcattagt atcacctcta
    13321 tcatatttca ttctagggtc tattttattg tttataattt gagctaacat attcccaaat
    13381 actactaaag cccctgttat taagcataaa actaataaca tattataatc atgatacttt
    13441 gcactttcaa aacaaagtgt tcctaaccca ggataagaaa aaactttttc aactacataa
    13501 gttcctccta gtatatgagg tatcgaaatg gccattatac taatatatga tggcattata
    13561 tttctcaggc aatgcttaaa aacaatagtt ctattattta aaccttttgc tttacataaa
    13621 agcacataat cttctcttat ttcctctagt aatttatttc ttatcatata tgtgtaatac
    13681 catagatggc ttaatataag tactgtaaga ggtaagatta gatgtgatat cctacttaat
    13741 atactggact cttctcccat tgaataagca ccactacttg gaagtatact taagtttata
    13801 ctaaatataa gtatgagtac taaggcaacc caaaatgatg gaatacagtt ggttattgtt
    13861 cctaatttgc atatgattct atcaatgagc ttatcttcat gtaatgtaca aaatattcct
    13921 aacaataagg ctagcacaaa ggtaagtata tatcctgaac cacctaatat tatagtgttt
    13981 atatagacat catttataac agaagtaaca ttttgtttat atttaaatga aattccaaat
    14041 tccccttttg atgcatttga aatccatttt atatattgac tgtatattgg ttcatccaaa
    14101 ccaagttttt tcatagcatt ttctttttct tgtgtactca ttctttctac tccatcccca
    14161 tagtaagaga ttaatgggtc tcctggagca agtcttgcca tgtaaaaaac aataaaagat
    14221 aataaaaaca ttactaaaat aaactgcaac aactttttca gtatatcctt taacaaaccc
    14281 caacccccct ttgttgcaat aaaaaaaacc acataaaagc aaacgtaaag taacgttaaa
    14341 accttcgtgg ttccattatc attaaacaaa attaaatttt ctatatttat ctatgagtta
    14401 attttagcat catttattaa ctgtgtaaac aaacttttta aaaattgtta aaatttcatc
    14461 aaattatttt atttatacct aaaataaaat ttttatattc tattcttcat tattttctat
    14521 aaaattataa aattttaggt tgactatata agaaaagtat attatattag tttgtatgca
    14581 aaccatttaa gaggtgattt tattgaaaag taattatgac tgttttagaa tagccatgct
    14641 cttaaaagaa ctttattcta aaactatgta cacagtagaa gaaaatttta aagaaaatgg
    14701 attaacacac caacagatta tagttataaa actgatagct cataatcaag aacttacaat
    14761 atcacaacta tgtgatgaaa tgtctttagc taaaggtacg gtttcaggca taatcagcag
    14821 attagaacag attggatata tagaaaaatt caagaaatct aatgataaaa gaaatacata
    14881 cgttaagttt acaacaacag gatttgaatt tgccacaaat tttaaaatta aaatgcaaga
    14941 aagttttgat gatattttta aaaattgtga tgaaaacgaa ttgagtgact tagtaaaaaa
    15001 tcttagaaat atattggcaa aagtaaagga gagataatta tgaattataa attaatactt
    15061 gcaatagtat ttataacatc agctttaatt ttctacacta taggtgtatt tggagaaaga
    15121 aaggcaaaaa ttttaaaaaa gaaacatgta attatatttt ggcttggttt tatatttgat
    15181 actttaggaa catttacaat gagtaacata gccaacagcc atacttttga agtaaaatct
    15241 gctttatcac aaaaccttca tagtattaca ggtcttcttg caattgtgct tatgcttttt
    15301 catgcaagtt gggctacttt tgtcctatac aaagatgatg aggaaaagaa aaaattcttt
    15361 cataagttta gtatagttgt atggactata tggttagttc catattttat aggtatgttt
    15421 attggtatgg ctggatagta aaataaaaag tgtgtaagaa atactgtagg taaatttgta
    15481 taagacaaat ttatctacat ccttctttca cacttttaca tatttattta taatactcca
    15541 ttatttcttt catgt
//
LOCUS       DAMKMI010000096        15503 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMKMI010000096 DAMKMI010000000
VERSION     DAMKMI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.099"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            57..908
                     /locus_tag="PC429_002623"
     CDS             57..908
                     /locus_tag="PC429_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF3848121.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1041..2654)
                     /gene="ggt"
                     /locus_tag="PC429_002624"
     CDS             complement(1041..2654)
                     /gene="ggt"
                     /locus_tag="PC429_002624"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF3848122.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2879..4816)
                     /locus_tag="PC429_002625"
     CDS             complement(2879..4816)
                     /locus_tag="PC429_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848123.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKIIELIDKDINIEMYYKNRNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4803..5570)
                     /locus_tag="PC429_002626"
     CDS             complement(4803..5570)
                     /locus_tag="PC429_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848124.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5858..6532
                     /locus_tag="PC429_002627"
     CDS             5858..6532
                     /locus_tag="PC429_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848125.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6556..7560
                     /locus_tag="PC429_002628"
     CDS             6556..7560
                     /locus_tag="PC429_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3848126.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7672..8901
                     /locus_tag="PC429_002629"
     CDS             7672..8901
                     /locus_tag="PC429_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF3848127.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8909..9583
                     /locus_tag="PC429_002630"
     CDS             8909..9583
                     /locus_tag="PC429_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848128.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9586..10818
                     /locus_tag="PC429_002631"
     CDS             9586..10818
                     /locus_tag="PC429_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848129.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11104..12369)
                     /locus_tag="PC429_002632"
     CDS             complement(11104..12369)
                     /locus_tag="PC429_002632"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF3848130.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12422..13570)
                     /locus_tag="PC429_002633"
     CDS             complement(12422..13570)
                     /locus_tag="PC429_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF3848131.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNKVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13670..14215)
                     /locus_tag="PC429_002634"
     CDS             complement(13670..14215)
                     /locus_tag="PC429_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF3848132.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14438..15382)
                     /locus_tag="PC429_002635"
     CDS             complement(14438..15382)
                     /locus_tag="PC429_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF3848133.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         5424 a         2417 c         1948 g         5714 t
ORIGIN      
        1 gttaataatt aatataaatt aaaataaaga taatataaac acaggaggta cattctttga
       61 aaaacattat ttcagcaaaa gaacttatat caaaactaga aaaaaatgat aatctagttg
      121 taatagattg cagatttgat cttatcaata gaacttatgg tattgattct tataaaaaag
      181 gacacatcaa aggcagcttt atcttagata tagacaagga tttatcatct cctacaaaag
      241 aacatggtgg taaaaatcct ttacaagacc ctttaatact aaaagaaaaa ttagaaaaaa
      301 tgggtgttga taacgacact acgattgtaa cttatgatga tggagattta aatggagctt
      361 gcagattatt tttccaatta aaacatttgg ggcttaaaaa tgtctatgta ctggatgggg
      421 gaataacatc tttcgtaaag gaaggtggag aattggaaga gaaagtgaat ataccaagtt
      481 gtacaggtaa ggaaattaga ccaaatataa acaatggttt agttgttcca atggaatatg
      541 ttaagtctaa attatacaaa aaagatactg taataataga ttgccgtgct aatgagagat
      601 accagggttt agttgaacct gcttactcaa aagctggtca tataccaagt gcaaaaaatt
      661 atttttgtaa ggatttaata aaaagtgact ttgaaaatgg ctcacttaaa gatatagaat
      721 ttttaaaaaa attctttaaa gatttaaata attatgatga agtaatttta tcttgtggtt
      781 cagggatatc tgcttgtgtt aacagtcttg ctcttagaga actggatata cctcacaaga
      841 tttatattgg tagctttagt gactggatta gttatgatga caatgaaatc aaaactggtc
      901 aagaataagg ctcataatct atataaaatc agcatatgat agcttaaatt ttgtaaaggc
      961 gtcagtcatt gtagactttc gcctttacta attatttcat atactcttta aatttattat
     1021 atatatttta gttgataata ttaataaact gcaatatgac catcacatct tgattccgtc
     1081 ccacaaatat aactttcctg ttctgtttta aatattattt gacctcgtcc catgtcgaca
     1141 gtatcatgaa ttacttttat ttcatgaccc ttttcaatta atgaatctat tatgtgcttt
     1201 ggcatctctg gttcaacttc tatttccttt ccttttatcc actgccatct tggagcatct
     1261 agcgcctctt gtggatttaa tccaaaatca atcatatttg ttaacacttg aatatgtcct
     1321 tgtggttgca taaaagctcc cattacacca aatggaccta ttgctttatc ttctttacct
     1381 aaaaatccag gtattatagt atgataaggt tttttaaatg gttttactac attgtggtgt
     1441 ttagggtcta aattaaaatt atttcctcta ctatgtaaag caatcccagt tcttggtatc
     1501 acaattcctg aaccaaaatt tatgtaattg ctctgtatat atgaaaccat atttccatcc
     1561 ttatctgcag tacaaaggta tactgtacca ccacaatatg gctctcctgc agttggatac
     1621 aaagcctctt tattgtcaat caataaactt ctttttttag catactcttg gcttagtaat
     1681 tcctgaatct ttaccttcat atgctctata tcagttacat atgttttaga atctgcaaaa
     1741 gctaatttta aactctctat aattttatgt atgtcttcta cattttctat atttccttct
     1801 aattccaatt cttttaatat gttaagtgcc attaaaactg ttattccatg accatttggt
     1861 gggatttcaa atattttata tcccttatat tctgtactta taggttctac ccatgttgga
     1921 taaaaacact ctaagtcact ctttcttata gccccattaa attttttaga atattcatct
     1981 attttatcag caagtcttcc tctataaaag ctttcagcct gagtatttgc tatctcttct
     2041 aatgtacttg cctgttcttc acatataaat atatctccag cctctggagc tttaccatct
     2101 ttagaaaatg tatcaaacca tggtttgaac tcttctccaa taagttcttt ttcatagagt
     2161 tcatatgatt ttttccatac cttagctaca ttaggagata ctacgtatcc ctctcttgca
     2221 tagtttatcg ctggagataa gcattccatt aagctaagtt taccatattt tttatttaat
     2281 tctgaccaag ctgctggtat tccaggtaca gttactgctc caaacccata ttttggcatt
     2341 tctttcatat tattataatt ttttaattcc atattttctg gagcaaagcc actagaattt
     2401 aaaccataca atcgtttttc ttcctctatc caaacaaggg catatgcatc tcctccaatc
     2461 ccattgcttg taggttctac tactgctaat gtggcggctg ttgcaactgc tgcatcaaca
     2521 gcatttccac ccttttttag tatttcaagt ccagcttgag atgctaatgg tgttgatgtt
     2581 gcaactgctc catttttagc atacacgaca tttcttattg atgagtattt atgttggtag
     2641 gcattaaact tcataaattt cagctcctcg tcatttttta tttgatattc tgcttagatt
     2701 tattatataa catcaattaa tgtaactgat acaaaatgat ataaaatata gactttttcc
     2761 taactttaat tttatcttta aataaatatc ttaaactcaa ttctagtatt ttctatctgc
     2821 acttaaataa gttattttat tcaaaacata tatctaattc ttttaactta aaaataattt
     2881 attcttttat catgtttaga gcatatttta aagttaaaaa ataatatata aaatatccta
     2941 taaaataaat gactagagtt atgataattg gtacaagtaa tgaccttggt ataaactttt
     3001 gtgctataga catagcaaat aaattatgaa ctgtacctac aactaatggc attaaaaata
     3061 taacttttaa ctgttttgat attattttct caatatcttt attatttgct ccaagtttta
     3121 ttaacataat atatctttcc ttatcatcat atatatttga taattgttta aataagatta
     3181 tactacctgt acataaaaga aatactacag acaaaaatat gcctataaac agcattagac
     3241 cacttaattt tatcaagtta tcataacttt caatacttga tgtaaaatta aaaggataag
     3301 taaatttcat attttcactt actatatttt ttaattttaa agtcaaatcc agttcttttt
     3361 tttcattttt tatatcaatt aatcttatta atctagaatt tccatgtaat tttaactgat
     3421 taaatacgtc atcttttact acaattaaat caaacgtagc ttgtgaattt atttctggct
     3481 ccaagatgct tttttgtact ttaaacagat ttatatcata ctcatttgca tctttaatat
     3541 gcaagtttat tttttcaatc tcaaagtctt taaaaaatat ttttttgtat gaatcagaaa
     3601 catagtacac agattccttt gaagttaatt cttcataatt gttattctga tattgtctta
     3661 attttttaaa atcactttct ctaataatat ctacagacgt tctatcttct atataactat
     3721 ttcgattttt ataatacatt tcaatattta tatctttgtc tataagttct attattttat
     3781 caaagattat cttattatct tttttatatt tatataataa tttgtcaatt tcgttattta
     3841 cataagaatt tcctaaattt atggtataac tgtatttata attttcactt atgttcctat
     3901 ctatatcgta ataaagtgat atggtaaacc ctagcatagt tacacttgta gctattaata
     3961 ttgctattac agccaataca ctactattgc tctttaattt aaatccaagt tcagaaaatg
     4021 caattaaatt ctgtccctta taataaaaac tttttctctt cttaacagca tcaataatca
     4081 tatacatagt tgatgaaaaa aataaaaaag ttccaggtat tattgtaact aatataaaaa
     4141 ctgaaagcat aatattttca gcaataaaat aactaattga cattaaataa gaaatagcta
     4201 ttaataaaac accaagtaca cattttaact ttataaattt tttaaactta tacttttctg
     4261 gatatttatt aaataactct ataattctct tatttctgat aactatagta tttcttattc
     4321 caataactat gaatattatt aaaaataccg ctagtgtctg aactaaggcc tttatactta
     4381 agttcatatt tactactatc atttcattca ttaattttac aagcatcata gtcataagct
     4441 ttgaaaataa cagtcctaaa aatataccta ttagtattgc aactataccc atcaaaaaag
     4501 tttcatataa aaatattttt cctatctgat ttttcctcat tccaagtaaa ctataagttc
     4561 ctacttctcg cctacgtcta ttgaaaaaaa atgagtttga ataatatata aataaaaata
     4621 caaataccac tattacaatt gaacttgtat taatactcat cctagttttc tctccaagtg
     4681 aactcaacgc ttcattatac tctatagatt taaaactaaa aaatacaaat acactaaata
     4741 caattgaaat aaagtacatt aaataattaa aaaaactatt ctttacattt tgcattgcaa
     4801 ttctaaataa agtcattttt attaactcct ccaattaagg aaactgtatt tactatttta
     4861 ttatagaact ctctgttact ttctcccttt acaatttctg tgaatattat tccatccttt
     4921 ataaagagta ttctattgca aaaactcgca cttatagcat catgagttac catcaatatt
     4981 gttttattgc tgtctttgtt taattttaat agacaattca acaaatccct agcagattta
     5041 gaatctaatg ctcctgtagg ctcatctgca agtactaatg atggttttgt tgctatagct
     5101 ctacatacag ctcctctttg cttttgtcca ccagaaactt cgccagggta tttatctaat
     5161 atagattcta tatttaattc ttttgaaatc tcttttattc tattttctat tactgatact
     5221 ctttcatttt ttaaagaaag aggcaacatt atattttctt ttattgtaag tgtatccaaa
     5281 agattagaat cttgaaatac aaagcctaga ttatcacgtc taaaatctgc caattcattt
     5341 tgcttcattt taatagtatt ctttccattt atataaatat ctcctgatga aggtaaatct
     5401 attgtagaaa taatatttaa aagtgttgat ttaccagcac cggaagaccc cataatacca
     5461 acaaattctc ctttataaac cttgaaagtt attcctctta gagcttcatg ttttgaacct
     5521 tttcttccat actccttttt tatattttca acagataata tttcttccat agttaataat
     5581 cacctttcat cacaattagt tatttatcaa ttctcacaaa tatattatat atattaaaat
     5641 ggcttttaaa catttaatta tattaattta aggttacatt ttcgtaagta ttaattatat
     5701 ttcttcaaat ttattgcaat tatgtttaaa atataattta tcaagtaaaa tatagttata
     5761 ttaatataat tattttaggt aataaggtag aaatatttta atttatcaag tagaatatag
     5821 ttatgttaat ataactattt tgaattagga ggtaagaatg tttaaaataa tggtagttga
     5881 agatgacgtc tcattgaaaa atatcatagc aaagtgtctt actaaatggg gacatgatgt
     5941 tcatcaaata gaaaatcttg aaaatataat agaagagttc aaaaactata acccagaatt
     6001 agttttattg gacataaacc ttccatttta tgatggtttt cattggtgta atgaaataag
     6061 aaaaatttca aaagttccta taatttttat ttcttcaaga aattctaata tggatgtaat
     6121 aatgggtgta aatttaggtg cagatgatta tatacaaaaa cctttttctg tagatgtctt
     6181 agtagctaaa gttaatgccc ttttaagaag aacttataat tttgttgata ataactcaaa
     6241 tcagattatt cacaatggtg ttactttaga tttatcaact gcaacaataa actatgaaga
     6301 taatactatt gagctaacaa aaaatgaaat caaaattctc catgagctta tgaaatataa
     6361 ggggcagata gtaagtagga ataaactcat gaaaaaatta tgggataatg attggtttgt
     6421 agatgataat acattaactg tcaatgtaaa tagaatacgc tcaaaattaa atgaaattgg
     6481 tttagaagat tttatagaaa caaaaagagg attgggttat ataatcagtt aaaacaattc
     6541 taagtaggtg aatttatgag ctttaaagat tttctcaagg ataaactagg ttttttagta
     6601 tacaatttta ctttcttaat ttttacagta tcagttatta tattttctcc tgtagatgtt
     6661 tttctgactg atactatttt atatatatta gttgtaaatg ttgtgttttt atttttatat
     6721 ctatttatta gctacatcaa gaagaacaaa tttttagata agataaaaaa tgatacattc
     6781 aaaattaata tgaatgatat agaattagct agatgtaaaa atgaagaaaa aatatacttg
     6841 aacattatta aaaatcatca atatcaatgt aataacataa taaataataa tatcgagaaa
     6901 tttgaagaaa acaaagagat tttaaatatg tgggtacatg atgtaaagat acctatagct
     6961 ataataaaac tcattcttga acaaaatgaa aatttaattg atgaaaaaat ttcagatgac
     7021 atagatagtg aaatatttag aattgaaaac tccgttgaaa aaattttata tttatcaaga
     7081 ctcgaagatt ttcataagga ttttttaatt caagaggtaa atattgaaaa aatcattaga
     7141 aatattataa aaaaatactc aaaatatttt atttcaaaaa aaattatttt agacttaaat
     7201 aatttgaatt ttaaggtttt atctgatgaa aaatggctat acttcatatt cgagcaatta
     7261 ttgagtaatt ccctaaaata ctcctcatta ggagataata tatctatcta ttgcaaaaga
     7321 accaaaaatt atttacaatt aagagtggaa gatactggtt gtggtataaa aaaggaagat
     7381 ttaaatcgaa tattcaataa gggatttaca ggtaataatg gaagaaacaa ttctaaatct
     7441 acaggtcttg ggctttattt agtaaaagaa ctttgtgata agcttggtca taaaattgat
     7501 gtagattcag agtacaacca atatacaaaa tttactatta cattcaaatg tgatttatag
     7561 tatcttatta ttgatagttt attttcatta acttatattc aacttcttat agtaattcta
     7621 tatgtacatc ccagtatata attaagtagt ttttataaaa aggaggaaga catgtctttt
     7681 ctaaaaaaat ttaaaaataa aagatttaga aataacagtt ttaaaagtaa caactttaga
     7741 aatagcagtt ttggaagtag tggcttcaag tctaaaaata aaggttttaa aaacaatccc
     7801 tttaaaaata agagattcaa aatatatgca ggaatagcag taatcatact tttaattcta
     7861 ggagttcttg cttatgttga ttcgaaaaac actgaattac aagctaatga aaattttatc
     7921 gaaacatata ctattcctga aaatgaaaaa atatttataa atgggatggt tgttcctaag
     7981 caaactaagg acttcaatat atctggagat tatgagttaa gtgatgttaa tgttacaaat
     8041 ggtcaaaaag taaatcaagg agaccttctt tttactgcta aaaatccaac tataatagca
     8101 gaaatagata gtttaaaatc acaacttagt caatacaaaa aacaaaaaat atcactttct
     8161 gatattgctg aaaataaaga tgctattgct tctataaatg cacagataac tgctttaaac
     8221 tctcagatag cttctctgga taaaaaagct tatgacagag ttactgctcc ttttgatggc
     8281 acagtatact tgaatgacca aactggaaat ccagaccaac cagtttcatt tatgactatt
     8341 caaggtctag agttttatat gaaaggacaa gcaagtgaac aagatttacc taaaatgaag
     8401 attgaccaaa tggtcaatat actagttttt tcaactgacc aaaagcttac tggtagaata
     8461 agttttatat ctgataaacc atctactcca aatactgaaa tgggagctca gcagaataca
     8521 ctatcttatt atgatataaa catagcattt gataatcaag aaggattagt aaatggattc
     8581 catcttcaag catcacttga agtagctaat tcaagcttta aaataccagc atcttgtgta
     8641 cttaaagaca acaaacactc atatgtattt aaggatttag atggtatatt gaaaaagcaa
     8701 attgtagatg tagcaagtca aaatgatgat tttgctgtag ttagaggcgg tcttgaacaa
     8761 ggcgatataa taataaaaca ccctactaaa gaaatgaaag aaggagaccc agttcaaggc
     8821 gatggcgtaa ctgctggttc taataatgga gatactactc caaacaaaaa agtagaagaa
     8881 gttactatgg atgtgaatta atttacatat gttaattaaa ttagaaaata ttcaaaaata
     8941 ttacaaagta gggaaagatg aattacatgt tttaaaatcc ttaaatttag aaattgaatc
     9001 tggagaattt gtcatgataa tgggtaagtc tggtagtggt aaaactactt tacttaatat
     9061 cttaggtttt ctagatgtat ttgatgaagg aagatatata tttgatggaa cagatgtaac
     9121 taatttgagt gaaaatgaac gttctgtttt tagaaatatt aatattggat ttgttttcca
     9181 acaatttaat ttaatagaaa ctctaaatgt atatcaaaat gtagagttgc ctctaattta
     9241 caataaagca ctaaaaaaat ctaatagaga agaaattgtt aaagataaat taagttctgt
     9301 tgggcttttg gacaagctta agcagaaacc acttcaatta tctggtggtc aacaacagcg
     9361 tgtagccatt gcacgttgcc tagcaaatga ccctcagata atctttgctg acgaaccgac
     9421 tggggcatta gatagtgaaa ctagcagaga aattatggag cttttaacaa ggctcaacaa
     9481 acaaggtaag actataataa tggttacaca tgatcaagat ttaactaagt atgcaactaa
     9541 ggttatacga cttaaagatg gtgtgtttac ttcggaggtg taatcatgag tttattaaaa
     9601 aattctttgg ccaatttaaa aggtcacaag cttcgtgtat ttgtagcttt actctggatt
     9661 ataataggaa taacttcagt aatacttgtt agttctatag gtaatggatt tcaaaaagaa
     9721 attaagaaat ctgtaaacaa tgtaaaccct aataaaacta ctatttcgtt tgaatcagcc
     9781 gataacacag gtcttactga tgatatgagt atttttttaa aaccctttaa tgcaaaagat
     9841 ttagaagaat tatcatttgt tgaaggagta gaaagaatag ctccttcaag agatgggttt
     9901 aaccttgatt cagtttactc ttcacaagca tcttttgata aaaaaactac gtatgttgat
     9961 gttggaccag taaaaaaaga ttctaaaata aatttaattt gtggtagaga tttttcttta
    10021 gatgatgaaa aaagaaaagt aattttactt acattacaaa gtacaagtga aatttttgaa
    10081 aatccagaag atgccttagg tcatggtatc aacataaatg gaactatatt tgaaataata
    10141 ggggtattag atgattctca gcaaaaccaa gctggaggat tctttggagg ttaccaagat
    10201 atgcaattta ctacttccct agtacctaaa aaggcttttg acactttaat gagtcaaaat
    10261 tcttattcaa atgaaattta ccagttagac ttagtttctt ctaaaggcta taatgtaaat
    10321 gaagttgcaa acaatgttat agctaaatta tatgagatgc accctggtat caatggttct
    10381 tatactacac cagacccaac tgaacagaca gcttatcttg aaagcattaa ttccaatgta
    10441 aacaaatatg tatctataat aactgtagtt gcaatgtttg ttggtggtat tggtgtaatg
    10501 aatataatgt atgtatctgt tatggaaaga caaagagaaa ttggtataag acgtgctatt
    10561 ggagcaaaac ctaggtctat attatttcaa tttttagttg aagctgtttt tataacagta
    10621 tgtggtggaa ttttaggaac tatagttgga tttgctgcta ctaactatgt ttctaaatat
    10681 ataggttttg aagctatacc tagcttaaat agcttatttt atgcaattgt agctactata
    10741 ttgactggag ttgtatttgg tctgattcca gcctttaaag cttctaaact agaccctata
    10801 aaggcaattt ataagtagaa attgtataaa gattttcgat ttttagatta gtttatataa
    10861 aacatactta aatatataac gcactattat atatgataga ttaggaactt aatctttcca
    10921 aatataatag tgcgttcttt cataaaaagg attctcttcc ttactatatc taaattacca
    10981 agatgttatg ttctaattgt tcaaactaat acttccaatt ttgattaatc aatctcattc
    11041 tcctaatata caatctttta ctttcaatca ttgtacactc cgccttaaaa aaataatctt
    11101 aaattaggcg ttatatacaa tctccccatt acatattgta taattaacct ttccatataa
    11161 agatttacct ttaaatggtg aattatcagc tttagaaaca aagctttcaa ctgtccattc
    11221 ttcttctgga ttgaatataa caatgtctgc tacagcccct tcttctataa atccaatatt
    11281 taaattgtac aattttgcag gatttataga cattttttct attaactgca tcatactaag
    11341 atgattttta tgcaccaaat ttgttattcc aagagctaaa gaagtttcaa gcccaataat
    11401 tccacttgga gcttttataa actctctatc tttttcttct ttactatgag gtgcatggtc
    11461 tgttgctatt atttcaatag taccatcatt taatccctct ataatcttat ctctgtccca
    11521 tttacttctt agaggtggat tcatttttgc atttgttcca aactccaata catcttcctc
    11581 agtcaatgta aagtgatgtg gtgaagcttc tgcaactaca tttgcaccta agtattttgc
    11641 aaatctaacc atatcaacag atacacctga acttatatgt tgtatatcaa ctttagcacc
    11701 agtctttagt gccaacatac aatctctggc taccataaca tcttctgcta cacttgatgc
    11761 accttttaat ccaagttctg aagctacctt tcctgcattt acacctggat ttccaactaa
    11821 agaagggtct tcttcatgaa aactaagtgg tacatccaac tcttttgcct taatcattgc
    11881 ctccataatt agattgctat ccattaaagg taatccatca tcagtaaacc ctggtacacc
    11941 agctttttta agagcttcca tatcaactaa ctctttacct tcaaaacctt tagttatagc
    12001 tgctgcttgt aaaacattta tacatgcatt ttttgacttt tctttaacat aattaaaagt
    12061 atcttcatta tctacaatag gattagtatt tgccatacat ataacagttg taaatccacc
    12121 tctagcagca gatttagctc cactttctat atcttcttta tatgtaaacc cagggtctct
    12181 aaagtgtaca tgtacatcca ctaaacctgg agcaactaca ttaccactag catctattat
    12241 tttttcatat tcatcacttt catcaaactt gcctatttta taaattttat tttcttttat
    12301 aattaagtca atattctcat ctctttgact tttagggtct attactctac catttttaat
    12361 taaaatcata ttatttctcc tttatatttt taatttatat agactcatat aatattatac
    12421 attattttac atacttaaat acaaaattac gtttgacatc aatagccttt ttaggacaaa
    12481 gttcatggca acagtaacat cttatacatt tacttaagtc tataatagca cccttagtcc
    12541 ctatctctat agtggatgca ggacaagctt ccttgcattt tccacatttt atgcaatctt
    12601 gaaatcttac cacaggcttt ggtgttataa gctttgaaat aggttcattt aaaaattttg
    12661 gtagctttac ggaaaacaat cttaaatctt tacttacatt tggaatcttg taatttttta
    12721 taattaaatc ttttatatct tcacctaaaa tttcaatatc tgaaaaatct tcttgtatat
    12781 atcctctctt aatagcacct ctaattgtag caactttatt tggaagtaaa cttataatct
    12841 tacatgcaac aacatctata gcatatggat tttgtgatgc taataaaact cctatttctc
    12901 gagtagtacc agctgaaggt ccatcacctt ccatacctac tataccatcc ataattgtca
    12961 atgttggttt tatataatca caaatatcta aaagtacatt ttcgcaaaac aactcttctg
    13021 taggaaatct ataatgtatc tctgcttttt tcaatccagc tatgcatcca tataagtttt
    13081 ttgtaccacc agtaaaagtt gccattatat gagtctttaa tttacaaaga tttattatat
    13141 aatctgaatc tactattggt ttaataatat ccatatattt tagaacatgt gctttttcac
    13201 tatgtatctt aacatctgaa acatcataat ttaacttaat gcccagttca tttgctactt
    13261 cttcaattcc agatgcctta taaatgctct taagagaact ttgattatat ggaccaccag
    13321 ggctatctgc tattgtaata tcacaattta actctaaaag tttttcacat acaactttta
    13381 caaccatagg atgtgtagtt gtagcttctt caggcttttt tttcattaat aaatttggtt
    13441 ttataagtac tttgcttcct tttttaacaa acttatctat accaccaata tcctctaaat
    13501 ttttttcaat agataacttt acttcttcat aattgtaagt tttacaactc ctaacagata
    13561 ccgacttcat attatctcca tccttctatc acaacagttt atttatataa tttaaaaaag
    13621 gagaaaccaa agtttctcct tttaatctat gttttaattg ttttaattat tattttccaa
    13681 aatgtctgtt taataatcct aagaatgctt taccatgtct agcttcgtct ttacacattt
    13741 catgtactgt atcatgtata gcatctaatc ctaattcttt agctctctta gctaatttta
    13801 attttccatc agttgcacca tactcagcat caactctaac tcttaagttt tcttttgtat
    13861 ctgcaactac aacttctcca agaagttctg caaatttagc agcatgttca gcctcttcaa
    13921 aagctattct cttatacgct tcagctactt ctggataacc ttctctatca gcttgtctac
    13981 tcattgctaa atacattcct acttctgtac actcaccagt aaagttagct cttaatcctt
    14041 cgattatttc tgcatctaca ccttgagcta ctcctattct atgttcatca gcccaaacca
    14101 tttcgccttt catttcttca aatttatcag ctccaacttt acatactgga cattgtgctg
    14161 gtgcagcatc tccttcatgt atatatccac atactgtaca aacaaatttt ttcataatta
    14221 atttcctccc aattttatat aattaaattt ttatttttaa gtttaataac ttgctacaag
    14281 aatatatata ccccagactt tatagcttaa acactaaaaa taaaatttat ttcctaatta
    14341 attttatcac tttgaaacac cagtatcaac caagttttga tgtcaaattt ctacataata
    14401 cataatttat ttgattaatt aggaaatagt tgtcatatta aatactttct aatgaagact
    14461 cctctgaatc tttaagtttc acttttttgc tctttaaaaa agataattta gtttctgcag
    14521 ttatgattgc aacaaatatc aagatactac caataaccaa ttttgctgta ataagctcct
    14581 ttaaaattat tatagaaaat attgtaccaa atacagcctc tgtggataat attatagctg
    14641 ttctagttcc atcaactctt ttttgacata tagtctgaaa taaaaatcca attgtagtac
    14701 taaatacagc tagatacata gttcccatgt aaccagataa ttcagcttcc atctttaatt
    14761 gacctgcaaa tgtttgaact acaacagaca ttaaaaacgc tactgtaaat tgaatagctg
    14821 tcaatgccat tgggttgtta tcttttgcaa actcacttgt aaagaatata tgaaatgcaa
    14881 atccgaatga acatataagt gttagaaaat ctccaaaatt aatagagaaa tcagcttcta
    14941 aagaaagtat cccaattcct ataagagcta ccaaactact tattattcct attttatcaa
    15001 gttttcttct ataaagtata aatcctatga atggaactat tacaacatta gctgctgtta
    15061 taaacgcatt tttagatggt gtagtgtaca taagtccaat agtttgtact gtgaatgcta
    15121 gaaataaaaa tattcctaaa atccctcctg cttttaataa ttttttaccc atattggcac
    15181 gaatctgttt aaaaaacagt aaattcataa ttatagctgc caagaaaaat cttaaagtta
    15241 tgatttgtag aggtgtaagc ccaccgtcta atgcaaattg ggttgctaca aagccacttc
    15301 cccatataat tgctataaat attagtccta tttctccaaa gtattttttc aataaagctc
    15361 cctccttttt atttttttgc aaaattataa accccctaaa cagcaagaga gtttttctat
    15421 aagcattact ataaattaat atagtaaatt ttttcagtat tttaaatcta aatttacata
    15481 cattttgagc tcaaatttat atc
//
LOCUS       DAMKMI010000097        15417 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMKMI010000097 DAMKMI010000000
VERSION     DAMKMI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15417
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.100"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(197..529)
                     /locus_tag="PC429_002636"
     CDS             complement(197..529)
                     /locus_tag="PC429_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF3848134.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(773..1414)
                     /locus_tag="PC429_002637"
     CDS             complement(773..1414)
                     /locus_tag="PC429_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF3848135.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFVYSYLMYKISGKNN"
     gene            complement(1395..1688)
                     /locus_tag="PC429_002638"
     CDS             complement(1395..1688)
                     /locus_tag="PC429_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3848136.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2081..2131
                     /locus_tag="PC429_002639"
     CDS             2081..2131
                     /locus_tag="PC429_002639"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848137.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(2657..3409)
                     /locus_tag="PC429_002640"
     CDS             complement(2657..3409)
                     /locus_tag="PC429_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF3848138.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3468..5123)
                     /locus_tag="PC429_002641"
     CDS             complement(3468..5123)
                     /locus_tag="PC429_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3848139.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSVSDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6395..7474)
                     /locus_tag="PC429_002642"
     CDS             complement(6395..7474)
                     /locus_tag="PC429_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3848140.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7558..8214)
                     /locus_tag="PC429_002643"
     CDS             complement(7558..8214)
                     /locus_tag="PC429_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3848141.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8536..8742
                     /locus_tag="PC429_002644"
     CDS             8536..8742
                     /locus_tag="PC429_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848142.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8895..9323)
                     /locus_tag="PC429_002645"
     CDS             complement(8895..9323)
                     /locus_tag="PC429_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF3848143.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9799..10737)
                     /locus_tag="PC429_002646"
     CDS             complement(9799..10737)
                     /locus_tag="PC429_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF3848144.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
     gene            complement(11251..12063)
                     /locus_tag="PC429_002647"
     CDS             complement(11251..12063)
                     /locus_tag="PC429_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3848145.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
     gene            complement(12086..12718)
                     /locus_tag="PC429_002648"
     CDS             complement(12086..12718)
                     /locus_tag="PC429_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3848146.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            complement(13062..14717)
                     /gene="ilvD"
                     /locus_tag="PC429_002649"
     CDS             complement(13062..14717)
                     /gene="ilvD"
                     /locus_tag="PC429_002649"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF3848147.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLNFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            complement(15041..15340)
                     /locus_tag="PC429_002650"
     CDS             complement(15041..15340)
                     /locus_tag="PC429_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848148.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT         5079 a         2740 c         1644 g         5954 t
ORIGIN      
        1 atttaataat gacaatatac tattctcaat acaatctatg atgtaaataa tttaaaacta
       61 ctatattatt taaaaattta tatttatata tgcaattcta aaaacttaat aagaagtgaa
      121 cttagaaaat aaagcttaat ttcaaaggct taaatagact attattatta gtctaaataa
      181 actacgtgta tcaaaattat atattaaatt cttctaaaat ttttattgca aatcttgcgt
      241 ttggatatgt aatagaccct ccaccaatta cacctatttt tatagcttct ataatttcat
      301 ctttacttgc tccagaatta atacaaccct caatatgata acaaatacac tcatcacatc
      361 ggcttaaaat tgaaatagct agtcccatta attccttatt ttttttactt atagcccctt
      421 ctgaataagt cttatcatca aggcttccaa atactctaaa aaactcatct gtttcactta
      481 attttttatt gtaaaatccc ctatattctt tcatttctct agtattcatc ttctacctcc
      541 tatttttttc ttataagagt atcacactgt acttatattt tcaatattaa atacaaattt
      601 tcattttatg ttcattagcc taatttatat tattttttga aatctcaata agttatcata
      661 taagtaagat tttttcagaa ctaaaataga aatctcagta atttctttat tatctatctc
      721 agttctgaaa tatattattc ttatccaaac attattatag tgattaatta tattagttat
      781 ttttacctga aattttatac atcaaataag aatatacaaa tggtagtatt acaattacaa
      841 agattacaaa gatattataa aaaggaatat taataaatgc attaataatt aataatattc
      901 caccaataag ccaaatccat ccagctaaat ggtgtgtctt attccaatta tcttcatcat
      961 taagagtcca tggtaaaatt attcctactg agtaatttcg tttacattta ggaagatagt
     1021 ttccaaataa aataactatt attcctaaaa ttaatcctgt ataaaaatta gactccaaac
     1081 taactcctgt tgaatataaa accatcatta tttgtataat tactgaaagt atagcaactc
     1141 cccattttga taaattttgt ataacatttg aaaaattagc tttcttaggc tcattctcaa
     1201 gtctaaacca tgtatacaaa tggatacata gcattaacaa tggtacacca aaagctgcta
     1261 taacctttgg agcataatta tctggatttc cataattatc aaaatgaatt gcaacttcat
     1321 ttggtaatct attataaaaa tatattgata caataaatgg tataaaacat attaaagtag
     1381 ataaaataaa ttgtttattt atcgcttttt tcattatctt tttctccctt ctccataaat
     1441 tgcgaaaacc aaagcataac ctcttcgaaa actgaaatat ttatttcgta gaaaataaag
     1501 ttcttatact ttgtttcaaa tatgagtcct gcctttttta gttgagatag atgataagaa
     1561 attgtagcat tcgtcatatt aaatttttca ctaatttctc ctgctgacat tttattctcc
     1621 ctcaacataa ccaatatttc tcttcttata gggtctgaaa gagctttgaa tgtttctgga
     1681 aaccccaaca ccatcacctc gctatttaga atttcatcta aatagaatat aacataacat
     1741 ccattttatt tcaataacta tttagaattt tttctaaata gttatttcta aatatttatt
     1801 gaaaattttc atattaagat tctcaattta cttaagtcgc ttttatttaa tcttacttta
     1861 tctaattttt ttaacttaac aaaagtgtat tgcaccataa ttgttaaagc aaaaacctaa
     1921 tgactgaggt acttatatat ataacaatac ttttaatttt aaattaaaag tagctaaacc
     1981 caatttaaaa agtagttaag tcctatttaa atagaaaaga atgaaacgag aaatatactg
     2041 tataaaagta cataaaaaca tctactaatt agatacaact ataacagaat atatttatta
     2101 ttataataac aaaagaataa aaaaaaatta atttagcata gccttgttaa atatagattt
     2161 gaatatgaat ttaaagttat gtaaaattaa aaatacagga atgaaaattc cattcctgta
     2221 taaaatctaa cttttgagtc tcagtacaat cttattttga gacaatcttt tttgttttat
     2281 attttcttat atttaaaata taacgcatat cttatgcaac atattcatat ttttctttat
     2341 attctttcat cgcatcattc caagcgtctt gtactgttac acctctttta ttagcaattt
     2401 tttgtgccag ttcaattatc ttcatagctc ttcttgatat gttcataaac attcctccta
     2461 caaattacca tatatttcat atatataaat caaggttata tttttatgat atataatcct
     2521 tctattgtaa gattacagtt tatatgtgta ttatatatga actatattag aagataatta
     2581 gaatttttat aaagctatat gattagatta aatataaaag ctttacaatt acttataaat
     2641 ttaaaaagtc tataaactaa actaatgttt gatataccaa ttttacgacc ataaataatg
     2701 ccattactaa aaatattggt tttatcatct ttgaaccttt ctttatagca actttagcac
     2761 ctacataagc tcccattatc atcactaatg caaacactat agcaatttta taatctactt
     2821 gaccatttat catgaataat aacaatgaag ttatattact tgttaagttt aatatttttg
     2881 tatttgcaga agcatgtaaa aaatcatatc catatatttt tataaatcca aaagtcaaaa
     2941 aagttcctgt acctggacca aaaaaaccat cataaaaacc cattataaga gccataagca
     3001 tccctagtct taaatttttc ttattaacag cttcaaaatt atcttcttga cctaaatttt
     3061 tagaaataaa ggtgtaaata gccactatta aaatcatagc cacaaccaaa acatttaata
     3121 tctcttcact tattgataat acacatctca cacctagaac actaccaata atagtaaatg
     3181 gtactaattt ttttaaaagg ttgttattaa tttttccaga ctttgcatat ctataggcac
     3241 tacttataca tcctgctgat gctgcaaatt tatttgtacc aatggcaaga tgtactggca
     3301 ctccaattgc cataagaaca ggcatgctta taagtcctcc tcctcctgct atagaatcta
     3361 caaaagcagc taaaaacccc cctatacata aaaaaataac tgaaaacata aaattcctcc
     3421 atcattctct ttcaattttt cttacaatat atattatata atcttattta atacttatta
     3481 tccagcatat tctttctata ttcatttgga gttatactat taaacttctt aaatatttgt
     3541 gaaaaataac tactgttatt aaaacctact ttcttacaaa tatcactaac actcatgctt
     3601 ctatctaaca gatatttttt agattcttct atcctaagaa gatttatata ctcagttatt
     3661 gaaactttat ttctttcatt aaaaatagaa ctcaaataat ttggacttat aaaaaaaaca
     3721 tctgatatac tattcaaatt tatattttct ttataatatt tttttatata ttcgtttata
     3781 ttttcaatta gatttccttt atcttttctc caaaatgctc tattcttatc aatacactct
     3841 ttggaaaatt caaataatac attccataaa tcttcatcac tgtttacaag tgatattttt
     3901 ttattatatt tatttcttaa ataagatata agttttaaat cctctaaatc tctaagcact
     3961 gttctagaca atacaaatat catttcctga atagtatctc taacatcatt atatcttaat
     4021 ttgtttgtat ctattagatt ataaacacct tctaaattca tatttttaat ttctttttca
     4081 atataaagtt ctaattcaaa gggatattct tttgaagaat tattgaacat atctatcgga
     4141 ttaataatat actcttcttc taataatcta ctatttgctt ttttaagtgc ctcttcaagt
     4201 cttatatttt cttttctact cttcaatatc tcctcatctt ttatactaag ttgattttca
     4261 aaaaaattcc tcttagctat ttcctgaata aattcagaca tcattaataa attttctttt
     4321 aatacatata atctactttt aggatttaat ggaaattcat tgtaaatttt gtttatgtat
     4381 tttatatcaa taccagaact acttgataat tcagataaat ttttatctat ataaatagac
     4441 ttatatacta taaaattacc acattctata tatccaacta tattttcttc taatactata
     4501 ggtatctcta aactggaaac accatactca caatgataca ctttttcaaa gttatcatta
     4561 tttatgcttt taacaagttt tttagtacac ggttttatat ttttacaaat atcgcaaaat
     4621 ttattttctt tcttagtctc aaatatttct ttatacttta agtcatatac agatattcta
     4681 agcttcaata tattagatat ttgatttgca agattttcaa ttatgtttac attaactaac
     4741 tcctgtatat tgttttcaga aaatactata ggtatttgtc tcaaactcga aaatttatga
     4801 ggaacataag taattaatat tactaaatca attttaccag tgtttataat ttcatgttct
     4861 gaatatggtg ggcaataaac tatagatgtt tcctttatat tgaccttttt accatcaatt
     4921 atttgtattc cttctccctg aatcacatat aaaagctgtt cttcacttaa atgaaaatgc
     4981 ttttcttggc agtttcccgg aaaaaatctt acaattccag cactaagtct attgaattgt
     5041 gtttttgatg gttcgtgtat ccacataacc tctccccaat caaatttttg tacttcatta
     5101 tttgactcaa taaaatccac cataaactca cctcgtagtt tttgtctaaa tattttttct
     5161 tcccttctta tattaaaata atatcatcat cattaacttt cattactata aaatcatcat
     5221 actcttttaa tactcctgta attttagttg cacttagttt actacaattc agttttttaa
     5281 tatattgata aattctataa ttactttttt attttgtaaa atcagtgttt tcccaaaata
     5341 attatatcta taaatgaaag tatttttact gcttacatca atatatgtta tcataatttt
     5401 cctttttatt gcatatataa ataactgtat ataacattgt ccaatactat atagaaatac
     5461 atctaagaaa gaatattatt aaattgttcc aattaatatt atttttctta tagaaataaa
     5521 aaactgttgg attcttttag aaaaattatt ttttacatac cttttttgct taaatagtct
     5581 atcttctcag ctattttgta tatacactct tctagttcat gattttattt taatatcttg
     5641 cttatattgc tgaaataatt aaattctttt atctatcata ttgtctctta tatcacaaac
     5701 atattgtttc ttatatcaca aaaatgaaag cattcttata tcttcaatat ataaacctgc
     5761 tttttttaat cttgtaataa atataggata ctctatattc tcttcctaga aataactaat
     5821 accacttatt tttctatcat atagaaagta ataaatcttt ttcttaataa aattcaatag
     5881 taagtggaat aaaattttat atgaataaga acattataaa aatactttat acaatagtca
     5941 ataaaagata ttagcttcaa attgtgttga agttgtatta tttaaagtgg aaaagcaaat
     6001 aaaaaagaaa taatggtata agtagaaaaa tatacttaaa acaaggcgaa gcctctcttg
     6061 tatctaaatc aagtagagaa ttaaatctaa aaaagaaaat aaatataaaa gatgactcct
     6121 gtacattaca agaatcatct tatggtttca taatcactcc atggttttta actgtctgat
     6181 ataaaggtct cagttcactc atagcatgac caccttttcc gattgctgac cccgtcactt
     6241 atggacaaaa tgtccataag tgatagtcct ctcttaaaac cctctaaata gatatatctc
     6301 aagcacctca ttgaaaccag ttgctggaaa caaactgaag cgttctggaa tagtaaaaga
     6361 aattgccacc atatccacgt tactgtccta gttgttactt tgctaacttc ggagcgattt
     6421 cctctaacaa tacgcagtca aattctgcct ttttgctaag aaatggcaga tggtctgcat
     6481 ctggtataaa aaaaatctgt ttgtctggcg ccactatttc atcaaaaaat ttcttcgctg
     6541 gaagataggg gctattatag tcattttctc ccatgatgta gtatacaggg acctgatagt
     6601 caactccata atttcttatg tcagcttctt ccactaaaaa ccgcaataca tcaccttgat
     6661 tgtcaatcgc atttggattg acaatggccg tattttttaa ctctctcaat gaataatatg
     6721 gggttgccag tatactgatt atattgccca aatccatatt tatagaaagc ccatacttgc
     6781 tttggtatgt tcttactgtc ataagagctg ccaaatagct tggaccatat tttgctggcg
     6841 gatatggtgc aatagcttcc agcgccttga tatctttttg cttccctgca gctttcacca
     6901 tttcaagtgt cttttcatag cccacgcgct cattgtccac catattaaca gtctgcgcaa
     6961 tcccaatgta cgcgctgtaa tcctccggat aggtttgcac agccatagtt cccagcactg
     7021 ttccccagct atgacctata aggataattt tctccttgtt gaactcttgc ttcaaatatg
     7081 ctgtcacctc atggacgtct tgcagaacac gctccgcgct catggtctgc aaaactgctg
     7141 ccggatcgtt cgcaaagtat gttttgccta cgtttcgctg atcccagttc accacagtaa
     7201 aatcattttc ccactcatac tggtacagat gtaaaaatgg catcattgaa ttaccaggac
     7261 ccccatgaac ccaaaggatg atggggttgt ctgcgttttg tccccggata tataacgcct
     7321 gctgtatgcc gccgatctcc accatttcca tgcggtcgat tccaggagct tctattttat
     7381 ggcttgtacg tataatcgtg ggggctatca gacgcaaacc ggcaaacgcc agaacaaaaa
     7441 cagcaactag tatgcaaagt gtgattaata tcattttaag taccttcttt catgaatttt
     7501 tttagccata gatatctcct ctaacattaa aaaaagtatg ttgctatttt agttttgtta
     7561 ctctacctgt cccattggtt tcctttcata aaaaatatta attgattcta taatctcatc
     7621 ttccaggata tcaactccta tttgtctcat aagaaactgt aagaattgaa accgtttacg
     7681 aagtgtagga aagtccccct gaaatacatc tgtatcacac caaaagttaa agagcagcaa
     7741 aattacctct gcgcattcat ctgggaactc tgtgatgatg cttctatcct ctatgccttc
     7801 tcgtaaaaca tttgctaata ttggtgctag ttttttcaaa ttatcctgtg taaatgccag
     7861 tatgatatgc ggactttcta tcgcgcttgt aatcatgtta ctagattctt ttatcacctc
     7921 atccgttaaa tgccgcctaa ctaaacttct aagcttctct tttgctgtaa gtccatgcat
     7981 ttcaccgagc cattgattta ctgtctctat tatctgttca aagcgccttt ccatgaccac
     8041 attaaaaata tcttcttttg aactgaagtg atgaaaaatt ccgccctttg acattcccga
     8101 agcatcaaca atattttgca tacttgtttt gtcgtatcct ttttcagcaa acaactttgc
     8161 agaaatggta atgatatttt caatagcttg ctttggattg ttctttctag tcattatata
     8221 actccttctt attaaaaacc gactgttggt ttttattata taagctattc ctgcttctgt
     8281 caagtaacat tgtgtttatg gcaaaacaat aaggtttggt ggttagacag gtattaatta
     8341 gactaatgcg actttcccct agatggaacc cttggtaaat ttgcaatctt taccggcttt
     8401 gattatatct gtaagcaagg caggtatggc cacgcctgcc ctgtttgacc ctcggatatt
     8461 cattcctatc cctcctctcc ttagccatta caccaaaacg ccgctcattg tctccaatgg
     8521 cggcgtttta aagatatgat ttctcatgct gctgaaatat gcaaaaaata taagatgccc
     8581 tttattattt ttcctacaat agaaaagaaa agtattgaag aatgggttaa tattggagca
     8641 aatatgcttt gttttgcaca agatactacc tttatcactt atgttatcaa tcaattatta
     8701 tctttttata atgagattgt accaattcaa aaacaacatt aaattataaa tctaatttta
     8761 tattaaaaga gactaatatt ttatctatct ctattaagga tattttctta tgtatcataa
     8821 tattagtctc tcttattatt atatactgct atcatcatta gttgttattt atactatttt
     8881 tataatatac aaatttattc agctaacgtt ttgcctaatt ctatacactc atcttttgct
     8941 tcttcatttg ggtcttccat tgctgtaatt ccttctccac caataattgt agcaccagca
     9001 ttttgcatac gttcttccca gtctctcatc cattcacaat ttccccatcc atatgaacca
     9061 aataatccaa tctgtttacc tgataccata gattctaatt ctgaaacaaa tggctccata
     9121 tacccctctt ctagttgctc tgctcccata gatgggcaac ctaatgcaaa tttatcttct
     9181 tcttttaaat cacttggttt cagtaagctc acatctaaaa cttctggtgt cttacctttt
     9241 aacttcacac cttcagccac aaaatttgcc attttttcag tatttcctgt tccactccaa
     9301 taaacaatat atattttact catttttacc tcctgtagta ttttttaatt aatcaatagt
     9361 tagttatagc taactaacat ctaaaaaaca aaagatttct ttatctgaaa tctctttatt
     9421 tttctaaata tagaatatca tagttgatat tgaaattcaa tacttattga taatatttat
     9481 catttatatt ttatattcat tttataatat caaaacactg tatcgtattt ctaatctttc
     9541 atagaaaaat ttctaactta tagtactttt gcatctctta taaatctttt atatgtataa
     9601 gtacatagaa aaacacacct tttccagatg tgctatctat aatatcgtat atttattttt
     9661 caattctaac tagctaatat gtttataaat tttatcactt aataccttta tacattgatg
     9721 taaaatctta cacctaagta atttttatta tttgtctcga ctctatataa tatagctatt
     9781 ttatcatcaa atcaaaaatt atcttttact attattcttg tatttataga gatttaaata
     9841 taagtatcca tttccttgtt ccgttctagg tattccaagc gctctcgtac attttattaa
     9901 ttgtgcatac agttcagctt catctaaatc tctttcaaac tcctctcttg cccattcttt
     9961 aattagtgct aatgagcctg atacgtatgg tgcactcata ctagtaccac tcataatagc
    10021 aagtttatta tccatataag ttgatataat atttcttcct ggagctacca aatctattgt
    10081 agtatttgaa ttactaaact tttcaaccaa atagttctca tttattgcac ctacttctat
    10141 tacctcagca taactggctg gataactata ctcacttgta ctagaatcac catctccatt
    10201 attacctgct gcacacacta cagaaatatt attttttact gcttgcatga cagcattttt
    10261 taaattctta tcatctttgt ttcccccaag agacatagat ataatatcaa ccttgttatt
    10321 tacagcaaag ttaatagcat taattatact ttgatatgtt ccggtaccat ctttatttaa
    10381 tgcttttgct attaataatt tacaatctgg agctactccc ataacttcat tattataatt
    10441 agatgcagct ataatacccg ccacatgagt tccatgacca ttaaaatcct catatatatt
    10501 tttatttcca ttactgtcat cactaaaatt tgcaccacca attatttttc cttttaaaag
    10561 aggatgagat atatcacaac ctgtatctat tataccaact acaatatttt taccagtata
    10621 accttcatcc cacattcccc tagcattcat ttgttttatt ccatatggga atttattttt
    10681 tgcacctctt agattttcat ttatttcata tggaattagt ttcattcttt tattcatcat
    10741 atatcctcct aaaaatttta ttgcttataa aaatcatgaa ttttatttta taataggttt
    10801 ataaatcaaa ttaaaaccta tatctactta tttatattta tgatatattt tttattttta
    10861 ggagtcatat tttattgtat ataaaataat atttagataa ctatattaaa aaatacatgt
    10921 ttgcttatat acaaaactta ctaaaatatc ttcaatattt ttgtatacaa acattatatt
    10981 ttttatataa aaatctcata aaaattttga ttatattttc ataagttaac aaatgtgggt
    11041 ataatatgaa tttacaaaat tcctctgttt gtataagtgc tttaccaata cactaatata
    11101 taatcacatt gaagctatat ttgtatttat agtagataat gtatcaaaaa ttggttatga
    11161 atagtaaaaa taccagttaa gcatattttc atgtttctat ttttaacatt aaatttccaa
    11221 tcttcatatg tatattttta acaaaggtgt ttacttattt aaaaactcta aaactctttc
    11281 attaaaatcc tgtgcttctt catatgtagc gtgtcccaat ccctcataga taaatatttc
    11341 gcttttttta atcttttctg ctaaatgaaa agaagcatta tttccaacaa ttttatcatt
    11401 agcacctcct ataattaagg ttggacatgt tattttattt aattcggaaa aggcattatg
    11461 ttctatgcaa gaggtcgctt gaataataaa tcgtttaaaa tcctttggtt tacctacttt
    11521 tcctaaaaaa ggaataaata attggtattt ttttaaatat ctttcagaat aggatttctt
    11581 tgctgtgtca atcattaaat cattgtaatt ctgcttcttc gccatatcaa tccaactaca
    11641 aataacattt tggatagtat cattttgctt tgaagaagta actgccaaca caagtttctc
    11701 aactaactca ggataatcaa ttgccagata ttgagctatc attccacctt gagaaacacc
    11761 cattacttct gctttcataa ttccaagttt tttcattata tctgcttggt ctttagccat
    11821 atcccttgtg gaatattttt ctgtaatttg attttttcta ctaaatacat aaaccttata
    11881 atcttttgca aattgcttat atctaaaagc aaaagcaatc gcctgtattt ttccatgtaa
    11941 aggaaataaa ccatctccta atcctggtaa aataattagt gtcttcttac cactaccaaa
    12001 ttcaatataa ttcactgtat tacttcctat catcatttga caactttttg cattataaaa
    12061 catagtctat tcctcgcttt tcctattata attgtctaaa taaatcttta aatttttgaa
    12121 taccatactc atcaattaga ggaatatcca ttttttttaa catgaataat aaaaaatcca
    12181 acctattaca tacttcactg tattcacgtt taaataatac agggtcaagt aaaatatcaa
    12241 tactaaaaac gagcatttca gctaattcct ctggatattc acaagaaaat tctttatttt
    12301 caacaccttg tttaattatc ttagcaatat tgggtgcaac tacattgttg agattacgaa
    12361 cttcctctat taaagcgaat gggatttttt ctaaccagct acaatgtacc atttttctac
    12421 gtgtgtaatc attcgcacta tgaacaacca attctattat tttttcattt gctgtaaggg
    12481 tgtcatcagc aacaagatgc tctaaaaaat tattcatttg tgagtaaagt tcttttgtga
    12541 cacttctcag tatatcttct ttagttttaa agtgatgata cattgcacct ctagatagac
    12601 cagataaatt gataatatct tgtgtagatg ttgaatcata tcctttagac gaaaatagtg
    12661 aaaatgctga ttggataatt ttttctttac tatttttact tttttcttct ctattcatat
    12721 ttccctccta aaaacagact gtctgtttgt tttttattat acattttaat atttattatg
    12781 tcaagaatta actgactttc aatttcttca tagttcagat ggaacgaaaa gcattccaca
    12841 tcaaactttt aaaaagttgt attacctcaa caaggtctaa aagatacatc tctatgctgt
    12901 actcgtattc cttataatag agatgtattg ttttcaaaga taatcaagat tttgctgtgt
    12961 ttttcataca aaataaaaag gatacctcat tagtgctata acacctaaga tatccaatta
    13021 atattttctt aactttttct aatttacata aatatagtct actattttaa aactgctcct
    13081 gtatttgctg atgttaccag tttagcatat ctacttaagt atccatgctt tattttcggt
    13141 tctaaaggtt taaattttaa ttttctattt tctatttcaa cttcatctac ttttaactct
    13201 aattttttgt ctggtatgtt tatagaaatt atatctcctt cttcaactaa cccgattaaa
    13261 ccaccttcca tagcttctgg agaaatatgg cctatagatg ctcctctagt tgctcctgaa
    13321 aaacgaccat cagtaagaag tgctacatgc ttatcaagtc ccattcctgc aactgctgat
    13381 gtaggagata gcatttcttt cattcctgga ccaccctttg gaccttcata tcttataact
    13441 ataacatcac ctttgtttat ttttttacca aaaatagcat ttacagcttc ttcttctgaa
    13501 ttaaaaactc ttgctggtcc ttcatgaact aacatttctt ctgcaactgc tgactttttt
    13561 acaacagctc catttagagc aagatttcct ttcaatattg caagcccacc ttggttacta
    13621 tatggattct ttaatgtatg tattacttct tcattttcta tttcacaatt tcttatattt
    13681 tcccctatag tcttgcctgt tacggttttg caatctaaat ttattccatt tatctttgaa
    13741 agttcgttca ttatagctgg tattcctcct gccatatgta aatcttcaat atgatgtttt
    13801 ccacttggac ttaattttgt taaacaagga gttttttcac ttatttcatc aaaaaaattt
    13861 aagttaagct ctattcctga ttcataagct attgcaggta agtgaagtac tgtatttgta
    13921 gaaccagcca ttgccatatc cacagctata gcatttttaa aagcatctat tgttaaaata
    13981 tctctaggtt ttatgtcgtt cttaagtaac tccataacat acatacctgc atattttgct
    14041 atcctttttc tttctccaga atgtgacgca gcagtaccat tatatggtat acccatgcct
    14101 aaagcttctg ttaaacaatt catgctattt gctgtgaaaa gaccagaaca tgaaccacat
    14161 ccaggacaag agttcatggc taacgcttct aattcttctt cacttactgt tccatcttta
    14221 catgctccaa caccctccat tgcagagtta aaatcatata cttttccatt ttttttacct
    14281 ggaagcatag gccctccact cacaacaata cttggtatat taagtcttgc agcagccatt
    14341 aacatacctg gcactatctt gtcacaatta ggtataagta ccaaagcatc aaaaccatgt
    14401 gccatagcca tagcttctat tgaatctgct attaattctc ttgaagccag ggaataattc
    14461 attccaacat gccccatagc tataccatca cataccccta tagcaggaaa ttctaatggc
    14521 aaaccaccac tcattcttac tccatttttt gcagcttctg ctatttcatc aagatgcaga
    14581 tgaccaggaa tcacctcatt ttgtgcattt actataccta ttaatggtct ttcaatttct
    14641 tctttagtaa gacccattcc atacattaac gctctctttg gagctccttc tattcctttt
    14701 tttatatcac ttctcataat attacctctt tcttactttt ttaaattttc ataatattat
    14761 tataatatat ttttctatta attaattacg aaaattgtaa aaaaatatta gcatatacta
    14821 atgaaaattt attacgattt tttatcaata tcttacacaa ttgtattata taatattatc
    14881 ttttcaatat attttataaa ctaatatatt ttacaaattg ttgagtaata ttttatgata
    14941 taaatcagaa gaggcacttc tataatagaa agtgctcaac tatgaataag attaataata
    15001 tatttgcctt agatatatag acttttataa atattaaaga ttaatcaaat ctgatgtttg
    15061 aaactttatc ccaattaatc ttccttgaat agtcttctgg atgtgataat ctaactatat
    15121 gagttgaatt atcatagctg taataaattg tagtagtacc atcaactgga ttctttttgt
    15181 atccttgata cacagcacta tttggtactt tcttttcaac aaagctatat gcagatgaag
    15241 tatcaatagt ttctatttta ttaggaaata taaacatata aaaagctact actataaaaa
    15301 tacatatgca tgtaatacta taccatttaa tccatttcat aaaattcctc ctactttacc
    15361 aaaatatact attattattt ccatatattt ataatattta tgcatacgat aaaattt
//
LOCUS       DAMKMI010000098        15181 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMKMI010000098 DAMKMI010000000
VERSION     DAMKMI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.102"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            46..1476
                     /locus_tag="PC429_002651"
     CDS             46..1476
                     /locus_tag="PC429_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3848149.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
     gene            1676..2260
                     /locus_tag="PC429_002652"
     CDS             1676..2260
                     /locus_tag="PC429_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF3848150.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(2364..3143)
                     /locus_tag="PC429_002653"
     CDS             complement(2364..3143)
                     /locus_tag="PC429_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3848151.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(3201..4457)
                     /locus_tag="PC429_002654"
     CDS             complement(3201..4457)
                     /locus_tag="PC429_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF3848152.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            4715..5614
                     /locus_tag="PC429_002655"
     CDS             4715..5614
                     /locus_tag="PC429_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF3848153.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            5638..7086
                     /gene="ddlR"
                     /locus_tag="PC429_002656"
     CDS             5638..7086
                     /gene="ddlR"
                     /locus_tag="PC429_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF3848154.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            7145..7951
                     /locus_tag="PC429_002657"
     CDS             7145..7951
                     /locus_tag="PC429_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3848155.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            7977..9896
                     /locus_tag="PC429_002658"
     CDS             7977..9896
                     /locus_tag="PC429_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3848156.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(10138..10485)
                     /locus_tag="PC429_002659"
     CDS             complement(10138..10485)
                     /locus_tag="PC429_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF3848157.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(10472..12400)
                     /locus_tag="PC429_002660"
     CDS             complement(10472..12400)
                     /locus_tag="PC429_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF3848158.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            complement(12811..15000)
                     /locus_tag="PC429_002661"
     CDS             complement(12811..15000)
                     /locus_tag="PC429_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF3848159.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
BASE COUNT         5617 a         2286 c         1967 g         5311 t
ORIGIN      
        1 acctatttta atatatcatt aaaaatttag gaggaaaata taaaaatgaa tttagatttg
       61 gaattttatc aaaaaatatt agaagcttcc catgatgaaa tatgtgtttc tgatgataaa
      121 ggaataataa tatattgtaa taaagcattt gaagaaaatt atggtctaaa aaaagaagat
      181 attttaggaa aaaatgtttc ttttttagaa gactctggat attcaacaaa aagccccata
      241 ccaattgtat taaaaacaaa gactaaattt tcactggaac aagatactca aacagggaaa
      301 aagcttatca taactgctac ccctatattt gatgaaaatg gaaacttaga atttactgtt
      361 gaaaattgta gagatataac tgaacttaat aatataaaaa ataaacttga agacacaaaa
      421 aagcaagtaa aaaaatataa atctgaagta gaaacactat acagaactgc acttagaatt
      481 gaggatactg taattatgga cggaatagtt atgagaccaa tcataaacac agtaaatcat
      541 gtttctaaaa cggatgtaag tgtactttta ttgggtgaat ctggaacagg aaaaagctca
      601 cttgctagat atatacacca caacagcaat agatctaatg gcccatttat aacaataaat
      661 tgtgcaacta tatctccaca acttcttgaa tctgaacttt ttggatatac ttcaggtgct
      721 tttacaggtg caagtactaa aggtaaagtt gggctcgtag aattagctaa tggaggaact
      781 ctttttttag atgaaatagg agatatacca caaaatctac aggcaaaatt tttgcaatta
      841 atacaggata gaacttttac tccagttggg agcttaaaaa ataaaaaggt cgatataaga
      901 attatatctg ctacaaatgt tgatttagtt tcaaaagtaa aagaaaaaaa gtttagagaa
      961 gatttatact atagattaaa tgttatagaa ataaaattgc ctcctcttag agaaagaagg
     1021 gataatctag tggaaattat aaaatattat tttaataggt actcaagtga cttcaatcta
     1081 aataaaacta tttctaaaga agctatggat gctattgcaa attatagatt cccaggcaat
     1141 ataagagaat tacaaaatat aattcaaaaa atcttgctta cttgtacaga taatcatata
     1201 acaatagatg acttaccaaa cattcttaca aaaaatatac atattaccaa taatggaaat
     1261 aaaacacata tatcccaaat taataaggtt attatatctg actcaaaatc tactaattat
     1321 aaaaataaaa attttgacac tttaattaaa gagtatgaaa aaaatataat tttggatgct
     1381 tatgaaaaat ttggtagttc ttataaagta gccaaacacc ttgaaatctc acagtctaaa
     1441 gccaataggt taattagaaa gtatactaat acttaatttt aaatttgata aataaaaact
     1501 taacactatt tacatattgt aaatttggcc atttactgtt attatgaata tggttgaaat
     1561 tatttaaata aatactgttg acttagtttc atatatttac cattaaactt ttatcataaa
     1621 tatcatatca taataatata tttaataagt ctaataaact atacgaggtg taaatatgtt
     1681 aatgaatacc cattttatta tggcaaaatc aatacttgat aatatagatg aaaataaaac
     1741 attctttata agcgaaaaaa attttatata tggcaatata aagcctgatg ctttttctaa
     1801 atataaatta aaaaaacatt atttagatga gtcatttaat atgattgttt ctaaaataaa
     1861 ctatctatgc agtcttaata tagatagtct ttctaaatac ttttcagtaa gtagacttag
     1921 tcaggaacta ggagtcatat gtcatttttt atgtgatttt ttctgtgtag ctcacagtga
     1981 acgttgggag tttaagcata gcttaaataa acatgttact tatgaaagag aacttggagc
     2041 atttgcaaaa gatgttgatt tatctaaaat taaatgtggt actataaata gtagttttga
     2101 gaagtttttt actaaacttt atagcgatta caaaaagaaa actgattata tgaatgattt
     2161 aaaattttca gcttatactt gtaatagtgt cataaattat atacttgact ctattttatc
     2221 taatacaata aaatcatatc atatagcaaa ttgcggttaa cttaagaatt tttaaactta
     2281 tataaactta taattgaata taattaaaat gactgctcta tagaatataa ttttatactt
     2341 ctctaggaca gtcatttttt atactaacta tttatctcca ttataacatc tatggcactt
     2401 tttacactat ctactacata atttgcactt tgctttacat cttcccttgc cttagacatt
     2461 gcaaaactat atttaaatcc ttctaacatg caaatatcat ttggagaatc cccaatagta
     2521 gcaacttcat ctggacttac atttaacgtt tttattacat gctctatact tcctcttttt
     2581 gacacacttt taggaactat atttaaagta tatgttccag aaaaatatat ttctaagact
     2641 tcactaaact cactttttag ataaagttct attctggata actcttccaa attttcagaa
     2701 catacgccta tatttcctat aagattatca aaactacttc ttttttcaaa gtctgggtca
     2761 actataaagt cactagaata atgtctaaaa gcagtggtat cactacccac aattacatca
     2821 cccttatatt taaaataaag ttgtgcatca cttgactcaa atctatcaat gacttttttt
     2881 actaaattat catctatcgt tcttttaaaa attacattat aatctttatc atatataaat
     2941 gaaccatttt cacatacata gtatccattc atatctaaac tatctttgat aatattatgt
     3001 gcttgtttaa aaattctacc tgtagcaaca ttaaactgta ctcccttttt ttctatttct
     3061 tcaattgcta ctatatcttc ttttgttatg gttccatttt catatagagt tccatctaaa
     3121 tcacaaaata tatgttttat cataaattaa aagcctccaa aaacaatttt atattttact
     3181 aatactttaa tcaaaataac ttaaagcttt tctaattgta ttctcaactc attaagcaaa
     3241 tctctagtag ctcttaatct tatctttctt ctatccccat taaatatatt tttctttact
     3301 attgtttttc catttatata tattgcagta taaaccagtc ccacaggctt ctctttggtt
     3361 cctccaccag gtccagctac tccagttgta gatataccta tattagcttt aagtccttta
     3421 gcaacgccct cagccatttc tatagctgtt tgctcactta ctgctccaaa ctcttccaaa
     3481 gtttctcttt ttactcctaa tcttttcatt ttagaatcat tagaatatgt tacacaacct
     3541 tcaagaaata cagaggatat acctggatag tttataagag atgaagaaac caaacctcca
     3601 gtacaagatt cagccactgc tattgttaga tttttttcta caagtagctt agaaacagca
     3661 tcttctaaag cagtctcccc ttcagatatg gaaatatctc cttcagtata tacatattta
     3721 ccaactctac tttttatttt ttcttctact ggttttatta atttaaaagc ttctctttca
     3781 ttttcagcct ttgcagttat tcttattgta acttctaatt cttttgcata taaagcaact
     3841 gttggattag tctgctcatt aattatatcg agaatttcta attctagatt tgactcacct
     3901 attccatata atcttaaagt cttagatact agcatttcat ttgtgaattg ttgtaagtaa
     3961 ggtttgacac tctcattaaa catagctttc atttctcttg gaggtccagg caatactatt
     4021 ataaatttac catctttttt taaaattgcc cctggagctg taccattatt attttttagt
     4081 actatagcat cttttggaaa gtacacttgt ttcatattat tttcggccat ttttttccct
     4141 gttttaacaa agaaagattc caaaacctct agtgaaggtt tatgtagttc taatttctga
     4201 ccaaagtatt ctgcagcagt ttcttttgtc atatcatcac cagttggccc aagaccacct
     4261 gtagtaatta caaaatcact tctctttaag ctttcatcaa aacattctaa aagtctttgt
     4321 ttgttatctc caacagtact ctgatggtat acttctattc ccaatgatgc aagttcttta
     4381 gctaaaaatt gagaatttgt atttactata tctcccaata atatttctgt tcctacactt
     4441 attatttcag ctttcaaaat ttatcactcc ttgatttata gccattaaga ctagtcttaa
     4501 gttcataaca ataattttag caaaaataat aacataaata aagttaaaaa atcatttaaa
     4561 catatcttta aactagatta ttattctaaa accacacata atataaactt cgtatatagt
     4621 ggtactttca tcctcaactg ttaattcata taattaagta tgtaaataaa aagtttactt
     4681 taaaataata aaaattaaaa taggagtgta taatatgaaa atagcagtta taatgggtgg
     4741 aatatcatca gagagagaag tttctttaaa ttcaggaaaa gagatatata ataaccttga
     4801 taaaaataaa tatgaagttg taaaagtaat aatagatgat aaaaaagata tatttacaaa
     4861 gataccagaa gatatagatt ttgcaatact tgctttacat ggaaaatttg gtgaagatgg
     4921 atgtatacaa tcaatacttg agactatgga tataccatac tctggatgtg gacctctttg
     4981 cagtgggatg tgtatggata aaaatataac taaaaaaatg cttagagatt caaacctacc
     5041 tactgctcca tgggttttag taaaatcagt tgatgaaata gactatgatg aaatagataa
     5101 tataggttat ccagtattta taaaaccaaa ctcaggaggt tcaagtgtag ccacattctt
     5161 tattcattct aaggatgaag ttgaagaagc agttagaaaa ggtctggaag ttgatgaatt
     5221 tgtaatgatt gaaaaatata taccaggtgg agaatatact tcattcatat taaatggaga
     5281 agtgttccct acaatatcta taaaatctga ttcaggattc tttgattatg aagctaagta
     5341 ctcagttgaa aaaggtgcaa aggaagaagt tgtttactta gatgaagaac ttcaaaaaag
     5401 agtaaacgaa atttctgaga cttgttggaa aatttttaat tgcaaagcat atgtaagagt
     5461 tgatatgatt ataagtgaag gtattccata tgttcttgaa ctaaatactc ttcctggaat
     5521 gactcaaact agtttaattc ctagaagtgc tgctgctaga ggtattaagt acagtgaact
     5581 attagataaa cttatagaat actctttaaa ttagatgaaa taatttgagg tggtaatttg
     5641 atattttcaa atctaaaact aaatgataat gaaccaattt atatacaact taaaaattat
     5701 ataagtgata tgatatcaaa aggactaatt ccagacaata gtaagctccc ttctacaagg
     5761 gagcttagtc agcttttaca agttagccga aattctgtag ttttaactta tgaagaatta
     5821 aaatcagaag gattaatcta ctctatttca ggaaaaggta catttgttaa atcaaagaat
     5881 aagtcttcaa atactacatg gtcattaaac tgggattgtt tagagaatac atactctaaa
     5941 aaagccaatg aattggacat tataaaaagt gaaattcctt ggagctctga cctgatttcc
     6001 tttaaaagta tttctccaga tggagattta ttcgacatag aagaattaaa aaaatccttt
     6061 ttaaatagaa tttcactaga aggacataaa ttactcaact atggatatgc acaaggttat
     6121 aagcccctta ttgactattt actagaatat atgacaaata agggtgctga tataagtaat
     6181 aaagatatac taataacaaa tggattcaca gaaggctttg atattattat gtcatctttt
     6241 acccaagaaa aagactacat aatatgtgaa aatcctactc ataatactgc tataaaaatt
     6301 atgaaatctc ataacctcaa tatcttaggt gtagatataa atgaagatgg tttggacttc
     6361 aatatgctag aagacaattt agttaagtat aaaaataaaa taaagtttgc atacattaca
     6421 ccctcttatc acaatcctac aggaattgta atgactcctg aaaataggta caaattctat
     6481 aatcttatga aaaaatacaa tatagcaatt atagaagatg gatttaatga agaattactc
     6541 tacaatagtt ctcatatatt tcctatctgt tccctagata atatgaataa tggggtcgta
     6601 tatattggta gtttttcaaa aattttattt cctggaatga gaattggatg gatatttgca
     6661 gataaaaaag taatcaataa gctagaaagt gtaaaaaggt gcagaaacat acatgtatcc
     6721 tttttggacc aaggaatttt atttgattat ttaagtaatg gaggttttga aaaatacatt
     6781 aaaaaaatac gtaaattcta tggggacaaa tttaactttg catataagtg tataaaaaaa
     6841 tatataaaaa cagaatatat cttgggtgat ggtggtttgc atatatttat aaaactaaaa
     6901 aatatagata ctagagtttt attagaaaaa tgttatgaaa aaaatgttat atttatgcct
     6961 ggagatttat tttttacaga taactctggt tttgatactt taagactcgg attttctagg
     7021 ttgtcttttg aagacattga aattggaata aaaataattg gagaaactat aaatgaaatg
     7081 ttctaggtta actaaatatt gttagcctag tttattttta tgaggtataa tatatatagt
     7141 ttatatgaat ctaatactgg gggataatat gagtaaaaag tcaaaacgaa gaaaaattaa
     7201 taaattaaaa ttaacgattg taataactgt attatttctt gctttattat ctatatatga
     7261 gatactgttc tctaatccca acaaaaaagg taatataaag agtcaagaaa ctatgcaaaa
     7321 taaaaataat acaacagatg attctaaaaa taatcaaagc tctaccaaaa agcaggaaga
     7381 taataatatc aagcctactt tttcatattc ttcaatacca gataatataa aaaataaaat
     7441 gattggtaaa tctatgccta ctgatgaacc tataagcttt gatagtctat cttatttaaa
     7501 acttacatat tatggatttg atgaaaaaac tcatcaagga gaaatgatag taaacagtga
     7561 actagctcca gaagtagtgg atatttttaa ggaattatac gaaaaaaaat atccaattga
     7621 aaaaattaag ttaatagatg actatgatgc tgtagatgaa aaatctatgt ctgataataa
     7681 tacctcatca ttttgttata gaactatagc tggtacaaat gtagtttcaa atcatggtaa
     7741 aggacgtgct atagatataa acccattaca gaatcctcaa gtttctggaa atgatgttac
     7801 acctaaagta agtacagttt atgctgatag gtctagcaca aaatttggaa tgataaaaaa
     7861 aggtgatgat tgttataatg cttttgtaag cagaggttgg agttggggtg gctattggaa
     7921 aaacccagat tatcaacatt ttgaaaaata atagattaga aataaaggtg aattatatga
     7981 atttaatgac attggaaaat ataagtaaaa gctattctga aaaaaaatta ctagaaaata
     8041 tatctcttgg aataaatgaa ggggaaaaaa taggacttat tggtgtaaat ggtactggta
     8101 aatctacctt actcaaaatt attgcaggag ttgaagaaag tgagactggt aatataacaa
     8161 aaactaatgg tattaggata gaatatctac ctcaaaatcc aacttacaat gaaaattcaa
     8221 ctgtacttga acaagtattt aatggaactt ctgaagaatt aaagctactt ggtgaatatc
     8281 aagaaatcct agaaaaactt aattcaaact ttacagagga attaaataaa aaattattat
     8341 cagtacacga aaaaatagat gccttaaatc tttgggattt agaaagtgaa gctaagtcag
     8401 tacttacaaa gcttggtata aatgacttta atcaaaaaat agcagaactt tctggtgggc
     8461 aaagaaagag agtctctctg gcatcagccc ttattacacc ttgtgagttt ttgatactag
     8521 atgagcctac aaaccatcta gacaatgata ctatagattg gcttgaggaa tatcttaatt
     8581 ccagaaaagg ctcattgctt atgataactc atgatagata ctttttagac cgtgttacaa
     8641 atagaattat tgaactggac aaaggtagat tattcagtta tgatgggaat tattcaatat
     8701 ttttggaaaa gaaaatggaa agactttcgc tagaagcaag tattgaagat aaaagacaaa
     8761 atttaataag aacagagctt gcgtgggtaa gacgaggagc tcaggctcgt agcactaaac
     8821 aaaaagctcg tttacaaaga tttgatgaac ttataaatag agattctttt aaacctgatg
     8881 aaaatcttga tatatctgtt gcatcaagta gacttggaaa taaaataata gaaatacaca
     8941 atatatctaa atcatttgaa gaaaatactg ttatagataa tttagaatac actttaactc
     9001 gtacagatag aattggtata ataggcaaaa atggtatggg taaatctacc cttataaata
     9061 tacttaatgg agaaatagag cctgatagtg gatatatttc aattggagaa acggttaaaa
     9121 taggttgttt ttctcaagac gactctcata tgaatattga tatgagggct atagactatg
     9181 taaaagaagc tagtgactat atagaaactt cagatggtac taggattaca gcttctcaaa
     9241 tgtgtgaaag attccttttc aatggtacta tgcaatatac tttgataggt aaattatctg
     9301 gtggtgaaag aagaagacta catcttctta aaatccttat gacagctcct aatgtgcttc
     9361 ttttagatga acctacaaat gatttagata ttgaaacgct taaaatatta gaagaatacc
     9421 tagacgaatt taaaggtgta gtagttacag tatcacatga tagatatttc ttagatagaa
     9481 tttgtaataa aatatttgca tatgaaggta atggaaaaat tcatatctat acaggtaatt
     9541 acagtgatta tcttatttat agagagattc aaggaattga atttgaagaa tctaaaaagg
     9601 aatcagttaa aaaagatgac tctactaccc taaaaaaaga aaaacctaaa aatgataaaa
     9661 agcttaagtt ctcatataat gaacaaaggg agtttgaaaa tattgactct gatatagaaa
     9721 aactcgaatc taaaattgca acacttgatg aaagtactgc aaaattttca actgatttta
     9781 ctaaacttca agaaatttta gatgagaaat ctaaactaga aaaagaactt gaatacaaat
     9841 atgaaagatg ggaatattta aataatctag ctgatgaaat agctaataat aagtaatatt
     9901 aaaataatct taagtttaat aagttttgct ttaaatctgt aatttataaa tattttaaag
     9961 gaagtgtctc aaaatgaact ttttagtttt atgagacact cttttttatg agaaatcaaa
    10021 tatattttca tcatttcaac aatacaaaag aggcttatct ctattttgag acagcctctt
    10081 tcataattac cttctaattt atatgctcta atatatctat aataattctt attatatcta
    10141 tatatccagt ctttcaatat aatgacaatt aacttcaata tgatttaaaa atactaacaa
    10201 cctttcataa agtggatgag attcttctcc atatttagct tctaggttct tgcctatttc
    10261 tctaacagtc ctttgaccat caatttgtaa aaaaacaaaa ctggaatatt catctagttc
    10321 tatttttttg tatttaggta ttctagcttt taactttcta aaaaactttt gaattttatg
    10381 gtcctgtttt tcaagtatag taacaactct attttcagct acttcatatt caagtccatc
    10441 agtaatttta aatacaatat ctaaaacatc attactcttg ctcattattt tttactttag
    10501 ataaaacaat aggtaatgct gtaagcacaa ctaaaactac taataaagca attgccattg
    10561 aatttgttgc agcaaatcca cttggtacct ttggagttac aactccagtt acttgtaata
    10621 ttatacctat taaaccaatg atagaaccac cagcaacaag accagaagat aaacttatac
    10681 cattagaaac tttaacttct ttttcttttt cagttttaga tactaattca acaaataaac
    10741 gaattaatgc tccaactaat attatagaag ttgttgcaat tggtaaatag aatcctatag
    10801 ctatagtcat tattggtaaa tttaatgcat atagaactat tgccataaat actccaacaa
    10861 taatcataac ccaaggtaaa ctacctgaca taataccaga agttaatgtt gacattaagt
    10921 ttgcttgtgg taatgcaaat tgtacattat caccagttgc tctaagttgg tcagaaagta
    10981 ataaaattac tcctacaaca actataacac caactatact tgcaattgta aagtaacgtt
    11041 gcatttcgtt tttgctacca ccaataatat aagttacttt ttgagattga gtataacctc
    11101 cagctatagc aatagcaaca actataaatg aaccaaataa aagtaatgat ttattagctt
    11161 ctaaatcagt ccatcccatt ataacaaaca ctaatgttac aataactaga gaagctatag
    11221 tcataccaga aactggaaga tttgatgttc cgattgttcc tgttaaacga ccagctacta
    11281 tgacaaataa taaagataat aaaagagata tgatagctcc aataattgcc ataacaatgt
    11341 taccagaaat taagaatgct gctaaaaatc caataactac tccacctaat aaaagtatca
    11401 tttgtataga tgaaccttct tctccttctc cagcacttga tttagcttta agtgtttctt
    11461 ttatagaagc gattatagtt ggtataagtt ttattgcacc aataatacct ccacaaagca
    11521 tcataccagc acctatatat tttacataac tactagatat tgctccaaca tccatttggt
    11581 taagaggcat tgcaggatta ttccatacca tagctccatc tttagccata tctgtaaagt
    11641 atcctattaa aggtgctata ccaaagtttg ataaaatact tcctgcaaac attgttaaag
    11701 aaacttcaag ccctactata aatccaattc ctaaaagtag aggatttact tcaatatcaa
    11761 atttccattt gtagaatttg cttcctacaa gactcattac attgtttgct acatttaaga
    11821 atgaaccagt taatacagta ataaatccac ttattacaaa tccaattccc atgtacttaa
    11881 ttgaatctcc accagcctct gaagcaacaa gtgtttcaga tatagccatt gattctgggt
    11941 acataagttt accatgttct tcaactatta gatagttgtg aactatagca gcaattccta
    12001 taccaaataa aactccacca actccaacag ctaagccttc aaagaatgta atttggcttc
    12061 caattaatat aactgctggt aatacgaaaa taaatccact tgcaacagat tctccaccac
    12121 ttgacatacc ttggattaag tttttaccaa gaataccctt tgaacgagca aatgctccaa
    12181 caaaagcaga acctattata gctcctggaa tacctgcggc aactgtaaga ccggctttca
    12241 ttccagagta agttgtagat gcggcaaaaa tggcagctaa aataataccg ataatcagta
    12301 cagctacatt tccaccggtt ctggacttat ctgtgatata tggaacgtaa tcttttccac
    12361 taacctcacc ataagcaccc ttaggtaatt ttttgttcat aaaattttca ccccttttca
    12421 atttgtatat attataacat atatacatat aaaacttaac tttttattat tttacaaata
    12481 gcgagtcaaa actactaaaa tttaacccta tattattaat ttctactttt agatattttt
    12541 atgctaattt tctttttatt tttcaattct actaaaaact attaagatat aaaataaaaa
    12601 aattttttaa tgtaaaatta attttgtaaa aaaaatgctt taaaacacgg aaaagtataa
    12661 aaaatacaat aatcattctt ataaataaat tttcaccata gtaaatatat acagaaaatt
    12721 cttgttttta tgcaaattga ttcttttata aaaataagtg tcataaagaa atgtctgaaa
    12781 tcactttatg acacctatca taataaaaaa ttattctggt aatgctcttt tatttacttt
    12841 tccgattggt gtaactggta tcttatctat cctcattatt attgttggaa tcatatatgg
    12901 aggtagatgc tcatttaaat attctctaat agttctttca tttatcttac cactatcttc
    12961 ctcatcaatt acataatatg cacatatata catatctcca tcatcatttg ttctcgatat
    13021 acaaacaaca cttttaattt ctgtaatatt atttattata ttttcaatct catatagctc
    13081 tatcctaaac tcatcaattt ttacctgatt gtccatcctt ccaatataat ctaaatttcc
    13141 atcatctaac catcttacta aatcaccagt tctatacatt aacttattat gttcacaagt
    13201 tttgtaagga ttctctacaa atacttgctc tgttagccct tttttattta aatatcctct
    13261 tgatatttga atgcctgata gacatagctc tcctggtgct ccaattggac atagtttgtt
    13321 ctttttatct aatacatatg cataagtatt aaatataggt tttcctattg gtatttttcc
    13381 atattctctg tccacatgaa aagaagtcga tacaacagta aattcggttg gtccatactc
    13441 attatatagt tcataattac gctttctata tccttttaat ctttctcctc cggtctgtac
    13501 aactctaagt gaattattat tgcaattttg tataaataac cttgcaaatg ctgttggcaa
    13561 tgctagtata gttatgtcat tttcctcaca gtattcattt actgtatatt catcaaattt
    13621 agcttcttca ggcataatat ctattgatgc acctacaagc aaataaggga atagttgttt
    13681 cattacagca tcaaaactga aacttaagta tataccacat atatcatttt ctgtagcgct
    13741 tatagatttt acgctataat aacacatatt tactaaactc ttatgttcta tagctatggt
    13801 ctttggccta ccagttgacc ctgaagtaga tatcgtatat gcacaatttg atgatgttat
    13861 tttttcagtt aattcatatc caagcttaaa catttcttca tcatttatat cgagtactat
    13921 ggtattctca tttacaacct tacttttgag ttcatctgta gttatcacta cttttgcatt
    13981 actatcttca agaaggtaat tcaatctttc ttctggatta cttgtgtcta ttggaaaaaa
    14041 tgctccccct gatttcataa tccctattgc tgtaataatt atgttaatat ctcttggtaa
    14101 cattacagct actatgtctt cactttccac tcctatatga tttaaataac ctgcaagttt
    14161 atcagttact tcatccaatt ctttatatgt taactttcta ttttcatccc tagcagctat
    14221 attactaggt gttaccttaa cttgctccct aaataaatca acataacttt tatcatatgg
    14281 tatttcatat tgattattat taaagtcttc caataatact ttttcttctt ccttacatac
    14341 aaattctaaa tcttctatta aaatatcatt atcttctatt atttgctcta ttatataatc
    14401 aaattgattt aaaattcttc taatagtttc tcttttatat atagatggtt tatactttat
    14461 ttttatttta agtgccctct tagtatatac tattacagat aaatcatatc cttttttttc
    14521 tatgtcataa tcatatgata aatcaaaact tttagctata aatattgtgt ttataagctt
    14581 agtatttaga tgatttttat tctctatatc tgataacaaa tagtatccat actctaaaga
    14641 cctgctccac tgccttttta gcttccttac aacatctata aatttttcac tttgtctaac
    14701 tcttactctt attggaatta tattcattgt tttaccaaaa actacatcat cttcatatat
    14761 atacttttga agtataaggc catatatagt ttctatgata gagtctatgc taatattgtt
    14821 cactttagaa tattgaatta acctttcatg tttctcaata ttcatcttaa aaacctcatc
    14881 tactacttca tcagaacaat ctttataaaa aaaatcattt ggaatttttg ctatattcgt
    14941 ataatcatct aacaaatcca tccaataatt catagctcgt tctaaattaa cttcatacat
    15001 atcaaaattc ccccttacta ctaagtctat agaaaaaatt cttttggaat tttattttca
    15061 caatatataa atatattaat aatacatatg caagctctaa aaacatgagc ttgcataaaa
    15121 ttacacttat tatgactact aaattcgtaa tatttacaat gtttaaaata tattataaca
    15181 c
//
LOCUS       DAMKMI010000099        15139 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMKMI010000099 DAMKMI010000000
VERSION     DAMKMI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.103"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(157..366)
                     /locus_tag="PC429_002662"
     CDS             complement(157..366)
                     /locus_tag="PC429_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848160.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFESIATLVRGGSSWSFNFIV"
     gene            complement(563..892)
                     /locus_tag="PC429_002663"
     CDS             complement(563..892)
                     /locus_tag="PC429_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848161.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVF"
     gene            1019..1402
                     /locus_tag="PC429_002664"
     CDS             1019..1402
                     /locus_tag="PC429_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848162.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            complement(1771..1980)
                     /locus_tag="PC429_002665"
     CDS             complement(1771..1980)
                     /locus_tag="PC429_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848163.1"
                     /translation="MMGLKLKRRYKNVLIYINIRTIININCKIKFIELYLTFNELKNK
                     LANNEKTTVINENNKAFSGLVDFSN"
     gene            complement(2622..2873)
                     /locus_tag="PC429_002666"
     CDS             complement(2622..2873)
                     /locus_tag="PC429_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848164.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            complement(3119..3274)
                     /locus_tag="PC429_002667"
     CDS             complement(3119..3274)
                     /locus_tag="PC429_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF3848165.1"
                     /translation="MALEVKIEVKIIKDVVILNITHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     gene            3953..4177
                     /locus_tag="PC429_002668"
     CDS             3953..4177
                     /locus_tag="PC429_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848166.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRE
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     repeat_region   4464..5217
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4529..4557
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
     gene            complement(6095..7122)
                     /locus_tag="PC429_002669"
                     /pseudo
     CDS             complement(6095..7122)
                     /locus_tag="PC429_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
     gene            7313..8134
                     /locus_tag="PC429_002670"
     CDS             7313..8134
                     /locus_tag="PC429_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3848167.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIKIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            8259..9776
                     /locus_tag="PC429_002671"
     CDS             8259..9776
                     /locus_tag="PC429_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3848168.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            9799..10083
                     /locus_tag="PC429_002672"
     CDS             9799..10083
                     /locus_tag="PC429_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3848169.1"
                     /translation="MCNEISLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPRER
                     EIVQLRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(10180..11844)
                     /locus_tag="PC429_002673"
     CDS             complement(10180..11844)
                     /locus_tag="PC429_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF3848170.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQVLIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRIGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(11916..13163)
                     /locus_tag="PC429_002674"
     CDS             complement(11916..13163)
                     /locus_tag="PC429_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF3848171.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            complement(13150..13824)
                     /locus_tag="PC429_002675"
     CDS             complement(13150..13824)
                     /locus_tag="PC429_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF3848172.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            complement(13996..15075)
                     /gene="mltG"
                     /locus_tag="PC429_002676"
     CDS             complement(13996..15075)
                     /gene="mltG"
                     /locus_tag="PC429_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF3848173.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
BASE COUNT         5256 a         2298 c         1732 g         5853 t
ORIGIN      
        1 ataaaattca tatattaaga aatattaata gtaaataagc aattgttgct ataacttagg
       61 aattatcttt aaaaacacaa attaatttac attttctaat tttaaagtat atgtatatct
      121 ttaattttaa taaaattcac ttaaaatatt ttaaggttaa acaatgaaat tgaatgacca
      181 agaacttcct cctcttacta atgttgcaat tgattcaaac ccacaattgt attgaactga
      241 atctctacct actggtttcc aactagaagg tgcattatat gagaaagaat ttgatgttgg
      301 ctctttcttt tgttgctgct taattcctag cccattagaa ataaatccag tttgatttat
      361 atgcaccctt ctatttgtta cacgaatctg gccgtctgca atagaccagt ttccagaaac
      421 tttactaacg gaaatatatt gaaatgctcc atctcctttt gatattgtat atgtaactgt
      481 accagatgct tttacaccac cagttttatc ccatccatct tctgatatac ttgcttttct
      541 aagaggcttt attgtattag tatcaaaaaa ctgtcttaga atatatcttt gatgttaaat
      601 ttttagattg tttaacttct aataattgag ttgttgatat agaatcaacg tatatggact
      661 ctcctgttct tgcatttttg attatacctt tttcattgga acttttaaca tcagtaattc
      721 catttttagc catatcgtat aatttgtcca tgtctgttac ttgtttctct gaatacatta
      781 ctttcataga tgaatcttct accttactag cacttgcatt tgttggtaat agtattgtca
      841 ttatgagtaa agataaaata gttgttacag atttttttaa cctataattc atattaatcc
      901 ctacttttta tttgttataa cagacattct attgtaatag aatataaatc cacaatattt
      961 ttaaaattag tattgaataa ctattctcat gttataatta ttcaaaggag ggattgatat
     1021 gaggaaaaaa ttttttatag gtattcttat agttttaata atctctgtga tagctatact
     1081 taatataaaa aatgataaaa atgtctttat tggagaatca gaaaattgga aagcgaaatt
     1141 tgtaactact gaaggacact caaaattaga aataaaatat aaaaatagta aagatgtagt
     1201 tgaacaaaaa tttaattatt cctatcaatt tatagatggt gaagatgcag ttgtagttgt
     1261 aaaaaatgaa attttagaag caggtcaaga aaaagcaata aaaatacaaa gaccaggtct
     1321 ttattcaaat agagaatcta gttacaagct aattatatct tggaatggaa atgaggaaac
     1381 tataaacttg aaaaagaaat aaccatatta tcaattttta aaaaattatt attcaaattc
     1441 ttataaaaag agtgtttaga aaaatgaaac actctttttt gtataagttt ttgaaaacta
     1501 taaatgaaat atatcaaaat cgttcgaaaa attactgaaa taaactctta aatatattat
     1561 aaattaaggt atattataca tgaatttatt gggagtttat gagttttaaa attggaaacc
     1621 aaacaataag ggcgaaaaat acttcaagga aaatatataa agttctataa attatcaaaa
     1681 atatttcgca aaaactgata attattacaa ataaattgat aatactaata atattagaaa
     1741 tatcgaaatt gaacaaacta ggattaaagc tcaattagaa aaatccacta atcctgaaaa
     1801 tgctttgtta ttctcattta ttactgttgt tttttcatta tttgctaact tatttttcag
     1861 ttcattaaat gttaaatata actctataaa tttaatttta cagttaatat ttattatagt
     1921 tcttatattt atatatatta atacattttt atatcgcctc ttcaatttga gccccatcat
     1981 tatataaaat taacctactg gatatcttaa tttaatatat gtacactatt catatcttaa
     2041 tagtcaatat tattcatttt taatagctct ttttagatgt cagaattatt atattttgaa
     2101 atttattaaa tttttgcatt aaaaaaacta gaaattaatt taagtcttga attacttata
     2161 actttctacg tatctacctt tatattctta ctctgatttt atatctaata tcaactcttc
     2221 atcaaagcta ttaaatatac ctctaattga gtcaattatt tcacttactt cacctttaat
     2281 ttttatatta acttcaacca tttcaaatct catcttttta tcttataatt tctataagtt
     2341 tttttattta ttgtgttgct tactttgacc agcacttttt tatttaatag tctaaatgat
     2401 attttttacc agttttaata tccttaaata ctatatttac taaaaactta taatttcttt
     2461 taatacatag ttaatacatt cttattacta ataacaatac tcaacaattt tatcgccttt
     2521 tttcaaatat gatttaactt ttttattcct aattatttaa aaatagttgt ccaattttct
     2581 tctatgagaa agtgctaagt attagcaact tctctattct cttaactttc tactgtctca
     2641 actttcttca tagctatatt aatagctact tctttagcta cttcattgaa ctcttcccat
     2701 cttctcctat tttcttcatc agatatacgt ggatatacta tataaacttc tgtgttttga
     2761 ttagctataa ttttttcatc atattctata tttttttctt tatcatattt gtatggttta
     2821 aattgttgta gtattttcat aacatcaccc cttgttataa aatatgaact cataaacttg
     2881 tcttatactt atttaattgt tacaattttt acttaaaatc actttatatt ctatatatta
     2941 aagctaaatc ctttacaaaa gtaacatatt cttctataag tttcttatcc tcttatatag
     3001 tatctagttt gtttaactta tctcttttaa acttacatac gccttcttct gccattcgtc
     3061 ttttattaat tgttaacatt atttcaatag acacaccaaa actcttgtta agaagctctt
     3121 agctctcatt agtaaaatat tttacataac ttaaatcacc ttgacctcta gcaattttaa
     3181 ctgttaatag acttgtttct tttttccaat gtgttatatt taatataaca acatctttta
     3241 ttattttaac ttctattttt acctctaatg ccatattatt gcttgattaa tttgtatcct
     3301 taaatattaa attttctatt aatacatctt ctatacagct tagtttctct cgcttaatct
     3361 tgaaatatat tttctctaaa ttgtcaaatt gttctcaagt tttatcaata tctaatattt
     3421 tgcagtgtct atttgcacat ctttcggtcc aaatgtataa ttgactagaa tgttttgaaa
     3481 tcaactgact attagtaggt tagttcttaa attctttcaa atactcgaat tgtaataaaa
     3541 cataatttac attctcttta tttctattga agctagttta tatattgttt acatcatttt
     3601 catatatatc tgctagttgc tatgtattta aaattctctt attatttctt tatattactt
     3661 gtaaattatt catccttatc atcctcttaa ttaaattttc tatatcataa tcaagtatct
     3721 tagatagttt ataggtaata tatcaactct aattgaatat taccctttcc tgtacttaaa
     3781 ataagttgat gcatttttaa aacctaactc ttttgcaaca ctttgtaagt ataaatttta
     3841 tgtatgtctt cttttttata aaatcatagt gtatcttttt catatattca cattaatttc
     3901 tatatttcaa taacaatatt tccaaagcag aaacttttcg agataattat gtatggatgt
     3961 aaatcaaagg attaaatata ttagggaaaa tatgaatagt tctcaaaaag aattgcttga
     4021 aaaaacaaat ataaatacaa gtgttatgaa tagaatagag tctggtgaaa gaccaacaag
     4081 agaagatggg ttaataatat ttgctaaaat ttttggttta tatactgatt atattcttgg
     4141 tttatcagat acagaaaaac taaatattgg caattaaaac tgaattaatt gtattatatg
     4201 tgtgatgaat ataaatagag aattttttga aaagcttgaa ataacttaga aaaaataaga
     4261 ggttcactca agaacaagtt gctaaagatt aaaaaacaac aaaggtatct actaaaagat
     4321 atgaaaatag tttaagagag ttgagtctgg aaatatttat gtgtattctt taaataaaaa
     4381 tttatttttt aaatgataat taaattttta ctgatatggc tatgctttta aaagtcctaa
     4441 ccttattttg acattatatc tgtctttaca taccacttaa ttaatataaa acagtataaa
     4501 aattagttac agatgtatct tttttgttct ttacatacca cttagttaat ataaaacttg
     4561 ctgtaaggct gttcgttaca gggatggcag agtcctttac ataccactta gttaatataa
     4621 aacaataacg aagctagaaa tctttattca gcgattacaa gctttacata ccacttagtt
     4681 aatataaaac tatgcgtgaa atatttgttt aggaggttta tttttacttt acataccact
     4741 tagttaatat aaaactctca tgatatctgt aaacttttcc cataattttc cctttacata
     4801 ccacttagtt aatataaaac atagatgttt caatttttct ttgatattgt aaagctcttt
     4861 acataccact tagttaatat aaaaccatag ttcttaagtt cttcagaact attcaagtgc
     4921 aactttacat accacttagt taatataaaa caagacccaa ctggtactat gtaaacaatt
     4981 tatcctctct ttacatacca cttagttaat ataaaacttt aattatgaat gataatcatg
     5041 ttacagagaa acaaacttta cataccactt agttaatata aaactcacat attcttctat
     5101 ctatatcact taacacgtca aactttacat accacttagt taatataaaa ccaaatgaat
     5161 ttctaccaag aatagcaggt tttattgcct ttacatacca cttagttaat ataaaacccc
     5221 aaaataaatt gagtatttcc aacacttaca tctatacaat tctctcaaat ttgcagtgaa
     5281 ccacgagtag tgcaaatgat aatatttatc atataccctc aatgccttat gtttcaactg
     5341 ttaagcccta ttttatcaaa aatatcgctc actgcaaaac tatttataat attatgaata
     5401 tattattttt aaatatctgt accaatttgt agtataataa aatcaagaaa ataatttact
     5461 cctataaaga gtgattattt ttaagtgaaa atattatttt tttaaatcat tcttattggc
     5521 gtttgggaga tttttatttt atcataaata taaactgata taacaccagc tgacatgctt
     5581 gccactttag attattattc ttgctacaac tattataaca tataaaactt acttattttt
     5641 accaaatatt ttatgtaaac aataaatatt aacttataaa atatctgctc atttacagat
     5701 atttcataag ttaatagctc tataataaaa tttttatcat tgtttataaa aaattttact
     5761 ttgcattttt ttataaacaa cagttttatt tataatgttt tgtggtataa taaaagcaag
     5821 aagaactaca atctattttg cgttagagtg gagttcatca taaacagagt ttattttttg
     5881 gatttaaata taaatttaaa ttcaactcgt aagtcactct ttgtgcgaga gtggcttttt
     5941 gcttttttga atagtttact gattaagtaa actaccacac tagctgttaa actagctaaa
     6001 acattaaaca aaaagttatc catgaactca cctcccttcg aatcgttggg aggataatct
     6061 tttgtacatg aactccactc tatagattgt aagttaaatc ttcatcttct gaataattag
     6121 gttcgtaata aaatttcttc acacctaata gttttttcac tacattagca gcatcttcta
     6181 ctgtatattc tcttccagta ttagaatcgc ctatattttt tgttacattt aaacactgct
     6241 gtaatatact atcactcaaa tttcctatag cagttaattt agttgtactt ttcaatacac
     6301 ttttattact atcattacca actaacacaa tagcattatt tttagcaatg gtagaaccaa
     6361 caagagcata cactaaatca gtaccacttg ctatataaaa ttcttttact ccactataaa
     6421 atttatttat tatctttttg ttagtatcat atctatctgt cccacctaat ctagttgaat
     6481 tagtatcctt aactattttg tcattcattg atgaagtacc acctattaca taactttcta
     6541 caccagttgt attaaatgga cactttttcc atcagttaat actatagctg ctttatctct
     6601 aactgctaca ggtgcagcac tcattgcatc aggctctcct ttaaaagcat tagtcaatat
     6661 gactttattt actttgttaa ttgagtttat ttcttttgcg acattatagc ttgtcttaat
     6721 tctatcacta ccttgaagtc ttttaatctc tattcctttg ccttttagaa cagtttcagt
     6781 atatttatca atagagtttt ctcctccaat tatatatact ttttttgcct tttctaatct
     6841 tttaagtgta gcatttggta tgttattttt cttagtcaaa agaattggag cgtttatagc
     6901 tcctgcaagt ccacttgcac ttaatccatc agacattgta ctgtctgcat taattaatat
     6961 agctgtagta tagttttatt tgtctgcaat tataccagca gtttcgtatt tatctgcacc
     7021 tttaattggt tcaattttat caagagcatt tgcagatata gggcaactag caataaatat
     7081 agataaagat aaccctattg ttaataattt tttaattttc ataatatcta cttctcttaa
     7141 atattttcac ctaaattata taatactttt caggaaaatg ttaccataaa tttaaaaggt
     7201 tattattact taatataata ttaatgaaaa atatcactct taaattaatg actttggtag
     7261 aatatggtga taaaataaaa ttaatgagag acaatataag ggggattata ttatgataat
     7321 cagtaaaaaa ataacagcaa tattagtatc atttttaatt atgatatcaa tggttggatg
     7381 tgctccaaaa gaaagtgcta cagaagtagc taaaaagtac cttgatagta taaaaatcaa
     7441 agactacgaa gtagcatata atttattaag taaagacagt aaggaaagcg tcagtctaga
     7501 aaaatttgaa gaatatcaag atactttatt taaacttcaa accctaaaag attataaact
     7561 tggtgaagaa aataaagtag agaaatacaa atattctaat aaagagtaca caaatcttgt
     7621 taaaataaaa gaaactgata ctataaagta tatcactgaa aataaagaag atactgcaaa
     7681 ttttgataga tattttataa tcgaagataa tgaatataaa ttattatggc ataaagactt
     7741 taaaagagct cttagctcta attatgtcaa aatcgctagg tcagaaacag aaaaagatga
     7801 agataaggat tataataaag ctgctattga tatcaaaaaa gctatagaaa ttgatgataa
     7861 aaatccactt gcatattata cacaatcata tatttatata gagttacaac gatatgatga
     7921 ggctttaaag tcaattgata aatacttaga atatctagat aaaaataatg aagcttataa
     7981 aatagacgct agtgatgctt acaatttaag aggtgttatt tttatgagta aatcagaatt
     8041 taataaagct agagaatctt acaataaggc gcttgaactt aacccggata atgaatatgc
     8101 aaaaactaat ctaaatgata ttaaaggatt ataaggcatt aatattatag aaaataagta
     8161 tgttgaaagt taaattaact tttgacatgc ttattttcta tattttaaac tctattgatt
     8221 tactatataa tagtgcataa ggagtgtgat tacttattat gagtgttgcc atatacttaa
     8281 gaaaatcccg tgctgatgaa gaggcagaaa aacaaggcga atttgaaact ttaagtagac
     8341 ataaatcaac cctactaaaa ctagcaaaag aacaaaactt agatgtaatt gagataaaag
     8401 aagaattagt ttctggtgaa agtataatac atagacccaa aatgcttgaa cttctaaaag
     8461 aagttgaaga aaataaatat gatgcagtct tagtaatgga cttagacaga cttggtcgtg
     8521 gagacatgaa agaccaaggt atcatattag aaacattcaa agaaagtaag accaaaatta
     8581 tcacacctag aaaaacatac gacctaacag atgaatttga tgaggagtat tcagaatttg
     8641 aagcatttat ggcaagaaaa gaattaaaac ttatatcaag aaggatgcaa agaggtagaa
     8701 taaaatcagt tgaagaaggg aatttcatag gaacctctgc tccatttgga tatgatgctg
     8761 taacaactgg tagaaaagaa agaatacttg taccaaataa ggatgctgat gtagttagga
     8821 ctatctttga cttatatatt aatgaagata tgggttgtag taaaatatca aaatacctaa
     8881 ataacttagg cattaaaact gctacaggtg ctaattggta caattctgca attacaaata
     8941 taataaaaaa taaggtatat tgtggctaca ttcagtggca aaaaaaggac tacaaaaaat
     9001 ctaaaaatcc aaataaaata aaaacagtta aactaagacc taaagatgaa tggattgagg
     9061 caaaaggaaa gcatgaacct ttaataagtg aaataacatg gaaaaaagct caaaatatac
     9121 tcaaaaaaaa tggtcatgtc agctatggta atcaaataaa aaatccactt gcaggaatag
     9181 tgatttgcaa aaattgtggt agaccattag tatatagacc atatgcagac cacgactata
     9241 taatttgtta tcatcctggt tgcaataaaa gttctcgatt tgaatttatt gaagctgcca
     9301 ttttaaaatc tttagaagac actgtaaaaa aatatcaatt aaaagcatct gatatagact
     9361 tagataaaaa taataaagat agtaatatag aatttcaaaa acgagtctta aagggcttag
     9421 aaactgaatt aaaagaatta ggcaagcaaa aaaataagtt atatgattta ctagaacgag
     9481 gaatatatga tgaagataca tttattgaaa ggtccaacaa tataagttca agaactgaag
     9541 aaattaaaga ttcaattaag acagtaaaaa atagattaag tactgttaaa aaagacaatt
     9601 ctaaaataat agaagatatt aaaactgtgt tatcacttta ccatgattca gattcccttg
     9661 gaaaaaataa actcctaaaa tctgtaattg ataaagcagt ttattataag tctaaagaac
     9721 aaaaactaga ttcttttgaa ttaatggtcc atttgaaatt acatgaagac cagtaatacc
     9781 aataacatat aaagagtagt gtgtaatgaa ataagtcttt tagatatact tggtacagag
     9841 gctaactatg ttcttgatga agttgaacta aaagttcaag ttggaaaact ttacgaacaa
     9901 ctaaacaaaa ttcttactcc tagagaaaga gaaattgtcc agttaagata tgggttaact
     9961 ccttatggct ataagacaca aagagaaatt gcacaaaaac tcgatatatc aaggtcatat
    10021 gtatctagaa tcgaaaaaaa agctcttaaa aaactagaaa aagagcttgt tcaagaaagt
    10081 taatactata ttttaaaagg atatatcgta catcattaga caaaattttc tatctaaaat
    10141 caaagttacg atatatcctt aaattttata gtacataatt tattttatac tttcacatat
    10201 ttccttaaat ataggtgttg cagatttatt tcctttttga gttccttcta ctaacacagt
    10261 tattacatac ttaggtcttt cttcaggata aaatcctgtt atccatccat ggtctatagc
    10321 ttttttattt aaactacttt gagctgtacc agttttaact ccacaaccac cttcaatatc
    10381 ttttaggtct ttagctgtac caatcctaga tacagacttc atatattctt taacttgtgt
    10441 acacacataa ggagaaataa gttcttcttt ttttgaagac ttataagttt ttattgtatt
    10501 catattattg tccacaagac ttttatataa gtatagtggt ttgaatgttc cattattagc
    10561 tattatttga gtcatttgat ttatttgaag tggtgtaaat tctatatttt cttgacctat
    10621 agctaaattt cttattgata tgttttttgg agcttctcta attttttctt catcaagacc
    10681 aatatcaacc ttttcaaata aatgtaattt ttcagcagat ttgagtatct tttcttttcc
    10741 aagtttcata gctatatcta aaaatgcagg attgcatgaa ttagaaaaag cttgtcttaa
    10801 gttttggaaa ccatgaccat ctcttttatt acatcgcagt atttcattag tattcccaac
    10861 tttagtctta cctgtacagt tgtaagtata gttctcatct ataacaccat tttcaagtgc
    10921 agaaaataaa actaccatct taaatacaga ccctggtgga tatgtcgctt ttataactct
    10981 attttcaaat tctccatttt ttccttttaa actttttgat atgtcatttt ggtcaaaatt
    11041 aggtctagag cacattgcaa gaatttcacc tgtagatgct tccgagatta ttgctgctgt
    11101 aggattttct tctttattta gtatttgttc taattttttt tgaatgttat aatcaatagt
    11161 agtttttaga tgtctatctt tatccttatt atcaacagtt tttacacttc cctttagtat
    11221 gtttagagat ttttcatttc ctgcatctcc agctttaaaa gcacttatat acttttcatt
    11281 tgaatttcta agtacactat ccatactctt ttcaattcct gattgacctg atttatcatc
    11341 ttcttttata tatcctattg tatgtgataa taaaccatct tttgcgtacc tcatagtttt
    11401 ttcttctacc attattccat ttttctcaag ttcttttttc atactcttat cgatattttt
    11461 agtctgaatc tgtattatag ggcgtgtcaa ttgctcttgt acagctttat atatatctaa
    11521 atcattcatc ttagtagctt tcttaataag ctccaaaatt ttatagttat tatttaattt
    11581 ttctttttca acaattaata cctgagattt acttgtatct gtaagcttct tgttgtttct
    11641 atcatagatt attcctcttc cactatttaa ctcgactttc tcaacgcttt gtgattcaac
    11701 actttgtttg tatttatcac cttttaatac ttgtatatca actagcctat aaattaaaac
    11761 actataaatt attaaaatta tagtaaatat acaccagctt ctctttccca cttttttcaa
    11821 aaatggagtc ttcttttttg acatgaaatc accccttaga taatttcatt attgacaatt
    11881 tctaaggggc taatactatt tttatcaatt ttttattatt cttcaacctt ttcttctata
    11941 acttttctaa gcatataatc tttaccaact tttactgata gctttaactt cactatttgt
    12001 ctaggatgag gagctgattc tatagcttct ccttcttcat tgtacatttc ttcaatttta
    12061 gtaaacatag tttctttata tggtcccata acttctattt catcaccaac acacatttta
    12121 tttctttgct caacaacaat aagaccatct tcatcttctg tttctctaac tatacctata
    12181 aaatcatagt ttctaacata tgatgctgat tgataatttt ggtcttctgt tgttggttta
    12241 ttcaagtaaa aacctgatgt aaaatgtcta tgactacctt tttttagttc ttccatccac
    12301 attggattga acttccagtt ttctgggtct ctgtaaaatt catctattgc cattctgtat
    12361 gctcttacaa cagtagctac atagtatgca gtcttcattc taccttctat ttttaaactt
    12421 gttataccag atttgattaa ctctggtata tactctatca tacataaatc ttttgagtta
    12481 aagaagaatg tacccctttc atcttcgtat attggaaaat actcaccagg tcttttttct
    12541 tccaccaaat gatattgcca tctacaagat tgagcacaag aacctttgtt tgcatctctt
    12601 ccagtcatat agttgcttat taaacatctg ccagaataag aaatacacat agcaccatgc
    12661 ataaacgctt ctatatccat atctaaaggt gtttttgctc ttatctcaga tatttcatca
    12721 aaagataatt ctcttgccac tacaactctt tttattccat gctcgtacca gaagtttgca
    12781 cttagataat tggttgtatt tgcttgtgta cttaaatgta tttccatatt cggaattact
    12841 tttttaatga cactaagcac tcctgggtca gctattataa ctgcatctat tcctatctct
    12901 tctagttcca acaaatagtc ttctaattgt agaaaatctt cattatgagg tattatattt
    12961 actgttacga atactttctt gcctctttca tgtgcaaaag ctactccctc tgccatatct
    13021 tccttactaa aattcttcgc tgcagacctc attccaaaaa tttctccacc tatataaacg
    13081 gcatctgctc catatgttat agctattttc aatctctcca aatcaccagc tggagctaaa
    13141 agttctactt tatttaattc catctacatc acctctttta ctttctttat aacttagtgc
    13201 tacaccatca cctattggta ccaatgatgt gcttaaatag tcacaattac atatataatc
    13261 taaataattt ctcattcttt taactatagt cttttttctt cttacaacaa aatcatcatg
    13321 agcaaccata cctttataca aaatattatc agatactaaa agtcccccat cttttaattt
    13381 atctataact aaatcaaaaa atagcttata ttgacctttg gctgcatcta taaatataag
    13441 gtcaaattca ccttggactt gactaagttt ttcttctgca tctccttcta gtatagttat
    13501 gttattgtca aatccagcta attttatgtt ttcttttgct ttttctatca ttttttcatt
    13561 tctctcaaca gttattatat ctgcatcttt tcctataata gttgcaaaaa aaatagaaga
    13621 ataaccaatt gcacatccta cttctaaaat tctctttggt ttttgaacct ttaacaagac
    13681 ttttaataaa tctgaaactt ctttatgtat tataggaaca ctattttcct ctgcataaat
    13741 ttctaaatct tttaaaaatc cttctttttc ttttaatgtc actcttatat aatcttcaac
    13801 caaatcatta actatattac tcataaaaac actccctaaa ataataaata ttcaagtttt
    13861 atattaattt attatttttt aaattcatat tgttatatat atttacactt tctttattat
    13921 aaatctaaga ataacaattt tcaataaaaa agcccaacat tattcttacc ctgttagact
    13981 ttttataatc atggtttatt tttttgtatt ttctttattt gttgcttttg tatcttttga
    14041 ttgtttatcc cttgcttctt tatattcttt aacgtatttt aaatggtctt gatagtttgt
    14101 actataatta tttccaccat caatcttagc tacaaaatat aaatagtctg ttttctctgg
    14161 atataatgct gcttcaatag attttatacc aggatttgat attggtgtag gaggtaatcc
    14221 cttattttta taactattat atggtgaatc aatttttaaa tcatcatatg ttacaatttt
    14281 ctttctctct tcaaatatat attgtattgt agcatctgat tgaagaggca tacctacctt
    14341 aagtctatta taaaacacac ttgctattat aggtctgtct ttgtcaagaa cagcttcttt
    14401 ctcaattatt gatgccattt ccattacttc ttgaagagtc atatttaatt ctttttgttt
    14461 ctttttaaac ttgtccgtgt atttactgtc aaatactttt aacatttcgc ttaaaatatc
    14521 ttcttctgat tgtttctttg aattaaaata ataagtttct ggatacaaga acccttctaa
    14581 agaggttatt ccatcttcct tcaaatatgg aaatttatca taaaattctt gaggtttttt
    14641 tattaaattt tcaaaagtag ctttatctcc taaattttta cttactaaca ttgctataat
    14701 ttctttagat gtagctccct ctggaatagt cacttttata ccatcctgat atattttacc
    14761 tgaaactaaa agacttatta tatcattgtt tgaatacgat tgattaagta agtatgttcc
    14821 tgattttata gacggagctt tattgctaac ctttaccaaa agtttaaata atagttcatt
    14881 ctttattaat ttattttcat ataatatgtc agatattttt ccaacagatg ctccacttgg
    14941 aacgtcgata attacatctt ttttgttatt tttatcataa ggacctattt gtataaacac
    15001 aaatatacct gctaagataa tcaaaataac aattattaat actgctattt tcaatctatt
    15061 ttctttaaag ttcataatat aatctactcc ttatgtatat ttatccattt tctattataa
    15121 aactagatac tttaatttt
//
LOCUS       DAMKMI010000100        15116 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMKMI010000100 DAMKMI010000000
VERSION     DAMKMI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15116
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.104"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            80..250
                     /locus_tag="PC429_002677"
     CDS             80..250
                     /locus_tag="PC429_002677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848174.1"
                     /translation="MNNDKPFIEFEEEVDFILTECKKEGVSIDRDTIELILDLDLKFL
                     ELKGIATPLEDE"
     gene            complement(309..3290)
                     /locus_tag="PC429_002678"
     CDS             complement(309..3290)
                     /locus_tag="PC429_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDF3848175.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVDDLFRFL
                     ENTQKELLDEFKYNRGSNYKKAFIDLLNSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKKDRNPKETLFGFNQRVLVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     RGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKGNQRAIFPRFHQLDAV
                     RKIVEDLKINKVGKNYLIQHSAGSGKTNTIAWTSHRLSSLHDEENNAIFDSIIVVTDR
                     KVLDKQLQNAIYQLEHKNGLVVKIDEDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLDKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKVIDAENEKNEKSSIDKMN
                     ELIEKRTNLGNISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPISVYYKVGKKIIDNPEFDKGEAKRAINKFVSLNEHNIRQKVETIVDDFVDNR
                     SMWLEGKAKGMIVTASRLHAVRYKLAIDQYIKEKGYDMKALVAFSGTVKDGDDEYTEV
                     GMNKEVAPDITDSNLPNIFDKNEFKLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKQTFILDFQNSVEDIQDAYKPYFEMTNIDKVTDPNVVNDLWYQLHEY
                     AVYTDEEVNDFTVLFYKEKRTSSQDAKMNNLIDKAVERYCYLEEDDEKKSDEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLKKLPKKSKQRIDLDDMLSLDYYKLQKL
                     GLDDKKGTDISLYNGEGELVGISETATSRVSEADEEYLEDIIKKINDVFGVGLTEDDR
                     IMINTYEEMFKNDKDIMDIAKANSYEDLVSTFGKKYFKRGIVKTKNRNDRLVKEILTN
                     NNLQNYMIHYLAEKIYEEANR"
     gene            complement(3325..4596)
                     /locus_tag="PC429_002679"
     CDS             complement(3325..4596)
                     /locus_tag="PC429_002679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848176.1"
                     /translation="MDKNIFQININTDVLIRLRNQINEEYYISYRKEFYDKNNKKHRA
                     WDKICAIMDRLDDTVDYLNKLKLNTGKYRRSAFDFFEFMNQASVVMDCITHLAKIFEV
                     PYEEVETSTEIFNELGKEGKGTDKKYFEYLRSLCSVHPMETSRHKIYQENSFECSPYV
                     VWNDKSIWTNDASDLYAVVYTSKDNDSFKRIHIYMNQIFEYVKRRVNFIENIIEHIEK
                     YQQEIIEIFRNTPIKKEEDFESYIDYLRNLKKEIEIRYGNDSFHELNYLIELFDLPLS
                     NPDNEEKMNLYKNALKYAIKFEHNSIQNMTNEGYKNNGILYPQVNITTTLLEELISLN
                     SGSEERRKYRYAIEKTSYLSYDSCWSDREWAYDQIDDMMPFLERYVTFDKAQSDFEHY
                     VLVKLALYLECLENECLINKNVPNDKKFRIK"
     gene            complement(4658..6049)
                     /locus_tag="PC429_002680"
     CDS             complement(4658..6049)
                     /locus_tag="PC429_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF3848177.1"
                     /translation="MGYRYRSDDELKDSGVEWLGKIPKDWEVRQLKRVLSDRKENNEP
                     IKTSNILSLTIEKGVIPYSEKGTGGNKAKEDLTQYKLVYPNDIVLNSMNVIVGAVGLS
                     KYFGCASPAYYMLYKINANDDIRFYNYIFKSKLFQDSLKGLGNGIMMKQSETSGKLNT
                     IRMRIPMEKLSSVKIPIAFNLDQEKIANFLDEKTSQFDSIISKKEKLIERLEEAKKSL
                     ISEVVTGKVKVVKTDDGYELIKRSSEEMKDSGVEWLGEIPKEWDIKRLRFLGKLQNGI
                     SKSSDSFGYGYPFVSYSNVYRNIELPHIVEGLVNSTEEERKIYSVEEGDVFFTRTSET
                     VEEIGFASTCMKTIDNSTFAGFLIRFRPNDKRLYKGFSKYYFRCDLNRKYFVKEMNLV
                     TRASLSQNLLNNLSVVLPKYEEQVEIYNALELKVSYINLSINKLKYQIEKLKEAKQSL
                     ISEAVTGKIEILD"
     gene            complement(6049..8076)
                     /locus_tag="PC429_002681"
     CDS             complement(6049..8076)
                     /locus_tag="PC429_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF3848178.1"
                     /translation="MSIDNNFNHVAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIIKSVYEEYDFLPEEERDELVIVDLKENHDINLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEINQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNKMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEILFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKINPSAIVNVYGQELLDESFAICQADMLMKGQNPDNIRLGNT
                     LTQDRFNGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEAKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEQIKEI
                     YEKFEESENSKIFDNEDFGYRKVTIERPLKLSFKVNEEAIENVKNTTQFINLAVSKKK
                     DIEVKEKEEAEGKIKQDKLIKLLESFDNTLEYMDRDKFIKDLKAKSKEFDIQLSAGLI
                     KAIVNTIGVRNEDAEVCKDSKGNIESDSSLKDTESIGLKDDVYEYFEKEVKPHVNDAY
                     IDESSINNIGYEIPFTRHFYKYEELRSFAEIMKEVESLESEIALKIRKVLG"
     gene            complement(8191..8540)
                     /gene="ssrA"
                     /locus_tag="PC429_002682"
     tmRNA           complement(8191..8540)
                     /gene="ssrA"
                     /locus_tag="PC429_002682"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8698..9153)
                     /gene="smpB"
                     /locus_tag="PC429_002683"
     CDS             complement(8698..9153)
                     /gene="smpB"
                     /locus_tag="PC429_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF3848179.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            9759..10586
                     /locus_tag="PC429_002684"
     CDS             9759..10586
                     /locus_tag="PC429_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF3848180.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            11586..12049
                     /locus_tag="PC429_002685"
                     /pseudo
     CDS             11586..12049
                     /locus_tag="PC429_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(12316..12960)
                     /locus_tag="PC429_002686"
     CDS             complement(12316..12960)
                     /locus_tag="PC429_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848181.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(12890..14179)
                     /locus_tag="PC429_002687"
     CDS             complement(12890..14179)
                     /locus_tag="PC429_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848182.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(14176..15048)
                     /locus_tag="PC429_002688"
     CDS             complement(14176..15048)
                     /locus_tag="PC429_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848183.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         4885 a         2490 c         1654 g         6087 t
ORIGIN      
        1 ttacaaataa tgttattttt ttctcttctt tgatataatt taaagtaaat acattatatt
       61 taatcaaagg agcaaaaata tgaataacga taaaccattt atagagtttg aagaagaagt
      121 tgattttata cttactgagt gtaaaaaaga aggtgtttct atagatagag atactataga
      181 gcttattctt gatttagact taaagttttt agaattaaaa ggaattgcta cacctttaga
      241 agatgaataa tatacaatag ctcataataa agtacctatg gagagttaat tagctctcta
      301 taggtatctt atctatttgc ctcttcatat atcttttcag ctaaataatg aatcatataa
      361 ttttgtaaat tattattagt taatatctcc ttaactaatc tatcatttct atttttggtt
      421 ttaactatac ctctcttaaa atacttctta ccaaaagtgc taactaaatc ttcataacta
      481 ttagctttag ctatatccat gatatccttg tcattcttaa acatctcttc ataagtattt
      541 atcataattc tatcatcttc cgtaagtcct acaccaaaaa catcatttat ttttttaatt
      601 atatcctcta aatactcttc atcagcttca cttacacgag aagtcgcagt ttcagatata
      661 ccaactaact caccttctcc attatataag gatatatccg ttcctttttt atcatctaat
      721 ccaagctttt gcaatttata ataatcaagg cttaacatat catcaaggtc tattctttgt
      781 ttactctttt taggtaactt ctttaaaagt gctactaaat aaacttgcaa tcttaaaaga
      841 tttaagttct ttataggata aatctgaatt aaaaaattat aaaaagtaat atacttttga
      901 cattttttct taaactcatc agattttttt tcatcatctt cttctaaata acaatacctt
      961 tcaactgcct tatcaattaa attattcatc ttagcatctt gagaagaagt tcttttctct
     1021 ttatagaata atactgtaaa atcatttact tcctcatcag tatatactgc atattcatgt
     1081 aactgatacc aaaggtcatt tactacattt ggatctgtta ccttatcaat attagtcatt
     1141 tcaaaatatg gcttatatgc atcttgtatg tcttccacac tattttggaa atctaatata
     1201 aatgtttgtt tattaggata agttctatta agccttgata aagtctgaac agttttaaca
     1261 ccatctagtt ttttatccac atacatagca caaagttttg gttggtcaaa tccagtttga
     1321 tacttttctg caactattaa tagcttaaat tcatttttat caaatatatt aggtaaattt
     1381 gaatcagtaa tatctggtgc tacttcctta ttcataccta cctctgtata ctcatcatca
     1441 ccatctttta cagtaccaga aaaagcaaca agtgctttca tatcatatcc tttttctttt
     1501 atatattggt ctatagctag tttatatcta acagcatgta atctacttgc agtaactatc
     1561 atacctttag ctttaccttc taaccacata gacctattat ctacaaaatc atctactata
     1621 gtttcaacct tttgccttat attgtgctca tttaaactaa caaacttatt tatagccctc
     1681 ttagcctctc ccttatcaaa ctcagggtta tcaataattt tctttcctac tttgtaataa
     1741 acacttatag gcataaagtt ttttagtaca tctaatataa atccttcctc tatcgcttgt
     1801 ttcatagaat ataaatgaaa ttcatgaggt agtccatctt cacctatcct cccaaatact
     1861 tgcatagtct tattcttagg agtagctgta aaagcaaaga aacttatatt tcctaaattt
     1921 gttcttttct ctataagctc attcatctta tctatactag acttttcatt cttttcattt
     1981 tccgcatcaa ttaccttagc ttcttctaaa gtctttcctg ctaaagtttc ttttaatgca
     2041 ttcatatttt caccagatgt tgaagaatgt gcttcatcta taattactgc ataacgcctt
     2101 ttatcaagtt gttctatctt aggtagtgca tactggaatt tttgaatagt agttataata
     2161 atctttgctc tatttacaat agcatctgct aagtctgatg aatttttatc ttcatctatc
     2221 ttaacaacaa gtccgttttt gtgctctaat tgatatatag cattttgtaa ttgtttatca
     2281 agtacctttc tgtctgtaac tacaattata gagtcaaata tagcattatt ttcttcatca
     2341 tgaagacttg ataacctatg tgaagtccat gctatagtgt tagtcttacc actaccagca
     2401 ctatgttgga taagataatt tttacctact ttatttatct ttaaatcttc tactatcttc
     2461 cttactgcat ctaattgatg aaatcttgga aatattgctc tttgattacc tttatcatct
     2521 tcttgaataa agtaaaatct ttttataata tcaagtaaac tatctttagt aagtatttct
     2581 tcccaaagat aagaagtttt ttcttttcct tctactacag gatttccctt acctctattg
     2641 ttacctttat taaacggtaa gaagaatgtt ttatctcctt ttagctctgt tgtcatataa
     2701 acttcatcag tatctactgc aaaatatact aacactcttt gattaaatcc aaataaagtt
     2761 tcttttgggt ttctatcttt tttgaattgc tttatagcat tatacacatt ttgattagta
     2821 aactgatttt taagttccat tactataacg ggaaatccat ttaaagataa tactatatct
     2881 aaagtattgt tgtgctttga gctatatttt aattgtcttg ttattttgaa tatattttgt
     2941 ttataatttt catacgctgt tttattagta gttagatttg gtttgttgta gaataattga
     3001 aattttccgt tcatcattat atcttctaca ccatttctaa gtacacttat aagacctctt
     3061 cttttgatgt ttgaatttaa taaatctata aaagcttttt tgtaattact tcccctgttg
     3121 tatttaaact catctagtaa ttctttttga gtattttcta agaatctaaa taaatcatct
     3181 acaaataagc aattttcaag gtcaaattct tgtgaatgac cttgttcata tccaattgaa
     3241 actaaatgat tttcaataga ttcttctagg tgtttttctt ttggtaacat aatatcctcc
     3301 ctctccctat tttattagct ataattattt aatacgaaac tttttatcat taggaacgtt
     3361 cttatttatt aaacattcat tttctaagca ctctaaataa agtgctagtt ttaccaatac
     3421 ataatgctca aaatcacttt gagctttatc aaatgttacg tatctttcta aaaaaggcat
     3481 catatcatct atttgatcat atgcccattc tctatcactc caacaactat catagcttag
     3541 atatgatgtt ttttctattg catatctgta cttacgtctc tcctcactac cactatttaa
     3601 tgaaattagc tcttctagta atgtagttgt tatattcact tgtggatata atattccatt
     3661 attcttataa ccttcatttg tcatattttg aatactatta tgctcaaatt tgatagcata
     3721 ttttaaagca tttttatata agttcatctt ctcttcatta tcaggatttg ataatggcaa
     3781 atcaaataat tctataaggt agtttaattc atgaaaagaa tcattaccat atcttatttc
     3841 tatctctttt tttagatttc ttaaataatc tatataactt tcaaaatctt cttctttctt
     3901 tattggagta ttcctaaaaa tttcaattat ttcttgttga tacttttcta tatgttctat
     3961 tatattttca ataaaattta ctcttctttt tacatactca aatatttgat tcatatatat
     4021 atgaattctt ttgaaactat cattatcttt acttgtgtat accactgcat ataaatcgct
     4081 agcatcatta gtccatatac ttttatcatt ccaaactaca taaggactac attcaaaact
     4141 attttcttgg tatattttgt gtctactagt ttccattgga tggactgaac acaatgaacg
     4201 tagatattca aagtattttt tatctgtacc tttaccctct tttccaagct cattaaaaat
     4261 ttcagttgaa gtttcaactt cttcatatgg tacttcaaat atttttgcaa gatgtgttat
     4321 acaatccatt actacggaag cctgattcat aaattcaaag aagtcaaatg cacttcgtct
     4381 atatttacct gtatttaatt tgagtttatt taaataatct acagtatcgt caagcctatc
     4441 cattattgca catattttat cccaagcgcg atgtttttta ttatttttat cataaaattc
     4501 ttttctataa gatatataat attcttcatt tatttgattc cttaatctta tcagtacatc
     4561 tgtatttata ttaatttgaa atatattttt atccattaat tctccctttt atttaacttt
     4621 gtatgtataa tctatatcta tattaagata ctagaagtta atctagtatc tctatttttc
     4681 ctgttactgc ttcacttatt aaactttgtt tagcttcttt tagtttttct atttggtact
     4741 ttaacttatt aatcgataaa ttaatgtagc taacttttaa ttcaagtgca ttatatatct
     4801 caacttgttc ttcatactta ggcaatacaa cagaaagatt atttaaaaga ttttgagata
     4861 aagatgctct agttactaaa ttcatctctt ttacaaaata ttttctattt aaatcacatc
     4921 taaagtaata tttagaaaat cctttgtata gtcttttatc attcggtcta aaccttatta
     4981 aaaatcctgc aaaagtagaa ttatcaatag ttttcataca cgtagaagca aaaccaattt
     5041 cttctactgt ttctgaagtt cttgtaaaga atacatctcc ttcttctact gaataaattt
     5101 tacgttcttc ttcagttgaa tttactagac cttctactat atgtggtaat tctatatttc
     5161 tatatacatt actataacta acaaatggat aaccataacc gaatgaatca ctacttttac
     5221 ttataccgtt ttgtagtttt cctaaaaatc taagtctttt tatatcccat tccttaggta
     5281 tctcacctaa ccactcaact ccactatcct tcatctcttc actacttctc tttataagct
     5341 catacccatc atcagtctta acaaccttaa ccttaccagt aacaacctca cttataaggc
     5401 ttttctttgc ttcttctaat ctttctatta acttttcttt cttagatata attgaatcaa
     5461 actgtgaagt cttttcatca agaaagtttg ctattttttc ttgatctaag ttaaaagcaa
     5521 ttggtatctt tactgaactt agtttttcca ttggaattct cattctgatc gtatttaact
     5581 tacctgaagt ttctgattgt ttcatcatta ttccattacc taatcctttt aaactatcct
     5641 ggaataattt agatttaaaa atataattat aaaatcttat atcatcgtta gcatttattt
     5701 tatataacat ataatatgca ggactcgcac acccaaaata ttttgatagt cctactgcac
     5761 caacaattac attcatacta tttaatacaa tatcatttgg atatactaat ttgtattgtg
     5821 ttaaatcttc tttagcttta ttaccaccag tacctttttc agaatatgga ataactccct
     5881 tttctattgt caatgacagt atattacttg tttttatagg ttcattattt tcttttctat
     5941 ctgatagtac cctttttaat tgtctgactt cccaatcctt aggaatcttc cctaaccact
     6001 caactccact atcctttaac tcatcatcac ttctatatct gtatcccatt accctagcac
     6061 cttccttatc tttagtgcta tctcactttc taaactttct acttccttca taatctcagc
     6121 aaaacttctt aactcttcat acttataaaa atgtctagta aatggtatct catatcctat
     6181 attatttata ctactttcat ctatataagc atcattaaca tgaggcttta cttccttttc
     6241 aaaatactca tatacatcat ctttaagacc tatactttca gtatctttta atgaactatc
     6301 actttctata ttacctttgc tatctttaca aacttctgca tcttcatttc ttacaccaat
     6361 agtattaact atagccttta taagtcctgc tgaaagttgt atatcaaact cttttgattt
     6421 agcttttaaa tcttttataa atttatccct gtccatatac tctaaagtat tgtcaaagct
     6481 ttctaaaagt tttattaact tatcttgttt tatttttcct tcagcttctt ctttttcttt
     6541 aacttctata tcttttttct tagaaactgc aagatttata aattgagtag tattttttac
     6601 attttctata gcttcttcgt ttactttaaa gcttagtttt aaaggtcttt ctattgttac
     6661 ttttctatat ccaaaatctt cgttatcaaa tattttacta ttttcacttt cttcaaattt
     6721 ttcatatatt tcttttattt gttctatttg ttcaagactt atttcttttc ttttgtttcc
     6781 taagcttttt ctcattaatt gataatattc actagcattt acaagttgta tcttaccttt
     6841 tcttttagct tcttttttgt tagtaagtac ccaaatatat gtagctattc ctgtattgta
     6901 gaacatatca gtaggtagtg ctattatacc ttctagtaaa tcattttcta ttatccattt
     6961 tctaatgttt gattctccac ttcctgcatc tcctgtaaat aatggacttc cgttaaatat
     7021 tatagctatt ctacttcctt cttcatcatc atacatttta cttatcatat tttgtaggaa
     7081 aagtaatgaa ccatcactta ctcttggagt acctgctcca aatcttccat caaaacctaa
     7141 gtctgcttct tcttttactt tcttttcttc gtctttccaa gttacaccaa aaggcggatt
     7201 tgatattaaa aatcttattt tttctccgtt aaatctatct tgtgttaaag tatttccaag
     7261 tcttatgtta tctggatttt gccctttcat tagcatatct gcctgacata ttgcgaatga
     7321 ttcatcaagt agttcttgtc catatacatt tactattgca cttggattta ttttttctac
     7381 atagtcttca gctattgaaa gcattcctcc agtaccacaa caaaagtctg ctatagatat
     7441 tacttttcct tcttctgtaa ggaagttttc tttacccata aatagtattt ccatacaaag
     7501 tcttataact tctcttggtg tgtattgctc tcctgctgct gagttttctg taaatcttct
     7561 taacatttct tcgtatatgt atcccatttt attgttagat actgagttta tatgtagatc
     7621 tacttcatac attttgctaa gaactgcata tagcttgtct tttttatcta attgatttat
     7681 ttcatcttta aatttaaatt tacctattat ttcttttaca ttgtttgaga atccgtttag
     7741 atattcttca aagtttgatt ttatattttc actatcatct aatagttttt tccatgtaaa
     7801 atcacttaca ttgtaaaatt gtaagtttat gtcgtgattt tcttttaagt ctactattac
     7861 tagctcatct ctttcttctt ctggtaagaa gtcgtattct tcgtatactg attttattat
     7921 ttctctatca taatcatcta atagacaatc aaatcttctt attactatta gtggtaacat
     7981 tacttttcta tagtcttctt ctttatatgt acctcttaaa ctttctgcta tattccacag
     8041 aaatgcaaca tggttaaaat tattatctat actcacttat actatctcct tttagttaaa
     8101 ttttacatta gtttatatta taattatatc atttcaaggc acaaaaaaaa catattgact
     8161 aaactatcaa tatgttttcg tggatttcaa tggtggagac gaggggagtc gaacccctgt
     8221 ccgcaaatct tttccttgct gcatctacat gcttagccaa cttcactaat atctcacctc
     8281 acatagcgcc aagtggcagg ctctatgctt agccagtccc aattaatcta cttcaagtcg
     8341 agacacccct aaagtagttc cctgcataat gacgtccagt cttagtcggc aggaggacta
     8401 agagggacga gcagcaacta ggctgctatc gcgaaattat cgtctgcgtt tattttaaag
     8461 ttccccagtt tttacgtgac ccagagtaac acgacatgca gcctcaagtt ccaaacccac
     8521 gtcgaaacct ttacgccccc ttgagtagtt tatagtccag tagacttttc tcaaacttta
     8581 tcaccatttt tattaaattt ttaatgtata tataatataa tacttttaag aataaaaatc
     8641 aatgttatgt tctagataat aaagaatttt attctaaaaa atacagtttt atctagttta
     8701 atacttacct gtcatttctc tttctatacg tctttgtgca tctttcttag ccatatcttg
     8761 acgtttatca tatagttttt taccttttgc taaggcaatc tctactttaa ctttaccgtt
     8821 ttttagatac aaactaagtg gtataagtga ataccccttt atagtagttg ctccaataag
     8881 ctttcttatt tcatgcttat gaagtaatag ttttctaact cttaaagaat caacattgaa
     8941 tatatttcct tgctcatatg gacttatatg aacttgtttt aaaaatactt cgctattatc
     9001 tacagatgca aatccatcac ttaggttaac tttgccttgt cttattgatt ttacttctgt
     9061 tccttttaat tcaattccac attcataagt ttcttctata aaatattcat gtcttgctct
     9121 tttgtttgtt gctaatattt ttttaccagc catataaatc tcaccttctt tgctagaaat
     9181 ttgttccata aatgttagaa atttactaaa tatttaatgt tgccaaatta tttgactaac
     9241 ttgtatgata acatatttag gcattttagt caatatttca ctgatatatt aagttagtta
     9301 ggctatatat aattaagtaa tataaatgta tgaaaatagc taaaaaacta cttcgaatat
     9361 acttaatact aaactaactc taaccagtaa tactaattta taaatttcaa aacaaaaata
     9421 ttacatatta taaatcttct atcttctatc ttctatcttc tatcttctat cttctatctt
     9481 ctatcttcta tcttctatct tctatcttct atcttctaaa taattttaac atgcccataa
     9541 aatataatac ccacaaatga taaataatac tcaaaatgtc tattgaaata actatacatc
     9601 cttaaataac ttaacttaaa tctaaacatt tcaaaaactt cttaaaaata tgtaaaaatt
     9661 tgatgataaa gtagcgattt tttcaaaaaa aatgtaatat agagttatat cttataatat
     9721 tttagagata aattttatca tagtggggag aaatttgtat ggatttaagc aaaaaagtaa
     9781 aaattttaga aaatgcatca gatggcaaaa gcgaatttga gatattagaa tttgaaaaac
     9841 ttgagggagc ttctagccca ttaatggcaa tgggattata ttttgctaga gaagctggtt
     9901 tgaaagctag acaaattaag ataaaattaa ataattcatc aataaaaaca gaagctggtg
     9961 cattatacta ttataaagga aatatatctt ctgaaactaa gattggtggt gttggaggtc
    10021 tttttaaaaa aggaatatca ggagcactta ctaatgaatc aatgatgaaa cctgtataca
    10081 aaggtactgg agaagtttat ttggaacctt cttttagaca ttatttattt atgagtcttg
    10141 ataatgattc tataattgtt gataaaggat tattctattg ttgctctgat acaatagaag
    10201 taaaagcatt tacacaaaag aacttctcat ctgctttact tggtggtgaa ggcttcttcc
    10261 aaatagaatt aagtggtact ggtgtaatcg ttctggaatg tgttgtacct caaagtgaaa
    10321 ttgtagagta tgaattgaaa aatggtgaag aattaaaggt agatggtaac tttgcaatag
    10381 caagaacatc tggtgttaat ttttctgtta caaaatcaga taaatcttta attggttctg
    10441 ctttaaatgg tgaaggtttc ttaaatacat ttactggtag tggtacagtt tggctagctc
    10501 caactcaagt aatgtatgaa aaactaagtt ttggagcaat gcctactaat gataatatga
    10561 ataataaagt ttctaagcaa aaatagttgt tttataaata tttattaaat cattattgat
    10621 tttataaagt cactataaat tttattagat tattataaaa tcagagtgtg gaaatattta
    10681 tgtgtattct ttaaataaaa atttccacat tctgtaaagt tattatactt gatatataaa
    10741 attcaattta ggaacatagt ctaacattta taaacatatt aaggtaatga tttctcttgg
    10801 tagttattct catatcttat tctttgatta tatttatctt attcctagcc cttatgtcat
    10861 attatatttc ttatcttatt atttataatt agcattaact tatctaaaat cttgtatttt
    10921 tttaatagta aagtttacat ctatattaat ttcataaaac aatcaaggta gtcaagagat
    10981 tcaactttat ttgattttaa aaatttaagt gaatttgttc ttatacacac ctttttaaaa
    11041 ttatatctgt agaaaatatc atttagagct ataattaaat taacttatta aattttagaa
    11101 aatttattaa aaaatataag ttaaattaat atttttaaag attctttttt gtaactacta
    11161 gaacactaat ttagtagtta ttgtatctct aaagtttaag catggataga tatacatttg
    11221 ataatttgcc tataatttac ctactacttg gattattgga gcaaatacaa tatattaata
    11281 ataagttcct aaaaccaata aattaactaa tacacataaa gatattgatg ttgattatac
    11341 aactataaga aacggattta acagaattta atcaagctaa gaaattatat atatcgtgat
    11401 gtttttgaat tagaaggtga ttcaaaatta gtttatagtc ttctgtgaat ccaataaaaa
    11461 gtacagggta caagaattgt tagtaaaatt tttaaagatt ttatggataa atataaatag
    11521 ttttacttat tttctaaaac tttaaaatac cattaattac cttaaatatt ttaataggag
    11581 gacatatgaa tttaaataaa gttaaattta ctgaagaaca tgctattgaa gttactaatt
    11641 ggaaatatga aggtaaatat tcaatataca atctacctcc atgggaggaa attaaaaaga
    11701 agaatttttc actagcaaaa gaagataaaa gaaaaaattt tataagtttt ataaatgaca
    11761 ataaagaatt gataggtttt ataaacttat tagatgaagg aagctctgta ttctttggta
    11821 ttggttgaaa ccgaattatt gtggaatggg aattggtaaa gaaataatta gcctaggatt
    11881 acaagaatgt agaaataaat atagtactaa accaattgta ttaaatgtaa gaacctggaa
    11941 catgagagct gtaaaatgtt atgagtctca aggatttaaa atagttgaga ctaaggtaca
    12001 aaaaacgcat ttaggagatg gagaattttt tgttatgagg tatcaatagt ttataacaat
    12061 agtcctattg ttaatacaac aattaaaatg aaaaaataca tatgtgtagg tcatagcaat
    12121 aagtgtcttt gaaatataaa tttgagagct tttgataaaa ttcggtatac atatatattt
    12181 tatactacgg atatataaat ctatctacat acataaagag attgtaagta ggtttgtgta
    12241 tataaaaata gatttatatt tcataaatgt actttttatt tgcattacct ttattttatc
    12301 taagctctca ttttattatc taatcttcct ttttactggt tttattaaga gagatgcaat
    12361 tatcaacaca acacttccaa gtatcagcca atatttctca tcaaaactac cttgactcat
    12421 agacattata aaaaattttc caacatactt gcctttagtc atcatattta aaattaaata
    12481 tcctatagat atcatataac ctatgattgt attattaaat aaatttgaga aaaacattcc
    12541 taaacttcct ataaacatag ctgatgcgaa acttccaata atatactcaa ctactggaaa
    12601 attacatcca agatatatta aaacatttac aaaacctatg attaatacca ctatacctat
    12661 aattgacatc agtatcctaa ttaattgtac aataacttga gataatgctt ttgaagatac
    12721 tatctcatca atttcttttg attgctctag cgaaaataca ggtaccaaca ttactatccc
    12781 tattattgca acataatttt ctaaacatat agctgttgaa attctgtcta aattagctac
    12841 tccttgtatt agaggaatca tacatagcaa tataattgaa catattaaat tagttaaagt
    12901 gcttaatttg aagttttctt ttattagaac taaacagttt tgaaaacttt gcccaaacat
    12961 caagattccc ccttcttttt tgctcatata agaatatagc taacaaaaca agcaccaatg
    13021 ctcccaaact tatcacaatt ctattaatca taatttgatt gaagttttgt ataataatat
    13081 ccccatttaa tacactatta tgtcttatca ttagccccat tgttgaataa tcacctttga
    13141 gtcctgtaga atttatactg taaagccacc aaattaattg cacaataatt gctactggtg
    13201 tatctgttaa tatagtaata ctcattccta cagctgtaac tatcataaga gttggtagta
    13261 tccaccataa tgtatatttt ataaatgcta agttatcaat agatatacct ttatctttag
    13321 caaagaaaat caaaggaagt aaatctttca tacctaaaat taatacaggc aatatcatca
    13381 taaagacaag tgcaaaatat cttgataaaa ctattttgca tgaagatact tgttttgaat
    13441 aaataatctc tctcattctt gaacgcttat ctttaagtga cataaaaact gccacaaata
    13501 ctggaaatat acttaaaact atacctatat aatcgcaaaa aattcttgca tacccattac
    13561 taactttatc tttttgtatt gtatagttgt aattcttcat agcttcatca taggtaactg
    13621 gaactactcc aaatctttgt aacttgtcaa aagcatatgc agaccctcct cctaaaatgt
    13681 cgtctactct ttgcattatt tcttcaaatc tttcaagaga tactttagaa taatctattt
    13741 ttatactttc atcagttttc caaagtgaat ttatttcgct aatactttta ccagttattt
    13801 cttctatata tttttctatg gtcttcgttt tattttggtc taattttata tacttaacta
    13861 cccctgttgg atacgttgta tatgtgtttt tttgatattc tctaattagg tttatagttg
    13921 catttggaat gactttttct gttttcatag attctttttg accatatgga tattgattgt
    13981 gtacagagta atattcctca atattctcta ctttttgtgg agcaactaat ggatttccat
    14041 cattaggaat atttttactt acatcatttg aaactgaact tccaagttga gaaatataga
    14101 aaaaaactaa aactacaata aaaatataat atatcatact ctttaatata tttttacatt
    14161 cttttataaa tagattcata tattatctcc tcccattata agcatatagc catcttcaat
    14221 agttggatta caatttactg ctgtctcaaa aggtttatca tctgatataa tcctaagtat
    14281 agcctttcca ccatgagtca acattcctat aactataaat ctacttttta tattttcaat
    14341 atctttttta tctacttcaa tactatatac tttcccttca gctagttttg ctaatgcttc
    14401 tgttgagcca ttgtatatga cttttccact attcaatata gctacgtttt cacatgtagc
    14461 ttctatatct ccaacaatat gtgtagataa aataacgatt ctatcatttg caatttcgca
    14521 tagtaaattt ctaaatctta tacgttcttc tgggtcaaga cctgctgtcg gctcatctac
    14581 tattaaaact ctgggattat gaattaatgc ttgtgctatc ccaagccttc tattcatacc
    14641 tccagataaa gattttactt tcacattttg ataattagta aggtttactt tgcttaataa
    14701 ctttgctata atttttcttc tttttcttag tggtatatcc gataaaactg ctaaataatc
    14761 cattgcttta tatactgtca tgttaggata cattgaaaag tcttgtggca aataaccaac
    14821 catatttcta atttccttag tattctcaat aggaactcca cacatatgta ttgaaccttc
    14881 tgtttttgat aataaagtgg ctaaaattct cataaatgtg gtttttcctg ctccatttct
    14941 tcctaaaaga ccaaacatac cactgtcgat ttcaagattt atattatcaa gagcttgttt
    15001 tttcccaaat cttttactaa gattggatac tgctattttc atttccatat ataaatacac
    15061 ccccagaaac aattttatag aattttattt ttataaattt ttactgtatt ttcata
//
LOCUS       DAMKMI010000101        14884 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMKMI010000101 DAMKMI010000000
VERSION     DAMKMI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.105"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            207..602
                     /locus_tag="PC429_002689"
     CDS             207..602
                     /locus_tag="PC429_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848184.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            645..2744
                     /locus_tag="PC429_002690"
     CDS             645..2744
                     /locus_tag="PC429_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF3848185.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYNGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(2969..8179)
                     /locus_tag="PC429_002691"
     CDS             complement(2969..8179)
                     /locus_tag="PC429_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF3848186.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKEIM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWIISKREDSGSA
                     GAEGTAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8194..10689)
                     /locus_tag="PC429_002692"
     CDS             complement(8194..10689)
                     /locus_tag="PC429_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3848187.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(11372..11659)
                     /locus_tag="PC429_002693"
     CDS             complement(11372..11659)
                     /locus_tag="PC429_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848188.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(11679..11954)
                     /locus_tag="PC429_002694"
     CDS             complement(11679..11954)
                     /locus_tag="PC429_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848189.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(12591..12770)
                     /locus_tag="PC429_002695"
     CDS             complement(12591..12770)
                     /locus_tag="PC429_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848190.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(13055..14530)
                     /locus_tag="PC429_002696"
     CDS             complement(13055..14530)
                     /locus_tag="PC429_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3848191.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
BASE COUNT         4863 a         2617 c         1683 g         5721 t
ORIGIN      
        1 atatataaac tgcatatata agctatataa gaattatttc aactatataa actacatatg
       61 taaaattcat gtaaatttac taatataaat caaataaaat tactaaactc tctaaactaa
      121 ttttgtaaaa catactctat ttttaacatt tgttacctta tacataagta caaacaaaat
      181 ctaactaaat gaaaggataa tgtaaaatga atattttaaa aatgaaaaaa acagggatat
      241 ttttaggagg agtacttttt ggtactgtag gtgttaagat actttcaagt gattgtgtta
      301 aaaaaacttg cactcgtgca acagcaggag cattgaaagt taaagattct gttcttgaaa
      361 caactacgaa aattcaagaa aatgtagaag atattttagc tgaagcaaaa gaaatcaacg
      421 agcaagaaga cttatctaca tctaaggtaa acgttgtaca agatactctt aaatgcgaac
      481 aagaagttct taaaactgaa actataaaaa ctgtagaaga tactaattct aatagcatta
      541 aatgtgaaga aatcaaagat aacgtaattg aagaaaaaaa tagtacttgt acaacagaat
      601 agatttaatg attattatga ttaataatat caggaggaag taaattgaat ttttatataa
      661 aacacgaaat aagaggtcgt atacgctttg acttacaact tggcaaatta aacaacaagc
      721 agtccgatac tctcttatat tatttaactt ctataaatgg tattaccaaa gcaaaagttt
      781 acgatagaac tggtgatgca gtcatttatt acaatggtga aagagaatac ataataagag
      841 aaatcactaa attcagtttt aacaatagca aaatagaaaa cctagttcct gaacatacca
      901 gcagggaatt aaacaattat tatagagaca aattagcaaa taaactgcta gtcaaaagta
      961 tctccaaaat attttttcca aagtccttaa gatttttttt aattggactt cattctataa
     1021 aatattttaa aaaaggtctc tcttccctgc taaataaaaa aatagaagtt tctgttttag
     1081 atgccactgc tataggtatt tctattttta gaaaagatat caatacggct ggttcagtta
     1141 tgtttcttct tggtatcggt gaactattag aagaatggac tagaaaaaaa tctatagacg
     1201 acttggctca aagtatgtca ctaaacattg aaaaagtatg gttaagaaaa ggtgacactg
     1261 aaatcctaat tccaatttca gagataaagg aaggagattt agtaagtgta actatgggaa
     1321 atatgattcc attggatgga gttatagtat caggagaagc aatggtcaat caggcatcac
     1381 ttacaggtga atcccttgca gttaacaaaa aagaaggatc ttacatatat gctggaactg
     1441 taatagagca aggcaatatt gttatgtgcg tcaaggaaaa agctggtact actagattcc
     1501 agaaaatagt aactatgatt gaggaatctg aaaaactaaa atcttctgta gagagtaaat
     1561 ttgaacatct agcagataca ttagttccat acagcttttt aggaagtata ttaacctatg
     1621 caattactag aaaccctata aaatcactct ctatattaat ggtagatttc tcttgtgcat
     1681 taaaactctc tatccctatt tcagtactat cagccatgag agaatgtaac aataacaaca
     1741 ttactgtaaa aggtggaaaa tttcttgaag gtgttgcatg tgcagatact attgtatttg
     1801 ataagacagg cacattaaca aaagctcaac caactgtttc agatataatt acattccaaa
     1861 attataataa ggaggatatg ttaagacttg cagcttgctt agaagaacac ttcccacact
     1921 ccatagcaaa tgcagtagtt tatgaagctg aaaaacaagg gcttagtcac aaagaaatgc
     1981 atacagaagt agaatatatt attgctcatg gcatttcaac aaaagttgat gataaaaagg
     2041 ttgtaatagg tagcaaacat tttgtatttg aagatgagaa gtgtactatc ccagacggtg
     2101 aggaggataa gtacaataat ctatcagatg agtattcaca cctatttatg gcaatatcag
     2161 gtaaattatc agcagttatt tgcataaatg accctttacg aaaagaagca aaatatgtaa
     2221 tttcaaattt aagagaatgc ggaataaaaa agattgtgat gatgactgga gatagcgaaa
     2281 aaacagctaa atctatagca agtaaagttg gtgttgatga atattattca gaagtcttac
     2341 cagaagacaa agccaatttt gttaaaagtg aaaaactcaa aggtcgcaaa gtaattatga
     2401 ttggtgatgg aatcaatgat tctcctgcta tttcagaatc agatgtcgga attgcaatga
     2461 gtgaaggtgc tgaaatagca agagagattt cagatattac catatcagca gataatttga
     2521 ataatttaat cgttttaaag cagattagta ataagcttat gaaaagagtt gatttaagtt
     2581 ctaagtttat tatcggcttt aatttaggtt tgattttact tggtgtaggt ggatttgtta
     2641 gaccaagtac atctgccttt ttacacaacg catctactgt cggaataagt ttaaacagta
     2701 tgacaaattt attggaaaac accaatactt ataactacca ttaatctcca ttacataaaa
     2761 catatgatat aaaaaattta taattcatct ttttaaaaaa atatgtctta aaattagctt
     2821 ttttctaatc aaaattatat aaagcatact ttacaaattt taaactcaac ttatttctat
     2881 tttttacaaa aaaggagatg attcaaattt tcattttgaa acatcccctt ttgtaaattt
     2941 ctttaaaatt tatatattaa ttatattttc aaccaataat tgtattttca accaatcttt
     3001 cttcatagca ttttaaatca ttttgcttta gataattaaa ttcagactct acataaatac
     3061 cacagcctac caattcaatt gtcaaattag gtatattttc ctttagtata ctagtctgct
     3121 cataaataat tctttccaat aaatcaactt gtaatataga aaaatgcttc ataatctccc
     3181 catcactatc tatatctttc ttgatttcat accaactctt tgacaaaatt cccttaaaat
     3241 caatataccc tagactcttc aatatatcta tactatcgct ccatgcatac ttactaggtt
     3301 tatcaaagta tgactctagt ttcttcttct gctcatttgt aagtgaatca taagaaagct
     3361 tcttattaaa atgtgccttc aaagaaatct tagccataga atatggactt ctaccagagt
     3421 ttaatcctgc atttttgaac ttttcaattc ctttatcttc tactgaagac catattgagt
     3481 ttattgaatc tgaatcattg tatttaacat caagaccatg tgtaatattt atatagtttt
     3541 gtggaaatac atattgataa tagacatctt ttttatcatc atatagagag aagtatccac
     3601 tatcatataa atgaatatgc acctttactt ttaaactatc ctctaaaata tttcgtctaa
     3661 ggttcgggat atttgcatac atggttacat attttatggc atcaaagttt ttatttaatg
     3721 gaacttcaaa actaatttca tctcctgtca aaagcttttt cacttcttct tcattacaat
     3781 catatagaga atatgtattt tcaaatccca cacgtgattt cccatctaaa ctgcctgtta
     3841 atgctacctt caaaatatgg ttatctgttt tttgccattg gtaagcatct tggtcaagaa
     3901 actctctaat ctgtctattt tgctctttcg cctgatttat aacctcgttt tcatcttctg
     3961 taatccctac atggcatcta tttaagatat ttactatatt gcttccaaat gggaatgtcc
     4021 atagttgaaa agctgctgcc tcaacctgac acaagtatct aacctgattt aattcaattt
     4081 gatggataag ttttttctga tattcattca tctgattacc actatctgac atttgataaa
     4141 tcaattctcc cattgcaaga ttatcttttt tcttatcaat cttatcagta atgataattt
     4201 gagtttccat catatcctgt acttttttaa atattataga aatctcctct gaattttcca
     4261 tttttaagct tccaaacttt gtgcttaatt cagttaaaac ttccttcatc tttaatttac
     4321 ttatctcatt ttcaaaaata tcttgatttt taactgtact atcgaatttt tctacaaatg
     4381 gtataacaga ttctttcaaa gttttatctt taaattccac catttgagta ttcaattcat
     4441 ctaatttctt aatattattt tctttatctt taattgcctc tttaactacc tctattttac
     4501 tctcaaaact agattttaca ttaagacaat tattaacacc actaatcgca gtatttataa
     4561 gagaacaagc agaacttcca atactcaatg catttttaat tttagatgtc ttttgctgtc
     4621 tttcaatagc ttgtttactt ttctctagtg cagctaactt atctttttgc tgtttattat
     4681 tgtaatttgc ctccaacata cctatttctt ttttctggat ttttgtgtaa atttccttat
     4741 actcgctttc acttaacccc tcttttgctc tttgaatatc gtcagctttg tctgcataat
     4801 tatcatcatc tatagactta actataatct ccttaatttg gtcaaattta gttcttcttt
     4861 cttcatcaac acctgccaaa ttataagtca ttttatctaa agcatttttt ctatcttcac
     4921 ttagctgttc tccaagtaca aatctcatac catcagaatc caatttaaaa tctgaaaaat
     4981 taaccatcct gttagtccat ttgtattcct cttctttagc tgaaagattt tccttcaaag
     5041 attttgcact gtcaaatgcc cctagacaag cttgaccaac accactaaat gcagtcagta
     5101 aactcaatgc gctttgtatt ccagaaagaa ttaactgtgt tttcatttgt tcctcaagtt
     5161 ttgcctgttt cttttttagt tcctctatgt cttcttgttc atcttctttc atctttataa
     5221 tttcttgaat caacaggttc atttttgcat taacagaaag cattttattc tcaagttcat
     5281 tttttaaaac ctctatttgt tgatttccag attcaatttg agttaggata ctttccttat
     5341 atgtattgaa atatccttct tttaactcat ctttcttccc ttgttccaat tctcttaagg
     5401 tcttatccag ataatctgtc aaagaacgaa tatctgtaac acgatttcct ccaatttcac
     5461 tttcttcaat catctgcaca taaaatgctg tcttctgctc aagtgcaaat aaaatcatac
     5521 tccattcgct aaaattctga ttttttccta caaaagattc tattctatca gactgcatca
     5581 atttatttat aaggtattca tagatttcct ttgcttcttt ataaaattct ttctttgtat
     5641 tcatatcatc cagagaaagt actgctttat ttttcttctc aaaactatag tacttctgaa
     5701 gtatttctct atttaattgc atctctattt tctcaactgc atgaaaaatt tcataaatat
     5761 gtgaaagttc taaaatatca gcttctaaaa ttcctactgc acacaacagt ctaagatttc
     5821 tataaccttt aattccagaa aaaccaaatc tctcgaaaaa ttcatagaag aatgtatcta
     5881 ttgacaaaga atactcctgc tttaacaatt ccttttcttt tagtgcatct ccagagtgac
     5941 cggctgttcc ctctgctccg gctgaacctg aatcttcacg tttgctaatt atccagtcct
     6001 tatgttcttc ataaagagca tttaatttct ctccatcatc tgctgcttgc ttctgattta
     6061 aagcatgatt aaacacatat ctatcaaaat aatagtagtt tctatcacta ttatccattg
     6121 gaggagaggt acctatatat cttattgttt cttcatatat acctctaatt ccaccaagac
     6181 ctcctgcacc accagctcct ccagagcctc cttttcctgc taaaccatga tttccatctt
     6241 ttttacttat agcacttttt aataaaggag cttttccaag gagataaacc tgtccagact
     6301 tgcttccaag acctgcattt ccaccattac ctcctgtgcc tccagttcca ccttgttttc
     6361 ctttttctgc ttttaccaca tagtattttg tacctttaac attagcaaat tcttttgact
     6421 cttttttaga acctatttta gcatttgccc cagcttctcc agttatccct gtatctcctt
     6481 tttttccatc ttgtccattt ccaccacgtc caccatctac gtaaagttta agttctcctc
     6541 ttttttctat ttctttaaaa gataatatga tatcacctga agtacctgca ttattgccat
     6601 ttcttccatt cattccgtcg taaatagaac tttctccaac caatttttct gcatccttac
     6661 cagatacgtt gattgaataa gtcccttctg gtatatgtaa tttttctgta agtagtacaa
     6721 tattcttacc tgagaagact tctttctcca tattacagtc aattttcaac tcacaatctc
     6781 catacatata tatagatgac aaaatattaa gttgataacc agatattatt ttagtcaaat
     6841 ccgataaatt cactttaggt ataggaatat atatagaaag tacattttct ttctcatatg
     6901 attttatata tgtacaactt tgaatcttct ctatcctata ctctgcatct tcaagctttt
     6961 tggcttcttc ttccctatat tcttcttctg ctatccaata tttgatatct tttaatgtat
     7021 cttctaaagt attttttagc aatgatttta acaactcact tagaccaaca atatgagtct
     7081 cactacagta actatttaac tctaaaagaa gacgtgtttc ttctaatata ttcactttct
     7141 gtacgccatc accatatgaa agttcttgta caaactccat aatctctttc atattattag
     7201 tcctactaca taaatctgta tacatcttat ctaattgaat ctttttatct tttagactat
     7261 ttatagaaga tagaatggaa atagaatctt ccataccttt tttaacactt tctatataag
     7321 gaactaaaag tttttctagg tctttatagg ttacatcaac agctttttgt atttctattt
     7381 tacaagaagc agtaagcatc aaccccaaat ctgttttttc aaatgtttca ggtagatttt
     7441 tcatttcatc caacaacttt tctggctgtt tatctagtgg attacataat ttaatagata
     7501 caccatctga taatgctaca tttaataagt acactaattc cttacaaccg tcgtaatctt
     7561 tctcttgact atctaggtct tcttttgctt tttccaatat atttcttatt ttttcttctg
     7621 ttacattacc aattagtgtt ttaggtattt tattgataag aaagaagaaa ttttctttat
     7681 actttgcata atcaccaagc atatctttca aatttttgcc tgtttctatc aataatgtat
     7741 gtctagagtc cagtagggat tgatagtcta aaataactac catccctttt acctttgaag
     7801 atgtggaaat agtttccctt acaagcatag caccagcaat catatactct gctccacggt
     7861 tatcaaaaaa acctgggcaa tcacataatg aaaaatctct atttgcaata tctacaactt
     7921 ctggacataa agtcttagaa tatacaccat ttccaatttc gacagctgaa tcttttgcag
     7981 ttatatatat ttgtccagtc tcttcatccg ttttttcttc catcacacag ccttttaaat
     8041 aatttataca agtactcttt ccaacgcctg aagcacctaa gaataacaaa attgactttt
     8101 cttttaagtc atattttcta agctcgtctc ttccttcttc caaaaggttt acgatttcac
     8161 tataaagttt atttgccata catccatccc tttctatatt tcggaacaat agccttgata
     8221 tttttcttta ataagattct cttctacctt tgaaacagct gttgataact tagtgtttgg
     8281 agcattaaat cctccattgt ctcctctatc tccatattca gcgtattctc ttgctggtcc
     8341 tgttttacct tcattccagc ttccaccact aaattctata tgatatttaa ttatactatc
     8401 taacttataa tcccaacttg taaaatatcc tgtatagtat acagtcttat ctataccact
     8461 tggagaaaat gaaagtgtat tatatccgtg atagccacca cttcctccat atccacctaa
     8521 acctggtcta ccatttggac ctgcttctcc atctttcact gttatatcac aatgattaat
     8581 tattctattt ttttgttcct ctgaatctga aatgaagtaa atattacctt tcttaccagc
     8641 atttccacct cttccagcat ttccaccagc tccacctttt tcaccttttt tgccacttgt
     8701 acctttttca aaataccctt taaacatctg tccgtggaaa taacttgtat atcctgcatc
     8761 agctccatta cttccagaag ctcctgtttc accattttcc ccatctattc ctttagaacc
     8821 atttcctcca tttgcaatca gtgttagacg ataatttcta atttcttctt taatatataa
     8881 gtaaatatta cctgcatctc taccatcttc accatttttc ccatacatag aattatctac
     8941 aagttctccg ctctttccac ttacattaat ttttacatta ctacctacat caagattgtt
     9001 agaagcaaga ataatatttt tctctgatgc aaagttcatt ccaaggttat catccatata
     9061 aatagtatct ttagcaaaga acataaatgt agaaacattg tcatctactt ttctattttt
     9121 gaaatattcc aatacactgc taattactaa gtttggaatt aatgctctta tttcaatacc
     9181 tttcttatca ttaatattaa ttgtttttac tataaatgga tttaaaatgt atttaccaat
     9241 aaattctcta attatctcat taccttcttc tccatatgca cttaatcttg ttatactgtc
     9301 ttctaactct tcattttgtg taactgtaac atttgtcaaa attgaattaa aatcacttac
     9361 actatataca taatcctttt tatttgcaac ttccattaat ttttctctat ttggtaatgt
     9421 aaatacctgt ttttgcactt cataagaaac taaaactttt tctaaattct ttcttattat
     9481 aatgaagttt tgttccatta tcttcttact tgccttttct ataataaatt ccatatgtac
     9541 agaaggtaat aaaatttccc cagttccatg ttctgtatat ttacttaaag catcaattat
     9601 atctaagttt ccttttatct tattttgtaa tttcatgcaa ctatcttgta agaataatcc
     9661 actttgtttg gctaatacgt tgataaatct ctctatagta agacttgttg tactttccaa
     9721 ccagttttta aatactttta caacctcatc atattttgct atttcttgaa tcattgttat
     9781 acttccaata atattgataa aatcttctgt atacgcctta acataatctt ctaggctaga
     9841 tacgatattg ttttgaattt cttttatcaa cgtatctaaa actaatcttg catctgcact
     9901 aattggatat tggaatgctg atgtcttatc tttaacttca tctaattcta gtacttgctt
     9961 taataagcct tccctctcat ctgatgcaag tggcttacaa agatgaatat tttttccatt
    10021 atcttttagc aattcttcaa ataaccaagc tttttcctct atattactgt ctaaattttc
    10081 gcataattgt tcatattttc tttttataac attatatatt tgtttttctg ttcttcctaa
    10141 gaaacttttt gaaatgaaaa aagttatact acccttaaaa ttttctttat gaagacacat
    10201 atttaactgt tggaacaggt ctagtacttt tgtaagtcta gaatctaata tatctgcttc
    10261 tggtatcaat acaattactg cacatataga tgcagaattt ttaaatactt cacctaatga
    10321 aactgctgca ctaatatcat gggctactcc cctattgtcg ctaaaacctg cagtatcaca
    10381 taattttgca tccattcctt ctattggttt tctcatattt acaatgttcg ggtataaagt
    10441 ttgagaaaca gctcctgaac caatctctgc cacagggtct aagcaatcaa tatatgatat
    10501 accagtatct tcatcttcta cttccatcat tacacatcct gcaagatagt taattgcagt
    10561 acttttgcca catcctgtcc caccaagaat tagaattaaa ttttgatttt cagccttatt
    10621 tattgctact tttccttcat ttaataagag tgttatttct tctaaaagtt gattgttatt
    10681 ttctggcatt tattttcccc cttggttata tattttattt aaataaagtt taatccctta
    10741 agtagattgc aaagttttta tactatattt aaatttgttt tttatttttt atttttaata
    10801 aatttgcatt ttcactaaat ttgtattttc actcaataat tattcttctt tgtttattaa
    10861 aataactgtg ttgtataata taatctttat caacacagtt attttattta atgcgcttat
    10921 tttattgtat tttatgtaat acagttgttt tttatattat atgtccatta ttttcctgtg
    10981 gttattacat atagattttt aattatattt gtaccattaa tttaaacatt cctatactac
    11041 tgcttaagtt ttacacctat accagcttga atattttatt tttatttgct taaatagttc
    11101 aactatactg gtttaaatag gttatcttta tttgcttaaa tagttcaact atactggttt
    11161 aaataggtta tctttatttg cttaaatagt tcaactatac tggtttaaat aggttatctt
    11221 tatttgctta aataagttca actatactgg tttaaatagg ttatctttat ttgcttaaat
    11281 tagttcaact atactggttt aaatagtttt cctttagttt aaataattca cctataccta
    11341 cttgaatagt tcacttttat ttgcttaaat tctataccaa tactggctta aacattatac
    11401 taccatgatt gtattgtcta tcttcaacta caataaaaac aggttctaaa tctttagtag
    11461 atgttatttt taattctaaa aaaacattat cagttggtaa tatttttaca tcataattat
    11521 ttaaattatt attcgtgata ctattaaata catcaccttg acgttcaaaa aaaacattat
    11581 caactattac aaaataaaca tcttcttttc tactattttc aacctctata gtattttgtt
    11641 tagttacatt tagtctcatc ttccacctcc tattaatatt acttaattat attaatactt
    11701 gtaattgttg cattatgttc atcacaagga agaggtttaa aaacaactat ctctgctaaa
    11761 taattctgtg gaggatttgt aactgaagta gcattacaaa taaatttaaa atttgttcct
    11821 gctacaactt gagtagaaac aataagtggt tcataaccaa ccccttctaa cattcctaca
    11881 gcttcattaa atacttttaa atcttcttct tcgacagctc tgaattttga ccatccacca
    11941 acaaaaagac tcatataaaa acctccttta tatatatatt tatttatata tatagtagta
    12001 ccaattttct caatattcaa tattttctgt gtaactggtc aatgaaaatg cataactgga
    12061 taaaaatact tataaaataa cttaacttta catataaata gcttaattta catgtaataa
    12121 gattattcta caatatgaaa tacatcttat tttgaatata ttctaaatac aataatggat
    12181 attttcaatt attatactta gaaaatatcc attacttaat ttgttttatt taaaatcaca
    12241 tagagacttt attatttttt ttattgcatg aatagctatt tcaggtatca taaggtgata
    12301 taccccctcc ttgaaaccac aaaaaacata acaaacttta cgaatcttgt cttaaaagac
    12361 cacaggtaca ttccttattt gtggattgga gtagccataa caagcaaatt ttacaatttt
    12421 tatgatgaca aaaatcaata aaatcgctta gtatataaca cgaaatacta cttgttgcta
    12481 ttaagaatag ttagatagga agtatgcatt ataccaataa aggaatcaat aaagaaagtt
    12541 aaaacatccc tacaaattaa aaatctgtta catttttcta tatttaaacg ttatttactt
    12601 acaattggag aaataacagg ttttccttct cggtcaagta aaggcgtaaa tccggctgca
    12661 tggccataat agtggaatac atagttcaca cctgtttctt tatccaccca aatctccgtt
    12721 gcatttattt ttccttgtga atatactttt tcaaatctat cttttcccat tttataatcc
    12781 tccatatttt tgattggtct atgccttctt cacttaagta gaaaaaatac ataacttatt
    12841 tttttaattc taatatgagc acgtaaaaat gtcaaacagc ttatttaatt tcataacttc
    12901 atgttactgt tcgtaacctt gtctgtttac ttacactgca ttacagcacc attttatcct
    12961 ttacattatt gtattgcaca cagtaaaaat gcttacttta ttggattttt caactaaaaa
    13021 aaggattttt tctccacaga acatttacta ttttttataa tatatctaat tttactctca
    13081 tatcaacatt taatgaagat ataacctttt tcaaatattg taaagactcc ttaaaaatat
    13141 tagaaaaaga ttctgcaaat ctctcaacat ctcctttttt taaataccta agtatctcaa
    13201 aacatacatc aaaacgtgtt ttcaaaattt cattatattc ttcatgaaaa aatctctcta
    13261 aatagattcc aaatatcata tattgtttta aatgtaaaaa tgtttcctgt aatacataaa
    13321 tttgtgtata atctactatc ttacaaagta aaacaaaatc tggataaacc ttacactctt
    13381 cacaattatt ttgtaagtac cttaaatgag tttctatttt atctacagct atcaaatcca
    13441 ttttatttcc tagtaaaagt actacatcct caagagtaat cgtaacaaac tccatagcat
    13501 ccaaaagcaa actcaacctt ttagctcctt cttctgtatt tatataatca tcatcatatt
    13561 cagaaaaaag tgttatatat gttcccttgc cattaattgt tttagcaatt cccaattcat
    13621 ttagctcatt ataggcactt ctaactgttg gaagagatac attatatcta ttaattatcg
    13681 aagtaattga tggtagacaa tctcctattt taatattgcc ttcaaaaatt tgacaaatta
    13741 catctgaaac aattaagtca tataaataac atttttccaa cgttacagaa ttaaaactac
    13801 ttgtatctat actttgtgga gcacgatttt ctaccatttt aagtatggat tctctacaag
    13861 ctttatacat atatgttaat agttttttaa attcaattag attcccgtcc aatccatata
    13921 agtaagtttt tcttacctgc tctatgtatt tattacgaaa aaagtctata caacaattat
    13981 ccaagttatc caagttatcg aaattatagg ccaattgtga aataatgaca tcatgctgaa
    14041 taatctcaca aaaatggtga atcaggaaat tatttgtttt acttattagt ttatttatat
    14101 attttagcct ttgttcttta aagactaaat aattagaact atttttttcc atacaatcga
    14161 taatctcgtt taattcttca aagtctttag atgtaaatct tttaatattg tacatagcaa
    14221 tagaaggaaa tattaaacta aaaatcttat atatttctga tatttgtaaa atcttctcat
    14281 ataataagtc agattctatt gattgtttac tcgtactctg gattaaaact ggtctagaac
    14341 cagcttttgt ctctataaag cccttttctg agagcatttt tattacagtt tttatagtat
    14401 tttttgatac catatattct tctgacatgc tttttataga atcaagccta tctccaatag
    14461 taattttacc agtataaatt tgttgaatta tatgctcaaa aataattaaa tattgtggtt
    14521 ttctcttcat atttttcctc ttcgataaat tagatttatt gttttttgta aggtatattt
    14581 acttttattt aatagggcaa aatagttgct tattttttcc tcataatcta taattaatat
    14641 agaccattta tatatatggt ctaagtttta taatactgta atgcagtaaa atagcatatt
    14701 gtagctatac tacagcaaat taacatacca ttgtagtata ttaattggaa taattttaag
    14761 tacttcaaaa ttccagcaaa acatatatgt gtttttgata ttatgtatat tcgttaatag
    14821 aatagctata cttgttttac ataataaatt atatgatact tgctatatgt aattactata
    14881 ttta
//
LOCUS       DAMKMI010000102        14803 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMKMI010000102 DAMKMI010000000
VERSION     DAMKMI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14803
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.106"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            393..1304
                     /locus_tag="PC429_002697"
     CDS             393..1304
                     /locus_tag="PC429_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF3848192.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1479..1976
                     /locus_tag="PC429_002698"
     CDS             1479..1976
                     /locus_tag="PC429_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF3848193.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            2720..3082
                     /locus_tag="PC429_002699"
     CDS             2720..3082
                     /locus_tag="PC429_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF3848194.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            3147..3764
                     /locus_tag="PC429_002700"
     CDS             3147..3764
                     /locus_tag="PC429_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF3848195.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            3761..4246
                     /locus_tag="PC429_002701"
     CDS             3761..4246
                     /locus_tag="PC429_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF3848196.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            4289..4807
                     /locus_tag="PC429_002702"
     CDS             4289..4807
                     /locus_tag="PC429_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF3848197.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            4943..5902
                     /locus_tag="PC429_002703"
     CDS             4943..5902
                     /locus_tag="PC429_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3848198.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            5928..7631
                     /locus_tag="PC429_002704"
     CDS             5928..7631
                     /locus_tag="PC429_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF3848199.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            7892..9994
                     /locus_tag="PC429_002705"
     CDS             7892..9994
                     /locus_tag="PC429_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF3848200.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            10007..10447
                     /locus_tag="PC429_002706"
     CDS             10007..10447
                     /locus_tag="PC429_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF3848201.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            10483..11286
                     /locus_tag="PC429_002707"
     CDS             10483..11286
                     /locus_tag="PC429_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF3848202.1"
                     /translation="MIKLTDEEFIVLVNYVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            11326..11922
                     /locus_tag="PC429_002708"
     CDS             11326..11922
                     /locus_tag="PC429_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF3848203.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            12028..12891
                     /locus_tag="PC429_002709"
     CDS             12028..12891
                     /locus_tag="PC429_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF3848204.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            12958..13947
                     /locus_tag="PC429_002710"
     CDS             12958..13947
                     /locus_tag="PC429_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF3848205.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYIEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            14039..14650
                     /locus_tag="PC429_002711"
     CDS             14039..14650
                     /locus_tag="PC429_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF3848206.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         6058 a         1382 c         2730 g         4633 t
ORIGIN      
        1 aattaaataa gacgataaca agtgagataa taataaatga attgacatag caataagtaa
       61 attaagtaag acgataacaa atgagatgat aataagtaaa ttgatataga aataagtaaa
      121 ttaagtaaga tgataacaag tgaggtaata ataagtgaat tgatatagaa ataagtaaat
      181 taagtaagac gataacaaat gagatgataa taagtaaatt gatatagtaa taaataaatt
      241 aagatgataa tatccaaagt gagaaagata atagaaagta aataaaatca tgtaagaata
      301 atttagataa gattctaaga ttttatttat ttaaaaatta gtatgattaa tttagattaa
      361 ataaatttaa taattttagt aggtggtaat gaatgaaaat aaatagatta acagaaataa
      421 tagtaattct attaaataaa aaattagtaa cagctaaaga gttggcagat agatttgaag
      481 tttctacaag aaccatatat agagatatag aaactctgtc tatgtcggga gtaccagtat
      541 atatgacgaa aggcaaagga ggagggatat ctttaataga agaatattct atagacaagg
      601 ctatattgtc aaaaaaagat aaagagagtt taatagtggc actgaaaaca ttacaggcga
      661 ctaagtatcc agaaataaat tcagtagtaa ataaaattgg ctcaatattt ggagagcaga
      721 atttttcaaa ctggattgaa atagacttta ctgaatgggg aagtaatttt aatgaagatg
      781 ataaatttac aaaaataaaa gaagctattt taaggcgtaa tactataaat tttaattatg
      841 taaatagttt gtcaagccag acaaacagaa ctgtcgaacc actgaagctt atgtataaga
      901 gtaagacttg gtacttgtat ggattttgta aactaaaaga tgattttagg atatttagaa
      961 ttagtaggat aaggaattta tctataaagg atgaagtatt ttcaagaaag ataatagaag
     1021 aagtatgttt gaatgattct aaagtaatta aggaaaatac tataacgttg aaattgaggt
     1081 ttaaagaaaa gatgcttttt agagtatttg atgattttaa taaggattta ataactaaaa
     1141 atgaagatga tacatatgat gtaattacag aatttcctat tggtgaatgg atttatggat
     1201 atattttatc atttggtgac aatgttgaag tacttgaacc taaagatgtt agggataatg
     1261 taataactag actaagggag ttgtctaaaa tttattcatt ataatataat gctagattaa
     1321 agattttatt aaatatataa tatcttgcat ttagtatata atatctcact gagtttaatt
     1381 atattaaaaa tatgacataa aggtgtcgta tattataaat tataattgaa ttagttcaaa
     1441 ataaaatata ttaataaaaa caaagtgagg tataaagtat gaattatgaa atagtagaga
     1501 taaatgagaa aatagttata ggaatatcga aggagactac aaataaagat ggacaagcgg
     1561 taaatgatat aggtgaattg tggaaaaaat ttatgggcaa agggatatat aatgctataa
     1621 agggtaaaaa gaatgacaag acaataggtc tatatacaga ttaccagggt gattttacaa
     1681 gtccgtatag ctttgttgca tgttgtgaag taaatagtaa ttctgataaa gagaagaatt
     1741 tagaagaatt aaacactaat aatactgttg gagaaagtat tattagtaaa gttataccag
     1801 caggtaagta tgcaaagttt gttatagtag gaggtcaaaa agaagtagga gatttttggt
     1861 ttgagttttg gcaaatggat tttgatagga cttatataag tgattttgaa gagtatcaat
     1921 gtaatacttt tgatacaaag aaacaagaag ttcatattta tataggaata aagtagattt
     1981 gttattacta tattgaatga agtattgagt atagtacatt tattatagat agttttatta
     2041 tttataacta taagcagtaa atctgtaaaa gatttactgc ttttttagta attaatatta
     2101 tcagtatcac tttgaatctt cagacatatt cttcatttaa tattgtttat tgataaatga
     2161 tacttgtaaa tataatgatt tgttatttaa tttaaaatat gaaaaaaagt attataaaca
     2221 cattagaata cttctggatt tatgagtata caatttgaaa aatataaaaa ataatgtata
     2281 aagctaaatc taatttaatc gataatacat atagagaaat ataattaagt aaataaacaa
     2341 ctatctatta gatattgaga aagtcaaatc ctatatgaat ctacagaaat gattttaatg
     2401 aatctgaaaa atttttttat acaaacataa aaaaggtata aagttgtttt attataattt
     2461 aattactttt gataactttt tatgttttat acaattatat ttttaataac tcgtcaaaac
     2521 gccaaacata tattacaata gatatataag tttttttgtt gacaaattat gttaagagaa
     2581 ttaagtataa tatggcataa ataattactt ttatttatca atgaaatgta tttattgtgt
     2641 agcgaaaaat agtcagatag tatagacaat aactgaagat aaaagcgttt attgttgtac
     2701 atatggagga gttagtttta tggaaaataa aattttgata gtagatgatg ctatttttat
     2761 gagaatgata attaaagaag ctctgagtaa aagtggctat aataatttaa tagaagcaaa
     2821 tgatggagaa gaagcgtgta atatatttaa atatgaaaag cctgatttga tgttgcttga
     2881 tattacaatg ccaaaaaaag atggtttaga ggtgctaaga gaaataaaaa agttggattc
     2941 aagtgctaag gttgtaatgt gctctgcaat aggacaagag aatatgattg tagaagccat
     3001 aaaacttgga gcgttagatt ttatagtgaa gcccttcaaa acagaaacat tagttaaagt
     3061 tgtagataat gctttaaaat aaaagtgtaa atatacacta atacaatgaa aagcaaataa
     3121 atgcaataat aaagggaggg gtttatatta taaattattt agaacttgat gatgttcata
     3181 tagatgctct tagagaaatt ggtaatatag gctctgggaa tgcaattact gcacttgcat
     3241 ccatgataaa tagcaatgta gaagtattaa tacctatggt caagatactt gaatataatg
     3301 aagcgacaaa tttgcttggt ggtccagaaa acaaagtagt atgtatttta ctcgatatga
     3361 aaggtgatat caatggaatg tttatgtttt tgttggatga atcaataact caacttatgc
     3421 taagttctct ttttaataaa gaagaagctt ttttagatga gattgaggca attgaaattt
     3481 cagcaattaa ggaaatagga aatattatgg caagttcata tgtaaatgct attgcatcta
     3541 tgttaaacat gactataagt gtttcaattc cagatatatg tattgatatg gttggagctg
     3601 ttctaaatgt acctatgata agattttcag atgttggtga caaagtctta tttatagaaa
     3661 ataaatttaa aatgagtgat aattatttta ctagtcacat tttgatgatt ccagaaatga
     3721 gctcacttag agaaatactc gtgaggttgg gattagaagt atgaatagag agatagtagt
     3781 gaacattgca gatatgaaga tagcctatag accaaatgtt ttagttactt atgcacttgg
     3841 ttcttgtgta ggagtatgtt tgatagataa agtagctgga attggtggaa tgttacacgt
     3901 tatgttgcca tatagtaaag atgctattaa cattgaaaat aagtgtaaat ttgctgacac
     3961 aggaataaat gagctcatta aatctatgga gaatataggt gcaaatagaa tatatataaa
     4021 agcaaaaatt gctggaggtg ctcagatgtt tttaggaagt agcaattcag taattgcaag
     4081 tataggacat aggaatgttg aggcagtaaa aaaaactctt ttaggattaa atattccaat
     4141 attagctgag gacacaggta tgaattatgg tagaacaata cgtttttaca ctgagacggg
     4201 tgaattactt gtagattcaa taaataaagg taagaagaaa ttataatata tatttcatag
     4261 aaagtgtatt ttaggaggga ggtattaaat gagatttcca gatgacccat atttagatta
     4321 tggactaatt gatgaaggaa ttaagtatct aaagtttaga gtagatgagc aagactttgg
     4381 ggttgaactt gaaaaggtaa tagaaattac tggaaatcaa gaaattatat ttattccaga
     4441 actacctaca tattctaaag gcataataaa tctaagagga aaaatcattc cgataataga
     4501 catgcgtttg agacttaaaa aagatgaatt aatgactaca aattgcatgt atatagtagt
     4561 aactgaaata aaggacttgg tagttggatt tgtggttgat agagttgatg atatagtaag
     4621 cttagaaaaa agtaaaatat cacctccacc aagagttact atagaatatt cggattattt
     4681 tgtaagtgga gttggagaat tagaaggtaa aataattact ttactagatt tagagaaact
     4741 tttgacagat aaagatgaag ttttaataga tgaattaatc aatgggtttg attgcactat
     4801 catgtaacat agggatgtag ttggagtgtt attataaaaa gattgtgatg agaacattta
     4861 agaatatagt ggtataatta ttaagtataa aaattttaaa tcattaaaat ttgaatatat
     4921 tatgtttaag agaggtttgt atatgaaaaa taagcaacag ggaacaattt tagtcgttga
     4981 tgatagtgtt ctgatgtgtg acttaataga taaaacactt acaaaagata attttaaggt
     5041 taaaaaagca tttaatgcaa tagaggcaaa agaatttatt gaagaattta taccagacat
     5101 tattttgctt gatataattt taccagataa ctctggtttt gagttttgta aggaagtaaa
     5161 atctaatgct cgtacagcag atatacctat aatttttata acttcaaaaa atacagatac
     5221 agacatagta aaaggatttg gtgtaggagc aattgactat atggtgaaac cttttagtat
     5281 gacagaatta aaagctcgtg ttatagctca tcttgaagta aagaaatctc aagataaatt
     5341 aaaaaagata aataatgaac tggaatcttc tcttgaaaaa ttgaatagac tcgttgtaag
     5401 agattatctt acaggtcttt acaataggcg acatataatt aatcagctta tggagcaaag
     5461 aagaattaat aaatatagtg aaacatcaat atcccttatc cttggtgata ttgatgactt
     5521 taaggttatc aatgatactt atgggcatga agcaggggat ttcgtgttaa cagtaatatc
     5581 aagtataata aaaaagaatt gtagacaaat tgatactgta tcaagatggg gtggagaaga
     5641 gtttttgata atgctcacaa atactccaat agaaggtgct aagatacttt cagaaagaat
     5701 ccgtaaagag ataaaggagt ttgatttcaa ctataaagaa cataaaataa agtgcactat
     5761 tacattagga gtagtagaag taaactcaaa tatatcatta gaagaaaaca tatccttagc
     5821 agataaagca atgtatgagg gtaaaaactt aggaaaagat tgtagcgtag tgagtacaat
     5881 gaaaggatta aaaaagatat aaacttactt tatgaaggag tacagatatg aaaagagaag
     5941 taaagctttc taaattagca attgtattgc tgattattat agtattggct gttctagcag
     6001 ggattttttc ttctatttca tcttataaag catttgaaaa agtagaaaaa agtattaatg
     6061 atatgaataa tttgaggact caaagtcagt atataaaaga tacaactaga gagataagtg
     6121 atgtattaag aagatatgta tttacaggag aaaaaaagaa taaagatatt tatgaaaaag
     6181 aattatacgg aaaaacaaga gaatcagctt taaacaaatt agaaaaaatt ggtcttacaa
     6241 agagcgagtc aaataaaatc ttagaagcaa aagaaatatc agattcttta gtaccaacag
     6301 aacaaagtgc aataaaggca gtcgaaagta atgataagag taaagctcaa agtataattt
     6361 ttggtagtga ctatataaac tcccaagaga aaatggattc tttaataaat gaatttcaag
     6421 aggatatagt tacccgttca aattccaaaa ttgtagaggt aaaaaaagaa ttacaaagtc
     6481 taatattaag aacaatattt atactttccc ttttaataat attgacaata atagaatata
     6541 cagtaataaa attaaaaata gtgattccta ttaataaagt agaaaaacac ttttcaagaa
     6601 ttgcatctgg ggacttaaga acaagtatag acgttgaaga aagtaaatct gaaatagggc
     6661 tattagtgag ttctgtcaaa aagatgcaaa gtatgctttt aaattacata gaccttatag
     6721 acagtacatt aactagtatt gcaaaaggag atttaagagt agagatagac caagaattta
     6781 taggtgattt taaatcaatt aaaatatctt tagaaagtat aataaactct tttaatggtg
     6841 atttttctga aataaattta tcagcagagc aagttgcaag tgcttcagaa caagttgcag
     6901 caggtgcaca agctctttca cagggtgcta ctgaacaagc tagttcagta gaagaattag
     6961 cttccacgat taatgagata tcagatggaa ttaagaagtg tgcagatagt gctagtcttg
     7021 catgtgaggt atctgaccaa tctgcatcac aagttggttt tggaagtaga tgtatggatg
     7081 atatgatgac atcaatgggt gaaataagtg aaaaatcgag tgaaatatca aaaataatca
     7141 aaacaataga tgagatagca ttccaaacta atattcttgc attaaatgca gctgttgaag
     7201 cagcaagagc aggtcaatat ggtagaggat ttgcagttgt agcagatgaa gttaggtctt
     7261 tggctggtaa aagtgcaaaa gcagctcaaa atacagctat tctcatagaa gaagctatta
     7321 aaacagtaga aaatggtgtc agaattgcta aagagactgc aaatgcattg gacttggtag
     7381 taaatggtgt agaaaaaact actaattata ttaatggtat ttcttcggct gtacatagtc
     7441 aagaagtagc aattacacag attttatctg gaatagagca aatttccata gtagtccaaa
     7501 tgaactcagc aacagcagag gaaagtgcag cagcctcaga agagatgtct gctcaagctc
     7561 aaattttaaa agaattagta gaaaaatttc atctaaagga aagtgaagta agagaatcat
     7621 catgggttta aattatgagg tgaatattga agtaagagaa ttatcatgta tttaaattat
     7681 gagatgtata tatattgaaa taagagaatt atcatgtatt taaattatga gatgtatata
     7741 tattgaagta agagaataat tgtgtattta aattatgagg tacatatagt gagataagag
     7801 aatcatcaag gatttaaatt atgagatgca tatataatag aaaattattt gtaggtcgta
     7861 ggtgataaat gtgaattata ttgaaccgag tatgagacaa atgttagata tgtttatatt
     7921 tgagacaagt ttattactcg aacaattaga tgaaattatg attcaaatag aaaaagagaa
     7981 agtatttact cataacaaca ttaatgaaac ttttagaata atgcatacga taaaaggttc
     8041 agcatctatg atggggttag acaatatatc tgctttagca catagtgtag aagacttgtt
     8101 ttatgctata cgtgaaggta aaatagaagt tttagacaat gacctattat ttgatttagt
     8161 ctttcaatca ttagatttta taagaagaga tatagatgag ttacaatcaa gaggttgtat
     8221 atcttctgat tactctaatt ttattgaagg aattgaagag tatgtgagta ctttggaatt
     8281 atcaagttct atgaaatcaa aggataatgc aaatttagaa agtaaagacg aaaaagaagt
     8341 aatcgattca aattttgaat acaaagaaga ctctgatgta gtaaaaataa tctttgaaca
     8401 aggttgcatg atggaaaatg tgagagcttt catgcttgta tttgaactta gagattactg
     8461 tgaatttata gatttttatc caaaagatgt agaaacaaat gcagagtcta cagagtatat
     8521 taaaagttat gggttttata tacaatttgt agctaaagag gataaggcaa tagtttataa
     8581 aactatagaa gataatctct atgtaaaaga atacataaat attagtaaaa aagaaataga
     8641 aatgaaaaat acatcaaaca atgatgcagt agaaaataaa ttaaaagata ataataaagt
     8701 aagtaaaagt tatcaaaata actatacaga agatgagata caagaagatg ttttaaatga
     8761 aaaaactgat atggaaaaag atagtctaag tgaagaaaat gaacatttac caataagtaa
     8821 gcagagtatt atgagtgtaa atttagaaaa attggactta cttcaaaata tagttggaga
     8881 aattgtaatt atggaatcaa tggttgtaaa tagcgtaaat tctacaggtg ttgatataga
     8941 taatttcaat aaggcaacaa gacaattaca caagcttaca gatgaattgc aagatatagt
     9001 aatgtctatg cgtatgattc aaattgctgg tgtatttcaa aaaatgcata gaattgtacg
     9061 agatatgaat aaaaaattag ataaaaatgt agagttaata actataggag cagagacaga
     9121 ggtagataag agtgtaattg ataatttagc agaacctttt atgcacctta taagaaatgc
     9181 tatggaccat gcaatagagc ctattgagga gcgtatagcg aaaggtaaag aagaagtggg
     9241 aaagatagtt ttatctgctg aaaatctagg cggagaagct gtgataagta tttcagatga
     9301 tggtagaggc attatgagag ataagatttt ggcaaaagct aaggaacaag gtcttcttaa
     9361 acatcaaagt aatgagtata ctgatgacga ggtcaattct ttgattatga ctccaggctt
     9421 ttctacaaaa gaatctataa ctgaattttc tggaagagga gtaggcatgg acattgttcg
     9481 taaaagtatt gaaaaagttg gaggttcaat tgaagtaaaa agtaaacaag atgaagggac
     9541 aacctttact ataaaaatac cattaactct ttctatagta gagggtatga agtttaaagt
     9601 tggagattca ctatttactt taccaatatc atccataaga agaacattta aacttacaga
     9661 cttaaatcaa ataattacag aaggaaataa gacagagtta attacaatat atggagcatg
     9721 tattcccatt ataaggttac ataaaatata taatattcaa acaaaagtaa ccaatctgat
     9781 gaatggtaat atgataatta ttgaaaatga gcataaagca gtatgcattt ttgttgatga
     9841 aataataggt aatcagcaag ttgttgtgaa accatttcca gtttatttta accgatttaa
     9901 tataaaaaat aagggtttat cgggatgtac aatacttggg gatggaagta taagtttaat
     9961 tatagatgca gatagcatta tagaaatata ttaggggatt taaagtatgg ctaaagatga
    10021 aattattaaa caagtattaa cattttatgt caatgatgtt atttatggaa tagaattaga
    10081 aaatgtgatt gaaaccataa gatttcaatc aataacgtat gtaccatgtc ttccaacata
    10141 tataagtgga gttattaatt tgagaggaag aataatacca gttattaata tgcatataaa
    10201 gtacaatctt cctaaagctg attataatga gagaacttgt ataataatta caaaggttga
    10261 tgagtatcaa gttggaatta ttgtagataa agttgttgat gttattcata tagaaaatct
    10321 aaatttacta gagacgacta attctaacaa cactaatatt aataaagata taaaagagat
    10381 tgcgaaagtt gaagataatt ctatattaat tttagatatt cgaaagtttt tgattaatag
    10441 catatagtta gttctgataa attagcagga ggaagttttt gcatgataaa actcacagat
    10501 gaagaattta tagttcttgt aaactatgta aaaaaagaat atggtattga tttaagtaaa
    10561 aaaagagcgt taattgaagg tcgcttatac aatacaatga tagagaaaaa acttagttct
    10621 tttagtcaat atatgaatct attatttaaa gataagacag gaaatgaagc tataaaccta
    10681 ataaatagat tgagtactaa ccacacattt tttatgagag aacctcaaca ttttgagttt
    10741 atacagaata gtatacttcc tttttgggaa gaaaataata aaataagaaa cataaatata
    10801 tggagtgcag gatgttctag tggagaagaa gcgtattcaa tagctatgac tttggatgat
    10861 tattttggat ataataaaga gttgtggaac attaaaatca tagccacaga tatttcaact
    10921 aactcacttg aaaaagctaa aagaggtata tacatagaat caaatgttaa caatgttcct
    10981 gagttatgga agaagaagta ttttatagac aacaaggatg gtacatttaa aatttgtgat
    11041 aagattagaa aaagtgtaat ttttaagccg tttaatttaa tgaatgattt ctcatatcaa
    11101 cattttgatt tgattttttg tagaaatgtt atgatttatt ttgatttaaa aacaagagaa
    11161 gaacttataa ataaatttta taatgctacc aaaaaagata gttttttgtt tgtaggacat
    11221 gcagaagtaa tcaacagaag tagtactaaa tacaaatatg taaaacctgc tatatataaa
    11281 cgataaaaag gtatttttga tatatgattt tttggaggcg gtaatgtgga acgttttaat
    11341 aatatggtta ttgctatagg cgcatctgta ggtggtacag aagcaatact tgaaattata
    11401 aaagacctac caaaatcaac accagggatt gttgtagttc aacatatgcc agctatattt
    11461 acttatatgt atgcacaacg gcttgacaag cagtgtatta tgaatgtaag agaagctaag
    11521 aataatgata gagtcgaaca aggaaatgtg ttaattgcac ctgggggtta ccagatgaaa
    11581 ttgtgtacag ataaacaagg atattatgta acttgtgaaa agggagaaag aataagtggt
    11641 cactgtccat cagtagacgt tttgtttgat tcagtagcag aagtagcagg taaaaattca
    11701 ataggaataa tacttacagg tatgggttat gatggagcaa atggactttt aaaaatgaaa
    11761 gaaaatggtt catttacaat tggacaggat gaaaattctt gtatagtata tggaatgcca
    11821 atggtagctt ttgatagagg ttctgttatg ttacaattac ctcttggtga aatttcaaac
    11881 tgtttgataa gacatattaa tattttaaaa caaaaagatt aattttttat aaataaaaat
    11941 taatctttta ttaaattata tggataaaaa accctagaaa ctccaaatat aataagagag
    12001 atttatattt aggaggaaaa tgataatatg aaatttttta aagtcgatac aaaagctaaa
    12061 gttttaagat atgtatttgt tttaacattg actataatgg ctgttttggc aattaaacat
    12121 gactataaat ataataattt aaggaaagat gcttcaatta atttaaatag taataaggaa
    12181 gcagtgactg ttatgagtaa ccagtcatta ccaaagggta atacaaatag taaacaaact
    12241 ttatcagtaa gtacaatagg aagtaaaaca atagtgaaaa atgatgagta tttagaaagt
    12301 actataaaca tgcctgtaat aacaaattca aataaaattg ttgaaaggtc tacaaatgat
    12361 aagataaaaa atgacatatt tgaattttat aataaatcat ataaagaagc aaagcagtat
    12421 cttaaggata atcctgacga gaaaaataag tttgttgcaa atgtagactt tgaacttaaa
    12481 aagaatactg attctgctct tagtataaaa gtgagatatt atacttatag tggtggagca
    12541 catggatttt atcaagatat agcatataat gtggatatga gaactggaaa atttttagaa
    12601 ttaatggact tgtttaagga taatactaaa tataaagaag ttatagatga agaaattaaa
    12661 agacaaatag ctgaacttga aaaaaaggat gaagaaaata tcggaatata taattttaaa
    12721 ggtataaaag agaatcaaaa cttttatttg caagatgaga atctagttgt atattttgat
    12781 ttatatgata taactccata tgcagcagga ataccagaat tttcaataaa taaagcgttg
    12841 ataagttcta tgctaaaacc agagtatgta gatttatttg atttgaaata gaagattaaa
    12901 ttaaatgtta tgctataata tttagcatag agacttatta aaattggggg tataaaaatg
    12961 ctacttttaa aaaaagacga cataaaaaaa gtatttacaa tgagagatgc aattgaagct
    13021 gataaggaag cttttagaat ttattgtgaa ggaaagagtg taaatccttt gagaaccaat
    13081 atatctgttc caagtcaaga tgctagtatg ttatttatgc caggatacat agaagaatta
    13141 ggctgtgggg gaataaaaat tgtatctgta tttcctaaaa atgctcaaaa aggcaaacca
    13201 gtaattccag caacagtact attattagat ggagaaactg gagaagttag tgcagtattg
    13261 gatggcactt atgtaactca gattcgtact ggagcagcct ctggagcagc tatagacgta
    13321 ttggctaaaa aagattctaa gattggagct ttgataggtc taggaggaca aggtgagcca
    13381 caacttgaag ccatgataga agctagaaat ttggatgagg taagagtttt tagtagaaac
    13441 aaggaaagta gagaaaaatt tgcagaagaa atgaacttaa aattaagtaa atataatact
    13501 aaaatagttg cagttgaatc atctgatgaa gccataaata acgcagatgt gatagtttta
    13561 gctactccat caaaacaacc agtacttaat ggaaacttag tgaaaaaagg agctttaata
    13621 agtgcagtag gttcatatat gccagatatg caagagttgt gcccaaactg tttaactaga
    13681 gcgtctaaga tattttttga atcaacagat gcagttttat ctgagtcagg agatatatta
    13741 attccattaa aagaaggtaa gataagtaaa gatgatttta gtggtgattt agggaatgtt
    13801 ataaacggaa ctattcctgg aagagaagat gatgatgaga taattgtatt taaaactgtt
    13861 gggataggtg tacaagatgt cgtaacagca aaaagaatat acgataaagc aaaagaaaac
    13921 ggtattggaa tagactggaa gtggtaaaat attcaaaata attctatagt tagacaaatt
    13981 tatgttataa tgttgataaa taaatttttt attattttaa ttgagggagg atgtttgtat
    14041 gaaaatggta aaaaagttaa tattatctac gattatggct ctagttttag ttctaactat
    14101 agggtgttct aatgcagaag aaaaagtaaa gtcgaatttt gaagctaatt tagctgcatt
    14161 agaaatgtat aagtatgatg aaaaaggaat ttctgatgca ttaacagaag ctgaatataa
    14221 acagattcaa acttataaca gcaaggacaa ggtttcaatg attgctcaat tgacacctct
    14281 ttatggtaaa gaaaatgctg aaaagatata cgaaacaatt gttaaattaa ttcaaggtgt
    14341 tgaaaagaca gtaacagtgg aatcttctag taaggataag ataactttga aagttgtaag
    14401 taaagcttta gatactagta aagtaatgag tgaagcgaac actttagtaa aatcttatgt
    14461 aactgatgaa aagaattacg ataaaaaaga aaaagaagtt caagatgata tgacaaaaat
    14521 gattatagac ctttacaata agaaaccaac taaggaagtt acaactatga ttacatatac
    14581 taaaaaagat ggtaaatggc aaccacctag taatatacaa aagttatgtg aaggaatata
    14641 tacaatttaa tttattttaa agacacaaaa ctatctttta agcatataat aaaatgtttg
    14701 gaagatagtt tattttattt tcaaagtata aattatattt tataaatgat ttattttata
    14761 aataatttat cttgataaat agtaaataat ttactttact aat
//
LOCUS       DAMKMI010000103        14802 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMKMI010000103 DAMKMI010000000
VERSION     DAMKMI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.107"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            106..1173
                     /locus_tag="PC429_002712"
     CDS             106..1173
                     /locus_tag="PC429_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF3848207.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            1192..2352
                     /locus_tag="PC429_002713"
     CDS             1192..2352
                     /locus_tag="PC429_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF3848208.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            2365..4461
                     /locus_tag="PC429_002714"
     CDS             2365..4461
                     /locus_tag="PC429_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF3848209.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(4620..4964)
                     /locus_tag="PC429_002715"
     CDS             complement(4620..4964)
                     /locus_tag="PC429_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF3848210.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            5256..6146
                     /locus_tag="PC429_002716"
     CDS             5256..6146
                     /locus_tag="PC429_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF3848211.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            6364..7650
                     /locus_tag="PC429_002717"
     CDS             6364..7650
                     /locus_tag="PC429_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF3848212.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(7739..8182)
                     /locus_tag="PC429_002718"
     CDS             complement(7739..8182)
                     /locus_tag="PC429_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF3848213.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(8206..9000)
                     /locus_tag="PC429_002719"
     CDS             complement(8206..9000)
                     /locus_tag="PC429_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF3848214.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(9566..10042)
                     /locus_tag="PC429_002720"
     CDS             complement(9566..10042)
                     /locus_tag="PC429_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF3848215.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(10143..11312)
                     /locus_tag="PC429_002721"
     CDS             complement(10143..11312)
                     /locus_tag="PC429_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3848216.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            11475..12338
                     /locus_tag="PC429_002722"
     CDS             11475..12338
                     /locus_tag="PC429_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF3848217.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            12409..13002
                     /locus_tag="PC429_002723"
     CDS             12409..13002
                     /locus_tag="PC429_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF3848218.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(13253..13888)
                     /locus_tag="PC429_002724"
     CDS             complement(13253..13888)
                     /locus_tag="PC429_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF3848219.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(13968..14699)
                     /locus_tag="PC429_002725"
     CDS             complement(13968..14699)
                     /locus_tag="PC429_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF3848220.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEGDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
BASE COUNT         5614 a         1749 c         2382 g         5057 t
ORIGIN      
        1 aaattttcag taaattatct tgacatatat attgaagtaa taatataatt taaagtataa
       61 aattttaaaa attgaattat aacttcaaaa aataaggagg gtaatatgaa gataacagat
      121 atcaaatttg aaaagcttag aattaaatta aaaaaacctg tagtagtttc atttggagta
      181 attgagtatg gagaaagtat aattttaaaa attgaaactg atgaagggta ttgtggattt
      241 ggcgaagcag cacctcttgc agcagttaca ggtgaagttt tggataatgt tctttcagta
      301 ttgcttatgt ttaaaaaaga gttaataggc aaagacccac tagatattga aaccatacat
      361 accatcatgg atggaattat tataggtaat acttctgcaa aagcagctat tgacatagct
      421 ttatatgata ttaaaggaaa aattatgaat gcacctcttt ataaagttct tggaggattt
      481 gatagtaaag tccaaacgga tattactata agtatagata agccagaaaa aatggcagta
      541 gaagctttag aaagagtaaa agaaggtttt aggatattga agcttaaagc aggtataaat
      601 cctgaagacg atattgaagc tgtaaagtta attagagaag ccgttggaga gagtataaga
      661 attagaattg atgcaaatca aggatggaac gtaaatagca gtataaatac tatcaaaaag
      721 ttagaagaat ttaatgttga tgcaattgaa caagcacttc cacattggga tttagatggt
      781 actgcttaca taagaaataa aagtaacaca aaaattatga tagatgaatc attacattca
      841 ccaatagatg caattaaggc tataaaaaag aatgcagttg atacatttaa tattaagctg
      901 atgaaatcaa gtggaatata tcctgctata aaaatcaaca acatagctga ggcaagtgga
      961 gtaaattgta tgttaggatg tatgctggaa acgagaattg gtataacagc agcagcaaat
     1021 ttaatagcat ctaaaaagaa cataacagaa gctgaccttg atagttttat gttttgtgaa
     1081 gaattagaag gtatatctgg aggatttgta atggatggag atattatgaa tcttgtcaat
     1141 aaaccaggtt taggaataga agtaaactta tgataaattt ggaggttaaa tatgaataca
     1201 cttttttcaa atccaggtag tatacttgct gtaatgacat caatgattgc attgggtttt
     1261 tacttacaaa gatacaaagc cattaaaagc ttaggaccag cacttacaat aatcataatg
     1321 ggaataatac tttcaaactt aaaagtagtt ccagtaagta ctgagttata tggaactatt
     1381 tcaacatatg ctattccaat ttctatgact ataatgttga tgagtgttga cttaaaagaa
     1441 atgacaaaat tatcaagaga acctttaatt gcaatttttg tagctgtatt aactgtaagt
     1501 atcatggcat ttttatttgg attagtattt gcagaaaaaa tatcagaagg ttggaaggtt
     1561 gctggaatgt ttgtaggtac atatactggt ggaagtgcta atcttacagc tattggaaca
     1621 ggtcttaatg taagtagaca aactcttgca gcagcaaatg cagcagacta tgtaattgga
     1681 gtaccaacat tgatatttat gtttgctcta ccagcaatat taaaaaattc aaagaagttt
     1741 aaaaaacttt ggccatatca tgtagaagaa tcagaattag aagattgtca aaatgaagaa
     1801 tttatggagt caaaagaatg gagtattaaa gatatagctt ggatgttggc tataggtttt
     1861 gttgttacag aagtttcaac aatacttgct ggatatttca attcaagttt tagtagtgca
     1921 gcaagaatac ttttagttac aacaatttca attataattg ctcaattaaa gccagtaaag
     1981 aaactaaagg gaaatttaga tttaggatta tttgttgcat tattcttttt atgtacaata
     2041 ggtttttcag tagatataaa agagttttta ggttcaactt ttactataac actttactgt
     2101 tttagtataa tttttgcttc atttgttttc catttaggca taactagact tttaaagata
     2161 aagtatcaat atgtaatact ttctatagta ggagcaattg ctgatggtcc tacttcagct
     2221 ttagtagctg cttctgcaaa gtggaattcg cttgtaagtg tagcagtcgt aatgggagta
     2281 attggtggag tattaggaaa ttatgcaggt atatcagtgg catatgcaat aaagatgtct
     2341 ctaggattat aggggggagt ttaattgata aaatttaaat tatacatatc tggactatac
     2401 agtggaaatg taatctttga tggagattta ttaattgaaa agttgaatcc ttttacaaat
     2461 aaaatagaaa gtttaaaacc aataagcaaa gaagaaaata catattattt aaatcttaca
     2521 aaaatagatt taaagtcttt atttaataat tttgatgtat atactaaaag cttagtaaac
     2581 acagataaag acacagttat aaataactta ggagaaacgc attctaaact taatgaatat
     2641 atatgggttc aaagaaacaa aaagttccca ctagatataa ttattgtaga taataaaata
     2701 gtaggtttta tctgtttatc tagagaaact tgtactatat taattatgga tggatatgaa
     2761 gagtatactg tattaaaaga atgggaaaaa actcataaaa atgaagagat atattctata
     2821 agatttggtg gaaattatat gattgatatg aaagatggta taaaactttc tacagatgta
     2881 tatctccctg attttgtaga ttctgccaaa aaagcaccga caattcttat gagaactcca
     2941 tatggaaaag aaaatgataa agaaatatat tataaatatg tacaaagagg atatgctgtt
     3001 gtaatacaag atgttagggg aagaaatgaa tcagaaggta agtgggaacc tatgattcat
     3061 gagagggaag atggagatag taccataaat tggatagtat cacaggaatg gtcaagtgga
     3121 atagttggaa tgcttggagc atcatatctt ggttatgtcc aatgggcagc tgcatcttca
     3181 ggcaataagc atcttaaagc tttagttagt atagtaacat caggaagccc ttttattgat
     3241 ataccaagaa aaggaggagc ttttgtatct ggtatgcttg catgggcatt tatggtatca
     3301 agaaataaag ttgatagaag caaaatggta agagatgatt gggatgatgt tttaaacata
     3361 cgaccaatag aaaatatacc tgttgaagct cttggatata gaattgaatt tctggaagaa
     3421 tggttaaaga gagtagaaaa agatgagtat tgggatttga tggactggca tttacaaaaa
     3481 gataaaataa atgtacctgc attggttgta tctgggtggt atgatgataa tagtatggga
     3541 actacagagg ccttagatgt tataaaggac tatgaaaaag gaaagagaaa ggctattcta
     3601 ggtccatgga tgcataattc aaatacttta agagatataa atggtatttc tttaggaaat
     3661 agttcattaa gatatgattt ggattataat tatctattat ggtttgataa atacttaaaa
     3721 ggtatagaaa ataacataga tacgactgct ccagtagagt attactctgt aggttctaat
     3781 aagtggaaga cagaagaaaa ttggccaata ataaataaga tagataaaag tatgtatcta
     3841 ataagtgatg gaaatgctaa cacatcttta ggaaatggta gattagtatt tgataatgat
     3901 ttagaagaaa agtatgatag ttatatatat aatccaaaag acccatcagt acaactaatt
     3961 gatatgtcag agaatgaagt tggagttcca aataactaca aagatttaga aaaaagaagt
     4021 gatatgttgt gttatacatc agatgctttt agtgaagaat ttactgtaac aggtgatata
     4081 aagttagagt tttttgcatc aagttctgcc aaagatactg actgggttat aaaaattatg
     4141 gacgtggatt tagatggaaa ttcaattaaa ttagctgatg gtatacttag tgctagattt
     4201 agaaatagtt tttataagtc agagtttatg gaagaaggag agatttataa gtttacgatt
     4261 ataacttcaa aaatatcaaa cacgtttaaa gttggtcata aaattagact agatataacg
     4321 tctagtgcta aaaactttat tttccaaaat agcaacacga cagagggata taatagtatt
     4381 gaatatatag aagcaaagaa tacgatttac catggaggta aatatccatc taaattgata
     4441 ttgccaatag agaataagta aatttatata gattaaatag tgaggaggtg tctcaaaatg
     4501 aattttttag ttcgtgagac actttctttt atacgaaatc aaatatattt tcatcatttc
     4561 aacaatgaaa aagaggctta tctctctgtt ttgagatagc ctcctataag ctaaattgat
     4621 tattttgtag ctgcaacaac gctcatttca actaacaaat gctctctagc aagtctagct
     4681 tcaacacaag ctctagcagg ttcaaatcct ttttcaaccc aagcatccca aacagagttc
     4741 atagcttcaa agtctttcat atctcttaaa taaatagtaa cagatagcaa atgttgctta
     4801 tctgaaccat atttgtttaa taaatcttct attttagcta aaacttcagt tgtttgttca
     4861 attactcctc cttcagcatg agtttgtcca cataaataaa ctgtattgtt atgaactaca
     4921 gctctactca ttcttccagt tccttcatat ctctttattt ccatatttta aatacctcca
     4981 tagttaaaaa atctattaaa aatctaaata ataaattaaa aaaagtctat actatataaa
     5041 attatattat atattctgat aataatccat aaaaacatat aactttttag ctataattat
     5101 tattgttatt aattgaaaag atttattagt tacttgacat agtttacact tttaattata
     5161 gtataaattg gaatatttag ataattatta tttaatgtat aagctaagtt tttaattaat
     5221 atggctttat acatttatat agaaaagagg tacgtatgag tagcacaatc aaatcaaatc
     5281 gaataaaagg tataatgttt ataatagcat cagcgcttgg ctttgcaatg atgtctgcat
     5341 tcgtaaagct agctggagat ttaccaagtt ttcaaaaagt gttctttaga aatctggtat
     5401 cagctataat agctttttgg cttatcataa aacataaagg aagtctaact ggtaaaaaag
     5461 aaaatttaaa agtactttta tataggtcaa tatttggaac tctaggagtt atatttaact
     5521 actatgcaat tgacaagttg atgctttcag atgctaatat gctaaataaa atgagtccct
     5581 tccttgtagt aatattttgt gcaattgctc taaaagaaaa aataaatgta aagcagatag
     5641 gagctattat aattgcattt ataggagctt tattcataat aaaaccaaca tttagtgtag
     5701 aagtaattcc atatcttgga ggaattgcag gagctgtatt tgcagcaatg gcatatactt
     5761 gtttaagagt tcttggagat agagaagatt actatacaat tgtatttttc ttttcagttt
     5821 tttcattagt tactgtaggg ccaattgctt ttgcagtata tgaaccaatg acccttatgc
     5881 aacttatata tctattacta ggtggagtat ttgcaagtct aggtcaattt ggaataactc
     5941 tagcatataa atatgcacct gctaaggaaa tctctatatt tgactatagt aatataatct
     6001 tttcagcaat tttaagtata ttcttattca atgtctatcc agatatgtta agtgttgttg
     6061 gatatttaat agtattttcg gcagcatttt atatgttttt atacaataag aagttagata
     6121 aattagacaa agaaaagaag aagtaaacta gataaaagtg tttttataat ttgtatttaa
     6181 tatttaagtc tttgtattaa aattaaagaa aatttaatta aaaatgtttg gtatattaac
     6241 ttataacagc agttaaaatt gaaaaattat aactgctgtt tcttttatta aaaatatcca
     6301 aatgtctgta aaaatagact ataatagagt agtataaata atataaaggg gataaattaa
     6361 agtatggaaa aagtgaaagt tgctgtgatt ggagcaggta atagaggaac gtatgcatat
     6421 gctccatata tttatgaaaa ttctgatgta tgtgaaatag ttgctgtagc agaacctaaa
     6481 aaaggaagac gagaactatt tacacaaaaa tataacctag atagtaaaaa tgtatttgaa
     6541 actttagaag acttttttaa acatgacaag atggcagatg cagtaataat agctacaaat
     6601 gatgatagac attatgatgt agcaaaacta gctcttgaaa aaggttatca tgtactatta
     6661 gaaaagccaa tgtctaatag tttagatggt ctggtacata tagatgactt gtgtgataag
     6721 tataaagata agatttttat gatttgccat gtactgagat attctccatt ttataataaa
     6781 ttaaaagaaa ttgtagaaag caaaaaatta ggagagctag ttagtataca atataatgaa
     6841 aatataggat attggcattt tgcacatagc tttactagag ggaattggag aaatagcaat
     6901 gaaaccagcc ctttaatttt agcaaagagt tgtcatgaca tggatatttt gttatattta
     6961 gtaggaagta gatgcaaaaa aatatctgca tttggaagct taaagcatct taataaccaa
     7021 aatgctagtg gagagatggc acaaaattgt cttcagtgtt tagttgaaaa aaaatgtcca
     7081 tattcagcaa agagaattta tttagaaaaa gatagaagta taaatagagc tgttcacata
     7141 aatccaactg aagagaatct attgaatata cttaagacaa gtccatatgg aagatgtgtt
     7201 tacagatgtg ataataatgt tgtagacaat atggtaaata tactacagtt tgaaaatgga
     7261 gtgacggcta cttttaattt atgtgcattt actaaagaaa atggaagaac tataaagtta
     7321 atgtttagcc atggtgaagt tggtggggat ttaaataaaa atgagataag aattaaagaa
     7381 tttggaaaaa acgaagaaat agttatgaat ccatcaaatc aacagaatat ggtagaggaa
     7441 tatgatagaa atcttatagc tgaatttatt aaattagttt caaataaaga actagaaaag
     7501 ggtagagtag cagcaaaaga agctatacaa agtcatgtaa tggcatttgc agcagaatat
     7561 tctagggtca gtgatgaagt agtgtatata gaagaatttt ttgatagtgc taagcaaatg
     7621 actaaggaga tagaagaaac tatattttaa ttaataaaaa tttattaaat aaaataaaga
     7681 tagattaaat aatagaaata cgaaaatatg tgttctatta aggaatctat cttttttatt
     7741 aaggtttttt cttatcccta tcaaaaaaat ccatacattc tgataaaaga taagaaagta
     7801 aagcgaataa gatttctgta cttaaaggaa tcgttactga atccataaag gtatcaatta
     7861 tgttcatagc tagaaaagtt agtataaaat caattataaa ttcgcaaagt ttatatattg
     7921 agtatggtaa tttgtttaca tatctgaata tatctaaaat agtagtgcat agaaaatcaa
     7981 ttggagtggt tatacaaaaa tatattaaaa agaaaattaa tacagctcca agagatttat
     8041 attcaaatcc taagaatgat aagaagccaa ttcctataac cgtgaaaata caaaaagtta
     8101 taccaaaaaa aacagcaaaa aataaaaata cagaaataaa ttcgcctaaa ggaatccttc
     8161 ttttattact tatcttaatc aactccttaa tttatgatat gaaatttata taaataataa
     8221 cattcttaaa aattcataat agtcctctgt ttcaaatagt actgtttgag aatctatttt
     8281 tttcatattt ggataaaaag aggccttaaa agctttataa tgttctctaa attttatttc
     8341 tatcttaaat ttatctggca attttattaa gtgcctagat aaatctccag ataaagcttt
     8401 tttgacacct ttttcaattt tttttagtgc aaggtttgga tggattgaaa ttgaaccatt
     8461 tccaattcct tttgacacag gaacagtgac tatatttgga tttagttttt ttgcactctc
     8521 acaaagcatt tcatctccac ttaaaaatgc tacaggaaca ttataatatg cagcaatata
     8581 agcatttata gtaaattcag atgctatttc atcatttatt ttaaaatagt cataatcaat
     8641 atccatagta tgagaaagtg gattaccatt acaagtagct ccagagtgat agccggtgaa
     8701 tatacatgca tcaaagcttt catcaattcc tgccatcata actagtgggt ctttggtcca
     8761 tcctctaagt attttgacgt tttcaggtaa aatatttgga ttaatatttc taccactgtc
     8821 atgtgcatct ttaacaagta tttcatgtac accagattga gtagctccat cacatgctgc
     8881 tgttatctct ttactcattt gcgttctata gtgttgcgaa aattcacatt caagttttgt
     8941 ttcatcccaa tttacaatac cacaagtacc ttctatatca gcacttaaat acaatttcat
     9001 aatatatacc cccttatgtt tattattgat gataataaca gataacagaa aatacataat
     9061 caataaaggg cataaaaata ttatgtctta ctattaacta ttattaaatc tgtaactgaa
     9121 aaataactaa attggtataa aaaaatataa cactatgtgt catttattta tatagttttt
     9181 tatatttgat gtttatataa aattgtaaca tataagtggt tacatttact agtttaacat
     9241 agttaaaata gttaggtatt atttttaagt taaattgatt attataaaat cgcagaataa
     9301 aagaaaaatt taatttatta aaatgcatga aatgtttttt cctcaacata atgctaaatt
     9361 aaactcaaaa agaaataata gaaaaatctt atgtagctta gaaagaatgt gaatgattta
     9421 agggaaaaat agaaatttat tatataatat taggttgtat tatataatac agcctaatat
     9481 tatatgtata tgtgacataa aatcttggta agtgtaaata aaataaatgt ggaaaacaag
     9541 ttaatgaaat caagaaaaaa gtaatttaaa attcaatttc atcttcccaa ttaaattctc
     9601 ctgatttttc tacactctta ggccagtgac tacaccattt aggaacggac ggattttcaa
     9661 ccctatcaac gcttggagta tatggcttta agtcaataac aggactatta tcattagcat
     9721 caatatatgg tatttttatt atgccatttt catggtcgat attgataatt tgtacagcag
     9781 tcaatgctat tggattgggt cttataggtg accttgttgc aaatattcct ataacgtctg
     9841 gtccactttt ataaggtttt ggggtttcta aaatacttct tagttcagga ttatcaaacc
     9901 catcagccca ccaaaataca tttaaatgac tgaaatcgtc gagttcattt aatgcaggta
     9961 catacttttt gtctaattta ataaagacct cttcgccttg aatacatatt ttacctatag
    10021 gatttattaa tatttctttc ataatattac ctccaactat ttaatttatc atttgtaatt
    10081 ttagggtata acatgacatc atgtgagggt caatataaaa taataaataa aaatattaat
    10141 agttagaatg ataatacatt ttttacatgg agcatgacac cagtaataag gcaatcttcg
    10201 tcaatatcaa aagtagaggt atgcaaaggg gaagtaatgt ttttttcacg atttgcacaa
    10261 ccaaggtggt aaaaagctcc ttcacagtgt cttagaaaat acgaaaagtc ttcagcacca
    10321 agagtgggcg agtctttaag gataatattt tcttcaccaa acaattctac agcattttgt
    10381 ttgacgtaat ctactagatg gtttgaattg ataagagctg gataaccttc ctcaatttca
    10441 acagaaatac ttcctccaaa tgttttacaa gtaaaatcgc aaatttctct aattcgtttt
    10501 tttgaaaact ctctagtttc aggagtcaag gttctcaaag taccccttat aaccactttg
    10561 tcacaaacta tattttcttt gattccacct tcaatctttc ctagagaaat aaccacagaa
    10621 tcagttggat ttgtgtttcg actaacgatt gtctgtagag ctgtaataat atgagcagat
    10681 ataacaatag catcaatacc ttgatgtgga taagctccat gacattttga accatgtaca
    10741 gttaattgaa gtgtattggt ggaagcattt agagtgttgt attttaattc aattagattt
    10801 ttgttgatat tgggatttac atggagtcca aatatatatt ttacatttgg tttttcaaga
    10861 catccatctt ctatcataag ttgagcccca ccaatagttt cctcagcagg ttgaaaaaag
    10921 aattttacat ttacatttaa gtaatccttt aatttaaata gtatattaca agtacccagt
    10981 aaaatagcag tgtgtgcatc atgaccacat gcatgcattt tgccaatatt aatagattta
    11041 taagaataat taagttcttc tgttataggt aaggcatcta tatcagctct tatagcaact
    11101 gtttcaaagt ttggactcac gttaatgtat gcagttatcc ctgtatgatt cttatattca
    11161 atataattta tacctatttc atttaagtat ttaataattt ttttcttagt ttgaaattct
    11221 tctaatccaa gttctggagt cttatgtaaa tctcttctta tatttataac ccaatcattt
    11281 atctcatgac aaagagtttt tatattaagc ataaaacaca tcctaactat attaaaaatt
    11341 tatttttctt cattaattat attattttat gtaaatcatt attatgtatc aagtacaaat
    11401 aaaatatctt ataattatat tagagatatt tagtatggag aagaatatat cattttataa
    11461 gaagaggtgt ttaaatgtta ggaataattg cgccatctgg gccattaaga aatacaagtt
    11521 tggaggaaat aaagtttaat ttagaaagtt atggttacga agttaagttt tctgaaagtt
    11581 gtagtttaaa ttataaaggt tatttagctg gtaatgatga tattagagca agggatatag
    11641 aagatatgtt tttagataaa gatgtagata taattatgtg cttaagagga ggttatggaa
    11701 caacaagaat attggataaa ataaattatg atataataaa gcaaaatcca aaaccattta
    11761 taggtttttc tgatataaca ggtttaaatt tagctttcta taaaaattgt ggtcttttac
    11821 catatcatgg aataatggca gcaaatgtag gtaaatgggg taaatttacc tataaatcat
    11881 tggtaaatgc tttagaattt aaagatgaat tatatttaga aaatccaaaa gaagaaaaga
    11941 tttatactgt atgtgaaggt aaagcagagg gaataataat gggaggaaat ttatccttaa
    12001 taattgccac aatgggaaca aaatatgaaa tagatgcaaa agataaaatt ttgtttattg
    12061 aggaaattgg agaacctcaa tataaactgg atagaatgtt aacacaacta tattcttcag
    12121 gaaaacttga ggaatgtaat ggtataattt ttggagattt taaggattgt attgaagaaa
    12181 atgatttgat ggaattacta atagaatttg caaataaggt caataaacct tctatatata
    12241 atcttcagtc aggacattgt atcccaatga taactattcc tttgggcaga atgtgcgaat
    12301 tagatgctac agaaaaaatc gttaaattaa aaaaatagtg tgtatataaa tataatagaa
    12361 atatggtata atcttataca agtaaaaatt tagctaggag gatgaaaaat gtctgatgat
    12421 agaataatag actttaacga actaaagaac aaagttaaag attctgatgt agataaattt
    12481 gagcaataca tatataactt atatttttct gttatggatg gaaagatgtc tatggctgag
    12541 ttttcaagaa aaatattcga ttatatgaga gataacaaca tatctcaaga aaaatttatg
    12601 aaaatacaaa agcaatttat ggagagatat ggtatggaca ctgaagaagt agaaaaacaa
    12661 cttagaaact ttggaatcga cccttctaca gcaggtttta tgagtaataa tacttccagc
    12721 aaagtatcta cagaagattt agagtcattt aagaagagtg caggatttta tgagaaatat
    12781 ggagaaaaaa tccaaccaaa gagttgtata acaacattta taaaaaatga cttaaatgat
    12841 attaatgtaa taatcgacca agaaaagata atgctttgta gtgatagaaa aataaatctt
    12901 atggattcag aactaaatga gtttttatta gaatataaga atatgtttaa taaaaaaata
    12961 aaggtagtaa tgtgcgaaac tacgaataag tatgattatt aaaaaatgaa tttaaatact
    13021 gaagtagtat aaattaattg tactacttaa aaataaaaat cgcaatttat tattccttat
    13081 aaatttgtat aaggaatatc taaactgcga ttttttatat tgtgaagatt agtaataata
    13141 gatagtgttt taaattgtaa tcttctcact aaaactgtgt ttttctatta gaaattttat
    13201 taaaaataaa tatctgataa ttgtattata tgggatgtaa agtattagaa gcttaacgaa
    13261 tctcttttaa tacattaact ccattttgaa tcatactgtt gaaaaatact aagtttaaaa
    13321 tctcagcagg gtgaacgtta ggaatatcat aagtatctac taaattcata ggtacaacta
    13381 tatttttttc aatattatgt tgattaaaat atgattttag agttattgca aattgatata
    13441 tacatatatc tgtgcaatcg ccaactataa ttatattatc tatattatca aaatccaaat
    13501 tttctaatgc aaaaaatcca ttagtagaat ttttaggaag aatttgtagg ttttctatcg
    13561 acttaagttc atcaaccaat tcactttcta catcattttc caaacagtga acaggataac
    13621 tcaaaagttc tattgattta ggagtatgag aatcagtaaa tgctattact ctatttaatt
    13681 tgtttgatat tttttttgta aacatttcaa ttggctttat taaagactct actctaggag
    13741 agtataatgc accttgtctt gcaaagccat tattaacatc aattataaat aaagcagttt
    13801 tactcaaatc ataattttct attttttcta ttggtagatt atctaaatta ctctttaaag
    13861 cttcaagttc atttaataag ttatccatag ttatacctcc aagataaatt taataaaatt
    13921 gtacatatta attatataaa agtcaaggag tacaaactat tgtataatta gctgtattgt
    13981 tttgagtgta aaagatttga agcaatatct ggatagttag taaaaatacc atctacattg
    14041 agttttagac agtgtagtat atcaccctct tcatttacag tatatacatt gacaccaagt
    14101 agatttttgt gacaaagttc aattagagat tcatttacta aaactagagg tggatgaaga
    14161 taactattac ccagaaattt agcaaaattg actacattgt ttataggtaa ataacataat
    14221 aatcctgttt ttatactagg gtctaattta tgaaagtttt ttaaactatt gctgttgaaa
    14281 ctagatataa gaactctatt tttcatatta tactttaaaa taagctctaa aacgtctttt
    14341 tctatattgg gatagtttat tttgtcagtt tttagttcta tatttaaaat aaggtctgta
    14401 ggtgaaatta gaattaatac ttcttctaaa gttggaattt tacattcttt attgctttga
    14461 tatccttcaa aagatgagtt tagagttttt aattttctta aagtaaagtc ttttacaaaa
    14521 ccatgtccat taaaagttct atcaaccttt tcatcatgaa taactacaat ttttccatcc
    14581 ttagttctgt ggacatcaag ttctattcca tatatatcca tatccaaaca ttttttaaaa
    14641 gctagaatag tattttctgg atactttcca ctaaaccccc tatgagcata aatattcatt
    14701 tatatggttc cctcctataa gtataactta tcttataaaa atttaattaa tataatatat
    14761 catattccaa aaggtattgt tatatattag ttaaataact at
//
LOCUS       DAMKMI010000104        14257 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMKMI010000104 DAMKMI010000000
VERSION     DAMKMI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.108"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            127..1755
                     /gene="aspD"
                     /locus_tag="PC429_002726"
     CDS             127..1755
                     /gene="aspD"
                     /locus_tag="PC429_002726"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF3848221.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1876..2871
                     /locus_tag="PC429_002727"
     CDS             1876..2871
                     /locus_tag="PC429_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF3848222.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3060..3443)
                     /locus_tag="PC429_002728"
                     /pseudo
     CDS             complement(3060..3443)
                     /locus_tag="PC429_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3669..3766
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3908..4265
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3908..3936
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4551..4709)
                     /locus_tag="PC429_002729"
     CDS             complement(4551..4709)
                     /locus_tag="PC429_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848223.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5068..6438)
                     /locus_tag="PC429_002730"
     CDS             complement(5068..6438)
                     /locus_tag="PC429_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF3848224.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6721..6819
                     /locus_tag="PC429_002731"
                     /pseudo
     CDS             6721..6819
                     /locus_tag="PC429_002731"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            6838..7259
                     /locus_tag="PC429_002732"
                     /pseudo
     CDS             6838..7259
                     /locus_tag="PC429_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7490..7762
                     /locus_tag="PC429_002733"
     CDS             7490..7762
                     /locus_tag="PC429_002733"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3848225.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8080..8748)
                     /locus_tag="PC429_002734"
     CDS             complement(8080..8748)
                     /locus_tag="PC429_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF3848226.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9584..12004)
                     /locus_tag="PC429_002735"
     CDS             complement(9584..12004)
                     /locus_tag="PC429_002735"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF3848227.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12358..12708)
                     /gene="rsfS"
                     /locus_tag="PC429_002736"
     CDS             complement(12358..12708)
                     /gene="rsfS"
                     /locus_tag="PC429_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF3848228.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(12825..13397)
                     /gene="yqeK"
                     /locus_tag="PC429_002737"
     CDS             complement(12825..13397)
                     /gene="yqeK"
                     /locus_tag="PC429_002737"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF3848229.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13398..14087)
                     /locus_tag="PC429_002738"
     CDS             complement(13398..14087)
                     /locus_tag="PC429_002738"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF3848230.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4814 a         2306 c         1807 g         5330 t
ORIGIN      
        1 agttattatt ttaatttcat actttacttt ttctataaac ctattataat taaagtataa
       61 tcatttttgc aacttgttta ttttattata aaaatgtgaa acttattaca taaggaggaa
      121 tttaacatga aaaatcatac ttataaagaa atcaaaaaca tatatggaaa aataagccct
      181 tttgaattta aggacaaatt aatagatatt gcaaaataca ctgcaaaaga aaataacaga
      241 gaattacttg atgcaggtcg tggaaatcca aactggactt gttcaactgc tagagaagcc
      301 ttttttactt ttggtcattt cgctattact gaaactcgtt ctaattggga tttaggtcat
      361 ttggctggaa tgcctcaaaa aaagggtatt aaagaaagat tttttaaatt tataaatgaa
      421 aatatagata tgcctggagc atatttagct agagatatta taaactttgg tataaatgaa
      481 cttggatttg atggagatga atttgttcat gaactagctg atggaattat tggtgataac
      541 tatccacttc cagatagaat gttacctcat atggaaaaaa tagtacacga ttacctagtt
      601 caagaaatga aatatgatat atcaggtaaa tatggtgatg tagaaatttt tgctgttgaa
      661 ggtgctacag ctgctatgtg ctatatattt gattcattaa tggcaaatga acttctaaaa
      721 aaaggagata ccatagcctt aatgactcca atatttacac cttatcttga aatacctaac
      781 cttcctcgtt acgactttaa agtggtaaat ataaatgcta atgaagttga tgaaaaaggt
      841 gctcatacat ggcaatatac aaaaaaagaa ttagagaaac ttcgtgacaa atctattaaa
      901 gctttattcg ttgttaatcc taataatcct gcttctatag ctatggatga aacatcttgt
      961 aataatctaa tagatgtaat tgaaaattat aataaagatt taatgataat atcagatgat
     1021 gtatatggaa cttttgtaga agatttttca tccttaatgt caaaactacc ttacaacact
     1081 gttggagtat attcttattc aaaatatttt ggagttacag gatggagact tggtacattt
     1141 gcccttcata agaaaaatgt ttttgataag aaaataaatg atttaactgg agaattaaaa
     1201 aaatcagtag ataagcgtta cagtgatatg agtcttaacc cttctagtct ttcctttatg
     1261 gaaagagttg ttgctgatag cagacttgtt gctctcaacc atactgctgg tctttctact
     1321 ccacagcaag tacaaatggc gtttttctct gcttttgcat tgattgacaa agtagatgcc
     1381 tacaaaaaac ttaatatgag tatctgccat agacgtcaaa aactattatt tgaagcttta
     1441 gagcttccaa ttaatgaaaa caaaaacaat gctgcatatt acactcaatt tgatattgaa
     1501 gaatgggcta aattaaacta cggagaagac ttatttaaat ttataagtaa aactgcatct
     1561 ccagtagatg ttctttataa attagctaat gactattcag tagttttact tagtggaaat
     1621 ggattctatg gccctgaatg gtcaattaga atatctctag caaatctata tgatgaagct
     1681 tatactaaga taggaaaagc cataagagag atattaaatg gttatatcgt tgaatggcaa
     1741 aaaattaaga aataattata ttacactgaa atatctaaat tatatgaata aaagagttta
     1801 tgtagacctt aactactttt tttgatataa tcctatcgga attataaatt ttatttatga
     1861 aaggcagaga atagtatgag tattaagaaa aaagttgcca tagtttttac tggtggaact
     1921 atatctatga cagtagatga taaagtcgga gcagctatac caactttatc aggtgaacaa
     1981 atactatcaa tggttaccaa cattgataaa gttgcagatg ttgaagtttt taattttgat
     2041 gaaattccag gacctcacat aacaccttat agaatgatgg aattgaaaaa ttatgtaaat
     2101 gatttattag caagaaaaga tattacaggt gttgtaataa cacatggtac agatagttta
     2161 gaggaaacag cttattttct agaccttaca attgacaaca tcaaacctgt aatagtcaca
     2221 ggtgctatga gaagtagttc tgaacttgga tatgatggtt caagtaattt atctgcatca
     2281 gtttgtactg cagtatccaa agatgctatg ggaaaaggag tccttgtagt tttaaacaat
     2341 gaagtattat tagcttctga agctactaaa acaaatactc tttctctaaa tacatttaaa
     2401 tctctaacta gtggcccatt aggaataatt gactgcaacg aacttgtcct tacaagagat
     2461 attgtaaata gaacaattat agatactgat aaggtagaat ctaaagttgc cttatttaaa
     2521 gcatatgtag gagaaaatgc tgactttata agatttgctg ttgatagtgg ttataaaggt
     2581 atagttattg aggcaatggg cagaggtaat attcctcctc aaatgctttc tggagttgaa
     2641 tatgctagag aaaaaggact tcctgtagtt atagtttcta gatgtcattc aggtagagta
     2701 ttcgatagtt atggttactt tggttctggt agagatttaa aaaatcttgg ttgtatattt
     2761 ggtggggact taccaggtca aaaagctaga ataaaactta tcttagcact tggaaaaact
     2821 gataaccttg atgaaatcaa agacttcttt gaaaaaggaa tctattgtta attctaataa
     2881 cttctattta attatttatt gatatttcaa catttttaaa tcaaaatcta atacttatgt
     2941 cagttaaatg tcagcaaaat attttatcca caatttttac acaaattttc cacaacttat
     3001 ccccaatatt atccacaaga aaaaaggtag cacttatatt agtagctacc ttttatttat
     3061 taatcttcat ctttccagct ttttatccaa tttcctattt ctattatttt ttcttcagtt
     3121 atttcttctc catcatgtaa ttttgaaatt agttctgata atggattgtt atgtagacct
     3181 ccaaaaattc tttttccctc tattttgaga tattctttct cttttattaa tactctatag
     3241 tgtacatgtt tcccaattct ctgcgatatg aaaaaacctt ttttagttaa tctagctaaa
     3301 agtgtaattg tagttgtatg cttccaagca tatgtttttt ctatttaatc tgcaacatct
     3361 tttgatatta cagttttata accagtattc catatgtatt tcataatttt taattctgca
     3421 tctggtaatt ttttattcaa cataatatat tccttctttc aatttatgtc acttcctcaa
     3481 acttatctag cttaaattaa taataggagc tttttatcac aaaaactaca ggtaaatttt
     3541 tctatatatt ctacgtattt agtctgtttt taagtgtgaa attacgttca tacctataaa
     3601 tttattcaat tttcaaacaa tcttgttgaa taataactaa ttctattgta ttatttagtt
     3661 atagaataaa actaaatcgg caaaactaga gaaatctagt gacgcaaagc tatagggact
     3721 aagacttata taaatatctt atgagttatg ttagccagtt gccaaagaga tattgttctt
     3781 tttgttttta tgaaagtttt ttagggggat aataatttat atgtttagag ataaaatgga
     3841 taaatgtaca catatattga ctgcttatat tagcaattcg tatgattatt gtaatttttt
     3901 ggatacaatt tacattccat atagttaata taaaactcaa ttaaattatg accttggttt
     3961 agatagttca ttatttacat tccatatagt taatataaaa ccagaaggaa tatctggagt
     4021 tattggaggg tctattggat ttacattcca tatagttaat ataaaacatt tactaagtgt
     4081 agatataatt ataaatacga tttaatttac attccatata gttaatataa aacctgtaga
     4141 cttcaaccct acaatgcttg ttggaagtgt atttacattc catatagtta atataaaact
     4201 tttgtaaaag tttagaagtt tatatataaa aaaaatattt acattccata tagttaatat
     4261 aaaaccccaa aataaattga gtatttccaa tgcctacaca tatacaaatc tctcaaattt
     4321 gcagtgaacc atgagtagtg caattgataa cacttatcac aagtcctcaa tgccttatat
     4381 ttcaactgtt aagccttaat ttcttcaaaa aatcgaacac tgcaaaattt ctatgacttt
     4441 attatatcat aaaaatatta tttttgaata tctgtaccaa tttgtgatat aataaaagca
     4501 agaagaacta caatctattt tgctgtagag tggagttcat aatttagaaa ttacttagtt
     4561 ttatggaatc tgatttttaa atcaaattcc caaccactct tagttgccgc tttgagtggt
     4621 tttttacgct ttctaaatag tttgctagct atataaacta ttaagctagc agatagactt
     4681 gctagtatgc cttgtaaaaa attatccatg tatattcacc tcccttctta gcgtcgggag
     4741 gataattttt tgatacatga actccactct ataaattgta gattacatct tcttgctaaa
     4801 aatattataa catataattc ttacatattt tacctattta ttacttttta cattataata
     4861 atattctttt aatcaaaaaa acacctacta ttaaagtaaa tgtttttaag tatttttaat
     4921 tttaaaatcc atatagttca tatgaaacta aatataataa ctcaacatat gagcaatatc
     4981 gagacattta cataccacat agttaatata aaacgaaaat aaaaaaatta aacttataaa
     5041 acccttatta atttacatac ccactagtta tttatattca aaaaaatctt ctccatctgg
     5101 gttataaaat aatattctac ttgccaatcc ttgttcatat ttataatttt ctttatcacc
     5161 tacataagaa aatgttaatg tcatgcttct tgatggatct aatgaaatta tatctaaata
     5221 aatagtacat ttttctcctg gctttaatac accgaaacta ttaaaatcca tttttatttt
     5281 agcatattgc cccatatata atgttgaatt agcttctcca taaactttta attttgttac
     5341 atctaaattt tgtgtacttg tattttctat atttatagga actgttgggt ctttatattt
     5401 ttcagtataa tattcaaggt tatcatcata acattcaaga taacttcctt tatcttgcca
     5461 aattatgtgc tttgaatcaa aatcaatctt taatccacat attatatttt tatttgaaga
     5521 cggaagacta atattattga tggtattttc acactctagc ataattttat cacttagatt
     5581 tcctatccca gtcacttttg cagcattttt aagtatatct ttattacttc caatatcaac
     5641 tagtacaata ggattatttt tagccattgg tgaagcaact aaagcataaa ctaaatcagt
     5701 accactagct atataaaatt cttttgcgcc attatagaac ttatttacta tttttttatt
     5761 agtatcatat ctatctactc cacctaatct agttgaatta gtatcattaa ctagtttgtc
     5821 actcattgat gaagtaccac ctattacata actttctaca ccagttgtat taaatggaac
     5881 actttttcca tcagttaata ctatagctgc tttatctcta actgctacag gtgcagcact
     5941 cattgcatca ggctctcctt taaaagcatt agtcaatata actttattta ctttgttaat
     6001 tgagtttatt tcttttgcaa cattatagct tgtcttaatt ctatcactac cttgaagtct
     6061 tttagcctct attcctttat cttttagaag agtttctgtt gctttatcaa tagaactttc
     6121 tccacctatt atgtatacct ttttagcttt ctctactctt ttaagagttg cattaggtat
     6181 gttatttttc ttagttaaaa aaattggtgc atttgttgct cctgcaagtc cactcgcact
     6241 taatccatca gccattgtgc tgtctgcatt aattaatata gctgtagtat aattttgttc
     6301 atcagctata agagcagccg tttcatattt atcttttcct tgaatagtat aaattgtatc
     6361 aagtgcatta gctgatatag gaccagcagc aaacattgac acggctaacc ctagtgataa
     6421 taatttcttt aatttcacaa tatctccccc ttttatatat gaaatactat atatcttaca
     6481 taattctaca ttaaattttc ttttcctcct ttaacgatat ttatcttcct gttaatttaa
     6541 gtgtatacaa atacgtgtta taatgtaact gtaaagaatt aaaaagataa tgacataatt
     6601 agagcagtcc aactgttctt ttataaaact aacttataaa gggagcgtac atatatgaat
     6661 atcaaatcag cttttataag aaaaagatgg gaaaaattct atgtatatgt ggaataagaa
     6721 actggcaaaa agaaacaaaa aagttatgga agctataaaa agaaaaagga tgctgaaaaa
     6781 catttaattg aaataaagtc tactataaat aataataagt ttgttgctcc aaaaggtatg
     6841 acatttgtag aaagatgtta taaatatata gacatgaata aaaataattg gtctccatat
     6901 actgttacaa atacaaaatc ttgggttaaa aattacatag aacctttttt aagaatctaa
     6961 aactcacaga agtaaatcct agttcagtac aagcttttgt aaattataac tttaataatt
     7021 ctacctttac tagtgctaaa gtaaggtata gctttctaag ttctgtaata agggaagcgt
     7081 atagattaag agaaatacaa gaaaattcat gtgattttat aaaacttcct tctaaagaaa
     7141 aatattctac aaatgaaata tacaatagag aagaagtatt attgctaata gaaaagttac
     7201 ttggtaataa tatagaaata cctattcttt taatgttact tctaggttta agaatgtgag
     7261 aagctattgg gcttaggtgg tcagatgttg acttagataa caatctgata aatgttaatc
     7321 aaattcttgt ctattctaat ggcaaaatat cttttaaaga gccaaaaaca ccaaaatcaa
     7381 aaagagcttt atcagctcca aaagaattaa ttgaaaaatt gaaaattgaa aaactaagac
     7441 aaagcaaatt aaaattgtaa ggtatcctgg aaaacagaaa taacttagtt tgtctaaaca
     7501 ccaatttaaa tccgtttcta ccttcttcac taagaaaaac gtttcacaat tttacaaaaa
     7561 aaataattta aaaaagatta gaatacatga tttaagatat acaagtgcca ctctgctgct
     7621 tttaggggga actaatatga aagttgtttc tgaaagattg ggtcatacag atataaaaat
     7681 aactatgaat agatattctc atgtacttaa agaaatggac aaagaagctt ccgacaatct
     7741 aagcgaactt ctatttaaat agtaaaaact gactgtcagt taaatgtcag ttaaacctaa
     7801 aaaaataggt tttgtcagtt gattatcagt caattatttt ataatagttt cacttaaaaa
     7861 cgtctatatc tacacatttt actatttgtt tatgcttatt aatatctatc tgttattatt
     7921 aattctaata gcttctattt aattatttag ttcagtttac aggaaactac acacaaacca
     7981 taaaatagga tgttgacaat ataacagata gtatgctcct gtaaaaggta acatagtatc
     8041 tgttatattg tctggattcc caaattagct atttggaact taactaaaaa atcctaatat
     8101 aagggcaact attataatgg caatcggaat acatactgtg taatcctgtt ccatagagtc
     8161 atcatctgaa tgtagtttta caattcgcaa tttagcaatc agtttgaagc agaaatagcc
     8221 tattattgtt ccaattacgt tcgtgagtaa gtcgtctata tcggttgctc tgtgtaacgt
     8281 aaaaagctga atagtttcga taaaaagaga taatccaaaa ccaatcaaga atgtatttct
     8341 caaacgttca aaggttttgc ttataagcgg acataaaaaa cctaatggga caaaaagtaa
     8401 aatattcaaa ataaaactca aactaaatcc atcactaaaa ggaataagat ttatgtttgg
     8461 attaaagaac gtttccccta actgtgacag tcttatatat tcaccgaatg taggtatacc
     8521 aacaatatgc gatagtgtta aacataagta gtaatacatt gccagtgata aaatagatgt
     8581 tttgaccttt ggaatatcac tatttttctt gaatattaca actaagaaaa taccaatcaa
     8641 gataattccc aacaagaaaa taggattaat taacaataaa tctttgatag cttcaaacag
     8701 actgagttga atttctacac ctgattgatt atttgttaca gtctccattt gtacatacct
     8761 cctcataaca tttcaattat ttctaaaaaa caaaatagaa gataaatctt aaaatcatcc
     8821 tatcaaaatt tttaaatttg cctttatcag tttataccac cagttaattc ctgtcttact
     8881 tctgtatggt attcttggga ttctttcatc aaattagaca aaaaatggtt accatatcaa
     8941 taaactggtt acgctccata tctcccgaag caaccatccc ctgagaataa atccgtatca
     9001 ggaatctcca catctactgc acctatcaca agttatttga taacatcctc agacaatccc
     9061 aacttggata tatcgacaac acccattaga ataccactgc tgaccatact tgttttcctg
     9121 ctgataattt taatcaactg ggaagttgaa acgctatctt ttttacaagt tcctttttag
     9181 aaacatggta gctattatat gaatctgaat ttcgttgtct gggtgtttca tataaagcat
     9241 agatatgcac cttttttgcc tgttttcatt atatagaaaa aaatttcaaa atacaataat
     9301 tatatttttt tcgtttaagc ttagagcagt tccttgacga acaacacttt ggaaactgaa
     9361 tgatttacat accacttaat acattaactc gatttaacat taattataat aatatttaaa
     9421 acaacaacta gggtatataa gaatgaactt tttagttctt taatcatttt tttgtataaa
     9481 atcaaatcta tttttataat ttcaacaatg aaaaaaggct tatctctatt ttgagacagc
     9541 ctcatttatt ttataaattt atatttttgt tcatattttt gagttattta acaactatat
     9601 ttactaattt tttaggtaca gctacaactt taactatagt tttaccttca actaatcctt
     9661 ttattttttc atcttctaaa gctacctttt ccatctcatc tttagatata tttgagttaa
     9721 cagttaattt tcctctaact tttccattaa cttgtacaac aacttctatt tcatctttta
     9781 ctaaagcttt ttcgtcatat ttaggccagt ctatatcaaa tacacttcct tctttaccta
     9841 tcatagtcca tagttcttca cctaaatgag gggcaaatgg tgcaattata gttactatag
     9901 tttgtacacc ttcttttata actgcttcgt ttatattatc taactcttta tatttataca
     9961 tatcatttat taattccata agagctgata tagcagtatt aaatccgaat ttttcactta
    10021 aatcagcagt aactttttta agtgtagagt gtatagtata tctcatatcc ttatcttggg
    10081 aatttaattc tccaaactct acatcctttt taaccacatc tgctaactcg tcaaccaatc
    10141 tataaaccct atttaagaat ctaaagcatc catcaacacc ttgctctgac cagtccaaat
    10201 ctctttctgg tggtgctgca aataatacga aaagtcttgc agtatctgct ccatactcat
    10261 ctattatatc cataggagat actgtattcc ctttagactt actcatttta gaaccatcca
    10321 taagtaccat tccttgagta agtaagtttt taaatggctc attgaagtct accattccca
    10381 tactcttaaa tgcctttaca aaccatcttg cataaagtaa atgcattata gcatgttcta
    10441 ctccacctat atattggtct actggatgcc atctattaac taattcctta ctaaatggtt
    10501 cattttcatt attgctatcc acatatctta agaaatacca agaagaatca acaaatgtat
    10561 ccatagtatc aacttccctt ctagctggtt ttccacagtg aggacaagtt gttgacataa
    10621 attgctttga agtcgtaagt ggagattccc cttttccagt aaattcaaca tctgttggta
    10681 aaagaactgg taaatcttcc tttttaactg gaactattcc acattcatca caataaacca
    10741 caggtatagg acaaccccag tatctttgtc ttgaaactaa ccagtctcta agtctataat
    10801 taattgttct ctttcctcta ccttcttttt ctaacttatc gattattcct tcaaatcctt
    10861 ctgaagcatc taatccatta aattcttctg aatttatcat tttgttatct tctgttatta
    10921 catcaattat gtctaaatta tatttttcag caaaatctct atctctttca tcatgtgctg
    10981 gaactgccat tatagcaccc gttccatact caactaatac atagttagct atccatagag
    11041 gaactttctt gccatttaaa gggtttataa catatctacc tataaacatt ccttcttttt
    11101 ctacatcagc tgctgttctt tctatttctg tcatagtatg cattttttga acaaaagctt
    11161 caacatctgc ttcatattca gtccctgcaa ccaattcttt aactaattca tgctctggag
    11221 caagtaccat ataagtaact ccataagttg tgtctggtct agttgtaaat actgtcatag
    11281 atttatcagt tccatctata tcaaaaacta aatctgctcc tgtactttta cctatccaat
    11341 ttttttgcat tgttttaact ttttctggcc atccatctaa tgtatctaag tcattaagta
    11401 attcttctgc aaactcagta gtcttaaaat accattgttc taaatctttc tttaatactg
    11461 ttgctttaca tctctcacat gctccttgaa caacttgctc atttgcaaga acagtctcac
    11521 aagaaggaca ccagttaaca tatgattttt tcttatatgc aagcccatgc tctaaaaact
    11581 tcaaaaatat ttcttgtgta aatctatagt attctggagt tgaagttgca acttctttat
    11641 cccaatcata actaatccct aataaattta attgctcttt catttcttct atattttcca
    11701 ttgtccattt atgcggatgt attccatgtt ttatagctgc attttcagct ggtaatccaa
    11761 aagaatccca tcccattgga tgaagtacat tatatccttc catttttttg aatcttgcga
    11821 caacatcacc tatagaataa tttctaacat gtcccatgtg tatttttcca gatggataag
    11881 ggaacatttc taatgtgtaa taattaggtt tttcagtttg tgcagtatcc attttatact
    11941 gattattatc tttccagatt ttttgccatt tcgattctac ttctttaaaa ttatatacgc
    12001 tcatattttg cctcctattt ttaattgtgt ataactacct tatttagtat tatttactta
    12061 taatttccaa ataattcttt gaaaataaaa aatcctcgtc actaaactta cgtttaggga
    12121 cgaggataaa tcttcgcggt accaccctaa ttagcctact acatatatag actctcttag
    12181 atagctttta atccagccat ggagaataga ttttaatcta gtaagttcaa agttatataa
    12241 tattagttct caccttccac taactctctg aaaaaatata atcttttact gctctagtct
    12301 attcaattta atataatttt tgaaattttc taattataat aacatatatt atcatgttta
    12361 tgctaatgtg tcaatatcta tataaggtgc atctttccat aatttttcta aattataaaa
    12421 ccctctattt tcctcattaa atacatgaac cattatgtct ccataatcaa ggagtaccca
    12481 agtttgagtt ccttgtcctt ctttacctct tggctctaat cctagttttg ctaattcatc
    12541 ttccacattg tcagcaatag cttttacctg tctctgagaa gatgctgtag taataataaa
    12601 ataatcacac aaactagaaa cttttcctat atttattata actgtatctt gacctagttt
    12661 atcttctatg gcatcatatg ctatttttgt catttgttcc actgtcatat aaaaattcct
    12721 cactttccta attaaaatac acttagttat tgtaacatat taatacgatt ttgtaacata
    12781 gtcaaattta aatttattaa tatgtttgcc tttatatagc ttttttataa taacttttct
    12841 ttaattagat agtttcttgc cataactgtt cttggatgta tctcttcttt tttattgatg
    12901 acaaacatta aagtattatt aaaagaagtt aaaagtgctt catcaagctt ccctccatat
    12961 gctaattctc ttaatgtatc tacaactggg aattttcttc cttcttcaat taaatcagct
    13021 atgtaaatta ttttttctaa aagagacatg tcctctctcc ctgttgtatg atatttgatt
    13081 gcatttataa tatcttcatc ttctacttca aattcatact cgcatatgta cgccccaatt
    13141 acgctatgag acaaggaacg atttccatcc tctattgggt ctaaatatat ttcatactta
    13201 tttacataat cctctatttc cttgtcactt aaatactttg cacaatcatg aatcattcct
    13261 gctaagtaag ctttttcttt gtcacatcca taaatctcac ataatttttc agcacatttt
    13321 gcaacatttt ttgaatggct aagcctcccc actggtagca tttgatttag tctttggtta
    13381 atattttcta agttcattta atcatctcca tattgatata gtttattttc atataaataa
    13441 ttttctacat tttcgggtac taagtaacgt attgacttgc cttcatttag ctgttccctt
    13501 atatatgtgg acgatatatc cagtgatgga acataaactg ttataatgtc agcattatac
    13561 ttcttagtta gtttttctat agtctcttgt gaccttaaca aactaattcc tggtctagtt
    13621 gcggctataa aagtagctaa ctcaaagttt ttctttacat ctttccactc ttcaacacta
    13681 caaattgcat ctgcacctgt tataaaataa atgtctgcat ttttgtattt tttctttaag
    13741 tattttaatg tatctattgt ataagtcctt ttctttcttt cgatttctac ttttgaaact
    13801 aaaaaatctt cgttgctttc agttgctaga agtgtcatat tatatctatc atacttatct
    13861 gtagtgatac ataatttatg tggtggattt cctgatggta taaagattat tttgtcaatg
    13921 tcatacttgt ctctaataaa ttctgctgtt gctaaatgag catagtgtat agggtcaaaa
    13981 gtacctccca atatacctat tttgattttg cctttatggc gattaaattt ctctagtttt
    14041 tcagtatttt tcaactctgc catcttaact agattttcac ttctcatatt ttgtctcctt
    14101 ttaatgttat aatactattg taccattatt ttttatttct taaagcttat aatttgtttt
    14161 tttgggtata taattatatt tatatgctaa aaaacactta aaaaaataat tatttaacat
    14221 gattctactt tactttttaa taaaaataac tttttac
//
LOCUS       DAMKMI010000105        14045 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMKMI010000105 DAMKMI010000000
VERSION     DAMKMI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.109"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            438..1331
                     /locus_tag="PC429_002739"
     CDS             438..1331
                     /locus_tag="PC429_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF3848231.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1331..2725
                     /gene="gltA"
                     /locus_tag="PC429_002740"
     CDS             1331..2725
                     /gene="gltA"
                     /locus_tag="PC429_002740"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF3848232.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3091..5310
                     /locus_tag="PC429_002741"
     CDS             3091..5310
                     /locus_tag="PC429_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3848233.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5457..6203
                     /locus_tag="PC429_002742"
     CDS             5457..6203
                     /locus_tag="PC429_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848234.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6203..8083
                     /locus_tag="PC429_002743"
     CDS             6203..8083
                     /locus_tag="PC429_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848235.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8202..9593
                     /locus_tag="PC429_002744"
     CDS             8202..9593
                     /locus_tag="PC429_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3848236.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9700..10740
                     /locus_tag="PC429_002745"
     CDS             9700..10740
                     /locus_tag="PC429_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF3848237.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11229..11882
                     /locus_tag="PC429_002746"
     CDS             11229..11882
                     /locus_tag="PC429_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF3848238.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            11958..12167
                     /locus_tag="PC429_002747"
     CDS             11958..12167
                     /locus_tag="PC429_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848239.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12307..12873)
                     /locus_tag="PC429_002748"
     CDS             complement(12307..12873)
                     /locus_tag="PC429_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF3848240.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13034..13687
                     /locus_tag="PC429_002749"
     CDS             13034..13687
                     /locus_tag="PC429_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF3848241.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13845..14021)
                     /locus_tag="PC429_002750"
     CDS             complement(13845..14021)
                     /locus_tag="PC429_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848242.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5500 a         1391 c         2373 g         4781 t
ORIGIN      
        1 tttatacaat atgaaagctg gtattgcaat agattaaagc cttatacacg tttagttttt
       61 aaagaaatta gtgttattat gaaaacctaa gttaatttat taaataaata attgaataaa
      121 aaaattttat tatgtattta taaaattttt gttacaaatt tggcataata tagtatactt
      181 taagtgtgta ttaggttaaa tctataacaa attagacgta tgatatagac ttagtgttta
      241 ttggcattta caagaaattt gttaatatca actatttgta gtttgggcag ttataattat
      301 tatagggggg atgtccaata caaaacaatc aaccaagaac taatattata ttataaagag
      361 ttatagtttt gtgtgtttat gtttttaatt aaatttaaag tgggaaaaat atatttgtta
      421 gaatggagaa tagcgagatg agtaataaaa tagttagtaa gagacaatta acagatagta
      481 tttatttaat ggaaatagag gcacctagag ttgcaaaatc ttctcaacca ggtcaattta
      541 taataattaa aaatgatgaa aaaggagaga ggattcctct tacaatagct gactatgata
      601 gagaaaaagg aacagtaact attgtattcc aaacagttgg tgcttcaact aaaaagttag
      661 caatgtttga agaaaacgat tttgtaatgg attttgtagg gccattagga caagcaagtg
      721 aatttataca tgaagatata gaagaattaa gaaataaaaa gatattattt gtagctggtg
      781 gagttggttc tgctccagtt tatccacaag ttaaatggtt taaagagcat ggattagatg
      841 tagatgtaat aattggtgca agaactaaag aattaattat attagaagat gatatgaaaa
      901 aagtagctaa aaatgtatat gtatcaacag atgatggtac atatggattt aatggaagag
      961 ttacagacct attaaaagat ttagttgata atcaaggtaa aaaatacgac caagcaatag
     1021 taattggacc aatgataatg atgaaattta tgtgccaatt aactaaagaa ctaaatatac
     1081 ctactatagt aagtttaaat acaataatga tagatggaac aggtatgtgt ggtggttgta
     1141 gagttagtgt tggaaatgaa actaagtttg catgtgtaga tggtcctgaa tttgatggtc
     1201 atttagttga ttttgatcaa gcaatgagaa gacagtctat gtacaagact caagagggaa
     1261 gagctatgct aaaacttgaa gaaggcgata gtcatcatca tagtaattgt ggatgtggag
     1321 gtaacaaata atggatgcta aaaaagtaaa agtaccagtt agagaacaag aacctgctgt
     1381 gagagcaaca aattttgatg aagtatgctt agggtataat aaagaagaag ctatggcaga
     1441 agctaataga tgtttggctt gtaaaaaacc aaaatgtgtg ggtggatgtc cagtaggaat
     1501 cgacatacca gggtttataa caaaaattaa agaagatgat atagaaggtg ctgctaaagt
     1561 aatagctaag agcagttcat taccagctgt ttgtggtaga gtatgcccac aagaaagtca
     1621 atgtgaaggt gtatgtatac ttggaataaa atcagatgct gtatcaatag gtaaattaga
     1681 aagatttgta gcagattggt caaaagaaaa tgacataaat ttatctgata cagagcctaa
     1741 gaaaaatcaa aaagtagcag taataggaag tggtccagca ggtctagctt gtgctggtga
     1801 tttagctaaa aaaggctatg atgtaacaat ctttgaagca atgcatgagc caggtggagt
     1861 tttaacttac ggaatacctg agtttagact tccaaaacaa gctgttgttc aacctgaaat
     1921 agacaatata agaaaattag gtgtaaaaat agaaactaat gtaattgttg gtaaaacaat
     1981 tacagtagat gaacttatag aagatgaagg atttgaagct atattcatag gttcaggagc
     2041 tggtcttcca atgtttatga atataccagg ggaaaatgca aatggagtat tttctgcaaa
     2101 tgagttttta actagagtaa acttaatgaa ggcttataga gatgattatg atacacctat
     2161 tagttctggt aaaaaagttg ctgtagtagg tggtggaaat gttgctatgg acgctgctag
     2221 aactgcatta agattaggtt cagaatcata tatagtttat agaagaagtg aaaaagagct
     2281 tccagcaaga gctgaagaag ttcatcatgc aaaagaagaa ggcattatat ttaatacatt
     2341 aacaaatcct aaggaaattt tagtagatga aaatggatat gttaaaggta tggtttgtat
     2401 aagaatggaa ttaggagagc cagatgattc tggaagaaga agacctatag aaatagaagg
     2461 ttctgaattt gtacttgatg tagacacagt tataatgtca cttggaacaa gcccaaatcc
     2521 actaatatct tctacaacaa agagtcttga tataaataag aagagatgct taataacaga
     2581 tgaaaatggt cagacttcta aagagggtgt atttgcaggt ggagatgctg ttactggagc
     2641 agcaacagta atatctgcaa tgggtgctgg taaaacagca gcagcatcaa tagatgaata
     2701 tttaaaagca aaagttaatg cttaatttgt aaattaaaga gagggtgtct caaaatgaac
     2761 tttttagttc atgaggcact ctttttgtac aaaatcaaat atgttttaat catttcaaca
     2821 atgaaaattt cattcctgta gaaagtctaa cttttaggtc ttagtacaat ctctattttg
     2881 atgtagccac ttttttagat ggtttaataa tttatcagtt attattgtaa tatttgatac
     2941 taagtaacat aactttgtct aactatgata aaatagttat acaataaagg tcttgtaagg
     3001 agtgaataat gtgtggctaa taaaatcaga tttgtcaaat ctaacattaa ataatagaaa
     3061 aataaattgt gtaattaatg gtattttaat atgaaaaaaa gagaaaaagt atttgatggg
     3121 ctagttttta agcatatatt aatcttatta atactattag ttttgattga tttaacaagt
     3181 accatcatat atgcaagtaa ctctatggaa attacttcta gaaatatgat tgaggcatca
     3241 aaaagagaac ttgaaaacta tttagaagta aacatatctt tattaaaagc tcttagtcaa
     3301 gatgatagat tttctaatgg tgaaacatca ctaattgaaa agggaaaatt attaagtcca
     3361 tatcaaaagg aatataatct ttttatgatt ggtataacag atacaaaagg aaatgcttca
     3421 agtacatata gggaaaatgt taactcaata aaagataagc ctagttttga gaaagcaatt
     3481 aaaacaaagc aagtagtagt ttcagatatt gaagtcagta atgtaacagg tgacaaagta
     3541 tttatcatat atgtaccaat tgtaaaaaat aatgagatga ttgggaccat ttttgcttct
     3601 ttttattttc aagatgtaaa taatttaata agtagaagca attttgatga ttctattaaa
     3661 tttctaatga tagataaaga ttatactatt atttctcatc caaacaaaaa atatgtaaat
     3721 gataaaagta agatgcttga tttagaaggg aatattatag gaaccactaa aagtgaaatc
     3781 ttaaaaaata taaataaaaa atgtcaaggt gattttttat cttgggataa ttggagatta
     3841 tataatgtaa aatatacgaa cataaagtgg actaattgga ctttagtatc aaaatgtaat
     3901 atatttaaga actttaaaaa tctaatagtt aattttatga taaagttgta tttttatata
     3961 gtaatattta tgatattgtg gaaattaagt aatgctaaat taatagaaca acttaaaaaa
     4021 ttggcttatt atgattcttt aagtggaata aaaaataaag aaaagtttag aaaagattct
     4081 atgtatattt taaagaatta ttatcaagat aatttttact tagtacaatt ggatgtcaat
     4141 aaatttaaat atataaatga gatgtttgga tatgctgaag ggaataagat attaattcat
     4201 atatcacagg ttttaaataa taatacaaat aaatatgaaa tttgtgctag gatggataat
     4261 gaccatttta tattgttgat tgcttgtaac actgaagatg aacttttaaa tagattatct
     4321 aaaataaata aagaaatttg taacttaaat actactaact catctaagta taaaattgtt
     4381 atgtcaagtg gaatatataa gattactaaa aaggatgata ttaaaaagat tgacttgtta
     4441 attgatagag caaatattgc agcaaaaagt aaaaaggaaa aatatgaaca ttcatattca
     4501 ttttttaatg aagatacaag aaatcgattg tacaaggaaa aacgattgga agataatatg
     4561 aataaagctt tagaaaaagg tgaatttata gtatattatc aacctaagta tagtttagat
     4621 gatgtaaatg aaattgaagg agcagaagcc ttgataagat ggaatagtcc agaatttggg
     4681 tttatttcac ctatagattt tgtaccttta tttgaaaaga atggttttat tgtaaatatt
     4741 gatatgttcg tgtttgaaga agtatgcaaa acattaaaca agtggataaa taaagggtat
     4801 actcctgtac caatctctgt aaatatgtct agagttcatt tatataggga taattttata
     4861 gaaaatataa ctgatttgat aagtaaatac aatatatctc ctgagtttat tgagcttgaa
     4921 ctaacagaaa gtgttgtgtt tgataattta aatatattga ttgatataat gaaaaagatt
     4981 aaagagatag gattccttat atcaatggat gattttggtt cgggatattc atctttaaat
     5041 ctgttaaaag acctttcttt tgatatttta aaattagata gaggattttt aatcgaaaca
     5101 acagatacaa aacgtggaaa aattattatt tctaagattg tggaaatggc aaaagcgatt
     5161 gatattaaag taatctgtga gggcgtagaa acttatgaac aagtagagtt cttgaaagag
     5221 ataggttgtg ataaggttca aggatactta tttgctaaac ctatggtatt agatgaattt
     5281 gaaaaacact taaactttaa atttgattaa tcattaataa ttattagact ataagatacc
     5341 ctaacaactt acttacaaat atgtaagtta attgttaggg tatttttatg gttaaagtag
     5401 tatattcacg atattatggt attgtagtaa aaataaaaaa ttatggagaa tataaaatga
     5461 acatattgag tattagaaat atatcaaaaa catattgtgg aaatattcca tttaaagcat
     5521 tagataaggt tagtctaaat atagaaaagg gagaatttgt gtctgttatg ggaccatctg
     5581 gaagtggtaa atctactctt ttaaatataa tatctacagt tgatagacag agtgaaggtg
     5641 aggttgtatt agatggatat gatgtaagta aattgaaagg agaaaaactt gctgaattta
     5701 gaaggaaaca acttggtttt gtatttcagg attttaattt gattgacact ttaactgttg
     5761 gcgaaaatat tatgttgcca cttacactag agggggcaag tatcaaagat atgaatatac
     5821 aaactaaaag tatatctaaa tttttaggaa tagataaaat tttagataga aaaacctatg
     5881 aaatttcagg aggtcaagct caaaggtgtg ctattgctag agctattata aataaacctg
     5941 ccatacttct tgcagatgag ccaactggaa atcttgattc aaaatctact gatgatgttt
     6001 tgaaattatt tactaggata aacaaagaac aaaaggtaac aacacttatg gtaactcatg
     6061 aagcatatag tgcaagttat tcagatagag taatttttat aaaagatggt tgtatatata
     6121 cagaaataaa aaaatctgaa agcagtaatt ctttctattc tgatatacta gctgttttgt
     6181 ctcaaatagg aggtgtaaga tagtggattt tagaaagttt gcttataata atatagttag
     6241 aaatttcagg gcatatttag catatttttt aagttctgtt ttttctataa tgatattttt
     6301 tgtttttgca gtgtctatgt tccatccaat tataacaaaa tctggaatgc agagtggttc
     6361 tactgcattt atggctctta tgacttcaga attaataata ttcggtttta gtctactttt
     6421 tatattgtac tctttaggaa atttcttaaa atatagattg aaagattttg gagtattgat
     6481 gataattgga atgagtaaca gacaactgaa aaaattgata ttaattgaaa atttgataat
     6541 aggattttta gcagtgatgt taggaatatt acttggttta tcaatttcaa aattgttttt
     6601 attgtattta agcaaactgt tttatatgaa tttaactgaa ttttattttc cagtaaaagc
     6661 aatgctattg actattgtat catttatgat tttatttttg ataacaggac caatgagctt
     6721 aaaattattg agtaaaaaga gcattttaga gcttttagta ggtacaaaaa agcctaaaaa
     6781 agagataaaa tcttcaaagc tatttggaat aattggtgta atatgtctta tattaggata
     6841 tgctatgata ctatttggca ataagttaaa aataaatgga ggtatgtata caacagcact
     6901 attaattact ttggggatat ttttattttt ttctcaattt actattttga tattaaagta
     6961 tttaaaaaat aaagaaaaat tttataagaa caaagtaaat atgttactta taagtaattt
     7021 gatatataaa ttaaaggata atacacgatt gttattttta ataacaatac ttttatcggc
     7081 tactttagta gcatttacaa caacttcaac acttgtaagt tctcaaggag aagcttctaa
     7141 aaataatttc ccaatggttt actcatattt ttcagagcat aataatgaaa aagaatatga
     7201 agaactacaa caaattagag aagcaatcaa aggatatgat tataaggagt taagttttcc
     7261 tgtacttaaa tataataggg aagtcttact aagtgtaaag aattataaca aattatcaaa
     7321 ggaattggga ttagataaaa ttagttcaaa gttagaaaaa tatgaaggga ttataattcc
     7381 tagtagcaat tcgcaagaac atataaattc tttaaaacaa ttaaaaaatt atagtataaa
     7441 aaatttaaat atagaagtaa aaaaatcaaa aaataaaata atttttccta caggactgtt
     7501 tagtaaagtt gtggttataa gtgatgactt atacaatgaa gtaaaaggtg aattttcaaa
     7561 aattaatttt catggatttg attataaaaa ctggcaaagc agtgcaaaga taactgaaaa
     7621 tttaaaagaa aaacatttta atttggatag agaatataac aggtcatatt ttttaacatt
     7681 accagatatg tatttagtag agttacaaca aagtaaaatt cttcaattta tgggtgtttt
     7741 tataggagtg atattattta tagcagtttt tagtttttta tacttcagat tatatacaga
     7801 cgagatagaa gataatgtaa agtacaaaaa actatcaaaa ataggtctat cttttaaaaa
     7861 tatgaataag attgttagta tagagatagg tacattgttt tttataccgt attttatagc
     7921 aattataaat agcataattt ctttactgtt tttaaatcaa gtattaaata atagttttaa
     7981 gttagaaaat ttgggtatat tactactgat atcatctata tattcacttt atttttactt
     8041 attaaaaaaa aattatacta gaaaagtttg gttttttgat taaagtggta tataatagat
     8101 atataaatta atatttaaaa agtagttaat attaggggag ggcatttgca cctcccttgt
     8161 aatatgtgga aaattgaata ttagaagaag gaggagactt gatggaaaat caacaattat
     8221 taggaacaga acgaattagt aaattactac taaaatattc tataccagct ataataggta
     8281 tgttggtaaa tagtttatat aatgtagtag atagaatttt tattggaaat ataccagggg
     8341 ttggaccact tgcaataaca ggtcttggtg taacaatgcc aataatgaca ataatacttg
     8401 catttgggat gttgataggt ataggaacta ctactaccat atcaataaaa cttgggcaag
     8461 gtaaagtaga agaggcaaga aaacttatag gaaatgcaat gacgttatct gtaataacag
     8521 gaattatcat aatgatactt ggaatattat ttgcaaataa aatacttaca ttgtttggag
     8581 caagtgaaaa tactttaata tatgcaaaat catatatcaa tattatatta ttaggtacag
     8641 tagtaaatct tctttccttt tctttaaacc actcaataag agctgatggt agtcctaaaa
     8701 tttcagcagg aataatgata gtaggatgtt taactaatat agttttagac tggatattaa
     8761 tatttggatt caatttggga atacaaggag ctgcaattgc tacagtaact tctcaggctc
     8821 ttacagctat tttaactata gggtattata taagtggaaa atcaaactta cgattcagta
     8881 aatctaatct aaaattagat aaaaagctaa taaaagcagt gtttgcaata ggtatgtcgc
     8941 catttgctat gcaacttgca gcaagtttag ttcaagtaat atcaaatatt gcacttaaaa
     9001 ctcatggtgg agatttagct ataggggcta tggctactat ttcttctatt gcaatggtat
     9061 ttttgatgcc tatctttgga ataaatcaag gagcgcaacc tattataggt ttcaattatg
     9121 gagctgaaaa atatgatagg gttaaaaaag catatttagg ttcattagta gtagctacta
     9181 taattttatg tatgggtatg gtagtagtta tgcttttccc agaagctatt ataggtatat
     9241 ttaataaaga ccctgaactt atgaatatat ctgttaatgg attaagaata tacttactta
     9301 tgttgccaat tgtaggctta tcagttacag gaacaaactt tatacaatct ataggaaaag
     9361 caaaaatggc tatgttattg agccttttaa gacaagtaat tttattgata ccagcagtat
     9421 taattcttcc tacattctta ggtttacagg gtgtatggac agctcaacca gtgtctgatt
     9481 ttatagctac agtaattaca ggtatagttg tatttagaga attaaaaaga tatactccta
     9541 aaactgaaaa attaaatgaa aatgaaagac taaacgagat aactactgag taaaaatata
     9601 aattcagata attgagcaag aaattgcaaa aaatacgatt atatgataaa ataaaaccgc
     9661 tgtcaaggat aagagaaatt ctaattatgg agtgatttta tggaatttac aaagaaaagc
     9721 aaattaaaaa atatgtatgt aaacatgaat gtagctagaa tgttgtctaa gataaatgag
     9781 tataagggta gacaactgct ttacaaaaaa caaccaaaag aaattttaga aaacttagaa
     9841 aaaaaatctt tagtagattg ttctgaatct acatacattg gaaatcaaag agatagtagt
     9901 aattttaatc tagaaaagtt gataagtaat gaagttacac ctagaagtag agaggaattg
     9961 agtattgttg aatacagaga tgtggtaaaa actattaaca gtgcttatga gagtattccc
    10021 atcagttcac aaacaattct tgagcttcat ggatacttat ataaattttc atctactaga
    10081 ggaggaagtt ataagtcaga taatgatttt attgaacaca atttaaaacc tagcgaattt
    10141 ataagtactg attcaagtgt caataaagtt gaaaaagctg tagaagaaat atgtgaagct
    10201 tataatacat tgatagagga agatgaaatt gatatattaa tattaatttc tgcatttgtg
    10261 ttagatttca tattgataca tccattcaaa gaaggtaata taaaaatggc gagagtactt
    10321 atcctattgt tactaaataa aaatgggtat gaagttggaa gatatataag tttaggaaag
    10381 atatttgatg atagttcata tgaatattat agtaatttaa attatttgaa agcctcaata
    10441 ggtagtgaaa aagcagatat gaatgcatgg attgagtatt ttttagagac gatattaact
    10501 gcatatgaga agctggatga tagtttaaat atatctgata agaaaagaca aacaaaaact
    10561 agtagaatag agaaaataat aaattctacc cttggatatt ttacaaaaga agatataaga
    10621 gatttatgtc ctgacattcc agaacctact ataaatagag tttttaataa tctaagaaaa
    10681 caagataaga tagaagttgt tgcaagagga agaagtgcaa aatggaagaa aaaatattaa
    10741 aaaatgttta gataactaaa atggtatagt gttttacact ataccatttt taatgtcatt
    10801 agcatgagca ataatttaac aacttaagca atatcaattt tatttattga ttatttttga
    10861 actgaaaaat ggcgttatat ggtatacctc agtggtaatt aattttggat gagaagatat
    10921 aatattacag aatatattag gagtatatgt agaaaaaata taataaaagt gtgaggctta
    10981 gtaattaaca aattatgtgg cttcacactt ttttataaaa taaagatact tagcagaata
    11041 atattttagt tgatatgttt ataagatggt acttgtttgg gtaaaagtat aaatagtaaa
    11101 aaagtagaag taatgtgtat aaaaacataa gttacattct gtaataatag ttattttata
    11161 attcaacaaa agaatagaat gggtatattt gtgaaataat aatataaaat aatcttagga
    11221 gtgagcttat gaagaaaaaa ttattatata taaatgtaaa ttcaaaacct gaagacttat
    11281 cttcaagtaa aactgtagca agaaaattta taaataaatt tatggaaaaa aataaagatt
    11341 ttgaggttga agagatagac ctttataaag aacatatacc aagacttgag tatcagtact
    11401 ttgaaaaaag aaatagcata gttagtgaag aaaatgctaa aaatttagac gataaggata
    11461 gaaaagagct tacaaagatt agaaatcttt gtgaccaatt tgtgaatgca agtgtttatg
    11521 taatagcagc acctatgtgg agtttatctt ttccagcacc acttaaagag tatatagatt
    11581 gtataattca agatggaaaa actatatctt ttgaaggcaa tgataaacca caaggaattt
    11641 taaatgatat tgatagaagt atggtatata tacaatcttc tggtggacac ataccatggg
    11701 tgttgaagcc agttatgaat aaagggttaa attatgtgga aagtattatg aaatttatag
    11761 gaattaaaaa atttgatgaa ttgttagtag atggaactgg aactagtgaa gaagaaagac
    11821 aagctgcaat agaaaaagca tctgaaaaaa tagatggtat aatagatggg atgaaatttt
    11881 aattataata ttttattaaa aatataaagg aaaaacctct tttaatgtag aaacttatat
    11941 tgaaggaggc gagaatcatg cgagaagaaa aaagtaatga aaagtatgat tgttattggt
    12001 gtaatcaaga gaataatttc tgtgtagaaa taaaagataa tatagtcatg atagatgatg
    12061 gcactggtac gttaaaacaa gccgttttca tagggtataa acaaatccaa attaatttaa
    12121 actgttcaca ttgtcaaaac ttaaatagaa taaaattaaa tttgtagaat tttaaaatgg
    12181 aaattattga aggaattaaa aatagaaagt gttaatattt agactgaaaa ttatatttta
    12241 tacaataaaa gtttatttaa ataacattgt attttaatat ttattttcag tcttacttta
    12301 ttaaaattat tttttggtta tgtctggaaa atatctgtct actaattttt ctgaaatatt
    12361 ttttaattgg tcagccattg aagtcagact taataaggct atcaataaat catctttatt
    12421 ttctggattt atactaatat tttcaaaatt agactctata aagtccggta tattctttct
    12481 ttgattttct tttattaaca tgaatttttc gtaacatgaa actaaatctt tttcactgaa
    12541 attatccttt tcatacacac catgtaagat tcttttaatt tcttgtattg taagtgtatt
    12601 tttcatagaa taaataatta tcatctgtaa aatttgttct ttagtatact tttttccctt
    12661 aactggtttg attaatccct ctttgctgta attatttatc atggttttag taagaatttt
    12721 atcactttca aatcttttat tagcagaaag cttattgtca attagagaaa taatttgatc
    12781 catatataag tcaatagatg gaatatcgtt tgaagttata tctgcattat ttagtgtttc
    12841 tagtattata gtattaagtt cattattcat catatgttac ctccataata tatataatac
    12901 actaaattag aaagtaaaac aatattaagg cttgtaaaaa aaacataaag tggtataatt
    12961 aaatatataa tatagtaatc aaaactatat ttatgaatct gatttttaaa atacataatt
    13021 gtaggaggac gaaatgtatc aatttttttt aaagggtaga gacccaataa gcagtttaac
    13081 tcattttata ggtgcttgtt tatctctttt agcaactata attttagttt ttcaatctgt
    13141 tacattacag gaaacttctc tcttgatgat agtatctgta tctgtatttg gactttcact
    13201 aattgcttta tatagtgcaa gttcgtatta tcatttttta aaggggacac cagaacaaga
    13261 attgtttttt agaaaagtag accatgcaat gatttacgta ttgattgctg gctcttatac
    13321 accaatatgt ctgaatttta tggaaaaaaa agaaggcata atatttgtta cagcaatatg
    13381 gatagttgca tttattggca taataataaa aatattttgg atggatgcac ctagatggtt
    13441 atctaccagt atatatttac ttatggggtg ggcaattgtt tttgatataa atgcattcaa
    13501 ttcaattcct aaggattgcc ttagactatt aattatggaa ggtgtatctt actctatagg
    13561 tgcgattatt tatataatta aaaagcctaa tataagtcct gagtttggat ttcatgaaat
    13621 atttcatatt tttattatga taggttcttt atttcacttt ttagcagttt tattgtatgt
    13681 tttatagttt taaaagattt gattaattga tactaaaatt ctacaatgaa taaaatggaa
    13741 ctgccttata tagattatat cttagacaat aagacagttc cttattataa atattaaatt
    13801 ctacttataa taaaattttg ataaaaatag tccattcaaa atgcctattc cataattcct
    13861 ttaatgtact tttcatttac gacatatagt ttgaatttat gtactataat tatatcctct
    13921 aaattctctg aaacactaac aattctgaca catttgtttc taatttctac aggtaaatct
    13981 aaatcatttg ttaaattcac atagtcatca tatttataca taaaatcatc cctttacatt
    14041 aagat
//
LOCUS       DAMKMI010000106        13948 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMKMI010000106 DAMKMI010000000
VERSION     DAMKMI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.110"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            154..3702
                     /locus_tag="PC429_002751"
     CDS             154..3702
                     /locus_tag="PC429_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3848243.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAVFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDEMVIDLMDSDVKRSSLIRM
                     TISTLDGECYSNDGITHNVQDRDYFKKAKNGEKNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVAKKNGDIVAQKTNKNRKTLSCIFQL
                     FDKDNCDEDDCLKTIKENMKNDKSGYFNYNLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYIFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSCEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVRKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLEDELNNKLIKLIFK
                     DDRTELINSINKQLKLSDTFASEQRVEKRDGTIIWILLKGRKMIYDNGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSQGSEYEECEIRLRTRDTIYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIANDLINLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRNYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKFDKVQYITNTRNAGDSDNVEMSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNRVCKNTFEWCNEGVTSEINNLKNICYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGNEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLSMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQDKPCKKCPMIYLKDGDDTYKSFMHNKKF
                     EVQANITTTTLKWFDGKDACLIFGSEITSIEAEENT"
     gene            3887..5821
                     /locus_tag="PC429_002752"
     CDS             3887..5821
                     /locus_tag="PC429_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF3848244.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5927..6922
                     /locus_tag="PC429_002753"
     CDS             5927..6922
                     /locus_tag="PC429_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3848245.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6894..7679
                     /locus_tag="PC429_002754"
     CDS             6894..7679
                     /locus_tag="PC429_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF3848246.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7667..8365
                     /locus_tag="PC429_002755"
     CDS             7667..8365
                     /locus_tag="PC429_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848247.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8488..8961
                     /gene="ybaK"
                     /locus_tag="PC429_002756"
     CDS             8488..8961
                     /gene="ybaK"
                     /locus_tag="PC429_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF3848248.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9158..9997
                     /locus_tag="PC429_002757"
     CDS             9158..9997
                     /locus_tag="PC429_002757"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF3848249.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            10010..10558
                     /locus_tag="PC429_002758"
     CDS             10010..10558
                     /locus_tag="PC429_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF3848250.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10624..11805
                     /locus_tag="PC429_002759"
     CDS             10624..11805
                     /locus_tag="PC429_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF3848251.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            12100..12486
                     /locus_tag="PC429_002760"
     CDS             12100..12486
                     /locus_tag="PC429_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848252.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12636..12899
                     /locus_tag="PC429_002761"
     CDS             12636..12899
                     /locus_tag="PC429_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3848253.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(13028..13870)
                     /locus_tag="PC429_002762"
     CDS             complement(13028..13870)
                     /locus_tag="PC429_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF3848254.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
BASE COUNT         5535 a         1478 c         2494 g         4441 t
ORIGIN      
        1 ttacaaataa atcttaagct atcttattta attctaatac aggaagaagg taaatgtata
       61 ttgaaaaaat catagaaata gaagtaaaca atttaaataa atacaaaggt ttgcaaaaga
      121 agtaatataa ttatatacaa tgaggatgtg agtatgatga acagactaaa gccaagaatt
      181 ttaggtatta gtatcttgtt tattattata attattttaa catgtggagc tgttttcaag
      241 tttaaattta aattagataa taaattaaaa gaaaccacac atacatattt gtctgagttt
      301 acagaacaca atataattaa tctcaagaca agacttagtg gtcaatttga catgcttgaa
      361 tctattgcta acttcatagg aaacttgaat aatataaatg atgaaatggt gattgattta
      421 atggattctg atgtaaagcg aagttcttta attagaatga ctatctctac attagatggg
      481 gaatgttatt ctaatgatgg aattacccat aatgttcaag atagagatta ttttaaaaaa
      541 gctaaaaatg gagaaaagaa tatatcagag cctttaaaat ctattgttaa taataaagaa
      601 ataatagttt tatctacccc agtttataaa agtgggaaaa tagttggtgt tttaagtgga
      661 atttatgatt caaaaaagtt aaatgagtta ttaggattat ctgcatttga tgaaaaagca
      721 gatgtttttg ttgcaaagaa aaatggagat atagttgctc aaaaaacaaa taaaaataga
      781 aagacgctat catgtatatt tcagcttttt gacaaagata attgtgatga agatgattgt
      841 ctaaaaacta taaaagaaaa tatgaaaaat gataagtctg gatattttaa ttataactta
      901 gataatgaaa aatttatgtt aaactatcag ccacttggag ttaatgattg gtatgttttt
      961 tctgttgtgc caaataaaat agtatctatt cagtcagaga atatatctta tgatgtatat
     1021 atttttgcat tagaaatact actagttata ttgattttta taatatatat agcatgtatt
     1081 ataaataata acaccaaaat tattataaat gaaaaagaaa aatttaaagt actcactgat
     1141 aatatattag gaggggttaa aacctgttta aaagatgaat atatgactat gacatatgta
     1201 agtcaggggt ttttagagct tacaggttac tcttgtgaag atttaaataa aatatttaaa
     1261 aatagattta aagacatgat ttataaagat gatttagaca agtttaattg tattattcta
     1321 aatcaattag aaaaacaaga taaaattgaa gttgaatata gaattgtaag aaaagatgga
     1381 agtataattt ggattttgga gagaagtaag tctataaatg ataataaagg aagtgagtgt
     1441 attcagggag ttttaactga tataacagaa cttaagaaaa tacaagaaga attagattca
     1501 aaaagatttg aaatacaggc tatcaatgat agtattgttg gtggaatagt gataactgag
     1561 ataaatgatg attttgatag tatatattta aatgaaggtt atttaaatat gattggatat
     1621 acacgcaaac aactggaaga tgagctaaat aataaattaa tcaaacttat ttttaaagat
     1681 gatagaacag aattaattaa tagtataaac aaacaactca aattaagtga tacatttgca
     1741 tctgaacaaa gagttgaaaa aagggatggc actattatat ggattttatt aaaaggaaga
     1801 aagatgattt atgataatgg aaaatctgta ctttgttgtg ttattgtaga tattacttct
     1861 gctaaattat tagagcaaga acttaaaaag agtgaagaac gtcatgaaat cattctaaag
     1921 cagacgcaaa atattataat agaatgggat atagaaaaag ataaaatgaa tatatcacct
     1981 aattggtata ataagtttaa atatgaccct ataactgaaa acgcaacaaa aaatataatt
     2041 aaatcagtag atatatatca tgatgatatg gataagttta tttcattaat taaacttact
     2101 tcacaaggta gtgagtatga agagtgtgaa attagactta gaactagaga tactatttac
     2161 gcttggttta gtataaaggc aacaacttta tttgataata atggtaaacc ttataaagta
     2221 gtaggaagtt tgactgatat tgatgaatca aaacgagaag ctgaaaagtt gaaagaaagg
     2281 gcagaaaaag accctcttac agatctgtat aataaaaaag catcacaatc acttatagag
     2341 gagtacataa cattggataa aaaaagaaaa ggtgctttaa tgattattga tattgatgac
     2401 tttaaaggaa taaatgataa tttaggacat ttatatgggg atgctgtatt aagtgaaatt
     2461 gcaaatgacc taataaatct atttagagca accgatatag taggcagaat tggtggagat
     2521 gagtttattg tatttataaa agatatttct gaaacaagag atattataaa taaagcagaa
     2581 gaattggtaa aaacatttga aaggtctttt ttaggtaaag gacgaaatta taaaatatca
     2641 ttaagtgtag gaattgctaa gtttccacaa gatggtatgg actatatgac actatttaga
     2701 aatgcggata tagctcttta ctcagccaaa gggaagggca agaattgtta tacattgtat
     2761 gataaaaaat ttgataaagt ccaatatata actaatacaa gaaatgcagg agatagtgat
     2821 aatgtagaga tgtcttttaa agaacatatc acaaattata tatttgatat actatatgaa
     2881 tcaagtgata tagaagtggc agtaaattta attctaagta ttgtaggtaa acactttaat
     2941 gtgagtcgca tgtatatatt tgaaaattca gatgataatc gagtatgtaa aaatacattt
     3001 gaatggtgta acgaaggtgt aacctcggaa attaataatc ttaaaaatat ttgttacaag
     3061 gatttaggca actatctttt aaattttaat gcagatgggt tattttattg taatgatatt
     3121 gaaaatttag gtagtgatgt gaaaaaagta cttaagccac aaggcatacg ttcaatatta
     3181 caatgtaaaa ttttagaggg taatgaattt agagggttca taggatttga tgaatgttca
     3241 tataatcgta tgtggactaa tgatgaagtg aacactttga catttatatc taaaacgctc
     3301 agtatgtttt tattaaaaat gcgtattcag agtaagctag aagagtcttt aacaattcaa
     3361 aaatctgtga tggataatat ggatatatgg gcatatgttg ttgacaggaa tacctatgaa
     3421 ttactattta tcaataaaaa aatgcttgag tcagcaccaa acactcatgt aggtgattta
     3481 tgttataaag cgatatggaa taatcaagat aaaccttgta aaaaatgccc tatgatatat
     3541 ttaaaagatg gtgatgatac ttataaatca ttcatgcata ataaaaagtt tgaggtacag
     3601 gcaaatataa ctacaactac tttgaaatgg tttgatggta aagatgcatg tttaatattt
     3661 ggaagtgaaa taactagtat tgaagctgaa gaaaatactt aaaatactgt gttatatata
     3721 tacttaaaat cgcaatttga atttattaga cttaaaatgt ctatataaat catttaagtt
     3781 gtggttttaa gtatttttta tgatataatt aagcgttaag aacaaaatta aaagatactt
     3841 ttgtataaaa aaatatttgt taaatatata ttgagcaggt gataatatgg aaaataagtt
     3901 tttaccaata agcaaacaag atatgataga tagaggatgg gaagaattag attttgttct
     3961 agttacagga gatgcttatg tggaccatca tagttttgga acagcaataa tatctagagt
     4021 tttagaaagt gctgggtata aagttggaat aatagctcaa ccagactgga gaactacaga
     4081 tgattttatg aagctaggaa aacctagact tgcatttcta gttaatgcag gaaatatgga
     4141 ttcaatggtc aatcattatt ctgtaagtaa aaagcataga gaaaaggata tgtattctcc
     4201 aggtgggaaa atgggttata gaccagatag agctactata gtatattgca ataaaataag
     4261 agaagcatac agtgatgttg caatagtaat aggtggaatt gaagcgagct taagaagatt
     4321 tgcacactat gattattgga gtgataaggt tagaaagtct atgttaatag atagtggagc
     4381 agatttatta gtttatggta tgagtgaaaa gcagattgta gaagtagcca atgcacttaa
     4441 tgatggatat gacccaaaat acattagaca tattgatggg acatgctata tttcagatac
     4501 attggaagaa atatatgata aatatatatt aattccatca tataaagaaa tttgtgaaga
     4561 taaaatgaag tatgtagaag cttttaaaat tcaatatgat gagcaagacc catttagagg
     4621 taatataatt gttcaacctc atggaagtaa gtatctggtt caaaataagc cagaaaaacc
     4681 acttagtaga gaagaactag atgaagtata tggattacca tatcagaaga cataccatcc
     4741 tgtgtatgaa aaatttggag gaataccagc tatagaagaa gtaaaattta gtatagtaag
     4801 ttcaagaggc tgttttggta gttgttcatt ttgtgcaata acatttcatc aaggtagagc
     4861 agtgcaaagt agaagtgaaa aatcaattat tgatgaagca ataggaataa caaatttaga
     4921 tgattttaaa gggtatatac atgatgttgg aggtccaaca gctaatttta gaagaccagc
     4981 atgtaaaaaa caaattacta aaggtgcttg taaaaataga caatgcttat ctccatcacc
     5041 atgtaaaaat ttagatgcag accacagtga atacttacat ttacttagag ctgtaagaaa
     5101 attacctaaa ataaaaaagg tgtttgtgcg ttctggaatt agatacgatt atgtaatggc
     5161 agataagaac aataaatttc ttagagagtt gatagagcat catgtaagtg gtcagttaaa
     5221 ggtagcccca gaacatatat cagaagaagt tttaaagtat atgcaaaaac cagcaggaaa
     5281 aacttatgat aaatttagac agaagttttt tgctataaat gaagaattag gtaaaaagca
     5341 atatttgata ccctatttaa tgtctagtca ccctggttct accttaaata gcgctataga
     5401 gttagcagag tatttaagag atactcacta ccaaccagag caggttcagg atttttatcc
     5461 aactccaggg actctatcta caaccatgtt ttatacaggt attgacccgc ttacaatgaa
     5521 gccagtttat gttcctaaat caaaacgtga caaggcgatg caaagggctt tattacaata
     5581 tagagcacct agaaattatg atttggtata ctctgcgtta gttgaagcag gaagagaaga
     5641 cttaattggt tttggacata gatgtcttat aaagccaaaa aatgaaaagc cttattttaa
     5701 tagaaataat tctaaaaaaa atgtatctaa aggaacaaat aaaaacaaaa aaactaatac
     5761 aaataacaga agtaataaaa atcaacaaaa aagttcaact aaaaaaaaga aaaaacatta
     5821 aagtataaat aaaaatactt aaaaaagaag gattattcga taaattaaca gaaattagta
     5881 taaataaata taattatttt aatgacgtta cctttgggag gaagagatga aaaagtatat
     5941 ttctataata ttattagtgg tgttgactat ggttgttttg gtaggatgtt caccaggaaa
     6001 agataaccct aaaaataagg agctatcagt tgtcaaacaa gtaaaagtag ctgttccaga
     6061 tggactacca gcagtggcaa tagctaaact tgcaaatgaa aatccagaga ttaaagaagg
     6121 atatgaaaca gtgtatagca ttgaaaagac acctgaagcg atttccacta gagtgatgaa
     6181 aaaagatgca gatatagcta tagttccttc aaacatggct gcaatagcgt ataataaaac
     6241 ttcatcatat aatattgttg gaactgtagg aatgggttca ctttatttgg tgtcaacaga
     6301 aaatataaaa gattattctg atttagttgg taaagaagtt ggatgtactg gaaaaggact
     6361 tactccagac ataactataa aaagtctttt gttacaaaag gatataaatt attcagatat
     6421 aaaatttaat tatgtaaatt cagcaagtga actggtacca ttattggcta ctggtaaagt
     6481 taaaacaggt attgttccag aacctgcact tagtgctttg atgtctaaaa atccagacat
     6541 aaaaattata aaaagtttaa atgactcatg gaaggaagta agtggttcaa aagatggtta
     6601 tcctcaatct actttaattg taaagtctga atttttaaga gataataaag attttgttga
     6661 ttcttttgta ggtcaattat caaatagtat agattgggca aacaaaaatc ctgaagaatt
     6721 aggtgcatat tctgaaaaaa taaaaatatc tactgaatca aagataatag gaaaatctct
     6781 tgaaagagct aatttaaaat atattccagt taaggacatg attaaagatt ataaaaatta
     6841 ctatgagaaa ttagctaatt ttgatgataa gacattagga ggcaaagtac cagatgaagc
     6901 aatatacttt gttgagaaat aaaaaaaata aagaacaatt tatagataaa atggaggtaa
     6961 cactttcttg tgttatcctc ttatttttat ggcaaattat tgctttaaaa attgataatg
     7021 atattttttt accaacagta ggtcaagtat ttaatagtat aaaagagatt atgctaaatt
     7081 ctagtttcta tatagatata ctttactcta tggggagatg tttttttagt tttttactgg
     7141 ctatgatttt tgctattata ctttcaatag tgtcttattt aaatagattt tttagaaact
     7201 tattaaagcc aatcaatgct ttgacacgtt caataccaac tatgatattg gtagtacttg
     7261 cacttatttg gtttgaaaag gatagtaccc cttttatagt tggatttact atagtttttc
     7321 ctatattgta tgataacgtt ttgggcgcta tattgaatat agacaagaac ttattagaaa
     7381 tggctaatgt gtataaaata aggtttatag ataaggtgtt aaaaatttat ttaccagcca
     7441 taaaatttca aattatatct atactagtat caacattctc tttaagtttg aaagttgcta
     7501 tagcaggaga ggtatatagt caacctacat atggtattgg aactatgata cagactgaaa
     7561 aaattaactt taacacatct ggaatatttg catggataat tattgttctt ttaatatctg
     7621 ctattttaca aatagctcaa aagtttttag caaggagagc ttttttatgg aaaagataaa
     7681 gttgagtatt gaaaatataa ataaaagata tgatagtcgt attatattta gagattttaa
     7741 tattgatttt tatgtaaatg aggtaaattg tatccttgga aaatctggtt gtggaaaaac
     7801 tacactcttg aacataataa gtgggattat taaaaatgac accaacaatc taaatatcaa
     7861 agaaaattta aatagagtag gaaataagct tgaagctagt tacatatttc aagatgatag
     7921 acttatagac tggttaacag tagaagaaaa tataaagata gttgtaaata aatactataa
     7981 taaaactcaa ttaaataaaa tttgtgatga atacttggaa ttggttggaa ttagtgatta
     8041 taaaaaattc tatcctcaga tgcttagtgg aggtattaga caaagagtaa atattgctag
     8101 agcttttata tatccatcaa aaaatattat aatggatgaa ccttttaaat ctattgatgc
     8161 aaagaataca cagcttataa tggataattt cagaaatata ttaagaaaag aaaaaagaac
     8221 tgtgttattt gtaactcata atattgagga agcattgttt ttagcagata ggatatttat
     8281 tttaggagac tctccaatta gaataaaaaa aatattaaaa aattctaagg aattggaaaa
     8341 aaatgaagtt ttgaagttga tatagagtta taataatact gtcgttttaa aatatatttg
     8401 gttaaaatct ataagactat attaaaatag tacagaatat tcaaaactgt gtataatgtt
     8461 cgtattttat aaaaaggaag tgaatgtatg gttaagacta atgctatgag aatattagat
     8521 tcaaataaga ttgattataa agtaatgtct tatgaagtga aaagtgaaca tgtggatggt
     8581 gttgaggtgg cacatgatat aggaagagat gtaaatgaag tgtataagac attagtaact
     8641 caaggagtta gtaagaatat atatgtatat gtaataccag tacatgaaaa cttagactta
     8701 aaaaaggctg caaaagtagc taaagagaaa agtgtagaga tgattcatgt aaaagatata
     8761 aataagctta caggttatat tagaggcgga tgttctccta taggtatgaa aaagctttat
     8821 aaaacttttg taaatgaaag tgcaaagaat ttagatacaa ttattgtgag tgcaggaaaa
     8881 ataggatatc aaatagaact atctccattt gatttacaaa gacttataaa agtagaattt
     8941 gtagatgtaa taaaaaaata agaattacat ataaaatata gagcttatat tcttaataat
     9001 aggtgttgta gtattaataa aatgaataat aattttaaaa taaatattca gttacttatg
     9061 tgtaattggt ataaaaatat tgagaaattt taaaaacaat ggtaaagtgt aagtataatc
     9121 acgaatattt tttataaagt aatggaggga tgttcgcttg agaaaaataa aatctgaaca
     9181 aatcgtcgag caggtaaaaa aactttgtat agaagcgagc ctatatttag gagaagatgt
     9241 tttaagctgt ataaaagaaa aagcgaaaag tgaaaagagt gaagttggaa aaaatatatt
     9301 gaatatatta gttgaaaatg ctgaaatagc taaagaaaaa aatataccaa tatgccaaga
     9361 tactggtatg gctgtatttt ttgttgaaat aggacaagaa gttttaattg aaggagacac
     9421 acttactgat gctataaatg agggagtaag acaagggtat gaagaaggct acttaagaaa
     9481 atctgttgta agtccaatta acagagtaaa tacaaaagat aatacacctg ctgttattca
     9541 ttatgatatg gtaaaaggtg ataaaattaa aatagaattt gcagctaagg gatttggaag
     9601 cgaaaatatg agtaagatga aaatgttaaa gccatcagat ggattggaag gaattaaaaa
     9661 attcataata gatacagttt cagaagctgg acctaaccca tgccctccta tggtaattgg
     9721 agttggtata ggtgggactg tggacaaatg tgctcaaata gctaaaaagg cactttttag
     9781 agaactaggt gaatttaata aggacgaaaa tatagcaaaa cttgaaagtg aactattaac
     9841 agctattaat aaacttggta taggacctca aggcttaggt ggtactacaa cagctttggg
     9901 attaaacata gagacttttc ctacacacat agcaggttta cctgttgttg taaatataaa
     9961 ctgccatgca tcaagacata agaaggtagt aatttaggag gaaaataaaa tgataaaaat
    10021 aactacacct gtaaatgaaa ttgatatagc aaaattaaat tgtggagata caattagttt
    10081 gagtggtata ctttatacag caagagatgc tgctcataaa agattgatag attgtataaa
    10141 taaaggtgaa gaattaccat ttgacgtata tggtcaagga atatattatg taggacctac
    10201 tcctacaaag ccaggagaag ttataggtgc tgcaggtcca acgacgagtt atagaatgga
    10261 tgatttgact atacctcttt tagaaagagg tcttagattg atgataggca agggaaaaag
    10321 aagtgatgaa gttatagaag gtatgcaaaa atacggagca gtttatcttg ctgcaatagg
    10381 aggagctgga gcatatattt caaattcaat aaaatcatgt gaaattattg catatgaaga
    10441 tttaggagct gaagctataa gaaagattgt agtagaagac cttaagctta cagtagcaat
    10501 agatagctat ggaaataata tatatgagca aggtcgagca atttatgaat gtaaataatg
    10561 tttagaaata aatatgaata tcaaaattaa aaatagttgt gtatatttta aggaggaaaa
    10621 ttgatgtcta aggattatgc aaaattggca ttagaaatgc atgaaatcaa caaagggaaa
    10681 gtaagtgtag aaagcaaggt agaagttaaa actaaggatg atttatcaac agcatataca
    10741 ccaggggttg ctgaaccttg tttaaaaata catgaaaatc aagatgatgt ttatagatac
    10801 acttctaaag ggaatctagt agcagtagtt agtgatggaa gtgctgtact tggattaggt
    10861 aacattggtg ctgaagcatc tatacctgtt atggaaggta aagctatatt atttaaacaa
    10921 tttgcagatg tagacgcttt tccaatatgt ttaaagacta atgatgtaga tgagatagtt
    10981 aaatctgtag agttgatgga gcctgtattt ggaggtataa atttggaaga catatcttct
    11041 ccaagatgtt ttgagataga agagagatta aaaaaatctt taagtatacc tgtttttcat
    11101 gatgaccaac atgggacagc aataatagtt gcggcagcca taataaactc aataaagtta
    11161 attgaaaata aaaaaataga agatttagaa atagttataa acggagcagg agctgctggt
    11221 atagccatag caaagatact acttaatatg aatgttaaaa atataatctt atgtgataga
    11281 agtggagcat tggaagctag tatagaaaat ctaaactatg ttcaaaaaga aatgttaaaa
    11341 gtaacaaata taagaaatga aaaaggacct ttaaaagata ttataaaagg aaaagatgta
    11401 tttattggtg tttcaggtcc aggagctgtt acaaaggaaa tggttgaaag tatgtctgag
    11461 aagccaataa tattggcaat ggcaaatcct actccagaga taatgccaga agaagcaaaa
    11521 ttaggtggag catttataat gggaacaggt cgttcagact tccctaatca agtcaataat
    11581 gtactggctt ttcctggaat atttagaggt gcacttgatg ttaaggctaa agagataaat
    11641 gaagaaatga agatagctgc tgcatatgca attgctaata caataagtga tgaagaaata
    11701 tgcccagagt atatattacc agatgtattt aataagaatg tagtaaaaaa tgttgctaag
    11761 gcagttaaag aagcagcgat aaaaaccaaa gtaaatagaa tataaatttt tatatgttca
    11821 tatatcctta tattatcggg tatatgggca tttttattat aggtttatac tcatatatcc
    11881 ttacttttat atacatatct ttatgattaa aggtgttaga aaatgtagta aattttatac
    11941 tatcagactc tatctttatt actttagtag atacatggtt gtaaaggttt atagaaaaaa
    12001 atataggaag gcagattttt attgttgcgt atatatgaga atgatataat atgaatatta
    12061 taaattaata aatttaaatt tatggaggta caagagatga tggaatataa agttaatgac
    12121 caagaactta atgcttcaat gtttatccca ttcgtcaaca ggatatggcc aggagattat
    12181 gatatggata aaacacagac tgcattatca aaaactttga atataactgc ttatgatgat
    12241 aaagtgcttg taggatgtct gcgagttctt tcagatggtt attattttgg aacaattaca
    12301 gaattgcttg ttcttccaga gtatcagaaa caaggcattg gtagtaagct tttccagctt
    12361 gctaaagaga acaccccaac tatgctatat tttggtgcac agccaggagt agaagagttc
    12421 tatgaaaaaa atgggtgcca aagaagtctg caatcctaca taataaaaaa gaaagatagt
    12481 tattaaagtt ccagctttca ataatcttaa aagtacaata ttttagtgga agcatttcat
    12541 tttttattac taatatgaca taaacatgtc atattagcat tgatatactt ttaatatggt
    12601 taagagccaa agaaaaatat aaggagtgat ttaaaatgga agaaactaaa ttttgccaat
    12661 gctgtgcaat gccaatggga caaactgatg aattatatgg gacaaataaa gatggaagta
    12721 aaagtactga ttattgtagt tattgtttta aagatggtga gtttacagct gatataagta
    12781 tggaagagat gatagaagtt tgtatccctc atatgcttca aagtaataag gatatgactg
    12841 aagatgaagc aagaacaatg atgaacaatt tctttccaac attaaaacgc tggaaataat
    12901 tttatgcaaa attagttata tgcaaaacat gaaatatggt gttgagaata aggggtggtg
    12961 tgctttatag cataccacct gttttgttac ttggagttca gttattttcc taaatatttt
    13021 aaatatacta ttttagataa gttccctctt catcttgata aattaatcct tttttcataa
    13081 gcactccaag agctcgttta aaatttttct tacttgaatt aaataatata gaaatatctt
    13141 taggatcaga tttatcatta aatctcatat atccatcaga accttctaaa taacttaaaa
    13201 tcttattttc taaagtatca agttcatcct ttctatttcc tctaggagaa agaccaagtt
    13261 ttccatcttc atatattttt atgacatgta aattttcaag aacatcacca gtttttagtt
    13321 cagtaaagta ctcattatga agtattacac cagcatattt attgtctaca catatcattg
    13381 cagaattatt agtttggaat ccatatacaa ctccagttac aatatctcct acattgtaag
    13441 tatggtctgt taataaatat gaatctatat ctgtagtagc agcaattctt ttgcttttgt
    13501 ctaaatatat ataaaataaa tatttttcgt ctctaaatag tggataagtc ttagctttaa
    13561 aaggaactaa tatgtcttta ggaagtccca tatctataaa agtaccaatg tctgtattat
    13621 caactacttt taaatgtgct acatccccaa ccttagctaa aggcggtatt aaagttgcag
    13681 cagttctgtc atcagaatct ttaaatatga aagcatttac ttcatcacct atgtttattt
    13741 cattttttcc gattaatcta ttatgaagaa gtatatcgtc ttttgtgtta ttagtttgtc
    13801 catccaaaaa atatccaaat tcagtttttc tttttactgt aagtttatta aaatcaccta
    13861 tgtttatcaa attaaaatca tcctctcatt ttatatttat aatttagcca taatatactt
    13921 atgtagtagt ttgtcacagt atctatat
//
LOCUS       DAMKMI010000107        13884 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMKMI010000107 DAMKMI010000000
VERSION     DAMKMI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.111"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            154..516
                     /locus_tag="PC429_002763"
     CDS             154..516
                     /locus_tag="PC429_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF3848255.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            952..1653
                     /locus_tag="PC429_002764"
     CDS             952..1653
                     /locus_tag="PC429_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF3848256.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1923..2741
                     /gene="larE"
                     /locus_tag="PC429_002765"
     CDS             1923..2741
                     /gene="larE"
                     /locus_tag="PC429_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF3848257.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2796..3944
                     /locus_tag="PC429_002766"
     CDS             2796..3944
                     /locus_tag="PC429_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF3848258.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4080..5468
                     /locus_tag="PC429_002767"
     CDS             4080..5468
                     /locus_tag="PC429_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF3848259.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5497..6195
                     /locus_tag="PC429_002768"
     CDS             5497..6195
                     /locus_tag="PC429_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848260.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6198..7631
                     /locus_tag="PC429_002769"
     CDS             6198..7631
                     /locus_tag="PC429_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3848261.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7653..8390
                     /gene="truA"
                     /locus_tag="PC429_002770"
     CDS             7653..8390
                     /gene="truA"
                     /locus_tag="PC429_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF3848262.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8501..8708
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8961..10160
                     /locus_tag="PC429_002771"
     CDS             8961..10160
                     /locus_tag="PC429_002771"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF3848263.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10407..11453
                     /locus_tag="PC429_002772"
     CDS             10407..11453
                     /locus_tag="PC429_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF3848264.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            11561..12724
                     /locus_tag="PC429_002773"
     CDS             11561..12724
                     /locus_tag="PC429_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3848265.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12813..13193)
                     /locus_tag="PC429_002774"
     CDS             complement(12813..13193)
                     /locus_tag="PC429_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF3848266.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13196..13855)
                     /locus_tag="PC429_002775"
     CDS             complement(13196..13855)
                     /locus_tag="PC429_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF3848267.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
BASE COUNT         5601 a         1355 c         2380 g         4548 t
ORIGIN      
        1 aggactattt aaatatataa tgtgaataat gtttaataat ttgaaatatg ataatttttt
       61 atgttgaaag cttataatga ttatgatata aattggttat agtatacaat ttatgggtat
      121 aacaataaaa aattagaaag gaatttttat aaaatgatta aattatatgg atatacaaaa
      181 tgctctactg ttaaaaaagc taaaaattgg cttaaggaaa ataatttaga atttgaagat
      241 atagatatgg ttcaaaaccc tcctagtaaa gaggagttaa aatcaattta taagactagt
      301 ggatatgata taaagaaatt ttttaataca agtggtatga aatatagaga attgggtctt
      361 aaagatattg taaaaactga atctgatgat aaacttttag aaatattggt aagtgatggt
      421 atgcttatta aaagaccatt acttttggat ggtaaaaatg tattattggg ttttaaagaa
      481 gatgtatgga aaagtacttt gctaaaagaa gattaggaag agataggtgt gttgattaaa
      541 attattaatt aatacaccta taaatttatg tattaaatct aatctttata aatattaatt
      601 agcaaattca aatcaaaaaa attcatatca acaaacatat tacttcttat tacaaattat
      661 atcatatgtt ttatattaaa atattataat attacaacga attgttacaa aattacttat
      721 tattaaccat gaacatttgt tttgtgctat catatttata taagggaatt gtcataaaaa
      781 atatcttgtt catacagttt tatatagaaa aaagaaatga atcttttctt tatataagta
      841 tattttgtaa attgcctcta tatttatagt ttaaataata gtggtattaa aaatctgaca
      901 acaaaaataa aactttcaaa aaattaaatc taaaaagaat aggagataaa tatgagtatt
      961 gaagatttat atgattttga atgtgttcct ttgaacaatg agattgatat tgatgtttta
     1021 tttgaaaaga tgatagaaga tggaaaagaa aaagaatata caccaattat agtcattgat
     1081 gaaaaagtag ggctattaag aaaaaatata gatatgatta ctaaagagta tggttctctt
     1141 gaaaactata gagagtattg tttaacaaaa tataatgaaa ttgatgtaaa tcaatttttt
     1201 gattttagga agagtgagat ttcagaatta ttaaaggagc taattgattt agaagatgat
     1261 ttttatgatt ataaacattt tagacccatt atgaatatag atatatttca tgaatataac
     1321 aaagtttaca tagctaaaat tccaacaatt aaaccatatg aagtatttgc atacatacct
     1381 attggcggat ttaatgattg tcctagagat gaagaacata tagctattgc aaaatattgg
     1441 tatgaaaagc atggaatctt tccaatcgca attggatgtg atacagttca atattctgta
     1501 aaaaatttaa ctaaagatgg taaaaaattt gatgacttat gtattgaatt agtattttat
     1561 tgtgaggata ttattataca agggtatgaa accttaaagg ctcttaagga tgtattaaaa
     1621 cgctctacaa tagtgctttt ttggtgggat tgatattcta tcatttagat agtattaaat
     1681 tgttagtcac caagaatcca cttgctttag ttatagggag tgtgtcaaac taggttttta
     1741 tacacagtgt agcatagtaa tatgtttaaa ttacaaaggt taaaaaaata ctttaatatt
     1801 tttaatatta taattaatag atttaaggta attataattt ataaaataaa tactttatat
     1861 gttataatca ttaagaatgt aaaaaaataa tatatagttt tatagttcat atttttccag
     1921 atatggaggt aaattttgtg aaggaaaaag aaaaattaga taaattaaaa aaaatgttac
     1981 ttgaattagg cagtgttgta gttgcttatt ctggtggagt agatagtaat tttttgctta
     2041 aggtcgcaaa agatacatta ggtgaaaatg ttatagctgt gactattcat gcaatgatgc
     2101 attcaagtag ggaaattgaa gaggctaaac aatatactca aaattttggt gttaaacata
     2161 tcatattaaa tatagaaaac tttgatttaa aagaatttaa agaaaatggt gttgatagat
     2221 gttatcattg taaaaaatat atattctcta aaataaaaga ggtagcaaaa gaacataata
     2281 taaagtacat agtagatgga acaaatatag atgatttagg agactataga cctgggctta
     2341 aggcattgag tgaattagga gtaattagtc cattaaaaga tagtagttta aaaaaagaag
     2401 aaattaggtc gctttcaaaa acattaggat taaatacttt taataaacca tcatttgcat
     2461 gtcttgcgag taggatacct tatggagttg aaataacaga tgaaaattta agaatagtag
     2521 aaaagagtga agaatatcta tccaatttag gtttttctca atttagagtg agaatgcatg
     2581 gagatatagc tagaatagaa gttggacagg aagagctagg taaatttttt gaaaataaca
     2641 attttaataa agttgatact aaattaaaaa tatttggctt taaatatgtt acactagata
     2701 tgtcaggata taaaatggga agcatgaatt taaatgtgta gattatttta aaaataaata
     2761 agttttatag ttaatataag gaaacaggag gttttatgaa aaattatacg ttaatatcgc
     2821 catgtttttt tggaatggaa aaaatgctgg ctagagagat tactaattta ggatatgaaa
     2881 taataaaaac agaagatgga agaataacat ataaaactga tgagtttgga atagcaaagt
     2941 caaacatgtg gttgagatgt gctgaaagag tacatcttaa aattgctgaa tttgaggcaa
     3001 aaagctttga tgaattattt gaaaatacaa aaagaataaa ctggtctaga tatatacctt
     3061 atggagctca atttcctata tcaaaagctt cctctataaa atcaaaatta tacagtacac
     3121 cagatgtaca agccatagta aaaaaggcta tagtagaaag cttaaagaaa agttatttgg
     3181 aggatggttt gcttaaagaa gataaggaga aataccctat ttttgtattt atacacaaag
     3241 ataaagtaac catttctata gatacaacag gagatgcttt gcataagaga ggatatagag
     3301 aaaaggcaaa taaagctcca ataagagaga ctttagctgc tgggcttatt tatttaactc
     3361 cctggaaagc aggtagagtt ttagtagacc ctatgtgtgg ttctggtact atattgattg
     3421 aagctgctat gattggtata aatatggcac ctggcttaaa tagagagttt atatcagaaa
     3481 agtggagaac tcttgataag aaaatatggt gggatgtaag aaaagatgct tttaataaga
     3541 tagataatga atctaaattt aagatttatg gatatgatat agatgaagaa tctatagata
     3601 tagctagaga aaatgctgaa atagcaggtg tagatgaata tatagagttt aatgtaggag
     3661 atgcaactca atttaaaagt gaagatgagt ttggatttat aatcactaat cctccttacg
     3721 gagaaagatt agaagataaa gacagtgtta aacagttgta taaggagctt ggttatgcat
     3781 ttagaaaatt aaaaaattgg tcgtattatt tgataacttc ttatgaagac tttgaatatg
     3841 agtttgggca aaaagcagat aaaaaaagaa aattgtataa tggtatgtta aaaactaact
     3901 ttttccaata tccgggtcct aagcctccta gaaataataa ataagtttta catgatttat
     3961 ttgtaaaata taaaatttta ttaaaataag taaaacatta tgatgccctg aaacgtctaa
     4021 ttattgtgag gagatttttg tgaacaaatt taaagattta taggattagg aggagttaaa
     4081 tgaaagtgtt aaagcttatt actgtagtag ccctgatgat gttatttgtt actggatgta
     4141 atataaaaac tgaatctcca gaacaattag cgaagatacc tgactatgat gatacaaaaa
     4201 aagtattgta tgatggtatt gaccagttac taaaaccaga ttcatctatg atattgcctt
     4261 ctaatacaaa agaagtagga aaaataaata aggtagattt aaacagcgat ggaattgatg
     4321 aattagtagt atttgaacaa aaagaagatt taagtaataa tgtgagtcag gtagggtttg
     4381 taaccttgag ttacaatgga gaaaaatacg tacttggaga ccatttttta gaaaatggcg
     4441 aatcaataga atatgctaat ttttatgatt tggactcaga tggatataag gaagttatct
     4501 tattggttaa atccaaagac aaaacgaata tgcatatata taaagtagta gataatgaaa
     4561 taacaaaaat atatgacttg gatgcatcat ggcttcaaaa taaagaagat tttaatgaca
     4621 tgaaagtaaa aattggatat atagatggag atgacaagtt agacatattg atgcttcatt
     4681 tgaataataa gactaatgaa atgtttgcaa gtgttgcaaa ttttgatggg aaaatgaaaa
     4741 tcaaagattc tgttaagttt gaaaatgtaa aaaacttaag tgaattatat ataacattag
     4801 gtaatgttgc ttcatctgtt ggaaattctt ctgcagctat aaaaggtatt gtacttgata
     4861 ttcctatatt gaaagataat aactatatta cgcagatact ttatatgaaa gatgggaaaa
     4921 ttaacaaagc ttttagtgat tatgataaaa ctattactaa atcttattat atacctgtag
     4981 atgatattga caaagataag attatagaaa tacctatagt tgcaggcagt acaggtaata
     5041 ataaaaatac ttatagttct aaatcatctg caactataag ttggtataga tggaatggaa
     5101 aagaaggtgc tgactcaagt ttaatattta caagtcagat atattataat tataaatata
     5161 attttaaatt atttattcca aataatttat ttgataaaat acttgttgaa caggaatttg
     5221 ttggagaaaa agcagtattt aaattttact attttgacta ttcagaccgt aagaatttat
     5281 ttaatatagt agtagagagt aaaaataaac ttgaagatag aaaaaataca ggtactcaaa
     5341 attctattgt acttcaagag agcgatgaat atactttctt actagttgta aacaatgcaa
     5401 atgaaatgga taagctagat atgactatag acgcattaaa agaatatttc tcattaatat
     5461 atggttaata ttatactcaa ttggaggttt tattttatga aggaaaagat acttatacta
     5521 gaagatgaaa ttggtattag aagttttgtt agtataaact taaaaaggga aggctatgaa
     5581 atagttgagg caggtactgg aagagaagct atagaaaaaa tgacaacaga aaaggatatt
     5641 acaatagctc ttttagatgt aatgcttcca gatataagtg gtatagaagt atgtaaattt
     5701 ataagggaga attttgacca agtaggtata ataatgctta ctgctaaagc tcaagaagat
     5761 gataaaatag aaggatttat ctcaggagca gatgattaca tcataaaacc atttagtata
     5821 aaagagcttt tagtcagagt ttctgctctt cttagaagag tagcaaagga tgattcaagt
     5881 gttaagtcga gtgagatagt ttctccacca tttatacttg atatagacaa aagaaaatta
     5941 tttaaaaatg gaaaggaaat tgagcttaca ccaactgaat tttcaatagt gaaatatcta
     6001 atatcaaatg ctaagcagtc gttaagtaga gaccagatat tagacgaagt atggggaact
     6061 aactatttat atgattttaa aatagtagat gtaaatataa gaaggataag aaataaaata
     6121 gaagatgacc catcaaaacc aaaatatata caaactattt ggggttatgg atactgtttt
     6181 agaaaggaag aataatagtg ttacattttg aaggtctgag aaaaaaagta ataaaaaact
     6241 attttataat aattattata atggttactc tgtttgaagg attgttcatg ttttacatac
     6301 aaaactacta ttatgattct gtcaaacagc tacttgaatc tgaaataaaa tatgctgatg
     6361 aatataatgc tattacaatg gaaactacaa gttttgagaa aaaagtaaaa aatatatttg
     6421 ataaacaacc cttaactaaa aattcagaat ttggaatttc aataatagat aaagataaaa
     6481 atattatatt agaccaatat ggttttaaaa gtaaagaaaa agcaaactat gaagatgtta
     6541 acaatgcatt aaaagatata aaaactaaaa atttaacacc ttatacttat agaataccag
     6601 atacagggga gcatgttatg agtatctcct taccacttaa ggtaaataat ataatagaag
     6661 gtgttgttag atatacagta tcattagatg ctattgataa tgcaatatta aagcaagcta
     6721 catggcttat attagcagga atatttatac ttattatagc tatactaata agtcttaaat
     6781 ttgctgagac tcttataaaa ccattacgag agctaaagaa atttgcaaat gaattggctg
     6841 taggaaatta taatataaaa ttagaaaaga tgaagattgt agatgatgaa attggtgatt
     6901 tagcacagac atttgaacac atggcacatg agattgacaa aagtgaaaaa ttaaaagagg
     6961 aatttatatc atctgtatct catgaactaa ggactcctct tacatctata aaggggtgga
     7021 gtgaaacctt aggttatgaa agtataacaa gagaagaact agacttaggt ttaggcatta
     7081 tacaagatga aacagaaaga cttataaaac tagttgaaga attattagac ttttcaaggc
     7141 tttcttcaga caggataaaa cttcatgttg atatagttga tgttgaaggt ttaattgtag
     7201 gtgttgtaaa tcaacttaaa gttaaagcag ctgaaaaaga tatatcactt ttatttgaat
     7261 ttgaaaatga atttatagag aacattcaag gagataaaaa tagattaaga caggttctca
     7321 taaatttaat acaaaattca tttaaattta cgagtcaagg tggttacata aaagttgtag
     7381 cttctcaaga tgaagagata acaaccatat cagtagagga taatggttca ggtatagaaa
     7441 agcaaaattt aaataaagtg ttagataaat ttttccaaga agattataat aaggctggta
     7501 gtggattagg actagcaatt agtaatgaaa ttgtaaaact tcatggtgga agaatgaaaa
     7561 tagaaagtga aaagaatgtt ggaacaaaaa taacttttaa tataaaaaat aaatttgcta
     7621 aacaagcata aattcttaga aagaggatta agatgagaaa cataaaaatg atagtagcat
     7681 acgatggttc aagatataaa ggatatcaaa aattaggtga caataatatg actattcaag
     7741 aaaaacttga aaatgtttta agtaaaatga ctaatgaaac agtggagata ataggttctg
     7801 gaagaactga tatgggagct catgcaagag gacaagtggt aaattttaga actaattgta
     7861 tggattcttt ggataaaatt caaaaatatt tatatgaata tttaccagaa gatatagttg
     7921 taaaaactgt tgaagaagtt gatgaaagat ttcattcaag atacaatgta aaatcaaaga
     7981 cttatatgta taaaattgac aataataagt atcataatcc ttttataaga aaatatgcta
     8041 ctcatgtaag taaaaaatta gatttagata gaatgagaaa agcaagtgag tatttagtag
     8101 gagaacatga ttttactagt tttgcttctt ctaaatctaa gaaaaaatca aatgtaagag
     8161 aaatttattc tataaatata aaagaagatg ataatgttat agaaatatat gtagaaggaa
     8221 atggattttt gtataacatg gtaaggataa tagtaggtgc tttgatagat gttggtctaa
     8281 aaagaagagc tccacaagat attaagtata tgcttgaatc aaaagataga tgtcaatcat
     8341 cagatactgc accagctaaa ggactatgtc tttggaaagt aacatattaa tcaattatat
     8401 aaaactctct ttaaaataaa aataaaataa attatatagt tgacataaat ggaaaaataa
     8461 actattatat attttataag agagatataa aactttatat ttaaatcctg tgagaaagag
     8521 agtaacttct atgtgtttta tagagagctg aggatggtgg aagcttggca aataagtatt
     8581 agtgaaaaga actttggagg atactaattg aatttagtat gttagtaggt aatctcgcct
     8641 taagagaaag agtggataag tttatcttat caattagagt ggtaacgcgg atatatttcg
     8701 tctcttggtt tttccaagag gcgtttttta atatatttaa ttattagtgt ttatctggta
     8761 tgttgaacat ttactaattg attagctaag tttatcaata agagtggtac cgcggatatt
     8821 tcgcctctta gtattttaaa atactaaggg gtttttttag tattaaaatg attttatgtg
     8881 agtctaaatt ctcagagtca ttcaatatta agaatttcta taaaataaaa attataattt
     8941 aagattggga ggaattaaaa atgaaggaaa aagtagtatt agcatattca ggaggacttg
     9001 acacatcaat cataatacct tggttaaagg aaaattatga agatatagat gttatagctg
     9061 tttgtggaaa tgtaggtcag gaagataaaa tggaagatgt atatgaaaaa gctttacaaa
     9121 gtggagcatc aaaggcatat gtggatgata taagtgaaga atttgtaact gaaactatat
     9181 ttaaagctgt aaaagcagag gctaaatatg aaggaaaata cttattagga acttctttag
     9241 caagacctat aatagctaaa aaattagttg aagttgctca taaagaaggt gcaaaatata
     9301 tatgccatgg atgtacagga aaaggtaatg accaagttag atttgaagct acaattgcag
     9361 ctcttgaccc aactataaaa gttattgcac cttggagaat ttgggatata aaatcaagag
     9421 aagatgctat agactatgct gaaaaacata atataaaggt aacagcaact aaagctaaaa
     9481 tatattcagt agatgccaac ctttggcatg tttcaacaga aggtggagat atagagcacc
     9541 tagaaaatga acataaaaaa gatgtttata aacaatgtgt agacccagaa gatgcatgtg
     9601 atgtagctga atatgtagaa gtttactttg aaaaaggtgt acctaaaaag gtaaatggcg
     9661 aagaattatc tccagttgca ctgattcata agttaaatga gttgggttgt aaacatggaa
     9721 ttggtgttat agatatagtt gagaatagac ttgttggtat gaaatcaaga ggtatatatg
     9781 agactccagg aggaacaatc ctttatgaag cacataatat tttagaaagt gctactttag
     9841 acaaagatac attacatttc aaacaaatgg tatcttataa atacggagaa cttatttaca
     9901 atggactatg gtattgtaaa ttaagagaat ctatagatgc atttatggaa caaactcaag
     9961 ataatgttac aggaacagta aaagttaaac tttacaaagg aaatataaaa cctgctggaa
    10021 tatttactga aaatgctctt tatgatgaag gaatatcttc ttttggaaat agtgaacttt
    10081 atgaccacaa agatgcagaa ggatttatta atttattcac tttaccatta aaaatcagag
    10141 ctatgaaagc tggaaaataa taaaataaat tatttaagag tgtataaaaa cgatttttag
    10201 gtttttatat gctctttttt tatttgaatt tattaaatta tacagtaaat agtataaaaa
    10261 aatatattat agttccaaat ggcttaataa tgtgaaaata taagacctaa aatataaatt
    10321 ttataaatac aatagttatt gactctctca ttgtaagagg gtttatactg aatctaatga
    10381 aaatttatag tgaggagata gattatatga gaaaaataat tgaaattaaa catcattcac
    10441 atgattatga gtgtatgtgg aatggtatag aagatttata tataaataaa accggtgaat
    10501 accttccaaa cgattttttc tttttactgt caggttttgg ttcattttgt tatatgaaaa
    10561 caaataaagc tgatttgaaa agaatggtag ctttgggaga tggaaggaca aaacagatgt
    10621 ataaattttt agcaccaatt gttggatttg aatacaaaca tcatgagttt aaaaagtatg
    10681 agcaagcatt aaagaaagca aaatctgaaa ttgatgatgg atatccagtt attttaggag
    10741 ctttagatat gtattatcta ccatattata agaagctata tcataaagaa catattcctt
    10801 ttcattatat attaatggta ggatatgatg atgaaggaat atatctgtat gattgtggta
    10861 gaacagaatt attacatctt tcttatgata aattacgtga aagcatgaat tgcaattccc
    10921 ctggattaag caaagaaaat actatttgca caataagaat gaatacttca aagaataaat
    10981 ataacattgc tagagaagct attgctataa agcgtgatat gtttttaaat ccacctaaag
    11041 gatttttggg atataaaggt tttgaaaagt ttattaaaga gttgccacag tggaacactg
    11101 aattggataa ggatgattat gataagattt tgaccaatat ggttatgttt tttggaacag
    11161 taccaacaat tccaaatgca atcaaaggaa ttaaagaacc agatactgtt caatttaaag
    11221 gtggatttga taaaatgagt agaatgctca acagtattgg ggaggaatac aaaaacaact
    11281 cttttataca agtagctgat atttttgata aaactgctat tataattgag aaaatatcca
    11341 atattataat agattactta actcaaacat gtgatgatac agaacaactt ccgttattgt
    11401 tctctgaggt cttagagcat atgaaaacag gatatttaat cttggatgta taaaaaatat
    11461 attgttgacc ctaacattac gtaatagtgt aatatgaaaa tatagcttaa atatattagt
    11521 aggtattgat ggctgtaaaa ttaaaatttg aggtgtggat atgagaacag tgaaacaagt
    11581 ttctgattta actggaataa gtgtgagggc attacattat tatgatgaaa taggtctttt
    11641 aaaaccaaat aaaattacag atgcaggata tagactttat gatgatgaat ctattaaaac
    11701 tcttcaacaa attttatttt ttaaggagat tgatattcct ttaagagagg ttaaggaaat
    11761 aatgtcaagc caatattttg ataaggttga agctctaaaa aatcaaaaaa aattacttat
    11821 tttaaaacga aagagactgg atgaattgat agagcttata aatcaaactc taagaggaga
    11881 aggaaatatt gattttaaag agtttgatat gagcgagtat tttaatgttt tagaagaatt
    11941 taaaagagag cataggaata aggtaattaa aatttatggt agtgtagaaa aatacaatga
    12001 atatatagaa agagttaagt ctaatgaaga gaaaattgct aaaatggcta taaaacaata
    12061 tggaactatt gagaagtttg ctaaagctat aaaaacaaat tttagcagtg atatattgaa
    12121 tttaggagaa aaatttgata gatataaaaa tgattgttta aaagaaaaac atccaaaatt
    12181 aaaagaatta tatagaaagc ttgtagaaga tttaagtagg aatccttctt caacagacct
    12241 tcaagaaata gcaaaagcga taacagatat atctaaaaaa gattatgaaa ttttctctat
    12301 ggatactggt gatgacaact ggtattatat ggttcaaaat tatctagtaa atcctatgtg
    12361 gatagaagaa gtagataaaa agtatggaag tggagcagga aagtttattg gacaagtact
    12421 aaagacttat ctaagagata gaaaaccaaa gataaataca ttgtatgaaa aacttgtaga
    12481 agatttgagt agagattgtt cttcaaggga agttcaaagc attgttgaag aaatagacaa
    12541 tgaaatgaaa agaagtaatg aattttataa aatagacaat ggtgaaagat actttgatta
    12601 tatgtcagag ctttatttac aagattctaa ctatataaaa gtaactgata agaattatgg
    12661 tgatggtgca tctaagttta ttggagaagc ttttaagatt tattttgata ataataattg
    12721 ttgaaaattt ggaagtaaaa aatatgtaag ttttataaaa gcacaaaaat aaggatgtag
    12781 attagctcca tctatatcct tatttaaagt tattaaaata tatagtttac atcaaactta
    12841 acactttcaa taatatttcc caaatatgtt tttatacttt caacttcttc taaagatttt
    12901 aatttatttt cagtataaat tgctttgtcc agaatattaa accttaatcc tatttcatca
    12961 aagtactcat gatttaaaac tttgtaccaa tggttttttt ctttattcca actcgtagat
    13021 attgaatcag cttctttttg agctttttcc caatcttcat tttctataga tttttctaaa
    13081 acagatatat tgtctttata tccttctgta aaatcatata gtttattgtt aacgtaaaat
    13141 ccaaataata caaataagat agtccatagt ataacaaatg ttgtagattt caatattaat
    13201 cacccttttt attttgaata aatattttgt cattttcatc taaaacacaa ataagaacat
    13261 ctttaaaagt ttctatgtga ttagatttta aaatacccat taaccagtta gtatcttttt
    13321 gagctatgtc tagattttta tttattattc taccatccag tataagtggg ataggtaaat
    13381 gattaaaagc tcttggagga gtactattgt agcttgatgc tggtacaaca cttagattac
    13441 catcagtctc caaaatagca tattgtacat cttttaggtt aaagtatcct tgaatcctaa
    13501 gttgttctaa taattcatct atactaattc tttctttttt aagctcttta tatacaattt
    13561 ttcctttatc tattaataca gaaggtttac ctgataaaaa tgaacttaat tttctgcttt
    13621 ttaatgataa gaatgaaatt agagtctgca tgattacaag tccagatatt gctgcaacac
    13681 catttatgat tggtatatta ttattttcca ttgggacaga tgcaacttct gcaattaata
    13741 gtgttataac taaatcaaaa cagttcattt cagctatttc accttttccc atgactctaa
    13801 gtgcaattag cactgttatg tataatataa tacttcttat taatactact gtcatatcta
    13861 tcaattactc ctttaaaaaa tatt
//
LOCUS       DAMKMI010000108        13837 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMKMI010000108 DAMKMI010000000
VERSION     DAMKMI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13837
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.112"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            221..361
                     /locus_tag="PC429_002776"
     CDS             221..361
                     /locus_tag="PC429_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848268.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(536..856)
                     /locus_tag="PC429_002777"
     CDS             complement(536..856)
                     /locus_tag="PC429_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF3848269.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(976..1713)
                     /locus_tag="PC429_002778"
     CDS             complement(976..1713)
                     /locus_tag="PC429_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF3848270.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1928..3751
                     /gene="acd"
                     /locus_tag="PC429_002779"
     CDS             1928..3751
                     /gene="acd"
                     /locus_tag="PC429_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF3848271.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3966..8264
                     /locus_tag="PC429_002780"
     CDS             3966..8264
                     /locus_tag="PC429_002780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF3848272.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8468..8929
                     /locus_tag="PC429_002781"
     CDS             8468..8929
                     /locus_tag="PC429_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF3848273.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8959..10113
                     /gene="nusA"
                     /locus_tag="PC429_002782"
     CDS             8959..10113
                     /gene="nusA"
                     /locus_tag="PC429_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF3848274.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10120..10401
                     /locus_tag="PC429_002783"
     CDS             10120..10401
                     /locus_tag="PC429_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF3848275.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10391..10702
                     /locus_tag="PC429_002784"
     CDS             10391..10702
                     /locus_tag="PC429_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF3848276.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10724..12664
                     /gene="infB"
                     /locus_tag="PC429_002785"
     CDS             10724..12664
                     /gene="infB"
                     /locus_tag="PC429_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF3848277.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12707..13087
                     /gene="rbfA"
                     /locus_tag="PC429_002786"
     CDS             12707..13087
                     /gene="rbfA"
                     /locus_tag="PC429_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF3848278.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13068..13745
                     /locus_tag="PC429_002787"
                     /pseudo
     CDS             13068..13745
                     /locus_tag="PC429_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5525 a         1569 c         2591 g         4152 t
ORIGIN      
        1 ttcattatat ctcaatttaa tatataaaaa agataataat agttagattt tttatatgaa
       61 ttttaatagg taaaatatcc tattgttttg tagatatatc ctaattatgt caaaaaataa
      121 aaaaattttt atttattaga gtttttggaa ataataataa cagatattat aattaattaa
      181 cagtttttgt aagtatttga caatagagag ggtgtaacaa atgggaataa tagagtcagc
      241 atcaaaattg gctgagatgg ttcatttatt ggctgttgaa aaggggataa ctgatataga
      301 agcatgggat gaagctgtga aagaatacag caaaatttat gaagaaagaa gaaatgaata
      361 gaatgataaa aaatgaaaac ttgatgataa aaaataactt tatttaaaga aattcaattt
      421 ttatagaagt attaaaaata atataaataa aaagatggta tttcaaatgt aagtaaaaaa
      481 tcattttggg ataccatcct tattatttat tttaataata tataagtaat aaatcttact
      541 tatccatatt ttttatgtaa cctctttttt ctaacttttt tatatgctcc ttacgtattt
      601 catcaagatt aataccatat tgttcttcta gcttaaacat catagtaaca catgtctgcg
      661 ctacatccat tagttcctta gcagttttct ttataatatc aacttcatct aaatcattat
      721 tttctccact tagatttctg aatttaccta tacattcagc taattcacct tgttcttcac
      781 ccattttgat aaatgtagat tctagtgttg ggtctaaatt attaagctct ggtaaagata
      841 tagtttttag tttcataaaa gctcctttca agttattcgt atttatacta aaacatcata
      901 acaaaaataa tactaaaaaa aaactttaaa ttcaacataa aaacttacat atataataaa
      961 aatcatgaaa taaatctata tttgtcttaa tacagcctca tataaaactt ctccaatagg
     1021 tttatttatt acaagagaag ctgacttatc atagactgta tttgctttat taataagagc
     1081 aatataatca cctttgtaat aatctataaa acttgctgct gggtacacag ctaatgatgt
     1141 acctcctata atcaataaat cagcattact tatagcagaa attgttttag taatgacatc
     1201 actgtcaaga gcttcctcat aaagtactac atctggtttt actatagaac cacagttgtc
     1261 acagtaagga atattacctc ccagatttaa catagattct aagtcaaaaa acttaccaca
     1321 tttagtacaa taatttctat gtacagaacc atgaagttct agcacatttt tacttccagc
     1381 catttgatga agtccatcaa tattttgagt tatgacagct ttaagcttac ccatttcttc
     1441 gagttttgct aaagctatat gagcattatt gggttttgca ttaggatata ttaatttatc
     1501 cttataaaat tcaaaaaatt cttctggata tctaacaaaa aaagtatgtg aaactaattg
     1561 ttcagcagta aattgtttat tgagtttttg gctaaataat ccagtagaac ttctaaagtc
     1621 tggaatatta gattcagttg aaacacctgc tccaccaaaa aatacaatat tattgtgatt
     1681 agctattaag tcttttaagc tatttgcatc catagttaca cctccaaatt attatatata
     1741 aattgtacta catattaggt cgtttatgtt ttattaattt aaataaatat atagtttaat
     1801 aagttaaata aagggaatat aaataaaatg tatacagagt ataaatagac aatatttact
     1861 aaaataacaa gttgtcgtat aattatatta aaattgtttt agtgtataag aagggcgggg
     1921 gaagtacgtg aagaaaaaag cagctttagc aacattggct atgttaccct taggtgtggt
     1981 aaatgcacat gctgatggag atataggtat agtgactata aattatttaa atgtaagaaa
     2041 tgaaccaact gccgaaagta gcatagcctt tgttgctaaa aaagatgata aagttttgat
     2101 taaagactct tctaatggat ggtataaaat aaaagctgaa tctggacaag aaggttgggc
     2161 ttcatcaaaa tatatagcaa aatcaaatag tgactcttta agaacgtcta caaataaaga
     2221 aaaacaagtt atttcaaata gtttaaatat gagaaatgga gcaggtacaa gttacagagt
     2281 aataactgta ctaaaaaaag gtcaaaaagt agaagtaata tcagagagta atggatggtc
     2341 taaaatcaaa tatgatggaa gactaggata tgtatctagc tcttatttag gagacgtttc
     2401 aaactcaact aataaatcaa aaactaaaca agtgaataca acttcactaa atgtaaggag
     2461 tggaccaaat acaagttatg gtttgttagg caagttgcca aaaggaagta aagtagaagt
     2521 aatatcagaa agtaatggat ggtccaaaat aaagtataat ggaaaagatg cctatgtatc
     2581 tagtatgtac ttatcggatg taagtcaaag taattcagac aattctagtc aaagcaatga
     2641 taaaaagaat actgataagg ttgtaaatac agcttcttta aatgtaagaa gtggaccagg
     2701 ttctacatat agtaagttgg gaaaagttta taaaggaagt aaagtaactg tactatcaga
     2761 aagtagtgga tgggctaaga ttaattttaa caataaagaa gcatttgtag taggcaatta
     2821 tttatctacc tcagcagata cttcaaataa taactcaaat agtaactctg ataacagttc
     2881 taatagtaat ggtaataatt cttcatcatc aggccaagta aatggtatgt caggtataag
     2941 tggggctaaa attgattata aatctttgag ttatacttta gaatctcata taagtaaaca
     3001 agttgagaaa gcagcatcag gaggaaatgt gatagctcca agtaatagga aaagtactcc
     3061 aagtcctgag tttagtactt tttcagctca aagaacaagc tcatttgtaa atgcaagttc
     3121 aagtgatata gaatattatt taaatcctaa gaattttaca aatactacta aaggtatgat
     3181 gcagttttta aagattaaca gttatagaga tggtatttca gaatcaagtc ttaactcgta
     3241 tcttaatggt ttatcttcta gtgtatttaa aaaccaggga gcagccttta taaatgctgc
     3301 caagaagtat aatatagatg ttgtatatct agtatctcat gctatgtggg aaactgctta
     3361 tggtaagtct acacttgccc aaggtcaaac attaacatct tataagggac aacctcttag
     3421 taagccagtt aaagtataca atttttttgg tataggagca atagataaaa gtgctaatgt
     3481 ttcaggtgca gaagcagcgt attccaatgg ctggactagt gtagaagcta caatagatgg
     3541 ctctgcaaag tggatatcac aaaattacgt aaatagttct aagtacaatc agaatactat
     3601 ctacaagatg aagtggaatt atgattatac atggcatcag tatgcaactg atgtaaactg
     3661 ggctaatgga atttctggaa ttatggaaaa tttaattggt ctttatggag gaggaagtag
     3721 tttggttttt gaagttcctc aatataagta atacataaat aaaaaatata gactatgggt
     3781 agttttcatt ttatttaaat tatctatagt ctttttgtat ataatttata atctgtaaat
     3841 ttaatgttaa caaaactttt atgttataat attaattcta agtaaatata agtgagtaat
     3901 aggtagaaat agataaaaag tggtaataat atatataaag ataattttga gaaaggagga
     3961 tatatatgga aagtattaag gaatatttgg ataagttaga aattaataat agtggtcttg
     4021 gaaagcaact taaagaagtt tatataaata gagtagttta ttttaaggaa gataagatag
     4081 tttattttta tctaacttct aaagatattg tttctcatga acttttagat aaatttaaag
     4141 aagaattgat gtataaactc gattatttta aagatatgag gatgaagata agatttactg
     4201 gtcttgaaag aaaatccaat aaagatgtta taaaaaaata ttggaataat atattgtata
     4261 tattaaagta tctttgtcca tctattgcag ggtggtataa acaagtagaa tttctttgtt
     4321 tagaagaaga attaaaaata aaacttccaa aaggtatatt ttatgagcga ttaatgaaaa
     4381 aaaatgtaac ttatgtactt aaaaccgtgc ttagcgaaga gttgggatta gatttaaata
     4441 taacaataga aaaagctgtt gatgaaaagg taaataaaga aagactcata agaatcaatg
     4501 atagagaaat ggaagaaaaa attagggctt tagaaattgg aaaagttaat aactgtgaag
     4561 aaaatgaaga ggaaagctat gtcattaagt ctgatgtaga tgaaaattta atctatggag
     4621 ataatgcaaa tgctatgatt gaaaatatag ttgaattaaa tgcatcttct ggaacagttg
     4681 cagttgttgg agatatattt gatgttgaca ctaaagaact taaaaacgga aaaatactta
     4741 tgatagcttc aattacagat tatactagct caataagctg taaacttttt ttaacagata
     4801 caaataagga tggagtactt gaaaatgtta aaaaaggagc atacttaaaa atcaagggtg
     4861 acatagttta tgatacatat caaagagaaa ttagtatgat gattagtggg ataagaaaag
     4921 aaactagaat agagagagaa gataaatcag aggaaaaaag agtagaactc catgcacata
     4981 cacaaatgtc ttctatggat gctatttgtt ctgttaaaaa gcttgtagag cgtgcagcca
     5041 aatggggtca ttctgctatt gctataacag accatggtgt tgtacaagga tttccagatg
     5101 ctatgaatgc aggtaagtca aataatataa aaatacttta tggagtagaa gggtacctgg
     5161 ttgaagatga ttctctcatt atagaggatg caaatgataa agaactttcg cagacatttg
     5221 ttgtatttga catagagaca actggctttt ctaatacaaa tgataaaata actgagattg
     5281 gtgctgtaaa aatagaaaac tttaagattg tagatagatt tagtgagtta ataaatcctg
     5341 aaaaagatat ttcttataag atacaggaat tgacaggaat aactaatgaa ttaattaaag
     5401 acaagccaac cattgaagaa gttcttccta agtttatgga atttgttgga gatagtgtat
     5461 tagtagcaca taatgcagag tttgatactg gatttatatc gcaaaaatgt agggaacaag
     5521 gcttgttata taataacaaa aaagtagata cacttatgtt agcaagagta atgttgccaa
     5581 acttaaaaag acatagatta aatgtcgttg caaaagaact tggtattcct cttttaaatc
     5641 accatagagc agtggatgat gctgaagcta cagcacttat atttaataag tttttgcaga
     5701 tgcttacaga aaaaggtgca aaaactctaa gtgatgtaaa caatatactt ggaaagattg
     5761 attacacaaa gcttagcaca aatcatataa ccttgatagc aaaaaattct ataggtataa
     5821 aaaatttata taaaataatt tcagatgcac atgtaaatca tttttttaga gcaccaagaa
     5881 tccttagaag tgtattaaat gaatataaag aaggattaat aattggttcg gcttgtgaag
     5941 ctggggtggt ttttcaagct gtaaagaaaa acgtgtctga tgaggaaatg aaaaaaataa
     6001 tagatttata cgattatatt gaagtaatgc ctatagataa taatagattt atgattgata
     6061 aaggagaagt aaaggacgaa gaagagttaa gggagttgaa taggaaacta attgatacag
     6121 ccaaaaaatt tgacaaaatt ccagtagcta ctggggatgt tcatttttta gacaaacatg
     6181 aagctgtctt aagaaaagtt cttaaatatt ctcaaggttt taaagtagat gaagaagaaa
     6241 cttatttaca ttttagaaca acagatgaaa tgttggaaga gtttagttat cttggagaag
     6301 aattggcata tgaagttgta gttgaaaata gtaatctgat tgccgatatg gttgaagata
     6361 taaaacctat accagatgat acttatcctc caattataga gggctcagat gtggagttaa
     6421 gagaaatgtg ttacaaaaaa gccaaacgaa tctatggaga cccaatacca gaaatagttc
     6481 aaaaaagact ggatagagaa ttaaattcaa taataagcaa tgggtatgca gttatgtata
     6541 ttatagctca aaaactggtt gcaaaatctc tttcagatgg atatttagtt ggttcaagag
     6601 gctcagttgg ctcatctttt gcagctacta tgagtgatat aacagaagtt aatcctcttc
     6661 cagcacatta tatttgtgaa aacgaagact gtaaatattc attcttttat gaaataggtg
     6721 aatggggctc aggtgttgat ttaccagaca aggattgtcc aaagtgtgga agaaaacttg
     6781 ctaaaaacgg tcatgatatt ccatttgagg ttttcttagg ttttgaaggt gataaggaac
     6841 cagatataga cttaaatttc tcaggaaatt atcagccaac aattcataaa tacacagaag
     6901 aactatttgg agaggggtat gtatataggg ctgggactat aggtactgta gcagaaaaaa
     6961 cagcttttgg atacgcaaga aaatatgtgg aagaaaataa tataagtgta ccaaatgcag
     7021 aagtacttag gctttcaaat ggatgtactg gagtaaaaag aacctcagga caacatcctg
     7081 gtggagttat ggttatacca gactataaag atgtatacga ttttacacct atacagtatc
     7141 cagcaaatga tacttcttgt ggtgttataa ctactcactt tgattatcat tcaataagtg
     7201 gtagaatact taaacttgat atacttggac acgacggtcc taccattata aggatgcttg
     7261 aagatatgac tggaataaat ataacagaaa ttcctttaga tgataaagag actatgtctc
     7321 tatttacatc tacagaagca ttaggtgtta caccagaaga aataaactgt cctataggtt
     7381 gccttgctgt accagagttt ggaactaagt ttgtacgaca aatgttactt gacacaaaac
     7441 ctactacatt tgcagaactt gtacgtatat ctggtctctc tcatgggaca gatgtttggg
     7501 taaataatgc acaagactta gttagagaag atatagttgg attaaaagat gttatatcta
     7561 cacgagatga cataatgaac tatttaatat ttaaaggatt acctcccaaa atgagcttta
     7621 caataatgga gagtgtaaga aaaggtaaag gtttaaaacc agaacacatt gaagaaatga
     7681 aaaaaaataa tgttccagaa tggtatatag gctcatgtaa aaagattaag tacatgttcc
     7741 caaaagcaca tgctgttgct tatgtaatga catcatttag aattgcatat tgtaaagttc
     7801 actatccaga agcattttat gcgacttatt ttacgacaaa agttgaggat tttgatgcag
     7861 atttaattgt taaaggatta gatgctataa aatcaaaaat aaatgaaatt gagtcactgg
     7921 gaaatgatgc aaccacaaaa gaaaaaggta tgttgaccgt tttagaagtt gctcttgaaa
     7981 tgtatgctag aggaataaaa ttattaccag ttgatatata taaatcagat gcaactgagt
     8041 ttatagttgt aggagaaaaa acattacttc caccaatggc agcaatacaa ggtcttggag
     8101 gaaatgcggc tataaacatc caaaatgaaa gaaaaaatgg tgaatttata tcaaaagaag
     8161 atttgagaaa gagaacaaaa atatcaaaaa cggtaataga aactcttact aatcatggtt
     8221 cacttgagaa tatgagtgag agaaatcaat tgtcattgtt ttaagttagc ccaaaaagtt
     8281 gcaaataaaa gaattttatg atatcatagt atagaaaata gtatactttg cagaaactaa
     8341 gagtgagaca gctgttctca ctcttttatt ttaaatatat tattaaaaag ctggtttatt
     8401 tttattaaat atattttatt taagattata taaactggtc atttacaatt gaataggagg
     8461 aactttaatg aaaaaaaatt tagaagctac aattgaggag attgtaacta aaataacgga
     8521 tgaacatgga tttgaaatgg ttgatgttga atatgtaaaa gaagcaggag aatactactt
     8581 aagagtatac atagataaag aagaaggaat atctttaaat gaatgtgaat tagttagtag
     8641 agaattaagt ccaatactag atgaaaaaga ccctataaaa gaaaattact ttttagaggt
     8701 atcttctcca gggcttgata gagcattaaa aaaagacaga gattttgtta gatatcaagg
     8761 tagagatgta gatttaaaat tatataaacc attaaatgga tgtaagcaat ttgaaggtga
     8821 acttgtagga ttaacagaag ataataatat aaaaatcatt gcaaatggta aggaaatgga
     8881 atttaataga aaagatgttg ctattgtcag attagcaata aagttttagt ggtatattaa
     8941 atagggagga tataaaaaat gaatcatgaa tttatggaag cattagatga attagtagtt
     9001 gacagaggta tagataaaga aatacttata gatactatag aacaagctct tttaactgca
     9061 tacaagaaaa actttggttc agcacaaaat gttagagttg aatttgatag agacaaggga
     9121 gatataaaag tattttctca aagagttgtt gttgatgaat cagatttata tgatactttc
     9181 ttagaaatag agttagcaga tgctagagag ataaatccta attatgaatt aggtgatatt
     9241 atagaacatg aagttacacc taaaaacttt ggaagaattg cagctcaaac agcaaagcaa
     9301 gtagttgttc aaagaataag ggaagctgag agagaaatag tgtataatga atttatagaa
     9361 aaagaaaatg aaatagttac aggagaggtt gctagagcaa ataaaaatgt tgtttatgta
     9421 aatcttggaa gaatagaagg tataatgact caaagtgaac aaataccatt tgagaattat
     9481 aaagcaggtc aaaaaataaa attctatata cttgaagtta aaaagactaa caaaggacct
     9541 caaatagttg tatcaagaag tcatccgggt ctagtaaaga gattgtttga agaagaagtt
     9601 cctgaaatat atgaaggaat agttcaaata aaatctgtag caagagaagc agggtcaaga
     9661 actaaaatgg cagtaagatc tatagatgaa aaaatagacc caattggagc ttgtgttgga
     9721 ccaaaaggac ttagagtaaa aaatatagtg gatgaacttg gcgatgagaa aatagatata
     9781 ataaaatata gtgaagaccc agcagagttt atatcagcag cacttagccc atcaaaggtt
     9841 gttagagtag atatagatga agatgaaaag tctgccttag taatcgttcc agattatcag
     9901 ttatcgcttg ctataggtaa agaaggtcaa aatgcaagac tagcagcaaa attgactaac
     9961 tggaaaatag atattaagag cgaatctcaa gcagaacaag aaatgttaaa tcttgaaaat
    10021 tccaaagaaa tagaagctga ggatgttgaa gctgaattta tcgaccaaga agagttagat
    10081 acttcagagg ataattttag tgaagaagag taaggaggga tgattttgaa aaaagtaaaa
    10141 aaagtgcctc aaagaaaatg catagcatgt caagatagag actctaaaaa agagcttata
    10201 agaatagtta aaaataaaga aggaaaaata tttttagacc caacaggaag agccaatgga
    10261 agaggagcat atgtatgtaa aagctctgaa tgtctaaaaa aagctataaa gagtaaagca
    10321 ttaaataaag cttttaaaat agatgttcct gatgaagtct atgataatct acttatggag
    10381 ttagaagaat atgaaaaata ataaagaaaa agtactttcg ttcttagggc tcgcaactcg
    10441 tgcagggaaa atagtgtctg gtgatgattc aactttgctt gaccttaaaa aaggaaaagt
    10501 aaatttgatt ttaatcgcag aagatgcatc aaataatact aaaaaacttt ttaaagataa
    10561 atcaactttt agaaatattc catatctatt tttttcaaca aaagaggaaa taggatttgc
    10621 aataggaaaa tctccaagag cagttgtagg aataaaagat gaaaattttt ctaaaaagat
    10681 aattgaacta atagaaattt aaaataaaat agggggtgat cttgtgtcaa aaacaagagt
    10741 ataccaaata gcagaagaat taaatatatc aaatgaagag cttataaata agttagctga
    10801 attagatata aatgtaactg acaaagattc agtattagaa ggtgaagagt tagaattagc
    10861 acttgaaatg ttaggtgaag atttatctca agaaaatgga aatgttatag aaatagatgg
    10921 aaagttgaca gtacaagtat tagcaacgaa attagataaa tctccatcag aaataattat
    10981 gaagcttatg aaaatgggaa ctatggcaac aataaatcaa gagataagtt ttgagattgc
    11041 tgctttggct gccaaagact atggatttga attaacagta gctgaaagtg atgatacaga
    11101 agctttagaa atagaagctt taatggaaat tgaagaagac aaagaagagg atttaaaacc
    11161 tagaccacca gttgttacag ttatgggaca tgttgaccat ggtaagacat ctttactaga
    11221 tgcaataaga aaaactgatg tgatctcagg tgaagctgga ggaataactc agcatatagg
    11281 tgcttcagaa gttaaaatca atggacacaa aatagtattt ttagatactc caggacatga
    11341 ggctttcaca tcaatgagag caagaggagc acaagttaca gatatagcaa tattagtagt
    11401 tgcagcagat gatggtataa tgcctcaaac agttgaagct ataaaccatg ctaaagcagc
    11461 aggtgtacct ttaatagttg ctattaataa aatagataaa ccaggtgcaa acccagataa
    11521 ggttaaacag gaattagcag accaaggatt attagttgaa gactggggtg gagaagtaat
    11581 agcagttcca gtatcagcta agaaaaaaga aggaatagat acacttttag aaatggtatt
    11641 attggttgct gaaatggaag agttaagagc taatccaaat aaaagagcag taggaactgt
    11701 tatagaagct gagcttgata aaggaagagg gccagttgca acagtacttg ttcaaggtgg
    11761 tactttaaca gtaggtgacc caatcgttgc aggtgttgca tgtggtaaag ttagagcaat
    11821 gataaatgca aaaggtaaga gagtaaaaac agcaggacca tctacagcag tcgagatatt
    11881 aggtctatca gaagttccac aaggaggaga ccaatttgtc gaagttccaa ctgataagat
    11941 tgctagaagt gtagcagcaa gaagacaaca aatagttaga gatgaaatgt taaaatcaac
    12001 tcaaagatta tcactagatg ctctatttag tcaaatgagt gaaggttcta taaaagacct
    12061 taatatagtt ataaaagcag atgtacaagg ttcagtacag gcagttaaac aatctttaga
    12121 aaaattatca aatgaagaag ttcaagttaa agtaatacat ggtggtgttg gagctgtaac
    12181 agaatcagac atattacttg cagcagcttc taacgcaata ataataggat tcaatgtaag
    12241 accagttcca ggtgcagaat ctctaggaga aaaagaaaat gttgatataa gaacatatac
    12301 tataatatac aaggcaatag aagatataca agcggctatg actggtatgt tagaccctga
    12361 atatgttgat gaagaaacag gaaaagctga aataagagaa atatataaaa tatctggtgt
    12421 tggaacagta gctggatgtt atgtgactaa tggtaagata tttagaaatt gtaaagtaag
    12481 attagttaga gatagcataa taatacatga aggtgagctt gcagcactta agagatttaa
    12541 agatgatgta aaagaagtta attctggata cgaatgtggt atgagttttg taaattataa
    12601 cgacataaaa gaaggcgata tagtagaagc ttatataact aaagaagtag aaagaaaatt
    12661 ataaattcgt atcaaagaaa atcagttagt aaagaaggtc gatggtatgg catcatataa
    12721 tagaacaaga aggatagcag aagaaataag aaaagtcgta agtactatgc ttataaatgg
    12781 agtaaaagac cctagaataa catctatggt gagtgtaact gatgtagaag ttacaaatga
    12841 cctaagatat gcttatgtat atgtaagtat tctaggcgga gatgaagaat ccacactaac
    12901 aggtcttaaa agtgctggag gatatataag aagagaagta ggaaaaaata taaaacttag
    12961 atatattcct gagatagtat ttaaacttga tgattcaata gaaaaaggaa tgtatatgga
    13021 tagccttata aaaagagtta atgaaaaaaa tgctcaacaa aatgaggatg aaaattatga
    13081 tgaataatat ggatagtatt ataaactata tttatgaggg tgactatttt atagttacct
    13141 ctcatatcag tccagatgga gacaatattg gttctacact ttcaatgtat tatacattga
    13201 agaatttagg aaaaaatgta tattatgtac ttgatgatga agctccatta aatttaaggt
    13261 ttttggttga aggtgtaaaa atctataagt ctaatgaatt taatatgaag aactattctc
    13321 taattgcact tgattgtggg gataagatga gaatatgtgt gagtgatgaa atcaaaaata
    13381 atgcatcaaa attaatctgt attgaccatc atgctagtaa tgattcatat ggagatttaa
    13441 attatgtaga tattgatgca tcttctacat gtgaattagt ttataattta ttagttagat
    13501 ttcagcaaat aaaagatatt aatattataa atgaagatat agcaacatgt ttatatacag
    13561 gtcttgtaac tgatactgga aacttttcgt attcaaatgt acatgcaagt agctttgaaa
    13621 tggcaaaaaa tttacttgtt ttaggtgcac aaaaaaatac tataatacaa aatatatatc
    13681 aaagcaattc ttctggttat tacaaattat taggagaagc tctaaagggt ttggagatat
    13741 ttgattaaaa aatagttacg cacactcgtg actttagtca tgagttagta gctaaaatag
    13801 tcagcatata gggaaactta tatgtagaga taggata
//
LOCUS       DAMKMI010000109        13758 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMKMI010000109 DAMKMI010000000
VERSION     DAMKMI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.113"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            114..1907
                     /locus_tag="PC429_002788"
     CDS             114..1907
                     /locus_tag="PC429_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF3848279.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(1988..3370)
                     /locus_tag="PC429_002789"
     CDS             complement(1988..3370)
                     /locus_tag="PC429_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF3848280.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            3628..3975
                     /locus_tag="PC429_002790"
     CDS             3628..3975
                     /locus_tag="PC429_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF3848281.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            3993..5027
                     /locus_tag="PC429_002791"
     CDS             3993..5027
                     /locus_tag="PC429_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848282.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(5076..6125)
                     /locus_tag="PC429_002792"
     CDS             complement(5076..6125)
                     /locus_tag="PC429_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF3848283.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            6400..7935
                     /locus_tag="PC429_002793"
     CDS             6400..7935
                     /locus_tag="PC429_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848284.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            complement(8120..8806)
                     /locus_tag="PC429_002794"
     CDS             complement(8120..8806)
                     /locus_tag="PC429_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF3848285.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            8931..10313
                     /locus_tag="PC429_002795"
     CDS             8931..10313
                     /locus_tag="PC429_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3848286.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            10715..11983
                     /gene="eam"
                     /locus_tag="PC429_002796"
     CDS             10715..11983
                     /gene="eam"
                     /locus_tag="PC429_002796"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF3848287.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(12076..12432)
                     /locus_tag="PC429_002797"
     CDS             complement(12076..12432)
                     /locus_tag="PC429_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848288.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(12583..13677)
                     /locus_tag="PC429_002798"
     CDS             complement(12583..13677)
                     /locus_tag="PC429_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF3848289.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
BASE COUNT         5388 a         1731 c         2177 g         4462 t
ORIGIN      
        1 tgataattag agtgtataag atttatataa attatacaaa aaatatttat gtaataaact
       61 ttacatactt atatatttag ttattcaaac tattatttcg aggaggtctt gttgtgaata
      121 taaaagatag attaagtgga ttaagaaaat ttatggaaga aaaaaatata gatgcctata
      181 tgattccatc ttctgataat caccaaagtg aatatgtagg agattacttc aaatcaagag
      241 aatttatatc agggtttaat ggttcagcag ggacggttat tgtgactaaa gacgaagctg
      301 ggttatggac agatggaaga tattttatac aggcagaatc tcaattagaa ggaagtacaa
      361 taaaattatt caaaatggga caagaaggtt gcccaactac agatgaatat ttatataaaa
      421 atattcctga aggaggaact ttaggatttg atggtagagt tatatctgct agagaaggag
      481 ctacattagc tgaaaaatta tctaaaaaag gtataaagat agaatatcag tatgatttaa
      541 tagatagtat atggccagat agacctgctt tatcagatag taaagcattt ttattagatg
      601 ttaaatattg tggagaaagt tttagctcaa aattagctag attaagagaa aaaatgagtg
      661 aaaaaggtac ttctactcat gtaataacta ctcttgatga tatagcatgg ttattcaata
      721 taagaggtgg agatgttaaa tataatccag ttgtactttc ttatgcagta ataactttaa
      781 aagaagttta tctatttgtt gatgaaagta agttaaatga agaaatacta aatgaactag
      841 ctaaggaaaa tgttcaaata aagccatata atgatgtgta tgaatttgta aaaaatatag
      901 acaaaactga aaaagtttta ttagatggaa ctaagcttag ttatacaatt tacaataata
      961 taccttgtga agttgagaaa gttgatgagt tcaatccagt aatgtttttt aaagctcaaa
     1021 aaaatgaggt agaattagaa aatataagaa attcacatgt aaaagatggt gtagctttca
     1081 ctaaatttat gtactggtta aagaaaaatg taggtaagat ggaaatcact gaaataagtg
     1141 ctactcaaaa attagaggac ttaagaagag agcaagaagg tttctttgag ccaagtttca
     1201 atacaattgc tgcatataaa gagcatgctg caatgatgca ctatagtgca actccagagt
     1261 caaattataa attagaggca gaaggattat ttttagtaga ttcaggtgga caatattatg
     1321 atggaactac tgatataact aggactacag ttttaggacc tataagtgat gaattaaaac
     1381 tacactttac ttctgtagca agaggaatga taaatctttc taaggctaag ttcttgcatg
     1441 gttgtagagg atataactta gacatactat ctagaagttg catgtggaat atgggaattg
     1501 attatcaatg tggaactgga catggtatag gatttgtgct aaatgttcac gaagctccaa
     1561 atggatttag atggagagta gtacctgaaa gatttgatag tgcagttctt gaagaaggaa
     1621 tggtaactac taatgaacca ggtatatata tagaaggttc tcatggtata agaacagaga
     1681 atgaaattgt agttagaaaa gctgaaaaga atttctatgg tcaatttatg gaatttgaag
     1741 ttgttacgtt agctcctatt gacttagatg gtatagtacc agaattaatg aataaggatg
     1801 aaaaagatta tttaaactgg tatcataagt tagtttatga taaaatatca cctttcttaa
     1861 ctgatgaaga aagagaatgg ttaaaagttt acactagagc tatatagttt gtactagata
     1921 caataaaaat cgaggtatta taatgcctcg attttttatt gtatatttta ttaactttaa
     1981 aaaattttta atttgataat tctactaatt ttttattcat tataagtagt ataattccaa
     2041 atatcaataa aaatgatggt atcactatga agacttgtaa cataccaagt tggtctataa
     2101 tagcttgcgt atagccagat aatttatttg ctgcaaattt gcctaaaaac caaacaccca
     2161 ttaggaatga aatatacttt tcaggcgaaa gtttatttac cataccatac ccgactggag
     2221 aaaagcaaat ttctcctata ctttggaatg ttaaaaatcc aactacccaa aatacacttg
     2281 ctttgtgaat tgaattaggg tctgttcctg tttgtataac tgctactatc ataaacaaaa
     2341 atgctatggc taatagtata aaacctaagc tcattttttt tggcacactt aaatcgcctt
     2401 ttttagtttg agataatttt acccatacta aagcagagat aggtccaaga ataacacaaa
     2461 ggagaccatt gtatgaatct atccatgaag taggaatttg aaaattgcca acagtaagat
     2521 ttatgtgttc ctttgtatag agtgcaatag acatagaggc ttggttatat gctatccaaa
     2581 aaaatactgt gaaaaaagct aacaccaata ttactattat tctttttttc tcaatcaaag
     2641 ttaaaggtct tgaagctagc attttcttat attctagtga ttttcttata gactctctcc
     2701 cgttactagg gtcattattc aaaggtttat aaaactttct gtttccaaat ataaagaaga
     2761 ataaacctat tagaaccgta attgatgaaa ataaaaatac atatctatat ccatacatac
     2821 ttatagataa tccgcttttc atagcaaacc attcattagc aactaaaccg catattaaag
     2881 ggccaaataa ggtacctaga ttagtagcca tatacgtaat agagaaagct ccatcttttc
     2941 ttgggtcatc attggtatat aagtttccaa ccatagtatg taagtttcct ttataaaaac
     3001 ctattcctaa tccagataaa aatatcatga tgtttatatc catcttacta tttgctatgt
     3061 atcctagaaa ataaccaatt gaaaccatta gcattccaag tataatacag tttttgggac
     3121 ctaaaaatct atcactgacc catccaccaa taactggaga gaaataagtc catgctaaaa
     3181 aagatgcaag cattgatgcg gattctgctg gtgttaaccc taaaccacct ttggagactt
     3241 gttcagctaa aaacaataat aataccaatt tcataccata gtatgcaagt cctacaaagg
     3301 taaatattga acagcagaaa tataaatctc taggttgttt aagtttttct tgcgttttta
     3361 aaactgacat atgtcgagaa cctccagata aatagtttaa caaaatttta ccatactata
     3421 tgagtatatg tttttttcat atagacgtca acttgtaaga taatttaatt aagtcgaaat
     3481 atatgtaaaa atagtatcat ttttgtgtat tcagtaatat ttttttatga taatatatgt
     3541 tattattata ttaaaataca aacatattta tggaaaggat atgccaattt agtagttaga
     3601 ttgattgttt ggcaggtggg atgagctatg ataagaaaga aatctgaatt acaagtagaa
     3661 gtaaaagaaa atttaaatgg tggtatagga aaggttaagc ttgaaaacat aatacaaaat
     3721 gaagagctta aaggaaaagg aagactattt aaaagagtag ctttaccagt aggaagttca
     3781 attggtgtgc atgaccatac aacagatttt gaagtatatt atatactaaa gggaaaaggt
     3841 aaagtatttg ataatgggga atttgtagaa gtaaatgagg gtgatgtagt gtatacagct
     3901 gatggcgaaa aacattctat agaaaatata ggagaagaag atcttgagtt tgtagcacta
     3961 gttttatata tgtaaaatat ggaggaggag tcatgacagg caagatagaa gaaattaagg
     4021 acaagataaa tagattttgc aactccaatt taaatcaaga atacaaagat ataagcttta
     4081 aaattttgca aaacctttta gataataata aagagataat tcattcttct agagctgata
     4141 tatggtcttc agctatacta aatatagttt tagaacaaaa tcttttatac aataagaagc
     4201 atccactaca tataacaaaa aaagagtttt caaaacaaat aggtgtttct ttaaatacta
     4261 taaataataa atcgtcttta ataaaagatg tttgtagtat tgacttttta aatcaagata
     4321 ctatagctat ttctaatgta gaattttggg taaaagagac caattccaaa tcaaagtctg
     4381 tagatttaga tttagaaaag aaaaaaatac tatataaaag atatattaag ctatcacaag
     4441 atgcgaatag tgatattgaa tcaattagat atatggagga agctgtaaat ataggtaaaa
     4501 gtatgattac taaagaagat aaagaacttg gattttggaa aggtttatct accagagcct
     4561 atatgatagc acttgaatcc cttgctagaa agttagaaag tattaataat ctaaaggagt
     4621 ccaaaaaagt attagaatat ttaatagaaa ttaatcctag tgatgagcag ggaataagat
     4681 acaaattatt aaatgtatta attagattaa atgatagaac ttcaataaat aaattatttg
     4741 aactttataa agaagaaaaa tctgctacat ggatgtacag taaagctctt tattacttta
     4801 aaaataaaag tatgtttttg gcaaatgatt ctattaaggt tgctagaagt aaaaataagt
     4861 acgttggatt atatttgtta gattggcgaa atgcttttgg tagagaattt gttacagagg
     4921 aagaaaaggc agaagctgtc tattactatg atgaaaacat agtgatttgg aatgaagtta
     4981 aaggttctat ggattggcta ttaaaaaaaa tgttggaatt ttcataaaaa aaactactcc
     5041 ttacttttat ataagtaaaa ggagtagttt ttaatttata catctagttt taaatcttca
     5101 ggttttatta gacctttttt tctcataaat tctgaaatca acaaagttac aatagcagga
     5161 agtataaaat taagtaaaag tataccaacc cacatagata tacctccttt tccagcactt
     5221 tgcatagcag ttactgttcc aaattgtcca accaatccac aagtacccat accagaacca
     5281 ataggaatat tctccatttt gaatattgtt gtagataatg gtccaagtaa tattgaagct
     5341 acagtaggtg gtatccaaat tttccaattt tttattatat taggtacttg caacatagat
     5401 gtcccaagac cttgtgccac aaggccgcca actccatttt ctctaaagct cataacagca
     5461 aagcctacca tttggcaaca acaacctaca gttgctgcac caccagcaag accacctaaa
     5521 cttaacatca tacaaatagc tgcactactt ataggtaatg taagagctat tccaacaagt
     5581 gcagagacta aagctcccat tattataggt tgcatagttg tagcttgcat tattatattt
     5641 ccaaatgaag tcataaaagc agaaacagct ggaccaataa gttcagctac aagtactcca
     5701 attagtattg ttacagcagg tgtaaccaat atatctattt ttgtttcttt tgaaactaat
     5761 tttccaacct ctacagcaaa aagtgtagcg acaaatgctc caacaggtcc tcctaattca
     5821 ttaccacaag ctccaacaag tgcacaagaa aatagtacaa gtggaggtgc ttgaagtcca
     5881 tacgcaatag ccactgctat agctggacca gtaacctgtt tagcaagagg gcttataact
     5941 tcagtaaaaa gagaaatatt gaaattatct cctaatgtat tgaaaattgt tccaattaaa
     6001 agggacgaaa aaagaccgag tgccatataa cttagtgcgt caataagata cctttgaaca
     6061 gaaatttcaa tattttttct tttagcaaat gatttgaaat ttcttttttt ataattgatt
     6121 tccaatttaa aatcctcctc atataatata agtataattt ctttttaatt aattatataa
     6181 cttttcagct gacaagacaa atgttatttc actaaaatta gactattgta atagtaagaa
     6241 attaacatta attttagttt taaattttaa atttatggtt tataattagt atagggatat
     6301 tctgaaatat aataattttc cagtttatcc caatatttaa cttaggtaga ttgcttacaa
     6361 aacaaatgat tatatattag gctaaaagga agtggataga tgatatttgt aaaaaataag
     6421 aaatacagaa aaattgccat aattatatct attcctatat tgcttgttgc gcagtactac
     6481 ctttttaaat ttggaatact tagaccaatg gtaaagggag tagaaattga aattgtagat
     6541 ggggaatatg ttaaagatat agataaatat gtagtaaaat taaatgaaac agttactttg
     6601 tctacaggtg aatatattaa ggttccttcg tatgctaaaa atccaaatat ttggtttaat
     6661 gtattggata actcagggac tttaaaaata gatggaaata aaatgacagc attaaaggtt
     6721 gggtattctt ctgtgggtat catgaaaaat tcaagagtat taaaaaaggc tactataaaa
     6781 gtagtagacc cagaggtaga atcactagat atggagatta atggggattt aaaacatgtt
     6841 ggtgatagtg ctactataga aagtacaata gaagttgatt atgataagtt taaaaaatca
     6901 tataagccag tttataagag tagcaatgaa aatattttaa aaatcaaagg aaataaagta
     6961 agtgcagttg gagttggtaa agctacaata tatgctaagt gtggaaataa agaaattgag
     7021 agaacattta ggattcaagc tagagttgct gaaataaata tggatgatat aaaaatagaa
     7081 gttgaccaga atgaaaaatt gaaaccagat ataataactt ctccacctgg tttagaacca
     7141 ccaaaaatat attatgagtt agtagattca aaattacctg tagaaagagc aataagttta
     7201 agtagtaatg gaaatgttgt agggcttaga gaaggtaaag atagggtgag aataacttgt
     7261 ggagataaat ctaaagtaat aacagtaaca gtagtcaaag aaactataac gaatgactat
     7321 atacaaaact tggttggttc acataaaata gtaaacaata aagttattat taaacttgaa
     7381 tgggattata tgaaagatgt ctttgattat gatatatatc ttaaagataa gttgtcagga
     7441 gaatcattta agaaaattaa atctataact ataaatgaaa gtgatttagg taagtctaac
     7501 aaggtatatg caactataga agtagatatg aagggtaatc aaaatataga ttttgatatt
     7561 tatgtcatag gtaaaaagga ttcagataca actaagccaa gtaatgtaat aaatattacc
     7621 aatggaaatg gaaattcaaa taatgataat aatattaaag atatgagggt agaaaatata
     7681 tcagctaatg ttgatagaga taataatgtc attagagtta actggagtag tatatctcat
     7741 aaggactgta cttacagtat atatgtaaaa aataatacta atggagatgg agaatttact
     7801 ctatatcaaa gtgatataca gggaaatgag catactataa gtattcctga aggagattta
     7861 aatctagata tttatgttgt tgcaaattat aatggaaata gctctcaaaa tagtgatgtt
     7921 ataaatgtaa gatagatttg tatacaaatt gaaagtgttg agtttattta aatattaaat
     7981 taagaaaaag gatagtatag ggtaattata ctatcctttt ttgttacaat atataggttt
     8041 aaaatctcta gtaatataaa ttataaaaag tcttatttac acaaatctat agatattttt
     8101 tttattaatc aatattgtct taaattgaac ttagtgcggc ttctattgca tgtattactg
     8161 tcgtatcaaa tagtggaata gaagtgtcat tttgtttaat taaaagacct atttctgtac
     8221 atcctaatat aacagcctct gcaccttgtt tgccaagctt atctataata gataagaaag
     8281 ctttttttga agattcagat ataattccta agcaaagttc attaaatatt gtgtcattta
     8341 ctattatacg gtcttcttca tttggaatta agacttcaat tccattatta ataattacat
     8401 ttttataaaa atcttgttcc attgtatatt tagtacctaa taaggccact ttttttattt
     8461 ctttttccct taataccgta gcagttacat ctgctatgtg aagtaatgga atatgtatac
     8521 tttcttgtat tttatctgat accttatgca tagtattagt acagataaca ataaaatctg
     8581 cccctgcctc ttgtaattta attgcagcat cagctaaaat tttggcgctt ttttcccaat
     8641 ttccactaga ttggcattct tcaatttctt gaaaatctac actgtaaagt atacatttag
     8701 aagagtgtag tccacctaat ctttctttta ttactgtatt aataacttga taatatgtaa
     8761 tggtactttc ccaactcata ccaccaatta agcctatagt cttcatatta tacctctctt
     8821 atttatttat tttgaaatag ctatttacaa atacaagtat agcatctaaa atagaagcat
     8881 acaataataa aattgtatgg agtacaatta taattaagag gagtaattat atgccaataa
     8941 attcttttga aaactatcct atgacatgga aaccaaagtt agataaccga aaaccaccaa
     9001 tatataaaac tttagcaatg ttattagaag aagatattaa aagaggaaat ttaaatcctg
     9061 gagataaatt gcctccacaa cgggaattag cagattttct agatttaaac ttaagtacaa
     9121 taacacgagc atttaaactt tgcgaagaaa agggactaat atgtgcaaaa gtgggaaagg
     9181 gaacattcat ttcttctgat gtaaatgtaa gtaatacatt attatatcaa acggaatcaa
     9241 aagacataat tgagttgggg acagttcatc ctccgtatga gcaaaataca tatattattg
     9301 actttattaa aaatgtacta aaacaaccag aaatggatag gtttttacaa tacatgtctc
     9361 catctggcac atatatgcaa aaaaagtcaa ttgcaaaatg gatagaaaga aataatgtat
     9421 acaccagtga agaaaatata ttattatcta caggagggca aaatgctata tgtgcaactt
     9481 tactaggatt atttaaagct ggtgatagaa ttgctacaga ttctttaagt ttttctggaa
     9541 ttaagtcaat agctaaaatg ataggtattc aattagtccc aattcctcaa gaaaataatg
     9601 aaatatcgat tgaatatctt gaaagttatt gtaaaaatga aaatataaaa ggcatatatc
     9661 ttataccaga ttatcataat ccaacgacac atactatgtc tgatttatct cgtaaaaaga
     9721 tagccaagat agcaaagcaa tataatataa taattattga agatgctata aatagcattt
     9781 ttagagatgg tatacaaact cccatatttt ctttagctag tgataatact atatatattt
     9841 ttagtacatc taaattttta tgtgctggtc ttagagtagc atttgtagtt gctccaaaac
     9901 gatatataga aaatttagaa aatgcactat ataatatgaa tctaatggtt tctccattta
     9961 cagcagaaat agtacaccgt ttattatatt ctcctattat agataaaatt atagaagaga
    10021 aaagagatgc aattatagaa agaaatgaag ttgctgataa agttttgtca gattataatt
    10081 tgattggtga taagaattgc agttttcgtt ggttacttct tcctgatgaa ttggatggaa
    10141 aatcatttga aatttctgca aaaaatctag gagttcaagt atattgtgcc gaacgatttt
    10201 cagtaggaaa ctctacagtt ccaaaagcag ttcgtatttg tgtaacagct ccaaaagatg
    10261 tagaagaatt agaaaaaggg cttaatataa taaagtcact tcttaagagt taaatttatt
    10321 ttactattat ttaattaaat aaataaaaag gcggagaaat ttattataat aaacctccgc
    10381 ttttattatt taatgaaata ctttactttt tattaaaaaa tttaatttta ataatcttga
    10441 aaatataatc gttttatggt aaaataattc ttaaaaatag aaaaaattaa tcatttgtta
    10501 aaaaaaatgc ataatttatt gtaatttatt gacgaaggtt gaaaaatatt cataaatgtg
    10561 cgaaataaca gaaaaaatgc aaaaattaat tcaatttcat ttgttataaa atctatatgg
    10621 caaaattaaa tctgcctatc agaatgatat aaacacatca gaaaggagga cgctatcaaa
    10681 taatacaagt agttaacttt gatagcaaaa aattatgaat gaacaaacta gaatatcctt
    10741 agagagagct gctgaattaa aatcaaaaat tgatgattat attcaggcta gaaaaacgat
    10801 taacagaggt cttgaaaaag aagaagagat aaataaacga aaacagaaaa tattaagtat
    10861 cttaaatgga actgaagagg attggaataa ctacaaatgg caattatcaa atagaataac
    10921 agatgtagat actttatcaa aaattataac tctaactaaa aaagaaaaag aatatataaa
    10981 agaggttggt actcaattta gatgggcaat atctccatat tatttgagtc ttatagaccc
    11041 agaagatata tgtgacccaa taaaattact gtctatacca acacatatag agttggaaga
    11101 tgagcaagaa gatttggacc caatgggaga agagtataca aacccagcag gatgtataac
    11161 tagaagatac ccggatagat taataataaa tgtaacaaat gagtgtgcta tgtattgtag
    11221 acactgtcag agaagaagaa atattggaca acaagattct cataagtcaa aagctattat
    11281 ccaagaatct atagactata tcagagaaaa tgaagaaata agagatgtac tagtaactgg
    11341 tggagatgct cttacattaa aagatgatta tttagagtgg attcttagcc aacttaaaga
    11401 gataccacat gttgattatg ttagattagg tactaggact cttgttacaa tgccacaaag
    11461 aattacagat gaattttgca atatgctaaa aaaatatcac cctgtatata taaatactca
    11521 ttttaatcat ccaatggaaa taactaagga atctaaagaa gcttgtgaaa agttagcaaa
    11581 tgcaggcgtt ccattaggaa atcaggcagt attattaaat ggaataaata atgataaatt
    11641 tgtaatgaga tgtttaaatc aagaattact gaaaataaga gtaaaacctt attatatatt
    11701 ccaaagtaaa catgtaaagg gaacaaaaca tttcaataca tcagtagatg atggtcttga
    11761 aatcatggag tatttaagag gatatacatc aggaatggct ataccaacat atatagtaaa
    11821 tgctccaaaa ggaggaggaa agactccttt gcttcctcaa taccttgtat caaaaggaac
    11881 agattacgtt atgcttagaa catgggaagg aaaagttata aaaatggaag atgaacctgc
    11941 tgtagatata aagaaactta taaaagaaca agcacaggat taaaaaaata tttaaagttt
    12001 ataaaaagaa acaactttaa aataaaaaga aaatggatgt caaatttata tcaatttgca
    12061 tccatttttt attagttatt tattattttg ttgttgatta actaattcaa gatatccact
    12121 tgggtggaag taatatatct cagaagtatt ttcatttaca aagtaaccaa tatcaccatc
    12181 tgcatctggt aaaaatacat aaccattaag atttttagct tttaatgctt caaaatcact
    12241 agcatttcct tgatatatgt aatcaacttc attgttgtat tttactaaaa gttctttagc
    12301 ttgttcttga gtcaatttag tattttggct tacaggaaca ttcttttgag aatctgcata
    12361 agaagattca tttactggtg cagttaataa caaacaagat aaaactaata aagatataag
    12421 tgcttttttc atactaaaac cctctttcat gtttaattat ataagattca ttacttaata
    12481 atattgtatg tttcctataa ttaaatatta ccattaaaga gaataatatt acagttacac
    12541 agctgtaact tttgattaca aaatcgtaat taaattgtat ttttattcca tttgtatttt
    12601 atcatgtcta cgatttacaa actcacagaa gtatatcttt ataacgccca tcacaggagc
    12661 agatagaatc ataccaggta taccaaagaa tcctccacca acagtaactg ctaggattgt
    12721 aaagaatggg ctaagaccta gttgtccacc tacgattttt ggctctataa atgtaacttc
    12781 aacttgttga attattatta agtaaattaa agaagttaaa gcaattgttg gattggagaa
    12841 taaattaatt attacaactg gtgccatacc tattactgga ccaaaatatg gaaccatatt
    12901 catgacaccc ataagtgtac caaataatag tgcatattca gattttaaaa agtaaagacc
    12961 aattccagaa agtactccaa ctataaaaga atctaaaatc ttacctgtaa agtatttacc
    13021 aatatttaaa tttaagcttt gacatagttc tattataaag tctccatatt ttttacctaa
    13081 ggaaatataa actacttttt ttgtaaacag taagaatttt tctttttcta ataatatata
    13141 gaaacaaatt ataaaaccta aaagaaattg tactataaat tttccaactg aaaatgtagt
    13201 gctaaatatc ccaccaagtg aaccaataag caagttacta aatttaggca ttacactcat
    13261 aattttgtca ccatactctt ttaatgtatt agggtcaact gattgtaaac tcttgcctaa
    13321 gtcgaataaa aactgctctg ttttatctat gtatgtagga atttgagcaa ctaaatcagc
    13381 taaactattt actatggatg gaactgtaaa taatataaac gaaattagta taactatgag
    13441 tacaccataa gtaagaagta acgaatatat tctcttaaaa tttaatttac tttctaagaa
    13501 ctttactagt gggttgaata tatatgctaa tacaaatgct attataaatg gtgttagtag
    13561 tgacattaat aaacttatca ctccaaaaaa atatttgtaa ttatcaataa ctttaatcaa
    13621 gatatatgaa acaactacag tgagtacaat atttagataa atttttttat cttgcaaaat
    13681 aacacaacct ttcttatatt tattaaattt agatatactt aaatctaagt taaagttaat
    13741 atttatagct tattatac
//
LOCUS       DAMKMI010000110        13752 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMKMI010000110 DAMKMI010000000
VERSION     DAMKMI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.114"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     repeat_region   650..1073
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=650..678
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaat"
     gene            1237..1410
                     /locus_tag="PC429_002799"
     CDS             1237..1410
                     /locus_tag="PC429_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDF3848290.1"
                     /translation="MSSKMGRPKVDNPKNIDVKVRFDENTHKKLLDYCEKENLTRTEA
                     IRKGVDLLLEEDK"
     gene            1534..2361
                     /locus_tag="PC429_002800"
     CDS             1534..2361
                     /locus_tag="PC429_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
                     /protein_id="HDF3848291.1"
                     /translation="MNNELMNFENNELGIKIRTIKYEDGSIGINAEDTAVGFGWCQTQ
                     NKNGKQYVSIRWETINKYCKEFGFLNLLGKDEYIPESLFYLLGMKAKNEIAVKFQTWL
                     AVDVLPSIRQTGAYITNNANPEKLREKASEIEKLQLAYNSTSMLKELLDGAGFDNKSK
                     LLTAKTLYKKAGIDLPIEIEEEESFFDTKQIASKLKIYSKSNKPAQLAVCEIIKKIDL
                     EENEVKGVWETNGSWTGTVNKYTKSVIDKVRNWIEENNRPAKIAGEKKNYHVVYKIE"
     gene            2424..2600
                     /locus_tag="PC429_002801"
     CDS             2424..2600
                     /locus_tag="PC429_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848292.1"
                     /translation="MCENLLNEYNLKTDEDVEYFVKFATLLYKLKQDNEEKFQEYAEI
                     LRGILREQQERENK"
     gene            complement(3013..3216)
                     /locus_tag="PC429_002802"
     CDS             complement(3013..3216)
                     /locus_tag="PC429_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848293.1"
                     /translation="MYFNIDKLLESKGRTRYWLAKEVGIAYPNMMKLANNETSSIKLD
                     LFEKLCLVLECTPNELVTLSSKE"
     gene            3460..3846
                     /locus_tag="PC429_002803"
                     /pseudo
     CDS             3460..3846
                     /locus_tag="PC429_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            3910..4263
                     /locus_tag="PC429_002804"
     CDS             3910..4263
                     /locus_tag="PC429_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848294.1"
                     /translation="MKLLVTKTFFERAGIELPLEVEEKEHFYDTKQIAKKLGLYTMSN
                     KPAFMAVKQIIRKLDIKEDEMKTVLETNGNWTGTVTKYSNKVTEKVKVWLKENNNPIS
                     IKGDKKNYYILYKNN"
     repeat_region   4695..5646
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4695..4723
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
     gene            5776..6291
                     /locus_tag="PC429_002805"
     CDS             5776..6291
                     /locus_tag="PC429_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3848295.1"
                     /translation="MFCSNCGYEITGAGKFCSNCGTATLADKVNNDDLFINVHGKELN
                     LTNIYKETKGDKILAIDIAMKLLGLDIKECKNIIYPAFKELSEKINIEEEKEILREDE
                     YKQTNVLEDDVARCPRCGSVSLSAHKKGFGIGKAVAGATIAGGIGLVAGNLGAKKVRV
                     TCLSCGKQFWA"
     gene            6356..8749
                     /locus_tag="PC429_002806"
     CDS             6356..8749
                     /locus_tag="PC429_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF3848296.1"
                     /translation="MATIQTSIKIFDGMTPAFRNMTNSINTTINSLERLQGRLNNPLN
                     AGNIQASQQSLNNIESILTRIEQKIGRNTNEQENFNNKIRQGSEAGSLLVSKLKSIAG
                     IYIGIKGIDSITKVADTIASTKARLNLMNDGLQTTDQLNKMIYLSAQSARASYADTAA
                     QIAKLGILAGDAFGSSAEVIKFTELMNKAFVIGGTSANEASAAMYQLTQAMGAGKLQG
                     DEFRSIMENAPLLATKIADAMGKTKDQLKELSSSGAITADVIRNALFKASDEIEKKFA
                     SMAITFSQALTMMKNDAYMIFSETLSKISGALQSVRFSEIVVSMRNVMIAISSNIYDT
                     LNIIKNILNSDFFSDFVSNVTLGAILIINGLGWVTNATLNVANVFAQNWSIIAPIIYG
                     VIAAIAIYKGVLLASTIATTVASFVNSLYAVAAYKSCAALAAQEFAIFGKISAQTMEA
                     LVTAQATAAQWGFNAALLSCPIFWIIAGIIAFVVVVFVAVAAVNKFSGTSLTVLGAIV
                     GAVFAAVAFIQNIMIWLFNRCVDVNEGIANGWNQCVYLMKQAIAKGVIFIIEKMASLN
                     DAVNNAGNALGKAFIDGANIAIRGVNKLIDLINKIPGINIGKVGEATFTPVKADNSYI
                     KQQIDSLNKWVGDAPEKIKLDRMGYKDIGAEFQKGNALGTKWQNAITDKFKDTFDINK
                     MLEDAKKKLGLDDLWNKQNPLNNLGGFGGDLGKNVKDTAGNTAKMAKTMDKSQEDLKY
                     LRDIAEQETINRFTGVNIKIDMNNTNNISKDTDVDGIVNVLTEKLNDAMIVSAEGIV"
     gene            8765..9454
                     /locus_tag="PC429_002807"
     CDS             8765..9454
                     /locus_tag="PC429_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF3848297.1"
                     /translation="MAYDFYLDGVQLPIPPPKLEIKVTNKNKIVDLINTGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGAFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDAENGSDTKVAIKLKQYRDYSTKKLVLAPPKNETGRPNVKIEPK
                     RVDSVNATNTKTYTVKAGDSLWSICQKQLGNGSLYKKVYELNKTMMDKANKGKKVPKY
                     TIYKGQVLRLV"
     gene            9447..11339
                     /locus_tag="PC429_002808"
     CDS             9447..11339
                     /locus_tag="PC429_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF3848298.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPAVIDGITWEIERRGSPSK
                     LEFTIAMDDILEFCEGNSVRIYYKGIGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFNLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQMFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLNLGDIKLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKDEKKNGPAQSITKKNTGKKVPAIFTAYYPGNNAMEGGKTDCNGKPL
                     DVKSRTVAGPMNREGVKKTWYTDDFLKKHPVFEYGDKVKIILPGTAYDNKVYTVKDNG
                     GRIYVETNGTYHIDVLLANASECKKFGRKNGYIIIGGDEEQTYQVEGNNQSSTNNNSK
                     EDKLISIAKSKLGCNYVYGAEGPNNFDCSGFTQWCYKQIGIKIPRTASAQSKAGKAVD
                     LKDRSKWKAGDLLCRIGGGSSNHVVMYIGNNQIIHSPQTGDVVKIESVNSYRKGKAYT
                     HVRRFI"
     gene            11353..11613
                     /locus_tag="PC429_002809"
     CDS             11353..11613
                     /locus_tag="PC429_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF3848299.1"
                     /translation="MSQDLLQIIKKAAMDAVETSNPMRVVFGTIESISPLRVKIEQKL
                     SIGEIFLIQTDTFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            11618..12037
                     /locus_tag="PC429_002810"
     CDS             11618..12037
                     /locus_tag="PC429_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF3848300.1"
                     /translation="MLPSDNLDYDIEDVSIINFDVRQEPSKTFKLHIEKSKIDGICDD
                     VEALKQTIFLILNTERYQHLIYSWNYGVELNDLIGEPISFVIPELERRIKEALIQDDR
                     VENVDNFEFENIKGKVQCKFSVHTKYGNIKAEKVVSV"
     gene            12038..13087
                     /locus_tag="PC429_002811"
     CDS             12038..13087
                     /locus_tag="PC429_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF3848301.1"
                     /translation="MFELMTFENIIKRMLDSVPNTLDKREGSIIYNALAPVAVELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLPATNTIAKGVFNIDIPLDSRFNLGE
                     YNYIAIERISEKTYKMKCETAGPIFELGKLIPIEYIDGLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNIQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDITNTMINDVKSNL
                     TIDSNSIVVRGEVVG"
     gene            13080..13697
                     /locus_tag="PC429_002812"
     CDS             13080..13697
                     /locus_tag="PC429_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF3848302.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVENKEIKTLHEKLNDLSS
                     DQFLEDLTPSGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSSIKPCNMTLDYTRVLVSKDNY
                     MNFGITTLIGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
BASE COUNT         5416 a         1485 c         2560 g         4291 t
ORIGIN      
        1 ttatataaaa taaaaaataa aaatttttat taaaaaattg aaaacttgat tataaagcaa
       61 ttaatttata aaatatatat aaataagtag gtatttattt acttgaattt cattgtttat
      121 ataaaaaaaa tggaaaaata tgtaataatt atatgttata ataattgtag caaggataat
      181 aatcgaaagt gcgaagggtg attattttca tattaaacgc caaattccaa ataaggaagg
      241 aggtgaaatt atatgatagg ttttttatta agcatactag ctggtgttat atcagcttat
      301 atttatgaca aaataaaaaa tcacccagac gccaataagg gtgatttaaa aaaataattc
      361 tttaaatcaa ttttgatgga aatagctact cttgtataaa gtaaattatt tccttgcttt
      421 tattatacca caaattggta cagatattca aaaataatat ttttatgata taataaaaat
      481 gtagagattt tgcagtgagc aatatttgcg ataaattgaa gtttaacaat tggaatacaa
      541 ggtattgagg gtgtgtgata aatgttatca attgcactac tcatggttca ctgcaaattt
      601 gagagagatg cgtatgtgta ggtattggaa atgccaagtt tattttgggg ttttagatta
      661 actatatgga atgtaaatta acctttttat tgttgttgtt attcttattg ttattcgttt
      721 tagattaact atatggaatg taaatgttgt aagaagtatc attctatttt ttaatctttc
      781 tgttttagat taactatatg gaatgtaaat agtacatata atgagtcttt aacatcagtt
      841 atgaaaggtt ttagattaac tatatggaat gtaaatgatt gtactttagc gtctgcacta
      901 gctttgtcta tcgttttaga ttaactatat ggaatgtaaa ttattttaca gatgaacaat
      961 tacagttact tcttgaatgt tttatattaa ctaagtggta tgtaaataat ggaagcaaga
     1021 taatttttaa aggtatggat aattgtttta tattaactat gtggattcaa aattaaatag
     1081 aaaaaagaaa gcacttactt aaatagtagg tgcttttgtt ttgctcaaaa ttggtcggtt
     1141 gagtaaaata attagaaaaa attgggataa gttattgact ttttggaaca caaataatat
     1201 aattatatta tggaacacaa aaagtgaggt gaaacaatga gttctaaaat gggaagacca
     1261 aaagttgata atcctaaaaa tattgatgtc aaagtaagat ttgatgaaaa tactcataaa
     1321 aaattattag attattgtga gaaagaaaat cttactagaa cagaagcaat aagaaaagga
     1381 gtagacttac ttttggagga agataagtaa aaaagtagcc caacgccgac caaagcatac
     1441 tggactactt aacctagagt tatctctata tgaaatattc tatcatgtaa agataactct
     1501 ttcaagataa taaatcgaaa gggtgatttt tgtatgaata atgaactgat gaattttgaa
     1561 aataatgaat taggaataaa aataagaact attaaatatg aagatggaag tataggaatt
     1621 aatgcagagg atacagctgt agggtttgga tggtgtcaga cacagaataa aaatggtaag
     1681 caatatgtat caattagatg ggaaactatt aataaatact gtaaagaatt tggtttcctc
     1741 aacttgttgg ggaaagatga gtatatacct gaatcattat tttatctttt aggtatgaaa
     1801 gctaaaaatg aaatagcagt aaaatttcaa acatggttag cagtagatgt actaccatca
     1861 ataagacaaa ctggtgcata cataactaac aatgctaatc ccgaaaaact aagagaaaaa
     1921 gcaagtgaga ttgaaaagtt acaattagcc tataacagca catctatgtt aaaagaattg
     1981 ctagatggtg caggatttga caataaatcc aaactattaa cagctaaaac attatataaa
     2041 aaggcaggca ttgatttacc tatagaaatt gaagaagagg aatctttctt tgacacaaaa
     2101 caaatagcat ctaaactgaa aatatattct aagagtaata aaccagcaca gttggctgtt
     2161 tgtgaaatta ttaaaaagat tgatttagaa gaaaacgaag tcaaaggcgt ttgggaaact
     2221 aatggttctt ggactggtac tgtaaataaa tatacaaaga gtgtaataga taaggttaga
     2281 aattggatag aggaaaataa tagacctgct aagattgcag gtgaaaagaa gaattatcat
     2341 gtggtttata agattgagta aatttatcag ttgtattaaa taatttagtt tagtttattt
     2401 tagttttgag ggggattaat acaatgtgtg agaatttact taatgaatat aatttaaaaa
     2461 ctgatgaaga tgtagaatac tttgtaaagt ttgctacatt attatataaa ttaaaacagg
     2521 acaatgaaga aaaatttcaa gagtatgcag agatattgag aggtattctt agggaacaac
     2581 aagagagaga aaataagtaa aataatatag ataaagcact tgaatattac atagtttcaa
     2641 gtgctttgtt tggtataaaa tggtataata gagataagag ttatattaac tatgtggtat
     2701 gtaaagaatg tataaggcat cttctcaaat tctgcgttgg tcattttata ttaactatgt
     2761 ggtatgtaaa gtgcgttaac tgcaataatg ctgaactcat ttcttgattt tatattaact
     2821 aagtggtatg taaatgttaa ttccttcaac cttctaatag aagatgcttt taggtttata
     2881 ttaactaagt ggtatgtaaa tgtttcaaca tttgacatgt gctgtggagt aatgtttatg
     2941 ttttatatta actatgtgga ttcaaaatta aatagaaaaa agaagcactt acttaaatag
     3001 taggtgcttt tgttattctt tgctagataa agtaacaagt tcattaggtg tacattcaag
     3061 aacaagacaa agcttttcga ataaatctaa ttttatagaa cttgtttcat tgttagctaa
     3121 tttcatcatg ttaggataag ctatacctac ttctttagct aaccaatatc tagttcgacc
     3181 tttggattca agtaatttat caatgttaaa atacattttt tcacctctta agtatattat
     3241 acaacaatta tataatgttt gcaatatata atatgcaaac aatataatta tataatgttg
     3301 acattatata atgtgtgcga tataatatac ttaaggaata aaaaataaga gccactcgcc
     3361 cctaccaagt ttgagtaact cttattgaca tatactacat atacattaat tatagtatat
     3421 gtcattcctt aaaaaaaatc aattaaggag tgtgttttta tgaacaattt agtactaatt
     3481 aacaatcaag aagtgcaagt taaggaattt aataatcaaa gagtagttac atttaaagaa
     3541 attgacagag tacatgaaag agtagaagga acagcaggta gaaattttag agaaaataag
     3601 aagcacttta taaaaaatga ggactatttt tatttagaag gaaaagaatt atcaacaatt
     3661 aaacagacaa cgaatttcgt tggcagtaat gcaagagaat taatcttatt aactgaaaca
     3721 ggttatctaa tgttagtaaa atcttttaca gatgatttag cttggaaagt acaaagacaa
     3781 ttggtaaata gttattttag agttaaagaa gaaaagaaag aaatgaaagc attagaaaaa
     3841 ttagaaacag ttaatgagtc aattaggtta ataactccaa tttttgatga tttaaacatt
     3901 gataaaagta tgaaacttct tgtaactaag actttttttg aaagagcagg tattgaattg
     3961 cctcttgaag ttgaagaaaa agaacatttt tatgatacaa agcagattgc aaaaaaatta
     4021 ggactttaca caatgtctaa taaaccagca tttatggcag ttaagcaaat tataagaaag
     4081 cttgatatta aagaagatga aatgaaaaca gtattagaaa cgaatggcaa ttggactggt
     4141 actgtaacaa aatattctaa taaggttaca gaaaaggtca aagtgtggtt gaaagaaaat
     4201 aataatccta tctcaatcaa aggagacaag aaaaactact atatattgta taaaaataat
     4261 tagattttag tttttggggg ggtaatacaa tgtatgagaa tttacttgat agtatagata
     4321 ttgaaaagag aaaagaagaa tttagaatta aacttttgaa aataagagaa acagatatag
     4381 atatatatat ataataagat agaaagaata gtatataaac tttctgagaa aaaattagag
     4441 aaaaataatt aaataaatag ataaagcact tggatattat gttgtttcaa gtgctttatt
     4501 tggtataaaa tggtataata aaaatatagg aattttgcag tgttcgattt ttctacttaa
     4561 gtatagttta acaattggaa tacaaggcat tgagaaggtt tgataagtgt tatcaattgc
     4621 actattaccc gctcactgca aatttgagaa agttatatgt gtgtaagtct tggaaataca
     4681 caatttattt tggggtttta tattaactat gtggtatgta aatcttttta aaaaagcacg
     4741 tcaattacaa tactaaaatt gttttatatt aactatgtgg tatgtaaatt tgaaaactgc
     4801 atgttcttta acaaaagcat ctaattgttt tatattaact atgtggtatg taaatgtatt
     4861 actcccaatt atgaacatag gatatagtct ttgttttata ttaactatgt ggtatgtaaa
     4921 ttatagtttg ccatatgttc ctggttcgta tgattggggt tttatattaa ctatgtggta
     4981 tgtaaataaa tttaatatta ctggaaaagg agaaatggat atcggtttta tattaactat
     5041 gtggtatgta aattttgtta taagaatatc gccaccttcc ttttctggtg ttttatatta
     5101 actatgtggt atgtaaattt ttctgcatct tctactatgt tatattcttc taatggtttt
     5161 atattaacta tgtggtatgt aaatgcacat ttaacttcaa gtcttagagt attatctgca
     5221 agttttatat taactatgtg gtatgtaaat ttcgttcaac ttttatctta gcttctcctg
     5281 ctacttgttt tatattaact atgtggtatg taaatgtact agaaaatgca ctacctgcat
     5341 tcattcctat acggttttat attaactatg tggtatgtaa atgtgctatt tccaactttt
     5401 ttttcaagtt ctgaatactg ttttatatta actatgtggt atgtaaatta gttagtccca
     5461 tatcgttatg gtactgcatt aacgcgtttt atattaacta agtggtatgt aaatagtgta
     5521 aaaggcattt tttcaaattg tgcattggtt tggtttatat taactaagtg gtatgtaaag
     5581 gctaatgttt gagtatttaa aagcatctga gcaaacaatt ttatattaac tatgtggact
     5641 taaaattaaa aataattcaa aaacacttac aaatgagtaa gtattttttt aatgaaagga
     5701 ggtgataata atgtaaaaat tttacgtata tagtataata gtgctataaa ataagtatta
     5761 tgtgaggtga ttgttgtgtt ttgttcaaat tgtggttatg agataactgg tgcaggcaaa
     5821 ttttgctcaa attgcggaac agctacatta gcagataaag ttaacaatga tgatttattt
     5881 ataaatgttc atggaaaaga attaaatctg actaatattt ataaagaaac taaaggagat
     5941 aaaatcttag caattgatat tgcaatgaag ttactagggc tggacataaa agagtgtaaa
     6001 aatattatat atccagcttt taaagaatta agtgaaaaaa taaatatcga agaggaaaaa
     6061 gagatattga gggaagatga gtataaacaa actaatgtac ttgaagatga tgttgctcgt
     6121 tgccctaggt gtggctctgt ttcattgtct gctcataaga aaggttttgg cataggaaaa
     6181 gctgtagcag gggctactat agcaggaggc attggtttag tagctggaaa tttaggagca
     6241 aagaaagtta gagttacatg tttgagctgt ggtaaacagt tttgggcata aataataaac
     6301 acttactaat gtaggtgttt ttttatatgg aaatttatga aaggagagtg aggaaatggc
     6361 tacaatacaa acttcaataa agattttcga cggaatgaca ccagcatttc gtaatatgac
     6421 taattctatt aatacaacaa ttaatagttt agagagattg caaggcagat tgaataatcc
     6481 actcaatgca ggtaatatac aagcttctca acaaagtttg aataacatag aaagtattct
     6541 cacaaggata gaacagaaaa ttggaagaaa tacaaatgaa caggaaaact ttaataataa
     6601 aataagacaa ggtagtgaag caggttctct attagtgtct aaattaaaaa gtattgctgg
     6661 gatatacatt ggaataaaag gaatagatag tattacaaaa gtagcagata caattgcaag
     6721 tacaaaagca cgtttaaatc taatgaatga tggcttacag acaacagacc agcttaataa
     6781 aatgatttat ttgtcagccc aaagtgcaag agctagttat gcagatacag cagcacagat
     6841 tgctaaactt ggaatacttg caggagatgc ttttggaagt tcagcagagg tgataaagtt
     6901 tacagaactt atgaataaag cttttgtaat tggaggaaca tcagcaaatg aagctagtgc
     6961 agcaatgtat caattaacac aagccatggg tgcaggaaaa cttcaaggtg atgagttccg
     7021 ttccataatg gaaaatgcac ctttattagc cactaaaata gctgatgcaa tgggaaaaac
     7081 taaagaccaa ttgaaggaat tatcaagtag cggagcaata acagcagatg ttataagaaa
     7141 tgcactgttt aaagcttctg atgagataga aaagaaattt gcaagtatgg caatcacttt
     7201 ttctcaagct ctcacaatga tgaaaaatga tgcttatatg atattttctg agactctcag
     7261 taagataagt ggagctttgc aaagtgtacg ttttagtgag attgttgtat ctatgcggaa
     7321 tgttatgatt gcaatatctt caaacattta tgatacatta aatattataa aaaatatatt
     7381 aaatagtgat tttttttcag attttgttag caatgttaca ttaggagcta tactaattat
     7441 caatggactg ggatgggtta ctaatgcaac actaaatgtt gctaatgtct ttgctcaaaa
     7501 ttggtcgatt attgctccta ttatttatgg agtgatagca gcaattgcga tatataaagg
     7561 agtattactt gcaagtacaa ttgccactac ggtagcatct tttgtaaatt cattgtatgc
     7621 agtagcagcg tataaatctt gtgcagcttt agcagcacaa gaatttgcga tatttggaaa
     7681 aatatctgca caaacgatgg aagctttggt aacagcacaa gcaacagcag cacaatgggg
     7741 ttttaatgca gcattattat cctgtccaat tttttggata atagcaggta ttatagcatt
     7801 tgtagtagta gtttttgttg cagtagcagc agtaaacaaa ttctcaggaa caagtctgac
     7861 tgtactagga gcaattgtag gtgcagtatt tgcagcagta gcatttatac aaaacataat
     7921 gatatggcta tttaatagat gcgtagatgt aaatgaaggg attgcaaatg gatggaatca
     7981 atgtgtatat ctaatgaaac aagctattgc aaaaggtgta atctttataa ttgagaaaat
     8041 ggcatcatta aatgatgctg taaataatgc tggaaatgca cttgggaaag ctttcataga
     8101 tggggcaaat atcgcaatac gaggtgtaaa caaattaatt gacctaataa ataaaatacc
     8161 agggataaat attggtaaag ttggagaagc aacatttacg cctgtcaagg cagataatag
     8221 ttacatcaaa caacagattg acagtttaaa taaatgggta ggagatgcac cagaaaaaat
     8281 aaaattggat agaatgggat ataaagatat tggagcagaa tttcaaaaag gaaatgcact
     8341 tggaactaaa tggcaaaatg ctataactga taaatttaaa gatacttttg acattaataa
     8401 gatgctagaa gatgcaaaga aaaaattagg attagacgat ttgtggaata aacaaaatcc
     8461 tttaaacaac cttggtggat ttggtggaga tttaggaaaa aatgtaaaag acacagcagg
     8521 aaacacagcc aaaatggcta aaacaatgga taaaagtcaa gaagacctta aatacttaag
     8581 agacattgca gaacaagaaa caataaacag attcacagga gtaaacataa aaattgatat
     8641 gaacaatact aacaatataa gtaaagacac ggatgtggat ggtatagtaa atgttttaac
     8701 agaaaaactg aatgatgcta tgattgtatc agcagaagga atagtttaga aaggagggat
     8761 ataaatggct tatgactttt atttagatgg agtacaatta ccaatacctc cgccaaagtt
     8821 agagattaaa gttacaaata aaaacaagat agttgatttg ataaatactg gagaagtaaa
     8881 catactaaaa aaagaaggat tatctgaaat aagttttgaa gcagaattta cacataataa
     8941 gttgccattc tatcgtggag cttttaggga tgttcaattc tttttaagta aactggaact
     9001 attaaaaact gattgtaagc catttcaatt tattgtatcg agggaattag gtaataaagt
     9061 actatttaac actaatataa aagtatctct tgaagagtat gctatttcag aagatgcaga
     9121 aaatggttca gatacaaaag ttgcaataaa gttaaagcaa tatagagatt actcaactaa
     9181 aaagttagtt cttgcccctc ctaaaaatga gactggtaga cctaatgtaa agatagagcc
     9241 aaaacgagtt gattcagtca atgccacaaa cactaaaaca tatacagtaa aagcagggga
     9301 tagcctttgg tcaatttgtc agaagcaact tggtaatggt tcgttataca agaaagtata
     9361 tgagttaaat aaaacaatga tggataaagc taacaagggt aaaaaagtac ctaaatatac
     9421 catctataaa gggcaggtgt tgaggcttgt ctgatgattt agttctggcg aatgatagag
     9481 atataaggct agttattgca cattgggaag atttctacga acctgctgtc attgatggta
     9541 tcacatggga gatagaaaga agaggttcac cttcaaagtt agagtttact atagcaatgg
     9601 atgatatatt agagttttgc gaaggtaatt ctgtaaggat atattataag ggaattggca
     9661 tattttatgg atatatattt cagaagaaaa gagataaaga aaatcacatt aaaatagttg
     9721 cttatgacca gttgagatat tttaaaaata aagatactta tgtatatagt aataaaacag
     9781 caagtgaact tgtaaagatg ttggctaaag attttaattt aaaatacaat gtcatagaag
     9841 atacaaaata taaaatatcg agagttgaag aaaataaaac actctttgat atggtcttaa
     9901 ctgcactaga tgatactcta agagagaaaa aagaaatgta tgtgttatat gatgattttg
     9961 gaagaataac attaaagaat gttgcatcaa tgaaattaga tactgttatg aacaatgatg
    10021 taatagaaga ctttgactat aattcatcaa tagatagtga tacttacaca aagattaaac
    10081 ttgtgagaga taatgaagaa acaggaaaaa gagatgtgta tattgctcaa gactcaacgc
    10141 atatgaggag ttggggaata cttcaaatgt ttgatacagt agacaaaaac atgagtgaag
    10201 cagagataaa acaaaagtgt gatatacttc taaaactata taataagaaa actaagtcat
    10261 taagtttaaa aaatgtactt ggagatatta gagtgagagc aggttgttta gtacctgttt
    10321 ttttaaatct aggagatatt aaacttcaaa attatatgct agttgagaaa gtaaaacata
    10381 cttttgaaaa taacagtcat tttatggatt tgactcttgt tgatggagat gaatttgctt
    10441 cttattcttc aagctcatat tcaagtggaa atactaacaa taaggatgaa aagaaaaatg
    10501 gtcctgcaca aagtattacg aaaaaaaata caggtaaaaa agttcctgct atatttactg
    10561 catattatcc agggaacaat gcaatggaag gcggaaaaac agattgcaat ggaaagccac
    10621 ttgatgtaaa atcaagaact gttgctggtc caatgaatcg agaaggagtt aagaaaactt
    10681 ggtatactga tgattttcta aagaaacatc cagtttttga atatggagat aaagtaaaaa
    10741 ttatacttcc tggtactgcc tatgacaaca aagtatatac agttaaagat aatggaggaa
    10801 gaatatatgt tgaaacaaac ggaacatatc atatagatgt actattagct aatgctagtg
    10861 aatgtaaaaa atttggtaga aagaatggct atataattat aggtggagat gaagaacaaa
    10921 catatcaagt tgaaggtaat aaccaaagta gtacaaataa taactctaaa gaagataaat
    10981 taattagtat agcaaaaagt aaactgggtt gtaattatgt gtatggagca gaaggtccta
    11041 ataattttga ttgcagtggg tttactcaat ggtgttataa acaaataggt ataaaaattc
    11101 ctcgtactgc ttctgcacaa agtaaagcag gaaaagcagt agatttaaaa gatagaagca
    11161 agtggaaagc aggagactta ttatgtagaa ttggaggagg aagtagtaat catgttgtaa
    11221 tgtatattgg aaacaatcaa ataattcatt caccacaaac aggagatgtg gtaaaaatag
    11281 agtctgttaa ttcttataga aaaggaaaag catacacaca tgtgagaaga tttatataag
    11341 tgaggtggca atatgagcca agatttatta cagataataa aaaaagctgc aatggatgca
    11401 gtagaaacaa gcaacccaat gagggttgta tttggaacaa tagaaagtat tagtcctcta
    11461 agagttaaga tagaacaaaa actatctatt ggtgaaattt ttctaataca aacagataca
    11521 tttaaaagat atacagataa aaaaatagga gataaattag tcttaattcg tatgcaagga
    11581 gggcaacaat acttgatttt agataggatg tgataaagtg ttaccaagtg ataatttaga
    11641 ttatgatatt gaagatgtat caataattaa ttttgatgta agacaagagc caagtaagac
    11701 atttaaactt catatagaaa agtctaagat tgatggtatt tgtgatgatg tagaagcatt
    11761 aaaacagacc atctttttga ttttaaacac agagagatac caacatctaa tatatagttg
    11821 gaattatgga gtcgagttga acgaccttat tggagagcct atatcctttg taataccaga
    11881 gttagagaga agaatcaaag aagcactaat tcaagatgat agggttgaaa atgtagataa
    11941 ttttgagttt gaaaatataa agggtaaagt acaatgtaaa ttttcagttc atacaaaata
    12001 tggaaatata aaagcagaga aggtggtgag tgtataattg tttgagttaa tgacatttga
    12061 aaatataatt aaaagaatgt tagatagtgt acctaacaca cttgataaga gagaaggttc
    12121 tataatatac aatgccttgg caccagttgc agtggagctt acagaaacat atattgctat
    12181 ggatgaatta ctagaccaaa cattcgtaga tactgctagt tattactatt tagagaagag
    12241 atgtaaagag cgaggaatta cacctttacc agccactaat acaattgcta aaggagtttt
    12301 taacatagat attccacttg attctaggtt taatctagga gaatacaatt atattgcaat
    12361 tgagagaata tctgaaaaaa catataaaat gaaatgtgaa actgctggac ctatatttga
    12421 gttaggaaaa ctaataccta ttgaatatat agatggtctt gaaactgctg aactaactga
    12481 aatcttgata aatggagagg atgaagagtc agaagatagt ttaagacaaa gatattatga
    12541 tagcctaaat tcacagagct ttggtgggaa tatacaaaac tataaagatg aagttaacaa
    12601 aatacaagat gttggaggag ttaaggttta tcctgtgtgg gacggtggag gaactgttaa
    12661 gttagtaata attaactcta atttcaaagt accatcagag gatttagtta atttagtgca
    12721 agaagaaatt gacccaattg gacatcaagg acaaggctta ggattagcac caatagggca
    12781 taaagttact gttacaggtg ttgtaagtac aactataaat atatcagcag agataacata
    12841 caaaaatggc tacacttggg agaatataaa atcaattgca gaagaagcaa tagacgacta
    12901 tttaaatgaa cttaacatga gttgggaaga tgaagaaaac ttaatagtcc gtatatctca
    12961 aattgaaact agattactta gtatagatgg agtgttagat attacaaaca caatgataaa
    13021 tgatgttaaa tctaatctaa caatagatag taacagtata gtagtgagag gtgaggtagt
    13081 tggataaaga gattaatcta ataaattact taccacaaat tctacaagat aaagaagaat
    13141 atataaaagt atttaatgta gaaaacaaag aaataaaaac actacatgaa aaattaaatg
    13201 acctatcaag tgaccagttt ttagaggatt taactccaag tggtataaaa agatgggaaa
    13261 agataatgtc tataactcct aaaagtaatg agagtttaga agatagaagg tttaggattt
    13321 ttagtaaata tataagtaaa ctaccttact cagagagatt tttaaggaac tggctagata
    13381 gtatagttgg agaaggcaat tatgaattaa ctattaataa tgctacttat aatatacatc
    13441 ttgaaagtga tgctagaaat caagattggt ttgaggaggt tcattctttt gtaagtagta
    13501 ttaaaccttg caacatgact ttagattaca ctagagtgct tgtaagtaaa gacaattata
    13561 tgaattttgg tataacaacc ctaataggtc aagaaataac tatataccct tggagtccac
    13621 cagatataga aacttatgga gaaattgatg tattaactgg caatggagtt ggataccaag
    13681 agataacaat attttaggag gtgatatatt ggctatagat aaaagttatt acactataat
    13741 tacagatgta gg
//
LOCUS       DAMKMI010000111        13271 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMKMI010000111 DAMKMI010000000
VERSION     DAMKMI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.115"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            133..1992
                     /locus_tag="PC429_002813"
     CDS             133..1992
                     /locus_tag="PC429_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3848303.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2284..2883)
                     /locus_tag="PC429_002814"
     CDS             complement(2284..2883)
                     /locus_tag="PC429_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3848304.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(2986..3783)
                     /locus_tag="PC429_002815"
     CDS             complement(2986..3783)
                     /locus_tag="PC429_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF3848305.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3896..4001)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4093..5079)
                     /locus_tag="PC429_002816"
     CDS             complement(4093..5079)
                     /locus_tag="PC429_002816"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF3848306.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5563..7293
                     /locus_tag="PC429_002817"
     CDS             5563..7293
                     /locus_tag="PC429_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF3848307.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7403..7483
                     /locus_tag="PC429_002818"
     tRNA            7403..7483
                     /locus_tag="PC429_002818"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7437..7439,aa:Leu,seq:tag)
     gene            7818..8333
                     /locus_tag="PC429_002819"
     CDS             7818..8333
                     /locus_tag="PC429_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF3848308.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8825..8929
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9139..11613
                     /gene="gcvPA"
                     /locus_tag="PC429_002820"
     CDS             9139..11613
                     /gene="gcvPA"
                     /locus_tag="PC429_002820"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF3848309.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11613..13070
                     /gene="gcvPB"
                     /locus_tag="PC429_002821"
     CDS             11613..13070
                     /gene="gcvPB"
                     /locus_tag="PC429_002821"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF3848310.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4975 a         1572 c         2191 g         4533 t
ORIGIN      
        1 aactatgtaa aacaaggttt atatattcta attgtataga aaatttattg taaacgcatg
       61 tatattatta gaaaaaatag tttgaaggta ttcaataaac ataaaaaaca atatagggga
      121 taaaggtgtt ttatgaagag atttttgagg agaattatct tagttttatt tattttgttg
      181 atttttataa gttttatttc tattaaatta attcataatg ttggagatta tggaaaattg
      241 attaattatg ttgggattgt tcgtggagct agccaacgtc taactaaact agagatgaat
      301 catcaaccaa atgatgaatt aatagaatat attgatgaaa tcctacagga gttgattaca
      361 gggcatggag attatggttt agtaatcact gattgtaatg aatacaatga ggacttactt
      421 ttattagaaa aaaagtggga agatttaaat attgaaatta aaaaagtaag aatgaaagaa
      481 cagaataacc agttattaag tataagtgaa gagttttttt ctcttgcaaa tgatacagtg
      541 tttaaaatag aaaacttttc taaagaaaaa agtaattact taatgacttt aataataatt
      601 atatctatta taggtatatt agcatgtatc atattgattt tacaatatag taaaaaaatg
      661 gttagactgg aaaaattaaa tgtagattta aaaaatattg catataaaga tgaactaaca
      721 ggagtaaata ctatagaaaa atttaaattg gatgcaaatc aaaatatttg tatacataag
      781 gataagaaat ttgctgtatt ctatattgat tttgaaaact ttaaatacat taatgatata
      841 tttggatatg attatggtga catgatactg aaaagatatg ctaatcttat gatgaatgac
      901 atagggaaat atgaaatatt tgcaagagaa attgcagata gatttgttgc gttaagatgc
      961 tatgtagata aggaagattt agtggtaaga caacgagatg tagacaatga actaattaat
     1021 ataacaaatg aaattaaaaa taaacatagt attactgttg ttagtggaat ttgttgtatt
     1081 gaagatgtaa atgaaaaatt aagtatagat ggtttaataa atagagcaaa ttttgcacaa
     1141 aaaactgtta agaataaacc aggtactaat tatgcttttt ataatgacag tattcgtaaa
     1201 aaaatgattg aagaaaatac tatcaagagt agaatacatg aagctattga aaaaagagaa
     1261 tttatagttt atttacagcc taaagttaat ttacatagtc aaaaaataaa ttgtgcagaa
     1321 gctttagttc gttggcttac tccggataaa ggtattatat cgccagcaat atttattcca
     1381 gtacttgaga agaatttttt tattgcctta gtagataagt atgtttttga ggaggtttgt
     1441 aaatggatta gaaagaggtt agatgaaaat aaaccttttg ttcagatatc agttaatgtt
     1501 tcaagaatac aattttataa cactaaattt gtagaaactt attccaatat acaaaataaa
     1561 tacaggatac ctaaaaatac tatagaaatt gaatttacag agagtgttgc ttttgagaat
     1621 caaaaacatc ttttggaaat tatacatgat ttgcatgaaa atggatttac ttgttcttta
     1681 gatgattttg gaaaaggtta ttcgtcttta agtgttttaa aagatttacc atttgatgtt
     1741 ttaaagcttg attctatgtt tttcaaggct agtttagata aagataaaga gaaaatagta
     1801 ataaaaaata ttgtacatat gttaaaagaa cttaatatca caactgttgc tgagggcatt
     1861 gaatgcaagg aacaagttga atttcttagg gatattgggt gtgatttagt tcagggattt
     1921 gtattttata aaccaatgcc aatattagaa tttgaaaaaa tattggataa agagtttgtt
     1981 tataatagct aaaatgtact ttataaaata tgaactaatt aagtaaaatt attttgtact
     2041 gaataaattt ttactttatt tacagaataa tgattattta cagaaaagcg cgaaaagtgt
     2101 attcctttaa gtgtgggatg gatagcacaa atagaggaga aaaaatattg gccatataag
     2161 aatgcataat aatataaata tttgaaaggt cagcattata taaaaattaa aaagaaaata
     2221 aataattttt ctataaaaaa gagactatga gaaaatttac tcatagtctc ctaaataatg
     2281 tatttatttt cttcctgcaa cccattttaa tccccaatga aattcagtat ccatttctct
     2341 atgacctttg aagtatttaa ctactttttc aacactgaat gtctcatcat ttacattttc
     2401 tatcaatgct attgaacaca tattatagcc atttttatgt tcatcaagtt tgacaactaa
     2461 gtctgaatct tgtttttgtt tgattgttac aattccatca gcttcagacc agttagctac
     2521 accttcgtat ataaaagcat aaataagtat tctttttata tcttttattt tattaccatt
     2581 tattcttaaa ttttctcctt gtgtattact tcctgttctg tcatctccat ctaattgagc
     2641 aaaaggtgga ttgtctaaac taccaaaggc atttcctaat gcttgaacag ctccttttac
     2701 accatttttc atctcaaata ggcaacccaa atctaaatct atattactgc ttcttaaaga
     2761 gccaaaaaaa cccttttttt gtactttttg attccaattt aaattaacta agatttcacc
     2821 taaatcgcta ttttcctttt ttgtaaggtt tattttttgt ccctttttta attctatagc
     2881 catagtaatc ttcctccaca aacatagatt tatttaaatg gtagcactta ataagtcaat
     2941 attcaacaag attagaataa taatattaaa attatactaa atatactaaa aagtaggaat
     3001 cacaatacct ttgtattttt cttcaattaa gcttcttgct ttatctgatg taagaacatc
     3061 ttgtagaact ttaatctttt cactgtcttt atcttcagtt ctaaatgcta aaagatttgc
     3121 atactcgtca tttataggag ctgtataaat tccgtttttc ttggcatcaa gaccagcagg
     3181 tacagcataa gtagtatcta taaatgcagc agtcacatct ggaagagaag atggaatttg
     3241 agcaatatct aactctttga attgtattcc ctttggattt tctattatgt ccttaggact
     3301 aactaatcca tcccctttag gtaacgttaa aagtccaata ctttcaaggt atctaaggtt
     3361 cttagaacaa gaagatgggt tattgcttat tgcaattgta tctccttttt taaattcatc
     3421 aacagatttt atcttataag agtaaagtat tgctgggcat acatatagtt ttttaccagc
     3481 cattattttg taacctttgg ccttaacagc ttcttttaga taaggttcat gttgaaatag
     3541 atttgcatca atttcaccat tatttaaagc ttcgtttgga agtatgtaat catcaaagtt
     3601 tttaacttct aaagtatatc ctttttcttt aataagtggt tttaattctt ctaataactc
     3661 tcctcctgga actaaagtag caccaacaac aattttttta tcatctttag ctttagaaca
     3721 tccaaccaca gctaaagtta atactagaca taataacaaa cataataatt ttttgaattt
     3781 catataaatt ccctcctgaa tataattgat tagttaaaaa taaaatacca ttctttatca
     3841 cactgcaaaa taaaaataag cctgagttta aactcaggct ttaaaagcta atttagactc
     3901 atctttcagt ataatactgc aggatttagc accaatgtat ataaaaatat actggttgct
     3961 gggtttcata gggccagtcc cttcaccact ctggataaga agtatcaatt tttaattatg
     4021 ttataaatat tatcatgatt ttttacgtct gtaaacataa ttttataaat attttaaaat
     4081 ctttaatgta ttttatttaa cacctaaaat tccagaaact agaatatcag tatcagcacc
     4141 taagaaatag tcatttattt caatagaatc cagagtattt ctcaaagcat tttcttcatg
     4201 ccttatacca ataagtttat tttctacaaa tgcaacatca cattttccaa aaaaatctcc
     4261 aaaaaatttt atactagtta ttatgccttt atcaacattt aaattaaact caacatttcc
     4321 accagaatat tttaattcat ttgaaagagt atattttggt gaattaccaa aattccaatc
     4381 ccaagtttcg tattttgatt tagtaagctt ttctatttgt tttatatcat gctcactcaa
     4441 acaataatta gtattatcag tatttgtttt agaaagataa tccattatag catctttaaa
     4501 ttctaatatg tccatttttg aatgtaaatg ttcacttata ttggttacac gagattttac
     4561 agattttatt ccttttcctt caaattttat aggtttaacc tttaatgctt ttgagaggtc
     4621 atttatttca gatgaaaata aaagagtacc atgatgcatt actttgtttt tgtagttgta
     4681 ttgagcattt ccagaaaatt tcttgccatc tataagaagg tcatttcttc cagaaaattc
     4741 ggcattgaca tctagtgttt taagtaaatc tattatgggc tgagtgaatc ttctgaaatc
     4801 actaaaatta ttattgttac aagctataaa tgtaaaattt atatttccca aatcatgaaa
     4861 tacagctcca ccaccagact gtctcctaac gacaggtata gaatattctt ttatatattc
     4921 taaatttatt tcagaaagtg tgttttggtt ttttccaaca attatagaag gttcattcct
     4981 ccataaaata aataagtctt catcaaaatt ttttagaaga tattcttcca ttgctaaatt
     5041 aaaataaggg tttgtttttt cattataaat taataacatt aaagtagttc ctccatagaa
     5101 ttgataaaat ttttattatg aatacaatta attttataat tcgacaataa tataaaaact
     5161 cctttaaatt agaaatttta aaaactacct tgcaaagttt aaataaataa tatatactca
     5221 aaatatgttt aataaaagtg aatagaaggg tagaggcgca ataatctatc agtactaata
     5281 aggagttaag cacagagatt tattaggaaa ggagatattg ccgaagtttt ataatcgatg
     5341 ctttaagatt atattgctgg gggtacatag aaaatatgta caactgtcac aggtttattt
     5401 gtggtgagct atcatttaag gaattatatt atatttattt ttactattgt tgatatattg
     5461 taatgtttaa gccttgagtg tttaccactc aaggcttttt tagttttatt gtaacataat
     5521 accctgtaca gttcacaaaa ttaaaaggag agggagattt atatgaggaa caaaaaaatt
     5581 aatattattt ttctgacgac aatcatgttc ataatgtcaa cagtaatggt ttttgcagaa
     5641 gaagatatag acactattgc actagccaat gcagaaaaat ttgggatttt aactttaata
     5701 ccaccattag ttgctataat acttgcattt ataactaaaa acgtaataat ttctttactt
     5761 ataggtattc tttctggtag ttttataatt aaagctagtg gtattaatgt ttttgcgaca
     5821 tttatacagg catttttaga tttagtagac agagctttag tatctttagc agacccttgg
     5881 aatgcaggga taatactaca ggttttggca attggaggag taataaatct tgttgctaaa
     5941 atgggaggag ctaaagctat agctgaagca cttgctaaaa gagcaaagtc agcaaaaggg
     6001 acacagctta taacatggtt tttggggctt ttagtattct ttgatgatta tgccaattca
     6061 ctaatagtag gtcctatgat gagacctgtt gcagataaaa tgaaaatatc gagagaaaag
     6121 ctagcattta taatagatgc cacagcagca ccagttgctg gtttagctat aatatctaca
     6181 tggataggtc ttgaggttgg tttaatacat gatgcttttg aaagtataag tatagatgta
     6241 gatgcatttg gtatattttt aaatacaata ccatttagat tttacaatat attaatatta
     6301 gctttcattg taatatcagc attattatta aaagaatttg gtccaatgag aaaagctgag
     6361 ataaaatcta gaagtagaaa aattagtata gaccttgatg aaggcgtaga agagttggat
     6421 gatttagctc caaaaaatgg agtaaaattg agtgtatgga atgctataat cccaatagga
     6481 acattgataa tagttgcatt agcatcattt tattatagtg gatatacatc tataatggga
     6541 ggagacgata aggctctgat acaactattt acaaattctc catattcttt tgaagcaata
     6601 aaagaagcat ttagtgcatc tgatgcatct agagcattat tccagtctgc acttgttgca
     6661 agtttagttg ctattataat ggctgtagta aaaaagattt ttacaatatc agaagcaatt
     6721 gatgtttgga tagatggaat gaaaagttta gtaatcactg gtgtaatatt aatacttgca
     6781 tggtcattaa gttcagtaat aaaagagtta ggtacagcta aatttttaat acacctatta
     6841 tcaggttcac taccaccatt cttattacca agtttaatat ttggtttagg agcaataata
     6901 tcttttgcta ctggaacagc ttatggaaca atgggtatac ttatgccact tgcaattcca
     6961 ttagcatact cattaaatcc tgatatgtct tatgtaatag taagtactag tgcagtttta
     7021 acaggtgcaa tatttggaga ccattgttct ccaatttcag atacaacaat actttcttca
     7081 atgggagctg gctgtaatca tatagaccat gttaacactc aaatgccata tgctatattt
     7141 acagcagtaa tcactattgt atttgggtat ataccagctg gacttggatt gccaatatat
     7201 atagtattac cagtggcaat agctgctata tttgtaggta ttcaaataat tggtaagaaa
     7261 gttgatgaag cggaaataga acttgtagaa taaagatata aataagtttc accttttatt
     7321 atcactattt tgttagtttt tagataattt taaaaagtta ttgaaaataa tggcgaaata
     7381 tagtaaaata aaagagtgat aagcggatgt ggcggaattg gcagacgcac tagacttagg
     7441 atctagcgcc attggcgtgg gggttcgact cccttcatcc gcactttaca gtacttaatt
     7501 gtactgaaaa aaactggggt tgctggcttt cggagtgggc tatcccgttt ttattttttt
     7561 gtatatataa tagattggca ctttagtata aggtgctttt tttataggca aaaacagtac
     7621 atttatttct gttttattat cattcatcta ttagaatata ctttagttaa gaatgtgtta
     7681 ataagcagtg ttcacattct tgataaaatg tggaataata tgtattttta ctaagacgga
     7741 aatgaaataa ttaaaccatt aaataatatt ataagatgat gaataagtag ataaatcaaa
     7801 ttttgtagga ggatattatg gaaaaatata tatggttgtt tccattactt tttatttttc
     7861 atgatatgga agagataata ggttttggaa tatggcttaa aaaaaataaa tcaatgcttg
     7921 ataagaagta tccatttatc agtaaagttt atgaaaatta tagtactgaa ggcatggcat
     7981 ttgctgtgtt tgaagaattt attttatgca ttattttctg tatacttaca gttataacag
     8041 agaatcagta tgtatatcta ctatggttag gtagttttat agcatatacc ttacatttgg
     8101 taattcatat agggcagtca atcataataa gaaaatatat accttcactt attaccagta
     8161 tcatatgctt accaataagc atttggtgta taagtaagag tatttatata gttgattgtg
     8221 aaatgagtac aacaattctg tacagtataa ttggcattat aattgtagca ttgaatttaa
     8281 agtttgcaca gtcgctgatt ggaaaattta caaagtggat gtcaaacata taaattttag
     8341 tttattggac agttcaagga atcagaaatt ggaattttat agtattaata ttttatggta
     8401 ttaatataat gtataaaatt ttatagcaat gtatttcata agtataaagt gactagaatt
     8461 ttaagaaata tacaaattct ataaaaacta aataatttca agtatatttt aaaaatctta
     8521 tttttcacaa gtttaaaatt gtaaacgatt tcatcaagtc tatgaatata cttttaacat
     8581 gttatagtaa atctcaatat aaatttacat ttggttcttt gtgtgttatt ttaataataa
     8641 attaagtgaa aatgttattt tacattaata tataatatct ttgcataaga gtgaataata
     8701 taaaagagaa ataattgata ataaaaactt aacaaaaaaa ttaatacata aaaaaaaata
     8761 aatataatgt gttgacattt aattgaaata ttcatacaat aaaattgaaa ataaatattt
     8821 ccaaatgaga ttagcgagag agtcctttat acgaaaaggc gccgaagaag aaatttaaga
     8881 aatgccaatt tttagagaag ctttcaggca aaaatatcgc taattgatgg gactctggag
     8941 agacttaagt taagatacta taagtcaccg aagaagcaaa ataatattaa attattaaat
     9001 ctttcaggta tacaaacagg gatatatagg ctaggattat tttccatgct gatatatctt
     9061 ttttttatga aaaagtacag gaaataatat caaagttact taaatacagt aggtctaatt
     9121 ttctagggag ggttatatat gaatgaattg aaaagagtat ctttgtacaa tatacacaaa
     9181 gagcttggag caaaacttgt tgaatttgct ggttgggaaa tgcctttaga atatgagggt
     9241 ataaataaag agcatgagaa ggtcagaaaa agtgcaggta tatttgatgt atctcatatg
     9301 ggagaggtac agataaaggg agcagagtca gaaaaattta tacaaaacct agttacaaat
     9361 gatataagcg cattaaagat aaatgatatt atatatacac ctatgtgtta tgaaaatggt
     9421 ggagtagttg atgatttatt aatatataaa tttggtgaag aagattattt attagtaata
     9481 aatgcaggta atatagataa agatgtagca tggataataa aacaaagtga aggatacaat
     9541 gtagatataa aaaatatatc tagtgaagtg agtcaattag caattcaagg acctaaagca
     9601 gaagaaattc ttcaaaaaat cacagatata gacttaaact caataaaatt ttataagtca
     9661 attccatcaa caaaagtatg tggttgtcct tgtttagttt caagaacagg atatactggg
     9721 gaagatgggt ttgagatata ctgtaaaaat aaatatgtag aaataatttg gaatgaagtt
     9781 ctaaaggttg gaggagaaga tatatgtcca gctggtctag gttgtaggga tactttgaga
     9841 tttgaagcag ctctacctct atatggacat gaaataaatg aacacatatc tccaattgaa
     9901 ggtggtttat ctatctttgt gaaaacaaat aaagaatcat ttattggcaa atcaatttta
     9961 tcaaaagaaa aagaaagtgg agctaaaaga aagttagtag gatttgaaat gcaaggtaaa
    10021 ggaatgccta gaaatggata tgatataagg attggtgata aaacagttgg atttgtaaca
    10081 acaggatgtg catctccaac gacaggaaaa attttaggta tgggaattat agattctgaa
    10141 tatgcaaaag taggaaatga aataggtata gcaatcagaa aaaaagttgt tcctgctgtg
    10201 atagttaaga aaccttttta taaaaaacaa tataaaaaag ataatattat attaaacaaa
    10261 gaaaataaat tttcatatat acctgctaca agtgaagata aatcaaaaat gttaaaagta
    10321 gttggattaa attcggttga tgaattattt tcagatatac cagaagaagt aaaattaaaa
    10381 agagatttaa atttggaaat tggaaaatct gaattagagg taagcaaaat agtaaaaaga
    10441 ttgtcagaag aaaatttaag tttagaagac ttgacttgtt ttcttggtgc tggtgcatat
    10501 gaccattata taccatcaat tataaaacat ataacttcaa gatctgaatt ttatacagca
    10561 tatactccat atcaagctga aataagtcag ggaactttac aagtagtttt tgaatttcaa
    10621 tctatgattg cagaaattac aggtatggaa attgcaaatg catcaatgta tgatggagca
    10681 actgctgcaa tagaagcatg tataatggca atgaatcaga ctagaaaaag taaaatagta
    10741 gtgtcaaaaa ctattcatcc tgaaactttg tcaatattaa gaacttactt acaatataaa
    10801 gattgtgaga tagtagaaat tgacttttgt aatgaatatg gcactacaga tatagaaaaa
    10861 ttaaaagctt ctgtagacaa agatacagca tgtgttctta tacaaactcc taatttcttt
    10921 ggaattattg aagaaatgga agaaatagaa aagataactc atgaaaataa ggctatgtta
    10981 attatgagtg tagacccaat atcattagga gttttaaaaa caccaggtga gataggtgca
    11041 gatattgttg ttggagaagc tcaatcactt ggaaaccctc ttaattttgg aggtccttat
    11101 gtaggatttt tagctagcaa atctaaatat actagaaaaa tgccaggaag aatagttggg
    11161 caaagtttag atgtagaagg taaaattgca tatgtattaa ctttacaaac tagagaacag
    11221 catgtaagaa gagaaaaagc aacttctaat atatgttcaa atcaagcttt aaatgctctt
    11281 gtagcttcaa tatatatagc tactatgggt aaagaaggat ttaaagaggt tggtatgcag
    11341 tctatgaaaa aagcacatta tacttacaat aagcttgtac aaactggaaa atataagcca
    11401 atatttaaag gtaagttttt caaagaattt gctgtacaag gaaatcttaa tatagaaact
    11461 ataaatgata aactcttaga agaaaatata ttaggtggtt ataatttaga atacaattat
    11521 ccagaattaa aaaattcaac tttgctttgt gtgactgaga aaagaagcaa agaggaaata
    11581 gataagttag ttggaataat ggagggatta taatgaaaga atacaatagt ttgttgatag
    11641 atatatctaa aaaaggtagg aaagcatact cacttccaaa gttagatata gatgatataa
    11701 aaatagaaaa tatgattgac caagatatgg caagacaaag tgagttgaat ttgccagaag
    11761 ttggtgaatt agaattagtt agacattata cattattatc aaataaaaac tttggagttg
    11821 atacaggttt ttatcctttg ggttcttgta ctatgaaata taatcctaaa ataaatgaag
    11881 atatggcagc acttccaaac tttactggaa tgcatccata ccaatcttcg gatacagctc
    11941 aaggttctct ttctttgatg tatgatttat caagaagact tgcagaaatt acaggtatgg
    12001 atgaagttac actacaacct tcagcaggtt ctcatggtga gtttacaggg cttatgatta
    12061 taaaagcata tcatgaaaat agaggtgacc ataagagaac caaagtgata attccagatt
    12121 ctgcacatgg aacaaatcca gcaagtgcag ctatggcaaa ctttgatgtg attcaaatag
    12181 catctgataa aaatggagct gtagacataa atgcattaaa agaggttttg aatgatgaag
    12241 tagcagctct tatgcttact aatccaagta ctcttggttt atttgaaaaa aatataaaag
    12301 aaatagctac tcttgtacat gaagcaggag gacttttgta ctatgatggt gcaaacatga
    12361 acgctattat ggggataaca agacctggag atatggggtt tgatgtggtt catcttaatc
    12421 ttcataagac tttttcaact cctcatggag gtggaggtcc aggagctggt cctgttggag
    12481 ttaaaaaaga attagtacct tttttaccaa taccagtaat tgaaagaaaa gaagataagt
    12541 atgtactaaa ttatgataga gaaaaatcta ttgggaaaat taaaaatttc tatggtaatt
    12601 ttggagttct tgtaagagct tacacatata tactaactat gggaagggat ggacttaaag
    12661 aggctagtga aatggcagtt ttaaatgcta attatataaa ggaaagtata aaagatgatt
    12721 atattttgcc aatagatacc ttgtgtaagc atgagtttgt attaggtgga ttgccaagag
    12781 gagaggccaa tataaaaact atcgatattg caaaaagact actagattat ggataccatc
    12841 ctccaacaat gtactttcct cttattataa atgaggcact tatgatagaa cctacagaaa
    12901 gtgagagtat agagacatta gatagtttta tagaagctat gaaaagtgtt gctaaagagg
    12961 caaaggaaga accagaacta ttgaaaactg ctccacataa tactttggtc aaaagagtag
    13021 atgatgcaag ggcagtaaag aaacctatac taacatggtc acaaagataa atagttaaaa
    13081 agtagagatt taaagtataa agagaattaa atttgtaaga aaaagctatc taacttaatc
    13141 tagtttagat agcttttttg tatattagta tgtatggtat tttctatata aagttttaaa
    13201 ttatattagt gcttatatag aatattgata tgatgattat gtatagatat taataagaat
    13261 tgttgaattt a
//
LOCUS       DAMKMI010000112        13256 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMKMI010000112 DAMKMI010000000
VERSION     DAMKMI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.116"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            217..1635
                     /locus_tag="PC429_002822"
     CDS             217..1635
                     /locus_tag="PC429_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF3848311.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
     gene            1711..2523
                     /locus_tag="PC429_002823"
     CDS             1711..2523
                     /locus_tag="PC429_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3848312.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            2946..3563
                     /locus_tag="PC429_002824"
     CDS             2946..3563
                     /locus_tag="PC429_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF3848313.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(3720..4172)
                     /locus_tag="PC429_002825"
     CDS             complement(3720..4172)
                     /locus_tag="PC429_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848314.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRIFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            4620..5978
                     /locus_tag="PC429_002826"
     CDS             4620..5978
                     /locus_tag="PC429_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3848315.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGLLRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            6251..6631
                     /locus_tag="PC429_002827"
     CDS             6251..6631
                     /locus_tag="PC429_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3848316.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            6756..6956
                     /locus_tag="PC429_002828"
     CDS             6756..6956
                     /locus_tag="PC429_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848317.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            7152..7880
                     /locus_tag="PC429_002829"
     CDS             7152..7880
                     /locus_tag="PC429_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3848318.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            7902..8819
                     /locus_tag="PC429_002830"
     CDS             7902..8819
                     /locus_tag="PC429_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF3848319.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTEAGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            8961..10145
                     /locus_tag="PC429_002831"
     CDS             8961..10145
                     /locus_tag="PC429_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3848320.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPSNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGVDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            10252..10440
                     /locus_tag="PC429_002832"
     CDS             10252..10440
                     /locus_tag="PC429_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF3848321.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            10589..11239
                     /locus_tag="PC429_002833"
     CDS             10589..11239
                     /locus_tag="PC429_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF3848322.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKAKEAVEKYREYFADKGIFENKIYENMKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEEISESEPTYIVENVESIKDILL"
     gene            11342..12280
                     /locus_tag="PC429_002834"
     CDS             11342..12280
                     /locus_tag="PC429_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF3848323.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            12306..13157
                     /locus_tag="PC429_002835"
     CDS             12306..13157
                     /locus_tag="PC429_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF3848324.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGAL
                     IKNDSIESKSESVKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKSFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         5290 a         1277 c         2341 g         4348 t
ORIGIN      
        1 taataaaaaa aaattcaaat tataaatatt aaatgaatat aattaaaaca aatatataaa
       61 tttaagtata acactcacat tttaatgtat tgtgaaattt ttgttgaact ttatgtacca
      121 taacttataa aatttatgtc atgttatatt tgaataagta ataaaattgt agatgtaaat
      181 taatctacaa aatttatatg aaatggagtt gatgctatga cagaattaat gattataagt
      241 gggttagtac tattgatttg tataacttct agcaaggtat tgtataaatt tggagtacca
      301 atattattga tttttatctt actagggatg ttatttgggt ctgatgggat ggtaggaatc
      361 tattttgata actatgaact gacaaaacaa ttatgttcta ttggactaat atttataatg
      421 ttttttggag ggtttggaac aaactggaat atggctaaac ctgttgctat accttctata
      481 ttaatgtctt cactgggtgt aataattacg gctggggtta caggtatgtt ttgccacatg
      541 gtaataggaa ctagtttact tgaaggttta ttaattgggg caattgcagc ttcaacagat
      601 gctgcatctg tgtttgctgt gttgcgttct caaaaactaa acttaaaggg ttcacttgca
      661 tcattattag aagttgaaag tggaagtaat gacccaatag cgtatatgtt gacactaatt
      721 cttttgacat tgatgaataa ttctggggta gaacttattg caccaatggt aataaaacaa
      781 atagcttttg ggttagctat aggatttata ctagcaaaat tatctgttta tattttaaga
      841 cattcaaact tcgagataga aggattttat actatattta taacagctat tgcaatactt
      901 tcttatgcat ttagcgagta tcttggagga aatggatatt taagtgttta catttcaggt
      961 attattatag gaaatagtag gatacctctt aaaaaaagtt tggttcattt ctttgatgga
     1021 atttcatgga ttatgcaaat aatgttattc tttatgttag gattgttatc tttcccttct
     1081 aagttaccaa atgtatttgg tatagcaatg gccatatcta tattcatgat agttattgca
     1141 agaccattag caacatttat agttctagca aaatttaaat tctcaaataa agaaaaatta
     1201 ttcatatcgt gggtaggtct tagaggtgcg gcttcagtag tttttgcaat ttatgctgtt
     1261 actcaaggag tagccataga gaatgacata ttccatataa tattttttat agcgttgctt
     1321 tcagtaggaa tacaaggttc attgatacca actgtagcca gaaagttgga tttagttgat
     1381 gataatacac ctgtattaaa aacatttaat gattatgatg gagaaattag ctctaaactt
     1441 atcgaagtaa atgttgatga gggtaataaa tgggctaata agagtataat agattctaat
     1501 ataccagacg atatattaat agttatgata aaaagaaagg aacaggtttt aatacctaaa
     1561 ggttcaacca ttataaaaac aggggatatt ttggtattga gtggagatag aattgaagaa
     1621 ttaatacaat tataatttat tttgtattga ctttaaccta aggggaaagt ttaaacttaa
     1681 attaatcaaa tctaatgcgc aggtgatgag atgttttcta taggaatgtt ttctaaaata
     1741 aataaaataa ctacaaagac attaaggtat tatgaagata tagggttgtt aaaaccagaa
     1801 tatgtagatg aatttacaaa atataggtat tatacaacag agcaattacc taaattacat
     1861 cagataataa ctttaaaaca aatgggttta tcattagctg agataaaaaa agctgtagaa
     1921 aatcctgaag aagttgaaaa tattttaatg aataaagaaa aagaaatgtt ggatgtaatt
     1981 aaaagtgaag aatataaaat aattaaacta cgtaattacc ttatgaattt gaaaggagaa
     2041 gtcaatatga atgatgtaat tattaagtct ttaccaaaag ttaaagttgc ttctatgaga
     2101 aaagtgttaa aggggtatga tgaacttttt aatctatttc caaatgtaat ggctgaagaa
     2161 atgatgaaaa cagggtgtac atgtgcagtt cctgattatt gttttaatat atatcatgat
     2221 ggtgaatata gagagactga tatggatgtt gaaatgtgtc aagctataaa tgatttaaag
     2281 gaagatacgg atatattaaa atttaaagaa ttagatgcag ttgaaaatgc agtttgtata
     2341 ttacacaatg gtccatattc aacattagga aaaacttacg cagatgcatt taaatggata
     2401 gaggacaata aatataaagt ggttggaaat cctagagaat cttatataga tggtatatgg
     2461 aataaagaga gtgaagaaga ctggttgaca gaagttcaga taccagtgaa acgcatgtta
     2521 taatatgtct acaaagtata aataagaata aaaaaggtgg tgtctcaaag taaacatttt
     2581 agtttatgag acactctttt tcatatgaaa tcaagtatat ttttataatt acgattatga
     2641 aaaaaggctt atctatattt ttagacagcc tcttattttt ttattataat agttttctaa
     2701 agatgctcta tttataaatt tttattaaag actttataaa taattgtatt taatatacaa
     2761 aatatatata aatattgatg ttttatatat aaagttgtat atttttttaa ggatataaag
     2821 taaaaaaata tacaatatca agaaaaaaag atattgggta tttaaagtaa tactttattt
     2881 aattttaagt aaatcgggta tctacatata tagatactaa tttcaaagaa taggagggtg
     2941 tttatatgga aatattcgca ataggacatt caaattatcc atatgataag cttattaata
     3001 tgataaaaaa atatgacata aattgtgtag ttgacattag agaaactcca tattctaaat
     3061 ataatattca atacaataaa gaggcattta acgaatctct tagaaattcc ggatttttat
     3121 atatatacat gggaaaagaa tttggagcta aaagaactaa taaagatgta tatacgcaag
     3181 aaggttatgc agattttgag aaagtagcaa aagaagatat ttttttaaac ggaatagaaa
     3241 gattaaaaaa gggttgtcaa atgggttata ggatagtttt attaggagct atgcaagaac
     3301 ctataagatg tcatagaagt atcttggtgg gaaaagtatt aaataaagaa ggttttgatg
     3361 taaaatatat aatgcatgaa ggaaatttgg catatcagga agatatagag gaatcattat
     3421 tggataagta ttttagtgat agaaagcaac tgagtattga caatttatta gggagtgcat
     3481 taacgagaga agaaatgata caagaaggtt ataagctagc taataaagaa ataggatata
     3541 gaactgagaa actaggtaaa taaaaatata caagtagata aaatacagta cttatcttaa
     3601 gtaaaataat attatgaatt tataatagct aggcatgatg tgccaattaa aaatattcta
     3661 gttatttaat aatatcatta aatttagggt attttttaat agcacatcat gttgcagttt
     3721 taaaaaataa aatctctaat attgccataa tcaactgttc tattaagagt aggatgacta
     3781 gatataaatc taactgtaat ttttgaattt aacaaacaag gatttgaaat taaaaatatt
     3841 ctatctccat tgacaataga agtaatagct actggcaaaa tatctacaaa tgtatcatat
     3901 ctgtttttag caatttgata aggataattt cctacaaaaa caaatagatt taaatcatat
     3961 atattatcag taaaccaagc ccattgacca taaactttgt cgatattcat tgtgcctaaa
     4021 ccaagtgtat caaagtatgt tgctatagtt aaataaatat tttccatgtt ttctaaatat
     4081 gaaacagtat atcttctgaa acgtatgggt gctgtggaag aaataggata aaccatattt
     4141 acatttagat tatttgcatt taaattaacc aaagtatcac atcctaaaat tagtctataa
     4201 aatacaatat ttaaatggat gcgattttgt tacatctaaa gtgtaatatt ttatatattg
     4261 aaaaagtggt aaaagtttgg atgattaaat aatattataa aaaatatata aaatgtactt
     4321 attgttattg ctacacgata gtgatacaat attaacgaaa tatagctact tttaataata
     4381 acggaaaata tttatattaa attaataaat attttaaatg tggtatccat aaaaaataat
     4441 aaacataaga taattataaa gatatactgt gttcaaatga tattaaacaa gatttatatt
     4501 atttgaaagc agtatattat aatgcgaaca atacaattct aaactttgca ttgtcaattt
     4561 acagttcgat aaactaatta aaaattaaat ttatataaag agggaaaaga ggaataggga
     4621 tgtttataag gaaaaatgtt ataaagttag caattcctat aatgatagag cagacgtttg
     4681 tcatgctttt aggagtattt aatacaatga tggcaggtca tattggggaa gaagctgtat
     4741 ctgcagttgg aatggttgat tcaataaaca atatatttat atctgtattt gctgcactat
     4801 ctgttggagc tactgttgtt gtagctcaac aaataggaaa aaaagatggt gaaaaggtga
     4861 atgaaactat taagcaggct ctagtgtctg gagtaatatt atcctttaca ataacaatat
     4921 tgatgtgggt ttttagaact ggtgtaatta atttatttta tggttccgca gaagaattgg
     4981 taaaatcaaa tgcaaaatta tacatagagt ttacattatt tacgtatcca tttattgcga
     5041 cacaacaaat agcaaatggt cttttaagag ggtgtggaga tgtaaagaaa cctatgtatg
     5101 tgactatgtt tatgaatata gtaaatgtaa cattaggata tatacttata tatggtattg
     5161 atttaaatcc tctaggaata aatttgcaaa caccatccta tggcataaca ggtgcagcta
     5221 tatctattgc tatagctaga ataattggat gtatagtaat tataagagct ttatttaaag
     5281 gaaacgaatt tatatctatg ggtaaaatat ttccatttaa gattgatgtt gaaactcaaa
     5341 agtctatatt taatatagga ataccagctg gagttgaaca actacttttt aatgcaggta
     5401 aattgattgt tcaagtattt atagttacta tgggaacatc agcaattgca tccaatgcaa
     5461 taggaatgtc aattgcatcc ataataaatg taattgggaa tgctttagct ttgtctgcta
     5521 caaccttggt gggacagtat ataggacgtg gagatattaa aggagctaag agtactttat
     5581 tatatttaac taaatttgca acaatttgtt tatttatagt aggagctata tttataccaa
     5641 ttgcaccttg gatttcaagt atgtatactc aaaatgctag tgtaattgac atctcaacac
     5701 aattaattcg aagtgattgt ttagctatgg taatatggcc tatatccttt gtattatcag
     5761 cagggctaaa gggagccggt gatactagat atacaatgat gactgcagta ataggtatgt
     5821 ggatttttag aatttttaca ggttacgtat tgggggttgt attagaattt ggagtgttag
     5881 gaatttggat aggtatgtat actgattggc ttgttagagg gactttatat tgtttaagat
     5941 taagaggaac aaagtggcta aaacacaaag ttgcatagaa tatatgaaat cacttttaga
     6001 tggataactc tatttagaag tgatttttta tgtagttatt atcataatta taagtatgat
     6061 atataaggtg attaaattac ataaataaag gctataatag tatggatatt ataaattata
     6121 tattgagcag aattacataa tttggcgata aatattagaa atatatatct atagaaaata
     6181 atatatatgg gtatattatt aaatataaaa aatattaggt atttatttaa taataaaggt
     6241 gggtattgtt atgataaata aaataggaaa aataactctc tatgtaaata atcaagagga
     6301 tgcaaagtta ttttggacag aaaaattgaa ctttgtagta acttatgaaa aggaaatggg
     6361 tccaaatatg aaatgggtgg aagtagcacc aagtaaaaat gaatttacaa catttgtgtt
     6421 gtatgataaa aatctaatga tgaagcaaaa caaagagata aatgtatctc atccatcaat
     6481 aattttaagt acaagagata ttaatggaac ttatgaacaa atggcaaata acgaggttga
     6541 ggttggggaa ttaatggaga tgccatatgg aaggatgttt tcttttaaag accaagatgg
     6601 aaatgattat ttggtcagag aggataaata attttacaaa gtattaaatt tactcaatat
     6661 ctgatataat tagagacaat aattattata aactagtcta ggattagaat tatataatta
     6721 aaaattaagt gtaaattttg agtagaggtg ataaaatgtt taagaaaatg gcagtactaa
     6781 aagatatagc aactaaaata ggtcgtaaaa aagcgtatga actattagaa atggttgaag
     6841 gtaatgatgc ctttgtagct gaggtaaaga taaaaaagaa tggaatagaa tctaaaaaag
     6901 aagaaattat gttaaaagat aatcaaaaaa taatattaga gtatatagaa ggttaaattt
     6961 taaatacata taagttgata tactaaattt ctgagatgag tttacataaa actcatcttt
     7021 tttacttttc aataactttg ttttattatt taatatacat tttatctcct tagagttgct
     7081 gatatataaa aattactgat ataataaaag aatggatatt aatgtaatat tttatagaaa
     7141 agaggggtat tatgcttgaa ataaaaaatc tttcctttaa tgttgagtct aacaatgagg
     7201 aactaggaat aataaatgat gttagccttt catttgagag gggaaaactt attgttatca
     7261 caggtccaaa tggtggagga aaatctacaa ttgctaagct tattatggga attgaaaaag
     7321 ctacgagtgg tcagataatt ttagatggag aagatataac aaatcttagt attacagaaa
     7381 gagcaaaaaa aggaatagga tacgcatttc aacaaccacc aagaataaaa ggtatgacag
     7441 ttgaaaattt acttacactt gctcatggaa aaccattgtc tactgatgta tgttgccaat
     7501 accttacaga tgttggattg tgttcaaaag actatttaaa tcgtgaagtt gataactctc
     7561 tttctggagg ggaaatgaaa agaattgaga tagcaactct ttttgctaga gatttaaagg
     7621 tttctatatt tgatgaacct gaagcaggga tagacctttg gagctttgga aaactcaatg
     7681 aaagttttaa gaaaatacat gaagagtcta atcagactat aatcataatt tctcaccaag
     7741 aaagaatatt agagttagct gatgaaataa ttgttctaca agatggttca gtaaaaagcc
     7801 atggaacaaa agaggctata ttacctgaaa tcatgtgtca agttaattca agttgtgaat
     7861 taatgaaaga catgaattaa ttagaaagga aggcaaatta aatgaacaaa atagatttaa
     7921 atcttttaga aaaagttgct gaacttcgtg gaactccatt aggagcatac aatatacgta
     7981 aaaatgggca aggagtagag agacattcaa ctgaaaatgt aactataatt cctaaaactg
     8041 ataaacctgg aatagatatt atagtaaagc caaatacaaa aggagaaaat gtacatatac
     8101 cagtaatagt aacagaggct ggtgtacagg atatggtata taatgatttt gaaattggag
     8161 aaaatgcaga tgttgttata attgctgggt gtggtataca taactgtggt tgtgatgata
     8221 gtgaacataa tggtatacat agatttttct tacgtaaagg agctcgccta aaatatgtag
     8281 aaaaacatta tggtgaaggt gatggagaag gaaagagaat tttaaatcca gttactattg
     8341 tcaatatgga tgaggattca tattgtgaaa tggaaacaat tcaaattaag ggtgtagatt
     8401 caactaaaag agaaatggat gcaaacctag gtaaaggagc taaaatagtt gtatttgaga
     8461 aattattaac tcatggtaat caagttgcag aatctaatat gaaggttatc ttagagggtg
     8521 aaaactcttc tgctcaagta gtttctcgta cagttgggca agataattct aagcaaaagt
     8581 ttaatccttg tgttattggg gattcccttt gttctgcaca tgttcaatgt gattcaataa
     8641 ttatgggaaa ttctcaaatt agtgctatac ctgaaattgc tgcaaatcat ccagatgctc
     8701 ttttagtaca tgaagcagct attggaaaga ttgcaggaga ccaaatacta aagatgatga
     8761 cattaggtct tactgaagaa gaagccgagc aacaaatact taattgcttc ttaaagtaaa
     8821 ttaaagattt tatttaattt aatataatta atatagtata atatagtgaa taagtctaaa
     8881 atatttttat ttttaggctt attttcttta tgtaagaaaa gctattagag aaattatatt
     8941 gtaattagga tgtggaataa atgaaaaatt tacttataag aaatgggaca ataatatctc
     9001 caacagatgg tatcaaattt gaaaaaagag atatcttggt tttacatggg aaaatatatg
     9061 ctattggatt gaaccttaaa gagaaaataa gaaaagaaaa attacttaca aataaagagt
     9121 tcgagataat aaatgccgat aatatgtata tttccccagg atttatagat gttcacaccc
     9181 attgttataa gagaaaatta cctacaggta tggattctga ttcaattgga attgaaaaag
     9241 gttctacagt tatatttgat gcaggtacag ctggtccttc aaactactat gattttaaaa
     9301 aaaagtatat ggatgagtgt aaaacagaag tatattcttt tttaaatgtt acaaatgagg
     9361 gactaaatat attgaatgaa tcaacttctt tagatgttat aaattttgac aaggttgagt
     9421 cagttgtaga aaaaaatata gaaaagataa agggtataaa agtaaaagcg agtaaatttc
     9481 aatctaatga aagtggtata gatataatta agaaatcaaa gcaagttgct aaaaaattaa
     9541 atttaccctt agtggtatat ataggagagt atccatcata tatagatgaa gtaatgaata
     9601 ttttgggaaa aggagatgtg gttacaccag tatatggaaa tagtacaaat ggaatcctta
     9661 aagaatctgg cacattaaga aaatcagtaa taaatgcaaa gaatagagga gtattgttcg
     9721 atgtatgtca tggagtggac caatttgatt ttaaagtttt taaaaaagca ataaagcaag
     9781 gtttagagcc tgacctaata agtactgata tgcatataaa aaacatgaaa gacgtaaact
     9841 attctctttt aaatgttata aataaaatta tggaacttgg gatatcccta gaaaagtgta
     9901 tagaaaaagt aactgaatac ccaacaaaga ttttcgattt acatggaaat aaaggcagaa
     9961 taaagattgg tggagaaggt gattttacaa tattcacaat ggaggagtgt aatgatatta
    10021 taaaagacta caataataat gaacttgttt taaataaaaa attaagattg caatatactg
    10081 taaaatcatg gatgaaaagc agtgaagttt atagacatgg atacgaaaat actataataa
    10141 actaaacaat agtgataaaa taataaatat atgtttaaat tggtatatat tggataaata
    10201 tctaaggaaa caacaattaa aaaataataa ttttagggga ggtattttag tatgaaagca
    10261 tttgttgata aggatgtatg tgtagcttgt ggagcatgta taggaacatg tccagacgtt
    10321 tttagtatga gcgatgaagg gcattcagta gcaattgaag gagaaatacc agcagattta
    10381 caagatttat gtaaagaagc cgaagaagga tgtccagttt cagctataac agttaaatag
    10441 agctgtttaa atttattttt aaaataaata tataagattt aattggtact taagctatag
    10501 tattagaaat ttctattact atagcttttt aaaatagata aacttaatat ataagatatt
    10561 actttttaca aatatggagg actgaaatat gaaaaaaaat tatgaaatag tattattcga
    10621 tttagatgga acacttacag accctaaaga aggaataaca aagtctattc agtattcttt
    10681 aaatagtttt ggcataaaag aagatttgga aaacttagac caatttatag gaccaccatt
    10741 acatgatact ttcaaggagt attataaatt tgaagataaa aaagctaaag aagctgttga
    10801 aaagtataga gaatattttg ctgataaagg gatatttgaa aataaaattt atgagaatat
    10861 gaaagagata ttagaaatgc tctataaaaa tggtaaaata ttattagtag caacttctaa
    10921 gccaacagtt tttgcagaaa ctatacttag atattttgat atagatagat attttaaata
    10981 tatagcaggt agcaatcttg atggtacaag agtgaataaa aatgaagtta tacaatatgt
    11041 actagaccta tgtaatgtaa aagataaaga taaagttatt atggttggag atagaaagta
    11101 tgacataatt ggagctaaaa aaataggtat agattctatt ggagtattgt atgggtatgg
    11161 ttcttttgaa gagattagtg aaagtgaacc tacttatatt gtcgaaaatg tagagtctat
    11221 aaaagatatt ttgttataaa tatcttttat agacaaaaat attacaaaat ggtgtcgcat
    11281 atttaaaaat atgatattat agttaaggaa gtcatttaaa aaagaggagg atgcacttaa
    11341 aatgaaatct attttaatta tgggtggaag cgacttcata ggttctgcac ttgctaagcg
    11401 cttgattaaa tgtgggtatc aaattgatat acttacaaat ggtaaaaagg aaatagatta
    11461 taatggtttt aaagaacatc taatttgtga tagaaaagtt cgaaaagata tggaaaacat
    11521 tatcacagga agaaaatatg attacatata tgatatgaca gcatatacaa aagaagatgt
    11581 aagtaatctg attgatttta taagcatgga taacttaaaa aagtatatag tattgtcagc
    11641 aggagcagta tataaagata gtggaagaaa tataaaagaa gaaaatgaga aaggtgaaaa
    11701 tgaaaactgg ggaaaatatg ggttaaataa aaaagaggct gaagatttta ttataaatag
    11761 ccctatacca tatataatta ttagaccaac ttacatatat ggtgaaaata ataatttata
    11821 tagagagtat tatttctttg aaaaaataga aaaaaatgaa aagataccag taccaaaagg
    11881 aaaacaagtt agtaatcaat ttatatatat tggggatttg gtaaaggtat tggaaagcat
    11941 aatgaaaaat cctcatgtaa gagaggccta taatgtgaca aatccacagt taatttcttg
    12001 ggatgacctt atatatacat gtggagaaat tattggcaaa gaacctataa taaaatatgt
    12061 agatatggaa aaagtagagt ttagagaaag aacgtatttc ccatttagaa atatagactt
    12121 taatcttgat ataaataagc ttattgaaca tgggctttat ataccaaacg tgttactaaa
    12181 agaaggtctt acagcaacat ataaatggtt ttcagcaaat aagccaaaaa tgcatgacag
    12241 aaaaatgaac aaagttgagt ctgtaatgca aatagtttaa aaaaataaat aagtaggtag
    12301 atattttgaa ctcaaaaatt aagtattttt taaaattttt tatcttaata tttattttga
    12361 tttttatata ctcagttgcg atagagccga gtctattaat tgtgaaaaaa tataatataa
    12421 gtgaaaatgg agcattgata aaaaatgatt caattgagag taaatctgaa agtgtaaaag
    12481 tagttcaaat ttcagatact caaataggta gtttttattc aactaagaat ctcaaaaaag
    12541 tagctaataa gattaacact ttgaatccag atataatagt atttactggt gatttaattg
    12601 attactccaa taaaaatcca agtgtagatg aaattacaac tattttatca agtatgaatg
    12661 ctaggttggg aaaattttca gtatttggca atcatgatta tatgtataaa cttcctaggt
    12721 attataggca gattatgaaa aattccaact tcaatctttt agtaaatgaa aataaaaaaa
    12781 taaaattaaa agatgataaa tatattaata tattaggtgt tgatgaaata ttaaatggaa
    12841 atcctaatat aaaatatctg gagagtcaaa tagataataa aagttttaat ttattgttag
    12901 cacatgaacc agatttagta gatatgctta gtaaagatac aatgaattta gttctatctg
    12961 ggcatagtca tggtggtcaa ataagattac ctattaaggg agcattagtt actcctccat
    13021 atggaaggaa atatactaaa gggttttatg atataaatgg aaatcattta tatgtgagtt
    13081 cgggtcttgg aagtacaaag ttaccattta gattttttaa tataccagag ataatagaat
    13141 ttaatattat aatctaacat aaaatattta gaaaatataa ttttgctttt tataatatgt
    13201 agatgtatat actgtaaaaa tggattaaac cagtagattg aatgaattaa tagatt
//
LOCUS       DAMKMI010000113        12974 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMKMI010000113 DAMKMI010000000
VERSION     DAMKMI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12974
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.117"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            162..1526
                     /locus_tag="PC429_002836"
     CDS             162..1526
                     /locus_tag="PC429_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3848325.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            1548..2039
                     /locus_tag="PC429_002837"
     CDS             1548..2039
                     /locus_tag="PC429_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF3848326.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2311..2982
                     /locus_tag="PC429_002838"
     CDS             2311..2982
                     /locus_tag="PC429_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF3848327.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMKRLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            3067..3933
                     /locus_tag="PC429_002839"
     CDS             3067..3933
                     /locus_tag="PC429_002839"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF3848328.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3966..5468
                     /locus_tag="PC429_002840"
     CDS             3966..5468
                     /locus_tag="PC429_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF3848329.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            5598..6050
                     /locus_tag="PC429_002841"
     CDS             5598..6050
                     /locus_tag="PC429_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF3848330.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6210..7013
                     /locus_tag="PC429_002842"
     CDS             6210..7013
                     /locus_tag="PC429_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848331.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            7071..7958
                     /locus_tag="PC429_002843"
     CDS             7071..7958
                     /locus_tag="PC429_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF3848332.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7977..8825
                     /locus_tag="PC429_002844"
     CDS             7977..8825
                     /locus_tag="PC429_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3848333.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8888..9577
                     /locus_tag="PC429_002845"
     CDS             8888..9577
                     /locus_tag="PC429_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF3848334.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(9907..11097)
                     /locus_tag="PC429_002846"
     CDS             complement(9907..11097)
                     /locus_tag="PC429_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF3848335.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYAWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLDDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSERGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLRDSEIGYYIDTPKTKNGKRKIPLTE
                     RAYQALQRILKNRVKAQPLVVGGYSNFLFLNREGLPKVAGNYVGMVRGLIKKYNKYHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(11175..11378)
                     /locus_tag="PC429_002847"
     CDS             complement(11175..11378)
                     /locus_tag="PC429_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF3848336.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(11760..12008)
                     /locus_tag="PC429_002848"
     CDS             complement(11760..12008)
                     /locus_tag="PC429_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3848337.1"
                     /translation="MKHTFKKPSYYLLSQAMDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(12012..12431)
                     /locus_tag="PC429_002849"
     CDS             complement(12012..12431)
                     /locus_tag="PC429_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3848338.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEKEISFC
                     ELPEIMVENLAVWDDYETDYTIFNVCGNDIRVYDDELAEALKQLSERNRETLLMYYFL
                     EMNNEEIAKKQNISRSGVFQNRHNSLALMRKLLKEKR"
BASE COUNT         4780 a         1604 c         2302 g         4288 t
ORIGIN      
        1 ataaagttcg cattaacaaa ctatttaaat atcaagacct gttaaaaaac aacagcaata
       61 aacaaataaa atagataaat atatccaatt aaggtataat taagttttat atgatatagt
      121 tatatttgat aatttacaat agtaaaactt ggagggaaaa aatgggtgaa aagaaaataa
      181 ataatatgaa ttattcacat aataattatt ccaaaaagac aggtgaacca actagaaaaa
      241 taaaaaatag ccatatcact cttaaaagta aagaagaaaa aatttgttct agttgcaata
      301 aagaaaataa tactaaggat aattactgta aattttgtgg aaatgaactt tatgaaatag
      361 catcattaag accacttgaa acaaaactag acttgaaatc taaaataaaa gagctttcat
      421 atcatgcaaa taaaagaggt gtatttttaa caacatttac aaccatattt atattgttta
      481 ttattgcatt gatatttaaa gctattataa ctattcaatt taatgatatt agttatttag
      541 taaatccagc acatataatc ctagctctca acttgggtca aattagtgta tctatgtcta
      601 ccatgatggg ttctgggttt ataaatgcca atataggttt gttgatatta cttataatcc
      661 ctattttggt attattaata tcaaacctaa tttttatgag aaaaagatgt agagattcaa
      721 aaactgtatt agcaaattct ctaggagttg gaatttttta tggattagtt ttagctattt
      781 tatctatttt tactaatgta aaaactagtt cacatagtat gttagaatat ggatatgcat
      841 tagaatatag ttatgaattt tttagtgttc ttttaaatgg attcgtatta gggtttatat
      901 gtacatacat aactacttat aaaaaaagtt atgaaaaaga aaatatgtat ttatctcttt
      961 tttcaaattc tataagaaca ttcatattag gttatgtttt agtattagta atactattgg
     1021 tactaactat atctgatagt agttacttaa atgaactaga tatgagtagt tattcaaatg
     1081 gattaaattt atttactata ctcccacaaa tagctagtta tatgtgggct tttgcaaatg
     1141 gaatttcagt aaccataata aactcaacag tatcaatgtt tactttaagt tcttcatctt
     1201 tatttggaga tactaaatta atgttttatg caatgggagc attgtctatg ttaatattat
     1261 tattaaatgg atacaagcta agatttaagt ataatacaga tagtataaga cctataatag
     1321 tatttagtat atattatgca tttttgatgg gaatattggc cttatttagt acatttatac
     1381 tagatagtaa tattaatttc tttaatacaa ctaattatgg aacaacttta ataatgcaat
     1441 ttaaagtctt acaagctatt gtaatttcat ttgtatatag ttttgtgata tctttaatag
     1501 gatataagct aaattcagct gattaaatat gattggaggg aatttctgtg gaaggaaata
     1561 ctaataataa agtagatgat aaattttcta aggttaagaa aaagtttggt agtggaaaat
     1621 ttaaagttga attatataaa gaaaaatcaa aattaaatag agagattgat gatattcaag
     1681 aaaagaaagc aaagatattc ttggaaatgg gaatactgac atatcaaaaa attagagaag
     1741 attctattaa tgataatagt tttgataaat tctgtgatga gttactagaa cttgataagt
     1801 taatttatga aaaaaatatg gaaattaatg aaatggaaat ggcagaaagt aatgtaactt
     1861 gtgagtgtgg ttatgttgga aatgtaaatg ataaatattg tgctgaatgt ggtaaaaaat
     1921 ttgaattgga aaatgaatgt gatgatttta taatttgtgg ttattgtgaa agtgagatag
     1981 atacagaggc tgaattttgt ccatgctgtg gaagaaaaat tataatggat ttagaataat
     2041 attgggaaac ctatgtatag tatacatggg tttttttatt gaaattattt aaatggactt
     2101 ataacagtag ttatataaaa tgaaaataaa caccaaaaaa atgaaataaa atttctttta
     2161 aatgtaaaaa acaagctaat atataaaata aatcagtaaa aatatagatg cgagaaaaaa
     2221 tatgaaaaaa tattgcaaag aaaaagtttt catgttatta ttaatacata aatcaagaaa
     2281 ataaatttca gaaacgaggt aatttaaaaa atgctagata aggtaaaggg aagattaata
     2341 gtatcatgcc aagctttaga aaatgaacct cttcatagtc cttttataat ggggagaatg
     2401 gctaaggctg ctatggaagg tggagctgtt ggaattagag ctcaaggagt tgaagatatt
     2461 attgaaataa aaaaagttac tggacttcct gttataggta ttataaagag aaactatgaa
     2521 gattcagata tatatataac gccaactaaa aaagaagttg atgaactttt aacaacaggg
     2581 tgtgaaatga tagcattaga tgctaccaat agagttagac caaataatga agacttaaaa
     2641 gaacttataa agtatataaa agaaaacggt gttctagtaa tggcagatat ttcaaattat
     2701 gatgaggcta tcaaagcaca agagtacggt gtagattgcg tttcaactac actatctgga
     2761 tatactccat atactaaaac attagaaggt ccagattttg tattaatgaa aagattagtt
     2821 aaagatttag aaatacctgt aatagctgaa ggaaaggtaa atactcctca agatttaaaa
     2881 aaggtctttg agttgggtgt tcattcatca gtagtaggtt cagcaataac aagaccacaa
     2941 ttaataacag aaaaatttgt taaagcaatt gaaattaatt aaaaattaat ttataaatta
     3001 aaaaattatt atatttaact atatttgaaa attaaaattg gaggtaaaga tatgagaaca
     3061 acagatatga agggaattta ttcagcatta ttagtttcat ttgataagga aggaaatata
     3121 aatgaaaaag ggttaagaca aataattaga cacaatatag atgtttgtaa agtagatggt
     3181 ctttatgtag gaggaagtac aggggaaaac ttcatgcttt caactgatga aaagaaaaga
     3241 atatttgaaa tagctaaaga tgaagtgaaa gaagaaataa aattaatagc tcaagttggt
     3301 tcagttaatt taaaagaagc tgtagaactt gctaaattta caactgattt aggatatgat
     3361 gcaataagtg ctgttacacc tttttactat aaatttgatt ttgaagaaat aaaacactat
     3421 tataatacaa taataaattc tgtagataat agattaataa tatactctat accattttta
     3481 actggtgttg atatgagttt agaccagttt ggagaattat ttgaaaatga aaaaataata
     3541 ggtgttaagt ttacagcagc agatttctac ttattagaga gaatgagaaa aacattccca
     3601 aataaattaa tatttgcagg atttgatgaa atgatgttac cagctacagt tttaggtgta
     3661 gatggggcaa ttggttctac attcaatgta aacggtgtca gagctagaca aatattcgaa
     3721 ttaactaaga atgaaaaaat atcagaagca cttgaagtac aacatgtgac taatgattta
     3781 ataacagata tattaggaaa tggcttatat caaacaatta aattactact tgaagaacaa
     3841 ggtgttgagg ctggatactg tagacaacct atgaaagaag caactgatga aatgaaatca
     3901 agagcaaaag aaatatacag aaagtatttt taataaaata cttttagtat gggaggatta
     3961 gtcaaatggt aggatttact tggatagatt ttgcaatact agtggtttac ttattggcag
     4021 ttttatttgc aggattgtta ttttcaaaaa aggaaatgaa gggaaaagag ttcttcaaag
     4081 gtgatggaac tattccgtgg tgggttacat cagtatcaat atttgccaca ttattaagtc
     4141 caatatcatt tttatcatta gcaggaaatt cttatggtgg tacttggatt ttgtggtttg
     4201 cacaattagg tatgtttata gcaatacctt tgacaataaa atttttctta cctctatata
     4261 gtaggttaga aatagataca gcatatgaat acttagaaat gagatacgaa agtaaaggtc
     4321 ttagagtact tggagctttg atgtttataa tatatcaaat aggacgtatg tctattataa
     4381 tgtatcttcc atcaatagtt ctggctggtc ttacaggaat acctgtaaat gtactgataa
     4441 tagttatggg tgtaatagca ataatttact catatacagg tggattaaag gctgtcttgt
     4501 ggaccgattt tatacaagga atggtattaa tagtaggtgt tacagggaca ttgttctatt
     4561 taatagcaag tataaatggt ggttttggaa cagtaatgga tactcttaca actggacaca
     4621 agttcttagc aagtaatgaa gttatgtttg ataaaaatat actatctaca agtgcattta
     4681 taatatttgt tggagctggt cttaatactt tttcatcata tgtatcgagt caagatatag
     4741 ttcaaaggtt tactacaaca actgatataa agcaacttaa taaaatgaca tatggaaatg
     4801 gtgttttatc aatgggactt gcaacagtat tttatctaat tggaacttgt ttatttatat
     4861 actatactca aaacccagat ttagctcaaa cagttcaaca agaccaagta tttgcatcat
     4921 atatagctta tgaattacca gtgggtataa caggaatact acttgcagcg atatatgcag
     4981 cttctcaatc aactttatcg acagggctta attcagttgc tacaagttgg actttagata
     5041 tacaaactat cataacaaaa gatataagta tggaaaagca aactaagata gcacaatata
     5101 tatcattagg tgtaggtata ctttctatag ctgttgcaat agtattagca aatggagaaa
     5161 taaagtcagc atatgaatgg ttcaatagtt ttatggggct agtactaggt gttttagcag
     5221 gaatatttgt acttggtgca ttttgtaaaa aagcaactaa aatgggagca tatgttggat
     5281 tcatagtatc agcaatttta gttgtttatt taaaataccg tatgccagaa gtaacttctt
     5341 gggcatattc tctaataaca atcacgactt ctgtagtggt aggtacaata gttagtgcaa
     5401 tagaagctaa agttaaagca aaagtatctt taccaaaagc tgagactact gtttattatg
     5461 aaaaataata tttaaatgat aaattgtctt atagagtgat tagataaata atttagttac
     5521 tctataagat tgtataatat actttgtata caaaagtata aaaatagact ttataattat
     5581 atagggagat agtgcttatg atatttggaa atataaatca tagtaagaca tattcaacat
     5641 tgcatgaaga tttacttaga tgttttgaat atgctaaaga taattcatta atagattatg
     5701 aaaaaggtac ttatacaata gatggtgatg atatttttgt gaatattgta gagtataaaa
     5761 cttgtgaaaa agaagataga ttctgggaag ctcataagaa gtatatagat gttcatttga
     5821 tgcttgatgg atgtgaacga atagatataa attttataga aaatttagaa caaaaaatat
     5881 atgaagaaaa gggcgatttc ttgcctcttg atggtaaaaa taatggatac atggaactta
     5941 gaaaaggtga ttttttagtt tgttatccgg aagatgctca tatgacagga ataaaagttt
     6001 tagaaaaaga aaatatcaaa aaagctattt ttaaggtttt ggttagataa tgataattta
     6061 tataaccact agggctgaaa atagtgattt aactttcagc gattagtgag acttcgtaaa
     6121 ttttagttag attactattt tcagccttag ttttttatga gaaaattaaa aataattaaa
     6181 agcttaaaat tttgtaaagg agttaactta tggaaaatat agatgtattc ttattaatag
     6241 gacaaagcaa tgctagagga ttgggaaatc caaaagagag catcattcca aatgaaaatt
     6301 gttttgaata tttgagtact gatgaaatta taaatatgag atgtgagcta gagacttcag
     6361 aaggtgatgg tactatagca cctgcatttt caaatgaatg gaataagctt actggaaata
     6421 aagtttgttt tattcacaat gcaaaagatg gttcaagaat aaaaaactgg aatcatgata
     6481 ataactggtt tctaaatgac actatagaaa aatttaatac aggctgtact accttaagta
     6541 aacattataa tatagaaaat aaatacgtta tttggattca aggtgaaagt gatgcaaaat
     6601 atggtagtga tgtattgtac tataaagaaa gtctaaaaaa aattgcgtat aggcttaaag
     6661 aagaatgtat gattgataaa atgtttgtaa gtttaactgg ttattggctt ggagaagatg
     6721 aatattttat tagaacaaga cgaattgcag cagcacagga gtcggcttgt agtgaatgtg
     6781 atatattatg tgtaggaagc aaaattgcta tgcagtttca tgacagagga ttaactattg
     6841 atgatgttca ttacactcaa gaaggtctta atatgttagg agaggactta tgtaaaaata
     6901 tttataagta tcatataacc aaagaaaaaa gtgtattaag tgatactata gacttatcag
     6961 aagcaaggaa atatatctgt gaattggaaa agataagtaa aagatttgtg taatataaaa
     7021 ttttaaatag agttgttaat ttataaaaat aatttgtatg gagatatgtt atgaaaaatt
     7081 acgtttgtat agatgttgga ggtacttcaa taaaatatgg ttttattaga gaagatggat
     7141 ttataattga taaatcaagt cttgatactg aagcaaagga aaaaggtgga gaaggaatat
     7201 tagccaaaat aaaagatata gtaaaaaaat atatagagga aaatgaaata agtggtatat
     7261 gtatatcaac tgcaggtatg gttgacccag ttgaaggtaa aatactattt gcattagaag
     7321 aattaattcc aaactataag gacatgcaat taaaaaaaga agtagaaaaa gagtttaata
     7381 taaaatgtga agttgaaaat gatgttaatt gtgctggact tggtgaaatg tggcttggag
     7441 caggtagagg tgcaacatct tctatatgtt taacaatagg tactggaatt ggtggatgta
     7501 ttataataaa taataaactg ataaatgggt ttagtaatag tgctggtgaa gtaggatata
     7561 tgaatgtaaa tggaagttct tttcaagaat tggcatctac tagcagtcta attaaaaaag
     7621 tagcaaaaat aaaaaatttg aatgaaaatg acttaaatgg aaaaataatt tttgatttag
     7681 caaagaataa tgatgaagat tgtttaaaag aactcgataa tatgataaaa tcattagcag
     7741 ttggaatcgc aaatatatgt tatgtaataa atccagaagt ggtaatttta ggtggtggga
     7801 ttatggcaca agaaaagttc ctaaaaccaa aaatagacaa agcattaaag gaggttctta
     7861 tagagagagt ttacaaaaac actaatatag aatttgcaaa gagacaaaat gatgcaggaa
     7921 tgcttggagc attatataat tttttaaaca aaatataaag aatagaaggg tttttaatga
     7981 gtattttaga acaattggaa tcaccaactt ttaaagtcac aaaatcagat aaattattaa
     8041 tagcttatat aaaagaaaat atagatgatg tattttataa accaatatca caaatagcta
     8101 aagagagcaa tattggtgaa gcaactataa ctaggtttgt aaaaaaaatg aattttaatg
     8161 gattacagga ttttaaagtt actttagcac aagaaatatc aactatcaat aaaaaaaata
     8221 ttataaataa aaatatacaa aatgatgagc cagctttgga tacagcaaaa aaacttttaa
     8281 gttcaaatgt aaccacttta gagaacacag tagagataat caatagtaag gatgtacatg
     8341 actgcgcaag gcttataata aatgcaaaga aagtttactt cataggtatt ggatattctg
     8401 gtataatagc acaggattca aactataaat ttatgagaat aggtcttaat tgtgtaagtt
     8461 ttgacagtag tcatacaatg ataatgatgt cttccataat ggaagaagga gatttaataa
     8521 ttgcaatttc acattctggt gaaacagagg agattataaa aactgtaaag cttgcaagag
     8581 ccaataatgc taaaattata tcaattactg aaaataaaaa ttctgaatta aaggatattt
     8641 cagatgtaca cttatcttat gtatcgggag aaacagtact ggaaaccggt tcaatatctt
     8701 ctaaattagc acaatttttc attattgatt tagtatatac acaggtagta aaagaacttt
     8761 ctaatgaagc tattgaaagg aaaattaaaa ctacaaatgc tataaaattg tttaaaaatg
     8821 aataaaataa gaaatttgta atgttttata gagctctctt aaaaatttag gaatggagag
     8881 atattacatg tctaaattaa aagattataa tattgataaa gtaataattt ttaataattt
     8941 aagtactaaa acgaagaatc aacttaaaga aaaagttaaa ctaaaaagat tacaaaaaaa
     9001 agaaatttta ttttatgaaa aagatagctt agacagtgtt tatatacttt tagaaggaaa
     9061 ggtatctatt tttaagataa gtgaaaatgg agagagaaaa gtaattttca tattaaatag
     9121 tggggaagtc ataaatgata ttacacttga cgcttctaaa aactctactg ttggctgtga
     9181 agcatttgaa aattcaattg tagcttatta tagtattgaa gaatttttag aaattatgca
     9241 aaatgatttt caacttacaa aggatattat ttcttatatg gaaagaagaa taagacgttt
     9301 atatagacag ttgaaaaatt ctatatctat aaaagtagat aaaaaacttg cagcaaaatt
     9361 atataggtta agtagagaat ttggagtttg ctgtggagaa tggactttac taaatgtaaa
     9421 tattacagtt acatatttag ctgatatgct tggctgtaaa agggagactg tttccagaat
     9481 gataaaggta ttgcaaaaag aagaacttat taaaattgac aataaaggtt tttatgttaa
     9541 agaaactgaa ttatcaaggt attttaaaaa taattaatta tgattaagta agagtattat
     9601 tagaatttta attaaataaa aatattatta agagaatcta tttatagatt cttttttatt
     9661 gtctaattta ttaattttta ttttacccta ttgaattgta taaaaaaaca aataagattc
     9721 ttttttattc aaaaaactta ctaaattttt gattatcttt tcataagttg gtaaatgccg
     9781 atatatcccg tatttgaggg ctttatcggt attttctttt tggaagatac cttgcataag
     9841 ctggcattta ccagcatgaa aatgcaacca aaagtagtaa atgagtagta aatatacgtt
     9901 ccgatattaa caagccagtc tttccagttc tgcttttgca gattggaatg taccgtgtgc
     9961 gtagtagcca agcgtcatgg ttatgttagc gtgtcccatg agatattgca gggtgttcgg
    10021 gttcattccc ttgtttgcca tattcgtaca ataggtatgt cggaatgaat gtggtgtaat
    10081 gtttggtaac ttgtctttgt ggtatttgtt atacttctta atcagtcctc gtaccatacc
    10141 cacatagttt ccagcgactt taggcaatcc ctcacggttt aagaatagga aattgctgta
    10201 accaccaaca accagcggtt gagcttttac tctgttcttt agtattcttt ggagtgcttg
    10261 ataagccctt tccgtcaatg gaatttttcg ttttccgttc tttgtttttg gagtgtcaat
    10321 atagtagccg atttcgctat ctcttaatag ctggtggtct atattgagta ttctgttctg
    10381 catatcaata tgcgttgtca gtccgcaaaa ttcagaaata cgaagtcccg tttccagaag
    10441 aagcacaacc tcatcataat acttgctgta aatcttatcc gtttccataa aggcaagcag
    10501 gcgttcttcc tgctctggcg taagaataac tttcggctcg gtatcatctt ccagaacatc
    10561 acttagctta aattcaaacg ggttctttct gatacagtcg tcttgtatcg ccatataaaa
    10621 tgacgctttc aaggaacgct tatagttgtc aatcgttttg taggcatatc ctctttcact
    10681 cattctgata gcccactctt tagcgtccga ctgtttgacg gtatcaatcg ctctcatacc
    10741 caatgggtca tttttcagag cgttcataag atactgtcgt cctttttccg tagctcgctt
    10801 gatgttcttt ctttggctgt tcttcttgtc gtaaagctgg cagactgtca ttttaccgcc
    10861 tgccgtgtca ataccgtcat caaggtcttt ttttatctgt gcttcttttt ccctcaacga
    10921 tatatcatca cgttttccag caggcgtttt gtctgtcggt acaagtttcc aagcataaat
    10981 aaactgtggc tttccgtata tatcggtata tttataaacg tatcttccgt cttttcgctg
    11041 gctctctcca ttacgcaaat tgcgattttt actgtctttt cgtttcacat tagacatagt
    11101 gtaaagctcc tttccgtcat ggaatgagcc gtgatacgct atacttatta taccatatcc
    11161 acggctctat gacattagat ttcgtccaat gtatctatga ttttttcaaa ttgttttcgc
    11221 tttatctgaa tacggttgcc gttcacgata acccagccag cgtcaatatt ttcttcggct
    11281 aatttacgca acttcttttc tccaatgcgg aagtatttgg acgcttcttc aatcgtcagt
    11341 gtatattttt cccagatagg cacatcagta ttattcataa tttatgcacc ccctttactg
    11401 tgtgtcagtc tataagttat ataaacaggc agacggcttt ggaaagctcc attagtattt
    11461 caactattcc cattcttgtg ggcagaaccg caccatgcgg aagtatcatt atgctgtaag
    11521 ggtaggtcat ggcaaaacga cttttccaac ggtcgctctc ggatcactgc gtgggtcgct
    11581 cgctttcttt tggaaaggtc atggcgtaca gttcccgtgg ctctctatcc ttacaaacgt
    11641 atctgtctgc tttattcagt tttcaaagag ctgtggcgaa agcggtatgc tttcatgtaa
    11701 aagcaggagc agagcaggaa agaggggatt gaacaaatca tgctctgcgt tactgctggt
    11761 tattcttctt caatttctaa ggcaatatca aaccctaaaa tcattttgat aagtgcttct
    11821 cggataagtc cttttaactc catatctaca acgatataaa cattaccgta ttcatcatag
    11881 agtggacgca aacagcactt cgatatgtaa gggtcataaa atgcaagtac cttttcaatc
    11941 gctttttcgt ccccgtccat agcttgtgaa agcaagtagt aagacggctt cttaaatgtg
    12001 tgtttcattt gctatcgttt ctcctttaat agttttctca taagtgccaa tgaattgtgg
    12061 cggttctgaa aaactccgct tctggaaatg ttttgctttt tggcgatttc ttcattgttc
    12121 atttccaaaa agtaatacat caacaaagtt tctcggttac gttcggataa ctgtttcaag
    12181 gcttctgcca attcatcatc atagacacga atatcattac cgcatacatt gaaaatcgta
    12241 tagtctgttt cgtagtcgtc ccatacagcc aagttttcaa ccatgatttc tggaagttca
    12301 caaaaggaaa tttctttttc ttttcttcgc ttcaatccct gctgatagtc cttgacaaca
    12361 tgacggacaa ctttcttcaa acagctttca aaacggcatt gaacagtttt ctggaagtca
    12421 gacggtttca tcaatctcac cccctttccg ttatgacgtg aaaagtgttt atccctcttt
    12481 ccgcaccata tagggaatgg caagtgtgat ttgttaagtt gaaaccagaa aaaatcgaaa
    12541 aaacttttta gtaaaacaaa aacagcccgt gtgaatgtga atacacaaac ggactgcttt
    12601 ataaatgcta tctgtatgaa tttttaactc ttgtagaggg tagaatgatt gtaatactga
    12661 actaaaatat tttttcagtt gggaacagca aagtattatt ccatatacaa tcatggcgta
    12721 gcccccttta ccagagctat accttgtgcc accaatgaaa aaggacggct ctggctatat
    12781 ccaaaaccgc cccgttatgt tttcaagacg cacaaaagta tatcatctcg gaacggttat
    12841 ggtgatttaa aatgagaaaa acaaaagaaa cacatacttt tgatttcaga ccgctagggc
    12901 tggcaatcag agaagcccgt gaaaaagctg ggctttctcg taatgattta ggcgataagg
    12961 tattctatgg ggaa
//
LOCUS       DAMKMI010000114        12824 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMKMI010000114 DAMKMI010000000
VERSION     DAMKMI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.118"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            177..845
                     /locus_tag="PC429_002850"
     CDS             177..845
                     /locus_tag="PC429_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848339.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            847..1332
                     /locus_tag="PC429_002851"
     CDS             847..1332
                     /locus_tag="PC429_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF3848340.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1393..2514
                     /locus_tag="PC429_002852"
     CDS             1393..2514
                     /locus_tag="PC429_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF3848341.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2795..3985
                     /locus_tag="PC429_002853"
     CDS             2795..3985
                     /locus_tag="PC429_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3848342.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4119..4374
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4433..5434
                     /locus_tag="PC429_002854"
     CDS             4433..5434
                     /locus_tag="PC429_002854"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF3848343.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5538..5927
                     /locus_tag="PC429_002855"
     CDS             5538..5927
                     /locus_tag="PC429_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF3848344.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6088..7296
                     /locus_tag="PC429_002856"
     CDS             6088..7296
                     /locus_tag="PC429_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF3848345.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7382..8458)
                     /locus_tag="PC429_002857"
     CDS             complement(7382..8458)
                     /locus_tag="PC429_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3848346.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8687..9214
                     /gene="hpt"
                     /locus_tag="PC429_002858"
     CDS             8687..9214
                     /gene="hpt"
                     /locus_tag="PC429_002858"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF3848347.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9303..10235
                     /locus_tag="PC429_002859"
     CDS             9303..10235
                     /locus_tag="PC429_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF3848348.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10297..11523
                     /locus_tag="PC429_002860"
     CDS             10297..11523
                     /locus_tag="PC429_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3848349.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11607..11828
                     /locus_tag="PC429_002861"
     CDS             11607..11828
                     /locus_tag="PC429_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF3848350.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            12017..12187
                     /locus_tag="PC429_002862"
     CDS             12017..12187
                     /locus_tag="PC429_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848351.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12280..12741)
                     /locus_tag="PC429_002863"
     CDS             complement(12280..12741)
                     /locus_tag="PC429_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848352.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
BASE COUNT         4965 a         1343 c         2262 g         4254 t
ORIGIN      
        1 aaaaaatatt atatttttaa acttgaaaag taagtaatta taagctattt aatcactgga
       61 ataaatttac aataaaaaaa taaatacaaa ggctttatca ttaaggtatt ggcataagtt
      121 ttgcttatta aacaagtata ctaataaaat tcatttaaac aggagggaga atttatatga
      181 gtaaattaga atcaacaaca gaaaaagttg aggtaaaatg gtatgggtat gtagcattaa
      241 tattgggagc attatttttt tcaggaatat ttaaagatgc accaggacca ttaaaagtat
      301 tggattttaa caatgtatta ggttcttttg gaacattggg aacaattaat gatggtgtag
      361 gtacattagc tgcaaacttt agaggtgatg gaggtactgg ccctcgagat ggatggctat
      421 atgcattgac attaatacca tcagttatgt ttgcacttgg agtagttaga gttatagacc
      481 acttagatgg aatgaaggca gctcaaaagc ttcttagtcc actattaaag ccattattag
      541 ggttacctgg atttgcagga ctaactttga tagctagttt acagtctaca gatgcagctg
      601 catctatgac taaagaactt aaagatgatg ggtatataga tgaaaaacaa aaagctgtat
      661 tttgtgcatt tcaattttca ggtgcgtctg caattacgaa tttctttgct tctggagcag
      721 cactatttcc atttatagga gatgtaccaa tatttatacc acttgcttta atattaatta
      781 tgaagtttgt tggggcgaac ttacttagat tatatttaaa taaatttgac aagggggaag
      841 cttaacatga gtgaggtagc aaaaaaagaa gtaaaagtta aaaaaggaaa tgtgatagat
      901 gcgtttattg gaggagctag aaatggattc caaataagta caaattcaat ggctccaaat
      961 gtcatcttcg gttttgctat aatttctgtg ttcaatttaa caggtctttt ggatgtaata
     1021 gggacaatat ttacacctgt aatgagtatc tttggacttc caggtgttgc agcaacagct
     1081 ataatgacaa tatttatatc aatgggagga gcatctggag taatcgcagg tttatttaca
     1141 gcaggtcaaa taagtcaagc acatgttgca attctattgc caatgttatt gctttcagga
     1201 gcgcagatac aatttatggg gagactttta ggaacagctg agttaaaaac taaatattat
     1261 ccacatatgt ttgttatagc aactttaaat ggttgtattg caatgcttat aatgaattat
     1321 ttcgtagtat aggtaattat aataaagatt taatataaat ggaaagttta aattttaagg
     1381 aggagctaga ttatggaaac taacaaagac tatattgagt atgtacataa agtgtatgat
     1441 tatctacaca ctataccaga aaaaggattt gaagaatata agacatcaga gtttgtttta
     1501 aaagagcttg aaaaaatggg gtttgaaaat attaataaag gaattgcagg aactggtatt
     1561 atagtaacta tagattctaa aaaagaagga ccagtatttg ctataagagc agatatggat
     1621 gcattggaat ttatcataga tggagaaaag aaaatgatac atgcttgtgg tcatgatggt
     1681 catacttcta tgcttttaac agctgcaaag ttaataaaag agagaaattt agtaaaaaga
     1741 ggtaagttaa aaatactttt tcaaccaggt gaagaaaaat tatatggagc attaagagta
     1801 attgattctg gtttgttgga tgatgtagaa gaaatggtag ggatgcattt aagacctgga
     1861 caagattgta ggttgggaaa ggcgatacct gcaatgtacc attgtgctag ctgtataatg
     1921 gaatttacga ttaaaggtat cacttgtcat ggttctaaac cacatttagg agtaaatgca
     1981 gtagatgctg tctgtttagc agtgaatgca ataaatgcaa taagagtaaa tccagctata
     2041 ccaaattcta taaaagtaac aaatatatca gttaagggag aaacttataa taatatacca
     2101 gatgaagcat ttatggcttt agatataagg gcagaaagta atgatataat agaagaatta
     2161 aaagaaaaag caatacatgc tgtaaaaact gctgtaagtt ctataaatgc agaagctgta
     2221 ctaatttcca aagatggagt accagcagca gaatttgatg aagaacttgt aaacttggct
     2281 gaagaaacta taaaagatgt attgggagct gatgcatcta taggtatatt taaaaattct
     2341 ggtggagaag atttccatta ttatacacaa aaactaaaat gtaaaactac atatattgga
     2401 ttaggagctg atgctacacc aggatttcat aatccaaatg taacatttga tacgaaagct
     2461 ttagaatatg gtgtagatat atggtgtaga ttggttgaaa agagattggg ataacatcca
     2521 aactaataat tctacattta aaattataat tatagaagtt atagaaaaag attgcttatg
     2581 ttaattagaa aataattaat atgagcaatc ttattttgat ataatgaaac tgattttgga
     2641 atattgaaaa aatatgatgg aaaagtaatt atattttaaa tctattggac aaaaaagtga
     2701 tattattata atattaagaa ttgtaaaata ataccaaagt gcatctagta atagacttaa
     2761 caattataaa aattttattc ttaggaggga aaaaatgagt aagtataatt ttgacagagt
     2821 aatagataga gtaggaacag attgtgtaaa atgggatttt agaactaatt gttcaacaaa
     2881 agcacaaaaa gatggtttgc ctttttggat tgctgatatg gattttgaat gtgcagaacc
     2941 aataatagaa gcattacgta aaaaagttga acataaaata tttggatata gttcaaatga
     3001 tagtgataaa tactttaatg cagtttgtga ttggtataaa aggagattta actgggaaat
     3061 aaatagaaaa gatattattt ttagtcctgg tgttgtacct gctgttgcta ttttagtaag
     3121 aatattaact aattctaatg aaggtgtaat agtccaaaaa ccagtgtatt acccatttga
     3181 agctaagata aagagcaata atagggatgt tgtaaacaat cctctgatat atgagaatgg
     3241 tacttatagg atggattatg atgatttaga agaaaaagct aagtgtagca acaataaagt
     3301 actgatactt tgtagtcctc acaaccctgt tggaagagtt tggagagaag atgaattaaa
     3361 aaaggttgta gaaatatgta aaaaatatga cctatggata atagctgatg agatacattc
     3421 tgatttaatt agaaaaggat ttaaacatac tcctttacag tcattatgcc cagagtacaa
     3481 ggataaaata gttacatgta tagctccaag taaaacattt aatctggcag gaatgcagtt
     3541 gtctaatata atagtgaata atgatgaact aaaaaagaaa tatcaagaag aggttacagc
     3601 tgttggagtt gcaacttcac caaatccctt tgctattgta gctacaatag cagcatataa
     3661 tgaaagtgaa gactggctta atgaattaaa tgattactta gataataata tacaatttat
     3721 tgatgaatac ttaaaagaaa acctaccaaa agtaaaactt gtttatccag aaggaacata
     3781 tctagcatgg ttagatttta ctgcttatgg attaaatgaa gtagaacttg aagatttaat
     3841 gtttaaaaaa gctaatgtat tatttgatga aggttatata tttggaaaag aaggaatagg
     3901 atttgaaaga ataaatgtag cttgtccaca gtctttatta aaagaatgta tggacagatt
     3961 aaagactata tttgaaaatt tataaagtat aggtaaaaat ttataaagtg tttaaaaata
     4021 aaaaaataat aaacttgaca aattttaact aatcacttat catactatta tgaataaata
     4081 gaaaacataa gagagctaaa ttttattgaa ttaaactctt aaagactttg aaaggaagag
     4141 tagttataga atgtaattaa agcgagttgg tgatggtgtg agaccaatat gaagtctata
     4201 atgaagaaca tcctggagtt gctaaccgaa attttaaaag agtaggctta gtcggaacca
     4261 aaccgttatc atattggggt gtatcgagta atatctgtac attgtgagct gggtttgaat
     4321 ttatttcaaa ctaatcaggg tggtaccgcg gaaaagcaac ctttcgtccc ttttatatag
     4381 ggatgaaggg tttttttaat ggaaaaatat ataaatatag ggagtgaatg atatgagcac
     4441 aataatacca aaggattata aaagtagttt gaacgtaatt gatacacaaa tagctataaa
     4501 aaaattaaaa gatttttttg aaatgagact aagtaatgaa ttaaatctta taagagtttc
     4561 ttctccgtta tttgtattgc cagaaacagg tgcaaatgat aatttaaatg gagaaaaggc
     4621 tgtaagcttt gatataccat ttatgaataa aaatgcagaa attgttcaat cacttgcaaa
     4681 atggaaaaga ttagccctta aaaaatatgg gtttgaagtt ggtagtggtt tatatacaga
     4741 tatgaatgca ataagaaaaa atgaagaatt agacaatata cattctcttt atgtagacca
     4801 atgggattgg gaattggtta tagataaaag tagtagaaat gaaaagactc taaaagatgt
     4861 tgtaaaaaga ttgtatggag tatttaaaga tactgaaatt tttgtatgta gtatgtatga
     4921 aggtataaaa gaaatattac cagaagaaat aacttttata acatcacaag agttagaaga
     4981 tatgtatcca gaattatcac caaaggaaag agaagataaa attgtaaaag agaagaaagc
     5041 agtatttata actcaaatag gaaaaacatt aatatcagga gaaaaacatg atggaagaag
     5101 ccctgattat gatgattggg aattgaatgg ggatatatta ttttacaatc ctgttttaga
     5161 ttcagcatta gaattatctt caatgggaat tagagttgat gaagaatctc ttgatagaca
     5221 attgaaactt gctggatgtg aagagagaaa agagtttgac tatcataaga tgcttttaaa
     5281 tggcgaactt ccgtatacaa ttggtggagg aattggtcag tcaagaatat gtatgtattt
     5341 cttacaaaaa gctcatatag gagaagtaca agttggagta tggccacagg atatgattca
     5401 aaattgtagt agtgcaggaa ttgaattatt ataatttaaa tataaactaa ttgataaaat
     5461 ttatatctaa tatgttcatc aaattaatta tatcggttat aatgtagaat aacctataat
     5521 tataagaggt gataaatatg gagtttttaa aaataatcat taatatagtt ttagatattt
     5581 taaaaaaaat tttggtcaga tttaaaaatg ctaaatttgg tttggttttt gtatttgatt
     5641 tacttaaact acctgatttt atgacagata aaagaatcaa tatagttgat aaaataaagg
     5701 taatttctgt attaattttt actatatcct actttgtatc tggggttgat ataattccag
     5761 agatgattgc aggggcattt ggttttatag atgatgctat agttttaatt tggagtatag
     5821 gtatagtgaa tgaagaaatt aataagtata gagttatcgc aaaaaaagat aaacattcaa
     5881 atataattga aaatgtagaa ttttctataa aagatgaaga agaataacaa tttatatatg
     5941 ttattctttt ttttattaac aaaaaatggt taaattgttg acggagaggt aaaactctat
     6001 tatcataata agtggatatc caatatttaa ctaagtattc aatgtgcaag aatgcttaat
     6061 ttaaaaaagt tatggagggt aaatattatg aagaaaaaaa taggtttagt gcccaaacta
     6121 atcatcggta tcatagtcgg tattttaatc ggaagttatg caccagaaat aatagtacaa
     6181 atattagtaa cagttagtac tttattctca gcattcctaa aatttgttat accatttata
     6241 attataggtt ttgtaacagc aggtatagct gatttagcaa caggtgcagg aaagttattg
     6301 gggataacta cgggaatagc atatggttct accttaatag caggattatt atcatttgca
     6361 gtatctacat taatcttccc aaattttatt gatgcaagtg ttgcaagtca aattggagac
     6421 ccagaggcag gaatgttagc tccaatattc acaattccat tagagcctat ggtagatgta
     6481 acagctgcaa tcgtatttgc atttgtaatg ggacttggaa tatcagcttt aagaaatcat
     6541 ggaaaaggag agactttatt taatttcttc caagaatttc aaggaatcgt aacgaaaacg
     6601 ttgtcaacag tgattatacc tttattacct ttatacatag caggaacttt tgcaaatata
     6661 actatagcag gggaagtatg gacaatatta ggggtattct ggaaagtata cttagtagta
     6721 atacctttac attttgtata tatggcatgt caattcacag cagcaggtgt attttcagga
     6781 aaaaatccag taagaatgtt aaaaaatcaa gtacctggat atttaacagc tataggaact
     6841 caatcttcag cagcaactat accagttaat gttggttgtg cagaaaaaaa tggagtaagt
     6901 aagcaaataa gagaatttgt tgtacctctt tgtgcaacaa tacatttatc aggtagtatg
     6961 atatcaataa caggatttac aactgcagtt ttaatgatga atggtatgcc acatggaata
     7021 ggtgtattat tcccttatat tgcaatgtta ggtatagcaa tggttgcagc accaggagca
     7081 ccaggtgggg ctataatgag tgcacttcct ttcctaccaa tggttggaat accatcagat
     7141 ggtgggcttg caagtttaat gatagcttta tacttaactc aagacagttt tggtacagca
     7201 tgtaatgtat caggagataa tgcaatagca gtagtagtag ataaaataaa tgatggaatg
     7261 agtaaaaaag gaaataaaga agagcaaagt gcttagtttt ttaataatca aaaatgagtt
     7321 atctcaaata gagatagctc attttttatg gtataaaaaa ttataaaata aagtgatttt
     7381 tttatttaaa aattgcttta agagatgata ttaataattt attttcatta ggttttaata
     7441 tacatactct aaagaaatac tcatctagac cttcaaatga acaacaatct cttattatta
     7501 ttttttgtgg aagcaattgt tctcgaagag atttagcagt caattccttt gtcttgattt
     7561 tacacaatat gaaattgcct ttagtattgt atatatctaa gctttttata ttcttaagct
     7621 ctttaagcag gtaatctctt tcagtgttca ttaaggaaat agtatttgat ataaaatcta
     7681 aatcactaaa catgatttca cccattttag aagctattat gtttatattc cataaatcta
     7741 gatttttatt tatttcattt ttgacattag tgttagatat aagaccatat ccaagcctta
     7801 ttccaggcgt tgaaaaaaat tttgatgtac ctcttataac gaataaatta gaataatcat
     7861 caactaattg agtacaagag tatgtatctg tatcagtaaa ctctacataa gtctcatcaa
     7921 tcattaaaaa tgagtctgta ttttttaaaa tttctctcac ttctacttta gtaaatgcaa
     7981 atccagttgg attactagga ttacagatta ctaccaaatc ataatcttta gcattgatag
     8041 tttttataag attttctaaa tttatatgaa aattatcttc ttcttttgca aagtattttt
     8101 ctatagaaca attaatttta gataactctt tttcatactc tgaataggct ggagagagta
     8161 aaagagcctg tttagggtta attgtatgta taaatgatga aattagttct gtagctccac
     8221 tgccaagtac tatattatct atagaacatt tgcagtaatt ggatatagaa gtttttaagt
     8281 ctatgtaatc agggtcagga tacatagata ccatatctat attgtttaca atgtattgtt
     8341 tggctaaact tgatgtacca aaaggattta tatttgagct aaaatccata aattcttcct
     8401 ttgaaaagcc atacttacta gataaactat ataaattagc tccatggttt gtgctcataa
     8461 aagtacctcc aaaaataaaa ttatataatc gataaaaata actgattaat atatgtacat
     8521 atagattata atacaaagga atcgttattc caaattttta atggaaaaag caaaaaagta
     8581 tggataagct gttaatttag gtttaaaaat gatgaaaaag attttaattg atattttggt
     8641 atgctataat atataagtta atagaaggaa agcgggagga tacaaaatgt acaaagttac
     8701 aggaaaaatg ttgacagaag aacaaataaa agaaaaagtt tatgaattag gaaaaaagat
     8761 agaagaagac tttaaaggcg aagatttact agtggtaggt atacttaaag gagctagtgt
     8821 atttgtttca gatttaataa gatgtataga tttggatgta aatatagatt ttatgagtgt
     8881 tacaagttat ggaaactcaa cagagtcttc tggaacagtt aaaatattaa aagatttaga
     8941 tgtagatatt gaaggaaaga atgtgttgat tgtagaggat ataatagatt ctggtttaac
     9001 attaagtaat ctagttgcag ctttaaaaac tagaaatcca aaatctttaa aactatgtac
     9061 tctacttgac aaacctcaga gaagaaaagc taatatacct gtagactatg ttggatttgt
     9121 tatagaagac aaatttattg ttggttatgg aatagactat gctgaaaagt atagaaattt
     9181 accttatata ggtatagttg aggatgtaga atagatttaa atgtgaatca aaagcactaa
     9241 tttctaaggg agttagtgct ttttagttaa ttatataaaa ttttagtaaa ggagacatct
     9301 atatggatat tcagttaaat atattattat ttttccaaaa tttaagaaat ccaattttga
     9361 actttatatt tcttgtcttt acaataagca cagaagcacc gttattaata ttaattacaa
     9421 caattatata ctggtgtata aataaaaaat gtggacagaa aatactgttt gctataattg
     9481 gaaattttgt tgtaaattta ggcatcaaag agtttgtaaa agctcccaga ccaataggaa
     9541 taaaaggtct tgaatcattg agagtttcta ctgctggtgg ttattctttt ccaagtgctc
     9601 atactcaaac tgcaacaaca ttttgggtta gtattatgac tatatttaaa aagagatatc
     9661 ttcatttaat aggtgcagtt atggttttag gagtaggatt gtctaggctg tatttagctg
     9721 ttcattggcc aatagatgtg atagcgggtt ggatattagg aatatttttt acagttgtat
     9781 ttgtaaaaat atttgactat attgatgata ataaaaatta tattttactt gtagttttac
     9841 ttataccatt tataatagtg gctatatttc taaatagccc tgaatatttt gaaaaatttg
     9901 gtataatagt aggatttgta tttggatata tggttgagga tagatttgtt aaatttaata
     9961 cagataataa taacaaaaaa ataaatttta gtaataaaaa taaggggagt aaaaatataa
    10021 ttttttcaag aatatgtagg tttttggttg ggatagtaac aataggaatg ttatatattg
    10081 gcattaagtt gtttatttca atgttaatag tcactttaaa tataagtgac tctactatga
    10141 gtataatttt tatgaatttt attaagaata cagtagttgt tttttatgga atagcaggag
    10201 ctccagcatt atttaaactt ttaaaattaa attgatttta aggtatagag tcaatagttt
    10261 aaattaaaaa aattaagggt agaaaagagt gattatttga agaaggtagt aataataggt
    10321 ggtggaccag cagggatgat agccgcatca actgcatgtg agaaaggcta tgatgtaaca
    10381 ttaattgaga aaaatcataa attaggaaaa aaacttgcaa taacaggaaa aggtagatgt
    10441 aatattacta acgcttgtga aatagaggaa ttaatagaaa atgtacctac aaatggaaaa
    10501 tttctttata gtgcttttta tacttttaca aatgatgatg taataagtat gtttaataat
    10561 cttggagtaa agactaaaac agaaagagga aaaagggttt ttccagagag tgataaagca
    10621 tttgatattg taaatgctct tgaaagacaa cttaaaagta agaaagtaaa tatattatta
    10681 aattcgaaag tagaaaaaat aatatctaaa aataataaaa ttgaaaaagt aattttaaac
    10741 gataaaaaag aaattaagtg tgattcagtt gtagtagcaa ctggtggatt aagttatcca
    10801 cttactggtt ctactggtga tggatataaa tttgcaattt cacaggggca cacaattata
    10861 gatacaaaac catctttaat tggtatagaa gtacaagaaa gttttacaaa agatttagaa
    10921 aaattatcat tgagaaatgt tgaaataagg gtgtttaata gcaaacaaaa gaaagtttat
    10981 agtgattttg gagaacttga atttactaaa tttggtttag atggacctat aatcaagtct
    11041 gcaagttgta gaatgaaaga tacaaggaaa gaaaattaca ctatattatt agatttaaaa
    11101 cctgcattag atgaggaaaa attggataaa agagttcaaa aagattttca gaagtataca
    11161 aataaaaaat ttgaaaaagc attagatgat ttacttccaa aaaaactaat acctataata
    11221 ataaacttaa gtgaaattaa tccaaatacg gttgttcacc aaatttctag agagcagaga
    11281 aaaaacttag tccatttatt aaaaaattta aagtttacag taaagagata cagacctatt
    11341 gaagaagcta taataacttc tggtggtgta aaagtaaacg aaataaattc aagtacaatg
    11401 gaatcaaaat tagtagaagg tttatttttt gctggagaag taatagatat agatgcatat
    11461 acaggtggat tcaatttgca aatagctttt tctactggat atttggcagg ttttaattgc
    11521 taaaaattac ctgaattaaa aatgtttaaa atcacataat attatcagca acacaaaata
    11581 aattaaacag aaggaaggta atcaacatgg caagtaacaa taacaacaac agaacagtag
    11641 taccaggagc aaaagaggct ttaaaccaaa tgaaattaga aatagctaat gaacttggta
    11701 tgagtaatta tcaacaagtt gataaaggaa accttactgc aagagaaaat ggttatgtag
    11761 gtggatacat gactaaaaaa ttagttgaaa tggctgaaca acaaatggct ggaaaaagca
    11821 acagatagtt ataaataatt atttataact attggctatt agtttagcta gagctatctc
    11881 aaatatagaa agtaatttct aaattgagat agcttttttt tattaaatta ttaaatagat
    11941 atatttttat gtaataaatt ttatgtattt ggataaaata atacaaatga atatgttaca
    12001 taaggagtga tgtaaaatga gtagtgtaaa ttttgaggat ttaaaaaaca aatttattaa
    12061 tagtgactta gatgaaaaaa taagaatata tacaacaaca gaaggtttat cagtagaaca
    12121 atttaaagag ctactaaaat attatccaat acaacattta tctaaactag aaaaagcact
    12181 tggatagcag tatttaatcc aaaattaaaa aggcaattta tttaatatta aaaatatttt
    12241 ttattaaata aattgccttt ttataatagg tttaaattat tatgcttttg atatataatt
    12301 atagattctc tcatttccaa gttcaataga cccagtatta ataagagtaa ctattccttc
    12361 tatatctcta gcagttaagt atttatatgg aattaataaa ttataaccag tattacttag
    12421 taagtttttt aattgggtta tttgagagat attgaaacat tttgcagttt tagtagattt
    12481 ttttcttaag ttagataaat tttcattttg aatatttatt aaagtttcta tatagtttct
    12541 ataagatatc ttaaagtttg tctcattgac tattttttca ttaagtatac tactgataaa
    12601 atcttgagac aatatattat ctgcaaattt atctgctgtt aattttccag atgttatgcc
    12661 tttttgatat tttctaagaa tttttttagt atagcagtat attttatttc tcgtttctaa
    12721 atcgagttgt tctatttgca caaaaccact tccttctaat tataaaaata tttattaaaa
    12781 atctatactt aactaataaa gcttcaataa cttatttaca ataa
//
LOCUS       DAMKMI010000115        12590 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMKMI010000115 DAMKMI010000000
VERSION     DAMKMI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.119"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            144..464
                     /locus_tag="PC429_002864"
     CDS             144..464
                     /locus_tag="PC429_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF3848353.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            602..1246
                     /gene="trmB"
                     /locus_tag="PC429_002865"
     CDS             602..1246
                     /gene="trmB"
                     /locus_tag="PC429_002865"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF3848354.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            1375..2121
                     /locus_tag="PC429_002866"
     CDS             1375..2121
                     /locus_tag="PC429_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848355.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(2250..2528)
                     /locus_tag="PC429_002867"
     CDS             complement(2250..2528)
                     /locus_tag="PC429_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848356.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            2803..3249
                     /locus_tag="PC429_002868"
     CDS             2803..3249
                     /locus_tag="PC429_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848357.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            3519..3971
                     /gene="accB"
                     /locus_tag="PC429_002869"
     CDS             3519..3971
                     /gene="accB"
                     /locus_tag="PC429_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF3848358.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            3987..5348
                     /gene="accC"
                     /locus_tag="PC429_002870"
     CDS             3987..5348
                     /gene="accC"
                     /locus_tag="PC429_002870"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF3848359.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            5363..6217
                     /locus_tag="PC429_002871"
     CDS             5363..6217
                     /locus_tag="PC429_002871"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF3848360.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKLLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            6238..7182
                     /locus_tag="PC429_002872"
     CDS             6238..7182
                     /locus_tag="PC429_002872"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF3848361.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            7371..7631
                     /locus_tag="PC429_002873"
     CDS             7371..7631
                     /locus_tag="PC429_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF3848362.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            7784..8155
                     /locus_tag="PC429_002874"
     CDS             7784..8155
                     /locus_tag="PC429_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF3848363.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            8185..9030
                     /locus_tag="PC429_002875"
     CDS             8185..9030
                     /locus_tag="PC429_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848364.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            9351..10478
                     /locus_tag="PC429_002876"
     CDS             9351..10478
                     /locus_tag="PC429_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3848365.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            10582..11217
                     /gene="lexA"
                     /locus_tag="PC429_002877"
     CDS             10582..11217
                     /gene="lexA"
                     /locus_tag="PC429_002877"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF3848366.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(11364..12521)
                     /locus_tag="PC429_002878"
     CDS             complement(11364..12521)
                     /locus_tag="PC429_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3848367.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTLDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
BASE COUNT         4832 a         1434 c         2022 g         4302 t
ORIGIN      
        1 aaatattata taaaatattt taaagcctta ggtaatatta ggtagaagtt ttagtaaaat
       61 atgcaaaaac taattgatta tgtaccttgt atgggtatat aatagatatt gttttaataa
      121 aatatttgaa agggaatgat ttgatgaaaa ttactttacc agaaacagct attgatactt
      181 taaaaagtat cttaaaagac aatcaagata aacctaataa tataagagta tactttgcag
      241 gtgttggttg tggaggtcct tcttttggat tagctttaga tgagaaaaaa gaagatgact
      301 taacttatga agttggagaa ttacaatttg taatgagctc tgatgaatat agtcagtatg
      361 gagatataat aatagaagat acaggatttg gatttagagt tattcctgaa aatatgaaag
      421 atcaaggtgg aggaggatgc tctggttgtt cagggtgcca ctaagattaa gtttttagta
      481 agtttagaac tctctttata gaaataaatt tctattttaa gagagttctt tttatttttt
      541 ggtgagataa tgtataataa aaatgtattt tactaatttt ttatatgtaa ggagaattat
      601 tgtgagaaga agacgaaaaa aaggtgcaga tgaaaagctt ttaagttata caaaatatgt
      661 tttaagagac gatatagaca aattaaaagg caagtggaat ttaaagttta gaaatgataa
      721 tccaatacat gttgaatttg ggactggaaa aggtaagttt ataacaactt tagctaagca
      781 gaaccctgac ataaattata ttgccatgga acttaaggaa gaagttcttt taaaggctgt
      841 tgaaaaagca gatgcttcta atttgaataa tatactattt ttatggggag atgtaagcaa
      901 tattttggat tactttgaag ctaaagaatt atcgagaatt tatataaatt tctgtgaccc
      961 atggcctaaa aatagatgga gtaaaagaag actcactcat tctggatttt tagaaatgta
     1021 caatagggtt ttggaagatg atggcgaaat tcactttaaa acagataatg aaaaattatt
     1081 cgagtttagt ttaaatgaaa ttgctgcaaa taattggcta ttaaagaata tttctcttga
     1141 tttaggaaat agtgagtatg aaaataatgt tacaacagag tatgaagaca aatttatgtc
     1201 acaaggtatg cgaatattta gatgtgaagc taaaaaacgt aactagtaat atgtttataa
     1261 gtttaatagt agaaatttat tttaatttat attaataaag tgtacgaaag taggtatata
     1321 taacaaggaa acagaaccct ctcatagaaa ttaaattatt ataataaaaa atttatgtgg
     1381 gagggtacta tgaatttagc taatttaata ccatttatta tttctgcagc agctttaagt
     1441 ccaataaatt caaatgataa ggttttgagc gaaaatcaac ttaaagatat ttttcctgat
     1501 gcaaatttaa gagcagttgt aaaaagatat ataaatccag atgagatgac tataagcaat
     1561 ataaaggctt tagatggaga attttatgca actggtgaga gcatttcaaa tttaaaaggt
     1621 atatcatatc ttgaaaatgt agacaatttt attttttgga ataataatat aaaagagatt
     1681 cctaaggaag ctttaagtct aaaagatatg gattcaataa atcttgccaa taattatttg
     1741 attgatgacg atgtagtaaa ttctctttct cataatggtg tggatgtaaa ttgcgattta
     1801 aattttattg atacaaaaga taatcagtat aaactggact ctaaatatca taacgttgat
     1861 atagcaaaag gtgaaagtat tgatttgaga aaaataatta cgaagaaaat tgatagttat
     1921 tataaatact gggaagttac tgataacctt ccaaaagatt taaattttat tgtatctgta
     1981 agtgataaga gtgtactttc atgtgaagat atgataatta aaggtaataa aacaggtcaa
     2041 gcagtggtaa aagttatact aagcgacaaa aatgatttaa atacatctca agaagttcta
     2101 attactgtca atgtaaaata atctgtcaac ttattcaatc attctatttt caatttaagt
     2161 gtataatcaa aataaaggtt gttagtaaca gaacgctaat attagtgctt tgttactagc
     2221 aacctttaaa ttttatagaa atagattatc taaaaaattc catttcttct gttagtagcc
     2281 catattccta ttaaaattat cagtgaatat attatataca aaactattct tatatattgg
     2341 acatctaatc caaaaaattc aatgttactc tgtgaggtat gcaacatcac aaggactgca
     2401 gatataccaa taagcaaagg attgattact atttgcttat cagttcttaa aatcattatt
     2461 ttacccatcc aaattattga taatataaaa aataaaattt taagaccaaa ttctaatgta
     2521 tctaccaaag cgaaaacatc ccttcattta taataaattt ttcagctcaa caaaatataa
     2581 aaccctacaa acaacttttg aatccataat aagataactt gattaagcac tatgaaataa
     2641 gtactataaa acttcatcta cattaatata tactacacta tacttcaaat ttcttaaaca
     2701 taaattaatg aaattaacaa aattttattc agaaacatat aaagatatat tttactattt
     2761 aaatgcatat cattatatat acagttttag gaggtgaggt atatggaatc taaatttttt
     2821 aattggtaca ctcaatcctt agggggaact tttggcatta tatcatgtat atgcgcatat
     2881 ttaaatggat acatgtttgt ttatacaaat agtgctagca aatattttga aagtatagga
     2941 tttctgggaa taatttcaag ttatacacta cttccacttt gtatgcttac tttgattttt
     3001 ggagttatat attcttatac tccagaaaca tccgacaaaa gtatttttaa tatacctttt
     3061 tttactttaa ataaaagatt aatacttatt acagtagttt ttggttttat aggtgctaga
     3121 gtttacttta taattcctgc aattctaata acttttaacc tatatagcaa tgttttgata
     3181 gctaaattaa agtacatcct atgtaaaaat aaagaaaatg atactaattt gaaagaaagc
     3241 gaagtctaaa ttgcataata ttggtatttg tcttaaaaat tcccgaggta ttaatttgta
     3301 ttctcttgta ttttgcaaaa aatattgaat tttattcatt ttatacttat aatttatatt
     3361 aataatggaa aattaaaaca aatataagtt actggttgtt aatttaaaat taaaagatat
     3421 attgatgtgt tgattctcta gtcagcactt tttcttttat aaatttgttt tttgtgatat
     3481 aatattagca ccaggtaata atacaagggg gtaacaaaat gaacatcaat gaaattaagg
     3541 aattattaaa ggccatagac tcaactaact tagaatatgt aaaacttgaa agtagcgatt
     3601 taagattaga agtatctaaa aaagctcaat ctacaagttc acctgtttta agtgtacaac
     3661 aagaatcagt agtggatctt tctttagaaa agccagttgt aaacgataca cctgtcacat
     3721 ctaatgagaa tctatcagta gtagtagctc ctttgatggg aacattctat gattctccaa
     3781 gtccagatgc agatagcttt gttaaagttg gcgatgtagt tgaagaaggc gacactttat
     3841 gtatattaga agcaatgaag cttatgaatg agataacaag tgaaattaaa ggtgaaataa
     3901 ttgaagtatt agttagtaat gaagaactag tagaatataa tcaaccttta tttaagataa
     3961 aaccattgta gtaggaggta tttaagttga taaaaaaaat actagttgca aatagaggcg
     4021 atatagcagt tagaatcatc agaacatgta aagaactagg tataaaaact gttgctatct
     4081 attctgaaat agataaagat tgttttcata gatatatagc tgatgaatct atttgtatag
     4141 gaccaaataa tataagtaag agttataata atatagaaaa tataatctat cttgctctta
     4201 aattaaagtg tgatgctatt catccaggct ttggattttt atcagagaat cctgaatttg
     4261 caaagcaatg tgaagataat aatattattt ttataggtcc aacaagagaa caaatgattt
     4321 tgatgggaga taaatctaga gcaagagaaa ctatgatgga attgaatatc cctgttgttc
     4381 ctggttcaga atctgttttg aaaactaagg aagaagcttt agaagttgca agagaaattg
     4441 gatatcctgt tatgataaaa gcctcaagtg gaggcggtgg aaaaggtatg agaatcgtta
     4501 gaaaagaaga agaacttttt tcaaattttg atatggcaag ttcagaagct ctagcagcat
     4561 tttctaatag tgatttatat atggaaaagt ttatagaaaa tcctagacat atagaagtac
     4621 aagtatttgg agataaacat tcaaatgcaa tacatttagg tgatagagat tgttctatgc
     4681 aaaggcgaaa tcaaaaagta atagaagaat ctttaagtcc atacctttca gatgaagaaa
     4741 gacaaaaact tcataaaatt gcagtagata tagtaaaagg agtaggttat atcggtgcag
     4801 gtactattga gtttatagtt gacaaagata aaaactttta ttttatagag atgaatacaa
     4861 gaatacaagt tgaacatcca gttacagaga tggtaacaaa tcttgacctt ataaaacttc
     4921 aaatatctat agctaatggg gataaaattc cttttaaaca agaagatatt acttttagag
     4981 gtcatgctat tgaatgtaga ataaatgcag aagattcatc aaagaatttt gctccatcac
     5041 caggtaagat agaatcatta aatttacctg gaggttttgg tgttagattt gatacttttg
     5101 tatatgcagg atatactata ccacctttat atgattctat gataggtaag ttaatatgtt
     5161 gggctgaaac tagagaagaa tgtataagta gaatatatag agctctagac gaaattattg
     5221 tagaaggtat taatacaaat gtagaatttc aaaaggcact tgtaacaagt gaggagttta
     5281 gagaagatac tcatcatacc aaatttatag aagatgtctt tatgaagaaa gaatttgcta
     5341 cattataagg agtaaataga ttatgataaa aaaattttta tcatcaaaag aatctaagta
     5401 tgttacgatt tctttagatg acaactttaa aaagaatagt gtagatgata aattttggac
     5461 ctattgcaaa ggttgtgata gtcatgtatt tagaaaggac atagaagaaa attcatttgt
     5521 atgtccaaaa tgttcaagac attatggctt aagagcaaga aaaagaataa atttattgat
     5581 tgataaagga acttttatgg agtttaattc tgatgtagaa tttcaaaatc cactaaattt
     5641 tcctaaatac aaagaaaaag tagactctta taaggaaaaa acaaaagagt cagaagctgt
     5701 tgtcacaggt tatggaagaa taaatggaat taaaacagtt atttgtgtaa tgaatcctga
     5761 ttttatgatg ggaagtatgg gttctattgt tggggaaaaa ataacttatt caatagagta
     5821 tgctgctgaa aataatttac ctataattat ttgttctgct tcaggaggag ctagaatgca
     5881 agaaggtatg gtatctctta tgcagatggc aaagacttct caagcacttt ctaagcttga
     5941 agaaaaatta ttgccatata tatcagttct aactgaccct actactggag gagtaactgc
     6001 tagttttgct atgcttggtg atattataat ttctgagcca aatacgttga taggttttgc
     6061 aggtccaaga gtaatagagc aaacaataaa tcaaaaactt cctgaaggat ttcaaacttc
     6121 tgagttttta cttgaaaaag gttttattga catgatagta gatagaagaa aaatgaagga
     6181 agtattatat caaattcttg ctatgcacaa gaaatagggc ataaaggagg aactgttttg
     6241 attgaaaata atagtgaaaa aataaaagtc ttagaaaatg atattaaaca attaataact
     6301 atttctaagg caaataatat agatttaagt gataaaataa attcacttaa tgagaaattg
     6361 gaaaagctta aggaagatgc tttttcacat ctatcagcat atgaaaaggt aacattaagt
     6421 agagatataa agagacctac gacattggaa tacatagaac atatttgttc taatttttta
     6481 gaacttcatg gagataggct ttttaaagat gacccttcta tggttggagg aattggtcag
     6541 ataggaaagt ttaatgttac tatagttgga caccaaaaag gaagagatac aaaagaaaat
     6601 ataaaaagaa attttggaat gcctcatcca gaaggataca gaaaagcatt aagacttatg
     6661 aaacaagctg aaaaatttaa tagacctata ataacgttta ttgatacatc aggagctttt
     6721 tgtggtctag aagcagaaga aagaggacaa ggagaagcta ttgcaagaaa cttacttgaa
     6781 atgagtaaac tttctgttcc agtaatcact tttgtaattg gtgaaggtgg tagtggagga
     6841 gctttaggta tcggagtagg aaatgatgta tgtatgttag aacattcagt ttattctgta
     6901 atttctccag aaggactttc tagtattcta tttaaagatt catctaaagc taaagaagct
     6961 tgtgatgtta tgaagcttac aagtaatgat ttatatgatt taaaaataat agataagatt
     7021 ataaaagaac ctcttggagg agcacaaaaa gatgtagaag ctgtttcaaa agaaataaaa
     7081 gcttatattt tagaaagatt aaatcattac aaagatatgg ataaagagga aattatagct
     7141 caaagatata aaaaatttag aaatattgga aaatgcttat agaatataaa gaaatttgta
     7201 gttttttcat aataaaatgg ttaaaaaaaa taaaggtttt cttaaaacga ttatagaagt
     7261 atatttttat aaaagtatac tttaaatgat gattgtaatt agtaaatggc aattatgatt
     7321 tagtacttta ggtaatggat actttaacaa attaacaagg agatgttgaa atggaggtac
     7381 taaaagtttc atcaaaatct aatcctaatt ctgtagctgg agccttggct ggagtactaa
     7441 gagaacgagg cgttgcagaa atacaagcta taggagctgg agctctaaat caagcgataa
     7501 aatcaattgc aatagcaaga ggttttgttg cacctagtgg aatggattta gtttgcattc
     7561 ccgcatttac tgatatagaa atcgaaggtg ataagaaaac agccattaaa cttataattg
     7621 aacctagata atatatctct ttattagaat ttaattaagt tttaaaggtt ggctatagaa
     7681 ataggacact atagtgaacc ttttttactt tatatgtttt attttatgta acttttaatg
     7741 tataatctta agtaaacaaa taagattgga ggaaatatac attatgaaat taaaattatt
     7801 ggtagaagag tatgcagtat gtagattaaa taatgacagt aaaataccaa catggataga
     7861 tacagagaag ttttactcta taactagaac tgatgatgaa ttaagtgttg tatgtttaaa
     7921 taataatatt ccttctgatg tcaagtcaga aaaggagtgg agaatactta agatactagg
     7981 tcctttagac ttttcacttg taggaatttt atctaagtta agtagtttgt tagcagacaa
     8041 caaaataagc atatttgcaa tttctactta tgatacagac tatatcttag taaaagaaaa
     8101 agatatagaa aatgcttgta aaattttaag ctgtaatgga tatgaagtag agtagttaaa
     8161 tacttttaca tgaggactta acttatgatt tatataatcg aaattttaaa aataagtgta
     8221 ataaatattt tttgtttcat aggaatattt attgtatttg gattgatttt tagcattaca
     8281 gaaaatctaa ataataaatt tatattttca tcttttggaa aaactggaat tattgtaact
     8341 ggagctatag gaacgtttgt tcatgaattg agtcatttga taatgtgttt aattttcatg
     8401 cataaaataa attcagttaa gtttttcaga ccaatagaaa gtaaaaatga tggtatacta
     8461 ggatatgtaa gtcatagcta taaaaaagat aatctttatc aaagtatagg aaatttcttt
     8521 ataggaattg cacctattat aggtggaaca ataatgatta ttttaatttt taagatatta
     8581 ctgccagata gttatatgaa agtgactcaa aatatagatt taaagttata tgtaagtatg
     8641 attaataatt taaatataaa tgggtttata cacattattt taggtgatat ttccaatttt
     8701 ataagaataa tgttgttaac acctagtgta tattcattga agtattttat atttatgttt
     8761 ttaatgtatt ctatatctac acatatgtca cttagtagtg ctgatttaaa aaatagctta
     8821 aatggtctga gctttatagt tatagttatt tttgtaataa gttcaattac atacttattt
     8881 ggatttgata acttgaatat atccagttta atagttaagt ataacatatt tataagtttc
     8941 tttatggcaa tagggtttat tttttcaata gtaactctca taatatccta tattttaagc
     9001 ttagtcagcc catttaaaaa ctttagttaa ataaatacat attttactaa tatttggttt
     9061 cgttagaaac atataaaaat attatataaa aaagatttta gtagagcata aaaaatttct
     9121 caatatattt taaaatattt ctataaatat attgagaaat aaaaaagagt acttaaattc
     9181 aactagtctt taaatgtgga tttagggaat agttagtgat aatttcatct aaagcactgc
     9241 taatctctta aaaatgtaaa aaatgattaa attaaatatt tatactcact tggtataaat
     9301 atcaaaaaat aatataaaag atttatattt tagttttagg aggtttaatt ttgaataatg
     9361 ataagaaaat tataaaagta cataataaat cagataaacc tgataatatt gttatcaaag
     9421 aaaatgaact catagaaaaa tgtctacttt tggaaaataa acttcctgta tttatgaagg
     9481 attattttat atatttaaaa ggttctgttg cagtttctac acgacttgct tatttagaag
     9541 atattaattt tttttgttca tatttgattg aaaccaaaga attaacaaat gcagattgtg
     9601 taaaagatat tacagaagat gattttaata caataaaatc aagagatata aatttattct
     9661 taggtgatta ttgcagtaga tattacaaaa atacagaaaa aaacacattg atttttgaaa
     9721 ataacaatag agctttagca agaaaaaaat catccatctc tactctattt aaattcttat
     9781 acagaaactc tcagatagat aataatataa ctgatggatt caatccaata aaacttccta
     9841 aacctcagcc agatgctatt aagcgccttg agatagatga agttgctaag atgcttgaat
     9901 cagtagaaac aggtgaaggg cttacagaga aagaaaaggt atattggaga aaaactaaat
     9961 tacgtgacaa agctatacta gcattatttg ttacatacgg gcttagatta aatgagctta
    10021 gagaactcaa tatctcctct tttaattttt caagaggtga atttaaaata tatagaaaac
    10081 gtgggaaaga agttttaatg cctataaatc atacttgtga acatgtaata aaagattatt
    10141 tgcaaaacga aagaacaaga gatgaactat taaatgatga agaaaaagat gctttatttt
    10201 tgtctcttca aaataagaga attacagcaa aggctataag aacactggta aaaaaatata
    10261 catctattcc acttgatact actagagaca atggatatag tccccataaa ttaagagcaa
    10321 cggctgcaac ttctctaatc caaacaggat tttctatata tgatgttcaa aacttacttg
    10381 accatgataa tgtcacaact actcagcttt atgcagctca taagaaaaat gtaaaacgtg
    10441 atatagtcaa gaattttgaa tggattgagg acgattaaaa atcaggaaca aatgtttgca
    10501 gatataaatg gattatgtta taattaaaaa tataaagtgt atggaaataa aaacaaaata
    10561 tgtttggaaa tgagtgatgt tatgtattta gatctaactg aaaagcaagt cttgatattg
    10621 gaatttataa agtctcaaat catattaaaa ggttatccac ctgctgtaag agaaatatgt
    10681 actgctgtag ggttaagatc tacttcaact gtgcactctc atctaaataa acttgaaaaa
    10741 ctgggataca taagaaagga ccctactaaa ccaagagcta ttgaagtttt agaacgtagt
    10801 aaagtaaatg atgtttctgg agctaatcaa gaaataatag agcttccttt agtaggtcaa
    10861 ataacagctg gagaacccat tttagctcaa caaaacatag aggaatacat tccattccct
    10921 gccagtttag taaagggcag taacaatttt gtattaagag taaaaggcga aagtatgatt
    10981 aatgcaggaa ttttagatga agattacgtt gtagtagaca aaaaaaatac agccttaaat
    11041 tctcagatag tagttgcact tataaatggt gaatctgcta cagttaagag gttttttaaa
    11101 gaaggaaatt taataagact tcagccagaa aatgatttta tggaaccaat tatgctcaat
    11161 gactcagaag ttgaaattgt aggtattgta actggtgtat ttagagttat taaatagcta
    11221 ttaaaaaata cttaaaaaaa taagctattc gtatagataa aatgtgttat ctattgatta
    11281 tagcttattt ttttatgcat ttattttatt ttttaaatga tttaaagttc agaagcttta
    11341 tagataaatt ggttgttttt ttgttatttt ccataacata tatcacatgg gtcatttttc
    11401 ttactttctg caattgtgcc tgataatatt tttttacttc ttgaaagccc actacaattt
    11461 tttgtagagt gataactctt accttttgga gtaaaataca ctatttcttc tgaattggat
    11521 gtattttgtt tatttttaaa agaatcattt ttacttgtat tactgtttga ttttccagag
    11581 tttccatctt ctctaacgcc tttattataa cttccatctt tacaacttgt aaatactcca
    11641 tcacctgttg attcaaatat tatatctgaa cactcatcac ttctatgtac ttttactccc
    11701 atttttttta atttattcat tgtctcctga tgtggatgac catatgaatt acccttgcct
    11761 actaaaagaa ccgcatattc aggatttact ttatctaaaa actcttgaga tgaactacta
    11821 cgagaaccat gatgagctac ttttaaaagg tctactttct caagcttagg gagtatttct
    11881 ttttcggtac cttcttcagc atcacctgta aataaaactt tatcattccc attagtatat
    11941 acaaggacta gagattgttc attagtagta tctcccccat ttgtatttag aacttcaaaa
    12001 taagaatctt ctaaatagaa cttattattt tcaagcggta cacttgggga taaaccctta
    12061 tttgctaaag catttataaa gtccctataa ctctttgttt ctgctgaacc attcgataca
    12121 aaaacatttt ctatattaaa gtttttgaca acagaatcaa gtccacctaa atggtctgca
    12181 tgagaatggg tagctataag atatttaata tcttttacac cttgattgtt taggtaatct
    12241 accattaatc cctcatcatc attatctccc ccatctatca atataaaagt tttaccatct
    12301 tgaatcaata ttgcatcact atttccagtg tcaataaaat gaatttttac attcttatca
    12361 cttgatatac tactagacaa agaatcatta ttatttgatt gattatttcc attatctaat
    12421 gtagtacaac cagttaaata aattgatata gatactatca ataaaagtat caaagtttta
    12481 attttagaat tttttttatt ttttatactt atttctatca caaactacgc ctccatattc
    12541 ataatactct agtaattata acatgatatg cgtttttatg atattttttt
//
LOCUS       DAMKMI010000116        12418 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMKMI010000116 DAMKMI010000000
VERSION     DAMKMI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12418
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.120"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            134..322
                     /locus_tag="PC429_002879"
     CDS             134..322
                     /locus_tag="PC429_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848368.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            582..1781
                     /locus_tag="PC429_002880"
     CDS             582..1781
                     /locus_tag="PC429_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF3848369.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIESDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            1833..2336
                     /locus_tag="PC429_002881"
     CDS             1833..2336
                     /locus_tag="PC429_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848370.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            2443..2760
                     /locus_tag="PC429_002882"
     CDS             2443..2760
                     /locus_tag="PC429_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848371.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            2918..3877
                     /locus_tag="PC429_002883"
     CDS             2918..3877
                     /locus_tag="PC429_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF3848372.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            3992..4777
                     /locus_tag="PC429_002884"
     CDS             3992..4777
                     /locus_tag="PC429_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848373.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDKKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            5069..6388
                     /locus_tag="PC429_002885"
     CDS             5069..6388
                     /locus_tag="PC429_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF3848374.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(6463..6891)
                     /locus_tag="PC429_002886"
     CDS             complement(6463..6891)
                     /locus_tag="PC429_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848375.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(6982..8358)
                     /gene="hydG"
                     /locus_tag="PC429_002887"
     CDS             complement(6982..8358)
                     /gene="hydG"
                     /locus_tag="PC429_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF3848376.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(8359..9444)
                     /gene="hydE"
                     /locus_tag="PC429_002888"
     CDS             complement(8359..9444)
                     /gene="hydE"
                     /locus_tag="PC429_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF3848377.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(9480..10688)
                     /gene="hydF"
                     /locus_tag="PC429_002889"
     CDS             complement(9480..10688)
                     /gene="hydF"
                     /locus_tag="PC429_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF3848378.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLIVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(10703..10954)
                     /locus_tag="PC429_002890"
     CDS             complement(10703..10954)
                     /locus_tag="PC429_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF3848379.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(11335..12375)
                     /locus_tag="PC429_002891"
     CDS             complement(11335..12375)
                     /locus_tag="PC429_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF3848380.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGTLIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         4567 a         1525 c         1890 g         4436 t
ORIGIN      
        1 atgtaatgtc actgatagtt agaatattta agtgctaaaa gatgtgtata aataataatg
       61 atgtcagttt ataatgatat taaattgaga tatactgttt ctctaaatta aaaaataaag
      121 gagtaaatgt gttatgaatt atcatacaaa agaagaattg gtggaagcac tgagagtagt
      181 atcttcaagt attattaatt gtggaaaagg acagaagaaa ttttctgagg agacatctca
      241 tcatacacgt tttaagaata ttatagaagt aatgtatatt tcaaaatcat taataatgga
      301 tgaaattagt aaaagagatt gaagcttatt ttgcaattta acatgtttta atatacattt
      361 tattatataa gaaaataagt aaacatacat gttataaaat attggaataa tatacaatta
      421 taaatttgtt aacactaata tctatatgtt aaaatatagt agaagatgtt agtaaataat
      481 aggatgagtt ctattttaat acaattaaat taagcttagt ttgaaatttg tatctaaaaa
      541 gtaaagtaat ctagaattaa gaaaatggag atagaattat aatggaaaag ttatattata
      601 caaatcagta tataagagat tttacagcag aaataattga aataaaagaa gtagaaaata
      661 agtatcatgt tttattagat aagacagctt tttttcctgg tggaggaggt cagtttggag
      721 acctgggaaa aatagagagt gatgatgtaa taaatgtgta tgaagatgga aaaaaaatat
      781 accatgtatt agataaaaaa cctataaaga tacataaggt aaagtgtagt attgattggg
      841 atagaagaga agatggaatg catcaacatt ttggacaaca tgtattgtct ggatgttttt
      901 ttaaaacttt taatgcaaat acgacaggtt ttcatttggg aaatgagtat agtacagtag
      961 atatagaagg taatttagaa gaggataaaa ttagagagat agagttgttt gccaatgaaa
     1021 ttattcgtca aaatatacaa gtagagacat tacttccaac gaaaaaagaa ctaaaaaaga
     1081 tttggttaag acgtgatttg ccaaatactg atgaagagat aagagtaata aaaataggtg
     1141 atttagatac gaatgcatgt tgtggagtgc atccaaaatc aactggtgaa ttgagaatga
     1201 taaaaataaa gaaatgggaa aaacataagt caagtacaag aatagagttt ctagcaggta
     1261 aaagggcagt ggatgaagta ttgaagaggg atatatattt gactaaaata tgtagatatt
     1321 taagctgtag tgaggaagat gctataaatg gtattgtaaa tctaaatagt aaagtggaag
     1381 aggctttaat taaaaaaaga aggcttgaag aagtagtatc tacatatcag gtcaaagaaa
     1441 tgattgaaaa agcaaataag gttggcaata tttcggtcat taaaaaaatt tatgatgatg
     1501 aagatttgaa atatgtaaat aagatagcaa ataaaattgc agaatatgaa aatagtattg
     1561 cattattagc tgttaaattt aatgataaaa taaatttagt ttttgcatgt tctaaaaatt
     1621 taaaagatat taatatggga gttttattaa aggatgcaat aagtcttata gatggcaagg
     1681 gtggtggaag taaagtatta gctcaaggag gaggtaagaa taatggaaat ttagaggcta
     1741 cacttgatta tgcatttatg aagatagaaa aaactctata aatatattat ataaaaaacc
     1801 ttagtagata aaatagatat ggaggaataa gtatggagca acaaaaggac tacatagagt
     1861 tatatgaaaa tgaagaacta atagagataa gagaagcacg tatggatgat ttggatacaa
     1921 tagcaaaatt taattataat ttggctaaag aaactgaagg aaaagaactt gatatggatg
     1981 ttttaactaa aggtgtaaaa gcattgttat tagatgagag aaaaggaaaa tatcatgtat
     2041 atacagtatt tgataaagtt gttgcacaga ttatgtatac atatgagtgg agtgattgga
     2101 gaaatggaaa tttcctttgg attcagagtg tatatgtaga taaggaatat agaagaaaag
     2161 gtatctttaa ttatttattt aattatataa aaaatatatg tgataaagat gaaaatatag
     2221 ttggaatgag attatatgta gaaaaagaaa acataaatgc aaaagcaaca tatgagtctt
     2281 taaatatgta tgagtgtgat tataatatgt atgaatacga agtaatacat tcgtaatata
     2341 aatttgtaat actgttatac tgatgttata aagtattgat tatattgacg attatatgaa
     2401 ataaactgga tattataatt agaattttta gggggtataa aaatgggcgt tttaaaagga
     2461 atgttttatg tatttttatt tttagactta gtgtcaatat tctgtttctt tttcaataaa
     2521 ggaaaaatag catctaataa aattgtgttt aatgcaatag gagtacttac atttgtactt
     2581 tgttttatgc tttttagtta ttatcctaat aataatctta ttggaaaatt tattgcttct
     2641 ctatttttta tatttggagt tgcaggagtt atgttgaaag aaaaaaattt tttatatgct
     2701 agacttcttt taactgtagt tatagttttt tctacattaa gattgttcgt tattcaataa
     2761 aatgtatttg tatgaaaatt tattaaagtc tacacattta agtgtaggct tttttgattt
     2821 tttgtttgaa aattaaggaa attttaagga aattttaaaa tcgtcttaaa tatttattga
     2881 tatacttaaa atattaatag cgaaaggaga cttatcagtg atagaaagta aaaagtcaga
     2941 aaaattgtta ctgtcaatta tatacataat atttttggta tatattttaa tgattataag
     3001 aataatttta tttagagatg taccaattta cgctattttc aagggaacgt ttaggaatgt
     3061 aaatttaatt ccattttata caatatatca atttatagta gatagtaatc ttgactttat
     3121 gaaagctact gtaaatatta tagaaaatat aggtatattt attcctatgg gaattttttt
     3181 acctatagta tgtaaaaatc taaataaaaa aactataatt atagtaatta tacttgttag
     3241 cctagcattt gaattaacac aatatatttt tgctcttgga agtagtgata tagatgatgt
     3301 tattttaaat tcattaggtg gcataattgg aattaatatt tacataaaca tgaataaatt
     3361 atttcccaat gatattaaca gatttaaggc tataataggt actagtttag ttttaggagt
     3421 tataggatta ggagttattt ctaaaaatta tcataactta ctaactttta aatttagacc
     3481 tgatagaaaa atatcaaaaa tattaattga agaaaatagg gaaataatta aagatataaa
     3541 taaagataca gtagatatag ttgggacatt tgagagcttt aaaaaaggaa taataactat
     3601 taaagcaggt tctaataaca aagtaaagag accagagaat ttaattgatt ctgatggaaa
     3661 tataagaatt tatttaaatg aaaatacaaa attagtatct tatataatta ccaaagaatc
     3721 aaaaatggat atagttaaat atgaggaatt tgatgtgaag aatttagatt tattaaggag
     3781 atatgatact attaatgttt ggattgataa agaaaatcag ccaaaggata aacatggtat
     3841 tatggctagt aaattattaa ttggattgta tgaataaaat attaaacaag aataaaagaa
     3901 taacaaatat aaaaaacgaa aaaactattt ctataattat ttttacgttg tgcataaaat
     3961 aagaacatta gtgaaggagt agagataaat tatggagata ataaaatata tcttgtactt
     4021 ttttatatat tcatttttag gatggacagt agaaagtata ggatgttcta tagcaagcaa
     4081 aagaataatc aacagaggtt ttttaaatgg tccaatatgc cctgtttatg gttttggagc
     4141 ggtaattgtg atatcactat tgggaagatt taataatgta gtaatagtat ttctattagg
     4201 aatgatttta actacgatac tagaatattt tacaggattt attttagaaa ccttatttca
     4261 tgctaaatgg tgggattatt ctgacaaaaa attcaatata aagggtagag tatgtttaaa
     4321 aaatgcaata tactttggtg taatgtctgt tttaataata agatttatac atccatttat
     4381 aaaatatttt gtaagtataa ttccatatag aatattaatt tcaatggcta taattacaac
     4441 actttggact atacttgatt tgattgttac tattataaca cttaaaaaat tagatataaa
     4501 gttaaactta ttagacgata taattacaga ccttaatgat ataaatgtga aattggataa
     4561 atttgataga ggagaaatac aagctttatt taaaacaata aataacgacg aattagaagt
     4621 tagagaaaaa ataaacaaaa taaatagtaa gttggaccgt atagagtcta atattatttt
     4681 acaaaaaaga gttataaaag cttttccaca tataaaacat aaaaaacaac aagagcaact
     4741 agaacatttt aaaaaaatta ttaatgagaa aaggtaatta tacaatttaa tgaagacgaa
     4801 tagaataatg gtatttataa gatgattttc agaggactct ataaaaatag aatagagtcc
     4861 tttatttttt gaaaaataat gtttatttga taaaaataaa atatattgtt tctgaataac
     4921 aaatattttg aaaattataa aaacatatat tgacaatttg tgtcaaaaat actaacatta
     4981 agtatataaa gaattttata tgagaatatc tttataaatc gcaataattt taaaacataa
     5041 atttatttaa agaggggatg atttgccaat gagcgagata actagtcaaa tgataagtac
     5101 agaagaaaaa aaatatgttg ctaaaactca aaagatacct tattatccag tagcatttaa
     5161 atcaggtgat ggtgcaatgc tatatgatta tgaaggtaat gaatatgtag attttttagc
     5221 aagtgcaggc tctgcaaatg taggtcatgg taataaggaa atctcacaag cagttaaaga
     5281 acaaatggat gatataacac aatatacact tgcatatttc catagtgatc ctcctgtaaa
     5341 attagctgag aagcttgtgg aaatagcccc tggagataat gataaaaaag tactatacag
     5401 tgcaactggt tctgcttgta tagatgctgc tataaaatta gcaagagggt atactggaag
     5461 aacaaagata atatctatgt gtgaatctta tcatggaagt acttatggag ctatatctat
     5521 atctgcttta agtacaaata tgagaagaaa aatgggtcca ttgttaccag aagtttatca
     5581 cttccattat cctgataaaa atagaacagc taaagaatgt ttagatgaga tagaatatgc
     5641 ttttgcacac tatctaccag cagaagaggt agctgcaata tttatagagc caattgcagg
     5701 agatgcagga ataatagtgc ctccagtaga gtgggtacaa ggtttaagta aaatatgtaa
     5761 agaaaatgga atattacttg taagtgatga aatacaacaa ggtatgggaa gaacaggtaa
     5821 gtggtttggt atagaaaatt ttggtgttga agcagacctt atagttcttg gaaaatctgt
     5881 tggaggtgga ttacctctag gagcagttgt tggaagaaca gaaataatgc aaagtcttga
     5941 tgcaccagca catttattta ctttagctgg aaacacaaca gtatgtgtgg ctgctttaaa
     6001 atctatagag ataattgaaa aagaaaactt acttcaaaag agtatagaaa tgggagatta
     6061 tattaaagca ggatttgaaa aactaaaaga aaaatacgat ataattggag aaataagagg
     6121 cataggatta tctataggag ttgatatagt taaaggaaaa ggttctaatg aaaaacatcc
     6181 agatgctaca gctaagatat gttatagatg tatacaaact ggattaataa tgatattctt
     6241 aggacaatct actttaagag tgcaaccacc tctagtaata actaaggagc aagtagataa
     6301 agcaatgaat atcatagatt ctgcaataga tgattattta aatggtagaa ttggtgatga
     6361 agtatatgaa gtaactcaag gttggtaaaa taaatattaa atgaaaatag gagttatctc
     6421 aattttagaa aattatttct gaaaagagat aactctttta agttacccac aaataagaaa
     6481 attgtttgat tcattaaaga ctatatttcg gatgtcgttt ttgtgagtat cagagacagt
     6541 attacgagaa taattacctt tattcgtact atctgttaat ttagttataa ttaagatagc
     6601 taaaagaata agaattactg tgtataaagc aataacatat ggttttcttt tatatttgta
     6661 tttgttattg aaaaaagaat ggccataatt atttttattt actttagatg tatttgtatt
     6721 ctttttcttg ttacctttct ttttatcatc cttttttcta tttttagggt aaggaatgac
     6781 tttgtgtgta ttatcaagtt tgatattttt atttttttga atattgtttt ccaggctagc
     6841 ttttttttct ccaaattgaa tagttttatt ggtattgttg ttatctttca atgttaatct
     6901 ccctccctta aactttccct ttagataata ttactactaa cctatggaat atacaataaa
     6961 taatatataa gtaataaaat tttataggta taaatctctt tctccacgct ctaaggcagt
     7021 catattgtta gatactatgt ttcttatatc ttctctttct atcttaccta tttcttctgc
     7081 gataagtttt ttacccataa ttttaagttc ttcatctcca taatcgatta aaaattcatt
     7141 taatgtagta agagcatttg gtgtacatac attatggatt tgtcgacttt tagctaagct
     7201 catgaatcta tcgccagttc tacctttacg ataacaagct gtacaataac taggtatata
     7261 tccatcagta atcagttctt taagcacctc aacagggctt ctatggtctc caacttcgaa
     7321 ttgttctgta ttgtcaccat cttcatatgc tttatatcct ccaacacctg ttagagaacc
     7381 tgcactaatt tgtgatacac catattttag taactcattt ctcatttcag cagtttctct
     7441 agtggacatt atgattccag taaaaggaac agctaatcta gttatagcta ctattttttt
     7501 gaacatatcg tcagaaacta agtgaggaaa atcttctaag ctcatacctt cagctttttt
     7561 caatctaggg aaagatatag tatggaaacc tacaccaaat ttttcctcta agtgttcgtt
     7621 gtgcatcata agtcctagaa cttcaaattt agggtcagat aaaccaaata aaactcctgc
     7681 accaacatca tcaacaccag cttccatagc tctatcaaaa gctgttaaat ggtaataata
     7741 gtcatttttt atagattgac catgcatttt tataaatgtt ggtttgtgat aagtttcttg
     7801 gaataatata taagttccta ttcctttttc ttttagtaat ttatactcat ctacacttgt
     7861 agctgctatg ttaacgttta cccttcttat atttccgttt tcattatatg ttgaataaat
     7921 tgcatctaag caatctaaga tgtaatttat atcacaattt ttagggtctt caccagcttc
     7981 tagagcaagt cttttatgac ccattttttc taatatttta acctcttccc ttatctcttc
     8041 catggtaagt ttttttcttt taaatttgtt gcatttacta aaaccgcaat aaacacattc
     8101 atttacacag tagtttgata cataaagagg ggcaaataaa accactctat taccatatat
     8161 ttcatttttt atttgcccag ctatactaaa taatctatct agttgttttt tatcttctac
     8221 ttcaagaagt gtagctatat ctttataact aagtttttcc cttctatcag ccttatcaag
     8281 taccttttca atatcatcag aagttgaatt ttttgcatct tctaataaag aattaataag
     8341 ttcatggttt atgaacattt acaaacgcct ccattcaata ttatcaccgc gagaatagtc
     8401 aacttcaagt cctaatgact tgatgttttc ttctaaactt tggtgatatt cacaagcttc
     8461 ttgaccagta aaggctttgt tatcgtaaag agaatattgt tttctaaatt cttgaggaga
     8521 aaggtttggc ataattacat tgcaaccagc taaaagacct gcatttctac cactagggtt
     8581 tatagatgcc aaggctgttg tagcgggtaa taaaactttt ggaagtagaa gtcttgtaat
     8641 tgaaagcata agtaatgtct tctctaaatc tccatgttta taatccttaa aaattgtatc
     8701 atggtgtgga atgaaaggtc ctattccaac catatgagga tttaactctt ttaggtataa
     8761 taaatcacga acaaggtctt cgttgttctg aaatggtgat tcaaccataa atccagcacc
     8821 tacctgataa ccaatttctt taaggttttt taggcattct tttctagttc taagctcaat
     8881 gtttggcgga tgtaattttt tataatgact gtctgtagca gtttcatgtc ttaaaagata
     8941 tctatctgca ccacataaaa agtatttttt atacgaatca taactctttt ctcctagtga
     9001 tagagttatg gcacaatcct catatttatt ttttatactt gaaacaattt cacatatttt
     9061 atcatcagta aaatatgcat cttcaccacc ttgcaaaacg aatgttctgt atcctatttt
     9121 ataacctata tcacaacatt caagtatttc atcaagtgaa agcctgtatc ttatgatatt
     9181 tttattagaa gattgtattc cacaatagta gcaattattt ttacaatagt ttgtaagctc
     9241 tataagtcct ctcaagtaga ctctatttcc ataatgttta tttctcatgt cgctagcttg
     9301 tttataaaga tagtttctta aattttgttc gaagttatat tcctcattgt cgatattttc
     9361 tattaaatat agtagttcat caaaacttaa tgaattaatg ctgtaaagtt tatctataaa
     9421 gtatttaatc ttatttttag tcatattcaa atatataatt attaagtata gttaaatttt
     9481 taataattga aaagttcaag tgatctttcc aaaatacctt ttacatatgc tattaagata
     9541 ccatagttta caataggtac attgaaagat tttgcctttt ctattctaga taacattcca
     9601 gctctattca tcatacaagc tccacagtga actacaaggg catatttatt tatatcttca
     9661 gtgaaggata ctccagaact aaattcaaaa gtaatgtttt tgccagtttt ctttctaatc
     9721 atattaggaa tttttactgt tccaatatca tcagtttgtc tatgatgggt acatccttcg
     9781 gccatcaaga ttttatcacc atcttttaaa ttttctaaag cataagcgcc attaatcaat
     9841 tctttaaggt ctcccttttg tctagcaaac aatatagaaa aagatgtaag aggaatatct
     9901 tttggtgtat ctttatctac ttgtggaaat acttgagagt cagtaataac aagttttggt
     9961 tttttgccta aattagatag agtttctttt aaactatctt cttttgtaac tatagaaatt
    10021 gcaccactat ccaaaatatc tcttataact tgctgttgag gaagtataag tctacctttt
    10081 ggagctgctt tatctattgg aacaactaaa acaattaaat catttggttc aattaaatct
    10141 gataccaatt tgaactcagt gctatcctta ggcaaaactt ttattatttc attttttaaa
    10201 ttttcaattc caattttatc tgttgatgag acagaaacaa ctggacattt aactttattt
    10261 ttagttaagt ttaaaatttc tgattgattc ttaatagtat caattttgtt gagaattaga
    10321 atatgaggga tattcttatc attaaacttt tcaattagtg acaaatcttc ttgtgatata
    10381 ccaatctggc aatccactac tagtaaagct atgtctgtct tttctaaaac atctagtgat
    10441 ttacctattc taagttcacc aagttcacca acatcatcaa gaccagcagt atctataaga
    10501 acacaaggtc ctataggaag gatttccatt ggtctaaata ctgggtctgt agtagttcca
    10561 gcaatatctg agactattgc agcactttga tttgttatag catttattat gctagattta
    10621 cctgcatttc ttttaccaaa gagacctata tgtactctta ccgattgtgg agtagaattt
    10681 agactcattt gtactcacca ccttaaactt ctttttttga tatactagtt tttacagata
    10741 catgaggtat gttgcccaat ttaccagtca aactattaat agtatccatt tcgccaacaa
    10801 ctgctataca tacgattgag acaccttctt cttcgcaagg aatgcccatt cttcctttaa
    10861 taataccttt aaaacctgat acaacctcat tgaatttaaa ttgacattct tttggctctt
    10921 ccaaaatagc acttataact gcaacctttt tcataaatat cctcctaact cagaaaatat
    10981 gtcttaaaca aatccaaaat caaaaaactt ttctttgtaa agaaaagttt ctacaaaaga
    11041 aaagttatta attacacaag tataatgtgc tatattaaac tatttcctta cagattagat
    11101 aactcttttc tggtagggtt agcttgtaaa aatttaattt aacaacaagt aactgaagaa
    11161 aatttttata caatttatat aaattataat aaaaattaac taaagaaaat tgaagttact
    11221 tatataatag tagtaaattg aaattaagtc tatagaaaaa ccaatttttg tataaaaata
    11281 aaaattaaag ttaattattt gttaagaatt gactttggta aaatatataa ttaattattc
    11341 tttaagcata taatttataa gtaattttag tgtattttca atagcttcta tatgtgttct
    11401 ttcataatgg tgactattgt ttgttccagg accaacacat gctatttgaa catcttctcc
    11461 ccaacgaata gcttctgaag catctgaacc atagaaagta aatacatcga cagcgtagtc
    11521 taaattattg tcttctgcaa catcaacaag cttgtttctt aaattaaaat catataatga
    11581 taaattatct tttgcaacta tagttacacc atgttcacta gatgtttgac ttggtccagt
    11641 aggtgctata tctattgcta caaattcttt tgttttttca ggtaaacatt tagaaacacc
    11701 atgtccaatt tcttcataat tacttatata aaagttagta gtatgcttag gtgttaaatt
    11761 gttttcttta aaatatctac atatttcaag aaccattgct acagctgatt tattatctat
    11821 atatctagat tttataaaac cagattctgt agcctcaaat ctatggtcta ggcatacaaa
    11881 atccccaaca cttattccta aatctaatac atcttgcttt gtaaatactt tttcatcaag
    11941 tctaataatc attgtttctt catttctttc agtggcagct tttgtttgac catatatatg
    12001 agtagaagca tacttaaata caacacttcc tctgattttt ttaccttttc tagttataac
    12061 agtacagttt tctccctcta tagctgccca acatccacca ccaactttat ggtatttaag
    12121 agtaccatca gatgatattt cttttactat agctccaagt gtatccatat gtgcagatat
    12181 tgttatttga tttgaatcat cttcaccagg aagagtagct ataagtgtac ctttttttgt
    12241 aagaatagtg tcaagaccta aattttcaaa atcttttttg atttctaaaa ctgcattttc
    12301 agtatatcca gctgggcttg gaatgtccaa atattttttc attaaattta tagtattagt
    12361 taaatttaaa ttcatattgt ttcctcctaa ataaatatat ttatacaaat tataacat
//
LOCUS       DAMKMI010000117        12378 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMKMI010000117 DAMKMI010000000
VERSION     DAMKMI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12378
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.121"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            316..1251
                     /locus_tag="PC429_002892"
     CDS             316..1251
                     /locus_tag="PC429_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF3848381.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1343..3214)
                     /locus_tag="PC429_002893"
     CDS             complement(1343..3214)
                     /locus_tag="PC429_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF3848382.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3404..4330
                     /locus_tag="PC429_002894"
     CDS             3404..4330
                     /locus_tag="PC429_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF3848383.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4469..4615
                     /locus_tag="PC429_002895"
     CDS             4469..4615
                     /locus_tag="PC429_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848384.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4720..5244)
                     /locus_tag="PC429_002896"
     CDS             complement(4720..5244)
                     /locus_tag="PC429_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848385.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5297..5521)
                     /locus_tag="PC429_002897"
     CDS             complement(5297..5521)
                     /locus_tag="PC429_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848386.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5718..6842
                     /locus_tag="PC429_002898"
     CDS             5718..6842
                     /locus_tag="PC429_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF3848387.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7316..8140)
                     /locus_tag="PC429_002899"
     CDS             complement(7316..8140)
                     /locus_tag="PC429_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848388.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8488..9543)
                     /gene="ispG"
                     /locus_tag="PC429_002900"
                     /gene_synonym="gcpE"
     CDS             complement(8488..9543)
                     /gene="ispG"
                     /locus_tag="PC429_002900"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF3848389.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9725..10729)
                     /gene="rseP"
                     /locus_tag="PC429_002901"
     CDS             complement(9725..10729)
                     /gene="rseP"
                     /locus_tag="PC429_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF3848390.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10742..11896)
                     /locus_tag="PC429_002902"
     CDS             complement(10742..11896)
                     /locus_tag="PC429_002902"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF3848391.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(12060..12263)
                     /locus_tag="PC429_002903"
     CDS             complement(12060..12263)
                     /locus_tag="PC429_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3848392.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
BASE COUNT         4115 a         2085 c         1292 g         4886 t
ORIGIN      
        1 tatatacttt atgtgtaaat tatggtaaaa atattattta atcaaatcaa aacttgattt
       61 atatacctgg aaaaggtatt atattaaaga gtgcttaaaa tacgcatttc ataaatagct
      121 atatataaaa taataaataa ggagagtgat gggcaatgga tactagtccg ttgcggagag
      181 aattttttac attttcatca cttaataata tctaaaatca aaactaatac atatattcag
      241 ttatataaat tagttaatta atagatacta ttaaatgatg agatttatat aaaactgaat
      301 aaaggatgtg ttgccatgat aagatattac aaaactatag attcaaaatt agagaaactt
      361 agtttttttg aagatggctg ttggataaat cttgttgaac ctaatcatag tgaaatcaac
      421 gaaatttcta atttattaaa tatagatgta gaaagcatag aatctgcact agatgaagaa
      481 gaacgctcaa gaatagatgt tgaagacaat catactctta tacttataga tataccagta
      541 gacgaaagtg actctaattc ttctcattat actacaatac ccttaggtat tatactaact
      601 gaagaagcta ttgtaacagt ttgtgatgca caaactaaga ttttaaatga ctttatagtt
      661 ggtcatatta aagatttttt tacgtttaaa aagactcgct ttttgcttca aattcttcac
      721 aaaaatgcag cttattactt gcattactta agaaaaataa ataaaatgac aatcataata
      781 gagcgagaaa tttacaaatc aatgaaaaac aaagaattgg ttcaattatt ggaattagaa
      841 aaatctttgg tatacttctc tacctcacta aaatctaatg aacttgtttt aaacaaaatg
      901 gttagaactg ctggtataaa gaagtatcca gatgatgaag acctattaga agatgttata
      961 gttgaaaata gacaggctct tgatatggct aaaatatatg gagatatatt gagtagaatt
     1021 atggatgctt tcagtgcaat tataagtaat aatcagaata atgttatgca aattttaaca
     1081 gtagttacac taattttctc aatacctaca ataatatcag gattctttgg tatgaatgtc
     1141 atcaacatgc cattttctaa taacccaaac ggattttgga taatacttct tattacctct
     1201 ataatatgta tagtgattac atttttcatg tcaagaaata agctattata aaatattaat
     1261 aaaaaagcca ttagaattaa ttgatttaca tttatcctaa tggcttttat atatttaaat
     1321 ctatataatc ttataatact ttttaactat catgttcttc atctataatc atttcagttt
     1381 caagcccttc tagcattgaa tctaaacttt cttttatatt tagtatataa attggtacat
     1441 actcttcttc atctattacc tcttcttgca acttaaatgg aggaattaat acttcatcaa
     1501 agtctatatt taaatttgga aagccttcta ctatctcttc acaattatcg tactcttcat
     1561 ttgctaaaga aatgtacata tctaggctct ttttataata aagacctgca agcatatact
     1621 cacacttctt acttccatat ttaatacctt ctttataata tttctcagct tctgcgtcat
     1681 catttaattt taaataaatt tgacctaaat tatatattga ttcaacacaa ccttgtgctc
     1741 tagccttttc ataccaaaat ttagaattta cataatcttt atagaacata tcataaatta
     1801 atcctaccat atgttgagca tacacattat tttcattggc aggtgtatca aacatcattt
     1861 tagactcttc caatttacct tctctaaaat aaattctacc tagatgtata cgagcattca
     1921 tatggttttt gctacttgcc atttcaaaat actttctagc attttttgta tttcctaagc
     1981 tctcatatat acaccccaat ctataactag acctcgtatc tccattttca gatgctttct
     2041 tataccaact tattgcttct tcaatattat ttaaatcttc atagatattt cctaatttga
     2101 tttgacaaga tacattattt ggaatcctcg aataatacga aatcgctttt tctatatctt
     2161 gattctgata atataaatcc cctagatttt caagggaact tttgcatcca acagctatag
     2221 catcctcata atatttaata gcattttcat aatctttttc ttcaaaatat atacctgcca
     2281 gattattttt tgcttctgtt atatcatctt cagcagccat tttataataa gtttttgcct
     2341 tctctatctc attttctcta ttatataaat tagcaagtct atacttagaa agtgtatgat
     2401 ttttttctat tgctttctca taataataaa ttgcatctcc cagctcttct tttctatcat
     2461 atacatatgc aagcctatac tcagacttca catcatctct atcagcactc tttttataat
     2521 acttctctgc atcttctaaa ttatcattta tttccataat catacccaag ttaaagcaac
     2581 ctttcatatg acctgacttg tacaactgtt tatataaact tactgttgat ttattatctt
     2641 tcttttttcc atttaatatt gctaaattat attttgcatc atcatctaaa agcacagaac
     2701 cttttttata gtattcatat gcttcatcat ctttctccaa aaaagtatat aataccccta
     2761 aattaaaaca agctttacct aaattcttgt ctgctgctgt ttttaaccat ttttcacacc
     2821 ttacaaaatc tttcatttta aaatggcata cacctaaatt attttgtgct tgtaaataac
     2881 ctaaatttgc tatcttttca taccaatata tagcatattc atagccatct aacttatagt
     2941 acatattacc taaatcaaaa gcagcatctt cacttccact tttatatgcc attgaatacc
     3001 acttttctgc ttctttaaat tgttttttac tttcatatat atttcctaaa gtatattgag
     3061 attttacgta attatccaat gcagcactct tatacaactc tattgcgctt tccatcttac
     3121 ccatcttttc aaggagggca cccttagtat gtacgacttt cgggtcatta gatatatttt
     3181 ttattttttt tatagaaaat tttgtatttt tcattattcg ctacctaccc attttataaa
     3241 atatattctc tcaaacatta ttatacatta taatctacaa ttaatgctat aatattgaat
     3301 ttttaccttt tgtcactata ttgtaacatt aaattttaaa ttttataatc attagatttt
     3361 attatgtaat tggtatataa tgtatatata ggggggacat tttatgaatt tcagcaatat
     3421 ttttatccaa gttgcagttt tatttataat tattttagtt ggatattttg tcagaaaatt
     3481 caacttatta gatgaccact gcacatctaa gctttcaact ttgacaatga cagttttttt
     3541 accatcaatg ataattagct ccatgcaaat taactttgat agtaaaatga tacaaaaaat
     3601 actattgtta ttatttacat ctttgattat gtatatagtt tcaattataa ttgcattttt
     3661 attgaaatac attttaaaat gtaatgataa aaaagatttg ggaatatacc agtatatagt
     3721 tgttttttca aatgttgctt ttatgggtta tcctgtaatt gaagcagttt taggtcatga
     3781 agcaatattc tatactgcaa tattcaattt gccttttaac ttattttctt tcactttagg
     3841 aatttatctt ttatctaaag gcagtacaag tagaggtttt tcaataaaat cacttataag
     3901 ccctgctaca atagctattg ttattggact ttttttattt gtaacaggac ttaggttacc
     3961 ccaatttata aacgagccac ttgagatgtt gggaagtata actacaccaa tatccatgat
     4021 tatcattgga agcttacttg ctaattcttc agctctcgat tgttttgtca acaaaaaatt
     4081 atatatagtc acttttataa gattattggt acttcctgtg attgtgtatt ttattctaaa
     4141 aggatggata aatgataaga tgattttagc tataccagta gtaatttctt caatgcctgc
     4201 tgctgcaaat actgctataa tggctaatca atatgactcc aatataacac tagcttcaca
     4261 atgtgtgttt tttacaacac tattttcagt tatatctata ccttttataa gtattttact
     4321 cttatcttga tttatttttt tatgtattat ataataacat attcaataaa ttacaaatat
     4381 aaacatttta ttgtagtata ttgatggaat tttttcctag atatttgtca ataatatcta
     4441 tatgtattat agaataggag gcttaaatat ggatatggat ttactcttta aaatagtatt
     4501 tgctatagtt gctataggtg tttctgtaaa aatcattaaa actgttgcta gtttagcttt
     4561 taaaatagcg ttgatattgc taataatttt atttgtatat agaatgtttt tgtaaacatt
     4621 taaaaataag ttttttaaca caatagagaa gtaaaataga ttatctttaa tgtaaatttt
     4681 ttattaaaga cactctattt tacttctcta ttagcttatt taaattaaat ttttattata
     4741 tcttcccata atataaacat cttcaaattt tccatctctg attacagcat attttttagt
     4801 tccctctatt tcaaaaccta gctttttata taaatttaat cccttttcat tatccacaat
     4861 tacttctaat cctactctag ttaacataat ccaattatct gctatatcta aaagttgttc
     4921 cataagcttc ttacctactc ctcttccctg ataattctct gcaacactta ttcctatttc
     4981 tgcgctatgt ctcaatcgtg gagaagaatt tacatgtaac ccagcaaaac ctattacatc
     5041 tttttctcca ttattattag tttcagctac aaacatataa tcattttctc ccaaagaagc
     5101 taacatagct tcagttttat cgactctgtc actcattaat gccaaagtat ttctcataac
     5161 actgtatgtt cttctaattt cattaaaagc ttgtgcatct tctcttctta taggtcttat
     5221 tgttatatct ataggcttac tcatattgga ttccccctca aacataatct ttatttttaa
     5281 ttaaaatttt acatctttat tttatactgt tcaaatctat gtatgcttct acctgaggta
     5341 catatatcat attatctttt atatgtgtac ttccaaattc ttttaattct gtaccttctt
     5401 tatccttagt tattttatat ctaatggatt ccataccttc aactacttca gcatgaaaaa
     5461 acataccttc atattgttta aatctaaaat taaaattgtc tttagtaagt tttattatca
     5521 tatttttatc tactccttat ataattgata tttttcttat tattagttgt atatatactg
     5581 cttattcata ttaatatatt ataacttatc atttatttag tataaagtta taaatagata
     5641 tatttatact tattttacaa tctttcttca attgatatat aatatgttta tataaactaa
     5701 tgtatgtgag gtgaaatatg agcaaaaaaa caaaatattt ttctttaatt ctctttccta
     5761 ttgctttgct tttaaatttt atagccagca aagttccaaa tattgttgaa aaatattact
     5821 cccaattcat agataaaata attgtacaaa tattgagtaa agtttctgga attttcccat
     5881 tttctctcta tgagattaca atgtacataa tagttatatc tatcttctta tttttatgtt
     5941 gtactttatc tacaattttt aataagaaaa aaaaattaaa catatattta aaaaattcca
     6001 ttttaaatat attatccatt gtatcaatag tctactttct ttttgtagtg ctttggggaa
     6061 taaactataa taggattcca ctagaaacca cacttataaa taactataat ttaaaaaaca
     6121 atacctctat ccaatcaaaa caatatagtg ttaaagagct tactaaatta tataaattcc
     6181 ttgtaattaa agcaaatgaa actagaaaac tcacattaca agataaaaat ggtattgtaa
     6241 aatctaatac agattataat ggtgtcataa atagagctca actaggatac gataatatct
     6301 tggatatatt acctggtgtt agtggtagtt attctaagcc taagtatatt atttcatcaa
     6361 atctaatgtg ctatacagga ataactggta tatactttcc ttttacaggt gaggcaaatg
     6421 taaatatagc aatacctgac ttatatattc cttgtacagt tgaacatgaa atggcacatc
     6481 aaaggggatt tgctagtgaa gatgaggcta attttatagc gtatcttact tctataaaac
     6541 accccaatat tgattttaac tattctggat atattttagc tttaaattat actgcttctg
     6601 ctttaagtaa ggttgattat aatgcatatg ttgatatttc tgctggcatt tcagattctg
     6661 tccgtagaga tttaaaaaat gaaagtgaat tttggcaaaa atatgaaggt aaaattaatg
     6721 aaatttctaa tgagtttaac aattcttact taaaagctaa tagcgttact gaaggtactc
     6781 agagttatgg taaaatggtt gatttattgc taacttatta tgaattatat ccatataact
     6841 aaacactaca attcaaaatt ttattcatat acagctgata ttaaatcagc tgttttctat
     6901 ttaaataaat tatttaactt caagcaatct tatataattt gatagtactt atactatctg
     6961 tataacattt caattctaac tattttagtt aaaacatttc tattatcttt tttaataaat
     7021 gttttaacta aaatttttaa aacataaaac ttccacatta tttcactatc tatataaaaa
     7081 tctaaaaatt cacatataaa attacataac taaaaaatca taaatttata gaaaacatat
     7141 attcaaaata aaaaagcatg tatcaaaact tgacacatgc ttataatctt atgtatatat
     7201 atcattttgt cacaataaaa atttacttgt aacactgata tttttaaaca aaatagtcct
     7261 tatttaatta aattaacatt aattaaattc gtactcattt tgctatcatt aaacattact
     7321 tatagtaatt ttctaataca ctatttgaaa cttccttccc ttgaaaactt ttcaaccaat
     7381 tctcatatgc tgagtttttc catacactac catcattaaa tgttacttgt tttacacaag
     7441 atataatgta ctttacacca tgctctccag ttgattgttt ccatttatca aacaatagaa
     7501 aggcatcatt ttgattctta cctggtgcta ttgcattttg ctttgcatat tctttctcca
     7561 tttgtttcca ttctttcaac caattttcta cccatgcctt gccttgttgt ttttcaaaca
     7621 tacttattat atcctgtggt ggtgattgtt gcatttttct ataagtatgt gaatatgatt
     7681 ttggagacac aggagatatg cctgtaacaa taccatagtc tacaccagct gggctaaatc
     7741 caacacttcc tacttcgcca tctgctgcca cattttgagc atcccaatat agctcaagtg
     7801 gttttccatt tttatcatat gctaagcatg taatttcata atccataatt gccttttcac
     7861 tgctatttgt taagcttata acattgctag gccaatgata tggagtatca tcatactgta
     7921 ttttgctatt atttataaat aatggaatat tctcattata aacaagacct ttactacttt
     7981 gaatatctga taccttcttt gtgacagtac ttaccgtatt tgctaagtat acatttgttt
     8041 tttctgaatt ggtcgtacta agagaaaatg ttgttgtcgt tccagtaatc agcaaagcaa
     8101 caactaaaat aggtaagcta gatatttttt tatatctcac aattcttcac ctctttttct
     8161 attaacttat aataaaacaa tttaccaaaa cattaaaatc tatgttaaca taatttacca
     8221 actgttctag taactatatc ctatcatagt tactatcaag tttcaatact attgatggaa
     8281 attttcacta tattaaatct ttttttaatt ttataaattt acaatatgat ttaactcata
     8341 catatatagt caaattagaa tataagtttc ttacatttaa tgaagtttat aaaataaaaa
     8401 atgactagat aattaaatac tgtaaataaa aacttattac aaaagtattt aaaaatctag
     8461 tcaattaata tatatatgaa ttgtatacta taatttttca atctcttcta gcaactcatc
     8521 aactaatttg ttaccatcaa tcattctaac tatttcaccc tttttaaata aaagaccttc
     8581 acctttaccc ccagcaatac ctatatctgc ctctctggct tcaccaggtc catttacagc
     8641 acagcccata attgcaacag tgatattttt ttccatacta ccaatctttt cttcaacctc
     8701 atttacaaca ctaattaagt ctatattaca tctaccacat gtagggcatg atataacttt
     8761 aatcttatca tttaaaagtc ccaaacttct taaaatctgt ttaccaacaa taacctcttc
     8821 aacagggtcg cctgttaaag atattctaac agtatctcca attcctttaa gaagaagtgc
     8881 tcctacccct atagaagact ttattgttcc tttgtgaaca cttccagatt ctgtaattcc
     8941 aatatgaaga ggataatcaa ctttttttga tattaattca tatgcatcaa cagttttata
     9001 aatatcggat gattttagag atataactat attatgaaaa tctaagtctt caagaatctt
     9061 aatatgtccc attgcacttt caactaatgc ttcagcagtt gcagagccat acttttttaa
     9121 aagttctttc tctaaagaac ctccattaac tccaattcta atttttaaat ttctttcctt
     9181 gcatttctca actaccatct taactctctc tatacttcca atattaccag gatttattct
     9241 tattccatcc acaccttgtt ctatagcttc aagggctagc ttataatcga aatgtatatc
     9301 tgcaataatt ggtatattta cactagactt tatctttgct atatttttag cagctttaat
     9361 atcaggaaca gcaactctaa ctatatcaca accagcttcc tctaatctct ttatttgagc
     9421 tattgtagca tctgcatctc ttgtatcagt atttgtcata gattgtatac ttataggatt
     9481 ttctcctcct atttttacag ttcctacact tacttctctc gtctttcttc tctcgtaact
     9541 catattccac ctctatatat tattttatat tgaatctctt taaatattaa aatattataa
     9601 cctacatgta tattataaat taaaaagcta tctagttaaa taagaataaa tttcatatat
     9661 aaaaattaat ttattcttat ttaaaactac atagcctatt ttcttaaaat ctctaaaaac
     9721 taaatcattg aaataatctt agtatatctt tatatgtgat aaaaagcata aataacatta
     9781 aagctccaaa accaacaaca tttatcatgc cttctttatt tgggtcgagt tttttaccac
     9841 ctctaacagc ttctagtaac agcataagta ttctccatcc atcaagtgct ggtattggta
     9901 atagatttac tatacctaaa tttaagctta tgacagctgc taaatacact acatttatga
     9961 tgcctgtacg agcagcatct gataccatac caataacacc tactggtcct gctacagcat
    10021 tgccaatccc tcctggtaca cgtcctgtaa tcatttgacc tagaaatgtt agcatctgtt
    10081 ttgtcatatc tacagtagtt ttacatgcat ttactattga ccccaaaaag tctttttctc
    10141 tttgtggttg aatccctatt tcatactttc cattgttatt tttaggtgtt acatttacaa
    10201 ctttatcact tccatctcta gttatactaa gttttaattc tccaccagaa gaagtattta
    10261 ttatatttgc aacttcatcc caagtcttaa cagaattacc attaatttta tttatcttat
    10321 ctcctacttg aagccctaca gcctgtgcag gagtatctgg cattatcttt cctaatttag
    10381 tcgttggaga acctatatac aaaaatactg gaactaaaag tattgctgct aatattatat
    10441 tgaatattgg tcctgctaca atagtactaa atctttgtaa tatagacttg tttccaaaag
    10501 aatttggact tatttgttct tcatcttcac cttccatgct gacatatcca ccaattggaa
    10561 gtgctcttat agaatactct gtatcttttt ttacactata tatcttaggc cccataccta
    10621 tagaaaactc atttactttg ataccacttc tttttgcaaa tataaaatgt cctaactcat
    10681 gtattaatac aattatacta aatagtatta atgcggctat tatagtcata aattcacctt
    10741 cctatttttt tatcagattt gcaacataag ctctactcca tctatctgtt tctaagattt
    10801 cttctaatgt tggttcactt atgctactat gaacttctaa agtcttctct atgtaatatg
    10861 gtatgtcata gaaaccaatt ttatcctcta aaaattcact tactaaaact tcatttgctg
    10921 aatttaaaac agaggaataa gttcctccca tttttaaaca ctcatatgcc aattttaaac
    10981 atggaaaagt ttctaaatct ggttcttcaa aagttaaagt actaaatttt gaaaaattca
    11041 ttctttcaaa gctactttcc attctatctg gatatgtaag agcatactgt attggtagtc
    11101 tcatatctgg acatcctaat tgagcaatta ttgagctatc tgtatactgc accattgaat
    11161 gaattatgct ttgtgggtga actactacat ctatattttc ttgttctact ccaaataacc
    11221 atctagcttc tatcacttca agtcctttat tcataagcgt tgaagaatca atacttattt
    11281 ttcttcccat actccaattt ggatgcttca aagcttcatt tttagttata tttgcaagtt
    11341 ctcccttttt ctttcctcta aatggaccac cagatgctgt aagtattatc ttttctatat
    11401 tttttttatt ttctccattt agacattgaa atatagcact atgttcacta tcaacaggaa
    11461 gaatatttac attgtatttt tctgcttctt tcattacaag ttcaccagca gttaccaacg
    11521 tttctttatt cgctaatgct atgtctattc ccttctttat agcacaaaga gttgggacta
    11581 atcctatcat acctactaca gcagttaaaa ggacatccac ttcatcaagt gatgatatta
    11641 tttttaatcc ttccattcct gctaaaactt ctatttctat atttttaggc aacatttctt
    11701 ttaatttcaa tgctttattt tcctcaaaaa ctgttacata tttaggttta aactctacta
    11761 tctgttctag caataattca atattactgt tagctgatat tgcaactatc tcaaacttat
    11821 ctctattatc tctaactaca tccaaagttt gtttacctat agagcctgta gaacctaaaa
    11881 ttgatatctt tttcatataa taatcctttc taataataaa tataccctgt caaaacatta
    11941 taacactact aaaacaatta tttttactat tcatagaaat ttcatatatt attccacaga
    12001 ttaaaattaa tctattattt tttgtatttt gtggaatatt ataaaatatt ctagtattat
    12061 taaatgctct tttttatttc cacaacatct tctattttac agtctagtgc taaacatatc
    12121 ttctccattg tatccataga aacagattta tcatttccta gtctgcctaa aacagaaata
    12181 cttatacctg atatatttac aaattccatt ttctttattt ttctgtcaat taacaatttc
    12241 cataatggag cgtatgaaat catgttatca cctcgcaaaa tatattctat cacattttat
    12301 ctattttgga aaattttcat ctatttagat aaataattat ttatctaaat agatttcaat
    12361 tatagtttat taaatata
//
LOCUS       DAMKMI010000118        11822 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMKMI010000118 DAMKMI010000000
VERSION     DAMKMI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.123"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            180..1220
                     /locus_tag="PC429_002904"
     CDS             180..1220
                     /locus_tag="PC429_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3848393.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1576..2898
                     /locus_tag="PC429_002905"
     CDS             1576..2898
                     /locus_tag="PC429_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF3848394.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSIITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3195..4556
                     /locus_tag="PC429_002906"
     CDS             3195..4556
                     /locus_tag="PC429_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF3848395.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTAIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4629..6023
                     /locus_tag="PC429_002907"
     CDS             4629..6023
                     /locus_tag="PC429_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3848396.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6085..6555
                     /locus_tag="PC429_002908"
     CDS             6085..6555
                     /locus_tag="PC429_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF3848397.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6558..8774
                     /locus_tag="PC429_002909"
     CDS             6558..8774
                     /locus_tag="PC429_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3848398.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8857..9528
                     /locus_tag="PC429_002910"
     CDS             8857..9528
                     /locus_tag="PC429_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3848399.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9891..11105
                     /gene="dpaL"
                     /locus_tag="PC429_002911"
     CDS             9891..11105
                     /gene="dpaL"
                     /locus_tag="PC429_002911"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF3848400.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11232..>11822
                     /locus_tag="PC429_002912"
     CDS             11232..>11822
                     /locus_tag="PC429_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF3848401.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4469 a         1363 c         2397 g         3593 t
ORIGIN      
        1 catttttgta aaatgaagaa agtatatcta tcaaattaaa actaagtaga tatacttttt
       61 attaagtttt gttttaaaat aatattatta aattataact aagcttttct gttatgatat
      121 aattaaatag ataatgtgaa tgattattat atagaatact tatttggagt tgatatttta
      181 tggacaaaca cagtgattca gtaatgtatg gtggaatgga agtatataat atttggggaa
      241 caacaacagt agactccata attggagagt ctgatgtaat gcgtgctctt aagaatagaa
      301 tttgtaaaat tggaaatagt gattctactg tagctataac tggagaaagt ggcactggaa
      361 aagaattaat agcaagagcc attcatctcg caggaaacag agggaataaa acattcgttg
      421 ctataaactg tgctgctata ccagaacctt tactagaaag tgaacttttt ggatatacaa
      481 aaggagcttt tagtggagca gattcaaaag gtaaaattgg taagtttgaa ttggccaatg
      541 gaggggttgt atttttagat gagataggag atatgccaat tcaactacaa tcaaagttat
      601 tgagggtatt acaggaaaag aagtttacga gaataggttc aaatgaacta attgatatag
      661 atgttagaat catatcagct actaatagag atttatttga gttggttaag gaaaataaat
      721 ttagagaaga cttatactat agattgaatg taataccttt agaagtgcct ccacttagag
      781 aaagaggaga cgatatacaa atattaataa acaattttat agacaaatat tcgagaaaga
      841 tgaagaaaaa cgtgtaccat atagagccag atgtggaaca aatgttgctt aagtatcctt
      901 ggccaggaaa tattagagag ttggaaaata ctatagaact ggcattaaac cttcttgagg
      961 aagatggaat aatacataaa ggaataataa atagaaagat acttgaatat tttaagttaa
     1021 ataatataaa tataaagtta cttgaggaaa gtgactatga gataagtatg gaacaagaca
     1081 tacttacact tgaagagttt gaaagagcct atataaagaa agtgttaaag ttttacggaa
     1141 atgatactaa gtctaaaaaa ctggctgcca aaaaacttgg aatatcacta gctacattgt
     1201 acagaaaaat agatgtataa taattaggct ttttattctc aattctaaaa tttgaaatta
     1261 taaaattgat aaaaaacact tggaaaacta ttttctaagt gtttttttgt gtaatagcat
     1321 tttattatcc aaaaataaaa aaaatttaaa agaatttaaa aaaataaatg ctttaataaa
     1381 ttatcaaata ctaacaaata taataaaaat tatatatgta aaacaattat gttaaaaaat
     1441 tggcattata attgcttcat attaagttga gatgacaaaa taaaaaaaga aaggatgttg
     1501 tgtgagataa taataactgt cattaaaaaa tagttgggag tgataacctt gagtaaatat
     1561 caaaagaatg tttctatgtt tgatattgat ggaaggccac caattttaaa gtgtttgcca
     1621 ctatcaatgc aacatatact agcaatgata gttggaactg ttacagtacc tatagtcgta
     1681 ggaggagctg tgggtgttag ccctacacaa ataacacttt tagtgcaata tgcattaata
     1741 atagctggta tatcaacact tatacaacta tatccaatag gaaatattgg ttcaagattg
     1801 cctataattt ttggagttgg ttttacttat gtaccaacac ttgtagcagt cggaggaaat
     1861 tatggattag caagcatttt gggtgcacaa ttaatagggg gagtggctac agtattaata
     1921 gggatattca taaaaaagat aagaaaattt tttccgaata tagttgcagg aacagtggtt
     1981 tttacaattg gattatcttt gtatcctata gcaataaatt atatggcagg tggtgtaaat
     2041 agtcctactt atgcctctat gcaaaactgg gcagtagcgg ctataacatt agtggtagtt
     2101 ataggtctta gtcaatttgc aaaaggatat gcaaaattgg cagcaatatt tttgggaatt
     2161 atagttggat attgtgtatc tttagttctg ggtataataa attttgaccc tattagagag
     2221 gcaacttggt ttgcgatacc taaaccgctt gaatttggga tggaatttga tatgcctgta
     2281 ataatctcag tagtaattgt aagtataatc aatgctgttc aagcaattgg agacttatca
     2341 gctactacaa tgggaggcat ggataggaat atcacagata aggaattatc aagtggagta
     2401 ataggaagtg gaatttgtac tatgatagga gctttgtttg gaggtttacc accatcatca
     2461 tatagccaaa acgtggggat ggtagctatg aacaaagtaa taagtagatt tgtgcttgga
     2521 atagctggta tatttatgtt attgtcgggg tttgtgccta aatttggtgc attaatgact
     2581 actataccat attctgtttt aggaggagct acaatatcta tatttagtat aataacaatg
     2641 acaggtataa agttaattat tacagaagag ttgtcatcaa gaaatattac cattgttggt
     2701 ttggcaattg ccttaggcat gggaataaca tcagtcccag cagcccaaga ggcatttcct
     2761 tcatttgtaa agttagtatt tggagaatct ccaatagtaa ttgcaacatt ggttgcattc
     2821 gttttaaatc taataatacc aaataaaagt ctagaggatg aggctaaaga aagagctgtt
     2881 atggaagaga ctaattaaga tataataatt taaaagaatt gtttaaatta tttagtatga
     2941 gattgttaga ttatttttga tagctattca ttttaataaa aaatttaaca aataaatata
     3001 taccaggcaa ttaatgttca tttaagttga aaatcgggga ggcaatattt agaaatttgt
     3061 taatttttta taaaaatagc tcagtttagc tgtgtttatc ggttgagcaa taaattcatg
     3121 gtcataaaaa tatttataga aaataattat aaaaatattt agtcaagata tttatgtatg
     3181 ggaggattta ttttatgaaa acacgtaata cttcaaaata tgatgtcgat gggataccac
     3241 cactaaagga atcaatacct ttggccttac aacatctgtt agcaatgata gtaggaaata
     3301 tggtacctgc gatattaata gcaaacgtgg taggacttaa ccaggggcaa gcaacaatgt
     3361 tgattcaggg ttctatgtta gcagcaggtt tagcaacgtt tttacagtta tatccaatac
     3421 ctttatttaa aggatttaag ttaggttcaa gattacctgt aatgatggga atgagttatg
     3481 tatttttagg agcttgtctt tcagtagcag ctaaagatgg tttagcagct ctatttggag
     3541 cacagattgc tgctggggta atcgtattct ttgtaggatt tggagttaag aaaattagac
     3601 atatattcac tccaatagtt tctggaacta taatagcatg tatgggactt ggactattcg
     3661 caacagctat aaagaattta gctggaggag aaggcacaca aacatttgga agcccaataa
     3721 acttcacagt aggagttata gttgcttttg taataataat gataaataaa tacggtaaag
     3781 gtttagtaaa aaattcttca atattaatag gaatattagt aggttatgct atatctttag
     3841 ttttaggatt ggttgatttc tcagccgttc aaggagcagc tatagtatct ttaccaacac
     3901 cagcagcttt tggattagaa tttagaccag agttaatagt aatgtttact attatttaca
     3961 taataggtat agctgatatg atgggtgcct gtacaatagc tacagtagga gcaatggata
     4021 gagaggtaac tgacgaagaa ttagcatcag ttgtattagg aaattctata acttctataa
     4081 tttcatcact atttgcagct cttccaactg gagtatttag ccaaaataca gcaattgtat
     4141 ctatgaacaa agttacaagt agatttgtaa tagcactagg ggcacttgta ttattactag
     4201 cagggatatc tccagcattg ggtgcaataa tgacaactat accatcttgt gttgttggtg
     4261 gagcaacttt agtagttttc tcatcaatag caatgtctgg tttctctatt atgagtatgg
     4321 atggattcac tgaagaaaac aatcttatag caggtgtatc tatagcaaca agtatgggtc
     4381 ttacaactgc tccacaagtt ttagaccaat tcccagaaac aattggaact gtccttggag
     4441 ggtcaagtat agtttctgga gcaataatag ctatattact tcaaacatta tttaaattaa
     4501 agtcacgtaa aagtgctgaa aacgttactt caaatcttga agaaaatatt ggataaatgt
     4561 attagatata atatgagaaa accatatggt aaaatgttgt aatgaaagta ttcaaagggg
     4621 gatttaagat gattgatata ctaataaaaa atgcaataat tgttacagtt aataaagaca
     4681 gagatgttat atttgatgga gcaatagcta ttcaaggtga cagaatcttg gacataggaa
     4741 atacagttga tatcgagcct aagtatcttg atgctaaaaa agttatagat gctaatggaa
     4801 aagtaatatt ccctggcttt ataaatactc ataatcacct gttccaagtg ttattaaaag
     4861 gtttaggtga tgatatggca cttcatgagt ggttaaatac aatgatgttt ccatcagcaa
     4921 aattcttaac agaacaagat acttatgatg ctgcaatgct tggatgtatg gaaggtttaa
     4981 aaagtggtat aactacaatg gttgattata tgcatactca caatagacca ggacttacag
     5041 atggaatagt aaaggcatat aaagatttag gcataagagg ggttgtaggt agaggttgta
     5101 tagatttagg tattcacaaa gagcttatag aagatgtgga aacggttgaa aaagatttga
     5161 gaagggtatt cgaaaaatat cataattcag aaaatggaag aataaaaatc tgtgtagctc
     5221 cttcttcaat gtgggctata tctgaagaaa tgggtaagat gctttggaat atagttaaag
     5281 aatatgattc atattttaca gttcatatat ctgaaacaga atttgcaaga acagcaacaa
     5341 aggatataca tggagaaata gatataaaat tacttgaaaa atggggtata gtaggtccag
     5401 aagttgtagc agtacattgt gtatgtataa ctgatgaaga catggaaatg cttaaaaagt
     5461 atgacataaa agtttctcat aatgtagcaa gtaatatgta tcttgcttct ggtgttgcac
     5521 ctgtgccaga aatgttaaaa aaaggtataa atgtgagttt aggtcttgac ggtgcagcaa
     5581 gtaataacgc acaagatatg gtagagctta tgaaactaac agctttacaa cacaaagtaa
     5641 ataattgtga ccctctggca atttctgcag agaaagttct tgaaatggct acaattgagg
     5701 gagcaagaac tcttagaatg gaagatgaaa taggttcttt ggagattggg aaaaaagctg
     5761 acctagtaat atttaactca atgctttctc caaaatcaat acctcttcac aatcctgtat
     5821 caactttggt atactcagct agtatgcaca atgtagagag tgttttagtt gatggaaatg
     5881 taatacttga aaatggaaaa gtaactacaa ttgaaagtga agaacaagtg ttgcttaatg
     5941 cacaacatgc agcagaagaa ttatgtaaga gagctaatgt aactaataga atggaaggac
     6001 ataagtggaa tagcttatac taatattaaa ataagattgt aataatattc tattacaaca
     6061 ttagtttatc tgtaggagga gaaaatggaa aaaataaaat taaatattaa tgattacatg
     6121 tatgaaatag aagttgaaaa gaatgaaact cttttgcatg tacttcgtga aaaattaaat
     6181 ctaactggag cgaaacaagg ttgtaatagt ggcagttgtg gagcatgtaa agttttgata
     6241 gatggagttg acatgaaatc ttgtaaactt cttgcaaaaa actgcgaagg taaagttata
     6301 tacactatag aaagttttgc taatggagaa aatcttcatc caatacaaga agcttttata
     6361 gaagttggag ctgtacaatg tggatattgt actcctggta tgattataac aacacaagct
     6421 cttttaaata gaaatgcatc tcctaatgaa gatgaaatta gagaagcttt taaagaaaat
     6481 ctctgtagat gcacaggata tgtaaagatt atagaagctg taaaattatc tgcagctaga
     6541 ttagggggta gataattatg gaaaaggtaa taggaaaatc ataccctcta aaagatgcag
     6601 ctttaaaagt tactggtcaa atgaaatacg tggctgatat gaaaccacaa aatgtacttt
     6661 acgctaagat gcttttaagc cctgtagcac atgcaaaaat aaaaagtata gatacaagtg
     6721 aagctgagaa agtagtaggt gttagagcta tagctactta tctcaataca tcacaaagaa
     6781 aatacaatgg ttcattaaga ttttatgagc acaaaatacc tgaaaatgaa gttatatttt
     6841 cagatacagt tagatttgtt ggtgaccgtg ttgcagcagt agctgcagag actccagaaa
     6901 tagcagagaa agctgtaaaa cttataaaag ttgaatatga agaattgcca gctgtattta
     6961 ctatacaaga tgcaataaaa gaagatgcta tctgcataca tggagattca aatatagttt
     7021 ctcaaaacaa cataaatggg ggagatgtag aaaaaggctt caaggaatgt gactatatat
     7081 ttgaagatga atactctact ccagcaatac atcatagtgc aatagagact catagtgtaa
     7141 ttgcacacta tgattataat gataaattaa ctgtacatac accttgccaa aatacatttg
     7201 gatttagaat aatactatct caaatttttg ggatgtcatt aaataaagta agagttataa
     7261 gacctgctat tggaggctca tttggtggaa aattagaact tacaattgaa ccagtagcag
     7321 ccgcattatc aaaaatgaca agaagaccag ttaaaatggt tcttacaaga aaggaatcta
     7381 tgatatcaac aagaactcgt catgggtctt ttgtaaaatt aaaaactggt gtaaataaag
     7441 atggtgaagt aatagcacaa gacataaaaa tatatacaaa tacaggagca tatgcatcaa
     7501 gtgctttaaa tgttatagga gcacttagtc ataaagtatt taaagtttat aaaataccaa
     7561 atataaagtt tactgggatg cctgtatata caaatacacc aatagcagga gctatgagag
     7621 gctatgggtc acctcaaata tttatggctc aacaagctca atttgcgaaa atagcaaagg
     7681 aaataggtat ggatttagtc gattttcaaa ataaaaatgc agtagagcca gatgatgtcg
     7741 atataatctt ccacggttct cttggaaatc ctagagtatt ggattgtata gagcaaggta
     7801 agaagatgtt taagtgggat gaaaagaaaa aacttccaaa agaagaagga aaatatttaa
     7861 gaggtatagg tatggcaata ggtgcacatg gtaatggagt ttttggtgca catagagatg
     7921 ttgttacatt gactcttaaa ttaaatgaag atggtacgtt gacactgcta acaggagctc
     7981 atgatatggg taatggtgtt gtaactatgc aaacaatgat ggtagctgaa gttttaggaa
     8041 taacacctga taaagttgat acatttgaaa ctgatacgga tgcatgtaca ttcaaccttg
     8101 gtgactatgc aagtagaggg gtatttgtag aaggtggagg agctaaaaag acagcagaga
     8161 aattgaaaaa tcttatatta gaagaagcag aaaaattact tgaaacatca aaagaagatt
     8221 tatatctaga caatggatgt gtaattagta aacttgatga aaatgtaaaa gcatcacttt
     8281 ctgatgtcgc agtatatagt caaagtaaat cattaagaga gttaactgtt gttgaagatt
     8341 attcttcacc agctggacgt acttcttatg gtgtacattt tgctgaagtt ttagtagata
     8401 aagaaactaa ggatataaaa gtagttgatt ttgtagctgt acatgatgtt ggtagagtta
     8461 taaatcctct aaatttagaa ggacaattag aaggtgggat tcatatgggt ctaggatacg
     8521 cattgagtga agaattggca tttgatgaac aaggtagacc taaagctact gattttaaaa
     8581 catataaatt atttagaact gtagatatgc caaaatgtca agttgcattt gtagatggtt
     8641 atgaaaaagc tgggcctttt ggtggtaaga gtattggaga atgtgccgtt gtaccagttg
     8701 cgccagctgt tgcaaatgca gtatataatg caactgatgt agagatacat tcattaccaa
     8761 taaaattaaa atagtataaa ttaaagcaga aataacaatt tattgtattt gtaaatagat
     8821 aataaaaata gatttatttg gtgaggagat attattatgg caaagatatt aggtatttgt
     8881 ggaagtccac gtaaaggagc aacagaatat gcagtgttag aagctttgaa agaagcagaa
     8941 actatacctg gtatagaaac tgagtattgg tcagttagag ggaaaaaaat aagtccttgt
     9001 gtacattgtg atgcatgtat aagaaaagaa actatgtgta ttataaaaga tgatatccaa
     9061 gaattagagc aaaaaatatt ggaggcagat ggaattatag taggttctcc agtttatgat
     9121 atgaatataa ctgctcaact tacagcagta tttaatagac ttcgtccaat gtatttagtt
     9181 catccaggaa aacttcaaaa taaggttggt gcagctataa ctactggtgg aactagatat
     9241 ggtggccaag aattgacaaa acttcctata ataaatttct tcctaatgca tgaaatgtta
     9301 gtgtctggag gtttaggtgg atgctatata ggtggtacaa tatggagtaa agatggaaaa
     9361 gcaaaaggtg cccaagagga tgagacaggt atggacacag taaaaagact tggaaaaggt
     9421 gcagcagagg cagttatggt ttctaagttt ggtcttgaaa aatggaatgt cgtaaaagaa
     9481 gaattagatg ttaaaaaaga tgaaaaatca cctcttagag accactaaag atttattata
     9541 ttattgaata tatacaaata gtgatgtaat agattattgc atcactattt tatgttttaa
     9601 tttagatttt atagatttta tattaaaaaa tatagaaaca aagtgttttg agatttattt
     9661 ttaaatttta aaaatgagaa ttaaaaagtg agaagataga aaaggttttt tagttattaa
     9721 attctacata cattgtatga actaaagata gaaaagaaaa aataataaaa gcattgcaat
     9781 ttctatacta aagagtcata gaaaaatatc tagtatatat ttagtgttgg catgttaatt
     9841 gcttatatat taaagtataa aaatttaaca ataatttgga ggtattttag atgttaaaag
     9901 aaattaaatg gaaagtaaat aatttgccaa agggcgataa ggaaaattgt atcaaatttt
     9961 taaatgaaga agaaataact aaggttagaa attttcacaa aagctttcct caatataaag
    10021 aaactccatt agcaaaccta gaaggtttag caaaaaaatt aggtgttgca ggagtatatg
    10081 taaaagatga atcttataga tttggattaa atgcatttaa agtattaggt ggttcttatt
    10141 ctatgggtag atacttagct caaagattag atacagatat atctgagtta gggtatgaca
    10201 aattaacttc aaaagaaata aaagaaaaat taggagaaat aactttcttt acagctacag
    10261 atggaaatca cggtagagga gttgcttgga cagctaataa attaggtcaa aaatctgttg
    10321 tattaatgcc aaaaggttct tctgaattta gattaaataa aattaaagga gaaggtgcag
    10381 atgctagtat aacagactta aactatgatg atgcagtaag attagctaat gattatgcag
    10441 aagctgatga ccatggagta atggttcaag atacagcatg ggacggatat gaagaaatac
    10501 cagcgtggat aatgcaagga tatggaacta tggctcaaga agctatagag caattaaaag
    10561 aatatggagt agatagacca acacacgtat ttgttcaagc aggagttggt tcacttgctg
    10621 gtgcagttca agggtatgtt gcttcaatat atgatgaatg cccaataaca gtagtagtag
    10681 aggctgatga agctgattgt tactataaat cagcagaagc tggagatgga aaaccaagat
    10741 ttgttggagg agatatgcca actataatgg cagggcttgc ttgtggagag ccaaatacta
    10801 taggatttga agtattaaaa aatcatgcag cagcatttgt ttcagcacca gactgggttt
    10861 ctgctaaggg tatgagaaca ttaggtaatc ctttaaatgg tgatgaaaag gtaatttctg
    10921 gagaatctgg agcagttaca actggtcttt tagtagcagc tatggaaaga gaagatttag
    10981 cagatttaag aaaagattta aaattagatg aaaattcaag aatactttta ataagcacag
    11041 aaggagatac tgaccctgac aaatatagaa gtatagtttg ggatggagaa tatccaagta
    11101 tataaggaat tactaaaatg caattattag atattggttt acatatttag aagatatata
    11161 agttctaaaa tttagaaaat atataagtat ataaaataca tacattataa aaaatctagg
    11221 gggataatga aatgttaaac gaacaaagaa aacaagaagt aactgaaata tgccaaaaat
    11281 taattcaaaa tcctagttct tctggaaatg aagaaggtgt tgtaaaagca atagaagagt
    11341 cttttgaaaa attaggtttt gatagttggt caagagatag atatggaaat atagtaggat
    11401 gtataaaagg taacaagcca ggtaagaaaa tattatttga tggacatata gatacagttc
    11461 cagttccaga tgcaagtaaa tggagtgttc ctccttttga aggaaaaata gttgatggaa
    11521 agatatatgg tagaggaact tctgacatga aaggtcaata tactgcaatg atgtctgctg
    11581 tagcgtattt tgctaaagac actaatagag attttgctgg agaactatat gtagcaggtg
    11641 ttgttcatga agaaatattt gaaggtgttt cggctagaga gataagtaag gctgttcaac
    11701 ctgactatgt tgtaataggt gagtcttcag agttaaactt aaagattggt cagagaggta
    11761 gaggagaaat agtagtagaa acttttggta aaccagctca ctctgctaat ccagaaaaag
    11821 gt
//
LOCUS       DAMKMI010000119        11768 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMKMI010000119 DAMKMI010000000
VERSION     DAMKMI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11768
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.124"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            52..522
                     /gene="trmL"
                     /locus_tag="PC429_002913"
     CDS             52..522
                     /gene="trmL"
                     /locus_tag="PC429_002913"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF3848402.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            558..1406
                     /locus_tag="PC429_002914"
     CDS             558..1406
                     /locus_tag="PC429_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF3848403.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            1728..2732
                     /locus_tag="PC429_002915"
     CDS             1728..2732
                     /locus_tag="PC429_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3848404.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            2858..3970
                     /locus_tag="PC429_002916"
     CDS             2858..3970
                     /locus_tag="PC429_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3848405.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATEYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            4460..5221
                     /locus_tag="PC429_002917"
     CDS             4460..5221
                     /locus_tag="PC429_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848406.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            5224..6042
                     /locus_tag="PC429_002918"
     CDS             5224..6042
                     /locus_tag="PC429_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF3848407.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            6222..6824
                     /locus_tag="PC429_002919"
     CDS             6222..6824
                     /locus_tag="PC429_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF3848408.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    7283..7520
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7584..9503
                     /gene="thrS"
                     /locus_tag="PC429_002920"
     CDS             7584..9503
                     /gene="thrS"
                     /locus_tag="PC429_002920"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF3848409.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            10271..11362
                     /locus_tag="PC429_002921"
     CDS             10271..11362
                     /locus_tag="PC429_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF3848410.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
BASE COUNT         4760 a         1125 c         2122 g         3761 t
ORIGIN      
        1 gtttataaaa taaaatttga taaataaaag cgatacttag ggaggattaa aatgtcatta
       61 aatatagtat tagtagaacc agaaatacct caaaatacag gaaatataat aagaacctgt
      121 gcagcaacag gttcaacttt acatttagta aaaccactgg gtttttcatt ggatgataaa
      181 cacctaaaaa gatgtgggct tgactattgg gatatagctg atatacaata ttacgatagc
      241 tttgaagaat tacaagaaaa gtatccaaat agtaaatact ttttttctac aacaaaggct
      301 aaacagtcac attcagatgt gaaatatgaa gaaaattgtt ttatagtatt tgggaaagaa
      361 acaaagggat taccagaacc tttgttgaaa gcaaatatgg atacttgtat tagagtgcct
      421 atgttggatg ttgaaaaagc taggtcatta aatttatcaa attcagttgc tattgtagtg
      481 tacgaggctt taagacaaat aggataccca aatatgaggt agtttcaaca agcctaaatg
      541 agaaaggagc atacttaatg aaaaatctaa agataatctg tgacagttta tcggacgtgc
      601 caaaagattt gttagataaa tatgatatag atatagtttc attaacagtt atactggagg
      661 aaagagaata caaggataga attgatatat ctggagatga attttataaa aagcttagag
      721 aagaaaatgt atatccaaaa acttctcaag ctacttatgc ccaatttaag gaaatttttg
      781 agaaatatac aaatgaggat agagagatac tttatatagc agggtctgcc acagctacag
      841 gtacgtatca aagtgctgta atggcaaaga atgatgtaga tggtaaaata tatacatatg
      901 actctaactt gctttgtatg ggaactggtt tacttgtaat aaaagcagga gaacttgcaa
      961 gtgaaggaaa gtctgttgct gaaatattgc ctgttttgga tgaattaaaa gagaaggctt
     1021 ttgttatatt ttcagtagat acattggaat atttacaaaa gggaggtaga atatcatcta
     1081 ctaaggcagc aataggaagt atattaaata taaaaccgat tcttgaaata agagatggat
     1141 tagtttcgca aatatctcaa gtaagaggaa agaagaatgt aatttctaag atgatggaat
     1201 tgataaaatc aaattgtggt gatgatttat cagaccaaat tgtatatata ggatatagtg
     1261 atgattttaa ggaaaaagaa caattatcta aggttatgca agatgaatta agacctaaag
     1321 aaattagatt tttccaaatt ggtgtgtgta taggtgcaca tgctggtcct ggtgttactg
     1381 gtatcatatg cttaaaaaat aaataatata tttttggatg acattattta tttttttatt
     1441 aaattaggat agaatattaa ttattgagat gttaaattgg atttattttg gatgaaatta
     1501 agtgttgata tattagattt agtttgtagg gaagtgtgta aatagataag aatctattgc
     1561 acactttttt atagtaagat ttattaaaaa aatttcaaaa tgtacttgta aattgtatat
     1621 aaaaaaatta taaataggat gtaaaagtaa ataggatgta aaagtaaata ggatgtaaaa
     1681 gtaaatagga tataaaaata tcgtaaatgt atgaggagtg aaatttaatg cgtttcaaaa
     1741 gaatattgat attgatgtta actgtagcaa tgatagctgg aatgttggtt gggtgtgcta
     1801 ataacccaag caataatcct agctctagca aagataataa aagtgatata agcgataaaa
     1861 gtaattcaaa aaaatctgta tcaatgatac tagatattga aggaacaaat aatgaagcta
     1921 tgaataattc agctttatta gcattaaaca atgctcaaaa gaaacttaat atagatacaa
     1981 acaaagtgga atcagatgat tcctcaactt tttctaatag tatagatatt ttatgtaatg
     2041 acaattacga tttaattata gctgtaggag ctagatttgc aaaaccattg gagatggtag
     2101 ccaagaaata tccaaagcaa caatttgcaa ttatagacta tgagtatgac aagcaacctt
     2161 ctaatataac ttcaatttct tatgaagata ataaatctgg atatctagct ggattaattg
     2221 ctggaaaaat gactgaaagc gataaggttg gttttatagg aggaacaaag agttctagta
     2281 gagataaatt tgaatcaggt tttagagagg gtgttaaatt ttctaatagt agtataaagg
     2341 atatttcagt tgagtatgca gatgttttta aagatagcaa atcagtagaa tctatagcta
     2401 aaaaaatgat ggataatgga gtggatatta ttttttctac tactgaagat gattcaaaag
     2461 ctgtaataga tgcagtaagg gcaaaaaata aaaaagttat agcaacgaat aaagaccaac
     2521 atgagttagc tccagaaaat gttataagtt ctattgtaaa agattttgag aatcctactt
     2581 ataatctgat acaaagtttt gtaaaaggaa attataaagg cggaaaagtt atagaaaaca
     2641 cagttaagtc tggaagtact gggcttgccc aaaatagttc tcaaaatatt cctccagatg
     2701 tgttagaata tgttaataag aacaataaat aatctactat aatatccaag aatacttatg
     2761 actttagtta tgaggtgaat tggatgttat aatagacata tatagaaaga catatgcaga
     2821 ggtaggtcag acctatctaa atcagggagt gaattttgtg aaaataaaaa ggatattgat
     2881 ggcttgcttt gtagtagttt ttacaatttt tatattagct atatgtaatt ttgattttgg
     2941 gtttgataaa gtaaataaga ataattctaa tatagatagt gctaaatcga aacagagtga
     3001 gtctaaaagt ttgccaactt cagcagaaaa ggataaatca aatagtaaaa gtaagaataa
     3061 agaagatgac acagaagatg attcatatgt aagaaaaact attggagtat tgttagatac
     3121 cgaaaactta aataacgaat catataataa caatgtacta tcagccttaa aaagggctga
     3181 cgaagatttt gatgttagga taaaacttga aaaaccaaag acttctaaaa gttttaaaga
     3241 gagtatagaa aaactgcata agtatgaacc agatttgata atagcagttg gagccagatt
     3301 tgcagagcca attaaagaag ctgctactga atatcccaaa cagcaatttg caatagttga
     3361 ctatacttat aaagttcagc cagctaatgt aatttctgta tcatttcaag ataatgttac
     3421 tggatattta gcaggactta ttgcaggtaa aatgactgat actgggaagg ttggttttat
     3481 aggaggagtt gagggttcta gtagagataa gtatgaatct ggatttagaa aggggcttaa
     3541 aacttcaaat aaagatgctg agttactagt taagtattca aatatatttg aaaaccctga
     3601 atcagctaga actattgcaa aggaaatgag agattctgat agagatgttg tattttcagc
     3661 tacagaggat gatagtaaaa gagctattca ggtagcaaga gaaaatggag gtaaagttat
     3721 agctattaat aaagaccaat acgatatggc tccagataat gtaataagtt caattataaa
     3781 agatgtagaa caacctgtat atgaactgat aaagaacgtt gcaaaagatg gttttaaggg
     3841 aagtaaagta atggaattta aaataaaaga tggagaactt ggcttgactt caaagagcag
     3901 tagaaatata ccaccagatg ttttggaata cataaaaaaa gagtataaca aagtgaaaga
     3961 tacatattaa ttattgatgt agaagattat aaaaatatac tgcttaagtt tagattggtg
     4021 ataaaaggat atatttatta aatttgtgct aactgtacaa aatatattga ttaagtttag
     4081 attagtgata aaaagatata tttattaaat ttgtgttaac agtatggaat atactgctta
     4141 agtttagatt ggtgataaaa ggatatattt attaaattta gtctaactgt acaaaatata
     4201 ttgattgagt ttagattagc aataaaaaga tatatttatt aaatttgtgc taactgtacg
     4261 gaatatactg cttaagttta gattagtgat aaaaagatat atttattaaa tttgaaccaa
     4321 ctgtacaaag ctatactaat taagcttaga ttaactatat aaaattataa caataagtgt
     4381 gtgggcagaa aaattctgct acacacttta tttatattca cataaaaaat ttatcaaaaa
     4441 aaagaaaaag gagtaaaaaa tggaatttac aagagaacca tttatactag atgacgattt
     4501 gtgtctagct ttggtggaca aaagaattag ttatctaatg gaaaatgaat taaaattaag
     4561 aaatatagag ataataaaaa caatagaatg taaagaatta tatgaaagca taaaatatca
     4621 cccggatata tgtatttgta atttaggaaa aggagatata attgttgcgc cgaatgtata
     4681 taatcaatac aaagagttat taagtagata taaatttaat ataattaagg gagaagctaa
     4741 tttattaaat agataccctt atgatatacc ttacaatatt gcgatagttg gtgactatgc
     4801 tatacacaat tttaagtata ctgacaaagc aatattagat tatattgaaa ataataaact
     4861 taaaaagata aatatagagc agggctattc taaatgctca atctgtatag tagatagaac
     4921 gtctataata acatcagata aaggtatttg gaaaagtatg aagaatacag aaatagagtg
     4981 tttattgata gaaaaaggtc acataaattt atttgagatg aattatggat ttataggtgg
     5041 atgtacaggg ttgatttcta aggataaact agctttttgt ggagatgtta aaaaacatcc
     5101 tgactatgaa cgtataaaaa gttttgtaga aagtaaaaac aaggagatag ttacacttag
     5161 ctgtgaaaat ttactagata taggttctat agttcctctc atgacaagaa aggagcgata
     5221 actttggttg agaaggataa tatttcttcc atgaaatttt tgaggaataa taatgtaaac
     5281 gatagattaa tatatacgaa tgacattgta gaagtagtga tagattctgc aaagcaagaa
     5341 gtgttaaata aaaaaatact taatggtact aaagctgaac aaagagcttt aaatattgat
     5401 gagatatcaa cggaaataac tagtatgata ataagtataa tgaaagagaa acttttgaaa
     5461 gaatacattc taaaatgcta tgggcgtaca tatcctgaag ataaaaataa tatatacata
     5521 tattcactga acatatttgc taaaaaagag atttttatga gagaaacagt atttactagg
     5581 gtgagaaatt atatagaaga aaatgatttt ataaatgtag aaggttttat tagatttaga
     5641 atgagagaat ttatgaaata tatttctgct ataggtgata ttgcagttga ggagtattta
     5701 ataaagaaag accaagatga atttataaga gttttaaaat actttataga tactcaagaa
     5761 gaaaagattg atttgcttaa agttcatata atggaagata atacatttgt tttatacgat
     5821 aaaaatggaa ataagataga cagtatagat gatgaagaaa ttataaatat ggtaataaga
     5881 gaaaatttaa attatgagga ctttttaata agtaccttat taactctatg tcccaaaaaa
     5941 atagaaatat tggatttgtt aaataataat tgctcaaagg aaattataga tactgtagaa
     6001 gcaatttttg aaaataaagt tagtataatt atggaaaact agaatctaag catataaaag
     6061 ctatccatat aaaattaaaa aaattctttt taaatcgaca caaaagactt ataattgtat
     6121 ataaagctat agtataacta gtgttattaa gtataaagaa tatctacttg attaatacaa
     6181 tatagctggc aaatatacaa ttgataagga gtaattttat aatggataat ttaattagga
     6241 tactgatatt agctgttatt ggtggattta taggttatgt tacgaatgta gttgctataa
     6301 gactgatatt tagaccaata gagcctatta aaatacctat tttaaatata gaaatagtag
     6361 gtcttatacc aaagagaaga gcagaaatag cagcaaatgt aggagaaata attcaagaag
     6421 aatttctttc tatggacgaa atacttgcaa atatcattac tgacgaagat aaggaagaag
     6481 ttgtaagata cataaaagca agggtaaaaa ttataataca tgagaaagtt tcatttatcc
     6541 cgagtggtat aaaaaacatg atacaagatt atctaggaga gataattgag tctgaagtta
     6601 agcaaagtat agatgaattg agtaaaaata taataaataa agcaaatgaa cgtatagata
     6661 tacaaaaaat ggtcgaagat aagattaatg aactagattt atatgaattg gaagagataa
     6721 taataagaat agctaagaaa gaattaaaac atatagagtt tttggggctt gtcttagggt
     6781 ttttaatagg tatagctcaa gggttaatta ctatgtttat ttaaatatat gagaagtagc
     6841 atttatttaa gtactaatta tatttaccta tttgagtgaa ttacttatta atagcattta
     6901 gatattaatt gtatttattt atttgattgt gttacttatt aatagtattt aagtatttac
     6961 tgtactactt attaactata ttaagtattg attgtattta agtatacaaa acctaaaaag
     7021 agtacaaaaa ttagaagtta gttttaataa aaattagttt tagtatttat ctagtcatat
     7081 ctatcatgca tgtggatacg ttttaaaaat tttaaattta tgaaaatatt ataatatcac
     7141 tataaatcaa gagattaatt taatataaaa atataagaat attggtttac agctgattga
     7201 gatgaaaaat aatgggtaat taatatatta cattaatgtt gattgatgta taactatctg
     7261 ttataatgaa aatatattaa aaatcatagt gttgaagagg aagagtagag tagaaaccat
     7321 tctcagagaa tttgggtcta tggctgagag cccatgtaat aggtctattt gaaaactacc
     7381 ttggagctga gcttattagc agtatttgtt ttgtactata cgtgaaagtg ctcgggtgac
     7441 gaccttatag tcatcaagag ggttgtatat acttgtatac gccaacttgg gtggaaccgc
     7501 gaattaatat cgtcccaaac atatttgggg cgatttttta ttacaaaaaa tctaaaaatt
     7561 atttcaataa ggaggaaaaa acaatgatta aagttgcttt aaaagatggc tcaataaaag
     7621 aatttgaaaa tgctatttct gttatggatg tagctaaatc cattagtgaa ggattagcta
     7681 gaaatgtggt agcagcgtct gtgaacggag aggttgtagg tctggaccac attatagata
     7741 ctgattgtga cttgaattta tttaagtttg aagataaaga aggtaaggaa gtttttagac
     7801 atacatcagc acatatatta gctcaagcta taaaaagact ttacccagaa gcaaagcttg
     7861 ctataggacc aagtatagaa aatggattct actatgatat agatttagac catagattag
     7921 ttccagagga cctagagaaa atagaagctg aaatgaaaaa aatagctaaa gaagatttaa
     7981 aaatagaaag atttgaactt cctagaaatg aagctttaga gcttatgaaa gaacaaggag
     8041 aagactataa ggtagaactt atatctgatt tacctgaaag tgaaataata tctttctaca
     8101 agcaaggtga ttttactgat ttatgtagag gaccacattt accatctact aaaaaggtaa
     8161 aagctgttaa attacaaagt gtagcaggtg catattggcg tggtgacgaa aataataaga
     8221 tgcttcaaag aatatatgga acttcttttg agaaaaacaa agacttagaa gagtacttac
     8281 atttactaga agaagctaaa aagagagacc acagaaaatt aggtaaagaa ttaggattat
     8341 ttatgatacc tgaagaaggt ccaggattcc ctatgttttt accaaaaggt atggaactta
     8401 aaaatgaatt attaaaattc tggagagaaa tacacagaaa agctggatat attgagatag
     8461 aatctccaat catattaaat agaaaattat gggagacttc aggacactgg tatcattaca
     8521 aagaaaatat gtatacagtt aaaatagatg atgaggatta tgctataaaa cctatgaact
     8581 gtcctggtgg attaatttat tataactctc aactacattc atatagagat ttcccaatga
     8641 gagttgcaga gcttggtaga gttcatagac atgagttatc tggtgcatta caaggattaa
     8701 tgagagttag agcatttact caagacgatt cacatatatt catgttacca gagcaaatta
     8761 aggacgaaat aaaaggagta gctaacttaa tagatggtat atacaaaact tttggatttg
     8821 aatataattt agaattatct acaagaccag agaactctat gggaagcgac gaagagtggg
     8881 aagcagcaga aaatggatta agagaagctt tagaagagtt aggtctacca tatactatta
     8941 atgagggtga tggagcattc tatggtccta agatagactt ccatctaaaa gattgtttag
     9001 gaagaacttg gcaatgtggt actatccaat tagatatgca gcttccaaga caatttgata
     9061 atacttatat tggtcaagat ggtgaaaagc atagaccagt tatgattcat agggttgctt
     9121 ttggtagtat agaaagattt ataggtatat taatagagca ttatgctggt aaattcccag
     9181 tttggttatc tccaactcaa gttaagatac ttcctatatc tgataaattt atggattatg
     9241 caaatgaagt taagaaagag ttatttgata agggtataag agttgaatta gatgatagag
     9301 ctgaaaaaat tggatttaag ataagagaag ctcagcttga aaaagtgcca tacatgctaa
     9361 tagtaggtga aaaagaagta gctgataata atgtttctgt tagatcaaga gataaaggtg
     9421 aaataggtag tattaaatta gatgaattta tagctagtat ttcaaaagaa attgaatcaa
     9481 gagaaagtat tattcaagac taatatatat tatattagta gctaagatat tgttatataa
     9541 gataagttta atcaatatgt tgatatgttg atgcatagtg attaaattta tgaaaagatg
     9601 agtataatta gaggtacagt taatcatatt tttatgatta atgtacctct agttttttgt
     9661 atggtagatt tattaataaa tatatcatag aagtgttttg aacatggaaa tatttatgtg
     9721 cattctttaa ataaacgttt attttttgag tgataattaa gtgcttgttg gtaaggttat
     9781 ggctgtaaaa atcataactt tattttcata tttgttccat tttcataaaa attattatcc
     9841 ctttaagaaa atttcttaat cctcttgaaa tatttccttt ttagtctaaa gttaaatttc
     9901 tttcttcaaa atgaatattt aaatgaacta aaatttaagt acattttcta atcttatgag
     9961 tctatttttc atagataatt gccctataat agaagaatct atgttggtgc tatgtggtga
    10021 caataacagc attgtcagtg attaattaat gctttataaa tgattaatta gtgttttatg
    10081 ttatttttgg acttaccctc tatacataga ttaatttatt taactttaga tagagagaga
    10141 atgtcaagta catacacaga cattagagtt taagaaagtg tttatggagc aagaactttt
    10201 aaaagaggta gtgacttagt ttaatttcaa gaaatgataa atacaaaaga ggaaaaagag
    10261 aggtgttgaa atgatagaaa aagtagaaga aaaatctaag attattaaaa catcaaaaga
    10321 atataagcga actataattg aagaaaaaga taaagaatgg accaaaaaat taaatgatat
    10381 acttagtaga agtagttttt ctaatgatga attagataaa atcaaggcgt taattccaaa
    10441 agagattttg actaatgaaa atgttggaga agttgtgact gaagatggat atgctaagcc
    10501 cgagtatgat gaggtattta aatcattgtt cactttaaat aatgaatatg atttacttgt
    10561 aaattttata aatgatattc taaaagatgc tccatatgct aacaggaata tcaaaccgtt
    10621 cactcaaata aagagaataa ttagagttga aactgatcca acaattaatt acattggaga
    10681 gaaaagacct aaacttgata ttttggctga agatgaagaa aataatcata taaatattga
    10741 gatgcaaaga gcttttgaga acgattattt ggaaagagct gaatattatc ttagtcgtgt
    10801 acatggtaga aagttagaag aaggtaaaga gtataaagaa attggaaaaa ctattggaat
    10861 acatatacta aatcatgtaa aatataatca catagaagat tatgtaaatt gcctgagatt
    10921 aacaatggac gggcatccag atatttattc tagtaaaact gcattatatt ttatagagct
    10981 tccaaaaatt cataaatctg actatatagt aaatagaatt atgctatggg gaaaacttat
    11041 tagtaatcct tctaacttag atgtaagagt tatagctaag aatgattcta ttattaaaaa
    11101 agcacttgat agattaaaag aattaggtag caatgaagaa tatttattaa atctgaaaag
    11161 aggtgcttat atcatgaata aaagtagaaa ttttaaagaa gagattgaaa gagagacagc
    11221 aattagagtt gcaaaagaaa aagattataa aataattata atgttgaaaa aaaatggttt
    11281 taaattagat gacatattaa ataaatttaa tgattttgac ttaagtgaag atgagataaa
    11341 agaagtatat gaaaataatt gatgataaat aaaaagtgaa cttatataag tttgctgaat
    11401 cggagtctgg aaatatttat gtgtattttt taatgaatga aaatttattt taaaaacaat
    11461 aatttagaag tacagttaat tatattttta tgatactgta cttctagttt tttctattgt
    11521 agatttatta tttactaata aatctacaat agaaatgctt ttgttgtaag aatcatttta
    11581 tctaatgatt gttttttata atagttaaat ttccatttgt agtgtttaaa tataaatcta
    11641 taacagcatt tctataaatg ttataaatca ctataatgat tcaactcaaa gttactgctt
    11701 aaagaagcag agattttatc aaggtatatc atggaaaaaa atattttatt gtaatattta
    11761 taagattt
//
LOCUS       DAMKMI010000120        11680 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMKMI010000120 DAMKMI010000000
VERSION     DAMKMI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.125"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            305..1483
                     /locus_tag="PC429_002922"
     CDS             305..1483
                     /locus_tag="PC429_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3848411.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            1833..2714
                     /locus_tag="PC429_002923"
     CDS             1833..2714
                     /locus_tag="PC429_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3848412.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKISNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            2905..3072
                     /locus_tag="PC429_002924"
                     /pseudo
     CDS             2905..3072
                     /locus_tag="PC429_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(3332..5899)
                     /locus_tag="PC429_002925"
     CDS             complement(3332..5899)
                     /locus_tag="PC429_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF3848413.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(5902..6507)
                     /locus_tag="PC429_002926"
     CDS             complement(5902..6507)
                     /locus_tag="PC429_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3848414.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(6881..8341)
                     /locus_tag="PC429_002927"
     CDS             complement(6881..8341)
                     /locus_tag="PC429_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF3848415.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            8695..9273
                     /locus_tag="PC429_002928"
     CDS             8695..9273
                     /locus_tag="PC429_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3848416.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            9324..9557
                     /locus_tag="PC429_002929"
     CDS             9324..9557
                     /locus_tag="PC429_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848417.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            9566..10816
                     /locus_tag="PC429_002930"
     CDS             9566..10816
                     /locus_tag="PC429_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3848418.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            10977..11546
                     /locus_tag="PC429_002931"
     CDS             10977..11546
                     /locus_tag="PC429_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF3848419.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         4027 a         1933 c         1655 g         4065 t
ORIGIN      
        1 ctatatataa ttactatact tggacacata gtcatcatac atgctatatg taattactat
       61 acttatacac ataactatta tactttccat acataattac tatatttgga cacatagtta
      121 tcatacatac tgtatattaa gatataatac tttttaaatt ttatataaca cagacacatg
      181 caataaaaaa gaaggtatcc ataataagct ctttgcatgt acctaaataa aaactgtaaa
      241 atagaatata taaaatctaa tctattttaa gtaaatacaa tttcacttta aaagaggatg
      301 tactatgaaa taccatatat ttttaatcat aactactata ttaataatcg aactatgtaa
      361 catttacata tgcaaaatag catcttttag aaacaaaaat ataactatac ataagaatta
      421 caaatatatt tttttaataa aaccattaac cttcttgata ataatattca caaaattacc
      481 attaccacta tattatatat tgttttacat agtaagtcat aaattgctct taagcagaaa
      541 aagttctact aacaaccatt ttattcccaa tatggcagta ataagttttt gtattttaca
      601 ccttattact gtaagtacaa tatcacttgt taaaaataca agtccagata taatatttgc
      661 aaatatgacc tataagttaa ttagtatttt aatagcttct attttagatt ttatcttatg
      721 gtactctatg aataaaataa aagagcatat ttatataata actcaaaatc aaatagagtt
      781 cagtctattt ttaaaattta tctggttcac tttaggatat atattatttg atagtataca
      841 aattacattt gctccaccaa gttctctaac ttcctacttt atattgggaa gtaacatatt
      901 attaatctta caactaatta tattcatcaa tcatatatat tccattgaaa aaaatattta
      961 tttggaaaaa gaacataata gactaaaaat gataaaacaa gaacaattac taaaagtaaa
     1021 tttattaaag aaaaaagcca acattgatga attaacatca gcatataatc gaaaatttgc
     1081 ttttgaaaaa atacagcatt acattgataa caatttatta ttttcattga tatatatcga
     1141 tttggataaa ttaaagacga taaatgatac atatggacat ttagctgggg ataactatat
     1201 tattaacttc tcaagtatag ttattgaaaa cttaggaata gatgacatat ttgctagaat
     1261 aggaggagat gagtttatag ttattatgcc taaatctaat gaaaacacca caaaagaatt
     1321 tatagagaaa ataagtaaaa ttatggataa tacgatatct tttagctatg gtattgtaga
     1381 agtttctagc gaaaacaaaa tgactgtaga agaaattatt aatattgctg atattagaat
     1441 gtacactaat aaacaaaata atcataaaag gaacaataac taaaatgaat atttacatga
     1501 gcttacttgt agtctcacta tgttttttat cttattatct ctatcattat aattttttta
     1561 aatatctgga cataatgatt aataactcta aaaataaata cacagtatat attatatatt
     1621 ctatatttaa ttatttaatt tttattattt gtacttattt aaagtttcat ctaattataa
     1681 attggacaat ttttatgttg attttattca tacaaattaa agtaacttat aaaatctcca
     1741 caataaaaaa cattttttat tcactaaact gctccatcat tggtttagca ataaatatat
     1801 tttttaggtc tttaatgtca atagtgttaa atatgccagc atatttattt gatagtaatt
     1861 ctattgcaaa tatttacaga gtatttccta tatcatttag ctttatagtt acaggactat
     1921 atttcagtta taaaaataat aaaaataata agattaactt tgaggcagtt ttagaggaca
     1981 aaaaaaactt aattttctta ttgagcctta tgtttggaat gtacttgtat ctatgtttga
     2041 gtttgtcaat atactatact gttggaaatg tattgatttt aaaattatat ggaatcaagt
     2101 ctgccataat agttttggta ggtaactata ttgcagaaag atatagcttt agaattactc
     2161 aaataaaagg ttatgatatt aaaaatcaaa tagaatatgt agaaagtgta aagacagagt
     2221 atgagattaa taaattgaat gatattgtat atacagattt actgacaggt ttctacaata
     2281 gaccttttgt gaatcaaaaa ctatcagaat ggacatcaaa tcactacaaa tttaccttat
     2341 gttttactga tttaaatagc ttaaaacctg taaatgataa atttggacac caatttggtg
     2401 ataaatacat atccactact gctcaaataa taaaaaaact tataaatgta gaaaattcct
     2461 tactatttag atatggtgga gatgaatttt tattcctttt acagaataca agttgtgtag
     2521 aagcagaaaa gataatgatt aatataaatt atgaattatc aaaaataagt aataccgacg
     2581 agtatcctta tgctctttcc ataagctatg gattagttga gtgggatgga atatccgata
     2641 ttgaaacttt aatatcaaag gcagattctt tgatgtatga aaataagctt aaaaataaat
     2701 ctactttaaa ctagctagtt cagatattct acaaaatata tattataaga tagtactaag
     2761 aatgtcaaaa tatatagatt ttgcaaatat tgaatacagt ttactaatga atgcactgga
     2821 tgtaagtgtt agtaaacacc tattagatga acactttact gttgtttggg ctaatgaccg
     2881 ttattatgaa atgttcggct atacctagct aaaaagttaa aaatgaccac tgtagccgag
     2941 ggaatagaga ctagttttca aaaagatttc ttgcaagaaa tagactgtga tatgttacaa
     3001 ggatacgtgt tctctagacc tttaccaata aaagactttg aaaaacttat gtttcaaaac
     3061 tcctccaatt aacaatataa aattgcttat tataagtgat attattgtat caacaaataa
     3121 aggaattgtc tcaaaattga ttttttctaa tcaaattttt attttgagac attctcattt
     3181 ttttgcataa gaaaataccc accaagctac ggtttttctt accgctgttc ggtgggtatt
     3241 agtagtatta tgaaatctga aaactttaag tattactact tccgctagcg caatcacaaa
     3301 ggtcgaaagc ttcaaagcga aaataggctt tttactcatc aaacgtcacc gtacctgcca
     3361 acccgtccac cgttatcatt tgtccgctct taatagctcg tgtggcaatg tgtgtagcca
     3421 taacggctgg gatgccatat tcacgtgcga caatgcttga atggctaaga ggaccaccaa
     3481 tgtccgttac aatcgcactt gcagatgaaa ataatggtgt ccatgctgga gttgtggtaa
     3541 ctgcaaccaa aacacttcca ggttgaaagc tttcaaaatc tgctggacta ttcagtacac
     3601 aagctggagc cgtcacaaca ccagtactgg taccaatgcc cgtcaacaca attttcccat
     3661 ctttctgctt ctgttgtgct tgtgatatag tttttttgtt tttctctggc aacttggatg
     3721 gtgacacata acccatgtat tttttaagtt cagcttttcg aacaggtatt atcccgttca
     3781 tatcagatag agacttgttt ttatccaact gctctataag tacctccaat tccgattttg
     3841 tgagccaata aatatcatct aaatgtgaaa tagctcctcc tcgtatgaat cgttctgaga
     3901 tttcctgaaa catacaacga atcagaggat gtcccattcc catcaaataa atagcatttt
     3961 cacgcatagg agaggtttcc tgtgcccaat ggagcaactt aaaaaatagt tttttacgca
     4021 atccacctat gtgttgtaaa atctcttctt ctgcatgttt tctacgcttt tcaaacgtgg
     4081 tctggcgtaa aaaaggactt tccccttttc cttccacaaa tgtttttacg gattcaaaag
     4141 ttggtgtaag tgtttcctgc ggtgtggaat gcgcaaaatc aaactcataa gcggtacgac
     4201 caaactcttt aaagtactta ttgattcgat ttttccattc tatccatact tcctgtgaaa
     4261 ctccaacagg cacaactgaa gacatgaaat cttctgctat ctttattgca ggactgtttt
     4321 gcagataaag gttgatgctg ttattttgct ttacccattc tgaaataccc cacaactcct
     4381 tttccgattg cagaacaatc gtatcaaacc caagcaggaa aacggaagta tccgttatac
     4441 cagcacgtcg aactgctcct tgaaataatt ttgtaaataa tgtttcactg atagaagcag
     4501 caggcaatgt aagctgaatc cttgtgaaat aaatacatgc ggcataaaat attgtctgaa
     4561 taccctccat aatttgatgg gcgttcatag tatgcagggg cttttcttcc cattgtttaa
     4621 tagcatcctc atatcctttt cgtgccgttt cccagcgtgc aacgctgttc ataagtgtcc
     4681 tacgtaaaga gcgtggggaa agggatttga tagcaatcaa aagtggattt gatttagcag
     4741 aaagataaac atatccatta ataaccgtat atgccccgtt ttttggtaat aattttttcg
     4801 cacttttacc aaacatatct atccataaaa gctctgatgc acggtttacg atttcaagac
     4861 caagcgtggc gaacaaaggt gtaacaggat ttggcaaatg ttctgccaaa cttccctttg
     4921 tgtatacaac atcctgttct ggcaataccc attctggtgg cagaacagta attggacgag
     4981 cctgaacaat gtacaattta tctttttcca gtgcccactc tacatccata ggcatttgat
     5041 aatacttctc aattttcttt ccaagctgta tcagttgcat tacctgatta cgagtaagag
     5101 catatttttt tctcaacctt tcaggaacca tggtttcttc tgttccttct gaagtacgaa
     5161 ccgtcataat ctctttattt gccacttcat acgatataat tcgttcggag tccttatcca
     5221 ctacgattgt atcgggtgta actaagctag aaaccaccgc ttcaccaagc ccccatgcag
     5281 cgttaataat catttcgcta cgtctaccat tgattgggtt taacgtaaac ataataccag
     5341 atgaatcgga aaatgcaagc ttttgtacta caacagcaag tgcgacaatc tcgtgcttta
     5401 tattattctt cacacgataa gcaatagcac gagctgtcca taaggaagcc cagcatctct
     5461 ttacagagtc aataacttta tcttcacctt gtatgttaag ataagtttcc tgctgacctg
     5521 caaaagaagc gtcaggtaaa tcttcagcag tcgcagacga acggacagcc accgatatgt
     5581 tccctaatcc agcataagcc attttaattg cgtccgacac ttcctgtggc atttctccat
     5641 tatggaaaag ctcccctatt ttcttagata catcttcaag ttggctggta ttattggaat
     5701 caataccatc tagcagttta ttaattcgag actgaatatg gttcgtctca acaaaaatct
     5761 ggtatgaagc agtcgtaaca tgaaaaccgt ctggcacagg gatgcctgct gtcaatagtt
     5821 ttgataaaga catccctttt ccaccaacaa tttcaatggt tgcttgttta tgttccagtg
     5881 gcaagacata ataaatcata atcattcacc tccatcattc ataattccgt gcagaaatat
     5941 attcattgtt tcatccaaca ttttttccga actatccaat ggtcgtgacg tgtagctgat
     6001 tgataacatg gagttaatta acaaacgtgt cacaagagcg gtatccgttt tgcgaaagat
     6061 gcctgtggtc acaccattat caattaccga ttttataata tcttgatatg cataacgagc
     6121 atctttaagt ctctttcgat actcttcttc taaataatca gattctgtat ttaaccacat
     6181 cagtacagtt ctatactgct ctggaaatct aagatgatta agcatgattt ttctaaggca
     6241 ctgttgtggt gaaggttcac catcaataat cctatgaact tgttggatag ttttttctat
     6301 ttctttctca actgcatata caacaatctc gtctttcgtt ttgaaaaaat cgtatagact
     6361 tgactttccc atgttggcta aaactgcaat ttctctcatt gatgtttttt ggaatccatt
     6421 ttcagccata agactcaaag caacagcaac tattttttct cggcgttctg cttgttgttc
     6481 gggtgtaagc acaattcctt ttggcatagg gcgatacctc cttttagcga ccactggtcg
     6541 tttctttttt atttatcata cagcgaccaa cggtcacttg tcaaggttaa tttttttcat
     6601 tgtatatggt actcgagata aaaatgttca ttttagaaat tgctctattt aagtagaaat
     6661 aggcttcatc attagaaaac aagactataa atttgagata tttatcatgt tcaaatttca
     6721 tcaatatcaa agtttaagat attaataaaa aaagaattac taagaaataa catataaaga
     6781 ttcaaaaaag gagttgaact taaaattcaa ttacaaaaaa tcaaatttaa gacaactcct
     6841 ttattcttta tctctactat tctttatctc tatttattta ttacaactcc ctaacattta
     6901 ccactactgc cttaggtcta gtcatagcct cgattgaata cttaacacct tgagttccca
     6961 taccagatgc ctttacacct aaaaatggga agtggtctgg acctctttca gtcttattat
     7021 ttatttgaac agtaccaact tcaagtttat cagctatata aaatgcctta tcaatatcac
     7081 ttgtaaatac tgatgattga agcccatact ctgattgatt agcaatttca attgcttgat
     7141 ttatatcttt aactcttata attggaagta ctggaccaaa tggttcttcc caagcaattt
     7201 tcatatcaac atttacatta tctaacaatg ttggatagaa taaattattt tttctaacat
     7261 tgcctgttat taactttgct cctttatgta atgcatcatc aaccaaccct tgaacaaaat
     7321 cagttgcttt attatctata agaggagtga taactacttc atccattgga tttccaattg
     7381 tcaacttctc taccaatggc tttatttttc cgactaattt atccgccaca ctctcttgta
     7441 ctaaaattct ctttactgcc gtacatcttt gtcctgaata tgaatacgct ccagatacta
     7501 tattcttagc tgcaaaatct aagtctgcat cttctaatac tatagccgca tcttttccac
     7561 caagctccaa taataatggt accattccag atattttaga tatatgctga ccaacttcag
     7621 tactaccagt aaagttaata aaattaattc ctttatgcgt tacaacataa tctcctatct
     7681 cactacccct acctgtaata gtattaagta ctccatctgg tatcccagct tttctcataa
     7741 tttctaccac atgaagtgcg ctaatagccc cttgtgtagc aggttttaat acaactgcat
     7801 ttccaccaat aagtgctgga gcaatctttg acattgataa atttacagga taattaaaag
     7861 gagatatagc taaaacagta cctaatggaa cccttgaaac ataagacatc ttatttcttg
     7921 ttccaccagg gaaattatct ccactaatgg cttcaccctc catatttttt cccacatcag
     7981 ctgtgtatct taaaaaatct gctgttctct caacttctgc ccttgcagat tttatgtctt
     8041 tggctatttc aagtaccagt atattagcta tctcatctat attttctaac aataaatcag
     8101 cagccttatg aaatatattg gctctcttat atacaggcat ttcagcccat acttttatac
     8161 tctcttttgt gttttttata acctcatcta cctcatgttt tgtcatagat tgtactttcc
     8221 caacaagaga attatctatt ggtgagtaaa tttctattgg ctttctcgaa ttagattcta
     8281 cccattttcc atttattaaa tttttatata cattttcttt tgttttaagt tcattaaaca
     8341 tatttttatt cctctctctg atataacttt gtgtatttaa attatagcca tttattcgtt
     8401 atgtaaacat ttcttttttt cataaacgtt tacctataaa aaaattttaa gttaacgttt
     8461 ttctacattc ttctatttta ataacatact cgctccaaat ctatataggt cacactttaa
     8521 tatcaatata ctaatatacc aatataccaa tataccaata taccaatata ccaatataaa
     8581 tattataaac ataaatcact tgacaaattt ataagtagac aatattatat atctatgact
     8641 taaaatattt ttaaagtcat agatatcata tatagataga aaggattgat aaaaatggct
     8701 aaaagtttta cagaaataga aaaagacaat ataagaaaaa gactaatttc agaatgtgaa
     8761 atcaactggt ctaaatatgg atacaaaaaa actaatattg atgaactttg ttcaaaatca
     8821 ggaattgcaa agggttcatt ctacacattc tttgattcaa aagaaaagtt attttttgaa
     8881 gtattaaatg gaatccaaaa cagacttata tcatcagtag atgaaatttt aagtaacatg
     8941 cctccaaaag aaggctttgc aaaagcctta aagatgttgt atcgattata tgataaaaac
     9001 ccattcttat attcaccaaa taatactgat tacatcactt tattgaataa gatacctaaa
     9061 gagatgcttt cacaacttga atacagcaat gtacaaagtt tttatagctt aattgaaaga
     9121 cataatctca agttaaaaat agagaaagaa aaagcctttg gtgtttgcaa tgccctactg
     9181 tcaagcctat tgctaaaaga agatattgga tatgactaca ttgaagtatt tgattttatg
     9241 gtagacaatc ttgtagaaca catacttgag taataattta aaataaattt tctaaacatt
     9301 tctttataac taggaggagt aatatgattg aattaataca aagactaaat gatattattt
     9361 acaaaataaa tgataaacta aatatccaca caaaacttgt agaccctagc agtgagctca
     9421 tgccacttta ctctatgtta tctataatcg tttctatatt tctaataata ttaggtatta
     9481 aagtaagaaa aaaattcctt ataataattg ctattatatc aattattata aatattttaa
     9541 taatcttaga taggtaggta catatatggt aaaatcctcg aacactttta ataaaaactt
     9601 caacttgatg atattaggtc aaataatctc attgtttggt gcatcaatac ttaaatttgc
     9661 tttatcttta tacatacttg atataactgg taaagcagaa atatttgcta ctatactagc
     9721 agtatcttca atcccaatta tagtattctc ccctattggt ggagctattg cagatatatt
     9781 caacagaaga aacttaatgg ttatttttga tttttcaagt agtcttacag tactaatctt
     9841 agcagtattc ttattcaaca ataatggttc aatattactt gtaggaatta ttatgaccat
     9901 attatcagtc ataagcacaa tgtatcaacc aactgttcag tcaagtacac cccttttagt
     9961 tgataatgag catttaatga atgcaaatgg tattgtagct ggagttgcat ctcttacaaa
    10021 tattgcagga cctgtattag gaggtgtctt gtatggtgtg attggaatta atgcaattat
    10081 agttataagt tgtgtaagtt tctttctatc tgcaattatg gagatattta tacagattcc
    10141 ttttatcaaa caagagcaaa acagcaacat tataaaaact atatttactg atataaagga
    10201 tggtattgtt catatatgta aagaaaatag atttatacta aaaatacttt ttttagctat
    10261 agcaataaac ttatttttaa gctctatgtt aatagttggt atcccctata tagtaaaaat
    10321 cactatggga gctagtaact tcttatatgg tctagcagaa ggagcaattt cttttagttc
    10381 actggtagct gctgttagta ttggtatttt ctcaaaacat ctaaaaatca ctaatttgca
    10441 tatttccttt attatatgtg cagttatgtt cataccaatg gcggtttcag tatatccatt
    10501 tatattaaac acaggatttc cagttccatt tttaatcttt atagtatcat ctatgctaat
    10561 attttttaca acgactctta tctcaatata tgttataaca gtcacacaat taaaaactcc
    10621 aaatgaactc ttaggaaaag taatgtcaat tctttttgca ggaacagcaa ttgcaatgcc
    10681 ccttggtcag gttatgtatg gtaaactttt tgatttatta tcagatagag tgtacatcat
    10741 tatactagga gttggattta ttacattact tatagcattt gtatcaaaga taacattgag
    10801 gaacgaaaat ctttagatac aaagaattta gatataaaga tttaattttc actaatttct
    10861 gaatttaaat gcaaaattgt gtatcagtat gttgacgttt tagaaattga ttgatataat
    10921 caatggaaaa taaactttat aaaaattcaa aatattcagc tagaggagtg ataaaaatga
    10981 gttacatgcc aactttagaa caagcatggg aaatattaag aaaatacaat aaggaagaat
    11041 ttcatataag acatgctcaa attgtatctg gagttatgag atactatgca aaggagtacg
    11101 accctgaaag agtagaattt tgggaaatag ttggactttt acatgattta gattttgaaa
    11161 tgtatcctga tgaacattgt gttaaacaaa gagaattaat gacagagctt gatttagata
    11221 aatcaataat cgattctaca ataagtcatg gatatggatt aactggttca gatgttaaac
    11281 cagagcaatt tatggagaaa gtattatttg ctgtggatga acttactggt ttaattggtg
    11341 ctgctgcaat aatgcgtcct tcaaagagcg tttctgattt agaattaaaa tctgttaaga
    11401 agaaatttaa agataaatca tttgctgctg gctgttctcg agacgttata agaagtggtg
    11461 cagaaatgct tggatgggaa cttgataagc ttatccaaac aactatagaa gctatgagaa
    11521 gcttgattcc agagatggca atatagctcc tccatacata taccatagaa gttacatctt
    11581 taaagagcat ttctcttttt gatgcaactt ctttttatat tttgtagaat aaaaagttta
    11641 atattttaaa ctcactatat aatctataat aaatactata
//
LOCUS       DAMKMI010000121        11616 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMKMI010000121 DAMKMI010000000
VERSION     DAMKMI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.126"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            103..801
                     /locus_tag="PC429_002932"
     CDS             103..801
                     /locus_tag="PC429_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848420.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            979..1869
                     /locus_tag="PC429_002933"
     CDS             979..1869
                     /locus_tag="PC429_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848421.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(2262..3218)
                     /locus_tag="PC429_002934"
     CDS             complement(2262..3218)
                     /locus_tag="PC429_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF3848422.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3921..4916
                     /locus_tag="PC429_002935"
     CDS             3921..4916
                     /locus_tag="PC429_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3848423.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            4894..5757
                     /locus_tag="PC429_002936"
     CDS             4894..5757
                     /locus_tag="PC429_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF3848424.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFLKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            5754..6548
                     /locus_tag="PC429_002937"
     CDS             5754..6548
                     /locus_tag="PC429_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF3848425.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            6833..8212
                     /locus_tag="PC429_002938"
     CDS             6833..8212
                     /locus_tag="PC429_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3848426.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
     gene            8660..9373
                     /locus_tag="PC429_002939"
     CDS             8660..9373
                     /locus_tag="PC429_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848427.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(9645..11183)
                     /locus_tag="PC429_002940"
     CDS             complement(9645..11183)
                     /locus_tag="PC429_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3848428.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
BASE COUNT         4238 a         1329 c         1772 g         4277 t
ORIGIN      
        1 aactattaac taatatttgt aaatgtatac ttttgatgat aaaatataat taagttaagt
       61 tggtttttat aaacataaat ttattttaag gaggtgaaat aaatgaaaat atctagtcaa
      121 tatagaagtc aatattcatt tagatatgaa agtaatataa ataatacaag aataaatgaa
      181 agtatggtta agaaaaatga aactgtagga aaagacactt atttatctaa tattatgaaa
      241 caaaagcaag aacttaatga tagaattaga gatttaaaat atagacaaga ggtttatact
      301 aaaaaaataa atgacgcaat taagaactta tgtaaatcag aaataagaga aacaactaat
      361 aatttttcta atatagaaat aggtattaaa aatagcatta tagaagagaa aaataaaagt
      421 acaatgttag atgaaaattc aacttatcta aatacaaatg atgaaaaaga atctttaatc
      481 actaaagagt ctaatgaaaa aattgaagaa gaaatattaa atgatgaaaa attagaagag
      541 ttagaacaaa aaaaggatta taaagaggat tctaataaaa aagagaaagt atctgaggac
      601 ttatctttag taggtaaaac tcgtgaagag cttgaaaata tgcttaaaaa ttttataaat
      661 ttaacacaag aagaaataat gaaacttgag tcgagaatag aaaagttaga taaaaatgct
      721 gaagaataca aacaaaattc aaagactaat atatttgata aaacagatga acaaaaaaaa
      781 catataaatg tactgattta aacaatatgt tttaaaaaaa taagtttatt atataatgaa
      841 aatataaagg tacaatttca atacacttct attaaaccaa taatttatga atcagattta
      901 atttcaattt actaataacc atatatttat gagttaataa ttttatgttt taaaaagaat
      961 taaataaggg ggagtagtat gaagaagaat atattaacta ctatgattac catgattatt
     1021 atattgatga tgagtatgac agtaatggca gagccatatc aagaagatga aacatctagc
     1081 ttgaaaaaac agagtgcaaa tgaacgtaat cagactaata taggcacaat ggatggcaga
     1141 gtgcaaacaa atgcaataag taaaggtgct aattatgacc ctaaatatcc tcttaaagga
     1201 atgttaacac aacttggtct taatacaaca ggtaaagagg gatgggattt actctatgga
     1261 ggaaatccca atggagttga gcttcgagca gatggtaaat attattatat aaataagggt
     1321 atggaagggc taggatttta tgcggcagca ttgtcaggtg gctcatatca gagaataggc
     1381 tttggaaaac ttgaccctat agaagtaata gctgatggaa attggatttc ttacaaacaa
     1441 gctcaagata cacttacagt aatcaggaat tttttaaata gctttgactg gagaaatgca
     1501 tctgagatgg aaagagctaa tagagctgca aaattggtaa ccgaagcaaa atatgcagac
     1561 tctaaatatt gtaacatagt ctatggtaat ctggttgata aacgtggtgt atgtggaagt
     1621 tttgcaagta gctttcattt attaacaagg ttaatgggaa tggattcact atctatatta
     1681 aatccatcat taaatcatgc ttggaactat atacaaattg atgggaaatg gtataggttt
     1741 gatgggtctg aaatatcagc ttttggtgga tcattagact ttgattaccg taaattaaag
     1801 gatgcaacaa gagagatgac tacttactat gatgctaagg cattgtctat attaggattt
     1861 aatcaataaa aataggggga ggcttcttat agagcctttt tttattgatt tgaaatttaa
     1921 taaggaattt tatatgaaaa tttaatcaat aaaagcaaaa aaggctcttt ataaagcctt
     1981 tctaagtgtt tttttaagta aaatttttta tatcaacatc agttttaatc aaaacatata
     2041 acattttgga cttactttta aaaataaaat tattaaaata taatattaat catgttttta
     2101 gtttattata ttctataata gtggtcatgt tgactattgt agttttatta agaatagttg
     2161 gtttatataa atatatgcaa agatagcttg ttgtttactt tacttattag tatataggac
     2221 aagatactct aataaatttg cctattttaa tatcttttgt actaaaacca actaaagttt
     2281 cctttattat ttcgttttga ataatacaaa gcataatccg attgtttgta taaagactgg
     2341 aaagtatcag agttattttc taatttagat atacctatag aacaagaatt actctctagg
     2401 cataattcta aaaccttctt tgccttattt tcaacattct cttttgattg attactatat
     2461 tcaaaaacag caaattcatc tccaccaagt cttccaataa tagcatcatt tacgaatact
     2521 ttagtcaaaa tatttgaaaa atgtattagt atttcatctc ctcgaacatg accaaagata
     2581 tcatttacct gtttaaaatt atctaaatca aagatgtaaa agtatccatg tgaaacagtt
     2641 ttaagcattt cttttatgtg tacttcaaaa aaatgtctat tgtatagtcc agtcataagg
     2701 tctttttctg attttagttt aaattcattt gccattgcta gtgcttcttg tgctttttca
     2761 gttattgttt tagggtaata ctcatctttc atttggtcaa agtttggaat agaatggtga
     2821 acatggataa gtttaatcat atcatctgta actctgtata ccatagtaaa tcgagtatcc
     2881 atttctacat agatatcttt ttctaggtaa tctttttcta ttacccaaag agtaccatat
     2941 actataaaag cttcgtctgt aactttggta catgaatacc actcatctat aatctcaaag
     3001 tctatttgct ctgattctgt ttggtctttt tcaagaccta taataaattc ttctaatgtt
     3061 tcgtaaaatt catgttttcc tgttccaatt aatgcaaggt tgtcatctaa taaggaaata
     3121 ataaatttaa ggttttcttc ttctggattt aaaatataaa tatgccaaat tttttttgat
     3181 aaatctctta attgtttttg tattgtatta ttttccatag attaccccct taagatttac
     3241 agtttatatt ttaatgtatt ataagttgaa ctcttattac aatattatta taatatagaa
     3301 ctaaagaaat tacatcctgt ataagtaaaa gtatatcttt tataataaaa atattacagt
     3361 aaaataacca gttttaatat acgtattata tttttaattt ataaacgaat attataaatg
     3421 aaatttaaat tcaaccctga agaaataagc taaatgtatt agtatataaa tccatacttg
     3481 aacataaata acctttatga tatgatatca ataataatat tatgagagga gtgccttgat
     3541 gtcggagtta tataatggta accaacaatt aaatagttgg agacacatat attctgtata
     3601 atcagaaatt tcaagtgtat ttttaataca tttaattttt gtgtcttatt ctgcctttat
     3661 agataactga catatgacaa caaatagtat ttaaaatata tattactttg ttaaattatg
     3721 gtgtagtagg ctttaatctg ctacactttt attaattttg tatagtatta ttttgataac
     3781 tattttgaaa tagagagcat taatttttct agtccacagg agttttctgt ttttgtgctg
     3841 ttaaatataa gcacagagaa aggcagatat atacctgtgg acttttttta tataaattat
     3901 gaaatatgga ggtagttaaa atgagtataa ttgaacttaa tgaactgact aaatcttata
     3961 gaagatttga gaaaaaggca ggatttatgg ggagtgtaaa gtctttattt aataggaaat
     4021 atacagaaaa aacagctata tcgtcatttg atttaaagat agaagaagga gaatttgttg
     4081 gattaatagg tcaaaatgga gctggcaaaa ctactcttat taagatgtta actggtatta
     4141 ttgcaccgtc ttctggagaa atatctgtaa tgggttatta ccctaacaaa ttagaaaata
     4201 attttaaaaa acaatatgct gttgttatgg gccaaaaaag tcaactattc tttgaattga
     4261 caccagcaga tacgtttctt ttatttaaag aattatatga tatatctaat gaagaatata
     4321 agaaaaactt ggattacttt atcgaattat ttgaggtatc tgactattta aatgttcaag
     4381 ttaggacgct ttctttagga gaaagaatga aaatggaact aatagtagcc cttctacata
     4441 atccaaaaat attatttttg gatgaaccaa ctataggctt agatgctata gcccaaaaac
     4501 aaattaggta tttcctaaaa gaagtaaatg aaacaaaagg aactactatt atacttactt
     4561 ctcattacat ggaagatatt aagagtctct gtagtagaag tattgttgta gaaaatagcc
     4621 gtaaaatata tgatggttct acagagaaac tatttaatta ttttcaaaaa tataaaaaaa
     4681 taacagttag tttttccaat gaaactaaca ttgtatttcc aaaagattgt aaagttgttg
     4741 aacaaacaaa tcataagata tcagtaattg tgccaaaaga aagaggaaat gaaacgatta
     4801 aatggatttt tgataaatat gatgttaaag atgtaatgat tgaagaagaa gatataggta
     4861 gtattgtaga gaggatatat aagaaaggaa gtgatgagta ttatgagcaa ctataatgaa
     4921 gcaagttcaa atgattcaat gttttataaa tttaaacaga gtattagaag gtattatgaa
     4981 gtagccaaaa ttaactttaa agtgcaactt atctggagat ttgatgtggt ggttaatatg
     5041 gtgtttacga ttgtaaaaat tttatttgct tatattttat ggaaagcaat ttttggtgaa
     5101 aataaaatag tctctggttt tacatttaac tctatgttat cttactatat aataagctca
     5161 tttttagcag gtattgatat gtcaaagagt accagtgaag agatatgtaa tcgtgtaaaa
     5221 ttgggcacct tttcaaagta catgattatt ccaattaatg ttcaaaatta ttttattgct
     5281 aaaaattttg gaatgacatg tttttatttt gtatttaata tgatttctgc atttgtatgg
     5341 atttttatat ttaaaataga ttttgccttt acatcaaatc cagttcaaat attaatttca
     5401 attttgatga tttttctagg gtttattttt atgatgaaac tccacttctt tattgggatt
     5461 ttgtcattta aatttcttga gattagtttt ttaaaaatga taattgaaaa tattgttgaa
     5521 tttattacag gaacaatgat accacttgta ctcttaccag aaactatagt aacagttatg
     5581 aaggtatttc cattttatta tacaacatat ttgccatcta tgttactaat aggtagaaac
     5641 agcaatgagg caatacaagg attaattatt ctagcattat gggttttatt tttttctgta
     5701 gtaaataaag ttatgtataa aagaatgaga acattatttg atggggtggg tatatgatga
     5761 aaaaaaattt gaaaataata tttatgttat taaaaatgaa attatcaaag atgatggtat
     5821 ttagattcga tttttttgga gcattttttg tagatagttc attatttata ttacaacttc
     5881 taatgtttaa tagtatatat tctcatgtag actcaatagg gggatggcaa caaggagaga
     5941 tgttaatatt tataggaact ttctcattaa taaatgcgat aaatatgacg attttctttt
     6001 ttggtattta tgatattcca cgaaaaatcc aagaaggaga attagactac tatattacaa
     6061 aaccattaga cccactattt aggttaacat ttgaaaatat taatattggt tcatcaccat
     6121 taattctagc aagtatactt attgttttat atggaatatc tgagttaaac tcagagataa
     6181 ctttagttat gataataata tatattttca tggtgttatt gatgacatta cttttttatg
     6241 atgtatgtat catacttaga acagtttcat tttttgttat atcatcttct gcgataatga
     6301 gattagaaga taatttattg ccaatgaata tgaaaattcc tggtgtaata tatgaaggta
     6361 tttttaaagt attattctat ttaatcttac catatggaat tatgtcgaca attccgactc
     6421 aaattttggc tgggacaata acaattaatg gattaatata ctcaatttta attgtatttt
     6481 tgtttacatt gtttatgtgg agattttgga agttaggtat gagacattat aaaagtgcaa
     6541 gtagttaata aattagtaaa aatagacaat gtataactat atataaatac gtttcatatg
     6601 acactaaact atgtgaacaa gtatttgcgc taaaggcact gaaagctata aaatgattaa
     6661 agtaaggatt taaaagtttc tttagtagaa atataattgt gtaaaaaagc aagattaatt
     6721 aaaactatat ccaaataatt agatataatt cttatgcaga aaggaggtct gtaaatgtct
     6781 aattgtttgg aatatatttt aaaaataatt aatgtacaga aaaataaaca aaatgagtga
     6841 tgtatattat tcagaaaatc atgaacaaga taatctttat gagcaagata atttactggc
     6901 acaaagattt aatttattta ctctattgaa gtttgcattt cctactattt ttatgatgat
     6961 ttttatgggg ttgtatacaa tcgtagatac tatatttgta tcaagattta ttaatacaaa
     7021 tgctctatca gcaattaata ttgtatgtcc aattattaat cttatagttg gaattggaac
     7081 tatgcttgcc actggaggaa gtgctattgt agcacgtaag atgggaaatg gtgatattga
     7141 gggagcaaat aaagcttttt ctttaatcgt attatcagga gttgtaattg gggtaataat
     7201 tactggtata ggtattgtat ttatggatta tatcattata tggttaggag caagtgaagt
     7261 tttaattaaa tattgtcgtt catatctttt tattatcttg gtttttgctt ctgcaaatat
     7321 actacaagta ttatttcaat gtttatttgt aacagctgga aagcctcaat ttggattatg
     7381 gctagtgtta ggtgctgggt gtgctaatat aattcttgac tatgtgttca ttgttctgtt
     7441 acaaatgggt attagtggag cagcacttgc aactggagta ggatacctaa ttcctaccat
     7501 ttcaggtatg gttttctttt caaaaaataa agggttactt aaattttgta tacctagact
     7561 agataaaaga atattatttg aagcttgttt taatggttca tctgaaatgg tgggtcaact
     7621 atcaacagca gttactactt ttttatttaa tgctatgatg atgaaattaa ttggtgaaga
     7681 tggggttgca gctattacaa taattattta ttctcaattt cttttaactt cgctttatat
     7741 aggtttctca atgggtgttg caccaataat aagttataac tatggaagtg aaaataatac
     7801 gcagttaaat catgttttta agatttgtat attatttatt tcagtagcat ctttattggt
     7861 atttatcttt tctatatcat gttcaccaat cattgtagag attttttctc aaagaggaag
     7921 ccgagtttat gaaattgcaa ggtccggatt ttatatattt tcttttagtt ttattttttg
     7981 tggatataat atttttacct catctatgtt tacagcatta tcaaatggaa aggtgtctgc
     8041 aatattatct tttctacgta catttggatt tataactata ggtttattga tattgccaaa
     8101 tatattaaat ataaatggga tatggttagc agtaccaagt gctgagattt taaccttatt
     8161 tttaactgtt ttttgtatat ttaagtataa gaatagatat aaatatatgt agatattaaa
     8221 taaatagctt agatagatat tttatgtttt aataagtaat tcaattagtg gtagatatat
     8281 actattgaaa attaaagtta taattgatat aaagaattaa taaaaataac atataaaagt
     8341 ttaaaaggga gttgaactta aagtttgatt agaaaaagtc aattttaaga caactccttt
     8401 tattttacta ttgtattaat ctgtatctgt aagtgtatta gtctaatatt ataaactttg
     8461 gatgaaatta attttctata ggttttaatg tatcatacac tttattttaa agaaacttat
     8521 cttaattttc atagtgattg aatttgtcta ttagagatgt tactattagt aatgtaaaag
     8581 caagagtagt gaatataaaa aacaagatag tgatactgca agattagaat ataataaatt
     8641 tttatggaga gatttcaata tggatacatg ttttaataaa aaaatattac ttgttgatga
     8701 tgaaaaggat atagtagatt taattgaaga agtactcata aatgatggtt ttaaaaacat
     8761 tattaaagct tataatggtc tagatgctat ttcgctgtgt aagatagcat gtccagatgt
     8821 tgtcattctt gatataatgt taccagatat agatggtata gaagtgtgta aaaaaattcg
     8881 agagttttca tactgttcta ttcttttttt atcatcaaaa aatgatgata ttgacaaaat
     8941 actaggtctt agtagtgggg gagatgacta tataacaaag ccattcagtc caagagaaat
     9001 tgcttttcga gttaaagcgc aattacgtag acaacagtat caaagcattg taccatcaga
     9061 tagtgaagtt attaaaatag gagatattac tattgatata gaaggaaacc gtgtatataa
     9121 agatagaaat gagattgagt taactggaag ggagtatcat cttttaagct acatggctca
     9181 aaatgtaaat aaaattatag gaaaagaaag actctatgaa caagtatggg gagtatatag
     9241 tagtatttgt gacaatacaa ttatggtgca tatacgacat ataagagaaa aaattgaaga
     9301 taacccctca aatcctaaaa tacttataac agttaaagga ctaggatata aacttgtgaa
     9361 tagaatcgat tagaatagat tttaatatta atgaagatat ctcaagttga atttttagtt
     9421 aatgaggcat tcttttatgg gtgaaatcaa atgtattttc gttatctcaa caatgaagaa
     9481 gaggtctatc tctattttga gatagcctca ttaagtttat atcctggtaa tgtactttat
     9541 gttctaataa aattttttta gtttatttgc agtgaaacat taactagaac attaaaattt
     9601 gcattgaaat accaattaaa taatattgta tattgtgtat aaacttatac taattgtttt
     9661 tctatattaa atactttatc aatccaacct ttataaaatt tttcttcatg ggaaactagg
     9721 attatacttc ctctaaactg tttcaaagca ttttgtaaag catcctttgt ctctgcatca
     9781 aagtgattgg ttggttcatc cagaataaga aaattacaag gagataacat catacaacat
     9841 aatttaacct tagattgttc accaccactt aaagtagata cactccttga aacatgctct
     9901 tccttcacac cacatctggc tagatgacgt ctaatctctt ttgtatttaa ttttggatat
     9961 ttatctgcta ctatttgaag tggggtttta tcaggatttt cccattttaa atcttgctca
    10021 taataaccaa ttttaacttg ttctgaaaac tggaaatctc cagatatacg aggaatatct
    10081 ttgactaatg tctttaacaa ggtagattta ccgattccat taaaaccagt tataactatt
    10141 ttttgacctc catcaacaga gaaatttaat tttggaagta aagaatagta gtatccaact
    10201 tcaagatttg ttatagtaag agcattttga gccgatattt caatttctga aaattgaata
    10261 tttggttttc ctgtaaaact aggaggagct attctttcta ttttatccaa ttgtttttgt
    10321 cttcctcttg caatttttga atttactcca gctttatttt tacgtataaa tgtttcttct
    10381 ttttctattt tcttttgttg tgcttgatac cttctaatat aatcttctct taaatgtgat
    10441 ttttgtttaa ggaactctga atattttcca tgatatttct taatcatgcc aaattctata
    10501 tcaagaatac ctgtagaaat tttttctaaa aagtcaaaat cgtgggaaac tacaataaat
    10561 gccccattaa atgtatttaa ataatttgag agccattcaa catgctcttt atctaaaaag
    10621 tttgttggtt cgtctaataa tagaacatca tggttagaaa gtagtaattt tgctaaaatt
    10681 acctttgccc tttgaccacc actaagctct ctaacaacac gattcattcc tattgaatct
    10741 aatccaagac catttgctat tttatttatt tcattatcta tagaatagaa attacttgct
    10801 attagttgtt cctgataatc tgatgctttg attagatatt gctcattttc agatatagca
    10861 cttttttgat agagtaaatt catttccttt tctattttat aaagttcttc aaatgcagtg
    10921 tgtaaataga gtgatattgt tgtatctcta ttgatttctg catattggtc taaatggcct
    10981 atattgatat taggttgcca cttgatttct cctgaatctg gaacaacttc tcccaataaa
    11041 attttaatta atgtactttt acctgtacca ttttggccaa ctacacctat atgttcacct
    11101 ttgaataaat caaagttagc tttctcatac agtgatttat ccataaaaca ttgtgataaa
    11161 ttagttactt ttaataaact cataattcaa actccttttc ttttattaaa aaatttatgg
    11221 aaataaaaaa agcagaggaa tggcattgta aaatgccgac ctctgctttt ggaaaaacaa
    11281 aaggtcatag acaagcattg ttgtctatga ccagaaaatc tatattatct atcggcaaat
    11341 aggtaatttt aaataagcat agccaaaaaa ctatactaaa cctatatttg ccgatgaaga
    11401 tagaaagctt taagctttca gattaacctc tacacaacgc ttcatcgtat acatttgtgt
    11461 agagttaagt tttctaacaa gtttaactaa ttctatataa atcataatta ttcacatcct
    11521 aatcttagta ttaattctag tttatctgta tttaaattgt acttttgtac tttgtggatt
    11581 atcttattta tttaaaatat gttttattaa ttatag
//
LOCUS       DAMKMI010000122        11614 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMKMI010000122 DAMKMI010000000
VERSION     DAMKMI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.127"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            64..702
                     /gene="recX"
                     /locus_tag="PC429_002941"
     CDS             64..702
                     /gene="recX"
                     /locus_tag="PC429_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF3848429.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            855..2858
                     /locus_tag="PC429_002942"
     CDS             855..2858
                     /locus_tag="PC429_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF3848430.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYENMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITYPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(2985..3296)
                     /locus_tag="PC429_002943"
     CDS             complement(2985..3296)
                     /locus_tag="PC429_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF3848431.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(3374..4192)
                     /locus_tag="PC429_002944"
     CDS             complement(3374..4192)
                     /locus_tag="PC429_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3848432.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            complement(4292..4816)
                     /locus_tag="PC429_002945"
     CDS             complement(4292..4816)
                     /locus_tag="PC429_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF3848433.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            complement(4835..5977)
                     /locus_tag="PC429_002946"
     CDS             complement(4835..5977)
                     /locus_tag="PC429_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF3848434.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            complement(6257..8644)
                     /locus_tag="PC429_002947"
     CDS             complement(6257..8644)
                     /locus_tag="PC429_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3848435.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            complement(8637..9320)
                     /locus_tag="PC429_002948"
     CDS             complement(8637..9320)
                     /locus_tag="PC429_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848436.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            complement(9437..10747)
                     /locus_tag="PC429_002949"
     CDS             complement(9437..10747)
                     /locus_tag="PC429_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3848437.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            complement(10741..11448)
                     /locus_tag="PC429_002950"
     CDS             complement(10741..11448)
                     /locus_tag="PC429_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848438.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
BASE COUNT         3961 a         1860 c         1282 g         4511 t
ORIGIN      
        1 gttttttgtt aaattttatt agttgctttt gttaatataa ttacatataa aaaaggagga
       61 cttatgggta taattacaaa aatagaacaa caaaaaagaa atgatgatag agttaatatt
      121 tatgtagatg ataaattttt tattgctata tttaaagaac ttgtttatac atttaattta
      181 aaaaagggga atgaaataaa tgaagaagaa ttaaaaccta ttttagatga tgaaatgtat
      241 ataaaagcta aaaataaagc attaaatatt ttatctcatg ctgaccaatc tgaaaaaaaa
      301 ttaaaagaaa aactatcatc agaatttgat gaaaatataa ttgagagggt catagatttt
      361 cttaaaagtt ataatctcgt aaatgacagt gtattagctc aaaaaattgt taacacaaat
      421 gttaacctaa ataaatgtgg caaaaacaga attaagcaga atttatataa taaaggtatc
      481 aatagaagta caatcaatga agcagtatct gaattagaca aggacactga atttgaaaat
      541 gctatgtatc tagcaaaaaa aaggtatgaa agagtaaaga aagaagataa aaagaaaata
      601 tatcaaaaaa tttctcaaca tctttcctat aaaggctttg attatgatat tattaaaaga
      661 gttttaaaca aacttttaaa ttttgatgaa tacgatatat aagtataaat actagggagg
      721 taaattatga aattaatcat tggtggctta agctttttat taggattata taatatatat
      781 caattatata gagaagatag tatgagataa atattatttt ttagatgtaa tatttattat
      841 ggaggagaaa tacaatgaaa agtcaaaaaa gatttaaaat tttagatatt gatgtatacc
      901 tacaaccatt tgaatctgat ataaaaaagc gaatgaaaca ttatgagaat atgaaatcaa
      961 aaacattgaa aaactataaa gattttagtt cttttgcaaa tgctcatctt tactatggat
     1021 ttcatcagac aaatgatgga ttcttctata gggaatgggc tccaaatgca gatagtttgt
     1081 cactaattgg tgactttaat aattggaata gaaaatctca tcctctaaga aaaattgggt
     1141 ctggtaattg ggaaatattt attcctggtc aaaatagctt acctcacaaa tcagaggtaa
     1201 aggtacaagt tactgcaaat ggaaaaacat ttgaccgtat accattatat ataaaaagag
     1261 taattcaaaa agattttggt ggatttaatg gacaaatatg gcaaccaaaa actccattta
     1321 tctggactga caatgatttt gacttaaaaa acataactta tccactaatc tatgaatgtc
     1381 atataggtat gtctactgaa agcgaatcta ttggaactta taatgaattt actgaaaaaa
     1441 tacttcctaa gataaaaaaa gctggataca ataccattca gcttatggct attatggaac
     1501 atccatatta tgcatctttt ggataccaag tttcaaactt ttatgcaatc tcatctaggt
     1561 ttggtactcc tgaagactta aaaaacctca taaacactgc tcattcaatg gggattgctg
     1621 ttttattgga tttagttcat tctcatgctg taaagaatac tttagaagga ataaatgagt
     1681 ttgatggaag tgaacatcaa tttttccact caggttcaaa gggaaatcat cctgcttggg
     1741 gtacaaaact ttttaactat ggtaaacctg aagtaataca ttttttgctt tcaaatataa
     1801 aattttggtt aaatgagtac cattttgatg gctttaggtt tgatggtgtg acttcaatgt
     1861 tatatcataa ccatggtctt ggagtttcat ttgatagtta tgaaaaatat tttagtatga
     1921 atacagatat tgaagctata acatatctcc aatttgcaaa tgaattaata aaagaaatta
     1981 aacctaattc gataagtatt gcagaagata tgagtggaat gcctggtatg tgtatcccta
     2041 taaaagacgg cggaattgga tttgactata gactcgcaat gggagtacct gatttttgga
     2101 taaaaactat ctctaactta tctgatgaag actgggattt gggaaaaatg tggtatgaac
     2161 ttactactag acgacctgga gaaaaaaata taggatattg tgaatcccat gaccaagcat
     2221 tagttggcga taaaacaatt attttttggc tagctgacaa ggaaatgtac tggaatatgg
     2281 aaataaactc aaataaccat gttatcaata gagcaatatc tcttattaaa ttaattaaat
     2341 taataacttt ttctctagca ggtgaaggat atctaaattt tatgggaaat gaatttggac
     2401 atcctgagtg gattgatttt ccacgagaag gcaacaattg gagctataag tatgccagaa
     2461 gacaatggag cctttcagaa gataacaact taaagtataa acaattatta aattttgata
     2521 aatctatgct cgaacttaca aaatacagtg atatttttat ccaaaatagt catgaactta
     2581 tacatatgga taatgataaa aagattttgg tgtacagtaa gggcaaatat ttatttatat
     2641 ttaatttcca ccctaatcta atacaatcag ttgatatttc aaagtttcaa aatacaaact
     2701 acaaaacgat attaaatact gatatggtag agtttggagg aaacacaaaa aaagaaaact
     2761 taagagatta tgatgtatac tttagctcaa ctcctccttc ttctaaaatg cctattgcct
     2821 ctcgtacagc aatagtgctt ttagatagta gtatttaagg ttggtttttc tagtatataa
     2881 cactcatatt aaaataaaca ctaaaagcca aataacaatt taaatacaaa atgttatttg
     2941 gctaatttag tttgatacta tttaactatc ttttacatta agttctataa tttccaactt
     3001 ttaatttgtg gtattatatt catcttaaca gtttgattct ttaaagcttt tctaaatttc
     3061 tcagttgaag aatctttaaa cattttacta gaagcactct tgaggtcttc tatagattcc
     3121 caatgttgta tcataatcca cttttgagac ttttcatcat ataaaagctc agtatcaata
     3181 aaaccagatt gaatagaatg aaattttaat tctaagtcat ttacaatttc tataaactct
     3241 tcttttgata aattttcttc cacattaaac tcaactattt ctgtaataat tttcatatgt
     3301 aaaacctcca atttattttc ctatatcata tcaaaaaata aattggaggt attgtaaaaa
     3361 atcgacagta taattataaa tagctgtact ttatattttc tttaatcgtc ttattctcac
     3421 caataaattt aattggtgac gtccctgaaa attttttaaa ttcttttata aagtgtgctt
     3481 ggtcatagta ctcattttca taaataaaat caaaaagttc atcatcttta taattataaa
     3541 taagttgatt gcttgccatt ttaaatctgt aaattctctt taaaatctta ggtgtatacc
     3601 ctgtatattt taaacagatt ctttccaatg ttttaatatt tatatttgta tcatcacaaa
     3661 atgcttttat attgctatat ctattaacat atataaatga atttagaagc tttacatatt
     3721 tatttgaaat taaatcttct tctaatatgg ataataatac ttcttctaaa tataaaacaa
     3781 ttttttctac tgattgtgtt ggatttaatg actcttccaa tttttttgca aatagctgtg
     3841 atactgcttc taggtcaaca atttgtctgt taaactctga aattggtatc tttaaaaatg
     3901 gatagagacc aaatggataa aaagaaatcc ctataacacg tatatttcca tgttgaataa
     3961 taaatccatg tttatccctt attccattaa aatgcatatt actagcagta tagtcttgat
     4021 tttcaatttt atattccata acatcagaaa gattgagaat aatatctata ctgtctgtcg
     4081 gaagtaactt attatttaac agtatatttg attttgtctc aaaataccaa ataaacttta
     4141 cccacggctt taaacgaaca tttttgattg aatatcttgt aactgataac attgtgaact
     4201 aacctctata ctaaaatttt ttaattatgt actccatcct aatattatac agtataattt
     4261 atgctatcat aaattttagt tcactttata tttattttat acattttttc aataccttag
     4321 aaataggaag cgctattata agacctatca atacttgcat taaattacct ggtattgaag
     4381 taagaggtac tataaaatta ccataaagta taacttctgt tacataatat ccaaatagca
     4441 tccaaattga tgaaacacat attcctatta cattgattaa aaaattattc ccattattac
     4501 catttgccca tgcaatcttt ccaagtaaat aacccataac tcctctaact ataaaggtaa
     4561 acggtgccca taaagtccac ccagatatca aatcaaaaag tgacatacca atagcccctg
     4621 ataaagcacc ttttttacct ccaaatacta ttgctgatat gaacaacatt gcagttccta
     4681 aatgcacaag cccaccattt atagatattg gtaatcttat gtttataaat ttagttgcaa
     4741 taaatactaa tgctattaat agtgaggttt ccaccatgtc tttcgttgta aatttttgct
     4801 tagctgtaac ttccatacta tatcccccta tctattaaaa ataatactta ttaaataaat
     4861 ttaacaaatt tccatttcct ttgaatccta atgcacttaa acttttatcc aaaacattta
     4921 gtacatgttc tattttattt agatttgcat tctctcccat atgacctatt cttaaaatag
     4981 tatccatata ttgatttagt gatgttgcaa caagtgtatt ataattttta agcatataat
     5041 cagtaagttc taaagctcct atactttcag gaatttttat agctgtgact gtattagaat
     5101 atccttcttc taaaaatagt tctagaccat attcctcaat agattttcta acactataag
     5161 ctatcttttc atgtctattt aatacatttt ctattccttc ttctaatata ttatcaagtg
     5221 ctttatcaag ccccattatg tcacttattg gcattgtata agggaagtat tttttctcat
     5281 aatagccttc ccatatattt aaattacaat aaaaacctat tacatcagtt tttctatttt
     5341 ttatacaatt ttttgcatct tgacttattc cgactatagt aagtcctgct ggtgcagaaa
     5401 tagctttttg tgaaccacct aatattatat ctattttaga ttcatctaca cttaatcttt
     5461 ctcctaccat acctgctaca gaatcaacta cagttaatat tccatatttt ttaagtaatg
     5521 gacatatttt gcttacatca tttaatacgc cagttggagt atcacaatgc actactgttg
     5581 catatttaaa attactatcc tcatctaaaa atgctttcaa ttcatcaata tctatacttt
     5641 tagtatactc acttgaaaaa agcacatact ccccaccata cattgttaca aaatctttaa
     5701 aaccttctcc atatatacca ttgtcaatta caagtactct atctcctttt tctgtaagtg
     5761 atgcacatgc tgcttcaaga cctaatattc cttcaccact caatatgtaa acatcgtttt
     5821 ttgttccaat tatattacca aacttctcgc atgtcttttt ataaaattca acaaaatcaa
     5881 tatctatatc tggatttgtt gttttttctg ctcttgctaa tcttacattt tccctagttt
     5941 cagttggtcc tggtgcataa ttaatcttct tattcatata tatcagctcc tatttatttt
     6001 ctatacgatt attatacaaa attttcattt tatatggtac aattttatac ttttttagaa
     6061 ttgtaccggt acattttcta acatgaataa tatcagtata ttagatatat tattgattac
     6121 ataataaaat acagagcaaa catctgttta aattgtttgc tctgtactat gctttaacct
     6181 atttaattat gaatctcttt aactatattt ttttatatgt tttttgctag ttaaactaac
     6241 cataaatatt tgcattttat tcactgtatc ttactctatc aataagagat tctttattaa
     6301 aacttcgtcc tatcaaataa gttgttataa attgagctat aagaatacac acaatcatca
     6361 acaagattgg tactattggc aaagaatatg ttgcataaga taatccagtc ttttttagaa
     6421 atataacagc aatataccct aatatacctc caaatagaat acttcctatc atcattccag
     6481 aagtgtaata tattgcctct gaattaagca ttttcactaa ttgtttatta gtaagaccaa
     6541 tagcttgaag cataccaagt tctttttttc tagtaactat gctagttatc attgtgttga
     6601 caagattcat aaatccaatc acacctatta tgcctgttag cgtgtatccc attatcttta
     6661 taaatgaaat agtagcctta tttgactcaa ttctactatc tataaaatca gtctctaaaa
     6721 atccattatt tttagcaatc ttcttgatac tatctttgat ttcttgatac tttccttctt
     6781 ctgtatatat tcccaagcta ctagttgaat cgtatttcaa agttctatct ataaaatctt
     6841 tccctatacc aaaacttgga acccccatag ctattgcttg aactttaaat tcttttttta
     6901 tctttttatc tccatcatat aaagttaatc ttattttatc tcctgctttt actcccattt
     6961 cttttgctgt atcaacatga gtaattacta tttcatctcc agtttgaagt ctttcaagat
     7021 ttatttttcc atctattata ttcttgttta atgattttac atcattttca tttatacttt
     7081 gtaaaaggtc atacttaaat tcaacatcat agtcctccat accaattttt acacttcttg
     7141 cagattctat tcttttaatt ccttccatgt tctttatttg attaaaaaaa tctttactta
     7201 gaggattatc catttgtaaa atattcaaat tatctttagg atttttatca ctgttcattt
     7261 cataaccaga tagtctaact tctatatcat aaggaaagtg ttctcttgcc attttctcag
     7321 catcaaaact ttctaaggca gttgatactg ttataaatat agttccacta agtataagtg
     7381 atgccaatgt catgtaagta cgttttttat ttctttctaa attggcatgt gataatctct
     7441 ttagatttat atttttatat ccttttctct tactcttatt acttatttta tttcctgtat
     7501 atcttactgc gtcaacaatg gatacttttg atgctacttt cattggtttt aaaagtgata
     7561 aaacaacaga tataaagctt attaccacaa ccgataatat aactggtaat cgtgatgatt
     7621 ttacatctat atccataaat gattttatta taatatttgc aagtacatag cctaaaatta
     7681 ttcctattgg tatagctatt ccggctaata tgaacccttc tttaaacact atatttttta
     7741 tttgtttctt agttgcacca attgctctaa gttttccaaa ttcttgaacc tttgtaacta
     7801 ttgaaatata aaatatattg tatattacta aaatactaga taatacaatt actaacccta
     7861 caaaaactcc acccataatc gtttccatat ctggttttaa agcatttata taattttcat
     7921 ttacagcagt attaatagta tccagtccta tatcattagc aattttatct aatttttctt
     7981 ttgcttgctc tattgataaa ttacttttat cattaaattt cacataaata ttaaaatctt
     8041 cttgtgacat atctctagta tttctcatat atgattctga tataatagca taataatatt
     8101 tacctgcttc agatatttcg ctagttttta agattccact tataatgaac tctttttcaa
     8161 tttttttatc atttttatag tcatcataag atatttttat cttttcacct aaatctttat
     8221 ttttataacc aagtgccttt aaagctccac tatctaacac aatttcattc tcttttgatg
     8281 gcaattttcc tttgataaat tcaaaactat ttaatttcaa agcatcttca ttcatgtatg
     8341 ctagagaaat atttgaatca tcttccagtt tttcaacacc tactccattc atttcaccag
     8401 ttaattcaac atctgcatga ttactcaata tatcagcctg tttttcatct acatttgcta
     8461 atataccttg ataattgcct gaatacttta tagtattttc tttttgatat gctcctgcat
     8521 caacaatata aattccaata gaagaaagca ttaaggttga aagtattata gtaattatta
     8581 ttagaatact ttttgattta ttttgcttta aattactttt tgctatttta gtagtattaa
     8641 tcatttttat caccaacctt tgacaccctg ccatcttcta tgaatataac tctatcagcc
     8701 atttgggcta tactgtcatc atgagttatc ataactagag tttgactgta cttcttagac
     8761 atagatttta atatactcat aacctcatca gacgttttag aatctaggtt tccagttggt
     8821 tcatcagcaa gtattatagc tggtcttgta gctaatgctc ttgctattgc caccctttgt
     8881 tgttgaccac ctgataatgt atttggtaaa acgtcgtgtt tattttcaag tcctaatgat
     8941 tttaaaagtt cttttataaa agattcatct acttctctac catctaaacc gattggcaac
     9001 actacatttt cccatacatt aagagatgga attaaattat agctttgaaa tataaaccct
     9061 atttttcttc ttctaaatac agcaagttct tcttctttta atgtaaatat atttttatta
     9121 tctatataca ccttcccaga tgtaggtata tcaagacctc caatcatatg caaaagtgta
     9181 ctttttccag aacctgattt tcctattatt gcaacaaatt caccttcatt tatttctaag
     9241 tcaacattgt ccaaagctct tactaagctt tctccttttc cataatgctt tgataaattt
     9301 tcagtatata gtattttcat aaaattatcc tctcattcta agttatatta ctccatattt
     9361 tattaatttt ttatttctgg agctgtttat acttatataa taaatgtaga ggcttacatt
     9421 ttcctttcat ttaatattac atagttgtaa gaaacaaaga aaacttgctc cctattccta
     9481 cttttgaaga aactataata tttccacctt gttcttctaa aatctttcta cttaaataca
     9541 atcctacccc actaccttct acagattcaa tttcatcagc agaacctcta taaaaacgtt
     9601 taaatatatt attaaactca tttttttcaa tccctatgcc attatcctct attataattt
     9661 taaagaaatt agcagtttct tctacagata tatttatttc tccatttacc tgtgtatact
     9721 ttacagcatt gtctaataca ttaaaaatac tctcctcagt ccacttttta tcatgtggta
     9781 taattctact gttaaattca tttaaattta atgttatact tttgctctta gccttagggg
     9841 taacactttt tactgcattt agaatagtat tttttatatc attatcttct ttttttatgt
     9901 ttatcatgct aatttctaac cttgatatgt tcactaaaga atttattaaa ttttctaatt
     9961 tatttataga agccttattg gttaataaga actcgttggc ttcatcttct tctaattctt
    10021 cttctataag taatgtattg tataatttta aggatgctaa aggagttttt aattgatggg
    10081 aaatatctgt tactaatgat tttacacttt ctttttcttt ttctaattta ataaacttta
    10141 tctttaaact tctattaagc tcatatagac agttatttat cttactaaat atgccttctt
    10201 taaagtattc attatattct actagataac cagtttcttc tgactcagta gtgtccttca
    10261 agtagttttc ttgtgacatc ttatctacta taaaataaat tttactcaat cttttattta
    10321 tgtatcttat aaaataatat attataccta taactactat aaacatcatt aaaaaaataa
    10381 aaaatgattc cttaaaaaaa gattttaagt ataagctaaa atttttatca tttgacattt
    10441 tattttccaa tccatagcca aatttcttaa atgccttttc tccagtagac accttactat
    10501 aatcagaatt ataaatagaa tttactatat ctatttcatc attaggatat ttttcaatca
    10561 atattccagc aatattttga gtgtttttta tttgatgttc atacaattta tgtaatctgc
    10621 tatattcaaa aagtataaat acaagggtta ctattagtac agataaaaca gaatatacaa
    10681 taactcttct cattatataa tcatctttca cacttaaacc atctttgagg ttcatatttt
    10741 ttagcatttt tttatactcc tttctcccca catatatcca acccctctta ctgtttttat
    10801 atatttaggt tcagatggat ttttttcaac cttttgtcta agtcttctta tatttactgc
    10861 aatcgtatta tcatctacaa aattaccact actatcccat aaagattcta acagttgctc
    10921 tttagtcaat gtctgcatag aattttccat caaatatttt aatagttttg tctctgtctt
    10981 acttaaaatt atctcttctt ccttatcatc tgttcttata attaatttat tttctatata
    11041 ataaaagaat aaatcatcac aaactaatag agtataattt tttactgtat ttactctttt
    11101 cataagcgca ttcacttttg atattaatac catcaagcta aaaggctttg tgatataatc
    11161 atctgcacct aaatcatatc ctgtgacaat atccacctct tcatcaagag ctgtcaacat
    11221 tattatgtaa acatcacttt tctttctaat ctcctcacaa aagtcaaaac cactaccatc
    11281 aggtagacct atatcggaaa taattaagca tatctcttct tttgcgaaaa tattttttgc
    11341 ttcttctata ccaaatgcac taaaaacatt gtatccttct ttttttaatt taaaactaat
    11401 tcctctattt aaagatttgt catcttccaa aagtaatata ttattcatcc ctatatcctc
    11461 catcatcata tatactagct aaattatatc atactaataa aatcattttt ttatcaattt
    11521 gtaaatatat cagaagttgt taatatgatt tcatttactt gaataaaaac taaaaatccc
    11581 cataatacta tgccatttag tgtaattatt tttc
//
LOCUS       DAMKMI010000123        11568 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMKMI010000123 DAMKMI010000000
VERSION     DAMKMI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11568
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.128"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(318..980)
                     /locus_tag="PC429_002951"
                     /pseudo
     CDS             complement(318..980)
                     /locus_tag="PC429_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(1140..1676)
                     /locus_tag="PC429_002952"
     CDS             complement(1140..1676)
                     /locus_tag="PC429_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF3848439.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNGVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1749..2162)
                     /locus_tag="PC429_002953"
     CDS             complement(1749..2162)
                     /locus_tag="PC429_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF3848440.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2628..3245
                     /locus_tag="PC429_002954"
     CDS             2628..3245
                     /locus_tag="PC429_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF3848441.1"
                     /translation="MDEKILELLQEMQGSISGIEGTISDMQSNINGIEGTISDMQSNI
                     NGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISSMDGTINS
                     MQDSITDMQVEIKEVNSKLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINR
                     NTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3853..4032)
                     /locus_tag="PC429_002955"
     CDS             complement(3853..4032)
                     /locus_tag="PC429_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3848442.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNNYNIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(4258..4791)
                     /locus_tag="PC429_002956"
     CDS             complement(4258..4791)
                     /locus_tag="PC429_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF3848443.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPDFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGINRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            complement(6212..6784)
                     /locus_tag="PC429_002957"
     CDS             complement(6212..6784)
                     /locus_tag="PC429_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF3848444.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6846..7109)
                     /locus_tag="PC429_002958"
     CDS             complement(6846..7109)
                     /locus_tag="PC429_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF3848445.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(7109..7330)
                     /locus_tag="PC429_002959"
     CDS             complement(7109..7330)
                     /locus_tag="PC429_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF3848446.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            complement(7846..8382)
                     /locus_tag="PC429_002960"
     CDS             complement(7846..8382)
                     /locus_tag="PC429_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF3848447.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(8400..9713)
                     /locus_tag="PC429_002961"
     CDS             complement(8400..9713)
                     /locus_tag="PC429_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF3848448.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            10068..10508
                     /locus_tag="PC429_002962"
     CDS             10068..10508
                     /locus_tag="PC429_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF3848449.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(10693..10881)
                     /locus_tag="PC429_002963"
     CDS             complement(10693..10881)
                     /locus_tag="PC429_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848450.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         4040 a         1949 c         1301 g         4278 t
ORIGIN      
        1 atagcttgta gtgtacttcc ctcaagagga atagtcgttg aagtttctcc tattcggagt
       61 ttacctgctg attgcccatt ttaaagtact taggatttaa ccttatacca tctaaccaat
      121 tttttctact ttcgtaactt tcacacttag gtttatttca tccttatgtt ttagtttggt
      181 tagctttagg ggtttccagc aattcaagta gtattggata ggttctatcc tatctctaca
      241 tataggtttc cctatatgct gactatttta gctactaact catgactaaa gtcacaagtg
      301 tgcgtagcta ttttttaatc aaactggaat acagactcca ttcctaggaa attggctgaa
      361 atatagtctg ctgtcaaaga tgctgtcatc acagaaatta aaacagatac tgaaaagttt
      421 ttatggactt cttcaagtga aaacataaca cccgccagtg gtgcatgaaa ggctgctgat
      481 aatccagcac ttgccccaca ggttaacagg aatttttcct ctgtcttccc tcggtcaaga
      541 gctctagaaa tacctttacc tgccatagca ccaagctgaa tggaaggacc ttcccttccc
      601 aaagcaagac cacccatgag tgataagaat cctccaataa attttgttgg caacactcgc
      661 caccatactt gttttatttt ccctgtcatt tccccctcaa gctgagggat accacttcca
      721 gaaatcattg gctcccagtc caccaacttt gatacaatcc atgcaagtac tgcaagtact
      781 gcaaaccaag ctattatttt tattgtagaa ccttctacaa attttaaaat ttggctaagc
      841 caagtaccag cctgccctaa aagtatcctg tataaaagca caatcaaacc agccacagcc
      901 ccaaccataa gtccttcacc aatcaaaatt acctgaaagt gctctgcccg tttgataata
      961 tgagatgtat cctttttcat actgttcctc cttcatccaa taattctaca caatctgaat
     1021 ctccaatgac taaagtcatg agtgtgtaca gatatttttt aatcaacaaa aatagatttt
     1081 tatttactaa atataagaaa ataattgctc aatttctcaa taaatgtaaa tttattcttt
     1141 taggtattta ttctccacag tcgtgcttct tccagcaaaa tatttattag ttcttgcttt
     1201 ctctgtgtat agattttacg attgtcctca aactgtaatg ccaatttttg tttatgacta
     1261 tcatagagtt ttgctttgtc tgtaaaagta tttagataat ctcgaaaatt gatataatta
     1321 ttccatgcaa caccattcca ttcaacaaca tgaatatggt gagtacgagt atttttctca
     1381 aaatctccca tagcaaaaag taattgtttt ggtacatcct gacctcgaaa aacgattcca
     1441 cattgctcta acctttcgag gtataattta gcttcctcta aagaagcaac acctacaaca
     1501 atatcaataa taggctttgc gctaatactg ggaatagaag tgcttccaat gtgttgaata
     1561 tcaatggcag tcttatccca tatacttttc aataatataa tgacatcttt tgcactttta
     1621 tcccatatca tctgatgttc caataattta accataccac attttaaacc tatcactata
     1681 taccacctca atataatcca cctttatata tattacaatt ttttctctct tgataaacag
     1741 tgatttagct atttcacttt ttcttgacac tcgctacatt ccctatcatg aatagatata
     1801 attcctccac atttagaaca agtatatctc tccttctgct tttgcataaa cttctctaaa
     1861 ccgtactgct gaacaaattt actattttcc ataaggcttg cttgatatct cttgttataa
     1921 cttttttcaa gattttttat gagtttacaa ggaaaattga aacattcaaa acaataaaac
     1981 aacccttttt gacagacaca atcctttatt ttacacttac ggcagtgttc tggctttcct
     2041 gtatcgccat tcaaacaacc agcacatggc tttttgtgat aacaatgctt atagcaaacc
     2101 atacaattca ttccacaagg agcaaacata atcgtatcaa tactctcttt tggcattttc
     2161 ataacatttt actcctttca aaaatccaat ttgtcgaatt gttatatatt catattgtaa
     2221 cataaaaaat agctaaaaaa ttcatgcact ttcattgcca ataaattttc aaggaaatat
     2281 gaaagtttaa aactaagaat aaaaagagat aaaaaggaag ctactaatca aattattcaa
     2341 atacaagtaa tcaatataaa taacttaagg tatatttaaa agtatatgat attaccagca
     2401 gttttaaata caaatataac cacacatgcc tcaaaaacac ttattacagt atcttagaaa
     2461 cttagacaat acataagatt cattaataaa aaagttctca atatagatat atttaaccat
     2521 gttttgaata gcagataatc atatgattaa aaattaaata tgacatagcg tttgtggaat
     2581 ataacaccta aattatgtta taataataaa acaggagtgt gatagtgatg gatgaaaaaa
     2641 tattagagtt attacaggaa atgcaaggta gtatcagtgg tatcgaaggc actattagcg
     2701 atatgcaaag caatatcaat ggtatcgaag gcactattag cgatatgcaa agcaatatca
     2761 atggtatcga aggcactatt agtagtatgc aaggcaatat caatggtatc gaaaacacta
     2821 ttagtggtat gcaaggcact attggcgata tgcaaggtag tatcagtggt atgaatggca
     2881 ctattagcga tatgcaaggc agtatcagta gtatggatgg cactattaat agtatgcaag
     2941 acagtattac tgatatgcaa gttgaaatta aagaagtcaa tagtaagtta gacaatatgg
     3001 aacctgatat taaagactta aaaactggac aagttgaaac tagtagtaga cttgatacta
     3061 ttcagagtga tgttacagaa attaagtcag atgtaaaaat taacagaaat acattagata
     3121 cagtgattaa acatacctct tcattagtaa aagatacatc agatattaaa aatgatttaa
     3181 ataatataga gatagtcaca tctaagaatt cgaatgatat tgctaaatta aaatcagtta
     3241 aataatatac catagtaaaa aatacctgaa aatttcacat acttctttaa agttatacca
     3301 aattttatac agaaatactg attatccgac aacacacttt acttataaat aatttattaa
     3361 aaaaatactc attttatcct aaaattaaca cgactaaatg agtcttgata taataataaa
     3421 aaataaggga gattggacac taaaaagttg tgtatctccc ttatatccta taatatagtt
     3481 atataatttt tcagcctaaa ataaaagtcc atctttgatt aaaacacatc cccttatcac
     3541 ttataaaagg ataaaatctt gttttcattt ttccatttat atcttcttgc ttaagagtaa
     3601 taattttttc ttttgatact ttaagtgttt ggttaaattt aactaatcga ctaagttttg
     3661 ttcaacatct atttttcata ttttccaata cttaatctat tttttataaa taattctttc
     3721 atttctaact cacttacatt ttttacataa aacgaaacat ataaatttca accttatctt
     3781 gtatggttct taatacttaa tgagtagctt aagtatcaat cattataaat ttttccctta
     3841 catttaacac tcttaccatt tccaaaatat acaaaatata ttttgaacat ttgtacaatg
     3901 atgaaataca atacatgaaa atacaatatt ataattgttg tctaaaaata gaagttcttc
     3961 aacataacct ttcacaaaaa acatattatc taagtatttt ttgtctgctc cttgttttcc
     4021 tgtttgtagc atataaatta cttcctctaa acatataact actcacatga aaaaagaaaa
     4081 taaacttcca aaatacaagc tctaaaaact acctctaaag ttgtataatg tttactagga
     4141 ataaggttgt ataacatata gtttaaataa tcatttttta aaattgcaag ttattgcttt
     4201 caaaattaga tgtttgtata tcaaataaat gattaagaat ttctaaattt aatggcttta
     4261 taatgcactc cctttctttg ggataaaata atgttgcaca gatacttttt ctaaagataa
     4321 caattttatt tttctattaa ttccaccagc atacccagtc aaactcccat ttgttcctac
     4381 aacacgatga catggaataa taatagagat tccattatgc cctactgcac caccaattgc
     4441 ttgtgctgac attttagata ttcctttttg ttgagcaact ttatatgcaa tttctccata
     4501 agtgattgtt ttaccatatg gtatttgttg taaaattttc cacactgtag tctgaaaagg
     4561 agtacctacc atatggagtg atggtgtaaa atcgggttct tttcctgaaa aataaatatc
     4621 taaccatttc tttgtctgct cgaaaattgg caattctttt tcttgatgtt cttggtcaag
     4681 attataagca taatactttt gattctcaaa ccaaagacca gttaacccaa tattatctga
     4741 tgctaaaaga atatttccaa ttggactatg gtaatagcta atatattgca tacaattcct
     4801 ccttatattc tgaaaacttc actcaaatca ttagacactt cttttctatc taccattatg
     4861 taaaaacata ctaatataat ttccttctaa ctacactttc gttataatat ctatcctcat
     4921 atcatattag ttttatattt acctgcatct gttcatcctt ttttcccttt ccctttgatg
     4981 gatataaaat tcatacattt aagcttccta ctgtattaac ttttatagaa gtgtatctta
     5041 taattttatc actaaataga ccaaaaaaat atcgatatag catcaagtaa tatcataata
     5101 ctttcaaatg ggaaaagtaa gaataggctg taaaaaagaa aagtattttt atgttagaaa
     5161 tccagtaaaa tcgttagtag atagaataac agaaaacgaa caatttgcta tttatcattt
     5221 tacacataaa tataattgac gcaataaagt gttttctatt ctacaacgat taataaggtt
     5281 agaaaaaagt aaaaatgaac tgtatatgta tgaagcatta gtttctgttt ctttcctttg
     5341 gcttattatg ataaaaaata ttgttttgcc accaataaaa cacaaaaata taattaatat
     5401 ccatatgcaa aaaattttac gttatattga aaaatactat tgttttacat tatattgaag
     5461 ggtactattg tgaaaactta attttggagt actatcaagc agtggtaata tcaataaatc
     5521 agagtgctcc agatgatttg aactaagttt aaatactata ccttataaat attttactga
     5581 gttttgttta ttaaaagcaa ctcaatttct aaaaggcaca aatgagctaa taagtgacat
     5641 tactataaaa gttgtttttc atcaaataat ctattttagt aaatgtttta aagaaaaaac
     5701 gggatattct ccaaaagtgt acagaaatat aaaaaaagag atatgatttt ttatttcttt
     5761 tgattatcca aagagtaaat aaaaaatatt gtaaaaaact tactaacaac ggcaatgctt
     5821 tgaactatgg taatactttg aacttttgat tggacttttt ttgctaaaat aatatattta
     5881 aatagcaaag taatctgttt aatcaataaa aaataagaga taaaagaaat caactaaaac
     5941 ttttacctct tattttgata gtttatcaga gctaatattt acactctttt tatttttgat
     6001 acttcattaa aaatgatgct tattctcttt tcatttttaa tacttcatta gaacttatgc
     6061 ttattctctt ttcactttta attcttcatt agaacttatg cttactctcc attcactttt
     6121 aattcttcat taaaaatgat gcttactctc cattcacttt taattcttca ttaaaaatga
     6181 tgcttattct ccattcactt tcaattcttc attaaaactg atgcttgcac tctgtaaact
     6241 cataaacatc cattaatgct tcaccaaatc tttgatagtg aactacttcc ctctcaccca
     6301 aaaatctcaa tatatctttc aaatcagggt catcagttaa atttattaat tgatagtacg
     6361 tagccaatgc tttttgttca gcagccatat cctcatgtaa atcagttacc gaatcgccaa
     6421 agacattaat ataacttgta gtaaagttta ctccattaga atctactggc tgaagaccat
     6481 gtccaaaatt agcataatta ctaccaagtc cagcagcctt aagctcttct ggagtacaat
     6541 tgcctattaa ttgatataac actgaagaaa ttatctcaac atgagccatt tcctctgtac
     6601 ctatatcagt taaaagtgca cgagattttc cagttggcat agtatatctt tgttgaagat
     6661 atctagttgc agctgccaac tcaccattag gtcctcccaa ctgagtcatg atatattttg
     6721 ccattctagg gtctgcttgt cttatgttaa ctggatgttc cagtgttttt tgataaatcc
     6781 acatattatc ttactcctcc ttaatatata tttaataata aatctaattt taaagtttaa
     6841 taattttatt tataaaatga tttgtcccat ggccaaggtt cagatatcca tgaccaagga
     6901 tattcacttg attcatatcc aaaattagtt aatgctccat atttagattc ataatcacac
     6961 acagcttgtg taaatttatc gcagtatctg ttgtatgaat caagagcttt cttatccctt
     7021 ggattgttat ctaaatataa atttaattct atacaggcaa attgatattc tgaaattctc
     7081 tctaaaagtt ctcttctatt taatgacatt agtacatgtt accccctttt ttagctctaa
     7141 ctctattttt aggatttgca tatagccaat cattatagtt atttgactct attaaatcta
     7201 attcaggaaa taaagtacct ctttttaatg ctgtttttgg attgtatgtg tcatcataaa
     7261 tttgacgatt gatgtatggt ctagcaaatt cacaacaata gtcttgttta tcctctttta
     7321 aatatcccat taattatcct ccttaatttt tagaatttta aaatagcatt atttttaaaa
     7381 attgtatact ggttctatat tataactggt agttttaata ggtctttaat aagattaaat
     7441 gattaaataa gtatcttttt ttcttatttt acctctaaaa tttttctaat taatctttca
     7501 taaatatcta ttttaaataa ataatataga gtttatacaa ttttttatag cttaattact
     7561 atttactata tattattcat ttttaaataa aatgtgatta ttttttatta aaaacgatat
     7621 aaatatacaa tttaatacta tttttttata aataaacttg tattaaaata catttagtta
     7681 tataatttta ttttttctaa gagtataatt ataaaaataa aaaaagaata ggataatttt
     7741 cctactcttt tttctgtact tacatataat attaaaactc tttatttttt aatatatatt
     7801 ttaaattttc ttttttaata aatctttttc ttttaataaa tctatttaat tttcttcttc
     7861 tatatcagag ccaaagggaa tccatgcatt accaaactct ttatctttaa ataaataagc
     7921 taatccagtt atttgcttca atctaagaat ttcatcaact tccataccca ggtgctttga
     7981 tatccattca tcagattttc caatttcaca aagttcagtt ataattttac tcattaaatc
     8041 cacattatga gaacctcttg cacgattgtg tcgaatagta cttgccattc tttcatcaag
     8101 agatttatca attactgaaa caggaagaag tccattttct ctatcatata tatcctcatt
     8161 ttcaagcatt actctatatc tatgaaaacc atctacaata atatacttat ccttttcctc
     8221 attataataa cagacaattg gcattgtata cccatcttct tttatacttt tatacaacaa
     8281 ctgcatttct ggtggggcta ctgcatttgg attatagtcg ttagattcta tcttttcgat
     8341 tggcactgcg atgattccat atacaggact tttatattcc atagcatctt aactcctatc
     8401 taatgaattt tttcatattt cttcttaata tattccgttc taattctctg ttcttttgta
     8461 ggaccaaatc ccataaatct acacatatgg tcatttttta aaatacaata acacattctt
     8521 ttccaacttg gtatatcttt ggtaaattta atatcatccg tatcatcagg tatatcatcc
     8581 aaaaatatga ctctactctt tttatctttt gtatgatttg aaactccatt taattctatt
     8641 ttataacctt ttctcaacaa ttcacgtata actatttctg acaaaccacc acctgtattc
     8701 tgccaaaact caatagatgt tgaaaatttt ttaatatagc tattccttat attttcaggt
     8761 aaggtatcaa gcaaaaaata tgtgaaactt ttccatgtgt gtccatatgg caatttaaca
     8821 ctacgatatc ccatagcttt tgttttgcca taaatagaag taaaattaac tccttgtact
     8881 ctagcaacca atttcaccca tacatctggg tccaatattc tgtataaatt taaactctct
     8941 tttgctgaat cattaaaagg tgatgccact ctcatttttc ttggagctac tccagcttta
     9001 tagaataagt cataaatttt attatagtca tagtcaaatt tatagtgtgc taaccatata
     9061 tcttctattt cccaatcata tataggagat gccgtccaga catttttaaa ttccttagtt
     9121 atccagcact ttcctttata tccatgctgt ttattcacaa tagaacaata acgttgtaga
     9181 gattctgctg ctcgtgttcc taataagcaa acaacttttg caccttcatg tatatctcta
     9241 atccatctac caaattgttt tgccaaactt tcttgtctca ttttatattt ataataagta
     9301 aaaggattat ttttttgatt tattatgtat tctttgactg gcatttccct tacccataac
     9361 tcttctttct catcatccca tggataccaa tacatttcat aatttcctac agcagttctt
     9421 gtagccattg gtaaacacac ccaatatggt tcaatatctt caatattatc ttcaaatact
     9481 ttttcaacat attcagtagt caaactatat tgagcctcaa aatcttgatg aaatactcct
     9541 attttggaac ttatattatt ctttctctta taatccaaaa ctaaatttag cagtaaacca
     9601 ctatcttttc ctccgctaaa agatacatag atataatcaa attcattaaa aagataagaa
     9661 agtctttctt gaaaagcttc aaatacatcg gattctaaat atctcttatc catttgaatt
     9721 atcccctttc aattagaagt ttataaaacc ttacaatgca atattattat gcttttttag
     9781 cagtaatcag cataaattat acattattta aatacacaaa tccaactaac tacatataat
     9841 tataatgtaa ttatactttt gtattataaa ctatatttta tcattatttt acattttttt
     9901 ccaatatata taaatatcat atttttctat tagctaaaac taattacttt atatttctac
     9961 gtattagtat aataatacat aaaaacttga ataatgcata aaaacttgac atactgttgt
    10021 cataattact ttgctataat taaaagtaaa aatggaggta aattctaatg tatgaacgta
    10081 tgcttgataa aagcaataaa ccctgtttgt ctcaaataat agaatatata ggtaaagatg
    10141 gatatgcacg attgtgtaag ttggaaactt acttgaaaac tcaatataat ttatcaagag
    10201 aacttcgatt tccttttgga aacaattatg gttggggtta caaatatagc cataaatctt
    10261 ttcatttatt ttatgctttt tttgaaaaag gtgcatttac tgttctgatt cagataggtg
    10321 acaagcaagt tccagcagta gaaaaaacgt tggtagcttt gacaccaaaa gctaaagagc
    10381 tctgggtcac tcgttatcct tgtggcaata atggaggctg gattaagtat cgtattttag
    10441 aagatgcaga cttacttgat attattcaat atataagagc aaaaaaatct ccacctaaac
    10501 aaaattaact tttatttttt gtgatttaaa tatagtgtat ataaattcca ttctttccat
    10561 ataaaaaaaa taatataaaa aatatctatt agtataactt atttagtatg tcttattcta
    10621 ctttttacag ataattaaaa agttttatca taaataaatc ataactagac atcctagtaa
    10681 atcaaaatta atttattctt ttttattaga atttttgata acagtcccta tacatatgcc
    10741 aattatcatt ccaaaaatta gaccataaca cattgtgata cctacatttc ctttagaaat
    10801 acctatagca caacctatag ccattccaag tatcatgcct ataaacatac atataataac
    10861 taaattgttg tttttattca tagttttctc cattcaatat attttaaatt gcctagctcg
    10921 ccttcttagc ttaaaacaaa gaaatataaa ttaactttct ttcattctaa tatctgcttg
    10981 tataaatgtc aaacaacaca tttcttgtca taacctcctc tttttgaagt aagtacaatc
    11041 tcccagacgc aataatatca atttcctaaa accatttaag taacttttat atttacataa
    11101 tatttacaat cataaactat atttttattc atttatacat cataaacact ccctactaat
    11161 cattattata cttaaaataa tgttcattta taataatata tctaacacac tttaattgaa
    11221 aaaaataaaa ctttgccttt cattcccata tataaataag tatacttttt gatgctctat
    11281 tacaaatcaa ctaaattaat tgatataaat tatcattttc tgttgaaaat accattcgca
    11341 catgctatac ttaattgcat aaatggtaat aaatgtcata tgattttaca aatctattaa
    11401 taacaattat tacttttact gtattttaaa ttttttggac taaatatcaa gaactttcta
    11461 aaatacaaag tacaaaaaat ttatataagt tataaattta tatgaactag ataagctcaa
    11521 acaatatgtt atgtataaac tgtgtagtat tcggttataa atttatat
//
LOCUS       DAMKMI010000124        11540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMKMI010000124 DAMKMI010000000
VERSION     DAMKMI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.129"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            90..980
                     /locus_tag="PC429_002964"
     CDS             90..980
                     /locus_tag="PC429_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3848451.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            1198..2952
                     /locus_tag="PC429_002965"
     CDS             1198..2952
                     /locus_tag="PC429_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF3848452.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            3336..4322
                     /locus_tag="PC429_002966"
     CDS             3336..4322
                     /locus_tag="PC429_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF3848453.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            4491..4639
                     /locus_tag="PC429_002967"
                     /pseudo
     CDS             4491..4639
                     /locus_tag="PC429_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            4658..6139
                     /locus_tag="PC429_002968"
     CDS             4658..6139
                     /locus_tag="PC429_002968"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF3848454.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            6165..7136
                     /locus_tag="PC429_002969"
     CDS             6165..7136
                     /locus_tag="PC429_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF3848455.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(7392..7994)
                     /locus_tag="PC429_002970"
     CDS             complement(7392..7994)
                     /locus_tag="PC429_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF3848456.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            8275..9486
                     /locus_tag="PC429_002971"
     CDS             8275..9486
                     /locus_tag="PC429_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF3848457.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            9554..9751
                     /locus_tag="PC429_002972"
     CDS             9554..9751
                     /locus_tag="PC429_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF3848458.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            9785..10756
                     /locus_tag="PC429_002973"
     CDS             9785..10756
                     /locus_tag="PC429_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3848459.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            10943..11320
                     /locus_tag="PC429_002974"
     CDS             10943..11320
                     /locus_tag="PC429_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3848460.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         4735 a         1155 c         1888 g         3762 t
ORIGIN      
        1 aaataaaaat atattataaa aacaatttga ttggaatttt cagaaaatta atgctataat
       61 taaaagtaaa tttaaaggga ggttataaaa tggctaaatt aactctagaa catataaaag
      121 gaaatacata ttatattcca ctccctacaa tagtaggtgt atacgtagat ggtaaggatg
      181 caattttaat tgacagtgga aataataagg atacagctag acaagtttta agattattag
      241 aagaacataa tttaaatcct aaattaataa taaacactca ttcaaatgca gaccatatag
      301 gaggaaatgc atatttgaag aatcaaacaa aatgcaagat tgctacgact aaaattgaag
      361 gatattttac agaaaatcca atattagaat cagcattttt atatggtggc tatccaagta
      421 aagcactgaa gaataaattt ttattagcaa aggaaagtga agttgattat attatacctt
      481 caaatggcaa aattgtagat acagaacttg aagcgatttc tcttccaggt cattattttg
      541 aaatgattgg tgttagaact ccagataatg ttttgtttgt tgcagatagt cttacaccag
      601 agaatataat tactaaatat cattttttct tcctactgga tatagattca cagtttaaga
      661 cactagaaaa attgagaatg ttagaggctg atttctttgt tccaagtcac tctgtcaaaa
      721 ctacagatat aaaaagttta attaatatta ataagaagaa gatggaagaa ataatagata
      781 atattaaaaa ggtttgttat gagccagtta tgattgatga agttattgaa aaaatgtgtg
      841 atttgtacaa tgtcaagctt gatgctaatc aatatgtgtt ggttggaagt acaatacgtt
      901 cttatataac ttatttatat gagaataata tggttgaata tatatttgat ggtggtaaga
      961 tgatgataaa agctgcgtaa atagtaataa ttttcaaatt atatgatgaa attgttttag
     1021 tatgactgtt atttgaaaag taatataaaa aaatttatat aaataagcta agatgattga
     1081 tataaagtta ctgataattt taaggtaata tatacgttac tataatatta tgttattatt
     1141 tgtaaataag gggataattt ataagcaaat taaagacctt aatttacagg aggaatagtg
     1201 tatattatga ataaaaaatt caaaaaattc agtaaaaaac taagtatatt aattttagtt
     1261 ttgactatgt ttctttttaa taatttaact gcaaatgcac agattaaaga agattcaata
     1321 ataggaattg atagatatga aacagcagga cttatagcgg atagacaaac ttatgatact
     1381 gtaatattag ttaatggaga taaaaattta tctgatgggc tttcatcaag tggattagct
     1441 ggagctatta acgcaccaat acttttgact aaaaaaagtg aaataccaaa agctacttta
     1501 aatagactag ataataagag tttaaatgtt gtaaaaaaag tatatataat tggtggttat
     1561 aatactattg aaaattcagt agaaaaagat ataaaaagaa aaggtattca agtggaaaga
     1621 ataaatggta acaatagaat tgaaactagt tataatgtag ctaaaaaaat aaatgaagta
     1681 agtagtgtaa aagaggtaat gcttacaaat ggatttgtag gagaggctga tgctatgagt
     1741 atagcacctg tagttgcaaa aaataaagga gctataatac ttacagatgg gaaatcagtt
     1801 ccatttaaga ctgaaggttt aaatgtttac gctataggtg gcaaatctgc gataaatgag
     1861 gatttagtaa atgaaactaa agcaattaga atcggaggaa atgatagatt tgagaccaat
     1921 aaaaaggtaa tggaaaagtt ttataatgga gctacagatt tttatataac aaatggatat
     1981 caattagtag atgcactaac attatctcct ttagccaaag aaaaaccaat cgttttagtt
     2041 gctgatggaa gtaataaagg tattttaaaa ggagctaaaa gtataacaaa agttggaggt
     2101 attgatgcca atatatataa acagtgttta gatatgatta aatataataa tatggatatg
     2161 actccaaata ttttaaaaca cttaacttca gttgaaggaa atgaagtttc tgaaatactt
     2221 atagaatcag ataatttagg tgtggttgtt gaaaagacga ataccaataa atttgagttt
     2281 gattacgtaa gtgtaactaa tgaaaaaaat tgtacttttt ctgtatataa agaaaattct
     2341 tctaataata taaaaaatgg aaagctagtt gtaagtgcta agaaaaaaat agataagaaa
     2401 gatagtgatt tgcaagatat gaatggcgac aatatgataa atgcaaataa agataggatg
     2461 gtaaatgtga taaaaattgg agttcccgat aaaatgtatt ctaattttaa agtagatgtt
     2521 gaaagtggtt cagtagaact ttataatatt aaaggaggag ctactgttga tgtcaatgac
     2581 ggaattgcta acattgttga taacaatgta acaaatcctt tcaatataaa tactaatagt
     2641 gggatttcag gtgtaacagc tgaaactata tcatctgaaa taaaatttag agcaaacaat
     2701 ggaattgtaa atgtaacagc tacaaatata aatggcaata taagtttatt tggaagcgat
     2761 aataaagata cttttgatgg aatatttaaa ttaaatctga aaaaagaacc atcaaattta
     2821 cacttgaagt taattggaaa tgggataaat aaattgccag aaggttggtc taaagattat
     2881 attttaggaa atggacaacc aattattgaa gttaaaaata atggcataaa taatatatca
     2941 attggagaat aaaaacatat gaaaataaag tttatatagt tttaaaaatt atagaatttt
     3001 aaaatattta tatatattct ttaaataaaa aattattttt taagtgataa ttaagtgttt
     3061 atcgataagg ctatggttgt aaaaatcata gccttatttt tatattataa ataaattaga
     3121 gtatttatca attcatagta gataaaattg aaattaaata taacaacaaa aaatgaaaat
     3181 tacaattaat ttaatgttaa agactcaatt atttggatgt ggaatggata gtacaagtag
     3241 tatataaaaa gtattgactg gacaaataac tatattttat tttaaatata taatataatg
     3301 taataaaata tatcaaatgt agaaggtgat aacctatgaa aaaaataaca ataaatgata
     3361 ttgctaactt ggcaggagta tctaaaagta ctgtatcaag atatctaaac aataaagata
     3421 tcagtgattc tactaaagag aaaataaaga caataataga tgaatatgga tatgagccga
     3481 atgcatttgc tcaaagtttg agagctaaaa aaacatattt tattggtatt ataacaccgt
     3541 gtttagattc ttttgtaaaa tctaaaatta tgatggcaat agatgaagaa ctcaaaaaat
     3601 taaaatatac atcactaatt attaatacta atagaaaaat aaggtcagaa atagatagta
     3661 tatcaaagtt agctagtcta aaagttgatg gaataatact aattggtacc gaaataacta
     3721 aagaacataa gaatgaaatt gaaaagttag atataccgat agttgtagtt ggacaaaaag
     3781 ttgatggtat caatagcata gtgaatgatg attatggagc aggctataaa atgggtcaat
     3841 atattgctaa taaaggttac aaaaacattg tatatttagg tgttgatgaa agtgatatat
     3901 cagttggttt aaatagaaaa aatggagtac ttaatgggtt aaaagataaa gggtatgatg
     3961 caaaagtatt ttatactgac tttgaccaag aaatgtctat acaaagaagt ggagaaatgc
     4021 tagaaagtga aaatccagat ataataatat gtgcaactga taatatagca atagcaacaa
     4081 tgaaagaaat caataagaga ggtaaaagta taccacaaga tatatctgta gctggatttg
     4141 ggggatatga tgtattgtct attataagcc ctaaacttac aacaataaag tttgaaaata
     4201 aaaatgcagg taaagtagca gcaaatacga tagtaaattt aatacaagaa agaaaagaac
     4261 cactattaaa ggagattaaa tttgaattaa tagaaggtga aagtacaata aataaaaatt
     4321 aagatacaca ttacatatat tagtaatgtg tattttttta ctaaaaaaac tataattcta
     4381 aaaattagga aaactttttt gaaagaaata ttgcatctga aataaattta tgctaatctt
     4441 taattaaacc ggtttcgaaa ccggtttaat taaaccgata gaatagcaaa atgagtgcaa
     4501 aaataactga ttgtaacaaa gtatcaaagt aattattgga gtacatagga ataaacaaaa
     4561 tataaaagat gtagcacatt gtgtaataag actaagaatt gttttagata gcaataataa
     4621 agcaaatata aaattataaa agaaaaggaa taaaaatatg tacaaagagc ctaaatatag
     4681 aaccatatta gaatcgacaa aagaagaatt aaataaatta agaaatatat ctttaaatga
     4741 taaatataaa ccactatttc atatacatcc acaacatgga ctacttaatg acccaaatgg
     4801 attagcatat tataatggta agtaccatgt attttatcaa tggtatccat atgatgcaac
     4861 tcatggaatg aagcattggg cttatgtaag ttctgatgat tttgtaaact ggaatagaga
     4921 agatgtagca ttaataccaa tcgaaagtta tgaatctcat ggagcatatt caggtaatgc
     4981 gatagaggta gatggaaaat tgcatatgta ttatacaggg aatataaaat atagtgcaga
     5041 agatagatat gcatatcaaa acttagctat aatgaacaag gatggtaaaa taactaaata
     5101 tgaaaataat ccaatagtaa gtgaaatacc aaaaggatat acaggacatg taagagaccc
     5161 aaaagtattt aaaagaaagg ataaatactt tatgttactt ggagcacaaa caagtgataa
     5221 aaaaggagtt ataatagtat atgaatctaa aaactctata gattggaatt ttaaaggaga
     5281 attaaatgta aaaaacatag atgaagattt tggttacatg tgggaatgtc cagattatat
     5341 taatatagat gaaaaagata tattaatatt ttcaccacaa ggagtagaac ctaaaggatt
     5401 tgattatcaa aatatatata atgtagtata tgctattggt aatatggatt tagataattt
     5461 aacatttgaa atagatacaa tgaaagaact ggaaaaagga tttgatttct atgctccaca
     5521 aacatttata aaggactcac aaataatact atttgcatgg gctggaatgg gagaagtttt
     5581 ataccctaca gataaaaaca aatgggcaca ttgtctaact gttccaagaa aattaaatat
     5641 aaaaaataat aagctattgc aaatgccagt agatgaatta attaaattaa gatatgatga
     5701 aacaagtggg caaaatacta taaagaataa tattaatatt atagaaaatg atgaaaatgt
     5761 atatgagctt aatattaata taaaaaatat tgacagcaat aaatttgggc tggaattatt
     5821 ttcatcacaa gatgaaggtg taaaattaga atttaacaaa ttaggaaaca ttgtaacttt
     5881 agacagaagc aactttaaaa aagttttcgg tgttgaatat ggaacaaata gaaaagaata
     5941 tataaatatt gatgaaaata ctaatatcaa agtactagcg gatagaagta ttttagaaat
     6001 ttttataaat gaaggagaag tagtatttac tagtagaata tttgctaaag aaaattctaa
     6061 tcaaataagg gtttatagtg ataaaatagt agagtatgag tatactaaat ttaaattaaa
     6121 gcaaggtatt gaattataaa aacacattat aaggggtaga aaagatgaaa aaagtaatta
     6181 gtataggaga agctttaata gactttatac ctaatcaaaa tggtggtaaa ttaaaaaatg
     6241 tatcagaatt tagaagagta gcaggaggag ctccagcaaa tgttagtgct gttgtagcaa
     6301 agcttggagg aaaatcgagt tttatatcta agttaggaaa agatgcattt ggtgattata
     6361 taatagatgt attaaatgaa gtaaatgtaa atactgatta tgtattgaga acaagtaaag
     6421 caaatacagg acttgctttt gtatcattaa aagaagatgg taatcgtgat ttctcatttt
     6481 atagaaatcc aagtgcagat atgcttttag aagctgatga agttaaaaaa gagtggttta
     6541 ataattgtca tatcctacat ttttgttcag tagatttaat agatagtcca atgaaattag
     6601 cacataaaaa agcaatagaa tacgcacttg aaagtaatag tataataagc tttgacccta
     6661 atataagatt acctttatgg gatagtgagc aatcttgtaa aaaggcaata tcagaatttt
     6721 taccttttgc tcatattgtt aagatatctg atgaagaatt agaatttata acaggagaaa
     6781 atgatataga aaaatcatta cataaactct ttgtggggaa tgttgaacta gtattatata
     6841 caaaaggaaa agatggagta gatgcataca ctaaaaaagt aaaaggtatg tgtaaagggg
     6901 ttaaagtaaa tgcaatagat acaaccggag caggagattc ttatataggt tcatttttat
     6961 acaccttatt gtataaacaa ataacattga atgatataaa agaaatgaaa caagaaacgt
     7021 taaatgagta tttagaattt tctaattatt atgcagctcg tagtacaact attaaaggtg
     7081 cgataagttc atatgcaaca aaggaagaaa taactgaatt tataaaaaat ttatagtagg
     7141 aatgagtaat aggttttgaa ggaagtaaca tgtaaagagt ttttaaggat gtttactatg
     7201 aaaataatta acatataaaa agcaataagg aggctgtctc aaaatagaga taagcctctt
     7261 tatcattgtt gaaatgataa aaaatatttt aattattgtt actttttaga agaatatata
     7321 tcttatttta aataagcaaa ttatgtaaga tttatttata ttatcaatac tttacttgga
     7381 gtattatgtc tttagcagtt atctaattca attgaattag ataaaatata ataaatttgt
     7441 tttgcaaatt gacgagaatg ttcttctttt tctttattca taaaattagg tttacctaaa
     7501 tcacgttcaa caagcattcc tatatatttt aactgtgaat gcttacagta gcgcttcata
     7561 ccattttccc aaatatcagc acccttttca ggtctatatc cacatgttgt aataagtgct
     7621 actgattttc ctgcccatag agcggggcct ttctcattac catagtattt attcattcca
     7681 tacaccaatc tgtcaagtag tgctttcatt ggagaagtgc agtaccaaga ataaattgga
     7741 gtagcaaaaa caattaaatc acacataaga attttcttga aaattttctg aacatcatct
     7801 ttacaataac atccaaaatc aaataagtta tcctgacact ttttacaagc aatacatggc
     7861 tctattttac aatcatataa ccatattact tcaatatttg aattataatt ttctaattcc
     7921 tcaataaaag gttttaaaat agcagcagta ttaccttttt tcttaggact tcccataaga
     7981 atgcatactt ccatattgat acctccacag tattttaaaa tagtttttag tgcttaatat
     8041 agttttagga tactatgaga atacgttaac aacaatacac tttgtatatt tgtaataaaa
     8101 ttgaaacaaa ttgttttatc aataaaagtt ggaatgtatc tataattaaa aatattttag
     8161 gtatgttttt acatatatgt gtgtagttaa ctatggtaat ttattgtaaa attatcttat
     8221 aggattataa ttgcttaatt tatgattata tttaaaatct tggaggtaaa aaatatgtac
     8281 tcaaatgaag actataaatt tatagaagaa aatgcaccat gttatatata tgatgagact
     8341 aatataattg aaaaatgtaa ggaattgaaa aataatattt taggttttga ttttttatat
     8401 tctataaaaa caaatccata taacaaaatt gtaaaaagca ttgcagagca aggttttgga
     8461 gcagatgctt cttcaactaa tgaagttatg atttcatcaa aatgtggagt ttctaaagat
     8521 gatatttatt attcatcacc aggcaaaaca gagattgata tagaaactgt tttagataaa
     8581 tgcataatta ttgcagatag tattacagaa atagaactta taaataagat atctaaaaag
     8641 cataatcaga aagttaaaat aggaataaga ataaatcctg attttactat ggatgatgat
     8701 agtgctaaat caagcaaatt tggtatagat atagaagata tgagcagtgt ttttaagatg
     8761 ttaagtatgt gtgattatgt atctgtagtt gggattcata tacatattaa atctcaggta
     8821 ttggattttg aaaagttagg acattattat gaaaaatgtt tttttatagc tttaaattta
     8881 aataaaagta agtatattaa tattgaattt ataaattttg gtagtggact tggagtagct
     8941 tatgataatc ttagagaaaa tccacttaat cttaaagagc tttcaaaatt aactcaaaag
     9001 attgctttta ctaattcaaa cacactaaaa tcaagacttc ttattgaatc aggacgtttc
     9061 atagtttgta aatcaggtaa ttattatgtt aaaattgtag atattaaagt atcaagaggg
     9121 aaaaaatatc ttattgtaaa aaatggactt aatggattta tgagacctat tatttctaat
     9181 ttaattgaaa atattacttg tggagtagaa ttagaaggtc aagagccatt atatacatca
     9241 aatcatgcct tttctctttc agtattaaat aatttaaaag aagaagagta tgtaaatgtt
     9301 gttgggaatc tctgtacatc attagatgta attgcaaata atgttaaatt gaaaaaagca
     9361 aacataggag atttgatttc aattagtaat gctggaagtt actcctattc tttatcaccg
     9421 ctattatttt caagttatga atttcctaaa caatattata aaagaaaaga tggatatatt
     9481 gagtaaacaa tagcataaat taataaactt agtattattg aaaaaattta aaatgcaggg
     9541 ggtgtaattc tatatggaat tgtggaaaag aaatttattt gtatgttgga taggtatgtt
     9601 ttttagttct atagggatga gccaaatagc acctatactt ccattatata ttaaacagtt
     9661 gggggtaact gatgttagtt taatacaaca gtattcaggt ataatttttg gatgtacctt
     9721 tgtagtagca gcattctttt cgccaatttg agggaaagct gctgataaat atggaagaaa
     9781 atcaatgctt ttacgtgcaa gtattgggat gggaatagtt atatttacca tggcgtttgt
     9841 tcaaaatgtt tatcagttat taggattaag aatacttcaa ggagttttta cgggttatgc
     9901 tactgcatgt actactctta ttgcaacaca gacagataaa aatcattcag gatgggcttt
     9961 gggtactctt gcaactgcat caactactgg ctcattaatt ggtccaacag taggaggata
    10021 tatagaatca atcttaggtc ttaaaagtac atttattata acaggaggat tgttgtttgt
    10081 atcatttatc atctctattt tatttgtagt agataatttt aaacctaaag aaataaaaga
    10141 atcaatttct atacctaaag aacaattaaa tatattgcca aataaatttt taattgcatc
    10201 tatttttgta accacattca ttactcaact agctttatac tctatagaac caattgtaac
    10261 catatatata tcacaactta caaattttgc atcaaatgta gctttaatag ctggattgac
    10321 attttctgca tcaggattag caaatttatt agcagctcaa aaacttggaa aaatgtcgga
    10381 taaaataggt cctcaaaaag tattgttaat ttcactttta tgggctggga ttatatttat
    10441 accacaagct tttgtaaaaa ctgcatggca actgatgttg ttgagatttt tattagggtt
    10501 gtcagttgca gggcttaatc cttctgttaa cagcttgctt aaaaaaattg caccagaaga
    10561 atatgtggga aaaatatttg gatataatgc atctgcccag tatataggct gttcatcagg
    10621 agcattttta gggggacaaa tatcagcaca tttaggtatt agaacagtat ttttttcaac
    10681 aagtttattg ttgtttagga atgcactttg gatgtataaa tttactggat tgtttattaa
    10741 agataagact aaataatcat actatttaca tttctaattt tatgatgtaa aataaatatt
    10801 ttttatgtaa tttatcatag aattatataa aaaataaccc tatggtaaaa attggaaaat
    10861 attattatcg acaaaatctt tgacttaaaa actatattgc gatagtatta atttaataaa
    10921 agtttgagag gggcgtaagg ttatgacaat ttcaaaaata ccacaagcag aattaaaagt
    10981 tatgaagttt atttgggaaa aaaatgatac agtaacatca aaagaaacta ttgaagctat
    11041 ggaacgaaaa tatggttgga aacaaacaac tacattgaca cttttatcaa gacttgttaa
    11101 aaaacatttt ttaagtgcag aaaaaataga tagatacact cattatacaa tagttgttgg
    11161 acataaggag tatttaagtg ttgagacaaa agatttttta agcaatatac acgacaattc
    11221 tttacagagt ttaatttcag cactacatga tgatgaagtt gttgataaag atatgttgga
    11281 tttatttgaa agtcatttta aaaatttaga agaagagtaa ttatatcagg atagaactct
    11341 aagaatttaa ttaatctatg gctgaaaacc ataaatggta ttatttaatc agctactcac
    11401 tattcataaa gtaatattaa ataggttctc taattagtaa aatgcctaaa ataattggaa
    11461 tagggagtat caagttaaat gataccactt atttaaataa gaaatgattt ttaaaattta
    11521 aaataatttt ataacttgtc
//
LOCUS       DAMKMI010000125        11415 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMKMI010000125 DAMKMI010000000
VERSION     DAMKMI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11415
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.130"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            55..240
                     /locus_tag="PC429_002975"
     CDS             55..240
                     /locus_tag="PC429_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF3848461.1"
                     /translation="MFFISYIYTKSPIKFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCVQINKKLNMKMVI"
     gene            complement(305..3079)
                     /locus_tag="PC429_002976"
     CDS             complement(305..3079)
                     /locus_tag="PC429_002976"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF3848462.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGIIATLLCMFMIIQM
                     QRKGMLILDTSSILSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(3251..4507)
                     /locus_tag="PC429_002977"
     CDS             complement(3251..4507)
                     /locus_tag="PC429_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF3848463.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSTVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(4579..6081)
                     /locus_tag="PC429_002978"
     CDS             complement(4579..6081)
                     /locus_tag="PC429_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF3848464.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            complement(6172..7362)
                     /locus_tag="PC429_002979"
     CDS             complement(6172..7362)
                     /locus_tag="PC429_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3848465.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            complement(7601..8689)
                     /locus_tag="PC429_002980"
     CDS             complement(7601..8689)
                     /locus_tag="PC429_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848466.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            complement(8974..9672)
                     /locus_tag="PC429_002981"
     CDS             complement(8974..9672)
                     /locus_tag="PC429_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF3848467.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            complement(9786..11291)
                     /locus_tag="PC429_002982"
     CDS             complement(9786..11291)
                     /locus_tag="PC429_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3848468.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTIPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTVKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
BASE COUNT         3716 a         2052 c         1296 g         4351 t
ORIGIN      
        1 ccttatgata cactataact ataagttttt aaatggaaag gaatgaaata aatcatgttt
       61 tttatcagtt atatttacac aaaatcacct attaaatttg atactccttt gcaaaaagag
      121 gcatataaaa tattacaaaa attagatatt gactttgaat gtgttgacac agatgaagca
      181 attacaatgg aagattgtgt tcagattaat aaaaaattaa atatgaaaat ggtaatataa
      241 aacaacatta aacaaacaaa aaccgcaaag ttacaatata tagtacttta cggtttcata
      301 agaattaatt tttactatta aagttcatag ttctactatt tttcattgtt ctaagaatta
      361 gctttacaaa ttcatttgac aataggatag tggatgcaaa tagtattatc tttatccaca
      421 tatcaaagtc caaagaaaca gaattaaaga aactttggaa cacttgtgta aatattattt
      481 gaattatccc tgtaactact attatttgca atgccagttt atttttaaag aaatttggta
      541 ttgtactatt taaaccaaac tctctacaat ttaatgcatt aaaaagggca ctgaatgcaa
      601 ataaagaaaa tataacagta ccttgttctt ctgatgtagc tcccaatata ttaaaagctg
      661 attgagtaaa tacaattgta attataagaa ttgcatttat aataatattt acaaacatag
      721 acctagctat tattccagaa tgtctattta ttggttttct tttcaataca taatctctaa
      781 caggttctaa tcctaatgct agggctggag gaccatccat tattatattt acccataata
      841 attgtattgt agtaaatggc atatccttac cagtaagttg agatattata gcaattataa
      901 atgctactat atttacagta agctgaaact gtataaatct ttgaaagttc tcatatatac
      961 ctcttcccca ttttattccc tctactatag tactaaagct atcatcagtc aatattatat
     1021 ctgctgcatt tttagatact tctgttcctg aaatacccat tgcaattcca acatctgctt
     1081 gactaagtgc tggggcatca tttataccat cacctgttac agctacaact tcgccacttt
     1141 tttgaagtgc tgaaactatt ctcatttttg tatctggttt acttcttgca acaatagata
     1201 ttcctttgat ttcttctctc aattctttat ctgtcaatac atctatatat gttgcttcaa
     1261 ctgccttttt cccatcattt aataatccta attcatttcc aatagctgtt gctgtattta
     1321 tattatctcc tgtaagcatt tttacatcta ctcctgcatt aaatgcttta tcaatggaat
     1381 ctttgacccc ttctctcaat gggtccacta ttcctacaaa tccactaaaa actaaattat
     1441 cttccttcat ataacttctt gaatttccta ttaaattcaa ttcaccttct gaagtcattg
     1501 caacttctgt tttagaatta ctcatttttt tataagcaaa tcctaatgtt ctcatagatt
     1561 taatttgtaa cttcttaatc tcatctaaaa tacttttttc tacttttgga gttattggaa
     1621 caatattttt tccttgttgt acataagaac atttttttaa taatacttct ggagcacctt
     1681 ttgaaagtaa tatatctgaa tcctcttgac gaattaatgt tgacatcttc ttttcttctg
     1741 aactaaatgg tatttgtgat atcaaatacg tttgttttct catttcatta tagttcttat
     1801 cattatgata taaaagtaat gcacactctg ttgcgcttcc tatatattta aatgaattat
     1861 cttctttttc tatatctgca gttgaattta ctatacagtt ttgttcaaaa tagctattac
     1921 tttgatactc tcctccactt atatatttac catcaacata tgctacctct accatcattt
     1981 tgttttgtgt taaagtccct gttttgtcgg aacatattac agaaacagaa cctatagttt
     2041 cacaggcttc tttctttgtt actaatgcat ttatctttgc catcttttgc attgttatag
     2101 ctaaggtaat atttatcata gttggcaaac cttctggaac agttgcaact attaatgcaa
     2161 tacaaaccat atatgcattt ttagcaggtt ctaatgattg taagaaagat aatatactgc
     2221 ttgtatctaa aattaacata ccttttctct gcatctgtat aatcataaac atacacaata
     2281 aagtagctat tatgcctgat attcctgcta ttttagcacc caacttccct aatttaattt
     2341 gaagtggagt ttgctggtct tcatttgata aattctgtgc tatcttaccc atctctgtct
     2401 tatcaccagt atgagtgaca accattatgc ccctaccata tgctacaaga gtccctccaa
     2461 ataccatatt gacttgcttt gcaggtattg catcctgttc gattatttct gttttagaat
     2521 aaactacttc catatctaat acagcatcag cattcttagc tacatcatca gattctcctg
     2581 ttagcatatc ttctctaagt ttcaaattta tagactgtat aagtcttcca tctgctggta
     2641 tcatatcccc agtttctata tatacaatat ctcctggtac taaatcattt tttgatattt
     2701 taattatctt cccatttcta agcaccttta cttctatatt ttctgttaat tttgacaggg
     2761 catgagctgc ttttttagat tttccttcag ttattatgcc tatagaaatt ccaataaaaa
     2821 tagcccctaa aatacctaat gcgtcatgta attctcccac aaaagaactt attactgctg
     2881 ctcctatgag aattaatatc ataggttctg tcaaactttc tcccaactcg tcccaaaatg
     2941 ttctaccttc ttttatagta aattcattta atccatatct aagcttcctt tcttctacct
     3001 cattatcatc aagtccaatt tctggatttg tttttaagta ttttaatact tctttggtag
     3061 gtttattata atacctcata actttatccc ccttgttaat tatctataag gaattatatg
     3121 cttatgatag cttgtatatt acttttcaaa aaaaataagg tgttgattaa tcaacacctt
     3181 atagtggtta tatattatta aattttatgt tacaggcttt ataaattgtg agctatacaa
     3241 atcaaaatat ttaatcttca aaataccctt ctggatataa tggaaactta gatataagtt
     3301 cttgtgcctc tgctttacat tcatctaata cttctttatt atcaatattt tctgctacct
     3361 tattcattat ccctgctatc tgtataactt ctttttcttt cagacctctt tgagatattg
     3421 atgtaagacc tatacgaaca ccactagtta caaatggtga ttgtgggtcg tcaggtataa
     3481 catttttatt aactgttatt cctacatatt caagagcttt ttcaaattgt tttcctgtaa
     3541 gattcttact tcttaagtca accattacta tatgattatc agtacctcca cttacaatac
     3601 taaaaccatg tgaaatcagt tcagaagcta gagtctgagc atttactaca acttgctcca
     3661 ttatcttttt aaatttatct gtacttaaat ataatagtga aaaggctttt gctgcaactg
     3721 tattaagatg tatagaacct aatgttcctg gaaaaacacc tttatcgagt ttctttgcat
     3781 gttctgcttt acaaagtaca ataccacttc ttggtccaca gaaagtcttt gtcgtagatg
     3841 aagaaacaaa atctgcataa ggtacaggac taggtattac tttcgctgcg actaatcctg
     3901 caacatgtgc catatctacc ataaagtaag ctcctacttc ttttgcaacc tttgatattc
     3961 tttcataatc tattaatctt ggatatgaac ttgcccctgc aattataagt tttggtctac
     4021 attcttttgc ctttgcttca agagcatcat aatctatacg tccagtattt gggtctaccc
     4081 cataaaattc atatttataa atgtcatgta aaaaattaac tgtgctacca tgtgtcaagt
     4141 gtccaccttg gtcaagacgc atacttaaaa cagtgtcatt tggttttaat attgatgaat
     4201 atacacaata gttagctgta gaacctgaat acggttgtac gtttacatgt tctgctccaa
     4261 atacttcttt tgcacgtttt attgctaggg tttcaagttt atctgcttct tcagaacctg
     4321 cttgaaaacg agcccctgga tagccttctt ctgttttatt tgtaaataca catccactta
     4381 attcaagtac ttctgttggt gctgtacttt cagaagctat catctcaata ttgtgttctt
     4441 gtctaaccaa ctcatcagct acaatcttgt ataactctgg gtctgaaatt tttgttgttt
     4501 ttaacataat aatcctcctc ttaattaaag aatatatttt attagtttaa tcttcattga
     4561 taaatttatt ataacggtct ataaaacgta agatattcct ccaccaggtc ctaatggtat
     4621 gccaataccc atgaatagta acaatattat tatttctatt aatgtaaata ccactgagta
     4681 tggaatcata ttcgatatga cagtacctat acctatatct ttatcatatc ttctggcaaa
     4741 ggccaaaata actggaaaat acggaaaaag tggacttatt ggatttgttg aagcatctcc
     4801 aatacgataa gctatttgag ttaatgctgg attaaagtct agtagcataa acatgggaac
     4861 aaatataggt gctaaaatcg cccattttgc agaagcactt cctacaaata aattgataaa
     4921 tccagatagc aatataaatc ctattatcaa tccatagcta ggtatccccg actctgctag
     4981 taattttgct cctgttattg ataaaattgt tcctaagtta ctaagattaa ataaattaat
     5041 aaactgggat gctataaatg ctaggactat atatccaccc atatcactca tactactacc
     5101 catcatagat actaaatctt tgtcactttt tatcttctta cttacttttc catacactaa
     5161 tcctggagtt aaaaatataa tagtgattat aggaactata cctttcatta aaggtgcatt
     5221 actagatagt atagaaccag tctctggatc tttcataaat gcattgtcac ctatacataa
     5281 ggcaactatc actactacta caactaacaa tgataataaa gcgtatttta tacccttatt
     5341 ttcaagttca ctcaattcct gtatatcctt atcatttgtt gaatcattat attttgtaaa
     5401 acgaggagct ataaattttt cagttacaaa tgctcctgca aaagttaaaa ctatcgctga
     5461 tataaaagca aaataaagat tcattgctgg attgccttgg taagatgggt ccatcatttg
     5521 agctgctggt attgtaaatc cagctaacaa tacatctgta gtactaagca taacatttgc
     5581 tgcaaatccc cctgcaacac ctgcaaaagc tgcaaacata cctacaagag gatgtcttcc
     5641 aattcctaaa aacacaagtg ctgcaagtgg tggtaatatt ataaatccta catctgctgc
     5701 tgtatgtgat aacatacctg caaatacgat tgctacagtt acaagttttt gtggaacttt
     5761 acttataccc tttttcatta acacttccat aaatccagat ttctcagcaa cacctgctcc
     5821 aagcattgta accagtacta atcctaaagg agcaaatgac tgaaagtttg taactatgct
     5881 acctaaatat gattgaatct gctctttctc tagtagattt actaccttta caacttcttt
     5941 tgtagaagga tgtatttctt ctactcctaa attagatata actgctgaca atattacaac
     6001 aattatacat agtaataaaa atatggttac tgggtctgga agtttatttc ctgctctctc
     6061 tacaacattt agtgccctca tcaataatcc actatctttg tttttttcct gtgaattaat
     6121 tttcctcaaa ctaatacccc ctcataaact ttatctccag aatgctctat cttaaatttt
     6181 attttttaat ttatccatat aatttataac agactgacat agtacatttg tacctactaa
     6241 tatagaattt ttatcagctt taaatttagg attatgccac tgatatatac aatcttgttc
     6301 tttgttgcct acccctaacc agtaaaaaaa tcctggggct ttttccatga atatagaaaa
     6361 atcttcccct cctgtagaag gtattggatc aactatagca tcttctgaat acaaatcaca
     6421 cacagactta caagctattg cgtacatctc tttactattt ataacagctg gtaaatcaaa
     6481 taaataatct agctttgcag ttgcttgata tccacgagct atctcacaaa ctatattttc
     6541 taaattttct gatattttct ttcgtacttc attactaaag cttctacatg tgcctgtcat
     6601 atctaccttt tctgaaatta cattatttgc agtccctcca tttatagtac ctattgatat
     6661 aacagctgat tctaatggac ttatgtttct gcttactatg gtttgaatac ccattataat
     6721 tgcacttgat gctactatag ggtctattgt tctgttgggt atacctccgt gacctccaaa
     6781 tccatttact tctatcttaa tagtgtctac tgctgccatt aacccaccta acttaacacc
     6841 tatttttcca caaggtatat ctggatgatt atgaagtcca aatatagcat caacttttgg
     6901 attttccatt actcctcgtt ccactaacat cttcgcacct ttattgactt cttcggctgg
     6961 ttgaaatata aactttacat ttccatgaag actatctcta atatgagaca atacatatgc
     7021 acatcctaca aggcaagatg tgtgtatatc atgcccacat gcatgcatta taccatcata
     7081 tctggattta tacttaatat caacctcttc atatattggc aatgcatcaa tatctcctct
     7141 tagtgctact gtgggaccat catacttccc ttttaataat ccaacaactc ctgtttccat
     7201 tcctaaatct aatatttcta tgtctaaact cactaataaa tcttttatag cttttgtagt
     7261 cctaaattct ttaaaactta attcggggtg catatgaata tctgtaaaaa aatctaaaat
     7321 atcttcttta tacttttctg agattaaaat aagttcatgc atactaacac ctctccaaaa
     7381 ttttattttt ttatttttta taatttaact acaaattata taagttaata atctttataa
     7441 agatattttt tataaattta attttagttt atatttatat tttactatat tattttaatt
     7501 aaatatatta tcatgttttg caatttattt aactattttg actttttaaa ttcggattat
     7561 ataaaaaatt tctttaaata aaaactatta ataagtggct ctattcctga ctttgtgttg
     7621 atttttttct acatgtaatt gctttataca ccatacagac taaaattaaa agccccatat
     7681 aacctgtata tggatataaa atgtttacta atttaccaaa tggtaaaaaa cctcctatac
     7741 aagccaaaat agctattact gttgttacca atttaaattt aggatgttca tcttcaacaa
     7801 ttctattggt tacagaccat aaaagtggaa cagctgttgt atagatacct aatattaatg
     7861 caactgaaaa acatacacct acaataggag atatattatc tgctaaataa agtgatggta
     7921 tttctttcat atacaaattt cctatatctg aaatcatagc caaatacata actataccag
     7981 cagtcataaa cactattcca cctaataagc ctccccaaaa agcatccttt ttactttcaa
     8041 tagaagctcc cattcctgtt aaaaaaacaa tcattacaat aatatttaat gtattatata
     8101 ttacaccaga aacgattgga ctttctgatg cttttgtaac ttcaatattt ttcatagctt
     8161 ctccaaccat agataaacca tcaaaatgct taaacaagct tattaatcca acagcaattg
     8221 taaatactgc tatcactggt ccaatacgtc caagtatgct tactaatttt tctaaaccca
     8281 tagacactgt aattaaagta gcaacagcca tcaatactct tcctaagtaa ggatttatac
     8341 catagtattc agttaaagtt gagcctgcac ctgaaatcat tactataaga atcccaaata
     8401 aaaatattgg actaaaccat tcgtaaaatt ttcctaaaat cttcccacaa taaaaagtgt
     8461 aaattcttgt tggttcttta agttttaatt ctctacctct agttataact tcagcaccaa
     8521 gaaatgaaaa caataccatt gatattaatg ctccaattaa acccatatac ccttgtgagg
     8581 caaaaaattg tagaatttct tgaccagtag caaacccaga accaattgct gttgctagat
     8641 aagcgcctgc aatacagata atatttttca tattcacttt gtctgacata ttaaattacc
     8701 tccattataa agttaccttc ctttaagaaa tataaactct cagtgaatta aatgaaaatt
     8761 ctgtattttt aaaacagtat ttaattcact ttttttattt tgtatatctt tttcttttta
     8821 tttatatata tttttttata taaataggta cagcttttat aaggtttaat attattaaat
     8881 atgtttaaag cgctttaatt tttatcataa ttaaagtata cttattcaga cacactcaaa
     8941 caattatcta tattgacttt ttatttatca atttcaattt ttagtgttca gtttataaac
     9001 ttttggagtc attccttttt ctatcttaaa gtttttataa aagttattta ggctattata
     9061 tccaactatt tctgcaatct catttatact ttttgtagta ttttcaagta actcaataga
     9121 tttttctatt cttatatgat ttagatatga tataaaagtt gtatttaaat tacttttaaa
     9181 aatcctagat atattagatg gattggttaa aaataatcta gcacaatcat ttaactttaa
     9241 gtcattgcta tagtttagat ttatatattc cagtatctca tttaataaat cattattggt
     9301 atattgatat ccaataattt tttctacaga cttagtaaat tctttcatat ccaatggttt
     9361 aaggagtata tcttttacat ttaatctcaa agctttttga gcatattcaa aatagttaaa
     9421 tgcagttatt actacaaagt ttattttttt agataaatga gatgtttttt ctattacttc
     9481 taacccgttg tatattggca tttgaatatc tataaaaact atgtctggct ctaaaccaat
     9541 gattttatct actgcttcat caccagatgt tgctttacca acaattttta ttggcatatc
     9601 tttcatttct ataaaatgtg ttattatttt tagaacagct tcttcatcat ctattattag
     9661 tgctcttctc atgagaatac ctccctttta gcttaataat ataaaaaagt aagatatata
     9721 aaacaacata aaaacatcta atatatttct taacatttat aaaacttatt aatttttcac
     9781 aagttctata catactcctt ataattttca ataggaattt taatcattat gtaagttcct
     9841 tcattatcac tcgactttat atccatatca aaattatttc cataagcaga tgtgaatttt
     9901 gtatatatta aagacaaacc atgaccatta ttactacgaa tacctgcctt tattttgtct
     9961 atattatttc catctaagat agttccatta ttatgaatct ctataatagc atactgagaa
    10021 tctaatcttg caattatttc aattcgcttt actgtgtcaa tatctctaaa tccatgtgta
    10081 aatgcattct ctacaatagg ttgaataaag ttaaaaggca cagaaaggtt atataacttc
    10141 tctggaataa gatattttac tttaagtgca tctccataac gtaatttctg taaattcaaa
    10201 taatttttaa tgtataatat ttcagattct aattctacaa atcttaaatc agacctcatt
    10261 gtatatctaa acattcttga taaatctatt attgcactat acaaatcata actaccatca
    10321 tctagcgcta tgcttgccat tgaatttaat gtattaaata aaaagtgatg atttattctt
    10381 aaattagtaa ccatatcttg agctactttt aaattcattt caagttgctc tccataatga
    10441 tatgttcttg ctatagtctt ctctttttct tgtaaatcct ttcttatatc attaaaagag
    10501 caaaaattta ttatattttt agatatttgt ttcaagaggc gttttatact tctagcagct
    10561 ccctctggaa tatcatataa attattatgc tctgtatcta aattcaaatc agataatagt
    10621 acataccctc ctcttataac acctatgcta ttacttttgt acagtattgg tatattatat
    10681 atagttatcc catattcaca tacaaattga ttgctacttt tttgattcat acaatcacaa
    10741 ttttgaggaa atttcatagg attacatttt tcaaaacaaa aaagtggaaa ataaggattt
    10801 tttaaaacta tattccatga atcatcatat ataattaatg gaattttaaa cgggtctata
    10861 aattgaccac gaattgattc tactgctgca tacaaattta atgtactttc actataattt
    10921 atcaaggtct tctttaaata attattagga ttaatatcta taacttcact ctctcctaca
    10981 tcatctacat tatttgaaac aataagttct tttatttctc tatcacctgt atttatagtt
    11041 tcatgtgtgc taccagattc tatataaaaa atcatacctt gataaaatgg cttccaaact
    11101 ccatttataa tatacaaacc ctctccctct aaaatatata tcatctgctc ttgaccataa
    11161 tgaacatgat taaaatgttt cttaccagga atagttactg caatgtatat attcatagct
    11221 tgttttggat tattttcatc ataagtatgt ttccattcaa tatatcccca ctcagtattt
    11281 tgaattttca taaaagattc ctttcctgta aattatttat gttcatattg tgaaaaatta
    11341 gtaaaaaaaa tgtgtacata ctctttcata tgatagctta ctatatcaat attcattagt
    11401 ttgtacacat tttta
//
LOCUS       DAMKMI010000126        11254 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMKMI010000126 DAMKMI010000000
VERSION     DAMKMI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.131"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            207..1382
                     /locus_tag="PC429_002983"
     CDS             207..1382
                     /locus_tag="PC429_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3848469.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            complement(1555..2952)
                     /gene="asnS"
                     /locus_tag="PC429_002984"
     CDS             complement(1555..2952)
                     /gene="asnS"
                     /locus_tag="PC429_002984"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF3848470.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     misc_binding    complement(3029..3262)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3431..3625)
                     /locus_tag="PC429_002985"
     CDS             complement(3431..3625)
                     /locus_tag="PC429_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF3848471.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     gene            complement(3898..5571)
                     /locus_tag="PC429_002986"
     CDS             complement(3898..5571)
                     /locus_tag="PC429_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF3848472.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            complement(5581..8979)
                     /locus_tag="PC429_002987"
     CDS             complement(5581..8979)
                     /locus_tag="PC429_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF3848473.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            complement(9146..10342)
                     /locus_tag="PC429_002988"
     CDS             complement(9146..10342)
                     /locus_tag="PC429_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF3848474.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDIKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            complement(10363..11184)
                     /locus_tag="PC429_002989"
     CDS             complement(10363..11184)
                     /locus_tag="PC429_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF3848475.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
BASE COUNT         3585 a         2060 c         1308 g         4301 t
ORIGIN      
        1 gtagtaaact atctgtaaat cttaattata ataaagtatt aatcaataaa gttactcgac
       61 attatctaat aaattttatc tatcacaatt ttagattgtt acacacaata tattttgata
      121 taattaaatc ataaacgaag tattccaaat atctaatgat agaaaggaat acttattttt
      181 atgctttatt tttaatggag gcttatatga acttagaaaa aataaaaaaa ttttttggat
      241 ttcctctaga caaaaaacac aaagacaatg ttgagaaaga actctgcaat tatctttatc
      301 atacgggtaa aatcatttgc ataataatag taatatctca aataattatg atgatatcaa
      361 tctcagctag agaaggaggc ccttttgtca caccaagacg ccaatggtat ttttatatgt
      421 atgtaacact attttgtgta tcttctgtct ttatgtttgt attttcttac ctttggaata
      481 aaaaaaagga ttgctgtacg ttttatataa atttatggat aatttatgtg acttttttct
      541 gcctatgggg ttgtgtaatt actctacttg accagttagg tggaaatgac ctaagtgtat
      601 ttagttatat tatgttagca gctgcagctt tctcagtatt aaaatttgga gaatcagtaa
      661 tagtttttgg aggatgcttt ttgtttctta atttattctt acctgggatt caaactatac
      721 caggcaatga gtttagtaat attgtcaaca cattttttat aacatccctt gccatttttg
      781 tttctacaat gctatataga aatcgtgtat ttacttacta tgataaactt ttaattagtc
      841 aacaatatga agaaatatct catataaata atttacttaa cgacaaagtt atgacagatg
      901 accttactaa aatgaacaac cgtagatacc ttgaagatat tctacaaaaa aaattaatca
      961 gccatgcaaa tcaagaacct ataactggaa tgatgataga tatagattat tttaaacaat
     1021 ttaatgataa ttatggacat caaaagggag actattgtct acaaaatatt gccaaagtaa
     1081 ttttagattt tacaagttct gaaaatgaac atgctatacg atatggtggt gaggaatttt
     1141 ttatatgtat ttttaactgt aatgaggcag atgctaaact taaatcagaa tgtttacgaa
     1201 aaattataga gtcctgcaat tttgagcgtg atgatgttcc tttaaaatgt gtaactgtta
     1261 gtattggagt ttatacaaag aaaattggaa agacaataac attaaatgag tttataagta
     1321 attgtgataa ggctctatat actgcaaaag aacagggacg taattgtgtt gcattttatt
     1381 aaaaggttac atactattac ataaacctat aagtattata tcgcaaaata aaagtaatca
     1441 aattatatat ttatagtaca ataaaaataa cttgcatatc tctaaaatag ataaatatgc
     1501 aagtcatttt agtgtttata cttaaagtta tataagtttt attatatata attattaaaa
     1561 ttcagcattt ttaggagttc ttgggaaagg tataacgtct cttatgttag acatacctgt
     1621 catatacata attattcttt caaatccaag accaaatcca gagtgagtag cagtaccaaa
     1681 ctttctaagt tctaaatacc accaataatc ttcttctctt aatcccattt cattaattct
     1741 atctaacaat acatcttctc tttcttctct ttgagaacct cctacgattt ctccaactcc
     1801 aggaactagt aaatccattg ctctaacagt tttaccatct tcattcattc tcatatagaa
     1861 agcttttata tcttttggat aatctgtaac aaacactgga caattatata tttgctccgt
     1921 tatatatctt tcatgctcag tttgaaggtc acatccccac tcaacaggat actcaaattc
     1981 atgcccagat tcaattaata attccactgc tttagtatac gttattctaa taaactcaga
     2041 gtttactatc ttatttaacc tttctaacaa tcctttatct ataaaactat taaaaaattc
     2101 catttcttca ggagcatttt ctaatacata gcttataaca tattttatca tatcttctgc
     2161 aagttccatg ttatcttcaa ggtctgcaaa aactatttct ggctctatca tccaaaattc
     2221 tgaagcatgt cttcctgtat aagagttttc agctctaaat gtaggtccaa aagtataaac
     2281 gtttctaaaa gaaagtgcca ttatctcggc atttaattga ccactaactg ttaaattagc
     2341 ctcttttcca aagaaatctt gagtatagtc tattttgcca tcttcagttt ttggaatatc
     2401 atttaaatcc atagttgtta ttctaaacat ctctcctgca ccttcacagt cactacctgt
     2461 tatgattgga gaatgtgcat atacaaagtt tctttcttgg aagaatttgt gtattgcata
     2521 ggcagctatt gatcttactc taaatacagc tgaaaaagta ttacttcttg gtcttaaatg
     2581 tgctatcgtt cttaaatatt caagcgtatg tctcttcttt tgtagtggat atgaactatc
     2641 tgattcccct tctacaacta tgctttttgc atgtatttcg actggttgtt tagccccttc
     2701 tgtagatact aactctccct ctacaagtat agatgagctt attgctaatt ttcctacctc
     2761 tttaaaattt tctactttct catcaaatac tatctgcata tttttaaaga agcttccatc
     2821 atttagctct ataaatccaa aatttttaga tactctagat gttcttatcc atccagctac
     2881 ttttacagtc ttccctatgt actgttcttt ttctctgtac aattctttta ctgttataaa
     2941 ttccttttgc attttaaatc ctcctagtat aattgttatt aatttctttt aaatataatt
     3001 tcaataaaaa aagcccttca tccctatata aagggacgaa agactattac tctcgcggta
     3061 ccaccctagt tgtcataatg acctcttagt taagcaatgc ttaaagccta taacggtgcc
     3121 ttccgtctaa gtctacttac taaataagct ttcgattaga aactccaaga tgttcttcaa
     3181 tataaacctc gtattgagct ttcaccatcc tcaactcgct agaactacat tttatatcta
     3241 ctcttctctt cacagtattt atcttttatt atttctgcta attaacaaaa ttataccaat
     3301 ttataaattt ttagtcaata aatttatata attgatactg attgttattt aaaatataat
     3361 aattctacct tacataatga taataaatac ttatcttcat tacttcttta ttacttcaat
     3421 ttccaaaatt ttataattta cttccattgt caacccagtc ttttgcttct tcgacaccct
     3481 taattgaagg tattgaaact ttactatgag gttttaattt ttcaacatca gcccatgctg
     3541 ctgtcgactc atttccaact aaaagtgaac ttttaagtct tttattatct ttacctttta
     3601 atgctcttgc ctttttactt tccaaaacta tcacctctat tatttatttt gtaatagttt
     3661 tagcaatttt acaaataaaa ttcataagtc taaattataa ttatttttaa attaacatca
     3721 tatcttttca atacttaatt tatacctatt tatatacaaa atatttactt tgtgtattca
     3781 aaatataaaa ctgtttttta attttcatat ttatttttta tgctattttt ctactctatc
     3841 taaagtattt gatacaatta aactaataat caattataat tttacatagg ttcttatcta
     3901 tagagtattt gctatctgtt gaatcgtatt ttttaaattt aaaatcccat atccctgcga
     3961 tacatttgga tatgtatata tctcaagttt tgtagcaccc aatattaaat atgtttttaa
     4021 aacttgtgta aacaatgata atctcggtac attatcctgt ttttctaagt attccatcaa
     4081 taaggctaaa acacctgtga ctatagaact gctcactcct gttcctgttc ctgtattata
     4141 tgttccattt ttatatgtgg atattatatc tacacctgaa gctactatat ctggcttaat
     4201 tcctcttcct ctaattggtc ctttcgaaga accaatccac atactatcag tcttattgtt
     4261 aaatgtacca actgtaatta catcatcact agcagcatac atagttatag tagctactga
     4321 atcagggtct aaaaaccttg tgtctggagc tatcaaattt ttatttggca agtatatgtc
     4381 atattctcca tttattataa gttctgaagt caatcttaaa atccatactc caggttttat
     4441 atctcttaat cttatctcca aattttcttt tccagaggta atatatggat aaataaatct
     4501 cattgcataa gtagtatttt ctaagttaaa ttttcctcta tatatataaa agtctggaga
     4561 ataccttata tcatgactaa cttcacctga aggagatatt atctgtgctc ccactttatc
     4621 tggaccattc gtatttagtg ttatatcaag agcatagtca tctccatctt gtattattac
     4681 atcttgaact tctcccacac tgctaaatct gccagaataa tgaatatctg tattcccttg
     4741 atttcctgca ccactcacta ctaccacacc agcactactc aatatattaa aagtatctag
     4801 tattgaagta gttgctacag cacttgactt aactccaata gtcaaattta ttatcagtgg
     4861 tttattttct gttcttgcta tgttagtaac atacgttata gctgctaaaa aatctgaaac
     4921 actgtaattt atccttccag cataataagt atctgtatat gattttaatt taactactat
     4981 taagtctgat tctgtagtaa tacctctgta ctgagaattt atccttccat ttccaactaa
     5041 tataccagaa actaaagttc cagtacctat attatcttga ctcaaactcc catcatttct
     5101 atttatggct atatttaact cacttcttgt aaattcactt ccaaaaataa aaccttctgg
     5161 aggtggattt gtatttgctt cttggtccca taaatataaa actttactag ttccatcatc
     5221 atttataaaa tcaggatgca aataatctat cccagaatct ataactgcta ataaaattcc
     5281 tctccctgtt atatcattat atggattttc ataaatataa tcagtttcag ctgctgttgt
     5341 aatggtttct ccattattta cattattagt tatttcaatt agactgctca ttggttcaga
     5401 ttcttcccac caagcaactt gtattatatt atttaaaata gtttcatcaa aatctaaagg
     5461 tacatatatt actgcaagct gactatttaa aaccatatac ctatttattc cattttcttg
     5521 tagggcagat tctatatcac cttgataaat tatacaataa gatttttcca taaatccctc
     5581 ctatcttaaa acatcaaaca ttcctctaac atccaagagc ccatatccag aatttgtatt
     5641 tggatatact gtgtttgaat cttttctagc accagcttgc ataaaagttt ttattttttg
     5701 cacatatgct tggtttggat atctaccgtc aacaaaagta tattgaaaat acattgcagc
     5761 agcaccagct gcatgtgcac ttgctgctgc tgtaccagtt atagttgcat acgtgttccc
     5821 aggataagca gctattatat taacacctgg tgctactata tctggtttta acctgtcttc
     5881 aatagttggc cctcttgatg aactttgcca taaactacca ttaatagtat tgtaggctcc
     5941 tatggttatt aaatcatctt gaatcgcagg ataatttata gtgtaaaaag ggtctacctc
     6001 tctaaatcta gttccacttt ttaaaagttc tctatttgga aggtataagt tatatcttcc
     6061 tgttataata taaactccta ctaatcgaat cttccataca cctctttttg cattctttaa
     6121 agtaacatta gtaaattgct gtccagaaaa agttgttggg tatatatatg ttatagaata
     6181 ttcagtcccc tctaaatcaa ataaacctgt tactttatta taatttgaaa ttccaacact
     6241 tttactttct tcaccagtgg gagatactat tattacatca gctttatctg gtctatttaa
     6301 ccaaagttct aatgataatt cttcctcatc ttcatttaac tctaattcaa cttcaacaga
     6361 acctccaaca tatggtatta ttccagatgt atgtgtctgt gtattgccct catttcctgc
     6421 tcctgccgtt atacataacc ctctagtaaa gaacgctttt tcactattac tcctattggt
     6481 taaacctgct aggctactgg tacctaaaga catatttata actaatggtc ttccaagttc
     6541 aaaagctttt ttatatgcat actgagatgc agcaaacagc attgcactat tataaaaatc
     6601 atctatcttg ccgagtttta ttattattag ctcagaatct tctgctatac ctgcatattc
     6661 actatttaca tttccaagcc ctgcacatat cccactcaac atagttcctt gaccaacttc
     6721 atcctgagat agactagggt cattttctgc gatagctcga tttatatctt ctcgtgtata
     6781 ttctgtccca atataaaaac catcaggagg agttccttct tttgtttggt cccataaata
     6841 tactattttt gaagtgccat ctggataaat aaaatctggg tgtagatagt ctattcctgt
     6901 atctgcaatt gatattagag tccctcttcc tgttatatta atattaggat tatttttaaa
     6961 gaaatttacg cccatttctt ctgttgcaac tacacctcca aaagtacctc gattaatttc
     7021 tccaagtaat gtcatctttg tagtcgatac agttcttatg attgaaggaa gttgcaaaac
     7081 tcgttgtatg ctattatagt ctgtattatt tatagatagt acacctaatg aatcacttat
     7141 cttaaaaaac ctttgactca tacctaaccc agctagttct tcttcaaatt caggagtatg
     7201 gactatatct attcctgata gtattcctga ttgtctactt gcatttttac ttatataaat
     7261 ttcattctct aagtcattat gtatttgcat attataacca tctataaact tataactatt
     7321 ttcctcatct ttaaatgact tattaatact tctatatcca atatcctctg tttctaaatc
     7381 ttgatttata ttagatagag tgtacaaatc tatatctgat aaatttaaaa aaccaaatcc
     7441 tgatgaatta tttggataag attgattact taatctcctt gctcccttta gaagtaatgc
     7501 ccttaatttc tgtgaataca aaaataaatc atttccattt acaattcccc attccataaa
     7561 caatgaacaa acccctgtaa catgaggtgt agccatgcta gttccagtta atgctccact
     7621 tgtacctcct ggtaaaaaag atactatatc ttcacctgga gctaataaat caggtttaaa
     7681 aacacctaat tgtgtatctc cttcaccaga aaatatagat actatatctg ttcttgaatt
     7741 aaaactacct actgttataa ctctacttgc tgttccagga actgttacag taagctcttg
     7801 agtaggaatc agaaatctag tatttctatt tagaccttca gaagttggca aatatatatt
     7861 tacattacca gttactatat ctattggttc aaatacaatt ttccataacc caggagttat
     7921 ctgtgtattt gaacttaatt gtagagtaac tcttcttgtg agagaatacg gtgctatagg
     7981 gtaaaaataa ccagttattc tagtttcacc caaagtattc cttatctcac ctgaagttag
     8041 agagattgct tgtgtttgat tatttgatgg gtttaccaaa tgtacactaa aatcatcaac
     8101 aaaatctggc catatattta tgtttaatat acgctctcct tccccaacta taaactcaac
     8161 ttcttctgta atattatttt gaagcctaat ccttttatga cctcctttat ctgcattatt
     8221 tccagctgct acaactatat tgtttttcca aaataaacac atatcatcta tatattgttc
     8281 aaataaagat gttcctctat gtgaaccttc attacttcca taacttatat ttaatgtaac
     8341 aggcattctt aattctaatg ccctatctaa aataaattta attgccctca taaactctgt
     8401 acttctcgaa aaaatatcgg tttgtatatt tccaactcta accactatta ttctggcatc
     8461 acttgcaata gttgcacaaa tacctgcaac atgtgtccca tgtaagctag ttgttgaaat
     8521 aggtatatac atacttccat ctattgcatt atttatatct tcattagtat ataatgttcc
     8581 ttctctaaaa ccttctggtg gatttccttg aatagattgg tcccaataat ataatatctt
     8641 agaccttcca tcactatctc taaacacagg tagagtataa tctattcccg aatcaattat
     8701 accaattata gttccttttc cagttaaacc agttctattt ttaaatcctg ttattcctgt
     8761 acttgaaaaa ctttgcacat cctgagtctg taatataaaa ggcttttcta taaattcaat
     8821 ttctggataa gttaaaagta tatttacatc ttcttcatta cttgatgtta ttattgcata
     8881 tgatgaatta agtatctcta ctgatacacc taattcttct tctaacctca atatatctcc
     8941 attgtatttt acaattaatt cataatttat tataatcacc tcgacttcta aaattattat
     9001 taatgtatat gttttgtgtg agtctaaaat gtattagact atataatttt ttaagtttca
     9061 gttatacaat ttataatagg tatattaaaa aaataaataa tgatggtaca attttgaata
     9121 aatcatacca tcatttatat ttactttatc ttgaaaccat taaatttttc aatgctttct
     9181 caagaccttt tacagtcaat ggaaacatat taaattcact ttgtatcatt ctttgagttt
     9241 gataccaata attaattgaa tcccatccat ctctaagttc tggattcatc catacaattt
     9301 tactgtactt tctcttaaac cttttaagcc actctatacc aggtgtgtaa ttatatggac
     9361 cacgatagtt accaccaata tgaagcaact cagaaggcga catagaagca tctccaacaa
     9421 ttataacttt atagttttta tctatatttt ttaatatcca ttcagtatta atagatttac
     9481 ttaaccaaca ttcaggtgtt gtaaataatc tatcataaat acagttatgg aaataatata
     9541 tctttatatc tttaaaatga tttgatttac ttacagactg aaataatgta ttacataaag
     9601 tgctataccc tctcattgaa ccacctgaat ccataagcaa taaaagcttc actgtatttt
     9661 ttcttggctt ctcaaaaaat aactttaaat atcctgcatt attacaagtc tcttcaatag
     9721 tcttatccaa atcaagctct gtctttggag catctattct agtagaaaat tgacgaagtc
     9781 tctttagtgc aacttgaaat tgacgcatat ttaaaacttc atcatctcta aaatctctat
     9841 attttctttc tcctgcaact tcaagaacag ctctaccata agtagaattt ccacctatac
     9901 gtataccagt ttgccctttt cctgaatgtc ctaactctga agtgcctcct gtgcctatcc
     9961 aataattacc accattatga ggagaatctt gttcttttat tcgttcctcc aatttagctc
    10021 ttaatttatc aagatttata tttggatttt gttctaatct ttcaaactct tctaaatcta
    10081 tatctggttt tttaagccaa ttcataatct cttctggaat ttcttcttgg tgttctatac
    10141 ctttaaaata ctccaaaaac actgcatcaa acttatcaaa atcactttca ctttttatta
    10201 aaatcatcct gcaaagatag taaaaatttg aaaagtttga atagcacagt cctttatcca
    10261 atgcttcaat taaagtcatc cattcattta aagatacatt taatcctctt gacctcaata
    10321 aataaaaaaa ttcaataaac ataattatcc agctcctatt aactataagt gtctttgcat
    10381 agattcaata tcctcattat tcttaagtag tattcctgca aaaggaactt ctttttctat
    10441 tttttcaata ggcattccac tcaaagttaa tgcttgtatc cagtctatta actctgatgt
    10501 actaggcttt ttttgtatgt cctttaaact tctaatccaa taaaaagtag tcattacttg
    10561 ttccaatagg tgttcttcta ctttatcaaa atgtacttta actatttctt ccatcatgtc
    10621 tctatcagga aattctatat aatgaaatat gcatcttctt aaaaaagcat ctggaagttc
    10681 tttttccgca tttgaagtta taataactat tggtctttgt ttagctctta cagtttcttt
    10741 tgtctcattt atgtaaaact ccattttatc caattcccaa agcaagtcat ttggaaattc
    10801 taaatccgcc ttatctattt catctattaa aagaattact tgttgatttg agctaaaggc
    10861 ctctcctagt ttaccatatt ttatgtactt gctaatatca tctacacctt ctcctccaaa
    10921 ttggctatca taaagtcttt gaacagtatc atatacataa agcccttctt gtgcttttgt
    10981 agttgatttt atattccaaa ttactaaatc tttttttaat tcatttgaaa ttgcttgtgc
    11041 tagcatagtt ttacctgttc caggttcgcc cttaatcaaa agtggctttt caagagcaat
    11101 agcaacatta actgatgcca tcaattctgg tgatactaca taatcactac ttcctctaaa
    11161 gttagatatc tcttttttta tcataaatcc tactcctttt aaacagtatt ttctaaatgt
    11221 atatagactt tattactatt tttgatataa aaat
//
LOCUS       DAMKMI010000127        11191 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMKMI010000127 DAMKMI010000000
VERSION     DAMKMI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.133"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            140..1621
                     /locus_tag="PC429_002990"
     CDS             140..1621
                     /locus_tag="PC429_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF3848476.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVYDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            1654..5091
                     /locus_tag="PC429_002991"
     CDS             1654..5091
                     /locus_tag="PC429_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF3848477.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLITYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVELGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYRR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            5109..5855
                     /locus_tag="PC429_002992"
     CDS             5109..5855
                     /locus_tag="PC429_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF3848478.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(5804..6112)
                     /locus_tag="PC429_002993"
     CDS             complement(5804..6112)
                     /locus_tag="PC429_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF3848479.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            6915..11048
                     /locus_tag="PC429_002994"
     CDS             6915..11048
                     /locus_tag="PC429_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF3848480.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISPPIGST
                     NLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDADNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFSLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWNGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPEETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNTST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEVT
                     STTPDSNLNNNISRVQVEIKQFCCNDCGCKDCCCNCCCNDCGCKNCCCNCCKNDFWC"
BASE COUNT         4522 a         1330 c         1770 g         3569 t
ORIGIN      
        1 tcttttagat gataatgata tttatttaaa tactctttat taacaattta taaaacttaa
       61 ttgtaaaaaa tacttattaa tagtaaaata aagtgaatca attatattat aattgctaaa
      121 gagagtgagg tgtagctatt tgaaaaaaaa tagattaata gcaaagaata tatcaatagc
      181 atttatagta ctattctttt ttagtgtatt tacattcttt tatgtaggta atattaatag
      241 agtgttagaa tatgaaacaa atgacataat aacagttaca attgctggat ggattatact
      301 ttcattttta tttttaggta ttataattta catattatat tcaaaagcaa atagtcaaaa
      361 aactatagaa aaagtagcat atacggattt tgtaacaggt tattccaact ggagaaagtt
      421 tgaattagat gttacaaatt tattaaagaa aacatcacaa aataataaat atgcaatggt
      481 catattcgat attgataaat ttaaagcaat taatgatata tatggccata aaaagggaaa
      541 tttaatatta aaagatattg cagatacttt aaatgaattg actgatatca atgaaacttt
      601 tgctcgtgta agtgctgata atttcaatat attattaaca tataacaaaa aagaggatat
      661 aataaatatt atcaaaaaga ttatggcaaa taatgaactt gtaaacttat cttttggaat
      721 ttatgaaatc aaagataagg atttatctgt aagtgtatat agtgatagag cttcacttgc
      781 aaaatcatca ataaaaaata atagtgatgt aaattttgca ttttttaatg acaaattaag
      841 agagaaatta ttatttgaag ataagataga aaaagaaatg gaatatgctc ttgaaagtgg
      901 gcaattcgta atgtatttac aaccaaaata caatattaaa ttagataaat tttgtggaag
      961 tgaagcccta gtcagatggc aatatacaga aaaagaggtc atatatccag gtgattttat
     1021 tcctatattt gaaaaaaatg gatttattag aaagattgat atgtacatat tagaacaagc
     1081 ttgtaaagaa atccggtctt tatttgataa aggaatttca cctttaccta tatctgtaaa
     1141 cttctcaaga gttgattttt ttaaaaaaga ttttattgaa aacatagtaa atatatgtga
     1201 tagatataaa ataccatata gtttaataga aattgaaatt actgaatcta gtatgtttgg
     1261 agatactgat actctattta atgttagtag aaacctacag gatattggct ttatagttgc
     1321 aatggatgat tttggctctg gatattcttc tgtaaatatg cttaaaaata taccattaaa
     1381 tgtaataaaa ttagatagag gattttttgt ttatgataaa gatgttgata agagtcaaat
     1441 tgttataaaa agcatagttt ctctcattaa gcaattagga ataagggttg ttgctgaagg
     1501 aattgaaaca agaagtcaaa tagaaatgtt aaaaaaagca aattgtgata ttgttcaagg
     1561 atattatttt tcaaaaccat taccaattaa agaatttgaa aaattagtct ataaaatata
     1621 gtcagcgagt cttaaatggg aggtggtgat taagtgataa gagcaaaatc gttaaaaaat
     1681 gtactgagta ttgttttaat atttttattt acaataattt ctttttctga ttttgcattt
     1741 aaaactaatg tccaggaaga agaaaatagt aaaattaatt taagagaaat atccctacaa
     1801 agttccaatg ctataaaaat aaaattagat agttctctat cctttattaa aagtgtggca
     1861 aatatggttt cttattttaa tactatagat aactcaaaaa tagttccatt attagctaat
     1921 atatcaaaag aatctgactt tcaaagaatc tctattgtat ctcctgatgg aataagtcat
     1981 accagtgatg gcaaaattat caatgtgtca aagtatcagt attttaagaa agctattaaa
     2041 ggagaaagta atatatctga agttgtgaaa tctccaattg atggaaaaga tacttttgtt
     2101 atagctactc ctgtatttaa taagaataac aaaataattg gtgttttata cggttcttac
     2161 tacaatgata aattagctga atttatagat ataacaagtt ttaatagtga agggtacata
     2221 gatatatttg aatctagtgg tcaatttgtt ttaaaatctt cacatacaaa caaactgatt
     2281 aagtcaagta gtaacatata tgatatattt aaaaaagctt cttttgatga tggttatagc
     2341 tataaaaaaa tatatgaaga tacaaaaaat aacaagagtg gctttacctc atatgaatac
     2401 aaaggagaaa atcgttatgc tacttataca cccattggtg taaatgactg gtatgtaatt
     2461 tctgtgatac cacaatatac tgtaagaaga aacattgata aaataaatca attagcattt
     2521 tctcttattt tcaaggtatt cttattgttt attatgctta taacatatat tatttataaa
     2581 aacaataaat ctcagaaaga tatactaaaa gctaacaaag aaatctcatc tattacaaat
     2641 agcattcctg gaggagtaca aaagtgtacc ctgggtgaaa catgcaatat taatttttta
     2701 agtgatggat ttatagagtt gtttggttat acaagagaag aaataaaaac attgtttaat
     2761 aatgattttt atagtttaat acataaagat gatgttgaaa gaataaaaaa agaaattttt
     2821 aaacaacttt acaaaaataa aactgttgaa ttagaatata ggattataac aaaaaatggt
     2881 aatacagtat gggttttaga taaaggggag cttataacag aacctgatgg aaaagaatat
     2941 ttttgttgtg taactgtaga tattactaga acaaaacaat tagaacaaga gatggaacaa
     3001 aaaaatctag aacttgaaat gatatcagaa aatgttttag gtggtataat tataacagaa
     3061 tttaatgatg attttactat aaaatacgct aactctggtt atctttcaat gattggatat
     3121 acacgtgaac aattagaaaa tgaacttaaa tacaaagctt gtaatcttgt tttttatgaa
     3181 gatagaaaga aggctcttga aagtatacaa catcaattaa acacaaaagg aatagctgta
     3241 tatcaacata gaataagaag aagagatggt aatattattt gggtttctat aaaaggaagg
     3301 cttattgttg atgatgatgg tattgaaaaa ggtatttgga ttctaaatga tataacagat
     3361 ttaaaaaatg cagaacaaaa acttaaaata aatgaagaaa gatttagaat tgcattaaag
     3421 caaacatcaa acactatatt tgagtatgat ataaaaacaa agtcactttt aaatactagt
     3481 aaagagcttg ataaatataa tatttataaa attactaaaa atgttccata ttctctagta
     3541 gaattaggag ttattcataa agattttagt gatgatttta taaatatgta tgaaaatgta
     3601 ataaatggag caaaaaaagc aagttgtgta gttaagatga aacttccaga tgaaaattat
     3661 atatggaata aagttacctt gactacgatt tttgatgaag atggtaatcc tgtaagagca
     3721 attggtatac aggaagatat aagcaaacaa aaggaagcag agataagata caatcaagaa
     3781 ggtaagtatc attcagctat attgtcggaa gcaatgataa cttatgaaat aaatgtatca
     3841 aaggatactt ttatcaaagg aaatgaaagt tggcaaaaac catttggaat taagcctgac
     3901 aataactatt ctcaaatggt aaaaactata gcaaatagta ttgtacataa agacaataaa
     3961 gaaacatttt taaatacatt tttatgtgaa aacttattgg atgcatataa aaataatgtg
     4021 actgaaataa aattagaata tcgaagaatg aatcaagaaa ataaaatgat ttgggtcatt
     4081 tgtacaatgc acttaataaa agattctatt acgaacgata taaaagcact tgcttatgtt
     4141 aaagatattg acaagcaaaa aaatgaagaa attagattaa aattaaaagc tgaaagagat
     4201 actcttacag gattatataa taaaggtact acagaaaaat tgattaatga tttcttgaac
     4261 tctgaggaag caagcttagg aatgcatgca tttttaattg ttgatataga taattttaaa
     4321 tctgtgaatg ataatttagg acatgtattt ggagataaag tattatttga agtttctaaa
     4381 aacctaaaac ctatatttag aaaagatgat atagtaggta gaattggtgg agatgaattt
     4441 atagtattct taaaaaatat tcagtctttt gagaatatca aaaagaaagc agaaaaaata
     4501 tgcgatatat ttagaaattc ttatacagaa aataaaaaga catataaaat atctggaaca
     4561 gttggaattt catttgtacc agaacatgga actgtatttg aagaacttta taaaaaagca
     4621 gacatagcac tttattatgc aaagagcaaa ggaaaagata agtatgcact ttataatgat
     4681 tgtatctacc aaaatgaact tgaaaataat ataagtgata ataatataaa gttagcagat
     4741 aaatgtgaga aatccaaacc agtacttaaa gaaattcttg attctattga taattatatc
     4801 tatatagtga atccaaatac ttatgaactt ctttttgtca ataataaggt gtctcgtttg
     4861 gatttagaga taaaattagg agaaacctgt tataaatcac tattaaaatc tgatagacca
     4921 tgtaataatt gccctcttaa cggattagaa gaaaataagc attcaaaatt caaaatagag
     4981 acaaatacat ttggtaaaaa tatcagtata gcaagttgga ttaattgcat agatggagaa
     5041 tacaactgtc ttatagagtg tatagattct tctttataca aaaataaata aaaaaggact
     5101 atgaagatat gttaaatagt ataactttga tacttggtat aatatttaca atttactatt
     5161 tttatctcaa aatagttttt ggaggaattt catttagtga gattttctta gttgtaggaa
     5221 ttgtactaat tatctatcaa atatttaaga aaaaaattaa agaaaagaag gttttttata
     5281 gggtgttaaa aataataatc agcatttttt taatagtatt tgtagttact gagagtctaa
     5341 ttatttttta tcccaaaaat agtttagaat caaaaagtga ttatctctta attttaggag
     5401 cttctgtaaa aaagactaca ccaagtacaa ccctgaaggg taggttagac actgcattga
     5461 aatacctaaa aattaatgat aattgttatg tggtagtatc tggtggtaaa ggaagtggag
     5521 aaaaaataac tgaggcaaaa gctatgaaag attatcttat aaaaaatggt atagataaaa
     5581 ataggataat agaagaggat aaatccacga atacttatga gaattttaaa tattcaaaat
     5641 tgaagataga agaacatagt caaagaaaac taagcgattt aaaagtaaaa atagtaacaa
     5701 cagattttca tgttctcaga agtaaaatat tagcatatag aaatggatat aaaaatacgt
     5761 ctttttatgc tagtaaatca aaactttcat ttgtaccaac ttactataca agagaatttt
     5821 ttgcattatg gaaaacaatc atttttgata gataataaat aaaaagacta gatataaact
     5881 catatctagt cttttctatt cccttaattc taatccattt tccaacttta cttgttgtag
     5941 aaatttcaat attgggattt acaagtaatt gcttataaga agttttatat ttttcgattc
     6001 caaaatataa ttttccttca taatccatta taaatccaaa tggtcttact cttggtttat
     6061 tatcttcaac tgtagcaaaa tagaatgttg agttttcttt taaaaaattc attatttttt
     6121 catgattaaa tcctctttgt atcttatact aatataatga atataaataa aatattcatt
     6181 atacttaata taaatcgata taaaaacaat tacaatatta tattgattat tcgaaattat
     6241 atataaaatt cagaaaaaat tttagtttac ataacaaaaa taaagtcaac taaaattatt
     6301 gattttagat taaagtaccg gattaagcat ttaagtagat tttaagcttg atttgattgg
     6361 aaaggtacaa actatcaccg aaaaaaatta aacccattta aaaagcaata taaaagcttg
     6421 ttatttttga gaaaaataga ccctctaaac tcaattagat taaaaataaa atacagaaaa
     6481 ttatatctct aaatatctta aataattaag tttaaatttt acgatatttt gtttgattat
     6541 ttataatata aaagcatact cttaatcttc aaaatttaaa aagagtaaaa aataacaaaa
     6601 aaatcactga catttcactg acaaattgta attttgttat tataaataca ttgaaaatac
     6661 ttgattattg aaaaatggca taaaataata cattttatgc catttactat tatttgatag
     6721 tttttaactt atgacataag atatttttta acacatacaa taaaatataa aaaaattgat
     6781 attgcaaata aaaaaaatac atattagatg ttatagaaaa aaagcgcact tataggctta
     6841 aaatgtcaca taaaagagtt ttaaagaata atatattatg aacacaaatt ttgtaatcac
     6901 aaaggagtga taatatggca acagtatcag gacaaatagt ttttgataga aatagaagtg
     6961 ctaccattga ttctggagac tcaggaatag ccaatatacc agttgtatta caagatatag
     7021 caactggagt tagacttgtg gttctcacag atgcatcagg aaattatgtt tttactaatg
     7081 ttccaaatgg taactataga attgtagaag cttttggaac tacaggagga gtaccaacac
     7141 cagggaattt tgcaacagcg atagtaggtc cagttccaat tgcagaaatg ccacctatta
     7201 gtcctcctat tggttcaaca aacttagatg gtacaacacc taacacacta ttaataacag
     7261 tagcaggttc agatataaca aatcaaaata tattaaatgg gcctgtgatt tatacaccta
     7321 tagagaatat attggattct tgcgtatctg tatctaatat aaaccttatt acagatgcag
     7381 ataacggaac attcggtttt tttccaccag gtacaccaag taatacagga cctgaaatag
     7441 aaccttaccc aaatgtgacg ccagatttta cgtatgttgt accagaccca actaagttta
     7501 cacctttaga tggagaatat acagtacaaa atattatgac aaatgcaatg agtaatgtta
     7561 taggagcttg gtggcgtatt gctgaccata ctacaggaaa tgaaacagga agaatgatgg
     7621 tggtaaatgg ttttaatcct ggagctgtat tttttaaatc aacagtacaa gttacaccta
     7681 acactaatta tttatttagc acttggattt taaacctttt taaggtaaca ggatatccac
     7741 caccacagct aggtgtaaga atatttgacc aaaataataa tatactatat caagcaacat
     7801 taggagctca aattccagtt aatacaaacg caccagaatg gaaagaagta ggtactgtga
     7861 ttaattctca aaataataat caacttacag tagaattttt tagtgaagga gaaagcgcca
     7921 ttggaaatga ttatgctata gatgatgttt ctttaagaga aatagctgtt ccaactttta
     7981 ctccaataaa aacaagtaac acagaaactg ctaatgtagg tgatataatc acatatacag
     8041 ttacattaga taatacttgt gatagccctt taactaatgt attttttagg gatttaattc
     8101 caaatggttt agagtttata cctggaactg taacggttga tggagtatct gtaccaggag
     8161 tagaccctaa tacaggtttc tcacttccag atattggagg aggaacaggg gttgaagtaa
     8221 cttttgatgt tgtggttgaa agtattccta acccaaatcc tactaataat atagctaata
     8281 tagattactc ctatacacct gtagaaggcg gtataccaaa tgatttttca gtagattcta
     8341 atccagttcc tgttgaagta atatcagcag atattgaagt tacaaaatta tctgaaccta
     8401 ctatagtgaa tccaggagag gaattaatat acactataaa agtagtaaat aatggaccat
     8461 tcccatctga aaatgtggtt ttaacagatg atgtaccagc ttctattata aatccagagt
     8521 attcattaga tggaggagta acttttcaac catggactgg aagtttaaat atagggacat
     8581 tagaagtagg agagacaaga gttattataa ttaggggtat tgtaaatcca tcaacagtag
     8641 gaattataac aaatacagct gtagtatctt caactacagc agacccaaac cttaataata
     8701 atacatctac aatagagaca gaagtgaatt tattagcaga tattttagta atgaaaacag
     8761 cagaacctaa ttcagctgtt ccaggtactt tattgagata tactattcaa gtagaaaact
     8821 taggaccagc aaatgctgaa aatgtaatat taaatgatga tattccagct tctattataa
     8881 atccagaata ttcattggat ggaggagcaa gttttcaacc atggaatgga agtcttaata
     8941 taggaacttt aaactctgga atttcattaa cagttttaat tcaaggaaca gttagtttaa
     9001 attcttcaga atatatagta aatacagcaa cagtttcttc aactacacct gacccagatt
     9061 taagcaacaa tatatctacc attattacac cagttaaccc acaagcaggt atttctatta
     9121 taaaagtagc tgacgaggat gtagctgttc caggagaaga atttgtatat acaattgaaa
     9181 tttttaatga aggaccatct aatgctacga atgtagtttt aacagatgat ataccagatg
     9241 ttattttaaa tccagaatat tcattggatg gaggagcaac tttccaacca tggaatggaa
     9301 gtcttaatat aggtacagtt gcaccagggc aattaataag aattatcata aagggattag
     9361 taagttcaac tgctacagga gatattacta atacagcaga agtaagtgca gatgtaccag
     9421 aaccagttac ggattcatca acagtgacaa ctcctatagt tccatcagca gatatagaag
     9481 taataaaaac tagcaatatg gatacagcag ttccagaaga aactttcagt tatactataa
     9541 cagttacaaa tttaggacca tctgctgcac aaagtatcgt tttgacagat gatataccag
     9601 atgttatttt aaatccagag tattcattgg atggaggagt aactttccaa ccatggaatg
     9661 gaaatcttag tataggcact ttggatgctg gagaaataag aagtattata ataagaggaa
     9721 cagtgagcca gacagcagta ggtacaatta taaatacagc aacaataagt tcacctacac
     9781 ctgacccaaa tccagataat aatacttcaa cagatgaaac agatataagt ccattagcag
     9841 atatttctgt gataaaaggc aacgaaccag ttgctatacc aggaggaaga tttatttatg
     9901 gaattgaaat tgcaaatgca ggaccatctt ttgcagagaa cgtaacttta acagataata
     9961 taccagcttc tattttaaat ccagagtact caatagataa tggagtgact ttccaaccat
    10021 ggaatggaag ccttaatata ggtactttag atgctggaga gataagaaat atcataataa
    10081 ggggaacagt aagtcaaaca gcaataggta caattataaa tacagcaaca gtaagttcaa
    10141 ctacacctga cccaaatcta aataacaata cttcaacatc agaagcagag gtaagttcat
    10201 cagcagatat ttctgtagta aaacgaagca atcagactgt tgttgtacca ggagatgtac
    10261 tagactatac aattgaggtg agaaatgcag gaccgtctac tgctcagaac gtaactttaa
    10321 cagataatat accagcttct attttaagtc cagaatattc aatagataat ggagtgactt
    10381 ttcaaccatg gaatggaagt cttagtatag gcactttaga tgctggagag ataagaaata
    10441 tcataataag gggaatagta agtcaaacag caataggtac aattataaat acagcaacag
    10501 taagttcaac tacacctgac ccaaatccag ataataatac ttcaacatca gaaatagata
    10561 taagttcatc agcagatatt tctgtaataa aattagcaaa taaaacagaa gcatgtgtag
    10621 gagagcaaat agattttgta atagtagtat caaatgcagg accagaaaat gctcaaaatg
    10681 ttactttaat agacaatgtt tcagataagc ttaagaaagc agtattttca cttgatagag
    10741 gtgtaagttt ccaaccatgg actggaagtc ttaatatagg tacactacca gctggaactt
    10801 taagagtgat tatattaaga ggaattataa aatcaacatg tcttgataga ttaacaaata
    10861 ttgcagaagt tacttcaaca acaccagatt caaaccttaa caataatata tcaagagtgc
    10921 aagtagaaat aaaacaattt tgttgtaatg attgtggttg taaagattgt tgctgtaatt
    10981 gttgttgcaa tgattgtggt tgtaaaaatt gttgctgtaa ttgttgtaaa aatgactttt
    11041 ggtgttgatt ttataaaata attttgtata ccttaaatta aactgaaata aaaaataaat
    11101 tcacatatga gtaaaatatt ttataaatat taataaattt tctatctttg atatggacca
    11161 cattcgtaat actagttatt aaatgaagtg g
//
LOCUS       DAMKMI010000128        11122 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMKMI010000128 DAMKMI010000000
VERSION     DAMKMI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11122
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.134"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            248..694
                     /locus_tag="PC429_002995"
     CDS             248..694
                     /locus_tag="PC429_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848481.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVP"
     gene            1075..1992
                     /gene="pstC"
                     /locus_tag="PC429_002996"
     CDS             1075..1992
                     /gene="pstC"
                     /locus_tag="PC429_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF3848482.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            1994..2818
                     /gene="pstA"
                     /locus_tag="PC429_002997"
     CDS             1994..2818
                     /gene="pstA"
                     /locus_tag="PC429_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF3848483.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2837..3601
                     /gene="pstB"
                     /locus_tag="PC429_002998"
     CDS             2837..3601
                     /gene="pstB"
                     /locus_tag="PC429_002998"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF3848484.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3629..4282
                     /gene="phoU"
                     /locus_tag="PC429_002999"
     CDS             3629..4282
                     /gene="phoU"
                     /locus_tag="PC429_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF3848485.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4440..5309
                     /locus_tag="PC429_003000"
     CDS             4440..5309
                     /locus_tag="PC429_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF3848486.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5396..6832
                     /locus_tag="PC429_003001"
     CDS             5396..6832
                     /locus_tag="PC429_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF3848487.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6880..7707
                     /locus_tag="PC429_003002"
     CDS             6880..7707
                     /locus_tag="PC429_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3848488.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    8003..8265
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8341..11007
                     /locus_tag="PC429_003003"
     CDS             8341..11007
                     /locus_tag="PC429_003003"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF3848489.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         4392 a         1194 c         2082 g         3454 t
ORIGIN      
        1 aaataataag attaagaatt tcttaataaa tccagaagat gctttcgatt atataatatt
       61 ttatattttt atattattaa ttatatgtgt tgcaatcgct gcacctactt tttctggtga
      121 gtatcagaca caatcagata gcatccttcg ttgtacgaaa catggacata ttcgtctagc
      181 gtttactaaa atattagctc tattttctat atttataata atgtttgtta tatgtatttc
      241 tatccatttg gcaatttctg atttagcttt tggtactgaa tgtttgaaaa cctcatttca
      301 aatgttgttt tctgtcatta gtctagtaaa tataaattta ctacaagcac agattcttat
      361 agctattggt ggtctaattt caatacttgc aatggttagt ttaacattat ttttatctgc
      421 aaaatgtaag gattctctta cttctatgct gattgctttt tctatttgct taattcctat
      481 gggaataggt gttagttgga ttggatacct attaccacca gggggaattg gttttaagat
      541 tagtttatta tatcaaatga catcatttaa tttcttacac ttaggaggta tgagtttttg
      601 gacaccacac attatactgt ttttctcagt gatagaaata cctattttta tatttttggc
      661 tattcgtacc tattgcaaac atcaagtacc gtaacaattt gatggaaata atagtgatat
      721 aaactagagg atacaataat tatatatgta gtataaaagt taaatttagt taacaaatct
      781 taatagcaga tataggtaat aaattataca taaactgtat tattatctat atctgctatt
      841 ttaatataga gaaacaacag tatattgtaa agtaaatgtc aaatatagtt ttaaaagtaa
      901 aattaatgtt aaaaagtaaa tataatttta cacttgctta acttaatcct aatatagatt
      961 taacttaaaa aaagtagaat aaaagtatag gaaatttgat gaagacgaat tataaaaaat
     1021 taaattttga atacagaacg aaaatcgata agtatatttt aatgggaggg tattatgata
     1081 gtaaaatcta gattagtagg tttagaaaaa aatgacaata atggcaataa gacaaaatac
     1141 gtaattgaaa aagtagcaaa aaatattttt ctacttagtg ctttagtagc agttgcaagt
     1201 ttacttttga taataggatt cgtattttat aaaggattaa gaccatttat atttgaagga
     1261 tattcattta tagacttttt aactggtgca gattgggttc cttctgccaa taaatttgga
     1321 atatcatcta tgatagtagc atcaatattt gcaacacttg gagcacttgt tataggagta
     1381 cctgttggta tattaacctc tgtatttata gcagaagtag cacctaaaag attagcaaaa
     1441 ataatgtcac cagcagttga acttttagca ggtatacctt cagtattata tggtgtattt
     1501 ggtcttgcag tgatagttcc aactatacaa ggtatattta atcttccaaa aggtcaaagt
     1561 ttgttagcaa tagtaatagt attagcagta atgatgttac caacaataat atctgtatca
     1621 gaaacagcaa taagagcagt accaaaagca tacaaagaag gttcattagc acttggtgca
     1681 tcaaaaatag aaacaatatt caaagttgta ttacctgctg caaaatctgg agtattagcc
     1741 gcagtagtgt taggagtagg aagagcttta ggtgaaacaa tggcagtaat acttgttgca
     1801 ggcaactcac cagttatgcc aactgcaata acagatagtg taagaccact tacaactaat
     1861 atagcacttg aaatgggata cgcatttggt acacatcaag aaatgctatt tgcaactgga
     1921 gtagtattgt ttacatttat attaatttta aacttagttt taaataaact ttcaaataag
     1981 gcggtaaatt aagatgagaa agttcaaaga gaatctattg aagtcattag tatatctatc
     2041 agcactattt acaatagtat cattagtagt tatagtggga tttatattta taaagggaat
     2101 tggcaacatg aattttaatt tcttatttag caattattca gcatcaggtg atggtggtat
     2161 acttccaatg ataattacaa cattatatac agtagtagtt tctatagttg tagcaactcc
     2221 tataggaata ttggctgcta tatatttaca agaatatgct aaaaaaggaa aagcagttac
     2281 agctataaga tttgcaactg aaagtttatc agggatacca tctatagtat atggattatt
     2341 tggaggaatt ttctttgtag ttacattaaa aatgggatat tccatagttg caggttcact
     2401 tacagttgca ataataatat taccagtaat aattcgtaca acagaagaag ctttaaaaac
     2461 agttcctcaa gcttatagag aatcatcatt ggcattagga gctacaaaat ttcaaacttt
     2521 atataaggta gtacttccaa gtgcaatacc aggaatttta tctggagtaa tcttatcagt
     2581 tggtcgtata attggagaat cagctgcaat attacttaca gcaggtactg tagccaagat
     2641 gccaggtgga atatttgata gtgcaagaac attaactgta cactcatact tactgactaa
     2701 agagagtgga gatatagcaa cagcagccag tataggaatt gttttaatag taattgtatt
     2761 agcactaaac atgctagcaa gatttgttgc taagaaatta aataaagcta attattaaat
     2821 ttaagaggtg tgaataatgg aattaatcga taagataaaa atgagtgtaa aagatttgga
     2881 tttattttat ggtgataagc aagcattaaa gaaaataaat atggatataa aagagaataa
     2941 ggttactgct ttaataggtc catctggatg cggaaaatca acttttataa gaacgttaaa
     3001 tagaatgaat gacttaatcg aagatgtaac aattaaagga aacatttctg ttgatggaga
     3061 ggacatatat acctcagatg atgttataaa tcttcgtact aaagtaggaa tggtatttca
     3121 aaagccaaat ccatttccta tgagtatata tgataatgtg gcatatggtc ctcgtacaca
     3181 tggactaaga gataagaagc aattagataa aattgtagaa gaaagcttaa aaggtgcagc
     3241 tatttgggat gaggttaaag acaggcttaa atcatctgca ctaggacttt ctggtggtca
     3301 acaacagcgt atatgtatag caagagcaat tgctatgaga ccagaagtta ttcttatgga
     3361 tgaacctaca tctgctttag acccaatttc aacattaaaa gtagaggaac ttatagagga
     3421 tttaaaaaaa gactacacaa tagtcatagt tacacataat atgcaacaag ctgctcgtat
     3481 atcagatgaa acagcttttt tcttaaatgg agaggttata gaatttagcg atacaaaaac
     3541 tatgtttacc actcctgtag ataaaagaac agaagactat ataacaggta gatttggtta
     3601 aaatatataa ctataaagga gttagacaat ggtcaataat agtttagata taagtattga
     3661 tactttaaaa gaacaaacag tgcttatgat ggaaaaatgt gaagaagcaa tagaaaagtc
     3721 tgtgacttgt atgatagaga aggatttaga gggtgcaaga aaagtagtag aagatgatga
     3781 agaaataaat aaattaagag aagaaataag agataagagt atagagctaa tagtattaaa
     3841 gcaaccaatg gcaagggact taagatttat ttattcatta ggtaatatat ctatagagct
     3901 ggaaagaata ggagattatg cagcaaatat agctagagaa atgttgaaga taggtggaga
     3961 ggcttatata aaagagctaa tagatattcc taagatggca aaagaatgta agtctatgat
     4021 gtctcagact agagaagctt taatcttatt tgatgcaagt atagcttatg atgttggaac
     4081 taaagatgat attgtagatg gattgtatga acaagtacaa attgattgtt taaaaataat
     4141 gcatgaaaac tcaaatacca tagaccaagg ggtaaaactt ctacttatag gaagatacct
     4201 tgaaagaatt ggagaccata taacaaatgt atgtgagaag ataatttttg caattgaggg
     4261 taaaatggtt gagataggat aaaaaactgc tttaaagcag tttttttatt gtctaaatta
     4321 agaaagaatt gataaaaaaa tatgattaat gggtaaattt aaaaaattgt gatatactag
     4381 gaacatatta aatatgagga tgcgtatttt atataaggtg ctttttagga ggtggaactt
     4441 tgaaacttca aaagatgggt atgaggacta taaaaacagg aattagtgtc actttatgta
     4501 tgattcttgg tgactattta gtagagaata tgttttattc tctggtagca tgtataattt
     4561 ctgttcaaga tacagtaaaa ggttctttaa agacgggaat caatagagtg gaaggtacta
     4621 ttttaggtgg tataatagga ttcatatttg caatcattaa agctggagac ccatttttat
     4681 gtggtctagg agtaatgtgc tcaatatatt tatgcaatct gtttaaaata aagtcagtag
     4741 tagttgcctg tgtaaccttt ctggctatac atctaggtgt aggaaatagt gaccctgtgt
     4801 attattcttt acatagggtg ttagatacat ctgtgggtgt tttgtttgga gtacttgtta
     4861 attattatgt tgctagacca aattatctaa atagtgttat agatgagttt aaaaagatag
     4921 aagaactttc aatgttgctt attgaagata aaattcttag aaaagaaaat ttaaatgtag
     4981 agaaatttga agaagaagta gaaaaattag aagaaatata ttcaaaactt attgatgagt
     5041 tggattatag tttgagtgaa actaatatag aaaaaattga gaatgcctta aggatatgta
     5101 gagaaataca ttttcatatg cagtctatag agttacttga aaataaactt tatttgaaag
     5161 agcatacata taaatacctt aagaaaacat ataatgtgga taagctagac tggaatatag
     5221 atgaagaaaa aagccctgta ttcaactatc atctaatgaa aataatgatt gaaatagagt
     5281 tgttgagaaa gctaaataaa tcatattaat aatagtagtt aaataataaa taaaaaatag
     5341 tatctttgta aatattaaac atatataagt ttaatttatg gaggtactat tttttatgaa
     5401 tactagaaat gacaattcta aaatactaga aatatataaa atgaagcttt ttggcgaact
     5461 tgtaaaagca gtgtggaatg atgatataga ggatattgaa aacttaccta ataaaatttt
     5521 aaatgaagat ttaaaggata atttaaataa ttattttagt ggaaaatttg ataaagatac
     5581 tatggtaaat ttaattaggg taactatggg attggatgtt acagaagata cagataaaaa
     5641 tttaaaagag tcactgcatg aagctgtaaa tttagatgaa gtcaaaagac caataatatc
     5701 tgtaatatca gatgcttgca aatattgtga tgaaaataat aaggaagatg agtcatgtaa
     5761 gataagaagt aagcatatta attgtaatga ggaagatgta tgtagtgctt gtggtgattg
     5821 tgtatcaaga tgtaagttgg gtgcaatttc agataaaata gaatttatac caatgataaa
     5881 attgttaaaa gatataaaga gccctgtata tgcaatagtt gcaccagctt ttgtaggaca
     5941 gtttgggaaa gatgtaagcc ctggtaaatt aagaactgca ttaaaaaaga taggatttga
     6001 agatatgata gaagttgctt tggcagcaga tatgcttact gcaaaagaag catatgatta
     6061 ttatgagcat atgaaaaagg acgaagaagg atgctttata actagttgtt gctgtcctat
     6121 ttgggttagt ttgatacaaa atagttttcc tcagatattg gaaaatgtat caccatccgt
     6181 ttctcctatg attgcttgtg ggagagctgt aaaaatttta aatccagatg ctaaagttgt
     6241 attcataggt ccttgtatgg caaaaaagaa agaagcaatg ttagaagata taaaagatgc
     6301 agttgatttt gtgcttacat ttaaagagtt gcaagaaata tttgatgcat tagagttaaa
     6361 tccagcaata atggatgaga gtaatagagt agaggcgtcc ctttcaggta gagtttatgc
     6421 tagaacagga ggtgtaagta aagcagttaa gttatcagtt agaagaattg acaaaaatat
     6481 aaaatttgaa gaaaaggcat ttgaaggaac aaaagagtgc atggacggtc taaagaaagt
     6541 tctaaataaa gaaatagaag ctacatttat agaaggaatg gggtgtgtag gaggctgtgt
     6601 aggaggtccg aagcgtattt taagtgtgga agaaggtact aagaatgttg atgaatatag
     6661 tgaaagtacg aatatggaaa ctccatttga taatttaaat gtattacaat ttttaacttc
     6721 tttgggaatc aaaagagtag aatctttagg agaaaaagaa gaagaaaagg ttaaaaaaat
     6781 atttagtaga gatataaaaa gtaataagaa agagaataaa tataaaaaat aactttttaa
     6841 gtcttaaact tatgctttta taaaagggag atatggggga tgaaaattaa agataataag
     6901 gctgtttttc atgtatttat aaggatattt cttatagctg ttttaatttt aactggaaca
     6961 ttattggagg aaaaaattaa taatgttcca gttgaaaata taagttcaaa aggtgaaaat
     7021 acactggaac tgctttctca aagtaatact ttaggagaaa tcatatacaa agcatctcaa
     7081 gaagaaacaa aaggtccagg ggctacttct gaaaaagtag catacataac aatagatgat
     7141 ggtccatcaa aatatacaaa tgcactactt gatattttaa aagaaaatga tgtcaaagcg
     7201 acatttttca tgcttaatca gaatatgaaa aaccacaagg aagaagtaag gagagtcttg
     7261 gaagaagaaa actcaatagg ttttcatagt gtaagtcatg atatacataa gttatataaa
     7321 agcccagaag taacagttgg ggaatttaat acatgcaata atactctaca agaaataact
     7381 ggtcagagtt caaaattaat aagattacca tacggaagta aaccatatat gccagaaggt
     7441 tcatataata aacttatggc aaatgattat ttaatttggg actggaattt ggatacacag
     7501 gattggaagt cttctacaag tcagatagta agtaatatat tatattatgg aagaaaaaga
     7561 gatgaattag tagtattaat tcatgagaaa gagcaaagtt taaatgcttt aaataatatt
     7621 ataagaatct taaaagagag aggatataca atattgccaa ttacagaaaa tataacacct
     7681 aaaaatttct ggcaagaaaa cttataataa atttaaatat agtaaaagat aaagtaaatt
     7741 atttataaaa taaaacatat aatataaaaa atacgaataa gaggtatact gtaaggataa
     7801 tataaaatta atgagaaagc attaaaatta aaataaaata atcttgcaat tattgacatt
     7861 ataagacaga ataactgtaa aaacatattt taaaatataa caactatata aaagagaata
     7921 gacataatta agaaaattca gaaaaaataa aataaaaata attgacaaag agaataatat
     7981 ttgtatataa taatatgtaa tattaaagac tgagaagaag aggagtaagt aaatactact
     8041 aaaagagagg aaaattcact ggctgtaaga ttttctaagt ataagattta ctgaatgtag
     8101 ctttggagtt tctaaactga actataagta agtttagacg gttagaaccg ttaaattctt
     8161 tgagagtcaa agttgataaa ataacattaa ccaataattt aattatatgt taatgtattt
     8221 taagctttgg aattaaggtg gtaacgcgag cttttcgtcc tttttaaaga ggatgaagag
     8281 ctctttttta tttctaaaga tatttaaaaa atttatttat aaaatacagg aggcaggaca
     8341 atggaaaata cgaatttatc aaaaacttat aacccaaaag attttgaggc taggctatat
     8401 aaaaaatgga tggatgaagg gtattttaaa tctaaaccaa atccagataa aaaaccattt
     8461 acaataatga tgccaccacc aaatataaca gggcaattgc atatgggaca cgctcttgac
     8521 catacacttc aagatatatt aatcagatgg aaacgtatgg atggatatga agctttttgg
     8581 cttccaggaa ctgaccatgc ttctatagca acagaagtta aagttgttga aagaataaaa
     8641 aaacaagaag gcaagacaaa gtatgaaata ggtagagaag aatttttaaa gagagcctgg
     8701 gaatggaaag atgaatttgg aggaaaaata tctaatcagc ttaaacaact aggtgattct
     8761 tgtgattggg ataaagaaag atttactatg gatgaagggt gtaatgaagc agttatagaa
     8821 ttttttgtaa gcctatatga aaaaggtcac atatacagag gaaatagaat aataaactgg
     8881 tgcccagatt gcaaaactac tttatcagat gcagaagttg aacatgaaga acacgacgga
     8941 aacttttatc atataaagta cccactaaaa gatagtgaag actttttaga aatagctaca
     9001 actagacctg agacaatgat aggagatact ggtatagcag taaatccaga agatgataga
     9061 tataaacatt taataggaaa aacagcaata cttccattag taggaagaga acttccaata
     9121 gttgcagata gttatgttga tttagagttt ggaactggtg cagttaagat gactccagca
     9181 catgacccta atgattttga agtaggatta agacataatc ttgagcagtt aaatactatg
     9241 aatgaagatg gaactatgaa tgaagtttgt ggaaaatatg aaggtatgga tagatttgag
     9301 tgtagaaaag ctatcgtagc agacttaaaa gaacaaggtt acctaattaa gataaaagag
     9361 cataatcata atgtaggaac ttgttataga tgccatacag tagttgagcc tagattatca
     9421 gagcaatggt ttgttaagat ggaagaactt gcaaaaccag ctatagatat tcttaaaaaa
     9481 ggagaattag aatttgttcc agataaattt gataaaactt atttacaatg gctagaaaat
     9541 ataagagatt ggtgtatatc aagacaatta tggtggggtc atcaaatacc agcttattat
     9601 tgtcaagagt gtggagaaat agtagttgct agaaaaatgc cagataagtg tcctaaatgt
     9661 ggaagcactc attttaaaca agatgaagat gccttagata cttggttcag ttctgcatta
     9721 tggcctttct ctactctagg atggccaaat aagactgaag ctttagatta ttattatcca
     9781 acaagtgtac ttgttacagg atacgatata atattcttct gggtagttag aatggcattt
     9841 gcaggtatgt tctgtatgaa tgaaaagcca tttgaccatg ttttggttca tggattagta
     9901 agagattctc aaggtagaaa aatgagtaaa tctttaggaa atggtatcga cccattggag
     9961 ataatcgagc aatatggagc tgatgcccta agatttacat taacaactgg aaattcccca
    10021 ggaaatgata tgagattcta tatggaaaga gtagaatttg ctagaaactt tgcaaataag
    10081 ttgtggaatg catcaagatt tgtatttatg aatattgatg aagatataat taaaaacatg
    10141 actagagaaa gtgttaaaga ggacttaact ctagctgata aatggataat atcaagagca
    10201 aataatattg taaaagaagc aactaataac atggataagt ttgatttagg aatagcactt
    10261 caaaagatat acgattttac ttggtcagaa tactgtgact ggtatattga aatggttaaa
    10321 cctagattat atggggaaga tgcaaatgct aaatctgcag cattatatac attaacttat
    10381 gttcttgaaa agatattaaa attattacat ccatatatgc catttataac agaggaaata
    10441 tatactcatc ttcctactgt tgaaggatgt ataatagtat ctgaatggcc aaaatataat
    10501 gaagaagata acatggctga agaagaagat atgatgaact tattgatgga aggtataaga
    10561 agtataagaa atgtgagagc agaaatgaat gtaccacctt ctaagaaagc taaattgata
    10621 ataatcccaa gtgaagaaaa aatagaagct attgagcttg gtaaagacta ttttataact
    10681 ttagcatctg cttcaaatgt agagatagca aaagataaat ctaatgttcc agaagatgca
    10741 gttggagtag ttattgatgg tgttgaaata tttatacctc ttaatgaatt agttgatttt
    10801 gaaaaagaaa ttgagagatt atctaaagaa aagaaaaagt tagaaggcga aataaagaga
    10861 gttaatggaa agcttgcaaa tcaaggtttc ttagcaaaag ctcctgaaag tttaattgaa
    10921 gaagaaaaag caaaaaaaga aaaatttgaa gagatgatta aatctgttga ggaaagatta
    10981 acaaatttag aatctaaaat aaaataaata taagaattag taaaaacata caaaaagtat
    11041 atattctata ctttttgtat gtttttcttt atatatatac aaatatgatg ttataattct
    11101 ttacagatgt gttatatttt ta
//
LOCUS       DAMKMI010000129        11097 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMKMI010000129 DAMKMI010000000
VERSION     DAMKMI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.135"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(15..647)
                     /locus_tag="PC429_003004"
     CDS             complement(15..647)
                     /locus_tag="PC429_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF3848490.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(719..1072)
                     /locus_tag="PC429_003005"
     CDS             complement(719..1072)
                     /locus_tag="PC429_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF3848491.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1150..1353)
                     /locus_tag="PC429_003006"
     CDS             complement(1150..1353)
                     /locus_tag="PC429_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF3848492.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(1743..1973)
                     /locus_tag="PC429_003007"
     CDS             complement(1743..1973)
                     /locus_tag="PC429_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3848493.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1975..2382)
                     /locus_tag="PC429_003008"
     CDS             complement(1975..2382)
                     /locus_tag="PC429_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3848494.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(2742..3191)
                     /locus_tag="PC429_003009"
     CDS             complement(2742..3191)
                     /locus_tag="PC429_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848495.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3205..4131)
                     /locus_tag="PC429_003010"
     CDS             complement(3205..4131)
                     /locus_tag="PC429_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848496.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(4256..4660)
                     /locus_tag="PC429_003011"
                     /pseudo
     CDS             complement(4256..4660)
                     /locus_tag="PC429_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            complement(4810..5430)
                     /locus_tag="PC429_003012"
     CDS             complement(4810..5430)
                     /locus_tag="PC429_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF3848497.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5529..6797)
                     /locus_tag="PC429_003013"
                     /pseudo
     CDS             complement(5529..6797)
                     /locus_tag="PC429_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6889..7704
                     /locus_tag="PC429_003014"
     CDS             6889..7704
                     /locus_tag="PC429_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDF3848498.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            8376..9143
                     /locus_tag="PC429_003015"
     CDS             8376..9143
                     /locus_tag="PC429_003015"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF3848499.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9471..10148)
                     /locus_tag="PC429_003016"
     CDS             complement(9471..10148)
                     /locus_tag="PC429_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848500.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10298..10615)
                     /locus_tag="PC429_003017"
     CDS             complement(10298..10615)
                     /locus_tag="PC429_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848501.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10637..10975)
                     /locus_tag="PC429_003018"
     CDS             complement(10637..10975)
                     /locus_tag="PC429_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848502.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3659 a         1772 c         1608 g         4058 t
ORIGIN      
        1 taaattataa aatattaaac taatttatca atatttcctg attgaatatg cttaatgttt
       61 gattgaattt ttttatatgg ccaatcccac catttaattt ctaataattt tgaaattgta
      121 tcattattaa atctcttttt tataattttt gcaggtactc ctccaacaat agaatacggt
      181 ggaacgtcat tagtaactac tgctcttgtt ccaataattg caccatcacc aatatttaca
      241 cctgacataa ttatagaatc ataacctatc cacacatcat ttccaattat aatgttaccc
      301 ttattgtccc aagcgtccgt tatatggttg atatcgagtt tccattcttc ataaaagatg
      361 ggaaaagtat atgtggattt tgatttcatt gagtgatttc cactagtcat tagaaatttt
      421 gcattacatg ctattgagca aaacttacca attattaatt tatcattgtt tatagggtaa
      481 tgatacaata cattgttttt ctcaaaatct cttgggtcat tataaaagtc attatatatt
      541 gtgtaatctc caacttctat attttccctt gttattacat ttttaagata tacagtttga
      601 taatcattac ttcttggata taccttattt gaacttggaa tagtcatatt taaatctctc
      661 ctttaatata tattatgttt ttgactattc cactactaca atacatctct tcacagaatc
      721 actccttgtt aagaatcttc ataagataat ggcgtccttt tttagtgtta agtcggatat
      781 tctttcttag tccattcttt ttagcgtata actgacaaac tgtcattttc ttaccaatag
      841 tgtcaatgct atcatcaagg tcattttgta ttgctttttc cttatccctt aatgatatat
      901 catcacgttt tccctttggg ggtttatcag taggtacaag tttccaagag taaacaaatt
      961 gtggtttacc aaatgaatat acatacttat atgcgtatct tccatctttt ctctggctct
     1021 ctccagttct taaaattcta tctttattat ctcgtctttt ttcagttgac atgataagaa
     1081 gctcctctca ataaccgaat tgagcttgat acgtcatatg aatattataa catataaggc
     1141 tcaatttcat tagataaagt caagtgtatc aacgattttc tcaaattgtt ttctctttat
     1201 ttgaatgcga ttgctattca taatgaccca gccagaatca aggttttcat cagcaagttt
     1261 acgtaatttt ttctctccaa tacgaaagta ttttgacgct tcttcaatcg tcaaggtata
     1321 tttttcccaa ataggtacat cagtattgtt catagaatgt atcactcctt tctatgttcg
     1381 tagttcagaa gtagatagat ggctttggta agctccattg gtattttaac caatcccatt
     1441 cttatgggca gaaccgtgtc atacaggtgt atcattatac tgtggtacag gttacggcaa
     1501 aacaaccgtt ccaaaggtta ttcacaaatc actatatagt agctctcttg attaaggtca
     1561 tggcgtatag tatttgttgg ctatctgtat cacaaacgta tctatctact ttattcagtt
     1621 gtcaaagaac agtcggcatt gatttgtcta taaaaaatca caatagctta tgggctttta
     1681 tagacaaata attagtgatt ctccatgtac caaatagctt gatacatgga gagagggcga
     1741 gattaagaca aattaaatct caaaatagca gtgattagtt ttgttcctag cattcgcttt
     1801 gtttcttctt ctacatgaat atagggattt ccgtattcat caaataaagt tttctgtgaa
     1861 agacgaatga tatagtgttc aaaatgttta agtattccgt ttattgcatt tacatcgcca
     1921 tgtacagcaa gtaaaataat tgtaaaaggt aatacttctt tagtaggttt catttcaatg
     1981 ttcctccatg agttctttca actcttttag tgcatttgtt ttgtgataat gtatggtatt
     2041 ttctgctaaa tccatcaatg ttgctatgtc agcatttgtc atgttgagaa agaatgaaag
     2101 tagaattata tcttgttttc tttttgtaag ttttccgatt gcttgtgcca ttaatacatc
     2161 ttcaattcgt atgttgtagt cgtaagaaac catacagatg tagttagaat cataatcatc
     2221 taaaatactc aatttactta attctgcaag tgacaaagat tcgagagata ccaaataatc
     2281 atttctgcgt ttattttcgt cataaatatc tctagcatag ttacgcaaaa ctgttttaca
     2341 gaaactgtcg aattgatgtt caattgtctt ttcatcaacc ataagatttc accctctttc
     2401 catcgcaagg agttaaataa ccttgcttac tcctatcacg gtgggagggc gttaaatctt
     2461 cgataaattt caaaaaaaag agaaaaaaat acacactctg cctatagggc aaaaatgtgt
     2521 attctttaaa acgtatttaa tttatattgg ttgtaagcca ccaccatatt gatgtatgga
     2581 aatatgcatt ggctagcttt tcataaaaat acaaagatgc ataagaaatt aatagagggg
     2641 aaaaacttat tctgttatta aaaagtaggt taattctaat aaatgctatt gcggtcgaat
     2701 taaactatat acatggcaat caatcatgtt tctattcttt attatgaatt gtgacagtat
     2761 tcttcaatct taaaaccaca tttttccaat actttacttg accctctatt gtaggaatat
     2821 acattagcat gaattttgtt aaggttgtat ttttcaaagg caattttgca tatttctttt
     2881 actgcatttg ttgctatccc tttgccccaa tagttcctag caagccagta acctaattca
     2941 gcatttttat taatagaggt atcttcttca aatgttaggc tgatacttcc aatagccctt
     3001 tcttgataaa aaatagcata gctaagttgt ttaacatctg gagttgccaa acaaaaacta
     3061 acaaaatctc tagcgttttg ctccctataa gggtggggga agttgtcacg aagattcttt
     3121 gcaatttctt tatcattcgc aaactcaact aaatcggtaa tataagcata attccactct
     3181 ataagttcca tttttcttct ccttttatga aacagctaca agaaatcttt ctaagccaat
     3241 accacttatc aaacagcgtt cttttttatt tccaagcatt tgataagtcc agtcaacaaa
     3301 tccaccgtca actatttcaa cggtttcatt tccttgtttg accgataatt tgaaattgat
     3361 acctttataa taattattat caacatcatc attatgaatt gataactcta tattaggaaa
     3421 aataagtttg atagtttcaa ccattctgtc gaaaaatccg tcattatctt tgtatccgtt
     3481 tcttttgcgt agaataatag agaaacgaac attttctatt tcacttaata tatctttata
     3541 atagtttaag tgcttttcta acaacatttt ttcacaagtg taagaccctg tatctatacc
     3601 agatgaaacc atacaatata agccaaaatg tgaatataaa cctttaccta caaaattttg
     3661 tgctcttatt accctagcag tagtagccat gtggataggt atggtattat tttcttcttt
     3721 tctttttaat ttatcagcaa taattattgc caacatattt gaggggtctg caagtgtttc
     3781 tgtccctcgt agagcactta ctacattata ttgatctacg gagccaaaaa cgctacagct
     3841 acctaatgga gcagagggag aaagcataat cgtcttgata tccatattga atgcttttct
     3901 caataatttt gactccaaag catgaatttc ttctggatct ataccgctag ggatagtaaa
     3961 tctatttagt tggtatgact ttagaatatc agttgcagtt aaagtatttg cttgtctatc
     4021 aaacacttct aatagaagtg agttcaaatc tgattttgat agagctgata acttatcaac
     4081 tatattctta tccccaattt tatttgtaat tctacttaaa atatcactca tagttatcct
     4141 ccatactgtt actttcaaaa taattataga atggttacaa caactaatct aggtcatttg
     4201 accaaaaatg aaaaaacact gaatatttac tattcaatat ttttttactt tgggtttatt
     4261 tgattaaatc ttgaaaatac tgtaatgcaa ccctttcagc ttcaaccttt cggacaatag
     4321 gactttgtga taattcctct cttaactcaa gatacaggtt gatagtagcc tgcaatgcat
     4381 taacacagct tttatattca tggttagcaa ccgtcttttc taatgcttca atatattggg
     4441 gtgcgtccaa ttcaattttt ctaactccac gaggtaattt acctaagaga agctgtatta
     4501 atggggcaag tactgattgt cttaaaaaag ataaaaaatc aattgtttca aatagttctc
     4561 tacgtccaat cttagtgcaa gcgtaatgaa cccaaatcca aaatctatct tcaatccact
     4621 gtaagtctgg agttgggaag tatgcttctt ttttagtata acacctctca ctttctttta
     4681 ttagctatca atccaacata attatgcata ggagtattat ataatgaaaa aataaaaaaa
     4741 aataggtcac ttgacccgaa ataaaaaagc attgaataat tattattcaa tacttttttg
     4801 aaattatact tatgttgtag gaagattctt taatacctct cgctgattaa ttacactaaa
     4861 tcttttgttt tcataagcaa ttagatgttc ttgtacacat tcatttatag cacgattaat
     4921 gcttctaatt ggagcgttta cttcgctagc aagctgtttt ttggtaagca gatttaattc
     4981 acttctataa tagtgtaagg atatacacct cagaagtcgt tctttaacac ttaaagatga
     5041 taattcttca attaattcag catttacaac taatttacta gatatttctc gaatcagaaa
     5101 ttttgtagct tcaaaatctc cccttaacca gtttaaaaaa tcatctctat gaagtttata
     5161 tacattacaa tctgtgaaag cgacaatttc aacgggtttt ttcccatcat taaattgttc
     5221 catttctcca aaaaaactac cacttctata aagatgaata gttgagatgt ttcctttcac
     5281 atttaagatg aatgcttctg caataccgtc aatcataaga attacatatt cattttcttg
     5341 ctcagcaaaa agaatagtct ctttcttttt atacgttttt ttaacaagct tctttttaat
     5401 atcttcattc ataaaatcaa ttaaatgcat attcatccct ccttaccaaa tctttgatta
     5461 aattatatca tctttcaatt agttaggtaa gatagtaaaa atatatttac atagaaacta
     5521 ttccattatt attttccagt tgtcattttt ctgcaaagta agctcaaact gagaaagctg
     5581 ttgacaatag aactagtagg aatatatgag cgaaccattt cagtattcaa ctgttgtagg
     5641 ttttcagtca gataattctt caaacgttca attcgtttgt cctgtgaatc ttgcgatgct
     5701 ctaaccagtt tcatagatag cgttttacct tgtgccacct gcaaaaaaga ccatgcttcg
     5761 tcaaggtcaa ctatcttgaa gctagagcgg tcagtatgaa taaagtctaa tgcaaaggta
     5821 ctaataacaa tcaacatagc aacactaaga agttctattg ttgtatattc ttcaaagcta
     5881 gtgttggcat ctggcaatac gaggtcagca acttgaataa tattcagctg tttctccaag
     5941 ctgatagact gcttcacatt tccgtcacca aacagtagat gtgcaaaatc atagtctaca
     6001 aaactttcga tatggtcggc tatgctgtta ctgatagaag tatcttcttt tcgtaattca
     6061 tcaatgacaa gtaataaccc tcgctgttca ctttgggtta cattgcggat tgcttttcta
     6121 agcactggga acttatcact gtcacgactg gagataccag caaggaatgt taggatatca
     6181 atcgctaaac tttcagaatc atttttacgg ctcaaaatga cgtaagggtc aagcaatcct
     6241 ttgttttcat cattactggt taaattgaca atgtttatct catgggctat ttctggtagc
     6301 gtttctttcc atttgccgcg ttctgcttta gggtcaacta tgaccgcctg tccgccgaat
     6361 agtaccgcat agtaaacaag catattgtta ctgaataatt taccaccacc caatgaaccg
     6421 ataaacgcag acgctaacac attggtaata gaacccttta cccattgact agcaagggca
     6481 ggttttagat agacattctt tccagtgtcc agattgtagc caagatagat atcctcatgt
     6541 tcgccaaggg tttgtgtcgc tccaaaacca agtccagcca aaaaatccga agtgacatat
     6601 tgaatataat cattgatgta acgtttgctg acaggaataa attcgccatg caatcccaac
     6661 atatccccaa agggacgcac gagcttgagt gataagtcgt cgtaaaagtc tttgacttca
     6721 tcacaacgcc tttttagctc gtcaagatcc tttgtagata cacggacaac atagctaagt
     6781 ttatacatag attctttaca gttaaaagtc tgtttttaaa aaaatgtata aattataaat
     6841 caaaatatag atactatcaa tgacccacta ggtgcaaaaa gcattgatat ggttaaatta
     6901 tcagacgcta aaagctgggc tatccgaatg agtgaagatg ggtgtgctta taaatccatt
     6961 gacaattata agcgctcact gaaagcgtcg tttttcatgg caattcaaga cgactgtatt
     7021 agaaagaatc catttaattt tcagttaagt gatgttttgg aagatacaac agaaccaaaa
     7081 atcgctctaa caccagaaca ggaaatgggc ttattatcct ttatggagag cgataagact
     7141 tattgtaaat attatgatga tgttgttatc ctcttagaaa caggactacg catttctgaa
     7201 ttatgcggtt tgactttaga attggatatg aaaaataaag ccattatggt agaccatcaa
     7261 ttgttgagtg atactaagat tggctattat gtagaagttc caaagaccac acagggtaag
     7321 cgagaaattc caatgactga aagagcttat caatcatttg aacgtatcgt ggaacggcgt
     7381 aagaacgcta aagcagaacc aatcattgtt gatggttacc agaacttcct atttcttaaa
     7441 caagatggat taccaaaaca agcaactcat tatcaaagca tgttaagagc gttagtcaag
     7501 aagtataata agacccatga ggacaagcta ccaaagatta cgccacatac ctttaggcat
     7561 acactctgta caaatatggc taacaaagga atgagtccta atatattgca atatattatg
     7621 gggcataaaa acattactat gacgcttggc tattatgcac atggttcatt tagttcttcg
     7681 agagctgaaa tggaacggtt ataatcctag tagtatgact agagtttact acttatttac
     7741 tacttttgat ggcattctta tacggttaaa tgccaactta tgcaaagtat cttccaaaaa
     7801 gaaaatgccg atactccttg tagatacagg atatatcgac atttaccaac ttatgaaaag
     7861 ataatcaaat ttaatataag tttttcaaat aaaaagatac caaattcttt ttcgagaatc
     7921 tatataacat tacttaagct tggttggggt ataaacaatt gagtagctac ttttgtatgg
     7981 tgttcaactt tagtcaaaac tcttaagtaa tgcaaatgat ttagattcat taatattcaa
     8041 tctcttcata agtagtgaaa ttcgagttat ttctgcataa ttatactatg atttatagat
     8101 taattctatt agcatccaga ataatattgt ttatatgagt aaaatttaaa gaatattgtg
     8161 aaaattatag gatgttaaaa taaatttagg taaaaaagaa gtgtttgtat attatagagt
     8221 accttttcta aaatatgtgg gattgtttta agaattagaa taaaaattat ttttaaatta
     8281 gtatttttat aatgtatagt atttttaatg tataattaaa ttgtgcagaa aatagaattt
     8341 aaagtcctat agaatttctg attatggagg agaatatgaa aagaaaagtt caagtaaaaa
     8401 atattactat tggagaaggt agacctaaaa tttgtgtacc aattattggg aaaaataaaa
     8461 aagatattat aaaagaggct aaagagttaa aagatgcatg tttggatatt atagagtggc
     8521 gagttgattt ttttgagaat gtagaaaata ttaaagaagt taaggaagtt ctctatgaat
     8581 taagaagtta tatacatgat atccctttat tattcacatt tagaagtgta gtagaagggg
     8641 gagaaaaatt aatatcaaga gattattata ctactttgaa taaagaaatt tctaatacag
     8701 gtttagtaga tttaatagat gtagagttat ttatgggaga tgaggtcata gatgaagtag
     8761 ttaattttgc tcataaaaaa gaagtgaaag taattatatc taatcatgat tttaataaaa
     8821 ctccaaaaaa agaagagatt gtatctcgtt tatgtagaat gcaggaattg ggcgcagatt
     8881 tacccaaaat agctgttatg ccgcaaaatg aaaaagatgt gttagtgcta cttgaagcta
     8941 caaatgaaat gtttaaaatt tatgcagata gaccaataat aacaatgtca atgtcaggaa
     9001 tgggtgttat aagtagattg tgtggtgaaa tatttggttc tgcattgact tttggagctg
     9061 caaaaagtgt ttcagcacca gggcaaatat catttaaaga gttaaattca gtacttaatc
     9121 tattacataa gagtattaat taaattaatt atatagaaat attagacggt atttttgaag
     9181 atataaatta aatataaaaa acttttcaga tagtaaaatt taaattgggc aattatatct
     9241 gaaaagcaac tatttagtgt gtattttgaa agtgatatag aaaatcttat aaatcaacta
     9301 tggcatattt aaaatatgcc attaaaacct actagacatt agattataga tggtatacta
     9361 gcatttctat tatttataga tagtaagttt tcataataac tgttttatct aaagtagaag
     9421 atatatttgt attatgtata tttgtcatta tttttttgta attatgtata ttaatggtta
     9481 tatatgtaat ataatattat cactctattt tttgtatatt gtacattaca acatttatat
     9541 ctatcatcat caattacagt agaatgtatg ggatacttat tttcacttaa ccaatatttt
     9601 tgcatattct ttgtccaaga ctttggaaac ttcaatactt tcttgtttat tataacatcc
     9661 tcatcttcaa aaatattaac ttctctttct atttttattt ttctgttgta tatcttattg
     9721 atagattctt tgtcgagttt tatgtctcta tacatatcag ggcaatagca acaaacataa
     9781 tttctttttt ttcgacgttt aaatttcaca actttctcta tgctaatttc taattttttt
     9841 gtaccatctt ttataaattt aatatcttct atgttctcgc ttgaaaaaat ttctgtcaat
     9901 cttaaagtat ctacagtttc tctaattatt tgctttttat aagaagagaa tccatatcca
     9961 aaagaaaggt ttaatttatt agggatgtag ccatttttat atttaaacat cttaattttt
    10021 tccaattcag cattatcttt aaatttcaac ttttcatacc taaaagttac tttgctaaaa
    10081 agagtatatt ctacatcaat atattctttt gcaatcttaa atttatcgct accttcaatc
    10141 acaatcatag cctatctcca atcgttttat attaaaataa ggccatagaa catggtctaa
    10201 tagtactcat tagcactata agacaatgtt aacacaaaaa gacaaaaaaa ggttatttat
    10261 ttcaaataac cttttttttt atataaaata taaagaatta tattttattc ttttttatga
    10321 tgtctataat ctgtgatatc agatttagtt ctcttgaatt taagtctttt aactcttcat
    10381 ttatatcatt ttctaaatat gtttttccag tggaagtaat atcttttata agaacatctg
    10441 cagaaatatt taatatattt acaaatttta tgaatgtttt aagacttgga ttctttttac
    10501 cactttcgat ttgagttata taccaaggcg atactccaac catatttgca aattcttttg
    10561 aagttaaatt tgcttttttc cgttcttctt taagtctttt acctatataa ttcattattg
    10621 ttactatttc cctcacttat atttctttgt atttaatgat gcactttatt aactctagca
    10681 ttgcaatttg ttctttttca gttaatttat taatcatttc ataacattct aaaggtacag
    10741 agttatcaac gccttctgtt gtaataatat ttgcaaataa caattcaagt ggtatgtcta
    10801 aagcagtagc aattttcatt ataatattga tagatgtatt tgtctcagct ctttcaatct
    10861 gaccaatata tgttggatgt aaacctgcct tttctgctag aaactcttga ctatatccca
    10921 attttgttct atagactttt attctatctc ctattatttt tcctatattt gccaattatt
    10981 atccctccac tttagattat attcatagtt taagttgttt tatatagact atttatactt
    11041 ttttagtcta aatatagact atatatgttt ttatactgaa ataagtgcta tttattt
//
LOCUS       DAMKMI010000130        11058 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMKMI010000130 DAMKMI010000000
VERSION     DAMKMI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.136"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(53..214)
                     /locus_tag="PC429_003019"
     CDS             complement(53..214)
                     /locus_tag="PC429_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848503.1"
                     /translation="MNNFLLNVIAGVIASLIFCLICKVFLKVKSHSTRGKSKSGWEFD
                     FKIKFHKFK"
     gene            complement(555..995)
                     /locus_tag="PC429_003020"
     CDS             complement(555..995)
                     /locus_tag="PC429_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3848504.1"
                     /translation="MSNLSAFLSQNAIKVDNVKYVASNRFLDKEGKPVEWELKVLSSE
                     EDEALRRKCTKRVKVIGNNGKHTGQYTSEIDYNSYVAELCVASTVFPDLKDAELQNSY
                     GVMGEAQLLKTMLTAGEYVNYTVKVNEVNGFDTSFEDKVEEAKN"
     gene            complement(1067..1537)
                     /locus_tag="PC429_003021"
     CDS             complement(1067..1537)
                     /locus_tag="PC429_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF3848505.1"
                     /translation="MAQTINAKDTVSAKKAECFITIEGKRYNFMQAIDLEAKMEKNKS
                     EVPILGRTTKGNKTTGSTNTGSATFHYNTSIFRELLYRYKETGEDIYFDIQVTNEDPT
                     SAVGRQTVVLKDCNMDSGIITKFDADGEYLDEDMDFTFEDWELVEKFNLLAGME"
     gene            complement(1554..2864)
                     /locus_tag="PC429_003022"
     CDS             complement(1554..2864)
                     /locus_tag="PC429_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF3848506.1"
                     /translation="MALGGGTFVTQNKILPGSYINFISAKRATSSLSDRGIVAIPLEL
                     DWGIDEDVFQVTSDDFEKYSVKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CTIATAKYSGIRGNDLKVTVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITDLQDNDYI
                     TWKKDATLEASAGLVFTGGTNGEAVTGAEYQAFLDKIESYSFNALGCLATTTEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKSDADYEGVVSVENKIKDIGLVESSLIYWAAGVIAGC
                     DINKSNTNKKYDGEFDVDVNYTQIQLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     DKNDDFSSNQSVRVLDQIANDIATLFNEKYLGKVPNDKAGRISFWNDVVKHHKELENI
                     RAIEDFKTDDVSVELGNDKKTVIVSDAVKVINAMSKLYMTVSVN"
     gene            complement(2866..3042)
                     /locus_tag="PC429_003023"
     CDS             complement(2866..3042)
                     /locus_tag="PC429_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848507.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYINRKDLLNAILKENELYSFS
                     EVEEIINSFMKGVS"
     gene            complement(3035..3472)
                     /locus_tag="PC429_003024"
     CDS             complement(3035..3472)
                     /locus_tag="PC429_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848508.1"
                     /translation="MLNNIIDGISIKLDKTFGESYTIYSEDVEQGINEPCFFIVPLNP
                     SKVSYPSGRTLKKNSFDVHYFPKSNDKSFEIDEVAEMLLEELEYIEIDGDLVRGTNMN
                     FEIVDNVLHFFVDYNYFTIKNNDINKMDTVELFGGLKRGDNFE"
     gene            complement(3821..4627)
                     /locus_tag="PC429_003025"
     CDS             complement(3821..4627)
                     /locus_tag="PC429_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848509.1"
                     /translation="MAKFLDITGKKFGRLRVIKFSKEIKSGKRNRKYWLCKCDCGNFK
                     EIRTDSLTSGLVQSCGCLKKEQDKLNLTDKYQFKKKYKVQNKRLYSIWKGIISRCTDK
                     NNKRYNRYGERNIIVCDEWFCYDNFANWALSNGYSEKLTIDRINNEGNYESSNCRWVD
                     IKTQCRNRSTNILVKHEEKEITLIELSEKTGISYSCLRSRYSKGLVGNNLIEKVKIIE
                     ESRAKLSIEDVKEIRKKYSDGYTIKQLSEIYPVTYSSISNIVHRRTWKNI"
     gene            complement(4737..5027)
                     /locus_tag="PC429_003026"
     CDS             complement(4737..5027)
                     /locus_tag="PC429_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF3848510.1"
                     /translation="MARWGSVDFREFKRVCKKMEELTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYALTQWGI"
     gene            complement(5027..5374)
                     /locus_tag="PC429_003027"
     CDS             complement(5027..5374)
                     /locus_tag="PC429_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848511.1"
                     /translation="MVSKTRKAIEMLYRDKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKVAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            complement(5368..5769)
                     /locus_tag="PC429_003028"
     CDS             complement(5368..5769)
                     /locus_tag="PC429_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848512.1"
                     /translation="MENNIIDEIEKRLESFGYILKDGDKWLIGFVREKIENIIKLDCN
                     IKTMPIELKEIEVDMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTTYGKNKILTFRCLRW"
     gene            complement(5779..6012)
                     /locus_tag="PC429_003029"
     CDS             complement(5779..6012)
                     /locus_tag="PC429_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848513.1"
                     /translation="MPQVKKLNRILTIEECKIDDFLEMGYDLIDETGKAVKYGKSLSV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            complement(6031..6921)
                     /locus_tag="PC429_003030"
     CDS             complement(6031..6921)
                     /locus_tag="PC429_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF3848514.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTANVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKFFYFEES
                     FEDVFILNKKADAIQFVVEA"
     gene            complement(6933..7496)
                     /locus_tag="PC429_003031"
     CDS             complement(6933..7496)
                     /locus_tag="PC429_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF3848515.1"
                     /translation="MDWLKELLEGIKIENNKIDVVSLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLGDAF
                     KSVKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            complement(7541..7735)
                     /locus_tag="PC429_003032"
     CDS             complement(7541..7735)
                     /locus_tag="PC429_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848516.1"
                     /translation="MYILTQVIAVVCVVQIFINCIANVNVGILCNKLKEKNEANIDKV
                     SDEILKRVGEELNESLDKSL"
     gene            complement(7806..8048)
                     /locus_tag="PC429_003033"
     CDS             complement(7806..8048)
                     /locus_tag="PC429_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848517.1"
                     /translation="MIVLMNLLEKNNEYVIYSYGYEENKLDGRIKIYLDDFYNYEIIK
                     ESKDEHISKSATLKAISKLIKAAKNNDLKKEMSYQC"
     gene            complement(8014..9507)
                     /locus_tag="PC429_003034"
     CDS             complement(8014..9507)
                     /locus_tag="PC429_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF3848518.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAINSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIKYGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRDIYTSGYYHTAFNVQQGVNVGWS
                     LMSLDTNRINKIISKPWAIDGLNFSERIWGKYRPTLVNELHTKLTQSIIRGENPKNLV
                     NDFAKRFNVSKSQAKNLIMTESAFFASASRKDCFSDLEVEKYEIIATLDLRTSNICRE
                     LDGKIFDMKDYQVGITAPPFHCRCRTTTAPWFEDEEGYRAARGEDGKTYYVPSSMKYN
                     EWYEKYVKNNSKQTGAKYTKGDIEWNIRREEEAELYYDNIRNRKDDISKISKNTNWSE
                     KSIGQIKNHIFYNTHIMRDGTRRMLDSDYSMSVAWQRLINGTYEDIDILLLKHEYLES
                     IFEKKYNISNLEAHRMTEKKHDWYKELIKQKGEFEEDDCLNELIRKE"
     gene            complement(9497..10936)
                     /locus_tag="PC429_003035"
     CDS             complement(9497..10936)
                     /locus_tag="PC429_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF3848519.1"
                     /translation="MLNIYISETDLIKVQLKKESTINLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDITYFIERGNSFIQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFIPFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
BASE COUNT         3231 a         2117 c         1113 g         4597 t
ORIGIN      
        1 agaagaacta caatctattt tgcggtagag tgaagttcat aattaaatga atctatttga
       61 acttatggaa cttgatttta aaatcaaatt cccagccact tttactcttg ccacgagttg
      121 agtggctttt tacttttaga aatactttac aaattaagca aaatattaaa ctagcaataa
      181 cgccagctat tacattaagt aaaaagttat tcatacttcc cacctccttt cattaggaag
      241 taggttttat cccagtatga actccactct ataaattgta gattacatct tcttgctaaa
      301 aatattataa catataatta ttacatattt tacctattct atatttattt ttttattttg
      361 ctatcttctt cgtcccctct ttctctctct ttcagcttct ttcattgctt cctcttcatc
      421 ttctatcttt ataagtattg aggcggctgc taacgctctc tcattaactt ctaaattcat
      481 atattcactt ggcttccact ttaatttttg aatacaataa tgagtgatgc tagcatcaaa
      541 atcgccacct ctgattagtt ttttgcttct tctactttat cctcaaaaga tgtatcaaat
      601 ccattgactt cattcacttt tactgtataa ttgacatact cacctgctgt aagcattgtc
      661 tttaataact gagcttctcc cattactcca taactatttt ggagttcggc atcctttaaa
      721 tctggaaata ctgtagatgc tacacataat tcagctacat aactattgta gtcaatttca
      781 cttgtatatt gtccagtatg cttaccattg ttaccaatca cttttactct tttagtacac
      841 tttcttctta gtgcttcgtc ttcttcagat gataaaactt ttaattccca ttcaactggt
      901 ttcccttctt tatctaaaaa tctgttactc gctacatatt ttacattatc aacctttatt
      961 gcattttgac ttaaaaaagc acttaaatta ctcatattat tctaatctcc ttttatttta
     1021 atttttcata taaaaaatac acatatataa tttataaatg tgtattttac tccattcctg
     1081 ccaataaatt aaatttttct actaattccc aatcctcaaa agtgaaatcc atatcttcat
     1141 ctaaatactc accatcagca tcaaatttag taattattcc actgtccata ttacaatctt
     1201 taagtactac tgtctgtctt cctacagcag atgtagggtc ttcatttgta acttgtatgt
     1261 caaaataaat atcctcacca gtttctttat atctgtaaag taattctcta aaaatagaag
     1321 tattataatg aaatgttgca cttccagtat ttgtactccc agttgtttta tttccctttg
     1381 ttgttcttcc tagaattgga acttcacttt tatttttttc cattttagcc tctaaatcta
     1441 tagcttgcat aaaattatat cttttacctt ctatagttat aaaacattca gctttctttg
     1501 cactaactgt atctttagca tttattgttt gagccatatt atcactctcc tctctaatta
     1561 actgaaacag tcatataaag cttactcata gcatttatta ccttaacagc atcagatact
     1621 atgacagttt tcttatcatt tccaagctct acactaacat catcagtttt aaaatcttct
     1681 attgccctta tattctctaa ttctttatgg tgtttaacaa catcattcca gaaacttatt
     1741 cttcctgcct tatcattcgg aactttacct aaatactttt cattaaataa agttgcaata
     1801 tcattagcaa tttggtcaag tactctaaca ctttggttac ttgaaaaatc gtcattttta
     1861 tcatctgtaa atgatacaaa agtatttata tcctctaaca catgaacttc atctcccacc
     1921 ttgtggaata taaatttacc agtttttaaa gcttcttcaa gttgtatttg tgtataatta
     1981 acatcaacat caaactcacc atcatacttt ttattagtat tagatttatt tatatcgcat
     2041 cctgctataa ctccagccgc ccaataaatt aaactagatt ctactaatcc aatatcttta
     2101 atcttatttt ctacagacac tacaccttca taatctgcat cacttttctt atatagtact
     2161 gtttgaaact tagctcctac cttatctctc attctctttg taaattctac aaataaactt
     2221 ttaatttctg ttgttgtagc caaacatcct aaagcattaa agctatagct ttctatttta
     2281 tccaagaaag cttggtactc tgctcctgtc acagcttcgc cattagttcc accagtaaat
     2341 acaagtcctg cacttgcttc tagtgttgca tccttcttcc aagtgatata gtcattgtct
     2401 tgtaagtctg taataacctt tgctatttga gtatctacct tcttattatc taaaagtgtt
     2461 acaacatcaa acttagcatt atcatctata tttgttgtaa ctgttacttt taagtcattt
     2521 cctctgatac cactatattt tgctgtggct atagtacaac tggctttaac gcctttattt
     2581 aatttataaa aatatcccaa ccttatattt ttgaataaat ctctcaaacc tttcagcttc
     2641 tcatgagtat aatcatatcc aaaatacttc actgaatact tctcaaaatc atcactggtt
     2701 acttgaaata cgtcttcatc tatgccccaa tctaactcta aaggtattgc aacaatacct
     2761 ctatccgata atgaactggt tgccctctta gctgagataa aatttatata gctacctggt
     2821 aatattttat tctgtgttac aaatgttcct ccacctaacg ccaaattaac tcactccttt
     2881 cataaagcta tttattattt cctctacctc tgagaaggaa tataactcat tttcttttaa
     2941 aattgcattt aataagtctt ttctatttat atacttctta gaattaacta tctgctcctt
     3001 agtaaacttg taatcggttc ctttacttaa tgtcttactc aaaattatca cctctcttca
     3061 aaccaccgaa taactctact gtatccatct tattgatatc attatttttt atagtaaagt
     3121 agttataatc aacaaagaaa tgaagtacat tatctacaat ttcaaagttc atatttgtac
     3181 ctctgactaa atctccatca atttctatat actctaattc ctccagtagc atctcagcta
     3241 cctcatctat ttcaaatgat ttatcattac tttttggaaa ataatgtaca tcaaaagagt
     3301 tcttttttaa tgtcctgcca cttggatagg atactttgct tggatttaaa ggaacaataa
     3361 aaaaacaagg ttcatttata ccttgctcca catcttcact ataaattgta taactctctc
     3421 caaatgtttt atctaattta atagatattc catcaattat attattaagc atcaaatact
     3481 cctttaagca atattaatag ttttttctct ataatcttat caacttggct ttgtagttcc
     3541 atctctgaaa ttgttaagaa atgttgtcct ttaacccaac cttttccatc tttagttcta
     3601 tggccatatt caacatatgc attttgttat cctaaaggct ttttatcctc tagctcttat
     3661 agtttcctat aagttcggcg tacatcatca acaaaataaa ctttatttag ttgcccagca
     3721 ctcttggaga gattatattt attcactctc tacgctctac ggaaacctat agcctattcg
     3781 caatctatag gttttcctcg gtattggcat atataattaa ttaaatattt ttccatgttc
     3841 ttctatggac tatatttgat atagatgaat atgttacagg atatatttca cttagttgtt
     3901 ttattgtata tccatcagaa tacttttttc taatttcttt tacatcctca atagataact
     3961 tagctctgct ttcttcaata atcttaactt tttctataag attatttcct actaatccct
     4021 tagaatatct acttcttaag caagaatatg aaatacctgt tttttcagaa agctctatca
     4081 atgtaatttc tttttcttca tgctttacta atatatttgt tgacctattt ctacattgtg
     4141 tctttatatc cacccatcta caattgcttg attcataatt tccttcatta tttattctat
     4201 caattgtaag tttctctgaa tatccattac tcaatgccca atttgcaaaa ttatcatagc
     4261 aaaaccattc atcacacaca attatattcc tttcaccata tctattgtat cttttattat
     4321 ttttatctgt acatctagat attataccct tccatatact gtaaagtctt ttattttgaa
     4381 ccttatattt ctttttaaat tggtatttgt ctgttaaatt taatttatct tgttcttttt
     4441 ttaaacatcc acaagattgt actaagccac tagttaaaga gtcagttctt atttctttga
     4501 aatttccaca atcacactta cataaccaat attttctatt tcttttacca ctttttatct
     4561 ctttagaaaa tttaatgact cttagccttc caaatttttt tcctgtaata tctaaaaatt
     4621 ttgccataaa atcacctctt ttatacatta taaatcaaga gttattctat gtccatattc
     4681 tacctacttt ataatgtaac actattacac ttagcgttta ccgattttgc tgggttttat
     4741 atgccccatt gtgttaaggc atattcagtc ggattaacaa cctctataat ataattattt
     4801 ccttgtttat acacaggaag cgacctagca taagccactc cattccatcc ttgtcgtaag
     4861 aatcctgtat caactggtgt tcttctaatt actttcccaa gtaatcgtgc tgctaattct
     4921 cttgctgcat ccttgcaaaa cttatctaaa tcaatctttg taagctcctc catcttttta
     4981 caaactcttt taaactctct aaaatcaaca ctgccccatc tagccattat gctttatcct
     5041 taaataactc aagtattatt tcttgatgat ttggatatat agctgattct ccacttctta
     5101 catactcttt atcatttata ataagttttg aacctgcttt aatttctata tctggagata
     5161 taaagagttt aatagtttgc tctagcttag ctactttccc ttctgtagca gaaactatat
     5221 ttttatatga aagtttacat ggttgatttt ctaatacaat cacttcttta ttgttagttc
     5281 gttttgttac agggtcttta attggctgat actcaactat agtacattta tctctatata
     5341 acatttctat tgcttttcta gttttactta ccatcttaag cacctaaagg ttaatatctt
     5401 attcttgcca taagtagtaa gataagctat taagctatca aagcgttgtt ctggtgtttg
     5461 agaaccactt cctatagcaa aatctacctt tgtatcacct tctgatatag acttttctac
     5521 agcttcaaag ttaatgcttt ctatatctaa ttgacccata tttttcttgg taaataagaa
     5581 ctctccaact atcatatcaa cttcaatttc tttcaattca attggcatag tttttatatt
     5641 acaatctagt ttaataatat tttctatttt ttctcttaca aaacctatta accacttatc
     5701 tccatctttt aatatatatc caaaactttc aagtcttttt tctatttcat caattatatt
     5761 attttccata attttcacct actttttagt aagtttattt ttctctttaa gctgcttatt
     5821 ttcttcttct aaagactcaa cttttgacct taaaatatta ttttcagcta ttaaatcttt
     5881 tacacttaat gacttgccat actttactgc cttaccagtt tcatctatca aatcatatcc
     5941 catctctaag aaatcatcta ttttacattc ttctatggtt agtattctat ttagtttttt
     6001 tacttgtggc actatatatc actccttttt ctatgcttca acaacaaatt gtattgcatc
     6061 agctttttta tttaatataa atacatcctc aaaactttct tcaaagtaga agaattttcc
     6121 ctctgtaact gctgttggtt cgtctaactt agagaactga taagaaacag gtgtaattat
     6181 tgcacttggg tgaactaagg acataaagat ttgtttagct cctgctccta ctttccatcc
     6241 agttgtaaaa tcatatgcag ttttcattag attagatggt actttaatta ttttaactgt
     6301 gtcaatatca gttgtttgac gattaagaga agttcctgca tcctttatat ttactgttct
     6361 ttgtatctct tttgcatttt tgataagtgt atttactact ggagtaacat acaatattct
     6421 tccattttca ggtactctag cttctgtcat tttttccatt aacttatcaa atacttctaa
     6481 tacgtttgct gttgtaagaa cagttgtatc tgctgtgtta cctaatgcgg tccaatcagc
     6541 atatatttta gatatacagt aagcatccat ctctggaaac ttttgttcct cattatatac
     6601 ttttgttata ttgcctattg aagccacata attagtttgg tttatatctg ctggatgaac
     6661 caatgtagac catttccttt gattagttaa taccttaggt tcccaagcat tatcatagtt
     6721 tctttgagct actgctattg tatctctgtt tgaatctact cttccagttg tagatatagt
     6781 tggtatttct attgttttag aaccagtcca tctatatctt ccattatttg gtgttgcata
     6841 caaatccccg aagtttaaag tataaggata tgcttgtgct aaaacatttg aatattcttt
     6901 tgcataattt agtgctgcca ttttatttcc tcctatttat tattattttc atgaggtctt
     6961 accccagtaa aattaaaacc aaaatcattt atcttaggct cttgccctgg tgttatagta
     7021 tctattttag gttcttcacc ttctagtgtt gcattaaaca aataatcttt atcctgtttc
     7081 aaaggattta tttgctcttc aaaagctttt tgtctgtctt tactatttct tagtgcttct
     7141 atatctaaat gagcttttaa tgctatttca tctctacatt taacagattt aaaagcatca
     7201 cctaaccagt aattaaaatc tttttcttca atttcttttt tgtaagtttc ttccaaagtt
     7261 ttcttatcag tttcataagt tgtttttaga ttctctacat cttcttttgt catacctcct
     7321 tcaaactttt taatagtttc attagctgta ttaagctgtg tttcaagatt tgtataatct
     7381 tcttgagtaa ttgtagtctc ttttattttc ttttctatag atttttgaag agaaactaca
     7441 tcaattttgt tattttctat ttttattcct tctagcaatt cttttaacca atccatcttt
     7501 taaatatctc ctttcatttt ttacacaata aaaaaagtct ttaaagactt ttgtctagtg
     7561 attcatttaa ttcttctcct actctcttta gaatttcatc agaaacttta tctatattag
     7621 cttcattttt ttcttttaat ttattgcaaa gaatacctac attgacatta gcaatacaat
     7681 taataaagat ttgtactaca caaactacag ctataacttg agttaatata tacattaaaa
     7741 tcacctactt ttttaaactt ttacataata aaagcaccta ctaatttatt gttcagtaag
     7801 tgcttctaac attgataact catttctttt ttcaaatcgt tatttttagc agcttttata
     7861 agtttagaaa tagcttttaa cgttgctgat ttacttatat gttcatcttt tgactctttt
     7921 attatttcat aattataaaa atcatctaaa tatattttta ttcttccatc aagcttattt
     7981 tcttcatatc cataactata tataacatat tcattattct tttctaataa gttcattaag
     8041 acaatcatct tcttcaaact ctcctttctg tttaattaat tctttatacc aatcatgctt
     8101 tttctcagtc attctatggg cttctaagtt acttatatta tactttttct caaatatact
     8161 ttcaaggtat tcatgtttta ataagagaat atcaatatct tcgtatgtac catttataag
     8221 tctttgccaa gcaactgaca tactatagtc agaatccaac atacgtctag ttccatctct
     8281 cattatatga gtattgtaga aaatatgatt tttaatttga cctatacttt tttctgacca
     8341 atttgtattc tttgatattt tggaaatatc atcttttcta tttctaatat tatcgtaata
     8401 tagttctgct tcttcttctc ttcttatatt ccactcgata tcacctttag tatattttgc
     8461 accagtttgt ttactattat ttttaacata cttctcatac cactcattat acttcatact
     8521 agatggtaca taatatgttt ttccatcttc tcctcttgct gctctatagc cttcttcatc
     8581 ctcgaaccaa ggagctgttg ttgtcctaca acgacaatga aatggtggag ctgttattcc
     8641 aacttgataa tctttcatat caaatatttt tccatctaac tctctgcata tatttgaagt
     8701 tcttaaatct aatgtagcaa taatctcata tttctctact tctaaatcac taaaacaatc
     8761 ttttctactt gctgatgcaa agaaagctga ttcagtcatt atcaaattct tagcttgtga
     8821 tttagataca ttaaatctct tagcaaagtc atttactaaa ttttttggat tttcacctct
     8881 aataattgat tgagttagct tagtatgtag ttcatttact aaagtaggtc tatacttacc
     8941 ccaaattctt tcactaaagt ttaatccatc tattgcccat ggtttagaga taattttatt
     9001 tattctatta gtatcaagac tcattaaact ccaaccaacg tttactcctt gttgaacatt
     9061 aaaagctgta tgatagtatc cacttgtata aatatctctc attagtttat caataccatc
     9121 aagttcattt ccatataaaa cttctacttg ttgctgtatt tgtaacttta aagcttcaag
     9181 ccttgttata tgaactcttg cactagcatt ttctaactct ttcatccact tttgatttat
     9241 agcattttct ttaccatatt taatatattc ttcaacactc catttaaact cttctagttc
     9301 tcttgtattt agtagtttct tagcttctaa taaagatatt ccttcatttt tggcaaatct
     9361 gttgtaccat gctaatatat ctttttctat actatttata gctagtttat attgcttttc
     9421 taattcaaga taatatttta cacttttgtt attttgagct tcttctaatt gttcaaatct
     9481 ctttctccaa taatctttat gtttcatcta taacaccatc ttgattatta ggaattaaat
     9541 catcatactc tttttgagta tcttcctgtt ttttaagtct ctcaagttcg tcattaacat
     9601 cctcgaccca aggatggtta gaaacaatag tttcatctga tacaattcca gttgatttag
     9661 ctgccatatc tatcttttca gcttcattta ttatcataga gtgattaaaa gtaatttgaa
     9721 ctgttttata atcatagctc ttactaccac ttatctttaa atactcacac acaaaccata
     9781 aaagctctct aattgctttt ttaaacttct tttcagtctt agaacatttt aagtcaagta
     9841 gtgaatataa aaatttaagt gctacacccg atttgtcacc tgtgttttga gattctggat
     9901 taactccttg accaaagata attatattct tttccaatct atcaagaagc tcctttttag
     9961 cttcaactgg tatatttatc tctagtttat caactccacc tccaccatct actttaattg
    10021 atttatagta tcttatatta tctataaact cttgtagact tgttcctgga tattctttta
    10081 atacataaat aacctcttgt atttcatcta agttatctgc tagtgtagaa atattattgt
    10141 catatatatc tattaatgat ttatagaaag ttaaatctga gacacacttt tcattatttt
    10201 taaaaggtat aaatggaact ttaccccatc cctgttcttt gttatttatt ctaaaatgac
    10261 cttcttgtat atcagtcatt tttccatatt catcatataa aaattcttga ataaaactat
    10321 tacctctttc aataaagtaa gttatgtcat tttctgtgta gtactcaact ctttttattt
    10381 tatttccatc tatatcttca atataataaa acctaataaa tgcaactaat tccctctgtc
    10441 ttttactatc ccaaatagga attgcttctt cagctggaat tattacatat ttaaactcac
    10501 cttttctatt aatatatgga tgtaaccatt caaccccttt attactagca ttgagatata
    10561 gttctgttat tgtatcgtca aactcttctc ctagtaagtc atttaaaagc ttagtgagat
    10621 tatcatcatc tgcattaaat actatgggat ttccgacact atagcctacc ttttggtcaa
    10681 ctaaaagctt atggtagttg ttaattgctt tattattaac tttagtaaaa tcatcaacct
    10741 tagctccatc taagagataa tatcttctct tattgtttac atcagtatta ccataatagt
    10801 attcttctcc ttgtttatat ttttctggtc tatgctttaa gatgtagtgt tctatgactt
    10861 ttactaggtt aatggtgctc tcttttttta actgaacttt tattaaatct gtttcactta
    10921 tataaatatt taacacctcc tttactttaa gaagcttatt ccattatttt taagcttatt
    10981 atctatagaa tatctaagcg cagccattgc atcatccata aactcaactg gttcatcaag
    11041 atataatcca gttctttc
//
LOCUS       DAMKMI010000131        10910 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMKMI010000131 DAMKMI010000000
VERSION     DAMKMI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.137"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(30..1088)
                     /locus_tag="PC429_003036"
     CDS             complement(30..1088)
                     /locus_tag="PC429_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF3848520.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1155..1526
                     /locus_tag="PC429_003037"
     CDS             1155..1526
                     /locus_tag="PC429_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF3848521.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1563..2237
                     /locus_tag="PC429_003038"
     CDS             1563..2237
                     /locus_tag="PC429_003038"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF3848522.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2486..3325
                     /locus_tag="PC429_003039"
     CDS             2486..3325
                     /locus_tag="PC429_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF3848523.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3569..3665
                     /locus_tag="PC429_003040"
     tRNA            3569..3665
                     /locus_tag="PC429_003040"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3603..3605,aa:Sec,seq:tca)
     gene            3820..4527
                     /locus_tag="PC429_003041"
     CDS             3820..4527
                     /locus_tag="PC429_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF3848524.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4669..5505)
                     /locus_tag="PC429_003042"
     CDS             complement(4669..5505)
                     /locus_tag="PC429_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3848525.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5693..6598
                     /locus_tag="PC429_003043"
     CDS             5693..6598
                     /locus_tag="PC429_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF3848526.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6607..7791
                     /locus_tag="PC429_003044"
     CDS             6607..7791
                     /locus_tag="PC429_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848527.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7906..9207
                     /locus_tag="PC429_003045"
     CDS             7906..9207
                     /locus_tag="PC429_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF3848528.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9161..10204
                     /gene="holA"
                     /locus_tag="PC429_003046"
     CDS             9161..10204
                     /gene="holA"
                     /locus_tag="PC429_003046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF3848529.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10391..10657)
                     /gene="rpsT"
                     /locus_tag="PC429_003047"
     CDS             complement(10391..10657)
                     /gene="rpsT"
                     /locus_tag="PC429_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF3848530.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         4274 a         1124 c         1682 g         3830 t
ORIGIN      
        1 tgtataatag atataattat tatttaaact tattgtttat ttaatttttc aatagaaaaa
       61 gatttatctg taagtttaaa attatctttt atacccttag ttaagtatac tttcttatcc
      121 ttagttatga atatagcatc tacattatca attttattaa ttaaattcat acctttttca
      181 agaccaagac caaatgtaga agtggataag gcatcacaat taatagattt atcagagatt
      241 attgtaacac tacttaaatt gttttcaaaa ggataccctg taaatggatt taacatatga
      301 tgatatattt ttccgtcttt ttcgataaat ctttcataaa ttccagaagt aacaacagac
      361 ttatttgtag tttctatgtt tcctatggat tcaccatttt tactagttgg gtcttgaata
      421 cctattttaa aaggtttatc atttttacca ttttctaaag taaatatatt tcctcctaag
      481 tttactaaac ctttttttac atcctctgat tttaaatagg ctacaatttt atcagcagca
      541 tatcctttag ctatggcacc taaatcaatt ttcatatttt ctttggttaa ttttatagat
      601 ttattttttt catccaatat gatgttctta taatcaataa gaggcagaag tttttgtatc
      661 ttagaatcac ttggaacttt agctttatct gttccaatag cccataaatt tgataatgga
      721 ccaattgtta tgtcaaaata tccatctgat aatttggaat attgtatagc ttcttttacg
      781 acgaaaaatg tatcatcaga aactctgaca aattttttac cagcattatc atttatttta
      841 gaaacatcac ttcctggtat atgagtgctc attttgttgt caatatatct taaaattgaa
      901 tcacattcat ttaagatttt atcacttttg gattctttta cattaaatac agtaacttcg
      961 tttactgtac caaggtagta gctagtttta ctatatttag tattctggct tgaactccta
     1021 aaaaatgaaa ataaaattaa tgctaaaatc aatatcacaa aaataaatgt gatttttttc
     1081 ttattcatgg tatattcctc ctatgaaatt gtgttattat tttattataa cataatttca
     1141 taggaggaca aaaaatgaag aaaaaagaca ttcttcttat tttaggcatt ttggttgtta
     1201 ttacagcatg ttatgggata ataaatgtta taaattctaa aaatgctgga aatattgaga
     1261 tttatgtaga taataaattg tataaaactg tttcaataaa tgccaaagaa gaatttaaaa
     1321 tagagagtag aggggggtat aacatagtaa agattcatga taaaggggtt gagatagtag
     1381 atgcttcatg ccctgataag gtttgcgtac atactggatt tataaacaag ccaagtcaaa
     1441 gcatagtttg tatacctaat agagtgagca taaaaataaa tacaaatgaa aagaatgata
     1501 atcaagaaga tataatttct aaataaaata ataaaaataa ttgaaaggaa ttggtgataa
     1561 ttatggttaa cttaggaaac gattgggatg aattattgaa agaagaattt gaaaaagatt
     1621 actacttaaa cttaagaaaa tttcttatag aagaatataa aacacgccaa attttcccaa
     1681 atatgcacaa tatatatgaa gctttaaaac acacttcata caaagacaca aaagtcttaa
     1741 tattaggaca agacccttat catggagata atcaagctca tggtttagct ttttcagttc
     1801 aaccacaagt gaaaactcca ccatctttat taaatatgta taaagaatta aaagatgatt
     1861 taggttgttt tatacctaat aatggatatt taatgccttg ggcagaccaa ggagtgttac
     1921 ttttaaatac tgctcttact gttagagcgc atgaagctaa ttctcataag aacaaaggat
     1981 gggaaatttt cactgataga gtaatatcta ttttaagtga gcgtgaagac ccagttattt
     2041 ttgtactatg gggaagtaat gcacgtaaaa aagtagagtt gattgataca agtaaacatt
     2101 atattttgga agcacctcat ccaagcccat tgtcagcaag taaaggcttt tttggatgta
     2161 aacacttctc taagataaat gaaattttga aaaagctagg aaaagaacct attaattggc
     2221 agatagaaaa tatataaaag taaaaggtaa caaaatgata acaaaacaac aataaacaat
     2281 gacattgttg taactaatag aaatatgctt aaatagttga aaatactatg tatgcatatt
     2341 tctatttttt tgtcaagttg acaactcttg aaaaaatagt tacaatttag ttacacaaaa
     2401 aacacaaata aaaaaagaaa cttacaaagc agaattttat gaagcaaaat atatagtgta
     2461 aaaaatctta gggaggaaat tttatatgaa ttttaaaaaa ttgagtaatg ctaaagtctc
     2521 ggtaatggca gttgttttat cagttggaat attatcggga tactctgcgc ttaataaaca
     2581 agtaacatta gtagtaaggg gagaagaaag aactatatca acatttagtt ctaatgtagg
     2641 agacctttta gaatctcaaa acattagcta tgattcaaat gatatagtaa ctttagatga
     2701 aaatacaaag ttatctaatg gagataaaat agaagtaatt gatgtaaagg aaaaaacagt
     2761 tgtagaaact aaagacattc catttgaagt tacagttgtt gaagatggct ctttattaaa
     2821 gggtgattct aaggttaaag aagaaggaaa gtctggtaaa agtgaattaa tatataaaga
     2881 aacttatcac aatggaaaga aagtagaaaa ggaatttgtg aaaaaggttg ttaaagctga
     2941 gccagtaaat aaggttgtaa gcaaagggac aaaggtagaa atgcaagtag cctcatcaag
     3001 gggagaaagt tcaagaagaa tggcttcatc atcatctagt agcagtagta gtaacaatgg
     3061 taaaaatatg agagtggtag caactgcata tactggtcac agtataactg caacaggaac
     3121 aaaacctaag tggggaacaa tagctgtaga ccctaaggtt ataccatatg gaactaaagt
     3181 ttatatacct caatttgaca tggtgtttac tgcagaagac tgtggtggag caataaaagg
     3241 aaataaaatt gatatctaca tgaatgattc aaaaacagtt tataactggg gaagaaaaac
     3301 tatagatata cgtatattaa aatagttaaa taatttatat ttttactgga aattatattt
     3361 tgattatata gagaagaaac tctttataga ttctaaaaat ataatttcca gttttttatt
     3421 ttggatatgt aataaaacag gtaaatttta accatgtata gataaaagct atttaaatta
     3481 taatttacta tcatataaat ctataaaaat tgttgcaaag agtcaaaata tatgatatta
     3541 tagatttcgt tgagaaggat ttcattatgg gagtggatgg gtgccggtgt gccctctagt
     3601 cttcaaaact agtatgaggg gttaagagcc tcttaggtgg gttcgattcc cacgcattcc
     3661 cgccaatatg taaaaggtat cttttaatta aaagatacct ttttaaattt attttaagat
     3721 aatttatttt taataatata taatgttttg tattatataa tgtaaatttt ctaatacaaa
     3781 acattatata tttagataaa aatctaggag tgtggaaaga tggaaaataa ttttacaacg
     3841 agaactagtt ttctagttgg agatgatggg ataaaaaaac ttaataattc aaatataata
     3901 gtatttggag ttggaggagt tggtagtttt actgttgaag ctttagcaag agctggagta
     3961 ggcaatatta ctattgtaga ttttgatgat gtagatataa ccaatataaa tagacaaata
     4021 ccagccctac attcaactgt aggaagatac aaagttgatg tcatggaaga aagaatatta
     4081 gatataaatc ctaatatcaa tataaaaaaa ataagaagtc tttataataa agatactagt
     4141 gacgaaatac taactgaaag atatgattat gttgtggatg cgatagacat ggtaagttcc
     4201 aaaatacatc taattgaaac ttgtgaaaaa aaaggattga aaataataag ttctatgggt
     4261 atgggaaata aattagaccc aactaaaata gtcgttacag atatacacaa aacaagtact
     4321 tgtcctctag ctaaagttat gaggaaagaa cttagggata gaggtatcaa aaagttaaaa
     4381 gtagtatatt ctactgaaca accaatagaa cttaagaaga aagtaatgaa tggtagaaaa
     4441 gtaacacctg gtagtgtatc atttgtccca tctgtaggag gtttaataat cgcatctgta
     4501 atagtaaatg aactattagg acaataagtt ttaaatattt taatttataa attttatata
     4561 caaatttgaa ggctagaagc atgaatacaa agcaatttta tattcatact tttagcctaa
     4621 ttattttata ttaccttatt ttatgtagtt gtagttttat tttttagatt atgtatctat
     4681 ttcataatta tattttgtaa atataatttc tccatcacta taaaaaacaa tatctcctat
     4741 tctatctgtt ctataaattg atacatggtt ttgttttaga ttattaataa cctccctatg
     4801 tggatgacca tattgatttt tatatccaca tgatattgca gctatatctg gactaatttt
     4861 ttttataaat tctaaagatg aagaagaatt acttccatga tgacctattt ttataaaatt
     4921 aatattattt aatttaaaag aatgtaaaat ttctttttca ttttcttctt cagcatcacc
     4981 cataaataaa aaagaattat cattaaaagt taatttgaaa actatagaat ttaaattaga
     5041 ttcttcttgt atatatgatg gacttaatac atatatattt atattgttat ctatgtttaa
     5101 aacatcattt ttatagagat gctgaataga taagttttta tccgtacaag aatttattag
     5161 attttgataa gccttactat cagttgattg ttcaggcata tatatggagt ttacattaaa
     5221 tttttttata atattatcaa gacttcctat gtggtctgaa tctggatgag ttgctattat
     5281 aatatcaata gtttttattc ttttttgtct aagataacta ataattatat tttcgctatc
     5341 ttcatcacct ccatcaatta atatattctt atttgtgggt gtttgaacta aaatactatc
     5401 accttgtcca acatcaatca tatgtataga tagtagagat tttttgtcac agccgattaa
     5461 aagtgatatt ataataagta ttgataaaaa aattttattt ctcaataaaa aatcactcca
     5521 ttcattacat gtttatataa atttatgatg ggtaatataa gatatatatg acttattata
     5581 ttttatattt taaacaatat aaatgaaatt aataccataa taaaattgga gttttttata
     5641 tatacaaaat aaaattttta aaattaagca ataaaaaagg taggaggatt ttatgttttt
     5701 caaagatatt gaactaaatt caaaaaaaga gttagaccca tattttgatt tagtagatta
     5761 tgaagcttgt gagtactgtt ttagtacttt atatatgtgg caacatgttt ataagacagg
     5821 atattatata ggtgaagatt ttgccgtttt agttggggaa tacgaaggag atagcttctc
     5881 aatattacca cttgctaaaa aagataaact tccagaagtt gtagattttg tccttgaata
     5941 tttttctaaa aataataaaa aaatatactt aagaggtatt actactgaag tagtagagtt
     6001 tttaaaagaa aaatatccag gtagatttga gtatatagaa gaaagagatt tatttgacta
     6061 tatttatgat gcagaaagtt taagaactct agctggtaaa aagaatcaaa agaagagaaa
     6121 ccatataaac tatttcttaa aagagtatgc aggtagatat gaagctaaat tattagataa
     6181 agaaaacttt gatgagtgtt tagtactaat gaaggaatgg gaaagtaata aggaagaaaa
     6241 taatgaattt gatgaaagta tggatgatga attaataggt atcaaaaaga tatttaacca
     6301 ttatgacata ttaaaagata aggtaaaagt gtttggtgtt tatgtagatg gaaaattaga
     6361 agcttttagt ataggagagc ttttaaatcc aaatatggca ttgattcata tagaaaaagc
     6421 taatcccgat ataagaggtc tgtatccatt tataaatcaa caatttttgg taagtgaatt
     6481 taaggacgtt gagtttgtaa atagagaaga agatttaggt attgaaggat taagaaaagc
     6541 taaactttct tatcatcctt gcagatttgt tgaaaagtac agtgttaggg aggcttaata
     6601 gactatatgg agattaggta tgctaaagaa gaagagatag aaagtataaa agatatttgg
     6661 agttattgtt ttaacgatac ggagagtttt atgaagtact attttaatga taaatataaa
     6721 tctgaaaata ctgtagtagc tttagatgaa ggaaaaatta tttcttctct tcagctaaat
     6781 cagtataagt tattactaaa tagcaaggta tataatacat catatgtggt aggagtttct
     6841 acacttccag agggaagagg agctggatat atgaacaaag taatgaaatt tactttaaac
     6901 gaattgtata aaaaagggca attagtttct atattgatgc ctattgacta tagactttat
     6961 agaagatttg gatatgagca ttgttacgac cagattgaat atactattaa cacagatgat
     7021 ttaaaaaatt ttaagtcaag tggaaaaatg ataaagtcta atttatcaca aattgatgat
     7081 ttgatacaaa tagatagaac ttttttaaat gaggttaatg gtaatgtgtt aaaagatgaa
     7141 cattattatg aaaacttatt taaagagatt caaagtgaag atggattttt atacattcat
     7201 gaaggaaatg aaaaagatgg atatatagtt tattttcttc aggaagataa aatgtttgtc
     7261 agagagttat tttataaaaa tatagatgcc cttaaatcaa tgcttaaatt tatatataat
     7321 cacaatactc aatgtaagat agttactata tctactccta caatagataa gataaggttt
     7381 atattagaca accctaaaga tagtgatata aaaataaaac cttttatgat gggaagagtt
     7441 ataaatgtaa agaagtttat tgaagatata gatattgaga aggatattaa tagctcattt
     7501 aatttattaa tagaagacaa gtttatagat gaaaataatg gtctatttaa aatttctata
     7561 cagaataaga aggtatctgt agagcagtta gataaaaaag gtgcagaaaa gcctcaagaa
     7621 gattttgata ttaaattaga tataaatacc ttaacacagt tatcattttc ttatatagat
     7681 gtaaatgaag ctattttctt aaatgatata aaagatgttt cagaagaaac actagaaaca
     7741 cttaattgta ttttttctaa aaaaaataac tatataaatg aatatatata ggtagagtac
     7801 gttaaaagat tctaaaaatc ttttgacgta tttttttgtt tagataacaa tttatcttgt
     7861 ttaaaaattt tctatttgaa aataatctaa ataatatact tttatatggg agtgattata
     7921 attcaaataa gaagaccatt attaattata ttaatgtttg tggtagctat atcatttatc
     7981 attacaaata acacaaaaaa cgacgaagaa cttaatgata agataataac tatacaagga
     8041 gttgtcaaag gaaaaattga aaagaaaaga tacaatcagt acaaggtagg ggtttttcta
     8101 ataaatgatt atacaaaaaa taaaaatttg aagattggtc agaaagttaa tattacaggt
     8161 aaattcaaaa gtttagataa aatgaagtat gatgattttg attatggaag gtatataaaa
     8221 agtactggat ataaaggtat agtgtatata aataactata aaattattga taaaaataaa
     8281 ctttactctt taatagggga aataaagttt tatattagta aaacttatag gtacttatat
     8341 aagaaaaact cagattttat aaattctata ctattagcag aagttgaaaa tttgactgag
     8401 gaacaaaaag aaatttttac cagaactgga actagtcatg taatatctat atcaggtctt
     8461 catacaggaa tattatgtgt aattatatcc tttctattaa gaggtataaa taaattgtat
     8521 aaattattaa tactgtcgat ttttataact ttatattgta taatggtagg tgcgtctcca
     8581 tccataatta ggtctatagc atttgttatg attttttatt tatcaatatt tattgataga
     8641 aagaaagatg gaatatcggc attgtctcta attgggataa ttttaattat gaataacccc
     8701 tatgttatat ataatgtaag ttttcaattg tcttttttag ctacactttc tatattaata
     8761 ttctataata aaataaatag tattataaaa ttaagtatgg tatcattgac aatatcatca
     8821 aatatattaa ctttacctat aatttattat acatttaaag gtataccatt gctttcaatt
     8881 ataggaaatt taattatagt gccatttgtg ggagtaataa tgtatttaag tattgcaagt
     8941 ttaatcgtat ttaaagtaag tgtagtaata gctaaaatta tatctttttt taatagtaca
     9001 tttatagaaa gcatattttt tttactagaa aaaatcagca atttaagttt tgcatacatt
     9061 aatatagaaa atcctaaatt ttatattgtt gttatttatt acataggagt gtttttttac
     9121 atattttaca tagaaggaaa agagataaag gagcaagaga atgaattaca aggatattat
     9181 aaggaatgta aaagagaaaa actttgaaaa aatgtatctt ttttatggaa gagagtatta
     9241 tttaattgag aatgcaataa aagcatttaa agatagtcta aacgagggaa tgcttgattt
     9301 taatttagat ataatagatg ggaaggaaat agtcttaaat cagcttataa gctctataga
     9361 aacacttcca tttatggatg ataggaagat agtaataata aaagactttg aactattaaa
     9421 aggaaagaaa aagaatttta cagatagtga tgagaaatac ttaatagagc atttagacaa
     9481 tattccagac accacaacta tagtttttgt tgtatatgga gatgtagaca aaagaaaatc
     9541 attagttaag aaaataggaa acaatggaat tgtttttgat tgtgataagc tttctgatat
     9601 ggatttgttt aaatggataa aaaaatcttt tgctttaaat gatgttataa tagataattc
     9661 tcaaatcatg tattttattg aacaagaagg atacagagat aagagtagtg aaaagacatt
     9721 atctgattta gaaaatgaaa taaataaaat aagttcattt gttggtaaag ggaataatgt
     9781 aaccaatgat gtaattgata aattatctca aaaaaaagtt gagaatgata tattcaaatt
     9841 aatcgattat attggagaac aaaatgcttc taatgcaatg aaaatattaa atgatatgat
     9901 acaagaaggc gaatctgtat taggtatatt ttctatgata gcaagacaat ttaaaataat
     9961 tatgcaagta agacagttac aacttgatgg atattcaaca aaacttatag ctgataaatt
    10021 aaaaatgcat caatttgttg taggaaaagc attaaagcaa actaaaaatt tttctgatga
    10081 tataattgtg gaaatattaa actatatatt agaaagtgat tacaaaataa agacaggtct
    10141 tataagagat actttagcag ttgaaatgtt ggttagtaga tactgtaaaa gagaagcaat
    10201 ttaaaaaatc tatatattaa taaaaaataa atagcaagat atgttatagt tcagagtaga
    10261 tgtaagctaa attaagtttg atatacaatc aactataata tgtctaacta tttatttttt
    10321 attgtataaa tttacaaaat aaaaaacccc tacatagggg ttattatata taagaaatat
    10381 aaactggtaa ttaagcgttc ataccgttta atttttgagc taatttagct acttttctag
    10441 ctgcagcatt cttatgtata gttcctttag aagctacttg gtatattctt ttttgagcgt
    10501 attggaactt agcaacagca tcctctctgt ttccagctgc aacagcatct tcgaatcttc
    10561 ttattgcagt ttttatttga gattttctaa ctctatttaa agcagttttc ttttcgataa
    10621 cacttattct tttcttagct gattttatat ttgccaccgt gttcacctcc ctgtattttt
    10681 ttatcatatt catcgtcaac atattagatt ttaacagaaa taaatctaaa aatcaaggat
    10741 tattatttat atattttaat aatgtttttt attttgattt tttagactgt aaattgtgta
    10801 taaataattt gcaatatctt gtttttaaat tggtatattt tgtgttttaa atattttgac
    10861 atatataatt aatatttcat tttgtaacaa atatttgaga taatacaaac
//
LOCUS       DAMKMI010000132        10873 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMKMI010000132 DAMKMI010000000
VERSION     DAMKMI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.138"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            181..747
                     /locus_tag="PC429_003048"
     CDS             181..747
                     /locus_tag="PC429_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848531.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            complement(942..1928)
                     /locus_tag="PC429_003049"
     CDS             complement(942..1928)
                     /locus_tag="PC429_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF3848532.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(1958..2653)
                     /gene="pxpB"
                     /locus_tag="PC429_003050"
     CDS             complement(1958..2653)
                     /gene="pxpB"
                     /locus_tag="PC429_003050"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF3848533.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            complement(2673..3893)
                     /locus_tag="PC429_003051"
     CDS             complement(2673..3893)
                     /locus_tag="PC429_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF3848534.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            complement(3922..4713)
                     /locus_tag="PC429_003052"
     CDS             complement(3922..4713)
                     /locus_tag="PC429_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF3848535.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            5301..5930
                     /locus_tag="PC429_003053"
     CDS             5301..5930
                     /locus_tag="PC429_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3848536.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRSVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            complement(6044..7558)
                     /gene="glpK"
                     /locus_tag="PC429_003054"
     CDS             complement(6044..7558)
                     /gene="glpK"
                     /locus_tag="PC429_003054"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF3848537.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            7802..8344
                     /locus_tag="PC429_003055"
     CDS             7802..8344
                     /locus_tag="PC429_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3848538.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            8358..10850
                     /locus_tag="PC429_003056"
     CDS             8358..10850
                     /locus_tag="PC429_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3848539.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
BASE COUNT         3778 a         1806 c         1370 g         3919 t
ORIGIN      
        1 tattattttc tttgtattaa tttaacaata attcattttc atagactttt atttggattt
       61 aattaaattt taattgtcaa aatctactat gtattcttat tgccactact tctatgttgt
      121 gctataatta ctttgtttta agcgaatatt ttatattaat tttattattt ggaggttctt
      181 atgctaggtg aaaagatgag aaatataaga aaaagcaaga aaaaaacatt aagtgatgtt
      241 tccaaattga cagatttatc tataagttat atctctcaaa tagaaagaga tgctattgaa
      301 ccatccttgt cttcattgag aaaaatcgct gaagttttag acactccttt atatatgttt
      361 atggatgaca ataatactga tgatttagtc attagaaaag aagatagagt tatgatgaaa
      421 tttccaaaga gtgaaatgtt ttacgagata gtatctccaa tgcctacaac tgagtttaca
      481 ccttcaattt tatttgtaga gtttgaactt aaaccagaaa gtgaggatgc caaagattat
      541 atatatcatg catcagaaga aattatggtg gtaacgagtg gtgtggttga tatttgtatg
      601 ggtgaaaagg tagttagatt aaatccaggt gactcaacat ttataaaagc taatgtacca
      661 cataaaatca tcaatccaag taaagataca atagctcgtg gatatggagt tatatcacct
      721 ccaatttggc caataaaatc taaataaact caatatgaca cacctacttt ttgtagataa
      781 tataatagat ttaaatattg cttttatttt aaatttaaaa tatttactca aactaaaaaa
      841 atagctattt gactaatcta aaccatctta attatggtaa atagtaaata gctatttgta
      901 agttatatta taaattataa taaaaattgt atttgttaaa attaaactgc tatatcattt
      961 aatacttttg cctttacaac tcttatattt ttcatactct tgtagatatc ttgaatctta
     1021 tcttcttgct ctctcaatag tctatgtgct tcatatatat ctacaagttc aaattttact
     1081 acatcatttg gctttgcttg tgctaactta tacaaatcta cacttataac gttccctatc
     1141 ttagtatagc caccactagt ctgtctatct gataacatta taatcggttt tccatgacca
     1201 ggtacttgta tagctccata agaaatacca tcagatataa tatctgctcc attaaggtgc
     1261 tttatactct ctccatctag tctacaaccc attctatcta tttcattcgt aactttgtat
     1321 ttatttgata aaaatttctc tacttcaccg ttagaaaaag catcaaattg aggacctttt
     1381 acaattctta gtacgatttc tgaactataa gttggaatat attttgaact taatttttta
     1441 tttattgtaa aatctttttc ctgaataaaa gtatttatat aatctccttt tttaagtgct
     1501 ctaccttcaa atccaccaat tttagctctt gtataagttg acttacttcc cattgcaagt
     1561 ggaacatcta ttccaccagc tattgacaca taggctctag cacctgattt cacacctcta
     1621 aatgataata catcacctgg atttatttga tatgacctcc agttttgaat ttcttttcca
     1681 tttattttag cccctaaatc tcccccagtt actgctattt gcgtatattc atcaaaggta
     1741 attgtaggac ctaacattaa aacttctaaa gaagcctcat cttcagaatt tcctactaat
     1801 atatttccta ctctatgtgc atactcatcc attgctccac atccagatac accatattga
     1861 ccatagccaa ctctacctct gtcttgtatt aatgtttgga aacctcctaa ttcaactttg
     1921 aatcccatat ataccactcc ctgtactata acgatattta aatacttaag ttattttttt
     1981 cgatttctat aaattcatct aatgttattg gaacaaattt tataaaatct ccagctctaa
     2041 gcaaaatctg ttcttctcta ttttcatcat ataattttaa aggtgtccta ccaattattt
     2101 gccatccacc aggactatca ataggatata caccagtctg ggaacctgct attccaacac
     2161 tcccaccata aattttagtc cttggcactt ctaatctagg tgtagctatt ctttcatcca
     2221 taccacctag atatggaaat ccaggagtaa aacctagcat atatatcaag tactctctgc
     2281 ttgtgtgtat ttttataact tcatctacag tgagattatt gtagcttgca acgtttttaa
     2341 tatctatacc aaaaacctca tcataacata ctggaatctc atgtatttta ggttttggta
     2401 atactatact ttccaaatta tcttctattt tttttaaatt ctcaatcaaa ctatcaaaat
     2461 ctatttctaa tggattataa cttatcataa gtgacctata tgttggtacc atttctgtta
     2521 caataccttt aagattcgat atttctattg ctctcataaa tgaaattact tttttgttta
     2581 tatcttctga tatctcattt ccaaattcag caaccactgc cttatcccct gatatcaagt
     2641 atctagtttc cataaatgtc acctcttcta aattaagcaa ataaagcacc taggctgcta
     2701 agagaagtta ttcctgtata tgcagatatt aaaactacta ttaatccaaa tattaaaagc
     2761 catgtaggat gcttgtaatc tccaacaata tcttttcttt tagatgctat aagcataatt
     2821 cctaatgtga ttggaagtat caatccattt aaagctcctg ctaaaattaa taaagtagct
     2881 ggttttccta taaatgccat tataagtgtt gatattgcta taaatgctat tataaaatat
     2941 ttttcatatt tatctataaa aggatttagt gtctttaaga aagaaactga tgtataagca
     3001 gctcctataa ctgatgtaat tgcagcagac cataatacaa gtccaaagaa tttatatcct
     3061 attgctcctg cactaaattt aaaagctgaa gcagctggat tttctgggtc aagttgaaga
     3121 ccttttgaaa ccactgccag tattgctaaa aataaaagaa ctctcatcat tgtggcaact
     3181 aatataccca aaagagaaga tttagttatt tcttttatat tttcttctcc tgttattcca
     3241 ccgtctatta atctatgtcc tccagcaaaa gttatataac ctccaacact tcctccaagt
     3301 agagttatta ttgggaatat cagtcctttt ggattttctg gttttaccat tcttgatact
     3361 gcctctccaa caggaggatg atttgatatt gctacatatc caactataat tatcattcct
     3421 aatgctaaaa atttagttat cttatcgact agagaatttg aattttttga cataaataca
     3481 aatatggcta ttaatccact taatacagtt cctaatgtca tattcatatt aaacattaca
     3541 ttcataccaa gtgcagctcc accaacattt cctatattaa aagctaatcc tccaagagct
     3601 actaaaaaag ctactaaata acctaatcct ggtattattt tatttgctat atcctgacct
     3661 ctaagtcctg atacaccaat tactctccat acatttactt gagcccctat aaaaaggatt
     3721 gttgtaatta aaattacaaa tgaaaaattc ggtccaaaat cttgggtaaa ttgtgcagtc
     3781 tgagttaaaa accctggtcc aattgctgat gttgccatta taaatgcagc ccctataagt
     3841 gctccgacat cttttttatt tttagtttta tctgtaatat tttttgtact catattttat
     3901 ctccccctca tgttatcagt cttatactat gttctctgac gaacaaactt ctatgttttc
     3961 taatggacaa acttctatac tttctaattc aaaacgcttt cttatttctt tgacaaactc
     4021 tattgctttt ggattgtccc catgtacaca tatagaatct gctttgatat gtataacttc
     4081 acctgttaaa gtttctacag ttccttcttt taccatccta acaaccctat ctattgcatg
     4141 ttttgtatca tgtataactg ctccttctac atttcttgga accaaaaaac cattattatc
     4201 ataagctcta tctgcaaata cttcatttgc aaacctaagt ccaatttctt tagcagaatt
     4261 tatcatttca ctattatata gccccaacag tataatatct ttatcgactt catatatagc
     4321 ttcacaaatc cccatagcta actctttatc tttagctgcc atattgtata atgctccatg
     4381 tggttttaca tgttgtatat tacatccatt tgaacttgca aatgccatta aagcacccaa
     4441 ttgatatttt gtataatctt tagcttcctg ctttgttatt ttcatctcac gtcttccaaa
     4501 tcctattaaa tctagaaaac ctggatgtgc gcctattttc actccatttt ttttagctag
     4561 ctgtactgtt ttttccatat gactagggtc acctgcatga aacccacaag ctatattagc
     4621 tgatgaaata tattttaaaa cttcttcatc taaaccaatc ttatacgttc caaaactttc
     4681 tcctaaatca ctatttaaat ctactttata caatttatga cccctccttg ctaacatttt
     4741 gatagaataa gtagttttaa tttttaaatt ttgtttattt tatatattgc atgaaatatt
     4801 ttatcacttg tattcaattt tatttttgca actattctat tgcatttaat taatataatt
     4861 ttaccattta aggaatattt ttgcaattat tttaattgaa cattcatttg tatttctttt
     4921 tcaaaaaatc tttttaccat tttttagtta ataattaatt attttcactt aaaataaggg
     4981 ctttaacctt tatttttaaa gtatttttca tttttaatca ctttatatgc aagacagaat
     5041 catttttctt cattcatttg tttctttcat ctatataatt atcattcttt gtaagtaaaa
     5101 tatctttata tttttataat taattcttta tatagtttta agtataataa aatcatgtat
     5161 actaaatgca tgttaaaata ttattttaaa acatgcacaa tatttgtaca tattgtagac
     5221 atataaacct ataaattata caatagtatt gttaagatta tatatttttt taacataaat
     5281 tttgtattgg gggaaaaaca atggataaaa actatacgat gccaatatac caaaaaatag
     5341 cgttggatat tgcaaataaa atatacacgg gtgaaataca agaggattca gtactatttg
     5401 gacgctctgt tttggctggt aaatacaatg tatctccaga aacgataaga agatctgtca
     5461 aaattttaga ggacattggc gttgttaaaa gcataaaggg taaaggtgtt attgtactat
     5521 ctccagacaa agcttcttct tttataaaaa aatatagaga tattacaaat atatcatcat
     5581 ataaatcaac actttataat ttaatagata ctaaatctaa tttagaaaat gaaatattag
     5641 atactataaa taaaatttta gactattcta acagacttga aataatcaat cctttagtgc
     5701 ccgttcagtt caccataaat tctaattgca aatatattgg tcaaacagct gcacaaacta
     5761 aattttggca aaatacaggt gctacaatag ttgcaattaa aagaggtgaa gaacttataa
     5821 tatctcctgg accatatatt gaatttttag aaggagacat tctcttagta gttggtgacc
     5881 aacacattta caattctata ccaatgtttt tatatgaaaa cgaaaaataa attatataat
     5941 ttagtatgat tatataaaaa tgcaaaatta acaacaaaaa aagagcttca tattttacat
     6001 aaagctcttt ttaaattcaa ataaatttaa ctttgattaa tttttaaata tccaattcat
     6061 cttctttttc ccagcttaaa gctctcgata cagctttttt ccatccttta tataatttac
     6121 atctcttctc ttcactcata tttggattaa actctttttc tactgaccac tttgaagtaa
     6181 tttcatttct tccaatatag aatccaacag acaaccctgc taaataagca gctcctaaag
     6241 ctgtagtttc tactattttg ggtctatcta tatctacatt taatatatct gattgaaatt
     6301 gcattaaaaa attattatta cttgctccac catcaacttt taatgatttt agttttaatt
     6361 ttgaatcgtt ttgcattgct tctaaaacat cctttgtctg ataagcaatt gattccaatg
     6421 tggctcttac tatatgttct ttttttgctc ctcttgtaag tcctaaaatt gaacccctag
     6481 catacatatc ccaatatgga gctccaagac ctgtaaatgc tggcactaca tatacaccat
     6541 tagtatcctc taccctattt gcatacattt cactatcttt tgcagattca attatcttta
     6601 attcatctct aagccattga acagatgctc ctccaataaa tatacttcct tctaatgcat
     6661 atgttacttt tccatcaact ccccaagcaa ttgttgtaag taacccatgc ttagatttaa
     6721 ctatgttact gcctgtattc ataagtaaga aacaacccgt tccataagta ttttttgcag
     6781 taccttcttc aacacaattt tgaccaaata atgctgcttg ttggtctcca gcacatcccg
     6841 caataggtat tttagctcct gataacatgc gctcatctgt atgaccataa acatagctag
     6901 aaggttttac taatggcaac atgcttattg gtatgtccaa ttctcgtaat atatcctcat
     6961 cccattctaa atcatttata ttgtacatca ttgttctaga tgcatttgag taatctgtta
     7021 catgtacttt tccacgagtt aaattccata ttaaccatgt atctatagtt ccaaataata
     7081 actctccttg ttctgctttt tccctagctc cttctacatt atctaatatc cattttactt
     7141 ttgttgctga aaagtatgca tctattaaaa gacctgtttt ttcttttatt gattcttcca
     7201 atcctttatt tttaagttca tcacatattt ctgacgttct cctacactgc catactattg
     7261 cgttataaat aggtttacca gtatacctac tccaaacaac cgtagtctct ctttgattag
     7321 ttatccctat tgaagcaatt tcttctggtc ttatacctgc tgtttctata acttctctca
     7381 ttaccccact ttgtgacccc catatttcca tagggtcatg ctctacccat ccagcttttg
     7441 gataaatttg attgaactct ttttgtgcaa ttttcactat ttcgcctttt ttattaaata
     7501 gtatagctct tgaactggtt gttccttggt ctaatgccat tacatacttc ttttccatca
     7561 ccatattctc cttatcatat aatatttaat ttattaaaaa gaaaaattta atatcacata
     7621 tttcaataaa taatcatact taagtacaaa tttaatataa tagatattta gtaagaagac
     7681 acttattttc tatcatatct atataataat attatacatt atagcttaca tattgattaa
     7741 taaattttaa aggagtataa taaacagtgt tcataataat tttaataaaa gaggtggtaa
     7801 tatgccaaag attttagaaa atgtaaaaga agatatatta aaagtgtctc gggatatgat
     7861 tttagaagaa gattactcga atattagcat aaggaaaata gctcaaaggt gtggtataag
     7921 tgcaggtaca gtgtataatt actttaattc aaaacaagaa ataatagaat atatcacaaa
     7981 atctgaatgg gatttattga taaggcgaat tgaatactcc aataaaaaca ctgaagatta
     8041 cataaaaaaa ctaaacatta tttttactga aataagaaat tttataaaca aagttcataa
     8101 tatccaatac aatgactttt taaacacttt tgaaacagag agattttttg aaatgaaaaa
     8161 acacaaagac gactttcatg aacagctttc aaataaagta tatgaagctt tagaaaaaga
     8221 aagttttttt aataatgaca aattagtatg caatatcata ataaaaatgt ttttttcata
     8281 ttcaacatat cttcatatag aatttgaaga tttaaaaccc tatattgaaa aattaataca
     8341 ttaaggtggt gtttaccttg aatcataata ctagtattaa gaacaaggac agtaatccaa
     8401 aaatattttt agtacttaca ctttatctat taggtatatt tatgggagct attgatactg
     8461 gaatagtatc accagcaaga acgattatac aaaatagcct tggagtaaat gaaaaaacgg
     8521 gaatttggat gattaccata tacacattaa catacgcttc tgtaattcct atctctggaa
     8581 aattagcaga taaaatagga agaaaatatg tgtatctagt tagtatattt ctatttggta
     8641 gtggttcatt aatatgtgga ttatcatcac tattttccaa tttctatatt ttattaattg
     8701 gtcgtgttat tcaagcaatt ggtggtggtg gaatcatgcc aattgctaca gcagaatttg
     8761 gaactacctt cccagaaaac aaaagaggca tggctttagg tttagttggg gcaacatatg
     8821 gtattgccaa tattttaggt tcaagcattg gtagcacaat tctaagtata tttggaactc
     8881 aaaattggaa gtggctattt tttgtaaatc ttccaatttg tttaatcata cttattggtg
     8941 gagttttctg tattaaaaac aataagtctg aatctaaaga aaaaatagat aagcttggta
     9001 cattgatgtt gatatgtata atcgtatcac tattatatgg tcttatgaat atagactttt
     9061 ttaattttaa aaattctata caagatatat cagtttatcc atatttattg acatttattg
     9121 tacttatacc tattttcata tttattgaaa atagagctaa agaccctatt ttaagctttg
     9181 aatatttttt aaatccgaga atattgatag tacttatttt atctttaatt gttggtattg
     9241 gaatgatggg tatggtattc gttccacagt atgctgaaaa tgcattaaag ataaatgctg
     9301 gttctggtgg atattttgta gctatattgg gattatttgc aggtattgct gctcctttaa
     9361 gcggaagact tattgacaag tttggggcaa aaaaaatact attactagga ttttctgtat
     9421 ctatgatagg ctctctttac ctagttttaa ttgctctaaa aaccaatact gtatttagtg
     9481 tatgtgtaag tttaatattt atgggattag gaatgggatt tactatggga actcctctaa
     9541 actatatgat gttatcaaat acaagattag aagaatctaa ttcagcactt gcaacattat
     9601 ccttgatacg ctcaattggt acttcaattt ctccagctat aatgattgga tttatagcac
     9661 atgcaggatt atctgtgcaa gataatatta tggacttagt tggaaagccc tcaactccta
     9721 aaatagttca acttgaagaa cttgaaaaca tgatagatga cttaaaatca gaccctgaaa
     9781 tggcaaaaaa tttaaaaaat gtctctatcc caaatatgaa tgatacttca aatataaata
     9841 tggacatgac aggtggcaaa cttccaaaat ccttacttaa taaagttcag tcagcagata
     9901 caaccaacat taccaatata acaaaagaaa tatcaacaac aatgtttgat gaaaaagttc
     9961 catctataat tactaaaata cagaaaaatg tacaaaaagg aatagatgga acacaaagtg
    10021 gaataaacgg tattgaagat ggtgaaaata aactaaatag tggtattaaa ggagttcaaa
    10081 ctggtattga caatatggta aaagctagag ctggtattaa tcaaggaata gatggtgtaa
    10141 aaaaaggtat tgctggtgtt gacaaaggta taaaaggtat ggaacaaggt cttgaaaaac
    10201 aagatgaggc catcaaggaa cttacaactg cttataataa aatacctact acagaaaaaa
    10261 ctcctactga taacgaaaat aactcaaacg aacaaaacca gagtaatacc agtaattctg
    10321 aatcagatga cactaagaat gattctacag aaaataagaa taacaattcc agcgagaata
    10381 aaccaaatgt aaatgaacaa aaagaaagtt taaatgctca aatacagcaa ctaaaaaaga
    10441 caagagatga cttaaataca aagttacaaa aaaataaaac tcaaaagaac gaattaagta
    10501 aaaaactaag atcaatggaa tctcaaaaga aagagctaca aagcaagtta aataattcta
    10561 taagccaaaa gaaagacatg gaaaaaagcc ttgatacaat gcaacagcaa aaaaaatcac
    10621 tccaagcagt tttagcaaag acacaagagg taaaaactga aataccaaag gcttttgata
    10681 aatcaaaatt ggattatata aattccatag aaaataatag aacaaaaata gaaaacacac
    10741 tacaatctac tctaaactca ggatttaaac agatgtatat aacagtattc tgtgtaaatc
    10801 tactagcatt tataatatta ttgttctata aagaaaataa aactagataa aagagtgtta
    10861 tagtattata ttg
//
LOCUS       DAMKMI010000133        10869 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMKMI010000133 DAMKMI010000000
VERSION     DAMKMI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.139"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            119..886
                     /locus_tag="PC429_003057"
     CDS             119..886
                     /locus_tag="PC429_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF3848540.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1104..1317
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1571..2605
                     /locus_tag="PC429_003058"
     CDS             1571..2605
                     /locus_tag="PC429_003058"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF3848541.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2646..3863
                     /gene="argJ"
                     /locus_tag="PC429_003059"
     CDS             2646..3863
                     /gene="argJ"
                     /locus_tag="PC429_003059"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF3848542.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3878..4738
                     /gene="argB"
                     /locus_tag="PC429_003060"
     CDS             3878..4738
                     /gene="argB"
                     /locus_tag="PC429_003060"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF3848543.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4768..5955
                     /locus_tag="PC429_003061"
     CDS             4768..5955
                     /locus_tag="PC429_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF3848544.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYVERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            6064..7086
                     /gene="argF"
                     /locus_tag="PC429_003062"
     CDS             6064..7086
                     /gene="argF"
                     /locus_tag="PC429_003062"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF3848545.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7213..7830
                     /locus_tag="PC429_003063"
     CDS             7213..7830
                     /locus_tag="PC429_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF3848546.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(8050..8748)
                     /locus_tag="PC429_003064"
     CDS             complement(8050..8748)
                     /locus_tag="PC429_003064"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF3848547.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8910..10127)
                     /locus_tag="PC429_003065"
     CDS             complement(8910..10127)
                     /locus_tag="PC429_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF3848548.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10318..10731)
                     /locus_tag="PC429_003066"
     CDS             complement(10318..10731)
                     /locus_tag="PC429_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848549.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT         4258 a         1185 c         1811 g         3615 t
ORIGIN      
        1 aataatatta tgtaaaataa aaatatatat gacatgatgt tgttgtattc aaaatattaa
       61 gataaaaata tatagaatat atttaaaaat tattatgcaa ttagaaaagg agataagtat
      121 gcagattatt gatataaata taaataatat tgacaatgaa catatatgtt gttcaatttc
      181 tgaaaaaaaa ggagaaaatt gtgttttttc taaaaaagct tggatgaaaa ataattttga
      241 taaaggtcta gtctttaaac gatttgatgt aagaggtaaa gcttttatag aatatattcc
      301 agcagaaaat gcatggtgtc ctattactgc ggacaattat atatttatta actgtttttg
      361 gatatctgga aaatttaagg gtcaaggata ttctaatcta ttgctacaag agtgtgttga
      421 tgatgcaaaa tcaaaaggta aatatggatt agttgtgctt tcgtctagta aaaaaatgcc
      481 ttttttatct gatgctaaat atctaaaata taaggacttt tgtttagcag atacatcaga
      541 accaaattat gagctattat attttccctt tactgataat gctcctattc catgttttaa
      601 gaattgtgta aaacaaagaa aattaaatga gaatggtata gttttatatt acagtaatca
      661 gtgtccacat acggaaaaat atgctcctat tttgaaaaaa attgcaaatt ctagaggtat
      721 tgatgtaaat cttataaaat tcgaaacagc aaaagaagca caaaatgctc ctgcaccatt
      781 tactacatac agtctttttt ttaatggtga atttgtaacc aacgagatac tttctgagaa
      841 aaagattgag aaatttttag ataccaacat taaaaaattt aattaaaaag agatgtaaat
      901 attgtatttt gaaaatatat tattttataa tttaatatat tttggatgtt ttttaaattg
      961 acgaatgaaa tataaaaatt atatcgcaat ttagatacaa aatgctggaa attaaccata
     1021 tagaaaatat ttccaaaaat ttaaaaatta tattgacaaa gttaaaaaaa taaactatca
     1081 tataaaacaa taagaaaata aattgaaatc ctgtgaaaaa gagagtagca taaagctagt
     1141 tgatagagag ctgggaatgg tggaagccta gtaacgaatt ttattgtgaa tagagctttg
     1201 gaggagatta ccagaagtaa tagtatggta attggtgaat ctcgccttaa gagattagag
     1261 tgggtaagat tatcttatca attagagtgg taacgcgggt gtatattcgt ctcttggtct
     1321 ttgaccaaga ggctttttta attttaaatg tacattttgt agatactttt taatttgagt
     1381 tttaatgatt attgtaattt taccaataag agtggtaccg cggaaaatcg cctcttagta
     1441 tttataatgc taaggggttt tttgttttaa ttagagtatt ttaatctacg tattatcaaa
     1501 tgattatcat atagtaaagt ggaaagatag gattttaaga ctatttgaat ataaaaatgg
     1561 agggaacaaa atgaataaaa taaaagttgg tattattggt gcaactggat atgtcggggc
     1621 agagttgatt agattattaa tgaatcatga taaagtcgaa gtgacagcga taggttcaaa
     1681 ttcgtatgtt ggaaaagata tagttgatat atatccaagt ataggatata aaaataacat
     1741 gatatgtata gaaaatgaaa aagttataga tatgtgtgat gttgtgttta cagcccttcc
     1801 acatggagtt agtgaaaagt ttgtaataaa ggcaattaaa tcgaaaaaga aagttataga
     1861 tttaggtgct gactttagaa taaaagatga agaagtatat agtaaatggt atggagtatc
     1921 ttttatagat aaaatcttac ataaaaaagc tgtatatgga ttaagtgaaa tatacaaaga
     1981 agatataaaa gatgcagata taattgcaaa tcctggatgt tatccaacta gtatttcttt
     2041 accattgatg ccattactaa gttctaaatt gataaaaaat aataatataa taattgattc
     2101 taaatcaggt cttacaggtg ctggtagaga attatcgatt agtagtcact ttacggaggt
     2161 taatgaaaat atcactgcat ataaaatagg gaaacacaga catactccag aaatagaaca
     2221 aaatttaagt gaatcctgca aagaaaaagt aagtgtagtt tttacaccaa acttaatccc
     2281 agtaaatagg ggaatcttat caactatata ttgtacaaaa gaagataata tttctataaa
     2341 tgatatacat aggaaattaa ctgattatta tgaatttaaa gaatttatag aaattcttcc
     2401 attagataag atagcttctt taaagaatgt tagattctca aataaatgtg taatatcatt
     2461 acatgaaaat ggagatacat tgataatctg ttcagctata gataatatga ttaagggagc
     2521 agcaggtcaa gctatacaaa acatgaatat aatatttggt attgaagaaa atacaggttt
     2581 aaaaaatatt gcaccatcat tttagaaata aaaaatacta aactcatata attaggagga
     2641 taaatatgga gattataaag ggtggagtta cagtttcaga aggatttttt gcttcaggga
     2701 ttcactgtgg cttgagaaaa aataaagaaa aaagagattt agctttggtt tattcagatg
     2761 ttttatgtga tgcagcagca gtagtaactc aaaataaagt aaagggcaat cctgtttatg
     2821 ttacacagga acacattaaa aatggaaaag cacaagctat catagtaaat agtgctaatg
     2881 ctaatacctt taatggaaaa gaaggactta taaatgctta taaaatggct aaattcacca
     2941 gtgataaatt aaatattaaa gaaagtgatg ttttggttgc atctacaggt gttataggaa
     3001 agccattaaa tatagattta atagaagaaa atatggatga attagtgaat aatctaagta
     3061 aacaaggtca tattggagca agagaagcta ttatgacaac tgatattata aaaaaagaaa
     3121 tagcagtagc aataatgtat ggagataaaa aaataactat tggaggtatg gcaaaaggtt
     3181 ctggtatgat acatcctaat atggcaacaa cgttaggatt tataactaca gatgcaaata
     3241 ttgatggagt tttgctaaaa gaagctttaa aaattgctgt tgacaaaagt tttaatagag
     3301 ttagtgtaga tggagataca agtactaatg atatggtaat gattctagca aatggaaaag
     3361 caaaaaatga tagaataaac aaaaaagatg aacattatca agtatttttg agtgcactta
     3421 catatgtctg tatagaactt gctaaacttg ttgcaaaaga tggagaagga tgtacaaaac
     3481 taattgaatg ctatataaat ggagcattaa gtgaagcaca tgcagtaaaa ttagcaaaaa
     3541 cagttatatc ttcttcatta gtaaagacag ctgtatttgg agctgacgca aattggggta
     3601 gaatactgtg tgctttaggt tatgcaggtg aagaaataga tatggaaaaa gttgatatta
     3661 tttttgagag tatgaaagga tatattgaag tatgtaaaaa tggaaatgga ttagatttta
     3721 atgaggaaaa agcaaaaaaa atccttgaag atgacgaaat aagtatatta gtagatttaa
     3781 atatgggcaa tgccagagga aatgcatggg gatgtgattt aagctatgat tatgtaagaa
     3841 taaatggaag ttatagaaat taggaggata tactttaatg attaatatag aaaaagcaaa
     3901 tacacttata gaagctttac catatataga aaaacatcag ggaaagacta ttgtagttaa
     3961 gtatggtgga agtgcaatga aaaaagatgg tctaaaagaa tctgttatgg aagaccttgt
     4021 gttaatgagt tatgttggaa tcaatatagt tttagtacat ggtggagggg cagaaatcaa
     4081 taagatgcta gcaaaagtgg atatagaaag taaatttgta aatggtctta gatatacaga
     4141 tgaagagacc atggaaattg taaaaatggt acttgctgga aaggttaata aagacctagt
     4201 aaataaaata catacaaaag gtggaaaagc agtagggctt tgtggaatag ataataatat
     4261 gattttatgt gacccatata aaaattatga acttggattt gtaggagaaa tcaaaaaagt
     4321 aaatgttgag cttatagaat cttgtctaaa gtcaggttat atttcagtaa ttgcaactat
     4381 aggtgttgga gatgatggag agacttataa tataaatggt gacacagcag cttctgctat
     4441 agctaaagag ttaaatgcag ataaattgat acttcttaca gatgtccctg gtttgttaag
     4501 agagccagat gaagaaaaat cattaataac agaagttata ttagaggatg ttgataaatt
     4561 atttgaagaa ggtataatta ctggaggtat gatacctaaa atagagggat gcgtagatgc
     4621 cttaaataac ggagtaaata gggtacatat tttggatgga agagttccac attctataat
     4681 aacagagcta tttactgata gtggaatagg tacattaata agaaaagaaa atgaataaaa
     4741 ttataagttt aagaggggag aataagaatg aataacgaca atacaatttc aaaatggaat
     4801 gaatatttta tagatacata taatcaaccc aattttgtca tagattatgg tgaaggaagt
     4861 tgtttttttg atactaatgg caataaatat atagatttta caagtggata tggagtatct
     4921 tctcttgggt atagtaatag taatttaaag aatgctctaa aagagcaagt agataaatta
     4981 ttacatactt caaatttata ttttaatgag ccagtgttgt attcaggaga gaaaataatt
     5041 aattcaagtg gaatggctaa agtatatttt tgtaattctg gaacagaagc aaatgaaaca
     5101 gcattcaaaa tagcaagaaa gtatagtagt gacaaatatg gtaatggtag aggaactatc
     5161 ataagtttaa aagactcttt tcatggtaga acaatgatgt ctttaatggc aactggtatg
     5221 gacaaatacc ataagtattt ttatcctctt cccgaaggtt ttaaatatgt agaaagaaat
     5281 aatattgaag atttaaaaaa taatcttgat tccacagttt gtgcaataat tcttgaagcg
     5341 attcaaggcg aaggtggagt taatgttctt gaaaaagatt atgttttgga aatagtaaaa
     5401 atatgtcaag aaaaggatat tgtagtgatt tttgatgaag tccaatgcgg tataggaaga
     5461 actggtaaac tttttggcta tgaatatttt gatgtaaaac cagatatagt taccgtagca
     5521 aaagggttgg gagcaggaat accagtagga ggagtattag taaataaaaa actttcaaaa
     5581 gtactaggaa aaggagacca aggtacaact tttggaggta atttattagc tatggtggca
     5641 gcttcagttg tattagatga gatatctaaa gatggttttt ataatgaagt attagaaaaa
     5701 ggaaattata taagaaaatc tattgaaagt tttaataaca aagttgtatt aaagacaaaa
     5761 ggtattggtc taatgatagg aattgaaact aatattgaat ctagtattat agaagagaaa
     5821 gctagaaaaa aaggattgct tattttgact gctggaaaaa atgtattgag atttttacca
     5881 ccattaacaa tcagttataa ggaaatagat gaggctcttg aaatcctaaa agatatatta
     5941 ctagaaatta actaaatatt caagaaatag ttgattaaag ataaaaatta gaagtaaata
     6001 aaaaagtaaa gtgctaacaa gtttaattga atttaaaaag tttataagaa ggggcgaatt
     6061 aatatgaata atataagttt aaaaggtaag agttttttga cattaaagga ttttacaaaa
     6121 gaagagatta gatatttatt aactttatca agttatttaa aaactaagaa aaaagttggt
     6181 ataaaaggcg atttgctaca aggaaaaaat atagctttat tatttgagaa aacatcaaca
     6241 agaactagat gttcatttga agtagcagct catgatgaag gagcacatgt aacttatcta
     6301 ggtccaaatg attgtcatat gggtaaaaaa gagtctgtat cagatacagc aaaagttcta
     6361 ggaagattct atgatggaat agaatttaga ggctttaaac aagaaacagt tgaaagtcta
     6421 gcaaaatatt ctggtgtgcc tgtttggaat ggacttacag atgaatatca tccaacacaa
     6481 atactggcag attttttaac agtaattgaa aatgtagata aagatttgaa taaagttaaa
     6541 tttgtatatg ttggtgatgc tagaaataat atgggaaact cactgatgat aggatgtgct
     6601 aaaatgggaa tacatttcgt agcactagca cctaaggaac tttggccaaa tgaagaattg
     6661 gttaaggaaa tgaaggaaat agccaagaaa tccaaaggag atataatgtt gactgaaaat
     6721 attgatgatg ttaaaggtgc agatgttata tatacagatg tatgggtttc tatgggagaa
     6781 gaggagcaat atgaagcaag aataaatcaa cttaaagatt atcaagtaaa tatggatatg
     6841 ataaagaaaa ctgaaaatga aaatgttata tttttacact gtcttccagc atttcatgat
     6901 ttagaaacta aggttgctca ggagatttat gaaaagttcg gattatcaga attagaggtt
     6961 acaaacgagg tatttaattc aaaatattca aaggtatttg aagaagctga aaatagaatg
     7021 catacaataa aagctgttat ggtttctact atgactgatg taaatatgaa caataattta
     7081 atatagatat gttttaatat agactttact ttaatgaaaa tttataaaat attttaaata
     7141 tattattcaa aaactctcat tatatgttaa aataacttat gtatcaacaa attaagaggg
     7201 aggatttgag atatgaaaaa aatcatatta gctttaacaa cttttttatt agcaatatct
     7261 ttaacagcat gttcaagtgc aaaaccagaa gatacaattg atagtttttt taacagtgcg
     7321 aaaaagtttg actttgaagg catgaataag gtaatggaaa ataatgacga aaaatataag
     7381 gatatattaa aggaattaga tacaaaagac ccaaatgccc aatatgtttt agattatcta
     7441 aaacagaatg catctaaaat cacatataca ataaaagaca gcgaggttaa aggtgataag
     7501 gctacaatca aagtagaatg taaatttata gattcaacac cattacttca agaaatagta
     7561 gctgaggcat ttacaaagat gattggtatg agtttttcag gacaagactt aactgatgag
     7621 aaaacaactg aaatgttagt atcaattatg aaagaaaaac aaaaaagcgt taaagagact
     7681 tatgttacaa aaaatgtaga gtttgaatgt tctaaaaagg ataataaatg gataataagt
     7741 tcagtaaatg atgcagtagc tgatgtattg ttatctaatc ttgttacagc aggacaagaa
     7801 ttttctaact ctatgaatac acttgaataa tattgtttag gtgtagtaaa gtaataaata
     7861 tatgattatt tttataaata aacatataaa tatttatgaa ggataccatt tttggtatcc
     7921 tttttattaa tataatgaaa tacatggtta atttctatat tgtcaagaaa ctatgatata
     7981 tgttataagt ctatactatt taaaataaac ttataacata tattttttat aatatagtgc
     8041 atgaaaaatt tatttaacta attttagata tttattgaca actgattcag ccaagcattt
     8101 tgatgaatca acaatattta ttgaaggata tgatttttct aaaactacat ttaaatcagt
     8161 acaagcaatt actatatttt ctatagattc atttttaaca tcttcgataa gtttattcca
     8221 aatatcaata ttgtgtggat tttctttatc catttttata ttttgaataa gattgtttaa
     8281 ttttatttgc cattcatctt taaatataaa ttcatgtcca ttatgtatta taccattctg
     8341 gtaaattgta gactcaaaag ttgaactagt cgaaaaaaga gttacttttt gtgagatagt
     8401 aggtaatttt ttaactgttt cttctacaat atttaatatt ggtatattaa tagactcttt
     8461 taattcttta aagtatatat gtgctgaatt gcatggcatt gcaataaaat ttacaccagt
     8521 tgattcaagc ttttgtaatc catcaattat agtttctttc ataagttcat gatttattgg
     8581 acggtcaata taaaatgggg taggtaatga atatatcatc atctttggaa attcatcatc
     8641 atattttgct ccatattgag attgacactc atcaataatt aaatcaacaa aaggggctgt
     8701 tgaccttgga cccattccag ccaaaatacc aattaatttt tcattcataa aaattatctc
     8761 ctttatatat aaatatattt atgtataaaa taaaaatcta taaaagcata aaattatatt
     8821 ttataataga ttatattgta cacttatata atataaatat aaagtgattt tttagattat
     8881 atattttaat atactttctt atataagaat tattttaaat caagattatc tagtattttt
     8941 aaaaatattt ttacagcagt atccaagtca tctacagaaa cactttcatt aggattatga
     9001 cttacgcccc cagcgcatct tataaatacc ataccaatat cagttatatt atccatttcc
     9061 tgagcatcat ggccagcacc actataaata taaaaaggat taagatttaa atctataaaa
     9121 cttttttcta tgatttttgt tattttatta gaacatggta caggaacatt ttcaaaagca
     9181 agttcacttg tataattaag ttttctattt tcacatatat gtgatatttc attaaatatc
     9241 ttttctacag aattatcaag aatttcttgt gaacaagagc gtacatcaag agtaaattca
     9301 gctctttcag gtaccacatt tgatgaagat ggatagaaat tcatctttcc tacagtagca
     9361 accaaatcag cagtcgtttt agctactttt tcgatagcta atacacattc acaggcacaa
     9421 catccagcat caagtctcat attcattgga agtgtaccag catgtccaga cttaccattt
     9481 actgatatct taaaactttt aaatcctgta atacttgata caattccagc aggaagagat
     9541 tcattttcta aaacaggacc ctgctcaata tgaaattcta aatatgcatc tacatctttt
     9601 ctaggatgca aatttgttaa gttttttgta ttaaatccaa actttgttac agcttcttca
     9661 agtgtaattc cattttcatc aacagattta agaagttcct ctttaaaagt accagcaaaa
     9721 actttacttc ccattaaaga agtttcaaat ctagttcctt cctcttcagc gaaagcaatt
     9781 agcttaatat tgaaaggata agacctatta ttgtgttttg cttcttcaag accaactaga
     9841 ggcactatta tacctaacat accatcaaat atacctccac actttacact atcttgatga
     9901 gatgcaacta ctaatgtggg agcatttgga aagcttgatt tatatgtacc tataacgttt
     9961 ccaatgtcat caatttcaat atcaagattt aaaccttcca tccatgaact aattaaatca
    10021 atagattttt tatgttcttg agtcaagata agtcttgtaa gaccatttgt tgttgaactt
    10081 atttttccta atgtttgtag ataatccata catttttttc ctaacatatt tacctcctaa
    10141 aaatataatt tttatttaag ctaagattag cttttatact tataattttt gaactttcaa
    10201 ataattttgt atgtatagta aaatactatt ttagtattaa taatgatata aaaatataaa
    10261 gtctgtagac gaagagcaga agttttaagt taaaaatata aaatatgatt tttattatta
    10321 tagtattttt ctaaaataat atctctttcc agttagagga taattttcaa gtgaaaatac
    10381 tttatggtat ccaaatttct cataaaattt aggtgcttga aaactgaatg tatctaaaaa
    10441 aacatattta caacctcgtt ctttagcaat tttttcagcc ttatataaaa gttgacttcc
    10501 agtaccttta tatctaagct tctcactaac ccataagtat tttatagaaa gccaattacc
    10561 atgagtaaca cctataaaac ctgcaacaat tttacctgct ttatctcgaa tatatattcc
    10621 taaatccact gggtttttat cttcaagtct agcaagatta tattttaata atccttgaaa
    10681 aatagtatct tcatcttgtt tttctatttt ttctgtaatt tctaattcca tatttgaact
    10741 ttctccttgt ataatctttt gtatattggt attttttaat aaaatcagta gaaagaatat
    10801 agtttaacat tattctaata ggcatttatg ctaatgtcaa actaataata cttattctta
    10861 attttatat
//
LOCUS       DAMKMI010000134        10806 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMKMI010000134 DAMKMI010000000
VERSION     DAMKMI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.140"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(327..1334)
                     /locus_tag="PC429_003067"
     CDS             complement(327..1334)
                     /locus_tag="PC429_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848550.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEITVMKEDMSEVKQ
                     EINVLKVDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEM
                     TVMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSV
                     K"
     gene            complement(1564..2649)
                     /locus_tag="PC429_003068"
     CDS             complement(1564..2649)
                     /locus_tag="PC429_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF3848551.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2685..3998)
                     /locus_tag="PC429_003069"
     CDS             complement(2685..3998)
                     /locus_tag="PC429_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3848552.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLNENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDTKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(3999..6290)
                     /locus_tag="PC429_003070"
     CDS             complement(3999..6290)
                     /locus_tag="PC429_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3848553.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6280..7074)
                     /locus_tag="PC429_003071"
     CDS             complement(6280..7074)
                     /locus_tag="PC429_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF3848554.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7113..7559)
                     /locus_tag="PC429_003072"
     CDS             complement(7113..7559)
                     /locus_tag="PC429_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF3848555.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7631..9037)
                     /locus_tag="PC429_003073"
     CDS             complement(7631..9037)
                     /locus_tag="PC429_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3848556.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9055..10425)
                     /gene="hydA"
                     /locus_tag="PC429_003074"
     CDS             complement(9055..10425)
                     /gene="hydA"
                     /locus_tag="PC429_003074"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF3848557.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10558..>10806)
                     /locus_tag="PC429_003075"
     CDS             complement(10558..>10806)
                     /locus_tag="PC429_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF3848558.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIKTIG
                     FGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSVYGK"
BASE COUNT         3146 a         2173 c         1125 g         4362 t
ORIGIN      
        1 tattcatatt actatttata atttttatag agcaatttta gtgccaatat ttgcatacat
       61 ttgtatagta taaaattttt atgttctcat tgataaaatt ttacttattt caagcgttct
      121 attagatttt atcatctaaa ttttaggttt tatttttata aattaattgt atttacataa
      181 aaaaaaagac ctaaaaacaa atttttaacg gtcttttctc aattttttat tttcatttct
      241 aaaatttata ataaatagta agaacactat aaattctttt atataaaatt cttagtttgt
      301 ggatgataaa ttagtgaatt aaaatactac tttactgatt ttagtttgac aatatcactc
      361 caatttttac tagtggctaa ctctactaaa tttaagtctc ttcttatttc agctatatcc
      421 tcttttatag aattagtgtc accttttata tatgctatgt tatgttctat attatcatga
      481 gtagctttat ttatttcaga attatgtaat aaacttccta aaatattatt ctgttctatc
      541 tgtatctctt tcattgattt atcttcacat tctagactat caatcttaat ttcaattcca
      601 tataattttt catttgctat ggatacctcg tccttaattc cattcatatc ttcattaatg
      661 ttatttattt tattttgcat aaagtttact tctgatttaa cttcggaaac atcttctttc
      721 attacagtta tttcttgtct cacttctgac atgtcttttt tcattacatt tatctcttgt
      781 tttacctctg atgtatcttc tttcattaca gtcatttctt gtcttacttc ggacatatct
      841 tctttcatta cagtcatttc ttgtcttact tccgacatat cctctttcat tgtatttatc
      901 tcttgtctta cctcagacat atcctctttc attatattta tctcttgtct tacctcggac
      961 atatcttctt tcattacagt catttcctgt tttacttcgg acatatctac ttttaataca
     1021 tttatttcct gttttacttc ggacatatct tctttcatta cagttatttc ttgttttact
     1081 tccgacatat cttctttcat tacagtcatt tcttgtttta cttccgacat atctcctttt
     1141 aatacattta tttcctgttt tacttccgac atatcttctt tcattacagt catttcttgt
     1201 tttacttccg acatatcttc ttttaacaca tttatttctt gttttacttc cgacatatca
     1261 ttttttatgc taactacatc ttcatttata gtatataata actctaaaat cttctcttgc
     1321 acttgcatca ccatccctat taattattat aacattttac acaaatgcta tacaatgtta
     1381 acatatatat cagaattatt aaaattttaa ttaaacaaaa ttgattcact ttattacttc
     1441 aaaactaatt aatatctaaa ataactacaa ataaacaaaa ataaggtcaa caacaaaaaa
     1501 ggcttatctc tttgagatag cctttcttat ttttatttaa aactttgttt tttataaaat
     1561 aatctataca ttcagctctg aacatgaaat ttttcttcca agtgcttttg gtttacaaac
     1621 tgatatacaa agtccacaat atctacactc ttcttcattc aattccatct tatcattcaa
     1681 ttttctcgct tgatatgcac aaactctctc gcacatctta catttagtac atttttcttc
     1741 atcataagta aactcaaagc taagtctttc attaacatct cccatatgat tatgtactat
     1801 accacaagca ttttctatag tattatatcc taatctatta agattagatt ttaaatcatc
     1861 atgtatttta gatattactt gtgcgccttt taaaattact actgaacata taccaacaaa
     1921 gtcagcacct gccataatca tttctaaggc atcatctcca cttacacatc cacctaagcc
     1981 aattattggt atattaactt gtttttttat ttcatatatt ctctgtaaag ttattggctt
     2041 gatagtttct ccacttaaat atcctattcc atttccacct aataaaggtt gccctgtttc
     2101 tatgtctatt ctaaatgctg gtcctacaga atcacaagca gttattgcat ctgctcctgc
     2161 ttcttctaat tttttagcaa aatcgcctat ctcatctatc ataggaggaa gtttaactat
     2221 tacaggtata tttactcttt cctttgcatc tttaagcatt ggtatcaaat ctctatagtc
     2281 ataagatact aattcaataa agtcagctcc tgcatttgca actttctcta ctaattcgct
     2341 actttcttca attgtatgac ctactgaagc tatacagaca gtcccatctc ttttcatttg
     2401 tggtatttca aaatctatcc attgctctgg ctcaaaatct gtccattttt cattatttaa
     2461 aagagcatta gctgtatttc taaccatatg tggtgctgga tttatagctc tttcttttct
     2521 tatagtctta gtcacaactg cccctgctcc tgcatcactt aatatcttgc atccagctgc
     2581 actatatgtt aatggtcctg aacctattat aagtggtgat tttaattctt tacctaaaaa
     2641 attatacatt atctgcttcc ccttttcata tatatttaca ttcgttaaaa tttccaaaat
     2701 tcacaagcaa cttctttcat ttctttaaca atagcttttt tatctaaatc aactaactgt
     2761 ttttctttta aaatatgtct accatctatg tatacatttg atataaattc ttttttaccc
     2821 tgaactacta tctggtcaaa tatattatca agagtcacag gagttttaaa ttcatcttcc
     2881 ataactatta tatcagcctt attaccaact gctattttac cagaattttg aagtcctaat
     2941 acataagcac catgctctgt tgccattcta aatactaaat ttgctggcat tactgatgca
     3001 tcttcttcaa ctgatttatg cattaaaaat gcacctctca taacttcaaa aaaatcattt
     3061 atatatccgt cagtacccag tgccactttt ataccttttt taatcatttt tggtactgga
     3121 gaaaaacctc cacctacttc acagttgcta agaggcatat gaaccacttt tacacctttt
     3181 tcttttaata tatctatttc ttcatcattt acttttacac actgagatgc taatacagtt
     3241 tcatccaata tatctaaatc attatagtag tcaactgctt ttttgccaaa atgtttttca
     3301 gcatagtttg gctcatatat actctcacat aaatgaaatt gcattggtgc atttaatttt
     3361 tttgtatctt ctttagcttt cttaataaat ctatcagaac atgtgaagct tgtatgtgta
     3421 cacattattc catttattaa tttactattt tctctactcc actttattaa attagaattt
     3481 tcatttaaac atctaagtcc attttcaaag cttattcttt cacaactttc taaggataaa
     3541 atcgctttca tgcctatttc ttctagtatt tttccttgct caattaaagt tccttcttct
     3601 gtatttggag cttctagggt atcacaaaaa gctaccacac cagattctat aagttctatt
     3661 gctgatgctt tagttgttgc cttcacttct ttcaatccaa ttctattttc tataaatggc
     3721 caccaatagt catttaagaa accttcaaag tcggtaaaat gtacatttgc cggtattccc
     3781 ctagaaagaa ctccatattg atgcatatga ccattgacga atcctggcat tattatagca
     3841 tctgatttat ctattatttc ttcaatattt ttatatttta tttcaacttc ttcatttggc
     3901 aatatatctt ttataatgtc attttctact acaacagcag aatttgtata tatttcatca
     3961 gcagaagata ttaagtattt acttttaata gcaatcattt atttacctcc ctgatttaat
     4021 tttttcatgg ccatataaac tttctcagaa gttataggaa gttctcttat acgaactcct
     4081 gtggcattgt atattgcatt tgctattgct ggtgcaggtg tatgaataac tatctctcct
     4141 aaagatttag ctccatatgg acctgttggc tcataattat ctacaaaatc aacaattatg
     4201 tttcctatat ctttttttgt tggaactttg tattgtaata gattagaagt ttgaagcttt
     4261 ccatcagaac catgtctagg gtcttcatac attgcaagcc ctataccttg agttacacca
     4321 ccttcaactt gtattctagc aagtgcagga ttcataactg taccacaatc tgtagaacaa
     4381 gcataattta ttactttaaa ctcccctgtt gttttatcta actctatttc agcaaatcct
     4441 gctaagaatg gtttagcact ctcttgtata ccataagatt cagatgaata taaaacttct
     4501 tgatttccac caccaacaga ttcttttcct aattcactta acaaaacaaa cttctctaca
     4561 tcttcactat aacaaactct gtcttcatat agtattaaat catctattgg taaatcgagt
     4621 ttcttctgtg ctacttttaa tatcttttgt tttaaacttt cagcagattt tattgtagca
     4681 tttccagtta cataagtagt acaagaagcg tatgcaccag tatcatatgg acacatatct
     4741 gtatctcctg tataaacgga aattctatca atagatgtat tcaatgcttt tgcagctatt
     4801 tgtgcaagta ttgtgtcaga accagttcct aagtctgaag acccagaaaa caacttataa
     4861 gttccatctt catccatcct catatttact attgccatgt ctatgttggc tataccagaa
     4921 ccatgagttc ccacagacat tccaacagcc ctcaccttat tgtttccaat atccctaaca
     4981 gggtactttt catcccatcc aataagtttt ttacctctaa ttatacattc atcaagtgca
     5041 caacttctta ttggaaaatt cattattccg ccttcagttt gtttttttat aatattcttt
     5101 aactttattt ctgttgggtc catattaagt ttatgcgcta attcatcaat tgcacaatca
     5161 agtgcaaatg taccttgtgt tgctccatat cctcttaatg ctcctcctgg aactaaatta
     5221 gtatatacag ttctcccatc aaattttatt gcaggtacat tattatatgt tggaagtaca
     5281 ttatgacctg attcagagca aactgaaggt ccattatcgc cataagcgcc tgtattgttc
     5341 aatgctttca tatctatagc ttttatgttt ccttctttat ctgacccaat ttttatatca
     5401 aaaaacatct gatgtctagt attagtcatt gcaaaagttt cttctctagt ataaattagt
     5461 tttgctggtc tttttgtcat ccaagttaca aatgatgcat atatctctgt tacagctatg
     5521 ttttttccac caaaacctcc acctattctt ggttttatta ctctcacttt tgaaagaggt
     5581 attcccagtg ctttagaaac ctgtcttctc atatgataag ctgattggtt tgcagaagtt
     5641 actactaatc tgccattcgt atctatatac gtataagctc tatgagtttc catcatacaa
     5701 tgggcttgtg attgagtcat ataagaatgt tctactatta catcactttc tttaaactct
     5761 ttttctacat cccctttacc cattttttct ctcgatacaa tattttttgt agcatccaat
     5821 ccaaaatcaa atggacaaaa caaatcatct tcttcatgga ttaaaatttt atttccttcc
     5881 gattctcttg catccaatat aggagtcatt acttcatatt caacttttat aagtttcata
     5941 gctttaatag cagccctttc atcttcagcg gctattatag caacttcatc tcctacatat
     6001 ctaactacgt cttcaagtat taattggtca tgaggtgatg cttctggata tgactctcca
     6061 accattgaat atctataatt tggaacatct ttgtaagtga atatgtttac tacacctttt
     6121 acctttaatg cattttttat atctatgttt tttatcttcg caaaagcatg tggacttcta
     6181 agaagcttaa ttgttaaaac atctttatga aaaattaaat cttctgtata tattggtttt
     6241 ccagtaacaa tggcttcact atctattttt cttacctctt tatttaccat cacactcacc
     6301 tccaatacta ttatacattc tcactactaa ggcttttgcc atatcttttc tgtattcttt
     6361 acttcctctt atattacttc caaatgaaag ttcttcactt gatacttcac cagctttttc
     6421 tatatcattt tctaaactta atatttcact tgctttatag gcaatttttg ctcttcttgg
     6481 tctagcgcct atcgcaattt tataaatact tccttctttt aacattgctc catttaatac
     6541 tgcaaaatca cctgtacatt ttcttataga atcaaataca cctatagcat cttttttagg
     6601 taatataact tctattaata tatctttttc aagctcattt tctaaaaaat cagctaagtt
     6661 cataacccct tttttatata acttaacctt tgcatctacc actagtaatg aaggtattaa
     6721 atctgaaaat ccatacttag caaatacgct agctcctact ctagctccac ttctaaattg
     6781 aactcctact atattagaaa cagcgtttga aattacccca ttacagtaat ttttaataac
     6841 cttatttaat tctaaagttc ttaaacttgt atctgctcca attaatatgc tatcatcagt
     6901 ttcatttata taatctaaag ctaagtcttt taaatctatt gctgaaccaa tatttttact
     6961 acctagtttt aaaaatccac atcctccaag tattatgttg ttttttcttg atattagcag
     7021 ttcataagct tcatctaaac tttttggaac tacatattcc tttatagtta tcatactctc
     7081 tccccatatc aacttaattt tcacttaaaa gtctaagtgt ttaaatattt atgtattgct
     7141 cttaattgac ctttatatcc tgtacatcta caaaggttac catttacata atctataact
     7201 tcttcttctg ttggattttg aagctctctt ttcattgcca agaccatcat tataaatcca
     7261 ggagcacaaa atccacactg gtcagcccct tcgtctgcaa gaaaatctcc aaacttcttt
     7321 gcttcatcca agacaccctc aagtgttgta atatgctgac cttctactct agcagtaaga
     7381 taattacatg aaagcacggc cttgtcattt accatcactg tacataaccc acatgagcct
     7441 gtatcacacc cttttttaac acttacatat ccattttctc ttaatgtatc tgctaaaaaa
     7501 tcatctggtc ttatacttaa ttctttttct ttattattta tggtacattt gataatcaaa
     7561 taaatcacct caaaactcat atttttaagc taaagtttta ctatactttt ttatgaataa
     7621 attacattta ttaataaagg ctattccatt tatgaccttc cattctattt tttatagttc
     7681 ctcttacaca aagtctctca gctgtatctt gagcatcttt taatgctttc tcttcattag
     7741 ctgtcaatat ttttgaatct tccattatta tatttccatc tactataaca ctctctatat
     7801 ttttcatact agaagaatat actaatgtag ataccggatt atgcataggt atcgcttttg
     7861 gtgaaagcat tggattaaat attaataagt ctgctttttt acctatctcc aatgaaccta
     7921 tctcatcttc cattccaata gctcttgctc catctatagt tgccaactca agaacttttt
     7981 ctgctgacat tgctaatggg tcacacttat taactttatg ttgaagtgct gttaatttca
     8041 taagttcaag catatcttga gagttattac tagcggctcc atctactcct aaactaactg
     8101 ttattccttt tttaagcatt tctggcactg gtgcaacacc tgaagataag tacatattac
     8161 ttgctgtatt atgagacact ttcatatcgt attttttagt aagttccata tctttttcag
     8221 ttagatacac acaatgtacc ataagcacat ttggtccaag tattcctaat ttttctaaaa
     8281 catctatgtc atattgccca tgcaattctt ttgctgcttc tctatcaaaa ggagtctctg
     8341 atatatgtac tgtaaataaa gcatcatcat attcttttac tactctccat agcatttcaa
     8401 gcatttcttg agaatttgac catattgctg caggagctac tcctattttg attcttccat
     8461 tttctgtatt atggtgtttt tcaaacaatc ttcttacatc tttttcaacc gtttctacat
     8521 cttgcattat tccaggatga actccaaatt gagctcctgt attcatacat cctcttccaa
     8581 gtattcctct tattcctaat tctttataag catctattat tccatcacaa agacctggct
     8641 tactatgagg atacatgtag tcaaccattg ttgttatacc acttctaagt ccttcaatac
     8701 atccaagcat tgcagcatca taagtatctt ttggctctaa atagttagct gctggaaatg
     8761 tcatagtttc taaccagtct ttaagtacca tatcatctcc caatcctttt aataaagttt
     8821 ggaataaatg attatgtgta tttataaaac ctgggaataa aactttacct ttagcatcta
     8881 taattttttt aacatcagta tatttacttt ctatctcttt agaatttcca atatcagcaa
     8941 tcttgttatc ttttactact aatgccccat caaaaataac ttctctctct ttattcacag
     9001 ttactattat ggcattctta ataagtatat ccatcattct tatttccccc cgcattattt
     9061 agtaattttt cttttaatat attgacctgt tggatgttct ttaacattac aactttgtac
     9121 tacatgattt cctctcaaaa atacatcttc aactttacat ttaaccttaa atccttcata
     9181 tggagtataa tctacatttt gtttttgagt tttataacta attgtttctt ctttattgat
     9241 atttaaaatg actatatctg catcacttcc tacagctatc tcaccttttt taggatacat
     9301 tccaaatatt tttgcagcat tcgtagaagt aacttccaca aacttatttg cacttattct
     9361 attttctaag actccatatg tatataaaag tgctaaccta tgttcaacac caggtgctcc
     9421 atttggtatc ttactaaaat catcaattcc caagtctttt tgccccttaa aattaaatga
     9481 acagtgatct gtgccaactg tttgaatatc tccactagac aatccttccc aaaggtaatt
     9541 tatgtcttct ttttttctta aaggaggaga cattacatac ttagcaccct caaatttatc
     9601 cttattatat aaattgtctt ctaataatag atactgtggg catgtttcta ctatcatatc
     9661 tatatttttt tctctagcca tttttacagt ttcaagtgac tccctacaac ttaagtggac
     9721 tacataggat tttgaattag ataatttagt tatatcagca agtcttgaaa cagactcttt
     9781 ctcaactatg ttaggtcttg taagtggatg atattttggc tccaaattac cattttgtat
     9841 attttcttct atcaatacat ctagtaaatc cccattttca caatgaaatc caactataca
     9901 acctaaatcc cttgctttct ttaaaaccct gtatattgtt ccatcatcta ctttcatgcc
     9961 atcatatgcc atatacatct taaaagatac tatcccatcc gaaataagtt tttccatatg
    10021 attaaatgta tctttattta aacatgtaat tgtcatatga aaaccataat cacaataaca
    10081 atttccactt gcttttttat gatatgcttc tacaccttgc agtaggtttt ccccctcatt
    10141 ggcttctgca aaatctagta ttgtagtagt tcctccagct atagctgctc ttgttcctgt
    10201 ttcaaaatta tctgcagttg taatagaacc tgcattcata tcaaaatgag tatgtgtgtc
    10261 tattccacca ggaattacaa ttttacctgt agcatcaata accttatcac catttatttc
    10321 taagttattt cctatttcaa ctatctcttc atcttctatc cttaaatcac ttatataagt
    10381 cttatcagag gtaattatag ttccacctct tagtattgtt cccatatata cctcctaact
    10441 tttaagcttt aaagtatttt ctaagtattg taaaacttta tttaaaattg tattttctaa
    10501 aggtgctata ttcagcacct ctagactata tttaaataat ttattgtgaa ctaaatatta
    10561 ttttccgtat acagatttta atattccata gtaaccttct gttcctataa ataactgttc
    10621 ttgctctata tactcatcta ttgtgtgagc aagattttcc ttagaaggac caaatcctat
    10681 agtttttatt ccagcttcac ctgcatagtg acttccattc gtacagaatg agtattgagt
    10741 tatttctgaa tctattccag cttcttttaa tcccttatat gctgcttgaa caaattcatc
    10801 ttcttc
//
LOCUS       DAMKMI010000135        10372 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMKMI010000135 DAMKMI010000000
VERSION     DAMKMI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.141"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            329..1759
                     /locus_tag="PC429_003076"
     CDS             329..1759
                     /locus_tag="PC429_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3848559.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTMNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1862..2965
                     /locus_tag="PC429_003077"
     CDS             1862..2965
                     /locus_tag="PC429_003077"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF3848560.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2980..3216
                     /locus_tag="PC429_003078"
     CDS             2980..3216
                     /locus_tag="PC429_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3848561.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3233..4282
                     /gene="vorB"
                     /locus_tag="PC429_003079"
     CDS             3233..4282
                     /gene="vorB"
                     /locus_tag="PC429_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF3848562.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALTSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4284..5024
                     /locus_tag="PC429_003080"
     CDS             4284..5024
                     /locus_tag="PC429_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF3848563.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5027..5566
                     /locus_tag="PC429_003081"
     CDS             5027..5566
                     /locus_tag="PC429_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF3848564.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5690..7789
                     /locus_tag="PC429_003082"
     CDS             5690..7789
                     /locus_tag="PC429_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF3848565.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKVAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIENRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8056..8571)
                     /locus_tag="PC429_003083"
     CDS             complement(8056..8571)
                     /locus_tag="PC429_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF3848566.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YVAPTNPPVTI"
     gene            complement(8663..9874)
                     /locus_tag="PC429_003084"
     CDS             complement(8663..9874)
                     /locus_tag="PC429_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF3848567.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
BASE COUNT         3944 a         1257 c         1876 g         3295 t
ORIGIN      
        1 attcttgaat aaaattataa aaaattaaaa tcactagaaa tatgataaaa taatttaaaa
       61 tacgcatttc aatgacattt caaaacaata aaataataaa aaacaaacct aaattaagtc
      121 taaatagctt tttagttaat tttagtagta aaaaataaaa cttaaaaagt gtttgacaat
      181 tatgaatttt gagaatataa ttaaattaaa attatacaca tataaaaata tactcctgaa
      241 tcaagaatga gatatttaag attcaaaata tatattatta tacgtgaata ataaaaaagt
      301 aagaaaattt tataagggag gggtagatgt gagtagtcaa gaaagcaaac caaaaggaaa
      361 tcttatatca aattttagag atgatatgag agaccctaag aaaagagcaa tatacacata
      421 ttcaagttct ataaacttag cgtacaattt tataggttac tttgtatcaa ctgttacaaa
      481 tattttttta actgatgtgg caaaacttgg tgtaattatg gcaggtggta tgcaaattat
      541 acaatctttc ataaaaattg ctgtagctcc tataacaggt acagtatttg acaaacaacc
      601 attcaaaaaa ggaaagtatt atccatggct tagatatgca ccagcagcgt tagcattaac
      661 atatggaata tttttcatag ttccattaat gaaaataagt ccagcattac ttttaccttt
      721 gatgacagct ttattattaa tagcatcttg tgctcagcaa gtagttttca ctataattta
      781 tgctatttac cctgtagttg ctaagacacc tagagaccga gttataggct caactactat
      841 gaatatattt aaagaagttg gtaaattctt agtaggtttt tcatatccgt tattattagt
      901 tttatttgca gatgtattag gtggagaagg aatgggttat ttaggtacct acttattctt
      961 tgcagcagga tgccttttag tattttggtt ttcttcaaca gagataataa aatctggagc
     1021 agaagaagaa gttcttcaaa aagaagagaa gccaaagatt aaagcaagtg aaatggcaaa
     1081 agctttattc actaatgtac cacttatgat ggcattttgt ttagaatttc ttatatgtat
     1141 aagatctata ggattaggac ctttggctcc atattatttt aaatatgtaa tagaagatga
     1201 acgtggatta gcagtatttt tatcaatcat gcctttggta agtgtagcat tcatgttttt
     1261 cgcaccagtg tttataaaaa tctgtagaga gcaaaaacta gcttcaataa tttctttttc
     1321 tatatgtgca ctatgccact tattggttgc atggactcct tgggggaaaa ctactttagg
     1381 agtaactatg ttgctggcaa ttggtggagg tttttcaaat gtagtatcaa taataaatct
     1441 taatttcttt gccggttctt gtgattatgg gcattggaaa tcaggaaaag acttacctgg
     1501 cttaagtatg tcactatatc cagttgcaat acaagtagga gtattacttg caactactat
     1561 aagaacagtt ttaatgaact caatgggata tcaagctgat atggtagtca cagaagcagt
     1621 taaatcaggt tttataaata tgatttctta ttctatggca atacctttaa taatagctgt
     1681 tgttatagca atactatatc cagtaagtga caagaaatta aaccaaataa gagaagagtt
     1741 aaatcaaaga aatgcttagt taaaagaaag ttaagtagat ggttaattta agctattaaa
     1801 gtaagtagac gcaatagcgt ttatataaat gaaaatacat atacagaggg ggcttttaaa
     1861 aatgatagaa aaattattga gaaataccgt aaaagaatta catcaatatg ttccaggtga
     1921 accaatagaa aaagttaagg aaaaatatgg tgtaaaagaa ataataaaat tagcttcaaa
     1981 tgaaaatcct ctaggaccat caccaaaagc aatagaagct atgattgaaa tgttaaaaca
     2041 aggtcaactt tatccagagc cagaagctaa cgaacttaga agaaaattag ctgaaaagct
     2101 agatttaaaa ccagaaaatt ttattgttgc taatggtgca gataacgtta tcactctaat
     2161 aggtgaggct tttataaata gaggagatga agttatatat tgtaacccaa catttccatc
     2221 atatagaact gcaactataa aaaatgaagg gataccagta gaagtacctt taacagaaga
     2281 ttataagtat gatttacaag gtatattaga taagattaca gataaaacaa aattaatatg
     2341 tgtatgtaac cctaataatc caacaggaac aattgtggat gataaagagt tagaagaatt
     2401 tttaaagaaa gtccctgaaa atattataac tatattggat gaagcatata tagaattttt
     2461 aacagttcct aattatgtgg atggacttaa gtatgtaagg gaaaattata acgttatagt
     2521 aacaagaact ttctctaaaa tatatggtct tgcagggctt agagtaggat atggtattgc
     2581 taaagatgag ataataagaa ctttatttac agtaaaagag cctttctctg caaatcgtgt
     2641 tgctataagt ggagcaacag cagcacttga tgatgaagag tttataaaag aaacatatga
     2701 attaaataga gttggaatgg cttactttaa agaagagttt acaaaaatgg gatttgatgt
     2761 tgtagattca caatcaaatt tcctttatgt agatatgaaa acagatatac ctaagttatt
     2821 tgaagacttg aaaaaaagag ggtttgtaat aagaccaagt gctacacatg cacgtgtaag
     2881 tattggaact atggaagaaa ataagaaatt tgtagcagtt ttaaaggaaa tattaaatat
     2941 taaaggagag ccaagtattg gataatagat agaaatatta tgaaacgggg tgtagatatg
     3001 aacttaaaaa taaacgcaga gagatgtaaa agttgtgaat actgcgttat atcttgtaaa
     3061 aaaggagcat taaaaatatc aagtaagata aacaaagaag gttatgctca tgtagaagtt
     3121 gatgagagta aatgcatact ttgtggaatt tgctatcagg tgtgtccaga taatgttttt
     3181 gaaataatta aagaagaagc atgtcaagag gcttagaagg agttgggata atatgaaaaa
     3241 gatgtggaag gggaaccacg ccattgcaga agcagcccta agaggaggtt gtgaatttta
     3301 tgcaggatat ccaattacac cacaaacaga ggtaatggaa tttttatcac atagaatgtc
     3361 tgaacttgga agaactttta tacagtccga aaatgagatg gctgctatat atatggtata
     3421 tggagcatat gcaagtggaa tgaggtctat gacgagttct tcaggaccag gaatatcttt
     3481 aaaacaagaa gggatttctt acttatgtgc aaatcattac ccttgtgtaa tcgtaaatgt
     3541 acagagatgg gggcctggtc ttggctcatt ggactcagca cagacagact atctaagaga
     3601 tactagaggg ggaggaaatg gagattatca tttgatagtt tatgcaccaa acagtattca
     3661 agagactgta gatttgatgt ataactcatt tgatgtagcg gagaagtaca gagtgcctgt
     3721 cgaaattttg acagaagcag ctttaggtca aatgatggaa ccagtagaat ttccagagtt
     3781 taagaagaga gaaggagatt taggctggac ttatgatgga agtaatagag accatgcaaa
     3841 ggttgcagat aaccaaaagc caactttttg tatggagaaa cgtatgagaa taactgaaaa
     3901 tgaacaacaa tgggaagatt atcaagtaga agatgcagaa tatgtgtttg tagcgttcgg
     3961 tataccatca agaacaacta tgaatgctgt tagaagactt agagaacaag gtgaaaaagt
     4021 tggaataata agaccaataa cagtatggcc tttcccatat aaagcatttg aaaaggtaag
     4081 taaagatgta aaaggattta tctcagtaga atcaacagac acaggtcaat tagtagaaga
     4141 tgttgcttta acctcaaaga aagtatgtaa agaaaatgta cctgtgtatg gtttatttag
     4201 tggaaatcat ataccaaaaa cattgcaagt aatggacact tatagcaaaa taaaatctgg
     4261 cgaaatcaag gaggtgttct aatatggcta cagaaaaatt aacacctagc gttgtttcaa
     4321 taccaaacaa gttttgccca ggttgtggtc atggaatagt aaatcgtata atagcagagg
     4381 taatagaaga acatggatat gaaaaaaatc atgtattaac attaggagtt ggttgtgtct
     4441 gcaatatgaa ttttagctgg aatggtgata agatgcaaac tgcacatggt agagcttcat
     4501 ctacagctat aggagttaaa gtagcacttc cagatacatt agtaatgact taccaaggtg
     4561 atggagatgc ttatgttata ggtctttctg aaactttaaa tacagcatat agaaatcata
     4621 atgtgactgt atttgtaata aacaataata attttgcaat gactggtgga caaatgtcat
     4681 ggaccactat gccgggtcaa gtcacaacaa caagtgtaaa tggaagagat ataaagacta
     4741 ctggtagtcc actgaaagtt cctgagatgg tggcaaactt ctttgatgta gcatatgtag
     4801 caagaggttc agttcatagc ccaaaggaaa taattaatct aaaaaaatat ataaaaaatg
     4861 caatagaagc tcaattaaat ggagagggat attccttagt tgaaatatta gcaccttgtc
     4921 caactaactg gggtgtatca ctagagaaat ctatcaaatg gatggaagaa gaaatagtgc
     4981 catattatgc tcttggcgaa tttaagcaaa gggatggtga atagttatgg aaaaagagct
     5041 agtatttgca gggtttggtg gtcaaggagt tttaactctt ggtctgattg tagctgagtc
     5101 agcacttgaa ttggggaaac aagtaacttg gatgcctgca tatggtccaa ctatgagagg
     5161 tggaaaagcc tatagtgtag taaagttttc tgatgagagt ataggtgggc ctgatatgga
     5221 agaaatagat gttttagtag caatgaataa accatcttta gattatataa atttagttaa
     5281 agaaggtgga acagtagtta ttaatacatc tgcaatagat gagaatgtaa cattaagaga
     5341 agatataagt gtagttaaaa taaattgtca ggaattagct caaaaagtaa acaatccaaa
     5401 agcagcaaat atagtagtac ttggagcatt aattgctaaa acagggttat ttgaaaaaga
     5461 attagcttta aaaactatgt gtgatttctt tgaggaaaaa ggaaaaggaa agtttaatgt
     5521 tcaaaatgaa gcagcattca tggaaggcta taataacgcc atttgataaa ttacccttat
     5581 cttaagtgat ttatctcaag atttaataat ctttagtgcc aagagaaact tcatcttgag
     5641 ataaaattca cttaaaatca caactataaa catataggag cgtgaattta tgagtttgga
     5701 aaaacttatt agaccaaaaa gtattgctat agtaggagtg actgataaac ttggttttgg
     5761 aagaagtgca gcacttagta tagtaaaaag taaggaaaca gatagagtat attatgttaa
     5821 tcccaaaaga gaagaattgt ttgggagaaa atgttataag acgatacaag aagttccaga
     5881 agtagtagac tgtgtagtag tatgtacacc tagaaatgta gttccatctg tattaaaaga
     5941 ttctggagag ttaggaacaa aggtagcagt ggtatatgct agtggatttg cagaggaagg
     6001 tacagaggaa ggtacagatt tagaaaatca acttatagaa atatcaaata catacgatat
     6061 gaaaatattg ggtccaaact gcatggggtt gctaaattgt atagataagg ttaatctatg
     6121 ggcaggaggt tctaaatggg atttagatac taaaaaacct ggtataggaa ttgtaggtca
     6181 aagtggattt ataactgctg aaattgtttc aagtgattat tttaatatat cttatggttt
     6241 ttcaacagga aatggaaata tagttacact agaagatgca gtagatttcc ttgtagatga
     6301 tgattatgca tcagtaatag caatttacct tgaaggttta aaaaatcctc aaaaatttat
     6361 agatgcttta aaaagggcag cacaaaaaag aaagccagta ataatattga aatcaggaag
     6421 gtcagaaaaa ggtgctattt ctgctgcttc tcatacagga aatttagctg gttcgagtaa
     6481 agcttttgaa agtatttttg aaaaatatgg agtaatttct gtggaaaatt tagaacagtt
     6541 tatgtgttta gcacaagctt ttagtgtttt agatggtaat ttacctgcaa attctaattt
     6601 tgcagcaata aacttctcag gaggagaaaa tactatttgt gcagatttag cagaagaaaa
     6661 tggtgtagaa cttgcagaaa tatcttctga aactaaagaa gaaatgaaaa aatatcttcc
     6721 aggatttgca acacctaaaa atccactaga tgctacaaca gcattattcc atgaaaagga
     6781 tatgatagta ggtttattac atacctttga aaaggatgat agtgttggat ttactatggt
     6841 aggtgcaaat ataagagatg aagaaaatga aatgcatgaa accctttgta aagctgtaag
     6901 tgaagctaga gaacaaggat tgaaaaaacc tgtgtttgca gttcctacac ttgaaggaaa
     6961 tagatattta gattatagga ctagattaga agataatcaa gtgcctataa tgtcatcagt
     7021 tggaactaca tttacttgtt ttaataaaat ggctaagttt atagattatg attattcaaa
     7081 aagaacttta gaatttaaag ctgttaagaa aagagagtca aataatgtag tagcattatc
     7141 agaattagat agtaagattg agatgaaaaa atatggagta ccagttccag gacaaggtaa
     7201 tgcaaaaagt attgatgaat tagatgaaat cttaaaatat attaagtatc ctgttgtttt
     7261 aaaaataaat tcttctgaaa tattgcacaa atctgatgtt ggaggagtta aaataggaat
     7321 tgagaataga gatgaggcag tagatgcata taatgagatt ttaactaatg taaaaaaagc
     7381 aaaaccagat gctaatattg atggtatatt agttcaagaa atggtagaaa gtggtataga
     7441 aattattatt ggtataacta atgatgacca atttggacca atgttattag tcggtttagg
     7501 aggagtattt gtagaggtat ttaaagatac tacattatat ccattaccta taaatcatga
     7561 tgaagctatt atgatgctta aaaaactaaa atcatttaag cttttaaatg gctatagagg
     7621 aagtgagcct tgtgatattg atgctcttgc agatatgatg gtcaagttag gaaaatatgc
     7681 ttatgaaaat aaggatgaag tcaaggaaat agacttaaat ccagtgtttg tatatcctaa
     7741 aggaaaaggc gtatgtgcag tagatgcctt aatagttaaa tataaatagt ttataagttt
     7801 tggtattcaa ccataattaa agcaccgact gaaaatcaca ttttgtaagt tagatagtgt
     7861 gattttcagt caccccaaat ttaatttaaa aatattttaa aatgaaagta tagttttaag
     7921 tttaattgtg aaatagcact gtataaacaa taaaatacta cgtataaaac gaataataaa
     7981 aggtgtttta atgataagaa tcaatcttga ttttcattaa aacaccttct ttattatatt
     8041 aaaaattttt aaacgttata ttgtcacagg tgggtttgtt ggagctacat atgtacgatt
     8101 agcaaattct tggtcaacaa agaatagacc accctctcca gcaagtttaa cacgttttat
     8161 attgtcttca caatttgttt cttcttctgc ttgttcagat ataaaccaaa gtaagaattg
     8221 agaagttcta aagtctgatt cagagtttgc tacagctgct aagttgttta ttaaagctgt
     8281 aacctttttt tcatgagcta acgtacgctc aagaatatcc attgcagaag taaagtctga
     8341 atctggcatt gggatagcat caagctgaac ctttcctcct acattatgta agtatttgta
     8401 aaaaaacata gcgtggtctg tctcttcttt atattgaaca taaaaccaat tagaaaatcc
     8461 tttaagtcct tctgcttcaa ggtaagaaga catagaaaga taaagatacg cagaatataa
     8521 ttcatggtta atctgctcat taagtaattt ttccatttta gctgaaatca tttttatacc
     8581 tccaaatttt aaatgctctt ataaaatata ataatattaa atggaaaaat attcaataaa
     8641 agtgtttaaa tattttacat atttaataaa aatttatact accattaaaa aaattataac
     8701 ttacaatggc tatagagcca attaatggtg catttgctcc aaaatttgaa tgccttacac
     8761 ttattttttt atatttagaa aaacttgctg aagcagtcaa tttttttaat aatgtatttt
     8821 ctataaatgt accaggtata ttaaagtcat agcctattat tatagaacta caatcaatca
     8881 atgtaagtgt attaactaat gcatatgata cgtatgtaca aaattcatca agcaaaaaca
     8941 tggcaagact atctttatta tttgcctcat ctattaactt gactaaagaa aaatctttac
     9001 agttaattaa tggggagtta ggataaatat ggctcaatga ttcaattctt ttaattagat
     9061 tactgacact tgtataataa tctaaacatc cattattacc acaactgcac aatggtccac
     9121 taaaattaat tgatgtatgt cctatttctc cactttgtcc taggttgcca gtatgaagtt
     9181 tattttctag aactagacca gctccaatac cattcattat gtgtaggtat atataatttg
     9241 atatattttt acctaaacca tacatttttt ctgctaatgc acccgaatta gcatcattta
     9301 tcaaaaatat tggcaaatca gacatttctt ttataaaatc tactatattt aggtttgata
     9361 catttccaaa atctggggga tttagtatgg taccatttat atcatctact ggacctattg
     9421 aagaaatacc aattccaatt atatctctct tgttgatttt cattatttta gtaaacattc
     9481 tttttattat atcttttaaa acatcatttg atagacattt ggggtaagtt tcagaaagtt
     9541 gttttacaat attacctttt aaatctgaaa taattacctt acatatattt cttcttatta
     9601 ggattccaca aatacaaggc gatatattag aaatatctaa taaaataggt ttacgacctg
     9661 atgaatgtgc atttaaagtt gatgactcta tttccctaat catattttca ttaataagtt
     9721 cagtaactag atttgataaa gtcattttag taagtcctgt agacttagct aaatcaactc
     9781 tggacatagg tgtatttgta gaaagtaatt tcaatattat taacttattt ttaattttta
     9841 catctttgat gttagaacct tgtttgttac tcatcataaa tctccttaaa taaagttaag
     9901 tgttttattt attttcaagt atactatcac cttataatag agtaacattt ttttacttga
     9961 aaatatcatt aattagaata tatcacatta tatatcataa aatatataaa aaaatgttag
    10021 aattaaatta aaagtgaaaa gtagattgat atataaatag taaatactta attaaaaaaa
    10081 gataatgata gcaataatta ttgttaccaa gaatctacta cttcaagtgt taccttaggt
    10141 atttaagttg atagtggttt gcacgtaata aacttgatgt aatatatatt cttaataata
    10201 tcgataaaag atttacttaa aaataatata gattatgtaa acatataatc tatttattgt
    10261 atacaataaa atttcatttt gtaaaacagt gaattaaata tgtaatatag atatcgattt
    10321 ttattacaaa ttgttactaa atataggtac attagaacaa aaatctagta aa
//
LOCUS       DAMKMI010000136        10266 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMKMI010000136 DAMKMI010000000
VERSION     DAMKMI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.142"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..860
                     /locus_tag="PC429_003085"
     CDS             <1..860
                     /locus_tag="PC429_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3848568.1"
                     /translation="YILFSFVIISSLIISLDKNIYLALESSSILLIITVLSIYIENEN
                     LSKLYSQNLYDKLRISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSL
                     STTIIQLNAIEKLLKDKPLIYDLVHELREFVTESFQDVRRAISELKPVEYENYQSLFK
                     IKELVKSFIKLTNINVKLTISKNTWNLSRNQSIALYRLIQESLSNSSRHGKATEIRIF
                     ITFNTSNLIITISDNGIGCGNIKKGNGLNSINERIYELNGKVEFDNSNNGFTIRASFP
                     KFSGGDFFE"
     gene            853..1500
                     /locus_tag="PC429_003086"
     CDS             853..1500
                     /locus_tag="PC429_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848569.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1629..2564
                     /locus_tag="PC429_003087"
     CDS             1629..2564
                     /locus_tag="PC429_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848570.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2578..3675
                     /locus_tag="PC429_003088"
     CDS             2578..3675
                     /locus_tag="PC429_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848571.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3678..4742
                     /locus_tag="PC429_003089"
     CDS             3678..4742
                     /locus_tag="PC429_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848572.1"
                     /translation="MIRLTIANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            4986..5483
                     /locus_tag="PC429_003090"
     CDS             4986..5483
                     /locus_tag="PC429_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF3848573.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5659..5907)
                     /locus_tag="PC429_003091"
     CDS             complement(5659..5907)
                     /locus_tag="PC429_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848574.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(5973..6989)
                     /locus_tag="PC429_003092"
     CDS             complement(5973..6989)
                     /locus_tag="PC429_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF3848575.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGIACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7047..7814)
                     /locus_tag="PC429_003093"
     CDS             complement(7047..7814)
                     /locus_tag="PC429_003093"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF3848576.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7882..10071)
                     /locus_tag="PC429_003094"
     CDS             complement(7882..10071)
                     /locus_tag="PC429_003094"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF3848577.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3568 a         1580 c         1189 g         3929 t
ORIGIN      
        1 agtacatatt gttctctttt gttataatat cttctcttat tattagttta gataaaaata
       61 tatatttggc actggagtca agctctatat tgctaattat aactgtactt tctatatata
      121 tagaaaatga gaatttatca aaattataca gtcaaaatct ttatgataag cttagaattt
      181 ctaaagatga attaaaaaaa gttaatgctg atttggaaac ttatgcaagt tctatagaag
      241 aattagcaat tttaaaagaa agaaatcgaa tatcaagaga aatacatgat agtgtaggac
      301 attctctttc tacaacaata atacaattaa atgctataga aaaactctta aaagacaaac
      361 ctctaattta tgacttagta catgaactta gagaatttgt aactgaaagt tttcaagatg
      421 taagaagggc tatatctgag ttaaaacctg ttgaatatga aaattaccaa agtttattta
      481 aaatcaaaga attagttaaa agctttatta agcttacaaa tattaatgtt aaattaacta
      541 tttcaaaaaa tacttggaac ttatccagaa atcaatctat agctttatat agactcatac
      601 aggaatccct ttccaactca tctaggcatg gaaaggcaac tgaaatcaga atttttatta
      661 cattcaatac ttcaaatctt ataattacta ttagtgataa tggtatagga tgtggcaata
      721 taaaaaaagg aaatggactt aatagtatca atgagagaat atatgaatta aatggtaaag
      781 ttgaatttga taattctaat aatggtttca caattagagc ttcttttcct aaattttcag
      841 ggggtgattt ttttgaataa aattaaagtt cttatagtag atgacgaaaa attaattaga
      901 aaaggtctta aaattatatt atcatcttat aatgatttag aaattgtagg agatgcttct
      961 aatggatatg aagctttaga attttgtaaa atgaatgatg tagacatagt tcttatggat
     1021 atacgaatga aagtatgtga tggggttcta ggtacaaggc ttataaagga atataataat
     1081 tctataacat tattaatttt aacaactttt aatgatgatg aatatataaa agatgctatg
     1141 aagtttggtg cttcaggata ccttttaaaa gatagctctg atgaagtatt gcatgaaggg
     1201 attcgttctt cattttttgg caatattgtg cttgataaaa gtgtagctga aaaaatcatg
     1261 acctctgaaa aaacggttaa acaagagcat ctttatgaca tgtacaattt aacagaaaaa
     1321 gaaatatcta taattagact aattgcaaat ggacttaata ataaagaaat aagtcaagaa
     1381 ctattcttat ctgaaggtac aataaagaat aacataacta atattttagc taaactagaa
     1441 cttcgtgaca gaactcaact agcaatcttt gctttcaaaa ataaaattgt aatagaataa
     1501 atctaaaaat ataaagtgac ttaagtcact ttttttagtg acttatagta atagaattat
     1561 aattcacaat tctttattat aatattatag ctttttaact tatacaaaat tattttggag
     1621 ggattactat ggatatagtc agagtaaata atataacaaa aagatttaat gataaattag
     1681 tacttgataa catcagcttt tctgttaaaa aaggtgagat atttggactt attggcccaa
     1741 atggtgcagg taaatctaca cttataaata taattacaaa tcttatgtta ccaaatagtg
     1801 gtagtattca aatcaatggt ttagatttat caaaagatta tataaaagca aagtctataa
     1861 tagggttagt tccacaagaa ttagcaatta tagaaactct tactcctttt gataacttag
     1921 agtactttgg agctttctac ggtttaaagg gaaaactact taaagaaaga atcattgaag
     1981 cattagaagt tacaggtctt actgaagtca aaaagaaaaa ggtcaaaaaa ttatctggtg
     2041 gtatgcaaag gagattaaat attggaatag cactcttaaa tcatcctaaa atattaatat
     2101 tggatgaacc aactgtaggt gtagaccccc aatcaagaaa tcatattttc aattttataa
     2161 aagatatatc taaaaaacat gaaactactg taatatatac ttcccattat atggaagaag
     2221 ttgaacacct ttgttctaaa atttttatta tggatgaagg taaagaaatt gcttttggag
     2281 ataatgatta tttaaaatca ctcgtatcta caaatacaaa attgattatg gaaataaaaa
     2341 atataaatgc ccaacttatc tttgatttaa aaaatactaa aggtgttata tctgttttag
     2401 aaaataattc attattagaa ttggatatag ataaaaaatt acaacttaca gatatactct
     2461 ctgttattga caaaaacaat tcaaaaataa tgaaaatttc ttatgaagaa cctagcctag
     2521 aagacgtatt tttaaatcta acaggaaaaa atttaaggga ctaatggagg tgattttatg
     2581 aaattataca tgagtgtaaa aactatgcta aagagtttaa aaagctcctt tatacttaat
     2641 ttaatatatt ttttagcttt gcctctgatt ttatcttggt ttcttgggat ggttacagaa
     2701 agcatgtttc aaaaccctat aaaaacagaa tctaccccca ttgttatata tgacaaagat
     2761 aacacaagat tatcaaatga tttgacaaag tatttgaaaa atgatttatc ttatatatta
     2821 acagttaaaa aagatgattc taaggcagaa ttaaagctta ctatacctaa aggctatgag
     2881 agtagtttat taaatgaaaa atcaaatact ctaaacatag aaaaacttgg aactcgtgat
     2941 gatattgcaa tattgcttca agatatatta gatacttacc atgaaaaatt ttatttaaat
     3001 aattctcaga aaataagttc agaagatttt tctaaattat ttaataaaaa ttctattgat
     3061 acttctataa taaataacaa tgttaaacag agctcttatg aatactttgc tttagtttca
     3121 ctaggatttc ttgtaataat ttttataatg aacaatattt tatcaaatta tataagtgaa
     3181 tcaaagggac tttcaaaaag actttattct atgcctataa ctagagtaca gttcttaatt
     3241 tatgactttg taggtttatg gatttattca tttatattct tattattata tgttttattc
     3301 tttagaatac ttggaataac atttaaaggt aattttgcca tattgctcct actttgtgct
     3361 ttatcatctt attttatgac ttcaatatct acctttgtaa ccagcttttt tagtaaaaaa
     3421 tacggtacag caatagttta tgcattactc tttttacaga caatttttgg aggtatcttc
     3481 agtatgataa gtgatacatt tactaagctt acaagtttat ctcctaccta tttaatagga
     3541 gaattgttta gtaattatga aacatttaaa actatagatt caataggaga tttaattttc
     3601 acatgtttaa ttatctctac aatattaatt gcacttgcta ttgtaaagga aaaatataaa
     3661 tggagggagg tttaataatg attagattaa ctattgcaaa tataaaaaga tatctaaaaa
     3721 atcatactct tttaattaat atggttatgc ttcctattat actaattttt tcacttaatt
     3781 tttttatcaa caactcagga aatcaatcaa tgtacttttc tccagtagca attgtatctg
     3841 actcttcagg aaagtatgaa cataagttaa taaatagttc taaattaaaa gaaaattcat
     3901 ttagattaaa tgaacaagat aaggctatga atctattaaa aaataataag gtttctgctg
     3961 tattcgtttt agataaaaat ttttcaaata gtatagataa attacaacga cctgtagtta
     4021 aatgctttaa aattgaaaat ggtggtggta gtctttgggc agaatcacaa atagaatctt
     4081 ttattactaa atcgttaaaa ttgaaagtgg ataaaaatat agatgataaa ttgacaaaga
     4141 caaatataat tgataacaaa ccagataaga ataaaggttc atttttagta gtcttcttga
     4201 tatgctattt tatgtatatt aatgcggcac atcttgcttc tgatttattt acattaaaaa
     4261 aatcaaatgt gctaaaaaga cttatctcta cagataataa agatataaaa ataattttca
     4321 gtatattttt aggactattt tttattcaat caattgtata cacttttgct ctattatgtt
     4381 tttcaattat agaagacttt aatttgagtc tgaatgtatt gatgattgta ctagcaaata
     4441 gttttgtttc cactggattt gtattttggg tagctagagt atttaaaaat gaaagttcta
     4501 taagtcttat atctacattt tattctttaa taggtctagc tatatctatc tctagtctaa
     4561 taccatcaat ggataaatta tcctttatga ctaatctttc aaagttcaca ccattttatt
     4621 ggactataga tgctattaaa aacaatggta gtattttaat aaatataata gctctaatat
     4681 taattggaat tatatttgta actgcaggaa gtcttaaact aagagatttt gctaaaaact
     4741 aataattatt aaatataatt agtatctatt tttatataat tggaaaagaa tatttcaaca
     4801 tattaaatta ttttatctta tatttttctc tttagtttca cacatcacca agtctaaact
     4861 ttatattata tattaatatt tttgacttgt caagttattt atgttataat attaatcatt
     4921 agataattaa agcattgtac ctaagttgca atatctaaaa ttcaatttgg aggtataact
     4981 agcatatgta taaaattggt gaaagtgtaa tgtatccaaa agagggagcc tgctctgtaa
     5041 atgatattgt aacaaagaaa ataaatcatg agatgcaaaa atactatgaa ttaagtgtca
     5101 tttttaatag taaccttaaa atttcgattc cagttctaaa tgcagataga attggcatac
     5161 gtccagttat ggatggaaat gatgttgata attttatcca atctattaat aaaacagatg
     5221 gtgtatggat ttttgataga aaagaaagac ttaaactgta ccaagataaa tttcattcag
     5281 gagatgtttt tgaaattgta aaattaatca aaatgcttat gatacaagac agttcaaaac
     5341 aattgtgtag cacagataag gagtttttaa ataaagctca aaaatttgct ttatcagaat
     5401 tagctgctgc tcaatgtaaa tcctatacaa tggtattaga ggaaatgaaa aaacatatat
     5461 taaattcaaa gaatacaaat taaaccagtt ttttaaactg gttttatttt ttatactaat
     5521 catccatgct actttagctt attttaaact gcctaatcta atattaataa agttatctca
     5581 attagataga tgtattttat tcaaataaaa aaaactaaga gtaaatcata ttactgattt
     5641 actcttagct aataaaattt aatcttcttc aagttctttt ttctcttgta ataatacact
     5701 taattcattt gcttctaaaa cttgtgcctc tgcaatatca agtaactctt gttgccattc
     5761 cacacattta tctttgtaat caaaaggtag cgttctgtca actatgtact tatcaataca
     5821 gtcctccgca cttttaatta tttcttttct ttcatcaata aatttttcta attctactaa
     5881 ggcttcttca taagcttttc tactcatata aattccccct ttgtacaatt attctacatt
     5941 aaaatgattt ttcctctttt ttgtataaaa ttttatttgt atataacttt tccatcaata
     6001 tcttttatag tttgacatcc agttaatatc atagaactac ttagctctga ttgaagttta
     6061 tttacataag tttctacacc atcagttgca ccaccaaaag aagcagtcac aaaaggtctt
     6121 cctattaata ctgcatctgc acctaaacct atcattttta gtacgtctaa accagttctc
     6181 actccaccat ctgcaagtat tgtaacttta cctttaacag catcagcaat ttttggtaaa
     6241 acttcacatg ctcctggtgt acagtcaagc actcttcctc catgatttga tacaactatt
     6301 gcatccacac ctgcttctac tgccattaaa gcatcttcaa cagtcattat acctttcaat
     6361 ataaatggta gtttagtaga ctttactaac tcctttattt gtttaacatt tttaggaagt
     6421 actggttttc catgtaatga taaagttact agaccacagg catctatatc aactccaact
     6481 gcaaatgcac cagcttcttc agctaatctt atctttttta taatattttc attatcccaa
     6541 ggtttaataa atacaattcc agctccatta tactttttga ctacatctaa attttccatt
     6601 aaaaaagcat ctactgccgt atcaccaacc attgcataaa taccactatt agcacatcct
     6661 gcaactactg gttctatgta atctctttca ttaatttttc cacccatatt taaagtagtt
     6721 cctgttactg gagctgcaaa tataggtgca ctcattttct ttccaaacat ctctatagat
     6781 gtatcaggat ttgatacatc atgtattact ctcatattta cttttacttt ttctaaactc
     6841 ttgctatttt ctataaaaga agaaccactt cctttacctc ccatgccagg cacttcacct
     6901 gcacaagcaa tcccattaca aaccttacaa actttacaac ttccatttaa attttctcta
     6961 gcacttttta ataattcatt atattccatc tttaatcccc cattaactat agtaatttat
     7021 attatatata ttatatcata ttatatttac agtatatttt tcaaaaaact ttttctgtgg
     7081 ataggagtta tgccatattt ttcaatagct tcatagtgtt ctttagttcc atatcctttg
     7141 tgagatttaa acccatattc tgggtatact ctatcatatt ggtacattat actatcccta
     7201 gtaactttag caagtatact tgctgctgct attgatatag attttgaatc tcctttcaca
     7261 attggatttt gagatatgtc aatacctggt attgttgctg catcaactaa taaataatct
     7321 ggtgcttttt ttaaacaatt tatagccttt ttcattgcca tatatgtagc atttaatata
     7381 ttaaattcat ctatttcttc attatttact atacctattc cataatctag tgcttcttct
     7441 tttattactt caaaaagttc atctcttttt gcctcactca gtttcttaga gtcatttaca
     7501 ccttctattt tggtatcttt tttaaatact acaacagaag ctacaacagg tccagcaagt
     7561 ggccctcttc ccgcctcatc aattcctcca atatataaat aacctttatc atagccttca
     7621 ttctcaaata tatttatagt ttccaatctt tcttcttctt ttctaatatt atccagcttt
     7681 tttgctagtt ttatagctag accttgtaca gattttcttt catcaactct taacagctct
     7741 atatattcca tatatttttc tacttctaaa gtctctatta tttctttaat ctccctcaca
     7801 cttttatcct gcataccatt ccctcatctt tcgtttttaa aaatttaatt ttttaatata
     7861 ttaaaatttt ttaattagag tttaattgca tccactatag ccacatttta aacatatact
     7921 acatccacct gtatgtgcaa ttccttcact tccacactca gggcacacaa atttttcatc
     7981 ttcttcaaaa tttttattta ttatttcttt ggctttttca tctttattat ctttttcttt
     8041 tttattattt ttataatatt cttttttact attttctttt ttatcttctt tattattttc
     8101 ttcctttttt tcatttatta aaattccact ccttttacat ccatctctaa atattgttac
     8161 tcctttaaga ccagcctccc acgccttaat atatatattg aaaacatctt caactgtagt
     8221 ttcaaaaggt atatttattg tagatgatat agaagcatca atatactttt gccatacttt
     8281 ttgcatgttt attctttctt catatcctat attcatagca gtaacaaaaa aatctggtaa
     8341 acattcttca tctgtaatac ctttcttgtc catgtactct ttaacaattg gagtatatac
     8401 tttataatac acatcctcat catgcaagct ttcagttttt cttatatatg aaagattaaa
     8461 tataggttct attccaccac tgatacctat cattgtagat atggaacctg taggaggtat
     8521 ggttaaaagt tgtgaatttc taagcccata ctttttaacc atttccttag tgtcttcatt
     8581 tagattttct ataaaaaact tagatttaga aatattttca tatttatact cactaaatgt
     8641 tccatattct ttagcaatta atgctgattg cttcacagct tcatttaaca tagtcttacc
     8701 aattaaatca catagttcta aagattcatc agaaccatat cttattccca tttttataag
     8761 catatcagct atacccatta cacctaaacc tatctgtcta tacttatgta cactatctct
     8821 ttgttcttgt aatggatgca agttcaatcc ctcatctaag acatcattta aagctactac
     8881 agcttctcta acgcaagatt ttaatttatt aatatcaaaa gtagcatgtt caccaaatgg
     8941 ttgtattata aactctgaca agtttattga gccaagtaga caacttcctc cacttggaag
     9001 tggttcttct gcacaaggat ttactcctga aaagcaaaag ttcttatctt cactcataaa
     9061 attatatgtt tttattttat cccaaaacaa tatacctggt tctgcataat cccagttatt
     9121 tttcacaaat ttattaaata aatcataagc atttactatc ttaactattt tttctccagt
     9181 agtttcaact tcaaaagtac aattatataa ctttttttct cttacagctt gcataaattc
     9241 atctgttact cttacagaaa tatttgcttt agtaaccttc tccaaatctg tttttatatc
     9301 tataaagtct tctatctctg gatgatttac atccatagaa atcataagag ctcctcttct
     9361 ccctctttga ccaattaagc cagttgtcat actgtaaagt tccataaaag agactgctcc
     9421 agtcgtatgt tttgcagcat tgttaacttt cgctcctttt ggtcttagct ttgaaatatc
     9481 aattccaact cctccaccat aactaaaagt tctagcaagt ttttttgcag tatcaaatat
     9541 atcttctatg ctatcttttg gtggttctat tacataacaa ttagaatatg ttactttttt
     9601 tccctctttc tgtaatcctc tatttgctaa aattcttcca gcaaatagaa atcttttttg
     9661 tcttattagt tttgatattc ttgaactatt atttgaaatt ctctttatcc attcatcaaa
     9721 actctcacca ttatgtctat acttgttctt ccatattgat ttttgtaatt catcaaactc
     9781 ccaagcttta gtccttattt cagagcgtct ttctctatat aatatgtagt gttttgctat
     9841 atcttttcta tttgattcca taagtagtac ttcaactata tcttgtattt cttctacgga
     9901 taagcatttt tccatagaat tatagtcatt agctattttt cttgatatat catatgccaa
     9961 ctctctatct actccacctt cactatttat actagcttta tagacagcat cttcaatctt
    10021 acttttacta aaaagttctt ttgtaccatc tctttttatt atgtatttca tttttaaacc
    10081 tccaataaat tagtattaaa ttaatacaca cattaactat aactctaaac aaacttttta
    10141 tatatttgta tattttcata ttaattatct aacataatat atattatact attttatatt
    10201 caatataaca ctccttatag ttttcttaac ttgtaaaata tcttcctaaa aaactcaaaa
    10261 ataata
//
LOCUS       DAMKMI010000137        10258 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMKMI010000137 DAMKMI010000000
VERSION     DAMKMI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10258
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.143"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            187..966
                     /locus_tag="PC429_003095"
     CDS             187..966
                     /locus_tag="PC429_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF3848578.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
     gene            complement(1276..2097)
                     /locus_tag="PC429_003096"
     CDS             complement(1276..2097)
                     /locus_tag="PC429_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF3848579.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGNVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDADVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEIWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(2772..3590)
                     /locus_tag="PC429_003097"
     CDS             complement(2772..3590)
                     /locus_tag="PC429_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3848580.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            3758..4339
                     /locus_tag="PC429_003098"
     CDS             3758..4339
                     /locus_tag="PC429_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3848581.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            4336..4530
                     /locus_tag="PC429_003099"
     CDS             4336..4530
                     /locus_tag="PC429_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848582.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(4694..6436)
                     /locus_tag="PC429_003100"
     CDS             complement(4694..6436)
                     /locus_tag="PC429_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF3848583.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            6865..8241
                     /locus_tag="PC429_003101"
     CDS             6865..8241
                     /locus_tag="PC429_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF3848584.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            8528..9199
                     /locus_tag="PC429_003102"
     CDS             8528..9199
                     /locus_tag="PC429_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF3848585.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            9388..10185
                     /locus_tag="PC429_003103"
     CDS             9388..10185
                     /locus_tag="PC429_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF3848586.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRIVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDVNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
BASE COUNT         3784 a         1244 c         1568 g         3662 t
ORIGIN      
        1 ttaaatataa tcaataccga ttctataata ttaacactat tttttatgct atataacaat
       61 tattgaaaat gatataaata taatatctca aataatatga atataacatc tgaatagagg
      121 gtgttttatt tgtaaaaaat aagtttacag tttgagcgta taaacttatg gtaaaggaga
      181 ataattatga gttataagaa gattgcaatt ataacgggtg ctaatagtgg ttttggtaaa
      241 gagtttctaa agttattaat aaatgaagaa gaaataacag aaatatgggc agttgcaaga
      301 aacaaggcga gattaaatca attagtagat gaatttggaa gtaaggtaaa aattttttca
      361 aaagatttat caaaaataga agaagtaaaa gaaattggta catttttaag taaagaaaat
      421 gtatgtataa aataccttat aaacaatgct ggttttgcaa aattctgttc atacaatgat
      481 ttaagtatag atgaatctat taatatgatt gatttaaata ttagtgctgt tgtagcattg
      541 ggacttatat gtattccata tatgcaaaag ggaagccata tcatgaatat ttcttcacaa
      601 gcatcattcc agccattgcc atatcaaaat atttatagtt caactaaagc gtttataaga
      661 aattatacaa gagcattaaa tgttgaactt aagagtaaag ggataaatgc aattgctgta
      721 tgtccaggtt ggatgagtac aaatctattt aagagaggta tagttgtagc tgaaaaaggg
      781 acaaaaaatt tcccaggcat agtgtctcca gacattgttg ccaaaaaagc attgaaggat
      841 gcaaagaaaa ataaggatat ttctgtgtat ggtataaata caaaatccag tcatttatta
      901 gcaaaactat tgccacaaaa aatgatgatg aaggtttggt taatgcaaca acatattaag
      961 gaataagagt ttcggtaatt aagtatcagt tggagattca atctgttata tattatgaga
     1021 agttttgttg agcaaataat aattgtgaca aaacggctta tctagtgccg ataagccgtc
     1081 tataatgtat ataaaatata tttttgattt tactttattt agatattgaa gtattaaaat
     1141 atgtaggact tgaaatttta taaagataaa tactatgata ttttgtaaaa actgagatat
     1201 gttgatatta catagtttaa ctatactgtt aaattgaagt tttcttattt tgcattatgc
     1261 ttattactta atgatttaga ctataatttc cataccatca tatgcaaaat aaatgttatc
     1321 aagtttcttt tctaattcac tgtaataacc atatgatttt ccccatattt cgtcaatatg
     1381 tgtaatgata atctttttaa ttctataata gtttttaatt tcaacaatct catctaaagt
     1441 aaatatttca tctttaaatg gtgtgtcttt aagttttgta ccatcattta atataccatc
     1501 atcagaaact agacttatta ttaaaacatc agcatcaaag tacatatcac tatgagtaaa
     1561 tggcttggga ttacagcagg catatatgac ttttttttca ttctctttga taacatagaa
     1621 ggttatattc ctgtctgggt tattgttaat caaatcaata tcaaaattat ttatctttac
     1681 atgcgaagtt tcattaatag aaatgcaatt tataacatta ccataatatt taagacaatc
     1741 gagattcgtt cggtttatat cttcaattac ttcaggaagc gcataaaagc caattggatt
     1801 attttctcct gtgataaagt cacatcctat tttttcgaca attctaatac ccttaacatg
     1861 gtctgggtca tggtgagaaa ttgaaatatg ttttacttcc gatatttttt gtctgttaca
     1921 agctactgca atatcttcag gtgtgtctat cagcattttt atatcatcaa tatataaact
     1981 tggccctaat cgttcatatc ttccgccttt ttgtctggct tcttcacata ttttgcattt
     2041 gcaaaatgaa cttggaataa ttgacatacc gccactacca acaattttta atttcaaagt
     2101 tattacacca ccttcttaaa atattagatt atataaataa tctacaatca caactgatta
     2161 acaaaattat gagttaataa attatatcac atgtaaatat gaaagaatag tatgaaattt
     2221 ttataaacat atattaaatg tttctcaatg aaattgactt tgtatatgaa ctgaatgact
     2281 tattcaatat ggataagaca tttttcttgt aataagatat gattgtccta taaggtttat
     2341 gtaattttga tataatttga tataatatat tgattaacaa catacatgtt attggtgagc
     2401 atatttctat agaatggaat atataatttt ttatatttat cattgtatta ttgtctaaag
     2461 aaagttaaaa agagtagagc acaaaggaat tatttgtact ttactctttt attgttttgt
     2521 taaaaaaact aatttatatt agtgattaaa atatatttaa ctagagtata aggatagtat
     2581 ataatcttat aattgattta tctgacaaat taagataata tcctaattaa attgtagata
     2641 tttatacata acactaaaag taatttgttt aacaaattaa gataatatcc taatcaaatt
     2701 gtaggtattt atacataaca ctaaaagtaa tttgtctaac aaattaaaat attatccaat
     2761 taatataaaa attacttctt tttaactggg aagcatacct ctgttaccca attatcagga
     2821 ttactatcgt tgccagggct tacatgataa atattaaaca taggaccatc cacatcatag
     2881 ttattatctg atatccaatt accaatagcc tcacaagcgt ctgcaacttg attgaaattt
     2941 ccattaacaa ttgcagttgc agcagtcaca cttggaacag tcttaaattt aacatgttca
     3001 gtatctttat actttccaga tacagcaact tgtacttcaa catctacata atcttcttta
     3061 tatccagtat cataaaaaac tgctttagaa tactttgggg gttcaatttg aacattctgg
     3121 tcttttgtct ctaaaaatac ttgatgccat aacattcctt cgtcttggta agttggtata
     3181 acccttctaa gtgtcatcat atattttggt gcaaaattct ttatagttac atcataattt
     3241 ttcataatat catttcctcc aattctttta atagtggttt ctatttttaa tagttttttc
     3301 tgtgtatctt ctaattgttc ttttacttga gaatggtgga tatttagata ttttatgaga
     3361 ctttctttat cgttatattc tgtcaaaatt tcttttatag aatacaagcc aaagcccata
     3421 tcttttagtg catggattct gtttgtagta gaaagttggt ctgcactata gtatctatat
     3481 ccattagttt tattcgtatg agaaggagtt aaaagtccta tttcatcata atgacgtaac
     3541 atacgaatac taattttact tagctttgaa aaatcgccta tcttaaacat gtttttgtct
     3601 cctttataaa ggtactttta gcttaatatt aatataaacc atatcataat gttagagtca
     3661 actaaaatta taattaaatt atattgacat atgatgaaaa aaatattata ctgaacatgt
     3721 tcagaaccga aaatgttaat tgttttggag gtctattatg aaaagtaatg ataaagatgc
     3781 tagagaacgt ataattgaag ttacattgaa tcttttaaat gaagtagatg atattgagga
     3841 gattacagtt agaaaaattg ctgagcgtgc aaatgttgga gttggtttaa taaattatca
     3901 ttttaaaaca aaggataatt tgttgagtac tgccatagga gatgtgatgt caaatataat
     3961 agcagagcta tatgacgata gtgtatatac tctaagacct attgaagatt taaaaaattt
     4021 attaaaaaaa ttgtgtgata ctggattaca ttatgaaaaa gtgcttcctt ttgtattgaa
     4081 tcaatgtata accaatggag atatgcaagc agaacttgat attgtcccta tgcttcgtaa
     4141 aatttttgga aataaaaaag atgaaatgag tctaagaatc attgccctgc aaattatttt
     4201 accaatacag atttctgctt tatctacaga atcatttcag ttatacagtg gtataaatat
     4261 aaagaataaa tatgagcgag ataaattcat tgatatttta atagaaaata ttattggaga
     4321 ggatgttgat gtaagatgaa aattataact attggttcaa gtcttatcac tgttttactt
     4381 tttctttcaa ctatggtttg tgggttctgg attaagaata ataaggtaac agatgcaagt
     4441 tctattaagt tccatatgaa tagtgctatt tttacaggga tatttttatt gatttccaca
     4501 atccttttaa ttatatatat aaaaaaataa taatattatt aataaataaa gtatcagaaa
     4561 ttataaagac aagagtctta aaaatttctg atactttacc atataatttt attaaacatt
     4621 aattgtgtgt gaaaaaatat cacttatttc aatctaatac taaatcttaa gagtgagtat
     4681 aaagtattag aaattaagaa gcatttttta gttcaagtgg attatctata ccaaaataat
     4741 catataaagt agagcttatt tcctctaaat ttccattttg gaaaggtaat tgtaaattta
     4801 ccttttctac cacatcttta aatccagcat cagtattttg atttaaaaaa tccatatata
     4861 catcaacagc ttttctgtaa tcttcttgtg ataattgcca tatctgaagt gatacatcag
     4921 atgcaagacc ataactcaag taataaaaag gaacttggaa gttatgagat acaagtatcc
     4981 aaaaaggagc atttttttct ctaagtatat tattggaaac tccatattct tcctctaaat
     5041 caaaaaacaa tttattgata tcatttagac tcatatatgg attcttgtat ataatttctt
     5101 gaaattcatc ataaagacaa gcatcaataa tagtatttaa aacaatcgat aagtgttctt
     5161 tttttatagc ttcagattgt ttaccaaaaa aatcatcaaa atatttataa aatagaattt
     5221 ctaaagatgt tgaatgaact tcacatacat ctatacttgg ttgcatctta ttatttaagt
     5281 taatactgtt gtagtaatta taaaaatgac caaactcatg agcaaaacta gtaacaccta
     5341 aaaaagaatt gtcccatgta ttaaacaaga aaggtgcttt atatttattt atataagtag
     5401 tatatcctcc aggtgatttg ttttttccag aagaataatc gtacaagtca tattttttca
     5461 tatagtcgaa gctctctttt agttttggag atatatcttc aagtacttta tcaaacttcc
     5521 taaatgagcg atttttatat actttaatat aaatagaaga atcagatggt ttagatgaaa
     5581 tctctctata taatggaact atgtaatctt ttacatcttg tctaaatttt ttagcttctt
     5641 cttgagaata gtctttgttt aggttcatat aggcataatc tatataattc ttaaatcctt
     5701 gcttacttgc tatttcagtt cttgtctgta taagttctaa gaatatgtta ccaaatatag
     5761 gattgtattt ttttaagtaa tctgaataaa ttttcacata ttcttcaggg ctaagatttg
     5821 gtcttgacaa ggcttcttca aagtctacct ctataccatc tattgatact gtagatttag
     5881 ctaataaatc ttcatatttt acaactaggt cttgctcttt tttaagcaaa ccttcaactt
     5941 ctttagaatt aagttttcca atgttcttta gataattaaa tgtatccttt ccaagttctt
     6001 tttctaattc tttagagaat ttagaagtta caaatctatc ttgaaagtct ttgtatgaca
     6061 tgtcaacatc tactgattta ctcataagat acttatattc cttagagtaa aattcgtctg
     6121 atgaatctat gttatttcta atttttgcta tagtaagtgt actgtaaaaa tcctgataaa
     6181 ttttatcaac ttcattaaat aaatttaatt gttccttagc tgttttagct gttaaaagtt
     6241 tgtcgttaca cttgttaatg ttttcgcaaa ttgactgtat attaggacgc ttatattcca
     6301 ttttttcaaa aggaatagtt cctctaacag aatctttttt tgtatcatta gtataatttc
     6361 ttatgtatga tgcaaatata ataaaaactg caattaaaat tagaaaaaca aatattttct
     6421 tcttggattt aaccataaaa atcctccaat catggatata gttattaatt attttactat
     6481 attaaatata aaaaatgcat gttaagaaaa tttaacataa agatatagat aagattataa
     6541 ataatataaa aaatttaact tcaaaaaagt atatgaaatt tatactggta aaatgataaa
     6601 taaattttaa gtacaaaata gtaaaagcta tgatagtttt agacctatta tatgtaaaaa
     6661 atatagatgt ttttggtgca atttaaatta aaagatatat ataaaaattt aaaatattcc
     6721 aaacagaaat tataaaaatt actatcattg tctttataca atatttatac tataatagaa
     6781 taataattta gaggaaagct ctaaaaataa acaaattaag ggtcttaaca gtagtgattt
     6841 tatgttaagg aggtataaag aaagttgaaa acaatagtta gaagacttat tctctacgca
     6901 gatggaatgc gtcctactca aataatggta tctgggtttg ctgcgattat tgtaatcgga
     6961 gcattgttgt taacactccc gatagcgtcg cagagtggag agagtatagg cttattaaat
     7021 gctctattta cggctacttc agcggtttgt gttactggtc tagttatggt agatacagca
     7081 acttattgga gtttatttgg acagatagtt ataataacat tgatacaaat tggtggtctt
     7141 ggatttatga ccgtagccac aatgttttca cttatggcac gaaaaaaaat acaactaaga
     7201 gaaagattat taatacaaga gtcattaaat caagctgatt tatcgggact tgtaagactt
     7261 acaagatttg tattaataat aaccataaca atagagggaa taggtgcact ggttttatca
     7321 actgtcttta ttccacaatt tggtttgtct aaagggatat ggtatagtgt atttcacgca
     7381 atatcagcgt tttgtaatgc cggatttgac ttgatgggca gtgtaagtgg cccatttaca
     7441 tcgttaaatt cttatgtaaa caactttaca gtatcaatga ctgtttgcgc acttatagtg
     7501 cttggagggc taggattccc tgttgtactt gatattgtaa gaaaaagaag attttcaaaa
     7561 ttaaatgtgc attctaaggt tgtattgttt tcaactgcta cacttatttt tgtgggtgca
     7621 ttatttattt ttcttataga atttaatcaa aaagctacta tggcggactt gcctttaaag
     7681 ggaaaagtac tatcagcaat atttcaatca gtaacagcaa gaactgctgg ttttaatacg
     7741 cttgatttag ctactttacg agaaagtagt gtatttgtaa tgataatttt gatgtttatt
     7801 ggagcatcac ctgcatcaac tggtgggggt ataaaaacaa caacactagc tgttttaatc
     7861 attacagtta gaagttttct atcaggaaaa tcagatatag aagcttttga aagaagatta
     7921 gctccatcaa ctataaaaaa atctcttggt atatttgtaa tcagtatatc agcagttatt
     7981 tttggaacat tgattatttc aataactcag cctaatttta cattagttca atcagctttt
     8041 gaggttacat cagcacttgc tacagttggt tcaagtttag ctggaactcc aaacttaaat
     8101 gctttaggta agattataat aataatcttt atgtttatgg gtagagttgg ttctcttact
     8161 ttatttatgg ctatactttc tggtggtaga agaaagagtc aaccaatcag atatgcagaa
     8221 ggtaaaatta tggttggtta agttaccaag gagtaatata aatttataat ggatttagag
     8281 tagcactgta aatctgttga taaaataaat gagcctagat gtaattatat gaagaaataa
     8341 aatcgactaa cgaacaaaat taattaatga aataaaaaaa cagggaaata aaatacaaat
     8401 aaaaaggtaa atcaaaatta aattaatctg tgtatataaa attatatatt gtgtataagt
     8461 tttgtatgtg taaacaatta atttaagaga tattatataa attgtgtatt agggaggaag
     8521 tttcgagatg aagcaatata tagtcattgg gtgtgggaga tttggaagtt cagttgcgtc
     8581 tactatgcat cttttaggac atcaagtaat ggcaatagac aaaaatgaag attcagttca
     8641 aagtatatct gacaaggtaa cccattcact tatagtggat gttactgatg agcaagcgtt
     8701 aaggtcatta ggtttaggta actttgatgt agcagtagtt gcaataggtt ctgatataag
     8761 ggcatctata atggcgactc ttatagccaa agaaatgggt gtagagttga taatatgtaa
     8821 ggcaaaggat gaattacaag ctaaagtgct ttataaaatt ggcgcagata gagttgtatt
     8881 tccagaaaga gatatgggag taagagttgc acacaattta gtttcagata atatattaga
     8941 ccatattgaa cttgacccag agtattcaat tgttgaaatc gtaactccaa atagttgggt
     9001 tggcaagaca cttatagagc ttgaattaag agctagatat gagataactg tacttgctat
     9061 aaaaacaggt aaaaatataa atgttacacc ttctccagat gaggaactta cagccggaag
     9121 tatcctagtt ataatcggtc aaaatactag tataacagcg ataacatctg gaaataaggg
     9181 gataattaga agaagataat ttactattta atatatattt aattgcaatg aaagtagata
     9241 gtatcatata attatgaata gttatatgat actatttttt taatcgaagg tagtatttat
     9301 ttgtaagatt agataaagag aaattaaatt aaaagtaagg aggctgtgct aatataaaaa
     9361 tttataagtt attagcattt agataaaatg attacaaata taaacagtaa ggataatgaa
     9421 aagttaaagt atacaagagc actattaaaa tcaaagaata ggaataaaga gtcaaagttc
     9481 ataatagagg gatacagaat agtaatgctt gcacttgaat gtatggcaaa ccttgattat
     9541 gtatttatca atgaagaatt tgaaaataag aaagaacatg taaaactatt agaagatttg
     9601 gataaaaaaa acacaaagat atacaagact actaataaaa actttaaaga attagtggat
     9661 acagaaaata ctcaaggaat aataggtgta gtttcattta agaaaaaaaa attaagtgaa
     9721 agtataaata aaaaagataa atttgtattg attttagaca gaatacaaga cccaggaaat
     9781 atggggacta taataaggac tgctgattct gctggagtag atgccataat agcactaaag
     9841 ggatgtgtcg atatatacaa cccaaaagta attaggtcta ctatgggttc tatttttgat
     9901 gtgaatataa ttgatgcttc acaagatgaa actgtggaca tgcttaaatc attggatttt
     9961 aatatagttt caagttactt aaatacagaa aatttttatg acaaaataga ttatggctca
    10021 aaagtagcat tggtgatagg aaacgaagca aatggaataa atgaagaact tgtatcaaag
    10081 tctgatattt tggttaagat acctatatat ggtaaagccg agtcgttaaa tgctgcgata
    10141 agctctgcta tactgatgta tgaaataaaa aaatacttaa tttaatgtat tgtaataaat
    10201 ataatgtcat gttataatct tgaattaaat agtagagtat caaaaatagt caatatat
//
LOCUS       DAMKMI010000138        10176 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMKMI010000138 DAMKMI010000000
VERSION     DAMKMI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10176
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.144"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(58..657)
                     /locus_tag="PC429_003104"
     CDS             complement(58..657)
                     /locus_tag="PC429_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF3848587.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(675..3794)
                     /locus_tag="PC429_003105"
     CDS             complement(675..3794)
                     /locus_tag="PC429_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF3848588.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            4015..4839
                     /gene="pdaA"
                     /locus_tag="PC429_003106"
     CDS             4015..4839
                     /gene="pdaA"
                     /locus_tag="PC429_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF3848589.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            complement(4936..5721)
                     /locus_tag="PC429_003107"
     CDS             complement(4936..5721)
                     /locus_tag="PC429_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848590.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            6186..6551
                     /locus_tag="PC429_003108"
     CDS             6186..6551
                     /locus_tag="PC429_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848591.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            6629..7183
                     /locus_tag="PC429_003109"
     CDS             6629..7183
                     /locus_tag="PC429_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF3848592.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            7403..8077
                     /locus_tag="PC429_003110"
     CDS             7403..8077
                     /locus_tag="PC429_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF3848593.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     regulatory      8432..8528
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8582..8758
                     /locus_tag="PC429_003111"
     CDS             8582..8758
                     /locus_tag="PC429_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848594.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            complement(8946..9185)
                     /locus_tag="PC429_003112"
     CDS             complement(8946..9185)
                     /locus_tag="PC429_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848595.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(9319..10128)
                     /locus_tag="PC429_003113"
     CDS             complement(9319..10128)
                     /locus_tag="PC429_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3848596.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
BASE COUNT         3513 a         1577 c         1012 g         4074 t
ORIGIN      
        1 aaagtaggaa tttagatata ctcttatacc atgtaattat gtataaatat ttttttatta
       61 tacaccaata gcttttttag ctaatttatc tgcttcctca ttatacttat ttccagaatg
      121 agctttaacc tttgtaaatt ttacattaag cttattctta atactgtcat agaaattttt
      181 atatgctata gttccttctt tatttgtttt ccaaactcca gtacaccact tttcaatccc
      241 ttcatagtca aagtataaaa tcaagttttg aacattattg tcaatacagt actgcattgc
      301 aattttagaa gcctctatct ctccagctac atttctcata cttactaaag ttttctcttt
      361 tcctttttca ctataagttt ttataacttc attatttttt aaaatcacaa cacccgaacc
      421 atacattttt actgaatgtt cataacttcc atctacatat gcttctataa actccttatc
      481 ttcttgaaaa ctcaattttt ctgacccatc aatatattct ttagcttctt gaaaagtaga
      541 aaaactctta tattcagctc ctgagaatcc atttacttgt tttttgcact catcccatgt
      601 attatataca cctatatttt tacctttttt cacagcataa aacttttgtt ttgccaaata
      661 tatcactccc actcttaatt tttatcttct tctctcataa atatatctaa atttataaga
      721 tagtttctat caaaatattt tactttcata tcttttttta taatatcaat atggtacact
      781 tctcctttta cagctctttg tcttatattt tcatttatat ctttttttat aatatcctta
      841 tcaacatcat tgttatccat tttagcatca acattcaacg cttttatagt atcaaatata
      901 acagatttta gtattaaact tctatattta tttaaatgtc gccttatata agtataaaac
      961 tcattgctat taacctttga aattttacaa tcgtgtaaat ctttctctaa tctctttaac
     1021 acacttatat tagttcctac atcaaaaatc acaaaacatc catagtcata tttgcttctt
     1081 aaaattctcc ccatagcttg ctttaccttt atggtcattt gaggataatt tatagtatag
     1141 tagtctatac catacttctt cattatagta aaatacaaag ggtcttttgg atttagattc
     1201 ggaagcttgt ctaaagttac acaaaccaaa ccatcccctg gaatgtctac gccttcaaaa
     1261 catccttttg agccaagtac tacacatttt ctatttaaat cgttaaggtg tctaatcccc
     1321 tttttatcag cataaatttc taagttaaaa ctatgtaagt attttttcaa tatttcataa
     1381 gttttttctt gtctatcttt actattaaaa agagctaatg tatgaccttc tgtaatcttt
     1441 gatatattag atataatttt actcatctca tttggaaatt cagaatttct gtactcacaa
     1501 atatcagaaa atccaactac agagactcta tttttataat caaaaattgg ttctataatc
     1561 ttttccacat tcttaactct gtctattcct aatgtatttt taaaataaga catactttca
     1621 gataaactca aagttgctga taaaaatatt cctttttcta attgagataa aatattttct
     1681 tcaaataaat ctgctatttt taatggcact actcgaaatt caaagtcatt ataattctta
     1741 tcaatttcaa ctatccttgc ataatcatcc ttttcatcat actctaaaaa gatttcaaat
     1801 ataatcttta tatcctctaa atctcttgtc tttgcctttc caaatttata tatatctgct
     1861 tctttatcta tactatcatc atcaatattc ctataaatta ttattattat tgaaactaaa
     1921 ttttttatta ttttctcaca actcaacttt atcttttcac tataggctct ataacttatt
     1981 tcagtatcta taccatcctt ttttagtttt cccacaattt cgtctatttg caaattcagt
     2041 tcccatctta aattataatt acttacatta ttatattcat tacaattacc aaactttaat
     2101 attgaatcta tctcttcaat aatgagattt atacttctag caattttttg tttatcttct
     2161 cttccaattt ttaatacatt ataaaattta tcaaaagcct ttatttttct catatttcta
     2221 ctatattttt tatatataaa gctactattt tgaataaact cgtaaggata aatttcttgt
     2281 aataaatacc ttaaagactt tgaatttata atgcttgaaa agaaatcata tcctttttca
     2341 gtcaaattat gtgcttcatc tactattata ttttctaaag gtttttcatc cttatatggc
     2401 catttagcta atagtgaatg atttactact gttatatgtt cttcttttaa ttcttctatt
     2461 ctgtttttat ataaacaatc ttttttgcat tttttaggtc tgcacatatt tgggtcacaa
     2521 cttacatttc ttaagtgagt acttatttcc ttgaagttgt caaacactaa ataatttatt
     2581 tcttctatat ctccatactt gccaccttca actaatctct tcaaaaatat taaagataaa
     2641 atttctcctt tagtagggtt ttgagactca taatcatcta tatatgcttc cagtctatct
     2701 atacaaatat agttattttt tccctttata tatccgtaac ttacttttcc atttagaccc
     2761 aaagaatcta acacatttgg aatatcttta tttattaatt gtatctgcaa ttcctttgtg
     2821 tcagtagaaa ttaataatct ttttttatta attctagcct ctaatattgc aggcaataaa
     2881 tatcctacac ttttacctat accagttggt gcctctatac atgcaatttt ttcttcatct
     2941 tcgctatttc taaacaactc tcttattgtt ttagtaagct catattgacc aggtctatat
     3001 tcatatgtaa atccttcttt attctcccaa atagttttgt attttaataa ttcttcataa
     3061 tttttttctt cttcatttaa aatttttaag gtttctcttt ccttcttctt ttcttctttt
     3121 gagtcaaata tattatattc tttttttata tttatattat ttgataaatc ataatttgcc
     3181 tcttctaaaa atttgctcca ttcccatgtt ggaagattaa attttttaag atatgaattt
     3241 attttaaaac tcaatggctc caaagtagtt ttttcatagt tattaaattt tactaaaagg
     3301 caatttacaa tatttattat atcaatagca tctgataaag ctctattttc cactttagaa
     3361 ttacaatttg ttaaagtatt ttttaagtat tctaagctat aatctttatg atatggttct
     3421 aaaataactg ctaattcaat taaatcaata aatttgtttt ttaactttgg catatgatac
     3481 tcaagaaact tttttttcag acttaaatca tgacatataa tgaaactatc ttctacaaaa
     3541 tttacaagtc tatcttccac cagttctatt tcattagcta tttctaaatc atttttatct
     3601 aaatttttac atactgagaa tacttctaca ggtacctcaa atttgttttt tattaatgat
     3661 tcataggtat atattttcca gtctttaacc ttgacagctc caacttctaa aatttctgaa
     3721 tttaaacaat ctaatccact aacttctata tctaaaaaaa caacatcatt caatacactt
     3781 attatattat ccatttttat atcccttcaa gttaaaccag cctaacttat ttacaatcat
     3841 ttctctttat attttatcat ttatatacac tttatttaag ataattaata tcaactttat
     3901 atcttacagt aaacttcttt catttattat ttgtatatgt gaatattttt aactataaat
     3961 attcaaacta tattataaga aatatacttt aaattttaaa taagaggtga aattgtgaaa
     4021 aaagatagcc taaaaaaata cattatgata ggagcatttg ctctaatact ttttggaatt
     4081 gctagtataa attttaaatc attagataaa acaaaaactc aaatttctag tccaacactc
     4141 gacacacatg aatatgattg gtattttaac cctagggagg atggtaaaca gccatcacct
     4201 ataaaagaag ctgatttttt taaaaaatac ggagcttatt atgtaggaaa tcccaatgaa
     4261 aaagttatat atctatcatt tgatgctgga tatgaaagtg gaaacactcc aaaattatta
     4321 gatacgctta aaaaacacaa tgctaaagct caatttttcg tagttgaaag ttatataaaa
     4381 agcaatccag agttaataaa gcgtatggaa aaagaaggac atttagtttg taatcattca
     4441 aaaagccatc catcaatggc aggtattaca gattttgaaa aattcaaaga agaaattaca
     4501 agtgtagaaa aagcatacaa agatgttact ggaaaagaaa tgcctaagta ttttagacct
     4561 ccaatgggta aatttagcga acaatcttta aaatatacac aagatttagg atataaatca
     4621 atattttgga gttttgctta tgtagattgg tatgaaaaga aacaacctac tcatgaattt
     4681 gccaaaaaca aaatttattc aagaactcat cctggtgcaa tagttttact gcatccaaat
     4741 tcatcaacaa atactgagat attagatgag gttcttacac attgggaaaa agaaggttat
     4801 aaacttaaaa cacttgatta tttaaataat aaaaaatagt atcataaatt taaaaaaact
     4861 ctatcatgaa ttatagtaaa aatatagtta tttataaata ctttaattct cgatagagtt
     4921 ttttattttt tatagctatt taaaaagaat tgtccattca tttatctctt tgtatcctat
     4981 attatgatat attttacctg ctacatcatt attataaaaa agacatggaa cttttccttc
     5041 taacaacaat tctttactca aattgtaaac catataactt gcaagtcctt ttcccctata
     5101 tcttttatct gtacttacag agaccaccat tgccaaaaaa ctagtttcca taccagttga
     5161 cacagtgcta atcatagtat catcatttct aataaaatat gctcttacat ttccctcttt
     5221 taaatgaact tttctttctt ctatgtaatt tttacttact ttatagtctg acctatttaa
     5281 aagttttcct atttcatcaa tatctttttc ctgagcattt tctattttat actccttcat
     5341 gttggaaaaa tctatttctt ttatttcttt taatacacaa aatttattat ctagtttttc
     5401 ataaaaatct ttgtatttac ttattagtct atctattaca catttttttc cacttatgct
     5461 ttctacatct aaatctttta tatgattctt catttctcct atattaaaat tatttttaaa
     5521 actataaatt ataagtgttg acttattttt taatattata gaggttatat ttccatcttc
     5581 tacctgagac catatatctt gatattgaga gctaaatcca taattctcaa cgtatccaat
     5641 tatgaataaa tttaaacttt cttcatgtga tacatattgc caaaattctt tttctaaagt
     5701 attatctatt tttacaatca agcagatatc ctccttctat gttaataatg atatacctta
     5761 taatttatag tatcatttaa aatcattata ttctaccaaa tattctaaat ttttaaatat
     5821 ctatgcaata tgttcgtatt tatttttata ttctgttact gcttcactcc aagcttcttc
     5881 tactgaaata cctctcttat ttgcaatttt ttgtgctaat tctattattt tcatagcttt
     5941 tcttgatata ttcatatata ttcctcctct aaattatcat aagttatttt aaagttaaat
     6001 ttctatagtc atgttatata tttatttact tatatcttac taattattta tgtactaaat
     6061 atgaactaca atagaagaaa tttagaactt aataaaacat aaaatttata tacagttcat
     6121 atatatttgt catatcacaa gacaagtgat ataatttatt atcattttac tgggggtgat
     6181 atgctatgaa aataagagct gaatatacat gtcttcttga aattgtacat gacataatca
     6241 aaggaaaatg gaaaactatc cttatatttg aattaagaca atctaataaa acattttctg
     6301 aattgcaaca taatatttat ggaataaatc aaaaaatgtt gattcaacaa ttaaaagagt
     6361 tacgtgattt tgggattatt gataaaataa gttctgctgg atatccatta catgttgaat
     6421 attttcttac taatcgtggt aaaaaaatgt taaaagcagt ggaaataatg caagaaatag
     6481 gaatcgaata tatgcttgaa catggtcaac aagaaattct agataataaa ggaatttgct
     6541 acaataagta atcctataca taccaaaaag taagtaattt acatttttta caatagtatt
     6601 ataataaaaa taaataggag gtataatcat gtctatagct ttaatattga ttgatattca
     6661 aaatgattat tttaaaaatg gaaaatgtga attatttcaa tcagaagaaa ctgctgaaaa
     6721 tgctaaaaaa atactactgt tttttagaaa aaacaattta ccagttattc atatacagca
     6781 tatatcaaca gataaaacag cttctttttt tcttcctaat actcatggtt cagaaataaa
     6841 taaaattgtt tttccactag ataatgaaga aattataact aaacacacac cagatagttt
     6901 ctttgaaaca gatttgcaaa gtatccttga gaaaaaaaat attactaatc tagtaatctg
     6961 tggaatgatg agccatatgt gtattgatac aagtgtaaga acagcaaaga aacttcgata
     7021 cgacataact ctaataagcg atgcatgtac aacgaagaat ttatcttgga ataatataga
     7081 aattaatgca aatactgtac atcaagtatt catggcatca ctacagaaca gttttgcaga
     7141 tgtaaaaaac acagataaat ttttatctaa ctatcacaaa taaaaacata cactatgtta
     7201 aaagagcaag tttgatttat ttaagcttgc tctttaatca tttaaaatta gatgctttct
     7261 atcattttta tatacataat atacgtttat acattttata ttcatatata actattatat
     7321 aaattttaaa ctttactata ttcacataaa aaatgtagta ttatagtata tataatatta
     7381 tatagaaggg ggtgctagat aaatgaacca tcatatattg attcaatttg aatatatagc
     7441 aagaatcatt actgctggtt tctgtggggc tttaattgga tatgagagaa agaatagact
     7501 aaaagaagct ggaatacgca ctcattttat agtggcatta gcttcagcac ttataatcat
     7561 tgtttccaag tatggattta atgatatagt gggtactcca ggtattggtc tagacccagc
     7621 ccgcgttgca gcacaagttg tcagtggtat aggtttttta ggtgctggat taatatttat
     7681 tagaaatcaa tcagtaagtg gtcttactac agctgctggt atatgggcaa cagctggtac
     7741 aggtcttgcc attggctctg gattatactt agttggtatt gtttcaagta tcattattgt
     7801 tattgtacag ataacacttc atagagatag gaaatggatg aaactcccta cagcagagca
     7861 agtttctctt aaaatttcta acagcactga ttccatagaa tatattgaag gtaaatttaa
     7921 aaaatctaat attgaaatta taaacatgaa tattaaacgc cttgatgggg attggttaga
     7981 agtagttata tatactaagt tacctaaagg atataaaaaa acaaatttat taaaattatt
     8041 tagcgataat cctcttatag aaactttaga aatataatat aaaaattgaa gcttaaaaat
     8101 aataatatat ttaatcaagg tcaatatata aataattacg ggatgagtct atataaaatt
     8161 catcccttgt tttttgccat tcatatatat tttttcaaga tatttttgtt catctattat
     8221 ctagccaagt aactcttgtg aactaaactt tttaatacaa aaacttcaat acaaaactaa
     8281 atataatttt tattaaatat tctacatgta tagtttattt cttgagtgaa aaatagattt
     8341 catattgtta agatactgtt aaatatttca attttctgac aagtatgttg aacaataact
     8401 tatcctagtg tattatttta gttgtagaat aaaactaaat cggcaaaact agagaaattt
     8461 agtgacgcaa agctataggg actaacgctt ataaaataaa ctatgagtta tgtcagccag
     8521 ttgccaaaaa gatattgttc tttttgtttt atgaaagttt tttaggggga tagtaatata
     8581 tatgtttaga gatgaaatgg ataaatgtac acacatgtta actgcttata tcagtagttt
     8641 atatgattat tgtgatttta tagatacaca gctagatgat tttatactag agtacggaga
     8701 aaatgtagta gaatcttgtt tacatcaagt gatggtattg gtaagtaagt ataattaaat
     8761 agaaattgtt ttctagagaa ccctttttag ttttatctac atttaaaaat gttattatat
     8821 taaaaaacta atctccccta tatgcaaagt tttcttccat ataacaattt cttcccattt
     8881 atactttttc acttgtatct ttaataaaat tcaacttcaa atattaatat acttcaaaaa
     8941 ttattctatt tcttatcttt acaccaattc ctatcagtat tatcctttat ataggttcta
     9001 actatttctc cacaatctaa acataaatct gaatatatag tagattgtac tggaaaagtt
     9061 ttatttataa ctttatctaa ttttccatct cctctatatt ttaatccaat agtttcagca
     9121 tatatgccac cagttgttaa aattcccttt tcaatattta cacttccaca attagaacat
     9181 ttcacattta taacccccta aacaattatt ttttcatgct atcttataat ttaaaatata
     9241 atttgctacc gattttataa cacagtggca aatcaacatt ttttcattct atttaccatc
     9301 taatccattg aaatatagct atttagttat aggaacttga atttccgtta caaactcctc
     9361 tacaattcca gtctcatgta tatctatctt atagatttct atagggtcag caattatctt
     9421 ataattattt tcttctataa atttaaacac cttctccata tatatcttat tattagaatt
     9481 actcccagta taatttaaag ttacataata tccttcatca aaaactatat tatatacttt
     9541 ctcattactt tccagaaaac aaaatacaga tttaaactca ttaaatatac ctttattaat
     9601 tgcttcagta gaaaaggcag aaccaatatt gttgtttcca agtatattaa atctatcttc
     9661 atattctttc tgaagttttt gaattaaaaa atcgaagtct tcatccctct ttatatcagc
     9721 atttaattct aaagcttttc ttttttctat atatacaacc tctatctcat ctatttttgt
     9781 attttgaatt acactttcaa ttgaactcaa tctttttata atattttcct tatggcttat
     9841 aaattcagct atttttttat caattaaatc gagttcttca tttaaaatat ttttcgtaga
     9901 ctctatactt ctgtcatcta ggtattcttt tattttcttc attggaaagt tcaagcttct
     9961 aaattctttt attaaattta atttccatat atctgatata ttatacattc tatatccatt
    10021 tatatctcta actggtctta gaatgcctat ttcttcataa tacattaatg aatctctacc
    10081 tattccatat attttagata tctctcctat tttatagtaa tctttcattt atataataaa
    10141 ctcctccgaa atcttatttt tattttaaat caacat
//
LOCUS       DAMKMI010000139        10103 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMKMI010000139 DAMKMI010000000
VERSION     DAMKMI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10103
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.145"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(98..1978)
                     /locus_tag="PC429_003114"
     CDS             complement(98..1978)
                     /locus_tag="PC429_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF3848597.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
     gene            complement(2182..8145)
                     /locus_tag="PC429_003115"
     CDS             complement(2182..8145)
                     /locus_tag="PC429_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF3848598.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     regulatory      complement(8269..8354)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8526..9929)
                     /locus_tag="PC429_003116"
     CDS             complement(8526..9929)
                     /locus_tag="PC429_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF3848599.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
BASE COUNT         2768 a         1877 c         1152 g         4306 t
ORIGIN      
        1 attattagat tgttaagata atgaaaataa aataagaatg catttaaata tataacttta
       61 tatctaaata cattcttatt ttctatttac ttaatattta tttttcaatt aaactcttaa
      121 tcttgtctat tacagatgag gctattcctc cacctacttc aaacaacttt gatgaatttt
      181 taccttttaa tgacgattct tgtgatttat ctaacgtatt tgtagcaagt ataactggtg
      241 aattgttttt agctgctaat ggtccaactg ctaaagcatc tataagttta tcttgatttg
      301 caactacaac tccatctagc ttagattgag agtagaattt atctattact ttggcattcg
      361 tctcttgtct agtttgacct gccaccctat tgtttgatat atctttgttt gctatttttc
      421 caacttgttc aatagccttg ttagatattt tttctactcc accaataaaa tatatatttg
      481 acaccttatt tgatttaata aattgttcag cttcattatc tacaccatct ttttgtgtaa
      541 gtactattgg agttttttct ttaccagcca gtgatgctat tgataatgaa tcagcttctc
      601 catttcctgc tcctatatac aacttgctta cattattcgt cttatctatt tcttttgcta
      661 tattaataga agtttgatat cttgtatctc cacctattct ttgtactgat atttttgaat
      721 ttacagcttt gatagcttca acaactgaat ttggcattga gttgtttcca cctatcacaa
      781 ttacttttga tggatttagc cttttcaatt cttctacagt tttttgagga agcttttcat
      841 ttgatgataa aagaattggt gagtttttta cactagcaag tggtgtagct gtaagcccat
      901 ccaccatact tttatcttct ccatttacta ttacaactgt atctgatttt gtccaaccac
      961 tttgagatat tttgatagct gtctcaaatc tatcagcacc ttttaatttt ttgactgtga
     1021 cattagatgt agaatcactt gaacttcctc ctccagagct tccaccacta gatgagccgc
     1081 ctgaattact tcctccacca ttatttccac cattgccatt atctggtggt aattcatccg
     1141 gttttgataa ttcattttca actatgtatt ctaaactttt tggaagtgta acttcaacat
     1201 atttgtttat tgtagctttt cttcccttag ttgttttttg tgctaatcca agttttaata
     1261 ttacatttga accactttct tgtgtttctt tactaacaat agaatagttt atcctaatat
     1321 tgtttaataa atattcaagt gcttcacttt tctctacctt atacttttca gggtcatttt
     1381 tactagaacc agttccacca attggtttta cttcactctc attaataagg gtaaatatta
     1441 atccttcatc agagcttata ttttcataat ccactgtttt gttgttatat gttacaattt
     1501 ttaaagctgg tttatcattt gttccttttc ctaaaacttt atagtcagca tttgagttac
     1561 tgccagattt ttttacagat attcttccta tattcaatct atttccgtct agtaaatctt
     1621 catttgatgt aacatatata tctaatgtag ttgtattact tgatttgttg tattctactc
     1681 tacaagtagc atttgcattt ccacttttta tctgagattt tatattatca gaccaagtaa
     1741 tcttttcttt ctgctcatta tctactactt caacctcacc ttgtactttt atactggctt
     1801 gaaatgaata tactatgttt tctactgagt ccacagatat atctatagta tttactttct
     1861 catacgtttt tgttccttgt aaaactggag ctgcatatgc acatgtagca ctaccagtta
     1921 cagctactgc catagttgat actgctatag ttcttataaa agattttctt ttactcatta
     1981 acacccgtcc ttatctatca ttaactaagt ggataaattt acttatccac ctagctttta
     2041 tatttaattt ataaattacc ctaaatttat ggttacattt atatacttta cttttaatat
     2101 ctactatttt aaaactattc tagtacctaa tcttattaga tatacaatgt tagtattttt
     2161 acgtttttag agttccacct actatttatc tctaacaata ttacccttta ctttattgct
     2221 tacagttata accttcttat tactgatatc aatagaattt cttgtagctt ttctagcttt
     2281 actattacta tcagtaagac ttattgtctt taactcatca taattcttag ctcctacagg
     2341 tacatacatt gtatcactta caaatctttg acctactgga gtatctcctt gaaggtcatt
     2401 gtatctatat agttcagcct taaccttatc acttaaagaa tcaaagtttt cttttggcat
     2461 atagtaaacc catgtacctt tactgatttc acctagttca cctttttctg gaacttctgc
     2521 aaataaaagt gcttctccag atatagtctt attgttttta tctataagta gtggtaaatt
     2581 aaatgctgga tttccactgt attcacctgt aataactatt gaacccgctg gaatcttacc
     2641 tgtttcatct tcacttgtgc tagagtcatc actttgatat acataatctt ctgctaattt
     2701 tccaattcca ttagtacgag tttcgtctgc attatttatt atgccaattt ctatattatc
     2761 acctggaggt cctattatat ctaattctgc aacttctata ttagacttat tatttgagat
     2821 tagttttaca tatctagctt ggtatgtcca aagtttacca tctttattaa atgtaactct
     2881 agcttcatta tcttggtctt ttcctccacc aactaaatct tgtccatatt caaaagttcc
     2941 ttgacttaca tcttcccagt tctttttatc attacttact tgtatcttgt aattagttaa
     3001 aggattgtat gttgtgctag tattctttct aaagttaaat aatcctttta atgagaactt
     3061 tctagcttct gtctctggtt ttgtatattt tagtcctact ataggtaatg tttctttcat
     3121 gtctactatt atatatggat tttcttctac atttatatca gggtcttttt gagggtcttt
     3181 gttccactct gctttactta ttatctttcc tttatatgta gtacttgcat cattatcttt
     3241 tatattatca atttcaggat tttgtaatgg tccatgtgag tcattttcag ttctaacatc
     3301 accgtcagaa acagtatttg ttatgaatga ccatgctgat ttatttattt tcccttcatc
     3361 tgataatttt acagtaccaa cttcaaattc ttctgttgga tttaggtgat aatcatatgc
     3421 aactacctta tatgtaaatg ctctgttatt aagaggagat atattatcag taaatttagt
     3481 cttgctattt ccttttgcat ccacaaatcc aactgttctg tattttacct gtgttccatt
     3541 ttcaccatct aaataatttc catcacttct tattatttcg tatcctagta tcttatcttt
     3601 atccttatct acatctaggt ctatagatat tttattagat ttaactagag aaccactttg
     3661 tattccatca gaaaaactag cacttacttt agtatctttt gccatactag ctatattttc
     3721 atttaaaatc tttcttctag cttcatcatt tacatattgt atctttcttg attctttttc
     3781 gtagttcttg ctatccatta tactgtttaa ttctggtgta atttctactc cccatgcctt
     3841 aaagtaatct cttaaatctt tttcaacaac acttgatgcc attgctacta gcatttggtc
     3901 ttttggtaaa ctattaattg ggtcactact tgaaagtgct ctgtattttc ttgaaatctt
     3961 tgcatagaat gtatcattac ttaaatcagc atctgagtta ttctttaaca ttgaacccgt
     4021 aaaatcatca tcataagcta gatgtaattg ccataacatt ccaagccttt caaatacatt
     4081 gcttggtata cctaagctat ttgaagttac atgctcatat atcttatcca ttgttgaacc
     4141 ttcaagtctt gaatgtgtct tatcatcaaa ggtctgagca agtaaagcaa gtacattgtt
     4201 actagtttcg ctatatgtca tctttccaat atctgtaaca tgtcctatct catgacctat
     4261 tccccatcca aataagtttc ctcctgtagc ttttccacta tcatctattt ggaaaggtac
     4321 tccattcata agccctggaa ctgaaccata ttctactcct acatgtaatc cagaagcata
     4381 cataaatgct ccaataaaca ttctttggta ttttatgttc atacgagatt ttggagccat
     4441 atcattttta gttaagttaa attcattatc atctacagtt ccattaccat ctaaatcttg
     4501 tgttttaaat actcctcttt ctgagtaaac taaatccatt atttgttccc atgcaagcat
     4561 actattatat aatctatcta cttgtgtatt taaatcagaa tttccttttg atatctcttg
     4621 atatactgct gttgcaggaa catttaatgt tactttatca ctttcaatat ctgtagtgtt
     4681 taacacacca gttttttctg aatatccata tgaattacca acatttgttc ctggataata
     4741 tggttctcct tttacagcat ctttatattt agctaattct tctatatatg cttttatctt
     4801 tttcttagat tcagaaactc ccgcaggact cttatcattt attgttccat ttagattaag
     4861 atgtggtatc ttagtagctc ctgctacacg tacttttata tctcctccac ttggagtatc
     4921 ttcagcgtat cttatgtata aacttcctcc acattctata tctaaactat gaagtttgct
     4981 taattgaatt acattttttc cagtgttaag atttatttct ccactcttat atgcaccaga
     5041 ttctccatag tactgtgtat atatcaactt aggagttttt ccacttttac ttccaacata
     5101 tactgtgatt tcatctcctg ctctagcagc tactcctaat gcctgccagt cattttgcat
     5161 acctaaatta ttggaattta cagtatactg tccgttttta tctttatatt ttcctaattt
     5221 atttatttct tgatttacag tagttacatc agtaagtgtg gctctatcat tatataagtc
     5281 ttgagctaat tttaattctc tctcaataac tgtcttatat ggatgatatt ctccactagc
     5341 tttatctgga gtatttaatc tatcttttaa ttcagttatt ttttcttgag ttacatcttc
     5401 ttttaattca acttgtaggc catccataaa tagatttctt acatcatctt ctatactgtc
     5461 atactcataa aacttaagtt ctgatatatt agacttatat gaataggatg aacaaacact
     5521 tacttgtatc ttggtagcag ttattggttc ttttaatcta accattacat acttattatt
     5581 atttacaggg tctgtctttg tttcaaaagg aacatccccc agattgtgta attgaccatt
     5641 ttcatcccaa tatcgtacct caaagtcatt tattgtctta tcagaaacat caaaacgagg
     5701 tgtagccata aaagtatcca tagtataaga tttatcaaaa gttattattg gtcctctatt
     5761 cgttccctca gctgcattat atgttgggaa tgtccaatgt gttgtaaagt ccccatctac
     5821 tacattgaat ttatcaaatg gatttttatc actatattca cttttgtcat atccatctgg
     5881 ataaacaaca tctacaatgt gatttgttgg tattccaaat ccaccatttt ctttaggagt
     5941 attaattaat ctatagttag gtgttgttgg tgcatccatg ctttcagttg tacctatata
     6001 tgttttagac attccactag ttcccaagtg gttagtagct gtcattctga cttcatatgt
     6061 tgtattatcc ttaagacctt ctatctttga acttgtccca cgaataagct tatcattgta
     6121 ttttatccaa gacccattag agcctttttc tttatagtac aagtcaaaat ctttagcttg
     6181 tttatgattt ttccagctta catcaagaga tttaaattta cctttaacat ttataccttc
     6241 tggtggttct ggttttgtgt ctggaacaac ttgaaattct aatatactat ctgggctaaa
     6301 ttgtataggt ttatagtttg aatctacatt ttctggtact ccgtctaagt tttctggtgt
     6361 tgcatcttta taaccactag accaatctcc atttaaagat tgtatgctaa ctctatatag
     6421 tccatatgct tctatatctc taacttctat tgtattttta ctagtttgta attttttagt
     6481 agataatact tttcccttac tatcaatttt gtctagtata agttcgtatg ctgatacatt
     6541 tacttcatta ttccaataaa aagatacttt ttcatttcca acagcagtgc tagtcgatat
     6601 tttatttatt ttaggtatgt tcatctctgg ctttggtatt tctttatata cattgtttaa
     6661 aaattcaact tttgatattt ctgcaagatt tctatcgttt gctgttgtag ctgttatttt
     6721 tatagttatc ttttttactg ctacttgttt accaagattt attactatat cactattttg
     6781 aacatctgca tatgtttcta atgctgacct actatatgta gatgttgtag agttatcatc
     6841 tttactgata ttaccctctt cttttggagt atcttcatct gaattaggtt tatccattga
     6901 tgaattttct ttatcattat ttttatcttg attggtatta gagtttgctt cttgtttatc
     6961 gctattttcc tgttgtttat cattattttc atctttatta tttgagttat tatcagtctg
     7021 gtttatattt tcatctttat tatttacttc ttcttgattt acttcttctt gttcgttatt
     7081 tacttcctct tgttgctcct catcttttaa gtttttcata tattctttgt tttcataagg
     7141 aatctgttga acattaccat ctttgtcagt aacctcaact attccttttg ctggagcagt
     7201 accttctgga gcttttatat tgatgacttc catattaagt gcttctgata aagctatatc
     7261 cattactgct ggtttttcct cagatatttc atcatttttt gaagatattg taactccatt
     7321 attttctcca ctaaataaat tttctacatt accattaata tctacatcac tatttccttt
     7381 taattcaacc ttacttgtat ctactatagg attggtagat actatacttt ctaggctttt
     7441 tgcatcttca ctttgtccta aatttttctg tacataatct aagtcaacaa tatctacctt
     7501 tccatctcta tttaaatcaa attctctttt atttgaatct atattctcta aaaccttatt
     7561 ataatcactc ttatctactt taccgtctcc atttacatca cccattaaga acaattcata
     7621 atttaaaggt ttatttttat tatctacact tgctctattg cttaataaga ctctttgtga
     7681 ataacttgat atatcaatgt ctttaatttc tttgttctta aatccatctc cagagacttc
     7741 tacattgtat tttccaacag gtaaattatt aaatacaata ttataatatg atatattctc
     7801 atctttattt tttatttcac ttctacttgc agataatttt tttatagtat attctatatt
     7861 atctgtccca acatttactg ttcctgaatc tttttctcca agctttatta ttccactatt
     7921 gttactatca tcttttaatc gtaactgcat attagtcata ctttctgact cgctattttt
     7981 tattggcagt gaaaaattta aatctacttc aagactcccc cttaactttt ctgtaactga
     8041 tttttcaact gcccttagct tcccctctaa tataggttct gcaaaaacgt ttagtggtgc
     8101 actacacata gatactgcta gtacacaaga tattgccttt ttcatgaaat aacaatcctc
     8161 ccttaataaa tatctaattt ttcatgaatt taaatactat aagtttcaga caataaaaaa
     8221 agacactcaa agagaatctt aattataaaa taaaaagact ttactctttt tagcaactgg
     8281 ctgatatggc tttaaaagct ttaatcccta tagttttgcg tcattagatt tccctaattt
     8341 tgccatttaa ttttcactac tttcttaccc attttatact atatttaaac tttttaatat
     8401 aattttttta gattaaatta taaaaaaata acatttatat taatttatta atataaatgc
     8461 tattttccat tataatttaa ttatttatat agcttcctta caagcataat tttgtgtata
     8521 ttttcttatt ctttatatct tccataaaat tcatgtgtag catatagatt cttatctcct
     8581 gttatagcta taccaaatcc cattatttca taccctttat gcaacatatt agttccttct
     8641 tctggatttg tcttccactc attaaaaata aagtttccta tatcatctgc atctctattt
     8701 gtatatacag gtttatctgg aattttatat ttaattatat tttcaccttg aactgctata
     8761 tatccaggtt taatctcact ccaatctaag tatttcaaaa aagtagaata tgaatttttt
     8821 ccatttatcg tatgagataa agtcttctta tttgccatta aattagacca atcatttgct
     8881 agaccctcta atctgcttgc tacagataac gctcttactc cattctcttt tctatactgc
     8941 tttgtaagag catatacttt accctttatt ctctctttaa atgctctatc atttggattg
     9001 taatataaac cttgttgatt tggtgaagct tgatttatac attgcttgat aacattctga
     9061 tttatgcctc ctagtacagt caatttgtct gcaccactta aaatactctt gttactgcca
     9121 ttttcaacta aaactacagg agcatttttt gctattgtag aggcagccaa agcgtctgtt
     9181 aattgtaagc ctttacttag ataaaattct ttactatttt tataaaactc ttgtataact
     9241 atcttattag tttcaaatct atctgtgcca cccaatctaa tagaatttgt gctcttaact
     9301 aattcgtcgc ttattatttc actagaacca agcgcataac tctttaagcc tgtcgtgtca
     9361 aatcccacac tatttccatc tgtaagtatt ataggtactc catctctagc agctactggt
     9421 gatgcactta tcgcatctgc ttctcctgta aatccattgg ctataattac tttatctatt
     9481 ggcttaattt ctttgatttt ttcagctatt tttatacttg tatcaaatct attttcacca
     9541 aatattcttt gtacaacctt ctttttggat agtaactgat tctcaacatt tttacttatt
     9601 gttgattctg aacctattat atacacagtc tttgctttgt ttaatctttc catagttgtg
     9661 tttggaatct ggttttgttt tgtcatcaaa ataggcgcat ttaaggctcc agctaaacca
     9721 cttgcactta gaccatctgc taagctatta tctgagttga ttagtattgc tgtattgtag
     9781 ttttgcttat ctgctatttt tgctgctgtc tcatatctgt cattcccttt tatttgctca
     9841 actgatgtca acgcatttac cataggagta tttaccatta ctaaaaatag tgtaagtcct
     9901 atagctaata tctttttact attcttcata ttattttcta cccccaatgt acttttcata
     9961 ttttttatta gtactcatac ttatttttaa tcaaaagtta caattttgtt accatatttt
    10021 atatatgata ccatagttat ccatacaact caaacaatat acctaaagtt tttattctct
    10081 ataaattcaa aacattataa ttt
//
LOCUS       DAMKMI010000140        10039 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMKMI010000140 DAMKMI010000000
VERSION     DAMKMI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.146"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            17..1057
                     /locus_tag="PC429_003117"
     CDS             17..1057
                     /locus_tag="PC429_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF3848600.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTERLIIKVIRHSSNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIESVRQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYT
                     EDICIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKA
                     KEYVKIAIDDCI"
     gene            1196..1648
                     /gene="tsaE"
                     /locus_tag="PC429_003118"
     CDS             1196..1648
                     /gene="tsaE"
                     /locus_tag="PC429_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF3848601.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDDGREMILTPKGEFYKEIVEELIK"
     gene            1648..2364
                     /gene="tsaB"
                     /locus_tag="PC429_003119"
     CDS             1648..2364
                     /gene="tsaB"
                     /locus_tag="PC429_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF3848602.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2354..2830
                     /gene="rimI"
                     /locus_tag="PC429_003120"
     CDS             2354..2830
                     /gene="rimI"
                     /locus_tag="PC429_003120"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF3848603.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2836..3852
                     /gene="tsaD"
                     /locus_tag="PC429_003121"
     CDS             2836..3852
                     /gene="tsaD"
                     /locus_tag="PC429_003121"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF3848604.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4149..6857
                     /gene="hpdB"
                     /locus_tag="PC429_003122"
     CDS             4149..6857
                     /gene="hpdB"
                     /locus_tag="PC429_003122"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF3848605.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKSAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATADGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6861..7118
                     /gene="hpdC"
                     /locus_tag="PC429_003123"
     CDS             6861..7118
                     /gene="hpdC"
                     /locus_tag="PC429_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF3848606.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7111..8061
                     /gene="hpdA"
                     /locus_tag="PC429_003124"
     CDS             7111..8061
                     /gene="hpdA"
                     /locus_tag="PC429_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF3848607.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLGELQDIFLDNGIACYVGHETAF"
     gene            8376..9338
                     /locus_tag="PC429_003125"
                     /pseudo
     CDS             8376..9338
                     /locus_tag="PC429_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(9437..>10039)
                     /locus_tag="PC429_003126"
     CDS             complement(9437..>10039)
                     /locus_tag="PC429_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3848608.1"
                     /translation="SIIIYSSNKKNLYPIKNIKLGSLLIIYFMISVIVPTSFILILQG
                     SQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFNKFGKKLGVIILGICWS
                     LWHLPLIFTLYTPETPILGLILRSIHIVGISIFLGYLYIKTKNIWFCAIIHILNNSAF
                     LITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         3928 a         1038 c         1871 g         3202 t
ORIGIN      
        1 atgggagtgt atattcatga acttatctaa aaagataaaa aaattatttt tagatgataa
       61 agggtattat tttaatagta attggaaaga tattcatatt ggtatttata atacaaaatt
      121 aggagattct ttaaagtata tcactaatga atatattgaa aatctggaaa gggtaaaata
      181 tgactatcgt gaattaaaaa atatagaaaa tcaaactgat ggtatgtgta ttttagcaat
      241 aaggcagaat tacaaggcat tagagtatgt aaaaaatcaa accgaagaat tatgtttaga
      301 agcaataaag tataattata aagcacttga atatgtaaaa gagcaaacag aatatatatg
      361 tcttgaggct ataaaaaagg attgtaatgc acttaagtat gtacgaaata aaacagaaag
      421 gcttattata aaagtaatac gtcattcttc caatatagat gttgtaagta tactaaaggc
      481 tttagagact caaacaagaa gaatatgttt agaagcaatt aaaaaagatg gcaggtgtct
      541 tgcatacgtt agagaacaga gtgaagaatt atgtatagag gccattaaac aaaattataa
      601 agcattaaaa tatgtagaaa atcaaacgga aaaaatgtgt atagaatccg taagacaaaa
      661 tggtatggca ttacaatatg taaataaaca aactgataaa atatgtatag aagctgtcaa
      721 acaagatgga agaagtctac aatttgtcaa taataagaca gaagaaatat gtataaatgc
      781 tataagatat ttaaataaaa aatataatat taaagatgta ctaagttaca tagataagta
      841 tacagaagat atatgtatag agattgtaag acaaaatgga aaaatgctta tgtatataaa
      901 aaatcaaaca gaaaaaatgt gtattgaagc agtaaaagag aactataagt ctcttaagta
      961 tgtaaaagag caatctgaga gaatatgtaa agaagcattg aagcaaaacc ataaagcaaa
     1021 agagtatgta aaaattgcta ttgatgattg tatataaggt gtagatacag ccatcaatag
     1081 tgaaagataa aaaaagataa agacaagtac tttataataa tctaggctta tactataatt
     1141 aattaagcag tgatataaat tcacatgacg attatatcaa aatggagatg aattgatggc
     1201 aaaaatatat ttagaaaatg aaaataaaac tagagaaata ggatataaat taggtaaact
     1261 tttaaaagaa ggcagtgtta tctgtttagt aggagattta ggagctggta aaactacaat
     1321 gacacaaagc ttggcagatt cattagggat agaagattat ataacaagtc ctacttttac
     1381 tataataaat gaatatgaag gaaaaatacc tttgtatcat tttgatgttt atagaatagg
     1441 tagtagtgac gaaatgtatg atattggtta tgatgaatat gtaaattcaa atggaatctg
     1501 tataatagaa tgggctaatc taatagaaga tatacttcca aaagaatatt tgaatataga
     1561 attgagatat aaagatgacg gaagagaaat gatattaact ccaaaaggag agttttataa
     1621 agagatagtt gaggagttga ttaaataatg aaaatactag gtatggatac gtcttctatg
     1681 gcagcaagtg tagcagttgt tgaagatgat aacttaatat gtgagtttac agtaaacaac
     1741 aaaaaaactc attcacaaaa gcttatgcca atgatagaaa atatgttgag tatgagtgat
     1801 ttaagtataa aagatatgga tttattagct gtatgtatag gtccaggctc gtttactgga
     1861 cttagaatag gtatggccac agtaaaagct atggctcatg taaataatat accaataata
     1921 gcagtaaatt ctttggaatc tctagcaaat aacataaatt tctgtgatag aaagatatgt
     1981 tgtatattag atgcgcagaa aaatcaagtt tattcagcta aatacaagtt tgaaaatgga
     2041 gagatgatag aattagatgg tgtagatgtt gtagagtttg atgctttagt agatgaaatt
     2101 gtatcaacta atgaagaatt tataatagtt ggagaagctg tatacaaata taaagataag
     2161 ttagagaata taaaaaatat aaaaatacct tcaccagcta ataatgtatc taacgcagga
     2221 agcttatgct ctttagcatt aaataaatat aataaaaata tagatgtaca tacttgttat
     2281 acgataaatc ctatgtatat cagaaaatct caagctgaag tacaatatga tgaaaaaatg
     2341 aagaggctaa ataatggaag ataatacaaa actaatagta gatgatataa aaatagaaga
     2401 aatgactaca gaagatatag atgaagtttt tgaagtagaa aaaaattgtt ttgaagatta
     2461 ttggtcaaaa gagtctttta gaaaagagtt gtctaatgaa gtagcaaggt atatagtagc
     2521 taagttaaat ggaaaaattg tcggatatgt gggaatttgg cttatacttg atgaagggca
     2581 tataaataat gtggctgttc atagtgacta tagagggaaa aaaatagggg acaagttgat
     2641 aaaaggcata gttgatttat gtaaagataa taatattgca tctatgacat tagaagttag
     2701 agcatcaaat aaaatagcac aaaatttata tagaaaatat ggatttaaaa tgggtggaat
     2761 aagaaaagaa tactacagtg ataataaaga agatgcaatt ataatgtgga accaattaaa
     2821 agaggtgtag agtttatgag tgatataata acattagcta tagagagtag ttgtgacgaa
     2881 acagctgcat cagttttaaa aaatggtaga gaagtacttt caaacataat ctctactcaa
     2941 atagagacac ataaaaaatt tggtggtgta gttccagaag tggcttcaag aaaacatgtg
     3001 gaaaatattg atatagttgt tcaagaagct ttagacaaag caaatatagg ttttaatgat
     3061 atagaccata tcgcagttac atatggacct ggactagtag gtgctttatt ggttggtcta
     3121 tcttatgcta aagcacttgc atatactttg aatattccac ttgtaggagt aaatcatata
     3181 gaagggcatc taagtgcaaa ctatatagag cataaggatt tgaaacctcc ttttataaca
     3241 ttaatagtat ctggaggtca tactcacttg gttgaagtaa aagattatgg caagtatgaa
     3301 attttaggaa aaacaagaga tgatgcatct ggagaagcat tcgataaaat ttcaagagca
     3361 atgaatttag gttatccagg aggacctatt attgataatc tagccaagaa gggaaataaa
     3421 catgctatag agtttccaag agcctattta gaagaagata gttatgattt tagttttagt
     3481 ggattaaaat cttctgtatt gaattatcta aatgggaaaa gaatgaagaa tgaagaaata
     3541 gtagttgaag atgtagcagc atcttttcaa gaggctgtgg tagaggtttt atctactaag
     3601 gcactaaaag cagtaaaaga aaaaggatac aatatcataa ctctttctgg tggagttgct
     3661 tctaattcgg ggcttagagc taaaataaca gagcttgcta aagacagtgg tattactgtt
     3721 aagtatcctc ctctaatttt atgcacagat aatgcagcta tgatagggtg tgcaggatat
     3781 tataatttta taaacgggaa aacgcatgat atgagtttaa atgcagttcc aaatttaaag
     3841 ataaatcagt agagttagat aaatttgtaa atataaaagt attgtttcaa aaaagtaagc
     3901 agaagtgaaa ttaaatataa taaaaatcaa tatagtatat attaaaactt gaagtaaaac
     3961 tatttataat ctttatattc caatgaattg caacacattt taggatatac aggttgtctt
     4021 tggcttagat ttgcttcaag tttaattttt tctatagaaa gattatttta aaaagtgggt
     4081 aatataccct tttttttcat tcttaaaaag ttttatacta attatagaaa gattaaggag
     4141 gggaagaaat gagtcaaagt aaagaagaca aaataagaag tattttggaa gcaaaaaata
     4201 tcaaatcaaa ttttcaaaat aaagaaaatt taagtgaatt taatgaaaaa aaagcaagta
     4261 aaagagcaga agacttatta gatgtatatt ataatacatt atctactgca gatatggaat
     4321 ttccatattg gtataataga gaatatagaa aatcagatgg tgatatacca gtagttagaa
     4381 gagctaaagc tcttaaatct gctttttcac atatgacacc aaatataata cctggtgaga
     4441 aaatagtaat gcaaaagaca cgccattata gaggttcttt tccaatgcca tgggtaagtg
     4501 aaagtttctt tgttgctcaa ggagaacaaa tgagagaaga ggcaaagaaa ttagccagta
     4561 acacagctga tgaacttaca aaatttgggt ctggcggagg aaatgttact gaaagttttg
     4621 gaaatgtcgt ttcaatagct ggtaaattcg gaatgagaaa agaagaagta cctgtacttg
     4681 taaaaatggc aaaagaatgg gttggaaagt ctgttgaaga tttaggtttt cactatgaaa
     4741 aaatgatgcc agattatgat ttaaaagaaa acctaatgag tacacttata tgtatgtttg
     4801 attcaggata tacacttcct caaggtagag aagttataaa ttatttttat cctttaaatt
     4861 atggattaga tggaattata gaaatggcaa aagaatgtaa aaaggcagtt gcaggaaatg
     4921 catctggaga tgggcttatt ggtatggacc gtctatattt ttatgaggct gttattcaag
     4981 ttattgaagg attacaaact tggattttaa attatgcaaa acatgcaaaa tatttagaaa
     5041 gtatagagac agatttagaa gctaaaaaag aatactcaga tttagtggag atattagaac
     5101 atattgcaca taaacaacct cgtacattta gggaagctct tcagttgact tatacaattc
     5161 atatagcatc agtaaatgag gatgctatat ctggtatgtc tataggccgt tttggtcaaa
     5221 ttttatatcc ttggtatgaa caagatatag aaaaaggatt aattacaaaa gaagaggtta
     5281 tcgaattatt agagctttat agaataaaaa tcacttgtat tgactgtttt gcttcagcgg
     5341 gcgtaaatgg aggagtacta tcaggaaata catttaatac tttatctata ggtggattaa
     5401 aagaagatgg ttctacagga gctaatgaac tagaggagtt attattagaa gcaagtatga
     5461 gatgtagaac tcctcaacct agtttaacta tgttatatga tgaaaaactt ccagaagatt
     5521 tcttaatgaa agctgctgaa tgtacaaagc ttggttctgg ttatccagcc tgggtaaaca
     5581 actcaaatgg aacaacattt atgatgaaac aatttgctga tgaaggtatg actgttgaag
     5641 aagcgagagc atttgcatta ggaggatgtt tggaaacgtc tccagggtgc tggaaacagt
     5701 taactttaaa cgggaagaca tactctattg caggtggagc aggtcaatct gctggctctg
     5761 gagttcactt tatagctaat cccaaaattt tagaacttgt tcttatgaat ggtaaagacc
     5821 atcgtatgaa tatacaagta tttgagcctc ataataagcc attagatact tatgaagaag
     5881 tcatagaagt atttaaagat tattataaac aagcaataaa tgttttagaa agagcaaata
     5941 acattgagtt agatatatgg agaaaatttg atacttctat aataaattca ctattaaagc
     6001 cagactgttt agacaaaggt caacatatag gaaatatggg atatcgttat aatgcaacat
     6061 taaacgtaga aacttgtggt actgttacta tggtcaattc atttgcagca cttaagaaac
     6121 ttgtatatga tgacaaagca tttacaatag aggaaatgaa ggatgccata ttaaataatt
     6181 ttggatttaa agatgcatta gaagtaggaa attattctat ggcagaccaa gtaaaagtag
     6241 ataagactgg taaatatgat gctatttata aggcctgtct tgatgcacct aaatatggaa
     6301 ataatgattt gtatgcagat aatattctta aaaactatga ggtatggtta tcaaaagtat
     6361 gtgaagaagc acaatcttta tatgctaaga agatgtaccc ttgccaaata tctgtttcta
     6421 cacatggacc acagggagct gctaccttgg ctactgcaga tggtagacta agtggaacaa
     6481 catattcaga tggttcagtc tctgcatatg caggaacaga taaaaatggg gtatatgcat
     6541 tatttgaatc tgcaactatt tgggaccaag cagtagttca aaattctcag atgaatttaa
     6601 aacttcatcc aacaactata aaagggcaac aaggaactaa aaaattatta gatttaacaa
     6661 gaagttattt aagaaaaggt gggttccata ttcaatataa tgttgtagat tcagaaacat
     6721 taaaagatgc acaaaaaaat cctgacaatt atcgtcaatt aatggtacgt gttgctgggt
     6781 ttactcaata ttggtgtgaa ttaggtaaac caatacaaga tgaagtaatt gctagaacag
     6841 agtatgaagg ggtgtaaaat atgagaaagc atagtgattg tatgaatttt tgtgcagtag
     6901 atgcaaccaa aggaatttgt agattatcaa aacaaatgat taatttagat gatgcagcat
     6961 gtccagagat aaaagtaatg ccaaaatgta aaaattgtaa aaattttgtt gaagctaatg
     7021 atgaaggtat aggcaaatgt gttggcctag agaaagaaga ctgggtatat tcaacattga
     7081 atgcaattac ttgtgaagga catgtgttta atgagtagtc aaaagcaatt agaaggcatg
     7141 attttcgatg tacaaagctt ttcagttcat gatggtccag gttgtagaac aactgtattt
     7201 ttaaatggat gtccactgag ttgtaaatgg tgtgcaaatc cagaaagttg gactgtaaga
     7261 ccacatatga tgtttagtga attatcttgt caatatgaaa atggatgtac tgtatgtcat
     7321 ggtaagtgta aaaatggtgc attgagcttt aatcttgata ataagccagt tatagattgg
     7381 aacatctgta aggactgtga aagttttgaa tgcgtcaact catgttatta taatgcgttt
     7441 aaattatgtg caaaacctta cactgtagat gaacttgtgc aggtaattaa acgtgattct
     7501 aacaattgga gaagtaatgg aggagtaaca tttagtggag gtgagccact acttcagcat
     7561 gaatttttac atgaagtatt attaaagtgt catgaggtaa atatacacac tgccattgaa
     7621 acgagtgcat gtgtatcaaa tgaagttttt aataaaatat ttaacgatat tgattttgca
     7681 tttattgata taaagcatat ggatagagaa aaacacaaag aacaaacagg tgtgtataat
     7741 gatttaatat tagaaaatat atcaaacctt gcaaattctg attggaatgg aagattagta
     7801 cttagagtac cagttatctc tgggtttaat gatagtgatg aaaatataag tgatattata
     7861 tcttttatgc acaaaaataa tttggtagaa attaatctat taccatttca cagattagga
     7921 gaatcaaaat ggattcaatt aggcaaggaa tatgagtatt ctgataaggg tgatgttgat
     7981 gaaggtcatc ttggagaatt acaagatata tttttggata atggtatagc ttgttatgtt
     8041 ggtcatgaga cagctttcta aaattaaata caagttttaa ttaaaaaagg atgttacact
     8101 aaaagctaga gttacatcct tttttatgtg gtttttatgt agaaataatt aagtaaagtt
     8161 ttatatattt tatatattgt ttatattgct ttatgtccat aaatattgct ttaaatatac
     8221 tttatatatt ttttatagtt taaattacag ttagagaata tattacgttt actttattga
     8281 agcagaagtt ttgtggtaaa atactactat gaaaaaattt aatagaatat attttagaaa
     8341 caactaaaaa taatagttag gaggaagaca tctatatgaa caaatcatgg agcaacttga
     8401 ttattaagaa taaagacaaa aaagtacgtt cagggtggat tattatagct gttatggcta
     8461 ccttttacat atttcagtat tatttaagca tgatacttgt ggaaataata agaaaaatcc
     8521 tggttgctac aggggacatt aatttaaaaa caaactattt ttcagaatat gctaattggt
     8581 taaatgatgt tgggttacag attgtatttc aaattctaac agattcactt atgattatta
     8641 ttcctataat tgtatggaga tttattatga aaaatcctat tagtgaaatg ggattacatt
     8701 cttatagaat taagaaaaaa gaaggatatg taggaatgtt attaggaata tttaattgta
     8761 cagtaatctt tattataaca ataacaattg gtggtggtca tgttacttca tgggtaccaa
     8821 aaatttcacc tttaacagtt tcttgaatac ttgtctttat tttagttgct tttgcagaag
     8881 aaattttaaa tcgtggtttt tttatggcag ttttacgaag atgtaaaaat atttatttta
     8941 taatgattgt tccatctgtt atttttgggt tgattcatat atggaatcct gatgttactt
     9001 ttttgtctgt aattaatatt ataattattg ggattctttt ttcatatatg tttataaagt
     9061 cgtcgaatat atggatgtgt ataggttatc attttacatg gaatgtattt caggggatta
     9121 tatatggtat gcctgtaagt ggacttcagg ttccagggat tataactaca caaatcactc
     9181 atggtaatct attaaatggt ggtatgtttg gtattgaagg tggaattttg actacttttg
     9241 ttaatctact tagttttata tttgtatggt attactatag agattctaag tatgattttg
     9301 ttagtgataa agtaaatgtg accaatatca agaattagtt gattataata aattttaata
     9361 atatcttcta agcttaaagt attctgtagg attgttggaa atttacaaaa atataaaaat
     9421 aacttggata ttctatttac tccaagttat ttttatattc ttttgtaaat agaaatggaa
     9481 cataaaaaat taaaacaacc atagatacta ttagtatatc atggtaagtg atagttgatt
     9541 ctttagaatt tgtgataaga aaagcactat tatttaaaat atgaattata gcacaaaacc
     9601 agatgttctt agtttttata taaagatatc ctaaaaatat tgaaattcca actatatgta
     9661 tcgaacgtaa tattaatcct aatattggtg tttcaggtgt atataatgta aatattagtg
     9721 gtaagtgcca caaactccaa caaataccta atattataac tcctagtttt ttaccaaatt
     9781 tattaaaaaa aatattctgt aaaaaacctc tccaggcata ttcttcacta aaaaaaatat
     9841 aaatagtagt taatgatgtt attggagtaa atataaattt tatgatacca ctataattag
     9901 gttgagaccc ttgtagtatt aatataaatg atgttggaac aattacagaa atcataaagt
     9961 aaataattaa caaagaacct agtttaatat tttttatagg atataaattt tttttatttg
    10021 atgaatatat tataattga
//
LOCUS       DAMKMI010000141         9864 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMKMI010000141 DAMKMI010000000
VERSION     DAMKMI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.147"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            247..825
                     /locus_tag="PC429_003127"
     CDS             247..825
                     /locus_tag="PC429_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3848609.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            864..1442
                     /locus_tag="PC429_003128"
     CDS             864..1442
                     /locus_tag="PC429_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3848610.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            1528..2157
                     /locus_tag="PC429_003129"
     CDS             1528..2157
                     /locus_tag="PC429_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3848611.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            2147..4906
                     /locus_tag="PC429_003130"
     CDS             2147..4906
                     /locus_tag="PC429_003130"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF3848612.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            4993..6555
                     /locus_tag="PC429_003131"
     CDS             4993..6555
                     /locus_tag="PC429_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF3848613.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            6556..7692
                     /locus_tag="PC429_003132"
     CDS             6556..7692
                     /locus_tag="PC429_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF3848614.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            7756..8832
                     /locus_tag="PC429_003133"
     CDS             7756..8832
                     /locus_tag="PC429_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF3848615.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            8810..>9864
                     /locus_tag="PC429_003134"
     CDS             8810..>9864
                     /locus_tag="PC429_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF3848616.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKS"
BASE COUNT         3940 a         1008 c         1858 g         3058 t
ORIGIN      
        1 ataaaatatg tacttgttta tataaatgat atataattac atattaaaca tagcttttat
       61 gcatttaata aagtatgtaa aatctatgta aaaagtaaga aatataaaca tttttttaat
      121 tatattttaa aaatatcaag caagttttat tcacaaatga ataaaaaatg ttataatatg
      181 attaaagaaa aagataaata attagcaaga tttgtttagc taaaattaac aagggagcga
      241 taaattatgg gtataacatt atcaaaaggc caaaaggttg atttaactaa aggaaatcca
      301 ggtcttaaaa atattttagt aggtttagga tgggatacta acaagtatga tggtggattt
      361 gactttgatt tagatacagc agcattttta acaggagcaa gtggaagtgt tactaatgat
      421 ggagattttg tattttacaa caatttaaaa catacatctg gagcagttga gcatctagga
      481 gataacagaa ctggcgaagg tgatggagat gatgaacaaa tagttgttga tttatctaag
      541 attcctggag aaataagcaa gatttctttt actgttacaa ttcatgatgc aacagaaaga
      601 agacaaaatt ttggacaagt aagtaattct tatataagaa tagtagataa agacacaaat
      661 gaagagttaa taaagtatga attaggagaa gattttagta tagaaacagc tatagtagta
      721 gctgaaatat ataagcacaa tggagaatgg aagtttaatg cactaggttc tggctttgaa
      781 ggtggtcttg ctgcattatg tggaaacttt ggaataaact tataatttca actataacaa
      841 taaactaaag ggggatattt tccatggcta tagtattaaa aaaaggacaa aaaatcgatt
      901 taactaaggg aaatccaggt cttaaaaata taaaacttgg actaggatgg gatacaaatt
      961 catttgatag tggctatgat tatgatttag atgtaagtgt tttcatggtt ggagaatctc
     1021 aaagagttga aaaagatgaa gattttatat tctacaataa cttaaaacat ccatctggag
     1081 cagttgaaca cttaggtgat aatagaactg gtgaaggtga tggagatgat gaggaaatat
     1141 tagtgaattt agagacaatg ccaaagcata tacaaaagat agctgtaaca gttacaatat
     1201 atgaggctgc tgaaagaaga caaaattttg gacaagtaag caactcatat ataagagtat
     1261 taaattctga aaatgatgaa gaaatattaa gatatgactt aggtgaagaa tttagtatag
     1321 aaactgcaat tacagtatgt gagatatata agtataatgg agaatggaag tttagtgcag
     1381 ttggagcagg atttgaaggt ggcttagaag cactttgtag aaactatggc ttaaatgttt
     1441 aatttaagca atataatata ttaaatataa ataacctata caaataatgt ataggttata
     1501 ttatataaaa ttttgtggag gtttagtatg tcgattaatt tatcaaaagg tgataaaata
     1561 gatttaaaaa aatctaaccc aggactgagt aacatacttg taggtctagg atgggaccct
     1621 gtgcaacaat ctggcggagg tttttttaaa tcgttatttg gtggaggaca agctgatata
     1681 gattgtgatg catctgtatt tatgcttaat caagaaggta aattaagtgg aataaaagat
     1741 ttaatttatt ttggaaatct aaaaagtgcc tgtaaatcag ttttgcatac aggagacaat
     1801 ctaactggtg agggtacagg tgatgatgaa caaatcctag ttaatttgga taaagttcca
     1861 tcaaatattc ataaattatt gtttgtagta aatatataca attgtgtaga tagaaaacaa
     1921 cactttggaa tgatagaaaa tgcctatata agagttgaag accaaggtaa taagaaagag
     1981 atagcaaaat acaatctatc tgataattat tctgaaaaaa ctactcttat agtaggagct
     2041 atatatagaa aagatggaag ttggcaattt aaagcaattg gtgaaggaac aaaagatgcg
     2101 ggtcttaaag aagtaatgca aaatttagac aggattgagt gtgcatatgg aatttaataa
     2161 tgatatcaaa tattataatg tagataaaga agaagttaca aaagacttat ctgttaatcc
     2221 tgaaaaaggt ttatcagaat cagaagtcaa gactagaaga gaaaaatatg gtttgaatga
     2281 atttactcca aaagaagagg gtagtttttg ggatgattta aaggaaagtt taagtgaacc
     2341 aatgatagtt atcttgatta ttgcagcagt agtaagtgca gtaattgggg aaactcatga
     2401 tgcaataggg attgtaggtg ctatagcaat aggtatagca ataggtatga ttacagaagg
     2461 tcgttctaag aaagcagcag aggctctttc taagttgact gaaaacatag aagtaaaagt
     2521 attgagagat ggggaagtac atcaaatttc taagagtgaa ttggtaccag gtgatatagt
     2581 atatattgaa actggtgaca tggtacctgc tgatggtaga cttatagaaa gtataaacct
     2641 taaaattaga gaagatatgt taacaggaga atctgatgac gtatctaaaa agtgtgatgt
     2701 agtagtttct atggaagaga tagaatctaa aggagcagta gtagtacagg aaccaatacc
     2761 tgcaaaacag ataaatatgg tgtttggtgg tacacttgta gcttatggta gaggagctct
     2821 agttgtaact tcaattggag ataatagtga aatgggtaaa attgctcaaa acttatcaga
     2881 aacagatgag caaactccac ttcagataaa acttggaaat ttaggtggtt tgatagccaa
     2941 agtttctagt gcagtagcag gattactatt tatatttatg gttttccaaa tgataagtaa
     3001 gaatgtatta aatgtagata tgtctggaat attaccgttt ttagaatcaa tagacccagt
     3061 taaaacagca tttacagtat gtgttgcgtt gatagttgca gcagtgccag aaggtcttcc
     3121 aactatgata aatatgacac ttgcaataac aatgcaaaaa atggctaaaa taaatgcatt
     3181 ggttaccaaa aaagaagctt gtgaaacgat aggttctgtt tctgtaatat gttcagataa
     3241 aactggtaca ttaactgaaa atagaatgac tgtagaagtt gcatatgttg atggcagata
     3301 tattgaaagt tcagaagagg aaataaatag ttattttgaa gaaaactgta tgataaactc
     3361 tacagctgat gtagagcaca atgatggtga tattaagtat ttaggaagtg ctactgaatg
     3421 tgcattgctt ttatattaca aaaacgtaga ttatagacaa gttagaaaaa attctaatat
     3481 agtagctcaa aatcctttta cttcagattc aaagagaatg acttctgtta taggtcaaga
     3541 taatcatcat gtactattat caaaaggagc accagaagtc ttacttgaat tatgttcaca
     3601 tattcaaaga ggaaaagaca tagttccatt aactgaaaat ataaaacatg agattttaga
     3661 agaaataaag aaattgcaaa taaaatcaat gagaacttta gggtttgcat ataaagaaat
     3721 ctcacaagct gaagaagaag cagcagtaac tgctgaaagt gatgctatga atgtaagtgc
     3781 aatggaaaac aatttagtat ttagtggttt tgttggtata agagacccac ttagaaaaga
     3841 tgttatagag tctgtaaata cagcaaataa agctggtgtt tctgtaaaaa tgcttacagg
     3901 agataatata aatacggcaa gagctatagg tgaagagtta ggcctattaa aaaataatat
     3961 gagagtggtt gaagcttcat atatagatac tttagatgat gaagaattaa aacaagagat
     4021 acaaagtatt tcaatcgtgg caagaagtaa accagatagt aaaatgagaa tagtttcagc
     4081 tctgcaaaag agcggagatg ttgttgcagt aacaggtgat ggtataaatg atgctccagc
     4141 actttcaaaa gcagatgttg gtatagcaat gggtatatct ggtacagagg tatctaaaaa
     4201 cgcagcagac ataattttaa ctgatgatag ttttagtacc attgtaaaag gtattaaatg
     4261 gggtagagga atttatgata acttccaaag attcgttcaa ttccaattaa ctgtaaatgt
     4321 aattgcattt ttagtggcta taatatctca agttatggga caagagatgc catttactac
     4381 aatacaatta ttatgggtaa acatcattat ggatggacca ccagcactag cattaggttt
     4441 agagccagtt agggaccatg tgttaaacag aaaacctgtt gatagaaaag ccaatataat
     4501 ttctaagtca atggtgtata caatagtatt aaatgcattt tatataacag caatactaat
     4561 gttacaatct acatttaatt tcttaggagt tgatgaagcg cataagggaa cagtaatgtt
     4621 tgcgttattt gcttttagtg cactgtttaa tgcatttaac tgtagagagt ttaatgcaga
     4681 tagtatcttc cctaatttta cacataacaa attagcacta caaataatag tgttgacagg
     4741 aattgcacaa gtaatattta ctcagctatt ccaagatttc tttaactctg tagcaatgga
     4801 ttttatgaca tgggtaaaag ttttatgtgt agcagcaagt gtaattgtgg ttaatgaagt
     4861 tgtaaaatgt ataataagac tggtaaaacc aaaaaagact gcttaaaaca aattttttag
     4921 aagaaaacag actgactcaa aatatgaatt ttgggtcagt tctgaataaa catttaaatt
     4981 gggaggatag ttatgggtga ctcaaaggta tttgaaaaaa tattctccag ccaatcagat
     5041 agaggaaatt acactccttc aaagggatat ctaagttatt ttatttcgta tattggttta
     5101 gaagatgaag ttttgtacaa tctcgagata tttaaaacaa aacaaaatat tgatagcaaa
     5161 aaagatattg ctttatttac tgatgttata gcaaatccta gtgattttga tattataaat
     5221 tattttaaaa gtggacttca aaaatataga actagcatgg aagatgtaga tataaatatt
     5281 ttgggatttg aagagattga ttataaaata aagcaagcaa tggatagagt gttaaaagaa
     5341 gaggaaaaag aatttactaa tgatagagtt aaacaaaatt ttatagtgaa aataatggca
     5401 tggataaaaa tttatatcgg tgctttagat ataaataaaa atgaagctcc aaaagtaata
     5461 ttttatggag atataaaaaa acatgaagtt tatttactct taatattata cttggcaggt
     5521 tttgatgttt tatatcttaa tccaaattca aagagtaata tagatatttt aaaatcagaa
     5581 agatacaata tagagtttga agaagccaac ataatagaag aaaaaatttc ttttgaagaa
     5641 agggttattt taggggaaaa aatagataaa agttctgtaa aaaaagcttt tactgttggg
     5701 gcagaagcat caaagcgtat aagtgaggaa ttgttaaatg atgcaggatt tataaaacct
     5761 tggcaactcc aagatagaaa aataaaaaat ctacttctaa gttcaactgt tgatgaaata
     5821 tcaatatact ggaatcaacc acttaaatta agaccaggat ttaagtttaa tgatgctatt
     5881 gtagaagctc caaattttct ttctaagatt aatggaatat ataacgataa gaatgaatat
     5941 attaagtttt tagatttatt aagagattcg gaaagttcaa catttattga atttaatgga
     6001 gatgtagata gattttcaaa agcttttaca agagaagctt ttagtttatc ctttttattg
     6061 gattcaaaag gtgcaataga taaaaactct gttttaaata ataaagatta ttccatatca
     6121 acattagcat tgaatcaaca aattatgatt ttagagaaag tggaagagtt gttagaagga
     6181 agtatgtttt tgaatggttt aagtggagaa gacaagataa aaggtctatt tacagtactt
     6241 cacatggata agaaatttgt tcatatgatg aacaatttcg attactcact tataaaccca
     6301 aaattgatta tatacatgta caagagtatt gtatttgata aagaaatagt ttttcttatg
     6361 cttttactta gtaagattgg atttgatatt atcatattat gtccaggagg agaaaacaat
     6421 atagagaatg ttataaataa ccaattgatt gatgtacaca gattggataa aatggtatat
     6481 gaccttaaat taaattcgct tgaaaatgat atacctttac ttaaaaaaat ttttggaaag
     6541 aggagacgat tttagatggg gataaatata aatagttttt caaaagataa gaactctgta
     6601 gaggttactg agaatttgcc tacagcagtt caagaagaaa acttcgatat aatggaatat
     6661 acaaataata aaaaaaatga actgagaaag tctaaggaag ttgaggcatt gacatctctt
     6721 atagaagtag aaaatccaga tacgatttta cagtttggta ggaaagcatc tgaaggagta
     6781 gcaagagttt ctgatagcct tttaaataca ataaaattaa atagaaatga agaaaattct
     6841 aaaatgctgg ttcatttaac taaaataatg gacaagtttg atttagacga ttttcaagaa
     6901 actaaggaac caaatttcgt tcaaaaactt ttcaaaaaag ctaataatgc cattgaaatg
     6961 atgtttcaaa agtatgaaac attgggagga gaagttgaga aaattcaaat tgagcttgaa
     7021 caatacgaaa gagatatagc actttctaat aagcaaatag gtgctatgtt aaatgaaaac
     7081 tttgagtttt acaatgaact tcaaaaatat attgttgctg gagaaatggc aatagaagaa
     7141 atggataatg aaatactacc tttctttaaa caaaaatcag agactagtgg agaccaaatg
     7201 gatgttgtca attatcaaga actattaaaa gtttatgata tgttaaatca aagagtctat
     7261 gatttaagga tagcagaaaa tatagctatc caaacaatac ctatgcttag aggtatgcaa
     7321 cataataatt atggtttaat tagaaaaata aatagtgcat ttgtcgtaac tttaccagta
     7381 tttaaacaat gtttatctca agctatatta cttaaaaagc aagaattaca agcaaaatct
     7441 ctaaaagcac ttgatgacaa aactaatgaa ttattgctta gaaatgcaca aaatgtaagt
     7501 actcaaagtg cacaaatagc tagaatggct ggaacaagtt ctgttcaaat agaaacattg
     7561 gaaaaaatgt acaatactat taaatctggt atagatgaaa ctatgagaat agaagaaaac
     7621 aatagagctt taattaagga taatacaaag agattggaag aattaaatac gactataatt
     7681 tataataagt agtattatat taagggctaa tttgtataaa gttggccctt tctatgtata
     7741 tgaggaggct agacattgaa atatttcgat tatatctgca aagatgattt ggagagaatt
     7801 tttttgaagg agccagaaga ttttagtgct aagactgaaa aagatgtgtt gaaatatgca
     7861 ctgggcgctt ttttatacgt tccagctact caatataata tgatttataa gagtattata
     7921 ggggatgtta agggagttag accattagct atttgtttag aagatgctgt tggagtaaat
     7981 ggagagttag aggctataga aaatcttaga ttaattttaa aaaatattag caatgaaagt
     8041 ataactaata aagatggaat accactaatt tttgtaagaa taaaagatgt ggagcaactg
     8101 ttgagaatta aagagattat aattaaaaat agacattcta taacaggaat attaattcct
     8161 aaagcaaatt cggagttaat agaaaattgt atagaagctt tagattctat gaatcttcaa
     8221 gatatgtatg ttattccgat aatagagaca aaagaattta tatataatga aaaaaaagaa
     8281 cttagtttta ctaatttgta caatgctatt ttaagacata agtcaagaat attaagcatt
     8341 agagtaggca taactgatat attagggatg tatggtataa ggagagataa gaacttctct
     8401 atatataata atttgatttg ttcttcattt atattagata taatcacata tcttcaaaga
     8461 ccagagttag atatacctat aagtggtgga gtatcagaat tttttgatat gacgaataag
     8521 gatataagaa ataagtatat agaagaaata ttattggata aatatcatgg attaattgga
     8581 aagaccgtaa ttcatccaat gcaaattcaa attgtacaag cgctatctac agtatcatat
     8641 gaagatttta ctgatgcttt ggatatactg gatagtacag acagtaagta tggtgtgagc
     8701 aagggtgtat taggagagag aatgaatgaa acaaatccac attttttatg ggctaaaaaa
     8761 acattgattc tatctaaaat atatggtgta ttaaataaag gagtagacta tgaagaattg
     8821 cttaaatttt aattgtgaga ttgacattat tgataatcca ttgaatttag aactaaatga
     8881 ttttttagac gtaggaataa gaaataatag caaaagacgt tttttgttta tctcaaagaa
     8941 actcggtaaa catttagcat gtaagccaag tgagatggat aatttgggaa aattaatggc
     9001 tacaatttat aatgaaaaaa ataaggaaga atcaaatggt actgttatag cttttgctga
     9061 aactggtaca gctgttgggc atagtttttt tgattactta tgtggagatt atgaatttat
     9121 tcatactact agagagcagt ttgacgagtt agaaaatctg gaatttttag aggaacattc
     9181 ccatgcaaca gaccagaatt tatactttgg tatgcttaac aactttaaat ttggagatga
     9241 gataatattg gttgatgatg agattaccac agccaataca tgcatgaata taattaggaa
     9301 aattcaaagc atttatccta aaaagaaata cactatatgc tcaatactca attgggtaag
     9361 tgatgaaaat ttaagtaaag ttactcgttt agaaagagag ctagattgta agatagattt
     9421 tgtatactta tttaaaggaa actttaaatt ttatatagat gaagatatta tttcaaatta
     9481 caacgataat tatagtatta ctaaattaga gactatttca agtgataaag taaatttagt
     9541 agtaaatcat atttatgttg atttagaaga ttatattgga aataagaaat atgtaaaata
     9601 tacaggaaga tttggaatta atagaagaga acaatttaga ttaaaagaaa tcgttaagag
     9661 agaaagtaaa aaaataacta tggccagtaa agaatcttta aaaaatgaaa aagaaaagat
     9721 tctattttta ggtactgaag aatttatgta tatacctatg ctgtttgcaa aagaaaaaga
     9781 aaatcaatat gatatatact atcattcaac aacaagaagt ccaattatta atattgataa
     9841 gaataattat cctattaaaa gtaa
//
LOCUS       DAMKMI010000142         9823 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMKMI010000142 DAMKMI010000000
VERSION     DAMKMI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.148"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            156..1124
                     /locus_tag="PC429_003135"
     CDS             156..1124
                     /locus_tag="PC429_003135"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF3848617.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            complement(1241..2371)
                     /locus_tag="PC429_003136"
     CDS             complement(1241..2371)
                     /locus_tag="PC429_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3848618.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(2524..3477)
                     /gene="msrA"
                     /locus_tag="PC429_003137"
     CDS             complement(2524..3477)
                     /gene="msrA"
                     /locus_tag="PC429_003137"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF3848619.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTHIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            complement(3601..4506)
                     /locus_tag="PC429_003138"
     CDS             complement(3601..4506)
                     /locus_tag="PC429_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF3848620.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            complement(4878..6470)
                     /gene="hcp"
                     /locus_tag="PC429_003139"
                     /gene_synonym="priS"
     CDS             complement(4878..6470)
                     /gene="hcp"
                     /locus_tag="PC429_003139"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF3848621.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDICGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            complement(6530..7255)
                     /locus_tag="PC429_003140"
     CDS             complement(6530..7255)
                     /locus_tag="PC429_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3848622.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSMSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            7420..8124
                     /locus_tag="PC429_003141"
     CDS             7420..8124
                     /locus_tag="PC429_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF3848623.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYTKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            complement(8288..9559)
                     /locus_tag="PC429_003142"
                     /pseudo
     CDS             complement(8288..9559)
                     /locus_tag="PC429_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416360.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
BASE COUNT         3266 a         1662 c         1154 g         3741 t
ORIGIN      
        1 tagagagtat attaacattg cttttttaaa aattttagta taaaatcata ttaaataaat
       61 tttctgaaat aatagaatat taaaatatcc aaaaacagct tttaataaca tctataattt
      121 tcaatgtatc gttattttag ggggagtgat ttaaaatggc aataaagcca agaaaaatat
      181 ctattgtagg ttcagggcat gttggttctc attgtggatt tagtttaata actcaaggag
      241 tttgtgatga actctttatg attgatatag atgaatctaa atcaaaagct caagctttag
      301 atttagctga tgcagtttca tatctgccac ataaagtaca tatagaaaaa ggaacttttt
      361 ctgactgtaa agattctgac atagtagtga taagtgttgc agactcttca gaagggcctt
      421 tacgtagaca aaacacaact agattagatt tactaagacc aactattgga atgataaaat
      481 ctatagtaaa acccatagtt gattctggct ttgatggtat atttgtagtc atatctaatc
      541 ctgttgatgt agttacaaat tatatttggg aaaaatctgg ctttcctaaa aacaaagtca
      601 ttggaactgg aacagcttta gattcaacaa gacttagaag gattttatct gaagaaactg
      661 gaatcgctca acaatcaatt caagcttatt ctatgggtga acatggagat tctcaaatgg
      721 ttccttggtc tcatgtttca attggtggta aaccaatctt agacatgata aaagacaacc
      781 caagtactta tagcaattta gacttaccat caatagttga aaaaaataaa aaaacaggaa
      841 taagtataat aaatggtaaa gattgtacag aatttggtat aggtactgct ttagtagaaa
      901 ttgtaaaagc tatactacac aatgagaaaa aagttcttcc agtttctaca ttactagaag
      961 gtcaatataa cgaaagaaat gtatttgcag gtgttccatg tgttatagga aaagatggta
     1021 ttgaagaaat tattgaaatc aatatgactg aatatgagca aaacgaattt aataaatctt
     1081 gttctgtttt aagggaatat attgcacttt caaaaacact ttagattcaa tattctttct
     1141 tacactaaaa gcaggtaaat gacttattga aaagactttt aactaagtta atgtttaaat
     1201 catttgtacc tgcttttaat tttaatacta aaatataaac ctatttttta ccatcaaatt
     1261 ttataacttt acttttttta tttacctctt gagataacaa tccttttttc aatcccatca
     1321 ataattctaa gtcttctgga taaagattta ttccaaattc ctctaaactt gatactatac
     1381 tatttacact cattatttta ttttctatca ataattcaac tgctcttttt aaatacgttg
     1441 ggcttgaacc tttcattttg tctagtggtt cttctatcaa ccagcctttt ttatccattt
     1501 cattcataag ataattgtat tttttgtaat ttatcttttc tagattatat gctctaaaaa
     1561 tcatagcaga tataggtaca atccattttt tctttagttc tacataataa tctaaatctt
     1621 ctggatgagt caaatctttt aaaaaactct cttttggcaa taaaaatgca caggcatact
     1681 catcttctga aaattgctta cttggtattc ttaattcact tgatattata tatccaagct
     1741 cacatgccaa agtataattt ctaattgatg ctgacttttt gtcattaccc agagatatta
     1801 gatatttaga ttctttacta attctctgtt tttgagtaaa gatagttgcc ccttttctat
     1861 ctacattcat acctgatatt ataatccctt tagactctaa caaacttacc atatttacta
     1921 ttggagtact atttaaatcc caaaactctc tagtcttata tgcaagagat tctatatcat
     1981 ttatattaat atctgacatt acttttctat ttggcaagtt ttcttttgga aacttaatat
     2041 aattctccat aaatgaatat attttatgta ttataattat tttttctcta taagaaatct
     2101 cttctactct aggtagttta gattgagggt taaagtggct atcctctaca acgatattta
     2161 tattttcatt tttataaaaa tactcttttg gaaaatttaa tatattagat aattttaatg
     2221 tattttctat atttggtaca tacttatttt cctcaaaagc ttttaaatct tttttattta
     2281 ttttagtttc tttagacaac tgttctatcg tctttgctct aaaaattcta gctgatttta
     2341 atctctttcc attaaagtct tttaaattca tttgttttct ctctttcaaa attaaattct
     2401 taatctattg gtataaataa gattacaatt aataatttat catattattg acaaaaaaac
     2461 aaaaggtgaa aatatttttt tatattttca ccttttagat aagtttataa taaatattcc
     2521 taattactca aaaagtttta aatattctcc atatcctaaa gcttccatat tttctcttgg
     2581 tataaattta agagaagcag aatttataca atatcttaaa cctccaagtt cttttggacc
     2641 atcatcaaat acatgaccta aatgagaatt tcctttagca cttcttactt caactctgta
     2701 cataccttgt gagaaatcac ttctctcaat gatagattta tctataactg gctttgaaaa
     2761 acttggccac ccacatccag aatcaaattt atcttttgaa gaaaataaca cttctccact
     2821 tacaatatca acataaattc cctctttgtt attgtcataa tattcatttt caaaaggtac
     2881 ttctgtcata tcattttgag tgacttcaaa ttgtatatga gttaaagtct tttttaactc
     2941 ttccctattt atattgtttc catcccaatg gctttttaca aaattatatc ttccagaccc
     3001 tttataatac atattgtagt ttaatggtct tttcttataa taatcctgat gattttcttc
     3061 tgcttcgtag aatttagttg ctggaattat atcagtcact attttactat caaatatttt
     3121 actattttcc agatgttctc ttgactctaa agctatattt ttttgttctt catcatgata
     3181 aaatatagca gtcttatatt gagttcccct atcaccaaat tgtccaccat catctgtagg
     3241 attaatctgt ttccagaaga tatttagaag ttcatcataa cttattatat catcatcata
     3301 tgttatttga attgcttcta aatgaccagt agttcctgag catacctctt gatatgtagg
     3361 gttttcaaca tgaccacctg tatatccaga aactacttta ataactccat catacttatc
     3421 aaaaggtttt accatacacc aaaaacatcc acctgcaaaa gtagcaagtt ttatcatttt
     3481 catcactcct taaataaatt atactttatt tattccctaa gaagtaactt aataatctgc
     3541 attttattat ttttacctac tcaatgaaat catgtgtaaa aatacaattg caaattgaat
     3601 ctaatttcta taaaagctct ttttctttat ccatccaaaa gcatctttta tatcacaatt
     3661 ttctcctaaa gacattagat attctaatgc atcacaatca tccatagcta catctttttc
     3721 catcttagga tttaatctat agtaattatc tccaagaagt ttttgagaaa ataactgact
     3781 catttgagaa tttccttcta aggtaataga tataattggt aaatcgggtt ctttattaat
     3841 tatccagtca atagctcccc attcagtagt atctgccttt atactgttat atacatatcc
     3901 agtccctatt gaaaaaagtc ttgtattctt aagtttaaat ccactgtcaa tggcatgaat
     3961 tatacacgct aaactcgggt cagttgctat tattcctcca tcaacatgac gattatatgt
     4021 tggaaaaaat accggagcag cactacttgc cattgctaca tctacaactt tgctagtctt
     4081 tgtaaaagaa tttggcatat tgttataaaa cactggtttc catgcatttt cctcatttcc
     4141 tatataaaaa cttggtatca ttactaattt atttaaatct tttaattcta gttcctctgg
     4201 aaatatactt aataaaactt cttttaagtt tttgttttca tactttggtc tgagtatttc
     4261 tgcataactt ttattaaaaa tatactttga attttcaatt gagtaaagtt cttttatctc
     4321 tttagggctt acaccatacg caagccctag tgcaattaga ctccctgtag atgtacctcc
     4381 aaacatattt atattgttta atagatttgg atactctttt actatcctct caagtatgga
     4441 tatactcaat gcccctttta atccaccacc atcaaatgcc aatatattaa aattagaatt
     4501 tttcatatac atataccccc acataaaaca ccttatattt taaattttaa tcaaaataat
     4561 ttcactatag tatatatatt ttatgcaagt tacatattac aatgttcctc ttttaatatt
     4621 taagttacat tcaaaccttt aatacttaaa ataaaatata taagatatta tatatctaag
     4681 tattataaat ttaatacaaa gattcttaaa aagaatatac tgaaagcagg tttatttttc
     4741 aattattaca aaaaataaat aaagggtcat ctcaaaattg attttttaat caaattttaa
     4801 gtttaacccc ttatcttaat tgtggcatac ttaaaaatat tttaactata aatatcttta
     4861 agtatagata tgtttattta tcctaaaatc ttcttcatat cttcttcagg agtagatata
     4921 ggtgtcaatg caaatttatc aaccaatata tctaatacat tcttagataa gaatgctggc
     4981 aaagaaggcc ctatatagat atttttgata cctaaatgaa gtagtgtaag aagtatacaa
     5041 acagctttct gctcatacca tgacagtacc atgctaagag gcaaatcatt tactccacat
     5101 tcaaaagcat ttgccaatga aactgccact ttaattgcac tataagcatc attacattgt
     5161 cccatatcca ttaaacgagg taagccacat atatccccaa ggtctaaatc attaaatcta
     5221 tattttccac atgcaagtgt caatataaca gtgtctttag gagcttgttt tacaaattca
     5281 gtgtaataat ttcttccagg ttttgcacca tcacaacctc caaccaataa aaagtgtctt
     5341 atagctccac tctttacagc atctactact ttatcttgta cagatgaaac tgtacctctt
     5401 gcaaaaccag tagttaatgt atctcctcca ttaataccag tcatatgaac atcttctcta
     5461 tatcctccaa gttcaagagc tttttctatt acactagaaa aatctttatc ttcatcaata
     5521 tgaaccatac ctggataagc aactacttct gtagtaaata ctctatctct ataactatct
     5581 ttaacaggca ttaaacagtt tgttgtaaat aatattggag caggaatatt atcaaattct
     5641 ttttgctggt tttgccatgc tgtaccaaaa ttacctttta gatgtttata ttcttttagt
     5701 tttggatatg catgtgctgg taataactca ccatgagtat atatatttat acccttatct
     5761 ttcgtttgtt ctaataattt gtgtaaatca taaaggtcat gtcctgtaac tacaataaat
     5821 ggccctttct cgattttcat agatacctga gtcggttctg gtgttccata agtttcagta
     5881 tttgctctat caagcaattc catacatttt aagtttattt ctccagtttt cataactaga
     5941 cctaaaagtt cgtcaataga caagtctcct ccaactgcta ttaatccttc atagaaaaat
     6001 tcatttacgt ctttgtctgt ataaccaagt accattgcat gatatgcata ggcagccata
     6061 cctcttaatc caaataagat taatgatttt aatgaacgta tgtcctcatt atcattccat
     6121 aaatccttca tatcatagtt atcatttcta ttacaagttg ttgaacaatc tgaacatcta
     6181 ggtacaagct tacttttttc tacctcagtt ttttcaattt gtctttttat agcttcatca
     6241 tcaaaactta cattcgttat agtagtgaat agcccctcta tcataatttt atctgtttca
     6301 cttgttggtc tattcttatt tccaatagtt gctcttgaaa gaccaatcaa cactcctgta
     6361 agttcgtctt gtaagttagc tgtacccccc tttttaccac agacaccatt tatagtacaa
     6421 cctttacctc ctgctgtttg ttcacattga aaacaaaaca ttttttccat tttttattcc
     6481 tcccttttta tttattaaaa tttattatat ttttctataa ttcacttaat taaatttctc
     6541 catctacaat ttttccatct acagatatag taactatttg ccatggtatc atttttccac
     6601 tattttgtag agctgtctta acagcattta ctataccacc acagcaagga acttccatac
     6661 gtgttactac tatattttta atatcatttt cttttaaaat ctctgtaagt ttttcagcat
     6721 agtcaacttc atcaagttta gggcatccga ttaatgtaac cttatttttc ataaatttat
     6781 tatgaaaatt tccatatgca tacgctgtac agtctgctgc tattaaaaga cttgcattct
     6841 ttaaatatga tgcattagtt ggtactaatt ttatttgtac tggccattga ttaagttgag
     6901 aaccttttat ctctactata tcattgctta tagacatact atttgaaacc tctctattta
     6961 ttgacttaga ctgagaacct ggacatccac atgctaaagt tttcttttgt gctttctttg
     7021 cttccatatt tgcttttact gcagcttcat catattctgc tgcttctcta tactcaaatg
     7081 aaatcgcatt tgttggacaa gttggcagac aatctcccag cccatcacaa tagtcatctc
     7141 ttaaaagttt tgcttttcca tttaccattc ctatagcttc ttcatgacaa gcttcaacac
     7201 atagtccaca accattacac ttttcctcat caattttaat tatttttctc atcatttata
     7261 tcacctcata aaattttttt aatttaaagt attgatttct tatatatata tttttaatac
     7321 ctatatttct ttatttcaaa tttcttgtag taggattttt atatcccctt gcttttataa
     7381 acttagtata gtacaatgaa aacaaattat cggttgcata tgcaacaagg aagtgattat
     7441 ttgaaaaaat ttttagaaaa gcatatagac ctacttaaaa aaattcctct atttgaagga
     7501 attaaaagtg atgaacttga agaaatgttg gaatgtttag gtgttgtaga taagacttac
     7561 acaaaagata gtataatatt ctctgttgga agtgaagtta cttctattgg aattatgctt
     7621 aaaggaagtg ctcatataat aaaggaagat atcgaaggga ataggaatat tgtagctgaa
     7681 ctcttgcctg gagatttatt tggagaggta ttcgcatgta ctagactaca taaaagtaat
     7741 gttacagtaa ccacaacatc ttcttgcgaa gttttattca taaagtttaa aagtgtaact
     7801 ggtatttgtt catctgcatg tgtatttcat aataggcttg ttgaaaatat gctacaatta
     7861 atagctgaaa aaaacatttt acttcacaat aaaatagaat tactttctcg taaaacaact
     7921 cgcgagaaat tgcttatgta tttttcaaaa cagatagaac aaacaggtag ccaccagttt
     7981 acaattccct tttcaagaaa tgaaatggct gattttttat gtgttgacag aagttctatg
     8041 tcaagagagc ttggcaagat gcgagatgaa ggcttgttat cttttaacaa aaataaattt
     8101 gaaatctttt attttaaaaa ataaaataga tatttaaaca tatttatttc aatatatact
     8161 atattagttg attcaaatta taaatatcat atttgaatct taaatttata tttttactta
     8221 aaaagttaat aggtatctaa tttattaaaa ttaagatacc tattaacttt attaattaag
     8281 tgtacaatta gcttattttc tttgtagtaa ttgctgtttt atctataagc caatcttttc
     8341 cttctactaa tcttgaaact aacattgatg atactgtatt tccagttgta tttaatatag
     8401 tattaggaat atcagttata gtagttataa ctgttaacag tggaattgcc tccactggaa
     8461 aaccgaagat gctaaccacg agcatctcac caataagtca gccacctgct acaggagcca
     8521 taaatactcc aacaacaaat acagttataa ataattgacc tatcgttgct ggatttgcca
     8581 ttccactacc taaaatacct gataaaaatg ccacttttat tattactgct aaaactgacc
     8641 catctttatg tatatttatt ccaagaggta taactgtttc tgatatatca tcaggaactc
     8701 ctatattttt actagcttct aaatttgatg gtatacatgc tgaacttgaa gaagtagcta
     8761 gtgccgttgc tattggtttt atagcatttc tccagaattg tctaaaccct tgttttcctg
     8821 cagataaaaa agcatacaga ctgtaaaata ataagaaata catggctgct gttagtgaat
     8881 aaattacaaa cacccttaca tatccttgaa ttattttact tccaagttcc ccaattattg
     8941 tagctgtata acagcctagc cctataggag cataaagcat gaatatactt actactttta
     9001 atattgcttc cgttccttct gataaaagct ttcttattgt atttccccta tctcccatca
     9061 tagctattgc tgttccaatt aaaactgcaa atataactaa ttgtagtaaa ttactactgg
     9121 aaaataaatc tccaaaattt ttaactgtaa tacttgatac tacttggtct aataacgttc
     9181 cttttgattc taaagtttct gcatcttgag tcaccattaa attttttaca cttgatacat
     9241 ctaatccttt agacggatta aataataatg cagatgtaat acctaaaaat gcaattacaa
     9301 gtgttgttcc cataaatact atagctgtac ccttcattat ttttccaaat cgttccatat
     9361 tttttatact agcaacacct gaagaaacac taagaaaaac taatggtact actatcatta
     9421 atagagcatt tatgaataaa tctccaaatg gttttagtat tgaagctttt ggaccaagta
     9481 ctaatcctac tatcgcccct actatagtac atgctaaaag tataattgat gacttataat
     9541 tttttaaaaa gtccctcatt tatattttcc tttctaatgc tttatattat aaattacccc
     9601 aaatgctaaa aaaattaaag taaattatct aatgcgcatt aataattttt gtaattttcc
     9661 ccaacagcta ctttagcaaa tactggaata tatgtcaatt tctatagttt ttttgtattt
     9721 ttaacgtttt attaatagtt catatctata tataaatatt aataaaattc tgcttttaaa
     9781 actttttttc tagattaatt ttaataaaac aaacaatata aaa
//
LOCUS       DAMKMI010000143         9771 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMKMI010000143 DAMKMI010000000
VERSION     DAMKMI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.149"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            482..1390
                     /gene="trxB"
                     /locus_tag="PC429_003143"
     CDS             482..1390
                     /gene="trxB"
                     /locus_tag="PC429_003143"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF3848624.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1480..3303)
                     /gene="typA"
                     /locus_tag="PC429_003144"
     CDS             complement(1480..3303)
                     /gene="typA"
                     /locus_tag="PC429_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF3848625.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3515..3757
                     /locus_tag="PC429_003145"
     CDS             3515..3757
                     /locus_tag="PC429_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848626.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3905..4096
                     /locus_tag="PC429_003146"
     CDS             3905..4096
                     /locus_tag="PC429_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF3848627.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            4387..6888
                     /locus_tag="PC429_003147"
     CDS             4387..6888
                     /locus_tag="PC429_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF3848628.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQNVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            7048..7716
                     /locus_tag="PC429_003148"
     CDS             7048..7716
                     /locus_tag="PC429_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848629.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            7700..8977
                     /locus_tag="PC429_003149"
     CDS             7700..8977
                     /locus_tag="PC429_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF3848630.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            9378..9599
                     /locus_tag="PC429_003150"
     CDS             9378..9599
                     /locus_tag="PC429_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848631.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         3889 a         1107 c         1654 g         3121 t
ORIGIN      
        1 aaatagtgct aatatttaaa ctattaatca taattggttc aacaaaagta tatagttaat
       61 aatgtttaat ttgaagaaat tgttagtgtg aaattacata tatattcaaa ttaaattcat
      121 aaatatttca cataataata gtatactgta aacaaaagga ggtattaaat atgggaatag
      181 tacttataac agcagcatta gttttggtta tgtatggact ttcaattcta tcatatatat
      241 cagaaagcaa tatattagaa gacactgtaa atgataacca ataaggtttt gaattatata
      301 aataagtgag aactgtaaat aaaagttaat ttttatttgc agttttttta tatatatgta
      361 aaataaagtt aattaataaa aaataatgtt ttgtttatag tatatatgga taataatagt
      421 aaagtataag aatgatgaga ataatatata agaaataata atataaagag aggtaaacgt
      481 tatggtagat atcattgtaa ttggtgctgg accagcaggt ttaacttcag caatttatgc
      541 tatgagagca gggttaagcg ttacagtatt tgaaaaaaat atttatggag gtcaagttgc
      601 aagtacctct gaggtagaaa attatccagc agttcaaaag atttctgggg tagaattttc
      661 taataatata tacaatcaag cagttgctca aggtgttgat attcagtttg atgaggttga
      721 agagataaat ttagagggaa aagtaaaggt tgtaaaaact tcttcaggtg aacataaagc
      781 aaaggctgtt attcttgcaa atggagttga aagacgtaaa ttaggttgtg caggggaaca
      841 agaatttaca ggtagaggag tatcttactg tgcaacatgt gatggagcct tttttaaaga
      901 taaagaagtt gctatagtag gtggaggaaa tactgcacta gaagatgctt tatttttagc
      961 taataattgt acaaaagtgt atttaattca cagaagagat agttttagag gtgaagaagt
     1021 gttggagaaa tctgtgaaag caagagagaa tattgagata ctttacagtc atggtgtaga
     1081 aaaaatagaa ggagaaaaga ctgtatcgaa aatagaagta aaaaacttaa agactgaaga
     1141 aaaaagaact atagatgtta gtggtatttt tattgctata ggtcttaaac ctaataataa
     1201 aatgtttgaa aatgttcttg atttggatga aggtggatat ataatttcag atgagtcttg
     1261 tacaacatca gtagaaggtg tatatgtagc aggagatagt agaactaagt ttttacgtca
     1321 aataataaca gcagcatcag atggagctat tgcagccgtt caagcagcta attatataaa
     1381 tgttgaataa tataaatttc aaataaaaat agccatataa actgaaataa tttttcagta
     1441 ctatatggct attttgttaa ttagaggtaa atgtacgttt tatttacctt gtctagattt
     1501 gttatatctt aatctatcat gttcatttaa gaatcttttt ctaagtctta tagaatcagg
     1561 tgtaatttca actaattcat catcttctat aaactctaga gcttcttcaa gagtaaatat
     1621 tctaggagga gataatttta tagcatcatc tgaaccagaa gcacgtacgt tactcatttt
     1681 cttgttttta caaggattga ctaccatatc atcttttctg gaattcatac caattatcat
     1741 accttcgtaa acttctacac cagggtctac aaacattaca gctctatcac ttaaggcatt
     1801 aagagaatac cccatagtaa caccaggtcc ttgtgctatt aaaacaccat ttcctcttga
     1861 aggtatttct cctttaaatt cttcaaattt ttcaaatgaa cgaactaaag taccttctcc
     1921 acgagtagcg tttataaatt cacttcgata tcctaaaaga ccacgagtag gtgctaagaa
     1981 ctctattttt acatagtctc cttctattga catagactcc atcatacctt ttctcatgtt
     2041 taattcattt ataatagtac cagagtaaac ttcaggacag tttacaacaa ctctttctat
     2101 tggttccatt agttttccat cttctttatg cattaaaact tcaggtttag aaacaccaac
     2161 ttcataacct tcacgtctca tattttcaag tagtatagat aagtgaagct ctccacgtcc
     2221 agaaactttg tatccatcag tagtatcaag aggttcaact ttaagaccaa catttacttc
     2281 aagttctttt tcaagtctat ctttcaaatg tctagttgta acgaattttc cactttttcc
     2341 aacgaatgga gagtcgttta ctaagaagtt catagaaaga gtaggctctt ctatttttat
     2401 catttccata ggtagtggac tatctaaatc gcatatagtt tcacctatag atatatcagg
     2461 aataccagct ataacaacta tttctccact agtagcttca tcaacttcaa cttgttttaa
     2521 accttcatac acagaaagtt ttgatacttt tcctttagat acaacagaat cttctctaca
     2581 tatagcaact tgtgtattgt ttttaagagt acctttgtaa attctaccta taccaagtct
     2641 acctacataa tcatcatatg caagagctga tatttggaac tgtagaggtt cattatcgta
     2701 attaggataa gcttcaacgt gatttacaat agttttaaat agaggagaaa ggtcttcact
     2761 atcatcatcc atttcaagtt tagcaatacc ttgtttagca ataccatata ttataggaaa
     2821 ttcgcattgc tcatctgttg catttaagtc tacaaataaa tcgaaaactt cattaactac
     2881 ttcttccgct ctctggtctt ttttatcaat cttgtttata aataatattg gttttaaacc
     2941 gaactctaaa gatttttgta aaacgaatct agtttgaggc attgggcctt cactagcatc
     3001 aactaataga ataactgtgt caacagtttt cataacacgt tcaacttcag aagaaaagtc
     3061 actgtgtcct ggagtatcaa ctatgtttat tttgtaatct tcatagttta ttgcacagtt
     3121 ttttgaatat atagttatgc ctctttcttt ttcaaggtcg ttactgtcca taacgcagtc
     3181 tttaactact tcgttctttc taaaaacacc gctttgagaa aggaaagcat caaccaatgt
     3241 tgattttcca gcgtcaacgt gggctataac tgcgatattt ataattttat gtttttgtga
     3301 catttttgtt gctcccttcg attaaaataa cttattaatt atagcacaat atattattag
     3361 gtacaaggag gaatatatta aattgtatta atatagttta aattggataa aactgttagt
     3421 tttgcatctg aaaattattc tgtttaaaaa aattttttag acaaataata accttaatag
     3481 ataaaaagat taataaaaga gtaaaggatg atatatgttg gaagtaacca aaataataat
     3541 taaatatttt gcaagtgaaa gtgatacttt tgtaaaagaa gaagtttgta attttgaaga
     3601 acttcttaag tggtctcata aatttattga aggtaaaaag atagaagatg ttttgtatat
     3661 aaagtctaaa gaaaactata aagtaaatgt gattaagtct ttagaaatta aggaattgga
     3721 aatagtgaga aaacaacaga taccaccgta catgtagtat taataatata taaaattaat
     3781 atgtaatact cgcaatagga tataaatggt atatatggta ataatttata ttaaaaaaac
     3841 ttttataaag tttatatttg ggtataaaag tattaattag acattaatta ggaggaactt
     3901 aaatatgata agagtatgtc caaactgttc aaatgtggat gtaaataagc taaaaacttt
     3961 ggttggagaa gaaaatgtaa agactggttg tataggtcag tgtagagcat ttaaaaaaga
     4021 agctgttgga ataatagaca atgagcttga aataaaggaa acagaagcag agttttttga
     4081 agcatgtaaa gaataaattt taaataaaat ataaattata ataaatagag catctaaatt
     4141 gtataagcat taaacaattt agatgctcta tttttatata tacatttaat tatgagtata
     4201 cttttctatt aaatacaaat aaatataact aatacattta tttattaaaa aaatattgat
     4261 ttaatatacc tatagggggt ataatgaaaa tatagatttc taaataagta atttatcaat
     4321 attacatatc aaaataaaac gttacaaatt agctgaaaat atattgaaag aaaggaagat
     4381 tttattatga gttcaaataa agtggtagaa agttataaaa ttacaggtat gacatgtgca
     4441 gcttgtgcaa aggcagtaga gagagtaact aagaagatgg atggagtata tgaccaaaat
     4501 gtaaatatag ctactgaaaa acttaaaata gaatatgata attctaaggt taattttgat
     4561 gatataaagc aggttgtaga aaaagcagga tatggaatta taaaggaaga aagtaataaa
     4621 aaaattgata tgaaaattga tggaatgaca tgtgcagctt gtgcaaaagc tgtagagaga
     4681 gtggtaaaaa aattggatgg agtagaaagt attagtgtga atattgcaac agataaagct
     4741 aatatagact atgacccatc aaaagtaaaa ttatctcaaa taaaagcagc tattgaaaaa
     4801 gctggatata aaccaataga agaggtaaga aataaggttg atgtggatga agataaattg
     4861 agaaaagaaa gagaaatgaa gtctttattt gttaaattta tagttgcaat agtatttgca
     4921 gtacctctgt tttatatagc aatgggacct atgataataa agcctatagg gccatggcct
     4981 ttgccagaaa ttataaatcc tatgacaaat acttttaatt atgcattaat tcagttaatt
     5041 ttagtaatac cagtaatgat agctggttat aaattttata taaatgggtt taaatcactt
     5101 ttttctttaa gccctaatat ggattcttta gttgcaatag gaacattagc cgcattttta
     5161 tatagtctat atacgacact acaaatagcc aatggacaaa ttcaaggtat gcaccatcat
     5221 cagttgtatt atgaaagtgc aggaataata atagcactta tcttacttgg taaatattta
     5281 gaatcaaaat caaaaggaaa aacatcagaa gcaattaaaa agcttatggg gctacaacct
     5341 aaaactgcaa tagtgttagt tgatggtaaa gaagttgaaa ctcctataga agaagtagag
     5401 ataggagata tactattagt aaaaccagga actaaaatac ccgtggatgg agttgtaata
     5461 gaaggatata catcagttga tgaatcaatg cttacaggtg aaagtatacc agtagaaaag
     5521 aatgttggaa gtaaggtgac aggagctagt attaataaaa atggtgttat taagtttaag
     5581 gctgaaaaaa ttggtggaga tactgctctt gcccaaataa taaagttagt tgaagatgca
     5641 caaggtacaa aagcacctat tgctaaactt gccgatactg tatctggata ttttgtacca
     5701 atagtaattg caatagctgt agttgcatca ttattgtggt ttttaatagg aggaaaagat
     5761 atagtatttg tacttactat atttatatca gtacttgtaa ttgcttgtcc ttgtgcatta
     5821 ggattagcaa caccaactgc tataatggtt gggactggaa agggcgcaga aaatggaatc
     5881 ttgataaaag gtggagaggc actagaatct gcacataaag taaatacagt gatatttgat
     5941 aagacaggta ctataacaga aggaaagcca aaagtaacag atatagtatt aaataataat
     6001 gtaaaagaag aatatcttat caagattgct tcaagtgcag aaaaaggttc agaacatcct
     6061 cttggagaag caatagttaa gtatggagaa gagaagaata taaaatttga aaaagttgat
     6121 aattttaaag ctataccagg tgctggtata caagttacta ttaatgatga aagtatatta
     6181 cttggaaata gaaagcttat gaatgacaat aatataaagc ttggagattt agaagaaaaa
     6241 tcaaatatat tggcaagcca aggtaaaaca cctatgtata tagctgtaga tggaaattta
     6301 tcaggtataa tagcagttgc agatgtagtc aaagaaagta gtaaaaaagc aatagaaata
     6361 cttcatgata tgggaataaa agttgcaatg gtaactggag ataatgcgaa aactgcaaat
     6421 gctatagcaa atcaagtcgg tatagatatg gttttggcag aggttttacc agaagataag
     6481 tcaaaagaag ttgaaaaact acaaaatcaa ggaaagtttg ttgcaatggt aggggatgga
     6541 ataaatgatg ccccagctct tgcaaaagca gatataggga tagcaatagg aagtggaacg
     6601 gatgtagcca ttgaatctgc ggatatagta cttatgaaga gtgatttaat tgatgttcca
     6661 acagcaatta aacttagtca tgaaactata aaaaatataa aacaaaacct attttgggca
     6721 tttggatata atacaattgg tatacctgta gcagcaggaa tattatatgt atttggagga
     6781 cctttattaa acccaatgat tgcagcagca gcaatgagct taagttcagt ttcagtcgta
     6841 tcaaatgcac ttagacttaa aaacttcaag gcatataaga gagattagat taataaatta
     6901 aataaaatgt atccaaaaga gctatcttta aattaaaagg attaatttac aaattaagat
     6961 agctcttttt tcatgtaaaa ttcacaacaa taagataaaa tataagtaat atacgaaatt
     7021 ttaaataaaa tagaaaggat tgatagaatg aattcgtcaa tacttgtaat tgaagatgat
     7081 tcaaatatac aagagttaat atcagaattt ttaagtgcag aaggttatca agttgataca
     7141 gcaaatgatg ggttggaagg tatacaaaag tttaagcaag gaagttatga tttagtaata
     7201 ttagatataa tgatgccaaa cttagatggt tatggagttt gtaaaatgat aagaaagtca
     7261 tcaagcgttc caattatatt tttaactgct ttaaatgatg aaggagacca acttaaaggg
     7321 tttgacttag aatgtgatga ttatataaca aaaccatttt catttaatct tcttataaaa
     7381 agagtagaag caattttaag gcgaagtaac aagactataa atgacaaatt tatagttttt
     7441 gaaaaattaa aattagattt aaacacatat attgcagaga tagatggaga acctatagaa
     7501 cttacactaa aagagtttaa tatattaaaa gctttgatag aaaagtatcc acaagttata
     7561 actagggaag gtcttttgga tagtatatgg ggttatgact attatggaga tactaggatt
     7621 gttgatgcac atataaaaaa tataagaaaa aaaatatcat taccatatat taaaactgta
     7681 aaaggaatag gatatacact tgagaaggat atttgataag tgggaaaagt taagtataaa
     7741 gtataagtta ttttcaataa cgacatcatt attaatagca ttagctttaa taatatatct
     7801 aattttatat tttttattgc catcatatta tcatgagtat aaaattgagt ctcttcaaga
     7861 aagtttgaaa tctttagtag atagttcaat tcatttcgat acatatactt tagaagaaag
     7921 actttattat atggctaaag accaaaattt ggctatttta ttaaaagata accaaggtaa
     7981 aattgtatat ggtaagaatg aagtagttat tttaagatat agtaagtaca tgattaatag
     8041 tcttgaggat gagtatagaa cttctatacc tatatatact aaagatgcaa aagatggtcc
     8101 atatacttta gaactcgtta tgccattaca accaattgac gaagcaaacg aagttataag
     8161 aaaactaatg ccatacatca tatcaatagc aatattgata gcgattattg gagcatatat
     8221 atattcaatt gtaataacaa agcctcttat aaacataata gagagtgaga gagaacagga
     8281 atatagaagg aaggattttg ttgctacaat atcacatgaa ctaaaaactc caataaccat
     8341 aataagtggt caaattgaag gtatgattta tagtgtaggt aagtataagg atagggatac
     8401 ttatctgaaa aaatcgtatg agtgtacaca agaattaaaa gatttagtaa atgaaatgat
     8461 agaggtttca aaatcagaaa tacttgaaaa agatttgaaa ttggtatcaa taaatataag
     8521 tgaattatta aatagacttg taaagagaca agtattttta atagaagaaa aacatatgaa
     8581 aactatttta aaaatagaag aaaatttgga aattaaagca gaccaagaga gaataactaa
     8641 ggcaatcaat aatataataa ataatgccat aaaatattct cctgaagaat cagagcttat
     8701 aattagactc tatgataaaa ataaacgaat aagtaaaaag acttctaatc aaagagtggt
     8761 tttagaaatt gaaaatactg gtgtaactat cgagaaacgt tatttagaag aaatatttaa
     8821 tccattttat agaatagaaa aatctcgttc aagaaaaact ggaggaagtg gacttggact
     8881 ttatatagtc agccaaattt ttaaaagtca tgggtttgat tatagcataa aaaacaaaga
     8941 aaactctgta gtattcacag tagaatttaa aaattaatta cttattataa agaatatata
     9001 actttcaata atagttcatt ttaaaatcac aaaaaattca taatgaaact gtattatata
     9061 aatataaaga agcttaaaac cataaaatac accaactata atgatgagat ttccttacat
     9121 cctccatata agtcatcatt atagtaaccc aacaattaag ttactatgaa tttaaaaaat
     9181 aacaccgagc atataatcta tgcttagtgt tatttttatt ttgaaataaa aaatatttaa
     9241 tttattgaaa tgtatagatt tagatagttt aaactatatt taagacttaa aaattataat
     9301 tgatattaca aataaatata tgtactttgg gaaaaataaa tataggaagt attatattta
     9361 cagaggaggt agttttgatg gatagattaa aaagagatat gaaaaatatg aaagaagatt
     9421 taaaagaagg tgttaaaaag acagcttatg ttatgaaaaa tacagctgaa gatttgaaag
     9481 atgatgcaca aggtgctatg gtaaacttag aaatgaaaaa agatgagatg atggaaagat
     9541 atgaacaaaa gaaattttct aaagaattag agaagcaaat gaagaatgaa atgaaataag
     9601 cttttattta tgaataacaa attattgtat tcatataaaa agtagccagt aatattaaat
     9661 agtttattta aggaaataga ctttctaaaa ataactgtat tagatttaga aagtctattt
     9721 caaattagtt ttataaatag acataaggat aaagtagatg ttatagaact a
//
LOCUS       DAMKMI010000144         9499 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMKMI010000144 DAMKMI010000000
VERSION     DAMKMI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.150"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(161..814)
                     /locus_tag="PC429_003151"
     CDS             complement(161..814)
                     /locus_tag="PC429_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3848632.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPIAEMKKEKLL"
     gene            complement(1059..2924)
                     /locus_tag="PC429_003152"
     CDS             complement(1059..2924)
                     /locus_tag="PC429_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF3848633.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2929..5349)
                     /locus_tag="PC429_003153"
     CDS             complement(2929..5349)
                     /locus_tag="PC429_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF3848634.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     TEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5363..6805)
                     /gene="glgA"
                     /locus_tag="PC429_003154"
     CDS             complement(5363..6805)
                     /gene="glgA"
                     /locus_tag="PC429_003154"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF3848635.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6834..7964)
                     /gene="glgD"
                     /locus_tag="PC429_003155"
     CDS             complement(6834..7964)
                     /gene="glgD"
                     /locus_tag="PC429_003155"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF3848636.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7966..9126)
                     /locus_tag="PC429_003156"
     CDS             complement(7966..9126)
                     /locus_tag="PC429_003156"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF3848637.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         3010 a         1723 c          923 g         3843 t
ORIGIN      
        1 aattctataa gttcattgat ttatatatgt taatttaaat acattattct atcgtagtgc
       61 cttagagttt ttatttgtat ttaaagttaa atttattctt gtttttcctt taatatttaa
      121 tagtaaaaca acaaaattat aaaaaaaata aagagaaact ttataaaagc ttctcttttt
      181 tcatctctgc tataggtatc gttttccaat gttctctcaa aattctttca acattttcta
      241 aatcattctc cctcatagat tttactactt caatatgttg attattgctg tatgtcaaca
      301 ttttatataa ctcatcttca ttatcactag tatatgactt tgacataaaa ttatatctta
      361 aagagttcaa tagatttact aacacctgat tatctgattt agttatgtat atatcgtgaa
      421 attcattact taattttaaa tagctattgt aatttttata ctttatagag accgccatca
      481 tttctgttaa ttcttccatt tttactaggt ctgattcagt taaattttgt aatgctgaag
      541 aaccggctaa cgcatccaat actccaatta cggaataggt gtctaatttc tccttttcct
      601 gaaattcttt tactacaaat cccttattag gtattttttc tagtaaattt tcacctgcta
      661 acctagttaa tgcttctctc gctggtgttc tactaactcc gatagattta caaatctcag
      721 attcagtaat tctctgatta gcttcatatt ctccattttg gattttcttt gatatatagt
      781 catatacttg agttgtaagt ggatctacct tcataagacg ttcctccata ttaagattat
      841 aaaagttaaa tctccctaca actttggtag agagttcagt tttatcttac taatttatat
      901 aatatcacaa ttatgatatt ttttatactt ctttgtaata ttttgaaata ctaaaattct
      961 tatgataatt tatacaaaaa aagtagaaaa agttttgata aaacaaaact ctttccacat
     1021 tcgccttatt ttctatttat ttttctaaaa aaagatattt aatttactct aaaaattcta
     1081 aatgatttag agtctaaatc tactttcaac tcattatttt caatttgtat agtactattt
     1141 gcatttttaa gactatactt tataggtatt tcctcaataa aactactctc tatacataat
     1201 gatatacttt ccacattttc acttctattt actaaaatta acattctatc attattttca
     1261 ttaactctaa taaaagaaaa tacagaatca ttattagtat atataaattc tgtatttcca
     1321 gaacttaaaa gtttattctt atttctaatt tttataacat ctctataaaa attaagcata
     1381 tcttcgtctt catttttcca tggataagtt cttctattat ctggatcaac acctccacaa
     1441 agacctgctt catctccata atatatatat ggtactcctt caaatgtcat ctgtatagct
     1501 acagcaagct tcactaaatc tacatcatca tttaattccg ttttagctct tgtaacatca
     1561 tgtgttccaa ttaaatttaa attagaccta aatgattctt ttggataatt ttctttaatc
     1621 tgcatatatc tattacaaag ttcatatgaa ttaatttctc cttttaagaa agaaaacatg
     1681 ttatttctaa aaggataacc cataacagaa tctaattctt cacctaataa ataacttctt
     1741 ctctgaccat agctaatctt atttgatgca tcttcccata cttcacctat aagaattgag
     1801 tcatcatctg ccttttttaa ttcctttttt aactctctta taaacttagt tggtaattcg
     1861 tctgcaacat ctagtctcca gcccttaata cccatattta cccatttatt tataacgcta
     1921 tcattgtcat atattatata atccatatag ctatgttcta attcatttat atttggcaat
     1981 gtcttaacat cccaccaact tttatatttt tgaggaaatt cttcaaacat ataccatgaa
     2041 taatatggag attctttaga ttgatatgct cctaaactat tatagtttcc atacatatta
     2101 aaatatttac tatctgctcc agtatgacta aaaactccat ctaatactat tgatatacct
     2161 ttttcttttg ctttatcaat aagttcttta aatgtatctt catctccaaa cattgggtca
     2221 atttttttat agtcacctgt atcatactta tgattactag aggcttcaaa tatagggctt
     2281 agatatagta tacttacacc taattttttt aaatatccaa gtttattgat tatccctctt
     2341 aaatttccac catggaaatc ccatcttata acatcccctt gactatcctt tatgtacata
     2401 ggtgtatctt cccaatttcc atatatgaaa ctattttttt taggattatc aaccttacca
     2461 ctacgatttc cattattgaa tctatctaca aatatatgat aaagtattcc ttctttgtac
     2521 catgttggta ctttaaaatc tttataaaca gtgatttgat atttattaat atcactataa
     2581 ttatattcac aagaatatcc attatctctg ttttttccat aaaataaagt ctttatttga
     2641 ccatccattt caacatcaac tttaaagtaa taaaaataaa cacctgtacc atcaaacttt
     2701 tcaatcttac cacaaaagca ctttccaagt ttatcattat ctaattcttc atttaaagtt
     2761 attgttctca tttcttcttc tctatttatt tctagggaaa tagacttgat attgtatccc
     2821 tcatttactt taacacaaat ggtcaattcc tcatcaaatt ttaaagctcc aaacggagcc
     2881 ttaaaatttt tatcccaaga gttatattct attacacttt gcatttaatc acaccctttt
     2941 aaaccaaatg tcttttgcat actcagaaat tgttctatca gaagaaaaaa atccagagtt
     3001 agctatatta actaaagaca ttttattcca tttttcttta tctctatata acctgtttat
     3061 atctgcttga gcttgaccat aattttcaaa atctctcaat acaaaatact catcattgta
     3121 agtagtgagt gaattataaa tacttcttcc ttcttcgcca agattaggaa taaatccatt
     3181 tattaaatca tctactaccc tttttatatc catattagaa tgatataggt caagtgaaga
     3241 atatccacca tatttattat aatttagtac ttgttctgca ctaagtccaa ataaaaacat
     3301 attttcttta ccaacctgtt cacatatctc tacatttgca ccgtcaagtg tagccaaagt
     3361 tatagcacca ttcatcataa acttcatatt acttgttcca gatgcttcct tagttgtagt
     3421 tgaaatttgc tcactaacat ttgctgctgg tattattatt tctgctaatg atactccata
     3481 attctctaaa aatactactt ttaacttgtc ctttactcta acatcattat ttatagtact
     3541 tgcaacagaa tttataaatt taattataca tttagccaaa taatatcctg gtgctgcttt
     3601 tgcgccaaat ataaaagttc ttgggtcaag attcaaatta ggattatcaa gtaattcatg
     3661 atacatatga agtacattaa atatattcag taactgtctt ttatacgcat gcaatctctt
     3721 aacttgaaca tcaaatatcg aacttgggtc tacatttaaa tcgtatttat cttttataaa
     3781 ctttgctaga tttgctttat tattgtattt tatatcatca agtctttgta aaacagagga
     3841 atcattttta aatttttcaa tattttttaa ttgtaatgta tctttcttcc atgaatcacc
     3901 aattaaatct gtaattaaat tgctaagttg aggatttgaa cttataagcc atcgcctatg
     3961 tgcaattcca ttagtcttat tattaaattt atttggttca tcttgataga aatcttttaa
     4021 tacctctgtt tcaagaatat gagtatgaag cttagcaact ccatttacac tatgacttgt
     4081 gacaatacat aagtttgcca tattgatatt gtcacagtct attatactca ttctttttat
     4141 tctatcttgg tcatatcctt tattatttaa ttcctctaca tatcttctat ttatttcttc
     4201 tattatcata taaattcttg gtaatagctc tttcatcata cttacaggcc atttttccat
     4261 tgcttctgac attatagtat gattagtata ggacactgtt tttttagtta tttcccatgc
     4321 ttcatcccaa gataaatttt cttcatcaag caaaattctc ataagttctg gtatgcataa
     4381 ggtaggatgt gtatcattta tatgaattgc taccttatcg aataaattat taatatttaa
     4441 tttgttcttt ttatattttc ttattatatc ttgtatccct gcacttacaa aaaagtattc
     4501 ttgtttcaac ctcaacaact tacctgcata gtttgaatca tcaggatata atacctgtga
     4561 tatttcctca gtataatact tatgttttaa agcctcttca tagctaccag aataatttag
     4621 agcatttgag gtaagttcac caaaatctct ctttggtatt tcactcttaa acaatcttaa
     4681 agtatttata cattggtttt ggtatccgat tattggtata tcataaggca ttgccattac
     4741 aggaaggtag tttttatgaa ttacttttaa atgactacct tccttgatta attctacctc
     4801 accaccaaac ttcaccattg ttgcttcatt tggtcttaca gtttcccatg catatcgtcc
     4861 ttctgtaagc cagttatcag gcacttctac ctgataacca tttacaaatt tttgttcaaa
     4921 cagaccatat ttatacctta ttccatatcc ttgaccactt atgttcaaag atgccataga
     4981 atctaaaaag caagctgcta atctaccaag tccaccattt cctaaggctg ggtctacttc
     5041 agtttcaatt aaatcatcca agttgattcc taattcagat aaaccttctc taacttcttc
     5101 ttcaatattt aaatttaata aattagattt taactgtctc cctattaaaa attctaaact
     5161 aaaataataa acttcttttt tcttatctaa ctttgtagca acccattttt ttgctatctc
     5221 atctttcaat agactacata atacatttaa caattgttca tcagtagctt ccttgataga
     5281 ttgtgcatat aagctataca tttttacttc aaatttttgc ttgaatttct ttttgcttat
     5341 tgttaccaaa aaaaccactc ctttaatcac atatgtctct atatgtttct atatactcct
     5401 tagctgattt tttccaacta ttatctgttt tcattgcatt ttcaactaat ttaatccatt
     5461 tttctttgtc cttaaatact ttgattgcat ttttcaaaca gaaaaacatc tcatgagcat
     5521 tgtaattttt aaaactaaat ccattgcctt ctccagtaaa tttattataa ggtgttactg
     5581 tatctctaag ccctccagtc tctctaacta ttggtaaaga accatatctc atagctaaca
     5641 tttgacctat accacaaggc tcaaataatg atggcattaa gaacatatca cttgcagcat
     5701 atatctgttg agccaatgat gcattaaaag taattctagc tgatacttta tctgaaaaat
     5761 tagaatcgta atagttgaac attgattgat attgatgttc tccagttcct aaaacgacaa
     5821 tctgtaaatc ttctctcata agttctggca tcatgtatgc tattaaatca aatccttttt
     5881 gagaaactaa tctcgaaact atacctatca acggaataga tgaatctttt ttaagtccca
     5941 atatatcttg taatctcaat ttattttcta cttttttgtc aattgagtct acatcataat
     6001 gaacaaatat atctttatct tttgcagggt catttaaatc ataatctata ccatttaata
     6061 taccttttaa ttttcctgac tcttttctaa gtaatccatc taattgttca ccataaaaag
     6121 atgtttgtat ttcatttgca taagtaggac taacagttat aattttatct gcaaaattta
     6181 taccagcttt cataaaattt attgagccat aatactctat atctccatta tcaaagtatt
     6241 tttgtggtaa gtccaatata tcatataaaa catgcttatc aaatacacct tgatattgga
     6301 gattatgtat agtatacata gtttttatat ttttataccc ctcaagggtt gcatatcttt
     6361 cttttagaat taatggtaac atccctgtat gccaatcatt tatatgtatt acatctggat
     6421 aaaaatcaat tctacttata gcttctaaaa ctgcatctgt aaagaaagta tatctttctg
     6481 catcatctcc atatccatag atactctttc tttcatcttc tcttctaaaa taaaattcat
     6541 tatctataaa gtaaaatttt acaccatcta attccatttc taaaagacca cagtattgat
     6601 ttctccatcc aacacgtaca ctaaacacag ctatttcttt tagttgatct tttaaataac
     6661 ttggtatagt tgaatatttg ggcattatta cccttacatc tacaccttct ttttttaatt
     6721 ctttaggcaa tgcataagaa acatctccta atcctccagt ttttgcaaag ggccaacact
     6781 ctgctgttac ataaaaaacc ttcattagtc attccccctt ctaaaaaatt tatttatata
     6841 tttgtattct tttccactac aagaggatat ttttcatctc ctcttaattc tttcccattt
     6901 gatacactcg tatttttatc taatattgca ttatatagtt ttgcatttga ctttattaca
     6961 caattttgca ttactattga gttctctata actgttcctt tttcaacatt tacctttcta
     7021 aatatgatac tatcttttac agtaccttct attaaacaac cagatgctat aaagctattt
     7081 tctactttag cactatctgt ataaattgta ggtgattcat tcttttcttt tgtaaatatc
     7141 tttctttcag aatataataa ttcatctgca acttctaaat ttaataaatc tttgctcatt
     7201 tcaaaatatg attcaacaga atttacacat tttagatatc ctgtatattc ataggctcct
     7261 attttcatat tttctgcttc ttcaataata aattcttcta aataacaata attgccttta
     7321 tttacaatgt tatatataca ttcaacaaaa tcttcacgct tcataatata tgtctccata
     7381 catatgtttg ctctagggaa cttacctaaa ttatttccta catttattac cctattgtta
     7441 ctatctaaat ttagagttaa gttaccagca aaatctttat ttgcattatt tacattttta
     7501 tatattatag ttatatcatt atgagatttt ttatggtatt ccaatgcttg agaataattt
     7561 aaattgcata gcatatagct tggagctatt aaaatatatt cttcttcact cttttctata
     7621 taatctagat tagctaagat agtatatata tcaccttctc tatgtgagta agtatttcta
     7681 tacttagtgt tttctggact aaaaattgat aggccacctt tttttctact taaatcccaa
     7741 tctttaccac tacctatatg gtctgttaat gatctgtatt tttcttttgc gaatattcca
     7801 actttagtga ttcctgaatt tatcatatta gataaagcaa agtctattat tctatatctt
     7861 ccagctattg gtgtagatgc tattggtctt ccatagttaa gtttgtttat tgctgggtct
     7921 ccttttttgt ttaaatttat tatccctaaa cattcatttc tcatcttaaa gacctccttc
     7981 tattaatacg catctaggtt cgatattttc atactcagaa actacattta tttcattatt
     8041 tacattcttt atctgaacat tatcatttat agttgcacac tcacctatca tagctttttc
     8101 tatagttaca ttttcaccta ttacaacatt tggcattatt actgaatcaa ttactttact
     8161 tcctttacca acaacaactc catgtgataa tacagagtta cctacttcac ctaaaattct
     8221 acaaccatca gctatcattg atctatgaac atttgcattt ttacctatat attgtggagg
     8281 cattgccata gtatttgtat atatcttcca cttagggtct gctaaatcaa gtgtttctgg
     8341 agattttatt atatccatat tagcatccca tagactttgt atagttccta catctctcca
     8401 gtatccttta aatggataag catacattcc aacattatct tctaacattt taggtattaa
     8461 attttttcca aaatcatcat aatttatttc tggatttttt tctgcttcct taaaataatt
     8521 tctcaacatt ttccaatcaa atatgtatac tcccatagat gccaaattgc tcttaggttc
     8581 acttggtttt tcttcaaatt catagatact tgaatcttca tttgtattca ttatcccaaa
     8641 tcttgaagct tcatcccaag gaacttcaat cactgctatt gtagctttag agcctttttc
     8701 cttatgaaat tttaacattt tgctataatc catcttgtat atatggtcac cagataatat
     8761 caatacatat tctggattat aggtatctac aaagtccata ttttgatata tcgcatgtgc
     8821 tgtaccatta taccaatttc cttctttttc attcataaat ggttgtaaaa catagacacc
     8881 accatttatt ctgtccaaat cccaatgact tcccatgcct atatgtgaat ttaatatcaa
     8941 cggtctatat tgagttaaaa cacctacagt atcaatacca gagtttgaac aattgcttaa
     9001 cacaaaatct ataattctat acttacctcc aaatgataca gctggtttgg caattctttt
     9061 tgtaaaaaca cctaatcttg aaccttgccc tcctgccaaa atcatagcta acatctcttt
     9121 tttcatttaa tgacactccc ctcatctagt aattaatatt tataattgca tttttacttt
     9181 tataaacttt atcagtcaat aaatgtcgtg tataaatgtt gcttaaataa taccttaata
     9241 aaaataaaat aatatcaact atacaattga tataatgtat accccataat ggacttaccg
     9301 acgtatacat tattttccat aagcactata gtatttatta atattcaatt attggttaaa
     9361 aaataataca aattttattt tataaatata aaaacatttt ttataaatcc tttgtgtcat
     9421 atgaacatgc ttatttttta atttgttaaa ttcgtaatag caaaatataa tctaatattt
     9481 gttaaataat tatccaaag
//
LOCUS       DAMKMI010000145         9451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMKMI010000145 DAMKMI010000000
VERSION     DAMKMI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.151"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            491..1696
                     /locus_tag="PC429_003157"
     CDS             491..1696
                     /locus_tag="PC429_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF3848638.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1767..1988)
                     /locus_tag="PC429_003158"
     CDS             complement(1767..1988)
                     /locus_tag="PC429_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848639.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2129..2728)
                     /gene="recR"
                     /locus_tag="PC429_003159"
     CDS             complement(2129..2728)
                     /gene="recR"
                     /locus_tag="PC429_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF3848640.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2741..3091)
                     /locus_tag="PC429_003160"
     CDS             complement(2741..3091)
                     /locus_tag="PC429_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF3848641.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3150..4787)
                     /gene="dnaX"
                     /locus_tag="PC429_003161"
     CDS             complement(3150..4787)
                     /gene="dnaX"
                     /locus_tag="PC429_003161"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF3848642.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4864..5128)
                     /gene="ffs"
                     /locus_tag="PC429_003162"
     ncRNA           complement(4864..5128)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC429_003162"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5166..5257)
                     /locus_tag="PC429_003163"
     tRNA            complement(5166..5257)
                     /locus_tag="PC429_003163"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5221..5223),aa:Ser,seq:gga)
     gene            complement(5258..5713)
                     /locus_tag="PC429_003164"
     CDS             complement(5258..5713)
                     /locus_tag="PC429_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF3848643.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(6034..7305)
                     /gene="serS"
                     /locus_tag="PC429_003165"
     CDS             complement(6034..7305)
                     /gene="serS"
                     /locus_tag="PC429_003165"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF3848644.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7431..7636)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7764..8645)
                     /locus_tag="PC429_003166"
     CDS             complement(7764..8645)
                     /locus_tag="PC429_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF3848645.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8657..9166)
                     /locus_tag="PC429_003167"
     CDS             complement(8657..9166)
                     /locus_tag="PC429_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF3848646.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(9292..9408)
                     /gene="rrf"
                     /locus_tag="PC429_003168"
     rRNA            complement(9292..9408)
                     /gene="rrf"
                     /locus_tag="PC429_003168"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3007 a         1655 c         1013 g         3776 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taattttttt
      181 atttatcatg tattctttat ctttaatttt taaaacttaa tgctaagtaa acctgtttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatataaa tactatgcta cattttagga
      481 ggattttatt atgagcaaaa aagtattaat aatgtctgct tccactggtg gtgggcataa
      541 tagggctgct ctagctatta aagaagaact tacttccaaa acacttgatg gagagccaat
      601 tgaatgtgaa ataatagata gtttgaaatt agttaataat acaatggata aaataatttc
      661 aagaggatac gaaaaatctg ctatatatac tcccaaagcc tatggaagtg tatatagatt
      721 atctgaaaca aatcttttgt caaaaaatga atttaaagat aatctactaa taacattcat
      781 ggctaaaaaa tttaaaaagc ttattagaag cgaaaaacca gacttgatta taggaacgca
      841 tccttttcca atgattgctc taagtacatt gaaaaaaaac tttaatttac ataataatga
      901 atcaaatgct tacacagagc atttttataa acattataca aataccatta atgttcctcc
      961 gcttatttct gtattaaccg attatactac tcattctaca tggatacaaa atgaaattga
     1021 ttactatatt gttggtcatg aatatgtaaa agaattattg gtgtttgatg gagttgaacc
     1081 gagtaaaatt agaacatttg gaatccctgt tgaaaaatct tttctctctc acagagataa
     1141 agatattgtt ctttctgagt tgaatttatc tcctgataaa ttaactgttt tacttatggg
     1201 gggtagcttt ggggctggaa atataaaaga gacacttgat gaattactgg atactgatag
     1261 agactttcaa atacttgtaa ttactggtaa gaatgaaagc ctaaaagaaa aaatcgaaaa
     1321 aaaacttatg tctcgttatc atgataaaaa tgtatgtgtc cttggataca ctgataagat
     1381 gaatgatatt ttagcttcta tagatgtatt agtaagcaaa cctggtggac ttactacaac
     1441 tgaggctctt ttaaaagatg tacctatgat agttccttat tatataccag gtcaggaaga
     1501 agagaatctt gatttcttat ctaattgtgg tgcagcactt agaactacaa aaaaatataa
     1561 cttgtcaata ttactaaaag ttttaataga tgaccctagc agattagaaa tgctaaaaag
     1621 aaatattaaa tcaataagaa agtcaaattc agcccaaaat atagctaatt taattttaaa
     1681 tataatttct ggttgataaa ataaataaaa tccctatagt atatatatta tctattaggg
     1741 attttttatt ttctaattat ttaatattaa attggctcat aaaagtcttc ttcataatca
     1801 tactgataat ttttataatt atttctaaat tcagcatttt tatttcgtac atttttttta
     1861 gtttttttct tattagaaaa acttatatcc tcaaaatcat catatttagg tttatctttt
     1921 gatttcattt ttttaactct tttttctttc attgcattat gttcttctaa gttcataatt
     1981 ggtttcatgt tgcaccccta tcaaaatttt taaatgattt atacaacaaa ttatacaata
     2041 cagaagaaat ttattcaata aatctaaatc aaaattaatt tgaatttaac tcaaaaaact
     2101 acaaaaatcg aggtattaac ctcgattttt atatttctct tctgccttct agtgcttttg
     2161 atattgtgac ctcatctgca tactctaaat cgcctccaac aggaagacca tgagctatcc
     2221 gagtgacttt gattcccatt ggttttacta acctagcaat atacatggct gtagcttcac
     2281 cttcaatagt aggatttgtt gccattatta tttctttgac ttcctgattg ccaagtcttt
     2341 ggataagttc ctttattttt aacatatctg gtccaattcc atccattgga gaaattactc
     2401 catttaatac atgatactgt cctttaaact ctctcgttct ctccatagct gctacatctc
     2461 ttggatcttc cacaacacat ataacacttg aatctctact tttatttgaa cacatgctac
     2521 atgggtctgt atctgttata ttacaacaaa ttgagcaata tcttatttct ctttttgcat
     2581 ctataatcgc tttagataaa gcttccacat cattagtatt catattaata atatgaaaag
     2641 ccaatctttg agcagtcttt cttcctactc caggaagctt tgaaaattcc tctataagtc
     2701 tcgttatggg acctgtataa acttgcataa ataccaccta ctagaataac ccaggtatat
     2761 tcatacctcc agttacctta ctcatttgac ttgcttgtat atcatcaata tttctcaatg
     2821 cttgatttac tgcacttaaa actaaatctt gtagcatttc tatatcatct ggatcaacta
     2881 cttctggctt gatagttata tctataactt cttttttacc attaactttt acagtaactg
     2941 ctcctccacc aactgaagct tctacttctt tactttctaa ctcttgttga gctttttgca
     3001 tattttcttg cattttttga gcctgcttta acaagttgtt catatttcca ccaccaggca
     3061 tcattccgcc tccaaaacct tttttagcca taaacactat tcctcctaaa cttattttca
     3121 ttttattatt atatcataat agtattttat tatttactct cattttcatc aatactatct
     3181 tttatttcta atatctcttc actcactatg ttctttaata tttcctcacc aatgtccttt
     3241 ttctctttaa cttcaagatt tatatcttta acttctgatg tcagtactat ttttacatta
     3301 aaacttctat ttaaaacatc tcttattgtt ttttctaaat aatttattgt atcttgagag
     3361 ctcagtctag tttttgcaaa atcaaattta tcatcaaata taacatacaa catattagaa
     3421 tatacattaa aactttttac ttcacttaag agtgcgtata ttggcatttt tttatctttt
     3481 ttaatttgtt taagtatatt cttccaagat gactctataa gtataacatc ctcatttttt
     3541 acatcttcat aaactacatt ttcttgtttt acatctattt ctctatcgac ctcatactct
     3601 ttttctttat tattcccaat tttttcagac ttaaagttcc ccagttcaat cattttttct
     3661 aagttctcaa ctctttttat aagagcttct ttactttcat caaacattgg ttgagcaatt
     3721 ttcattatgg ttatttccac taaaaccctt ggatttgaag atgactttat atcatcctga
     3781 gttattgata atatatttaa aatccttatt aaatcattta tatctatatt tttagactga
     3841 attttaagtt gttctatagt ttcttctggt aatgaaatta tttcatccaa ttctgatgaa
     3901 accttacata ccattaaatt tctaaaatga tcaattaagt catttataag atttcttata
     3961 tctttgcccc ataagacaaa ttcatttaat atctcaagag actttttagt gtcctgctct
     4021 acaatacatt gagacaattc aaataattgt tctatattta cgcttcccat taattcaaca
     4081 acatctttat aatctatctt actttctcca aaagacatac attggtctaa tatactaagt
     4141 gcatctctaa gagctccttg agaatttcta gctattaaat taagagcttt atcatctata
     4201 tcaattcctt catcttcaca aatctttttc attctaaggg tcatatcttt aactgtaact
     4261 cttttaaaat cgtatctttg gcatcttgat aaaatggttg caggtatttt gtgaggctct
     4321 gtagttgcta atataaaaat tacatatgat ggaggttcct ctaaggtctt taataatgca
     4381 ttaaaagctc cttgagacaa catatgaact tcatctatta tatatacttt atatttagca
     4441 ttggctggag aatatttcac actttctcta agctctctta tatcatctac actattattt
     4501 gaagctgcat ctatttctac aacatccatt gtattatctt ttaatacaga ttcgcatatc
     4561 tcacactcat tacacggttc ttcatttatt ttattcaaac agtttactgc tcttgaaaaa
     4621 atctttgcag tagatgtttt accagtacct cttgttccac aaaacaaata agcatgtcct
     4681 atattatcac tatatatttg attctttaaa gtcttaataa tatgatcttg accaataaca
     4741 tcttcaaatt tttgtggtct atatgctcta tataatgctt tatgcatctt tttacctctc
     4801 tttagcaaaa ttaataaaaa aatccctcat tatctaaggg attagtcttt tatatattaa
     4861 atgctgtaca ccatctatcg acttaattcc ataggcgtta ccaaaacagt tagctcaact
     4921 taggcacccc cacggcacac aaaagtgatc acttaccgct gcttccttcc ggatctgacg
     4981 gggttcatga gctttcattg cgcaggacct aagcatcaac accacttatt tagggcggcc
     5041 ctacagaaaa taagccttta gatggaattc aatcctgcta tagcggattg caggttacag
     5101 ggcaccgcta cctccccatc tagtacagca aaattacaac catatttaat tactttcatt
     5161 gtttctggcg gagagagtgg gatttgaacc cacgatacag ttttacccat atactcgctt
     5221 tccaggcgag cgccttcgac cactcgacca tctctcctta atctcttttt ctcaatgcct
     5281 taaaaaattc ttgcaatatg atcgagcatt cttcttgcaa aacatcaaaa gctactttta
     5341 cattattatt attaaaataa tctaatttaa actcatgctg tttttctata tatgaatttt
     5401 ttacatgtct tgtccctatt ataagctttt ttattctaga attaactatt gcgccactac
     5461 acattatgca aggttccatt gtaacatata aatcacaatc agtcaacctc caaccaccta
     5521 atttttctga tgcttgtttt atcgctaaaa tttctgcatg tgccgtagag tcttttaacg
     5581 tttctgtaag gttatgagct ctagctatta tttgattatc tttaactata atagcaccta
     5641 ttggtgtttc ttttttatta tatgctttat atgcttcttt taatgcttct ttcatataaa
     5701 atgaactttc cataattcct cccaaaactt ataacaaact tatcttatca taattcattt
     5761 tataattcaa cttaatttta ttttgtttat ttaattatta ctatatatag ctatattttt
     5821 tttaaattag ttctaaaata atttttagat attcatacaa gagtgtttta taaaaatatt
     5881 tagtgtttta tgatgaaaat tctgctgtgt aaattaacat aaaaaaacta tgaaattaat
     5941 atttataata atttcatagt ttttttattt attgtttact cataatatat tctcttcatc
     6001 aaagcatact ctacatatac aattaatata aatttatttt ataacatcaa cacccatata
     6061 aggtcttaat tcttctggaa ctactacaga accatctgcc tgctgataat tttctaatat
     6121 agcagccaaa catcttccta tcgctaatcc agaaccattt aatgtatgaa catattctgc
     6181 ttttgatttt ttatcccttt taaatcttat tcctgctctt cttgcttgga aatcttcaaa
     6241 atttgaacaa gaagatattt ctacatatct attgtagctt ggcatccaaa cttctaaatc
     6301 atatttaaat gcagctgtaa atcccaaatc tcctgtacat atttttacaa ctctatatgg
     6361 aagacctaac atttgtaaca tagtttcagc atcatgagtt aatgattcta attcattata
     6421 agaatcttct ggtttaacaa atttaactaa ttcaacttta ttaaattgat gttgtctaac
     6481 taaacctctt gtatctcttc ctgctgaacc tgcttcagat ctaaaacaag gagtatatgc
     6541 acagtattta attggaagct ctgcaacatc taatatttca ttagcatgta tattagtaac
     6601 aggaacttca gctgttggta ttaagaaata ctctaatcct tctattttaa acatatcttc
     6661 ttcaaattta ggaagttgac cagtccctat aaaactattt ctattggcca taaatggagg
     6721 taagacttcc gtatagccat gcttagctgt atgagtattt aaaaagaaat tcattaaaga
     6781 tctttctaat ctagctccta atcccctata taaagtaaac cttgacccag ttattttccc
     6841 tgctctttca aaatctaaaa tacctagacc tgtacctata tcccaatgcg ctttatgctc
     6901 aaaatcaaat ttagtaggtt ctccccaagt tcttatttga acattgtctt catctgtttc
     6961 accttgtggt acctctggat gaggaacatt tggtattctc attaatgtat attccatttt
     7021 tgcttctact tcttttactt tctcatcaat accttttatc ttatctgata actctttcaa
     7081 tcttgccttt tcttctgtta catcttttcc ttcttttatt aattgaggta tttttttaga
     7141 atttacattt aactcatttt tcataacttc aacttcttga agtatttctc ttcttttatt
     7201 atctaattcc actacagcat caaggtcaaa ttctctttca cctcttcttt ccatagcttt
     7261 tttgatatca tctagatttt ctcttattct tttaatatct aacatattaa ttcctcctaa
     7321 taattttgat aatataagtt ataattccaa cttaatttac aaacattata cttgtaaact
     7381 cttagtttaa acaaatttta aaaataaaaa aagacctccg tttaccctat aaaagggacg
     7441 aaaatctttt atccgcgttg ccaccctaat tgactatact aaaatgtata atcctctcag
     7501 acctataacg taggtaaacg tctagcttta actagaagct ctagagtaga ttcaaaaacc
     7561 attgtaatga gttttcacca tccactcatt ctctgaatac aatctgtttt ttactatttt
     7621 ccttcacagc tttttaacta attgttttat ttgataatca ttatattata tatattaaca
     7681 tctttcaata ctattattca aattttttta gaattttctt taatttttta tattaaattc
     7741 attttttcta tttaattaag accttatctt ttatccattg tatttagatt acctgtatta
     7801 ttaaataata atgaagaagg aacaaatgga acttttatat cattctcatc aaatttattt
     7861 tttacctttt ccattacatc aaagtataca tcccagtaat catcagtttt tacccaaggt
     7921 ctagcaacta tttgcatggt gttatttcct atagagttta gacctattat aggctttgga
     7981 tcatttaata ttcttttttc gtttgtaaaa atttcattta atatagactt aactacatct
     8041 atatcactat catatgaaat attatatgaa aaatctattc ttcttttgtt ttgatgtgtg
     8101 taattaatta tattattact tgtcagttga aaattaggta taattatagt tttattatct
     8161 ggcgtcttaa gaacagtgct aaatatttgt atgtcagaaa ctgtaccttc tacacctgaa
     8221 ccttgaatat aatcacctat attaaatggc ttaaaaaata aaataatcat accagagcct
     8281 aaatttgata atatttcttt aaaagctaaa cctatactaa atccagcagc tcctaacaca
     8341 gctacaactg atgtagtttg tatccctata atacctaagc ttatcaatga tataatagat
     8401 aacatagata tatatattcc atatacaata aaattattta cacctttact cattccatat
     8461 ttgattagaa actttgaaac catccttctt actattttag atatataaat acctatcaca
     8521 aatacaatta ttgcatatat aatatttggt atattatcac taaaattatc gagcatattt
     8581 tctaaagttc tcttagttat attgctattt gttgaggcag tcttagccaa tgataaaaaa
     8641 ttcattcaac tcctccttat gataatattc cgttgtcctt taactctttt actatagctt
     8701 ctatagtttc ttcttttatt gcctcaattg tatgaatatg gctacattct gtcagaaccg
     8761 ataattgttt aaattcattt gtctcaactt tcttcataaa gttgtctata tccattttag
     8821 aataaatatt aagttctgca gttatctgac catatgttgg atgatttaca ataacatctt
     8881 ttataatacc acctaaatct acaattatct ttaattcatc ataaatttct ttagaatcct
     8941 tatgattttt acactttatt tgttttgtaa aatacggttg attatatatt atatatcctt
     9001 gaggtgtagc tattatttca aatcctctag ctcttataag ggctatatct tgaactatta
     9061 cttgcctact tacatttaat agttcagcta gtgttccacc ttttacagga tgcttagatt
     9121 cttttaaaat atctattaat ttacttcttc tttcattaga attcatatta caccttcttt
     9181 ccaaataaaa actttaccta tactatataa ttttatattt tatcttatta taaatcaata
     9241 ttgactttat attctattta atcttaactt ttataataaa taaaaaagac tacgtggcta
     9301 cgtcctactc tcccaggcag tttcccacca agtaccatca gcgctagaga gcttaacttc
     9361 tgtgttcgga atgggaacag gtgtatcctc tctgctattg taaccacata atctttatgc
     9421 ttaatatttc tattaagttt ttaataagtt t
//
LOCUS       DAMKMI010000146         9420 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMKMI010000146 DAMKMI010000000
VERSION     DAMKMI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.152"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(18..707)
                     /locus_tag="PC429_003169"
     CDS             complement(18..707)
                     /locus_tag="PC429_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF3848647.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            complement(700..1071)
                     /locus_tag="PC429_003170"
     CDS             complement(700..1071)
                     /locus_tag="PC429_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF3848648.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            complement(1253..2599)
                     /locus_tag="PC429_003171"
     CDS             complement(1253..2599)
                     /locus_tag="PC429_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF3848649.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMFYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            complement(2781..3374)
                     /locus_tag="PC429_003172"
     CDS             complement(2781..3374)
                     /locus_tag="PC429_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF3848650.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            complement(3439..4908)
                     /locus_tag="PC429_003173"
     CDS             complement(3439..4908)
                     /locus_tag="PC429_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF3848651.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            complement(5023..6414)
                     /locus_tag="PC429_003174"
     CDS             complement(5023..6414)
                     /locus_tag="PC429_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF3848652.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            complement(6580..6828)
                     /locus_tag="PC429_003175"
     CDS             complement(6580..6828)
                     /locus_tag="PC429_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848653.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            7094..9223
                     /locus_tag="PC429_003176"
     CDS             7094..9223
                     /locus_tag="PC429_003176"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF3848654.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
BASE COUNT         3098 a         1502 c         1042 g         3778 t
ORIGIN      
        1 catcttaatt ttatttttta tataaaattc attataattg gaattagtat tactgtaatt
       61 gtaccagaga ctccaataga aacaccactc atagctcctt ctatttctcc aagttcaaaa
      121 gcctttgtag ttcctaaagc atgtgcagat gttccaagag caattccttt agcaactgga
      181 tgatttatat tccccagttt gaatactgta gaagcaacca taccaccaaa aatacctgta
      241 aaaattatgg ctacaactgt tattgattct acaccattta aagaatttgc taaagatatt
      301 cccattggtg ttgttataga ttttggaatt agtgaattta ttatacttac atctgaatta
      361 gctattttgc caattatcaa tacagataca aaagaaatca ctataccaca acctattcct
      421 acaataatct caaacatatg ctttttaaat aattcaaatt gtttataaag tggaactgca
      481 agtactactg tgacaggacc taaaaagaaa tttagactat cagctcctat cttgtaattc
      541 tcataaggga ttttagatat gcttaaaaac aatattattc ctacgattgc aattaataaa
      601 gggttacata taggattact tgtttttcgt tgaatgtatc ttcctaaatt gaaaaaaact
      661 attgttacta ttataccaaa tactggtgtc tgtaatacat tatacatttt tttgtccttc
      721 ttccttcttt tttatcattt tctccacaat aattccagta gtatacatta ctattagtgt
      781 acttaaacat atgactaata aaagcactaa tacattttcc cttattaggt ctaatgactt
      841 tataagtgat acacctgctg gaataaaaaa tatagccata ttatcgagga aaaatccact
      901 tacattttct atactactca agtctaaaac tttaaattgt agtagtagaa ataataatag
      961 cataccaact atactcccag gaataagtat aattccttgt ataaaggaac ttatatactc
     1021 tcctattacc cagatagata aaattattac caattgattg aaaactttca ttttccacct
     1081 ccaaaaatta aagagattgc ttcaaatcat atatttgtag ttttatcaac aagcaaatat
     1141 ctttctttaa aatagaaagt tataatttga cacaatctct ttttaaattt atttttattt
     1201 taatttaata aaatacatac ttggttgttt caaaaaactt aactatataa acctattttt
     1261 cacttaaaat ttcttttatt tttgaattgt tatacatact gtctggaact tcacttctca
     1321 aactataaaa agtctgttgt gcatttttta aatcgccatt atcataataa aacataccca
     1381 actcgtaata tgaatcatca taatactggt catttttagt ataagtattt atatattttt
     1441 tgtaatattt tatagcttca tccttatttc ctaataattt atttgttatt gctaattgat
     1501 atattgactc agaaatatat tttttagttt taccagaaga aactacactt ttaaaattat
     1561 ctatggcttt ttcatattct ttgtttttct ttaaatttag agcttgataa tataaatctt
     1621 cctcatcagc tacagttatc tctctttctg gttctttatt tttaacctca tttaattcct
     1681 tattcgtttt ttctaatttt ttactcgttt ctcctaactt tttatcagtt tcacttaact
     1741 tttcctcttt actacttatt tggttattca agttttctat tctctttttg ctttgaatca
     1801 aatatatccc catacctatt aaaacacagc caactataat atatggcaaa atacttttct
     1861 ttctttttac tttttttatt tcttcctttt tacttgtatc ttctttttct tcttcatttt
     1921 tttcaaatac aggattatct ttatctagct ctttaagttt gtctaaatat ccttctctct
     1981 tttttacatt acctagttta tcatataaca aagataaaat tacatatggc tctattagtt
     2041 caggttcttc ttcaattatg ttctctaaaa tatgtattga ctcataattt aattcttcat
     2101 ttataaatct tatagaatga ttgtatcttc ctaaaaaagt tttaaaatct tcagatgaaa
     2161 ttagatttac atattttcta gatatatgat tattttcata acataaagca gtataaaaac
     2221 tctcaaatga tttagcaaaa tcacatttta ataattttaa aagacctcta agatttagta
     2281 tatcaatgtc tttaggattt atctccagag ctttattaac catattaatg gcactagtta
     2341 tataattttt atatgctaaa tccaatgcat tgttatatct tttatatgat tctaatttta
     2401 actccttaga cgttgtatct gactttgaat ttaaagctcc ttgcaagttt aatagagttt
     2461 cttctttata atttgtagtc acataactct gttttaatgc ttttaatgtt tcatcaccgt
     2521 cttcttcttc tatattaaaa aaagtcaaat cacttttatt atcttctatc aaagatatat
     2581 ctaaaatttt tctaggcatg ctttctatgt cttgttttat atctttaact aaaataatac
     2641 tcacctccac tctaaaatat atatttaaga taacatattt aatatgtcaa gtcaaaacta
     2701 tacatatttt tccaccattc tcataataaa aaaggtaatc ctcttctttt cataaaaagg
     2761 ttacctcttt ttcaatacta ttaactttta atgttgaatt tttttacttt tctatagagt
     2821 gtagctatac ctatatttaa tgcctcagct acttttttca taccttctgt attacttcca
     2881 tatttcttta tggcattttc tatagcttcc ctctcaaggt catctatagt cttaacttct
     2941 tgtttataaa gttcatctag tttaaaagat attaaatcag acatactcct tacaaaagca
     3001 gttaactcct tatcttttga aagtatcttt tctttttgtt cttcattaaa cgcagctata
     3061 cccaagattc caagtatatg ctctcccatt ttaacgggag tacatatatc agctaactct
     3121 ttacaatttt ctctatttgc acatttatta cagttctctt ctatttcttt atttataaca
     3181 taggatttcc catcttttaa aacaagagag tacactgact tctctgatat tgtctctcct
     3241 attttacctc tgaatattcc tgtacctgct atcctagtta aatcttttgt aactaaagta
     3301 acatctacat ccaagacatt agaaatattc tcgcaaatac tttgaacgta attcgttata
     3361 ttatttaaat tcaaagtaat ccctcctatt tagatttata ttttttatta ctctacataa
     3421 atcctattct tgagaacttt aagcttgcgg ttgtggttgt gtctcattag gcttattatc
     3481 agttggtggt gtttctggtt tttgttgatt atttgggttt tgattgtttg tattttgatt
     3541 attcttattt tcgtctgtat ttggtttttc ctctggtttt ggttccatag tcccaactaa
     3601 tataatacct tccttcttag gataatatga attagctatt ttttcagttt taattttttt
     3661 accatttgca tcattaaatg ttctatatgt tgatacagaa tatgcatttc ttccactttt
     3721 gtctactttt tctttacctt taggcattgt agggtcatta actcttttca ttgtagtttg
     3781 tgaaacgcca tctaattttg ttgatattgt tatattttgt ttatcttctg cacttccata
     3841 tatattgcaa acaatttgat ttcctgaaac gtaatttttt atgtaaactg gatgctttag
     3901 attattttta aataagaaat ctattccact atctgcaaca gttgcatctc ttccttttgg
     3961 aacataactt gatggtatag tatgattttt tacattaaca atttctaaac ctgcgtatag
     4021 cacagaattg tataacgtag atgaaacttg acaaactcct ccaccataat cttcttccat
     4081 ctcaccttcc attattacag gtgcactttt atatccattt tccttacttc ttttccctgt
     4141 atgtttatta aatgagaaag tttctcctgg cataagtaat acattactta tattatctgt
     4201 tgctaatttt atgtttatac ttctatttat ttgagaagtt ttaaatgttg ttgaatacgt
     4261 acctaaattt gtatctatat tagcaagttg ctcttttttt acttttgctg gagtagcttt
     4321 tactactaac tcatttttat agttaccttt tttagtttga ttatcaaaat tctccatact
     4381 cttagaaata tccattttaa gaccatcaga atctggtata accttgactt tttcaccact
     4441 tatatcaagt gtagcatctt ttacatctac atctatttct ttagctatct tttccatttc
     4501 tgcttttaat ttatttttat cataatttat cgcaactact aagttgcttt ttttgccaaa
     4561 atttgctgaa atagtttttg atagattgcc aaagaaagca ctctttctat ttaaattata
     4621 tgcgttttct actgttttat ccaggtcagt agataaattt aaatctttgc tctttacttt
     4681 ccagctttta tcattgtaat taaaatcaac attctctaat ttgtattttt tagctaattc
     4741 ctgttttgct tgtgatttag ttaattttcc aatatttact ccattaacat atgtgttttt
     4801 tgctattttc tcccctttaa attgcatgca cattatgcct ataaaaatta ttactactaa
     4861 tgctacagct ccaatagcta taactagctt tttgttcaca ttactcatcc catacttctc
     4921 cttacaataa aattatttat atatccctat tattatatta ctacataatg cgccttaaaa
     4981 ccccaaaaac tttttaatta tatattaatt taatattatt aattaatatt catttagtcc
     5041 attttctgat atggcatata ttttttcatt gtaaatatta atagcataaa cttctttgcc
     5101 tctaaataag cctttattta ctacagcata ataataaata tttttatatt ttccaaacaa
     5161 ttcttctaaa ctctttttac tttttacata tctatctgct ttctctatat ctaatatctc
     5221 aatcttttta tacttatttt ctttttcatc aagagtatta gttaacacaa acttagcttt
     5281 atcttttgta ataccaaatt gataacttcc atcttctaat attctaaagt atccatcacc
     5341 ataatctact ttgtaatctt ttttaccatc tttatatgaa tataaatctt tagtttttgc
     5401 acttaccaaa acttcattta ttaatttatc atactcatat acttcagcat aatagtattt
     5461 attatttttt atcatttgtg gaagtgaatt tatagataga tttgccttgt ctttgccact
     5521 ttttttagct attacattta taatgtcatc agatgttaaa gtatacaatg ataaattctt
     5581 tttatatttt ttttctaaac tctgtacctt ttcatcatct gaataatcgt cttttaaata
     5641 ctcaatatat tgtaatgctc tttcataatc tccagaagta tttgcctctt cagctctact
     5701 tatatagtaa tcatacattt cttcaataca ttgttctttc ccattttgag ccatactgta
     5761 atacttttca tcttctttta atactttatt atacttttca atagcttcat aatacttata
     5821 tgctttttgg tcttttactg ctgagtcata aacatctcta gactctttga ttgtttctat
     5881 attctgtttg tatacatcta ggttactacc cataccattt aatattgaaa cagtacttat
     5941 gtagttaatg gcaatatcat aagtagtatt ttcttcttta tacatttctc taactctatc
     6001 agcttgagct aataagtcat ccacatctat acttattttg tttaattctt tcacggtatt
     6061 tattaatcca ataaaacttt cttgacttat gtctccatct aaatatttat caccactatt
     6121 taatagcaat ttatttattt ttttagaaac agacttatta aacctttcca tttttgaatc
     6181 agaaaacttt ttttctatac ttgtgtagta atcaacagct atagaatact gtcttttctc
     6241 tataatcttt aacaactcat tagcttcatt tcctacacta aactcgttac tacttaagaa
     6301 aaatactatt atcaaagcaa ttacacctaa tgttgataat gaaatcgcaa ctatagattt
     6361 ccttttttca tcaataaaat ctacagcctt ctcaaataaa tctctaaaat tcattatagt
     6421 tccctctcca tttaataatc tttatatttt atggtaaact aaatttctta ttggttatta
     6481 tatcataatt cttgaaaaaa aataaaaaca caagttaaat cgtgttttta ttttacctat
     6541 caatcattat tttatacata aatatttcct ttacataaat tatgatatct ttctttttat
     6601 atccctagaa tgactctttt gttttttatt attactgaca gttttcgttc ttgtcttgtg
     6661 tatagctttg ctatcaaata tttttctagc attgcataaa acaattccaa ttgttaaaca
     6721 aactgtaagc acacttccca tcaaaaagta ataatccaat cctatatgta ctcccataac
     6781 tgaatttatt aacactaatg caaaagtcat tgcaaaacat atcatcatgg agataactaa
     6841 aattcttaga atttttttca atccactcac ctcgtataat cactaaaatt gacactaccc
     6901 cttatccatt actataccaa attagtaaaa aaatagcaat tactattgtc atttatatta
     6961 ggaaaatatt aaatcttttt aataaatatc tttttttgac ataaggatta cttaactttt
     7021 aataaaatag taacactaat atgtattaat taatagaata aaataaatga taatttattg
     7081 aggggtgatt tacatgccaa atttgccaag tttagggtca aaggctcctg attttaaagc
     7141 caatacaaca aatggtccta ttagactctc tgactataag ggtaattgga ttgttttatt
     7201 ttcacatcct ggtgatttta caccagtttg tactacagaa tttttatgtt ttgctaaata
     7261 ttatgacgaa tttaaaaaaa gaaatacaga actaattggt ctaagtgttg atagtaacag
     7321 ttcacattta gcttggatgt ataatatttc tttacttaca ggtgtagaaa ttccatttcc
     7381 tattatagaa gatagagata tgagaattgc caagttatac ggcatgatat caaaaccaat
     7441 gagtgataca tcaactgttc gctctgtatt tattatagat aataatcaaa ttctaagaac
     7501 gattctttat tatccactaa ctacaggaag aaatattcca gaaatactta gaatagtaga
     7561 tgcacttcag actagtgata gagataatat agttactcct gcaaactggt ttcctggaat
     7621 gccagtgatt ttaccttatc ctaaaaacta taaggaatta aaaaatagag ttaacagttg
     7681 taataagaaa tattcatgta tggactggta cttatgtttt gtaccagata attataatga
     7741 tgaagaagtg agcaagaaaa ttgataatac ttgtagctgg aaaaaagaac atactaaaaa
     7801 cattgaaaat gaatgtaact gtgaacatga acatcatgac tacctgaaca aagctcttga
     7861 ctgtaaacaa gaacacaaga ctgatattaa agatgattgc aatcatgaga aaaaacatac
     7921 taaaaatact aacaaagttc acaactccaa acaagataag tttaaagata agtcttgtga
     7981 tgaaatgaat tttaactatg ataaagatga atcttgtgac aaaataaatt ctagctataa
     8041 caaagaagat agtagttatg aagatttcta taaacataat tataaaaact acgattatac
     8101 tagcgaaaaa aatactaaaa aaatagctat gaaaacttta aaagattcta aaaaattagt
     8161 tagaccacaa ataacagacc catacaatcc aatagttgaa aatgcaaact gtccagatat
     8221 aaatccaatt gtagcagaat atgttcttgg aaatccaact aatgtagatg ctcaactatt
     8281 agatgcagtt atatttgctt ttgctgagat agaccagtct ggaaatttgt ttattcctta
     8341 tcctagattt ttaaatcaat tacttgctct taaaggtgaa aaacctagct taaaagtaat
     8401 tgtagctatt ggaggttggg gagctgaagg attctctgat gcagcattaa cacctacatc
     8461 tagatataat tttgcaagac aggtcaatca gatgataaat gaatatgctt tagatggaat
     8521 agatatagac tgggaatatc ctggaagtag tgcatctgga ataacatcaa gacctcaaga
     8581 tagagaaaac tttacactct tactaactgc cataagagat gttataggag atgataaatg
     8641 gcttagtgta gctggaacag gagatagagg atatataaat tcaagtgctg aaatagataa
     8701 aatagctcct ataatagatt attttaatct tatgagttat gattttacag caggtgaaac
     8761 aggcccaaat ggtagaaaac atcaagcaaa tctttttgat tcagacttat ctttgccagg
     8821 atatagtgtt gatgcaatgg taagaaatct tgagaatgct ggaatgcctt ctgaaaaaat
     8881 ccttcttggt ataccatttt atggaagatt aggtgctact ataacaagaa cttatgatga
     8941 gcttagaagg gattatataa ataaaaatgg atatgaatat agatttgata atactgctca
     9001 agttccatat ttagttaagg atggagattt tgcaatgtca tatgatgatg ctttatcaat
     9061 attcttaaaa actcaatatg ttcttagaaa ttgtctaggt ggtgtattct catggacatc
     9121 aacttatgac caagcaaata tactggctag aaccatgtct attggtataa atgaccctga
     9181 agtattaaaa gaagaacttg aaggtattta tgggcaattc taatagaatg taggaaaatt
     9241 ttatagttat ataaaaagca gattttatta tattaaataa gttgtatcta attaatacta
     9301 ttattaattg tttataaatt agatacttat tttttcataa tacaatctgc ttttttaatc
     9361 aaaatttatt taattataag ttgtttaatt acaagttgtt taattacaag ttgtttaatt
//
LOCUS       DAMKMI010000147         9134 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMKMI010000147 DAMKMI010000000
VERSION     DAMKMI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.153"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            98..1297
                     /locus_tag="PC429_003177"
     CDS             98..1297
                     /locus_tag="PC429_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848655.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEDSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1306..2118
                     /locus_tag="PC429_003178"
     CDS             1306..2118
                     /locus_tag="PC429_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848656.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2129..3397
                     /locus_tag="PC429_003179"
     CDS             2129..3397
                     /locus_tag="PC429_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848657.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKIDIKNKSYKLLNRFNLGLQNG
                     EQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGHDSYSNFIIYDIEDNTFNISNKFMDN
                     LNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIVTDMSNSITNLVYDINSNEIKLEDYE
                     ILDLKVDYNEASSGVRKVKLLDDKIYSISGIKNSISGELKGFKGIVDVVGTKPVKFQV
                     FDMKLKKNIYEAELINGDISLQEKLFFVAN"
     gene            3763..4944
                     /locus_tag="PC429_003180"
     CDS             3763..4944
                     /locus_tag="PC429_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3848658.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(5086..6003)
                     /locus_tag="PC429_003181"
     CDS             complement(5086..6003)
                     /locus_tag="PC429_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848659.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILAAIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            6256..7050
                     /locus_tag="PC429_003182"
     CDS             6256..7050
                     /locus_tag="PC429_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848660.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            7051..9111
                     /locus_tag="PC429_003183"
     CDS             7051..9111
                     /locus_tag="PC429_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3848661.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         3649 a          902 c         1450 g         3133 t
ORIGIN      
        1 tagcagggtt attaagtttt ttaattataa tgtttattct aaatggaata tcttatttgc
       61 taattaaaaa gaaagtatct ctttaaggag gggtggtatg aaattaaaat ctaaaatatc
      121 tatacttatt atatttttga ctttagcaat atattttagt ataggtttat taaataatga
      181 agataattta aagataaaaa ctataaaagg tgactctaat gaattagacc atataaacat
      241 aaattttgga tatgctttga atggatatat aaattcatat tatacaataa atggaagcaa
      301 caaaactaaa aagactgaat taataaaact acaaaaggat agtaagaaca caaataattt
      361 aattgatgta tctagtggtt cagatttaat tgctcatgtt ggatttgaaa tgaatgagga
      421 gtcaaaagat ataaactttg tgatagatga gaaaaaagaa gatttagaaa aaggtgtatt
      481 tccatcagaa gatagtgata aagaaattga atatgaaaaa ttagatatga ataattttac
      541 aaggacaaga tataaagtag ataaaagtac agatgattat tatgcaaaga tactatcatc
      601 aaaaagatat aaaaaatact tatatgtatc tgtactttat gctacaagtg attcatcaga
      661 aaataacaaa gaatattcaa gtactataga tataataaaa attaatccag aaaataaaaa
      721 aatcaagact ataaaaagta taaatttaag taattatttt tccgaaaaat accaaaacat
      781 agagaacata ggtgtaagtt atgctagcaa atatgaggat aagatgtata ttttagtaaa
      841 tgtactaaat acaaacatag gtgataatag taattctaaa tttcatttac ttgagtataa
      901 tcttgaagaa aataaggaaa gcataatgga attaaaaact gatagcaaaa tggctttagc
      961 aaaagcaaag ttagaagcta ataaattata tatggtaatg tcgaatgatt atgataatag
     1021 ttcaattaca gagttaaatt tacttaccta taatatatta gataaaagct tagatgaaga
     1081 taaatatgtt ttagataata gagaaaaaag aggaattaga agtattgaaa ttgattcaaa
     1141 taaaatcgtt gcatatacat caaggctaaa ctctatatta tcttgggatg ataaatttat
     1201 gctatatata ataaataaaa attctaagga aattgtatat gaaggagaat tacaatttaa
     1261 cagttcaaat agtatgttag aatttgaaat aaagtaggtg agttgatgaa agatttaata
     1321 agattattcg aatttgaatt aaaaagaaat ataaagaatt atatatttat aataatttgt
     1381 tgttgttctt ttgtaatttt aaacatagta aaaaatttaa atgactataa ttacataata
     1441 gaaaatgcag ttaaaagtga acagttaaca aaaatcggaa atacgataga aacggtaaat
     1501 gttacaggtt tctcaagctt tagaaatata atgggttcta cagaattgtg gtttatgttt
     1561 ggtataattg catgtatttg ctatgctttt tttatttggt atagagactt taatggtaga
     1621 agtaaatcta tatatacact tataatgcta cctaaaaata gaatcaatat ttatatatct
     1681 aaattattaa atatattatt tctagtgtat agctatacag tagtacttac aatatctttg
     1741 tttattgcaa gtaagttgtt acctagacat atgcttggta atgtcacaaa ctatggtttt
     1801 gtacaagaaa caatatatga gttaaaaatg ttacttccat atagctttga aatactattt
     1861 gtagagtata tatttttatt gataggattt gtatctgtag tatttacatc aattcttata
     1921 aataaatcta tttataaagt aagcacttta ataagttttc tatttttagt tatagaaatt
     1981 ttagtgttta taatttctat agaatttatt ataccttacg aatacagtga tgtatttact
     2041 gttctatatt caagtataaa catatttgta tgtagtctta tatctaataa acttttgaag
     2101 caaaagattg acttttagga gggatattat gaaagcaaaa aatataggaa aatatacaat
     2161 atgttttata atagtatgtt ttttaatgat tactatgacc ttaatattga gttattttaa
     2221 taaggataaa ctatctgtaa aaactttaaa aggcaatgta aaagatatag ggagagtaag
     2281 tgtagtatat acacctaata taagcaaatt ttcttatgag gaggtaatta taagtaaaaa
     2341 tggagtaaga aataatacaa acccaaaatc aagaattaga aatgattatt taaatattga
     2401 caaaaaatac agtgactttg ttaatgataa aggaaaagaa tttatatata aagatgattt
     2461 gaatatagga gaagcgtata cctctgttaa gtctgattta gtacaaggaa tgaatgcttt
     2521 aaatgattgt ataaaggttt caaataaaaa tttaaaaagt ggggtagaag aaaattttga
     2581 tattaattta aataagtctg attttaaaga aaatagttgg aaaaccttat ttaacaaaat
     2641 atataaaggt aatttatata tggtaattag tgaaactaat atttcaggtt ctaaagtcaa
     2701 taatatttgt atattaaaga tagatataaa aaataagtct tataaattat taaatagatt
     2761 taatttagga ttacagaatg gagaacaaca aaatatcaat gaaattaaat tttctattgg
     2821 aagtaaatta tacttagaaa tagtaaataa taaaagtaaa aatggatata ttgaaggaca
     2881 tgatagttac tctaacttta ttatatatga tatagaagat aacactttta atataagtaa
     2941 taaatttatg gataatctaa acaaagaaaa gaatgcaaat ccaaaggaga ttggcttatt
     3001 aagtaaaact aagctagatt atatggtaaa aaacaataaa ttgaatctta tagttacgga
     3061 tatgagtaat tccataacta atcttgttta tgatattaat tctaatgaga ttaaattaga
     3121 agattatgag atacttgatt taaaagtaga ttataatgaa gcatcatctg gagttaggaa
     3181 agttaagcta ctggatgata agatatattc tatttcaggt attaagaaca gtatatcagg
     3241 agaattaaaa ggatttaaag ggattgttga tgtagtcgga actaagccag taaaatttca
     3301 agtatttgat atgaaactta aaaagaatat ttatgaagct gagctaataa atggagatat
     3361 aagtttacaa gaaaagttat tttttgttgc aaattaagtg ttagaattaa ttttagaact
     3421 ttaaactttt atatgtatca cagataagta taagtaaggg agtgtctcaa aataaacttt
     3481 ttagctcatg agacactttt tttatatgaa atcaaatata ttttcattgt ttagatttat
     3541 tttgataatg aaaaaaagat tatctttatt ttaagaaaga catttattta taattatata
     3601 caaaaatatt ttttaatgtt tttgtgtata attataacaa tttaatttat attaatgcac
     3661 ttaaatttga aatagaatgc ttatagtggt aaaattaatt attagaatat tagaaataat
     3721 aaataaataa tacattgtac ttaaaagggg gagaaattta agatgaacaa aaaatatatt
     3781 aaagcaggga atttaattga tggaaccgga aatagtatta tgcaaaataa gggtataatt
     3841 attgatggag atactatagt tgaaattgaa gatataagag atgatttaaa tggatatgat
     3901 gtgtacgatt acttagataa aactgttatg ccaggcatta tgaattgtca tgttcacttg
     3961 acaatggagc cagttggaaa tccaaagata tattatgata atgtatcaga tgtagagctt
     4021 gtagtaaata cgataaaaca acttgatgct tatttggatt caggagtaac atatattaga
     4081 agcttaggtt gtcccaaata tgtggatgta caacttaaaa atcttataga caaagggtct
     4141 atagatggtc ctggtatagt tacttcaggt ccagttatat gtatgacagg agggcatgga
     4201 tattattttg gtattgaaag tgatggagtg gatgaatgta gaaaatcagc cagaacagtt
     4261 ttaaaaaatg gagtagattg tgtaaagata atggcaactg gaggtgtaac aactgatgga
     4321 gttgagccag ggtcaccaca attaactttg gaggaaatga aggctgctgt agatgaagct
     4381 ataaaagcag gtaaaataac tgccacacat gcacaaggaa gaactggtat aaaaaatgca
     4441 gtgcttgcag gaattacttc tatagaacat ggtgtttatt tagatgatga aataattgat
     4501 ttgatgcttg aaagaggaac ttacttagtt ccaactgtgg ctgcaccata ttttattcta
     4561 aatgcaggaa aagaaagtgg aataactgaa gctactctta gaaagtgtga gattgttgcc
     4621 attccacata aagagtcatt cctaaaagca tataaaaaag gaattaaaat tgcagttggt
     4681 tctgatgcag gaacttctta taatgaacat ggaaaaacat attatgaaat gaaactaatg
     4741 tcagattatg gtatggataa tatggatata atagttgctg ctacaaaaac agcatctgaa
     4801 cttttaagaa ttgataaaaa ttatggaact ctagaaaaag gaaagaaagc agatgttata
     4861 gttgtaaatg gaaatccagt agagaatata gacgtacttg cagatgtgtt agcagtattt
     4921 aaacttggta aaaaagttag ataaatagta taaaaataaa aaagagtgtc taaagaaatt
     4981 tttaaataat tatagacact cttttttatt agtaataatt aaatctttta tactttagaa
     5041 aatagattct attttatatt tagatataac tctttaaaat cattcttatg caatataatc
     5101 tatagaatct acacatacaa agtatattct tgaatcatta gaatttccaa gtactataac
     5161 atcatttttc ttacctagaa cttcaacacc agttagaacc aaactaccag ctactacagt
     5221 aacttgatta tttatatttg acatataaag attatctagg atacctttat tacaacagca
     5281 atcatcacaa tcgcagttgc aattacattc tttgtaatgt tttttatcaa gatagtaacc
     5341 taaagatgct atgaaatctt cataatctgc aggtgtaata tcaaatgcaa cagctttaag
     5401 attacacaat gatgctaaat ctacgtttgc taatggagta gatgcagcaa gtataaaatc
     5461 aattatagca gcaagtattg catctaatat agccccaagg tctatcgtag gaggaataac
     5521 tgcatcaata agtgcaatta attcagctac tcctggtata gttcctatag tattaattaa
     5581 tccctcaaga gttaaaggga ctggaatagg ataagatact ctaccagcta tatctattaa
     5641 atcacagtta caagcagaaa atctttcaaa agtaccatca agtccagata aattatcttt
     5701 tggaggagct gaaagatcta tacctaccaa attagcacct actataaaga aatctgagat
     5761 aaaagcaaat tgattaaagt ttacaaaagg tcttagagca tcatatctta gaagttctaa
     5821 tgcttctctc atactttttt tacaacaaca ttgttttatt ttaggttctt tatatttatt
     5881 tggataatta tagtatctat cagctacttg ataattttca tagtatcttt catttgtatt
     5941 tggataatct tcctcatatt cttgtgtaaa tctaaagtcc tctctacatt tattattttc
     6001 cactaaatac actcctttta tattttaatt taaatgtata gataattaag cttatctata
     6061 tagtaatatt atatttataa tgtaaaaaaa tgttacaaaa gatattataa acgaataaaa
     6121 aataataatt tgccagataa acttgtaaat ttttatgaat aatccattct aaaacaaaaa
     6181 actgtgtata aaaaaggttt atatgttata tttatataaa tataacatat gaacgagaaa
     6241 ggagtggttt gataaatgat tgaggtagat aaattgtgta agtcttttac aagggttgta
     6301 aaagatgata agaataaaag ttcaataaga aagctaaaga aagtaaaaac taaaaaagaa
     6361 gaattttttg ctgtaaataa tgtttcattt aaagttatgg aaggtgaaat tgttggaata
     6421 ttaggtccaa atggtgcagg aaagactaca ttacttagaa tgttaggtgg catattaaca
     6481 ccaacatcag gaagtataaa tatttctgga tatgattatt caatagatag aaattcagca
     6541 aaaaaagaaa taggttatct ctctggaaat acaaaactat atggaaggct ttctccgaga
     6601 gaacttctaa caacttttgc aagtctatat gagatgtcaa aagaagatat agaagaatca
     6661 attgaaaata ttgtaaaaat aatggatatg agtgaattta tagataatag aattgaaaat
     6721 ctatcaactg gtcagactca aagaacatct atagctcgtt gtttaataca ttctccaaaa
     6781 gtatatattt ttgatgagcc aacactaggt ctggatgttt taagtagtgc atctattata
     6841 gattttatga agaatgaaaa actaagggga aaaactgttc tttactcaac tcattatatg
     6901 gaagaagcag aaactttatg tgacaagatt tttatgattc acaatgggaa aataattgca
     6961 tcaggaacac cacagagttt aaaagaggaa tctggagtaa ataatttgag agatgtattc
     7021 ataaaacttg ccagaaagga ggacatttaa atgagaagta agattgtaaa atacatattt
     7081 aaaaaagaaa tgttagacat attgagagat aaaaagactt tatttatgat gattgttgtg
     7141 ccacttcttt tatatccttt acttatgtta ttaatgattc aagtaatgaa tatgtctgca
     7201 agtagtatga atagtaagga cattaatata gcttttaata ataaacctaa taaggtttta
     7261 gtatccatga ttgaagatga taatttagaa aaagatacta aaaatacttc aaatgataaa
     7321 ggcaaaataa gagtgttaga tgtagataac tataaaaagt cattagaaga aaataaaatt
     7381 gatgcttata taaagatgga agaaaaaaaa tcatctataa attatcaaat atatataaat
     7441 tcttctcaag aaaattcttc taatgcacta aaaagaattg aaactgtttt ggataaatat
     7501 aaaagattta ccatagaaaa aactttatct gaaaaaggat tagatgtaca agctacttta
     7561 gaaccaataa catatagcac tgtagatgtt gcaaagacgg aagaagtggc aggatacttt
     7621 ttaggtcaga tacttccatt tatattaata ataggagttt tactaggttc aatatatcca
     7681 gcaatagatg ttatggctgg agaaaaggaa agaggtacat tagaaacatt atttacactg
     7741 ccaatatcaa acttagaact ggttatggga aaatatatgg cagtttctct tagtgcaata
     7801 gtaacagcaa ttttaaacat tttatctatg ggactgacta tggcatttat tttagttagt
     7861 ggaggtatta gtagccaatt tggctttgga aactttaatt atggagactt agtcttacca
     7921 attataacta ctttaatttg catatgtctt ttttcaatgg tagtttcagc aataagtatg
     7981 tgtgtatgtt cattagcaaa aagttttaaa gatgctcaaa actacataac acctcttatg
     8041 ttaattatta tgataccatc atatgtttct atgataccaa atatagtatt agatagagtg
     8101 acagctgtaa tacctgtagt aaatatatct ttacttataa aaagtgtatt atcttttaaa
     8161 agcgatttaa atttaatagc cttagtttta gcatcaaatg tagcctttgt tatattggca
     8221 atcatactac tgtctaagat gttcaattct gaggaaatac tttttggtaa tagtaagagt
     8281 ttctctttct tagagaaaag aagtaatata aaaaaagggt ctataccaac tgtaagtgat
     8341 ggtgttattt tatatgcagt aggattagtt ctattgattt atgttggttc tttagttcaa
     8401 attaaatttg gtatggcagg tattataatg acacaattca tgataatttc tttaccgtta
     8461 ttatttgcat actatattaa agctgatttt aaagaggtat ttagcttgaa agttcccaaa
     8521 ttaaagcatg tgtttgggag cattgttctt tggattggtg gatacatact tattatgatt
     8581 ataacacagc taattttatt cttatttcct caaaatatgg aagttgctga aagtttaaat
     8641 aaggctcttt ttatcaaaga tagtgtttta ctgaatttac tagcaatagc tttattacca
     8701 gctttatgtg aagaaatgtt ttttagggga tttatatttt cttcatttag taaatcaaaa
     8761 gataaaaata agtctatcaa gctagctata atatgcagtg gggtactatt tggtattatg
     8821 catatggatt ttattagaat aattccaaca tcaatattag gtattatatt tgcatatagt
     8881 gtgtataaat ctggttcaat atttgtatca atgttattgc attttttaaa taatagtgta
     8941 gcagttgtgt taaatcatta tacaactgga aatataacca acttatataa atttgttgaa
     9001 gttgattttt cgaacttaaa tgtacttaaa tttgccttaa ttcttctaat aagtcttata
     9061 ttaattatgt taggattaag agttttagat agaaagtctt taagaaatta agtataaagt
     9121 tattatatag atta
//
LOCUS       DAMKMI010000148         9070 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMKMI010000148 DAMKMI010000000
VERSION     DAMKMI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.154"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(101..835)
                     /locus_tag="PC429_003184"
     CDS             complement(101..835)
                     /locus_tag="PC429_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF3848662.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
     gene            960..1676
                     /locus_tag="PC429_003185"
     CDS             960..1676
                     /locus_tag="PC429_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3848663.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            1810..2502
                     /locus_tag="PC429_003186"
     CDS             1810..2502
                     /locus_tag="PC429_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848664.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            2492..3673
                     /locus_tag="PC429_003187"
     CDS             2492..3673
                     /locus_tag="PC429_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3848665.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            3955..4410
                     /locus_tag="PC429_003188"
     CDS             3955..4410
                     /locus_tag="PC429_003188"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF3848666.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            4400..5125
                     /locus_tag="PC429_003189"
     CDS             4400..5125
                     /locus_tag="PC429_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF3848667.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKYVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            5097..7478
                     /locus_tag="PC429_003190"
     CDS             5097..7478
                     /locus_tag="PC429_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF3848668.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(7683..8912)
                     /locus_tag="PC429_003191"
     CDS             complement(7683..8912)
                     /locus_tag="PC429_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF3848669.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWNIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
BASE COUNT         3553 a         1052 c         1535 g         2930 t
ORIGIN      
        1 ataataacat ataataaata aaaaagtaag attgtgtaga ttatataaag attatttaca
       61 attaatacaa tcttgctttt ttatttaaaa attttaatta ttatttaaaa ttttttatct
      121 tctctttatc ccatataaac aatttctcag aattatttaa agcaccaaat acatttcttt
      181 taaaatcagg catctcttta tttaaattag caataagatt ttttatgtac tctctcctag
      241 tatgaccatt tgcagggcag ggatttgtga taataggata gttagaatct tttgaaattt
      301 tcttagtcat atactcttct atgtaaacca taggtcttat aactgttaaa tcctgtctgt
      361 ctaggtatgt ctttggggaa aaacaattta ctctaccctc ataaaacata gacattaaaa
      421 atgtctcaat tgcatcatct ttatgatgac caagagctac tttattacat ccaagttttt
      481 tagcattatc gttcaaagca cctcttctta aattagcaca caatgcacaa ggattctttt
      541 cttgtcttat atcaaataca atttctttta tgtcagtttg aaattcataa aaaggaacat
      601 ttatttcttt acacaattta tctagtggag aattatcaac acctccagta tttaatgtta
      661 tggctatcaa ttcaaagttt tgtggagaaa atctttgata acttttaagt atatgaagta
      721 gagttaaact atcttttcca ccagaaagtc caaccgcaat tttatcattt tcttgtatca
      781 tatcaaaatc ttgtatagcc ttacgagctt tacttagaag tttttgtata gacattttat
      841 caccatcctt taaaaatatt aaatttaaga tttatttaag atttacttat aaaatactta
      901 agtcttatac tctaaaatta acattatagc aaacataaca aaaaggagaa gatttaatta
      961 tgaaaaaatt taaattaata ggagccatta ttttagtagt atttataagt gtatttatat
     1021 ctaaagatag attacttaag aaagataaag atccaatagt agctgctcaa catgattcag
     1081 aacaagatgt tgaggcaaat tctcaaaata aaaatcactc aaatagtata tcaaatatat
     1141 tattggtaaa caagacaaat ggaattagca aaaactatac tcctgaaaat attactaagg
     1201 taaacatacc ttttgttgaa gaagcaacag aggaagaaaa acaaatggca ggagaacctg
     1261 caaaggcagt tgaagattta gttaagcaag ctaattcaga aggaatacag tttttagggt
     1321 cttcagcata cagatcttat gatactcaac tggatactta tacaagaagg gtaaaatctc
     1381 aggggcgaga aaaggcagat gcatatgtag caaagcctgg ttacagtgag caccaaactg
     1441 gactttgtat tgatttaaca aatccagaaa gatggtttgt tggaagtaca aaagaagcta
     1501 aatggttggc agaaaatgct cacaagtttg gatttataat aagatatcct gaaggtaaag
     1561 aagatataac aggtacagca tatgaaccat ggcatattag atatgttggt aaggatgctg
     1621 ctgaggaaat ttattcaaaa ggattaactt tagaagaata tttacaaaat aaataatata
     1681 aatgtgtaaa taatttgtat tatattggaa atttagaaat taataaattt atagattaca
     1741 ctaactaaaa tatagtataa ttttatatga ataaagctat atacaaagat taagttagga
     1801 tgtgtaaaaa tgaataatac agttcttgtt gtggatgatg aaaaggaaat aagagacctt
     1861 atagagatat atcttttaaa tagtggatat aatgtaatta aagctgaaga tggaaaagag
     1921 gctctagata ttttaaaaaa ggaaaatatt catttaatga tattggatat aatgatgcct
     1981 aaaatggatg ggatagaagt ttgtagaaaa gttagagaaa gtttgaatat accaatactt
     2041 atgttaagtg caaaatcaga agacatggat aaaatacaag ggataatgac tggtgcagat
     2101 gactatttga caaaaccatt taacccactt gaacttactg tgagagtgaa gtcgctgatt
     2161 agaagagcat attattttag tggtgctaat aatgaagata aagatgtcat aaaggttgga
     2221 gtagtaatta tagataagga aaaacatagt gtaacagtgg ctggaaatga aataattttg
     2281 acatcaagag aatttgatat attatattta ctagcaaata ataaaggcag agtatatagt
     2341 acagaagaaa tatttgaaaa ggtatggaaa gagaaatatt accaatctaa taatactgta
     2401 atggttcata tgagcagaat aagggataaa attgaaaaat atacagatgg agaaaaaata
     2461 attcatactg tatggggagt aggatataaa attgaaaaat aaaagtttat taaatcatat
     2521 gtttcgaccc atattagatc ttataataag ctgtgcttta acttcaatgc ttattttaac
     2581 aagtatgtat ataataaaat cattatatgt caatagtgat atgtctgtag ttaatcaact
     2641 tgtatatcaa gcaagagatt tcagaagtct tatagggtat gatttatttt atccattttt
     2701 atttacaatc acatcattag caatttattc cattatatcg tacaagagaa ataagcaaat
     2761 tgtagatttt ataagaaaga ctaatagaaa aaatcaaaaa ttagaaatca ttaaaagtgc
     2821 tgtaaattta atggatgaag gtaacttaga aaaaagtata gatattgagt atgatttaga
     2881 tatattaaag gataaagaaa aggatgatat tgatgaacta gcacataaaa tcaataatat
     2941 agtccatcaa cttagaaata taactattga agagagacaa gctcagcaga caaaaacaga
     3001 tttaataaca aatgtgtcac atgacttaag aacacctttg acatccataa tgggttattt
     3061 aggagtgata gaagaaggta agtataaaga tgaagttcag atgatgtact atgttgatat
     3121 tgcatatgaa aaatcgaaaa atttaaatgt tcttataaat gatttgtttg aattgactaa
     3181 aatgcaaaat aatacaataa aattaaacaa aatagaaata aatttagtag agttattaag
     3241 tcaagtagta tctcaatttg aaatttattt taaacaagaa tttatggtta gtagaattaa
     3301 ttttaatgaa gaaaaattaa ttgtaaaagc tgatccaaat aaattggtaa gagcatttga
     3361 aaatcttata actaatgctg tgaaatatgg aaaagatggg cattatgtag atatagtcac
     3421 agagaaaaag gaagatatgg cggttgtaaa agtgataaat tatggggaac caattccagt
     3481 gttagattta ccaaatatat ttgatagatt ttatagagtt gaaaaatcga gaaatagaaa
     3541 tgttggaggt tctggattgg gtcttgcgat tacaaaaaat attgtaaatt tacatgatgg
     3601 agaaattact gtttcaagcg ataaatatca aactgtgttt gaagtacaac tgccaataat
     3661 acaatcagct tagttataaa ataaaagtac gattatgtga tataaatatt ataacaataa
     3721 agtgtttaca aaaataaaaa agtaatatat aatttaagtc agtttaataa gatacttatc
     3781 aagagttagg ggagagagtt agctcagtga tcctatacca accttttatc aggtggtcct
     3841 gctaacaacg atgagatgac ttgaaataat atactcttct tatcaataat agataggaag
     3901 agtttttttt agaattaatg taacaaattt taaagtataa tggaggatta aataatggaa
     3961 aaagtagaaa gttttaagct agaccataca aaagtaaaag ctccttttgt aagaaagtgt
     4021 agtgtacttg atggagtaaa gggagataaa gtaactaaat ttgacttgag atttttacag
     4081 ccaaatgtag aaagttttgg aactgctgct atgcacggtt tagagcatct tttagctact
     4141 tatttaagag atacattaga tggtgtaata gacttatctc caatgggatg tagaacagga
     4201 ttttatttaa tactatgggg agatgtagat gccaaaacag ttaagattgg tttagaagaa
     4261 gcattaaaaa aagttcttga atcagataaa atgccagcag ctacagctat agaatgtgga
     4321 aactacagag acttatcttt atttggggca aaggaatatg caaaagatgt tttagataaa
     4381 ggattttctt taaatatata tggagaataa tttaattgga agagaagata taagcataaa
     4441 tcaaagtgaa gttttaagat atttacaata cagaggtcaa gagatagata aagaccttaa
     4501 atatactatt gatgaatgtg tagctataac taaaaataaa ataaatccta gatatttatc
     4561 taggatttat cctattagaa taaataaaga taatgttgta gaattagagg gaacaaattt
     4621 aacattagag agtaaatatg tatatgaact tttaaaagaa tgtaatgagt gtgtaattat
     4681 ggcagctact atagggatag atatagaaaa agaaataaga cgacactctt attcaaattt
     4741 aacaaaagga ctaataattg attcatgtgg aacaacagca atagaagaag tgtgtgacat
     4801 tgttcaaaat aatttagaaa aggttttttt aaagcaagat aaatttataa ctatgcgata
     4861 tagcccagga tatggagatt taccaataga aaaaaatata gatatattaa acgttttgaa
     4921 tgctcaaaaa caaataggtc taacaattac ttctaatgga attatgatac cgagaaagtc
     4981 agtagtggct attataggta tttctaatac aagaataaat aaatctaaga aagaaaagtc
     5041 gtgtgaaaat tgtaaaaact atagaaactg tacatataga aagggagcga actcttgtgg
     5101 aaataagaca atatctgaaa aataatatat taatatttga tggtgcaatg ggaacaatgc
     5161 ttcaacaaaa agggttaaag ttaggtgaaa atccagaagt atttggtctt caaaatccag
     5221 ataaacttat agaaatacat acagcatact tagaggcagg ttcgaatgta attcttacaa
     5281 atacttttgg atgtaatgaa ctaaaattag atagtaagta tactgtagaa gaagtgattg
     5341 acaatgctgt tttggtggct agaaaagcta tagaaaatgt agataatact aaaccaagat
     5401 atgtagcttt agatattggt cctataggag aaatgctaga gcctatgggt actttaagtt
     5461 ttgataaagc atatgaaata tttaaaagac aagtactaca aggcgttaaa tctggagttg
     5521 atgtaatagt aatagagact atgatggatt tatatgaagc taaagtagct gtattagcag
     5581 caaaagaaaa ctctgattta cctattttct gtactatgac atttgatgaa ggtggaagaa
     5641 gttttactgg atgtatgcca gaatgtatgg tggctactat agaaggtcta ggtgtagatg
     5701 caataggagt taattgttca ttaggaccta aacaactttt accaatagtt gaaaaaatag
     5761 catcaagagc tacagtccct gttatggttc aagcaaatgc aggtcttcca aatatagttg
     5821 atggagaggc tatatatgat gtcgatgcaa aagaattttt tgaaggggtt aaaaagttcg
     5881 ttgaagttgg tgctactata ataggtggat gttgtggtac gaatccaagt tttataaaag
     5941 aaatatctga gaatataaat tctgttacaa agggatgtat cgagaaaatt gataaatgtg
     6001 tagtctgttc accatcaaaa tttgtggaag tacaatcgcc tacagttgtt ggagaaagat
     6061 tgaatcccac aggtagaaaa tctctacaag aagcattaaa aaatgaaaat gttgattatg
     6121 ctataaattt aggtttagag caagtaaatg ctggagcaca aatacttgga gtaaatgttg
     6181 gtcttccaga gattgatgaa aaaaaactaa tgccaaaatt aataagagag attcaagcag
     6241 tagtagatac acctttacaa gttgattctt ccaatgtaga ggccttagag caaggactta
     6301 gatattataa tggtagaaca atagtaaact ctgtaaatgg caaagaggag tctttagaaa
     6361 gtatattacc aatagttaaa aagtatggtt catgtgtggt tggacttaca cttgatgaaa
     6421 aggggatacc taaaaaggct gaagaaagat ttgaaatagc aaaaaggata gttaatagag
     6481 cagtttctta tgggataaaa ccaaaagata tatttataga ctgtttatca ttaactgttt
     6541 cagcacaaca agaagaagca atagaaacta taaaagctat aacaatggta aagacactag
     6601 gagtaaaaac tatattaggt gtttcaaata tttcatttgg tcttccaaat aggaaagcac
     6661 ttaatgcttc ttttctaaca ttagctttag gggcagggct tgatttagca attataaatc
     6721 caaatgaata tagtatgatg gaagctatca attcttttaa aatattaaat aacacagata
     6781 aaggatgtat aaactatata aatcaatata gcaatataaa taattcaaaa aaatcggatt
     6841 catctacaaa gattgataaa gatttgccct tggatatttt agttgaaaga ggattaaaag
     6901 atgaagcaaa aaatgtaaca ctgaatcttt taaaagagaa ggatgaaaat tatatattag
     6961 atgagatttt aattcctgct cttgataaag taggaaaaag atatgatagt ggagatatat
     7021 ttttaccaca attgattcaa tcagcagaaa cggtaaaagt ttcattaaat acaataaagg
     7081 aaactttatt aagtaaaagt agcaataatg tatccaaagg aaaaataata gttgctacag
     7141 taaaaggtga tatccatgat attgggaaaa atatagtaaa aataatgcta gaaaattatg
     7201 gatatgaagt tatagattta ggtaaagatg tccctattga agaagtagtg gaagttgcta
     7261 aaaagagaaa tataaaatta gtaggattaa gtgcacttat gactacgact gttcaaagta
     7321 tgaaagatac aattcaggca ctgagagata atgagataaa tgcaaaggta tttgtaggag
     7381 gtgcagtttt gacagaagaa tacgctgaag aaatgggtgc tgattattat tcaaaggatg
     7441 caaagtctgc tgttgaaata gcaaagttaa atttttagca tataaattaa aataaaggtt
     7501 ttactaaata ggatatttta tatataatat aaatataagt tttagatact tatgtttaaa
     7561 acaataggaa ttaatctata ataatgagat taattaatat aaaaaatatt tatcatcaac
     7621 attaaaaagg gctgggaagt ctggtataac ttccatcagc cctttttagt taaaatcata
     7681 tgttaaaaag cccaattacc atttttaaaa atctgtattt gctttccatc ataagtctca
     7741 ccaataatat ccatatcagc agaaccaatc ataaaatcta catgtgttaa actgtcattt
     7801 acaccatgtt catccaattc ttcatctttt aaattatttc caccttccag acaagtctta
     7861 taagcagaac caattgcaaa gtggcaagaa gcattttcat catatagagt attataaaaa
     7921 attatatccg tattggatat tggtgagttg tatggaacaa gagcgacttc tcctaaataa
     7981 tgagagcctt catccgtttc aataagtttt tctagtgtat ctagtccctt ttcagcagag
     8041 aaatctatta ttttaccatc tttaaatgtt aagaagaaat tgtcaattat gtttccacca
     8101 taaacaagag gttttgtact ataaacagtt ccatttactt taaacttatg tggcattcca
     8161 aaaatctctt cagttggaat attcggatta aaacttattc cattagggtc tttggatgct
     8221 ccacttagcc aaacatgacc ctttggcaag tctagagtca aatcagtttt tgaggattta
     8281 tattttaaag ttttaaagtt atttttattt agaaagtcca ttttagattt aagattttca
     8341 ttatgttctt cccatgcttt tatagggtcc tgaccatcta ctcttgaaca tttaaatata
     8401 gcatcccaca gtttttctat tgctttatct tctgataaat ttggaaacac tttttttgcc
     8461 caatcttttg ttggcacaga gactatattc catttacatt tatcagttag agtgtaagaa
     8521 cgccattctt ttaaagcttt tccagatgcc ttctgaaaat ttgcaactct ttcagggtca
     8581 acatctttta ataaatctgg attttgggca tagactgata aaaatgcccc accttctttt
     8641 gctatatcta catattgttc tgctgtccat ttggggaact catgaaatgc ttcatctgga
     8701 gcatataagt atttaataag agtactttct tcatcagacc actctatata aacattttta
     8761 gcaccacatt tatatgcttc tttaagtgca tttcttgcaa aatcagcaca ttctattggt
     8821 gactttataa gtagaatttg acctggctgt atgttaactc caacttttat tgctaattca
     8881 gcgtattttt ctaaatttct atttaaattc atatttatcc tcctatattt ttaatattga
     8941 ataacttata aagtattata acattactta tgtttttaat attgaacaac ttataaaata
     9001 ttataacttt acttagattt tgggtcatat tcaaaatatt tatactttat agattgagaa
     9061 ataaaaaaaa
//
LOCUS       DAMKMI010000149         8991 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMKMI010000149 DAMKMI010000000
VERSION     DAMKMI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.155"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(71..679)
                     /locus_tag="PC429_003192"
     CDS             complement(71..679)
                     /locus_tag="PC429_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF3848670.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(788..3526)
                     /locus_tag="PC429_003193"
     CDS             complement(788..3526)
                     /locus_tag="PC429_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF3848671.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINVCISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3674..4465)
                     /locus_tag="PC429_003194"
     CDS             complement(3674..4465)
                     /locus_tag="PC429_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3848672.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4501..5157)
                     /locus_tag="PC429_003195"
     CDS             complement(4501..5157)
                     /locus_tag="PC429_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848673.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5150..6115)
                     /locus_tag="PC429_003196"
     CDS             complement(5150..6115)
                     /locus_tag="PC429_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF3848674.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKNLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISNKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6221..6327)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6617..7561)
                     /locus_tag="PC429_003197"
     CDS             complement(6617..7561)
                     /locus_tag="PC429_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF3848675.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNEFTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSASIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATVIEPIELKHLIKSKVLELYNHHCI"
     gene            7672..8139
                     /locus_tag="PC429_003198"
     CDS             7672..8139
                     /locus_tag="PC429_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848676.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8309..8908)
                     /locus_tag="PC429_003199"
     CDS             complement(8309..8908)
                     /locus_tag="PC429_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848677.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2917 a         1491 c          871 g         3712 t
ORIGIN      
        1 tctataaaaa aataagatac atacctataa aataccttcc tatagataca tatcttacct
       61 atataaaaac ttattctata taaaaacgcc ataaaaaatc ttttgcttct tctgcataat
      121 ctatatttat acgtttacta gtttttatat ctaactcatc tttactaaat actttttttc
      181 ctttatcatc ataataaaaa tcacttatat ataattcttc acccattata ctcttactat
      241 ttagacttct atctattttt aaagctttgc aaagttttcc tggtccgtta gtaatatttt
      301 ttacttgata tttactcaac tcagtgtaag ctttgttgta tctattcata gatatctcat
      361 ctaaagaagt tataggctct acccctctta ttaatacaca ctctggcaca ttttctatat
      421 ttgcagataa attcaagcaa ttatacatac cataaattaa ataaacatat atatgtccac
      481 catctaaata taatggctta gttctatctg tctttttatt gccatataca tgtgctcctt
      541 tatcatttac acccatgtat gcttctgtct ctatgatttt ggtaacaatt actttatttt
      601 catatttcct tattagatat ttacctaata ttgatttggc taaatcaatt ccattttttc
      661 taaaaaaatc cttttccaaa atttcacctc ataaaattta tttaaaacgc atcaaataca
      721 actaaagtaa taaataaaat taactcttat taatgtgata tatataaaca ataaatttag
      781 acatttatta tttatatata tcaaaaaatt ctatctcaaa cttttcaata ttatcattgt
      841 aatttgaatt atcatatact tcttcaacaa cctctttaat tgggaatata acttttacat
      901 tagttccttc tcctacctta ctatctatgt aaatcttccc attttgaatt tcaatcaatg
      961 attttgtaag gcataaacct ataccacttc caaattcatt tgatatacca ctgttttcaa
     1021 tttgtttaaa tcgattaaat atatcattta atttttcttt gtctatgccc actccagtat
     1081 cttttataat tatctctact gtttcgtctt ttttatatat acctacaagg atttctcctc
     1141 cttctaaagt aaattttata gcatttgata atatatttag tattattctt tctatcgatt
     1201 ctgggtcaag ccctaccata acagtttctt catctgtatc aaaaattaaa tctattcctt
     1261 tagacttagc ataactacta atagatgtaa ctattctttc tgttatgtca ataatatcaa
     1321 aattaactgc tttaatctta tatatgtctg acttaacttg agaaatattt attatattat
     1381 ctattaatct caataatcta aacatatttt gtttagttat ttttatatgt tcttttagta
     1441 tatcctgaaa atcaattatt tttccttttt tataaaggtt ttcaataagt tgaactgaac
     1501 tatatatatt atttaaaggt gtcttaagtt catgagatat attagcataa aactccatct
     1561 tcatttggtc atactgtctt tgttttatta atgctttttt aagtctttca gactctcttt
     1621 gcacacttaa atctttaata attctcactc tacttctttt ctgtccaaag tataatgttc
     1681 ttgatatcat ttttacttct ttaatgtgat tattttcatt tactatatgg atactcttct
     1741 ctctttctcc tatatttaaa ttgtttatgt atttttttac taatatattt ctatattttt
     1801 gtgttgtaac tcctaataca tcactagaaa ttccgaacaa ctctctaaag gctctatttg
     1861 tatatagtac tcttgatgta cttaaatcct caagaaaaat agcctctggt ataacatcca
     1921 gtaatttttc atatattatt ttttgttcat ttactgttct aagaatattt atctctttag
     1981 ttatatctct aaatgtaagc aatttaaaga gtttattatc tgaccaatag tctccaactc
     2041 taacttcttc aacatgctct tcaccttctg agtcagacat cacaaaatta actatacagt
     2101 ttgctgtgtc attgtaactt actatttcag ctttcttttc ttgtgttaga tttaatcttt
     2161 taaattccct attgtcaaag aaatctatgt taaaagtttc aaaatattgt tctaatgtca
     2221 tatcgactaa atcacttgaa acacatttaa aactcttttc atatttttta tttatactta
     2281 ttaccttatt cttctttatg ccaccttcag cacttcttac aagtatagga gatggaatat
     2341 ttagtataat gttttcatag tctttattta tatttcttat ttcttcatct attttgattt
     2401 catttgtaat atcatttaaa cttattaagt aattgctttc atctttatta ttacttaaac
     2461 ttaacaaata aattttatca tttaactcta ccagtttatc aattatactt atatcgttat
     2521 caaatttctt caatttttca gcaacttttt catcttcctt tataaaagtt aatattttat
     2581 aataattatt catatccttt gaaaattcat ctttaaattt attatttgtg taacttatat
     2641 taaaatcata gtctgttact atccatacat ttggtattgt atccagagct tgtctaagta
     2701 attcttttct ctggtctatg tagtttttta atgttctttg tataatcata tccttttcta
     2761 ttgctaaatt tatctttcct aattgctctg actttttact taatttttta ttgattgaat
     2821 caagttcata atatggatac actactctat tattaagact gtatacatat acataataag
     2881 tatggttaca taatagtaaa tatattactc ctattaatct atctgaatct actatattaa
     2941 tcttagaaat tgctacaaaa catataatag ttgtaaaaaa aacaaaatat gttaaatctt
     3001 ttattaactt atagtgctca atgttactat caagtttatt caacaaatat agtaaattaa
     3061 acatagatat acaaacatta attaatataa taatcatatt ccagttaata tcactaaaaa
     3121 tatttcttat aaagtaatct aatatcacaa aagcactata aaaaattagt attgggctta
     3181 ttcttagttt ttttacttta gaaatggtta agtatataaa agtagacata ttaattatgc
     3241 ttaagttaag caatacaatt tggaattttt gaatttttaa atttactact cctaaaatac
     3301 ttacaaaaac taatacaaga caccctactt tcaatatatt tataaattca tctgttttaa
     3361 agctacttat ataaactata gaatatccaa atatccaaag taaaattatt ctatacatat
     3421 ttatagaaac tgtatacaaa tttatattta aaagtgcaat aaccaaaaat aacccattag
     3481 ctagtatata gaccttattc ttacttaaag ttcctttaga ttccatattc cccaccactt
     3541 cttcattttt atatagtttt acctataaat atgttaccac aattcttcca aaaaatttgt
     3601 cactttctag ttataaaaaa aaatgagtaa atatttttaa ttttactcat tttgtagatt
     3661 ttgtatattc agtttaaaaa gctggaacta tacttccttt atattcatct tgtataaatt
     3721 ttttaacttc tttactattc atagcctctg ataaagcctt aatcttatca ctatctttat
     3781 tattttctct acaagctatt atatttacat atggagaatc acttgattct attactattg
     3841 catccttagt aggatttaag ttagctgtta aagcataatt agaatttatt actgctccgt
     3901 caacatcttt taatactgtt ggtaattgct ctgcattcat ttcttttatt actatatttt
     3961 taggattttt agttatatct tttttagtta taagctctcc atcttttact tctatcaatt
     4021 tattttttgc taataattta agtgctctag ctccattagt tgcatcattt ggcacagcta
     4081 taacagctcc atcttttaat tcatctaatg acttaacttt ttctgaatag aatcccattg
     4141 gctctatatg aactttagat gtataagtaa gtttatatcc tttctccttc acagtttcct
     4201 ctaaaaatgg aatatgttga aagaagttag catccaaaga cccttcatct aaagctgtat
     4261 ttggaagtac ataatcatca aatattttta cttctaaatc atatcctttt tcttttaaca
     4321 aaggttttgc aacttccaat atctttgcat gtggatttga tgaagctcct actagtattt
     4381 ttttatcctc tttgtttgaa caacctactg ctgatattgc tattgctgac accaatgcta
     4441 ctgataataa tttctttaat ttcattttta ctccccctct ttaaaatgtt tataattaat
     4501 ttattttagt ttcttataag ccagatttcc tactccctgg attatctgaa ctaatattat
     4561 aagtgctatt acagtatata ccattactgc tgtatcaaat ctttgatatc cataaataag
     4621 tgctatatta cctaatcctc cagctcctac tgcacctgcc atagctgtat aaccaagtat
     4681 agatattatt gataatgtaa ttccagatac tattgaaggc atagcttcct ttatcataac
     4741 tttaaatact atttgtcttt ttgtagctcc aaaagatttt gctgcttcta tcaatccttt
     4801 atcaacttca tttaatgagc tttctataat tctagcgata aacggtgctg ccccaatagt
     4861 tattggcact attgcagcag tttcccctat acttgttcca acgattaatc tagttattgg
     4921 aatcattgca actataagta tcataaatgg gaaacttcta aatatattta ctataaatcc
     4981 taatgcacta tttattgtgc tatttggttt taatccatct ggttttgtaa ctactaatat
     5041 gattgctaat ataaatccta atattgttgc cactatagtt ggtactataa ccatatacaa
     5101 agtttgtaac aatgcatttg gaaatagtgt tgttaaaaag tcaattaaac tattcattta
     5161 tcatttcctc ccatcttatg cccttactag ttagatattg ttttacttgc tctccacttt
     5221 tatttgaaat gtttataata agtgaaccta aaatatcatc tttaaattgt tctaatttcc
     5281 caaatattat agacacatct atatttagtt ctcttgccat agtcgtaata atagcttcat
     5341 tagaaatatc tttagggaat agtatcttta tatttgttcc ttttggcaat attatacttt
     5401 cttcacctat taattttcta agtcctttag tatttcttaa gaatatttct tctgtatctc
     5461 ctacctctaa tacttctcca ttttccatta ttgctactct tccacaaatc tgctttataa
     5521 cttccatttg atgagttaca actattattg ttattcccaa gtttttattt atatcttcaa
     5581 gcaatgaaag aattgacttt gtagtatttg ggtctaatgc tgaagttgct tcatcacaaa
     5641 gaagtacttg tggatttagt gctaaagctc tagcaattgc aaccctttgt ttttgtccgc
     5701 cacttagatt tctgggttta tcatttttct tttcagatat tccaactact tctaataact
     5761 ctaaaactct ttttttaatt tctgcctttg agtatccaaa acattctaat ggcaatgcta
     5821 cattatcaaa tacagttttt ctttcaagca atgaaaagtg ttgaaaaatc attcctaact
     5881 cttttcttaa atctctcatt tgcttttcat tcaatgactt tacttcttta tcagaaacaa
     5941 gtacactgcc ttcttggtat tcttctaatc cgtttataca tctcagtagt gttgatttcc
     6001 ctgcaccact atgacctatt attccaaaaa tttctccaga ttctatttca atacttacat
     6061 ctttaagcac ttgaatcttt ccataatact tattaacatt ttttatgcta atcataaaat
     6121 ttcctcccat atattctgct cagtttttat gtttatcctc tcttatgtat tacaacaaaa
     6181 taaaagcccg agtataaact caggcttaga aagctattta tactcatcta tcaactatat
     6241 tagttgcagg atttagcacc aatgtatata aaatatactg gttgccgggt ttcaaagggc
     6301 cagtccctcc accactcttg ataagacgtt atttaattta attctattat catttgttgc
     6361 tatgttatat atgatatatt cattaatagt atttgtcaat aaatttattt aaatttttta
     6421 ttttttatta tcgttaatta actattttta attagttttc tagctcctaa cactttatat
     6481 tttaatgtct atataactat agaacatcat tataagaaat aaattaaata acatcaatct
     6541 atctaaatca tctgtaaagt aaattaagaa aattaacatt ttatccaaac tctatcctac
     6601 tattaaataa aataatttaa atacaatgat gattatatag ctctagtact ttagacttta
     6661 tcaaatgttt aagttctata ggttctatta ctgtagcatt tttcccaaaa gttaaaaaat
     6721 attcagccac ccagttaagt gtagattttg gtatatacat atctatgtat ccacctttag
     6781 acaagatttt caaccctctt gccaacaaaa attctgtttc acaacgtttt actccctcat
     6841 ctgtcaaatt aattttaata tgataatctg tacctacttc catattctta agataatcat
     6901 gtatagaaga taaaacctta tatttcttac ttgataagtt ttctatactc aaaatcttca
     6961 caattctgtc tacacgaaat tccttaatag atgctgattt tatacagtaa gctggacaat
     7021 accaaagtcc attcatggca tacaaaccaa taggaacaat ggtacgtaca ctattttttt
     7081 gctttgatga atattcaatt ttaattgtaa attcattcat tactatatta aacaattctt
     7141 tcaataatgg tgttgaacag tgcctatctg gtgtccaaaa aacaaattta cgccttatat
     7201 tctcaatatt atattgaata tcatttggta tacaatttaa aaattttttt aaaacagaaa
     7261 tagtttcttg ctcaaatggc aaatcattat aatattctag tgactgatat gcaaagaata
     7321 tagattttac ctcactttct gaaaatgcaa tcggtggaag tatacgctct ttaagtacat
     7381 gatatccacc agccgctcca acttttgaat ataatggaaa tccaatactc tccagcacct
     7441 gtaagtctct tagtatagta cgttttgata cagaaaattc ttctgctaat tctcctgctg
     7501 taaaacttct ttttgcattt attgttatca tcagttcaat taaacgctca gctcttgaca
     7561 tatttatact cctaatttaa atatagtgac aataattgtc actattatat gataagatat
     7621 tcttacattt aaagcaataa taatagtaaa gaaagaaggg actgatactt tatggaaaat
     7681 aatactatta aggctgatga tattatcaat tattgtctat caaacttaga tgatgtttta
     7741 ttaatgaata gttgggggga acgagcaatt tactacaatc ctaatggtat tttaaagcga
     7801 ggagtatatg ttcttaccat taaagaaaaa gacagtaatc atgataaagg ttcgttggtt
     7861 agtcgcccaa atgtataccg tgtgaatata ggattaaaaa aagaaacttt tattgaaatg
     7921 tttggatata ttccaaagcg tccaggtgta ggtcaaatag ttgatatgga ttttgatttt
     7981 acaaaattgg acacaatcat gccacatcct atctactcat ggatgggatg gatatgcgtc
     8041 ctaagcccta ctgaaaagac atttgagaac tttaaaactt taataggaga atcttacaat
     8101 tttgcaaaac aaaaatttaa aaagagaaaa aacaagtaaa aacgtatgaa aataatccct
     8161 aaataaatta taataaaaat attaaggtta caagataaaa attaatatct taataacatg
     8221 caaaaaagcg aagccaaaaa atgtattatt tataaggctt cgctatatct caaataattt
     8281 atttcaaatt tcaaaactat ttattctatt atttcttatt atttcctgtt attcctaatc
     8341 tatcattcat atcttttatc atttttctaa aaccactacc tatatcattt ccaacatctt
     8401 cagttccatg aactaatttt ataaagtcat tttgaccttt tttcatatct tcatcagata
     8461 ccttatctaa tttttctatt ctcttatcta tgtcttttac taatctttcc ccttcagcga
     8521 ttaggtcatc attcattttc tttaactctg tatcttctgt tttaccatct tttttaagag
     8581 atgttacaaa tgattgtatt ttatctctac tatctttata tgcagccttt acatcactta
     8641 gatatttttc atttcctgga tattctttat ctttaaactg attatttata ctttctggag
     8701 tatcataaat agaatatcta tttagacctg ctatataatc attatagctt gttgaataat
     8761 aattcatata tccttcatta ttatttacag attcattagg attttttcct gtattttcac
     8821 ttggtttttc attattttta gtacatgcag ttatacctat agctaatact agtgctaatc
     8881 ctacaaataa agttcttttt aatttcattt ttattaccct cctagatgtt ttatactata
     8941 aaaaatttta ataattttct ttcttaattt agtatttcta tatttttata t
//
LOCUS       DAMKMI010000150         8713 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMKMI010000150 DAMKMI010000000
VERSION     DAMKMI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.156"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            428..682
                     /locus_tag="PC429_003200"
     CDS             428..682
                     /locus_tag="PC429_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848678.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            718..1041
                     /locus_tag="PC429_003201"
     CDS             718..1041
                     /locus_tag="PC429_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848679.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIF
                     IDYII"
     gene            1315..1467
                     /locus_tag="PC429_003202"
     CDS             1315..1467
                     /locus_tag="PC429_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848680.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            1661..2584
                     /locus_tag="PC429_003203"
     CDS             1661..2584
                     /locus_tag="PC429_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF3848681.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   2851..3531
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2851..2878
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            3723..3875
                     /locus_tag="PC429_003204"
     CDS             3723..3875
                     /locus_tag="PC429_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848682.1"
                     /translation="MKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNLTKKEWLEEKIDE
                     ELEQKE"
     gene            3995..4809
                     /locus_tag="PC429_003205"
                     /pseudo
     CDS             3995..4809
                     /locus_tag="PC429_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            4861..5022
                     /locus_tag="PC429_003206"
     CDS             4861..5022
                     /locus_tag="PC429_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848683.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5351..5815)
                     /locus_tag="PC429_003207"
     CDS             complement(5351..5815)
                     /locus_tag="PC429_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848684.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(5951..6268)
                     /locus_tag="PC429_003208"
     CDS             complement(5951..6268)
                     /locus_tag="PC429_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848685.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(6551..6847)
                     /locus_tag="PC429_003209"
     CDS             complement(6551..6847)
                     /locus_tag="PC429_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848686.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(6866..7279)
                     /locus_tag="PC429_003210"
     CDS             complement(6866..7279)
                     /locus_tag="PC429_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848687.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7263..7496)
                     /locus_tag="PC429_003211"
     CDS             complement(7263..7496)
                     /locus_tag="PC429_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848688.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            7715..8089
                     /locus_tag="PC429_003212"
     CDS             7715..8089
                     /locus_tag="PC429_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3848689.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            8379..8713
                     /locus_tag="PC429_003213"
                     /pseudo
     CDS             8379..8713
                     /locus_tag="PC429_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3524 a          918 c         1375 g         2896 t
ORIGIN      
        1 tgtaagaacc gtaggaacta cggggatagc ctgtggagaa ttagtaagac atatgttagt
       61 atgcaaaatt ctgtgaagca ggaaacctgt gactttagtc atgggaggtt caggctagta
      121 ttactcatta ttgtattcaa aagctaaagt ggaaacctag tgaatatatg aatttagaag
      181 ttaatgagag agcattagca gccgactcaa tacttattaa aattgaagat gaagaagaaa
      241 caatgaaaga agctgaaaga gagaaaaaga gaggtagaag aaggtagaat ttaaagttaa
      301 aatagttaca ttgctaatat atgaaaatgt tacaaatttg gaaatagatg gatatatatg
      361 ttgtatagta taatgtagat aatatatcac caagtaaaaa acaacaaata aatctaaatt
      421 atgaggggtg gaaaataaag atattaaact tacaatatgt atatttgctt ttgtaattgg
      481 aataatagtg ttattttgct gtataggtct aggtcaattt aatatggatt gtattataaa
      541 acaaaatggt gggtcaatag ctaccgaaca atacaacata tatttagaac aatctatttc
      601 acaatataga aattttggtt ctatactggt tttactagga ggaataggaa tactgtttga
      661 taaaagtgtc agaaaatact aaaaaagtat atttttattt aggatgtgat gaatgatatg
      721 gcaagagatg tatttagtgt aaagatgaca ccactagaag cacagaaaat ggtagaaaaa
      781 aatttggaag cagatttagt ttctgttgat atttataacc tagaaaataa taagtctata
      841 atagttacaa tctttgaaaa atattatgca agaagcaata gtgatgcagg tttggtagtg
      901 atttgtgaaa atactacagg aaaaacactt gttaaaatta cctcaactgg tagtgcaatt
      961 ggactatttc agattgattg gggaacaggt aataatttga ttaaaagagt aaaaaaaata
     1021 tttattgatt atattattta attattttat gtagatattc ttaggttggt atggaatttg
     1081 tactaaccta ttaagtaatc aataaaacta aataaacata gaaagcactt acaaatatgt
     1141 aggtgctttt tattgaaaaa atagaaaata aaataaaaag taagatatag gaaaaatatg
     1201 taagaattat atgttataat aattgtagca agaagatgta atctacaatt tatagagtgg
     1261 agttcatgta ttaaaaaatt atcctcccaa cgataagaag ggaggtgaat atacatggat
     1321 aattttttat ttaatgtgtt agctagtgta acagctagtt acatagttta tttgattagc
     1381 aagttaatca aaaaagtaaa aagccactct accgcaaaga gtgacttaaa gattgatata
     1441 aattttaaat atcatcgtaa aaaataactt tcagttatag aacttcactc taccgcaaat
     1501 agattgtagt tcttcttgtt tttattatac cacaaattag aaaaaatatt gcatataata
     1561 ttttttatag tcaataaaaa tatgaaattt tttatacaca atgatgatga atctaactag
     1621 atgaataaat ttaattgtaa ataagaaagt acttacttaa atggtaggtg cttttgtttt
     1681 gccaaattat gctataatat tgagtgagat gagtaatttg tgggggaatt ttataatgtg
     1741 gatattagta ccaataataa caatagtatt acttataata gctgtttctt ctatgcaata
     1801 tattttagtt atgatagctt ttttgttgat tatatattct tttatagaaa agaaaatagt
     1861 tatgggtctt gtgtcagttc tttttttcac ttattcaatt tatctttgtg caacttggga
     1921 agataaatcc ttgatagcag ataataaagt tgagactgtt aaggcacaaa gagaaactgt
     1981 agaaagagaa aaagaaatgg aaagaagaag aatacaagaa gaagtagaca aagaaagata
     2041 tattgaaaaa catggaatgg aaatatcaga aaatgattta aaagttaagc tagaggcttt
     2101 agtacctcaa gaatataagg ggaagaaata tgaattaaaa gtaggaaaat ttaaaagata
     2161 tagtatgtat tttgatttaa ctgtacaaaa tgaaaaattt agtaattctg aagaatgtaa
     2221 aaaatttgta aaagaaattg ctaatgatct taaaaaaatt aaaataagca aggcgtactt
     2281 taagtttcat tcaaaagatg acggcggtat atataattct gtttatattg attattttag
     2341 aaacatacaa aataatgtag ataatgttga aaatttagaa ttcaatgagt ttgaacttaa
     2401 aaccgaagaa gaagagaagc gagaacaaga gaaaatagaa caagaaaaaa acagttataa
     2461 taactacatt caaaacagag tggttgaccc actagataga ataaagaaac taaaagagtt
     2521 gctagattca ggagcaatta cacaggaaga atataataaa aagaaaaaag aattattaga
     2581 atagataata tagtaagcac ttacaagtat gtaggtgctt ttaaatttac aaagtattcc
     2641 attttaattt tatagtttag attttatgat ataataaaaa tatagaagtt ttgcagtgtg
     2701 cgatatttgt tacaaagtag ggcttaatac ttgaaatcta agatgttgag ggtgcgtgat
     2761 aagtgttatc aattgcacta ttgcccgctc actgcaattt taagagtatt gtatatatgt
     2821 aggtattgga aatgctaagt ttattttggg gttttagatt aactatatgg aatgtaaaac
     2881 tgactcggga gcatataata agtaatatca agaatagttt tagattaact atatggaatg
     2941 taaaattcgt aaaataacaa ttgtcaggaa tatgagaagt ttgttttaga ttaactatat
     3001 ggaatgtaaa attggactca aaatataagt taaaaaaatt tttaagtttt agattaacta
     3061 tatggaatgt aaaaattttt gacgctatga agaaatttac tacaaaattt gttttagatt
     3121 aactatatgg aatgtaaaaa cttttttcat cctcccaaac ctcttgttcg ctgtcgtttt
     3181 agattaacta tatggaatgt aaaaaagaaa ataagtttaa aaaaaggttt cattattata
     3241 tcgttttaga ttaactatat ggaatgtaaa accaattatg tacagtttag ttactgtgtt
     3301 aagtcttgtt ttagattaac tatatggaat gtaaaatcct tttataaata tcttctgaca
     3361 tgtgtatatg cttgttttag attaactata tggaatgtaa atcggctaca acggtataaa
     3421 ttaaatttag cagggtacat tttagattaa ctatatggaa tgtaaattgc tgtgatttca
     3481 ataataaaac aattaataat tgtgttttag attaactatg tggattcaaa attaaataaa
     3541 caaagaaagc acttacaaat atgtaggtgc ttttattctg ctcaaaattg gtcggttggg
     3601 taaaataatt agaaaaagtt agtaaaaacc tattgactgt aactcgttac aatattgtta
     3661 ttaatgtaac gagttacaga aaagaggtga ataaaatagc aactaaaagt agggcagagt
     3721 atatgaaaaa tcgtcgaaaa gataaaagag gttttagtgt acttttagac aaagaaaagt
     3781 tagataaatt tgatgaagtg ttagaggaaa agaatctaac caagaaagaa tggctagaag
     3841 aaaaaatcga cgaggaactg gaacaaaagg aataaaaaat aagggtcact cccaccgacc
     3901 aaagtttgag taacccctat gacgtatact atcgtatatc aattatagta tatgtcattc
     3961 cttaaaaaaa tcaattatta aggagtgtaa tattatgaaa aatgaattaa tgatgtttga
     4021 aggaaaagag attgaagtat ttgaatttga aggaagaatt ttattcaact caaaagatgt
     4081 agtaaactgt ttagacataa aaaatgttaa tgagaatata agacttatga atgaaaagca
     4141 agttgtaaag ttaagaaatt cggatatcag taatactgac atccgaaaac ttaataatgc
     4201 tggagaaaat tttcttactg aaagtggagt gtataaatta atctttaaat caagaaaaga
     4261 agaagctgag agattccaag gctggataag tgatgaagtt ttaccaagca taagacaaac
     4321 tggtgcatac ataaataata atgcaatacc gaaaagttga gagaaaaagc aagtgagatt
     4381 gaaaaattac agttggttta taacagtaca tctatgttaa aagaattgct agatgatgca
     4441 ggctttgaca ataaatcaaa actattaaca gctaaaacat tatataagaa agcaggaatt
     4501 gatttaccaa tagagataaa cgaagaagaa cattattttg atacaaaaca aatagcatct
     4561 aaactgaaaa tatattctaa gagtaataaa ccagctcaga tggcagtttg tgagattatt
     4621 aaaaagattg atttagaaga tggtgaagtt aaaggtgttt gggaaattaa tggttcttgg
     4681 actggtactg taaataaata tacaaagagt gtaatagata aggtgagaac ttggatagag
     4741 gaaaataata gacctaccaa gattgcaggt gagaagaaga attattatgt attttacaaa
     4801 attgagtaaa tttatcagtt gtattaaata atttagttta gttttggggg gattaataca
     4861 atgtgtgaga atttacttga taatatgtat attgaaaaga gaaaagaaga atatagaatt
     4921 aaacttttga aaataagaga aacagatata gatatatata ataagttaga aagtatagta
     4981 tataaacttt ctgagaaaaa attagagaaa aataataaat agacatataa agcacttgga
     5041 tattctactg tttcaagtgc tttgtttggt ataaaatggt ataatggaga tagagaatta
     5101 tattaaccca aaaatggaga aaatagagaa gatgatttag ttgacgcttt atcagtagga
     5161 gtacttgcag gtaagacaga atctctagta ctattagttg gaaatggaat aagtgatact
     5221 caaaaaagtt taataaataa aagagaattt agaaatataa ctcaaattgg tggaaatgga
     5281 aatgagaaag catttagtga agtagaaaac ttagttaaat agattagaag gtagcaacta
     5341 gagttggttg ttaccttctt tttttatact ttctctctct aagtattttc tttttcatat
     5401 cttctaatac atctaatgca gtagtataaa attgtatatt atttctataa ggtatagcta
     5461 ttggaagaat gcccttgtta actaaaaaaa taagaagtac aataaataaa agtgtcataa
     5521 tcattctaat aaaataatta gtgatatctg ataaatcaaa tgttaagtta attgttgtag
     5581 aaaatacaac agccataata gatatataca aagcaaaaat ggggttacta tttttttgta
     5641 actgtccttt aagcctagat tgctctattt ctatatcttg aatacttttt atggaatcca
     5701 tttcactata atacttataa acttttaaat aatatttttt atacttgttt aaagcttctt
     5761 cattatcttt aaggtctttg tcacttaact ctgatttaac ttccccaaaa tccataaaat
     5821 ccctcctaca atgttatata atatacttta atttatagca tatttaagaa cttatatcag
     5881 caatttgtcg aacgatttta gctaaaaata gagtataatg ataggcttat tatttataag
     5941 atattatttt ttatatagtt atatatttct ttctaattag ataatataat gctgttgtta
     6001 taatggctac taatattatt gctattattg taatatctaa tcttcttgta aataagccta
     6061 ttgctatata ctcaagacct gttacatagc gataaagtac ttctagttta acagtttttt
     6121 tcttatctat atttccatat attgattcta atttattaaa atatttttta ctacaaaaag
     6181 cagttttagg tttaattaaa tatattattg tagttcctat tataactatt attccaataa
     6241 gtataagatt gatatttcca aaatccatag cagatacctc ctattaaatt tgcttttctt
     6301 aatcaaagta tatcagtaac acaagtacaa agagcatcta aaatattaca aatattaaca
     6361 ggatagataa aactatagta aaaattgtaa taataaagcg aacgaaaatt atcatactaa
     6421 aacataagac atgttataat tgtattagat agaagattaa catacaccaa agcactcctt
     6481 acaataaaga gtgcttattt ttttgaaatt catcaatata taaactatca agaaccttat
     6541 tcaacacacc ttaaaatcga tttaaatagc tctttttgat aacataattt atatgatata
     6601 ataaaaaaat agaagtgtac caccaataca cttctatagc tataactaag cattctcttg
     6661 ctgggggagt gcttttttca tttctccaaa caacattatt tacttcatta gtaattatca
     6721 aatcaatctc attatctttc aaaatctctg ttgcagtaca aattagatta tctaagtcaa
     6781 aacttcgagt tatctctagt tgtttgttat ttacttctct ttttagtata taagttttaa
     6841 aatacattta tatcacccct tgtatttact cttttttctc taaaaatata ttgataagta
     6901 aatatgcaac aaataaaaaa atctcaatga cacatagtgt tatagctata actagaagtg
     6961 attgattttt ccatgcacta taagctatga tgaaaagaaa tgtaaaagta aataatgtat
     7021 aattcataat tttaactaag atatgacttg ttttcattgt taagtacaag tctctttcat
     7081 ctgcactatc gtgtaattct tcaacaatgc cttttcttga aaaagcatta caaaaattaa
     7141 agattgctat tacaattaaa agaaatgatg aaattaggaa tcttttatct tttaaaatgc
     7201 aataaataac taaactagca caacataaca tacaaactat aatacccact ataaaacttc
     7261 gtttactctt tactttcatg ttttttatcc tccatttctt tattttcttt taaacaacat
     7321 agttcttcaa tcgttgtacc aaacacttgt gccatacgat aggcaagcat aagagaaggg
     7381 ctatattgct ctttttcaat agaaataatt gttctgtttg atacatggac taaatcagcg
     7441 agttgttgct gtgttagacc catatttgtt cgaaattctc gtacttttgt tttcattcca
     7501 taacctcttt ctatatgaag ctaacttcat gtgaagttag cttcataata tcctaaaatg
     7561 agtaaaatgt caatctcatt agataagtat tataaaacct aaaagaataa tatggtaaaa
     7621 attggatgga attatttacc ttaattctct tgtttgtaaa actttattga tatagaattt
     7681 aacatataca taaatcggca aaggggcgcg aattatgaaa attagtaaat taccagaagc
     7741 ggaattaaaa gttatgagat atatttgtga agctaacaag gtattaactt ctagggaatt
     7801 agttgctgca atggaagaaa aatatgagtg gaatgaatca actacattta cagttttgaa
     7861 aagattagag cgaagggagt ttttaagtac agagaaaata ggcaaactta cacattataa
     7921 tatactagta aaagagaaaa aatatctaag gtttgaaaca aaggaatttt tgaagaacat
     7981 acataaaaat tcaatttcaa gtttaatgtc agcgttacat ggtgaagacg acgaagtgga
     8041 tgaagataaa ttaagtgaac tcgaagaata ttttaagaat ttgaaataag aaaatgacaa
     8101 attgatacat aataaataaa atgaaggtat gaaattataa ctttttctac cttcattttg
     8161 tttattattc tgaacatgtt aagaaaatga aataagtttg tattttattt atataaattt
     8221 taatagaata tgtatagtat atagtagata gaatgtataa aaataaaaac tgttcaataa
     8281 gaattaatat acattaattc agctgaatcc aatcaaaata cttaaataaa agtaatctga
     8341 aaataacata attcaaatca atttattcaa ataaacctca atttatctca aatcttaaga
     8401 agtcattaaa caaacttgct aaattacaaa gagaattatc aagaaaaaca atcggtagtt
     8461 taaatagaaa taaagcaaga ttaaaagttg caagacttca agaacatata gctaatcaaa
     8521 gaaatgattt tctacaaaaa ctatctacta aattaataaa agagaacgat attatttgca
     8581 tagaagattt acaagtaaag aatatgatta ggaatcgtaa actatctcgc tcaatttctg
     8641 atgtgtcatg gtcagagttt attcgtcaat tagaatacaa ggctaattgg catggtagac
     8701 aaattgtaaa ggt
//
LOCUS       DAMKMI010000151         8657 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMKMI010000151 DAMKMI010000000
VERSION     DAMKMI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.157"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            136..486
                     /locus_tag="PC429_003214"
     CDS             136..486
                     /locus_tag="PC429_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF3848690.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            505..2148
                     /locus_tag="PC429_003215"
     CDS             505..2148
                     /locus_tag="PC429_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF3848691.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            2229..4286
                     /gene="recG"
                     /locus_tag="PC429_003216"
     CDS             2229..4286
                     /gene="recG"
                     /locus_tag="PC429_003216"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF3848692.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            4345..4899
                     /gene="rsmD"
                     /locus_tag="PC429_003217"
     CDS             4345..4899
                     /gene="rsmD"
                     /locus_tag="PC429_003217"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF3848693.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            4901..5398
                     /gene="coaD"
                     /locus_tag="PC429_003218"
     CDS             4901..5398
                     /gene="coaD"
                     /locus_tag="PC429_003218"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF3848694.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            5402..5926
                     /locus_tag="PC429_003219"
     CDS             5402..5926
                     /locus_tag="PC429_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848695.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            6270..8189
                     /locus_tag="PC429_003220"
     CDS             6270..8189
                     /locus_tag="PC429_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF3848696.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            8202..8489
                     /locus_tag="PC429_003221"
     CDS             8202..8489
                     /locus_tag="PC429_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF3848697.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
BASE COUNT         3594 a          887 c         1604 g         2572 t
ORIGIN      
        1 aaaaactttt ctaggtttaa attataccat atttatagta tgatatgatt ataattttat
       61 ataataaata agtaaaaata gtaaaaaata aattaagaaa tgtttttaat aaaaataagc
      121 ttaagggggt caaccatgag tgcaaaggta acgaatcaat atggaaatat agaaatagat
      181 aatcaagtaa tagctcaagt cgtatataga gctgcaatgg aaagttatgg cttagtaggt
      241 ttagcatata agtctaaagg aatagtagag ctattaaaag gcgagaacgc aactaaaggt
      301 gtaagagttg aggaagtaga agaagatgcc atagcaatcg aactttatgt tataatacaa
      361 tatggtacaa atatatctac tgttgccaat aatataatag atagagtaaa atatgtagtt
      421 gaaaaaatga ctggcgtaag agttactaga attgatatta atgtacaagg aataagagtt
      481 aagtaatttc ataggaggaa acaaatgatt caatatatag atggtaaaag gttaagggag
      541 atgtttattt caggtgcaaa caatctccaa aacaataaag agttagttga caagttaaat
      601 gtattccctg taccagatgg agatacagga actaatatgt cattaacaat atcttatgcc
      661 ttaaaagaat tagcaaaagt tgaaaatgat aatatatcag atatcggaaa agcattatct
      721 aaaggttctt taatgggagc tagaggaaac tcaggagtta tactttcaca aataatcaga
      781 ggtatagcta aatctataga aggaaaaagt aaattatcaa cagaagattt agcaaaagct
      841 tttaaaaatg gttcagacac agcatataaa gctgtaataa aaccaattga aggaactata
      901 cttactgtag ttagagaaag tggagagttt gcaataaaaa ctgcgaaaaa agagaaagat
      961 gtagtaaagt ttttaagtat gcttgtgaag gaatcaaatt cttcattaga aagaactcca
     1021 gaccttctaa agaatctaaa agaagctggt gtggttgact ctggtggaaa gggtcttgta
     1081 cttatatatg aaggaatgtt agcttctata aaaggcgata acatagaaat aaaaaatgtt
     1141 gacctagata ctaatatttc aacaagtatg gattttgcaa agagtactac tagcacagat
     1201 gatataaaat attgctattg tacagaattt attttagaaa gttctaaggt agaagatact
     1261 aaaataagag acataatgat ggcttatggt gatagcctag cagtagttgg agatgatgga
     1321 gtcataaagg tacatgtaca cactaatgac ccaggaaatg tacttcaaga agctctaaag
     1381 tacggtcaat tattaactat aaaaatagaa aatatgaagt tacaacatga aaatacatta
     1441 ctggatgtag aagaaaagaa agaaaatgat agtgaacctt tagaagaaga aaaagagttt
     1501 gggtttatag ctacttctat gggtgaaggt ttggcaaata tatttaaaga tttcggagtt
     1561 gaccatataa tagaaggtgg tcaaactatg aatccaagta ctgaagattt tatgaatgct
     1621 ataaagcata taaatgctaa aaatatattt attttcccaa ataacagcaa tataataatg
     1681 gctgctaatc aggcaaaaga attgagtgat aaaaatataa tagttatacc tacaaaaaat
     1741 acgcctcaag gatttgctgc attagtaact tttaatggag aattaagcga agatgaaaat
     1801 aaagaagcta tgatgaatgc tttaaactca gttaaatcag gtcaagtaac ttttgctgtt
     1861 agggatacag tgatgaacga aattgatgtt aaagaaggta atataattgg aattgcagaa
     1921 gggaatttat tatcagcagg agattatgtg gatgaagtaa catcaaacct tattgaaaag
     1981 ttggttgatg aagatactgc tataataaca ttattctttg gtgaagatgt tacagaatct
     2041 caagcaaatg aactacgtac ttctttagaa gaaaagtttg aagatgtaga tgtagagtta
     2101 tattatggag gacaacctct ttattattac ttaatttcag ttgaataatg attataagaa
     2161 cctgtatgac caagtagctt ttataaaatt ggttgtacag gatttttgtg tagtaggtga
     2221 tatatttgtt ggatttatat aaagatgttc aatatgttaa gggaatagga cctaaaaaag
     2281 cagataaatt aaataaactg ggtatattta cactaaaaga cctattgtat tattttccaa
     2341 gacaatttga agatagaaat aatctaaaga aaatagcaca attagaagat ggtgagaaag
     2401 ttacgataaa agcagtaata tcaagtataa atacatttag cccaaaagaa ggtatgactt
     2461 taacaaagat tgatgtaaaa gatgagacag gctcggctaa attagtgttt tttaataaga
     2521 gttatataaa aaatactttt cgtccaggag attcaatatt agtttttggg aaggttaaga
     2581 aaaaatttaa taacttagaa cttacatcat gtgaattaga atatcttact aattcaccta
     2641 agaatacatg tagatttatg cctgtgtacc agcttacata cggtgttaca aataaagaaa
     2701 ttatgagtat aattagaaca gtacttgaag ataaagaatt aattatacaa gaatatatgc
     2761 cacaaagaat tatagaaaaa tataggctat gtagtataga ttttgcagtt agaaacatac
     2821 attcaccaag tagtaaggaa tcattaaaaa tagctttata tagaattgta tttgaagaat
     2881 tacttatact acaactaggg ttgtttgtat ttaaaagtgg cagaaataaa gaagatggaa
     2941 taaagtttga aacaagtaaa gacttaaaaa aaataataag tgctttacca tttaaactga
     3001 ctaaagctca aaacagggca ttagatgaaa ttatacaaga tatgaattta gagaaaataa
     3061 tgaatagatt ggtacaaggt gatgtaggga gtggtaaaac agtagtagct ctcttagcac
     3121 ttgcaaattg tgttttaaat ggttatcagg gcgctttgat ggcacctaca gagatattag
     3181 cagggcaaca ttatatttca cttactgagt ctctaaaaga ttttggaata aatgtaggtc
     3241 tattaatagg tagtcttaca aaaaaacaaa aggatacagt attggaacaa ataaagaata
     3301 atgagattga tatattaata ggtacacatg cactaattga agataaggtt gaatttaata
     3361 atattggttt agttataaca gatgaacaac atagatttgg tgttatgcaa aggagcaaac
     3421 tttcattgaa aggagcaaat cctgatatat tagtaatgac agcaacacca ataccaagaa
     3481 ctttagcact tatactctat ggagatttag atatttctat tatagatgaa cttcctccag
     3541 gaagacagcc aatagaaaca attgctattg aaaaaagtaa gcgagataga gcatataata
     3601 accttgtaag aagagaggtt gaatcaggaa gacaagtgta cattgtatgt cccttggtag
     3661 aagaaagtga agccatagaa gccaaatcag cagttgaact tgttgaggag ttaagagcgg
     3721 aatattttca cgacttaagg cttgggttgc ttcatggtaa aatgaagtca agtgaaaaag
     3781 atgaagtaat gagacttttt aaagataaag aaatagatat tttagtttca acaacagtaa
     3841 tagaagttgg tgtcaatgtg ccaaatgcaa cattaatgat aatagaaaat gcagaaagat
     3901 ttggacttgc acaattacat cagcttagag ggcgagttgg aagaggtagt cataaatcgt
     3961 attgtgtact aatttatgat tctaaaacag atgtatgtag acaaagaatg gctataatgg
     4021 aagagaccaa tgatggtttt aagatttcag agaaggattt agaaataaga ggacctgggg
     4081 aattttttgg gacaaggcag catggtcttc cagaacttaa ggtagcaaac ttatttaagc
     4141 atataaaaat actgaagtta gcacagcaag aagctcgata tatattgggt gaagataata
     4201 atctacaatt aaaagaaaac atggcactca aaaaagaaat tatagataaa tttaaagata
     4261 cattgaaaga aatttcatta aattaatttt aaattgtgtt aaaatattac taatgtaata
     4321 atgtgctgaa gggagattgt catattgaga gtaatttcag gaaaagcgag aggattaaaa
     4381 ctaaatactc caaaaaatga agatgtaagg ccaactacag atagagtaaa agagtctcta
     4441 tttaatatga taaattcata tataatggaa agtgaagttc tggatttatt tgctgggact
     4501 ggttctttag gaatagagtg cttatctagg ggtgccaagt catgtacttt tgtagatatc
     4561 agtaaggaaa gcatagatat tgttaagtcc aatataaaaa aggcaagagt tgaaagtgaa
     4621 agtattattt taaaccttga ttttaagact gcaatagata aacttaaatt acaaaatagt
     4681 aaatttgaca taatttttat ggaccctccg tattataaaa atatgtttat agaagctatt
     4741 gaaaaaattg ataattctaa tctattgaac gaagatggaa taatagttgt agaacatgat
     4801 acgaatgatt tatttccaga taagatatgt aaattggaga aaactagaga taagaaatat
     4861 ggaaatacta cattaacttt ttataagatg gaggcataat atggaaaata aacctagaaa
     4921 agctatattt gcaggaagtt ttgacccaat tactaatgga catttagata ttatttgcag
     4981 ggcttcgaag ttatttgacg aattacaaat aggagtatta aataatccta ataaaaaggg
     5041 tctatttagc tttgatgaaa gagtaaaact tatagaaaaa agtacaagcc atttaaataa
     5101 tataaaagtt gtgacttttg atggtttatt aataaattac tgtcaggaaa atgggattgg
     5161 agcattagtt agaggtgtga gaagtggtgc tgatgttgat tatgaacttc agatggcgca
     5221 tatgaataga gagcttaatc cagatataga gactataatt ttaccaagtt gtacaaaata
     5281 ttcatttata agttcatctt taataaaaga ggttttgctc tttgatgctg atataaaaaa
     5341 tttggttcca aagattgttt tggaagaatt aaagaaaaaa actagtgggg gtaattgata
     5401 tatgaagata gatttagaaa tgattgaact atttgagcag ttagaggaga tatttagaag
     5461 tgcttctaca atccctttct cacataaatg cacagtggat aaagatgagg ttttagcaat
     5521 aattaatgat ataagaactt taataccaga agaagtaaca caggcagtat ggattaataa
     5581 agaaagaaat aaaataataa gtcaagctaa gcaagatgct acaaatatag ttgaacaagc
     5641 gaaaaaagaa gctgagagaa tacaacaaga atatcaagat aatatagagg aattaaagaa
     5701 aaattctgaa gatgtagtaa aggcatatgt agaatccagt gaacctgttg ttcaagcaga
     5761 ccaaagagca aaagatatag ttgatagagc tgagagaata gccaatgaga taagaattgg
     5821 gtcaattgaa tatgcagagg atgtgctttc tagtgttgag tataatttaa gagaaatatt
     5881 agaagaagta aaaagagata aagctgaact tagacctaaa aaataaattt taaataaata
     5941 aaaatggagc tatcacacaa aaaattccaa tttatgtgtg atagctctct tttaatatag
     6001 caattttttg ttatataatt gtgcttttta gattacttat atcttatttt gatattataa
     6061 tgtagtttaa tcttttattt gttgaaatca acttatttat ttgtataaat aatataggtt
     6121 attttcaaat ttttttaata attttaccac tgtcttcttt tagattagag ttagactgtg
     6181 caaaattata aaataatact aaatcatgtg caattatttg gtatggcact acatgtagaa
     6241 aatatagata ataaaattaa ggaggtagta tgaaggaaag ggctaaaaaa atattagatt
     6301 ttattattaa aaataacaat gtaacatcac aagaactaca agaaaagttt aatgtttcaa
     6361 agagaactat ttattacgat attctagcta taaatgaaca actaggaaaa agtggtaata
     6421 taaaaaatgt aaaacacaaa tttatatttg aaggtaattt atgtgatgca aggaaaataa
     6481 tttcaactga agaagataaa tttttagata gtgattacag aaaaactttt atattaaaca
     6541 agatattact tggcgaaaaa atatcaatag aaaaactaac aaatgaaatg ttactttcaa
     6601 aaaacacagt agtacaaaca ataactgatg taaaaaaata ccttcaaaca atggggttaa
     6661 gattagagta taagggaaag tacaaaatta taggtgatga atacgttatt agagagctgt
     6721 ttctgataat tgttcaagaa aatgtactgg aaattaattc tataagtgag gaagtaagta
     6781 gtttcgatac aaaaggacat ataaaactta ctgattattc tttattaaat ctaactaagt
     6841 ttgtggaatt tctaaataaa aggataagag atgggaaaac attatactca tataaatatc
     6901 taaatgaagc taaaaaaatt agttacttta gtaattgtaa agaactttta tgtgaagaag
     6961 ctaatgaaaa tgaacaagcc tatatatgca cttacataag ttcacttccc agcttaaata
     7021 gtgaagttaa ggaagatgtt gttgaagaat atgtagataa acttatagat aagtttgaag
     7081 taaatacagc aataaaatta gaaagtaagc atgagtttaa gaaaaatatt ttaaggcacc
     7141 tccactcttc ttacaacaga atacgattta agtttcctat tagaaatcca atgttagatg
     7201 agactaagta taaacatgag tcactttata aaattataaa atcaattata gagaatgaag
     7261 aagagtttcc tgtatttgag gggataagag aagaagaaat aggatttata gcagcgtatt
     7321 ttggaggata tttaagaggg tctagggata atggtctaag aagaaataaa gtcttactag
     7381 tttgtcctaa tggtcttatg gtatcaaaga gtttggaaat acaactatat aaatacatac
     7441 caaccataga aatagtaggt atagtatcca ttaagcaact aaaagaagta aatgtatact
     7501 acgattacat aattacaact atagatatac aaaatgtaaa caacgtaatt gttgtaaatc
     7561 ctcttttaac atcatcagat gttcaattac ttatgaacaa actaataagt gtaaaagaaa
     7621 acgagaagta tttcaatctt gaattaataa tacaagctat aagaaaaaat ggagttatca
     7681 ataatgaaga agcactaaaa gctgatttat taaacataat acacaagata gatgaaggag
     7741 agatgtatca accaatgtta aaggaattaa ttaatgcaga aagggtcaat ataataaaaa
     7801 atgttagaga ttggaaagaa gcaataaaaa tagcctcaaa gccattgtta gaagataact
     7861 ctatagaaga gttatatata gaaaatatga taaagtcagt tgagaaatat ggaccatata
     7921 ttgttttagc tgatagattt gctctaccac atgcaagttc taaagaagga gtaaacaagc
     7981 ttgctatgtc tttactaata gtagaagatg aagttgattt attaggaaaa ccagtaaata
     8041 tatttatggt attagctgct gtagataaca ctactcacat tagagcatta gcatcactat
     8101 cagagatgat gtacgaggaa gaaaatgtaa aactaataat taatggtgat aaaagttcaa
     8161 taatagaact tataaataag caaaattagg gggaatgaaa aatgaagata ttagcagtat
     8221 gtggttttgg tgtaggttca tcaatggtat taaaaatgac aatccaaaag gctctaaagc
     8281 agttaggtat agaatgtgaa gtagaaaata cagatataaa ttcagctaga ggtgctgatt
     8341 gtgactgtat attcacaagt caagaattgt taaatgaatt aaaaggaact tgtaatgtag
     8401 atgtatatcc agttaaaaag tacatggatt tagcagaagt cacagaagtt gttcaaactt
     8461 ttttaagtga taaaaatgat aagaaatagt ataagtttta aaaagtaaat ttaagttaat
     8521 cagaataaaa taaaaactta ctaagttcaa aaatagtaag aaagagagga gggatttagt
     8581 ggtagaaaac ttaaacagtt gacctatcac taaaaaatat aatgcttgat taaaaaatag
     8641 ctacgcacac tcgtgac
//
LOCUS       DAMKMI010000152         8598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMKMI010000152 DAMKMI010000000
VERSION     DAMKMI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.158"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            53..2176
                     /locus_tag="PC429_003222"
     CDS             53..2176
                     /locus_tag="PC429_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3848698.1"
                     /translation="MDSYRQLKAKIPCKETGIEIKRTLCDICSPGVHCGIDAYVKDGK
                     VIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKRIGKRGEGKFKPISWDEAY
                     REIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSFGSPNYGTESSSCSTSAQM
                     AGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLIKLKEKGLKFIIVDPRITP
                     TTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIEKHVHGFNEYKEYVKLFDL
                     DTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQNGFQNYRAMMSLSAITGNY
                     DRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERIGGERFPLWNEMVTQFQAM
                     DLPRQIEEKTPYEIRALFCHGINARMFTNSGRLFKALNSVDFFVNIDLFMTDTSKYAD
                     ILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVEILSDLANAMNIDDDLLKA
                     GYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSYTREGYDTSTSRFELKSTI
                     IEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNALHSRLHDIKLSRILKNEAS
                     CDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLYKNIHMYHGYREADINSII
                     CDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2190..2612
                     /locus_tag="PC429_003223"
     CDS             2190..2612
                     /locus_tag="PC429_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3848699.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3020..3823
                     /locus_tag="PC429_003224"
     CDS             3020..3823
                     /locus_tag="PC429_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF3848700.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3816..4484
                     /locus_tag="PC429_003225"
     CDS             3816..4484
                     /locus_tag="PC429_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF3848701.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4720..5382
                     /locus_tag="PC429_003226"
     CDS             4720..5382
                     /locus_tag="PC429_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF3848702.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5442..6239
                     /locus_tag="PC429_003227"
     CDS             5442..6239
                     /locus_tag="PC429_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3848703.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6357..7547
                     /locus_tag="PC429_003228"
     CDS             6357..7547
                     /locus_tag="PC429_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3848704.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7656..8405
                     /locus_tag="PC429_003229"
     CDS             7656..8405
                     /locus_tag="PC429_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF3848705.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3412 a          862 c         1586 g         2738 t
ORIGIN      
        1 gtataataag tataataatt tgtatttatt attgggaaag gaaaatgtta atatggatag
       61 ttatagacaa cttaaagcta aaataccctg taaggaaaca ggtatagaaa taaagaggac
      121 tttatgtgat atttgttctc caggagtaca ttgtggaatt gatgcatatg ttaaagatgg
      181 taaagtaata aaaatagaag gtacaaaaga acatccaatg aataaagggt tgttatgtac
      241 caaaggtctt tgcaatagag aatatatcta tagagaagat agaataaaaa cacctctaaa
      301 aagaattgga aaaagaggtg aaggaaaatt taaaccaata tcatgggatg aagcttatag
      361 agaaatagca gaaaaattaa atagagtaaa atctgagtat ggagcaaagc atgtagcttt
      421 ttttacgggt tatggaaagt ggtatagacc ttttttacaa aggtttgcat cttcatttgg
      481 ttcacctaat tatggtacag aatctagttc atgttcaaca tcagcacaga tggctggaaa
      541 tgttgctact ggaagaaact ttacaatgca agatatggag aatgcaaaaa cttatttagg
      601 gtggggattt aatccatact actctaaatt tttaaatagt gtgaatctaa taaaattaaa
      661 agaaaaaggt ctaaaattta taattgtaga ccctagaata acaccgacaa ctcaaaaact
      721 agcagattta catttaagaa ttaaacctgg aactgatgga gctttagctc ttggaatggc
      781 gaatctattt atacaaaagg atttaataga tagagaatac atagaaaagc atgtacatgg
      841 atttaatgaa tataaagaat atgtaaaatt atttgattta gatactgtaa gtagaattac
      901 aggagtttct aaagataata tagtaaaagc tgtcgagatg ttgtcacaag gtccattttg
      961 tataaatgaa agtgcagctc caatagttca ccatcaaaat ggatttcaaa attatagagc
     1021 aatgatgtct ttatccgcta taacaggtaa ttatgataga ataggtggaa acataccaaa
     1081 acaagattac tttgctggta agataggtgg attccaaaca ctagaaaaag agtttgtaaa
     1141 agaagttcta cctaaaaata taaatgaaag aattggtgga gaaagatttc ctctatggaa
     1201 tgaaatggtg actcaatttc aagctatgga tttacctagg caaatagaag aaaagacacc
     1261 atatgaaata agagctttgt tttgtcatgg gataaatgct agaatgttta ctaattcagg
     1321 gagacttttt aaagcgttga atagtgtaga tttttttgtg aatatagatt tatttatgac
     1381 agatacatca aaatatgcag atatactttt acctgcatgt acatcatttg agagagaaga
     1441 atgtaagagt tatccaggag ggtatattac atataccaaa aaagttattg ataaattata
     1501 cgattcaaaa tcagatgtag aaatactttc agatttagca aatgctatga atatagatga
     1561 tgatttatta aaagcaggtt atgaagcaag tataagacat atgtttaaaa atacatgtgt
     1621 agatgtagat aaacttaaag aaagtgattt accattaaaa tgtaaagatt ttaagccata
     1681 tatagtaggt tcatatacaa gagaagggta tgatactagc acttctaggt ttgaattaaa
     1741 atctacaatt attgaaaaat ataaagattt tgggctggat gcactaccaa catatagagg
     1801 atttaatgat aatatagatg atgaatatac tcatttattg acatcaggag caagaatttc
     1861 aaatgcacta cattcaagac ttcatgacat caaactttca agaatattaa aaaatgaagc
     1921 tagttgtgat atttctagct tagatgcaaa agaattgggt gttatcagtg gagattatat
     1981 aaaaataact actaagatag gaagtttgga tgtaaaagtc aatgttactg atagagtctt
     2041 atataagaat atacatatgt atcatggata tagagaagct gatataaata gtattatttg
     2101 tgataatttt gacccttata gtggattccc tgcgtataga agttgtaagt gtaagattga
     2161 aaaaatgaat agataaatgg agagatataa tgaaaagttt atttaaattt aataaagaat
     2221 tatgtacagc atgtggagca tgtttgcttg catgtataga ccaaaatgat aataatatag
     2281 acaatggtga actttttaga catgtattta caatagagga aggtgaaaat atagaatatt
     2341 tttcattagc atgtatacat tgtaatgagc caaaatgttt aggtgtatgt cctaaaaatt
     2401 gttttaaaaa agaagaagga ttcattgtat tagacaatca aaactgtata gggtgtaaac
     2461 tttgtgaaaa agcttgtgaa tatggagcct taatttatgg aaatgatggt aaggcaaata
     2521 agtgtaatgg ttgtattgag agatataagt gtggtatgga ttcaccttgt gtaatggtat
     2581 gtcctacagg agcattaaaa tttaataaat aaattaaata tagagaatga cttatactta
     2641 ggtataagat atttttacaa aaagctgtat tgagtttgtt ataatgaaaa ctcaatatag
     2701 cttttttatg gatatagttg gatactataa tgtgattctt agtaatagtt ttttgctatt
     2761 ggacaaagac tgtaaaacat attaaactta tgttgttcaa agtcgaagta taatataata
     2821 actagaaaat tggtttatag tgctataaaa gcaaatttag taaggtataa aatcagaggc
     2881 aatcctttac tataatatca atatttggga gttctgttat tgcagagcta ttaaacaatt
     2941 ataatggtac aagtgattca acctttgaaa atgtattata taaaaaatta aaataagatt
     3001 aaaaaggaga gaataaatta tgaaaaaact taaattaatt attatgtttg ccttggtatc
     3061 agcactgtta gttacaggat gttcatcagg aaataataaa aataaagaag aacaaaaagt
     3121 tataagagtt gcaacatcag gaacatatta cccttttgct tttaaagata aagataaact
     3181 taaagggttt gaagtagatt tttgggatga atttgctaaa cgaactaatt gtaaagttga
     3241 atgggtatta actgatttta gtggattatt tggattatta gaagctgata aagcagatgt
     3301 tgtatctgct caacttactg caacacctga aagagagaaa aaatatgctt tttcagatgc
     3361 ttataattat agtggaacaa acattattgt tagagaagat gatactagta taaaatcaat
     3421 agatgattta gttggcaaga aagtaggtgt gggtactgga gcagtagcaa atgaaatatt
     3481 aaaagaaaaa tacccaaatg atgaaataaa aatagtaaat tattcaagtg cgactcttaa
     3541 agggaattat caagacctag aaatgggaag attagatgca gtagttgcgc aggatgttga
     3601 ggctttaata gctataaaag aacaaaaatt aaatcttaaa atgatagaac ctccaattca
     3661 atttggtgcc tgttcttttg cgcttcaaaa aaatgaaaaa ggtgaaaaat ggacaaaaga
     3721 aattaacgaa gtaattgaag agatgtataa agatgggact ttgactaaaa tatctgaaaa
     3781 ctggctagga aaagacatta ctaaggagcc tattaatgag taattttaat tttgaatttt
     3841 gtctaagtat atttccattt ttgattaaag cattgggaat aacaatacta atatctattg
     3901 ttgcattttt tttatcaata ttattaggtg taatacttgc ctttttcaca cagacaaaat
     3961 caaaagtttt acaatacata acaagagtgt atgtttcatt ctttagagga acaccacttt
     4021 taattcaaat atttttcttt tattttggat ttccaatgat attttctttt atgaaagatt
     4081 gttcacctta tttagcaata gtaatttgtt taggtgtaaa ttcatctgca tatatggctg
     4141 aaattatacg agcatctatt gagagtgttg ataaaggaca acaggaagca tgttatgctt
     4201 ttgggttaag taaatatcag tcaatggtta aaataattct acctcaagca gctacatctg
     4261 cagtacctcc tattgtaagc tgtacactgg acataattaa aggttcttct ttaggtttaa
     4321 ctataggaat acaagattta atgggagtag ctcaagtaga ggctgcttca agttttaaat
     4381 attttgagat atatatgata acgatattaa tttactgggc agtatctata atattgagta
     4441 atatacaaag caaagttgaa aaaagaataa gaagagcata ttagatttca atatttaaaa
     4501 atgggatgat atagatatat tattacttgt aaataaattt ttaattttag aaaaataaac
     4561 ttagtaatct aaattattga gaaaatatag tatcaaaatg gactgtataa tcaagttaaa
     4621 aaatagtttt tctattgaaa aaataaataa aattctattg taatttaata tgcgataaag
     4681 ttttataaaa aacaacatta agctaggggg acttatttta tggcttttaa tatgaaatat
     4741 tttttagaat tatttccaac aataatgaaa tcgttcaatg taactgtgat tttggcaata
     4801 agttcattag tattttcatt aattataggt acaatagtag cgttatcatc ttattacaaa
     4861 gtgaaagtat tgaatcaaat atgtaaagta tatgtatcta tttttagagg tacacctctt
     4921 atgccacagt tattcttttt atattttgga ctagcatata tgagtgattt tgttaaaaat
     4981 atggacccag tatttgcaac atctatagta cttagtttaa atatgggagc ttatatgtct
     5041 gaaacgataa gggcatctat actttcaatt gatacaggtc agatagaagc agcatatgca
     5101 atgggattaa caaatttaca aacaatgaaa aggatagtga taccacaggc cgtaagagtt
     5161 gcactgccat cattattcaa taattttata gatttaatta aagggtcttc tgtagctttt
     5221 gttgttggag taaatgatat aatgggtgct gccaaatcac aaggagcgtt gagttttaag
     5281 ttttttgaag tgtatggagc agttatggta atttactggt gtataataac tcctttaggt
     5341 tgggggcaaa aaaaattaga aaagaaaata agcaattatt aagttattaa gaaaatacat
     5401 aaaagtattt ttatataaaa aatggtgtga ggagaagaga tatgataaaa agaaatatta
     5461 taaaatgttt agtggcagtt ttatcgttaa cgtttgtaat tactggttgt tcaaaaaaag
     5521 aggaagtaaa gaatgaaaaa gttataactg tagcttcatc taattcagct gacccatatt
     5581 cttatgttga taatggtgag cataagggtt ttgaagttga catgtggaaa gagattggta
     5641 aaagaagtgg atataagata aaaatggaac caacaggttt tagttctata tttggtatgt
     5701 tagattctgg taaagttgat gtggcagcca acttctttgg aatgtctaaa gaaagattag
     5761 aaaaatacga tgcttctata ccttatggtt ctgattctgt gtgtatagct gtaaaagatg
     5821 gaaataacga aattaataag cttgaagatt taaagggaaa agttgtagca gtttcagaag
     5881 gttctcaagg tcaacaggtt gcaactagtg ttaataaaga taatttgttt caagaaaagg
     5941 tttacggtga tggaactagt ggtcttcaag atttagattt gggaagagtt agtgcatgga
     6001 ttgatgcaga gcttactatt gtgggagacg ctaaaaaagc taatatgaaa gtaagagtat
     6061 tagataaaaa attatcagct actaatatag catacttctt taagaaaaac gatgataaat
     6121 ctaaaacgat aaaagaagat gtaaacaaag caatagaaga aatgcttgca gatggaacag
     6181 tgaaaaaaat atctgaaaaa tggtttggaa tggatgtaag tgctgatata caaaaataaa
     6241 tactttaaaa ttaaaatgta aaactgtgat aactatagat aagtatacta aatttatcta
     6301 tagttatctg tgtattacaa tgtatttaga ttagtcttta taaatcaaaa tgagttatgg
     6361 aggtattgca aatgattgaa actaaaataa aatccattgc actaaaaaat agagaaaaaa
     6421 ttataaaaat aagacgacag atacacagta atccagagtt agcatttaaa gaatataaaa
     6481 catctaaact tataaaagaa gagctaaata aacttaatat agaatacata aatattgctg
     6541 gtacaggagt attagctact ataaaaggaa aaaataatgg tggaaaaact atattactca
     6601 gagcggatat ggatgctctt ccaatcaaag aggaaaatga tttagaattt aaatcaataa
     6661 atgataatat gcatgcttgt gggcatgatg cacatgtttc atggttgtta ggaacagcaa
     6721 tgattttaaa tgacataaaa gaagaattga atggaaatgt aaaattacta tttcaacctg
     6781 gcgaagaaaa aggtggttct gatattgtaa ttaaagaaaa tgtacttgaa ggtgtggatg
     6841 cattagcgac tggtcattgt tggccaacta ttgagtcagg aaaaattgga atcgctagaa
     6901 attgtgctat ggcagcgact aatacttttg aaataacaat aattggcaag ggtgggcatg
     6961 gagcagagcc tcataattgt atagacccaa ttgcagttgg aaatgcagta tattcatcca
     7021 ttcaacagat agtttcaaga aaaattgacc cagttgtacc tgtagtagtg tctgtttgtt
     7081 cttttaattc tggtgtttct aaaaatataa taccagatgt atgtacactt caagggacta
     7141 ttagagcgat aagcaaggaa caagtaattg aagtaagtaa aattctagaa tatatagtaa
     7201 aaaatgtatg taaagcacat ggagcagatt gcaaatttga aaagagtaca ggaggagatg
     7261 ctgttataaa tgacaaaaac atgatagaga taggaagaga aagtgcttgc aaaatacttg
     7321 gagctgaaaa tgttgaaata attgattttc ctgcaatgac aggtgaggat tttgctattt
     7381 atatgaaaga aaaacctggt ttatttatgt atataggtgt aggagataaa gaaaaaaata
     7441 taaattacag attgcatagt aacaagttta atatagatga aaaatgtctt agtatagcac
     7501 cttcattatt ttcacaactt acagtagatt atttatgtca actatagagt acattcataa
     7561 aaatatattt agcctatttt taaatttaat gctagtatat taattttgat gtagaacata
     7621 ttatttatta tatttattta aaaaggtgga taacaatgat aaaagtagaa aatttacata
     7681 aaagttttaa agggacagaa gttcttaaag gtataaattt agagataagt caaagtgaag
     7741 tagtagctat acttggatca tctggaacag gtaaatctac gttacttaga tgtttaaatt
     7801 atttagttga gcctacaaaa ggaaagataa tgattggtga tgtaaaagta gatgtagaaa
     7861 aagcaacaaa aaaagatata accaatttaa gaaaacatac atctatggtg tttcaaaatt
     7921 ataacttatt taaaaataaa acagcattag aaaatgtaat ggaggcattg atagtagttc
     7981 ataaaaagag taagaaagaa gctagagaaa tatctttaaa attaatagat aaagtaggta
     8041 tgttggatag gaaagatttt tatccatcaa agttgtctgg gggacagcaa caaaggatag
     8101 gtatagcgag agcgatggca gtaaatccaa atgtaatatt atttgatgag cctacatctg
     8161 cacttgaccc agaacttgtt ggagaagttt taagcgttat aaaatctctt gcaaaagaag
     8221 gtataacgat gcttattgta actcacgaaa taaattttgc aaaagaagtt gctgataaaa
     8281 ttatatttat ggataatggt gtaatagctg aagaaggaac tcctgatgaa atatttaata
     8341 atcctaaaaa tgaaagaaca gcaaaatttt taaatatcat taggaaagaa gaagtattta
     8401 gttaaaagtg tataaattaa gcattagaag atatgtaaga tacccccatt aaatttataa
     8461 gttagataat aaatttaatg ggagtatatt tttattatta gatttattat ttaatttaaa
     8521 attagaaatg taactatttg aaaattcttt ttaaaagtat atcaaaatat caaatacata
     8581 aaaaaataat atttgaca
//
LOCUS       DAMKMI010000153         8585 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMKMI010000153 DAMKMI010000000
VERSION     DAMKMI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.159"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            117..572
                     /locus_tag="PC429_003230"
     CDS             117..572
                     /locus_tag="PC429_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF3848706.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            621..1205
                     /locus_tag="PC429_003231"
     CDS             621..1205
                     /locus_tag="PC429_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF3848707.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(1210..1533)
                     /locus_tag="PC429_003232"
     CDS             complement(1210..1533)
                     /locus_tag="PC429_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848708.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            1685..2203
                     /locus_tag="PC429_003233"
     CDS             1685..2203
                     /locus_tag="PC429_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3848709.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(2379..3047)
                     /locus_tag="PC429_003234"
     CDS             complement(2379..3047)
                     /locus_tag="PC429_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF3848710.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            3162..4577
                     /locus_tag="PC429_003235"
     CDS             3162..4577
                     /locus_tag="PC429_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF3848711.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            4800..5258
                     /locus_tag="PC429_003236"
     CDS             4800..5258
                     /locus_tag="PC429_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848712.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            5255..5839
                     /gene="clpP"
                     /locus_tag="PC429_003237"
     CDS             5255..5839
                     /gene="clpP"
                     /locus_tag="PC429_003237"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF3848713.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            6151..7227
                     /locus_tag="PC429_003238"
     CDS             6151..7227
                     /locus_tag="PC429_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3848714.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            7294..>8585
                     /gene="bclA3"
                     /locus_tag="PC429_003239"
     CDS             7294..>8585
                     /gene="bclA3"
                     /locus_tag="PC429_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF3848715.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATG
                     PTGATGNTGADGVAGPTGATGATGNTGADGATGPTGATG"
BASE COUNT         3274 a         1101 c         1584 g         2626 t
ORIGIN      
        1 aaattttaag gaaaaataac atatttgtat taaattttat tgaaatgatt aaatttattt
       61 acttaatgta aattattagc taaaattgta ttaaataatt aaggaggtga gtagctctgg
      121 atgaaataga tttaaaaata attaattctc taaaaaagaa tagtagggct tcaacctctg
      181 aaataagcag acaaattaat ttatcagtac cagcagtctc agagcgtata aggaaaattg
      241 agcagtcaaa tttaattcaa aagtacacta ttcaaattaa tcgggaaaaa acaaaatata
      301 agcttttggc ttttatattt atcaatactg atttatctaa aagtatgaaa gaatttaaga
      361 aaactatatt ggaatatgat actgtattag aatgtcatca tatttcagga gagtatgatt
      421 acttaataaa agtattagca gaagatacaa gtaatcttga agagtttata tcagtgactt
      481 tgaaggaaat tggggggatt caaaaagcta atacaaccat tgttttatct acaattaaag
      541 aagaatttaa tacaataaaa gatatttttt aaataattta ctagtaaagt gttaattata
      601 ttttaggagg aaaatagaat atgaaagttt tagttttttt agcaaaaggt tttgaaacta
      661 tggaatttag tgtatttgta gatgttatgg gatgggctcg taacgattat gggcatgata
      721 ttgatgtagt gacttgtgga tttaaaaaac aagttatgag cacatttaat atacaagtat
      781 tagttgataa gacgatagaa gaagtatgtg ttgatgatta tgatgctcta gctattcctg
      841 gaggatttga agaatttgga ttttatgatg aagcatatga ttcatctttt ttaaatctaa
      901 taagggagtt taattcaaaa gaaaaaataa tcgcttctat ttgtgtggca gcattgccag
      961 ttggaaaaag tggtgtttta aaaaatcgta aagctactac atatcatttg aaaaatggta
     1021 aaagacaaag acagttaagt gagtttgatg taaatgtagt taatgaacca attgttgtag
     1081 ataaaaatat tataacctca tattgtccag aaacagctcc acatgtagct tttaagcttt
     1141 tagaaatgtt gacatctaaa gaacaaatgg atgaagttaa attagcaatg ggatttaaac
     1201 tgtaatggtt tatagatttt ctaagtaatc agaaccccag tcacacatgg aatctaaaat
     1261 tggaataata ctctttccaa aatcagatag cgagtattct acttttggag gaacaacagg
     1321 ataaaccact cttataatta aaccatctga ttctaattca cgtaattgtt gagttaacat
     1381 cttaggcgtt gctttaggaa ttaatttttt taattcattg aacctcaata tagtgtcttt
     1441 aagatgccaa agtataagtg tcttatattt accaccaatc aatgcaagtg ttgcttctat
     1501 tgggcaactg taattatcta aacatttttt catttttggg ctcctttatt tattttaaag
     1561 tatcttttta ggtactatat tacaaaaaag tgcattattg taatatagta ccatagtgat
     1621 aaaattatag cataggttaa ataaatcatc aacttagcct aataaaacaa tagggggtaa
     1681 ataaatgaat ttcgttgaac ttgcaaaaaa aagatattct tgccgtaatt atcaagatag
     1741 aaaggttgaa aaagaaaaac ttgagaaggt tttagatgtt gcaagaatag ctccaactgg
     1801 tggtaatcgt caacctcaaa gattgattgt tattcaggaa aaagaaggta taaataaact
     1861 ttctaaggct gccaatatat atgatgcgcc attagcaatt cttgtatgtg gagataaaga
     1921 caaagtttgg acaagacctt ttgatgggaa acaacttaca gatattgaca caagtatagt
     1981 tacagaccat atgatgttac aggcaactga acttggattg gcaagtgttt gggtgtgcta
     2041 ttttaatcca gatataatta gagaagagtt tagtttgcca gataatctag aaccaataaa
     2101 tatattattg atgggatatg aatctaagat accagagagc ccagagagac atgaaaaaac
     2161 tcgtgttcct ttaagtgaaa ttgtttctta tgaaactctt taattataaa attaagttat
     2221 tcataggata aatgtaataa agtgtttgtt ataaacaaaa ttcaaaataa ataatatcta
     2281 catataatta attttagtga agtatatcta gtttaactag aatgtatact tcactaattt
     2341 atttattcaa aatatttatg aggattttaa tatattaact aaaatatatc aattgaatta
     2401 acttttaaat ctggaaaagc acttgaaata tagtgtttta tatcatttaa atggtcttga
     2461 atttgctttt ctgatatttt atcaggtcca tatacaaaat acataatatt tttagtatct
     2521 tttgttatag ctgttcgatt atcagggcca ttaagaatac tagataatat accaatatta
     2581 tctgataaaa ataaatcatc ttttgttaaa atatgttctt taccacctat accataaaaa
     2641 tgttcacttc catttgctaa ttctattttc aaaggtaatt ccattttatc aaggtcatga
     2701 cctgctgtta gaagaagatt tttcaattct gctaaaaaca ttgactcaat tgttacacct
     2761 acatctggga taccttttga ttttaataga atagattcta attgaagcaa tacagggtaa
     2821 gttttcttga attttttata atactttata taacttgaaa caggctctgt tttaattttt
     2881 tctttgcgat tgtattctgg gtgttgttct ataatatttt taattgtaga gtttttaaga
     2941 actaaaaaat tttctttatt aggttttgaa tgtgtaatat ttataaccat aactccaaat
     3001 ttaatattgg gataaatttc tttaacttta tgtgatacct ctaacataat atagactcct
     3061 ttttatttta tattttatga tataatctta gcaaattaaa agtggatttt atagttccat
     3121 ttttatagta attttataga accacttttg gaggtattag aatgtggatt acgattaata
     3181 gaaatagtaa tatatcatta tcaagacaaa tttattgtga gataaaagat atgattctgg
     3241 atggaagctt aaagtttgag caaaaactgc catcatctag gactttatca aaagaactat
     3301 ctgtatctcg taatacagtt ttagatgcat ataatcaatt aattgcagaa ggatatttgg
     3361 aaacaaggaa tggatatgga acaattgtag ctaaaggaat atcagggcat aaagttgatt
     3421 ttaagttaaa tgatactttt agagatgaaa aagatttaca tactaataaa caaaaaaatt
     3481 atatagattt tcgttcagga gttccagaat taaaactatt tccacgcaaa gaactttcaa
     3541 agctatataa acaaatactg tatgatttat cagattcgga tttcagatat ggaagtacag
     3601 ctggtgtatg ggttttaaga gtggaaatat ctaagtattt atttcgtact agaggaatta
     3661 gatgtaatcc caaaaacatt atgattgttt ctggttcaac acaaggttta tctttaattt
     3721 cacagctttt atacaaaagt aataaaaagg ttgctgtaga agaccctatt cataaaggct
     3781 tattaaaggt tatttcatct attggatatt cagttgttgg aattaaagta gatgataagg
     3841 gaatgaatac caacttaata aaatcaataa aggggatttc ttttgtttat actacacctt
     3901 ctcatcaata cccattagga gggattttac ctattcaaag aagactagag cttattaaat
     3961 gtgcaacaga gaatgattac tatataattg aagatgatta tgatagtgaa tttagatatg
     4021 agggtcaacc aattagttct ctttatgaac ttagcccaaa taaagtaatt tacataggtt
     4081 cttttagtaa aatattatct ccagctattc gtgttggatt tatgttatta ccagacaata
     4141 ttttagaaag ctataaaacc ttaaaaatgt actctgatgt tcatacagaa acaatattgc
     4201 aacatgtatt agctgaattt attggaaatg gaggtttaga gaagtacata tttagaatga
     4261 agaagttgta caatagaaaa cgtaagtacc tcataaaagt tttaaacacg gaatttaaaa
     4321 atgaatttaa aataaaaggt gaagcagcag ggcttcatat agttgctcat ttctataatg
     4381 ttaaatttac ggaaagttta attcataaaa tttttgaagc aggtataaaa gtttatccta
     4441 tacaacaata tgcaattgag aatatattag aatatgacta tgaaattata ctaggttatg
     4501 ctcatcttag ttttgatgaa attgaaaaag gtgtaaaaat actcagcaaa ataatacata
     4561 tgaatcaagg tacatagata aaacatgtag caatctacat ataatagatg taattaagtt
     4621 taaactttaa aagaaatata tattaatatt tttgtataaa aatacctgat atttttttat
     4681 cgatacttta ttgctataca ctaaattaag atagcaattt atattaagaa aaaacttttt
     4741 gcataggtta taatacttaa aaggagagga tagagttgaa aacaaataca gatgttatta
     4801 tggagataat tgattatata gaagaaaatc ttacagaaaa tctaaattta gataaaatag
     4861 cttatattgc taattattca aaataccatt tacacagaat gtttacaaat gttgtaggat
     4921 gtactatcca tcaatacata cagaggagga gacttactga agcagctaga caacttatat
     4981 ttacagacaa atatattata gacatagcta ttacagtagg atattctaca catcaatctt
     5041 ttacactagc atttagaaat ttatataaag aatctccaca agtatataga aataaagatg
     5101 aattttatcc tattcaacag agatttgttt taaatctaaa tcatcaaatt caaagtgaaa
     5161 aatgtatttc ttatgcaaat atgaattata aacaaagaag agatgaaatc aattcttatc
     5221 ttaactttaa tattaattgt gaggtgaaag tagcatgagt ttagtgccac atgtaattga
     5281 acaaacaggt caaggggaaa ggtcttatga tatatattca aaattattaa aagatagaat
     5341 tatatttata ggagaagaaa ttaatgatgc tattgcaagt ttagtagtag ctcaattact
     5401 gtttttagaa tcagaagacc cagattcaga tataattata tatataaata gccctggtgg
     5461 ctctgctaca tcaggatttg ctatatatga tactatgaat tatgtcagat gtgatgtatc
     5521 gactatttgt attggtatgg ctgcaagcat gagtgctttt ttacttgcag gaggaacaca
     5581 tggaaaaaga tgtgctttac ctaattcaga gattatgatt catcagccaa tgggaggagc
     5641 taaaggtcaa gcaacagatg taaaaatagc tgtagataat atattgaaaa taaaagaacg
     5701 attagataaa attctttctg aaaatacagg taaatcaata gaagatatta ggagagatac
     5761 agatagagat aattttatga ctgcactaga ggctaaagag tacgggctga ttgattatat
     5821 aatgaatagg aatgaataaa gtatcaaagt atcatattaa aatgtctgtt ttacaaatat
     5881 atatataata ttaaataaga gtgtctcaaa atgaactttt tagtttatgg gacactcttt
     5941 tttgtatgaa attaaatatg gttttatcat ttcaacaatt attaccaaat ttcaagttta
     6001 ataaattcta ctataaaaaa tacataaaag ataaggttct ttagtaactt tctgtcgtca
     6061 aaagttttta taaatttaat aacaaaacaa gagaaaatac catatattac ttatgtctgc
     6121 aatatataga agacaaagga gttgtacatt atgattacaa taagtttgtg catgattgtt
     6181 aagaatgagg aagaagttat aggcagatgc ttagaatgtg taaaggatat agtagacgaa
     6241 attataattg tagatacagg ttctacagat tccacaaaag aaatagtctc aaaatataca
     6301 gacatggtgt acgatttcga atggatagat gatttctctg ctgcaagaaa tttttcattt
     6361 tctaaagctt ctaaagacta tactatgtgg cttgatgcag atgacataat tttagaagct
     6421 gatagagaaa aattattaaa gattaaagaa agcttggata cttccattga tattgttatg
     6481 gcaaaataca atgtatcctt tgatgaaaat ggtaacccaa ctctctcgta ttatagagaa
     6541 cgattgttta aaagaagttc taatcataaa tgggttgacc ctattcatga agtagtacct
     6601 ctttttggag aggtatttta ttctgacatt gcaatatctc ataaaaagct tcatagacag
     6661 gaccctctaa gaaatcttag aatttttgaa aaaatgattt cagaaggaaa aacattggag
     6721 ccaagacacc aattctatta ttctagagag ttgtactata atgctaggta taaagaagca
     6781 atagaaggtt ttactaagtt tttagactca tcaagaggat ggattgagga ttgtatcagt
     6841 gcttgcagag atttagccac atgttattac ttaattaatg atgaaaaaag tgccttatat
     6901 tcattgttta gaagtttcga atttgatgag ccaagagcag aaatttgttg tgacatagga
     6961 aagcatatgt ttgatagaca aaaatataaa gaagccattt tttggtataa ggtagcatta
     7021 acaagagata agaatgatac taatggaggt tttaagtcaa atgattgtta tggatatata
     7081 ccatctatac aattaagtgt ctgttatgac aggttaggag aatcagataa agctatttat
     7141 tatcatgaaa aaacaaaaga aatcaaacct aacgatagtg ctgttttgca caatgaaaat
     7201 tattttagta aatttaaaaa tagttgatag gtcacaatca tactaaattt gacatattat
     7261 atatagagct taaaagctca aatatatcag gaggtgcttt taataatgag tagaaataaa
     7321 tattttggac catttgatga taatgattac aacaatggct atgataaata tgatgattgt
     7381 aacaatggtc gtgatgatta taatagctgt gattgccatc attgctgtcc accatcatgt
     7441 gtaggtccaa caggcccaat gggtccaaga ggtagaaccg gcccaacagg tccaacgggt
     7501 ccaacaggtc caggagtagg ggcaacaggc ccaacaggac caaccggtcc gactggtcca
     7561 acaggaaata cagggaatac aggagcaaca ggattaagag gtccaacagg agcaacaggg
     7621 gcaacaggcc caacaggagc gacaggagct atagggtttg gagtaacagg tccaacaggc
     7681 ccaacaggag cgacaggagc aacaggagca gatggagtaa caggtccaac aggtccaacg
     7741 ggagcaacag gagcagatgg aataacaggt ccaacaggag caacaggggc aacaggattt
     7801 ggagtaacag gtccaacagg cccaacagga gcaacaggag taggagtaac aggagcaaca
     7861 ggattaatag gtccaacagg agcgacagga acacctggag caacaggtcc aacaggggca
     7921 ataggagcaa caggaatagg aataacaggt ccaacaggag caacaggagc aacaggggca
     7981 gatggagcaa caggagtaac aggcccaaca ggcccaacag gggcaacagg agcagatgga
     8041 gtaacaggcc caacaggagc aacaggagca acaggaatag gaataacagg cccaacaggt
     8101 ccaacaggag caacaggaat agggataaca ggggcaacgg gattaatagg tccaacaggg
     8161 gcaacaggaa cacctggagc aacaggtcca acaggagcaa caggcccaac aggagtagga
     8221 gtaacaggag caacaggagc aacaggggca acaggagcag acggagcaac aggagtaaca
     8281 ggtccaacag gagcaacagg ggcaacagga gcaaatggat tagtaggccc aacaggagcc
     8341 acaggagcag caggaacacc tggagcaaca ggtccaacag gagcaacagg cccaacagga
     8401 gtaggaataa caggagcaac aggggcaaca ggagcgacag gtccaacagg agcagatgga
     8461 gcaacaggtc caacaggagc aacaggaaat acaggagcag atggagtagc aggtccaaca
     8521 ggagcaacag gagcaacagg aaatacagga gcagatggag caacaggtcc aacaggagca
     8581 acagg
//
LOCUS       DAMKMI010000154         8348 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMKMI010000154 DAMKMI010000000
VERSION     DAMKMI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8348
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.160"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            135..1016
                     /locus_tag="PC429_003240"
     CDS             135..1016
                     /locus_tag="PC429_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF3848716.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1077..2285)
                     /locus_tag="PC429_003241"
     CDS             complement(1077..2285)
                     /locus_tag="PC429_003241"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF3848717.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2358..2610)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(2762..3751)
                     /locus_tag="PC429_003242"
     CDS             complement(2762..3751)
                     /locus_tag="PC429_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF3848718.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            3903..4343
                     /locus_tag="PC429_003243"
     CDS             3903..4343
                     /locus_tag="PC429_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848719.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            4670..4897
                     /locus_tag="PC429_003244"
     CDS             4670..4897
                     /locus_tag="PC429_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF3848720.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            4923..7043
                     /gene="feoB"
                     /locus_tag="PC429_003245"
     CDS             4923..7043
                     /gene="feoB"
                     /locus_tag="PC429_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF3848721.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(7176..7994)
                     /locus_tag="PC429_003246"
     CDS             complement(7176..7994)
                     /locus_tag="PC429_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3848722.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
BASE COUNT         2951 a         1206 c         1091 g         3100 t
ORIGIN      
        1 tatgcaaata atgtcatata agttacactt tagttatctt agttacaact ttgtcggttt
       61 actttttcta ctttcttcga tataattata atatattttt gatacaattc gacaaaaggg
      121 ggaatgctgt aattatggta aaaaaaagaa aaaagaaaac tgttacactt actatacttg
      181 ttgtaatagt tattggtatt ttttctatgg catgggcgga catgtctaga aaaaaagaaa
      241 tgaaagaaac tcaagaaaga cttaaagctg aaagattaga aaaagaaaga attgaaaaag
      301 aaaaaaatga acctattatt ggtgcaaaaa aagaagctgt aaaagaatat ggatatgacg
      361 caaaaatagt ttgggacaaa cttaacaaat acgactattc aaacaatgga gaaaagatag
      421 tctttcttac atttgatgat ggaccatcta ctactaatac tccacaagtt ctagatatct
      481 tgaaaagaca tggtgttaga ggaactttct ttattaaggg tgattctctt gaaagaaaag
      541 gagctaatga aattttaaaa agaacatttg acgaaggaaa tgcaatagcc catcactctt
      601 atactcatga ctataaaaaa ctatatccta acagaagcct aaatctcgat acattcgtaa
      661 atgaacttaa caaaactgat gaagctatga aaaaagtctt aggaaagaat ttttcctcaa
      721 atgtagttag atgcccaggc gggtacatgt catggaaaaa tatggaacct ctaggaaact
      781 acttaaaaga gaaaaacatg gcatcaattg attggaacgc tttaaatgct gatgcagaag
      841 gtaaaaagaa aaatgcacaa gaattatttg aacatgcaaa gaaatcttct gaaggcaaag
      901 aaatggttgt tcttttaatg catgatactt atggaaaaca agaaacagta aatgcattgg
      961 accaaattat aacatatttt aaagacaatg gttatcaatt taaaatttta atataataaa
     1021 aaagcaagtt tattgaatgt acattttata ctcaatagac ttgcttttac tttactctat
     1081 tttactagct cgatttttac aaattttttc tttcctattt gaacaactaa ttcactctct
     1141 tttacaatag tgcttaaatc ctctactttt tcaccattta cctttactcc accttgagaa
     1201 gctaatcttc taacttcact cttactctta acaatctcat tagaaactag tacttcgatt
     1261 aagtcaaatc catcttcctt aacttgaatt gtttgtatat cttcaggtat ttgacctttt
     1321 tggaatacag atttaaatct ttcttcagct tctttagcag attcttctcc atggtataat
     1381 gttactattt ctcttgctaa gttcatcttt atatctcttg gatttactga accctctttc
     1441 aactgagatt ctattttatt aacttcatca ggatgaacat ctgtcactaa attgtagtat
     1501 ttaattataa gttcatcagg aatttccata gatttttggt acattattcc cgcttcttca
     1561 tctattccaa tatagttacc taaactctta ctcatttttt ctttaccatc taatccttct
     1621 attaaaggca tcattattac tatttgagac tccataccaa attctctttg taatgatctt
     1681 cccattagta aattgaatct ttggtcagtt ccaccaagtt ctatatcagc ttctaatgct
     1741 atagagtcat atgcttgcat taatgggtag aagaactcat gtactgatat tggcatttgt
     1801 ccttcatatc tctttttaaa atcttctctt tctaacattc tcgcaacagt aatagttgct
     1861 gctaatttta ttacatcttc aaaatttaac ttagctaacc actcgctatt aaatcttaca
     1921 atagtttttt ctttatctaa aactttgaaa atttgttctt cataagtttt tgcatttgca
     1981 agaacttgct ctgttgttaa agcttttcta gctttagatt ttccagttgg gtctccaatt
     2041 tttcctgtaa aatctccaat tattattact atttgatgac ctaaatcttg taattgtttc
     2101 atctttctaa gaactactgt atgccctaaa tgtatatctg gagcacttgg gtctagaccc
     2161 aattttacta tcataggctt tccttctttt tctgatttaa taagcttttc ccttaactct
     2221 ttctcatcta ttaaatcatc tacacctttc ataattattc gcatttgctc gtctatcgat
     2281 ttcatttttc attcctccta aaatatttat ataatctata atttttattt ttataataaa
     2341 aaaagctttc atcctcttaa aaggacgaaa gcattgctta cgtgttacca ccttagttca
     2401 tcaatatctc acgatattaa tctcttagag tacaattaca tatactcgtg tactataacg
     2461 tgtactaatc cgatatctct taatggttgt tatctttcag agatagtgct ctgagatgta
     2521 ttcaaactaa atcaatttat tccatttcac caaactggaa ctctctgtta aataaaatct
     2581 agccctactt cttctcttca tagcatttat atattcagat tatttcatca attacaagaa
     2641 atttttctta acttactcat aatatattat tatttttata ttttgtcaac cttttttatt
     2701 attatataca tgttttttta cattatacta cttttttata ttttttacca atacccaaaa
     2761 tttatttttc aatcaaaaga tttatttgtt tattgactat attactctga ctcaatctta
     2821 tatttatttt cttatttaaa aatgcgttta taattattaa actaataact gttacatcaa
     2881 atataaaaaa cagcaataaa attattatca aaaataaaat tctagcatat aataattttc
     2941 taaacttagc tattataaag ttttgtagtg acatacatga gtctctaatg ataaaataga
     3001 cacttatata ggttattgat ataaagattg tttttaaata aacagaatca actttaacat
     3061 tcaacagttt tagaacttca aaaatggcta gataagaaac acatatgtaa aaagcttttt
     3121 ttgagcaaca taaaaattgg tttaaatttc taaagttctt aaatattaaa aattttttta
     3181 aagaattaga atctaaaata tgcaatttat tccctaaaat taaacttaat aaatatatgc
     3241 aaaacaccat accaaaagga aacaatgcta tcattaaata ataaataaaa tttgtatacc
     3301 cattaaaata aatccatttg ctatatcctt gaaattcttt cataaaacta taacctatta
     3361 atgttgaagc atatatatca atgaaaacca ttacaataac cccaaatact gaaccatata
     3421 ctaaatcatc cataaccatt gtaggcttat ttattagata gccacacatt ataccaagta
     3481 atacactctg acttgcccaa atagcagttc ctagatttcc aagtaccaga gaaattatta
     3541 aaagcgaagt aattccagat aaaatggtat actttaaatc acatttcaag cagattatac
     3601 aaaaaaatac tggcactata aaatctaagt aaatagcata tccaaatcca gtactcacag
     3661 ctataattgt tactactatg aataaagatg acaataaagc tgcttcagtt aattttttag
     3721 tagtattcaa aacactgcct ccataaatca taaattttct aactaaagtt ttgtttttaa
     3781 tatagtatat cactttttaa aaatattttt aataaatata aattttaata aaggtattta
     3841 tgttaaaaat ggtataatgt atcttaatat tcagtttttt aagatgctag gaggtaatta
     3901 ctatgtttaa aattaaacac tttaatgatt tgagtttaga tgaattttat gaaatagcaa
     3961 aatcaagata tgaggtattt gcttgtgaac agaagatatt ttcattaaac gattatgatg
     4021 atatagataa aagctcttat cacatatttt taaaagaaaa tggattgatt tgtgcttacg
     4081 ctagaataat tcctaaagaa tacagctctt ataatgatgt ttccattggt agagttttag
     4141 ttctttctag tcatagaaga aaaggattag caaaacaaat gatggattgt gctatagatt
     4201 ttataaaagt taacttacac gaaaataata taaccttatc agcacaaact tatataaaga
     4261 acctttactt atcttgtggt tttaaagaaa tctcagaagt atacgacgaa gcaggtattg
     4321 aacatattaa aatgagatta tagtgttgac aagctaatat tgttaatata taatttaagt
     4381 ataaattaaa tattgaattg atatttaggt atttgaaaat accttacata gagtcagaag
     4441 gcttttgctt tctgactttt ttattatcaa aattcaataa ttatatggat tcaaagtgtt
     4501 aatttttatt ctatattcta ctttccccaa aagtaagaat tatacccaac tttgtatgat
     4561 aggcatcttg cctatctttt ttttgtaaaa gtaacaaggt aataatataa tgcatagttt
     4621 aaaataagaa gattagccca ggctaaattt caagcatgga ggtgctatta tgaaaaacct
     4681 taacgatatt caagtgaaaa aaactgtcaa agttgaagat atattatcta gtggtaattt
     4741 aagagaaaga atgttagcgc ttggtcttac tagaggagct gttatagatg tagttagaaa
     4801 aggtccaaaa aataatttaa ctgtctataa aatcagaggt agcaagatag ctctacgaca
     4861 agaagaaagt agcttaatat tagtttctga catttaaaaa tttctagcag gaggatatta
     4921 atatggggtt gacacataac tcaactaaaa tgagttcttt aaaagacatg tttgatatag
     4981 acaataagga agaccagttt gtaattgcat tggctggcaa tccaaataca ggtaaaagta
     5041 ctgtatttaa tcatcttaca ggacttagac agcatactgg aaactggcca ggaaaaactg
     5101 tagctacagc tcgtggtaat tttaaatata aaaataccga atatgcatta atagatttac
     5161 caggaacata ttctttattt gccttatcac aagaagaaat tgtagctcgt gattttatat
     5221 gttttggtaa tccagatgca gtaatagtag tatgtgatgc tacatgtctt gaaagaaatt
     5281 taaatttagt atttcaagtg atggaactta cagataaagt tattttgtgt attaatttaa
     5341 tagatgaagc tagaaaaaaa ggcattacta ttgataaaaa acttttagag gattctcttg
     5401 gtattccagt tattttaact gctgcaagaa atggctctgg tatggatgaa ctattggata
     5461 ctttaaatga tgtatctttt gacaaatata aattaaataa taaacctgtt agatacaatg
     5521 aaaatataga gaatgtggta aaatcaattc aaccagaatt ggataatatt ataccaggta
     5581 taaattctag atggttggga cttagactta ttgatggcga tgaaagtata tttgaatcta
     5641 tgagtaatta tatagacaaa gattctatag atgctattaa tgaagtaaag aaaaagattc
     5701 cagacaatat aaataaacag aaaataagag atgagtttac taaaatcaac tatgattatg
     5761 ctaaaaaact gagtgatgag tgttgttcta atgttgctaa aaaatcaact gatagagaag
     5821 aaaaagtaga taaaatatta acatctaaaa tatttgggct tccaataatg ctcttattat
     5881 taggtactat attgtggatt actatagaag gagctaacta tccatccact ttattatcta
     5941 atttattact cggatttgag ccaagtattt cgggtatatt aaatagtatt aattgtccat
     6001 catggttaaa tgatatgttg gttttaggat tatacagaac attagcatgg gtaatctctg
     6061 taatgctacc tcctatggca atattttttc cattatttac cctacttgag gattttggat
     6121 atttgccaag agttgcattt aacttagacc acctatttaa aaaagcttgt gcacatggaa
     6181 aacagtgttt gactatgtgt atgggatttg gatgtaatgc agctggagtt attggttgta
     6241 ggataattga ctctccaagg gaaagactta tagctatact tacaaacaac tttgttcctt
     6301 gtaatggtcg ttttcctact ttgatagcta tatctacaat attttttagc tcagttataa
     6361 ccaactcttt cgtatcaagc gtagcaactg cactttgcat aactttactg ataattctag
     6421 gtgttataat aacattgttg gtttcttata ctctttctaa aacgctatta aaaggagttc
     6481 cgtctacatt tacattagaa cttcctcctt atagagttcc tcaaatagga agaactttat
     6541 atacctctat aatagataga acaatatttg ttttaggaag ggctgtaatg gtagccatac
     6601 ctgctggtgt cataacttgg atatttgcaa atatatatat aggagattta agtatattat
     6661 cacatgttgc aaacttttta gaccctttag ctaaattaat tggtcttgat ggttttatat
     6721 tacttgcttt tattcttgga ttcccagcaa atgagatagt tgtacctata cttttaatgg
     6781 cttacctagc aactggttct atgatagaat tagatagctt tagtgcttta ggtcaagttc
     6841 ttagagaaca tggatggact tatttgacag ctttaaatgt tatgctgttc agtttactgc
     6901 attggccttg tgcaacaaca cttttaacaa taaaaaaaga aactggtagt ttaaaatgga
     6961 ctgccttagg attcttaatg cctactatac ttgcttttgt tgtttgtttt ttaacaacta
     7021 cagtttacaa tttatttata taattagtat taactgacag tagacatata ttagagaaac
     7081 catttaaaaa tttatatact ttattataaa gcatagagat atattcaatg ataagtaaaa
     7141 ttacttaaca tttgaatata tctctatttt aattcttatc tggatacttg gattgaaatt
     7201 tcagtcatat aattatcttc attactttca caaaaaaagt caagtatact ctcctcatac
     7261 gaatttccaa caattttata ttcattttca tatatatatt ttttcaattt ttcgtaagat
     7321 ttatatgaat cctcatattt tccaatatgt tttatgcaag catacattcc ttctggcttt
     7381 acatatagct ttttatcctt tattttttta tctatcttca aataaaaact tgatatactt
     7441 aaatagtccc cattttttat attttcatta cttactatta ctcccaactc atatccttga
     7501 tacaaatttt tcgatttaca ataattggta aaatctatta attccatcat aaaatcacta
     7561 ttactcatat tactcaaatc gcttgataga actaaatatt cttcaaactt tttggatata
     7621 taaatttctt cactattcat tttagaaatt ccttcaactg ttatatttat tctattttgc
     7681 atcttagaac ttatctttct tagttttttt attttttctc ttatcttttt ttcttcttct
     7741 ttaagcattt tcacacaaag ctggggagtt ctattttgta tatactcttt tatttcctct
     7801 aaactcattc ctatttctct taatgattgt aatatatcaa gtgtatcata ttgataaact
     7861 gagtaatatc tataattatt tttatcttta tattcaggag aaaaaatacc tattttgtca
     7921 tagaaaatta atgtctgctt agatatacca cacaatttag caaattcacc tgttgtaaaa
     7981 tactgttttt ccaagggtta tacactcctt ttctttttgt tgtctatata gttactatat
     8041 tctttattat ttactaaatc aagtagttat tattttagaa aatcaatatg gttttgattt
     8101 taaagcttaa atatcagtta gatgtcagtt ataaatattt tacccattcc catcataaaa
     8161 aagatatccc ctcttaactt ttggatggaa tattatattt atacttgtta gagtgaaaat
     8221 tgtaataggt gctacaataa gatatttaat taaacataaa gttaattatt gtaataaaaa
     8281 atctttagat aaattagagt taaagtatgg aaatatagat agagaaaaag ctgttaaact
     8341 agaaatat
//
LOCUS       DAMKMI010000155         8322 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMKMI010000155 DAMKMI010000000
VERSION     DAMKMI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.161"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..1577)
                     /locus_tag="PC429_003247"
     CDS             complement(<1..1577)
                     /locus_tag="PC429_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3848723.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKV"
     gene            complement(1567..2415)
                     /locus_tag="PC429_003248"
     CDS             complement(1567..2415)
                     /locus_tag="PC429_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3848724.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            complement(2645..3697)
                     /locus_tag="PC429_003249"
     CDS             complement(2645..3697)
                     /locus_tag="PC429_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3848725.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            complement(3697..4371)
                     /locus_tag="PC429_003250"
     CDS             complement(3697..4371)
                     /locus_tag="PC429_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848726.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            complement(4476..5312)
                     /locus_tag="PC429_003251"
     CDS             complement(4476..5312)
                     /locus_tag="PC429_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848727.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            complement(5314..6357)
                     /locus_tag="PC429_003252"
     CDS             complement(5314..6357)
                     /locus_tag="PC429_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848728.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            complement(6376..6915)
                     /locus_tag="PC429_003253"
     CDS             complement(6376..6915)
                     /locus_tag="PC429_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848729.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            complement(7351..8040)
                     /locus_tag="PC429_003254"
     CDS             complement(7351..8040)
                     /locus_tag="PC429_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848730.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
BASE COUNT         2770 a         1446 c          805 g         3301 t
ORIGIN      
        1 aaaactttac taatattgga tatggattta aatctagtat cacttttagt tatttcatct
       61 aagtccttat catagttgac tataatttca tctccattat ttcttatttg tatttcatca
      121 acttttaccc aattattaaa atctcttaaa ttcataaatt ctatattctg tttttctaaa
      181 tattctattg tgttttttag atactctatg tttaaatatg aatgaaagaa gaaccctcca
      241 ctaaaacctc ttacaatgga taatttattt aaattttctt ttatattttg aaaagtaaac
      301 ttatcttctg ggtctatata tcctaaattt tcgggtataa atatattaaa ctcctctgta
      361 tccctaataa tataaggata tgtatttgtg ctaaattttt tatcattatt ttgatgttgc
      421 cccatataag tagaaaaata tttttttaat tctttatatc cctctgattc cattgcataa
      481 tgtggcgctt caaatgcaag aggataaatt ccattctcta tacatactct aagaccattc
      541 aaaactctat ctttgacaaa tatcttcata ttttctttta agggttcatc ttttttacca
      601 tcccaaaatt catacccttc tccagaaact tcttctttct tgtattgatg agtatatcca
      661 tgcaatatca cagttccacc tttatcttgc atatatttta ttgctttgac aatttcaggg
      721 ctttctgata aagttattac cttatgattt ttaggattta catatgctgg tattaatgca
      781 atcgtaaatg gtatattctt actacttaaa taatccgcta tctctacaag attttttggt
      841 tctctaaaag catgtacatc ttctattctt acaaatatac gtcctttatc aaacgtctta
      901 atattaaaaa tatcattaag tgaatcgcaa aatatataaa ataaaactcc atctaaatct
      961 aattttgata cataaaaaag atttttatca tttattatat atggatactt atttaaggta
     1021 tcattaatac ttcctattac tttattactt gtatcttttg attctacaat gttaaaaaca
     1081 taatgttcat ccaagttata tgatttacct ctataattta cactaactat attgtttgtt
     1141 tttcctacat agtctaaatt gtatttttta tgttccaaaa gattttctat accatatcct
     1201 aaccaacata tagttccttt atatgaattt aaggaactaa tcaagttttt agtgttttta
     1261 tcattattat atgagttttc attgattcct aaaataaata tatgactgta ataattttta
     1321 tttatctcac catcatagtt actcaatttt aatagctcta tatctgaact aaatcttccc
     1381 aatagagtcc tcataatatt taaaatatct ctattataag ctgtttcttt cttagaatca
     1441 tatatgatta ataccttatc tgtgctggaa gtaatttctg atgcatatga agataagcta
     1501 aaatttgata ttgtcagtac tattgataat acaactacta attttaatag acttttatta
     1561 aatattttaa acatcatatt gaagttcctc ttcaactata ttcttaaaat ttatactatc
     1621 tccaaaacta ctcttatatt gtaaaaatgc aattttgaca tctatattta catattttcc
     1681 cctattgttt aaatctaaat ttaattcttt tatccttttc tttattctgt ctttaacaac
     1741 ttttgaacct tccaaagatg tatttggcat tagtatccct atcatatctt tttgtaaact
     1801 ataaattata tcttcatttc ttgtacattc tataatattt gaaccaatat attttactat
     1861 tttattagtt ttgttttcac caaatatagt ctgtaggttt ccgtagtatg gtaattttac
     1921 tatcataaga gaaaaatcag tattatgtct aatagattta cttatctcca tatttacatc
     1981 attataaaaa atctttaaat ttctaagtcc agtttcacta tcaatagtca ctaattcctt
     2041 atactgttca gatagttttt tatttgtatt ttgcaattca tttatgcttt tgttcagatt
     2101 acccattata attgaagata ctggtgttgc tattatccac atatatgata taaattcaac
     2161 atccatattg tgagtaatat tgttatataa aatataggat gtatacataa atataattat
     2221 tgaacttgtt atcaacccca ctactgaatt tgataaataa gttaacatta ttataaaaaa
     2281 aattattgct aacattatga acacattcaa tttattttct gtcctaatcg atagtattac
     2341 tattccaaca attaaaaata taaaaacgag caaagccaat attaataaat ctattttttt
     2401 atttatccta ttcatttttt ataaatttac acctccaatt attctattaa gttgcccatt
     2461 ttttctaatt attattaaag tcattataca aataagtttt catttattaa taaactattt
     2521 aaattgcata tgaatataac ttaaacaaaa aataaaactg ttcaataatt agaaaataaa
     2581 ttttctaaaa aagaacagtt ttattaagta tttattaagt aaatttactt aaaacaacaa
     2641 gaccctattt tgattttaac tctatccaag ccttgtcata taattttata ttttcaccaa
     2701 tatcttcaaa agtttcacat ttatcaagta cttctttaga tggatatgca acaggattct
     2761 gagttatttc tgggtctaat aattttagtg ctcctgtatt aggtgttgaa tacccaatgt
     2821 actcagcatt tacttttgca ttctctgggt ctaataaaaa gtttataaac agctcagctt
     2881 ctttcttatt ttgtgctgtt gtaggtacac acatagcatc tacccattta ttagttccct
     2941 ctttaggtat agcataatca aggtcaggat tttgctccat tagtgtaact gcatctccag
     3001 aatacagcat acccattgcc acttctccac caagcaatct atccttacct tcatcattta
     3061 cataagccaa tactaaatcc ttttgtttca ttaaaagatt tttagcttca tttatttctt
     3121 ttggatttac actattcatg ctatatccta attttttaag agttatccct atagtatctc
     3181 taacagagtc aaacatcatt atatttccct tatactttgg attccaaagt atattccagc
     3241 tatccatagg gtctttaact tctttcttat tgtacaatat tccaagagtt ccccacatat
     3301 atgcaacact atattcatta gttttgtcgt atacaggatt taaaaaactc ttatctaaat
     3361 atttcatatt aggtatattc ttaaaatcta atttttccaa aagaccttca tttttcattt
     3421 tctctaccat atagtctgaa ggaaatacta aatcatagtg tgtactacca ctctttactt
     3481 tttgatacat catttcatta gtatcataag ttgaatattg aacatctatt cctgtttctt
     3541 tttcaaactt atctataaga gactcatcta tataatctcc aacattatat acatttaaaa
     3601 cttttgtaga atcagttgct ttattacatc ctgtaaataa agtaactgtc atcataccta
     3661 cagctataag ggatattgct tttttaaact taatcattat ttaatccccc ttactataga
     3721 ttctttttta ttagctagta aaagtaaacc tagaaccact gcaaacatta ttgttgataa
     3781 tgcatttatt tcaggtttta tacctcttct agccattcca tagatttcta tagaaagatt
     3841 gcttacacca tttccagcat taaagaaact tattataaag tcatctattg acatagtaaa
     3901 tgcaattaaa aatccagaaa ctatacctgg ttttatttgt ggtaaaatta cttttctaag
     3961 agcgtatata ggtgttgctc ccaagtccat agctgcatct tctatattat caggtaattg
     4021 cttcattttt ggcaaaactg aaagtataac gtatggcaaa caaaacatta tatgtgccaa
     4081 tagcattgtt gtaaatccaa attccatttt tacaaataca aacagactca taagagctac
     4141 tgctgtaact atctctgtat ttaaaatagg aagatagttt acatttagta ttaatttctt
     4201 actcttacct ctcatcttat gtattcctat agcacttata gttcctacta ttgtagatat
     4261 tacagacgct aatatagcta ttacaatagt ataatataaa gcactcatta tactctcatt
     4321 ttgaagtaat tgaccatacc acttccatgt aaatccattc catcttgcca ttgattttga
     4381 atcattgaaa gaaaatagta ccaaagcaaa tatgggtgca tacaaaaata agaatactaa
     4441 tgctaaatat atattagaag ttattttctt taattttaaa atagtaaccc accgccttct
     4501 ttgtcagatt catctccaaa ttttgacatt atagacatag atattaatat tactatcatc
     4561 ataaatatcg atatagctga accaaagttc caatctccaa caactgtaaa ctgttgttca
     4621 atcaaatctc caaccagcat tattttacct ccacctaaca gtcttgatat tgcaaaagta
     4681 gtgactgctg gcataaatac catagttatt ccagacatta cacctggcaa actcaaagga
     4741 aatataattt ttctaaaaac tgtcatatta tttgcaccca agtcatgagc agcgtttatc
     4801 aaatcattat ccatctttga tagtgctgta tatattggaa gaaccataaa tggtaaaaag
     4861 ttatacacca tacctaaaag tatagcaaaa ttagtataca aaattgctaa tggttgtatt
     4921 ccaaaccaac ctaaaaatgt atttaaaagt ccattttttc ctaatatagc aacccatgca
     4981 tatgttctta aaagaaagtt catccacata ggaagaataa acaataatat taatgaacct
     5041 cttctactta cacgagcctt tgaaattata tatgccactg gataaccaac tataaggcat
     5101 attaatgtag ctcctcctgc taaaagaata gaacgcccaa aaactttcat atatataggg
     5161 tcaattactt ctttataatt ctcaagtgta aaggcataac ctccacctga ttcttttgta
     5221 aaactaaaaa atactactaa tattaaagga attattacaa atatcgcact ccaaactaca
     5281 tatggatatg ctaaaaaaga ttttcttttc atcttagtta ttctcttttc tcattatatg
     5341 gatatcttct ggatatatat ccatgcctag ttcagtccca acctcagcat ttttagtatt
     5401 atgaagtatc cacaatcttc cattttcatt caattctatc tcgtaatgca ctcctttaaa
     5461 cactgtagat gtaactttcc cctttaacat tccttcttct ggctttacca tttttatatc
     5521 ttctggtctt attactactt ctatattttc gtttttttcg aatcctttat caacacactc
     5581 aaaatctcta ttacagaaat taactttata atcttctatc ataattccat caaatatgtt
     5641 actttcacct ataaacttag ctacaaatga atttttaggt tcattgtata tatcttgtgg
     5701 tgttcccatt tgttgaattt tgcccttata cattacaaca atagtatctg acatagtaag
     5761 agcctcttct tggtcatgag ttacaaaaac aaatgttatt cctaattttt tttggattct
     5821 ctttaattct atttgcatct cttgtcttaa ctttaaatca agtgctccaa gtggttcatc
     5881 tagtaataat acctttggtt cattgactag agctcttgct attgcaattc tttgttgttg
     5941 tcctccactc aatgattcaa cttgtcgtct ttcaaaacct tctagtgcaa caagtttcaa
     6001 aacctctttt actttagcat ctattacatc ttttggagtc tttttaattt ttaatccaaa
     6061 agctacattt tcataaacat ccatgtgtgg aaataaagca tatttttgaa atactgtgtt
     6121 tacttgtctt tcatatggtg gcaaattatt tatctcctta ccttcaaata aaactttacc
     6181 atcatcagca tactcaaatc ctgctattat ttttaatgtt gtagtttttc cacatccact
     6241 tggtccaagc aatgttagaa actcattttt ctttatatct aaattgaaat tatctaaaac
     6301 agtaagttct tcataatgtt tagatatatc ccttaattct attatatttt ctaccaatta
     6361 ttaaacacct ctattctaaa atgatggtgg cgtacttacc caaatcactt ttgctgtagt
     6421 tttgccctcg tttgatatat agtgatttgc tttcggtcta aagtaaaaac tctctccttt
     6481 ttttacttta tttttctttt cgcctatatg gacatatatt gaacctgcta atacatatcc
     6541 aaattcttca ccttcatgtg gcttttcttc tttatactgt cctcctggat acaatgttat
     6601 tattattggc tccatttcat ttttttgtga atttggaatt aaccacttta atttatattt
     6661 taaatcttca tcttcagttt caaacatatc ttcttttgta aaactgattc tttcatcatc
     6721 tatttcatta aaaaattccc ttaaatttgt acctagtatt tcaagtatat ctataagggt
     6781 agctatggat ggtgatgtta agttattttc taattgagaa ataaatccct ttgacaattc
     6841 acatctatta gctaactctt cctgggtcag ttgtttttct gttcttaatc tttttatctt
     6901 ttcacctata tccatgttgc acctcctagt ttctgtttta ttttactaaa cattttgttt
     6961 actaaacatt gagtttaaaa tttctaaaca acataggtaa ttatatataa aatttggata
     7021 taatacaaga gtttttttga cattttttta tttttttatt tagaataaat acaagcattt
     7081 taaaggcttt ggaaaataca cagtttaaag gtgtttatat taatatctat aaagtttagt
     7141 atctctaatt ataaattaat tcattcatta aatagagaat aagacttcta gtactaaaaa
     7201 aatattgaac aaataaaaaa ttactagaaa tctatatttt atagtgttct agcaatcctc
     7261 atattttaat attcttttaa tattcaattt atctatctaa acaaaatgtt aatttaaaat
     7321 acttaacatt tcgttgaatt tattcttaaa ttacttaact atattttgaa cagcagcttt
     7381 tttctcacca ctcatataca ctttgataaa ttttatatca tctactataa atctatgttt
     7441 aatcttacta caatctaatt taaataagcc aaataccatt tgtgtgaaat accccaatcc
     7501 aaatgcactt attatagttc ctataccaac ttttccacct aataaaaatc ctactattaa
     7561 tgctccaact tctatagtac ctcttattaa tttaagtgat tttcctgttt tcttttgaat
     7621 tgcaaccatc aaaccatctc taggtccact tcctaaacca cttcccatat aaaataccat
     7681 tccaacagac aaaagaacca atcctaatat caacataatt attcctacaa ataaattatc
     7741 agcctctgga attaaattac tatataaaag taaatccata aaaaatccaa ttagtagcat
     7801 gttaaaaaca gttccccatc ctacattttc tccaagaacg acatcagcaa taactactat
     7861 agagcctaca attatagaag cagttcctat agttattcct atatgatttg aaagtccttg
     7921 atgaaataca tcccagggag ataatccaag gttacaattt atcgtcaata ctataccaac
     7981 tgaacatata aataaaccga ctactagctt taaaaatttc cataatactt tttgcaacaa
     8041 attctacccc tcctaaacat aatttaacta ctcactctaa atatcaaaag ccagcagttt
     8101 tcttacaaaa atcttatttt attaatttta taatatactt ctatgataca ttattttatg
     8161 ctaaataact accattttat tacaacttag ttacaatttt tgtcattatt ttttggtgaa
     8221 gtaatttata catttatcca accaatttct atccttcaaa tgttttcaat ttcatttata
     8281 ttatttataa atgaaaaaaa ctgtctaaat ataagttatt at
//
LOCUS       DAMKMI010000156         8277 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMKMI010000156 DAMKMI010000000
VERSION     DAMKMI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.162"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            102..1292
                     /locus_tag="PC429_003255"
     CDS             102..1292
                     /locus_tag="PC429_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3848731.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
     gene            1462..2424
                     /locus_tag="PC429_003256"
     CDS             1462..2424
                     /locus_tag="PC429_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF3848732.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(2546..3208)
                     /gene="zmp1"
                     /locus_tag="PC429_003257"
     CDS             complement(2546..3208)
                     /gene="zmp1"
                     /locus_tag="PC429_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF3848733.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYAEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(3308..3393)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3579..6497)
                     /locus_tag="PC429_003258"
     CDS             complement(3579..6497)
                     /locus_tag="PC429_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF3848734.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      complement(6615..6700)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(6827..8005)
                     /locus_tag="PC429_003259"
     CDS             complement(6827..8005)
                     /locus_tag="PC429_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF3848735.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
BASE COUNT         2592 a         1309 c         1014 g         3362 t
ORIGIN      
        1 tatattttca aacaagtaat ttttcgaaaa ttgtctaaat tcagtaaaaa tatggtaata
       61 tatacataag caatataaat ctataaaaag gagtgttaca tatgatatct aataaaatgc
      121 aaactttagt tgcaaatagt tctgttataa gagctatgtt tgaagaagga aagaaacttt
      181 ctgatattta tggtgaggaa aatgtttttg attttagtat aggaaatcca agtgttgaac
      241 ctccagagac tattaaagca gttataaatg atattttaaa tgaagaatct ccaaatttag
      301 ttcatggata tatgaacaac tcaggatatg aggatgtaag agatgctatt gctgaacaca
      361 taaataaaaa agatggatta aatcttacaa gagaaaattt aattatgact tgtggtgctg
      421 ctggtggtct aaatattatt ttaaaaactc ttttaaatcc tggtgatgaa gttattgctt
      481 ttgcaccgta ttttggtgag tataaaaatt atacagaaaa ctatgatgga aaattaatag
      541 aagttcctac aaatatagag acttttgagc cagatttaga tgctctaaaa aatgctatta
      601 ctccaaaaac aagagcttta ataataaaca ctccaaataa tcctactggt gtcatatatt
      661 ctgaagaatt attaaaaaat cttggagaat tattggattc aaaacaaaaa gaatttaata
      721 caagcatata cctaatttct gatgagccat atagagaaat aatttatgat ggtgccaaag
      781 ttccttgtgt actaaaatac tatagaaatt catttatagg atactcttat agtaaatcat
      841 tatctctacc tggtgaacgt ataggatata ttgttgctaa tgggcaaatg gatgattttg
      901 atgatgttat gtcttcatta aatgttgcaa atagaatact tggatttgta aatgctcctt
      961 ctctttttca aagggttatt gcaagaagtt tagatgcaga agttgatgta aatatttata
     1021 aaaagaattt tgaccttcta tataatagtt taatcgatat gggatattct tgtgttaaac
     1081 ctaatggtac attctatttg ttcccaaaag ctccaattga agatgataaa aaattctgca
     1141 atgatgcaaa acaatttaat ttattattag taccaggttc ttcttttggt tgtccaggtc
     1201 attttagagt atcatattgt gtatcttatg ataaagttaa aagttcttta ccagcatttg
     1261 aaaaattagc caaattatat aatttaaaat aactaaatca aagtaattca aataattaac
     1321 taccttagta tagaatactt acattctata ttaaggtagt ttataatttt atgtactata
     1381 ttttatacat accatattat tccaatatgc ttgaatattc ttatctatag tactaatata
     1441 aattacaagg aggtgtttga cttgaaaaaa ggttatgttt atataatact gactacctta
     1501 ttttttagtt caatggaaat tagtctaaaa actgtcacta atgactttaa tcccttacaa
     1561 ataactttat caagattttt tgttggcggt ttagtcttac taccttttgc tgtaaaaaga
     1621 ttaagagctc ttagtctttc tattacaaaa tcagacttaa aatactttgc atttttaggt
     1681 tttatgtgtg tagtagtaag tatgtcattg tatcaacttg ctgttctaaa cacaaaggca
     1741 tctgtagttg ctgttttgtt cagttgtaat ccagtctttg taatgctttt agcttactta
     1801 attttgaagg aaaaaatata taggcacaat gtaatttcac ttatattaga agttcttgga
     1861 attatagtta taataaatcc attgcataca aaactaactc ttagtggaat attcttaaca
     1921 ttatcttctg ctattatttt ttcaatgtat actgtatttg gtaaaagaaa aaccttaaaa
     1981 tttggtggaa tagttgtaac ctgctttagc tttatatttg gtagtttgga aatgttgata
     2041 ttggttttac tcactaaaat taattttatc gcagatatat taaataaaaa taagcttcat
     2101 gtatttgcaa atatccctat ttttagtgga tataccttac acaatatgcc cattatgatt
     2161 tatgtattcg tatttgttac aggtgttggt tatgcgcttt actttatggc aatggaagct
     2221 acatctacta gtcttacatc tttagtattc ttctttaaac cagtaatatc tccaatacta
     2281 gcattattaa tacttaaaga aattatccct ataaacatgg ttataggaat tttattgata
     2341 gtaattggtt caataatttc tattatacct acgataatta ctcaaatgca taataaacac
     2401 gttgaagcct tgtataagga gtagtaattg ataatctatc atagttatac ataagacaaa
     2461 atttattaga tttaaacgca atataaaaga gatgctatta taatagcatc tctttattta
     2521 tatttatttt aaatttatta aaactctatt tagctaaatt ttgcaaaaag ctataagttt
     2581 gtggacaagc tgattttaac ttactatttg tatcttgatt taaatagtaa tatgcaaatg
     2641 attctgcaaa aaattcttct gcatatacac ctaagtagtt tacattacct aaagaacgac
     2701 cttctttagc aaatatttgt ttgaattgtg ctgatttaga tatatcattt aatactatat
     2761 ggtctattgc atgtgctgtt tcatgtaatt ctaaatttat tgcatcatgc cctttacctt
     2821 tgttactaaa tcctattcta agagctactg tacttccacc taaacctgga acatcatccc
     2881 aagtttttcc agtaccttcc catcccttag gaactacacc ttttaaataa gcatattctt
     2941 tttcatctgt taactttcca cttaataatt ttatttttaa gttattttgc tttaatgcat
     3001 ttaaatactt tccatctata ttagctaatc tatttaccat agcattagct tcgtttttat
     3061 cgtaatttcc agttggaaaa actactattt gactaagtgt gtctttattt tgttgtatag
     3121 tagtactatc tgcatgtgca cttactggaa ctgaacttat taagaatatt gatattatag
     3181 ctattaataa ttttttactt ggtctcatat tattccctcc tttgagttag gccgggagta
     3241 ataaatttca aaataaaaaa gatacccaag agtatctttg tatatacaaa aagaaatttt
     3301 tactcttttc ggcaactggc tggcatggtt tactaaactt tagcccctat agctttgcgt
     3361 gactaggttt ccctagtttt gcctcattta attttatatc tatattatag cttatatctt
     3421 tttaattgtc tatatccctt tggaactttt ttctaaaaag ttattatttt tttacataaa
     3481 tcttcaaaaa aaaatatctt ctaacaatat tttcattatt aaatattgtt ttagaagata
     3541 ttttttaata taacttatat tttttataat gttagggtct aatttgtatt tttatttctt
     3601 cttaatacga taagtcctac aatagctcct ataaccaaaa gtatttctcc aataattgta
     3661 gtactatccc ctgtttttgg tggtactggt ggatttacca aagtatcatc tttaacttta
     3721 ttattttttt cttctgacac tgatggcttt actacttctg gtattggaat aatctgttta
     3781 acaggagttt ctatcatctc ttctgatggt ggtactggcg gattcaatac ctctggtatt
     3841 ggcggattta ttatatcttc tgatggtggt attggtggat ttataactat agtatcctct
     3901 gtatcagatg aacttgatgg tttccttggt ttatctgtgc ttggtggtac tggtggattt
     3961 actattggtt catctgtatt tggtggtgct ggtggattta ctggtggatt ttcttgtctt
     4021 atgttttcta tatttccaat atccactatt ctaccaatat ctttattacc aatttcaacc
     4081 ttaattttat ccttcagttt ttcatatcca cttggagcca ttatctccac taaataatat
     4141 gttcctggtt tttttatact agattgaatt ataccatctt catcactaac taaatcctta
     4201 actactatat ttccaaatgc atcttctaaa ttgaacttag cacctgaaag tacattttta
     4261 tctaaagcat ccactttttt agcttcaaaa gcataacctt gcttagtagt ctctactcct
     4321 ggaggacttg aaccattaat ctgagccaca ttttgtatag tctccccaac ttttacattt
     4381 gtgaagtctg catcaaatgt aacccaactt tgcacaggtt ccccttcttt tatctgttta
     4441 aattcgtatt tatttactaa tcttattgta ttttttccat ctccagcttc ttttttaagt
     4501 tcaagattat cagaatattt tatattagat atttctagag ctgagtttac ttcatctaaa
     4561 agatttactt gtcctgcatc ataaactcca taagttgaca tattgatttt gtatttaaca
     4621 atctggttat cactactatt taaaactttt ttatcaactt ctttttttgc gcttattaat
     4681 ccagaatttt tcttaaagtt tatctcatca tttttatcaa tttcactatt atttgcatca
     4741 agaacagctc tatttatata gctatcttca atactaatta cctttaatgt ataagctata
     4801 aaaaactttg attccacttc ttctttactg agattaattg taaatccagt tataacttta
     4861 tctacttgcc cactttcatt tcttttttct tctactttat atgaaatatt attggcaatt
     4921 ttttctttac tccaaggagt ccagctccca ttcattggaa tttctacaac tctaaaatca
     4981 tgcccagctt ctcctgaagt agttaatctc atgccttctg gtatattatc tgttataaca
     5041 acattactta ttggtgattt atcatcattt attgaaatct gatagtttat tacttgacca
     5101 acttgagtaa tatttgttgc tcctgaatgt cctttagtaa gaagataatc atctcctcta
     5161 tcaaatatac ttacagtagc atcatcttct tgtatcttct taccatcaga ttcaatccta
     5221 gctacattct tatatttatt accatgtttt gcgtttttgt cactgattct agccttatac
     5281 ttaacagtat acgatttatc tatatttcca aaattaactg aaatagaatt tttactctgt
     5341 tcaatattaa aatcactaac ttcttttcca tcacttgtta ctacacttac agaaccattt
     5401 atcaatgtca atccttcagg aagctcatca tacaaaataa tatcttttaa ctcttcctgc
     5461 ttttcattta cttgtatact gtaatttata tctttatttt taactggcat aaatacagta
     5521 gttgattttt ttccttcaac aagcttttta agtgttcctt tagcatctgg aacatccgtt
     5581 ttatctccag gttttatttc tacatctata tctccacttc cactttctat ttttccagta
     5641 gtttcaatat gtatttttgc attagtatct tcatcaacct ttttaacata tgcttccaaa
     5701 cctattttac cttgcattac actttggcca ttaggtaatt ctctaaatgt aagtgtatac
     5761 tctccattac tttctgtttt atcaaagcat tcacttgtat aatctaaatt aactttattg
     5821 aatacatctg gcaccttaaa agttattgaa tctcctgctt gtattccttt tgatgttgga
     5881 gtataagtta aattaaatga tatttttttc attgattccg tttctattgt attgttatta
     5941 atttttacaa caaatccata gtcttcattt tcttcattaa gttcttcttc taaaactttc
     6001 aataaatttt catcttctaa tagattatct tcctctggag ttttttctgt atttttattg
     6061 tctagtggtt tttcatcttc taaagcttgt tctgactttt tattatctgt tgacttatct
     6121 tcttctggta tctgctctgg ctttttatta tctattggct tttctccttc tggagtttgc
     6181 tctggctttt tattatctgt tgacttatct tcttctgggg tctgttctgg ttttttatta
     6241 tctgttggtt tttcttcctc tggggtctgt tctggttttt tattatctgt tgccttatct
     6301 ttctctggag tctgttctgg ctttttattg tcgctttcct tctcctctcc agttgatgaa
     6361 ttagttgaac cactttgaat ctgactattc tctcctaatt ctgaagcata agatacagtt
     6421 ggatatattg tagacaccac catacttaaa atcataatta aactaattaa taatgccttt
     6481 ctatttcctt tcttcatgta aatctcaccc ctttaggtta cctataattt ataatctaat
     6541 cttccattaa tatcgtatat aaagtaattt ttactaaaaa attatacaaa aaaagctatg
     6601 aaaataaata aatttatatt catagccggt tgcaccactg actcagtata tccataaggt
     6661 gcccaataat agaatatact tcaacatctc tataatattt cataaaaaca attaaaattg
     6721 ctttatttat attaaaaata caattatata cttctattat attatgcctc tatactattg
     6781 tctatccttt ttacattttt taccttatga tgagattaat taataattaa ataaaatcct
     6841 tatctacaac aattacacaa ttatattcag gatttatctc cttaactaat tttgttatat
     6901 cttcttttaa gtcatcttct gacatgtttt tggaaaattc ggaaggatta actactatat
     6961 caaatattaa attcttcttg cttccttttc caactattct aaaatcatgc atagatttta
     7021 caagactatt tttcttaagt atttcttcaa gttctttttt ggctgacata acttctttat
     7081 cttcaagaca tattgggtcc atgtgaatta ctagatatat atctaatttt tcagatattt
     7141 ctctctctgc agtatctata atttcatgta tttccattat atctatgttg gatggaattt
     7201 ctgcatgtat agatgcaata catctaccaa ctccatagtt atgaataatt aaatcatgtg
     7261 tacctattat atatttataa gataacaata gctctataat agaatttacc atttcttcat
     7321 ctggtgcttc ccctaaaaga ggatttatag tatcttttac aagagagaaa ccagcataaa
     7381 gtatcactaa tgatactacg ataccaacat atccatctat aggaaaatta gtaaatctag
     7441 ctacaataaa tgatataact acacagcttg aagtaaatac atctcctaaa gcatcaagtg
     7501 aaactgcttt taaggctgaa gaatcaattt tatttcccat aaatttatta aaacgactca
     7561 accagatttt aatcattata gatgcaatta aaagtataag tggtattact tcaaatttta
     7621 caggaattgg atttactatc ctttctatag atgactttac aaattgaagt ccaaccaaca
     7681 tcaccataaa tgctactatt aatgctgata aatactctat tcttccatga ccaaaagggt
     7741 gttctttatc agctggctta cttgctaatt taaaacctat catagttatt gctgaagatg
     7801 ccatatcaga aagattatta aatgcatcag ccattatagc tatactagat gtgagcattc
     7861 ctatgaatac ctttatcaca aataaaagta aattggataa tatacctact acaccagcta
     7921 cataaccata cttatttcta acatctgtat tttgaacgtt ttcgctatct ctgataaatg
     7981 ttttgactag gattttggaa aacatgaaac ttacctccct gccctttatt ttattaacaa
     8041 attttataat ctatatcttt atttatgata acataattta tatataaaat tcttaaaaat
     8101 cactctactt cagtaaaata ttattctata tatctattta gatacgaatt ttatatttag
     8161 taaagttttt atggcttagt gtctgtttaa tagattctat ataagttttt acaaagatta
     8221 tttattatga taatatcatt tttcccaagt attacttaga atatgatttt tttgttt
//
LOCUS       DAMKMI010000157         8167 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMKMI010000157 DAMKMI010000000
VERSION     DAMKMI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.163"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..597
                     /locus_tag="PC429_003260"
     CDS             <1..597
                     /locus_tag="PC429_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3848736.1"
                     /translation="GLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQS
                     LSLIMSCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALH
                     YGDRLIMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            728..1750
                     /locus_tag="PC429_003261"
     CDS             728..1750
                     /locus_tag="PC429_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3848737.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(1914..2471)
                     /locus_tag="PC429_003262"
     CDS             complement(1914..2471)
                     /locus_tag="PC429_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF3848738.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPIKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2477..3553)
                     /locus_tag="PC429_003263"
     CDS             complement(2477..3553)
                     /locus_tag="PC429_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF3848739.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC429_003264"
     CDS             complement(3554..4237)
                     /gene="modB"
                     /locus_tag="PC429_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF3848740.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC429_003265"
     CDS             complement(4386..5198)
                     /gene="modA"
                     /locus_tag="PC429_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3848741.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5391..6320
                     /locus_tag="PC429_003266"
     CDS             5391..6320
                     /locus_tag="PC429_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848742.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6481..6930)
                     /locus_tag="PC429_003267"
     CDS             complement(6481..6930)
                     /locus_tag="PC429_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848743.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENKKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7133..8113)
                     /locus_tag="PC429_003268"
     CDS             complement(7133..8113)
                     /locus_tag="PC429_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3848744.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2825 a         1349 c          979 g         3014 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaagta ttattaaata aacataactt gtctaattta
       61 gctgaacaca gaagaactca aatagtaagt agagtttttc aagacccaag tcttggaact
      121 tgcccttcta tgactgttag agaaaacttg tctttagctc ttaacaaagg aaaactatta
      181 aatctaagaa aatgtcttcg ttacaaaaga gatttcttag aaaatttatt ggaaggtgta
      241 tctcttgatt taaaaaaata ccttgatgtt caagtaaaat ttttatcagg aggtcaaaga
      301 caatcattat cactaattat gtcgtgctta acaaatccat cagttttatt acttgatgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa ttgaattgac taataagatt
      421 gtaagggaaa aaaatataac tacacttatg gttactcaca acttaaaaca cgctcttcat
      481 tatggtgata gattgataat gcttcataag ggtgaagttg tattagatgt aaaaggtaaa
      541 gaaaaagaac aattaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtttaaaat
      601 aatatatcta ttgtattaaa ctcggcttta aaataaagtt aaaattacaa taattatggt
      661 tactttgtaa taattcatat gaatatatga acatatataa caataaattt ttgaggggga
      721 tataaaaatg attaataaaa aaagattggc aagtttgatt ttagctggtg ctcttagtat
      781 atctatgctt acagggtgtt cacaaggagg agactctggt aattcaaaac aagaaagtaa
      841 ttctaaaggt aaagaagtta aaaaaattgg tataactcaa ctggttgaac atccagcatt
      901 agatgccact aggacaggtt ttgtaaaagc attagaaaag aacggattta aagatggaga
      961 aaatattgat atagacttcc aaaatgctca aaatgacatg cctactacac aaagtattgc
     1021 gagtaaattt gcatctgata agaaggactt aatatttgct atatctactc catcagctca
     1081 ggcagcattt aatgctacta aagatatacc tatacttatt actgctgtga gtgaccctgt
     1141 tgctgctgga ttagttaaaa ctcttgaaaa gccaggcaca aatgtatctg gaacatcaga
     1201 ttttgtttct gtagacaaag gtttagaact tcttaaaata tttgctccta aggcaaaaac
     1261 tataggagtt atgtacaata ctagtgaagt aaattccaaa gttcaagttg atgctttaaa
     1321 agaatatgca tctaaaaatg gatttaaagt tgttgaaaaa ggtataacta cttcaaatga
     1381 agttaaccaa ggtatttcta gtttagttgg caaaatagac gtattatatg tccctacaga
     1441 taacttagtg gcttcttcta tgcctatagt ctctaaaata gctactgaga ataaaatacc
     1501 tgttatagca gctgaatctg gtccagttga aaaaggtgct cttgcttgtc aaggtatcaa
     1561 ctacgaaaaa ctaggttaca aaacaggtga aatggcagtt aaaattttaa atggcgaatc
     1621 agtatctgat atgcctgtgg ctacatcaga tgatacagat atcattgtta atgaagatat
     1681 attaaaagct ttaggtatgg aaaaaccttc taatgaaaat atttcttatg taaagactaa
     1741 acaagaatag aaagatattt tctatatacg attaatatgc ctgtttctat aaattaattt
     1801 atagaaacag gcatatattt ttattttaat gtattatcta tttaaagatt atatgtaaat
     1861 gctcttaatt tgtttgtata aattttactt gtataaactt tatacaatca tatttattct
     1921 tctattttct ttattactct acatggattt ccaacagcta cagtatttgg tggtatatct
     1981 tttgtgacta cacttcctgc acctattact acattatcac ctatagtaat tcctggtgtt
     2041 atgattactc ccccacctat ccatacatta tcacctattt ttataggaga accatactct
     2101 atgcctgaat ttctaagttg tgcatcaatt ggatgatagg ctgtatagat ttgaacgttt
     2161 ggagctaaca ttacattgtc acctatttca attttacata catctaaaaa aatacaatca
     2221 taatttgcaa agaaattttc gcctacatgt atgttatatc cataatcaca cctaatattt
     2281 tgttctacat ttatttgctt gccaactgaa ccaaatagtt gtcttaaaat atcttctctt
     2341 ttttcatatt catcctctaa agtattatta aacaatctag ttaatttttt acaatactct
     2401 ctttctttta ctaacaattc atcatttgca tagtatcctt tcccagacaa cattttttct
     2461 ttttcagtca tatagtttac tcctttttgt tataattgta tctaaagcaa aataaatatt
     2521 cctgtggaaa atctaaatat attctgtcac ctttcttaaa tctcatttta cttttttcca
     2581 attctatttg aataggtaca cattcgttag ataaatcagt ttttctaaca tatatagtat
     2641 atgtaaaagg attttctaca atgttaatca attcgaaaca taacttttca tttaaatctt
     2701 cattagaatc caagacctta atatgatgct ccctgattcc cacatattct atattatcac
     2761 ctatttctct atttaatgtc aactcgcatc cccagtctgt agcataaata gtatttttat
     2821 ccagtctatt taatttggat atatttttac accctgttat cttagcttct ataaggtact
     2881 ttggagattc aaagagctct tttttaggtc tctttggaag accttctcct ttgttgtaca
     2941 ctataatatc atcacaaact cggtaagctt cttcaatatc atgagtaaca aatagtatat
     3001 ctcccttgta atcttttaac atattcataa gttctttttc catattgctt cttaaatgat
     3061 ggtccaatgc tgagaaaggt tcatctaaaa gtaatatatc tggagatgtt gccaatgctc
     3121 tagctagtgc taccctttgt tgttgaccac cagataattg ccaagggtat ctcccctcaa
     3181 acccatttaa tttaagtata tctaagtacc ttgctactat ttcctttttt tcactcttac
     3241 ttagttttaa aagcccaagt tctatatttt gacttacagt catatgtgga aacaaagcat
     3301 agttttgaaa taaaaatcct acctttctat cttgtgttga taaatttatc ttcttttcag
     3361 aatcaaatag aactctatca tttaatacaa ttttacctct tgttggtttc tcaagccctg
     3421 ctatacattt taatgtcata ctttttccag aaccagattc tcccaaaaac cccagagtac
     3481 ctttttcttg tttaatttct acctttagtt taaaagagga taaatctttt tcaatatcca
     3541 cgtataagct caactatcca cacctctttc ctataatttt ctgctgaaat tccgaccaat
     3601 aatttagtaa taaaatcatt gctatagata ttgctacaat aatcataacc cacatcatag
     3661 ctttattcat atctccgcct tcaacagcaa aaaatatagc tattggcata gtttgagttt
     3721 ttccaggtat atttccagct atcataagtg tagcaccaaa ttctccaaga gctctggcaa
     3781 acgacagcac caatccacca attattccag gccatgctaa tggaactgca atcttaaaaa
     3841 atactttcca ttcattaaga cctaatgttc tagcagctga caacatatta ttatcaatct
     3901 gttcaaaagc tgaccttgaa gttctataca tcataggaaa agacaccact actgctgaaa
     3961 ttacagttgc tgtccaacta aatattaaag tcctatcgaa atttaataaa aactttccta
     4021 taaatccatt cttcccacat agtaataata aaaagaaacc tacaactgtt ggtggcaaaa
     4081 ttagtggaag tgtaaatagt ccatctataa gacccttcca cttacctcta tagttagcca
     4141 taatatatga cacaaatatt cctattacaa aagttattat tgttgacaaa atagatgtct
     4201 taagtgatat ccataagggt gaccaatctg ttcccatctt tccctcctta attttaaatg
     4261 gaaatagtga aactaataac tctattaatt tcactatttc cattttatta taatacacaa
     4321 ttattttcaa ttttcattta tttatatata atttatactt aattacacat aatttatttc
     4381 taagtttatt ctactttttt atatccaaat tcttcaaaaa tcttttgtcc tgactcactt
     4441 aataaaaatt cttcaaattt cttagcagca tctacgtttt tactagcttt aattactgaa
     4501 actggatatg taataggcga atgagttttt tcgtctgttt tttcaactac ttttatctta
     4561 tcattattga cagtatcact gaagtaaacg aatccaacat cagcatttcc tgattgcacc
     4621 catgctaaaa cttcttttac atcttttgca aatactagct tatcttttaa cttatctttt
     4681 aaatttaaat tagttaacac ctcatctgca tacttaccag ctggaacact ttcagcttca
     4741 ccaacagcaa ttttcttcac tttatctgta gtcaaatctt tcatacctga tacactactg
     4801 tcagcagaag aaataagaac taaatcattt tttactaaat ctttcatagt atcagaaact
     4861 aataacttct cttcatccaa agctttcatt tgtttttgac ctgctgaaat aaataaatca
     4921 caaggtgctc cttgttctat ttgttgttgt aatgagcctg atgctccata gttaactgta
     4981 agttttacat ttgaatcaac ttttttatat tcttcttcaa tctttgccat tgcttctttt
     5041 aaacttgcag cagccgatat atttaactct actgaatcac tacttttagt tgttttttct
     5101 tgttttccat catctttttt gtcttcatta ctattgcacc caactgtccc caaagttaaa
     5161 catgcaacta atcctaatat tcccaatatt tttttcataa caatctccct ttttgtaaat
     5221 ttgatacaca ttttaacatg ttttattatg ttttatcatg ttttgttata tatagtttaa
     5281 ttatatatac atgataataa attttcaagt aatttattta cactactttt aaatattttg
     5341 ctacattttc ctttttttaa tgttaaaata accttatatg gaggtggatt atggactcat
     5401 tatcatcttt gacacctgtc gaggtagctg aattattaaa gataacaaaa aatactgtat
     5461 atgaattaat caaaagaggt gaacttcctt cttataaagt agggaaaaaa ataagagttg
     5521 atactaaaga tgttgaagaa tatatcaaca gccaaaaaac tggtaaagta aaggatacac
     5581 atttcaacaa taaaaatata gatataaata ataatttcaa aattattatt tctggtcaag
     5641 atgtaatatt agacatttta gctaggtctg ttgaaaaaaa attggatgga ataagtacat
     5701 ttcgctctta tataggtagt tataatgctt tgtatgagtt atataatggc agagtttcta
     5761 tagcatcatc ccatctatgg gatttcgaaa cggatgaata taatagcact tttgttaaaa
     5821 agctattgcc aggaatacca tgtgtactta taaatctagc ttatagaatg caaggtttct
     5881 atgttgctaa aggaaatcct aaagagataa caacctggga ggatttaaca aaaccaagta
     5941 taaccattgt aaatagggaa aaaggttctg gaactagggt tttactagat ggtaaactta
     6001 gacttctaaa ctttaatggt aaacatataa aaggttatga taatgaagaa ttatcacatc
     6061 ttggagttgc aagtactgtt tctcgtggaa tagcagatgt tggtttggga aatgaaaaag
     6121 ctgctttaca agtaaataat atcgacttta ttcctcttca caaagagcgc tatgatttag
     6181 ttattaaaaa agaagactta caaaatccag tttaccaaac tataattaac ataataaact
     6241 ctcctgagtt taaagctgaa cttcaaggtc ttggcggata tgatttaact gatactggaa
     6301 aaactatagc aaaagtttaa gattaaaatc gcaatctatt ttataagatt tataattttt
     6361 cacaaaattt ataacttaaa cacgaaataa attgcgattt tagtgtctca aaaaatttat
     6421 gtctttcatt ttatcatcca atacaaaata taatataatt aattgtgcaa tcatattata
     6481 ttaatatata tttacataat agtatttttc tttaatgtag attaacatat gatatgtttt
     6541 aaacatcaat ccccttgctt tcttatatat atttggcaac acatatgagt ttaataaatt
     6601 atatacaatg ttgctttctt cttgtatttt ttcaaccaac aagttattta taaaattacc
     6661 tttcttattt tcaataatct cactccaatt tatcttaact tttaatatat atcttcccac
     6721 taggtataca cactctacaa taaataataa aaatacaatg cagtcagtac ctgttgtctt
     6781 atataaatta cccgatacta atggctttgc gaagaaatta taaaaattca aagtatcacc
     6841 cgtataatca aaaagtatta atagcactcc tagaactctc aatactttaa tattcaacat
     6901 gtttttatct ttattatttt tgtttttcat atatactacc tctcttttct ttagatttga
     6961 ttgtaatcta atactaactc ttttatttga ggtataaatg aatttaattg gtagttattt
     7021 tcatttttac tagaaattaa ttcacattac taaacaaaac ttatatttaa acataaaaat
     7081 agcctatcta tcttttaata aatacaaaaa taaataagct actaatattc aattaacttt
     7141 tatcacttaa aacattacta tctataaatt taaatatttt attgaagtat actgtactat
     7201 catgttcaaa tccatcaaca tgaccaatac cttctataag ccataggtct tttttatcat
     7261 gtttaactaa atcatacatt ttcttgctat tttgataatt acaaacagta tcttttgttc
     7321 catgtataaa cagtatgggt acattagatt tctcaactga ctcatatggt tttacatcac
     7381 tgtaccaaaa tccagattta aacttattat ataaatctcc acaagtcacc acataattga
     7441 ttaatatatc tggtattcgt ttttctagta ctcccattct aattgcatct ttcatttcag
     7501 aataaggaga atccagtata taaaaatcga ccttatcatc ttttgaattt atctctgaat
     7561 gcatagcagc tgtccctgcc cccatggaaa atccatgcac acctaatctc ccttcaggaa
     7621 atttattttt tacaaattta actagactat ctaaatcata cctttcataa agaccaaatg
     7681 tgtaattatc tccaccacta ttaccagtat ttctttgatt ataaactaat acattataac
     7741 ctttatctaa atatctatat gctaccttta acatttcata gtaactactt ccaattccat
     7801 gaacaagtat catagtatct cttgtcttta tatttgaagt tataaaaaga ctttcaagct
     7861 tgtaattatt agaactttgt atcattaatt cagatgtctt gtagtttttt aatttctcca
     7921 gtggtttatc ttttctctca gaaaatattt ttactatatc ttcgtttttt attaactgct
     7981 ctaaaccaac tgaactatta tacactaatt ttccaaatat ataagataca attaatagga
     8041 ttaatagaaa cacaattgat ataattataa atttatttct taaactaata caatttttaa
     8101 aactattttt catatttatt taccatacaa ttttcctata ttatattttc taagtaatag
     8161 cataatt
//
LOCUS       DAMKMI010000158         8160 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMKMI010000158 DAMKMI010000000
VERSION     DAMKMI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.164"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(119..970)
                     /gene="gatY"
                     /locus_tag="PC429_003269"
     CDS             complement(119..970)
                     /gene="gatY"
                     /locus_tag="PC429_003269"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF3848745.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            complement(1019..2188)
                     /locus_tag="PC429_003270"
     CDS             complement(1019..2188)
                     /locus_tag="PC429_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3848746.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIVEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            2658..3575
                     /locus_tag="PC429_003271"
     CDS             2658..3575
                     /locus_tag="PC429_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF3848747.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMQFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
     gene            complement(3704..4633)
                     /gene="pfkB"
                     /locus_tag="PC429_003272"
     CDS             complement(3704..4633)
                     /gene="pfkB"
                     /locus_tag="PC429_003272"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF3848748.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVSK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYYVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            4900..5625
                     /locus_tag="PC429_003273"
     CDS             4900..5625
                     /locus_tag="PC429_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3848749.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            5708..6862
                     /gene="nagA"
                     /locus_tag="PC429_003274"
     CDS             5708..6862
                     /gene="nagA"
                     /locus_tag="PC429_003274"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF3848750.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICKYKVNK"
     gene            complement(7561..8043)
                     /locus_tag="PC429_003275"
     CDS             complement(7561..8043)
                     /locus_tag="PC429_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF3848751.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
BASE COUNT         2877 a         1178 c         1030 g         3075 t
ORIGIN      
        1 agctgatatt tttcatcaat taaatgaaaa aaatacacca tttacatata attaatataa
       61 atttttcata taacaaatat cctaaactta tctaaaataa atttaggata ttttattttt
      121 atactctatt tacagaccca cacactttaa tcttttcttt cacaaaatcc tttactgatt
      181 gaagccctgc taatccatac tttttagggt ctataacctc agaatcttca tctaaaactt
      241 ttttaacccc tttactatat gcaattctta attcagttgc atagttaact tttgcaatac
      301 ctctttttat agcttctttt ataatatcat catgtattcc tgaacctcca tgcaatacta
      361 aaggtattga aactacattt ttaatttctg ataacctatc taaatctatt ttaggttcac
      421 ccttatacaa tccatgtgca gtacctatag caatagccaa tgaagatatt cctgttctat
      481 cgacaaactc ttttgcctct tttggatttg tataatcatt actattcccg ccatctaaat
      541 catcttcttt tccaccaact ttcccaagtt ctgcttccac tggtataaga gaaggactac
      601 atgcttttac tactgattta gatatagata tattattttc aaagctttca tgagaaccat
      661 ctatcattat tgatgtatat ccacttctta aagctttcat tgcaaggtca aaacttgaac
      721 catggtccag atgcattgct acaggtatct ttactctttc tgcagcaacc tttgcatttg
      781 ctagataata atcaagacct gcatatttta cagtagatgg agtagtttgt aatattactg
      841 gagaatttaa ctcctcagca gcttctataa cagcaattac catttccata ttttctacat
      901 taaatgctcc tactgcatac ccttcatctt gtgccttttt aagcattgat gttgttgtta
      961 ctaatgccac attgataccc tcccaaatta tttagttata atattttaat cttatctttt
     1021 aatattcata tattgttaca ccttttacta ctcgatttac tgttcctgta gctgatggcg
     1081 tatctggctt atttccaacc tttaccgata caaacaatgc tatagtttgt gcaaacaata
     1141 tgtatggcat tgcaagatac acatctggta atttattata cttttcttta aactcaaact
     1201 ttgttcctga gaagttattt tcattttcta ctgacactgc acaagtgaat ttagcaattt
     1261 tatccctttt tatctcctct aaaacatcta aatcatattt tcttgtatat aaacagttag
     1321 atacaaattc aaatacaagt gtattttcat caataaatga ttttggtccg tgtctaaacc
     1381 ccataggaga atcatacact gtagatattt ttcctgctgt aagttctaaa agttttagtt
     1441 gtgcttcttg tgtcaaacca cctaatcctc ctgaacctaa atatacaact ctatcaaaat
     1501 ctttatttat taactcatgt atttcatctt ttctatctat aacatttctt cccatttcaa
     1561 ctattgcatt tatatatgac ttttctacat catcttctaa actatcgaat attaacatag
     1621 cagataacat catacacgaa aaacttcctg tcatggcaaa gccttcatca tttgattttg
     1681 atggcattaa taacaagtaa ttattttcgt cgtttttagt catcttagct agttctcctt
     1741 ctggattaca agttatagct aaatgataaa aattatctac aatcttattc cctaaattta
     1801 aagcagccaa actttcagga ctatttccac ttcttgcaaa tgaaattaat aatgttggta
     1861 tatctttttt taagtaatca tatgggtttg aaactatatc cgtagtaggt attgcttcaa
     1921 atatgtattt tcttatgtca tttttatttt taaggtaagg aagtattgaa tttcctatgt
     1981 aagctgaagt tccagcacct gtaaatataa ctctaaactg cccatgatta ttacttattg
     2041 tatctatgaa tctacttaat ttttctttgt tagatttata tatttcataa gtctctaacc
     2101 ataattcagg ctgttgtttt atttcattag ttgttattat tgcacctaaa tcttttagtt
     2161 tttgttcttc taatttaaac attttcattc tcctttcata cttgtaacgt ttacatttgg
     2221 taatgaggtt ataaccactt taaactataa aaaaatatac tcttttataa ttttttgctt
     2281 agatttttag tactacttaa actcactata aaatatacac tatagatttt aattaatttt
     2341 atagtttatc tttaaaaatt taataaaatc atactctata tactttagta tttttatttt
     2401 acgataaaaa cttgttcaga ccaaagtggt aattaccact aatccaaata taaattaatt
     2461 tgaatttttt gtcaatacat aaaatattta tatcaatcaa ttttatagat atcctacaaa
     2521 attactttat caatatttct tatttcgcta atttacttat ataagcttta aatttatttt
     2581 ttgatacaaa gtttacaaat ttatgtgtct gaattataat caattctgta aatataaaaa
     2641 ccttaggaga aagtattatg aaaaatataa aaatgggaat gataggtctt ggctccatag
     2701 ctcaaaaggc atatttacct atattaacta agtctgagag atttgagttt gtaggagctt
     2761 ttactccaaa taaagttaaa agggaaaaag tctgtagtga ttatagaatc atgcaattcg
     2821 attctattga atccctagct aaaaaatgtg attgtatttt tctacatagc tctacagaaa
     2881 cacactacga aattataaaa atattattaa atttaggtgt tcatgtatat gtagacaaac
     2941 ctcttgcttc aacagtaagt caaggagaag aacttatcga attaagtacg aaaaaaaact
     3001 taaatttaat ggttggattc aaccgtagat tctgtcctat gtacaaagaa attaaaaata
     3061 atgctacaga aattgtatct atcaatatat gtaaacatgg attaaattcc ttaagaaatg
     3121 ttaaattcga cagtactctt atagatgact atatacatgt tattgacact gctttatggt
     3181 tagcaaatga agatgttgaa attagtggag aggatttatt tttaacagac aataagaatc
     3241 ttatatttgt aagtcataaa cttaaaggta aaaatttctc tataaacacc tctatgcata
     3301 gagattctgg aacaaagtta gaacaagttg aaattctttc aaagggtaag attcaaagag
     3361 ttaagaatct taatgtactg gaaatagaag aaggtggaaa cctaacttta aagcaaagtg
     3421 gtgcatgggt aaatatatta aaacaaaagg gttttgagga tatatcaaat cattttattg
     3481 attgtataga aaataatata aaaccagcta tcagtggaga agagtgtatc aaggctcaaa
     3541 gattactaga gaaaattata aattcagtaa aatagatatt tattataaaa ataggttaaa
     3601 aatgaatttt tctaacctat ttttatataa gactatatct tttatattaa aattaaaatc
     3661 tattacaaca acattaaatt tattatattc ccattaaatg tatttaactt aactttttta
     3721 cttcaactaa atcttgatat ttatcaattt ctcccaaatc tacaaatcca gtttccatat
     3781 ttaaagcatt acttatacca caagcatttg caaatttaag taaattttct attttataac
     3841 cccttaaaat acctacacta aatccagcca ctgtgctatc tccactacct acagtattta
     3901 tacactttac aactggtact tttacataat atacgttatc ttcgtttaaa tacaccatac
     3961 catcttttcc taaagatata catacattct ctgcacccat ttcaataagt ttttttcctg
     4021 acaagataat ttcctctctt gattctatat ttattcctaa taaatgtctt atttcatcta
     4081 tatttggctt tattaaatat ggttttgatt ttaaagctat ttttaatgct tctcctgatg
     4141 tatctaatat aaactttata ttatttgctc tacatatatt tcctatcttt tcataatagt
     4201 ctatcggcat attctcacaa taactaccag aagctaccag tattttagta ttttttagta
     4261 cttcctctat atcattttca aatctctcta aatcgctttt ttctactatt ggtccttttt
     4321 ctaaaaattc tgtactaact ttattgtcct caattatgtt taagcaattt ctagtttcct
     4381 gctctatttt tgtaaattta ttttgtatat ctagttttct aagttcattt tttataaatt
     4441 ctccattaaa tccgcctaga aatcccatag ctaaaggatt ttcttttaaa attttacata
     4501 ccttagatac gtttaatcct ttacctccag cagttgcatt cttacttact accctttgca
     4561 cctcacctat gtttatatta ttcactctat acattctatc tatagatggg ttgaatgtta
     4621 ttactgtaat catactagat tctcctcatt attaattttt acttttctaa acaataaatt
     4681 aatctgtaaa ataaaaaata cctcatataa tatatccaaa gtggttatta ccaatttgaa
     4741 tataaattat tttacttgtt ttgtcaatgt accataattt tataataaat taatagcctt
     4801 acttattgaa ttttgattaa actttattca ttgacataaa taattctata ttctataatc
     4861 aaattgtggt aataaccact tagaaaggac tatattttta tgataaaaag aaatgataaa
     4921 agaccaatat atgaccagct tgttgagata ttaagaacta agattgaaaa tgagatggaa
     4981 ccaaacgata gaatgctttc cgaaagaaaa atttgtgatg aatatggagt aagtagaacc
     5041 acagttagac tagctatggc tgagttagag catatgggat atatatacaa aagacatgga
     5101 aaaggaactt ttgttgctgc actaagtcaa aattctcaaa acttaatgga aagttatagt
     5161 tttacagatt atatgagaac acaaggaaaa aagccaggta caaaggtatt atcatttgag
     5221 gtagtagaaa gtacaaagta tttctctgaa aaattaggta taactcctgg tgaaaaaatg
     5281 ataaaaataa tgcgtttaag acttgctgat gaccttccta tgatgcttga aagaacatat
     5341 cttcctatga aagaatttat aggattgaca aaagaaaaaa tcgaacaaaa acctttatat
     5401 gaaatattta gagaagatta tggcgagatt attaaagttg ctgatgaaga attttcagct
     5461 ggaataatct cggataagga agctaagtta ttggaagtac caggagattc accttgtctg
     5521 aaattacttc gtaccacata taatgatgac aatatagtta ttgaatttac tttgagtgtc
     5581 gctagaagtg ataaatttgt atatagagta agacatgtaa gatagtaagt taaataactt
     5641 tcaagaaaac agaatgatat tataagcgtc aatgccacaa tgataatata attataagga
     5701 ggttattttg aaaagtttta tttatgcaga taaatttttt atgaaacatg gaatcaaaga
     5761 aaaaggttac cttagtataa ttaatggcaa attcggtgaa tttcaacaag aaagacctaa
     5821 tgttactgat aatgttatag atttttcagg taaatatatt gctcctggct tagttgatac
     5881 tcacattcac ggtcttttag gtgcagatgt catggacaat acatttgaag caataaacac
     5941 aatttcaaaa ggcttattaa aatatggagt aacatctttc ctacctacaa ctttaactga
     6001 ttctgtagat acactaagtg attctgtaga aaatataagc aatagcttca atcgagttga
     6061 gggtgcaaaa atacaaggga tttatttaga aggtccattt tttacagaaa aatataaggg
     6121 cgctcaaaat gaaaaatatt ttaaagaccc tgacatcaat atattaaaat tatggcaaaa
     6181 aaaatcgaaa aatttaataa gaaaaatagc aattgctcca gaacgtaaag gttcaataga
     6241 attaacaaga tatgcaacta aaaataatat atcagtttct cttggtcata gttcagctac
     6301 atttgagcaa gttaaacaag tcgttaattc aggtgctaaa gtgtttgtac atacatataa
     6361 tgggatgagt cctttaaatc atagagaacc tggaatggtt ggggctgcta tggtactaaa
     6421 agatacttat gctgaattaa tttgtgatgg tcatcatgta agcccaatag ctgttaaaat
     6481 aatgatggat gctaagtcta gtaataacat agctttaatt acagattgta tgagagctgg
     6541 tgctatggag gatggaaaat atactcttgg taagtttaat gtaaatgtaa aaaatagtgt
     6601 tgcaagatta aatagtggga gtttagctgg aagtgtatta actatggaca aggctgtgag
     6661 aaatatagtt gattggaaca tagccacttt agaagatgca ataaaaatgg ctacatatat
     6721 ccctgcttta agttgtaata ttgataatgt ttgtggctcc atacatcaag gtatggaggc
     6781 agattttata gtaacagata aggactttaa tatatacgaa acttatataa gtggtatatg
     6841 taagtacaaa gttaataaat aggttcaata taaggttatg gaggctgtct aaaaagagtc
     6901 tggaaatatt taaagttatt tctccaaaat ctgatttaac acttttattt ccatatcgtt
     6961 aataaactat tgcgatagag gttattttaa acaaatatat attataagta tttttgtgat
     7021 aatattttcg ataatatgta catttaaagt aacttttcta ttattatttg taagttttta
     7081 tcttgaacaa acatataaat ttttaataaa gcatgagata aaccctatat ttcaattact
     7141 aatgatgaga ttttaattga taacagatat attttcaaat gtaaaaatac taaaaatcat
     7201 atcaatttta aagatattgt aggtgtaaat aaacaagata atactataga aatttttaca
     7261 aatgaaagca cttattgtat atatctaaaa gttctaagca ttgagagcga agaatatcta
     7321 atagatagac taaaatcatt ttgttgattt tatatatttt atgttaaata taaagcagag
     7381 tagttaaaac taccctgctt ttgtaattaa aatttaaaaa taataactca taatacattg
     7441 aatttgagct taacagtaac tattgtaata atagtatcaa tcttttgtgt tgaatataat
     7501 ttatttacac tatagttaag catgatttct aatgttattt tctctaattt acaattatga
     7561 ttaaaattca ttaatatata tatatttata agttgtattg tttatattat ttattacttt
     7621 tttaaatcca ctagctgtaa taataaatgt cttttctggt atccctggtg aaaagattat
     7681 tttatcatgg ttaaatatat cttgatctat tacaagagag atatttttct catatccaaa
     7741 aggagataca catccaggca aacattgtgt tttttgtgtt aattcttctt ttgaacatat
     7801 agatactttt ccacctaaaa cattttttat agtttttgga tctaattttt ttccttctgt
     7861 ggttacaaat atatagtaat ttccagacct atcttttata aataaacttt tactttctac
     7921 tccagtatat tgtaattttt ctcttatttc tttagctttt tcaaatgata aaataggttc
     7981 atggtcatat tcttcaaacg ttatatgatt ttcttttaaa atatttttga tattgttgta
     8041 catatatttt cctttctata tttaaattaa tttatatata atattcaatt ataaagtgat
     8101 tttataattt cttcaaacta tcataaaaaa cattcacatg ttttaggaat aaattatata
//
LOCUS       DAMKMI010000159         8029 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMKMI010000159 DAMKMI010000000
VERSION     DAMKMI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.165"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..2471
                     /locus_tag="PC429_003276"
     CDS             <1..2471
                     /locus_tag="PC429_003276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848752.1"
                     /translation="NYNDKYIYAIYLLNIAINSYLNNTLNPNNHILTKLDKGMPLLYK
                     GQIVIFKNINNTHITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQT
                     KNVLSSLGFSDLKNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCS
                     YISSTGAETDYPGNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHIND
                     FERILNRKRIESVNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQ
                     KLWLDKLINKNICTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKI
                     LENTPFNLDFLEETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFER
                     IINELSDNNTKFNTLNKLVSSYKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQV
                     ISAEKFKDSKTIKNLIMFGFYDLKYLDILNSDLIINITLYLYNSELSKYNYYMNQINS
                     ARNKIEKNNKIYNLLDIKPFKPTYETNKLEAYSTDSPISDDDSMDEYISNYKFDINLN
                     EIILSLSINQGIYNSKKEHVTSFMISDNNEYIFCTKKAIAYSIDWDKKSVRKKKLSEA
                     KVNDWFLFVDDYLNEEGELVINVIQKLLELNLLPSHYLDDYKLTNSWKSELKNYISYS
                     KYNYNDVSNSLAVLGEKINPITIKNWVTNKRLIGPIYLKTFEKIFKVIDSKLDHTEVY
                     DATKSIRSLHTKIKKSIDTFVSDNFFSSKSDIIKDEVSAVVLSMLGNTNDYVTPVQIR
                     EIHSCDKYLSPHLVNKVLDLYSVY"
     gene            2489..6016
                     /locus_tag="PC429_003277"
     CDS             2489..6016
                     /locus_tag="PC429_003277"
                     /inference="COORDINATES: protein motif:HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDF3848753.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            6018..7931
                     /locus_tag="PC429_003278"
     CDS             6018..7931
                     /locus_tag="PC429_003278"
                     /inference="COORDINATES: protein motif:HMM:NF020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HDF3848754.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
BASE COUNT         3072 a         1003 c         1219 g         2735 t
ORIGIN      
        1 tcaattataa tgacaaatac atatatgcta tttatttgtt gaatatagct ataaactcat
       61 acttaaataa tacattaaac ccaaataatc acattcttac aaaactcgat aaaggtatgc
      121 ccctgctcta caagggtcaa atagttatat ttaaaaatat aaataataca cacataactt
      181 taggacataa aaataaaacg gatacaacac ttatacctat tgactcggct tatatgctta
      241 taacctataa tggtcattta gaagtcaatt ctatgggcaa aactccagca tctaagactt
      301 ttcaaacaaa gaatgttcta tcatctttag gtttttcaga cttaaaaaat atgactggag
      361 taataaatga ttctacactg attatattgc cttgtaaaga tgatatatct gacttagtta
      421 gtaatattaa aataaaagat attaataata cttataagtt tactgaacta tttccatgtt
      481 catatatttc atcaactggt gccgaaactg attatcctgg aaatcacgct aagcaaatac
      541 ctttacttaa atttacaact aatatttcta gtgcatttga aattattaaa tctgataagt
      601 ctataaaaaa tgtatttata ataggtgatt atccaattta taagcatata aatgattttg
      661 aaagaatttt aaatagaaaa aggatagaaa gtgtaaatat tgtaacctca aattcaaata
      721 taagcaatat attagattta ccaaatatag ataatctaaa tatatactct tggtctaaag
      781 atgttctgtt aacctatagc caagattttg aaaatacctc tacttttcag aaattatggc
      841 tagataaact tataaataaa aatatatgta ctacttctgt tgattctaat atctctgact
      901 tgatacatca ggctagaaaa tcactttatt acatatctaa gtatgatttt gatatttcca
      961 taaaaaactc acttatgatg tgtagttact ctcttcttaa aatcttagaa aatacacctt
     1021 ttaacctaga ttttttagaa gaaactataa agcttttaaa tttaaatata gcaactcctt
     1081 cagcgaagct agaaaatata aaatatttaa tttcaagtac tagcccagat ttagaattaa
     1141 gtgcattatt tgatgatgtt atagtcaaat ttgaaagaat tataaatgaa ttatctgata
     1201 ataataccaa attcaataca ctaaataaat tagtatcttc atataaatac aaaacattta
     1261 caccaaatac acctgttata ttgctacaaa aacaatatga agctattata cttaaaaatt
     1321 tattaaaatc tagaaatata aatttacaag ttatttctgc tgaaaaattt aaagattcaa
     1381 aaacaataaa aaatcttatt atgtttggat tctatgattt gaaatattta gatattttaa
     1441 atagtgattt gattataaat attacactgt atttatataa tagtgaatta tccaaatata
     1501 attactatat gaatcaaata aattctgcaa gaaataaaat tgaaaaaaat aataaaatat
     1561 ataacttact tgatataaaa ccttttaaac caacttatga aaccaataaa ttagaagctt
     1621 attctaccga tagtcctatt tctgatgatg attctatgga tgaatatata tctaattata
     1681 aatttgatat aaatttaaat gaaattatat tatctctatc tataaatcaa gggatttaca
     1741 actctaaaaa agaacatgtt acatcattca tgatttctga taataatgag tatatattct
     1801 gtacaaaaaa ggcaatagcc tattcaatag attgggataa aaaaagtgtt aggaaaaaga
     1861 aactttcaga ggctaaagtt aatgactggt ttttatttgt tgatgattac ttaaatgaag
     1921 agggtgaatt agttattaat gttatacaaa agctattaga acttaattta ttgccatctc
     1981 attatcttga tgattataaa ttaactaatt catggaaatc agaacttaaa aactatatat
     2041 cttatagtaa atataattat aatgatgtat caaacagttt agctgtttta ggtgaaaaga
     2101 ttaatcctat aacaatcaaa aactgggtaa caaataaaag attaataggt cctatatatt
     2161 taaaaacttt cgaaaagata tttaaagtta tagattctaa attagatcat accgaggtat
     2221 atgatgctac taaaagtata cgctctttgc acactaaaat aaaaaaatct atagatacat
     2281 ttgtatctga taatttcttt agcagtaaat cagatataat taaagatgaa gtgtctgcag
     2341 ttgttttaag tatgcttggg aatacaaatg attatgttac acctgtacaa atacgagaaa
     2401 tacacagttg tgataagtat ctgtcacccc accttgttaa caaagtttta gacttatata
     2461 gtgtttatta aataaatagg aggtaaatat gaattgtagt gaactaagag ataaaaccct
     2521 agatcttgta agaaaagaac ttcttggacc tggttcggaa gatctttgtg aaaatataaa
     2581 atatgaaata ataacagata gtccaataga gcgttattca acaggtattt tatatccact
     2641 taataacaat attgatgtaa gtaatacatc tgaatatgat ggtgaagata atgtatttac
     2701 atcagatgat acacaaaatg aactagatag agctataagt atgtctaatg aaactttacc
     2761 ttcttctatg ggtatgactt ttttcctaaa aaaagatgca gaagtaataa atttcagggt
     2821 aaattatgct acttatagaa atgctttata tacagactgt catgttaaat atactggtga
     2881 cgacttctat tctagcaata caattaatga atatgtagaa cttaggggat cattcctata
     2941 tcttaagcaa aaactaacat ctgagaaggt ttataattta ctaaaaaagc atgatttact
     3001 tgataatgct gaagctaaat ttgttttata caagttagct aaccaatgcc ctgatgatga
     3061 gaataagact ggtggttatg ttcgtgttcc acatgaaaac ttaatagtat caatagatat
     3121 ggataaaaac tatattaaaa aacctatatc taaagattta gatatagttt tatctaaaag
     3181 aatttattca aatgactttg ttagtattac ttgtatgctg gttaatagtc aatctgggcc
     3241 taagaaatat aataatacta ttttccagcc tgaactaatg gtattagctg aagataacaa
     3301 tgatttagag tttatagata tttttacagc tggatcagta aatactttta aaaagttaga
     3361 ttctgaggaa atatctttag acttacttta ccgaaataaa aaaacttttg ctactggtca
     3421 tggtgtagct atagatcaag atattcaaaa tgatgcttta ataaaacttt gtactgagtt
     3481 tattcctcat tttgaagtac ctaaattgga ctttaatgta gatgaattaa gtgatgtttc
     3541 tagagaatta ctgtctatga aaaatttatc agatatctct gatcttagta aagataagaa
     3601 aatatatttt ctttataagt ttgttgatgc ttatgatatt tggatagatg atctagaaga
     3661 agattcaaaa gatcttagtg atgtttttaa agatgtagca tatgagcata taaaaaactg
     3721 cagagatact aagtctagaa tgctagaagg tataaattta attaagtcta atgatgaagt
     3781 atacagagca ttttctctaa tgaatagagc tatacttatg caaagatgtc attctataca
     3841 tattacggat agactccctg gatctaataa ccttgactat atagattact caaatgataa
     3901 ttctgcttgg agaccattcc agcttgctta tatacttaca tgtttatctt cgatagaaaa
     3961 tcctgactct aaacatagag atttagttga ccttctttgg atccctactg gtggaggtaa
     4021 aacggaggcc tacttaggtc tatctgcttt tactatcttc ttaagaagaa taagaaacaa
     4081 agaaaatggt aacggtacag ctatcataat gagatataca ctaaggctat tggctgcaca
     4141 acaattcata agagcaagta tattaatttg tgcctgtgaa attattagaa aagaaaataa
     4201 gtctgaactt gggaaaaaag agatttctat tggtttgtgg attgggagtg atcaaactcc
     4261 aaactttaat gatgaggcag aagaaatgct taataactta acaggtaata acattaagtt
     4321 tagatctagt ataaatagtg aatatgagtt agataattta aaatcaaaat ataataaatt
     4381 ccaattacta aaatgtcctt ggtgtggaac aaaacttgtt aaagaatggg ataaaacttc
     4441 taaaaaagct gttggtgatt ggggctatag aattaaatct agacctaaga aattctactt
     4501 atgctgtcct gaagaagatt gttcatttga agatatgtta cctatacaag ttgtagatga
     4561 ggagatttat aataaccctc caacactttt atttggtaca gttgataaat ttgcaatgat
     4621 aacttggact acagaagtag ctagtctttt tgctttagat gatgataata gcaacttatc
     4681 tcctgagtta ataattcaag atgagcttca tcttatttct ggacctttag gtactatggt
     4741 aggtttatat gaaactgcac ttgatgctat gtgtagctat aagggtataa aacctaagat
     4801 tatagcttct actgctacta taagaaaagc acaaaatcaa gttaaaaatc tatataatcg
     4861 taatgtagct cagtttccac ctccaggatt aacatctagt aactctttct ttgttaaaga
     4921 gatgccttta agtaaaaaac gtggccgact atatgtaggt gttattccat ctggaaaaac
     4981 tcaagttact gtagaggtta gacttatgtc agcattactc caaagactta aaatgatgga
     5041 gaatgtagag ttgaatttat tggataaata ttggacacta gtatcttatt ttaatagtat
     5101 cagagaatta ggtaagtgta gcacactttt aaatgacgat ataaaagaca acatggaaag
     5161 attatgtaaa agagtctcag cccaccctta tcaatcatta aggaacctaa atgaacaaag
     5221 agagcttact agtagactta cttctactaa gataaataca actttagatg aactatctaa
     5281 tactcttact tctaataatt taactgtcga tactctatta gctagtaata tgatttcagt
     5341 tggtgttgat gttgatagat taaatttaat ggtagtagtt ggtcagccta agttaacatc
     5401 tgaatacata caagctagca gtagagttgg tagaagtaat gaaggactta tatttacatt
     5461 atataatcct acaaaaacaa gagatagatc tcattatgaa cagttcttta gttatcatca
     5521 atcattctat aaatttgttg aacctactag tattacacct ttctctgaac cagctagaga
     5581 aagaggcatg catgcagtat taataagtat gattagacat attttaggac ttcaagctaa
     5641 tgatcttgca agtcgatttt caaaaaatat ggatggtgta gaagatttaa aaaacttcat
     5701 aatagataga tctatcgata tatttagaga aaataatcaa aatgctgatg ataatatagt
     5761 aaatgaattt aaatctgaga tttcatctga attagatatt ataatggatt tatggcatga
     5821 taaagctcaa atatctgata aagataattt attgcattat tataagcgcc ctagtaaatt
     5881 tgataataaa atatataata atcttttaat tccttatgat aaggatgtta ttacaaatga
     5941 aaaacaaaca ttaaattcta tgagaaatgt tgataaacaa tgtactgttg aaattataga
     6001 attggaggaa aattaatatg tctataaaat tagatgataa aactcataat ttaagaataa
     6061 atcaagctac aagtgttttt ggaccaggag ctatgctaga ttttattgat caaacattaa
     6121 tgactgctca tccaaattat tggactaata caattaaaat acaagatgat aggcttcagg
     6181 ctacattgag ggttaatgaa ctaagaacac ctccaactat agatgataga tcttctgttc
     6241 cttttgttag gtttcctaga tggtactttt gtccaaaatg tagaaaattt atgcctatag
     6301 aagattggga gaaactttat aaaaatgctc ataaagataa acctatgaaa atacctattt
     6361 gccttacttg tagcacaaaa aagtctacta aacaattagt acctactggt ataattacta
     6421 tttgtgaaca tggtcatatt gatgattttc catgggtaaa atgggttcat tttagaaata
     6481 aatctggaag aaaagaaatt tgtgaaaatc ctcaaatatt aatgaagact ggaaaaagta
     6541 acctaggatt agaaggtata gaacttgaat gtagctgtgg agctaaagct aatatgaagg
     6601 gcgcatttat gcgtttaaaa tcttctaaat ctaatgatta tggtgtacct agtgaatata
     6661 aggaaatgtt taaatgctct ggtaattcgc cttggtataa taaaaaagta aataaatgca
     6721 ctaaatattc atttgcttct caaagaggtg cctctaatgt atattatcct aagatagaaa
     6781 gttcaattat tataccacca tactctgata ctcttaattc taaagtgcta tctagtaaag
     6841 gttatgatac atttaagaaa ttcttagatc gtgctattaa aaagaataag ttagaacgct
     6901 ttatggatga ggattttgag gactttgcca gtgatattgc taaagaaaca ggttttgata
     6961 ctgataaagt atccatagtt ttgaaagatt tattgccttt tgaagattct tcaccatcaa
     7021 caacaactag aaatcaatat agagaagaag aatatgaggc tttaattgga aatatagatg
     7081 ctaaatctaa aaaaactaat gactttacaa tagaagaaca aaatctatct gattatgata
     7141 ttccaaactt cagtaaaata gtgttagtaa aaagacttag agaagtaagg gcattaactg
     7201 ggtttagtag agtcaatcca cctgataata atatatttgg tgaagaagat atagatgata
     7261 gctgtaatga tactaaggtt ataagtttaa gatctagaga ttgtaaaaaa tctttttatc
     7321 ctgcatctga ggttagaggt gaaggtattt tcatagagat aaataatgat atggtaaaaa
     7381 gctggatttc atctaatcct aaaatacagg ataatgcatc tattttaaat tctagatata
     7441 ataaagatat taatgttaat gacaaagctt atagaaaaat tacacctaaa ttcttattac
     7501 tacatacttt agctcattta attataagag aacttagttt tgtttgtgga tatgctacct
     7561 cttcacttag ggaacgtata tattgtaata attccgaaaa tgaaaaagtt atgtctggaa
     7621 ttttagttta cactgcaagt ggtgactctg aaggtacttt aggaggatta gttaggcaag
     7681 gtatgcatga ttcattacca tctatattaa atagtgctct taatcgtgct ctttggtgtt
     7741 ctaatgaccc aatatgtatt gatagtactg gtcaaggtag aaattcttta aatctaggag
     7801 catgccattc atgtacttta ttgcctgaaa ctagctgtga ggagtataat ttattacttg
     7861 atagaagcat gttaattggt gatatggata atagagatat tgggttcttt aaaaatctaa
     7921 ttttaattta gtaataacta attttttatt tatataacta aaggttaaaa gttaatttta
     7981 tgacttctat cctttagttt tttgtattta aattattatt ttttgcaaa
//
LOCUS       DAMKMI010000160         7998 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMKMI010000160 DAMKMI010000000
VERSION     DAMKMI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7998
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.166"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            27..101
                     /locus_tag="PC429_003279"
     tRNA            27..101
                     /locus_tag="PC429_003279"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC429_003280"
     tRNA            116..200
                     /locus_tag="PC429_003280"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC429_003281"
     tRNA            210..293
                     /locus_tag="PC429_003281"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC429_003282"
     tRNA            317..391
                     /locus_tag="PC429_003282"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC429_003283"
     tRNA            416..492
                     /locus_tag="PC429_003283"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="PC429_003284"
     tRNA            502..577
                     /locus_tag="PC429_003284"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="PC429_003285"
     tRNA            586..661
                     /locus_tag="PC429_003285"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="PC429_003286"
     tRNA            664..752
                     /locus_tag="PC429_003286"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="PC429_003287"
     tRNA            756..831
                     /locus_tag="PC429_003287"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="PC429_003288"
     tRNA            838..911
                     /locus_tag="PC429_003288"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="PC429_003289"
     tRNA            926..1002
                     /locus_tag="PC429_003289"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="PC429_003290"
     tRNA            1020..1096
                     /locus_tag="PC429_003290"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="PC429_003291"
     tRNA            1105..1180
                     /locus_tag="PC429_003291"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="PC429_003292"
     tRNA            1188..1261
                     /locus_tag="PC429_003292"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="PC429_003293"
     tRNA            1269..1345
                     /locus_tag="PC429_003293"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="PC429_003294"
     tRNA            1358..1433
                     /locus_tag="PC429_003294"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="PC429_003295"
     CDS             1509..1922
                     /locus_tag="PC429_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848755.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="PC429_003296"
     CDS             2231..5662
                     /locus_tag="PC429_003296"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF3848756.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="PC429_003297"
     CDS             5904..7832
                     /locus_tag="PC429_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF3848757.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3136 a         1017 c         1598 g         2247 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctgta aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag ataatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca tttttgatga ataaaaaaat atataatact ttcttaaata
     7981 ttttaataaa aagaaagt
//
LOCUS       DAMKMI010000161         7960 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMKMI010000161 DAMKMI010000000
VERSION     DAMKMI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.167"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            155..1606
                     /locus_tag="PC429_003298"
     CDS             155..1606
                     /locus_tag="PC429_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF3848758.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1804..2445)
                     /locus_tag="PC429_003299"
     CDS             complement(1804..2445)
                     /locus_tag="PC429_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF3848759.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2919..4298
                     /locus_tag="PC429_003300"
     CDS             2919..4298
                     /locus_tag="PC429_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3848760.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFINLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4342..5076
                     /locus_tag="PC429_003301"
     CDS             4342..5076
                     /locus_tag="PC429_003301"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF3848761.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5165..6364
                     /locus_tag="PC429_003302"
     CDS             5165..6364
                     /locus_tag="PC429_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3848762.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAEKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6388..6870
                     /locus_tag="PC429_003303"
     CDS             6388..6870
                     /locus_tag="PC429_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3848763.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7188..7785)
                     /locus_tag="PC429_003304"
                     /pseudo
     CDS             complement(7188..7785)
                     /locus_tag="PC429_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2945 a          911 c         1351 g         2753 t
ORIGIN      
        1 gcaatatatt aaaaaaatta ttcattttaa accttgtaat ttgattttat aaatattata
       61 attaaattaa tgaattttta acaaggaatt attcattttt ttgattttaa gatttattga
      121 gaaaacaaaa ctaaataggg gggtaattaa tataatggat tttttcaatg gtattttaac
      181 gagtattaac aatgcagtag tatttgtaaa caattactta tggtcgtata tcttaattat
      241 aatgttagtt gtaattggta tttactttac tattaaaaca aattttgtac aattcagata
      301 ttttatagaa atgtttagac ttctgggtga tggtacagcg aataaaaatg caaaaaagga
      361 aggaaaaatc tcttcatttc aagcattttg tataagtaca tcttcaagag ttggaactgg
      421 aaatatagct ggaatagcaa ttgcagtggt agcaggagga cctggtgctg tattttggat
      481 gtggcttata gcattaattg gttctgcatc aagctttgtt gagagtacat tagctcagat
      541 ttacaaagtg aaagatggtc aagcttttag aggtggacca gcgtattata tggaacaagg
      601 tctaaataaa aaatggatgg gtattttatt ctcaatactt ataactatat gttatggttt
      661 tgtatttaat gcagttcaag caaatactgt ttcacttgca tttaataatg catttggaat
      721 aagtaaaatg gctatgggaa taggtcttgg aattttaact gcaatagtta tttttggtgg
      781 agtgcataga gttgcaaaag tttctgaaat aatagttcct gtatttgcag gattatatat
      841 attagttgct ttggctatag ttgtaatgaa tataacagag ataccaagtg ttatagcatt
      901 gatagttgaa agtgcatttg attttaaagg aatggcgatt ggtacattta tgggagttgt
      961 aatgactgga gtgaagagag gtctattttc taatgaagca ggtatgggta gtgctcctaa
     1021 tgctgctgca acagcacatg taactcatcc tgtaaaacaa ggtttaattc aatctttagg
     1081 tgtttttaca gatactataa taatatgtag ttgtacagca ttcatggtat tattatattc
     1141 aggttatagt agtagtggag caactggaat tgaattagct caagaagctt taactcatca
     1201 tataggacca gttggaaata tatttatagc agtttgtata ttcttgtttg catttagttc
     1261 tatagttggt aattactatt atggtgaatc aaatatggag tttatgagtg gaagtaagac
     1321 taaacttaat gtatttagag tttttgttgt aggtatggtt ttatttggtt cacttactca
     1381 agtggatgta gtttggaatc ttgcagactt atttatggca ttgatggcga taataaactt
     1441 aatagcaata gcacttttag gaaaatatgc atttattgct ttaaaagatt atacaagtca
     1501 aaaaaaatca ggaataaaag accctgtgtt tattgcagat gaaatagaag gcctagaaaa
     1561 ggtatcagag tggcataggg aattatctga ggaacattta ggataaaaaa tagtataaaa
     1621 ttataaaacc attttgaaag actagagtat gtatatactt tatatgaata taatattgaa
     1681 ttaatttata ttactggaat ttttaattcc agtaatattt ttttaattac aaggatatta
     1741 tgatgactta aagactgtgg aaaactcttt tttagttttc attattaata ttaattgaca
     1801 aatttagttt ttatttaaat ttcttaagtg catataaatc gtattttttg ataatcccat
     1861 taagtctgca actttaacaa cagaatcctt aattttaaaa ataccacttt catttaaaat
     1921 agaaacaata gatttatttt tatcattgct agatatatta gaatcatttt caacaattac
     1981 ctttgcatct tggtatgatt ttaaaattac ttcatcagta tcttctacga tattttctaa
     2041 aacaggagca ctttgcatag cattatgttc cggaatatat gaaccaatta ttcttgttag
     2101 tggtgtatta agataaaaat gtatgcagag tagaccgatt attttattat caactcccct
     2161 tatcactata tctgttgatt ttaaaggttc acctagtttg tttctattaa aataactgat
     2221 aaaatctcca tcagcatcat tttcgagttt attaatgagt gccaaatcaa aatcagttat
     2281 aatatctcct tcttttacag tattatattt accattagct attttattta ttgaatagtg
     2341 tactccatta aatttatata aaataaactc gtatccttct cctaaatacg cagataatcc
     2401 atccactaga ttgttataag aatcgaaaat atagttatca gtcatgttaa aagtcatatg
     2461 ttacctccta agaaatatct taatatcata ttaacatata aaatgaattt aataaaatat
     2521 atttcagtga aataataaat ataatgaaaa aaaaactatt gactgaaaaa tatttcagtg
     2581 atattattaa aatacagtaa ggcaaacgta ttattgacag aatatataaa atgttaaaaa
     2641 tagtatctag gaattaaaca aattaaagga ctagtgattt gaattttttt agtataatat
     2701 gtgacttcca attaatattt attaaacacc aataaaattg taaatatgct taatgatata
     2761 ctttagcgat gaagtgaaaa tattttattt ttatgattaa cctataggaa ttaatttagc
     2821 atactgttta gctagtaaat ctaaataaat tttagcttta gctgatatca tgttgaataa
     2881 agtttctatt ggattttgta taacaaagga gtggggagat ggcacaagat aaaattgtgg
     2941 ttgaaatttt agaaaacatt ggtggtaagg aaaatattaa ggaagtagca cattgtatga
     3001 ctagactgag aataaaggta tgtgatagtt ctcaggtaaa tcaggaaaaa ctaaagaaaa
     3061 taaaaggtgt actaggtctt gtattaaaag gtgagcaata tcaaattgtt ataggtccta
     3121 atgtatcaca agtgtataat actcttaatg aagttatagg aattatacct gagaaggata
     3181 gtaaaaaaac aatatctgaa ggtaaacaaa atagtattgc aaaaatatta gatactattg
     3241 caggtatttt taatcctata gttccagcat tagcgggtgc aggtctaata aaagctatat
     3301 tagcattact agtagcatca aagtttttat ctgcagatag tgaaacctat ataatattaa
     3361 atacaatatc tgatggtgta ttttcatttt taccatttat gttagcttac tcagcagcta
     3421 aaatatttaa aatgaatact tatgttgctg tagcaatagc aggagcaatg ctacatccta
     3481 atttcattaa tttaattcaa caaggcttaa ctaaaatatc ttttatcggt attccgatgt
     3541 cactaatcca gtataacgga agtgtatttc cgatagtact aactatttgg tttgcatcgt
     3601 atatagaaaa aggagtagat aaaataacac caaaggcact taagataata ttagtacctg
     3661 cattgactat tttatttgca gctcctatag cattaattat tattggacca atagctcaaa
     3721 tgctaggaaa ctatatagcc ggtggagcaa gtatcctatt tgaaagagga ggaatatttg
     3781 cagggttaat ttacggagga atttattcat tcatggtagt aacaggatta catcatggta
     3841 tggttcctgt attagtagat ggaataacaa gatatggatt taactatata agtcctgtgt
     3901 caggtagttc aaatatggct caagcaggat ctgcatttgg agtatggtta aaagcaaaag
     3961 ataaagattt gaaggctgta gcagctacag ctaccatatc agcattaact ggagttaccg
     4021 aacctgcaat atatggtgtt aatttaagat taaagaaacc gtttttatgc gctgctatag
     4081 gtggtgcagc aggaggtgga tttgctgcat atttccaatg taaagcattt gctatgggag
     4141 gtccatcgtt tttaactctt cctatgttta taggagaagg tggacatgta ggattggtag
     4201 caatagcatt tacaatagca tttatagtat ctgcaatagc tacctatata gtaggatttg
     4261 aagaagaggt aacagcagat tcacacgcta acttataata aagagaaatt atttaacata
     4321 tcaaaaagga gattaattac tatgaaaatt tatttaacta aagattatga agaaatgagc
     4381 caagtggcag cacaaatggt gttaaagtat atgtacagca atgataataa aagaataaac
     4441 ctttctataa cagcaggtca aacaccaaaa agaacatacg aaatattaag tgaatttgtt
     4501 aagaataaac cttattttga aaatgtacat tattataact ttgatgaaat accaataaaa
     4561 ggcgaaaaag ttggagttac tataagaaat ttaactaata tgtactttag agaagctcaa
     4621 ataccagaag aaagaataca tgtgttagat gaaaataatt atcaaggctt tgatagaaag
     4681 atagaagccg atggtggaat agacatggta ttaatgggat taggttggga tggacacttc
     4741 tgtggaaatc ttagtggtac agtcaactct tttgatgaag agacaagagc tgtttcaact
     4801 tatataacag atgatatggc taaactatta acaaaatctg taggtggaaa tccagaaaaa
     4861 ttatttgatt actatgtgac atatggacct aaaacaataa tgcattgtaa aaatataata
     4921 atgatagtaa acggaaaaca caaagctcaa atattaaagc aaacactatt tggaccatta
     4981 gatttattat gtccatcatc tttattgaga ttacatccta acttcacatt aatagtagat
     5041 gaagaagcag catctgaaat aaaagattta atataaataa gtaaggaatt aaaattaaaa
     5101 atgaatttag gtgcaaagta atattttatt ttgcaccttt gctttaataa aaaggagaat
     5161 aaaaatgtat aatcatatga aaaaagtatt ccaaggatta gagggtgggt tattttcaag
     5221 tgtagagaaa gctgatgtag gcgattctta tcaaaaatta ggtgagcagg gtgtaagttt
     5281 aatgggatgg gcagatccat ttttcccaga ttactgtata cctagacata tattagatgc
     5341 agctacatta gcgatatcaa gtaatatgtc aactcattat acagcgcctg taggtaatga
     5401 cttattaaaa attgagatag cagaaaaatt aaaagaagta aataatttag atgttgattt
     5461 taaaagaaat atattaataa ctcctgggtc agactcaggg ctttattact caatgttacc
     5521 gtttataaat gaaggggatg aggttcttat accaacacca tgctatccaa ataatataca
     5581 aaatactaaa ttaatgggag gagttcctat atttgtagag ttaaaggaat cagatggata
     5641 tcaaatagat ataacagaat ttgagaagag attaactaat aagacgaagt tagtagttct
     5701 tactcatcct aataatccaa caactacaat atttaataaa gaatctttag aaaaattagc
     5761 taaatttatt atagacaatg atttaatatt aatatgtgat caagcatttg aagattttac
     5821 ttttgaaaat gaattaataa caccagcttc attagaaggt atgtttgaaa gaacagtaac
     5881 aatattttca acatcaaaag gtatgggatt aagtggattt agagttgcat atatggtatg
     5941 cagcgataaa ttaatggaca aatactatgg agcagcagtg acagtattag gtgctactaa
     6001 tacagtagct caaatgtcaa cgattgaagc atttaaagat agaagcttta tggaggattt
     6061 tgaaagagtt tttgataaaa gaaggcaaga agcatataag ataatcaatg aaatacctaa
     6121 tgtatctatg cagatgcctg agtccggatt tttaggttgg ataaatgtat caaagcttgg
     6181 aacatcatca gaaatacaaa agtatctagt tgacgaggct aaagtttgta ttaatgatgg
     6241 aattaattat ggtccaggag gagaagggca tttgagaata attttaggaa gctataaaga
     6301 cgatagtaag gtaattggtg cattaaatag aataaaagat gctttaatta aaaagcaaca
     6361 ataaataaaa aataattgga gtggagtatg ttatcaatat ttaagagtaa aaaaaatgac
     6421 aacaagctta aggcatttgc atcagggaaa gtaattccta tagaagatgt tgatgataat
     6481 gttttttcat ctaaaatgtt aggtgatgga atcgctattt atccaactaa tgaatatgtt
     6541 gtagcccctt gtgatgcaga agtaaatata ataatgaaag atactaaaca tgcagttgga
     6601 attaaattat ttaatggtat ggaaatctta tttcatgttg gaatagatac tgtatctatg
     6661 aatggagaag gttttgagat atttgtttca ccaggtgaca agataaaaag aggtgataaa
     6721 ttaatatctt ttaatagtaa attaataaaa gaagaagggt tacaagatgt agttataatg
     6781 gtagttacta attcagaaca gtataaaaat ataaaatatc atattggact ggaggcagta
     6841 atcgatgata ctattattgc agaaatttaa aaacagttat ttaagtaaat acttagataa
     6901 tttagatata gttaaaatta aatccttaaa atcaaattat ttaatttttg tgtaatttta
     6961 actataaagc cgattaataa tataaagtat actaaataat atggttatta tgattgttac
     7021 tgacaaagcc taagtagata taacaatttg ctctaattga gtattgctat atctacttgt
     7081 ttttatattt aaaataaaag agtctgcatt gaaaagataa ttctgatgag agtaaatagg
     7141 gtatttaata atagtttaat tctgacttat caagatgaac ctattcatat acctaaaaca
     7201 tctttaacac cttctaaatt catacaaata gctgtatagg ctgccttatt gattattttt
     7261 ccgtcaattt ttactttgta gtgaacagca tatataaata ttatcgaata gattgtatca
     7321 agagctctat tttgctattc attagctaaa gatagtactc tgtcagttat ttttgaaact
     7381 aatgcatgag atatatcaat tccgtaaata ttatttaaat gtatgatata tatcgcgagt
     7441 cgacattcct tttgagtaca tgccaattac ttggttctct attccagaaa tttcattttc
     7501 gtaattttga ataaccctag gctggaaatc atccctaggg atatttaatt ttacctctcc
     7561 aaaatctgat ttaacattct tagctctata tccatttctt gaatttgatg ttgctttact
     7621 ttggttatca tatctctcgt acattaaatg atcttcaatc tctgactcta acattttgtg
     7681 aattgtgctt gcgaataaat cttttaacat ctcttggatg tctttagtat tttttacatc
     7741 atactcttga actaagtgtt ttaatattta atttttgttg tacataaaaa atgtgtacct
     7801 cctattttgg taattagatt taatatcttg acttttagga gaatacacat tttattttac
     7861 agaccctctt ttctagaaaa gtttttatga aaattgtgat ataaattatt aataaaaaat
     7921 aattttatat ttattttatt atgtaattta gttttattaa
//
LOCUS       DAMKMI010000162         7741 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMKMI010000162 DAMKMI010000000
VERSION     DAMKMI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.168"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            314..1507
                     /gene="nifV"
                     /locus_tag="PC429_003305"
     CDS             314..1507
                     /gene="nifV"
                     /locus_tag="PC429_003305"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF3848764.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1532..3457
                     /locus_tag="PC429_003306"
     CDS             1532..3457
                     /locus_tag="PC429_003306"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF3848765.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3479..4474
                     /locus_tag="PC429_003307"
     CDS             3479..4474
                     /locus_tag="PC429_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF3848766.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4800..5273
                     /locus_tag="PC429_003308"
     CDS             4800..5273
                     /locus_tag="PC429_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3848767.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5307..5801
                     /locus_tag="PC429_003309"
     CDS             5307..5801
                     /locus_tag="PC429_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3848768.1"
                     /translation="MIRKANMNDLESIMQIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5898..6419
                     /locus_tag="PC429_003310"
     CDS             5898..6419
                     /locus_tag="PC429_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3848769.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6608..6919
                     /locus_tag="PC429_003311"
     CDS             6608..6919
                     /locus_tag="PC429_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF3848770.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6947..7633
                     /locus_tag="PC429_003312"
     CDS             6947..7633
                     /locus_tag="PC429_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3848771.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3105 a          761 c         1496 g         2379 t
ORIGIN      
        1 aaattttagt aggaatttta taggtttatt tttaaaggtt ttttgaaaat aaatctattt
       61 aaaaattaaa ttaaaatgca agaaaaacat aataactttc atttttaaga agttttcaca
      121 aaaaagaaaa aatatagtgc aataaatgat acaaagtaat ataatagatt acaagatatg
      181 aaaagctaaa aaaataaatt gcaaatgaaa ttaaaatgaa taaaaaataa aaaaataatc
      241 ataagttaga ggtatcaatg aaaaataaga cttaatgttt taaaattaaa agtatactaa
      301 ggggggcatt aatatgtgcg ttatatcaaa agatagagca aaggaaataa aaatagtaga
      361 tactacttta agagatggag agcaaacagc tggggttgta tttgcaaata gagaaaaaat
      421 aatgattgct gaaatgttaa gtgacctagg tgttgaccaa attgaagttg gtatacctac
      481 tatgggtgga gatgaaaaaa atgtaataaa gcacatatgt agtagaaatt taaaatcaga
      541 tataatggct tggaatagag cagtaataaa agatgttgaa gaatctattt cttgtggtgt
      601 tgatgcagta gctatatcaa tatcagtatc tgacattcat atagaaaata aattgagaac
      661 ttctagaggc tgggttttag aaaatatggc taaaacagtt gaatttgcaa agaaaaatgg
      721 attatatgtt tcagtaaatg gagaagatgc ttcaagagca gatatagact tcttaactga
      781 atttataaat gttggaaaac aagcaggggc agatagattt agatactgtg atacagtagg
      841 agttatgaat cctttttcta taaagaatgc aatagaaact ctttatgaaa gaactaattt
      901 tgatatagaa atgcatacac ataatgactt tgggatggca actgcaaatg cacttgcagg
      961 aatagcagca ggagcaaatt atgttggtgt tacagtaaat ggattaggtg aaagagcagg
     1021 aaatgcagca ttagaagaag ttttaatggc acttaagtgt gtttataaat gtgatttaaa
     1081 taatatagat actagaaaat ttagaggaat atgtgagtat gtagcacagg catcaggaag
     1141 aatacttcct acatggaaac cagtagttgg ggataatatg tttatacatg aatctggtat
     1201 acatgcagat ggagctttaa aagacccaca taactatgaa ccatttgacc caagtgaagt
     1261 aaatcttgaa agaaaaatag taataggtaa acattcagga agagctgcag ttgtaaataa
     1321 gttgagtgaa tatgaaatgt atatttctcc agaaaacgct accaagctat taaatgctat
     1381 aagagctaca tctataagat taaaaagaag tttaatggat aaagaaattc ttcaattata
     1441 ttgtgatata ctagcacatg agaaaggcac aacagaagaa gaagcagtta gaggttcata
     1501 tatttaagat ataattgttt aggaggaaaa tatgggagat aatatagttt ataaaataat
     1561 taaaaaacat atagtagatg gagaagctgt tgctggaagt tcaattggta taaaaataga
     1621 ccaaacttta acacaagatt ctactggaac aatgacatat cttcaactag aagctatggg
     1681 aatagataaa gtaaagacta aaagaagtgt ggcatttgta gaccataata tgttacaaca
     1741 aggatttgaa aatgcagacg accataagta tatacaaaca gtggcagata aatatggagt
     1801 ttatttctct aagccaggaa atggtatttg tcatcaagta tttttagaaa gattttcaac
     1861 accaggagat acacttttag gttctgatag ccacacacct actgctggag gtgtgggcat
     1921 gatggctatt ggagctggtg gattagatgt tgctttagcc atggcaggag gagcatacta
     1981 tataaaagca ccaaaagtct gtaaggtgaa tctagttgga aaattaaata atatggtatc
     2041 atcaaaagat ataatattag aagtgttaag aaaacaaact gtaaaaggtg gagtaggtaa
     2101 agtttatgaa tatggtggag aaggtgtcaa aagcctatct gttccacaaa gagctacaat
     2161 aacaaatatg ggggctgaac ttggagcaac tacttcaatc ttcccaagtg atgaaaaaac
     2221 actagaattt tttaagagtc aaggaagaga agaggcatgg atagagctaa aaccagatgc
     2281 agatgcagtt tatgatgaag agattactat aaacttggat gaattaaaac cattagctgc
     2341 aaaacctcat agcccagaca atgtagatga agttgaaaat ataggcaaga ttaaaataga
     2401 ccaagtggca ataggaagtt gtacaaattc ttcttatgaa gacttaatga aagtagcaca
     2461 aatcctgaaa ggaaataaag tacataagga tgtgagtttg gtaatagcac caggttcaag
     2521 acaagttatg gagatgattg ctagaaatgg ggctttggca gatataataa gtgctggagc
     2581 tagaatacta gaaaattctt gtggtccatg tattggtatg ggtcaatctc caggtactga
     2641 ctctgtgtct ttaagaactt tcaatagaaa tttctatggt agaagtggga ctttgtcagc
     2701 acaagtatat ttagtaagtc cagaagtggc agcagtaagt gcaatcaagg gagtgcttac
     2761 agacccaaga gaatttgata ttaaatttac aaatttagat gtaaatgaat ttttgataga
     2821 tgatagcatg ataattaaac ctgctgatgt aggaagtgat gtagaagtag ttagaggacc
     2881 taatataaaa cctttccctc ttaatacaga gctaagtcag tctataggtg gaaaagttat
     2941 attaaagaca gaagataata taactactga ccatataatg ccatcaaatg ctaagttact
     3001 tccttttaga agtaatatac cgtatcttgc aaactattgt ttcaatactg tagatacaga
     3061 atttcctcag agagcaaaag acaataatgg tggatttata gttggtggag ataattatgg
     3121 tcaaggttca agtagagaac atgcagcttt agcaccatta tatctaggtg ttaaaggggt
     3181 aatagttaag agctttgcaa gaatccacaa ggctaacttg ataaatagtg gtataattcc
     3241 aatggagttt tgtgatgaga aagattatga aaatatatct cttttagata atctggagat
     3301 accaaatatt ttagacaatc taggtagtgg aattttagaa gttaagaata caactaaagg
     3361 aacttctttt aaggtcaaag tagaattgtc agcaaaagaa gttgatgtat taaaagctgg
     3421 tggaaaatta aactacacta aaaatcaagc taattaatat gaaaagaggg aatattgaat
     3481 gtataaagtg acacttatac caggagatgg aataggacca gaagttgcga aagctatgaa
     3541 aaaggtagta gaagctactg gagtagaaat agaatgggaa gaagttaatg caggagaggc
     3601 agttattgaa gagtatggta ctccacttcc tgaatatatt atagatagta taaaaaagaa
     3661 caaaatagct ataaaaggcc caattactac tcctgtaggt aaaggattta ggagtgtaaa
     3721 tgtaacttta agacaggcat tagatttgta tgtcaattta agacctatta aaagttttaa
     3781 ggggataaaa tcaagatatg aagatgttga tttagtggtt gtaagagaaa atacagaaga
     3841 tttatatgct ggaatagaac ataaaatagg tgattatgca gctgaaagta taaagataat
     3901 aacaagaagt gcaagtgaaa gaatcgttga ttttgcatgt aactatgtta aagataataa
     3961 gagaaaaaaa gttacagcta tacataaggc aaatataatg aaaatgtctg atggattgtt
     4021 tttagatgtg tttagagaag ttgcatcaaa acatggtgta gaatatgatg atttgattgt
     4081 agatgcagca gctatgaact tagttttaaa tccagaaaat tatgatgtaa tggtaatgcc
     4141 aaatctttat ggagatatac tatcagattt aggtgctggg cttgttggag gtttaggtat
     4201 aattccaagt gcaaatattg gtaaagattg tgcgatattt gaagctgttc atggaagtgc
     4261 tccacaaata gcaggtcaaa ataaggctaa tccaacagca cttattcaat catcagtaat
     4321 gatgcttaga tacttaggtg aatatgaaaa tgcacaaaaa atagaaacag ctttagaaaa
     4381 agtatttctt gaaggaagta aattgactgt agatttgggt ggctcagctt ctactacaga
     4441 gtttgcggat gaggtgtgta agtatattgt atagatttta tttagggaaa aagtaaaata
     4501 tagattgaag gaaagaggct ttctcaaaat agagataagc cttttttatt ggaatgataa
     4561 aatatatttg attttataca aaagagagtt cctcataaac gaaaaagtta attttgggat
     4621 atccatttta attttaaaat taattgatta atatgcaaag aaagattaaa taaatttatt
     4681 atttttaatt gaactttcaa ccaaaatatg gtaaaattta aaaaagttga actttcaact
     4741 aaaaatgaat atattgatta tgtgaaattt tataaaacag aaactatttg actttagcca
     4801 tagtagtttc agtggaggtg tgtttgatta agactttaga tagtaatata ttaagagaag
     4861 taggtacttt atctagagca gtaaattcaa taaatgatat taagtacaag gaattaaagt
     4921 tacaaaaagg acaatttaca tttttgacaa gaatctgtga aaatcctggt ataaatcttg
     4981 tggaattatc aaatatgtta aaggtagaca aggctacaac aaccaaggct atacaaaaat
     5041 taataaaggc aggatatgta gataaaaagc aggataagtt tgataaaaga ggatataatt
     5101 taactccaac tgataaatct ttggaagttt atgagttgat aattgaagaa gagaatagaa
     5161 gtatagagat atgttttgat aattttacag atgaagaaaa acaagtggta actaagttat
     5221 tagaaaaaat gagcaaaaac gtagaaaatg aatggtttaa agtaaagaga tagtaaataa
     5281 atacattaac ttaaggagat atttttatga ttagaaaagc gaatatgaat gatttagaaa
     5341 gcattatgca aattataaaa tctacagtgg aagaaatgaa gacttataat aatacacaat
     5401 gggatgaaaa ctatccatta gaaaaagact ttgtttcaga tattaagaaa caagatttat
     5461 atatttatga agtggatgga gaagtagcag ggtttatatg tcttaattat gaagagccag
     5521 aagaatattt aggcttaaat tggtcatcta ataaaaaagc aatggtaata catagaatgg
     5581 ctgtaaatcc aaactttaga aagacaggaa tagcatctaa gttagttgat tttgctgaaa
     5641 aattagctgt agaaaacaat gtatcatact taaagagcga tacatattca ataaactcaa
     5701 aaatgaattt attacttaca aagtgtggtt ttataaaaat cggagaaatg agttttttag
     5761 gaaaggagaa atctttctac tgttatgata aaatattata aagagcattt ataatacaag
     5821 ggataaataa tagattttca gcattagtaa gggcataata taacctagtg tatgctatta
     5881 tgaaagggat gatttatatg attggatttc taaagaaaag aagaagtatt agaaaataca
     5941 aggatgttga agtagaaaaa gaaaaattag ataaaatatt aaaagctgct cttttagctc
     6001 catcatcaaa ggggcttaga acatgggaat ttatagtggt agatgataaa gaaaagctaa
     6061 ttaatttatc tcaatgtaga actaaaggtg gtggattttt cttaaaaaat gcacctttag
     6121 ctattgtaat tattgcagat aaagaaaaaa atgatgtttg gatagaggat gcaagtatag
     6181 ctgcttcata catacaactt caagctcatg aactaggttt aggttcttgt tggatacaag
     6241 taagaaatag aatgcatgat gataatatag aagctgataa gtacattaga gaagaattaa
     6301 aagtacctag caagtacagt gtagaatgta taatttctat aggatatcct gatgaagaaa
     6361 agaaagcata cggtgactct gatttagact ataaaaaggt tcattttaac aacttttagt
     6421 atataatatg ttgtattaag acattatatt tttttctatt gttaatatat acttaaaatt
     6481 gtaaaaatat atctgaattt tactaactta tctaggggtt atgtacaagg attttaaaga
     6541 taaatataga aattaatgtt tataatcgat acataacttg agtaacaaaa tttaaggagg
     6601 ttaaagtatg ggtatattta aaacaaaaat ggatgaggac tggaaagtaa actacattaa
     6661 agagtttaat gaaatgagag attcttatga gagtaaactt caaaaaaaac agtttgaggt
     6721 agatagtttg aaatcagaat tggatagact aagaagctat aaaaatagcc ttaagccaaa
     6781 agaaaaacaa attactgatg atgatataaa taatataaag agtttaagaa gagatggctt
     6841 aagctataaa gaaatttcaa atcaaacaag ttggagcaaa gcaactgtaa gtagggtatt
     6901 aaatggactt tacgattagc atatacatgg ggtgatttaa attgggatga agtatagtta
     6961 caaaaaatgc ataatagatt ctattttatt gatgtttata gttcaaatat taaggatgat
     7021 tttgaattat gtacttctaa gtcaatttga atttactctt gaaaatttta atattataaa
     7081 cttaatttct tttacattag ttggtttatc gttaatttta tttttaaaag ataattcttt
     7141 gtataacaag gttagaaata gaaaaatcac agaagctttt gaagaaaata aaaacaatat
     7201 acttattgaa aagtgtaagc tgatactttt tgttgtggtt ttatctttag caataattgt
     7261 aacttattgt actaagggat atgtactttt taatgtaact atgatgactt tgtctgtatt
     7321 gatagtccca atatttgaag agttattttt tagagaatat atatggaact atctaagtaa
     7381 ttttataaag tcaaagagta aaattatctg tataacatct atactatctg gaatttataa
     7441 tataggatac atagatgtta taagaaatta tgtaatattg tataacaatt caagctacac
     7501 ctttgaagtt attatttcaa aaattatgat aggtactgtt tttggtattg tacttggatt
     7561 agttaagtat aggtttagag atgtagggtt ttgtatatta ttgaggagct tatttgctat
     7621 atttattagg tagatagtat aatcggttta taaggttgtt ttaaatttaa ttttaaatca
     7681 accttatttt atttgtgttt tataaagcac ttatatatta ataactaata aatttaataa
     7741 a
//
LOCUS       DAMKMI010000163         7738 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMKMI010000163 DAMKMI010000000
VERSION     DAMKMI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.169"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            100..2328
                     /locus_tag="PC429_003313"
     CDS             100..2328
                     /locus_tag="PC429_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF3848772.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2566..2865)
                     /locus_tag="PC429_003314"
     CDS             complement(2566..2865)
                     /locus_tag="PC429_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF3848773.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3072..6113
                     /locus_tag="PC429_003315"
     CDS             3072..6113
                     /locus_tag="PC429_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF3848774.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6274..7548)
                     /locus_tag="PC429_003316"
     CDS             complement(6274..7548)
                     /locus_tag="PC429_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3848775.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2862 a         1077 c         1104 g         2695 t
ORIGIN      
        1 attaagacaa aattttaact ttactatttg tgtaaaaagt aatataattt aaaattgatt
       61 ataaatctaa cttttttctt tatcaaaata ttttaggagg tgagacattt gaaaaaaact
      121 aatagtttaa aagaaatttt aaactggaat aatatcaaga atttaagacc ttcaaagggt
      181 ttaaaaaata tattagaaat ttttaaaagt gacctaaaag atatacgtgg caattatgcc
      241 gtacttgtaa tagttttagg tcttgctttt ttaccatctc tgtatgcttg gttcaatata
      301 aaggcatctt gggaccctta tggaagcact gctaatatgc ttgtagcagt aacaaatgaa
      361 gataaaggaa atgaaatagc aggacaaaag attaatgtag gagaccaatt aattgaacaa
      421 ctcaaagata acaagagttt aggttggcag tttgtagatg aaaaaactgc tcttgaaggt
      481 gttaaaaaag gtacttatta tgcatcactt aagattccaa aaaattttac aaaagatttg
      541 acatctttaa ttacagatga tgttaaaaaa ggcgaaatta tctatactgt taatgaaaaa
      601 ataaatgcag ttgcaccaaa gattacagat aaaggtgctt ctacaataca actacaggtt
      661 aatcagacta tagtaaaaac agttagtgaa atcgttcttg gtatatcaaa cggagttggt
      721 gcaggaattg aacacagtct tccaaaattg acaaaagttg aaaattccct tattgacctt
      781 caaaagagat ttgaccatat aaataagact gttgatttag cttctgatgc tacttcaaaa
      841 ttatctgata cagttaaaga tattaaatct gatttaccta caattaaaaa gactctgaat
      901 gatacaaaac tattatcaag tgacttaaaa aaattcttag aggatacaaa tgataattta
      961 gacgaacttt ctcctcttat taagtctgac ttaaatttaa tggttgacct ttcatcttct
     1021 gcttcttctc ttacattaaa tttaattgat gcagtaaata gtggcagtga agatgtacct
     1081 aagttgatag ataatttatc agaaaaactt tctaatttgc aatctttaaa tgatactttg
     1141 gtggattttt taactaaatt aaaccaactt acatcaaata atagattgga tgatgtcata
     1201 gataatcttg aagactctag caataaaata gattcatcta tatcaacatt aaatgatatt
     1261 aaaaacaaag ttatatctgg acaacaacca tctataagtg ctttgaataa tgttttaagt
     1321 ctatcaaatg gtattggaag aattaattta aatattttaa ataattttga ctcaaaaata
     1381 tcaaaaccta taaatagtat atttgcaaac agtataaaag tagcaaatga tataattact
     1441 gttttagata aggcagaagc aaaacttcca aaggttgaag aaatacttac tacttctcta
     1501 aaactttcag gaaatgcaca ggaaagtata tcattgataa gagaaagact tcctttagca
     1561 aaaggaatgt tggatgattt gattgatact ttaagcaaaa ttagtaatgg tgaagatatg
     1621 aaaaaattag tcagcttgct tgaaagtgat atattgacaa agtctaattt cttaaaagaa
     1681 cctgttgagg tgaaaacaga gaaattatat ccaattgaaa attatggttc tgcaatgaca
     1741 ccattttata gtgttctttc tacatgggtt ggtatattac ttatttcagc actattgtct
     1801 acatctgtac atggaaacta taaaccttat gaaatttact ttggaagagg ccttactttt
     1861 ttaagtatag ctcttgtcca aggttttata ataggaactg gagatatatt attgttagga
     1921 gtaacagcaa aacatccaat tttattgata gttctgctta tgtttactag tatggtattt
     1981 aactttatag tgtattcttt agtttctgta tttggaaatg taggaaaagc aattgcaata
     2041 gtactattag tcgttcagat tggttcttct ggtggtacat tccctataca agttacacct
     2101 acattcttcc aaagaataaa ccctataatg ccatttacct atgcaatagg tgctacaagg
     2161 gaagcagttg gtggtatata tgctccaaat ctaactaaag atatactagt tctattacta
     2221 tttatgattc tgtctatttg tttaaatgta cttctaaaag gacctataaa tagagttata
     2281 aaaccattta atgataaatt cggcgatagt aatttaactg gtcattaaaa aattaattct
     2341 gtctttaaat atattttttt aataatattc taagttcata ttaaaaggta gtagctaaaa
     2401 agctactacc tttttccaac ttaaaaacta atatttacta caaaaactgt catagaaaaa
     2461 aacgaaaaaa ttcgttcttt cctaaattta tcttataaat atttagatat attcctactt
     2521 aataagcaga gctatacata gtatttaatg tataacttac attatttaac tcatataatc
     2581 aattattcct gaatttaaat tatataaatg ctcaaaacct tcttcagcta acaaattaca
     2641 tgcagtaaca cttctatgac cacttctgca ataaactact actttcttat ctttataatc
     2701 gtaaatttca tctatattat tgattaactc ttgtaaagat atatttatag cattttctat
     2761 tttatattcc ttaaattcat ctttagttct tacatctaat aataaaacat ccttttcatt
     2821 tttcatcatt tctttaagtt gcttcttatt tatattctta tacatatgtc taaaattcct
     2881 ccattttatt tatatattac ctataccctt tattctttat acaacaacaa atattatttt
     2941 tatatttttc tctgttatat aaaaagctat acttataaaa gcttttttta ttgataataa
     3001 tctgttgatt aaaataatta agaatgttat gctaattata aattattaaa aagggaaaga
     3061 ggtaatgtaa tatgtttaag caaaggctct ctaaattatt atctagtaca ttagtattga
     3121 gtatgttatt tactgctgct ccaaatatta cttttgctga taatacaaaa gataattccg
     3181 aaaaatatca aagttctgat atagaattac atgattatag taaaaacgcg gaatcttaca
     3241 caaaaactaa agctcttgct aaagagaaaa tccaaacttt gctatcaaag tatggtgctg
     3301 taagtgctca atatgctttg attgataatg gcaaaattga gatttctggt aatggtggag
     3361 tttacagcaa acaggataac aaaaacttaa ataaggataa tatgtatagt atcgcatcta
     3421 tcagtaaaat gtttactaca acagctgtta tgaagttggt tgatgatgga aaattaaatt
     3481 tagatactcc tgttgtaaag tatatacctg aatttaaaat ggctgatgat agatacaagg
     3541 aaatcactcc aagaatgtta ttaaatcact catcagggtt gatgggaagt agttttaaaa
     3601 acactatttt attagcagac aatgattctt atggtcatga taatttctta aaagaattac
     3661 aaaaacaaag attaaaagct aaaccaggag ctttttctgt ttattgtaat gatggattta
     3721 ctttagctga aattttagta gaaagagtat ctggtatgtc atttactaat tttcttgata
     3781 aatacataaa taatccactt aacttacaaa atacaaaaac tccagaaaat agttttgata
     3841 gttcaaaact agcaaaagca tatgtacctt attgggaaga tgcagttcca caagataacc
     3901 ttaatgctat aggtgcaggt ggtttatatt caagtgcaga aaatctatgt acatttgcac
     3961 aaacatttat gaaaaattca aatggtattc tttctcctgc ttcagtaaaa gctatggaaa
     4021 acaaagaata tttaaatggt ctatggcctg aaggagaaga ctctatactt ggatatggtt
     4081 tagggtggga ttgtgtcaac acttatccat ttaatcagta taatttaaaa gctcttacaa
     4141 aaggtggaga ttctttatta ttccacagta atcttatagt tcttccagat gaaaatatgg
     4201 cagttgcagt tctttcttct ggtggaagta gtcaacttaa tgaaataatt ggacaagaaa
     4261 tcttactatc tgcattaaaa gaaaaaggta aaattaaaga aataaaacct gataaaacat
     4321 ttagtaagcc acaacaagtt aaaatgccta gctctttaaa agaaaatagt ggtctttatg
     4381 cttcttcaaa tatgataaaa gtagatgtta atgataatgg tactttaaca gtatcttctc
     4441 cttatataga aaatggacct gaagataaat acgtatacat aggtcaagat agatttgtat
     4501 ctgaaaaagg taatagttgt ttaaaatttg taaaagaaaa aaataatatc acatatttaa
     4561 atatgagctc ttatgatgat gtccctggat taggtcagac agcaagttta tactatgtag
     4621 ctcaaaaagt agatgacaac aatatatcaa atagtgtcaa agaagtgtgg aaaaaaagaa
     4681 gtggcaaagg ttactatcta gtggatgaaa aatatacttc tcaaagctat atgtttggct
     4741 ctgtaaaagc aagttttagt ctttctgatg aaactccagg ttatatagtt aacactaaaa
     4801 taatggatga gaataactca aatgctttta tagaaatacc tggtgtaata ggtcgtgatt
     4861 tatccgacat taaacttcat aaagaaaatg gtacagagta tttatctttt ggaacactaa
     4921 cgtatgttag tgaagattct ataactaatt tgccagcaga aaaatctttt acttgtgaac
     4981 tagaatccaa tggttatgca aaatggtata agattggtga cgatatcgca aataaaaaaa
     5041 tagaagttaa tcttcctcaa aattcagctt ttgctgttta tgatgataaa ggtgttcctg
     5101 taaactattc tttagttaca aagaacaata gagttagatt gccaaaagga ggcgtaattg
     5161 ttttcttagg aagtccaaat gctagatttg aggttacata tcaagatgaa gtaaatgcaa
     5221 gtgcacttac aggaactgat agatatgaaa cttctattaa gataagtcaa gcaggttggg
     5281 aaaatgctga aaatgcagtt ttaataaacg attcagcaat tgcagatgct ttggctgcaa
     5341 caccatttgc atacaagaaa aacgctccta tattattgac tggaagctct caaataaatg
     5401 aaaaaacttt ggctgaatta aagagattaa aagttaaaaa tgtatatgta gttggtggag
     5461 aagcttctat aaatgaaaaa tctttagata ctataaaatc aaacaatatc tctgtatcta
     5521 gaatttcagg aagtgataga tatcaaactt ctatgaacat tgctaaagaa ttgaacaata
     5581 ttagcaatat atcaaagatt tcagtagtta atggcgaaaa aggattagcc gatgcagtaa
     5641 gcattggagc tgtatcagct caaaatgata tgcctataat acttactaat gaaaatagta
     5701 atataactga aataaataac ttgtttaaaa acaaaaagat tgataaatct tatgtaatcg
     5761 gtggagaata tacagtttct aaaaatatag aaagtaaact tcaaaatcca cagagaatat
     5821 caggtagcac tagaaatgaa actaatgcaa aagtaataaa agaattttac aaggattcta
     5881 agatagacaa tttatatgtt gctaaaaatg gaatgaataa acaagatgat ttaatagacg
     5941 gattatctgt tggagttttg gctggaaaaa ctaaatctcc tgtaatgcta gtaggaaact
     6001 cactagatta taatcaaaaa gaactattta aaactatgag atttaaatct gtaacacaaa
     6061 ttggtggaaa tggaaatgaa aactcattta agcaaataaa agaaatagct taacataact
     6121 aaaaaaggct gtattcaagt aatattaatt gaatatagcc tttttttatt taagttataa
     6181 gttttatatg tgtaaaaatt aaatttaaat attaaatgat aagtttatta agatatagat
     6241 aagtctaaaa ctttgccttt tttaagttag tttctattta tcattcatac ttaacaattt
     6301 atctaaatat actgacttta attgtttact ggctaaaatc tttttagctg gttcaagctt
     6361 taatatagct gacagtatag ctcccatagc tctatgacta gccagagcat tattatcaaa
     6421 aattaaattt tgtaactgcc ctttttcaat tgccataaga actgctctat gtactgaatt
     6481 tgctggagtt attatttttt catctctctt taataacata atgctatttt tatggcaatt
     6541 tctaacacaa actccacaac ctagacatct atcctcatca atttttatat attctttacc
     6601 atcttcctta accttgctta tcgcatctat tggacatgca gttatacatt taccacactt
     6661 tacacaattt tcatggttta tatttggtat aaaacttgtt gtctgtactg gatgaagatt
     6721 tccaaatctt ttagctgcta ccattgcttc acagcaacat ccacagcaat tacatataaa
     6781 cgtaacccct ttcctaacat tttctccaca ttgaactaag ttatgttcat atgcttgatg
     6841 aagtagttct ttacattcta ttttatccac acgtcttgca aatttattat ttattaaaga
     6901 atttgctaca ttgtcaaacg tcatacatat atccatagga gcatcacaag cttttccaac
     6961 atgttgcatt ctatgtctac agtaacacat acttatacct atatgagttg attcatcaat
     7021 tatatgagta gccctttcat aatctaaaat actaacctca ttatcattag ttaaaacttc
     7081 ctcttgaacg tatactcttc caagcttcgt ttctgttgaa taaaataaat ctttgataaa
     7141 atcttcttct acattcatat attgatagta aagctctgcc aaaagttttt ggtctatatc
     7201 gtgtctcgtt ctcatcatgg caaactcaaa aaaaccagcc attggaggag gcattatata
     7261 tttcttccct ttattatctt ctatatctag tatcagagcc ttactagcaa gtttatccaa
     7321 tactctatat gcttcacttt cacttacact ccaaatttta gctgctgttt ttactctgaa
     7381 aggctttatt ggaagctgag caactaactt cgcttcttgt tctgtatata gtacatttag
     7441 tattttgtat aatgtatcag atggtggagc accttgtgga aacttgttta ttctttcctc
     7501 taaactttta tacatattct tcgcagttat atgagaaata gtaatcacct tctttttctt
     7561 ttgttttcca ataaaatcaa atattctttc ttattatata ttctcttact ttatataata
     7621 ccaattcttt ctaaatagtt aaatacctct agtaatttat tattagattt cttatataaa
     7681 taaaaaatca tttaagtgat ataataatat tttataccac ttaaatgatt ttaataat
//
LOCUS       DAMKMI010000164         7660 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMKMI010000164 DAMKMI010000000
VERSION     DAMKMI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.170"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            179..1582
                     /locus_tag="PC429_003317"
     CDS             179..1582
                     /locus_tag="PC429_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3848776.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1673..1913
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1956..2654
                     /locus_tag="PC429_003318"
     CDS             1956..2654
                     /locus_tag="PC429_003318"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF3848777.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2677..3678
                     /gene="trpS"
                     /locus_tag="PC429_003319"
     CDS             2677..3678
                     /gene="trpS"
                     /locus_tag="PC429_003319"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF3848778.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4330..5897)
                     /locus_tag="PC429_003320"
                     /pseudo
     CDS             complement(4330..5897)
                     /locus_tag="PC429_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(6233..6415)
                     /locus_tag="PC429_003321"
     CDS             complement(6233..6415)
                     /locus_tag="PC429_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848779.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
     gene            complement(6804..7550)
                     /gene="cdtR"
                     /locus_tag="PC429_003322"
     CDS             complement(6804..7550)
                     /gene="cdtR"
                     /locus_tag="PC429_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF3848780.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT         2737 a          913 c         1119 g         2891 t
ORIGIN      
        1 acttttaaat ttatcatttt taataagatt tgatttcaaa gtatttaagg tttattatta
       61 aaactgtttt tgaatatgca aatattttga aaaatatcag tttaataata atgaatctat
      121 tttattaatt ttgaaattaa atttaaaata attaatagag aggggaaatt aaattaggat
      181 ggaaaagaaa aaaataggcc tatggagttt ggtatttatg aatgtatcag ctttatatgg
      241 tataagatgg atagctaaat ctacagcatc aagctttggt ttaggattag gtgcaatacc
      301 tatgtggtta ttattctcat ttatatactt tgtaccagga gctttaattt gtgctgaatt
      361 agcttctaca tatcaaaaag gtgatggagg tctttacgat tgggttaagg aagcctttgg
      421 agaaaagtat ggatttttag tttcttggtt aaactggaca gctaaaatat tttggtattc
      481 ttcattttta actttcttag cagtaaatgt ttcttataca ataggaaatc cagcccttgc
      541 tgataataag atgtttgtac ttatattatc tttaataata ttctgggcat tatctcttat
      601 agcaactaaa ggaataagtt ttacaaaaat atttactaat acaggtgcat taggttcaac
      661 aataccaagt gctatactta taatattttc atttgtagca gtatttgtgt tgaaaaaaca
      721 tgatatagct tctacataca caatagcttc aatgactcca aaacttaata tggatgcatt
      781 tgttgctata tcagctataa tgtttggttt agcaggagca gagactgctg ctaacttcat
      841 aacagaaata gataagcctg aaaagaactt ccctaaggca atattaatat cagctggaat
      901 agtagcttca ttatatgtat taggttcaat tgcaataaca atgataatac caccagacca
      961 aattactgct tctaaaggtg tattagatgc actttctgct gtttgtgcgt ctttaggaat
     1021 tggttcttgg ttggttcaat taatagcttt tggtatagct ttttcagtat taggagcaat
     1081 agttctttac atagcatcac caataaaaat gttatttggt agtgttaaaa aaggaatatt
     1141 tccagatagt ttagtagagg taaatgaaca caagatacct tctaaagcag ttatacttca
     1201 agcaataata gttactataa tactattggt gactacatta atgccttctg ttgatgctat
     1261 atacaatgtt cttgtaacta tgacagcatt aactgcatta ttcccatatg tattgttata
     1321 tgcttcttat attaaactaa gaaaagagag accagatgaa attagacctt atactatggc
     1381 aaaatcaaca agtacttgcc taggtttagc aaaaatggtt ttagttgtaa ctgtagtggg
     1441 tatattatta tctgcagcac cagttatgcc tacatttgct gaaaatgtta tatatgagat
     1501 agagatgatt ggtggaggtc ttctagttat attatctggt ctatggttat ggaatagata
     1561 tgaaaagaaa acagctaaat aaaatgtatt ttaaaaattt cttttggata ttataatata
     1621 tagttgacaa tttatagtgt aaggaatatt ataaaataaa gaaattaata aattaaatcc
     1681 tgagataaga aatagtaaat aaatatattt ttgcacagag aattaaggat gctgagaact
     1741 taataaaaag tatttattga atgggtcttt gaggagtaaa atgaaatgta atttaatatt
     1801 acagagtatt tgatatcgtg tatgcacgtt atagcatata ttaagatata tatattcaat
     1861 tttgagtgta tataaattta ggtggtacca cggaaaatat atccgtccta ttgatttagg
     1921 acggattttt taatatagaa aatttaggag gaaaaatgaa cgtaatagga attataggtg
     1981 caatggatga agaagttagt attttagttg atttaatgga tattagagag actataaaaa
     2041 aagctagctt agaattttat aagggaattt tggaaggtaa gaatgtagtg ttagtaaagt
     2101 gtggcatagg taaggttaac tctgctcttt gtgcccagat actaataagc gaatttaagg
     2161 ttgatgctat tgttaataca ggtgttgcag gagctttaaa cgaaaaactt gatgttaatg
     2221 atatagtaat atcaactgat gctatacagt atgatgtaga cactacagca tttggagacc
     2281 ctaagggagt tataccaaga atgaagactt ctgtatttaa agcagatgaa agacttatag
     2341 atgctgctta caaatcttct gtggaagaag taaagactca taaagtatta aaaggaagag
     2401 tagttacagg agataaattt ataaattcta aagaattaaa agaagaatta gtaaatgatt
     2461 ttggtggata ttgtggagaa atggaaggtg gagctatagc tcatgtatgc tatttaaata
     2521 atactccatt tgtaataata agagcaatgt ctgataaggc agatggaagt gctgatgtta
     2581 cttatgatgt atttgtacat gatgctgcca ataactctaa agacatagtt ttaaatatgt
     2641 taaaatcaat ataaatatat aaggaggtac aaaaaaatga gtgaaaaaaa agttatattt
     2701 agtggtgcac agccatcagg caaaatgact ttaggaaatt atttaggagc tataaaaaac
     2761 tggactgaac ttcaagacaa ttatgattgt tattatagta tagttgattt gcatgcaata
     2821 acagtacctc aagaccctaa agttttaaga gccaatacta tagaattact tgctcaatat
     2881 atagcttgtg gattagaccc agaaaaaaat actatattta tacaatcaca tgtcaaagag
     2941 catgttgaac ttatgtgggt attgaatact atgacttata tgggtgaatt aagtagaatg
     3001 actcaattca aagataaatc acaaaagagt gaagcaaatc taaatgcagg tttattcaca
     3061 tatccagttc ttatggctgc tgacatattg ttgtatcaaa ctgacctagt tccagttgga
     3121 gatgaccaaa aacaacattt agaattagct cgtgatttag caaatagatt taataataga
     3181 ttttcaccta cttttgtagt tccagaagga tactacccaa aaggtggagc aagagtgatg
     3241 agtttgcaag agccaactaa aaaaatgagt aaatccgatt caaatgaaaa tgcatttata
     3301 ctattagcag atgattcaga ttctataaag agaaagataa aaagaagtgt tactgattct
     3361 ttaggtgttg taaaatacaa tgatgaacaa ccaggtatta aaaatctaat tgatatatat
     3421 tctaatttaa gtaaaaagag tgtagaagag attgtaaaca tgtatgaagg aaagggttat
     3481 ggcatattta aagaagatgt ggctgaagta gtatcagaag ctttaagacc tataagagaa
     3541 aaatatgttg accttcttaa taataaagat tatttagaaa aaatatatag tatgggagca
     3601 gaaaaagccg aaaaacaagc tagaaaaact cttagaaaag tttataaaaa agttggattt
     3661 atagagagaa gatactaatt attagctatc tttacttgat aaataaattt taaagttatt
     3721 aaaaaggtat atgatatgaa agagagagtc tcaaagtgaa cttttgttca tgaggcactc
     3781 ttttttgtat gaaataaaat atattttaat catttcaaca atgaaaaaga ggattatctc
     3841 tattttgaga cagcatcttt tgataataaa aaaagatgag cttttgctca tctcaaaggg
     3901 gttagattat tcttactttg gggaaagtag aataaaggta aaagttaact gatgtcaact
     3961 ttgtcttttt tgcatatcac tactgagcat aaaaaaatca gaaaaccata gccttctgaa
     4021 acaacttcta acatacattg tattttatac ccaatatgta atttatgagt taattttaac
     4081 tcttataatg aaaattgtca ataatatatc aaaaaaatat tttttaaatt tatataatta
     4141 aatgaattta tgtttatata taggtgatat gatacagata attatctttt taatacaata
     4201 tagttctaaa aaagaatata ttagaagaag ataatattta aataaaaaat accttataaa
     4261 gttaattttt taatacttta taaggtaaat acatattgta ttgtggggat aaatttaaat
     4321 catttgatac taatcaacac taagaaccaa taactctcta tcatcttgag taattgtata
     4381 tattcttaat ttcttgtatg tcataatatt ttctccacct gtaaaataac tcctaagatt
     4441 aacacaatca gttttaggtt gatttgtatc tcctaaataa ttacgcaatt ctttatcttt
     4501 agtttcaata tctttaaatc caaaatattg taaagtagct tcaactctat atttttggac
     4561 ataatcgaga gcttctttat tagtttatgt tggtgtaaaa tacataccat ttatataagc
     4621 ctttattgta cttggattaa atttaaaatc tgcagaatat attttatgtg taactattat
     4681 ttcttgaaca gttggaattt taacttctgg ttcatcaagt atttcaggag tactatttag
     4741 ctctgtaata gataagttat ctaagtatat tgtatcacta tcaattaatg ctatatttat
     4801 attagaagaa tctttttcag tagtttcaaa ttcataacta aattttgtat atccttgttc
     4861 tagtaccaaa taatccgttt tttcttcttt tacttttata tttacaatta ttgaattata
     4921 tgttaaagga ttttttgaat tttactaaaa acataacgtt tataaggttt tagttcagac
     4981 ataggtattt taatcttcgt ctcaccattt aatctatttg ctgagccttt taaaccatct
     5041 tgatttgtag tattaacatt actccatata ctagggtaat tattgtaatc atcaaaatta
     5101 gtaaagtaag ttggtgtttt tataagtata ttcattcctc tttcaagttt aacattacat
     5161 atttgtttat cagacaaagt ttttaaactg tctttaatct tattgcctat attatcatca
     5221 aatatgagtt caacacaact ttcatctatt ggtatattat taaaatataa caaaccatct
     5281 tttttagtag tgccaaaagt tttttcaatt gcttctccaa ttgtaagttc aggtgtttta
     5341 tcttctggga cctgtaaact tttagcagta attcttcttt tgtaagattc attttctgta
     5401 tctaatataa tagatacaga aatactgtca atttgaccta tatcgtctta ccaactattt
     5461 cattctgtta ctatttgtcc agaaccattt tttgtaccaa aatttccact tacttgtgtt
     5521 gtttctaatt taatttgctt tccatcatct aattttttta attaattata atttatagga
     5581 atcagtctag aactaaattg atacattgtg ttaagagcca taggtgaaaa tccttttttg
     5641 agataagtat ttccaggaga tagattattg ccaatttgat tttcttgtgc tttgatagtt
     5701 gataatgcat ctccatctaa cactaaattt gttgttggtg tcactttgta cataggtgca
     5761 gtacctgtgt tgtagtatct aatatttgca tttatatatg cagattctcc tttgtttata
     5821 cttaatccag tatttcatga ttatccattg ctattttgaa cagcagttga attatctgtt
     5881 gtatggtaat aatttgtctt gtatagtttc tttaaactca ttaaattttt ctaatgaaat
     5941 ttcgttttga ttttttgttc ttattacttt attaaatgca aatttttctg gagattcaaa
     6001 ataatataca tacataggtt tatctatttt attttttgat atagcatttc gaagttctct
     6061 atattctttt tctcaagaat ttgcttctat ttgataatca taaaaataat ttcttgtctg
     6121 agaatatttg cttatctcta tagaatcttt tttatatgat cctaatactt ctttttcaga
     6181 tttttcaagt ttttgatctt ttctttctca ttctttagcc ttttctttat ctttaaaaaa
     6241 atccttctga tctttctata ggagccttgt aagtagtatt acaaactttt ccactatagc
     6301 tttgtaaatc ttgtgcataa atgttattag gtaacaaact acctaaagtt agatataata
     6361 tcaacaatat agatatacaa ttactaatac ttttatgttt cctaaatttt ttcatttatt
     6421 ctccctacaa atattagttt agttaacttt tgaattttaa tttatttaga ttatataatc
     6481 taaataagaa ttttttatat aaatttcata aaatatgtag gtatatatca taaaaatatg
     6541 tatttatatg atatgtcagt acaaaataac aactgaatat tttaaagaga ctatttcgta
     6601 cttttatgat ttaatgagct atcttatcga atcataatct aggaacaatt gttataatat
     6661 ctaatagtag ttatatatga tatagtcagt atgtgttttt tcacaatatg attattatat
     6721 agaggattct tagggattac tctttttgta taaaaaagag atggttcaaa tttttatctt
     6781 gagacatctc ctttttctat ttattatgtt ttaataatat tctttaaaat atttctatat
     6841 gtattagata tacgacatat atggccatta ctcattttaa tattattttt ctttaggtcg
     6901 agagaaacaa tatgatattt attaactaca aaagatttat gtacacggat aaagcatgca
     6961 tctaaatctg gtaaagactt taaactttta tagaattcaa atattctaga agtagtcact
     7021 aattttattt tatgtggaat agtgcttgtt tcaaaatata taacttcatc aaatgaaatt
     7081 ttccatagtg agaaatctga atagatagta aaaaaattag agcgataatt tttttcttta
     7141 atgtatcttt tgtgagcaat tttaatacaa gcttttattt tactttcagc aatttccata
     7201 tcatgtttta gtataaagtc cataacttct aacctataat cgaagatccc taaaatcatt
     7261 tctatatgat ttgtaagaaa tatgattgga ctaatataat ctgattctct aattttttgt
     7321 gctatccata atccatcaca taattcatta cttatcttat attttgcgtc tacaactata
     7381 aaatatattc ttactttatt tttttcagta agctctttta ataacaaatt tgcattatga
     7441 tataatctta caacatcatt atcaaaacct tcttttatta aaatattatt tattttttct
     7501 tttagtttca ttccaaaaca aacatcatta tcaaagatta atatatccac taaataccct
     7561 cctataaaaa attcaaaagt aaaaaaacaa aagtatttta tagaaaatac ataaaataac
     7621 actaggagat aaataagatt aaggatagtt agaattaagg
//
LOCUS       DAMKMI010000165         7643 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMKMI010000165 DAMKMI010000000
VERSION     DAMKMI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.171"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            953..2311
                     /locus_tag="PC429_003323"
     CDS             953..2311
                     /locus_tag="PC429_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF3848781.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2418..3212
                     /locus_tag="PC429_003324"
     CDS             2418..3212
                     /locus_tag="PC429_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF3848782.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3205..5511
                     /locus_tag="PC429_003325"
     CDS             3205..5511
                     /locus_tag="PC429_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF3848783.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5650..6927
                     /locus_tag="PC429_003326"
     CDS             5650..6927
                     /locus_tag="PC429_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3848784.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7110..>7643
                     /gene="yqeC"
                     /locus_tag="PC429_003327"
     CDS             7110..>7643
                     /gene="yqeC"
                     /locus_tag="PC429_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF3848785.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSLINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLK"
BASE COUNT         2987 a          839 c         1434 g         2383 t
ORIGIN      
        1 cttgtatatt ttatgttgca gatacaaata tagtaaagga gagtacattg gtacaactga
       61 gatacatagc ttattaagtt tttctgtctt atgagaatta tattataaaa tattcataaa
      121 tattttgagt ttaaattaaa taattatttt atgcttagat taattgatat ttaattaatt
      181 taataacaaa aacagtgaaa gagtaaataa tattatttac agggggtaaa gcaaagtatg
      241 ttaaataaat tgtacttttg gccccgtaca tagagaaatc tatgtaggac acaactttaa
      301 aaccagtaaa tcctaaagcc ttgcaactac aataaaagat agaaagaaac ttttatgaat
      361 gtggtgaaaa ccagaaacaa tagtgaggat ggtatatggt gagagcctaa gtacttaaaa
      421 atggacattt ggtcgccaag cctagaaata ggaaggtcaa acgactatct cgagagagag
      481 tacactataa gcgattgata gtggaaacgg gttgctctta gcaggtaatg ctgaagatga
      541 agaaatagtc tacgctcata tgaaagtatg aggtcactgg atttgaagtg acatctataa
      601 agtagcgttt ataggtaaat gtttaaaact tgaaacatgt aaatacttat gaaaacttat
      661 aacttataat gattaagatt gaaagattta aattgaatta aatttactat aaaaaattaa
      721 aaattcatag ctataatact cttatatttg taaagcatgg tatataatgt atacaaaatt
      781 aaagaatacg tttatttgtt aataattaaa tttattgcat ctgtttttta agactaaaaa
      841 cttaaatata tgttggagta tagttgatat ttatgaacat cattaattta atatattaga
      901 tttttaagta tttatataat tcaataagtt ttatgtaacg ggagttttta ttatgaaagc
      961 aaaaagtaca tcaaaatttg aattagacgg aataccacca ctaaaggaag caataccttt
     1021 agggttacaa cacttaatgg caatggtaat aggtagttgt ttaccagcga tattgattgc
     1081 aaatgcagct ggacttaatc ataagatgtc aacattattg attcaagccg cattagtttt
     1141 tgcaggatta tcaactttat tacaattata tccaatacct ttgtttaaag gttttaaatt
     1201 aggaggagga ataccagtta taatgggagc tacagctatg tttataggag ctggggtatc
     1261 tgttgcaagt aagtatggac taccagtatt atttggctct caaataatag ctggtatagt
     1321 aataattata attggttttg gtcttaaaaa aatacgattt atatttactc cagctgtatc
     1381 aggaacaata gttgcatgta tgggtcttgg actttttcca atagctataa aaaacttagc
     1441 tgggggtatg ggcaatgaga cttttgggaa tccaattaat tttatagttg gaatatctgt
     1501 atctcttatg atattaggat taaataagta tggaaaagga ttatttaaag atgcatcaat
     1561 attagttagc ataatttttg gatacatttt atcattaatt ttgggtatag taaatttttc
     1621 ttcaattcag gagtttactt tggttgcact tccaaaacca cttgcttttg ggctagatat
     1681 aagacttgaa gttgttgtaa tgttttcaat aatatattta gttgagatag ctgatataat
     1741 gggtgcttgt acactttctg cggtaggagg gcttaataga caagtaacag atgaagaact
     1801 ttcttctgct gttttaggaa atggagtact ctctacaata ggtgcattat tttctgcaac
     1861 tccaatgggg atgtttggac aaaattctgc aatcgtttcc aatacaaagg ttgtaagtaa
     1921 atttgtacta gctattggag gaattggatt atttttggca ggaatatcgc ctttattagc
     1981 taatttaata agaacaattc ctccctgtgt ggttggagga gcaacactcg ttatattttc
     2041 aactcttaca acatctggtt taagacttgt tagtatggat ggatttaatc aagaaaatag
     2101 catgatatta ggtctttcta tggcttctgg tattggattc atggtagctc ctcaagttct
     2161 tgaaaaattt ccaaaattta tagagacatt acttgcagat tcaagtgtag tttcaggagc
     2221 aatggtagcc ataataattc aagctttata tatggtaaaa tttcctggaa ataaaagtga
     2281 taaggttgag gataaaaata aaagtttata atgtaaaggt ttaatatatg tgaattaaat
     2341 atactacaat tataaatata aataaaagat ttattaaaaa cttttgtgtt ttaataagtt
     2401 aatgatgggg ggataagatg gttactataa aggaatattc agttccacag agtttagaag
     2461 aagcatataa aattttaatt tctaaaaaaa ataatgttat tttaggtgga tgtggattta
     2521 taaaattaag taataaaaat ataggaacag cgatagattt aaaagatata aatctaaatt
     2581 atataaaaga ggataaaaaa aatattttga taggtgcaga tacttcactt agaagtttag
     2641 aaattgataa aactataaaa aattactgta gtggaatact ttcaagcgct gtttctaata
     2701 tagttggtgt tcaatttaga caaggggcta gaattggtgc aagtgtattt tcaaagtatg
     2761 gcttttctga cttaatacca gcgcttttgg ttgttggagc gaaggtgaga ttatacaata
     2821 aaggaatact agagttaagt gagtttttgg atagtgaatt aagtagggat atattggtcg
     2881 aagtaatact accaaaaaaa gatgcaatag ctgtttttga ctcaataaga aaatgcacag
     2941 gagatttttc tgtattaaat tcagcaatgc taaaagaaaa tgatacttac aaaatagcaa
     3001 ttggggcaag acctcaaaaa gctagattag cacttgaagc gagtaatata ttaaataaag
     3061 aaaaagatat agataaggca tctattgttg ctagtaagga acttacttat ggaagtaata
     3121 tgagagctag taaagagtac agaaaggata tggcaagtgc attagttaaa aggatgtata
     3181 atgctattga ggagggaatg tataatgatt aataagggga taagaaagat agatagtaca
     3241 gcaattgtca caggaaaacc tctatatact gaagacctca ttatgcataa agatgttttg
     3301 acattaaaac tacttagaag tccacatgct catgctaaga taaagaatat agatacatca
     3361 aaagccgaaa aaattccagg ggttgtgggt atttatacgt ataaagatgt accacaggtc
     3421 agatattgtg tgccaggtca aacagaacca gaagcatcac cttatgatag agttttactt
     3481 gaagatacag ttagatttat tggagatgaa gtagctttag tggctgctga agatgagaaa
     3541 acggcactta aagccttaaa aatgataaag gtagattatg aggttttaaa gcctgtgttt
     3601 gatgcaagag aagcagaagg aaatgaagtt gttatacatc cagaaaaaga catagtagag
     3661 ccatatcctt ttggattgga tgcaaaaaat aatatagtat caaaagaatt ttttgaattt
     3721 ggagatgttg aagctaagct taaagaaagt gattttgtgg ttgaatcttc atacaaaacc
     3781 caaagtcaat cgcattgcat gatggaaact cataggtcat attcatataa agatataaat
     3841 gatagaattg ttgtagtttc atcattacaa tcaccacaca atgtaaagag aatacttaga
     3901 aaagtattgg gtataccatc ttcaaaaata agagtaatta aacctagaat aggtggtgga
     3961 tttggaggaa agaatatagc agcaacagat atatttactg catttgttac tatgaaaact
     4021 ggtagaccag caaaatttgt atttacaaga agtgaaactt ttgtaggttc taatagtaga
     4081 catgaaatgt tttttgatgt taaagttggt gcagataaag aaggaaattt aaaagcaata
     4141 gagctaaagt cattaaacaa tacaggtgct tatggtggga atggtgtctc tgtttcttcc
     4201 gaatctggac acaatacctt accaatatat aatgatattg atgctgttag atttaatgct
     4261 agaacagtat atactaatag gttaccagca ggagcactta gagggtatgg tgctacacag
     4321 ggaatatttg caatagatag tgctattgat gaattggcac ataagattgg aatggatcca
     4381 cttgaattta gaattaaaaa tataatagat aggtatacca atggaaaagc agctgaagac
     4441 gaaatattaa gttgtgagct gagacaatgt atagaaaagg gcaagaaatt aattaattgg
     4501 gatgataaat atccatgtaa agatatagga aacaacaaag taagagcagt aggtatggca
     4561 gtagcaaatc atggttctgg tataccgcgt gtagatatgg caacagtcac tataagaatg
     4621 gatgaagatg gcacatataa actattatct ggtcttgctg acttaggtca aggggctgat
     4681 acaattcaag ctcaaattgc tgcggaagct ttaaatacta ctgtagatag aatttctatt
     4741 tatacaggtg attcagactt atgtccctat gatacaggag catttgcttc ttgtacgaca
     4801 acggttacag gaaatgcaac aataaagaca gcaaataaat taaaagatat attactttca
     4861 gttgcagagt caaagctagg atttaaaaaa gaagatttag agttgggaaa agacagggtt
     4921 tttgttaaaa acgataatag caagtttgtt ctattagagg aattgggtag agaatctgtt
     4981 ggaggaatag gatttgctag tgatgatgta ccagtagttt tggaagcaac agaatctttt
     5041 ggcatgttga aaaaaataaa accatttata gcaggatttg cagagataga attagataaa
     5101 aatacaggcg aatataaagt cataaatttt gcatgtgtac ctgattgtgg aacagttata
     5161 aatccaactt tagctagaat acaagtagaa ggtggtgtga tgatgggtat agggtttgca
     5221 atgtatgaag accctatatt tgataaagat ggtagattat taacagatag tttcctacag
     5281 tataaagttc ctacaaaaaa agaagttgga aatatattgg tagattttgc tgagaatttc
     5341 gacccaggag gtccttatgg tgctaaatca ttaggagaaa tagttataca tacaccagca
     5401 ccagctatag caaatgcaat atttaatgct acaggagttc gtttaagaga gcttcctatg
     5461 acttttgaaa aagtttatat gggtatgaaa aataataata aacaattata ataaataata
     5521 agatgtataa aagcaagtaa atttaaatta ttttgtaaaa taaagtaatt aaatggacat
     5581 tatatatggt gttttgtaaa aataccgtat ataatgtcta gtagagtata ttctaaagga
     5641 ggtgagttca tgtcaaacag aataaaaaat atacactatg gaactcagag acaacaatta
     5701 aaaaacttaa ctaaaaaaga atatcaagct ctacgagaaa tgtgcttctt agctaagaac
     5761 ttatataatg ttggtacata taatgtaaga cagcattatt tcaatcaagg agaacatctt
     5821 aactatgaag ctaattatca tgtatgcaaa gaaaatgaga actataagct tctcaatagt
     5881 aatatagcgc aacaaatatt aaaagaagta gacagttcgt ttaggtcatt ttttggatta
     5941 attaaattag ccaaagaagg aaaatatgac tttagaggga taaaatcacc taaatattta
     6001 gataaagaag gattttttag cataataata ggtcaaataa gaatcaaaga agatgggatt
     6061 ttaaatgtac ctatgtctcc agcatttaaa aaactatatg ggaaagtaag tataaaagtt
     6121 ccacaaaata tactagagaa aaaaattaaa gaaataagaa taatacctaa acataaagct
     6181 aggttctttg aaattcagta ttgttatgaa ataccaaaat cagaggaagt ttcaaatcaa
     6241 aaaaatgcac tagctataga cttgggttta gaaaatttat gtacatgtgt taccaattta
     6301 ggtgatagtt ttatcataga tggaaaaagg ctaaaatctg tgaatcagtg ggcaaacaaa
     6361 cagaatgata aaatagaagc cttaaaatta aaaaagaata taactgcaat aacaagaaaa
     6421 caatttaaag tttggaataa gagaaataat caagttaaag actatgtaaa taaaacctgt
     6481 ttttatataa ttgattattg tgtaaaaaat aatattggaa atttaattgt aggatataat
     6541 gctaagttag aaaaaagtga taatatggag agaaaaatat atagaaattt tataagaatc
     6601 ccatttggag agataaaagg aaaattagaa tatctgtgta aaataaaaaa tatcaatttt
     6661 attaggcaag aagaaagcta tactagtaaa tcagattttt ttgctgatga ttttctacct
     6721 gatatcaatg tggacaatgt taaaaaatat gactttaaag gggaaagaat aacgcgtgga
     6781 cagtacaaat ccagtgttgg aatcattata aatgcagatg taaatgcgag tcttaatata
     6841 ctaaaaaaat cacaagtagt taatttatct aatttacaga ataatcctca aatattaaag
     6901 caaccacaga gagtaagaat atcttaatta tatttaaaag ataaactgat agcgtaagct
     6961 tacagcttag cggcgaattg gatccgcctt gttgtttaca acagaatcta tttactttcg
     7021 agtttatgga atttcaaaaa tcacttttac taaaaaggtc taactataat aattaagaga
     7081 ttagaaaact ggttataaga ggtgaaagtg tggaactatc aaatataata gaaaagaatg
     7141 aaataataac tgtagttgga gcaggaggaa aaacttcgct tataaactat tttgcaaatt
     7201 tttatagaga taaattgaaa gttctcctta caacaacaac taaaatatat gtaccaaacg
     7261 attatgacaa tataattatt acaatagatg gaactgttat tccatctatt tgtcatggaa
     7321 taacagtctg tggcagttat ataaataatg aaaataaatt agtaagtatt gattcctcta
     7381 ttttggatga aattgtagac caatttgatt tagttttaat agagggagat ggctcaaaga
     7441 gaaaaaaact taaaggttgg aatgctaaag aaccagtagt atatcataaa actacaaaaa
     7501 ccatagggat attagatata acatcatttg gaatgaatat taatgaagag aatatacata
     7561 gagtagaaat atttaaaaaa atagcaaatt tagatacaag tagtattaat agtagttcta
     7621 cagtaagtat tgaaaactta aaa
//
LOCUS       DAMKMI010000166         7636 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMKMI010000166 DAMKMI010000000
VERSION     DAMKMI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.172"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            139..1440
                     /locus_tag="PC429_003328"
     CDS             139..1440
                     /locus_tag="PC429_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3848786.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1516..1791
                     /locus_tag="PC429_003329"
     CDS             1516..1791
                     /locus_tag="PC429_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3848787.1"
                     /translation="MLELRKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3234..3464
                     /locus_tag="PC429_003330"
     CDS             3234..3464
                     /locus_tag="PC429_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3848788.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3457..4038
                     /locus_tag="PC429_003331"
     CDS             3457..4038
                     /locus_tag="PC429_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3848789.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
     gene            complement(4613..5290)
                     /locus_tag="PC429_003332"
     CDS             complement(4613..5290)
                     /locus_tag="PC429_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF3848790.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5479..6843)
                     /locus_tag="PC429_003333"
     CDS             complement(5479..6843)
                     /locus_tag="PC429_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF3848791.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            6971..7492
                     /locus_tag="PC429_003334"
     CDS             6971..7492
                     /locus_tag="PC429_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF3848792.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2971 a          867 c         1324 g         2474 t
ORIGIN      
        1 attaataatt tgtagtatat catgacgaat aatacagtca taacaaatag tgagtcataa
       61 caaataatgt atttataatt gataatgtat ttaaaaatca aaatataaaa acttataagg
      121 aaagagagat taatttatat gggtaaatca aaagatgttg aaatagagaa gtctatttta
      181 ggcacaattc ttttagacaa taaattaagc tataagctag atgaattaaa tgaaaacatg
      241 tttatgaatg atatatgtct tgagatattt aaaattatga aagaacttaa aaaagaaaac
      301 atagtgattg atgtggccac tgtgaaatca aagatagata gaaaatctct agcaataaaa
      361 acgagtgatg tgactaatct aattacttgg ggtcagaatt ttggtcttga tggtcatatt
      421 aaaattttaa aagaaaattt agctagaaga agcataaatc aaaattgcca aaacctttta
      481 cacagcctaa accttggaga aaatatcgat acttgtattt acaaatttga aagtaatata
      541 aaggagattt tggacaagga tacttatgaa aatgatgatg taaatagtat agcagggaag
      601 gtgcttgatt ttttagaaaa caaaaaagat attggattta aatttggtat aaaattactt
      661 gataccacta taggaggtct ttttaaagga gagctaacca caatagcagc taaatcgggg
      721 gttgggaaga ctgctttggc acttcaaata atgttaaatt catttaagca aggtaaaaaa
      781 actttattta taagtagaga aatgaccagt gagcaagtat ttatgagaaa tatatgcaga
      841 gtaactggtg taagtacaag gaatatgaaa tctaaagaaa tagatgaaaa tgattggaaa
      901 cttattgtaa atgctatagg tgatttaagc gaaaataact taatttatat caatgataag
      961 atagatacaa ttagtgcaat tagaaagagg ataaggcagg taaagccaga tttattaata
     1021 gtggattatg tgcaactcct tacatctcag aaaagcatgg ataagagaga aagggaggta
     1081 gctacttttt cgagagaact taaaaatatg actttagatt ttaatatacc tgtaatccaa
     1141 ttaagtcagt taaatgatga aatgaaagat tcaagacctt atggagatag acctatgagg
     1201 gatagcaaag ccatctacca cgatagcaac aatgtggttt atatacatca attaaagggg
     1261 agtgattatg aagaagcagt gagagatatt ggagaaagtg aggaggcagt tagagcatca
     1321 gaatacaggg gaattaagat ggttgattta atagttgcga agtgtagaga tggacagact
     1381 aggcataagc atttttgtta ctttggggat aagttacatt ttcaagaact aaattattag
     1441 gtcaataaag tagtaataac aagcttatta aaagtcggga aatagcaaat agattataaa
     1501 atatgaggtg aaaatatgtt ggaattaaga aaaattatag aattacttga agaacacaag
     1561 atagaagtaa gtattccaaa ggagttacat aagtatagtt tattggaata ccttgaattg
     1621 tgtcaacaaa gtgggggaac atatttttat attaaaacac tggaaggggc tttaaaagag
     1681 gctagaaatg agcttatgat taaattgttt aatgggaaaa attatatgga attagcgagg
     1741 atgtttgaaa tgagtgttat taatgtcagg catattgtta gaaaaaaata gatatttttg
     1801 gagaatacaa gtgtgatata cttctgaaat agaagttatt atagttgaaa taaaattgtg
     1861 gttaataaat taaagttgtg tataagttta agattataga ggagtataag tgtgattaac
     1921 aacatgattt tgtgaataaa attgaggata tatatgattt aaatgtgtga ttaataaggt
     1981 aaggttgtgt ataagattat gagtatttaa aaggaggggc tataattaat agtgtagagt
     2041 tgtgtgtttg cttaagatta tataataatt taatagatta aactatacta ataaggtaaa
     2101 atcgtgtgtt tagttaaagc ttattataaa atcttgatat gtgattaatg atgcaatatg
     2161 tgaataaagt taagattata atagatttaa cctgtggcta atagaataaa attgtgaata
     2221 aagttaagat tataataggt ttaacctgtg attaatagag taagagtgta aatagagata
     2281 aacttcaata tgagcttaac ctatgattaa tagagtaaga gtgtaaatag agataaactt
     2341 caatatgagc ttaacctatg attaatatag taagagtgta aatagagata aacttcaata
     2401 tgggtttaac ttgtgattaa tagagtaggg tgtaactaaa gataaacttc aatatgagtt
     2461 taacttgtga ttaatagagt aaggtgcaac taaagataaa cttcaatatg agcttaacct
     2521 atgattaata gagtaagaat gtaaatagag ataaacttca atatgggctt aacttgtggt
     2581 taatagagta agagtgtaac taaagataaa cttcaatatg ggtttaactt gtggttaata
     2641 gagtaagaat gtaaatagag ataaacttca atatgagctt aacttgtgat taatagagta
     2701 agagtgtaaa tagagataaa cttcaatatg ggattaactt gtgattaata gagtaagagt
     2761 gtaactaaag ataaacttca atatgagctt aacctatgat taatagagta agaatgtaaa
     2821 tagagataaa cttcaatatg ggtttaacct gtggttaata gagtagggtg taactaaaga
     2881 taaacttcaa tatgagttta acttgtggct aatagaataa gattgtgaat aaagttatag
     2941 ttttagatgg gtattaacct gttactaata gaatatgatt aggtgtaaaa ttatagagct
     3001 gtataagaaa atattataga tatattaaat attagataat aaatatttat atagatacac
     3061 aaaagaaaag gcgatatgaa gctaaactta atgacttcat atcgtttttt atttgaaact
     3121 cctttgtatt tagagtgtga tttttaatat tgaggtttta tgtgattaag tcacagataa
     3181 aaagtaagta tcttgatata attcttatag attaataata tagggaggtt attatgtcta
     3241 ataaaaaaaa gaagtatgca aatattttag caaatgagtg cgttgcttgt ggtagctgta
     3301 taaaagcatg tccaagaagt gctatctcag ttccatgtgg cattagtgca aaaattaata
     3361 gagatttatg tgttggatgt gggatttgtg aaaagatatg tccagcatct gtaattgaga
     3421 ttataacaat attaaaagag gaagaaggta agtgtcatga ataataaaaa acattggtat
     3481 gactatttat ggattttttc ttcaatatat ttgatattgg gatttgtaaa tattattttc
     3541 gcttggattg ggctaatttg tttctttgtt ccactagcta tttctattgt taagggaaat
     3601 aaagcttact gtaataagta ttgtggaaga gggcaactgt ttaatttgct tggaaataaa
     3661 ttgaagctgt caagaaatag agatattcct aaatttatta gaagtaagtg gtttagatat
     3721 gggtttttga cattttttat ggtaatgttt gccaatatgc ttttttcaac ttatttagtg
     3781 tttgaaggaa gtagaaattt taaagaagta gtgactttgt tgtggactat aaaaatgcct
     3841 tggcaatggg cgtatcatgg aactttagtt tcaccttggg tggtacagtt tgcatttggt
     3901 ttttatagtg ttatgcttac atcaactgtt cttggtttga ttactatgat attgtttaaa
     3961 cctcgttcat ggtgtgttta ttgccctatg ggtactatga ctcagatgat atgtaaggca
     4021 aagagtaagt ctatatagaa aggctttatg tgatatttac agttttgtta gagatgagta
     4081 gtaggtttga atatatatga caaatatatg agtatagggg catagattta gtttgtgtaa
     4141 ggtaaactga tttatgcttc ttttttatgt cttagattat tggctaagtg gaaacaatga
     4201 atttagatat taagaattat tcagttattt gaagaaaata gaataataat taacattata
     4261 tgaatggata ttcattacat ataagccagt taatagcaag atttatttaa tatgaaatag
     4321 gtcaagtatt taaactaaat aaggtcatga caattaatat aagtatagat ataaaaagat
     4381 tagaagttat aacttataaa aggtattcta tataaatagt atgtggaaaa tagtcaaatt
     4441 aacttattag aaacaaaaaa tatacataaa aaaataaatg taaatatcaa ataagatact
     4501 atataaaaat agatataagt atctttttta ttattgaaat tatgaaaata tatttgattt
     4561 cacacaaaaa agagtgcctc atgaactaaa aagttcattt tgagacacac ttttacagtg
     4621 tcaaagaacg aagtttatcc ttatccaata cttttatacc accacgagat aaagacacaa
     4681 taccttctct agcaaaatat ttcaacattc tcgatacaac ttctctggca ctacccatat
     4741 acttagcaat ttgctcatga gtcatattta tagtgctact gccattttta gctatctcat
     4801 caattaaaaa agatgccaaa cgcttatcaa aactcataaa aagaatttgt tgcatagccc
     4861 acataacatc agaaaaacgt tcggtagcta atttatatga aaataactct gcatgaatat
     4921 tttctgtaga taattttgca aaaacagagg aactaatctg tataatatcg cagtccgttt
     4981 cagcatctac atagacatca aaagttattg tttttagaat acaggaagct gataaaatac
     5041 atacatctcc atcatcaagt cggaacagtg taacctctcg accttcatct gaaagtatgt
     5101 atgtacgtac tgttccagac tttataatca aaataccaat acagtcacta tcaccacaat
     5161 gtactggatt cccttttttg taatgcgagg tatttgctga agaaattaat aattcttttt
     5221 gtaagtcagt aagtttatcc caaaaaggca agctatgtgt aagtatatcg atttcatttt
     5281 gtgaaatcat acaatatctc ctttcttaaa ttgttataat cccatgatac aagtgaatag
     5341 ataaattgac aagtagtttg ttaataggat tatttagtca gatagtaata gtatgattta
     5401 tgtagaaaaa atatgataat atatattctt gctattaatg agaaggtaat atgttttgat
     5461 gatagtaggt ttgtataact aatgcatacc gtttattttt aaaagattaa gttccttatg
     5521 taaccttata gacataaaac cagcaattat agcagccatc aaatcagcta ttggttgtgc
     5581 atataagata ccatttaacc catagaatac aggaataatc aaaattatag ggataaaaca
     5641 gacaccttgt ctacacatac ctaaaaatcc accttccttt gcttttccta gtgcgaggaa
     5701 taatgtagaa tacactgtat agaatccaaa tagcataaat gaaaacccat ttgccctaag
     5761 agctttacta ccattttcta ttaatagaat gtcaccactt gtaaattgtg aaattataga
     5821 ttttgggaag agtatcaata taagaccaag cactacacaa aatattgtag tccataatat
     5881 ggaagttttt attgctatat gtaatctatc gtagttcttt gaaccaaaat tataaccagc
     5941 aattggttgg aaacctttta taaaaccaaa tacaacaaga ctacctaaag acgttattcg
     6001 tgttacagca cccattccag ctatcacaga atcaccatac tttgtagcct ccatattagt
     6061 taatgtaatg gcaagactag tcaataattg aaatattaag gtaggtatgc caatttttag
     6121 gatttctgaa aaaatttctt ttgaaaaaca gcattcgtta attctaaaac taaatatact
     6181 tttcttgctt aaaatatatc ctacataaac aagtgttgaa gcaatctgtg agatagctgt
     6241 tgcaatagaa gcacccctta cccccagacc aaaagtataa atgaatattg ggtctaaaat
     6301 catattaagc aaagcaccta ataacatagc acacatagtt gttttagcag ctccttcact
     6361 tgtgacaata ttagtcatag tgacattgaa tatattaaaa atagatgaca cgacataaat
     6421 gctagcataa gatattgcat aaggcataat actttttgta gctcctaaca attttaaaat
     6481 tggattgaga aaaatcataa tcacaataat aactaatgta cccactataa tactactgta
     6541 aatagatgta cttaccactt ttcctgcttt ttcattatca ccatggccta aaagtctggc
     6601 aatatatgaa gcagctccat ttccaaacaa taatcctaat cccacaacaa gctgacctaa
     6661 tggaaatgca acggatatag ctcccatttg acttgttcca agtccaccca caaagtatgc
     6721 atcaaccaaa ttgtataatg cagttatcat catacctatc atggtaggta atcccataga
     6781 taacaatgct tttgagattg gggcagaccc taataattca attcttttgt tactttcttt
     6841 catacttaac actcctcttc tattgacatt aagtctaaat tataatacta taaattatag
     6901 taagtatgga aaatagtact ataatttata gtatttgtca atagtaagaa aactaaagga
     6961 gatgattttt atggctacaa tagatttaat tgtattggga atagtaaagc aacaacccaa
     7021 gagtgcttat gatattcaaa aggcagtgga atacaggaat atatcacgtt gggttaaaat
     7081 aagtacacca tcaatttata aaaaagtaat tcaattagaa caaaagggat atattgaaag
     7141 taaaactgtg aagaatggaa aaatgccaga aaaagcagtt tattcattaa ctgaattagg
     7201 tgagacacat tttttagatt taatgtttga tacatctagc aaaccaataa atattctttt
     7261 agattttaat gctgttatag taaatttaaa tagccttaca ccagaaaaac agaaattatg
     7321 tctcaataat attgaagaga atgttcaaga gttaaagaaa actattgagg aaaatataag
     7381 tataaagaca gaagtaccag acacagcaaa agcagtgttg gagcaacagt tggtgctagc
     7441 acaagcattg gaaacctgga taaattcttt aaaagataat atgggaaatt agaattagag
     7501 aagctaactt aataatcatt aaacaaaaac cagcatattt aataatgaga tattagaaaa
     7561 aataaaacaa atgatataaa ctgaaaagta tatttaaaat aaagctcact taattgaggg
     7621 agaatgttaa actttt
//
LOCUS       DAMKMI010000167         7540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMKMI010000167 DAMKMI010000000
VERSION     DAMKMI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.173"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            86..427
                     /locus_tag="PC429_003335"
     CDS             86..427
                     /locus_tag="PC429_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF3848793.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            578..991
                     /locus_tag="PC429_003336"
     CDS             578..991
                     /locus_tag="PC429_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF3848794.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDHIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1193..1834
                     /locus_tag="PC429_003337"
     CDS             1193..1834
                     /locus_tag="PC429_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF3848795.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1858..3111
                     /locus_tag="PC429_003338"
     CDS             1858..3111
                     /locus_tag="PC429_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF3848796.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3157..3807
                     /locus_tag="PC429_003339"
     CDS             3157..3807
                     /locus_tag="PC429_003339"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF3848797.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3858..4454
                     /locus_tag="PC429_003340"
     CDS             3858..4454
                     /locus_tag="PC429_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF3848798.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4467..5306
                     /locus_tag="PC429_003341"
     CDS             4467..5306
                     /locus_tag="PC429_003341"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF3848799.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKKVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5551..6705
                     /locus_tag="PC429_003342"
     CDS             5551..6705
                     /locus_tag="PC429_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF3848800.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7011..7187
                     /locus_tag="PC429_003343"
                     /pseudo
     CDS             7011..7187
                     /locus_tag="PC429_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT         3124 a          730 c         1330 g         2356 t
ORIGIN      
        1 aaatcagtct tacatatatt tccgatagat tatattgata aaaaacacta aaattaaatt
       61 aataaaagta cttggaggga tggaaatgaa agaggaaaat tttattaatg ttgaatacga
      121 ggataaacgt ttttcaatta atagtaaaga agttttgtac atagaaaagc aagagtatga
      181 agtaaagatt tatactgaaa gtggtaatta tgaagttgga gaaaatgctc cttgtataag
      241 aaaactattg aaagaagaaa attttttcaa atgcagtaat agttatattg taaatctaga
      301 caagtgtgaa ttttatctca gcaataattg tatttttgtg aataataatt ttataagaat
      361 tttaggttca gaaagaaaaa agaaattaaa aaagaaatta gaaaaattat atgattatga
      421 agattaatat tgggaggctg tattttatac agcctcttta tttattgatt gtattattaa
      481 taaattttaa attatgataa aatataaaac aagtaactta tttcagaata aaatactttt
      541 taaaagaaag ggacgagata tgggagatta taataaaatg cttgaattaa agaataactt
      601 aggtatcagt gaaagaaagt taaaatatcc ttgtatctat aaacatttca aagggaaata
      661 ttatgctaca atgggtctta gcaaggctat tgatcatata gaaaacatat gtgaaatata
      721 tggtaaagaa aatttaatcc aaaacagaaa taaatataaa ttagttatta gacatactga
      781 aagagaagaa gatatctatg tttataggga tttagatgga aacttttatc ataaaaaaga
      841 agaagatact aatgatttag ttttatacaa gaccttatat gatgatacag gaatatttgc
      901 aagacctttg gatatgtttt tagaaaaagt agatacagat aagtatgtca attcaattca
      961 agaatacaga tttgaagaag tatataaata atataagaga aatatgttta taaatttaga
     1021 aaaattcttt aacagtacaa taatttgcag aaatacaaat gagatttatt gtaaataata
     1081 tgttagatta gattataaaa tagaaacaaa gtaaagtatt atatgatata attaagtttt
     1141 gagaagtgat aatttatatt taataaaaaa taggcatata ttaggaggac atatgggaaa
     1201 tactttttat atagtaagac atggacaaac ggattggaat atattgggaa aaacacaagg
     1261 tcatggtaat tctgacctta cgcctcaagg tatagaacag gcaaaagaac tttctgaaga
     1321 tattggcaag tattcaattg attatatttt tagtagcgac cttgggagag caatgcaaac
     1381 agctcaaatt ttaggagata agctcaatat tgaagttcaa aaaactgagg cacttagaga
     1441 aatgggattc ggagtttggg aaggcctttt aataaaagaa attcaaaaag attattcaga
     1501 tatatatgca acatggagaa atgaaccaca cttagtaaat ataccagaag gcgaaactct
     1561 taagattata aaggaaagag tagatgcttt tataaaggaa ctaaatgaaa aatatgataa
     1621 taaaaatata attcttgtta cacattcaat tacattgagg gttatgttgt tgtcattttt
     1681 agaatcaggt atggaaaata tatatagaat taagcaagat aatactgctt taaatatagt
     1741 agaatttaaa gattatgggc ctgttatagt aaaaatgaat gatacaagtc atataaaaaa
     1801 tcatgtaaag ataaataatt ctgcactgga gtaggaatca attataagga gaaatttatg
     1861 agtaaagtta tagtagttgg tggaggagct tctggaatga tggcagcttt gtcagcttca
     1921 aaaaacaata atgaagtaat attagttgag agaaatggag aattaggaag aaaacttagg
     1981 gcaactggtg gcggtagatg caattttacc aataatagag agatagaaga tttttttgat
     2041 aaagttgtca gtaataaaaa atttctctat agtagtttct atacatttac aaataaagac
     2101 cttatttcgt attttgaaag tagaaatttg gaatataaga ttgaagaaga aaatgaccac
     2161 aaagtataca ctaaaaatga taaatctata gaagtaatag aagtattaaa taaagattta
     2221 ctcaatcaca atgtaaagat aatgtataat aagaaagtta tagatattat aactgaagaa
     2281 attgctctaa aagatgattc taataaggac aagtcaaagt atctaataaa agggattatt
     2341 ttagataatg gagataaaat actaggagac aaggttatta tatcaactgg aggagtgagt
     2401 tattcaaaga cgggttcaga tggttcaatg tataagattc ttaaaaagca tggacataca
     2461 ttaaacaaac tatatcctgc tttagttcca cttacaatag aggaaaaatg gattaaagat
     2521 ttacaaggaa tatccatgaa gaatgtggaa atatcttgta agattaagaa aaggaaaata
     2581 tcaaaatctg gagacatgct atttgcccat tttgggataa caggaccttg tgttcttata
     2641 atgtcttctt atattaacaa gattattgaa aaggaaaagg tagaattaaa tatagatttc
     2701 ttaccaaatt taagtactga tgaaatctct agtataatca gagcatttcc aaataaaaat
     2761 gttctaaata atcttaagca aatactacct caaaatttct taaaagagat tttttctcta
     2821 ttatctttag ttgataaaaa ggcaagtgat ttgtctaagg cagatgaaat tagaattatt
     2881 gagtatataa aaaatatgaa gctaacttgt aatgggacta caggtataaa cactggtatg
     2941 gttacttctg gtggtatttc agttaaagaa attaattctt ctactatgga atctaaatta
     3001 gttaagaact tattttttac aggtgaagta attgatatag atgcagaaac tggtggatat
     3061 aatttacaaa tagcattttc aactggatat cttgcgggta taagtgtata aatataattt
     3121 tttaataatt aaatatgaat aaatagaggt gtatatatgg gtaatttagt tatagctgta
     3181 gatggtccag caggagctgg aaaaagtaca atagccaaga tagtagcaaa aaagctaaat
     3241 ataaattata tagatacagg tgctatgtac agagcagtta cttataagtg tttaaaaagt
     3301 ggtattgatg tcaataatga aaaagaagtt attcaaatag ctgaaaattc ggatatagat
     3361 tttaaagata acaatatata tttggacaaa gaagttataa atgaagaaat aagaacaata
     3421 gaagtaagca ataatgtttc taatgtagca aaaataaaag aagtaagaca gttgatggtt
     3481 gaagttcaac gaaaaatagg aatgaaaaat tcagtaatac tagatggtag agatataggt
     3541 tcatatgttt tcccagatgc agattacaaa ttctttttag tagctactcc agaagaaaga
     3601 ggaaatagac gctataaaga actttgtaat aaaggatata atactacttt agaagaagta
     3661 attgaggata taataagacg agatgaaatt gattcaaata gagaatttgc accattggta
     3721 aaagctaatg atgcactaga aatagatact acaggaaaaa ctatagaaga agttgtagaa
     3781 gaagttgtat ctaaaataaa tctttagttt tttatgcatc ataatgaaaa taaatgtagt
     3841 gttgaaggga gataaacgtg aatttttata gatttgtaat aaatattttt aagggttttt
     3901 ctaaaatatt ttttaaatat gaagttatag gtgcagagaa tattccagat agaggaaata
     3961 tagtaatagc atcaaatcat aaatcgaatc ttgaccctat attcttggct gctgccatag
     4021 aaaatagaga aatagctgca attgcaaaga aagaattgtt taaagtaaaa ccattgggat
     4081 ttatattaaa aaaattgcat gttatgccta taaatagaga aaaaccagat gtatctacaa
     4141 taaaaactat tttaaggtca gttagagacg gttatgtatt aggaatattt ccagagggta
     4201 ctagaataaa aggagattct tttggtaagg ctaaggcagg tctttctgta tttacaataa
     4261 aaagtaaatc taaagttgtt ccagtatcaa taatatctaa atataaactt tttagtaaag
     4321 taattgtata tattggagaa ccaatatcat ttgaagaaca ttttaaggaa aaattgagta
     4381 atgatgacca tgaaaggata tcacaagaga ttttagaagt gataaaacaa aattacttta
     4441 aatattcaaa ataagggggt aaagttatga acataaaaat agcaaaaaat gctggctttt
     4501 gttttggagt aaaaagagca atgaaaatgg catgggatga agttgaaaaa aatgattctg
     4561 gcatatatgc tttaggtcct cttattcata acaagcaagc agtagcaaaa tatgaagaaa
     4621 aaggtcttaa gactgtgaat gaaattgata caataccaaa tcatgaaaat atgataataa
     4681 ggtctcatgg agttcctgaa aatatatata aggaagccaa agataaaaaa ttaaaaattg
     4741 tggatactac atgtccattt gtaaagaaaa tacatacagt agtaagtgaa tatcataata
     4801 aaggctatga aataatagtt attggagata tgaagcatcc agaagttata ggtataaatg
     4861 gatggtgtga aaattcagct ataataataa agaccttaga acaaatggaa aatatggaat
     4921 ttgataactc caaaagatat tgtcttgttg cacagactac aataaatcca gagttgtata
     4981 taagtatagt taataaacta tcagataaat tagaagaaat agtatttaat gatacaattt
     5041 gttcagctac aaaaacaaga caagaatcag ctaaagagtt agctaaaaaa gttgattgta
     5101 tgatagttat aggaggtaaa cacagttcaa atactcaaaa gttagtaaag gtttgtgaag
     5161 acttagtacc aacttttgca atagagacta aagatgaatt ggatgtaaat acgcttaaaa
     5221 aatataaaaa tttgggtatt acagcagggg catctacacc taattggata atagaagaag
     5281 ttgtaacatt ccttgaaaat ttataatttg ataggtaata aaaataggtg gacattgtta
     5341 taatttatca taatatccac ctttgatttc atattacatg tggtaaatag ataacctaat
     5401 atcaaaaagt gtgtatctta agatgaaaaa ctaagagtat aagatgcgca ctttttaata
     5461 ttagaaattc aaaagaaata ttgatattga ttatcaatag aaaatagaat ataatataat
     5521 tgttgcaaaa ttagtctagg aggtaaatta atggaaatgt tgatatccgc ttctgaagag
     5581 gcgtttttac atgtaggttc tatgattgga ttttttattt tgttatttgg atatattaat
     5641 tataaaacta gtggaaattt tactaatatc atatcaaaaa acagaaaatt tcaaccctta
     5701 ataggtgctt taattggagc tataccaggt tgtggcggaa gtttggcaat aatgccattg
     5761 tatataaatg gaaaattaag ctttggagca ataattgcaa gtttaatagc tagtatggga
     5821 gatgcagcat ttgtacttat ttcatcaaat attaaaatgt atttttttgt cactatagtt
     5881 agtacaatta caggaataat aactggacaa ttagtggatt actttaaact agaagaaaaa
     5941 ttagggttaa aaaaccgtag aaaatcagat aaatatgata taagtaaaaa taaccctaaa
     6001 gaaaataaag aacaacataa tcatgatgaa ataattttag acactctagc taaatctcat
     6061 ggaaatacaa atagacttgc atttataata acacatggaa agggatataa aatttatata
     6121 ggaattatag taatcggttt tatatttatg tcacttgctc actctggact taatcttcct
     6181 ataattgaga agttacattc attggaagaa gtaatagcag tgattggaat attattttca
     6241 ataatttata tgtggtgttt taaaaaggta tttaaaaatt ctaatcaaaa agaggaagaa
     6301 aataaaaaga tttctttgcg agaaatgctt atccattctg ttggagaaat atcatttgta
     6361 attacatgga tttttattgc atatcttatt tacgatttga taatattaat tttaggtgga
     6421 gatgaatact tagttaaatt agtattatca acaggtgtaa taagtgtatt tataggtgca
     6481 ggtcttggac ttattccagg ttgtgggata caaattgtgc ttatgtcatt ttatttgaag
     6541 ggaactatac cacttggtgc agtgatagcc aactctattt ctcaagatgg agatgcactt
     6601 ttcccacttt tagcaatgga taaaaaatct tctttatggg ctatgattat tactactata
     6661 ccagcaatac ttgtaggtat gatagtttat gcatttttag gttaaatata attatgaata
     6721 aagaagtatt aaaaaaataa taaagcacat aaatttattt ataagaatag tgaagattaa
     6781 atataaaaat aaaaaattca atatgaatta aattattgct taaatattaa tgagaacatg
     6841 ggagataata ttattcctca tgttctctct atttaattaa aggaattatg gaacaaattt
     6901 caagtgatac tttctatcaa aactcatata taatatatat taataaatta aactataata
     6961 cgtaatacag tcagtatatt gatataaaaa acttaggagg agttgtaaaa atgggtaatt
     7021 taaaaagaca gtgtgatgtt tcaagtggaa gagaaaaagc tgacattgta ctaaaaaatg
     7081 ggacaatcat aaatgtattt acagaagagt taataacagg agatgtagca atagtaggag
     7141 acactatcgt tggaattggc gattacaagg gcaatgtaga aattgattaa aaaatagcta
     7201 cgcacactcg tgactttagt catgagttag tagctaaaat agtcagcata tagggaaacc
     7261 tatatgtaga gataggatag aacctatcca atactacttg aattgctgga aacccctaaa
     7321 gctaatcaaa ctaaaacata aggatgaaat aaacctaagt gtgaaagtta cgaaagtaga
     7381 aaaaattggt tagatggtat aaggttaaac cctaagtact ttaaaatggg caatcagcag
     7441 gtaaactccg aataggagaa acttcaacga ctattcctct tgagggaagt acactacaag
     7501 ctattggtag tggaagtggg tagaccttaa cggataatgc
//
LOCUS       DAMKMI010000168         7540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMKMI010000168 DAMKMI010000000
VERSION     DAMKMI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.174"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(72..770)
                     /gene="yunB"
                     /locus_tag="PC429_003344"
     CDS             complement(72..770)
                     /gene="yunB"
                     /locus_tag="PC429_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF3848801.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            984..3677
                     /locus_tag="PC429_003345"
     CDS             984..3677
                     /locus_tag="PC429_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF3848802.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKDDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            complement(3951..4886)
                     /locus_tag="PC429_003346"
     CDS             complement(3951..4886)
                     /locus_tag="PC429_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF3848803.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(4930..6102)
                     /locus_tag="PC429_003347"
     CDS             complement(4930..6102)
                     /locus_tag="PC429_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3848804.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGKVALIKAIKVLAMCGVDILYSK"
     gene            complement(6089..6553)
                     /locus_tag="PC429_003348"
     CDS             complement(6089..6553)
                     /locus_tag="PC429_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848805.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            complement(6568..7410)
                     /locus_tag="PC429_003349"
     CDS             complement(6568..7410)
                     /locus_tag="PC429_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF3848806.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
BASE COUNT         2559 a         1297 c         1062 g         2622 t
ORIGIN      
        1 ttaatattat ttccaaaaat tttatttata aactattatt ttgaatttat ttaaaaatat
       61 acaactttat attacttgtt ggtattcttt ccagtgttag gtaaaacatt tcctagattt
      121 ttttctggaa tatttacata actttctgga acatcaccta ctattatagt ttcacaaata
      181 ggtatttgag ctttaatttg ttttgttgaa gttataagtg gtattactac tttaacttgt
      241 gtttgagctt ctaaatatat tctatgtcta gtttgattta tacctgaaga ctcaaattcc
      301 gttttaaagt ttactgatac tgttcctata ggttcaatag atacttcaag tttaggaccg
      361 tatttagcta atataggact tcctaatgct gttcctatag gtatgtaaga acttgctgtt
      421 ttatcctttt ttaattcatc ttgtatttct aaagctattg cagatgcaat ttcattcatc
      481 attatagtgt ttgcttgtat catagttatt ttaccattat tatcaagttg tatatccatt
      541 aaatcttcat aatttatttt tccttctacc atctcagcaa cagctttatt tatcgaccta
      601 tttgctagtt caagagcttt agtctctgct aaaactgtta ttgtcggtct taaagtttta
      661 tctatatata taaaactgcc tataaataca gacaagaaaa gaattactaa aaaaatagct
      721 atgatttttc taaattcatt ttttgttttt ctatctacta ttctattcaa aattatcccc
      781 tcctatttat ttatatgtag ctttggcata atttttataa tatttgtcaa aaataaataa
      841 aataaaaata tagacttttt aatatattac gtttatacat atacaatgta cagaatatta
      901 tagcaaattc attttttcta ttaattacaa aactactcct atttgtgtta taatattgca
      961 ttaagtaagg aggttaaaat cttatgaata acgataatga taaaaatgga aataaaatca
     1021 ggagaaaaaa agttagctca tctggtaata caaataaacc aattaataga acttcagcta
     1081 acaagactcg ttcaacaaaa aacaagaaaa aatctaaaaa aagcgataaa tttaaaaaac
     1141 taagagtatt tgggatagta tttttagttt tactagttgt tggtacagca ggtactgctg
     1201 gtctagtatt tgcgtcacta agagatgtaa ctccagttac agaagctgtt ttagataaac
     1261 agacgaacca aacaactact attaaatacg ctaatggaaa aacattatct acagctccaa
     1321 gtgtaaataa aaagacaccc gttccattag ataagatttc cccttatctt caacatgctg
     1381 taattgcaat agaagatgag cgtttttatg agcataaggg tgtcgatatt aaaggtttat
     1441 ttaggtcagt attaaagaca cttacaggta ctaagcaagg tggtagtact attcctatgc
     1501 aggtatccaa aatgctgtta acaactgaac agcaaactat acctcgtaaa ataaaggata
     1561 tttactatgc acatgagatg agtaaaacag taagtaaaga taaaatacta gaaacttatt
     1621 taaataactt cttcgttggt agaggtctag caggtgctga ggctggtgca cgtggttatt
     1681 ttgataagtc tgcagctgac cttactctag cagagtcagc acttcttgct ggttcaacta
     1741 agaatccatc aagattttca gcctataaaa cttctaaatt agagggaaat gaaactaagg
     1801 atgatttaga gaataaatta ttattttttg ttaacacaac tgatgatgat ttagatgacc
     1861 ctactcaagt ggactttgat atgattgaaa aaataaaatc ttgggagctt atatctaatg
     1921 atacttatag acaattaaaa gcaggtacat tagttgttag aaaggctgta agtaatcctg
     1981 aagctaaaaa aagacaagaa attgttttaa agaaaatgtt agaattgaaa tatataaaac
     2041 aaagtgaata tgatgaagca atcaaagcta aaatagaaat aaaattgcct caatcatcag
     2101 ataaagtttc ttcatctgta gaagacctta tcgaaagtga agttataaat gctttaatgg
     2161 aacaaggaca tacaaatgat gaagctcaaa acttattcta caatggtggt ctaattgtaa
     2221 acacaactat agaccctaaa atgcaagatg ctttagaaga agaatttgat agaaatagca
     2281 atttcccagg acatatggtt ggtcctgatg gagttagcca accgcaagct gcaatggtaa
     2341 tcttagatta taaaaatggt gaaataagag cactagctgg aggaagaaat ataagtggta
     2401 gaaaaacttt aaatcgtgct actaaccctc atcagccagg ttcttctata aaacctttag
     2461 caatatatac gccagctatt gatactctaa aaataacaca agcaactgct ctaagtgatt
     2521 ctagaggagg atataagttt gaagaaaata ataaatggaa tccaagaact actactgctg
     2581 gtcatggttc tatgagttta cgtaaagcac ttgctaaatc ttcaaacacg atagctgtca
     2641 aaactgctga aatgcttgga gactcttatg atgaatgtgt agatataatg atggattact
     2701 taaagaactt tggtataaca actttaaaga ataatcactc aggttcatct gaagcaagtg
     2761 atagaaaatt cccatcattg acactaggtg gtatggcgaa tggtattact ccacttcaaa
     2821 tggctgctgc ttatggtaca ttggcaaatc acggtattta tgtagaacct tctattttta
     2881 ctactataac tacctttgat ggtcaattat tagttaaaaa tgctcctgag gaacataaag
     2941 ttgtagaccc tgaagtagct tatgttgtta cagatatgtt agaaagcgta attactgaag
     3001 gtactggtgg tgttgctaca cttccaaagg gaatgcctgt agcaggtaaa acaggtacta
     3061 caaatagtgc atatgatgct tggtttgtcg gttacactcc ttattatgtt ggtgcaactt
     3121 atattggaga tgatgctggt agaaaagatg attctggtaa cactataaaa cgtagagaag
     3181 taccacatgg aagtactagt actgcaaaac tatgggaaaa aattatggaa aaaatacatg
     3241 ctaatttaac ggttactgaa tttgaagttc ctaaaaatgt atattttaca aaaattaatt
     3301 tagaggatgg aggaaagcaa tcttctggct ctaaagcagc atttattgaa ggtactgctc
     3361 ctactaaagt aagttctcaa ccatcatctg aagatacaaa aaaaccagac aataaccaac
     3421 cagaagaaaa taataataac aataataaca caggtgataa tggtggcggt tctacacctc
     3481 ctgataatgg cggaaacaat ggtggtggct ctactactcc tcctgataat ggcggaaata
     3541 atggtggtgg ctctactact cctcctgata atggcggaaa caatggtggt ggctctacta
     3601 ctcctcctga taatggtgga aataatggtg gtggctctac tactcctcct gatggtggtg
     3661 gaactccagc aacttaataa aaaatagaat ttaaataaaa atataaggag ttgtctcaag
     3721 gtgattaaaa aaatcacttt gaaacgctcc tttttttata attacaatat attatttgta
     3781 ttcataaaaa agagaatccc aataaccaga atcctccttt ttatataaac aaataaatat
     3841 tttattttta aagtttttaa ctaagtgctt tattcttact tttattccta attgtttata
     3901 tattcttaaa tagttatatc acaaattata taaacttaat atttttctaa ttactgtata
     3961 ccaaatgttt gaaatctatt taatttgcca tatctcttaa taagcatttc ccactcttct
     4021 ttatcataga aatcacactt atttctacca aatagcttgg caacttcaat tgcaaatctc
     4081 ccagctgaat ctaaatcact aaaattagtt gcacctgtag cacatccagc aacaggcatc
     4141 tctgttgtaa ttgctactcc tactattggt gcatctgtag ctactgctgg ttgtaaaata
     4201 ctatttaagt gatacagatt atttccatat ggtgttatat cttgaataga caaagggaat
     4261 acaaatggag atttccctgt tacagtctgc ataatatcta aaaggttatc actagttttt
     4321 aatatgtagc cttctttaac agttggcgat attgcaaatc ctctagtatt gattactcta
     4381 tttcctttag tagtatctat agaaagtatt gcatcacaac tactatcaac ttcattttca
     4441 ttcatagtca tcatatcaat tggagaatcc ataaaaggag taggatagtg ttctttagtt
     4501 ggagcatctg ggcaaatatg agttgaaatt acaacatcac catctaatat atcccctttt
     4561 cgttgcatat ccaaaagttt agcagccact gcaattgcta ccaatgctcc atcaccatct
     4621 gaaacaaaac ctataacttc tggtcttgct ccaatccctc ctagtctacc taaaatgcca
     4681 agagtaggag catctccacc ttttaatttt ccatttttcc caggaattgt aagctttact
     4741 aaatctgttg agcctttact tgaagacaat tcttttacag ttacatctac ttcaccataa
     4801 ccttgtaagt aagtttttac ttcttcccca ttagcatttg ctttatctaa aatgtcataa
     4861 acctctatta cttgttttaa tatcatgtct aatctgattt cctttcttaa tatattttaa
     4921 aattatatat tatttactat acaaaatatc tactccacac attgctagca ccttaattgc
     4981 ttttataagt gctactttgc cttcttcacc accagctgca acagcaaatt catctgtatg
     5041 agttgcagta ttttctttta attttatata tgcttgtatt gcaggtatct catgagttaa
     5101 atttcctgta tcagttgaac ctataccttg acttaaatct ctttcaacat agtcttcacc
     5161 caaaacttca aaattttgtc taactatatt cactaattca gaatcattct taatctcttt
     5221 gtatatttca tctaattgaa ctatttctac attagttcca gtagaaattg cagctccttt
     5281 agcacaattt ttaagcattt cacaaatctc atttaaatat tctatgctta aagctcttac
     5341 attaaatata gctgtagatt tttcaggaat tatattatgt atgctacctc catcagttat
     5401 aattccatgt actcttgcat aatctttaag atgtaatctc atgctattta cactgttaaa
     5461 tagtgctatt actccactta aggcactttt cccttcccaa ggaaaagcag cagcatgtga
     5521 tgccttacca gtaaaatcaa attttaaatt aacttgagca aatgatatat catcaggcat
     5581 tgaagcatca cttggatgta aaattaatgc agcatctaca ttattaaata caccttcttt
     5641 aatcatagtt atttttccac ctactctttc ttctgcaggt gttccaaaaa cctttacagt
     5701 cccttctata ttatgttttt tcatgatttc ttttaatatg atacctgcac caacagaact
     5761 tgtccctatg atattgtgcc cacatgcatg tcccattcct ggaagtgcat catattcagc
     5821 taaaaaagca actgttttcc cattagttcc atttgaatac acagcttcaa aagatgtttc
     5881 taatcctcct acacctttag ttacattaaa ttcattttct tctaacaaat cacataatgc
     5941 tttttgtgac ttatactcct caaatgctaa ttctggattt gaaaataaat agtcagaaac
     6001 tttttcaaat gatgtctttt tctcttcaga taataacttt atttcctctt taatttttgc
     6061 ttttaattca ttcatattta ctacctcctt aaagcattcc atttaatctt aaaactgttt
     6121 gagatataaa agcagaacca aagtaagtag atgtcataac tataaatgat ataactacta
     6181 acttccaact caaagtcttt aatatatcta attggttacc tacagagatt cctgcaaaag
     6241 ctaataatgg tgttactgta gacataaaat tgattttccc tacatttgtt gctatgatat
     6301 cccccactgg agatattgga agcgataaaa caaatgcaac taatgtagcc catgcaaaac
     6361 ctggtaaatt aggtttttta acaaaattct ttattataat agcaattatc accaacaaaa
     6421 atccagatac acttccaatc aacaaatctt gagcctgagt cttaagtgct atcatttgtc
     6481 caaagcaaat aataatatat ataaacaata ctatttttat ctgctccata atagtcttaa
     6541 aagttttttc catatatata tcctccttta caatttatta cttgttcttc ttgtttttct
     6601 tcctctaaat ctttcaataa ctggagctac tttgctataa tagtagttgg ctactggtaa
     6661 agataagaat aattctatat atattccagt aacagctgta atcaatccac tagtagctga
     6721 atatgctaaa atctgctctg acatttgtgg aaccgtattc attaatgatg ctgttgcagc
     6781 tcccatcatt gaaccactac ccattcctga tgccattgct aaagcatatg gatgaaatcc
     6841 taaaagtaca cccactggtg ctaaaaatga aaataataat gttccaaata tactaccact
     6901 tatgtaagtt cctagtgttc ctataccttc tggtgagttt aatccatact tatctccaat
     6961 aattcctagt gctgtgtcac gacataatga aaaagtacca cctaatgcac ttcttccaag
     7021 acctaatgct actccaacag gtaatgctat caacattgta cccatatgac caaattcttg
     7081 taataaaagt gctggtcctg cttgaagtaa tataccaact ttaggccctg cattaattcc
     7141 aaacattatc cctaatggat atagtgtcaa agataataaa tatggagata catttatttc
     7201 attatcccct ataaactttt ttaaggctgg tataactctc cctaataaat gaggagaaac
     7261 agccattcct atgaatatag cccatagtat actaagtaaa gttacattaa ttcccataac
     7321 tttaaatgaa ataggtccaa ttaattctgc caatattgtg attataaagg ctgctattat
     7381 ggcatttcta atccctgctt tttctttcat ttctatacct ccttgtaatt tttataattt
     7441 tcttataata taattgttag tgattttatt atataaccta tcattgataa tgtataatat
     7501 atatttattc atcattttta tatactttta gttatgaatc
//
LOCUS       DAMKMI010000169         7467 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMKMI010000169 DAMKMI010000000
VERSION     DAMKMI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7467
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.175"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(416..805)
                     /locus_tag="PC429_003350"
     CDS             complement(416..805)
                     /locus_tag="PC429_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3848807.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(2604..4010)
                     /locus_tag="PC429_003351"
     CDS             complement(2604..4010)
                     /locus_tag="PC429_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3848808.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVEFDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(4055..4762)
                     /locus_tag="PC429_003352"
     CDS             complement(4055..4762)
                     /locus_tag="PC429_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848809.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            4933..5847
                     /locus_tag="PC429_003353"
     CDS             4933..5847
                     /locus_tag="PC429_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848810.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QKLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            5847..6608
                     /locus_tag="PC429_003354"
     CDS             5847..6608
                     /locus_tag="PC429_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848811.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMFMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            6620..7357
                     /locus_tag="PC429_003355"
     CDS             6620..7357
                     /locus_tag="PC429_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848812.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2547 a         1202 c          808 g         2910 t
ORIGIN      
        1 ttaacacttt tatctctatc tctttaatat cttatattaa tcctttattt ttattttttc
       61 aatatcatca ttaattccta tttattttat catcttaaat acgaaaacac tgtgtcatat
      121 ttgcaaaata tatattatca tctatattaa agcttcacaa tttatattta aaaattctct
      181 ttaattacac tacctctatt taaaaagcta ccttaattat acttataagt aaaggtagct
      241 ttttaaattt aattaaatag aatatccact attaattttt atttaattta tttagattca
      301 cattatagta tcataatcca cttataatca tttgtcatat tacttaagta gactaatata
      361 ttacataaat acacttacat acactttata ttgatgctat aagaaatacg tttgcttata
      421 cgtctttctc ctcttctttt agatttttta tccactcttc aataagtaag atatcatctt
      481 tgctcaaatt aatattttca tgtaatgaca acaataaact ttttatagaa gaatcatgaa
      541 tatttctaaa aaaatctctt gtctcaactc ctatgtattc cttttcttta attaaaactt
      601 cataatgcgt atatttatct atcttttgag aatttagaaa tctttttact actaatctag
      661 ataaaagagt caaagttgtc gtttgtttcc attgatattt ttgctccatt cctaaaacaa
      721 tatctctaga tgttactttg gaatctgatt tccatataaa tttcattatc tttaattctg
      781 attgtggcaa cttttttata gtcatgataa aaccttgcta ctcagaatat agacaagcaa
      841 tatccataat aaaatttttc gattaaatac atcttatacg gatttatata agctcagact
      901 tataagatat ttttcaatct taatcctaaa tttagctatt tgtacacggt aaatatatat
      961 aaataatgtc aaaattctct tgtattatat tgcttaattt tatgaaatat tttgtactca
     1021 gctatctatt ttggatactt gtccagtagc tctcctttct tttaaactct ttgattttaa
     1081 atatttgtat ttcagtaaat cctacttcct aaatttttta tttactacaa cttattactg
     1141 aacttcctac ttcctaaact ttttatttac tacaacttat tactgaactt ctcacttact
     1201 aaatttctta cttactataa attataacta aacttctcac ttactaaatt tcttacttac
     1261 tataaattac tactaaactt cctactttct aaatttctta cttgctataa attattacta
     1321 aacttcctac tttctaaatt tcttacttgc tataaattat tactaaactt cctactccct
     1381 aaatttctta cttgatacaa attattacta aacttcctac tccctaaatt tcttacttgc
     1441 tataaattat tactaaactt cctactccct aaatttctta cttgctataa attattacta
     1501 aacttcctac tttctaaatt tcttacttgc tacaaattat tactaaactt cctacttcct
     1561 aaatttctta cttgctataa attattacta ctaaacttcc cacttcctaa gtttcttact
     1621 tgctacaaat tattactaaa cttcctactt cctaaatttc ttacttacta ctacttataa
     1681 ctaaacttcc cacttcctaa atttcttact tactactact tataactaaa cttcccactt
     1741 cctaaatttc ttacttacta ccacttataa ctaaacttcc cacttcctaa atttcttgct
     1801 tactaccact tataactaaa ctttttactt cttaaatttc ttgcttacta ccacttataa
     1861 ctaaactttt tacttcttaa atttcttact tcttaagaat aatgttaatt ttaagattat
     1921 agtttttgcc ttgtttaatt gaaacattct atataattat atccttctat tttagagttt
     1981 ttttatttat atattacaaa gtttaaattt tttcattaat tatattataa atttcccata
     2041 tatttcaaca taaatacata tatatatatg ttatttctaa aaatactaca caaaaactct
     2101 actttagtag aactcaatgg cgcaaaatac ttcaatttaa aacatttatc gtactttaaa
     2161 taaataacta taataatata cttacaatag ttacatagaa gctattataa ctacacagaa
     2221 cagcgtataa ttaaaacttt tttaggatgt cattatattt tttcttaaaa ttaaaaaaca
     2281 ctataccata attctatttt agtagaattt ttttgtcaat aataaaatta aatattaact
     2341 aaaaaataaa aaaaatggaa ttatctaaag aatttaataa ccttacaata attccaaaat
     2401 attatttcta tgaaaatttt gataccatga gtaactcagc cataagcgca taaagcacaa
     2461 gttactcttt actttaataa aacttgacaa gaaaagaatt tatccttatt ctactctaat
     2521 agagttaatt tgtcaataaa gaataaaatt attttcaaaa tattttcatt cttcattctt
     2581 atctgttatt attttataaa atatcactta attagaggta attttagtat tgtttttagt
     2641 ccctgactat caatgctcgt aatatctaat ttacctctat gtgcattaat aatgccagat
     2701 actatcaaaa tacctagacc atgattttga cctttggcat atgcctcact tagtccattt
     2761 tggctcttta ataaactatt aattctttct tgactcatac cttttccatc atcttcaaca
     2821 attatatagc aaaaatctaa atcagactta gaggtaaccc ttatattaca tccttgttgg
     2881 ttatgagtta tactattttg tattaaatta ctaatagccc tttttaataa ttttttatct
     2941 gcatctataa taatatcttc atttagtata tcaaattcaa cgtaaaagtc attatccaaa
     3001 ttattattta taaactcaga aataagctct ctcaaaaacg cagaaactct aatcttatca
     3061 caattcaatg cttgcatatt atattcaagt ttagacacta aatttaggtc atttactaat
     3121 tctctgattt taatcccttg attgcatata atagacactt gctcttgttc tctatttgaa
     3181 aggctactgc tttcttcaag ttctccagca tatccaatta tcattgaaag tggagttctt
     3241 atatcatgag atattcctgc aatccagtta gacctagctt cctctttctt cctaagtttt
     3301 tcatctttca tttgcaactc tttagataca gtattaatag attttgaaat atcacttagt
     3361 attccttttt cttgtaaaag tactggttct ccttttgaca taagctttat gccagtaata
     3421 attggtttta tagatttcac catttttaaa cctatgaata gagctattaa aaaagtaact
     3481 attgcattaa ccaaaaacat acctattatt aacataggaa attgttctat ttccttaacc
     3541 gtataagtaa ggttaaactt agtgtacttg tcttttggat aacctgttac taatattcca
     3601 tttggatgtt cccatacata cacaggatag tcctttaaaa agaaccttga aaactttgct
     3661 acatctgtca aagtataatg acttggaatt tcttttggta aatcaaatcc ccatattcta
     3721 tctccattgt tatcaattaa cattgcccaa actttatttt tatgtagtac ttccttacta
     3781 gaatcatcta aaataaactt tccatttctt aaaatcaaat tatcttcaat cataatcaag
     3841 tttgatttta tttctctgct atcacttgta tctaaattat cagagtattt agatacaaat
     3901 agcatatatg aaaacagaag aaaacctgct atatttaata caataaaaag taatgaaatc
     3961 aagatacttg aacctataaa tcgcctcaat atgtacttta cactatccaa aattaaatca
     4021 ctcctcttga tttcttttat tctaatatct aaaattaatt aatcatcaac ttatatccaa
     4081 gacctcttac tgtaataaga tgtttaggtg tagatggagt tttttctatc ttctctctaa
     4141 cccttctaac atgaaccata agggtatttt catatccata catatcatct ccccaaatgg
     4201 cttgacatag tgaatcatta gtaacaattc tccctttatt ttcatacaac tttaaaagca
     4261 acgaatgctc tttagctgtt aaagaaatct cttgcccatc tttctcaaca catgcactat
     4321 ttaaattcac aacaacagaa tctcccagtt taaatactgg caactcctct tcttctatag
     4381 gcacatatac acgatttaat atacccacaa gccttaaagt aagttccttt ggtaaaaatg
     4441 gctttactat ataatcgtca gcccctaagc ccaaaccaat caatctatct tcatcttcac
     4501 ctcttgcaga gagaaaaatc actggtattt tagatgtctc tctaattttt ctcataatag
     4561 aaaaaccatc tccatctggc aatacaacat ctaaaattgc acaatctggc tttacatttc
     4621 taaataccct aacagcttcc tcataacttg atgctaaaaa tactctacta aacccctctt
     4681 ttctcaggat ttcatctatc atttttaata attccttgtg gtcatcaacc acaagtattt
     4741 ttttatcttt tattgactcc ataatcatca ccctccttta gtataccata tacccatttt
     4801 cacaactttg cctaaataga cttttaaggt tattgtaagg taaatttcag ctaattgaaa
     4861 tgtaaccaag ttattatact gatataagtt aaaagaatca ataagcacag attaaaaaag
     4921 gggatgaaac atatggaaat gataattaga acacaaagac tttcaaagga atacaataaa
     4981 atatttagag taaaggatat agacctaaga gttccacaag gagctgtata tggttttttg
     5041 ggtcctaatg gagctggaaa atctaccact cttaaaatgc ttcttggact tgctaaaccc
     5101 acacaaggaa atattaatat tttaggtaaa gagttaaatg aaaaaaatag aatatcaatt
     5161 ttaaaagata taggttcttt gatagaatct ccatcatact atggtcatct tacagggctt
     5221 gaaaacatga ccataatgca aaaactctta gatttaccta aaaaaaatgt aaatgaagca
     5281 ttaaaaattg taaggttaga aaatcaaaaa aataaaaaag ttagtcaata ttctttaggt
     5341 atgaagcaac gtcttggtat tgctatggca ataatgaagt ttcccaaatt attaatactt
     5401 gatgaaccta caaatggact cgacccagca gggattgagg aaattagaga attaataaaa
     5461 tctctaccag aaaaatacgg gatgactgtt ctaatctcta gccatctttt aagtgaaatt
     5521 gaccaaattg ccacatcaat aggaataata aatcatggcg aactggtttt tcaagatagc
     5581 ataaaagaac ttcataatag aggtcaaagt cagattgcta tcaaaactaa gaatgttgta
     5641 tctgcacaaa aactattaaa tactaaagga tatatgtcac ttatacaaga tgggtacttg
     5701 actattgaaa atttaaaaga ctcagatgtg gctaaaataa atggtatgct tgtccattca
     5761 aatatagatg tatttagaat agaagaacat aaaaaaagct tggaagatat attcttagaa
     5821 cttactggta aggcggtgac tttataatga aataccttgg tctagaattt tataaactta
     5881 aaagaaagaa gattattctg atgacctttt tatttgtagc tgttgaaata atgtggtgta
     5941 tcataaattc aaatagagct attacacgta atccagatat gttaggtgga tttgaatatt
     6001 cctatatgtt tatgagtttt gcaagtttaa atggtctatt ctttcctatc ttaatatcaa
     6061 taattacatc acgtataagc gatatagaac ataaaggaga tacatggaag cttttaaaat
     6121 cttctgtgac atctttaaat agtatttatt tatctaaatt tttatgttct gctattttgg
     6181 tatctatacc tgtattgatg caagtgttat ctattgtgtt gtttggacat tttagagggg
     6241 ttattgagtc aatttctgta tctttacttt taaaatacac attaggaaca attcttgtaa
     6301 gtattgctat cattgcacta cacctatgga ttgcaactgt aattccaaat caaatgattg
     6361 caattacatt tggcatgatt ggtagtttta taggtttgac atcaggtttg ttttatcaag
     6421 gtattcgtag attgtttata tggtcttatt acctagagtt atcaccttta agttatgctt
     6481 atgataatgt tttaggttca tctgtctata gaattgatat gaatttttct attacgattt
     6541 tagttttctt aattggaatg ctaatttatt atattggaag aaatcattta ttaaaaaaag
     6601 aagtatagga gggatttaaa tgttaaggaa gtcagttttg ggagaaattc taaagttaaa
     6661 aaattctttt atatggtatg tattattagc tttgccactt ataagtgtgt tgattggttc
     6721 tggtaatttt tatctaaatc aaggcatact caaaaaagaa tggtacagct tatggactca
     6781 agttagtctt ttttatgggg aatttttctt tcctatctta atcgcaatct tttgtgcata
     6841 tatttgtagg cttgaacata tgaatcataa ttggaataat gtaatgacac tgccagttaa
     6901 tttaaaaaat atctttttat ctaaacttac agtagtttca atacttacag ctattacaca
     6961 agttttcttt gttatctttt atatagttgc aggaaatatg tttggttttt ctggtagtgt
     7021 acctaaagaa ttgactggtt ggattgctaa aggttggatt atttctattt caatagcttc
     7081 tatacaatta tacatgtcta taaagattag aagttttgca actcctattg gtattagtct
     7141 ttgcctttct ctatttggta tgggattaat agtagcaaaa cctgctgttg gtatattttt
     7201 cccttattct atgttaggta gtgctatggg ggttataaag caatctgctt taaatgttaa
     7261 tgacactata gttacaatat gtgttgcatt tattactact atagtatttt ctgaaattgc
     7321 caatagaaat tttagaaaga atgatgttat ttcttaatgg taaatgctag atattttggg
     7381 gtttgaaatt tatattcttt ttagttgaaa tctatatcca ttttatttaa aaatatatat
     7441 cttttatttg aattttatat gttttat
//
LOCUS       DAMKMI010000170         7153 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMKMI010000170 DAMKMI010000000
VERSION     DAMKMI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.176"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            130..1023
                     /locus_tag="PC429_003356"
     CDS             130..1023
                     /locus_tag="PC429_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF3848813.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            1201..2025
                     /locus_tag="PC429_003357"
     CDS             1201..2025
                     /locus_tag="PC429_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF3848814.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEINQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            2124..2732
                     /locus_tag="PC429_003358"
     CDS             2124..2732
                     /locus_tag="PC429_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3848815.1"
                     /translation="MGKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNIEQRNGLIDFQLDLLLVDITK"
     gene            2836..3012
                     /locus_tag="PC429_003359"
     CDS             2836..3012
                     /locus_tag="PC429_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848816.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            4278..5030
                     /locus_tag="PC429_003360"
     CDS             4278..5030
                     /locus_tag="PC429_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848817.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            5020..7131
                     /locus_tag="PC429_003361"
     CDS             5020..7131
                     /locus_tag="PC429_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848818.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCAVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISIVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYKLPSIWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
BASE COUNT         2662 a          947 c          949 g         2595 t
ORIGIN      
        1 caaaatataa aaaataggat attattatag gcaataacta acatcaagcg ataacttaat
       61 acatataaaa ataaataaac aattaataaa aattaaaact tcattataaa acggaaggat
      121 agtgaaaaaa tgcaactata taacaccccc tttaatacaa acaaacactt aatctatttc
      181 tttacattta cgctagtatt gaactggata ttcagtctta cacctgcgtt gctcggaatt
      241 acaaatacaa tacttggaga aatcattttt tatcttgcat atacatcacc agctattgta
      301 ggtctatttt ttatttctac aatctatcca caaagtgcaa aacaagattt tatttataga
      361 tacctaagct ttaaagaaat tagaataaaa tggatactat taacaatatt attttttgct
      421 ttaactttta ttacttcgtt aataatcgga aattacttta atgtaaaaac ttctgatata
      481 gattggatac acatagcagt gtttagacct tctaaaatag cttatatgct attagcaagt
      541 tttatattga gtatacttag tcaagaagct ggatggagag gatatgccat tgacaaatta
      601 ttagttagat ttggatttat aggctcttca attattttag ggcttgtttg tggaatctgg
      661 tatctagggt cgtattttac acctaatcaa ataccatata atttggcaca atactcccta
      721 tttgatgctt ttttatttat accaaacatt gtacttttaa actttataat taattttgta
      781 tatatcaata caagtagaag cattttatca gctgggttag ttcatatgat gtactatttt
      841 ttcaacattc aattacttct acactaccct attaaattat gtgtaatagc tcaatatgta
      901 caaataatat ttggtttaat atttcttgta tatatgattt cttctaataa atttaaacag
      961 aaggtaaatt ctgaaattga acagataaag tcggatgatt ttgaatttga gttagactgg
     1021 taattttaat atgaattata aattaagata atctatgatt tgatacgatt actacttaaa
     1081 tataatttac tctctataat ttattgcaac atctggaaaa taattgtatt tccacaaacg
     1141 cttgaagtat ataaaaacaa aatatatact gattataagt gaaaaggagg tcatattttc
     1201 atgtttaaaa taggagaggt ttcaaaacta acacaaattt ctattcgtat gttgcgttat
     1261 tatgatgaac ttggtatttt aaaacctgca gaaatagaca aatatacagg tcatagatta
     1321 tactcagttg aacagatttc aatactacaa agaattgtat tattgagaga ttcaaaattt
     1381 agcgttgcag aaatagcaaa tatcgtacat aattggaatg atgaatttgt aattaaagaa
     1441 ttgaatagaa aaaagaatga aatacaaaag gaaataaatc aggaacaaca aagaataaat
     1501 aaaattgata aatttataga agctataaac tgtgataaag atgaaattca ttacaatgtt
     1561 gtctttaaaa agattccaag ttataaaatc atatcactta gagagataat tccagactac
     1621 caatctgaag gtatactctg ggaaaagcta tctaaattta tcaaagaaga acatatagaa
     1681 gtatcacgtc aatccaacaa taacatagct ttttaccatg atgaagaagt caaagataat
     1741 ggtgtagata ttgaagttgg aatggttgta aagaagattg gaaagaacaa atctggattt
     1801 atatatcgtg agacagaaga aattgatatg atggcatgta ctatggttta tggtccatat
     1861 gaaaatatag ctggtgcata tgaatcgttc tgttattggt tagataaaaa tagtgattat
     1921 caaatatcag gtataaatag acaaatcgga cataaaggtg aacataatga aataaatcct
     1981 gaaaattatc ttacagaaat tcaaatcccc cttataaaag tctaatagat tagtacttaa
     2041 atacttaagt actaatcacc cattgacatc ccaaaatttt catgatagta tctaaacgtg
     2101 tgttaaacat gtttaatttt tttatgggga aacttttaag gggaaaaatt atgaaagcag
     2161 attcaacaaa ggaacaatta attaatgcca caatccagtt actatcttca catagagatg
     2221 tttcaagcat tacggctaga gaaattgtag ctaaagcaaa tgtcaattta gctatgatta
     2281 attattattt taaatctaaa gaggagttaa ttaatatttc tattaaaaaa tttttaaagc
     2341 gtcatatgga tgaatataag gctacacatg atactaaaaa tttagcacct aaacagcaat
     2401 taagggaaat actaattgca ttttttgatt ttataggtga acatagtcaa tttataaaga
     2461 tttctgtacc ctatatattg atacaggaag aaattgttta tccatttgag atactacctt
     2521 taattaaggc tcactatgaa aatcagaata acaaaaaaac tgaagctgaa tacagaacta
     2581 ttgcgtatca aataacttca tttattcagt tgctttttct tcgttctaat gcttttttca
     2641 agtatagtgg tattgatatt cttaatattg aacagagaaa tggtttaata gattttcaat
     2701 tagatttatt attggtagat attacaaaat aatactatat taatttttta tgtacatttt
     2761 tttattttat ggtaacgttt gaaaatattc tcattgacta aaaatacttg cattaaataa
     2821 gggggaggaa ttttaatgtt ttttacattt ataggagtaa tatcagcaat tttattgttg
     2881 tgtacaatta tatgtggttt atggataaaa cttaataatc gtaataatga ctctaattct
     2941 cattttactt tttcaatact ttcaattgct ttttcactgt taattgtggt ggccttttta
     3001 gtaagaaatt aatgtctttg ttatgctgag gtttattatt atagattttt attattgcat
     3061 gatttttact atattatagt atttttatta tattacaggg tcttatttat tatagtgttt
     3121 tgttgttaca gcattttatt atattacaga gtcctattta ttataggatt tttattatat
     3181 tgtaaaatct tatttattat aggattttgt tatattacag acttttatta tattgaaatc
     3241 tcttatttat tgcagtgttt ctattatatt gcaaactctt atttattatg gtgttttgtt
     3301 attgcaacat ttctattata ttgtaaagtc ttatttatta cagttcttta ttatactaca
     3361 gacctttatt attacagcat ttctattaca ttgtaaagtc ttatttatta cagttcttta
     3421 ttacactaca gacttttatt attacaatct cttatttatt acagttcttt gctatactac
     3481 agacctttat tattacagca tttctattac attgcaaagt cttatttatt acagttcttt
     3541 attatactac agacttttat tattacagca tttctattac attgcaaagt cttatttatt
     3601 acagttcttt attatactac aaacctttat tattacaaca ttttattatt ttaacttctt
     3661 tactataata tagtaatatt ttaatatctc aaaatcatat caatcacttt tcagcatagt
     3721 ctaaaatact tccactttct aaaattatct atcataccta tactaataat actttttaac
     3781 ctttttatta tacatttcaa ttgtttctaa caacctattt atttccaaaa ctccaatata
     3841 ttttctcaat tacattgtgt taatagcccc taaatccttt caaattattt acaatacatt
     3901 ctattaaata atcttactgc tctataattc tttttttctt tttaacccta ctatacatta
     3961 tttacatcat ttttttcaat acaacattcg ttattctata acctttttac ttttactata
     4021 tacttctttt ctaactacac cttcaatgtt ccacatcata ttactttagc acaacaatta
     4081 atacctaaaa ataaccctct aaatcctcca ataatataat caatttaact ataataataa
     4141 cctctcacac tatccaaaca atatattttc aaacattaca aaaaaacatc caactattac
     4201 aatatagctc atatccctcc tcccctttca aatgctatca ttgacttaca agataaatga
     4261 aagtgaggat gatatatatg cttaaagtaa accaattatg taaatcatat aaaacaggca
     4321 gtaaaacata tcctgtatta aaaaatattt cttttgaaat caacaaagga gaatttgtcg
     4381 caatcatggg ttcatcagga agtggaaaaa ctactctttt aaattgtatt tcatgtttta
     4441 tcccaattga taatggggaa ataattcttg gaaataataa tataagaaat ttaaaagaaa
     4501 aggaactttc agaagttaga aatcaacaac taggatttgt attccaagac ttcatgctat
     4561 ttgatggtct tagtgtcttt gaaaatatat gtgttccaca aattatcaaa ggtagtcaag
     4621 tcttacaaat ggaaaaccgt gcaattaatc tatgtgagct atttggtatt gaacatatca
     4681 gaaaaaaata ccctgccgaa atatctggtg gagaaaaaca gcgtacagca gtagcaagag
     4741 cactcatgaa caacccagat ttaatattag cagatgaacc aactggtaac cttgatagta
     4801 agtcttgtcg tacagtcatt gaatcatttt taaaagcaaa gcaagaatta gatgctacta
     4861 tactcatggt tacacacgat agctttgcag caagcttctg tgaccgagtt attgtgctta
     4921 aagatggaat aatttataaa gaagttgtaa aatcaaattc aagacataaa ttcctagatg
     4981 aattactaga agttttaaaa gaaatgggag gcgctcaaga tgacaattaa taaattatac
     5041 agtaaacttc gagcctataa ttggaagaac tatacaactt tattcttttg tataatactt
     5101 tctgtggtat tagtaacttc atatgctcta atttatttta gcccaacaat acaaaacata
     5161 cttccaactg gtggagatag cagaaaacag gctacaatga tattctcagt tgctatatta
     5221 ggatgcgctg tctttactac atacgcttct tccctctttt taaaatttaa aagcaaggaa
     5281 attggtattt tcatggctct aggaacagat aagaagaaat taaaaaaact tctattttca
     5341 gagatattgt taatttcaat agtatgctgt tttttaggtc ttatattaag tattcctgta
     5401 tcttttggta tatggaaaat atttcaatta gtgattattg ataccagaga aatgacatat
     5461 cattttggaa ctcaaggatt catatatgga tttatattta gtgtatttgt gacattatgc
     5521 atatttatac tttctgtaaa atttattaat cgtacaaata taattgatat attaaatgac
     5581 cagagaatat ctgaaccaat acaggatgta aaggactggt atggtatatt aggtataatt
     5641 tttattatac tcggtatttt tctaggatat ctcgtacctt cactaactat aaacaaatta
     5701 aattataaac taccatctat atggagcttt acttatctac tctctcttat tggaatatat
     5761 atgattatga catacatagt tgtacatgct aaaagaggaa aacatccaga gaaatactat
     5821 aaaaacatca tatcaactag catgatgaga tttatgggaa gacaaacagt aaaaaacatg
     5881 tgtgttattt catttttgat tgcaggagca ttatttgctg tattctatgt tcctaatatg
     5941 gttacaggta tctatgatac tcttaaaaat aaccctatag attatacttt tacctacaat
     6001 caaagagata aacaggtatc aaaaaaagat attgaacaat tagcagataa acacaatata
     6061 aaaattcaaa actatatgga aattccttct atagaattaa ttgttgatgg tgtagaatca
     6121 atatatcata aagatggaac tatcgaaaag gtatatatga aaaaaagctc ctatgatatg
     6181 ttctttaatt ctaaagattt ttccaagcta attggcaaac caataactat aaaaaatggt
     6241 gaatatcttg ctgttgtaaa taaaggaaat gaaaatcaag attataaata taccaaattt
     6301 atatcaagcc cagtcacaga aaaagaaatc ccctttagat atgctggaaa aataatattt
     6361 aatggttctt tttttcaaaa tagaagtatg aacgcctatg tccttaatga tgaggattat
     6421 aaatcattta ctaaagattt atccataaaa aattacaata attctatact atttaatgta
     6481 gataaagata cctatgcttt tgccaatgac ttaaagaaag aaattataaa aagaacatct
     6541 aaggaaatgg gcatgttcag tggttatgat gaatacgtaa aaaaacgtta tgaatcaact
     6601 ggaagaaaat attttatgga tgaagaatat ccaagtggtg aaggtcattt aaaactagac
     6661 ccaaacaaca atcagttgta tcttaattgg aaatactatc ctaactttaa aattttacaa
     6721 agccaagata tgataaaaaa catggcagtc ttcttaatgt tattcatata tgtggcaatc
     6781 atatgtttta cagcagtagg aatcattgca tatacaaggg gtgttactat agctataaat
     6841 cacaagcagg tctttatgga tttaaatcga ttaggagcaa acaatgatta tataaagttc
     6901 tgtattagaa atcaattaaa gaaagtattt tcataccctc ttattgcagg aagtttttta
     6961 atttatctgt ttaattttat gattatgtta aataatgatg gtagaatcgt caatagtgag
     7021 gtcaatgctc ttttgataaa cttaggtttt atagggattt gtggggttta tatgtacctt
     7081 atatatctat taacttataa gaaattcaaa aaaattattg gtattttata ataaaaaagg
     7141 ttattagatt ttt
//
LOCUS       DAMKMI010000171         6943 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMKMI010000171 DAMKMI010000000
VERSION     DAMKMI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.177"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            70..1473
                     /locus_tag="PC429_003362"
     CDS             70..1473
                     /locus_tag="PC429_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3848819.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1692..4169
                     /locus_tag="PC429_003363"
     CDS             1692..4169
                     /locus_tag="PC429_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF3848820.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4184..4870
                     /locus_tag="PC429_003364"
     CDS             4184..4870
                     /locus_tag="PC429_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848821.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5144..5977
                     /locus_tag="PC429_003365"
     CDS             5144..5977
                     /locus_tag="PC429_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848822.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            6015..6476
                     /locus_tag="PC429_003366"
     CDS             6015..6476
                     /locus_tag="PC429_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF3848823.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6507..6755
                     /locus_tag="PC429_003367"
     CDS             6507..6755
                     /locus_tag="PC429_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3848824.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2676 a          750 c         1077 g         2440 t
ORIGIN      
        1 tatcaataat tattgtctat tttatataag caataatcaa aataaaagtt taagaaaaga
       61 gttgattaaa tgcataaacc tagttataaa acagtatttc attcatacat tatttttttc
      121 atgattttta tttgcttaat cctgacagta ataggtattt tcttatcact tattactatt
      181 cagaaaccaa atggggaatt tattcgtagt gactggccga aaaaatttac agaaaacttt
      241 aatgaacaaa tcgtatttat agataataag ccacaagtaa aacagtctgg attagaaaaa
      301 cttcgagaaa atcaattgtg gttgcaaatt ttagattcta gtggaaatag agtttatggg
      361 tttttagaac caaaagagca taaagattat tattctaatt ctgaattatt agcattagct
      421 aaccaaaata attcaagtaa tggaactgta tactttaata caattaaaaa taataaaaat
      481 gactatattt atattatata ttttccatta aatatatcta aagtgaatat gttttttgat
      541 ggaggtaaat ttataggtgg taagtcaatt gtattttcaa tcatagggat tatattttta
      601 ataattttgg tttctggaat tgcttatggt tactgggtaa caaagtgcat atcaaatatt
      661 acatctgctg ttagcgatat ttcaaaacga agctatcttc cagttaatat taaaggtgtt
      721 tttgctgata tatataatag tttaaatatt ttaaatgaag aaattcatag aagtgatgag
      781 atacaaaata aaactgatat tatgagagaa gagtggattt caaatataac tcatgattta
      841 aaaactccac tttcaccaat aaaagggtat gcagagcttc ttacagatag tagtactaat
      901 tattcacctg aaaaagtata taaatatgct tcaatcatgt taaagaatat agaatataca
      961 aatacactta taaacgattt aaaattaacc tatcaattaa aaaatggaat aatcccttta
     1021 aataaaaata attatgattt agtaagattt ttgaaagaat tatccattga tattttaaac
     1081 tatccagaat atgaatcaaa tatagttgaa tttaagagta atatagataa tattaacttt
     1141 gattttgatt gtacactatt aaaacgagct tttagtaatc ttattgtcaa tgcttttaca
     1201 tatggagata aaaatactaa agtttgtgta catataacat taaatgaaag aattgaaatc
     1261 attatatctg ataatggaaa agggatgtca agagaagaag catcaaattt gtttaatcgt
     1321 tattatagag gaagcaatac aaaacaaaaa atagaaggaa ctggtttagg actagcaata
     1381 acgaagcaaa ttattgaagt tcatggaggg aatatatctg ttgaaagtat acctggatta
     1441 ggtagctcat tttatatcac ctttcctatt taattaaggt taattaaggt caaataaaaa
     1501 atatattaag gttgatagat tattatattt atatagtagt ttatcaatct tttttattta
     1561 aggggtgatt tgattaaaaa tattataaat agaatgataa aagtatggag gagtctatta
     1621 gagatttaat ttagattaaa gtataaaaat attagattga aatatcttag ataaggaaag
     1681 aaggtgtaaa tttgaaaatt atattaaagt atattttcac aaatattaaa gaacgaaaaa
     1741 ttagaacagc agttatgtta ttgtccatag ttttatcaac agttttactt tttgtttcat
     1801 tctcaattgg attatcttat gaaagtgcac aaagaaaaat ggcaaaaggt atgtatggta
     1861 cagcgactat atctgtccaa agtaaaaatc cagatatatt aaccaattta gaagacatac
     1921 ctgatttaaa tgctataaaa tcaaaggttg gtgtattgga aagctctgct atttataata
     1981 aagggggata ctatgaagaa tttagtctta tatcagcaga cttaagtcaa ctaaataaaa
     2041 tcaacaagcc acgattagaa aatggcgata gtattactga tttttctggt gataaaatta
     2101 ttcttccaaa tcgctttaca tcaaaatata aaataaaaaa aggtgattct attactttgc
     2161 agatttatgg aaagtcttat acttttcaag ttagtgatat tgcatcttat gatacagtat
     2221 ttttgaggaa cacaagaggt gtaaatgcac tgctacccaa agagactcta tcaaaaatta
     2281 taaataaagg cagtggatat acaagagttc ttatagagtc agaagaagaa attacagaaa
     2341 atctagtaaa taaattatcc aaagaacttt ctactgaaaa atatatggta tcaaatacta
     2401 taaatgaaac taagataatt tcagatgcta gacaaaagac aatgcctttc tttttaataa
     2461 gtttttttgc attaaccctt agtatattta taatatacag cagttataag gttattacat
     2521 tagaacgtct tccttttatt ggaacattta gaagtattgg tgccaatgaa aaaactgtaa
     2581 ctcgtatatt gatgcttgaa agtatattat acggaagtat aggaggatta attgctattc
     2641 ctattggtgt agttgtttta aatttaatgc ttcatgggtt aggaaattct ctcgaacaag
     2701 gaatatcaat tccagtagta atatcaccca ttggagtgat tatatctgtt atagtagcaa
     2761 taatcgtttc ttcatttagt gcatatatac ctgttaaaaa agctagccat ttgccgataa
     2821 aaaatattgt actaggaaca gtagaagaaa agaatgtttc aaatcgttct atacttttta
     2881 taggaagtat aatgtttatt ttatctattt tacttccaag aatatcccct gaaaatacat
     2941 tgtatctagc tggtggattt tcattactgg gattaattgt agctactatt gtgttgattc
     3001 ctcttattac tgatatcatg tctatagttt ttgagtttgt ttataaaaat attttaggga
     3061 atgagggtaa attagctgct agaaatatga aaaataacaa aaatataata cagaatatca
     3121 ctttactatt tataagtatt tctgcagtta ttgcaataag tgttgttggt aactttgtca
     3181 aaacttatat aacagatgtg tttcgtgacg cagaattaca aggttttgca gatggaaaga
     3241 tgaatgaaga gtttattgaa gatgttagac atatggatgg aattaaaaaa atattacctc
     3301 tttatgttat gaataatgag atttctggta atggtgtaac attatcaaga ctagagggta
     3361 cagataatat aaaactatat aactctatgt ttggaatcaa ttatacaaat tttgagataa
     3421 aaaaacaagt aatagaagct tttaatgata aacgttctgt tattttgaat gaagatactc
     3481 taaaaaaagt aggcttatct ataggagata caataactct atctaatgat aagtatgatt
     3541 tttcttataa aatagtaggt agttttaaat cccgcgcaaa tgatgtagaa gctgttatac
     3601 catcacatta tgcagttaat gattttgata aaactaatta tggtttttta gtatatacag
     3661 cagtcaatcc agatgctgtt atgattcaga taagatattt atttggagat acatataatt
     3721 ggagtcgtac agttgaagaa tttaataatg attctttaaa tactataagt agttttttgt
     3781 ctcctatgaa taagatgacc tatttcattt ttcttcttgc tacagtaggt attattaata
     3841 atctactgat taattatatt caaaaaagac gttctatagc tatgtataaa tctataggac
     3901 taagtaataa acaaaatata aaagtgacat tgattgaagg gtttacttca ggtttacttg
     3961 gagctgttat tggtattgtg atatctatac ttgagattca aactatcttt atagttgcag
     4021 gaccaaaaat ttcaatgaaa ccagatttag attttaaaac atttattatt gttgggttat
     4081 tgggaattat agtaacttta ataggttcga tagtgcctat tataaaaggg aagaaaatga
     4141 aactgataga agaaattaag tttgaataat tatggaggtt aatatgaaaa ttacacataa
     4201 tagtttagct gtagaagcaa aaaatattat caaagaatat aaaattggaa atacaacaac
     4261 tcgagtgtta aaagaggtat ctttacaagt aatgaaaggt gaatttgttt ctatcatggg
     4321 tcaatctggt tctggcaaaa gtactctttt atatatttta ggtggtttag atactccaac
     4381 gagcggaaaa gtgtatatga atggagctga tatatcacat tttaatgatg agaaaatgag
     4441 cataatacgt cgtagaaata taggattcgt atttcaattt tataacttaa taccaaattt
     4501 aaatgtggag gaaaatataa tgcttccttt gttattagat ggtaaaaatt taaaagatta
     4561 taaaaatcaa ttagatgaaa tcttagatat agttggttta actgatagac gtaaacatac
     4621 accacgagag ttatctggtg gtcagcaaca acgtgttgct atagctcgag ctttaattgg
     4681 aaaaccagag attctatttg ctgatgagcc tactggaaat ttagatagta aaacagggat
     4741 agaaataata gatttactga ataaaataaa cagagacaat ggacaaacta taattatggt
     4801 aactcactca ccagaagcag ccaaaagtag tagtcgtact attacagttt cagatggttt
     4861 aattgtataa ggactaatta ttgtatttga gataagcatt tttaattata taaattagac
     4921 atatgtaaag tggatataag ttaaatacta taaatgtgct ataatattaa aataacagat
     4981 aaaagtacta tattttttat tgattatttt aattttattt gtttattaaa tatctaatga
     5041 gtattcatac tatctctatt attaaaagca gttaatgata tatagagata atatggattc
     5101 ttcttacttt aataaatatt cacgatagga caggatataa tatatgttaa aaaaagaaag
     5161 tattcatttt ataaataaaa ataggtcact tatagtttgt gaagacaatt ttgtttatct
     5221 cttgccttgt atacaattaa gaaattggat atcgaatttt actatatcgt ttccaaatca
     5281 aacaataatt tctgataatt acacgattat tcctcatgga agtgtaactt tggtgttttt
     5341 ttatgatgcc acagggttac atagtctatt atttggtcca actactaaac caaaaacagt
     5401 aggaaatatt gctaatagat gtgatgttat actaattata gaatttcaac ctgctggttt
     5461 tttcccgctt ataggtatac agcaaagtga acttattgat aaagttgttc cattttctat
     5521 aattaatact tcattagatt tagaaattaa aaaaatattt aatgaatctt tatctataga
     5581 taaattgatt ttaaagttgg aagaattatt gatttctaat attaaaattg aatactcata
     5641 tgagtttatt ttggcaattc agttgattat tcaaaactct ggaaatatat cttcacaaga
     5701 aatttctaaa aaagtatttt atagttctag gcatttaaac agactcttta atcagtacct
     5761 agggttgagt atgaagtcat tttctcgttt ggtacgtatt aataaatcta taaaacttct
     5821 aaacaataat aaaacttcat tgatgtctat atgtaatgaa ttaggattct atgatatacc
     5881 acattttatt aaagatttta aaattgtctg tggaataact cctcaaaaat accgagctaa
     5941 tatgtctgat ttttacagtg aaattgcaaa attttaagtt ataatactta taacaaagaa
     6001 aggatagtga taagatgaaa tttggaggag tattgctaat tgtcaaagac ataaataaat
     6061 caaaaaagtt ttatgaagat gttatggagc aaaaaatcac attagattta ggagaacatg
     6121 tatcatttga aaatggattt ggattacaaa gtaattataa agctataatt ggatatgatt
     6181 taccaatata tcaacaatca aataactttc agttgtattt tgaggttaat gacatcaaac
     6241 aatgggaaag taagataaat agaataggaa atattgaatt tttgcattat ataaaagaat
     6301 acccatgggg tcaaagaact tttaggtttt atgattttga taaaaatatt atagaaattt
     6361 cagaaagtat ggaaagcgtc ataaagaggc ttttaaaaca aggtttagct ttagaagaaa
     6421 tttcaaaacg cactatgtat ccagttgaat ttattacaca atttcaacaa ttataaaata
     6481 ttaaaatata taattttttc tttatattgc aaaaaacaaa ggcttggggc ataatagaac
     6541 cagaaaaacc tgaatctcaa cagcaaatgg ctcatatgga tttcgctgtt aatgatttag
     6601 aagaagcagt tcaatatgca attcattgtg gagctacaat cgcagaggag caatttactg
     6661 atgactggag agttatgatt gacccagctg gacacccttt ttgcttatgt cagatgaaat
     6721 caattatgga aagtagccat ttttcattgt tatagaccaa aatattgttt attaattaca
     6781 aaaaagcaca acagtatata ccgttgtgct tttacttatt atacatgaat acactaaaaa
     6841 atataaatag tagagcttta tcaataatga taatttttaa aaaatagtat ttttttattg
     6901 aataatttct tagatatttt aaagtatata aattttgcat ata
//
LOCUS       DAMKMI010000172         6818 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.178, whole genome shotgun sequence.
ACCESSION   DAMKMI010000172 DAMKMI010000000
VERSION     DAMKMI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.178"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..138)
                     /locus_tag="PC429_003368"
     CDS             complement(<1..138)
                     /locus_tag="PC429_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3848825.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KN"
     gene            complement(786..1193)
                     /locus_tag="PC429_003369"
     CDS             complement(786..1193)
                     /locus_tag="PC429_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848826.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1667..2551)
                     /locus_tag="PC429_003370"
     CDS             complement(1667..2551)
                     /locus_tag="PC429_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3848827.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2842..3318)
                     /locus_tag="PC429_003371"
     CDS             complement(2842..3318)
                     /locus_tag="PC429_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF3848828.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3584..4759
                     /locus_tag="PC429_003372"
     CDS             3584..4759
                     /locus_tag="PC429_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF3848829.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4840..5643)
                     /gene="proC"
                     /locus_tag="PC429_003373"
     CDS             complement(4840..5643)
                     /gene="proC"
                     /locus_tag="PC429_003373"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF3848830.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5683..6267)
                     /locus_tag="PC429_003374"
     CDS             complement(5683..6267)
                     /locus_tag="PC429_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848831.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2247 a         1148 c          757 g         2666 t
ORIGIN      
        1 gtttttataa acatctattc ttttagcaag atacttatta tatacaaatc tacaacatcc
       61 aaaagttttg ttaattaact cttgttgttt tttatttgga tacattctaa acttataagc
      121 ttttttcact gctatcacct cactttaatt gttttttgat acacttttta atgattttat
      181 cacattttat cacatttaat atattatata atgctgtggt aaacaattta ttaatcacct
      241 ataataatgt taacaaatgt aattatttaa aacaattatt tagaaggttt tatatttaat
      301 tttaaataac atatttaaga accgtaagtc ttaactttta tcattttgag gttgtcgttc
      361 acataagttc gctactacct atgcagttct cttatgaact tcttacattt tcatgtaagc
      421 acagactata tcttatcctt cagcattatc cgttaaggtc tacccacttc cactaccaat
      481 agcttgtagt gtacttccct caagaggaat agtcgttgaa gtttctcctg ttcggagttt
      541 acctgctgat tgcccatttt aaagtactta ggatttaacc ttataccatc taaccaattt
      601 tttctacttt cgtaactttc acacttaggt ttatttcatc cttatgtttt agtttgatta
      661 gctttagggg tttccagcaa ttcaagtagt attggatagg ttctatccta tctctacata
      721 taggtttccc tatatgctga ctattttagc tactaactca tgactaaagt cacgagtgtg
      781 tgtagctatt ttttaatcaa tattttagta aattttacgg taagtaaaaa cctctctcct
      841 tcaaatccta tagcaaatat acttacaagt tgctttgaga aaataactcc taatatagct
      901 acaactatgc atataccaac tattatattt aaaacattgt ttaaaaactt tagtgcttgc
      961 ttttcacctt gtttactact tatatcttga tacattggaa taaaagtagt aactatagca
     1021 gtacctattg ctgcaaatat aatatttggt atattcatag ctgttaaata actctctgta
     1081 tataagcctg taccataaat tgatgataat actaattctc tacccattcc aagaatctta
     1141 gagattatag taactatcat caaataaaaa gttgctttag ctaccttgct cattttatca
     1201 ctccacttct aacatacctc tttttaatca agtaaaattt agctcattag atataattct
     1261 atcaactaat ttgccttgct tagccctatc aaaatataat taacacctat ttcttaatat
     1321 atcacaaact acattattct tcatattttt ttatttctcc aataagtttt acatttttcc
     1381 tatataaatt gtatagcttc aattatattc aaagaaatgg agttgctacc cgataacttt
     1441 aaaagtaaaa aacagatttt attatctttt taaactatat tctaacatat tttgtccata
     1501 ttgagaaatt ttactaaact attatgtact ttattaaata aataaaataa cagcacacct
     1561 ataaaataca agtgtgctgt tcataaacag attttataaa aaaataattc ctaagtagag
     1621 attaacatgc tttctcatac tctcatatat aatgtgtcta aaaatattaa taaaaatgtt
     1681 ttaattgttt taacttactt ggatgtctta attttcttat tgctttagct tctatctgtc
     1741 ttattcgttc tctggtaact ccaaatacct ttccaatctg ttctagtgtt ttagggtcat
     1801 catcatcaat tccaaatctc attcttaaaa cttgctgttc ttgctctcca agacctgtaa
     1861 gtagttcttc tatcttttct tttaaattat gctgttcaac ttcgtgtatt acaacatctt
     1921 taaaatcagc atctgatggt ataaattcaa ctaaactact atcctcatct tcttttaatg
     1981 gagtatccaa agatacaaca tttttatctc ttcttaataa ttccaacact ttgtcaactt
     2041 ctaagccaca tgcatctgca atctcatcta tagttggctc tcttaataat acatttaaaa
     2101 gttcttgttt ctttttcttt acaaaattta ttctctgatg aagatgtata ggtatcctaa
     2161 tagtattttc gcaatcgtcg atatatcttg ttatactttg ttttatccac caagttgcat
     2221 aagtgctaaa tttgtatcct tttttataat cgtacttttc tacagctttt ataagtccta
     2281 tatttcctgc ttgaattaaa tccaacatat caatactatc tcttttatat ctctttgcta
     2341 tgctcactac caacctataa tttgaattta taaatttttc ttttgctact gaagatgagt
     2401 tgattatttc cttagccagc tctacctctt ctcctgctga caacattttg tattcctcta
     2461 tttcctttaa atacattttc atcgcattcg atacatttga tgaacccaca caaatataat
     2521 tttcataatt attttctttt ttagtatcca taatcaaacc ccttctattc atagaatttg
     2581 ctaaaacaaa aataaataat ttaaacctac ataatctaca attacataat atcccattct
     2641 ttaaatgaat tgttttgtat attatgtata caaatttata aaaactatac ctataatatt
     2701 attctacttc taacagcaat atcctcctta tatatataat ttattttatt atatgttgtt
     2761 atattcttag ttttttgaat gatatatgtc taaaaaaaga ctgtttacta aacagtcttt
     2821 ttttatatat atgctcaata tttaattttc atgatgatga ttacatccac atccacaatt
     2881 ttcatcatgg tgatgatgtt cttctaaatc ttttacctgt tgttccattt catgaaactc
     2941 ttttaccatt atatcataag ttggaagagc tgcctcagta tcgtattctt cccctctcca
     3001 atcagcgtac atcatagcat ctccattagt taaaaccaat ctacctgaca tagttatatt
     3061 atccacttct tctaaaaatt ctatttgttt ttgtaaaact tctccttcta actccacatc
     3121 ttcatctaaa cattgcacta tcattacagg tgcataataa tcttcttcat ctttaacatt
     3181 tacaacaata tctaaaattc cttctgcacc ttcttcaaat tctgccaact caacatttgt
     3241 atcaatcata tcttgaggta ctaataaatc ttctattaaa tattttatta ctctatcttt
     3301 tactaattct tttgacattt atttttctcc ttccgattct ataacattat cataatcatt
     3361 tctatattat agtatactac attaattgtt ttttcaatct cttacaaaaa ttatttttta
     3421 ttatcttcct tatattccct tttttattac aaagttgtaa tcgcattgat ttaaaaaata
     3481 gtttatattt agtataatag aaatgtagca catattttta ttcaatatat taataaaaaa
     3541 atatattgaa aatgtaatgt atgttaaatc taggaggaat attatgaagt ttaaaaaaca
     3601 aattagttct attgttgtgt ctactatgct tgtacttggg agtatgaacc tttcttatgc
     3661 agacggtaca aatgtagtta cacttggagc taacttatca gagtctcaaa agcagcaaat
     3721 gcttaattac tttggagtaa aaaaagacca agtcttagtt ttagatgtaa caaatgcaga
     3781 agaaagacag tatttacaag gtgttgcaac agagggtcaa cttggaaaag ttactatctc
     3841 ttgctcatac gttgaaccta ctaaaaaagg aaatggaatt aatgttaaaa cagcaaactt
     3901 aacttgggta actagttcaa tgattgcttc aacccttaca acagctggtc ttgaagatgc
     3961 aaatgttatt gctgctgctc catatcctgt aagtggtact ggagcattga caggaatcat
     4021 gaaagcattt gaggatgctt caggaaagaa acttgatgaa actaagaaag aaattgcttc
     4081 tgaagaattg gtagttacag gtgacttagg agatgatata gggcaagaaa aagcaacagg
     4141 tgtaatgaat gatataaaaa cagaaattgt aaaaaatggt acaaaagata ccaatcaaat
     4201 agcaaataca ataaataatg ttgtaaataa ttacaatatt actattacac cagaacaaaa
     4261 agaacaaatt aaaggtgtaa tgaaaaaaat agctgaccaa aattacgatt acaatagcat
     4321 gaaaaataca ctagataatg tatcagataa tgtaaatgaa caattaaaga aattaggtga
     4381 aagtgttaag ggttctggta tactagatac tattggtgga tggtttagtg gaataggaga
     4441 ctggttctct ggtttattct caggcggaga taaaaaagat ttgggaatct taaataatac
     4501 taatgatgag tctcttggct ctgatgctgt aataaattct acagaaggtg ttcaaataaa
     4561 tccacaaaat aatactaatt ctaacaatga tagtgataca aatagctcaa aaaatgatac
     4621 ttcaagtaat aatgaaaata acgatagttc tcaaacaaat gaaaacaaca caacaaataa
     4681 ttcagaaaat caatcaaata ataatgactc agttggtttc tttgaaaaaa taaaaaattg
     4741 gctttcaggc ttattttaga aattaaacaa ataaaaagaa ttgcctaaaa tggaaatttt
     4801 ctataaaagg caattctctt tatttacaat aaatttttat tactcactca tttttttaga
     4861 tttttctata catgcacata ttgcttttat cacagaagct ctaaaacctt cttcttcaag
     4921 tactttaact gcttctatag tagttccacc aggtgaacat accatatctt ttaattcacc
     4981 aggatgtttt cctgtctcaa gtaccatttt tgcagaaccc ataacagcct gtgatgcaaa
     5041 cttatatgct tgtggtcttg gcattcctgc aattactgct gcatctgcta tagcttctat
     5101 aaataaaaat acatatgctg gtgcagaacc acttgcacca attacagctt ctataagata
     5161 ctctggtact acctcagtat ttccaaaaga attaaatact tctgtaactg cttctaagtc
     5221 ttctttgttt atatttttat ttatagaaag agaagacatg gcttcattta caagagctgg
     5281 agtatttggc atagttctaa ctattttttt atcttctcct ataatagact ctatatcttt
     5341 tatagatttt cctgctgcta ttgtaactat aattttattg tcatctataa agtcttttat
     5401 ctcttccaaa atatcatcat atatatctgg tttaacagct acaattacta tatcagaggt
     5461 tctagttact tccttcgagt cttttgtagt atttattcca aaatttgcat ctattctatc
     5521 aagagttcct tgactatatg ctgatgctat aaccatatta ggttcaacta attttgaatt
     5581 tactattccc cctatcatag cgcttgccat atttcctgca cctatgaatc caattttttt
     5641 cactttagta cctccttatt gactaatagt aatcgttaaa atttaagctt ctttaactgc
     5701 atcagacttt tcaaatggat ttggtatact tactaccaaa attttcacta ccttattagt
     5761 atagttgacc caattatgat tgttcttttc actattatag tgtattgtgt ctcctggata
     5821 catttctatt tgttcatctc ccaaaaacac tgtaagagta ccctctaata catacacaaa
     5881 ttcctcacct tcatgtacgt aacaacaaat ttcctcacta ctcttacttg gaagaatttc
     5941 aattaatctt ggtagcattg ttttttcctt taaactagat gataaatgat aatgtataaa
     6001 tgttgagttt tctacatcaa atacttcttt ttcataactt cttaatacag ctcttttatg
     6061 ctcttttggc ttcataaaaa agtaagttaa ttctacatct agtacacttg ctatttttcc
     6121 taaggaatct gttgccacac ttgttaatcc tctttcaagt tgtgataaaa aaccaattga
     6181 caagtttgtt ttttcactca tatccttcag cgtcatttgc ttttgagttc tgagttgctt
     6241 tatttttgct cctatgtcct tattcaaaac catcacccct aaataatttt aattttttca
     6301 taatataaat acaaaaatat aaatataggt actgaaccta tctttatttc atttaaaaaa
     6361 atttttataa gtattactat aatttatcaa tctttttttg tctacatttt tacgattatt
     6421 tacgatttat ttttcacata tgtatattta atcttgccaa ctaaagatac attttttata
     6481 agcatttact ctttatataa taaaacttaa gttgttcatg agtcttcttc tggtttaatt
     6541 attaaaattt caaattatat tattatttct acacattatt acatccttta gtacaattat
     6601 aacataattt tacaatttaa tgatatctat tcaatgtctt tttttactct acaacatatt
     6661 ttattttaga gtttaatact atatatattt tttatataca gccttgtcat tttttagatt
     6721 gtcaagagtt attgaagtat tttatgtaga tttagatttt attttctaat ttctcattta
     6781 acaagaattg cttatgataa aaaataatat catctcaa
//
LOCUS       DAMKMI010000173         6744 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMKMI010000173 DAMKMI010000000
VERSION     DAMKMI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.179"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            204..1037
                     /locus_tag="PC429_003375"
     CDS             204..1037
                     /locus_tag="PC429_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF3848832.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1130..2062
                     /locus_tag="PC429_003376"
     CDS             1130..2062
                     /locus_tag="PC429_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3848833.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEVLKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2114..2596
                     /locus_tag="PC429_003377"
     CDS             2114..2596
                     /locus_tag="PC429_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3848834.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQESMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2608..3477
                     /locus_tag="PC429_003378"
     CDS             2608..3477
                     /locus_tag="PC429_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF3848835.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIRAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3520..4275
                     /locus_tag="PC429_003379"
     CDS             3520..4275
                     /locus_tag="PC429_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF3848836.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSITKIKGLKDKTLENKLNNELLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSIYIKTISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4317..5138
                     /locus_tag="PC429_003380"
     CDS             4317..5138
                     /locus_tag="PC429_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF3848837.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5143..5904
                     /locus_tag="PC429_003381"
     CDS             5143..5904
                     /locus_tag="PC429_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF3848838.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKYKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSDCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6065..6322
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6435..6674
                     /locus_tag="PC429_003382"
     CDS             6435..6674
                     /locus_tag="PC429_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF3848839.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         2981 a          631 c         1136 g         1996 t
ORIGIN      
        1 acactatata atactcaatt ttcaacattc tatacaatta tacagaaatt atacagcata
       61 ataatcaccg aaataacaaa ttttaaaaat taagcaaaac tttttgtcga agttcaacgt
      121 ctatatatat gtagtgtaaa tttttgggat aaaatactac aaaaatataa atgtaaaaat
      181 atagtaatat tggaggtcga acgatgagaa ataaaaagaa aaaaataaat agaaagaagt
      241 tgtatttgct tttaagtgct attgtagtgt gtctggcttt tatagtagtt ggtgttcgcg
      301 tcagctcttt aaatatgaag gaaaatgaaa ttagtagaaa tgcaaaactg tcaagttcta
      361 ctattacaga tatagtatct aatacatcaa tagaagttag taaagggcca agtaaaagtt
      421 ctggaaaaat agcatacata actatagatg atggtccatc taaatttact gacgagatga
      481 taaaaacttt aaataaatac aatgttaaag caactttttt tatgatagat gggaatatga
      541 aggaatatcc acagcaagtt aaaaatataa ttaaaaatgg gaacacagca ggttttcata
      601 gtgtatcaca tgatattcac aagctttatg ttactagtac atcagcaaaa gaggaatttg
      661 atacaaatga tcaaactttt tataagataa ctggtaaaca ttcaaaagta ataagaatac
      721 cttatggaag caaaccatat acaccacaag cctcttatca ggctttagtt gatgctggat
      781 ataaaatttg ggactgggac ttagatacag aagactggag atctaattct tctcaaattg
      841 tacaaaatgt aaaaaatcat attaagaata gaaaaggtga agataaagac caacttgtag
      901 ttttaatgca tgagaaaaaa cagagtgcag aagctttaga ttcagtttta aaattcttat
      961 cagatgaagg ttatgaattt gcgccagtag atcaaaacca aataccaaaa aattattggt
     1021 tacgtaattt agagtagtta aaatataaga atattgattg atatttttag aaattgagaa
     1081 taaaaatgag tgaggtgtag gtatattgaa taaaaaaatt tatataggaa tggtcggaat
     1141 attgtctttg atgatggttg gttgtaataa aagtttagca aaagtaaatg atgtggaaat
     1201 cacaaaagaa cagtacaaaa aaacaaaagc agttttatct gctactaata attatataaa
     1261 tgggcagtct ttagatgaat tagaaaaaac tttggataaa aaaggtagaa ataaattaga
     1321 aaatgttata atatcgttta tggtggataa tgaactttta tatcaagagg ctaaggataa
     1381 aggactaact ccaagtaaaa gtgaagtaga ctcaaaatat caagaactag aagataagat
     1441 gaatttgaat acaagctata aggaaaaaat ggacaaagca ggtgttgata aagattattt
     1501 aaaacaagaa atatctagag atttagcaat agataaaaat aaaaaggcct ttgaagatag
     1561 aataaacata agtgataatg atatggaagc ctactataca agtcataaaa aagattttaa
     1621 tgtagaagaa gtaagtgctt ctcagatact tatatcgact ttagataaaa ataaaaaaga
     1681 agtaagcaaa gataaaaaag aagttttgaa gaagaaggca gataatatat tgactaaaat
     1741 caaaaatgga gaaagttttg aaagtttggc taaaaaatat tcggatgata aagcaactgg
     1801 aaaaaatgga ggacagctag gatattttac aaaggatgat aaaaatgctg agtttacaaa
     1861 ggaagtgttc aaattaaaga aaaatgaagt ttcaaatgtg tttgaaacta gttatggata
     1921 tcatatagtc aaagttactg ataaaagaga aagacaaaaa agttttaatg agtgtcagag
     1981 tttaataaga gaatctattt taaatgaaaa gtatattgag catataaaaa aactgaatga
     2041 agatgctaaa atagacagat agaatttcta acagctcaat cctagcctgt aatcggaggg
     2101 agattttaca ggaatgttta agagtttaaa gagaacgaaa gttgagaaat acattatcga
     2161 taataaagac tctttttata gaatagcata tagttatacg aagaatgaag aagatgcctt
     2221 agatgtggtt caagaatcca tgtataaggc actatactca gtggaaaata ttaaagaagt
     2281 aaattatata aagacatggt tttataaaat tttagttaga acgagtatag attttattag
     2341 aaaaaaccgt aaatataata atatgacaga tattgacctt atagatgaga ctggagagta
     2401 tgataaatat actgatttgg atttaagaag agctttggaa gaattaccta ttgaatacaa
     2461 atctattata atattaagat tttttgaaga tttaaagatt gaagaagtag caataatatt
     2521 ggatgaaaat gtgaatactg ttaaaactag gttatatact gctttaaaaa aactaaaatt
     2581 aaaaattgaa gagtagggat aaaaagaatg acaagcaaag aaaggttaaa taagttaaaa
     2641 gatgagtatc ataaaattcc aatacccaag aaattagata ctataataaa tcatgaaaaa
     2701 atagaaaaca tatatcgaga gaaaaataaa aaagtaaata gattaagatt taaagttgca
     2761 atagcctttg catgtatatt tacagtatta gtaaatataa gtcctgtatt tgcagacaat
     2821 ttttctaaga tacctgtaat tagagcaata gtagaagtaa taactataaa aaattatagt
     2881 ttaaaaagtg aaaattatga agctgagatt gatattccta aaatacgagg tttaaaagat
     2941 aaaaacttag aacaaagatt aaacagtagt tttatggaag atggaaaaag actttatcat
     3001 caattccaag agagaatgga gaagatacaa tcatcaaaga ataaaggata taaatcttta
     3061 agtttgagtt atagtgtaaa aaataattct aaaaaatttc tatcaataga gatgacaaaa
     3121 aatgagatag aggcttcatc atatgtgagt aaggtacatt atactattga taaaaaaaga
     3181 caaatagttt tgactttgcc aatgcttttt aaagatgata agtatataaa agttataagt
     3241 gataatataa aagaacaaat gagagaacaa atgaaaaagg atagtacaaa atcctatttt
     3301 atagatcaaa aaaaagactt gcctgttgaa gattttaaaa caattaataa gtatcaggat
     3361 ttttatttta ataaaaatga agaccttgta atttgttttg atgaatatga agtagcacct
     3421 ggttatatgg gcgcagttga gtttgtaata ccatataaag taataaaaga tttgtaaatt
     3481 gaatagtagt ttatatataa atcttttaag gaggacaata tgactacata taaaaaactt
     3541 aaaaaatcta tatttaaaat tgctatattt tcaatatgct tattagtttc attagtagat
     3601 acaaacccta tatttgcagg tagctccttt aatgaaactt caaatagtga tacgataaag
     3661 gtgttaaatg ctaagagtat caactttaaa caagacaatt atgaagtgaa tgtgagtatt
     3721 actaaaataa aaggtttaaa agataaaact ttagagaata aattaaacaa tgagcttttg
     3781 caagatggga aaaaaattta cagtgaattt caaaataaaa tgaaaaagga aaataataag
     3841 ggtcatagat acctaaatgt aatccataat gtcaaaaata attctaagga ctttttatca
     3901 atagaaatga caaaagaaga gatagaagct tcctcatcta taactaagaa gcattacact
     3961 gttgataaaa atagaggaat actattaaat ttaccaatat tttttaaaga tagtatatat
     4021 attaaaacta taagtgataa tataaaagag cagatgagaa atcaaattaa gaaagatgat
     4081 acaaaatctt attttataga ccaagataaa gactccccag tgaaagattt tgaatctata
     4141 aataagtatc aagattttta ttttaataaa aatggaaatc ttgtgattag ttttgatgaa
     4201 tatgaagtag cacctggtta tatgggagca gtagaatttg tgataccaaa taaagtaata
     4261 aaaagactaa gatagatata tagaagattt ttagatagac tggtaaggag aattatatga
     4321 atatatatga acaagcaata agattaatag aaaataatga ggatttcgca tttgctacaa
     4381 taacgtctca ttcaggttca acaccacgtg aaactggagc aatgatgatt gtgaaaaatg
     4441 attctactat atttggctca gtaggaggag gtagtgtaga agcagaatgt ataaaacatg
     4501 caattaatgt tataaaaaac agagaatcaa tgttgtataa atttactcta aacaaatctg
     4561 atgtggcaaa actaggaatg atatgtggag gtactggaga aatacaaata gattttatag
     4621 atagtaaatt aaaaagtaat atagaaaaat ttaataagag actaaaggaa aatacaagta
     4681 aagcatatat atttggtgca ggtcatattt caagagatgt tgctgtgata ctatctcttt
     4741 tagaatttag aacagtagta atagatgata gagaagaatt tgctaatcat gaaaggtttc
     4801 cagaatcaga agtagtagtt ttagattctt ttgaaaacat accagatttt ccaactgacg
     4861 aaaatagtta tataattatt ttaacaagag gtcatctata tgattcaagt gctttagaat
     4921 gggctcttaa aagagaggct ggttatatag gtatgattgg aagtagaaca aaaattggat
     4981 tgacttacga aaaactcatg aaaaaaggtt ttaagaagga agaattatca aaagtacatg
     5041 caccaatagg cataaaattg aatgctcaaa caccagctga aatagctgta tgtatagctg
     5101 ctgaacttat aaattgtaga gctaataagg aaaaataaac taatgaacac agggagtaaa
     5161 gatattgata ctttaaaggt taattttaga gggattgaaa taaaagcagt tttagtatat
     5221 aaaaatagaa aaaatatttc tataaaaata gatccatttg gcaaaataac tgtaatgagt
     5281 cctcctaaaa tatccaaaaa aataataaaa gatataatta ttgaaaaagg agactggata
     5341 cttaaaaagt cagaaaaata taaaggaaga gaggaagtat ataaacaaag aatgtttata
     5401 actggcgaaa aatttcttta tttaggtgaa gagtaccatc tagttataaa agaactgttg
     5461 agagattctg tcaaaaaaag tgactgtaga ataactatta acgaatatca aatagttgtt
     5521 cagacaaatg atacaagtac agattttatt aaaaaatctt taaaatcttg gtataagatg
     5581 gaaagtgaaa gaatagtatt agagagaata gattttctta aattaaattg tgaaataatg
     5641 aagcaattag ttccagcgag tgttaaggta aaagaacaaa ataagagatg gggaagttgt
     5701 acagctcaaa aaaatattta tataaactca aaaatatcta tggcaagatt agatgtaatt
     5761 gattatgtaa taatacatga gtttagccat ttagtacata tgaatcactc taaaaaattc
     5821 tatgatttag taaaatccat tatgcccgat tataaagata aagaaaattg gctaaaaata
     5881 aatggatata aaataataat ctgaaaacga ttattccaaa atataggcgt aaagtataaa
     5941 agaagtatta ttatgaaaaa aataaataat ctgaaaattt taaaaaaagt cattgacatt
     6001 gtaaatgata gggaatataa tctatattaa caacaaaaca gaattaaaat ataaaattaa
     6061 aaaattaaaa gcaatgacag agacaacaat actaatagtg ttatccacag agaaccaatg
     6121 ttgctgagaa ttggtgttaa cagtagtata atgatcactc tggagctttt gtactgaata
     6181 tttttagtaa gtacaaacgt atgtcaacgt tacatgacta aggttaaggt aattaattac
     6241 ccgacctgaa aaaagagggg tagatgtatt ttttatatct acaaataagg tggtaccgcg
     6301 gaatataact ttcgtcctta taagtaatat ttatattgct tataaagatg aaagtttttt
     6361 tattttaagc gatataaata aatttaatcc atataaaatt aataaaaaaa taataaaaat
     6421 aaggagggat ttttatggaa aatactgtat atgctaaatt aaaccaagga atagattctt
     6481 ttttaagagt ggctatgact ctaagaagaa gagaggttga tatacaatca ataagtatga
     6541 ctgtagataa tatgaataat tcaggtataa aattaatagt aaatgaagaa aagacatctt
     6601 tagattctgt cttaaaccat atgaaaaaac tacatgatat aagagagata actgttgaaa
     6661 gaatgagaca ttaattaaat atattttata aaactaaaga taaatatgct tattttaaaa
     6721 taaaaaacta aagataaata ttaa
//
LOCUS       DAMKMI010000174         6744 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMKMI010000174 DAMKMI010000000
VERSION     DAMKMI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.180"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..107
                     /locus_tag="PC429_003383"
     CDS             <1..107
                     /locus_tag="PC429_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3848840.1"
                     /translation="EWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            234..1136
                     /locus_tag="PC429_003384"
     CDS             234..1136
                     /locus_tag="PC429_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF3848841.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1748..2761
                     /locus_tag="PC429_003385"
     CDS             1748..2761
                     /locus_tag="PC429_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF3848842.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPSDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            3117..3686
                     /locus_tag="PC429_003386"
     CDS             3117..3686
                     /locus_tag="PC429_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF3848843.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYFNRYN"
     gene            complement(4022..5359)
                     /locus_tag="PC429_003387"
     CDS             complement(4022..5359)
                     /locus_tag="PC429_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3848844.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMIFYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(5803..6513)
                     /locus_tag="PC429_003388"
     CDS             complement(5803..6513)
                     /locus_tag="PC429_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848845.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLEP"
BASE COUNT         2500 a          713 c         1045 g         2486 t
ORIGIN      
        1 gagaatggat ttgtcctagt tgtaatgaaa ctcacgatag agatataaat gcaagtataa
       61 atatattaaa agaaggatta agactaataa caattcaaaa taaataactt atgtaagaac
      121 cgtaggaact acggggatag cctgtggaga attagtaaga catatgttag tatgcaaaat
      181 tctatgaagc aggaaccctg tgactttagt catgggaggt tcagattgga aatgtgttgc
      241 cacaagatta ttatggatta atagtgattt taggtgttat tcttggagtt atgggggcat
      301 tttacaactg gtttactcta tatgtgcagt ctttatacaa gaagattcct aaaattggta
      361 cgtttggaaa actgttgatt ccatttataa tggctggtat tcttgggctt gttatgcctg
      421 atgttctggg gagtggacac aatttagttg aagaacttac aagtcatgaa tatatttttg
      481 gaatggttgt tttgattttt gttatacgtt ttatattttc ggctgttagt tttgggtcag
      541 gtgcaccagg gggaatattt tttccattgc ttgttttagg agcatttata ggtggaatat
      601 ttggtatggt aggtgttaaa gtttttggaa tgaatcccga ttatgtaaat aactttgtgc
      661 ttttggctat ggcaggatat tttacagcta ttgtgcgtgc ccctttgact ggtatcatat
      721 taatttttga aatgacaggt tctgttagtc aaatgctttc actatcagta atttcaattg
      781 tggcatatat tgtggctaca cttatgaagt ctaaacctat ttatgaaagt cttcttgaga
      841 ggttacttga aaaaaatgga gaacctgtac ataaagagag gggcgaaaag atattagacc
      901 agttctctgt tatgtttggc tctcctgttg cgaacaaggc aattcaggat gtacagtggc
      961 ctgaaaattg tcttttggta gcaattcaaa gaggtggaga agaaattatt cctagaggta
     1021 agacaattct tagaccaagt gatgtaattg tgactatgac agatgagaga gatagtggtg
     1081 ctgtgtatga taagatggaa aatttgtgta aagaacatac gtcacatatt ttttagggat
     1141 aatgatgaat gtttaataat gatagaatat ttaatgaaga tttgattaaa aaattggtaa
     1201 tttgaaagag agttaaggaa ttaatataag aaaatgaaaa tgtgatatct tagatgtaaa
     1261 agagtttgaa gaaaataatt gtatatattt gttaatgaga ttatatggac taatggtata
     1321 tttatactgt gctaaaagtt gcactattgt atgaggaatg ggaataaagg gtttgaaaaa
     1381 ataggtagaa gatttttcag atattcttac tgggatattt taattaatat attttaattg
     1441 cgatattttg ggaagaatag aatttattta taaatgaaaa taattaaaag gggttaaggt
     1501 tgtattagcc ccttttgctt atagagtttg gaaatggtat cttgatagca gttgtttcat
     1561 gcttattata ttttattaca tattttaaaa ttgaccttat tactcctaaa tcattgttat
     1621 atcaactttt aaaacacttt caagacaatc gcctattgaa aacaaagtta attaaaaacc
     1681 tacatatcag tttagtattc gtgtagatga ttatataaat ataaaagtat aagaaaggtg
     1741 agtatctgtg gttagttcat ttttaaatga tatctttaga acaattttag taagttgtat
     1801 ttttatagtt attttattag tctttagaat aactttattt aagattttca gtaaaaaatt
     1861 taattactat atatggctta ttgtgattat taagctatca ttaccattta tgaattatac
     1921 ttttatattt aatgagtcaa aatataatgg taatattaat aagataagtt taggcaattt
     1981 taatagtatt tctattgtat atagtactgt tttagtctct gtttggattg ctattacgat
     2041 attttattta gtttatactt tattgaaata tattaagctt aaaaatatga taaatgattt
     2101 gtcttatgat gttgaggatg ataagataaa aaatctttat gaaaatttat taaaagagct
     2161 taagataaat aaaaaaatac ggcttaaata ttcatatgaa gttgaaagtc cagtattttt
     2221 taattcgtgt gtcattttac caccaagtga ttatacatta aaagagttaa actggatatt
     2281 taggcatgag ttgatgcatt ttaagagtaa agacctttat attaagtatt tggtgatatt
     2341 tttaaaatgt gtttattggt ttaatccttt tgtgtatata atggagaagt ttatagattt
     2401 ggattgtgaa ttttattgtg atgaaagagt tttgaagaat tgtacgatag aagagaagca
     2461 ggattatgca ttgacaataa ttagtgcaat gagaaaaggt tcaaatagtt caaataaatt
     2521 tgtggctgga ttacataagg agtcagatat taaaaaaaga gtgtttagta tgtttaatga
     2581 aaagtacaga aatggtattt tgatagcagt tattttatgt ttattatctt ctattacata
     2641 ttttaaaatt gatttaatta ctactaaatc aatattttat ccactttcaa aacaaacacc
     2701 tattgaaaac agaactaatt caaaacctac atatcagatt aatgtttata tagataatta
     2761 aaaaatacaa agtttaacaa gagattaata taaataattt taataagtat ctttcacata
     2821 tgttaaattt actttaatta tattaaagcc aatgaattat tctttagaag ctccaatgcc
     2881 agtaaataca ggtaaagaaa aatattttac tacaattgca gattaaaaag tgttctaata
     2941 tttaaagaaa tacacaagtg taagaagggt aaaaatctgt tataatacat taaaaactaa
     3001 atttagaaaa atttacataa ttagaaaaat gatttgataa aaaataaagt taaatattaa
     3061 ctaagtaaat ttgaaaaaga aaaaatataa agtacaaatt taaaaggagg taactaatga
     3121 aaaagaagtt ggaagatatg actttagagg agttatggaa cttgtttcct atatttttga
     3181 ctactcacaa agaatgttgg aaaaattggt ttaaagaaga acaatataga ctaactggaa
     3241 tattgccaat gcaaaaaatt gttagaataa gtcatattgg aagtacttct gtgagtacga
     3301 tttatgccaa gcccattgtt gacatactga ttgagataat tcctgattat aatatgaaag
     3361 atattaaaga tttgattgag tcgaatggat atatttgtat gtctgagagc agtagtaggc
     3421 tttcacttaa taaagggtat acagtagatg ggtttgaaga acgagttttt catttgcata
     3481 taaggtatgt aggtgataat gatgagttgt tttttagaga ttacttaatt gaccacccag
     3541 atgttgcaaa ggagtatgaa aaacttaagt tggacctttg gaagaagtat gagcataatc
     3601 gtgatgctta tacagatagt aaactagatt ttgttaggga gtttactgaa aaggcaaagg
     3661 tgttgtattt taatagatat aattagaaat aggtacaatt agatatttat cttatatatc
     3721 gtattatatt ttttaagaaa gtaattaata gaaaaagggg ttgtttcaaa atggagagat
     3781 aaatcccttt ttcattgttg aaaggataaa aatatatttg atttcatgta aaaaagagtg
     3841 cctcatcaac taaaaagttt attttgagac acacacattt ttactaaggt aaataaaata
     3901 tcacatttga ttaaatttta agttaatgaa tatatgtgta aatatttaat tataagttat
     3961 tataatttta catagtatca aacaatctaa tttgtattat ataagataat ctaattttag
     4021 actaaaataa tctaatataa agaaataaag aaaattcatt ctcagtataa gagatatcca
     4081 attttccatt gtacttatta acaatttctc taatattctc aataccatat ccgtgagaat
     4141 tactatcttt tttatctgaa ataaatagtt cttgttttct attgatagga tttaccttgt
     4201 tgtttgtcaa taaatagcta aaatactcct tctcacaacg tgcctttaat accacttttc
     4261 tatttgacaa atcaggaatc ttcaaagatg cttctatagc gttatctaaa gaatttgaaa
     4321 aaatagtaca taaatctata ggttctatac ccaagtgatt gtctatatct atattaaagt
     4381 gcacatcaac accattttct tccaaacttg tatgacgaag atttaaaatt gagttaagag
     4441 cttggttttt gcaaaactgt ttgccactaa gagtacctat acctattttt aactgattaa
     4501 aatacttttt agctccagca atatcttcaa tttctaaata cgattcaatc atttgaaagt
     4561 gattattcat atcatgtcgc attttgcgta tttgaatttg ttgattttct agtaagttat
     4621 agtatttttc ttttagatga tttgcttgtt tttctatctt catctttaca ttttcaatca
     4681 atggctgtaa aagaagtatc acaccgatat ttgccagaat tgctgacaag atgattagat
     4741 aagtgccata aggtaagttg cctaagataa tattcttatt actacccata gggattaaat
     4801 tagtaggtgg cagaataata gcaattaata ttgataaaaa tgaagatata cataccatat
     4861 caataaaaat ccatatttta tttgataagt attgttttat attgaccaat ctattcttta
     4921 ggaaaatatg tattaaaaac caaaataata atttaattag agcagaaatt atctggacta
     4981 ttgaaagtat taaaatatac tcttttgagt gtataagttc ctcatatata gggttgttta
     5041 taaaaagaaa attaagccca attataatag gataaaatat catgacaact gatattatag
     5101 gaattaattt gcttttgcta catattataa gtagtataaa atatccgaat agtgtaaaca
     5161 aaatatttac ggggtcggaa agccatatta tcatttgact aataatagag tagcccaaaa
     5221 tcaatatgat tttgaaaatc caatgattcc taaagtctat aaaataggaa gtcaacgtat
     5281 aaagaaaaaa ggctatgaaa atattgatta atattagaat aaaatttatg cttgtagaga
     5341 ttaaagaata agtgcccatt taatatacct ttctattaat taagattctg gaaactaagt
     5401 tttaaaaaat taaatcttaa aaattaaaat tctggaaatt aaatttcaaa gaattgattt
     5461 tgaaaaataa aattctagag attgaacttc aaaaaattga gttttaaaag attgaaattt
     5521 taaaaattaa gtttcaaaaa attaaatttg aaaaaaaact ctaaaaacta agcttcaaaa
     5581 aattaaattt tgaaagattg aaattctaga aactaagctt taaaaaatta agtttgaaaa
     5641 aacaaaactc taaaaactaa gcttcaaaaa attaaatttt gaaagattga aattctagaa
     5701 attaatcttc aaaaaattaa atttgaaaaa acaaaactct aaaaactaag ctttaaaaag
     5761 ttaagtttca aaaataaaat tctagagatt gaacttcaaa aattaaggtt ctaacatata
     5821 ccttgtaaat tttcctacaa cctcttgata ttttttacgg cttataggaa ttttttcatt
     5881 ctgaactttg gcataagttt gagttatact atctacatgg tttagattga caatatacct
     5941 ttgatgcgta cgtataaaat tagagggaag aacagtttcc atatcattta attttccgta
     6001 gtattctaat gttttatcaa aggtaactac agttaatttt cttagctcac ttttaaagta
     6061 gaggatatct tttgtattaa ttttacaaag attagttcca atctgaactg taataaagtt
     6121 ttctttccat tgttctaatt tttttagtgc ttcttttaat actttacata ttttttcagt
     6181 tttataaggt tttaagatgt agttcaaagc tcctacatca tatccatgaa atacatagtc
     6241 agaaaaacct gtgacaaaga tgattatagt atcctttgaa tgttttcgta tagtttttgc
     6301 tatgtcaacg ccattacttt ttccaagttc aatatctaaa aaaataatat ctatgtaata
     6361 attttcgtgt tctagattgt atactagact ttctccattt gaatattctt ttatttggta
     6421 ggggtagccc cacaaatcca tttctcttgt aatgatttta ttcagcatgt ttctttgttg
     6481 tttttcatca tcacaaatac aaatcgaagt caatttttta tctcctttca aattttatat
     6541 ttatatagtt aagtatataa tatattttgc tacctagata tataaaatga aataacaatt
     6601 atatccagat agcaaaataa aattttttat attagtatac ttacacttaa aactattcta
     6661 ttaaacttat tctgaagtgc taatgatttt cttattaaaa aatctaacaa tctttcttat
     6721 tataaaatac aaatgctttt atta
//
LOCUS       DAMKMI010000175         6507 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMKMI010000175 DAMKMI010000000
VERSION     DAMKMI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.181"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..853)
                     /locus_tag="PC429_003389"
     CDS             complement(<1..853)
                     /locus_tag="PC429_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3848846.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNE"
     gene            994..2421
                     /locus_tag="PC429_003390"
     CDS             994..2421
                     /locus_tag="PC429_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF3848847.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIAKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFNWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2617..5211)
                     /gene="clpB"
                     /locus_tag="PC429_003391"
     CDS             complement(2617..5211)
                     /gene="clpB"
                     /locus_tag="PC429_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF3848848.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5248..5844)
                     /locus_tag="PC429_003392"
     CDS             complement(5248..5844)
                     /locus_tag="PC429_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848849.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5861..6265)
                     /locus_tag="PC429_003393"
     CDS             complement(5861..6265)
                     /locus_tag="PC429_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF3848850.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
BASE COUNT         2081 a         1052 c          695 g         2679 t
ORIGIN      
        1 gttcatttct atacttagtt ggagatatat tataattctt tttaaataag cgactaaaat
       61 aatttatgct atcaaagcca catctcatag caatttctgt catatttaaa tctgtttcct
      121 ttaaataata tatagcttta tcaagcctga ttccatttat aaaatcgatt gtagtcttac
      181 ctgttatttg cttaaatgtt ctacaaaagt gatgtgcact caagttcaca cgactagcaa
      241 gttcttttaa tgtaatttta tccatatatt tttcttctat caaatcaaag atacattcaa
      301 atttttctaa agaatacttt ctttttataa attcatcttc agaatatatt ttatcaatat
      361 gacttcttaa taagagtact attaattgat agatattagc ttttatagat aactcatatc
      421 caacttcttt attgtgatat tcatcaagta tagttgtaat acattttaaa atcagagaat
      481 cattttgaat taaattatta aatacaatat gatttaaagc caatggtgct aagtacttgg
      541 tttgtaacaa atcaacttga ttactaaaaa ggaatgtggg ttcaatacga atactataga
      601 attttaaatt atctgataag ctttctaaat aatgtaattc attactgttt gctattacaa
      661 tatttccagg cactacctta aaattatcca atccacattc aagatatgct ataccatcaa
      721 taaaataata tatttgtaga tgctcatgcc aatgtctttt aaagtttgct ccttttttaa
      781 tgtcatcctt taaaaaaata tctataaagt tttcttgacc tttcctgtta aaatatgtat
      841 ttttatcctt cattattccc ccctcatcaa aatagtgcta attataagca atatagttat
      901 tgtattcatt atttcatatc gctattatat aaaatgtaac tatcattgta aacattaaca
      961 taatgcagtc attataataa ggaggtaata aaaatgacaa actcttttaa aaaagatgat
     1021 aatagaaaaa aaatcacagt tttatttgta gtcattgcca tgacttttat ggcaacatta
     1081 gattctagta ttataaatgt tgctctccca gttcttgcaa gcaaactaaa tgtatctcta
     1141 gcatctattg aatgggtaat agcaagttac tcaattatta tttgttcaac actattattt
     1201 tttggaagac ttggagatat tattgctaaa tcaagagtat ttcaagtagg tactattctc
     1261 tttacatcag cttcattact atgtggatta agtaattctt taactttact tatagtttgc
     1321 cgctttattc aaggaatagg cgcttctgcc tacatggcaa acaatcatgg aattatcaca
     1381 gaactatttc caaaagaaag cagaggaaaa gccctaggga ttttagttac tgcagttgct
     1441 attggaaata tggttggacc atctgtggga ggatttattt tgtcaatatt taattggaat
     1501 gttatatttt ttataaatat tccaatcggt cttattgtaa tctttttaaa tacaaaattt
     1561 cttcctaata gtaaaaaatc ttcagaaaat atggataaaa ctggtgctat acttcaattt
     1621 ttaggaacaa cattattttt tagtgctcta attagtgctc aacaaacagg attactaaat
     1681 ccatatatat taattgcact attattatca attattttta ttatattatt tttgatatta
     1741 gaaaaaaaac atcctcaacc gcttcttgac cttgaaattt ttagaaattt taaattttca
     1801 ttgaatttaa tttgtgctct tacatcattc atttgtattg catcatcaag tattttaatt
     1861 ccattttatt tacaaagtac tatgaaactt ccccctattc aagcaggttt atttatgata
     1921 ctatcacctt tgattttagc cattttttca cctatatttg ggaatatatc agataaaatt
     1981 aagtcagaaa aaattatttt aattggtctt ctcgttatga gttttggatt ctttctaatg
     2041 tcaagattga aagaaagttc tgcccttatt ttatttgtaa tttatatatc gataatatca
     2101 ataggacaag ctatatttca acctgctaac aatgctttga taatgtcaag ctgttctaga
     2161 agtaagctag gagttgtagg aagcataaat tcccttgtaa gaaatttagg acaagttata
     2221 ggtattacta tttctacaac acttttatat aattttatga gtattaaagc tggatatagg
     2281 gtcaatgatt atgtgattaa taatgataaa atatttgtat ttggaatgag aaatgtttat
     2341 attattgtta cacttgtttg cttaataggt gcaatattaa ttggctttta tttatttaaa
     2401 tataataaaa atgaacaata gtttttataa ttatattcaa attatgatta tattataaga
     2461 aatttactaa aatacttcct ttgagttaaa tttaatttgg acatattgac ttaaaaatgt
     2521 tttattaatt tgataaaata tttcttttcc tatagtttat aaataaaaat cagctgagca
     2581 aaatatttag tatattatgc tcaactgatt cttttattat ttaactaaaa cttcaatatt
     2641 ttcatctttt ccatctataa ctatagtaca tccattatac acatctccag caataagttt
     2701 ctttgcaatt atagtttcta atgtatttcc tatatatctc ttaagaggtc ttgctccata
     2761 tacagggtca tatccttctc tcaccataac atcttttgct gaatttgtaa cttcaatagt
     2821 aatatctttt tcttttaatc tattttttaa atctttcata aatatatcta taattttctt
     2881 gatattttct tggtctaaag gcttaaacat tataatatca tctactctgt ttaaaaactc
     2941 aggtttaaat ctatgcttca tttcattcat tactaaattg ttagtagtct ctgttatatt
     3001 tcctcctgct tctaatagat aattacttcc tatattagaa gtcattatta taagagtatt
     3061 tttaaagtct actgtcttac ctttattatc agtaagtcta ccatcatcta atatctgtaa
     3121 gaacatgtta aatacatcct catgtgcttt ttctatctca tcaaatagta ttacagaata
     3181 aggagcacgt ctaactgctt ctgtaagttg accaccctct tcatatccca cgtaccctgg
     3241 aggaggtcca actagtcttg atacagcatg tttttccatg tattcagaca tatctattct
     3301 tattatatta tcttcactat cgaataaatt tctagcaaga gtttttgcaa gttcagtttt
     3361 accaacacct gtagggccta agaaaataaa tgaaccaatt ggttttcttt catcttttaa
     3421 tcctgctctt gcacgtatta ctgcatttga aactgctgta actgcttcat cttgtccaat
     3481 aactctttta tgaagttcat cttctagttt aagtaatttt tctctttctc cttcaactag
     3541 tttagttact ggtatacctg tccactttga tacaatgcta gatatttctt cttcagtaac
     3601 ttcttctttt aaaagagaat tttcagaacc atcattcata ttttcttcgt actgctttat
     3661 ttgttcttct aacttaggta tttcaccata tttaacctct gctgctttgt tgaaatcata
     3721 ttctctttca tacttttcag ctttaccttt agcttcgtct aattgagcct ttaagttttt
     3781 aatatctaat atttgactct tttctttttc atattttgct gtcatttcat catttttaga
     3841 ttttaactct gctatttctt tttgaatatc atccaatctt gatttacttt tatcatcatt
     3901 ttctttaagt aatgcttctc tttcagtttc taatgtaaat aactttcttc taacaacatc
     3961 taattctgtt ggcaatgaat ctatttcact acgaatcata gctccagctt catcaattaa
     4021 atcaattgct ttatctggta aaaatctatc ttgtatatat ctatgagaaa gttttgctgc
     4081 tgctacaata gcattatcat gtattcttac tccatgatgt atttcaaatc tttcttttaa
     4141 acctctaagt atagatattg tatcttcaac agtaggttct tctgcaatta caggctgaaa
     4201 acgtctttca agagccttat ctttttctat atactttcta tattcatcaa aagtagttgc
     4261 accaatacag ttcaattcac ctcttgcaag cataggttta ataagattac ctgcatccat
     4321 tgaaccttct gttttaccag ctccaacaat agtatgaatc tcatctataa acagtataat
     4381 ttttccatca gaactttgaa cttcttttaa aactgccttt aatctttctt caaattcacc
     4441 tctatatttt gcaccagcaa ttaaagctcc catatcaagt gagaatacaa tcttatcttt
     4501 taaaccttct gggacatctc ctcttactat tctctctgct aacccttcta caattgcagt
     4561 tttacctaca cctggctcac caatcaatac aggattattt tttgttcttc ttgataaaat
     4621 tcttataatc cttcttattt cttcatctct cccaattaca gggtctaatt tatttttctt
     4681 ggctaaatct actaagtttg ttccatatct agcaagtgca tcatatgtgc cttctgggtc
     4741 ttgtgtttct actctttgac tacctctcac ttgtgataaa atatttaaaa agtcattctt
     4801 atttatatta tattgtttta atattttacc aacagctgat tttgattctg tttccatcat
     4861 tgcaagcata acatgttcta cacttatata tgaatcttta aattctttag aaatactctc
     4921 tgcctttata agcacctcat ttatttttct agtagctgat actccttgtg agtcagcacc
     4981 ttctccatat acttttggaa gcttatcaat ttcaaagttt acagtatttc tcaaagaatc
     5041 aattgatata ttcatttttt cgattatatt tggtatcaat ccatcctctt gattaataag
     5101 tgctgagaaa agatgaatta catctacttg ttggttatga ttttttactg cttcgttata
     5161 agcctcattt aaactttttt gaactcttaa agtcattttt tctacgtcca tataaatccc
     5221 tccattttaa tattatatcc aaataaatta aattctctca tttataatta ttgattttct
     5281 aagctctaaa agactatcaa gtaaattctc gtttttttgt atatcgaaac tcaatgttaa
     5341 attaaatgtt ccatctgaat taaaaaaatc gaatacttct tttgattttg ctattaaata
     5401 caagtcttca tctataagtc ttttttcacc tattaataaa tactgacatt tatctataaa
     5461 actcttttta aaatcaatag tgtatagatt ttctaaaata ttatattgat aaatagcaat
     5521 attattaaac acttttaata tgtttgaatt tacaaaattg taaatttgat tattgttata
     5581 cagtgatatt tgttcgtata tatattccct aaataaagga tgaatgtttt gcttaagttt
     5641 taaagtaact tcagctggta tttcaaaata aagcttccag tacttagagt tatttttttc
     5701 taaaataaaa gctactttgt tcaaactggt agaactaatc tcctttagtg tacaattaaa
     5761 attatagtgt ctcaaactag ttttttcatt ctcaaataca tagtcaaacc actgtagaac
     5821 atttctatca agttttaaac tcatataaaa accttcttcc ttatttacta accttattag
     5881 gtctttttat ctgattatat tctgctaata aactttcttt atcagctcta ctacctttat
     5941 atgctatatg tggatttatc atatactcac catttttcac tctttgtaaa aaatccattt
     6001 ttttcagctc cgtaaacaat gaagataaag aagtttttgg atattcaaat tcacaagata
     6061 attcatataa tgtttttcta atcttattat ttttatccat agagtttatt aattttaatg
     6121 gaattaaatc cctcttagta tacattcctt ctactacttt aatataattc ttaaaacaaa
     6181 cattcatata cttactttta ttcataacta aacttttgct ctctgtatat tcctgattta
     6241 aatccttttt taatgtctta tccaaattaa atatctcttc tcactctcct tatctctaat
     6301 tgataatata aatatattac aaaaaaatat gtatgtcaaa actgaaaatg caaaaagttc
     6361 accttataag taaactttta tttagctata agctaaactt tattattttt ttaatttagt
     6421 atttccaatt tattggcagg atttttgaac aatatttata aattttttaa aaaaatttaa
     6481 atctatttaa tatggcagac tttcata
//
LOCUS       DAMKMI010000176         6408 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMKMI010000176 DAMKMI010000000
VERSION     DAMKMI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.182"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            135..3314
                     /locus_tag="PC429_003394"
     CDS             135..3314
                     /locus_tag="PC429_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3848851.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPREYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3599..4363)
                     /locus_tag="PC429_003395"
     CDS             complement(3599..4363)
                     /locus_tag="PC429_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3848852.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4519..4752)
                     /locus_tag="PC429_003396"
     CDS             complement(4519..4752)
                     /locus_tag="PC429_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848853.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4826..6319)
                     /locus_tag="PC429_003397"
     CDS             complement(4826..6319)
                     /locus_tag="PC429_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3848854.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYNSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2379 a          905 c          740 g         2384 t
ORIGIN      
        1 tagtaaattg ttatttacac atatattgtg atagataaaa gctattcata tctgccttaa
       61 ttttagtctt aaatttagag tgtaaattga atgcatttag attataatat atacagtgag
      121 gtaaggtgaa taatatgaaa atagatatga tatataaact aataagaaga aatacagaac
      181 catcaagatg gtcaagaggt gtaaaatatt atactaatca attagttgat tatatagaca
      241 tagatattga tgacctacaa aataacctgt tgacatttcg tggtggagtc aattctgaag
      301 ataattatag cttttatttt aattcaataa ctatagatat aaagcacttt aagataattg
      361 aaaatgattg tagttgcgct gactgtcaat caaagggcaa aagtattttt atatgtaagc
      421 atatagttgc tattgtgata aaaagcttaa aacaaataaa aaataaaaaa gatgttcctc
      481 ctattctaat agatatatta ggtgcagata aggataagct ttctaataca aattctaaaa
      541 ccagcttaga tttaagcaag caattacttt ctttgttaga caatactaaa agagaaaggg
      601 ttaatttaga tgtattgata aatgtggatt caaataaagc aattgctgaa tttaaaatag
      661 gaattgataa aatgtacatt ttaaaaaaca ttactgaatt tgcatattca aaacttaatg
      721 gatatgaatt agattttggg aagaaattta cttacaaccc caacactcat tattttgctg
      781 atgaagatga gaagttagta gatattatag aagaatatgg gacaaatctt tcatacaact
      841 tttcttcatc acattcaaaa aaatttatgg agataaatgc aagctcttta aaacaatttt
      901 taaatgctat taagtacaag gattttaccc ttattttttc taaagaggaa tatcatccaa
      961 agatattaaa tgatactcta cctgtaaggt ttgattttaa aaatatagaa gataaaatag
     1021 tcttatattc taaagattca ctacctgttc cacttactaa aaaaaatgat gtacttttat
     1081 atgatggcaa tatctattta ttatcacata aaaaagctca tgattattca aaaatatatc
     1141 aaatattatc aaagactaaa gagttaaaat ttgataaaga ggattctaag gatgttttag
     1201 gattgctagt tcctagatta aaatctatat ctcaagaagt tcatttagat gataacataa
     1261 aaaataatat aacaaaagat tttaaatctg agttctactt tgatatgatt gatggcaaca
     1321 tatgttgtga tgtaaagtat atatatgatg atgaagataa aaaatttgtg ctccccaaca
     1381 tacaaaagga agctactatt gaaaataaac ttacttcttc acaatttaca aaggaaaata
     1441 atcattatgt gtttaaagga actgaccatg acctatttac atttttagat ttacaattag
     1501 aatctctaaa ggaatttgga gatatatatt attctgagaa gtttaaattg aaaaaaatat
     1561 ataacgcttc atcaattcaa gcttcaatca atcagactaa ccagaattac ctagaattta
     1621 attttaatat atctgatata aatccaagag agtacaagga tattttgaaa gcttttcaag
     1681 aaaaacgtac tttttataaa cttaaagatg gaaactttat agatttatca gaaagagaaa
     1741 caaaagattt ttttgaatta gttgaaaatc tagatataat ggataaatca aaaaataata
     1801 aaattcataa aaataaagct ctatatataa atgatgtaat acgtagtaaa aatttaaagt
     1861 ttatagatgg taaaaaagaa ctagacagta tatgtgaaaa atttagaaat tttgatagca
     1921 taaatttaga aattcccgca aatttaaatg caaaccttcg tgactatcaa ataaatggcc
     1981 tcaattggtt taaagtttta gattattata aatttggtgg tatattagct gatgaaatgg
     2041 gtcttggtaa aacaatacag actattgcat ttttattatc attatctaat aaaaaaagtc
     2101 ttattgttac accgacatca ctcatttata attggaaaaa tgaatttgaa aagtttgctc
     2161 cagatataaa agttttattg atacatggaa ataaaagaga tagagaaaaa tgttttatgg
     2221 aattagaaaa ttttgatgta atacttacaa catatggtac tcttagaaat gatttagaaa
     2281 aatatagtga aataaagttt gattattgta tattagatga agctcaaaat ataaaaaatc
     2341 cagtagcttt agtaactgag tctgtaaaaa gtataaatgc agaaaataaa tttgcactca
     2401 ctggtacacc tatggaaaat aatctactag aattatggtc tatatttgat tttataatgc
     2461 ctggctatct atatagtaaa gcaaagtttc aggaattgtt tataaataaa gaagataatg
     2521 ttaaaaacct gaaaaaattg ataaaacctt ttattcttag aagaagtaaa aaacaagtta
     2581 tgaaagaact tccagataag attgaaaaaa acttttttgt tgaattgaat aaagagcaga
     2641 aaaagatata tagtgtatat tcaaaagata tacaagataa aatgaaagat aaaaatctaa
     2701 agaaagataa aatagttata ttttcttatt taactaaact aagacaactt tgtttagacc
     2761 caagtatagt agtaaaagat tacaataaaa aaagttcaaa aattgaaaca tgtttagaaa
     2821 tacttaggga tagtataaac gaaaaccaca aaatattatt gttttcacaa tttacttctg
     2881 tgcttaagaa cataagtaag gaacttgata agtataaaat taaataccat tatatagatg
     2941 gtaaaactaa tgccaaagaa agacttgaac ttgtagatga gttcaataat agtatggata
     3001 aaaaagtttt tttaatatct ttaaaagcag gcggaactgg tttaaatctt acatctgctg
     3061 acatggtaat tcactttgac ccatggtgga atccatcagt agaaaatcaa gctagtgata
     3121 gagctcatag atttggtcaa aaaaattcag tacaagttat aaaattgatt gctaaaggga
     3181 ctatagaaga aaaaataata aaattacaag aaagtaaaaa agagcttata aatcaattta
     3241 taaatggaga actttctaat gaaggtgtat taaaaagtct atctgatgag gagataataa
     3301 acttatttaa ctaaattatt taatcctaca gcttaactat ataaatttgt tctaacattt
     3361 atatagttaa gctaaaatat ctgaataaat aaaatttaat attgttattt aatcccaact
     3421 ttacaatcta atgatatctc tttctataca tattgcatat acaaatgtaa taacttgact
     3481 attagaatat tttgttttaa caatactatt tcatcataat aatgaagtat atgtacactc
     3541 acttttgtaa gttttgcaat ccattttaca gttttcatta ttataaattt tggttaaatt
     3601 attcctctaa aaatttcttt gctacatttg aaaaatctaa tccattcgat aaaattgcag
     3661 gatgaccacc tttatcaaac aaatacattt taccattttt aatcttttca agaagtaatg
     3721 aaaaagtatt ttcaaaaaaa tcactagata ttagagatac aaactcatct tctctacttc
     3781 ctgttaaaag cacctctggt tgcatagttt ctagaggctt atgaaaaaat tttcctatag
     3841 ttttataatg ttcaaaaata gcttctgtat cattatctac aactttttcc cagttttctc
     3901 cttgattata aatataaaaa gcttttgcac catcatcttg cttagatgct tcacgctcta
     3961 ttttcacatt ttgtacaaat gactctagag gaacttctcc ttcaaaacta tctgcaatta
     4021 ccttattcac taaatcaggt ctctcaagtg caacatttaa tgcaacaaga gctcctccac
     4081 tactaccaat tatgtttact tttttatagt tcattgcttc taaaaaacat atcacttgca
     4141 tagcctcatc aaaccataag tcagcagaaa atttatcgac catttgagat tttccatgac
     4201 ctacaaaatc aattaatatt actttatatt catctttata aaaatcgatt atatcattaa
     4261 acatcttgga agaggctgta tttccatgta aaaaaagtaa tggtgtacca tccccaattt
     4321 cattatagta gcactgtttg tcgttatagt taaaatatgc catatatttc ccccctttaa
     4381 tatccctata ggtatatatt tattacttat atttaaaatg actattccat tagccactta
     4441 aaaatagtat aacttagaat gtagataaaa tcaacattcc ttgctaattt atctattgtt
     4501 tgtctaattt attataattt aacttctgta aaatttcttt tttgtacgaa aatattcctt
     4561 ccatttacaa caatcattca aatgaagtat aacatgacga gtaggaggca taagcaaaat
     4621 tccagcaata tctttattcc cccaaaagtc caacaaccaa atagaatctt catgctttat
     4681 tactcctcca gtcaccaaaa tcctttctaa gtcaattgga gagacctttc gtttcatatc
     4741 ttcagctttc aaatccttaa caacttctat tgtgtttttt gcaacttcat ttcgataaca
     4801 aagcaattca tcaatattga ctaaattatc gtatatctct tttcacttta accatttttt
     4861 tctttatagg tatctcctta tattttttta atatcaaatc tcccttataa tttaatatat
     4921 ctacatatga acataaatct tgaacttcaa ttacacctat atcatcatta gttatacctt
     4981 ttatattact aaatgcacca actatatcta aaactcttat tttcttcttc ttaccagcat
     5041 ttaaatataa tttaacaact tcactttcta tatttagttt tatatcttta ctattgtaag
     5101 aatttttatc aacttttttt ctctttgata ctttttttag gattcctttt gaatattctt
     5161 caaacaaaat tttgccatgt gtaattctac ttttttctat atcttttaat tcatttattt
     5221 tatatcctat atacgtttct atattctcta tatatttttc atccttttca gttactatag
     5281 taattgcctt tccactattt ccttttctcc cagttctacc tattctatgt atatagtttt
     5341 ctttatcttg tggcacatca taatttatta ctaaagaaat atcatcaata tgtattcctc
     5401 tggacgctac atcactacta actaatacat taaatttttg gtttttaaaa tccttaatta
     5461 caaaaattct tctttcttgt gataaatctg catgtaactc tcttattaaa aaaccttctt
     5521 tactcatctt gttatataat ttagatactt tctctttggt attacaaaat attatcacac
     5581 tctttggatt ttcgctgtat attaattctt ttaaaaggat gtacttctct cttccatttg
     5641 ccttgattat tttgtcatct atttgtcttg ttttttgatt agtatcgctc tcattttctt
     5701 ctatatttat aacactgtaa tctagcatat atttttcaca tatatatttt atctcttcat
     5761 ctattgtagc tgaaaaaaga cttactattt tctccttagg taaatttaat aatatttttt
     5821 ccatttgttc aacaaaacct ttattaaaca ttttatctgc ctcatctatt actaaatact
     5881 ttacattttc caacttaata gaacctctat ttatatggtc aagtattctg cctggagtag
     5941 ctacaacaat atgaaccctt tgtttcaatt cagctatttg gtcttttata gattgtttac
     6001 caaatatagc actacatctc accttcttta gtctccctat atctgatatc tcatctttta
     6061 cttgcaaggc taattctctt gttggaacca ctataagagc ttgaatatta ttgtaatcta
     6121 catttatatt ctcgcataaa ggtattgcaa aacttgctgt ctttccactt cctgtttttg
     6181 acctaactac taagttttga ccttttaata attttggaat aacttctctt tgtactctag
     6241 aaggtatatt atatcctaaa ctcttcaatg acttaagtat cttttcattt aacttaaatt
     6301 tttcaaatgt atatttcata tattaccacc ttattatatt tatcttatct atatattttt
     6361 ctacattata ttattataat acaaggttct actattttta tattattt
//
LOCUS       DAMKMI010000177         6329 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMKMI010000177 DAMKMI010000000
VERSION     DAMKMI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.183"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            293..1237
                     /locus_tag="PC429_003398"
     CDS             293..1237
                     /locus_tag="PC429_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF3848855.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1259..1885
                     /locus_tag="PC429_003399"
     CDS             1259..1885
                     /locus_tag="PC429_003399"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF3848856.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1919..2968
                     /gene="hisC"
                     /locus_tag="PC429_003400"
     CDS             1919..2968
                     /gene="hisC"
                     /locus_tag="PC429_003400"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF3848857.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2971..3549
                     /gene="hisB"
                     /locus_tag="PC429_003401"
     CDS             2971..3549
                     /gene="hisB"
                     /locus_tag="PC429_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF3848858.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3551..4156
                     /gene="hisH"
                     /locus_tag="PC429_003402"
     CDS             3551..4156
                     /gene="hisH"
                     /locus_tag="PC429_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF3848859.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4153..4875
                     /gene="hisA"
                     /locus_tag="PC429_003403"
     CDS             4153..4875
                     /gene="hisA"
                     /locus_tag="PC429_003403"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF3848860.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4857..5615
                     /gene="hisF"
                     /locus_tag="PC429_003404"
     CDS             4857..5615
                     /gene="hisF"
                     /locus_tag="PC429_003404"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF3848861.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5643..6311
                     /locus_tag="PC429_003405"
     CDS             5643..6311
                     /locus_tag="PC429_003405"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF3848862.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2585 a          537 c         1253 g         1954 t
ORIGIN      
        1 gtaaatttag aatattaata aaaattattt gggaggaaat tatcatggtg agattaaata
       61 tagtaaaatt aaatacaaaa ttaaatcatc accatcatca tagattaaag ggattgtaat
      121 atgaaattaa ttcatagaca caagccctgt ttttagtgta attggtcttg tatctatgat
      181 gggaagtaat tttaagagaa aattatagcc atataggtat aaagcctatg tggctttttt
      241 attgttttaa atttgcaaat ataagagtaa atttaatgaa gagggtgaga atgtgaaatt
      301 tttgaggatt gaagatgagg ttatttattc taaaaaaaga tataaattaa aaagggaaat
      361 agataaactt tttttggatg aagaatattt tcaaatagaa ccacaattat ttgaagaata
      421 tgatgaattt acaactataa ataataaaat tccagaagaa tctatggtaa aggttgtaaa
      481 tggaaaagta atggtactta gagctgatat aactacaagt ataataaaaa gccttgtacc
      541 aagatgggaa gatggtttaa agcttaagct tttttataat tcatcaatat ataaaaataa
      601 aaatacagtt ggaataaaag agataagaca aataggttgt gagtatctag gagaagcctc
      661 tgtagaagca gatagagaag tagtgaaatt agctcttaag atacttgaaa aatacaataa
      721 taattttatt ctagaggttg ggagtagcaa gtatattcat ggtttgttag aagaattaaa
      781 tttaaataag aattgtgaaa atcaaattaa aaatttacta tatacgaaga acactcatga
      841 actaaaagta tatattgaag aattaaaaat taagcgtgaa gtaaaagagt tattatcaaa
      901 tattttaagt ttacaaggga atttatataa tgttattgaa aaatcaaaca agttctattg
      961 taataataag atgaagcaag ctcttgaaga attaaagcaa gtaaataact tgattgagga
     1021 atgtaatttt ttagataagg ctagatttga tttatcaatg attactatgc ttgactatta
     1081 tgaaggaatt atgtttagag gatactaccc aaactcatat aaagaaattc ttagtggtgg
     1141 aagatatgat tctttaacta aagagtatgg aaaagaaatt ccagccattg gatttacact
     1201 aagtgttgat gagttaatga agtatgtata taagtaaagt ttagaaggag ggattgcttt
     1261 ggattatttg actatagcac tagcaaaagg acgaattgaa ggggaatcat ttaaaaaatt
     1321 taaaaaaatg ggtctaggag acagtataga cacagatacg agaaaactaa tttttaaaga
     1381 tgaagaaaat aaaattatat atatacatgt aaagccatca gatgtagtta catatgttga
     1441 aaaaggagtc gctgacctcg gtatagcagg taaagatact attttagaga atgagacaga
     1501 tgtatatgaa atatatgatt taggttttgg aaaatgtaaa tttgctgtag caggattaaa
     1561 gggagatagc atatatagag aagatgaata tttaaaagtg gcaacaaagt atcccaatat
     1621 agcaaaaaag tattttaaag agaaaggtca aaaaattgaa ataataaaat taaatggttc
     1681 tgtagaacta gctccaatag tagggttgtc agatgtaatt gtagatatag ttgagactgg
     1741 caatacactt aaagcaaatg gtcttgaaat attagaagac atatgtaata taagtgctag
     1801 aattatatca aatagagcta gctatagatt taaatatgaa caaattcaga atataattcg
     1861 tttatttgaa gaacttgata attaaaatat aattgagctt tataaggagg gatttaagtt
     1921 gagagaaaaa gaaagcataa gagagttaaa gggatatgaa ccaaatcacg taaactgtaa
     1981 agtaaaactt gatgcaaatg aaggaagtaa gaggttgttt aaatacctta ttaaagaaat
     2041 ttcagatagt gatatagatt taaatttata tcctgaagat tcttatagtg atttgaaaga
     2101 atctataata gattacataa atatttctgg agtaaataag aaaaatcttc tagtaggaaa
     2161 cggttcaagt gaaataattg atttaataat acatactttt gtagataaag atgaagtaat
     2221 attgagtttc tctccaagtt ttagtatgta ttctatatat agtcagataa atgggtctaa
     2281 atttataggt gttgaaagtg atgaaaattt agttataaat atagataatg tgattgaaaa
     2341 agtaaaggag aataatccta aaatagtaat tgtatgtaat cctaataatc caactggaac
     2401 aatattaaag agggaagaga taataaagct tttagattca actaatagtc tagtggtttt
     2461 agatgaagca tatatggatt ttggagaaga aagcatgtta agtgatgttt ttaagtatga
     2521 taatctaata gttcttagaa ctttatcaaa ggcatttgga ttggctggaa taagaacagg
     2581 atatatgtta tctaatagta gtttaataaa ttctgttgag aaagtaagac ctccatataa
     2641 tttaaattca ttatcagact ttattgctac aagagcactt agaaataaag atgtggtaaa
     2701 ggcttatata aaggaagtaa aagaggaaag agaagtttta tataaagaaa tgattggtat
     2761 gggaattaaa gcatataaat cacaagcaaa ttttatatta ttttattctg aaatcgaaaa
     2821 tttatcacaa aaacttattg atagaggtgt tttaataaga aaatttggtg gaaagttaga
     2881 aaattattac agagttacaa ttggtgataa agaagaaaat tctatgtttg ttggagctat
     2941 aagagatatt ttgaaaaagg agaaataggt atgagaattt ggaaggtaga aagaaatact
     3001 cttgaaacac agattttagt agaattaaat attgatggtt ctggtaaagc tgaaatagat
     3061 actggaatag gatttttaga ccatatgctt accttaatgt catttcatgg caagttcgat
     3121 ttaaaagtga tttgtaaggg tgatacatat gtagatgacc accatagtgt agaagatata
     3181 ggtatagcta taggtgaagc atttaaaaat gctctaggtg acaaaaaagg aatacgaaga
     3241 tattctaata tctatatacc tatggatgaa tctctatcaa tggtagcaat agatataagc
     3301 aatagacctt atcttgtatt taatgctaaa tttgataccc aaatgattgg tagtatgagt
     3361 actcaatgct ttaaagaatt ttttagagct tttgtaaatg aatcgagagt aacattacat
     3421 atcaatctct tatatgggga aaatgaccat cataagatag aaagtatttt taaagcattt
     3481 gcaagagctt taaaagaggg tagtgaaata gtttctaatg agatagcatc ttcaaagggg
     3541 gttttgtaga atgaatataa tagtagacta tggtcttgga aatatagatt ctgtgtctag
     3601 gggttttaga aaagctggaa tagaaactaa aatttctagt gatatagatg aaataaaaca
     3661 ggcagattca cttatacttc caggtgttgg agcttttaga gattctataa gtgccttaga
     3721 taaactagga ttgataccaa taataaagga gcatgtttct aagggtaaat ttatgatagg
     3781 tatatgtcta ggaatgcaat tactttatga aaaaagttat gagtatggag aatatgaagg
     3841 attaggactt ataaaaggaa gtattgataa actggatata agtttaaaag tgccacatat
     3901 gggatggaat aatctaaaat ttaataaggc aaatgatgat atacttaaat atataaatga
     3961 agatgattat gtatacttcg ttcactcata ttatgcaaat tcatcaaatg aagaactaat
     4021 tgcattttca gaatatgaaa agaaaatccc tgctattgtt agaaaaggca atgtatatgg
     4081 tatacaattt catcctgaaa aaagtggaga agtagggtta aatatattaa gagcatatgg
     4141 ggagatgata aaatgataat atttccagca atagatataa aagataataa gtgtgtaaga
     4201 cttacacaag gagaatttga caaagtaaat gtatattatg ataatccatt agaagtagca
     4261 tataaatgga aaaatgaagg agcagaatat atacatatag tagatttaaa tggtgctaga
     4321 agtgaatttg gtgtaaatac aaaaataatt gaggacatag caaacaatat agatatacct
     4381 attcaagttg gcggtggagt tagggataag gaaaaagtca aaagtttaat aaatgctggt
     4441 gttactagag taatattagg tagtatagcg attgaaaatt tgaatttagt tgaagaattg
     4501 gtcaatgaat ataaagagaa aattgtagtt tctatagatg ctaaagatgg aaaagtagca
     4561 gttaggggat gggaagttgt aagcaatgta gactcattaa cattatgcaa acaacttgaa
     4621 aagataggtg tacaaactat tgtttataca gatatatcaa aagatgggat gttgcaaggt
     4681 ccaaattttg acatatatga aagaatagca aaggaaacat cactaaatgt tattgcttct
     4741 ggaggcgtaa cttctataga agacgtaaaa agactaaaag ctatgaactt atatggtgca
     4801 ataataggta aagcgcttta tgataaaaag atagacttta aggaggcaca acaattatgc
     4861 ttactaggag aataatacct tgtctagatg tgagaaatgg aagagtcgta aagggaaaaa
     4921 aatttaaaga tatagtagat gttgatagtc cagaagtact tggaaaattt tatagtgatt
     4981 gtggtgcaga tgaacttgta ttctatgata taactgcatc aaatgaagag agaaagacat
     5041 ctttagaatt tgtgacaaaa gttgcagaaa atataaatat accattttgt gttggtggag
     5101 gagtaaataa attagaagat ttcactgata ttttaagaaa aggtgctgat aaagtttcta
     5161 taaattcatc agcagtcaaa aatcctgaat taataaggga agcttcatta aaatttggag
     5221 cacaatgtgt tgttttatcg atagatgcaa agaaaaatga agagggttca tggagcgtat
     5281 acgtaaaagg tggaagagaa aaaactaatc tggatgcaat tgaatgggct gttaaaggtg
     5341 tagaacttgg agcaggagaa atcgttgtaa atagtatgga tgaagatgga atgaaaaatg
     5401 gatatgatat agaattatta tcaaagataa cttctttagt aaatgtacct gtcatagcat
     5461 ctggaggcgc tggaaaaaag gaagattttt atgaggcggt taataaatcc aatgtagatg
     5521 gaattttagc agcatctgtt tttcattttg gcgagataaa aataaacgat ttaaagaaat
     5581 atttaaaaga catgggtgta gaagtaagat tataagttaa atactaaaag gagattagaa
     5641 atatggataa taagtgtaac aatgtataca gtgatgaagt agagaaattt ataagaagta
     5701 taaagtttga tgataaaggg ttagtccctg ttgttgttca agaggtagtt agcaaagatg
     5761 tactgatgtt agcatatatg aataaagatg ctataaagaa aaccttaaaa gataaagtag
     5821 cttgctattt tagtagaagt agacaagaac tttgggttaa aggtgagaca tctggtaata
     5881 ctcaaaaagt agttaagatg tcttatgatt gtgatgtaga tactatacta ctttttgttg
     5941 aacaaactgg tgtagcttgt catacaggca attattcttg tttttacaga gacttgtttg
     6001 atgatagttc aaaaatggag cttgaagtac aaacaaatat attgaaagaa ttatatgatt
     6061 taatcaatga aagaaaaaat aatccagttg aaggttctta tactaattat ctttttgaaa
     6121 aaggaataga taagattctt aaaaaagtag gtgaagagag tagtgaggta attatagcat
     6181 caaaaaatac cgataaaagt gaacttattt acgaaattag cgatttagta tatcatacat
     6241 tggttttaat gatagaaaaa ggtgttgaaa ttgatgaaat aaaaaaagaa ttacttaaaa
     6301 gaagaaaata gaataagata gattaatgg
//
LOCUS       DAMKMI010000178         6208 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMKMI010000178 DAMKMI010000000
VERSION     DAMKMI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.184"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            132..677
                     /locus_tag="PC429_003406"
     CDS             132..677
                     /locus_tag="PC429_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF3848863.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
     gene            690..1703
                     /locus_tag="PC429_003407"
     CDS             690..1703
                     /locus_tag="PC429_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF3848864.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            1925..3898
                     /locus_tag="PC429_003408"
     CDS             1925..3898
                     /locus_tag="PC429_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF3848865.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            3967..4326
                     /locus_tag="PC429_003409"
     CDS             3967..4326
                     /locus_tag="PC429_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848866.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            4361..4717
                     /locus_tag="PC429_003410"
     CDS             4361..4717
                     /locus_tag="PC429_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF3848867.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            4754..6046
                     /locus_tag="PC429_003411"
     CDS             4754..6046
                     /locus_tag="PC429_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF3848868.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
BASE COUNT         2454 a          579 c         1181 g         1994 t
ORIGIN      
        1 tattaaaatg atattaataa aaaaacattt acaatattag catatagtac agaaaaaaga
       61 tgtaatttgt aaaatttatg gtaagcagta aaattaaaaa tttaaagata ttatagggag
      121 gttataattt tatggaaaac gttattacag gtctaagtaa aggtgcagag tggtttatcg
      181 gtttgtttca gaaaggggga gaacaattta taggtcttgt ttcaggtact cttccaacgc
      241 taattgtatt aatggtagct attaactctt tgattaagat aattggtgag gaaagagtta
      301 ataattgggc ttctaagcta ggaaagaact cttttacaag atatattcta cttccattag
      361 tatcagtatt tttcttggga aatcctatgt gctatacatt tggtagattt ttaaaagaag
      421 agcacaaagc aggattttat gatgcagcag tttcatttgt acatccaatt acaggtcttt
      481 ttccacatgc aaatgcagga gaactttttg tatggttggg aatatctgca ggtttgacta
      541 ctttaggtaa agaaactact acattggcat tgtggtattt tatagttggt ctaattgtaa
      601 tttttataag aggtatagta acagaaaaaa tgtatgcttt tctaacaaga aaaaatagag
      661 caacaaataa ggcttaggag gtagataaaa tggaggagaa taaaatatta aaaatagaaa
      721 agggaacttc aggctgggga ggtcctttgt atataaaaaa agaagggaat agaaataaga
      781 tactgtcaat gactgctggt ggtatccatg aagtaacatt aaaaataaaa gaactactgg
      841 gttgtgaaat tgtagatgga tttaaaactg gagtttctga tgaagaagta gcagtagtta
      901 ttattgattg tggtggaact gctagatgtg gagtttatcc aaagaaaaaa atacctacaa
      961 taaatgtaaa tccagttgga aaaacaggtc ctttagcaaa atttataaca gaagaatatt
     1021 atgtatcaga tgttaatcct aactgtataa gtgttgtaga tggggaagat atgccacaaa
     1081 aatctcaaga aaataaaagc gaaaataaga gttctattag aaagcctgat aattatgatg
     1141 aggttaagtc aaaagcacaa ggtgaatatg ctaaaaagaa cataatactt tctattggtc
     1201 aaggggcagg gcaagtagta tcaaaatttt atgatgcagg tcgagatact atccaaatgg
     1261 taatgaataa cgtaatacca tttatggcat ttgtaagtat gcttatggga attattttag
     1321 catctggact gggagactgg attgcaagag ttatatctcc acttgcaggt aatattggtg
     1381 gattattgat tatatcagta atttgcacac ttccattctt atcaccaata ctaggacctg
     1441 gtgcagttat agctcaagta gtagggactc ttgtaggtac acaaattggt cttggtgcaa
     1501 taccagcata tttagcactt ccagctttat ttgcaataaa tgggcaagct ggatgtgatt
     1561 ttgtaccagt aggattaagt ttgggagaag cagaaccaga aactgtagag tatggtgttc
     1621 cagctttatt ttactctcgt ttaataactg gtccaatagc tgttattatt gcatatggag
     1681 ttgcagtatt tgcattgaga taatatttaa tgtcaatata aggcttggaa ttaattaata
     1741 aaattgtaaa tataaattta agctagtcaa gattttttgt taatgaaaga ctagctttta
     1801 tactattaat tagctgtaat tatcgagtta gattaatact aaattttcct ttattaaagt
     1861 gaaattttat gtattaattt ggtttcaaat ttgtactaga atgaaatcaa ggaggagata
     1921 caaaatgagt gtaactgaga aaattccttc tattgaccgc aagatattta agatattgat
     1981 tatgtgtagt aaaaaagagt ttgtgagtat taactcaata gcaaatgaat tgaatgtcac
     2041 aactagaagt gtaaggacgt atataaaaca attgaataaa gatttaggta atgatatagc
     2101 agtaatcaaa tatataaagg gtcaaggcta taaactagaa ataaaagatg agcagatact
     2161 caataagatt attgatatga atagaaaaaa tgtattttca ttaaattcaa aagaagatag
     2221 agtagagttt atactaaatt acttgataga actagatgga tttattacac ttgatagttt
     2281 agctgatgag atgtgtgtag gaagaactac tttagtaaat gatttccagt atgtagaaaa
     2341 agttttggct tcatataatt taaacttgat taaaaaacag aatacaggta tgaaattaaa
     2401 tggaaatgaa cttgatatta gactgtttat attaaatcag ttgtataaaa actcaaggaa
     2461 agatttcagt aattcaaagt attttaaagg aatcaaaaaa gaagaaatca taaatttaga
     2521 agaaaaacta ttgactttat ttaaaaaaaa taatttttat gttacagatg aaatgttaag
     2581 agaagttatt aactacatag tagttttagt ctatagagta aaagaagaaa agaaagtaaa
     2641 agattatgat gtaaagtttg atttacttaa atcatatgat gaatacttta ttgctaggga
     2701 aataaaacat ataatatcag gaatgtttga atgtacttta aatgatgaag aaattattta
     2761 tttaactata ccgttagtta gtggaaatgc accagcatca gaatgtgctt taaatagtag
     2821 tagaattagt aaaaacattg atgagttgat ggaaaatata tttaatcaaa tatatgtcga
     2881 tatgggtatt tttataaatg aggatgaatt aagagtaggt ctaggatatc atcttagttt
     2941 tactttaaac aggctattat ttaatataaa gttaaaaaat gttttgttag aagagattaa
     3001 gcaaaattat atacttccat ttaaattagc acaaatagca ggaaaagtaa tagagaaaaa
     3061 gtataactta gaagtttcag aagatgagat tggatatata gcaatccatt tcagtggata
     3121 tttagaaaga aatagcagta gattctattc aataaaaaaa atagcaatta tatgtagtac
     3181 aggtctagga acagcaaaac tattgaaaat aagagtagaa aaactgatag gaaataatcc
     3241 taaaattgat actctttcaa gttttaattt gagaaatatt aacttagatg aatatgatat
     3301 agtttttaca actattgatt tagacacatc aaatataaat acaatagtat taaaaataaa
     3361 tacaatattt gatgaaaaca aactaagaga acaattaaaa acagtattgt gcctaagaga
     3421 agggaatatt gacactacta attctacaaa tttactaata aataatttgt tagatgaaga
     3481 taaatttatg atattaaatg aaaagactat acttgcttca ttagaaaaaa tgatggacca
     3541 tcttatggat ttaggatgca ttgatgataa gtttagaaaa aatatagtca atagagaaga
     3601 aaaatctccc acagtatttg ataaaggttt attatttcct cattctgtaa atgaaaaatc
     3661 agataagttt ttaatggcag ttggaatttt agaagaacca atagaatatg caaatagaag
     3721 tataaaaata attcttatga caatgtttcc atgtgagaat aagatagact cagacttatt
     3781 agtaaaaatt tatgaagaag ttttaaagat aggccaagat gtaaaattaa cgaataagat
     3841 aagtaaatgt agaacttttt tagaatttaa aaaggtgtta cttaaaaacc ttatataact
     3901 attgtacagc atttatagtt gttatacaga tttaaaaata atgtgatttt tggaaggtgt
     3961 ataaatatga aattattagt gattttattt gttgcttata ttttaaatac tatatttata
     4021 atgaagcaga ataaattgta ttttaaaact ttaaatagag ctaaaaaaaa gggtgatata
     4081 gtgtctacag gcaaaaagaa aagctatttt tctaaaggaa gtatagccat tatatcttca
     4141 gattcagatg ggtttataaa gtttggagaa attttaaaag gacgtacagt aatggctaaa
     4201 tttaaagaga ttgaagaaat agaaggatta gatatatata gtgcagagca aaaatttaaa
     4261 gatgaagaga gtattgtaca agctataaaa tttataaaag aaaaaataaa tgtttctttt
     4321 aattaactca atataaatat aaattatcag gaggaatgtc atgaaatatg aagttaagat
     4381 aagtgggata ggcagtttag ttaaagagtt aatggatgaa agcaattgtc ttattatata
     4441 tgatgaaacc attaatgatg acgacttaaa ggatatatct gtaattcatt caatagcaac
     4501 gttaaaaagt gatgttgaaa taggagatac acttactata gggaatagag attattgtat
     4561 agtttcagtt ggtgatattg ctcagaagac tcttagagaa attgggcatt gtacaatcaa
     4621 atttgatgga aaatgtgaag taaatttacc aggagaaata catgtggaaa taggaaatcc
     4681 agatataact attggagatt taataactat ttcttaattt gaaaataaaa ttattataac
     4741 aggaggaatt ggtatgttag atttaaacag aaaacttgca aaattggaag aggaaggtaa
     4801 aaaaataaaa gtagctctta taggagctgg gcatatggga aatggaatgg taagccaaat
     4861 ggcaaatatg aaaggtattg aagcttctat tgttgtagat ataaatttgg agttggctca
     4921 taaagctttt actgatgcag gaatagatga agaaattata gataatgtaa caaatgaaaa
     4981 ggatgcagaa ataaaattac aagaaggaaa agtattgaca tgtaatgatt ttcttgtggc
     5041 ttgtaaaact aagtcaatag atgtagttat agatgcaact ggaggtattg caattggtgc
     5101 agagatagca ttaaactcta ttttaaataa aaaacatata gttatgttaa atgctgaaac
     5161 tgattgtgtt gttggaccaa tacttaaaaa attagcagat gatgctggag taatatttac
     5221 aggttctgca ggagatgagc caggtgcagt aatggaatta tttgattttg cagatgcaat
     5281 ggggtttgaa gttagagtga taggtaaagg taaaaataat aaattagatt tagattgtaa
     5341 tcctgataca gttagagaag aagcagaaag aaaaggagct tcacctcata tgatagcttc
     5401 ttttaaagaa ggaactaaaa caatggtaga aatggcttta atgtgtaatg caactggatt
     5461 tgtgcctgat gtaagaggtg gtcatggtat tgaagctaca gttaatgaag tacctaagaa
     5521 atatgcatta aaaagcgaag gtggagtatt agataattat ggtgtagttg attttgtaaa
     5581 tggtatagca cctggagttt tcgtagtagt agcccataaa ttaaaagcag ttaatgatga
     5641 actaaaatat ttaagtatgg gtgatggacc taattacata ttatatagac catatcattt
     5701 atgtagttta gagactcctt tatcagcagc tatggcagtc ttagaccata aggctacaat
     5761 agtacctaaa gctggactag tagcagaagt tatgactata gctaagaaag atttgaaaaa
     5821 aggtgagttt atggatggct atggagcata tacatgctat ggtactatag aaaaatacga
     5881 tgtagcaaag gctatgaatg ctgtacctat tggtttgata agtaaaaaga ctaaagttgt
     5941 aaaagatatt aaaaaaggtg aagttataac ttacgatatg gtagagatag aaaaagatac
     6001 tactttatat catttaagac aattgcaaga aaaaatattt gggtaagatt tgatggtaat
     6061 ataaatagat attagtaaaa agggttgatg gttattttta aatcaaccct tttaaatttt
     6121 tatatattct tgttaggata ctttctttaa aagtatatta taggttattt gtagtcgtaa
     6181 aaattatttt gaattagttc tgcaattt
//
LOCUS       DAMKMI010000179         6187 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMKMI010000179 DAMKMI010000000
VERSION     DAMKMI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.185"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            128..1009
                     /locus_tag="PC429_003412"
     CDS             128..1009
                     /locus_tag="PC429_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF3848869.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            1391..2194
                     /locus_tag="PC429_003413"
     CDS             1391..2194
                     /locus_tag="PC429_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848870.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            2206..3336
                     /locus_tag="PC429_003414"
     CDS             2206..3336
                     /locus_tag="PC429_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF3848871.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            3340..4749
                     /locus_tag="PC429_003415"
     CDS             3340..4749
                     /locus_tag="PC429_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848872.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            4960..5469
                     /locus_tag="PC429_003416"
     CDS             4960..5469
                     /locus_tag="PC429_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF3848873.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
BASE COUNT         2519 a          548 c         1099 g         2021 t
ORIGIN      
        1 atgttatact atttataaaa gtaggtataa agattaaaat acataatcaa atatgtataa
       61 gaggttttaa atagtattgt taatgaactt gattattgtt tataattaca aattggagga
      121 gataaacatg aaaattactg aaggatatat gccttttaaa ggttttaaaa cgtattaccg
      181 tatagtagga gaaaatacgg aagggaagaa gccattagta ttacttcatg gaggtccagg
      241 ttctacacac aactattttg aggtattgga taagatagct gaaagtggta gacaagtaat
      301 aatgtatgac caaattggtt gtggaaattc atttgtagaa gggcatcctg agctatttaa
      361 tgcagatact tggatagagg agcttattga actaagaaaa catctaggac ttgatgaaat
      421 tcatctgtta ggtcaatctt ggggaggtat gcaggctatt tggtatgcta ttgaatataa
      481 accaaaaggt attaagtctt acatcctttc atctactctt tcttctgcaa aactttggga
      541 gaaagaacaa aaaagaagaa tatcatatat gagtgaggtt gaccaaaaag cattacttga
      601 tgcagtaaat actggagatt actctagcaa agaatataat gatgcattag aaagatttat
      661 ggaaatgtat tgtgcaggtg aagtgacaga agattctcca gagtgcctga gaagacctaa
      721 aaaatcaggt tctgaagcat atattgtagg atggggacaa aatgaatttt ctcctacagg
      781 aactctttct ggatatgaat ttacagatag attacatgaa ataaaagagc cttgtctagt
      841 aactagtgga gcaatagatt tatgttcacc atatattgca aagactatgt atgatagaat
      901 acctaatagt aagtgggaat tatttgagta ttcaagacat atgccattcg ttgaagaaaa
      961 tgagaaatat ataaaagtct taactgagtg gttgaatgcg aacgattaaa aactatataa
     1021 acttatataa aaaaatgagt gtctcaaaat gaacttttta gttcattagg cactcttttt
     1081 gcatgaaatc aaatatattt tcattatttg aacgataaaa tgaggcttat atctactttg
     1141 ggaaagtaat tttttattac tagcgttaga ttctaatcta attgtatacg tattgaaata
     1201 atagaaatat attggtgttt ataacttaat tttattgttt tttagaaatt tttttatata
     1261 ttggtataat tattctgttt aaatttgatt aaaaagtata ttaattataa taagaaacaa
     1321 ttatataaat aggaatagat tattataatt aatatagata taaaaataat tatagaaaga
     1381 aaggcacatt atggaaaaat tgagagttat tgaaatgata aaaaataatc ccaatattat
     1441 agctacaata gataatccaa ctgatgaaat gaaattgtta gctattaaag aaaatgggct
     1501 tgtactagaa tatataaata atccaaccag agagatgcaa gaactagcaa tagataataa
     1561 tattagagca attaaattta tagataatcc aacagaagat atgatgataa aagcagtaaa
     1621 tgagggatgg agtatactgg attatatcaa aaacccaact gataaagtta ttgaaatggc
     1681 tataaatcaa gcaggatggg caattaaata tgctaaaaat ccaagtgaag aattacaact
     1741 gttagcagta agaaaaaatt atgattcgat aaagtttata aaacaacctt atgaaagtgt
     1801 acaagaggag gcagtgagaa taagttatga tgccttgaga tatatagatt cacctagtta
     1861 taatgcagaa cttatagcta taaaaaataa tgaagcagct attagcttta taactgattt
     1921 agataaaaat aaaatgttgg aatttttaaa agtgaatatt ttagtgatta agtacattct
     1981 gaaaaaagta tctaaagatg aattagagag tgtattaaaa gaagtattat ccaaagaaga
     2041 tgttgaggaa aagtatatta gagatttttt gaattgtagt gttatagata gaaatagcga
     2101 aagtatacca atagataaaa ttatgtttat ttataaatat ggaagtaaga cagcaaaaaa
     2161 aatagctgta gatgagaaat taaagattat gtaggagaga gaaaaatgaa ttttatagac
     2221 acattaaaaa gcgttgactt aatattatcc actggagaag tacctttaat agtaggtgaa
     2281 agtggaatag gaaaaacagc tttagcaaat aagcttgcca aagaaaatga ttggagttta
     2341 attgttattg atggaaactt acttaaagag ggtgaaatag gtggtcttcc aactatagaa
     2401 tcttatgtag gagttaattc taatggatat aagactgaaa agaaaacaac agtgtatgct
     2461 gttcataaca aattaagaga aattgatgaa gagatatcta aaagtaagac agttctttta
     2521 tttatagatg agataaatcg ttgtgagcat acagtacagc aagaacttat gaatttaatt
     2581 ttaaatagag aaattaatgg atataaactg catgatgatg taaagatttt agcagcaatg
     2641 aatccatcaa gtaaatatgg ttcagatttt gattatcaag tggtagatat ggatgcagcc
     2701 caagaaaata ggtttgtttg gttaaatatg gagagtgacc atacacaatg gataaaatgg
     2761 gcaatagatg agggtattga aagaaaagtt atcgagttta tttccacttt tccagaatat
     2821 ctgcataaaa taaatgaaga tgatgtaaga gccactccta gaagttatga gagagtatct
     2881 aaaatttata aagtttataa agaaaaaaat aattctattc ctagagcagt atttttaaat
     2941 gttgtaaagg gtaatgtagg taaagttatt gcagaagaat ttataagttt tattgaatca
     3001 aactctgaac cattaatatc ctatgaagat gtttttttag gagaatctat tgatgagtct
     3061 attgtagaaa gagtaaaaaa tgagagtcat acaagacttt atctatcagc tatgaacatt
     3121 ctaaaagact tagaattaaa tattaagaat gataaatatg aatcaaacca ctatattaat
     3181 agatttattg aatttttaaa aatgtatcct gtagatttaa tgataggtat tatgaaggat
     3241 attagaaaca gttatattga agtatataaa aaagctatag aaaatgaaga gtttgtaaaa
     3301 tcatattttg aatcttatag tttaataagg ggataatgta tggaaaatta ttttgataaa
     3361 caagcaaaag agctttataa taaagcaagt gaaattataa atactcattc tatgctaaag
     3421 gcaaatcgtg ctaatgagaa atttgatata gatataccac aagattttaa gtatgagttt
     3481 tttagtcttg tagataaagt gaatttaagt cttatggaag aagaagataa tttttatgga
     3541 tattttttgt ttcagatgtc aagagaaata agatttgata taagtagtcc tacaggtgtg
     3601 aattttaagg gagctaaata tgttatatat tttaatccaa taatcttttt aacgcttgac
     3661 ataaagcaaa tggagactac aattaaacat gaaattcatc atatattatc tatgcatttg
     3721 atgagagcaa aggagttaaa aggaaagtat agtacattag caattaatat ggcaatggat
     3781 atagtagtaa ataaatttct aaacaattta ccaccatatg caactacctt agagtgggta
     3841 aatttaaagt attccttaaa acttgaacca tatgaacctt ttgaatatta tgtagaaaag
     3901 attcagactg aattagattt gatggaagtg gatgaagatg gtgaggaaga tgatagtgat
     3961 aaatatggag atatagaaat tgattatagc cccgaaagaa ctcatgatat atgggatgaa
     4021 tccaatgaga tagatgaaaa tacccttaga gaatttactg aaaaatttat taacagttct
     4081 caaaaaggta aagttcctgc ttatttagaa agtatgatat catcacttaa aaatagtaag
     4141 ggtgaattgc catggaattt atatcttaat agaataatgg gaacagttga aaataataaa
     4201 aagaagacta taacaagaag aaatagaaga caaccaaatc gtttagattt gaggggagaa
     4261 cttagaagtc ataagtcaga aatagcagtt gcacttgatg taagtggaag tattagtgat
     4321 gaagagttta aacaagctat taaagaagtt cttagtatag tgaaaaacta taatcatgaa
     4381 attacaatta tagaatgtga taatgaaatt agacgtgtat ataaagtgag aactgaaaaa
     4441 gatataaaag atagaattag gataggtgga ggtactagat tttctccagt ctttgagtat
     4501 gctaataata aaaagattaa tttgttagtc tactttactg atggtaaagg tgaaaataaa
     4561 ctacaagtaa tacctagagg gtataaaatt ttatgggtta tttctggaag aggagataaa
     4621 atttcattaa aagaacctta tggagcagtt aaaaaactta ctaaggttaa aacaaatgat
     4681 gaaatattag aaataaatga tgtaagaaat gatgggtact caatgaatag ccaagcacca
     4741 attttatagg tattcacagg agataagctg tattaattta ttttgatatg cttgttataa
     4801 aaatctaaaa aatacattaa tcaatggtta atgtattttt taattttaat aatataagtt
     4861 ttacttacat ttaagtctta aaatgtgtta aaatataata tatagcagat agttttggca
     4921 aggtggattt agttgtcaaa actatttttt ttattaagga tggtgatgtt tttgtttaag
     4981 attgatgatt atataatgta tggtatgaca ggagtctgca aagtcatgga tataacaaat
     5041 gaacgattta caaatggaat aaaaaaagag tattatgtat tgagtcctat ttattctaat
     5101 aatacagtaa taaaaattcc tgtagataat gaaaaagtac caatgagaaa gttactttct
     5161 aaaattaatg tgctttcatt aataaatgat attcctaata tggatacatc atggatagat
     5221 aatgaaaaat tgagaagtga gcaatttaaa aaaatactca gaggtggtaa atgtgaagag
     5281 ttaataaaat tagtaagaag tatagataat aacagagagt atgtaaaatc tattggtaaa
     5341 aaaacacatc aagcagatga taatataatg aaagaagctg aaaggttatt aaatgaagaa
     5401 tttgcaacta ttttagatat ttctcctaat gaagttagtt catatatttc aagtcatata
     5461 cctcaataaa gtgttttagt atcctttatt taatctattt taagtattat ttatgtatgc
     5521 tggttttggt aaaataaaat atataattaa attatataaa aaataataat gattgtattg
     5581 aagaattaca gaatagtgcg gagagcctac tcctttaggt gtgagatgga tatcacttaa
     5641 agatttaaat tgttattgta gatgttttta atataaaaca agattaataa gtgtattgta
     5701 agctttaagt gaaattgata ctataatgag ttacttagat tttaaaatat ataatattat
     5761 aaaaaatcct tttaggtttt gaatgaataa gatggatact atgttgaata gaatccatct
     5821 tatttaagtt ttatttatgt tgtttatttg taagcattat ttttgtacat gaaaacacag
     5881 tgtaaaaata tgcttagaat tatgaagttt tattaaaaag atattcgaca aaataagact
     5941 aaaaatattc ttaaacttat aactaagata aagattgtat atttatagtg tagcctgaaa
     6001 aattgtggat gtacgaaatt tatttagtat tttattgaaa caaagtagaa gaagtactat
     6061 agtttatctt tgaatagatt gttatttttt agtgaaaaag ttattaggtt aatacataaa
     6121 tataatagct tataaatagt ttaagcatgt taaaaaagta gttattagag atatatagtt
     6181 acattgc
//
LOCUS       DAMKMI010000180         6107 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMKMI010000180 DAMKMI010000000
VERSION     DAMKMI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.186"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            27..911
                     /locus_tag="PC429_003417"
     CDS             27..911
                     /locus_tag="PC429_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF3848874.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
     gene            1010..2185
                     /locus_tag="PC429_003418"
     CDS             1010..2185
                     /locus_tag="PC429_003418"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF3848875.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            2313..3167
                     /locus_tag="PC429_003419"
     CDS             2313..3167
                     /locus_tag="PC429_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848876.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            3211..4446
                     /locus_tag="PC429_003420"
     CDS             3211..4446
                     /locus_tag="PC429_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF3848877.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSQSKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLI"
     gene            4547..5488
                     /locus_tag="PC429_003421"
     CDS             4547..5488
                     /locus_tag="PC429_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF3848878.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(5707..6060)
                     /locus_tag="PC429_003422"
     CDS             complement(5707..6060)
                     /locus_tag="PC429_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF3848879.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
BASE COUNT         2348 a          611 c         1124 g         2024 t
ORIGIN      
        1 tcaacttatt gaatggagga ataaaaatga aatttgtgac tttttgtagt agcaatgaag
       61 aaaaaatagg tgtatttaat tcagaaacaa atagtatata tgaaattaat agtttaggat
      121 taagtaaatt atatactgat atgaatgatt ttattgaaaa cgtatctaca ggagatttag
      181 aaaaaataaa aaacaattca tttgaaaacg caaagtgtta taagttagaa gaagttaaat
      241 tatgttcccc aattgtaaga cctaaaaaag atattatatg tttaggtcta aactataaag
      301 accatgtcaa tgaaatccca gatggagtta taaaaaacgt agttatgcca gattatccaa
      361 tttatttttc taagagagca gacaaaataa ttggagtgga tgataagata agcttacatg
      421 gagatttggt agagaagttg gactatgaat cagaacttgc agtaatcata ggtaaagaag
      481 gtataaatat atcaaaagaa gatgcttatg aatatatatt tggatataca atagtaaatg
      541 atataagtga aagagcactt caagataaac atgttcaatg gtttagagga aaaagcttag
      601 acacacatac atctatggga ccttgtatag tacataaaga agaatttgaa catcctttaa
      661 aattagatat aagtagtgta gtaaatggag aagtaagaca agattctaat acagagtact
      721 ttatatttga tatacctact ataataaatg atttatcaag aggaatgact ctaaagccag
      781 gagatataat ttcaactgga actccagcag gggttgctat ggggatgaat cctcaagtat
      841 atttaaagca tggcgatata gttgaatgta aagtcgaagg aataggagtt ttaaaaaata
      901 tagttgatta atggataaat atgccatata attttattag tacgaataat ttaattaata
      961 taggattaat caaacttatg attaatttat tatagagagg gatgagtata tggcaagaga
     1021 actagaatta aagtatggct gtaatccaaa tcaaaaacca tcaaagattt atatgaagaa
     1081 tggagaactt cctatagagg tattaaatgg aaaacctgga tacataaatt ttttagatgc
     1141 atttaatagt tggcaacttg tgaaggaatt aaaagaagct acaggtcttc cagcagcaac
     1201 ttcatttaaa catgtaagtc cagcaggagc agcagtaggg gtacctttat cagatacttt
     1261 aaagcaaata tattttgttg atgatttaga gctttctcca ttagcttgtg cttatgcaat
     1321 ggcaagagga gcagatagaa tgtcttctta tggcgatttt atagcacttt cagatgtatg
     1381 tgataaggag actgcaacta ttatagcaag agaggtttct gatggtataa tagctccagg
     1441 atatacagat gaagcccttg aaatattaaa aggaaagcgt aaaggcaact ataatattgt
     1501 aaaaatagat gaaaattata ctcctgaacc tattgaaaca aaggatgttt atggaattac
     1561 ttttgaacaa ggtagaaatg aaatattaat aaatgaagac ctattaaaag atattccaac
     1621 tgataataaa atttttacag atagtgcaaa acgtgattta attatagctc ttattacatt
     1681 aaaatataca caatcaaatt ctgtttgtta tgctaaagat ggacaagtaa taggggtagg
     1741 tgcaggtcaa caatcacgta ttcattgtac tagacttgca ggtaacaaag ctgatacttg
     1801 gtatcttcgt caacatccta aagtattaaa cttaaaattt aaaaaggata taggtcgtcc
     1861 agatagagat aacactattg atgtatatct ttctgatgat tatatggatg tgcttgctga
     1921 tggcatatgg caaaactttt ttgaagaaaa accagagcct ctaactggag aaggaaaaag
     1981 agcatggctt aaaactttaa caggagtagc acttggttca gatgcatttt tcccatttgg
     2041 agataatata gaacgtgcaa aaagaagtgg ggtaagtttt atagcacaac caggtggttc
     2101 tattcgtgat gataatgtta tattaacttg taataagtat aacatagtca tggcatttac
     2161 taagaataga ttattccatc attaaaatac tgaaagtata aagataatat agattaagta
     2221 aaaatctaca acaagttttg ttgtagattt ttttatttgc tgtataattt taaatagaac
     2281 tttattttta gatattcaat ttggaggttg atatggcttt aaatataaga aactatagcc
     2341 tattatgtaa tcaaggaaca gctaaattta atgaagatgt agtcggtata aatccatttg
     2401 gggcatgggt tttagatggt gctacaggac ttaatggaaa aaatttagtg tcaactgaaa
     2461 gtgacgcaag gtggtatgtt cagtggtgga ataggtattt atataaaaat ataaataaag
     2521 aagaatcatt aaagagtatt attaatactg gaataataga agtaaaaaaa gagtatgaac
     2581 taagattaaa tgggtttaag gttgaaaaat tagatttacc atctagttct atagttgttg
     2641 taaaatttca tgaaaataaa gtagagtatt ttttattagg agattgtgct ttgtttatta
     2701 aaaatggaga ttccaaagtt ataaaagata ggagtatttg taaatttgat aatactgtat
     2761 ttgatgaaat gagtaaactt tctaacttgg aagagatggc atttgatgag attagaacta
     2821 gtgttatgga tacaatagta agcaatagat tgaagaaaaa tacaaaagag ggctattgga
     2881 tattggattt tgatgaggaa gctgtggaaa attctatata tggatacatt gaaataaaaa
     2941 aagattttca attgatgcta acgagtgatg gttttacaag tgcatgtgat agatataatc
     3001 taataaaaga agaagaattg atacgtatag cagaaaaatt tggtatagga tatatacata
     3061 ataaagtaag ggattttgaa gataatgact ataaagcggt taaagtgcca agatttaaag
     3121 taaaagatga ttcatcttgt ttatatttag atatatataa tgattagact gtataaaact
     3181 atttaatttt agaaaggagg caagtgaaaa gtgaaaattc ttcatataat aacacaaaaa
     3241 ccaaatagta caggtagtgg tatatatttg tctggtatga taaaaggatt cgaaaaaatc
     3301 ggtcataagc aagctgtaat tgcaggtata gatgtaaatg atgatgtaaa ttgttttcca
     3361 agtgaagtaa gtttttatcc agtaaagtat aattgtgggg agttaaattt tcctgttgta
     3421 ggtatgagtg attctatgcc atatgaaagt acaagatata aagatttaaa tatagacatg
     3481 ataaatagat tgaaatatca atttaaagta aatatagata aggctatgaa tgattttaaa
     3541 ccagaactta taatatgtca tcatttgtac cttttaactg catttgttag ggaaatggta
     3601 aaagatataa aggttatgtc tatttgtcat ggaacttgtt taagacagtt aaataccatt
     3661 gatttagaga aagaatacat aatagcaaat ataaggaaat tagatttaat atttgctctt
     3721 catgaaaatc agaaatatga tattataaag acttttggag taagtcaaag taaagtagtt
     3781 gtaattggta gtggttataa tgatgatata ttttataata aaaattataa aataaaggat
     3841 gataaaataa aaatagtatt tgcaggtaag atatgtaaat caaagggact gattccattt
     3901 ataaaagcta tatctaaatt aaaatatagt aaagatttaa tagaagtcaa ttttgcaggt
     3961 actggaagcg atatcgaatc atataatgag atagttaaac tggcaagtaa gtctccattt
     4021 aaaatgaatt tcctaggaaa gttagaacag agagatttag ctgagctatt caacaggtca
     4081 caaatatttg tgttaccatc attttatgag ggattaccag ttgtagtttt agaagcttta
     4141 tcttgtggca cagatgtaat cactactgat attcttggtg ttaaagagtg gattggtagt
     4201 gagattaata attctggaaa gatagaatat gtttctctac cttttatgga gaaagaaggt
     4261 attccaaaag atgaagagtt atatgatttt gaaaataatt tgtataatgc aatagattct
     4321 aagatacaat ctttattgaa taactcaaat aagaaaacat ctgtagatat gtcaaaaaaa
     4381 acgtgggatg gcttagcata tagaataaat gaagttattt taatggatga attgtgttta
     4441 atctaaaatt atttaaatta taaaaaagtg taactatttt tacactattt ttaaatgatt
     4501 ttatattata ctaatactaa atatatttgt tttagggagg taatttatga ggattagtaa
     4561 aaaggtattg gcgttaggta tttcagcatt actgttaagt gttagttttc caacatcaat
     4621 aaacgcacta gataagattg aaaatattca aggagtagat aaatatgaaa cagctgggct
     4681 tatttcagat aagcaagatt ttacaacagc agttcttatc aatgcagatt ctacaatggt
     4741 agatgggatt gctgcaagtg gtcttgcagg agtaaataat gcagctattt tattaactaa
     4801 taaggatgat ataccagaag caacgcttca aagactgaat agagttacta agatatatgt
     4861 aataggtgga gaaaattcta taagtaaaga tgtagaaaaa atgttactta tgagaagaat
     4921 gcaagttata agaattgatg gtgtagatag agtagataca agttataaaa ttgcaggtga
     4981 aatagaaaaa attaaaaaca gtgataagat gtttttagta aatggattta aggatgaagc
     5041 tgatgcagta agtgttgcat ctgttgctta tagagatgga gctcctataa tattgacaaa
     5101 agatatacct tcagcagaag aagacacaga cttagaccca tggtttggag tttctcctgt
     5161 tccagtatat gctattggag gagagtctac attaagtgat tacatagtga gcagatatag
     5221 agcaactaga attggtggtg ttgatagata tcaaactaat aaaaatgtta ttgaaaagtt
     5281 ctataatgga gcaaaagagt tctatataac aagtggagac gatttagttt atgctttagt
     5341 tgcatcacca cttgcaaaaa atgctcctgt tgttttagtt tcaaataaaa gtgataagag
     5401 tatattaagt ggagctagta aagtaacagc aataggtata agtgataaga gtattataga
     5461 gcaatgtttg gatgctgtaa agaagtaatg gagtgttatt agtaaaatga atatacacat
     5521 tgaaaattag tagattgttc attttataat ggtataaaat atatgggaag aggctatcaa
     5581 atggagataa gcctcttttt cattgttaaa attatgaaaa gatactttat tttatataaa
     5641 aaagtatctt atgaactaaa tgtctatttt gatatactat ctccttttct tatattatat
     5701 gattttttat atggaataaa tttcataagt attatcttcc tttaattttt ctaaccaaac
     5761 catctctaaa atatctagtt cttctaaata atctttctct gttttgttat catcatgttt
     5821 tataagttct aaaacttcaa atgtaaatgc tttttcacca tattttttcc attcattatt
     5881 taattctttt atcatacatg agccagcacc aagctgaaat ttgagagagt taaatttgct
     5941 ttttatgtca ttactttttc caaggtaaat ctttttagta atatcatttt ttattatgaa
     6001 tacacctgtg tcaaatttca tttctttgta taattgtttt aattcacgtt ttctatccat
     6061 gattttagat acctctcttt ataattgata tgtaatatgt aagtgta
//
LOCUS       DAMKMI010000181         6031 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMKMI010000181 DAMKMI010000000
VERSION     DAMKMI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.187"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            163..975
                     /gene="modA"
                     /locus_tag="PC429_003423"
     CDS             163..975
                     /gene="modA"
                     /locus_tag="PC429_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3848880.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            985..1776
                     /locus_tag="PC429_003424"
     CDS             985..1776
                     /locus_tag="PC429_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848881.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            1778..2473
                     /locus_tag="PC429_003425"
     CDS             1778..2473
                     /locus_tag="PC429_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3848882.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2460..3191
                     /locus_tag="PC429_003426"
     CDS             2460..3191
                     /locus_tag="PC429_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848883.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3505..4305
                     /locus_tag="PC429_003427"
     CDS             3505..4305
                     /locus_tag="PC429_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF3848884.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4844..5560
                     /locus_tag="PC429_003428"
     CDS             4844..5560
                     /locus_tag="PC429_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3848885.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2354 a          731 c          846 g         2100 t
ORIGIN      
        1 aataactacc tatggaaatg acattatgaa tagatttttt cttaactata cataacaaaa
       61 cataacaaaa cattataaaa cataacaaaa acttagacat cgttttctta gtatgatata
      121 ctaaaaaaga atttacacta atttaataag ggggaatatc ttatgtttaa aaaactttca
      181 gtactagctt tagctatgat tatttcatta agtattactg catgtaattc taaagaaaac
      241 aaagctaatg atgaatctaa tactaaagtg acaacagctg atgattcaaa agaaaaagag
      301 actctttttg tctattcagg ggcaggttta aagaaaccaa tggatgaaat agcaaaaaaa
      361 tttgagaaag aaaataatgt caaagttgaa tatagctatg caggttctgc acagctaata
      421 gctcaaattg agacaagtca caaaggagac gtatttattg ttggttctga accaatttat
      481 tcaaaggcct ttgaaaaaga acttgtaggt gaacataaaa cggttgcaca tcatacacct
      541 gcaatagttg tacctaaagg aaatccagct aatattcaaa aattggaaga cttaaataaa
      601 gatggagtta aacttatttt gggagacaaa gagtctaatg ctataggtaa gactacacaa
      661 aaaatacttg aaaaaaataa tttaaaatct ataaatgata atgttgtctc tacagctgca
      721 actgtaaatg aaatgattgt acagttaaca tcaagtaaag ctgatgctac aatagccaca
      781 aaagattcgg tctttggtaa tgaagatgta gaagttatag aaattgccca agataaaaat
      841 atagaccaac tcatatcagc aggaatcgta aactatagtg ataaaaaaga tttagctaat
      901 aaatttataa attatataga gtcagacgaa gctaaacaga tatttaaaaa atatggtttt
      961 gaacctgtta agtagtgatt aaacttgaaa aataaatttt ttactactct acttactgga
     1021 ttttttgcta tagtcttttt gtttattgta tcaccacttc ttatgttaat cattaaaggg
     1081 ataagttatg ttcctatatg cttaaaatca gtggaagtac agtatgcaat aacacttagt
     1141 attaaaacat ctttaatatc tactatagta tgccttttat tagctattcc agtagcatat
     1201 tttttacata ttactaaatt accatttaaa aaactgatta tacagataat aaatctccct
     1261 atgtctcttc cacacttagt atcaggaatt gctcttttac ttttgtttgg aagaatgggg
     1321 attggagatt ccatttataa gatttttaag cttgatttta tatttacaaa gcaaggtata
     1381 gttttagcac aaatatttgt aaatcttcca cttactatta aaatacttca cacttcatta
     1441 aatgaaagta atgaaaagat gatatttgtt gcacgtaccc ttggatgtaa tagttgggaa
     1501 gcatttagat ttataattct tccaaatcta aaaactggaa tcatatctgc aactgtaatg
     1561 acttggtcaa gagcacttgg tgaatttggt gcagtagcta tgatagcagg ttctacacgt
     1621 atgaaaactg aaattattcc tacatctata tatttaaata tgtctactgg tgatatagac
     1681 attgctatcg gaatagctgt aattttgata tttctctcac ttacatgctt gatgcttttt
     1741 gagatatttt ttaatagaga ggttgataag aattaatatg ttaaaaataa aaaatttatc
     1801 tcttgattta cctaatttta aattacaaga aaatagttta aatattaata atcatgaata
     1861 ctttgtttta ttaggagcta gtgggtcagg taaaactcta tttctagaaa cattagcagg
     1921 aagatatcca tctgctaaag gaaatgtatc atacaaagaa aatttaatat caaacttacc
     1981 tccagaaaat agaaatatag gatttgtata tcaaaatttt gaattatttc caaatatgaa
     2041 tgttgaagat aatataaggt ttccacttaa attgagaaaa atacctatag atgaacagat
     2101 tcttaaggct aaccacttgt ctaaattaat gcagatagaa gatataaaaa ataggtatcc
     2161 taaaaattta agtggaggcg aaaagcaacg agttgctttt gcacgtgcct taatagctga
     2221 acctgatata ttacttttag atgaacctat gagttcattg gattatatta caaaaaaaca
     2281 tataagcaaa attttgaagg atgtctacat aaaatacaaa ccaacagtga ttcatgttac
     2341 tcatgatatt tctgaagcaa tgttttttgc tcataagata ggaataatga acaatggaaa
     2401 aataaatcat atttttgatt taaatgaaga aataaaaaat aaaggagaat cttttttcta
     2461 tgaatatatt tgatattttg agagattatc tattagtaca agtcgataaa tataatttaa
     2521 atatggatat gataagtatt gtgtccaaat ctctatcttc aaaagaagca attggaaata
     2581 ctaagcgtaa agatttccca attatagttg gtaaagaaat aatgcttgaa gctgacttta
     2641 aaggagctaa aggacaagca tttacttcta ctccatctac atttgaaggt tctttaaaag
     2701 acatattatc attagattta catgacaatt ctcatgatag aagtcttttt atagcatcat
     2761 taaatgcagt tatgaaatac ttaggcaaga cggatagaac cattcattgt aaaaataacg
     2821 agccagaggt atgtgcaaaa aaattccctg agtttataaa aatggaattt ggtaatccaa
     2881 aagtagcaat tataggatat caaccagcca taattgataa tattaaggac tttttcgaaa
     2941 caagagtatt agatttaaat cctgaatttg tggatactat tcaatataat gttaagattg
     3001 aagatggtat acgagattat gaagatgtaa tatcttgggc tgatttagtg atttgcacag
     3061 gaagtacatt atgtaataac tcaattataa attttctatc acttaacaaa ccagtttact
     3121 attatggaac tacgatagct ggtgcatcaa atattttagg tttaaaaaga ctctgttttt
     3181 gtagtaaata agcaatcata tcatcaagga gtacacaaat atatatgtgt actctttaaa
     3241 tttaaaaagt tattactttt attctaagta ctaaagtaaa attctattaa aatgaatccc
     3301 gaataaatgt acaatccctt taaatctcaa ttcctatcaa tcattaatat tttgattagt
     3361 cttatacata aatgttttct ttattttaaa ctaataaaaa aattttattc cattaatcta
     3421 ctctatattt ttcagcattt aagaaataat ttttagtact ttgtttgata atataactaa
     3481 gacaaccttt caggagatga ttaaatgaca tttaagaata aaaaatttac tataaataaa
     3541 tataaaattc tatctattat tctagtttta tgtttgattt taaccgcttt tagtagctat
     3601 gtgaattata gtcataaaaa atttaaagat tatgttgtct tctatgttca gcatcaagat
     3661 gatgaagtgt tgtgggctgg gagtgctatt gttaatgcta taaaagaaag aggaaaaaat
     3721 catgtatttg tggttttagt atctactgga tgtggtatat ccgtctttga taaatataaa
     3781 aaatatgaaa acctaacaaa tatacaaaaa tcagaatata gaaatagaga atttttagct
     3841 tctcttaata gccttggtgt aataagggaa aatataatat tattaccaga aacaaataca
     3901 aatggtaata ctgacttcaa ttacatggaa aaaattgctc ttacatttga agaaaaattt
     3961 aaaaatgtaa ctcatatagc acatacttat aaattagatg accatttaca acatttaaaa
     4021 aatggttctg ttatccaaaa tttatataat gctggtaaaa taaaagatgc taaatatttt
     4081 gtaaaaccta aatttgctga taaaataact tttaataata agatagttta taaagctcat
     4141 tctactgagg actatgaaaa ggttaaaaat gcctgtcaac aatacaaaat tgttgatgaa
     4201 ttagaatata gagaaggtat tggctataag tctgaccata aatcatttga taagttatta
     4261 aataataaag atgttcctgc tatacttcat acacctaatt tataagaaat atttattctt
     4321 taatttaagt tataaaaagg aaattatttg agattttgtg atatagcact ttttgtgcta
     4381 tactactttt tctcatacaa ctattaccca tatctaattt catcatctct taaaatatat
     4441 aaagatatca tcattaaaac ctataagcct tattaaatgc tttatacata cctacattaa
     4501 actacctgtt tatattaaac tacattaaaa taaatatttg ttataaattc ttaataatta
     4561 tgaaactcat ttgttattta tatttgttaa tatatgttta aacctaaaaa attaataaac
     4621 caaaataaca taagaagtgt gatagattac cctagatatt ttcataaact tttaccttca
     4681 gattaatatg ggcgtctata cttttaaatt tactatatgc attgacaata tattaaaagt
     4741 actagttttt atttcataat gaacgggctt actgttttct aatacctctt tttggtataa
     4801 aataaaaatt ggttaaaaat aaaatttcaa gggggttaat aaattgaatc aaaatatatt
     4861 ggttgtagat gatgatagag agatagttga tgctatagag ttctatttga gacctgataa
     4921 ctttaatgtt ttaaaggcat atgatggact tgaagcattg gacatactaa tcgaaaatga
     4981 tattaaatta ataataatgg atgtaatgat gcccaattta gatgggctta aaactacact
     5041 taaaataaga gaaaaaagga atataccaat aattctctta tctgcaaaat cagaagatat
     5101 ggacaaaatt ataggtctta atatgggtgc tgatgactat ataactaaac catttaatcc
     5161 actagaactt actgctcgag taaaatctca acttagaagg tatataaatt taggtgattt
     5221 tagtaataca aataactcaa atgatactaa ttatattcta aaaagtggtg gcctagagct
     5281 aaatacagat acaaaaatag tttctttaga tggtgaggaa attaaaatga caccacttga
     5341 atataaaata cttacacttc ttatgtcacg aaaaggtaaa attttctctt ctcatgagat
     5401 ttatgaaaat gtctggaatg aggatgctta tagttgtgag agaactattg ctgttcatat
     5461 tagaagaatt agagaaaaga ttgaaattaa tccaaaagaa ccaaaatatt taaaggtggt
     5521 gtggggcatt ggttacaaaa ttgaaaaact taattattaa tgattctaaa gcaaaattta
     5581 ttttattatt attactgttt ctattagttt ctatattatt tgaagcagtt tatgatttga
     5641 ataataactt ttctaatatt aattattatt atagagagtt tgctctccct tatttttatt
     5701 ttttcggtac tcctgtacat ttgtcaaaag tagaattaga gtttgtctgc ataggtatta
     5761 tattatcaag ttttttattg actatactat gttttacatt gtataaaaaa aataaacaaa
     5821 ctaaagtcaa attcttagag aatgtcataa accttttttc taaggtaaaa atagaactta
     5881 cacttatatt aactttgata ctattaacct taaggtttca tgcatttaca agtaataaaa
     5941 tgttttacaa cactaattta ataatctaca attttataat tatatctata gtctatataa
     6001 tatatgaaaa tattaaatat aataactata a
//
LOCUS       DAMKMI010000182         5967 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMKMI010000182 DAMKMI010000000
VERSION     DAMKMI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.188"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            77..643
                     /locus_tag="PC429_003429"
     CDS             77..643
                     /locus_tag="PC429_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3848886.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            683..1264
                     /locus_tag="PC429_003430"
     CDS             683..1264
                     /locus_tag="PC429_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF3848887.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(1321..2238)
                     /locus_tag="PC429_003431"
     CDS             complement(1321..2238)
                     /locus_tag="PC429_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF3848888.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            2368..3999
                     /locus_tag="PC429_003432"
     CDS             2368..3999
                     /locus_tag="PC429_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3848889.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFVRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            4002..4949
                     /locus_tag="PC429_003433"
     CDS             4002..4949
                     /locus_tag="PC429_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF3848890.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(5234..5497)
                     /locus_tag="PC429_003434"
     CDS             complement(5234..5497)
                     /locus_tag="PC429_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF3848891.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(5596..5841)
                     /locus_tag="PC429_003435"
     CDS             complement(5596..5841)
                     /locus_tag="PC429_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848892.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         2144 a          813 c          979 g         2031 t
ORIGIN      
        1 gtatataata tagctaatat ggttcttata cttgatacca tcaataaaac taaaaatata
       61 aaaaggagaa cttaaaatga gtgttagttt acaaaaagga caaagagtta gcttagtaaa
      121 agataataat cctgtaaaaa atctagtagt aggcttagga tgggatatga ataaattggg
      181 taaaaaaaat tatgacctag atgcatttgc aatagcattg actaaccaag ataaaatgaa
      241 tgttagaggt gacttggttt attttggaaa tcttaaacac ccttctaaag ctattataca
      301 cactggagat aacttaactg gaaaaggtga aggtgatgat gaacaaataa ttgttaattt
      361 agaagacata cctgaatatg tacataaaat agtttttgga gtttctatat acaaagctaa
      421 aaaaagagac caggattttg gtcaaatgaa caactctttt ataagattaa ttgattcaaa
      481 ttccaagcaa gaattattta aatataatct tcaagaagac ttttctggta aagtaacagt
      541 tttggcagga gaaatttatc gtagaaatga agaatggaaa tttaatgctt taggaattgg
      601 tcaaaatgaa gagcttcgtg aattaattaa tacatataag taataattac aataaaaaat
      661 ttataatgag gagttggttt aaatgggtat aactttagca aaaggacaaa aagtaagttt
      721 aacaaaaagt aaccctggtc taaaaaaagt aatagtagga ctaggatggg atattaataa
      781 atatgatggt ggctttgatt tcgatttaga tgcctctgca tttttaactg gaactgatgg
      841 aaaagtaacg aatgatggag actttatatt ctataataat ttaaaacacg cttctggagc
      901 tgttgaatat atgggtgata atagaacagg tgttggtgat ggtgatgatg aacaaataaa
      961 tgtagactta tctaaaatac ctcaaaatat tgctaaaata tcatttagtg ttactataaa
     1021 cgaagcaatt acaagaagac aaaatttcgg acaagttgaa aattcatata taagaatata
     1081 taatgaagaa actaatgagg aacttattaa atatgaattg ggtgaagatt ttagcataga
     1141 aacagcaata gttgtggcag aactatatag acataatgga gaatggaaat ttaatgcttt
     1201 aggttctgga tttgaggatg gacttgcagg attgtgtaaa aattttggcg ttaatatagg
     1261 ctaatatatc taaataaaaa taagagtata ttgatatact cttattttta tttagatata
     1321 ttataaactg tttacacttc tactcttctt tctcgcttgt tttttcccat attctactga
     1381 aatatcaaaa aatgctgaaa tattttctgt aaaaaacttg cttctatgat atgcaatatc
     1441 aaatgtccta tgtaaattaa taccttcaat atcacaagcc cacaaaagat gcttttttaa
     1501 ataatcctca ataagtaatt cggatataac tgcaatgcca aaattatcta caactgctct
     1561 taatatagct tcagagttat aacatgacca tctaatattc attgggattt ttaattcttt
     1621 taattgcttt tctaattgtg ctcttgtacc acttcctaat tcacgcataa ttaatggttg
     1681 atttgaaagt tctgaaactt ttatagagtc tcttttataa aactcatgct tgtgtgaaca
     1741 tataagtaca agattatcat taataatgct ttttgtaaca atatctgggt gcttaatttt
     1801 tccttctaca atcgcaatat ctaattcact attaagtaat ttttttgcaa tcagtcttgt
     1861 atcttctaca aatacttcag gttctataag aggatttacc ttgtataatt ctaacattat
     1921 tggacttatt acacatgtac caacagttac tgttgcccca actcttatac ggatattttt
     1981 tgcacaatgc ttcatacaat cattcatttc ttcatttaca gctaaaagat gcctcccata
     2041 tccaagaagt tttttccctg cctcagttaa atataatttt tttgaaattc tatcaaatag
     2101 cttaacacca tactcttttt ctatttctga aatagcttgg ctaactgatg actgggtaat
     2161 atacatattt cttgctactt cactcatttt accacattca gcaaccttaa caaagatttc
     2221 aagactacga attgtcatat tatcacaacc ttagcaaaca ttagtaaatg cttatgattt
     2281 tattaatatt ttagtatttt acaaatatat tgtcaagaag tataatttta ttatagagtt
     2341 ataaagtaga ttggagtgtg atatattgtg aaagtattaa ttataggtgg agttgctgct
     2401 ggaacaaaaa ctgctgcaaa aataaaacgt gaaatgggtg ataattgctc tgttacaata
     2461 ctaaataaag gtgatgatat ttcttatgca ggttgtggtc ttccatatta cgttggtaag
     2521 ataattgaag ataaatcatc tttaatcgta aatacacctg aaagtttttc taaactaaca
     2581 ggagcagaag ttcgttgtgg agttgaagtt acatctattg atagaaatgc aaaacaagta
     2641 actttgaaaa cgctttcaac aggagaaaaa gaaaatctaa gctatgacaa acttgtaatt
     2701 gctacagggg cagacccaat aaaaccacca atacctggaa tagatttacc tggagtattc
     2761 tttgtgcgta ctccaaatga tgctataaaa ctaagagatt ctattgaaaa cggtattaag
     2821 agagctgttg ttattggtgg aggtttcatt ggtcttgaaa ttgctgaaaa tctttctgca
     2881 atgggtatac gtgtaagtgt tgttgatatg gctgaacatg ttatgcctgg atttgatact
     2941 gattttgctg aatacgttga aaatcatatg gctgataaag gcataatgat ttttacaggt
     3001 gaccaggttg taggtatcga aggtgaaaat aaagttgaaa aacttcgtac taaaaataga
     3061 gctattaaaa cagatttagt ggttatgtca gttggaatcc gtcctaacac aggttttcta
     3121 aaagacacag gtttagaatt tgcccctaat aatacaatta ttgcagatga atatatgctt
     3181 acaaatgaca aagatatata cgtagttgga gactgtgctt ttgtaaaaaa ttctttaaca
     3241 aataaaccta catggtcacc tatggggtca tctgcaaatc atgagggtag aatttgcgca
     3301 caaaatatat ctggaaaatc taaaacttac aatggtgttc ttggaacaac agtagtaaaa
     3361 ttacctgagc tcaatgcagg aaaaacaggt ctaagtaaag aaatagctga aaaagaaggt
     3421 tataatgttt gctctgtaac catagctact gatgataaag ctcattatta tccaggtgca
     3481 tctaatttta ttataagaat ggttgctgaa aaaaatacta gaaaattatt aggtgttcaa
     3541 gttatgggac ctggagcggt tgataaaata gtagatatcg ctgctactgc aataacttta
     3601 ggcgctgacc tttattcatt agaaagtatg gaccttgcat atgcaccacc tttttcaaca
     3661 gctattcacc cttttgtagt ggcagtaaat gttttacaaa ataaacttaa tggagaactt
     3721 gatagtgttt tacttgacga aattgaggat tttgacagtt ggactaaact tgatgtttca
     3781 aaagctcctt ctattcctag tttacgttat attcctgttg gtgaaattaa tggagaaatt
     3841 gaaggccttg ctaaggatga aaaaattctt cttatatgtg ctaaaggtaa aaatagctat
     3901 atggcgcaaa atcgcttaag acgctttggc tatacaaata cgaaagttct tgaaggtgga
     3961 atactatttt atccaaatct taaggtaatg gaggagtaaa tatgagtaat gttacagcag
     4021 aacaaaaaaa ggaagtaaaa ggtagaggtt ttctaccaag cagggatgga gaacattttg
     4081 cagcacgtat aataactgta aatggtgtaa taaatgcatc acaagcaaaa aaaattgcag
     4141 aagctgctga aaaattcgga aacggtcagt tagcatttac tactagacta acagtagaat
     4201 tacctggtgt aaaatttgaa gatattgaag ctctaagtga atttattgct agcgaaaatc
     4261 taataacagg tggaactgga tcaagggttc gtcctgttgt ggcttgtaaa ggtactgttt
     4321 gtgttcatgg tctagctgat acacaggcac ttgctgctga aatacacgaa gaattttaca
     4381 aaaattggta tgatgtaaaa ctacctcata agttcaaaat tggtgttggt ggatgcccta
     4441 ataactgtat taaacctgac ctaaacgatc ttggaattgt tggtcaacgt gtacctgact
     4501 atgattcaga gctttgtgtt ggttgtaaaa aatgtgctgt tgtagaggtc tgtcctgtta
     4561 aagcagctaa attaacagat aaaggtaaac ttgaaataga tagtaatcta tgtaataact
     4621 gtggtaagtg tatagaaagt tgtaattttg atagtattga agaaaaagaa tctggttata
     4681 aagtctatat aggagggaaa tggggaaaat cagttcgtcc tggaacacag attgatagat
     4741 tattttcaaa agaggaactt atgactttaa ttgaaaaagc aattctttta tttagagaac
     4801 agggtaacac aggtgaacgt tttggaataa ctatcgatag aatcggtgct gataaattta
     4861 tagaaatgct tttatcagat gaagttttgg aaagaaaaga agagatacta gaggctcttt
     4921 tacaccttac aggtggagct gtatgctaac aataaatatt ttaattgtac agataaccta
     4981 tggaaaaaat tttagttaag aataattaaa attctatata tgatatatga taaatgaggc
     5041 tatctcaaaa cacagatacg cctttttttc attgttgaaa tgataaagta tatttgagtt
     5101 catataaaaa agagtgtctc gtgaattata aaattcattt tgaggcaccc acattatttt
     5161 ataataattg cttattaatt ttattttaac tatataaaaa gtacaaaaag taagaatatc
     5221 caatattcta aaattaattt tgaattgtat tgattgaaaa gtttactatt ccactatttc
     5281 catcttgatt tactactaga taccatgttc cagtattagg aactctcatt ctacatggat
     5341 ttttctttgc aaatccacca tagtaatcat aatttctttg atttttaaat ttcacataat
     5401 ttatatggtc taacaatatt acattggctt gtttctctaa atttacctct acaatatccc
     5461 ctttttttaa gttcatctta ctataactat attccattga attactcctc cagtatcgaa
     5521 taatctttgt tatttactca tctcaaatat gtaaaaattc atttaaaata ttacaaatac
     5581 tatataatta gactattatc cagttttaac cttatttatt acaatccaaa ggttattaaa
     5641 aatattattg gcaacaactt ctccatccca tacaactctt tcttctttat tttctaataa
     5701 agcatagtac ccagagtcag aactttcatc tttatagact gtaacataaa actcttcatc
     5761 actatattca ggtatatttt taaagtcttc agatatttta catgttaata tataaccttc
     5821 taattcttca tctataaaca aagcaattcc tccaatctct atatttttac taattatacc
     5881 atattatgtc ataaactttt tattttatta caaatttcta ttttttcttt actttataaa
     5941 taaattatat aaattttcac tttttaa
//
LOCUS       DAMKMI010000183         5849 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMKMI010000183 DAMKMI010000000
VERSION     DAMKMI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.189"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            61..714
                     /locus_tag="PC429_003436"
     CDS             61..714
                     /locus_tag="PC429_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF3848893.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            855..2126
                     /gene="brnQ"
                     /locus_tag="PC429_003437"
     CDS             855..2126
                     /gene="brnQ"
                     /locus_tag="PC429_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF3848894.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2328..3515
                     /locus_tag="PC429_003438"
     CDS             2328..3515
                     /locus_tag="PC429_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF3848895.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3745..5769)
                     /locus_tag="PC429_003439"
     CDS             complement(3745..5769)
                     /locus_tag="PC429_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3848896.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         2053 a          701 c          924 g         2171 t
ORIGIN      
        1 taagttaata tattaaaaaa aataatatca aaaataatat aatttatagt ggaggggaaa
       61 atgactaaaa ttattaaagt gcaaaaacct aaatttacag gagaattaga aattattgaa
      121 aatcttgaag atatacttga agatatattt aatgatgaaa aaatatttaa taaaattata
      181 gaaaatgaag ttataagtga tttagattct gtaagagtta gctttgaatc ttgtatattt
      241 agaaatgtgt cttttgaaaa ttgtaattta aaaaagattg atttacttga tgtggtattt
      301 gaaaactgca atttatcaaa tacatttttt gatggaggaa gtatatacag agttgaattt
      361 aaaaatagta aattgatagg agttagattt gatgattgta tacttaaaaa cgttttgttc
      421 aaggatgttt taggtaagta ttctaacttt tcattctcta aatttaagat agtttctatc
      481 ttagattcaa attttgaaaa ttctgtattt caagaagtaa aaggtgacaa tctagtattt
      541 gagtatacaa atttaaaaaa agcaaacttt aatggaacta aactttctgg catagatttt
      601 actactaata atattgaggg aattgagatt ggtatagatg atattagagg agcaattttt
      661 gatgtgtcac aagcaattga tttaacgaaa ttgatgggaa ttatcataaa atgaaaaaaa
      721 tattcataaa ataaattggg aaaacgttaa caatgtgtga atttttacca gattgacatt
      781 tttctgaaaa taaagtaaga ttatcttaat attcaaaata ttttcaaagg gggtaattta
      841 gtgtgagtaa gcagatgaga gatattttta tagttggttt tgctttattt tctatgttct
      901 ttggtgcagg aaatcttatg tttcctccat ttttagggat ggaatccggc aaagactggt
      961 taattcctct tattggattt gttgttgcag atgctggtat agcaatgctt gttattatag
     1021 caacagcaag atgtaaggga agcatggatg atgtacttag gagagctggt aaagggttag
     1081 caagagtaat gagtgtagct gctctagcat gtttagcact tcttgtaata ccaagaactt
     1141 gcgcaacaac atatgaaatg ggtattatgc ctatatttgg agaccatggt ccagtagcta
     1201 aagctgtatt ttcaataatc ttctttgggc taactttagc atttactatt aggtcttcta
     1261 aggttataga cataataggt aagtatttaa ctccagcgtt attattagta ttatttgttt
     1321 taatagtaaa gggtataatt tctccagcag gacctatgag tcctgagcat atgatagata
     1381 aaaatctatt tggtgaaggt atatcacaag gttatcaaac aatggacgct ctaggtgcag
     1441 catctatggc aactataatg ttgatgtcta taatagctaa aggatataca tcagataaag
     1501 accaaatagg aatgactatt aaagctggtt ttgtagcctg tgtatttcta gcagtagtat
     1561 atggtggtct tacttattta ggtgcaacag tttcaacttt atatgatact agtatacctc
     1621 aagcatcttt attagttgaa ataactagta tattgttata tggtcaagta ggaaaggtaa
     1681 ttttaggtgt aattgtggca cttgcctgtc tgactacttc tacaggttta acagcatcaa
     1741 tatcaactta ttttgaagga ataagtaagg cgagttataa acaactggtt ataggtatat
     1801 gtgtagctag tatgcttata tcaaatttag gtgttgattc tataatagcc atatcagttc
     1861 caatcctaca aactatatat cctgttttat tagcaatagt tgttatggag ttggctggta
     1921 aacatataaa gaatgataat gcttttaaag gtgcagcata tgttacatta gtgattagtt
     1981 tattatcagc tataaatgga atgacaggag ctgttccatt tatacaaagc ttgccacttg
     2041 caggtttggg ctttaactgg gtaataccag ctatattggg tggaatagtt ggtaacttta
     2101 taaaaagcaa caaacaagta gcataataat ttgtaattaa tttaatttta taatatatta
     2161 atttttagta ctatgggtag gtttaatcta aacctaatct acccatagta ctttttagat
     2221 tggattataa tagcatgtat ataatttttt ataatataat ttttctttga agtatgataa
     2281 aattagtaat agtgataaga atgtacaaat ttaggaggtt ttaattaatg cttagaaaaa
     2341 taacagcatt tatgctatcc atatgtttaa ttattggact tacaggttgt agtttacttg
     2401 taggcaagaa agataaagag aaaattatag atgattttaa agttgcagtg gtaactcaac
     2461 cattatctga aaataaagta caatacaata tggtggaaga gatggcaaaa gaatatgagg
     2521 aagagaataa aattgataaa gacaaagatg gtcaaactaa agttaaacaa actataaagc
     2581 atgttgtatt gccagagaat tttacttcta atatagacag tgctattaat aagatagtaa
     2641 agttggctga cgataaagaa gttcaggcta ttgtagtttc aactgaccaa gcagggcttt
     2701 tacctgcttt acaaaaggta aaggaaaagc gtccagaaat aataactatt tctgccccta
     2761 tgggagatga taaaaaccaa ttaagccaat ttgttgatgt gaatctaggg gttagtgcag
     2821 aggaaagagg aaaagtatta gctgaacgtt caaaagaaat gggagcaaaa gcttttattc
     2881 attatgcctc tacagatgat ttgaaagatg ttaatatagc caaaagatta gaaatgatta
     2941 aagagacttg caaaaatata ggattgccat ttgtacaagt taatactcca aatataaata
     3001 ctgaagaaga caaaaacaaa gttaagcagt ttttaaatga agatattgaa aaacaagtta
     3061 aaaaatatgg aaaagatata aatgtatttg gtgtaaatga gtatatggat gaagttatat
     3121 tgactaaggc tttagaatta aagtatatag ttgcagagca aagcaatcca tcaccaatac
     3181 aaacatatcc tagtgttatg ggtctaaaaa tatctgaaaa agatgctcaa aattatgata
     3241 aaataaacga catgataagt gaaaaggcta aggcttttgg catgtcaaat agacttggtg
     3301 gatatcctat gccaatggat gcttttctac catcagtagc aatttatctt gctactgaaa
     3361 tggtgaaaca agatttaaca caagaagatg tatgtgaccc agattattta gaggcgttta
     3421 cagaattaag atttggaatc ggttcagaat ttactccttt aacagaggta ctttataatt
     3481 atcaatcagt aattttaagc caattaattt attaatatta aggaagtgtc tcaaaatgaa
     3541 ctttttagtt tatgaggcac tctttttcta ttaaatcaaa tatattttta tctttttaac
     3601 aattaaaaag aagcttatcc ctatattgag atagcttcgt ttttaatata aataagtttt
     3661 attattttat aaaaaattta tagaaaaata aaaaattata attatatata aattttgata
     3721 aagtaaagat gaattactat aggtttaaat tatattattt tctattattt ttatgccttt
     3781 ctcagttatt gatgagcctt ttcttccttt actagaaatt aagtatccat tgcgtgtcaa
     3841 atattctagc atatttctta cttcgtattc tgtgacatta tattccataa atttcattaa
     3901 ctttgtcctc cccattggtt tcatgtgcaa tgattttagg atagaaatgc atatgttttt
     3961 gtcatattgg tttaaatcat tgaatatgga catattactt gacatatgat tattatttaa
     4021 ttttgaagat atgttttgag gtaaatcatt tattgtaaga taattggaac ccatgtaagc
     4081 catatattca atacaatttc tcagttctct aatattcccc atccaagtgt atgaatttaa
     4141 agcttctaaa agactcttat cgagttttat gtttttatag ggaagttctt ttaaaaatga
     4201 ttctaaaaga agaggtatat cttctaatct ctttctaaga ggaggtaaat ctaaagtgaa
     4261 catacttatc ctataaaata aatccatcct aaatttattt tctataatta atttctctaa
     4321 atctttattt gtggcagcta atattctcac atcaattgga ataatacttg taccaccaat
     4381 ttttctaatt tctttttctt gtaagactct taaaagttta acctgtaaat catgtggcat
     4441 atctcctatc tcatctaaaa atatagtgcc agtgtgagct aattcgaata atcctttttt
     4501 tccacctttt tttgctccag taaaagaacc atcttcatat ccaaatagtt cactttcaag
     4561 taattcactt ggtaatgaag cacaatttat tgctacaaaa ggtttatttt ttctatttga
     4621 atgattgtgt attgactgag taaaggcttc tttacctgtt cctgtttcac ctgttattaa
     4681 tataggatta tctgtaagag ccattttttt tgccttttta atacaatctt ttataataga
     4741 actatttcca ataatattat taaatttata tttagctaca taacctcttt tgacagaatc
     4801 acttcttatt ttttgttcta atatttctat attgtttaca tctttcaatg taattaagca
     4861 accctctata tttttataaa gataaaatgg ttttttagaa agtatgaatt ttttatctat
     4921 ttcgtctatg tatataatca tattatcgat atttaagttc tttgtaattt tatctaagaa
     4981 actatttggc atatattcca ttaaggaagg tgattttata agtttttcat ctagtcccaa
     5041 catattaagt aaagacttat ttacaaaagt aaccttgtta aaggtgttga aaaatattag
     5101 accatcacct atttcatcaa tggtcatgta aagtatagtt tttaatttag atatattatc
     5161 taatgatgta tttaagaaat catgacttag tgtctctttg gacaatttgt atagtttttc
     5221 tataaatttc tcatttttta gttttaatac tactaataat gacatatatg tatctaatga
     5281 gatttttctc caacctaaat caacaatatt ttttatgtat tttggtataa atctaatctg
     5341 acctggtgtt atagctgtat ctaaatcata tatttcatca caatctggat atgtcggtac
     5401 aaaatctata tgatttatac caattttata tagataagta gccatctcaa atgctgaagt
     5461 tttattggtt gtcataagta acgattttgt acctggaggt agtttcatca ttttttcata
     5521 tccaagtttt gaaagtgctt tcgttccatg aataatttca atatcttcct taaaatgttt
     5581 tattaaaaaa ttactagtta aaggtgatag agaaagcact acatctccct ctaaattttc
     5641 ttttattcca tcaagtatat tttgtgtttc tatgattatt tcgtctccaa aaaagtttgt
     5701 taattcttct ttgtagtggt cactattcaa tttatcatgt gttataagga ttaacttctt
     5761 tttatccatt ttatttctcc taacaaaata gtatttattt ttaaagtatt taaagtataa
     5821 ggcaagttat attatacaac aaatagtag
//
LOCUS       DAMKMI010000184         5835 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMKMI010000184 DAMKMI010000000
VERSION     DAMKMI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.190"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(283..2115)
                     /locus_tag="PC429_003440"
     CDS             complement(283..2115)
                     /locus_tag="PC429_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848897.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVE
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2108..4354)
                     /locus_tag="PC429_003441"
     CDS             complement(2108..4354)
                     /locus_tag="PC429_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848898.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4422..4883)
                     /locus_tag="PC429_003442"
     CDS             complement(4422..4883)
                     /locus_tag="PC429_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3848899.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5188..5754)
                     /locus_tag="PC429_003443"
     CDS             complement(5188..5754)
                     /locus_tag="PC429_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF3848900.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1866 a         1085 c          633 g         2251 t
ORIGIN      
        1 ctatttataa taaaatttaa agaataaaat taaatataca ttttgataat cacctccaac
       61 attttcctta ctacatatta taataaaata aacactttgt aaagacataa aattatgtgg
      121 ctatctcaaa atatagagaa tcctcttttt cattgttaaa atgataaaaa tacactttat
      181 ttcatacaca aaagagtgcc ttatgaatta aaaagttcat tttaaggcac cctcatttat
      241 tttatcttat taattacatg tctatgctaa gatagttttt tgttacattg cttcatcttc
      301 ttcaaattga gaattgtaaa gattggcata gaatccgcct ttctttagaa gttcctcatg
      361 atttccttgt tcaactatat ctccttcatt cattacaaga atcatatcag catcacgtat
      421 agttgataat ctgtgggcta taacaaaact tgttctacct tccataagat tatccatagc
      481 tttttgaatc aatacttctg ttctagtatc gacagaactt gtagcttcat ctaatattaa
      541 aattttagga tcagctaaaa tagcacgagc tatagtcaat aattgttttt gaccttgaga
      601 aacattactt gcttcttcat ttaatttcat cttatatcca tctggtaatg tcttaataaa
      661 tctatgaaca tgtgcagatt tagcagcctc tataacttct tcatctgtag cattcaactt
      721 accatatctt atattttcca taattgaacc actaaataac catgtatctt gaagaaccat
      781 accaaacatt tctctaagct cactacgatt aaaatcttta atattatgac catcaatcag
      841 tattgaaccg ctattaacat cataaaatct cattaacagt ttaactattg tagttttacc
      901 tgctcctgta ggtccaacaa tagcaacttt ttgaccaggt tcaacattta ctgagaaatc
      961 atttattata gtcttgtttg ggttatatcc aaagttgaca ttttcaaaat caattttacc
     1021 ttctaaacca tctattgaaa ctgcattttc aacaatttgg acttcttctt cctcttctaa
     1081 aaactcaaat actctttcag aagctgctgc tgttgattgc aataaattgg caacctgtgc
     1141 aatctgagtc attggctgtg taaagtttct tacatattgt ataaatgact gtatgtcacc
     1201 aacctcaatt gttttcttaa ttgcaagcca acctcctaag attgatacca ttacatatcc
     1261 caagtttcca ataaacatca taagtggttg cattatacct gataaaaatt gagatttcca
     1321 cgctgaatta tacaggctat catttagttt atcaaactct tcaattactt catcctcacg
     1381 attaaatgct tttactattg tttggccact gtaagtttcc tcaacttgac catttacatt
     1441 tcctaaatat tcttgttgat atctaaagta tttttgtgat ttctttatta ttcttgaaat
     1501 tacaaacatt gatattggta atattaaaac tgccactaat gtcataattc cacttataga
     1561 taacatcatt atcaaaacac ctatcatagt tgttactgat gttataagct gtgtcatact
     1621 ttgatttaaa ctttgattta aagtatctat atcattcgta attctcgata atatttcacc
     1681 atgcgtctta gtatcaaaat acttcattgg caatctatcc aattttgctg aaatctcttt
     1741 tcttaaatta taggatactt tttgagaaat tccactcatt ataaatcctt gtataaaaga
     1801 gaacagtgca cttatcaaat aaaggaaaag taaaaaggtt aagatttttc caattgcatt
     1861 aaaatctata cctacgtttc ctccagaaac tttactcact agcccttcaa atatctttgt
     1921 agttgccttt cctaaaattt ttggacctac tatagaaaac gacgcactac caatagcaaa
     1981 tacaattact agaattattg atattttaaa tttagataaa tatgcaatta gttttcgcat
     2041 agtcccttta aagtcatttg ctttttgtcc tgctcccata gattttccca taggacctct
     2101 tcccattcta ctcattagct agctcctcct ttgaaagttg tgataaagct atttgctcat
     2161 aaacttcgca attttttaga agctctttat gtgttcctat tccaactatt tcgccctcat
     2221 ttaaaacaat tatctgttct gcatttaaga tagtactaat tctttgtgca actattagaa
     2281 ctgtactttc agcagttttt gattttaaag ccttacgaag agctgcatca gtttttaagt
     2341 caagtgctga aaaactatca tcaaatatat aaacttctgg attttttgct attgctcttg
     2401 caatagaaag tctttgtttt tgacctccag atacatttgt tccaccttga gaaatttcag
     2461 ttttaaaagc ttccggttta gaatctataa attctgtagc ttgagctatt tcagctgctg
     2521 ttctcatttc ttcatctgtt gcgtcttttc taccatattt taagttggat tctatagtac
     2581 cagaaaacaa tactcccttt tgaggtacat atccaatttt ttcccttaaa tcatgttgag
     2641 aaacattttt tatattaact ccaccaagta atatttctcc ttcagtaaca tcaaagaaac
     2701 gaggaattaa atttataaga gttgacttac cactaccagt acttcctata aaggcagttg
     2761 tttctcctgg ctttgctgta aaagatatat ttgttaatat aggttcttct gcgtctggat
     2821 acatgaacga aacattttta aattcaacaa gacccttttt atcttcctta aactcttgtg
     2881 cctctgattt ttctgggtct tttatgctta tatctgacac taatacttca tctatacgtg
     2941 tagctgaaac ggatgctctt ggaagcataa tagataccat tgatatcatt aagaatgaca
     3001 ttattatttg cattgtatat tgtataaatg ccatcatatc tccaacttgc attgctccag
     3061 aatcaacact gtatgcacct ctccaaacta taagaactgt gataaggttc attataagca
     3121 tcatagcagg catcatacat gtcataactc tatttacaaa aagatttgtt ttagttaaat
     3181 ttttatttgc tttatcaaat ctttcttctt catgtttttc agtactaaaa gctctgataa
     3241 ctggaatacc agtaattatt tcacggctta ctaagtttaa cttatcaaca agcttttgaa
     3301 ccagtttaaa cttaggcata accactgaga ataacactac aattaatgat aagatagcaa
     3361 ctactgctac tgctataatc caggccattg aagtatttgt gtttaaaacc tttatcactc
     3421 cacctatagc cattataggt gcatagaata ctattctaag caacataacc atcaacattt
     3481 gtatctgttg aatatcattt gtactacgag taattaatga agctgtagaa aattcattca
     3541 tttcagtatt tgaaaatgac ataacttttc taaatactcc acttcttaaa tttctaccta
     3601 cacttgcggc tactcttgct gctaaaaatc ctactgttat tgtagctacc atactaatca
     3661 ttgctatacc aagcatttta gcaccagtaa ataatatata attattttga gttttttctg
     3721 tatcaatacc tattttctca tattctgacc ttacatatga aacagagctt tgtgttatca
     3781 tactctctgg catactttca aagcctttgc tcatctcttt agtcaatgta tctaactgtt
     3841 cctttggcat tgctgataac aatttaaata tatcaacatc tccactttga gtagcttgtg
     3901 gtggaagttt actcattaac tgagctttca tagcttttac ctctttacta tcgccctcaa
     3961 gccctgatac tattaaaata gctttaccaa aaataacatt taaatcatct atagtgtctt
     4021 tgtctttagt atttaactca taaagagatt catcttttag acctgggtac ttatctttat
     4081 acttaacata atccttttta ttcagcaaag tataattatc tagtacttta tccttactac
     4141 ttttatccat aaataaagta attttattta attcactctc tctaatcaca gatggaacag
     4201 agttttctac tccaccttgt tgtataccta catttactat attggatgtg tattctggta
     4261 aagaaaggtc acataccgct tgaattacca atagacagac tattattact attgataatg
     4321 ccgatttctt taaatattta attaatttaa acatacattt cttcccttct gtaatattaa
     4381 ttagtctaat aacattaaat tgaaaaacat tattttaaac tttaaaaata tttttcatct
     4441 tcatgcttat gacatcctgc ttttaaatta tctcgtactt gcattagaag tcttctaagt
     4501 actatttttt cttcttcact aaatcctaaa aaaaactctc tttccaattc cttgttaaat
     4561 tcttcaactt tattacatat ttctaaacct ttgtctgtca aaaaaatcct agatactctt
     4621 tggtcctttt catcttgttt tctttccaca agtccagctt tttccatcct ctttatcata
     4681 actgtagttg tagatgcttt tactttcctt ttatctgcta attctttttg actttgccca
     4741 tctttcatct ttaaagaaaa aagtatttgg ggttgaccat gatataagcc taatttatca
     4801 aaataaaaat gagttcttat aaaatatatt cgcattaatt gcaagaaaac atcatttaat
     4861 ttttcttcag aattgctatt catacttgcc ctccttattt tttatattac ttagtcggct
     4921 aagcattatt ttactatact gcatgcattt tttcaatata tttataaaaa attcatataa
     4981 atttcattta ttaaatattt ttatatcata tagttataat tttaataaat aatagtaaga
     5041 tttttaatta gtcagctact tatatttttt attgtaaagc ttaaaataaa ccttttcata
     5101 tattttcaaa taaaaaagtt gtcccaaaat aaattttgaa acaacatctt attcaacagt
     5161 atcttgatta tataacatta tgatactcta cttatcttca tatccattta caacaacatc
     5221 taacttccca gtttcaggtg atataattag accatgtaca ataataccat ctggaattaa
     5281 aggatgccct tttattaaat caatactatc ttttacagat tcttctgcag aatcgaatcc
     5341 atgtaaccat tgttttgtat caattccagc actacgaagt gtaagtatta catcattaga
     5401 tattcctcta tctaaaattt taccaagtaa cttatccgta tcaacattac acataccaca
     5461 tccatgatgc cctacaacca taacctcatc tacatcaaat tcgtaaattg caactaaaac
     5521 acttctcata atacttccaa aaggatgcat tatagtagct cctgcatttt ttattagttt
     5581 cacatctcca tttttcaaat tcatagcctt aggcaaaagc tcagttaatc ttgtatccat
     5641 acaagataag actactattt ttttatttgg atgttttgat gtcacatatt gttcatattc
     5701 tttgttctct acaaatgatt tattatattc taatatctct tcaagttttc tcatatgtaa
     5761 tatcaccctc tttaaaaatt tttttattat tatatcacta ttaaagtaca atatatacac
     5821 atataattta attta
//
LOCUS       DAMKMI010000185         5758 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMKMI010000185 DAMKMI010000000
VERSION     DAMKMI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.191"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            132..632
                     /locus_tag="PC429_003444"
     CDS             132..632
                     /locus_tag="PC429_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF3848901.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            689..1675
                     /locus_tag="PC429_003445"
     CDS             689..1675
                     /locus_tag="PC429_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF3848902.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1725..2447
                     /locus_tag="PC429_003446"
     CDS             1725..2447
                     /locus_tag="PC429_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF3848903.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2476..4410
                     /locus_tag="PC429_003447"
     CDS             2476..4410
                     /locus_tag="PC429_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3848904.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKQEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4403..5671
                     /locus_tag="PC429_003448"
     CDS             4403..5671
                     /locus_tag="PC429_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF3848905.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLILLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         2429 a          430 c          977 g         1922 t
ORIGIN      
        1 aatgaattgg aaaaatagtt cgataaaata atttgttttt ataaaattac tgtttaagtt
       61 tatagatttt gtataaaata aagttatgtt attaattttt ttagaaataa aacttttact
      121 aggagacaga tatgagaaaa aatagtatta atatagcact aactgcagca attacagcaa
      181 tctatgcagt tttaacatta tcattgggat ttataagtta tggaccaata caatttaggg
      241 tagcagagat tatgatgctt ttggcatttt tagataaagg atatatagta ggtcttacat
      301 taggttgttt tttagcaaat gtaataggac catatggtgt acctgatata atttttggaa
      361 cattagccac atttataagt gcaagtatga tttatattac tagaaagtta ggtaaacaga
      421 ataaaagtac tttagttata gcttctttat ggccaactgt tataaattcg attgtggttg
      481 gtctaatgtt aaatgtattc tttgggcttc cattaattct atctatgata caagtaggtt
      541 ttggagaatt tgtagtagtg acaattgtag gagtaccatt tttctgcttt ttaatgaaaa
      601 aatataaaaa tgtcattaat attaaatttt aaatagtgta tagtaattta attaattgag
      661 agtattatta gtgaaaagag gtgtttattt gtcgaaggta aaaagattga ctgatgtttg
      721 gatatatatt aatgataagc aaaagtttac aataaaagag ttgtcagagg aatttaatct
      781 ttcagctaaa acaatacaac gatatttaaa agaattaaat aaaatgggac ttccaattca
      841 agcagaacaa ggtagaaatg gaggttatag ggtgctaaat aatagctata tacctcctgt
      901 tatatttaca gaaaaagaag ttatgattat actatttgca cttaagtcac ttcagattta
      961 taattataag ttaatggaaa tagaaattaa ttctataatg agaaaagtaa gttatgaacg
     1021 taaaagcagt ataaaagtag gtatagaaaa tattaaaaga tacattggct ttgtagctaa
     1081 tgatactaat gatggagatt ttaagagtag taatgtaact gagtttttta gagcttcttc
     1141 agaatctcta attttaaata ttaagtataa gtgtggtaat cagattttgg aaaaaaacgt
     1201 atgccctata ggtatatatt taaataaggg agcatggttt tcaccagcat atgattgtaa
     1261 aagtgataaa ataatattag ttcatataga taatataatt gatataaaaa gaacaggaga
     1321 aagtaaaaaa atacatataa acttggaaga atggttacat gaagtatata acaatttata
     1381 tggaaatagt tcaactacta aaaaatttgg tgaaattatt tatctaaatg tattattaac
     1441 taaagaaggt gtttctaaaa taaaggacag ttcttggaat ttagatatgg agctaaatga
     1501 agatggtagt ggaataatta gtacattcat aaaggttgaa gatatagatt gttatgcttc
     1561 gattttattt tttattggaa ataatgctaa ggttatagag ccaaaatttt tgaataagct
     1621 actttatgat aaggccaatg agttaagata tttttatgaa gaaaatatgg tataatattt
     1681 agaaatgttg aaattaaata attattaagg agatatacgt tataatggaa aattttaata
     1741 agaatgctaa aataattggt attggagctg aaggtattaa tataataaat gaagtagaag
     1801 aaaaaataaa agcaaatatg gatatagaaa aaataaacat aaatcaagag attgaaaaag
     1861 aatatgttag aagtctttta gatggtgtag atatattatt tttgatatat tcatctgaag
     1921 ataaacatat tagagatatt ataaaagctg tttcatatat gtcaaacgaa agaagagttc
     1981 tatctatagg aatggattgt tcagagaaag aaaataaaga agatttagag cttgggagag
     2041 agtttaaaat caataatgat agtatattta aatttgttga tttaatgaat atcatggtag
     2101 agtctatttc tgattcttgc atgataaata tagatattac tgatttaaaa gaagctattg
     2161 taggagataa aggaattaag tattcttttg aagagtttga agatacaaag tcatatagcg
     2221 agatagctga cattttattt gacaggatgg aatatattgg tgaggaattt ataagtaaga
     2281 aaggaattgt atttgttgaa ggaagtccag aattttctat aatggaattg aatgatttaa
     2341 taagtaatat tcagagcaag gtagaagaaa gctatgaagt aatattttct ttatatataa
     2401 aggaaaattt aaatggaaat attagagtag gcttattgta taattagtat attttagata
     2461 ttaaggagag tgtatatgtc tgaaaatata agaggtacag agtattggtt aatggaagaa
     2521 tttttttatc aggaaacaga cacaccacag atttttagag aagaaagcat aattgcactt
     2581 ggatttgaca taaaaataaa tttaaaaaaa gtattcagta tggggaaaaa atcagaaaag
     2641 tatattaatg agaattgtaa tgataaagat tttataaaaa agtttatgtc gattaaaaaa
     2701 ggagattatt ttttatataa agcatatcaa aatatagaag aaaaagttaa atgtaatgtg
     2761 tctattggca tagttgaaga agattttgaa aatggatatg aattaagtca tattttaggg
     2821 catacactac cagtaaaatg gatgcataat tttgatacag tttcaaattg taaagtatat
     2881 acaagtgaat tttataaatt aaataataat gatattagct catatttaga aattattaag
     2941 tcacaagata agaaattatt gagaaaaaga atagaagaaa gtgaaaataa taaatggtat
     3001 ttaaaaaact attattataa aaatgtagat aagtgtgtag atgatgaaga attatttatt
     3061 cagcccatta atctagaaca attagaaagc ctaaaaaata taaagacaga tgattatatt
     3121 ttattgtata atagcgagaa agtaatttgt gaaaatgacc atacaatgaa gagtctattt
     3181 ggcgtatgtc aagttatgga agggttcgat aaagagaaag taagtgcttt ggaaggtgga
     3241 tatttttttc ctgttagatg gatagagtat gaaaaggaga atatagacgg aaatgcatgg
     3301 gttatatttg aaaataatag tatagttgaa aagtatgtaa aaagatttac taataaaatg
     3361 ccattaaata ctatacttta tggaccacca ggaactggta aaacatacaa tacaaaacag
     3421 atggtttaca atctagtaag tgaaaccagt ctaagaaaca aatactatac aggttactta
     3481 gattataaaa acaatgtaga attttgtaca tttcatcaat catatggata tgaggaattt
     3541 atagagggtc ttaagtctga tggtgaaggc aattttaagc aagaagatgg gatattaaag
     3601 gatatatcta tagaagcatg ttataatgca ttgaaatttg aaaaaaagat gagctttgag
     3661 ttggaaaaag atagcttgac tttagaaact ataaaagaca ggaaaaagaa attagtatta
     3721 gaaaatatag aatatagtga gagttttaat tttgaagaat cagaaaacta tgttttaata
     3781 atagatgaaa ttaatagagg gaatgtatct aaaatatttg gtgagctaat aacattattg
     3841 gaagatgata aaagacttac taaatcaaat caaactattg tcaaactacc atattcaaag
     3901 gaaaaatttt ctctacctcc aaatttgtat atagttggaa ctatgaacac atcagataaa
     3961 tcaatagcac ttttagatat agctttaaga agaaggttta attttgaaga aattatgcca
     4021 gattatgatt tattgtctat tgtagatggt atagatatta gaaaaatgct atatacaatc
     4081 aataaaagga tagagttttt atatgataga gatcatgtta ttggacaagc ttatctactt
     4141 gctgtagaaa atattaatga tatagtagta gtatttagaa gtaaaataat accacttctt
     4201 caagagtatt tttattatga ttcagaaaaa gtaggtttga ttttaggagg aattggaaga
     4261 aatgaaaaag ataaatacat tgtttataaa gaaaaactta tagcttctga gttatttaaa
     4321 aataataatg cctcaataat ggagagtaaa gttaactatt gtataaaaca aaacatatct
     4381 agtgaggagc taaaaaatat atatgagtaa aaatatatat gttaaggaaa cctatgaatg
     4441 gataagggta ggtaatggtg aaaatgagct tacagaaatt gaatatgaaa aattgcttaa
     4501 atatctagag aacaataatg atgtattaaa aagtaatata atagatatta aatacaaaaa
     4561 acttagattt ataaactatg taggcatcat atgttttgaa aatgtaatac ttgaaatact
     4621 tcctaaattg tcattaagtg ataatcttgt aaaagataga gagatactat tacagatgct
     4681 ttctatatgt aataaaattc caattacaat gaatgaaaaa ataagattga gtttgaaaaa
     4741 ttataatctc ttaaattttt ttgttatgta tttcattgag agtatgcaaa cacagatgaa
     4801 aaaaggtatt tattttgaat atataaataa aatagagaac ttaaatgtta tgaggggtaa
     4861 aatattatta agtacatatg caaaagaaaa aggcatttct ccaatgaaaa taagatgtga
     4921 atatgatgag tatagtgaaa ataatttctt gaatcaagtt cttaaaaaag catgtatatc
     4981 tattttatgt agaataaatg acaattcaat tcaaggcaag attaaaaaaa ttttaagcta
     5041 ttttcaaaat gtagatttaa tttatataga tagaaaaaaa ctattggatt ataaatttta
     5101 taaaaacaat gatagattta aagattgtta tttactagct agacttatac ttttaaattt
     5161 gtctatggat aatagtcaaa ataatcaaga ggcattttct atattatttg aaataaatac
     5221 tttgtatgaa gaatatatag gaattttaat caaaagtata tgggacaatt catttagaga
     5281 aacatatata caagataaga gtaaatttct tttgaaaaat gaacaaacag gtaagaaaaa
     5341 ctttaattta agaccggata tagttttata tgacttaaaa aatgaatatg aaattataat
     5401 tgacactaaa tggaaagcta ttgaagtaga ttcaaatgtt ttttataggt caagtgatat
     5461 ataccagatg tatgcatata taacagcata tgaaaatgca aaaagatgta tattattata
     5521 tccttgtatt caaaaagata agaattatag ttcctggaaa ttatcagaat catttaaggg
     5581 taagtttata gaagcaaaaa ctgtgaggtt agatgatata aaaaacacaa aaaatgattt
     5641 aaaaaagata atttttaatt ataaatttta aaaataagaa ttttaaaatt tataaaagta
     5701 attttatata ggtataaaaa ttacgagtag tataaaaaat ttaataagaa cttaaatt
//
LOCUS       DAMKMI010000186         5709 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMKMI010000186 DAMKMI010000000
VERSION     DAMKMI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.192"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            83..1195
                     /locus_tag="PC429_003449"
     CDS             83..1195
                     /locus_tag="PC429_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF3848906.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1403..1879)
                     /locus_tag="PC429_003450"
     CDS             complement(1403..1879)
                     /locus_tag="PC429_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848907.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGGHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2078..3703)
                     /locus_tag="PC429_003451"
     CDS             complement(2078..3703)
                     /locus_tag="PC429_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3848908.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
     gene            complement(4041..5060)
                     /locus_tag="PC429_003452"
     CDS             complement(4041..5060)
                     /locus_tag="PC429_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF3848909.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(5057..5626)
                     /gene="mocA"
                     /locus_tag="PC429_003453"
     CDS             complement(5057..5626)
                     /gene="mocA"
                     /locus_tag="PC429_003453"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF3848910.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
BASE COUNT         1940 a          966 c          594 g         2209 t
ORIGIN      
        1 tgtatgcaac tctatttcaa aattattttg tgatatatat atgttataat aaatgtataa
       61 aattttgatt ggtgggttat atatgaatgc atttattaga aaaagaaata aaaactatgt
      121 tgtttacctg gaatttgaag atgaaaagac aggtaaaaga aaacaaaaaa atatgggtac
      181 ttttgataaa aaaagggaag caagtaaaag actaaatgag gtaaaagaaa gcttatataa
      241 tgatggtttt ttagtaccaa gcgaaattac agtgtcagaa tttctattag attttctaaa
      301 taaatatagt gaaaatatat ctttagctac atataataat tatgttagta tttgtaagaa
      361 ttacattaat ccatctattg gaaaatataa aatacaagac ttacatccgg ttcacataca
      421 aaactacata gataaactat cttataaact aaatcctcaa tcaattaaga ttcatataaa
      481 tatattgaaa cttgcaataa aaagagctta tagactcaaa ttaataaaag aaaatgttat
      541 ggatagtata gaggctccaa gatataaaaa atttaaaaat gagatttatg atagagaaca
      601 aatgataaaa ttattagatt tagcaaaaaa tacagaaatg gaattaccta ttaatttggc
      661 tattggatta ggtcttagaa tttcagaagt tttaggtcta acttgggata atattgattt
      721 tgaagaaaat acaataacag taaataagat aacttcccga attaatggtt ctgttattct
      781 caaagagcca aagacagaaa gctctgttag aaaaatatct tctcccaaag aattaatgtc
      841 tttattaaaa gaatataaaa taaaacagaa taaaaattta ttaaaatctt ccattagaaa
      901 taataataat ttgttgtttt tcaataagaa atgtgaacct attgctgaag atgttatgag
      961 taaaaaattt aaaagatttt tagaaaagaa tgaattacct catattagat tccatgattt
     1021 aagacactca catgttactt tacttataaa ttctaaagta cctataaagg ttatatccga
     1081 gagagtagga cactcaaata taagtactac tctaagcgtt tattctcatg tacttaaaga
     1141 aatggataaa gaggcttctg acaaaatttc tgaaagttta tttaatgcta attaatatta
     1201 atttatctta gctttaaatt agcttagctt attgagatag attcattaat attaaatgta
     1261 tctatctcaa attaataact acttaaataa tctgttttat tttaatttat cgcctcagtt
     1321 attatacttt tttacgaaaa attatacttt acaataaaaa agatattccc ttatagaaaa
     1381 tatcttttta aatactatat tattatatac ttgctaacat tggaatatct aacatcttct
     1441 tcattacatc atctactttt tttgctttat attcattgta tccaattaaa tatccttttt
     1501 catatgaact ttttaaaatc tctttttcat ataaattatc agatttggct tctgtgacac
     1561 ttaaattata aagcccttta cacccatctt cataaccttt attataccct ttttctgcaa
     1621 aatagttttt atgatttttt tcgttatgta ctgaagatat tgttactcca gaattttctt
     1681 ttgtactagc ataaggacaa aaacctttat tgtgttgatg tgctgaatgt cctccacaat
     1741 aataacgtgt taactctgat ttatttttat tatgatgacc tcctgatgaa ttatgccctc
     1801 ctgagtgagc atatgaaaaa ttccctacac taatcatagc taaactaatt actacagcta
     1861 ctaacctttt cttaaccata atctattccc cccaaaatgt tatatatttt tattatatca
     1921 tctactatat tatatttaaa ttcttaatat acaaaatatt actctattct gctaataaac
     1981 atgtttaaac aacataaaaa acctcctaat atttgattaa aaatttacac actcatgcat
     2041 agcatgtctc aaatattaaa aggttttata ctaataatta tttatctttt ccatacattt
     2101 tagctaattg gtcttgtatc tctttacttt ctaaatactc atcaaagtcc atttttctat
     2161 ccattattcc atttggagtt atctctataa ttctatttgc tatagtagat ataaattcat
     2221 ggtcatgaga agtaaataaa agcactcctg ggaacttttc aagtccttta tttacagaag
     2281 ttatactttc taggtctaag tggttagtag ggtcatctaa tacaagtaca tttgcatttg
     2341 aaagcattag cttactaagc atacatctaa ccttttctcc tccagatata acttgagctt
     2401 cttttaatgc ttcttcacct gagaataaca ttctacctaa aaatcctctt ataaaacttt
     2461 cacttttttc ttcagagaac tgtctaagcc agtcaaccaa agaatattct actccgtcaa
     2521 aaaacttatt atgatttttt ggaagataat cttgagaagt agttactccc catttatact
     2581 ctccactgtc tggttcaagt tctcccataa ctatgttgaa aagtgcagta gtagctattt
     2641 catctcccat aaatactact ttatcatctc tatccaatct aaatgacaca ttatcaagaa
     2701 ccttaacccc atctatagtt ttagttaaat tgtgtacttc taaaacttca ttcccaattt
     2761 ctcttgcagg tgtaaatcct acatatggat atcttcttct tgatggttgt atatcttcaa
     2821 cttctaattt ctctaactgt ttctttcttg atgttgcttg tttagccttt gaagcattag
     2881 aactaaatct agctataaat tcctttaatt gagctatctt ttcttcagtc tttttatttt
     2941 ggtccttagc caattgtaaa gccaattgac ttgactcata ccagaaatca tagtttccaa
     3001 cgtacatttg tattttacca aaatctacgt caactatatt agtacatatt tgatttagga
     3061 aatgtctatc atgtgatact attataacta tagattcttc taaatccatt ataaaattat
     3121 ttaaccagtt tattgattta aaatctaagt ggtttgtagg ttcgtccatt aatagtattt
     3181 caggcttacc aaaaagtgat tgtgctaaca ataccttaac cttttcccca ccaactagtt
     3241 ccttcatttg tttgtaatgc atatctttcg ttatgccaag gcccataagt attttctcag
     3301 cattagtttc tgcatcccat ccgtcaagct ctgcaaactc accttcaagt tcggcagctt
     3361 ttatcccatc ttcttcatta aagtcttcct tcatatacaa tgcatctttt tctttcatta
     3421 tgttccaaag tctctcatga cctcttataa caacatttaa aacagtttct tcttcatatt
     3481 caaagtggtc ctgttttaaa actgacattc tttctttatc agttatagat atactacctg
     3541 tattaggctc tatttctcct gataatattt ttaaaaaagt agattttccc gcaccattag
     3601 ctcctattat tccatagcaa ttacctttag tgaattttaa gttaacatct ttaaatagtt
     3661 ccttatcacc aaatctaagt ccaacacctg taacttgtaa cattaatata ttttcctttc
     3721 tttttgctca taatttattt tatcataaaa ctttgataac atttttatta tatattatca
     3781 cgtaaatagt atatttttta tactaaattt gtgaaatatt tattaaaata atactaaaaa
     3841 ttgtaaattt ataaaaatta acaatctctt atattatact tataaagaaa tcaaattgca
     3901 actttattca ttcttttaca cattgaaaat aagttattat aatttctaaa taaatatgct
     3961 tttcttaaaa catcattcta gctgttttgt ttcatattta aaaagagtac ttaatttgta
     4021 aatactcttt tttaataact ttatatttta gcaaaattac atgcaggata agtacataca
     4081 tcacagttca tacaaaggcc accatgacca tatctagcta tatcatactt atcaagcttt
     4141 tcatcaatta aaaccctaga taaaactata tcaaatactg tagttttgtg atacattgcg
     4201 catcctggta tacccataat tggaacttct ccataataag caagtaaaaa catagctcct
     4261 ggtaatattg gtgaaccata tgtcactaaa tcagctccag tttcttttat agctgttggt
     4321 gtaacatcat ctgggtctac actcatccca ccagtacaac agataagttc tgctccttga
     4381 cttataaatt cttttattgc actctttata atctctttat catctgggca tatagtctgt
     4441 cctataactt cacaaccaaa gccttttact ttttcttcta ttacgggtcc aaatttatca
     4501 acaattctgc tataaaacac ttcatttcct gtagtaacaa ttcctacttt cttaggttta
     4561 aaaggtacta catttactat ttttctatta ccaactactt tttttgcatc ttctaatttt
     4621 ttctcatcta taaccaaagg tatcactctt gttccagcta cttttaaacc tttatttact
     4681 ataaaattat tatgaagagt agccatcatt atttctccaa cacaattcaa atcaaaaagt
     4741 gcatcaacat ctatctttaa tagaccatca catgctgcta taaaatctat tttcccttct
     4801 tttatttcac taaaatctaa attttctcca gcagttagtt cttttaaaaa caatgcgcct
     4861 tcattttcat gaaccattcc ttctgacttt tcccatacat acaaattatc tttaccaaga
     4921 gataataaca ctggaatatc ttcttctttt actatgtggc cttttttaaa ttttcttcct
     4981 ttaaattctc caggtattat ttgagttatg tcgtgacata aaacttgtcc aacagcatct
     5041 atagttttta ttagcttcat aatttacccc caattctctc atagtccatt tttgtatcga
     5101 tatcaaataa catttcttta ggaacttcta catactttat ttttgatgaa ttttttaaaa
     5161 tggttttacc tttttcatct tctttaagtg ccaacaattc ttctttcttg ctatatggga
     5221 atatgactgg atttccacac atatctttgt atcttggaat tactataaaa tctctatcta
     5281 atttaaaagt atttataatt ttatctatat ataagttatc aataaatggc tggtctccta
     5341 caaagaacat ataaccatca cattttgagc tatttaaaac tcctaatttt atagattcac
     5401 tttgacctat atttgcattt ttattatcta tatattcaaa accatattta ttacacaata
     5461 acaaaacctc ttcatattga cttactacta caacttgatg aaaatcaatc ttgctaactt
     5521 ctctaaatac atgttctatt atagaacagc ctttataatt taataaaagt ttatttgctc
     5581 ccattcttct ggcaaagcct gatgccatta tcactgcatt tatcatatat atcatccttg
     5641 gtatttataa tatattgata gttctatgat ttttataata tctctattga tattatctat
     5701 atatataat
//
LOCUS       DAMKMI010000187         5691 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMKMI010000187 DAMKMI010000000
VERSION     DAMKMI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.193"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            104..1429
                     /gene="cdeA"
                     /locus_tag="PC429_003454"
     CDS             104..1429
                     /gene="cdeA"
                     /locus_tag="PC429_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF3848911.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1524..2318)
                     /locus_tag="PC429_003455"
     CDS             complement(1524..2318)
                     /locus_tag="PC429_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3848912.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2319..3194)
                     /locus_tag="PC429_003456"
     CDS             complement(2319..3194)
                     /locus_tag="PC429_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848913.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3198..3566)
                     /locus_tag="PC429_003457"
     CDS             complement(3198..3566)
                     /locus_tag="PC429_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3848914.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3749..4408)
                     /gene="deoC"
                     /locus_tag="PC429_003458"
     CDS             complement(3749..4408)
                     /gene="deoC"
                     /locus_tag="PC429_003458"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF3848915.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4444..5631)
                     /gene="murJ"
                     /locus_tag="PC429_003459"
     CDS             complement(4444..5631)
                     /gene="murJ"
                     /locus_tag="PC429_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF3848916.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
BASE COUNT         1900 a          893 c          631 g         2267 t
ORIGIN      
        1 ttgttgattt taagctgttt atcgtataaa taaaattttt cttgactata tagttactat
       61 atagcttatt atatatagag agtaataaaa ggaggtaaaa tttatggaaa atttatttac
      121 aagaaaattc actacttttg aatttctaaa atttgtttct cctgcaatta tatccatgat
      181 atttatatct ttgtatacaa taatagatgg catctttgta tcgacattag ttggttctga
      241 tgctcttgct agtataaata ttgtactacc tataattaac cttgtttgtg gatttggaat
      301 aatgatggca actggtggag gtgctatcgt ttctatacgt atgggtgaaa atagacagga
      361 tgaagccaac tctacatttt cttttatagt tttgttttca ttgattgttg ggattttatt
      421 cacagtaatc tcatatttct ttatcaaaga aatatctatt ttgcttggtg caacagataa
      481 gttattacca tattgtataa cttatggtaa agttatgatt ttatgtacac cattttatat
      541 tttaaaattt atatttgagt actttgcaag aactgatgga aattctaaat ttagtttatt
      601 tctatcagtc attggtggtg taacaaatat aatattggat tatgtattta ttaaatattt
      661 tggaatgggt cttttaggag ctgcagttgc aactgctata ggtattattt taacttgtgt
      721 tttaggtatt atttacttct tatctaataa atctacacta aaattaagaa aaccaaaaac
      781 cgattttaga cttataagag atactatgat aaacggttct tctgaaatgg ttacagaatt
      841 atctacagga attacaacat tcttatttaa tgtagtagct ttaaaattag caggagaaaa
      901 tggacttgct gctcttacta tagtattgta tgctcatttt ttaatgacat cagtctatct
      961 aggattcgct gctggagtgt ctccattaat aagctataat tttggtgctg aaaacagtga
     1021 taaattaaaa gaaacattta aacattctct aaaatttata tttatttctt ctcttttagt
     1081 gtttattatt gctttagtat ttgcaccatt tattgttagg gtctttgtaa atccagataa
     1141 cacagtattt aaactagcct tacaaggatt aaaaatattt gcatttgctt ttttgtttgt
     1201 tggtataaat atatttgcat caggattttt tacagcattt cacaatggaa aaatttcagc
     1261 tattatatct tttagtcgtg cctttgtttt tataatcata ggaatcataa ttcttcctcc
     1321 tatgttaaac atgactggat tatggcttac agttccattt gctgaggtta taaccatatt
     1381 tatatctatt ctatttataa aaaaatataa atgtagatat aagtattaaa aattaatact
     1441 taaaatcaac atttattaaa actaataaac ctgctttaaa atacaaattt acaaatttga
     1501 tttttaagca ggttttattt tagttataat aagtcatttg catactcttt aattggtgct
     1561 tttctaagta ataaaattat aaatattatg caaataacta taattcctac ctttgttcca
     1621 aataaaataa aaggattctg aaatgaataa ataatttttt ctaaatacaa tagcatttta
     1681 tgcatatttt tagttgcaaa attaactata taaattacca ttaatatcgg taatcctatc
     1741 aatatagtaa tactagtaac ttttctaact ctatatgtaa aagctccaag taacactcct
     1801 ataaaaccta tacatatttc atttatacat atttgtacaa aataatttaa ataagttagt
     1861 ggcatacttg atgttgaagg atctatcagt gctgatatag atgtccaaat catatctctc
     1921 tttagtaaaa ctgatagttc tatactgtaa cctgttataa tttctataaa taacttcgat
     1981 aaaaaaatca aaactatacc taaaatagaa atcacaaagc ttaaaataat taaatctatg
     2041 acaatagctt ttatacaact ttttctatct gctcttatac ttaaagctcc tgaaaagtct
     2101 ttatttgcta tatttactcc atacacaaat agcagtatag ttatatatgt taaaatagga
     2161 ttcacaatcg tggataaatt agttttagat gcttctccaa aaatcttgag aataaacact
     2221 ggcaaaatat tcataacaat tcccacaatc ataaatataa ttatatacaa tttagtattt
     2281 ttattaaaaa attttatata tggctttaat tcattcaatt acaattcctc cttcttagtc
     2341 atgttaacaa acatatcttg tagccctatg tatcctacat caatatttga atttttaagt
     2401 attttttcat cattttcact aaaatcacca tagtaaaaat aagcaactgc atttccaaat
     2461 gcctttttag gttttatatt ttttatacat tctaattttt ctaactcttc tttatttcca
     2521 gtaatataat gagctttttg ttttatgaca tcaatttctt catctataat aatttttccc
     2581 tcatttaata ttattacatg ctccagtaaa tcatctattt catcaatcaa atgagttgat
     2641 attattattg tccttggatt ttttatataa ctatctaaaa ttatgttata aaactcttgt
     2701 ctatttactg catccaaacc aattgttggc tcatcaaata tggtgatagc tgaattagaa
     2761 caaataccta ttatatttga aagtatagtt ttcatacctc ttgacagttg tttatacttt
     2821 tttttagtat ttaaatcaaa atatttgcat aattcttttt ctaagccttt atcataactt
     2881 ttataaaaac tagaagaaaa ttcaaatatc tgacctactt taaaatctgg actataaaat
     2941 tctttttccc tgacaataca aacttcttcc aaaactttta tatcttcttt tgggttcttt
     3001 cctaatattt ctatctcacc ttcattttga attaactgat ttaccattat atttaataat
     3061 gttgtttttc cagctccatt cttacctaat aaaccatata tcttattttt ttcaaagttt
     3121 agagacatat tattaaaaat attttgcttc ttaaaactgt gagataaatt ttctatctta
     3181 atagaactac tcatatatta atcctccttg aaattaatta taatatcaac caattcttct
     3241 ctatttattt ctaatttgtt agcttcttga agtaggtttt ttacaaaatt atgtttaaaa
     3301 ttttcttttc tagcttcttt tataatagtt tttgcaccat ctgatacaaa catccctatc
     3361 cccctcttct tatataaaat atttttatca accaatatat ttataccttt taatactgta
     3421 gctggattaa ttttataaag ctttgaaagc tctgttgttg atggaacctg ttcttcttct
     3481 tttattccat ttgatagtat ctcatcttca attgctcgtg ctatctgaat aaatataggt
     3541 tcttcattat ttaatactaa tttcataggt tcctcctttc tccattagtt agttactcaa
     3601 ctaattaact atataactta taatatcttg aaaaatattt tctgtcaata ctaaataaaa
     3661 aacagaggga aaattccctc tgttttttgt tctttatatt tataattaaa ttttgatttt
     3721 tgtaatccta tcccataagt taaaaacttt aataatctgt tgtatcttca ttttttaata
     3781 ttttacatat tgagctagtt cctaaccttt ctgctcctgc ctctataaat ttttctgcat
     3841 cagaaattga tttaactccc cctgcagctt ttatttttac attttttcca acatgctctt
     3901 tcataagctt tatatcttct aaagtagctc cacctgttcc cattccagta gatgttttta
     3961 taaaatctga acctgacatt gttactattt cacacatttt tattttttca tcttcatcta
     4021 aataacatgt ctctataatg acttttaata ctttatctcc tatagctttt tttatttctt
     4081 ttatttcttt tcctatatta tcataatctt tattttttat atctgaaatg tttacaacca
     4141 tatcaacttc atctgctcca ttttcaatag catcttttgc ttcaaatact tttgtagcag
     4201 tagtttgata tcccaaaggg aaccctatca ctgtacatat ctttatttta ccttttagat
     4261 attctgatgc tcttttcaca tatgatggtg gtatgcaaac tgatgctaca ctcatatcta
     4321 cagcttcatc acataaaatt ttaatatctt cccatgtagt tgtagctttt aaaatagtat
     4381 ggtctactgt ttttaatata tgtttcataa aatcctccta aatttataat taatataatt
     4441 agattacttt tttaaatgca attttctctt tgcaatattc agtatcatat caacctcttc
     4501 aactttaaaa atagtcatta gtagtgtata aattccacct ccaactataa ctgaaatagc
     4561 tagtgagaca aactcattaa atgttcctac tcccaaaatt ccaaatataa atttatacgt
     4621 aaagtatgat aaaacaccca taattaaaga tgcaactacc gatttcaaag ttgcttttat
     4681 tattttatct tgtccaaaat atccaacttt tctctttagg ttcaagaaga gtaacaatat
     4741 acatgcaatt gatgatgaac tagttgcaag agcaagacca ccatgagcca ttggttttat
     4801 taaaactaag tccaatacta tatttacccc tacagatata atcccattta ccataggtgt
     4861 ttttgtgtct tgtaatgaat aaaaaacctt tcccaaaata tctcttaagc caaatgcagt
     4921 cataccaact gcataaaaaa ttaaggcagt tgctgtcatt tgggttgccc tcgcatcaaa
     4981 agcacctctc tcaaaaatga ttctaacaac aggagttgca aaaaaaatag aagcaactga
     5041 aataggaatc atagaaatta ttatcatatt tatactagat ttaactgaac tagtaaactt
     5101 cttttgatta ttttctgcac ttaacttaga taacatagga tacataacag atactattga
     5161 agctgtaaat gtccctgtaa caaaaccatt taatctatct gcataagtaa gtgctggaat
     5221 gcttccttta actaatgttg aggctaaagt tgtatctacc aatgagttca cttgattaac
     5281 agcaacacct ataaatactg gtgaaagtaa tgctagtaat ttaataaggc tatcatcttt
     5341 aaagtttaag tagtataagt atttgtagtt cgttttttta acaaaaaaca tgtaaaagag
     5401 cagttgaact accatagcaa ccacagcacc aattggaagt atgtatggtc caaatatggt
     5461 acttaacatt attgaaataa ttattactat attatagggt atacttccaa atccaactac
     5521 aataaagttc tctttaattt gtaaaaatgc tgacataaca cttgtaatac ctatgaatat
     5581 tattcctgtt ctgaacctcc catgactaaa gtcacagggt tcctgcttca tagaattttg
     5641 catactaaaa tatgtcttac taattctcca caggctatcc ccgtagttcc t
//
LOCUS       DAMKMI010000188         5670 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMKMI010000188 DAMKMI010000000
VERSION     DAMKMI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.194"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            151..1701
                     /locus_tag="PC429_003460"
     CDS             151..1701
                     /locus_tag="PC429_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF3848917.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            1727..4429
                     /locus_tag="PC429_003461"
     CDS             1727..4429
                     /locus_tag="PC429_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3848918.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQITKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(4935..5600)
                     /locus_tag="PC429_003462"
     CDS             complement(4935..5600)
                     /locus_tag="PC429_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848919.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
BASE COUNT         2162 a          555 c          921 g         2032 t
ORIGIN      
        1 aataggatat aaaaaataat attgtaaaaa aaagtttata tttagtaatg aaaatttaac
       61 gttttacttg aaattatgtg ttttgtaatg tattattaag gctctaatag gaacattttc
      121 atcaattttt ctatagtggg ggtaggaatt atgaggtaca aaaaaagaaa tatagataag
      181 tcaagtgaac tttcatttca atctctagca aaatatttta taactttcta tatagtaatt
      241 atattaatta ttctttctat gttttgtata aatacaatat gggttatgaa aaaagataat
      301 caagatttat tatctacaac atcaaatcgt attgaagata gggtaggaga aacaattaag
      361 ctattgaact ctttgagtaa agataattac ataatagata atagtgtgaa tggcatagat
      421 aaaagtaaag aactttcaat gtatagtgat gaatttgggt atatgatgat atgttttgtt
      481 gataagaata taattgtgtg ggatgaagaa ggtggtaata ttagtctagc tggtcgtgat
      541 tatatgcaga aactttttgc aacaaaaaaa tatcaagtta cagatagttt tgcagcaggg
      601 gctgatggaa aaactttaaa ctacactatt gcatcaccta taataaagaa tggaaatgta
      661 gatggttgtg tatttgcagc aatttacttt gatgagataa aggatatact caaacaagga
      721 aaaggtcaac agcaacagaa tttcatatta tttggaagtg aaaatcaaat aatgaattta
      781 acaagtgatg aaactgcata tggagagaag tatatgaagt tatcgcagaa aacaaaatat
      841 cttggaacca atgcaaaaaa agtagaaaag gatatgttta ataataaatc aggtgattat
      901 tgggcttttg aaaatggaaa attatacttt atgtcctata aaccaattaa gaacacaaat
      961 tggagtttat tttgtaaagt taatttctta gatatttttg cattaaattt aattcctatg
     1021 ggagctattc tatctgttat aactttagta tttattattt tgatttgtat ggagaaaagc
     1081 ttcatgaaaa aacgaatgga agttattgat atgcttttag gttctgttca agaattagaa
     1141 aaacgtatat atcaaacaga aagacctagt gaagtagact ttaaagaaat tattcagctc
     1201 acaagtaaag gtctgactga tggattaact ggtgttgtaa cacgtactgt attttttgac
     1261 caaatttata atcgtgttga aatggaggaa aatgataaat tatctgtaat ttcatttgtt
     1321 gatttagatg atttgaaatg tattaacgac ttatatggac atgaaattgg ggatatggtt
     1381 ttgaaaaatg ttggatatgt acttcgtgaa tatgagataa aatataatgg tttaattggg
     1441 cgctatggtg gagatgaatt tgttatgttg ttatctggtt ttacatcgag agaagaggtg
     1501 gaggatttat tggatgagat tgctattcgc ttacatactg aagtactaac agatgatggt
     1561 atcattcaaa ctcattgtag tgttggagct gtagtatgta gtgtaaaaga caataaagat
     1621 atagataatt tattaaaaca ggctgatgaa gctttatact atgttaaacg taatggaaaa
     1681 ggtaattatc atttagttta atgtatgtgg aaggagttta tatcatatga aaaatgtatc
     1741 taaaaaattt tttgtatgta ctattattgt attaataaca tttttatgtt ttagtaaatt
     1801 tgctatttat gtttttgaca aacaagaaaa gaaagaaaca aaagtagtag aaaacatgtt
     1861 aaatgtctac aagaataaca ttattttaaa tctgactgaa aaatttaatt atagcaaaga
     1921 cttgacatca gttttagttt caaacaatta ttctatagat aaattaaaac ctattgcaga
     1981 tgaaattctt aaaaacaatt cttgcatata tatttcagtt gtagaaaaat ataatgtagc
     2041 tgcatttttt tctaattctg tcaaaaaaga tattatcaat actccaatac aggaactgaa
     2101 ttattcttat tcattagcaa attctatcaa aggattagtc gtagaaggtc cttctaaaga
     2161 ccagttttca aatggagatg tgtttttaat tactaatcct atttataaaa atggagagta
     2221 ctggggagaa attacagtag ctttagatgc caaaaaagtt gtagattctt ttaacttaaa
     2281 taaattaaaa gaatctggat ttgagtatga actttggcgt gtacacctag ataatggttc
     2341 aaaatctaca atagaaattt ctactaaaga ttttaatttt tccaaatcac atgaaattaa
     2401 gtttaaactt ccgactgttt ggactcttag cattgtgtac aaaaatggat ggaataataa
     2461 gaatatagat tttattgtat ttgttatatt gttaattatt tctatattaa taggtgtgat
     2521 gacatttatt tacattgaaa agaagtcgat tagtaaggaa ttaaatcaac agagtcttaa
     2581 aaatttagaa actggtattt tgaatagaga aggtttttta attaagttgc aagagcatct
     2641 gaatagaaaa aataatgaaa tagtgttgct gtatatatgt attactaatt atagtaaatt
     2701 tgctccagtt atgagctttg aaaaaagaca agattatctt acacatatag taaaaaatct
     2761 agataattac ataaaaagtt ctcattttat tgctcgtata agtgaggaag gttttgttct
     2821 agctttagaa gaaaattgtg ataaaaatga attgcttaac attgaaaaag ggatttcctt
     2881 agagctttta cgaaaaatta aagtagaaaa taaacaagta tttttaacga ttggttatgg
     2941 ctcatctagg tgtgttgatg atagaaaaga tgcagaatct ctgttacttt cagctattga
     3001 agattatgag agaagatttc cagcaatgtc tcctattcat gattttacta aaaagtgtga
     3061 agatatgctt gatgaaaaga aacagattgt atttggtgaa tatccagatt tagaaataaa
     3121 caagttgtct aaagtaatat acaaatatta taaaaaagta cagcgtatta tgtataaaga
     3181 ttataatatt gatattggaa ataggcgagg gtatatgcgt gatgtcgaag tattaattga
     3241 ttataattct gagagaatgt tatacttatt tttaatagac atatgcaagt ttggaaaatt
     3301 taatgaactg ttttctattg aaataggaga tgttatttta aaagaagtta gtaacaaatt
     3361 aaaaacaatt ttttctaatt atctatatcg tattaatgga gatgtatttt taggcatttc
     3421 aatagataaa gaatcttact tagatacttt acataaggtt aatgaagtat ttaatagttc
     3481 tttaagtgta gaagatgtac tttttgatgt aaaatgtaat attggtattt gtcaatatcc
     3541 atatcatggt gaaaatccat atgaccttct ggaaaaggta caaattgcat tggagtatgc
     3601 aaaaaaggat gtcaaaagta atgttaaagt ttatgataag gaattgacag aagagattta
     3661 ccatgaaaat aatattatta aggatttaaa aaaaggtctg gaagaacaaa cactagaagt
     3721 ttggtatcag ccaattttta atgttagtaa aaactgttat ataaaagctg aggcactaat
     3781 tagattgaga aataaagagg gcggttatta ttctgcctat gaagttatta cttatgcaga
     3841 aaagcatgga cttattaagg atgtaggtct atatgtacta aaaaaggctt gtgatgtaac
     3901 tacaaaaata aaagataaaa atatagactt tgaacaaatg caattaaatt tatctgctca
     3961 gcagattgta cataagcagt gtgcaaatga catattatca tgtattgaga attgtggtct
     4021 agataaaaag tatatttctg ttgaagttac agaaaaagta cttatggaaa cttttgaaat
     4081 tgtaaatgtt actcttgata aactaaaaaa agaaggcatt agcatagctt tagatgagtt
     4141 tggttctgga tttagtagta taaattatct ggcaaattta cctattgaaa atttaaaaat
     4201 agataaggaa ttagtagaac aaattaccta ttctaaaaaa caatttgaat tattaaaaac
     4261 tatagttcaa attactaaga ttaatggcat ggatattgta gcagaaggtg ttgaaacaaa
     4321 agaaactctg gatttagttg taacttcagg tgtgaatttg attcaaggtc attatttttc
     4381 agaaccttta agtgaagata aatttattga acttatgatt gaagaataaa ggttaataaa
     4441 ataaagattt aaggtattga aaaataatta aaagtattta ataatagaat tagggaagaa
     4501 taagtttata agtaaaaaca attgattagt agttaataaa ataaatgtga tatccaaata
     4561 aatcctaaat ctaatttgag ttttaatata gatagtctaa agtttgatta ttaaagattt
     4621 aattgtaagt tgttacagtt gatgatattg attagagatt aaagtttaga gtagaaagtt
     4681 accttaaatt taaaaatgca agataaccca atctaaaaat tataatcact cgataggttt
     4741 aaggatttat ttgtttatta taaataggag ggtgtctcaa aatgaacttt ttagtttatg
     4801 aggcactctc ttttatatag aattaaatat attttattat ttcaacaatg aaaaagaagc
     4861 ttatctctat tttgagacag cctctttgta tactattatt aatttaatca ataacataat
     4921 ttaaaattat aatactagtt aagatttcct gctttattag taaataattc tttaatagca
     4981 ttgtttctgg aaactaaatc gttatcagag ttgtttgttt ttttcataat cactgaataa
     5041 cgttttttat ttataaaaaa tattaaaatc ttatcatcat caaaattgta ttctttatat
     5101 ctgtcgatag actcataacc tagatttaga atgtttttgt cttttatact atctacatct
     5161 aaaatatatg atgggtcagc ctttagtaat atattaatta aacatttttc ttcaccagca
     5221 cagaataact ccatcttact tgatatagat ttagattgct ctgaagttat ttttgcagga
     5281 ataaggttgt cattatcatc tcttagaaga taaccatgtg tgtcttgttg atatactaaa
     5341 ttaacctttt tcttaggaac actaatcatt ttatctaaat gtacataatg atgattttca
     5401 acaaaacctt catttaatat ttcaacaaag ttattaattt tacctaggca aaaagattct
     5461 atagattctt tacagaattt atttgtaaat ggtattatgt ttacgttttc ctcgtctgta
     5521 ttccatgcta tagcataatc caacgaatat acttcttcct ctatatctgc aaaattatat
     5581 tgtactatgt ttcctttcaa aattactcga cctccatctt ataatatatt taatatactt
     5641 tataaaactt atattatatt taaataatat
//
LOCUS       DAMKMI010000189         5527 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMKMI010000189 DAMKMI010000000
VERSION     DAMKMI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.195"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(510..1061)
                     /locus_tag="PC429_003463"
     CDS             complement(510..1061)
                     /locus_tag="PC429_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848920.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1252..2214
                     /locus_tag="PC429_003464"
     CDS             1252..2214
                     /locus_tag="PC429_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF3848921.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2509..4353
                     /locus_tag="PC429_003465"
     CDS             2509..4353
                     /locus_tag="PC429_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF3848922.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            4675..5259
                     /locus_tag="PC429_003466"
     CDS             4675..5259
                     /locus_tag="PC429_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF3848923.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSLDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2146 a          546 c          875 g         1960 t
ORIGIN      
        1 aaaagtaagc ttatgtgtta tttgtagtaa atataaagtg catatagtga taaaacaaaa
       61 gtttcatata aatgaagaaa aaaatataaa ataatttatt taactactta aaaataaact
      121 aaaagggata ataaaatagc ttttagttta tggtaataga ggtatttaaa aagagtgcat
      181 aagcactcca attgtgagaa atacatacac aaatcattat tacaacaatg aaaaatgtac
      241 atataagaga cgttttgtat ttgttttaag tgataatatt ttaatactaa ctaaataatt
      301 atcacaatgg taaaaaagaa aaaggagtat tttgtactcc atttgtcaaa aatataaaac
      361 ttttatataa gcttattata acataattga taaaagtgta aagatatata atgaatttat
      421 aaagaaaaat aaaataaaaa agataaatcc taatcaaagg atttatcttt ttgtatagaa
      481 aataattatt ctattattaa atggcatggc taaactggat aataatttat tattactgct
      541 ttcaatgttt tttctccttg attatggtat gtatgaattg attcagctga aaaacaaata
      601 gagtcatttt ctctaagttg ataaatagag tcatttactt ctaattttaa ttgaccttct
      661 ataatcataa tatattcctg tgatttttca gaatgaccaa cagatgtata actatgacct
      721 tcttctaatt ccatttgaaa taattcgaag tttctagttg gtgtattggg ataataacaa
      781 taaagacgat aatctttatt ttctgaaact tgtacatcta tatcagtttt tgatacaata
      841 aaagtttcat tttgaggttg ttccagtatt gcagtatatg gaacatttag tccacttgca
      901 attttccaaa ttgtgtttac tgttgggttt gagtctccct tttcaatttg agaaagcatt
      961 actttactta cgccagataa ttcagcaagt tgacctagac ttaagttacg ttcattacgt
     1021 aatctattta aatttttagc aataacagta ttaatactca tagtagctcc ttaaaatgta
     1081 aattattgac aaatataatt tacgaatgta taatttattt aacattattt ataatttaca
     1141 aatgtataat ataatgaacg ttaaatacac tgtacatccg taatataaat tatactgcat
     1201 aaagagttgt ttttcaacgg atgatagttg agagattaag ggggaattgt aatgcgtgta
     1261 cttaataatg ttacagaggc tataggcaat actcatatgt tacatttatc caaaatagca
     1321 gatgaatttg gagttcaagg taatatttat gcaaagatgg aatacttaaa tccgggattt
     1381 agtaaaaagg accgtgtagc attacaaatg attaccgaag cagaaaatgc aggtttatta
     1441 gttccaggtc aagcagttgt ggaattaaca agtggtaata ctggaacagg actagcaata
     1501 gcttgtgcat gtaaaggata taaatttata gcgtgtatgt caaaaggaaa ttctatggaa
     1561 agagcaagaa tgatgaaagc tttaggtgct gaagtagttc ttgtagacca aatgcctaat
     1621 tcagttcatg gacaggtatc tggtgaagat ttggcacttg tagaaattga ggctcaaaaa
     1681 atagcaaaag aaagaaatgc atttagagca gaccaattta atttagaagc ttgtaatcat
     1741 gcacatgaaa agtatacagc agaagaaatg tgggaacaat tggatggaaa tattgatgta
     1801 tttgtagatt ttcttggaag tggtagtact tttgcaggat gttcaaaaac acttaaaaaa
     1861 cataattctt ctatcaaatg ttatgttgtt gaacctgaaa cagcagcaat ttattcaggt
     1921 aaagaaataa ctgaccaagg gcataaaata caaggtggag gatattctat ggatttgcca
     1981 ttagtagaca agaaacttat tgatggttat attcaagtta cagacgagga ggcaactgat
     2041 gtagcaagaa aattagctaa attagaaggt gtatttgcag gattttcttc aggggcaaat
     2101 gtatgtgcag caattaagtt gttaaagagc agtgaaaaag gaaaaaatat agttttaact
     2161 cttaatgata gtggtttaaa atatttaagt actgatttat atgaagaatt gtaataatag
     2221 cacaatagtt atgcaatgtt ttttaaatta agtatataga tttaaattat aaaaagtatc
     2281 tcaatgtaaa ttttgaaagt tataaaggag atactttttt attttgtaaa tatggagtat
     2341 cctacttata agtattaagc tatatagata ttgatgatta gctatgcaat aaaaaagagt
     2401 atgtgttttt gcatttatac atcataactt ggaataatac atattaatgt tctaaatgta
     2461 tttaaaatat atgataaaat tagaacaaat agatacaagg agataaaaat gcaaaagaga
     2521 attttaagaa attttatatt tacgggtaaa aatattttaa gtaaacttgt ttttataata
     2581 aaatataatt ttagtacatt aattttattt gaattatttc ataaaggatt agccttattt
     2641 tttatatggc catctattag atatatattt gatattttaa taaaaagttt acatatagtc
     2701 tacctaaata tggataattt tataaaagtt ttgaccaatc ccatgtcaat agttctgata
     2761 atactatcag ttataatact agctttttat gcattttttg aatttacctc tgtaattatt
     2821 tgttttaata aatcgataaa atatgaaaaa atagggttat ttgaactctt taaaatatca
     2881 tttgagaact ctataaaatt gttatatcct aaaaatatac tattatttat atttgtgata
     2941 ttaataattc cattagtaaa tacagtttta atttcagggt ttataggaaa aatacaaata
     3001 ccagagtaca tattagatta tataaagtct gatataattt taaatataat ctatatgatt
     3061 tttcttttaa taatatatat ttcggttatt agatggattt ttagcattca tgagataact
     3121 ttaaatacag aaaattttaa aagagcaaga aaagaaagtg ttaatcttat aaagggaaag
     3181 attataagaa caattattta ttcattaata ttatctttag tagtagctat tattggatat
     3241 gcgatttatc atagtggaat tatatttata ggcatatgga ctaaatattg tactgatatg
     3301 gaatttttga agaatgtatt tataaataga tgtattgcat ttgaagaata ttttagattg
     3361 attttatcaa tattcatttt tatattggac attggattta tctcagcaac ttattatcaa
     3421 tataaaggag taaatatttc taaaaataat attaaaaaag agaaaaaatc tatatcaaaa
     3481 attgttttta atttagtatt aatattattg atttcttata tagagtcagt tgcattttct
     3541 tcacatataa atggtatgtt taatacgtca ttttcatttt atacaacagc aattgctcat
     3601 agagcaggag ctgaaatggc tccagagaat actttggcag caatagaaga agcttcacat
     3661 gctaaagctg attatgcaga aatagatgtt caagaaacta aagatggaga acttattgta
     3721 cttcatgatt caaactttaa gagaactaca ggagtagata aaaatgtttg ggatgtaaat
     3781 tataatgaag taaagacata tgatgcggga agcttttatt attatggaaa aggttattat
     3841 gatgaaaagt ttgttgggga aaagatacca acactcaaag aaatgataaa atattctaaa
     3901 ggtaaggtga aattattaat agaaataaaa cttaatggac atgaaaaggg agacgtagta
     3961 aaaaaagttg ttcagttaat taaagaaaat aattttgaaa atcaatgtat agttgcatct
     4021 atggataaaa ctatattaca aaaagtaaaa aaactggatg agaatttact gacatgctat
     4081 tttatggcaa ttgcatatgg tgatttttca aagttaaatt atgttgatat atatggaatt
     4141 gaatcaactt ttgttaataa aacagtagta gaaaagattc ataaaatgaa caaaaaaatc
     4201 ttcgtttgga ctgttaatag tgacagttta attgagaaaa tgcttgacct aaatgtagat
     4261 agtattatta cagataatcc ataccttata gagagtgcaa tttattggaa gaaaaatgga
     4321 tttattagtc aagtgtcaga ttatttattt taaatattct ataattgtat tatccagtaa
     4381 aagagatatt attaaaatca ataatatctc tttttaacat aaataaggtt gttaaattaa
     4441 ggctattctt ataaaaattg tgtttatcag atataaataa ggaataaaag taaaaaaatt
     4501 gctatgacat tattacaatt aaaagagttt taatatgata gagaggtaga ttgtcaactg
     4561 tatatttaat aaattttatt atttattaga ttttatataa gtaaaatata tagatatata
     4621 aatcttatgc tagaatatat atgaaaaaca atatagattt ggagggttaa aagcatgaca
     4681 gagcgagaaa aaatgttggc aggagaactt tacgattgtg gtgatattga gttgttaact
     4741 caatggcata aagcaaaaga cctagtaaga gattataacc aaacagattc cttggatttg
     4801 gaaaaaaaag aaaaaatatt aaatgagtta cttggtggta agggtaaaaa cttatggatt
     4861 actgcaccgt attatgtaga ttatggaaat aatatttatt ttggaaataa ttgtgaagtc
     4921 aatatgaatt gtacgttttt agatgataat aaaattatca ttggggataa tgcactaatc
     4981 gcaccaaacg tacaaatata tactgcattt catccaacta atgctggtga tagatttggt
     5041 aatgccaaag aagatggttc atttgaattt tgtaaaactc aaacagctcc agttactatt
     5101 ggagataatg tttggattgg cgggggtgct attatcatgc caggtgttac tattggagat
     5161 aatgtagtta taggtgcagg aagtattgtt acaaaagata ttccaagtaa tatgattgca
     5221 tacggaaatc catgccgtat tattcgagaa aataaataaa gttaaatttt cttgcaaaat
     5281 ctcaaaaaac acctttctta ctttatacat agggtatttt ctaatacttt cacatccata
     5341 ttcatttgtt aaatttttgt ggaatgtagt ttttatcact tctatcttac atttccacca
     5401 tttttttata aaagtcattc ctttatagat ttatttataa tagtatatat tctatttgat
     5461 gtttgaataa gcacttattt ttaagtaaaa tactaacttc taggttagta ttttactata
     5521 gtatgat
//
LOCUS       DAMKMI010000190         5519 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMKMI010000190 DAMKMI010000000
VERSION     DAMKMI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.196"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            44..160
                     /gene="rrf"
                     /locus_tag="PC429_003467"
     rRNA            44..160
                     /gene="rrf"
                     /locus_tag="PC429_003467"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(374..1558)
                     /locus_tag="PC429_003468"
     CDS             complement(374..1558)
                     /locus_tag="PC429_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF3848924.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1931..4282
                     /locus_tag="PC429_003469"
     CDS             1931..4282
                     /locus_tag="PC429_003469"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF3848925.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4304..4843
                     /gene="nrdG"
                     /locus_tag="PC429_003470"
     CDS             4304..4843
                     /gene="nrdG"
                     /locus_tag="PC429_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF3848926.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAKEAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKQVFLVEQYMKDDLSIAE"
BASE COUNT         2150 a          624 c          888 g         1857 t
ORIGIN      
        1 aaacttatta aaaacttaat agaaatatta agcataaaga ttatgtggtt acaatagcag
       61 agaggataca cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac
      121 ttggtgggaa actgcctggg agagtaggac gtagccacgt aatttttttg ttataataaa
      181 tttagaattt aacttatata gtttaatttg ataatttgat gaaaaaaata aaagacttat
      241 tgatttttaa atctataggt cttttatttt ttatctaaca tttaaattaa gttgttgaat
      301 atatcttata ttacttacct tatatattaa ttatcattaa tacattatat ctaattacta
      361 ttcatctaaa ttattatgcc attattttat atacaaattc ttttaattta tctaatccag
      421 ataagaaagt attttcactg caagcatagg atatacgaat atatttatcc ataccaaatg
      481 ctatgccagg aacaactgca acattatatt cttctaaaaa ttgattacaa aattctatag
      541 aaaaactatc tttatattca aatttctcag atactttaga aagatctata aatatataga
      601 atgcgccatt tggattcaca tatcctacat tatcaataga gtctagtttt gatttaatta
      661 aatctcttct tgatttatat gttttaacca tattgtctat gtcaattgag caatctttca
      721 aggctccata ggctatgtat tgtgctgtta gacttgggtg agatataaga tgtccttgta
      781 tagaactcat tgctttagca atcgttttat tagatgcagt atatcctaat cttaatccag
      841 tcattgcagc tgacttagaa aaaccattta tagttattgt tatatcttta gcttcttcgc
      901 ttaatgatgc aattgatgta aattcaccag tgtagcatat tttttcatat atttcatctg
      961 ctaaaatata tattttattt tgtatgcata catctactat ttctattaat tcatcttttg
     1021 tataaacact accagtagga tttgaaggat tgtttattac aagaattttt gttttatctg
     1081 ttatagattt ttcaagttct tcttttgtta acttaaatcc attttctttt ttagtatcaa
     1141 taaaaactgg aacagcatta actaacttaa tcatttcagg ataactaacc caataaggct
     1201 ttggtaataa gacttcatct ccttcgtctg ttaaagctag taaagtattt gttattgagt
     1261 tttttgctcc acttgataca actatttcgt ctattgaata gttacagtta ttttcttcaa
     1321 taagtttttt acagatttcc tctctaagta tttttaatcc tggaactaaa tcatatttag
     1381 tgtaatcttt atttaaagaa tcaataccat aagattttgc gttattaggt acattaaaat
     1441 cgggttctcc tatactaaga tttattactt taactccatt actttccatt tctttaactt
     1501 tagaactaat acctattgtg taggatggtg ttataaagtt tagtcttttt gataacataa
     1561 aatgcctcct tttaaattat tatttatatt atacgacaaa tttaaagaat ttacataagg
     1621 aaatttgtag atatatacat taatatatat aatagcctac actttttgcc taaaattagt
     1681 gtaaaaaaaa gagaaaaaaa ttttaacttt caaagttgtt agaaacgcta ctgttcaaaa
     1741 tatccacata gtatttgcaa tgatttgcgc aagtttgact attatgtaca aatttggtaa
     1801 tcttaatata gcaattatta agataatccc tttaacttag ttgaagggga tttttttaga
     1861 cttaacatac tacatatagt ggtatgggga ttaataaaat acaatatata gtaacaggag
     1921 gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga agagtaattc catttaatga
     1981 agatagaata acaagagcta tatttttagc agcaacgaat gttgcagaaa gagaaggaat
     2041 agtacctgat tataaattat ctgagcaatt aactcaagaa gtaataaagt ttttaaatca
     2101 taaatactca gaatcagttc caagtgttga agatatacaa gattctgtag ttaaggttct
     2161 tatagaaact ggccatgcaa aaactagtga agaatatata atatacagaa ctgaaagaag
     2221 tagaataaga aattcaaaaa caaggttgat gaaggcaata gaggaaatta cctttgaaga
     2281 tgcagaagat gctgatataa aaagagaaaa tgcaaatatt aatggtaata cagcaatggg
     2341 aactatgtta caatatggaa gcactgtatc taaagaattt tgtaaaacac atatattaaa
     2401 accagagcat tcttttgctc atgataatgg agatatacac atacatgata tggatttttt
     2461 aaatatggga acattaactt gttgtcaaat agatgttaaa aaactattca atggtggatt
     2521 ttcaacagga catggctttt taagagagcc acaagatata ataagttatg gtgcattagc
     2581 tgcaattgct atacaaagca atcaaaacga tcaacatgga ggtcaaagta taccattttt
     2641 cgattatgga ttggctgaag gtgtatataa gacatttaag aaattctaca taggaaattt
     2701 agctaaagca ttaaagttat ttaaaggaat tgaaaataat gatgtgataa agaacatagt
     2761 atataataca gagaaagaaa caaatcaaaa agttggtctt aagagagatg aattgtatct
     2821 aaacttagaa aaagagaaat taatacagac ttttgatata gatgatgaac ttgtaaataa
     2881 gatgcaaaac tttgcatttg aagaatcgta tagagaaact gataaaaaaa catatcaatc
     2941 tatggaagca tttatacaca atctaaacac aatgcactct agagcaggtg cacaggtacc
     3001 attttcaagt gtaaacttcg gaacagatac ttctgaagaa ggaagaatgg taactaagaa
     3061 tcttctatta tctcaagaaa gaggtttagg taatggagag acaccaattt ttccaatttt
     3121 aatatttaag gtcaaagaag gcataaattt aaatcctgaa gaccctaatt atgatttatt
     3181 taaattatca tgcagagtat cagctaaaag actattccct aattttagtt ttttagatgc
     3241 gccttttaat gcaaaatatt ataaaaaagg tgaaccagat acagaagcta catatatggg
     3301 ttgtagaaca agagtactta gtaatgtgtg tggttcagaa acagttagtg gtagaggaaa
     3361 catatctttt actacagtaa atttaccaag attgggtata aaacatggta ttataaataa
     3421 tgaaaaggct aatttagacg gtttctttga agaattagat gagaagataa acttaattat
     3481 agaacaatta ttggagcgcc ttgaggtaca aggaaataaa aagatgaaga attttccatt
     3541 cttaatgggg caaggtgtat ggaaaggttc tgatgattta ggaccagaag atacactaaa
     3601 agaagtaata aaacaaggaa cattgacaat tggatttata ggtttagcag agtgccttat
     3661 tgctcttata ggtaaacatc atggagaaag taaagaagct caagaattag gattaaaaat
     3721 agtgtcacat atgagacata aaatggatga agcaacggac aaatataaat taaatttctc
     3781 cttaatgggt actccagcag aaggtctttc aggtaggttt actaaaatag ataaaaaagt
     3841 ttatggagaa attaaaggta tcacagataa agaatattat actaattcat tccatgtacc
     3901 agtttattac aatataagtg catatgataa aattgagata gaagctccat accatgaatt
     3961 gactaatgca ggtcatataa catatgtaga gttagatgga gatccatctg ataatttaga
     4021 agcttttgaa actgttataa aagctatgaa agatttgggt ataggatatg gaagtataaa
     4081 tcatcctgta gatagagacc caatatgcgg tttttcaggt gtaataacaa gtaatatatg
     4141 ccctgtatgt ggaagaaatg aagatgaaag tgatatcaaa ttcgaaagaa taagaagaat
     4201 aacaggatat ttagttggta cagttgatag atttaataat gctaaaaaag cagaagtaag
     4261 agatagagta aaacatagat agttttattt gggggaataa gttatgaaaa taagaatgtc
     4321 atctactata agttatgaca gcatagttga tggaccagga ttaagaatgg taatatggac
     4381 ccaaggatgt attcataatt gtaaagagtg ccataatcct cagacacatg atttatgtgg
     4441 aggattttat atggatacag aagaaattat aaataaagtc aaatcattga aattacaaaa
     4501 aggcattacg ctatcaggag gagaaccatt tttacaacca gaaccattag aagaaatagc
     4561 aaaagaagct aaaataaatg ggttagatgt atggtcatat actggattta cgtttgaaca
     4621 actgttagat aaaaaaaata gagcgtattt taaaaattta aatttactaa agcaaattga
     4681 tattttagtg gatggaaaat ttatagctga aaaaaaagat ataagcttaa agtttagagg
     4741 gtcatcaaat caaaggataa ttgatgttca aaaaagtttg aaatataaac aagtgttttt
     4801 ggttgagcaa tatatgaagg atgatttatc tatagcagaa taaaatataa ataaacagga
     4861 ttaaatatat gcatatattt aatcctgttt attttcatat aatatttcaa gcttgtataa
     4921 tgtaacaatt aaaccaaaag actcattgta tatttgaaat taagtttttt atagtttata
     4981 ggtgttttat gtggattacg aatgatatag tattgataca ttatttaata gattcttatg
     5041 ccaagaaaca attaatttat agaaattctt aattgttaca atataaatat ctagatatgt
     5101 tataagatac tgataaataa tttataaagt ttattaatct aatattgtgt gtgaatatta
     5161 agtggtcaag tagtctaaaa aatgaaaaag atttaaaaaa gtgttttaac taaacacata
     5221 agtgttaaat tcatgtataa gtataggtta gatacataag aaataattat gtaatatcta
     5281 tctttgtaaa aaaatattaa attttgtaat ttgtggataa atatgtttat aatgtggata
     5341 atatttaatg taataagtta aagcaatatg aatcaactgt ttataatgtg gataatatta
     5401 taaatgtatg aataattgca gtatttaaaa acaaaaaaag ttaaaaaagt tgttgactct
     5461 aaaagaaaaa gttggtatag taatacttgt cctcaagaaa agggcaaaca aataaagca
//
LOCUS       DAMKMI010000191         5491 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMKMI010000191 DAMKMI010000000
VERSION     DAMKMI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.197"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(188..871)
                     /locus_tag="PC429_003471"
     CDS             complement(188..871)
                     /locus_tag="PC429_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF3848927.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAVIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
     gene            complement(1047..1637)
                     /gene="zapA"
                     /locus_tag="PC429_003472"
     CDS             complement(1047..1637)
                     /gene="zapA"
                     /locus_tag="PC429_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF3848928.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            complement(1662..4055)
                     /locus_tag="PC429_003473"
     CDS             complement(1662..4055)
                     /locus_tag="PC429_003473"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF3848929.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            complement(4072..5091)
                     /gene="pheS"
                     /locus_tag="PC429_003474"
     CDS             complement(4072..5091)
                     /gene="pheS"
                     /locus_tag="PC429_003474"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF3848930.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     misc_binding    complement(5141..5375)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1669 a         1081 c          599 g         2142 t
ORIGIN      
        1 tctatatatt gcctactata cttaagacat tttaattaat ttatacactg cttactatac
       61 ttaaaatact tctattaact tatacattgc ttactatatt ttaaattctt taattaatct
      121 atgcaatatt tgctatatat aaagtatatc aaataacagc ttcatttttt tgtaaattta
      181 cagaaagtta attttcatta aatgaatcat ctttattgat aaacataatt ccaaagacca
      241 ctgccaaaat aaaaaaacat attagtattg atttttccac gaaaattttt gtaaaaaaag
      301 ttcctaaaat agctccagat ataaagaata taataacagc ataatactgt aaactctttt
      361 ttatcataaa aatattctta gtatgtatat aatgaaataa agtttctgta gcacttctta
      421 aatttccagt acacattgtc gttgcaaaac tattcccatt tacttttcta aaactctcta
      481 cttgcattga acaaataaaa gaaatagcaa catttacaaa catattaaat cttcctctag
      541 gaataaatga aacaataagt attgtaatca tttcaattat aatgataacc tgacgccaat
      601 gaatctttgt cttattattt gtaaaatact ttcttatctt ctcggctata aatacaccta
      661 acataaatgc caaaatagga atcaaataat aaaatgattc ctttacatta ccttctgcta
      721 tattaagccc aaacaaaact atatttccag tttgtgcatt agcaaagact ttacctctta
      781 ccacataagt ataaatatcc aaaaatcctc ctgttattgc caacaaagaa cataaaaaga
      841 atgattcaga catctgactc ttgcttgtca tatataaacc tctcgctttc taatgataga
      901 tacaaaatca aaatcatttt agattctatt tttcattgat ataaaaaaat tatttatatt
      961 taaatattgt ctatatttat acatcattta tatttataca ttaaaagaag ctgtctcaaa
     1021 aaacaagaga cagcaaatct tttagtttat tccacatttt ttgcatcatt atttaaccct
     1081 gtacatttta actgtaaatc ataagctttg ttttgaaact cagaagctaa gttttcagct
     1141 atagtagctc tttcatttgc ttcttcaagt tgagtactta actcctcaat cttgttttta
     1201 taagcatcta actctgctct agaaccttct gtcgtattac taagttcgaa tatttcttgc
     1261 tttttatttt ctataatctc aattaactct ttcatcttag tttcagcttc acgattttct
     1321 gcttcttttt ctgctatcgt aagctttaaa gacttgattt ctaattgaag ctcttcatta
     1381 gatgctccaa ccttcttact caattcttca ttagctttaa tcaacttctc attctcatct
     1441 gaacattcaa ataaaaggtc tgatatattt aatgcagtta atatcgcagc aactgataag
     1501 ctcaatttcg ggttttgacg tgttatttta tccatttctt tgtctacaaa atcagcaata
     1561 ctaatcataa acttctctga tttgtctcca accattggat attcagcgcc atggatttta
     1621 accattactt tgttcatcct aacctccccc gacattagtt gttaatttga tcttaagtta
     1681 gcatttaatt tttcgcttaa ttcacttaat atcttatcat gcaccttagc tacatcttca
     1741 tctgttagag ttttatcttt acttctataa gttattgaat atgcaattga cttgtatcca
     1801 gcttctattt gagaaccttt gtaaacatca aataacttgt agctttctac taatccttga
     1861 ccattttcct ttattatatc ttctatttgt tttacaaata catcatcttt aacaataaga
     1921 gctatatctc ttgatgtaga tggatatttt ggtaatggca cataattctt tgttctatca
     1981 gaattatcaa atacaaagtc aatatctatt tcagcgacat aaactctttg acctagattg
     2041 tagttttcta taacatctgg atgcaattca cctaatgtac ctacatattt attattatac
     2101 actattttag cacatctacc agggtggaag gtagtattat tatcttgagg ttcaatttca
     2161 taacctttaa atccgacatt tacaagtata gtttctatag ttccttttag tgcaaagaag
     2221 tcaacatctt taccatacat acctacacac attctatttt cttccttagg taattctgaa
     2281 tcttgtggga tgaatatatg tccacactca aatgcagata cttcttctat tttatgagat
     2341 acattagttg ataaaacatc taacatattt ggtattaaag tagttctcat tacagaagtt
     2401 tcttctccta atgggttaag taatttaaca aaatttctct ttgcattacc ttctggtact
     2461 cttatcttat ctactccctt agggcttaca aatgaatatg ttaatatttc atttaatcct
     2521 acagcagttg aattatcttt taaagcatcc atgaattttt gttttgaagt tttaattcct
     2581 gctgtcgtat ttccttctaa ttgtacagaa ggtatattct caaatccata aattctagct
     2641 atttcttccc atacatcagc ttcttgttcc atatctgttc taaagcttgg tacatctatt
     2701 tctaatttat cattagctac caaattacat ttaaactcta atgattctaa aattcctaca
     2761 aactgctcca ttggtacatc tacacctaat agatggttaa ttctttgagg atttactacc
     2821 aattttttag gttctggttt atttggatat acatctacaa caccttttaa aacttttcct
     2881 gctcctaaca tttcaacaag ctgtgcagct ctatttgctg ctatctctgc aagattaggg
     2941 tctaagtctt tttcatttct tgaagatgct tctgacctaa taccaacttt tttagctgtc
     3001 attcttatgt tttctggttt gaaattggca ctttcaaaaa gtacagtctt cgtattagaa
     3061 gttatttctg agttagcacc acccattaca ccagctaaac caagtgattt ttctccattt
     3121 gttataacta gcatatcttt gtctaatgtt ctctcaacat catctaatgt tacaagtttc
     3181 tctccatctt tagcatttct tactactatt cttccagtct ctacttgatt tatatcaaaa
     3241 gcatgaagtg gttgaccaag ctctaacatt acgaagtttg ttatatcgac tatgttactt
     3301 ataggtctta ctcctgcttc tgtaagtctt ctttgcatcc aatatggaga aggttctatt
     3361 tttacatctg taaccattct agcaacatat cttctacata aatctggatt atctatctca
     3421 actttgaaat ctatctcttc gtcactttct tttacttcga tttcaggata ttttactttt
     3481 gttcctatag ttgcagctgc ttctctagct atacccatca tgcatttaca atcaggtctg
     3541 tttgaagtta attcaaaatc tattaaagca tcttttaatc ctaaaacatc ttttatatct
     3601 tttcctaatt cataagaatc ttcgtggtct aaaatatata taccacctct tttatactcc
     3661 tcaatgtaat gttcatctat acctagttca gctgctgaac acatcatacc atctgataat
     3721 tcacctctga aatcagtctg tttgatttca actcctccag gtaacttaga accatttaca
     3781 gctactggaa tataatctcc ttctgatata tttgtagctc ctgtaactat ttgaacaact
     3841 ttatctccaa tatctacttt ggttacaacc aacttatcag catttggatg ttgttttatt
     3901 tctaaaatct ttccaaccaa tatattttct atttcttcac cataataatc tattgtttca
     3961 actttagttc ctgtcattgt cattttatca gcgaactctt ttacatccat gtctatatca
     4021 acatagtctc taagccattt taaagatact aacatctaca taccctccta attaaaattg
     4081 atttaagaat ctcatatcat tttcgaataa taatctaata tcatctattt cgtatttaag
     4141 cattgcaagt ctttcaaccc caactccaaa tgcaaatcca ctgtaaactt ctgggtctat
     4201 tccacaattt ctaagcacat ttggatgaac catacctgaa cctaatattt ctatccaacc
     4261 ttcatattta cacattgggc aacctttacc accacatttg aaacaagtaa catcaacctc
     4321 tgcacttggt tctgtaaatg ggaagttgtg aggtctaaac ttagttttga tatcagaacc
     4381 aaacaatttt tcaacgaaga tatccatagt tcctttaaat tctgccatag taacatcttt
     4441 tccaacaaca agcccttcta tctgatggaa cattggtgag tgtgtagcat ctggcgagtc
     4501 tgacctaaaa catctacctg gtgcaattac ttttattggt aactcttgac ttctcatagt
     4561 tcttacttga actggagatg tttgagttct aagtaatacc ccatcattga tatagaatgt
     4621 atcgctcata tctcttgatg gatggtcttt aggagcgttt aatgcgtcaa agttgttttc
     4681 aacagtctca acttctggcc cttctgctat agagaatccc attccgataa atatatctgt
     4741 tacttcatct ataatttgag ttattggatg cttcttccca acctttaaaa cttttcctgg
     4801 ttgagtaaca tctatcactt cctcttttaa ttttctttct ttttcaatag catttatttc
     4861 ttctttttta gaatttatgc taagttcaat gttttctctt acctcattgg caaccttacc
     4921 aactactggt ctttcttcag cagataattt acccatttct ttaagtatgg cagttatctc
     4981 accttttttt cctaagtact taactcttaa actttctaca ctttctatgc tttgtgcttc
     5041 ttttatttca gccaaagctg cttcacgtaa agcaagtaat ttttcttgca cacatatcac
     5101 cctttcgttt ttttgtataa aaaaagcccc tcaatccctg tctagggacg aaaggctata
     5161 gtttcgcggt accaccctag tagtttaaca atttaaaaat catcaaacca cctctaacaa
     5221 acttaacgcg tttttgacgt caaagcctac tgtaaaattt cagctctgtt actccagagg
     5281 gaacttctca tgacctctta gaaaacactc ccagcctagg tgtttatctc tttactaagt
     5341 ttaatcaaaa tactttcctc tttcattgca tttacaatat tatattgttt attttagtat
     5401 tatatcattt ccattcaaat tatgtgacta tttttaatat atatccaata cataattaat
     5461 ttaattattt tttatataat atacatctaa t
//
LOCUS       DAMKMI010000192         5387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMKMI010000192 DAMKMI010000000
VERSION     DAMKMI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.198"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            73..909
                     /locus_tag="PC429_003475"
     CDS             73..909
                     /locus_tag="PC429_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3848931.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1262..2839
                     /locus_tag="PC429_003476"
     CDS             1262..2839
                     /locus_tag="PC429_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF3848932.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGIANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2832..4016
                     /locus_tag="PC429_003477"
     CDS             2832..4016
                     /locus_tag="PC429_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF3848933.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVIINNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPTGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4034..4633
                     /locus_tag="PC429_003478"
     CDS             4034..4633
                     /locus_tag="PC429_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF3848934.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4657..5127
                     /locus_tag="PC429_003479"
     CDS             4657..5127
                     /locus_tag="PC429_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3848935.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2012 a          562 c         1027 g         1786 t
ORIGIN      
        1 aatattgaca ttaaattaaa tatactatac tatgtaagtg tgatgagaga atttttttag
       61 aggggattta tagtgaatat aaaaaagata tttaataaca atgtagtagt aagctcccta
      121 gaagatggca cagaaataat tgtcactggt gctggagtag gatttaagaa aaaagtaggc
      181 gatttgatag atgaaaattt gatttctaag aaatattttg ttcaagacga tcaaagggac
      241 aaatacaatc aaatacttaa taagacctct attgaatact ttaaaatttc agaagaaatt
      301 atagaaaagg ctaatgaagt tttaaatact caagtgaatg attcaataat attagcctta
      361 actagtcaca tagaatttgc tgtccaaaga gaaaaacaag gtataaaatt accaaactta
      421 atattgaatg agacaaaaca actttatagg gaagaatttg aatttggatt atgggcaatt
      481 gatgaaattg aaaaaaggat aggaataaaa ttgcctgaag atgaagcagg atatattgct
      541 attcacataa tcaatggttt ggaaaattct caaaaagatg aaggtattaa tatactagag
      601 ttttcaaaac aagttattca gataattgaa gaagtacatg gatttaaatt ggatgtaaat
      661 tcattaaatt atactagact tataactcac ttaaagtttt ttgttcaaag gattctccga
      721 aaagaaactt acaatgatag tgatattgaa gatatgtata agttagtgaa taaaaattac
      781 aataaaccta aagtgtgtac tgaagaaatt gcagcattgg tattagacag atttgaatat
      841 aagattagta aagaagaaga attatattta atgattcaca taaataaaat aacaaacagt
      901 ggttgttagg attgtaactg ataaagcagg caaaacctaa gtcattgagc ttaactatta
      961 gattaagttt tttgatttag gtttttttat tagatacaat caaaatacag acagtgactt
     1021 ttaggattgt aactggttaa gcaggcaaaa cctaaatcat tgaatttaac tattaggtta
     1081 agttttttga tttaggtttt tttattagat acaatcaaat acagacagtg acttttagga
     1141 ttgtaactgg ttgagcaggc aaaacctaaa tcattaaact taattattaa attaagtttt
     1201 ttgatttagg tttttctata aatttgatta tgtttttaga taaaaatagg aggggaaaat
     1261 tatgaaaaaa aataagttag gtttatggaa cttcttccaa atgttaggaa aaacgtttat
     1321 gttcccaata gcactactta gtgtttctgg gatgatgcta ggacttgggg caggttttac
     1381 agaccctaag atgattgaaa tggttccatt tttagggaat gaatatgtta acttagtttt
     1441 aaacttcatg ttaacaatag gattatttgc atttaacaac ttgggagcat tatttgcaat
     1501 ggcaattcca ctaggattat taaaaaaaga aaaagagttt ggtgcttttt caggattagt
     1561 aggatttata gcaatgcata taggtacaaa tttttattta acaagagctg atttgttagt
     1621 accagcagaa cagatgtcaa ctaatggtca agctatgatt atgggtatac aaacatataa
     1681 tacaagtgtt ttaggtggta ttgtagcagg attaattgtt tatgcaatat atgataaagt
     1741 tagtaattta aagataccag aatcattagg attctacagt ggaccaagat tagttccaat
     1801 tgttagttta atagttatga gtatagttgg attattaata cctattgttt ggccaccatt
     1861 tttcaatgca ttccaaagtt taggaagatg gatttcttca gcaggaccat taggatattt
     1921 ttcatatgca gtggcagaaa gagttacaat tccatttgga ctaaatcatc ttgtaacatc
     1981 tgtattccgt tttacaccaa taggtggaac tgctgttatt gatggagaaa cgtactttgg
     2041 tacattgaac atgtttatgg catatgtaga aaataatcag attatacctc ttgatttagc
     2101 tggtaaaatg gaacaaggta aattgatgat tcaatatgga ttagctggtg ctgcattggc
     2161 tatctaccgt acagcaaaac cagaaaatag aaaggcactt aaaggacttt taatatcagg
     2221 agtgttgaca gttattatag gtggtataag tgaaccaata gagtttctat tcttattcat
     2281 atcaccagca ttatttgctt tccatacatt tatgaatggt atagcaaaca tggtacttcc
     2341 atacttaggc gtgttgatgg gattcacagg agatttaatt gcatttataa gttttggtgt
     2401 gttaagagga acagctacag gatggccaat agcagtatta gtagcagttt tatattttgc
     2461 aatatactat tttgtattta agtgggcaat tattaagttt aatataaaga ccccaggacg
     2521 tgaggataaa gaaataaaag tatctgaagg aaatggttca gatttcaaaa atttgtctac
     2581 atataaagga caacaaatga tatctgcttt aggtggtcaa tctaatatat tatcactaga
     2641 taattgtgta actagattgc gtttaaaact acaagatgta tcattaataa atgaggatgc
     2701 aattaaagag gctggtggta tagcagttgt taaattagat gagcatacaa tacaagtaat
     2761 tataggaact caagtgtatt ctttaagaaa gcagatggat aaagctatgg aaggtcattt
     2821 ggaggcatgt aatgattaat tttgataaaa aagtagaccg attgggtaca tattgtaccc
     2881 agtgggacta tgtagaagat agatttggta agaaggattt gcttcctttt acaatttcag
     2941 atatggattt agagtttcca tttgaaataa cagaggtgtt gcaggaaagg ttaaatcata
     3001 gagttttggg atacagtaga tggaaacatg atgattttag aaattcagtt acgaattggt
     3061 atattaaaag atttaattgt agcgttgata gtaactggat atattatagt ccaagtgtaa
     3121 tgtattcaat tagcaaactt attgagatgt taagtaatga aggagatgga atccttataa
     3181 atactcctgc atacaatgct ttttatgatg tgataataaa taacaatcgt aatattataa
     3241 agtcacatct tgtaaatagg aatggattct attcaattga ttttaatgat tttgagaaaa
     3301 agtgtaaaaa atctaagata tttattttat gtaatcctca caatccaact ggtagagtat
     3361 ggagtgagtt agaacttgtt aagatgatag gaatttgtaa gaataatgat gtcaagataa
     3421 tatcagatga tatacacatg gatattgtat atgacaatag aatgacacct atcttaaatg
     3481 ttgcaaagga ctatttagat tctgtattca tttgtacatc agcgtctaaa tctttcaata
     3541 taccagcatt gactgggtca tatgtgatta taccaaatga aggtattaga aataaatttg
     3601 aaaatatcac tagataccgt gattttgtaa attcgccagc aattctagca atattagcta
     3661 caatggtctg ttatgatgac tgcgaatatt ggttagatga actattaaaa taccttaaag
     3721 agaatcttaa atatacgatt gattatataa aagataattt agaaccattg aaattggtca
     3781 tgccagaagg ctgttacttt gcttggatag atttttccga acttaaaata agtggtgaga
     3841 aattccaaaa attattgatg gatattggag aagttgctat tatgtcgggg tctgtatatg
     3901 gtgaagaagg tgagttttat ttaaggttaa atgtggcatg ttcaagagaa aaacttgaag
     3961 atggtttaaa taggattaaa aaaactatgg attatattgc aaaaagaatt ttatagtagg
     4021 aagggagata gttatgggat atttaaaaac aatggttgat gttatgacta ctgaaatgaa
     4081 tgccataaga tgtttaatag atgaagcagg aatagaatat gaaagtatag ttagtgaaat
     4141 tgcaaattgt gaaggcaaag taatttttat gggtgtagga aaatctgcac atataggtaa
     4201 gaaattggca gctacatttg ctagtactgg tacacctagc ttttttgtac atgcaacaga
     4261 ggcagtgcat ggagatttag gaatgattga aagtaaggat attacaattt taatatcaaa
     4321 tagtggaaat tctatggaag tggtaaactg tataaaatat attaaagcaa taggttctaa
     4381 gacaattgca tttacttcaa acagaaattc agtactggca aaagagtgtg attatgcttt
     4441 aatatatcct gcaaaggatg aagcagacca tttaaatcta gcaccaacaa catcatctac
     4501 aattactttg gttttaggag attctattgc atgtgcatta tctaagagta gtaattttgg
     4561 ttcttcagat ttttataagt accatccagg aggaagttta ggtgagaagt taaaaacagc
     4621 taacaatgga taaaagcaaa aaggagagta gtttttatgt tcaaaatgtt taaaaaaaaa
     4681 tcaataaaat cacccatatc aggaaaagta atagagttat ctaaaataca agatggtgta
     4741 ttttctcaaa aaattatggg agagggtgta gctatagatt ctacaggaga cattgtatat
     4801 gctccaatca atggaaagat agtagtagta gcagagacta aacatgcatt tgtaatagaa
     4861 ttagaaaatg ggatggaact tcttattcat gttggtttag atactgtaaa tttaaaaggc
     4921 gaaggttttg aggtattggt gagtatggga gaacaagtga aggaaggaac accaatgttg
     4981 aaaattaacc gtcctttgat tgaatcaaag ggtatatcac ttattactcc agttacaata
     5041 actaatcatt cagagtacaa tatgaataca tgtaatgtag gcaatagtgt tgaaggtggg
     5101 aaagatactg taattgagtt taaataaaga actaaaatac aaggttaata tttataaata
     5161 cctataaact tatgttttat aagattgatt gtatagattt gttgtataga tttgttgtat
     5221 aaaaaagaac tctttaatta taaattaagg agttcttttt tattttgtaa atattgttta
     5281 gatttatata gaaaaacact tagtaataaa tagaataaat aagtatttat atagctaaaa
     5341 atctaaatag ttttgtttaa atatattttt atattttgta atttcaa
//
LOCUS       DAMKMI010000193         5355 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMKMI010000193 DAMKMI010000000
VERSION     DAMKMI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.199"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            209..1672
                     /locus_tag="PC429_003480"
     CDS             209..1672
                     /locus_tag="PC429_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF3848936.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            1728..2864
                     /locus_tag="PC429_003481"
     CDS             1728..2864
                     /locus_tag="PC429_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF3848937.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            3100..4215
                     /locus_tag="PC429_003482"
     CDS             3100..4215
                     /locus_tag="PC429_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF3848938.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            4367..5236
                     /locus_tag="PC429_003483"
     CDS             4367..5236
                     /locus_tag="PC429_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF3848939.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT         2064 a          543 c         1004 g         1744 t
ORIGIN      
        1 cgtcgttaaa aattttataa atttatgttt agatatgatt aatactcttg attttagaaa
       61 taaatatttg tagattgtag attaaagata atataaataa atagaaggta gattttttag
      121 atatattaat ataaagatta tataaaatat tgaaatatag ctgtttaaat tattgtctat
      181 aaatataaaa tgaatggtgg tgagttaagt gaccgattta gttaacacca ttaatggaat
      241 tgtttggtca cccgtattag ttgcaatgtg tttgtgtata ggattatatt tttcattgaa
      301 attaaaattt tttcaaataa gagatgtaaa agaaatgttt actctcttgc ttgaaggaga
      361 tagttctcat gttggtatat catcatttca aggttttgct acatctatgg ctggaagaat
      421 agggactgga aatatagctg gtgtagctgt agcaatagca atgggaggtc caggagcatt
      481 agtatggatg tgtataatgg ctacattagg ttcagcaact gcatttgtag aatctactct
      541 agctcaaata tataaagatg agcatgatgg gcagtataga ggaggacctc catattattt
      601 tgaaaaaggt cttgggtgga aaaagtgttc agttttattt tcaatagttg caataataag
      661 ttatacattt tttatgcctg gtacacaatc aaacacttta tatctagcaa tgaatcaagc
      721 atttggattt tcaaaaatga caatagcagt agcatcttct attgcattag cgataataat
      781 ttttggagga attaaaagga ttggtttatt tgctgaaaaa gtagtaccta taatgggtgg
      841 agcatacctt attatgacat tagtaataat tataacaaat attagtttag ttccagaggc
      901 tattgtaaca atgtttaaat ctgcatttgg atttggttca gtttttggag gaatgctagg
      961 ttcagcaata tcttggggag taaaaagagg tatatactca aatgaagcag gagaaggttc
     1021 tgggacttat ggtgcggcag cagcagaagt gagtcatcca gccaaacaag gattagttca
     1081 agcattttct gtttatgtag acacattatt aatatgtatg gctacaggta ttatgattgt
     1141 aataacagga atgtataatg ttacaggtgt ttgcacagca ctgccaggtg tagaaccggg
     1201 accacaatat gtacagagtg caataaatac attacatcca caattaggtg atatttttat
     1261 agcaatagtt ttattattat ttacatttac aactttatta gccttctcat atatgatgga
     1321 gactaatgta tcttacttag taactagaat taaagcagaa aaacatgaaa agtacataat
     1381 taatgtctgt agatttatgc ttatagcatg tgcagcaatg tgttgtttta tagaaccact
     1441 tacaagctgg gcattaggtg atatatgtat aggtgtatta acatatataa atttaattgc
     1501 catctttaca ctaaaaaaac cagctataaa ggcatattat gattatataa gacagaagaa
     1561 gatgggaata aaaaggagtg atagaacttt taaccctata gagttagata taagaaatgc
     1621 tgagttttgg gaaaatagat acttaaaaga ggataagcat gagttaaatt aaagttataa
     1681 taaaatataa aaattactaa ataaatttag tgggaggtaa gattggtatg aaagaaagaa
     1741 taatacaagt ggctgattca aagaaagaaa aaattctagg gctatgtcaa tctttatatg
     1801 atgaaccaga aattgctttg caagaatata aatctgctaa aaaaatatca gaatttttaa
     1861 gagaagaggg ttttgatgtt gaggaaaact tggcaggtat ggctacagct tttaaagcaa
     1921 ccaagaaaaa tggagatggt cctaaaatag catttatagc agaatatgat gctcttccag
     1981 gcaatggaca tgcatgtgga catcatttaa ttgcatctat gggagttgga gctggaatag
     2041 ctctttcaag tatactggat acttacaagg gagaagtaag tataattgga acacctgctg
     2101 aagaaactgg agatggaaag ccttacttga tagaacatgg tgtatttgat ggctatgatg
     2161 cagcaatgat gatacatcca aattcaaaaa cttgtgtaac acctgaaatt atagcaatag
     2221 gaggtctaga tttcatattt actggaaaag catctcatgc aggagctaag ccatataacg
     2281 gaataaatgc tttagatgca gtagtattac tatataataa tataaatgca ctaagacaac
     2341 aattagtaga tggaacgaga atacatggta ttatactaga agcaggaact gctgcaaatg
     2401 taatacctga tatgggaaaa gtaagattag agataagggc aaaagaacaa aattactttg
     2461 atgaagtagt agaaaaagtg aaaaattgtg ctagaggtgc tgcaatagcc actggatgtg
     2521 aacttgaatt ttatcacttt gaaccaactt gccaaggatt aaatgaaaat aaagttttgg
     2581 tagacatatt tacaaagatt atggaggaat ttggaatata tgaagatgaa caagtaatgt
     2641 taggttcaac agatatggga aatttaagtc aaataatgcc atgtatacat cctttgatga
     2701 agttttctga aaatggagaa gaactacata caaaagaatt tttggaggct tctataaatt
     2761 catatgcaaa agatagagta attgatggta taaaaatatt agctttaaca ggatttaatt
     2821 tatttgaaaa tccagagtta ttgaaaaaaa tgaaggaaga gtagaactta gactattttt
     2881 aattatactc tttaaaataa gaagagtgtc tcaaaatgaa ctttttagtt tatgagacac
     2941 tcttttttgt atgaaattaa atatattttt attattttaa caatgaaaaa gaggcttatc
     3001 tgtttttata tgaccttttt taacttgtag cacaaaatat catgaatgat tacaattcga
     3061 atgataaaat cgattataag atatatagga ggggtacata tggaaacttt agaagtaaga
     3121 aatattgaat tgagtggtac aaattatgag ataggataca gactaggaga attggttgct
     3181 aatatgccag agataataga aggtcagatt aataaatcta atctggtaag caaaaaagaa
     3241 gaaaaagaga tgatagagtt gtttgataaa tattgtccag gtcttaatga agaattgcaa
     3301 ggttttgctg atgcaattca agtgaattgt aatcaaattt tatattatac tatgacttat
     3361 cttaaaccag gttgtagtca agttgcatta gcaccagaat taactgaaaa tgggcatgta
     3421 ctatttgcac gtaatttcga tttctcacac aatatggagg attttgtttt atgtaaaact
     3481 aaggtaaatg gaaaatatgc acatataggt actaccgtta tgcaatttgg aagaggtgaa
     3541 ggtatgaatg aatgtggctt aggtgtaagt cagtcatcat gtggtattcc tgtaggcaat
     3601 tcagatggtt taagaaaacc agcaattgta ggtttacagt tttgggctgt tattagatat
     3661 ttgttagaaa attgcaaaga tgtagatgaa gcattagaat atttaaaaga tatgccaatt
     3721 gcatacaata ttaatttatt gcttgcagat aagtctggaa acattgcact tgtggagact
     3781 ttagatggga aaaaagaagt aaatataatc aatagtttag aaagtaaaag agaatatttt
     3841 ttacactcta ctaatcatac acatattgat aaacttcata aattagagcc acaatcaatg
     3901 aaaaattcta ttcatagata taaattaatt aaagaatata taaataagtc taaaaaaatt
     3961 ggagagaaag agttaatgaa tttattgtct tcaaaatatc caaatggctt atcttgtaat
     4021 tactataatg atttttttgg aactttaaaa agtatagtta tggatttaaa tataggaaag
     4081 tttaatattt tatggggtgg actagagaat aaatgggaga gctactattt aaaaaatgac
     4141 attaaatcta taacacagag gataaacata aatattgaaa aagcaccatc tgactttttt
     4201 gatttcattg aataaaaaat gtatttaatt attctaaata attaggatat ataaatcaac
     4261 taaaagtttt tatttatcgt attgaattag ttataatatt ttagtatagt aaaaaatagt
     4321 gctattttac atatttaaag agtttaagta gaagagaggt gccatattgg attacttaaa
     4381 gtattttaaa aaagctgtaa tgtatattga gaataattta catgaaaata taactgtaaa
     4441 tgatgtggca aaagaaactg gatattctta ttatcattta acacgcttat ttaagtctat
     4501 gtttggtgag agtgtaggaa gttatataaa aaaaagacga ttagtaagta gtacaaaaga
     4561 acttttatat agtgataaaa aagtgataga tatagctatt ggtagtggat ttgaatcttc
     4621 ggaagcattt agcagagcat ttaaatctat atacaaggtt agccctatag aatatagaaa
     4681 aaatagaatt gatgtatttg ttggtaaaaa gaaaaaatta gagttagatt ttatgagaca
     4741 tcttgttgga aatataacga ttaaaccagt tataaaagaa atagaaaaaa taaaggtaat
     4801 aggtataaag gataaagtaa ttttagaaaa taattcatta cctgacttgt gggagaaatt
     4861 cagaaaagta catcatattg tcccaaatac tttgccatca aagagagttt ttggaatctg
     4921 tgaagcaatc tctgaaatac acctagttag tgaaagtatg gaatttaatg agataatagg
     4981 acttgaagtt aatagttatg atgtgattcc aaattcattt gtttcaaaaa ctattaagga
     5041 aggaaagtat gctatattta ctcatactgg tagcttagat aatttagaca aaacttatga
     5101 atatatatgg ggtacttggt ttttaagttc aaaagaagaa cttgatataa gagatgattt
     5161 tgaagtatat gatgaacgat ttttaggtcc tagtaatgtg aactcacaaa ttgatattta
     5221 tatacctata aaataatcta taagaaaact aaaaaataat ttgattaaga aatagatgtt
     5281 cgtaaaagag catctatttt ttatttttat cagatttaga taattttaat aataaaagaa
     5341 ataaaaataa gttct
//
LOCUS       DAMKMI010000194         5337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMKMI010000194 DAMKMI010000000
VERSION     DAMKMI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.200"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            110..1600
                     /locus_tag="PC429_003484"
     CDS             110..1600
                     /locus_tag="PC429_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3848940.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1880..3376
                     /locus_tag="PC429_003485"
     CDS             1880..3376
                     /locus_tag="PC429_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF3848941.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3510..4424
                     /locus_tag="PC429_003486"
     CDS             3510..4424
                     /locus_tag="PC429_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3848942.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4542..4733
                     /locus_tag="PC429_003487"
     CDS             4542..4733
                     /locus_tag="PC429_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848943.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            4956..5306
                     /locus_tag="PC429_003488"
     CDS             4956..5306
                     /locus_tag="PC429_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848944.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
BASE COUNT         2119 a          530 c         1018 g         1670 t
ORIGIN      
        1 attcgataaa ataaaattta aacatataat attttatgta tatattaata aaaggacata
       61 ttgtgttata atggtatcgt ataaaatcat ttacgggggt ggtattaaaa tgtttcaatt
      121 tgaaacacaa atacacaaat taagacatga tgttttaact gcaatagcta gacttggaaa
      181 agaagataac cttaccagag aagcaatgga taatattcag tatgaaataa taaaaggtga
      241 gaaacctaca tacagatgct gtgtttataa agaaagagct ataatatctg agagagtaaa
      301 tgtaagtatg gggttacaac caggtcaaga taagatagat ttagatacaa tggatataga
      361 agaagatgaa caaattatat atattttgga agctgcatgt gacagttgtc caataaatga
      421 atttacagta acagatattt gtagaggatg tctagcacat agatgtaaag aagcttgtaa
      481 atttggagct ataacacatg tcggaggaat ggcctatata aatcatgaat tatgtaaggc
      541 ttgtggaatg tgtaaaaaag cttgtcaata tgatgccatc tcagaggttg ttagaccatg
      601 taagagtgtt tgtccaacta atgccttagg atttgataga gaaaatatga aagctatgat
      661 acatgaggat aaatgtttaa actgtggagc atgtatgtca gcatgtccat ttggagcaat
      721 atctgataag agtttgatag ctcctgtagc tagaaagtta gttcaaaaag agaaaatgta
      781 tgcggtggta gctccagcta taacaggtca agttgaagca aatgttacat atggtcaaat
      841 aaaaaatgct ataaaatcac taggatttgt tgatgtatta gaagcagctt gtggagcaga
      901 tgcagttaca gttcatgaaa gcttagaatt tgctgaaaga atggaaaagg gagaagcttt
      961 tatgacaaat tcttgttgtc caggatttgt tggttatata gaaaaaacat acccaaatca
     1021 tgtaggaaaa atatcttcta cagtgtctcc tatgatagct agtggtagat ttataaaaag
     1081 tcatgaacca gatgctaaag ttgtattcat aggaccatgt acagccaaaa agagtgaggc
     1141 agctaagaaa gaattaaaag gagctgttga ttatgtactt acttttgaag aaataatggc
     1201 attatttgag gcatttgata tagacttggc taagtgttca aatgaagata tagatgatgc
     1261 atcaattttt ggaagaggtt ttggagctgc tggtggtctt acaaaagcta tagaaaatta
     1321 tcttgctgaa aaaggtattg gtgttaattt tgaacctgta aagatttctg gttcaagaga
     1381 aatcaagaaa actatgacta tggctaattt aggaaagcta gaaggtaatt ttatagaagg
     1441 aatgatgtgt gaaggtggtt gtataaatgg ttctggtaaa ttcttatcag gtgcaaaggt
     1501 taaagccact tttgatagaa aaaattctca atcaactaag aaaagtgtct tagcaaatac
     1561 aaagataaaa gattttagta atataaattt agaaaaataa agataataca aagcaacaaa
     1621 aatacatatt gttaatagag ttgttctaag ttgaggttta acttttggac aactctattt
     1681 ttatttatat atttaattat tgaacaacta aaataaaaat tatatacact taaaataaaa
     1741 attacatata taatattttg tatataactt aacaaagtat atgcctatgt tataataatt
     1801 tatagaaata aataaaaaat acaatataca taatagtaga aatatagcaa aaaggtgtga
     1861 tttataggga ggattactta tgtttgaatt tgaaacgcaa ctaaaaaggt taaaacatga
     1921 agttttaaca gcagtagcaa gactatctat tgaagaaaat ttaacaaaag aaaatataga
     1981 aaaaattcca tatgaaataa ttaaaggaga aaaggcgaga tatagatgtt gtgtttataa
     2041 agagagagaa gtagttcttg aaagggcgca acttgcatta agtttaactc caaatagtaa
     2101 atatggagat ataaatccag attcagttag tttggacaca gatgagcaaa ttatatatat
     2161 agttgaagca gcatgtgata gatgtccaat aaatgaattt acagttacag agctttgtag
     2221 agggtgtttg gctcatagat gtaaggaagc atgtaaattt ggggcaattt cttatataaa
     2281 tgggagagca tatataaatc atgatttatg taaggcttgt ggaatgtgca aaagttcatg
     2341 tcaatatgat gctatatcag aagttgtaag accttgtaaa agcgtttgtc caacaggagc
     2401 tttggatttt aatagaaaca ctatgaaagc aatgatacat gaagataatt gtataaattg
     2461 tggagcatgt atgtcagcat gtccatttgg agcaatatct gataagagtt tgatagcacc
     2521 tgttgcaaaa aaacttgcaa agaaagaaaa tatgtatgca atagtagctc cagctataac
     2581 aggtcaaata gatagtaata ttacatatgg tcagttaaaa aatgctataa aatcactggg
     2641 atttgtagat atgatagaag cttcttgtgg agcagatgca gttacagttc atgaaagcaa
     2701 tgaacttatt gaaagaataa aatcaggaga taattacatg acaaattcat gttgtccggg
     2761 attttttaag tatatagaga agatgttccc agatgaaaaa gatagaatat catctacagt
     2821 gtctcctatg gtagcaacag gaagattagt aaaaaaaata gattctgatg ctaaagttgt
     2881 atttataggt ccatgtacag ctaaaaagag tgaggcaaca gagaaaagtt taaaagattc
     2941 agtagattat gtacttactt ttgaagaact aatggcatta tttgaagcat ttaatataga
     3001 tttatcaaaa tgttcaaatg aagatataaa tgatggttcg atttttggaa ggggatttgg
     3061 agcatctggt ggacttgcca aatcagtaca gaactatatt gctgaaaagg ggcttagttt
     3121 tgattttgaa cctataaaag tatctggtcc aagacaaatt aaaaagacaa tgacaatggc
     3181 taaattggga agattagaag gtaattttat agaaggaatg atgtgtgaag aaggttgtat
     3241 taatggagca ggtaagtttt tatcaggtgt aaaagctaaa aatacttttg atagaaaaaa
     3301 tggacaatct actaaaaaga gtgttttatc taatgataag attaaagaat ttgaagatgt
     3361 aaatttagaa agataattgt tttggtacag tcattttttt atcatgccac aaatatgcct
     3421 aaaatttgcc ctttttatat gtgtatttta cagagttaag gttgctatac tataaatatg
     3481 taataaataa aatttatagg agcttttgga tgaggaaaat agtattaaaa gatatcctgt
     3541 ttttagttac tacattatct ataatagtaa tattattgct tgtaacaaat ataactacta
     3601 atagtaagat aaaatcaacg aatgaatctt atttaaaaag tgacaaaaat gatgtgagtc
     3661 aaaataatat tcagagtagc acaaaaaata ctgcatttaa gactactata ttaagtactg
     3721 gaaagtctga ctgtatattg gttgaaatag gtaacaaagt cataatgatt gatacaggag
     3781 aagataaaaa tggaaaacaa atagtggata ggttaaaaga aaaaggaata aatacacttg
     3841 actatttaat tttaactcat ttagataagg accatatagg tggagtagat agtgtgttat
     3901 ctagtgttaa agtaaagaat attatacaag caaattataa gaaagatagt aagcaatatg
     3961 atgaatatat tgattcactt aaaaaagctg atatagaacc agtgctacta aaagataata
     4021 tgaatattgt aatcaatagt gcagaaataa acatacatcc ggcttcaaaa agcaagtacg
     4081 agagttcaaa tgattattca ataatcacta atataagcta tggagcacat aagtttttat
     4141 ttgcaggaga tgcagaggaa gaaagattgt cagagtttat aaatggaaat actttaaaat
     4201 atgattttgt taaaatgcct catcatggaa gatatgacaa attaactgaa acatttttag
     4261 agtctatttc accccaatat gctgttatta catgttcaga gaaaaaagaa ccagaggaag
     4321 gtgttttaaa aatattggaa agattgaata taaaaacatt tttgacttct aatggagagg
     4381 tagttatcaa tagtgatggt aagacattga gtgtgaatca ataatttttt taaaaatatc
     4441 taaaaaaatt taaaaaaatc attagcaaat attacccctt aaacatagta tattttagga
     4501 gctgaaactc cacatacaaa atgactagga ggtaacatat tatgttggat agattttttg
     4561 gtgaaggcgg atttggtgga tgtgcttggt ggataataat attattcttc ttattcttag
     4621 cattccaaga ttgttgggct gaaatggact tatgtgcatg gatacctttc ttaatattat
     4681 tattaatact ttgtgaatgt ggtgacgttt ttgatggagg atgtggatgc taaaaaaatc
     4741 aatttagatg atttaataaa gcttatggtg ggtataaata aaataccaaa ccaatcacaa
     4801 tctagtgttt gaaggtagtc ggaaatcccc cgctaccttc ttttttttat tgttaaaatt
     4861 tatttttgaa ttatttcatt tacatataat aatttttacg ataaagtctg atataattaa
     4921 aaagaaaata aaatttattt ataggagatg taaaaatggc tatagtggat aagattttca
     4981 atgagataaa acttatggtt gcaagacttt tattgggaaa aaaatacagt gaatatgaag
     5041 aaaaagattt agtgtataat acagaagaag aagttatact tataacttta aaaagattag
     5101 tttttcaggg aaatgtaaac gaagcagaag aaattttatt tgataaagct aagagtgtaa
     5161 atagtgaaaa tatgcagtat atagcaatag aattttatac tatgcttatg gaaaaaactg
     5221 atgaagaatt agaagctatg aatttttcta aacaagaagt ttatcaaggt atagaagata
     5281 ttagaaaagt attgaattta gaataaaaga tatatgaaaa tattgaaata aattaat
//
LOCUS       DAMKMI010000195         5319 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMKMI010000195 DAMKMI010000000
VERSION     DAMKMI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.201"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            305..1831
                     /locus_tag="PC429_003489"
     CDS             305..1831
                     /locus_tag="PC429_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3848945.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            1859..2416
                     /locus_tag="PC429_003490"
     CDS             1859..2416
                     /locus_tag="PC429_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848946.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            2938..3981
                     /locus_tag="PC429_003491"
     CDS             2938..3981
                     /locus_tag="PC429_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848947.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4092..5234
                     /locus_tag="PC429_003492"
     CDS             4092..5234
                     /locus_tag="PC429_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3848948.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         2253 a          442 c          938 g         1686 t
ORIGIN      
        1 aaaaatatta aactaactaa atcaataaaa aagttggtgg ataacaattt ataattgtta
       61 gcacaacatc atcttatgta ttgaaatcta aaatatcctc atttttactt agcataaata
      121 ctattaatta agaaaataat atagaaatta taaatatata taagtttagt ctggtgtata
      181 aaacaagaaa actaagtgat ttttaattta actttttatt aagcttattt tttatactat
      241 ttataataaa taaactccat gatgggaaaa ctattactat ttaatatgta aaggagtatt
      301 ttaaatgaat gaatcatatt ggtttttaaa ctcaagtcca aaagaatata acaaattagg
      361 tgaaaatata aaaacagatt gtcttatagt aggtggtgga ataactggat tgacaactgc
      421 atatttatta gcaaaagaag gtaaaaaagt agtgcttgtt gaggctgata aaataggata
      481 tggaacttct ggaaggaata ctggtaaggt aacttgtcag catgatatat tttactctaa
      541 gatagagaaa aaatatggat tagataaagc aaaatcatat tataatgcaa ataatgaagc
      601 tttaaattta gttgaacaaa taatagaaga aaacaatata aaatgtgatt ttaaaaggga
      661 aacttctttt gtatttacag aaaaagagga tactataaag gatattaaag atgaatatcg
      721 tacatgtaaa aaaataggaa taaattgtga gtatcatgaa actatagaaa atattccatt
      781 agatataaag ggggctatta gttttactaa tcaaggtcaa tttaacccaa agaagtatat
      841 tgatggattg gcaaaagcag cagtaaattt agggctaaaa atatatgaaa atacaccagt
      901 agtagacctt gaaaaaggaa aaatatgtag ggttaaaaca agagaggata atataataga
      961 agctgagaat gttataattt catctcatag tccatggtat gatggattga atttttattt
     1021 tgcaaaagaa tatgcagagc gtgcttattt aatggttgca gttttggaaa ataaattagc
     1081 agatggtatg tttataagta tagatgaccc atcaataact tttagacaat ataatgatgg
     1141 aagtgaaaat ttactgattt ttggaggagg agaccataaa gttggtcagg gaggaactga
     1201 gaaagaaata tttgatgatt tagagcatta tggtaaagaa gtctttaagg taaaagattt
     1261 taaggggaaa tggtctgctc aagacaatat gagttttgac aatgttcctt atattggtta
     1321 tatcaacaaa agagaagaca atatttatgt agctacaggg ttctctaaat ggggaattac
     1381 taatggaact gctgctggaa taataataaa agatttgata ataaataata attctgatta
     1441 taaagatact tttaatccat ccagattagg tagctacttt tcaaaagatt ttataaagga
     1501 aaatgcaaac gtagcaataa attatgtaag tggaaaatta aaaataggta gtggagatat
     1561 gcctaaaaat aatggagaag gaaagattgt taatattgat ggaaaaagat atggtgtata
     1621 taaggatgat aatggagatt tttatattgt agacactaca tgtactcatt taggatgtga
     1681 acttaatttc aacagtgaag aaaaaacatg ggactgtcca tgtcatggtt ctagatttga
     1741 ctataaggga aatatacttg aaggaccagc tcttaagcca ttaaaacttt atggacatgg
     1801 tgataatgat gttaatccta agttattata aaattatttt taggaaggta aagtgtatat
     1861 gaataaactt ttaaagccaa taactatact attgtatata gtttatattt tatctgccgt
     1921 attttttgta gcaaacaaag aatatggaag tctaggtctg gtagcaatta gtttagttgg
     1981 tactttcatt ttgagtttta taaataagca aacggaaaga ttattgagta aagaattata
     2041 tataatgctt gctttattta taatgttttc atcattgtta ggtacttgtt ttaattttta
     2101 tgatataaat tattatgatg attttttgca tttatggtca ggtataatat cttgtattgt
     2161 ggctttttct ataattaaat acttttatac acaaagagag ataagtaaaa tgagtaaagt
     2221 gtttttagtt atatttttat ttatgtttag tatgggagta gcgagtttat gggaaatagg
     2281 tgaattttta atggatactt ttgttgggac tactacacag gccggagggt tagaagatac
     2341 tatgattgat atggtagatg ctttaatagg cacaattata actattcctt ttatagtaaa
     2401 aaaatctaat atatagtttt aaatttttta tattaattat aatggattta attaatcatg
     2461 ttgagtttca aggtctatat gattaattaa atccattttt gttatttata attattattg
     2521 aatatatttt tacatataag atataaggtg atagatagaa attgttggat tatgtaaata
     2581 attaatatat aataccatta tgggcaataa atacaaatca aatgataata tagtctattt
     2641 atgtaaatgt tatattgtat actaagtata atagtaagtt ttttgatagt ggtgtagaaa
     2701 gtaaaaatct actagcctaa attgatgtat ggattagttt aggaacagta attgcatatc
     2761 ttaagtttga tgtcatgttg ggctactaga aataggtcat ctttttcact tgaatatctt
     2821 ttctttaaac atggagagta tcatatcaaa aacatatgaa aattaagtga tttataagaa
     2881 cagtaatatt ttatatgata gtatagaata gtaataaagt attaggagga acattgagtg
     2941 ttaaaaaagt ggtttggtat tgtgaaaaaa agacaaaaaa gtgagtcagc aaaagaagaa
     3001 ggtgaagtaa tattaaaaaa cgaaaaaata ttatctgaag aaaagttgat agatgaagaa
     3061 ggagttgtag ttaatattga taatgaaata ttaactaaag tagaagtagt aaatgatgat
     3121 aatgaaatag aagaaaaaat aatagaagaa gattggttaa ttagtgaaaa taccataaaa
     3181 ttagatgata aagaagcaat tattaatgat aaaaacatag aattatgtaa aaaagaagtt
     3241 caagttgaaa gtgaaaaaat aggtttaaat aagtttgaag gacttgacca aaatgaaaat
     3301 tataatctag aaaaaaatgt tattgaagaa aaaaaagtaa gtgaatgttt gacagaagaa
     3361 gatttagagt acataaaaga aattaaaata aaaagaggaa aaagtataaa agctataaac
     3421 ttgtatacta aagaagagtg ggtttttgac actcatatac agtgtagtaa aaaactcaaa
     3481 gttccattag ggtacataag agaaaattta aaatatggat atatggatta ctttggagat
     3541 gcaataaatt atttaagtga agtattaaat atagatgaat actgcaaaag tgaatggagc
     3601 tatctcgata atagcaaatc tccatctgag atatttaata ttctaaacaa taaaatattt
     3661 agtataaggc tttctaatga aaaaagaaat gaaattttga caaatgataa aattgaagca
     3721 ttaaaaatga attatagatt tgaatgtatt gatgaagaat atgatgaata ttttaaaaaa
     3781 tataagtcta taatcaaaag aggtggaaag aaaaaagttg aattagtaaa taaaaaaggt
     3841 gacattttag aaatatttaa gtctttagaa gaatgtgcca tttatttgca aaaggaaaag
     3901 aatgaagtta tacaaatgtt aaaatatgga gatacaaaag taggaagaaa ctttataagg
     3961 tatagtttga gaagtattta atttcatttt aaattcataa gagtttcatt tctgattcac
     4021 acatttaata ttattttaat gtaaaataat aaaggtgaag tattgcacaa ttaaaattaa
     4081 ggagggtatt aatgtctgaa gaaaaaaatc caaaagaaaa tacattagat gaatctaaaa
     4141 attcagataa tgtaaattct aaaaaagatg atgtgtcaga tgaaaaaaat actgataaca
     4201 aagttaaaaa aagggaaata aagctaaaca acaaaacact taaagctatt gtagcaattg
     4261 ttgcagtagg agctatatgt ttaggtggag gctatgtttt tggcaaagaa gctggaagaa
     4321 aattgcctgc tacttcaaaa cactataata gcagtgaggt agtagctact attggggata
     4381 aaaaaataac aggtgaaatg ttaagaaaga gaatggagcc attattttat ttaaatgcaa
     4441 aaactactat gactgatgac gaaatacgtt catatgaatc aaatatgatt gaaactatga
     4501 ttacatctga tatattatcc tctgaagcag caaaagagaa aataagtata actaatgacc
     4561 aactagatgc tcagtataat aatttaatga gtagtataga atcagcaatg ggaatgacta
     4621 aagaagagtt tttaaaacag tttgatttga ctgaaaaata tataaaagat gatatgaaaa
     4681 aagagctagt agcatcaaaa tatttaaatg aaaaatcaaa agttactgat aaagaagcag
     4741 agaactatta caataaaaac aagagcaatt atttacaagt tagagcttca catattttaa
     4801 taaaaactgt tgatgataaa ggtaagcaag ttagtagtag caaaaaagct gaacttaaga
     4861 aagaagctga agaaatactt aaaaaagccc aagctgggga agattttgct actctagcta
     4921 aaaaatactc tgaagatagt agtgcagaat caggtggaga tttaggtttc tttggtaaag
     4981 gtcagatggt ggaatctttt gaaaaagcag cctttgcatt gaagaaggga gaggtatcta
     5041 acaaacttgt agaaagtgat tatggatatc atataattaa gaaaactgat gaaaaatatc
     5101 agccatttga agaggtaaaa tcagatttag taagcagttt aacatcagaa aaacaaaacc
     5161 tacttataca aaatctaaaa gaaaaatata atgttaaaat tacagataaa tatcaaatga
     5221 aagctaaaga atagataaag tcaatttatt aaaaaaaaga aaaattaact gtatggataa
     5281 aagctatttt tactcttaat atagagagta taaaatagc
//
LOCUS       DAMKMI010000196         5304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMKMI010000196 DAMKMI010000000
VERSION     DAMKMI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.202"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..449
                     /locus_tag="PC429_003493"
     CDS             <1..449
                     /locus_tag="PC429_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF3848949.1"
                     /translation="KTELNTKIDQLIAGGSNVAYTQRVAIDDWVEDAESGFKATVTHS
                     LLTQRIVVNIIDATTKENIVPNFKIVDDNSIEIRSEVKVELNVYVINGNAETHFINAT
                     VDDNRVSEMTTYSSKKIEDRLVNIEEKVNGGLSNIATSVNELITYC"
     gene            466..2178
                     /locus_tag="PC429_003494"
     CDS             466..2178
                     /locus_tag="PC429_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848950.1"
                     /translation="MQTEWNFNYANYVQNVSLPPGRYKLECWGACGGAVDTSDWTDCA
                     KGGYSKGEIVFKKRTNLQICVGQSGYEKVSEGSSLTRSGFNGAGAAGKVTTGSFAYSK
                     YGGGATDIRLYHPSATWGNTESLLSRILVAGGGGGMKNNFASARSIGHGGGYVGVNGV
                     GRDRDFCGGGSQYQGGTSYDTEEYHGSLGKGGYGNIGIGGGGGWYGGAGSYSNECGGG
                     GSGYALNKDSYKVPGYIPTPEYYLENIVMTTGGNTTKADGYAKITLLQALPFLTVSSY
                     NSTQATFKADHTDPALLTKIEWFIDDVLKETITTNLTEEKTINYTLEDNALHTLKIVV
                     TDSNNATAEKVLSISKNIMPLPENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIE
                     ASLNNTLVELSEKIKTSFDSSDTSVQDLQNQVTQKNNTITQLETELSKRKRFITGTYT
                     FTKTDAENFNLSIYDKEGTSKTLTIPVNMGFSPSLIVLSGVTFSTTSKSYVYFDNVCN
                     SNFYNFGYNSDSTHSNPKAVGILNVSNVGYSSLVLTLYKLSMSEAVGIWAKEGATLTY
                     KIYI"
     gene            2199..2546
                     /locus_tag="PC429_003495"
     CDS             2199..2546
                     /locus_tag="PC429_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848951.1"
                     /translation="MNRNNRIIYDQTGNIWLQTGEATGDIQEWSKITELNFLDVEFGS
                     IDYSKQYIESINPVTKEPIIKDIEVILTDEQKRLQALEKELSMLKEENKNRDSEIVNT
                     AFEVENIKLNNNL"
     gene            2558..2728
                     /locus_tag="PC429_003496"
     CDS             2558..2728
                     /locus_tag="PC429_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848952.1"
                     /translation="MYNLLKLMIEQKNYSTKEDLQHKIDVFYAVNRITEEQYLELTSL
                     LNKEETPAEPIK"
     gene            2759..3325
                     /locus_tag="PC429_003497"
     CDS             2759..3325
                     /locus_tag="PC429_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848953.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTESKFTSENVEGALKECIDR
                     ADEAFQEADSGKTLLSTAIGSPATSEQTFQDYANYITGFKSNISNLETQLKSKYSIRY
                     GPIDGYDGNPFSANFGKSASYLIVYVYFRRSVYYYNPSGSSLGSNTGGSERAWITIDS
                     NKTGFSVHSYDTSYEPYPFTGYYIACFA"
     gene            3356..3646
                     /locus_tag="PC429_003498"
     CDS             3356..3646
                     /locus_tag="PC429_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848954.1"
                     /translation="MEEISINLLCAVAGVVISYLAFRNSSNRKIQDDTETTTKLEQQI
                     TFLCENVRDIKHDVAKFNTSFLDISERVAKVEASTKQAHLRIDEIINRIGGK"
     gene            3650..3907
                     /locus_tag="PC429_003499"
     CDS             3650..3907
                     /locus_tag="PC429_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF3848955.1"
                     /translation="MDNLISFIPEQLLILVAALYVIGAGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLNPVAVLQGVICWGVAIGVNQTYKQFKDGEK"
     gene            4168..5031
                     /locus_tag="PC429_003500"
                     /pseudo
     CDS             4168..5031
                     /locus_tag="PC429_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429958.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
     gene            5110..>5304
                     /locus_tag="PC429_003501"
     CDS             5110..>5304
                     /locus_tag="PC429_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3848956.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKAEEK"
BASE COUNT         2100 a          608 c          978 g         1618 t
ORIGIN      
        1 ctaaaacaga gttaaatact aaaatcgacc aattaatcgc aggtggctca aatgttgcat
       61 acactcaaag ggtcgcaatt gatgattggg ttgaggatgc agaaagtgga ttcaaagcaa
      121 ctgtaacaca tagtttatta acacagagaa tagttgtaaa tattatagat gctactacaa
      181 aagaaaatat agttccaaat tttaaaatag ttgatgataa ttcaattgag attagaagtg
      241 aagtaaaagt tgagttaaat gtctatgtga taaatggaaa tgcagaaact cattttataa
      301 atgcaactgt agatgataac agagtatctg aaatgactac ttattcatct aagaaaatag
      361 aggacagatt ggttaatata gaagaaaagg taaatggtgg tttatctaat attgcaacaa
      421 gtgtaaatga gttgataact tattgttaga gaggagagtg agaaaatgca gactgaatgg
      481 aattttaatt atgctaatta tgtacaaaat gtttcattgc cacctgggcg atataaatta
      541 gaatgttggg gtgcttgtgg tggtgctgtc gatacaagcg attggactga ttgtgcaaaa
      601 ggtggttatt caaaaggtga gattgtattt aaaaaaagaa ctaatctaca aatttgtgtc
      661 ggtcaatccg gttatgagaa agtttctgaa ggttcaagcc ttactagaag tggttttaac
      721 ggtgcaggcg ctgctggcaa agttactact ggtagctttg cttattctaa atacggtggt
      781 ggagcaactg atataagact ttatcatcct agtgcaactt ggggtaacac cgaaagtttg
      841 ctttcacgca tacttgttgc aggcggtgga ggaggtatga aaaataattt tgcttctgct
      901 cgttctattg gtcatggtgg tggttatgta ggtgttaatg gagttggtcg tgacagagat
      961 ttttgtggcg gtggttctca ataccaaggt ggaacaagtt acgacacaga agaataccat
     1021 ggttcattag gaaaaggagg ttatggtaac ataggaatag gtggtggagg gggttggtac
     1081 ggtggtgctg gttcttattc taatgaatgt ggaggtggtg gaagtggtta cgcactaaat
     1141 aaagatagtt ataaggtacc cggatatata ccaacacctg aatattatct tgaaaatata
     1201 gtcatgacta ctggaggtaa tactactaaa gcagatggtt atgctaaaat aacattacta
     1261 caagcattgc catttttaac agtatcttct tataattcta cacaggcaac atttaaagcc
     1321 gaccacacag accctgcatt acttacaaag atagaatggt ttatagatga tgtactaaaa
     1381 gaaactataa caacaaattt aacagaagag aaaacaatta attatacatt agaagataat
     1441 gcattacaca cacttaaaat agttgttaca gacagtaata atgctacagc agaaaaagtg
     1501 ttaagtataa gtaagaatat aatgccactg cccgaaaatg taaatttaaa tgatatatca
     1561 acaaaattag ttgaggttaa tgcaggattt aaagttggga aaacaagtat tataaacact
     1621 ttagcactta aaaatataga agcaagttta aataatacac ttgtggagtt atcagagaaa
     1681 ataaaaacaa gttttgatag ttcagacact agtgtgcaag atttacaaaa tcaagtaaca
     1741 caaaaaaaca atactataac tcaattagaa acagagttaa gtaaaagaaa aagatttata
     1801 acaggaactt atacattcac aaaaacggat gcagagaatt ttaacttaag tatatatgac
     1861 aaagaaggaa cttcaaaaac tcttactata ccagttaaca tgggcttctc tcctagttta
     1921 attgtactta gtggagtaac ttttagtaca acaagtaaat cttatgttta ttttgataat
     1981 gtttgtaatt caaattttta taattttggc tataattctg attctactca tagtaacccg
     2041 aaagctgttg gtattcttaa tgtatctaat gttgggtatt cttcattagt gcttacatta
     2101 tataaattat caatgtcaga agcagttgga atatgggcaa aagagggagc tacattaact
     2161 tacaaaatat atatttaata aaaaaatgag gtgataatat gaacagaaat aatagaataa
     2221 tttacgacca aacaggcaat atatggcttc aaactggtga agcaacagga gatatacaag
     2281 agtggtcaaa aataactgaa ttaaattttt tggatgttga atttggtagt atagactata
     2341 gtaaacaata tatagagtct ataaatccag ttacaaaaga accaatcatc aaagacatag
     2401 aagtcatttt aacagatgaa caaaagagat tacaagcatt agaaaaagaa ttaagcatgt
     2461 taaaagaaga aaataaaaat agagatagtg agatagtaaa cacagccttt gaggtagaaa
     2521 atataaaatt aaataacaat ttataggagg aattaacatg tataacttat taaaacttat
     2581 gatagaacaa aagaactata gcactaaaga ggatttgcaa cacaaaattg atgtattcta
     2641 tgcagtaaat agaattacag aagaacaata tttagaactt acaagtttat taaataaaga
     2701 agaaacacca gcagaaccaa taaaataggt tcttttttta ttgaaagaag gtgactaaat
     2761 gacttttaaa gagttagtta ataaagttag aaatcttgta ttagaagcaa agaatgtaac
     2821 tatagaagat acagaaagta aatttacaag tgaaaatgta gaaggagcat tgaaagaatg
     2881 tatagataga gcagatgagg cttttcaaga agccgatagt ggaaaaacac ttttatcaac
     2941 tgctatcggc tctcctgcta catcagaaca aacatttcaa gattatgcga actatattac
     3001 aggatttaag agcaatataa gcaatttaga aactcaattg aaaagcaaat attctattag
     3061 atatggtcct attgatggat atgatggtaa tcctttttct gctaattttg gcaagagtgc
     3121 aagttacctt attgtctatg tttactttag aagaagtgta tattattata atcccagtgg
     3181 tagttcttta ggaagtaata caggaggttc tgaacgtgca tggattacta tagacagcaa
     3241 taaaactggt ttttcagttc attcatatga tactagttat gagccatatc cttttacagg
     3301 ttattatatt gcttgtttcg cataataaat tatattatta aaactaggag gatgtatgga
     3361 agaaattagc ataaatctat tatgtgcagt tgcaggagtt gtaatatcct acttagcatt
     3421 tagaaatagc tcaaacagaa agatacaaga tgatacagaa acaactacaa aattagaaca
     3481 acaaataact tttctgtgtg agaatgtaag agatataaag catgatgtag caaagtttaa
     3541 tacaagtttc ttagatatca gtgaacgagt tgcaaaagta gaagcaagta caaaacaagc
     3601 acatcttaga attgatgaaa ttataaatag aattggagga aaataaaaga tggataattt
     3661 aataagtttt ataccagagc agttactaat tttagtagct gctctttatg ttataggagc
     3721 aggttgcaaa aaatataaac aattagataa taaatacatt ccagtagtgt tattgatact
     3781 tggaataggt ttctcaatat ggatgctagg attaaatcct gttgcagtct tacaaggtgt
     3841 aatttgttgg ggagttgcaa taggtgtaaa tcaaacttac aaacagttta aggatggtga
     3901 aaagtaatga aattaacaaa aatattatta ctcacaaaaa tttaaaaaat ttcattaaat
     3961 agagtatact tttatagcta ataagtatat aataatagta cagaactcgt aaagcattta
     4021 tatgctcaaa tatacgggac attgattttt gaaggaaccc gccaagcttc ttttatagct
     4081 caaatagtcg ggacagataa atttacccac taatattaat tggtgggttt tattatataa
     4141 aagtatatct atgaaaaggt gaaaacaatg gttgaagtaa aagaagaaaa aacatttgat
     4201 gaacaaatag atattttaaa aagtagagga ttaataataa atgataaaga agatgctaaa
     4261 tttgtattaa gtaatgtcaa ttattatagg tttacagcat atcttctaag ttttaagaat
     4321 gatgatggct catataaaga aggaactacg tttgaagaag tttatgatat atataggttt
     4381 aataaggaat ttaggatatt attaacagat ttgttaggga gcatagaaat agcatttagg
     4441 acatacattg catatacatt agcaattaaa catggtgctt gtggatatct ataaagggag
     4501 agtttcaaag atgaaaaatt ctatattaat tttttgacag cattagagag agaaaaaaat
     4561 aataattcag ataagctttt tattatacac cataaagaaa aatataaagg aaaacttccc
     4621 atttgggttg caacggaaat aatgactttt ggtatgttgt aaaaattata ttcaaacatg
     4681 ttgccagaag atactagata cataaaaaat aatttgtgta gagtgaatac tttattagtt
     4741 aaatcttggt tacaatcatt aacacaggtc agaaatcaat gcgctcatta tggtagaata
     4801 tataataata atttccgtat tataacaata aaaaacgaat ataaaaagta taacttggat
     4861 aataaaaaga tattttctta tatacttgct atgaagcatt tgactatgga taaattaatt
     4921 tggaatagtt tttttataaa acttcaaaag ttaattaatg attataataa ttctatagac
     4981 ttaaagctta ttggttttcc taataattgg atagagatat tggctaaata aaatagttac
     5041 tttaagaagt ttataaacac ttactatatg taagtgtttt tttattgaaa agaaggagga
     5101 aaataaataa tgaaaatatg tattacagta ggacacagta ttttaaaaag tggtgcatgc
     5161 acttctgctg atggagtagt taacgaatac caatacaata aatctcttgc accagtatta
     5221 gcagatacat ttagaaaaga aggtcataag gcagatgtaa taatatgccc tgaaaagcag
     5281 tttaaaacta aagcagaaga aaag
//
LOCUS       DAMKMI010000197         5301 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMKMI010000197 DAMKMI010000000
VERSION     DAMKMI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.203"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            171..1259
                     /locus_tag="PC429_003502"
     CDS             171..1259
                     /locus_tag="PC429_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF3848957.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            1287..2078
                     /locus_tag="PC429_003503"
     CDS             1287..2078
                     /locus_tag="PC429_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF3848958.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(2193..2747)
                     /locus_tag="PC429_003504"
     CDS             complement(2193..2747)
                     /locus_tag="PC429_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF3848959.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            2943..4145
                     /locus_tag="PC429_003505"
     CDS             2943..4145
                     /locus_tag="PC429_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF3848960.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            4202..5182
                     /locus_tag="PC429_003506"
     CDS             4202..5182
                     /locus_tag="PC429_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF3848961.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
BASE COUNT         2040 a          559 c          887 g         1815 t
ORIGIN      
        1 ttttctaagt gttatttatt tgtagagctg aataaaagtg aaaattctta tctagaattt
       61 attgatataa ttaaaaaaac aagtataaaa gaattaacta tagtatgttg tagctaaagt
      121 gttttaacag gataaaatat atcacgattt taagatagga gttgagtcat atgagagatt
      181 ttagcacgta ttctaaagat gatgttattt ttttattaaa agatatatca aatttaattg
      241 aagaagaagg aaataaagaa agagaattaa aaatacaaaa tggtagacat tattcagaaa
      301 tgataccaat agagtatgaa gttagtgaag attatctgaa tttatatcat gagaaactaa
      361 gagaaaataa agataaactt gcattttcta taggagttat gtgtgaaaaa ataattaaaa
      421 aaaatggaaa tgatgtagta ctagtttcac ttgctagagc aggaacacct attggtattt
      481 tggcaaaaag atatataaga agcaagtata atgtaagttt acctcactat acaatctcta
      541 taataagaga taagggaata gatatgaatg caattgaata tatagtaaag aatcatccga
      601 gctcaaaaat tcaattttta gatggatgga ctggtaaagg aactatatca aaagaacttg
      661 aaaaagcatg tgatgaactt aatgttaaat ttaaaaaata ttttgactca acacttgctg
      721 tattagctga ccctgctgga tattcaggac tttatggaat tagagaagat ttcttaatac
      781 caagtgcatg tttaaattca acagtttctg gtttagtaag tagaactgta ctaagagagg
      841 atttaattag taaaaatgac tttcatggag ctaagtttta tagacattta aaagataaag
      901 atgaatctat gaactatatc gaaactatag aagaatgttt taaaaatcaa tttaagaata
      961 tatctgatga agttgaaaac tgggaaagtg acattataac aagagatgga tactttgatg
     1021 ttttaaatat aaaagaaaag tataacataa cagatattaa ctttatcaaa ccaggtgtag
     1081 gggaaacgac tagggtttta cttagaagag ttccgtataa gattcttgtt aaagacttaa
     1141 atgataaatc tttagaccat atattcatat tagctaagga gaaaaatgtt gaagttgaac
     1201 aaatggattt aaaagcctat aaatgttgtg gaataataaa aaatatgaaa gatatttagg
     1261 aaatatatta gacaggagaa tattgaatga ttgttttttc agatttagac aggagtatca
     1321 tttactccaa taaattttta aatgctgata gtaaatatgc taatatagaa atttataggg
     1381 aaaaagaaat atcttatata tctttagata ctataaattt aataaagcaa atacaatatt
     1441 atggtatgtt tatacctaca actactagaa cagtggagca atttaagcgt atagaattta
     1501 ataaatatgg gatttatttt ccttggagta taacttcaaa tggaggagtt atacttaaag
     1561 ataatgaaat attgaaatca tggagtgaaa aaatagataa actaaaatct gattatgagc
     1621 ccattgaaag tatgattcat aaatttaaag actatttgaa tgtagatgga ataactaatt
     1681 ttaaagttgc agaagataca tttttttata tagttgttga tttatcaaga tttaatttgg
     1741 attcaatcaa agaatataca aatatattgg aatcaaaaaa ttggaagttt tatgtgagtg
     1801 gcagaaaaat atattttata ccaaaagaaa tttcaaaaga aaatgctata aaatacttga
     1861 ctaaagaatt aggaatagaa tatttttatg cagttggaga ttctattatg gactatggta
     1921 tgcttaatat ctcaaataaa tcctatgtat tgaagcatgg tgatataaat aaaaatgaaa
     1981 tagaaaattc atttatatca agttctttca atggaatgag tggaactgaa gagatactat
     2041 ctaacatttt agacgagaac tgcctactta atatataatg actgatatat aaataataaa
     2101 ctagaaaaat ctgaaataaa ttatagtaaa gtttatcata ctaatttatt tcagattttt
     2161 atcaatttat atttaagtaa gaactcaata tgttaaaatg aaggtggagt cattgcccag
     2221 actgagatac atacctcatc atcataattt tcatacacat gtggtatagt actgttaaaa
     2281 taaaaactat ctccttcctc caatagatat tccttatttc caagtgttat cttcatttta
     2341 ccttttatta tatagccaca ttcttctcct tcgtgtgtca caaattcact gtttttgtgt
     2401 gtatgaccat ctagtgtaac aagtataaat tctatttttt tcccagataa attaggaaca
     2461 agaagttcgt aaaattttgt agaatcatta gttactattt tttttcgatt atttttcttt
     2521 actactatat cagagtcttc tcttccaatc tcttcaaaaa aataacctat atttatatca
     2581 agagcttttg atattttcca cattgctaca atagatggaa caaccatatt acgctcaatt
     2641 tgacttatta agcctgtact taaatctgaa ttttttgcta aagtagagat acttatattt
     2701 ttagattttc taagagatct aattttgctt ccaacatcta tattcattag atacaaaccc
     2761 cctcagacat gattataaca tataatttat aaagattaat aaaataaaaa aaaagtttta
     2821 caatacaagt attttttatg ctatattgaa ttttctgaaa agatgcatta taatagtatt
     2881 aatattcaat atattgaata ttgataacga tattttaatt taatgaaatg gaggtaatta
     2941 agatgaagag ttttgtaagt cctgataata tttgggtatt atgggcagtt gtaactggat
     3001 gggcagcatt tagtatttat ttggaacaaa agtacaattg ggcatcaaaa gtgtcgggag
     3061 caataatagc tcttgtagga gcaatgttat tatcaaatct aaatattata ccagtagagt
     3121 ctgttgtata tgaccaagta tggggatatg ttgttccttt agccatagct ttattgttat
     3181 atcaatgtaa cattaaaaag atatggaaag aaagtggaag attgctcatt atatttcttg
     3241 taggttctgt aggaactgta cttggagcta tgattggatt tttagcatta aaaaatgtag
     3301 taccagattt aaatattgtt gcagctatga tgacaggttc ttatataggt ggaaatgtaa
     3361 actttgctgc tatgtcaggg gcgtttgatg cgccaggaga attagtatca gcaacagttg
     3421 tagcagataa tcttttaatg gcattatatt tctttgtctt aatagctata ccatcaattg
     3481 gattttttag aaaacatttt aaacatccac atgtagatga aatggaaagt ataggtataa
     3541 ctgaaggaga aacagtagca gcaaattatt ggggaagaaa agaaatatct ttaaaagata
     3601 tagcaatagc cattggtagc gcttttataa ttgtcgcagt atctgtagaa ctatcaactt
     3661 ggtttaaagc tataatacca ttatcaaatc cttttttagc aatgttaaat actttatttg
     3721 gaaatcaata tctaataata actactttga ctatgttgtt tgccacattt atgcctaatt
     3781 tctttggtga tataaaagga gcacaggaat tgggtacatt tttaatatat atattctttg
     3841 ttgtagtagg tgttcctgct tcaataacat taataattca aaaatcacca ttactacttt
     3901 tatactgcgg aataatggta ctaataaaca tgctagttac atttatagtt gcaaaaatat
     3961 ttaagtttag tttagaagaa gctattcttg catctaatgc aaatattggt ggtcctacaa
     4021 cagcagcagc tatggcaata tcaaaaggat ggtctaagct agtagggcca atacttatag
     4081 ttggaacatt tggatacata gtcggtaatt attttggtct tttagtagga aatattttga
     4141 tataatttta cgtgaaaaaa agttgttttt tagaaagatt tttatttaat tggaggttgt
     4201 tatgattttc gatggacatt cagatacttg ggcagatgta acaataagaa ggcttaacgg
     4261 ggaaaaagat gtatttagaa gaatccatct tgataatttt aaaagagcaa atatagaagg
     4321 agctattttt gtaatatgga tagacccacc atatgataaa gagcctgaaa aaagagttaa
     4381 acaaattata agctctataa aagatgaaat tgaagatatg aaagatgttg taaacatagt
     4441 aaaaaaatat tcagatatag ataaaggtat aagtgaaaac aaaataaata ttttaattgg
     4501 tcttgaagga ttaagccata ttggaagaaa tatagattta ctagattatt tttataaaga
     4561 agttggtgca agacatgcat cattaacttg gaatgaacag aatgaacttg ctacaggatg
     4621 tttaggggat gaaaatcgtg gtttaactga aataggtaaa aaagcagtca ataaaatgga
     4681 aaaattgggc atgatagtag atgtgtcaca tttaaatgaa aaatcatttt gggatttaat
     4741 gtctgttgca agtaagccag ttatagcttc tcattctaat gctaggagtt tatgtgatgc
     4801 taaaagaaat cttacagata atcaattaaa agaaatttca aatacaggtg gattagttgg
     4861 aataaatagt tttagaggat ttactaatag taatcctaag aagcaagatt taatacattt
     4921 ggttgaccat atagactata tggtaaatct tataggtata gaacatgtat cttttggttt
     4981 tgattttagt gattacttag ggaaagagac tataggttct tttactggaa atgctagtgt
     5041 gccaagtgta gatggtcttg aaagcacaca tacagcacag aacatagtag tagagcttga
     5101 aaatagaggt tataaaaaga aagatataga gttaatctct agtggtaatt tttataggct
     5161 ctttaaagat atactaaaat aatataataa agaacttaaa tttataaaat agtatattta
     5221 tacaaataat atctacatat ttaaattggc gttcataatt tattaaatta aattttaatg
     5281 ttactagaag tttcaattct a
//
LOCUS       DAMKMI010000198         5243 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMKMI010000198 DAMKMI010000000
VERSION     DAMKMI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.204"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            197..1108
                     /locus_tag="PC429_003507"
     CDS             197..1108
                     /locus_tag="PC429_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF3848962.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            1368..1802
                     /locus_tag="PC429_003508"
     CDS             1368..1802
                     /locus_tag="PC429_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848963.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            1825..3360
                     /locus_tag="PC429_003509"
     CDS             1825..3360
                     /locus_tag="PC429_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848964.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDAILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            3444..4811
                     /locus_tag="PC429_003510"
     CDS             3444..4811
                     /locus_tag="PC429_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3848965.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTAMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            4846..5070
                     /locus_tag="PC429_003511"
     CDS             4846..5070
                     /locus_tag="PC429_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF3848966.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
BASE COUNT         2163 a          446 c          989 g         1645 t
ORIGIN      
        1 tttataaatt taattatgaa ttagtcatca ataaacaaga aatttagctc tagaaatcca
       61 ttataaaggc tattataaat tttaatatgc taaaaatata aaaagaatag ttgacagtgt
      121 agaaaatatg gaataaacta aaagtgtaat agtaatatga aattatatat tacattgaaa
      181 ggtgtgaatg attattatga ataaaaaaat agaattaata gggaattttg caagtgattg
      241 gattaggtat agtgattatg aatataaaat tactgaagaa gatgaattat atatagttcc
      301 aacagaagat gctgtgttta ccatgtataa cccatttgat gtagcagatg atataatagt
      361 agatataatt cgcataggac aagaggcatt acaagatata tcaaaagaga caaaagaaat
      421 gaaactaaag aaagaagtac ttgaatttgt aaaaaagtat ggattattgg gtttaatata
      481 ttcaagtgta tataatagag atatagttgg agaagaaaga gtactgatga ttgaaaagaa
      541 ttatattaca aaagagaata tattgagtac agatgaatat gtaaacatgt ttataccatt
      601 tgcaagtgat gaagatgtgt attttaaaaa gtatagaagg ggtatagatg tatcaaagag
      661 agaagaatct ccaaagtttt ttggaaagag accattgatt ttagatttag tattttcaaa
      721 gttctacact gaacaactag attggattgt aagttttgct aaagatatgt gtaagcattt
      781 taatcaaatt tgtgtatata aaaatacatc taatctatta actgaaagtg ttactattat
      841 gtctggaggg tttaatcctc aaaagatagg attttctata agccaattag ataaaccaat
      901 tattgcatgg gagtttgatt ctttgaaaac agctattgag actatctatg cctttgcaat
      961 tacaagtgaa ccttcttctt taaataaatg caagtactgt ggtaaaattt atattcctac
     1021 aaattctaaa tctcaatact gttcaccttc ttgtagaaat tgctcaaatg taaagaaaag
     1081 tagaagtaga gccaaaaaag ataaataaaa ttaataaata tatcattgtt tagattttag
     1141 taaataacta ctgaattaaa agttgttata gaaagttata tatctaaatg atggtatttt
     1201 tttgttgaga aaattgaaaa gtttaattga gatagtaatt ttgagttgac aaaaaccact
     1261 tttaacacta ctattataag tggttggtaa aataatataa attaatttaa gtatagcata
     1321 aaaaacaaaa aaagtcaatg gaaaaaataa aaaaggagaa tttaaaaatg atatcaccta
     1381 ttattttgag ttctataaat cagaatttaa aggaaataga aagaaatgaa ctgcttgaaa
     1441 ctaatataga atctggagat tatggtctag ctctttctga atctgatgta aaagatataa
     1501 taaattcgag agataatact ttaaaggggt atggaagaat agaattagat ataaaagtta
     1561 caaaacagtt aatagaaaat atatatacat cacaatatac aaatgtggac aattatttag
     1621 aggctattaa tgatatgcaa gaaatttttt attatttaaa aaatgaaact gatgacaaga
     1681 tatgtgatga tgagattata gaaatactgg gtgaatttta tgaaaaattt tcaggcaata
     1741 tggacaatgt aagaggagaa gcagacgaat ttgcaaagaa atttaaattt ggagaagttt
     1801 aatatatgat aggagagatt ttgtatgtta aataatttaa gtaatattaa tataaacgaa
     1861 aataatttaa taaagaacca atattccata tctctactta aggaatgtct aaattgtaaa
     1921 gtgatagatg aaagagaagt atataatata caacaagaaa tatctctaat acttatggat
     1981 ttgattaaaa aatatacaaa tggtcaaagc acatcagtaa aaacagaagt tgccgaaaaa
     2041 ctgttgattt caatatggta tgctattgat gcatatataa ataagcttgg ggatatagaa
     2101 aaaagagttg tggagttgac tgctaagaat gttaaaaaag tgtaccttgg aggcattgaa
     2161 atattagagg aagagttagt taggctaaaa gatttttata aactaatgat gagtagaaaa
     2221 ttggatacta attcaatagc atataatgat accttgatgg agttatcaag tttctttaaa
     2281 agttatattg taaaatttga agctcaggac gttccagtaa gtatagatta tccattggca
     2341 ctagatgata tggatgttaa gggtatttat tatgtgaaga attatattga gagtatagat
     2401 atagaaaata gattttgtaa cctatttaag aaaaaagata gagaaaaatt attttatgac
     2461 tatggggtga cttataaaat agattgtgat gctatacttg taaatttatt tgaacttatt
     2521 ataaataatt gtattttttc tactatttta ggaaatgaag tgactaattt agaaatttca
     2581 aaatatgagt atgaattttt agaaaaacaa tttaaaaata tgtacaataa tattactgaa
     2641 gataaagtta tagactctga agaaagtaat ataattgaat tgattgaagt agatgatagt
     2701 tttaacactg aagtagatag taatttggat tataatgatt ttgaagaaat taacagagaa
     2761 attagaataa ataaaaaaat aactttacta cttatggaag caattgaagt attaataaaa
     2821 agattaagca tagaagatga agatgtaata agatatatta aaaaatatga aaagatattt
     2881 attgaatctg tgctaagctc aataaaaagt aacactttta aaagtatggt aactgtgagt
     2941 aaaaataaaa aggaacactt agaagattat attgttgatg atgaaagtaa attggatgat
     3001 gaatcgttta gagagatatt caatgagata ttagatagta catctataat tgaaaaaata
     3061 agaataataa aggaaaatat caatgctaaa aaagacttta tcgatattct tgaatctgaa
     3121 tgtttatttg gagaagagta tttaatgttg tttgcatcat taagtgaatt agaattggca
     3181 atattgggta gtgtagtatt ttatgaagat attagaatga gagaaattaa tatattagaa
     3241 tttatgttaa atgagaaaac tgaaactact ttatggaaga tagagtacat taaatttatg
     3301 aagaggcaaa gtgaagataa gatatactcc atagaagagc atatgaataa aattaattaa
     3361 caaaaaattg tgaataaata gaatgttagt atataatata atctgtaaag catatcaaaa
     3421 atacatatat ttggaggaaa attatgaaag agtatatcat aaataatgtt gaagaaataa
     3481 gagaagaatt aattgattta tctaagaaaa tatgggaaaa tccagaatta gcctttgaag
     3541 aaaagtatgc aagtagcatt caaaaagaat acctaaagtc aaaaggattt aaaattgaag
     3601 aagtagaaaa tttacctact ggatttatag caagttttgg agagggtaaa cctgtgattg
     3661 gcatacttgg agaatatgat gctttaccag agctatcaca atgtgtaagt gcagaaagaa
     3721 agccattggt agaaggaaaa gctggacatg gatgtgggca caacctgttg ggtgtagcag
     3781 gtgtaggagc agttgtttct ataaaaaaat taatagaaga aaataaattt aatggaacaa
     3841 taaaatattt tggttgtcct gctgaagaag aaggtggagg aaagacagct atgtgtataa
     3901 atggctgttt tgatgatgta gattgtgcat ttacatggca tccttttgat ataaatgcac
     3961 cttggagagg tggaagtctg gcaaacttat ctgtaaaatt taaatttaaa gggataacag
     4021 cccatgcagc tcaagctcct cataatggta gaagcgcact tgatgctgtt gaaattatga
     4081 atgttggagc taattatttg agagaacatg ttattgactc tataagaatg cactatgtta
     4141 taactaatgg tggtggaaga cctaatgtag ttcctggatt tgctgaaagt tggtacttta
     4201 ttagaggtaa aaaagcaaag gatgcagaac acgttcttga tagattaata aaagtagctc
     4261 aaggagcagc aatgatgaca gaaactgaaa tggagtataa ggttacagat ggaatatatg
     4321 actatatacc aaaccaatgt ttgactgatt tagtttatga caatatggtt tttgttggtt
     4381 gccctaaaaa tactcctgaa gaagaagaat ttgcaaaaaa gttgtgtgat actcttacta
     4441 gagaagaaag attaggtgta gcaacatcat tacaaaatga taaatctatt gtagaaagct
     4501 atatacacaa ggaaatagtt gacggtgaca aagataaagg actagctggt tctactgatg
     4561 ttggagatgt aagttatgta ataccagtgg ctcaatttgc aatggcagct tggcctgttg
     4621 gaattgcaag tcatacatgg cagtcatgtt catcagcagg ttcaaatata ggattttctg
     4681 ctatgataaa ttcagctaaa gtactggctt gtagtgctta tgatgtattt atggatacta
     4741 aaattattga tgaagcaaaa attgaatttg ataaatcatt agatggacaa aaatttaaac
     4801 cattggtata attttgctaa taaaatattt ctggggggaa tagctatgag agaaaaagtt
     4861 gaaaaagtgc ttgaggaaaa gataaaacca gtattacaaa gagatggtgg agatgtagag
     4921 cttatagatg taaatgaaaa tggtgtggtt ttagttagac tacaaggggc atgtagtggg
     4981 tgtccaggag cgactatgac aatcaaagct attattgaaa atgttttggt gagtgaagtt
     5041 ccaggagtaa ctcaagtatt aggtgtttaa tagttagcgt ttattaaatg tatgttatac
     5101 ataagtattt aataaagaag taaaaaaaat agtgaagcaa tatatatgaa aaagtatgag
     5161 aaagataata aaacattata gaaataatga aatatttcat atgtatatga agaaataaga
     5221 aaaaatagtg aagcaatata tat
//
LOCUS       DAMKMI010000199         5199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMKMI010000199 DAMKMI010000000
VERSION     DAMKMI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.205"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(596..2731)
                     /gene="rnr"
                     /locus_tag="PC429_003512"
     CDS             complement(596..2731)
                     /gene="rnr"
                     /locus_tag="PC429_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF3848967.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            3324..3938
                     /locus_tag="PC429_003513"
     CDS             3324..3938
                     /locus_tag="PC429_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF3848968.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            3956..4318
                     /locus_tag="PC429_003514"
     CDS             3956..4318
                     /locus_tag="PC429_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3848969.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            4424..4921
                     /locus_tag="PC429_003515"
     CDS             4424..4921
                     /locus_tag="PC429_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF3848970.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT         1692 a          795 c          632 g         2080 t
ORIGIN      
        1 ttttctatta aatgtttctc aattacttat ttttcaatta gccacttcta ttaagcattt
       61 cttaattact tatttttcaa ttagtcactt ctattaaaca tttcttaatt atttattttt
      121 caattagcca cttctattaa gcatttctta attacttatt tttcaattag ccacttctat
      181 taagcatttc tcaattactt atttttcaat tagccacttc tattaagcat ttcttaatta
      241 cttatttttc aattagccac ttctattaag catttcttaa ttacttattt ttcaattagc
      301 cacttctatt aaacatttct taattattta tttttcagtt ggctttttct attaaatgtt
      361 tcttaattac ttatctttca gttagctctt tctattaaac atttctcaat tacttatttt
      421 tcaattagct actttcatta aatatttctt aattatttat ctttcagtta actatttcca
      481 ttagatattt ctcaattacc tatttttatc atttatttta tagagtattg tgcactatat
      541 atttatacat atcataaata ctacaacttg ttatctctta ttttagctgt tttatctact
      601 cctctatttt ttcaactatc tcaaagtcaa tttctttaaa atctacatta acactttcaa
      661 cctttatctt aacaaggtca cctatcttat atgacttctt agttctttct cctaaaacac
      721 tataagaact ctcatcataa atatagtaat catcattcat atttgcaagt cttatcaacc
      781 cttctatggt attatctaac tcaacaaata ttccaaatga agttacgcta gatacaacac
      841 cctcaaattc ttgaccaact ttatccttca tataaagagc cttatagaaa tcatgtacat
      901 ctctttcagc taactctgcc tttctttctt tctcggatga ttgaacagat gcataatcca
      961 ctatagtagt aagctgttct tgtctttttc cacttatttt attatttaaa gattctttta
     1021 ttattctatg aatttgaagg tcaggatatc ttcttattgg tgaagtaaag tgacagtaga
     1081 atttagctgc taatccaaaa tgtccactac attctggtga gtacttagct tgttttaaag
     1141 aacgaagcat tatagtactg atagctcctt cttctttttt gccctttatt ttctctatta
     1201 taccttgtaa tgcttttgga tgtacttcct ctttatcacc ttttattata tatccaaatg
     1261 tagatataaa cttactcaat tcttccattt tttccaaaga tggtgtctca tgtattctat
     1321 acacaaacgg tattcccatc cagtaaaaat gttcggcaat tgtctcatta cttataagca
     1381 taaattcttc tattatctta ttagatattc ttctttcata tggctttata tctacaactt
     1441 cgccttttcc atttagaatt atctttgcct ctgggaaatt gaagtctata gctcctcttt
     1501 tttcccttct tgacataaga atcctagcta aaatttcagc atttttaaaa tcttcaacaa
     1561 catgttcaaa agtcttttta agtttttcat catccttttc taatatatca gatacttctg
     1621 tataagtcat tctagctttt gaatttatta cagattcaca tatttcatgt ttaaccacat
     1681 ttcctttatg gtctatttcc ataaatatag aaagagttag cttatcttca aatggattta
     1741 agctacatac tccatttgaa agagtttttg gcaacattgg aattacttta tctactaaat
     1801 aaactgatgt agctcttttt aaggcttcct tgtcaagttt atttttctct cttacataat
     1861 gtgtaacatc agctatgtga actcctagtt tgaagtttcc attaggtaat acttctattg
     1921 atatggcatc atctaagtct tttgcgtcat ctccatctat agtaaatatg tttaaatctc
     1981 ttaaatctct tcttcttttt atctcttctt gaggaatctc aacagctaca gcttctgctt
     2041 cttgcaatac tttctttgga aattcttctg gaagaccatg ttctcttatt atagaatcta
     2101 tttctacgcc tctttcacct ttttgaccta gtatctctat aattttgcct tctggttttc
     2161 tatcctcttg tggccatact gtaatttcac atactacctt atcattatct actgctccac
     2221 taaaatgttt tttaggtata tatatatctt tagtaaattt cttgttgtct ggtgttacaa
     2281 atccaaaagt tttacttgat tggaaggtac ctactattct agttatttct ctctttatta
     2341 cttttactat tcttccttct gctctctttt cttcagttgc aggtactact atttctgcca
     2401 taactctatc attatgcata gcaccattta tattgtcact tgagataaat aaatcctgtg
     2461 tatattcaac atcagattct acaaatccaa aacccttttt gtgtgataca aattttccaa
     2521 caaagtatcc catttgattg ggagatacta tttttccttt tttagtaaag cagatataac
     2581 catcttcttc taattcattt aaaaaattat aaaacattgg catttctgat gaatgtatat
     2641 caaatatttc tgctaattct tcttttttta gaggattata tgcactttca tttattaatc
     2701 ctagcaaact ttctttaagt cctggtatca tttttccctc ctgttttact tttcattata
     2761 ttattattta tattgtcttt tatatttctt cagacaaaac attttttact tatcacataa
     2821 ttttctatat tttcttatta ttataagtta aaattactta tttatatttt tgatttaatg
     2881 taatctttgt catactaact tcaaacaaat tatgatgttt tataaattat ttgtaaatat
     2941 cattataatt acttaagtta ttctttatca taattgctta aattactctt tatcacctaa
     3001 ttatacacca tcaaatagga ttggtaaaat attcatctca tataagtatt actatataat
     3061 aatttattct attagtatac tcaaatcatg taaaatttat ataactcaat taatttttta
     3121 aatacaactt tataatataa aacttataat aaaagtaaat ttatatgtta aatgaggttg
     3181 attttttatt attttttatt tatgctagta ctaaagactt gcaaacgttt tttgacaagt
     3241 ctttattttg tctaaattaa acaaactttt ttacaaataa atacataaaa tttaaactaa
     3301 tctatttatt ggaggagata aaattggaag aagtaattgc aaaaattatt actatagaaa
     3361 atggattcaa agaaattgaa aacattgcta aaaaaattgt aaaaaataat gactcaaaaa
     3421 agtgttatta tctatcaatc tacttatata ggtcagaata ttatcaagta agagaattgg
     3481 ctgtgtttat acttggtttt atatcagtaa ctatttctga agctttgtta tttttaaaag
     3541 ataatgtgag taaagatgaa aactggagag tacaagaaat actagctaaa tcatttgatt
     3601 attattgcag tgaagtagga tatgaaaagg cacttcctgt tataaaagaa tggcttaatt
     3661 ctgatagtcc aaacataaaa agggctgtga cagaaggtct tcgaatttgg actaatagag
     3721 actattttaa atctaatcct gacgttgcta tatcactact ttctagttta aaagaagatg
     3781 atagcgaata cttgagaaag tctgtaggta atgctcttag agatataagt aaaaaatatc
     3841 cagatttagt aaaaaaagaa ttagattcat gggatatatc aaataaaaaa gttgagtttg
     3901 tatataagct tgcttcaaaa tgtatcttgg caaagtaaaa tggaggtgaa cttatttgaa
     3961 acttcgtgat gagtatactt gcccattaga actaacacat gatatgataa aaggcaaatg
     4021 gaaatctatt atattatgga gattacgcat aggaccaact tccttatcaa aacttgaaaa
     4081 ggaaatcaat ggtattaatc aaaagatgtt acttgaacaa cttaaagaat taattgaatt
     4141 tggtcttgta gataaaaaga catatgaagg ttacccactt aaagtcgaat actcacttac
     4201 acctattcga gggaaacaaa tattggatgc tttggctata atgcaagata taggaattag
     4261 ctttatgttg gataatggta tggaagatat tcttaaggaa aaaggtgttt taaaataaat
     4321 agatacccac aaaaaagtaa gtaatttacc attattttca actggtatat aattaaatta
     4381 tagatatatt ctattaattt attataatag gaggtaaatt attatgaaag taactagtac
     4441 aggtatagta aatggtgtta tagaagataa atatggtaaa agaggaactc agttcaatga
     4501 aggtggtatg cctacatatt cactacctct aaagtttgaa gatgctccaa agaatacagt
     4561 atcctttgct gtatttttag aagataaaga ctctgttcct gtatgtgggt ttgcatggat
     4621 acattggtta gctgtaaata taacaaaaga tgagttaaat gaaaatgaaa gtgtaacagc
     4681 tactgatttt gtacaaggga ctactagttg gcatggtaaa ctaggaggtc ttgacagatt
     4741 agaggccagt gtatatggtg gaatggctcc tccagacaga cctcatgtct atgaaataca
     4801 tgtatttgca cttgatacta tccttgacct tgaaaaagga ttctatatga atgaattttt
     4861 caaagctatt gaagggcatg ttttagatac atttacacta aaaggaagtt attctaatta
     4921 attaggttga ctatagctaa tcattaatta aatgatacat ttaattgatg attagcttaa
     4981 ttgttattag aatattttct gcactttatc ttaatatata ttttagttga ctggtatttt
     5041 aatttctatt agattattat caatttatat aaattgatta aaaaatagct acgcacactc
     5101 gtgactttag tcatgagtta gtagctaaaa tagtcagcat atagggaaac ttatatgtag
     5161 agataggata gaacctatcc aatactactt gaattgcta
//
LOCUS       DAMKMI010000200         5126 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMKMI010000200 DAMKMI010000000
VERSION     DAMKMI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.206"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..195)
                     /locus_tag="PC429_003516"
     CDS             complement(<1..195)
                     /locus_tag="PC429_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3848971.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKADVIICPEKQFKTKNEEK"
     gene            complement(195..452)
                     /locus_tag="PC429_003517"
     CDS             complement(195..452)
                     /locus_tag="PC429_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF3848972.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            complement(472..702)
                     /locus_tag="PC429_003518"
     CDS             complement(472..702)
                     /locus_tag="PC429_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDF3848973.1"
                     /translation="MNEELFEADLKRHETRINKHGEEIDELKIANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQRGIF"
     gene            complement(734..871)
                     /locus_tag="PC429_003519"
     CDS             complement(734..871)
                     /locus_tag="PC429_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDF3848974.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITASEYKEIVGEDYI
                     A"
     gene            complement(872..1171)
                     /locus_tag="PC429_003520"
     CDS             complement(872..1171)
                     /locus_tag="PC429_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848975.1"
                     /translation="MIYLGNLMDTEEQNIKYVGMIHYEPNLLSEENLKQGILIEELPT
                     PKYTENKETKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            complement(1168..2399)
                     /locus_tag="PC429_003521"
                     /pseudo
     CDS             complement(1168..2399)
                     /locus_tag="PC429_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2434..2616)
                     /locus_tag="PC429_003522"
     CDS             complement(2434..2616)
                     /locus_tag="PC429_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848976.1"
                     /translation="MNINNVVVRILAERILSRGLNPLKNREFELDDVTNTDYRTAVEN
                     YIIEHSGVVEGIEPTA"
     gene            complement(2616..2909)
                     /locus_tag="PC429_003523"
     CDS             complement(2616..2909)
                     /locus_tag="PC429_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848977.1"
                     /translation="MNRANRIICDQTGKILLQTGEATGDILQHDTITELHCIDVEYGS
                     IDYTKNRIIGINIETKQPILEEIPVYLTEEEKRIQELENQILLNENEKVGGIL"
     gene            complement(2934..4628)
                     /locus_tag="PC429_003524"
     CDS             complement(2934..4628)
                     /locus_tag="PC429_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848978.1"
                     /translation="MQTEWNFDAVSSAQEIILKPGKYKFECWGARGGALGTPFESGFY
                     YGKGGYCSGEITLKKETTLYLYVGLDGRKGYNFNGAGYGNGASGGGATDIRLIGGTWD
                     NEQGLLSRIIVAGGGGGTYDKQHGGDGGGLKGTLGTSSTGAAAHGGTQFEGGRGRDKD
                     GSCDGFFGKGATPENPSSQSGGGGGWFGGAYPASGFGNGSGGGSGYVLTKDSYKPPGY
                     TPTSEYYFDNVVMTTGGNTTVVGNYSDGRAKITLLQALPFLTVSSYNSTQATFKADHT
                     DPALLTKIEWFIDDVLKETITTDLTLEKTINYTLEDNALHTLKIVVTDSNNATAEKVL
                     SISKNIMPLPEENVNLNDISTKLVEVNAGFKVGKTSIINTLALKNIEASLNNTLVELS
                     EKIKTSFDSGDASVQDLMNQLTQANNTISQLNAKYKVAGGWTTPVYTNGTEKALVYNS
                     TREAIRYDWITISNLGFIPNIFYAECDYVNSYTKSKNKLFVFACYDVPTSSSKDFVFT
                     CDIQLGSSDSEYKVTYCGLYQHNKLDIHMTDNLIHLPAFATINAVASYYWRAIKIY"
     gene            complement(4645..>5126)
                     /locus_tag="PC429_003525"
     CDS             complement(4645..>5126)
                     /locus_tag="PC429_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF3848979.1"
                     /translation="KTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTITIDDWVEDA
                     ENGFKATVTHSLLTQRIVVNIIDATTKENVVTNFKIIDDNSIEVRSETRSELNVYVIN
                     GNAETHFINATVDDNRVSEMTTYSSKKIEDRLVNLEEKVNGGLSSIATSVNELITYC"
BASE COUNT         1595 a          978 c          570 g         1983 t
ORIGIN      
        1 cttttcttca ttcttagttt taaactgctt ttctgggcat attattacat ctgccttatg
       61 cccttctttt ctaaatgtat ctgctaatac tggtgcaaga gatttgttgt attgatactc
      121 gttaactact ccatcagcag aagtacatgc tccacttttt aaaatactgt gtcctactgt
      181 tatacatatt ttcattattt attttcctcc ttcaactgtt tgtaagtttg gtttatacct
      241 attgatatac cccaacaaat cacgccttgt aagactgcaa caggacttag tcctaacatc
      301 caaatagaaa atcctatacc aagtattaat aacactactg gaatatattt gttatctagt
      361 tgcttatact tcttacagcc cttacctata atagagagag cagctactaa aattagcaac
      421 tgctctggta taaaacttat taaattatcc atctcttatc ctcctaatta attaaaatat
      481 tcctctttga actgcaaata taaagaaccc tacaagtgtt gtaatcattg taccaattag
      541 ccatttgagc atacttgtaa gtgagtttag attctcacac aatgctttta actctgcttt
      601 agactctata tttgctattt ttaattcgtc tatttcttct ccatgtttat ttattcttgt
      661 ttcatgtctt tttaaatctg cttcgaaaag ttcttcattc atgaaaacct cctaattttt
      721 gaattaaaaa agactatgct atatagtcct ctcctacaat ttctttatat tcacttgctg
      781 ttatcttatt cttttctact gccgttttaa cttgctcttt agtccaatta ccattattat
      841 agaaatctgt tattatcttg taccaattca tttatatcac cccatttgac attaattgaa
      901 atgttaaatc tgctattgtt tgttctgtag aatttacttt gtcttctatg ctacttttaa
      961 tatctgtata tctatagaaa acctctttag tatctatatt tataaataac tttgtttctt
     1021 tattttctgt gtattttggt gttggtaatt cctctatcaa aataccttgt tttaagtttt
     1081 cctccgataa caaatttggt tcatagtgta tcataccaac atattttata ttttgttctt
     1141 ctgtatccat taaatttcct aaataaatca taatgattct cctttctcat ccgaataaac
     1201 cttttttgac aatatatttc taaaaattcc actactaata aagaaaacta tatcatttat
     1261 tacaaacata ccagcacttg ggtatgtttc agtaagtttt cggtagctgt cgatatgttt
     1321 aagagtattt aaacttattt ttacaaaagg actactagaa agcgaatata tagcatatat
     1381 atatccatta tatatatcaa aattgtcata tttgctatcg tctgaataca caattaaatt
     1441 taaatttgcg tcatatttta ctaaagcact tttttgttgc tatcaatttc tatacctctt
     1501 tctgcatcag atacaataac aaaatcattt aaaaacttaa tgtttttcat atgcaaatat
     1561 cctccaattc taaaatcttt agcaatggta aaatcaaaat ttattttaga taagtaacaa
     1621 tctgtataac tacttgaact tgagtgttct gttgtagcat aaataccatt cttattgcaa
     1681 acaaatttac cttctttaaa attgtaaata ccattagaaa ctaaattttt agttaacatt
     1741 atatgcatat cagatattct aattttatga agtgtagaag aagtctcatc tccatatact
     1801 acataaataa attccccata agtacataac ttataataag caccttctat tgactgcact
     1861 tcaattcctg ttgccttatt tattttatat aatttagtat tgtcagatat aaataaatac
     1921 tcttgtgtaa cacagataca tgagaagtta gcattagcta aagtaatgtc aaaaactact
     1981 gtttcatcaa tagcattaat tttaattaga tgagtttctt taattacata aaaatatggt
     2041 tcttcatatt caaaagtttt gaagttccca ctacatcttt caatatattt tatagctcca
     2101 tttgtaacta aatacggata gttatttgaa agtgttgttt ctcttaaatc taatcgcccc
     2161 tcttttatat caattttatt ctttatttct tcccatgtat cgcttgtagt aacctctgca
     2221 cctttggagt ttaatgctgt tactacatta tttttagcat taactccact ttgaaaaacc
     2281 tcttttaatg ctccttctac attatcactt gtaaaattat tctctgtatc ttctatagtt
     2341 acattctttg cttctaatac aagatttcta actttattaa ctaactcttt aaaagtcatt
     2401 tagtcacctt ctttcaataa aaaaaagaac ctactacgct gttggttcta ttccttctac
     2461 tactccacta tgttctataa tgtaattttc aactgctgtc ctatagtctg tgttagttac
     2521 atcatctaat tcaaattctc gattttttaa aggatttaag cctctactta aaatcctctc
     2581 tgctaatatt cttactacaa cattatttat attcattata aaatacctcc tactttttca
     2641 ttttcattta gtaatatttg attttctaac tcttgtattc ttttttcttc ttctgttaga
     2701 tatactggta tttcttctaa aattggctgt ttagtttcta tatttatacc tataattcta
     2761 tttttagtat agtctatact tccatactca acatcaatac aatgtaattc agttattgta
     2821 tcatgttgca atatatctcc tgttgcttct cctgtttgca agagtatttt gcctgtttgg
     2881 tcacatataa ttctatttgc tctattcatt ttatttatca ccacctaatt tatttaataa
     2941 attttaatag cacgccagta ataactggca acagcattaa tagtagcaaa agcaggtaaa
     3001 tgaatgagat tatcagtcat atgaatatca agcttattat gctgataaag gccacaataa
     3061 gtgactttat attcactatc agaacttcct aattgtatat cgcaagtaaa aacaaaatct
     3121 ttgctagaag aagtaggcac atcataacat gcaaaaacaa aaagtttgtt tttactttta
     3181 gtatagctat taacataatc acattcagca taaaaaatat taggaatgaa acctaaattt
     3241 gaaatagtaa tccaatcata acgtatagcc tcacgagttg aattatatac aagagctttt
     3301 tcagtaccat ttgtataaac aggagtagtc caaccaccag caaccttata tttagcattt
     3361 aactgagata tagtattatt agcttgtgtt aactgattca tcaaatcctg tacactagca
     3421 tctccactat caaaacttgt ttttattttc tctgataact ccacaagtgt attatttaaa
     3481 cttgcttcta tattctttaa tgctaaagta tttataatac ttgttttccc aactttaaat
     3541 cctgcattaa cctcaactaa ttttgttgat atatcattta aatttacatt ttcttcgggc
     3601 agtggcatta tattcttact tatacttaac actttttctg ctgtagcatt attactgtct
     3661 gtaacaacta tcttaagtgt gtgtagagca ttatcttcta atgtatagtt aattgttttt
     3721 tcaagagtta aatcggttgt tatagtttct tttagtacat catctataaa ccactctatc
     3781 tttgtaagta atgcagggtc tgtgtggtcg gctttaaatg ttgcttgtgt agaattataa
     3841 gaagatactg ttaaaaatgg caatgcttgt agcaatgtta ttttagcacg accatctgag
     3901 taattaccta caacagtagt atttccacca gttgtcatga ctacattatc aaaataatat
     3961 tcagaagttg gtgtatatcc aggtggctta taactatctt tagttagtac ataaccactt
     4021 ccaccgccac ttccattacc aaatccacta gcaggataag caccaccaaa ccaaccaccg
     4081 ccaccaccag attgtgatga agggttttca ggagtagcac ctttaccaaa aaaaccatca
     4141 caagaaccat ctttgtctct acctcgccca ccctcaaatt gtgtaccacc atgagcagca
     4201 gcaccagtag aagaagttcc aagagtccct ttcaagccac caccatcacc accatgttgt
     4261 ttatcataag ttccaccgcc accacctgca acaattatac gagatagtaa accttgttca
     4321 ttatcccaag taccaccaat aagcctaata tcggtagcac caccaccact tgcaccatta
     4381 ccatatccag caccattgaa attataacct tttctgccat ctaaaccaac ataaagatat
     4441 aaagtagttt ctttttttaa tgttatttca ccactacaat aaccaccctt accataataa
     4501 aaaccacttt cgaaaggagt tcctaatgca ccacccctag caccccaaca ttcaaattta
     4561 tatttaccag gtttaagtat aatttcttgt gctgaactta cagcatcaaa attccattca
     4621 gtttgcattt tatcactctc ttttctaaca ataagttatc aactcattta cacttgttgc
     4681 aatactagat aaaccaccat ttactttttc ttctaaatta accaatctat cttcgatttt
     4741 cttagacgaa taagtagtca tttcagatac tctgttatca tctacagtcg catttataaa
     4801 atgagtttct gcatttccat ttatcacata aacgtttaat tctgaccttg tttcacttct
     4861 aacttctata gaattatcat ctataatttt aaagtttgta actacatttt cttttgtagt
     4921 agcatctata atatttacaa ctattctctg tgttaataaa ctatgtgtta cagttgcttt
     4981 gaatccattt tctgcatcct caacccaatc gtcaattgtt attgtttgag tagatgccac
     5041 attagagcca cctgcaataa gttggtcaat tttaatattt tgtttctcat tttctgtgtc
     5101 aattctagtg tttagctctg ttttag
//
LOCUS       DAMKMI010000201         5080 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMKMI010000201 DAMKMI010000000
VERSION     DAMKMI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.207"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            340..3174
                     /locus_tag="PC429_003526"
     CDS             340..3174
                     /locus_tag="PC429_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3848980.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            3217..4461
                     /locus_tag="PC429_003527"
     CDS             3217..4461
                     /locus_tag="PC429_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF3848981.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVVKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         1986 a          659 c          733 g         1702 t
ORIGIN      
        1 attattctta accttatcag ttttttcata tatcttcatc tataccgacc atatattaat
       61 tacattgacc atatattaat aaatgatatt acatagactt aagtatatta aaaaacttaa
      121 atttagttat ttaacaacat aatactctaa tttaatctat aaaaatccat acctaatata
      181 atgcaactct tattttaaca ccatctatta cacaaaacaa catattaaaa agtaaaatac
      241 aaaatatcta ctataaacct tattttaata ctgaatatat attataatat atttaatgta
      301 gcttaaatat agtttaaaca taaagaaagg aaacggtata tgctaagaaa agataaagtt
      361 ctattatctc tacataaatt atgtgaaaat atgactttaa aagaccttca atcagataaa
      421 acaggattca ctacaataga aattgctaac cattcatctc ttgatagaag taataccagt
      481 aaagaattaa ataacctata caatgaaaaa aaagttctga aaatatctgg taagccaatt
      541 ttatttctac atgctagtat tatagaaaat ttaattggcc gtactttgaa cagtgatgaa
      601 ttttctataa ataattgtaa tgagctactt aaatcaagta aaaatgactc agaagatatt
      661 ttttcaacat tacttggaca tgattctagt cttaaactac ctatagaaaa agctaaatct
      721 gctatactct atcctccaaa aggtcttaat acattactaa taggacctac tggtgttgga
      781 aaaagtacat ttgctgaaac aatgtacaaa tatgctgtag aaagtaaaat cttttcttct
      841 gaaagcaaat tcgtagtatt taactgtgct gaatatgcag aaaatgctaa cttattatta
      901 agtaatttat ttggttatgt caaaggttct tttactggtg ctaataagga taaattcggc
      961 atagttgcac aggctgatgg aggcatatta ttcttagatg aaatacacag attaccacca
     1021 gaaggtcaag agatgctttt tcttttgatg gacaaaggca tatacagacg attaggagaa
     1081 tctgacattg tgcataaagc taatgtcttt attgtgtgtg ccacaacaga agatatacat
     1141 tcttctctgc ttggaacttt tttaagaaga atccctgtta caatcaacct acctgcttta
     1201 caggataggt ctttaaaaga aagattatct cttattaaat acttctttat tatagaatca
     1261 aaatacacca atgcaactat aaaagttcac aaagatgtaa ttaaatcatt actcctttat
     1321 gattgttttg gtaatatagg tcagcttaaa tctgacatac aacttatgag tgcaagagcc
     1381 ttcttaaatt acaaaactgg acttaaggat aatatagaaa tagatttatc ccttgttcct
     1441 gattacatct atattggtct tttaaaattg aatgaaaatc gtagtaatct aaataaatta
     1501 tcagaacttg attatatgct tttttatgaa tttaatggta ataacttaga agttgaatat
     1561 acaaaagaac tatctaaata caatatagat acatcaaaca atggatttaa atactctaca
     1621 gagattcctt atctggaaaa tgatataagg agtatgttta taaattacat ggaaaagtac
     1681 tctacaaaat tactttcgat atcttcagta actcattctg attatacaag taatgagatt
     1741 tttaaagtaa tcaatccaaa gatttactat gctgttgaac attctttaaa agaggctgaa
     1801 aaaatatcga gaaacaaata cacaaaacaa acttatattg ctctttcaat gcatataagt
     1861 gcacttatag aaaatataaa tcaagacaca tataatccta agtacaattt atataatgat
     1921 aaaattttct tagataaaga tttaaatata gctaaaatag ttttaagtat aattgaaaat
     1981 gacttacaaa catctttacc taaagatgaa gtaaaattta tatccatgtt tctaaatagc
     2041 atgtatcttg aaccaaacaa tcaatctgaa agtataggag tcataatact tgctcatgga
     2101 aattcaacag caagcagtat gtgtgatgta gcaaattcat tgcttggtac aaaccattgt
     2161 aatgcaattg acatgcctct tgatgtaaaa gtaagtacca tacttgataa aactatagag
     2221 ttggtaaaag aatgtgacga aggtaagggt gtacttcttt taacagatat gggctctctt
     2281 atagcttttg gagaacttat ttcagaaaaa acaaaaatca aaactcgctc aattgaaatg
     2341 gtatctactc caatagtttt agaagcagta agaaaaagta cactaccaga aatgactttg
     2401 gatgaattag ctaattcctt aagctcttta aatccatata ttggacgagc agcaatcgat
     2461 tcaacagaaa taaactatcc tgaaaagtca agagagtatg taataataac tacttgtata
     2521 acaggatatg gttcagctat gaaagtagca gactttataa attcttcact taaaaatatt
     2581 agtaaataca atattaattt agttcctcat aatatagaaa gttttaaaat gtataagaat
     2641 aaatttagta atactaaaat tctggcagtt gttggaagtg tagattttaa tgtagctaac
     2701 acccctttta tacacataga ggaactacta aatggatatg gcctagatat gctaaacaaa
     2761 ataatagaag gtacattcaa tgccaatagt attacaaata taaaagaaac aaatctatca
     2821 tacaacagtt taataataaa ctttatgacc caaaacttag agcttttgga ctctgttaaa
     2881 ttattcaact atgtcaattc ttccttattt acaataatag aattaataga tgaaaactta
     2941 aagagcaaat ttaagctagg atatatacta cattgctctt ttatgataga aagatgtttt
     3001 aaaaatacaa cttttccata taataacatt actgaattaa ttagtaaaaa tcatgaacta
     3061 tatagtttaa tacgtgaagc ctttaagcca acagaagctt attttaatat tttgatacca
     3121 gacgaagaaa ttgcttatat aatagacatg attaatgagt atctaaataa ataaaattta
     3181 tagcacttat tttgtgcata ttaattaata tatgaaatgg aggacgagat tttgaaagga
     3241 aaaataaaac agttaacaat acttgcatta atttttatat ttataactcc tgtatttgca
     3301 tttgcagata ctccaccagt accaaattct tcaagagcag cactacttat agaccaggaa
     3361 actaaaagaa tattatttga aaaaaacatt gatgaaaaaa tgccattagc gagtttaagt
     3421 aaaatgatga cctttttatt ggcaatagaa gcagttgata aaaatcaagt taaagagact
     3481 gatatggtga aaatagacaa gtctacagct tcagtaggag gttctacttg taaacttaaa
     3541 gatggtgatg aaatatctct aggtgagctt atgcagggtc taatgctagt atctggaaat
     3601 gatgctgcta tagccatagc taaacatatt ggtaaaactg aaaagaattt tgtaaatatg
     3661 atgaacaaga aagcagaaga aataggaatg attgatacgt attattttaa tccaaatggg
     3721 cttcctatat atactgaccc agaacataaa gaacctccta ttgaaaatat gtcaactgct
     3781 cacgatatag ttacacttgg aaagtatatg tacgaccatt atgaaaatca agttacaaga
     3841 ataaccacta tgcaagtata taatgatact aaaaaggatt ttacacatta taacacaaat
     3901 cctctgcttg tttcagtacc tggtgttgat ggaataaaga caggatatac agataatgct
     3961 ggatattgcc ttgcattttc tatgatggta cctaaagatg ctaaaaacga aagaaatcat
     4021 agattgatag gtgttgtact tggtgatggt aacaagaaaa atagaatttc ttcttccgct
     4081 actttattga aatatggtaa agataatttc cattctaaaa agatagcaca taagggtgat
     4141 attatagaga ctccttgtgt tgatggtatt gatgatttta agataacagt taaggttgat
     4201 aaagatttgt atggtgttgt ttcagataac gaaaatatca atcctaaggt agtgtttaaa
     4261 aatatgaatt atcctatcca taagggagat attgttggtg ttgtaaagta ttataatgac
     4321 tctggtaaat ttgttggaag tgttgatgta aaaagtgaaa gtaatatagg atgtattcct
     4381 ttgaaggata agattaagat taaggttgct aagataaata aaaaacttga gattaaaaat
     4441 tctgtttgtt ttaaagcata gtaaagcata ttattaaaag ggtatgttaa atttataaag
     4501 actccacttt gtggagtctt tttgtttttt acaaaaaatt aaaatagtgg caacaattga
     4561 ggatgtgagg gagagttgcc actattttgt atagggtatt atattatgtt tttaagctag
     4621 ttttactatc ttctatgttt atataataac tcttgtgtgt gaatttaatt gataattaat
     4681 atgaaaatta tgtgaagttt taagttagtt gacttagact attgtaaatt ttttcagaaa
     4741 aattctgttc ataagtctat tagaccaccg aattatttac ctataatatt ccgatcaaaa
     4801 aatctattct tttttaaaag taaggtcatg attattgtac atatataaaa attccgaaaa
     4861 ttaatctaca cactagtaac attaagtata ataaatttat tttagtaaaa ctattaaaat
     4921 aacctcataa gaaactatag attaaatcaa tcaatctaat ctacagtttc ttgattattc
     4981 cttaatattt ataatttaca ttatttgttt attttaaaac tatctgttac aaatctagtc
     5041 aatgttgaaa ataaattcgc tctgaatata gtagatgatg
//
LOCUS       DAMKMI010000202         5042 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMKMI010000202 DAMKMI010000000
VERSION     DAMKMI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.208"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            24..1907
                     /locus_tag="PC429_003528"
     CDS             24..1907
                     /locus_tag="PC429_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF3848982.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2132..2812
                     /gene="pgmB"
                     /locus_tag="PC429_003529"
     CDS             2132..2812
                     /gene="pgmB"
                     /locus_tag="PC429_003529"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF3848983.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2809..>5042
                     /locus_tag="PC429_003530"
     CDS             2809..>5042
                     /locus_tag="PC429_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF3848984.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKI"
BASE COUNT         2081 a          455 c          958 g         1548 t
ORIGIN      
        1 ttataaagga gggaaatttt aaaatgtcta ataaaaataa agaaaatata actttaagta
       61 atcaaatcaa aggaacaatt caaaatagtt taacaaaaga agctatatta catataccaa
      121 tgagcaatta tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa
      181 aaaatgaagc gaaaagagta attttacgaa taggagacca atatgtgtgg gataaaggag
      241 gtgcaggagg aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatatag
      301 taatgagtaa agaagtagaa actgaattat ttgactattg gatagctaag tgtaagcctt
      361 taaataaacg ctcacgatat ggctttataa tagaaggtca agaggaaaaa cttttattta
      421 cagaaaaaag aataatagag ttgggaaatg aaaatgatga aaaggaatta tgtgaaatag
      481 gtaatttttt tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg
      541 tgaaagatac tgtttggtat cagatatttc cagatagatt tgcaaatggt gacccgtcta
      601 taaacccaga aggagtagat aaatggggag ctattccaac cagagataat tttacaggtg
      661 gagatttaca aggtgtgata gaacacttag attatttgag tgatttggga ataaacggaa
      721 tttacttttg ccctattaca attggaaaaa ccaatcatag atatgatacg gtagattata
      781 tggaggtaga ccctacatta ggagataaag agactttaaa aaaactaata gaagaagcgc
      841 ataagagaaa tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta
      901 aacagtggca agatgtagtt caaaataagg aaaattctcg atataaaaac tggttttata
      961 taaaagatat gagtaaaatt gatactccaa tagaacaaat agatgaaaaa aatatacctt
     1021 atgaaacctt tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa
     1081 taaaatattt attggatgta ggcaagtact ggatacagga atttgatata gatgcgtgga
     1141 gattagatgt ttcaaatgaa gtagaccatg ttttttggag aaagtttaga gaagaagtca
     1201 aaaaagtaaa accagacatt tatatattgg gagaaatttg gcatggtagt ttaccatggc
     1261 taatgggtga ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat
     1321 tcttttgtac agatgagata aacgccgaag aatttaagta tatgataaat gatgtaatgg
     1381 taagttatcc aatacaagtt agtgaagtta tttttaattt acttggaagt cacgatacta
     1441 caagaatatt gacttatgct aatggaaata ttgatagatt taaacttgca tatatgttta
     1501 tgtttgtaca aacaggatgt ccttgtatat actatggtga tgaaatcgga atggaaggag
     1561 aattaagttc aatatctgaa ggtcaaagaa aatgtatgga atggaatgaa gaaaaatgga
     1621 ataaggatat atttgatttt atgaagaaga ttattagact tcgtaaagaa aataaagaac
     1681 ttaggtctat atcaaatgaa tgggtgttag cagataagga aaatggaacg attatattga
     1741 aaaaagaatc tatttctata ataataaata attcatctaa aaatataatt ttgacgttac
     1801 cagattattt agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa
     1861 aagaagaact aatgttagag aaatacaagt tcattatttt aaaataatac aaaaaagtat
     1921 ttattttaga tttcatgtta aattattata cagtttaaat aagtctttat aaacgataaa
     1981 aaaatatggt ctacaagtga gtgaattgaa agtagatttt aaattaaaga ctgagttaga
     2041 gtagataatt ttataaataa tatcttaaat acaagataga aaataaattg attattagtc
     2101 taaaaatttc ttgtagaaag aggaaataag aatgattgaa gcatttatat ttgatttaga
     2161 tggtgtaatt acagatactg catattatca ctatatggct tggagaaaac tagcccataa
     2221 agttgggata gatatagaca caaaatttaa tgaatcttta aaaggcataa gtagaatgga
     2281 gtcattggac aggatattag aatttggaaa taagaaatat tcattttcag aagaagaaaa
     2341 agtgagaatg gctgaagaaa aaaataatta ctatgtatca ttgatagatg agataacttc
     2401 aaatgatatt ttgccaggta tagaaagctt gttaattgat gtaaaatcta ataatataaa
     2461 gataggtcta tcttctgcaa gtaagaatgc aataaatgta ttaaatcatt tgggtataag
     2521 tgacaaattt gattttatag ctgatgcagg aaaatgtaaa aacaataaac cacatccaga
     2581 gatatttctt atgtcagcga aagggcttaa tgtaaatcca caaaattgta tagggataga
     2641 ggatgcaagt gcaggtatag acgctataaa ctcagctaat atgttttctg ttggtgttgg
     2701 aaattatgaa aacttaaaaa aagcaaattt agttgtagat tcaacaaatc aattaaagtt
     2761 tgaatatata caagaaaagt ataatgagta tatagttagg agaattatat gaaatgttta
     2821 tttagtaatg tagatgagtg gaagattata caagataaga ttgaaatcaa agaaaataga
     2881 ttagcagagt ccataatgag tataggcaat ggatatatgg gtatgagagg aaactatgag
     2941 gaaaattata gtggagatac acacaagggg tcttatatag ctggagtatg gtttccagat
     3001 aaaactcgtg ttggttggtg gaaaaatggg tatccagaat actttggtaa aattcctaat
     3061 tcagtcaact atataggtgt aagaattttc atagatgatg tagaattaga tttagcaaaa
     3121 tgtaaagtag aagattttta tagagaatta gatatgaaaa atggtatttt aaatcgtagg
     3181 tttattgtaa atataaatga taaaaaattt gaagttaggg ttgctaggtt tttaagtata
     3241 tctgttaagg aacttgcagt aattaaatac agtataaaat cattaaattc taatactaaa
     3301 ataaaatttg taccatattt agattcaaat ataaaaaatg aagattcaaa ctatgaggaa
     3361 aagttttgga atggaatttc ttcaagttct ttagaatata aaggtagtat tctttctcga
     3421 acaaaggaga atgcatttaa tacaccagta ttcactgtgg gttcaatgat gtatattaag
     3481 acaaatggtg atgtagaagc tgtatctcat gagagtgatt ataattactg tgaaaattcg
     3541 ataacaatgg atgttttaaa aaatgaggtt gcaagtatag aaaaatatgt atctataact
     3601 tcaactagag attataagga aagtgagcta ttaagaaaat gtgaagacat tttaaataga
     3661 gaactatcta aaagttatga tgatatatta aatgaacata caaagttatg gaataaaaga
     3721 tgggaaagtg cagatgtaaa aataaatgga gataaaagta gccaacaagg tataagatat
     3781 aatttattcc aactattttc aacatattat ggagaagatt cgcgattaaa tataggtcca
     3841 aaaggattta ctggtgaaaa gtatggagga gctacttatt gggatacaga agcgttttgt
     3901 gttccagtat atctaggtgt agcagataag gaggtaacta gaaatctttt agaatatagg
     3961 tacaatcagt tagaacaggc aaaggataat gcaaaaaaac tagggcttaa aggtgcttta
     4021 tatccaatgg taacatttga tggagtagaa tgtcataatg aatgggagat aacatttgag
     4081 gaaatccacc gaaatggaag tatagtatat gcaatatata actatacaaa ttatacaggt
     4141 gactataaat atataaaaga aaaaggaata gatgtaataa tagaaatagc tagattttgg
     4201 gcaagtagag ttcatttgtc tacaaaaaaa gatttgtata tgatacatgg agtaacaggt
     4261 ccaaacgagt atgaaaacaa tatcaataat aattggtata ctaattatat agctaaatgg
     4321 tgtttagatt atgctgtaga aaatatctta cgattagaga aagagtgtaa tgaatgtatt
     4381 gacagaaata atgtgactag ggaagaagtt gagttttgga aaataatatc aaataaaatg
     4441 tatctgccgt atgatgagga actacaaata ataattcaaa atgataattt tttagataaa
     4501 gaatttgtag aagtaaagga tttaccaaaa gaaaattttc ctcttaatca aaattggtct
     4561 tgggataaga ttcttaggtc gtgttttata aaacaagcag atgtattaca gggaatttac
     4621 tattttggaa atagatttac taaagaagaa aaaaagagaa attttgactt ctatgaaaaa
     4681 tatactgtac atgaatcttc tttatcttct agtatttatt ctattattgc ctcagaaata
     4741 gataacctag aaaaggctta tgaattatat tcaagaacag ctaggcttga tttagataat
     4801 tacaataatg atacaaatga tggactgcac ataacttcaa tgtctggttc atggctttcc
     4861 attgtacatg gatttgcagg catgagaaca tggaatgaaa ctctatcttt tgaccctaag
     4921 ttgccaaaag agtggaaaaa ttattctttc aatataaatt atagagataa tattattaat
     4981 gtagatgtat gtagtgattt aattaaaata gaaaatttaa aaggtagacc aataaaaatt
     5041 at
//
LOCUS       DAMKMI010000203         5041 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMKMI010000203 DAMKMI010000000
VERSION     DAMKMI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.209"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            43..1815
                     /locus_tag="PC429_003531"
     CDS             43..1815
                     /locus_tag="PC429_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3848985.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1837..3258
                     /gene="ccpM"
                     /locus_tag="PC429_003532"
     CDS             1837..3258
                     /gene="ccpM"
                     /locus_tag="PC429_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HDF3848986.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3287..4357
                     /locus_tag="PC429_003533"
     CDS             3287..4357
                     /locus_tag="PC429_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HDF3848987.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4373..4624
                     /locus_tag="PC429_003534"
     CDS             4373..4624
                     /locus_tag="PC429_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HDF3848988.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4675..4878
                     /locus_tag="PC429_003535"
     CDS             4675..4878
                     /locus_tag="PC429_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3848989.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2047 a          452 c          814 g         1728 t
ORIGIN      
        1 taaaatattt tatttcaatt ttattaagta tggaggtgta ttgtggaaaa tggtaaattt
       61 caatttttta aaggtgtaag aaattgtttt aatatgattt atctacatga taaaagatat
      121 ttatttatct ctatgtttat ggcaattttt caggggattg ttccatctat ttctttgtta
      181 gtaagtcaaa atatcattaa taatatgcaa acattttcga aaagtgtaaa agaaatatta
      241 gttttgttaa ttatgtattt aatcataaat ataattcctt caatttttca aagtttttat
      301 ggatattata gaataaagtt taataaaaat tttgaaaaac atattgaaac aatgatgtta
      361 gagaaatctt taactttgag tttaaaagat tttgaggatg ataatactta tgacatgata
      421 aatagagctc aatctcaaaa aggatttaat attactatac tatctgaaaa ccttatttca
      481 ataataagag aatcagtatc tacactttct cttattgttg tattatcaca atttcaactt
      541 tggctaataa tagttgttat tgttattcct gtattgaaat cattatattc cattaaatta
      601 ggaaaggaac aatatgatgt tagtgtaaac agaacttgga aagaaagaaa ggcctggtac
      661 atagattatt tgttgtcaaa aggttatgct tacaaagaga ttatggtatt tagaattggt
      721 tctaaactta ttaatcatta taaaaaatta aaagaggata ttatatctca agatatgaat
      781 atagctaaaa agaattttat tgctacgcta ttatgtacag ttttagattc aattgtatct
      841 gcaggaatta taggatttat aataattcaa ggtattttaa agaagatttt aataggagat
      901 gcgactacat atatacaagc agttcaatct ataaaggata atgttgaatc aatattttcg
      961 ttatttagtt cagtaataca agatcttttt tatacagatt tactgtttaa atttctagat
     1021 tataactgta aagaagactc aattgaacat ataaaaatat ttgaaaaaat aggggatata
     1081 aataaaatag agttaataaa tatttcatat aaatacaatc attcatcaga gtattcttta
     1141 aaaaatatta atttaacttt aaataaaaat gatcccattg ctatactagg aaaaaatggt
     1201 tcaggtaaaa cgacactttt aaaaatatta ttaggttttt atgatgatta tgaaggtgac
     1261 atcttgataa atggaaaaaa tttaaaggat attgaaaaag atgaatacat tagaaaagta
     1321 agttgtatgt ttcaagatta tattaaatat gaggctacat taaaagaaaa tgtcgccatg
     1381 ggagatgtat ataagagtaa cttggaagaa aaagttaaaa gtaaacttaa agaaactaaa
     1441 ttgaaaccaa gtatttatga ggaagatgga ttagaaacta ttttaggaac ctggtttggt
     1501 aagaagcaac tttctatggg tgaatggcaa aaagtagcaa tatctagaac tcttatgaaa
     1561 gatgctgata tgtacatttt tgatgaacca gattcatctt tagattctga agcgggaaat
     1621 gaactccttt ctttatataa aaaaacattt gaaaataaga ttggaatatt tataacgcac
     1681 catatccaac atgcaaaaaa gataactaac aatattgtag ttcttgaaaa tggaaagttg
     1741 ttagaaagtg gaagacatga tgaacttatg gacaaaaagg gtgtttattt taatttattt
     1801 tgtaactgca aataaatatt aaagggaggt taattaatga atcaaaaacc atttatacac
     1861 acatttaaag ctggaaaatc atattttatt tatgatgtta atacggataa gattttaaag
     1921 gtaaatgctg ctgtttataa ttatctaaat aaaattgagc ataatttaga aaaagaaagt
     1981 gactggaaca tagaaataga agacgaaatt aataccttga ttaattatgg atttctaaaa
     2041 aataaaaggg taagtaaaac aatgcaccct gaaactaagt atataaaata tcatatagaa
     2101 aataatctat cctctattat tttacaagta acccaaaatt gtaatttacg ttgtgactat
     2161 tgcatttatt ctggttcata cagcaacaga gttcatacaa ataaaagaat gaatatagat
     2221 gtagcaaaaa gaagtattga ctatttgatt aagaattcta gtcaattaaa tgaagttagt
     2281 gttggctttt atggaggaga acccttactt gagtttgatt tgattaagca gatagtagaa
     2341 tatgtaaaag tagttggaga aggaaaggaa atatacttta atctaactac aaatgcaact
     2401 ttatttaatg aggaaataat agaattcttt gtaaaaaata atataacaac tatgattagt
     2461 ttagatggag gaaaagaaat tcatgataaa agtagaaaat ttgcaaatac aggagaagga
     2521 tctcatgatg ttgtattaaa aaatgtacgt tacataaaaa ataaatatcc agaatattat
     2581 aaaaagaata ttacatttaa tacagtgata aattcagaaa atgattacat ttctatagat
     2641 gattttatat ctaaaaatga gactttaaaa gattcaaact ttttagcttc cactcttaat
     2701 aataactatt ctgataaaga aatagtttca tcagatgaat atatattagg taagagttat
     2761 gatagattct tgactttact caacaaatta aaatttataa aagatgagag tatgtcacct
     2821 ttatcaagaa tagaaagagc cttattagat gattttagat atgagaaaaa tattgaaggt
     2881 agattagagt taccagatgt gtggcatcat ggtggaccat gtataccagg agcttttaga
     2941 atatttgtaa ctacagatgg agaatttttt ccatgtgaga gagtaagtga aagttctaaa
     3001 atgggtatga ttggaaattt agatagtgga gtagatatag aaaaggctga aaaactttta
     3061 aatataggaa aattatatga agagcaatgt tctgattgtt gggcctatag ttattgtgat
     3121 atatgtatag ctaagattga tgatactaaa tgtaatgaaa atgaaataaa accacatgat
     3181 tgtgacagaa ttagacattc tacagaaaat aatatgaaag attactgcgt tttgaaatct
     3241 ctaggatatc aatattaaat tttctaatga aaggaaagat gggattatgg aaaaagtact
     3301 tatatatcca tttaattata atttttttac tattttaaat cattgcaata aaatttcaga
     3361 tatagaaatt aattcactgg ttagtccttc tggctggggg ttatgcaata aggagtataa
     3421 tgttaataat attattacta aagttacaaa tgattttcaa agtgaactta aaaagaattc
     3481 tttggtatgg tttgttgatt caaaagaaaa tctagactta gaaaaattta taattcctaa
     3541 attaaaagta gctctaaaag aaggtaaaaa gatattatat acacgtgatg atcttaataa
     3601 aatatctagg tatatatctc attatgaatt caaaaagatt aatgaatgga atttagaaaa
     3661 atatgaaata agagatatag agggtatatc acagataaac acaccaataa tatttatttg
     3721 tggtctttat ggagggttag atacttttga tgtaaatata aggataggag aagaactaag
     3781 aaaaaggggg ttttcaatat tacaatttgg ccctaaaaaa gagagctatt tatttaaaat
     3841 gattcctata ccagagttta tgtttgatga ttcgataagt agtaagacaa agattattaa
     3901 ttttaaccat tttataaaag ataaagaggt agaggcaaag ccagatttaa taattataga
     3961 agttccagga gaattattcc ctttctctag aaaaatggtt ggtaattttg gaattagagc
     4021 ttttgaaatt tctaatgcag taataaatga ttgtggtata ttatgtattc catcaagtat
     4081 atatgataga gaatattgtg aagaacttaa atattttatt aaaggaagac atggtataaa
     4141 tatagactat tttagtatag agaaaaaagt tatagataca tatgagacag aggtgaagca
     4201 aaaattttca tatatgtcat tagatgaaaa cttttttgtt gaagatatag aagattatga
     4261 aaaaacattt tttatacatg ataataaaca attactagaa ttagtggaca atatagtgaa
     4321 tcaattaaaa gagtatgcta aagtaatatc tatttagaat tggaggttaa ttatgaatta
     4381 cactgaaaag gaattactac tagagaatat attttttgat gtgactggaa ttaattttaa
     4441 taaaaataac aatttaaaaa aagaattatt tttttcaccg tctttacata tacagccaag
     4501 agaattactt ttagtatttt gcgaggtaat agaaaacttt aatataaaaa ttccagagga
     4561 aaaacttttg aataggcaat ttacgagttt tgataatgtt ctaaaaataa tcaatatggt
     4621 ataggttaat aaaggtataa agtaagtgat cttatagaaa aggggaggat atgaatgagt
     4681 tttggaaaaa gaatagaaat gaataatata ggtgttgaga caagaggtgt ttgtactatt
     4741 atgtgtcctt atacttgtaa gatgatggca tcttcatctt gtaactgcaa gaataatgat
     4801 tataatgcaa gaggaggatc atataataca tattataata aattgataca tggccaaaga
     4861 actcctgatg gaatgtaaat atttcagtaa taagtatact attaaattga tttagcatat
     4921 ttgattaaat ttatctttct tactttattt ccttttatta taaaaatatg cagttgatta
     4981 taattaagag tgtgatttta taataactgc ataaatagta tctgttatat aaatcacctc
     5041 t
//
LOCUS       DAMKMI010000204         4947 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMKMI010000204 DAMKMI010000000
VERSION     DAMKMI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.210"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            473..1333
                     /locus_tag="PC429_003536"
     CDS             473..1333
                     /locus_tag="PC429_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3848990.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            1323..3194
                     /locus_tag="PC429_003537"
     CDS             1323..3194
                     /locus_tag="PC429_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3848991.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            3214..4668
                     /locus_tag="PC429_003538"
     CDS             3214..4668
                     /locus_tag="PC429_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF3848992.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
BASE COUNT         1904 a          520 c          901 g         1622 t
ORIGIN      
        1 aatttgaagc tttacctatt aatatatctt aatagtgatt aaataaaact aaacttaaga
       61 ctttatatgt taatatatct taagaattta tttgttaaca tacagttcat tatatttaaa
      121 agcattacta tgataattca taatagtaaa atagataatg gttcagacaa tattataaat
      181 attagtgacg gtaaaatagt atcaatttag tatataaacc ttgtcagata atcgcataac
      241 agagcttgta gttatagaat aaaaaaatat ctaggaaacc gttgacagat ttatatggat
      301 agtataacat atagaatatg aaattaaaca aaaaagaatt ttaattatgg cgacgtagtc
      361 ataattttga gtatgttact gattagcagg cagaactcaa gtatatatgt gaaaaataga
      421 gggatactct atacatatat gcttgagttc tttttattga aaggaggaat atatgaaaat
      481 aattcagatt ttaaataata atgtagcctt agtaaaaaga ggtaaaaatg aaattattgt
      541 atttgttaaa ggaattggct ttaaaaaaag agtgggacaa gtaataaatg aaaatgaaat
      601 agagagaaca tatatactag attcatacga tatgctagaa cactttagtt atctactaca
      661 aaactcagac tccaataata tagttcttat taatgaaata attgaatatg gtgagaaagc
      721 tttaaatata aaggcaagtg attatctaag cttaacctta ctagaccatt tagagttttt
      781 acttaaaaga tgtgaaaaga atcaatttat aaaaagtcct ttggtatgga atgttaaaag
      841 attttatcca aagcattttg aaattggaat gcatgcacta aaattaattg gtgatagtaa
      901 gggaattaat cttcctgatg atgaggcagt atcaatagca cttcattttg taaatatgga
      961 ggtaaataaa gagtctcatg atagtacaat tgtagagttg agaactttag cagatatagt
     1021 ttctataata aaatatcatt ttaatgtaga gctagatgaa acttcaacaa attatatgag
     1081 atttactact catctacagt actttatcca aagaattatg tcggatgaag cttcaataga
     1141 tgaagataat tctatagatt tatataaaca agtaagtcaa ctttatccaa agtcatttgg
     1201 aagtgttcaa aagataagaa aatatataaa atcacaattt aatgtcgata tatctataag
     1261 tgaagaaact tatttaatgt tgcatataaa tagggtgact caaagactgg aggcaaaatc
     1321 agatgaaata taaagattta aatgaaaata taataaaact tataggggga aaagaaaata
     1381 taaagtcggt agcgcattgt gttactcgtt taagatttac tttaaatgat aggagtatcg
     1441 ctcaaacaga agaaattaaa aaattaaaag gagtaattga tgtagtttct aatgatgttg
     1501 cttatcaaat tataataggt actcatgttg tagatgtata taacgagttt atgtctatga
     1561 taggtttaag ttcaagtgaa tcaagtataa aaagtgaaag aaaaaaggga attagaggta
     1621 tcaaaggtat atttaattcc atctttgtgg tagtctcaga gactatgact tcagtaattg
     1681 aagtactact tgcagctggt atacttgcaa gtatattagc acttttaaat atgtctggca
     1741 tacttcagag tgacagtcct acatatcaaa tattagatac attaagaagt gctacatttc
     1801 acttcttacc agtattaatt gcaatagcat cagcaaaaag acttaatgta aatccctata
     1861 tagcagtagt gttagcagtt acgttattat cttcatcaat agatggtgta gaaggattaa
     1921 gtttatttgg tataaattta caggctacaa cttatgcaaa cagttttatt ccaatacttt
     1981 taggagtatg ggcaatggga attataattg aaggtttgaa aaaaatatta cctaaagcat
     2041 tacactattt tttagttcca gtgttatctt taataataac tctatcagtt acattaactg
     2101 tatttggacc tattggtatg tggattggtg atggattaaa ctatgcatgt atgtttttaa
     2161 ttgatacatt gggtaattgg agtgttgttg cactgtatgc agctttacaa ccactcttaa
     2221 tagtttttgg tgcagcaaac tttactatgc ctatatcttt aaattctgtg acaacattgg
     2281 ggtatgaccc aatttttatg ggcgctgcaa caatatctga tattgcagtt tgtggtgcaa
     2341 tgtttggata tttcttaaaa actaggaata aagaacaaaa acaactattt ggtacagtaa
     2401 gttttagtgc acttatggga gttacagaac cttcagtgtt tggtgtgttt atgaaatata
     2461 gaagaccatt tgtagcagta atgataggtg gaggtatagg tggtttaata gcaggtttag
     2521 ctcaagttaa aacatatgga atggtttggg gattagctgc attaccaacg tatcttgttg
     2581 gaggagaggt atctaacttt acatttatga taataagtgt agtagtatca tttgtagcag
     2641 caacagcagc ttcctattta ctaggaatac cttcagatga aaaagaagaa gttaagggtg
     2701 ataatgaaaa agaattagaa ctaataatca acgagaacaa ttcaagaact attagtattg
     2761 gaaatgttgc aaaaggtcag gtaattgcac ttggggaagt taatgacaaa gcattttcct
     2821 ctggagcatt aggtaaaggg gttggagtta tacctagtga atcaaaatca actgttatat
     2881 ctcctgtaga tggaactata actatagtat ttccaactaa acatgcatat ggtatagtga
     2941 ctgatgaagg attagaaata ttaattcata ttgggataga tactgtaaac ttagatggaa
     3001 aattctttga aagtcttgta acacaaaatc aaagggttaa aaaaggagat gctttagcag
     3061 tatttgaatc tgataaaatt atagaggaag gctttgattc tactataata actgtagtta
     3121 caaatactag cgattattta gatgtaatct catcaaatga tgaatcagaa gaattattaa
     3181 ctgtaatagt ataaatttta ggagggaaaa ttcatgaata ttaaagatac atttttctgg
     3241 ggaggaagta ttgcagccca tcaatgtgaa ggttcatggg atagtgataa caaaggacct
     3301 gcaattatgg actttgtaac taagggctct tatgaaacgc caagagtaat tactgataaa
     3361 atagaagaaa aattagatta tccatctcat aatggaatag atttttataa tagatataaa
     3421 gaagatatag ctttatttaa agaaatggga tttagtgcat taagaatatc tatagattgg
     3481 tcaagaattt ttccaaatgg agatgatgaa aatcctaatg aattagggat taaatactat
     3541 gaaggtctga tagatacatt aatagaaaat aatatagaac ctattgtaac actatatcat
     3601 tttgaattac ctatgaattt agtacataaa tatggttcat ggaataacag aaaacttata
     3661 gatttgtatt taaagtacag tgagactgta ataagaagat ttgatgataa ggtaaagtac
     3721 tgggttacat ttaatgagat gaaccatatt gaccctcaaa cagagcactc ggatattttt
     3781 acttatatga tagcaggtat taagtatagt gaaatagaaa ataaggttca aacattggca
     3841 aatgtagggt ataatatgac tttggcgggt gtaaaggttg caaagttggc tagagaaatc
     3901 aatcccaaca ataaaattgg atgtgtattt ggactaaatc caatttatcc atacaattgt
     3961 aatcctagta atgtgttaaa tgcattttta gataatgacc gtgattatta tcaaattgat
     4021 gcaatgtgca atgggaagtt tccacaatat aaattaaaag aatatgaaaa aaataatata
     4081 actattgaag tgactgatga agacaaaaaa gcatttaaag aaggtaagtt agattttata
     4141 ggacttaatt attatttttc aagtgtatct actccaaaag aaaatttatc aggtgataaa
     4201 tctttatttg gtgggattca aaatccatat ttggaacaaa gtaagtgggg gtgggcaata
     4261 gaccctatag gtttaagatt taccttgaac tatatttaca gaaagtatca attacctatc
     4321 ttaattacag aaaatggttt aggggcatat gataaaatcg aagaagatgg aactataaat
     4381 gatgattata gaattgaata tttaagtaag catctatctc aaatgaagtc tgcaattgaa
     4441 gaagataatg ttgaatgttt tggatatttg atgtggggac caattgattt agtcagtgca
     4501 actacaggtc agatgagtaa gagatatgga tttatctatg ttgacttaga tgatgaaggc
     4561 aaaggaagtt ttaagagata taagaaaaaa tcatttgatt ggtacaaaga agtaataaaa
     4621 tctaatggag aagttttaga tttttctacg gatatagtag tagggtagga ataagataaa
     4681 tagaagttag gaactaatgt taattagtgt ctagtaaagc atttaaaata gagtataggg
     4741 tctttaaagt aaagtgtgta ttcttcttaa aaataatggc aattaaaatt actataaaaa
     4801 ggagaataca cattttttat ttttatactt aagtaaatat ttagacatat tcatagtata
     4861 atttactcat tatataaaaa tagtatgatt tactcactat ataactttat aaattttctg
     4921 gaaaaatgtc agaatatatt gacaaag
//
LOCUS       DAMKMI010000205         4944 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMKMI010000205 DAMKMI010000000
VERSION     DAMKMI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.211"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     misc_binding    268..499
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            607..2088
                     /locus_tag="PC429_003539"
     CDS             607..2088
                     /locus_tag="PC429_003539"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF3848993.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     gene            2098..2991
                     /locus_tag="PC429_003540"
     CDS             2098..2991
                     /locus_tag="PC429_003540"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF3848994.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            3115..4176
                     /locus_tag="PC429_003541"
     CDS             3115..4176
                     /locus_tag="PC429_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF3848995.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(4331..4843)
                     /locus_tag="PC429_003542"
     CDS             complement(4331..4843)
                     /locus_tag="PC429_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF3848996.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
BASE COUNT         1841 a          608 c          765 g         1730 t
ORIGIN      
        1 taaacttttt ttgtaatata gtgtagtata aaattgtaat attacagata tttaatagtt
       61 atcttttgat tactttcatt aaaaacagct tcatcactat gaaaatctag gtttttaaac
      121 ttttaaaaaa gttaaaaaaa tatttttcta atactcaatt tgattacaat ttttttacaa
      181 tttaaaaaat attttttcca aatttttata ttaatacttg actttgtctg ttttataaac
      241 tagaataatt aataatatta agaatattga aaggcattga ttaggaagag taaatctgaa
      301 tcgattacca gagaaggaaa ctactagctg aaatgtttcc ctaattagaa ggattgaaaa
      361 ctacctagga gcttctatcc gaagttatct taatgatttt agtaggaata gacgttttta
      421 ttacttgcgt taaagtatca agtggatttt ttaaaatcaa tttgggtgga accgcgggaa
      481 acagattctc gtcccttttt tagggatgag ggtctttttt tattacctaa attcagtttt
      541 taaaatattc tgtatctaat ttaattagct aattaaaaat ttaaaatata tggaggatta
      601 taaaaaatgt attattcaag tacgagaggc accgaagaaa aagtaactgc atcacaagca
      661 ataataaaag gaatatcaaa tgatggtggt ctttatgttc caagtgaatt tcccaatgta
      721 aaaaatgaac ttataaattt agtaaattta acgtattctc agatagcatt ttttgtactt
      781 agtaaatttt tatgtgattt tactgaagat gaaattaaaa attgtataga aaatgcatat
      841 gatgaaaagt ttgattgtag tagtatagct ccattaaata aagtaaatga tacttatttc
      901 ttagaactat atcacggtcc aactttagca tttaaagata tggcacttac tataatgcct
      961 catcttctta aaacatctat aaagaaagat aatttagaga aagatgttgt gattcttact
     1021 gctacatctg gagatacagg taaagctgca cttgagggat ttaaagatat agataaaata
     1081 aagataattg tattcttccc agaagatggt gtaagcccag tacaaaaact tcaaatgaaa
     1141 actcaaactg gaaaaaatac atatgtagtt ggtataaaag gaaattttga tgatgcacaa
     1201 tcaggtgtaa agaagatttt ttctgataaa gaatttaatc aaaaactatt ggataataat
     1261 tatatattat cttctgcaaa ttcaattaat ataggaagac ttcttccaca agttgcatac
     1321 tatttttatt catatatgaa attagttagc aatagtgata taaatttaaa tgataaaata
     1381 aattttgttg tcccaactgg taattttggt aatattctag ctggatatta tgccaaacaa
     1441 atgggtcttc caataaacaa attaatatgt gcatcaaatg ataataatgt tctttatgat
     1501 ttctttaaaa ctggtgtata tgataaaaat agaacattga aattaactac ttctccttct
     1561 atggatatat taatttcaag taatttagaa cgattactgt ttgaaatatc aaatagaaat
     1621 actgatattg taaataagct tttatcagac cttagtgata aaggagtcta caaaataagt
     1681 gatgatatgg aaaaaaattt agcctcattc tatggagaat attcaaatga agaagaagtt
     1741 aaaagcacga taaacaatgt gtttaaaaat tataattatt tgatagatac tcatactgct
     1801 gttgcataca attgttatga aaaatataaa aaagaaactt ctgataacac aaaaactgtt
     1861 attgtaagta ctgcaagtcc attcaagttt tctgaagatg ttctaaagtc tatagattgt
     1921 gattttaaag accttgatga ctttgctata attgatagat tatcaagtat ttctaaaata
     1981 gatattccaa aacctattaa aaatcttaaa aatgcagaaa tactacataa agatatttat
     2041 gaaaaagatg aattaaaact agctataaaa aaattcttaa aggtataggt gattattatg
     2101 ttagagatta tagttcctgc aacaagtgct aatataggtc caggatttga ttgtcttgga
     2161 attgcgttaa atatatacaa taaattttat gttgaagaaa ttgaaagtgg tcttgaaata
     2221 gaaggttgtg aagatgctta caaaaacgaa aacaacttag tttatacatc tatgaagtac
     2281 ttttttgata gagttaaacc agaaaaaata cctgctggta tcaaaataaa aatccaaagt
     2341 gaagtaccta tatgtagagg tcttggcagt agtgcctctt gtatagtagc tggagttata
     2401 gctgcaaatg ctctatcagg tgctaattta gataaaaatc aattactaaa tatagcttca
     2461 gaaatagaag gtcacccaga taatgttgct cctgctattt taggaaatat gattgtatct
     2521 gttactgata atgagaatat acattatgat attataaaaa ttcctgaaga acttaaattt
     2581 tgtgcaatga tacctaattt taagttatca acagaaaaag ctagaggtgt tttaccaaag
     2641 gaaataccat attctgatgg ggtattcaat gttagtagag ttgctttact aatatctgcc
     2701 ttactaaaca aaaactttga cctacttaaa gtagcttgtc aagacaaatt gcatcaagat
     2761 tatagaggaa ctctaattga gaactacaat gatattgttg aaaaatctga acaattaaat
     2821 agtattggag tatttttaag tggagctgga cctactataa tgtctttaat aaaagaaaat
     2881 gatgatagtt ttgtggataa tatgaaaaat tacctacaaa aactaaaatc tgattgggaa
     2941 ataaaagagt tatgttgcga ttctaatgga gctgttttaa atataatata attatcgtct
     3001 tctatacaaa atataactac tcttttatta taatttgtag tataataatt aggactaggt
     3061 aataatatta agtattataa tctagttctt taaacatgtt tacattgagg agggattatc
     3121 ataaaagagt taataattag agatttagtt gcaaaagttc ctataattca aggtggaatg
     3181 ggcgttggta tatctaggtc atcactagct ggtgcagttg ctaagcttgg tggtgttggt
     3241 gtaatttctg gagttcaaat tggatatgat gaggaagatt ttgaaactaa tactataaat
     3301 gcaaatttaa gagctataaa aaaacatatt tctaaggcca aagaaatctc aaatggtggt
     3361 attattggta ttaattttat ggtagctatg aaagaatatg aaacatacgt aaaggaagct
     3421 gttaaagctg gtgtagacct tataatatct ggagctggat tacctaataa gctaccatca
     3481 ttagtaaagg gaagtaatgt aaaaatagca cccatagttt caactgcaaa agctgctaat
     3541 gtaattttaa aaatgtggga tagaaaagag aaaactactg ctgacttaat agttgttgaa
     3601 ggtccaaaag ctggtggtca ccttggatac tctaacgagg aacttgataa catagattct
     3661 atagactatg ataaggaatt tgttgaaatt ttaaaggttg caaatactta tggagaaaaa
     3721 tttggtagaa acataccagt agttgctgct ggtggtataa cttctagtag tgatgttaaa
     3781 aaatatatag atatgggtgc aagtggtgta caagttggta caagatttgt tgctacttac
     3841 gaatgtgatg cccatgagaa ctttaaaatg gcatacataa atgctagtga agaagatgtt
     3901 catatagtta aaagtcctgt aggtcttcct ggacgtgcca taagaaacaa atttatagaa
     3961 gaagttaaaa taaatcgtcc agaaataaag aagtgctata attgtttgat tccttgtaat
     4021 ccaaaagaaa ctccttactg tatttctcaa gctctaatca atgcagttaa aggagatgtt
     4081 gaaaattcac ttttattttg tggaaatgat gcctataaaa ttgataaatt aagtactgtt
     4141 gaagatgtta tcaatgaatt gatttctgaa ttataattta aagaaaacta aattaaaatt
     4201 ttatcaagct aaggagttta aatacgttat ttattaatct ttataaacat atttatgtcc
     4261 ttagcttttt tgtgtcaatt tccttttatt gaaattttaa tttaattaga gatagtatta
     4321 acccttataa ctaatcttct tttacaagta cattggttat ttcagctata tataaagtat
     4381 gataatcttt ttgtggatac catcttccat ctataccttc agcaataaaa cattggggtt
     4441 caagtttctg actatacatt tttttgcata taatagccat tttagcttcc ttaaaataag
     4501 gtgtatcatt taagtgttct atattaaatc caatctgtga tatcttatcc tcttctctac
     4561 cagatacttt tccacaatat gaaagctctt tacgaaaatc ttcatcaaaa aatgtaagag
     4621 aaaatttatc tgtattatct ataaattcct tagtataacg ctgtggtctt attactacaa
     4681 aagcaacatc ttttccccac ataactccaa gccctcccca gctagcagtc attgtattca
     4741 ctttaccttc attttctgct gttataagtg tccaatcttt acctatcaat ccaaatggat
     4801 tatttttaat ctcttctgcc aaaatttctt taaaatcact catatctcta ataacctctc
     4861 attctcttat atttatattc cttttatttt catgataaat tataaatgaa gtctagtttt
     4921 gaaaataaaa tactttttta aata
//
LOCUS       DAMKMI010000206         4866 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMKMI010000206 DAMKMI010000000
VERSION     DAMKMI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.212"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(482..1123)
                     /locus_tag="PC429_003543"
     CDS             complement(482..1123)
                     /locus_tag="PC429_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF3848997.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1366..2169)
                     /locus_tag="PC429_003544"
     CDS             complement(1366..2169)
                     /locus_tag="PC429_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3848998.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2325..3500)
                     /gene="thiI"
                     /locus_tag="PC429_003545"
     CDS             complement(2325..3500)
                     /gene="thiI"
                     /locus_tag="PC429_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF3848999.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3546..4697)
                     /locus_tag="PC429_003546"
     CDS             complement(3546..4697)
                     /locus_tag="PC429_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF3849000.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRLSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1514 a          928 c          464 g         1960 t
ORIGIN      
        1 gtttttatgt taggatattt cttagaaatg aaatacttct taatttgatt tgatatgtat
       61 atttttatag ctattttatt ataaaaaaaa agaacccttt tataactaag ggttactttt
      121 tttctctatt ttttcttatt atatcactat tttttcccta tttcaatatt ttgacatttt
      181 ttccattata ctgaaaagat atcttaaatt tttaacttgg ataaatttta aagcatatac
      241 ttaaaattaa catacttgtt ttctatttta ttacacttaa tccttatttt tatcattcta
      301 caaaacaaaa ataaaaaaga ataagataat aaataaaata cttatcacct tattctttat
      361 ttaattattt tacaacgaaa aatctaaact actttttaaa tagcttcctc ttccatcaat
      421 ttatttgtat attactggtt taattcttca agtaactgat tggttcttgc aatactttat
      481 cctaaactct ggaaacttta taaactacct ctctagcatt tggtaaattt ccatctggaa
      541 tacctacagc aataccaaat ggtacagaat acccttctgg tatatcaagt agttctctgt
      601 attttttatt tgtatcaaac ttcatcttta ttgactctga ttcttttaca tcttctttat
      661 ccaatacact cacaagacct tcaatatccc cttgttcaac aggtgtaaat aaacctctaa
      721 ccattcctat tatacaactt ccaagcccta gtgcttgtgc agctgttgtt atattccctg
      781 taagtattcc tatatcatat ggagcccatg aactactttc atcataagaa ataaataaaa
      841 cggttggagc accataaaat aacttaaagt ttttatttat ctcaacatta ctcacctcat
      901 gaatcctaga taaagtatca tcactaatct cttttaaaag tttctggtct tgcacaacag
      961 ttatgtgcca aggctgttta ttacatcctg aaggtgctgc aaatgcacag tgtaataatg
     1021 tgtttacttg ctcatcacta atttgttctg tcgtaaattt tcgtatactt ctacgacttt
     1081 gtattaaatt tattgtttgg ttgtcttgaa aattattatt catatctatt ccccttttta
     1141 tgtatttctt agtacaaata ctaaatcttc agaaaattaa tcttataatt aaatttcttt
     1201 acaatattat tataattatt tttagcttgt atgtgaagac ttaaaaatat aaacactttt
     1261 aatatatctc aattatatat ataaatgaaa atctggtatt cgtcatctat agaaactgct
     1321 cttttctata gtttaatcaa taccagatta ataaaacatg atatattaat ttaaaggacc
     1381 ccatccctct ttagaatgaa tctctgtttt tacagactta acactatctg tgtcaacatc
     1441 ccatgaccta cctgcaacag aaataccatt tttaccttta taccatctac cttcatcata
     1501 atcgtaaact agggaagtat accaattacc cccagtctta ttatttgcaa atacaactac
     1561 agtagcatca gatttagtag gactatatgc atatggttgt atatatagtg tatccttact
     1621 taatccatat tttgccttaa attcatctgg aattgtaggc cacccatttt tatatttatt
     1681 gtctaataaa tattttctaa aatcatcatt accctttgag gctcctggta ttatagaagg
     1741 gtcttgtgaa aatgatgcaa tcaaatcttt catactttga tgtatatccc tagccatttc
     1801 aacaccatct ggatgttttg tacaagtaac atacactttt gcaatgcttt ctattccagt
     1861 tattccatca taaccatcat caaacgttgc tgaatatatt ccacctgact tacactttgg
     1921 ttctgtttca aagtacttac catctttgtt ttctaatacc tctttcatga cttcattctt
     1981 gcctttactt gattcctcag ccattgcaat aacaatttga gtctttatat tttctctatt
     2041 agaaagacat gtaactgctt tacttttttc tatatttcta aacaatgctg gaacagctac
     2101 tataactaaa atgcctatta tagatataac tactaacaat tcaattagtg taaaaccctt
     2161 tttattcata ataatcattc ccattaaata aaattatagt acttattttt aaatactaca
     2221 tattaataaa ttgtctacat aaatcctttc aacagaacta ctaatatact tacagaacta
     2281 ctaatatact caatattcaa tttagtaatt aaataacttg tatgctaaaa ctcaatatcc
     2341 tctacctcta ttttgtcaat agcatcctgt actaattttt caatgtcaag tgctgtttca
     2401 gatttttcta ttttctctaa tctaggttta gtgacgggct ttctaggaga aaatacacta
     2461 caacaatctt cttcaggcaa tatagatgtt tcaaaagtcc ctattttttt ggctatatca
     2521 actatatctg atttatccat tgctataagt ggtctaaaca ctggtaagtc tacaacggca
     2581 ttagtacaag taagtccttg tatagtttga gatgcaacct gcccaatact ttcacctgta
     2641 attaaagcat cacaatgtct tttttcagaa agtctctgtg ctatcctcat cataaatctt
     2701 cttgaaagaa tagtagcttc ttcttcatta caattctcgc caattgcttt ttgtatttct
     2761 aagatattaa ctttatgaag ccttactcta ccacagtatt ttgccagtat tttagctaaa
     2821 tccttaactt tttcctgtga cctttcactt gtaaatggat aactatgaaa gtgaactgcc
     2881 tctacttcca tacctctttt tgcaaccatc catgtagcta ctgggctatc tatacctcct
     2941 gataaaagag acatagctct accattcgtt ccaagtggca atccaccata tccaggtata
     3001 gtatcactat agaccatagt atgaaattct ctgtattcgc actttatttt aacttctgga
     3061 tttcttacat caacattaat cctatctcct acctttgata ataagtatcc acctatatcc
     3121 atactcattt cttgagaagt caacccgaaa gacttatctc ctcttcttga ctctacctta
     3181 aaagttttat atccagcctc tattttttct tctaacattt ttaaagctat ttcttttaat
     3241 gtatcataat cttttttagc tcttactcca gggcacacac ccactatccc aaatactttt
     3301 ctaacttctt caattacttc ttcataatca tagtcttcta aatcaacata tattcttcca
     3361 tactctttat atacattaaa tcttccaatt ggttttaaca tatttcttat gtttcttata
     3421 agcctattct caaatatata tctattcttt ccttttactc caatttctcc atactttact
     3481 attaatatat tatacaccat ttcacctctt aaactaattt attatcacta tctatacagt
     3541 attctttatt ttcttttcat tattgacctt aagtcacaaa ttgattcttt caaaactttc
     3601 acagcttcta acatttcttc ttctgtattc atatcagata aactaaatct tattgttcca
     3661 tttatttctt catttgtaag acctatagca tttaatacgt aactaccttt ctttttagaa
     3721 gaacatgcag agccagttga tacataaata cccttttgct ctaagtaatg aagtaaaact
     3781 tctccttttg tccctctaaa tgatacattt aatatatgac atactccatc ttctggagaa
     3841 ttaattttta tatcttctat attatcaatt atttcatttt ttaatatatt ttttaagtta
     3901 tttactttcg aaataacaac atctaaatct ttgtttagta ttctaacagc ttctccaaga
     3961 ccatatattc caggtacatt ttctgttccc gacctaattc caatttcctg accaccacct
     4021 gttaacatag gcttaaggcg attattttct tttatataca taaatccaat tccctttgga
     4081 ccatgaaatt tatgtgcact tacactcata aaatctatat tgtatttaga tagtctaaaa
     4141 ttaatttttc catatgactg aatagcatca acatggaaat aaattttttc ttttaaaccc
     4201 ttcaaatact ttccaacttc acctattggc tgtatagttc ctatttcatt gttaacatgc
     4261 atcatgctta ctaagcatgt agttgacttt atcgcatttt ttaaatcctc tatatttatt
     4321 ttcccatctt ttccgacctc taaataagtt acttcaaaac catcttcttc taaatctttt
     4381 aatgtattta aaacagaagg atgttctatg ttagtagata ttatgtgatt ttttcttttt
     4441 ttatttaaac tagtaatacc tcttattata gtattattac actctgtacc acctgatgta
     4501 aaataaatct ccttttcttt cgctcctaga gatttagcta tatcttgtct tacttctttt
     4561 atagcttttt caacttctac accttttcta tggacagaag aaggatttcc atattccgta
     4621 ttaagtgcat aaaccatttt atctataact tcttgataag gttttgtagt tgcgctattg
     4681 tctaagtata tttccaactt aattacacct acctttattt aaaatcttca actctaacta
     4741 ttataccact aattatagta ttttttttgt taatctttat taaaaagaca atcttaaatt
     4801 ataaaatatt atttcatgaa aagctagttt tttcacttta ttacaaattt ttatagtaaa
     4861 atataa
//
LOCUS       DAMKMI010000207         4860 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMKMI010000207 DAMKMI010000000
VERSION     DAMKMI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.213"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(339..1142)
                     /gene="proC"
                     /locus_tag="PC429_003547"
     CDS             complement(339..1142)
                     /gene="proC"
                     /locus_tag="PC429_003547"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF3849001.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1467..3836)
                     /locus_tag="PC429_003548"
     CDS             complement(1467..3836)
                     /locus_tag="PC429_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF3849002.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3841..4749)
                     /locus_tag="PC429_003549"
     CDS             complement(3841..4749)
                     /locus_tag="PC429_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF3849003.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1454 a          927 c          521 g         1958 t
ORIGIN      
        1 cttattagcg aagaaaatat ttgtaacttt tattacctta tgaatattat ttcaaattta
       61 aaaaggctat aaataaattt atttttaaat aaatctactt ataacctcac aataatactt
      121 ataaatttta ttttttcaca atgatatatc tcaaatatag ttaaaaatat atctatttaa
      181 ttattaatat ttaagtaata aattctcact caatcattaa attccttttt atagtaaatt
      241 actcttattt tacttatacc aaatacattt atgtatagat ataaataagc actaaaaaaa
      301 caattcaaga aaccatatag taaacttaat tacatctgtt atttactcat atctttagac
      361 ttatctatac aagcaacaat agattttata actgctgctc taaagccttc ttcttctaat
      421 accttaacag cttctatagt agttcctgct ggagaacaaa ccatatcttt taattcacct
      481 ggatgttttc cagtttcaag aaccatctta gctgacccca ttactgcttg agatgcaaat
      541 ttataagctt gttgtcttgg cattccagct aatactgctg catctgccat agcttctata
      601 aacataaata catatgcagg agaagaacca ctaatacctg taactgtatc cattaaatat
      661 tctgatacta tttctgcctc accaaaagat tcaaacagag ccttaatcat atttaactct
      721 tcatctgtta cattactatt tttacaaaga gctgacattc cttcgccaac aagggctgga
      781 gtattaggca ttactcttac tattttttta tctgaaccta taaaactttc cagactttct
      841 atagttttac ctgctgctat agatactatt attttatttg catctattaa ttctttaagt
      901 ggagttaaag ccttttcgca tatattaggt ttaagtgcta ctattaccac atcagaattt
      961 gtaacagcta cctttgaatc agttgtagta tttactccaa attcttcttt tactttatct
     1021 aaggcaactt ggcttaaatc tgatacagtt atatcttctg cctttactaa tcctgactgt
     1081 actatgccac ctatcattgc acttcccata ttccctgaac caataaatcc taaagttttc
     1141 atatcaaatc tccttttttc tattagtttt attttaaagt agctaaattg actttatttt
     1201 aactgcttaa aattatctta tttttataaa atataacctt attataacta taataaatta
     1261 ttcttttgta tgtattactt actaatatgt gtatgattta ctaaatataa atttaacgat
     1321 tttttcatta aacttaacga gctaacttcc tatttagaaa gttagctcat aattaatcta
     1381 aaattttaaa aatggaatca aaccatttat actttattat atcagttacg attttatata
     1441 gtaagaagtt ttatttacaa gcaaacctag aatgtttgct cagttcttcc tattatctca
     1501 tcttgtagtg ttctacttaa attattgaaa tggtcactat atcctgcaac tctaactatt
     1561 aagtctttat aatcctgagg attcttttga gcctctaaca gtacattttt atcaaataca
     1621 ttaaattgta tatgatgacc atccatatta aagtatgctc ttactagatt agccatatta
     1681 tctaaccctt tttctccttg aactactgat ggtgcaaatc tttgatttaa taatgttcca
     1741 ccagttttta tgtggtccat cttagcacaa gattttataa ctgctgtagg tccattagta
     1801 tctccacctt tttcaggtga aattccctct gaaactggtt ttgcacaaag tctaccattt
     1861 ggacttgctc ccataatttc tccaaagtat atatgacaag ttgttggtaa catatctact
     1921 ctgtattctc caccacaaac agttggtcta ccagttacct cattgtaata tgcattgaat
     1981 atctcttgca ttatttcatc agcataatca tcatcatttc catatttagg agtcttattt
     2041 gaaactaaat taagtatagc ttcatatcct tcaaaattag catcaagtgc tttaagcaat
     2101 gtatccatat cgaatttttt cttatcaaat acattatatt ttattgctgt taaactatct
     2161 gttatagttc ctataccaac accttgtata tatctagtgt tgtatcttgc tccacctctt
     2221 tggtaatcct tgcctttttc tatacaatca tcaactattg tagacataag tggacaaggc
     2281 atgtgttttg cacatatatt ttctatcaca gcatttcctt ctatttttat gtcaatcata
     2341 taatgtaatt gctttttaaa tgcttcaaat agttcctcat aagattggaa atttctaggg
     2401 tctccagttt caatacctat ttgtttttta ctaattgggt catatccatt atttaatgtt
     2461 aattcaagta tttttggaat gttcatatat ccagttagaa catatgcttc tttaccaaaa
     2521 catccagttt caacacaacc actggttcca ccaagtcttg catcttctat agtttttcca
     2581 gcttccataa gctcttgaac tattgcttca gtattataaa atgctggttg accccatcct
     2641 tttcttgata tttcacaagc tctctttaag aatttttgag gagtcttttt acttatttga
     2701 acatttgaac taggttgtat caatttcatt tcatccataa catcaagtat tatataacta
     2761 acttcattaa caccatcttg accatctgga tttattccac cagtgtttat atttgcaaaa
     2821 tctgtatatg tgctactttc ttttaatgtt ataccaactt ttggtggtgc tggctgattg
     2881 ttaaatttaa cccataaaca ttcaagtaat tcttgtgctc tatctctatc tagtattcca
     2941 ttttctacat ctctttcata gaaaggattt agatgttggt caagtcttcc tggagtaaaa
     3001 gcatcccata tattaagttc agtagtaacc cctatatgaa caaaccaata catttgtata
     3061 gcttgatgat aagtttctgg tttgtgagct ggtacaacat cacaagtttc agcaattagt
     3121 aataaatctt gtttcttagc ttcatctgtt tcttcttctg ccatttgtct tgctttctca
     3181 gcatatcttt tacctaatat aaccattgca tcacaagcta ttgccatagc ttctaaatct
     3241 gcttttttat tatatgcatc taaatcatta agaaaatcta gctcttttaa tcttgcttct
     3301 atgtctttct ttaaatctaa aaatcctttt ttatatattg tatctccaca aactgtatgt
     3361 cctggggctc tttgttccat aaactctgta aacatacctg cttcatatgc tgctaaccac
     3421 tcaggtgtca ttgcatttat tatcttatct cttgtttgtc tctttttcca aaaaggaatt
     3481 atttcttctt tatgtatttt tctagcttct tctgatacgc taaaatttat tatatctcta
     3541 ttgtgcatta cttctaaatc ttccattgta tgacaacaaa tttctggata tgttggtgct
     3601 ccattaggag aatcaccttt ttcacctact attaattctc catcatttat gctaagagtt
     3661 ctattctcaa tataatgctt aaatgataaa gcacgaagta caggaatctc tacacttccc
     3721 tcatattttt tataagcttc tgtcataagt actgctcttt caatagatat gtgtggttct
     3781 gcattaatac tttcttctct taatttttta gttctctcaa aagttcctct tgccatttta
     3841 ttaccctcca atcttagtat tgaaattact tgcttcaaac agtcttttta tttcctctaa
     3901 tttttcttct gatggtcttt gtagttcttc tccttcatac tttttataaa ttttatcata
     3961 cttgtgttta ccaatattat gatatggaag aagacttact gcttgtatat tcaaaggctt
     4021 taaaaactct atcatttttt taacctctaa attttcatca tctacattta ctcctacaat
     4081 taatggtatt cttatattta tgttagcccc taattcactt aaaatcttaa catttttcag
     4141 tattaagtca tttgattttc ctgtaaattt tatgtgctta tcttcatcaa taagcttaat
     4201 atcatataaa aacaaatctg cacatttagc aactctttca taattttctg actttgcata
     4261 gccacatgta tcaattgcta catggattcc ctttgatttg cacatgttta ttactccaca
     4321 aatgaagtcc atatcttgag ccattacttc tccacctgat agagttactc caccacctga
     4381 ttcttcatag aaaattctat ccttctctat ctctttaacc aaatctctta cagaatactc
     4441 ttgaccaaca atctctcttg cattgtttac acaatagtct aagcaagttt cacaaaactc
     4501 acacttatct tggtctaagc atatttttgt ttcacctttg taaattgcct tatgagggca
     4561 tatatttata catgcttcac attttgaaca tctttcttca ttatacaaaa cttgttttgt
     4621 atatgtttga ctctctggat tatgacacca aacacattca agtggacacc ctttaaaaaa
     4681 tactgtactt cttatccctg gtccatcatg tatgctacat ttttgtaagt ttataactaa
     4741 tggattcatg atttttcacc cctaaataat atccattcca ttttttcatt tttatgcaat
     4801 tcatcttcat agaatatacc tatgaaaagc atttctacaa ttacataata ctatatttgt
//
LOCUS       DAMKMI010000208         4765 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMKMI010000208 DAMKMI010000000
VERSION     DAMKMI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.214"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..646
                     /locus_tag="PC429_003550"
     CDS             <1..646
                     /locus_tag="PC429_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HDF3849004.1"
                     /translation="ATGATGADGATGPTGATGATGVTGATGPTGPTGATGATGATGAS
                     AIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTLTNFAFS
                     MPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVTLAPPLT
                     GILSVGSISSGIVTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIAIN"
     gene            815..1576
                     /locus_tag="PC429_003551"
     CDS             815..1576
                     /locus_tag="PC429_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF3849005.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1885..2163
                     /locus_tag="PC429_003552"
     CDS             1885..2163
                     /locus_tag="PC429_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849006.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2456..3031
                     /locus_tag="PC429_003553"
     CDS             2456..3031
                     /locus_tag="PC429_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF3849007.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3137..3970)
                     /locus_tag="PC429_003554"
     CDS             complement(3137..3970)
                     /locus_tag="PC429_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF3849008.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(4038..4667)
                     /locus_tag="PC429_003555"
     CDS             complement(4038..4667)
                     /locus_tag="PC429_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF3849009.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         1762 a          614 c          771 g         1618 t
ORIGIN      
        1 agcaacagga gcaacagggg cagatggagc aacaggtcca acaggagcaa caggggcaac
       61 aggagttaca ggagcaacag gcccaacagg cccaacagga gcaacaggag caacaggagc
      121 aacaggtgct agtgcaataa taccttttgc atcaggtata ccactatcac ttacaactat
      181 agctggagga ttagtaggta cacctggatt tgttggattt ggtagttcgg ctccaggatt
      241 aagtatagtt ggtggagtaa tagaccttac aaacgcagca ggaacattga ctaactttgc
      301 attttcaatg ccaagagatg gaacaataac atctatttca gcatacttca gtacaacagc
      361 agcactttca cttgttggtt caacaattac aattacagca acactttacc aatctactgc
      421 accaaataac tcatttacag ctgtaccagg agcgacagtt acactagctc caccacttac
      481 aggtatatta tcagttggtt caatttctag tggaattgta acaggattaa atatagcagc
      541 aacagcacaa actcgattct tactagtatt tactgcaaca gcttcaggtc tttcattagt
      601 taatactgta gcaggatatg caagtgcagg aattgcaata aattagatta gataagaaaa
      661 ggcttctaat atcgtattga tattaggagc ttttttaaga atgattgatg aacatagttt
      721 taatattaat tagtgcgcat attataaaat actattatat aatataaaat aataacatta
      781 aattggaaat atagaattat ctaggggtga tactatgaaa aataagattg aaatatcaat
      841 gaacaacttt gtaaaaaatt atcataaatc aaataatata actacaaagt ggcaagaact
      901 tttgtttgct tatgcatctg ctgaagatga tatgtttcag aagctaaaaa gtgttgtagg
      961 aacatctcat gctatgccaa aagattttct ggaaaatgga aaaactataa ttacatattt
     1021 tatacctttt gatgaaagtg taaataatag taatattgga gggtttgaat gttctgaaat
     1081 atgggcaaga gcgtacatag aaactaatat gttgatttca aatttaaatg atttcttatg
     1141 tgaagaacta gagaagatag gggctaattc atatgctatt cctgggtcgt ataattttga
     1201 cagtgaaaaa ttggtaagtg actggtcaca aagacatgta gcattcatag caggtcttgg
     1261 aacttttggt ttaaataata tgcttattac agaaaaaggg tgttgtggaa gaataggaag
     1321 cataatcaca agtttagaat tagagccaac taaaagacca gaaaatgaat actgcttgta
     1381 taaacataat aatagttgca aaaaatgtgt caataaatgc gtgaaagaag ctttaaaaga
     1441 agatggattt aataggaata agtgttatga aatgtgtatg ataaatgaaa aaaaatacac
     1501 ttatataggt ggatgtgata tttgtggaaa gtgtatagtt ggagttcctt gttcagtaat
     1561 aaacccatca agataatgga tttaagttga ctaataattt taaagatagt ttataatgaa
     1621 aaaattttag ttatcaaaat aaatataata taaaaactaa ataaatgtgt ttaaaaaatg
     1681 gataaataat ttgtgttgat tgacaaccag ttatactgtt gatagactta tatgtatcgc
     1741 tgtgatatat aaggagggtt gagattttat gtttgattta atctttattt ttgcatagca
     1801 agggctattg caactatgaa atttaatata taaatagccc tttgctattc ctaatgaaaa
     1861 attactattt aataggagag caaaatgagc tataaaaaag caatacatat attaccagaa
     1921 gaattacttg aattaataca ggaatatgta gatggtgaat gtatctatat tcctagaaaa
     1981 tctaataata aaaaagaatg gggaagtaat acacctactc gtgaggagtt gtcaattaga
     2041 aatatgcaga tttacgaaga ctatcaagtt ggatatgatt tgcaatattt atcggaaaag
     2101 tattatttat ctttgaaaag tatccaaaga attgtattac aagaaaaaag aaaagatatc
     2161 taaggatgag ccagtatagt tttataatta tactggctca ttttcttttg ataaaagtgt
     2221 ttgaggtata gtttctttta agtggatgat ataatttaag taatttcaat aacttataca
     2281 gtagctaaca aagctggttg taaagtatct aataagaagt agtagctttt tatatgtttg
     2341 aaattaaaat ttattaaaat taggaggtct tgttttgaaa aaaatacttg attatattcc
     2401 accttttcat aactttctat taatttagta atcttataga aaggaaattg catttatgaa
     2461 aaaaattact tgggaggtgg aatatatctc ccagttacct gtaattaggt attgtaaaaa
     2521 atgtggaaaa aagactgaat atatttgttc aggtttattt agggttaatg ctcaacataa
     2581 atatttggat atatggttaa tatacaagtg ttcaaattgt gattcaacat ggaactcaac
     2641 tatatattct cgtgttaatc caaaaagctt aaatccagaa atactagaac aattccatac
     2701 aaatgattat aatcttgtta agcaatatgc tatgaatatg gaacttttac atagaaatgg
     2761 agcagaagtt ggtattccag agtataaaat agtaggacaa gaaattaata ttaatgacct
     2821 tacacaagtt catattatta gtaaatatcc atgtcagtta aaggtatcaa cattattacg
     2881 tgagaaacta ggtttatcta ggaaggtatt tgacgaaatg ttggattgtg ggattattag
     2941 aagtaactca ggagttgatt taaagaaagg taaattaaat tatgaaatta tgttagatat
     3001 aggcaaggag aagcaaaagt cttcaaattg attagaataa ctaacttaat atattttcag
     3061 gaacatcatt taaaaaaata gtgactttaa aaaatttaaa gccactattt tttaatatgt
     3121 tttatctata taactattat ttttctaggt tttttaatat ttcgtaatag tgtgttgtgg
     3181 ctatttcact tgactcgcct tttttacaaa aagataattt agatagtttt ggaactacac
     3241 gttcaacgac aattttatta cctatcattt tagattcaag tatcttcaat gcttctaaaa
     3301 atctcttatc cttttttgca cgattataaa atgataaaac gtagacataa aggaataggt
     3361 tataattacg gaaaggatat tcaacttgca taaataatgt gccaattcca tagtgacaag
     3421 ggccgattgg ttttttaata gtccaatggt caagtagaaa atctacagct ttgtcaagtt
     3481 ttgattcttt atttagataa ttactaaaac ggaatgcatt taatatatta agagttggta
     3541 gtggatttga gtattcagtt tctggacctc gaccaaaact aaatttattg caacgccacc
     3601 caccatcagt atactgtgta tctaaaaaat gttgaaacgt tttttgcatt ctaatgtcag
     3661 atgcatatcc catatgacag agtacgttag cagcatttgc agtatgacaa gggtaaatac
     3721 ctccatttgg atatagtttg aaacgtccat cttctcgcca tgtgctaaat atcaggtcag
     3781 cacattcttt aagtaaagta tcatcagggt tcacacctaa ttctaataga taaagtacac
     3841 tatcaagagt agagaaagga gaacctttta ttaaacgctt atcaggtgta gtccaatagt
     3901 ctgcaccatt atcatacctg tgtgataata ttatttcaat atctgataaa gattgatttt
     3961 ctacattcat tttttactcc atttcatatt atattctatt aaatctatca aaaactttat
     4021 tataatatta tttatattta taattgattt attttattta aaattgcatt tatagctgta
     4081 tcaggaacaa ctgctaaatc ttctgcattg aagtaataaa ctatattaga atcaaataat
     4141 atatttgaat ttggaggaaa ttcatcatct ccaaaccaaa gtataaatac catagatgta
     4201 ttacccatat atgtaaacct ataagctaaa tctcccatgt ttactttttc agctccaatt
     4261 ttctccataa ctaacttata tttgtctatt tgagatgtaa aaacttctga aagcctatat
     4321 agagtcctgt ttaaaaaatt tggataataa acattgcctc ctggtatttc tttaaaagtt
     4381 acatatttat tagttctagg aataccttta gcattgatta aatatctaac tatcaagatt
     4441 ttaagctcaa agttattgca aaggctacca ttactattcc aaacttctcc acttggatag
     4501 tctataatat attgtctatc aaaaaaattg agtgtgaatc tatttaaatc attattgaaa
     4561 ggtatattac ttaaattact aattgtatga gggtctaatt ctttgagttt ttttcgaaca
     4621 tagtcaaatg gaacattata attatttttt gttatctttt catccataat tttttcctcc
     4681 aatttaaaca attaaaagta tatttataaa aagttttctt attattaatt ttattatata
     4741 ttcaaaatat tacaataata aattg
//
LOCUS       DAMKMI010000209         4672 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMKMI010000209 DAMKMI010000000
VERSION     DAMKMI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.215"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            200..1339
                     /locus_tag="PC429_003556"
     CDS             200..1339
                     /locus_tag="PC429_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF3849010.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1365..1553
                     /locus_tag="PC429_003557"
     CDS             1365..1553
                     /locus_tag="PC429_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849011.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2338..3894
                     /locus_tag="PC429_003558"
                     /pseudo
     CDS             2338..3894
                     /locus_tag="PC429_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
BASE COUNT         2055 a          494 c          789 g         1334 t
ORIGIN      
        1 taactaatgt attataaata atagttgtaa acttcctgtg acatatattt taaatgctta
       61 ttcttaaatt tttgcattat ttattaaaat ttattatgct ttaacgctaa attgagtttg
      121 ataattataa aacaaataat caaaaatagt gtaaaaatta atactttttg ttaatattaa
      181 tagataaaga taatctaagg tggtgattaa actgacaata gaagaataca aaaacaaata
      241 ctttactgga aataaaatcg tactttctat aaatagtttt gataataaag aactatacta
      301 tcatgaaact gaaaatatat taaatttaat aaatgaaata gataaattaa catcaagtaa
      361 gatatttaaa acaatagaag ataaaaatga tttggcacta gagtcttatt attctagtgt
      421 aatagaaggt gctttttcaa ctagaaagat tgcaaaatct ataattagag gaaagatgaa
      481 accaagtaat aaaagtgaat atatgattta caataatcat agagccttag aatatggatt
      541 agacaactta gataaattat atagtcacaa atttatatat gatttgcatc atatattggg
      601 cgaaaactgc ttggattcag aagaatatga gtatagaact gaaaaagtat atgtatgtga
      661 ttcaaaaggt gagattatac atacagggct tgaacctttg aagatatatg actttatgag
      721 taaattaata gattttatgg aaaatagcaa agtaagtaat ttaattaaaa gtgctataat
      781 tcatttttac tttgtatatg ttcatccttt tagtgatggt aatggaagaa catcaagagc
      841 cttatcatat ctatatttga ttgataaagg atatgatact ttcaaggatt tttcaatatc
      901 atatatgata tctaaaaaca gaactaagta ttataaggct atacttgatg tagaaaacaa
      961 aggaaataat ttaactgtat ttatagaatt tatgctcaag tctataatac aatctattaa
     1021 tgagatgcgt aatatgcatg atagaaaatc tttagaaagt atattaaaag aagaattgtt
     1081 tgaaaatgac ataactctat cagcaacaga agaacatata ttaaaatata tttgcaataa
     1141 agataactat tctatgacat tagaaaatta tataaaaaag aataaaagta gatacttaaa
     1201 ggctggaata aaggaaattg aattagttga ccagttgatg gaagtgttta ataatcttga
     1261 agagatagag attttgtcta aagaaaaaaa tatatataaa gttaatgata agtacttaaa
     1321 aatgcttgat atagactaat ttagataatc tggagaggga atatatgaat aataagaaaa
     1381 aggcattttt gatttctggg gctataaacg taatttcatt aattattgtt ttttcattgt
     1441 ttcattataa tattatagaa aatagtacac aaaatatgat aataactgta atcttaattt
     1501 ttatttttga aatgataaaa ataaaaatca tagagaaata ctataaacta taaacaagaa
     1561 atatcttttc taagagcata ttgtacaata tgctcttaat ttagttatat agatttaata
     1621 taaataatgt attttttgta gatgatttat gtaagattta tgaccaattt gtgtatgatt
     1681 tgtgttcttt aatcatgaca atattatatt gtaaaaaata aatttaataa aaaatgactt
     1741 ggaatttata ttctaggtct tttttatata aaaatttaat aagtttcaaa tgttggtaac
     1801 tttgacagta gaaaacgtct attgagataa cgaggattct aattgagggg gagagatatt
     1861 agcacttaaa gtagtatatg caacagatat aatctttaca aaagtaattt gaattaaaat
     1921 gtaaatataa agaataatta aaaaaatatc gcaattattt gtttttcata tggataaaat
     1981 tggttattta atatataatt acaagtataa ataataaaat ttatttgtaa aaagagtagc
     2041 acttttaatt cataaaggct acttttttta tacaaaaccc tattttttat aacaattcat
     2101 taacataaaa atcaaactta ccttaaaata taaatttaca aatttttcaa tcaataagaa
     2161 aatttcttta ttccttacat aatgactgca atgtaacaaa ataattacaa attaattatg
     2221 aaatcattat agttagaaac ctctttaaaa ttagatatta agtatccttt agaattagaa
     2281 cgggaacaaa gttaaaaaat taagtaatat caaatggaca agggggaaga aaataaaatg
     2341 aaaataccaa agaagattgc tgcaatgctt acagttacaa tgatagctgg aagttcaaca
     2401 gcaggcatag ctagtgcaca aactgtggca acaaatttaa cagggcaaga aagatatgaa
     2461 actgctgtta aaataagtca agatggatgg aaaaatgctg atgaggtagt aatcgtaaac
     2521 gactcatcta tagcagatgc tctatctgca acaccatttg caaaagcaaa aaatgctcca
     2581 atacttttaa caagtaaaga taaattaaat gataagacga aagcagaaat acaaagatta
     2641 aaagctaaaa aagtatactt aataggtgga acttctgtat taagtacaaa tatagaaaaa
     2701 gaaataaaag atttaaaaat atcttttgag agaatctctg gagctgagag atatcaaaca
     2761 tccttagaat tagcaaagaa attagatgct ataagtgatg ttaagaaaat agctgtagtt
     2821 aatggtgaaa aaggacttgc agatgctgta agtgtaggag caccagctgc acaaaacaat
     2881 atgccagtaa tacttgctga ttctaaaaat ggaacagcag ttgcagacaa atttataaaa
     2941 gatgctggaa taactcaatc ttatgtagta ggtggagaga gttcaatttc tgaagctgtt
     3001 aaaaataaac taccaaatag tacaagacta ggtggaacag atagaaatga tacaaatgca
     3061 aaagtaataa aagaatttta taaaaagaca gatttaaaaa atgcttatgt aactaaagat
     3121 ggtatgaata aacaagacca acttatagat gcactagcag taggtgtatt aggtgctaaa
     3181 aatcaatcac cagtagtttt agttggaaag aacttatcag cttcacaaaa atctctagtt
     3241 aactctaaga gttttgataa gataactaaa gttggtggga atggtaatga gacagcattt
     3301 aatgaaatga agtcattaca agaagtaaaa acagttgaag ctaaaacaat ttctgaatta
     3361 aaatcagcta tagataaagc tactgctaat gatgttataa actttaagcc aactagtgag
     3421 gttaaagaag catttacaat acaaactgat aaagcagtaa cagtaaactt aaatggtaca
     3481 tatacaaaga ctgtaactat caacatgcct aatggagatg taaataacta tgcaaaagtg
     3541 gatgatgtag ttatagatga tgtaaaagat ggaacttttg ttaactatgg aaaaataact
     3601 aacttaaagg ttaatgataa aaatggagcc aagatagaaa ataattctaa aggtgagatt
     3661 ggtagtttaa cagtagcatc tggagcaagt caagtaaaag tgactaatgg tggtaagata
     3721 actacagtta caaataattc taaagggact actatagata ataaaggtac aataagtagt
     3781 gttaagggag ataattctcc aacaataagt ggaaatagtc caagttctaa ctcttcagga
     3841 ggaagttcat caagtggagg ctcttcacat ggaggaggaa gttcttcatc aaaaccaagc
     3901 gataagtcaa aaacaaagac aaaagcagaa gaaaaaatag cagaggtaac aaaactagta
     3961 ggaaaagtaa cagcagataa tttagaagca tcaaaagtaa cattaaaagc agcaactgat
     4021 gcaatagaag aagcaaggaa agcagggtgg acagaatcag aagttcaaag ctttaaagga
     4081 tatggagata tagcaaaagt acaaagtgag ataactgaat tagaatcaca ggctaaagaa
     4141 gcagcaaaga caaaagcaga agaaaaaata gcagaagtaa caaaactagt aggaaaagta
     4201 acagcagata atttagaagc atcaaaagta acattaaaag cagcaactga tgcaatagaa
     4261 gaagcaaagg cagcaggatg gacagaatca gaagttcaaa gttttgcagg atatgaagat
     4321 atagcaacag tacaaggtga gatagctgca ttagaatcat cacaggctaa agaagaagca
     4381 aagacaaaag cagaagaaaa aatagcagaa gtaacaaaac tagtaggaaa agtaacagca
     4441 gaaaatttag aagcatcaaa agtaacatta aaagcggcaa ctgatgcaat agaagaagca
     4501 aaggcagcag gatggacaga atcagaaatt caaagttttg taggatatga agatatagca
     4561 aaagtacaaa gtgagataac tgaattagaa tcacaggcta aagaagcagc aaagacaaaa
     4621 gcagaagaaa aaatagcaga agtaacaaaa ctagtaggaa aagtaacagc ag
//
LOCUS       DAMKMI010000210         4639 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMKMI010000210 DAMKMI010000000
VERSION     DAMKMI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.216"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            111..1280
                     /locus_tag="PC429_003559"
     CDS             111..1280
                     /locus_tag="PC429_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF3849012.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1443..1985)
                     /locus_tag="PC429_003560"
     CDS             complement(1443..1985)
                     /locus_tag="PC429_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF3849013.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2133..2417)
                     /locus_tag="PC429_003561"
     CDS             complement(2133..2417)
                     /locus_tag="PC429_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3849014.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2480..3244)
                     /locus_tag="PC429_003562"
     CDS             complement(2480..3244)
                     /locus_tag="PC429_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF3849015.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3526..3792)
                     /locus_tag="PC429_003563"
     CDS             complement(3526..3792)
                     /locus_tag="PC429_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF3849016.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(3834..4589)
                     /locus_tag="PC429_003564"
     CDS             complement(3834..4589)
                     /locus_tag="PC429_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3849017.1"
                     /translation="MPHKLLRQYIAHYTISIPDTSIKENLTLIPDASGCMIFKFDKKG
                     IESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTGLSQRESTDLKISLEDFNT
                     LFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMTIPILENARKQNSIMSVKN
                     ISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEIQNNKIPFATLAQDIGYYD
                     QSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1572 a          647 c          652 g         1768 t
ORIGIN      
        1 aggaactacg gggatagcct gtggagaatt agtaagacat attttagtat gcaaaattct
       61 gtgaagcagg aaccctgtga ctttagtcat gggaggttca gggtagaaag ataaatgaac
      121 tttgtaaaga tgaagttgta gagttttgca atagaataaa aggtaaagtg gaaagtgttg
      181 caataagtgg agtatttgct ccagtaaata aacaacaaga agaagaagtt gcaaaatggg
      241 ttagagaaat tttaggggat attcctatat ctttatcaaa tgaaataggt aatataggta
      301 tattagaaag agaaaatgga gctattttaa attctgcatt gtctcaagta ggtaaagctg
      361 tatctttagg atttgaaaaa gcacttaaag acttaggtat agatgctcaa atatatttca
      421 gtcaaaatga tggaacatta atgaatttag agtatacaat gaaataccca atttttacaa
      481 ttggatgtgg aataacaaat tctataagag gagcttcctt cttatcaaaa gtaaaaaatg
      541 caatagtatt agatgttggt ggaactacaa gcgacttggg tgtactatat aatggattcc
      601 ctagagaatc atctattcca gttacaattg gaggagtaca tacaaacttt agaatgccag
      661 atgttttaac tattggtctt gctggtggta catgtgtaaa aggcgaaaaa gataatataa
      721 agataggtcc tgaaagtgtg ggatatagaa taactgaaga tgcaataata tttggtggag
      781 acactctaac tttatcagat gttgttacaa aattagatat ggcgtttgaa gatagaaaaa
      841 gcaatgttga aaacttagat aaagagtatt gtaatgaggt atataagaag gttattttaa
      901 aattagaaga tgctgtagac caaatgaaga ctagtcaaga tgatgttgaa ttggttttaa
      961 ctggtggagg aagtataatt gttcctgaaa aactaaaagg cataagcaat gttataagac
     1021 ctcctcatta tactgctgca aatgctattg gtgcagcttt aggacaaata agtggagatg
     1081 tagaaaaagt atacagttta gataaaatgt cgagagaaga tgccatagaa gatgcaaaaa
     1141 aagaggctgt aaataaagct ctaaaagctg gtgctaaaga agatactata gaagtattaa
     1201 atatagaaga tgttccactt gcataccttc ctggtaatgc acttcttata aaagtaaaag
     1261 ttgttggtga tttaatttaa agaataaata taagtatttc cagaattata taaatatttc
     1321 tggactcatt tttaaactta ttttatcaat aaaaacagca taacttttga ctatattctt
     1381 ataagtttcc gaacagccga tatataccta ttttttgagg tttatcggct gtttgttatc
     1441 ttttatcgca ttccaactat ttttcttacc gtatttgctg tacgaattgt aatcttatca
     1501 cttacatttg aactggcagt ctttgaccac caatttgtct ttttataatc tttacgacta
     1561 aaagaccaaa agatagcatt tttataatga tatacttttt catactcttc tttaggattt
     1621 ccaacctcat cataaaactc gtcataagat aagtcaggaa acataaaaat aagattatca
     1681 tatatttctt tgttgttatc tccccaccaa ttaggagcat tatttagtat atcttttaac
     1741 tcttcttgtg aaataataaa aactggtatt tccaattcaa actgtttttt tatcattaat
     1801 cttattgtat ttgagataac cttgctgtca tctacatcac ttaaaaagac aacattacca
     1861 ctattaaggt atgtagaaac ttctgaataa ccaagttcta caaagcttgc ttttaattca
     1921 gacataatta tcttgttctt accacttata ttgatacctc ttaataatgc gatataactt
     1981 ttcatataat aatttcacct ctaaacattc acatttttct atgtagtaca aactggaatt
     2041 tgcatctttt ataattcttt caaaacttct tcatatgtaa tttttttaat ataaaaaaat
     2101 cggtgcttaa tttgtaccga tttttgtttt tcttagtaaa gaactcgttt ttttgcttct
     2161 gctatagctc tagcagcact ttttgcttgc attctttttt cttcttcttt tttaaattca
     2221 tttattgttt catttagatt cttatcatgt tcattttttt ctttatcttc gttttctctg
     2281 tatactaata tataatctat tattgattcg tttattattt gagatatact tttattttca
     2341 ttaattccta ttctcttaac ttccttaagt agagtctcat caatatgaac cgttgtttgc
     2401 tttgtatttt ttgccacctt aatcacctct taaaaaaaat tctaacatta aaattttttt
     2461 tagtcaataa attttaatat taaaattctt taaatgtttt atttgatttt ctaaaatttt
     2521 ctattgtatt tatcatctta ttttttatat tttcacctag tacagaagac tttagtctag
     2581 gattttcgat atggtcataa attggttcta aaaattcgac ttcgacagga acagagttaa
     2641 ttttaccaat aggttggtat ccttcataag tatcttttga attttttatt actagtggaa
     2701 caatagggca ttttgcttta tatgctatct ttaaagctcc acttctaaac tccgaaacca
     2761 atgaatttgg ttctttaatc catgttaaat ctccttctgg aaacactgcc atactttgac
     2821 ctgtaagtat attttgagag gcttgggcta tactctttat accctcacga ttgttttttc
     2881 tgtttacata tacacatctt agtaattttg cccattcttt aaatataggt atatttctcc
     2941 aaactggttc atctgcaatt acacatccaa ttggtctttc tacacttgct gttagtatga
     3001 agctgtcaag catactagaa tgatttacaa caaatagaag tggttctttt ggcagtgttt
     3061 ccttaccaat tatattaagc tctatgttca ctatatctaa agatttttta gcttgttttt
     3121 gcataaattc aaatttttcc tttaagctaa atttatcagg attcttttta attttaataa
     3181 gcctaggtat agaagatgta aaacctataa gttgatatgt agcaaatttt aagtaattat
     3241 tcataaaatt atctccttaa aataaaatta tttattactg taaagatttt aacataaaat
     3301 aaaatttaag tctattttaa ttcaattttg taacatatat tttaaataga cagagattga
     3361 gattttatat ttccaatttt agtaatcaga ttttatcaat aaattactat tctattaatt
     3421 taattaaata acctttaaat ttaattataa aaactgttgc agaaatttct acaacagttt
     3481 aaaaaattta ccttacaaaa acttatttta taacaagaga gtatattata aggctagtac
     3541 agcccatttt ttgcactgag aaattctttt gccatcatca ccttgttctt tgtcatatgc
     3601 aaattgatta agcatttcac atggaaatgt atcacataga ccacaatgtg taaggttttt
     3661 atcttcacaa caagatttta caggacattg ttcaccccaa aatggtttct caatctctgt
     3721 acaaccttta caattgactt tttccttgta tccacattca taacataaaa ttccacatct
     3781 tgattctatc attttaagtc actccttaaa tataaatttt atatgattgt atatcaaaat
     3841 ttatactttt cattgtaaaa atcggacaaa ttttttatat aggttgttgg attaacacca
     3901 caaatagatt taaaatcatt tataaaatga gattggtcgt aataaccaat atcttgtgct
     3961 aaagttgcaa atggtatttt attattttgt atttcttgta atacaaggtt tatacgaagt
     4021 aatctaaggt atgatttaac actcatacca agagaattat taaaaattct atttaaatgt
     4081 cgttcactat aacaactaat ttgtgaaatg tttttaacag acataataga gttttgtttt
     4141 ctagcatttt caagaattgg tatagtcata tctactatat ttgatttcaa taagtatgat
     4201 aagaataaca tatctaattg ttctactaat tcttctatag tacttgttct ttcaaaaatg
     4261 gaattaacct ctaagctaaa taatgtgtta aaatcttcaa gtgaaatttt taaatctgta
     4321 gattctcttt gagaaagacc tgtcaaataa tatactccac ctgggcgaaa ctcaacgaat
     4381 actctaaaaa gtacattttc tatatcattt tttacaatag tagtttttgt tgtggctccc
     4441 cagaatgcgc tttctatacc ttttttgtca aatttaaata tcatacatcc actagcatca
     4501 ggtattaatg taagattttc tttaatactt gtatctggaa tagatattgt atagtgtgct
     4561 atatactgtc ttaacaattt atgtggcaaa atatatgtat actgtttgca ggtatttaaa
     4621 atgttattga ttattttat
//
LOCUS       DAMKMI010000211         4588 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMKMI010000211 DAMKMI010000000
VERSION     DAMKMI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.217"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            69..1235
                     /locus_tag="PC429_003565"
     CDS             69..1235
                     /locus_tag="PC429_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF3849018.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            1352..2134
                     /locus_tag="PC429_003566"
     CDS             1352..2134
                     /locus_tag="PC429_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF3849019.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(2253..2906)
                     /locus_tag="PC429_003567"
     CDS             complement(2253..2906)
                     /locus_tag="PC429_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3849020.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(2930..4294)
                     /locus_tag="PC429_003568"
     CDS             complement(2930..4294)
                     /locus_tag="PC429_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF3849021.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         1702 a          600 c          671 g         1615 t
ORIGIN      
        1 tggtatattt gttataaaag tggtgatttt gtgataaaat ttaattatat agaaataagg
       61 aggaaaaaat gaaaattaat tggaatacaa aatacacaac aatagcaatt tatacattta
      121 taatagctgc tagtagtatt atattttatc tagtatcatc acaaattgat gtattttcta
      181 ataatttaga tgctatattt acaacattac aaccttttat aataggattt gctattgcat
      241 atcttcttaa ttttatttta aagttttatg aagacagaat ttttataaaa agtgagaaat
      301 taaaaaaact aaaacaaagt tcaaaaagag gcttgggtct tttattaaca tatgcaacag
      361 ctacattgat actttactta tttatgcatt tcgtattgcc acaagtaatt gagagtattg
      421 ttggactagc aaatgatata cctatgtatg taaacaatgc aactaagctt attgataaat
      481 taatgactga tttaaatctt gatgagcaat actttaattt ggcagttgat aagtggaatg
      541 agtttgttac atatattata aagtttgtaa cagatttaat cccaatatta ggaagtatgt
      601 taaagaatgt agcctctagt atatggaata ttgtactagg attaattgtt tcagtatact
      661 tattaattga taaagaaaaa ttctatggat taagcaaaaa aataacttat gcagtattta
      721 cagaaaaaca agctgccaga attttagaat taactcatag aagtaactat acatttggaa
      781 gatttttagg tggcaaaata ttagattcat tcattatagg tattctaaca tttgtaatac
      841 ttacgttagt taaaatgcca tatacattat taatatcagt tataattggt attacaaata
      901 ttatcccttt ctttggacct ttatttggag ctataccatc tacgctaata gtgatgtttg
      961 tatctcctat aaaagctttc tggttattgt tgattatatt aataatacaa cagatagatg
     1021 gaaatattat aggacctaaa atacttggag attcaattgg tatatctgca ttttggattt
     1081 tgttctcctt attggttgca ggaaaattat taggttttat tggaatggtt ataggagttc
     1141 caatgtttgc agtaatttat tctataatta aggatattgt tgaaagtaaa ttggataaaa
     1201 agggattacc gacagatact tccgattata tgtagctaaa aaatattatg tataattaaa
     1261 aatatataaa ttaagcaata tttaaagcac tgagaaagtt aaactcttgg tgctttttag
     1321 atataaaatt attatagaag aggtgttttt tgtggcattt atagaatgta aatttaaatc
     1381 taaatatact ggaggagaaa ctgatataat tgtaattttg cctgtagcaa atggaaaaga
     1441 actttttagt ggtaaagaga tatatgattt tgaaggtaaa tttaaaacat tatacttatt
     1501 tcatggttta gaagatgacc aaagctcatg gattagatat actaatgtag agcattatgc
     1561 taaaaataaa aatatagccc tagtaatacc aagagtagaa actagttttt ataccgatat
     1621 ggcgtatgga caccaatatt ttacttttgt aagcgaggaa ttacctaaat ttgttagagc
     1681 tatatttcct ttatcagata aaagggaaga taactttgta gctggtatgt ctatgggggg
     1741 atatggagct ttaaaattag cgctttcaaa accaaaagag ttttcggcag ttgcaagttt
     1801 ttctggttcg cctgatatga ttcatgagtt agaaaatcaa caaatagaaa ccaatgcaga
     1861 cattctattt cacgattttg gtagaccaga agacgctaga aatagtgaga atgatttact
     1921 ttacatactt aagaacctta aagaaagaaa tgaagatatt cctaaaattt atcaattttg
     1981 tggtgataga gattttttat ataaaaataa tcagatgttt aaaaaatatg cagattctct
     2041 gggtgtagat gttgaatatg aagagtgtgt aggggggcat gaatggcgtc tttggaattt
     2101 atggataaag aaatttatag atataattgg atagtacact tatggtatta acaggtatac
     2161 tataaaatat ctttaaatat gataaagact gaaatcctaa aatcaaatta tgattttaaa
     2221 atttcagtct ctaagaattt aataaaattt tattaagttc ttacacgagt tattattgga
     2281 gtaaaattgt caactatatc tatatagtat ttactcaatt cctctaaaat ctcttcgcat
     2341 aaatccgtac tatgaccaag attaataaga aactgttttg taaaatgctt tattacagct
     2401 tgagaagata tatcgtactt gtgtgaagta taaactctaa ttatttcagg tttcatagca
     2461 agtataccaa tacaagaaag tattaattca tcatgttcta tagattcttt atcattaaca
     2521 tatcttttca tgaccatttc ggttacactt ctttgatagc ttaaaatagc ttcttcataa
     2581 gctatatcca aataaaaatt tctatatgct tcgtttttta gaaggaaaca aaggtcagca
     2641 ttcatagcaa gaaagaacat catcatagta ccttcttcat ggtagttata gattaaagac
     2701 tgcatttcat ttcttatatc ttcatatatt atagctccta tttcgctttt acttgagaag
     2761 taatagctta ttagacctaa atttatatta gctctttctg caatttgcct agttgttgtc
     2821 ttatgataac cttgctcaaa aaataattct ctagcagcac ttataatact agcttttgtt
     2881 tctttgccct tttctgattt tgacatattt actgtgtata cctctctttt caatcttttt
     2941 ttgaaaattt gctaactata aatattgtaa tagaaccacc tattatagga accaaaattc
     3001 tgtcaaatac agtaagaata ccaatcatat ttaataatgc caatataaca cttactataa
     3061 cagcatagat agctcctttt tttgtagcac ctttccagca aagaccaatt acaaatggtg
     3121 caaatattac acctgtacat aatggagctg gccaaaacaa taattctaat aaactatcaa
     3181 atttaagtgc tatgattgca cctattatag ctgtcaaagc tgatgcaatt tgagaatact
     3241 ttagagtatt ttttaaatca ttaaattcta tattaggatg taataatttg ttataaatat
     3301 ctcttgatat aatagcactc attgaggtca ttaatgaatc ggcagtagac attatggcag
     3361 ccaatatagc agctaagaat aatacagata ttaaaggtgg caaagtagtc gatatcattt
     3421 gaggtaaaac ctgaacagat gatatatttg gaaataaact accaccaata gctccaatta
     3481 ttcctatagg gaaagcaaga aataatagta taaatcctgc aattacacta cttttaactg
     3541 catttttagc attatcagca cattgatatc ttaaaaaact ttcataagaa aatattgagc
     3601 ctaaaactaa tggtaaaact gtatacataa aatcactaat cgatttattt cctggaaaga
     3661 atggttcttt taccattgat tgtaacataa gtaaagggtt gtcgccaact aattttaagc
     3721 ttatataagc tactattggc aaaaacacta tacaaatagc aagttgaata aatgctgata
     3781 ctgcaactcc ccacattcct gatgtataag tatatataat gataactata actcctataa
     3841 ttacagctat atcataagga ataccaaatg cagtagatac agaaccaaaa gaaatcaatt
     3901 gactacccat aatagctagt atagatattg gtgttacaag actttgataa gctcttaaaa
     3961 atacactatg accatatcta gtttcaatat aatcagcagg tgttatatca caagataagc
     4021 ttcttaattt tcctgcgaaa aatgcattta taaagcaagc taaagctgca cctatagtag
     4081 aatacattcc aacccaaagt cctctttcag ctccccattg aactccacca actaagcacc
     4141 ctcctccaaa agatgtagca gcaatggttg cagctagtat aggcaaaggc agatttctac
     4201 ctgcgattag gaaatcggat gctgttttaa ctttttttga aaaataaaaa ccaatccata
     4261 tgtatattgc acacgtaaca attaaaagta acatagtctt ttctccttta taaaatattt
     4321 taataaaata tacaaaatat atttaaaata tattttatat aaaagtagta cgatatttgt
     4381 tttattggtt ttagattaat atagctataa taatttatga tattagatgg aaattacgta
     4441 aaaatagaaa attgaaaatt ctgaaatttt tgtatattga tatttatctc atagattctt
     4501 ttttgtaatt gtaacacgct tttattttta aatcaatagt acatgtataa aaataaaagg
     4561 aatttaaata agaaaaagat atttttaa
//
LOCUS       DAMKMI010000212         4547 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMKMI010000212 DAMKMI010000000
VERSION     DAMKMI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.218"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            514..1821
                     /locus_tag="PC429_003569"
     CDS             514..1821
                     /locus_tag="PC429_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849022.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1907..2169
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2295..3098
                     /locus_tag="PC429_003570"
     CDS             2295..3098
                     /locus_tag="PC429_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3849023.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            3136..3747
                     /locus_tag="PC429_003571"
     CDS             3136..3747
                     /locus_tag="PC429_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF3849024.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3779..4450
                     /locus_tag="PC429_003572"
     CDS             3779..4450
                     /locus_tag="PC429_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF3849025.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1822 a          417 c          821 g         1487 t
ORIGIN      
        1 atactaaaag ggaatattta cagtttaatg gcagttttaa aataataaat taaagctgtc
       61 atttttattt aaaaattttt agaaaaagta caatagtgtt aatgtgtgaa ttagataagg
      121 tgtatataat agtttagatg atatgatata aaatatgttg taaattaaat gttttctttg
      181 acaatatata cacaaggatg ataatatcta tatgaataaa agatagtgtg taatttgata
      241 atatgtaatt ataacattat gatataatta caataaagta tgtaataatc tgtagaaatt
      301 tcaagctagt tatctaagtt ttcggataac taaatctaaa cggaatacat aatgtattaa
      361 ataaatgatg atactaaaat gatactattt ttatagaaat ttattgaggt atataattaa
      421 tgaaaataat atattaaatt atataaatta aattatataa attaaattat ataatatatt
      481 gagttttaca taaaagcaaa ggtgggaata aaaatgaaag aaattgctga tgctataaca
      541 tcagagtatt taagctatgg tatcaatgaa gatattagta tctatgaagg tagattttgc
      601 atatatttag ataaaaaata tagatgtaat gggaaaattt attataaaat gacacctcca
      661 atatctataa gttttaaagc agacataggt tgtgttgaag agatagacaa tgaagatgac
      721 aatttagctt tagattatga taatgctatt cttgaagttc atggatataa aattatttct
      781 ataacaataa atactttaag tgaatttagt gtagaaggtt atatcaatga tgactgcata
      841 aaatctaaga actcctatgt tgaatatgta gattttaata taataaactt agataaaata
      901 cctggaaaac tcattaaata caatgataag gtatacgcag gtagaattga atttgatata
      961 aatgattatg ttgtaactat agacaagcga tatgattata gaaaagaatt aaaatcagaa
     1021 ttaaagtcta aatctggagc aataataact catataggta ggattagaag aaaagatggt
     1081 agaattttta gaacaaataa tacaataaat cttttagata ggatatcaac agctttgagt
     1141 tttatgtgtg gaagatatgt agggttttgt ttggccaaag ggtatagaag tggtaatgaa
     1201 gtttatagaa tatggaatga aaatcaaata tctcctttta gatatgtacc aacatggtca
     1261 gatacacttt caaattatta taatatggaa aaatatataa gccttatgtg taaaaagcta
     1321 gaagattttt attatggttc tgctataaag agtgttgttg attggtacat agaatctctt
     1381 ggaagtgcta ccatggaaaa caatataata tctgtacaaa ttgcacttga aactttgtca
     1441 tatgtaattc tagtggaaca aaataagata cttacagatg aagtatttga ctgtaatttg
     1501 gcatctaaaa atataagact cttacttgat acatgtaaga taccatatgg taaacatgaa
     1561 ttaaatatat ttgataatat aataaaaaat aaatttgatg atggagtgga tttagttatt
     1621 tatttaagaa atagcatagt tcatccaagt agaaagactc atagagctgt gttagaggtt
     1681 gaagatattt ggaatataat ttcaatagga acgagatata ttgaacttgt tttactcttt
     1741 attttaggtt atagaggaga atattcaaac agattagtag aaagatgtta tggtgaagtg
     1801 gaagttgtac cttggaacta gatttttata tttattttaa aatatatatt gacatatatt
     1861 gccttatgct ataaaatcaa tatcataata ataattcaga ataacattaa atgctaagaa
     1921 aagaaatagt aaaagtagtt aatcttaaac agagagtttc cgattggtga gaggagcaat
     1981 agattttact ttgaacacat ctttgagcag gactctgaaa gagtagaaat actttattag
     2041 gagatttccg gaatccttgc acccgttata gtgctagagt ataataattt tattgtactt
     2101 aaagaggtta gtgtcgtgag gcattaatga acagaggtgg aaccacgaga gttaactccc
     2161 gtcctctagc agaagctaga ggcgggagtt ttttaatata attttttaag cgcgcagaga
     2221 attatattgc aatagataca ctttttaaaa aataatcaaa atattttaaa aattaaaagg
     2281 cgagggagaa tattatgaaa aatatattaa aaaaagtagg aatatttaca attatgttag
     2341 gtttattagg tggagttgta ggttgttcaa aaccagataa tgaaaaagat aaggatgctt
     2401 caaaggaatc taaaaaagaa gtagttgtag ggtttgataa tacgtttgta ccaatgggat
     2461 ttttagatga aaagggagat acagtaggat ttgacgtaga cttagctaaa gaaacattta
     2521 aaagacttgg aatggaagtt aagtttcagc ctatagattg gtcaatgaaa gaaactgaat
     2581 taaatgattc aaaaactgta gatgtattat ggaatggata ttctataaca gacgaaagaa
     2641 agaaaatagt atcatataca gaaccatatt tacaaaataa gcagataata gttactctaa
     2701 gtgactcaaa aataaattca aaagctgatt taaaagataa agaagttgga acacaacaag
     2761 gttcaacagc acttgatgca gtggaaaagg ataaagattt tatgaatagc ttaaaaggtg
     2821 gagcaccagt tctttatgat acttatgata aagcacttag agatttagag ataggtagaa
     2881 ctagtgcagt tgttggtgat gaagttttaa ttagatacta tatgggacaa aagggtgaag
     2941 acaaatacaa ggtattaaaa gacgattttg gattagaaga ttatgttgta gcaacttcta
     3001 aagaaaatcc agaactttgt gagaaaataa atgaaacatt aaaagaaatg aaaaaagatg
     3061 gaacttttga caagatttat gataagtggt ttaaatagaa gataagttag ctgttaagaa
     3121 gggggcacta aaggcatgtt acaaggttta gagatagtta tagcaatgtt ttgtataaca
     3181 ttgatagtat caattccatt ggggattgga gttgcatttt taagattatc aaagaataaa
     3241 ttagtaagtg gtattacaca atgttatata ttgataatga gaggtacacc tttacttttg
     3301 caaatgatag tcatatttta cggattacct cttttaggaa ttgtatttga cagatttaca
     3361 gctggagtag tagctttctt cttaaattat gcagcatatt ttgcagaaat atttagaggt
     3421 ggaatacagt caatcgatag aggacaatat gaagcttcaa aagtactagg atttgataag
     3481 tttactatgt ataagagagt aatctttcca caagttttta aaagaatact agcacctatt
     3541 tcaaatgaag taatcacatt agtaaaagat acatctttag tatatatatt aggattaaat
     3601 gatattttaa gaatatctca aatagcaatg aacagggaag caagtctgtt accattattt
     3661 gaggcaggag cgatatatct tatatttgta gctatactta ctaagggatt tgaattactt
     3721 gaaaaaaaat attcttatta tagataaagt tacaatatta taggaggagg ttttttatat
     3781 gttgaaaatc aaaaacttaa ataaatcatt taaaaaaaat agagtactta aagatatttc
     3841 ttttgagtta gaagaaggtc agattggagt tttacttggt aagtctggtg caggaaagac
     3901 cacaatatta aggtgtataa atggtcttga agaatttgat agtggagaga ttataataga
     3961 taatgaggta ataaaaaata agagagatat ggcaaaaata cgaggaaaaa taggtatggt
     4021 ttttcaaaat ttcaacttat ttcctcatat gacagtcctt gaaaatataa ttgaatcccc
     4081 agttaatgtt tttaaagttc ctagaaaaga ggctgaagag agggctagag aacttttaag
     4141 attagttgat ttagaagata aattaaattc ttatccattt gaattgtcag gaggacagca
     4201 acagagagtt gctatagcta ggtcatgtgc attgatgcca aaagtccttt gttttgatga
     4261 acctacttct gcacttgata ttgatactat tcaaagagta gtcaatataa tgaacagact
     4321 taaagacaaa ggtatgacaa tattaataat tacacatgat gttgtcttct caaataatgt
     4381 tgcagataaa attataagta taaaagatgg tatagtagaa aatgttcaaa taaaagaaaa
     4441 aattgtttag agttttataa atataaacta gggtagaatt ttataatata aataaagggc
     4501 aagtaaaatc aaatatacta tgcccttttg ttatctcaaa atttaat
//
LOCUS       DAMKMI010000213         4540 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMKMI010000213 DAMKMI010000000
VERSION     DAMKMI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.219"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(1008..1418)
                     /locus_tag="PC429_003573"
     CDS             complement(1008..1418)
                     /locus_tag="PC429_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849026.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1634..3016)
                     /locus_tag="PC429_003574"
     CDS             complement(1634..3016)
                     /locus_tag="PC429_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF3849027.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNGKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(3027..3386)
                     /locus_tag="PC429_003575"
     CDS             complement(3027..3386)
                     /locus_tag="PC429_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3849028.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3591..4370)
                     /locus_tag="PC429_003576"
     CDS             complement(3591..4370)
                     /locus_tag="PC429_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF3849029.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1566 a          777 c          401 g         1796 t
ORIGIN      
        1 tattttacga ttactctttc accctaaccc tttaattatc tattttttct gatattctta
       61 tctaccatca tccatatatt tattttacga ttactctttc accctaaccc tttaactatc
      121 tattttttct tactattctt atccacatca tccatatatt tattttacga ttactctttc
      181 actctaaccc tttaactatc tattttttct gatattctta tccaccatca cccatatatt
      241 tattttacaa ttactctttt accctaaccc tttaactatc tatttttttc tggtattctt
      301 atctactatt atctgtatat ttattttatg attactcttt taacctaccc cctatacttt
      361 atagtaatat gtttttaacc tttttcatta ttcccaatat gctttataac tacaaatact
      421 ctcccccctt aaaaataata ataaaattaa tcaataaatt tataatcata ttattactct
      481 atgtattaag caattactat gccaataaac tttgattttt ttaaacaaat cttaaaaatc
      541 ctaaaatgct aattttcttt aacttttaat gaatctaata gataacattt aattttcgtt
      601 atatttttca gaatatttct attattattt ttaagaaaaa aaaataatgt taacatttct
      661 taacatacat taaaaaatgt taacattata aattgggttt aatactttta ctttgactcg
      721 atttactttt attctacttc ttactttatt tttaacttag actttatttt tttattactt
      781 atttgagtct tatttatttt attatcactc catatgttca attacatttg tctaaattct
      841 ttgatacttt tattacataa ttttaacaag cttaagtgtt atttgggtag tgcagttgat
      901 aattatttaa ataatcatca actgcatgtt tatatcatta cagtagctta atgtatttaa
      961 tcatacttac ttaggatttt gaaatatctt agagattata tttaacatta gtcaattgct
     1021 gcataattgt aagcaattga aatttgagac ccatcttcat tttctatagt gataggcttc
     1081 ataattttta atccttcagt ttttatcttt tctaattctt gttccatttt cttaattgtt
     1141 tcctcaaggt ctttttgact tacatctttg actgcattag ggtctttagc atagtctttt
     1201 aattcttgaa tatgtttagc atactcttca taagtataat gctcaacacc atacttttcc
     1261 aattcagtag ggtcagttat tcctgatttt attagagatt caagtttagg taaagcaata
     1321 ttagatgaat catttccatt tgcaaatact gctccagttc ctcctagtac cattgcagat
     1381 accatcattg cacttataag ggattttttt attttcataa ccaatcttcc tttcttagag
     1441 atttatttgg gtagtgtagt ttgaaatgac tattgcaata gttatcaacc acatccttag
     1501 actatcacag tagtcatgtt tatgtcaagc ctttttaaaa aacttttcaa taaaaaatac
     1561 tatctcaaaa caaaattaat tattttgaga tagtatttta attctaaatc ttttatatta
     1621 ctaagattta tggttactct atccatgttc tatattgttc tttattaact gctaacaaat
     1681 tcccttttct tgatatgaca aagcgattat cattatagcc actttgctca attgaaactt
     1741 ctcctgaatc agtataaagt acatccttac cattactaga tttatttaca tacgtttctt
     1801 cgctattctc agaattagtt actttgctca tagatgattc ttcatgctct ctaaattcaa
     1861 ttacagatac ttttgccgta ctaaaatgag tctctccatc acttttagct ttactaacaa
     1921 gaatatcaga ttctactttg ggagaatata aatcagttga tttactactt attgaaaatc
     1981 caattttatc actttgttca ttttttgtta aaccaaaaga ttcaacctca ttaatctctt
     2041 tattttctgt tgcaaaaaca agacctgtcc cacaaactaa tacagttgaa actaaaaaag
     2101 taacataagt tgatttttta aatttcataa tattaactat tctcttttct atttttgtct
     2161 ttccaaatcc actacatatt gtcattgaaa atggattatt ctcctctgct agacttatta
     2221 gtgtggttgc ataatcagcc ctatattctt caccaagtat actcataacc ttttcatcac
     2281 aactaaattc tatgtcttta tttagtaaaa aaaacatcac ccaaactaag ggattaaacc
     2341 aatggacaac aagagctaca agcataaata actttaacag attatcaaag ctattaatat
     2401 gaaccatttc atgagttaaa acaaagtcta aagtttgatt attagcataa ttcatttgct
     2461 ttggcaaaat aattcgtggt tttaaaatac cagatgtaag tggtgtccta gtaaattcag
     2521 atatgcaaac aataaatctt ctaaaacggc tattcttaca catccatttt ttaatgaact
     2581 catgattttt tatatgaatt gaagttttaa aaagattaat tattttaaaa tatctaaagc
     2641 ataaaagtat gctaaacata atagtaacta taaaagacaa aatgcccaac atttccaaca
     2701 tcataatatt ttcaatatta gacattattt caaattcgtc agaattgttt ttacctgtag
     2761 agtcactagc tttcttaaga gtataatttt ctgttgatgt attacccaat tccacatcaa
     2821 aagtcgattc aattatacta taaatactaa aaccagaatt taaacttact ggtattagaa
     2881 gtttaaataa aacaatcaac cacaataata caaatacctt tttaggtaat tttttaaata
     2941 aacatgctct tataaaaata actattaaaa gtaatactcc agaagtaaaa ctcatttcta
     3001 acaatttaat gaacataaat cttacctcac tttaattttt caacaatttg tttaagtttt
     3061 gtaatctcat cacttgaaag attttcttta ttaataaaag ctgaaagaaa tttttctgaa
     3121 gaccctccaa acattttatt aattaaagtt tctgtttcct gtttttgtac ttcctctttg
     3181 cttattaaag ctttacaaac aaaattcggc tcaattctct ctattgcacc ttttgacact
     3241 aattttttta taacggtata cgtagtgttt ctattccatc ctgtttcttc ttttaaaatt
     3301 tttactaact ctccagcctt gctttctcct ctctcccata gaacttccat tactttaagt
     3361 tctgaatcaa ataattttat ctccatataa aatcgctcct caaatatagt tatcacaata
     3421 gtcattacta taataaaaaa ctatcatagt agtctatact atgtcaatca tttatttttt
     3481 aataataaaa actgtctttt taaaaaattc ttataaaaga cagttttatc aatgttatac
     3541 tttaatcttt aattaaagcc atattttatt taaatatata actatatatc ttagtacata
     3601 gtataagatt tttctttatc taatagtata tcctctacat ctgggttttc ttttactaat
     3661 tgtctataaa aattacaatt agtaacacct acatatggaa ttagccatag tagacctatt
     3721 ccaagagtag ctattgaaag tatataccat cctataaaac taaagtttag tacaaataat
     3781 ttaagtacat ttcctttcat catatctaaa ctagcaccca ttgatttaaa tacacccata
     3841 ttatccttgt cttcacaaat taagaaagta gcttgagaaa catataatag caatatataa
     3901 accggtaaca taagaacaag tattataaga actagagtta ttccagtaga aataacagtc
     3961 cctattccaa cattatcact tgataaatat tcaattgctc cactagtaag taaaactcca
     4021 aatgataaaa acgctcctat aattgtgatt ataaaagaat aaatataaac aataattgat
     4081 tcaaataaat aaaaaccaaa acttcttaaa taaattctcc cacttacaaa gatatctcct
     4141 atcttgactt tttcatcttt agtaattttc aagaacatac atgtaaatcc tgctgaaacg
     4201 caaaaagcta ttacagccac tacaatagta gtcaatggtt caaaaggtaa tcctacaatt
     4261 gatattaata ctatcaatgc aatatatact gccatagaaa ttgcacatat cccccagttt
     4321 ccagataact gagctttaga tactcgtttt aattcttgcc ttgataccat aaaaaacacc
     4381 cctttcattt tataaaaaaa cttttaaaaa tataaataat cttttaacgt ataatattaa
     4441 tatatcatat tttcgactat ataagataca attattcacc atgttttatc aagatatacc
     4501 ataattgtat tactagtttt atatagtcaa cattaccaaa
//
LOCUS       DAMKMI010000214         4443 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMKMI010000214 DAMKMI010000000
VERSION     DAMKMI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.220"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            152..1627
                     /locus_tag="PC429_003577"
     CDS             152..1627
                     /locus_tag="PC429_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF3849030.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1714..2127
                     /gene="speD"
                     /locus_tag="PC429_003578"
     CDS             1714..2127
                     /gene="speD"
                     /locus_tag="PC429_003578"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF3849031.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2157..3008
                     /gene="speE"
                     /locus_tag="PC429_003579"
     CDS             2157..3008
                     /gene="speE"
                     /locus_tag="PC429_003579"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF3849032.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            2998..3876
                     /gene="speB"
                     /locus_tag="PC429_003580"
     CDS             2998..3876
                     /gene="speB"
                     /locus_tag="PC429_003580"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF3849033.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4042..4242)
                     /locus_tag="PC429_003581"
     CDS             complement(4042..4242)
                     /locus_tag="PC429_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF3849034.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1755 a          495 c          764 g         1429 t
ORIGIN      
        1 tttgagtaac ttcaaaaaaa cacttgtaaa aatgctggga tgttaatata ataaatatca
       61 tgagtttaat aaaagtaaaa ataaaaaata gaaataataa ataataaata ttaaaaatat
      121 ttgaatatag agaggagatg aatttgaaat catggatagc gagtttgcaa atgcagttga
      181 attaagctat gagtgtgctc cattattaga gtcgttaaag gaatactcag agaaagatat
      241 agcttgtttt gatgtgcctg gacatgtaaa aaatagaggt gtagctatat taaataaata
      301 tctaggtgaa agtctcatga aaatggatat aaattcatca ccaactatgg ataatgtatc
      361 tgcgccaaat gggattataa aaaatgcaca agatttgtta gcacaagcat atatggctga
      421 tgaggcattc tttataacga atggcacaac tcaagcaata catgcaatga ttttgagtgt
      481 tataaaacca ggtgaaaaag tattacttcc aagaaacata cataaatcag taataaatgc
      541 gttaatactt tgtggaggaa taccaatatt tatacaacca gaatttgatg aaaaactagg
      601 tataagttta aatattactc ttgaaaaagt tagaacagag atagaaaaag attgtaatat
      661 aaaagcatta tttttcctta atccaactta ctatggagtt tgtgcagacc ttgagagtat
      721 tatagaacta tgccataaaa ataatgtttt agtgttagtt gatgaagctc atggtgcaca
      781 ttttccattt catttagatt tacctccttc tgcaataagt ttgggagctg acatggtagc
      841 tgtaagtata cataaaactg gaggagcatt gacacaatct tcagcactct tattgaatag
      901 agataatgta agttttgaaa aggttcttca atcaatcaat atgctacaat caacatcagc
      961 atcttatttg cttatggcaa gtatagatgg tgctagagtt aatctagttg agaatggtga
     1021 aaagcaattg tcaaaagcat taaatctatc taggtatgca aaatctaaat tgaacaaaat
     1081 tgatggaata aaagttttat ctacagaaat attgaaacaa aaaggtgtta aatttataga
     1141 tgaaacaaaa ttgtgtataa atgtaaaaga attaaatttg actggatttg aagtatacga
     1201 tttattatat aaaaattttt caattcaagt agaattggga gattcatata atatactggc
     1261 acttgtgtcc attggaacaa ataaatcaga tatagataga ttagtaaagg cactttctat
     1321 aattgcaaag atttatagaa aagaatcaac attaaatgaa tttaatatgg ttcaaataaa
     1381 tccaattatt aaacttaatc caagagaagc tttttatgca ccaaaggaaa gtgtagaaat
     1441 caattcatgt atagatagaa tttgtggaga atcaataatg gcatatccac caggtatacc
     1501 gattattgct ccaggagaat tgataacaga agaaattatg gagtatatta ttttcttaaa
     1561 aaatagcaat gcatatctta cagatgtaca agataaaaac ttagatagaa tactagtaat
     1621 aaaataaatt actttatttt gcaagggcaa gtgtttgtta agtagagttt atcaaataga
     1681 acttattaaa agtataaagg agttggggtt gaaatgaaat ttgaaacact aggaagacat
     1741 atactagtag aatactataa ttgtgatgaa aacgtactta aagacccacg tcttatagaa
     1801 aattttatga atgattcagc attaaatgca aaagcgacaa ttgtggattc tgtattccac
     1861 cattttaatc cttggggggt ttcaggagct gttataattg ctgaatctca tttgacaata
     1921 catacatggc ctgaatatgg ttatgcagca gcagattttt ttacttgtgg tgatatagac
     1981 ccatggaaaa gttttgaatt attagagcaa cttttaaagt ctgaaagaag cgagtctaca
     2041 gaaattccaa gaggcttaac tacaaaaatt aaaaaatatt caaaaaaaga tttaggtaag
     2101 attactcata aaccagaagc agtataaaac atacaagaga aagtgaggat tattatatgg
     2161 aactttggta tacagaagaa tggacagaaa atgtaagatt ttctataaaa gtaaataaac
     2221 atctatttga aggaaagtct caatttcaaa ggatagatgt gtttgatagt gatgaatttg
     2281 gaaaatttct aacaatagat ggattaatga tggtaacata taaagatgaa tttatatacc
     2341 atgagatgat aactcatgtt cctatggcta caaatttaaa cataaagaaa gtattagtca
     2401 taggtggagg tgatggaggt acagtaagag aattaagccg ttatcctcaa atagaaaaga
     2461 ttgacatggt tgaaatagat aaaatggttg tagatgtatc taaagagtat atggatatat
     2521 gctcatgtaa attagatgat aaaagagtga gtttatattt tgaagatggt gttaattttg
     2581 ttaagtgtgc acatgataaa tcatatgacc tgataatagt agattcaact gacccaatag
     2641 gaccaggtga agggcttttc tcaactgatt tttataagga ttgttataga atactgactg
     2701 atgatggaat tttagtaaat caaagtgaaa gtccgtattt tgactttaat gctaaagaga
     2761 tgaaaagagc taataaaaag ttaaaacaga tatttccaat atcagaagtt tatcaagcac
     2821 atataccaac atatccatct ggtcactggt tgtttggatt tgcttcaaaa aaacttaatc
     2881 cagtaaaaaa tcaagataga aatggatggg agaaattaag tttaaaaact aaatattata
     2941 atagtgatat acatttaggt tcgtttatgt tacctcaata cgtgaaggag atgctggatg
     3001 aagaataatt tctatcatat gaacactttt atgagtatga acaaaaatta tgaagaatct
     3061 aatcttatag tatttggtgt tggatttgat ggaactactt ctaacagacc tggggctaga
     3121 tttgcaagta gctctatgag aaaagaattt tacggtcttg agacatatag tcctttttta
     3181 gatttggatt tagaggatta taatatatgt gattatggag atttagaaat tagtgttgga
     3241 agtacagaac aagtcttaaa agaaatctat caagagacat ataagattgt tagagattca
     3301 aaggtaccct ttatgattgg aggagagcat ttagttacat taccagcctt taaagcagta
     3361 catgaaaagt ataatgatat atatgtaatt cattttgatg cccatactga tttgagggaa
     3421 gaatataata atagtaaaaa ttctcatgca acagtaatta aaagaatatg ggatattgta
     3481 ggtgataata aaatatttca atttggtata aggtctggga caaaagaaga atttaaattt
     3541 gctacagaag aaaaacacac atacatggaa ataggaggaa tagatacgtt tgaaaatata
     3601 gttaatatgc taaatggaaa gaatatttat ctaactatag atttagatgt attggatgca
     3661 tctgtgtttc caggaacagg tacaccagaa cctggtggtg taaattatag agagtttcaa
     3721 gagattttta agattataaa aaactctaat ataaatatag ttggttgtga cattgtagag
     3781 ttaagccctg attacgatac aacaggtgta tcgacagtta tagcttgtaa aatcttaaga
     3841 gagttatgct taataatatc tgataaaatt aaatagagta ttttatataa atagtatata
     3901 ttataatata agaagttatt ttaagtttga cgcttttcta aagtaacttc ttatttttgt
     3961 aattaaaaat aaatataata aacaacaaaa aagagccctc gatagctcct tttctaaaat
     4021 atatatattt aaattaaata attatattct atttacgttt tcagcttgag gtcctctatt
     4081 accttgagtt atatcaaagc taaccttttc gccttcttct aaagttttgt atccatcatt
     4141 ttgtatagct gagaaatgta cgaaaacatc atcttctccc tctacagata tgaatccaaa
     4201 tcctttttca ttgttaaacc attttactat tccgtttttc attataagaa aacctccgca
     4261 ttattaaaaa atacaaacta aaaaaataag tctgttctat taatataaca taaattttaa
     4321 ataaaagcaa ataaaatttt taaaaaaatt aattactttt agaatttaat aaaaattgtt
     4381 aaattataca gtgagaagta ttgtaaaatt tgacgaaaat gtaaatgtag ataataaatt
     4441 taa
//
LOCUS       DAMKMI010000215         4442 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMKMI010000215 DAMKMI010000000
VERSION     DAMKMI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.221"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            149..1747
                     /locus_tag="PC429_003582"
     CDS             149..1747
                     /locus_tag="PC429_003582"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF3849035.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1982..4063)
                     /gene="bclA1"
                     /locus_tag="PC429_003583"
     CDS             complement(1982..4063)
                     /gene="bclA1"
                     /locus_tag="PC429_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF3849036.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGVTGATGPTGATGVTGEIGPTGATGPTGEIGPTGATGPTGATG
                     VTGEIGPTGVTGPTGEIGPTGATGPTGVTGSIGPTGATGVTGEIGPTGPTGVTGSIGP
                     TGATGPTGNTGVTGVTGPTGATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGPT
                     GNTGATGSIGPTGVTGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNN
                     GDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFP
                     ILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATL
                     TIFRIADTVMT"
BASE COUNT         1311 a          789 c          683 g         1659 t
ORIGIN      
        1 attaaatttg tagtatttat gtaagaaata aattttatat aatatttttt cactaaattt
       61 ttttcttttt acaacaaata ctcactacct tttgatataa tatttttatc aattattatt
      121 atattcaagt tttgaaagga gtattaaaat gagtttatta gttttcggac ataaaaatcc
      181 agatacagat tcaatatgtt ctgcaatttc attagcacat ttaaagaaca aactaggaat
      241 aaaagcaaca gcttatgctt taggagatat cagaaaagaa gctaaatatg ccttggatta
      301 ctttaaggta gatgcacctg aaattttaga taatgttaga atacaagtta gagatttaaa
      361 ttatgataaa gtagtacctt taacaccaac ttcatctata ttagaggctt ataaccttat
      421 ggatgaaaaa aatgttaaaa cactacctgt aacttatgat gatggaactt ttgctggtat
      481 cattactatg aaagaaatag ctaaaaatct acttcatcaa cactttacaa ctatacacac
      541 atcactatca aacatttgta aaaaccttaa tggtactatt cttgtggact gtggtgaaga
      601 tataaaaggt agagtagtca ctttatcatt tggaatggag actctaatag agactttaaa
      661 agaaggagac atagctattg ttggagatag atatgatagt attgaatatg ctattgatac
      721 aaaagttaaa cttttaatac ttactaatca tacagagatt tcaaaagatt tgctagctct
      781 tgctaagata aatggagtgt ctgttgtttc tgttgaaagt gacaattata aaacttctaa
      841 tgtaataaat caatgtagtt tcttagactc tatagaagct acagaaaatt taattacatt
      901 caaagaagat gattatatgg atgaaataaa agagattatg ctagaaacaa acttcagatc
      961 ttatccaata ttagatgatg ataataagtt tttaggttta gtttcaaaag gtcatctatt
     1021 aaacccatct aaaaagaatg ttgttttagt tgaccataac gaatatgctc aaagtgctga
     1081 tggaattgaa caagcaaaca tagttgaaat aattgaccat cataaacttg gcggtatttc
     1141 tactgatgtt cctatgtcat ttagagttat gcctgttgga tgcaatagta ctataattta
     1201 tcaaatgtac aaagaaaata atgttgagat accttatgaa atagctgggc ttcttttatc
     1261 tgctatatta tcagatacat tattatttaa gtctccaaca acaactgata tggataagaa
     1321 agcttgtgaa gaattaagta aaatagcaaa agtagacatg gaaaaatacg ctatggatat
     1381 gtttaaatgc ggtacttctt tagatgaata tactatagaa gaaatagtaa atatggactt
     1441 taaagaattt aatatgagtg gacatagagt tggtattgga caagtattta ccttagacat
     1501 agactctata tttgctaaaa aagatgagtt tttatcttat ataaattcga ctgattatga
     1561 taaattaatt ctttcagtta cagatataat caaagaaggt tcttatttaa tctataaagc
     1621 agaagatagc ttaattgcta acgcatttgg tgttgaaggt gttcaaggca catttgcacc
     1681 aggtgttgta tcaagaaaaa aacaattagt ccctaattta actactgcaa tcaaaaattt
     1741 taaataatat aaaattttta aataacgaaa actgtctcaa atctatattt gagacagttt
     1801 tttattatat atatattgat aataaagtta aataaaaatc cataaattta aaatttttat
     1861 gaatatattt tattattatt gttattttat tcattctaaa ataggtaaaa ctcaatatat
     1921 aaaaatactt ttgtcattta aataaaacct aaatagtttt taagattatt ttagacacgt
     1981 tttaagtcat aacagtatca gctattctaa atatcgtcaa agtagccgca gttcctgctg
     2041 attgcccatt tcgtatccct actgttgtag atgaaaagtt atttattgta aaagtagtcc
     2101 caccaaataa aaaaccagca aaatatccag aaagtactcc tccgcctcct ggctcagtag
     2161 taagccctgc caaatctttt cctcctaaaa ttgggaatag gatagcgaaa gaatattgat
     2221 ttatacttga tgaacctggt tgtaatgcta ctgaaactga tatataatac acaccatttt
     2281 cttgtactgt aaacgtacca tttgcatcta ctgttatact tgaactattt cctattattg
     2341 tttgccaacc tgttatagca tctccattat ttagtgtttg tggagatggt gcatttacga
     2401 ggaagttagc aactggctga gaggaattgc ctgttgctcc tgttggacct gttgggcctg
     2461 ttactcctgt cgctcctgtt gctcctgttg gacctgttac tcccgttggc cctatacttc
     2521 ctgtcgctcc tgtatttcct gttgggcctg ttggtcctgt tgctcccgtt ggtcctattt
     2581 ctcctgttac tcctgttggg cctgttgctc ccgttggtcc tatttctcct gtcactcctg
     2641 ttgggcctgt tgctcccgtt gggcctgtta ctcctgtcac tcctgtattt cctgtcggtc
     2701 ctgttgctcc cgttggtcct atacttcctg tcactcctgt tgggcctgtt ggtcctattt
     2761 ctcctgtcac tcctgtcgct cctgttggtc ctatacttcc tgtcactcct gttggtcctg
     2821 ttgctcctgt tggtcctatt tctcctgttg ggcctgttac tcctgttggc cctatttctc
     2881 ctgtcactcc tgtcgctcct gttgggcctg ttgctcccgt tggtcctatt tctcctgttg
     2941 ggcctgttgc tcctgttggc cctatttctc ctgtcactcc tgtcgctcct gttggacctg
     3001 ttgctcctgt tactcctgtt actcctgtat ttcctgttgg acctgttgct cctgttactc
     3061 ctgtatttcc tgttggacct gttgctcccg ttggtcctat ttctcctgtc actcctgtcg
     3121 ctcctgttgg acctgttgct cctgttggtc ctatacttcc tgtcactcct gttgggcctg
     3181 ttactcccgt tgggcctatt tctcctgtca ctcctgttgg gcctgttgct cccgttgggc
     3241 ctatacttcc tgtcactccc gtatttcctg tcggacctgt tactcctgtt ggtcctatac
     3301 ttcctgtcgc tcctgtactt ccggttgggc ctgttactcc cgttggccct atacttcctg
     3361 ttgctcctgt atttccggtt gggcctgttg gtcctatgct tcctgtcgct cctgtacttc
     3421 cggttgggcc tgttactcct gttgggcctg tacttcctgt cgctcctctt ggtcccattg
     3481 gacctgtaat tctaattgga agacactcac atttaggata acaattatct ataattctat
     3541 cttcaaatgt tacaagtata tacggtttgt tacaacctct actagatgta aattcaagaa
     3601 tctgataagg gtaatttaaa gataaagcta acccataatt aggatattta ttcattgccc
     3661 atgcttttat agttcccgta atatttaaac aaatatagtt ggatgtatca cttatcccaa
     3721 ttggtaaata tcccctgata ttttccatag acactctatc attccatgtt atagttcgta
     3781 aattataata ttcacttatt cttttaattt caatatctac tttttcttta aaaatatgat
     3841 tcttatttga atcaatatga ataaataact ctgcttttaa tatacttttg tcttttaata
     3901 tatctggtat tctcacttta aaaaaagtaa tcaacttttc ttttacaaaa ctatttgaac
     3961 atttacttcc tattaagcaa taatctaaat tactaaagtt tttatctgga tattttttag
     4021 atataaaagt atcatcattt aaataaagta taatatttct catttataat ccctccattt
     4081 tgggtctata tcatactatg tttaaaatac atgcttagtt acaatacaat taaaatttat
     4141 agtattaaat ttttatataa taatgctgat ttctacaaaa aaagaacatg tcattagaaa
     4201 tttctaatag catgttcttt ttcattatta tatattacac tttaaattat tccttgactg
     4261 gcttagctgg tgtaaactaa accagtcttt ctaaaatatt aagcactttt ttcttattat
     4321 gttttttagg atttattgtc tatattattg taaaccaaag ttataaaatt gtaaatagaa
     4381 tatattacca taacaatatt tttttataag taaaaattat aaaaaacttt atttttgtta
     4441 aa
//
LOCUS       DAMKMI010000216         4289 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMKMI010000216 DAMKMI010000000
VERSION     DAMKMI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.222"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            231..1310
                     /locus_tag="PC429_003584"
     CDS             231..1310
                     /locus_tag="PC429_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF3849037.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC429_003585"
     CDS             1634..2002
                     /locus_tag="PC429_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF3849038.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDNDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="PC429_003586"
     CDS             2119..2922
                     /locus_tag="PC429_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3849039.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="PC429_003587"
     CDS             3307..4197
                     /locus_tag="PC429_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3849040.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
BASE COUNT         1642 a          470 c          803 g         1374 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagaacgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg aaatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggataa tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtttttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaacaa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaattaca tactcattag
     2941 gtaaattatg tagtatatgt gtttatagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggacagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagagat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaggcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat tgactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
     3961 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtatttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tgttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gatattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtatt
//
LOCUS       DAMKMI010000217         4156 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMKMI010000217 DAMKMI010000000
VERSION     DAMKMI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.223"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            215..2314
                     /gene="nrdE"
                     /locus_tag="PC429_003588"
     CDS             215..2314
                     /gene="nrdE"
                     /locus_tag="PC429_003588"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF3849041.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            2326..3297
                     /gene="nrdF"
                     /locus_tag="PC429_003589"
     CDS             2326..3297
                     /gene="nrdF"
                     /locus_tag="PC429_003589"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF3849042.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            3373..3999
                     /locus_tag="PC429_003590"
     CDS             3373..3999
                     /locus_tag="PC429_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF3849043.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
BASE COUNT         1661 a          449 c          774 g         1272 t
ORIGIN      
        1 aagtttagat ataaaaaaat ttgtgtatat actatatgta gtatataata gataaagcac
       61 tgactatatg tagtgctttt gtttttgaaa aatacttatt gcatttctat aattatagat
      121 taattttggg atttcaactg ataagtacca aaagaaataa taagcaaatt aagtgaaatt
      181 atggcacaag attatgttaa aaggacggta aataatgaat tggatagagc ttaacaatca
      241 agtgataatt aaaaatgaac aaggcaagta ccaattggaa aaggataagg aagccttaag
      301 tagttacata gaagaattta tacagccaaa gttgagaaaa tttaataacc tagaagaaag
      361 attgaagtat ttaatagatg aaggttatta ttcaaaagaa gtaataaatc agtatagcat
      421 agatgttatt aaaaatatat atgatatgat tgataaacat aagtttgaat ttcaatcata
      481 tatgagtgca aataagtttt atcaaaatta tgcattaaag agcaatgatg gaaaggaaat
      541 attagaaaca tataatgata aagtgttaat tgtagctttg acactaggaa atggtgataa
      601 agatttagct ttaaacttgg cagataaaat agtaaaacaa gaatttcaac cagctacacc
      661 tactttttta aatgctggaa gaaaaagggc tggagaaatg gtatcatgtt ttttactatc
      721 tgtagaagat agtacagaag gtatatcata tgctatatct tcatcaaatc acttatcaaa
      781 gattggtgga ggagttgcac taaatttatc taaacttaga gcatctggcg aatctatcaa
      841 agatatagaa ggtgcagctg gaggtgttgt tggagtagct aaaatgttag aacaatcatt
      901 tagttacttt aatcaaatgg gagctagaca aggttctggt gcggtgtacc ttactgtatt
      961 ccatcctgat tttgaattgt tgatggatac taaaaagata aatgcagatg aaaaaataag
     1021 gcttgctaca ttatcattag gagctataat accagataaa tttatggagt tggcagaaaa
     1081 aaatgaagta gcatatgcat tttatcctca tactgtgtat aagaaatatg gagtttctct
     1141 tgatgagata gaaatggatg agtggtatga taaattggtt aatgatagtg atataagaaa
     1201 aaaagaaata aatcctaggc agatgttgac aaaaatagca cagatgcaac aagaaagtgg
     1261 atatccatat gtagtgtaca tagatacagc taatagagaa catacattaa aagatgttgg
     1321 aattattaaa atgtctaact tatgttgtga gatatttcaa tatcaaacac cttcagaaat
     1381 agaggggtat ggaggaaaaa atgaatgggg acaagatata agttgtaacc taggttcttt
     1441 aaacatagct aatgtaatgg ataataaaac tatagaaagt acagttgaaa cagcaataag
     1501 agcactgtca tttgtagcag atagcacaga tataaaacca gtgccaacgg taagcaatag
     1561 taacagcaag tctcattcta taggattagg agctatgaat ttacatggat acttagtaag
     1621 agaaaatatt ttatatactt cagatgatgc tatagatttt tctaatgtat tttttgcaat
     1681 ggttagatac tattcaatta aagcttctat gaaaatagct atagaaagaa atcaaacttt
     1741 tgaaggattt gataaatcag aatatgttaa gggaagaaat agcaaggttt tatcaaaata
     1801 ctatgaacaa tcttatctac ctaagtcaga aaaggtaaga gcattatttg aagggattta
     1861 tataccaaca aaagaagact ggacaaaatt attggatgag gtaaaagaaa aagggatata
     1921 taatgcatat ttaatggcag tagcaccaac tcaaagcatt agttatgttc aaaatgctac
     1981 atcaagtata atgccaatta ctgagccagt agaagtaaga acatatggag actctactac
     2041 aatatatcca atgccatttt taacaaatga taatatgttg tattatcaat cagcatacag
     2101 gatggatatg agaaaggtca tagatttagt ggcaacagtt caaaaccatg ttgaccaagg
     2161 tatatcaaca acactgtttg ttacagatga aaagacaact agagatatag ctagacatta
     2221 catatatgcc tataaaaaag gtttgaaaag tttatattac acaagaacaa agatgacaag
     2281 agatactcat gagtgtctag tgtgttcagt atagggagag aaattatgac atttaattta
     2341 cataaaatac ataatgcagt taactggaat agagaagaag atggatttac acaagctttt
     2401 tgggaacaaa atgtaaaaca attttggctt cctgaagaaa tatctgtttc taaagacata
     2461 aaggtatgga atgaactaag taataaagaa aaggaattat ataaaaaagt attgggtggt
     2521 ttaacattac tggatacaaa acaggggaat aatggaatac catctatgat gagtttaaca
     2581 gaaaacctac aaagaaaagc tgtcttgtct tttatgggga ctatggaaga aatacatgca
     2641 aaaagttatt cttctatatt tatgactcta ctttctaatt tagaaataga tgaattattt
     2701 gaatggatag aaacagaacc aacattacaa agaaaggctg atttagtttt agctcaatac
     2761 gaaaatacta caaatcaaga gggattatac ctatcaatgg ttacaagtgt atttttagag
     2821 agtttcttgt tttattcagg attcttttat ccattgtatc tatctggtca aggaaagatg
     2881 gtagctagtg gagaaataat atcgcttatt cttagagatg agtctttaca tggtaagtat
     2941 ataggattgc tagcacaaga aatatacgat tcatttgata aaatggataa ggagatgtta
     3001 gaagagaaga tgtattctat actttatagc cttatggaga atgagataga gtatacaaat
     3061 gttatataca gagaaagtgg gcttgaaaaa gaggttgtga actttttaaa atacaatgca
     3121 aatagagctt tagaaaatct agggtttgag aagctatata ctgtagatgc tataaatcca
     3181 attgtattaa atggtcttag cactgaaacc aaaacacatg attttttttc aactaagggt
     3241 aatggatacc aaaaaggagt gtatgaggaa ttagaggatg aagattttat aatataattt
     3301 ataattatga cacactgttg tcacttttag cgtgataata tcaatgtata aaatatatat
     3361 ggaggtattt ttatgaaata tgaatggaga aaaagagata aagagttata tttacctaaa
     3421 aagaatcctg taatagttga aataccagaa cagaaattta tcatgttaaa agggagtgga
     3481 gacccaaatt ctaaagaatt tactgaagca ataggagtac tttattcttt ggcatatgca
     3541 ataaaaacca tgcctaaaaa aggaattgta ccagaagggt attttgacta tacggttttt
     3601 cctttagaag gtatatggga taagggagaa aaaagtaaag gcttagatgt acttattaaa
     3661 gaagatttaa tttacactat aatgatacgt cagccagatt ttgttacaca agacctgttg
     3721 gataaagcta ctgatatagt aaagaagaaa aaacctcata atcttcttga aaaagttaga
     3781 tttgggtcta ttaatgaagg gctatgtgtt caaatgcttc atataggttc atatgataat
     3841 gaaccagaaa gctttgacat tatgaacgaa ttttgcaaaa aaaataaact tataagaaaa
     3901 tcttacacac atcgtgaaat ttatataagt gatgctagaa aagtatcgcc tgaatcattg
     3961 aagacggttt tacgttttga agtagaaagg gaagtataaa tattttgaat aatttataag
     4021 agttataaaa agtgaagtct gttgagactt cattttttga tgcaatttta aaattaaatt
     4081 tgatgtttca taatatttta aaaactttct acttttatta gtgtgaataa ctgttgctat
     4141 taaataataa tactaa
//
LOCUS       DAMKMI010000218         4113 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMKMI010000218 DAMKMI010000000
VERSION     DAMKMI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.224"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            3..401
                     /locus_tag="PC429_003591"
     CDS             3..401
                     /locus_tag="PC429_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3849044.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIK
                     INLNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            389..1327
                     /locus_tag="PC429_003592"
     CDS             389..1327
                     /locus_tag="PC429_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF3849045.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1711..1935)
                     /gene="secG"
                     /locus_tag="PC429_003593"
     CDS             complement(1711..1935)
                     /gene="secG"
                     /locus_tag="PC429_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF3849046.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2528..3820)
                     /gene="eno"
                     /locus_tag="PC429_003594"
     CDS             complement(2528..3820)
                     /gene="eno"
                     /locus_tag="PC429_003594"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF3849047.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1421 a          660 c          459 g         1573 t
ORIGIN      
        1 ccatgataaa aaaaatagaa catgtggtca ttactaataa taatatagaa aattatatca
       61 atttttataa aaaatttggt ttttcatcta aagaaatgag caaaaattat aaacttaatg
      121 ctagaaatat taagataaat cttaacataa aaggttataa atttttacct tatgttaaaa
      181 atttacaagc aagtagagat aacttatgtt ttaaaataaa cacaaatatt gatgagccaa
      241 aaaaatactt agaacaaaat gagattcaaa ttaagcgagg aattgtagaa tgtacaggtg
      301 tctttggtaa tatgaaatct atttatttga aaaaaactaa tggaaattta attgaattat
      361 caaatcatag aatcacggag agtaaacctt ggaaaattta atcctatcaa taaatgttgt
      421 tcttcctttg tttctcacaa tgtcacttgg atatgttcta aaaaaattag gtctatttga
      481 tactcatact ttaaataaaa tgaataatgt ctgcttcaag tctttcttgc cattattatt
      541 attttttaat atatacaata cagatttaga tggttctctt aattttaggc ttatggctgt
      601 agctgttata attatttttt caatcttctt aatcttagtc tttattattc ctaaaattga
      661 aaaagataat aagacaaggg gtgcattaat acaaggtgta tttcgtagca atttcgttat
      721 atttggtatt cctgtgacaa tttcactatt tggcgataag agtattggag tcacatcttt
      781 attaattgca gttattgtac ctatgtttaa tgtattatct gttgtaattc ttgaaatttt
      841 tagaggtggc aaagtaaata taaaacatat gattaaaagc gtcttaacaa atccactcat
      901 aatcgcctct tttataggat tgacaatgct aattttaaat attaatatac ctacattttt
      961 acacaaaact attggtgata tttctaaaat agctactcct ttatcactta taattctagg
     1021 tggctctttt agtttttctg ctataaaggg gcatataaac cccattatat tgggcgtatg
     1081 tggaaaactt atacttgtac ctgctatatt tttaccaatt agcatttttc ttggatttag
     1141 aaatgtagaa ttagcttcac ttatgataat gttatctgct ccaactgcag tttcttcatt
     1201 tacaatggca caacagatgg attctgacag tgaattagct ggacaaattg tagtatttac
     1261 ttctggattt tctataatta cagtattttt aataatattt attttaaagc agaataatct
     1321 tatttaatga tatttgataa cacaaattgg tattatgaga aacatatata aaatcgattt
     1381 aattgcgtaa aaacatatta ttatactatt tttactatat aatataactt tgtgtatatt
     1441 atctcattaa acatggtgaa agtagtgttg ctatatattt tttagtttga aaaaaatata
     1501 taaatacaaa caatatataa atacaaataa taaacgccaa caaatagtca atctataaaa
     1561 taaaaactgg actaatcata taaaatcatt agtccagctt ttattttacg tactttttat
     1621 gttgatgtac ttttattaaa aactaaattt aatttttaaa tgtctagcaa atgcactaat
     1681 taaataatgg ccagttattc aacaatatca ctacttctta actataagta atgccaatgc
     1741 atttataaaa aataatactg ctgttatctt tgtaactcta gcaagaaatg cttgttttcc
     1801 ttttggttta aagtaagctt ggcttccttc tccaccaaat tctgaaggta aagcattctt
     1861 agaatcttga ggcattatgc taattactag taaaatccct agtactactt gcacacccat
     1921 taaaatgttg ctcattcttg cccctcctat ctgtttatta cttaaatttc attactcttt
     1981 agtgtatcac aataatatag ccatttcaag actattatgc tataaataac atacaaaaaa
     2041 atatattgtt actataatat attactatat tctcaatata aaaatgaata aaacttagaa
     2101 gagaaaacta tttaaattaa tgcataaatt aacacttaaa ataatctact ctcattgtat
     2161 atattataaa tagccaccaa aataaaacaa tcattttaaa atattgatat aacttttaat
     2221 ttcaacataa tttaaaataa ataatccaca tttagcaata atactttaat gacaattatg
     2281 attaaatcaa aatatgtctt cacttttatt ttttaataaa aacctcctat gcaattagat
     2341 aaaaatgaga tagaaacatt tattcatatt ttttctgaat ctatgaataa aattaagtaa
     2401 taaaatataa aaaaggataa gtgaattgaa aaatcaatta acacttatct cttttatatc
     2461 ttataagatg agcttaaata tcttatattc tctcttcatt aatctttttt agtaaccatt
     2521 ttaataacta tttttttaag ttatagaaag attttaatcc acaatatcta gcttgctctc
     2581 caaccatttc ttctattctt aataattggt tgtacttagc aactctatca gttcttgatg
     2641 gagcaccagt ttttatttga ccagcattta ctgcaacagc taaatctgct atagtagaat
     2701 cttcagtttc tcctgatctg tgagatataa cagcagtata tcctgctctc ttagccattt
     2761 ctatagcatc taaagtttca gttattgtac ctatttgatt aactttaact aatatagagt
     2821 tagctactcc attttcaatt cctttttcta atctctctgt gttagtaaca aataagtcgt
     2881 caccaactaa ttgtaattta tttcctaatt tttcagttaa taatttccat ccatcccaat
     2941 cttcttcgtc taaaccatct tctatagtta ttattgggaa gttgtttgac caatcttcat
     3001 ataaagcaac catttcagca gcagttaact cttttccttc tcctgctaaa acgtattttt
     3061 tagtttcttt gttgtacatt tctgtagcag caacgtcaag acctaacata acgtcttcac
     3121 ctggcttata tccagctttt gttatagctt caactattaa ttctaaagct tctctgtttg
     3181 aacctaagtt tggagcaaat ccaccttcgt cacctacacc acaagctaat cctttttctc
     3241 ctaaaacttt ctttaacgaa tggaatactt ctgctcccat tcttaaacct tctttgaaac
     3301 aacaagctcc tactggtaat atcataaatt cttgaacatc aacgttgtta tcagcatgct
     3361 ctccaccgtt taatatgttc atcattggaa caggtaattg tttagcattt actccaccta
     3421 ggtattggaa taatggtaaa cctatttcat cagcagctgc tctagctact gccattgaaa
     3481 cacctaatat agcattagct cctaatttac ctttgtttgg agttccatct aattctatca
     3541 tgattgcatc tatagctggt tggtcaaatg catccattcc ttctatttca ggtgctatta
     3601 tttcatttac gttagcaaca gctgtttcta caccttttcc taagtatctt cccttatctc
     3661 catctcttaa ttcaacagct tcaaaagctc ctgtagatgc tcctgatgga actattgctc
     3721 ttcccattgc tccatcttct aatactactt caacttctac agttggattt cctcttgagt
     3781 ctaatacttc tctagcatat actaattcaa taactgacat ttgtatatct cccttcgatt
     3841 ttctattagt taaattttca tttactgtaa acatccgtct actattttaa tctactttct
     3901 atattatagt acataaagtt gcttttgaaa attaggtaca aatttatttg atgggtaaat
     3961 ttgtaaaagt tcctattttt attttatttt ttataatatt ttaatttata taatataaaa
     4021 aaacttgtct aaaatccaaa accaatctta aatacacaaa tattataact agatttaaaa
     4081 cattaaacat aaaatgtaca actaatgatt cta
//
LOCUS       DAMKMI010000219         4109 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMKMI010000219 DAMKMI010000000
VERSION     DAMKMI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.225"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            347..1621
                     /locus_tag="PC429_003595"
     CDS             347..1621
                     /locus_tag="PC429_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF3849048.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVVD
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENILKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1828..2145)
                     /gene="trxA"
                     /locus_tag="PC429_003596"
     CDS             complement(1828..2145)
                     /gene="trxA"
                     /locus_tag="PC429_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF3849049.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2412..3164
                     /locus_tag="PC429_003597"
     CDS             2412..3164
                     /locus_tag="PC429_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF3849050.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3151..4032
                     /locus_tag="PC429_003598"
     CDS             3151..4032
                     /locus_tag="PC429_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF3849051.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTILSGEIMY"
BASE COUNT         1615 a          533 c          568 g         1393 t
ORIGIN      
        1 ttctttacta tttttacaac tttattagtc atattcataa ttatttctaa ttagtgttta
       61 acattttatg taaaatttat aaaaaaatgt atataaatta aaataagtga ctatactaaa
      121 aatatgatac ggataaacaa taagttcaac ctatctcccc caaaatcaag tgagctacag
      181 tttcccccaa ctgtagctca ttttcgtgta atttaataaa catctcaaca agaattaatt
      241 aacaaaaccc cttataaaaa cataacatat attaaacaag ctcttgatta aaacttgagc
      301 ttttttaata aaaaacagaa tagtttatta gataaggggt ttttttatgc aattcatgga
      361 tgtatctaaa tacattgaag gtgcaaacct taaagttaaa actaaaaatg gtgaaactgt
      421 taaaagaata tattttaaca actctgccac tccattagtt ttaaaaaacg tagtggataa
      481 cttaaactgt gagattcctt ggcttactta tattaatgct ccaggaataa tctcagaaaa
      541 aaatacttta aaatatgaaa atgtacgctc tactatcctc aaacttattg atggagacga
      601 agaaaaggat tctgttattt atgtcaaaaa tgctacagaa ggaataaact tcttagcaaa
      661 attttttaaa gatgaaaatc cagataaatt tgttattacc acagcaatgg agcatatggc
      721 taattacctg ccttttaaag ttaattttcc tacaaaagtt gttggaatta cagaaaaagg
      781 agaacttgat ttatgtcaat tagagaatat acttaaaaat tatgctggta atgtatctct
      841 cgttacagtt acaggagcta gtaatgtcac aggtataacc actcctatat atgaaatagc
      901 aagaatagct cataagtatg gtgcaaagat attggtggat attgtgcaag taattcagca
      961 taaacctttt tcaatgatgc cacatgaatg tgatgagcat atagattttg ttgtttttag
     1021 tgctcataaa tgttattctc cactaaatgg aggagctctg gtaggaccta aaaaattctt
     1081 tgaaaaattt aatcctgtac tatatggctc aggttctacc aaatttgtaa atgataacaa
     1141 aatactatat gcaaacgtac cccaacgttt tgaagcagga tacccagagt attttggaac
     1201 tcttgcaatg ggaaaagcac tgaacacttt aaataaaatt ggtctaaaca gaatttcaaa
     1261 atacgaaaga gaattatttt tatatgctaa agaagaaatg agtaaaatac ctaatataat
     1321 aatatatgga gataaatcaa ataatgtaat tatcccaaat atatctttta atataagtaa
     1381 tatgtattac aaagatgtag caaaatattt agttgataac tttggaatag aaactggagc
     1441 tggagtagta ggtgcagata tttatgtaca aaaattatta ggtataaatc caaaagaggc
     1501 atacgtcaga tttatgaggg aaaatcctgt aggtcttgtt aggataagct taggaatgta
     1561 caatactttt gatgaggtgg atagctttat acatgctatt aaaactttgg cttttaaata
     1621 agatttttct attctttttt atcaataaat agagagtagt tttgttttca caaaaaaaca
     1681 aattatatca acagtcaatt tcattagcaa ttagacactc atttgtaact atacaaacta
     1741 ttttacacta tacaaacaaa aaactcatta caatgagttt ttttgtttat atatgagatt
     1801 tattttaatt acttcaattt actttaatta catatatgat tcaattttgt tttttaaatt
     1861 ctccttaggc ataaaaccta ttaatctatc tacaggtttt ccatccttaa atattagcat
     1921 agttggtact gtagatactt caaatttctg agctaattct atactttggt ctatgtccac
     1981 cttcaagaag tttgcttttt caaccatctc atttcctaaa gcttcatata ttggtgatag
     2041 catcttacaa ggtccacacc aagtcgcaaa gaaatctaca actactaaac catctttatt
     2101 ttctacttca tttataaatt cattattatt tattattttt accataatat acatctcctt
     2161 tattatcttc ttgatattaa tatactacgc cctatataac agttcaatta ggcacttttt
     2221 tgtaaccgcc tatttttaat agatttaata ttatgataaa tagatttata agattaaata
     2281 tttatgagac taaatatttc taatatattt tatttttttc ttaatatagt tgacattttt
     2341 tcccttaaat tataaaatca aattaataac tacataggta gttactaatc ttttatcaaa
     2401 ggagtttcta tatggataat ataattaatg aattacaaaa tataggattt actaaatacg
     2461 aatctcaaat atacatatca ttattaaagg aaagcccttt aacaggatat gaaataagta
     2521 aattatcagg tgtaccacaa tcaaaagtct atgaaaatat aacaaagcta ttaaacaata
     2581 atataattat caatgttgga actgacccta ttaagtacat accaataaac cctaatgaat
     2641 tattaaaaaa taagaaaaaa gaatttgaca attctatgga taatctaaag aatagcttac
     2701 agatattaaa taaaagtaaa agtgtagaat acgtattaaa cataaaagga gataaaaata
     2761 tattaaaaaa agcaatagaa atgataaaaa atgcaaaaca agaaattata atttcttgcc
     2821 cttatgaaga aattcttgaa ttaaaaaaag aactcctaat tgcttacaaa aaaaatatta
     2881 atattgaaat actactttta gaaaacaaaa gtctgaaagg tcttgataat attcatattc
     2941 atggaggtaa taaaactaca cttgaaaata aaatgcttta caaaggaatt attttgattg
     3001 tagataatga tgaaatattg acaggaaact tatcagaagg cagtgaggcc atttcaattt
     3061 ggacaaaaaa ctctaacatt ttatatattg gagttcaata cataaaacat gaaatttata
     3121 tttcagaaca aataaaagga ggttataaaa atgaacaaaa ttaagattaa acaagttgat
     3181 gcttttacaa ctataccttt tggtggaaat cctgctggtg tcgttactga tgcctctaac
     3241 atttctgatg aaattaagca gaatgtggct aaagaaatga atttatcaga aactgcattt
     3301 gtttcagagt caaatattgc agattttaaa gtacaatttt tcacaccagg tttagaagtt
     3361 gatttatgtg gtcatgcaac aataggtact ttccatgcac ttttggaaga agggaagctt
     3421 aatactaata agagtatatt ttatcaagaa acaaaagctg gtgtattatc tgtagagctt
     3481 aagaaaataa ataatgaaaa tatatttatg atggaacaga atacacctga atttgaaaat
     3541 ttagataatt gcagaaaaga acttgcacaa atgataggtg tgcatgaaga tagacttctt
     3601 gaatatccta ttataaaggt aaatactgga ctttggtggc ttgtatttgg tctaaaatct
     3661 ttagatgatt taaaaaatat ctctccagat ttaaataaaa tcaaaaaatt tagtggtgat
     3721 aataatataa ttggtataac tccattttgt atagaaacta tagataaaaa gtgtgactac
     3781 catttaagat ctatagcacc atatgttgga gttacagaag accctgtatg tggaactggg
     3841 aacgcttgcg tatcatctta tatagtacat cacaatattt tagataaaac tagttttatt
     3901 ggtgagcaag gtaactttat aaacagacct ggaaaggtat atgtaaatat aactagagaa
     3961 aacaatgaca taacttctgt aaaaattgga ggaagtgctt atactatttt aagtggtgaa
     4021 attatgtatt aatcccatac ctaataaaaa caaataaaaa tactagctaa ctaaagctag
     4081 tatttttatt tgttattttt atttaataa
//
LOCUS       DAMKMI010000220         4071 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMKMI010000220 DAMKMI010000000
VERSION     DAMKMI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.226"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(145..1062)
                     /locus_tag="PC429_003599"
     CDS             complement(145..1062)
                     /locus_tag="PC429_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF3849052.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
     gene            1324..1701
                     /locus_tag="PC429_003600"
     CDS             1324..1701
                     /locus_tag="PC429_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3849053.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            complement(1857..2306)
                     /locus_tag="PC429_003601"
     CDS             complement(1857..2306)
                     /locus_tag="PC429_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3849054.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(2473..2877)
                     /locus_tag="PC429_003602"
     CDS             complement(2473..2877)
                     /locus_tag="PC429_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF3849055.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            complement(2990..3856)
                     /locus_tag="PC429_003603"
     CDS             complement(2990..3856)
                     /locus_tag="PC429_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF3849056.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRIKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
BASE COUNT         1334 a          681 c          443 g         1613 t
ORIGIN      
        1 agatatgaaa tcttaaaact tttaaatatt gaaatatttc tttatatata ttattgtata
       61 tttcttataa attattgtca atttattttt tcttaaaaat aaaataattg cataatttat
      121 ttcaaattat gcaatttata aaatttattc ccaaaactct tttactgctt cgattatctc
      181 actataaggc aataaagtcg cttcgattat tgatttattt gtcttttcta aataatactt
      241 aatcatataa tatccacaag catatcctgc acaataaggc aaccctactg gaaaatatcc
      301 ttgcatttta gctatatcat caccataaag ataagatgtt atattttgaa atccagtttc
      361 ttttaaagca ctttttatta ttggctttat ataagtattt aatgtttcga tatctgttct
      421 actgacccaa ggtcctaaca tttcctctcc aaacatccat gttgcaaaat tttctgcaag
      481 accttcattt atcatcattt cttctaatgt tatattatta ttccactcta taaactgaaa
      541 tctaatattg tgattacatt catgtgctat taatactggt aatctattga tagtatattc
      601 attaggaacc aatgatagaa atatatatcc aggaatccca ccatcacccc agtatccatc
      661 acttaatatt gtataaggac tatttggatt cgccaataat attgaatatt tatagtcctt
      721 aatatttaaa tcataccctt cttttataaa agcatttata gagttttcta tggtttcttt
      781 acaagtggcc caaattttat cagcagataa cacatttata ttcttttttt gtttttcatc
      841 aatatcctta ggcgataaaa cccctaacat ttcacttgct ataattacat catatccacc
      901 tttctgtcta gcaactattg gaacattcat acacttccac ttaaattcaa aaggcttcat
      961 caattcatat ctgtatatat cttctttttt gtttattggt gcatccatta tcttcttgta
     1021 tattttatct gagcgaattt ctataatatt catatttacc atctccttta agacaaagta
     1081 tatacaatta cgtagcgtca atgtcaatat acatttttta taatgtcacc ttaatctttt
     1141 tattatattt tatcctgtaa accttccgta catataacat ttttttaaat taataaattt
     1201 gtataatata ataataaaaa attatatact tcaaggtttc cctatgatac aatttactaa
     1261 atcataattt agattatcag gtgcaactat aaattattct atatcaagga ggatatattg
     1321 gtcatgctaa ctccatattt aatatttaat ggtacttgtg aaaaagcatt taatttttat
     1381 gctgaggctt tcggaggagg aaaaactata tttgcgcgat tagacagcaa tccaaacaat
     1441 cctattatgc acgcaagtgt tactttcaca aaatacgaag gttgtataat gggtgcggat
     1501 acagacaagc ctgttgtaat ttctggcatg gcgatttgtg ttgttctacc atctcgagaa
     1561 gcgatagaag aaatatctgt aaaacttgcc gaaggtggta cacttgtaca agaattttta
     1621 ccacacccac caccacatca aaatgatggc gctgctgaag tacttgatag gtatgggtat
     1681 acttggtatt taagtacata gttaaaataa aaaaacgaca tctaataatc ataatataga
     1741 acagataaat ttcaatttaa tagtgtagta aaaaagtcgt gtcctaagac tgtcacgact
     1801 tttatttgta taaaaattta agtaaatatt tttgcgaata aatgagaagt ataactttaa
     1861 attaatttca tatgtagtat tggaaatggg ttaccttgtc catctatttc agacctatca
     1921 aacactttaa atcctaagtg aatataaaat cctacagctt ttttattctg ctcatttaca
     1981 tcaacatatt ttacattgta tttatctata gcatatttta ttaatttttt accaatacca
     2041 tttcctctat atttatcttc tataaagagc atctcaattt tatctttatc tattcctata
     2101 aagcccttta ttatatcatt atcttttata caaactatat tttctacttc tttaagtcca
     2161 atttcaacta atggtatcaa agattcaata tctttttctg ttaaaaaatc atgcgttgct
     2221 cttactgatg attcccagac ttgcaacatt tcgtcatatt ctgttgaatt tatttgtttc
     2281 atttcaagaa ttattccaga tttcataaaa atttcctccc tatatcactt ttaattgcat
     2341 aacacttttt tatacaaaac ttagaaagga ctacgaaggt taaatatcat agtcctcatt
     2401 aagtttaaat attatctatt atatattatt atactataat ctagcaaatt cactacaata
     2461 attcacatca gcttatacta aaggtgcttc tatttgagta gtaaaaagga atttatgaca
     2521 atgaccatca ttaacagtag tttctccaca tattaagtga atgtgtttat cagttccagg
     2581 tattctaatt gctggccctg ttgtaacaca tatcttatgg aaatggtcaa ggaaatctac
     2641 attatcattt attttatgaa catgatttgt acctccattt attggtatag cttcaccagt
     2701 aacccctgct gcacggtggt tatgtctatc ctcacattct tctgctaact taacactttc
     2761 tgaatattca tgaacatgtg tcatttcctc ataatcatta tagcacttgt tgcacttatt
     2821 gtatttataa tcatcataca cataatctct ttcacattta taattttgat aactcatttt
     2881 tatatctctc cctatattat attaagaaat tcttgtaaag aatctctaat gtataatatg
     2941 aattaaagtc aaaaaagttc atgttttgta aaattatgta ttaatttaat tatttttccc
     3001 agtatttagg gcgtattagt gaagctggta acttcgtact agaatctcct tttgcagtat
     3061 ttacttgtaa ttgtgttaaa aatatactat tttcaagatt agcacctcta atatcacaat
     3121 ctcttaagtc tgctcctatt aaatttgccc cactcaaatc tgtatttctt aaatttgctg
     3181 ctattagaaa tctacctctt aaatctgctc ctattagatt tatttttcta aggtctgctc
     3241 ctattaaatc tatattttta cttaattttt tcatgttttt aagagtagaa gtcttgataa
     3301 atgatttaac ctttcccatc acagactcac tcgcttgtga taataataaa ttaaccttta
     3361 ttctatgtga tatgatatcc aaattaatta tctgttctgg gctcatatta gttatctttt
     3421 cagtttcttc aattattaaa tcaatttttg atttgattgt atctgatata tttaatacag
     3481 atgcttcttt aagataccat aacatttcat gtaattgtct cataattaaa aacacttcag
     3541 acatttgatt tgttagttct gatgattgca tccagtctat ccccttatat gttacttgtg
     3601 ctactttttg acctgaacca aaacaatcat atgcagtaca ccctttaaaa cctcgcttca
     3661 taagactatt atgtacagaa catttaaaat ctggttgtaa attaatacag ggttttcctg
     3721 attccttatc gataggaaat ccatctgaag cagaaaaata aagagctaca cagcaaagtc
     3781 caaagcactt cttacagtca atctttagct cctcaaataa cttattatca tatatatttt
     3841 tatgtatatc tatcatattt tttctcctct taaactttat ttttaatatt atattatcta
     3901 taactaaatg ataatcaagt cccttattta acagttctta ttctttatta taatttaagg
     3961 tattatttat tccaatagca tacataagtt tctttatact tgtaacaata cctttatact
     4021 aagacttatt ttaaatgtag cactgtaata ataaattgtt tatatactca t
//
LOCUS       DAMKMI010000221         3989 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMKMI010000221 DAMKMI010000000
VERSION     DAMKMI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.227"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(172..372)
                     /locus_tag="PC429_003604"
     CDS             complement(172..372)
                     /locus_tag="PC429_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF3849057.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(597..773)
                     /locus_tag="PC429_003605"
     CDS             complement(597..773)
                     /locus_tag="PC429_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849058.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(827..916)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1096..1296)
                     /locus_tag="PC429_003606"
     CDS             complement(1096..1296)
                     /locus_tag="PC429_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3849059.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1298..1648)
                     /locus_tag="PC429_003607"
     CDS             complement(1298..1648)
                     /locus_tag="PC429_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF3849060.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1810..1999
                     /locus_tag="PC429_003608"
                     /pseudo
     CDS             1810..1999
                     /locus_tag="PC429_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2476..2646)
                     /locus_tag="PC429_003609"
     CDS             complement(2476..2646)
                     /locus_tag="PC429_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849061.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3035..3529)
                     /locus_tag="PC429_003610"
     CDS             complement(3035..3529)
                     /locus_tag="PC429_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF3849062.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
BASE COUNT         1548 a          522 c          429 g         1490 t
ORIGIN      
        1 ggtttaagta catactttat aaatatattt ttgtcagatt tattttataa aaagtattta
       61 gactaagtat tatttttaag tatttttttg ttgaagttat tttttccttt tttacaaaat
      121 gcgtaaataa aaaaaagact cttaaaagag tctttccgaa aaatctatat attataatat
      181 agttatattt tctgcttgag gacctctagc gcctttaact atatcaaagc ttactttttg
      241 accttcttct aatgatttaa atcctgaagt ttgtatagct gagaaatgag caaatacgtc
      301 atctccactt tccattgata taaatccaaa tcctttttca ttattaaacc attttactat
      361 tccattattc attaaaaata ctcctattaa cctatatcaa gatatacttg atttacttgt
      421 gtattaccac aaatactatt gtatcataga ttaacataaa tgtctattta attataattt
      481 ttagttaaca tttttattta ttcttaatat gaaaataaat ttaaacattt tatattacca
      541 atatattaat gtaaatgtag ataaaactaa aaagggtttt ctagaaaaaa atctgtctaa
      601 ttatacttac ttaccaatac catcacttga tgcaagcaag attccacaac tttttctccg
      661 tactctaata taaaatcatc taactgtgta tctataaaat cacaataatc atatgaacta
      721 ccaatataag cagttaacat atgtgtacat ttatccattt tatctctaaa cataatgtat
      781 ttatccccct aaaaaacttt catgaaaaac aaaaagaaaa tatctttttg gcaactggct
      841 gacataactt attttataag cgttagtccc tatagctttg cgtcactaaa tttctctagt
      901 tttgccgatt tagttttatt ctacagatta ataatacaat agaattagtt attattcaac
      961 atatctagct gaaaaattaa taaatttata tatgaacgtt aaattatctg aaaaaataaa
     1021 aagcccctct gtcaggagtg acttaaaaat tgaatttaag tttgtattta aaacacattt
     1081 ttaataatgc aaaaactaat caaaaataaa caattcatca ataggtgtat ctactatttt
     1141 tgatatatct attgctaatt tcaaagaagg attatacttt cctgcttcta aacgcataat
     1201 ggtttctctt ctaactccta ctttttctgc taattgttct tgagttaatt cttttaattg
     1261 tctatatttt tttaggttac aagtgaattt aggcatatta tttttcctct aaaatataaa
     1321 gttttatagc ataaacattt aaagtgattg catatgtaat tgatagtaaa acacacatta
     1381 attctatatt cctagacact atagtaatac accataaaat gaataattct ataatagcaa
     1441 gctgtcctgt aaatgccata gctgcttttt catcttgtac atacctttca tctgctttat
     1501 caccatttat ttttgatata ataaagtttg agaaaaaagc aaaaaatcca atatatgtaa
     1561 ggtcagcaac attgccagat gaaaaatatc taagacttaa aaatccaagg aaaccacaaa
     1621 aacccataaa aaattttttc tgaatcattt tttatcctcc ttagtaattt taaaagagat
     1681 atatttatat ctttatgata caaatatatc tcttttatat tcttttgtca atagaaaatc
     1741 agtattatac aatatttgta ctgcaccata attgttagag caaaaatcta ctgcttgaga
     1801 tgtttatgta tgactaaata tgattttaat ttcaaattaa aagtagctaa attttatttg
     1861 aatagagaag gaagagatat tttaatagta aaacaatatg gagtttataa ccattctcaa
     1921 gttgaaagat gggttaatat attaggagag tgtgcactaa aaataaaaaa aattttctat
     1981 actatatggt tcaagttaga tgttataaac tatatgatga taactaaata tagtgtacaa
     2041 taaatcgcta agtattttgg aataaacaat agcacagtta tagcaacatg aaaataaaaa
     2101 tttttacata gtggtgttga tgccttttct aaaatgaaag gaagaccata atgaataaaa
     2161 acaaaaacac taaagaaaaa ataaattaac aagagaacaa gaattagaat atgaaaataa
     2221 atttttaaaa gatgagcttg agtttttaaa aaagatgtgt gcctcaggga agccacatta
     2281 aaaaacagtg gtatcattat taatcaaaag aaagttagat gtattatcaa aaaattagaa
     2341 cttaaatgca tagcattttt tcataaatca cataaatata actcctataa atgtataatt
     2401 ggaaaagtaa aaaataataa aacaaataga agatttaaaa tatcaatagc atatcagaaa
     2461 ataacaaatg atatgctaga atttataaaa tatatctaat gataaaaatg gaaaatcaat
     2521 tataaaaaag gcatatcccg attcattttt tgttatatag tcaaataaga atactataga
     2581 tgaaagatac gattttgcat atgtattaag taagtattct gatgtttttg gttgatacac
     2641 atgcaataaa gaagttgtgt ttgcagataa attagcaaaa ctatagtata agttgcaaca
     2701 ataaagaaaa tgaaaattat catactaaag cataaggcat gttacaattt tattagatag
     2761 gacattaaca tacattaaag tactttttat tataaagagt acttattttt ttgaaattca
     2821 ttaatgtata aactatcaag aatattactc aacataattt aaaaacaact tgaataggta
     2881 tttttaaatt atgtgatata ataaaaaata gaagtgtatg ccaatacact tctgtaatta
     2941 taactaagca ttctattctt gaggagtgct tttttcattt ctccaaacaa gattgtttat
     3001 ttcatcagca attattaaat aaaatttatt gtatttaagt attcgattta tcatttaaaa
     3061 attttattac aatatctatt ttgttttgct cgttcattat gtctattgaa cttccataat
     3121 cactttttaa tttatctaga ccacttttgc ttatttctcc ttctggaagt atacttaatt
     3181 ctagatattt ttcatgacca ggagatgaca ttaatagata tgaaccatta taaggcatac
     3241 ctaaattttc tattttatct gctaatgggt catctttaaa gccaccattt attattattt
     3301 ttccatcttt ctttacccat gatatcattc ctccatcagt atattttgat tcatctgcat
     3361 agtagctaag agacgcactt tttattgcac ttatatcagc ttcaagttta gctatctttg
     3421 ctttttctat atttttaaat aatgctggaa gtgctactat tgccaatatt cctattatag
     3481 caattactac taataattcc actagagtaa atcctttttt atttttcatt tatttcccct
     3541 ccatataaaa ttaataatat ataaattttt attttgttga tttctacttt ctatatttta
     3601 ccatatatag acaaaaaaat cactttctat gacaaatata ctattatttt acaatttatg
     3661 gaaatgattt aaaatgacat ttcttgttag aaaagatata agaaatggat taaaataaag
     3721 aaaactacag tcatatcaat gtcaataata ttatttaaaa gcagatgaaa ataaaaagag
     3781 gactcagaaa ataatgtcta tttaattata cttacttacc aataccataa cttgatataa
     3841 acaagattat actacatttt ttttatgctc tagtataaaa tcatctaact gtgtatctat
     3901 aaaatcaaaa taattatatg aactatcagt ataataagtc aaaatatgta tacatgaata
     3961 catattttaa aagatatata ttattacat
//
LOCUS       DAMKMI010000222         3965 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMKMI010000222 DAMKMI010000000
VERSION     DAMKMI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.228"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            78..1031
                     /locus_tag="PC429_003611"
     CDS             78..1031
                     /locus_tag="PC429_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF3849063.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1021..1977
                     /locus_tag="PC429_003612"
     CDS             1021..1977
                     /locus_tag="PC429_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF3849064.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            1974..2729
                     /locus_tag="PC429_003613"
     CDS             1974..2729
                     /locus_tag="PC429_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3849065.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2821..3786
                     /locus_tag="PC429_003614"
     CDS             2821..3786
                     /locus_tag="PC429_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3849066.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1607 a          376 c          663 g         1319 t
ORIGIN      
        1 ttattattga taaactataa aagtgaaaat gataataatt ttcattttaa ttaataaaag
       61 atttttaagg agggagaatg aagaaaaggt atttagtcat aggaataata attttgtctt
      121 atttttcttt atttatagga gctgaagata taaatataat gcatatattt gccaaaaatc
      181 agcataaact tatgatattt atcatgagta gaataccaag attaataagt atattgattg
      241 ctggtgtagg aatgagcata gcaggtctta taatgcaaca gataagtaag aataaatttg
      301 tatcacctac aacaggtgct acaatagatg cagcacaatt tgggatagta atatgtatgc
      361 ttttagtacc aactgcctct atttttacaa agacaataat agcatttgta ttttctcttg
      421 tagggacttt tacgtttatg aaaataatag ggaagttaca atttaaaaat ataatctttg
      481 taccattagt aggtataatg tttggaaata taataggttc tatgacggat tttatcgcgt
      541 ataaatacga tttgagtcaa aatgttagtt cttggatgca aggtgacttt tctatgatac
      601 ttaaaggaaa ttatgagata ttatatatca caatacccct tataatatta gcatatattt
      661 atgcaaataa atttactgtt gttggtatgg gtatggattt tgcgacaaac ttaggtctta
      721 gttacaaaag aattgtcaat ataggtctga taatagtagc acttgttaca gtatgtgtag
      781 tcgttacagc tggtaatatc ccttttatag gtctaatagt tcccaatatc gtatccttgt
      841 atatgggtga taatataaga gcaagcatat ggtatactgg tttacttgga gcgatatttg
      901 tacttatatg tgatattttt ggaagaatta ttatttatcc atatgaaata tctatagggc
      961 ttacagttgg agttatagga agtatactat tcctttattt aatattaagg aggaatgtaa
     1021 atgaagctta gtctcaatca aaaaatttac tttgtaagta tattactatt catatcaata
     1081 gcacttttta taatttatgg aatagacatg aatcatttag aatatgcatt atcacaaaga
     1141 ataccaaaaa tatttgctat ggtattaggt ggaggatgta tagcatttac tactgtagtg
     1201 tttcaaacaa ttacaaacaa tcaaatatta acaccaagtg tactaggttt agattcattg
     1261 tatgtaatga ttcaaactat aatagtattc atatttggtt caagcagtag tctgattata
     1321 aatgaaaatt ataattttat tattaatgta gctatgatga ttggagcttc attgttatta
     1381 tataagacgt tatttgaaaa aaataaaaat aatattttct ttttaatcct agtaggaatg
     1441 atatttggaa ccttatttaa aagtgcaaca acatttattc aggtaatgat agaccctaat
     1501 gaatttttag ctttacaaac tagtataatg gcaagtttaa acaatataaa cactaatgta
     1561 ttgttaatag catttataat aattattgcc ataatacctt ttatatatga tgaaataaag
     1621 tatttagatg tattgtcgtt aggtaaagaa caagcaatta atttaggtgt agattttgat
     1681 aaagcagtta aaaaaatgat aatattaata gcaattttag tctcaatatc aacagcactt
     1741 attggaccta tgacattttt aggactttta cttgctaata taacaagaga aatttttaaa
     1801 acatataagc atacatactt gatttcgggt tctatgttga tagggatgat tacattgata
     1861 ataggtcaat tttttataca acatgtattt aagtttgata caactttaag tgtagtaata
     1921 aattttatag gaggaattta ctttattcaa ttactattaa agggggcaaa tagatgatag
     1981 agataaaaaa tatatttaaa agatataaga ataaaaatgt tgtagatgat gtctctttta
     2041 gtatagaaaa gggaaagata acatctttta taggaccaaa tggagctgga aaaagtacag
     2101 tactatcaat agttacaaga cttataggag gtgatggtgg agaagtaata atagaaggaa
     2161 aaagcttgac taattatagc aataaagaat tagcaaaaaa aatagctatt cttaaacaat
     2221 ctaacaatat aactttaaag cttacaataa gagaattagt tggatttgga agatttccat
     2281 atagtgaagg taatctaact aaagaggatg aaaattatat tgatgaagca atagaatata
     2341 tgaagttgac tgatatacaa cataagtatt tagatgaatt gagtggaggt caaagacaaa
     2401 gagcttatat agcaatggta atagctcaag atacagagta catattactt gatgaaccac
     2461 tgaacaattt ggatatgaac cattccgtac agatgatgaa ggtacttaga agtctttgtg
     2521 atgagttaga taagactata gtattagtaa tgcatgatat aaattttgca tcatgttatt
     2581 cagataatat agttgcatta aaaaatggaa aagttaaaaa agtaggtcgt acagatgaga
     2641 tagtaaatga aaaagtttta gaagatattt atgagatgaa ttttaacatt aagaatataa
     2701 atggaaatcg aatctgtatt tatttttaaa atattgtata tgaaaataaa cttgctatat
     2761 gattttttat aaagttttta aattaaaata aagattaata ggagagagga aaatattgat
     2821 atgaataaaa aagcagcaat agtagcagca gtagctatta taggattagt gacagtgttt
     2881 gcattaggtg gaagtaaaaa gaatgaatca aaaacatcag aaaattcaaa taatactatg
     2941 aagataactc ataacttggg agaaacagat gtaaaattaa atcctaaaaa agtagtggta
     3001 tttgattatt cggctttaga tacaatggat gcattaggtg tagctgagaa tttagtagga
     3061 cttccaaaag cgagtttacc agcatcctta gaaaaatata aggatgacaa gtatgcagat
     3121 ttaggaggat taaaagaacc agatttagag ggtataaagt cggcaaatcc agatttaatt
     3181 ataataaatg gaagacaaga agatttttat gagcaattgt caaaaatagc accaacaata
     3241 agtacaagta aagatgataa gaagtactta gaatctgtta aaaataatat agacaaaata
     3301 gcaaaaatat ttggagtaga agaaaaagca aatcaagaat ttagtaaaat tgaaaagaaa
     3361 atagaaactt tgaataaaaa agtaacagat aaaaatttaa atgctttaac tataatggta
     3421 aatgaaggta atttaagtgt atttggtgaa gaatcaagat ttagtatatt atacaatagt
     3481 tttggatttg aaaataaaga taaaaatata aaagaatcaa gtcatggaca aaatattact
     3541 tttgaatata tagctaaaca aaatccagaa gttatgttcg ttatagatag aggaatagct
     3601 actggcagtg atgtaaaaga aagctcaact gcaaaatctg ttttgaacaa tgatataata
     3661 aaatctatgg atgcatataa gaatgataat ataatatatt tagattcacc aacatggtat
     3721 gtaaatgatg gtggtctaac atctttaaat aaaatgatag atgatgcttc aaaagcggta
     3781 aattaataat ccgatataat caaaatatat tttttataaa atgattatca aataaactag
     3841 gagtttttaa gaactcctag tttatttttt gcataaaaaa gatatagttt aaacaataat
     3901 attatctata ataagataaa tctattcaaa atctaataaa atatgaagtt atactagaca
     3961 aaaag
//
LOCUS       DAMKMI010000223         3916 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMKMI010000223 DAMKMI010000000
VERSION     DAMKMI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.229"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..594
                     /locus_tag="PC429_003615"
     CDS             <1..594
                     /locus_tag="PC429_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF3849067.1"
                     /translation="IIAVIFIISVTMLILISHINILIIADIKNKDICLKLNIKYMFNL
                     ININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKIKIHELYSNINFG
                     TGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGNIRIHIRPRIKAL
                     FNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            548..976
                     /gene="ytfJ"
                     /locus_tag="PC429_003616"
     CDS             548..976
                     /gene="ytfJ"
                     /locus_tag="PC429_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF3849068.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1224..1664
                     /locus_tag="PC429_003617"
     CDS             1224..1664
                     /locus_tag="PC429_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3849069.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1680..3131
                     /locus_tag="PC429_003618"
     CDS             1680..3131
                     /locus_tag="PC429_003618"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF3849070.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3222..3890)
                     /locus_tag="PC429_003619"
     CDS             complement(3222..3890)
                     /locus_tag="PC429_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF3849071.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1634 a          358 c          670 g         1254 t
ORIGIN      
        1 ataatagctg taatttttat tataagtgta actatgttga ttttaatatc tcatataaac
       61 atcttaataa tagctgatat aaaaaacaaa gatatttgtt taaagcttaa tataaaatac
      121 atgtttaatt taataaatat aaatagacaa ctttatccag ctgaaaatag taaaaataat
      181 gataaaaaag aggggatgaa gaataatata gattcaagta tattattagc tgatgacctt
      241 ttgagcattt atagattatt aaaaaagata aaaatacatg aactttattc aaatattaat
      301 tttggaactg gaaatattgg cttaacatct tctgtatatg tattgataaa tactttgtat
      361 ggaaatttat ttaatatgat tgatgcagaa aaaatgtatt taaatgtaaa tccagatttt
      421 acaaaagatt atgttttagg aaatataagg atacatataa ggccaagaat aaaagcttta
      481 tttaatatta taataatgat taataaaatt atgaataaaa ataaaggaaa taaggaaggt
      541 gatagtaatg agagcaacag gttcgataca gagtcttatg gaaacaactc ttgaaacaat
      601 taaaggttct atagatgcaa atactattat aggagaccct ataaaaacag atactacagt
      661 ggttgtacct atatcaaagg ttacaattgg ctttggtata ggaggaggag aatactcaaa
      721 aggatatgat gataaagata gggaaattga attaaaaaat gaaaaatcag atacgaattt
      781 tgctggaggt agcgcagggg ctatatctgt acaaccagta gcatttgtag tagttgaaag
      841 tggagagact agaataatga gtttagacag taatatcaat ctagtggata atatattatc
      901 tataactcct agagtattgg agaaaattca aaatatatca caaagtaata agaatcaaga
      961 taaaaacaat atgtaagaat aaaatatttt taaatatgta tatagtttta ggtaaagtag
     1021 tggggttagt ataaatccca ctattttgtt gtgtacttta aacatttata tctaataaga
     1081 ttttagttta aaacaattta aatagataat aaagttgata tatagtataa ttataaaaag
     1141 aaaagaagta ttatattata gatatataaa cgcatttgag atataagagg gagttataaa
     1201 tcattagttt tgaggtgaat gttatgagcg ataaagaatt atctattttg ttgttagaaa
     1261 gtatgaataa ttttcataat ttaataaaga ttattaataa cgaaagatat aaaaaagata
     1321 aggttcttac agaaagacag ttctttgcct tagtaaaaat aagaaaacat gataaaatag
     1381 agcttaaaaa tctaagtaga gatttgcatg tatctacttc tagtctgtgt atactactta
     1441 ataaattggt tgagcaagag tatgtatata gggaagaaga tagtagagat agacgaaata
     1501 ctttttatgg aataactaaa aatggagaaa agatattgga taatgaaata ctaaagtttg
     1561 tatctataat aagtgataaa atggattgcc tagatataga taataaagat aaattattta
     1621 cttctttgga agagtctaaa aatataatag aacaattatt ttaaaaagga gaatttatta
     1681 tgaatataaa gtattctaaa gaaatattaa aaagtttaga aaatgctata acagtagtag
     1741 ctctatttga gggagaagat gtttctacga catttgataa aataaatatg ataagcagaa
     1801 attttaattc taaagaaaat agtatagata ctgtaacatt gattgaaaat aacaatttgg
     1861 taaatgtttg tcttgttggt tttggtaaga gagagttatt gaatagagaa aaaattagaa
     1921 tcataggtgg taatttgtgt aaaaaattaa aaaatatatt gaaaaatgaa gcatataaga
     1981 gtagtgatgt taatgtactg aatcttaatt taaatagtga tgaaataggt tcttttgtag
     2041 aagggatatt acttggaaat tataagtttg ataagtataa gacaaaaatt aaggattttg
     2101 aaagaaaaga agttgatact ataactatat ttactgaaaa agaagttgag tgtcaaatag
     2161 aaaaggctaa aatattagct aattcaacta taatagctag aaatctagtt aatgaaccat
     2221 caaacgtaat atatccaaaa acattagcag tagaaacagt taaacttggt gcagagtttg
     2281 ggtttaatgt tgatgtttat gaagaagaaa aaataaaggc attgggaatg gatgcttttt
     2341 tcgctgtttc aagaggctca attaataaac caagattcat agtaatgaga tactttggag
     2401 ataaagaaag tacagatata ttaggtttag taggtaaagg attgacttat gatactggtg
     2461 gatattcttt aaaatcaaat gcttctatgc tagatatgaa gacagatatg gctggtgctg
     2521 cgagtgttat aggtgctatg tgtgctatat ctcaaagtaa attaaagaaa aatgttatag
     2581 cagtagtggc tgcatgtgag aatgctttat caggaggttc atacaaacct ggagatataa
     2641 ttagttctat ggcaaagaaa actattgaag ttttaaatac ggatgcagaa ggtagactta
     2701 cattagctga tgcaatatac tacattataa acaatgaaaa agtaacaaag gtggttgatg
     2761 tagctacatt aactggtgct gctttaacat tgttaggtaa tgttgcaact cctatagtaa
     2821 caaataatga tgatttttat tgtgaattag aaaaagctgc cactttatca ggggaaaggg
     2881 tatggaagat gccaatttat gatgaattta aagatatgat aaaaggagaa gaagcagacc
     2941 ttaaaaacac tggtggtaaa aatgctggat gtataacagc aggagctttt attggtgaat
     3001 ttgttgggaa tactccttgg atacatatgg atatagcggg gacaagtaca tcatcaaaat
     3061 caattggata caaagcaaag ggggctacag gagaacctgt tagaacattg tactatttgg
     3121 cagaaatcta aaaaacaatt ataaaatata atttataaag ccacgtggat atttttgtaa
     3181 tttaagatta tctatgtggc tttatttttt atttaaataa attatttact ctttttcttt
     3241 ttttcaagta taaaagtatc tctattaaat aaaagagcag gtatgtacat aatactagta
     3301 acaaaaataa acgtaaaaat agcagttgtg ctatgactta aaaaaccata atagtaccta
     3361 gagttggtga atgtgtttag taaaaatatg aatgaaaaat aaataaaaca tatagctaag
     3421 ttatctctaa gtgcatatct gttaactcta aattttctaa catcaaccat atatatatct
     3481 gcataaatta tcaagcactt agagaagata aagataaaat atattaagct aggaaattca
     3541 tagcccaagt ttttagaaaa aatgagttct ccaactgaac aaactaaact ccaagaaaaa
     3601 aatatattaa aaagatgata ctgcttaaaa attagtatgg ctatacacag atatatacaa
     3661 atataattga ttccaattgg taaagagttt agagtactat aattacctga agaaataagt
     3721 aataattgct ctagagccat aattattaca agtaatgtac aaattatctt ctcaactgta
     3781 tagctataag gtaaaatatt tttggttagt tttggacaat agtataaaaa taatccaaat
     3841 ataaagagac ttacaaattg ttcactagaa aaaagaaaaa ttttaaacat ataacattct
     3901 ctcctataat aaattt
//
LOCUS       DAMKMI010000224         3812 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMKMI010000224 DAMKMI010000000
VERSION     DAMKMI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.230"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            272..1852
                     /locus_tag="PC429_003620"
     CDS             272..1852
                     /locus_tag="PC429_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF3849072.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            2030..2476
                     /locus_tag="PC429_003621"
     CDS             2030..2476
                     /locus_tag="PC429_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF3849073.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2497..3540
                     /locus_tag="PC429_003622"
     CDS             2497..3540
                     /locus_tag="PC429_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF3849074.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1550 a          345 c          708 g         1209 t
ORIGIN      
        1 gaaaaatata aataagtcat aaaagattat aaaaataaag agatatttag ataattagtg
       61 taaataggtt ttaaatcaac ataataactt aaattatatg gtataataaa ttaaataatt
      121 gttttaattc aaaatttttt gttattttaa attatttcaa aactcttagt tttagtgatt
      181 ttatttatga tatttatggg attaaaaaga tttcaataga tatttttggt aagtatttaa
      241 gaagtcttta actttatagg agtgataatt tatgctaata ggtacatcta aattaagcag
      301 aaatgttgat actgcatgtg tagaagaagt agggattcca atgattgtat tgatggaaaa
      361 tgcggttata tctgcaatga aaaatatgga tatagacatg tataatagtt atacagttgt
      421 atgtggtgtt ggaaataatg gaggagatgg tcttggaata gcaagacatc taaatacata
      481 tggaaaagaa gtaaacgtat ttttagtagg taatcttgaa aaactaagtg aatgctctaa
      541 aataaattac aatgtacttt taaatatggg aattaatatt ataaatatta ataaaaatga
      601 tttaagtgaa aaaaattatg gagaggaaaa tgaagtagat aaaattatta aagttgagaa
      661 acaaaatatt ataaaaaata aaaatttaac aatttttaaa gatagagtat ctaaaagtga
      721 tgttgtagta gatgctatat ttgggactgg attaaaacgt gatataactg gtatttttaa
      781 agaagttatt gacatagtaa acgataatag caaaaatacg tattctatag atattccttc
      841 aggtatagat agcgataatg gaaatgtatt aggaacttgt attagagcaa acaaaacgat
      901 tagctttgag ttttataaaa gaggttttct aaactatgac actagcgcac ttataggtga
      961 tgtagttgta gaaaagatag gtgttccaga ttttattaca agaaaatacc atgatgaaga
     1021 gttcataact gataagactt ttgtaaaaaa taatattaga agaagagata agcatggttt
     1081 taaaagtgat tttggaaaag tatctatagt tgcaggttca aaaggatttt atggtgcaag
     1141 tttcatagca actgaatctg ctgttaagag tgggagtgga cttgtaacat taattagtag
     1201 tgaagatgtg caagaaaagg tatctattag acttacagaa gcaatgactt taaattttga
     1261 agaagaaaga ttaaataaat tacttaattc tagtgatgca attggttttg gtccaggtat
     1321 gggagataat agtcaaacat ttgataaatt acttaagata gtagaaaatt ctaattgccc
     1381 aatagtatta gatgcagatg gattgaacgt tatgaaggac agatgttata agtttttaga
     1441 atggaaaaat agatttgtaa ttacacctca tttgggagaa atggccaggt tgacaggaga
     1501 ttcaattggg tatataagag aacatagagt agatgttgca aaagaatttg ctcaaaaata
     1561 taatctggtg gtgcttttaa aaggttatca aactgtcata acagatggta aaaaaacata
     1621 tataaatcct acaggaaata gctgtatggc aactggtgga atgggtgatt gtttgcttgg
     1681 aatgattact tcatttatag gtcaaggtat ggatatattg gaagctacag ttagtggagc
     1741 atatatacat ggctatatag gagataaact gagcaaagca atgtatacgg taaatgctac
     1801 ggatttaata agtaacattt cattaacaat gaaagagttg ttggatatat aattggtgat
     1861 atttgatact actttgtaac aaatttaaca aagtatgttg atataatact attaattaat
     1921 taattaattt attttattgt tttgtaattt aaatttatat tcaaaaacat tgtataaaca
     1981 tataatttat atagggtttt ataattcata tgaaaagagg gtagatagta tgaagttaat
     2041 atggttgata gtagcagtct tatttggaat agcagaaatg ttaacaccaa gcttaacatt
     2101 gatatggttt agtgtaggag ctgtaatact tatattttta agtagcttta tagagagtat
     2161 tttcttacaa attttaatat ttgcagtaat ttctatagca atgttagtcg tagctactaa
     2221 aaaaatagta aaaaaagata aaggctataa atctaacact aatttacaag caatgatgtc
     2281 taaaaaaggt attgttacag aagagatttc tccaaataac acaggtctag ttgtagttga
     2341 acatgaaaca tggacggcta tttctataga tggtgaaaag atagaaaaag gttctacagt
     2401 agaagtatta aaaattgaag gggtaaaatt agttgttaaa aaagtagacg ccacagttaa
     2461 tgtgacaaac caatagaata aaggaggatt tatagtatgg gtactaaaat tgttttaagt
     2521 attgtattaa tagtagtagt cgtagcaata agtttaactt gtataagagt tataaaacaa
     2581 tctaaagtag gtataataat gagacttggt aagtttcaaa aagtagctga aacaggtgta
     2641 catttcttaa taccattttt agataaaatg gcatatgtaa ttgaccttag agaaattgta
     2701 atagattttc cacctcaacc agttataact aaagataatg taactatgca gattgataca
     2761 gttgtatatt ataaagttac agacccagtt agatatgtgt ttgagatagc taatccaata
     2821 gctgctattg aaaatttaac agctacaaca ctaagaaata taattggtga acttgattta
     2881 gatgaaacat taacatcaag agatataata aatgtaaaaa tgagaactat ccttgatgaa
     2941 gcaacagata aatggggtat aaaagtaaat agagtagagt taaaaaatat aatgcctcct
     3001 caggatattc aagttgcaat ggaaaaacaa atgagagcag aaagagaaag aagagaggca
     3061 atacttcaag cagaaggtaa taagtcagct gcaatacttc aagcagaagg tgaaaaacaa
     3121 tctgcaatat taacagcaga agccaaaaaa gaagctatgg tacgtgtagc agaaggtgaa
     3181 aaagaatctg ctatattagt ggcagaaggt gaagctgaag ctataagaca gacagctatt
     3241 gctaaggcac aaggtgaagc tgaaatgata aaaagaactc aaatggcaac agcagaaggt
     3301 ttaaaattag ttttttcagc aatgaaagag gctgatatag acaataatat tctagcatta
     3361 aaatctatgg aagctcttga aaaaatggct gaaggtaagt caacaaaact ggttttacct
     3421 tcagaagcag ttaatttcct aggaacattc aaaggaataa aggaagttat gagtgatgat
     3481 aacaaagaag tacttgatat aaaagaagtt ttaaatgata atgaatcatt aaaaaaataa
     3541 aagagttttg ttatcatagg aaggatatgg aaattaaagg tcgaattaat taaatatagc
     3601 acaaaaatgc acgttgaaaa gaagaggact tataaatttt taagtcttct tctttgtttt
     3661 tattagaaag atgaaattat aaaaataaat taacagaatc tttatacaat ttatccttta
     3721 ctaatgtaaa aagtgttata taatataata taaaacatat agcaaataag catggtttta
     3781 gttagtctat ttatattatt aatatatatt ta
//
LOCUS       DAMKMI010000225         3750 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMKMI010000225 DAMKMI010000000
VERSION     DAMKMI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.231"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            22..690
                     /locus_tag="PC429_003623"
     CDS             22..690
                     /locus_tag="PC429_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849075.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            849..1355
                     /locus_tag="PC429_003624"
     CDS             849..1355
                     /locus_tag="PC429_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF3849076.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1483..2499
                     /locus_tag="PC429_003625"
     CDS             1483..2499
                     /locus_tag="PC429_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF3849077.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2853..3557
                     /locus_tag="PC429_003626"
     CDS             2853..3557
                     /locus_tag="PC429_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF3849078.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1604 a          323 c          655 g         1168 t
ORIGIN      
        1 ttttttaaag ggtgattttt tatggaaaat ataactagtt attttacaac aatactatgt
       61 gtatttatat gcttatcatc tgtatttata tttactcaac tagctagagt atttattaat
      121 aagaagaaga ttaatcaaaa aattaaatca agaaacggat ttaggtatga tagagatttt
      181 attgaagcca gaagagaaga aatacatata aaggataaca ataataataa aaataagagt
      241 aacaataaga aattaaagga agaaaaagta tttaaatacg ataatggtga tttatataaa
      301 ggtgagtttg ttgatggaaa gaaaaatgga tttggaatat atattttttc aggtaaggaa
      361 aagtatgaag gattatggaa agatgacaag atgcatggaa taggaaaata tacttataga
      421 gatggttcaa tatatacagg tgaatttaaa tacggattaa aaaatggttt gggaaaatta
      481 acatatccca ataatgacat ttataaggga tattttttag ataataagaa aaatggtaag
      541 ggtgtattat ataaaaatga tggaaataaa caagcaggaa tatgggaaga tgatgaacaa
      601 tgtaaaagtt tagattttaa agatttaaat aataataaaa taaataatgt ttatcaaaat
      661 agattaaata acaataaatt aaaagtttaa ttattctaat aaaaaaatct ttatttatga
      721 tggaatatag gatatatcta tgttgtaatt tgtaaaatct tcctatatca tataaaatta
      781 agtattgatt agtctattac ataaatatag aatataaata tatccattta ataataagga
      841 gaagagatat gaaagagtat gtagttgtgg atttagaaac tacaggatta gatccatata
      901 aaggttgtga aataatagaa ataggaataa cagaaataaa aaatgaacaa attgtaaaaa
      961 actattcaag gttgataaaa cctaagggga taatttcttc atttataaca gaattgacac
     1021 atataagtaa tgaaatggta gaaaatgaag aaccactaga attagtttta cctagattta
     1081 gaaaatacat aggtgataga actataattg cacacaatgc gaaatttgat ttaaaatttt
     1141 taaattacta tcttagaatg ttagatttag aaccaataaa caattatata tgtacagtag
     1201 agttattaaa aaaatgtaaa agttataaag gaaaaaacaa gaaacttgaa acagcttgtg
     1261 cttattacaa catagagaat ataaatgctc atagagctga cagtgataca ttagcaactg
     1321 caaaactatt tttgaaaata aaagatgaat attaattata aatatagaga aaattagtga
     1381 aaatatcctt tataaaattg acccttaagc ttacaatttt gttctttgat ttattttgta
     1441 gaacaatgta taatgtacct tatatgaaga agggagacta aaatgggtta ttacaatgat
     1501 aatgataata ataaaataag acgtaaaaaa attagtaaca acaaagatag taataaaaaa
     1561 tctaaacatt cttatacttc ggcaaataaa aaaaataaaa gtcctaatac aagttccagt
     1621 aaaagaatga gtgaaaaagt agattcacag aatcttataa aaagagatag agatagtttt
     1681 acaagagata atgtaagacc tttaaataca gattacaatc aaagagaaag aagaagagtc
     1741 gagtataaac aaagagagaa aaaattaaaa agtaaaaaaa gaaaaaaaca aaaaaggatt
     1801 agaatagtta gggcattatt atctattatg ttagttgcac taattatatt ttcagtggca
     1861 ggtgcaatat ttacagtttc agctataaaa gggtcaccag aagttactaa aaagttaatt
     1921 aggtctaaat atataagtag tgaggttgta agtataaaac aaatgccaaa tgatttaaaa
     1981 catgcaatag tttccataga agatgagaga ttttataagc ataaaggagt agatgttatt
     2041 tctctagtta ggtcagtatt aaacaatgtg tttacagata caacacaagg tgcaagtact
     2101 atagatatgc aagtttcaaa aaacctgctt actagtaatg ataaaactat gaagcggaaa
     2161 ataagggata tgtataatgc taagcagatg aataagatta tgactaagga tgaaattctt
     2221 gaagcatatt taaataatat gtatctaggt aaaaatgcat atggagctgc aaagggagct
     2281 gaagtctact ttggtaaaaa agttagtgaa ttaaatttag ctgagtgtgc tatgttagct
     2341 ggtatgacaa ataatcctgc aaggtatatt gaccatggag aggctaagaa gagacaagtg
     2401 attgtactgt ataagatgca tcagcttggt tatataactg ataaggagta tagaatagca
     2461 aaagctgatg acactccatt taaatcagaa attgattaaa attgtaagaa tatagtattt
     2521 tcaatagttt aaacaaacaa aaatgtaata aaaaaggtga tttgccaccc atttgccacc
     2581 ataattataa tctaatggca aattgtttta atgtacaaac tagataatag aatattgtct
     2641 taaatgctat attatgctaa aatttaattg tattttgtaa attaaaaaac ttataattta
     2701 gttttctagg gttccgcatt ttataatggt caggtccaag agaaaacgcg caattgttgt
     2761 gtacacggaa ggataaaagc ctgggagata tttaagaata tttctatagg cttttttatt
     2821 tttacatatt ttaaatttga ggaggttttc taatgaaaaa atttgttatt gaggatgcat
     2881 tttggaatct atttcctagt gcaaaaattg gagttgttat ttgtaatgat atagataact
     2941 ctataaaaga tgaagactat aagaaaataa ttttgaaggg agaagaagaa gcattaaaat
     3001 acttggagga ctcagaattt agcaacaacc aagtgattaa agtatggaga gaagcattta
     3061 aaaaatttaa aacgaaaaaa ggagcaaggt cttctattga agcattatta aaaagagttc
     3121 ataatggaaa taatatagga actattaacc ctttagtgga tatctacaat tttatctcat
     3181 taaaatatgc attaccttgt ggaggggagg atatagataa atttatagga gatattaggc
     3241 taactagagc aattggtaat gaggagttta tcccactagg gacagatgaa aattcatttc
     3301 catatgaagg agaaataatt tataaagatg atggtggagc aatttgtaga tgttggaatt
     3361 ggcgtgaagc agtgagaaca atgcttactg aagatactaa caatgccttt ttatgcattg
     3421 aattagtaga tgaaagtaga tttaaagaat ttgaaaatgc tttaaatgaa ttagctaaaa
     3481 tagttcaaga aaaattgggt gggaattgta aaatttcaat tttagatatt gataataaag
     3541 aggtatcagt ggattaatat ggaggtgctg ttgtaatgga ctattctagt tataaagata
     3601 cttatattta gggtaagaag gtctatttta gatataacga ttatcaaagg atataatata
     3661 gaaaataaaa atgtagaata taaaaagcct ttaaaataag aaaaaaatgt attttaaagg
     3721 cttttattat actctttttt atttgcataa
//
LOCUS       DAMKMI010000226         3686 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMKMI010000226 DAMKMI010000000
VERSION     DAMKMI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.232"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            261..620
                     /locus_tag="PC429_003627"
     CDS             261..620
                     /locus_tag="PC429_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3849079.1"
                     /translation="MKLRESYTCPLELVHDIIKGKWKTIIIFQLRNGKCSFSELHHSI
                     AGITQKMLLEQLRELKHFGLIDKKTYDGYPLKVEYFLTGQGEKMLSAIKIMQEIGIDY
                     MVEHGMTEILDEKGICY"
     gene            710..1261
                     /locus_tag="PC429_003628"
     CDS             710..1261
                     /locus_tag="PC429_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF3849080.1"
                     /translation="MKKALLIIDVQNDYFPNGKCQLYKPEIALNTIKDLLKIFRKQNL
                     PIIYIRHESIHGTFFNPNTNGVQIHNDIKPLDTETVIVKHYPNSFYETNLQNKLVEIE
                     VTKLVVCGMMTHMCIDTTIRAAKDYGYEITLISNGCATKELKWNGVNIPADTVQSIFM
                     ASLNQKFANVITSTEFLSNEITR"
     gene            1245..1610
                     /locus_tag="PC429_003629"
     CDS             1245..1610
                     /locus_tag="PC429_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3849081.1"
                     /translation="MKLRDEYTCPLELIHDMIKGKWKPIILWRLRLGATSLSKLENDI
                     DGITQKMLLEHLKELIDYGFVDKKTFSGYPLHVEYFLTEDMGNQILEALRIMQHIGIE
                     YLRKNGMENELTEKHVIPD"
     gene            1698..2198
                     /locus_tag="PC429_003630"
     CDS             1698..2198
                     /locus_tag="PC429_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF3849082.1"
                     /translation="MYISSIGISNGIIEKKYGSHGVQFNENDVPTYSIPFKIENAPDK
                     TVSFAFILEDKDAYPVTGFIWVHWLGANLTRSELKENESQTAKDFIQGANSWTSILGN
                     QQTREQTSCYGGMAPPDKAHIYELHVFALDKLLDLKNGFYLNELYQEMEGHILEEAVL
                     KGLYEK"
     gene            2188..2703
                     /locus_tag="PC429_003631"
     CDS             2188..2703
                     /locus_tag="PC429_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF3849083.1"
                     /translation="MKSKPVKVGLRTIKTVLSAFLCFLIDSLRASSVPFYAVIASILC
                     IQPDMENSLSVAKNRELSTIIGGILGMLFLLMERIFLPIQPEILRELTLSILLIPIIN
                     LSVLLKQKKGTFLTCVVFLCVTVTHEKDISPIWFAWNRIVDTSLGIGISLAVNSFPFH
                     KFKVGGTMGKY"
     gene            2697..2978
                     /locus_tag="PC429_003632"
     CDS             2697..2978
                     /locus_tag="PC429_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3849084.1"
                     /translation="MLIVEFTEKEDAVFQQAVEWLSSKLAYTPASFNQQKKGFDIDPL
                     TRTFTNEEGTSIYLTAKEFDLLYFLSSHKGQVFTKEQIYEKVWGYDNFY"
     gene            3346..3570
                     /locus_tag="PC429_003633"
     CDS             3346..3570
                     /locus_tag="PC429_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849085.1"
                     /translation="MIGIKKRDIKKALKAGAKAGGSSYADVLADIEATIDEVMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTRV"
     gene            3592..>3686
                     /locus_tag="PC429_003634"
     CDS             3592..>3686
                     /locus_tag="PC429_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF3849086.1"
                     /translation="MKNEMWVLCPICNNKTRTKVREDTELINFPL"
BASE COUNT         1329 a          501 c          721 g         1135 t
ORIGIN      
        1 ttccccatag aataccttat cgcctaaatc attacgagaa agcccagctt tttcacgggc
       61 ttctctgatt gccagcccta gcggtctgaa atcaaaagta tgtgtttctt ttgtttttct
      121 cattttaaat caccatacat gagtttatac ttcatggttc gtatttagaa tgtgttaaaa
      181 gtgcatatat tagggtaata aaatatgttt tgaaatattg agcttaaaca tgtaagatat
      241 ttcacatagg agatgataaa atgaaactta gagaaagcta tacatgtcca ttagaacttg
      301 ttcatgatat tataaaggga aaatggaaaa cgataattat ttttcaactt agaaatggaa
      361 aatgctcttt ttccgaactc catcacagta tagcaggaat tactcaaaaa atgctattgg
      421 aacaactgcg tgaattaaaa cactttggct taattgataa aaaaacatat gatggatatc
      481 ctctaaaagt agaatacttt ttaactggac agggagaaaa gatgctatca gcaattaaga
      541 taatgcaaga aattggtata gattacatgg tagagcatgg tatgacagaa atccttgatg
      601 aaaaaggaat ttgttattaa actctatcat atacacgaaa aagtaagtaa tttacttaga
      661 aactattatc ttatattatt taattagaat taagagagga tatgagagta tgaaaaaagc
      721 attacttatt attgatgtgc agaatgatta ttttcctaat ggaaagtgcc agttgtataa
      781 accagagatt gcgttgaata caataaaaga tttattgaaa atttttagaa agcaaaactt
      841 gccaattatt tacattcgcc atgagtctat ccatggaaca ttttttaatc caaatacaaa
      901 tggtgtacaa atccataatg atattaaacc attagataca gaaacagtta ttgttaaaca
      961 ttatccaaat agtttttatg aaacgaattt gcaaaacaaa ctggtagaga ttgaggtaac
     1021 gaaattggtt gtttgtggaa tgatgacaca tatgtgtatt gacacaacta taagagctgc
     1081 caaagattat ggctatgaga ttacactaat atcaaatggt tgtgcgacca aggaattaaa
     1141 atggaatgga gtcaatatcc cagcagatac agttcaaagc atatttatgg cttctttaaa
     1201 tcagaaattt gctaatgtta ttacaagcac ggagtttttg tcaaatgaaa ttacgagatg
     1261 aatatacatg tcctttggaa ttaattcatg atatgataaa gggaaaatgg aagccaatta
     1321 ttttatggcg cttacgatta ggtgctactt cattatctaa attagagaat gatattgatg
     1381 gaattacaca aaaaatgctt ttggaacatt taaaggaact tatagattat ggatttgttg
     1441 ataaaaaaac attttctggt tatccacttc atgtcgagta ttttttgacg gaagatatgg
     1501 gaaatcaaat actagaagca ttacgaatta tgcaacatat agggattgaa tatttaagga
     1561 aaaatggaat ggaaaatgaa ttaactgaga aacacgtgat tccagactaa tacctacaaa
     1621 aaagtgggta atttacgttt tgcttcctct ttgttaatat atatctgtaa ttaaacgata
     1681 tacaaggagg aaaatttatg tatatttcaa gtataggaat ttctaatgga attattgaaa
     1741 aaaaatatgg tagtcatgga gtgcaattca atgaaaatga tgtgccaacg tattcaattc
     1801 cgttcaaaat tgaaaatgca ccagataaaa cggtttcctt tgcctttata ctggaagata
     1861 aagacgcata tccagtgacg ggttttatat gggtgcattg gttaggagct aatctaacaa
     1921 gaagtgaatt aaaggaaaac gaaagccaga cagcaaagga ctttatacaa ggtgcgaata
     1981 gctggacaag catattgggg aatcagcaaa ccagagaaca gacaagctgt tatggtggaa
     2041 tggcaccacc agataaagca catatttatg agcttcatgt atttgccctt gataaactgc
     2101 ttgatttaaa aaacggattt tatcttaatg aactctatca agaaatggag gggcacatcc
     2161 ttgaagaagc tgtactaaaa gggttgtatg aaaagtaagc ctgttaaagt tggattgcgg
     2221 acaattaaga cggtactttc tgcgttcctt tgttttttga tagatagctt aagagcttca
     2281 agtgttcctt tttatgcagt gattgcgtct atactgtgca tacagccaga tatggaaaac
     2341 agtttgagcg ttgctaaaaa cagggaactt tcaacaatta tcggcggtat actgggaatg
     2401 ctctttttgc ttatggaaag aatttttttg cccattcagc cagaaatact tcgggaattg
     2461 actttatcta ttttattgat tccaatcatc aatctgtctg tactgttgaa acagaaaaaa
     2521 ggaacatttt taacgtgcgt tgtattctta tgcgtaacag taacacatga gaaagatata
     2581 agtcctattt ggttcgcatg gaataggatt gttgacactt cattagggat tggtatttca
     2641 ctggcagtaa actctttccc ctttcataaa tttaaggtag gtggtactat gggaaaatac
     2701 tgattgttga atttactgaa aaagaggacg ctgttttcca acaagcggtg gaatggctgt
     2761 cctctaaact tgcttacacc cctgcttctt ttaatcagca gaaaaaagga tttgatatag
     2821 acccattaac ccgtaccttt accaacgaag agggaacgtc tatatatctg actgctaaag
     2881 aatttgacct actatatttc ctttccagcc acaaaggaca ggtgttcaca aaagaacaga
     2941 tttatgaaaa ggtatgggga tatgacaatt tttattagtc tggcaataat gaagaaaaaa
     3001 gaattttaga aaagtgaggt gtgcttatgg atttatctat tttggtagta gtattgatat
     3061 tgggtgtggg tattccttta agaaataagg caatcaaaaa atacagataa aatatgaaaa
     3121 agaaaggaaa aatgaaaaaa tatgaatatt tagaaattga ttatactgcg aaaggtgttg
     3181 tattcctttg caccgataaa cataaggaag ttataaatag ctatgcagaa aatgggtatc
     3241 gctatgcggg ttcgtgccta cagaaattga cgcaaaaggc tgtatgcgaa agatagattt
     3301 aatttttgaa aaagaagatt aacgtatttt agggagttgg ttcttgtgat aggaattaaa
     3361 aaacgagata ttaaaaaagc attgaaagct ggtgcaaagg ctggtggttc ttcgtatgct
     3421 gatgttttgg ctgatattga agcaacgatt gatgaagtta tgaacagcac cgacccagaa
     3481 gtgcaggcaa atttcaaaaa gtattttgga aataaacgcc ctacaccaga ggaatatatc
     3541 tataaaatca cgaagaaaac acgagtttaa agaattagta agggtggttc tgtgaaaaat
     3601 gaaatgtggg tattatgtcc tatttgcaat aataagacac gaactaaagt gcgagaagat
     3661 acggaattga taaattttcc tttgtt
//
LOCUS       DAMKMI010000227         3662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMKMI010000227 DAMKMI010000000
VERSION     DAMKMI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.233"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            128..475
                     /locus_tag="PC429_003635"
     CDS             128..475
                     /locus_tag="PC429_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF3849087.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(625..1125)
                     /locus_tag="PC429_003636"
                     /pseudo
     CDS             complement(625..1125)
                     /locus_tag="PC429_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1151..2416)
                     /locus_tag="PC429_003637"
     CDS             complement(1151..2416)
                     /locus_tag="PC429_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF3849088.1"
                     /translation="MKQEPCDFSHGRFRLDTFKKSKTKIITPRKTYDLNDEFDEEYSE
                     FEAFMARKELKLISRRMQRGKIKSIEEGKFIAQAPPYGYDIKYLNNKERILVINEDKA
                     TVVKIIFDMYLKGTGAYKIAEYLNELGYRTTTGRTWYEKGVRDIIKNKVYCGYIVWNR
                     VERKRNSSRTRGINEQIESKGKHKPIISEETWNKAQSIRKKRSNSPTPNNKITNPLAG
                     IVKCGYCNYTLVGKFNTTSNGYEKYMVCTNCKNKSTKLNILEDKILEVLNKWLIDYKL
                     SLKEEKINFKLETEDNLEHILMNLNRELTNLEKQKERLHDFLEQGIYDSDTFIERSKV
                     LSSRIENIKKSINKTNIDIEIEKERNLAKTQIIPRIEKVLMEYTEEKEPCIKNVLLKT
                     VIDYAIYKKEPHQRGDDFSLIIYPNIPNN"
     gene            complement(2493..3611)
                     /locus_tag="PC429_003638"
     CDS             complement(2493..3611)
                     /locus_tag="PC429_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3849089.1"
                     /translation="MVVVEKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKKKAGFPKF
                     KSKKINRFSYKTNFTNGNIVYCGQHIKLPKLGMVKVRDKQVPKGRILNATISKEPSGR
                     YYVSLCCTNVDIEVFENTNNQIGLDLGIKEFCISSYGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNRKLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
BASE COUNT         1141 a          576 c          421 g         1524 t
ORIGIN      
        1 aaaatagtat tatacttata tttaaaaata aaagaatttt attcctattg tgtaaagtga
       61 gaatactatt ttcttatatt ggtataataa tagtaaatct ataatataga aaagaggtat
      121 ttctagtatg catgaaaata aaaaaaatct attagacgga agcttagctc aatataatga
      181 tacagcagtt ccaaaaatac cagtatttgc tggtaacgat ataacttcag aagtttatta
      241 ttttaaacct aatcaagttc ttaatgctca tagacaccct aatggtgaac aaatatttgt
      301 gttcctaaaa ggagaaggta aaatgaaact tggtgagcat gaatgtgatg taaaaaacgg
      361 agatactgta tttgtaccaa caggagagtg gcatgaaatt actaatggaa gcaatgaaga
      421 aatggtagcc gttcaaatta caaaaattaa tgctggagca gaatacagag gatagatata
      481 taagtacagt tagtacttta atatctattt tattatataa actttaatct caaatataaa
      541 aagagtcttt taaaaggctc tttttatatt tttgttaatt ttcattataa aaacaagaaa
      601 atatctagct aaaactaata tattttattt aatatttttt atagcattgt ttgcaaactc
      661 agtatttacg atatctttat aaggtgcttc cttatctagt tctttagctt ctttcataac
      721 agtcatcaat ttcttcaaac cttcctcttt taatacagga ttttgagacc atgcatctat
      781 tgatttatat ctatctacaa ctgttgttaa atcttctaaa gatatatctt caaagaaagg
      841 ttgcatagcc ttagctactt ctttgctaga tgcttgttga atccataatt gacctttata
      901 aacagcattt gtaaatcttt gaattaaatc tttattgttt ttcatgtatg atctcgtagt
      961 agagtatgct gtaaaaggta tttctccaga atcttttcct atagatgtaa ctatatatcc
     1021 actaccttct ttttccattg ctgttgctgt aggttcaaat gctgttgtaa agtcaccctc
     1081 tccacctgca aaagctccag ccattacacc aaattggtaa tcagtctatt tataacgtag
     1141 agttatacat ctaattatta ggaatattag gatatattat taaagaaaaa tcatcacctc
     1201 tttgatgagg ctctttttta tatatagcat aatcaattac agtctttaac aacacatttt
     1261 ttatacatgg ttctttttct tctgtatatt ccattaaaac tttttcaatt cgtggaatta
     1321 tttgtgtctt agcaagattt ctttcttttt ctatttcaat atctatattt gttttattta
     1381 tagatttttt aatattttct atacgagatg aaaggacctt tgaacgctct ataaatgtat
     1441 ctgaatcata aataccttgt tctaaaaagt cgtgtaatcg ttctttctgt ttttctaagt
     1501 tggtaagctc tctattcaaa ttcatcaaaa tatgttctag attatcctca gtttcaagtt
     1561 tgaaattaat tttttcttct tttaaagata gcttataatc tattagccat ttattcaaga
     1621 cttctaaaat tttgtcttct aatatattta atttagttga tttatttttg caattagtac
     1681 ataccatata tttttcatat ccatttgatg tagtattaaa tttcccaact agagtataat
     1741 tacaatatcc acattttaca attccagcca aagggttagt aatcttatta ttcggagtag
     1801 gggagttgct gcgctttttt cttatacttt gtgctttatt ccaagtttct tcacttatta
     1861 tcggtttatg tttacctttt gattctattt gctcattgat tcctctagtt ctactagaat
     1921 ttctttttct ttcaactcta ttccatacta tgtatccgca gtaaacttta ttttttataa
     1981 tatctcttac gcctttttca taccaagttc taccagttgt agttctatat ccaagttcat
     2041 ttaaatattc agctatttta tatgctccag ttccttttaa atacatatca aagattattt
     2101 taacaactgt tgctttatct tcatttatta caagtattct ttctttatta tttaaatatt
     2161 taatatcata tccataagga ggagcctgag ctataaattt accctcttca atacttttga
     2221 tttttcctct ttgcatacgt ctagatatta attttaattc cttacgagcc ataaaagctt
     2281 caaattcaga atactcctca tcaaactcat catttaaatc atacgttttt ctaggagtaa
     2341 taattttcgt cttagatttt ttaaatgtat ctaatctgaa cctcccatga ctaaagtcac
     2401 agggttcctg cttcatagaa ttttgcatac taaaatatgt cttactaatt ctccacaggc
     2461 catccccgta gttcctacgg ttcttacata cgttatttat tttgaattgt tattagtctt
     2521 aatccttctt ttaatatatt tatacttgca tttatatctc tatcgtgagt ttcattacaa
     2581 ctaggacaaa tccattctct tatatttaag ttctttactt cctcgttttt gtacccacat
     2641 ttattgcata tttgtgaact tgcaaagaat ttacctactt ttacaatttt tctaccatac
     2701 caattagcct tgtattctaa ttgacgaata aattctgacc atgatacatc agaaattaag
     2761 cgagatagtt tacgatttct aatcatattc tttacttgta aatcttctat gcaaataata
     2821 tcattctctt ttattaattt agtagatagt ttttgtagaa aatcatttct ttgattagct
     2881 atatgttctt gaagtcttgc aacttttaat cttgctttat ttctatttaa actaccgatt
     2941 gtctttctcg ataattctct ttgtaattta gcaagtttgt ttaatgactt cttaagatac
     3001 ttaggatttt ctataaattc tccataactt gagatacaaa attctttaat tcctaaatct
     3061 aaacctatct gattattagt attttcaaat acttcaatat ctacattagt acagcataat
     3121 gatacataat atctaccact tggttcttta gatatagtag cattaagtat tctcccttta
     3181 ggtacttgtt tatcccttac tttaaccata ccaagtttag gtaattttat atgttgacca
     3241 caatacacga tatttccatt tgtaaagtta gttttatatg aaaatctatt aattttcttt
     3301 gatttaaatt taggaaatcc tgcctttttt ttaaagaatt ttttatatgc attatctaaa
     3361 tcttttaagg cgttttgtag tgaaaatttg tcaggttctt taagccactt taattctttt
     3421 tttaagttgg tcaaatcaga actacactgt ttataagtaa aagtttcttt atcgttttta
     3481 taaacatcta ttcttttagc aagatattta ttatatacaa atctacaaca tccaaaagtt
     3541 ttgttaatta actcttgttg ttttttattt ggatatattc taaacttata agctttttcc
     3601 actactacca cctcacttta attgtttttt gatacacttt ttaatgattt tatcacattt
     3661 aa
//
LOCUS       DAMKMI010000228         3603 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMKMI010000228 DAMKMI010000000
VERSION     DAMKMI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.234"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     repeat_region   27..783
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=27..55
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(1442..3535)
                     /locus_tag="PC429_003639"
     CDS             complement(1442..3535)
                     /locus_tag="PC429_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF3849090.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         1261 a          571 c          350 g         1421 t
ORIGIN      
        1 aatttatgta aagcaacaaa agatggattt acattccata tagttaatat aaaacgattt
       61 caaatgatta gaataaaaat atcagaattg ctatttatat tccatatagt taatataaaa
      121 ctataaagtg ttacttttat ttattttatt ttttacataa tttacattcc atatagttaa
      181 tataaaacgc attatatggc tgtgtcattt tgcattctat tacatattta cattccatat
      241 agttaatata aaacctgctt aatgcagaat taagagagga agtgttataa gatttacatt
      301 ccatatagtt aatataaaac tggttgttcc tgctccggtg ttggtggaac tggtacattt
      361 acattccata tagttaatat aaaacgaata tgtatggata attttttact taacatatta
      421 gcatttacat tccatatagt taatataaaa cctgctgtaa tttctgatga agcttctcca
      481 ctaccaccat ttacattcca tatagttaat ataaaaccct tctttaactc ttatcgtatt
      541 taaacctctt ataacattta cattccatat agttaatata aaactttttg tgttaagaag
      601 aaaaaagaaa ttttgataat atttacattc catatagtta atataaaacc ttaaagaaaa
      661 aatggaggaa gattaagtga aaaaattatt tacattccat atagttaata taaaactgga
      721 tttaaataat caaaacaact ccggtttaaa aaacatttac attccatata gttaatataa
      781 aaccccaaaa taaacttagt attttcaaca cttacacgta tacaactctc ttaaatttgc
      841 agtgaacgag caatagtgca attgataacg cttatcatac actctcaacg ccttatattt
      901 caactgttac gccaaatttt ttcacaaata tcgctcactg caaaattcct ataattttat
      961 tatatcataa atatattact tttgaatatt gataccaatt tataccataa taaaagcaaa
     1021 aaaacgtaat ctatctacat tagatttaaa ttctataact taaagttttt tacataatat
     1081 ttatgcttgt ttaatcaatc taatatatca ttcttgtata ttgcttttaa tagtcttttt
     1141 tcttaaataa atatctatct gaacaagcat agtttaatta aaaacttttg tattccctta
     1201 aaactgtatc tacctcttat tattttattt atgatatatt atttttaatc taaaaataga
     1261 tatctatttt tattatggtt atcccttttt aaaatacttt aatcatatac tattcccaat
     1321 ttaacatcca ttgaaatgct attttcttat attgcaattt ataattgata tatcttcaat
     1381 ataataattt aatgtatttt tgattttaaa gcttttcagt gatattattg ataaattaga
     1441 attaattatc tagctttaaa aggtcaaaga tttctgttgc tgtctgcggt tttccaaata
     1501 aatatccttg tatgtaatct ggctttattt gtttaactaa atcatactct tcttgtgttt
     1561 ctacaccttc taaacatact tttattccta catcatgaca aattgcaaca ataaaatgta
     1621 taaatgtagc atcaaactta ctctttaata tatctttaac aaatactcta tcaattttca
     1681 ctatatctat tggagaaaat tttaaaactt ctagagacga gtatccagta ccaaaatcat
     1741 ccatagcaat ataaattcct aaatcttgta aagccttaaa tttaaactgt aaaatactca
     1801 tattttgtac tgtatgactt tctgtcaatt ctaaaacaag attttgataa ggaaaatttt
     1861 catcatctat aatttttact atatcttcaa taaatgtgtc ttctaatatt tgaacagctg
     1921 aaacgtttac actcacttta aaaaaaggat agtaatcaat ccatttccta aaagtacgta
     1981 atgccattcg aagcacccaa gcccctacaa tatttatcat atcattttct tctaatattg
     2041 gaataaattc caaaggagaa attgctttgc atttgtcatt agtccacctt aaaagaactt
     2101 ctacacctat tattttatga ctttctgtat ctacttgtgg ctggaatcga agactaaacc
     2161 ccctaaaatc atcattaatt gatgccttta aatcccgcat catttctaat gaatacattt
     2221 tattttttaa tatttcttgg gagaaaaaaa caagcctgtt tttaccatgc tctttcgcat
     2281 attgtaaaga gtaactagca cacttataaa gttcttttac tgtatctcca tgttttggat
     2341 atattacaca accagcagaa attgttatat ttaatccata ttttctccat agttgaaggt
     2401 gtataatcat attctgtatt tgattatata gagttagaat ttcattttct tcaacattat
     2461 caacaagaat gcccatttcg tccccatcaa gtttataaag ttcagcatta tctggtaaaa
     2521 tagattgtat agactgtgct agtgttttta ttatattatc tccaaaatcc ctatcataca
     2581 tctcattaac atttttaaaa tcgtcaatat ctaataatac tatgcataat ttttcaatca
     2641 taggatttga aattttatct tcaaatacac tcataaattc atgataattc agtaactgtg
     2701 tcaatgaatc aatcttattt tgtttaccta attgcgtcat tattcctgca aaaatacttg
     2761 gttctccaaa ttcatcacgc attagttgac ctctacatct taaccatata tattctccac
     2821 tacgattttt agccctaaat tccactgtat gataatccat ctggttttta cctatttcag
     2881 tgtttgactt ataaaaacga ttccagtctt ctggatgtac aatttttttc cagtaaacaa
     2941 gcggattttc aacaatttct cctggtaagt caaataattc aacttgtgat ggtgaatatc
     3001 gaaatacgcc tgttttcata ttgcaaatgt atatatattc atctgtacct ctaatcaaag
     3061 catcataaag caatgtagaa ttataatcaa aattgtctct tgaaaaatta aaaactcctt
     3121 tttcttcttt attatttgat atttcatgaa attcattaat cttaattggt ttatttaagc
     3181 ttgttgagtt tgcaatatct attgattgat gcatatgaac aactgtgcca tcttgccatg
     3241 taattaaact atcataattt tcaaaaacac gatttaactt attacatttt tcataccact
     3301 ttattaatac tcctttttta tcattcttta ataattctaa aactttacaa aaactacaag
     3361 tagaattttt ttcaggatat aatacttgcc aacaaacttt tccttctgga tctaaaatat
     3421 tatattcttc tttcattttt ttgttcataa aaataatttc attagtatga atatttgtaa
     3481 catatatatt aatctgtagt tgatttaata taacttcaaa attatgtttg ttcatatact
     3541 tatctcctcc atagttactg ctctttataa caacattgtt aaaattattc tttttagaag
     3601 att
//
LOCUS       DAMKMI010000229         3440 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMKMI010000229 DAMKMI010000000
VERSION     DAMKMI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.235"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(56..628)
                     /locus_tag="PC429_003640"
     CDS             complement(56..628)
                     /locus_tag="PC429_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF3849091.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(657..923)
                     /locus_tag="PC429_003641"
     CDS             complement(657..923)
                     /locus_tag="PC429_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF3849092.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(923..1132)
                     /locus_tag="PC429_003642"
     CDS             complement(923..1132)
                     /locus_tag="PC429_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF3849093.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1401..2261
                     /locus_tag="PC429_003643"
     CDS             1401..2261
                     /locus_tag="PC429_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF3849094.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2309..3337
                     /locus_tag="PC429_003644"
     CDS             2309..3337
                     /locus_tag="PC429_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF3849095.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDNEKIYINKCN
                     NLNVVNKI"
BASE COUNT         1351 a          384 c          552 g         1153 t
ORIGIN      
        1 aacacaacag taaattataa attatcaaat aataatagtt atacaaataa aaattttaga
       61 acattttttg agattcttct aactcgtaag catccattaa ggcttcacca aatctttgat
      121 aatgaattat ttctctttga cccaagaatt ttaatacatc tataacatca acgtcatctg
      181 ttaggtttat aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat
      241 gtaaatcggt tacgggattt gacataactg ctataggtct tacatcaaat ggaacaccat
      301 ttgaatctgt tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag
      361 cttttaactc ttctgggctt gcatcagaag ttaactgata aaccatagta catataagct
      421 caacgtgagc tagttcttct gtaccaatat ctgttaaaag tgcacgcata ttaccagtag
      481 gcattgtata tctttggctt aaatatctta aagatgcagc aagttctcca tttggcccac
      541 caaattgagt tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt
      601 gttgtatagt tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa
      661 taattaaatt ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt
      721 ggagcatacc caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc
      781 gcaaattgat tacataatga attataagtg ttgatagcat ttttatcatc agggtgattg
      841 tctaaatata agttcatatc aacacatgca aaagatgttt cctgaacttt tctcataagt
      901 tcagctctag atgagtcttt cattatcttc tacctcctct atattttttt ggcttagcat
      961 ataattcttt gttgtacatt tcagactcta gtaagtttag ttctggaaat atagttccaa
     1021 attttaatgc atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc
     1081 tagccaaaga tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa
     1141 cctccttgtt gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa
     1201 atcgttacta ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg
     1261 ttatttatgt tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta
     1321 aagtaaaaat tatagtattt tgtggatgaa ggtagacttt tttgtaaaat attattaaca
     1381 taaatatacg aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc
     1441 cttttgagga aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagga
     1501 aaaatttatg gtctaagttt atgaaggcaa taagagatta taaattggtt gaagaaggtg
     1561 ataagatagc tgtagcgatt tctggtggaa aagacagtat tcttatggct aaaatgtttc
     1621 aagagttaaa aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg
     1681 gatatcatgc aaatataaga caattactta tagataattg tgagtatcta aatataccaa
     1741 tacatttatt tgattcaagg atatttgaga tagcagatga aatagcaaaa gattatccat
     1801 gctatatgtg tgcaagaatg aggagaggag cattatattc aaaggcagaa gaattagggt
     1861 gtaacaaact tgcattaggt catcattatg acgatgtaat agagacaaca atgcttaatt
     1921 tattgtgtgc tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag
     1981 gtataaaaat tataagacca ctttattata taagagaaga acatattata agatttattc
     2041 aaaatagtgg tatatggcca ttaaattgtg catgtatggt tgctgctaaa aaaacaggta
     2101 ataaaagata tgagataaaa gacttaataa agagcttaga atcaaatttt aaaaatgtag
     2161 aaaaatctat atttaaggcg gcagaaaatg taaatcttga ttcagtactt ggttggcaaa
     2221 aagatgggga gaaacattct ttcttagaaa attttgaata aaaatagtta aaaagaatca
     2281 ctataaacaa ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa
     2341 tatgccaaat tctaaatcaa aaagagcagt atttaattct gatataattg caatcggagc
     2401 agttatgtat gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt
     2461 aagtaatgag gagttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt
     2521 gaagagtgca ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt
     2581 ttctgatatt aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac
     2641 agatatgaga agtgctaaaa aaaataacca tccaaggttt gtaaataaca ttagagattt
     2701 atttgtagat ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc
     2761 attgaaaaat ttacttgaat ttgttaactt agagtttagt ggagatgccc atgacccatt
     2821 aaatgatgca tataatttat ttattttgga tgaggcaatc acaaataatg tagatataca
     2881 aaatctttta ataatacgtg acataataag accaccattt aacgatataa attctaattt
     2941 agataattgt tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat
     3001 tgtagatttt tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga
     3061 tgtaaatata caaaatttag aaattatgaa agatataagt aaaaaaatgg atactataga
     3121 taaattgaaa gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgatat
     3181 gaaagatgta ctagaagatt taatgcttta tagaatgaat gaagatgagt acaagtatga
     3241 gataaaaaat ataataaaaa tgtttgatga agacttagat aatgaaaaaa tatatataaa
     3301 taaatgtaat aacttaaatg ttgttaataa gatataagaa attataaata atagagtaga
     3361 acttgacaat tataacaatt ttttataaaa ctgaatcaag ataaaattaa attaatataa
     3421 aaataatata caataaaaaa
//
LOCUS       DAMKMI010000230         3438 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMKMI010000230 DAMKMI010000000
VERSION     DAMKMI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.236"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            55..1065
                     /locus_tag="PC429_003645"
     CDS             55..1065
                     /locus_tag="PC429_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF3849096.1"
                     /translation="MVYFVSIELKDRSLVNNQYKGKIKSANLSVDYEINQVMKDIDKL
                     GLNTVNVPIIINVESINSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENGKL
                     YETDWNPINKKRFFYTWQNAILNELIIDVANPLDVNVLNIGSNFVHLEEYQQNWGEII
                     DFVQSKFDGFVTYRTNWWYTKKDDLSSKLFYEQKLNNNFFDKLDFISIAAYFELSNKP
                     VNTVDELISALHSSTVNNRGQNIKQEIYNLYKAHRKPIFFGELGFSNRESASSQPWNH
                     TPSKAVNGEEQARCFEAYKKVFENEDWINGFSVFCVGKIDDEKNFYPSKESIKVIKSW
                     YE"
     gene            1119..2243
                     /gene="wecB"
                     /locus_tag="PC429_003646"
     CDS             1119..2243
                     /gene="wecB"
                     /locus_tag="PC429_003646"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF3849097.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGMQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2321..3295
                     /locus_tag="PC429_003647"
     CDS             2321..3295
                     /locus_tag="PC429_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF3849098.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT         1438 a          336 c          597 g         1067 t
ORIGIN      
        1 tttaaattaa aaagaagtat tttaatctca ttaataatct taatttttat atttatagtt
       61 tactttgtaa gtattgagtt aaaagatagg agtctagtaa ataatcaata taaaggtaag
      121 attaaatcgg ccaatttatc tgtagattat gaaataaatc aagttatgaa ggatatagat
      181 aaattgggat tgaatacagt aaatgtacct ataattataa atgtagagtc cattaattct
      241 gatattatgt caatagataa taatagtaaa gaaaaggcaa ttaagttaat aaaaaaatta
      301 aataaaaaag gtatttcaac aatattagag gcatatccat ggattgaaaa tggaaaattg
      361 tatgagactg actggaaccc tataaataaa aaaagatttt tttatacttg gcaaaatgcc
      421 atattaaatg aacttataat tgatgtagca aatccactgg atgtaaatgt tttaaatatt
      481 ggttctaatt ttgtacattt ggaagaatat caacaaaatt ggggtgaaat aatagatttt
      541 gtacaatcca aatttgacgg ttttgtaacg tacagaacta attggtggta tacaaaaaaa
      601 gatgatttaa gtagtaagtt gttttatgaa caaaagctga acaataattt ttttgataag
      661 ttagatttta tatctatagc agcatatttt gagttaagta ataaacctgt taatacagtg
      721 gatgaattaa tttctgcctt acactcatct actgtaaaca atagaggaca aaatataaaa
      781 caagaaatat ataaccttta taaagctcat agaaaaccta tattttttgg agaattaggt
      841 ttttcaaata gagaaagtgc aagttctcag ccgtggaatc atactccttc aaaagctgta
      901 aatggcgaag aacaagcaag gtgttttgag gcatataaga aagtttttga aaatgaagac
      961 tggataaatg gtttttctgt gttttgtgta ggtaaaatag atgacgaaaa aaatttttac
     1021 ccatcaaaag agagtataaa agttattaag tcttggtatg aataattttg aaagtgtaga
     1081 atttatatag attaaataga tgattaggag agacataaat gaatgacatc aaggttatga
     1141 ctgtatttgg aactagacct gaagctatca aagtagcacc tttaataaag gaacttgaaa
     1201 aaagagagaa tataaaaagt attgtttgcg ttacagctca acatagagaa atgcttgacc
     1261 aagttataga aacctttaat ataaatgtag actatgattt ggatataatg gaaaaaggac
     1321 aaagcctgaa tgatataaca tgtaagattt taaataaact tcccttgatt ttaaacaagg
     1381 aaaatccaaa tataatattg gtacatggag atacaaccac aactctagct acaagtttga
     1441 ctgcatttta taataaaact cttgttggtc atatagaagc tgggcttaga acctatgata
     1501 aatattctcc ttttccagag gagttaaata gacaacttac aggtattatt gctgatatgc
     1561 actttgctcc tactaatttg gctagaaaaa acttgatttc tgaaggaaaa cctaataata
     1621 atatatttgt aactggtaat actgctattg atgctttaaa gatgacaata aaagaaaact
     1681 ataatcatcc tataattgat gagattggta acgataggat gattttacta acctctcaca
     1741 gaagagaaaa tttgggtaaa cctatgaaaa atatatttag agctataaag cgaatagtag
     1801 atgactttga agatgtacag atagtatatc ccatacattt aaatcctaaa atacgaacta
     1861 tagctgatga gatatttgga aaatttcctg aaaaaattca tattatagag cctttagatg
     1921 tagctgattt tcataacttt ttaaataaat cttatatgat aatgactgat agtggaggaa
     1981 tgcaggaaga agctccatca ttaggtaagc cagtccttgt gttgcgtgat aaaacagaaa
     2041 gaacagaagg aattgaagca aagacactta aattagtagg tactaatgaa gatagaatat
     2101 ataattcagt gagcgattta ttaataaata aagataacta tgttcagatg agtaaagcat
     2161 ctaatccata tggagatgga aatgcaagca aatatatagt ggatataata attaaaaaat
     2221 ttaattgcaa atatttaaat tagataagac ctaaaacatt aactaattga tataagattt
     2281 atgataatat taaggaatac aatttttaag ggggagtaat atggctagaa aattaataaa
     2341 aaatttgggt aaaagtaaaa gtgtaaagag agtgaaactt ttatttaaga agatttttat
     2401 tactgtattc atagtagcaa gtatagttgc tatttttaat ataacgaaat attttgagga
     2461 attatacaaa gtaagggatt taaaaagtac taaaattgaa tactatatgg atgtggctga
     2521 tgaagcagga gatggaaagg tccaattaag ttggaaggcc ttacttgcta tagacatggt
     2581 aatacatgat gaagatttaa gtaatataaa aaagaaagac acattggata taggggaaaa
     2641 gtttatagta gaagataaaa atgataaagg cgaaaaggtg tataaggtaa aaaagtttaa
     2701 taaggtatta agcgaattga aatttgactc ttctcaaaaa agtagagcaa gaaaatacat
     2761 gaaagattta gaatacacat accttggaaa taaacaatta gatagtagtg atgaaaaaat
     2821 taaatttata aagaagttag aagactcagc tataagagaa tatattgatt atggaatatt
     2881 gccctctata acaattggac aagctatatt agaatctggt tggggaaatt ctaaacttac
     2941 aaaacagagt aataatttat ttggtataaa agcagataaa gcatggaaag gaaaaagtgt
     3001 agaaatttca acttcagagc attataatga aaaaattgta gctagtttta ggtcgtataa
     3061 ttcattacaa gattctgtca aagaccacag tttattttta attaataata aaagatatag
     3121 aaaacatgga ttgtttgagg caaaagatta tattagtcaa gctcaatcat tagaaaatgc
     3181 aggatatagt acagcagaag ataaaaaagg aaatcgtata tatgcagagc tattaataga
     3241 tgttattagg agttataatt tgcaattgat agataataaa gttgagacaa aataattgtt
     3301 aactttatat gaggagataa caagataaga ttcttttcta caaaaagaat agaaaaattg
     3361 taaaaaaaac taaaaaaatg tattgacttg ttatttaaaa ggtgatatta ttattactgt
     3421 actcgaaaga gaacaaaa
//
LOCUS       DAMKMI010000231         3372 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMKMI010000231 DAMKMI010000000
VERSION     DAMKMI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.237"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..2062)
                     /locus_tag="PC429_003648"
     CDS             complement(<1..2062)
                     /locus_tag="PC429_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF3849099.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSMKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYI"
     gene            complement(2085..3341)
                     /locus_tag="PC429_003649"
     CDS             complement(2085..3341)
                     /locus_tag="PC429_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF3849100.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
BASE COUNT         1134 a          536 c          311 g         1391 t
ORIGIN      
        1 ttatatatag cattgataat atcatgacta caattatagt aataaaagaa atcattaaaa
       61 atactttagt tgtcttattt atactcatat tccaacttga atcttttact tctttcttag
      121 attttaaatt ataagctaaa tcttctacct ctccgtattc atcaattatc aatgtatctc
      181 cttttaaatc attaacttta cttttattca acaaataatc cattcctaaa tataagtttt
      241 tttcattcat tgaactaatt accatgatat ttttttgatt attatatggt gattttatta
      301 attgaatggt agaaatattt tttccataat catcaataaa gcttatctta tcatttgata
      361 taaaattgga gtaattttta tcgaatttta tatttaaatt attatttaac atctttataa
      421 cagaattatt atgtggcact ccaaaaacta caatattagt atccttatac ttgtcactaa
      481 attcttttcc tctaattaca tttatatttc cattatgtga atttatattc gcacctaaag
      541 taacacctaa tctaaacatc caagtcatag cttgtgaccc tgaataatct ggcattatca
      601 cagttaaatc attaaattca tcatctctaa caaatggata cggataattt tcaaaagata
      661 aggtttcatt ttctttcata gataacgcca aataagaatt atttgataca tatgcccaag
      721 gattattact ctctctagta acacaattcg aattttttat actcaaatta aatgtcaatt
      781 taacttgata atagttttta ttatctatat ctttaggtat ttttaattct aacttatcat
      841 tattagaatg tgacctatcc aattttttac ttcccacaat aacatcattt atagacactg
      901 taactaaaga tttttcaaaa tctaaattgt cagaatatct taaatttaag ataatctttg
      961 aaccatcatc taatacctta cccgttggta ttttgacatc aaacaaagct tgttgattaa
     1021 atgctccttc caacaaaaag tcagaatatc ctaagtcttt taatgttaaa tgacctaaat
     1081 ttaatttttg ttccctatta atttttatat tagtctcttt gtttacaagt actgatgatg
     1141 ataaaacttg gttagaaagt ctattttcta ttaataaatt tgatgccttt attaaatcat
     1201 cttcattact accaattacc accatcatct tcttattctt attgtatggt gaatctactt
     1261 gcttgattat acagtttgaa gataataggg tttgttcctt tatacttaaa atatccaaaa
     1321 tttcttctgc tgtattttca ggtttaccaa tatatataat attatcatct gaccaatttt
     1381 tcatttctga atataattta acatctaact ttaaatcatc attttttgtt atttttccaa
     1441 atgctgatgc caaattaaac actgctgtag tctctcctct agtcatatta tctggtacta
     1501 ctatagttgt atctaattta tgattatttt caatttctgc atatggatac ggatattcat
     1561 ttattgagtt ttccaccttt ttctgcttat atctaattga tatatatgat tctttgtgaa
     1621 taaccatcca gttcccagta ttggaatcat cttggcaaat tttatcagat attgttttgt
     1681 atgtttttat ttttatttca ttatatcctt gaattatata atcttttgga actaatactt
     1741 ctaatgtatt tttataattt gtcttagcat ttaatttaat agattttatt ggtacattat
     1801 ttatcaatac agttaaactt gaaacatctc catttaatat ttggctctta ctaaaattaa
     1861 gatgcaataa aacttcttca atatcccaat ttttatttac ttcaaaaaaa gtactattac
     1921 ttcctataac accatcaata gttatatctc tttcaaactt ataattttta actttagtct
     1981 catcagcatt tactttgtat ataagtgata tatttgaaaa aaacaatact aaagatataa
     2041 ttgatataat aaattttttc atgttaattc ctcctgtaca tgaatcaaaa tctttctgtt
     2101 ttataccatt taacctctct tttaaaaatt atatctttta gatactctat cattcctttt
     2161 atagctacaa acagccatag ttgagagtat gtaaaataca ttattgccac tatgaatata
     2221 ttttcaattg ttgcttcacc tttttctata gtaaggctta tactcacttc tataataaat
     2281 aaaagataag atagtatcca tattagaaaa aagttaattg gaatagaaat ttctactagt
     2341 ttagatatac ttaaaacaaa taaaatatcg gatataatta cagatgttaa aaatagaaaa
     2401 tatacagaaa aaaagtaagc tatatcaaac acaatcttat ttcttccctg tttaaatata
     2461 ttttttatat atttcattaa tacatatata ttcccttttg cccatctagt acgttgcttt
     2521 atccacacct taacagtttc tggctcttgt tcccatgtta tagattgtgg tacaagcttt
     2581 atcttgtatc ccaatttata tatcctaaaa cttatttctg tatcttctgc aattgcttta
     2641 ctatcccatc ctccaatttc ttctatgata cttctcctca atataaagtt agttcctggt
     2701 attgtacata gattaaaaag ttgccatcta ccagcttgtg acatccattg aaagcttaat
     2761 gtttctatgt ttataaattt ggttaatagg tttttatttt tatttcgagt cctaaatttc
     2821 ccaataacag cacctaattc atcattcatt actatagttt gtactagata tctaagtgca
     2881 tttttatctg gtgtgttatc tgcatcataa acagctataa aatctccttt tgaaatcgta
     2941 tatccaatat ttaatgcatt agactttcct tttccacctg ttaaactatc tgtatttatt
     3001 atagtgaaat tataattgtt gtacctatcc tttatatttt ctaaaatttc tttactatta
     3061 tcagaagaat tgtcatttat aacaataagt tccatcttac tcttaggata attgagtaat
     3121 agcaaagact ccacagttct acctatcact ttagcttcat tatgagcagg aacaagtatt
     3181 gatatcatag gatattcatc aatttctttt atttctttat caaagttttt aaaataaaat
     3241 atatatccac ccatagctaa aataatattt ataagcaata aagaccatat actaaacaga
     3301 ctaaatacga acaaatactc tgatacattt aaatctctca taaatttctc ctaaaatatt
     3361 ttaaaatatt ga
//
LOCUS       DAMKMI010000232         3355 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMKMI010000232 DAMKMI010000000
VERSION     DAMKMI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.238"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..933)
                     /locus_tag="PC429_003650"
     CDS             complement(<1..933)
                     /locus_tag="PC429_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF3849101.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNTL"
     gene            complement(973..1320)
                     /locus_tag="PC429_003651"
     CDS             complement(973..1320)
                     /locus_tag="PC429_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849102.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(1538..2494)
                     /locus_tag="PC429_003652"
     CDS             complement(1538..2494)
                     /locus_tag="PC429_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF3849103.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2937..3305
                     /locus_tag="PC429_003653"
     CDS             2937..3305
                     /locus_tag="PC429_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF3849104.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         1210 a          443 c          420 g         1282 t
ORIGIN      
        1 taaagtgtta cttttattta ttttaataac agattcaaca aaactctttt tatctcctaa
       61 ttttaagata aaatcaggat attcttcaaa ttcattgagt tttattaatt tgaaagaaga
      121 agatagattt ttaaagttaa taattttaga gttattcact aatacacttc tttttttaaa
      181 ataaatttct tttttactta tatttagtat agttaatatt tgtttaagat atattattat
      241 atcttctgga gttctatcaa atttgagttc aaaattttca tcttcaaagt agggaatata
      301 atataaaggt gttttgagag caggtaattt attatataat tcttcaagaa tatcgatatt
      361 tttaggggag ttaaagaaaa agcatttaca tacattcatt aaatccacag catctgaaag
      421 tgagttatgt ttaatatctc cttctacaga aaaaattttt ttcatattca gtaaagatat
      481 ttgcttagat aagattctat tattgtatgt aatatgttga gatataaaag gttgaatgtc
      541 aatcatcata ttaaataact ctaagcattt acctgtatat ttatacactc taaatgaagt
      601 tagaaggcca gttatatcaa aatcacccca attatatatt gtaacatcat caaatttaca
      661 aaaccattta taaaaatcat ccataacata taaaagatta ggagcattat tgatatctag
      721 ttggttcaat ttggttaaat ttttacattt atttgaaagt attttattgt attttggctt
      781 aactaaagag taataattat ctattgtatt aaattcgtta tcaactaaaa ctccaccaat
      841 agaaattaat tccgcaccat tatattcttt tggtttattt ttattatcat ctagtatatt
      901 aaattcaaag tctataaata acttgtattt cataaatcta aagcacatcc tcaggtaaat
      961 tatttattga gcctaagcat cataagaatc aatagatata atgactctaa acatcaaagg
     1021 ttcattacaa cttttattta tttcataatt taattcaaat aaatctttta agatataatt
     1081 tataagtatt ctttcccaac ccatttttaa attaacttta gagtctatta atatattttc
     1141 tatcttttta aatatttcta tatttgctgg tattatactg agctcaatat tacttttaaa
     1201 cgggttagac tgtaaatctg ttataagtat attttttaat tcctctgtaa gttcagaaat
     1261 taaactatca cctatgtatt tttttatgat ttcctctcta atttctttta aatttagcaa
     1321 tattagtacc tgctacttac atagtagctt ttcctttcct tttaatacta ttattcattt
     1381 ttgaatctta tttagatata ttttaagatt aaaagtcttt agctatataa ttttttaccc
     1441 atttttaact tatattaaca tattacaggc ttatatctcc accctgttta cattgaggat
     1501 ggagtatatt gcctattttg ataaaaatga taggttgcta tttgcctatt cctttttctg
     1561 ataatttttc taacatatct ttggtcattt tatctaaatc ataactaaac ttaaagttcc
     1621 attcttttac tgcactacta ctgtctagag agttaggcca agaatcagct atactttgtc
     1681 ttacagggtc tacatcatat tctatcacaa aatcaggaat atattttttt atagaatcag
     1741 ctatttcttc aggagaaaag ctcatagcag taatattaaa agcatttcta tggatgagtt
     1801 tatcagctgg agcttcgatt aaatccacta ttgattgaag tgcatcaggc atatacatca
     1861 tatccatttt tgtaccttct gctatgtaag atttatatct cttatttttt aatgcctcat
     1921 aatatatatc aactgcataa tctgtagttc ctccaccagg aggcgtaaca taagaaataa
     1981 gacctggaaa tctaacacct ctagtgtcta ctccaaattt tgagtgataa taatcacata
     2041 gtaattctcc agaaacttta gtcacaccat acatagtatt aggtctttgt aatgtatctt
     2101 gaggagtcat attttttgga gaattttcac caaaagcagc aattgaacta ggtgtaaaaa
     2161 atttacaatc caattctttg gctatttcaa gaccatttat taatccattc atatttaagt
     2221 tccaagcttc tagaggttta ctttcagcaa ctgcagaaag taaagatgct agatgaatta
     2281 tagtatctac atcatatttt tttgcaattt caaaaaaacg attcttatct gtaacatcta
     2341 atatctcaaa tataccagat tcacatactg gattaccttc ttttactctc cttgaactag
     2401 cgattacatt ttgttctcca tactcatttc taagctttat tgttaattct gaacctattt
     2461 gtcctaatgc acctgttata agtatttttt tcaattttac aacctcctaa gggtttaatt
     2521 tatgtagtac tacttatttt tattttagca aatcttttgt ttaaatacaa agaattaaag
     2581 ataataatat acgacttaat tgaatgaagt gcagtaagtg cgtaaaaatt taaagctaat
     2641 atataatggt ctattgtata acaataaaag agtagattaa attattatat atagattgtg
     2701 tgactaagtc acagaaaata gagctaattt aatatataat aaaagcatgt caagtaaagc
     2761 tttaaattaa agtaacttta tataaaaagt aatataaatt gataaaatta taaatttatt
     2821 tatagtgaat ataggttgga ttttatagat acaaaaatag tatatatgat atgtagacca
     2881 ttagcatgtt taaagtagca aaacatataa aataaaattt gaaaggaagt ataaaaatga
     2941 caagaccatc aatatatcat agccaaggat acacttgtgc agaggcatta ataaagtctt
     3001 ataatgagga acacaacaca gatataccaa tctcaatagg aagtggtatg ggggttggaa
     3061 tgaatgttgg aagtgtttgt ggagctgtaa atgctgctgt tgttataata gggtatttaa
     3121 atgggcgaaa tagtaattta gatgaaaata tagcaaggaa gtattcaaga gagctattga
     3181 atagagtaag aaataaatac agctcagaaa tatgtgtatc tcttaaaaaa aataaagtaa
     3241 cttgtgaaga aataataaat ttcacatatg atgctctaaa tgatatgcta aaaaatcaaa
     3301 aataattcta agaaacaagt attttacatt cgatagaata cttgtttttt tatga
//
LOCUS       DAMKMI010000233         3320 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMKMI010000233 DAMKMI010000000
VERSION     DAMKMI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.239"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(39..404)
                     /locus_tag="PC429_003654"
                     /pseudo
     CDS             complement(39..404)
                     /locus_tag="PC429_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(425..1504)
                     /locus_tag="PC429_003655"
     CDS             complement(425..1504)
                     /locus_tag="PC429_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF3849105.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1558..2847)
                     /locus_tag="PC429_003656"
     CDS             complement(1558..2847)
                     /locus_tag="PC429_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF3849106.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(3010..3221)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1096 a          665 c          367 g         1192 t
ORIGIN      
        1 tcatgactaa agtcacgagt gtgcgtagct attttttaat caaactcata tccaccagat
       61 accaatattg aatctaattc tattttttct cttaagtctt ctggccaatc tataaatgga
      121 agaatggcag tagtggctgg ctttcctatt cttatcatag ccacattatt aagtcttttt
      181 ctttctacaa tagcatttgt acaatgagtt gtaccaagca tagcatattt tattaatgaa
      241 tggtcaatct tactttcatc tataactttt tttaaggcat tgtaaacacc agtttcaaca
      301 tcttttgtag ttgccacctt aactgaatga attactttta attccccatc cagtatacaa
      361 gcatctgtat ttgttcctcc aacgtcaatt cctacacgat acatcaaaat ttcctcctaa
      421 acttttattt tccttgtaat tcttctactg ttttatattc cacatcatat ccaaagtatc
      481 caggtccaac tgtctcaatt cctttttctg ttctccattt ttcattacaa ggaaaagcta
      541 ctaccataac acgagagcca tattttattc tctctgtagt tattggaaat ccagtttcta
      601 aatctaatac tgctattaaa tctggtgtaa tacaaagtgg tgtatcacct tttttggcaa
      661 ttaaattctc attttggaat aatatagaat actctccatc atactcatta atcccagcaa
      721 aaaaggcatg tcctctagta aatccacctt tcaactctct cttaatatca attacttttc
      781 cttcaaataa cttgtatcca cgcaattctt ttagtatatt ctcaacagca ccttttgaat
      841 cagatttatt ttcaagtaaa aaccctccta tagtttcagc tattgtaaga gtattaccta
      901 tagcagcttt tttaacttga gctccactta aattatagtc actaatagaa gaacttcctc
      961 ccatttctac agttaaagtt ctagctaatc tttctgacca aattccatcc acaggataaa
     1021 ggatggcaga attacctttt tcatcagaca ttacggcagg agatgcctca tatccttcaa
     1081 gataaaaagt taccatttga gcttcaggga aagctctacc catagaatct gcatctaaga
     1141 taggaagtcc ttttttagca gcaactgcta taggcataag tgagtttatc cctcctattt
     1201 ctataggaat tataacatcc acttttctat ttaataattt ctccattatt tctaaagggg
     1261 tagttaactc aacttctgaa agtaattttt ctattgtaat tacaggagaa cccataccag
     1321 atactggaac taccaaagca tcatcatcaa gttcatctaa acttattact tttacaggac
     1381 catacttttt aatggcttgt tttgcaacca atttacctac atatgggtca cctccacctc
     1441 cagttccaag cactgctgca ccaactgcta ttttatctac actttcttct gttaaaaatc
     1501 tcatgttact ttctcctcat atttctaatt tattttatac tttataaaca aatttcttta
     1561 tactgctttg tctatttctt gcttattttt tgttgaacaa agcacttttc caataataga
     1621 ttgtactaca aaccctgcta taaaaccatc taaaggtgct atagtagtaa gagttataaa
     1681 tccatatgtt gaaaggtaag ttattaatat accaattatc caagacacta ttgaacgttt
     1741 aactattggt ttatataata ctcctttatc aaagccttta agttcttgct ttactatgta
     1801 atattctgct gtatatacac caccaacagg tgcaattaca attccaagta aatttaaaaa
     1861 tcctataaaa ccttcatata ttccaaatac agccaataat gttgctataa taccaaggac
     1921 tatagttaat actttctcag gagccttctt aagtacaaga gctatactta atgatgctga
     1981 atatacatta cttgtatttg tagtccattg tgctaatgta agtactatta tacctggtat
     2041 agcaattcct aatgtaataa aaattctcat aatatctgaa gttcccatac attttactaa
     2101 tactatcgaa actattatca taaaagcatt tccaaattgt attcccaaaa agcttgaaag
     2161 agcacagtct cttctacttt tagcccaacg acttatatca ggagatacaa ttgcaccaac
     2221 tattattata ctcattgaca gagaaactcc acctgcaaat gtcatagtag atttcatatt
     2281 atcatcaact gaaattccat ttgctctaaa agctaagaat atagttagca tcataagaac
     2341 taataaaaat ggtactgaat acacactcaa cttctccatt gccttatatc cataaattgc
     2401 agtactcatc atcaagagac cacctattag gcttaaaatc tgcatactta catctaaatt
     2461 aaatatatct tttattatta tctgtgcatt ttgagcaaag aaacctactt gaactccaaa
     2521 ccaacctaat agagaaaccc ctataactag agaaaatatt tttgctccag cttctccaaa
     2581 tacatacttt gttatcatag atgtagataa acttgtagat gagccaacca aagagcatgt
     2641 agtatacaaa attgcagaaa aaaatgaacc aacaattact gacaatattg cttgtttcat
     2701 tggcatactc tgtgccaact cagcgcctaa aataacagat gataaatcca cacctattgc
     2761 tactaaaact gaaaacatag ctacccaacc acgtttttga ttctctggaa tctgttccag
     2821 agcatagtta attttttctt cttccatttt catccctcca tttaaatcgt aaaactactg
     2881 accttagcat attttacaat tttctaattt ataaatttat taactatcat atttttataa
     2941 ttttcattta atatatagtt ttaaattttc tgaaataagt ttataataaa aaacgcctct
     3001 tggaaaaatc caagagacga atataaatcc gtgttaccac tcttattagt aaaaattaat
     3061 tttactcact cattttctta aggcgaatta aaccactatt ctaacataat tacaatagta
     3121 ttctccaaag tccttttcac ataaattcca atattagttc tcagccaaat ctaactctct
     3181 gtaaaattcc tttatgctac tttcttcttc tacgaatata taatattgat ttgtagttaa
     3241 tttatgattt atatcataat atatatgcaa tattttgtca acttaaaaag tcaattactt
     3301 gcatttttta gttttatatg
//
LOCUS       DAMKMI010000234         3313 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMKMI010000234 DAMKMI010000000
VERSION     DAMKMI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.240"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(273..530)
                     /locus_tag="PC429_003657"
     CDS             complement(273..530)
                     /locus_tag="PC429_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3849107.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENNV"
     gene            1110..1331
                     /locus_tag="PC429_003658"
     CDS             1110..1331
                     /locus_tag="PC429_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF3849108.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            2149..2379
                     /locus_tag="PC429_003659"
     CDS             2149..2379
                     /locus_tag="PC429_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF3849109.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            2392..2541
                     /locus_tag="PC429_003660"
     CDS             2392..2541
                     /locus_tag="PC429_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF3849110.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            2798..3235
                     /locus_tag="PC429_003661"
     CDS             2798..3235
                     /locus_tag="PC429_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDF3849111.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLKNNEKVIINRRYIKNIKEKLNI"
BASE COUNT         1348 a          420 c          352 g         1193 t
ORIGIN      
        1 atagtttatt tatttatttt tcccctttat acaagcaatt gtatcatttt atctattttt
       61 tagccaacaa atattttcga ccattttctt tgtattctca atatatctcc agtatttaac
      121 aaattttttt atagaatata caatgtattt aaattaataa gtagtaaatt tagttaattt
      181 gtctaaaaaa actttataaa acatcttctt tgtctttact cttacgttta acaaggctat
      241 tttcattatg tttaaacatt attttattat atttaaacat tattttcatt gagtattcgc
      301 ttaatattgc ttacacacat attatatcta atagccaatt cattgtggtt gactccatta
      361 aattgtttta ctatatccct atttctagca tatctaaaaa gccccttata cgtaggtata
      421 taagtatttg ttcctccata caacttggca acctctataa acttatcaat tccaattatt
      481 tctaccattg catgaaattg aactggtata tcctccactt ttatcttcat aagttacacc
      541 tcctttaaga tatttttata gacttaacaa agtttttatt taatacatta ttgactaaac
      601 tatatttatt agttttattg aatctaataa aaaatttctt agctttaaaa ttaatttttg
      661 tattaacttt aataaaattt ctcataactt ataactttgt tagctttaat aaagatttta
      721 atggtttaaa agtaaaatgt taatattgga cttttaactt ttttatttac agattaaatt
      781 taataaaatt tttaacataa gctttaacat ttaatttaac ataaacttta gtacctaaca
      841 tttaactcta cacatatgaa aatccataca aaactatctc aactaagcat gaatatatac
      901 aaatactatt tcaaaacact aagaacatat cttcacaaat aacttgtaaa ctttttcatc
      961 agcaaaaaag taaaattttt cctataaaaa tttacacaaa aaatctttag aaattactgt
     1021 ttaactccga tattatctta gatgatagta ttatagctaa caaagaaagt cgacaaatct
     1081 ttgaaattaa atttttagga ggtaatttta tggaagttgt ttcaacaaaa aatttatcta
     1141 gtataaaact aaaattggat gctggttttg atgataaggg taaggcagtt gttaaaagta
     1201 aatcatttgc aaatgtaaaa gcagaggcat taaatgaaga tgtttatgct gttgcagaag
     1261 caatagcaag tcttcaagag aaccctattg tagatatttt aaaattagat agtacatcat
     1321 tatctaaata gtacctttta aacgtactct gctttacaaa ttaaaaaaat taactcttta
     1381 tataaaagat taagttatat aacgcactta atacgctata gatttggcag attataactt
     1441 caattttgat tataacttta atatattaat gctttgaaaa attaatatat taaacgtatt
     1501 tcaacttaaa catgttagct attttagatg ctaccctact ttaatctaat taattgaaat
     1561 aaattaaata ttttctatat cagcaataac tagagtcaac aattaaaata ctaatattga
     1621 caattgaaac actgatacta acaagcaaaa cactaatatt gacaattgaa atattaatac
     1681 taacaagcaa aacactaata ttgacaattg aaatactaat actaacaagc aaaacattaa
     1741 tattgacaat tgaaatacta atactaacaa gcaaaacact aatattgaca attgaaatac
     1801 taatactaac aagcaaaaca ctaatattga caattgaaat actaatacta acaagcaaga
     1861 cactaatatt gacaattgaa atattaatac taacaagcaa aacactaata ttgacaattg
     1921 aaacactgat actaacaagc aaaacattaa tattgacaat tgaaatatta atactaacaa
     1981 gcaagacact aatattgaca attgaaatat taatactaac aagcaaaaca ttaatattga
     2041 caattgaaat attaatacta acaagcaaaa cattaatatt gacaattaaa ataccaatat
     2101 taacaagcaa acaattaaaa tataaattat tatatcggag gtaattatat ggaaattaag
     2161 aaaaaattag tcatgagctt taaaaatgta aaggataaac aagtttcttt ttctatagaa
     2221 aatccaaagg atgatttaac tgaagaaaat ataaaatctg tcatggattt gatagtatct
     2281 aaaaatattt tttcggttgg aggatttgac ctagcttctg ttgttgaagc caaggttgtg
     2341 gaaacaaata ccactcctta tgacttagtt atagaatagg gggctaattt tatggatgat
     2401 agtttacaac tgttaataac caatgttggt tttcctatag ctctatccat gtacttattg
     2461 gttagaattg agggtaaatt aaatacttta tcaaacagta ttgatacatt atccaaaaat
     2521 attttgatat ttaagaagta atttgacaat atatgtaagt cataaaatag gtctatttgt
     2581 aaaataatta gacatattct atgtgaaata ctaggatatt tggataattt atccaaatat
     2641 ccatagtatt tttttgaatt ttaccacctc aaatttgaat ttcactgttt tatttttgaa
     2701 tttcactatg cttaatttga aatttagaac gcatctagtt ggactcaata agatataatg
     2761 ctattctaat gacagtaata aaatgaggtg atgattaatg aaaatacatg tcgaacaagg
     2821 tattaattgt gatgatattg aaataaccat aaaatgttct gctctagata ataacattga
     2881 aaaaataatt tcattattga gtacaccgtt acttgatata aaaggtaaaa aatcaggaga
     2941 gatttataaa ctcaatgtct gtgatatttt atactttgag acagttggaa ataaaacatt
     3001 tgcttattat gaagatgatg tatttgaaat tgatttaaaa atatataaat tatcaaaatt
     3061 attagctaat acatccttct cacgaatatc taaatccatg attttaaata tggactacat
     3121 aaaaagtata aaagtaatat cacataatcg catgcaagct actttaaaaa acaacgaaaa
     3181 agttattata aatcgtcgtt atataaaaaa tattaaagaa aagctaaaca tttaataaaa
     3241 atctaaagta atttattaat atcactaatt gcaaatttat tgacctattt aataattaat
     3301 aataatataa tat
//
LOCUS       DAMKMI010000235         3227 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMKMI010000235 DAMKMI010000000
VERSION     DAMKMI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.241"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            134..781
                     /locus_tag="PC429_003662"
     CDS             134..781
                     /locus_tag="PC429_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF3849112.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(948..1517)
                     /locus_tag="PC429_003663"
     CDS             complement(948..1517)
                     /locus_tag="PC429_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF3849113.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(1522..2421)
                     /locus_tag="PC429_003664"
     CDS             complement(1522..2421)
                     /locus_tag="PC429_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF3849114.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGILLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(2432..2581)
                     /locus_tag="PC429_003665"
     CDS             complement(2432..2581)
                     /locus_tag="PC429_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849115.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(2574..3158)
                     /locus_tag="PC429_003666"
     CDS             complement(2574..3158)
                     /locus_tag="PC429_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF3849116.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         1123 a          560 c          364 g         1180 t
ORIGIN      
        1 ctatattgta actattttcg taatcttagg aaaagattac ttataaaatt aaccttatac
       61 aataatatat cgtataatct taattagcat aatagttaca atattaaaat ttaaatatgg
      121 gagtgatttt agtatgataa gacaaacacc attagagaaa cgatatgaca agctaggtcc
      181 tcatattgta acagcactca aaaaacgtta ttttgatgct tattactgta aaactagaaa
      241 tgaagctttg caacaaattt tagatttaat tcctcaaaat catgtagtat catggggagg
      301 atctgaaaca cttaaagaaa tgggcgtaca aactgctgtt agagagaaag gatttaaagt
      361 tattgataga gatttagcaa aaactccaga agaaagaact gaaattatgc gtcaagcact
      421 tctatgcgat acttttttga tgagtagcaa tgcaattagt gaagatggac aactctttaa
      481 tattgatgga aatggcaatc gtgtagctgc aatgatattt ggtccaaaaa atgttatagt
      541 tgtagctggt atgaataaaa ttgtaaaaaa cattgatgat gcaatacaaa gaactagaac
      601 aattgcatct ccagcagttg ttcagcgttt tcaagatgtc aatacaccat gctatattaa
      661 tggaagttgt atagattgca caagccctga aagcatttgt gcatacatgg ttactacaag
      721 aatcagtcgt ccagcaaaaa aaataaaggt aatattggta ggagaaaatc ttggtctata
      781 aaatatacat acatgtaaat agctctataa ataataaaat catatgaaat aacttatttc
      841 taaaagtggt agaataaaag cttctacaag actttcattc taccactttt atattctttt
      901 aatttgtgtt tctatattat taattgctcc gattttcata tatattacta actattttta
      961 ttttcataat ttttttgttc ttcatcaagc tgtttataat aatctttatt atagtcagct
     1021 agataatcta taccacctcc tgtaatcaca tttaataatt tcattgcaga ttcttgatta
     1081 actttacaaa cataattatc accagattta acaagctcta atgtaatatt aaattctttt
     1141 ttctttgcag aatcaatatc tttcatagtt ggatttggca tcatttctcc tagtttagga
     1201 ggattctttt ttaaaataga aaccatatca gtattgttaa tttttaaatc tacagtcact
     1261 ttatccttat tattctttat atctgaaatt tcccaagtca tatttttaga atacatttta
     1321 aatccttctt tatcttcgta tataagatta cctaaccctg attttgggaa atacttatca
     1381 acagtatctg catcttgtgt ttttattgaa ttaaacatac tatccacttc attttttatc
     1441 ttattatcat tagtacaacc agctaacacc agtactgata ttgcaaacat tataatacat
     1501 ataataaatt tcttcattaa attatatccc cctatacttt tttgcacaaa tattattctc
     1561 ttttttatct tttgaaaaag ttgtagtaat tgcattttgt ggacaaactt ttatacattc
     1621 accacaccta atacagtctg gactatttac atctttatat attggtatat caagcttaca
     1681 ttttgtttga catgctgtac aatttgtaca tttatcttta tcaattttaa atctatatat
     1741 tgaaatagga ttaaaaaaac catagattgc tcctaaaggg catatatact tgcaaaatgg
     1801 cctataagta atgattgaca tcaataatat caatagcaaa atacttaact tccagctaaa
     1861 taaaaatcct attgattctc ttaaattctc atttcctaaa ataagtagta ttcctcccaa
     1921 aagtgttcct gaagggcaaa tccatttaca aaaccaagga tttccacccc ctgctacatt
     1981 tacaatagca actggcaaaa taattacaaa cattattaag actatatact ttagatattt
     2041 tagatattta tcaccataaa ctttttttat ctttactgga aagggtactt tatgtagtaa
     2101 atcttgtaca agtccaaagg gacaaagcca accacaaaca aatctgccaa aaaaagcacc
     2161 aaatgccatt aaaaaaccaa caacataaaa tgagaattta tactctctgc ttgataaaac
     2221 tgcttgtaat gaacctattg gacatgctcc aagtgcacct ggacatgaat aacaattcaa
     2281 tccaggtaca cataattttt tactttctcc tccatatata gttccttcta aaaatccatt
     2341 tacatatcca tttgttaatg ctgtaaatgc aacttgagta ataagtctta tattattatt
     2401 taccttagat aataacttca tccctcttct cctaaccaat accaatacac tcaagacaaa
     2461 tcataattgc tttagttaat actgtatttg tttctccacg atatgtacct aatgcaataa
     2521 atataatact aattgcaata atagaaatgc cttttttatt atctttaata aagttactca
     2581 ttggctttta ccatttttag tctttcatct ataatttttt tccaatcttc cttactattt
     2641 gcacctacaa ccttctcacc cacaaagttt cctttcttgt ctacaaaata agttgttgga
     2701 aactccataa ctcttttaaa atcagttttt ttaagttctt cagatacagt caattgttgg
     2761 taagttgcat ctgacttttt cataatgtct ttaactaaat ttttctcttc atttgaaaga
     2821 ccagttctca tatcagtttt atcaacttct acaactaatc cctttatagc tacattggaa
     2881 ttattactct catattcttt tgataatgct tcaagctctg gcatttcacc cacacatggt
     2941 ccacaccaag tagtccagac attaataaga gtaagttctt tatttgaaaa tatctctttt
     3001 gttaccttat ttccatcaac atcctccgta tctaaattac taaaccattc actattattc
     3061 ttgttttttt ctactggctt agattctttt ttgctacatc cagaaatcat acctaaaaca
     3121 caaatacatc ctaaaactac tatgaaaaac cttttcatat ctcacactcc tagaatttat
     3181 atagtatata aaatcgctgc ttgaaataaa aataacttat cttctat
//
LOCUS       DAMKMI010000236         3141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMKMI010000236 DAMKMI010000000
VERSION     DAMKMI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.242"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            82..2439
                     /locus_tag="PC429_003667"
     CDS             82..2439
                     /locus_tag="PC429_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF3849117.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2525..2842)
                     /locus_tag="PC429_003668"
     CDS             complement(2525..2842)
                     /locus_tag="PC429_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF3849118.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1219 a          336 c          505 g         1081 t
ORIGIN      
        1 aataaaataa aaaaattgag aattttataa tatatttgta gatttgagta taaaaaagtg
       61 ctaagttgaa aggagtaaaa tatggataag gttaatttag aaaggtatag ttgtgatata
      121 aatgttggtt taaataaaaa tcaacttcaa agtagaatag atgataattt aattaatgca
      181 tttgataatg gaaaaaccaa aacatataaa caaattttta aagacaatat atttacactt
      241 tttaatctca taaatattgt tttagtctgt ttattagttt cagttggttc ttttaaaaat
      301 accttattta tatttattgc tgtaattaat actattatag gagtaataca agagataaga
      361 gctaaaagat tattggacaa tctatctgtt atagtttcaa ataaggtatc tgtcataaga
      421 gaaaaagaga aaaaagagat tgatgtacaa gaattagtat tagatgatat tatgatttta
      481 aaaacaggta atcaaatttg tgcagattca aaagttttaa atggaaaatt agaggtaaat
      541 gagtctttgg ttacaggtga atctgatatt attataaaaa ataaaggcga ttttttatat
      601 tctggaagtt ttgtagtttc tggagaagct tttgtaagag ttgaaaatat aggaaaagat
      661 aactatgcaa ataagatagc taatgatgca aagatttcta aaaaacataa ttctcaactt
      721 agaaattcat taaatgcaat attaaaaatt gtaggtataa taataattcc tttaggaatt
      781 atattgtttg caaaacaaca ttttggaaat ggagattcta tagctacatc tgtagtaggt
      841 acagtggcag ctgtaaatgg tatgatacca gagggattaa cattacttac gagcattgct
      901 ctagctgtag gaactataaa acttgctagt cataaaactt tagtgcaaga actgtactgt
      961 gtagagactc ttgctagagt tgatacacta tgtcttgata agacaggtac aattacagaa
     1021 ggtaaaatgc aagttgatga tatagtaatg ttggaagaag tggatattaa tgaaataatg
     1081 gggaatatat gtaattctct caaagatgat aatgcaacac taaatgcaat aagagataag
     1141 tataatgttt tagatacgta taaagctaag aatttaatac cattttcttc agaaagaaaa
     1201 tacagtgggg ttacctttga aaataaaggt acatatttat taggtgcatt tgagtttata
     1261 tttaaggaaa aaaataaaaa acttaggata gaagttgaaa gatattctaa aaaaggaaac
     1321 agagtaatag ttttagctca tagtgattct tatatggaag ataataatat tcctgaagat
     1381 ataaagccac tagcttttat attgatattg gataaaatta gagatgaagc taaggaaact
     1441 ttagaatttt tttacaatga aggtgtagat ataaaaatta tatctggtga caatcctata
     1501 acagtaagtg aggtagctag aaaagcaggt cttaaaacag ctagtaattt tattgatgca
     1561 acaacactta aaaatgatag tgatatatac aaagcagttg ataaatacag tgtttttgga
     1621 agggtaagcc cacaacagaa aaaacaaatg attttagcat taaaaaaaca aaatcataca
     1681 gtagctatga ctggagatgg tgtaaatgat gtactagcac ttaaagaagc ggattgtagc
     1741 attgccatgg catctggaag tgatgttact aagaatgtgt ctaatttagt cttattagat
     1801 tcaaactttg catcaatgcc aaaggttgtt atggagggaa gaaaagtaat taataacatt
     1861 caaagagcat cttctttgtt tttagtaaag acaatttttt cattcttctt gtcgttatta
     1921 acattattct tcttcagtag acagtatcca tttgttccta tacaattatc actcattggg
     1981 gctgtaacag tgggtatacc atcattcttc ttagctcttg aagcaaacaa aagtagaatt
     2041 tcaggaaatt ttttactcaa tatattaaaa aatgctttac caggagcact atgtgtgaca
     2101 gtaaatgtaa ttattgttta caatatagtt gaatatttag gttatagttc aagtgtattt
     2161 tccacaatgt gtgctatatt aactggtgta tgtggtcttt taatattgag acaacaatgt
     2221 ttgccattta ataaagaaag attattttta atagtaagta tgactattgc atttgttatt
     2281 gggattctat ttttaggtgg attattctca tttgtagcat tgagtagaga attaattctc
     2341 ataactatgg cattagcaat attaatgcca attatgataa aagttatgtt atttgtctta
     2401 gatgagatat tagagacaat tgttccagat ttaaattaat tttaaaatga tataaataag
     2461 agcagtttta gttgaactgc tctttttaga tgtgtttaat tttatgtaag aatatgtatt
     2521 tttattaatc agctatatat gagcaacagt agtcaacaac ttcattaacc tttaagtcaa
     2581 aacttgaatc actaggaaca ttaaattttt gcccttcttt ataagttaca aactcattgc
     2641 ttccaggcag ttttacatcc atacttccag caagcatctc cattatttct tcttctctag
     2701 tagaaaaagt gtattctcct ggcaacatta atcctaaagt ttttctttct ccattaggca
     2761 gtattattac tctgctactt accttaccat caaaataaac gtttgccttc tttactgcag
     2821 taacattctt aaattcactc attagataaa acctccaaaa ttatttgtac atatatatta
     2881 tataaaaata atgtaaaaaa tgctatatat ttacaaaaaa atttagtatt tattaaaaaa
     2941 attaataaga ataaaaaaga ggtgaaattg cttaaattga taaaaaatat aaaataaatt
     3001 gcaaagtaag caagaaaaga ataagttgaa gagttatata ttataaatat taaaaatttt
     3061 agtattaata aaaaagatat tacattaata tgaaaatata ggagattaga agtgtttgat
     3121 taaaaaatag ctacacacac t
//
LOCUS       DAMKMI010000237         3123 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMKMI010000237 DAMKMI010000000
VERSION     DAMKMI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.243"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     repeat_region   107..1190
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=173..201
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            1837..3108
                     /locus_tag="PC429_003669"
     CDS             1837..3108
                     /locus_tag="PC429_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF3849119.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1235 a          416 c          400 g         1072 t
ORIGIN      
        1 caatgttatc tggtaattat ttactacaaa tgctaaaaaa taattatatt ttaaaggata
       61 aaatgctgta taaattattg gggtatttta cctatacttg ttgcaaattt acataccacg
      121 tagttaatat aaaacaagat gtttttaaaa ataaacttaa tatagatatt gaatttacat
      181 accacatagt taatataaaa caacttctca tattccagat aattgttatt ttacgaaatt
      241 tacataccac atagttaata taaaacctgt agatttgcct gtttcgatat tgctcatgtg
      301 tgaatttaca taccacatag ttaatataaa actctcttgt ttcttctgtt aattttatta
      361 taccttcatt atttacatac cacatagtta atataaaacg cgttatattg ttgtataaaa
      421 ccgattaaag ttcaatttac ataccacata gttaatataa aacttgcaac atattgtttt
      481 atcgttgctg ggtctttaaa accatttaca taccacatag ttaatataaa actaagcgat
      541 attagagagc gcaaggcata gcggagcaaa tttacatacc acatagttaa tataaaactt
      601 taattttaac cacccccttt cactaataaa aaaagattta cataccacat agttaatata
      661 aaacaagacg ctatacaaga ggtaatttga aagcaatcta atttacatac cacatagtta
      721 atataaaacg ttgcatatgt atgtctaaga gcatgaaatt taattttatt tacataccac
      781 atagttaata taaaacttct attaagtgta agcaaaacgc ttggaatatg aaatttacat
      841 accacatagt taatataaaa cttatacaca aatacaactt gaagaagcgc taaagagatt
      901 tacataccac atagttaata taaaacctaa tgagtacata gataaacgta caggagaggt
      961 taatttacat accacatagt taatataaaa cgttgataat tttcgtttgt atacttttta
     1021 taatttaaaa tttacatacc acatagttaa tataaaacat aaagatttag aaaaagaaaa
     1081 agttaaaatt ttggaattta cataccacat agttaatata aaacttttcc tgttaagttg
     1141 atgtttgcaa ctttaactgc tatttacata ccacatagtt aatataaaac cccaaaataa
     1201 actaagtatt ttcagtactt acacacatac aactctctca aatttgcagt gaaccatgag
     1261 tagtgcaatt gataacattt atcacacacc ctcaatccct tacattgcaa ctgttaaacc
     1321 ctactttatc aaaaatatcg ctcactgcaa aatttatata tttttattat atcataaaat
     1381 tcaaactata aaattaaaat ggaataattt atcaatttaa gaacacctac atatttgtaa
     1441 atacttacta tattatctat tctaataatt cttcttctag ctggaactaa tataaaaatc
     1501 atctctgaaa gattaggaca tactaatata aaaataacta tgaatagata ttttcttgtt
     1561 ttaaacgata tggataaaaa agcttctgaa aatataatca aagttctctt taaataacat
     1621 tccattttat gtaagtaggc attatatttt tcttaaatgt cacttaaatg tcagttatat
     1681 ataataatag taaacaactg tatttcacta atatttgcta ttatctaata gtatttatag
     1741 tttagatttg atataataca tgtggtaaaa ttttgatttg taaaacaaaa ttttacctac
     1801 atatattaaa acaaatttaa aactaaggag acctatatgt taaaagtatt aaaaggaaat
     1861 ataattttta caaaaacttc agaaactttc acagtatttg aaaatagtta tattatactg
     1921 gaaaaaggta aagtaaaggg aatatttaaa gagataccta aggaatacaa aaacctaaaa
     1981 gtagtagatt acactgataa gcttataata cctggtatga atgacctcca tgcccatgcc
     2041 tctcaattta aaaatcttgg aatggctatg gataaagagc ttttgccatg gcttgaaact
     2101 tatacatttc cagaagaatc aaattataaa gatatagaat atgcaacaaa aatgtataaa
     2161 aaatttgtaa aagaactttg gaaatctgga actacaagaa tagcagtgtt tgcaacagta
     2221 cataaagaag cctctatgaa acttatggat atattcaaag aagttggtct tggagcatac
     2281 gttggtaaag taaatatgaa tatgaattgc cctgatactt tattagaaaa cactcaggat
     2341 tctattactg atacagaaga aattataaat aagtatagtg atacagattc tattgtaaaa
     2401 ccaataataa cacctagatt tataccaagc tgtacaagtg aacttttaaa aggtttagga
     2461 gatttatgtt taaaatataa tattcctatt caatcacact tatctgaaaa ttatgatgag
     2521 attgaatggg ttagaagtct agaacctgaa tctaaattct atggagatgc atataataga
     2581 tataatttat ttggtcaaac tcctacttta atggctcatt gtgttcattg ttctcaggat
     2641 gaaatagatt taatgagaga aaataatgtt atggcagttc actgtccagc ttctaacttt
     2701 aatgttggta gtggtgctat gcctatcaga aaattaatcg ataataatat tagattagca
     2761 cttggatcag atataagcgg tggacataca ttgtcaatat ttaaagcaat ggtatctgca
     2821 atacaattat ctaaattata ttgggttaat tctggtaaga agtataattt cttatcttta
     2881 tctgaggctt tttatattgc aaccaaaagt ggtggaagtt tctttggaaa agttggtagc
     2941 tttgaagaag gttatgattt tgatgcattg attattgatg attcaaattt aaatcatgac
     3001 aattattcta tacttgaaag acttgaaaga tttatctatg ttggtgatga tagaaatatc
     3061 attcatagat atgtatgtgg aaaactaatt gaagaaccta atatataatt ataataaaaa
     3121 ttt
//
LOCUS       DAMKMI010000238         3080 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMKMI010000238 DAMKMI010000000
VERSION     DAMKMI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.244"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            119..1561
                     /locus_tag="PC429_003670"
     CDS             119..1561
                     /locus_tag="PC429_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF3849120.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            1603..1956
                     /locus_tag="PC429_003671"
     CDS             1603..1956
                     /locus_tag="PC429_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849121.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            2147..2968
                     /locus_tag="PC429_003672"
     CDS             2147..2968
                     /locus_tag="PC429_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF3849122.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         1193 a          313 c          547 g         1027 t
ORIGIN      
        1 atttatgata taataacaaa tatataatta ttaaattata ataaatgact atagcgtaga
       61 tgtaatacta tgttatagtc atttttgaat taaacttgat atatcaaggg ggaagaatat
      121 gagaacaatt agaagagttt tagcattagg tttaacatta gctatatttc taataaatgt
      181 acctaatgta gatgcattaa catcagatac aattaaaggt aacaatatat atgagacggc
      241 tggattaata gcagataaaa agagttatga tacagcaatt atggtaaata tggataattc
      301 tatagcagat ggtctttcag caagtggtct tgcaggtgct gttgatgctc caattttgct
      361 tgcacaaaaa aataagatac caaatgaaac aaaacaaaga ttaaaaaatg ttaaaaagat
      421 atacataata ggtaaagaat tatcaataag taagtcagta gaaactgaac taaaaaacac
      481 aggggcacag gtaactagat tgggtggaga tgacagaata aagactagtt atagtgttgc
      541 taaagaggta aatggtatca aaaaagttga tgaagtgata ttaactaatg catataaagg
      601 agaagcagat acaataagtg ctgcacctgt atcagtaaga gatatagctc ctatagtact
      661 tacagatgga aagagtgtac ctttttcaac aagcaatgta aaaacatatg cagtaggtgg
      721 gagtatatca atgagcacta gtttagttaa taaaactaat gctaaaagac ttggtggttc
      781 agatagatat gatacaaata agaaagttat aaaagagttt tatccagatg catcagaatt
      841 ttatttgagt gatggatatg atttagtaaa tgcacttaca ggttctacaa ttgctaagga
      901 aaatccaatt gtattggtgt cagaaagtag tgataagtct atattagcag gagcagataa
      961 gattactaga ttaggttcaa taagtgacag tgtgtataat aagtgtgttt ctgctgcaca
     1021 aaataatggc gattcgtcta caaaaggtga gtcacctatg aaaaatgaga caagcatatt
     1081 aggtcagcca acagctagtt tagaagcgtg cttaaaatgg gcaaaatcta aaaaagctaa
     1141 tgatttattt atagagttaa taccaatatt atatgatact gctgttcagg aaggtgttaa
     1201 tcctgttttg gcagtagctc aatctgcaaa agaaactggt ttttgtaatt ttggtggagt
     1261 attagatgca tcatttaaga atccttgtgg acttaaaact tctgtaggag gttctgatac
     1321 tgataagaat gcccattcaa gatttgatac ttgggaagaa ggaatattag ctcaaattca
     1381 acatttatgc ttatatgcag gacaagatgg ttacccactt tcaaatccag tagaccctag
     1441 acatgaaaaa tctttatttg gtaaggctaa aacagttgaa agtttatcta ataattgggc
     1501 tggaggtcaa tatggacaag atttagtaag aatgatggga gaaattgagg caacaaaata
     1561 attgaagatt atcaaatatc caataaataa ggattgatta ttatgaaaaa gagtaatatt
     1621 tttagtatca tatttcctac agtcataatg gtgctaatgt ttttaacttt tgctacagat
     1681 atatttaata ttccagatat tcaatctaaa agtatttttg ttgtgggaat ggttttgatt
     1741 tttccaattt cttttttgat acaagggata atttgtgtat taaataagac taattggatt
     1801 ttatctctta ttgtgtcttt agtaacttat atagtattaa tgtatatatt tttaaatgat
     1861 tctgcatata tatatgtttt actttataca gctttttata tgattggaca tataacagct
     1921 aaactctatt acaaaattaa gacttttaaa aattaaattg aagtatataa aagtaacatt
     1981 aggagaagta tccttaatga ttttctaaaa tcagaaatag gtgcttctct tttttgtaaa
     2041 aataaaagta tgtaaataaa atttaaccat tataattttc ttttaacaaa tgtatacaat
     2101 aaacttgttg tataattaag atagataaaa taaagggagg gataatatga atttaataga
     2161 taatcatatt catactaatt tttcaagtga tggaaaggat tccatggaag atacaataaa
     2221 aaaagctata agtattggtg ttagatattt gacttttaca gaccatttag aacatgatga
     2281 agatagagga ttttgcataa attataatga ctatgttcca gtatttaata agtttaaaga
     2341 aaaatataaa aaggacatag agttattact aggtgttgaa gtgggttaca gaaaacatct
     2401 aaaaaatgaa atagaagaaa taataaattc taatcctttt gattttgtat tgtgctcaac
     2461 tcatacaatt gacaatgtac ctgttccatc aaaggagtat tttaaaggtc ttagcaaaga
     2521 agatgcgtat tataaatatt ttaatagcat acttgaaact aatcgtgaat ttggagatta
     2581 caatatatat ggtcatttgg attatatatc taggtatgga atatattcag ataatagagt
     2641 tatatacaat gactttaaag atgttattga tgaggtctta aagtcaataa tcaataatgg
     2701 gagtggtata gagctaaata cttctggtta tagatatgga ttaaatgcta tacatccaaa
     2761 tgaagatatt ctaaaaagat ataaagaact tggaggattt atagttactg taggttctga
     2821 ttctcatagg gtagaagata tatgtaaaga ttttgatgtg gcctatgata tgcttaagta
     2881 tcttgacttt aaatatgtat ctttatttaa agaaagagaa acttattttg taaatataga
     2941 aaaagttaaa tctaacaata tagcttagag tgtgttttga agtaaattaa tggatagtta
     3001 aaaagttaat agttataata ttgtatcctc tagaaaaata ctagaggttt ttattttgct
     3061 aaaataacta aatagaatta
//
LOCUS       DAMKMI010000239         3051 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMKMI010000239 DAMKMI010000000
VERSION     DAMKMI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.245"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            68..141
                     /locus_tag="PC429_003673"
     tRNA            68..141
                     /locus_tag="PC429_003673"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100..102,aa:Gly,seq:tcc)
     gene            150..266
                     /gene="rrf"
                     /locus_tag="PC429_003674"
     rRNA            150..266
                     /gene="rrf"
                     /locus_tag="PC429_003674"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            540..2558
                     /locus_tag="PC429_003675"
     CDS             540..2558
                     /locus_tag="PC429_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3849123.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1222 a          365 c          505 g          959 t
ORIGIN      
        1 gatatatatg tagtttttag agtgctaact ctaaaaaatt agttttacta atttataaaa
       61 atttaatgcg ggtgtagctc aatggtagag ttctggcctt ccaagccagc tgtgagggtt
      121 cgatcccctt cacccgctcc aaaagattat gtggttacaa tagcagagag gatacacctg
      181 ttcccattcc gaacacagaa gttaagctct ctagcgctga tggtacttgg tgggaaactg
      241 cctgggagag taggacgtag ccacgtaatc tttttttgtt gtattaaatt taataaacct
      301 acaattgtta tatgttttta tttgtctttt atagattttt ttggcaataa atgatatagt
      361 gaagataatt aaatatattc aatatattta atttttttat tttttgtttt tataattttt
      421 ttagaagttt attttttatg tacttattta taattttggg taaaaacact ttgatataat
      481 ttcaaaaatt aaaaggttaa aaaacaaagt agaaatttat gttaaataag gagattaata
      541 tgagaagaaa tacaaaatta ttaacaacag ggattctttc aatggcaatc gtcgcaccta
      601 caatggcatt tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact
      661 tagagaaaaa aagtatcgtt ttaggtagta cctcaaaagt cagtgttaag tttaaagaaa
      721 aaccagatgc agatagcatt acattaaagt ataaatgcta tgatatgcca ttggatacaa
      781 ctttaaacta caatcaatca actgagtcat atgaaggaac tatcaattat aataaagacc
      841 cagaatatct aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta
      901 aaactttaaa caaacaagaa ctagaaaaga tgggattaaa tttaaaagac tataatgtaa
      961 cacaggaatg tataattgaa gatataactt ctagaaaaga tgtaaataaa tatttaagaa
     1021 aaacttctgc acctattaca gaacttacag gaagtgatag atacgaaaca gcagttaaaa
     1081 taagtaaaga aggctggaaa aatggttcag ataaggtagt tataataaat ggggatgtaa
     1141 gtatagatgg cattatatca actccactag caactacata taatgcgcca atacttttgg
     1201 ttgaaaaaaa caatgtacct aatagtgtaa aatcagaatt aaagcgttta aatcctaggg
     1261 atgtaattat aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat
     1321 cgactgtaaa tgctagtcaa acacgtctaa agggctctaa tagatatgag acatctttat
     1381 tgatagcaaa ggaaatagat aaaaatcatg atgtggaaaa agtgtacata acaaatgcta
     1441 atggcggaga agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa
     1501 ttatattaac tgataaaaat agtattacag acaacacata taaatggtta aagagtgagg
     1561 atttacaaaa tgcttatttt ataggtggcc ctcaaatgat atcaacaaat gttataaata
     1621 aggtaaatga tataactaaa gataatgtta ctaataatag agtatatgga gcagatagac
     1681 acgaaacaaa tgcaaacgta ataaaaaaat tctatacaga tgatgagcta gaggctgttt
     1741 tagtagctaa atcagatgta cttgttgatg ctttagcagc aggtccattg gctgccaact
     1801 taaaatctcc aatacttata acaccaaaga cgtatgtatc tgcataccat aaagaaaact
     1861 tagaagctaa atcggctaat aaggtatata aaataggagg aggattgact tctaaggtaa
     1921 tgaactctat agcatcatca ttatctaaac acaatacgac tccaacagaa ccaggaaata
     1981 gtgggggtaa gacagttatg attgacccag gacatggtgg ttcggacact ggaacaacag
     2041 gtaaaccatt agggggtatt aaagaaaaag actatacatt aaatacttca cttgcgacaa
     2101 ctgaatattt acgttcaaag ggattcaatg taataatgac aagagataca gataagactt
     2161 tatctcttgg aaatagaact gctttatcta actctttgag accagattta tttacgagta
     2221 tacactataa tgcatctgat acaactggga atggtgtaga agtattttat aagcttaaag
     2281 ataaagatgg aggaactact aagactgtag ctacaaatat attaaataga atcttagaga
     2341 aatttaatct aaaaaataga ggtgcaaaaa caagaacact atcaactgac cctacaaaag
     2401 attatttata tgttttaaga aataatgata tgccagctgt acttgtagaa tgtgcatttt
     2461 tggataatga gaaagatatg agtttactta atacatcaaa taaagtaaaa gaaatgggta
     2521 cacaaatagg taaaggcata gaagattcat taaaatagtc tacaatgaga gcatagaaaa
     2581 atttaaaata aattaatcta taattttaaa tttaaactaa agctataaga taagaatgta
     2641 attataatag taataaagac tgtatataca ttgtgcatat aaatgcgaaa tgaggtatat
     2701 acagtctttt tataattaat tgtaaaatga tttatgttat atgaaaaatt tacatatatt
     2761 aaggctacag ttaattgtca agagctggag tattcatttg taatacacaa tataagctta
     2821 attactatat tttattgtgt gttttttgtg gatttttatg tcaataaatg atatagtata
     2881 gataattaaa tgtattaaat atatttaatt tttttatttt ttgagtttta taagtttatt
     2941 tttatatatt acttatatat tacttatata ttatttatat tttgggttag atacactttg
     3001 acatattttc aaaagttaaa agattaaaaa taaagtagaa atttatgtta a
//
LOCUS       DAMKMI010000240         3027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMKMI010000240 DAMKMI010000000
VERSION     DAMKMI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.246"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            376..1653
                     /gene="hflX"
                     /locus_tag="PC429_003676"
     CDS             376..1653
                     /gene="hflX"
                     /locus_tag="PC429_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF3849124.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     MSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1886..2383
                     /locus_tag="PC429_003677"
     CDS             1886..2383
                     /locus_tag="PC429_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF3849125.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2433..2957
                     /locus_tag="PC429_003678"
     CDS             2433..2957
                     /locus_tag="PC429_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF3849126.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1257 a          266 c          530 g          974 t
ORIGIN      
        1 atgcaaataa ctacttaaat ttaagaatag ggttaaatat aaaatgataa ttacatataa
       61 gttaattaaa ctaatgtatt tttaatatat atttagaatt ttaatccttc ttatgatata
      121 agtagcttgt tttgtattac ttagatttgt aaggagtttt tttattgaaa aaactgcata
      181 atatatttgg gaggaggtgt aagaaagtga ttttttatat aagattgaga aaataatgca
      241 cgattatggt tattataata gtaaataaga agtgtcattt aaggataaga taaaaagcaa
      301 aattcttgat gaatttaatt atagagccat aatctcgtgc tatagattaa ataaaaaata
      361 gcagataagg agaatatgga tatggcgaaa aaaggaataa cagtaggatt aaacataaat
      421 aataaatctg aggactttaa tgaactaatg attgaacttg aaaacttatg ttcagcatgt
      481 gatattgatg ttgtagggag tataactcaa aatgcaaagc aggttaatag agcattttac
      541 attgggacag gtaaagttga ggaaatatta aacttaataa agaaagaaaa tatagagata
      601 gttatatttt acaatgaact ttctacatca caactaaaaa atcttgaaga aaagcttaat
      661 tgtgaaatta tagatagaac ggcattgata cttgatatat ttgcacaaag ggcaaaaact
      721 agagaagcta aacttcaagt agaagtagca agtttaaaat atatgttacc aagacttata
      781 ggttcaaatg aaaatttagg acgtcaaagt ggtggagttg gtactaaaaa tagaggttct
      841 ggtgagaaaa aattagaact tgaccgtaga agaatagaag aaaagataac atctttaaat
      901 agggagttgg atgatttaaa attccaaaga gaaactcaaa gaagtatgag aagaaaatca
      961 aatcttccaa atgtagctct agtcgggtat acaaatgctg gtaaatcttc aataatgaat
     1021 aaactagtag atatatttaa aaactctgaa gaaaagaaag tgtttgagaa aaacatgtta
     1081 tttgctacac ttgaaacttc tgtcagaaat atagttcttg caaataacaa agaattttta
     1141 ttatctgata ctgttggttt tgtaagtaat ctaccacatg acttagtaaa agcatttagg
     1201 tcaacattag aagaagcatg tgaggcagac gtactgttac atgttattga tatttcaaat
     1261 ccaagctata aaagtcatat taaagtgaca gaagatactt taaaacaaat aggtgcagat
     1321 ggtataccta tgatacatgt gtataataaa attgatttaa tagatgtaga cgtattggat
     1381 agaattttag atagtattga taaagaaggt atatttgttt cagttaaaaa agatataaat
     1441 atagataaaa tgataaaatg tatttgtgat agcatattta aagattatgt aagatgtaag
     1501 ttcttaattc catatgataa aggtcatgta gtttcttatt ttaacgaaaa tatgagcatt
     1561 ataaatacag aatacagaga agatggagct attttagatg tagaatgtag caatatagaa
     1621 tataataagt ataaaaagta tgctttagag taaattttga aaaacttatt atagcctaaa
     1681 atatatttaa tttataattt attataaatt gattgtaaat atgattttat gaaacggtat
     1741 agccataatg ctatgccgtt ttgccattta taataaaaat aaatttattg acataatagt
     1801 aactaattac tataatataa agtaattagt tactatttaa aattatcaaa aaattaaaat
     1861 ctaagataaa gagaagtgta taataatgga tgcagaaact ttaatgaaaa aatatatggt
     1921 agatgatgat agtagaaaac aagctatatt tgcaagtata tttgtaatgc aaaataaact
     1981 tcaaactaca tgtgataaat tagacccaga attaacaatg aaacaatggt tacttttagc
     2041 tattgcggac tctgtagatg aaactcttac attaaccaaa cttggagaat taatggggtg
     2101 ctcaagacaa aatgtaaaaa aactagctat tgctttagaa aagaaaggtt ttatacaaat
     2161 tgagcagaat gaaaaagatt caagagctat ctgtcttgtg acaaaagata gagtgcaaga
     2221 gtattctaaa aaagtaagag gtatgcagga aaaagtatta cagcttttat ttgaagattt
     2281 tacagagaaa gaagtggaac agttattttt tggaattatg aaattaagta taggaattga
     2341 aaatgtgaaa aattatgtag aaaataatga cgttggagaa taagcaagta tcaaatcaac
     2401 tttgtatgac atcagagagg gggataattt atatgaaaaa tgtagtagtg atttataaga
     2461 gtaagtatgg ttcaacaaaa aaatatgcta aatggctttc agaattattg tcttgtgata
     2521 tttttgaaac taagaatatt aatatagaaa aggttatgaa gtatgatacg attattttag
     2581 gaggaggtct ttatgcttgt aatatagcgg gaatttcttt cttaaaaaaa aattataaag
     2641 aattgcagaa aaaaaattta gtagtatttg gcgtaggagc tgctccatat gatgatgaag
     2701 taataaagaa attaagagaa gtaaatttta aggatgaatt aaacaaaatt ccttgctttt
     2761 attgtcgtgg ggcatgggat gagacaaaaa tgtcttttat agattatact ctttgtagtt
     2821 tttttaaaaa atctatagct aaaaaagaac gtcaaggtct taaaccagca gaagcacctt
     2881 tattgtctgg agaagcattt gattggtgta ataaagaaaa tttaactcca atacttgatt
     2941 atataagaga actttaagag gtatatattt ataattattt attatctaaa gttagatttc
     3001 atagaaactt actagacatt atttata
//
LOCUS       DAMKMI010000241         3002 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMKMI010000241 DAMKMI010000000
VERSION     DAMKMI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.247"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            133..501
                     /locus_tag="PC429_003679"
     CDS             133..501
                     /locus_tag="PC429_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF3849127.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            504..875
                     /locus_tag="PC429_003680"
     CDS             504..875
                     /locus_tag="PC429_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF3849128.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1020..1475)
                     /locus_tag="PC429_003681"
     CDS             complement(1020..1475)
                     /locus_tag="PC429_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849129.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1514..2779)
                     /locus_tag="PC429_003682"
     CDS             complement(1514..2779)
                     /locus_tag="PC429_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF3849130.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1122 a          418 c          408 g         1054 t
ORIGIN      
        1 aaactcaaag ttatattagt aaaaattgga attatttata atatactcca aaatttaaaa
       61 ataatggttg aaaaatctaa ttaagagtac tataatttaa ttaagtaatt acttaatcaa
      121 aacgaggtac ttatggaaaa cttggttaaa atatttaaag cattgagtga tgaaactaga
      181 cttaacatac ttatattagt atccaagaga aatatatgtc aaaaggggat ttctaaatat
      241 ctagggattt cagattctgc tgtttcacaa catataaaaa tattaaaaga tgttgggata
      301 ataacaggat ataaagaagg atactatgtt ttatatcata ttaataaaga atcatttaat
      361 atatgtgtag attttataaa ttctatgtta agtaattcta gtgaaagctt tatagatgta
      421 tttgatgtaa atacaataca tctaggatgc agtaaagaat gtaaatctat aaaaaaatgt
      481 tgtaagagaa gggagaaata ataatgaaag tatgtatacc agtagaggag aataaaggat
      541 tggatagtaa accatatgga cattttggtt ctgctccaat ttttgttgtg tgtgatttag
      601 aaagtggaga agttaaatct ttagataatg gagatttaga tcatgaacat ggaaaatgtc
      661 aaccattaaa agcattgtca gggacagctg tagatgcagt agttgttgga ggaataggac
      721 aaggagctat aattaaatta aatggaatgg gaataaaagt ttacagagca gaaggtgata
      781 ctataagtgc taatttagac ttacttaaaa atggaaaatt agtagaattt ccatcagacc
      841 atacttgctc acatgacgga tgtggacatc attaaaaaaa aacagctgat gaaatatata
      901 cttcatcagc tattttttct tttaataaat acgattttat tcttttaaaa ccaaaattta
      961 gatttttata ttttttgtag tatccttaat aatatttcaa gtttacaagt ttactacttt
     1021 tagatttttt ttgtatgttt ttctatatag tcaatatcat ttaatatgtc attgagttct
     1081 ttattaagtg tttttaaatc atcaccatct acattgtagt ttaaaaatag ttcctctaca
     1141 ttatcaatga tagaattgac ttttctagta acttcaacaa atgtcttaaa gttactacta
     1201 tttttagcat ttagaaattt ttttaaatcc tttgatataa atgatactat ttcatattca
     1261 tcattaaata gacctctacc aataggatat ggttcaattc tctgtaaaaa atattggtct
     1321 ttagcattaa tattataagt ataagatatt tcccaaccgt ctcctaaatc aaacttgata
     1381 aaatataatg tactacttac ttttgatatt cttgtggcac caagctgtct taaaagaact
     1441 tcaatcgaat caccttgaac agtattaata ttcatatata cacatcccct ttagtattat
     1501 tatactatat tttttaaaat tttataatta aataaacagt agatataata atagatataa
     1561 tcataagtgg aaaacctatt ttgaaatact ccttaaaaga gataggatga ccgtgtttat
     1621 tacctatacc tgctagtaca acattagcag atgctcctat tagtgttcca tttcctccaa
     1681 gacaagctcc aagagatgtt gcccaccata gtggcattac atcaatacct tcagcttgca
     1741 tagtcaaaat aagaggaatt agtgttgcaa caaatggaat attatctaag aaagaagaga
     1801 ctatagctga caaccataat atcaacaaca ttgtaaatac aagatgtcct tctgtaagtc
     1861 caatcaatgc ttgagccaat ttgtttatta tcccaacttc tactaatcca ccaactacta
     1921 caaataatcc cataaagaat aaaattgtag accattcaat acctgccata atttcatcta
     1981 catcttgttt tcctattaaa agcattatag atgctcctgt cagtgctaca acagaagaat
     2041 ctatgtggat tgtactgtga aacataaaac caaaaagtat gaatgctatt aatataaggc
     2101 tttttataag tagaggttta tctttaacag attttttttc atctagtttt aatattgcat
     2161 ttttagctct ttcattcaca acaagttctt ttccataaat aaatctaaag catattatag
     2221 ttatagcaag tattactata acagcaggac ctaaatttat tacaaaatcc ataaaactaa
     2281 gattggcagc actaccaatc ataatatttg gagggtctcc tataagagta gctgttcctc
     2341 ctatatttga agctaaaatc tgtgttatta aaaatggaac tggattaagt cctaatattt
     2401 gtgtaataac tatagtcatt ggtccaataa gtaagacggt tgtaacatta tctaatacag
     2461 ctgataaaat agctgttata atagcaaagc aaatcattat cttccatggg tcacccttag
     2521 ctttctttgc agtccagata gctatatatt caaataaccc cgagtttttg acaacagcta
     2581 caaatagcat cataccaact aatacaccaa tagtattgaa atctatatgg cttaatcctt
     2641 catcaagtgt aagtatattg aatattatca ttaatagtgc accaccaaca gctgctacgg
     2701 atctgtttac cttttctgta ataatagctg ccataactat taaaaatata catatagcta
     2761 ttatttgttg aaattccata atataagtac ctccatttgt attattttgt gcctaatgct
     2821 agtactatat atagtattac ttttttattg ttttttcaat ggatgtatga aagtatatta
     2881 tttaacaaag tttttgaaaa attgacagaa tttaaagaaa atttttgtta tttaaataaa
     2941 ttttaaaatt attccattga taataatatt aaacgacaaa cgaaaaaata taccctttaa
     3001 ac
//
LOCUS       DAMKMI010000242         2969 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMKMI010000242 DAMKMI010000000
VERSION     DAMKMI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.248"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            135..1028
                     /locus_tag="PC429_003683"
     CDS             135..1028
                     /locus_tag="PC429_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF3849131.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            1465..1956
                     /locus_tag="PC429_003684"
     CDS             1465..1956
                     /locus_tag="PC429_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849132.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            1984..2931
                     /locus_tag="PC429_003685"
     CDS             1984..2931
                     /locus_tag="PC429_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849133.1"
                     /translation="MRITKTTFNDVFSSDANESIIKVEKYVDKKCASVFEILTYTIIV
                     TNTNRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         1301 a          263 c          417 g          988 t
ORIGIN      
        1 attataacaa tagtagtgct tttaattaaa tatatttcat aaatcttcat aaaatcttca
       61 aaatttcgta aagaatatgt cacaaaagta aggtatatta tattcagcag atagtataag
      121 ggaggacaat atttatgaag aataaaaaat tcattgttat aatactgata atatcaattt
      181 taataggtgt attagtgaag gaatattcat ctagagagat aaaaaaggat gatataaatg
      241 tcagtaagta tataaaatat gctgatttag caagtaaaaa taatgcacaa gttaactgga
      301 agtatgtagc ttccattgtt gctgtgttaa ataaaaataa cttaaaaaac gttaaggatt
      361 cacagataca agaagtatca gatttatttg ttaagaattt ttctaaaaat aataaaataa
      421 ataagttaag tgatattttg gatgagttag aatttagtaa tagacaaaaa agattggttg
      481 ataattatat agacaatctt aaggattatg gtataaaacc agaaagattg aagtctgata
      541 caaaatatat gaagtttatt gatgaaatta aaacagaagc aatacaaaat tataaggatt
      601 ataaaatact tccatctata actattgcac aagctataat agaatctagc tggggcaagt
      661 caactttggc aaaacaatat agcaatctat ttggtataaa ggctgacgct tattggaaag
      721 gtaagtcagt gactttagaa acgaaagaac atttagatac aattatagat gataaattta
      781 gaatatatga tgataaaaat gaatctataa aagaccatgc aaaattttta gccacaaata
      841 aaaggtataa aaacaatggt gtatttgatg caaaaacgta tatatatcaa gctaaagcat
      901 tagaaaaggc aggttatagt actgcgaagg atgaaaatgg caatagtata tatgcagctc
      961 gtctaataga attaattcaa cagtataatc ttcaattaat agacagtgaa attcaaagtg
     1021 aaatgtaact ttgattaaat ttttattgat aaggctatga tttttaaaat catagcctta
     1081 ttttgacatg cttttacttg taattattaa atcgatatca ttatctttca aaatttactt
     1141 tatactgtaa atcaaattat ttaagttaaa acttggaatt atttataaaa ggattattta
     1201 ctttatctaa attctgcact taaagaacat cttttccctt aagactgacc gttagtctat
     1261 aaaaattata attacttata aaaaagtgaa tatatatata aagttttcag aaagactttt
     1321 acttgtaaat tgaaatcaat gaagtaaaaa aggattcttt cttaaaagta aataacaact
     1381 agatagtgaa taataaaaaa tggtaatttg aaataatttt tatgaatagt attaatatat
     1441 agatacaggg agattgatgc gattatgtat gaaaataata aggtaatata caaccttgaa
     1501 agatgtgaaa aactagaaaa gactctttat ttatctcaat ttaatacata taaaaatttt
     1561 aaattagctt ctaaaaaaac tgtaataaat tcattagaga catatgttta caatatgaat
     1621 attaacaata taagtcttgt tgatactata gaatgtatat cattagaaga tgaacaactt
     1681 acaggaaaga agttgatttt agttggtgat atagatattg atgcaatatt agattatgca
     1741 ggaaataaaa gaaatagaaa ttctagtaaa aaaaatacat ttaaattaaa aatacccttt
     1801 agtacattta tacaaatgcc tagaaagata gaaaataaag ataaaattaa tttaaagtat
     1861 cttatacaag atattacttc atctatttta gatgataatt tatttattag tgttactgca
     1921 attataagtt atgaaaatag ttccaagata gagtaatctt taaattatag aaaggtggat
     1981 gcaatgagaa taacgaaaac tacttttaat gatgtatttt caagtgatgc aaatgaaagt
     2041 attataaaag tagaaaaata tgttgataaa aaatgtgcat ctgtatttga gattttgacc
     2101 tacactatta ttgttacaaa tacaaatagg tacaaaactg gaaatatatt tttcaaagat
     2161 tatatttcta aatatataga gtttataaat aatactgtaa aagttaatgg cattataaaa
     2221 cgtggattag acccacagca aggtttttac atagggagaa tagatgcaag ttgtaaaaaa
     2281 ataatatctt ttaagagtgt agttttacca aattctgcac ataggattat aaagaatagt
     2341 gctaatatat actattatta taagtgtaat ctagataaat tcccaacaag aatatcaata
     2401 gaaagtaata gagtttatac taatgtaaat aaaattgtat tcaaacaatt gaatataagt
     2461 aatactttaa aagctcctaa aaatctaaga gatatattaa aagtaaatgt aaatagtaaa
     2521 atatttgatg ttaaacctat gaaaaatctt ataaacaaag gtagtaattt gaaattatgt
     2581 aacttgatag tttttgggtg tatagaagtt gaaatagact atagttgtaa aaataggagt
     2641 aattttaaaa cagtaaataa gtctaatgac attaaaaaag ataagtatta taaaaatgat
     2701 atacaaaata acaatgaaaa tataaacgat tgtaaaaagc aaataagttt tggtgataat
     2761 aaggtaaaaa aaataacaaa aacttttgga ttttcttgtt tcatttgttc tccagttgga
     2821 attatttatg aagatatgaa aaatataaat ataaaaattg aacatacatc aataaatgaa
     2881 ttaaatccag aagaattatt tgtaaataca tcacttttat tgtattatta agtattttaa
     2941 agctaaaact taaacttatt aaaatagca
//
LOCUS       DAMKMI010000243         2922 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMKMI010000243 DAMKMI010000000
VERSION     DAMKMI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.249"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            100..1209
                     /locus_tag="PC429_003686"
     CDS             100..1209
                     /locus_tag="PC429_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF3849134.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1586..2770)
                     /locus_tag="PC429_003687"
     CDS             complement(1586..2770)
                     /locus_tag="PC429_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849135.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1121 a          441 c          403 g          957 t
ORIGIN      
        1 tataaataat aattaaaaaa tttactaaaa atacttataa atatgatatt attaattggt
       61 taataaaaaa gaaaattttt catgaaaggt aagataacaa tggatttcaa aagcttaggg
      121 attagtgaaa atacaataaa tattctaaaa aaatcaggaa taacaacacc tacacctata
      181 caaaaagaaa gtattaaact aataaaagaa ggaaaagatg taatagcaga agctcaaact
      241 ggaacaggaa aaacactagc ttttttactt ccgatatttg aaaatatatc attagatata
      301 aatgatatac aagtacttat actttctcct acaagagaac tagctattca aataacagaa
      361 gaagctatga aacttaaaga atctaaagat gtaagtattc ttgcagcata tggaggaaaa
      421 gatattggtt ctcaaataaa gaaactaaaa ggaaatattc atatgattat agctacacca
      481 ggtaggcttt tagaccatct aaatagaaaa acaatagacc ttagtaagtt aaagactttt
      541 gttttagatg aagcagacca aatgttgctt atgggattta aaaatgaagt ggaagctatt
      601 ttaaaagaga cttcaaacaa aaagcaaaca ctatgctttt ctgcaactat aaattctcaa
      661 gtaaaaaaat tagcttatag atacacaaaa aatcctgttg tagtttctat tcaaaaagaa
      721 gaaatcactc ttaataatat aaaacaagaa gtagtagaaa ctacagatag aaaaaaatta
      781 gatgccttat gtaaggtact agatgaagat aatcctttta tggcaataat attttgtaga
      841 actaagagaa gggttgacaa tcttgaagaa gctcttgcta taagaggata taattgtcaa
      901 aaacttcata gtgatattgc tcaatcaaaa cgagaaagaa taatgaaatc atttagaaat
      961 ttagatatac aatacttaat agctacagat gtagcttctc gtggtcttga tatatctgga
     1021 gtaagtcata tatataatta cgatttacca gaaacaccag aagactacat ccaccgtatt
     1081 ggtagaactg gtagagctgg tgaagaaggc tatacatgtg cttttattga ccctaaaaat
     1141 gaacgaatgt taagtgaaat agagacggct atagaatcta aaattagtag aagaattata
     1201 gaattataat ttatagattg cttaaaatac caatgccaca caaacactgt aatatataga
     1261 aagctacaga aaacatgtat caatctatac tttgctgtta tactatttta tattaaagaa
     1321 aaaagaggct atccaaagta ggattagtct cttttattgt caaaataatg aaaatatatt
     1381 tgacttcata taaaaaagag tgccttataa actaaaagtc cattttgaga caccctcttt
     1441 ttatctttta ttcttagttt tatattgctt gtttatagtt gtttaatatt agattttgca
     1501 aataatctta ataaacatgc agatatatta tctatagtat tcttatctat agtattctct
     1561 ttagcaatat tttttgctac tacatctaat ttacattttc atttaattcc gctggccatt
     1621 tagcttctgg attttccttt gcaaacttct tattcttata atgtcctagt gtaaggaatg
     1681 gaattactat agtgaataga ccaagataac cacaatatcc atatccatat ttaacaatat
     1741 tacttaatcc tgtaagtgat ataagcattg acactaccat tattgcacaa gctgtaaata
     1801 cccttctttt cattcttgaa gaaaggaaag aaagtgcttt atggtcttca aatctattta
     1861 caagaccaaa tatagtagtt actcctgtag acattaaaca aaggaataat gcaacattat
     1921 aaattatgta tatagtatga tttccagatt gacctgcaac ataaagagta gggagtgttg
     1981 tttgcccagc agcaataatt tctctatacc atcccattag catagttaca gcaagtccta
     2041 atgcaattgc attcataaca aatgaaatta tcattgcctt agtagcttct tttgagttct
     2101 taagaagtgg tcttgaacat cctgcaagtg taggtataac aaccgattga aaacctgcat
     2161 aaataaaagt attaagtatt ggcttgatat agccaggtgt gaaactcgtt ctatttgata
     2221 atatttcagt tatttctggt actttagctt ttataccaac aataaaaata gtaagagtac
     2281 atactagaat agctattgtc attgctgtag ctgctttaga tacaagcata gcaccaaata
     2341 tagtaaagac aagaagtaca actccaataa taaatacagc tacaaaataa tttactccta
     2401 tactttgaaa tactgctgct gcacctgcta taacagcact tacagctatt aaaaccatta
     2461 gatagtaata tatctcccat attatttcaa gttttggata tggtctccaa agctcacaaa
     2521 ataaatcctt ataattccta caattgttat tattatacat tatgataact tctctcatgg
     2581 tagcagtaag tataatcatt gctaaaattg ccataagtgg tgctgtccat ccatactgca
     2641 catagtactg agtttcttga ttccctgtag caaatcctcc tcctgcatgt gaactaaaaa
     2701 gaacgcttgc aacaccaaaa atggctaaca tagagcccgt tacttttgct ttctttacac
     2761 ttttttgcat gaaatcatcc tccctaaaat gatattaaaa gtttttattt taacacttta
     2821 ttgataaaac aataacattt ttgctcatta aattcaacta tatttttcca atgactttac
     2881 attttcaact tatttctacc tttatattaa atatttttta ta
//
LOCUS       DAMKMI010000244         2855 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMKMI010000244 DAMKMI010000000
VERSION     DAMKMI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.250"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            329..2140
                     /locus_tag="PC429_003688"
     CDS             329..2140
                     /locus_tag="PC429_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF3849136.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2228..2809)
                     /locus_tag="PC429_003689"
     CDS             complement(2228..2809)
                     /locus_tag="PC429_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849137.1"
                     /translation="MLYLLKINQFEKSYDIGIFKNEQSIYKFIAKIPFVKKDNSNFNY
                     TNYYMEFEDIPEYYEVNFNNYLYIITRFSFIPDGTEIFFTWEEIHCWDEKVATEKTFI
                     NGETLVDSYNFPNNEVKDYIQKRESLYIETKKYYEEKGLKIERHALGSEDGEYVELTN
                     DSILYLLDIEAVEIREQSKNIEEFLYKYKKRFK"
BASE COUNT         1074 a          302 c          399 g         1080 t
ORIGIN      
        1 ttatgtatat gccaaattta aaaagagaca taaagacatt atacaaatat attattgggg
       61 tgatatttta taagaaaaga agtgatgcag taaattaatt gagcaattta aaaatgattt
      121 aatgatagtg ttgatgaatg tttaaaaaga aattttagaa aaacgtatat tgctgtttgt
      181 gttggaggtg atgaatttgt ataatttata ggatataaat gattaaacta tttaagataa
      241 atacatatca ctgtaaaata ttgataatca ttttttataa gagattgatt atttgcaaat
      301 tatatatatt acaaatgagg taacaataat gaagaaaaaa tatgattttt taatttattc
      361 tgctatttgg ctagttatta ttattctttt tatattttct ctatatcaca gtatagagca
      421 tattaaaatt ataagtcaca ctggtactat tcgcagtgaa actcaaaagg ttgttaagca
      481 agaattgaat aatgaaagaa atgatgactt gataaaacga ctggataata ttctgataaa
      541 gctaagaaca ggtaatggag aaaatggatt tcaaagatgt gataataaag aatttcaaca
      601 aaaactaaat caaatggatt ctatgtggga atctatgaag aaagaaatta taaaagttag
      661 aaatggtgct tcaggtgata aattatataa attgagtgaa gagtattctg tgttgtctaa
      721 tcaaattgtt tttatatctg aaaaacactc taatgcaaaa ttgtattctt ttgctactgc
      781 actatttatt tatcttatat tctcaacaat aagtctttta atttgggaat attataataa
      841 aaaaaggttt aaaagaatat tttatactga taatttaaca aaaataaaaa atcaggttgc
      901 atttgaaaac agagcgattg aaattttgca caatgcatct aacaaagagt atgtactgct
      961 aaatattgat attgacaatt ttaaatatat taatgataca catggatatg aatatggaga
     1021 taaagttcta ataatagttg cagctgcact ttctaaaact tttaatatca aagaaacttg
     1081 tgcaagaatt ggttcagata attttgtaat acttgcaaaa tatagagatt ctcttttaga
     1141 ggatattcgg gaaatgctaa ctaatgctat aatctctgaa ttagatatga atgtaactca
     1201 aactatatct tattgtatag gagcgtactt agttgaaatt gataatttag gttataaatc
     1261 catcaattct ataatggata aggcaaatat tgcacacaag gtaagtaaaa caagaggaat
     1321 ttcctccaca gtatggtata atgaaaatct gttaaaacag ttacaaatgg aaaatagtat
     1381 ttataattat atgtataaag cgatagaaaa tgaagaattt catatgtatt tgcaacctaa
     1441 atttcaaata tcttctttaa atgttgtaag cgcagaagct cttgtaagat ggttttctcc
     1501 agaacttggt tttttatcac cagatgaatt tattccattg tttgaaaaaa gtggatttat
     1561 tattgaattg gacttttata tgttaaaaaa agcgtgttca tttgtaagaa aaacgtttat
     1621 gaaaaaaaat caatatacat atccgattgc agttaatttt tcaagggtta ctatttacca
     1681 aaatagtttt tatcaaaggt ttttagatac tgttaaagaa tatgaaattc cttttaagta
     1741 tatagagata gaggtaactg aaagtgcttt taatgaaata tctcaaccag ttatttcaat
     1801 tttagaggaa cttaaaaagt taggattttt aatttcaatg gatgattttg gttcaggata
     1861 ctcttcatta agtcttttat gttctctatc tattaatgga ttgaagttag ataaaagctt
     1921 acttaaagaa acctttaacc gggagaaagt atatagtatt attcaatgta ttattgaaat
     1981 gtcacatcgt ataggcatgt ctgttgtatg tgaaggtatt gaaactaaaa aagacttgga
     2041 atttttaaat acagttaaat gtgatgtagg acaagggttt tatttttcta aaccgataga
     2101 agaaaaagaa ttttttaaca aatatgtaac taaaaaataa tataattatt tttatcagtc
     2161 tatacattga gtcattttat atctcgttaa atgactcaat gtactgttat gtattttaaa
     2221 atataaatta tttaaaacgt tttttatact tatatagaaa ttcttctata ttttttgatt
     2281 gttctcttat ttcaactgct tctatatcta gtaaataaag tatagaatca tttgtaagtt
     2341 caacatattc accatcttct gaaccgagtg catgtctctc aatttttaat cctttttctt
     2401 cataatattt ttttgtttct atatataatg attctcgctt ttgtatgtag tctttcactt
     2461 cattatttgg aaagttatat gaatccacta atgtttcgcc atttataaaa gttttctcgg
     2521 tagcaacttt ttcatcccaa caatggattt cttcccaagt aaaaaatatc tcagttccat
     2581 ctggaatgaa tgaaaacctg gtaataatat ataaataatt attgaaattt acttcataat
     2641 attcaggaat atcttcaaat tccatatagt aatttgtata attaaaatta ctattatctt
     2701 tttttacaaa gggtattttt gcaataaatt tatatattga ttgttcattt ttaaaaattc
     2761 ctatatcata acttttttca aattgattaa tttttaataa atacaacatt ctttctcctt
     2821 ttattcaaaa tataattttt ataaatttac taatc
//
LOCUS       DAMKMI010000245         2843 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMKMI010000245 DAMKMI010000000
VERSION     DAMKMI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.251"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(397..1104)
                     /locus_tag="PC429_003690"
     CDS             complement(397..1104)
                     /locus_tag="PC429_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF3849138.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(1979..2764)
                     /locus_tag="PC429_003691"
     CDS             complement(1979..2764)
                     /locus_tag="PC429_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF3849139.1"
                     /translation="MNFENDFITPEFRKKYIGLVLDNAQNINKVYTTTFPDKEIIKQV
                     IDKDEKDILIFSHHAMGYIASDEGFPFHDIPLSYMEEMKNRRISFYVLHSPLDNYSDY
                     STSVSFAKAMGLEIVKPFCKYDDKIEVGVICKTSLKSIEEIKELIKKSVGHDVKLYDY
                     GDSVLKDGLVAIAAGGGSYPFVAREVAELGINLYITGFTKPLKHFEPVLEFHQIAKDN
                     LINVIGATHYSTEKFACMGMVDYFKNLGLESEFLQGKYYLEDL"
BASE COUNT          985 a          459 c          252 g         1147 t
ORIGIN      
        1 gtatatatac ttaatcattt tgaaatttga tttaaagtat aataaaatat ataaaatggt
       61 actataaaat ttttaatctt catcaatacc ttttttgctt tcattgttaa aactattttt
      121 tctattagtt tatttcttat aaaacaaaca tttagtatta aaatatatat cacacaatag
      181 tatttgcaat ttaactttaa aaaataaaaa tacccaactg acctaagcaa ctcgattggg
      241 tataaataat ctaaaaaatc atttttgtat aaactcttta tttaacttac atttttattt
      301 accttctact ttaattcaac ttatatttct atgcttctta tactctcatt caacttatat
      361 ttctacttat cttatacttt tataaattca tcacaattat ttagttctcg ctatcaaaga
      421 aattgtatta aaatactttc cattctctac ttccatcatt tttttgtcat ttatagcata
      481 tgaattatca caatttatcc aatccttcca agcttcatca tgacaattta aagcttcaca
      541 tttctctaca atcacagact ccgattcaga ccaaagttta ttccaccatg taattgaatg
      601 gaagttcata ttatcttgcc aaaatggaat gagttcatct ggtatacaat ccacgaaatc
      661 ttcttttaat ccaggcattg ccatagcaag tattcccccc tcttttacaa gaggtgatat
      721 atgattgtct aaaaatcctt ctttgtttcc aaaatagtga taagaatcga tacttatcac
      781 cgcatcaaag aaaccttctg cataaggtag ttcatgtgcc tctgcctgta ttggaaatat
      841 tttatcatcc aatttaaact ctttaaatct tttatagttt tcagttggtt ctatccataa
      901 atctgttgca aatacagtag catcaaattc ctttgctaaa aatatcgaag atataccttt
      961 accacaacct aagtctaaaa tacgcattcc cttttctagt tttatcttac ttgttaattc
     1021 ttccaatatt tttatacaat taggtcccat catatttttc atgagaaagt ccttatcgta
     1081 tttattactt tttgtaaata tcattctatc tttcctttcc ctatcagtta ttatttatta
     1141 aattttatac gtttgatttt ttatttttta tatgcttctt atattgagca atctcttttt
     1201 tattcattct ttaacagaca attttaatgt gtaattttat ccactctgtg atagtaactt
     1261 taatgaataa tcttatctac tgtgatagtt attttagtga gtaattttat ctactccaca
     1321 gcaatcgctt taatgagtaa tcttatccac tctatgataa ttattttagt gagtaatttt
     1381 atctactcta cagtaatcgc tttaatgagt aatcttatcc actctatgat agttatttta
     1441 gtgagtaatt ttatctactc cacagtaatc gctttaatga gtaatcttat ccactctata
     1501 atagtcactt taatgagtaa tcataatttt tatatcattt tagatactta ctctaataag
     1561 aagtcatttt ctaatactta ataagtttta attacttcca ctttaaccta tatactaatt
     1621 aacttattta atttagtgca tatttgacac tttacctttt attataccat ttattatcat
     1681 aaatataata attctatttt tttaaaataa atatttataa taatctaaaa taaatattta
     1741 taaattctaa tcaatcctaa ataataatct aaaatacatc aaaaaataaa ctaaaaaata
     1801 tcgaaaaata atttaaaaga catcaaaact aaatcttcta atcagcaatt aaaagtctat
     1861 tttcttagtt taaaaaataa agatagattt tttcaaaaca ctggataaat aggtaaaaaa
     1921 ataaacagtt agtaaaataa atttttacta actgtttatt tttattgttt aatatgtcct
     1981 ataaatcctc taagtaatac tttccttgca aaaattctga ttctaaacct agatttttaa
     2041 aataatcgac catgcccata cacgcaaatt tttctgtaga ataatgagta gcacctataa
     2101 cattaattaa attatctttt gctatttgat ggaactctaa tactggttca aaatgtttta
     2161 aaggcttagt aaacccagta atatatagat ttataccaag ttcagcaact tctcttgcta
     2221 caaatggata acttcctccc cctgctgcaa tagcaactaa accatctttt aatacagaat
     2281 ctccataatc atacaatttc acatcatgac caacagattt tttaattaat tccttaatct
     2341 cttctattga ttttagtgat gttttacata taactcctac ttcaatttta tcgtcatatt
     2401 tacaaaatgg tttgactatt tctaaaccca tagcttttgc aaaactaaca gaagttgaat
     2461 agtcactata gttgtctagt ggagaatgta atacataaaa agaaattcta cgatttttca
     2521 tctcttccat ataagatagt ggtatgtcgt gaaatggaaa accttcatct gatgctatgt
     2581 atcccattgc atgatgtgaa aaaattaaaa tatctttttc atctttatct ataacttgct
     2641 taattatttc tttatcagga aaagtagttg tgtatacttt attaatattt tgagcattat
     2701 ctaatactaa tcctatgtat ttttttctaa attctggagt aataaaatca ttttcaaaat
     2761 tcataaaact ccaatcatca ttaatatttt taatattaaa ttctaaatct aattttttat
     2821 aaagttcaat tgccttaata ata
//
LOCUS       DAMKMI010000246         2830 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMKMI010000246 DAMKMI010000000
VERSION     DAMKMI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.252"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            183..1106
                     /locus_tag="PC429_003692"
     CDS             183..1106
                     /locus_tag="PC429_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF3849140.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1081..1908
                     /gene="panB"
                     /locus_tag="PC429_003693"
     CDS             1081..1908
                     /gene="panB"
                     /locus_tag="PC429_003693"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF3849141.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            1927..2775
                     /locus_tag="PC429_003694"
     CDS             1927..2775
                     /locus_tag="PC429_003694"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF3849142.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1134 a          333 c          462 g          901 t
ORIGIN      
        1 tagaattaat tgtcaatttt ttatgaaaat ggtaagatat ttatggatgg tatcttgtta
       61 taagaactat actttaaatg tttttaatta ttttgtctta tgtaactcat atcctcagga
      121 atgagattag aaattaagat gttgtatcac aaagtggagg tgatactatt aaaataggct
      181 ttattggttc tggaaaagtt ggaacatctt tggcaaatta ttttttatca aaaggttatt
      241 gtgtttcagg cttttatgga aggaatcaat tatctttatt agaatcaaca aatttaacaa
      301 aaacaaagat ttacagcaat ttgaaagata taatttatga aaacgatata ttatttatta
      361 caacatcaga tgattctatt gaaattatag acaaaaaact ttccaaattt aatttaaaac
      421 ataaatacat ttgccacaca agtggctctt taaaatctag tatattgttc tgttcaaaaa
      481 aagcaggtgc attaatttat tctatccatc ctatatttgc attttccaat aaaaatacca
      541 acataaaaaa aatgaaagac atttgttttt ccatagaagg tgataatgaa caagatgatt
      601 tagttattca aaatttcata gatgatttgg gaaaccaatt ttttataaga gataaggata
      661 cttcatccac ttatcattta gctaatgttt tagtttcaaa tttagtatta tctttactag
      721 atataggagt aagttacttt attaaattag gtttatctga agaggaatct ttaaaagcta
      781 tcagtccttt agtaaaaaaa aatatagaaa atatacttga taatggcttt actaaagctc
      841 ttactggtcc tgtttctcgt ggagatataa caccaataag aaaacatcta tctgtattgg
      901 ataaaaaaga tgaggatatt tacaaaatac tatccttaaa cctactaaaa ataattgcct
      961 taggaaatga caattcatta aaaggtataa aaaatattac tatagaaaat gcaatcgaaa
     1021 atttaataag taaatctgaa aaatacaaag aaatttacaa acttttagga ggaaaaaacg
     1081 atgaaaaata ccatacagac ttttaagaat gctaaatatg aaggaaaaaa actatctatg
     1141 cttactgctt atgactactc aattgcaaaa ataatggatg agtgtgatat aaatggtatt
     1201 ttaataggag attcattagg aatggttata aaaggtgaag aaaacactct atctgttacc
     1261 atagatgaaa ttatatatca tacaaaagct gtaaaaaatg gtgtaaaaaa tgccttaatc
     1321 gtaagtgata tgccttttct atcttaccat gtttctatag aagatgcagt taaaaacgcg
     1381 ggtagactta taaaagaagg aggagctcat gctgtaaagt tagaaggtgg ctcaaacgtt
     1441 ataaagcaaa tagaaagcat tgtaaatgct caaatacctg taatggggca tttaggatta
     1501 actcctcaat cagtaaattc atttggtggg tttaaggttc aaggaaatac cagtgaaaca
     1561 gcaagacaac ttattgaaga tgctaaatta atagaaaaag caggtgcatt ctctattgtc
     1621 cttgaaggag tgcctacgaa aatagctgag atggtaacta attctatttc aattcctaca
     1681 ataggtatag gggcaggaat taactgtgat ggacaaattt tagtttatca agatatgttg
     1741 ggaatgtttg gagattttgt tcctaagttt gtaaagcagt atgcaaatat aggagatata
     1801 atgaaagatt caattaaaaa ttacatccta gaagttaata caggtacatt tccacaagaa
     1861 aaacacagct tttcaataaa tgaatctgag ttagaaaaat tatactaata ttaaggatgg
     1921 tacaaaatgc ttgtaaaaga aattaaatta ctaagaaata taatcaaaga ttggagaaaa
     1981 catggctatt ctatagggct tgttacaaca atgggatttt tacatgaagg acaccaaagt
     2041 cttataaaaa aagctgtaaa agaaaatgat aaagtagtag tgagtgtatt tgtaaatcca
     2101 actcaatttg gaccaaatga agattttaat agctatccaa gagatataga taaagatttt
     2161 aaatattgta tggatagtgg agcaacagta gtctttaacc catcaccaga agaaatgtat
     2221 ttaaaaggta attgtactac cattaatgtt tctggactta cagattttct ttgtggagct
     2281 aaaagacctg ttcattttgg aggtgtctgc ctagttgttt caaagtttct aaatatagta
     2341 acacctgata aagcttattt tggagagaag gatgcccaac aattagctgt aattaaaaga
     2401 atggtcaagg atttaaacat agatacagaa ataataggct gtcctataat tcgtgaaaat
     2461 gatggattag caaaaagttc gagaaataca tatctctctg aggaagaacg aaaatctgct
     2521 ctaatattaa ataagagctt aagtttagca aaagaagaat tagtaaaagg caatctaaat
     2581 ccagagaata taaaagaatt aataacagct aaaataaatt ctgaacactt agcaaaaata
     2641 gactatgttg aaattgtaga tagtgagaca ttacaacctg ttaagcaaat agaacattct
     2701 atacttgtgg caattgctgt gtttatagga aaaaccagac taattgataa ttttacattt
     2761 gagttaaata tatagtctat aattttatat aatatatcta aaaaataata gaatacctaa
     2821 aaagtaataa
//
LOCUS       DAMKMI010000247         2793 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMKMI010000247 DAMKMI010000000
VERSION     DAMKMI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.253"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..597
                     /locus_tag="PC429_003695"
     CDS             <1..597
                     /locus_tag="PC429_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF3849143.1"
                     /translation="GLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSM
                     TVRENLSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQS
                     LSLIMSSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQ
                     YGDRLIMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            694..1731
                     /locus_tag="PC429_003696"
     CDS             694..1731
                     /locus_tag="PC429_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3849144.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2540..>2793
                     /locus_tag="PC429_003697"
     CDS             2540..>2793
                     /locus_tag="PC429_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3849145.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT         1064 a          360 c          426 g          943 t
ORIGIN      
        1 gggcttataa aagaatcttc tggtcaaatt actttagata gtactactat aacaaattta
       61 gctgagtata aaagaactca aatagtaagt agagtttttc aagacccaag ccttggaact
      121 tgtccttcta tgacggttag agaaaattta tctctagcac ttaataaagg gaaattattg
      181 aacttaaaaa aatgtcttcg tcataaaacg aatgacttgg aacacttact tgaaggaatt
      241 tctttggatt taaaaaaata tttagatatt aaagtacaat atctttcagg gggtcaaaga
      301 caatcactat cactaattat gtctagctta gcaagtccta aggtcttatt actagacgaa
      361 catacagcag cacttgaccc aaaaacatca aatgaagtaa tagagttaac agataaaatc
      421 gtaagagaga aaaatataac tacacttatg gttactcaca acttgaaaca cgctcttcaa
      481 tatggagata ggttgataat gcttcataag ggtgaagttg tattagatgt aaaagataaa
      541 gaaaaaaaag atttaacagt tgaagaaatc ttagaaaaat ttgaatatgc agtataagaa
      601 attaaaatct tagttttaat cagaaatatt tataatctat catatgaata tatgaacaaa
      661 tataattatt aaattttaag ggaggaaaat aaaatgataa aaagtaaaaa aatattaagt
      721 ttaattatag caggagtact tggtgtatca atgcttactg gatgttctca aaatgatggc
      781 tccaatgctt caaatgaaaa taaagaaaca gatagcaaaa aacaaaaaaa tataggtatt
      841 actcaattag tggaacatcc atctctggat aaagcaaaga aaggattcat caaagcactt
      901 gaagataaag gctataaaga tggagataat ataaaaatag atttccaaaa tgcacaaaat
      961 gatatgccta ctacacaaag tattgctagt aagtttgtat ccgacaaaaa agatttgata
     1021 tatgctatat ctactccatc tgcacaagca gcttataatg ctactaaaga tattccaata
     1081 ataatgactg ctgttacaga ccctgtagaa gctggacttg ttaaatctct tgaaaaacca
     1141 ggtggaaatg tttctggtac atctgattat ctttcaattg ataaaacatt agaattggtt
     1201 aaaacattga ctccaaaagc aaagaaaata ggggttatat acaatactag tgaagttaat
     1261 tcaaaaatcc aagttgattc tctacatgat tatgctaaga aaaataatta tgaagtagtt
     1321 gaaaaaggaa tcagctcttc aagtgaagtt aaccaagcta tttctagttt agttggtaaa
     1381 atagatgttt tatatgtccc tactgacaat ttaatagttt cttctatgcc aatagtttct
     1441 aaagttgcta atgaaaacaa gattcctata atagcctctg aagaaggttc tgtatcttct
     1501 ggtgctttag cttgttgtgg aatagattat gaaaaactag gttataaggc tggagaactt
     1561 gctattgaag tattagaagg aaaatctgtt ggcgatatac cagttactac attagatgaa
     1621 actgaaataa taattaacga agatacacta aaagcacttg acatgcaaaa gttatcagca
     1681 gataatataa agtatataaa gtcagatgaa aatgcaaaat ctacaaaata aaacttatta
     1741 atttcaaaaa ataggtaata tgaagtaggt aatatgaaaa ctttatataa taagtactct
     1801 tatccaccct aaccctttga aaattgtgct tattgtagca atttgaatat tgcatataaa
     1861 gttattcatt ttccataaaa gtcgtctata ttgacgactg attatgaaag ttatataact
     1921 ttctaatcac tacttgagct gtccaaaaga attttaatat tctccagaca gctttttttt
     1981 atgatatttg attgatatac atgtctagtt attccatatt tagtaaatat tttgataaaa
     2041 tttccacatt cacttataac ttaggataac gtttttttca aacaatacat taatttaaaa
     2101 gtattctgag tatacattat ttagtatttt aattaaattt agcagttgac ttttattatt
     2161 gtataattta taatttataa caagtcgaga atacatataa ttttctgact aattaaaata
     2221 ttaaaaggga gagataatta tgaatacttt attaaacaat agaaaatatt attattatta
     2281 tagccactat ttttagggtt tgtacctacg ttttaacata gatacagacc ccttaagact
     2341 actaaaattt tgggattgta tctatgttgg ctatattgtt tataaagttt tcatttaact
     2401 atataagagc cacatagatt aaactatgtg gcttattcgt gtttttacca catagttttg
     2461 tacactatgt ggttttttta tacacaaaat tagcaaataa ttaaaattat aaaaatattt
     2521 tcaaaggagt gttttattta tgtcaggtat tatatctgta atgacgcaaa gtttaatttt
     2581 atcgattatg gcactggggg tttatataac ttacaaaatc ttagattttc cagatatgtc
     2641 tgctgatggt agctacacaa tgggtgcttc tattgtagca ttttctctta ctaatggtat
     2701 ttctcctgta gtagcaactt taatggctat cttatgtggt tgtactgctg gtcttgttac
     2761 agggattctt cacattaagt ttaagatatc taa
//
LOCUS       DAMKMI010000248         2760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMKMI010000248 DAMKMI010000000
VERSION     DAMKMI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.254"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            287..790
                     /locus_tag="PC429_003698"
     CDS             287..790
                     /locus_tag="PC429_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF3849146.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            837..1796
                     /gene="moaA"
                     /locus_tag="PC429_003699"
     CDS             837..1796
                     /gene="moaA"
                     /locus_tag="PC429_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF3849147.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            1800..2270
                     /gene="moaC"
                     /locus_tag="PC429_003700"
     CDS             1800..2270
                     /gene="moaC"
                     /locus_tag="PC429_003700"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF3849148.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            2306..2737
                     /locus_tag="PC429_003701"
     CDS             2306..2737
                     /locus_tag="PC429_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF3849149.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         1153 a          253 c          534 g          820 t
ORIGIN      
        1 agagaaaaat atggacaatg ttataataat tatagaaatt aaaagtactt acattcatta
       61 tgtatgtgac atgtataatt agttttatat ttaatacagt tttgaagtaa tatcttcatt
      121 ttgagaacgt tggaataata aaaaatgtat tattataggt gatataaata aaaaataaat
      181 ataatatgtt atattgctga tagaaaagag aataaaagaa aataggtttt attaaaagag
      241 atttttattt ataggactta atcaatgtat agaggggtga gaacatatgt ttaatgttgc
      301 aattataaca cttagtgata aaggatatga aggaaaaaga gaagatataa caggaaaaaa
      361 gttaactgaa tttgttgaaa atacaggagc ttataaagtt actgaatatg tacttataaa
      421 agatgataag gaaatgctaa aggaaaatat tataagactt tgtaatagta ataaaataga
      481 tttaattctt acaaatggag gtactggttt ttcaaaaaga gatataaccc cagaagcaac
      541 taaggaagtg ttggaaaaag aaataccagg tctgagcgaa tatatgagaa tgaaatccac
      601 agaaattact aaaaaggcca tactaagtag gggtgtaagt ggaataagaa ataattctat
      661 aataataaat ttacctggaa gtcctaaagg tgcagttgaa aatctaagtt ttataattga
      721 tgttttagac catggcatag aagttttaag aggagaagct acagaatgtg ccactaaaaa
      781 aagtgaatag tatataatct agatagataa aaaatagaca aagaggtaga taaagtatga
      841 ttgataagta tggaagaaaa atagactacc ttcgtatttc acttacagat aagtgtaatt
      901 taaggtgtgt ttattgtatg ccaccagatg taaaatttga taaaaattat ataaatgaaa
      961 atctaagctt tgaagactac aagtttatta taaaggctat ggctgaacaa ggtataacaa
     1021 aaattagatt tactggagga gaacctcttt tatatcctaa attaaatgag cttataaaat
     1081 ttacaaaaga agaatgtgga ataaataaca taggaatgac cactaatgct attgggcttt
     1141 ctgatagaat ctttgagctt gaaaaatgtg gcttaaataa agttaatata agtttagatt
     1201 ctttgaagga atataagtat aaatccgtaa caagaggagg aaacttaaaa gatgtcttaa
     1261 aatcgattga atcttgttta aacttaggta taaaagtaaa gttaaattgc gttgctataa
     1321 gtgggtttaa tgatgatgag cttaaagatt ttatgttttt aacagttaaa tcaccaattg
     1381 atgtaagatt tatagagctt atgccattag gtgaggctaa tagagtttat aagaagggtt
     1441 attttaatgt aaaagaaatg attgaaaaaa tatatggttt atataaagtc aaaaatgatg
     1501 aaaaaagtac agcagaatat tatatgttta aaggagctaa aggaagaata ggaattataa
     1561 ctccattaag ctgttcattt tgtaacactt gtaatcgtat tagacttact tctagtggtg
     1621 caataaaatt atgtctacat tcaaaagaag aaatagatat aaaaccattt ttacataaac
     1681 cattgttatt tagagaatct atgaaagata taataaaaca aaaaccagaa aaacatcatt
     1741 taatagaaaa taaatgtagt gatacaaata ggcgtatgta tgaaattgga ggataagtaa
     1801 tgttaacaca tataaatgaa aaagggtatg ccaagatggt ggacataagt gaaaaagatg
     1861 atactaaaag aacggccgta gctacagcta caattactct tagtaaggaa gcattagaaa
     1921 aagtaaaaaa tggtcagata aaaaaaggag acgtattgtc tgtagctcaa gttgcaggta
     1981 taatgggagc taaaaataca tcttcaaaca taccaatgtg ccatcagatt aaattagttg
     2041 gatgtgattt agaatttgat gtagatgatt ctatgtctgc tattaagata tacgcaacct
     2101 gtaaatctct tgggaaaact ggagtagaaa tggaagcttt aaatgcatgt tctttagcag
     2161 cacttacaat ttatgatatg tgtaaagcag ttgataaaaa aatggttata agtaatatcc
     2221 atcttgtaga gaagactggt ggaaaatcag gagattttaa gttcgagtaa attaataata
     2281 tattgggtta aaatttggag gaaatatggc gaaggtagtg tcaataaata taagtgagaa
     2341 aaaaggtgta attaaagttc ctgtacaatg tgcagagcta aaaataaatc atgggatagt
     2401 tggagatgca catgcaggaa actggcatcg tcagataagc ttacttggaa aagaaagcat
     2461 agataaaatg aaaatgaaag gctttgacca attaaaatat ggagattttg ctgaaaatgt
     2521 aactacagaa gggatagaag ttttttccct gcctgttggt acaaagttaa gaatagggca
     2581 atgtgaagtt gaagtaactc aaatagggaa aaaatgtcac aatggatgcg aaattaagaa
     2641 attgacaggt gactgtgtga tgccacgaga aggaattttt gtaaaggttc taaaagaagg
     2701 aattataaat gtgaatgatt caatagatgt aatctaatga agtcactatt aaaatataat
//
LOCUS       DAMKMI010000249         2753 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMKMI010000249 DAMKMI010000000
VERSION     DAMKMI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.255"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            162..1382
                     /locus_tag="PC429_003702"
     CDS             162..1382
                     /locus_tag="PC429_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF3849150.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAVVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            1419..>2753
                     /locus_tag="PC429_003703"
     CDS             1419..>2753
                     /locus_tag="PC429_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3849151.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKKGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISR"
BASE COUNT         1044 a          346 c          460 g          903 t
ORIGIN      
        1 gaaaattgaa actatgcaat cattttttat actatacata tagataaatg attatattta
       61 ttcaatattc ttataaaaaa caaaattctc catcgaaaat tatggcataa taattgctta
      121 ttatattaat aaaacaataa aaagcaattg gaggaataaa aatgcaacag gctttgaaat
      181 taaaatatca aaccaaagaa ttaggaaaag attttgtaat agaaagttgt aattctataa
      241 aaccatggct tataaacata agaagagaat tacataaaat tccagaatta gctcttgaag
      301 aaaatcttac taaacagaag gtaatttctt atttaaaaga aatcggaatt gattatatgg
      361 aatttacaaa acacaatgga attatggcat atattttaaa agaaagtgca gataaaacga
      421 tttgtataag agcagatatg gatgctcttc ctattgaaga agaaaacaac ataccttata
      481 aatcaataca ttctggaaaa atgcatgctt gtggccatga tgcacataca accatgctac
      541 ttggagcatg taaagtttta cattcaataa aagataaact taatgtaaat gtaaaattct
      601 tatttcaacc agcagaagaa ggttttggag gagcaaaatt tttagttgag gatgggtgtt
      661 tagaaaaccc caaggccgat tatatattcg gacttcatgt tatgccacat atagaaacag
      721 gccttatcga aacaaaatat gatacattaa atgctagtgt agacactata aaaataagta
      781 tcaaaggtaa aagagctcat ggtgcatatc ctgaaaacgg tattgatgca atagttacag
      841 cttcacaaat tgttacatca ttacaaacta taataagtag aaacttagaa ccaaataatg
      901 ctgttgtttt gactataggt aaaatatatg gtggtgatgc acacaatgta atatgtgaag
      961 atgtaaaatt agaaggcact ttaagaacct taaatagcaa aacaagaaac tttatgattg
     1021 ataaaatagc taaaattgtt ggagatactg catctgcatt tggttgtgtt gggactttac
     1081 atgtaagtga tgaaaactat cctgctgtta taaatgaaaa agaattagta gatacagtta
     1141 tatcaaatac taaagaatta cttggagagg aaaaatttat attgagacca aacccgtctt
     1201 taggtggtga agacttttct ttttacacag aacattgtaa aggagcattc ttccatttag
     1261 gatgtaaaaa tgaggaaaag ggcttaattt cacctcttca tacctctagc tttaatatag
     1321 atgaagattg tttaccaata ggtgttatga tgcatgtaat gaatactttg tattttaatt
     1381 agtacagtaa ttaataaaag aataggagat aaattaatat ggaaaaagct aagaaattag
     1441 gtctatttag tatgatatta ttagggatta atagcataat aggttcaggt atattcttac
     1501 taccaggtaa agtttataat ctagcaggtc aaaacagtat gtttatatac atttttgcta
     1561 cactcttagt tctatcaata cttttatgtt ttgcagaagt tggaagcatg tttgataaga
     1621 aaggaggagc atatctatac tccaaaaaag cttttggaga ttttatagga tttgaagttg
     1681 gtacaatgtc ttgggttatt agaattatat catggtctac tctagcagta ggatttgcaa
     1741 cagctttagg ttcattttgg ccagaatctg ccactgaata caaaggttat atagctgcaa
     1801 tactcgtaac actactttca ataaatagct tatttggaat taaaagcaca aagataatga
     1861 ataatgtaat aactatagct aaattagtac cattaatagt ttttataata gttggaatat
     1921 tttttataaa atttgtaaat attgttcctt caggaaatgt agttaactct agtatgggtc
     1981 cagccattat attagtattt tacgcattta ccggctttga atcatttatt gtggcaagtg
     2041 gagaaatgga aaatcctaaa aaaaatcttc cagtcgcatt aataaccaca atatttatat
     2101 gtgcaataat ttatatatta attcaaattg tctgtatggg tatacttgga gatagactat
     2161 ttgaaaacag tattccaatt gcagatgctt caagtgtatt tgtaggcaat tatggtaaag
     2221 ttttcatatc tgtagcaaca ttaatatcta tatttggtat aaatataggt tcatcaatag
     2281 taactcctaa atgtggttct tctctagcag aagaaggttc tcttcctgca tttataggaa
     2341 aaacaaataa atatggtgct ccatatgtag caataataat atctttaatt tgttgtatac
     2401 cacttgtgct aactggaagt tttgaacaac ttgcagtaat gagcgtaatt gcaagatttg
     2461 ctcagtacat accaacttgt ttatcagtaa tagttttaag aaaaagaaca gatgttaagg
     2521 cctcttttaa gattccattt ggccctgtta taccaatagt agctatttta ggtagcttat
     2581 ggttgttaca acaagcttgg gcagaagata tttcaaaacc tataactcaa aatagagttt
     2641 taataggatt aggggcaatg ttgttaatag ctcctctata tatatttatg aaaaaaaata
     2701 aggaaatcga gcataaaaat aaatcagaag attctaaatt atatatttcg aga
//
LOCUS       DAMKMI010000250         2747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMKMI010000250 DAMKMI010000000
VERSION     DAMKMI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.256"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            26..226
                     /locus_tag="PC429_003704"
     CDS             26..226
                     /locus_tag="PC429_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF3849152.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(390..602)
                     /locus_tag="PC429_003705"
     CDS             complement(390..602)
                     /locus_tag="PC429_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849153.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELKLGEYGVLGWTNEGLYLH"
     gene            977..1159
                     /locus_tag="PC429_003706"
     CDS             977..1159
                     /locus_tag="PC429_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF3849154.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            1230..2075
                     /locus_tag="PC429_003707"
     CDS             1230..2075
                     /locus_tag="PC429_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849155.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNKDPKYNLNTI
                     KKHLN"
     gene            complement(2299..2463)
                     /locus_tag="PC429_003708"
     CDS             complement(2299..2463)
                     /locus_tag="PC429_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849156.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1096 a          342 c          320 g          989 t
ORIGIN      
        1 atataggtta ataggagtat ttttaatgaa taatggaata gtgaaatggt ttaataatga
       61 aaaaggattc ggttttatat caatggaagg tagagatgat gtgtttgctc atttctcagc
      121 tatacaaact tcaggattta aatcattaga agaaggtcaa caagtaagct ttgatatagt
      181 taaaggtgct agaggtcctc aagcagaaaa tataactata ttataatata tagatttttc
      241 gggaaagact ctttttagag tcttttttta ttgttaatat atgtaagata atcattggtg
      301 aagtaaataa ggtatattga gtaatgttct tgatagttta tatgctaatg aatttcaaaa
      361 aaataagcac tcctataaaa agagtgctct taatgtaaat ataaaccttc attggtccag
      421 cctaataccc cgtattctcc aagttttaac tcttttttat atacattatc actttcagcc
      481 atatctaaca caatcatttc ttctttagaa tcaatttgta aaatatgagg actgtgtgtt
      541 gtgacaatta tttgtgtttt tgggagaatt gtttttaatg taataaaaat tttacatacc
      601 acatagttga tataaaactt aagactagca gactcataag caattagttg cctctcttta
      661 cataccacat agttgatata aaacctagag gctctcagag aagttataag atgtttagta
      721 actttacata ccacatagtt gatataaaac tccaaaataa acttagcatc tccaatgttt
      781 acacatatac aatgctctta aaattgcagt gaacgcgcaa tattgcaatt gataatactt
      841 atcatatatt ctcaatacct tatatttcaa ctgctaaacc tcaatttatc acaaatatcg
      901 ctcactgcaa aatttctata tttttattat atcatagcat ataattaata ttttcaatta
      961 atttttacat ttaattatgt ttaaattcta taataatgca actttaatgt ttcttcctgg
     1021 aactaatata aaaactattt cagaaagatt aggtcatgca gatataaaaa taactatgaa
     1081 tagctactct cagtttttag aagagatgga cagagaagca tttgaaaatt tgagcaaaat
     1141 actatttatt gtattttaaa gaagatgact tattcaatat ttgatgctag aatataatta
     1201 ataaaaattt aattataggt gattgtatta tgaatattat taaaagcata actttactct
     1261 taacattatt aattgtgata ttaatgacag gctgttcccc aagagaaagt gctagtgaag
     1321 ttgcaaaaaa atatattgat aagatgaact ctggtgacta tgaaacagca tatagtttat
     1381 taagtaaaga tagtaaaaaa aaaattagta tagaaaatct tgaagaattt caaaatattt
     1441 tagttagtac aaaaagaata attagttata aaataggtaa agaaaaaatc ataaaaaaat
     1501 ataaacatga tggaaaagaa tataaaaatg ttgttaaatt agaagaaact tttaatataa
     1561 aaaaccttct ttataaaaaa gatgattctc taaaagtaaa tagatatttt gtagttgaaa
     1621 ataacaaata caagctatta ttgtatgaaa gtttcaaaaa agaatttggt attaatagta
     1681 tggaccttgc tcaacttaag cttaatgatt ttggactaaa atactttaaa gaagttgacc
     1741 ttatactaaa aaaagctatt gctattaatc ctactgattc aaagctttat tatgcccaag
     1801 catgtaatta tatgaatttt gatggatttc ctggttattc agatacaaca tataatgctt
     1861 tagattcaat taataaatct ttaaagtatt tagataaaaa tgattctgac tacaaaaaaa
     1921 atgcaagtaa tatatataac ttaaaaggag ttattcttat gtttcacaaa agatttgatg
     1981 aagcacaaca atatttaaat aaagccttat actttaacaa agataataaa gacccaaaat
     2041 ataatctaaa tacaataaaa aaacatctaa actagatatt taatttttga atttataaaa
     2101 tattttttta tgtataattc agcactgaat cataatctct actaaactaa ttacaagttt
     2161 aaattataaa gacttatttc tatataaata ttactctata acaataagtt tcttatttaa
     2221 attttaaaat attatctaaa aatcataaaa aagaaggtat aaagagctac cctcaagctc
     2281 aatatacctt ataaatatct aaacttcatt ttttactaat ttttctatat ctttgataat
     2341 atgttcttta gatttttttc cagctatggc atcaaaaatt gcaacagcct ttttctctcc
     2401 aaacttttct gctacatcat taaagtctaa aaatctaaca ctttcttttg gtttaccaaa
     2461 catataacac ctctcataca tttttatata tttattataa cttctataga cttatttttc
     2521 accaataaac gttagacaaa ttagtccctt tattttttta aactagcttt accttcaaaa
     2581 ttcttaacaa aaacagttta ttctactcta taaataaaaa tagacaattt aatttgtagg
     2641 aaactgataa tagaatacag taaataaaac tttgtttaat ctatctgaaa aagttatcta
     2701 ttaaaactgc tatgaattta taaagaatat tctagttcat aaaaata
//
LOCUS       DAMKMI010000251         2708 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMKMI010000251 DAMKMI010000000
VERSION     DAMKMI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.257"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            215..1834
                     /locus_tag="PC429_003709"
     CDS             215..1834
                     /locus_tag="PC429_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF3849157.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2208..2402
                     /locus_tag="PC429_003710"
     CDS             2208..2402
                     /locus_tag="PC429_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF3849158.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
BASE COUNT         1010 a          319 c          378 g         1001 t
ORIGIN      
        1 tctaagttca agtatttaaa ttgctatata tcataaattt tctaaaatta acaacaactt
       61 aaaaatttaa cattctctta acgtatactt aacaaaacat taatattttt attgacaaaa
      121 tttgttattc tatattatgg aaacaataaa ttttagttaa taaagtttat aattcagata
      181 aaaaaattat taatttctta agggaggaaa tatcgtgaat atagtaatta gccttatcgg
      241 tggacttgga ctttttcttt acggaatgag tttaatggga gaaggtttac aaaaatcagc
      301 aggagacaaa ttaaaaaaaa ttattgaact cttaacaagt aatgttgtta tgggtgtact
      361 ggtaggtaca gtcgttacag gtattataca aagttcaagt gctactactg ttatggttgt
      421 aggatttgta aatgcaggta taatgaatct aagtcaagct attggtgtaa taatgggggc
      481 aaatataggt actacagtta ctgctcaatt agtttcattt aatcttgaag gaattgcacc
      541 aattgcttta ggtataggta ttttattcta tttatttaca tcaaatcaaa aaacaaagca
      601 tttatctgag atacttatag gttttggtat attatttaca ggaatggagt ttatgaaaga
      661 tgcagtagct ccacttgctg aatacaaagc attcactgat gcacttttat attttagtaa
      721 aaatcctgta ttaggtatat tggcaggatt tgccataact ggtattgttc aaagttcaag
      781 tgcatctatg ggtatgttaa tagcactagc ttctcaagga atacttcctc tatcatctgc
      841 tttacctatt ctttatggag ataatatagg tacttgtgtt acttcacttt tatcaagtgt
      901 tggtgcaagt agaaatgctc gacgtgcagc tgtaatgcat ttaagtttta atgtaattgg
      961 tacaataata tttatgcttg ttttaaataa acctatatca gccatagtta ctcattttga
     1021 cccaactgat acagctagac aaatagcaaa tgcacatact ttatttaatc ttacaaatgt
     1081 tataatatta ttaccatttt ctaaatacat agttaaatta gcaaatagac ttatacctat
     1141 taaagaaact gaatcagaaa tagtaaataa tactaagtac ttagatgaaa ggatgtttag
     1201 cactccatct atagcacttg gtaatactgt acaagaagtt gtaagaatgg gtcataaagc
     1261 tacaaactct ttggaacatt caattgcagg atttttaaat aagagcaatg aagatattaa
     1321 taaagctttt gagtcagaaa aagttgtcaa taagttacag aaagatatat tgaactattt
     1381 gttaaaactt tctaaagaac ctcttagaga tgatgaacac tttagaacag atttgctttt
     1441 taatactgta aatgacattg aaagagtctc tgaccatgca gaaaatatag ctgaattggc
     1501 catgagtgtt aaggaaatga acattagttt ttctgatagt gcgataagag aaatatacga
     1561 aatatacaat aagacaatta ctaattttaa ggatgcctta atagttttag atgttaaaga
     1621 ttttgagtta gcaaataaag ttttagaagt tgaaaatgaa gtaaattatt tagaaaaaac
     1681 ttttagaaat tcacatatga ttagactaaa taatggaagt tgtacaattg atgctggtgt
     1741 actttatctt gacttactta ctaatctaga aagaatttct gaccactcaa caaatatagt
     1801 aaaacaagta ctaaaactta aacaaaaaat atagtgatta tttttatata taatcttaat
     1861 caatgagaag taagaagcta tagataatgc ttatatcatt tatggtttct tattttttat
     1921 gtaatactca aatttttata gtattctctt attattaaac aatttctgcc tagaatattc
     1981 catatatata tgacttcttt cataaaactt gaactgccca taactaaagt cacaagtata
     2041 cataactatt ttttaatcaa ttataaccaa tatttgtttt atttatcttc attttaattc
     2101 ttcattcaat aatgtaataa gattattttt tatttggata ctattatatc atctaaatat
     2161 gttattatat atttggatat tttcatatta ataggaggga ttctacaatg ataaattata
     2221 tggtagaaat tatatatagc gaaaaaataa gcgaagtaga aaacatgttg aaaattgaaa
     2281 atatacaata tgaatttact tctcgtgatg gctttatatt ttttggtttt tatgatatga
     2341 cagacgcata tagatttgag actttatgtt cagatgaaaa aattcgattt aaaagaatat
     2401 aatagataaa aaagtacata tttctaaact atttgttttt caaatttagg ttgtgtttta
     2461 atgtagtgtt gatattataa acaattttaa taataaaaaa gtagggacat tatattaata
     2521 tgctcctact ttttactcat acttattaat attatttatc aaaattaccc tacaaactgg
     2581 aatttatcat tctttcataa ttttaaacta tcatatttaa aatatataaa attccacatg
     2641 atttttgtat aaacataata tcaaccatta tccaatttaa aaatattttt tagtaatttc
     2701 catttcat
//
LOCUS       DAMKMI010000252         2531 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMKMI010000252 DAMKMI010000000
VERSION     DAMKMI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.258"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            127..861
                     /locus_tag="PC429_003711"
     CDS             127..861
                     /locus_tag="PC429_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF3849159.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(1004..1495)
                     /locus_tag="PC429_003712"
     CDS             complement(1004..1495)
                     /locus_tag="PC429_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF3849160.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1844..2227
                     /locus_tag="PC429_003713"
     CDS             1844..2227
                     /locus_tag="PC429_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849161.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
BASE COUNT          986 a          277 c          370 g          898 t
ORIGIN      
        1 tcatttatat tattaaaaaa tttgtttata gaaactttta ttattttata taaaagtgat
       61 aaaaacttat attttgtatt taatagagtg ttgctaagtg aatatattta aaatggagga
      121 aactttatga aacaaaataa atatgatgat gatagatttt ttaataaata cagtaaaatg
      181 gaacgttcta caaatggatt agcaggtgct ggagagtggc atgttttaaa aaaaatgtta
      241 cctgatttca aaggaaaaag agttttagat ttaggatgtg gatttggatg gcattgtcaa
      301 tatgcagttg agaatggagc aatttctgct gttggagttg atatttcaga aaaaatgtta
      361 aaagaagcta gaaacaaaac gcaacttgat aatataaatt acatatgtat gcctattgaa
      421 gatattaatt ttccaaaaga tagttttgat gtagttatta gttccttggc atttcattat
      481 attcaatcat ttgaagatat atgtaagaat ataagtaatt gtctttcaaa taaaggtaat
      541 tttgtatttt ctgttgaaca tcctatattt actgcatatg gaagtcaaga gtggtactgt
      601 gataaagatg gaaataatct acactggcca gtagatagat attttgaaga aggagtacgt
      661 aggtcaaatt ttctaggaga agaggttata aaatatcata aaacattaac aacttattta
      721 aatgcgttaa tgaaaacagg atttgaaata ttagaaatta ttgaacctca accagaagaa
      781 aaactgttag atacaattcc tgatatgaaa gatgagctac gtagacctat gatgttactt
      841 gtatctgcaa aaaaaagata agtaaatata aataatcatt attgtagaaa aaaagttgta
      901 gaaaataatt acactaatgg agatgattat accagtggta ttaattacac cagtagtaag
      961 gaggcaaaag cattcgttgc ttttgccatt ttttaagttg caattatact tctacgtagt
     1021 atatttcgtt tccattttct tctataagct ttttcatatc ttcaaatttt aaaaatacag
     1081 tagcagtatt atcatttgga tgtataccta ttttatccat ttttaataaa tccttatcaa
     1141 atacaacttt tacaacagcc tctttatcat ttaatatacc aaaaggagtc acagctcctt
     1201 tagtaagttt caagtatttt tgaagtcttt cttctgatgc aaaactaaga ggtttactat
     1261 caatttgttg acgaatatct tttaaatctg cttttttgtc tttacctaat acaactaaaa
     1321 aatgttcttt tcctttagca tcacgaagaa ataggttttt tacaacaaat tccatatgta
     1381 atgtttttaa ttcttccatt tcttcaattg tataaactgg tggatgattt acagcctcat
     1441 aaggtatgct taactcatct aattttttat atacttcctg ttgttgattg ttcatgattt
     1501 cctcccatta gaaatttatt ttatatataa ataatattat cacagaaaac tttaaaagaa
     1561 aacgttttat ataatataga caatagatta tatttttata aagaagcaag aatactcaat
     1621 aaaacttaaa gaaattaaac gatttctaaa aataccttga aaatagaacc ttatttgcca
     1681 tttataaatt atataaaaag ggattatata aagtcatata tgaaaaaact atattatact
     1741 atcttttctt tttggaataa actatacttt aaatatagat atttaattta aataaatttt
     1801 acagtattaa tagtaaatta tcttagaaag gaatgtgctt tatatgtcta cagacaaagt
     1861 tgttggaatc atagatgaag aaacggcaga acttgcagga attgaatata ctggtaaaat
     1921 ttatgcttct tctggtgtta taaaacatat aaagaaaaaa catagatgtc aactgagtaa
     1981 ggatattttt aatgatataa tagacactat aaaaatggta ttaaagtcac ctgaatatat
     2041 aggaagtcat cctaaaaaac ctggaaaaag tgtggagttt ataaaaaaaa ttgatgatta
     2101 tattttagta gctacagaat tagatacaaa aaaaggatat ttatatgtat cttcaatgta
     2161 tgctataaaa gaagcaaaat tagaaagtag agtaaacagt ggaagagtaa aacaatatga
     2221 tggttaacat aatattatta attttcccta aaatattgaa aaatattatt atataagtta
     2281 taatattaat atacacgtag atatttatta gttagatttg aggttggaaa aggcacccaa
     2341 tacgctacta tagagtagtt aatagagatg caggatacgc cgccctgcca aatctaatta
     2401 aattaaaaag agaggattaa ttcctctctt attgtttttt tgagatacta atttaatcta
     2461 taatagaaag attgaaggtc gtttatacaa aaaaattttc agttatatac attaaactaa
     2521 ttaaataaga t
//
LOCUS       DAMKMI010000253         2502 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMKMI010000253 DAMKMI010000000
VERSION     DAMKMI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.259"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            318..2330
                     /locus_tag="PC429_003714"
     CDS             318..2330
                     /locus_tag="PC429_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF3849162.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
BASE COUNT         1108 a          235 c          349 g          810 t
ORIGIN      
        1 atataattta ttttaatata attctatatt tataaaatta tattatcaat agaatttatt
       61 aaaataaatt tttttgggtg tatattataa tttaatcgaa tagaaatgaa aaatatacat
      121 acagtttaag ttttaagcaa caattagggg gatttggtga caagattttt aactataaga
      181 aagaaagtgt cttaatgcaa cttttagaaa gtcattatag acactttctt ttttatttga
      241 taaatttaaa ttatcaaaat tagattgctt tatacaacta ttttgttgga aactatttat
      301 tatataagga gaaataaatg aaaaaatcag tagctctggt aaatgatagt agaaaagatt
      361 taatagactt tttagaaaat aatttgaaat tagtttttgg agattctata aatattaata
      421 gatacttcat aaatgaaata aatgataatg atattataaa tgatgatgtg atattagtaa
      481 tgtcagtaga aagattagac aaaattataa ataatatact agataagaaa aaagtgattg
      541 tggtaagaag aacttttagg gaagataaaa tttataatct actatcttta ccacagggta
      601 caaatgttct aatcgtaaat gattctgatg aaactactct agaaactata agtttatttt
      661 ataaaattgg agtgactaat attagaccaa ttccatatat gaatgataat aactataaaa
      721 atataaaaat agctataact ccaggagttc cagaaaaagt acctagcttt atctcagata
      781 tatttgattt aggtcacaga tatatagata tctctacatt tatagaaata ataaatttat
      841 tacaaattga ttcaaaggaa atacaatcta atctagttaa atactcagaa gaaataataa
      901 gtttagatac tggtataaaa gataaatata aggaattatt tttaaaaata gaagaattag
      961 atacaatctt gaatttatca aaagatggta tattatttac ttcaaaagat ggtgaaataa
     1021 atacatataa tagtaaagta aaagatattt tagacataaa tgaggatata tatggaaaat
     1081 acattgagga tatatttgta gatagtttaa aagttttatt gagtgagaaa gagatactag
     1141 ataaagttgt cgtattcaat aagaaatata taaatgtaaa taaaaaaaac atatacaata
     1201 gagatgaaaa aatgggcaca tattatagtt tacaagaaat aacatacata aaaaaattag
     1261 aacaaaatct cactaaaaaa ttaagagaaa aaggacaaat agcaaaatat acatttaagg
     1321 atataaaaac aaatagtcct aaaatgtttg aatgtataga tttagctaag aaggtttcta
     1381 aatctgattt aagcatactt atcagaggtg aaagtggaac tggtaaagaa ctcatagctc
     1441 agtctataca taacaactca aatagaaaaa atcaaccctt tatagcagta aattgtgcag
     1501 ccgttcctga aaatttatta gaaagccagc tattcggata tgacaaggga acttttactg
     1561 gaggtctaaa agatggaaaa caaggattat ttgaacttgc taataatgga acaatattct
     1621 tagatgaaat aggcgatatg cctttagaat tacaaacaaa actacttaga gttcttcaag
     1681 aaaagcagat aatgcctgtt ggatctcaca atgtaattaa tatagatgtt agaatcatat
     1741 ctgctacaaa taagaattta gaacaaatga ttgataatag ccaatttaga gaggatttat
     1801 attatcgatt aaatacaatt ccaataaata tacctcctct aagagaaaga aaagaagaca
     1861 tattaattat tatggaagat ttaattaata agaaattagt aatcacacca gaagcaaaaa
     1921 aacttataca aaactatatg tggaaaggta atataagaga actccaaaat gttacatcct
     1981 atttaaacat tatgtgtgaa gatattgtat tagagaaaga tttacctcca aacttaaggt
     2041 cttcagataa caaaaataca tcattaaaac taaaatatag taagaatgat atattgaata
     2101 tattagagat actaatttta aataaggaat cagatgttgg tattggtaga ggattaattt
     2161 taaaagcact actcgataag aaccttcaaa ttacagaagg aaaaattaaa aaaatatttg
     2221 aatatctaaa aaaagaagaa cttataattt gtagttcagg aagatatgga agtaaaataa
     2281 ctcaaaaagg agaagatttt tacaataaat taaaatataa aggtctataa aattataatt
     2341 tatcaaacaa agaaatctat tcaggattaa agtactcctt caataggttt ttttattttt
     2401 caagtaccct aaaattataa tttattttgt acataaataa taggaattaa taggaatagg
     2461 ataatttttt tatttcctat tttttttata taaactagca ta
//
LOCUS       DAMKMI010000254         2374 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMKMI010000254 DAMKMI010000000
VERSION     DAMKMI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.260"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            35..1396
                     /locus_tag="PC429_003715"
     CDS             35..1396
                     /locus_tag="PC429_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3849163.1"
                     /translation="MESFIMLDFFITIVSSIIEIWACKKVFDYTSKIKTNSMKLNISF
                     LISIFVIIFTFYLNIDPNIRVLICIISTYSIYKFNYRVNFTKSLIVVFSYWMLLIGID
                     ALAMSLTLFLNNLDSMNLLLEENFYRYESLFISKVFLFFMLFIYKTTFNSVKIGRKEI
                     YLSIPIVSNLLSFIIFYKCIFSFVNISASTKFDIMIMALLLTASNIALIVGIVKMLRD
                     NKKYLELLKLQEKTNLEHNYYQNVKNNYIKTKELYHDMKNHLICIKEMSKNNYDTSNY
                     IKNLEKKLDKYNNMFDTGNIALNIILKEKKELCMKKEIRFITGIDFTQCNFINEEDVC
                     SIFSNAIDNAIEACDKIKEGNKSISLQGRLINNFFAIKISNTKSNTIIEKNKCLITSK
                     KNKEFHGLGIKSIKNTLEKYSGTSTIEFTDTMFALNILIPLNNHYSNNNDFIKMSESK
                     SYD"
     gene            1389..1982
                     /locus_tag="PC429_003716"
     CDS             1389..1982
                     /locus_tag="PC429_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF3849164.1"
                     /translation="MIKFFSDKISYFFSLKQIIHENDLEICSYGLQILFSLCINTIIA
                     LLIGLLLNKVFYTIIFLISFCSIRQFSGGFHARSNRLCIITFISIFSLSMFLGIKLSK
                     LNALPIFVMLISILSFLCIYLLAPVCHINNPLDKNRYKKNKFFSRVISAILLIIIFIG
                     CIYSPIYNCIIYISSALFWVSSLLIIQIFINIKNKEL"
     gene            1984..2121
                     /locus_tag="PC429_003717"
     CDS             1984..2121
                     /locus_tag="PC429_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF3849165.1"
                     /translation="MNKILYKFMKFFGGVAIAIAFISANTTSVWYINQPKMPKSLKKK
                     L"
     gene            complement(2163..>2374)
                     /locus_tag="PC429_003718"
     CDS             complement(2163..>2374)
                     /locus_tag="PC429_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF3849166.1"
                     /translation="ATVVGWGYNDGKILICDIKDYVPGQTQNLYIIGGAACEKIGSMT
                     KEKFTMIKGNDRFDTLYKALDFINR"
BASE COUNT          930 a          273 c          266 g          905 t
ORIGIN      
        1 tattaaatga aatttataca aaattactta agttatggag agttttatta tgttagattt
       61 ttttataaca attgtttctt ctattataga aatttgggca tgcaaaaaag tatttgatta
      121 tacaagtaaa ataaaaacaa attcaatgaa attaaatatt tcctttttaa tctctatttt
      181 tgttattatt tttacttttt atttgaatat tgaccctaat attagagttt taatatgcat
      241 tatttcaacc tacagtattt ataaattcaa ctatagagtc aactttacta aatccttaat
      301 agttgtattt tcatattgga tgttattaat aggaatcgat gcattagcta tgtcgttaac
      361 attgtttcta aataatttgg atagtatgaa ccttttatta gaagaaaatt tttacagata
      421 tgaatcctta tttatatcaa aagtattctt attttttatg ctatttattt ataaaactac
      481 tttcaacagt gtaaaaattg ggagaaaaga gatttattta tctattccaa tagtaagcaa
      541 tttattgtcc tttattatat tctataaatg tattttttca tttgtaaata tttctgcttc
      601 tacaaaattt gatattatga ttatggcatt attgttaaca gcatcaaata tagcattaat
      661 tgttggtata gtaaaaatgt taagagataa taaaaaatat cttgaattat taaaattaca
      721 agaaaaaaca aatcttgaac ataattatta tcaaaatgta aaaaacaatt acataaaaac
      781 aaaagaatta tatcatgata tgaaaaatca tcttatatgt ataaaagaaa tgagtaaaaa
      841 taattatgat acttcaaact acataaaaaa tcttgaaaaa aagcttgata aatataataa
      901 tatgtttgat acaggaaaca tagcacttaa tattatacta aaagaaaaaa aggaactttg
      961 tatgaaaaaa gaaataagat ttataactgg tatagacttt actcaatgta attttataaa
     1021 tgaggaagat gtctgttcta tattttcaaa tgctatagat aatgcaattg aggcttgtga
     1081 taaaataaag gaaggaaata aaagtatttc attacaagga aggttaataa ataatttctt
     1141 tgcaataaaa attagtaata ctaaatcaaa tactattata gaaaaaaata agtgtttaat
     1201 tactagcaag aaaaataaag aatttcatgg actagggata aaaagcataa aaaatacttt
     1261 agaaaaatac tctggaacat ccactataga atttacagat actatgtttg ctttaaatat
     1321 attaatacca cttaataatc actattcgaa taataacgac tttattaaaa tgtcagaaag
     1381 taaatcttat gattaagttt ttttctgaca aaatatccta tttctttagt ttaaaacaaa
     1441 taatacatga aaatgattta gaaatatgtt cctatggatt acaaatccta ttttctctat
     1501 gtataaacac tataatagca ttattaatag gcttattatt aaataaggtt ttttatacta
     1561 taatattttt aatatctttt tgttcaataa gacagttttc tggaggattt catgcaagaa
     1621 gtaacaggtt atgtattatt acttttatta gcattttttc tttatctatg tttttgggaa
     1681 ttaaattaag caaacttaat gctttaccta tatttgtcat gttaatatct attttgagtt
     1741 ttttatgtat ctatttactt gctccagttt gccacattaa taacccattg gataaaaata
     1801 gatataaaaa aaacaaattt ttttctagag ttatatctgc aatcctatta ataataattt
     1861 ttataggttg tatctattct cccatttata actgtattat atatatttcc tcagctttat
     1921 tttgggtgag ttcactattg attatacaaa tctttataaa tataaaaaat aaggagttat
     1981 aatatgaata aaattttata taaatttatg aaattttttg gaggtgtagc tatagctata
     2041 gcctttatat ctgcaaacac aacatcagta tggtacatta accaaccaaa aatgcctaaa
     2101 agcttaaaaa agaaattata aaagaagaag gtaacaactt tctctagttg ttaccttcta
     2161 gtctatctat taataaaatc taatgcttta taaagtgtat caaatctatc attacccttt
     2221 atcatagtaa atttttcttt agtcatagaa ccaatcttct cacatgctgc accaccaatg
     2281 atataaagat tttgcgtctg acctggcacg taatctttta tatcacatat cagtattttc
     2341 ccatcattat aaccccaacc aactacagtt gcag
//
LOCUS       DAMKMI010000255         2371 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMKMI010000255 DAMKMI010000000
VERSION     DAMKMI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.261"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(214..1128)
                     /locus_tag="PC429_003719"
     CDS             complement(214..1128)
                     /locus_tag="PC429_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF3849167.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRYYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            complement(1960..2142)
                     /locus_tag="PC429_003720"
     CDS             complement(1960..2142)
                     /locus_tag="PC429_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849168.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
BASE COUNT          874 a          288 c          292 g          917 t
ORIGIN      
        1 tttaataaat aactgaataa agaaatacct ataaaacata atcactaata ctttttattt
       61 aatataaaat aaagttgtgg gttatagatg attattattt tattttatga ataacttgtt
      121 taaattttat aagtttataa aaaataaagg tacagttaac tgtaccttta ttttatctaa
      181 atatgcaaag attattttaa aagtaatttt taattacatg tttttataac tctccaatat
      241 tctaatataa tgatttgcct ctcttaaaac atggtctgct aaaagtggta atattataga
      301 ttttatctta cactgttcta ttcctgatgc tcctgcttct ttaaaatctt taaactcaac
      361 agtttcattt agagtttctt ctgtaatgtt cttgatatta gaatcagtca tttcgtttgt
      421 tttttcaatt aattcattaa attttatagc aaaatcattt gaagtattta ttagttcacc
      481 ttctgagggg tctagtaatc ctctcataaa cagagcatgt tccatcataa tatggtccca
      541 gaataattct atttctttag cgttttttga ctcaatatct attttatttt caaggtcaac
      601 tacataataa cgatataaat ttgcttcatg tattatatgt tcaagaagta gagggtagtt
      661 tgatgtaaat atagtacatg atagtacacc atctaagact ctttctttaa aattaataag
      721 cccatcaagt aatcttattg catcagagtt aagttgagct acatagttca ctaagtcttg
      781 accaacttgt gggtttacac cactttgtag atttaattct cttgtagtga tgtttttgtt
      841 tatttctatc cctgtaaact cttctgtttt ttgttctgca actgatgtga gagtagttac
      901 aagctcttct gaatataata tatcaggtct aattatacca ttactagcac taacagtgta
      961 tgataataaa tcttcaaatt gctttttata gtggtcagct tccatagcaa gattataatt
     1021 tttatttgtg aatcctgctt ctaaaaaaag agcatgctct ttcataattc ttgaaaaaaa
     1081 taaatgtaat tctagtgata aaataacata tttttgatta tctatcataa tttcctccct
     1141 ttagaaagta tcctatttat aatactttat tcgtaatatt taaatttatg tatattataa
     1201 ataaattaac taaattcaat tgctactaat atcaacagtt tttattaagt tatgggcgaa
     1261 aaaattatct tactgtgttt cttattagat atagatataa tgatttaagg tacttgttgg
     1321 ctattattta ttaagagtac aagtaatact agaaattttt atgagaatta tgtaatttac
     1381 aagtattatt ttgatacaaa acaaatgttt tttgtaaaac aagaactttt atagtaaaaa
     1441 atgaataaat atgaagcaag cataatttta taccacaaca attattaatt ctaatagctg
     1501 cacttaatgc tttaggtatg ggatttaaaa agtataaaaa attaagtgat aaatatattc
     1561 cattatattg cttgtgcttg gagtagtatt ttctgtatag atgttaggat ttaatccaac
     1621 ttctatttta caggggattc tatgctgggg tgtttcaatt ggattaaatt aagtatgcaa
     1681 acaatttcaa gacaatgata aataattgga tgtgtgatag gaatatcaac aaaaaacatt
     1741 attaaaaaga attattttat ttataaaata aaacaagtaa taagaataaa taaggagcaa
     1801 ctaaaatgat ttaaatatac tagaattaaa aagagaatgt aaagttgtct agtaatggat
     1861 gtaaaatatt ttaaacgata agctatgaaa ttgatatatt aaaatgggta agaagaatat
     1921 tatataggat tcttcttacc cattttaata aatttaattt tatgccaaac cacattcgca
     1981 tagtagaatc atcactctat ggagacataa ctctacaaca tcttctccat attttttaat
     2041 aaaatcacca atttgagttt ctatgaatga atagtattct tttaaactag caatatatgc
     2101 agtcattata tgaacacatt catcaatctt atctttaaac acaactaatc cccctaaaaa
     2161 acgaaaaata aaaatattca gtaaatagca gatattattt cactcataaa tctatatagt
     2221 atgatatatt gcttagatat attttacaag tggatatgta aaactggtta ttataatcgc
     2281 aaaactggat aaactttcat tcttaaaaga aaatttatcc agtttaaagt ttaattatga
     2341 atatttgagt tagtagatat ttaatataaa t
//
LOCUS       DAMKMI010000256         2370 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMKMI010000256 DAMKMI010000000
VERSION     DAMKMI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.262"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            925..2280
                     /locus_tag="PC429_003721"
     CDS             925..2280
                     /locus_tag="PC429_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF3849169.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          841 a          255 c          401 g          873 t
ORIGIN      
        1 tatatttgag caaataagta aagtaataaa aaaatcaaga tttcatatct aaaaacaatt
       61 ctgaacatta atttaattta tattttcata ggaaatattt tatataatat acttgataaa
      121 tataatttga atttttctaa ttatttactg tcgattaata gatttttttc ttgcatataa
      181 gtgcatattt atggtacgat ttacatggtg aaataaaaaa ggggaggtga aaaataaata
      241 ttcgtgaaaa agttttcagt ttgaggtatt ttatgaataa tatattttga ggtattttat
      301 aaataatata ttatacataa agagtataac ctcaataaat agtatcaaag cgaatatatg
      361 ggtaattact ttaaaataac agtttttatg tttagattca cagtgtttat tataaactaa
      421 tgtgtaagaa ataagcctaa tctgtgaata gatagtagtt tagttattta agttgagaat
      481 tggttgagtg aagtaatata taaatttaaa aaataaataa aaacattatt tgaacaaaat
      541 atatgttgct ttattcgaga ctcctttaaa aaagcactaa aatgtaatta cataagttgc
      601 tacaaaaggg ggattttagt ttgtaaaata ctatcacgta aaaaataaga attgcatatg
      661 gcgaaggttt tttcatggta ttagatgaga atatattaat ttataactat atctgaaaat
      721 agtgaattta tataatttaa atttatgatt aagaagtgta ttaattttat tccatgagaa
      781 tatgtactct acaaataaat gcgcaattat cttaataata attacaaatt gttttacaaa
      841 ataataatat tatatctttc ataaaagaaa gaatttggac aaaacgtaaa aaataaaaat
      901 acaattaaga aaggatttaa aattatgagc tcaacagtaa aaaagaaggg taaatttcct
      961 atgggatttt atatctgctc aacaactttc tcatttgaaa gagcagcata ttatgcttca
     1021 aagtacttaa tatatatatt ccttacaaca gctatagttc atggtggtct tggaatagat
     1081 aaaggacaag ctgctataat gcaagccaac cttgttgcat tcacatacct tgcacctata
     1141 attgggggat atatatcaga tagatggata ggagctagat atactatacc tataggtatg
     1201 cttatcatgg gagtaggata ctatcttggt tctattgcaa ctacagtttc tatggtaaat
     1261 gcaatgataa tcttagtttc aattggtact gcattcttta aaggtaacgt atctgcggta
     1321 aatggacagc tttttgatag tcaagaagaa cttgatacag cattttcagt acagtattct
     1381 tttgttaata taggttcttt tattggtact atagcagttg gtattcttta ccttaaaaca
     1441 tttgcaaaaa atggtgtact tggattttct caatgtttct ttatagcagc tgtactttgt
     1501 gtaataggtg caatatggtt catatatgga tggagattcc ttgggaatgc aggaaagaga
     1561 ccttttaaag aaggtgtagt tgctgaaaaa attgaagaaa aagataagtc tcctcttaca
     1621 agcatggata agaagagaat ttgggctatt atacttatat cattcttctc tgtaattttc
     1681 tgggtattct ggtatctaac ttatcttgca gtttatgatt atggtgcagc atttgtaaac
     1741 atgaatgttg gaggatttga tgttcctctt gcttggtttg actcattaaa ttcacttgta
     1801 tgtatagtat taggaccagt tcttggagca ttatggttta aacttgctag tagaccacaa
     1861 ggagatatga gtttatttaa aaagacaggt cttggattaa tattcttagg tcttgcattc
     1921 ttaatgcttg ttggtgcaga attttcaaga ggggttggag ctcctgaaac tgctaaagct
     1981 agtatactat ggataatcat gtttggtatt cttttaagtc ttggtgaaat gttattctca
     2041 ccacttggta actcttttgt aagtaagtat gcacctaaga agcttctagg tgttcttatg
     2101 ggagtatgga catttgctac ttttatagct ggtaaaggat acggatatat atatgcattt
     2161 actcttaaat ttgatatgat taaagtatat actataattc cagtaatact atttgttgca
     2221 gcgatattat tattcttatc tgataagaca ttaagtagac ttgttgagga tgataaataa
     2281 ttagtaagca attattatgt tttataggtt aatctactgt aattatgttg tattagattg
     2341 atttataact aattaataat aaaaataaaa
//
LOCUS       DAMKMI010000257         2346 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMKMI010000257 DAMKMI010000000
VERSION     DAMKMI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.263"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            138..278
                     /locus_tag="PC429_003722"
     CDS             138..278
                     /locus_tag="PC429_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849170.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(275..646)
                     /locus_tag="PC429_003723"
     CDS             complement(275..646)
                     /locus_tag="PC429_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3849171.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1195..1452
                     /locus_tag="PC429_003724"
     CDS             1195..1452
                     /locus_tag="PC429_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849172.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1562..2242)
                     /locus_tag="PC429_003725"
     CDS             complement(1562..2242)
                     /locus_tag="PC429_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF3849173.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          946 a          263 c          267 g          870 t
ORIGIN      
        1 gataattatc tagggaaatt ttggataatc aaaatatatt ttataaaatc aaaatacaat
       61 tacataaact ttattaaaaa tttgttggga atttctgact aacattaaga acgtaagttt
      121 gttgggggat ggtatatgtg gaaaagtcaa acactactgt tacaaaaaaa gaaaaaattg
      181 aagatatagg aatgaaatta gaacatttat tgagtgaaga tattactttt tatgaattat
      241 tacttgagat tttgtataaa gaaaaagaag agaattaatt ctcttcttta aggaatcttt
      301 ttaaataggc attatcaatc attttttcta tagcttctct atcttctttt tcaagagaat
      361 atattttttc taagagcttt tttgtttttt cacttactga taagttctct atgattaatt
      421 tatcttcttt gcttattttt gtattatcat ttacgcctaa taaataatct gttgatgttt
      481 ctaatgcttt tgctaatcta actattattt cacttttagg ttctcttaaa ttattttcat
      541 aacgtgacaa gctagcctca gttataccga ctttagtagc aagttctttt tgatttatat
      601 ctaattcttt tcgtagattt gctattctat ctcctaaact ttccatatgt acatcactcc
      661 ttaccgaaat tataagttat tttgataaaa agtaaaataa aattaccaaa aatataaaaa
      721 aaaaaggtta aaataataaa atatattgaa aattaccatt tgtatatgta taatatgaat
      781 tatacaaatg gtaatttgat gagaggtggt atgttgaaat aagaaactga attaattaat
      841 aaatacatag aaagttttga aaacctttaa ttgaaatata aacaatatgt acaaaatttt
      901 ttgcaagtaa aattaccaaa tgtataaaat aaatcagtag catagataat tttatgcttt
      961 aatacattag attatttaat ttagttataa tggtcaaaaa tgcaatctac aaataaaaca
     1021 aagtattatc aaaaaagtat tctttaagaa aattttttat tagagatgta attgatataa
     1081 ataatcgtat taaaaaataa aatactaagt ttaaaaattt aaataatcta taaatcaatt
     1141 ataaaatatt atttcttaga aattaaaatg aattaagtaa atggaggaat tgatatgata
     1201 ataattagaa gtcaagataa aactaattta atgcagataa accaaattaa aatagatggt
     1261 agtcaagttt atgcagtatt tgaaagtaaa attgatactg taaaaatagg agattatgaa
     1321 aataatacaa gagctataca agtgttggac aatatacaaa acttcattga aaatggaaca
     1381 aaatatgatt atataacatc aaataaagtc agatacaatg ttaataaaat atttcagatg
     1441 ccagctaaat agtgagtgtg cattttaatt taataactaa agcccacttt cttttgtggt
     1501 atgaaagagg gctttacata tttgtaaata tcgaagtaca cttgttttat caataaatta
     1561 tttaataagg tataatggac caattaaaca ataaaaattc tcctaaaaca gcaagtatta
     1621 aatatataat agcttcattt ttgtaaagag aggtaatttt ttcgcttatg tcatcatcaa
     1681 ttgtaatagc aatttcatct tgaataatat ttttatatat agatgggaaa atatcttttt
     1741 tctttataag attaaataaa ttatttacac ctacccatat aaaatatcct aaaacagcac
     1801 ctaatgtgtt catcattaaa tcatcaatat ctgtagctct taagttaaac aattgacaaa
     1861 gttcaattgc taatgaaaaa cctaatccag ttaatgaaac attgataata tttctaaaat
     1921 ttttccaaat taaaggtagt aaaaatccta aaggcataaa cataattata tttaaaatat
     1981 atgttaagat gccaactgag tcaaaaggta ttagatttat ttcatcaatt ctaaaaagcc
     2041 cactatacct tcctatatcc catatgctac caattccagc tttactaagt gctagataga
     2101 tatataataa aaaaatatat acccatacta aatgtatcat tacattcttt tcaactttca
     2161 aattttttcg ttttataacg actatttggt atattaagca tggtaatata acgcaaataa
     2221 atgaataaaa tgtccatatc attttatttt gtcccctttc aaatttatgt ttttagtttt
     2281 ataagtacta tctttatatt aaactgtaag ttttaaaaat cttttaataa aatcttaaaa
     2341 atttat
//
LOCUS       DAMKMI010000258         2288 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMKMI010000258 DAMKMI010000000
VERSION     DAMKMI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.264"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            330..2225
                     /locus_tag="PC429_003726"
     CDS             330..2225
                     /locus_tag="PC429_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF3849174.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
BASE COUNT          999 a          235 c          427 g          627 t
ORIGIN      
        1 agatagatat taatttagtg atatatattt aagtaataag agtaaagcaa aaattagtgc
       61 atagtagtat ataaaaatgg agtttgaata cttttgttag tatatgattg tttagtagaa
      121 aatcactata aaataggatt tctatctttg agcataatta tagagagcct aagtaggggg
      181 aagggtttca agaagatatt attagtgttt gtgttagtaa attatgtaaa cacataaaat
      241 atattgaaaa ataatattat atataatatt atttatgtgt gggaaatacg aatataaaaa
      301 ataaaaaata atattcggag gacataaata tgttaagcaa caaaaagaga agtatggcaa
      361 tagtaatggc aggtgctaca gttatgagtg cagcagcacc aatattcgca gacaataccg
      421 tgacagaaaa tgttgataaa aattacacag taagtgcgaa agattctgct aaactaatcg
      481 aagaggttag aaaagcatta gaagttaagt ttgaagatac aaaagcagga gcaaatgtaa
      541 atgatagagt ttacgatata aaagtagaca atgtaaactt aactaatgca actcaattac
      601 aaaataaaat aaactcttta acagaagggc aaagtttaaa agttactatt caagataaag
      661 ggcatcaagt gttaggtgga aaagtagttg actataaaat tgaaaactac aaaactgctc
      721 aagaaatagt agatgcagtt aatgcttaca atgcaacttt agcagaagat tctgataaca
      781 agttaacagc aactataaaa tctacaaata cagttgaagt aaaaagagca aaagattcag
      841 caaatgttat tacattaaat gtaggagacc aacatttaga tttctcaaaa gtgataacat
      901 cagaagaagg tacttttgaa ggttatgaaa agagttatag tgacatagat tcaaaagaat
      961 tacatacgat aacagttaaa aacgcagatt tacaagacat atcagcagaa gaattatttg
     1021 atggaattag attaactaca ttaggaagag aaatagttaa taaagttaaa aatggatatg
     1081 ctttaacatt tgaaaatgaa gcaatcctta ctcaagaaca agaagatagt gatgataaag
     1141 ataaaccaga aaaatcaagc tttgatatag ttttaagcaa ggcaaatgaa aagccagaaa
     1201 caataagtgt ttcaagtaaa aaccataagt tagttagaga cttacacaaa gtattaactg
     1261 atgtaaaaga tggaaaagaa ttaaaagtag aagttttatc tggtgattca agatttacaa
     1321 cagcagtaga agttagtaaa gagagattta aagatggaga agcagaagct ataattttag
     1381 ttggagaaga tgctatagtt gatggattag catcagcacc acttgcctct caaaaaaatg
     1441 caccaatatt attatctaaa aaagattcac taccatcaga aatagaagct gaaatattaa
     1501 gagtacttgg aagtaatcta tcttctaaga aaatatatat agtaggtgga gaatctaaag
     1561 tatcaaaaga aactgaagaa aaactttcta aattaggtgt aagtaaagtt gagagagttt
     1621 ctggagaaga tagatttgaa acttctttag aaatagcaaa acaattaaaa gatacattta
     1681 agactgcttt tgtagtaggt ggaaatggag aagctgatgc tatgagtata tcagctagag
     1741 ctgctcaatt tggtgctcca ataatagtta caggtaacga attagatgca aatgctgaaa
     1801 aattattaaa aggaaaagaa ttagaaatag tgggtggaga aaattctgta tcaaaagaag
     1861 ttgaagacaa attagttgat atagatttaa ataataaagt tgaaagatta gctggagaaa
     1921 atagaaaaga tactaatgct aaagtaatca ataaatacta tgcaggtgca actaaagcat
     1981 atgtagcaaa agatggttat gtaggtggaa atggacaatt agttgatgca cttacagcag
     2041 caccacttgc agctagttca aaggctccaa tagtattaac tacagaagaa ctttctaaat
     2101 cacaagaaga agtagttgag ttaagactta agaatgctac taagttagta caaataggtg
     2161 aaggaatagc taaaaacgct attgaaaaaa tagcagaaaa aataaactta tttactaaga
     2221 actaataaga tataggaatt aatattaaaa gtttatttag ataatatatg attatacaat
     2281 gcgttaat
//
LOCUS       DAMKMI010000259         2256 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMKMI010000259 DAMKMI010000000
VERSION     DAMKMI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.265"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(133..1425)
                     /gene="brnQ"
                     /locus_tag="PC429_003727"
     CDS             complement(133..1425)
                     /gene="brnQ"
                     /locus_tag="PC429_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF3849175.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGIK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          755 a          372 c          246 g          883 t
ORIGIN      
        1 aatttttaaa tttatcaaat aggattaata atgttctttt atctacaata atttaataat
       61 tcttttaaat ataaaaagga attgcctata taatttaaag gcaattccat aattatcaat
      121 tagtttttat tattatgcag ttttactgct tttagatgga ataaagtttc ctattattcc
      181 tgctattaca actggaacta tccagttaaa tcctaatgaa gcaaatggta agctattaac
      241 tagaggaatt tttataccaa aacttgttgc aactgttaat acacttacta ataatgccat
      301 gtatgcagca catttgaaca cgttgtcatt tttaatttta ttactaaata atgctagtat
      361 aactagagtt atagttgctg gatataccat acttagtata ggagaagaga attgtattat
      421 tgtacttact ccaaagtttg atatgattgc actaaataca caaactacta taactatatg
      481 ctcatacttt aattttccat ctgtaagtgt tgagaaatat tgaccagctg ctgaagaaag
      541 acctatagct gttgttaaac atgctaatgc aactataata gccaataaaa ttttacctgt
      601 agttccaagt aaagctgctg ttattgcaac tattaaagct gtttgttgaa catcttgtcc
      661 atattgttga gacacagttg ctccaagata acataatcca ccataaacta atgccattcc
      721 tattactgca attacacctg cacttattgt ggctttcatc ttttctttct cacctgtata
      781 acctttagct attactgaca tgattataat tgatgcaaat acactagctg ccaatgcatc
      841 cattgtttga taaccttgag ttataccttc tgcaaataca ttttgtatta aagaactgtc
      901 tcttatttct cctaatggag atacgatacc tttaactatt aatactgcta aagctataag
      961 taaagctgga gttaatattg acccaactat atctacaact tttgatggtc ttattgttaa
     1021 aaccaatact agagcaaaga atactataga gaatattact gggcttattc cagaacctat
     1081 tgtaggtaat atacccattt cataagttgt tgctgctgtt ctaggtattg ctaaaagtgg
     1141 ccctatacag attaccattg cacatcctaa tgcaatactt aatcctttgc ctgctcttga
     1201 gaacatcttc gatgtgtctc cattgaactt agttgctgct ataactgcta ataatgccaa
     1261 accaccatct gcaaaaagga atcctatgaa agctactagc caactacttc cagataaaac
     1321 tcctagatat ggagggaaga ttaagttccc tgcaccaaaa aacattgcaa atagtgcaaa
     1381 tccaattaca attacatctt taaaattgat ttgtttctca cccatttaaa gcacccccat
     1441 ttgaatttta ctacctttta ctaaaaaata agtccaatct cttaattaaa tgaaaacctt
     1501 ttgacgagat tttatttaat tatacatttt tgacttaaat ttgtcaatta tgtttcataa
     1561 tttataaaat cttcacaata tttaatacaa ctttcttaat taaacaaaat caacctgtat
     1621 actttaaaca tagttggttt tttgtttttt ctctaatttt tcttgataaa attaactatt
     1681 ccgaataatt aaaatattaa gactttttag attattcgat ttattattaa gttgtattgc
     1741 tttatatact gtatatatca ctctcctttt ttacattcat ataatctttt taattcaatt
     1801 taaattttaa atattttgtg tttattatat aatctgaaaa aaaagcagtc aatagcattt
     1861 ttaatttttt taattttcaa aattatgatt cttaaaaaat cgttttcctt ttatcataac
     1921 acctatttac gttaattctt gtagtatttt gtatttattt tgctaaattg acagttaact
     1981 ttattcaatt tttattctac ttacgtaaat ttgcctcttg ttttttactc ttaaaaccat
     2041 tttttctgaa aattccttat tatgatttat caacttattt ttcttcataa caattactct
     2101 ctaacttcca acttacttaa actttgcagt tgctagtatt cacgtttaca tacttatatg
     2161 tcataattaa ctaaaaattc ttaatatatc tctcaatgaa aacaatttac tcatttattt
     2221 aatacattta tataatcata tgtcttaaat tttgtt
//
LOCUS       DAMKMI010000260         2105 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMKMI010000260 DAMKMI010000000
VERSION     DAMKMI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.266"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            133..>2105
                     /locus_tag="PC429_003728"
     CDS             133..>2105
                     /locus_tag="PC429_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF3849176.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLET"
BASE COUNT          919 a          164 c          350 g          672 t
ORIGIN      
        1 aataaaataa aaacaggtat aaaaaggtat ataataaggc tttcatatac atttatgagt
       61 caaaattttg atataatata tagatacatg gagttatgaa tggaatttat ttaacttata
      121 gggggagtta atatgaaagc aggagaaatt gaatttctta gttatttaga ggggtcaaac
      181 aaaagttttg tagtacctgt atatcaaaga aattataact ggaagaaaga acaatgtaaa
      241 agattgtttg atgatttaga ggatataata gattctggat ttagaactca tttcttaggg
      301 tcagttgtat ctatatatag tatgggaaaa gaatatttaa taatagatgg acaacaaaga
      361 gtgactactg tgtcaatatt attgattgcc atgtgtcaaa taataaaaga gtttggggaa
      421 tcaaaaggaa ttattacaac agaagaacag attaaggagt gttatcttat aaacaagcac
      481 tcaagagata aagatggaaa aataaagtta aaaccaatag cagaagatag aaaagcgtac
      541 tatgaactat ttgaagaaga aatgaataca gatagaaatt ctaatatagt ttcaaattat
      601 ttatattttt ataatagaat aaaagaagat aatatatcaa tagatgattt gtttgatgct
      661 atacagaaat taactatagt agaaatagag ttaaaaaatg gtgaagatga ccctcaatta
      721 atctttgaaa gtctaaattc aactggctta gatttaagtg aagctgatag agtaagaaac
      781 tttgtgttaa tgggtagaag ctctaagtta caagaggaat tttataataa ttattggtat
      841 aaaattgaaa aaaatacaag tagcaacgta agtagtttta taagagacta tttgactata
      901 aaggagagaa gtataccgaa taaaaataaa gtatatttta gttttaaaag gtatgtatca
      961 gataatggta tagatataga aattttatta aaagatttac ttaaattttc taactattat
     1021 agagagatat cttattcatg tactgatgat aatgatgtaa acaaagtttt gaaaaggata
     1081 aacaaacttg aaatatttgt atcatatccg tttatattag aattatttga tgattataat
     1141 gaaggtataa taaataaaaa agtacttgta gaatccctta atttagtaga atgttttata
     1201 ttaagaagaa tagtatgtga tgtacctcct aatgcactga ataaagtatt tatgaactta
     1261 ggaagggaaa ttaaaaaatt tagtgattat aaagaaaact attctaatat attaagatat
     1321 atttttataa ataagaaatc atctcaaaga tttcctactg acagtgaatt ttcaaatgct
     1381 tttgaaacta aagatatata taatactaag tcaaagaata agttatatat acttgagagg
     1441 ttagagaatt ataataataa agaaattgtt gatttagaaa atttaatcaa taataatatg
     1501 ctaaatatag agcatataat gccacagaca ttaacaaata aatggaagga atcactagga
     1561 gataactata aggagataca tgataaatat ttacatacaa ttggaaattt aactttgaca
     1621 ggatataatt caaaactaag taataaaaca tttgaagaaa agaaagaaat ggaagatgga
     1681 tttaaagata gtaggcttta tctaaataaa tatatttcat ctatagataa atggaatgaa
     1741 gaagaaataa aaaatagaac taagatatta ctagatagag ctgtagaaat ttggaaatat
     1801 ccaagtgtta catatgaacc tccaagtaac agtgaaaata tatttacgtt agaggatgat
     1861 gatgttaatt ttacaaatga aagtatagta tcatttagta tattaggaga gtcatataag
     1921 gctaaaaatt ggacagaatt ttatgaacaa gttgctctta ctttatatga tttggattct
     1981 gtaaggttta acagtattgt agacaagact tataaaagag attttataga taatattaag
     2041 tctagagatg catataaatt aagaaaaggt attaaaatat cagaaaatat atatttagaa
     2101 acaaa
//
LOCUS       DAMKMI010000261         2043 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMKMI010000261 DAMKMI010000000
VERSION     DAMKMI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.267"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            117..974
                     /locus_tag="PC429_003729"
     CDS             117..974
                     /locus_tag="PC429_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3849177.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            1071..1940
                     /locus_tag="PC429_003730"
     CDS             1071..1940
                     /locus_tag="PC429_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF3849178.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT          844 a          200 c          368 g          631 t
ORIGIN      
        1 tatatttatg aattttatat gaaaatagtt aaaatattga tttttttcat tggttgaaaa
       61 aacaatttac ttaggataaa ctataatagt attcaaatat tatgggggtt agatttatga
      121 gtaaagatgt aaaggtaaag cgtaataaga aatatgatac atttagtata actatggaac
      181 ttgagagacc tttactttat gatgaggttg agaacaaaga tgataaaaat aataatagaa
      241 aatttaacat aaagaataag atatttaagg gaataggtat actatttgta ttgattttgc
      301 ttggcttttt catatggata aataaaacat atgaacctca aaagctggca aaagaagctc
      361 tagtcagcaa tagtaaagtt gaagtaactg taaatgaaaa catttcattt acaccaaaag
      421 gaagaaaagt ttctaaaggt ttaattttgt atccaggagc taaagtagaa atagaatctt
      481 atgctccact tgcaagaaaa atagcagaaa gtggatatga ggttgtaatc gtaaagatgc
      541 cacttaatct agctatactt ggaactaata aggcacagaa agttatggat tcttacaaca
      601 acatagaaca ttgggtaata ggaggtcatt cattaggagg agttgcagca tcaaattttg
      661 cacgtgataa taaattaatt gaaggcgttg tatttttagc ttcttatcca atgggtgatg
      721 agttgaaaga attaggaaaa aaagttatat ctatatgggg gagcaaagat ggagtagtca
      781 attttaaaag tcttgtagaa tcaaaacaaa aacttcctga tgatacaact tatgttgaga
      841 ttgaaggagg aaatcatgca caatttggag attatggaaa acaaaaggga gataatgatg
      901 ctattataag tcaagaaaaa caacttaata ttactgccaa tagtatcatt aaatttttaa
      961 agaatatatc ttaaactaaa atttaaaaaa tataatttta taagttatta tttaaagtaa
     1021 atattaataa tataatagaa tgaatatttt attacaaggg ggattttaat atggaatacg
     1081 atatgccact ttacagacca ccaagtgagg cttatagtct tataatacaa gtcacactag
     1141 gatgttctca taacaaatgt acgttttgta gtatgtacaa atctaaaaaa tttaaaataa
     1201 agccactaga agtaataaaa aatgaaattg atatatttag aagatattat aagcatgtag
     1261 aaagaatatt cttagcagat ggagatgcat tgataatacc aatggagaaa ttaagagata
     1321 ttatcttata tataaaagaa gtttttccag agtgtgaaag aattactatg tacggctctc
     1381 ctaaatctat agagaaaaaa tctgatgatg aacttaaaga attaagaaaa cttggtgtta
     1441 aaatgattta tctagggctt gaaagtggaa gcaatgaagt attagaagat attaagaaag
     1501 gttttagtag tgaacaatta ataaaagttg gtagaaaagt taaaaaaagt gggattaaat
     1561 tatcagccac tgtaatagca ggtcttggag gaactaaaaa aacacatcag catgcagttg
     1621 atacaggaaa aatgttagga gcaatttcac cagattatgt gggtgtactt agtttaatgg
     1681 tagaacctaa tactgaactt tacgatttac ttaaggcagg agagtttaca atactagagg
     1741 ataaggctgt gttacaagaa attaaagaga tgataaagaa tatagatgct aatgaaaaaa
     1801 ttgtatttag aagtaatcat gcatcaaatt atgctaattt aaaaggtgta ttacctgatg
     1861 ataaacaacg tctaattgat gaaattgatt actatctaag taaccaaaag ctaaaaagag
     1921 aagaatatag aagactatag tttttgatat atcaatctat attgataggt aaatactaag
     1981 attatttaga tacaaaaaat catgctacat taatagttgc ttactattga tgtagcatga
     2041 ttt
//
LOCUS       DAMKMI010000262         1986 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMKMI010000262 DAMKMI010000000
VERSION     DAMKMI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.268"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            84..200
                     /gene="rrf"
                     /locus_tag="PC429_003731"
     rRNA            84..200
                     /gene="rrf"
                     /locus_tag="PC429_003731"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            207..281
                     /locus_tag="PC429_003732"
     tRNA            207..281
                     /locus_tag="PC429_003732"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:239..241,aa:Asn,seq:gtt)
     gene            286..360
                     /locus_tag="PC429_003733"
     tRNA            286..360
                     /locus_tag="PC429_003733"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:319..321,aa:Glu,seq:ttc)
     gene            366..441
                     /locus_tag="PC429_003734"
     tRNA            366..441
                     /locus_tag="PC429_003734"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Val,seq:tac)
     gene            447..523
                     /locus_tag="PC429_003735"
     tRNA            447..523
                     /locus_tag="PC429_003735"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:481..483,aa:Asp,seq:gtc)
     gene            534..608
                     /locus_tag="PC429_003736"
     tRNA            534..608
                     /locus_tag="PC429_003736"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:566..568,aa:Thr,seq:tgt)
     gene            623..707
                     /locus_tag="PC429_003737"
     tRNA            623..707
                     /locus_tag="PC429_003737"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Tyr,seq:gta)
     gene            717..790
                     /locus_tag="PC429_003738"
     tRNA            717..790
                     /locus_tag="PC429_003738"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:749..751,aa:Gly,seq:tcc)
     gene            801..877
                     /locus_tag="PC429_003739"
     tRNA            801..877
                     /locus_tag="PC429_003739"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Arg,seq:tct)
     gene            887..962
                     /locus_tag="PC429_003740"
     tRNA            887..962
                     /locus_tag="PC429_003740"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:920..922,aa:Gln,seq:ttg)
     gene            974..1049
                     /locus_tag="PC429_003741"
     tRNA            974..1049
                     /locus_tag="PC429_003741"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Lys,seq:ttt)
     gene            1052..1140
                     /locus_tag="PC429_003742"
     tRNA            1052..1140
                     /locus_tag="PC429_003742"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1086..1088,aa:Ser,seq:tga)
     gene            1158..1248
                     /locus_tag="PC429_003743"
     tRNA            1158..1248
                     /locus_tag="PC429_003743"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1192..1194,aa:Ser,seq:gct)
     gene            1257..1333
                     /locus_tag="PC429_003744"
     tRNA            1257..1333
                     /locus_tag="PC429_003744"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1291..1293,aa:Pro,seq:tgg)
     gene            1419..1494
                     /locus_tag="PC429_003745"
     tRNA            1419..1494
                     /locus_tag="PC429_003745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1452..1454,aa:Trp,seq:cca)
     gene            1555..1631
                     /locus_tag="PC429_003746"
     tRNA            1555..1631
                     /locus_tag="PC429_003746"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Pro,seq:tgg)
     gene            1638..1714
                     /locus_tag="PC429_003747"
     tRNA            1638..1714
                     /locus_tag="PC429_003747"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1672..1674,aa:Ile,seq:gat)
     gene            1718..1793
                     /locus_tag="PC429_003748"
     tRNA            1718..1793
                     /locus_tag="PC429_003748"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Phe,seq:gaa)
     gene            1801..1877
                     /locus_tag="PC429_003749"
     tRNA            1801..1877
                     /locus_tag="PC429_003749"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1835..1837,aa:Met,seq:cat)
BASE COUNT          517 a          452 c          531 g          486 t
ORIGIN      
        1 taactctaaa aaaaaataaa aaaagtattg acaaaaatag taaaaaatgt taatattata
       61 cttgtcctag caacaagaaa aaatgtggtt ataatagcgg aggagataca cctgtttcca
      121 ttccgaacac agaagttaag ttctctagcg ctgatggtac ttggtgggaa actgcctggg
      181 agagtaggac atagccacgt gaatgttcca aggtagctca acggtggagc aaccggctgt
      241 taaccggtag gctgtgggtt cgagccccac ccttggagcc attatggctc gttggtcaag
      301 aggttaagac accgcccttt cacggcggta acaggggttc aattccccta cgagtcacca
      361 tttgtgggat catagctcag ctgggagagc acctgcctta caagcagggg gtcacaggtt
      421 cgagccctgt tggtcccacc atttgcggcc tggtagttca gttggttaga atgccagcct
      481 gtcacgctgg aggtcgaggg ttcgagcccc ttccaggtcg ccaaaataaa aatgctggtg
      541 tggctcaacg gtagagcagc tgacttgtaa tcagcaggtt gtaggttcga ttcctatcac
      601 cagctccatg taaaactaat acggaggatt tcccgagcgg ccaaaggggg cagactgtaa
      661 atctgttgtc atcgacttcg gtggttcgaa tccaccatcc tccaccaatt aaatatgcgg
      721 gtgtagctca atggtagagt tctggccttc caagccagct gtgagggttc gatccccttc
      781 acccgctcca aagaaagcgt gggtgcatag ctcagctgga tagagcaacg cccttctaag
      841 gcgtgtgtcc ggggttcgaa tccctgtgcg ctcaccacat atatattggg gattagccaa
      901 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      961 caaataaaaa tatggtctat tagctcagtc ggtagagcac ctgactttta atcagggtgt
     1021 cccgcgttcg agtcgcggat agatcaccaa tggagaggtg tccgagtggt ttaaggagct
     1081 ggtcttgaaa accagtgact tcgaaagggg ccgtgggttc gaatcccacc ctctccgcca
     1141 aaaaaatgac tgaatttgga gaagtactca agtggctcaa gaggatcccc tgctaaggga
     1201 ttaggctggg taaccggtgc gagggttcga atccctcctt ctccgccatt caaaaacgag
     1261 gtgtagcgca gtttggtagc gcacatggtt tgggaccatg gggccggggg ttcgagtccc
     1321 ttcacctcga ccaacttaag cttttagttc agttggttag agtgcccaac tcatggctag
     1381 taattctagg gtttgagtcc atgaaggtta ttcttcatag gggtgtagct cagttggtag
     1441 agcgtcggtc tccaaaaccg tgcgccgggg gttcgagtcc ctccacccct gccaaataaa
     1501 aaggacttga aaaaagttgg attttagagt agtataagat agtaaataag atttcgaggt
     1561 gtagcgcagt ttggtagcgc acatggtttg ggaccatggg gccgggggtt cgagtccctt
     1621 cacctcgacc aagaaaaggg cctatagctc aggtggttag agcgcacgcc tgataagcgt
     1681 gaggtcgctg gttcgagtcc agctaggccc accatttgcc cagatagctc agtcggtaga
     1741 gcaggggact gaaaatcccc gtgtcggtgg ttcgattccg cctctgggca ccaggaatac
     1801 ggcggtatag cttagttggc tagagcgttc ggttcatacc cgaaaggtca taggttcgac
     1861 tcctattacc gctaccatct aactatatca ataaagaact ttgaaaatta aacagtaggt
     1921 taatttataa acaagaaaca aaccataaag ccagatattt tgataacaat agtatctgag
     1981 cctgat
//
LOCUS       DAMKMI010000263         1942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMKMI010000263 DAMKMI010000000
VERSION     DAMKMI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.269"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            74..448
                     /locus_tag="PC429_003750"
     CDS             74..448
                     /locus_tag="PC429_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF3849179.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            445..1308
                     /locus_tag="PC429_003751"
     CDS             445..1308
                     /locus_tag="PC429_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3849180.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            1308..>1942
                     /locus_tag="PC429_003752"
     CDS             1308..>1942
                     /locus_tag="PC429_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF3849181.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT          742 a          204 c          300 g          696 t
ORIGIN      
        1 aatcagttga catattatat atatatttat ataatgtgta tatactatat atacaattta
       61 aggaggtgtc ttattgaata tagttattag taattcgagt gggaagccaa tatatgaaca
      121 gattactaca caaattaaaa gtatgattat aagtggagag ttaccagaag gctcagctct
      181 tccaagtatg agactattgg caaaagaact tagaataagt gtaattacta caaaacgagc
      241 atatagcgat ttagaacgag acggatttat tgagacagtt actggtaaag gcagttttgt
      301 atctagtaaa aacatggatt ttgtcaaaga agaacaattt cgtttaattg aagaatacct
      361 acaaaaagca gttgatacag caaaaagtag tgatattaca tatgacgaat taaaagatat
      421 tttattttta ttatataaag gagaatgata agtatggatg caataactat aaaaaattta
      481 aataaaactt ataaagattt tagcctacaa gatatatctt tttcagtacc aaaagggagt
      541 gtaatgggat ttgtaggtga aaatggtgca ggaaaaacaa ctacattaaa agcaatttta
      601 aatttaatat cttatgatag tggaaatata gaaatctttg gtcttgatag taaaaaaaat
      661 gaaaaagaaa ttaaagagca aattggggtt gtatttgagg gaagcaactt tcatgaaaat
      721 ttaaacactg accatgtttc aaaaatcatg agtaaaatct ataaaaattg gaatgacaca
      781 ttatttaaag actacttaaa aaaattgaga gtaccagaca ataaattaat taaggagttt
      841 tcaaagggga ataagatgaa acttagtatt gctgtagcct tatctcataa accaaaatta
      901 ttaatactag atgaggcaac tagttcactt gaccctatcg ttcgtgaaga aatactagat
      961 atatttttag attttataca agatgaagaa cactctatta tcttatcttc acatattaca
     1021 agtgatttag ataagatagc agactatatt acatttatac ataaaggaaa aattgtattt
     1081 agtgaaaata aggatgaatt aattgatact atgggagttt taaaatgtaa acctagtgat
     1141 tttgataacc tttcaagaga ggattattca tattatcgta aaagccaatt tggatatgaa
     1201 gtacttctta aagataagca tagatttatc tcaagacatc caaactgtat tgttgataat
     1261 acgtctatag aagaaattat gttattttat gtgagaggtg ataaataatg aaaggtctaa
     1321 ttttaaaaga tttacttaat ttgaaaggaa atataaagtt tatactttta ttcatcatta
     1381 tgtttggctt tatgtcttct ttaggagatg gaaatgttaa taactttata ggtgttatta
     1441 tagttttatg tactacaatg attgtttcaa cattttcata tgatgatttg aataaatggg
     1501 atagttatgt acttacaatg cctattaatc gcaatgatat tgtattaagt aaatatttaa
     1561 ctatgttaat atttagcttt ataggtgttc ttgtttcact tattgttagt gtaactattg
     1621 gttattttaa aaatacactt atattaaatg aaacactact tattaatgca ctaatcttat
     1681 caatttcagt atgttttggt agccttattc taccacttat ttataaattt ggtacagaaa
     1741 gagctcgttt attaatgatt ttatgctttt tagttccaac actagcttta ttagttttta
     1801 aaagtatttt agaaaatatt agttcaccta tttctataga aattatactt aatacactag
     1861 tatattctct tccatttgtt gctattttac tatttgtgat ttcatatttt atatcatcaa
     1921 aaatctatag taaaaaagaa gt
//
LOCUS       DAMKMI010000264         1872 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMKMI010000264 DAMKMI010000000
VERSION     DAMKMI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.270"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            141..335
                     /locus_tag="PC429_003753"
     CDS             141..335
                     /locus_tag="PC429_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF3849182.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(437..946)
                     /gene="vanZ1"
                     /locus_tag="PC429_003754"
     CDS             complement(437..946)
                     /gene="vanZ1"
                     /locus_tag="PC429_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF3849183.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLVFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1266..1655)
                     /locus_tag="PC429_003755"
     CDS             complement(1266..1655)
                     /locus_tag="PC429_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3849184.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEGDIDD"
BASE COUNT          693 a          260 c          189 g          730 t
ORIGIN      
        1 cctataacct ttattttttt attaaaatgc attataataa aaagtaaatc taaatttgat
       61 acagttttaa caatcattat tttcagaaaa gtgatactat tgaatatatt aagaataatt
      121 agagatggag gaactaagat atgagcgtaa ttgtaatgtg tagtcgttgt ggaaaagaaa
      181 tagataaata tagaaataaa tatataactt attatgagaa aaataccaat gcagaaatta
      241 aagtttgtat aaattgtgcg ttagatttat tagaaaaaac aagagaaaat caagaattga
      301 acatacaaga gtttcaagat acaataattc tttaataatt taggaaagca cctgtatagg
      361 tgctttttat gtgtaaataa ttttatatgt aactgtatct tacgttctat tttaagctaa
      421 acatatatac ttatttctat aagtttgcaa aaataactaa tcctaaaaat aatacaacaa
      481 taattgtcac aatcaatgcc aatacattga taatttttat ggtattattc ttaaatattt
      541 tcgataatag cataaaaaca gctattccta tgattcctcc tagtgtgttt cctatcaaat
      601 ccgttatatc acttgcacct agtgcaaata tatattgaag tgtttcaaat accaaactag
      661 ttataaaaat aggtataact ttttttataa agctccattc ttcttttagc atacacacat
      721 atatcccaaa tggtacaaat atagccacat ttaatataat ctctgatata tctactctat
      781 tattaattat aagtgaccca gcaaaaggta ctaagtttat gcttctaaga ttcatggttt
      841 caagggatga aatatcaaat tgcaacttga atagtattat ccatgttaaa ataattatgt
      901 aaactataaa taatcctttt gtaatattat gttttctaga tttcatttat tacctcctta
      961 ctatatcatt gtattatttt attaatacaa tgatatagta aggtaattta tctgtcaact
     1021 atttcttcta aaattatatt ccttttataa tttcaatatc tttatcatat ttacatgatt
     1081 taagctattg tagtatttca tatatcttta aattttaatt ggtattattc tctatatttt
     1141 ttactatcaa acactaagga aataatcact tttctttata taatgttcta ccatcacata
     1201 aaactaacat taaatcaaaa cgctactctc aattgtctca aactaaaaag atactgataa
     1261 ctttattagt catcaatatc tccttctttc aaatttttaa agtattcttc taacttgtcc
     1321 aagctcttac tatctaaaac ttcatcatca tgtaatgctg aaataaaact cttaaatgaa
     1381 ttgttatgca taaaactaaa aaaactcttt gtctcaactt ttaagtaatc tttctctttt
     1441 atagctatag tataatgcgt acatctatct accttatcag tagttaaaaa gcccttatct
     1501 actaaatttt ttaaaaatgt caatgttgtg ctctttttcc aatcgtattt caacttcatg
     1561 tcttctatga tatctcttga ggctaataca tcatctactc cccaaatata cttcattagt
     1621 tttagttctg catgtggtaa tttattaatc ttcattagtt tacacctctt accaatttat
     1681 gtataactaa actttataac aataagtgta tataatcaat tagattggaa taaatataaa
     1741 ttccaattta ttctatataa aaataattaa taaaaaagaa gtactctact ttagagagta
     1801 cttttggatt atatattcaa aaatttatct aacatgatta gagtatgttt tatgctttag
     1861 tgtgataatt tt
//
LOCUS       DAMKMI010000265         1807 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMKMI010000265 DAMKMI010000000
VERSION     DAMKMI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.271"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            193..1005
                     /locus_tag="PC429_003756"
     CDS             193..1005
                     /locus_tag="PC429_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF3849185.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1094..1285)
                     /locus_tag="PC429_003757"
     CDS             complement(1094..1285)
                     /locus_tag="PC429_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849186.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1556..1795
                     /locus_tag="PC429_003758"
     CDS             1556..1795
                     /locus_tag="PC429_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3849187.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYKNIYN"
BASE COUNT          685 a          205 c          243 g          674 t
ORIGIN      
        1 aaattgaaat aatcactttt tcaagaatgt tataattttt ttattgtatt ctttttgcta
       61 tagtctatga ataaatcaat agaaaatctt ctaagttgct tttaaataat gactttatgg
      121 ttcaattgta ctaaaatagt atatgtaggt tacaaataat ctaataaata ttaaagtttg
      181 gaggtaatag atatggcaga ttgtaattca tgtccatcaa agggaaattg taatagtcaa
      241 tcaaattgtt ctattgaaaa taaccctaac aataaatttg gaaaaataat tggtgttatg
      301 agtgggaagg gtggagttgg aaaatcaact gtaactgctt tacttgctaa taagctaaac
      361 aaaatgggat ataaggttgg tatattagac tcagatataa ctggaccaag tattcctaga
      421 cttatgggtg taaaaaatgt aaaagcttat tctgatggct cttatattta ccctgtagaa
      481 aactcaaata acataaaagt aatgtctata aaccttatga tagatgatga aaatgaacct
      541 gttgtttgga gaggaccttt acttggaggg gttgtaaagc aattttatac tgatgtactt
      601 tgggaagaat tagactattt attaatagat atgcctcctg gtactggaga tgttgcctta
      661 acagttatgc aatctattcc tataagtgga atagttatgg tttcagtacc tcaagatttg
      721 gtatctatga ttgtatcaaa agctgttaat atggctaaaa aaatgaacat aaatgtcctt
      781 ggagttattg aaaatatgag ttatatccaa tgtcctgatt gttctaaaaa aatcaagtta
      841 tttgaaggag aatctactga aaagttttta gatgatttag acttagagct tttaggagaa
      901 cttcctatga ctaaagaaat tatagatatt actcataatg gagttacaga aattagtgat
      961 gatttagatt caatattgac taatgttgtt gaaaaaatta aataaataaa aattttataa
     1021 ttttatattt atcaaaaaaa gaggcttgta tttattttat aatcatacaa ataaatataa
     1081 acctctttta tgtttatcta tgttttaaat acacaaaata ttcttctact tcatctccat
     1141 tttttacttt tactaaatat tcattataat gagcattata tggctgtgtc attttgcatt
     1201 ctattacatt ttctatgtct tctaattcta aaagttctac atcctttaaa aattttcttt
     1261 ctatgagttc cattaatctt tccataatat atcctcactt cccctataat taagataaac
     1321 tatatatcct cttaatgcag agtttatctg tttattatat tatatccaat atattacagt
     1381 ttctaacatc attcataaat tattttaaca aatagagatt gattttttaa taaatatgcc
     1441 ttgataaaat gaaacataga ccaactctta gttttatgta aagaaaactt aaagaaaact
     1501 ataaattata ttcacaaaaa ttactcagaa gatataacag taagtaatat atgtaatgaa
     1561 ttacaactta ataaaagtta ttttttcttt gtttaagaaa aaaagtggat atacttttac
     1621 taatttttta aataaagtta gagttgaaaa aagcaaaaag tatatattaa aaaaagactt
     1681 gtctatttta gacgttgctg tactaattgg attcaatagt caaaattact atagtataat
     1741 ttttaaaaag tttaataact ttacaccaat tgaatataaa aatatttata actaaaaaat
     1801 atcattc
//
LOCUS       DAMKMI010000266         1743 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMKMI010000266 DAMKMI010000000
VERSION     DAMKMI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.272"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            173..1501
                     /locus_tag="PC429_003759"
     CDS             173..1501
                     /locus_tag="PC429_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF3849188.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          590 a          201 c          321 g          631 t
ORIGIN      
        1 gtttttataa tttttttata tttaaacata gctaaaaata aaagattacg aaagctaagt
       61 agagtttatt ttattttaat aaataataaa gctatttttc tatatattgg cataatatat
      121 gcttatttaa ctatgaaatt aaaattaaat aggattaatg aggaggaaaa aaatgggaag
      181 taatgaactg aaaaagactc ttggcgtatc agcagcatta tcaactgttg ttggttccgt
      241 tataggagcg ggcgtatttt ttaaaccaca agcagtctat acattaactg gaggagctcc
      301 aggtcttggt atattggcat ggttaatagc tggaatcata actataacag ctggtcttac
      361 agctgcagag gtatcagttg cgataccaaa aactggtgga atgatggtat atataaaaga
      421 aatttatgga gaaaaattag gttttttaac tggatggatg caaattgtat tattttatcc
      481 agggatgatg gcagcattag gtgttatatt tggagaacaa gcatcagcac taattggtag
      541 tccatcactt ttacttccaa tagcaattgg aattatagta atagttgcag ggcttaatat
      601 gttaggttca aaaactggag gagtcataca aactgtttct acaatttgta agttaatacc
      661 acttatttta ataatggttg ttggatttat taaaggtggt ggaaataatc caatacttac
      721 acctatggtt ggtgaaggat taagcctggg tagtgtacta ggtcaagttt taattgctat
      781 attatttgct tttgatggat ggatgaatgt tggtacttta gctggagaaa tgaagaatcc
      841 tggaaaagac cttccaaaag ctataatagg tggattgtca gttgttatgg ctgtttattt
      901 tataataaat ttagcgtatc tttgggtatt accagctaat gaattagcaa attatgcttc
      961 tcctgcatca gcagttgctg aagttatatt tggttcaatg ggtggaaaaa taatatctgt
     1021 aggtatatta atttcagtgt ttggagcatt aaatggattt ttactaactg gttcaagggt
     1081 ggcatatact ttagctacag ataagacttt accaaagtat ggtatatttt ctaaattaaa
     1141 ttctgctcaa gttccagcaa atgcaatagc attagtatct gttattgcat ctatatatgc
     1201 tttgtcaggt caatttaatt tattaactga cttagctgta tttgctactt ggatattcta
     1261 tgtattaact tttatagggg ttatgaaact tagaaagact catccaaata taccaagaga
     1321 gtataaagtt ccattatatc ctattgttcc tataatagct atagcaagtg gtatatttgt
     1381 tgtagttaat caattgtgtt ttgcaggtat gaaaactact atgatatcta taggaggtct
     1441 tgtaataaca gctataggct taccagtgta tgcttatatg actagaggag taaaaagata
     1501 aaccaagtag tttacaaatg cttcataatt aaaataaaag agactgtctc aaatagagat
     1561 aagtctcttt tttattgtta aaatggtaaa gatatgttta attttacaaa aaaagagcgt
     1621 tccatgaact aaaaagttta ttttaaggta ttcattttat ttttagtttt ttgattataa
     1681 aattaaacat ctaaaaaatt atttatttta aaatacttta ttttaaagtg ttttttatca
     1741 aac
//
LOCUS       DAMKMI010000267         1638 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMKMI010000267 DAMKMI010000000
VERSION     DAMKMI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.273"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            219..1559
                     /locus_tag="PC429_003760"
     CDS             219..1559
                     /locus_tag="PC429_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF3849189.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT          641 a          125 c          263 g          609 t
ORIGIN      
        1 cctagttata gataaattta tatagttatt attaaaaaaa gcaataaaaa gtttaattca
       61 atttagcaaa tttagtgata taattaaaaa agaaaagaaa tatggactta tttggaataa
      121 ttttgacaat ctatttacta cacgtgtaga gtcgtatata attaaattat acataaaata
      181 taaaaaaata atttagtaat acgttaggag aaagtattat gataaagtca agcttatttt
      241 ggaatatttt cattgcgata tcctttatga tagaatggtt tctttgtaag aaaatgctag
      301 attatacaag tgagaaaagg atttctaaca aaaaaataaa cttttgtgtg gtaggtatta
      361 ttgtttttgc tgacataata tatttttttg atgtacctcc aaatataaga gtaattatat
      421 ctatgataat tactattgca ttttataaaa tctcatatga tgtggggttt ataaagtctg
      481 tattaatatc cttaatttat tggatgttac taatcgccac agatgctttg agtatatctt
      541 taacagtttg gcttaattct ataaatgaca tgagtagtat ggggacggga gatatataca
      601 gaattgagtc tataatattg ggaaagatac ttttgattgt gtctttgctt ttatatagag
      661 ccttaaagat aaaacttaat atagaaataa ataaaaggac attattttat atagttattc
      721 ctatagtcgc taatatagca tctttttttg taatttttga atacatattt aatccagggt
      781 ctggtagctt aatacataat ttccaattcg ttatgatttc tgttgtattg cttatatcaa
      841 gtatcacttt agttttaatt gctaaaaaat tacagcaata taatacttta gttgtagaaa
      901 ataatatgat taaaaatact ttgaagtatg aaaatgagta ttatgaagat atagaaaaga
      961 aatatttaaa aactagatta ctaagtcatg atatgaaaaa tcacatcttg tgtataaatg
     1021 ctatgattaa aaaaggtatt gatgttactt cttatttaaa taatttgcag gtagaaattc
     1081 agagtaatga cttgttattt aatactggta attttatatt agatgctata ttaagtgaaa
     1141 agaaagaggt ttgtgataag aatgatatag aactaaaaat aggaataaat tttagttgtt
     1201 gtgaagctgt tgaattagtg gatatatgtc atatattttc aaatatttta gacaatgcaa
     1261 ttgaggcatg taataaaatt gatagtggaa atagagttat ttgtgttaaa ggtgatgtta
     1321 tgcaaaattt ttatttaata agagttgaaa atactaagat gaataagatt attactaaaa
     1381 atggagatat tttgacagac aaaaaaggtt ctcttcatgg acttggaata aaaagtgtaa
     1441 aaaactctgt aaataagtat ggtggtaatg ttgtaattga atatgatgat aaaaaatttg
     1501 tagttaaaat tgctattcca cttagaaatg tgaagaaagc agttaatcat gtgatttaaa
     1561 tgtatataaa tttgatgtgt agaaatttaa tatataagaa ttaatgtata aaagtgatgc
     1621 atagaagttt aatatata
//
LOCUS       DAMKMI010000268         1627 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMKMI010000268 DAMKMI010000000
VERSION     DAMKMI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.274"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            281..1552
                     /gene="serS"
                     /locus_tag="PC429_003761"
     CDS             281..1552
                     /gene="serS"
                     /locus_tag="PC429_003761"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF3849190.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKDFNLDEVVKLDDERRKILQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKTEYVHTLNGSGLAIGRS
                     VAVILENYQQEDGSVVVPEVLRAYMGVSVIK"
BASE COUNT          684 a          167 c          295 g          481 t
ORIGIN      
        1 atacaagatt gataaagaaa aagtcaaaat agataattga ttagcaaaat agatataatt
       61 ataatgaagt taaatctagc aatattctcc taaaattaaa tttcatttat ttaattaaat
      121 ataatttgac aatgggcaaa aaagtgagta aaatataaat aaaataaaaa taacaggttc
      181 ggataaagaa aaaggtttct taacctcaag cataaagtat attatctaag ttatttgaga
      241 ttaagagata aatccaatct taggtactga ggaggaaaaa atgcttgata taaaaagaat
      301 aagagaaaat ttagattgta taaaaaaagc aatggaaaga aggggcgaaa aagactttaa
      361 ccttgatgaa gtagttaagt tagatgatga aagaagaaag attcttcaag aagttgaagt
      421 tatgaaaaat gaattaaata cagcatctaa aaatatacca aatcttataa aagaaggtaa
      481 agatgtagaa aatgaaaaga ttaaactaaa agagttatct gataaaatta aggtaataga
      541 ccaaaatctt aaagaagtag aagataagat ggaataccta ttaatgagaa taccaaatgt
      601 accacatcca gaagtaccac aaggtgaaac tgatgaagat aatgttgaag taagaacttg
      661 gggaaagact actacctttg attttgaatc taaggctcat tgggaaattg gaacagaatt
      721 aggtatactt gattttgaaa cagctagtaa aataactggt tcaagattta ctctttacaa
      781 aggattaggt gctagattag agagagcatt attaaatttt tacttagaca cgaatacaaa
      841 agtaaatgga tatacagaag ttattccacc atttatggca aatagaaata gttttttagg
      901 tacaggacag ttacctaagt ttgaagaaga tatgtttaaa atagaaggtc tggactattt
      961 tatgattcca acatcagaag ttcctttaac taatatacat gctaatgaaa tacttaaatt
     1021 tgaacaatta ccaataaatt atacagctta tactccatgt tttaggtcag aagctggttc
     1081 tgctggaaga gatacaagag gtttagtaag acagcatcag tttaacaaag tagaaatggt
     1141 taaaatagtt gcaccagaag aatcttacaa tgagttagaa aagcttacta acaatgcgga
     1201 aactatgcta caattattga atctgcctta tagagtagta aagatatgta caggtgattt
     1261 aggatttacg gcttctttta aatatgatgt agaagtttgg atgccaagtt acaataggta
     1321 tgttgagatt tcatcttgtt caaactgcga agatttccaa gctagaagag caggaataag
     1381 atttaaaaga gataaagatt ctaaaactga atatgtacac acattaaatg gctctgggtt
     1441 agctattgga agaagtgttg cagttatatt agaaaattat caacaagaag atggttctgt
     1501 ggttgttcct gaagttttaa gagcatatat gggagtttct gttattaaat aagagttaaa
     1561 ttatgtattt ttaagcaata caataatata gaggtaataa aaaataatta atataaaagt
     1621 gatactt
//
LOCUS       DAMKMI010000269         1626 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMKMI010000269 DAMKMI010000000
VERSION     DAMKMI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.275"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(97..1488)
                     /locus_tag="PC429_003762"
     CDS             complement(97..1488)
                     /locus_tag="PC429_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF3849191.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
BASE COUNT          581 a          309 c          181 g          555 t
ORIGIN      
        1 tttcataatc aaagaggaaa ttacatttaa ttttatattt aaatgtaact tcctttattt
       61 aaagtaattc atttatttac aataattcgt catgaattat ttttcttttg ttagtaaatc
      121 catagccatt aatcttgtta atacatttct tattcccaat aatattaaac caaatattat
      181 taaaactgtt gctattcctc caaatatctg taaaaaacca agtgtggctg taaaaccagc
      241 aatctttcct gataataaac ttgcaaaaaa acttgttaga taccaggctc ccatagacaa
      301 cgtagcatac ttagctggag ctaatttatt gaacattgca agtcctatag gagataagaa
      361 taattcacct actgttgtaa atatataagt caatactata aacaatatac tagcctttac
      421 cattccatct ggaccattag ctaatgtagc aaataccata actataaacc caacacccat
      481 tattatcatt cctaatccca ttttaactgg aatagagaaa tcacctcttt tggtatgaga
      541 taatttaacc catagggtag ctaatactgg gcataaagcc acacataata tagcatttag
      601 tgatgtaagc catgctactg gcatttcaaa tccaaataga gttctattta cattatccct
      661 tacataaatc ataaaagaac tttgtgtttg atagtatgct gtccagaata agaccacaaa
      721 taaaaataaa ataaccattg ctattatcct atttttctcc aacttagtaa gtggtccata
      781 ctcaattttt tcacctttat tacctttagt cacaggatat tttccgactt catttaataa
      841 ttttggtgca agaagtgtaa atataatcat acctaacatc atgcctatac cacaaattaa
      901 aaatgccatc ttatatccat aagatactat atctccactt gctcctctta ctgcaaatac
      961 attatcagaa agcaatccac atattattgg agctataaat gctcctacat ttactgccat
     1021 atagaataaa ctgtatgctg catcttttct tgacacatca gttttaggat atatatcccc
     1081 aactatattt gagcaacttg atttaaaaaa accatttcct aaaataatta ttgatagacc
     1141 tacccaaacc attgcaatag atgtagatga agcaaatagt acgaaatctc ctattgctat
     1201 taaaagtgca cctatagaaa tacatttttg ttgacctaaa actttatctg ctaaccagcc
     1261 accgactaat ggtgtaagat atataagacc tgtaaataag ccaaacatgg atgttgcttc
     1321 ttgagtagaa agacctaaac caccttgagc tacacttgct gtaaaaaata ataccaataa
     1381 agctgttaaa ccataataac taaatctttc ccacaaaatt gtcatgcaaa taagccaaaa
     1441 actcttcgga tgaccacttt ttttagatac gctttctgta cttgacataa aaaaacgccc
     1501 ccttttgttt tatagttaaa tttaatatta atattgcttt aaccatttta gcacatactt
     1561 ataatatcat cttttctatg tttactcaat agattatcgc ctgtattttc atttttttat
     1621 tattat
//
LOCUS       DAMKMI010000270         1579 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMKMI010000270 DAMKMI010000000
VERSION     DAMKMI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.276"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            51..1289
                     /locus_tag="PC429_003763"
     CDS             51..1289
                     /locus_tag="PC429_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF3849192.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIKFDCDSKDNSFNGDINVETVHCINEYLSDDDVE
                     HNYNNFHNKDTIKDKAISNISTIKKYIDESFDKNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISLLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT          673 a          141 c          269 g          496 t
ORIGIN      
        1 aatacttatc tagttactag caattattag tagaaatggg ggaaatagag atgaataagg
       61 gattttacaa attaaattca gtggtattga actttgatat taagaatgat gttaacaaga
      121 taagatttaa tcattatata atgagaaaag aaaacataca aagacttcaa ggaactctgc
      181 caaacggaca attttatatg acagtgagaa gagctgctac agatttacaa ctttctattt
      241 ctactataag taggcttgta aaagagtttt tggatttagg gataataaga ttagtgtcta
      301 agggtgtaaa aggtaatcgt tctgtatata gttacgtaag tatagagttt aatggaagtt
      361 tatttgatga tagcaaatat atggaacttg actgtgtatg taatgatgaa tttattgata
      421 atagtggcat aaagtttgat tgtgatagca aagataattc ttttaatgga gatataaatg
      481 tagaaactgt gcattgcatt aatgagtatt tgtctgatga tgatgtagag cataattata
      541 acaatttcca taataaagat actataaagg ataaagccat ttctaatatt agcactatca
      601 aaaaatatat tgatgaatct tttgataaaa ataaatcaga aaattctaac ttacaacagg
      661 agtcaataaa tttcaacttt aatgatacat gcaatgaaat agataaaaga acgaataata
      721 gtatagaaaa taatcaaaat aaaaggccta tgagattgag tgacatcagt cttttaagtg
      781 ataaaggtat gacaaataaa aagaattact ttaatcataa tcatggtata caaaaaggga
      841 ctaaatctgt aacaaagaaa aaagaatcct taaataaatt taataaaaaa aatatacagc
      901 aaagcattat aaatatgcta aataaaaaat caggtaagaa ttttaaaatc gattctccaa
      961 taagcataaa acttatcaat gaaaggctaa aggaaggcta tgtcttagaa gatttctaca
     1021 aagttattga ggtaaaagtg gctaagtgga aaaataccat aatggaaatg tatataagac
     1081 cagaaacttt gtttagctat aagtttgaat cctatgtaaa tgaaaacata tctatgaaaa
     1141 gtcaaagtaa tgttcaccca tgtaaagaag gcaatagtaa tgatagcagt aattatatac
     1201 attctgatta tacaacatat gatggcagtg tgcgtgagag aaaaaaatat gagaatgtaa
     1261 gatactggaa tcctatatgt gaaatttaat tagtttaaga atgtatttat gataaataag
     1321 atattaatag agaatagtga gctatagcaa atgatatatc aatagttggt agtaagttat
     1381 attaagcaag atattactat caggtagtga attatattga acgatatatc aataattggt
     1441 agtaaattat attaagtaag atattactat caggtagtga attatattga acgatatatt
     1501 aataattggc agtaaattat attaaataag atattaatat cgagtaatga attatattga
     1561 acgatatatc aataattgg
//
LOCUS       DAMKMI010000271         1569 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMKMI010000271 DAMKMI010000000
VERSION     DAMKMI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.277"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            92..1528
                     /gene="miaB"
                     /locus_tag="PC429_003764"
     CDS             92..1528
                     /gene="miaB"
                     /locus_tag="PC429_003764"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF3849193.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT          670 a          161 c          292 g          446 t
ORIGIN      
        1 ctagaaaaat aataatatct ttaaaagatt tttcttattt gatataatta aggttatgtg
       61 taatataaga aattaactag gaggaataga cgtgagcaaa agaaaacaag taacaatacc
      121 tatagagaaa atacaagaac aagataaata tatagaacaa atacacagac aaaatgaaga
      181 atacttcaac agaactggaa agagaaaact cgtgtttact caaaccttcg ggtgtcaaat
      241 gaatgaacat gattcagaaa aattatgttc aatgttagaa gagatgggat accaaatgtc
      301 aatgatggtt gaagaaagtg atttaataat atataacacc tgtgcagtta gagaaaatgc
      361 agagctaaaa gtgtatggta acttaggaca attaaaacat ctaaaaggaa aaaatccaga
      421 tatgaaaata gctgtatgtg gatgtatgat gcaacaacca catgttgtag aggaattaag
      481 aaaaaaatat aaacatgtag accttatatt tggaacacat aatctatata aatttccgca
      541 attacttaca gaatcaataa attctgacaa aatgttagtg gatgtatggg atgtggatgg
      601 agaagtaata gaaggtctta gaagtaatag aaaatttgaa ttaaaagcat ttgtaaatat
      661 aatgtatgga tgcaataatt tctgcacata ttgtatagtc ccatacacaa gaggaagaga
      721 aagaagtaga actcctgaag atataattaa cgagataaaa gagcttgttg caaatggaac
      781 gaaagaaatc actttacttg gacaaaatgt agattcttat ggtaagacac ttgaaaatcc
      841 agtttcattc tcagaacttt taagaaaagt gaatgatata gaaggaattg aaagggttcg
      901 atttatgaca tctcatccaa aagatatatc tgatgaagta atatacgcaa ttagagattg
      961 tgataaggtg tgtgagtttt tacatctacc aatacaatgt ggaagcagta gccttttgaa
     1021 aaaaatgaat agacattata caaaggaata ttatttagaa ataatagaaa aagctaaaaa
     1081 ggaagttcca ggtattgctt tttctacaga cttaatgatt ggtttcccag gagaaacaga
     1141 agaagattta ttggacactt tagatgtggt tgaaaaagtt agatatgatt cagcttttac
     1201 atttatttat tctaaaagac agggaactcc tgctgctaaa atggaaaatc aaataccaga
     1261 agatataaag catgatagat ttaatagggt gttagaagct gtaaatagga tttctgcaga
     1321 aataaatgat ggatataaag atagaatagt tgaagttcta gttgaaggaa gaagtaaaaa
     1381 taatgaaaat aagtttgctg gtagaactag acaaaataaa ctcgtaaatt tcgaaggtgg
     1441 aaatgatgat ttaattggta aattagttat ggtaaaaatt actgaaccta gaactttttc
     1501 attaaatggg atattagtaa ataattaata ttgtacaaaa taataaaatt atgtaaacta
     1561 atcaaaata
//
LOCUS       DAMKMI010000272         1557 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMKMI010000272 DAMKMI010000000
VERSION     DAMKMI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.278"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            5..1465
                     /locus_tag="PC429_003765"
     CDS             5..1465
                     /locus_tag="PC429_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF3849194.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSA
                     RLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVN
                     TVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTL
                     VNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLV
                     NDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGM
                     YKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYI
                     KIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLG
                     NAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT          640 a          165 c          263 g          489 t
ORIGIN      
        1 tattatgaaa ttctataaaa gaatattaac attgacttta agtatttctt ttgtatttgc
       61 aaatattcaa ttagtagatg caatatcatc tgtagaaaaa atacaaggaa aaaataagta
      121 tgaaatagca ggtaaaatag cagataaaaa tgcatataaa actgcatata aaactgcata
      181 taaaactgct atattaatta atactagtaa ctctattgca gatggattaa gtgcaagtgg
      241 actagcagga gctttaaatg cccctatact attaactgaa aaaaatacta taccaactga
      301 gacatctgca agattaaaaa atgtatcaaa agtttatata attggaggaa catattctat
      361 tagtacatct gtcgaaaatt cattaaaatc taagaaaatg aaagttgtaa gaattaaggg
      421 aaatgacaga ataaaaacaa gttataatgt tgcaaaagaa ataaattcaa taaaaaaagt
      481 caatactgtt atgctaacca atgcatataa aggagaagca gatgctataa gtattgcatc
      541 tgtagcagca agagataaag caccaataat tcttactaat ggacaaagta tacctttttc
      601 aactagtggt ttaaagtctt atgtaatagg aggaacagct tcaatgagta ccactttagt
      661 aaacaatact aaatctacta gactaggggg gagtactcgt tttgaaacta ataaagcaat
      721 aataaataag ttttataagg atgctagaga gttttatata gctggagctt atgaattaac
      781 aaatgcactt gtaggatctt ctttatcaaa acatggtcca atggttttag ttaacgatgg
      841 tagcaataaa agcattttaa aaaatgctaa gaagataact tctataggat atatagattc
      901 aaatatagta caacagtgtt taaatataac aaatggaatt ggagatatta atactggggt
      961 tgtaaaaaat gttaaaccta ctacaaagac tataaaagat ggaatgtata aggttggaaa
     1021 agatatctca gcaggtgaat acttaataac ttcaaactca ggaagttatg cttcttatta
     1081 tgaagttaca agtgatagta caggtaatgc tgatagtata ctttcaaatg atatttttag
     1141 tgggactaga tatatcacct taaagaatgg tcaatatatt aaaattgagg attcaacaat
     1201 gacattagca aagtatgcta aagcccagaa ggccaagaat ggtaagtttg gaaatggtat
     1261 gtataaaatt ggattagaaa ttcctgctgg agaatatatt ataatgtcaa attcttctga
     1321 tgcatattat gaagttagaa atgatagctt aggtaatgca gagggaatag ttactaatga
     1381 tactttttca ggaagaagat atataacggt tgaggaagat caatatttaa tattaaatga
     1441 ttgttattta attgaaaatg aatagatgta attgtattta atagtaaatc atatatttta
     1501 tttaaggaaa ggtgttttaa gttttaattt aaaacatctt tttattatta taataat
//
LOCUS       DAMKMI010000273         1539 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMKMI010000273 DAMKMI010000000
VERSION     DAMKMI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.279"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            14..469
                     /locus_tag="PC429_003766"
     CDS             14..469
                     /locus_tag="PC429_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3849195.1"
                     /translation="MRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQFIEYNS
                     IIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLMTTGIQY
                     CICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(838..1395)
                     /locus_tag="PC429_003767"
     CDS             complement(838..1395)
                     /locus_tag="PC429_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3849196.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
BASE COUNT          560 a          212 c          161 g          606 t
ORIGIN      
        1 caaagaatac ttaataagag aattaaaaga agatgatatt aaaaatgcat tagacttaat
       61 ctggaacgta tttgaagaat ttgaatctcc agattattgt gatgaaggaa ttacgacatt
      121 tcatcaattt atagaatata actctattat tgaaaaaatc aaagaaaaaa ctctcttctt
      181 ttggggttgt tttgactcta aaaaacttat tggagtctta gctattagaa atttaaatca
      241 tatatgtatg ttatttgtag ataaaaacta tcataaacat ggtgttgcaa aaaaattaat
      301 gactacaggc atacaatact gtatttgtaa taaatttaat ttaactgaaa taactgtaaa
      361 ttcatcacct tatgctgttg aagtttatca taaattagga tttaaagata ctgctgttga
      421 gcaaattgtt gacggtatac gttttacacc tatgaaattg ttaatataat aaattaaata
      481 aaaagcaaag tctcttaaat attttatagt tactttgctt ttaaaccaag atttaaaata
      541 atactatctt ttttgttttt taataatata ctttaaaatc cttactaata tgaactataa
      601 tttatttttt gatatccttt ttgattaaca tataaattaa ttcaattatt aaacataaac
      661 tatgttgttt ctctttatat ctcttctttg taaacaatat tgggcatttg aaaataaata
      721 ttcttaaaat atactgttct cctgttgatt ttcctttggt acaaatgata aaaatttaat
      781 ttacatgccc cataatgaag tgttcaataa ataagttaaa ataatttatt tacctcctta
      841 atcatatttt ttatgaatac ctaataacac agtatcaata gaatcgacta aaccttttat
      901 agcttcttct ttactcaaaa atgatttccc aagttgcaaa gtcgaataac catgaagtac
      961 actcattatt aagtttaata cttcacctgt attctttgta tttagattac aagaaagaat
     1021 aagtttttct aataaggatt tatattcatc aaagatttct aatgtttcat tatttccatg
     1081 ccaagtagcc cactgaattg tttcataaac accaggatta gaaattataa aattaaagta
     1141 tgctatactt atacacttta tagctgtatc tccaaaattt ccaatagccg cctttatcat
     1201 ttgactattc atctctctca ttcctttatg agctacttca agtaataaat catctagact
     1261 ttctatatga ttgtacaaag atggtgtacg tattcctagc ttttctgcaa taacttttaa
     1321 agaaactttg ttaagcccat ctttattaac tatatctgat gtagcttgta taactatttc
     1381 tctactaaca cgcatgattt tccctctttc atttaaaatt aattacttta ttagtttaac
     1441 taatctaatt agtttcgtca attattgtaa tttataaaat tctttatact attataaaat
     1501 cttgacagtt taattataat ttaatataat actaattaa
//
LOCUS       DAMKMI010000274         1490 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMKMI010000274 DAMKMI010000000
VERSION     DAMKMI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.280"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(283..594)
                     /locus_tag="PC429_003768"
     CDS             complement(283..594)
                     /locus_tag="PC429_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849197.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(650..1243)
                     /locus_tag="PC429_003769"
     CDS             complement(650..1243)
                     /locus_tag="PC429_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3849198.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT          543 a          200 c          194 g          553 t
ORIGIN      
        1 ggaagtatag aaagctaatc taaagaagta tagtaaacta atttaggaag tatagaaaat
       61 taatctaaag aagtataata aactaattta agaaatatag aaaattaatt taaagaaata
      121 caatacatta atttttaagt aacattaaat taatgtaagt aaaataatgt tcatatgaag
      181 atttaaaggt gcattttctg aagctaagta tatgttgtag agtgttaggt ctctctatcc
      241 tgtgaagttt aatagctcaa gaaaatgcac tatttatgat gattattttg ttttatttcc
      301 aattttggaa ataactattc taattaaatc taaaattata ccataaataa gaaaagaaag
      361 tatagatgct atgtaccaat aaatcgaaat tgaccctgta attgttctac atgggatttg
      421 catacctgaa tccataaaaa caaaaaaact atcaactctg gaaataatag acaaaatagg
      481 atttaaacct attagaacaa tactttttga gtcttgacca atttgatgca tataaatctc
      541 aaataatgca attaaaaaaa accaaaatgt aaaagattta ataatttttt tcatcaaaat
      601 acctcacata atataaattc tgtttgcact atttgcttac tacaactatt tatctccatt
      661 cagtcacatc acttaattgt aatctaggtc ttcttgatgg ttcttcatca ggataaccca
      721 aagatattgc tgccactaat tgactatctg tatttaacca ttcacataat tcacgatatg
      781 caaagtatac atcacaaatc caaagactac ctagacctaa ttccacagct gtaagcgaca
      841 tattctgaat tgcagctcca attgattgca tgtttgccat ttcatagaat cgttcttcag
      901 gggatagctt ttctaaaggt gatttaccta atatatttaa aataaaaatt gttactggag
      961 cttgtttcat gatttcaact gtgtaatttg caccagcgat atgttgacga cttcctggaa
     1021 gaagcccatt atcattaatt tcattttgaa tacctttgct catggcttta agcatagatt
     1081 ctttttcctt ttcagtgatg acaacaaaac gccatggttg cctgttttta gatgaaggtg
     1141 catttattcc agcttcaatt attttctcta tagtttcatg tgaaatgctt tgatttttat
     1201 attttctgat acttcgtctt tttgatatag aatcacttat cattttaagt aacctccgtt
     1261 tattttttgg aaaaatctat ttaattttta ttttctattt taattatttt agcattaatc
     1321 agaaaaaaag tcattgattt tagtatacaa ttattaatga ttatattaag aattgtatca
     1381 aaaagtatgt gattatacta agggattata ttaaacatta tatttaaaat tgtatgaaag
     1441 gttgtattta atattatatt gagagatata tatattagga tttatatata
//
LOCUS       DAMKMI010000275         1477 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMKMI010000275 DAMKMI010000000
VERSION     DAMKMI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.281"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            1..612
                     /locus_tag="PC429_003770"
     CDS             1..612
                     /locus_tag="PC429_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF3849199.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            910..1248
                     /locus_tag="PC429_003771"
     CDS             910..1248
                     /locus_tag="PC429_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF3849200.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT          584 a          167 c          195 g          531 t
ORIGIN      
        1 atgataaacg atattaaaaa taaattcaaa aattacaaac catatataaa tggatacaag
       61 aatatgaaga gagcatctgt tttaattcca atagtagaaa taaataacac acactatata
      121 ttatttgaag ttcgctcaaa aaatttgaag catcaaccta gtgaaatttc ttttccaggt
      181 ggtaaaatag aatcaggtga gactccatat gaagctgtta ttagagaaac ttgtgaggaa
      241 cttggaactt ttagtgacaa tatagaagta atatcaagtc ttgacctcct tataacacca
      301 gtaaagttta tcatacatcc atacttagga tatataaaaa atattaataa tttaaatata
      361 aacaaggatg aagttgacca tacttttttg gttcctataa aatatctttt agagaatcct
      421 ccaaatactt atagtaatac tgtaaaagtt tttccagacg aaaatctacc atatggttca
      481 attccaaacc aaaaagatta taaatttgaa agtggaagtt atcctattct gttttataaa
      541 tataaagatt atgtaatttg gggaatgaca gcaaaaatac tagaaaactt tttaaatttt
      601 ttatcagaat aacactcata ataaatcatc aaaactttta cataagaaat ttataaaagt
      661 ttctaaattt aacaattcta aaatatcaat aactatattt tgtagcactt ttttatttta
      721 ttattgtttg aatatttgat ggttttcaat gcaatttcaa cataaatact atgtattagt
      781 gtatactgtt gatgaattaa agtatgataa tctatttaat aaagtatgaa gtaaatgatt
      841 ttaatgtgcc tttatggtaa aattactaat aagtaataaa attaattaaa atatatggag
      901 gtgcttataa tgtttgctgt aggatttgtt gttataattg cacttgcctt tttcatttta
      961 agtgtactat tatttaatgg aaaagccagt atgctactag caggttataa ctctatgagt
     1021 aagaaagaaa aagcacgata cgataaaaaa aaattatgta gaagttctag cattgttact
     1081 tttattgtct ctataatgct atttattatg gcgtttttgg gatatagaat agagagtggt
     1141 caaatggacg aaaaaaaaat ggttcccttt tctataatat ttataatagt catactttcc
     1201 agtgtatttt ttaatatcct ctactctaac aaaaaatgta aaaaataaat agttgtaagt
     1261 tgagcagtta attataaaag attgactgct ttttcatgta tattgactac tcttattaaa
     1321 tattttaaca tttaaaaatc tatgtaaaaa taatgaatat agtacaaact tctaataatg
     1381 attttacctt tgtggttaca gtccactata tataatttaa tatgataaca gggaattact
     1441 aatgacaact aggtaagctt ttataaaata taataaa
//
LOCUS       DAMKMI010000276         1434 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMKMI010000276 DAMKMI010000000
VERSION     DAMKMI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.282"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            40..1404
                     /locus_tag="PC429_003772"
     CDS             40..1404
                     /locus_tag="PC429_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF3849201.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLVAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
BASE COUNT          573 a          158 c          242 g          461 t
ORIGIN      
        1 aatgtattaa tataattctt aaagaaagga tttttaataa tggatataaa agagttagtt
       61 aaaatgcaaa gaaaatatta taatacaggt aaaacaagag atatatcgtt tcgtatagaa
      121 cagttgaaga aacttaagtt agttgtatca caaaatgaag aaaaaatact tttagcacta
      181 aagaaagatt taaataaatc ggattttgaa ggttttatga cagaagtagg catgttttat
      241 agtgaattaa actttgctat aaagaatata agaaaatgga gcaaaataaa aagagttaaa
      301 tcaagtatgg ttaattttcc atctatatcc aaagttgttc cacaacctta tggtgttact
      361 ctcataatgt caccttggaa ttatccattt caattagcac ttataccatt agtttggtca
      421 ttagtagcag ggaattgtgt tatattaaaa ccttctgaat attctgtgtc aacatctagt
      481 gtagttaaag acatagtgga agatactttt tcaaaagaat atgttgcagt tgttcaagga
      541 agtcaagaag aaagtgaaaa gttactattg gaaagatttg attatatatt ttttacagga
      601 agtactaatg ttggtaaaat aatcatgaaa tcagcatcag agcatttaac tcctattact
      661 ttagaacttg gaggaaaaag tccttgtatc attttaaaag atgctaatat agaccttact
      721 gccaaaagac ttacttgggg aaaactaata aatgcaggac aaacctgcgt ggctccagat
      781 tatgttttag tgcatgaaga tagaaaaaat gagttaatag agaaaataaa atattatact
      841 aataagtatt ttggagataa tccatgtaat aatgagcaat ttcctaaaat cataaatcag
      901 agacatttta atcggatatt atctcttatt gataaagata agatagtcta tggtggaaat
      961 tataataagg agacattaaa gatagagcca actatagtag acaatgttaa ttgggatgat
     1021 aatataatga aggaagaaat atttgggcct atatttccca ttttaacata taaagattta
     1081 gatgaagtaa tacaaaaaat tatacaaatg ccaaatccat tggcacttta tatttttaca
     1141 aaaaataagt acctagaaaa taaactttta gaaatgattc cagcaggcgg atgttgtatc
     1201 aatgatacag ttactcatat agctacgaat tacttacctt ttggtggtat tggtgaaagt
     1261 ggtatgggct catatcatgg aaaagctgga ttcgatacat ttactcacta taagagtgtg
     1321 ttaaaaaaat taaatttgga tgtccctatt agatatgctc catacgataa taaattaatt
     1381 aaggttctta aaaagataat gtaggttata ttgtagaata ataaatataa ataa
//
LOCUS       DAMKMI010000277         1409 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMKMI010000277 DAMKMI010000000
VERSION     DAMKMI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.283"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            93..1310
                     /gene="nth"
                     /locus_tag="PC429_003773"
     CDS             93..1310
                     /gene="nth"
                     /locus_tag="PC429_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF3849202.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINK
                     KGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKG
                     ETEKKTKKSSSNKATQKTSKETKSKFKIKNESANEKDVNKILDELEKLYPDAKCELNY
                     GTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKS
                     KSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVF
                     RVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIK
                     EDCNYYKELNETK"
BASE COUNT          667 a          103 c          274 g          365 t
ORIGIN      
        1 tgttttatat attgatgttt atttttttgt tgatatgatt aaactattta taaatagcgt
       61 tttataaatt ggtattttga agggggataa ttatgaaaaa tgtaaatcta aaagagataa
      121 atgaaaaaga tgtaattttg gaagaagaga taaataaaaa aggaatggat aagaaaaata
      181 taattgagaa ggaagagata aataaaaaag gaatagataa gaaagatata attgagaagg
      241 aagagataaa taaaaaagga atagataaga aagatataat tgagaagaaa gagataaata
      301 aaaaaggaat agataagaaa gatataattg agaagaaaga gataaataaa aaagaaatgg
      361 ataagaaaga tataattgag aaggaagaga taaataaaaa aggaatgaat aagaaagata
      421 taattgagaa ggaagagata aatgaaaaag aaatagataa aatgattggt aaaaaagatg
      481 agataattga tgagaaagat atagagaatt ttatggatat tgaagaagat aaaaaaagta
      541 tcaaagtaag taaaaaaagt actaaaaaag gtgaaacgga gaaaaaaaca aagaagagta
      601 gttctaataa ggctacacaa aaaacaagta aagaaactaa aagtaagttt aaaattaaaa
      661 atgagtcagc aaatgaaaaa gacgtaaata agattttaga cgagttagaa aaactgtacc
      721 cagatgctaa atgtgagtta aattatggga cagcctttga acttttaata gcaactattt
      781 tatcagcaca atgtactgat gtaagagtga ataaagttac ttcagagttg tttaaaaagt
      841 acaacacagc tagagacttt gctaatttga gcattgaaga aattagtaaa gagataaaga
      901 gttgtggtct ttacaaaagc aagtctcaaa aaataaagga tacatctgaa cagctatgtg
      961 aattatatga tggtgaagta cctgatagct tagaaaagct tataaaatta cctggtgttg
     1021 gaagaaaaac ggcaggggtt gtacttagta acgcttttaa tcatccagca atagctgtag
     1081 atactcatgt atttagagtt agcaatagaa taggaatagt ggacgagcca aatcctcaaa
     1141 aaacagaatt tgcacttatg gaagctatac caaaagaaag atggtcacac tcacatcatg
     1201 tattaatttt tcatggaaga agaatgtgta aagctagaaa tccagaatgt gcaagttgcc
     1261 caataaaaga agattgtaat tattataaag aacttaatga aacgaaataa cttttagaaa
     1321 gtaagttgta caagagggat tttctgatat aataatactt gtgaaaatgt tagataaaaa
     1381 actattatat acatgaaata aaaattatt
//
LOCUS       DAMKMI010000278         1349 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMKMI010000278 DAMKMI010000000
VERSION     DAMKMI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.284"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>1349
                     /locus_tag="PC429_003774"
     rRNA            <1..>1349
                     /locus_tag="PC429_003774"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          357 a          292 c          408 g          292 t
ORIGIN      
        1 caaggtttcc aggggaaggt tcgtccgccc tgggttagtc gggacctaag ctgaggccga
       61 aaggcgtagg tgatggacaa caggttgata ttcctgtact accgataacc gtttgagaga
      121 agggatgaca cagtaggata agctaagcac actgttggtt atgtgtgccc aagcattgag
      181 gcagtcaaag taggcaaatc cgctttgata atgctgggat gtgatgggga gcgaaattta
      241 gtagcgaagt agctgatttc acactgtcaa gaaaagtctc tatcgaggtt aaaggtaccc
      301 gtaccgcaaa ccgacacagg tgggtgagga gagtatccta aggccagcga gagaactgtt
      361 gttaaggaac tcggcaaaat gaccccgtaa cttagggata aggggtgcca ccatcaggtg
      421 gccgcagaga ataggcccaa gcgactgttt accaaaaaca taggtttctg ctaagtcgca
      481 agacgatgta taggagctga cgcctgcccg gtgctggaag gttaagggga tctgttagag
      541 caatcgaagc agtgaactta agccccagta aacggcggcc gtaactataa cggtcctaag
      601 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaac gatttgggca
      661 ctgtctcaac aacagactcg gtgaaattgt aattccggtg aagatgccgg atacctgcga
      721 caggacggaa agaccccatg gagctttact gtagcttgac attgggtctt ggtactacat
      781 gtacaggata ggtgggaggc tttgaaacca ggacgccagt tttggcggag ccatccttgg
      841 gataccaccc ttgtagtact gggactctaa ccataggcca tgaatctggt cttgggacac
      901 tgtcaggtgg gcagtttgac tggggcggtc gcctcccaaa aggtaacgga ggcgctcaaa
      961 ggttctctca gtacggtcgg aaatcgtacg tagagtgtaa aggcaaaaga gagcttgatt
     1021 gcaagacata caggtcgagc aaggatgaaa atcggactta gtgatccggt ggttctgcgt
     1081 ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt atctccccca
     1141 agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcatcaca tcctggggct
     1201 gtagtaggtc ccaagggttg ggctgttcgc ccattaaagt ggtacgcgag ctgggttcag
     1261 aacgtcgtga gacagttcgg tccctatccg tcgcaggcgt aggaaatttg agaagacctg
     1321 tccttagtac gagaggaccg ggatggacg
//
LOCUS       DAMKMI010000279         1338 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMKMI010000279 DAMKMI010000000
VERSION     DAMKMI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.285"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="PC429_003775"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="PC429_003775"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="PC429_003776"
     tRNA            179..253
                     /locus_tag="PC429_003776"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="PC429_003777"
     tRNA            259..344
                     /locus_tag="PC429_003777"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="PC429_003778"
     tRNA            360..435
                     /locus_tag="PC429_003778"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="PC429_003779"
     tRNA            443..517
                     /locus_tag="PC429_003779"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="PC429_003780"
     tRNA            532..605
                     /locus_tag="PC429_003780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="PC429_003781"
     tRNA            611..686
                     /locus_tag="PC429_003781"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="PC429_003782"
     tRNA            692..768
                     /locus_tag="PC429_003782"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="PC429_003783"
     tRNA            779..853
                     /locus_tag="PC429_003783"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="PC429_003784"
     tRNA            863..936
                     /locus_tag="PC429_003784"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
BASE COUNT          401 a          265 c          302 g          370 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc atttcgaaca cagaagttaa gctccctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacacc gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataataa atataactaa ttagtaagag ttatatacat tatattgtat
     1201 actttatggt atttatatat tgaaataact tatgaaatta tttataaaga gattcatgag
     1261 catatttttg tctaaacaat aagtataaga aattataaaa agttagagac tatagtgaat
     1321 ttacaattta atgtaata
//
LOCUS       DAMKMI010000280         1337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMKMI010000280 DAMKMI010000000
VERSION     DAMKMI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.286"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            27..101
                     /locus_tag="PC429_003785"
     tRNA            27..101
                     /locus_tag="PC429_003785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC429_003786"
     tRNA            116..200
                     /locus_tag="PC429_003786"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC429_003787"
     tRNA            210..293
                     /locus_tag="PC429_003787"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="PC429_003788"
     tRNA            323..399
                     /locus_tag="PC429_003788"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="PC429_003789"
     tRNA            407..482
                     /locus_tag="PC429_003789"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="PC429_003790"
     tRNA            571..659
                     /locus_tag="PC429_003790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="PC429_003791"
     tRNA            663..738
                     /locus_tag="PC429_003791"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="PC429_003792"
     tRNA            745..818
                     /locus_tag="PC429_003792"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="PC429_003793"
     tRNA            833..909
                     /locus_tag="PC429_003793"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="PC429_003794"
     tRNA            933..1009
                     /locus_tag="PC429_003794"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="PC429_003795"
     tRNA            1017..1093
                     /locus_tag="PC429_003795"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="PC429_003796"
     tRNA            1102..1177
                     /locus_tag="PC429_003796"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="PC429_003797"
     tRNA            1185..1258
                     /locus_tag="PC429_003797"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="PC429_003798"
     tRNA            1265..1336
                     /locus_tag="PC429_003798"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          320 a          309 c          355 g          353 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatatatatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAMKMI010000281         1328 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMKMI010000281 DAMKMI010000000
VERSION     DAMKMI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.287"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..324
                     /locus_tag="PC429_003799"
     CDS             <1..324
                     /locus_tag="PC429_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3849203.1"
                     /translation="ICIEDLQVKNMIKNHKLSRSISDVSWSEFIRQLEYKANWHGRQI
                     VKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLI
                     TIQNK"
     gene            500..1255
                     /locus_tag="PC429_003800"
     CDS             500..1255
                     /locus_tag="PC429_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HDF3849204.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          553 a          108 c          242 g          425 t
ORIGIN      
        1 atttgcatag aagatttaca agtaaagaat atgattaaaa atcataaact atctcgctca
       61 atttctgatg tgtcatggtc agaatttatt cgtcaattag aatacaaggc taattggcat
      121 ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt cacaaatatg caataaatgt
      181 gggtataaaa acgaggaagt gaagaactta aatataagag aatggatttg tcctagttgt
      241 aatgaaactc acgatagaga tataaatgca agtataaata tattaaaaga aggattaaga
      301 ctaataacaa ttcaaaataa ataacgtatg taagaaccgt aggaactacg gggatagcct
      361 gtggagaatt agtaagacat attttagtat gcaaaattct gtgaagcagg aaccctgtga
      421 ctttagtcat gggaggttca gaattacagt ttatatatta taataaaata aattgttaat
      481 tttaaaagag gtgaatttta tggcacatat atgctttgag gatatttgtt atttgttatt
      541 aaaagaagca atgaagttaa agttaatacc acataataaa attgtattat ttgaagatga
      601 tttatcaata ggtaatattt ttgatgtaaa taactacgaa ttaagagaaa atatttttaa
      661 gattagaaat gtttttgaaa ataatccgaa actattattc aagccagatt tgttagaaga
      721 taggaaatta tcaaaatgta gaaatataat aatatggtgt gcaaataacc caaaagattt
      781 gtgtggagta tattatgtag tgagtaagtt tcaagataaa aatataaaga ttgcattagt
      841 agatgaaaaa aagacagaag atgctctaat tagatattca tttaccagtg aaatgtctcc
      901 agaagatttt ccattctttt tagataagac actagaaatt ggctttaatg aaaaactaga
      961 atttaaaaat aaatgggatg tgttagttaa agaaaattct atttttagag cattagtagg
     1021 taacgaaatt aaaagtgttg aagaaactta ttttgataaa tatatattaa gaaatatcag
     1081 tgacaaatgg caacctttaa ttgaagtggt tgcaaactgt atgtttgaag atgaaatgcg
     1141 tgtaaaagat tggtttatac tttggagatt agagaagatg gcaagtttaa aaatagtaaa
     1201 aataaaagga agtaaagggg atttttattc tgataaggag ataagaaaag tttaaaaatt
     1261 gaatttataa taaattgtat taaataatgg gtataactat gaagaaaaat atcttaacag
     1321 tattaata
//
LOCUS       DAMKMI010000282         1306 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.288, whole genome shotgun sequence.
ACCESSION   DAMKMI010000282 DAMKMI010000000
VERSION     DAMKMI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.288"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            24..1304
                     /locus_tag="PC429_003801"
     CDS             24..1304
                     /locus_tag="PC429_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF3849205.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
BASE COUNT          557 a          170 c          159 g          420 t
ORIGIN      
        1 attaaagata aaaggagtat tttatgaaca tacttggatt aatagttgaa tataacccat
       61 ttcataatgg acacatatat catttatcta aatctataga aaaaacaaat gcaacacata
      121 ctgtagcaat aatgagtgga aattttctac aaagaggcga accagcttta tttgataaat
      181 acactagagc aaaaattgct gtcgaaaatg gtgtagactt agttatagaa ttaccaactt
      241 tatttgcttg tcaaagtgct gaaatttttt ctcatggtgc tataacaatt ttaaattcac
      301 taaactgcat aaattctata tgttttggta gtgaagaagg aaatacagaa attctttata
      361 atatagctga aattttagca aatgaaccaa atgaatttaa gttactttta aaaaaatatt
      421 tagatgatgg tctgttattt ccaacagcta gagctaatgc actttttgat tatatacatc
      481 aagacaaaaa aaataatttt atacaaaatt attcaaagga acaattatca aaaacattaa
      541 attcatcaaa caacatactg ggtatagaat acataaaaaa cctcatccat ttaaacagta
      601 gtataaaacc atttactatc tccagggtcc aatcagagta taattccgaa gaaattgaga
      661 gtgatatttg ttctgctact gctataagaa cttcacttaa agaaaataca gacctttttc
      721 atttaaaaaa tgttattcca gaaaaaactt ataacatcct aaatgaaaaa atagataatg
      781 gttttttacc tatgtttgat gacttcttct ttgatatttt aaaaacaatt atacttagag
      841 atatggacac tttaaaagag tattttgaag taaatgaagg catagaaaac agaatctata
      901 aagatatctt tacatctgaa tttctacatg aactacaatt agcaattaaa tccaagcgat
      961 acactttaac caaaatcaaa cgaactctaa acaatatatt gttaggtatt aaaaaaagcg
     1021 acatgcaact tataaaaaac atagatacta taccttatat aagaatactt gcttttaatg
     1081 ataaaggtag agaaattcta aaagagataa aaaataaatc tgaaataaaa ataataaaca
     1141 agttttcaaa tatatcattt tcattagatg atacgatttt taaaacactc ataagttatg
     1201 atataaaagc tacaaatata tataattcaa tatattataa aaacaataaa gatttagtaa
     1261 aaggccctat ggatttttat actcatccaa tatatgtaaa gtagtt
//
LOCUS       DAMKMI010000283         1247 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.289, whole genome shotgun sequence.
ACCESSION   DAMKMI010000283 DAMKMI010000000
VERSION     DAMKMI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.289"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            56..874
                     /locus_tag="PC429_003802"
     CDS             56..874
                     /locus_tag="PC429_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF3849206.1"
                     /translation="MSTSLNTISIFNVKGGVGKTTLTILTAMYLSKLNKKVLIVDGDF
                     QANTTQFIHNTLFEGNTMFEALAYKTKAEDIIIKSPLKDFPSIDLIGSKLECCVLGEL
                     LVTETNREKAVYRWFAKNIGTLGKYDYILIDLSPSYDVVTRNFLLISDSIVTPLEFQD
                     IASIRGCNLFYTKFKEDLEKLEIDNNVKKAIVVNRYTSRKLSTGDEFNNQLEKYDNIK
                     KVLLNTRISEGTIVKNAILNKTDIEEYCKKIKKAHKVREEFKNYIDELFERGIL"
     gene            846..1235
                     /locus_tag="PC429_003803"
     CDS             846..1235
                     /locus_tag="PC429_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849207.1"
                     /translation="MNFSKGEYYKMKFDSEKNENKVDYRDITEKRIQDTFKKNSNSIV
                     TSLLEVKENEEVKKESISIYFESDDINVLKAVAQIKKTTINKLVLDVLKPVIDTTRID
                     LEDSEDIEKLAKSYDKRKKGRGRKIVK"
BASE COUNT          546 a          115 c          208 g          378 t
ORIGIN      
        1 ttcggaatat atgatataat agtattgtat aaaaataaaa aagggggtaa tttaattgag
       61 tacaagtctt aacacaattt ctatttttaa cgtaaagggt ggagtcggta aaactacttt
      121 aacaatttta acagcaatgt atttatctaa attgaataaa aaagttctta tcgtagatgg
      181 agattttcaa gcgaatacta cacagtttat acataacaca ttgttcgaag gaaatacaat
      241 gtttgaagca ttagcttata aaactaaagc ggaagatatt ataataaaat ctccactaaa
      301 agattttcct agcatagatt taataggaag taaacttgaa tgttgcgtgt tgggggaatt
      361 attagtaact gaaacaaata gagaaaaggc agtttacaga tggttcgcta agaacatagg
      421 aacactcgga aaatacgatt atattttaat agatttgtca cctagttatg atgttgttac
      481 acgtaatttt ttattaattt ctgatagtat cgttacccct ttagaatttc aagatatagc
      541 ttctattcga ggatgcaatc ttttttatac taagtttaaa gaagacttag agaaactaga
      601 aatagacaac aatgtaaaaa aagctatagt tgtaaataga tatacaagca gaaaacttag
      661 cacaggagat gagtttaata atcaattgga aaaatatgat aacataaaaa aggttttatt
      721 aaatacacga ataagcgaag gtacaattgt taaaaatgca attttaaata aaacagatat
      781 agaagaatat tgtaagaaaa ttaaaaaagc gcataaagta agagaagaat ttaaaaacta
      841 tatagatgaa cttttcgaaa ggggaatatt ataaaatgaa atttgattca gaaaaaaatg
      901 aaaataaggt tgattataga gatattacag agaaaagaat acaagatact tttaaaaaaa
      961 attctaacag tatagttact tctttacttg aagtaaaaga gaatgaagaa gtgaaaaaag
     1021 aatctatatc tatatatttt gaaagtgatg atataaacgt tttaaaagca gttgcgcaga
     1081 ttaaaaagac tactattaat aagttagtct tagatgtttt aaaacctgtt atagatacaa
     1141 ctagaataga tttagaagat agtgaagata ttgaaaagtt ggcaaagtct tatgataaaa
     1201 gaaaaaaagg aagaggaaga aaaatagtaa aataatttta taataat
//
LOCUS       DAMKMI010000284         1236 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.290, whole genome shotgun sequence.
ACCESSION   DAMKMI010000284 DAMKMI010000000
VERSION     DAMKMI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.290"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(537..1115)
                     /locus_tag="PC429_003804"
     CDS             complement(537..1115)
                     /locus_tag="PC429_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF3849208.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQKSVCL"
BASE COUNT          425 a          201 c          141 g          469 t
ORIGIN      
        1 aaacgaacga aaattatcat actaaaacat aagacatgct ataattgtat tagataaatg
       61 cttgaatata taccaaaagt actcttttta taagagtgct tattttttga aattcattag
      121 catataaact atcaagaacc ttactcaaca cactttaaaa tcgatttaaa tagctctttt
      181 tcatacacaa aattaatgat ataataaaaa aaatagaagt gtattggtag tacacttcta
      241 tagttgcaat taagcattct catgagcggg agtgcttttt tcatttctcc aaacaacatt
      301 atttttttca tcaataatta ttaaatcaat ctcattatct tttaaaatct ctgttgcagt
      361 acaaatgaga ttatctaagt cgaaacttcg agttatctat agttgtttgt tatttaatgc
      421 tcttttaagt atataagttt taaaacacat ttatatcacc ccttaaacac taagacagtc
      481 cctttttata ttaatatatg tatttatgtt aattaaattt tacaacgcct tttacgttat
      541 aaacaaactg atttttgtac atcagttctt acattaacat tgcctgcttt tagattttct
      601 ccaatagtaa gacctttttc ttttagaaca tattcaaaag tcattagtgg aactccacct
      661 tttctaccac ctaatatttc ggttcctttt aaatcattgt aagagaaatt aggcattttt
      721 ttcctagcaa ctagaaaact accatctctt tttgtcaatt gagcaaagtt cactgcataa
      781 tcgtttttgt tttgattata tacatatata gatgcttcag gacccattaa acctacgttt
      841 gcttcacctg aaatcaaagc tgccatagtt ttatctgcac cttgggtatt tataaggtca
      901 atctttatac cttcatcttc aaagaatcct ttcgtaatag cagcatactg tggagcataa
      961 aaaacagagt gagtaacttc tgctatagtt actttatcca gatttttaga cgaagaagat
     1021 tcatcttttt tgtcattaga gttaaaatca catccagtta gacatattgt acttacaatg
     1081 atagagcttg ctattgcgac tattttttta aacataataa atacctccca taagacatac
     1141 gaatagtata tagtattaaa tatttattgt agttgttaca aaaatgtatc tatacttata
     1201 atatttctac aaaaacttaa taataatttt cagttt
//
LOCUS       DAMKMI010000285         1181 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.291, whole genome shotgun sequence.
ACCESSION   DAMKMI010000285 DAMKMI010000000
VERSION     DAMKMI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.291"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            58..789
                     /locus_tag="PC429_003805"
     CDS             58..789
                     /locus_tag="PC429_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849209.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRDKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(946..1152)
                     /locus_tag="PC429_003806"
     CDS             complement(946..1152)
                     /locus_tag="PC429_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849210.1"
                     /translation="MSKYSSLWEYFKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          495 a          126 c          146 g          414 t
ORIGIN      
        1 tcataaacaa atttattttt cacaagtaca atacttattt aaaaaaggag gtttaatatg
       61 ttaagtaaaa aaaatgtaag tttaattatt ttagtaataa ttttaagttt attaactgct
      121 atatttattt tcaataataa aaatactaac aataaattga aagaagttac taagaataat
      181 gaagaaatag tacctaaaaa cttagaaggt ttaagtatag aacaattaat agagattaga
      241 gataaagcca ttgataaaaa tttagaaggt cttagggaat ttggagaaga ttcagtaaaa
      301 aaacgtaaag attcaatttt aaactttgaa tttggattag tagcttttgt taacaagaat
      361 tatagcaact taacagaaaa agagaaatta ataagacaaa ttaatattgt taactatgaa
      421 aactctataa atgatataat ggatccttca ggttcattct ttgaattagc aaatcagaat
      481 taccaagtga atgagtttct attaaaaaac ggaaaagatt ataaagaatg gtcatatgaa
      541 ggtctagatt ttctaattga tcatctagaa gaactgtgca aatttgttga ttttgatgta
      601 aaagatgtaa tagatattat tgatcctaca ataaaaacag acctttcaga ggaacaaaag
      661 aaatctttga atgataaatt aaagaaaata tcttcaagtg atactttaag tgaagaagtt
      721 aaaaaggaga taaaaaactg ggaaaacaat ttaaataatt taaatatgaa taaaaataat
      781 tctaattaat tttctcataa gaaaaatgct tatatcgtga taatgtgaaa tgtacataat
      841 tataaacaaa aaaggggctg tgacactaaa aaatagtgct acgccctttt ttatttgtaa
      901 taatcatcaa tacaaattat tttattatat gtaattattt atgtcttaac caattttatc
      961 gaaaataaca gtcttctctt ttaaagagat ttttcctact tgatatccat attcatttaa
     1021 ttccttttta tatttcaaaa acgaatggtc tatctctatc ccagcaatat cttttatttc
     1081 ttcaaaagtt aatttaaacg attgactatt gtttttctta aaatattccc ataaagaact
     1141 atatttactc atcttatatc actcctttta aaatcatatc t
//
LOCUS       DAMKMI010000286         1180 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.292, whole genome shotgun sequence.
ACCESSION   DAMKMI010000286 DAMKMI010000000
VERSION     DAMKMI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.292"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            1..468
                     /locus_tag="PC429_003807"
     CDS             1..468
                     /locus_tag="PC429_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF3849211.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
     gene            479..694
                     /locus_tag="PC429_003808"
     CDS             479..694
                     /locus_tag="PC429_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3849212.1"
                     /translation="MAIVVNLDVMMAKRKISLKELAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
BASE COUNT          431 a          137 c          148 g          464 t
ORIGIN      
        1 atgattacaa aattttttag taatatatta tcagtatttc tatattttct attatcactt
       61 ttaggagttg ctggtataat attcttatgt atgataccta tgtttttata tgattttagt
      121 tctgacaaag taattgcttt aattgcaaga atactatatt tttctgacta ctttatagtc
      181 atcttattct tgatatttat aattaatagt actaagtatt ccccattcgt acttgaaaat
      241 gtaaaaaggt ttaaagtaat gggattttgt ttattgataa acactataat tgaatgtatc
      301 aatggttata atttaaatac taataatata agaatttttg gaactgatag tggcggtatc
      361 tcaccactta tggttatctc ttttatattt gcactaatgt gctttgtaat tgcagaaaca
      421 tttgataaag ctataaaaat aaaagaagat aatgatttaa cgatatagag gtgatgttat
      481 ggcaatagta gttaacctag atgtaatgat ggcaaaaaga aaaatatcac ttaaagaatt
      541 ggcagaaaaa atagatatta caaatgcaaa tttatcaatt ttaaaaactg gaaaagcaaa
      601 agctattaga tttaccactc taaacgaaat ttgcaaagct ttagactgtc agccaggtga
      661 tatacttgaa tatgtagaag atgaaaatga ttaaaaaaga gtgtttagaa acactctttt
      721 ttattatact atatgatttt tattctttta ctatattatt gacattaata ttaactttgt
      781 ttaaagtcat aacaaaatat gttctaagaa agaaactgtt cctttatcta tttgttatcc
      841 attacctata agaactgtat cacactaaat aatactttta aattgataat tttaccacag
      901 attcctatat acattctttt ctacataata tttatttaaa ctgttagcaa aacatggaac
      961 tttttcattt acttatacct ccatttatat tattgcatag cttatagcta tgtgttataa
     1021 attacattat tgatgatttt atctaacatt tgtaatcttt tagatactct ttgaagttac
     1081 aacactgtta tactctaatt aagtaaacta aatctgatac aagttggtga ttatatgaaa
     1141 agaaaacttt caacctattt taaaggatat attttaggtt
//
LOCUS       DAMKMI010000287         1159 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.293, whole genome shotgun sequence.
ACCESSION   DAMKMI010000287 DAMKMI010000000
VERSION     DAMKMI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.293"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(11..187)
                     /locus_tag="PC429_003809"
     CDS             complement(11..187)
                     /locus_tag="PC429_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849213.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(242..339)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            565..948
                     /locus_tag="PC429_003810"
                     /pseudo
     CDS             565..948
                     /locus_tag="PC429_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          493 a          138 c          155 g          373 t
ORIGIN      
        1 caatgtctat ctaattatac ttacttacca ataccattac ttggtgtaaa caagactcta
       61 ctacattttc tccgtactct aatataaaat catctaactg tgtatctaaa aaattacaat
      121 aatcatacga actgctaata taagcagtca atacatgtgt acatttatcc attttatttc
      181 caaacatata aattattatc cccctaaaaa actttcataa aaacaaaaag aacactatct
      241 ctttggcaac tggctgacat aactcataag atatttatat aagtcttagt ccctatagct
      301 ttgcgtcact aaatttctct agttttgccg atttagtttt attctacaac taaaataata
      361 caatagaatt agttattatt caacaggatt gtttgaaagt taaataaatt tatagatatg
      421 aacattattt tacacttaaa aacagactaa acacgtagag tacctggtaa aaattaaata
      481 tagtttttgt tctaaaaagc tcctattata atttaagcta gataaatttg aagaagtgac
      541 ataaattgaa agaaggaata tattatgcta aataaaaaat taccagatac agaattaaaa
      601 attatgaaat gcatatggca tactggttat aaaactgtaa tatcaaaaga tgttgcagat
      661 gaaatagaaa aaacatatgg ttggaagcat acaactacaa ttatactttt agctagatta
      721 accaaaaaag gttttttaat atcatagaga attgggaggc atgtacacta tacagtatta
      781 ataaaagaga gagaatatct caaaatagag ggaaaaagag tttttagagg tttacataac
      841 aatcctttat cagaattaat ttcaaaatta catgatggag aagaaataac tgaagaaaaa
      901 ataatagaaa taggaagttg gataaaaagc tggaaatata aagattaata aataaaaggt
      961 agctaataat ataagtgcta ccttttttat gtggataata ttggtgataa gttgtggaaa
     1021 acttatataa aaattgtgga taaagtgttt tgctgacatt ttactgacaa aagttaaaaa
     1081 aataaatgtt atatattgta aaatgttgaa aaatagccat atatataaaa tataagtaac
     1141 actatagata aattactgt
//
LOCUS       DAMKMI010000288         1158 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.294, whole genome shotgun sequence.
ACCESSION   DAMKMI010000288 DAMKMI010000000
VERSION     DAMKMI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.294"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            330..707
                     /locus_tag="PC429_003811"
     CDS             330..707
                     /locus_tag="PC429_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF3849214.1"
                     /translation="MKEEILIEKLLRVELIVMEYLWKKDTLMPKKKIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            812..1158
                     /locus_tag="PC429_003812"
                     /pseudo
     CDS             812..1158
                     /locus_tag="PC429_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT          520 a           99 c          163 g          376 t
ORIGIN      
        1 ataaaaacaa aaataaaata tgcttttagt aacttgttaa cataacttat aatttatttt
       61 ataggcatta gcctatatag ctttgtgcta ctaaatttct ttgattctag cgatttagtg
      121 ctattctaca actaaaataa tacaatagag ctatttattg tttggtagca ttgtttgcaa
      181 attaaacaaa tttatatata tgaatattat tttacgttta aaaaaacaga ctaaatatgt
      241 agaatatttg gtaaaactta cctctagttt ttgtcttaaa aagctcctat tattaattta
      301 agttagaaga attaaggaag tgacataaat tgaaagaaga aatattaata gaaaaattat
      361 taagagtaga attaatagta atggagtatt tatggaaaaa agacactcta atgcctaaaa
      421 aaaaaattgt aaaagaaata aaacagattt atggatggca taaaagtaca ataaaaatac
      481 tactaaaaag gctagttgat aaaaggtatt tggctagata tattataaat tttcaatctt
      541 attataaaat aataatagat aataaagaat attatacttt taagaaaaag gtactaaaat
      601 ctagtaaaag cataaaaata atgcattcac ttaccactat acataaaaat gtaagtaaag
      661 agaagttaga cctattagat gagtattata gaaacttaaa agaatgatat agtaaaaatc
      721 gaatgtaatt atttacctta attcctttgt ttgtaaaact ttattgatat agaatttgac
      781 atatacataa attggtaaag ggatgtatac tatgaaaatt agtaaattac cagaagcgga
      841 attaaaagtt atgagatata tttgggaatc agaaaaggta ttaataccaa gagaaatcac
      901 caagtctatg gaacaaaaat atgaatggaa agatacaact atatttacag tattaaaaag
      961 attactaaag aagagagttt ttagaagcgg agaaaataga taagtgtaca cactataatg
     1021 tattagtaaa aaaaaataat atcagagatt ttaaacaaaa gaatttttaa agaatacaca
     1081 taaaaattca atattaagtt taatgtcagc actatgtagt aaggatgatg aagtagatgg
     1141 tgatatttta aatttatt
//
LOCUS       DAMKMI010000289         1125 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.295, whole genome shotgun sequence.
ACCESSION   DAMKMI010000289 DAMKMI010000000
VERSION     DAMKMI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.295"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            217..927
                     /gene="rgaR"
                     /locus_tag="PC429_003813"
     CDS             217..927
                     /gene="rgaR"
                     /locus_tag="PC429_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF3849215.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          454 a           90 c          184 g          397 t
ORIGIN      
        1 atattatatt tattttacta tattttttta cataatatat ctagaaaagt ataggaattt
       61 atcgtaattt aagtttaaat attaaagaga atttctataa gtagtttaat cgacaattta
      121 aatttataaa gaaatttaaa tttgaaagct agtgagtaca gtttggaaat tgaaataaag
      181 taaactaaat gatattttag atttggaggt gatattatgt tcagggtagt tatatgtgat
      241 gacgaaaaaa tacaaagaag catactaaaa gagtttacag aaaaactttt gtctgaaaga
      301 tgtctaaatt atcaaatact ggaattttcc tgtggagaag atttaatatc caaataccca
      361 gaaaaaattg atattatatt tttagatatc caaatgaaag atatcaatgg aattgaggtg
      421 gctaaaaaaa taagaaagtt tgatgaaaaa gtagaaatta tatttacaac ggctttttca
      481 gagtatgccc cccaaggtta tgaagttcgt gcttatagat atttagtaaa gcctatagag
      541 tatagtaact ttgcaatggg tgtaaatctt tgtattgata accttcaaag aaagaaggag
      601 cgttatatag ttttaaatag taaaaaagga tttaatagaa tactgataga ttctatttta
      661 tatgttgaaa ctgtaaagag agatttagtt gtacatacaa taggtagaga ttataaagct
      721 aatatgagca tgaaacaagc agaaaaatta ctgtgtgaaa atggcttttt tagatgtcat
      781 acaagtttta ttgttaattt aagaagaata gaagatctta gggataatac gattactatt
      841 aatgaacaat ttatacctat aagtaagtat agacttaaag atttaaaagt agcattaaca
      901 agcttgttat gtgatataat gtattaattt taaaaaggta attgaaaata tcgtaaaaaa
      961 gatttaatgt catcacaggg ctattgattg aaactgtctt aatatataaa atgttattta
     1021 tattaagaca gttttattat gcaaaatagc agtttataga cttttaatat ggtttaatat
     1081 gtatatgtag agtttccatt ataatagcat gattattaat tagta
//
LOCUS       DAMKMI010000290         1091 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.296, whole genome shotgun sequence.
ACCESSION   DAMKMI010000290 DAMKMI010000000
VERSION     DAMKMI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.296"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            295..975
                     /locus_tag="PC429_003814"
     CDS             295..975
                     /locus_tag="PC429_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3849216.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKVRSITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          477 a           69 c          174 g          371 t
ORIGIN      
        1 aaaaaatata gccttgaatt atataattgt atatgcttat ctttatacag taaataaaaa
       61 atcaaatgaa ataccaaata aagtgtatat cttaaaacaa tttactagag atacaaggat
      121 gctatgtgaa ttttgaagtg tgcagtttag ataataaata tatataaata ataaagaaaa
      181 tggatttata gtatttaaaa tttcttataa gggtaaaaaa tgctattaga aaatgtacta
      241 aaaatatttt tccaattctt tatagaaaaa taatgatatt taggagagat tgtaatgcaa
      301 attgatgtat tgataattga tggagataag aatagttctc aagagctgaa aaatttttta
      361 gaaggaaagg ggataaatgt ctatttagca tataattgta aagatgctat tataaaaata
      421 tttagcaaga aatttgactt aatattttta gagataattt tagttgatgg tgatggttgg
      481 gatttttgta aaaaagttag aagtataact acttgcccaa ttattcatat aacttatata
      541 aatgaaaatc aaagtatttt aaatgcttta aattctggag gagatgatta tttaataaag
      601 cctttaaatt tagaaatatt atatgcgaaa gtgaaagcta ttttaagaag aagagattta
      661 tttttaaata ataataaaaa taatataaaa ctagaagatt ataatagagc tagtggagta
      721 ataaagttag aaaataaaat gataaagtta actccaacag aaaatagatt attaaattac
      781 tttatagaga atgctgaaaa gactctaact attaaggaga tatatgaaca tgtatggatg
      841 aatgagtatt tagatgataa ttatagtgta gtggttgctg tgagtggatt aagaaagaaa
      901 atagagaaag attataaaaa tcctcaaaaa attataacga taagagaagt agggtattac
      961 tttaacaaga tataaagctt tataaacaga atgaatgata aggcatagat aatatgttag
     1021 tatattacaa gagtttttgt tctaacgata tatttgtaaa attagattta atttacttaa
     1081 ttaaattata g
//
LOCUS       DAMKMI010000291         1062 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.297, whole genome shotgun sequence.
ACCESSION   DAMKMI010000291 DAMKMI010000000
VERSION     DAMKMI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.297"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     misc_binding    53..316
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            467..1018
                     /locus_tag="PC429_003815"
     CDS             467..1018
                     /locus_tag="PC429_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF3849217.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          416 a          116 c          182 g          348 t
ORIGIN      
        1 ttttttagaa aaaagaatat tattatttta aaacaaaatt caatacaata aattaaatcc
       61 tatgacaagg aagagtagtt aaatttaggt tcagtataga gagctgagga tggtggaatc
      121 ttagcaagaa aaaatttgat gaatggccct tagaggaata aattgaaatc atagttttga
      181 gagtagactt tatcgtcagt cgcacgttat agcgaataag gtatgtaagt acctgataat
      241 gagatgtatg aaaattgaat tttatattaa tttttatata atttaggtgg caacgcggat
      301 aatctccgtc ctattagttt aggacggagt tttttatttt actgaaaatt taaaagatag
      361 aaggtggtga gaaagatgtt atgaatttca aaagctaaag atgatgatat agtaggttgg
      421 caaaaaaata aattgaaaat taaaaaaata ggaggaagta caaaaaatga aaacaaatac
      481 aaaaaaatta actttaaatg caatactttt agcgatggga ttattaatac atcaaatcac
      541 accagctata ggattaccaa tgaagccaga tgtaccacta gcaatgttat ttgtaatatt
      601 ggttttaaat agagatgatt ataaaacttg tttgatagcg ggtatagtaa caggaatatt
      661 tacagcactt acatcaagtt ttccgggagg tcaaattcct aatgtaatag ataaaactct
      721 tacagcaaat atagtatttt tattaatgaa tattagttat aatatgcctt ttataaaaaa
      781 attagctaag aaaacacaag atactgtagt agttgcaata ataatgccaa taggaactct
      841 tgtaagtgga actatctttt tattagcagc tcaagctata gttggtcttc caggggcttc
      901 atttacagct ttattcttag cagtagtatt accagctgtt ttaataaatc tagttgcagg
      961 aatattttta ttcaagattg ttagtctatc aataagaaga gttagttatc aagcttaaac
     1021 acgaaataat attttacttt ataaagcatt ataataacta aa
//
LOCUS       DAMKMI010000292         1040 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.298, whole genome shotgun sequence.
ACCESSION   DAMKMI010000292 DAMKMI010000000
VERSION     DAMKMI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.298"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            4..864
                     /locus_tag="PC429_003816"
     CDS             4..864
                     /locus_tag="PC429_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF3849218.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
BASE COUNT          372 a          115 c          159 g          394 t
ORIGIN      
        1 gtgatgttat tgaaaaaaga tagaataact ttttcagaaa ttttaatatt aattgtaggc
       61 tgtatattaa tggccatttc tcttaactta ttttttaatc cacatgcaat cgctgctggt
      121 ggtataacag gacttggagt tgttttaaat tcattattcg gtgtagaact ttggattgtc
      181 aatttacttt taaatgttcc attattcata tttgcttaca aaatactttc aaaaaaagat
      241 tgttttaaaa cggttttagg aataattttt cttaccatag cacttaagct tactgcaaat
      301 atggctacat tagatataac aaatgatatg tatcttgcaa ttatatctgg ttctatttta
      361 atgggagttg gtcaaggact tatttttaga ataaatggct ctacaggtgg aactgattta
      421 atggcactcc tactaaataa atattttcct acatttagta tacctgtttt aatgggtatt
      481 gtagactgcg ttgtagttgt tttatcaggt atagtaaata gacaggttga aattgcactt
      541 tattcaactg tggctttata cattttagta aaagttagtg acttgcttat tgaaggattt
      601 aattattcca aatcattcac tattatatct gacctttcaa aagatataag taaaaaaata
      661 atggaagatt tagatagagg tgcaactatt ctaaaaggag aaggtgcata tactggagaa
      721 aataaaaatg tacttttagt agtagttgaa aagaaagaag ttgtcgaatt gaaaaaatta
      781 gtaaaaaatg tcgaccctaa tgcttttatt ataattacag atatacatga agctttaggt
      841 aatggattta aaaaaataga ataactaaca aataaatagc aagtataatt aacatataca
      901 aattcaactt tttatatgtt atattatact tgctatttat atattataat tgttgttttt
      961 aagataaaaa ttaaatgtat caattattgt tttatacaag tatttccaaa tccgttctca
     1021 tttagatagt atgcttagaa
//
LOCUS       DAMKMI010000293         1034 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.299, whole genome shotgun sequence.
ACCESSION   DAMKMI010000293 DAMKMI010000000
VERSION     DAMKMI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.299"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            100..852
                     /gene="cwlD"
                     /locus_tag="PC429_003817"
     CDS             100..852
                     /gene="cwlD"
                     /locus_tag="PC429_003817"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF3849219.1"
                     /translation="MEYYSVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKELSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT          459 a          108 c          158 g          309 t
ORIGIN      
        1 aataggggca gaaatataat atcattacat aataatatat taataccaat aaataaagat
       61 atatcaatat taaaaaaatt taaatgtttg tactaatata tggaatatta tagtgttttt
      121 ataagtatac atatacaggg tgatgttgtg agaaagtaca taaaacatat aatttttagt
      181 tttgcaatga tatgtctagt agtagtatcc atatttgaaa ttaaaaatat ttctgaagat
      241 gttatcaagt atatgccagt aacaaacaaa actataattt tagatgctgg tcatggaggc
      301 attgatccag gtgcattaaa taaggataag agtacatctg aaaaggatat taacctagca
      361 ataacactta agcttagaga gcttatagaa tcaagtggtg gccttgtaat attaactcga
      421 gaagatgaca gtagccttta taaagaagaa aataataaaa ctacaagaca aaaatataat
      481 gagaatttga aaaatagaaa agaaataata tctaattcta atgccaatat gtttgtctct
      541 atacatctta atgcatttga acaatcaaaa tactatggag ctcaaacttt ttatcctaaa
      601 gacaaacaag acagtaaaga attatcgaag tgtattcaag aagaacttaa aagagtagta
      661 gataagacaa acaacagaga agtcaaacca agggatgata tatatctttt gaaagaaaat
      721 aatattccat cagtactgat agaatgtggt tttttatcaa atgaaaaaga gtgtaaactc
      781 ttaactgatg aaacatatca agaaaaaata gcatgggcaa tctacatagg aatacaaaaa
      841 tatttaagtt gaaataacta taaaaatagg tctaaatatg taaatagctt gtattaaagc
      901 agttaaaatg cattaatata ggctattttt attttgacaa aaaaatattt aaaataaaag
      961 ttaaaaagtt gttgacttag aataatatag atgatattat atatgagtgc ccaaaaggag
     1021 caccaaaata agac
//
LOCUS       DAMKMI010000294         1030 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.300, whole genome shotgun sequence.
ACCESSION   DAMKMI010000294 DAMKMI010000000
VERSION     DAMKMI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.300"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(121..612)
                     /locus_tag="PC429_003818"
     CDS             complement(121..612)
                     /locus_tag="PC429_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF3849220.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
BASE COUNT          384 a          148 c           94 g          404 t
ORIGIN      
        1 atttaaattt aacttatata ttttaaaagg ctaatataat atgtattttc ttctatacaa
       61 tcaaaaggct tcatatctaa gatatgtgat gacactaata ttcacaattt ttagtaatta
      121 tcacattata aaaaatatta cagctagtcc atcattagat gcttcttgta taaacttttt
      181 aagctcaatt agatttgccc atacatattc aaaaaaatca tcttcactct catcattcat
      241 aacaggataa atattatttt ctaacatctc attaatatta aatctttttc taaatgcttg
      301 ttcttccaaa ctgttcattg cttttgatag tttttttaca tcatttgctg taatcaacat
      361 gacaggaaat tctccatcaa tttccataag tatgtttcca cttagtacta atttgctaaa
      421 tatattatca tcatctccac caaaaggaca acctgtcaaa gtaaagtgaa tggcatgcca
      481 tgctttatca atattgatag tactattttc gttggcatct tcaaaaacaa aatcttgtaa
      541 attttcttct gcttgctgta atttaaaaac attctcttca cttattttag tataacatcc
      601 tatcattccc ataattacat tcctcctaag tagcgttttt ttaattgtaa cttataatta
      661 aacttagtta caataaaata tattgagaaa agtattattt catcttaaat ttaaatacct
      721 atattatcaa ctaaaattct gtgtttatag cgtaagtatt attttttcct aaaaattaaa
      781 tgaatcagag gttctatagg caatattgac tataaataag aactattact ttttaaaaaa
      841 aatttaagaa attatactgt agaatcatgc tctacttatt agctttaaat aaaaaataaa
      901 tcatgttctc aaattaaata ataaataaca ataaatttaa attttaaact ataagagttt
      961 ctgaagtgtt aataagcata ccaataatta tatgtgtaag aatttgattt aatagatatg
     1021 aataattttt
//
LOCUS       DAMKMI010000295         1003 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.301, whole genome shotgun sequence.
ACCESSION   DAMKMI010000295 DAMKMI010000000
VERSION     DAMKMI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.301"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(396..572)
                     /locus_tag="PC429_003819"
     CDS             complement(396..572)
                     /locus_tag="PC429_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849221.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
BASE COUNT          391 a          111 c          124 g          377 t
ORIGIN      
        1 cgtttttatt gaccagttat gcagttgata ttgaatattc taaatattgg tgttatttta
       61 taggtggttt aaacatatta gggggatttt atatatgatt tgtatgattt atagagaatg
      121 tataaaatag taaaggttta gagccatgtt tagtggattg cagaatatgc atatagaatg
      181 attcttgtaa attaagttag acataggaaa tatataattt gtattggatg attaatttta
      241 tggtgtatgc ttttaacttc tataatggga aagtaaagat gtactcccaa tacatcttta
      301 ctttagcaga agttatagtt acttataaag tacaaaaaat tatatattta ttagagtaga
      361 taaatctaaa aaggtttttc tagaaaaatt tagttttaat tgtatctact tactaataat
      421 aagacttgat gtaagcaggt ttcaactacc gtttctccat attctgatat aaaatcatct
      481 aactgtgtat ctaaaaaatt acaataatca tatgaactac tgatgtaagc agttagtata
      541 tgtgtacatt tgtctatttc tccaataaac atattatcca tccccctaaa aaatttagta
      601 aaaataaaaa gtcgcattct attagtcaat taacataatt tataaaattt tttagtacta
      661 gtttctataa cttaactcct taaggttaaa tattcccagt tttaccaatt tagttttatt
      721 ctacaagtat ataatactag aagatgcttc attattcaac atgcttgttt gagaatagaa
      781 aatattctaa aattataatt gtttttaaaa ataagattca agataaataa agttatatgc
      841 aactatttaa taatctttcc ataataagga cagtaggttc tattcttaaa aaactccatc
      901 tttaaattta aagaataact acaatttgaa caacaataaa atttccaatc aataaaaata
      961 tcaaaaataa taaaaataat tgaaaatgta taatttccca gaa
//
LOCUS       DAMKMI010000296          953 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.302, whole genome shotgun sequence.
ACCESSION   DAMKMI010000296 DAMKMI010000000
VERSION     DAMKMI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.302"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            273..740
                     /locus_tag="PC429_003820"
     CDS             273..740
                     /locus_tag="PC429_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF3849222.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          359 a           63 c          167 g          364 t
ORIGIN      
        1 aattaaaaaa acatagatat ttcagtaatc taaaatagtc tacaaaaata acagttttag
       61 caaaattaat aagaatacat atagatttaa acctaaaagt atgaatgtgt ctgtttttta
      121 caagattaaa atttatatta tatgatacaa ttaacttaac tatttcaata agacttataa
      181 tttttttctt tatactagac cttattgaaa cctttatagt ttaattctaa ttatatatta
      241 taaataaagt atgtgtatat taaggaggta atatgaagtt tttaaatgtt ttaatagtag
      301 ttgaagatat tgagaaatca aaaaagtttt attatgatgt tttggggtta aaggtaatct
      361 gtgattttgg agaaaatgtt gtattagaag ggaatatatc attacaagaa aaaaagcttt
      421 ggttagagtt tattaataag agtgacagtg aagttaagtt caatggaaat gatgctgagc
      481 tgtattttga ggaagattat tttgatactt ttgttgaaag attgagtaca atgagagata
      541 tagattatgt tcatctagct atagagcata ggtggggtca aagagcaatt agattttatg
      601 atttagatgg gcatattatt gaggttggag aaactatgtc atctgtgtgt agacgttttt
      661 tagatagtgg tctttcaata gatgaggtag ctaaaagaat ggatgtaact gtcgaatata
      721 ttgaatcagt tttagaatag atggattata ttaaagtaaa actatatttt gtatagaagt
      781 aagggctgta agtaaacttg tatggaaaca aatttactta cagtttttgt ttttgtaaga
      841 tacagaatat aatatttgtt ataattttat ttggattttt aaaaagtgca tgattttcct
      901 tacatattat aatagaattt aatatttatt ggatgattta aatttagaaa aat
//
LOCUS       DAMKMI010000297          951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.303, whole genome shotgun sequence.
ACCESSION   DAMKMI010000297 DAMKMI010000000
VERSION     DAMKMI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.303"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            52..408
                     /locus_tag="PC429_003821"
                     /pseudo
     CDS             52..408
                     /locus_tag="PC429_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            718..>951
                     /locus_tag="PC429_003822"
     CDS             718..>951
                     /locus_tag="PC429_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849223.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKRLD"
BASE COUNT          362 a           90 c          130 g          369 t
ORIGIN      
        1 tattttttat taatatttaa tatagttaat ttttaataat tatattatac aataacaata
       61 tataagctaa ttaaaaacct taagaagaac gatattgaca ctacttgaat tatattatag
      121 aaaagctgaa ataaataaaa tagtttgggc tagaataggt tttaatttat atctttctaa
      181 aggagttgca ttaactttaa ctgatgctgg tatagggatt tcaggacttt tggctggtgc
      241 tattcctggt ataggacagt acttgccact tatagttaga ttaattacaa tgtaccttga
      301 gtcaaatata acatctggaa aaatctttaa atttaaaaaa tacgttataa taaaggaggt
      361 gcttgtgtaa ccaaatacta cttacaatct gtagaaatca ataggtgata aaatgattat
      421 aaaatatcta ctttttgcaa ttgtttcagc attaatttta ttattttgta ataaagcatt
      481 ccaactaagt aaattacaca agttcattat attgtttgtt tcatttttta tactaacttt
      541 tgtagcatca ttcattagta gataaaaaat aagatggtgg ggttaactta ccatcttttt
      601 ctcatttaat atttgtaata ttttagatac tctttatact tgtgttactg ttatactttg
      661 attaggaaaa ttaatacaac atgagttgat gattatatta taaaggaggt atcttctatg
      721 aattttagga atatttatct tatacttatg ggattgggaa ttataatatt aactacaata
      781 atatctttaa ttgaaattaa aattggtttt tgtaatgaaa aatattttaa taaattagaa
      841 tcaaaatatg gaaatataga tagaaaaaga actattaaac tagaagtact ttatcgatat
      901 gtaacaggtt ttgaatatat agcaatagga ttatttacaa aaagattaga t
//
LOCUS       DAMKMI010000298          943 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.304, whole genome shotgun sequence.
ACCESSION   DAMKMI010000298 DAMKMI010000000
VERSION     DAMKMI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.304"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            139..393
                     /locus_tag="PC429_003823"
     CDS             139..393
                     /locus_tag="PC429_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849224.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            438..761
                     /locus_tag="PC429_003824"
     CDS             438..761
                     /locus_tag="PC429_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849225.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
BASE COUNT          391 a           75 c          152 g          325 t
ORIGIN      
        1 ttaaaaaatt taaatatata aaactataaa ggttacaatt ttggaaatag atgtaaatat
       61 atgttgtatt gtataataag agtaatataa aatctagtaa aaataatcaa ataaatttag
      121 cttatgagag ggataactat ggaaaataag gattttaaat taacaatatg tatatttgct
      181 tttgtaattg gagtagtagt attattctgt tgtataggtt taggtgaatt tagtgcagag
      241 catattattc aacaaaatgg tggaatcatg gatactgacc aatataatat ttatctagac
      301 caatctattt ctcaatatag aaattttggt tctatattag ctctgttagg aggaataggg
      361 atattgtttg ataaatatat cagaaaatac tagagattat gttttgtcta aaataaattc
      421 ttatgaggag ataaaaaatg gcaacagaaa tatttactgt aaaaattaca cccttagagg
      481 cacaaaaaat gataaaagaa aatcttgatg cagatttagt ttttagtgat tcttataatt
      541 taggagatga taaatttata ctaattacaa cttttgagaa gtattatgca agaaataata
      601 gtgatgcagg attaatagta gtttatgaaa ataccacagg aaaaacagtt attaaagcta
      661 cttcaacagg cagtgcaatt ggaattttaa aaattgattg gggtgcaggt ggaaatttac
      721 ttaaaagagt aaaaaacata cttgctgatt atattattta attattttat ctaactgata
      781 atatgtcaag aaaatctcgt aaaagaggtt aagaacattt ttataatata tagataaata
      841 agaatttgaa ggtaaagttt ttaaggtata aagaaaaata atataataaa acgaattggc
      901 tttataattc tttataaggc aaagagcaca tttctttata taa
//
LOCUS       DAMKMI010000299          869 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.305, whole genome shotgun sequence.
ACCESSION   DAMKMI010000299 DAMKMI010000000
VERSION     DAMKMI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.305"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            74..772
                     /locus_tag="PC429_003825"
     CDS             74..772
                     /locus_tag="PC429_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF3849226.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNIPTKILYGKHDELTEIGVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          334 a          110 c          131 g          294 t
ORIGIN      
        1 ctatattaca acattatttt atagtttgat taatttaata atcactaata tatcaaactg
       61 aaaggagatt tttatggaaa aaaaatattt cgaaattcat ggagttcctt caatcctttg
      121 gggaaatcct tcaagtaaat tatatattta catacatgga caatgtggca ataaagagga
      181 ggctgaagct tttgctaata ttgcagttcc acatgagtgt caagttttaa gtgttgattt
      241 gcctgaacat ggagaacgta aaagagaaaa aaattcattt aacccatggc atgttgttcc
      301 agaattagta tctattatgg aatatgcaaa gtgccactgg aatcaaattt cgttatatgc
      361 aaatagcatt ggagcatggt ttagtatgtt aagctttgaa aaagagaact taagagagtg
      421 tatttttgta tctccaattc tagatatgca acagctcatt tcaaatatga tgctctgggc
      481 aaatgtatca gaagaacagc ttaaacatga actaattatc ccaacttcat ttgggcatac
      541 tctttcatgg gaatatctac aatatgtaaa acaacatcct atcactcaat ggaatatacc
      601 aacaaaaata ttatatggta aacatgatga acttactgaa atcggtgttg tagagaaatt
      661 tgtagaaaag tttaattgca accttactat tgtagaaaat ggcaaacatt ggtttaacac
      721 accacaagaa ttaaattcat tatatgaatg gttaaatact ttagttaaat aaaaaataat
      781 tatattatta atatgattaa attatttata aatgttattt aattaatttt attttagcat
      841 tattaaaata actacataaa gaataaatt
//
LOCUS       DAMKMI010000300          825 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.306, whole genome shotgun sequence.
ACCESSION   DAMKMI010000300 DAMKMI010000000
VERSION     DAMKMI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.306"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
BASE COUNT          279 a           93 c           83 g          370 t
ORIGIN      
        1 ttatatatat tataacaatt atagcaaatt tttagggtat aatttttaaa attatattct
       61 actatcaaaa gatatgaata aatgattgta tatattatat caatttttat atttcatagt
      121 ccattttttc atcacatttt cgcttgtcaa gaataatatc tttaagagtg aagcgtttga
      181 tgtaaatctg gactttgttt ttgagacatt tttgaaatat ttattaaatc tttcgattat
      241 acttgtaata tatattatag atattactta ttttagtaaa actaaaggat gatgctacaa
      301 cattccagtt atttcccaaa attttaattt ctgaagtata ctgtttacat tatttttaat
      361 ctattttatc taaagtcgat aaggcttctt attcattaac tgacctatat acagatttaa
      421 aattacttat taattctttt gagtccttgt atgaaacata tttaaagtat ttcttatcta
      481 atgtattaaa catttttgta ttacagaatg aggataactt gtagctattg tctctttgaa
      541 ataagtcatc tgtgtttact cctaaaacta cttatatctg tagattttat aagttctcca
      601 tcttatataa tttctttgat taattgtttt cttaaattat tcataaaaaa atctccgtta
      661 cgatatttga ttatatttaa atcattgcta taaagaagac ttttattaac tttgaaaaac
      721 aaattatttt aattgctggt gattagttgc taccttcttt tgatattaat tctattagat
      781 tttatatagt tatatatctc tttctaacta agtaatataa tatta
//
LOCUS       DAMKMI010000301          811 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.307, whole genome shotgun sequence.
ACCESSION   DAMKMI010000301 DAMKMI010000000
VERSION     DAMKMI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.307"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            289..759
                     /locus_tag="PC429_003826"
     CDS             289..759
                     /locus_tag="PC429_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF3849227.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
BASE COUNT          375 a           60 c          149 g          227 t
ORIGIN      
        1 ataagatact gaagaggtag aaacaaaagt attaatagaa gatattgaaa aaataaaaac
       61 aaaagattga tagaaagtac tgaatagata gagataaaag tgttaataga agatattgaa
      121 aaaataaaag attgatataa gatactgaag aggtagaaac aaaagtgtta atagaagata
      181 ctaaaaaagt aaaaataaag gattaataaa aggtactgaa gagatagaga aaaaaatgtt
      241 aatagaagat actgaaaaaa tagaaataaa aaattaggag gaaaatttat gaaatatcag
      301 gtttcattat tagcagtgaa ggatattgaa gtatcaaaaa aattttacaa agaattattt
      361 gagcaagaaa ttgaattgga cttaggtaaa aatgtaacat ttaaaggagg atttgctata
      421 caagaagact tcgcatggct tacagggatt aatccagata caattataca aaaatcaaat
      481 aatatggaac tttattttga ggttgatgat tttgacgaat ttattgaaaa actcaattat
      541 tataaagatg tagagtttgt ccataagcca ctaaaacatg aatggcaaca aagagtagtg
      601 cgtatttatg acccggataa gcatattatt gagataggag agtctatgga agttatagct
      661 agaagatatt tatctgaggg atactctgta gaagaaactt ctaaaataat tcaacatcca
      721 attaaatttg taaagcagtg tgaaaatagt aaggaatgat tgtttaaatt ataattattt
      781 agtagaaatt aattgtaata gaactaattc a
//
LOCUS       DAMKMI010000302          799 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.308, whole genome shotgun sequence.
ACCESSION   DAMKMI010000302 DAMKMI010000000
VERSION     DAMKMI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.308"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            120..662
                     /locus_tag="PC429_003827"
     CDS             120..662
                     /locus_tag="PC429_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3849228.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          335 a           69 c          139 g          256 t
ORIGIN      
        1 aaaaatgata atcacggaga acttttacct tcttcaatta atatattatt attagtaata
       61 actttaaaat tatattacta ttatataaaa attggcttaa aagaaggtga aaaaagtaaa
      121 tgaaaatatg cataacagta ggtcatagta tactaaaagg tggtaaaagt acaggtgtaa
      181 atggtatagt tgatgaatac aattataata aaaaattagc tcctatgtta gctgagatgc
      241 ttataagtca aggtaataca gtagatgtaa taatttgtcc agaaaggcac tttatgtcag
      301 aaagagaaga atttttctat agagttccaa aagtaaacag tggtaaatac gatttattag
      361 tagagctaca tttaaataaa gctgatggaa cacaatgtgg tacagaagtg ctttactatg
      421 gcactgaagg attagaatat gctaaaagag tatctaatag acttggagaa ttatttgaaa
      481 atagaggagc taaaaaaaga gaaaatttat atatattgag aaatacaaat ccagttgcaa
      541 tacagattga aagttttttc tgtgataatg tgaatgattg taacaaagca aatgaatcag
      601 gttatgatta tatagcacga ttgattacag aaggtatatt gaataaagat ataaatatgt
      661 gagattatgg tgatttaggt attgattaat accttttaat attagatata aataaaaata
      721 caatgtatta gtataattta aactttcaaa agatagatta gtttgattat tgacaagaga
      781 tacctccttg aaaatattg
//
LOCUS       DAMKMI010000303          779 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.310, whole genome shotgun sequence.
ACCESSION   DAMKMI010000303 DAMKMI010000000
VERSION     DAMKMI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.310"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     regulatory      14..107
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            190..675
                     /locus_tag="PC429_003828"
     CDS             190..675
                     /locus_tag="PC429_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF3849229.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          345 a           67 c          126 g          241 t
ORIGIN      
        1 aattatgtta ttattcagat ataggcaata ttagataaat ctaatgacgc aaaactatag
       61 ggactaaaat ctttatgtaa taaaggtcat gtcagccagt tgccaaaaag ataataattc
      121 tttttgttaa aagtattttt agagttatat attttaataa cttaatattt ggcaagagga
      181 gggcttaaga tgatatctga attaatgtta aaaaattttc gcaaatctag catcaatttt
      241 aagactaaaa ttaaattgaa tttaagagaa aaaccagaca taaatagttt gaaattaaaa
      301 agtataccag aaggtaaaat tgttaagtta aagtgtgtag atggaatatg ggcagaagtt
      361 gagagtaatt atgacaaagg ttggttactt tataaatatt tagaaagatt aagtaatgct
      421 aaaaacaata gtaatgatac aataaaaaat agaaaaaaga attacatagg aaatataaga
      481 acaaatggat taagtttaga acttagaaat gatagaacat tagaatctaa agttataact
      541 acaatccctg atggttttaa agttgaagta tgttatagcg ttggaaaatg ggcaagagtt
      601 aatatcaata aaaatgaaaa acgatattct ggatatgtat ataatcaata catagaagag
      661 catttaagtt tataatacga tactagcaag ctttaaatat attaataaat cgcaaaaaag
      721 aatcccatta atgggattct tttttgtaga aacattataa cagaactaaa gagaataaa
//
LOCUS       DAMKMI010000304          760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.311, whole genome shotgun sequence.
ACCESSION   DAMKMI010000304 DAMKMI010000000
VERSION     DAMKMI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.311"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            200..706
                     /locus_tag="PC429_003829"
     CDS             200..706
                     /locus_tag="PC429_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF3849230.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          306 a           76 c          128 g          250 t
ORIGIN      
        1 actttataat caatatattt tatctatatt ttacacttat aaaatatgaa aactacttat
       61 tgcgaaagta attgatttgt tgtattataa tttacatgaa ttatgtagga caatcaaaag
      121 tcagtattat aagagtacat atattcaaat ttttaacaat taatttatat aattaaaaaa
      181 tgaagggtag gatgaagtca tgggttttag agaagtaaaa attgaggaat tacagtttaa
      241 tccatttact aagattggca aagaatggtt acttataaca gctggtgatt cagaaaagtt
      301 taacacaatg actgctagct ggggtggtgt tggagtctat tggggtaaaa atgttgttac
      361 aacttacata agaccacaga gatatacaaa agaatttgta gatagtaatg atacatttac
      421 aattgccttc tttgatgaaa cgtatagaga agctttaaat atttgtggca ctatttctgg
      481 tagagatata aataaaatag aaaaagcagg acttacacca tattttgtgg atgatacggt
      541 agcttttgaa gaagctaata tgattatagt atgtaaaaag ctatatcatg gtaatatgcc
      601 accagaaaac tttgatgcta aagaaaatga taaaaaatgg tatccagaga aagactatca
      661 tactatgtac atttcagaaa ttataaaagt tcttgtaaaa gaataattat aataaaaaat
      721 atatgaaaag aacttattat aagtaaatga ctgtgctttc
//
LOCUS       DAMKMI010000305          757 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.312, whole genome shotgun sequence.
ACCESSION   DAMKMI010000305 DAMKMI010000000
VERSION     DAMKMI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.312"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            231..515
                     /locus_tag="PC429_003830"
     CDS             231..515
                     /locus_tag="PC429_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849231.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
BASE COUNT          317 a           54 c          104 g          282 t
ORIGIN      
        1 taataaaaag atagtagcta tttttatata gctattattt ttttattgtt gtggatgaaa
       61 atatacttat attttactaa tatacattta aaaattacaa tttaaagcat taaaaaataa
      121 atttgtattt tgttttatat aaagtaattt gaaaaatatg gaaaaattga ttagtacatt
      181 attaattagg gtataaaatg gtgtaaatag aatgataagg ggtggatgaa atgcttgtat
      241 ataataaaag tttttatcct aatgacatat ttccaagatt agatttttca aaaataaaaa
      301 aacagttaaa attgatagat aatgacctgt cagattttgg aagcatatgt ataatagaaa
      361 aagaacatta tacgataagt gtaaacagta taggtgaaat aaatgtgtac tatgatttag
      421 agtacgaaaa taaggtgtat agaatagttt atgagattga aaagttattt aaatctcaag
      481 ttggaaggtt tagcatatct acatacagaa attgataatt aaaaaagtag aaattaaaaa
      541 acttaatact aaagatatag ataaatatat gaggtaacag ctagagagag ttgttaccct
      601 ttttaaatac taagtatata ttattcttat taattttttg taaattcact aaaagacctt
      661 tttatagaat attattttct ttttttatta aataggtaaa tcagtaaaaa tgcaattcca
      721 gttccaatta ttattccaat gatattttct ataatta
//
LOCUS       DAMKMI010000306          735 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.313, whole genome shotgun sequence.
ACCESSION   DAMKMI010000306 DAMKMI010000000
VERSION     DAMKMI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.313"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..254)
                     /locus_tag="PC429_003831"
     CDS             complement(<1..254)
                     /locus_tag="PC429_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3849232.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT          300 a          110 c           86 g          239 t
ORIGIN      
        1 ttagatatct taaacttaat gtgaagaatc cctgtaacaa gaccagcagt acaaccacat
       61 aagatagcca ttaaagttgc tactacagga gaaataccat tagtaagaga aaatgctaca
      121 atagaagcac ccattgtata gctaccatca gcagacatat ctggaaaatc taagattttg
      181 taagttatat aaacccccag tgccataatc gataaaatta aactttgcgt cattacagat
      241 ataatacctg acataaataa aacacccctt tgaaaatatt ttaaatggtt tttaaaattt
      301 ggttttaaaa aatgattttg atataaaaaa accacacagt atatataact gtatggcaaa
      361 aattcgaata agccacacag tataatccat gtggctctat attatgatga taaaataaaa
      421 actttaacaa caattactat agccaacata gatacaatcc ttaaaaatca taatcctaag
      481 gggcttgtat ctatgttatg acaatttgtc ttaacttagg tacaaaccct aactaaattg
      541 gctactaaaa aatcgtactt gtttgtttaa taaagtcttc atatttacct ctccttttaa
      601 tttaataatt ttgaaaattt tgttttctgt tttgtatagt aattagttta atacagtaaa
      661 aaataaaagt caactataaa ttttgaaaaa ttatcaaatt atcagaaaat tgagtttaaa
      721 taaaaaaatt tttac
//
LOCUS       DAMKMI010000307          726 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.314, whole genome shotgun sequence.
ACCESSION   DAMKMI010000307 DAMKMI010000000
VERSION     DAMKMI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.314"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            247..>726
                     /locus_tag="PC429_003832"
     CDS             247..>726
                     /locus_tag="PC429_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF3849233.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFYLTKKDKNHIINNLKLNIYNKDSVLYDRTEYI
                     "
BASE COUNT          323 a           51 c          112 g          240 t
ORIGIN      
        1 tactaaaaaa taatatatgg tatataatat aaaaaaagat tgataaataa ataaaaaata
       61 tattatgtta tttatttgaa tagattatcc agaagacata aataagaaaa ataatgtgta
      121 agtaaaaata ttagaattta ttaaaagcta ttacaagtat aagaaatttg aaatgattat
      181 tcaaaaattg acatgtgaaa aaaaatatga tatgctgcaa taaaagttaa tgtgaggtat
      241 gtcagaatgt ataatgttat agatcttttt gcaggtgcag gtggtttgag cttaggattt
      301 gaaatgacta aaaaatttaa tatggtggct tttgtagaaa aaaatgataa cgcagcaaaa
      361 acataccttg aaaatcatcc gagtgttaag cgttattgtg atattaaaag attagatttt
      421 caagatatat taaacagtgt cgataagata gatgttgtta taggaggacc gccatgtcaa
      481 ggtttttcta atgctaatag acaaaaaaga aaaattataa atggaaataa tgaattagtt
      541 aagctatatg ttgatgctat tgataaatta aagccaaatg tatttgtaat ggaaaatgtc
      601 aaaactatat cttcaaataa gcattcattt tatttgacta aaaaagataa aaatcatata
      661 ataaataatt taaaattaaa tatttataat aaagatagtg ttttatatga tagaactgag
      721 tatata
//
LOCUS       DAMKMI010000308          716 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.315, whole genome shotgun sequence.
ACCESSION   DAMKMI010000308 DAMKMI010000000
VERSION     DAMKMI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.315"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..634)
                     /locus_tag="PC429_003833"
     rRNA            complement(<1..634)
                     /locus_tag="PC429_003833"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          173 a          182 c          127 g          234 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccagttaa aaaggtttga tagattagaa
      661 gctatcaact tcgataattt taaaaagtta tcagctttat atattacaaa ttagat
//
LOCUS       DAMKMI010000309          716 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.316, whole genome shotgun sequence.
ACCESSION   DAMKMI010000309 DAMKMI010000000
VERSION     DAMKMI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.316"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(14..>716)
                     /locus_tag="PC429_003834"
     CDS             complement(14..>716)
                     /locus_tag="PC429_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3849234.1"
                     /translation="IICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLLKKYP
                     NNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRYLEKP
                     LKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHTEKGI
                     YTFKSSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSRYKIK
                     LLREKLNSCLVDVII"
BASE COUNT          248 a          104 c           51 g          313 t
ORIGIN      
        1 gcaaaaattt acctcaaatt attacatcta ccaaacaact atttaacttt tctctcaata
       61 attttatctt ataacgactt acaattattg aattttgatt tataaataca atattatttt
      121 ctatatctag catatttacc ttcattaaat ttataaggta gcttttatga catctaaaaa
      181 aattatgttc ttctagattt gattctattt ctttcatact actcttaaaa gtatatattc
      241 ctttttcagt atgtatagtt aaatcatttt ttaaaacttc taaatataaa atatcatcta
      301 cctttatgaa atatatcttg catttacatt ttatagataa aatagcttca tttttgtagc
      361 attcttttaa atatgatgat attgcttctt taaatagaga gtattttaag ggcttctcta
      421 aatatctata aactcctatc tcataacctt tctttatgta tttatttgat gaggatacaa
      481 atataattct tacactttca tcaaattttt tgatttcttt tgaaacatta aatccatttt
      541 catcatttaa gtctatatcc ataaatatga tatcaacatt attaggatac ttttttaata
      601 aatcgcctcc attttcaaac tctttgatat tgtattcaaa attgtaatta taaaaaaagg
      661 tatttatata atcatttaga attaccctct ctgatttttt accttcgcaa ataata
//
LOCUS       DAMKMI010000310          629 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.317, whole genome shotgun sequence.
ACCESSION   DAMKMI010000310 DAMKMI010000000
VERSION     DAMKMI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.317"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(128..607)
                     /locus_tag="PC429_003835"
     CDS             complement(128..607)
                     /locus_tag="PC429_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF3849235.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          246 a          104 c           50 g          229 t
ORIGIN      
        1 tttagtatta taatctttta attttagcat tatagtttat gtattatacc ctaatatatt
       61 ttattttaaa ttactagtta gtcactcttt tcgggttatt tatctcctat atattaaagt
      121 gtcacaccta aatagttaaa tcattatcct cttttatctt tattgccttt acaaatccat
      181 ctgcaattac aaaactcatc agagaagtta taaaatatac aaatactgga acagtaaaat
      241 aaaatccaaa tcctaaatct agaaatctca ttccttttcc agtagtacca aaagttgaaa
      301 taaactcaat tagagagttt aaaagcaaaa gatatccaat aatcctaaat ctttttacat
      361 tctcttttac aaataggcta tattcagaac tacctaatac ttctatcaat gattttataa
      421 tgataaaata tgatacacct aaaataaaca ttaatattcc ataaatagta ccttgtagag
      481 ttgcttctcc actaaaacag aggtacataa aacttttaaa tcctcctaca acaattacta
      541 atcctaaaag aaaaaataaa atttgtaata taccacttag taaatttttt ataagtttat
      601 tagacatcat gccaactcct ttaaaatat
//
LOCUS       DAMKMI010000311          611 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.318, whole genome shotgun sequence.
ACCESSION   DAMKMI010000311 DAMKMI010000000
VERSION     DAMKMI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.318"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>611
                     /locus_tag="PC429_003836"
     rRNA            <1..>611
                     /locus_tag="PC429_003836"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          126 c          179 g          134 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg c
//
LOCUS       DAMKMI010000312          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.319, whole genome shotgun sequence.
ACCESSION   DAMKMI010000312 DAMKMI010000000
VERSION     DAMKMI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.319"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>577
                     /locus_tag="PC429_003837"
     rRNA            <1..>577
                     /locus_tag="PC429_003837"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMKMI010000313          571 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.320, whole genome shotgun sequence.
ACCESSION   DAMKMI010000313 DAMKMI010000000
VERSION     DAMKMI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.320"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>571
                     /locus_tag="PC429_003838"
     CDS             <1..>571
                     /locus_tag="PC429_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3849236.1"
                     /translation="IVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVFLIISSLY
                     INIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVATVSN
                     LDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIPMIANLLS
                     FFVLYDYIINLVKISPERRTAFLVIVLLLI"
BASE COUNT          199 a           50 c           78 g          244 t
ORIGIN      
        1 aattgtttct tctattatag aaatatggtc atgtaaaaaa gtttttgatt atactagtca
       61 gtcaaagacc gacttaaaaa aactaaatat gtattttctc ttttctgttt ttctcattat
      121 ttcttcttta tatataaata taggagctaa tagtagaatc ttgatatgta tatttgtaat
      181 gttcgttttc tataagtcta actataaggt taatatatat aaatgtctgg taatctcatt
      241 tttttattgg ttaatattaa taggtataaa tggattaagt atgttattag tagcaacagt
      301 aagcaatttg gataatatga atgttttact tagtggcagt ttatatagat atgaagcaat
      361 attcttggga aagataatgt taggtatatt actttatgtt tataaggctt cttttaaaaa
      421 ttttaaaata agtaaaaaag agatatacat atgtattcct atgatagcaa atttattgtc
      481 attttttgta ctttatgatt atattattaa tcttgtaaaa atctctcctg aaagaagaac
      541 tgctttttta gtaatagttt tactattaat a
//
LOCUS       DAMKMI010000314          559 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.321, whole genome shotgun sequence.
ACCESSION   DAMKMI010000314 DAMKMI010000000
VERSION     DAMKMI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.321"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            163..270
                     /locus_tag="PC429_003839"
     CDS             163..270
                     /locus_tag="PC429_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3849237.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            272..>559
                     /locus_tag="PC429_003840"
     CDS             272..>559
                     /locus_tag="PC429_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3849238.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEK"
BASE COUNT          242 a           51 c           88 g          178 t
ORIGIN      
        1 ttacggttct tatatatgtt atttaaaaat aaatataaaa aaacttcaaa atacttgttt
       61 taagtaattg tatattttaa tattatgata ggttatgaaa aatggaagtt actcatggta
      121 gaggatatgc atatagtatg tcaaatatag acagccagta ttatggggag acatctttgg
      181 aatcctagtt atttcgttgc tactgtatca gaaaatacag aggagcagat aagaaaatat
      241 attaataatc agaaaaaaca agtaaagtga ggtggtagtg gtggaaaaag cttataagtt
      301 tagaatgtat ccaaataaaa aacaacaaga gttaattaac aaaacttttg gatgttgtag
      361 atttgtatat aataagtatc ttgctaaaag aatagatgtt tataaaaaca ataaagaaac
      421 ttttacttat aaacagtgta gttctgattt aaccaattta aaaaaagaat taaaatggct
      481 taaggaacct gacaaatttt cattacaaaa cgccttaaaa gatttagata atgcatataa
      541 aaaattcttt aaagaaaaa
//
LOCUS       DAMKMI010000315          519 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.323, whole genome shotgun sequence.
ACCESSION   DAMKMI010000315 DAMKMI010000000
VERSION     DAMKMI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.323"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            129..416
                     /locus_tag="PC429_003841"
                     /pseudo
     CDS             129..416
                     /locus_tag="PC429_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          222 a           55 c           91 g          151 t
ORIGIN      
        1 atcatttatc ataaaataaa tatttcgaaa attattataa aaaattttat aacaaaaact
       61 aaatatatgt taacatataa ataagatgtg tttaatttaa gctgatgtag aaaggagtct
      121 cttgaattat gaaagttgca atttatttac gtaaaagcag agctgatgaa gaggcagaac
      181 acagaggtga atttgaaact ttaagtaggc acaaaactac tctattaaaa atagctaaag
      241 aacaaagttt aaatgtaatt gaaataaaag aagaacttat ctctggagaa agtataacat
      301 atagacctaa gatgttagaa ttactaaatg aagttgaaga aaataaatat gatgcagttt
      361 tagtaatgga cattgaccgt ttaggtcgtg gtaatatgca agaccaagga ttgattaaaa
      421 aatagctacg cacactcgtg actttagtca tgagttagta gttaaaatag tcagcatata
      481 gggaaaccta tatgtagaga taggatagaa cctatccaa
//
LOCUS       DAMKMI010000316          513 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.324, whole genome shotgun sequence.
ACCESSION   DAMKMI010000316 DAMKMI010000000
VERSION     DAMKMI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.324"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            158..271
                     /locus_tag="PC429_003842"
     CDS             158..271
                     /locus_tag="PC429_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF3849239.1"
                     /translation="MIMGRHLWNPSYFVATVLENTEEQIGKYINNQKKQVK"
     gene            273..>513
                     /locus_tag="PC429_003843"
     CDS             273..>513
                     /locus_tag="PC429_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3849240.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQN"
BASE COUNT          216 a           50 c           84 g          163 t
ORIGIN      
        1 cttacggttc ttatatatgt tatttaaaaa taaatataaa aaaccttcaa aatacttgtt
       61 ttaagtaatt gcatatttta atattatgat aagtgatgaa aaatggaagt tactcatggt
      121 agaggatatg catatagtat gtcaaatata gacatccatt attatgggga gacatctttg
      181 gaatcctagt tatttcgttg ctactgtatt agaaaataca gaggagcaga taggaaaata
      241 tattaataat cagaaaaaac aagtaaagtg aggtgatagt agtggaaaaa gcttataagt
      301 ttagaatgta tccaaataaa aaacagcaag agttaattaa caaaactttt ggatgttgta
      361 gatttgtata taataagtat cttgctaaaa gaatagaagt ttataaaaac gataaagaaa
      421 cttttactta taaacagtgt agttctgatt tgaccaattt aaaaaaagaa ttaaagtggc
      481 ttaaagaacc tgacaaattt tcactacaaa acg
//
LOCUS       DAMKMI010000317          479 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.325, whole genome shotgun sequence.
ACCESSION   DAMKMI010000317 DAMKMI010000000
VERSION     DAMKMI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.325"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>479
                     /gene="tuf"
                     /locus_tag="PC429_003844"
     CDS             <1..>479
                     /gene="tuf"
                     /locus_tag="PC429_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF3849241.1"
                     /translation="ATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVE
                     MEVRDLLTEYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTD
                     KPFLMPVEDVFSITGRGTVATGRVERGVLKVQDEVELVGLTEAPRKVVVTGVEMFRK"
BASE COUNT          197 a           60 c          116 g          106 t
ORIGIN      
        1 gcaacagatg gaccaatgcc acaaacaaga gagcatatac tattatcaag acaagttgga
       61 gtaccatata tagtagtatt cttaaacaaa tgtgacatgg tagatgatga agagttatta
      121 gagttagtag agatggaagt aagagattta ttaacagaat atgatttccc aggagatgac
      181 actccaatag taagaggttc agcattaatg gcattagaag atccaaagag tgagtgggga
      241 gataagatag tagaattatt cgagcaaata gatgagtata taccagctcc agagagagat
      301 acagataaac cattcttaat gccagtagag gacgtattct caatcacagg aagaggaaca
      361 gttgcaacag gaagagtgga aagaggagta ctaaaagtac aagacgaagt agaattagta
      421 ggattaacag aagcaccaag aaaagtagta gtaacaggag tagagatgtt cagaaaatt
//
LOCUS       DAMKMI010000318          463 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.327, whole genome shotgun sequence.
ACCESSION   DAMKMI010000318 DAMKMI010000000
VERSION     DAMKMI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.327"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            40..>463
                     /locus_tag="PC429_003845"
     CDS             40..>463
                     /locus_tag="PC429_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849242.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLI"
BASE COUNT          191 a           45 c           60 g          167 t
ORIGIN      
        1 aatttaatac ttattaaata tttatgagga gaataaatta tgaaaataca ttcaatacaa
       61 aatttaacac tagatacgcg taaccttata aaaaaatatt ctcttacttt aaatgaggat
      121 ttaatttggg agtttaaaca tggtaaatac cacacgataa aatactttac acataagttt
      181 gcagttaagg agtctgttct tgcacttttg ttcaacatac acaaattatg ttatgctaaa
      241 ataaaatatt ttgagtcgag tttttcaaaa tacgaacctt ataaatatag ttatcaaaat
      301 gaatttgtta aatgtgaaat gtatgatatg aattttatgt ttcataaata tagtaatata
      361 atgatagatt tgagaaattt acaagaaatt aaaaccatag aagaatttag attattttgt
      421 gattacctag aaagctttga agataattta caggatttaa tta
//
LOCUS       DAMKMI010000319          456 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.328, whole genome shotgun sequence.
ACCESSION   DAMKMI010000319 DAMKMI010000000
VERSION     DAMKMI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.328"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>456
                     /locus_tag="PC429_003846"
     CDS             <1..>456
                     /locus_tag="PC429_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF3849243.1"
                     /translation="SVTGNKVGFVKIQLGDGGGSEYTPTESQTALKNVVWEGNIGNTT
                     TDETAPNCIILESLIPSSVGGFMIREIGYLDDENNLIAISKYKECYKPSIEQGAVVDM
                     KVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETKIGTVNTKIDTTKTE"
BASE COUNT          187 a           57 c           88 g          124 t
ORIGIN      
        1 agtgtcacag gtaataaagt gggatttgta aaaattcaac ttggtgatgg aggagggagt
       61 gaatatactc caactgagag tcagacagct ctcaaaaatg tggtatggga aggcaatatc
      121 ggaaatacaa ctacagatga aactgcacca aattgtataa tattagagag tttaatacca
      181 tcaagtgtag gcgggtttat gataagagaa ataggatatt tagatgatga aaataattta
      241 attgccattt ctaaatacaa agagtgttat aaaccttcta tagaacaagg tgcagtggta
      301 gacatgaagg ttaaaactgt gcttattgta tctaatgtaa ataatataga acttaaaatt
      361 gacccaacaa taatctttgc aacactcaaa gatatacaag acttagaaac taaaataggt
      421 actgttaata ctaaaattga tacaactaaa acagaa
//
LOCUS       DAMKMI010000320          438 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.329, whole genome shotgun sequence.
ACCESSION   DAMKMI010000320 DAMKMI010000000
VERSION     DAMKMI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.329"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>438
                     /locus_tag="PC429_003847"
     CDS             <1..>438
                     /locus_tag="PC429_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDF3849244.1"
                     /translation="RAMKGSAGSGKSVNVAQDYILKLGDKKYQGANLLVVRKSEATHK
                     YSTYAELTGAINRIYGKQADKYWKTTLNPLEIKSKVTGNSIIFRGVNDAKQREKLKSI
                     NFSKGKLTWVWCEEATELMESDIDILDDRLRGILTNPNLYYQMT"
BASE COUNT          179 a           55 c           86 g          118 t
ORIGIN      
        1 agagcaatga aaggttcagc agggagtgga aaatctgtta atgtagcaca agactatata
       61 ctaaagttag gagataagaa gtatcaagga gctaatctat tagtagttag aaagtcagaa
      121 gctacacata agtattcaac gtatgcagag cttacaggag ctataaatcg tatttatggt
      181 aaacaagctg ataagtattg gaaaactact ttaaatcctt tagaaattaa gagtaaagtt
      241 actggtaact ctataatttt cagaggagtt aatgatgcaa aacaaagaga aaaattaaaa
      301 tcaattaact tctcgaaagg aaaattaaca tgggtttggt gtgaagaagc tacagaactt
      361 atggaaagtg acatagacat actagatgac cgtttaagag gtattttaac taatcctaac
      421 ctatactatc aaatgaca
//
LOCUS       DAMKMI010000321          405 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.331, whole genome shotgun sequence.
ACCESSION   DAMKMI010000321 DAMKMI010000000
VERSION     DAMKMI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.331"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..215
                     /locus_tag="PC429_003848"
     CDS             <1..215
                     /locus_tag="PC429_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF3849245.1"
                     /translation="IGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSL
                     DLLSDNELKIIQGTIDGILNSRENKK"
BASE COUNT          125 a           73 c           68 g          139 t
ORIGIN      
        1 atatcggttc acacccaagt tttcaattat tccatgattt agttaccatg ttcaatatat
       61 cggttgatga atatttctat ccagcagaga aagttgcaaa aagcacagct cgccgtcaga
      121 tagaaacatc acttgattta ttgagtgata acgaattaaa aattattcaa ggtacgattg
      181 acggtatctt gaacagcagg gagaataaga agtaatcagc ttcttgtttt ccccgttttc
      241 tttttagcca tatttcttca agacttattt gttgatctgc tttgcaggaa acaaataaga
      301 gccgacaatc tataagttac aataacctac aaattatcgg ctctgcgtct tagcgtctgg
      361 ctctttgata atagttactt ttccatgctt tatattgatt aaagt
//
LOCUS       DAMKMI010000322          383 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.332, whole genome shotgun sequence.
ACCESSION   DAMKMI010000322 DAMKMI010000000
VERSION     DAMKMI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.332"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            22..303
                     /locus_tag="PC429_003849"
     CDS             22..303
                     /locus_tag="PC429_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849246.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT          166 a           26 c           66 g          125 t
ORIGIN      
        1 aaaggagagt gatttaatag tatggcatca aaacaaacac caagaaacaa tttagaaaag
       61 tttttaagaa taagagtatt atcacataca ggcgataaaa tacatataaa gttacctgta
      121 aattttgcaa agagaatgat tgagaataat gccttggatt tatttaatgg aaaagatgat
      181 gtagtagatg gtaaaaaagt tactgaaata ttagttaagg catttgacta tgatttaact
      241 ggagaagtag ttcacttgga gagaaaaaat ggagacatta ttaaagtttc aattgtacaa
      301 tagaattatt ttatatgaat tttatttttg aatagttata aataattaga aatttgtaaa
      361 aaagtataat atattattag taa
//
LOCUS       DAMKMI010000323          381 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.333, whole genome shotgun sequence.
ACCESSION   DAMKMI010000323 DAMKMI010000000
VERSION     DAMKMI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.333"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..>381)
                     /locus_tag="PC429_003850"
     rRNA            complement(<1..>381)
                     /locus_tag="PC429_003850"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           89 a          107 c           87 g           98 t
ORIGIN      
        1 tgtactaccc attgtagcac gtgtgtagcc ctaagcataa ggggcatgat gatttgacgt
       61 catccccacc ttcctccagg ttatccctgg cagtctctct agagtgccca acttaatgat
      121 ggcaactaaa gacaagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga
      181 gctgacgaca accatgcacc acctgtcacc aatgtccccg aagggaactc tccgattaag
      241 gagatgtcat tgggatgtca agcttaggta aggttcttcg cgttgcttcg aattaaacca
      301 catgctccgc tacttgtgcg ggtccccgtc aattcctttg agtttcactc ttgcgagcgt
      361 acttcccagg cggagtactt a
//
LOCUS       DAMKMI010000324          371 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.334, whole genome shotgun sequence.
ACCESSION   DAMKMI010000324 DAMKMI010000000
VERSION     DAMKMI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.334"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
BASE COUNT          165 a           32 c           36 g          138 t
ORIGIN      
        1 gtcattaaat atgccaatag ttttatatat tattgatttt attaaaaaaa gagatataca
       61 aactaataag ttgtaatctc tcaaatataa aaaaacaata aattagctca ttggattttt
      121 tagtccataa tataattata gattattcac caaacaatct atagaaattg tggtattaat
      181 gtttaaataa ccaacttttt ctaaaataac ctttatagga atatatgagt tcataagtaa
      241 agatagcgaa ataagaaacc agtttggtat gtaaaatcta gatttttaat taaattatca
      301 agattatata ttatagtatc aaaaatagaa aattaaaatt aaatttttta taataaattg
      361 tatacattat a
//
LOCUS       DAMKMI010000325          364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.335, whole genome shotgun sequence.
ACCESSION   DAMKMI010000325 DAMKMI010000000
VERSION     DAMKMI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.335"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="PC429_003851"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="PC429_003851"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="PC429_003852"
     tRNA            291..362
                     /locus_tag="PC429_003852"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAMKMI010000326          361 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.336, whole genome shotgun sequence.
ACCESSION   DAMKMI010000326 DAMKMI010000000
VERSION     DAMKMI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.336"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(12..305)
                     /locus_tag="PC429_003853"
     CDS             complement(12..305)
                     /locus_tag="PC429_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849247.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT          137 a           74 c           20 g          130 t
ORIGIN      
        1 taatttatac tttaaagatt cccatcttta tcatatttta ttcttataag aaaatataat
       61 cctattacta ctaactctat cactaaaaat actccatagg taagaattga atcaatacta
      121 tctaacccat ttctaaaaaa aattaatgct atacctgctg ctataatccc tatagcattt
      181 tttcttactg cattttgccc aactccataa aaacctggca tttttccttt tatttcgaca
      241 gcagttaaaa ttatcccaaa cactataaat aatactccaa taagcatcat aatcatacta
      301 atcatctaat tacctccttc acttaaggaa atttcaattt ctaaaatcta aaataaataa
      361 t
//
LOCUS       DAMKMI010000327          304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.338, whole genome shotgun sequence.
ACCESSION   DAMKMI010000327 DAMKMI010000000
VERSION     DAMKMI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.338"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>304
                     /locus_tag="PC429_003854"
     CDS             <1..>304
                     /locus_tag="PC429_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF3849248.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPELNAKVSYAVEKADCYTGNTIESER"
BASE COUNT          119 a           44 c           55 g           86 t
ORIGIN      
        1 ctaatgtaat ccaaaagata caacaattag tacctccaac tcatccagtt ttaggtgatg
       61 gaatattagt tttaacagat ataaaatctt ctccatatcc aggagcatca gtagtgccag
      121 attattgcaa agctactttt gatagaagat tattagttgg agaaacaaga gaaggcgtat
      181 tagctccaat acaagcttta ttagatgaaa tgatgaaaga agacccagaa ttaaatgcaa
      241 aagtttctta tgcagttgaa aaagctgatt gctatacagg aaatactata gaaagtgaaa
      301 gatt
//
LOCUS       DAMKMI010000328          293 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.340, whole genome shotgun sequence.
ACCESSION   DAMKMI010000328 DAMKMI010000000
VERSION     DAMKMI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.340"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            <1..>293
                     /locus_tag="PC429_003855"
     CDS             <1..>293
                     /locus_tag="PC429_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF3849249.1"
                     /translation="LLIELHLNASDGQGKGSEVLYYSNKGLEYATRICNKLGTVFRNR
                     RAKLDKGLYILNSSNPTAVLIESFFCDNKEDYEKAKKLGHEGIAKLIVEGVLN"
BASE COUNT          122 a           25 c           55 g           91 t
ORIGIN      
        1 ttacttatag aactacatct aaatgcaagt gatggtcaag gaaaaggttc agaagttcta
       61 tattatagta ataaaggttt agaatatgca actagaatat gtaataagct aggtacagta
      121 tttagaaata gaagagctaa attagataaa ggattatata tcttaaatag ttcaaatcct
      181 acagcagtat taattgaaag tttcttctgt gataataaag aagattatga gaaagctaag
      241 aaactaggtc atgaaggtat tgctaagtta attgtagaag gtgtattaaa taa
//
LOCUS       DAMKMI010000329          288 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.341, whole genome shotgun sequence.
ACCESSION   DAMKMI010000329 DAMKMI010000000
VERSION     DAMKMI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.341"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
BASE COUNT          127 a           31 c           23 g          107 t
ORIGIN      
        1 ttaatattta tctatgatta agcatcaatt ttattttata ctttttgatt ttaatactat
       61 acatctaaat cttatttagg gggaacaatt atttatatga tataaaaatg ctaatttgaa
      121 tgaacttgtc ttataatcag ttttagaaaa tacaccattc tacaaagtat gaatcaaaag
      181 gtgaaaaaat actaaaaata ataaatcaac atcaatttta atcaatatat gaaaaactta
      241 taccaagtat gcataaaaac taatataaat attattttaa caactaat
//
LOCUS       DAMKMI010000330          252 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.342, whole genome shotgun sequence.
ACCESSION   DAMKMI010000330 DAMKMI010000000
VERSION     DAMKMI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.342"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
BASE COUNT           86 a           27 c           23 g          116 t
ORIGIN      
        1 aaaaaaagta caaattatta tatttttctt tataaacttt ggtttaactt attcattagg
       61 tttattattg tattataata aatttattga tccagaaaac tttgcatcat tcatgatgat
      121 attacctcta tcttcagttg ctattgcaaa attttatact gaaggattaa ataatgatat
      181 gtatgaattt tattcactaa tcatactatt ttttcttact tattcgttat taattgttct
      241 taagttatta aa
//
LOCUS       DAMKMI010000331          240 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.343, whole genome shotgun sequence.
ACCESSION   DAMKMI010000331 DAMKMI010000000
VERSION     DAMKMI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.343"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(<1..>240)
                     /locus_tag="PC429_003856"
     rRNA            complement(<1..>240)
                     /locus_tag="PC429_003856"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           54 a           71 c           55 g           60 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
//
LOCUS       DAMKMI010000332          237 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.denovo.344, whole genome shotgun sequence.
ACCESSION   DAMKMI010000332 DAMKMI010000000
VERSION     DAMKMI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.denovo.344"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
BASE COUNT           90 a           32 c           31 g           84 t
ORIGIN      
        1 gcctgtggag aattagtaag acatatttta gtatgcaaaa ttctatgaag caggaaccct
       61 gtgactttag tcatgggagg ttcagtttag cttaaaactt acttatctat ttcagtttct
      121 attagaatta ttattagact cattaaaata aaagcactaa tataactaat aatatatgtt
      181 aaattcatta actctacata aagttttaca atctaaatga aatacaataa tctgcat
//
LOCUS       DAMKMI010000333        38134 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.guided.021, whole genome shotgun sequence.
ACCESSION   DAMKMI010000333 DAMKMI010000000
VERSION     DAMKMI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.guided.021"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            481..1683
                     /locus_tag="PC429_003857"
     CDS             481..1683
                     /locus_tag="PC429_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF3849250.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            1688..2458
                     /locus_tag="PC429_003858"
     CDS             1688..2458
                     /locus_tag="PC429_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3849251.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            2604..4025
                     /locus_tag="PC429_003859"
     CDS             2604..4025
                     /locus_tag="PC429_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF3849252.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            4291..4773
                     /locus_tag="PC429_003860"
     CDS             4291..4773
                     /locus_tag="PC429_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF3849253.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            4828..5607
                     /locus_tag="PC429_003861"
     CDS             4828..5607
                     /locus_tag="PC429_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF3849254.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            5917..6363
                     /gene="lspA"
                     /locus_tag="PC429_003862"
     CDS             5917..6363
                     /gene="lspA"
                     /locus_tag="PC429_003862"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF3849255.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            6332..7279
                     /locus_tag="PC429_003863"
     CDS             6332..7279
                     /locus_tag="PC429_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF3849256.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            7639..8172
                     /gene="pyrR"
                     /locus_tag="PC429_003864"
     CDS             7639..8172
                     /gene="pyrR"
                     /locus_tag="PC429_003864"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF3849257.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            8456..9667
                     /locus_tag="PC429_003865"
     CDS             8456..9667
                     /locus_tag="PC429_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF3849258.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            9966..11618
                     /locus_tag="PC429_003866"
     CDS             9966..11618
                     /locus_tag="PC429_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF3849259.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(11833..13608)
                     /locus_tag="PC429_003867"
     CDS             complement(11833..13608)
                     /locus_tag="PC429_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF3849260.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            13904..14938
                     /gene="ltaE"
                     /locus_tag="PC429_003868"
     CDS             13904..14938
                     /gene="ltaE"
                     /locus_tag="PC429_003868"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF3849261.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            15225..16055
                     /locus_tag="PC429_003869"
     CDS             15225..16055
                     /locus_tag="PC429_003869"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF3849262.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            16059..16940
                     /locus_tag="PC429_003870"
     CDS             16059..16940
                     /locus_tag="PC429_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF3849263.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            16970..17587
                     /gene="gmk"
                     /locus_tag="PC429_003871"
     CDS             16970..17587
                     /gene="gmk"
                     /locus_tag="PC429_003871"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF3849264.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            17590..17856
                     /locus_tag="PC429_003872"
     CDS             17590..17856
                     /locus_tag="PC429_003872"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF3849265.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            17861..19060
                     /gene="coaBC"
                     /locus_tag="PC429_003873"
     CDS             17861..19060
                     /gene="coaBC"
                     /locus_tag="PC429_003873"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF3849266.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            19299..21824
                     /gene="priA"
                     /locus_tag="PC429_003874"
     CDS             19299..21824
                     /gene="priA"
                     /locus_tag="PC429_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF3849267.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            21842..22282
                     /gene="def"
                     /locus_tag="PC429_003875"
     CDS             21842..22282
                     /gene="def"
                     /locus_tag="PC429_003875"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF3849268.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            22297..23226
                     /locus_tag="PC429_003876"
     CDS             22297..23226
                     /locus_tag="PC429_003876"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF3849269.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            23236..23988
                     /locus_tag="PC429_003877"
     CDS             23236..23988
                     /locus_tag="PC429_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF3849270.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            24057..24758
                     /locus_tag="PC429_003878"
     CDS             24057..24758
                     /locus_tag="PC429_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF3849271.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            24820..26145
                     /gene="rsmB"
                     /locus_tag="PC429_003879"
     CDS             24820..26145
                     /gene="rsmB"
                     /locus_tag="PC429_003879"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF3849272.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            26153..27184
                     /gene="rlmN"
                     /locus_tag="PC429_003880"
     CDS             26153..27184
                     /gene="rlmN"
                     /locus_tag="PC429_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF3849273.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            27204..27956
                     /locus_tag="PC429_003881"
     CDS             27204..27956
                     /locus_tag="PC429_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF3849274.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            27973..29988
                     /gene="pknB"
                     /locus_tag="PC429_003882"
     CDS             27973..29988
                     /gene="pknB"
                     /locus_tag="PC429_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF3849275.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            30096..30833
                     /locus_tag="PC429_003883"
     CDS             30096..30833
                     /locus_tag="PC429_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF3849276.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            30931..31815
                     /gene="rsgA"
                     /locus_tag="PC429_003884"
     CDS             30931..31815
                     /gene="rsgA"
                     /locus_tag="PC429_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF3849277.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            31820..32491
                     /locus_tag="PC429_003885"
     CDS             31820..32491
                     /locus_tag="PC429_003885"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF3849278.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            32500..33141
                     /locus_tag="PC429_003886"
     CDS             32500..33141
                     /locus_tag="PC429_003886"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF3849279.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            33272..33901
                     /locus_tag="PC429_003887"
     CDS             33272..33901
                     /locus_tag="PC429_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF3849280.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            33956..34612
                     /locus_tag="PC429_003888"
     CDS             33956..34612
                     /locus_tag="PC429_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF3849281.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            35027..35743
                     /locus_tag="PC429_003889"
     CDS             35027..35743
                     /locus_tag="PC429_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3849282.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            35718..38051
                     /locus_tag="PC429_003890"
     CDS             35718..38051
                     /locus_tag="PC429_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3849283.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
BASE COUNT        15249 a         3781 c         6940 g        12164 t
ORIGIN      
        1 ggtaaatata cattataata atttattgtc aaacatatta ttttaaatat gggatttatt
       61 tactttatcg atatacggct atataataaa tatataacag agagatgata atgtgtgttt
      121 aacacaatta aatcaaatga gatatttgaa caaagaatag tataaaattt ttaaagaaat
      181 atgttgtatg agtaagaatc tacataatat tgtattatat gatattcgaa agtattattt
      241 ctaagaaaag agatttctaa agtataaaat taaatactag aattgtaaag ttaataataa
      301 ctacaagtta ttacaagctt atgctagcta gtattagttg ttaatagaag tacaactatt
      361 tcttatttaa ttaaaagatt aaatgtggaa agttttgtga taggattact tcaaatatgt
      421 aatttataac aaccataata aatttagagt gtgtaaaaat ttatttttta aagggagatt
      481 atgaaaacat caattttaat cgcattaata gaaaagacaa gccttattgt agtattgttt
      541 cttctaataa caaagttaaa aatatttaag cagatatttc aaaaggaaga atatagtttt
      601 aatgacttgg tctgtatagc attagttttt acttttttag ccatatttgg aacttatagt
      661 ggtataaatt atatggggtc aatagtaaac actagaataa tatctatagt atcaggtggc
      721 attttatttg gtccgatggt aggtattaca gcaggagttt tttcagggat tcatagatat
      781 tttatggata ttggggggat aacatctgtt ccatgtcttt tatcaagtat actagcaggt
      841 gtattatcag gatttctata taagaggata ccaaaacaac atagagtaat gtatggaata
      901 ttggtaggca tgatttctga aagttttact atattactta tatacttaat atcatatcca
      961 cattctttag caattcaaat aatcggtgga atatatttac ctttaatagt tgggcagatt
     1021 ggaataggat ttgttatatc tatagtagag gggattgaga aagataaaaa agatatagaa
     1081 gcgagaaata aagcagaaat aaaagcactt caaagacaaa ttaatccaca ttttttgttt
     1141 aattccctaa acacaatagc ttcatttata agatttagtc cagataaggc tagagagtta
     1201 ataataaatt tatcaactta tctaagatat aatcttgaat atagtgataa cttaattgat
     1261 ataaataaag agattgaaca agttaaatct tttgtagaga tagaaaaagc taggtttgga
     1321 gaattactaa ctgtatctta tgatatagaa gatgtaaata taaagattcc aagtttgatt
     1381 atacaaccat tagtggaaaa tgcaataata catggtattt tagaaagtgg tagagctggt
     1441 gtagtgaaaa tatccataaa aaagttaccg tattcattag aaaatactgt gagaatatca
     1501 atagaggata atggtatagg tataagtgag gaaattataa ataatgtata tcaagataat
     1561 atgccagaaa ataagattgg gctttataat gtacatttaa ggcttaaact gatgtatgga
     1621 agagggctta atataagaag aatagataat gggactttga tagtatttta tgtaaaggag
     1681 tagaagtatg agagctataa tagtggaaga tgaatttcca gcaagaaagg aattgagata
     1741 ttttattgaa aataaaagtg gaatcgaggt agtaagtgaa tttaccaatg gtatagaggt
     1801 gttagacttt attcaggaaa ataaaataga tgtaattttt ttagatataa atatacctca
     1861 tcttgatgga atgctacttg caaaaacatt aaatcagttt aaatcaagac ctaaaattgt
     1921 ttttataaca gcatatgaaa gttatgcagt agatgctttt tctttggatg tatttgatta
     1981 tatattgaaa ccatattctg aagagagaat tatttctatg ctgaataaat tagaaaaaag
     2041 tgagatgagt gatattgaac tatttaatgt aaatagtaat ttatacaaat ataagaagga
     2101 agcagtaaat caggagatag aagaaataac acataaaatt agcctttgga aaggtgataa
     2161 attagttgta atagatattg atgatatata ttactgtgaa gctaatgaaa ggcagacatt
     2221 tatatatact gagaaagaaa agtttatatt aaaagaaggc atatcagaag tcgaaaatct
     2281 tataaatgat aaaacatttt ttagaacaca taggtcttat atcgtaaact taaccaaagt
     2341 aaaagaaatt ataccatggt ttaataatac atacatatta aaattaaaaa atagtgatta
     2401 tgaagtaact gtaagtagaa gcaaagtaaa agaatttagg ctgttaatgc atatataaag
     2461 tcatttcatg tacttaaata tgcacttaat gtaattaata tttatattag cataaaaaag
     2521 tattactatt tgaactggaa atagttttct taaactcata aaaatttata aagcaattat
     2581 ataaaaacta aggggatgaa tttatggtaa gctttttagg ttcaatagta gtacttatag
     2641 taggttattt tgtctatggt acttttgtag aaaaggtttt tggaaccaat gacaaaaatc
     2701 aaactccagc tatagcttgt agggatggag tagattatgt gccaatgaaa tggaagagaa
     2761 tctttttaat tcagtttcta aatatagcag ggttaggtcc gatatttgga gctatacaag
     2821 gagcattatt tggaccatca gcatttttat ggattgtgtt tggaacaatc tttgctggtg
     2881 gagtacacga ttttgcttca ggatatctat caatgaaaaa caaaggtaca tctgcatcag
     2941 agttggtagg cctttatcta ggaaatggtg caaaaatagc aatgagaata tttagtgtta
     3001 ttcttttagt attggttgga gttgtatttg taacaggtcc agcaggatta ttaaagacat
     3061 tgacaggtgt aaacgcacaa atatgggttg gagtgataat actttattat attatggcta
     3121 ctgttttacc aatagataaa ttgattggta aaatatatcc tttatttgga gcagcattat
     3181 taattatggc agtaggtgta gctggaggat taataataaa ggggtataac ataccaaata
     3241 taaatttaca aaatatgaat ccaaatggaa cacctttatt cccatactta tttataacaa
     3301 tagcatgtgg agcaatatca ggttttcatg caactcaatc accacttatg gcgagatgtg
     3361 ttgagaaaga gagtgaggca agaccagtat tttatgggtc aatggttgca gaaggtataa
     3421 tagctttagt ttgggcagca gcagccatgt catatttcca tggcatacct caacttaatg
     3481 tgatatttaa tgatggtgga gcagcaacag tagtaaatac agtatctgtt ggattaatgg
     3541 ggcctattgg aggagcttta gctatacttg gagttgtggc atgtcctata acttctggag
     3601 atacagcatt tagaagtgct aggctgacaa tagcagatgc aatgaattat aatcaagatg
     3661 cagtcaaaaa tagattttta atagctttac cattattcgc agttggtgtt gctcttacat
     3721 ttatagattt taatataatt tggagatatt tctcttgggc aaatcaaaca cttgcaatga
     3781 taatgttgtg gactggttct gcatatttag tcaaagcaaa taaaaaccat tatataacta
     3841 cattaccagc actttttatg actgtagtaa catttagtta tataatgcaa gcaaaagaag
     3901 gatttagact accagttaat atatctaatg gaataggtgt tattgtagca ataattcttg
     3961 gtcttttatt ctttaaaaaa gcaaaacata ttaaagaaca aaataatcat aaattggcaa
     4021 gttagtaaaa taataactat aaaaattaaa aaattgtatt taaattatgt tcaaagaaga
     4081 gactatctca aaatagagat aagtctcttt tttattgctg aaaggataaa aatatatttg
     4141 atttcatata aaaaagaggg ttaaatgaac taaaaatttc attttgagac attccatttt
     4201 tcgaatcaaa tcttattttt attgtatgtt taatttactt ttccacatac tatttttatg
     4261 atgtaaatat taaaattaga ggtgttaaaa atggatacta aaaaatataa agataaatta
     4321 ttgaaagaaa gagaaaattt aactaattta gtagaggata tgaaagataa tactttattt
     4381 ggagatacaa ctaaacatac aagtgaaaag tattcatcag gagaattatc tagttatgat
     4441 aatcatatag gagacatggg aacagattta tatatgcaaa atatgcaaaa cagtctaata
     4501 aatcatgaag agggaagatt gtatcaaata gacttagcat tatctaaaat agagaatgga
     4561 acatatggga tttgtgattt atgtcataat caaatagatt tagaaagatt ggatatattg
     4621 ccagatacaa gtttatgtaa tgattgtgca aagaaagaaa ataatttatt aggtgatgca
     4681 atgcaaaacc ctgatgtaga agattctaac ttttattcag aagacctaac aaatttaact
     4741 gatttaaata aaaatggaat attgagagat taaaaaactg acattataaa atatattgct
     4801 agaatgaatt agaaaagagg taatgacatg aagaaaataa tatggggtat atgtatatgt
     4861 ttattatcta tagtaggtgt atatacatat aatcataata cacataatca agatagttca
     4921 aaagaagcat atagtttaaa tgacaatata aaagacgatt atgaagatgt aataataaaa
     4981 aaaggaaatg aggatgaaaa aattttagca ttaacttttg atgatggtcc agatgaggat
     5041 tttacaccac aagtacttga tatattaaag aaaaatgatg taaaagcaac tttttttgta
     5101 gttggagaaa aagtagaata taacaaagaa ttattaaaaa gacaatatga tgaggggcat
     5161 gaaataggga atcatacatt tacacatata aatgttgcaa aaaatagtta tagtagtgtt
     5221 gaaaaagaaa taacagatac gcaaaatatt ataaaaagtg taataggagt agaaccaaag
     5281 atatttagac ctccatatag agccatgagt aaaagtgtct gtgatattat agtttctaaa
     5341 ggcatgaata taattctttg gtcaaactta gaccctagag attggtctaa ccctggagta
     5401 gatgcaatta taaatactat ccttacaaag gtccaaaatg gtaatataat acttttacat
     5461 gattataaca ctaaaagaaa tgataaatca caaactatac aagctttaga agtagtaatt
     5521 cctaaattaa aagagagagg atataaattc gtaacagtat ctgaacttat acaacattta
     5581 gataaaaata agcaagtgca aaagtaaaaa tatttgtttg aagattagtg gtgattgtaa
     5641 gtttaaatta cagacatcat taatctttaa tctataaaat acacaaaaaa tattgattag
     5701 tatggtattt ttaaattttt tatacataat gatatgattt taaatatgta gaaatctaca
     5761 atataaaaaa agtagtacta ttttaaattt tgttctagct ttattaagaa aatacaattt
     5821 taataaagtt gctctttaga gcattttttt tgtataataa taatagtgtg cttcaaataa
     5881 aattggtttt taaataattt atagggggaa aaatcattgc tatatatatt aataataatt
     5941 ctactcatag gtttagacca actgtctaaa atatgggtat tgaataattt ggtggatgta
     6001 tcaacaatac caataataaa taatgtattt catttaactt atgtcgaaaa tagaggtgca
     6061 gcatttggat tattacaaaa taatcaatgg atatttataa ttgttgcatt acttgcaaca
     6121 gtatttggac tatactatct taatacaagg aaagtacata tatttggaag gttgggaatt
     6181 atattaatta tatctggtgc attgggaaat ctaattgata gagtacgatt aggttttgta
     6241 gtagattact tcgactttag aattatatgg gaatatgtat tcaatatagc tgatgtattt
     6301 gtagttgtag gaactgtgtt tttatgtata tatgttttat tttttgaaag taaaagtagg
     6361 tgatataatg gatgaagtaa aagagttttt agtacttgaa gaggaagaag aagttagact
     6421 tgatatttat ctagcagaac aattaggtga tatgtcaaga agttatatac aaaaaattat
     6481 aaaagatgga aaagtaaagg ttaataataa agtagaaaaa gctagatatc tggttaaaga
     6541 ggaagataat attgttatag agataccaga acctaaagta ttagaagttt tacctcaaga
     6601 tattccaata gatattgtat atgaagatga tgatgtctta ataataaata aacctcagga
     6661 tatggttgtc catcctgctc ctggaaacta tgaaaatacg ttagtaaacg ggattttata
     6721 tcattgtaaa gataagttgt catcaataaa tggcgttatt agacctggta tagttcatag
     6781 aatagacaaa gatacttctg gtcttcttat gatagctaag aacaattatg cacataattt
     6841 tctagcagaa cagttaaagg aacatactat aactagggag tatgaattta tatgctatgg
     6901 tgtggttaaa gaagataaaa taactgttga taaacctata gggagaaatc ctaaggatag
     6961 attaaaaatg gctgtagtaa aagatggaaa aaatgcaatc actcactttg aagttgtaga
     7021 aagatttgat aagtttacac atatgagagc taggcttgaa actggtagaa ctcatcaaat
     7081 aagagttcat gctttgtcta taaatcaccc acttttaggt gattatgtat atggtccaaa
     7141 agaaactaaa tttaaattaa aaggacaaac tttgcatgct aagaagttgg gatttataca
     7201 tcctactact aaggaatata tcgaatttaa ttcagaatta cctgagtatt ttaaggaagt
     7261 tattaaaaag ataaaataac tatttgtata atacttataa atcatttttt gtttttatat
     7321 aagtaaataa atttagtgaa atttaaataa aatatcttat attaaagtat tttgtttaaa
     7381 aaagcttgaa aaatttgaaa tataaatata taatttgatt tgatagatta aaaaattatc
     7441 tttaaacgat acagagatat cgtaagacaa ttgctaaatt gcactgtata ttttttatat
     7501 aagagcttct tgtcttgtga tgagaagttt ttttgttgtt taaattgaaa ttttaaataa
     7561 aaataaattt ataaaaaatt aaaagaaagg agtagatttg tcaaatagat atattatatc
     7621 tttgacaata attatgatat ggtagaaaaa gctcaattaa tggatgaaaa ggcaatagca
     7681 agagcaatta ctagaataag tcatgaaata attgaaagaa ataaaggtgt agaaaatcta
     7741 gttttagttg gaataaagac tagaggtgtg ccaattgcaa atagaatttc taagaaaata
     7801 gaacaaatag aaggaacaaa agtagataca ggagatattg atataacttt gtatagagat
     7861 gatttggaaa aaattcatgt tgaacctgta gttaaaggaa cttatttaga ttttaatgta
     7921 aatgataaga cagtagtttt agttgatgat gttttataca ctggaagaac tgtgagagct
     7981 tcactagatg ctattataga tataggaaga cctaaatcaa tacaattagc agtgctagtg
     8041 gacagagggc atagagagtt gcctataaga gcagattatg taggaaaaaa tgtacctact
     8101 tctagacatg aaattatatc tgtcagttta ttagaaattg atggagaaga ttcagttaca
     8161 atcaaagaat aattattttt aagtattata taaaagctta gcattgaata gtcagggagg
     8221 aggataaagc ttacatagat tgcacaaaat aaatattata attttatgga tgtgagtctt
     8281 aatgtagttt ttattctcct ttatttgagt ttttgatgtc gtaaaatgta aaataatgat
     8341 aaattatata ataagtatat agaggttaaa aaatagaatc tagggaatga taattaatta
     8401 aaagatatta attaaacaga ggatttattt atatataata attttggagg tttttatgaa
     8461 aaaaacgata atgtctttac aacatctatt agccatgttt ggagcgacag tattagttcc
     8521 cattttgaca ggttttaatc cgtcagttgc aattttctgt gctggtgttg gtacattgat
     8581 atttcatttt tgtacagaag gaaaagtacc tgccttttta ggttcaagtt ttgcgtttat
     8641 accagttata ctagcagcta aagaggcata tggtggagat ttagcatatg ctcaaggtgg
     8701 tataatagta gcaggattaa tttatattat aatgtcgatt atagtaaaag tagttggtgt
     8761 aaataaaatt aaattatatt ttcctgcaca agttacagga gctatgatag tagttatagg
     8821 attaaattta ttgccaacag cttttagtat ggcttcagcc aattttgtga tagcatttat
     8881 aactttagca atagcaattt taaccaataa gtttggtaga ggatttataa aacaattagg
     8941 aatattaata gcagtttttt ctgggtatat aatctgtttg atattaagat tagtagatat
     9001 aactactatt acagaagcaa gtttatttgc aatacctaac tttacagttc ctaagttcag
     9061 tttaggagca atagtgatta tatctccagt tgtattagct gtatttatgg aacatatagg
     9121 agatatgact actaatggtg ctgtagtagg taaaaacttt atagaaaatc ccggattaaa
     9181 tagaacatta cttggggatg gatttgcgac tatagttgca gggtgtttag gaggaccagc
     9241 caatacaact tatggagaaa atacagcagt acttgctata actaaaaact atgacccttc
     9301 aatacttaga cgtacagcaa tatttgctat attgcttgca tgtgtaggca agtttggtgg
     9361 atttcttcaa agtataccag gttcagttat gggtggaatt agtataatgc tattttcgat
     9421 gataacttat gtaggattaa aaactataag ggacagttct tgtgtagaaa gtaaaataaa
     9481 tatattaatc atagcagtta tattattaat aggtcttgga acaacatatt tatctaataa
     9541 aggtatttct gttggaatcc ctataacaag tacagtaaag ataactggtc ttagtttagc
     9601 tgcaattgtt ggaattgtat taaacagaat tttaaataat caggacttta aagttgaaga
     9661 agaataaatt taaagaataa gttgtatatt gaataactaa aattagttaa ttttctatag
     9721 acatttgtaa ttaatgttga tataatgatt aaaaataaat gaaagaaagg gcaaggtaac
     9781 taggacaatg agaatataat ctatttttta taatttaaag tattgtaatt tttgaaaagc
     9841 ctattttggt ggctttattt gcaatgctta aattaaggag agtagattat agtctatacg
     9901 tttatttgtt atgcctattt atagaatctc tccttatatt taagtgtgtt aaggagggat
     9961 tttttatgtt gttagtaaaa gtagagaatt taaagaaata ttatgcagat aaattaattt
    10021 tggatataga taaacttgaa atattagaaa atgacaaaat aggtctagtt ggttcaaatg
    10081 gtcaaggaaa aacaacatta ttaaaagcaa tattgggaga aatagaaatt gatgaaggat
    10141 atacttacct tacagaaagc tattcttata taagtcagag tgaaaataat attgaaacat
    10201 gtagtcatag taaggagaag agtcttttaa atgctccaga taaatttgaa gaacacttat
    10261 caggagggga aaaggttaag ttaaaaatag cagatgcgct gagtaataag aaaaatatca
    10321 taatagctga tgagccaact tcaaacttag ataaaaaaag cattggggtt ttggaagata
    10381 tgtttaaaag gcatgaggga gcattattat taatatctca tgatagacgt tttctggatg
    10441 agttatgtac aactatatta gaactagaag atggaaaact aaaagcttac aaaggcaatt
    10501 acactgatta tttaatgcaa aaagatgaag aagtaaaaag agctgatttt gagtatcaag
    10561 aatatgttaa agagaaaaaa aggcttgaaa aagctctttt atataaaaaa gctttaagtg
    10621 atggcataag aaaaacacca aaaagaatgg gtaattcaga agctaggttg cacaaaatgg
    10681 gtggtcagac taataagaaa aagttggact caaatgtgaa ggctataaaa agcagaattg
    10741 ataaacttga ggtaaagaat aaacctaaag tttctaaaga aatgaacatt aagatgcaag
    10801 atggtatgga gataattagc aaaaatctag tagaggtaaa agatatgact ctaaagttag
    10861 aaaataagct cctgttagat aatgtttcct ttaagataaa aagaggtaaa aagatagcat
    10921 tgttaggtga taatgggtgt ggaaaaagta ctctgataaa agaaatattg gctgataaaa
    10981 atgacaatat aaaaataaat aacaaggtaa aagtaggtta ttttgaccaa aatcaaagtt
    11041 tattagatga ggaaaagagt gttctataca atactaaagt taatagttca tttgatgaat
    11101 cttttataag gataaactta agcctatttg ggtttaaagg tgatgatgtc tacaaaaaag
    11161 taaaagtact tagtggtgga gaaaaggtaa aaatagcact atgtaaaata atattggaag
    11221 ataataattt tttagtattt gatgaaccaa caaattactt agatataaaa tctatggaag
    11281 cactggaaaa ggcactgata aatactgata aaactatgct tatagtgtct catgatagag
    11341 tatttgtatc acatatttgt aactatatta tagagataaa agatgctaaa ataagggagt
    11401 ttgattgtaa ttatgacgaa tatacaatta gcagaaataa aaaaactcct agtagagaaa
    11461 accaaatcaa aaaagaaaat cttctagtat tagaaaatag acttacaact gttatatcta
    11521 tgttatctat agaaaaagat aatctaaaaa aagaattgta tgaatcagaa tataatgaat
    11581 tactaaaaca aatcaataaa ttaaaaaata gcttttagtg ttaacttttt aattacatct
    11641 atataagaag actgaaaata tttgttggga atataagtat agttattaaa acagaaaagt
    11701 attttgttag aaaattaaaa gtaagctaat tagaaagtgg ccttattatt gtactttata
    11761 agaaggccac tatttttatt tagaatgata atgtgtagtt gtctaacatt tattttgtat
    11821 ttagtgcaga atttattcag atttaacctt aagcttggct actgtttctt cttcaggagt
    11881 aacataaata gtactgttag tttcataaat aaccatacct ggtttagaac cactaggttt
    11941 tttcacattt tttctcttag tatagtctac aggcacctga gaagaaagtt ttgacttact
    12001 aaagaatgct gctagcatag ctccttcaaa tacagtatta tcagggactt ccttaccagc
    12061 acactttatt attacatgag aaccaggaat attttttgta tgcatccata agtcatcatt
    12121 atcagcaagt cttagtgtaa gatagtcatt ttgcttattg tttttaccaa ccaatatttt
    12181 aaatccatca gaagacacaa attcatgagg ttttgtagat gggatagtat cctttttact
    12241 gtttttcttt tgagtcttga tgtatccaac tttagcaagt tcttctttta tatcttgtaa
    12301 ttcagctaaa ttttcacagt tttcaatact taaaattatg ttttctaagt aatctatttc
    12361 ttccttattt aaggaaattt gatgagatat ctcgacttta gcatgtttca ttttgttata
    12421 ctttttaaag tatttttgag cattttcaga tggtgtcaag tttttattta attctattgt
    12481 tacatcatta caattttcat catagaagtt agcaacttca atactttcca tacctttttc
    12541 taccatataa atatatgaag taatcaattc tccttttatc ttataaatgt ctgcattttc
    12601 ggattctgat aattcttctt cttgtttttt aagtttatta taaagtctat caagcttaac
    12661 agatatactc ttctttaaat cagatgaacg ttgattaatt ctatctttaa tatccttagt
    12721 tctatagaaa tcttccagaa tacgagacat actatcctta tttatatatg ataaatcgct
    12781 aaataatgta aggtttatac aactgaaatc aactacctta tccacctttt tatctataat
    12841 aatgcaagga gagtatttgt tagaatttat atcattgaat aaattacaaa atactttgtg
    12901 taaagcatca aattgttcat cagatatatc ctttatgata gcattttgat ttacacctgc
    12961 tctgtagcaa atttctttag ctataatagg gcttatacct agaaatttag aatatataga
    13021 cttaaagata ggtccttcca attcctctag gtttttaata aacaaatcct tagatatatc
    13081 atcaagaggg ttgagtttgt tttgttctgg tggcaatgaa taatcatgac caggtaaaac
    13141 ttgacgaact ctacttatgc taaaagggac tctttttata gaatctataa ttttattttc
    13201 ttcaccatga gttattatta tgttgctatg tcttcccata atttcaatca taatattttt
    13261 aactgtcttt tcttttaatt catctaaaga ttcaacactt atttttatta ttctttcaaa
    13321 acctatttga gaaatttcta ctatatttcc accttgtatg tattttctaa gtaacataca
    13381 aaacataggc gcttttagag ggttttcttt ttgataatta cttgtaagat aaactctagg
    13441 atttgaggca cttgcactta aaaccaatct aaaattttct ttattatttc ttatattaaa
    13501 aattacctca tcatcttcag gttgatgtat cttatctatt tttccacctg taagttttga
    13561 tgataattca tcgactattg aatgtataac taatccatct aatgccattt atagcacccc
    13621 tttctttgac caaaatgagt cagtaaatta attatacaca taatatacgc tttgtgacta
    13681 gggggaattt aaacaataga gattaatatt taaattgtat aattatttat atttattaga
    13741 gaggaaactt tatttttaaa attaataatt taaagtagat taaatataaa gtaaatatag
    13801 tataattatt gtgtagagta taagctatga aagtatatga actttacaga aaaaatcaat
    13861 tttaaggttc tatagtatat aaatatgaaa gaaggttgaa aaaatgagat ttatcgattt
    13921 aagaagtgat actgttacta tgcctactga cgaaatgaga gaagctatgg caaaggctcc
    13981 tgttggagat tcaatcttta gagatgaccc tactgtaaat aaacttgaag aattagcagc
    14041 agctaaagtt ggaaaagaag atgctatttt ccttccaagt ggaacttttg ggaatcaatt
    14101 agctctattt actcattgcc taagagggca agaaataatt attggtaaag gttatcacat
    14161 tgtgactcat gaggttgggg cacctgctgt aatagctgga gttcagctta gaactataga
    14221 tgaagacgaa agtggagctc taaatcctga acttgttgaa aaggcaatta gaagagatga
    14281 catacatgaa ccacagacag ggcttatatg tgtagaaaat gcatattcag gtggaacagt
    14341 tgtgagtctt gataatttaa gagaaattaa aaaaatagct gagaaacata acttaccagt
    14401 acatttagat ggagcaagat tatttaacgc agcattaact ttgggtgtag aagctaaaga
    14461 aatagcaaag tgttgtgata gtgttatgtt ctgtgtgtca aaaggtttag ctgcaccaat
    14521 gggttctata cttgctggaa ataaagaatt tatagcaaaa gctagaagaa agcaaaaagt
    14581 tatgggtggg ggaatgagac aagtaggtat agttgcagca gctggtataa ttgccattga
    14641 aaaaatgact ttaagattga atgaagacca tgaaaatgca aaatatttag cttgtgaatt
    14701 aaataaaatt gatggcattg aagtaaacaa tgttaatcca gatataagta tggtattttt
    14761 taagatgagt gaagatatta taaaagagga tgttttaata aaagaatttt ttgaaagaaa
    14821 tataaaaatt aataaaatgg aaaatggtga atatagattt gtaactcatg tagatattac
    14881 taaggaagat ttagactatg ttttaaaaac acttaaagaa ttaataggtg tatgttaata
    14941 gatatctact agaaggatta tagctagtta ccagatgttt aattaaaaaa ttcatagaca
    15001 gtttaaataa aacatacata aaattcatat agaaatatta atatataaac atagaaagaa
    15061 ctagcttaaa aacataatat aattacccta attgtaatgg caactctccc tcacatcctc
    15121 aaattatagt tgtcattaca aaacttttcc cataaattat aagtttatgg gaattttttt
    15181 atgttataat agtttaagcg ttaatttacg aaggagcgat acttatgaaa ttttggaaac
    15241 tacatggaat aggaaatgat tttatagcaa tagatggaag attcgaccag ataaatccat
    15301 ctgattatac tgctttagca aaaaaagtat gccatagaag attttctgta ggagcggatg
    15361 gattgttagt tgtgaaagat tcaaaagttg cagatgtaga aatggtatat tataattctg
    15421 atggttcaag agcagctatg tgtggaaatg gccttagatg tttttgtaaa tttgtttatg
    15481 ataattgtat agttaaaaaa gaaagtttta cagtagatac attagatggt ataaaagata
    15541 taaaagtaaa tcttactaat agagaaataa actctataag agtcaatatg ggaaaaggaa
    15601 gttttatagc taaggacgtt cctgtactta caaataaaga acgttttgtg caagaaagta
    15661 taaaggtttt agatagggag cttaaagtta cttctatgtt gatgggggtt cctcatacag
    15721 tagttatagt agatgaatta gatgtagatg atgtttgtaa atatggaaaa gagattgaaa
    15781 ataataaaat atttcctgaa aaaacgaatg taaattttgt aaaggtagaa gataagtata
    15841 atatacatgt ttatacttgg gaaagaggtt gtggatatac tttaggttgt ggtactggta
    15901 tgactgcatc agcaatagtg tgcaatttac ttaatatggt agaaagtagt gtaaatgtaa
    15961 cgtctcaagg tggaacagtt aaaatagatg taggagattt ctcttatatg acaggtccag
    16021 cagtaaaaat atgtgaaggt attttggagg tttaattaat ggctataagt atgactggtt
    16081 ttggaagagg agaatacaag gatgataatt attacttttt agtagagtgt aaaacgataa
    16141 atcataagta ttcagatata aacatacgtc ttccaagaaa aatttcattt ttagaggata
    16201 aagttagaaa tttagtaaaa aattatgtaa aaagaggaag agtagattta tacataaagt
    16261 ttgacttatt aggcaaagaa gatgtaaact taaattttga tgagggatta gcatctcagt
    16321 atatagacat actaaaagaa ataaaaaata aatttgatat tatagatgat attagcgtta
    16381 tgaatgttgc aaagtttcct gatattgtga aaatagaaga aaaggaagaa gatgaagatt
    16441 tattgtggtc aatgttgaat caagcagttg aagatgcatt aatcaaatta agagaaatga
    16501 gaagtgaaga aggtaaaaaa ttagctgaag atattgccat gagatgtgat ttattaaaaa
    16561 atcatataga agagattgaa aaatattcaa gtagtgttgt agaagattat agagaaaaat
    16621 tgaatttgag aataagtgaa ttactggatg acccaagtat aatagatgag aatagattag
    16681 cacaagaagt tgcaatatat gcagataaaa gcagtataac agaagaaata gttagattta
    16741 aaagtcatat agggcaactt aaaaatacta tatttaaaga cgactctata ggtagaaaaa
    16801 tagatttctt aatacaagag atgaatagag aaactaacac tataggttcg aagtcatctg
    16861 atataaatat aactaattta gtagtagaag taaaaagcga attggaaaaa ataagagaac
    16921 aaatacaaaa tatagagtaa gaatacaata ttagaagggg aatgcttaaa tgtttgtgaa
    16981 aaaaggatta ttgctagttg tatcaggacc atcaggtgca ggaaaaggta ctatttgcaa
    17041 agaactactt aaagaaaatg atacaataaa actttctgta tcagctacta caagaaaacc
    17101 cagaactgga gaagttgatg gagtaaatta tttcttcata agtaaggaaa agtttgaaga
    17161 gatgatagaa aaaggtgagt ttttggagta tgctcaaatt tatgataatt tttatggaac
    17221 tccaaaagcg gcaataatgg aatgtttaga aaaaggacaa gatgtattac tggagattga
    17281 aatgcaagga gcaaaacaaa taaaagaagt ttgcccagag ggagtattta tatttgtact
    17341 accaccatca cttgaagaat taaaaaatag aattgttgga agaggtacag aaacagaagc
    17401 tgaaatagag aagagattta gctgtgcata tgaagaaatt aaaatgatta aggattatga
    17461 ttattttata tttaatgaag atgtaaaaac atctgctaaa gaaatagaag gtataatatc
    17521 atctgaaaag aataaagtta gcagatataa aaatattata atagagaaat ttaaggagga
    17581 attataatta tgttaaaacc atctatcaat gaggtactag aaaaaataga caacagatac
    17641 tatttagtgg ggactgtttc aaagagagca agaaaattaa ttgatggaga agaaccatat
    17701 gtttcaaata aaactaagga aaagcctgta tgtgtagcaa ctaaagaagt tgcaagtgga
    17761 aaaataacat atagattatt gacagaagag gaaatagaga tagaagaagc tagacatcat
    17821 gctgagcaac accaacaaat ttcagaagag gagtaaggtc atgctaaaag ataaaactgt
    17881 tgtaattggc ataagtggcg gtatagctgc atataaggct tgtgatgttg taagtaagtt
    17941 aaaaaaacta aatgcaaata tccatgttat aatgacaaaa tcagcaacag aatttgttaa
    18001 accacttaca tttcaatcgc tgagtcaaaa ttatgtagta gaagatatgt ttgaagaacc
    18061 aaaaacttgg gaagttgaac atatatcatt agcaaaaaaa gcagatttat tcttaatagt
    18121 gccagcaagt gcaaatgtta taggcaagat tgcaaatggt atagctgacg atatgttgac
    18181 aactactgtt atggcaacaa aagcacaggt tcttatagca ccagctatga atactaatat
    18241 gtatgaaaat ccaattgttc aaaaaaatat acaaacttta aagagctttg gatataaatt
    18301 tattgaaccg gaaagtggaa ggcttgcatg tggagatatt ggaacaggta aactagctaa
    18361 tccagatata atagtagagg aagttttgac taatcttact aaagagcaag atttaaaagg
    18421 tcaaagtatg atagttacag ctggtccaac tgtagagagt atagacccag taagatattt
    18481 aactaatagg tcttctggta aaatgggata ttctatagca agagaagcta tagctagagg
    18541 tgctgatgta acattaatat caggtcctac aaatctttct attccacaaa atcttaaaaa
    18601 atttgttcaa atagagtcag cacaagattt gtatgaagca gtagtatcaa atttaagtga
    18661 aaataaaata gtaattcaaa gtgcagcagt tgcagattat aaaccaaaaa cttattctga
    18721 caaaaaaatt aaaaagaaga atgatgattt atcaatagaa ctaagtagaa attatgatat
    18781 tgcttatgaa ataggaaaaa taaaagaaga taagatacta attggatttg cagctgaaac
    18841 taatgacctt atagaacatg ccaaaggaaa aataaaaaag aaaaatttag attttattgt
    18901 ggcaaatgat ttaactaaag aaggtgctgg ttttagagct gatactaata ttgtaaaaat
    18961 aatagatgta gagggaaata ttactgagta tcctaagatg aaaaaagaag aagtagccaa
    19021 tgttatttta gataaagtta aagcgttgct tgagaaataa gacctttttt aaatatgata
    19081 tttgtgtatt ttatcacagc taaagtggct gaaagctgca atttaatttt agcgagtaaa
    19141 aactcgcaaa tgtcagttaa attgtagttt tcagcattta ttttgtcata aagtataaaa
    19201 tataattaat agttaattat taaaataaag ttaatattaa taacttgaaa ttgcttataa
    19261 attctgagat tttaatatct tgtaaagtat attaaaatat gaacaatata ttaaatttaa
    19321 gtagaggttt tataatgaaa aaatatgcaa aggtaatagt tagaagtaat actatatata
    19381 cagataattt atttacatat caaattcctg tatttttatc tgatgtaata aaaattgggc
    19441 acagaatatt agtaccattt ggaaaaggta ataaaccaac agaagccttt gtatttcaat
    19501 ttacagatag cttagatgaa aaaataaaaa taaaagaaat tattgatata ttagatgaaa
    19561 atccgatatt tagaaaagaa gatttagagt tggtttattg gatgaaaaat agatatttgt
    19621 gtacttatat agaatgtata aatttgatat atccaaaggg atacaaatta aataattata
    19681 aagtagtcct attaggtgaa agtttaagtg gattaaatga tattgaattg aaggaaaaaa
    19741 tatcaacttt aagtgataaa aatagagaaa tagtagagaa agtgattgat agtaaaggta
    19801 aaattaaggt tgataagtta aaatatatac caaacttaaa tagttctttg tacacaatga
    19861 ataaaaatgg aattataaaa ttatgttggg aatataaaaa tcacaaaaat gaaaagaaag
    19921 tatgttatat atctttgagt ttagaaagtg ataagattga cgattatata gagcaaaata
    19981 aaataaatgt gggaagtaaa caaaaagaga tattaagttt tcttaaaaat aatgaaaatg
    20041 tagagataaa tgatttattg gatttattaa atgcttcaaa acaaagtata aattctctaa
    20101 gtaagaaaaa gctaataacc ctagaattta aggattatta tagagagcca aaaagtattt
    20161 acaaatccgt actaaaaagt ataaaattaa acaatgagca acaagaagca gttgatgaga
    20221 taaagtcgaa tatgtttgta gatgataaaa aaccatactt aattcatgga gtaactggaa
    20281 gtggaaaaac tgaagtttac atggaaataa tagaatttgc tctaaatcaa ggcttagaca
    20341 gtatattctt agtacctgag atagcattaa ctcctcaaac aatagatagg ctaaagagta
    20401 ggtttggaga tttagttgga gtgttccaca gtaaactatc tgaaggagaa aaacatgacg
    20461 tttacaaagc tgtcaaagca ggtaaagtaa gagtgttaat tggagctagg tcagctttat
    20521 ttgcaccttt taattcctta ggcttaataa taattgatga atgtcatgag tcatcatata
    20581 agtctgagaa gaatccaaaa tttaatgcta tagaagttgc tagatttatg gcattaaaaa
    20641 ataatataac tcttatttta ggttcagcaa cgccctcaat tgaagagtat tatagggcaa
    20701 aatctggaga atacaaactt ataaatataa aaagtagagc aaatgataag cctcttccaa
    20761 acattgaagt tgtagacatg aaagatgagt tagataaagg aaatagaagt atatttagtt
    20821 tgaaattaca aaaagaaata cgttatgcga ttgaagaaaa taatcaagta atactatttt
    20881 taaacagaag aggttatgca aattttgtat cttgtagaaa gtgtggatat gtgtttcaat
    20941 gtgaaaattg tgatatatct ttaacttatc ataagaaaag caatactgga agatgccact
    21001 attgtggata tgagaaagaa attccaaaag agtgtcctga atgtaaaagt acttatgtta
    21061 agccatttgg agttggaact caaaaaattg aagaggaatt aaaatatatt tttccagata
    21121 taaaaacttt gagaatggat aaagatacaa cttcaaaaaa aggtgcttta gatgagatac
    21181 taaataaatt taaagataaa gaagcagatg tacttattgg aacacagatg ttaagtaaag
    21241 ggctggattt tgagaatgta actttagtag gtattttgtc tgcagacatg atattaaatt
    21301 ttccagactt taaaagtttt gaaactacat ttcaattgat aactcaagtc tctggaagag
    21361 cagggagagc tgataaagag ggaaaagtag tacttcagac ttatgacact gaacactatg
    21421 ctattaagca tgctattgaa tatgattatg agggttttta tgaagatgag ataaaaataa
    21481 gaaaagcatt tggatattct ccttttaata atatgttaag tgtagttgtt agtggtgaag
    21541 atgaaagatt agtgataaaa aatataaaaa atatgcatgc atcattgatt tatttattag
    21601 aaaaaagggg tataaatgat ttaggattta tattaggtcc aaatccatgt tcaatatcaa
    21661 aaataaatca aaattataga tggcaaatac tctttaaaga tgaaaatatt gaaattaatc
    21721 tcttaaaggg tataataaaa tatatatgta taacaaaacg agatttgata tttgataaga
    21781 atatcaatgt atctatagat attaatccaa acagtgtatt ataaatttag gaggaattat
    21841 aatggcttta agacaaatag ttcaaatagg agaaccagta ttaagaaaaa aatcaaaaaa
    21901 ggttgaaaaa attgatgaaa agataataca attattagat gatatggcag aaactatgta
    21961 tgatgcagat ggtgttggac tggctgcacc tcaagttgga atattaaaaa gagtagtagt
    22021 aatagatatt ggagaagagc ttatagaact tataaatcct gaaataatag aaacatctgg
    22081 ggagcaaata gatgaggaag gatgtttaag tgttgttgga gaagcgggaa atgttagaag
    22141 accaaattat gtaaaggtca gagctttaaa tagaaatggt gaaacaatag agttagaggg
    22201 agaagaactt ttagctagag cattttgtca tgagatagac cacttagatg gaattttatt
    22261 tgttgacaaa atagaaaagt agtaggagtt gatttgatga aaatagtatt tatgggaact
    22321 cctgatatag cagttccatg tttacaaaaa ataatagatg agaagtatga aatattgggt
    22381 gttgtaactc aaccagataa accaaaagga agaggtaaaa aactaggtat gagtccagta
    22441 aaagaactgg caattgaaaa taatatacct gtatatcaac cagtaaaggc tagagataaa
    22501 gaatttatag ataaaattaa atctttaaat ccagatgtaa tagtagttgt agcttttgga
    22561 cagatacttc caaaagaaat attagagatt cctaaacttg gatgtataaa tgttcatgtt
    22621 tctttacttc caaaatatag aggtgcggca cctataaatt gggtaataat aaatggtgaa
    22681 gaaaagactg gtgttacaac tatgtatatg gatgaaggat tagatactgg agatatgata
    22741 ctaaagacag aagtaaatct tgatgaaaat ataacagcag gagaacttca tgataaaatg
    22801 atgaatatag gagcagaaac tttaaaagaa acattaagat taattgaaga aggaaatgct
    22861 ccaagagaag tacaaaatca tgaagaattt tcctatgcgc caataatgaa taaatcatta
    22921 ggtaacatag atttttcaaa aagtgccaga gaaatacaca atttagttag aggagtaaat
    22981 ccatggccaa gtgcttatac tacctataat gatgtcataa tgaaagtttg gaaaactaaa
    23041 gtgttagatg aaaaaagcac aaaagatgta ggtacaataa ttgatgtaag caaagatggg
    23101 ataaaagtaa gtactataga taatgtactt ttaattgaag aaattcagat gccgaataaa
    23161 aagagaatgt tggttggtga atatataaaa gggaatacta tagaaactgg tttagtattg
    23221 ggttaggtga aaagtatgac agatataaag aagtatttaa caagtgttgg gattttgatt
    23281 ttaatattag gaattttgat aggagttgtt aattttttta taataagttt aactcaaata
    23341 ttctccttga tgataaatat tataataata tcactaatac ttataatact atgttcaaca
    23401 gtagttactt atagagtaat taaaggaaag tatgttaact caaatataat gaaaataaat
    23461 tttaatattg tcagtggatt atttcctatt atatctttca ttgcatcatc atttggtatg
    23521 tcaaaaagtg atattagaag aatatatata aagttaaata atgaatatat atacagtaat
    23581 aaatataatt ttaatccaga ggatataata attttaatac ctcattgtat tcaagagaat
    23641 agttgtaaat taaaagttac taatgatata gataattgta aagaatgtgg aagatgcaac
    23701 ataggagaat taattaagct aaaggaaaaa actaatgtaa aaatatttgt agctacagga
    23761 ggaactctag ctagaaaaat tataatggac actaaaccta aagctgttgt agctgttgca
    23821 tgtgaaagag atttgacatc tggaatacaa gatattaaga aaattcctgt tttaggtgta
    23881 tttaacaaaa gacctaatgg accttgtgta aatactaatg tagacatgat ggatattgaa
    23941 aaagctatag gctttttaac aggaaaaaat atattagttt gtaattaaac tgtttttaag
    24001 ttaagtgaat attaagagga tgtataataa aaatatattt taggagagtg ttatttatgt
    24061 accctgtata tggcggtttt tggggatttg acccaacaat ggttgtatta attccagcta
    24121 tattacttac tatatatgca caatttaaag tgagttcaac aacaaataag tatttaagag
    24181 taaatacacg tcgaggatat actggagagc aaacagcaag aagagtactt gattcaaatg
    24241 gtttgtatga tgtaaagata gaaatggtta gagggcatct aagtgaccat tatgacccaa
    24301 gaagaaaggc tgtaaggttg tcagaagatg tatattatgg aacatctata acttctgttg
    24361 cagtagctgc acatgaatgt ggtcatgcca tacaacatgc taaaggttat gcacctcttc
    24421 aaataagaag tagtttagta ccagtagtga attttgcttc aagtatatct tggtttttaa
    24481 tatttttagg atttattatg gctggtccat ttttaaaaat tggtatacta ttgttttcgg
    24541 cttcagtgtt attccaaata ataacattgc ctgtagaatt taatgcttca agtagagcaa
    24601 tcgttcaact tgggaattta ggtattatag atgaaagtga gtcaagacaa agtagaagag
    24661 tactttcagc agcagcttta acatatgttg cagcagcatt ggtttcaata cttcaattac
    24721 ttagattgtt actaatagct caaagaagaa atgactaaaa gtaaagtggg ttgtttagaa
    24781 acaacccttt tttatgttta ataaaaggga gaaatttata tggatgcaag agaagttgga
    24841 tttaaagtat tatgtgatat agaaaaaaat gacaattatt caaatattgc aataaataag
    24901 cattttaaaa atttagaaat aagtgatatg gatagaggac ttgcaacaga gttaatttat
    24961 ggagtaatag aaaataaata ttatttagat tatataatca ataaactttc aaaaataaag
    25021 gtaaaaaaaa tgtcaactta tgttaaaatc tttttaagaa tgggaacata tcagatttta
    25081 tttttagata gtatatcaga ttatgcagca gttaatgaaa ctgtaaaatt atctaagaaa
    25141 tatgataaaa aatcttctgg tttcataaat gctattctta gaaatgaaat aagagctaaa
    25201 gagactataa tggacataac tgaggaagat agtgtaaaat acttatctat aaagtattcc
    25261 tataactctt ggattataaa aaactggata gataaatttg gacaagaatt tgctgaagat
    25321 ttgttggaag caaataatga aaagcctagt atctatataa gaacgaatac acttaaaata
    25381 agtagagaag aacttattga aaaattaagt gaaatgggta ttgtatgttt aaaagttcct
    25441 atggtagaag aagctattaa agtcgaaaaa atgaaaaata tagaaaaaaa tgaattattt
    25501 aaggctggtt tatttacaat tcaagatata agttctatga tagtagggaa agtaattaat
    25561 ccaaaagaag gctcatttgt tttggatgta tgtagtgctc ctggagggaa atctactcac
    25621 ttagctactt taatgaacaa tactggtcaa gtagtagcta gagatatttt tgagcataaa
    25681 ctaaagctta taaactctac tgtaaataga ttggggctta aaaatgtgtt tgtagaaaga
    25741 tttgatgctt ctgagattga tgaaaatagt ataagtaaat ttgattatgt tttagcagat
    25801 gttccttgct ctggttttgg tataataaga cgtaaaccag aaattaagta taagaaagaa
    25861 gaagagttaa aagacatcac ttctatacaa aagaaaattt tagaaaatgc atctaaatat
    25921 gtcaaagttg gtggaacttt agtatatagt acatgtacaa ttcaagatgt ggagaatata
    25981 aatatagtta cttcttttgt tgaagaaaat cacaactttg aatttgttcc aattgataca
    26041 gtaaatgtag acttagacaa tcaagataaa ggatatttaa agatatatcc taatattcat
    26101 ggtatagatg gttttttcat agcaaaatta aagagaataa ggtagtgaga gaatggaaaa
    26161 gaaaaagata gtattaaaaa attttactga agatgaactt aaagaattta tgaaaactat
    26221 agatgaaaag ccctttagag gaagtcaaat attctcttgg atatataagg gtgcaaaaac
    26281 ttttgatgat atgaacaata taccaaaaag tttgagaaat aaattagaag aggtttcatg
    26341 tatagggcat atagatatag aattaaagtt agaatctaag gtagataata caaaaaaata
    26401 tttgttttta ttagatgatg gaaatataat agaaactgtg atgatggatt atgatagtag
    26461 agttactgtc tgtgtttcta accaagtagg ttgtaggatg ggatgtaatt tttgtgcatc
    26521 tacaatggat ggattaatta gaaacttaga gccatgggaa attttagacc aagttattaa
    26581 aattcaagaa gatactggta agagggtatc aaatcttgta ttgatgggga gtggtgagcc
    26641 acttgataac tttgaaaata caaaacaatt tttaaaaata attaacgaaa aaaatggtct
    26701 taatataggg tataggcata taactctttc aacttgtgga atagtaccta aaatgtatga
    26761 gttggcagac ttagaaatag ctataaattt agctttatca cttcattcac catatgatga
    26821 agaacgaaga aaaattatgc ctgtagcaaa tgcttattct attgaagaaa tattgaatgc
    26881 atgtagatat tatataaaaa agacaaatag aagggttaca tttgaatatt ctcttataaa
    26941 aggggtaaat gactcagaaa aagaagcaaa ggcattagct aagctcctaa aaggtatgct
    27001 atgtcatgta aatttaattc caataaataa agttgaagaa agagaatatg aaaagccaga
    27061 taaagcattt atttataaat ttagggatag tttggagaag aacaatatac ctgccacagt
    27121 taggatgtct atgggttctg atattagtgg agcttgtggg caattgagaa gaaaatacaa
    27181 gtaatttgta aggaggaagt cgtatggttt atagttgtgc ctcccatata ggaaaaataa
    27241 gaaaaaacaa tgaagactat tgtgagggag aagttataga tacagaacat ggtccaatag
    27301 gaatatttgc catagctgat ggaatgggtg gacataaaaa aggtgaagtc gccagtaaat
    27361 tagcagtaga aaatataatt gattttctaa aggaaaactt attacaacat gataatgtaa
    27421 aaatagacta tatagatgat atattaaaac aggcttataa taatgtaaac tcaattgtgc
    27481 acaaaaaatc tatggaagat atagaatttg aaggaatggg aactacatta gttacggcta
    27541 tagtgtataa taatgtctta tatgtggcaa atgttggaga tagcagatgt tatttgttaa
    27601 cagatgaaaa atttgataaa ataactatag accattccgt ggttgaagaa cttatgatgg
    27661 cccatgtcat tacagaagaa gaggcaagaa gacatccaca gaggaataga ataactagag
    27721 caattggaac agatgatatg gttgttgtag atatttttaa gaaagaaatc aaaaaaagtg
    27781 atataatact tcttgcgaca gatggattaa ctgggtttat tgatgatgaa gatataagag
    27841 atttaatatt agattatgaa tatgagagaa ctagtaatat aagtgaagaa ttgataagta
    27901 tggccaatga tgtttctggt aaagataatg tttcggttat agtgataaaa gtataattag
    27961 gacggtgata ttgtgggaga tacaatttta ggaaatcgat atgaaatcat caggaagatt
    28021 ggtgatggag gaatggcatt tgtatatgag gctaaagaca gattattaaa caggactgtt
    28081 gcacttaaag tacttagacc tgaatttgta gatgatgatg aatttctaac aaagtttaaa
    28141 agagaggcag aagcagtagc aagtctttca catccaaata tagtaaatgt ttatgatgtt
    28201 ggtgaagatg gaaaagttca ctacattgta atggagtttg tagatgggaa gaatctaaaa
    28261 gagattatac aagatgaagg tatattagat gaatatactg cattagatat aacaaaacaa
    28321 atagctatgg cacttagtgc agcgcacaaa aaggggatta tacatagaga tataaaacct
    28381 cataatattc tcatatcaaa tgaaggaaga gtagtaaaag tggctgattt tggtatagcc
    28441 aaagctgttt caaattcaac aatgactaat attggtagta taataggttc agtgcattat
    28501 ttttcaccag agcaagctaa aggtaaattt gtaactaata atgctgacct gtattctcta
    28561 ggtatagttt tatatgaaat gttgatagga aaagttccat ttaggggaga tagtccaatt
    28621 tctatagcac ttcaacacat taatgatgat atagacttta catcagaaga aaaagttagg
    28681 attcctcaaa gtgtaagaac tacaataaaa aagcttacag aaaaatctag tgcagataga
    28741 tatcaaactg ctgaagaatt gatagaagat atagagtata tagaaaaaaa tattgattta
    28801 gattttataa aagaatatga tgattttgca actaagaaaa ttgatgaaaa agaaattaat
    28861 aaagttgtaa atccaacact tgcaaaacca gctcctgaaa aagtggttaa gcctgttgaa
    28921 gtagctgatt tggatgatga tgaagattac tatgatgatt tttatgaaga agatgatgat
    28981 gaagatgaag aagaggaaga gataatgaga gctaaaaaga atcagagacc taagagtact
    29041 ccaagtaaga gaactaagaa aaagaagaaa aaacaggaaa gcccaaaatc tagaagaaga
    29101 ttaaaagtaa ttgcggctgt tttgatactg atattgtgtg ctcaagtatt cttagcatat
    29161 aagtttttat ttgctggagg gtttggcaat aaatctttga ctgttcctaa tcttgtaaat
    29221 atgacattag aagaagctca aagtgctgtt gaaaaagaag gattatacct gagtgtaaaa
    29281 agtgaagagt ataatagtga agttgatgaa aattgtataa tatcacagac acctgaaggt
    29341 ggaagtacaa atgtcaaaaa gggtgatact ataaatgttg tcgtaagtaa aggttcaagt
    29401 caggcatctg ttcctaatgt agtgggactt actttgagta atgcaaaaca acttattgaa
    29461 gaaaataact taaaagtggg aactgttaag tatgaataca gctcaatata taaagaaggt
    29521 acagttttaa gtcagagtcc aggtgctggc tcatcaagag ctcaggaagg tgatgaggta
    29581 aatctatatg taagcaaagg ttcagaaaaa tcaaatacac agactccaac agtaccagat
    29641 aaaaaaccaa caacaccaga gaataataca ccaacagaac ctggttcaaa ctcaggaaac
    29701 agtggtggaa attcaggagg ctcaaattca ggaaatagtg gtggaaactc aggcaataat
    29761 ggaagtaact caggaggttc aaactcagga aatagtggtg gaaactcagg aggctcaaac
    29821 tcaggagata atggaggaag ttcaggaggc tcaaactcag gagacaatgg aggaagttca
    29881 ggaggttcaa actcaggaga caatggagat agttcaggag gctcaaactc aggagataat
    29941 ggaggaactt catcaggaac tggcgcaaac attggtaaaa cagaataaat attaataaaa
    30001 taagagctgt ccttttatag aaaatatttt ctttaaaggg cagtttgttt tgtataaaat
    30061 actgaattgg ttataatata aggagaaaag tatttatgaa gaagaaaaca gttttaatta
    30121 caggaggagc tagagggatt ggtaaagcta tgtctaaagc ttttgcaaaa gaaggttaca
    30181 atgtattagt aaattttaat aagtcagaaa atgaagctaa agagttgtac actatattaa
    30241 atgaaaaaaa tttttctgta aaacttttta aggctaatat ttcaaataga gaagatgtag
    30301 aggatatggt agattattgt ataaaagaat ttggaggatt ggatgtactt gtaaacaatg
    30361 ctggtgtaag tcaagataag ttgtttacag atataactga tgaagattgg gataatatga
    30421 tgaatattaa tttaaaagga agcttttatt gttcacaagt tgctttaaaa tacatgatat
    30481 cagaaaaaaa aggaaatata attaatatat cttcaatctg gggtatatca ggtgcgtctt
    30541 gtgaagtgca ttattctata acaaaagcag gtataatagg tatgacaaag gcattagcta
    30601 aagaagtagg accatcaaat attagagtaa atagcatagc tccaggtgtc attaatactg
    30661 acatgttatc tggatataat gaggaagata tagacgctct tgtggaagaa acccctctaa
    30721 tgaggctagg aactccagaa gatatagcaa attgtgctat tttcttggca tcagacaaat
    30781 caaattttat cactggtcag gtaattagtc caaacggagg atttgttatt taaaaaaaat
    30841 gtggtattat ataaaaatag gaaagaataa cacgatatac tactcataaa cagtatgtgt
    30901 ttatataaat tttataaagg gaggcatttg atgctagaag gaaaaattat aaaggggatt
    30961 agtggatttt attatgtaga tacttataat ggtatttatg aatgtaaggc tagagggata
    31021 tttagaaagc agaaaataac acctttggtt ggtgatagag tcaaaataag cattgttgat
    31081 gaagatgaaa aaaagggtat cttagaagaa atcgacagta gagatacaga acttataaga
    31141 cctcctattg caaatgtaga taaagcatta atagtattcg caataaaaaa tcctaaacca
    31201 aacttatctc ttttagatag atttatagtt ttggcagaaa aagaaaattt agaaactgta
    31261 ataatcctta caaaagcaga tttagatgat aatgatacat tagaaactgt taaaaatata
    31321 tatgaactta gtggttataa agtcattcct gtaagcaata ttactaagtt aaatatagat
    31381 aaagtaaaag aagaattaaa agaaaatgta gtagtgtttg caggaccatc tggagttgga
    31441 aaatcaagct tattaaatga aatagatgaa aattttaaac ttcaaacagg agttgtaagt
    31501 gataaaataa aaaggggaaa acatactact cgtcacgctg aacttttaaa attagaattt
    31561 ggtggaatgg ttgcagatac accaggtttt agttcccttg cacttgaaga tatagaggaa
    31621 gttgaactta aagattattt tatagaattt gataaattta atgattgtaa gtttggttca
    31681 aagtgtattc atgaaaatga acctaattgt gctataaaag aagctgttac aaatggagaa
    31741 atatctaaag aaagatatga tagttatata cagctgctac atgaaataag acaaaataat
    31801 agtaggaggt attagaaaca tgattaaatt agcaccatca atattatcag cagattttgc
    31861 aaaattatta gaagatgtaa gaaaagtaga aagtgcagga tgtgaatatc ttcatatcga
    31921 tgtaatggat ggacactttg taccaaacat tacattagga ccacttgtag tcaaaagttt
    31981 aaagaaagaa aatataaata tggtatttga tgctcatctt atgatagaaa atccagacca
    32041 gtatattgaa gaatttgtaa aagcaggatg tgatataata actgttcacc aggaggcttg
    32101 tgttcatcta catagaacta tacaaaatat aaaatcacat ggaataaaag ctggtgtagt
    32161 acttaatcca gctacaccag tagatacgat taaacatgta ctatctgatt tagatatggt
    32221 attattaatg tctgttaacc ctggatttgg aggacaatca tttattcctt gtgttttaga
    32281 taagattaaa gagttaaaag ctattataga tagtcaagga ttaaacattg atatagaagt
    32341 tgatggtggt ataagcccta aaaacgtagc cgaagttgta caagcaggag ctaatgtaat
    32401 agtagcaggt tctgcaatat ttggaagtga tgacattcaa gaaacagtta atttatttag
    32461 aaagaatgct tctcttgaag aattagttta aatttaataa tgaaaatctg tattgtttta
    32521 aatggcgaaa ttgaagatta cgaagttaca agagatatta taattaagga atgttatgac
    32581 tgcataatat gtgcagatgg aggggcaaat cacacttata aaatggaaat aatgccagat
    32641 tatatattag gagatttaga ttcagtagaa gaagaaaaga taaacttcta taaaaataaa
    32701 ggtgttaaat ttgaaaaatt tccatctaaa aaagatgaga ctgatacaga gctttgtctt
    32761 tttttagcta agacacttaa agcaaatcat attgattttt ttggtgcttt agggggaagg
    32821 attgaccaca ctcttgcaaa tataaagcta ttatattatt taaaagaaga tggtatatat
    32881 tctaggatac tttctgataa agaagagatg tatatagttg aaaatgagga aatttcattg
    32941 tatggaaacc caggggacac tatttctgta atagctataa atggtgatgc taagggtgta
    33001 acacttactg gcttagaata ccctctcgat aattattata tgaaatactc tgtacctatt
    33061 ggaatatcaa atgttatgtt gagtaattct tgtaagatta aagtagagca aggctgtgta
    33121 cttgtagtta gaaatttata aaatttgatt ttgacaattg gttttatatt gtagagcaga
    33181 tacaatcaag taaaatatat ttatgattgt atctgctttt tagtatataa ttataaatat
    33241 ataataaact ttgacataag ggggattttt tgtggtagat agtattattt ttgatttaga
    33301 tggaacattg tgggattcaa ctgaagaagt atgcaaagtc tggcaggatg ttttaagtga
    33361 gcataaggaa atagaattaa gtgtcacaaa agatttattt agaagtctta tggggctttc
    33421 ttttgaggaa atagcaaatc gtttatttcc aaatttaagt gaagaagaaa gaatgaattt
    33481 attgaatgaa tgttctttga aagaatgtga ctatttatct gaaaatggag ctaaattatt
    33541 tgatgatata gaagatactc ttaaattact atctaaaaaa tataaattat ttatagtaag
    33601 taattgtcaa gctggatata tagaatcttt tttaaaagca cataaactag aacagtattt
    33661 tatagatttt gaatgtcctg gtaatactgg tttgcataaa ggagaaaata ataaacttat
    33721 aattgaaaga aataaattaa taaatccaat ctatgttggt gatacgcaag gtgatgcaaa
    33781 tagtgctaag tttgtaggta taccttttat atatgccaaa tatgggtttg ggaatgtaga
    33841 tgtatatgat tattttatag attcatttaa agatttgtta gaacatgata tattaaaata
    33901 agatgagttt aaaaattata ttaaaataat aaaaaagtta tggggggatt taaaaatgga
    33961 attacaagat actattttta aaagacaaag tgtaagaaag tttaaaaatc aagatgtatc
    34021 agatgaagat attttaaaaa tgataaaagc agcaggtgca gcaccatcag gcaagaatat
    34081 tcaaaactgg cattttgttg ttataaaacg ccgtgattta atggaaaaga tagcagatgt
    34141 gataactaaa aagcaacaag aaatacttgt agaaatggat aaagtatcag tggataaggc
    34201 taatagattt agaaaatttg ttaaaaattt tacactattt tacttaaaag ctccagtttt
    34261 agtattagtt tttacaaagg tatataatcc atcaggatat tatgaattag agcttataga
    34321 tgcacctaag gagactatag ataaattatt tataagaaat ccaggtatgc agagtctagg
    34381 tgctgcaatt gaaaacttta cactatcagc aattgaactt ggatatggtt cttgttggtt
    34441 gacaagtcaa aactatgcag cagatgagat agaagctgtt ttagaagcag aaactggttt
    34501 tgaaaaggga gagtattttt taggtgcaat gttagctctt ggagtgccag aggacaattt
    34561 gaagagtcca tctaaaaaac cagtagaaga aatatgtaca tttataaaat aaaagtcaag
    34621 gacattttaa aatcgaagta aattaaaaac aaaataaatt aaaaataaat atttaaaaaa
    34681 ttaaaaagta ggttttattg atattaattt tatatttcaa atcattaatt taattaatac
    34741 aataagaact actttttttt gtatattaat tttaaatata tttaaatctt tagaaaatct
    34801 ttagaaaagt atatgtaata tcttaagttt ttaattttaa tattaagtat ataaaacaaa
    34861 atgaaaaaat attaaattta aactagaagg aggtttctaa gatggacatt aaatacaaat
    34921 aaccagaaca aaagatataa atatatatat aaatggaaaa acttctatca tattgtataa
    34981 tcatatatgt aaagaaaatt tataaattaa tataaaggag attagaatga attcatataa
    35041 tattttggtg gtagaagatg aaaaggagat agcagatgct atagaaatat atctattaaa
    35101 tcaaggatat aatgttttta agggatacaa tggattagaa ggtcttaagg ttatagaaaa
    35161 ccaagaaata catttagcta tagttgatat aatgatgcct aaaatggatg gtataacact
    35221 tacaatgaag cttagagaaa actataattt tccagtaata atgctatctg caaaatcaga
    35281 agaagttgac aaaataatgg ggcttaatat aggagcagac gattatgtaa caaaaccttt
    35341 taagccatta gaactattgg ctagagttaa ttcacaactt agaaggtaca ctaagtatct
    35401 aaacatggtt ggaaataaag agcaaaaggt agaaagtgac gatagtgtgt ttgcaatagg
    35461 cggattagaa ttaaatgaaa atacaaaaga agttagtatt gatggaaagt atataaaaac
    35521 aactcctata gaatttaaaa tactaagttt acttatgaga aatgcaggaa gagttttttc
    35581 tgctgatgaa atatatgaaa gagtttggaa tgagaatgct gtaaatacag atacagttat
    35641 ggtacatgtg aggaatataa gggaaaagat agaaattgac ccaaaaaatc ctaaatattt
    35701 aaaggtggtg tggggtgttg gatacaaaat tgaaaaaatt taaagataaa tatatagtta
    35761 ctattatagt ttttatactt atgttatcag catcattagg tatgattagt caatattcta
    35821 ctatacaagc aagtgctaaa ggtggagcag agaatccatt tgaacaggaa agctttgtag
    35881 atagtatata taaaggaagt tatgttttat atcataatat gaaggaagaa caagaaggta
    35941 agatgataca accatcaaag ctatttatca atgaagaaac tatgaactat ataaaaaatg
    36001 taagtaaaga aaaagatact tatgatggtt cagaaactta ttatacagat gatagtatta
    36061 atgcagattt taatgaaaac tttgaaaatt gggagcattt cattaaagcg tcaggaaaga
    36121 atttaaagta ctatttagtt gataaagaaa gcaattttga attttttaat gataataaag
    36181 aattaggagt acttgataca acaaaaaatg atgcttcaaa agaaaattct aatgaaaatc
    36241 agtcaaaaaa tacagaaatg aatgatgagg aaagacaaaa gcagattaca gatataaaag
    36301 aaaactatag attctacgtt gtaatgaatt ttgataaaga tggaaaagta aatattgtgg
    36361 attcttatgg agtaaatcaa aaaattatta atcaaatgtt gttatctaaa gcaagagaag
    36421 atttaatgga tacaagttta ggaggaaatt caatttcata taagttatct ccaataaaaa
    36481 atataacatt agtatatgct gtacctaaag taataagtaa aggtgatagt gtagagtatg
    36541 gttatggaaa tacttatttt gatgatggta taatttcata tataagcaat gtagaaaata
    36601 attcttatta taagatttct ggaatgattt taaaaattat attagctata gttgttattg
    36661 tagctttagc attcccatat agaaaatcta aagagttgct aggatttgat acattatcaa
    36721 gaataccgtt agagatactc tttattgctc ttgctattat acaggggctt gtagatttat
    36781 atcctactgt tataataagt aataccttga gtgggaatta tgtagagtac cttataaaca
    36841 atgacataag tgctaaaatg gctagtatat tggtaagttt aatgaattta acttattggt
    36901 ttgtaatatt ctcaataata tttgtattta taatactttt aaaacacatt ttaaatgttg
    36961 gaataagaga gtattttaca aaacatactg tgacaggaat gttttttgta tggttaaaaa
    37021 aagcaggaaa agcagttatt aatcaaatta gaacaataaa tctgaaagaa aagccaaata
    37081 aaactatagt tacaattttg ggaataaatt tattgattat agttataatg tgtagtatgt
    37141 ggttctttgg aataatactc gcattaatat attcagtagt attatttaag atactgtcag
    37201 attactctaa aaagacaata tatgaatata atcaactttt agatgttaca aagaaaattt
    37261 cagatggaaa cttagatgta aatatggaaa aagatttagg attttttaat cctataaaag
    37321 atgaattagg aaatattcaa actggattta aaaaagctgt tgacgaagag gttaagagtc
    37381 aaaagatgaa aacagagctt atatcaaatg tatcacatga cttaaaaaca cctctaacat
    37441 ctataatcac ttatatagat ttattaaaag atgaaagtat aacagatgaa aatcgtaagt
    37501 tgtatattga tacattagac agaaagtcac aaagattgca acatctaata gaagatttgt
    37561 ttgaagtaag caaggcaaat agtggagatg tttatttgaa tatagtaaat gtggatattg
    37621 tatctctaat gaagcagaca cttttagaat tagatgacaa acttgcagat tcatctctaa
    37681 tagtaaagaa taacttttct aatgaaaaaa taatattacc attagatagt cagcgtacat
    37741 ttagagtatt tgaaaatctt ataataaata taagcaaata tgcaatgcca aattcaagag
    37801 tgtatattga tattttagaa acagataata aagtaaatat tatgcttaaa aatatgtctg
    37861 catctgagat tgattttagt gtagatgata taatggagag atttgtaaga ggagataaat
    37921 cacgtaacac agaaggttct ggtcttggtt tagcgatagc aaaaagtttt atagaattac
    37981 agggtggtaa gatgaatata agtgtggatg gtgatttatt caaagttaca attactttta
    38041 acaaaaatta gattagactt ctttaataat tataaagtct atagaataaa agaatctgga
    38101 aatatttatg tgtattcttt aaataaaaat ttat
//
LOCUS       DAMKMI010000334        17228 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.guided.089, whole genome shotgun sequence.
ACCESSION   DAMKMI010000334 DAMKMI010000000
VERSION     DAMKMI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.guided.089"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            complement(284..808)
                     /locus_tag="PC429_003891"
     CDS             complement(284..808)
                     /locus_tag="PC429_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF3849284.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(873..1625)
                     /locus_tag="PC429_003892"
     CDS             complement(873..1625)
                     /locus_tag="PC429_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF3849285.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1656..2954)
                     /locus_tag="PC429_003893"
     CDS             complement(1656..2954)
                     /locus_tag="PC429_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF3849286.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2971..5235)
                     /locus_tag="PC429_003894"
     CDS             complement(2971..5235)
                     /locus_tag="PC429_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF3849287.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5490..6308)
                     /locus_tag="PC429_003895"
     CDS             complement(5490..6308)
                     /locus_tag="PC429_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF3849288.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6301..6999)
                     /gene="cbiQ"
                     /locus_tag="PC429_003896"
     CDS             complement(6301..6999)
                     /gene="cbiQ"
                     /locus_tag="PC429_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF3849289.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(7002..7280)
                     /locus_tag="PC429_003897"
     CDS             complement(7002..7280)
                     /locus_tag="PC429_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF3849290.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7270..8022)
                     /locus_tag="PC429_003898"
     CDS             complement(7270..8022)
                     /locus_tag="PC429_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF3849291.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8204..8396)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8528..10300)
                     /locus_tag="PC429_003899"
     CDS             complement(8528..10300)
                     /locus_tag="PC429_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF3849292.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10314..11243)
                     /locus_tag="PC429_003900"
     CDS             complement(10314..11243)
                     /locus_tag="PC429_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF3849293.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11472..11660
                     /locus_tag="PC429_003901"
     CDS             11472..11660
                     /locus_tag="PC429_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3849294.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11653..12354
                     /locus_tag="PC429_003902"
     CDS             11653..12354
                     /locus_tag="PC429_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3849295.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12356..13279
                     /locus_tag="PC429_003903"
     CDS             12356..13279
                     /locus_tag="PC429_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3849296.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13436..14359
                     /locus_tag="PC429_003904"
     CDS             13436..14359
                     /locus_tag="PC429_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3849297.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14352..15089
                     /locus_tag="PC429_003905"
     CDS             14352..15089
                     /locus_tag="PC429_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3849298.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            15100..15873
                     /locus_tag="PC429_003906"
     CDS             15100..15873
                     /locus_tag="PC429_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3849299.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAWGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16414..17022)
                     /locus_tag="PC429_003907"
     CDS             complement(16414..17022)
                     /locus_tag="PC429_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF3849300.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5575 a         2975 c         2158 g         6520 t
ORIGIN      
        1 aagtttaaaa aatactgaaa taagtgtttt ccacatattt tatatattta tttaatacat
       61 tattatttaa aaataaatat ataaacaatc ataataaaat ttttttaata aaactttatt
      121 ttttagttaa aatcatacaa tatatagatt tccacaatac aaataaatta aaaagtgaaa
      181 tgtatttgtc taaaaacata ataacatttc actttatcct catttttaag taatatatat
      241 ttttaatttt tatttataac taaatctagt ttttatataa attttagttt ttttctacat
      301 ctgagtaatc tattccaaaa gtttctacag taacagattt cataatttgt ggagtttgtg
      361 gcttatcagc tgaatctgtt ctaactgatg ctatttcatc aactgtatcc ataccttcta
      421 tcactctacc aaaacctgca tactgaccat ctaagtgtgg tgagttttta tgcattatga
      481 aaaattgaga accagctgaa ttaggtgcca tagttctagc cattgatatg actcctcttt
      541 catgttttaa attgttagta aatccattac cactaaattc accttttatt gagtgacctg
      601 gaccacccat tcctgtacca tttgggcatc ctccttgtat cataaatcct ggtattactc
      661 tatgaaatat tattccattg taatatcctt catttactaa tgatacaaag tttttaactg
      721 tatttggagc tatatttgga tataactcaa ttttgatttc ttttccattt tccatttcta
      781 tagttactat aggattttta ttttccataa tttattacct tccttttcat tagttaaatt
      841 acataattac atttattata ttatactcta aattaaagtc tatcaagtat attttttcct
      901 atttcatatc cttcttgata aatagtttta caaaactcat catcaaagtt aagcgtgtct
      961 ttaggagcaa attgagtatt aggtctaact actattattt tatcttcatc atatactttt
     1021 ttaatatctt ctggcaactc ataattatat tttgtggata ttaaaataat tttatccaca
     1081 ttatccccaa ttaatggttc aattaatgca ctcctaatta aacctccatc taatttgaaa
     1141 ccatcataaa ttccttcttg cacatctttc acaaattgtt ctttaaatcc aagagttgcg
     1201 tttggatttg atggtaacaa cccactgtat gaaatgctga taatggcttc attacgaggc
     1261 atttttgata catcaacaat cttttctgat actactttat tatcaacttc aacataatta
     1321 acataaaagt tcatttcttc tatattacta tcatctagtt cccttaggtt gtctatagca
     1381 ggagaattat ccacagtatt atttactatt tttacatttc cattctggat atcttttata
     1441 tttgtccaca ttttctccaa gttatcaaca ttccctgtgt acaaataata tccatttatt
     1501 gctcctattg atgtccctga aatcgaataa aatttattta ttcctccatc atacaaggct
     1561 ttaactactc ctgcttgata agcccctttt gctcctccac cttctaaaca taatcctaat
     1621 ttcatagtct catctccaaa ttgtttatct tatatttata tttcaaaaaa cttactaaat
     1681 gttttatata tactatagtg gtcctcaaca tttcctaatt ctcttataga atgcattgct
     1741 aaaattggac ttcccacatc cacagatggt atatctatat gagtagaaga tattggacct
     1801 atagtacttc ctcctctttc atctgaccta ttaacaaatt cttgatactc aacattagct
     1861 tctctacata tatttttata aatagctata gaatatccat cactagtata cgcctgacta
     1921 gcatttatct ttataaccgg accttttccc attattggtt tattagttgg gtcatgcttt
     1981 tctggaatgt ttggatgaat agcatgagct aaatctgatg atatcataaa agatgaataa
     2041 atagactcaa agaatccctc tctatcctta cccattgata tacaaattct ctccaatata
     2101 tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc taccaacttc ttcattgtca
     2161 aatacagcca ttacatttac accatgttga cctttagtat taacaagagc attcaaacta
     2221 gcatgtacca ttgcaagatt atcttgtctc ccaatagata taaattcatc attagcccct
     2281 attaaacttc ctttttcaaa ttcatataaa aataaatcaa aatctataat atcgtccata
     2341 tttacatcta agttagatgc tatttgcttt ataagaaaat tatctttttc taatgtatca
     2401 tttaacatcc caactaaagg taacatatct ttttgcttat tatatgaatg accatcattt
     2461 atacttctat ttaaatgtat tgctagattt ggtattatac aaattggctt tttaaagtct
     2521 ataagccttt catttggttt taatatacta tcacctttta aaactactct accagctatc
     2581 ccaagaggtc tatccaacca tgtacttaat attgctcctc catagcattc tgtatttaac
     2641 ttcaaataag atttttcagc ttctatttct gcatttggtt taatactaaa tgtaggagaa
     2701 tctgaatgag agcctattat tctaaatcca tctctttcta tatcttctga atttactaca
     2761 aaagctacta tagctgataa attcttagta acataatact ttccaccaac tcttagattc
     2821 catctttgat tcaaaactaa ttcttcaaat ccatttttat ctaagatttc cttagtactc
     2881 ttgactgcat gaaaagacga cggactatca tatatgaagt ctattaagtt tttagcaaag
     2941 tttatactat ccattcctca tttacctcca ttataaaata tttagtgttg tatattcttt
     3001 atttattttt ttaattccct gttggtcaaa agctatagta acatctgtac caattataga
     3061 aaccactgtc ccaaccccaa atttaggatg atgtacctta gaaccaagtt ttatatcatc
     3121 tggattagtt tctttattag aatcttttat tgtagcatta actttatctg caactttaga
     3181 tttgcttata gtactcatat actttttagt atatttatct aacatattat aacttgcttt
     3241 agaatagctt agttcttttt ttactttgta caatctttct atacattctt ctggcagttc
     3301 ttccataaat cttgaagcta ttgcaacatt tgttttacca tataatgttc tcttctctgt
     3361 caatgacata tatagctctt ctttagctct agttatacca acataacaaa gtcttctctc
     3421 ttcttctatt tgtgaatcac tcatagattt tatagctctt gatataggaa ataacccttc
     3481 ttccatacct gttaaaaaca caacagggaa ctctaaacct ttagatgtat gaattgtcat
     3541 aagcgaaacc ctatcattgt cttcttcatc atttgattca gatgtaagtg ctatacttgt
     3601 taggaatgtc tctaagtctt tattttcata agtatcatca ttactacttt caaactccaa
     3661 agctatagaa ataaattctc tcaaattatc aattctgtct tgagcttctt cacctttgcc
     3721 tgttaaatct ttttcatttt tatttcttat ctctacaagc tcgtccatat atcctgtagt
     3781 gtccaagact ttttctataa gtttgctaac aggataaact tcttttatag ttctaagagt
     3841 ccctataata tcaacaaatc cacttatact tgcccttgcc ttagtagata tatctgaatt
     3901 tgtttctata tcaattaata ctgagtatat actttcctgt tttaagttag ctctatcttc
     3961 aattttttct atggttctaa gccctatacc tcttctagga acatttataa tcctttttat
     4021 agatatatca tcttgtggat tttgaataac tcttaaatac gccaacaaat cctttatttc
     4081 ttttctctca tagaattttg taccaccata tatattgtat ggaatttgac ttctatttaa
     4141 agcgtcctct acaggtcttg cctgtgcatt tgctctataa agtactgcaa aatccttata
     4201 aggtctattt tcttttctag ctatttgtgc aattgaatca gcaatgaaat cagcctcttc
     4261 tatttcacta ccagttaatt gtatctttat aagttctcct tctttttttt cactccaaag
     4321 cttttttctt tttctttcta tattattaga tataacttta ttagctgcat ctaatatcac
     4381 ttgtgtagac ctataatttt gttccaactt tacaacatgt acattgtcat aatctttctc
     4441 aaattcaagt atgtttctta tatctgcacc tctccatcca taaatacttt ggtcatcatc
     4501 tccaacaaca caaatatttt gatgttctct tgctaataat tttatcaatt catattgagc
     4561 tttacttgta tcttggtact catctaccat tatgtatcta aatctacttc tgtaatatgc
     4621 taacacttca tcattagcct tgaaaagttc tacagtttta agtattaaat catcaaagtc
     4681 aagtgcacta tttcttttta aacggtcttg atatagcgca taaatatctg caatcttaac
     4741 cattctatta tctgccatat tgatatcttt aaactgcttt ggagtataaa gtttatcttt
     4801 tgctcctgaa attgcactta taactgcttt tggttcaaac actttatcac ttaaatttaa
     4861 ttcttttaga cagtctttaa ccaatgtaac ttggtctgct gaatcataaa ttacaaaact
     4921 tctattgtaa ccaatcttat ttatatcttt tcttagtata cgaacacagc aagaatggaa
     4981 tgtacttatc cacatatcct tagtttcagg acctatattt tgttcaactc tctctctcat
     5041 ttcatttgca gctttgtttg taaatgttat agctaatata tttggtgctt gtacaccttt
     5101 atcttctata agatatgcta ttcttgttgt taatacctta gttttacctg aaccagcacc
     5161 agccagtata agtactggac cttcagtctt ttctactgct tctctttgag caggatttaa
     5221 tgtatctaaa ttcatatttc tcctcctgtt aacagccata aatatatttt aatctaaaat
     5281 atgaattact tcaaattata attatgtatt atattaatag attatttcca atatattaca
     5341 attaaataac atttttccat ataatttatt tcattgttta ataaaaaaaa taaccttatg
     5401 atttggggaa tcataaggca cctttaaggg gtctaggtta agggttatta tatataaatc
     5461 tattttttta aaataggttt agggcttaat tatttatttg tattaagttc tctttccttc
     5521 caatacttat acaaatcttc ttctttctta aataagggta aattcatatt tctatgtact
     5581 tttactaacc atggggattc taggccagct tgtataatat tattttcatt tataaacaca
     5641 ttttctgctt ttccttcatc aatcaaaatc cccttatcca atacatatat gtaatcacat
     5701 atttcataca ttaaattcat atcatgactg gatattacta tttttatatt atttttattt
     5761 aatcccttta ttatatctac aatagaccta gtacttactg ggtctaaacc agctgtaggt
     5821 tcatcaagta atacaatttc attttccatt gctataactg atgctattgc cactcttttt
     5881 ttctgtccat agctaagaaa atgtactggc ctgtctataa aatctattcc attaacagct
     5941 actagagctt tgtttattct ctctttaatt gttttttcat ccataccaat gtttctcatt
     6001 gcaaaggcta tatcatcata tacttttgaa taaaaaattt gtttttctgg gtcttggaac
     6061 acaataccta cattttttct aaggttgtat aaacttctct tatcatattt taacttttct
     6121 tctttaaaaa gaatttctcc agatgtaggc tttaatatac ccattaaatt cataaataat
     6181 gttgattttc ctgaaccatt tgaacctatt atacctataa catttccctt tgaaaaatcc
     6241 atattaatgt ttaatagtgc attggtattt ttttcatatt gatacgttaa attatttatt
     6301 ttaaacatca ttatccccta ctatgtgaaa tttaccatca tacaatttta tgtctaatga
     6361 tatacacata tcatcatacc ttgtcataag tcttttgtac agcatacttc caagaatacc
     6421 taaggattta tatgaagttt ttaaatttat atatccaaat cttagctctt gtgacttgta
     6481 tatttctgaa aattcttcta aaaatatgaa tataaaccta tatattaaca ttgataactc
     6541 gacaaaagtg tcaggtaaat gaagtttttt aaaaaagaat attaattgat taaaaggtgt
     6601 tgtaagtata caaaaataca cacaagtcaa acaagacatc gctctaaata acacatgaat
     6661 agacatgtca atagacgcct ttgatattcc tacattaaaa ctaaaaaatt caaaactata
     6721 taataaatct gctttattaa aagatatatt tacaagagtt attcctatac taagaaataa
     6781 aaagtacatt ggtattctaa gtaattttaa ataatttttt aaatctattc cagccacaca
     6841 aacaaccaaa atactcatta aaatcataat tccacttaaa ataaatatat tttgtattac
     6901 catagaagca atcagaaaaa ttactcctat ggctactttt ttgtttggat ttactttact
     6961 caatctattt gtatatgcat atttatcaat tataagcatg attaacttct ctttcctttt
     7021 tgaagaccta atatgtatcc tattacacca gcccctaaag ctgcttgtgc agagaataat
     7081 aaactttcta tttctccact tggtggctca tatggactgc taaaccatgg tttgtaattt
     7141 ggatttatct tagtaatttc actttcagct tgaccatctg ctcctccata ttcagcacct
     7201 gagttgacaa gtaatgggaa tataataaga gctactacta ataaaagtaa taatatgtta
     7261 gtttttgttt tagcactcat tagctttaac tcctttatca ttattttcta gtatgctttt
     7321 ttctccgttt tctgtaatta aattatatac aattacagta agtatacctt ctgctattgc
     7381 tattggtatt tgagtcataa agaaaattcc taaaaattta attgctgaag ccattacccc
     7441 acctgatggg tctgggaaag ccagtgctaa ttgtattgaa gttattgtat atgtggctaa
     7501 gtctcctatt gctgctgcaa gaaatactgg aatagcatta tttccatctt ttttctttaa
     7561 agccttaaat attaggaatg atacgattgg ccctattatc gccattgaaa aagcattagc
     7621 tcctaaagta gttattcctc catgtgctaa taacaatgct tggaatatta aaactatagt
     7681 tccaagaaca aacattacgc ttggtccaaa cattattgct ccaagaccta caccagtagg
     7741 atgtgaacaa cttcctgtta ctgaaggtat ttttagtgct gataatacaa acacaaatgc
     7801 tccacataaa gctaataata ctttcttttt agggtcttgc tttactattt tacctattga
     7861 ttttaatccg aataagaaaa atggtataaa tataactccc catgctatag accattttac
     7921 tggtaagtaa ccttccatta tatgcattgc atttgatgta actggagtta aaatgattaa
     7981 cataagtgca gctataaccc ctaatttaat attctgtttc atcttagtat ccctctctcc
     8041 ctttctaata attaaaacat aataatacat gtctgattta tttaataaaa taaaaaagcc
     8101 taactcgatg gttaggcttc taattttcca caatctaaat acaaacacat aaaacaaaca
     8161 tatctgtata attattataa gaaactttct ccatccccga agaaattact ttaatatttt
     8221 aggcaggtct cctgacttag cttcatccta ctcctcttcc ttcccatgag atataaaaaa
     8281 tatcatcata gtggctaaca aaagtttcgt ctgcatcaca gtagcggggg ctgtaaagga
     8341 atttaacctt tttccctatt aatcttgctt aaagaaccta taatattata tatttaattt
     8401 ttaaattctt cttaaattat tttctatata aatcattgta taccttttct gtgaaaattt
     8461 ctacattttt tataaatttt attcattttt atatttttat gtatataaat tcttttttgt
     8521 aagtttatta atttctaatt atcttcttta aatacattga aactacatca gaaaatctag
     8581 ttatatctct tgaatatata aaatcatttc catatattaa tctttcagct tcaagttctg
     8641 attcttttcc tgtaaatact cctaatacta atatattttg ctttctagct tttctcactt
     8701 cattggctgt atctctcaaa ccaactatac ccctataagc cttttctcct cttaatgttc
     8761 tctcactact ttttcctatc tttacatcat ttggtcttcc atcactgaga actatcagga
     8821 ttttattttc ttcatctcta tttattagac cactacatat agattttatt gccaaaccat
     8881 ctctattatt accagcacaa aaatattcaa atatattttc actatttttt atactatcat
     8941 cataatcttt aaatcttttt aatattgtat agtctaaaaa actagaaaat cccatcactc
     9001 tatttggtat gccaacttct gttagagcat ttgcaattat gtaagcttga attgccacat
     9061 ttccctgatt tctactttga gagccacttg catccaataa tatatcaata acgtatttcc
     9121 ctttttcatt ttctatatct ttataaaata ttttattatt attacttcta ccaactctcc
     9181 aagctctatt ggctaatata gtaccacaat cagaatatac tctactttta gcattttctt
     9241 ctattaaaat tcttgataga ctttctttta atctctttat ttgctgtcta tacattctag
     9301 catgttctct aaattttcct aaattatttt ctttttgtct tgttacatat tttagttgaa
     9361 atacattatt acactcactt ctaagaactc catctgtaaa gtgtattcta caaccttcat
     9421 gaacatttct acaattttgt gtctcaattt ttttaatttc acctttactc aaataagttt
     9481 ttccataata atgttctatt tttgaatata tcttatttgc agtgtcttca tcaacatata
     9541 taactcggtc tgctgaattg ctacatttag aatcatcaaa atcccctaca ccttctacaa
     9601 gcatggatga agatattgaa tctatatcac tttcaataat attttcttct tcatcactat
     9661 aaagttcttc atacatgaaa tcagcaaatg tatcaaaatt tacttctaca ctttctacat
     9721 tattttcatc aataagacta ttttcttctc catttaatat gtattcaaaa taagttaaat
     9781 atataacatc taatgctttt aaaatctcca taatatcttg attggtatct atatttctta
     9841 tatcattaac tattcttcta gtcatactat ctaatgctgg gtgcttgtcc atactataaa
     9901 gtacaaatgt gtatttaatt ttatccataa tattatcatt gtgtattttg gtaaaacgtg
     9961 aaacaatatc tttataagca tttcttctta tgtcttctat ccctggccta tctttaatga
    10021 ccttttcttc aactattgta tttaatataa cttgaattag acccagtata atatctttgt
    10081 tgtatccttt tcttattcta tttgtaagat attttttaac tatatcccaa tctacatact
    10141 ttcttctagc acctgcaatt attgcaaaat atagacctat ttcttttgaa gagtaatctt
    10201 tttctgatat atctatactt tcattgtaat caccagctat tgtccatgat aagttattag
    10261 ctctattttc aaattcataa tcatcatata tccatttcat aatctcacct atattaaata
    10321 tgacttattg gaaacacatc tatactctcc catttattag gtattctagt tttaattaca
    10381 tctccaacca tttctttctc atatacatca aaagtctttc ctgtaagtcc catattaatt
    10441 gcaagagttg gttttaatcc tcttcttata gtcttaagag atgccataag accacgtaaa
    10501 tcaatagctt tactagatat ctctccattt tgagacttca attgtaaatc taaaaatatt
    10561 cctgcaaagt gtattagttt ttcttcatca gcatctgaaa attcattttt taaaatcatc
    10621 attaacttat cttcttctat aggaggaata tctattacca taaatctaga taccagtgct
    10681 tcattaagtt cttttgttcc tgcatattca taattcatag tcccaataaa tcttgttgct
    10741 ggatgtagat tgactctctc atacccaggt acatcaatta ttcttctata gtctaaagct
    10801 gaatgtacca caactatagc atcatttttt gccatattaa tttcatcaaa caccccaaaa
    10861 cctccattaa tagcacactc ataaactgag cctcttctaa gtttaacttc attgtctata
    10921 aatgtatctg tacctataag tgttgaactg tctgtattta tatgaaatga tgtattccac
    10981 tgaggtctat taaatatttc ccctagatta tcagcaagta agttttttcc cgttgccttt
    11041 ggtcccgata ataatatatt ttcttcttct aaaatagctg ctatacacat agacaagata
    11101 tctttaccat aaaaataagt tttagacttt gttactctgt attctaaatc tttttctaat
    11161 ccataataat ttctaaaata cagtacatct tcaataagtt tttcatctac cccttgcacc
    11221 cttagattat ctattatatc catattttta ccccttttat ataaatagta tttgtttcat
    11281 ttcttatcac cattttagca taaaaggata taagctaaaa ttgatttttt aatctccccc
    11341 attttaagta aaaccatgca ccattttatc actacaaaat taagagtact acttactgat
    11401 atttcttgat tttattcgtc tatacgtata taatatatac tgataaatta tgaggaaagg
    11461 atgattgaaa tatggaatat atgtcttgcc cagaagtagc aaaaaaatgg ggtatttctg
    11521 aaagaagagt acaaaaacta tgtgaagaaa gtcgcatacc aggagtttca aaactcgggt
    11581 atatgtggct gataccaaag aacgcagaaa agcctgttga tggtagaaca aaaagaagaa
    11641 aggaagtaca atatgaatag cgttttgatt atagatgatg acaaagaact ttgtgctctt
    11701 atgaaaaaat gtgttgagca agaaaattta tctgctatta ctgcacatgg tggtgcagaa
    11761 ggtctacatt tagtcgataa aaatagacac acactctctc ttgtaatttt ggacataatg
    11821 atgccagata tagacggttt tcaagttctg aaaaaaatta gagaaacaag taatattcca
    11881 gtaattatgc ttactgctaa aaatgacgag gatgataaag tttccggact acgtttaggt
    11941 gctgacgatt acctaacaaa accgttcagc ataaacgaac ttatggctcg tgtcaattct
    12001 cttattcgtc ggtatactat gctaaaccct gcttttataa cagacacaga tttcataata
    12061 ataaatggca tgattattga caagacaaac cgaattgttt ctgtcaataa tattcctgtt
    12121 caacttacaa gcaaagaatt tgatttactt gcttttctgg cttctaataa gggacgggtg
    12181 tttaccaaaa aacagattta tacacaagta tgggaagaag aatatgcttt tgacgatagt
    12241 aacatcatgt ctttcatcag taaattgaga aaaaagattg aaccagaccc caaccatcca
    12301 ttttatattt tgacagttcg tggtgtcggt tatcgtttta ataaggaggc ttaacatgaa
    12361 tacaagtgtc tatctgcttt tgatattggc tcttagcctg attattatag cgtaccttgt
    12421 ttctgtcctg ctacgtgtcc gaatacaact tacacttata aaagatgctt tagaggatat
    12481 aaaaaatgga aaccttaatc gacgtatttt aacagatgaa aacgacatta caaaacaaat
    12541 ttgctatgat attaacgaaa tagcaatcaa tagccaattt caacttattc agcaaaaaca
    12601 atcagaacaa gcctacaaac agctaatgac aagtctctcc catgatgtaa aaactccact
    12661 tgcttctttg gtcggttatt tggaagcagt agaaagtaaa attgttgtag gagaggaaaa
    12721 agacgagtat atccatgttg cgtctaataa ggctcattac ttaaagaatt ttgtagaaaa
    12781 tctatttgaa tgggtaaaat tggattctaa ggaacaagtt tttcattttg atatttttga
    12841 cttgaatgaa ttatcccgta atattatatc tgattggata cctgttttag aaagcagtca
    12901 ttttgaatat gaatttgata taccagaaat agagtacttc ttacgtatag acgcaaatgc
    12961 atatacccgt atcattaata acctattaca aaatattatt acccatagta gtggaaacaa
    13021 aatgacattg agaatttttg aaaacaaaga gcaagctcaa attataataa cagataatgg
    13081 gaaaggtata tcttcagaca atctcccaca tatttttgaa aggttgtacc aatgcgacca
    13141 ttcacgagct tcaaagggaa atggtttagg cttggcgatt gcaaaggaac ttattaacgc
    13201 tcataaggga aatattaccg ccaacagcac tcccggtatg ggaacagaat ttacaatatt
    13261 gcttcctaaa gccctgtaaa agtacgaggc tttcttactc taaaacaaga taaaagcaag
    13321 attatggcaa ggttttggca aggttcatgt tttataattc taattgtagt tgattttgac
    13381 gttgtatcaa ctatcttttg tagaaacaaa tttattaaaa gtgaggaaat tatatatgaa
    13441 tgattttgtg attgaaacaa agcaacttac aaaaatttat gggaatcaaa cagtagttga
    13501 caaagtcaat ctccatgtaa aaaaaggccg aatatatggt ctgttaggac gcaacggagc
    13561 tgggaaaacc acaattatga aaatgatttt aggattgact tcaatcacgt atggcgaggt
    13621 agatgtattt ggagagaata ttaagggacg tgaaaaacaa gtatatcccc gtatcggtgc
    13681 aattatcgaa acgccaggat tttatcctaa tctgacagga acggagaact tggagatttt
    13741 tgcaaaactg cgtggtacag ccgcccctaa ttctgtaaaa agagcattgg aaattgtagg
    13801 cttaccttat aaaaataaaa agctgtttag taaatattca cttggcatga aacagcgact
    13861 tggtattgcc aacgctatcc tgcatgaccc agaactgtta gttttagatg agccaaccaa
    13921 tggtcttgac cctattggta ttgcggagat gagaaagttt attagggact tgagcgttga
    13981 gcatggaaag acaattctta tttccagtca tattctttca gaaatctcat tgttggctga
    14041 tgatattgga attatcgacc acggtgttct tttggaagaa agtagtatga gtgaactaga
    14101 aaagaaaaac agtaaatata tcctgctaca agtttcagat attccgaaga catcacttat
    14161 tttagagcgt caatttaaca cgaaggatta ttccgttcaa gatgactaca cgttacacct
    14221 ttataacact aatctggata tgggtgaaat caataaagca cttgtaatgc agaatgtaac
    14281 tgttattagt tctcaacttt gtaatgatac tttagaggac tactttaaaa aaatcacagg
    14341 aggagagggc attgcttaaa cttattcaaa cagaatttct taaactgcgt cgtaggaaac
    14401 ttatttggct gatgttgttg tctgctctca ttatgccgtt tttcgcacta ctatacttca
    14461 attattttgg taaaacaggt gttgagccaa taaagtttta taaatggtct gcatttggtt
    14521 atacactttg gattattttg cctgttgttt tgggtatatt ttgcaccatg cttatacacg
    14581 aagaaaacca gtatgatatg ctcaaacagc tttggattgt acctattagt aaaatgggat
    14641 acttttttag taaatttttc gttgttttga tttactctat ttgttttatg ctgattacag
    14701 ccgttgcttc tgtgttattt agtgtacttt ctggatatgt cgtatttagc tgggcaagca
    14761 ttttatattt gttgaaaaaa tgtttagaaa ttggtgtaat cactgctttt gtaatgttgc
    14821 cgattttggc aatcgctaca tctcaaaaag gatacattct cccaatttgt atcacattgg
    14881 tatatgcctt ttccggtttt gttataatga caataaatat gtatttacac ccattatcca
    14941 gtatgggagt tattatcatg agaaataaag acattccagg tcttatattt tcacaggaaa
    15001 ttaatgtccc attagcattt ctttgtattt gtatttggag tattgtatca gtattgtttg
    15061 caaatattgc actaggaaga agaaagtgag gtgtagataa tgtggaaatt actttgggct
    15121 gaatgtcaaa aaattcgtcg ttcaaaaatt gtttggatta cagtttttgc atccattatg
    15181 atagcagtca tagtttttgc atgggggcag gatgtatata atgacccaga tttacattat
    15241 ggcttaaaga gcgtttatga cggttcacgg tatatagaca atgcaggttg gtatatggac
    15301 gcagtacaac cttggtcaac tttttttgtt cttcctgctg tcattgccct attaggaagc
    15361 tatattattt gtcgtgaaga agaggaggac actattaaat ccctgcgtct tattcccata
    15421 aatgaaacaa atttaaccat agcaaaaatg attattactt ttatattcag tatacttctt
    15481 tatttgttac tctttactat aacatttttg actgaatccg ttttacattt ttccgattta
    15541 tcgacaaaat tggttttaag ttgcatgaaa gagtattttc ttgatggcat aggtgtattc
    15601 tttgcaattt ctcccattat tgccttggta tcgcgcatga aaaaaggata ttggcttgca
    15661 ttagtattca cagaagttta ttctattgct ggactatttg cgggtatgtc aaatgtctta
    15721 caaacctttt ttcctattgc ggcaatattt aatatttctg gatactatat aactactgga
    15781 gaaaaaatta tggggagtat catgagttta ttactttgtg cctgcctttc tgcatttata
    15841 cttaaagggc taaaacacaa tgaaaaaaat tgaaataggt atatcaaaat gaaaaagata
    15901 aaaaaactga ttattctaat tgtattcttg tttctttttg gcatgttaca ctgttatact
    15961 gaatggaaat tcaacaataa cccacctaaa ggggtaatag aaagaataaa tgagtggact
    16021 gataagattg gactggatta gtaacttatg ttaattatag tgaatgtaat attagcaagt
    16081 tattgtttcg tattttaata ctataacttt aaatatttgg ttttaatctt ccaaacgaat
    16141 ataaaaaaac tgtcacagag gcaacatatt tttgaatatt tataaagtgg tagttttgat
    16201 tttcaaagct accgcctttt cttctatata agcaaactta tcctcaacaa agtgtaaaac
    16261 atggtgcatt ttaagaaaaa agtaccacag acacaagcaa taaaagaaac gccgagaacc
    16321 tacaaaaacg tagtgcttat gcagatttac gacgccttct attttctcaa ccgtcttaat
    16381 gaccttgtac aaaagaaatt tttctaatat tttttataat cttatcttgt gatgtttttt
    16441 atgtataaat gacttaataa caaatccaat aaaaattatg gatattggta taattagaac
    16501 gaggaattta taatcattat aatgtgacat tattcttacc caactctcag aaaaaatata
    16561 tcctatactg attaatgaag tattccatat ggttattccc acgatagatg atattacaaa
    16621 agttataata ttttgacctg ctatacctgc tataaaagat atgtatgttc tacataaagg
    16681 gattaatcta catacacaaa cagaaataga agagtattta ttaaaataat cttgagatgc
    16741 aaatattcct ttctttgcct ttggacatat ctctattatt ttatttatga tagatttacc
    16801 tccccaattt cccactgtat aacaaaatat cgaaccaagt atacctgcaa ttatacttaa
    16861 aattaaaatt gtcagaaatc caaaatgatt tcttgcagca attgctccac ataaaggtaa
    16921 aacaacttcg ctaggtaatg gaaaacaagc atactcaatc ataattaata caaatacaga
    16981 gattaatcca tattgtaaaa taaaactatt gattacagac acttacttat ctatctcctt
    17041 tcttatttaa aggttttaag ggtacatgtt ctaaggattc tcaccctata tttaaagaat
    17101 caatctcttt aaactattat atgtatttac acaaagcatt tataacatac tttaaaaata
    17161 gtaccttaaa ataagaaact taataaataa aaaaagatta cgtggctacg tcctactctc
    17221 ccaggcag
//
LOCUS       DAMKMI010000335        16160 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.guided.092, whole genome shotgun sequence.
ACCESSION   DAMKMI010000335 DAMKMI010000000
VERSION     DAMKMI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.guided.092"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            227..844
                     /locus_tag="PC429_003908"
     CDS             227..844
                     /locus_tag="PC429_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF3849301.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            952..3483
                     /locus_tag="PC429_003909"
     CDS             952..3483
                     /locus_tag="PC429_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF3849302.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3917..4618
                     /locus_tag="PC429_003910"
     CDS             3917..4618
                     /locus_tag="PC429_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF3849303.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4668..5750
                     /locus_tag="PC429_003911"
     CDS             4668..5750
                     /locus_tag="PC429_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF3849304.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6011..7111
                     /gene="vanG"
                     /locus_tag="PC429_003912"
     CDS             6011..7111
                     /gene="vanG"
                     /locus_tag="PC429_003912"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF3849305.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7108..7914
                     /locus_tag="PC429_003913"
     CDS             7108..7914
                     /locus_tag="PC429_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF3849306.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7932..10070
                     /gene="vanT"
                     /locus_tag="PC429_003914"
     CDS             7932..10070
                     /gene="vanT"
                     /locus_tag="PC429_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF3849307.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10384..11004
                     /locus_tag="PC429_003915"
     CDS             10384..11004
                     /locus_tag="PC429_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF3849308.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11170..11706)
                     /locus_tag="PC429_003916"
     CDS             complement(11170..11706)
                     /locus_tag="PC429_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF3849309.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11819..12493)
                     /locus_tag="PC429_003917"
     CDS             complement(11819..12493)
                     /locus_tag="PC429_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF3849310.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12696..14570
                     /gene="asnB"
                     /locus_tag="PC429_003918"
     CDS             12696..14570
                     /gene="asnB"
                     /locus_tag="PC429_003918"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF3849311.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14662..16098)
                     /locus_tag="PC429_003919"
     CDS             complement(14662..16098)
                     /locus_tag="PC429_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF3849312.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
BASE COUNT         6163 a         1766 c         2707 g         5524 t
ORIGIN      
        1 ttcatgaggc actctttttg tatgaaatca aatatatttt tattatttca acaataaaaa
       61 agaggtctgt ttctattttg agacaatatt atttttatgt agatttaatc ttaatgagta
      121 taaagtttta aaactactaa aataggagga attttaatat aaaagggtaa ataaagggtt
      181 tagagggcat aatatagaat aaattaatta tttaaggagg gtttttatgt gcaaaaaaat
      241 gtatttatta gtggtaggta tacttatagc aggtttatta actgcatgta atgggagtcc
      301 aactaaggat tcagaacaag cacaagataa tacaaaaaag gaggcaaatg tatcaaataa
      361 cgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa caagatttta aagtgccata
      421 cacagatgca ataaacatat ttaaagacaa atacaaagat gctgatatag tagatttaag
      481 tcttgagaga gatttaaata aatttgttta tactgtggaa ggtgtagatg ataataatga
      541 atataagatg aaaattgatg ctaatacaaa agatgtatta gaagataaaa cagaaaagtt
      601 ggattcagaa gatttaaatg gagtcgctag aaaagaaaaa ttagatttta atgacataat
      661 gacacctcaa caagctatgg aaattgcatt aaaagaacaa aatggaatgg ttaaagagtg
      721 gagtttagat aaagatttag atgttacttt ttataaaata agaatagata aagataaaaa
      781 tgaatatgac ataaaagtag attctaaaaa gggaactgta ttgaaggtag aaaaagaaga
      841 ttagaagatt taaactatct ttaaaataat ttcttttttg aataaaattt gtttaaaata
      901 taacaagtag ggtataaaga gactaaataa ctattttagg aggagtacgg tatgtcattg
      961 aaatcgctaa aaataaaaga aaatttatat tgggtaggtt cgcttgaccc tgatttaaga
     1021 gtatttgaca taattatgta tacaccttat ggaactactt ataattcata tgtattaaaa
     1081 ggaacagaaa aaacagtttt atttgagaca gttaaagata agcattttga caattatatt
     1141 gaaagattaa atgatttaaa tatagatttt gaaaaaatag actatattgt tgtaagtcat
     1201 actgaaccag accatgcagg aagcgttgaa aagcttttgg atttagctaa aaatgcaaaa
     1261 gtagtagctt cagaaacagc aattaaatat cttaaggaaa tcgtaaacaa agactttgaa
     1321 tatgttgcag ttacagatgg agatacatta tcaattgggg acaaaacttt agaatttttc
     1381 tctgtaccta tgcttcattg gccagatacc atatacacat atataaaaga ggataaaact
     1441 cttgtaacat gtgattcatt tggtagtcac tatagtaatg ataagatagt aaatacactt
     1501 gatgaagatg aagaaaagga ttacttggat gctttgagat actattatga ttgtataatg
     1561 ggaccattta aaccatctat ggtaactgcc atagaaaaga taaaagattt agatatagat
     1621 actgtatgtc caggtcatgg tccagtatta actgaaaatc ctagaaaaat tatagacctt
     1681 tattacaact ggagtgtaaa tgaacaaata aagttagaaa aagaagttac aatttgttat
     1741 gtttctgcac atggatatac aaagattatg gcagaagcta taaaagcata tattgaaaaa
     1801 aatagcaact ataaagtaaa tttatttgat gtaatagagc ataagcaaga agatattttg
     1861 gcaaaaatag cagtatcaca aggtgtatta tttggaacac caacaatatt aggagatgcg
     1921 ttaaagccta tatgggatat attaatatcg ttaaacccag ttcttcatgg tggaaaagtt
     1981 gcttcagtat ttggttcata tggatggagt ggagaaggta tagaaaatgc tatggagaga
     2041 ataagtcagc ttagaatgac agcagtaaaa ccttttgctg ttaactttaa accatcaaat
     2101 gaagagattg ataaattaca ttcttataca ggtaaattcc tagataagtt aaattctact
     2161 tttggaagta aaaagaaaac taagaaattt aaatgtgtaa tttgtaatga agtttttgaa
     2221 ggtgatagtg ctcctagtgt atgccctgta tgtggagcta aggaagacca atttatagaa
     2281 gttgaagaag atgaagttac ttttagaaaa gatactgatg aatatttcgt aatagtagga
     2341 aatggagcag ctggttttta tgcagcagat gctataagaa aaagaaataa aacttgtaag
     2401 ataactatga tttctaatga agatgagttg acttactata gacctgcact ttctgatggt
     2461 ataaatgaag aacttggttc agatttctac atggaagata aagattggta tgataaaaat
     2521 aatatagtag taatattagg aacgaatgta gataaattag atgaagtgaa taaaactatt
     2581 atagtgaatg atggagctat taaatttgat aagcttgtaa tagctacagg aagtagaaac
     2641 tttataccac caattaaggg tcatgattta gaaaatgtgt ttactcttag aaatataaaa
     2701 gatttatata gtgtaaaaga agcactagaa aaatctaaga aagttgtagt gattggtggc
     2761 ggtttattgg gacttgaagc tgcatgggaa tttagactaa aaggattaga agtagtagta
     2821 gttgaagcca tggatagcat actatcaaaa caacttgata aagaaggcag taaaatatta
     2881 gaacaatgtg taagagatac aggaatagat gtaagattgg gtgtagcagt tgatggtatt
     2941 gaaggtgatg gaaaagctca aaaagttgta tttaaagatg gagatagtgt tgactgtgat
     3001 atggtagtat tttcaattgg tgtaagagca aacactcaaa tggttcagga tacatctgta
     3061 aaaattgaca gaggtattgt tgtagataag acattacaga caaatgttaa agatatatat
     3121 gcttgtggag atgttgcaca agtgggaaat attagtttag caatatggcc atcttcagta
     3181 gaaatgggaa aaatagcagg ggcaaatgca agtggagata atttgacatt tgaaagtgaa
     3241 gtatatccag tttcattaga tgcaatgaat gtaaaagtat ttagtattgg taatatacaa
     3301 aattttgata aagaaatttc atcaaaagat gaaggtcaaa gaatatataa aaaattattt
     3361 atgaaagatg gttcattagt tggagctata cttataaatg atttaagctg tactgttaaa
     3421 ttgataagat taatatctga aaaaggtgat tttgaggata ttatgaaggc tgatatacta
     3481 taatttaaat acattaaaat aaaaaggagt tgtcttaaaa ttgatttttt tctaatcaaa
     3541 ttttaaattc aactcctttt ttgaattttt atataaaata acatggaaat tttagtaatg
     3601 tctgtggtta catacatagg tttaattgtt ttttatatgg attgtctctg gtataataaa
     3661 ttgaattgtg tttgactgat agatattttt attgaaagtt aatacctgtt atgcgataat
     3721 aaggacgtgt taggggagtt tttataataa caattaatct cagtttttgt gttccaatag
     3781 tagcatcaac acattctgat gtagtagttc ttttcttata aaaattagtt acaaatatgc
     3841 ttgtaattat ttgatatgta aatttaccaa tattataaat gatgacctcg ataataagga
     3901 gagtgaaaaa taatctatga acgaaaatat tttgattgtg gatgatgaaa aagaaatagc
     3961 agatttaatt gaagtatatt taaaaaatga aggttttaca gtatacaagt tttatacagg
     4021 taaggaagct ctagaatgta ttgaatctaa aaatttggat atggctattt tagatattat
     4081 gcttcctgat attgatggat ttcatatttg ccagaaaatt cgagaaaaat actattatcc
     4141 tatcattatg ttaacagcta aaattgaaga tatagacaag ataatgggtt taaccatagg
     4201 tgctgatgat tatataacaa agccatttaa ccctttggaa gttgttgcaa gagtaaagac
     4261 acaattaaga agatatatgc gatataataa ttcttatgaa caacagtcga taattgttaa
     4321 tgagtatgat attaaaggat tgattatcaa taaagaaaca cataaatgta gtttatttgg
     4381 gaaagaagta gcattaacac cgattgaatt ttctattctt tggtatttat gtgaacatca
     4441 aggaaaagtg gttccttctg aagaattgtt tgaagctgta tggggagaaa aatatcttga
     4501 taataataac acagttatgg ctcatatagg gcgattacgt gaaaaaattc atgaaccttc
     4561 taaaaacccg aaatttataa aaactgtatg gggagtaggt tatacaattg aaaagtaaaa
     4621 atattaaagt caatcctgac tttactcaag tgagaaataa aatttttatg agaacagctt
     4681 taatggcatt tatagccatt attttcatat ggattttata ttcttttata ttgtatggta
     4741 agttttctat ctggatagta tcattctttg aaaatgcttt tcgtatggaa tataaagctg
     4801 cattgaattt atatcagcag attttccgta atagtatgga gctcttcatg atactagcta
     4861 tggtgattgt gttttttata atattccgtg tttatttaaa ttggtttaca aaatatttta
     4921 gtgaaataaa tcaaggtatt gactctctta tcaaagaaga tgttggagaa gtggctttat
     4981 ctccagagtt attggcaata gaaaagaaaa taaactcaat taaacatata cttgaacaac
     5041 gtaaatttga gacacaaatg gcagaacaac gtaagaatga acttattgtt tatcttgcac
     5101 atgatttaaa aacaccactt acatcagtta tagggtatct gacactactt cgagatgaaa
     5161 gtcagatttc tgaagaactg cgaaaaaaat atttatctat ttcgttggat aaggcagagc
     5221 gtcttgaaga tttaattaat gaattctttg aaattacaag gtttaattta tcaaatatat
     5281 cacttgaata cagtacagta agcttaactc gtatgcttga acaactaaca tatgagttta
     5341 aacctacttt gatgagtaag aatttaaaat atacactgaa tgttacacca aatatgatga
     5401 taaggtgtga tgttaataaa atgcaacgtg tatttgataa cttattacga aatgctgttt
     5461 attatagttt tgaagatact actattgaaa taacagctgt acaggaggat gattatataa
     5521 agcttaaatt tataaatcat agcaatacga ttccagagga aaaacttgag cgtatttttg
     5581 aacaatttta caggttggat acatctcgta attcaaacag tggtagagct gggttgggac
     5641 ttgctatagc taaagaaata gtggagcttc ataacgggac aataacagct catagcgaga
     5701 acaatataat tacctttaag gttattattc cgattgtagg aaatttgtaa gaatttaagt
     5761 ataaaaaaat aggaaattct ttaggataaa taaataaaga gcactcttgt tattgatata
     5821 attactgtat caaaacaaga gtgcttttat tttgatacag taattatatc aatagaaaaa
     5881 tgtgaaaata tattatgtgg aacagatgca attgtaagtg aaggttgatt ggatttgaaa
     5941 tagaaaaagt attttaacat gtttctgtag attgtataaa aataaataaa tcaacaaagg
     6001 agaatcaaag atgaataata aaaaaattgc agttatattt ggtggaaact caacagaata
     6061 tgatgtatca ttacaatcag cttttgcagt acttgagaat ttgaatacgg aaaaatatga
     6121 tattatacca attggaatta caagggaggg taattggtat cattatattg gagattataa
     6181 taatatccag aataacactt ggtttgaaag ggatactgat ataattccag ttattgtttc
     6241 gcagaaccgt gtcaaaaatg gtattattga atttttaatt cctgatgctg tttctcagaa
     6301 tcatattcca aatagtgcta ttgaatcatg tgaaaatgat attaaataca ctaaacttga
     6361 ttttgtgttt cctgtactgc atggaaaaaa tggagaagat ggaacagtgc aggggttatt
     6421 tgaattggca ggtatacagg ttattggatg tggtactctt tcttcagcat tatgtatgga
     6481 caaagacaaa tcacataaac ttgttcattc agaaggtatt aaagtaccaa aatcaatatt
     6541 actaaaatac tcagataaat ttaatgtggc tgaactgtca aaagacttaa cttatccatt
     6601 atttataaaa cctgtgcgtg caggttcatc atttggtatt agtaaaattt ataaaaaaga
     6661 agagttacaa gtagcagtag acttggcttt tgaacatgat aatgaagtaa taattgagga
     6721 aaatattgat ggttttgagg tagggtgtgc agttttagga aacaatgact tacttgttgg
     6781 aagagtagat gaaatagaat tgtcaaaagg attttttgat tatacagaaa aatatacatt
     6841 gaaaagttca aaaattcata tgccagctag aattgatttg gattgtgaga aaagaataca
     6901 agagacagca aaaattattt atagaactct tggatgttct ggatttgcta gagtagatat
     6961 gtttttaact ccatctggtg aaattatttt taatgaagtc aatacaattc ctggctttac
     7021 ttctcatagt cgttatccaa atatgatgaa aggtattgga ctgtcttttg aagaaatatt
     7081 ggacaagata attgaggtgt acataaaatg atgaataata ttttattaga taaaaataaa
     7141 gaagataaaa tgacaaatac tatcttattg actaaagata aagtttatga tgggaatcta
     7201 attctagtaa atgctttttt accagttaaa acatctgaag atatagattt aattcctgta
     7261 gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta caaacatttt acaaaatatt
     7321 ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa gtggatatcg tactgcagag
     7381 gaacaacaag atatatattc aagttctctg cgtgacaatg gaaaagactt tactaaaaag
     7441 tttgtagcac ttccaaatca cagtgaacat cagacagggc ttgctattga tttagggtta
     7501 aagaaagaag ttatagattt tatctgccct gaatttcctt ataatggtat ttgtaatgag
     7561 tttcgtaagg cagcactaca ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa
     7621 ataacaggaa tatctcaaga accatggcat tttcgttatg tgggatatcc gcattctgag
     7681 attatcaata aaatgaatct cgctcttgaa gaatatatag aatatattaa gagattttcg
     7741 tatacaaatt gtttaaaaat agaagataaa gaaaaaatta tagaaatctt ttatgtgcct
     7801 attcatacta aagaagaagt gagaatctct atacctgaac aatgtgtata tcaaatatcg
     7861 ggtaacaata aggatggttt tattgtgaca ttttggagaa atagaaagga ctaagaaatg
     7921 gaggaacaag aatgattagg aataaatctt atgcagggct tgattatttt aggattatag
     7981 ctgcattact tataattgca attcatacat ctccattatt atcatatagt aaaatggctg
     8041 actttgtgtt aactagtata attgctcgta ttgctgtgcc attctttttt atgctatcag
     8101 gcttttttct aatttcaaca tataattata attgtaataa actgaaatct tttctaaaaa
     8161 atgtattatt tatttacggg atagctattg taatttatat tcccttaaat atttacactg
     8221 gatatttttc catgaataat ttagtgccca atattattaa ggacattgta tttgatggga
     8281 ctttatatca tttatggtat cttcctgcat caattattgg tgcaagtatt gcatactttc
     8341 ttgtaaaaaa actaggttta aagtactcac tagtaatcac tattgttatt tatgtaattg
     8401 gtatgtttgg tgatagttat tatggttttt tagaaaaaat accactatta aagaatttat
     8461 atgggtatat ttttgaagta tcagattata cacgaaatgg tatatttttt gcacctgtat
     8521 ttttcatact tggtggaata atatctaata aatctaatta tatgtcaact aaaaatagct
     8581 tgattggatt tcttatatca ttcttactta tgttatgtga gggaatgttt ttacataaac
     8641 taggggtaca gcgtcatgat agcatgtata ttatgttatt gccatgtatg tatttcttat
     8701 ttacagcctt aacatttttg aaaggttctc gagctaggtt attgcgaaca tcatcattaa
     8761 ttatttatat tatacatcct atgatgattg ttgttattcg tatgttttct aaaatattcg
     8821 gaatacaaac tgtcttggtt gacaatagcc ttattcatta tatagtggta agtatagcat
     8881 cttttatagc ttctataatt attatattga ttcttaaacc tctaaaaaaa gacttgaaag
     8941 gagaacataa ggtaaatacc aatagagctt ggattgaaat tgatttaaat aatttggaac
     9001 ataatgtaaa gatgatacaa aaagctatgc cagaagattg tgaattgatg gcagttgtaa
     9061 aagctaatgc ctatggtcat agtgcttttg aagtagcgac ttgtgcaaac caaataggtg
     9121 taaaagcatt tgcagtagca acaatagatg aggggattga tttacgtcga tatggaatac
     9181 taggagaaat attgattctt ggatatacta acccaataag ggcaaaggaa cttcataaat
     9241 acaatcttac acagagtgtt attgacctta atcatgctat ctctttaaac aaacagaaat
     9301 acaacataaa atcacacatt aaaattgata caggtatgca cagactgggt tttgatgtta
     9361 atgatgtgag gtcaatttta aaggcattta aattaaaata cttagatata tgtggcatat
     9421 ttacacactt gtgtgtttca gatagtctga aagatgaaga tgtagacttt acaaataaac
     9481 aaattgaatg tttttataag ttaattgatt ttctgttaaa aaaaggtata aaaattccta
     9541 aagtacatat tcaaagtagt tatggttttt ttaattatcc caatcttaag tgtaattatg
     9601 taagaattgg aattgcatta tacgggatat taagttttcc aaatgatacg actaaattgc
     9661 aacttgattt acgacctgtt ttatccttaa aatcacaagt aattcttatt agaaaaatac
     9721 aaaaagatga tagttttgga tatggaagag tctttattgc aagcagagat agcgtaattg
     9781 ctatattatc tattggctat gccgatggta tacctagaaa tttatcctgt ggaaaaagtt
     9841 acgtaataat aaatggttgt agagcagcaa tagtgggtcg aatttgcatg gaccaacttg
     9901 ctgttgatat tacagatata cctaatgtag aagttggtaa tacggcaata ataataggaa
     9961 gagataattt atctgaatta tctgtttctg aagttgcaaa taactcatgt agtatatcga
    10021 atgaattatt gagcagagta ggaagaaggt taaatgttat aaagaagtaa ctcaattaaa
    10081 tagtaatatt tgttgtactg tgtaatttat tttactctga ctagacattt ttagtaatat
    10141 tatgtctaat atatattttt tataacttac atgacaattt aagttataaa aaggtgatgt
    10201 ttcagatttt tattttgaaa catcaccttt tttgttataa tatataataa aataattata
    10261 ccttaactgt aaacttatat agattattta ctttttaata ttagattcat tttttgagat
    10321 tggtatataa taaacatata gagtatataa taatagttaa agttaaaaaa ggggaaaagc
    10381 aatatgcagt atatacaaga actacttgat ttgactcaaa attattgggt gttagccaca
    10441 ataatcggct tattaagtgc atttatagaa agctttatac cagccttacc acttgttgct
    10501 atagttacag caaatgctgc aatacaagga ttatttattg gatgtttgtt atcatggatt
    10561 ggctcaggac ttgggacaac ttcattattc ttattaataa gcagatttaa tgatagtaag
    10621 ctttttaata agttgagaaa tagtaaaact gaaaaagcaa taagttggat ggataaacag
    10681 ggatttaagt tattatttat agcgtatgcc tgtccattta tgcccggttg tttagttact
    10741 atcgcatcag cttttggtaa aaaagattta aaagattttg tgccagcaat gttggcagga
    10801 aaatttgtaa tgtttatagt tataagttac gtggcaagtg atatagaagg ttttataaca
    10861 agtcctttaa agatagcatc atttatatta ttggtgtttt tgtcatggaa gattggaaat
    10921 agagtaaata aaaatcttga aaatcataat tatgatttcc atcataaaaa acatcacaat
    10981 gataaagatg ataaaatgat ttagcttgta cgaaataaaa cgtatcttaa aatgatttag
    11041 tactaaatta ttttaagata cgttttattt tttgtattaa ggtacaaaat ttttagttct
    11101 tttgtaaact aaaatatata aaacttattt atacttagtt caaaatattg ttatttattt
    11161 tttctacaac tataataaaa tataaaattg atatccaatc aaaaaaataa ctatgaaatt
    11221 taatagctta gatatgaatt tttctgtttt tgagttagtt tttataggta gtaaagaaat
    11281 gtttatagta atgggataaa acaattcaat accttcttta gtaataaggt caagacacaa
    11341 gtgagataga tatcctatta taaacccact ggacaaacat gagaaaacta tattattgtt
    11401 agtgaagatt gtcagagatt taaaaaaact ataaataagt aataaggcaa ttagactgtg
    11461 agtaaaccct ctatgtctaa acctacttcc aaataatttg tatattaaca taaatttttt
    11521 acttatgtaa gaacctctca tatctatatc tggaaataaa gaacctaaat atgcaaaata
    11581 aatataaatt ataattaata ctattttata tgcaatatcg tatttaacta ggtacagatt
    11641 gtttataaag gggagtatca aaagtgcgaa tatataacct cccctttcat gtgtttcttt
    11701 tatcatataa aaatcctcct gaatcgtcac tataaacatt atattggtaa atttagacta
    11761 tcttttatta tattatatag caaacataag tttttttcaa aaggatttta atatttaatt
    11821 aatcttgaga ttttaaattt ttgtaatttt ctaaagcacc attccaattt actacattaa
    11881 accagttgtc aatgtattca tttcttctat tttgatattt taaatagtaa gcatgctccc
    11941 aaacatcaag tcctattata ggagttaggt ttttacttac agggctatcc tgatttggag
    12001 tagtcataat agataatttc ccatctttag tagctacaag ccaagcccaa ccagaaccaa
    12061 agacatctaa agcagatttt tggaactctt gcttaaattt ttcaaaagaa ccaaagtctc
    12121 tatcaatagc ttcttttaaa gattcagaag gtatggtttt ttctggcgtc attatatcaa
    12181 aaaagaattt atgattataa gctccacctg cattatttct tacagttgta gcaatatctt
    12241 taggtaaaga atctaaattt tgcaataatt cacataaaga ataattataa agctcaggat
    12301 atttttcaag agcagcattt aatttatcaa cataagcttg ataatgctta tcatgatgca
    12361 gtttcattgt ttctttatct atataaggtt caagtgcatc atatgcataa ggtaatggtt
    12421 taaccttaaa tttgttattt tcaggtgtaa aagcaaatga agttatgcac tgtgagatta
    12481 taaataaaga cataataact ggtattaata tttttttctt cataaaaatc acctcctaat
    12541 atagtatttc tagaaataaa aaaaatatga aatattaaca gataatttat aagaatttaa
    12601 tagcaatttc attttaaatt gaataagttt taaagccaaa gacaaaacta tgaaatatag
    12661 aataagacta aaggacagaa aggaattttg attatatgga cggcaatatt tcaatttatt
    12721 ataaaggtga tatatctaaa aaagaagata ttaaatttaa aataaaagaa tctgtaaata
    12781 atacaagact ttctagtaga atagagtata aggatgagga aaaaactttt gattttgaaa
    12841 gatttaatgt aattgatgct atttatggaa ataaaccatt tgaaaaagat ggaaaagtta
    12901 ttgtttttaa tggaaatata tataactatc atgaaataaa agaagaatta attaaaaaag
    12961 gacacaattt tactacaaat gatgatattg aagtcttatt ggcatcttat atagaatttg
    13021 gtaagaattg tgtacataag attaaaggaa tgttcaactt tataatctat gatagagaaa
    13081 atgaaagtat atttggagca agagatttat ttgggataaa accactctat tatataaata
    13141 aggaaaatgc aataattttt tcctcagagt ataagttttt acttgaatat atgaaaaatt
    13201 taaatataaa tgaaagaagc ttacaaagtt atttttcatt tcaatatgta cttcctgaag
    13261 atactatgat acaaggaata aggctgatac cagcaggtca ctattttagg gtagaaaatg
    13321 gtatactatc tttaaagaga tataataaac ttgagtttag aagtagtaca aaattttttt
    13381 atactaaaaa tcacttaggc aatagagatg tgaacgagga tgatataaga aatatagtag
    13441 tagattcaat agtaactcat atggaagagg agaaagaaat tggaacattt ctgtctggtg
    13501 gaatagattc atctattata actactgtgg cttcacaaat taatccaaat ataaaatctt
    13561 tttctgcagg ctttagtgtc aaaggatata gtgaattaga agtagcaaag aaaacagctg
    13621 ataagttagg tatagaaaat atccagatta atataacaca agatgaatat ataaaatcat
    13681 taccgaatgt aatttattct ttggatgacc ctatagcaga cccttcagaa gtaggtcttt
    13741 atttcttaat taaagaggca ggtaaacatg ttaaagtagc attgtctgga gaaggagcag
    13801 atgaattatt tggaggatat aatatatata aagagtatag tactatgaaa tcagttgtca
    13861 attcacctac ttatattaaa agtatattag gaagagtttc agaacttatg ccaaacataa
    13921 aaggaagaaa ttatctttat agagctacaa caccattaga aaaaagatat ataggaaatg
    13981 caaaagtttt tgaaaacagc gaagtaaaaa gattcttttt taagtataag gaaaagaata
    14041 tatatgaata cctgttgtct aatttgtata gagatgcaca aaaaaataat tacgattata
    14101 taagtaaaat gcaacatata gatgtgaata catggcttca aggcgatata cttcaaaaaa
    14161 ttagcaaact ttctacagct gagcaggttg aacttagagt accattttta tataaagatg
    14221 tatttgatgt agcaaaaaat ttaagaatgg agcaaaaaat aaataagaat aatacaaaag
    14281 ttttacttag agaggcgttt agagaaatag tcccagaaca tgttgttcaa aggaaaaaat
    14341 taggatttcc aactccaata agagtttggt taaaagattc tctaggggat attgtaaagg
    14401 aaactattta taattcaaat gtagatgaat ttatagataa aaaatatgca ataaaacttt
    14461 tagatacgca tcttaaaggt catagagata attcgagaaa aatatggact atatttacat
    14521 tttgtctatg gcatcaatta tttattgagc ataaaaatgt cgagtattaa tacttttttc
    14581 aatattaatc agagggcaaa agcatgcaat gttttcaccc tcctgattat tatggaatag
    14641 gataagtaga attttgattg attagtttac tatctcaata aaattattgg caaatatttt
    14701 gacaagatta ctttcaacag ctaaatttaa gttttgtcca tcatcatatc cagcggaaat
    14761 caatcctagt aagtttccaa acatatctaa aagagctcct cctgaactac cactagagat
    14821 tggagctgta aattgtatca tttgaaccgt ttcaaaatca cgaaaagctg aaataattcc
    14881 atctgaaact gtattaaaaa gacctagagg gcttccaatt gcaactattt tttgacctct
    14941 cacaggtttt ttactgactt taacaggtat tggcttgcat tttctatcaa cctttatcac
    15001 agctaaatca taatctgaat ggtattttat aatttggtaa gatgtataaa ctttatcatc
    15061 attttcaaat ctaactaaaa atgaataccc atcattgact acatgaagat tcgtaagtat
    15121 ataaccttca ttatttatta ctactccaga accgctctta aacggtttat tttcgttatt
    15181 gcacacaaca atcataacaa cagaagctga taactttgca aggtcttcat aagaaagttc
    15241 tacttcctca tcttgtacaa tttttttaga tgttagattt gtgttattaa gtgtattttt
    15301 agagttaggt ttattcgtag taggagaagt cttattggta ttttcactgt aatttatatt
    15361 tgtaatatgc ctttcaaaag gtatttctaa tatatttgga gtcttcctag aaagttcaaa
    15421 tagagtagaa aattttgaac attcaggata taaatcaaga atatctttat ttggatttat
    15481 atataaaata tcacacccaa gttgtgataa aaaatataag aaaaaaagtt cttgcctttt
    15541 aatatttcca aagtatacta tctttggtga aatagtagtc ttcaccttgt tataaaataa
    15601 tttaggtaga aaatagtcta tccaacacaa aagtttaact gcaaagtttt tgatgataga
    15661 ggatgtacaa tttggcatac ttgtggaaaa taaatctaaa gtatttttaa atgcaatctt
    15721 tttagtccaa tttaaggtgt cattttctat tttacatgga aagtcgatat tatataaatc
    15781 ccaactgtta aacttagaac tattataaat attaaaaatt ttaagcatct tatctgtttg
    15841 ttctttgtca aaatttattt ttagattgtt taatctaata tagggaattg taggattatt
    15901 aaaaaaataa gtatctatat ttttaatttt ttctatgtat ttatcaattg aatcatcaac
    15961 acctatatat cttgtaaagt agacttcttt aatatatgaa gaacgcttat ttttataggt
    16021 agtatcagat ataaattcaa ttagaggttc agtagacttt tttattgaaa tctttaatgg
    16081 tttaatagta caaatcatat tttttctcct ataataagat gatataatct attataaaac
    16141 aacttaaaac aatatagtac
//
LOCUS       DAMKMI010000336        15199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.guided.101, whole genome shotgun sequence.
ACCESSION   DAMKMI010000336 DAMKMI010000000
VERSION     DAMKMI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.guided.101"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="PC429_003920"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="PC429_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF3849313.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="PC429_003921"
     CDS             1575..2258
                     /locus_tag="PC429_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3849314.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="PC429_003922"
     CDS             2255..4915
                     /locus_tag="PC429_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3849315.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="PC429_003923"
     CDS             complement(5096..6277)
                     /locus_tag="PC429_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3849316.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="PC429_003924"
     CDS             complement(6277..6939)
                     /locus_tag="PC429_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3849317.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7071..7709)
                     /locus_tag="PC429_003925"
     CDS             complement(7071..7709)
                     /locus_tag="PC429_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF3849318.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="PC429_003926"
     CDS             7888..8493
                     /locus_tag="PC429_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3849319.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="PC429_003927"
     CDS             8997..9746
                     /locus_tag="PC429_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3849320.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..10507
                     /locus_tag="PC429_003928"
     CDS             9743..10507
                     /locus_tag="PC429_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF3849321.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10606..11427
                     /locus_tag="PC429_003929"
     CDS             10606..11427
                     /locus_tag="PC429_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF3849322.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            11441..13105
                     /locus_tag="PC429_003930"
     CDS             11441..13105
                     /locus_tag="PC429_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF3849323.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            13110..14984
                     /locus_tag="PC429_003931"
     CDS             13110..14984
                     /locus_tag="PC429_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF3849324.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
BASE COUNT         5709 a         1748 c         2526 g         5216 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtattacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaaggc gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atataaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataag
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggatatag aatcttaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctatagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggc tgactgaaaa agatgcatca aaagaacttg cgaaaaatgg
     3901 aggatttaaa agtatagttt catctaaaaa atactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttataa tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtacagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatat aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ctgtcctcca ttctatatcc
     6301 tatgcctctt aacgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggactgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca tcatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttt atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcttta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatacttatg taaatataat acttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagctgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatggtgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 atagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 agttttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtaatagtaa
     8701 ttattaaata agataaaaag taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 tacttttgag ttagaaaaaa ctatatttta gagtatgaag agatgaagct tccgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 atgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaacgcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggata tagtgaatta agagttatta tacgcttaaa
     9961 tgaaacagaa gtagaatata aaaatcaaag taaacaccaa ttaatggatt gttataataa
    10021 ggtgatagat gatatagaga agaaggattg ttcgattatg tttgaaagaa tgaaaaaggc
    10081 aaatcgtata atcgtctttg gagaaggtta ttctcaggaa agagttgcta aagagatgaa
    10141 aaggattttc ttaccaacag ggaaaattat atatgatgtt tatggttatg atatgattca
    10201 ggcacttaat cattttataa aaacagatga tatgattttc tttatttcat tgaatggaga
    10261 tgcatctaca attattgatt ttgctaaaga aatgagaatg aaaggaattt atatggtttc
    10321 tattacaaaa atgataagta atcctttagc aagattatgt gatgaaaacc tgtatattca
    10381 atctatatct atttctgtag ataaacaact ggactacgat gtaacaacac cttattttat
    10441 tttaattgaa ctgctttata ttaaatacaa gatgtattta gaaagtgaac atattccccg
    10501 aaaatgaaaa tttgtgctaa aaatgaacat attttttaaa agatatcaaa atattgatat
    10561 cttttttttt gaatgctatt atgattttga tagtaagaaa ggaagatgaa attgtacaaa
    10621 ttattaatac ttgatataga tggtacgctt agagatgagg tatatggaat cccagaatct
    10681 gcaaagcatg ctatacgttt atgtcaaaag aatcattgca gtgttgttat ttgtactgga
    10741 agaagtatgg gtacaataca agatgatgtt ttatcattag gagttgatgg atatattgca
    10801 ggaggaggta attacattca atatcatggt gaattacttt ataatcaatc ttttaatcaa
    10861 agattgataa aggaagttgt ttgtttatta aaaaagagag aagttgcttt ttcgattgag
    10921 agtcaggaaa aagttttcat gaatcaaaaa gccaaagaaa tatttgaaac aatgaatcag
    10981 ttgaaaggaa ctaattcttg tataaataaa caacatatcc aagaaaaaat aacatatgaa
    11041 aataacattg aagagtataa gtcacaagat atacataaaa tttgtttatg gtcaaatgaa
    11101 aaggtatttg acgaagtgaa aaatatacta caagataaaa tggaattagc acagagagat
    11161 atttctagtc agtattatga aattattcaa aaagattttc ataagggcaa agcaattaag
    11221 agattacaag aaagattagg agtaactcaa aaagaaacca tatgttttgg tgatggtcaa
    11281 aacgatgttg tcatgtttca agcatcagac gttacaattg caatgaaaaa tagtcatcaa
    11341 caacttaaag atatagcgac atctatatgt gaggacatat tcgataatgg aatttataaa
    11401 gaattaaaaa gaagaaatat catataaaaa ggagtaattt atggaaaaga aatggtggca
    11461 aaaagaaatc gtttatcaaa tttatccaag aagctttcaa gatagtaata atgatggaat
    11521 tggagattta cagggaatta tttctcgttt agattattta aaaaatttag gtattacatc
    11581 attgtggtta tgtccaattt ttaaatcacc tatggacgat aatgggtatg atattagtga
    11641 ttatttggat attgcaagtg aatttggaag tatggaagat ttaaaagagt taattaaaga
    11701 aagtaaagat agaggaatca aaattatatt agatttagtt cttaatcata caagtgatga
    11761 acatccttgg tttcaagaag cattaaaaaa tccagaaagt ccttatcatg attattatat
    11821 ttttaaaaaa ggaaacgatt taccaaataa ctggaggtct gtttttggtg gaagtgtttg
    11881 ggaaaaggta gagaatagag acgaatatta ttttcattct tttggtaaaa aacaacctga
    11941 ccttaattgg gaaaatccaa tattaagaag tgaactttac caaatgatta atacctggtc
    12001 tcaaatggga gtggcaggat ttagagttga tgctattaca ttcatcaaaa agaatttaag
    12061 ttttcaaagt ttagaaagtg atggtgttga tggtcttgtt aaatgtacaa aaacagttag
    12121 aaatcagcct ggaatagaaa aaatgcttta tgaattaaaa gaaaatacgt ttaaaaaata
    12181 tgattgtatg actgttgctg aagcagcagg tattagctat gaccagctag gtgattttat
    12241 tggagaagat ggtttctttt caatggtctt tgactttaaa catgctgact tagatgttgc
    12301 ttctggtagt gagtggttta aaagaatttc atggacaatt ccagatttaa gaaaatgcat
    12361 tttagatagt caaatgtatg ttcaggaaca tggatgggca gctaatttta ttgaaaatca
    12421 tgaccaacca agagctacaa caaagtatct tttgcaatac gaaaggaatg atgatgcagt
    12481 aaaaatgttg ggagcaatgt atttcttttt aagaggaacc ccatttattt atcagggaca
    12541 ggaattagga atgacaaatt ttaaaagaga taccataaat gattttaatg atttatctag
    12601 cattgaccaa tactatcgtt caatagatga aagttactca gaagaagaag cgttagaatt
    12661 tgtgaattta agaagccgag ataatgcaag aacgccattt ccatgggcag ataaagaaaa
    12721 tgcaggtttt tcagatacaa caccatggtt aaaggttatt gaaaattata aagatattaa
    12781 tgcacagagt cagattaaca attcagagag tattttagaa ttttataaaa agatgataaa
    12841 gttgagacaa gatagtgagt attctgattg tttgatttat ggttcaatag aacctattga
    12901 tatagaaagt gatgatatta ttgcgtatag aaggcaatta gatttggtag ttattgattg
    12961 ttattataat ttttctgata aacctgtaga aataagagta gaagaaaaat atgatattgt
    13021 attccaaaat ttagatgata taaatctatt aaataaaaca gtgcgtttga aaccatttca
    13081 tgcagtatta ttgaaagtga ggtagaaaaa tgaataaata taataaaata gcaaatgagc
    13141 ttattaaaat tattggtgaa gataatatta tttctattac acattgtgcc acaagattaa
    13201 gagtgatggt taaagataga gaaattatta atgataagaa agttgaaaaa gttgatgagg
    13261 ttaaaggggt tttctttact tctggacagt atcaaattat tttaggaaca ggaatagtta
    13321 ataaagttta tgcagaagtt gaaaaaatgg gattaaaaac tttatcaaaa aaagaacaag
    13381 atgaattagt taaaaataat gaaactggat ttaagaaagt tatgagaact ctagcagata
    13441 tatttgttcc tattattcca gttattgcag caacaggatt attcttggga ttaaagggtt
    13501 gtttgtttaa tgataatgta cttggattat ttggaatgtc ttctgcaaat attccattgt
    13561 atattcagac acttgtttcg gttcttacag aaacagcatt tgctttttta ccagcaatta
    13621 ttgtatggtc agcatttaaa gtatttggag gaacacctgt tattggactt gttattggac
    13681 ttatgttagt ttccccaata cttcctaatg catattctgt tgctgaccca tcaaatgaaa
    13741 ttgaagcaat tatggcattt ggatttattc ctattgtagg ttgtcaagga agtgttttaa
    13801 cagctattgt tacagcattt attggagcta atcttgaaaa atggttccgt aaacatatgc
    13861 ctaatgtatt agaccttatt ttcacaccat tttttgttat gctaataaca atgttagtta
    13921 ttttgttagg tgtaggccct ataatgcata caatagaatt aaaaatggtt gatatcatca
    13981 gcttattgat tgatttgcct ttaggaattg gaggatttat tattggattt acatatccat
    14041 tagctgttat tactggctta catcatactt atgtaatgat tgaaacatca ctattagcaa
    14101 atacaggatt taatgccctt attactcttt gtgcaatgta tggatttgcg aatattggaa
    14161 catgtctagc atttatgaaa aaatctaaaa ataatcaagt taaacaaact gcggttggag
    14221 caatgctatc tcaacttttt ggtataagtg agcctgtttt atttgggatt cagcttcgat
    14281 ataatttaaa gcctttgatt attatgtgtg catcttcagg tttaggagca gcaatattat
    14341 caattttaca tattcaatct aattcatatg gattagcagt tttaccttct tatttaatgt
    14401 atatctatga tggatataat ttaatcacat acttattagt atcaatattt gtggttgcat
    14461 tctgctttat tgtaacatgt ttatttggag tgcctaaaga agctataaat gaagatgaag
    14521 acgaagaatt agtgtttaat gaaaataatg aaaactttgt gtctccagct aaggggaaaa
    14581 ttgttgcatt agaaaatgta ccagatgaaa cattttctaa aaaaatgtta ggtgatggtt
    14641 ttgctattga tattatagat ggaaaaattg tttctcctat ttcaggtaaa ttagaaacag
    14701 tggtttcaag tggacatgca tttgggatta aaggaactaa tggagaagta ttaattcatg
    14761 ttggaattga tacagttgca ttaaatggag atggctttga tgttgctgtt aaacaaggag
    14821 atatggttaa acagggagac gttcttgtta atgttgattt aaagagaata catgaattag
    14881 gaaagtctac tttgacaatg gtattgtttc cagatggtaa gaaggtaaat attttggata
    14941 taaacaagga tgtaaaaata ggtcaaagaa tatgtgttga gtagtaggtg atatgtaatg
    15001 actacaaaag aagattatca agagtaattc aatcttttta aagtatgaga aataattcat
    15061 aaactctaaa acaattaaag aggttgtttc aaaatagaga ttgtattgca cccaaattgt
    15121 tgaaatgatg aaaatatatt taacttcata caaaaaatta aatagtggca acaattgagg
    15181 atgtgaggga gagttgcca
//
LOCUS       DAMKMI010000337        11968 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.guided.122, whole genome shotgun sequence.
ACCESSION   DAMKMI010000337 DAMKMI010000000
VERSION     DAMKMI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11968
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.guided.122"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            156..1562
                     /locus_tag="PC429_003932"
     CDS             156..1562
                     /locus_tag="PC429_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF3849325.1"
                     /translation="MNTGFPKEFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1590..2438
                     /locus_tag="PC429_003933"
     CDS             1590..2438
                     /locus_tag="PC429_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF3849326.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            3099..3791
                     /locus_tag="PC429_003934"
     CDS             3099..3791
                     /locus_tag="PC429_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF3849327.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3788..4723
                     /locus_tag="PC429_003935"
     CDS             3788..4723
                     /locus_tag="PC429_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF3849328.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIYKRLKDIEEIL
                     ADVQIGDENRKILIKPYDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDRDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQISVESEIGKYTIFTVSLPLI"
     gene            4871..5794
                     /locus_tag="PC429_003936"
     CDS             4871..5794
                     /locus_tag="PC429_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF3849329.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5778..6548
                     /locus_tag="PC429_003937"
     CDS             5778..6548
                     /locus_tag="PC429_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3849330.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVQKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPILSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            6558..7340
                     /locus_tag="PC429_003938"
     CDS             6558..7340
                     /locus_tag="PC429_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF3849331.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVTLVVKSLVQILSINLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7717..8253
                     /locus_tag="PC429_003939"
     CDS             7717..8253
                     /locus_tag="PC429_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF3849332.1"
                     /translation="MNLKGTKTEKNLIAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8285..8674
                     /locus_tag="PC429_003940"
     CDS             8285..8674
                     /locus_tag="PC429_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF3849333.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8711..9097
                     /locus_tag="PC429_003941"
     CDS             8711..9097
                     /locus_tag="PC429_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF3849334.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPITKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9224..10666
                     /locus_tag="PC429_003942"
     CDS             9224..10666
                     /locus_tag="PC429_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF3849335.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEVSNYTNIQHV"
     gene            10963..11778
                     /locus_tag="PC429_003943"
     CDS             10963..11778
                     /locus_tag="PC429_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF3849336.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         4316 a         1484 c         2262 g         3906 t
ORIGIN      
        1 aatatagagt aagtttattg taatgaattg ttaaagaaaa taattaatat agggtaaatc
       61 tatctcaatg aaataacaaa gaaaagagtt aatatagagt gaacttattg caatgaatca
      121 acaaagaaaa taattaataa aaaggagaaa tatttatgaa tacaggattt ccaaaagaat
      181 ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat aaagacaata
      241 aaggaaaaac cgtagccgac tataattctt ttaaaaagtc tcatttacag gcagatacta
      301 aagtcgcatc agacttttac cataattatg aagaagatat tgaattaatg aaagaattag
      361 ggatgaaaac atatcgtttt tcaatttcat gggcaagaat aataccagat ggtgaaggag
      421 agataaacca gaaaggttta gacttttata ataagataat agacaaacta atagagtgtg
      481 atattgagcc attcgtaact ttataccatt ttgaccttcc atttaaatta gtagagaaat
      541 ataatggttg ggaaagtaga gaaactgtct atgcttttga aagatttgct aaaatatgct
      601 ttaagcattt tggagatagg gtaaagtatt ggcaacctca taatgaacag aatttaattg
      661 ttagagtgga ggagagaatt aatatatatg atgaaacaga ttcatggaag atagacaaaa
      721 tgcgtgctca gatggattat aatctttgct tagcacatgc attagcagtc aatgcatgtc
      781 atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta acatatcctc
      841 ttacttctaa gccagaagat gtatatgcag ctagaatgaa tgataatttt aaagtgtact
      901 atatgcttga tatgcatcat tatggagaat atccagggta ttatatgaag tatcttgaaa
      961 aaagaaatat agttcctcac atggaagatg gggataaaga gatacttaaa aaagctaaaa
     1021 tggattttat agctgtaaat tattatagga caaattgtgc agaagcttta cctgaagatt
     1081 cacaacatcc atttggatta agagaaggaa ctgttgattt tagtatgtat ggactattta
     1141 aaatgtcaat gaatcctaac ttagaggctt ctgagtatgg agcagcaata gacccatcag
     1201 gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt ataataacag
     1261 aaaatggtct aggagctaaa gatatattag aagatggaaa aattcatgat gactatcgta
     1321 tagactattt aagaagtcat ataaatgcat gtaaactagc tattgaagat ggtgtagaga
     1381 tgattggata ttgtccatgg tcatttacag accttttaag ttcttctcaa ggttttaata
     1441 agcgttatgg acttgtttat ataaatagaa ctgaccatga aattttagat ttaaaaagaa
     1501 tcaagaaaga tagtttttac tggtataaag aagttataga gaacaatgga atagtaaaat
     1561 aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta aataattcgt
     1621 tgctacttgc aaaggatgat aatgatgaag aaataatatt gatggggaaa ggcattggcc
     1681 ataattataa ggccgggtct gagttaaata aaaaagatat agaaaagatt tttgttcttc
     1741 atgatgaaaa tataaaaaag agtattatac aattggcaac agaaatagat gcagaatatt
     1801 ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta aaacttatga
     1861 accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgt tttaaagaag
     1921 gtataatgat tgaaaatcac tatttgttcg aaatcaagga atttaatcca aaagagtatg
     1981 atataggaaa atatgctatt actgttttta aggaggttct tggtttagac ctaccagaag
     2041 aagagattgg taatatagca ggccatttta taaatgcaca acaagataat ccttatagtg
     2101 atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt cattatcact
     2161 tttctattgt atataataaa gagtcatttt attataggag atttgtaatg catctaaagg
     2221 ccttttcaca aagatttctg gctaatgaac catcaagaga aaaaatagat tttatatatg
     2281 agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta ggagcttata
     2341 tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg atgattcata
     2401 ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata tgctagtaat
     2461 attataaaaa attaaaaatt aactacactt caaatatgaa taattgcatt acttaagggg
     2521 gttgtcttaa aattgatttt tttaattaaa tttgaagctc aactcctttt ttaaaccttt
     2581 atatcattac aactttaatg tttatacgcc tgtacttcag atgtatatct actttttaca
     2641 tttctcaaca tgcaaatcta ccattttttg cagtatgtta attgaaataa acctttgagt
     2701 taaccctaaa tttattctgg acttatagga atagttccaa agatacttta aaatattttc
     2761 tggaatgatt gcttatttca catatgtttt attgtgatgt tttgatttaa taaattcttc
     2821 aactctgtat atagtaaatg tattatcatg tttgttttat tcaagcacac ttataatacg
     2881 cttgattttt tcacaatgat gaaatatact aatgggtgtt ataagtataa gaatagcatt
     2941 acacttgaag aagcaagtga taaatgaggt ataaatcctc atatgataaa ctactatttc
     3001 attgaggagc atattctaag tgatgtcaag atactacaat atgactaaat tcctagtgat
     3061 aaaaaagtct acgaatgagt gtagaaaggg gaactaagat gaataaagtt ttaattatag
     3121 atgatgataa agatctttgt attttgttaa aaaagagtat ctcaattgaa cagattgaag
     3181 caaactattg ccattctggg agagatggac tggttgaact ttcaaaacaa gattatcagt
     3241 tagtagttct agacgtgatg atgccaggta tggatggttt tgaaactatg gaacaaatac
     3301 gaaaatatag cagtattccc atcttaatgc tgacatcaaa aagcgataat tacagcaaag
     3361 tgcatggatt gcgagctggt gcagatgact atctaaccaa accatttgaa attacagaat
     3421 ttactgctcg tgtactgtct ttaatccgac gttacacccg ttttaatcca aaaaatgagg
     3481 aaccacagca actttctttc aaaggtttgt tgattgacct tgaccagcat acagtgacta
     3541 ccaatggcga acctatgttg cttccaccca aggaatttgg tgtacttctt tactgtgcaa
     3601 ggcatcaggg taagattttg acaaaacagc agatttatga agccgtgtgg ggtgagccat
     3661 atgtatatga tgacagcaat atcatggcta ttatcagtcg cctaaggaaa aaaattgaac
     3721 tagacccatc atctcccatc tatttgcaaa caataaaagg aattgggtat caattcaaca
     3781 cggaggtatg aaaatggatg aacaaacgat aattcttttc ttactcttgc tgtcgattgg
     3841 tctacttttt ctttccatat ttgctttttc caagcttggg catatttaca agcgcctaaa
     3901 agatatagag gagatactgg ctgatgtaca aattggagac gaaaatcgta aaatactaat
     3961 taagccatat gatgttatgg ctccattggt ttatcagctt aatgagattg tctacgatta
     4021 tgaaaataag ttactttctc tgaaaaagtc ggataaagct agcaaacaac ttatgacaag
     4081 tttatctcat gatgttcgta caccattaac aactctgatt ggttatctgg atgctgtaca
     4141 cagtggtata gttatcgaac aagagcggga ggaatacctt gaaatcgcta ggcgtagagc
     4201 ctacgacctc aaggattaca ttgatgttct ttttgactgg tttcggttga actctgatga
     4261 atttacgctg tcaatagaat ctgtggaaat tgcagagttg tcacgcaata ttttgaaaga
     4321 ctggattcct atattccatg aaaagaaact gaattttgaa attgatattc cagaaaatcg
     4381 actaatggta aacctcgacc gagatggata ttcccgtgtg gtcaataatc tggttcaaaa
     4441 tgttcttgct cacagtaagg ctcggcaaat caaaattacc atgagtgagg attccagaat
     4501 ggtattactc cgtgttgagg atgatggtgt tgggattgcc agagaaaatt taccacatat
     4561 ttttgagcga ctgtataagt gtgataaagg acgttcagaa aaaggaagtg gtcttggtct
     4621 atctattgtc agtcaaatgg tggagcgaat gggtggtcag atttccgtag aaagtgagat
     4681 tggaaagtat accatattta cagtgagctt accattaata taaaattgtt tttcccccat
     4741 gtgacatgta tacacagggg gattttttgt aagttataaa gttgcaaggt taatacaagg
     4801 ttgttgcaag gttaatgtaa gattgaggtg gtatgataac tttaataaac aaaggaggct
     4861 tttattattt atgaataatt taattattga aacaaaaaat ctcactaagc aatatggtgc
     4921 tcaaaagagc gtttctaatt tgaatattca cgttcagaaa ggtcgcattt acggattgct
     4981 aggaagaaac ggtgcgggta agacgacaac tatgaaaatg ttattgggtc taacaaaacc
     5041 aactactgga gaaattaaaa tatttggtca ggacattcac aataacgaga agaagcttct
     5101 tcctcgaatt ggatgtttga ttgaatcacc agggttttat cctaatctga caggaacaga
     5161 aaatctcaaa atctttgcaa ggcttagagg aataccaaaa aaaaatgcag tagaatcagc
     5221 actggatgta gtcggtttgc caaacaatga caaaaagtta ttttctcaat actctcttgg
     5281 tatgaaacag aggttggcga ttgctcttgc cattatgcat gacccacaac tgttgatttt
     5341 ggacgagcct atcaatggac ttgatcctat tggtattgca gaagtaagaa cgtttattcg
     5401 tgatctctgc acagaacgag gtaaaactat tttgatttcc agtcacattc tttctgaaat
     5461 tgccttgttg gcagatgaca ttggcattat tgaccatggt attttgttgg aggaagaaag
     5521 tcttgcccaa ttagaagaaa aaaacagtaa atacatacat tttgctgtat ctgatgcagc
     5581 acaggcagca aggcttctgg aaacccaact taacctgaaa aatttcaaag tagcagataa
     5641 ttacaatatt atgctatatg atacagatgt ttctgttgca gaaataaatc gtgctttcat
     5701 tctaaacaac attgaggtat cagaatctca tttatgcgaa gatactcttg aagattactt
     5761 caagaaagta acagggggtg aaggaattgc ttgatataat ttcatgtgaa ttttcaaaac
     5821 tcaagcggtt aaaatttata ctgatttcta ttttaggtgc ttgcttattt ccaattcccg
     5881 caacaatttt gattgcaaag gacaacttgc catttgagca attgttcaaa ttagttgtca
     5941 attttggata ttttttgcta ttaccaattg tattaagtat tgttgctagc caactatttt
     6001 ttatagaaag agataatgat gtattaaaaa atcttgcaac agtgccagtg caaaaaggaa
     6061 aactggctct ggcaaaattg gctgtgttgc tgtttatatc attgttctac tctgttgcag
     6121 ggttaggagc tactatcatt ggaggtttca ttgttggaat agtagagggg gttgctgtaa
     6181 aattaggaat gagcattgct tttggaatta tgttttttgt tactgtactt cctgttgtgg
     6241 tactcattgt atatttcaat cgcagctata ttttttctat tatactttct tttgtatatg
     6301 ctattttcaa ttttagtata tccttaaata ttatcaattt tgaaccaaat aacccaatat
     6361 taagtgtatt acctgctcct gttataatgc gttggtggat ggcattttgg ggagatccca
     6421 ctggcgaata cacggcactt agacagccat atttgctctc aactcctgct tgtgtaggta
     6481 ttttatgttt gataaccatt atagcagtcc tgctgatttg tatgatatac aaaaagcagg
     6541 agaattagga ggtatctatg cttgatataa ttactacaga atttcaaaaa ataaaaagat
     6601 ataatattct atggattggt gttgttgctg ttctgttttc agcactattg gcagtttttc
     6661 aggttgtaag ttcacacggc agtgaccctc taacgtatga gagctttgca aacggagtta
     6721 tctggaataa tttcagtctt agttttccat ttgggattac acttattgga ggatatctta
     6781 ttaaccgtga atatacagac caaaccttaa aaaacatcct aactgttcct atttcattac
     6841 gcaaattgct agtgggaaaa ttaattgcgg ttggaggaat tactgtattg ttaagtcttt
     6901 tcagcttctt atgcacattt attttggggt tgttattttg taatatcaat atatctgtta
     6961 cactcgttgt aaaatcctta gttcagattt tgtctattaa tctgtattgt tatgttgctg
     7021 ttttgccgat tattgcttat tttggtcgta aacaaaatgc atttctgacg ggtgtaggga
     7081 ttgcatttgt atacggtttt tgtggtgtgt ttgttgctgg aagaaatctg acagactttt
     7141 atccaatcac agcaggtctt ggtctagttg gatacaacaa tggtgctggt actgtttatc
     7201 agccacttat cggtgctatg actttgattg taattctaat cttgacaact attctacttg
     7261 tatttacacc taattatgat aaagttatga ccatatctaa gaaaaaaggg ataaagcata
     7321 aaaagtattc tcataaataa cataaatatt gtttctatta tttaagttgc tagacagtga
     7381 aaagaaaaaa tctgtcgtac agtggctata tgctcatgta ttcaagaagc tacagttttg
     7441 atttttcata actgtagctt ctttttgtat ttattaaatc acgactatca taatacttgc
     7501 aataggtata gcgacaagag agagtactgt catatgtgtt ttatatatta cattaagtat
     7561 attctaaaat atatgtttta aataatatat ttgaaaatag gaatatttaa ataaaaaaaa
     7621 taatattata tatagttgac aaaaattggc aaatgatata atatataaat atagaaatag
     7681 taatcattac tattattaaa aagggaggaa ttaattatga acttaaaagg aactaaaaca
     7741 gaaaagaatt taattgcagg atttgcagga gagtcagaag caagaaataa atatacatac
     7801 tacgcttcaa aagcaagaaa agaaggttat aatcaaatag ctgctatatt tgaagaaact
     7861 gctaacaatg aaaaggaaca tgctaaatta tggtttaaac ttttacatga tggtatgcct
     7921 tcaacagagg aaaatttaaa ggatgcagca gcaggtgaaa actatgaatg gactgatatg
     7981 tatgcaaaat ttgctaaaga agctagagaa gaaggatttg acaagatagc ttatttattt
     8041 gaagctgttg gaaaaataga gaaagagcat gaagaaagat acttaaagct tttagaaaac
     8101 ttaaatgaag gaaaaatatt taaaagagat gaagaagttg tttggcaatg tcaaaactgt
     8161 ggtcatgtgt atgtaggtac tgaagcacca gaaaaatgcc cagtatgtga ccatccaaaa
     8221 gcatatttta atataaaagc tgaaaattat taaaaattat cattattaaa aaggaatgtt
     8281 aactatgaaa ttttctaaac aacgagaact gattttaaat gaaatattaa ataatccggt
     8341 tcatcctact gcggattacc tatatgaaaa cttaaaaaaa gataatccaa atttaagctt
     8401 aggaactgtg tatagaaatt tagctcagct aacggaacat ggctttataa gaaaggttag
     8461 tattccaggg tatccagata gatttgatgg tcgaatagat aatcactatc atatcatatg
     8521 tgaagtatgt ggagaggtat acgatttaga atccgaagtt cttaataatt tacaagaatt
     8581 aatatctgag gagacagaca taaaaataac atcttataac ataagtttta aagggatttg
     8641 taataattgt aaaaggtgta gccaagtagg ttaattttca aattctaaaa ataattaatg
     8701 aggtgagttt atgtgtagtg aacaaaaatt ttttatatgt gagacttgtg gaaaccttgt
     8761 aggaatgata caaagtggtg gtgtacctat tttctgttgt ggaaaaccta tgaaggaact
     8821 tgttcctaat acaacagatg cagcagttga aaaacatgta cctgtaattg aagtggatgg
     8881 aaataatgtt actgttaaag taagtagcac aactcatcca ataactaaag aacatcatat
     8941 agcatgggtg tatcttatga ctgaacaagg tggtcaacgc aaatgtctag cagtagatgg
     9001 agaacctgta gttaaatttg ctttgaatga tgatgataaa gtgatttctg cctatgctta
     9061 ctgtaattta catggattat ggaaggctga actgtaatat ttaaataaat tgagtcaaaa
     9121 aggaggctat aaatgtattt tcatacaaat agcctcctat aaatttatat aaattcaagt
     9181 tttaattttg tttaatgtca atataatata aggagaaaat aatatgtcaa tttataaatg
     9241 tagtgtttgt ggatatattt atgatgaatc taagaatgat aaaacatggg atgaattaag
     9301 tgaagattgg gaatgccctg tatgtacaaa aggtcgtagt tattttggaa agataagtac
     9361 tgtttactat gaagaagatg aaaagatagc agaagacata gttgaagatg aatctaaatt
     9421 aaatactgaa aaagagggag atttaaatta cttaagtaca tatttaagaa gagatgatga
     9481 agttgagaaa catatggata taattcacga aatggctgtt acaggaaaat ctataataga
     9541 acctatgaga actaaactac cagtaatttc ttgggatgat attttaatta tgggtgctca
     9601 attaaatcca ttacctttaa atgagcatga tgaagtaaat acaacaacaa taataggaaa
     9661 aaaagcaaag aaacctatga ttatagaaaa tccagtttat atttcccaca tgtcatttgg
     9721 agctttgtct aaagaactaa aaatagcttt agcaaagggg gcagcccaaa ataagacagc
     9781 tatgtgtagt ggtgaaggag gtatattacc agaagagaag gaagcttcat acaagtatat
     9841 ttttgagtat gtaccaaata aatatagtgt aactgaagaa aatcttaaaa attctgatgc
     9901 tatagagata aaaattggtc aaggaacaaa acctggtatg ggaggtcatc ttcctggcga
     9961 aaaagtaact gaagaaatag caaaagtaag aaataagcct gtaggtcaag atgtaataag
    10021 tccatcttgt tttgaggaaa tacaatccaa agaagattta aagaaacttg tagatgaact
    10081 tagagaagtt tcagaagggc gtccaatagg tgttaaaatt tcagcaggtc atattgaaaa
    10141 agatatggaa tttatagctt atgctaaacc tgattttgta actattgatg gtcgtggtgg
    10201 agctactgga gcaagtccaa agttgcttaa agatgcaact tcaattccaa caatatttgc
    10261 tctttatcgt gctagaaaat acatagatac acatggatta gatatagatt tggttataac
    10321 aggtgggctt cgtatatcta ctgattttgc taaggcaatt gctatggggg ctgatgcagt
    10381 tgctattgca agttctgctc ttatggcagc ggcgtgtcaa caatatcgta tatgtggttc
    10441 tggtaagtgt cctgtaggag tagctactca ggatgaggaa ttgagaaaaa gattacatat
    10501 agaaaatagt gctaatagag tggcaaactt tttaaatgta agtttagaag aacttaagac
    10561 ttttgctaga atttctggtc ataaagatat acatgattta agtgttgatg atttatatac
    10621 tgttaactct gaagtatcaa attatacaaa catacagcat gtatagattg aagtttgaaa
    10681 ctattagatt ttagatagct ataaaagtaa atgataaata tattttataa aagagagtgt
    10741 cttaaaatga actttttagt tcataagtca ctcttttttg tatgaagcca aatatattta
    10801 tcattttagc aatgaaaaag aatcttatct ctttattttg agatagactc cttctattta
    10861 tgaaaattaa agatattaaa ttgtaaaata ttctgtgatg tcgtactatt aaactaaaga
    10921 ttctatactt taaagtaaat aaaaatttaa ggagtgatta ttatggaaag attgaagata
    10981 caagttttag tcgacaataa tacttatatt gatagatact ttgttggaga accagcagta
    11041 agctattata ttgagattga tggaaatagg attttgtttg actcagggta ttcagatgta
    11101 ttcatatcaa acgctgaaaa attaaatata gatttaggaa atttaactca tgttgttttt
    11161 tctcatgggc ataatgacca tacaagaggt atccagtttt tagaaaatag gtatgattta
    11221 tctacagttg aacttatttc acatcctaac tgttttatac ctagaaaaca tggagagaaa
    11281 agcataggag ctccattttc tgctgaggaa attaaaaata tttttatgta taatcctaag
    11341 gataagccat ttaatctaag caagaattgt gtgtttttag gtgaaatacc ttctataaat
    11401 gattttgaaa aaagagctaa aattggtaaa tgtaaaatag gagatttgtg ggaagatgat
    11461 tatgttttag atgattctgc tatagtttgt aaaactgata aaggtatttt tattgttaca
    11521 ggatgttctc atagtggaat ttgtaatata gtagagtatg ctaaaaaggt atgtggagat
    11581 gatagagtta ttggtatact aggtggtttt catctttttg aattgaataa taggttagat
    11641 agtacaatac aatatttgga taaagaagaa ataagaatgt tgtacccttg tcactgtgtt
    11701 tcttttaagg taaaagcgaa gatgaatgaa atattgaata taaatgaagt tggagtaggg
    11761 ctgaccataa gtatatagta tttgaaagta tataagattt aagttgaagg ttaaaaagat
    11821 attttctata gaaaatatct tttttgttac gtttatttta agagaaatca atatactttt
    11881 atatgaaata ttgtaaaaaa atttattttt aatgagaata tattttcatt aataaacaat
    11941 tttattatgt attaataatg ttagaata
//
LOCUS       DAMKMI010000338        11195 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain L,15.7104142 isolate JH430
            SAMN32366243-rid16589313.guided.132, whole genome shotgun sequence.
ACCESSION   DAMKMI010000338 DAMKMI010000000
VERSION     DAMKMI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366243
            Sequence Read Archive: SRR22862852
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560708.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560708.1
            Genome Coverage       :: 268x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:12:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,958
            CDSs (total)                      :: 3,872
            Genes (coding)                    :: 3,818
            CDSs (with protein)               :: 3,818
            Genes (RNA)                       :: 86
            rRNAs                             :: 7, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 21 of 54
            Pseudo Genes (incomplete)         :: 31 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 9 of 54
            CRISPR Arrays                     :: 9
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366243-rid16589313.guided.132"
                     /strain="L,15.7104142"
                     /isolate="JH430"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="Ireland"
                     /collection_date="2012"
     gene            159..497
                     /locus_tag="PC429_003944"
     CDS             159..497
                     /locus_tag="PC429_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3849337.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            519..836
                     /locus_tag="PC429_003945"
     CDS             519..836
                     /locus_tag="PC429_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF3849338.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            986..1663
                     /locus_tag="PC429_003946"
     CDS             986..1663
                     /locus_tag="PC429_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849339.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(1991..2758)
                     /locus_tag="PC429_003947"
     CDS             complement(1991..2758)
                     /locus_tag="PC429_003947"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF3849340.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(3430..4245)
                     /locus_tag="PC429_003948"
     CDS             complement(3430..4245)
                     /locus_tag="PC429_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDF3849341.1"
                     /translation="MVKLSDAKSWAIRMSEDGCAYKSIDNYKRSLKASFFMAIQDDCI
                     RKNPFNFQLSDVLEDTTEPKIALTPEQEMGLLSFMESDKTYCKYYDDVVILLETGLRI
                     SELCGLTLELDMKNKAIMVDHQLLSDTKIGYYVEVPKTTQGKREIPMTERAYQSFERI
                     VERRKNAKAEPIIVDGYQNFLFLKQDGLPKQATHYQSMLRALVKKYNKTHEDKLPKIT
                     PHTFRHTLCTNMANKGMSPNILQYIMGHKNITMTLGYYAHGSFSSSRAEMERL"
     gene            4337..5605
                     /locus_tag="PC429_003949"
                     /pseudo
     CDS             4337..5605
                     /locus_tag="PC429_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            5704..6324
                     /locus_tag="PC429_003950"
     CDS             5704..6324
                     /locus_tag="PC429_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF3849342.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            6474..6878
                     /locus_tag="PC429_003951"
                     /pseudo
     CDS             6474..6878
                     /locus_tag="PC429_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
     gene            7003..7929
                     /locus_tag="PC429_003952"
     CDS             7003..7929
                     /locus_tag="PC429_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF3849343.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            7943..8392
                     /locus_tag="PC429_003953"
     CDS             7943..8392
                     /locus_tag="PC429_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF3849344.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            8752..9159
                     /locus_tag="PC429_003954"
     CDS             8752..9159
                     /locus_tag="PC429_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF3849345.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            9161..9391
                     /locus_tag="PC429_003955"
     CDS             9161..9391
                     /locus_tag="PC429_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF3849346.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            9781..9984
                     /locus_tag="PC429_003956"
     CDS             9781..9984
                     /locus_tag="PC429_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF3849347.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            10062..10415
                     /locus_tag="PC429_003957"
     CDS             10062..10415
                     /locus_tag="PC429_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF3849348.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            10487..11119
                     /locus_tag="PC429_003958"
     CDS             10487..11119
                     /locus_tag="PC429_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF3849349.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         4093 a         1618 c         1779 g         3705 t
ORIGIN      
        1 atccatattt gtttattgta gatacagact atacaaaaat aaatagcact tatttcagta
       61 taaaaacata tatagtctat atttagacta aaaaagtata aatagtctat ataaaacaac
      121 ttaaactatg aatataatct aaagtggagg gataataatt ggcaaatata ggaaaaataa
      181 taggagatag aataaaagtc tatagaacaa aattgggata tagtcaagag tttctagcag
      241 aaaaggcagg tttacatcca acatatattg gtcagattga aagagctgag acaaatacat
      301 ctatcaatat tataatgaaa attgctactg ctttagacat accacttgaa ttgttatttg
      361 caaatattat tacaacagaa ggcgttgata actctgtacc tttagaatgt tatgaaatga
      421 ttaataaatt aactgaaaaa gaacaaattg caatgctaga gttaataaag tgcatcatta
      481 aatacaaaga aatataagtg agggaaatag taacaataat gaattatata ggtaaaagac
      541 ttaaagaaga acggaaaaaa gcaaatttaa cttcaaaaga atttgcaaat atggttggag
      601 tatcgccttg gtatataact caaatcgaaa gtggtaaaaa gaatccaagt cttaaaacat
      661 tcataaaatt tgtaaatata ttaaatattt ctgcagatgt tcttataaaa gatattactt
      721 ccactggaaa aacatattta gaaaatgata taaatgaaga gttaaaagac ttaaattcaa
      781 gagaactaaa tctgatatca cagattatag acatcataaa aaagaataaa atataattct
      841 ttatatttta tataaaaaaa aaggttattt gaaataaata accttttttt gtctttttgt
      901 gttaacattg tcttatagtg ctaatgagta ctattagacc atgttctatg gccttatttt
      961 aatataaaac gattggagat aggctatgat tgtgattgaa ggtagcgata aatttaagat
     1021 tgcaaaagaa tatattgatg tagaatatac tctttttagc aaagtaactt ttaggtatga
     1081 aaagttgaaa tttaaagata atgctgaatt ggaaaaaatt aagatgttta aatataaaaa
     1141 tggctacatc cctaataaat taaacctttc ttttggatat ggattctctt cttataaaaa
     1201 gcaaataatt agagaaactg tagatacttt aagattgaca gaaatttttt caagcgagaa
     1261 catagaagat attaaattta taaaagatgg tacaaaaaaa ttagaaatta gcatagagaa
     1321 agttgtgaaa tttaaacgtc gaaaaaaaag aaattatgtt tgttgctatt gccctgatat
     1381 gtatagagac ataaaactcg acaaagaatc tatcaataag atatacaaca gaaaaataaa
     1441 aatagaaaga gaagttaata tttttgaaga tgaggatgtt ataataaaca agaaagtatt
     1501 gaagtttcca aagtcttgga caaagaatat gcaaaaatat tggttaagtg aaaataagta
     1561 tcccatacat tctactgtaa ttgatgatga tagatataaa tgttgtaatg tacaatatac
     1621 aaaaaataga gtgataatat tatattacat atataaccat taatatacat aattacaaaa
     1681 aaataatgac aaatatacat aatacaaata tatcttctac tttagataaa acagttatta
     1741 tgaaaactta ctatctataa ataatagaaa tgctagtata ccatctataa tctaatgtct
     1801 agtaggtttt aatggcatat tttaaatatg ccatagttga tttataagat tttctatatc
     1861 actttcaaaa tacacactaa atagttgctt ttcagatata attgcccaat ttaaatttta
     1921 ctatctgaaa agttttttat atttaattta tatcttcaaa aataccgtct aatatttcta
     1981 tataattaat ttaattaata ctcttatgta atagattaag tactgaattt aactctttaa
     2041 atgatatttg ccctggtgct gaaacacttt ttgcagctcc aaaagtcaat gcagaaccaa
     2101 atatttcacc acacaatcta cttataacac ccattcctga cattgacatt gttattattg
     2161 gtctatctgc ataaatttta aacatttcat ttgtagcttc aagtagcact aacacatctt
     2221 tttcattttg cggcataaca gctattttgg gtaaatctgc gcccaattcc tgcattctac
     2281 ataaacgaga tacaatctct tctttttttg gagttttatt aaaatcatga ttagatataa
     2341 ttactttcac ttctttttta tgagcaaaat taactacttc atctatgacc tcatctccca
     2401 taaataactc tacatctatt aaatctacta aacctgtatt agaaatttct ttattcaaag
     2461 tagtataata atctcttgat attaattttt ctcccccttc tactacactt ctaaatgtga
     2521 ataataaagg gatatcatgt atataacttc ttaattcata gagaacttcc ttaacttctt
     2581 taatattttc tacattctca aaaaaatcaa ctcgccactc tataatatcc aaacatgcat
     2641 cttttaactc tttagcctct tttataatat cttttttatt tttcccaata attggtacac
     2701 aaattttagg tctaccttct ccaatagtaa tattttttac ttgaactttt cttttcatat
     2761 tctcctccat aatcagaaat tctataggac tttaaattct attttctgca caatttaatt
     2821 atacattaaa aatactatac attataaaaa tactaattta aaaataattt ttattctaat
     2881 tcttaaaaca atcccacata ttttagaaaa ggtactctat aatatacaaa cacttctttt
     2941 ttacctaaat ttattttaac atcctataat tttcacaata ttctttaaat tttactcata
     3001 taaacaatat tattctggat gctaatagaa ttaatctata aatcatagta taattatgca
     3061 gaaataactc gaatttcact acttatgaag agattgaata ttaatgaatc taaatcattt
     3121 gcattactta agagttttga ctaaagttga acaccataca aaagtagcta ctcaattgtt
     3181 tataccccaa ccaagcttaa gtaatgttat atagattctc gaaaaagaat ttggtatctt
     3241 tttatttgaa aaacttatat taaatttgat tatcttttca taagttggta aatgtcgata
     3301 tatcctgtat ctacaaggag tatcggcatt ttctttttgg aagatacttt gcataagttg
     3361 gcatttaacc gtataagaat gccatcaaaa gtagtaaata agtagtaaac tctagtcata
     3421 ctactaggat tataaccgtt ccatttcagc tctcgaagaa ctaaatgaac catgtgcata
     3481 atagccaagc gtcatagtaa tgtttttatg ccccataata tattgcaata tattaggact
     3541 cattcctttg ttagccatat ttgtacagag tgtatgccta aaggtatgtg gcgtaatctt
     3601 tggtagcttg tcctcatggg tcttattata cttcttgact aacgctctta acatgctttg
     3661 ataatgagtt gcttgttttg gtaatccatc ttgtttaaga aataggaagt tctggtaacc
     3721 atcaacaatg attggttctg ctttagcgtt cttacgccgt tccacgatac gttcaaatga
     3781 ttgataagct ctttcagtca ttggaatttc tcgcttaccc tgtgtggtct ttggaacttc
     3841 tacataatag ccaatcttag tatcactcaa caattgatgg tctaccataa tggctttatt
     3901 tttcatatcc aattctaaag tcaaaccgca taattcagaa atgcgtagtc ctgtttctaa
     3961 gaggataaca acatcatcat aatatttaca ataagtctta tcgctctcca taaaggataa
     4021 taagcccatt tcctgttctg gtgttagagc gatttttggt tctgttgtat cttccaaaac
     4081 atcacttaac tgaaaattaa atggattctt tctaatacag tcgtcttgaa ttgccatgaa
     4141 aaacgacgct ttcagtgagc gcttataatt gtcaatggat ttataagcac acccatcttc
     4201 actcattcgg atagcccagc ttttagcgtc tgataattta accatatcaa tgctttttgc
     4261 acctagtggg tcattgatag tatctatatt ttgatttata atttatacat ttttttaaaa
     4321 acagactttt aactgtaaag aatctatgta taaacttagc tatgttgtcc gtgtatctac
     4381 aaaggatctt gacgagctaa aaaggcgttg tgatgaagtc aaagactttt acgacgactt
     4441 atcactcaag ctcgtgcgtc cctttgggga tatgttggga ttgcatggcg aatttattcc
     4501 tgtcagcaaa cgttacatca atgattatat tcaatatgtc acttcggatt ttttggctgg
     4561 acttggtttt ggagcgacac aaacccttgg cgaacatgag gatatctatc ttggctacaa
     4621 tctggacact ggaaagaatg tctatctaaa acctgccctt gctagtcaat gggtaaaggg
     4681 ttctattacc aatgtgttag cgtctgcgtt tatcggttca ttgggtggtg gtaaattatt
     4741 cagtaacaat atgcttgttt actatgcggt actattcggc ggacaggcgg tcatagttga
     4801 ccctaaagca gaacgcggca aatggaaaga aacgctacca gaaatagccc atgagataaa
     4861 cattgtcaat ttaaccagta atgatgaaaa caaaggattg cttgaccctt acgtcatttt
     4921 gagccgtaaa aatgattctg aaagtttagc gattgatatc ctaacattcc ttgctggtat
     4981 ctccagtcgt gacagtgata agttcccagt gcttagaaaa gcaatccgca atgtaaccca
     5041 aagtgaacag cgagggttat tacttgtcat tgatgaatta cgaaaagaag atacttctat
     5101 cagtaacagc atagccgacc atatcgaaag ttttgtagac tatgattttg cacatctact
     5161 gtttggtgac ggaaatgtga agcagtctat cagcttggag aaacagctga atattattca
     5221 agttgctgac ctcgtattgc cagatgccaa cactagcttt gaagaatata caacaataga
     5281 acttcttagt gttgctatgt tgattgttat tagtaccttt gcattagact ttattcatac
     5341 tgaccgctct agcttcaaga tagttgacct tgacgaagca tggtcttttt tgcaggtggc
     5401 acaaggtaaa acgctatcta tgaaactggt tagagcatcg caagattcac aggacaaacg
     5461 aattgaacgt ttgaagaatt atctgactga aaacctacaa cagttgaata ctgaaatggt
     5521 tcgctcatat attcctacta gttctattgt caacagcttt ctcagtttga gcttactttg
     5581 cagaaaaatg acaactggaa aataataatg gaatagtttc tatgtaaata tatttttact
     5641 atcttaccta actaattgaa agatgatata atttaatcaa agatttggta aggagggatg
     5701 aatatgcatt taattgattt tatgaatgaa gatattaaaa agaagcttgt taaaaaaacg
     5761 tataaaaaga aagagactat tctttttgct gagcaagaaa atgaatatgt aattcttatg
     5821 attgacggta ttgcagaagc attcatctta aatgtgaaag gaaacatctc aactattcat
     5881 ctttatagaa gtggtagttt ttttggagaa atggaacaat ttaatgatgg gaaaaaaccc
     5941 gttgaaattg tcgctttcac agattgtaat gtatataaac ttcatagaga tgatttttta
     6001 aactggttaa ggggagattt tgaagctaca aaatttctga ttcgagaaat atctagtaaa
     6061 ttagttgtaa atgctgaatt aattgaagaa ttatcatctt taagtgttaa agaacgactt
     6121 ctgaggtgta tatccttaca ctattataga agtgaattaa atctgcttac caaaaaacag
     6181 cttgctagcg aagtaaacgc tccaattaga agcattaatc gtgctataaa tgaatgtgta
     6241 caagaacatc taattgctta tgaaaacaaa agatttagtg taattaatca gcgagaggta
     6301 ttaaagaatc ttcctacaac ataagtataa tttcaaaaaa gtattgaata ataattattc
     6361 aatgcttttt tatttcgggt caagtgacct attttttttt attttttcat tatataatac
     6421 tcctatgcat aattatgttg gattgatagc taataaaaga aagtgagagg tgttatacta
     6481 aaaaagaagc atacttccca actccagact tacagtggat tgaagataga ttttggattt
     6541 gggttcatta cgcttgcact aagattggac gtagagaact atttgaaaca attgattttt
     6601 tatctttttt aagacaatca gtacttgccc cattaataca gcttctctta ggtaaattac
     6661 ctcgtggagt tagaaaaatt gaattggacg caccccaata tattgaagca ttagaaaaga
     6721 cggttgctaa ccatgaatat aaaagctgtg ttaatgcatt gcaggctact atcaacctgt
     6781 atcttgagtt aagagaggaa ttatcacaaa gtcctattgt ccgaaaggtt gaagctgaaa
     6841 gggttgcatt acagtatttt caagatttaa tcaaataaac ccaaagtaaa aaaatattga
     6901 atagtaaata ttcagtgttt tttcattttt ggtcaaatga cctagattag ttgttgtaac
     6961 cattctataa ttattttgaa agtaacagta tggaggataa ctatgagtga tattttaagt
     7021 agaattacaa ataaaattgg ggataagaat atagttgata agttatcagc tctatcaaaa
     7081 tcagatttga actcacttct attagaagtg tttgatagac aagcaaatac tttaactgca
     7141 actgatattc taaagtcata ccaactaaat agatttacta tccctagcgg tatagatcca
     7201 gaagaaattc atgctttgga gtcaaaatta ttgagaaaag cattcaatat ggatatcaag
     7261 acgattatgc tttctccctc tgctccatta ggtagctgta gcgtttttgg ctccgtagat
     7321 caatataatg tagtaagtgc tctacgaggg acagaaacac ttgcagaccc ctcaaatatg
     7381 ttggcaataa ttattgctga taaattaaaa agaaaagaag aaaataatac catacctatc
     7441 cacatggcta ctactgctag ggtaataaga gcacaaaatt ttgtaggtaa aggtttatat
     7501 tcacattttg gcttatattg tatggtttca tctggtatag atacagggtc ttacacttgt
     7561 gaaaaaatgt tgttagaaaa gcacttaaac tattataaag atatattaag tgaaatagaa
     7621 aatgttcgtt tctctattat tctacgcaaa agaaacggat acaaagataa tgacggattt
     7681 ttcgacagaa tggttgaaac tatcaaactt atttttccta atatagagtt atcaattcat
     7741 aatgatgatg ttgataataa ttattataaa ggtatcaatt tcaaattatc ggtcaaacaa
     7801 ggaaatgaaa ccgttgaaat agttgacggt ggatttgttg actggactta tcaaatgctt
     7861 ggaaataaaa aagaacgctg tttgataagt ggtattggct tagaaagatt tcttgtagct
     7921 gtttcataaa aggagaagaa aaatggaact tatagagtgg aattatgctt atattaccga
     7981 tttagttgag tttgcgaatg ataaagaaat tgcaaagaat cttcgtgaca acttccccca
     8041 cccttatagg gagcaaaacg ctagagattt tgttagtttt tgtttggcaa ctccagatgt
     8101 taaacaactt agctatgcta ttttttatca agaaagggct attggaagta tcagcctaac
     8161 atttgaagaa gatacctcta ttaataaaaa tgctgaatta ggttactggc ttgctaggaa
     8221 ctattggggc aaagggatag caacaaatgc agtaaaagaa atatgcaaaa ttgcctttga
     8281 aaaatacaac cttaacaaaa ttcatgctaa tgtatattcc tacaatagag ggtcaagtaa
     8341 agtattggaa aaatgtggtt ttaagattga agaatactgt cacaattcat aataaagaat
     8401 agaaacatga ttgattgcca tgtatatagt ttaattcgac cgcaatagca tttattagaa
     8461 ttaacctact ttttaataac agaataagtt tttcccctct attaatttct tatgcatctt
     8521 tgtattttta tgaaaagcta gccaatgcat atttccatac atcaatatgg tggtggctta
     8581 caaccaatat aaattaaata cgttttaaag aatacacatt tttgccctat aggcagagtg
     8641 tgtatttttt tctctttttt ttgaaattta tcgaagattt aacgccctcc caccgtgata
     8701 ggagtaagca aggttattta actccttgcg atggaaagag ggtgaaatct tatggttgat
     8761 gaaaagacaa ttgaacatca attcgacagt ttctgtaaaa cagttttgcg taactatgct
     8821 agagatattt atgacgaaaa taaacgcaga aatgattatt tggtatctct cgaatctttg
     8881 tcacttgcag aattaagtaa attgagtatt ttagatgatt atgattctaa ctacatctgt
     8941 atggtttctt acgactacaa catacgaatt gaagatgtat taatggcaca agcaatcgga
     9001 aaacttacaa aaagaaaaca agatataatt ctactttcat tctttctcaa catgacaaat
     9061 gctgacatag caacattgat ggatttagca gaaaatacca tacattatca caaaacaaat
     9121 gcactaaaag agttgaaaga actcatggag gaacattgaa atgaaaccta ctaaagaagt
     9181 attacctttt acaattattt tacttgctgt acatggcgat gtaaatgcaa taaacggaat
     9241 acttaaacat tttgaacact atatcattcg tctttcacag aaaactttat ttgatgaata
     9301 cggaaatccc tatattcatg tagaagaaga aacaaagcga atgctaggaa caaaactaat
     9361 cactgctatt ttgagattta atttgtctta atctcgccct ctctccatgt atcaagctat
     9421 ttggtacatg gagaatcact aattatttgt ctataaaagc ccataagcta ttgtgatttt
     9481 ttatagacaa atcaatgccg actgttcttt gacaactgaa taaagtagat agatacgttt
     9541 gtgatacaga tagccaacaa atactatacg ccatgacctt aatcaagaga gctactatat
     9601 agtgatttgt gaataacctt tggaacggtt gttttgccgt aacctgtacc acagtataat
     9661 gatacacctg tatgacacgg ttctgcccat aagaatggga ttggttaaaa taccaatgga
     9721 gcttaccaaa gccatctatc tacttctgaa ctacgaacat agaaaggagt gatacattct
     9781 atgaacaata ctgatgtacc tatttgggaa aaatatacct tgacgattga agaagcgtca
     9841 aaatactttc gtattggaga gaaaaaatta cgtaaacttg ctgatgaaaa ccttgattct
     9901 ggctgggtca ttatgaatag caatcgcatt caaataaaga gaaaacaatt tgagaaaatc
     9961 gttgatacac ttgactttat ctaatgaaat tgagccttat atgttataat attcatatga
    10021 cgtatcaagc tcaattcggt tattgagagg agcttcttat catgtcaact gaaaaaagac
    10081 gagataataa agatagaatt ttaagaactg gagagagcca gagaaaagat ggaagatacg
    10141 catataagta tgtatattca tttggtaaac cacaatttgt ttactcttgg aaacttgtac
    10201 ctactgataa acccccaaag ggaaaacgtg atgatatatc attaagggat aaggaaaaag
    10261 caatacaaaa tgaccttgat gatagcattg acactattgg taagaaaatg acagtttgtc
    10321 agttatacgc taaaaagaat ggactaagaa agaatatccg acttaacact aaaaaaggac
    10381 gccattatct tatgaagatt cttaacaagg agtgattctg tgaagagatg tattgtagta
    10441 gtggaatagt caaaaacata atatatatta aaggagagat ttaaatatga ctattccaag
    10501 ttcaaataag gtatatccaa gaagtaatga ttatcaaact gtatatctta aaaatgtaat
    10561 aacaagggaa aatatagaag ttggagatta cacaatatat aatgactttt ataatgaccc
    10621 aagagatttt gagaaaaaca atgtattgta tcattaccct ataaacaatg ataaattaat
    10681 aattggtaag ttttgctcaa tagcatgtaa tgcaaaattt ctaatgacta gtggaaatca
    10741 ctcaatgaaa tcaaaatcca catatacttt tcccatcttt tatgaagaat ggaaactcga
    10801 tatcaaccat ataacggacg cttgggacaa taagggtaac attataattg gaaatgatgt
    10861 gtggataggt tatgattcta taattatgtc aggtgtaaat attggtgatg gtgcaattat
    10921 tggaacaaga gcagtagtta ctaatgacgt tccaccgtat tctattgttg gaggagtacc
    10981 tgcaaaaatt ataaaaaaga gatttaataa tgatacaatt tcaaaattat tagaaattaa
    11041 atggtgggat tggccatata aaaaaattca atcaaacatt aagcatattc aatcaggaaa
    11101 tattgataaa ttagtttaat attttataat ttatttatat taaactttac tttaataatc
    11161 attttataag agctttttca aaatttgtat attat
//
